PRIMER_PRODUCT_SIZE_RANGE=400-1000
PRIMER_MIN_TM=55.0
PRIMER_MAX_TM=65.0
PRIMER_NUM_RETURN=50
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=30
PRIMER_MIN_GC=40.0
PRIMER_MAX_GC=60
PRIMER_MAX_SELF_ANY=6
PRIMER_MAX_SELF_END=1
SEQUENCE_ID=MAGIC.287_1_13439943_13439958_zu-0_oy-0_779744_19199_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17938504_17939062_tsu-0_ct-1_10337_7035_TRUE
SEQUENCE_TARGET=950,658
SEQUENCE_TEMPLATE=TNCTTCCTTTCTCCTCTACTTTAANCACATGTGGGAACCAAATTCTTCGTAACCACTCAATNTGGCTCACAATTTTTCAATATCCAATAAAAAACAGTTTCATATATTTTCTCGTGAGATTATTCAGTTNTTCCTAATATCTATCTATCTCTATATTACATATATACAATATTATATAAATACGCACTCTTCTTTCACCGACCTCCTTACACCAAAAAGGTGTCAATTCCACTTGTTCACCATGAATATGATTTAATGGGTTCTTCTTCTTTTGCAGCTTTCTTTCTTTTCCTTAAATTATCATTGTTTTAGGTGGGGTTTTNAGTGCTTNCATGACTCATTCATTCTCTGTATAAAACCTAGTGGAAAGTGTTAAATCAAAAACCCTTTTTTCCCACTTTCTTAATNNAGAAGGGAGANNAAAAAAATGGTTAGAGTNATGTTGGTGAGGTTTGGGTTTTTATTACTTNTGATCTCTTTTGTGTTTTTGTCTAATAANACTTTGGGACAACAACAAGACGATGATGATGATTCTGCTGTGTACATTGTCACACTTAAACAACCTCCTANTGTNCATCNNTTTGAAGAACAAGAGCTTAAACACAAAAANTCAAAGTTTACNCCAAANTTGNNACCAAGGTACTGATNAAATTNNATTNTTTTTGTTGTCNNNNNNNNNNNNNNNNAGCTTTAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNTTATAGGAACAATNCAAGGAAACGTCATGGGAAGNCAAAGATACCATCTGTTGTTCAATCTCANGACTCTTTCTTGAGAAAGACTNTAAAAGGAGAGAAGTATATAAAGCTTTATAGTTACCATTATCTNATCAATGGATTTGCTCTGTTNATTAACTCACAACAGGTAAAAATCTTGAGTTNTTGATAATTGTAGAAATAATCTTGAGTTCTTGATCTTGATTTTTNTGTGTTATGGTTTTNNTTTTTNTAAGGCTGAGAAGCTTTCAATGAGAANNGAAGTAGCAAACATAGTGTTGGATNACTCTGTTAGGANAGCAACAACTTATACTCCACAGTTTATGGGNTTACCACAAGGAGCATGGGTCAAAGAAGGTGGATTTGAGATTGCTGGAGAAGGAGTTATAANCGGTTTTATCGATACTGGGATCGATCCNAATCATCCTAGTTTCAATGACAATGACTCTAAGCGTTCATATCCAATCCCTAAGCATTTCTCAGGTGTTTGTGAAGTTACACCGGANTTTCCATCAGGATCTTGCNATAANAAGCTTATTGGAGCNCGGCATTTCGNGCAATCCGNTGTAACCAGAGGAATCTTTAACNCATCTGAAGANTACGCTTCTCCTNTCGATGGAGATGGACATGGAACGTAAGTGTAACTNTTGAATGTATCATNTAAAAACAGAGTTTGAAGCTTATAGATTGTTGCTTTGGATNTATATGCAGACACACAGCTTCGNTTGCAGCGGGTAACCACGGAGTTCCAGTGNTAGTTTCTAACCNTAACTTTGGATACGCTAGTGGAATCGCTCCTCGTGCATTGTAAGTAAAANANNAANANTTNGATCAANGTCGAAATCTNNACNAAACNAACTTNAACNTTTATGTTNNNNNNNTTTNTTTTNAGTATNTCTGTTTACAAGGCATTNTATAAGNGTTTTGGAGGTTTTGCTGCAGATGTTGTNGCAGCTATAGATCAGGTTTACAAATCAAGAAACTAAATGTGAATAAANATTTTAGCTTTTCTTTCAAATNTGAAGACTTTAATATAATNGTTGTGANNTTTTTTCAGGCAGCTCAAGATGGAGTAGATATANTAAGNCTNTCAATTACACCGAATCGNAAACCTCCCNGTGTTGCCACTTTCTTTAATCCTATCGANATGGCNTTACTTTCNNCTGTAAAAGCCGGAATNTTCGTAGTCCAAGCNGCGGGAAATACCNGTCCAGCNCCTAAAACCATGTCTTCTTTTAGTCCTTGGATNTTCACNGTTGGNGCTTCNTCNCATGATAGAGTTTACTCNAATTCTTTAACCTTAGGAAACAATGTAACTATTCCAGGNATNGGATTCGCAAGTAAGTCNNTACCATCTCAAACTATAACTTTCNANGATTCAAGTCNAAGATTANGGATTNNNNNNNNNTGTANTTTGTTTCTTTATAGNTCCTANNGATNNTGGAAANATGTACAAGATGATNTCNGCTTTTCATGCCTTGAACAATAGTACTTCTGTAGATAAAGATATGTATGTAGGTGAATGTCAAGATTATGAAAANTTCNATCAAGATCGTGTCTCNGGGAANCTTTTGATATGTAGCTACTCTGCTCGNTTTGTTCTCGGNCTTTCGACTATAAAACANGCTTTAGATGTTGCCAAGAATCTATCNGCGANNGGNGTGNTATTNTATATAGACCCGTATGTTCTTGGTTTTGAGATCAATCCAACACCGATGGATATGNCCGGGATNATAATTCCATCTGTAGAAGATTCCAAGGTGAGAAAACTAAAGCATTTGTTTAAAGGTTTATACAAAGTTTCTAATGATCTAANG
PRIMER_LEFT_NUM_RETURNED=26
PRIMER_RIGHT_NUM_RETURNED=26
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=26
PRIMER_PAIR_0_PENALTY=0.748927
PRIMER_LEFT_0_PENALTY=0.486072
PRIMER_RIGHT_0_PENALTY=0.262854
PRIMER_LEFT_0_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_0_SEQUENCE=ACATCTGCAGCAAAACCTCC
PRIMER_LEFT_0=737,20
PRIMER_RIGHT_0=1686,20
PRIMER_LEFT_0_TM=60.486
PRIMER_RIGHT_0_TM=60.263
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=950
PRIMER_PAIR_1_PENALTY=1.261790
PRIMER_LEFT_1_PENALTY=0.486072
PRIMER_RIGHT_1_PENALTY=0.775718
PRIMER_LEFT_1_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_1_SEQUENCE=ATCTGCAGCAAAACCTCCAA
PRIMER_LEFT_1=737,20
PRIMER_RIGHT_1=1684,20
PRIMER_LEFT_1_TM=60.486
PRIMER_RIGHT_1_TM=60.776
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=948
PRIMER_PAIR_2_PENALTY=1.560084
PRIMER_LEFT_2_PENALTY=0.486072
PRIMER_RIGHT_2_PENALTY=1.074011
PRIMER_LEFT_2_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_2_SEQUENCE=CAACATCTGCAGCAAAACCT
PRIMER_LEFT_2=737,20
PRIMER_RIGHT_2=1688,20
PRIMER_LEFT_2_TM=60.486
PRIMER_RIGHT_2_TM=58.926
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=952
PRIMER_PAIR_3_PENALTY=1.662348
PRIMER_LEFT_3_PENALTY=0.486072
PRIMER_RIGHT_3_PENALTY=1.176275
PRIMER_LEFT_3_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_3_SEQUENCE=TGCAGCAAAACCTCCAAAAC
PRIMER_LEFT_3=737,20
PRIMER_RIGHT_3=1681,20
PRIMER_LEFT_3_TM=60.486
PRIMER_RIGHT_3_TM=61.176
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=945
PRIMER_PAIR_4_PENALTY=1.691478
PRIMER_LEFT_4_PENALTY=0.486072
PRIMER_RIGHT_4_PENALTY=1.205406
PRIMER_LEFT_4_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_4_SEQUENCE=ACAACATCTGCAGCAAAACCT
PRIMER_LEFT_4=737,20
PRIMER_RIGHT_4=1689,21
PRIMER_LEFT_4_TM=60.486
PRIMER_RIGHT_4_TM=59.795
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=953
PRIMER_PAIR_5_PENALTY=1.708627
PRIMER_LEFT_5_PENALTY=0.486072
PRIMER_RIGHT_5_PENALTY=1.222554
PRIMER_LEFT_5_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_5_SEQUENCE=ACAACATCTGCAGCAAAACC
PRIMER_LEFT_5=737,20
PRIMER_RIGHT_5=1689,20
PRIMER_LEFT_5_TM=60.486
PRIMER_RIGHT_5_TM=58.777
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=953
PRIMER_PAIR_6_PENALTY=1.785027
PRIMER_LEFT_6_PENALTY=0.486072
PRIMER_RIGHT_6_PENALTY=1.298955
PRIMER_LEFT_6_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_6_SEQUENCE=CTGCAGCAAAACCTCCAAAA
PRIMER_LEFT_6=737,20
PRIMER_RIGHT_6=1682,20
PRIMER_LEFT_6_TM=60.486
PRIMER_RIGHT_6_TM=61.299
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=946
PRIMER_PAIR_7_PENALTY=2.308000
PRIMER_LEFT_7_PENALTY=0.486072
PRIMER_RIGHT_7_PENALTY=1.821928
PRIMER_LEFT_7_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_7_SEQUENCE=CAACATCTGCAGCAAAACCTC
PRIMER_LEFT_7=737,20
PRIMER_RIGHT_7=1688,21
PRIMER_LEFT_7_TM=60.486
PRIMER_RIGHT_7_TM=60.822
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=952
PRIMER_PAIR_8_PENALTY=2.337253
PRIMER_LEFT_8_PENALTY=0.486072
PRIMER_RIGHT_8_PENALTY=1.851181
PRIMER_LEFT_8_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_8_SEQUENCE=TCTGCAGCAAAACCTCCAAA
PRIMER_LEFT_8=737,20
PRIMER_RIGHT_8=1683,20
PRIMER_LEFT_8_TM=60.486
PRIMER_RIGHT_8_TM=61.851
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.8000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=947
PRIMER_PAIR_9_PENALTY=2.535810
PRIMER_LEFT_9_PENALTY=0.486072
PRIMER_RIGHT_9_PENALTY=2.049738
PRIMER_LEFT_9_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_9_SEQUENCE=AACATCTGCAGCAAAACCTC
PRIMER_LEFT_9=737,20
PRIMER_RIGHT_9=1687,20
PRIMER_LEFT_9_TM=60.486
PRIMER_RIGHT_9_TM=57.950
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=951
PRIMER_PAIR_10_PENALTY=2.798199
PRIMER_LEFT_10_PENALTY=0.486072
PRIMER_RIGHT_10_PENALTY=2.312126
PRIMER_LEFT_10_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_10_SEQUENCE=CATCTGCAGCAAAACCTCCA
PRIMER_LEFT_10=737,20
PRIMER_RIGHT_10=1685,20
PRIMER_LEFT_10_TM=60.486
PRIMER_RIGHT_10_TM=62.312
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=949
PRIMER_PAIR_11_PENALTY=3.039986
PRIMER_LEFT_11_PENALTY=0.486072
PRIMER_RIGHT_11_PENALTY=2.553913
PRIMER_LEFT_11_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_11_SEQUENCE=AACATCTGCAGCAAAACCTCC
PRIMER_LEFT_11=737,20
PRIMER_RIGHT_11=1687,21
PRIMER_LEFT_11_TM=60.486
PRIMER_RIGHT_11_TM=61.554
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=951
PRIMER_PAIR_12_PENALTY=3.506863
PRIMER_LEFT_12_PENALTY=0.486072
PRIMER_RIGHT_12_PENALTY=3.020791
PRIMER_LEFT_12_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_12_SEQUENCE=ATCTGCAGCAAAACCTCCAAA
PRIMER_LEFT_12=737,20
PRIMER_RIGHT_12=1684,21
PRIMER_LEFT_12_TM=60.486
PRIMER_RIGHT_12_TM=62.021
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.8000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=948
PRIMER_PAIR_13_PENALTY=3.525584
PRIMER_LEFT_13_PENALTY=0.486072
PRIMER_RIGHT_13_PENALTY=3.039512
PRIMER_LEFT_13_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_13_SEQUENCE=CTGCAGCAAAACCTCCAAAAC
PRIMER_LEFT_13=737,20
PRIMER_RIGHT_13=1682,21
PRIMER_LEFT_13_TM=60.486
PRIMER_RIGHT_13_TM=62.040
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=946
PRIMER_PAIR_14_PENALTY=4.078241
PRIMER_LEFT_14_PENALTY=0.486072
PRIMER_RIGHT_14_PENALTY=3.592168
PRIMER_LEFT_14_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_14_SEQUENCE=ACAACATCTGCAGCAAAACCTC
PRIMER_LEFT_14=737,20
PRIMER_RIGHT_14=1689,22
PRIMER_LEFT_14_TM=60.486
PRIMER_RIGHT_14_TM=61.592
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=953
PRIMER_PAIR_15_PENALTY=4.525432
PRIMER_LEFT_15_PENALTY=0.486072
PRIMER_RIGHT_15_PENALTY=4.039359
PRIMER_LEFT_15_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_15_SEQUENCE=TCTGCAGCAAAACCTCCAAAA
PRIMER_LEFT_15=737,20
PRIMER_RIGHT_15=1683,21
PRIMER_LEFT_15_TM=60.486
PRIMER_RIGHT_15_TM=63.039
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=947
PRIMER_PAIR_16_PENALTY=4.535427
PRIMER_LEFT_16_PENALTY=0.486072
PRIMER_RIGHT_16_PENALTY=4.049355
PRIMER_LEFT_16_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_16_SEQUENCE=ACATCTGCAGCAAAACCTCCA
PRIMER_LEFT_16=737,20
PRIMER_RIGHT_16=1686,21
PRIMER_LEFT_16_TM=60.486
PRIMER_RIGHT_16_TM=63.049
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=950
PRIMER_PAIR_17_PENALTY=4.991426
PRIMER_LEFT_17_PENALTY=0.486072
PRIMER_RIGHT_17_PENALTY=4.505354
PRIMER_LEFT_17_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_17_SEQUENCE=CATCTGCAGCAAAACCTCCAA
PRIMER_LEFT_17=737,20
PRIMER_RIGHT_17=1685,21
PRIMER_LEFT_17_TM=60.486
PRIMER_RIGHT_17_TM=63.505
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=949
PRIMER_PAIR_18_PENALTY=5.627366
PRIMER_LEFT_18_PENALTY=0.486072
PRIMER_RIGHT_18_PENALTY=5.141293
PRIMER_LEFT_18_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_18_SEQUENCE=ATCTGCAGCAAAACCTCCAAAA
PRIMER_LEFT_18=737,20
PRIMER_RIGHT_18=1684,22
PRIMER_LEFT_18_TM=60.486
PRIMER_RIGHT_18_TM=63.141
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=948
PRIMER_PAIR_19_PENALTY=6.181225
PRIMER_LEFT_19_PENALTY=0.486072
PRIMER_RIGHT_19_PENALTY=5.695153
PRIMER_LEFT_19_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_19_SEQUENCE=TCTGCAGCAAAACCTCCAAAAC
PRIMER_LEFT_19=737,20
PRIMER_RIGHT_19=1683,22
PRIMER_LEFT_19_TM=60.486
PRIMER_RIGHT_19_TM=63.695
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=947
PRIMER_PAIR_20_PENALTY=6.645668
PRIMER_LEFT_20_PENALTY=0.486072
PRIMER_RIGHT_20_PENALTY=6.159595
PRIMER_LEFT_20_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_20_SEQUENCE=ACATCTGCAGCAAAACCTCCAA
PRIMER_LEFT_20=737,20
PRIMER_RIGHT_20=1686,22
PRIMER_LEFT_20_TM=60.486
PRIMER_RIGHT_20_TM=64.160
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=950
PRIMER_PAIR_21_PENALTY=6.645668
PRIMER_LEFT_21_PENALTY=0.486072
PRIMER_RIGHT_21_PENALTY=6.159595
PRIMER_LEFT_21_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_21_SEQUENCE=AACATCTGCAGCAAAACCTCCA
PRIMER_LEFT_21=737,20
PRIMER_RIGHT_21=1687,22
PRIMER_LEFT_21_TM=60.486
PRIMER_RIGHT_21_TM=64.160
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=951
PRIMER_PAIR_22_PENALTY=6.645668
PRIMER_LEFT_22_PENALTY=0.486072
PRIMER_RIGHT_22_PENALTY=6.159595
PRIMER_LEFT_22_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_22_SEQUENCE=CAACATCTGCAGCAAAACCTCC
PRIMER_LEFT_22=737,20
PRIMER_RIGHT_22=1688,22
PRIMER_LEFT_22_TM=60.486
PRIMER_RIGHT_22_TM=64.160
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=952
PRIMER_PAIR_23_PENALTY=7.062443
PRIMER_LEFT_23_PENALTY=0.486072
PRIMER_RIGHT_23_PENALTY=6.576370
PRIMER_LEFT_23_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_23_SEQUENCE=CATCTGCAGCAAAACCTCCAAA
PRIMER_LEFT_23=737,20
PRIMER_RIGHT_23=1685,22
PRIMER_LEFT_23_TM=60.486
PRIMER_RIGHT_23_TM=64.576
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=949
PRIMER_PAIR_24_PENALTY=7.248079
PRIMER_LEFT_24_PENALTY=0.486072
PRIMER_RIGHT_24_PENALTY=6.762007
PRIMER_LEFT_24_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_24_SEQUENCE=ATCTGCAGCAAAACCTCCAAAAC
PRIMER_LEFT_24=737,20
PRIMER_RIGHT_24=1684,23
PRIMER_LEFT_24_TM=60.486
PRIMER_RIGHT_24_TM=63.762
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=43.478
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=948
PRIMER_PAIR_25_PENALTY=8.252677
PRIMER_LEFT_25_PENALTY=0.486072
PRIMER_RIGHT_25_PENALTY=7.766604
PRIMER_LEFT_25_SEQUENCE=CAAGGAAACGTCATGGGAAG
PRIMER_RIGHT_25_SEQUENCE=ACAACATCTGCAGCAAAACCTCC
PRIMER_LEFT_25=737,20
PRIMER_RIGHT_25=1689,23
PRIMER_LEFT_25_TM=60.486
PRIMER_RIGHT_25_TM=64.767
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.826
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=953
=
SEQUENCE_ID=MAGIC.426_3_15023568_15024025_zu-0_no-0_310766_105471_TRUE
SEQUENCE_TARGET=950,557
SEQUENCE_TEMPLATE=ACGAGAATNGNNAAGACGTAAAAAANNNTGATAAATTTTAANTNANNAGAGTTCTCTTGCATTANGAACAATTAACGTNATAATCTAGGTAACAANGANNAATNNTTTCCANGTAAAAATGAANGAGAAAAAGATAAANGTNNANAGATTTGNGACAANAAANANAGATATAAAGAGNAANCNATTTTNTTNATGNAGAANAGGTCATATTNTGACANTANAAGANAANCTTAAANAATTNGTTNNGAGNAAAGGTGAGAAAANAGTTATGACNTGAACTCNTTAAANCAACATTNGGTTCATATATTTAGNANTTTCTGGTATNANNATNTTTANGTTNAATNNTGGATATACATTCAAACNNTTTNNNNTNTGTGTATTTTATNTAAGCTTNTTCAATCTGTTTGNNAAANAAAAATCTTNTAANGTTTTAAATATGANANNCATCAAACAAACATNCAAACATAATTNGANTAATNTNAAATTTNGTAGGAGACGTGGAACTAAGTNTNGNTANAAAGANATCAAATTTTGAGTTCAAAATGTTAAATAAACAACACTGGTCNANTTTGTTTAATTTATTTAGAAGCTTATAGAACTATNTTGGGTTAAGGTTAGTATGTTTAAATTNATTCTTGTTTTCAACACATAATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTNGACAACAACTTGTTNCATTCCANNAAGTAAAAANCCAAAGATCAAGACAAAGACGAAGGGATNCNGAGATCCCAAAGTTTAGTTTACAACAAAGAAGGGCATTCCCCAATGCCNACANAAANAGATAANNGNATAAANAAGGGTAATCGNTNATACAATCTTNAGAGCTTTGAGCCACAAGCGGTGAGGCATGATGGTACATNTGAGGTCAAGAATTAGCGATAAATCGTCCAAGCGTGAAACANTGGAGGACCAAATAGCCATGGTCAATAGACAGTTTGACTTNNNNNNNGGCGTCAAAGTCTGGGATCTCNCCGGCACGAGGAGGACTACACCANAAACGGCTTTGACAAGGTTTAGAGCTACGGAGATGATGNNCTGCTGAATNAGGTTNAAAGCTGTCATTGTCNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13151073_13151732_tsu-0_oy-0_1453_2081_TRUE
SEQUENCE_TARGET=950,759
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAGAGTTTCGGCTANTGAAGCGTTGNTAGCNTCTTCTCCAGACNTTGCTNCTCACTTCGGAAATGTANTANATAGAANAAATNTNANNACTCAAGAATCCTNCGTCCCCACAGANGNCGCCAAAATNTGGATGTCGGATTCACACACTCAANTATTTATTTNTTTANTGAGAAGGATTATTCTTGAGGACAAATGTACTAGAAAATANANNAATGATTATAGAGAAAACTATGAGTTTTGTAAGAAAATNNNNNANAATCCANTATATAAAANCANATGTTCAAAAACAAATATGCCTAAGAAAAGGGAAATTTGGTCGTTTGTACCACATTCACNAAAAAAANCTANCCGTTTGTANCACATTCATGGAGACATNCAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_8030731_8030773_tsu-0_po-0_5446_305111_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGAACNAACCCAAGAANTNTAGTAGAACTGTNGGTTTNACAATTTACACNNNNNNNNNNTGGCTTATTGGCTTAGATAGGAACAGATGTTCACTTTTACGATATCTAAATGCTTAGTAGATGACACTTGGGCGGTCGATGNGGACTATTTCGAGTCANCAAACGAAGTGACAAATGATTGAAATCGATAAGAGACAAAAAGTTTAATTAAAATTGAATATTTGTCCTTTACTTTTCTCAGACAACAAAACATATACAGAGATGCGTCTCTCTCTATCTTCAGTGTTTTTAGGGTTTTTAAGACTGAAGCTGAATTCACAAGATGAAATGGTCAGAGAGACTATTGAAGTTTGGAGAATGGGTCCATAACTTGAAAAACAAGCCACCTCTGTTCCATCACTGATTACTCCTCTGTCTTTTTCTTGTTTAGAGTAAAAAGCAACCTCNAAGNTCAGAAGTAAAATTATTGAGATTTTACAGCACAGTTTTGGTAAATATTAAAGCACACGGCATCAGAGATTCTGCAACACAAACAAGAAATAACATCACTAAAAACCAGACACACATTGGCACTAATTTCTACGCTCTCTCTCTCTCTAAACCGAAGATAAGAGATTCTCTGCAGACAAGACAATATAGAATATTAATTGTGATCTCTCTCTCACCCTCTCNCTTTCTCTTTCTCTCTCTCCCTATTAGGTTTCATTGAATCTAAAACGGAAAATCCTCGGGATTACTNNCCAGTAAAGAAAGGGTTTACATATAGTTGNATTGGTCCGAAACTGAGGCGATGATTATTATCAACCTTGGTGTGGTGGTGGTGGAGGCATAGACATGTGATGGTGTTGGTATGGTGGTTGTTGTTGTTGTGGTGGTCCACCTTGCTGCTGCATGTGTTGCGGTGGCCATCCTCCATGTTGGTGCATTCCCATGGCTTGAGGTGGTGGCGGCGGCCTCGGACCACCGAAACCTTGTTGGTGATTTAGAAACTGAGGAGGTGGTGGCGGAGGCATACCCTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_6697795_6697978_po-0_edi-0_31510_13846_TRUE
SEQUENCE_TARGET=950,283
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTCNATTTTTTCTTTNNNACTCNAAACGNTTTTTCGATAATAAAATNTNGTAACGCATAACATNGATNAAANNNNNCTTTTTNNANTCTTTAAAGGTGGANNAGAGTNNNNNAACGANGACNNATGATANCTNNNNNNNNNNNNNNNNNNNNATCTTCNTNNCACATAAANTANNCTNGAGAANANATCANANTGATATAANNNAAATTAGAGNCTTAATGATTTTAAGTNATACAAGNNTATANGTAAATTTCATTTTANTTAATATTATAAACCATACAAACCTAGTAAANGTTNTAANAAAANTAACATNTTAATACATAAGAAGAGGACACAAGNGTAAACTNTAAAGAGGGANCGGNTAGCATTTTTTCCTTAGATTNTAAAAACTCAATAATCCAATNAAATCAAATGTGATAAAATAGAACTAAAATTCATCATATAGCTTAAGCCCCCCATATATAAGATCCTCACAATGCCTAACAAAAGGAAGAATCTTCCCCGAGTTAGTTCCCNAGTCAGCTTCACATCCATGAGACGGAGGAGTNAGGTAACATGGCTCCATCGACTTAGATCGAACACATGGCGGATTAGGAGTGGTGAAATTTGTTGGCTGATAAAGTAACCATGGCTTTAATCCTCCAAGACTATATGAAACATATCCAAATGTAGACCTTGCGCTCGTGACAATGTTATCGGTTAAGCTCAAAAGATACATCTCAGCAAGCGCCTTTTGGTCGTGACTTTTCTTGTCTGTTTGCTGAACTCTCTCTCCACTTGGNTGATNAACTTCAACAATTTCTCCTGTTGAACTTGGTCGCTTNGAAAACATNTTNGTTAAGTTGCCAGAGAACTCTGGATAGAGAGATGCGACAAGAACAGATTTGAGTTTCGNGNNTTTTGATATATTGACTTTTGATTCCTCTGGTGTAGCAAATTCAGGTAACAGTTTCTCTCTTTGTGTACATGNTANGACCTGGTCAATGACATGTTTGAAACGTCCATCAGGTTTNCCGAAAACCCTTATTTGAATCCCGAGTCTCTCGTCGGCTTTCGACAAATGAGCGTNGTAGTAGTCAGTGACCATATCCCAAACTTCATTTGTAGGGTGAAAAAGATANCGAGCCAAGTGGTGAAAGACGGTTTCTTTCTGCGGGAACAGNTTAACTAGTTCGGTTTGGAAAGTTGGATTAAACCATAACGATGGAACAAAGTAAACATTGGCTTGAANAATCAACCATGGGACTTTGTCAATCAAACTTTGATCCTTTTGGCANACAAACAGCTTATCACTATCCCTTGAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_8292121_8292129_can-0_ws-0_7759_53147_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACATCTNGGTGTTGNNCAAGATGTGGACGGGAATACACNNNNNNNNCTTGCCGTCATGAACTGGCACTTNNANTCCANTACTTGNCTTGCNAGNAGTAGCAAGATACTGNANNNNNNNAACAAAANNGGCTTAAGAGCTAGGGATATTGCCGAGANAGAGGTGAAACCCCACTACATNTTTNAGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANANCACATANATGNTTTANNNNNNNNNNCCCANNTTTTGNNTTANAGAGGTGGACANTGGCNCTCTTNTTANACNNNANTCACTNAAGNGGTTTCGAATCTGTNNANTCNTTAACAANACCGNCAGNNCCACTAGACCCTAANAATAACAGAGATTACGTNAACACTCTTNTNNTGGTAGCNGCTCTTGTAGCNACNATGACGTTTGCTGCAGGTTTTACNNTACCNGGTGGNTNTAANAGCTCTGCTCCANACTTGGGCAGGGCNNCTTTGGCCACTAANCCAACTCTCTNNATTTTTCTGNTACTTGACATCTTGGCNATGCAAAGTTCCNTTGCAACNATAGGTANTCTTATTTGGGCGCAGTTNGGTGATCCAGNNCTCATNCGNTCATCCTNNCATGTGGCNTTGCCCTTACTGCTTTTTGCTTTACTCTGCATGCCCNTGGCATTCCTTTTTGGNGTGNTNACNGCANTTGGNCANGTGAAATGGCTTNTAGTCATCATTTGCNTTATATCTNNTNTATTCNNNTNTNGGGCGATCTTTGTCCTTGGNCCNCACGTCATGCTACANCGNNCATACNTTTCTCCCANNTANNNNNNNNNCTTTCTCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTNTGGTTGTTNNTNAGANTTTGTNGNNTATNNNNNNNNNNNCNNCGTATGAANAAATNATNNNNNNNNNNNNNNNNNNNNNAGATTTTTGNNNNNNTNNANAANAAAANNAAAAAGCTAGATATNATGNTCTCTCTGAGNAGCGGNATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANACACATGATTCGATTGCTAACCAACACAAGAAACAAAACAAACACATCTTTGCCTATAAGATNANNTNTATAAANCATACANCNAGTGANNTGANGGAGGACAATGAGAAGACNATTTCATTATGNTGAANCGTCCTTGACGTCTTAGCAGTTANNNCTGTAAANTGGGTTCANATCANANTAAANGAATCACTNTACACAACAATTACATGCTAAAAACTNAGAAGCTAAGTTACCAAATTCTNNATAGAAATANCAATCACTTACAATAANCAATGCTNNATAACAACTTTTGAGTCCATAACTGATAAATTGATAGAAACTGTTTNAATTTGCANANCCAAAACCGTTTCTTAGGATGGAGNAATNGGNTTGTANTTTTCTATTTCCGATCAAGGAAANATATNTCAGGCAACAACNGNGNANGATNGNNCATTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9106319_9106986_tsu-0_po-0_48325_48592_TRUE
SEQUENCE_TARGET=950,767
SEQUENCE_TEMPLATE=ATCCGGTTGTGATGATTATTAATCGTGNAAAAANATCAGGGATTATAACNTTTGTTAAATTGTGAACNNACAATATTGAACTGAAAATGCCAAATGAATTGGTTATTANTACTTTGATGAAAATGAATTGATCGTTTANNTTTTTTTNTNTTTATAACNNAAATGCGATNTGCTCTTTTTAATTCTTTATTTCTATTTTTGCTTCAGAANTTTTTTTCATTTTATTTTTGGTCAAAAAGNTTATTNATTTCCTTTTCAATTTGTACGAATTTAAACGGTGACGNTTTNGTTTAAATTATCAAACAAATCAAAACAAAATCAATAAAANNNNNNNTCTCATCATGAGGCATCTTTGACTGCTCTTTAATTCACTAAAAAAGTCTAAAACCCTAACCATTGCCCTTTTTCAAACGAAGAAGAAGAAGACGAAACGCTACCATCATCTACACACACACNCNCAACTACTTTTTTCCATCGCCGGNCTGTACAAATGTCNACTACCCGTAGGATCCGNGGCGAGAATCGGTTTTATAACCCACCTCCGATGAGAAAACTTCAACANGAGCGTGAGAAGAAGAGACTTGAAGCGGANGAGATAGNGAAAGAGAAGAAGAAGGCAAAGGAGATNCTCGATCNAAANATTANAGNCNAGGAGAAGGAGATTAAGCAACCTGAGGAGTGCTCGACCTCCGATTGCTCTGTGCCGAGTCGGGTCTCCTCCACCACCACCACCACCGGAACNACTTCTTCCAATTTGGGCCGGTTTCTGGATTGCACCACTCCAATTGTTTCTANGCAGNACTTGCCTTTGGTAATTACTCCGATTTTGAAGTTTTACTTGAATCGACGACTTGGGTTTGGGCAGTTTTANAATGAGAACATTAGCATCAAGCGTTGAAANTGGNAANTTTTTATCAATTCTTGGGAACGATTATGATCTCAGTCTCTTATTGAAGATAGAATTGGTTTCAGGTTTTAGAATTTATCGCTATTTAGGNTTNTAGATTTGAGAAACAGATTCAGTTAGTAGAACAATTGTGATGTTGTTGTNGTCAGTCATAGTTTCTGTGTTTTTGCTTCAGACAAGTAGTAAGGGGTGGAGAACTCGTGAACCAGAGTACCGTCCCTATTTCTTGCTGAATGATTTGTGGGATTCATTTGAGGAATGGAGCGCCTACGGTGTCGGGGTTCCTCTTCTTTTAAATGGGATNGATTCGGTTGTTCAGTATTATGTNCCTTATCTNTCTGGNATTCAGCTTTATGAAGANCCATCTAGAGCCTGCACAACTAGGAGGTGAGTTCTTNCATTCTGCTTATATTTTTNNTTGTTTCCTATCAANCATTAGCAGNAGTGATCNGGTTCTTTGCTCNTTGATTNGNNNTTCTTCTGATAGAAAAAAGGATAGCAGTTNNNNTTTTTGTCAATTTATATATTTTGAGATGAAGCCACTTCTTTCTTGGGTTGTTCTTNATCTTNGGATGCACGTTCTTTAGGNGATAGGAAAACCAACTTCTAAGCGTTCTNTGCTACAATATCTAGAGATATTCATTTGGATGACTAATACACAACAACTTTTATGCTCTCAAATACTAGTCNGTGGTTCAAGTTTATCATGGTGTTGAATTCCAANTTTGTTTNATCTCGTTGGTTTTCTCTTTAATTCCCANTTCGTTTCTNCTTTCTAGAGTAAAATTCGTTGTTTTGATGTTTGTTCTTAAACAGGAGAGTTGNTGAAGAAAGTGATGGTGATTCGCCTAGAGACATGAGTAGNGATGGGAGCAATGANTGTAGAGAGCTTTCTCANANTTTATACAGAGCCTCTTTGGAAGAGAAACCTTGCATTGGTTCATCGAGTGATGAGAGTGAGGCCAGCAGTAACTCTCCTGGTGAACTTGTCTTTGAGTATCTTGAGGGTGCTATGCCATTTGGCCGTGAACCNTTGACCGACAAGGTANGTTTATTATGTAANNAAAGTGGNTCAACTTCTCACANTGGTGTTCAATTTCATAGCCNGCGAAANTGTTCTGTCCTGTNTCATANTAATGTTTAACCTTAATATTGCAGATCTCAAACCTGTCATCACAATTCCCANCACTCAGAACATACAGAAGCTGTGATCTATCACCTTCGAGTTGGGTCTCTGTTGCTTGGTACTGCATCTAACATGTCCNTTCCACATTTTCATGAGTATATTTTTTCCAATCACTCTCTATAAATATTGATTTCTTCTCGCATTCTTGAAGGTACCCAATATACCGAATACCATTAGGTCAATCGTTACAAAACCTNGATGCTTGCTTTTTAACATTCCACTCGTTGTCAACACCCTGTCGAGGTAGGCCTGCTCTGCATATTTAAAACATAGTCTATANATGGGCTTATTANAAACATCGCAAATCAACTCTTGTATTACTGGTAAAAGGTACANGCAATGAGGAAGGTCAAANCTCAAGCAAATCGGTTGCATCGNCGAAGCTTCCATTGCCCACTTTCGGTCTCGCATCCTACAAGTTTAAACTNTCAGAATGGAGCCCAGAGAGTGACGTAGACGAGAACCAGAGAGTCGGGACGCTGCTACGAACAGCTGAAGAGTGGCTCAGGCGGTTGAAGGTGATTCTACCGGACTTTAGACACTTCATATCGCATAGTGGTTCGGNNTGGAGATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.470998
PRIMER_LEFT_0_PENALTY=0.026532
PRIMER_RIGHT_0_PENALTY=0.444466
PRIMER_LEFT_0_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_0_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_0=761,20
PRIMER_RIGHT_0=1756,20
PRIMER_LEFT_0_TM=59.973
PRIMER_RIGHT_0_TM=59.556
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=996
PRIMER_PAIR_1_PENALTY=0.496517
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.469985
PRIMER_LEFT_1_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_1_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_1=761,20
PRIMER_RIGHT_1=1755,20
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=60.470
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=995
PRIMER_PAIR_2_PENALTY=0.555543
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=0.444466
PRIMER_LEFT_2_SEQUENCE=CTTGAATCGACGACTTGGGT
PRIMER_RIGHT_2_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_2=837,20
PRIMER_RIGHT_2=1756,20
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=59.556
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=920
PRIMER_PAIR_3_PENALTY=0.555543
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=0.444466
PRIMER_LEFT_3_SEQUENCE=ACTTGAATCGACGACTTGGG
PRIMER_RIGHT_3_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_3=836,20
PRIMER_RIGHT_3=1756,20
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.556
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=921
PRIMER_PAIR_4_PENALTY=0.581062
PRIMER_LEFT_4_PENALTY=0.111077
PRIMER_RIGHT_4_PENALTY=0.469985
PRIMER_LEFT_4_SEQUENCE=CTTGAATCGACGACTTGGGT
PRIMER_RIGHT_4_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_4=837,20
PRIMER_RIGHT_4=1755,20
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=60.470
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=919
PRIMER_PAIR_5_PENALTY=0.581062
PRIMER_LEFT_5_PENALTY=0.111077
PRIMER_RIGHT_5_PENALTY=0.469985
PRIMER_LEFT_5_SEQUENCE=ACTTGAATCGACGACTTGGG
PRIMER_RIGHT_5_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_5=836,20
PRIMER_RIGHT_5=1755,20
PRIMER_LEFT_5_TM=60.111
PRIMER_RIGHT_5_TM=60.470
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=10.0000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=920
PRIMER_PAIR_6_PENALTY=0.897888
PRIMER_LEFT_6_PENALTY=0.453422
PRIMER_RIGHT_6_PENALTY=0.444466
PRIMER_LEFT_6_SEQUENCE=TGCACCACTCCAATTGTTTC
PRIMER_RIGHT_6_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_6=772,20
PRIMER_RIGHT_6=1756,20
PRIMER_LEFT_6_TM=59.547
PRIMER_RIGHT_6_TM=59.556
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=985
PRIMER_PAIR_7_PENALTY=0.923407
PRIMER_LEFT_7_PENALTY=0.453422
PRIMER_RIGHT_7_PENALTY=0.469985
PRIMER_LEFT_7_SEQUENCE=TGCACCACTCCAATTGTTTC
PRIMER_RIGHT_7_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_7=772,20
PRIMER_RIGHT_7=1755,20
PRIMER_LEFT_7_TM=59.547
PRIMER_RIGHT_7_TM=60.470
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=984
PRIMER_PAIR_8_PENALTY=0.939639
PRIMER_LEFT_8_PENALTY=0.495173
PRIMER_RIGHT_8_PENALTY=0.444466
PRIMER_LEFT_8_SEQUENCE=GAATCGACGACTTGGGTTTG
PRIMER_RIGHT_8_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_8=840,20
PRIMER_RIGHT_8=1756,20
PRIMER_LEFT_8_TM=60.495
PRIMER_RIGHT_8_TM=59.556
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=917
PRIMER_PAIR_9_PENALTY=0.939639
PRIMER_LEFT_9_PENALTY=0.495173
PRIMER_RIGHT_9_PENALTY=0.444466
PRIMER_LEFT_9_SEQUENCE=TGAATCGACGACTTGGGTTT
PRIMER_RIGHT_9_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_9=839,20
PRIMER_RIGHT_9=1756,20
PRIMER_LEFT_9_TM=60.495
PRIMER_RIGHT_9_TM=59.556
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=918
PRIMER_PAIR_10_PENALTY=0.965158
PRIMER_LEFT_10_PENALTY=0.495173
PRIMER_RIGHT_10_PENALTY=0.469985
PRIMER_LEFT_10_SEQUENCE=GAATCGACGACTTGGGTTTG
PRIMER_RIGHT_10_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_10=840,20
PRIMER_RIGHT_10=1755,20
PRIMER_LEFT_10_TM=60.495
PRIMER_RIGHT_10_TM=60.470
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=916
PRIMER_PAIR_11_PENALTY=0.965158
PRIMER_LEFT_11_PENALTY=0.495173
PRIMER_RIGHT_11_PENALTY=0.469985
PRIMER_LEFT_11_SEQUENCE=TGAATCGACGACTTGGGTTT
PRIMER_RIGHT_11_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_11=839,20
PRIMER_RIGHT_11=1755,20
PRIMER_LEFT_11_TM=60.495
PRIMER_RIGHT_11_TM=60.470
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=917
PRIMER_PAIR_12_PENALTY=1.197590
PRIMER_LEFT_12_PENALTY=0.753123
PRIMER_RIGHT_12_PENALTY=0.444466
PRIMER_LEFT_12_SEQUENCE=ACTTGGGTTTGGGCAGTTTT
PRIMER_RIGHT_12_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_12=849,20
PRIMER_RIGHT_12=1756,20
PRIMER_LEFT_12_TM=60.753
PRIMER_RIGHT_12_TM=59.556
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=908
PRIMER_PAIR_13_PENALTY=1.202993
PRIMER_LEFT_13_PENALTY=0.026532
PRIMER_RIGHT_13_PENALTY=1.176461
PRIMER_LEFT_13_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_13_SEQUENCE=CTCTAGGCGAATCACCATCA
PRIMER_LEFT_13=761,20
PRIMER_RIGHT_13=1760,20
PRIMER_LEFT_13_TM=59.973
PRIMER_RIGHT_13_TM=58.824
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=1000
PRIMER_PAIR_14_PENALTY=1.223108
PRIMER_LEFT_14_PENALTY=0.753123
PRIMER_RIGHT_14_PENALTY=0.469985
PRIMER_LEFT_14_SEQUENCE=ACTTGGGTTTGGGCAGTTTT
PRIMER_RIGHT_14_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_14=849,20
PRIMER_RIGHT_14=1755,20
PRIMER_LEFT_14_TM=60.753
PRIMER_RIGHT_14_TM=60.470
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=907
PRIMER_PAIR_15_PENALTY=1.287538
PRIMER_LEFT_15_PENALTY=0.111077
PRIMER_RIGHT_15_PENALTY=1.176461
PRIMER_LEFT_15_SEQUENCE=CTTGAATCGACGACTTGGGT
PRIMER_RIGHT_15_SEQUENCE=CTCTAGGCGAATCACCATCA
PRIMER_LEFT_15=837,20
PRIMER_RIGHT_15=1760,20
PRIMER_LEFT_15_TM=60.111
PRIMER_RIGHT_15_TM=58.824
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=924
PRIMER_PAIR_16_PENALTY=1.287538
PRIMER_LEFT_16_PENALTY=0.111077
PRIMER_RIGHT_16_PENALTY=1.176461
PRIMER_LEFT_16_SEQUENCE=ACTTGAATCGACGACTTGGG
PRIMER_RIGHT_16_SEQUENCE=CTCTAGGCGAATCACCATCA
PRIMER_LEFT_16=836,20
PRIMER_RIGHT_16=1760,20
PRIMER_LEFT_16_TM=60.111
PRIMER_RIGHT_16_TM=58.824
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=10.0000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=925
PRIMER_PAIR_17_PENALTY=1.318059
PRIMER_LEFT_17_PENALTY=0.026532
PRIMER_RIGHT_17_PENALTY=1.291527
PRIMER_LEFT_17_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_17_SEQUENCE=TCTAGGCGAATCACCATCACT
PRIMER_LEFT_17=761,20
PRIMER_RIGHT_17=1759,21
PRIMER_LEFT_17_TM=59.973
PRIMER_RIGHT_17_TM=59.708
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=999
PRIMER_PAIR_18_PENALTY=1.318059
PRIMER_LEFT_18_PENALTY=0.026532
PRIMER_RIGHT_18_PENALTY=1.291527
PRIMER_LEFT_18_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_18_SEQUENCE=CTCTAGGCGAATCACCATCAC
PRIMER_LEFT_18=761,20
PRIMER_RIGHT_18=1760,21
PRIMER_LEFT_18_TM=59.973
PRIMER_RIGHT_18_TM=59.708
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=6.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=1000
PRIMER_PAIR_19_PENALTY=1.354603
PRIMER_LEFT_19_PENALTY=0.026532
PRIMER_RIGHT_19_PENALTY=1.328071
PRIMER_LEFT_19_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_19_SEQUENCE=TCTAGGCGAATCACCATCAC
PRIMER_LEFT_19=761,20
PRIMER_RIGHT_19=1759,20
PRIMER_LEFT_19_TM=59.973
PRIMER_RIGHT_19_TM=58.672
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=999
PRIMER_PAIR_20_PENALTY=1.402604
PRIMER_LEFT_20_PENALTY=0.111077
PRIMER_RIGHT_20_PENALTY=1.291527
PRIMER_LEFT_20_SEQUENCE=CTTGAATCGACGACTTGGGT
PRIMER_RIGHT_20_SEQUENCE=TCTAGGCGAATCACCATCACT
PRIMER_LEFT_20=837,20
PRIMER_RIGHT_20=1759,21
PRIMER_LEFT_20_TM=60.111
PRIMER_RIGHT_20_TM=59.708
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=923
PRIMER_PAIR_21_PENALTY=1.402604
PRIMER_LEFT_21_PENALTY=0.111077
PRIMER_RIGHT_21_PENALTY=1.291527
PRIMER_LEFT_21_SEQUENCE=CTTGAATCGACGACTTGGGT
PRIMER_RIGHT_21_SEQUENCE=CTCTAGGCGAATCACCATCAC
PRIMER_LEFT_21=837,20
PRIMER_RIGHT_21=1760,21
PRIMER_LEFT_21_TM=60.111
PRIMER_RIGHT_21_TM=59.708
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=6.3000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=924
PRIMER_PAIR_22_PENALTY=1.402604
PRIMER_LEFT_22_PENALTY=0.111077
PRIMER_RIGHT_22_PENALTY=1.291527
PRIMER_LEFT_22_SEQUENCE=ACTTGAATCGACGACTTGGG
PRIMER_RIGHT_22_SEQUENCE=TCTAGGCGAATCACCATCACT
PRIMER_LEFT_22=836,20
PRIMER_RIGHT_22=1759,21
PRIMER_LEFT_22_TM=60.111
PRIMER_RIGHT_22_TM=59.708
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=10.0000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=924
PRIMER_PAIR_23_PENALTY=1.402604
PRIMER_LEFT_23_PENALTY=0.111077
PRIMER_RIGHT_23_PENALTY=1.291527
PRIMER_LEFT_23_SEQUENCE=ACTTGAATCGACGACTTGGG
PRIMER_RIGHT_23_SEQUENCE=CTCTAGGCGAATCACCATCAC
PRIMER_LEFT_23=836,20
PRIMER_RIGHT_23=1760,21
PRIMER_LEFT_23_TM=60.111
PRIMER_RIGHT_23_TM=59.708
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.0000
PRIMER_RIGHT_23_END_STABILITY=6.3000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=925
PRIMER_PAIR_24_PENALTY=1.420083
PRIMER_LEFT_24_PENALTY=0.975617
PRIMER_RIGHT_24_PENALTY=0.444466
PRIMER_LEFT_24_SEQUENCE=TTGCACCACTCCAATTGTTT
PRIMER_RIGHT_24_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_24=771,20
PRIMER_RIGHT_24=1756,20
PRIMER_LEFT_24_TM=59.024
PRIMER_RIGHT_24_TM=59.556
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=986
PRIMER_PAIR_25_PENALTY=1.439148
PRIMER_LEFT_25_PENALTY=0.111077
PRIMER_RIGHT_25_PENALTY=1.328071
PRIMER_LEFT_25_SEQUENCE=CTTGAATCGACGACTTGGGT
PRIMER_RIGHT_25_SEQUENCE=TCTAGGCGAATCACCATCAC
PRIMER_LEFT_25=837,20
PRIMER_RIGHT_25=1759,20
PRIMER_LEFT_25_TM=60.111
PRIMER_RIGHT_25_TM=58.672
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=6.3000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=923
PRIMER_PAIR_26_PENALTY=1.439148
PRIMER_LEFT_26_PENALTY=0.111077
PRIMER_RIGHT_26_PENALTY=1.328071
PRIMER_LEFT_26_SEQUENCE=ACTTGAATCGACGACTTGGG
PRIMER_RIGHT_26_SEQUENCE=TCTAGGCGAATCACCATCAC
PRIMER_LEFT_26=836,20
PRIMER_RIGHT_26=1759,20
PRIMER_LEFT_26_TM=60.111
PRIMER_RIGHT_26_TM=58.672
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.0000
PRIMER_RIGHT_26_END_STABILITY=6.3000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=924
PRIMER_PAIR_27_PENALTY=1.443499
PRIMER_LEFT_27_PENALTY=0.026532
PRIMER_RIGHT_27_PENALTY=1.416967
PRIMER_LEFT_27_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_27_SEQUENCE=TAGGCGAATCACCATCACTTT
PRIMER_LEFT_27=761,20
PRIMER_RIGHT_27=1757,21
PRIMER_LEFT_27_TM=59.973
PRIMER_RIGHT_27_TM=59.583
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=997
PRIMER_PAIR_28_PENALTY=1.445602
PRIMER_LEFT_28_PENALTY=0.975617
PRIMER_RIGHT_28_PENALTY=0.469985
PRIMER_LEFT_28_SEQUENCE=TTGCACCACTCCAATTGTTT
PRIMER_RIGHT_28_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_28=771,20
PRIMER_RIGHT_28=1755,20
PRIMER_LEFT_28_TM=59.024
PRIMER_RIGHT_28_TM=60.470
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=985
PRIMER_PAIR_29_PENALTY=1.528044
PRIMER_LEFT_29_PENALTY=0.111077
PRIMER_RIGHT_29_PENALTY=1.416967
PRIMER_LEFT_29_SEQUENCE=CTTGAATCGACGACTTGGGT
PRIMER_RIGHT_29_SEQUENCE=TAGGCGAATCACCATCACTTT
PRIMER_LEFT_29=837,20
PRIMER_RIGHT_29=1757,21
PRIMER_LEFT_29_TM=60.111
PRIMER_RIGHT_29_TM=59.583
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=921
PRIMER_PAIR_30_PENALTY=1.528044
PRIMER_LEFT_30_PENALTY=0.111077
PRIMER_RIGHT_30_PENALTY=1.416967
PRIMER_LEFT_30_SEQUENCE=ACTTGAATCGACGACTTGGG
PRIMER_RIGHT_30_SEQUENCE=TAGGCGAATCACCATCACTTT
PRIMER_LEFT_30=836,20
PRIMER_RIGHT_30=1757,21
PRIMER_LEFT_30_TM=60.111
PRIMER_RIGHT_30_TM=59.583
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=10.0000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=922
PRIMER_PAIR_31_PENALTY=1.562099
PRIMER_LEFT_31_PENALTY=1.117633
PRIMER_RIGHT_31_PENALTY=0.444466
PRIMER_LEFT_31_SEQUENCE=TACTTGAATCGACGACTTGGG
PRIMER_RIGHT_31_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_31=835,21
PRIMER_RIGHT_31=1756,20
PRIMER_LEFT_31_TM=60.118
PRIMER_RIGHT_31_TM=59.556
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=10.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=922
PRIMER_PAIR_32_PENALTY=1.587618
PRIMER_LEFT_32_PENALTY=1.117633
PRIMER_RIGHT_32_PENALTY=0.469985
PRIMER_LEFT_32_SEQUENCE=TACTTGAATCGACGACTTGGG
PRIMER_RIGHT_32_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_32=835,21
PRIMER_RIGHT_32=1755,20
PRIMER_LEFT_32_TM=60.118
PRIMER_RIGHT_32_TM=60.470
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=10.0000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=921
PRIMER_PAIR_33_PENALTY=1.629883
PRIMER_LEFT_33_PENALTY=0.453422
PRIMER_RIGHT_33_PENALTY=1.176461
PRIMER_LEFT_33_SEQUENCE=TGCACCACTCCAATTGTTTC
PRIMER_RIGHT_33_SEQUENCE=CTCTAGGCGAATCACCATCA
PRIMER_LEFT_33=772,20
PRIMER_RIGHT_33=1760,20
PRIMER_LEFT_33_TM=59.547
PRIMER_RIGHT_33_TM=58.824
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=989
PRIMER_PAIR_34_PENALTY=1.671633
PRIMER_LEFT_34_PENALTY=0.495173
PRIMER_RIGHT_34_PENALTY=1.176461
PRIMER_LEFT_34_SEQUENCE=GAATCGACGACTTGGGTTTG
PRIMER_RIGHT_34_SEQUENCE=CTCTAGGCGAATCACCATCA
PRIMER_LEFT_34=840,20
PRIMER_RIGHT_34=1760,20
PRIMER_LEFT_34_TM=60.495
PRIMER_RIGHT_34_TM=58.824
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=921
PRIMER_PAIR_35_PENALTY=1.671633
PRIMER_LEFT_35_PENALTY=0.495173
PRIMER_RIGHT_35_PENALTY=1.176461
PRIMER_LEFT_35_SEQUENCE=TGAATCGACGACTTGGGTTT
PRIMER_RIGHT_35_SEQUENCE=CTCTAGGCGAATCACCATCA
PRIMER_LEFT_35=839,20
PRIMER_RIGHT_35=1760,20
PRIMER_LEFT_35_TM=60.495
PRIMER_RIGHT_35_TM=58.824
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=922
PRIMER_PAIR_36_PENALTY=1.719739
PRIMER_LEFT_36_PENALTY=1.275272
PRIMER_RIGHT_36_PENALTY=0.444466
PRIMER_LEFT_36_SEQUENCE=GACTTGGGTTTGGGCAGTTT
PRIMER_RIGHT_36_SEQUENCE=AGGCGAATCACCATCACTTT
PRIMER_LEFT_36=848,20
PRIMER_RIGHT_36=1756,20
PRIMER_LEFT_36_TM=61.275
PRIMER_RIGHT_36_TM=59.556
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=909
PRIMER_PAIR_37_PENALTY=1.744949
PRIMER_LEFT_37_PENALTY=0.453422
PRIMER_RIGHT_37_PENALTY=1.291527
PRIMER_LEFT_37_SEQUENCE=TGCACCACTCCAATTGTTTC
PRIMER_RIGHT_37_SEQUENCE=TCTAGGCGAATCACCATCACT
PRIMER_LEFT_37=772,20
PRIMER_RIGHT_37=1759,21
PRIMER_LEFT_37_TM=59.547
PRIMER_RIGHT_37_TM=59.708
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=988
PRIMER_PAIR_38_PENALTY=1.744949
PRIMER_LEFT_38_PENALTY=0.453422
PRIMER_RIGHT_38_PENALTY=1.291527
PRIMER_LEFT_38_SEQUENCE=TGCACCACTCCAATTGTTTC
PRIMER_RIGHT_38_SEQUENCE=CTCTAGGCGAATCACCATCAC
PRIMER_LEFT_38=772,20
PRIMER_RIGHT_38=1760,21
PRIMER_LEFT_38_TM=59.547
PRIMER_RIGHT_38_TM=59.708
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=989
PRIMER_PAIR_39_PENALTY=1.745257
PRIMER_LEFT_39_PENALTY=1.275272
PRIMER_RIGHT_39_PENALTY=0.469985
PRIMER_LEFT_39_SEQUENCE=GACTTGGGTTTGGGCAGTTT
PRIMER_RIGHT_39_SEQUENCE=GGCGAATCACCATCACTTTC
PRIMER_LEFT_39=848,20
PRIMER_RIGHT_39=1755,20
PRIMER_LEFT_39_TM=61.275
PRIMER_RIGHT_39_TM=60.470
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=908
PRIMER_PAIR_40_PENALTY=1.781493
PRIMER_LEFT_40_PENALTY=0.453422
PRIMER_RIGHT_40_PENALTY=1.328071
PRIMER_LEFT_40_SEQUENCE=TGCACCACTCCAATTGTTTC
PRIMER_RIGHT_40_SEQUENCE=TCTAGGCGAATCACCATCAC
PRIMER_LEFT_40=772,20
PRIMER_RIGHT_40=1759,20
PRIMER_LEFT_40_TM=59.547
PRIMER_RIGHT_40_TM=58.672
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.3000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=988
PRIMER_PAIR_41_PENALTY=1.786700
PRIMER_LEFT_41_PENALTY=0.495173
PRIMER_RIGHT_41_PENALTY=1.291527
PRIMER_LEFT_41_SEQUENCE=GAATCGACGACTTGGGTTTG
PRIMER_RIGHT_41_SEQUENCE=TCTAGGCGAATCACCATCACT
PRIMER_LEFT_41=840,20
PRIMER_RIGHT_41=1759,21
PRIMER_LEFT_41_TM=60.495
PRIMER_RIGHT_41_TM=59.708
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=920
PRIMER_PAIR_42_PENALTY=1.786700
PRIMER_LEFT_42_PENALTY=0.495173
PRIMER_RIGHT_42_PENALTY=1.291527
PRIMER_LEFT_42_SEQUENCE=GAATCGACGACTTGGGTTTG
PRIMER_RIGHT_42_SEQUENCE=CTCTAGGCGAATCACCATCAC
PRIMER_LEFT_42=840,20
PRIMER_RIGHT_42=1760,21
PRIMER_LEFT_42_TM=60.495
PRIMER_RIGHT_42_TM=59.708
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=921
PRIMER_PAIR_43_PENALTY=1.786700
PRIMER_LEFT_43_PENALTY=0.495173
PRIMER_RIGHT_43_PENALTY=1.291527
PRIMER_LEFT_43_SEQUENCE=TGAATCGACGACTTGGGTTT
PRIMER_RIGHT_43_SEQUENCE=TCTAGGCGAATCACCATCACT
PRIMER_LEFT_43=839,20
PRIMER_RIGHT_43=1759,21
PRIMER_LEFT_43_TM=60.495
PRIMER_RIGHT_43_TM=59.708
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=921
PRIMER_PAIR_44_PENALTY=1.786700
PRIMER_LEFT_44_PENALTY=0.495173
PRIMER_RIGHT_44_PENALTY=1.291527
PRIMER_LEFT_44_SEQUENCE=TGAATCGACGACTTGGGTTT
PRIMER_RIGHT_44_SEQUENCE=CTCTAGGCGAATCACCATCAC
PRIMER_LEFT_44=839,20
PRIMER_RIGHT_44=1760,21
PRIMER_LEFT_44_TM=60.495
PRIMER_RIGHT_44_TM=59.708
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.3000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=922
PRIMER_PAIR_45_PENALTY=1.818069
PRIMER_LEFT_45_PENALTY=0.026532
PRIMER_RIGHT_45_PENALTY=1.791537
PRIMER_LEFT_45_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_45_SEQUENCE=CTAGGCGAATCACCATCACTT
PRIMER_LEFT_45=761,20
PRIMER_RIGHT_45=1758,21
PRIMER_LEFT_45_TM=59.973
PRIMER_RIGHT_45_TM=59.208
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=998
PRIMER_PAIR_46_PENALTY=1.823244
PRIMER_LEFT_46_PENALTY=0.495173
PRIMER_RIGHT_46_PENALTY=1.328071
PRIMER_LEFT_46_SEQUENCE=GAATCGACGACTTGGGTTTG
PRIMER_RIGHT_46_SEQUENCE=TCTAGGCGAATCACCATCAC
PRIMER_LEFT_46=840,20
PRIMER_RIGHT_46=1759,20
PRIMER_LEFT_46_TM=60.495
PRIMER_RIGHT_46_TM=58.672
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.3000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=920
PRIMER_PAIR_47_PENALTY=1.823244
PRIMER_LEFT_47_PENALTY=0.495173
PRIMER_RIGHT_47_PENALTY=1.328071
PRIMER_LEFT_47_SEQUENCE=TGAATCGACGACTTGGGTTT
PRIMER_RIGHT_47_SEQUENCE=TCTAGGCGAATCACCATCAC
PRIMER_LEFT_47=839,20
PRIMER_RIGHT_47=1759,20
PRIMER_LEFT_47_TM=60.495
PRIMER_RIGHT_47_TM=58.672
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=921
PRIMER_PAIR_48_PENALTY=1.838317
PRIMER_LEFT_48_PENALTY=0.026532
PRIMER_RIGHT_48_PENALTY=1.811785
PRIMER_LEFT_48_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_48_SEQUENCE=GCGAATCACCATCACTTTCTT
PRIMER_LEFT_48=761,20
PRIMER_RIGHT_48=1754,21
PRIMER_LEFT_48_TM=59.973
PRIMER_RIGHT_48_TM=59.188
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=994
PRIMER_PAIR_49_PENALTY=1.853469
PRIMER_LEFT_49_PENALTY=0.026532
PRIMER_RIGHT_49_PENALTY=1.826937
PRIMER_LEFT_49_SEQUENCE=GGTTTCTGGATTGCACCACT
PRIMER_RIGHT_49_SEQUENCE=TAGGCGAATCACCATCACTT
PRIMER_LEFT_49=761,20
PRIMER_RIGHT_49=1757,20
PRIMER_LEFT_49_TM=59.973
PRIMER_RIGHT_49_TM=58.173
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=997
=
SEQUENCE_ID=MAGIC.105_5_112634_112881_hi-0_ler-0_112855_2391_TRUE
SEQUENCE_TARGET=950,347
SEQUENCE_TEMPLATE=ACAAACACCGAGTATTAAGCATCCTTAAGCACGAATCATAAAGACCTGAAGGAACCGAAAAACACCAAAAACACGGGAACTTCTTCTCCTTACTCGGCATCGCCACTAAATGAATCTCCTCATCTCCGAGCATAACCACTGCAGTCTGTATTCACAAAATCAAACTAGTGAATCACTCCTAACAATTGGAAAACATCCAAAATCAGCTAAATNTCATTTCGGAAATCTCAAACCTACCTTCAATTCATGGAAACAGACTGCGTGTAGATGCATTAACTCTTGAGATTTNACCGGAGCCGATGACTCAAGCTTGCATCGAACGGCAAAAGAAGCAATCGTCTGAAGAATCGCGAGCGGAGGACATCTCTCACCAGCCGGAGATAGATGGTGGATTCGAATCTCATCGTTNGGGAATCGAAATTCATGACTTGAAGATACATGGTTCACATCAAGTTCCCCCAAACGTAAATCTCCGTGATAAACCACGGATTTATGACCCAAACGATTCATCGTGAATTCGTTCAATTCACACTTCTGAAGGACGAATCAGGTAGTGAAGATAAACAGTNTCTGGGAAAATGCGTGAGAGAATCTGCAGATTCAATTTCTCTCAAACTTCGCTACTTTTCTCTGTGATTTTTCTCCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNATGAGAACCAAAGTAGGCTCAGATATATATTTATAGGCGAGAGANGCGTTTTATTGTTACGACANCGNTTTTAATGTAAATTACGAGAAAACCTGAGACTTCTTTACACGTGGCNTCAGATGCATNATCCTGTTTTCACCAACATCGCTTGTCAGCCGTCAATTACCGTTCCTAACAAAATAAAATATTCNAGAATTATTTTTTGTNTGTATGACGAAATCGGATTAGGTCGATAATTTTGGTTGACTTCGAACAAATGGATAGTTAATGTTTACANCTATATATTGTAAAATGTGGAAACGTATTCAATAACTTACTTTAGANGTAAATAAAAATTGTGNAAAAAANACGGAAGAGGATATATATATGGTAAAATCACAAAAATCAAATAAGAACATAAAGTTGGTTGGCAAAAAAAGAGAAGACATCAAGTTGCGAGTCTTGTGACGTTTGAGTAAGAAAGGGACAAATCAATTTTATANNATNTGTATCATCNNNTTANTANGGGTGTNTTGGNAAANNAGTCANTTTTNAAAANATAATTNTGNANTTNGGCANGTTACTATTTANNANNNNNNNNNNNGGTNANTCACTTTNGNNGTNTAATAGACNAGCGNNNNNNNNNNNNNNGGNGTNGGGTNAGTGANGTGTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAATGNGNGTNTNAGGAGAGAGNNANGTTGAATCTAAAAGCACANATNNNAATTGNTTTCAACNANANTNGNGAAGAAGAANATTAGTGNCATNNNNNNNNNNNATAGAGTTNNTATGNNNTATATTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_11956570_11956866_ws-0_tsu-0_102046_607_TRUE
SEQUENCE_TARGET=950,396
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACGTTCTATAATTTNTATNNCTTTNAATAATTTATTNAATTAATACTTAAATAATAAAGGGTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTNAANNANATAAATGGTATGTCTTTCACTTTGNACGGAGTATCCCNATGTGGGGTACATAAATATTAAGAGAAAAAAAGAGATCAAGCTTACTAGTTTAAGCGTTAATAATTATACTGACTGACCAATCTACTTGTTCATGTAGCTCTAATGTTATTAATTANTATATAGTCTTAGATTGATCTAATTAATGTCTGTGTATGATGTCACTTTGTGATGATGGAAGTCATGAGAGCTTCCATTACTTAACTCTCTCTCTCTCTNTCTATNTATNTTTACGGTCATGTATAGTGTAATCANNAATAACAAGAAAAAGCCAAGTTCAAAGCACNATACTATTAGTTAAAGACAAATGCGATAATGATACGATTCTGAGCATTACTTGAGTCNCAATATATAGTCACACCCTCATCAAATTCTTTNTATCTTACCAACTTCTNAAAAAAAANNAAAAANNNAAAAGACAAGTAAATACATANATAAATATACACNCACACACACACACATATATTGATATACTTATAATNATAAATCATGGTAAAGTTTGANACAAAACTATGGAAGGCTGTGCTTATGATGTCCATGATCAACATTGGTTTAAGTGTTGTAAACGTTATGTTCAAGAAGATGATTGATGAAGGACTTAACCGTATGGTCGCGACCACTTACCGACTTGCTGTAGGAACTCTGTTCTTNATACCATTCGCGATTTTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNTTNNNNNNNNTNTNNNNNGNTNNNTGTNTTNTTNNTTNTNNNNNTTNNANTTNTNNTTNAANTNTNNNNTTNAATNATAANNGGATGTCTTGTTTATACNANTNTCTAGTTGAATGTTGTTTTCAAACTCTTTTCTCCTGTGAATATGTNATTATATATTCGTATTCCATATGAAAGATTTTGNNNNNNNNNNNNNTTGTTCTTTTTTTNNNNNTCTCTTTTNGTAGACATAACAGGCCAAAACTCACGGGTAGGATCTTGTGTTCACTCTTCTTTAGCGCTCTTCTGGGGTATGTTCATTTTATTAGACTGTTCANTTATAATAAATGTTTACAAATAAGTTTTGAGTTGTTTNTTNTGATCTTGATCGTTNNTATATTTGCAGGACAAGTTTGGTGCAATACTTCTTTCTTATTGGACTAGAGTATACATCTTCAACNTTTTCCTTAGCNTTTAGCAACATGGTTCCTTCNGTTACNTTTGCNTTGGCTCTCGTCTTTAGGTANTNAAAAATCGATCAAACNCATTTGNATTCTTCATATATATNCTCTAGTATTATCNNAATNNTTNATAAACTANANGANTAGGCAAGAGACGCTGAACATCAAGAGCAACGTAGGAAGAGCCAAACTGCTAGGCACAATGATATGTATTTGTGGAGCTTTAGTGCTTACGCTTTATAAAGGAACCGCTTTAAGTCGAGAACANAGTACTCATATGNAAACACATACAAGAACTGATTCCACCGGTGCAATGACNCAAAAGTGGGCAATGGGTTCGATCATGCTGGTTATATCNATCATNATATGGTCATCTTGGTTCATTGTTCAAGCTAAAATAAGCNGANTTTATCCNTGCCAATACACGAGTACCACGATCCTCTCTTTCTTTGGCGTGATNCAATCTGCTTTGTTGAGTTTGATCTCGGAGAGGAGCACNTCTATGTGGGTCGTTAAAGACAAGTTCCAAGTTTTAGCTTTACTTTATTCGGTGAGTNA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.301346
PRIMER_LEFT_0_PENALTY=2.276218
PRIMER_RIGHT_0_PENALTY=0.025128
PRIMER_LEFT_0_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_0_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_0=831,22
PRIMER_RIGHT_0=1590,20
PRIMER_LEFT_0_TM=60.276
PRIMER_RIGHT_0_TM=59.975
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=760
PRIMER_PAIR_1_PENALTY=2.542654
PRIMER_LEFT_1_PENALTY=2.276218
PRIMER_RIGHT_1_PENALTY=0.266437
PRIMER_LEFT_1_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_1_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_1=831,22
PRIMER_RIGHT_1=1695,20
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=865
PRIMER_PAIR_2_PENALTY=2.551967
PRIMER_LEFT_2_PENALTY=2.276218
PRIMER_RIGHT_2_PENALTY=0.275749
PRIMER_LEFT_2_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_2_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_2=831,22
PRIMER_RIGHT_2=1587,20
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=59.724
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=757
PRIMER_PAIR_3_PENALTY=2.605286
PRIMER_LEFT_3_PENALTY=2.580157
PRIMER_RIGHT_3_PENALTY=0.025128
PRIMER_LEFT_3_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_3_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_3=723,22
PRIMER_RIGHT_3=1590,20
PRIMER_LEFT_3_TM=59.420
PRIMER_RIGHT_3_TM=59.975
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=868
PRIMER_PAIR_4_PENALTY=2.846594
PRIMER_LEFT_4_PENALTY=2.580157
PRIMER_RIGHT_4_PENALTY=0.266437
PRIMER_LEFT_4_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_4_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_4=723,22
PRIMER_RIGHT_4=1695,20
PRIMER_LEFT_4_TM=59.420
PRIMER_RIGHT_4_TM=59.734
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=973
PRIMER_PAIR_5_PENALTY=2.855907
PRIMER_LEFT_5_PENALTY=2.580157
PRIMER_RIGHT_5_PENALTY=0.275749
PRIMER_LEFT_5_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_5_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_5=723,22
PRIMER_RIGHT_5=1587,20
PRIMER_LEFT_5_TM=59.420
PRIMER_RIGHT_5_TM=59.724
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=865
PRIMER_PAIR_6_PENALTY=2.870827
PRIMER_LEFT_6_PENALTY=2.845698
PRIMER_RIGHT_6_PENALTY=0.025128
PRIMER_LEFT_6_SEQUENCE=AAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_6_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_6=833,20
PRIMER_RIGHT_6=1590,20
PRIMER_LEFT_6_TM=57.154
PRIMER_RIGHT_6_TM=59.975
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=758
PRIMER_PAIR_7_PENALTY=3.112135
PRIMER_LEFT_7_PENALTY=2.845698
PRIMER_RIGHT_7_PENALTY=0.266437
PRIMER_LEFT_7_SEQUENCE=AAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_7_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_7=833,20
PRIMER_RIGHT_7=1695,20
PRIMER_LEFT_7_TM=57.154
PRIMER_RIGHT_7_TM=59.734
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=863
PRIMER_PAIR_8_PENALTY=3.121447
PRIMER_LEFT_8_PENALTY=2.845698
PRIMER_RIGHT_8_PENALTY=0.275749
PRIMER_LEFT_8_SEQUENCE=AAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_8_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_8=833,20
PRIMER_RIGHT_8=1587,20
PRIMER_LEFT_8_TM=57.154
PRIMER_RIGHT_8_TM=59.724
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=755
PRIMER_PAIR_9_PENALTY=3.151411
PRIMER_LEFT_9_PENALTY=3.126283
PRIMER_RIGHT_9_PENALTY=0.025128
PRIMER_LEFT_9_SEQUENCE=TGTGATGATGGAAGTCATGAGAG
PRIMER_RIGHT_9_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_9=734,23
PRIMER_RIGHT_9=1590,20
PRIMER_LEFT_9_TM=60.126
PRIMER_RIGHT_9_TM=59.975
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=857
PRIMER_PAIR_10_PENALTY=3.367457
PRIMER_LEFT_10_PENALTY=2.276218
PRIMER_RIGHT_10_PENALTY=1.091240
PRIMER_LEFT_10_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_10_SEQUENCE=ATCCTACCCGTGAGTTTTGG
PRIMER_LEFT_10=831,22
PRIMER_RIGHT_10=1554,20
PRIMER_LEFT_10_TM=60.276
PRIMER_RIGHT_10_TM=58.909
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.8000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=724
PRIMER_PAIR_11_PENALTY=3.392720
PRIMER_LEFT_11_PENALTY=3.126283
PRIMER_RIGHT_11_PENALTY=0.266437
PRIMER_LEFT_11_SEQUENCE=TGTGATGATGGAAGTCATGAGAG
PRIMER_RIGHT_11_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_11=734,23
PRIMER_RIGHT_11=1695,20
PRIMER_LEFT_11_TM=60.126
PRIMER_RIGHT_11_TM=59.734
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=962
PRIMER_PAIR_12_PENALTY=3.402032
PRIMER_LEFT_12_PENALTY=3.126283
PRIMER_RIGHT_12_PENALTY=0.275749
PRIMER_LEFT_12_SEQUENCE=TGTGATGATGGAAGTCATGAGAG
PRIMER_RIGHT_12_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_12=734,23
PRIMER_RIGHT_12=1587,20
PRIMER_LEFT_12_TM=60.126
PRIMER_RIGHT_12_TM=59.724
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=854
PRIMER_PAIR_13_PENALTY=3.402246
PRIMER_LEFT_13_PENALTY=3.377118
PRIMER_RIGHT_13_PENALTY=0.025128
PRIMER_LEFT_13_SEQUENCE=CACTTTGTGATGATGGAAGTCA
PRIMER_RIGHT_13_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_13=729,22
PRIMER_RIGHT_13=1590,20
PRIMER_LEFT_13_TM=58.623
PRIMER_RIGHT_13_TM=59.975
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=862
PRIMER_PAIR_14_PENALTY=3.402246
PRIMER_LEFT_14_PENALTY=3.377118
PRIMER_RIGHT_14_PENALTY=0.025128
PRIMER_LEFT_14_SEQUENCE=TGTCACTTTGTGATGATGGAAG
PRIMER_RIGHT_14_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_14=726,22
PRIMER_RIGHT_14=1590,20
PRIMER_LEFT_14_TM=58.623
PRIMER_RIGHT_14_TM=59.975
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.3000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=865
PRIMER_PAIR_15_PENALTY=3.506592
PRIMER_LEFT_15_PENALTY=2.276218
PRIMER_RIGHT_15_PENALTY=1.230374
PRIMER_LEFT_15_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_15_SEQUENCE=ATACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_15=831,22
PRIMER_RIGHT_15=1591,21
PRIMER_LEFT_15_TM=60.276
PRIMER_RIGHT_15_TM=60.230
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=6.3000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=761
PRIMER_PAIR_16_PENALTY=3.609281
PRIMER_LEFT_16_PENALTY=3.584153
PRIMER_RIGHT_16_PENALTY=0.025128
PRIMER_LEFT_16_SEQUENCE=GATGTCACTTTGTGATGATGGA
PRIMER_RIGHT_16_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_16=724,22
PRIMER_RIGHT_16=1590,20
PRIMER_LEFT_16_TM=58.416
PRIMER_RIGHT_16_TM=59.975
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=867
PRIMER_PAIR_17_PENALTY=3.643555
PRIMER_LEFT_17_PENALTY=3.377118
PRIMER_RIGHT_17_PENALTY=0.266437
PRIMER_LEFT_17_SEQUENCE=CACTTTGTGATGATGGAAGTCA
PRIMER_RIGHT_17_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_17=729,22
PRIMER_RIGHT_17=1695,20
PRIMER_LEFT_17_TM=58.623
PRIMER_RIGHT_17_TM=59.734
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=8.8000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=967
PRIMER_PAIR_18_PENALTY=3.643555
PRIMER_LEFT_18_PENALTY=3.377118
PRIMER_RIGHT_18_PENALTY=0.266437
PRIMER_LEFT_18_SEQUENCE=TGTCACTTTGTGATGATGGAAG
PRIMER_RIGHT_18_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_18=726,22
PRIMER_RIGHT_18=1695,20
PRIMER_LEFT_18_TM=58.623
PRIMER_RIGHT_18_TM=59.734
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.8000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=970
PRIMER_PAIR_19_PENALTY=3.652867
PRIMER_LEFT_19_PENALTY=3.377118
PRIMER_RIGHT_19_PENALTY=0.275749
PRIMER_LEFT_19_SEQUENCE=CACTTTGTGATGATGGAAGTCA
PRIMER_RIGHT_19_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_19=729,22
PRIMER_RIGHT_19=1587,20
PRIMER_LEFT_19_TM=58.623
PRIMER_RIGHT_19_TM=59.724
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=859
PRIMER_PAIR_20_PENALTY=3.652867
PRIMER_LEFT_20_PENALTY=3.377118
PRIMER_RIGHT_20_PENALTY=0.275749
PRIMER_LEFT_20_SEQUENCE=TGTCACTTTGTGATGATGGAAG
PRIMER_RIGHT_20_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_20=726,22
PRIMER_RIGHT_20=1587,20
PRIMER_LEFT_20_TM=58.623
PRIMER_RIGHT_20_TM=59.724
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=862
PRIMER_PAIR_21_PENALTY=3.653336
PRIMER_LEFT_21_PENALTY=2.276218
PRIMER_RIGHT_21_PENALTY=1.377118
PRIMER_LEFT_21_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_21_SEQUENCE=ATTGCACCAAACTTGTCCTG
PRIMER_LEFT_21=831,22
PRIMER_RIGHT_21=1699,20
PRIMER_LEFT_21_TM=60.276
PRIMER_RIGHT_21_TM=58.623
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=869
PRIMER_PAIR_22_PENALTY=3.671397
PRIMER_LEFT_22_PENALTY=2.580157
PRIMER_RIGHT_22_PENALTY=1.091240
PRIMER_LEFT_22_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_22_SEQUENCE=ATCCTACCCGTGAGTTTTGG
PRIMER_LEFT_22=723,22
PRIMER_RIGHT_22=1554,20
PRIMER_LEFT_22_TM=59.420
PRIMER_RIGHT_22_TM=58.909
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=8.8000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=832
PRIMER_PAIR_23_PENALTY=3.810531
PRIMER_LEFT_23_PENALTY=2.580157
PRIMER_RIGHT_23_PENALTY=1.230374
PRIMER_LEFT_23_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_23_SEQUENCE=ATACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_23=723,22
PRIMER_RIGHT_23=1591,21
PRIMER_LEFT_23_TM=59.420
PRIMER_RIGHT_23_TM=60.230
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=6.3000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=869
PRIMER_PAIR_24_PENALTY=3.813123
PRIMER_LEFT_24_PENALTY=2.276218
PRIMER_RIGHT_24_PENALTY=1.536905
PRIMER_LEFT_24_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_24_SEQUENCE=AACACAAGATCCTACCCGTGA
PRIMER_LEFT_24=831,22
PRIMER_RIGHT_24=1562,21
PRIMER_LEFT_24_TM=60.276
PRIMER_RIGHT_24_TM=59.463
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=732
PRIMER_PAIR_25_PENALTY=3.816261
PRIMER_LEFT_25_PENALTY=2.276218
PRIMER_RIGHT_25_PENALTY=1.540043
PRIMER_LEFT_25_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_25_SEQUENCE=CGTGAGTTTTGGCCTGTTATG
PRIMER_LEFT_25=831,22
PRIMER_RIGHT_25=1546,21
PRIMER_LEFT_25_TM=60.276
PRIMER_RIGHT_25_TM=60.540
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.2000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=716
PRIMER_PAIR_26_PENALTY=3.850590
PRIMER_LEFT_26_PENALTY=3.584153
PRIMER_RIGHT_26_PENALTY=0.266437
PRIMER_LEFT_26_SEQUENCE=GATGTCACTTTGTGATGATGGA
PRIMER_RIGHT_26_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_26=724,22
PRIMER_RIGHT_26=1695,20
PRIMER_LEFT_26_TM=58.416
PRIMER_RIGHT_26_TM=59.734
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.1000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=972
PRIMER_PAIR_27_PENALTY=3.859902
PRIMER_LEFT_27_PENALTY=3.584153
PRIMER_RIGHT_27_PENALTY=0.275749
PRIMER_LEFT_27_SEQUENCE=GATGTCACTTTGTGATGATGGA
PRIMER_RIGHT_27_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_27=724,22
PRIMER_RIGHT_27=1587,20
PRIMER_LEFT_27_TM=58.416
PRIMER_RIGHT_27_TM=59.724
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=864
PRIMER_PAIR_28_PENALTY=3.904243
PRIMER_LEFT_28_PENALTY=2.276218
PRIMER_RIGHT_28_PENALTY=1.628025
PRIMER_LEFT_28_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_28_SEQUENCE=CCCAGAAGAGCGCTAAAGAAG
PRIMER_LEFT_28=831,22
PRIMER_RIGHT_28=1587,21
PRIMER_LEFT_28_TM=60.276
PRIMER_RIGHT_28_TM=60.628
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=757
PRIMER_PAIR_29_PENALTY=3.936938
PRIMER_LEFT_29_PENALTY=2.845698
PRIMER_RIGHT_29_PENALTY=1.091240
PRIMER_LEFT_29_SEQUENCE=AAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_29_SEQUENCE=ATCCTACCCGTGAGTTTTGG
PRIMER_LEFT_29=833,20
PRIMER_RIGHT_29=1554,20
PRIMER_LEFT_29_TM=57.154
PRIMER_RIGHT_29_TM=58.909
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=722
PRIMER_PAIR_30_PENALTY=3.957276
PRIMER_LEFT_30_PENALTY=2.580157
PRIMER_RIGHT_30_PENALTY=1.377118
PRIMER_LEFT_30_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_30_SEQUENCE=ATTGCACCAAACTTGTCCTG
PRIMER_LEFT_30=723,22
PRIMER_RIGHT_30=1699,20
PRIMER_LEFT_30_TM=59.420
PRIMER_RIGHT_30_TM=58.623
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=977
PRIMER_PAIR_31_PENALTY=3.991785
PRIMER_LEFT_31_PENALTY=3.972018
PRIMER_RIGHT_31_PENALTY=0.019767
PRIMER_LEFT_31_SEQUENCE=GTCACACCCTCATCAAATTCTT
PRIMER_RIGHT_31_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_31=921,22
PRIMER_RIGHT_31=1902,20
PRIMER_LEFT_31_TM=58.028
PRIMER_RIGHT_31_TM=60.020
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=982
PRIMER_PAIR_32_PENALTY=3.997146
PRIMER_LEFT_32_PENALTY=3.972018
PRIMER_RIGHT_32_PENALTY=0.025128
PRIMER_LEFT_32_SEQUENCE=GTCACACCCTCATCAAATTCTT
PRIMER_RIGHT_32_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_32=921,22
PRIMER_RIGHT_32=1590,20
PRIMER_LEFT_32_TM=58.028
PRIMER_RIGHT_32_TM=59.975
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=670
PRIMER_PAIR_33_PENALTY=4.004549
PRIMER_LEFT_33_PENALTY=2.276218
PRIMER_RIGHT_33_PENALTY=1.728332
PRIMER_LEFT_33_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_33_SEQUENCE=GATCCTACCCGTGAGTTTTGG
PRIMER_LEFT_33=831,22
PRIMER_RIGHT_33=1555,21
PRIMER_LEFT_33_TM=60.276
PRIMER_RIGHT_33_TM=60.728
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=8.8000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=725
PRIMER_PAIR_34_PENALTY=4.076072
PRIMER_LEFT_34_PENALTY=2.845698
PRIMER_RIGHT_34_PENALTY=1.230374
PRIMER_LEFT_34_SEQUENCE=AAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_34_SEQUENCE=ATACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_34=833,20
PRIMER_RIGHT_34=1591,21
PRIMER_LEFT_34_TM=57.154
PRIMER_RIGHT_34_TM=60.230
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=6.3000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=759
PRIMER_PAIR_35_PENALTY=4.117063
PRIMER_LEFT_35_PENALTY=2.580157
PRIMER_RIGHT_35_PENALTY=1.536905
PRIMER_LEFT_35_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_35_SEQUENCE=AACACAAGATCCTACCCGTGA
PRIMER_LEFT_35=723,22
PRIMER_RIGHT_35=1562,21
PRIMER_LEFT_35_TM=59.420
PRIMER_RIGHT_35_TM=59.463
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=8.4000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=840
PRIMER_PAIR_36_PENALTY=4.120201
PRIMER_LEFT_36_PENALTY=2.580157
PRIMER_RIGHT_36_PENALTY=1.540043
PRIMER_LEFT_36_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_36_SEQUENCE=CGTGAGTTTTGGCCTGTTATG
PRIMER_LEFT_36=723,22
PRIMER_RIGHT_36=1546,21
PRIMER_LEFT_36_TM=59.420
PRIMER_RIGHT_36_TM=60.540
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=6.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=824
PRIMER_PAIR_37_PENALTY=4.154252
PRIMER_LEFT_37_PENALTY=2.276218
PRIMER_RIGHT_37_PENALTY=1.878034
PRIMER_LEFT_37_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_37_SEQUENCE=CCCCAGAAGAGCGCTAAAGA
PRIMER_LEFT_37=831,22
PRIMER_RIGHT_37=1588,20
PRIMER_LEFT_37_TM=60.276
PRIMER_RIGHT_37_TM=61.878
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=758
PRIMER_PAIR_38_PENALTY=4.208183
PRIMER_LEFT_38_PENALTY=2.580157
PRIMER_RIGHT_38_PENALTY=1.628025
PRIMER_LEFT_38_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_38_SEQUENCE=CCCAGAAGAGCGCTAAAGAAG
PRIMER_LEFT_38=723,22
PRIMER_RIGHT_38=1587,21
PRIMER_LEFT_38_TM=59.420
PRIMER_RIGHT_38_TM=60.628
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=865
PRIMER_PAIR_39_PENALTY=4.217523
PRIMER_LEFT_39_PENALTY=3.126283
PRIMER_RIGHT_39_PENALTY=1.091240
PRIMER_LEFT_39_SEQUENCE=TGTGATGATGGAAGTCATGAGAG
PRIMER_RIGHT_39_SEQUENCE=ATCCTACCCGTGAGTTTTGG
PRIMER_LEFT_39=734,23
PRIMER_RIGHT_39=1554,20
PRIMER_LEFT_39_TM=60.126
PRIMER_RIGHT_39_TM=58.909
PRIMER_LEFT_39_GC_PERCENT=43.478
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=8.8000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=821
PRIMER_PAIR_40_PENALTY=4.222816
PRIMER_LEFT_40_PENALTY=2.845698
PRIMER_RIGHT_40_PENALTY=1.377118
PRIMER_LEFT_40_SEQUENCE=AAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_40_SEQUENCE=ATTGCACCAAACTTGTCCTG
PRIMER_LEFT_40=833,20
PRIMER_RIGHT_40=1699,20
PRIMER_LEFT_40_TM=57.154
PRIMER_RIGHT_40_TM=58.623
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=867
PRIMER_PAIR_41_PENALTY=4.238455
PRIMER_LEFT_41_PENALTY=3.972018
PRIMER_RIGHT_41_PENALTY=0.266437
PRIMER_LEFT_41_SEQUENCE=GTCACACCCTCATCAAATTCTT
PRIMER_RIGHT_41_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_41=921,22
PRIMER_RIGHT_41=1695,20
PRIMER_LEFT_41_TM=58.028
PRIMER_RIGHT_41_TM=59.734
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=8.8000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=775
PRIMER_PAIR_42_PENALTY=4.264746
PRIMER_LEFT_42_PENALTY=4.239618
PRIMER_RIGHT_42_PENALTY=0.025128
PRIMER_LEFT_42_SEQUENCE=TGTCACTTTGTGATGATGGA
PRIMER_RIGHT_42_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_42=726,20
PRIMER_RIGHT_42=1590,20
PRIMER_LEFT_42_TM=55.760
PRIMER_RIGHT_42_TM=59.975
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.1000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=865
PRIMER_PAIR_43_PENALTY=4.269118
PRIMER_LEFT_43_PENALTY=2.276218
PRIMER_RIGHT_43_PENALTY=1.992900
PRIMER_LEFT_43_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_43_SEQUENCE=CCAGAAGAGCGCTAAAGAAGA
PRIMER_LEFT_43=831,22
PRIMER_RIGHT_43=1586,21
PRIMER_LEFT_43_TM=60.276
PRIMER_RIGHT_43_TM=59.007
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=756
PRIMER_PAIR_44_PENALTY=4.276279
PRIMER_LEFT_44_PENALTY=2.276218
PRIMER_RIGHT_44_PENALTY=2.000061
PRIMER_LEFT_44_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_44_SEQUENCE=ACACAAGATCCTACCCGTGA
PRIMER_LEFT_44=831,22
PRIMER_RIGHT_44=1561,20
PRIMER_LEFT_44_TM=60.276
PRIMER_RIGHT_44_TM=58.000
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=731
PRIMER_PAIR_45_PENALTY=4.308489
PRIMER_LEFT_45_PENALTY=2.580157
PRIMER_RIGHT_45_PENALTY=1.728332
PRIMER_LEFT_45_SEQUENCE=TGATGTCACTTTGTGATGATGG
PRIMER_RIGHT_45_SEQUENCE=GATCCTACCCGTGAGTTTTGG
PRIMER_LEFT_45=723,22
PRIMER_RIGHT_45=1555,21
PRIMER_LEFT_45_TM=59.420
PRIMER_RIGHT_45_TM=60.728
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=833
PRIMER_PAIR_46_PENALTY=4.348770
PRIMER_LEFT_46_PENALTY=2.276218
PRIMER_RIGHT_46_PENALTY=2.072552
PRIMER_LEFT_46_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_46_SEQUENCE=CCAGAAGAGCGCTAAAGAAGAG
PRIMER_LEFT_46=831,22
PRIMER_RIGHT_46=1586,22
PRIMER_LEFT_46_TM=60.276
PRIMER_RIGHT_46_TM=59.927
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=756
PRIMER_PAIR_47_PENALTY=4.356657
PRIMER_LEFT_47_PENALTY=3.126283
PRIMER_RIGHT_47_PENALTY=1.230374
PRIMER_LEFT_47_SEQUENCE=TGTGATGATGGAAGTCATGAGAG
PRIMER_RIGHT_47_SEQUENCE=ATACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_47=734,23
PRIMER_RIGHT_47=1591,21
PRIMER_LEFT_47_TM=60.126
PRIMER_RIGHT_47_TM=60.230
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=858
PRIMER_PAIR_48_PENALTY=4.375691
PRIMER_LEFT_48_PENALTY=3.972018
PRIMER_RIGHT_48_PENALTY=0.403673
PRIMER_LEFT_48_SEQUENCE=GTCACACCCTCATCAAATTCTT
PRIMER_RIGHT_48_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_48=921,22
PRIMER_RIGHT_48=1911,20
PRIMER_LEFT_48_TM=58.028
PRIMER_RIGHT_48_TM=59.596
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=991
PRIMER_PAIR_49_PENALTY=4.382604
PRIMER_LEFT_49_PENALTY=2.845698
PRIMER_RIGHT_49_PENALTY=1.536905
PRIMER_LEFT_49_SEQUENCE=AAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_49_SEQUENCE=AACACAAGATCCTACCCGTGA
PRIMER_LEFT_49=833,20
PRIMER_RIGHT_49=1562,21
PRIMER_LEFT_49_TM=57.154
PRIMER_RIGHT_49_TM=59.463
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=730
=
SEQUENCE_ID=MAGIC.426_3_11672353_11673005_wil-2_zu-0_39012_119356_TRUE
SEQUENCE_TARGET=950,752
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNATNGAGATNGANCGTTNGNAGTTTTATAAACAATTTNAAAAACNAAAAAAANTAAANNCAATGTNTTTTTTATTAATTCACAAACAATAATAAATCATAGGATCGAATATTTACACGGTATCAAAANCTACTCGNCGCTANTATATAAAAANTNAAGTCAAATATCAACCGCAATTATTANACCAGCAAGACAATAATTCATAAACTTAATATAAACANAAATAAATTAATGTTACANAACNATATNTGGNGAGGNTTATTACTATCTTCNTCCTCTNAANACACATCTCCTAACCTTAAGCTTTAGACATGACCAAAACATTTTTCTCCTTCTCCTTCTTCTTCACCANCTCTCTNCTCCTTCTCCTCGCGGCAACTTCCGCAACCGCATCTACAGGGAATGTTACTAGTGGGTTGAGATATGACGGNTGTGCTCCTGGAGACACCGTTGGAGAATGTATAACCGCANCGGTTGAGNAGGAGGATGAGGAAGGAGTTGAGGCGGTNGTNCGGCGGATTCTACAACAAANAAAGTANTTAAGCTACAAAACTTTGCAGAAGCAGCCNACTTGTGATGGTAGAATNGCTGGTAATTGCATAGGNACTGTTAATCCAAAAGGTGCAACTTGTACTTACTACCAGAGATGCAAACGTGCNGCATAGTTATTTATCTTCGTGTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATNTTNTGGTGTCAACCNAGTTTCTCTCNNNNTTTNTNATTTTTAGATAAAAATCANCCGNTTTTNNANCTANATTTTGANNTNAATTGTTCANAATGCAAAGAAACNTTNATCAATNAAATTTATTANCTTTATTACNCCTTNCTAATTCCCNTTTTTTANTTGAAATCGGTANTNAAANAAGAANAACTATTTNCACANNTTNAACAGAAGTCTTCATACTTTTGGACTNGTTAGATACCATNCAACCAAANTANATACAACCTAAGTNAANAAAGAANCATGCAAGATGTTNCNTTATTGTNCATANTGCATCNNCTTGTNGTTTTAANATAAACAATTTTAACTATACTAGGGNAGAATTCGGGNTACGCCNANAAANATTTGATATTTTAAAGTTTGTTAATTTTGNANAAACAAAAAACATNTAATGTTGTATTTAACATTTTACTAAAGAAACCATATATATAATACTATTAATGGTTTNACTCTATCTATTTNTTNTTATTTCANTGTTATTTATATTTANTTTAATNATTGCTTATTTTTATCATTNATANGGGCATAGAATTATTGATNTGAGATTATTTATGTTCTATAAACAATCTCCCCTNATAGNACCANTGATTTTCTNAAATGTATTAAAATCCTACNTTNTTGAATTTACCATTTGTTAAAATCAATCCAAATATATGGTTATTTGATTAATNATTTANAAAAATGTGCAAAANTCGGNTTTTGTAGTAAAATTANATANGNTATG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_16031868_16032147_ct-1_mt-0_10538_93378_TRUE
SEQUENCE_TARGET=950,379
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_5665127_5665838_hi-0_mt-0_3475301_266_TRUE
SEQUENCE_TARGET=950,811
SEQUENCE_TEMPLATE=TAAAAAAGGATTAGCAGAAGAGAGATCAGAGAGATGCCACGTGGAGCCTACGGCCAATGGGAGAGCGATAAGCTTATCAAAGATTTCAAAGTGCGGAGGGTAAAGTGGGCGACGTTGCTTTTCCACGTGNCAATNTNTCATTGGTANGAAANGTTAAAAGTTAGATTTCCGCTTAGTCGGTATTTNGGTCCCATACTGTCNTCGCAACTTGGNCCTCTCTCTTGCGNTCAGAAGGTTTTGTCTTTTNAGTTTCTTCTTGATTTTGNTTTTATCCTTTGTTATTTTTAGTCTGGTCCGTNTTTTCTCCGTATCTTGTTTCAAATACATTNTCTCGTAAAGAAACCGCNTTTTTTGGTTTAGTACTGAATGATAAAAGTATTGTTTTCNTACGAAATTTANGTCAACCGNCCTAGTNCTAGAGGATCACCTAGACCATATGCATGGGACTTNGTCGAGCTTCGATGTGGATTAGCCCNAGTTATCAATAGAATACTGAAAATTGGCNNNNNTAACTTTGATTTAATGGAAATGCNTTTTTATANNATACTNAAAATTTAANAGANATGATGATNGATGAGTAGTAAATTTCAAATGGTATAACAAGTTTAATTGTTTTTTTAGTAAGTTATATATACTGANCAAGACGTTTTTGTTTGAGTCAGTGACTATCNCTCTTTGTTTTTACTCAACTTAATTCCATANATCCTGGTACTTATAACGTGTACAATCTAGAATCACAAGCGTTTAGAGATAGTGATAATTAGCTTGGCTACTTTCTCAGCTGTGTTGTTGCTTGATCGANGNNGGAAAATGANAGTGGNCATATTGAAGTTCTTGCTTAGNGCTATTGNTAATAGAACGGTTNGAGAGCCGGCCAAGCTTATGGATAATATATTTATANATGGAGAATGAGAATAACATAAAGATGAGANATNGTTGAACCTAATTAATNAAAAAAACAATTTACATGAANTTTTTTTCTTTANAAGATTTCANTCTGNAACATGCATAGAAACTTGTCTCATNACCCTCGTAGTCNTATAGCAAGCATTAGTTTTATGATAGAGATGTTTCNGTTACGAAAGAAANTTTAAACCAACTTAAGTGGTTGTAAATCACGTTGTANTTTTTATGTTTATGTACAANTNCAAATTACAAATCTTCNTTTTTAATTATAATCTTTGCCACTGAAATTCACGATAATATCACTAAGGTTCGATGCAATTCGTCAAACATNTCACGTACTAAAATTTGTTNGGCGANNGANGGAGACATAGATGTAAATGGAAACGTNNACTCGTAAATTGTGTCATCTTTCGTGGCCCATNAANTNGAGATGGAGTTTTATCGTTTTCTATATCNNNNNNNNNNNNNNNNNNNNNNAANAAGGTTTTATTATAAAAAGTACCAAAGAAATGGACCGTAGTCACCATNNNNNNNNTTACATGGTTACATGGCGCGACACGTAGTGTCACTTAATGCTGCAAGTGTTTTTTCCTCTTTGTTTTTTGCCTCCCTGAATATTTTAAATTTGNTATATAAAATNTTTCTGCTGACGAGTTTTAGTTACTCTAGTTTCTAAAATGGTCTAGAAAAAGTTGTGNTATATAANTATAATAACTCGGTAANGGCTACGTACAGAGTCAGCCACGTAACANTAAATGGATGTGTCATGGACCCGTATTACACTCNNCATTATACACGNNTNNNGNNTANATNNNNTANNNNNNGATNANNNANNTTTNNNNTNTCAANNNNGANANANNTANTNNNGTANNNNNTNTTCNAAANNCATAGTNANTNTANNTNATNANAAATANGTNTTNANTNGTTTNNAAACNANTNNNCANNNNNNNANTTATTNTTNTNNCNNNNANTNANTNTGANTNNTTTNNNANNNGNAANTANCTATNTNNNNNNTGNNNNNNNAANNNNNNTTNTTTNNTNAANANTANNTNTNATTNTNNANNNTACANNNANNCCACAACTAGAAAGACGANTCCAAAAACNAAAGNAAATAGGAANTACTCGACCTTTNTTTTCTAACNTCAATCCACCACATNATATTTGTNGTTTTTAGCGNAATAATTNAGTTCTAACACACCACATGATTTGCTAAGCTACCAGTACTACTTTCTTTGTTAGGCCNTGAGAAATTGAATATTCCTTCCTCTTATTCTGCATATTCTTTGTATTTGTTTTGTTACCTACCTCTTCTAGTGTGTAGCCTACGTAAATATACACTCAAGTTGTTTTGATGTAACCNGTTTTCTCCACCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACACTACATATCAATTCNTACTTATTATAANTACACTNATTANATNTNAATGCATGAANNNAAACTCGAATTCGATATNNTNCGATTCGTCAAAAAANNNNNNNNNNNNNNNNNNTGGTATCAATTAGAGGTCTGAATTTGTTCAATATCGTCCACAATTCCCTACCTAAAAAATTAGAGCTTTTTGGACCAAATCCAAAAATGGATCTAAAAAGTGAAAACCAAAATATTCCTTTAAGAATCTACACAGATTCGAACCAAACCGGATTAAACCGNATAGTCNACGGCACGTGTGGAAACGCGNNNCGTTTCAAAAACATTTGGGGTANTNNNNNNNNNNNNNNNNNNNNNNNNATCAGGGTTTTAGGAGAAACCCAGGAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14067733_14068151_ler-0_zu-0_56709_11089_TRUE
SEQUENCE_TARGET=950,518
SEQUENCE_TEMPLATE=GGCTGGCTACTTTCTAGCNNNNNNNNNNNNNNNNNNNNNNNATGGCCGGATCTTTNCTAGCGGTTCGTTTAAATCAGGTTATTAGTACCGTTTTTATGTTTTTCCNTTTCNATAATCACCNTGATCTTNGTACCAGTTTCAATTTCATAAAGCNTATGTGTTTCAATTTTAATCTCNTTTTTNGGGTTTTAATCATCGTTNGTTTAAACCCAATTNAAANTTCTCAATCNTTTGATTTNGAATCTCAATCCAGCTTATCTNGAAAGAATTTAATATCAAGANAGATCATGATTTCTCNTTTTTCAAAGATNGTTGNTGNTNTNGAGGAATATAGATCAAACTACAAACTTNAAAGTNTNAATTTCTATTTTCTTCTTAAGATTCAATCNTTGGTTAGTTTNTTGGAGAACATCAATCAAGGAGATGATATTANGGAGCAAAGNNGCGGATTCACAAGAGTGATCCTACCTGCAACTCTCAAGAGAGAGGCAGTGCACATAGTCGTCACGACANCCAACAAATATCCGGCCACCGATCATCACTGGTGAAGAGAAAATGTCTGCCTGCAACTCCATNCTCGNGATTTCTCCTACTTTTGANTCATGTGAATCTCTGCTGAGANTGGNGGNTCTCACACGAAGGACATGAACTCTTCCCGAGCTTGAGCATACAGTCACCAACCTGGAAAATACATGGAAACANAAGGAGGAGGTACNTTCATANATCCATGATAATTAAATACATTCATATGTGCTTTTNNCTTGCAGTTAATACCTGTCTGATGCAAGCAGTTNATGCGATTCAAAATGCAGATTCTCATCAATGTATGCAGATGCTGTGATCGGGTCCCCAATATTGTNTTCCCACACAAGACACCCAGATTCCTGAAATANATTCCAAAACAAAAGTCTTGACTCATGGTACTGAAGTTTTGAGACGAGTNCCACNNNAACAACTGATCCTCATGAACGTCCCTNTCAATGTCCTTGCAGTAATAAAANTCAACCTAATATATCTTTATTCCACTGATTTTGCTACAGATTCAANTATATCTTTAGTGCATCCACAANCTGTTAATTCCAAAAGGGTATATTCCTTCTAATATAAACTCGGAAATCAATATGCCAATGAACNCNGAAGAATCTTATTAAACTGGTGTGTTTCAGAAAGGACTCCCTAGAAGATGCATAGGANGATGTATGAATTTAGAATATGCAGTGACATGATAAAGTCTATTGCACATCAGATTTCAGTCCTAAAATAACAAAAGCCCCTNGCACNAAAAAAAAAGTCGACAGCAATANATANACTATACAAGACCCATGTCCAAGGGATATAAGAAAGTACCGGTTCTAGNGAATAAACACNTCCATTTCTACNGCANACAAGCACCTGNAGAAAAATAGACNTGTAGTCAGNCTNAGNGAGANATCTCTACACAAAAAGGATATACTGCAGATGTACTCAGGAATCAAGGGCACCTGAGAAGGAAGCACATGTGACATGCAAGGTCCAGCAAATATTGGTCCACCAGTCCTATACTGCATTAATGTAACCGCTGACTTAGTTTGAGCACTGTGAGATGGCCAAAACAAANTGACAGGAACAAAAGATGTGGAACGCAATGTGGACTTTATGATATCATTTGAATGAACTGGGATCAATTCATACACATACCNTCCAANTAATAGTTCCAGACGGACNCATTGCGATCACTTGGCCATCAACTAAGCAGCANATAACTGTGACAGAAAATAGAGANTAACAATGANNNTNAAATCAAGGAAATNGAAANAAANTAGTNGAGNAAGAANATACCGNTTNGANTTGNNGGGGTAATGCATAGGGAACCAAAAATCGGTGCCTCTANTTCAANTAGCCATAAGGTGTGGAATGGGGNATCCTGCATTTGCAAAATNAACCNGAATAAANAAGGNTCCNTCATTACTTTATAAGAAGTCAACNACAAATCTTCCTCTGGAGCCAAGAAAAANTGTAATGCTGATCAGNTAAGTTAAACAGCTCTATAGGGTAAGNCATACANGACCCAATTTTGGTAGTAACCATTATTTCTAANGACTATTCNGCTTCATTNNTGTAGAGCANANANNTTCATTTTCGACAACTGACCTATTCTTATCATGACGACNTACTGGAATGCAAAANGTGAGGCAACAAACGGTATNCNAAAGGTATACAAGCAACAACANATAGGGTGTTAAGAGTANGGANAAAAGNNNCATACCTTTATNGATACAGCTATCACGCGACCACTGGTAGAGGCCACGTAAAGTGAACTATGACCCTGGGTGAAGCATCAAAGATAAATCAGGTGATGTNAAGAGAACTNCAATAACCAACAAAATCTTTGACCACAGANGACATTGTTNAGCTGTGGANAAGCAACTATGCCTAACC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.809493
PRIMER_LEFT_0_PENALTY=0.801031
PRIMER_RIGHT_0_PENALTY=0.008461
PRIMER_LEFT_0_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_0_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_0=825,20
PRIMER_RIGHT_0=1586,20
PRIMER_LEFT_0_TM=60.801
PRIMER_RIGHT_0_TM=59.992
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.8000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=762
PRIMER_PAIR_1_PENALTY=0.971434
PRIMER_LEFT_1_PENALTY=0.962973
PRIMER_RIGHT_1_PENALTY=0.008461
PRIMER_LEFT_1_SEQUENCE=CCTGTCTGATGCAAGCAGTT
PRIMER_RIGHT_1_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_1=773,20
PRIMER_RIGHT_1=1586,20
PRIMER_LEFT_1_TM=59.037
PRIMER_RIGHT_1_TM=59.992
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=814
PRIMER_PAIR_2_PENALTY=1.383829
PRIMER_LEFT_2_PENALTY=1.375368
PRIMER_RIGHT_2_PENALTY=0.008461
PRIMER_LEFT_2_SEQUENCE=CCGAGCTTGAGCATACAGTC
PRIMER_RIGHT_2_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_2=655,20
PRIMER_RIGHT_2=1586,20
PRIMER_LEFT_2_TM=58.625
PRIMER_RIGHT_2_TM=59.992
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=932
PRIMER_PAIR_3_PENALTY=1.391454
PRIMER_LEFT_3_PENALTY=0.962973
PRIMER_RIGHT_3_PENALTY=0.428481
PRIMER_LEFT_3_SEQUENCE=CCTGTCTGATGCAAGCAGTT
PRIMER_RIGHT_3_SEQUENCE=CACATTGCGTTCCACATCTT
PRIMER_LEFT_3=773,20
PRIMER_RIGHT_3=1628,20
PRIMER_LEFT_3_TM=59.037
PRIMER_RIGHT_3_TM=59.572
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=856
PRIMER_PAIR_4_PENALTY=1.423576
PRIMER_LEFT_4_PENALTY=1.415114
PRIMER_RIGHT_4_PENALTY=0.008461
PRIMER_LEFT_4_SEQUENCE=CACACAAGACACCCAGATTCC
PRIMER_RIGHT_4_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_4=864,21
PRIMER_RIGHT_4=1586,20
PRIMER_LEFT_4_TM=60.415
PRIMER_RIGHT_4_TM=59.992
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=723
PRIMER_PAIR_5_PENALTY=1.463932
PRIMER_LEFT_5_PENALTY=0.262854
PRIMER_RIGHT_5_PENALTY=1.201078
PRIMER_LEFT_5_SEQUENCE=AAATGTCTGCCTGCAACTCC
PRIMER_RIGHT_5_SEQUENCE=GGACCAATATTTGCTGGACCT
PRIMER_LEFT_5=553,20
PRIMER_RIGHT_5=1528,21
PRIMER_LEFT_5_TM=60.263
PRIMER_RIGHT_5_TM=60.201
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=976
PRIMER_PAIR_6_PENALTY=1.482967
PRIMER_LEFT_6_PENALTY=1.474506
PRIMER_RIGHT_6_PENALTY=0.008461
PRIMER_LEFT_6_SEQUENCE=TCCCGAGCTTGAGCATACAG
PRIMER_RIGHT_6_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_6=653,20
PRIMER_RIGHT_6=1586,20
PRIMER_LEFT_6_TM=61.475
PRIMER_RIGHT_6_TM=59.992
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=934
PRIMER_PAIR_7_PENALTY=1.601810
PRIMER_LEFT_7_PENALTY=1.593348
PRIMER_RIGHT_7_PENALTY=0.008461
PRIMER_LEFT_7_SEQUENCE=CCACACAAGACACCCAGATT
PRIMER_RIGHT_7_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_7=863,20
PRIMER_RIGHT_7=1586,20
PRIMER_LEFT_7_TM=58.407
PRIMER_RIGHT_7_TM=59.992
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=724
PRIMER_PAIR_8_PENALTY=1.627304
PRIMER_LEFT_8_PENALTY=0.801031
PRIMER_RIGHT_8_PENALTY=0.826272
PRIMER_LEFT_8_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_8_SEQUENCE=TGCGTTCCACATCTTTTGTT
PRIMER_LEFT_8=825,20
PRIMER_RIGHT_8=1623,20
PRIMER_LEFT_8_TM=60.801
PRIMER_RIGHT_8_TM=59.174
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.8000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=799
PRIMER_PAIR_9_PENALTY=1.627304
PRIMER_LEFT_9_PENALTY=0.801031
PRIMER_RIGHT_9_PENALTY=0.826272
PRIMER_LEFT_9_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_9_SEQUENCE=TTGCGTTCCACATCTTTTGT
PRIMER_LEFT_9=825,20
PRIMER_RIGHT_9=1624,20
PRIMER_LEFT_9_TM=60.801
PRIMER_RIGHT_9_TM=59.174
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.8000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=800
PRIMER_PAIR_10_PENALTY=1.684090
PRIMER_LEFT_10_PENALTY=1.375368
PRIMER_RIGHT_10_PENALTY=0.308722
PRIMER_LEFT_10_SEQUENCE=CCGAGCTTGAGCATACAGTC
PRIMER_RIGHT_10_SEQUENCE=TTGTTTTGGCCATCTCACAG
PRIMER_LEFT_10=655,20
PRIMER_RIGHT_10=1594,20
PRIMER_LEFT_10_TM=58.625
PRIMER_RIGHT_10_TM=59.691
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=940
PRIMER_PAIR_11_PENALTY=1.723836
PRIMER_LEFT_11_PENALTY=1.415114
PRIMER_RIGHT_11_PENALTY=0.308722
PRIMER_LEFT_11_SEQUENCE=CACACAAGACACCCAGATTCC
PRIMER_RIGHT_11_SEQUENCE=TTGTTTTGGCCATCTCACAG
PRIMER_LEFT_11=864,21
PRIMER_RIGHT_11=1594,20
PRIMER_LEFT_11_TM=60.415
PRIMER_RIGHT_11_TM=59.691
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=731
PRIMER_PAIR_12_PENALTY=1.766624
PRIMER_LEFT_12_PENALTY=0.801031
PRIMER_RIGHT_12_PENALTY=0.965592
PRIMER_LEFT_12_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_12_SEQUENCE=AAGTCCACATTGCGTTCCAC
PRIMER_LEFT_12=825,20
PRIMER_RIGHT_12=1633,20
PRIMER_LEFT_12_TM=60.801
PRIMER_RIGHT_12_TM=60.966
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.8000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=809
PRIMER_PAIR_13_PENALTY=1.769995
PRIMER_LEFT_13_PENALTY=1.415114
PRIMER_RIGHT_13_PENALTY=0.354881
PRIMER_LEFT_13_SEQUENCE=CACACAAGACACCCAGATTCC
PRIMER_RIGHT_13_SEQUENCE=TGGTTCCCTATGCATTACCC
PRIMER_LEFT_13=864,21
PRIMER_RIGHT_13=1852,20
PRIMER_LEFT_13_TM=60.415
PRIMER_RIGHT_13_TM=59.645
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=989
PRIMER_PAIR_14_PENALTY=1.783227
PRIMER_LEFT_14_PENALTY=1.474506
PRIMER_RIGHT_14_PENALTY=0.308722
PRIMER_LEFT_14_SEQUENCE=TCCCGAGCTTGAGCATACAG
PRIMER_RIGHT_14_SEQUENCE=TTGTTTTGGCCATCTCACAG
PRIMER_LEFT_14=653,20
PRIMER_RIGHT_14=1594,20
PRIMER_LEFT_14_TM=61.475
PRIMER_RIGHT_14_TM=59.691
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=5.7000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=942
PRIMER_PAIR_15_PENALTY=1.789245
PRIMER_LEFT_15_PENALTY=0.962973
PRIMER_RIGHT_15_PENALTY=0.826272
PRIMER_LEFT_15_SEQUENCE=CCTGTCTGATGCAAGCAGTT
PRIMER_RIGHT_15_SEQUENCE=TGCGTTCCACATCTTTTGTT
PRIMER_LEFT_15=773,20
PRIMER_RIGHT_15=1623,20
PRIMER_LEFT_15_TM=59.037
PRIMER_RIGHT_15_TM=59.174
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=851
PRIMER_PAIR_16_PENALTY=1.789245
PRIMER_LEFT_16_PENALTY=0.962973
PRIMER_RIGHT_16_PENALTY=0.826272
PRIMER_LEFT_16_SEQUENCE=CCTGTCTGATGCAAGCAGTT
PRIMER_RIGHT_16_SEQUENCE=TTGCGTTCCACATCTTTTGT
PRIMER_LEFT_16=773,20
PRIMER_RIGHT_16=1624,20
PRIMER_LEFT_16_TM=59.037
PRIMER_RIGHT_16_TM=59.174
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=852
PRIMER_PAIR_17_PENALTY=1.803849
PRIMER_LEFT_17_PENALTY=1.375368
PRIMER_RIGHT_17_PENALTY=0.428481
PRIMER_LEFT_17_SEQUENCE=CCGAGCTTGAGCATACAGTC
PRIMER_RIGHT_17_SEQUENCE=CACATTGCGTTCCACATCTT
PRIMER_LEFT_17=655,20
PRIMER_RIGHT_17=1628,20
PRIMER_LEFT_17_TM=58.625
PRIMER_RIGHT_17_TM=59.572
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=974
PRIMER_PAIR_18_PENALTY=1.813856
PRIMER_LEFT_18_PENALTY=1.415114
PRIMER_RIGHT_18_PENALTY=0.398742
PRIMER_LEFT_18_SEQUENCE=CACACAAGACACCCAGATTCC
PRIMER_RIGHT_18_SEQUENCE=GGTTCCCTATGCATTACCCC
PRIMER_LEFT_18=864,21
PRIMER_RIGHT_18=1851,20
PRIMER_LEFT_18_TM=60.415
PRIMER_RIGHT_18_TM=60.399
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=10.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=988
PRIMER_PAIR_19_PENALTY=1.843595
PRIMER_LEFT_19_PENALTY=1.415114
PRIMER_RIGHT_19_PENALTY=0.428481
PRIMER_LEFT_19_SEQUENCE=CACACAAGACACCCAGATTCC
PRIMER_RIGHT_19_SEQUENCE=CACATTGCGTTCCACATCTT
PRIMER_LEFT_19=864,21
PRIMER_RIGHT_19=1628,20
PRIMER_LEFT_19_TM=60.415
PRIMER_RIGHT_19_TM=59.572
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=765
PRIMER_PAIR_20_PENALTY=1.856088
PRIMER_LEFT_20_PENALTY=1.847627
PRIMER_RIGHT_20_PENALTY=0.008461
PRIMER_LEFT_20_SEQUENCE=TTCCCGAGCTTGAGCATACA
PRIMER_RIGHT_20_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_20=652,20
PRIMER_RIGHT_20=1586,20
PRIMER_LEFT_20_TM=61.848
PRIMER_RIGHT_20_TM=59.992
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=5.6000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=7.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=935
PRIMER_PAIR_21_PENALTY=1.902070
PRIMER_LEFT_21_PENALTY=1.593348
PRIMER_RIGHT_21_PENALTY=0.308722
PRIMER_LEFT_21_SEQUENCE=CCACACAAGACACCCAGATT
PRIMER_RIGHT_21_SEQUENCE=TTGTTTTGGCCATCTCACAG
PRIMER_LEFT_21=863,20
PRIMER_RIGHT_21=1594,20
PRIMER_LEFT_21_TM=58.407
PRIMER_RIGHT_21_TM=59.691
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=732
PRIMER_PAIR_22_PENALTY=1.902987
PRIMER_LEFT_22_PENALTY=1.474506
PRIMER_RIGHT_22_PENALTY=0.428481
PRIMER_LEFT_22_SEQUENCE=TCCCGAGCTTGAGCATACAG
PRIMER_RIGHT_22_SEQUENCE=CACATTGCGTTCCACATCTT
PRIMER_LEFT_22=653,20
PRIMER_RIGHT_22=1628,20
PRIMER_LEFT_22_TM=61.475
PRIMER_RIGHT_22_TM=59.572
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.7000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=976
PRIMER_PAIR_23_PENALTY=1.928565
PRIMER_LEFT_23_PENALTY=0.962973
PRIMER_RIGHT_23_PENALTY=0.965592
PRIMER_LEFT_23_SEQUENCE=CCTGTCTGATGCAAGCAGTT
PRIMER_RIGHT_23_SEQUENCE=AAGTCCACATTGCGTTCCAC
PRIMER_LEFT_23=773,20
PRIMER_RIGHT_23=1633,20
PRIMER_LEFT_23_TM=59.037
PRIMER_RIGHT_23_TM=60.966
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=861
PRIMER_PAIR_24_PENALTY=1.948229
PRIMER_LEFT_24_PENALTY=1.593348
PRIMER_RIGHT_24_PENALTY=0.354881
PRIMER_LEFT_24_SEQUENCE=CCACACAAGACACCCAGATT
PRIMER_RIGHT_24_SEQUENCE=TGGTTCCCTATGCATTACCC
PRIMER_LEFT_24=863,20
PRIMER_RIGHT_24=1852,20
PRIMER_LEFT_24_TM=58.407
PRIMER_RIGHT_24_TM=59.645
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=990
PRIMER_PAIR_25_PENALTY=1.978660
PRIMER_LEFT_25_PENALTY=1.970198
PRIMER_RIGHT_25_PENALTY=0.008461
PRIMER_LEFT_25_SEQUENCE=ACAGTCACCAACCTGGAAAA
PRIMER_RIGHT_25_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_25=669,20
PRIMER_RIGHT_25=1586,20
PRIMER_LEFT_25_TM=58.030
PRIMER_RIGHT_25_TM=59.992
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=7.3000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=918
PRIMER_PAIR_26_PENALTY=1.978815
PRIMER_LEFT_26_PENALTY=1.970353
PRIMER_RIGHT_26_PENALTY=0.008461
PRIMER_LEFT_26_SEQUENCE=GCATACAGTCACCAACCTGGA
PRIMER_RIGHT_26_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_26=665,21
PRIMER_RIGHT_26=1586,20
PRIMER_LEFT_26_TM=60.970
PRIMER_RIGHT_26_TM=59.992
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=7.3000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=922
PRIMER_PAIR_27_PENALTY=1.992090
PRIMER_LEFT_27_PENALTY=1.593348
PRIMER_RIGHT_27_PENALTY=0.398742
PRIMER_LEFT_27_SEQUENCE=CCACACAAGACACCCAGATT
PRIMER_RIGHT_27_SEQUENCE=GGTTCCCTATGCATTACCCC
PRIMER_LEFT_27=863,20
PRIMER_RIGHT_27=1851,20
PRIMER_LEFT_27_TM=58.407
PRIMER_RIGHT_27_TM=60.399
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.6000
PRIMER_RIGHT_27_END_STABILITY=10.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=989
PRIMER_PAIR_28_PENALTY=1.998550
PRIMER_LEFT_28_PENALTY=1.990089
PRIMER_RIGHT_28_PENALTY=0.008461
PRIMER_LEFT_28_SEQUENCE=TTCCCACACAAGACACCCAG
PRIMER_RIGHT_28_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_28=860,20
PRIMER_RIGHT_28=1586,20
PRIMER_LEFT_28_TM=61.990
PRIMER_RIGHT_28_TM=59.992
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=727
PRIMER_PAIR_29_PENALTY=2.002109
PRIMER_LEFT_29_PENALTY=0.801031
PRIMER_RIGHT_29_PENALTY=1.201078
PRIMER_LEFT_29_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_29_SEQUENCE=GGACCAATATTTGCTGGACCT
PRIMER_LEFT_29=825,20
PRIMER_RIGHT_29=1528,21
PRIMER_LEFT_29_TM=60.801
PRIMER_RIGHT_29_TM=60.201
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.8000
PRIMER_RIGHT_29_END_STABILITY=7.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=704
PRIMER_PAIR_30_PENALTY=2.011730
PRIMER_LEFT_30_PENALTY=2.003268
PRIMER_RIGHT_30_PENALTY=0.008461
PRIMER_LEFT_30_SEQUENCE=CGAAGGACATGAACTCTTCCC
PRIMER_RIGHT_30_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_30=636,21
PRIMER_RIGHT_30=1586,20
PRIMER_LEFT_30_TM=61.003
PRIMER_RIGHT_30_TM=59.992
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=7.3000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=951
PRIMER_PAIR_31_PENALTY=2.021829
PRIMER_LEFT_31_PENALTY=1.593348
PRIMER_RIGHT_31_PENALTY=0.428481
PRIMER_LEFT_31_SEQUENCE=CCACACAAGACACCCAGATT
PRIMER_RIGHT_31_SEQUENCE=CACATTGCGTTCCACATCTT
PRIMER_LEFT_31=863,20
PRIMER_RIGHT_31=1628,20
PRIMER_LEFT_31_TM=58.407
PRIMER_RIGHT_31_TM=59.572
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=766
PRIMER_PAIR_32_PENALTY=2.064636
PRIMER_LEFT_32_PENALTY=0.801031
PRIMER_RIGHT_32_PENALTY=1.263605
PRIMER_LEFT_32_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_32_SEQUENCE=GCTTAGTTGATGGCCAAGTGA
PRIMER_LEFT_32=825,20
PRIMER_RIGHT_32=1732,21
PRIMER_LEFT_32_TM=60.801
PRIMER_RIGHT_32_TM=60.264
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.8000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=908
PRIMER_PAIR_33_PENALTY=2.065873
PRIMER_LEFT_33_PENALTY=2.057411
PRIMER_RIGHT_33_PENALTY=0.008461
PRIMER_LEFT_33_SEQUENCE=AGCTTGAGCATACAGTCACCAA
PRIMER_RIGHT_33_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_33=658,22
PRIMER_RIGHT_33=1586,20
PRIMER_LEFT_33_TM=59.943
PRIMER_RIGHT_33_TM=59.992
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=929
PRIMER_PAIR_34_PENALTY=2.067998
PRIMER_LEFT_34_PENALTY=2.059537
PRIMER_RIGHT_34_PENALTY=0.008461
PRIMER_LEFT_34_SEQUENCE=GCTTGAGCATACAGTCACCAA
PRIMER_RIGHT_34_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_34=659,21
PRIMER_RIGHT_34=1586,20
PRIMER_LEFT_34_TM=58.940
PRIMER_RIGHT_34_TM=59.992
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=7.3000
PRIMER_PAIR_34_COMPL_ANY=7.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=928
PRIMER_PAIR_35_PENALTY=2.117145
PRIMER_LEFT_35_PENALTY=2.108684
PRIMER_RIGHT_35_PENALTY=0.008461
PRIMER_LEFT_35_SEQUENCE=CATACAGTCACCAACCTGGAAA
PRIMER_RIGHT_35_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_35=666,22
PRIMER_RIGHT_35=1586,20
PRIMER_LEFT_35_TM=59.891
PRIMER_RIGHT_35_TM=59.992
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=7.3000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=921
PRIMER_PAIR_36_PENALTY=2.156349
PRIMER_LEFT_36_PENALTY=1.847627
PRIMER_RIGHT_36_PENALTY=0.308722
PRIMER_LEFT_36_SEQUENCE=TTCCCGAGCTTGAGCATACA
PRIMER_RIGHT_36_SEQUENCE=TTGTTTTGGCCATCTCACAG
PRIMER_LEFT_36=652,20
PRIMER_RIGHT_36=1594,20
PRIMER_LEFT_36_TM=61.848
PRIMER_RIGHT_36_TM=59.691
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=5.6000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=943
PRIMER_PAIR_37_PENALTY=2.164051
PRIMER_LEFT_37_PENALTY=0.962973
PRIMER_RIGHT_37_PENALTY=1.201078
PRIMER_LEFT_37_SEQUENCE=CCTGTCTGATGCAAGCAGTT
PRIMER_RIGHT_37_SEQUENCE=GGACCAATATTTGCTGGACCT
PRIMER_LEFT_37=773,20
PRIMER_RIGHT_37=1528,21
PRIMER_LEFT_37_TM=59.037
PRIMER_RIGHT_37_TM=60.201
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=756
PRIMER_PAIR_38_PENALTY=2.169604
PRIMER_LEFT_38_PENALTY=0.262854
PRIMER_RIGHT_38_PENALTY=1.906750
PRIMER_LEFT_38_SEQUENCE=AAATGTCTGCCTGCAACTCC
PRIMER_RIGHT_38_SEQUENCE=CCAATATTTGCTGGACCTTG
PRIMER_LEFT_38=553,20
PRIMER_RIGHT_38=1525,20
PRIMER_LEFT_38_TM=60.263
PRIMER_RIGHT_38_TM=58.093
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=973
PRIMER_PAIR_39_PENALTY=2.176036
PRIMER_LEFT_39_PENALTY=0.801031
PRIMER_RIGHT_39_PENALTY=1.375005
PRIMER_LEFT_39_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_39_SEQUENCE=CATTGCGTTCCACATCTTTT
PRIMER_LEFT_39=825,20
PRIMER_RIGHT_39=1626,20
PRIMER_LEFT_39_TM=60.801
PRIMER_RIGHT_39_TM=58.625
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.8000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=802
PRIMER_PAIR_40_PENALTY=2.176036
PRIMER_LEFT_40_PENALTY=0.801031
PRIMER_RIGHT_40_PENALTY=1.375005
PRIMER_LEFT_40_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_40_SEQUENCE=TAAAGTCCACATTGCGTTCC
PRIMER_LEFT_40=825,20
PRIMER_RIGHT_40=1635,20
PRIMER_LEFT_40_TM=60.801
PRIMER_RIGHT_40_TM=58.625
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.8000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=811
PRIMER_PAIR_41_PENALTY=2.199369
PRIMER_LEFT_41_PENALTY=2.190908
PRIMER_RIGHT_41_PENALTY=0.008461
PRIMER_LEFT_41_SEQUENCE=GCTTGAGCATACAGTCACCAAC
PRIMER_RIGHT_41_SEQUENCE=GCCATCTCACAGTGCTCAAA
PRIMER_LEFT_41=659,22
PRIMER_RIGHT_41=1586,20
PRIMER_LEFT_41_TM=59.809
PRIMER_RIGHT_41_TM=59.992
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.3000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=928
PRIMER_PAIR_42_PENALTY=2.201640
PRIMER_LEFT_42_PENALTY=1.375368
PRIMER_RIGHT_42_PENALTY=0.826272
PRIMER_LEFT_42_SEQUENCE=CCGAGCTTGAGCATACAGTC
PRIMER_RIGHT_42_SEQUENCE=TGCGTTCCACATCTTTTGTT
PRIMER_LEFT_42=655,20
PRIMER_RIGHT_42=1623,20
PRIMER_LEFT_42_TM=58.625
PRIMER_RIGHT_42_TM=59.174
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=969
PRIMER_PAIR_43_PENALTY=2.201640
PRIMER_LEFT_43_PENALTY=1.375368
PRIMER_RIGHT_43_PENALTY=0.826272
PRIMER_LEFT_43_SEQUENCE=CCGAGCTTGAGCATACAGTC
PRIMER_RIGHT_43_SEQUENCE=TTGCGTTCCACATCTTTTGT
PRIMER_LEFT_43=655,20
PRIMER_RIGHT_43=1624,20
PRIMER_LEFT_43_TM=58.625
PRIMER_RIGHT_43_TM=59.174
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=970
PRIMER_PAIR_44_PENALTY=2.226578
PRIMER_LEFT_44_PENALTY=0.962973
PRIMER_RIGHT_44_PENALTY=1.263605
PRIMER_LEFT_44_SEQUENCE=CCTGTCTGATGCAAGCAGTT
PRIMER_RIGHT_44_SEQUENCE=GCTTAGTTGATGGCCAAGTGA
PRIMER_LEFT_44=773,20
PRIMER_RIGHT_44=1732,21
PRIMER_LEFT_44_TM=59.037
PRIMER_RIGHT_44_TM=60.264
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=960
PRIMER_PAIR_45_PENALTY=2.241387
PRIMER_LEFT_45_PENALTY=1.415114
PRIMER_RIGHT_45_PENALTY=0.826272
PRIMER_LEFT_45_SEQUENCE=CACACAAGACACCCAGATTCC
PRIMER_RIGHT_45_SEQUENCE=TGCGTTCCACATCTTTTGTT
PRIMER_LEFT_45=864,21
PRIMER_RIGHT_45=1623,20
PRIMER_LEFT_45_TM=60.415
PRIMER_RIGHT_45_TM=59.174
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=760
PRIMER_PAIR_46_PENALTY=2.241387
PRIMER_LEFT_46_PENALTY=1.415114
PRIMER_RIGHT_46_PENALTY=0.826272
PRIMER_LEFT_46_SEQUENCE=CACACAAGACACCCAGATTCC
PRIMER_RIGHT_46_SEQUENCE=TTGCGTTCCACATCTTTTGT
PRIMER_LEFT_46=864,21
PRIMER_RIGHT_46=1624,20
PRIMER_LEFT_46_TM=60.415
PRIMER_RIGHT_46_TM=59.174
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.1000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=761
PRIMER_PAIR_47_PENALTY=2.275537
PRIMER_LEFT_47_PENALTY=0.801031
PRIMER_RIGHT_47_PENALTY=1.474506
PRIMER_LEFT_47_SEQUENCE=TATGCAGATGCTGTGATCGG
PRIMER_RIGHT_47_SEQUENCE=AAAGTCCACATTGCGTTCCA
PRIMER_LEFT_47=825,20
PRIMER_RIGHT_47=1634,20
PRIMER_LEFT_47_TM=60.801
PRIMER_RIGHT_47_TM=61.475
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.8000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=810
PRIMER_PAIR_48_PENALTY=2.276108
PRIMER_LEFT_48_PENALTY=1.847627
PRIMER_RIGHT_48_PENALTY=0.428481
PRIMER_LEFT_48_SEQUENCE=TTCCCGAGCTTGAGCATACA
PRIMER_RIGHT_48_SEQUENCE=CACATTGCGTTCCACATCTT
PRIMER_LEFT_48=652,20
PRIMER_RIGHT_48=1628,20
PRIMER_LEFT_48_TM=61.848
PRIMER_RIGHT_48_TM=59.572
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.6000
PRIMER_RIGHT_48_END_STABILITY=6.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=977
PRIMER_PAIR_49_PENALTY=2.278920
PRIMER_LEFT_49_PENALTY=1.970198
PRIMER_RIGHT_49_PENALTY=0.308722
PRIMER_LEFT_49_SEQUENCE=ACAGTCACCAACCTGGAAAA
PRIMER_RIGHT_49_SEQUENCE=TTGTTTTGGCCATCTCACAG
PRIMER_LEFT_49=669,20
PRIMER_RIGHT_49=1594,20
PRIMER_LEFT_49_TM=58.030
PRIMER_RIGHT_49_TM=59.691
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=926
=
SEQUENCE_ID=MAGIC.329_1_15792655_15792733_oy-0_ws-0_100287_88195_TRUE
SEQUENCE_TARGET=950,178
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4453149_4453198_zu-0_can-0_6001_40271_TRUE
SEQUENCE_TARGET=950,149
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_9099953_9100251_ct-1_wil-2_83982_492475_TRUE
SEQUENCE_TARGET=950,398
SEQUENCE_TEMPLATE=AGATTAGGTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATTGCCAATCCAAGTAACACNCCAANCCCATANCCTATTNCCACGCCTTNCNAATTCAACACTTNTTCNTCTTCTTCTNCNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCNNNNGNNGCNTNAGTCCCAAAGCANCTTTNTTGAAGAGNNAAACCACAAANCCCNGCATTTCCTTCAAANGACGATTTAGGTTGCCCAGTTANNTGTGTTCCTTGTGGTATTTCNCCGTTGAGNTGGTTGTGAGACACGTTNATGTATGCCAAAAACGAGAGAGTCCCTANNNNNNNNNNNNNNGTCCCTGAGAGTTGNTTNCTTGATAGGTCTAGTGACTCGANNNNCTNAAGATTNGCCAAAGACANAGGAATATGNCCTGTGAAGGCGTTGTTNGATAAGTTGAGTGNGATCAGTGCCTTTAANAGACCNATNGATTCAGGAATTTCTCCTTCAAGTCTNTTTCCNGAAANATCAATGGNCGCTGANGAACTAANGACCCTNTNTTGCTCCATAGANAGACCTTTATATNGTAAATCTATAGNTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATANCATATATAGACCNTGATNTTCATTCATCGTGAGTGATGATGCTTTCCNATTCTCAAAAAANTNTNNGGGCAAGCTNCCNGTNAATTTATTACCAGCTATCTCAANNATNCGCAGCTCANNAAANCCCAGAGNNCCTTGATTNGGANGAGATANANGACCATANAANTTGTTTGAANGGAGAANAANGACTTGNANNNTCGGTAAANCCTTAANNNANAAAGGAAATGTATCTTNGATTCCGTTGTGNTCCACACTNANNAACTGTAGAGNTGAGCAATTTAGAANAGANCTTGGAAGNTTTCCNGTTNNNNGATTGTAGCCAACNTCGAGTNANCGTAGAGGTGCATCGGCATAATANNTGTCTGGAATACTTCCTTCCAAGTTGTTCTTCCGGAGATTCAANNTCAANAAGTTACTNNGACATGGNGGAATTGGACCGGTNAAGTTGTTGTAGCTTAGATNAANGNNANCAAGNNACNTTCTACTGCANATTGAAAGAGGTATGTCGCCTCNGNATCTATTGTNNNTTGCNGAGANGNAGNTGANAGANAGNGGTAGATGTGGNAGTGCTCCTTCNANANNGTTTGNCNNCAAATTTAAGATCCGTACNGATGAATTTACTAAAANTTNTGATGAACCTTCGAAGCCAGTNAACAAATNTNNNNNANTNAAANCNGNGCTCAGACGAGGAAGGCTCCATAACCANTNTNGGATTTTCCCACTGATNTTATTGNTNNNNNNGNCAATANACTCCAAATTCNGAAGGNTCTTNAAGATGTTTGGGAAANCACTGATGTTGNNNNNNNNNNNNNNNNNNNNNNNNANGGTCGATGGAATGTATGAATCCNAACTTAAACNNGCCTGAGATATCCAANCACCGGAAANATCGAGGAGCAACAAANATTTGAGAGAGGANAAGNNNNNNNNNTTAATTGGGTAGCTTGTGTTTAGGNAAGAAAGGTNGAGCTCTTTGAGGTTGATNAGCTTTGAGATAGGCTCTATGATTTTTCCTTCAAAATGGTTTTCACCTAGGTNCAAATTCTCTAGCCTTGANNNNNANGANGAGTNANGAACTTCAATNGAACCANTGAGATTGTTTCCACNTAAATNAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.107891
PRIMER_LEFT_0_PENALTY=0.754768
PRIMER_RIGHT_0_PENALTY=0.353123
PRIMER_LEFT_0_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_0_SEQUENCE=TATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_0=908,20
PRIMER_RIGHT_0=1581,20
PRIMER_LEFT_0_TM=59.245
PRIMER_RIGHT_0_TM=60.353
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=674
PRIMER_PAIR_1_PENALTY=1.218448
PRIMER_LEFT_1_PENALTY=0.754768
PRIMER_RIGHT_1_PENALTY=0.463680
PRIMER_LEFT_1_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_1_SEQUENCE=GGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_1=908,20
PRIMER_RIGHT_1=1584,20
PRIMER_LEFT_1_TM=59.245
PRIMER_RIGHT_1_TM=60.464
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=677
PRIMER_PAIR_2_PENALTY=1.868326
PRIMER_LEFT_2_PENALTY=0.754768
PRIMER_RIGHT_2_PENALTY=1.113558
PRIMER_LEFT_2_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_2_SEQUENCE=TTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_2=908,20
PRIMER_RIGHT_2=1582,20
PRIMER_LEFT_2_TM=59.245
PRIMER_RIGHT_2_TM=58.886
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=675
PRIMER_PAIR_3_PENALTY=2.011848
PRIMER_LEFT_3_PENALTY=0.754768
PRIMER_RIGHT_3_PENALTY=1.257080
PRIMER_LEFT_3_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_3_SEQUENCE=GTTATGGAGCCTTCCTCGTC
PRIMER_LEFT_3=908,20
PRIMER_RIGHT_3=1583,20
PRIMER_LEFT_3_TM=59.245
PRIMER_RIGHT_3_TM=58.743
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=676
PRIMER_PAIR_4_PENALTY=2.028012
PRIMER_LEFT_4_PENALTY=0.754768
PRIMER_RIGHT_4_PENALTY=1.273245
PRIMER_LEFT_4_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_4_SEQUENCE=GTTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_4=908,20
PRIMER_RIGHT_4=1583,21
PRIMER_LEFT_4_TM=59.245
PRIMER_RIGHT_4_TM=59.727
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=676
PRIMER_PAIR_5_PENALTY=2.482092
PRIMER_LEFT_5_PENALTY=0.375090
PRIMER_RIGHT_5_PENALTY=2.107002
PRIMER_LEFT_5_SEQUENCE=TGCCAAAAACGAGAGAGTCC
PRIMER_RIGHT_5_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_5=554,20
PRIMER_RIGHT_5=1392,20
PRIMER_LEFT_5_TM=60.375
PRIMER_RIGHT_5_TM=57.893
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=839
PRIMER_PAIR_6_PENALTY=2.557289
PRIMER_LEFT_6_PENALTY=2.204166
PRIMER_RIGHT_6_PENALTY=0.353123
PRIMER_LEFT_6_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_6_SEQUENCE=TATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_6=907,21
PRIMER_RIGHT_6=1581,20
PRIMER_LEFT_6_TM=61.204
PRIMER_RIGHT_6_TM=60.353
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=675
PRIMER_PAIR_7_PENALTY=2.667846
PRIMER_LEFT_7_PENALTY=2.204166
PRIMER_RIGHT_7_PENALTY=0.463680
PRIMER_LEFT_7_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_7_SEQUENCE=GGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_7=907,21
PRIMER_RIGHT_7=1584,20
PRIMER_LEFT_7_TM=61.204
PRIMER_RIGHT_7_TM=60.464
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=678
PRIMER_PAIR_8_PENALTY=2.861770
PRIMER_LEFT_8_PENALTY=0.754768
PRIMER_RIGHT_8_PENALTY=2.107002
PRIMER_LEFT_8_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_8_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_8=908,20
PRIMER_RIGHT_8=1392,20
PRIMER_LEFT_8_TM=59.245
PRIMER_RIGHT_8_TM=57.893
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=485
PRIMER_PAIR_9_PENALTY=3.050787
PRIMER_LEFT_9_PENALTY=2.697664
PRIMER_RIGHT_9_PENALTY=0.353123
PRIMER_LEFT_9_SEQUENCE=TCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_9_SEQUENCE=TATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_9=907,20
PRIMER_RIGHT_9=1581,20
PRIMER_LEFT_9_TM=57.302
PRIMER_RIGHT_9_TM=60.353
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=675
PRIMER_PAIR_10_PENALTY=3.051465
PRIMER_LEFT_10_PENALTY=0.944463
PRIMER_RIGHT_10_PENALTY=2.107002
PRIMER_LEFT_10_SEQUENCE=GACGATTTAGGTTGCCCAGT
PRIMER_RIGHT_10_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_10=477,20
PRIMER_RIGHT_10=1392,20
PRIMER_LEFT_10_TM=59.056
PRIMER_RIGHT_10_TM=57.893
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=916
PRIMER_PAIR_11_PENALTY=3.161344
PRIMER_LEFT_11_PENALTY=2.697664
PRIMER_RIGHT_11_PENALTY=0.463680
PRIMER_LEFT_11_SEQUENCE=TCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_11_SEQUENCE=GGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_11=907,20
PRIMER_RIGHT_11=1584,20
PRIMER_LEFT_11_TM=57.302
PRIMER_RIGHT_11_TM=60.464
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=678
PRIMER_PAIR_12_PENALTY=3.161731
PRIMER_LEFT_12_PENALTY=0.754768
PRIMER_RIGHT_12_PENALTY=2.406964
PRIMER_LEFT_12_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_12_SEQUENCE=GGATTCATACATTCCATCGACC
PRIMER_LEFT_12=908,20
PRIMER_RIGHT_12=1727,22
PRIMER_LEFT_12_TM=59.245
PRIMER_RIGHT_12_TM=60.407
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.455
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=820
PRIMER_PAIR_13_PENALTY=3.317724
PRIMER_LEFT_13_PENALTY=2.204166
PRIMER_RIGHT_13_PENALTY=1.113558
PRIMER_LEFT_13_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_13_SEQUENCE=TTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_13=907,21
PRIMER_RIGHT_13=1582,20
PRIMER_LEFT_13_TM=61.204
PRIMER_RIGHT_13_TM=58.886
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=676
PRIMER_PAIR_14_PENALTY=3.390413
PRIMER_LEFT_14_PENALTY=0.754768
PRIMER_RIGHT_14_PENALTY=2.635645
PRIMER_LEFT_14_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_14_SEQUENCE=TTATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_14=908,20
PRIMER_RIGHT_14=1582,21
PRIMER_LEFT_14_TM=59.245
PRIMER_RIGHT_14_TM=61.636
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=675
PRIMER_PAIR_15_PENALTY=3.461246
PRIMER_LEFT_15_PENALTY=2.204166
PRIMER_RIGHT_15_PENALTY=1.257080
PRIMER_LEFT_15_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_15_SEQUENCE=GTTATGGAGCCTTCCTCGTC
PRIMER_LEFT_15=907,21
PRIMER_RIGHT_15=1583,20
PRIMER_LEFT_15_TM=61.204
PRIMER_RIGHT_15_TM=58.743
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=677
PRIMER_PAIR_16_PENALTY=3.473459
PRIMER_LEFT_16_PENALTY=1.366457
PRIMER_RIGHT_16_PENALTY=2.107002
PRIMER_LEFT_16_SEQUENCE=GACGATTTAGGTTGCCCAGTT
PRIMER_RIGHT_16_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_16=477,21
PRIMER_RIGHT_16=1392,20
PRIMER_LEFT_16_TM=60.366
PRIMER_RIGHT_16_TM=57.893
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=916
PRIMER_PAIR_17_PENALTY=3.477410
PRIMER_LEFT_17_PENALTY=2.204166
PRIMER_RIGHT_17_PENALTY=1.273245
PRIMER_LEFT_17_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_17_SEQUENCE=GTTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_17=907,21
PRIMER_RIGHT_17=1583,21
PRIMER_LEFT_17_TM=61.204
PRIMER_RIGHT_17_TM=59.727
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.1000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=677
PRIMER_PAIR_18_PENALTY=3.528172
PRIMER_LEFT_18_PENALTY=1.421170
PRIMER_RIGHT_18_PENALTY=2.107002
PRIMER_LEFT_18_SEQUENCE=ACGATTTAGGTTGCCCAGTT
PRIMER_RIGHT_18_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_18=478,20
PRIMER_RIGHT_18=1392,20
PRIMER_LEFT_18_TM=58.579
PRIMER_RIGHT_18_TM=57.893
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=915
PRIMER_PAIR_19_PENALTY=3.604120
PRIMER_LEFT_19_PENALTY=3.250996
PRIMER_RIGHT_19_PENALTY=0.353123
PRIMER_LEFT_19_SEQUENCE=ATCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_19_SEQUENCE=TATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_19=906,21
PRIMER_RIGHT_19=1581,20
PRIMER_LEFT_19_TM=57.749
PRIMER_RIGHT_19_TM=60.353
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=676
PRIMER_PAIR_20_PENALTY=3.714677
PRIMER_LEFT_20_PENALTY=3.250996
PRIMER_RIGHT_20_PENALTY=0.463680
PRIMER_LEFT_20_SEQUENCE=ATCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_20_SEQUENCE=GGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_20=906,21
PRIMER_RIGHT_20=1584,20
PRIMER_LEFT_20_TM=57.749
PRIMER_RIGHT_20_TM=60.464
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=679
PRIMER_PAIR_21_PENALTY=3.731516
PRIMER_LEFT_21_PENALTY=1.624514
PRIMER_RIGHT_21_PENALTY=2.107002
PRIMER_LEFT_21_SEQUENCE=ATGCCAAAAACGAGAGAGTCC
PRIMER_RIGHT_21_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_21=553,21
PRIMER_RIGHT_21=1392,20
PRIMER_LEFT_21_TM=60.625
PRIMER_RIGHT_21_TM=57.893
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=840
PRIMER_PAIR_22_PENALTY=3.766663
PRIMER_LEFT_22_PENALTY=3.413539
PRIMER_RIGHT_22_PENALTY=0.353123
PRIMER_LEFT_22_SEQUENCE=ATCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_22_SEQUENCE=TATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_22=906,22
PRIMER_RIGHT_22=1581,20
PRIMER_LEFT_22_TM=61.414
PRIMER_RIGHT_22_TM=60.353
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=676
PRIMER_PAIR_23_PENALTY=3.811222
PRIMER_LEFT_23_PENALTY=2.697664
PRIMER_RIGHT_23_PENALTY=1.113558
PRIMER_LEFT_23_SEQUENCE=TCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_23_SEQUENCE=TTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_23=907,20
PRIMER_RIGHT_23=1582,20
PRIMER_LEFT_23_TM=57.302
PRIMER_RIGHT_23_TM=58.886
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.1000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=676
PRIMER_PAIR_24_PENALTY=3.877220
PRIMER_LEFT_24_PENALTY=3.413539
PRIMER_RIGHT_24_PENALTY=0.463680
PRIMER_LEFT_24_SEQUENCE=ATCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_24_SEQUENCE=GGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_24=906,22
PRIMER_RIGHT_24=1584,20
PRIMER_LEFT_24_TM=61.414
PRIMER_RIGHT_24_TM=60.464
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=679
PRIMER_PAIR_25_PENALTY=3.954744
PRIMER_LEFT_25_PENALTY=2.697664
PRIMER_RIGHT_25_PENALTY=1.257080
PRIMER_LEFT_25_SEQUENCE=TCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_25_SEQUENCE=GTTATGGAGCCTTCCTCGTC
PRIMER_LEFT_25=907,20
PRIMER_RIGHT_25=1583,20
PRIMER_LEFT_25_TM=57.302
PRIMER_RIGHT_25_TM=58.743
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=677
PRIMER_PAIR_26_PENALTY=3.965576
PRIMER_LEFT_26_PENALTY=0.754768
PRIMER_RIGHT_26_PENALTY=3.210808
PRIMER_LEFT_26_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_26_SEQUENCE=GGTTATGGAGCCTTCCTCGTC
PRIMER_LEFT_26=908,20
PRIMER_RIGHT_26=1584,21
PRIMER_LEFT_26_TM=59.245
PRIMER_RIGHT_26_TM=62.211
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=57.143
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=677
PRIMER_PAIR_27_PENALTY=3.970909
PRIMER_LEFT_27_PENALTY=2.697664
PRIMER_RIGHT_27_PENALTY=1.273245
PRIMER_LEFT_27_SEQUENCE=TCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_27_SEQUENCE=GTTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_27=907,20
PRIMER_RIGHT_27=1583,21
PRIMER_LEFT_27_TM=57.302
PRIMER_RIGHT_27_TM=59.727
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=8.1000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=677
PRIMER_PAIR_28_PENALTY=4.311168
PRIMER_LEFT_28_PENALTY=2.204166
PRIMER_RIGHT_28_PENALTY=2.107002
PRIMER_LEFT_28_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_28_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_28=907,21
PRIMER_RIGHT_28=1392,20
PRIMER_LEFT_28_TM=61.204
PRIMER_RIGHT_28_TM=57.893
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=486
PRIMER_PAIR_29_PENALTY=4.364555
PRIMER_LEFT_29_PENALTY=3.250996
PRIMER_RIGHT_29_PENALTY=1.113558
PRIMER_LEFT_29_SEQUENCE=ATCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_29_SEQUENCE=TTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_29=906,21
PRIMER_RIGHT_29=1582,20
PRIMER_LEFT_29_TM=57.749
PRIMER_RIGHT_29_TM=58.886
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.1000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=677
PRIMER_PAIR_30_PENALTY=4.508077
PRIMER_LEFT_30_PENALTY=3.250996
PRIMER_RIGHT_30_PENALTY=1.257080
PRIMER_LEFT_30_SEQUENCE=ATCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_30_SEQUENCE=GTTATGGAGCCTTCCTCGTC
PRIMER_LEFT_30=906,21
PRIMER_RIGHT_30=1583,20
PRIMER_LEFT_30_TM=57.749
PRIMER_RIGHT_30_TM=58.743
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=678
PRIMER_PAIR_31_PENALTY=4.524241
PRIMER_LEFT_31_PENALTY=3.250996
PRIMER_RIGHT_31_PENALTY=1.273245
PRIMER_LEFT_31_SEQUENCE=ATCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_31_SEQUENCE=GTTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_31=906,21
PRIMER_RIGHT_31=1583,21
PRIMER_LEFT_31_TM=57.749
PRIMER_RIGHT_31_TM=59.727
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=678
PRIMER_PAIR_32_PENALTY=4.527098
PRIMER_LEFT_32_PENALTY=3.413539
PRIMER_RIGHT_32_PENALTY=1.113558
PRIMER_LEFT_32_SEQUENCE=ATCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_32_SEQUENCE=TTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_32=906,22
PRIMER_RIGHT_32=1582,20
PRIMER_LEFT_32_TM=61.414
PRIMER_RIGHT_32_TM=58.886
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=677
PRIMER_PAIR_33_PENALTY=4.611129
PRIMER_LEFT_33_PENALTY=2.204166
PRIMER_RIGHT_33_PENALTY=2.406964
PRIMER_LEFT_33_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_33_SEQUENCE=GGATTCATACATTCCATCGACC
PRIMER_LEFT_33=907,21
PRIMER_RIGHT_33=1727,22
PRIMER_LEFT_33_TM=61.204
PRIMER_RIGHT_33_TM=60.407
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=821
PRIMER_PAIR_34_PENALTY=4.670620
PRIMER_LEFT_34_PENALTY=3.413539
PRIMER_RIGHT_34_PENALTY=1.257080
PRIMER_LEFT_34_SEQUENCE=ATCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_34_SEQUENCE=GTTATGGAGCCTTCCTCGTC
PRIMER_LEFT_34=906,22
PRIMER_RIGHT_34=1583,20
PRIMER_LEFT_34_TM=61.414
PRIMER_RIGHT_34_TM=58.743
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.1000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=678
PRIMER_PAIR_35_PENALTY=4.686784
PRIMER_LEFT_35_PENALTY=3.413539
PRIMER_RIGHT_35_PENALTY=1.273245
PRIMER_LEFT_35_SEQUENCE=ATCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_35_SEQUENCE=GTTATGGAGCCTTCCTCGTCT
PRIMER_LEFT_35=906,22
PRIMER_RIGHT_35=1583,21
PRIMER_LEFT_35_TM=61.414
PRIMER_RIGHT_35_TM=59.727
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=8.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=678
PRIMER_PAIR_36_PENALTY=4.691934
PRIMER_LEFT_36_PENALTY=4.338811
PRIMER_RIGHT_36_PENALTY=0.353123
PRIMER_LEFT_36_SEQUENCE=ATCGTGAGTGATGATGCTTT
PRIMER_RIGHT_36_SEQUENCE=TATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_36=906,20
PRIMER_RIGHT_36=1581,20
PRIMER_LEFT_36_TM=55.661
PRIMER_RIGHT_36_TM=60.353
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=676
PRIMER_PAIR_37_PENALTY=4.719172
PRIMER_LEFT_37_PENALTY=2.612170
PRIMER_RIGHT_37_PENALTY=2.107002
PRIMER_LEFT_37_SEQUENCE=TATGCCAAAAACGAGAGAGTCC
PRIMER_RIGHT_37_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_37=552,22
PRIMER_RIGHT_37=1392,20
PRIMER_LEFT_37_TM=60.612
PRIMER_RIGHT_37_TM=57.893
PRIMER_LEFT_37_GC_PERCENT=45.455
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=841
PRIMER_PAIR_38_PENALTY=4.802491
PRIMER_LEFT_38_PENALTY=4.338811
PRIMER_RIGHT_38_PENALTY=0.463680
PRIMER_LEFT_38_SEQUENCE=ATCGTGAGTGATGATGCTTT
PRIMER_RIGHT_38_SEQUENCE=GGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_38=906,20
PRIMER_RIGHT_38=1584,20
PRIMER_LEFT_38_TM=55.661
PRIMER_RIGHT_38_TM=60.464
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=679
PRIMER_PAIR_39_PENALTY=4.804666
PRIMER_LEFT_39_PENALTY=2.697664
PRIMER_RIGHT_39_PENALTY=2.107002
PRIMER_LEFT_39_SEQUENCE=TCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_39_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_39=907,20
PRIMER_RIGHT_39=1392,20
PRIMER_LEFT_39_TM=57.302
PRIMER_RIGHT_39_TM=57.893
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=486
PRIMER_PAIR_40_PENALTY=4.830357
PRIMER_LEFT_40_PENALTY=4.477233
PRIMER_RIGHT_40_PENALTY=0.353123
PRIMER_LEFT_40_SEQUENCE=TCAGGAATTTCTCCTTCAAGTC
PRIMER_RIGHT_40_SEQUENCE=TATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_40=726,22
PRIMER_RIGHT_40=1581,20
PRIMER_LEFT_40_TM=57.523
PRIMER_RIGHT_40_TM=60.353
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=856
PRIMER_PAIR_41_PENALTY=4.839811
PRIMER_LEFT_41_PENALTY=2.204166
PRIMER_RIGHT_41_PENALTY=2.635645
PRIMER_LEFT_41_SEQUENCE=TCGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_41_SEQUENCE=TTATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_41=907,21
PRIMER_RIGHT_41=1582,21
PRIMER_LEFT_41_TM=61.204
PRIMER_RIGHT_41_TM=61.636
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=676
PRIMER_PAIR_42_PENALTY=4.866037
PRIMER_LEFT_42_PENALTY=0.754768
PRIMER_RIGHT_42_PENALTY=4.111269
PRIMER_LEFT_42_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_42_SEQUENCE=TGGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_42=908,20
PRIMER_RIGHT_42=1585,21
PRIMER_LEFT_42_TM=59.245
PRIMER_RIGHT_42_TM=63.111
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=8.1000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=678
PRIMER_PAIR_43_PENALTY=4.940914
PRIMER_LEFT_43_PENALTY=4.477233
PRIMER_RIGHT_43_PENALTY=0.463680
PRIMER_LEFT_43_SEQUENCE=TCAGGAATTTCTCCTTCAAGTC
PRIMER_RIGHT_43_SEQUENCE=GGTTATGGAGCCTTCCTCGT
PRIMER_LEFT_43=726,22
PRIMER_RIGHT_43=1584,20
PRIMER_LEFT_43_TM=57.523
PRIMER_RIGHT_43_TM=60.464
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=8.1000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=859
PRIMER_PAIR_44_PENALTY=4.965442
PRIMER_LEFT_44_PENALTY=0.754768
PRIMER_RIGHT_44_PENALTY=4.210675
PRIMER_LEFT_44_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_44_SEQUENCE=GATTCATACATTCCATCGACC
PRIMER_LEFT_44=908,20
PRIMER_RIGHT_44=1726,21
PRIMER_LEFT_44_TM=59.245
PRIMER_RIGHT_44_TM=56.789
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=9.6000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=819
PRIMER_PAIR_45_PENALTY=5.104627
PRIMER_LEFT_45_PENALTY=2.697664
PRIMER_RIGHT_45_PENALTY=2.406964
PRIMER_LEFT_45_SEQUENCE=TCGTGAGTGATGATGCTTTC
PRIMER_RIGHT_45_SEQUENCE=GGATTCATACATTCCATCGACC
PRIMER_LEFT_45=907,20
PRIMER_RIGHT_45=1727,22
PRIMER_LEFT_45_TM=57.302
PRIMER_RIGHT_45_TM=60.407
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=9.6000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=821
PRIMER_PAIR_46_PENALTY=5.105846
PRIMER_LEFT_46_PENALTY=0.754768
PRIMER_RIGHT_46_PENALTY=4.351079
PRIMER_LEFT_46_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_46_SEQUENCE=GTTATGGAGCCTTCCTCGTCTG
PRIMER_LEFT_46=908,20
PRIMER_RIGHT_46=1583,22
PRIMER_LEFT_46_TM=59.245
PRIMER_RIGHT_46_TM=62.351
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=54.545
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=676
PRIMER_PAIR_47_PENALTY=5.165799
PRIMER_LEFT_47_PENALTY=3.058797
PRIMER_RIGHT_47_PENALTY=2.107002
PRIMER_LEFT_47_SEQUENCE=TGTATGCCAAAAACGAGAGA
PRIMER_RIGHT_47_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_47=550,20
PRIMER_RIGHT_47=1392,20
PRIMER_LEFT_47_TM=56.941
PRIMER_RIGHT_47_TM=57.893
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=843
PRIMER_PAIR_48_PENALTY=5.200796
PRIMER_LEFT_48_PENALTY=3.093794
PRIMER_RIGHT_48_PENALTY=2.107002
PRIMER_LEFT_48_SEQUENCE=TGTATGCCAAAAACGAGAGAGT
PRIMER_RIGHT_48_SEQUENCE=GAGGCGACATACCTCTTTCA
PRIMER_LEFT_48=550,22
PRIMER_RIGHT_48=1392,20
PRIMER_LEFT_48_TM=58.906
PRIMER_RIGHT_48_TM=57.893
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.1000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=843
PRIMER_PAIR_49_PENALTY=5.250815
PRIMER_LEFT_49_PENALTY=0.754768
PRIMER_RIGHT_49_PENALTY=4.496048
PRIMER_LEFT_49_SEQUENCE=CGTGAGTGATGATGCTTTCC
PRIMER_RIGHT_49_SEQUENCE=CCTAAACACAAGCTACCCAA
PRIMER_LEFT_49=908,20
PRIMER_RIGHT_49=1832,20
PRIMER_LEFT_49_TM=59.245
PRIMER_RIGHT_49_TM=55.504
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=10.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=925
=
SEQUENCE_ID=MAGIC.338_5_15105675_15105713_wu-0_po-0_7175_9810_TRUE
SEQUENCE_TARGET=950,138
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATTTTANTTAAGAAAACAANATATAAACGATGGTGGGAGGTTTAGTTNAAACACCTTACCTANCGATACCTTTACNTTGGAAAGGTTTCTAAGNGGGACNTACCACTAGACTANNNNNNNNATGAGACTATGTACTGTACACAAATATTGAGCTGCAAAANTACAGTAATCTATTTTGANACTGCAATACCAAATTTCCTTTACTTAGGTTTAGTAGTGCATGGCTCGACTTTGAAGAAAATACTTACAAAGGGTANAATATAGCAGTACAAAACAATTGAAATACTTACTCGTCCATGTATGTTGCTTGATATTTTTATTATCTTTCCATTGTCGTTTTGTTATTATTATTGACATTGNAAAAAAACAGAAAANAAACTCATTCTAATCGTTGTCCAATCAGTATGTCATTNAAACAAACCAAAAATGAATNATTTTTTTNTTTACCAATCTTNTTAAGTNAATNGTCACNNAANAANGAGATCANTTATNAAANNNAAAANNNNNNNNNNATATATATAGAAGGANNNNNNATATCACGTNGTTNCGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_10818633_10818779_ler-0_po-0_4574_33961_TRUE
SEQUENCE_TARGET=950,246
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCAATATGAAGTNANCTCACTCAAANACAATTTTCCCAATCGGTTCGTNAGAACGTGATTGTCAGAAATCAAAATCGTTTACTTTTCTCNCNATAGCACTGATATTTTCGATAAAAACAAATCTGTTCGCGTATTNTANTGTNTTATTATGCTCATTTCGACACACAACAAAANCGAACCGNTAAAAAATATATGAACCGGTTTGNTCATCATATTACACTGATATNTATATTCAAAGGAAACTTCCNATGCGGAAGAAGAAAATATCTTCATTAAGTTACAATCANAATATTCGTTACAATTTTCTTGACAAGTTAAGTTATATAATTAAGGGAAATAGNAAANCAAAATTCAAAAGAAAAATCAAGACTATTAANTGAGGCTATATAAGTAACATGTACANAGAAGGAAAAAGGGATTCATAGCCAAAACCATGAAGAGCACGAACGANGAATCTCCTTCCTTGGGATACGANTTCGCCGGAGTTAGCATCAAGGACGGAANATCTAAGCCGCCGGCGCCGGTANTATGTTNCGATGGNTTGTTCGGAGNGAGAGACGTGATTAGAATCGTGCTATTGGTCACGACCGTNACACTTTCTTGTCTATTATTCTACAAATCTGCAAACAATCCACTCAATATGGTGTTNTCTCCATGGAAGACCGATTGTTATGCTTCCAAACTTACAAACGNGTCTTCCTNAAAAACGGTGAGTATTAATAAATATGTGTATGACGTATAATACGAATTTATTGGTATTTTAAAATAAATTTACGAAGGNTTTTCANGGAGTTGTTAATATATCGTTTGGTTTATATNAAAATNTCAGATTCCATTTCAAACTAATCNCTGGAATCTACAATCTTCCGTACCTAAAGAGAGAATTTGTCTATAAATAAAGCTAATCTCTCTAGTCTTGACATTATNACATTAAGACGATNAAAATTACACNCGGTCATGTTGATATGTTNATCTCTCTTAAATGTATCGATGTTTTTAGGAACCAAAGAAGGAGCCGGTTTCAGAACTAGAGAGAGTATTGATGAATGCGGCTATGGAAGATANCACGGTGATCATAACGGCGTTAAACCAAGCATGGGCCGAACCAAACTCTACATTCGATGTGTTTCGAGAGAGTTTTAAAGTCGGNATAGAAACGGAGAGGTTATTGAAGCATGTGATCGCGGTTTGCTTGGATATCAAGGCATATGATCAGTGCCTAAAGGTTCATCCTCATTGCTATTTGATCAACGCCACAGATTCAGACCAACTCTCAGGTCCGAATCGGTTTATGACGCCTGGTTACTTGAAACTTATTTGGCGGCGAATGGATCTTCTTAGGCAAGTTATTGGTTTAGGGTACAACTTCATATTCACGGTACATATACTATCTCAATCTTCTTCTNTTATTTTAATCATAATTAATTAGNGTATGTTTTCTNTATAGGTACCTTTTTTNCTNCAACANTTTGCAATTACTGACTTCTATGCGTTGAATTAGAAAATATNACGCGCCATNCCCCAAGAAATTCAATTACTTTTTAAACTATATAATATCAACAATAACNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATTATAGAAAAATCTTAATATTTGATATAGGTATACCAAAAAACTAAAATTACAGCGTTTTGACTTCTTTGGCATGTATANAAATTTAAGTTTTAAGAATAATATATAAGGATTCGATTGTACATAAAAGNGAAATGTTAAGTTTATGAAATATCTATATGTCAACTATTGAGAACACCGTTTNACTATGCATATGCTTTGTTTTAANTATTGTCCTTTTGTTAGATCTTACTTTTACTTGAGCATTTAGATTTGACTATCGTGTTTCTTTGTTTTTAGCAAACNTNTGACTTCCAATTTTACGCTTAATTTTCCTTGATTCATTTATATTAATTTGTGGCTGGCTATAGATGTTTAATTGTCAACTTTATACC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.322250
PRIMER_LEFT_0_PENALTY=0.159750
PRIMER_RIGHT_0_PENALTY=0.162500
PRIMER_LEFT_0_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_0_SEQUENCE=GTCTGAATCTGTGGCGTTGA
PRIMER_LEFT_0=602,20
PRIMER_RIGHT_0=1442,20
PRIMER_LEFT_0_TM=59.840
PRIMER_RIGHT_0_TM=59.837
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=841
PRIMER_PAIR_1_PENALTY=0.325243
PRIMER_LEFT_1_PENALTY=0.159750
PRIMER_RIGHT_1_PENALTY=0.165494
PRIMER_LEFT_1_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_1_SEQUENCE=TCGGACCTGAGAGTTGGTCT
PRIMER_LEFT_1=602,20
PRIMER_RIGHT_1=1458,20
PRIMER_LEFT_1_TM=59.840
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=857
PRIMER_PAIR_2_PENALTY=0.397365
PRIMER_LEFT_2_PENALTY=0.159750
PRIMER_RIGHT_2_PENALTY=0.237616
PRIMER_LEFT_2_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_2_SEQUENCE=TTCGGACCTGAGAGTTGGTC
PRIMER_LEFT_2=602,20
PRIMER_RIGHT_2=1459,20
PRIMER_LEFT_2_TM=59.840
PRIMER_RIGHT_2_TM=60.238
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=858
PRIMER_PAIR_3_PENALTY=0.400579
PRIMER_LEFT_3_PENALTY=0.238079
PRIMER_RIGHT_3_PENALTY=0.162500
PRIMER_LEFT_3_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_3_SEQUENCE=GTCTGAATCTGTGGCGTTGA
PRIMER_LEFT_3=590,20
PRIMER_RIGHT_3=1442,20
PRIMER_LEFT_3_TM=59.762
PRIMER_RIGHT_3_TM=59.837
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=853
PRIMER_PAIR_4_PENALTY=0.439345
PRIMER_LEFT_4_PENALTY=0.159750
PRIMER_RIGHT_4_PENALTY=0.279595
PRIMER_LEFT_4_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_4_SEQUENCE=GCCGCCAAATAAGTTTCAAG
PRIMER_LEFT_4=602,20
PRIMER_RIGHT_4=1500,20
PRIMER_LEFT_4_TM=59.840
PRIMER_RIGHT_4_TM=59.720
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=899
PRIMER_PAIR_5_PENALTY=0.468650
PRIMER_LEFT_5_PENALTY=0.306150
PRIMER_RIGHT_5_PENALTY=0.162500
PRIMER_LEFT_5_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_5_SEQUENCE=GTCTGAATCTGTGGCGTTGA
PRIMER_LEFT_5=657,20
PRIMER_RIGHT_5=1442,20
PRIMER_LEFT_5_TM=59.694
PRIMER_RIGHT_5_TM=59.837
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=786
PRIMER_PAIR_6_PENALTY=0.471643
PRIMER_LEFT_6_PENALTY=0.306150
PRIMER_RIGHT_6_PENALTY=0.165494
PRIMER_LEFT_6_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_6_SEQUENCE=TCGGACCTGAGAGTTGGTCT
PRIMER_LEFT_6=657,20
PRIMER_RIGHT_6=1458,20
PRIMER_LEFT_6_TM=59.694
PRIMER_RIGHT_6_TM=59.835
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=802
PRIMER_PAIR_7_PENALTY=0.471643
PRIMER_LEFT_7_PENALTY=0.306150
PRIMER_RIGHT_7_PENALTY=0.165494
PRIMER_LEFT_7_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_7_SEQUENCE=TCGGACCTGAGAGTTGGTCT
PRIMER_LEFT_7=655,20
PRIMER_RIGHT_7=1458,20
PRIMER_LEFT_7_TM=59.694
PRIMER_RIGHT_7_TM=59.835
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=804
PRIMER_PAIR_8_PENALTY=0.475695
PRIMER_LEFT_8_PENALTY=0.238079
PRIMER_RIGHT_8_PENALTY=0.237616
PRIMER_LEFT_8_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_8_SEQUENCE=TTCGGACCTGAGAGTTGGTC
PRIMER_LEFT_8=590,20
PRIMER_RIGHT_8=1459,20
PRIMER_LEFT_8_TM=59.762
PRIMER_RIGHT_8_TM=60.238
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=870
PRIMER_PAIR_9_PENALTY=0.504963
PRIMER_LEFT_9_PENALTY=0.159750
PRIMER_RIGHT_9_PENALTY=0.345214
PRIMER_LEFT_9_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_9_SEQUENCE=GATTCGGACCTGAGAGTTGG
PRIMER_LEFT_9=602,20
PRIMER_RIGHT_9=1461,20
PRIMER_LEFT_9_TM=59.840
PRIMER_RIGHT_9_TM=59.655
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=860
PRIMER_PAIR_10_PENALTY=0.517674
PRIMER_LEFT_10_PENALTY=0.238079
PRIMER_RIGHT_10_PENALTY=0.279595
PRIMER_LEFT_10_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_10_SEQUENCE=GCCGCCAAATAAGTTTCAAG
PRIMER_LEFT_10=590,20
PRIMER_RIGHT_10=1500,20
PRIMER_LEFT_10_TM=59.762
PRIMER_RIGHT_10_TM=59.720
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=911
PRIMER_PAIR_11_PENALTY=0.543765
PRIMER_LEFT_11_PENALTY=0.306150
PRIMER_RIGHT_11_PENALTY=0.237616
PRIMER_LEFT_11_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_11_SEQUENCE=TTCGGACCTGAGAGTTGGTC
PRIMER_LEFT_11=657,20
PRIMER_RIGHT_11=1459,20
PRIMER_LEFT_11_TM=59.694
PRIMER_RIGHT_11_TM=60.238
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.6000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=803
PRIMER_PAIR_12_PENALTY=0.543765
PRIMER_LEFT_12_PENALTY=0.306150
PRIMER_RIGHT_12_PENALTY=0.237616
PRIMER_LEFT_12_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_12_SEQUENCE=TTCGGACCTGAGAGTTGGTC
PRIMER_LEFT_12=655,20
PRIMER_RIGHT_12=1459,20
PRIMER_LEFT_12_TM=59.694
PRIMER_RIGHT_12_TM=60.238
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=805
PRIMER_PAIR_13_PENALTY=0.583292
PRIMER_LEFT_13_PENALTY=0.238079
PRIMER_RIGHT_13_PENALTY=0.345214
PRIMER_LEFT_13_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_13_SEQUENCE=GATTCGGACCTGAGAGTTGG
PRIMER_LEFT_13=590,20
PRIMER_RIGHT_13=1461,20
PRIMER_LEFT_13_TM=59.762
PRIMER_RIGHT_13_TM=59.655
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=872
PRIMER_PAIR_14_PENALTY=0.585745
PRIMER_LEFT_14_PENALTY=0.306150
PRIMER_RIGHT_14_PENALTY=0.279595
PRIMER_LEFT_14_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_14_SEQUENCE=GCCGCCAAATAAGTTTCAAG
PRIMER_LEFT_14=657,20
PRIMER_RIGHT_14=1500,20
PRIMER_LEFT_14_TM=59.694
PRIMER_RIGHT_14_TM=59.720
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=844
PRIMER_PAIR_15_PENALTY=0.585745
PRIMER_LEFT_15_PENALTY=0.306150
PRIMER_RIGHT_15_PENALTY=0.279595
PRIMER_LEFT_15_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_15_SEQUENCE=GCCGCCAAATAAGTTTCAAG
PRIMER_LEFT_15=655,20
PRIMER_RIGHT_15=1500,20
PRIMER_LEFT_15_TM=59.694
PRIMER_RIGHT_15_TM=59.720
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=846
PRIMER_PAIR_16_PENALTY=0.651363
PRIMER_LEFT_16_PENALTY=0.306150
PRIMER_RIGHT_16_PENALTY=0.345214
PRIMER_LEFT_16_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_16_SEQUENCE=GATTCGGACCTGAGAGTTGG
PRIMER_LEFT_16=657,20
PRIMER_RIGHT_16=1461,20
PRIMER_LEFT_16_TM=59.694
PRIMER_RIGHT_16_TM=59.655
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=805
PRIMER_PAIR_17_PENALTY=0.651363
PRIMER_LEFT_17_PENALTY=0.306150
PRIMER_RIGHT_17_PENALTY=0.345214
PRIMER_LEFT_17_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_17_SEQUENCE=GATTCGGACCTGAGAGTTGG
PRIMER_LEFT_17=655,20
PRIMER_RIGHT_17=1461,20
PRIMER_LEFT_17_TM=59.694
PRIMER_RIGHT_17_TM=59.655
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=807
PRIMER_PAIR_18_PENALTY=0.733398
PRIMER_LEFT_18_PENALTY=0.159750
PRIMER_RIGHT_18_PENALTY=0.573649
PRIMER_LEFT_18_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_18_SEQUENCE=CTTCCATAGCCGCATTCATC
PRIMER_LEFT_18=602,20
PRIMER_RIGHT_18=1236,20
PRIMER_LEFT_18_TM=59.840
PRIMER_RIGHT_18_TM=60.574
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=635
PRIMER_PAIR_19_PENALTY=0.733398
PRIMER_LEFT_19_PENALTY=0.159750
PRIMER_RIGHT_19_PENALTY=0.573649
PRIMER_LEFT_19_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_19_SEQUENCE=ATCTTCCATAGCCGCATTCA
PRIMER_LEFT_19=602,20
PRIMER_RIGHT_19=1238,20
PRIMER_LEFT_19_TM=59.840
PRIMER_RIGHT_19_TM=60.574
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=637
PRIMER_PAIR_20_PENALTY=0.811727
PRIMER_LEFT_20_PENALTY=0.238079
PRIMER_RIGHT_20_PENALTY=0.573649
PRIMER_LEFT_20_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_20_SEQUENCE=CTTCCATAGCCGCATTCATC
PRIMER_LEFT_20=590,20
PRIMER_RIGHT_20=1236,20
PRIMER_LEFT_20_TM=59.762
PRIMER_RIGHT_20_TM=60.574
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=647
PRIMER_PAIR_21_PENALTY=0.811727
PRIMER_LEFT_21_PENALTY=0.238079
PRIMER_RIGHT_21_PENALTY=0.573649
PRIMER_LEFT_21_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_21_SEQUENCE=ATCTTCCATAGCCGCATTCA
PRIMER_LEFT_21=590,20
PRIMER_RIGHT_21=1238,20
PRIMER_LEFT_21_TM=59.762
PRIMER_RIGHT_21_TM=60.574
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=649
PRIMER_PAIR_22_PENALTY=0.842435
PRIMER_LEFT_22_PENALTY=0.159750
PRIMER_RIGHT_22_PENALTY=0.682685
PRIMER_LEFT_22_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_22_SEQUENCE=TTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_22=602,20
PRIMER_RIGHT_22=1487,20
PRIMER_LEFT_22_TM=59.840
PRIMER_RIGHT_22_TM=59.317
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.4000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=886
PRIMER_PAIR_23_PENALTY=0.879798
PRIMER_LEFT_23_PENALTY=0.306150
PRIMER_RIGHT_23_PENALTY=0.573649
PRIMER_LEFT_23_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_23_SEQUENCE=CTTCCATAGCCGCATTCATC
PRIMER_LEFT_23=657,20
PRIMER_RIGHT_23=1236,20
PRIMER_LEFT_23_TM=59.694
PRIMER_RIGHT_23_TM=60.574
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.6000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=580
PRIMER_PAIR_24_PENALTY=0.879798
PRIMER_LEFT_24_PENALTY=0.306150
PRIMER_RIGHT_24_PENALTY=0.573649
PRIMER_LEFT_24_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_24_SEQUENCE=ATCTTCCATAGCCGCATTCA
PRIMER_LEFT_24=657,20
PRIMER_RIGHT_24=1238,20
PRIMER_LEFT_24_TM=59.694
PRIMER_RIGHT_24_TM=60.574
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.6000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=582
PRIMER_PAIR_25_PENALTY=0.879798
PRIMER_LEFT_25_PENALTY=0.306150
PRIMER_RIGHT_25_PENALTY=0.573649
PRIMER_LEFT_25_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_25_SEQUENCE=CTTCCATAGCCGCATTCATC
PRIMER_LEFT_25=655,20
PRIMER_RIGHT_25=1236,20
PRIMER_LEFT_25_TM=59.694
PRIMER_RIGHT_25_TM=60.574
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=582
PRIMER_PAIR_26_PENALTY=0.879798
PRIMER_LEFT_26_PENALTY=0.306150
PRIMER_RIGHT_26_PENALTY=0.573649
PRIMER_LEFT_26_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_26_SEQUENCE=ATCTTCCATAGCCGCATTCA
PRIMER_LEFT_26=655,20
PRIMER_RIGHT_26=1238,20
PRIMER_LEFT_26_TM=59.694
PRIMER_RIGHT_26_TM=60.574
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=584
PRIMER_PAIR_27_PENALTY=0.920764
PRIMER_LEFT_27_PENALTY=0.238079
PRIMER_RIGHT_27_PENALTY=0.682685
PRIMER_LEFT_27_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_27_SEQUENCE=TTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_27=590,20
PRIMER_RIGHT_27=1487,20
PRIMER_LEFT_27_TM=59.762
PRIMER_RIGHT_27_TM=59.317
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=8.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=898
PRIMER_PAIR_28_PENALTY=0.938218
PRIMER_LEFT_28_PENALTY=0.775718
PRIMER_RIGHT_28_PENALTY=0.162500
PRIMER_LEFT_28_SEQUENCE=AGCCAAAACCATGAAGAGCA
PRIMER_RIGHT_28_SEQUENCE=GTCTGAATCTGTGGCGTTGA
PRIMER_LEFT_28=599,20
PRIMER_RIGHT_28=1442,20
PRIMER_LEFT_28_TM=60.776
PRIMER_RIGHT_28_TM=59.837
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=844
PRIMER_PAIR_29_PENALTY=0.950337
PRIMER_LEFT_29_PENALTY=0.159750
PRIMER_RIGHT_29_PENALTY=0.790588
PRIMER_LEFT_29_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_29_SEQUENCE=CGATTCGGACCTGAGAGTTG
PRIMER_LEFT_29=602,20
PRIMER_RIGHT_29=1462,20
PRIMER_LEFT_29_TM=59.840
PRIMER_RIGHT_29_TM=60.791
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.4000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=861
PRIMER_PAIR_30_PENALTY=0.988835
PRIMER_LEFT_30_PENALTY=0.306150
PRIMER_RIGHT_30_PENALTY=0.682685
PRIMER_LEFT_30_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_30_SEQUENCE=TTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_30=657,20
PRIMER_RIGHT_30=1487,20
PRIMER_LEFT_30_TM=59.694
PRIMER_RIGHT_30_TM=59.317
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=831
PRIMER_PAIR_31_PENALTY=0.988835
PRIMER_LEFT_31_PENALTY=0.306150
PRIMER_RIGHT_31_PENALTY=0.682685
PRIMER_LEFT_31_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_31_SEQUENCE=TTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_31=655,20
PRIMER_RIGHT_31=1487,20
PRIMER_LEFT_31_TM=59.694
PRIMER_RIGHT_31_TM=59.317
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=833
PRIMER_PAIR_32_PENALTY=1.009982
PRIMER_LEFT_32_PENALTY=0.159750
PRIMER_RIGHT_32_PENALTY=0.850232
PRIMER_LEFT_32_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_32_SEQUENCE=CGGACCTGAGAGTTGGTCTG
PRIMER_LEFT_32=602,20
PRIMER_RIGHT_32=1457,20
PRIMER_LEFT_32_TM=59.840
PRIMER_RIGHT_32_TM=60.850
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=60.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=856
PRIMER_PAIR_33_PENALTY=1.013334
PRIMER_LEFT_33_PENALTY=0.775718
PRIMER_RIGHT_33_PENALTY=0.237616
PRIMER_LEFT_33_SEQUENCE=AGCCAAAACCATGAAGAGCA
PRIMER_RIGHT_33_SEQUENCE=TTCGGACCTGAGAGTTGGTC
PRIMER_LEFT_33=599,20
PRIMER_RIGHT_33=1459,20
PRIMER_LEFT_33_TM=60.776
PRIMER_RIGHT_33_TM=60.238
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=861
PRIMER_PAIR_34_PENALTY=1.028666
PRIMER_LEFT_34_PENALTY=0.238079
PRIMER_RIGHT_34_PENALTY=0.790588
PRIMER_LEFT_34_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_34_SEQUENCE=CGATTCGGACCTGAGAGTTG
PRIMER_LEFT_34=590,20
PRIMER_RIGHT_34=1462,20
PRIMER_LEFT_34_TM=59.762
PRIMER_RIGHT_34_TM=60.791
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=873
PRIMER_PAIR_35_PENALTY=1.055313
PRIMER_LEFT_35_PENALTY=0.775718
PRIMER_RIGHT_35_PENALTY=0.279595
PRIMER_LEFT_35_SEQUENCE=AGCCAAAACCATGAAGAGCA
PRIMER_RIGHT_35_SEQUENCE=GCCGCCAAATAAGTTTCAAG
PRIMER_LEFT_35=599,20
PRIMER_RIGHT_35=1500,20
PRIMER_LEFT_35_TM=60.776
PRIMER_RIGHT_35_TM=59.720
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=902
PRIMER_PAIR_36_PENALTY=1.088311
PRIMER_LEFT_36_PENALTY=0.238079
PRIMER_RIGHT_36_PENALTY=0.850232
PRIMER_LEFT_36_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_36_SEQUENCE=CGGACCTGAGAGTTGGTCTG
PRIMER_LEFT_36=590,20
PRIMER_RIGHT_36=1457,20
PRIMER_LEFT_36_TM=59.762
PRIMER_RIGHT_36_TM=60.850
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=60.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=868
PRIMER_PAIR_37_PENALTY=1.096737
PRIMER_LEFT_37_PENALTY=0.306150
PRIMER_RIGHT_37_PENALTY=0.790588
PRIMER_LEFT_37_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_37_SEQUENCE=CGATTCGGACCTGAGAGTTG
PRIMER_LEFT_37=657,20
PRIMER_RIGHT_37=1462,20
PRIMER_LEFT_37_TM=59.694
PRIMER_RIGHT_37_TM=60.791
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=806
PRIMER_PAIR_38_PENALTY=1.096737
PRIMER_LEFT_38_PENALTY=0.306150
PRIMER_RIGHT_38_PENALTY=0.790588
PRIMER_LEFT_38_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_38_SEQUENCE=CGATTCGGACCTGAGAGTTG
PRIMER_LEFT_38=655,20
PRIMER_RIGHT_38=1462,20
PRIMER_LEFT_38_TM=59.694
PRIMER_RIGHT_38_TM=60.791
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=808
PRIMER_PAIR_39_PENALTY=1.120932
PRIMER_LEFT_39_PENALTY=0.775718
PRIMER_RIGHT_39_PENALTY=0.345214
PRIMER_LEFT_39_SEQUENCE=AGCCAAAACCATGAAGAGCA
PRIMER_RIGHT_39_SEQUENCE=GATTCGGACCTGAGAGTTGG
PRIMER_LEFT_39=599,20
PRIMER_RIGHT_39=1461,20
PRIMER_LEFT_39_TM=60.776
PRIMER_RIGHT_39_TM=59.655
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=863
PRIMER_PAIR_40_PENALTY=1.156382
PRIMER_LEFT_40_PENALTY=0.306150
PRIMER_RIGHT_40_PENALTY=0.850232
PRIMER_LEFT_40_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_40_SEQUENCE=CGGACCTGAGAGTTGGTCTG
PRIMER_LEFT_40=657,20
PRIMER_RIGHT_40=1457,20
PRIMER_LEFT_40_TM=59.694
PRIMER_RIGHT_40_TM=60.850
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=60.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=801
PRIMER_PAIR_41_PENALTY=1.156382
PRIMER_LEFT_41_PENALTY=0.306150
PRIMER_RIGHT_41_PENALTY=0.850232
PRIMER_LEFT_41_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_41_SEQUENCE=CGGACCTGAGAGTTGGTCTG
PRIMER_LEFT_41=655,20
PRIMER_RIGHT_41=1457,20
PRIMER_LEFT_41_TM=59.694
PRIMER_RIGHT_41_TM=60.850
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=60.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=803
PRIMER_PAIR_42_PENALTY=1.320840
PRIMER_LEFT_42_PENALTY=0.159750
PRIMER_RIGHT_42_PENALTY=1.161090
PRIMER_LEFT_42_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_42_SEQUENCE=GTTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_42=602,20
PRIMER_RIGHT_42=1488,21
PRIMER_LEFT_42_TM=59.840
PRIMER_RIGHT_42_TM=60.161
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=887
PRIMER_PAIR_43_PENALTY=1.349367
PRIMER_LEFT_43_PENALTY=0.775718
PRIMER_RIGHT_43_PENALTY=0.573649
PRIMER_LEFT_43_SEQUENCE=AGCCAAAACCATGAAGAGCA
PRIMER_RIGHT_43_SEQUENCE=CTTCCATAGCCGCATTCATC
PRIMER_LEFT_43=599,20
PRIMER_RIGHT_43=1236,20
PRIMER_LEFT_43_TM=60.776
PRIMER_RIGHT_43_TM=60.574
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=638
PRIMER_PAIR_44_PENALTY=1.349367
PRIMER_LEFT_44_PENALTY=0.775718
PRIMER_RIGHT_44_PENALTY=0.573649
PRIMER_LEFT_44_SEQUENCE=AGCCAAAACCATGAAGAGCA
PRIMER_RIGHT_44_SEQUENCE=ATCTTCCATAGCCGCATTCA
PRIMER_LEFT_44=599,20
PRIMER_RIGHT_44=1238,20
PRIMER_LEFT_44_TM=60.776
PRIMER_RIGHT_44_TM=60.574
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=640
PRIMER_PAIR_45_PENALTY=1.399169
PRIMER_LEFT_45_PENALTY=0.238079
PRIMER_RIGHT_45_PENALTY=1.161090
PRIMER_LEFT_45_SEQUENCE=GGGATTCATAGCCAAAACCA
PRIMER_RIGHT_45_SEQUENCE=GTTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_45=590,20
PRIMER_RIGHT_45=1488,21
PRIMER_LEFT_45_TM=59.762
PRIMER_RIGHT_45_TM=60.161
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=899
PRIMER_PAIR_46_PENALTY=1.419999
PRIMER_LEFT_46_PENALTY=0.159750
PRIMER_RIGHT_46_PENALTY=1.260249
PRIMER_LEFT_46_SEQUENCE=CAAAACCATGAAGAGCACGA
PRIMER_RIGHT_46_SEQUENCE=CCTAAGAAGATCCATTCGCC
PRIMER_LEFT_46=602,20
PRIMER_RIGHT_46=1517,20
PRIMER_LEFT_46_TM=59.840
PRIMER_RIGHT_46_TM=58.740
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.4000
PRIMER_RIGHT_46_END_STABILITY=11.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=916
PRIMER_PAIR_47_PENALTY=1.458403
PRIMER_LEFT_47_PENALTY=0.775718
PRIMER_RIGHT_47_PENALTY=0.682685
PRIMER_LEFT_47_SEQUENCE=AGCCAAAACCATGAAGAGCA
PRIMER_RIGHT_47_SEQUENCE=TTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_47=599,20
PRIMER_RIGHT_47=1487,20
PRIMER_LEFT_47_TM=60.776
PRIMER_RIGHT_47_TM=59.317
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=889
PRIMER_PAIR_48_PENALTY=1.467240
PRIMER_LEFT_48_PENALTY=0.306150
PRIMER_RIGHT_48_PENALTY=1.161090
PRIMER_LEFT_48_SEQUENCE=GGAGTTAGCATCAAGGACGG
PRIMER_RIGHT_48_SEQUENCE=GTTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_48=657,20
PRIMER_RIGHT_48=1488,21
PRIMER_LEFT_48_TM=59.694
PRIMER_RIGHT_48_TM=60.161
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=832
PRIMER_PAIR_49_PENALTY=1.467240
PRIMER_LEFT_49_PENALTY=0.306150
PRIMER_RIGHT_49_PENALTY=1.161090
PRIMER_LEFT_49_SEQUENCE=CCGGAGTTAGCATCAAGGAC
PRIMER_RIGHT_49_SEQUENCE=GTTTCAAGTAACCAGGCGTCA
PRIMER_LEFT_49=655,20
PRIMER_RIGHT_49=1488,21
PRIMER_LEFT_49_TM=59.694
PRIMER_RIGHT_49_TM=60.161
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=834
=
SEQUENCE_ID=MAGIC.105_2_5476640_5476707_tsu-0_ler-0_3993_1077223_TRUE
SEQUENCE_TARGET=950,167
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4614662_4615397_wu-0_can-0_42036_19430_TRUE
SEQUENCE_TARGET=950,835
SEQUENCE_TEMPLATE=GAATGANCGACANAGGCAAAGACACTCNNCTTCANTATTACANGACATNANCAACGTNTATCATCAATTCNCACACCTCTTNCACATCCACACACTTTCTCTTNTTCTGCAATTAANANTTCAACAGGTTAGTTTGACTTCTTNNAGAGCNATTCAATGGANNTGNANCATTTTATNTATAGATNATAANTNACAAAATAAATNTCATATTCTATGCAANGAAAANTTTATNGACATCCTAATTCATGTATTACCAATCNGCCACNATCAATTATTAATTNTCCATCTAGCAACTTTCATATCAGAANCNTACAAGAAGACTTCANAAATGCTTCTCTAACTCCTACTGATAAAGGTAATATACTAANATATTCATCATTGAACTATATGTTTATGGATNATAAACAGTCNCANNNANTTTTTNTTATTTTACAATTNATAGGCAAANCTCCAATGTGTACGCAACCAGCCTACAATNATAATAGCANTNATGACGAAAGCATGNAACATCTTCAACGANAATATATAACTGACAANGAAACTGANAGTGAACAAAATTACTATCNTAGTGCTTAANAGGACGANTGTTCTNNAGAGAATTNTNTNNNNNNNNNNNNNNTTACTTAATAACATTNNTANAAAATGTGNNGANGAATNTTNGTTTNGCTGACGNAAAATGTCGAATTAATATTNTCACNTGAAGATACTCTTANTNAATGTGGAGAAATANAACAAACAGTNNCCTCTGTTNCNCANCCGAAAAAGAGAGGCACACCATTTGGAAAACAACAANGTNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNNNNNNAAAATCAGCTTATAGCTTTCTTTANNNNNNNNNTAGTTGGTTTNNTTNTGNTTAACTTCTACATNGGNTNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAANNANCNTGTTTTAGNGCNAAAAAAGAGANNNCGTCCTANNNNNNNNCNTNCAACTAAAAAAGGAGCAGCTTTAGCCAANANAATTGGTAAGTGTCATNNNTTTTATTNGAATATTGTGTTCTTCAGTTTNNNNNNNNNATTAAATCTGAATACTTTGTTATTCTCTATTGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_13459154_13459163_oy-0_zu-0_19199_1044022_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=TGGGAAATTGGACTTTACGTTNGCGTCGCCTNGAAATTAAGACTCACGCGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNGNANCCACACACTTCNGCAAACTCCNACANTCGTAGCCAAATTGTGTTTATACCAATCAGGNCATTAATATTTTTATTTCTTACCCCTNNNAGCTATNCCTAACTATTATTATGACACTACTACACCTTTCATAGAAGATATCCTGGATTGTGGGTTGTAATCTCTAACTATTATGGNAAAANATATACCGCTGTAACCNATAAAAATACAATAAANAAAAAGTGGTCAATANCAACATTTTAAAATNCCAACATGCAAACGGAATNNTATAATGATCAAAATGTGATAGGTATGAAAAAGAAAAAAANAGTGAAGGGATGAAAATTCNTTNATAANAAGGAAANATAANTATGANTGTTTATTANTATTTTTGTGTCAGTTTTCTTCTTTAAATACATGTTNAATATTAAAAANTTAAGAATATTNTCATGATATTATAANTATGCGTATATTAACTCANAAACAATAAAANCAAAAAANCATTATTNTCNTCGAAAAANAGTNGTTGACCNGATTCTNAGAGTAGAAAGTCATTTGTTAAATTGCTACATAGAATTAANGAANNTTTTNTAATAAGAATTAAAANAATTAATGAGTTGCTTTTCTTAAACTCTTGNTNGAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4782538_4782580_wu-0_edi-0_37915_9143_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_11579021_11579038_no-0_po-0_61950_71994_TRUE
SEQUENCE_TARGET=950,117
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_5882346_5882563_kn-0_ler-0_116104_121639_TRUE
SEQUENCE_TARGET=950,317
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTANNNCANNNNNNNNNAGNATNCCGGNCNCTANTTGGTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTTATGTTTGATTTTGGTTATAACTATCTAGTCATNAAAATAANAAAANNNTATATGTNAAANNATTGNNNGCTAATTTCAAANTTGCANANNNNNAAANNAANANANGAAANTTGTTCGTAATANTGCTTTCTCTATTACAACTAANTAAATTATNTCGGATCNCAAATNCTAATCATNAAGAATATANACTNGTTACAAATAAANTGAAAACAACTNCGTACACTAAATCCTAANANATTAAGGNCGNGGAGTAACNNCTNNNTNTATATANACACANAGTTTAACTTATTCTACTANANNAAAAAAAAACNNAAGAGANANTTCATAAACAATGACGNTCNAANAAGAGGTGTCTANAGAANATATNNNGTGTNTGGAAACANTACTGATCGATCNATCNTGAGCTGNNGGACNATTTTCTTNGNCGGATGAGTGTTGNNNNGATCGATCNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_16734511_16735251_tsu-0_kn-0_37576_145549_TRUE
SEQUENCE_TARGET=950,840
SEQUENCE_TEMPLATE=TATATTAAAAAAAATCATCATGCAGAATGACTTCTAGTATACACGTACACATGTACATCATGGATGATGTTTTTATTGAAAGTTGATAAACTACACTGACTATGTTTTCAGTTCAATTAGCTAGCCTTGCTTCGAATATATACTTTGGTGTAGTATCATCATTAATTTGTATATAGTTTTATAATTTTTGATTATGGAGCCTNTGCGTGTCTTGGTTTCCTTCGATATATTAATGGTAGTGCCAAAATAATGTTTAATGTAATTTTGCCAAGCCAGAAAATTACAGATCCACAACTAGCTAGTGTGGGGNTGTTAATAAGACAACAAATTTTTTAACCGTGTTTACTGTCACTATAGATAGGCATGTACGCTAGTTAAGTGAAGTCACACTGATTACGAAAGGATGATTAGATTATACTATATGATATATCATGCATTAATGTAATTAAAAAACTTGCGATTTCATTCACGACGCTTTGTAAGAGAGGATTTCTTTCAATATATTAATAGAAAGCTTTAAAAATGGAAGTAATGAAATTGCGTTAATCTCGTNTGTTCTGTGGACGTCAAAGATTTTTTAAAATAAAGATTACACTCCGTGAGTGTCGCTAATCTCGTGTACTATTACTTTTTACAGTGTCTCTTTTCTCACATTTTATATGGTAATAACAAAAAAAAGGCAGNAAGATACAAGATACAAGATTATAGTTGACGTCAATAGTAAAACAGAAAACATAGGCAATAGTTAGAAAAAAAATNAAAAAAATTCAAGGAGGTTTATNAGTTTGTNAGTTTTAACAGATATGTGTCAAGTTGGAAAAACAAAAGTTNAAATATGAGGGTGAAAATTGTTATTTCGAACGCATTTCTAAATAATAGTTAAAATTTACATATATCATTTTAATTCCNTTTTTTTNGCTTTGTAATATTCNTTTTTTTAANAAAAATCGAAAACTTTCAAGGATTTTCNAAATGACCAAATCAATGAAATAATACGTAGNCAATAAATTCCTCTAAACATGAAAAGTAAAAGAGGTTNCTTATTGGGATGGCCTCTTTGATATATGTAAACACAAAAAATGTTGAATCAGAGCATGGACGGCATAAACACACATAACCTACACATCTGATCACTTGACCTTGATCTGAGGTCCTATTTTAAACATGTTAAAAACAAGGTTGGATTTTTCGAAAAACTTTGCAAAGAGTTACGCACACAAAAGTTGAAAGTTCTGGTGTGACATCTATTCTCGGTGGGAAATNTTTCCAAAAACACCAGTACAATATTTTGTCTGAGCCATTTTGTGCAGGGACCATGATCATACTCGATTATTAATTATGTCTCTTGCTTTCATTGAATATGATATTTTTTCTCATAAATTTGTTGTTGTCAACNAAACTGTTCATGTAGNGTGGTTTGCCCTTTACAAACACATTTAAGTCTTCACATCTTCGGACCTATTCCTGAAATTGTTTTCCATCAAATATTATTTTCTTATATGTCTTTAATTTCAACACCATCACANCTTCCGTTAGTCAAACCAGATAATTAAAAGGACATAAGTTATTTAAGAAAGCTTTAAAANTTTTCCGTTGCTTATATGTTATAACTATTTTTGATAAAGACAAAAAATATGTTATAACTTATAAACACTTAAGTACTCTAATTAATTACATAATTTAATACAGTTTTGTTTATATTTTATTTNATCCTATAAATTCTAAACTNTTTTCCCTACCNAAACCATCCAAATATCAAAGCAAATAGAAGTCAAAGANAGATGGGAAACAATTCAAGAGCTATTTTCTCTTTATTNTTGGTTGTAGCNTTATGTGCTGCAATTTTAATCACNCCCGGAATAGCTCATGATGAACAACCACCGCCCCGCTCACAATTTCCACCGGATTTTCCTATTGATGTGGAAAAATGTTGGNCATCACTTTTCAATACTCAGGGATGCGTATTCGAACTTTTAAAGTCAGTTTTTTCTGGTCAGTTTGGAAATGTTGGAGTTGCATGTTGCAAAGCATTGTCGACCATAGATGCTAACTGTTGGCCTCATATGTTCCCGCTTAACCCGTTCTTTCCACCTCTGCTTAAGGACAACTGTGCGCACATCGTCCCCAATTCTCCCTCAAAGTGAAAGCGGGAATCAAGTGACTTGATGCTTTATAATATAGTCGAGTTAGTTTNGTAATCTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCTCCTATATATGTTCCAAGTGCCTTATATATCCTTTAATTTACTCTATTCTGTCTTTGTTTACAAATATATATGGAAATAAAAGATATAATAACTTAGTTAGCAACATTCAAGAGAGCATACAGTAAATTAGCTGAAGTATGTAGATTAGTTGGTGGAAATGGTGAAATAAATGAAGATCTTTTGAAGGTCTCTGTCATACACAATCAAGACGATGTTTTATGATCGCAGTTGATTGGAAAAGGACTTGTAACAACCAAACCTATATGTATATCCCTGGACATTTCATTTGTACACTTACCAAAACTTGACCAACCCACTACTTGAATNCGAATATATCCAGTTGGTAAACCGAGTCCGANAACCGGACCAGAATCAAAAACCAAAATCTTAAACCGAATATNTNAAATTTNNANAAAACAAAATTATTTTCAAAGTGAAACAACGACTTTAATATTAGACTTGAAGGCACGACATTAACTCGAG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_16697680_16697796_ler-0_oy-0_18517_196086_TRUE
SEQUENCE_TARGET=950,216
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14038972_14039002_ler-0_zu-0_1195_233349_TRUE
SEQUENCE_TARGET=950,130
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCTNGATCATGTNNTTAAGCTCNNTGTTCGCCTNAGCAANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACCCGANATGAATGATGNGCNTGGAGTAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGATNGTGTNTTTCCNNGTTTTGAAGCNNNGNAANACGTTGCCCTCTTTCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCGAGNGAAATNAANCCNTNGTTNGAACCTNTAAGCNATTTNGTNTGAGGAACGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTNGATCAGNCTTCTCNCCACTAGGTNGNNGAGAAGGNNNNNNNNNNNNNNNNGAGGAGGAGNACCCATCGATCNAGATGGAGGAGATGATCGNNGAGAGCGATCAGTNCGAGCCNTTGACTTTTCCCTCCAAGGAGAAGAGCTGTTNCGAGCTGGTCTCTCNCTCNNTNTNNNNATNAAGTCAGCGATTGGCTTGCTGCTCAGTCCATCACGGGCTNAAGTCCCGTGTGGTNTGGGAGGANGNGGTGGAAGATGATGGAGTTGTCCAGGAGGACTCATNTNGAGNNNNNNNNNNNNNNNNNNNNNNNNGTCGAGGGTAAACAAATTCCAAAGGGTTTCACCTTCGATGACCACTTTGAAGAANNNNNNNNATTCTTCAGTCGGAAGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAACAGAAGANANATATGNTTNTGCCCTAATTTCTACCAGGTTTNNTGGNCCANTTTGATGGAAGCACTTCGNAAAGGCTNNCAATNGAAGCAANGGNNNNNTTNATTAAGAACTANGGATGGTGNNANNNNNNNTNTTTGTTCGGNAGATNGGANATGAGGTTNNTGTTNGCATTNNNGTANGCNNAGAANTAACCNANTTTCTTCGANNAACTTCTTACNCTNAGAAGCTCNTTCAATTNGGNAACTNCNATTATGTACCCAGCNNCCNCNGCGACGGTNATNANCCCTAGAATAGNTNGGAGAAGGTTGGNGGTTAANTGNGGNAGANCNATTTCAAGAGCNGCCAAGNACNGAACGAGNGCCTNGGGANNAGNANAGGNNAGTCNGNANACCGCGAGGTGAATGAGAAGTCNGGATTAAAGGTGGTTNGATTAAAGGGCCNTTGGGCTGGAANGNGNNNNNCCNGGAAGCNCGAAGTCAATTCAAATTCGAACGTNTGAACANTNAATTCGAAGATCGAAACGGNTNCATTTAAGANNNAATAATTNTGTGTAAATNAGANTTNATTGTAATTCTTAGGTATAANTAATAGGNCCAANNNNNNNAAGAGACACACNCATTTGTTTGCAAACTTANTATACANATTTTCTTACAAAACTCATAGNTTTCTNNATAATCAATAGTTNTTTACACTTATTTTTNTCCTCAANAATAATCCNTCTCANTNNNNAAATAAANNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4503196_4503281_can-0_zu-0_17681_20485_TRUE
SEQUENCE_TARGET=950,185
SEQUENCE_TEMPLATE=ATGGGCAAACAATGAATGTACCACACATAAACGTAGCATATGAAGAGCTTCTTTTGAGGATGGACTATCTTGAGTCAATATNCTCAACGATTCTACTTACCNTGCTGTGGATGTCAGATTCACACANTCAAGTATTTNTTTATTTAGTGAGAAGGATTATTCTTNAGGACAAATGTANTNGAAAATAGATTGATGATTATAGAGAAAACTATGAGNTTTNTAANAAAATGTGTATANNANNTTTGCACAAAAATGCNNGTNNNNCTCTACATGGTCTTTGACCCACAATTTATACTCAATGAATACATTTAATTACCATTTATACACAATTATTCTATATTAAATGTGTCTGTTTCAGTCTTCAAATTAACTATTCGAACGTTCGAATTTGAATNGNTTCCAAAATTTCTGGGCTTCGTCCTCTGGCCCANNNGACTCTCCAATCCGGGCAGTTCTGACATCGATGTCCAGTCCACCTNGCNNNNCNNTCACCTCACNTNTACTTTTACTCAGAAAAGCCCATGGGCCTTTCCTCTTTTAGATCNNTNCNNCTTACGNGGCGATCTTTGACCGNTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNATNCCTNAATGNNCAAAGATNNNNCNNAATGACTCTCTGANTTCCCGCCAANNNNNNNNNNNNNNNNNNNNNNNGGAATCACCCATAATTACTCTCGTGACATCTGTCANAGTTATGACCNCATANCAATTATNTACTATTNAANCGGNAAAAACTNTTCCACNTCCTCCANGACTNCCAAGGCNGTTNCGTCATNATCNCTAGCTTGCGTAAGGTAANTTCNCTTTNNNNNNNNNNNNNNNNNNNNCANNNTTNTTTTANAACAAAGCNCCTNTCTTCTTCCTNNNNNNNNNNNNNNNNNNNNGAACCCTCAGACNANNCATTTTTANGATNAATTCNNAAANTGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAANGANNTTTGCACNCGNNNGAATGANATNGCCGNNCCNCANTANCANTCCACNTGNACNCTNCAANCCCTNAATNGATTGNGAGAGCTNTGTCGNNNNNNNNNNNNNANTATGAGGGANTCNCANATGCCNNANCCNANAGANTCGCCGNAAGACCANNNTNCNGGGTTCTTCTNCNTGTNCGANATNTANTTCAAGGGTTGCGGTNTGANNTTTNCNCTTCCNGNAGCCCTNGTTCNGTACTTANCNGCCCTNNAAATTGCCNTGCCCNNNNTNACCCCTNACNTNCTNCGNNCTATNTTAGNGATTATTACNATCGCNNCNNANGCCGGATNTGTNATCNGNGTTCCNNAACTGAATGAACTTCTCAGCNTGAGGAGTTCTTCNAAGAAAGNNGNATACTTCTNGGCTTATNCGAANGCCNACANGAANCTCATCTNNNATCTTNCNAACAANGACGANAACTNGCANCANCCNTGGTTNNTAANNNNNNNNNNNNNNNNNNNNNTNGNAAATCTTTCCGACNTGCTCCCCNNGAAGNCNNGTAGNNCTTAGGTCATGCNTATGATTTTTATANANATNTTGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGAAACTCTTTGGAATTCCTTCACCNTNGACNGNTTCATANAGGCNAACCACAAGCTCANGANGAGTCNCCCTGNACAACTTCTTCAACTTCNGTTNCCTCCTCCTTTACCTCANNGANCNTCNNCTCGAGNCATCGCCNCCNGANGNAAGANGNTGTCCAAATCNATCGTGGAGGCTTGTGANGNGAACAANTNNTTCNTTACGTNGGNTNTNGANAAGAAGGAGTACAGNAGAACCCNGNTANTNNANGNTGGNAGTNCCNAAGGNGNNAGATCNGNNNNTGCCTTCAGACCCANNCCTCANNNTGAAGGTCATGGTNGNNNNTCACCTCNNNGAGACNNATCACCTCNTCGTGAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_10087446_10087552_zu-0_hi-0_22145_729773_TRUE
SEQUENCE_TARGET=950,206
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14381109_14381185_tsu-0_can-0_32249_15221_TRUE
SEQUENCE_TARGET=950,176
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_10041351_10041377_zu-0_ler-0_20947_23916_TRUE
SEQUENCE_TARGET=950,126
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNATGAATCTNGTACCTATTCAATGGNGTTANCAAGGANCACNNTACTCNAAAAGGAGCCATAGATAACCTCCTTANCGCNAAATCATTTTGGTTAATGAANGGACTATTCTGGAGAGACTTTGNNCTCTANCNTANAAACNTGGTGTTGCNAATGAAGNTNTANAGNTATATTANTGTATANTTATTGCNTTTATTTGGANTNTTTTAGAAATNAATTCATATTTNTTTTTNANTTTTGNTAACTAGATCAATGCNTCAAGATTCAANGTAGTNNATCTNATTGTTCATANAGTAGAANACTATGAAAATCTTACCAANNTTAACAAANATTTATTTCGNTNNNNNNNNNNNNNNNNNNNNGNNTTGTTGTTNNAGTNTTTNAGNCCTTTNGAAATATTGGAANAANGANGAGTANAAGANTANATTAANATGGTTATGNATTTNTAATTTATATCTCACTTNNTTGAGATAAAANNGTTNNATTNTACGCCNNAAAACCNTAAGNTGANGTTCAAGCATGTANATNNNNNNNNNNNNNNNCTGNACAATAGTTACGTTGANGNCATANCATTGTAAAATGTTAGCTNACTAGGCAAGTNNNNNNNNNNNNNNNTTTTGGAANNNNNNNNNNNNTTTTAANGGTTCTAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_14892497_14892844_oy-0_rsch-4_701553_115961_TRUE
SEQUENCE_TARGET=950,447
SEQUENCE_TEMPLATE=ATTGTGACACATTTTGATCATAGNAAAACTAACAAAGCCATTTACNNNTTNTAAGCAATTTTTTGTNGTTTTATCCTCTTTTGAGAGAAACTTGATATAAGTGTTGTCTAAACTCTTCTTATCCATCTTTAACTCTTATAATTAGTCAAATACATTAGATTGTGACACATTTTGACCATAAAAACACTAACAAAACTTTTTACTGCTTCTAAGCAATATTTTGTTGGTTTTAGCCTCTTTTGGGAGAAAATGGTTATAAGTANTANNTAAACACTCTTNNNNTATNTCTAACTCTTATAATTAGTCAAATGCATTGGATTCTGACAAATTTTGACAATAGAAACACTAACAAAGCTATTTACTCCTTCTAAGCAATTTTTTGTTGGTTTTAGCCTCTTTTGGTAGAAAATGGGTATAAGTGTTGTCTAAACACTCNTAATCCATCTNTAACTCTTACACTACAAAAATATTGGCTTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_2579586_2579629_can-0_bur-0_2907_5907_TRUE
SEQUENCE_TARGET=950,143
SEQUENCE_TEMPLATE=GGTTTTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNCNNNNNNNNNNNNNNNTGAGTCCTTCCNANGCCCANNNTTTCTGAAATTGAAGTGAGTTTCNTCCNNAATCCCTTAAAGTTTCAAANTTTTCAATNAACNTNTNNNTNTTTNTCTTGTTTNTNTCCGAATCGTNTGAGNGTTTTNATTTTCTATNCTCTGANTTGGTCTCANTCGATGTACGTTTNTAANGAATGNGTGTTCNAAATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATGAGGTACCAATTNACNGTCTTTGANTCTTTCNTNNTTGTTTTGTNATNAAGGGTTCTGAGCTTTGTNTCTTTAGGTTAATTTTCTATNTTNTNTNNNNNNNNNNNNNNNNNNNNNTAGCCAAACCAAAGANTGTGNNATCTGNATTTTNATTTNNGTNAAAAANACTGTATAGAAATGNCTTACNCTCAAGNAACTTTTATATTGCNTAAATGANTNGCTNAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAGTNTTTTNGGACANAGATGGCANNANTTCAGAAACCAGTGNTNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGCAAANACNTGCGTTTACNGAACAGGTNNNNTATANAAGTGTTCATGTATNTCTTTCTAATANTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGAATTACNTTTCTNGTTTGAGANAANGTCATNTTNGANNTGGTNAAGNNNNTAAGAACGTTGACATNTTNTGNTTNAAAANAAAAGACCTGGCTTNCATTAACTNTGCNTTGGANGCTTNCTCTAATTANTTNCTGATTAAAGAAAGACATNCCCTTANTNTNNAGNTGACCATTANACAGAACAGTAAGCTCTNNNCACNCACATGGNNNNNNNNNNNNATNNAAACGGACTAACTTGTANTCTCTNTNCCCTGTCAAGNNTTAGCAAATCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCTNTCTCTGNCCCCTCTTCTTGNTNTCANTGACNNTTNTTTTNGGTGNTTGNNNNNNTTANNNNTGTGNTTTCAAANNANNNNNNTGTNATGATNNTAGGNTTNTGATGGNNNNNNNNNNNNNNNNNCTNGATANNANTGAGGCANAGANGTCAACNGNTGTGTNTCATGACCAANNGNNANNAANANACTTCCCNATGAATNTGANAANCAAAGTCGCANGCAAACTCNGTTCNCNAGGAGGNGATNGCGCCACNNCACCTANGGNAGACANTNAATCTGGNCTAGNGTTCCNNAACAANCTCANATTNTCNGATCTTTTCNATNTCCCNNNTGAAAATGTCCANATGAATNNNNNNNNNTTTAGCGGANTGANTNATGGGNACAAAGANTGTCTGGANANNNNNNNNNNNNNTGGAACNNNAATGGCATGTCTNAAGAGCGATNNAGGAGATTCNNTNCTTCATCTTGCTGCTANATGGNGTCATCTAGAANTAGTNAAGANCNTTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGNNTCAGNTTCCNCTTCATGTGGCNGCTCNTGCNNNNNNNNNNNNNNNNNNNNANGCTCTTGTTGCNNCNNTAANATTTNTNTCAGATAGACTGGCTGAAGAAGNTANNGAGAGACTNAATCCATATGTTCTCNNNGANAAANATGGAAATANTNCTCTGCACTTGGCCATTGNAGGACGCTANNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCANNATGCTTCTTTTCTTGANAATAANNANGGAATATCTNCCTTGTANATGGCNGTAGAANCNGGAGANNTNNCNCTNGTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_12397953_12398650_hi-0_ct-1_8898_23695_TRUE
SEQUENCE_TARGET=950,797
SEQUENCE_TEMPLATE=CATGGAGAAGATCTTGTTCAGTGTCTCCCATCTTTTCTTCACAGTNCCATAACCAAAANTGAATATCTCATTGCCTCCATCACCAACTACTACTTTGAGCAACTGAAGAACATGAAGCTGTGTTTCTTTAGAAACTCCCATACTAGTTAAACGTATAAATCTNTCCATTTTTTCATAAATAGCTTTGTCCTTTACCAATCCCCACCTGCATCAAGAAAAACTTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTAAAAGTAAATNANTCACCCGAATCTTGANCCAGCATGACCTGTAGTCTTGGAGAGACTAAACAGGCTCAAATCTTCNTCAACTGGATNTGTAATAGGTGAGAAATGAGGCCAGTAATAAGCATAATCATGAAGTGTTTTCACATTAGGACCATCAAGAACCGCTCTTTTCAATTTCCCATCTGGATTATTCGGAGATNTNACNACCTCTATAACTTGTGTGATATTATCATTGNGTCCNCTCTGCTTCCACGCAGANGCATNTCCTTCAAACTTGAGATGTGCTGAATCAAANAACTCTGCTTGGTCCTTGTACATCTGCGCATACAGACTGATCAGACTCAAACATTCGATAATNTTTTACTAATCAGCAGAGGTGATGGCCGGCTTACTTACAGCATAGTATGGAATAGAAGTCAGAAGNCTTGCAGGTGATGATACTGATGAGTTTGTCAAAGACAAGGCATGAACAGCTGCTGCAAGCAATTGTGTGGTACCAGATCCAAAGATTACGAATCTGTTATCTGTAACTGCATTTCCCACTACACTATGAAGTTTTCTAATGACCTTCTCGAGCTCTCGAGACACATATGTCCCATCTTCNTANATATAACTCATCCTGTGCCATCCCGAGACCAAAANCGCGCTTCTCTCTGCTTGTNGCATCCAGAAAGGCTCCAAGAACAAAGGGTCTCCTCTTCANAATCCACNNNNNNNNNNNAATAACANNTTTATNCAAAGTTTACAGATTGGTTTGGTCATNATGATCAAGATNAAAAGTTGTTGCATACGAGTTAGCATCGACAGGACANTCTTTGAGAAGAACNGAGCAATCTTTTCCTATGTAGCAANTGTTGCACTCACAAGGAGNTTTCTGGCCGTCAAGGACACCAAGACCATCCACAAAAGCTCTGCCGTGGCCTGAGCATGAAAAAGAGGCTNCAGTTTCTGCTTCCGCTGCAGCTCTGTTGGTCCANGTTGGANTCCATGTGGTTGAANTATAATAAAGAATATGAATGGTGAATACGAGATTCAANATGATTGANACNANAAGAAGTAGCTTCTTCTGCATCATCTTTACTTAAAACCTGCNNAAAAAATTNAGCTTCAANTTTTTNTNTGTATAAAAGGTNGGGNTCAATTACAAAGGAATCAAAACAATTGTTAAATGATTCAAATATANATATTCAAGAAATTGAGAGATTAAGACTTTGATANTGACGATGACATNNNNNNNNNNNNNNNNNNNNNNNGTCCACNTAAAGTTNAANTTTCAGTAACTATCCANTGNTAGATNCNACTTAAANAACAATTTANATNNNGAANTNGNTNAACAAAAGAGAATGACATTTTTTCTAGGANAAAAANAAAGAGAATCAAGCAAAAGATACGTAAAGAATCTNNNNNNNNNNNNTTCAACAAAACNTCAAGAGACAAGAATGATGTAGACAACACNNNNNNNNNNNNNNNNNNNNNGATAGAATCTAACTGTTTAAGCATTGTGACTCTTTTGGCTTCTCTCTGAAAGAATCTGCTTTGTCTTTTCCAAAACACCAAAGAAAATTGAACCNCCAATACCTATCCACAATACCCTTGGACCCATTCCCTGTAATACAGTGTCTCAANTNTGTCAACAACTTNGCACAAACATGAGAAACTGTGTATTCCTAAAAGAGAATTGTAAGAGNAGAAATTTGNTTCACCTTCCACAAAGCTGATGAACCTTCTTCTCTTATTATNGTCTTAATGCAATCCGAAACTCCTTTATACTGAGTTCCTGAGCCCTGCNAAANTTCAAAATGAAAAGTCNGTAATATNTTACTGGAGCTTCTANNCTCCTATNAAATGAATCCANGAANAACNATTCTCTTTCAGTATTTTGTGTATTTATCGTTTTGACATACCTGAACCATTAATCTGGTTTTGATAACATCAAGAGGAGTGGTCAAAACTCCAGTGACAGCACCAGCAAAAGCACCAATCATCNCATTCTCTGGATCATTTAGATCTCTTCTTGCCTGTTCTAAACAAATGGATATCACACTGTCATTNGCCTGCAAAATCGGANGTTCTGTTACTTATCATTGTAGCAACAAAAACTTACAGCCAACTTGTATCCNATNCGTAATTGCTCATAAACACAAAACTGGAGCGCGTCAAAAGGCAAATCNCGCAATAAGAAGGATCCATAACCCTGCATGTTTAGGAACATANTACATTACATGGAGCTTTNGTTGTCTGTTCATCTTAANCTCAAGNTGTGCCTNCTNNNNNNAAAATANTAAANNACTANAACCATNAATCAANAAGTAACAGNTATACCGCATACATGCCTCCAAAGCCCTCCTTGGCTATAATAAGGCGAACAGCGTCAGGGGCCNAAACAAATTGACCAGTCTGCATCCGCTGTTTCACAACCTGTTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.602334
PRIMER_LEFT_0_PENALTY=1.483619
PRIMER_RIGHT_0_PENALTY=0.118715
PRIMER_LEFT_0_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_0_SEQUENCE=GGAATGGGTCCAAGGGTATT
PRIMER_LEFT_0=882,20
PRIMER_RIGHT_0=1877,20
PRIMER_LEFT_0_TM=58.516
PRIMER_RIGHT_0_TM=59.881
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=5.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=996
PRIMER_PAIR_1_PENALTY=1.957314
PRIMER_LEFT_1_PENALTY=1.483619
PRIMER_RIGHT_1_PENALTY=0.473695
PRIMER_LEFT_1_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_1_SEQUENCE=ATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_1=882,20
PRIMER_RIGHT_1=1874,20
PRIMER_LEFT_1_TM=58.516
PRIMER_RIGHT_1_TM=59.526
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=993
PRIMER_PAIR_2_PENALTY=2.240875
PRIMER_LEFT_2_PENALTY=2.122160
PRIMER_RIGHT_2_PENALTY=0.118715
PRIMER_LEFT_2_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_2_SEQUENCE=GGAATGGGTCCAAGGGTATT
PRIMER_LEFT_2=881,21
PRIMER_RIGHT_2=1877,20
PRIMER_LEFT_2_TM=58.878
PRIMER_RIGHT_2_TM=59.881
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=997
PRIMER_PAIR_3_PENALTY=2.379741
PRIMER_LEFT_3_PENALTY=2.261026
PRIMER_RIGHT_3_PENALTY=0.118715
PRIMER_LEFT_3_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_3_SEQUENCE=GGAATGGGTCCAAGGGTATT
PRIMER_LEFT_3=883,20
PRIMER_RIGHT_3=1877,20
PRIMER_LEFT_3_TM=62.261
PRIMER_RIGHT_3_TM=59.881
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=995
PRIMER_PAIR_4_PENALTY=2.382653
PRIMER_LEFT_4_PENALTY=1.483619
PRIMER_RIGHT_4_PENALTY=0.899034
PRIMER_LEFT_4_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_4_SEQUENCE=GAATGGGTCCAAGGGTATTG
PRIMER_LEFT_4=882,20
PRIMER_RIGHT_4=1876,20
PRIMER_LEFT_4_TM=58.516
PRIMER_RIGHT_4_TM=59.101
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=995
PRIMER_PAIR_5_PENALTY=2.551049
PRIMER_LEFT_5_PENALTY=1.483619
PRIMER_RIGHT_5_PENALTY=1.067430
PRIMER_LEFT_5_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_5_SEQUENCE=GAATGGGTCCAAGGGTATTGT
PRIMER_LEFT_5=882,20
PRIMER_RIGHT_5=1876,21
PRIMER_LEFT_5_TM=58.516
PRIMER_RIGHT_5_TM=59.933
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=995
PRIMER_PAIR_6_PENALTY=2.595854
PRIMER_LEFT_6_PENALTY=2.122160
PRIMER_RIGHT_6_PENALTY=0.473695
PRIMER_LEFT_6_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_6_SEQUENCE=ATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_6=881,21
PRIMER_RIGHT_6=1874,20
PRIMER_LEFT_6_TM=58.878
PRIMER_RIGHT_6_TM=59.526
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=994
PRIMER_PAIR_7_PENALTY=2.734720
PRIMER_LEFT_7_PENALTY=2.261026
PRIMER_RIGHT_7_PENALTY=0.473695
PRIMER_LEFT_7_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_7_SEQUENCE=ATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_7=883,20
PRIMER_RIGHT_7=1874,20
PRIMER_LEFT_7_TM=62.261
PRIMER_RIGHT_7_TM=59.526
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.3000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=992
PRIMER_PAIR_8_PENALTY=3.021194
PRIMER_LEFT_8_PENALTY=2.122160
PRIMER_RIGHT_8_PENALTY=0.899034
PRIMER_LEFT_8_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_8_SEQUENCE=GAATGGGTCCAAGGGTATTG
PRIMER_LEFT_8=881,21
PRIMER_RIGHT_8=1876,20
PRIMER_LEFT_8_TM=58.878
PRIMER_RIGHT_8_TM=59.101
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=6.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=996
PRIMER_PAIR_9_PENALTY=3.160060
PRIMER_LEFT_9_PENALTY=2.261026
PRIMER_RIGHT_9_PENALTY=0.899034
PRIMER_LEFT_9_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_9_SEQUENCE=GAATGGGTCCAAGGGTATTG
PRIMER_LEFT_9=883,20
PRIMER_RIGHT_9=1876,20
PRIMER_LEFT_9_TM=62.261
PRIMER_RIGHT_9_TM=59.101
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.3000
PRIMER_RIGHT_9_END_STABILITY=6.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=994
PRIMER_PAIR_10_PENALTY=3.188270
PRIMER_LEFT_10_PENALTY=3.069554
PRIMER_RIGHT_10_PENALTY=0.118715
PRIMER_LEFT_10_SEQUENCE=TATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_10_SEQUENCE=GGAATGGGTCCAAGGGTATT
PRIMER_LEFT_10=880,22
PRIMER_RIGHT_10=1877,20
PRIMER_LEFT_10_TM=58.930
PRIMER_RIGHT_10_TM=59.881
PRIMER_LEFT_10_GC_PERCENT=45.455
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=5.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=998
PRIMER_PAIR_11_PENALTY=3.189590
PRIMER_LEFT_11_PENALTY=2.122160
PRIMER_RIGHT_11_PENALTY=1.067430
PRIMER_LEFT_11_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_11_SEQUENCE=GAATGGGTCCAAGGGTATTGT
PRIMER_LEFT_11=881,21
PRIMER_RIGHT_11=1876,21
PRIMER_LEFT_11_TM=58.878
PRIMER_RIGHT_11_TM=59.933
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=996
PRIMER_PAIR_12_PENALTY=3.303236
PRIMER_LEFT_12_PENALTY=3.184520
PRIMER_RIGHT_12_PENALTY=0.118715
PRIMER_LEFT_12_SEQUENCE=TAACTCATCCTGTGCCATCCC
PRIMER_RIGHT_12_SEQUENCE=GGAATGGGTCCAAGGGTATT
PRIMER_LEFT_12=882,21
PRIMER_RIGHT_12=1877,20
PRIMER_LEFT_12_TM=62.185
PRIMER_RIGHT_12_TM=59.881
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.3000
PRIMER_RIGHT_12_END_STABILITY=5.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=996
PRIMER_PAIR_13_PENALTY=3.324540
PRIMER_LEFT_13_PENALTY=1.483619
PRIMER_RIGHT_13_PENALTY=1.840921
PRIMER_LEFT_13_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_13_SEQUENCE=AATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_13=882,20
PRIMER_RIGHT_13=1875,21
PRIMER_LEFT_13_TM=58.516
PRIMER_RIGHT_13_TM=60.841
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=994
PRIMER_PAIR_14_PENALTY=3.328456
PRIMER_LEFT_14_PENALTY=2.261026
PRIMER_RIGHT_14_PENALTY=1.067430
PRIMER_LEFT_14_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_14_SEQUENCE=GAATGGGTCCAAGGGTATTGT
PRIMER_LEFT_14=883,20
PRIMER_RIGHT_14=1876,21
PRIMER_LEFT_14_TM=62.261
PRIMER_RIGHT_14_TM=59.933
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.3000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=994
PRIMER_PAIR_15_PENALTY=3.386587
PRIMER_LEFT_15_PENALTY=1.483619
PRIMER_RIGHT_15_PENALTY=1.902968
PRIMER_LEFT_15_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_15_SEQUENCE=GGGTCCAAGGGTATTGTGGA
PRIMER_LEFT_15=882,20
PRIMER_RIGHT_15=1872,20
PRIMER_LEFT_15_TM=58.516
PRIMER_RIGHT_15_TM=61.903
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=991
PRIMER_PAIR_16_PENALTY=3.389592
PRIMER_LEFT_16_PENALTY=1.483619
PRIMER_RIGHT_16_PENALTY=1.905973
PRIMER_LEFT_16_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_16_SEQUENCE=AATGGGTCCAAGGGTATTGT
PRIMER_LEFT_16=882,20
PRIMER_RIGHT_16=1875,20
PRIMER_LEFT_16_TM=58.516
PRIMER_RIGHT_16_TM=58.094
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=994
PRIMER_PAIR_17_PENALTY=3.486800
PRIMER_LEFT_17_PENALTY=1.483619
PRIMER_RIGHT_17_PENALTY=2.003182
PRIMER_LEFT_17_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_17_SEQUENCE=AGCCAAAAGAGTCACAATGCT
PRIMER_LEFT_17=882,20
PRIMER_RIGHT_17=1787,21
PRIMER_LEFT_17_TM=58.516
PRIMER_RIGHT_17_TM=58.997
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=8.1000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=906
PRIMER_PAIR_18_PENALTY=3.533357
PRIMER_LEFT_18_PENALTY=1.483619
PRIMER_RIGHT_18_PENALTY=2.049738
PRIMER_LEFT_18_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_18_SEQUENCE=GCCAAAAGAGTCACAATGCT
PRIMER_LEFT_18=882,20
PRIMER_RIGHT_18=1786,20
PRIMER_LEFT_18_TM=58.516
PRIMER_RIGHT_18_TM=57.950
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=905
PRIMER_PAIR_19_PENALTY=3.543249
PRIMER_LEFT_19_PENALTY=3.069554
PRIMER_RIGHT_19_PENALTY=0.473695
PRIMER_LEFT_19_SEQUENCE=TATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_19_SEQUENCE=ATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_19=880,22
PRIMER_RIGHT_19=1874,20
PRIMER_LEFT_19_TM=58.930
PRIMER_RIGHT_19_TM=59.526
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=995
PRIMER_PAIR_20_PENALTY=3.658215
PRIMER_LEFT_20_PENALTY=3.184520
PRIMER_RIGHT_20_PENALTY=0.473695
PRIMER_LEFT_20_SEQUENCE=TAACTCATCCTGTGCCATCCC
PRIMER_RIGHT_20_SEQUENCE=ATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_20=882,21
PRIMER_RIGHT_20=1874,20
PRIMER_LEFT_20_TM=62.185
PRIMER_RIGHT_20_TM=59.526
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.3000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=993
PRIMER_PAIR_21_PENALTY=3.786237
PRIMER_LEFT_21_PENALTY=1.483619
PRIMER_RIGHT_21_PENALTY=2.302618
PRIMER_LEFT_21_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_21_SEQUENCE=AAGCCAAAAGAGTCACAATGCT
PRIMER_LEFT_21=882,20
PRIMER_RIGHT_21=1788,22
PRIMER_LEFT_21_TM=58.516
PRIMER_RIGHT_21_TM=60.303
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=907
PRIMER_PAIR_22_PENALTY=3.878675
PRIMER_LEFT_22_PENALTY=3.759959
PRIMER_RIGHT_22_PENALTY=0.118715
PRIMER_LEFT_22_SEQUENCE=ATATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_22_SEQUENCE=GGAATGGGTCCAAGGGTATT
PRIMER_LEFT_22=879,23
PRIMER_RIGHT_22=1877,20
PRIMER_LEFT_22_TM=59.240
PRIMER_RIGHT_22_TM=59.881
PRIMER_LEFT_22_GC_PERCENT=43.478
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=5.6000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=999
PRIMER_PAIR_23_PENALTY=3.890480
PRIMER_LEFT_23_PENALTY=1.483619
PRIMER_RIGHT_23_PENALTY=2.406861
PRIMER_LEFT_23_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_23_SEQUENCE=TCCAAGGGTATTGTGGATAGGT
PRIMER_LEFT_23=882,20
PRIMER_RIGHT_23=1869,22
PRIMER_LEFT_23_TM=58.516
PRIMER_RIGHT_23_TM=59.593
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=3.963081
PRIMER_LEFT_24_PENALTY=2.122160
PRIMER_RIGHT_24_PENALTY=1.840921
PRIMER_LEFT_24_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_24_SEQUENCE=AATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_24=881,21
PRIMER_RIGHT_24=1875,21
PRIMER_LEFT_24_TM=58.878
PRIMER_RIGHT_24_TM=60.841
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=995
PRIMER_PAIR_25_PENALTY=3.968588
PRIMER_LEFT_25_PENALTY=3.069554
PRIMER_RIGHT_25_PENALTY=0.899034
PRIMER_LEFT_25_SEQUENCE=TATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_25_SEQUENCE=GAATGGGTCCAAGGGTATTG
PRIMER_LEFT_25=880,22
PRIMER_RIGHT_25=1876,20
PRIMER_LEFT_25_TM=58.930
PRIMER_RIGHT_25_TM=59.101
PRIMER_LEFT_25_GC_PERCENT=45.455
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=6.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=997
PRIMER_PAIR_26_PENALTY=4.025128
PRIMER_LEFT_26_PENALTY=2.122160
PRIMER_RIGHT_26_PENALTY=1.902968
PRIMER_LEFT_26_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_26_SEQUENCE=GGGTCCAAGGGTATTGTGGA
PRIMER_LEFT_26=881,21
PRIMER_RIGHT_26=1872,20
PRIMER_LEFT_26_TM=58.878
PRIMER_RIGHT_26_TM=61.903
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.1000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=992
PRIMER_PAIR_27_PENALTY=4.028133
PRIMER_LEFT_27_PENALTY=2.122160
PRIMER_RIGHT_27_PENALTY=1.905973
PRIMER_LEFT_27_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_27_SEQUENCE=AATGGGTCCAAGGGTATTGT
PRIMER_LEFT_27=881,21
PRIMER_RIGHT_27=1875,20
PRIMER_LEFT_27_TM=58.878
PRIMER_RIGHT_27_TM=58.094
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=995
PRIMER_PAIR_28_PENALTY=4.083554
PRIMER_LEFT_28_PENALTY=3.184520
PRIMER_RIGHT_28_PENALTY=0.899034
PRIMER_LEFT_28_SEQUENCE=TAACTCATCCTGTGCCATCCC
PRIMER_RIGHT_28_SEQUENCE=GAATGGGTCCAAGGGTATTG
PRIMER_LEFT_28=882,21
PRIMER_RIGHT_28=1876,20
PRIMER_LEFT_28_TM=62.185
PRIMER_RIGHT_28_TM=59.101
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.3000
PRIMER_RIGHT_28_END_STABILITY=6.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=995
PRIMER_PAIR_29_PENALTY=4.101947
PRIMER_LEFT_29_PENALTY=2.261026
PRIMER_RIGHT_29_PENALTY=1.840921
PRIMER_LEFT_29_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_29_SEQUENCE=AATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_29=883,20
PRIMER_RIGHT_29=1875,21
PRIMER_LEFT_29_TM=62.261
PRIMER_RIGHT_29_TM=60.841
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.3000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=993
PRIMER_PAIR_30_PENALTY=4.125341
PRIMER_LEFT_30_PENALTY=2.122160
PRIMER_RIGHT_30_PENALTY=2.003182
PRIMER_LEFT_30_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_30_SEQUENCE=AGCCAAAAGAGTCACAATGCT
PRIMER_LEFT_30=881,21
PRIMER_RIGHT_30=1787,21
PRIMER_LEFT_30_TM=58.878
PRIMER_RIGHT_30_TM=58.997
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=907
PRIMER_PAIR_31_PENALTY=4.136984
PRIMER_LEFT_31_PENALTY=3.069554
PRIMER_RIGHT_31_PENALTY=1.067430
PRIMER_LEFT_31_SEQUENCE=TATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_31_SEQUENCE=GAATGGGTCCAAGGGTATTGT
PRIMER_LEFT_31=880,22
PRIMER_RIGHT_31=1876,21
PRIMER_LEFT_31_TM=58.930
PRIMER_RIGHT_31_TM=59.933
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=997
PRIMER_PAIR_32_PENALTY=4.163994
PRIMER_LEFT_32_PENALTY=2.261026
PRIMER_RIGHT_32_PENALTY=1.902968
PRIMER_LEFT_32_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_32_SEQUENCE=GGGTCCAAGGGTATTGTGGA
PRIMER_LEFT_32=883,20
PRIMER_RIGHT_32=1872,20
PRIMER_LEFT_32_TM=62.261
PRIMER_RIGHT_32_TM=61.903
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.3000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=990
PRIMER_PAIR_33_PENALTY=4.166999
PRIMER_LEFT_33_PENALTY=2.261026
PRIMER_RIGHT_33_PENALTY=1.905973
PRIMER_LEFT_33_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_33_SEQUENCE=AATGGGTCCAAGGGTATTGT
PRIMER_LEFT_33=883,20
PRIMER_RIGHT_33=1875,20
PRIMER_LEFT_33_TM=62.261
PRIMER_RIGHT_33_TM=58.094
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.3000
PRIMER_RIGHT_33_END_STABILITY=6.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=993
PRIMER_PAIR_34_PENALTY=4.171897
PRIMER_LEFT_34_PENALTY=2.122160
PRIMER_RIGHT_34_PENALTY=2.049738
PRIMER_LEFT_34_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_34_SEQUENCE=GCCAAAAGAGTCACAATGCT
PRIMER_LEFT_34=881,21
PRIMER_RIGHT_34=1786,20
PRIMER_LEFT_34_TM=58.878
PRIMER_RIGHT_34_TM=57.950
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=8.1000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=906
PRIMER_PAIR_35_PENALTY=4.233654
PRIMER_LEFT_35_PENALTY=3.759959
PRIMER_RIGHT_35_PENALTY=0.473695
PRIMER_LEFT_35_SEQUENCE=ATATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_35_SEQUENCE=ATGGGTCCAAGGGTATTGTG
PRIMER_LEFT_35=879,23
PRIMER_RIGHT_35=1874,20
PRIMER_LEFT_35_TM=59.240
PRIMER_RIGHT_35_TM=59.526
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=996
PRIMER_PAIR_36_PENALTY=4.251950
PRIMER_LEFT_36_PENALTY=3.184520
PRIMER_RIGHT_36_PENALTY=1.067430
PRIMER_LEFT_36_SEQUENCE=TAACTCATCCTGTGCCATCCC
PRIMER_RIGHT_36_SEQUENCE=GAATGGGTCCAAGGGTATTGT
PRIMER_LEFT_36=882,21
PRIMER_RIGHT_36=1876,21
PRIMER_LEFT_36_TM=62.185
PRIMER_RIGHT_36_TM=59.933
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.3000
PRIMER_RIGHT_36_END_STABILITY=6.6000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=995
PRIMER_PAIR_37_PENALTY=4.264207
PRIMER_LEFT_37_PENALTY=2.261026
PRIMER_RIGHT_37_PENALTY=2.003182
PRIMER_LEFT_37_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_37_SEQUENCE=AGCCAAAAGAGTCACAATGCT
PRIMER_LEFT_37=883,20
PRIMER_RIGHT_37=1787,21
PRIMER_LEFT_37_TM=62.261
PRIMER_RIGHT_37_TM=58.997
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.3000
PRIMER_RIGHT_37_END_STABILITY=8.1000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=905
PRIMER_PAIR_38_PENALTY=4.310763
PRIMER_LEFT_38_PENALTY=2.261026
PRIMER_RIGHT_38_PENALTY=2.049738
PRIMER_LEFT_38_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_38_SEQUENCE=GCCAAAAGAGTCACAATGCT
PRIMER_LEFT_38=883,20
PRIMER_RIGHT_38=1786,20
PRIMER_LEFT_38_TM=62.261
PRIMER_RIGHT_38_TM=57.950
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.3000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=904
PRIMER_PAIR_39_PENALTY=4.342334
PRIMER_LEFT_39_PENALTY=1.483619
PRIMER_RIGHT_39_PENALTY=2.858715
PRIMER_LEFT_39_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_39_SEQUENCE=TGGGTCCAAGGGTATTGTGG
PRIMER_LEFT_39=882,20
PRIMER_RIGHT_39=1873,20
PRIMER_LEFT_39_TM=58.516
PRIMER_RIGHT_39_TM=62.859
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.1000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=992
PRIMER_PAIR_40_PENALTY=4.424778
PRIMER_LEFT_40_PENALTY=2.122160
PRIMER_RIGHT_40_PENALTY=2.302618
PRIMER_LEFT_40_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_40_SEQUENCE=AAGCCAAAAGAGTCACAATGCT
PRIMER_LEFT_40=881,21
PRIMER_RIGHT_40=1788,22
PRIMER_LEFT_40_TM=58.878
PRIMER_RIGHT_40_TM=60.303
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=908
PRIMER_PAIR_41_PENALTY=4.453649
PRIMER_LEFT_41_PENALTY=4.334934
PRIMER_RIGHT_41_PENALTY=0.118715
PRIMER_LEFT_41_SEQUENCE=ATAACTCATCCTGTGCCATCCC
PRIMER_RIGHT_41_SEQUENCE=GGAATGGGTCCAAGGGTATT
PRIMER_LEFT_41=881,22
PRIMER_RIGHT_41=1877,20
PRIMER_LEFT_41_TM=62.335
PRIMER_RIGHT_41_TM=59.881
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.3000
PRIMER_RIGHT_41_END_STABILITY=5.6000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=997
PRIMER_PAIR_42_PENALTY=4.487262
PRIMER_LEFT_42_PENALTY=2.261026
PRIMER_RIGHT_42_PENALTY=2.226237
PRIMER_LEFT_42_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_42_SEQUENCE=TCCAAGGGTATTGTGGATAGG
PRIMER_LEFT_42=883,20
PRIMER_RIGHT_42=1869,21
PRIMER_LEFT_42_TM=62.261
PRIMER_RIGHT_42_TM=58.774
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.3000
PRIMER_RIGHT_42_END_STABILITY=7.1000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=987
PRIMER_PAIR_43_PENALTY=4.529021
PRIMER_LEFT_43_PENALTY=2.122160
PRIMER_RIGHT_43_PENALTY=2.406861
PRIMER_LEFT_43_SEQUENCE=ATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_43_SEQUENCE=TCCAAGGGTATTGTGGATAGGT
PRIMER_LEFT_43=881,21
PRIMER_RIGHT_43=1869,22
PRIMER_LEFT_43_TM=58.878
PRIMER_RIGHT_43_TM=59.593
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=989
PRIMER_PAIR_44_PENALTY=4.530590
PRIMER_LEFT_44_PENALTY=1.483619
PRIMER_RIGHT_44_PENALTY=3.046971
PRIMER_LEFT_44_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_44_SEQUENCE=GAGAGAAGCCAAAAGAGTCACAA
PRIMER_LEFT_44=882,20
PRIMER_RIGHT_44=1793,23
PRIMER_LEFT_44_TM=58.516
PRIMER_RIGHT_44_TM=60.047
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=43.478
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=912
PRIMER_PAIR_45_PENALTY=4.563644
PRIMER_LEFT_45_PENALTY=2.261026
PRIMER_RIGHT_45_PENALTY=2.302618
PRIMER_LEFT_45_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_45_SEQUENCE=AAGCCAAAAGAGTCACAATGCT
PRIMER_LEFT_45=883,20
PRIMER_RIGHT_45=1788,22
PRIMER_LEFT_45_TM=62.261
PRIMER_RIGHT_45_TM=60.303
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.3000
PRIMER_RIGHT_45_END_STABILITY=8.1000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=906
PRIMER_PAIR_46_PENALTY=4.621467
PRIMER_LEFT_46_PENALTY=1.483619
PRIMER_RIGHT_46_PENALTY=3.137848
PRIMER_LEFT_46_SEQUENCE=TAACTCATCCTGTGCCATCC
PRIMER_RIGHT_46_SEQUENCE=CAGAGAGAAGCCAAAAGAGTCA
PRIMER_LEFT_46=882,20
PRIMER_RIGHT_46=1795,22
PRIMER_LEFT_46_TM=58.516
PRIMER_RIGHT_46_TM=58.862
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.1000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=914
PRIMER_PAIR_47_PENALTY=4.658993
PRIMER_LEFT_47_PENALTY=3.759959
PRIMER_RIGHT_47_PENALTY=0.899034
PRIMER_LEFT_47_SEQUENCE=ATATAACTCATCCTGTGCCATCC
PRIMER_RIGHT_47_SEQUENCE=GAATGGGTCCAAGGGTATTG
PRIMER_LEFT_47=879,23
PRIMER_RIGHT_47=1876,20
PRIMER_LEFT_47_TM=59.240
PRIMER_RIGHT_47_TM=59.101
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=6.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=998
PRIMER_PAIR_48_PENALTY=4.667887
PRIMER_LEFT_48_PENALTY=2.261026
PRIMER_RIGHT_48_PENALTY=2.406861
PRIMER_LEFT_48_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_48_SEQUENCE=TCCAAGGGTATTGTGGATAGGT
PRIMER_LEFT_48=883,20
PRIMER_RIGHT_48=1869,22
PRIMER_LEFT_48_TM=62.261
PRIMER_RIGHT_48_TM=59.593
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.3000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=987
PRIMER_PAIR_49_PENALTY=4.667887
PRIMER_LEFT_49_PENALTY=2.261026
PRIMER_RIGHT_49_PENALTY=2.406861
PRIMER_LEFT_49_SEQUENCE=AACTCATCCTGTGCCATCCC
PRIMER_RIGHT_49_SEQUENCE=GTCCAAGGGTATTGTGGATAGG
PRIMER_LEFT_49=883,20
PRIMER_RIGHT_49=1870,22
PRIMER_LEFT_49_TM=62.261
PRIMER_RIGHT_49_TM=59.593
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.3000
PRIMER_RIGHT_49_END_STABILITY=7.1000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=988
=
SEQUENCE_ID=MAGIC.149_5_12374169_12374930_edi-0_wil-2_58195_331584_TRUE
SEQUENCE_TARGET=950,861
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTNATGNNTNCAGGGAGGAAAAGATTCATNNGTTNTNNNNNNNNNNNNNNNNNAAGGAGAGTTTCATTGTTGGGCNTAGGNGGNGTNTAGNGACCCTCGCTANNAGNTAAGNGGCCAAGNGGGAAAANATCAAGGAANGACCGAGGNANNNNNNNNNNNNAAAGAAANAGTATAGGGACGAAAGCCAAAGAGATGNGCAAGGTAAAAATGAACTTGGCCAATTCNAGTTNAAAACCGGGTCATCCNGNCNAAGCCAAAACGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGTTACCACCGAGAAACTCTGCCCGTGACTAAGANTNTTTGACCGTGAGTGAANAAATNNTGTNCGTGATTAANAATTCCTGCCCATGAGNNAGAAATCNATGCCGTTATCCTTTNCGTTCGNGAATCNGCCGAGGAACCCGTCCCATAAATGGTTATTCAGAGTAAAGCTTCATGAATCAAGGNTACTTCTATTAANAGGTGTTAGATCAAGATGAGGAGNNNNNNNNNNNNNTNGGATCAAGATTGNGAAGCCATGGAATNTNNGAGAACCGTTNAAGATAACCTTNAACCTTCAACNGAATCTNTGTGAGTCTTGAANCGTGTGANATGNCACATGATAGGTTTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_4437376_4437402_po-0_can-0_54288_54993_TRUE
SEQUENCE_TARGET=950,126
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_10908235_10908277_bur-0_wu-0_164_2711987_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=TGCAATAGCATGTCGAAATCGAGCGATGGTGTGCAAGCACGTCCTTCGCCGCAAGATTTGCTTCTTCGAAACGCAGTGGCNGCATCTTGAACGAGCATTAGGTCAAGAACTTGCTCAGTNGTTCTGTCCTTCCAGTTGTGAATAGAGCTATTTCTAGGTGACGTNGCTGCTGAGTTTGGGAGGCTGCAGCTCACAAACTTCGTCTTCATTTGATGATCNNNNNNNNNGTCATCGATGTCTCTCGGACGTGAGACTATCATATCGAAGCTCCCATTCCTNGACAATAGCGATTGCGGATCAACCGAGATCCGCCTCTCGGATACGGATTGCATACTCGTCTTCTTGATAAGAATCTTGGGCTCGTCTCCACCACCTAGAACCTCAAAGTCATGCCAGTCCTCGAGACTACTATCGTCCCTGAAGCTGAGAACATCTGTNNCCATTTGTTNCTCTTTGGCCTGNAANNGAATGAGGACTAATAAGAAAATGAAAATATTGGCAAAANGNCTAANACAGATTGATTGATACTTAAATACGGAGCAGGATGGGTTGCACGTCGGTGNCTACNTTAGTCATGACTTGCACGTTTAATATAATATAACCCAAAGTGTTGTTGACTTCATCTNCTAAAAAANGAAAAATGNNTCATNNTTTTTTTATTNGTTAANTATCNCANTATCGTTGACATGAGACCATNCCCANGATCAAGACAAGATCAAAAAGATAGATCTGCCTGCACGAACTACCTAATTATAATATTACCAAAGNATTTGGAGGGTNGTAATCTGCATCAGTGAAGACCATAAGTAGAAGCCATAAATCTTGAATACNTCTNCNNNTNNATGNACNTNGTNCTNTATNNNNTACNTNNTACTTGGNNANTTNTTTNTNNTTGGTNACNTNTNNTTNTCNTTGNTNACTNTNCNCGNNANACTTNGNTATANNNCTTNNTNATANNNCTTNTNNNTNNNTCTNNTATTTTTNNNTTNNNNTNNNTTTTNCNNNNNNNNNNNNNNNNNCTTTGAATTTTNNTGTGTCTTTTNCTACAAATATTTCTTCACTTTCATTGAAATTCATCNCCTTCAATGGATGCACNTACGTCCGTTAGTCATNNNTGGCTTTAGGCCTCATATNTATTCTAGNTGCTCTAGTATATCCCTCNAAAATGGACGGTGAGAAACAATACCAATTTTTNATCCCAAGGGATTTGAACCGGTTATGTTTGAATAACATAGNGATTTGAACGTCAAATCAACAANTTACGAAACGATTAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGCTAAAACATTTTTGAANGTTTTCAAATATGGATTTGATCGACAAANATAGAAGTCATATACTCCTTGATTTTTCCTTGGGGATTGGTGNATCCTTCATTTNCATTGTAGGNGACGNTCATCATNCGTGNAAGATCAATGGTTGCCTTAAGAAGAAAATGTGGCACGCCAGTTGTTGNCAAGAACTCCTCATTTAGTGTCTTATTGGCANCTNCAACNATTTTANNAAGCTCCCTAAAAGNTTCCTCTTTGGTAACCCCATATTGCTTCATATANCAGTTNACCGCGTTCGNGANATATCCTCTACTCATGTCNTCCTACTCAATATTTGNGTAAGTNGGTAAGATTTACAAGAAAGCAGTTATACGCGAGATGTAAAGAAANNATTTATGTATTTGTACCTCATAACCCATNAAATCATTNATAAGACGACCTTTGATAGACAAATATTGGACGAGTTTTGGTCTGGATTTCAGCCNNTCAAAAGCTTCCTTTGTNGCCATCTTTCCNATAGACATGAAAATNCCTGCCAAGGACGCATACACTGTGACCTCCACCTCACCAACCTCCATATACTNCTCAAAGCTAGGAATGTGATCNGCTTGTGNCCATTTTTCGAGATCAATGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.350503
PRIMER_LEFT_0_PENALTY=0.083883
PRIMER_RIGHT_0_PENALTY=0.266620
PRIMER_LEFT_0_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_0_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_0=529,20
PRIMER_RIGHT_0=1500,20
PRIMER_LEFT_0_TM=59.916
PRIMER_RIGHT_0_TM=59.733
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=972
PRIMER_PAIR_1_PENALTY=0.541950
PRIMER_LEFT_1_PENALTY=0.285121
PRIMER_RIGHT_1_PENALTY=0.256829
PRIMER_LEFT_1_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_1_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_1=727,20
PRIMER_RIGHT_1=1582,20
PRIMER_LEFT_1_TM=60.285
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=0.551741
PRIMER_LEFT_2_PENALTY=0.285121
PRIMER_RIGHT_2_PENALTY=0.266620
PRIMER_LEFT_2_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_2_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_2=727,20
PRIMER_RIGHT_2=1500,20
PRIMER_LEFT_2_TM=60.285
PRIMER_RIGHT_2_TM=59.733
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=774
PRIMER_PAIR_3_PENALTY=0.566115
PRIMER_LEFT_3_PENALTY=0.083883
PRIMER_RIGHT_3_PENALTY=0.482233
PRIMER_LEFT_3_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_3_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_3=529,20
PRIMER_RIGHT_3=1501,20
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=60.482
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=973
PRIMER_PAIR_4_PENALTY=0.767353
PRIMER_LEFT_4_PENALTY=0.285121
PRIMER_RIGHT_4_PENALTY=0.482233
PRIMER_LEFT_4_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_4_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_4=727,20
PRIMER_RIGHT_4=1501,20
PRIMER_LEFT_4_TM=60.285
PRIMER_RIGHT_4_TM=60.482
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=775
PRIMER_PAIR_5_PENALTY=0.769116
PRIMER_LEFT_5_PENALTY=0.512286
PRIMER_RIGHT_5_PENALTY=0.256829
PRIMER_LEFT_5_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_5_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_5=730,20
PRIMER_RIGHT_5=1582,20
PRIMER_LEFT_5_TM=59.488
PRIMER_RIGHT_5_TM=60.257
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.5000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=853
PRIMER_PAIR_6_PENALTY=0.778907
PRIMER_LEFT_6_PENALTY=0.512286
PRIMER_RIGHT_6_PENALTY=0.266620
PRIMER_LEFT_6_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_6_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_6=730,20
PRIMER_RIGHT_6=1500,20
PRIMER_LEFT_6_TM=59.488
PRIMER_RIGHT_6_TM=59.733
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=771
PRIMER_PAIR_7_PENALTY=0.979374
PRIMER_LEFT_7_PENALTY=0.712754
PRIMER_RIGHT_7_PENALTY=0.266620
PRIMER_LEFT_7_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_7_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_7=531,20
PRIMER_RIGHT_7=1500,20
PRIMER_LEFT_7_TM=60.713
PRIMER_RIGHT_7_TM=59.733
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=970
PRIMER_PAIR_8_PENALTY=0.979374
PRIMER_LEFT_8_PENALTY=0.712754
PRIMER_RIGHT_8_PENALTY=0.266620
PRIMER_LEFT_8_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_8_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_8=530,20
PRIMER_RIGHT_8=1500,20
PRIMER_LEFT_8_TM=60.713
PRIMER_RIGHT_8_TM=59.733
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=0.994519
PRIMER_LEFT_9_PENALTY=0.512286
PRIMER_RIGHT_9_PENALTY=0.482233
PRIMER_LEFT_9_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_9_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_9=730,20
PRIMER_RIGHT_9=1501,20
PRIMER_LEFT_9_TM=59.488
PRIMER_RIGHT_9_TM=60.482
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.5000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=772
PRIMER_PAIR_10_PENALTY=1.194986
PRIMER_LEFT_10_PENALTY=0.712754
PRIMER_RIGHT_10_PENALTY=0.482233
PRIMER_LEFT_10_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_10_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_10=531,20
PRIMER_RIGHT_10=1501,20
PRIMER_LEFT_10_TM=60.713
PRIMER_RIGHT_10_TM=60.482
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=971
PRIMER_PAIR_11_PENALTY=1.194986
PRIMER_LEFT_11_PENALTY=0.712754
PRIMER_RIGHT_11_PENALTY=0.482233
PRIMER_LEFT_11_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_11_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_11=530,20
PRIMER_RIGHT_11=1501,20
PRIMER_LEFT_11_TM=60.713
PRIMER_RIGHT_11_TM=60.482
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=1.227016
PRIMER_LEFT_12_PENALTY=0.083883
PRIMER_RIGHT_12_PENALTY=1.143133
PRIMER_LEFT_12_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_12_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_12=529,20
PRIMER_RIGHT_12=1496,20
PRIMER_LEFT_12_TM=59.916
PRIMER_RIGHT_12_TM=61.143
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=968
PRIMER_PAIR_13_PENALTY=1.227016
PRIMER_LEFT_13_PENALTY=0.083883
PRIMER_RIGHT_13_PENALTY=1.143133
PRIMER_LEFT_13_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_13_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_13=529,20
PRIMER_RIGHT_13=1497,20
PRIMER_LEFT_13_TM=59.916
PRIMER_RIGHT_13_TM=61.143
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=969
PRIMER_PAIR_14_PENALTY=1.239333
PRIMER_LEFT_14_PENALTY=0.083883
PRIMER_RIGHT_14_PENALTY=1.155450
PRIMER_LEFT_14_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_14_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_14=529,20
PRIMER_RIGHT_14=1186,21
PRIMER_LEFT_14_TM=59.916
PRIMER_RIGHT_14_TM=59.845
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=658
PRIMER_PAIR_15_PENALTY=1.239333
PRIMER_LEFT_15_PENALTY=0.083883
PRIMER_RIGHT_15_PENALTY=1.155450
PRIMER_LEFT_15_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_15_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_15=529,20
PRIMER_RIGHT_15=1187,21
PRIMER_LEFT_15_TM=59.916
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=659
PRIMER_PAIR_16_PENALTY=1.252490
PRIMER_LEFT_16_PENALTY=0.995660
PRIMER_RIGHT_16_PENALTY=0.256829
PRIMER_LEFT_16_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_16_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_16=728,20
PRIMER_RIGHT_16=1582,20
PRIMER_LEFT_16_TM=60.996
PRIMER_RIGHT_16_TM=60.257
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=855
PRIMER_PAIR_17_PENALTY=1.262281
PRIMER_LEFT_17_PENALTY=0.995660
PRIMER_RIGHT_17_PENALTY=0.266620
PRIMER_LEFT_17_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_17_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_17=728,20
PRIMER_RIGHT_17=1500,20
PRIMER_LEFT_17_TM=60.996
PRIMER_RIGHT_17_TM=59.733
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=773
PRIMER_PAIR_18_PENALTY=1.428253
PRIMER_LEFT_18_PENALTY=0.285121
PRIMER_RIGHT_18_PENALTY=1.143133
PRIMER_LEFT_18_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_18_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_18=727,20
PRIMER_RIGHT_18=1496,20
PRIMER_LEFT_18_TM=60.285
PRIMER_RIGHT_18_TM=61.143
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=770
PRIMER_PAIR_19_PENALTY=1.428253
PRIMER_LEFT_19_PENALTY=0.285121
PRIMER_RIGHT_19_PENALTY=1.143133
PRIMER_LEFT_19_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_19_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_19=727,20
PRIMER_RIGHT_19=1497,20
PRIMER_LEFT_19_TM=60.285
PRIMER_RIGHT_19_TM=61.143
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=771
PRIMER_PAIR_20_PENALTY=1.440571
PRIMER_LEFT_20_PENALTY=0.285121
PRIMER_RIGHT_20_PENALTY=1.155450
PRIMER_LEFT_20_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_20_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_20=727,20
PRIMER_RIGHT_20=1186,21
PRIMER_LEFT_20_TM=60.285
PRIMER_RIGHT_20_TM=59.845
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=460
PRIMER_PAIR_21_PENALTY=1.440571
PRIMER_LEFT_21_PENALTY=0.285121
PRIMER_RIGHT_21_PENALTY=1.155450
PRIMER_LEFT_21_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_21_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_21=727,20
PRIMER_RIGHT_21=1187,21
PRIMER_LEFT_21_TM=60.285
PRIMER_RIGHT_21_TM=59.845
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=461
PRIMER_PAIR_22_PENALTY=1.465064
PRIMER_LEFT_22_PENALTY=0.083883
PRIMER_RIGHT_22_PENALTY=1.381181
PRIMER_LEFT_22_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_22_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_22=529,20
PRIMER_RIGHT_22=1495,20
PRIMER_LEFT_22_TM=59.916
PRIMER_RIGHT_22_TM=58.619
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=967
PRIMER_PAIR_23_PENALTY=1.477893
PRIMER_LEFT_23_PENALTY=0.995660
PRIMER_RIGHT_23_PENALTY=0.482233
PRIMER_LEFT_23_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_23_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_23=728,20
PRIMER_RIGHT_23=1501,20
PRIMER_LEFT_23_TM=60.996
PRIMER_RIGHT_23_TM=60.482
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=774
PRIMER_PAIR_24_PENALTY=1.581829
PRIMER_LEFT_24_PENALTY=0.426379
PRIMER_RIGHT_24_PENALTY=1.155450
PRIMER_LEFT_24_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_24_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_24=361,20
PRIMER_RIGHT_24=1186,21
PRIMER_LEFT_24_TM=59.574
PRIMER_RIGHT_24_TM=59.845
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=826
PRIMER_PAIR_25_PENALTY=1.581829
PRIMER_LEFT_25_PENALTY=0.426379
PRIMER_RIGHT_25_PENALTY=1.155450
PRIMER_LEFT_25_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_25_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_25=361,20
PRIMER_RIGHT_25=1187,21
PRIMER_LEFT_25_TM=59.574
PRIMER_RIGHT_25_TM=59.845
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=827
PRIMER_PAIR_26_PENALTY=1.637322
PRIMER_LEFT_26_PENALTY=0.083883
PRIMER_RIGHT_26_PENALTY=1.553439
PRIMER_LEFT_26_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_26_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_26=529,20
PRIMER_RIGHT_26=1495,21
PRIMER_LEFT_26_TM=59.916
PRIMER_RIGHT_26_TM=59.447
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=967
PRIMER_PAIR_27_PENALTY=1.655419
PRIMER_LEFT_27_PENALTY=0.512286
PRIMER_RIGHT_27_PENALTY=1.143133
PRIMER_LEFT_27_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_27_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_27=730,20
PRIMER_RIGHT_27=1496,20
PRIMER_LEFT_27_TM=59.488
PRIMER_RIGHT_27_TM=61.143
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.5000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=767
PRIMER_PAIR_28_PENALTY=1.655419
PRIMER_LEFT_28_PENALTY=0.512286
PRIMER_RIGHT_28_PENALTY=1.143133
PRIMER_LEFT_28_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_28_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_28=730,20
PRIMER_RIGHT_28=1497,20
PRIMER_LEFT_28_TM=59.488
PRIMER_RIGHT_28_TM=61.143
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.5000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=768
PRIMER_PAIR_29_PENALTY=1.666302
PRIMER_LEFT_29_PENALTY=0.285121
PRIMER_RIGHT_29_PENALTY=1.381181
PRIMER_LEFT_29_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_29_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_29=727,20
PRIMER_RIGHT_29=1495,20
PRIMER_LEFT_29_TM=60.285
PRIMER_RIGHT_29_TM=58.619
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=769
PRIMER_PAIR_30_PENALTY=1.667737
PRIMER_LEFT_30_PENALTY=0.512286
PRIMER_RIGHT_30_PENALTY=1.155450
PRIMER_LEFT_30_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_30_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_30=730,20
PRIMER_RIGHT_30=1186,21
PRIMER_LEFT_30_TM=59.488
PRIMER_RIGHT_30_TM=59.845
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.5000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=457
PRIMER_PAIR_31_PENALTY=1.667737
PRIMER_LEFT_31_PENALTY=0.512286
PRIMER_RIGHT_31_PENALTY=1.155450
PRIMER_LEFT_31_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_31_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_31=730,20
PRIMER_RIGHT_31=1187,21
PRIMER_LEFT_31_TM=59.488
PRIMER_RIGHT_31_TM=59.845
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=458
PRIMER_PAIR_32_PENALTY=1.669226
PRIMER_LEFT_32_PENALTY=0.513776
PRIMER_RIGHT_32_PENALTY=1.155450
PRIMER_LEFT_32_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_32_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_32=257,20
PRIMER_RIGHT_32=1186,21
PRIMER_LEFT_32_TM=59.486
PRIMER_RIGHT_32_TM=59.845
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=930
PRIMER_PAIR_33_PENALTY=1.669226
PRIMER_LEFT_33_PENALTY=0.513776
PRIMER_RIGHT_33_PENALTY=1.155450
PRIMER_LEFT_33_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_33_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_33=257,20
PRIMER_RIGHT_33=1187,21
PRIMER_LEFT_33_TM=59.486
PRIMER_RIGHT_33_TM=59.845
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=1.703379
PRIMER_LEFT_34_PENALTY=1.446550
PRIMER_RIGHT_34_PENALTY=0.256829
PRIMER_LEFT_34_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_34_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_34=729,21
PRIMER_RIGHT_34=1582,20
PRIMER_LEFT_34_TM=60.447
PRIMER_RIGHT_34_TM=60.257
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=854
PRIMER_PAIR_35_PENALTY=1.713170
PRIMER_LEFT_35_PENALTY=1.446550
PRIMER_RIGHT_35_PENALTY=0.266620
PRIMER_LEFT_35_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_35_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_35=729,21
PRIMER_RIGHT_35=1500,20
PRIMER_LEFT_35_TM=60.447
PRIMER_RIGHT_35_TM=59.733
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.5000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=772
PRIMER_PAIR_36_PENALTY=1.740964
PRIMER_LEFT_36_PENALTY=1.484135
PRIMER_RIGHT_36_PENALTY=0.256829
PRIMER_LEFT_36_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_36_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_36=725,21
PRIMER_RIGHT_36=1582,20
PRIMER_LEFT_36_TM=59.516
PRIMER_RIGHT_36_TM=60.257
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.1000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=858
PRIMER_PAIR_37_PENALTY=1.750755
PRIMER_LEFT_37_PENALTY=1.484135
PRIMER_RIGHT_37_PENALTY=0.266620
PRIMER_LEFT_37_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_37_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_37=725,21
PRIMER_RIGHT_37=1500,20
PRIMER_LEFT_37_TM=59.516
PRIMER_RIGHT_37_TM=59.733
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.1000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=776
PRIMER_PAIR_38_PENALTY=1.785200
PRIMER_LEFT_38_PENALTY=1.528370
PRIMER_RIGHT_38_PENALTY=0.256829
PRIMER_LEFT_38_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_38_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_38=726,20
PRIMER_RIGHT_38=1582,20
PRIMER_LEFT_38_TM=58.472
PRIMER_RIGHT_38_TM=60.257
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.1000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=857
PRIMER_PAIR_39_PENALTY=1.794991
PRIMER_LEFT_39_PENALTY=1.528370
PRIMER_RIGHT_39_PENALTY=0.266620
PRIMER_LEFT_39_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_39_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_39=726,20
PRIMER_RIGHT_39=1500,20
PRIMER_LEFT_39_TM=58.472
PRIMER_RIGHT_39_TM=59.733
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.1000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=775
PRIMER_PAIR_40_PENALTY=1.811843
PRIMER_LEFT_40_PENALTY=0.656393
PRIMER_RIGHT_40_PENALTY=1.155450
PRIMER_LEFT_40_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_40_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_40=379,20
PRIMER_RIGHT_40=1186,21
PRIMER_LEFT_40_TM=60.656
PRIMER_RIGHT_40_TM=59.845
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=808
PRIMER_PAIR_41_PENALTY=1.811843
PRIMER_LEFT_41_PENALTY=0.656393
PRIMER_RIGHT_41_PENALTY=1.155450
PRIMER_LEFT_41_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_41_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_41=379,20
PRIMER_RIGHT_41=1187,21
PRIMER_LEFT_41_TM=60.656
PRIMER_RIGHT_41_TM=59.845
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=809
PRIMER_PAIR_42_PENALTY=1.838560
PRIMER_LEFT_42_PENALTY=0.285121
PRIMER_RIGHT_42_PENALTY=1.553439
PRIMER_LEFT_42_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_42_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_42=727,20
PRIMER_RIGHT_42=1495,21
PRIMER_LEFT_42_TM=60.285
PRIMER_RIGHT_42_TM=59.447
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=769
PRIMER_PAIR_43_PENALTY=1.855887
PRIMER_LEFT_43_PENALTY=0.712754
PRIMER_RIGHT_43_PENALTY=1.143133
PRIMER_LEFT_43_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_43_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_43=531,20
PRIMER_RIGHT_43=1496,20
PRIMER_LEFT_43_TM=60.713
PRIMER_RIGHT_43_TM=61.143
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=1.855887
PRIMER_LEFT_44_PENALTY=0.712754
PRIMER_RIGHT_44_PENALTY=1.143133
PRIMER_LEFT_44_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_44_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_44=531,20
PRIMER_RIGHT_44=1497,20
PRIMER_LEFT_44_TM=60.713
PRIMER_RIGHT_44_TM=61.143
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=967
PRIMER_PAIR_45_PENALTY=1.855887
PRIMER_LEFT_45_PENALTY=0.712754
PRIMER_RIGHT_45_PENALTY=1.143133
PRIMER_LEFT_45_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_45_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_45=530,20
PRIMER_RIGHT_45=1496,20
PRIMER_LEFT_45_TM=60.713
PRIMER_RIGHT_45_TM=61.143
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=967
PRIMER_PAIR_46_PENALTY=1.855887
PRIMER_LEFT_46_PENALTY=0.712754
PRIMER_RIGHT_46_PENALTY=1.143133
PRIMER_LEFT_46_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_46_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_46=530,20
PRIMER_RIGHT_46=1497,20
PRIMER_LEFT_46_TM=60.713
PRIMER_RIGHT_46_TM=61.143
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=968
PRIMER_PAIR_47_PENALTY=1.868204
PRIMER_LEFT_47_PENALTY=0.712754
PRIMER_RIGHT_47_PENALTY=1.155450
PRIMER_LEFT_47_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_47_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_47=531,20
PRIMER_RIGHT_47=1186,21
PRIMER_LEFT_47_TM=60.713
PRIMER_RIGHT_47_TM=59.845
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=656
PRIMER_PAIR_48_PENALTY=1.868204
PRIMER_LEFT_48_PENALTY=0.712754
PRIMER_RIGHT_48_PENALTY=1.155450
PRIMER_LEFT_48_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_48_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_48=531,20
PRIMER_RIGHT_48=1187,21
PRIMER_LEFT_48_TM=60.713
PRIMER_RIGHT_48_TM=59.845
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=657
PRIMER_PAIR_49_PENALTY=1.868204
PRIMER_LEFT_49_PENALTY=0.712754
PRIMER_RIGHT_49_PENALTY=1.155450
PRIMER_LEFT_49_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_49_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_49=530,20
PRIMER_RIGHT_49=1186,21
PRIMER_LEFT_49_TM=60.713
PRIMER_RIGHT_49_TM=59.845
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=657
=
SEQUENCE_ID=MAGIC.149_1_11133893_11133919_no-0_kn-0_804092_167425_TRUE
SEQUENCE_TARGET=950,126
SEQUENCE_TEMPLATE=NCANAAGCTTGACCAAGGCAAGANAAGTATGCTTNTTATGANACCCTTCGCCTTCACAGTAGGAGGCTTCCAAGGCACCAGAGCTACTNATTAAACCATCAAAATCNAAACNCTAANAAAANAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATCATCATCCATGGGAATCGTCATATCCTCCTCCTCGTCAAGGTTGTTCCAAATTTCGACAGAAACNAGCTGACGATGCATTTTCGNTCGACTTTTGNTTTTTTTNCNGGCCNTGCNNNNNNNNNNNNNNNNNNCATCTGTTTGGGTGGGCTTAANTTGGGCTTTCTCNACCCAAANCTCGTTNACAAATANTTTTAACGAAACGACGCCNNATTTCGAGTTAATNGNCATACGTCGTCGNTTTNACTCTNTATATTTCGTTCACAAATNTTTTNAAATNAATATNATTTTTANAGACGAGAGGAGACGGCGACAATGGCGAATTTGATGATGAGATTACCAATTAGCTTGAGAAGCTTCTCTGTTTCAGCTTCTTCATCNAACGGTAACGCTNTTAAGCTCTAATNCTTNCACAGTAATCTGAAATTTAAGCNTGAAATTGAAGCTGAGNNTCTGNGATTGNTTTTTNATTTCNAGGTTCNCCGCCGGTGATCGGAGGATCTAGCGGCGGNGTAGGACCGATGATTGTGGAATTACCGTTGGANAAGATACGAAGACCGTTGATGCGAACCAGATCCAACGATCAGAACAAAGTGAAAGAGCTTATGGATAGTATCCGTCAAATCGGTCTTCAAGTTCCGGTTAAGCAACTCNGTTATTCAATTCTGANTTCAAANTTCTTCGNTTNANAANNNNNNNNNNNNNNNNNNNNNNTGANCAGATTGATGTNATTGANGTTGATGGAACTTACTATGGTANTTTTCTTGTTCTTNGTGATNTCTGTTCTTTGAAAGCTNATANATGATAANTTCTTCCATTGGTTTAGGGTTCTCGGGATGTCACAGATACGAGGCGCATCAGAAGCTAGGNCTTCCAACNATACGTTGCAAAATNCGTAAAGGAACAAAGGAAACATTAAGGTATGTATGTGTTTCTTTAAAGAATGAAAANTTNGGATTGGAGACTTTAGATTTGCTTTATATTGAAGGTTCTTTGGTTTCTCACAGGCATCATCTTCGCTGAAGAGAGTTTNTACTTTTATGTATATTGTTTGTATGATAGGAACTCNNGAGTNTGTGTATGANNNNNNNNNNNNNNAAGTTAANAATNAGAACTTTGTACAANCTTTGTGANAGATNTAGTGACTGGATTCTACNATCTATNTGCACNNNNNTAAGTAGCNGAAACAANGGTTNAAANNATTTGATCATAAATTGGNCTTNTGATGNNCGAGATGATNAGTANAANGAAGCTGTNTTCCTTATAGCTTGATNGATTACTTTGAAGATCTAAGCAGCGTTNCGNTGNCNTTTTGTATGAACNTTATTCTTCTGTTATCGGTTAAGCTTTGTGGANAGGNTTCTTTGGGNCAAGAATCTATCTATCCTCTTTACAGAACACTAATGANTCATGNTCCGCATATGTTGGACGCAATAGAAAGAAGTGGGCTGCTGATTGCGNCCATGCCTAAGGGAGGTTAGAAGCTNTGNAGTGGTATGTGATTACTTCTTNATTTCTTTAGTAATNCTTCTTTGGCCGATTGATACCAGNAAGAGAAAGAGNGAAATGCTATAAGAACCATGTAACGNTAAGAGAGAANCNTNTTTCNGATTAGTATAGGTGACGCATGACNTCGAGGCGAGGTNTACNTCGGTAACTCGAAGGGAACTCNTANATTTGGATGTTCTTTNTNTTANTCNGCATTCNNTATCN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.166764
PRIMER_LEFT_0_PENALTY=0.109200
PRIMER_RIGHT_0_PENALTY=0.057564
PRIMER_LEFT_0_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_0_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_0=626,20
PRIMER_RIGHT_0=1331,20
PRIMER_LEFT_0_TM=59.891
PRIMER_RIGHT_0_TM=59.942
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=706
PRIMER_PAIR_1_PENALTY=0.349057
PRIMER_LEFT_1_PENALTY=0.109200
PRIMER_RIGHT_1_PENALTY=0.239857
PRIMER_LEFT_1_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_1_SEQUENCE=TGATGCCTGTGAGAAACCAA
PRIMER_LEFT_1=626,20
PRIMER_RIGHT_1=1325,20
PRIMER_LEFT_1_TM=59.891
PRIMER_RIGHT_1_TM=60.240
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=700
PRIMER_PAIR_2_PENALTY=0.360385
PRIMER_LEFT_2_PENALTY=0.302821
PRIMER_RIGHT_2_PENALTY=0.057564
PRIMER_LEFT_2_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_2_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_2=343,20
PRIMER_RIGHT_2=1331,20
PRIMER_LEFT_2_TM=59.697
PRIMER_RIGHT_2_TM=59.942
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=989
PRIMER_PAIR_3_PENALTY=0.360385
PRIMER_LEFT_3_PENALTY=0.302821
PRIMER_RIGHT_3_PENALTY=0.057564
PRIMER_LEFT_3_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_3_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_3=342,20
PRIMER_RIGHT_3=1331,20
PRIMER_LEFT_3_TM=59.697
PRIMER_RIGHT_3_TM=59.942
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=990
PRIMER_PAIR_4_PENALTY=0.360385
PRIMER_LEFT_4_PENALTY=0.302821
PRIMER_RIGHT_4_PENALTY=0.057564
PRIMER_LEFT_4_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_4_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_4=341,20
PRIMER_RIGHT_4=1331,20
PRIMER_LEFT_4_TM=59.697
PRIMER_RIGHT_4_TM=59.942
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=991
PRIMER_PAIR_5_PENALTY=0.542678
PRIMER_LEFT_5_PENALTY=0.302821
PRIMER_RIGHT_5_PENALTY=0.239857
PRIMER_LEFT_5_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_5_SEQUENCE=TGATGCCTGTGAGAAACCAA
PRIMER_LEFT_5=343,20
PRIMER_RIGHT_5=1325,20
PRIMER_LEFT_5_TM=59.697
PRIMER_RIGHT_5_TM=60.240
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=983
PRIMER_PAIR_6_PENALTY=0.542678
PRIMER_LEFT_6_PENALTY=0.302821
PRIMER_RIGHT_6_PENALTY=0.239857
PRIMER_LEFT_6_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_6_SEQUENCE=TGATGCCTGTGAGAAACCAA
PRIMER_LEFT_6=342,20
PRIMER_RIGHT_6=1325,20
PRIMER_LEFT_6_TM=59.697
PRIMER_RIGHT_6_TM=60.240
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=984
PRIMER_PAIR_7_PENALTY=0.542678
PRIMER_LEFT_7_PENALTY=0.302821
PRIMER_RIGHT_7_PENALTY=0.239857
PRIMER_LEFT_7_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_7_SEQUENCE=TGATGCCTGTGAGAAACCAA
PRIMER_LEFT_7=341,20
PRIMER_RIGHT_7=1325,20
PRIMER_LEFT_7_TM=59.697
PRIMER_RIGHT_7_TM=60.240
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=985
PRIMER_PAIR_8_PENALTY=0.561019
PRIMER_LEFT_8_PENALTY=0.109200
PRIMER_RIGHT_8_PENALTY=0.451819
PRIMER_LEFT_8_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_8_SEQUENCE=TGACATCCCGAGAACCCTAA
PRIMER_LEFT_8=626,20
PRIMER_RIGHT_8=1154,20
PRIMER_LEFT_8_TM=59.891
PRIMER_RIGHT_8_TM=60.452
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.5000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=529
PRIMER_PAIR_9_PENALTY=0.694416
PRIMER_LEFT_9_PENALTY=0.109200
PRIMER_RIGHT_9_PENALTY=0.585216
PRIMER_LEFT_9_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_9_SEQUENCE=TGCCTGTGAGAAACCAAAGA
PRIMER_LEFT_9=626,20
PRIMER_RIGHT_9=1322,20
PRIMER_LEFT_9_TM=59.891
PRIMER_RIGHT_9_TM=59.415
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=697
PRIMER_PAIR_10_PENALTY=0.742878
PRIMER_LEFT_10_PENALTY=0.291059
PRIMER_RIGHT_10_PENALTY=0.451819
PRIMER_LEFT_10_SEQUENCE=TGGGAATCGTCATATCCTCC
PRIMER_RIGHT_10_SEQUENCE=TGACATCCCGAGAACCCTAA
PRIMER_LEFT_10=324,20
PRIMER_RIGHT_10=1154,20
PRIMER_LEFT_10_TM=59.709
PRIMER_RIGHT_10_TM=60.452
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=7.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=831
PRIMER_PAIR_11_PENALTY=0.754640
PRIMER_LEFT_11_PENALTY=0.302821
PRIMER_RIGHT_11_PENALTY=0.451819
PRIMER_LEFT_11_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_11_SEQUENCE=TGACATCCCGAGAACCCTAA
PRIMER_LEFT_11=343,20
PRIMER_RIGHT_11=1154,20
PRIMER_LEFT_11_TM=59.697
PRIMER_RIGHT_11_TM=60.452
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=812
PRIMER_PAIR_12_PENALTY=0.754640
PRIMER_LEFT_12_PENALTY=0.302821
PRIMER_RIGHT_12_PENALTY=0.451819
PRIMER_LEFT_12_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_12_SEQUENCE=TGACATCCCGAGAACCCTAA
PRIMER_LEFT_12=342,20
PRIMER_RIGHT_12=1154,20
PRIMER_LEFT_12_TM=59.697
PRIMER_RIGHT_12_TM=60.452
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=813
PRIMER_PAIR_13_PENALTY=0.754640
PRIMER_LEFT_13_PENALTY=0.302821
PRIMER_RIGHT_13_PENALTY=0.451819
PRIMER_LEFT_13_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_13_SEQUENCE=TGACATCCCGAGAACCCTAA
PRIMER_LEFT_13=341,20
PRIMER_RIGHT_13=1154,20
PRIMER_LEFT_13_TM=59.697
PRIMER_RIGHT_13_TM=60.452
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=814
PRIMER_PAIR_14_PENALTY=0.792462
PRIMER_LEFT_14_PENALTY=0.109200
PRIMER_RIGHT_14_PENALTY=0.683262
PRIMER_LEFT_14_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_14_SEQUENCE=ATCCCGAGAACCCTAAACCA
PRIMER_LEFT_14=626,20
PRIMER_RIGHT_14=1150,20
PRIMER_LEFT_14_TM=59.891
PRIMER_RIGHT_14_TM=60.683
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=525
PRIMER_PAIR_15_PENALTY=0.792462
PRIMER_LEFT_15_PENALTY=0.109200
PRIMER_RIGHT_15_PENALTY=0.683262
PRIMER_LEFT_15_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_15_SEQUENCE=CATCCCGAGAACCCTAAACC
PRIMER_LEFT_15=626,20
PRIMER_RIGHT_15=1151,20
PRIMER_LEFT_15_TM=59.891
PRIMER_RIGHT_15_TM=60.683
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=526
PRIMER_PAIR_16_PENALTY=0.876275
PRIMER_LEFT_16_PENALTY=0.291059
PRIMER_RIGHT_16_PENALTY=0.585216
PRIMER_LEFT_16_SEQUENCE=TGGGAATCGTCATATCCTCC
PRIMER_RIGHT_16_SEQUENCE=TGCCTGTGAGAAACCAAAGA
PRIMER_LEFT_16=324,20
PRIMER_RIGHT_16=1322,20
PRIMER_LEFT_16_TM=59.709
PRIMER_RIGHT_16_TM=59.415
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=999
PRIMER_PAIR_17_PENALTY=0.888037
PRIMER_LEFT_17_PENALTY=0.302821
PRIMER_RIGHT_17_PENALTY=0.585216
PRIMER_LEFT_17_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_17_SEQUENCE=TGCCTGTGAGAAACCAAAGA
PRIMER_LEFT_17=343,20
PRIMER_RIGHT_17=1322,20
PRIMER_LEFT_17_TM=59.697
PRIMER_RIGHT_17_TM=59.415
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=980
PRIMER_PAIR_18_PENALTY=0.888037
PRIMER_LEFT_18_PENALTY=0.302821
PRIMER_RIGHT_18_PENALTY=0.585216
PRIMER_LEFT_18_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_18_SEQUENCE=TGCCTGTGAGAAACCAAAGA
PRIMER_LEFT_18=342,20
PRIMER_RIGHT_18=1322,20
PRIMER_LEFT_18_TM=59.697
PRIMER_RIGHT_18_TM=59.415
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=981
PRIMER_PAIR_19_PENALTY=0.888037
PRIMER_LEFT_19_PENALTY=0.302821
PRIMER_RIGHT_19_PENALTY=0.585216
PRIMER_LEFT_19_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_19_SEQUENCE=TGCCTGTGAGAAACCAAAGA
PRIMER_LEFT_19=341,20
PRIMER_RIGHT_19=1322,20
PRIMER_LEFT_19_TM=59.697
PRIMER_RIGHT_19_TM=59.415
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=982
PRIMER_PAIR_20_PENALTY=0.974321
PRIMER_LEFT_20_PENALTY=0.291059
PRIMER_RIGHT_20_PENALTY=0.683262
PRIMER_LEFT_20_SEQUENCE=TGGGAATCGTCATATCCTCC
PRIMER_RIGHT_20_SEQUENCE=ATCCCGAGAACCCTAAACCA
PRIMER_LEFT_20=324,20
PRIMER_RIGHT_20=1150,20
PRIMER_LEFT_20_TM=59.709
PRIMER_RIGHT_20_TM=60.683
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=827
PRIMER_PAIR_21_PENALTY=0.974321
PRIMER_LEFT_21_PENALTY=0.291059
PRIMER_RIGHT_21_PENALTY=0.683262
PRIMER_LEFT_21_SEQUENCE=TGGGAATCGTCATATCCTCC
PRIMER_RIGHT_21_SEQUENCE=CATCCCGAGAACCCTAAACC
PRIMER_LEFT_21=324,20
PRIMER_RIGHT_21=1151,20
PRIMER_LEFT_21_TM=59.709
PRIMER_RIGHT_21_TM=60.683
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=828
PRIMER_PAIR_22_PENALTY=0.986083
PRIMER_LEFT_22_PENALTY=0.302821
PRIMER_RIGHT_22_PENALTY=0.683262
PRIMER_LEFT_22_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_22_SEQUENCE=ATCCCGAGAACCCTAAACCA
PRIMER_LEFT_22=343,20
PRIMER_RIGHT_22=1150,20
PRIMER_LEFT_22_TM=59.697
PRIMER_RIGHT_22_TM=60.683
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=808
PRIMER_PAIR_23_PENALTY=0.986083
PRIMER_LEFT_23_PENALTY=0.302821
PRIMER_RIGHT_23_PENALTY=0.683262
PRIMER_LEFT_23_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_23_SEQUENCE=CATCCCGAGAACCCTAAACC
PRIMER_LEFT_23=343,20
PRIMER_RIGHT_23=1151,20
PRIMER_LEFT_23_TM=59.697
PRIMER_RIGHT_23_TM=60.683
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=809
PRIMER_PAIR_24_PENALTY=0.986083
PRIMER_LEFT_24_PENALTY=0.302821
PRIMER_RIGHT_24_PENALTY=0.683262
PRIMER_LEFT_24_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_24_SEQUENCE=ATCCCGAGAACCCTAAACCA
PRIMER_LEFT_24=342,20
PRIMER_RIGHT_24=1150,20
PRIMER_LEFT_24_TM=59.697
PRIMER_RIGHT_24_TM=60.683
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=809
PRIMER_PAIR_25_PENALTY=0.986083
PRIMER_LEFT_25_PENALTY=0.302821
PRIMER_RIGHT_25_PENALTY=0.683262
PRIMER_LEFT_25_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_25_SEQUENCE=CATCCCGAGAACCCTAAACC
PRIMER_LEFT_25=342,20
PRIMER_RIGHT_25=1151,20
PRIMER_LEFT_25_TM=59.697
PRIMER_RIGHT_25_TM=60.683
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=810
PRIMER_PAIR_26_PENALTY=0.986083
PRIMER_LEFT_26_PENALTY=0.302821
PRIMER_RIGHT_26_PENALTY=0.683262
PRIMER_LEFT_26_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_26_SEQUENCE=ATCCCGAGAACCCTAAACCA
PRIMER_LEFT_26=341,20
PRIMER_RIGHT_26=1150,20
PRIMER_LEFT_26_TM=59.697
PRIMER_RIGHT_26_TM=60.683
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=810
PRIMER_PAIR_27_PENALTY=0.986083
PRIMER_LEFT_27_PENALTY=0.302821
PRIMER_RIGHT_27_PENALTY=0.683262
PRIMER_LEFT_27_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_27_SEQUENCE=CATCCCGAGAACCCTAAACC
PRIMER_LEFT_27=341,20
PRIMER_RIGHT_27=1151,20
PRIMER_LEFT_27_TM=59.697
PRIMER_RIGHT_27_TM=60.683
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=811
PRIMER_PAIR_28_PENALTY=1.019502
PRIMER_LEFT_28_PENALTY=0.109200
PRIMER_RIGHT_28_PENALTY=0.910302
PRIMER_LEFT_28_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_28_SEQUENCE=ATGATGCCTGTGAGAAACCA
PRIMER_LEFT_28=626,20
PRIMER_RIGHT_28=1326,20
PRIMER_LEFT_28_TM=59.891
PRIMER_RIGHT_28_TM=59.090
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=701
PRIMER_PAIR_29_PENALTY=1.124333
PRIMER_LEFT_29_PENALTY=0.884476
PRIMER_RIGHT_29_PENALTY=0.239857
PRIMER_LEFT_29_SEQUENCE=CGTCATATCCTCCTCCTCGT
PRIMER_RIGHT_29_SEQUENCE=TGATGCCTGTGAGAAACCAA
PRIMER_LEFT_29=331,20
PRIMER_RIGHT_29=1325,20
PRIMER_LEFT_29_TM=59.116
PRIMER_RIGHT_29_TM=60.240
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=995
PRIMER_PAIR_30_PENALTY=1.127912
PRIMER_LEFT_30_PENALTY=0.109200
PRIMER_RIGHT_30_PENALTY=1.018712
PRIMER_LEFT_30_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_30_SEQUENCE=GACATCCCGAGAACCCTAAA
PRIMER_LEFT_30=626,20
PRIMER_RIGHT_30=1153,20
PRIMER_LEFT_30_TM=59.891
PRIMER_RIGHT_30_TM=58.981
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=6.3000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=528
PRIMER_PAIR_31_PENALTY=1.134283
PRIMER_LEFT_31_PENALTY=1.076720
PRIMER_RIGHT_31_PENALTY=0.057564
PRIMER_LEFT_31_SEQUENCE=TAGGACCGATGATTGTGGAA
PRIMER_RIGHT_31_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_31=826,20
PRIMER_RIGHT_31=1331,20
PRIMER_LEFT_31_TM=58.923
PRIMER_RIGHT_31_TM=59.942
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=506
PRIMER_PAIR_32_PENALTY=1.191950
PRIMER_LEFT_32_PENALTY=0.109200
PRIMER_RIGHT_32_PENALTY=1.082750
PRIMER_LEFT_32_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_32_SEQUENCE=TCTGTGACATCCCGAGAACC
PRIMER_LEFT_32=626,20
PRIMER_RIGHT_32=1158,20
PRIMER_LEFT_32_TM=59.891
PRIMER_RIGHT_32_TM=61.083
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=533
PRIMER_PAIR_33_PENALTY=1.210460
PRIMER_LEFT_33_PENALTY=1.076720
PRIMER_RIGHT_33_PENALTY=0.133741
PRIMER_LEFT_33_SEQUENCE=TAGGACCGATGATTGTGGAA
PRIMER_RIGHT_33_SEQUENCE=TCAGCAGCCCACTTCTTTCT
PRIMER_LEFT_33=826,20
PRIMER_RIGHT_33=1767,20
PRIMER_LEFT_33_TM=58.923
PRIMER_RIGHT_33_TM=60.134
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=942
PRIMER_PAIR_34_PENALTY=1.213123
PRIMER_LEFT_34_PENALTY=0.302821
PRIMER_RIGHT_34_PENALTY=0.910302
PRIMER_LEFT_34_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_34_SEQUENCE=ATGATGCCTGTGAGAAACCA
PRIMER_LEFT_34=343,20
PRIMER_RIGHT_34=1326,20
PRIMER_LEFT_34_TM=59.697
PRIMER_RIGHT_34_TM=59.090
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=984
PRIMER_PAIR_35_PENALTY=1.213123
PRIMER_LEFT_35_PENALTY=0.302821
PRIMER_RIGHT_35_PENALTY=0.910302
PRIMER_LEFT_35_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_35_SEQUENCE=ATGATGCCTGTGAGAAACCA
PRIMER_LEFT_35=342,20
PRIMER_RIGHT_35=1326,20
PRIMER_LEFT_35_TM=59.697
PRIMER_RIGHT_35_TM=59.090
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=985
PRIMER_PAIR_36_PENALTY=1.213123
PRIMER_LEFT_36_PENALTY=0.302821
PRIMER_RIGHT_36_PENALTY=0.910302
PRIMER_LEFT_36_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_36_SEQUENCE=ATGATGCCTGTGAGAAACCA
PRIMER_LEFT_36=341,20
PRIMER_RIGHT_36=1326,20
PRIMER_LEFT_36_TM=59.697
PRIMER_RIGHT_36_TM=59.090
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=986
PRIMER_PAIR_37_PENALTY=1.252520
PRIMER_LEFT_37_PENALTY=1.194956
PRIMER_RIGHT_37_PENALTY=0.057564
PRIMER_LEFT_37_SEQUENCE=GTAGGACCGATGATTGTGGAA
PRIMER_RIGHT_37_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_37=825,21
PRIMER_RIGHT_37=1331,20
PRIMER_LEFT_37_TM=59.805
PRIMER_RIGHT_37_TM=59.942
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=507
PRIMER_PAIR_38_PENALTY=1.290470
PRIMER_LEFT_38_PENALTY=0.109200
PRIMER_RIGHT_38_PENALTY=1.181270
PRIMER_LEFT_38_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_38_SEQUENCE=GACATCCCGAGAACCCTAAAC
PRIMER_LEFT_38=626,20
PRIMER_RIGHT_38=1153,21
PRIMER_LEFT_38_TM=59.891
PRIMER_RIGHT_38_TM=59.819
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=528
PRIMER_PAIR_39_PENALTY=1.309771
PRIMER_LEFT_39_PENALTY=0.291059
PRIMER_RIGHT_39_PENALTY=1.018712
PRIMER_LEFT_39_SEQUENCE=TGGGAATCGTCATATCCTCC
PRIMER_RIGHT_39_SEQUENCE=GACATCCCGAGAACCCTAAA
PRIMER_LEFT_39=324,20
PRIMER_RIGHT_39=1153,20
PRIMER_LEFT_39_TM=59.709
PRIMER_RIGHT_39_TM=58.981
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=6.3000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=830
PRIMER_PAIR_40_PENALTY=1.311275
PRIMER_LEFT_40_PENALTY=1.253711
PRIMER_RIGHT_40_PENALTY=0.057564
PRIMER_LEFT_40_SEQUENCE=CTCGTCAAGGTTGTTCCAAA
PRIMER_RIGHT_40_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_40=346,20
PRIMER_RIGHT_40=1331,20
PRIMER_LEFT_40_TM=58.746
PRIMER_RIGHT_40_TM=59.942
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.8000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=986
PRIMER_PAIR_41_PENALTY=1.316577
PRIMER_LEFT_41_PENALTY=1.076720
PRIMER_RIGHT_41_PENALTY=0.239857
PRIMER_LEFT_41_SEQUENCE=TAGGACCGATGATTGTGGAA
PRIMER_RIGHT_41_SEQUENCE=TGATGCCTGTGAGAAACCAA
PRIMER_LEFT_41=826,20
PRIMER_RIGHT_41=1325,20
PRIMER_LEFT_41_TM=58.923
PRIMER_RIGHT_41_TM=60.240
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=500
PRIMER_PAIR_42_PENALTY=1.321533
PRIMER_LEFT_42_PENALTY=0.302821
PRIMER_RIGHT_42_PENALTY=1.018712
PRIMER_LEFT_42_SEQUENCE=CTCCTCGTCAAGGTTGTTCC
PRIMER_RIGHT_42_SEQUENCE=GACATCCCGAGAACCCTAAA
PRIMER_LEFT_42=343,20
PRIMER_RIGHT_42=1153,20
PRIMER_LEFT_42_TM=59.697
PRIMER_RIGHT_42_TM=58.981
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=811
PRIMER_PAIR_43_PENALTY=1.321533
PRIMER_LEFT_43_PENALTY=0.302821
PRIMER_RIGHT_43_PENALTY=1.018712
PRIMER_LEFT_43_SEQUENCE=CCTCCTCGTCAAGGTTGTTC
PRIMER_RIGHT_43_SEQUENCE=GACATCCCGAGAACCCTAAA
PRIMER_LEFT_43=342,20
PRIMER_RIGHT_43=1153,20
PRIMER_LEFT_43_TM=59.697
PRIMER_RIGHT_43_TM=58.981
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=812
PRIMER_PAIR_44_PENALTY=1.321533
PRIMER_LEFT_44_PENALTY=0.302821
PRIMER_RIGHT_44_PENALTY=1.018712
PRIMER_LEFT_44_SEQUENCE=TCCTCCTCGTCAAGGTTGTT
PRIMER_RIGHT_44_SEQUENCE=GACATCCCGAGAACCCTAAA
PRIMER_LEFT_44=341,20
PRIMER_RIGHT_44=1153,20
PRIMER_LEFT_44_TM=59.697
PRIMER_RIGHT_44_TM=58.981
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.3000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=813
PRIMER_PAIR_45_PENALTY=1.328697
PRIMER_LEFT_45_PENALTY=1.194956
PRIMER_RIGHT_45_PENALTY=0.133741
PRIMER_LEFT_45_SEQUENCE=GTAGGACCGATGATTGTGGAA
PRIMER_RIGHT_45_SEQUENCE=TCAGCAGCCCACTTCTTTCT
PRIMER_LEFT_45=825,21
PRIMER_RIGHT_45=1767,20
PRIMER_LEFT_45_TM=59.805
PRIMER_RIGHT_45_TM=60.134
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=943
PRIMER_PAIR_46_PENALTY=1.336295
PRIMER_LEFT_46_PENALTY=0.884476
PRIMER_RIGHT_46_PENALTY=0.451819
PRIMER_LEFT_46_SEQUENCE=CGTCATATCCTCCTCCTCGT
PRIMER_RIGHT_46_SEQUENCE=TGACATCCCGAGAACCCTAA
PRIMER_LEFT_46=331,20
PRIMER_RIGHT_46=1154,20
PRIMER_LEFT_46_TM=59.116
PRIMER_RIGHT_46_TM=60.452
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.1000
PRIMER_RIGHT_46_END_STABILITY=7.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=824
PRIMER_PAIR_47_PENALTY=1.373809
PRIMER_LEFT_47_PENALTY=0.291059
PRIMER_RIGHT_47_PENALTY=1.082750
PRIMER_LEFT_47_SEQUENCE=TGGGAATCGTCATATCCTCC
PRIMER_RIGHT_47_SEQUENCE=TCTGTGACATCCCGAGAACC
PRIMER_LEFT_47=324,20
PRIMER_RIGHT_47=1158,20
PRIMER_LEFT_47_TM=59.709
PRIMER_RIGHT_47_TM=61.083
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=835
PRIMER_PAIR_48_PENALTY=1.381036
PRIMER_LEFT_48_PENALTY=1.323473
PRIMER_RIGHT_48_PENALTY=0.057564
PRIMER_LEFT_48_SEQUENCE=GATTGTGGAATTACCGTTGGA
PRIMER_RIGHT_48_SEQUENCE=CGAAGATGATGCCTGTGAGA
PRIMER_LEFT_48=836,21
PRIMER_RIGHT_48=1331,20
PRIMER_LEFT_48_TM=59.677
PRIMER_RIGHT_48_TM=59.942
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=496
PRIMER_PAIR_49_PENALTY=1.383464
PRIMER_LEFT_49_PENALTY=0.109200
PRIMER_RIGHT_49_PENALTY=1.274264
PRIMER_LEFT_49_SEQUENCE=CAATGGCGAATTTGATGATG
PRIMER_RIGHT_49_SEQUENCE=ATGCCTGTGAGAAACCAAAGA
PRIMER_LEFT_49=626,20
PRIMER_RIGHT_49=1323,21
PRIMER_LEFT_49_TM=59.891
PRIMER_RIGHT_49_TM=59.726
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=698
=
SEQUENCE_ID=MAGIC.433_4_2221227_2221705_oy-0_wu-0_353475_73477_TRUE
SEQUENCE_TARGET=950,578
SEQUENCE_TEMPLATE=ATCTCTACNAANNNTTTTGGAGGTTCATTAACATCAATGACATTGTTTTACAGTTAAGCGATGGATGTCAATGGAGAAAATACGGACAAAAAATGGCGAAAGGAAACCCGTGCCCTCGAGCTTATTACCGTTGCACAATGGCTGTTGGATGNCCNGTTCGCAAGCAAGTACGTTNCCTAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGATGACCTAACAGGTGCAACGTTGCGCGGANGANAGAACCATTCTCATAACAACCTACGAAGGAAACCATAACCATCCATTACCNCCNGCGGCTATGAACATGGCTTCAACTACAANNGCAGCCGCAAGCATGCTTCTCTCAGGCTCCACCATGTCGAACCAAGACGNTTTAATGAACCCAACAAATCTCTTGGCTCGAACCATATTACCGTGTTCCTCAAGCATGGCTACTATCTCAGCCTCTGCACCATTCCCAACCATTACATTAGACCTCACANAGTCACCCAACGGGAACAATCCAACCAATAACCCGCTGATGCAATTCTCTCAACGGTCTGGTTTGGTGGAGTTGAACCAATCGGTTTTGCCTCATATGATGGGTCAGGCTTTGTACTACAACCAACAGTCTAAGTTTTCGGGTTTACATATGCCGTCTCAGCCGCTAAACNCTGGTGAGAGTGTTAGCGCCGCTACTGCCGCAATCGCCTCCAATCCCAACTTTGCNGCGGCTCTAGCTGCAGCNATAACTTCNATTATCAACGGTTCGAANAATCAGCAGAATGGGAACAANAATAACAGTAATGTTACAACGAGCAACGTTGACAATAGGCAATAACANTNTTTTATAAGTTTTAGTTAGGGACTTTTTATCGGTCGATTTTGTTTTNNTNTNNNNNNNNNNNNNNNNNNTTTAGTTACGGCNTTTTTTTGTTTNTTCTTTTCTTTCCCNAACAACAAGTATTGAGAGCANTNNNNNNNNNNNNNNNNNNNNNNNNNCTGTATNATCNACAATTGTGNTCAGAAATATNAAATTNNTTAANTTTTCCANAAGCTATTAATTCTTATGAAGTCATCAAATGCGNATCTATGTGCCNNNNATTATGANCAAACTCGATNTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGTGTAATATTTTATTNNNNNNNNNNNAATANNNTTTNCTTTAGGGAGCCCAANCACTTAGACTCTGAACATCGGTACGATGTTTTATTTGTGGTGGATATATGGAACTGCTATTAACGTACTTACTCACTCTAAAATTAGTCGATGCTGACCTCAAGAGTTTGGCTTGTGAAAAGTTGATGACCAGACTAAATCGATATGTCANTTGTCAAGTGTGGCATTATTTTAAAGGNTGNTTANNNNTATAATTTAAGTGAANGATTGGACATTTNATCNAANAAAAANCAGNATTNGACTGGATNTTGANAAAGTGAAGAATGTTNCNNCATANNCNANTNNAANCATAGTTGAGAAACTANGANATNTACATTTGTATTATAAANTAAATTATANTATNNCTTTNAANNNNNNNNNNNNNNNNNNNNNNNACAATGCGNATATCAATATTTTANTAANAAAAATGTCGGANTNNAGTATNNNNNNNNNNNNNNATATGTTGGTATNAAGTTTATATATGATNATATNGANTTTTNGGAGANNNNNNNNNNNNNNNNNNNNNNNNNCNGTTAATTTATANTNGTATNNAATTTTATGCTAAGTNTGTANTANNANNNNNNNNNNNNNNNNGNANTANATANTTATCCNTAATAGTNAANGGTAAATTAANNNNNNNTNTTNNNANATTTGGACATTTAACTNTAAAAANTNTGAAAANANCACTTTTTNTTAGNGTAACGGAGATGGACATATTCTGCTATAANAAATAACGGTTNTNCTCTCNATCATCTTCTTCTTTNNTTCTTNTTTGTTTTTGATGAGTGAAATCGAAAAGATAGTATAGGGATTCCAAATGGATCNCAATTAAATTTGTATTGATGAGTAAAATCTTAACCCTAATGAGAGTATAGGGAACTCAAATGCTTCAATCATAGACANATATCGAATCAATTATGAATTTTAATTAAAAATCTAAATTNTTTGAGAACTTTGGATAGNGAGTTACTTGGATAAACTTNATCCATGGAGTTCTTTGAATCCCTTANAGATGACAACATCTTATCAATAGAGACGGCCTTTTCATTCTCACACAGACTCTTATCTCTNGTANNNNNNAATTTGGGATTATTTTAAAAATTACANTTAAAAGCTGATCCTAATTTCATAAGAACCATAATTGCAAAATCAAATTNGAATCNAAATACAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_12763232_12763566_wu-0_po-0_17827_30669_TRUE
SEQUENCE_TARGET=950,434
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_14295471_14295645_ct-1_rsch-4_25041_34094_TRUE
SEQUENCE_TARGET=950,274
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_2151093_2151291_ws-0_wil-2_28003_306818_TRUE
SEQUENCE_TARGET=950,298
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCAAATCTCCGCTTCACTCTCTCTTACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_14602033_14602244_sf-2_oy-0_100524_456448_TRUE
SEQUENCE_TARGET=950,311
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_6147432_6147442_bur-0_hi-0_99_317921_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=CTTCATGTGTTTTCTAAGCTACNCNNNNNNNNNNATGCTATAAAGTATCAACCTTTAATTAGAAAACTAGTTTAAAGTNCCAAAGGTTATGTTTTCAGAACTGCCCAGAAACATATATATGTACTTGTTAGAACTTGATTTNNTTTTTTNATTGGTGATGTGAGGATTTAGTCNACAGGATCGTTCTTCCATGACAGAAGCATCACACTTGGAACGCTTATGGGATTTAGTTTCACNGCAACTATGCCGATGCCATTNAGAGCNTCTTCACGCCGCCACGTGTCACCTTCCGTCGCAATCTCACGAGCTTCTAGCTCCAATGCAAGACGCAACCACCAGAGAAAGCGTCCTCCTTCAAATTCGGCTGAGCCTGAGCCGCATCGCCGCCGTAAATGGTGGCGGTTTTGCCGAGATGACGATGACGACGCCGCCGGAAATGGAATCCACCGTGGAACAGGAGATTCAAAGCGGTCATCGCTNGGGGAGTATNTNGAGGTGGAACGGAGGTTTGGAGATGAAGCCGTTNACAACTCCGCCGAGGCAGAGCTAGAGGATGCGGTGGTGGCACGGTACCAAGATCAGCAGCCGGTGATGGGGGAGCGGGCATTGTTCGCTGACGGAAGGGTTCTTCCTCCGGCTTCAGNTGAAGNGGTTACCGGAGAANGNACGCCGGTNGCCACGNCCCTTTGTAGATTTCCGGNNTCTTTAACCNGTATATGCAGCGGTGGNGGAGGNTGAATACGTCGGTTCATGTGTGNGNNNNNNATTTGTATTTAGACTTAAATTTTTGTATNNNNNNNNNNNNNNNNGAATCATTGTTTGTGGAAAATATAACATGTTGNATGTAGTCATTGTCAAANTAATTATTTGAGANNNAANNNNNNNTNNNAAGGAGAATTANNAANNANANTANNNANANNNGNAATANTNNNNNNTNNNCNTNNANNANNNNANACNTGNANANCTNTNGNTNNNAANTGGAGNNNNNCNCTNNATNANNNTNTTNATTTNAAATGNTAANNGTTGNTCTATCANATTGAGTTTTTGCTTCAAANNNNNNNNNNNNNNNNTCTTTATACTAGTCAGTCTTTGGACAATAATTTGACCAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANGAGTGTGCCACATTNGTGGTTANTTTTCCACTAGTATTTCTGAAAGTAAAACAATTAACCACAAATCATCCTCATAACATAAGAGGAGAAGATTCCTAACGACGAAGCAAAATTATCGAACAAGTGGACACACACAAGAAACCGAAAATACGGCAAAATGTTTGTTATGTGTATCCATATTTTATANCAATTGAATTAATAAAATAAACTGTANAAACAAGAAACAATTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAANCCTCGAGTGNTGTTAAAAAAAGGAAGAAGGAAAAAGAATAAAAACTCAAAGGGAACGCAACACACTNAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTNNNCAGCAGCAGCAGNAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGCTGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCACCGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCTGAATGATTTTTTCCTTGGGTAGAGTCAAAATTTTGTTTGACAAATGTTGGAATCTGCGTTGACTCGACTCGTAATCTTCTCCGGCGCACTACATGTCTGATTCTGAGCTTTCGAAAGTATGGTTGATTGATTTTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.106709
PRIMER_LEFT_0_PENALTY=0.049859
PRIMER_RIGHT_0_PENALTY=0.056850
PRIMER_LEFT_0_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_0_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_0=498,20
PRIMER_RIGHT_0=1303,20
PRIMER_LEFT_0_TM=60.050
PRIMER_RIGHT_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=806
PRIMER_PAIR_1_PENALTY=0.243451
PRIMER_LEFT_1_PENALTY=0.186600
PRIMER_RIGHT_1_PENALTY=0.056850
PRIMER_LEFT_1_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_1_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_1=451,20
PRIMER_RIGHT_1=1303,20
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=853
PRIMER_PAIR_2_PENALTY=0.311463
PRIMER_LEFT_2_PENALTY=0.254613
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_2_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_2=536,20
PRIMER_RIGHT_2=1303,20
PRIMER_LEFT_2_TM=60.255
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=768
PRIMER_PAIR_3_PENALTY=0.410901
PRIMER_LEFT_3_PENALTY=0.254613
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_LEFT_3_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_3_SEQUENCE=GTGTTGCGTTCCCTTTGAGT
PRIMER_LEFT_3=536,20
PRIMER_RIGHT_3=1505,20
PRIMER_LEFT_3_TM=60.255
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=970
PRIMER_PAIR_4_PENALTY=0.422170
PRIMER_LEFT_4_PENALTY=0.365320
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_4_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_4=456,20
PRIMER_RIGHT_4=1303,20
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=848
PRIMER_PAIR_5_PENALTY=0.463402
PRIMER_LEFT_5_PENALTY=0.049859
PRIMER_RIGHT_5_PENALTY=0.413543
PRIMER_LEFT_5_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_5_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_5=498,20
PRIMER_RIGHT_5=1300,20
PRIMER_LEFT_5_TM=60.050
PRIMER_RIGHT_5_TM=59.586
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=803
PRIMER_PAIR_6_PENALTY=0.463402
PRIMER_LEFT_6_PENALTY=0.049859
PRIMER_RIGHT_6_PENALTY=0.413543
PRIMER_LEFT_6_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_6_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_6=498,20
PRIMER_RIGHT_6=1301,20
PRIMER_LEFT_6_TM=60.050
PRIMER_RIGHT_6_TM=59.586
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=804
PRIMER_PAIR_7_PENALTY=0.463402
PRIMER_LEFT_7_PENALTY=0.049859
PRIMER_RIGHT_7_PENALTY=0.413543
PRIMER_LEFT_7_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_7_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_7=498,20
PRIMER_RIGHT_7=1302,20
PRIMER_LEFT_7_TM=60.050
PRIMER_RIGHT_7_TM=59.586
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=805
PRIMER_PAIR_8_PENALTY=0.478724
PRIMER_LEFT_8_PENALTY=0.049859
PRIMER_RIGHT_8_PENALTY=0.428866
PRIMER_LEFT_8_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_8_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_8=498,20
PRIMER_RIGHT_8=1291,20
PRIMER_LEFT_8_TM=60.050
PRIMER_RIGHT_8_TM=59.571
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=794
PRIMER_PAIR_9_PENALTY=0.530362
PRIMER_LEFT_9_PENALTY=0.254613
PRIMER_RIGHT_9_PENALTY=0.275749
PRIMER_LEFT_9_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_9_SEQUENCE=TGCGTTCCCTTTGAGTTTTT
PRIMER_LEFT_9=536,20
PRIMER_RIGHT_9=1501,20
PRIMER_LEFT_9_TM=60.255
PRIMER_RIGHT_9_TM=59.724
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=966
PRIMER_PAIR_10_PENALTY=0.530362
PRIMER_LEFT_10_PENALTY=0.254613
PRIMER_RIGHT_10_PENALTY=0.275749
PRIMER_LEFT_10_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_10_SEQUENCE=TTGCGTTCCCTTTGAGTTTT
PRIMER_LEFT_10=536,20
PRIMER_RIGHT_10=1502,20
PRIMER_LEFT_10_TM=60.255
PRIMER_RIGHT_10_TM=59.724
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=967
PRIMER_PAIR_11_PENALTY=0.530545
PRIMER_LEFT_11_PENALTY=0.473695
PRIMER_RIGHT_11_PENALTY=0.056850
PRIMER_LEFT_11_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_11_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_11=499,20
PRIMER_RIGHT_11=1303,20
PRIMER_LEFT_11_TM=59.526
PRIMER_RIGHT_11_TM=59.943
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=805
PRIMER_PAIR_12_PENALTY=0.567181
PRIMER_LEFT_12_PENALTY=0.510331
PRIMER_RIGHT_12_PENALTY=0.056850
PRIMER_LEFT_12_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_12_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_12=445,20
PRIMER_RIGHT_12=1303,20
PRIMER_LEFT_12_TM=60.510
PRIMER_RIGHT_12_TM=59.943
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=859
PRIMER_PAIR_13_PENALTY=0.600143
PRIMER_LEFT_13_PENALTY=0.186600
PRIMER_RIGHT_13_PENALTY=0.413543
PRIMER_LEFT_13_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_13_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_13=451,20
PRIMER_RIGHT_13=1300,20
PRIMER_LEFT_13_TM=59.813
PRIMER_RIGHT_13_TM=59.586
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=850
PRIMER_PAIR_14_PENALTY=0.600143
PRIMER_LEFT_14_PENALTY=0.186600
PRIMER_RIGHT_14_PENALTY=0.413543
PRIMER_LEFT_14_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_14_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_14=451,20
PRIMER_RIGHT_14=1301,20
PRIMER_LEFT_14_TM=59.813
PRIMER_RIGHT_14_TM=59.586
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=851
PRIMER_PAIR_15_PENALTY=0.600143
PRIMER_LEFT_15_PENALTY=0.186600
PRIMER_RIGHT_15_PENALTY=0.413543
PRIMER_LEFT_15_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_15_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_15=451,20
PRIMER_RIGHT_15=1302,20
PRIMER_LEFT_15_TM=59.813
PRIMER_RIGHT_15_TM=59.586
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=852
PRIMER_PAIR_16_PENALTY=0.615466
PRIMER_LEFT_16_PENALTY=0.186600
PRIMER_RIGHT_16_PENALTY=0.428866
PRIMER_LEFT_16_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_16_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_16=451,20
PRIMER_RIGHT_16=1291,20
PRIMER_LEFT_16_TM=59.813
PRIMER_RIGHT_16_TM=59.571
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=11.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=841
PRIMER_PAIR_17_PENALTY=0.635218
PRIMER_LEFT_17_PENALTY=0.578368
PRIMER_RIGHT_17_PENALTY=0.056850
PRIMER_LEFT_17_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_17_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_17=346,20
PRIMER_RIGHT_17=1303,20
PRIMER_LEFT_17_TM=60.578
PRIMER_RIGHT_17_TM=59.943
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=11.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=958
PRIMER_PAIR_18_PENALTY=0.668156
PRIMER_LEFT_18_PENALTY=0.254613
PRIMER_RIGHT_18_PENALTY=0.413543
PRIMER_LEFT_18_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_18_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_18=536,20
PRIMER_RIGHT_18=1300,20
PRIMER_LEFT_18_TM=60.255
PRIMER_RIGHT_18_TM=59.586
PRIMER_LEFT_18_GC_PERCENT=60.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=765
PRIMER_PAIR_19_PENALTY=0.668156
PRIMER_LEFT_19_PENALTY=0.254613
PRIMER_RIGHT_19_PENALTY=0.413543
PRIMER_LEFT_19_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_19_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_19=536,20
PRIMER_RIGHT_19=1301,20
PRIMER_LEFT_19_TM=60.255
PRIMER_RIGHT_19_TM=59.586
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=766
PRIMER_PAIR_20_PENALTY=0.668156
PRIMER_LEFT_20_PENALTY=0.254613
PRIMER_RIGHT_20_PENALTY=0.413543
PRIMER_LEFT_20_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_20_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_20=536,20
PRIMER_RIGHT_20=1302,20
PRIMER_LEFT_20_TM=60.255
PRIMER_RIGHT_20_TM=59.586
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=767
PRIMER_PAIR_21_PENALTY=0.683479
PRIMER_LEFT_21_PENALTY=0.254613
PRIMER_RIGHT_21_PENALTY=0.428866
PRIMER_LEFT_21_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_21_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_21=536,20
PRIMER_RIGHT_21=1291,20
PRIMER_LEFT_21_TM=60.255
PRIMER_RIGHT_21_TM=59.571
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=756
PRIMER_PAIR_22_PENALTY=0.768641
PRIMER_LEFT_22_PENALTY=0.711791
PRIMER_RIGHT_22_PENALTY=0.056850
PRIMER_LEFT_22_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_22_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_22=453,20
PRIMER_RIGHT_22=1303,20
PRIMER_LEFT_22_TM=59.288
PRIMER_RIGHT_22_TM=59.943
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=851
PRIMER_PAIR_23_PENALTY=0.778863
PRIMER_LEFT_23_PENALTY=0.365320
PRIMER_RIGHT_23_PENALTY=0.413543
PRIMER_LEFT_23_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_23_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_23=456,20
PRIMER_RIGHT_23=1300,20
PRIMER_LEFT_23_TM=59.635
PRIMER_RIGHT_23_TM=59.586
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=845
PRIMER_PAIR_24_PENALTY=0.778863
PRIMER_LEFT_24_PENALTY=0.365320
PRIMER_RIGHT_24_PENALTY=0.413543
PRIMER_LEFT_24_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_24_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_24=456,20
PRIMER_RIGHT_24=1301,20
PRIMER_LEFT_24_TM=59.635
PRIMER_RIGHT_24_TM=59.586
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=846
PRIMER_PAIR_25_PENALTY=0.778863
PRIMER_LEFT_25_PENALTY=0.365320
PRIMER_RIGHT_25_PENALTY=0.413543
PRIMER_LEFT_25_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_25_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_25=456,20
PRIMER_RIGHT_25=1302,20
PRIMER_LEFT_25_TM=59.635
PRIMER_RIGHT_25_TM=59.586
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=847
PRIMER_PAIR_26_PENALTY=0.794186
PRIMER_LEFT_26_PENALTY=0.365320
PRIMER_RIGHT_26_PENALTY=0.428866
PRIMER_LEFT_26_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_26_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_26=456,20
PRIMER_RIGHT_26=1291,20
PRIMER_LEFT_26_TM=59.635
PRIMER_RIGHT_26_TM=59.571
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=836
PRIMER_PAIR_27_PENALTY=0.887238
PRIMER_LEFT_27_PENALTY=0.473695
PRIMER_RIGHT_27_PENALTY=0.413543
PRIMER_LEFT_27_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_27_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_27=499,20
PRIMER_RIGHT_27=1300,20
PRIMER_LEFT_27_TM=59.526
PRIMER_RIGHT_27_TM=59.586
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=802
PRIMER_PAIR_28_PENALTY=0.887238
PRIMER_LEFT_28_PENALTY=0.473695
PRIMER_RIGHT_28_PENALTY=0.413543
PRIMER_LEFT_28_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_28_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_28=499,20
PRIMER_RIGHT_28=1301,20
PRIMER_LEFT_28_TM=59.526
PRIMER_RIGHT_28_TM=59.586
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=803
PRIMER_PAIR_29_PENALTY=0.887238
PRIMER_LEFT_29_PENALTY=0.473695
PRIMER_RIGHT_29_PENALTY=0.413543
PRIMER_LEFT_29_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_29_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_29=499,20
PRIMER_RIGHT_29=1302,20
PRIMER_LEFT_29_TM=59.526
PRIMER_RIGHT_29_TM=59.586
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=804
PRIMER_PAIR_30_PENALTY=0.889843
PRIMER_LEFT_30_PENALTY=0.832993
PRIMER_RIGHT_30_PENALTY=0.056850
PRIMER_LEFT_30_SEQUENCE=CAACCACCAGAGAAAGCGTC
PRIMER_RIGHT_30_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_30=329,20
PRIMER_RIGHT_30=1303,20
PRIMER_LEFT_30_TM=60.833
PRIMER_RIGHT_30_TM=59.943
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=975
PRIMER_PAIR_31_PENALTY=0.889843
PRIMER_LEFT_31_PENALTY=0.832993
PRIMER_RIGHT_31_PENALTY=0.056850
PRIMER_LEFT_31_SEQUENCE=GACGCAACCACCAGAGAAAG
PRIMER_RIGHT_31_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_31=325,20
PRIMER_RIGHT_31=1303,20
PRIMER_LEFT_31_TM=60.833
PRIMER_RIGHT_31_TM=59.943
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=0.889843
PRIMER_LEFT_32_PENALTY=0.832993
PRIMER_RIGHT_32_PENALTY=0.056850
PRIMER_LEFT_32_SEQUENCE=AAGACGCAACCACCAGAGAA
PRIMER_RIGHT_32_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_32=323,20
PRIMER_RIGHT_32=1303,20
PRIMER_LEFT_32_TM=60.833
PRIMER_RIGHT_32_TM=59.943
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=981
PRIMER_PAIR_33_PENALTY=0.902560
PRIMER_LEFT_33_PENALTY=0.473695
PRIMER_RIGHT_33_PENALTY=0.428866
PRIMER_LEFT_33_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_33_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_33=499,20
PRIMER_RIGHT_33=1291,20
PRIMER_LEFT_33_TM=59.526
PRIMER_RIGHT_33_TM=59.571
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=793
PRIMER_PAIR_34_PENALTY=0.919829
PRIMER_LEFT_34_PENALTY=0.862979
PRIMER_RIGHT_34_PENALTY=0.056850
PRIMER_LEFT_34_SEQUENCE=ACGGAGGTTTGGAGATGAAG
PRIMER_RIGHT_34_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_34=500,20
PRIMER_RIGHT_34=1303,20
PRIMER_LEFT_34_TM=59.137
PRIMER_RIGHT_34_TM=59.943
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=804
PRIMER_PAIR_35_PENALTY=0.922928
PRIMER_LEFT_35_PENALTY=0.254613
PRIMER_RIGHT_35_PENALTY=0.668315
PRIMER_LEFT_35_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_35_SEQUENCE=TGTTGCGTTCCCTTTGAGTT
PRIMER_LEFT_35=536,20
PRIMER_RIGHT_35=1504,20
PRIMER_LEFT_35_TM=60.255
PRIMER_RIGHT_35_TM=60.668
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=969
PRIMER_PAIR_36_PENALTY=0.923874
PRIMER_LEFT_36_PENALTY=0.510331
PRIMER_RIGHT_36_PENALTY=0.413543
PRIMER_LEFT_36_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_36_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_36=445,20
PRIMER_RIGHT_36=1300,20
PRIMER_LEFT_36_TM=60.510
PRIMER_RIGHT_36_TM=59.586
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=856
PRIMER_PAIR_37_PENALTY=0.923874
PRIMER_LEFT_37_PENALTY=0.510331
PRIMER_RIGHT_37_PENALTY=0.413543
PRIMER_LEFT_37_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_37_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_37=445,20
PRIMER_RIGHT_37=1301,20
PRIMER_LEFT_37_TM=60.510
PRIMER_RIGHT_37_TM=59.586
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=857
PRIMER_PAIR_38_PENALTY=0.923874
PRIMER_LEFT_38_PENALTY=0.510331
PRIMER_RIGHT_38_PENALTY=0.413543
PRIMER_LEFT_38_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_38_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_38=445,20
PRIMER_RIGHT_38=1302,20
PRIMER_LEFT_38_TM=60.510
PRIMER_RIGHT_38_TM=59.586
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=858
PRIMER_PAIR_39_PENALTY=0.939197
PRIMER_LEFT_39_PENALTY=0.510331
PRIMER_RIGHT_39_PENALTY=0.428866
PRIMER_LEFT_39_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_39_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_39=445,20
PRIMER_RIGHT_39=1291,20
PRIMER_LEFT_39_TM=60.510
PRIMER_RIGHT_39_TM=59.571
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=847
PRIMER_PAIR_40_PENALTY=0.991911
PRIMER_LEFT_40_PENALTY=0.578368
PRIMER_RIGHT_40_PENALTY=0.413543
PRIMER_LEFT_40_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_40_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_40=346,20
PRIMER_RIGHT_40=1300,20
PRIMER_LEFT_40_TM=60.578
PRIMER_RIGHT_40_TM=59.586
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=955
PRIMER_PAIR_41_PENALTY=0.991911
PRIMER_LEFT_41_PENALTY=0.578368
PRIMER_RIGHT_41_PENALTY=0.413543
PRIMER_LEFT_41_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_41_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_41=346,20
PRIMER_RIGHT_41=1301,20
PRIMER_LEFT_41_TM=60.578
PRIMER_RIGHT_41_TM=59.586
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=11.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=956
PRIMER_PAIR_42_PENALTY=0.991911
PRIMER_LEFT_42_PENALTY=0.578368
PRIMER_RIGHT_42_PENALTY=0.413543
PRIMER_LEFT_42_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_42_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_42=346,20
PRIMER_RIGHT_42=1302,20
PRIMER_LEFT_42_TM=60.578
PRIMER_RIGHT_42_TM=59.586
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=11.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=957
PRIMER_PAIR_43_PENALTY=1.007234
PRIMER_LEFT_43_PENALTY=0.578368
PRIMER_RIGHT_43_PENALTY=0.428866
PRIMER_LEFT_43_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_43_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_43=346,20
PRIMER_RIGHT_43=1291,20
PRIMER_LEFT_43_TM=60.578
PRIMER_RIGHT_43_TM=59.571
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=946
PRIMER_PAIR_44_PENALTY=1.036122
PRIMER_LEFT_44_PENALTY=0.254613
PRIMER_RIGHT_44_PENALTY=0.781508
PRIMER_LEFT_44_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_44_SEQUENCE=GTTGCGTTCCCTTTGAGTTT
PRIMER_LEFT_44=536,20
PRIMER_RIGHT_44=1503,20
PRIMER_LEFT_44_TM=60.255
PRIMER_RIGHT_44_TM=59.218
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=968
PRIMER_PAIR_45_PENALTY=1.125334
PRIMER_LEFT_45_PENALTY=0.711791
PRIMER_RIGHT_45_PENALTY=0.413543
PRIMER_LEFT_45_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_45_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_45=453,20
PRIMER_RIGHT_45=1300,20
PRIMER_LEFT_45_TM=59.288
PRIMER_RIGHT_45_TM=59.586
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=848
PRIMER_PAIR_46_PENALTY=1.125334
PRIMER_LEFT_46_PENALTY=0.711791
PRIMER_RIGHT_46_PENALTY=0.413543
PRIMER_LEFT_46_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_46_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_46=453,20
PRIMER_RIGHT_46=1301,20
PRIMER_LEFT_46_TM=59.288
PRIMER_RIGHT_46_TM=59.586
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=849
PRIMER_PAIR_47_PENALTY=1.125334
PRIMER_LEFT_47_PENALTY=0.711791
PRIMER_RIGHT_47_PENALTY=0.413543
PRIMER_LEFT_47_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_47_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_47=453,20
PRIMER_RIGHT_47=1302,20
PRIMER_LEFT_47_TM=59.288
PRIMER_RIGHT_47_TM=59.586
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=850
PRIMER_PAIR_48_PENALTY=1.140657
PRIMER_LEFT_48_PENALTY=0.711791
PRIMER_RIGHT_48_PENALTY=0.428866
PRIMER_LEFT_48_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_48_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_48=453,20
PRIMER_RIGHT_48=1291,20
PRIMER_LEFT_48_TM=59.288
PRIMER_RIGHT_48_TM=59.571
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=839
PRIMER_PAIR_49_PENALTY=1.152585
PRIMER_LEFT_49_PENALTY=1.095734
PRIMER_RIGHT_49_PENALTY=0.056850
PRIMER_LEFT_49_SEQUENCE=CAGAGCTAGAGGATGCGGTG
PRIMER_RIGHT_49_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_49=541,20
PRIMER_RIGHT_49=1303,20
PRIMER_LEFT_49_TM=61.096
PRIMER_RIGHT_49_TM=59.943
PRIMER_LEFT_49_GC_PERCENT=60.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=763
=
SEQUENCE_ID=MAGIC.287_2_9254784_9255143_can-0_tsu-0_1340_3036373_TRUE
SEQUENCE_TARGET=950,459
SEQUENCE_TEMPLATE=CNTNTNNNTANTNNTTGNTCATNTNANANGNNNCNNTGNNCATTNANNNNNGCNNNCNATTTNGNTANNNNNTTTGNNNNTTGTTNTNNNNGNGANANAGTNAGAAAAAGATTTAGGCAAGATTCGTNTTTTACATTGATCATACGAGATCAAAAAGGAAAANCACCAAAACCTTGATGAAGAAGTAAAACAGATGANGTTAGCTAAACCGAGAAAGTAAAACTATCTTAAACCTTGATGAAGAAGGAAAAACACCAAAACCTTAATNTTAANNNNNAAAAANAAAATCACCANAATAACTTNNATNTAAAAANNNNNAAAAGCAACAAACCCCATACTCNATATCATTCAGNCACAAAATATTTGTATAAGCGAAAATTGAAGATNGATNAAAGTTAAAGGNNNNNNNNNNNNNNNNGAAGAATGTACATACAATAANANAANGGTTACCTTTTACANTATNAAAAAGGTNAAGGGGTCAAATNTGAAGNAACAGTTAAATTAAGGTCCAANTATTNTTANTTNAAACGCTGCGTTTAGTAACTTGCTCTCCAAGGAGATCTAGCTTGTTCTACTCGCACGTGATTATAACCCNTNCATAACTGTNTATANANATAGACGTCGTCCTTGTGCTTGCAGAGAAAGATAAAAACAGAAAAAGANAAGGAACTTGCAGAGAAAGCAAAAAAGAAAGAAGAAGCGAAGAAGAGAAACTAAGATTCAGGTAACTGCAAGCTTTGAATCTCTTCNTTAATTTCTTGCTTATGAATTNCTTATGATCGATAATGAGNCTGGNGAGTTCTGTTTCACTGAATCCGCAGCAAATTTTGAGNAATTCGTTAGTTTTTGTTTGAATCTCTCTTAGGAATTTGGTCNAACACTTGGTGGNNNNNNNNNNNNTTNNTNANTCGNTTTNGNANTGNTGNTTNTTGNTTNNATTNNNNNNNNNNNGNNNNNNNNNNNNNNNGANNNNTGNNNTNANTTNNTNGNNGNNNNTGANNTNNTTATTTTGGTTGNTNAGAATCCGAATGNNNNNNNNNNNNNGTTAGGTGAATTTGTTTATGTTTGTGAGACTTTAGGGTTCTTGAGACTTGANTTTGATTATTNGATTAACATAACTGGTTTTGATAGATGTGAGGATTGCTTTGTGTAATATCTTAATTGGATCATTCGTATTCANGAGATTTCTTTCTGTTTTCAGATGGCTTCCCAGACAAATCAAACCGTGGTGGATACCNAGAAAATCGAAACCGAGAACCCGCCAAAACCGCAAGTGCCTGCATCATCATGCAGGAAGAGGGTGAAGGATGATAACGCCACTTTCTTTGCAAACNTCAAGGATCACATGGATGAATTCATNCATGCCTCAATGGATGAACACAAGACTTGTTTCAAAAACACCATGGACAAGGNTNTTTCTCAATCNTCTATATCNCTTGATNACATNGTGGATTTNGCATGCTTATGTGTTTGATNNATGTTGAAGATGCTTAGAATGCATAATTATCTCTNTCTNGTTATTTAGTGTTAATCTATCTCTTAACTTATTTGGTTGGATCTCTCAGATATTCGGGAGCTTCTCCAAGGCGGAGGCAGTTGCAGAGAAGCAAATTGAGGCTAAGGAAGTGGTCGAGATTCACTCTCCTCTTCAGACAGCAGTGACTAAGTAGAGATTTTCACAACTTGTGAGCTCAGAAATAATCATGGAGTGTGTTTTCTTTNTTNANGTATTTGGGTGTTAATACTCTTAGAACCTGCTTCTATCTCTAACCCATTCTAAATCAGACAATTTGTTTCTAGANCATTGCGTTTAATCNTCTATACTAATNTCATTTNNNNNNNAAGTNTTTATCATNCCCTACTTNNNNNNTATTATTACTTTGCTATGAAAATATGNCNATNGACCGCCAAACTTAANTAAAGTTACTGNNTTTTTTTGTGGGTTTGGCTTGAANCTCCGTTGGACTGNAATTAATAAANNNNNTGATCCTNGAGGAAAGAAATATATCTTAGTTTCAATGTTATAGTGCNGTGTTTGTGAAACGANTNACCTTCTGTGAATTNTTTTTTTGATAAAATTATAGNTTTACTTGTCCCTCAAGAAGAATGTNNATACAATTGTTGTCATAAANTTTNNNCCCTTAAAACATACTATNTTTAACTTNTCCCTCAAGAATAATTCTTCTTTAGTCTAAAGGTGTGTTATTGAATCATAGATATTTGTTCNTTCNTTTTGCCNAAAAAGAAAAGGATATTTGTTCATTCTCTANATTTAAAATCTACAATTGAATAAAGAAACAAATTAANAAAAANACAATTACCAANCTCTGTTAATTAAGNTTTAACATNAATCGATGCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.947276
PRIMER_LEFT_0_PENALTY=1.376776
PRIMER_RIGHT_0_PENALTY=0.570500
PRIMER_LEFT_0_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_0_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_0=665,21
PRIMER_RIGHT_0=1632,20
PRIMER_LEFT_0_TM=59.623
PRIMER_RIGHT_0_TM=59.430
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=968
PRIMER_PAIR_1_PENALTY=1.947276
PRIMER_LEFT_1_PENALTY=1.376776
PRIMER_RIGHT_1_PENALTY=0.570500
PRIMER_LEFT_1_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_1_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_1=665,21
PRIMER_RIGHT_1=1634,20
PRIMER_LEFT_1_TM=59.623
PRIMER_RIGHT_1_TM=59.430
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=970
PRIMER_PAIR_2_PENALTY=2.318294
PRIMER_LEFT_2_PENALTY=1.747794
PRIMER_RIGHT_2_PENALTY=0.570500
PRIMER_LEFT_2_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_2_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_2=664,21
PRIMER_RIGHT_2=1632,20
PRIMER_LEFT_2_TM=59.252
PRIMER_RIGHT_2_TM=59.430
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=969
PRIMER_PAIR_3_PENALTY=2.318294
PRIMER_LEFT_3_PENALTY=1.747794
PRIMER_RIGHT_3_PENALTY=0.570500
PRIMER_LEFT_3_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_3_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_3=664,21
PRIMER_RIGHT_3=1634,20
PRIMER_LEFT_3_TM=59.252
PRIMER_RIGHT_3_TM=59.430
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=971
PRIMER_PAIR_4_PENALTY=2.318294
PRIMER_LEFT_4_PENALTY=1.747794
PRIMER_RIGHT_4_PENALTY=0.570500
PRIMER_LEFT_4_SEQUENCE=AAGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_4_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_4=663,21
PRIMER_RIGHT_4=1632,20
PRIMER_LEFT_4_TM=59.252
PRIMER_RIGHT_4_TM=59.430
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=970
PRIMER_PAIR_5_PENALTY=2.318294
PRIMER_LEFT_5_PENALTY=1.747794
PRIMER_RIGHT_5_PENALTY=0.570500
PRIMER_LEFT_5_SEQUENCE=AAGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_5_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_5=663,21
PRIMER_RIGHT_5=1634,20
PRIMER_LEFT_5_TM=59.252
PRIMER_RIGHT_5_TM=59.430
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.7000
PRIMER_PAIR_5_COMPL_ANY=8.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=972
PRIMER_PAIR_6_PENALTY=2.342097
PRIMER_LEFT_6_PENALTY=1.771597
PRIMER_RIGHT_6_PENALTY=0.570500
PRIMER_LEFT_6_SEQUENCE=GGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_6_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_6=665,20
PRIMER_RIGHT_6=1632,20
PRIMER_LEFT_6_TM=58.228
PRIMER_RIGHT_6_TM=59.430
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=968
PRIMER_PAIR_7_PENALTY=2.342097
PRIMER_LEFT_7_PENALTY=1.771597
PRIMER_RIGHT_7_PENALTY=0.570500
PRIMER_LEFT_7_SEQUENCE=GGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_7_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_7=665,20
PRIMER_RIGHT_7=1634,20
PRIMER_LEFT_7_TM=58.228
PRIMER_RIGHT_7_TM=59.430
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.7000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=970
PRIMER_PAIR_8_PENALTY=2.650021
PRIMER_LEFT_8_PENALTY=1.376776
PRIMER_RIGHT_8_PENALTY=1.273245
PRIMER_LEFT_8_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_8_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_8=665,21
PRIMER_RIGHT_8=1635,21
PRIMER_LEFT_8_TM=59.623
PRIMER_RIGHT_8_TM=59.727
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.8000
PRIMER_RIGHT_8_END_STABILITY=8.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=2.744683
PRIMER_LEFT_9_PENALTY=2.174183
PRIMER_RIGHT_9_PENALTY=0.570500
PRIMER_LEFT_9_SEQUENCE=AGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_9_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_9=664,20
PRIMER_RIGHT_9=1632,20
PRIMER_LEFT_9_TM=57.826
PRIMER_RIGHT_9_TM=59.430
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=969
PRIMER_PAIR_10_PENALTY=2.744683
PRIMER_LEFT_10_PENALTY=2.174183
PRIMER_RIGHT_10_PENALTY=0.570500
PRIMER_LEFT_10_SEQUENCE=AGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_10_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_10=664,20
PRIMER_RIGHT_10=1634,20
PRIMER_LEFT_10_TM=57.826
PRIMER_RIGHT_10_TM=59.430
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.7000
PRIMER_PAIR_10_COMPL_ANY=7.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=971
PRIMER_PAIR_11_PENALTY=2.765246
PRIMER_LEFT_11_PENALTY=1.376776
PRIMER_RIGHT_11_PENALTY=1.388470
PRIMER_LEFT_11_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_11_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_11=665,21
PRIMER_RIGHT_11=1633,21
PRIMER_LEFT_11_TM=59.623
PRIMER_RIGHT_11_TM=60.388
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=57.143
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.8000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=969
PRIMER_PAIR_12_PENALTY=3.021038
PRIMER_LEFT_12_PENALTY=1.747794
PRIMER_RIGHT_12_PENALTY=1.273245
PRIMER_LEFT_12_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_12_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_12=664,21
PRIMER_RIGHT_12=1635,21
PRIMER_LEFT_12_TM=59.252
PRIMER_RIGHT_12_TM=59.727
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.7000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=972
PRIMER_PAIR_13_PENALTY=3.021038
PRIMER_LEFT_13_PENALTY=1.747794
PRIMER_RIGHT_13_PENALTY=1.273245
PRIMER_LEFT_13_SEQUENCE=AAGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_13_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_13=663,21
PRIMER_RIGHT_13=1635,21
PRIMER_LEFT_13_TM=59.252
PRIMER_RIGHT_13_TM=59.727
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.7000
PRIMER_PAIR_13_COMPL_ANY=8.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=973
PRIMER_PAIR_14_PENALTY=3.044842
PRIMER_LEFT_14_PENALTY=1.771597
PRIMER_RIGHT_14_PENALTY=1.273245
PRIMER_LEFT_14_SEQUENCE=GGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_14_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_14=665,20
PRIMER_RIGHT_14=1635,21
PRIMER_LEFT_14_TM=58.228
PRIMER_RIGHT_14_TM=59.727
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.7000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=971
PRIMER_PAIR_15_PENALTY=3.103873
PRIMER_LEFT_15_PENALTY=2.533373
PRIMER_RIGHT_15_PENALTY=0.570500
PRIMER_LEFT_15_SEQUENCE=AGGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_15_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_15=664,22
PRIMER_RIGHT_15=1632,20
PRIMER_LEFT_15_TM=60.533
PRIMER_RIGHT_15_TM=59.430
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.8000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=969
PRIMER_PAIR_16_PENALTY=3.103873
PRIMER_LEFT_16_PENALTY=2.533373
PRIMER_RIGHT_16_PENALTY=0.570500
PRIMER_LEFT_16_SEQUENCE=AGGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_16_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_16=664,22
PRIMER_RIGHT_16=1634,20
PRIMER_LEFT_16_TM=60.533
PRIMER_RIGHT_16_TM=59.430
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.8000
PRIMER_RIGHT_16_END_STABILITY=8.7000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=971
PRIMER_PAIR_17_PENALTY=3.103873
PRIMER_LEFT_17_PENALTY=2.533373
PRIMER_RIGHT_17_PENALTY=0.570500
PRIMER_LEFT_17_SEQUENCE=AAGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_17_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_17=663,22
PRIMER_RIGHT_17=1632,20
PRIMER_LEFT_17_TM=60.533
PRIMER_RIGHT_17_TM=59.430
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=970
PRIMER_PAIR_18_PENALTY=3.103873
PRIMER_LEFT_18_PENALTY=2.533373
PRIMER_RIGHT_18_PENALTY=0.570500
PRIMER_LEFT_18_SEQUENCE=AAGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_18_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_18=663,22
PRIMER_RIGHT_18=1634,20
PRIMER_LEFT_18_TM=60.533
PRIMER_RIGHT_18_TM=59.430
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=8.7000
PRIMER_PAIR_18_COMPL_ANY=8.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=972
PRIMER_PAIR_19_PENALTY=3.136263
PRIMER_LEFT_19_PENALTY=1.747794
PRIMER_RIGHT_19_PENALTY=1.388470
PRIMER_LEFT_19_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_19_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_19=664,21
PRIMER_RIGHT_19=1633,21
PRIMER_LEFT_19_TM=59.252
PRIMER_RIGHT_19_TM=60.388
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=57.143
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=970
PRIMER_PAIR_20_PENALTY=3.136263
PRIMER_LEFT_20_PENALTY=1.747794
PRIMER_RIGHT_20_PENALTY=1.388470
PRIMER_LEFT_20_SEQUENCE=AAGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_20_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_20=663,21
PRIMER_RIGHT_20=1633,21
PRIMER_LEFT_20_TM=59.252
PRIMER_RIGHT_20_TM=60.388
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=57.143
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=7.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=971
PRIMER_PAIR_21_PENALTY=3.160067
PRIMER_LEFT_21_PENALTY=1.771597
PRIMER_RIGHT_21_PENALTY=1.388470
PRIMER_LEFT_21_SEQUENCE=GGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_21_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_21=665,20
PRIMER_RIGHT_21=1633,21
PRIMER_LEFT_21_TM=58.228
PRIMER_RIGHT_21_TM=60.388
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=57.143
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=969
PRIMER_PAIR_22_PENALTY=3.447428
PRIMER_LEFT_22_PENALTY=2.174183
PRIMER_RIGHT_22_PENALTY=1.273245
PRIMER_LEFT_22_SEQUENCE=AGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_22_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_22=664,20
PRIMER_RIGHT_22=1635,21
PRIMER_LEFT_22_TM=57.826
PRIMER_RIGHT_22_TM=59.727
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=8.7000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=972
PRIMER_PAIR_23_PENALTY=3.447493
PRIMER_LEFT_23_PENALTY=2.876993
PRIMER_RIGHT_23_PENALTY=0.570500
PRIMER_LEFT_23_SEQUENCE=GGAACTTGCAGAGAAAGCAAAA
PRIMER_RIGHT_23_SEQUENCE=TCGACCACTTCCTTAGCCTC
PRIMER_LEFT_23=665,22
PRIMER_RIGHT_23=1632,20
PRIMER_LEFT_23_TM=60.877
PRIMER_RIGHT_23_TM=59.430
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=968
PRIMER_PAIR_24_PENALTY=3.447493
PRIMER_LEFT_24_PENALTY=2.876993
PRIMER_RIGHT_24_PENALTY=0.570500
PRIMER_LEFT_24_SEQUENCE=GGAACTTGCAGAGAAAGCAAAA
PRIMER_RIGHT_24_SEQUENCE=TCTCGACCACTTCCTTAGCC
PRIMER_LEFT_24=665,22
PRIMER_RIGHT_24=1634,20
PRIMER_LEFT_24_TM=60.877
PRIMER_RIGHT_24_TM=59.430
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=8.7000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=970
PRIMER_PAIR_25_PENALTY=3.562652
PRIMER_LEFT_25_PENALTY=2.174183
PRIMER_RIGHT_25_PENALTY=1.388470
PRIMER_LEFT_25_SEQUENCE=AGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_25_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_25=664,20
PRIMER_RIGHT_25=1633,21
PRIMER_LEFT_25_TM=57.826
PRIMER_RIGHT_25_TM=60.388
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=57.143
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=970
PRIMER_PAIR_26_PENALTY=3.631549
PRIMER_LEFT_26_PENALTY=1.376776
PRIMER_RIGHT_26_PENALTY=2.254773
PRIMER_LEFT_26_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_26_SEQUENCE=AGAGTGAATCTCGACCACTTCC
PRIMER_LEFT_26=665,21
PRIMER_RIGHT_26=1642,22
PRIMER_LEFT_26_TM=59.623
PRIMER_RIGHT_26_TM=59.745
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.8000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=978
PRIMER_PAIR_27_PENALTY=3.644855
PRIMER_LEFT_27_PENALTY=1.376776
PRIMER_RIGHT_27_PENALTY=2.268079
PRIMER_LEFT_27_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_27_SEQUENCE=GAGTGAATCTCGACCACTTCC
PRIMER_LEFT_27=665,21
PRIMER_RIGHT_27=1641,21
PRIMER_LEFT_27_TM=59.623
PRIMER_RIGHT_27_TM=58.732
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.8000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=977
PRIMER_PAIR_28_PENALTY=3.697497
PRIMER_LEFT_28_PENALTY=1.376776
PRIMER_RIGHT_28_PENALTY=2.320721
PRIMER_LEFT_28_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_28_SEQUENCE=TGCTGTCTGAAGAGGAGAGTGA
PRIMER_LEFT_28=665,21
PRIMER_RIGHT_28=1657,22
PRIMER_LEFT_28_TM=59.623
PRIMER_RIGHT_28_TM=60.321
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.8000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=993
PRIMER_PAIR_29_PENALTY=3.806617
PRIMER_LEFT_29_PENALTY=2.533373
PRIMER_RIGHT_29_PENALTY=1.273245
PRIMER_LEFT_29_SEQUENCE=AGGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_29_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_29=664,22
PRIMER_RIGHT_29=1635,21
PRIMER_LEFT_29_TM=60.533
PRIMER_RIGHT_29_TM=59.727
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.8000
PRIMER_RIGHT_29_END_STABILITY=8.7000
PRIMER_PAIR_29_COMPL_ANY=7.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=972
PRIMER_PAIR_30_PENALTY=3.806617
PRIMER_LEFT_30_PENALTY=2.533373
PRIMER_RIGHT_30_PENALTY=1.273245
PRIMER_LEFT_30_SEQUENCE=AAGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_30_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_30=663,22
PRIMER_RIGHT_30=1635,21
PRIMER_LEFT_30_TM=60.533
PRIMER_RIGHT_30_TM=59.727
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.7000
PRIMER_PAIR_30_COMPL_ANY=8.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=973
PRIMER_PAIR_31_PENALTY=3.921842
PRIMER_LEFT_31_PENALTY=2.533373
PRIMER_RIGHT_31_PENALTY=1.388470
PRIMER_LEFT_31_SEQUENCE=AGGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_31_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_31=664,22
PRIMER_RIGHT_31=1633,21
PRIMER_LEFT_31_TM=60.533
PRIMER_RIGHT_31_TM=60.388
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=57.143
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.8000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=970
PRIMER_PAIR_32_PENALTY=3.921842
PRIMER_LEFT_32_PENALTY=2.533373
PRIMER_RIGHT_32_PENALTY=1.388470
PRIMER_LEFT_32_SEQUENCE=AAGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_32_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_32=663,22
PRIMER_RIGHT_32=1633,21
PRIMER_LEFT_32_TM=60.533
PRIMER_RIGHT_32_TM=60.388
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=57.143
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=971
PRIMER_PAIR_33_PENALTY=3.981535
PRIMER_LEFT_33_PENALTY=1.376776
PRIMER_RIGHT_33_PENALTY=2.604759
PRIMER_LEFT_33_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_33_SEQUENCE=CTGCTGTCTGAAGAGGAGAGTG
PRIMER_LEFT_33=665,21
PRIMER_RIGHT_33=1658,22
PRIMER_LEFT_33_TM=59.623
PRIMER_RIGHT_33_TM=59.395
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=54.545
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.8000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=994
PRIMER_PAIR_34_PENALTY=4.002567
PRIMER_LEFT_34_PENALTY=1.747794
PRIMER_RIGHT_34_PENALTY=2.254773
PRIMER_LEFT_34_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_34_SEQUENCE=AGAGTGAATCTCGACCACTTCC
PRIMER_LEFT_34=664,21
PRIMER_RIGHT_34=1642,22
PRIMER_LEFT_34_TM=59.252
PRIMER_RIGHT_34_TM=59.745
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=979
PRIMER_PAIR_35_PENALTY=4.015872
PRIMER_LEFT_35_PENALTY=1.747794
PRIMER_RIGHT_35_PENALTY=2.268079
PRIMER_LEFT_35_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_35_SEQUENCE=GAGTGAATCTCGACCACTTCC
PRIMER_LEFT_35=664,21
PRIMER_RIGHT_35=1641,21
PRIMER_LEFT_35_TM=59.252
PRIMER_RIGHT_35_TM=58.732
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=978
PRIMER_PAIR_36_PENALTY=4.026370
PRIMER_LEFT_36_PENALTY=1.771597
PRIMER_RIGHT_36_PENALTY=2.254773
PRIMER_LEFT_36_SEQUENCE=GGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_36_SEQUENCE=AGAGTGAATCTCGACCACTTCC
PRIMER_LEFT_36=665,20
PRIMER_RIGHT_36=1642,22
PRIMER_LEFT_36_TM=58.228
PRIMER_RIGHT_36_TM=59.745
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=978
PRIMER_PAIR_37_PENALTY=4.039676
PRIMER_LEFT_37_PENALTY=1.771597
PRIMER_RIGHT_37_PENALTY=2.268079
PRIMER_LEFT_37_SEQUENCE=GGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_37_SEQUENCE=GAGTGAATCTCGACCACTTCC
PRIMER_LEFT_37=665,20
PRIMER_RIGHT_37=1641,21
PRIMER_LEFT_37_TM=58.228
PRIMER_RIGHT_37_TM=58.732
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=977
PRIMER_PAIR_38_PENALTY=4.058705
PRIMER_LEFT_38_PENALTY=1.376776
PRIMER_RIGHT_38_PENALTY=2.681929
PRIMER_LEFT_38_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_38_SEQUENCE=TGCTGTCTGAAGAGGAGAGTG
PRIMER_LEFT_38=665,21
PRIMER_RIGHT_38=1657,21
PRIMER_LEFT_38_TM=59.623
PRIMER_RIGHT_38_TM=58.318
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.8000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=993
PRIMER_PAIR_39_PENALTY=4.058705
PRIMER_LEFT_39_PENALTY=1.376776
PRIMER_RIGHT_39_PENALTY=2.681929
PRIMER_LEFT_39_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_39_SEQUENCE=CACTGCTGTCTGAAGAGGAGA
PRIMER_LEFT_39=665,21
PRIMER_RIGHT_39=1660,21
PRIMER_LEFT_39_TM=59.623
PRIMER_RIGHT_39_TM=58.318
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.8000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=996
PRIMER_PAIR_40_PENALTY=4.068515
PRIMER_LEFT_40_PENALTY=1.747794
PRIMER_RIGHT_40_PENALTY=2.320721
PRIMER_LEFT_40_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_40_SEQUENCE=TGCTGTCTGAAGAGGAGAGTGA
PRIMER_LEFT_40=664,21
PRIMER_RIGHT_40=1657,22
PRIMER_LEFT_40_TM=59.252
PRIMER_RIGHT_40_TM=60.321
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=994
PRIMER_PAIR_41_PENALTY=4.068515
PRIMER_LEFT_41_PENALTY=1.747794
PRIMER_RIGHT_41_PENALTY=2.320721
PRIMER_LEFT_41_SEQUENCE=AAGGAACTTGCAGAGAAAGCA
PRIMER_RIGHT_41_SEQUENCE=TGCTGTCTGAAGAGGAGAGTGA
PRIMER_LEFT_41=663,21
PRIMER_RIGHT_41=1657,22
PRIMER_LEFT_41_TM=59.252
PRIMER_RIGHT_41_TM=60.321
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=995
PRIMER_PAIR_42_PENALTY=4.084745
PRIMER_LEFT_42_PENALTY=1.376776
PRIMER_RIGHT_42_PENALTY=2.707969
PRIMER_LEFT_42_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_42_SEQUENCE=TGAATCTCGACCACTTCCTT
PRIMER_LEFT_42=665,21
PRIMER_RIGHT_42=1638,20
PRIMER_LEFT_42_TM=59.623
PRIMER_RIGHT_42_TM=57.292
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.8000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=974
PRIMER_PAIR_43_PENALTY=4.092318
PRIMER_LEFT_43_PENALTY=1.771597
PRIMER_RIGHT_43_PENALTY=2.320721
PRIMER_LEFT_43_SEQUENCE=GGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_43_SEQUENCE=TGCTGTCTGAAGAGGAGAGTGA
PRIMER_LEFT_43=665,20
PRIMER_RIGHT_43=1657,22
PRIMER_LEFT_43_TM=58.228
PRIMER_RIGHT_43_TM=60.321
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=993
PRIMER_PAIR_44_PENALTY=4.122793
PRIMER_LEFT_44_PENALTY=1.376776
PRIMER_RIGHT_44_PENALTY=2.746017
PRIMER_LEFT_44_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_44_SEQUENCE=AGTGAATCTCGACCACTTCCTT
PRIMER_LEFT_44=665,21
PRIMER_RIGHT_44=1640,22
PRIMER_LEFT_44_TM=59.623
PRIMER_RIGHT_44_TM=59.254
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=45.455
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.8000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=976
PRIMER_PAIR_45_PENALTY=4.135121
PRIMER_LEFT_45_PENALTY=1.376776
PRIMER_RIGHT_45_PENALTY=2.758345
PRIMER_LEFT_45_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_45_SEQUENCE=GTGAATCTCGACCACTTCCTT
PRIMER_LEFT_45=665,21
PRIMER_RIGHT_45=1639,21
PRIMER_LEFT_45_TM=59.623
PRIMER_RIGHT_45_TM=58.242
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.8000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=975
PRIMER_PAIR_46_PENALTY=4.150238
PRIMER_LEFT_46_PENALTY=2.876993
PRIMER_RIGHT_46_PENALTY=1.273245
PRIMER_LEFT_46_SEQUENCE=GGAACTTGCAGAGAAAGCAAAA
PRIMER_RIGHT_46_SEQUENCE=ATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_46=665,22
PRIMER_RIGHT_46=1635,21
PRIMER_LEFT_46_TM=60.877
PRIMER_RIGHT_46_TM=59.727
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.6000
PRIMER_RIGHT_46_END_STABILITY=8.7000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=971
PRIMER_PAIR_47_PENALTY=4.265463
PRIMER_LEFT_47_PENALTY=2.876993
PRIMER_RIGHT_47_PENALTY=1.388470
PRIMER_LEFT_47_SEQUENCE=GGAACTTGCAGAGAAAGCAAAA
PRIMER_RIGHT_47_SEQUENCE=CTCGACCACTTCCTTAGCCTC
PRIMER_LEFT_47=665,22
PRIMER_RIGHT_47=1633,21
PRIMER_LEFT_47_TM=60.877
PRIMER_RIGHT_47_TM=60.388
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=57.143
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=969
PRIMER_PAIR_48_PENALTY=4.341373
PRIMER_LEFT_48_PENALTY=1.376776
PRIMER_RIGHT_48_PENALTY=2.964597
PRIMER_LEFT_48_SEQUENCE=GGAACTTGCAGAGAAAGCAAA
PRIMER_RIGHT_48_SEQUENCE=AATCTCGACCACTTCCTTAGCC
PRIMER_LEFT_48=665,21
PRIMER_RIGHT_48=1636,22
PRIMER_LEFT_48_TM=59.623
PRIMER_RIGHT_48_TM=60.965
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.8000
PRIMER_RIGHT_48_END_STABILITY=8.7000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=972
PRIMER_PAIR_49_PENALTY=4.352553
PRIMER_LEFT_49_PENALTY=1.747794
PRIMER_RIGHT_49_PENALTY=2.604759
PRIMER_LEFT_49_SEQUENCE=AGGAACTTGCAGAGAAAGCAA
PRIMER_RIGHT_49_SEQUENCE=CTGCTGTCTGAAGAGGAGAGTG
PRIMER_LEFT_49=664,21
PRIMER_RIGHT_49=1658,22
PRIMER_LEFT_49_TM=59.252
PRIMER_RIGHT_49_TM=59.395
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=54.545
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=995
=
SEQUENCE_ID=MAGIC.338_3_17426754_17426884_hi-0_kn-0_40917_535186_TRUE
SEQUENCE_TARGET=950,230
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAACACGGAGAGGGTAATAGACAAATGTGGATCCATATNGAAATCCNGAACAATAATCTGNNNATCCCATGANCTTCAGGTATCGTATTTTTCATNGCATATCGTNTAGATCTGGGATTGAGNGTAGAATTTCAGACATTGTGCACCCNTTGNTGTGAACAAATGTGNGGACAATNCTAAGCCACATGTCACTATTTNCTTGNTNGTCTTCNNACCATTAANACCCNATCTCCCAAACTAACACTNTTCTTTAACACACACACNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_16679258_16679269_can-0_ler-0_2666_18517_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_22187581_22187889_oy-0_mt-0_46615_29514_TRUE
SEQUENCE_TARGET=950,408
SEQUENCE_TEMPLATE=CAGTTAATGGATAGAATCTTGATAGCAATTTGTCAGAGATTCTTTTNAAAAAGTTGTAATNTTTTTGCAGGCAACTGGAATCTACTACTAAACGCTTGTAACGCCCATACTTTCATTAGATAAGAAAACAAACAATTAAAAGCTCAAATCTTTATGATTGTGATTTTCTCACTTTCCTTATTTTATTGCTTGCTTACACGAGAAGGCACGTTGTNGCAGCATCAACATTTAAAATGCCACTAAAAAAATAAATACGTCGCCTGACAGATTCGAACTCTCGCGNGGAAACCCCATGTACTTAGCAGGNACACNCCTTAACCACTCGNCCAAANCGACNTTTGANGTNTNTACACGTTATTANATATTTAAATGTGACTACTTTGCTATACATTTGTTTGTCAACTGCCCTCATATATCAAANATTAGAATACATCATANGAGATTACAATAAACAAATGTACCGTATATAACTTANATAGTATCGCAGATTCGATGTTTGGCAATTACTTGGTGACTACATCCAACGTTGTTCGTTTGAATATCACGATGATCAATGATAGAAAAAAAGATTACGTNTTAAAATATGGTAATTAAGAGCGACCGCNNNNNNNNNNNNNNTAGAAAATGTTGTATATAATTTGATTACTTGTTGCAGTTGTCAAACGAACAAATCTGTGNTTTTTNTNATCTAATAGAGGTATAGCAAAGTAGTTATCATTCACACATATGCATACTTATTATAATCAAACAATATTATCACTAGCAAAGTCCAATTAGTAAGTGCAAAGACTTGTAATAAACTTCAAAGAATAGAGAGACCCACTACCAAACCTTCCGAATAAAGCCAAGTAAAATGGTAGGCCCNNTATAAACCAACTTTAATCCATTNTAACTTTTATATATTAAAAAAGGGCCTTGAAAGGCTTTGAAACCAATCATAAACCCATTATCACTAGTACTCAACCACAACCACTAACTTTNGTTTCTCCTTTCCNNNTTTNNTTCTTTCACATTTTCTCTTTCNNTCTCTCACAACCACCACCACCACNTCTCAATGTCGTCTTCCACCACCACAACAACCACCGNCCACGGCGAGCCTGAACGACGAACTTACTGGTGTCATGAATGCGATATGAGTTTATCCCTCCTCTCCTCNTCCGATTCCGATTCCGATTCCTCTCCTCTGTTATGTCCCCAATGCCGTGTCGATTTCCTCGAACGTATGGATCACGATTCTTCTTCTTCTAATCTCTTTGACGTTACCATCGGTGATTTTGAAGAACAAGACGGAGAAAACGATGATGAAGANGACGAAGAAGATTGGTGTTTCGTTGATCCAGCTGTTAATTCCGATGATAATTTCCTCCTCGATAGTCCTTACCTTCACCGTCTTCTTCGTCATCTCGCTTCCGATAATTCAGGATCGTCTTCTTCTTCCTCGTCTTCATCTTCTTCTTCGTTATTGAAATCTTCAGATATCGATTCGATCCCCACGATTCAGATCTCGTCTTCTTTGCTCTGTTCCACCGATGATTCAGATCCAGATTCCGTTTTACTCTGCGCCGTTTGCAAAGAGGATTTCNTCNTCGGAGAATCTGCTCGGAGATTNCCGTGTAGTCATATATACCACTCNGATTGCATCGTCCCTTGGCTNTCGGATCATAACTCGTGTCCGCTTTGNAGATTTGAGCTCCCTACGACGGCGAAGGTGGGAATCGGTGGCTCAGAGGCTGANATGAGAATCNGATTATCGGATCTGGCTACGATTGCTGCAGATGGTGATGATGTGGAAGATGATTGGCTTGGAATCAGAAACGCGTTGAGAAGACTGGCTCGTCGTCATGAGCAGATGAGATTGGGAGTGGGAGAGATGGAGAGGAATTTGGCTAGGACTGTTTCAGGTCTTGGAATTGGTATNAGAAGAAGAGAAGAGATTGAAGCAGACAGAANNAACGTCACAACGACGCCTTTGTAAGAACGATTTCGAAATTCAAATATGAGNTTTTTTTTTGTTTGCTTCAAATTCTCAGCTCTCTGTTTATTTNACNGATCGGCGTTTTNATCGATTATTGATTATNGTTACAATAACGAGANTTNTTTTTNNNNNTTCATGTTCAATTGTTTTTACTTTTNAGACAAATGTTGATGATCTCTTTGCTTTTGAGTNTTGTCTACTTTCGATACAATNATTGATGAAATAAACGTAAGTGTAATGATTTGTTCTACGTTTTTCATTTCTCGACTTTCTGATACGGTGAGTGGTGACTGTAGTCTGNTGATCTGGTTGACTCTAATAAAAATAAAAGTG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.158873
PRIMER_LEFT_0_PENALTY=1.002812
PRIMER_RIGHT_0_PENALTY=0.156061
PRIMER_LEFT_0_SEQUENCE=ATCGCAGATTCGATGTTTGG
PRIMER_RIGHT_0_SEQUENCE=GACGAAGAAGACGGTGAAGG
PRIMER_LEFT_0=480,20
PRIMER_RIGHT_0=1399,20
PRIMER_LEFT_0_TM=61.003
PRIMER_RIGHT_0_TM=59.844
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=920
PRIMER_PAIR_1_PENALTY=1.202059
PRIMER_LEFT_1_PENALTY=1.002812
PRIMER_RIGHT_1_PENALTY=0.199246
PRIMER_LEFT_1_SEQUENCE=ATCGCAGATTCGATGTTTGG
PRIMER_RIGHT_1_SEQUENCE=CCTGAATTATCGGAAGCGAG
PRIMER_LEFT_1=480,20
PRIMER_RIGHT_1=1421,20
PRIMER_LEFT_1_TM=61.003
PRIMER_RIGHT_1_TM=59.801
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=942
PRIMER_PAIR_2_PENALTY=1.319824
PRIMER_LEFT_2_PENALTY=1.306217
PRIMER_RIGHT_2_PENALTY=0.013607
PRIMER_LEFT_2_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_2_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_2=828,20
PRIMER_RIGHT_2=1807,20
PRIMER_LEFT_2_TM=58.694
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=980
PRIMER_PAIR_3_PENALTY=1.319824
PRIMER_LEFT_3_PENALTY=1.306217
PRIMER_RIGHT_3_PENALTY=0.013607
PRIMER_LEFT_3_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_3_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_3=827,20
PRIMER_RIGHT_3=1807,20
PRIMER_LEFT_3_TM=58.694
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=981
PRIMER_PAIR_4_PENALTY=1.319824
PRIMER_LEFT_4_PENALTY=1.306217
PRIMER_RIGHT_4_PENALTY=0.013607
PRIMER_LEFT_4_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_4_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_4=826,20
PRIMER_RIGHT_4=1807,20
PRIMER_LEFT_4_TM=58.694
PRIMER_RIGHT_4_TM=60.014
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=982
PRIMER_PAIR_5_PENALTY=1.396481
PRIMER_LEFT_5_PENALTY=1.382873
PRIMER_RIGHT_5_PENALTY=0.013607
PRIMER_LEFT_5_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_5_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_5=828,21
PRIMER_RIGHT_5=1807,20
PRIMER_LEFT_5_TM=59.617
PRIMER_RIGHT_5_TM=60.014
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=980
PRIMER_PAIR_6_PENALTY=1.462278
PRIMER_LEFT_6_PENALTY=1.306217
PRIMER_RIGHT_6_PENALTY=0.156061
PRIMER_LEFT_6_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_6_SEQUENCE=GACGAAGAAGACGGTGAAGG
PRIMER_LEFT_6=828,20
PRIMER_RIGHT_6=1399,20
PRIMER_LEFT_6_TM=58.694
PRIMER_RIGHT_6_TM=59.844
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.0000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=572
PRIMER_PAIR_7_PENALTY=1.462278
PRIMER_LEFT_7_PENALTY=1.306217
PRIMER_RIGHT_7_PENALTY=0.156061
PRIMER_LEFT_7_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_7_SEQUENCE=GACGAAGAAGACGGTGAAGG
PRIMER_LEFT_7=827,20
PRIMER_RIGHT_7=1399,20
PRIMER_LEFT_7_TM=58.694
PRIMER_RIGHT_7_TM=59.844
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=573
PRIMER_PAIR_8_PENALTY=1.462278
PRIMER_LEFT_8_PENALTY=1.306217
PRIMER_RIGHT_8_PENALTY=0.156061
PRIMER_LEFT_8_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_8_SEQUENCE=GACGAAGAAGACGGTGAAGG
PRIMER_LEFT_8=826,20
PRIMER_RIGHT_8=1399,20
PRIMER_LEFT_8_TM=58.694
PRIMER_RIGHT_8_TM=59.844
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=574
PRIMER_PAIR_9_PENALTY=1.469875
PRIMER_LEFT_9_PENALTY=1.306217
PRIMER_RIGHT_9_PENALTY=0.163658
PRIMER_LEFT_9_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_9_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_9=828,20
PRIMER_RIGHT_9=1701,20
PRIMER_LEFT_9_TM=58.694
PRIMER_RIGHT_9_TM=59.836
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=10.0000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=874
PRIMER_PAIR_10_PENALTY=1.469875
PRIMER_LEFT_10_PENALTY=1.306217
PRIMER_RIGHT_10_PENALTY=0.163658
PRIMER_LEFT_10_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_10_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_10=827,20
PRIMER_RIGHT_10=1701,20
PRIMER_LEFT_10_TM=58.694
PRIMER_RIGHT_10_TM=59.836
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=875
PRIMER_PAIR_11_PENALTY=1.469875
PRIMER_LEFT_11_PENALTY=1.306217
PRIMER_RIGHT_11_PENALTY=0.163658
PRIMER_LEFT_11_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_11_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_11=826,20
PRIMER_RIGHT_11=1701,20
PRIMER_LEFT_11_TM=58.694
PRIMER_RIGHT_11_TM=59.836
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=876
PRIMER_PAIR_12_PENALTY=1.485474
PRIMER_LEFT_12_PENALTY=1.002812
PRIMER_RIGHT_12_PENALTY=0.482662
PRIMER_LEFT_12_SEQUENCE=ATCGCAGATTCGATGTTTGG
PRIMER_RIGHT_12_SEQUENCE=GGAAGCGAGATGACGAAGAA
PRIMER_LEFT_12=480,20
PRIMER_RIGHT_12=1410,20
PRIMER_LEFT_12_TM=61.003
PRIMER_RIGHT_12_TM=60.483
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.8000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=931
PRIMER_PAIR_13_PENALTY=1.505463
PRIMER_LEFT_13_PENALTY=1.306217
PRIMER_RIGHT_13_PENALTY=0.199246
PRIMER_LEFT_13_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_13_SEQUENCE=CCTGAATTATCGGAAGCGAG
PRIMER_LEFT_13=828,20
PRIMER_RIGHT_13=1421,20
PRIMER_LEFT_13_TM=58.694
PRIMER_RIGHT_13_TM=59.801
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=8.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=594
PRIMER_PAIR_14_PENALTY=1.505463
PRIMER_LEFT_14_PENALTY=1.306217
PRIMER_RIGHT_14_PENALTY=0.199246
PRIMER_LEFT_14_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_14_SEQUENCE=CCTGAATTATCGGAAGCGAG
PRIMER_LEFT_14=827,20
PRIMER_RIGHT_14=1421,20
PRIMER_LEFT_14_TM=58.694
PRIMER_RIGHT_14_TM=59.801
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=8.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=595
PRIMER_PAIR_15_PENALTY=1.505463
PRIMER_LEFT_15_PENALTY=1.306217
PRIMER_RIGHT_15_PENALTY=0.199246
PRIMER_LEFT_15_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_15_SEQUENCE=CCTGAATTATCGGAAGCGAG
PRIMER_LEFT_15=826,20
PRIMER_RIGHT_15=1421,20
PRIMER_LEFT_15_TM=58.694
PRIMER_RIGHT_15_TM=59.801
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=8.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=596
PRIMER_PAIR_16_PENALTY=1.538934
PRIMER_LEFT_16_PENALTY=1.382873
PRIMER_RIGHT_16_PENALTY=0.156061
PRIMER_LEFT_16_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_16_SEQUENCE=GACGAAGAAGACGGTGAAGG
PRIMER_LEFT_16=828,21
PRIMER_RIGHT_16=1399,20
PRIMER_LEFT_16_TM=59.617
PRIMER_RIGHT_16_TM=59.844
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=572
PRIMER_PAIR_17_PENALTY=1.546532
PRIMER_LEFT_17_PENALTY=1.382873
PRIMER_RIGHT_17_PENALTY=0.163658
PRIMER_LEFT_17_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_17_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_17=828,21
PRIMER_RIGHT_17=1701,20
PRIMER_LEFT_17_TM=59.617
PRIMER_RIGHT_17_TM=59.836
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=874
PRIMER_PAIR_18_PENALTY=1.560830
PRIMER_LEFT_18_PENALTY=1.306217
PRIMER_RIGHT_18_PENALTY=0.254613
PRIMER_LEFT_18_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_18_SEQUENCE=ATCGGTGGAACAGAGCAAAG
PRIMER_LEFT_18=828,20
PRIMER_RIGHT_18=1530,20
PRIMER_LEFT_18_TM=58.694
PRIMER_RIGHT_18_TM=60.255
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=10.0000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=703
PRIMER_PAIR_19_PENALTY=1.560830
PRIMER_LEFT_19_PENALTY=1.306217
PRIMER_RIGHT_19_PENALTY=0.254613
PRIMER_LEFT_19_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_19_SEQUENCE=ATCGGTGGAACAGAGCAAAG
PRIMER_LEFT_19=827,20
PRIMER_RIGHT_19=1530,20
PRIMER_LEFT_19_TM=58.694
PRIMER_RIGHT_19_TM=60.255
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=704
PRIMER_PAIR_20_PENALTY=1.560830
PRIMER_LEFT_20_PENALTY=1.306217
PRIMER_RIGHT_20_PENALTY=0.254613
PRIMER_LEFT_20_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_20_SEQUENCE=ATCGGTGGAACAGAGCAAAG
PRIMER_LEFT_20=826,20
PRIMER_RIGHT_20=1530,20
PRIMER_LEFT_20_TM=58.694
PRIMER_RIGHT_20_TM=60.255
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=705
PRIMER_PAIR_21_PENALTY=1.582120
PRIMER_LEFT_21_PENALTY=1.382873
PRIMER_RIGHT_21_PENALTY=0.199246
PRIMER_LEFT_21_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_21_SEQUENCE=CCTGAATTATCGGAAGCGAG
PRIMER_LEFT_21=828,21
PRIMER_RIGHT_21=1421,20
PRIMER_LEFT_21_TM=59.617
PRIMER_RIGHT_21_TM=59.801
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=9.9000
PRIMER_PAIR_21_COMPL_ANY=8.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=594
PRIMER_PAIR_22_PENALTY=1.637487
PRIMER_LEFT_22_PENALTY=1.382873
PRIMER_RIGHT_22_PENALTY=0.254613
PRIMER_LEFT_22_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_22_SEQUENCE=ATCGGTGGAACAGAGCAAAG
PRIMER_LEFT_22=828,21
PRIMER_RIGHT_22=1530,20
PRIMER_LEFT_22_TM=59.617
PRIMER_RIGHT_22_TM=60.255
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=703
PRIMER_PAIR_23_PENALTY=1.658027
PRIMER_LEFT_23_PENALTY=1.306217
PRIMER_RIGHT_23_PENALTY=0.351810
PRIMER_LEFT_23_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_23_SEQUENCE=CAAGCCAATCATCTTCCACA
PRIMER_LEFT_23=828,20
PRIMER_RIGHT_23=1804,20
PRIMER_LEFT_23_TM=58.694
PRIMER_RIGHT_23_TM=59.648
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.0000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=977
PRIMER_PAIR_24_PENALTY=1.658027
PRIMER_LEFT_24_PENALTY=1.306217
PRIMER_RIGHT_24_PENALTY=0.351810
PRIMER_LEFT_24_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_24_SEQUENCE=CAAGCCAATCATCTTCCACA
PRIMER_LEFT_24=827,20
PRIMER_RIGHT_24=1804,20
PRIMER_LEFT_24_TM=58.694
PRIMER_RIGHT_24_TM=59.648
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=978
PRIMER_PAIR_25_PENALTY=1.658027
PRIMER_LEFT_25_PENALTY=1.306217
PRIMER_RIGHT_25_PENALTY=0.351810
PRIMER_LEFT_25_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_25_SEQUENCE=CAAGCCAATCATCTTCCACA
PRIMER_LEFT_25=826,20
PRIMER_RIGHT_25=1804,20
PRIMER_LEFT_25_TM=58.694
PRIMER_RIGHT_25_TM=59.648
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.7000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=979
PRIMER_PAIR_26_PENALTY=1.658139
PRIMER_LEFT_26_PENALTY=1.644532
PRIMER_RIGHT_26_PENALTY=0.013607
PRIMER_LEFT_26_SEQUENCE=AGACCCACTACCAAACCTTCC
PRIMER_RIGHT_26_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_26=815,21
PRIMER_RIGHT_26=1807,20
PRIMER_LEFT_26_TM=59.355
PRIMER_RIGHT_26_TM=60.014
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=993
PRIMER_PAIR_27_PENALTY=1.663245
PRIMER_LEFT_27_PENALTY=1.649638
PRIMER_RIGHT_27_PENALTY=0.013607
PRIMER_LEFT_27_SEQUENCE=GACCCACTACCAAACCTTCC
PRIMER_RIGHT_27_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_27=816,20
PRIMER_RIGHT_27=1807,20
PRIMER_LEFT_27_TM=58.350
PRIMER_RIGHT_27_TM=60.014
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=992
PRIMER_PAIR_28_PENALTY=1.685872
PRIMER_LEFT_28_PENALTY=1.672265
PRIMER_RIGHT_28_PENALTY=0.013607
PRIMER_LEFT_28_SEQUENCE=CCTTCCGAATAAAGCCAAGT
PRIMER_RIGHT_28_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_28=830,20
PRIMER_RIGHT_28=1807,20
PRIMER_LEFT_28_TM=58.328
PRIMER_RIGHT_28_TM=60.014
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=978
PRIMER_PAIR_29_PENALTY=1.685872
PRIMER_LEFT_29_PENALTY=1.672265
PRIMER_RIGHT_29_PENALTY=0.013607
PRIMER_LEFT_29_SEQUENCE=ACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_29_SEQUENCE=TTCCAAGCCAATCATCTTCC
PRIMER_LEFT_29=829,20
PRIMER_RIGHT_29=1807,20
PRIMER_LEFT_29_TM=58.328
PRIMER_RIGHT_29_TM=60.014
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=979
PRIMER_PAIR_30_PENALTY=1.734684
PRIMER_LEFT_30_PENALTY=1.382873
PRIMER_RIGHT_30_PENALTY=0.351810
PRIMER_LEFT_30_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_30_SEQUENCE=CAAGCCAATCATCTTCCACA
PRIMER_LEFT_30=828,21
PRIMER_RIGHT_30=1804,20
PRIMER_LEFT_30_TM=59.617
PRIMER_RIGHT_30_TM=59.648
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=977
PRIMER_PAIR_31_PENALTY=1.767082
PRIMER_LEFT_31_PENALTY=1.306217
PRIMER_RIGHT_31_PENALTY=0.460865
PRIMER_LEFT_31_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_31_SEQUENCE=CCAAGCCAATCATCTTCCAC
PRIMER_LEFT_31=828,20
PRIMER_RIGHT_31=1805,20
PRIMER_LEFT_31_TM=58.694
PRIMER_RIGHT_31_TM=60.461
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=10.0000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=978
PRIMER_PAIR_32_PENALTY=1.767082
PRIMER_LEFT_32_PENALTY=1.306217
PRIMER_RIGHT_32_PENALTY=0.460865
PRIMER_LEFT_32_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_32_SEQUENCE=CCAAGCCAATCATCTTCCAC
PRIMER_LEFT_32=827,20
PRIMER_RIGHT_32=1805,20
PRIMER_LEFT_32_TM=58.694
PRIMER_RIGHT_32_TM=60.461
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=979
PRIMER_PAIR_33_PENALTY=1.767082
PRIMER_LEFT_33_PENALTY=1.306217
PRIMER_RIGHT_33_PENALTY=0.460865
PRIMER_LEFT_33_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_33_SEQUENCE=CCAAGCCAATCATCTTCCAC
PRIMER_LEFT_33=826,20
PRIMER_RIGHT_33=1805,20
PRIMER_LEFT_33_TM=58.694
PRIMER_RIGHT_33_TM=60.461
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=980
PRIMER_PAIR_34_PENALTY=1.788879
PRIMER_LEFT_34_PENALTY=1.306217
PRIMER_RIGHT_34_PENALTY=0.482662
PRIMER_LEFT_34_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_34_SEQUENCE=GGAAGCGAGATGACGAAGAA
PRIMER_LEFT_34=828,20
PRIMER_RIGHT_34=1410,20
PRIMER_LEFT_34_TM=58.694
PRIMER_RIGHT_34_TM=60.483
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.0000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=583
PRIMER_PAIR_35_PENALTY=1.788879
PRIMER_LEFT_35_PENALTY=1.306217
PRIMER_RIGHT_35_PENALTY=0.482662
PRIMER_LEFT_35_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_35_SEQUENCE=GGAAGCGAGATGACGAAGAA
PRIMER_LEFT_35=827,20
PRIMER_RIGHT_35=1410,20
PRIMER_LEFT_35_TM=58.694
PRIMER_RIGHT_35_TM=60.483
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=584
PRIMER_PAIR_36_PENALTY=1.788879
PRIMER_LEFT_36_PENALTY=1.306217
PRIMER_RIGHT_36_PENALTY=0.482662
PRIMER_LEFT_36_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_36_SEQUENCE=GGAAGCGAGATGACGAAGAA
PRIMER_LEFT_36=826,20
PRIMER_RIGHT_36=1410,20
PRIMER_LEFT_36_TM=58.694
PRIMER_RIGHT_36_TM=60.483
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=585
PRIMER_PAIR_37_PENALTY=1.808191
PRIMER_LEFT_37_PENALTY=1.644532
PRIMER_RIGHT_37_PENALTY=0.163658
PRIMER_LEFT_37_SEQUENCE=AGACCCACTACCAAACCTTCC
PRIMER_RIGHT_37_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_37=815,21
PRIMER_RIGHT_37=1701,20
PRIMER_LEFT_37_TM=59.355
PRIMER_RIGHT_37_TM=59.836
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=887
PRIMER_PAIR_38_PENALTY=1.813297
PRIMER_LEFT_38_PENALTY=1.649638
PRIMER_RIGHT_38_PENALTY=0.163658
PRIMER_LEFT_38_SEQUENCE=GACCCACTACCAAACCTTCC
PRIMER_RIGHT_38_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_38=816,20
PRIMER_RIGHT_38=1701,20
PRIMER_LEFT_38_TM=58.350
PRIMER_RIGHT_38_TM=59.836
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=886
PRIMER_PAIR_39_PENALTY=1.828326
PRIMER_LEFT_39_PENALTY=1.672265
PRIMER_RIGHT_39_PENALTY=0.156061
PRIMER_LEFT_39_SEQUENCE=CCTTCCGAATAAAGCCAAGT
PRIMER_RIGHT_39_SEQUENCE=GACGAAGAAGACGGTGAAGG
PRIMER_LEFT_39=830,20
PRIMER_RIGHT_39=1399,20
PRIMER_LEFT_39_TM=58.328
PRIMER_RIGHT_39_TM=59.844
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=570
PRIMER_PAIR_40_PENALTY=1.828326
PRIMER_LEFT_40_PENALTY=1.672265
PRIMER_RIGHT_40_PENALTY=0.156061
PRIMER_LEFT_40_SEQUENCE=ACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_40_SEQUENCE=GACGAAGAAGACGGTGAAGG
PRIMER_LEFT_40=829,20
PRIMER_RIGHT_40=1399,20
PRIMER_LEFT_40_TM=58.328
PRIMER_RIGHT_40_TM=59.844
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=571
PRIMER_PAIR_41_PENALTY=1.835923
PRIMER_LEFT_41_PENALTY=1.672265
PRIMER_RIGHT_41_PENALTY=0.163658
PRIMER_LEFT_41_SEQUENCE=CCTTCCGAATAAAGCCAAGT
PRIMER_RIGHT_41_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_41=830,20
PRIMER_RIGHT_41=1701,20
PRIMER_LEFT_41_TM=58.328
PRIMER_RIGHT_41_TM=59.836
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=872
PRIMER_PAIR_42_PENALTY=1.835923
PRIMER_LEFT_42_PENALTY=1.672265
PRIMER_RIGHT_42_PENALTY=0.163658
PRIMER_LEFT_42_SEQUENCE=ACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_42_SEQUENCE=CGTCGTAGGGAGCTCAAATC
PRIMER_LEFT_42=829,20
PRIMER_RIGHT_42=1701,20
PRIMER_LEFT_42_TM=58.328
PRIMER_RIGHT_42_TM=59.836
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=873
PRIMER_PAIR_43_PENALTY=1.843739
PRIMER_LEFT_43_PENALTY=1.382873
PRIMER_RIGHT_43_PENALTY=0.460865
PRIMER_LEFT_43_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_43_SEQUENCE=CCAAGCCAATCATCTTCCAC
PRIMER_LEFT_43=828,21
PRIMER_RIGHT_43=1805,20
PRIMER_LEFT_43_TM=59.617
PRIMER_RIGHT_43_TM=60.461
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=978
PRIMER_PAIR_44_PENALTY=1.843779
PRIMER_LEFT_44_PENALTY=1.644532
PRIMER_RIGHT_44_PENALTY=0.199246
PRIMER_LEFT_44_SEQUENCE=AGACCCACTACCAAACCTTCC
PRIMER_RIGHT_44_SEQUENCE=CCTGAATTATCGGAAGCGAG
PRIMER_LEFT_44=815,21
PRIMER_RIGHT_44=1421,20
PRIMER_LEFT_44_TM=59.355
PRIMER_RIGHT_44_TM=59.801
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=607
PRIMER_PAIR_45_PENALTY=1.848885
PRIMER_LEFT_45_PENALTY=1.649638
PRIMER_RIGHT_45_PENALTY=0.199246
PRIMER_LEFT_45_SEQUENCE=GACCCACTACCAAACCTTCC
PRIMER_RIGHT_45_SEQUENCE=CCTGAATTATCGGAAGCGAG
PRIMER_LEFT_45=816,20
PRIMER_RIGHT_45=1421,20
PRIMER_LEFT_45_TM=58.350
PRIMER_RIGHT_45_TM=59.801
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=606
PRIMER_PAIR_46_PENALTY=1.850197
PRIMER_LEFT_46_PENALTY=1.306217
PRIMER_RIGHT_46_PENALTY=0.543980
PRIMER_LEFT_46_SEQUENCE=AACCTTCCGAATAAAGCCAA
PRIMER_RIGHT_46_SEQUENCE=GCCAATCATCTTCCACATCA
PRIMER_LEFT_46=828,20
PRIMER_RIGHT_46=1801,20
PRIMER_LEFT_46_TM=58.694
PRIMER_RIGHT_46_TM=59.456
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=10.0000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=974
PRIMER_PAIR_47_PENALTY=1.850197
PRIMER_LEFT_47_PENALTY=1.306217
PRIMER_RIGHT_47_PENALTY=0.543980
PRIMER_LEFT_47_SEQUENCE=AAACCTTCCGAATAAAGCCA
PRIMER_RIGHT_47_SEQUENCE=GCCAATCATCTTCCACATCA
PRIMER_LEFT_47=827,20
PRIMER_RIGHT_47=1801,20
PRIMER_LEFT_47_TM=58.694
PRIMER_RIGHT_47_TM=59.456
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=975
PRIMER_PAIR_48_PENALTY=1.850197
PRIMER_LEFT_48_PENALTY=1.306217
PRIMER_RIGHT_48_PENALTY=0.543980
PRIMER_LEFT_48_SEQUENCE=CAAACCTTCCGAATAAAGCC
PRIMER_RIGHT_48_SEQUENCE=GCCAATCATCTTCCACATCA
PRIMER_LEFT_48=826,20
PRIMER_RIGHT_48=1801,20
PRIMER_LEFT_48_TM=58.694
PRIMER_RIGHT_48_TM=59.456
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=976
PRIMER_PAIR_49_PENALTY=1.865535
PRIMER_LEFT_49_PENALTY=1.382873
PRIMER_RIGHT_49_PENALTY=0.482662
PRIMER_LEFT_49_SEQUENCE=AACCTTCCGAATAAAGCCAAG
PRIMER_RIGHT_49_SEQUENCE=GGAAGCGAGATGACGAAGAA
PRIMER_LEFT_49=828,21
PRIMER_RIGHT_49=1410,20
PRIMER_LEFT_49_TM=59.617
PRIMER_RIGHT_49_TM=60.483
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=583
=
SEQUENCE_ID=MAGIC.426_3_12266362_12266584_wil-2_zu-0_271273_5086_TRUE
SEQUENCE_TARGET=950,322
SEQUENCE_TEMPLATE=GAAAGAGGATNAAAAAAACGCATATCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_5909760_5910025_po-0_kn-0_137631_6514_TRUE
SEQUENCE_TARGET=950,365
SEQUENCE_TEMPLATE=NTTATTTTGAAATTTCNNTTTCNTNCGGTTNCNGTTCTTTCNGTTTTAAAATTTAGTATNNATTCGGTTATTTTGTTTTGAACAATTTNGNATATTTCAGTTATTGTTNAATATTTTCNNATANTTNTCNGNTANTTTCGANTANNNTTANTATTTTATATANNTTTTNAANTNTTTTTAATTATTNTGTNTAGAAATAACANATAGTTTTGAANATATTATTGTANTGCGGATATTTTANNCTACCCNAAATATTTNGGTTCGGNACCNATTNGNNTTNGNTTATTTNTAAAANTTTCGTNNNNNNNNGGTTCTATTATTTTTGGTGCGGTTCCAATTGGNTACATTCGNNNNNNTNNNTTTGCNNAATTGNNANNNATGAACTNAGAGGTCANNNNNNNTAAANTGCGAANNTNAANAATTGNCNNGTATTCATNCATTTCCCGCNTAAATCCTCCANCTTCTTCTTCGCCGGCNTNAATGGANTCNCCNACACANACGNCNGNNNACATAGAANCCNTGACGGAGTTTCTAGTAAAGCACTACNCCGATCAGCTCCGCGAAATCTCTCTCTCTTCNGANCCCAAACTTCACTACCCTCTCTTCNTCGAGTACGCAGAACTCGTTGACGATAATCCGTCTCTAGCNCGTCNAGTTTTCTCANATCCCGAACANTATCTTCGNCAATTCGACGANTCAGCCATTNTAGCTCACGTATGTACTCAATTTNCCCCNAAATTTGATTATACGAAATGGTGATTTCNANTAGCTTTGGGTGTAGAAAATTGCNNTGGAGCATATGAAGAAATTTNAGNAGAAGATTGGAATTGAGAAGAGATTTNTACATGTTCGTATAAACACNAGTGGATCTCCTCTAGAACGAAGCCCNNNTNTGTGTGNNNNNNTCTCTTGTTTTCGAGTTTGTTCTGCTTCTNTTAGNTGATTTTGATTTTGACACAGAGACNTTCCCGAGTATTGGCCGGGTTAGAGTAAAGCACCGNGGGATNCTGATGATGTTGAAAGGCACNGTGATTCGATCCGGGGCTGTNAAAATGTANGANGGAGAAAAGATGTATCGATGTCGAAAATGCAAGCACATGTNNNNNNNNNNNNNGTGGTTGAAACTGTATATTATNCATTTTNTCATCNNNNNNTNTTGTANTATNGCTGATTGAGTTNNTTTCTTCTATAGGTTTCCTATTTTCCCTGAGTTNGAATCAATCAANTCTATTGTGAAACCNCCNTTTTGTCCTTCTCAGGTTTGTNNNATTTCNTTCTTTGNCTAGCTAANCTNNTTTNNANTAGANNGTNTTCTGTTTTGCGANANCACTCNGACTANGGTCTCTNNNATTGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNACAGCGGTCAAAAGCNTGTGANGGCACAAACTTTGATCCTGTNGATGATACAGTAACACGNCATGATTATCAAGAAATCAAAATCCAAGAAAATACTCAGGTTTTAGGTGTTGGTGTTATCCCAAGGTCNATTCTAGTCGTCCTGAAAGATGACCTTGTTGACAATGTTAAAGCTGGAGGTAAACCAGTTTGTTTGTTTTTNTTCTGTGTCTTACATATNCANNATTGCGTNAATATTATGGCTCAGNGNAANCATTCNAAACGTATCAGCATAGCTGCTCCNCCTCCTGATATAACATATTNCNNTNNATGCAGATGATGTTGTTGTCTCTGGAATCTTGACTTCAAAATGGTCTCATGACTTNAAAGATGTTCGNTGNGACCTTGAACCTATGTTGATTGCCAATCATGTCAGGTAAATGGCTTGATAANTTTGATAGAGACCTACNANTCNATNTTATTAAGGANATCNTTANNANNNTCCTNAAANTTNTATNCANAANNGGTACTAGTTTGTTTGCCTGAAACAATTATTNAGGTCTTTGATGNGATTCTANTTTCTCNTNTTCTTAGTTACTATTNNTCATTANAGTTTNNTATTAACTNNCTTTCTNCCTTNTNTTCACCTGTTATCNTGCCACTNTCNAGAAGAACTAATGAGCTGAAATCAGAAATCGATATATCTGATGATTTGATCGAGAAGTTCAAGAACTTTTGGAGCCACTTCANAGATACCCCTTTGAAAGGTAAAAANNCANTGTTNNCTGNAANNTATTCCGCCTNTTTTNNTNGCNATTATTATNNACANNTATTCCCCTGCCNTNGANAANNNNTACGTATNNNTTTNGTNAANATTTATGCTAAATTCTTTANTGTTTTGGNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.910557
PRIMER_LEFT_0_PENALTY=0.736567
PRIMER_RIGHT_0_PENALTY=2.173990
PRIMER_LEFT_0_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_0_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_0=556,20
PRIMER_RIGHT_0=1507,21
PRIMER_LEFT_0_TM=59.263
PRIMER_RIGHT_0_TM=58.826
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=952
PRIMER_PAIR_1_PENALTY=2.910557
PRIMER_LEFT_1_PENALTY=0.736567
PRIMER_RIGHT_1_PENALTY=2.173990
PRIMER_LEFT_1_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_1=555,20
PRIMER_RIGHT_1=1507,21
PRIMER_LEFT_1_TM=59.263
PRIMER_RIGHT_1_TM=58.826
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=953
PRIMER_PAIR_2_PENALTY=2.968529
PRIMER_LEFT_2_PENALTY=0.736567
PRIMER_RIGHT_2_PENALTY=2.231961
PRIMER_LEFT_2_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_2_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_2=556,20
PRIMER_RIGHT_2=1506,20
PRIMER_LEFT_2_TM=59.263
PRIMER_RIGHT_2_TM=57.768
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=951
PRIMER_PAIR_3_PENALTY=2.968529
PRIMER_LEFT_3_PENALTY=0.736567
PRIMER_RIGHT_3_PENALTY=2.231961
PRIMER_LEFT_3_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_3_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_3=556,20
PRIMER_RIGHT_3=1507,20
PRIMER_LEFT_3_TM=59.263
PRIMER_RIGHT_3_TM=57.768
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=952
PRIMER_PAIR_4_PENALTY=2.968529
PRIMER_LEFT_4_PENALTY=0.736567
PRIMER_RIGHT_4_PENALTY=2.231961
PRIMER_LEFT_4_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_4_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_4=556,20
PRIMER_RIGHT_4=1508,20
PRIMER_LEFT_4_TM=59.263
PRIMER_RIGHT_4_TM=57.768
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=953
PRIMER_PAIR_5_PENALTY=2.968529
PRIMER_LEFT_5_PENALTY=0.736567
PRIMER_RIGHT_5_PENALTY=2.231961
PRIMER_LEFT_5_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_5_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_5=555,20
PRIMER_RIGHT_5=1506,20
PRIMER_LEFT_5_TM=59.263
PRIMER_RIGHT_5_TM=57.768
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=952
PRIMER_PAIR_6_PENALTY=2.968529
PRIMER_LEFT_6_PENALTY=0.736567
PRIMER_RIGHT_6_PENALTY=2.231961
PRIMER_LEFT_6_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_6_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_6=555,20
PRIMER_RIGHT_6=1507,20
PRIMER_LEFT_6_TM=59.263
PRIMER_RIGHT_6_TM=57.768
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=953
PRIMER_PAIR_7_PENALTY=2.968529
PRIMER_LEFT_7_PENALTY=0.736567
PRIMER_RIGHT_7_PENALTY=2.231961
PRIMER_LEFT_7_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_7_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_7=555,20
PRIMER_RIGHT_7=1508,20
PRIMER_LEFT_7_TM=59.263
PRIMER_RIGHT_7_TM=57.768
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=954
PRIMER_PAIR_8_PENALTY=3.053056
PRIMER_LEFT_8_PENALTY=0.736567
PRIMER_RIGHT_8_PENALTY=2.316489
PRIMER_LEFT_8_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_8_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_8=556,20
PRIMER_RIGHT_8=1509,21
PRIMER_LEFT_8_TM=59.263
PRIMER_RIGHT_8_TM=58.684
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=954
PRIMER_PAIR_9_PENALTY=3.053056
PRIMER_LEFT_9_PENALTY=0.736567
PRIMER_RIGHT_9_PENALTY=2.316489
PRIMER_LEFT_9_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_9_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_9=555,20
PRIMER_RIGHT_9=1509,21
PRIMER_LEFT_9_TM=59.263
PRIMER_RIGHT_9_TM=58.684
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=955
PRIMER_PAIR_10_PENALTY=3.128736
PRIMER_LEFT_10_PENALTY=0.736567
PRIMER_RIGHT_10_PENALTY=2.392169
PRIMER_LEFT_10_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_10_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_10=556,20
PRIMER_RIGHT_10=1508,21
PRIMER_LEFT_10_TM=59.263
PRIMER_RIGHT_10_TM=61.392
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=953
PRIMER_PAIR_11_PENALTY=3.128736
PRIMER_LEFT_11_PENALTY=0.736567
PRIMER_RIGHT_11_PENALTY=2.392169
PRIMER_LEFT_11_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_11_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_11=555,20
PRIMER_RIGHT_11=1508,21
PRIMER_LEFT_11_TM=59.263
PRIMER_RIGHT_11_TM=61.392
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=954
PRIMER_PAIR_12_PENALTY=3.253219
PRIMER_LEFT_12_PENALTY=0.736567
PRIMER_RIGHT_12_PENALTY=2.516652
PRIMER_LEFT_12_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_12_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_12=556,20
PRIMER_RIGHT_12=1510,22
PRIMER_LEFT_12_TM=59.263
PRIMER_RIGHT_12_TM=60.517
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=955
PRIMER_PAIR_13_PENALTY=3.253219
PRIMER_LEFT_13_PENALTY=0.736567
PRIMER_RIGHT_13_PENALTY=2.516652
PRIMER_LEFT_13_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_13_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_13=555,20
PRIMER_RIGHT_13=1510,22
PRIMER_LEFT_13_TM=59.263
PRIMER_RIGHT_13_TM=60.517
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=956
PRIMER_PAIR_14_PENALTY=3.319423
PRIMER_LEFT_14_PENALTY=1.145433
PRIMER_RIGHT_14_PENALTY=2.173990
PRIMER_LEFT_14_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_14_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_14=554,20
PRIMER_RIGHT_14=1507,21
PRIMER_LEFT_14_TM=61.145
PRIMER_RIGHT_14_TM=58.826
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=954
PRIMER_PAIR_15_PENALTY=3.377394
PRIMER_LEFT_15_PENALTY=1.145433
PRIMER_RIGHT_15_PENALTY=2.231961
PRIMER_LEFT_15_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_15_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_15=554,20
PRIMER_RIGHT_15=1506,20
PRIMER_LEFT_15_TM=61.145
PRIMER_RIGHT_15_TM=57.768
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=953
PRIMER_PAIR_16_PENALTY=3.377394
PRIMER_LEFT_16_PENALTY=1.145433
PRIMER_RIGHT_16_PENALTY=2.231961
PRIMER_LEFT_16_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_16_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_16=554,20
PRIMER_RIGHT_16=1507,20
PRIMER_LEFT_16_TM=61.145
PRIMER_RIGHT_16_TM=57.768
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=954
PRIMER_PAIR_17_PENALTY=3.377394
PRIMER_LEFT_17_PENALTY=1.145433
PRIMER_RIGHT_17_PENALTY=2.231961
PRIMER_LEFT_17_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_17_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_17=554,20
PRIMER_RIGHT_17=1508,20
PRIMER_LEFT_17_TM=61.145
PRIMER_RIGHT_17_TM=57.768
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=955
PRIMER_PAIR_18_PENALTY=3.407653
PRIMER_LEFT_18_PENALTY=1.233663
PRIMER_RIGHT_18_PENALTY=2.173990
PRIMER_LEFT_18_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_18_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_18=557,20
PRIMER_RIGHT_18=1507,21
PRIMER_LEFT_18_TM=58.766
PRIMER_RIGHT_18_TM=58.826
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=951
PRIMER_PAIR_19_PENALTY=3.413986
PRIMER_LEFT_19_PENALTY=1.239996
PRIMER_RIGHT_19_PENALTY=2.173990
PRIMER_LEFT_19_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_19_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_19=555,21
PRIMER_RIGHT_19=1507,21
PRIMER_LEFT_19_TM=60.240
PRIMER_RIGHT_19_TM=58.826
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=953
PRIMER_PAIR_20_PENALTY=3.461922
PRIMER_LEFT_20_PENALTY=1.145433
PRIMER_RIGHT_20_PENALTY=2.316489
PRIMER_LEFT_20_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_20_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_20=554,20
PRIMER_RIGHT_20=1509,21
PRIMER_LEFT_20_TM=61.145
PRIMER_RIGHT_20_TM=58.684
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=956
PRIMER_PAIR_21_PENALTY=3.465624
PRIMER_LEFT_21_PENALTY=1.233663
PRIMER_RIGHT_21_PENALTY=2.231961
PRIMER_LEFT_21_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_21_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_21=557,20
PRIMER_RIGHT_21=1506,20
PRIMER_LEFT_21_TM=58.766
PRIMER_RIGHT_21_TM=57.768
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=950
PRIMER_PAIR_22_PENALTY=3.465624
PRIMER_LEFT_22_PENALTY=1.233663
PRIMER_RIGHT_22_PENALTY=2.231961
PRIMER_LEFT_22_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_22_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_22=557,20
PRIMER_RIGHT_22=1507,20
PRIMER_LEFT_22_TM=58.766
PRIMER_RIGHT_22_TM=57.768
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=951
PRIMER_PAIR_23_PENALTY=3.465624
PRIMER_LEFT_23_PENALTY=1.233663
PRIMER_RIGHT_23_PENALTY=2.231961
PRIMER_LEFT_23_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_23_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_23=557,20
PRIMER_RIGHT_23=1508,20
PRIMER_LEFT_23_TM=58.766
PRIMER_RIGHT_23_TM=57.768
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=952
PRIMER_PAIR_24_PENALTY=3.471957
PRIMER_LEFT_24_PENALTY=1.239996
PRIMER_RIGHT_24_PENALTY=2.231961
PRIMER_LEFT_24_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_24_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_24=555,21
PRIMER_RIGHT_24=1506,20
PRIMER_LEFT_24_TM=60.240
PRIMER_RIGHT_24_TM=57.768
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=952
PRIMER_PAIR_25_PENALTY=3.471957
PRIMER_LEFT_25_PENALTY=1.239996
PRIMER_RIGHT_25_PENALTY=2.231961
PRIMER_LEFT_25_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_25_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_25=555,21
PRIMER_RIGHT_25=1507,20
PRIMER_LEFT_25_TM=60.240
PRIMER_RIGHT_25_TM=57.768
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=953
PRIMER_PAIR_26_PENALTY=3.471957
PRIMER_LEFT_26_PENALTY=1.239996
PRIMER_RIGHT_26_PENALTY=2.231961
PRIMER_LEFT_26_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_26_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_26=555,21
PRIMER_RIGHT_26=1508,20
PRIMER_LEFT_26_TM=60.240
PRIMER_RIGHT_26_TM=57.768
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=954
PRIMER_PAIR_27_PENALTY=3.492561
PRIMER_LEFT_27_PENALTY=0.736567
PRIMER_RIGHT_27_PENALTY=2.755994
PRIMER_LEFT_27_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_27_SEQUENCE=TGGGATAACACCAACACCTAAA
PRIMER_LEFT_27=556,20
PRIMER_RIGHT_27=1505,22
PRIMER_LEFT_27_TM=59.263
PRIMER_RIGHT_27_TM=59.244
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.3000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=950
PRIMER_PAIR_28_PENALTY=3.492561
PRIMER_LEFT_28_PENALTY=0.736567
PRIMER_RIGHT_28_PENALTY=2.755994
PRIMER_LEFT_28_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_28_SEQUENCE=TTGGGATAACACCAACACCTAA
PRIMER_LEFT_28=556,20
PRIMER_RIGHT_28=1506,22
PRIMER_LEFT_28_TM=59.263
PRIMER_RIGHT_28_TM=59.244
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=7.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=951
PRIMER_PAIR_29_PENALTY=3.492561
PRIMER_LEFT_29_PENALTY=0.736567
PRIMER_RIGHT_29_PENALTY=2.755994
PRIMER_LEFT_29_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_29_SEQUENCE=TGGGATAACACCAACACCTAAA
PRIMER_LEFT_29=555,20
PRIMER_RIGHT_29=1505,22
PRIMER_LEFT_29_TM=59.263
PRIMER_RIGHT_29_TM=59.244
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.3000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=951
PRIMER_PAIR_30_PENALTY=3.492561
PRIMER_LEFT_30_PENALTY=0.736567
PRIMER_RIGHT_30_PENALTY=2.755994
PRIMER_LEFT_30_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_30_SEQUENCE=TTGGGATAACACCAACACCTAA
PRIMER_LEFT_30=555,20
PRIMER_RIGHT_30=1506,22
PRIMER_LEFT_30_TM=59.263
PRIMER_RIGHT_30_TM=59.244
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.5000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=952
PRIMER_PAIR_31_PENALTY=3.537601
PRIMER_LEFT_31_PENALTY=1.145433
PRIMER_RIGHT_31_PENALTY=2.392169
PRIMER_LEFT_31_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_31_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_31=554,20
PRIMER_RIGHT_31=1508,21
PRIMER_LEFT_31_TM=61.145
PRIMER_RIGHT_31_TM=61.392
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=955
PRIMER_PAIR_32_PENALTY=3.550152
PRIMER_LEFT_32_PENALTY=1.233663
PRIMER_RIGHT_32_PENALTY=2.316489
PRIMER_LEFT_32_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_32_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_32=557,20
PRIMER_RIGHT_32=1509,21
PRIMER_LEFT_32_TM=58.766
PRIMER_RIGHT_32_TM=58.684
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=953
PRIMER_PAIR_33_PENALTY=3.554048
PRIMER_LEFT_33_PENALTY=1.380058
PRIMER_RIGHT_33_PENALTY=2.173990
PRIMER_LEFT_33_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_33_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_33=868,20
PRIMER_RIGHT_33=1507,21
PRIMER_LEFT_33_TM=58.620
PRIMER_RIGHT_33_TM=58.826
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=10.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=640
PRIMER_PAIR_34_PENALTY=3.556485
PRIMER_LEFT_34_PENALTY=1.239996
PRIMER_RIGHT_34_PENALTY=2.316489
PRIMER_LEFT_34_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_34_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_34=555,21
PRIMER_RIGHT_34=1509,21
PRIMER_LEFT_34_TM=60.240
PRIMER_RIGHT_34_TM=58.684
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=955
PRIMER_PAIR_35_PENALTY=3.612019
PRIMER_LEFT_35_PENALTY=1.380058
PRIMER_RIGHT_35_PENALTY=2.231961
PRIMER_LEFT_35_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_35_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_35=868,20
PRIMER_RIGHT_35=1506,20
PRIMER_LEFT_35_TM=58.620
PRIMER_RIGHT_35_TM=57.768
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=10.9000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=639
PRIMER_PAIR_36_PENALTY=3.612019
PRIMER_LEFT_36_PENALTY=1.380058
PRIMER_RIGHT_36_PENALTY=2.231961
PRIMER_LEFT_36_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_36_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_36=868,20
PRIMER_RIGHT_36=1507,20
PRIMER_LEFT_36_TM=58.620
PRIMER_RIGHT_36_TM=57.768
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=640
PRIMER_PAIR_37_PENALTY=3.612019
PRIMER_LEFT_37_PENALTY=1.380058
PRIMER_RIGHT_37_PENALTY=2.231961
PRIMER_LEFT_37_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_37_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_37=868,20
PRIMER_RIGHT_37=1508,20
PRIMER_LEFT_37_TM=58.620
PRIMER_RIGHT_37_TM=57.768
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=10.9000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=641
PRIMER_PAIR_38_PENALTY=3.625831
PRIMER_LEFT_38_PENALTY=1.233663
PRIMER_RIGHT_38_PENALTY=2.392169
PRIMER_LEFT_38_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_38_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_38=557,20
PRIMER_RIGHT_38=1508,21
PRIMER_LEFT_38_TM=58.766
PRIMER_RIGHT_38_TM=61.392
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=952
PRIMER_PAIR_39_PENALTY=3.632165
PRIMER_LEFT_39_PENALTY=1.239996
PRIMER_RIGHT_39_PENALTY=2.392169
PRIMER_LEFT_39_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_39_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_39=555,21
PRIMER_RIGHT_39=1508,21
PRIMER_LEFT_39_TM=60.240
PRIMER_RIGHT_39_TM=61.392
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=954
PRIMER_PAIR_40_PENALTY=3.662084
PRIMER_LEFT_40_PENALTY=1.145433
PRIMER_RIGHT_40_PENALTY=2.516652
PRIMER_LEFT_40_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_40_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_40=554,20
PRIMER_RIGHT_40=1510,22
PRIMER_LEFT_40_TM=61.145
PRIMER_RIGHT_40_TM=60.517
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=957
PRIMER_PAIR_41_PENALTY=3.696547
PRIMER_LEFT_41_PENALTY=1.380058
PRIMER_RIGHT_41_PENALTY=2.316489
PRIMER_LEFT_41_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_41_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_41=868,20
PRIMER_RIGHT_41=1509,21
PRIMER_LEFT_41_TM=58.620
PRIMER_RIGHT_41_TM=58.684
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=10.9000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=642
PRIMER_PAIR_42_PENALTY=3.750314
PRIMER_LEFT_42_PENALTY=1.233663
PRIMER_RIGHT_42_PENALTY=2.516652
PRIMER_LEFT_42_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_42_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_42=557,20
PRIMER_RIGHT_42=1510,22
PRIMER_LEFT_42_TM=58.766
PRIMER_RIGHT_42_TM=60.517
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=954
PRIMER_PAIR_43_PENALTY=3.756648
PRIMER_LEFT_43_PENALTY=1.239996
PRIMER_RIGHT_43_PENALTY=2.516652
PRIMER_LEFT_43_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_43_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_43=555,21
PRIMER_RIGHT_43=1510,22
PRIMER_LEFT_43_TM=60.240
PRIMER_RIGHT_43_TM=60.517
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=956
PRIMER_PAIR_44_PENALTY=3.772226
PRIMER_LEFT_44_PENALTY=1.380058
PRIMER_RIGHT_44_PENALTY=2.392169
PRIMER_LEFT_44_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_44_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_44=868,20
PRIMER_RIGHT_44=1508,21
PRIMER_LEFT_44_TM=58.620
PRIMER_RIGHT_44_TM=61.392
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.9000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=641
PRIMER_PAIR_45_PENALTY=3.778313
PRIMER_LEFT_45_PENALTY=1.604323
PRIMER_RIGHT_45_PENALTY=2.173990
PRIMER_LEFT_45_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_45_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_45=557,21
PRIMER_RIGHT_45=1507,21
PRIMER_LEFT_45_TM=60.604
PRIMER_RIGHT_45_TM=58.826
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=951
PRIMER_PAIR_46_PENALTY=3.778313
PRIMER_LEFT_46_PENALTY=1.604323
PRIMER_RIGHT_46_PENALTY=2.173990
PRIMER_LEFT_46_SEQUENCE=TCCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_46_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_46=556,21
PRIMER_RIGHT_46=1507,21
PRIMER_LEFT_46_TM=60.604
PRIMER_RIGHT_46_TM=58.826
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=952
PRIMER_PAIR_47_PENALTY=3.836284
PRIMER_LEFT_47_PENALTY=1.604323
PRIMER_RIGHT_47_PENALTY=2.231961
PRIMER_LEFT_47_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_47_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_47=557,21
PRIMER_RIGHT_47=1506,20
PRIMER_LEFT_47_TM=60.604
PRIMER_RIGHT_47_TM=57.768
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=950
PRIMER_PAIR_48_PENALTY=3.836284
PRIMER_LEFT_48_PENALTY=1.604323
PRIMER_RIGHT_48_PENALTY=2.231961
PRIMER_LEFT_48_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_48_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_48=557,21
PRIMER_RIGHT_48=1507,20
PRIMER_LEFT_48_TM=60.604
PRIMER_RIGHT_48_TM=57.768
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=951
PRIMER_PAIR_49_PENALTY=3.836284
PRIMER_LEFT_49_PENALTY=1.604323
PRIMER_RIGHT_49_PENALTY=2.231961
PRIMER_LEFT_49_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_49_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_49=557,21
PRIMER_RIGHT_49=1508,20
PRIMER_LEFT_49_TM=60.604
PRIMER_RIGHT_49_TM=57.768
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=952
=
SEQUENCE_ID=MAGIC.433_1_13544600_13544814_ct-1_wil-2_341963_43419_TRUE
SEQUENCE_TARGET=950,314
SEQUENCE_TEMPLATE=AATCTTCTAAACANGGTATAAAACNAAACCNTCCAGAAAAACAGAGGTTTTGGTCCTCTAGTATTCTTATCTGTATNGCTCGGTTTTTAAAGGCCTAAGTAAATATTTGTGATGCAGGTGGAGCCGTCGAAAAGAGAAGAGCTGGTGGCTGCTATTCGAAAGGTCTTGGGTTGACTGATATCGAAGACTTTAGCTTCTAATCCAAGAAAGATGGACATTTAAAGTTTGCTTGTGTCCATTTGGACCATCTTCCTTATATTTGTTGGTCACAGTTGTAAATGTTGTTGTAGCTTTGTCATTTCCGTATAAACAAATTACGCAATAATTCATTCAACATGTCACTCTTGCTTCATATTTATACACTGAACCAAGACAATATAATAGTCTAAATATAAAACTGNTCTTGCTTAACCAAATACTGTAAAGCTAAGAATNNCAAGANAANTTATATAGTCTAACTGAAANTAAACAATGATTAGGCCANNACAAATAAANTGAACCGANTGTATNGAANNGAAACNNNAGNNAAAANNNNGAACCNAANTAAAAATTGGAAAATAANNGAATGCATACTAAATTTTATAACNNAAACCNAAAANATACNTACCAANNNNNNTTGGGTAACCAAAAATNTNCAAAATATAAATATATTTTNAAANATATTAGTTATTTCTAGATTAAATAATTAAAATTAGTTNAAAATATATANAATANTAAAAATACTNNNNNNNNNNCGAAAATATCCAAAAATATTTAAAGAAAAATAACNGAATGNATACTAAATTTTAAAACCAAANGAATCGGAACCGAACCNAAACCGANCCGNACTTTCAAAATAACCNAATGGATACTAAACTTTAGAACCGAAAAAANNGATAACTAAATAAATATATNCCGAAACCGAATATATAATCGAATGCCCANGACTAACAATGACAATAATNAAAAAATTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNAAAAGACATCCTAGTGAATAAANNTTTTTTCTCTTTTGTGCAGTGTNCTCCTTTCATCTNTCTCTCNCNTTTNTTACGAATCTTTACATACATCACCATCAACTGAACAAAANTCAAAATTATATATNNNTCAATCTTAAATGTNGATNNGATCACCNAAATCTGATTTACTCTATTTTATAACAACACNATTAGCTANAAANCCAACCATTAGNGCAACAATACTCTNACTATATTTATTCTTNGTNAGCCAGANAATACTAGNAAAATCNGGTGAAATCNTTTCGNCAATTATGTTAAATTAATCTTTANTTTNCCATCAAAGTTNATATGNGAATTTTAATTTTTAAATCNATGNAAAATAAAATTGAGAATTNTGAACNANCAAATTGACGATCATGTTANGGATTTCTTAGATCTAGAAGCGATAAAGGGTTTANGATGGTGGNAATTNTAAAGNGNAATAGCCAATAAATCGATTTTGATTTTGGAAGATTTTTGAGAGATANTATTCTATTTTGTGAATATATTATTTATGTATACCTTGTACNATNTACGTTTTAAAATGANTTGTTTGTTTTGATTAAGGATGTNAGTGNNNNNNNNATTNTTTTAGATTAAAATCCTTGGGTTCCNTATNTATTATCTTAGGNTTTAGTATCNTTCCNTNAAAAANTATTTAATTNATTATTTGGATTCATCNGAATCAAAGTTTTGGTTTGANCAAATATATATTATTNTATTTTTCTATGATGATATGTATAGAATTTGGTATCACTATTCATTTTTTAANAAATTCNTATCCGATTTTTAAAATTCAAATCNNNNNNNNTANANAAAAAAATCTGTTTTTTTAATANTATTATTANCTTTAACNAANNNNNNNNNNNNNNNTAATATANTAAACAATATTAAANGGGNNGNACATANTTTAATCATATTTCTNTNTCCTCTTCTCNACATAACTCAAANTTNTCTTCCTCCTNAAAANGCNACNTCAGCATAATTNACTNCGCTTCTGTTTTCTTCANGCGCCTTAANGCNGAATGAAAGANNNGGGTGAAGGAGTNTNTTGTCATTACACANCNCACTTNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_5924387_5924907_po-0_bur-0_3759_4770_TRUE
SEQUENCE_TARGET=950,620
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTATTTACNTTNTCCNAGANCAATCAAAATGNNTNNTTTTTCTATAAGATGAAANTTATAGAGNTTNCACNTCGGCAANNNTAGATAATTTCATCAATATTCATTACACTCATATANANTNTTATNTNNNNNNNTTGNTNGTCTCATAATGTGNGGCTTGTGATGTTTGNTTCAATACCTTGTCNCAGATTAAACNTCAGACGAGNNNTNAGGGATNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCATAACTNATTTTCTNAAAATATTTTGAAANTNACATACGGATGATCTCNTNAATTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCAAATTNNGATCCAAACCGGANAANNNTGTATTGTNNNANTTTAAAATNTCCAAACNGGCTTTCCTTCGNTTNNCNGTTAAANNATCTAATTTTNGNTTTNAAANTATAATTTNAGAACTCNNACGTTTTNTNNNNNNNNNNNTATGTNTTGNTCCTCGAGTTTACAATCNTCGNTTCNATAATCNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNGNATANNTATTAAATNNTNTTCTAAAAAGCGATTTANNNNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCNTAANATCNTATATANNTCGNTTTANTATATNTTTNTANTTTTNAANNAATTTTATATTTNTATAAATAGTTATAAATGTTNATATAGTANNTNATATAGTTAANNNAATATTTTGTATANNTTNNNTTTANGATTNNACNNNNNNNNATATANNNNNNNNNNNNNTTAANNNNNATTTTATNTANTNTTTANGTANAAAATNATATTTATANNNTNTNTATTATAANNGCTNNNNNNNNTAANATNATNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATTNGATCACNGNNCGTTTAACGNCTANCGNTTTCTTNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNAACTANANNAAATATCCAANAANTTTCCAANNNTNGTGGAACCTACACGATATGGTTCACATATTTTCCATGAAAGGATAACTATTTTGACTTTTCTCATCACGCTCAATAATAGCGTGTTGTTAATCCTTTACTCTATCTCTCTTNAAANNTCNNTTGATCTTANATCNTCTCTNNATTGTAGTAATGGANNTTTACGTTTTNCTTGGGACTNTTGNTGCTACGNTCTTNNNTCTTTTGNGTACAANATTTTNTATGANTTNTNGAAAANTTNGAGTCCAAGAAANTATANTAACTACTNCTTCTTCACCTCCTACATCTNCCCAATCNTCTTTGTCTTGNNACCAGAAACACGATGTCTTCCCCAGCTTCCACGGNGCAGATGTGCGCAAANCATTTCTCAGTCACATTTTGAAAGAGTTTANAAGAAAAGGGATNGATACNTTCATTGATAATNATATNGAGAGNAGCAAATCNATCNGTCNAGAACTCNTNGAAGCNATCANAGGATCTAAGATTGCNGTTGTCTTGCTCTCGAAGGACTACGCTTCCTCGTCNTGGTGCNTNAACGAGTTGGTNGAGATCATGAAGTGTAGGAAAATGTTGGATCAAACAGTGATGACNATTTTCTATNAAGTNNANCCAACNGATGTNAAGAAGCAAACNNGANANTTTGGGAANGTCTTCAAGAAAACTTGTNTNGGTAAAACAAATNNAGTTANTNGNAAATGGATNNAGGCTTTGAGTGAAGTAGCCACAATAGCTGGAGAGCATTCAANNAATTGGTTAGTTCTTTTTANCACATNNNNCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_11585085_11585202_hi-0_ct-1_260155_763141_TRUE
SEQUENCE_TARGET=950,217
SEQUENCE_TEMPLATE=GACTTNGCAACAGGATCTCAAAGTTCTTNNNAATAAGAAAAAAANATATTAACCGTGACGTTATTGANNNTTTNTTTCTTTCTTNNNTCTAGTTTTGTGTTGTGCTGTATTTTNTTTCTTTTGTTATGTTCTGNTTTTNTGTTACCTTTGAGTGTGTTTGGAGGNTTTNTTGCGTGAGATATGATTTGTTTTTGGTAGTGTTTGANNTTTGAGTGTTTATGTTTGTGTTTTGAGTTTGAGTGCATTGTGTNTGAGTGATNNNCGTGTCTGTTTCTAGATAACGTGTGTTTTCCGATAGTGTGAGTAACCNAAACAAGCCAAGACTCAGTTCTTCAAATACAAATAACCTGTCGAGATGACACAANAAACATGAGANTANNGTGAGAGATATCTGANCTGTCATATGCTCAAAGATATCAGCATGAGNCAAGAAACACGNCCCTTACTTGNACTTACTACTAAGCCAAAATTTCACCTGAAATACANNACATAAACCATAAAATGAAACCACAAAATCAATGCCAAGAAGAAGAAANACTTGGTAAGAGGTGTTTATATAGCCAAAATCTGACCGCTTGCTAANTTTATTACTTTCAATCTTGATGTGTATGACNAAGATCCAAGCTGAAAAACATAACTATCNNAAACAATCTCACGCATGAGGAACAAAANGGCAGANAACACATTAAGATNANAAAANGAGACTCCAATGCATCATCTAGGCAACAAACATAAATTGAAACAGTGATCGACTGATGATGAAACAATTTAAACTCATNTACTTGAACAAATAACAGAGAAGTTTGNTGAAACANTTCCATAACATTAAANTCTTATGNNNNNNNNNNNNAAATCCATNTACGCGATTATCCTAATTGGTAGATCTCATAACCATCNAAAACAACTAAATTCCAACAGAAATCAAGCAAACAATTTCAAAACTAAAACCCTAAATNCCAAATCAACATACACACTGATCGAAATCTTGCGCANCTTCTTCNATTTGGTANTACTATCACACGATCCAANTNCAATCNGNGTNTCCATCGTTGGATTNTCACCGANCCAAAATCGAAACCCTTGGATNCCGATTTGAACTANCCCAACGAATCAGANGNCATTGAANANGANTCANTTNATCGGAGAAAAGANTCAGANGAAGATANACGAACAAAATCNCTTCTCCACTCAAATTCANACTCGNTTTGAAGAAATTANAAATGAATTTNNAAATTTGAATATTCGNNGACGGTTTATNTGTNGAAGAAGAGTTTACGTTGCGNAAATCAGCTATTTATNTGANTCTTCGANACAAAGANCACGTAGATTTACGNNNATGCCCTTNTNCGTATATGCTCGAACNGNNTTATTTTCAAAATTGTGTTTCCCGCTTTGGAGATATTTAGTTAGAATCCCATTAAAAACTTTGGAGATAATAGGTTCAATTTGTTCNNAAAAACTAGNGTTGAAGATGAACCCTTCNTTTAATTAGTAGCAAATTATCATCTGCTTGCTCTNNTTTTTTTTNNCCATTTAATTAGTAAATATACAANNNTTGTTTATTTAGTTTNCNTTNTTTTTACATANAATTATATATTTNNAATATNNATTAGNGAAANTTTANNNNAAAANNATTTTGAAAANTCAAATTAANNANANAGAGAAATAAAGCTCATGAATCTCTCTTGTTCANAACANAAAAAAGAAAGNTTGGTTGANAAAGAAACCAAATCGTTGAACNCTATTTAAGTGAACGANCTNCCCAAAAGAAGNCAGNAGGNCGGCTNTCTATAGTNTATANCNGAGGANTCTTCTGATCGCATATAANAANGATTNAGGAGTTGCAAAANGTAAATNAAATCNTTACACTGATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNGACCCCNAAAACANAGCTCTCTGNNNANANATGGTGAGATTTCAAATTATGCTTTTNAAGNGAAAAGNTTTNGGGCAGCCAAGAAAAGCNTTGTACGATGAGTATCATGAGATCTTNTCAAGTCATCTTCNAGATTTGAATAGNNNCTTCTTCTTCNCCTCTTGNNNNNNCTTAACAACTTGAGGAGAAGATTTGGTTCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.929780
PRIMER_LEFT_0_PENALTY=0.353748
PRIMER_RIGHT_0_PENALTY=0.576032
PRIMER_LEFT_0_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_0_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_0=706,20
PRIMER_RIGHT_0=1400,20
PRIMER_LEFT_0_TM=59.646
PRIMER_RIGHT_0_TM=60.576
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=695
PRIMER_PAIR_1_PENALTY=1.057988
PRIMER_LEFT_1_PENALTY=0.481956
PRIMER_RIGHT_1_PENALTY=0.576032
PRIMER_LEFT_1_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_1_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_1=507,20
PRIMER_RIGHT_1=1400,20
PRIMER_LEFT_1_TM=59.518
PRIMER_RIGHT_1_TM=60.576
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=894
PRIMER_PAIR_2_PENALTY=1.706782
PRIMER_LEFT_2_PENALTY=0.353748
PRIMER_RIGHT_2_PENALTY=1.353034
PRIMER_LEFT_2_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_2_SEQUENCE=AATATCTCCAAAGCGGGAAA
PRIMER_LEFT_2=706,20
PRIMER_RIGHT_2=1402,20
PRIMER_LEFT_2_TM=59.646
PRIMER_RIGHT_2_TM=58.647
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=697
PRIMER_PAIR_3_PENALTY=1.706782
PRIMER_LEFT_3_PENALTY=0.353748
PRIMER_RIGHT_3_PENALTY=1.353034
PRIMER_LEFT_3_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_3_SEQUENCE=AAATATCTCCAAAGCGGGAA
PRIMER_LEFT_3=706,20
PRIMER_RIGHT_3=1403,20
PRIMER_LEFT_3_TM=59.646
PRIMER_RIGHT_3_TM=58.647
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=698
PRIMER_PAIR_4_PENALTY=1.736416
PRIMER_LEFT_4_PENALTY=0.353748
PRIMER_RIGHT_4_PENALTY=1.382668
PRIMER_LEFT_4_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_4_SEQUENCE=ATCTCCAAAGCGGGAAACAC
PRIMER_LEFT_4=706,20
PRIMER_RIGHT_4=1399,20
PRIMER_LEFT_4_TM=59.646
PRIMER_RIGHT_4_TM=61.383
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=694
PRIMER_PAIR_5_PENALTY=1.834990
PRIMER_LEFT_5_PENALTY=0.481956
PRIMER_RIGHT_5_PENALTY=1.353034
PRIMER_LEFT_5_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_5_SEQUENCE=AATATCTCCAAAGCGGGAAA
PRIMER_LEFT_5=507,20
PRIMER_RIGHT_5=1402,20
PRIMER_LEFT_5_TM=59.518
PRIMER_RIGHT_5_TM=58.647
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=896
PRIMER_PAIR_6_PENALTY=1.834990
PRIMER_LEFT_6_PENALTY=0.481956
PRIMER_RIGHT_6_PENALTY=1.353034
PRIMER_LEFT_6_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_6_SEQUENCE=AAATATCTCCAAAGCGGGAA
PRIMER_LEFT_6=507,20
PRIMER_RIGHT_6=1403,20
PRIMER_LEFT_6_TM=59.518
PRIMER_RIGHT_6_TM=58.647
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=897
PRIMER_PAIR_7_PENALTY=1.864624
PRIMER_LEFT_7_PENALTY=0.481956
PRIMER_RIGHT_7_PENALTY=1.382668
PRIMER_LEFT_7_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_7_SEQUENCE=ATCTCCAAAGCGGGAAACAC
PRIMER_LEFT_7=507,20
PRIMER_RIGHT_7=1399,20
PRIMER_LEFT_7_TM=59.518
PRIMER_RIGHT_7_TM=61.383
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=893
PRIMER_PAIR_8_PENALTY=1.873125
PRIMER_LEFT_8_PENALTY=1.297092
PRIMER_RIGHT_8_PENALTY=0.576032
PRIMER_LEFT_8_SEQUENCE=CAATGCATCATCTAGGCAACA
PRIMER_RIGHT_8_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_8=707,21
PRIMER_RIGHT_8=1400,20
PRIMER_LEFT_8_TM=59.703
PRIMER_RIGHT_8_TM=60.576
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=2.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=694
PRIMER_PAIR_9_PENALTY=1.899096
PRIMER_LEFT_9_PENALTY=0.353748
PRIMER_RIGHT_9_PENALTY=1.545348
PRIMER_LEFT_9_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_9_SEQUENCE=AATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_9=706,20
PRIMER_RIGHT_9=1402,21
PRIMER_LEFT_9_TM=59.646
PRIMER_RIGHT_9_TM=59.455
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=10.0000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=2.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=697
PRIMER_PAIR_10_PENALTY=2.027304
PRIMER_LEFT_10_PENALTY=0.481956
PRIMER_RIGHT_10_PENALTY=1.545348
PRIMER_LEFT_10_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_10_SEQUENCE=AATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_10=507,20
PRIMER_RIGHT_10=1402,21
PRIMER_LEFT_10_TM=59.518
PRIMER_RIGHT_10_TM=59.455
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=896
PRIMER_PAIR_11_PENALTY=2.059846
PRIMER_LEFT_11_PENALTY=1.483814
PRIMER_RIGHT_11_PENALTY=0.576032
PRIMER_LEFT_11_SEQUENCE=CCAATGCATCATCTAGGCAAC
PRIMER_RIGHT_11_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_11=706,21
PRIMER_RIGHT_11=1400,20
PRIMER_LEFT_11_TM=60.484
PRIMER_RIGHT_11_TM=60.576
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=695
PRIMER_PAIR_12_PENALTY=2.100387
PRIMER_LEFT_12_PENALTY=1.524354
PRIMER_RIGHT_12_PENALTY=0.576032
PRIMER_LEFT_12_SEQUENCE=ACCACAAAATCAATGCCAAG
PRIMER_RIGHT_12_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_12=506,20
PRIMER_RIGHT_12=1400,20
PRIMER_LEFT_12_TM=58.476
PRIMER_RIGHT_12_TM=60.576
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=2.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=895
PRIMER_PAIR_13_PENALTY=2.160488
PRIMER_LEFT_13_PENALTY=0.353748
PRIMER_RIGHT_13_PENALTY=1.806740
PRIMER_LEFT_13_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_13_SEQUENCE=ATATCTCCAAAGCGGGAAACA
PRIMER_LEFT_13=706,20
PRIMER_RIGHT_13=1401,21
PRIMER_LEFT_13_TM=59.646
PRIMER_RIGHT_13_TM=60.807
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=696
PRIMER_PAIR_14_PENALTY=2.218076
PRIMER_LEFT_14_PENALTY=0.353748
PRIMER_RIGHT_14_PENALTY=1.864328
PRIMER_LEFT_14_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_14_SEQUENCE=ATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_14=706,20
PRIMER_RIGHT_14=1401,20
PRIMER_LEFT_14_TM=59.646
PRIMER_RIGHT_14_TM=58.136
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=10.0000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=696
PRIMER_PAIR_15_PENALTY=2.288696
PRIMER_LEFT_15_PENALTY=0.481956
PRIMER_RIGHT_15_PENALTY=1.806740
PRIMER_LEFT_15_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_15_SEQUENCE=ATATCTCCAAAGCGGGAAACA
PRIMER_LEFT_15=507,20
PRIMER_RIGHT_15=1401,21
PRIMER_LEFT_15_TM=59.518
PRIMER_RIGHT_15_TM=60.807
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=895
PRIMER_PAIR_16_PENALTY=2.346284
PRIMER_LEFT_16_PENALTY=0.481956
PRIMER_RIGHT_16_PENALTY=1.864328
PRIMER_LEFT_16_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_16_SEQUENCE=ATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_16=507,20
PRIMER_RIGHT_16=1401,20
PRIMER_LEFT_16_TM=59.518
PRIMER_RIGHT_16_TM=58.136
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=895
PRIMER_PAIR_17_PENALTY=2.650126
PRIMER_LEFT_17_PENALTY=1.297092
PRIMER_RIGHT_17_PENALTY=1.353034
PRIMER_LEFT_17_SEQUENCE=CAATGCATCATCTAGGCAACA
PRIMER_RIGHT_17_SEQUENCE=AATATCTCCAAAGCGGGAAA
PRIMER_LEFT_17=707,21
PRIMER_RIGHT_17=1402,20
PRIMER_LEFT_17_TM=59.703
PRIMER_RIGHT_17_TM=58.647
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=2.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=696
PRIMER_PAIR_18_PENALTY=2.650126
PRIMER_LEFT_18_PENALTY=1.297092
PRIMER_RIGHT_18_PENALTY=1.353034
PRIMER_LEFT_18_SEQUENCE=CAATGCATCATCTAGGCAACA
PRIMER_RIGHT_18_SEQUENCE=AAATATCTCCAAAGCGGGAA
PRIMER_LEFT_18=707,21
PRIMER_RIGHT_18=1403,20
PRIMER_LEFT_18_TM=59.703
PRIMER_RIGHT_18_TM=58.647
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=2.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=697
PRIMER_PAIR_19_PENALTY=2.661326
PRIMER_LEFT_19_PENALTY=2.085294
PRIMER_RIGHT_19_PENALTY=0.576032
PRIMER_LEFT_19_SEQUENCE=GAAACCACAAAATCAATGCC
PRIMER_RIGHT_19_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_19=503,20
PRIMER_RIGHT_19=1400,20
PRIMER_LEFT_19_TM=57.915
PRIMER_RIGHT_19_TM=60.576
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=2.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=898
PRIMER_PAIR_20_PENALTY=2.679760
PRIMER_LEFT_20_PENALTY=1.297092
PRIMER_RIGHT_20_PENALTY=1.382668
PRIMER_LEFT_20_SEQUENCE=CAATGCATCATCTAGGCAACA
PRIMER_RIGHT_20_SEQUENCE=ATCTCCAAAGCGGGAAACAC
PRIMER_LEFT_20=707,21
PRIMER_RIGHT_20=1399,20
PRIMER_LEFT_20_TM=59.703
PRIMER_RIGHT_20_TM=61.383
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=693
PRIMER_PAIR_21_PENALTY=2.696045
PRIMER_LEFT_21_PENALTY=0.353748
PRIMER_RIGHT_21_PENALTY=2.342297
PRIMER_LEFT_21_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_21_SEQUENCE=TATCTCCAAAGCGGGAAACAC
PRIMER_LEFT_21=706,20
PRIMER_RIGHT_21=1400,21
PRIMER_LEFT_21_TM=59.646
PRIMER_RIGHT_21_TM=61.342
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=10.0000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=2.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=695
PRIMER_PAIR_22_PENALTY=2.715956
PRIMER_LEFT_22_PENALTY=2.139923
PRIMER_RIGHT_22_PENALTY=0.576032
PRIMER_LEFT_22_SEQUENCE=TGCATCATCTAGGCAACAAA
PRIMER_RIGHT_22_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_22=710,20
PRIMER_RIGHT_22=1400,20
PRIMER_LEFT_22_TM=57.860
PRIMER_RIGHT_22_TM=60.576
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=691
PRIMER_PAIR_23_PENALTY=2.750127
PRIMER_LEFT_23_PENALTY=0.353748
PRIMER_RIGHT_23_PENALTY=2.396379
PRIMER_LEFT_23_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_23_SEQUENCE=CTAAATATCTCCAAAGCGGGAA
PRIMER_LEFT_23=706,20
PRIMER_RIGHT_23=1405,22
PRIMER_LEFT_23_TM=59.646
PRIMER_RIGHT_23_TM=59.604
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.0000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=700
PRIMER_PAIR_24_PENALTY=2.788894
PRIMER_LEFT_24_PENALTY=2.212862
PRIMER_RIGHT_24_PENALTY=0.576032
PRIMER_LEFT_24_SEQUENCE=GCATCATCTAGGCAACAAACA
PRIMER_RIGHT_24_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_24=711,21
PRIMER_RIGHT_24=1400,20
PRIMER_LEFT_24_TM=58.787
PRIMER_RIGHT_24_TM=60.576
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=690
PRIMER_PAIR_25_PENALTY=2.788894
PRIMER_LEFT_25_PENALTY=2.212862
PRIMER_RIGHT_25_PENALTY=0.576032
PRIMER_LEFT_25_SEQUENCE=TGCATCATCTAGGCAACAAAC
PRIMER_RIGHT_25_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_25=710,21
PRIMER_RIGHT_25=1400,20
PRIMER_LEFT_25_TM=58.787
PRIMER_RIGHT_25_TM=60.576
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=691
PRIMER_PAIR_26_PENALTY=2.824253
PRIMER_LEFT_26_PENALTY=0.481956
PRIMER_RIGHT_26_PENALTY=2.342297
PRIMER_LEFT_26_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_26_SEQUENCE=TATCTCCAAAGCGGGAAACAC
PRIMER_LEFT_26=507,20
PRIMER_RIGHT_26=1400,21
PRIMER_LEFT_26_TM=59.518
PRIMER_RIGHT_26_TM=61.342
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=894
PRIMER_PAIR_27_PENALTY=2.836848
PRIMER_LEFT_27_PENALTY=1.483814
PRIMER_RIGHT_27_PENALTY=1.353034
PRIMER_LEFT_27_SEQUENCE=CCAATGCATCATCTAGGCAAC
PRIMER_RIGHT_27_SEQUENCE=AATATCTCCAAAGCGGGAAA
PRIMER_LEFT_27=706,21
PRIMER_RIGHT_27=1402,20
PRIMER_LEFT_27_TM=60.484
PRIMER_RIGHT_27_TM=58.647
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=697
PRIMER_PAIR_28_PENALTY=2.836848
PRIMER_LEFT_28_PENALTY=1.483814
PRIMER_RIGHT_28_PENALTY=1.353034
PRIMER_LEFT_28_SEQUENCE=CCAATGCATCATCTAGGCAAC
PRIMER_RIGHT_28_SEQUENCE=AAATATCTCCAAAGCGGGAA
PRIMER_LEFT_28=706,21
PRIMER_RIGHT_28=1403,20
PRIMER_LEFT_28_TM=60.484
PRIMER_RIGHT_28_TM=58.647
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=2.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=698
PRIMER_PAIR_29_PENALTY=2.842440
PRIMER_LEFT_29_PENALTY=1.297092
PRIMER_RIGHT_29_PENALTY=1.545348
PRIMER_LEFT_29_SEQUENCE=CAATGCATCATCTAGGCAACA
PRIMER_RIGHT_29_SEQUENCE=AATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_29=707,21
PRIMER_RIGHT_29=1402,21
PRIMER_LEFT_29_TM=59.703
PRIMER_RIGHT_29_TM=59.455
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=2.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=696
PRIMER_PAIR_30_PENALTY=2.866482
PRIMER_LEFT_30_PENALTY=1.483814
PRIMER_RIGHT_30_PENALTY=1.382668
PRIMER_LEFT_30_SEQUENCE=CCAATGCATCATCTAGGCAAC
PRIMER_RIGHT_30_SEQUENCE=ATCTCCAAAGCGGGAAACAC
PRIMER_LEFT_30=706,21
PRIMER_RIGHT_30=1399,20
PRIMER_LEFT_30_TM=60.484
PRIMER_RIGHT_30_TM=61.383
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=2.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=694
PRIMER_PAIR_31_PENALTY=2.877388
PRIMER_LEFT_31_PENALTY=1.524354
PRIMER_RIGHT_31_PENALTY=1.353034
PRIMER_LEFT_31_SEQUENCE=ACCACAAAATCAATGCCAAG
PRIMER_RIGHT_31_SEQUENCE=AATATCTCCAAAGCGGGAAA
PRIMER_LEFT_31=506,20
PRIMER_RIGHT_31=1402,20
PRIMER_LEFT_31_TM=58.476
PRIMER_RIGHT_31_TM=58.647
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=897
PRIMER_PAIR_32_PENALTY=2.877388
PRIMER_LEFT_32_PENALTY=1.524354
PRIMER_RIGHT_32_PENALTY=1.353034
PRIMER_LEFT_32_SEQUENCE=ACCACAAAATCAATGCCAAG
PRIMER_RIGHT_32_SEQUENCE=AAATATCTCCAAAGCGGGAA
PRIMER_LEFT_32=506,20
PRIMER_RIGHT_32=1403,20
PRIMER_LEFT_32_TM=58.476
PRIMER_RIGHT_32_TM=58.647
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=9.7000
PRIMER_PAIR_32_COMPL_ANY=2.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=898
PRIMER_PAIR_33_PENALTY=2.878335
PRIMER_LEFT_33_PENALTY=0.481956
PRIMER_RIGHT_33_PENALTY=2.396379
PRIMER_LEFT_33_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_33_SEQUENCE=CTAAATATCTCCAAAGCGGGAA
PRIMER_LEFT_33=507,20
PRIMER_RIGHT_33=1405,22
PRIMER_LEFT_33_TM=59.518
PRIMER_RIGHT_33_TM=59.604
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=40.909
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=899
PRIMER_PAIR_34_PENALTY=2.907022
PRIMER_LEFT_34_PENALTY=1.524354
PRIMER_RIGHT_34_PENALTY=1.382668
PRIMER_LEFT_34_SEQUENCE=ACCACAAAATCAATGCCAAG
PRIMER_RIGHT_34_SEQUENCE=ATCTCCAAAGCGGGAAACAC
PRIMER_LEFT_34=506,20
PRIMER_RIGHT_34=1399,20
PRIMER_LEFT_34_TM=58.476
PRIMER_RIGHT_34_TM=61.383
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=2.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=894
PRIMER_PAIR_35_PENALTY=3.001328
PRIMER_LEFT_35_PENALTY=0.353748
PRIMER_RIGHT_35_PENALTY=2.647580
PRIMER_LEFT_35_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_35_SEQUENCE=AAATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_35=706,20
PRIMER_RIGHT_35=1403,22
PRIMER_LEFT_35_TM=59.646
PRIMER_RIGHT_35_TM=60.648
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=10.0000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=698
PRIMER_PAIR_36_PENALTY=3.004888
PRIMER_LEFT_36_PENALTY=0.353748
PRIMER_RIGHT_36_PENALTY=2.651140
PRIMER_LEFT_36_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_36_SEQUENCE=CTAAATATCTCCAAAGCGGGA
PRIMER_LEFT_36=706,20
PRIMER_RIGHT_36=1405,21
PRIMER_LEFT_36_TM=59.646
PRIMER_RIGHT_36_TM=58.349
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.0000
PRIMER_RIGHT_36_END_STABILITY=11.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=700
PRIMER_PAIR_37_PENALTY=3.008734
PRIMER_LEFT_37_PENALTY=0.353748
PRIMER_RIGHT_37_PENALTY=2.654986
PRIMER_LEFT_37_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_37_SEQUENCE=TAAATATCTCCAAAGCGGGA
PRIMER_LEFT_37=706,20
PRIMER_RIGHT_37=1404,20
PRIMER_LEFT_37_TM=59.646
PRIMER_RIGHT_37_TM=57.345
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=10.0000
PRIMER_RIGHT_37_END_STABILITY=11.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=699
PRIMER_PAIR_38_PENALTY=3.029162
PRIMER_LEFT_38_PENALTY=1.483814
PRIMER_RIGHT_38_PENALTY=1.545348
PRIMER_LEFT_38_SEQUENCE=CCAATGCATCATCTAGGCAAC
PRIMER_RIGHT_38_SEQUENCE=AATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_38=706,21
PRIMER_RIGHT_38=1402,21
PRIMER_LEFT_38_TM=60.484
PRIMER_RIGHT_38_TM=59.455
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=2.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=697
PRIMER_PAIR_39_PENALTY=3.069702
PRIMER_LEFT_39_PENALTY=1.524354
PRIMER_RIGHT_39_PENALTY=1.545348
PRIMER_LEFT_39_SEQUENCE=ACCACAAAATCAATGCCAAG
PRIMER_RIGHT_39_SEQUENCE=AATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_39=506,20
PRIMER_RIGHT_39=1402,21
PRIMER_LEFT_39_TM=58.476
PRIMER_RIGHT_39_TM=59.455
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=2.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=897
PRIMER_PAIR_40_PENALTY=3.089883
PRIMER_LEFT_40_PENALTY=2.513851
PRIMER_RIGHT_40_PENALTY=0.576032
PRIMER_LEFT_40_SEQUENCE=TCCAATGCATCATCTAGGCAA
PRIMER_RIGHT_40_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_40=705,21
PRIMER_RIGHT_40=1400,20
PRIMER_LEFT_40_TM=61.514
PRIMER_RIGHT_40_TM=60.576
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=10.0000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=696
PRIMER_PAIR_41_PENALTY=3.103833
PRIMER_LEFT_41_PENALTY=1.297092
PRIMER_RIGHT_41_PENALTY=1.806740
PRIMER_LEFT_41_SEQUENCE=CAATGCATCATCTAGGCAACA
PRIMER_RIGHT_41_SEQUENCE=ATATCTCCAAAGCGGGAAACA
PRIMER_LEFT_41=707,21
PRIMER_RIGHT_41=1401,21
PRIMER_LEFT_41_TM=59.703
PRIMER_RIGHT_41_TM=60.807
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=695
PRIMER_PAIR_42_PENALTY=3.129536
PRIMER_LEFT_42_PENALTY=0.481956
PRIMER_RIGHT_42_PENALTY=2.647580
PRIMER_LEFT_42_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_42_SEQUENCE=AAATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_42=507,20
PRIMER_RIGHT_42=1403,22
PRIMER_LEFT_42_TM=59.518
PRIMER_RIGHT_42_TM=60.648
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=897
PRIMER_PAIR_43_PENALTY=3.133096
PRIMER_LEFT_43_PENALTY=0.481956
PRIMER_RIGHT_43_PENALTY=2.651140
PRIMER_LEFT_43_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_43_SEQUENCE=CTAAATATCTCCAAAGCGGGA
PRIMER_LEFT_43=507,20
PRIMER_RIGHT_43=1405,21
PRIMER_LEFT_43_TM=59.518
PRIMER_RIGHT_43_TM=58.349
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=11.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=899
PRIMER_PAIR_44_PENALTY=3.136942
PRIMER_LEFT_44_PENALTY=0.481956
PRIMER_RIGHT_44_PENALTY=2.654986
PRIMER_LEFT_44_SEQUENCE=CCACAAAATCAATGCCAAGA
PRIMER_RIGHT_44_SEQUENCE=TAAATATCTCCAAAGCGGGA
PRIMER_LEFT_44=507,20
PRIMER_RIGHT_44=1404,20
PRIMER_LEFT_44_TM=59.518
PRIMER_RIGHT_44_TM=57.345
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=11.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=898
PRIMER_PAIR_45_PENALTY=3.148895
PRIMER_LEFT_45_PENALTY=2.572863
PRIMER_RIGHT_45_PENALTY=0.576032
PRIMER_LEFT_45_SEQUENCE=CCAAAATCTGACCGCTTGCT
PRIMER_RIGHT_45_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_45=560,20
PRIMER_RIGHT_45=1400,20
PRIMER_LEFT_45_TM=62.573
PRIMER_RIGHT_45_TM=60.576
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=841
PRIMER_PAIR_46_PENALTY=3.148895
PRIMER_LEFT_46_PENALTY=2.572863
PRIMER_RIGHT_46_PENALTY=0.576032
PRIMER_LEFT_46_SEQUENCE=AGCCAAAATCTGACCGCTTG
PRIMER_RIGHT_46_SEQUENCE=TATCTCCAAAGCGGGAAACA
PRIMER_LEFT_46=558,20
PRIMER_RIGHT_46=1400,20
PRIMER_LEFT_46_TM=62.573
PRIMER_RIGHT_46_TM=60.576
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=843
PRIMER_PAIR_47_PENALTY=3.161420
PRIMER_LEFT_47_PENALTY=1.297092
PRIMER_RIGHT_47_PENALTY=1.864328
PRIMER_LEFT_47_SEQUENCE=CAATGCATCATCTAGGCAACA
PRIMER_RIGHT_47_SEQUENCE=ATATCTCCAAAGCGGGAAAC
PRIMER_LEFT_47=707,21
PRIMER_RIGHT_47=1401,20
PRIMER_LEFT_47_TM=59.703
PRIMER_RIGHT_47_TM=58.136
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=2.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=695
PRIMER_PAIR_48_PENALTY=3.209211
PRIMER_LEFT_48_PENALTY=0.353748
PRIMER_RIGHT_48_PENALTY=2.855463
PRIMER_LEFT_48_SEQUENCE=CCAATGCATCATCTAGGCAA
PRIMER_RIGHT_48_SEQUENCE=ACTAAATATCTCCAAAGCGGGA
PRIMER_LEFT_48=706,20
PRIMER_RIGHT_48=1406,22
PRIMER_LEFT_48_TM=59.646
PRIMER_RIGHT_48_TM=59.145
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=10.0000
PRIMER_RIGHT_48_END_STABILITY=11.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=701
PRIMER_PAIR_49_PENALTY=3.290554
PRIMER_LEFT_49_PENALTY=1.483814
PRIMER_RIGHT_49_PENALTY=1.806740
PRIMER_LEFT_49_SEQUENCE=CCAATGCATCATCTAGGCAAC
PRIMER_RIGHT_49_SEQUENCE=ATATCTCCAAAGCGGGAAACA
PRIMER_LEFT_49=706,21
PRIMER_RIGHT_49=1401,21
PRIMER_LEFT_49_TM=60.484
PRIMER_RIGHT_49_TM=60.807
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=696
=
SEQUENCE_ID=MAGIC.426_3_13977758_13978043_oy-0_wil-2_83275_29729_TRUE
SEQUENCE_TARGET=950,385
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_6466292_6466492_wu-0_bur-0_42043_24442_TRUE
SEQUENCE_TARGET=950,300
SEQUENCE_TEMPLATE=TGTGGAGTAGACGATATTGAGCAGATTACATCTGTTTCGTTTTCTCNNNNNNNNNNNNNNNNNNNNNNNTTNAATNTGTTTTGTTTCCTCCTTTTTATATTGNNNNNNNNNNNNNNNNNNNTATGAATTAAAAACTATATAATACAAAGCTCACATATATGCANGCAGACAAGTTTCTGGTTTAGATCTTGAGATATCATTCCAATATCAGTAAAGAAGATTTTGCGTATATTACCAGTAAAGAGTAAAGAGACTAATATCATCAGATCAACATTGACCTATCTATCTTTTTACTGTCACTATTAGTAACCGTTATTCTAAAGTACATTTGTATATTATATAGTNAATTATGTGACTTTGGTTGCAATCATTTCCAAAAATTAGGTAATTTACTGAAAGGAAAAGAAANAAACGTTTTCAGATATATTTTGATTTTTCTCTTAAAAAACAGTGAGAATTATGAATGNAGCGACACGACACATGTGANAAAAGCTCAATAATACATGAGAATTATAGAGCAACAAAACACAACACTGCTTTACAACTATCAAATACTAGTGTCGCCAAACCATCATAATAGANATACAACTCAACAAATGTCCTCTTGATTATAATCAGAATTATATATTATTATATGNTACAATACCTATCAAGTTTTGTTTTTGGTTGTAATCAAATTATTTCTAGGACGANATGATTGTCCCTACAAAGCAATTGGTAAGGATATGGTAAGAGGCACAATCTCTCTTTATACACAAAGGAAGGACTCATGGGATTGGATAAGCTCTTTCTGGGTCCCTAATTCCTCATTTCATTTTCAGAGAAAGAAGTTTCCAAAACCAACATTTAGTGATCTCTATATATTATATTCCTATTCCCCACTAAGTATCATCAAAGCTATATATAAACACCACTTTGAATCACCCAAGGCAATAAACCAAACCAAAACTTCCTAAGAAAAAAAAACAGAGAGATTTTTTTTNTCNTTCTAAAGCTTTTTNATAGAGTAAAGATGTTTTCAGAGCACTTACCTTCTTCTTCATCTCAAGTTTTCCAAGAACACTTCATNGACAGTTTCGTCTCCAGAAAATTGCTTCAGCAGATCCCATTTGCCCACAACGCTCAACAAGCTCATGTACCTGATAAGAACAACTTGAGCGGCAATGTTCTCATGCTGCTCTCTATCCTTTTGTGCGGTATNATNNGCTCCCTCGGGTTGCATTACATAATACGTTGTGCATTTATACGTTCTCGAAGTTTCATGATCTCTGATCCCATCTCTATCCCATCCACACCAAGANATTCATCAGTAAACAAAGGGATCAAGAAGAAAGCTCTCAAGATGTTACCAGTTGTGAATTACTCACCTGAAATTAACCTGCCAGGGGTCGGTGAAGAGTGTGTCATCTGCTTGTCTGATTTTGTTGCTGGAGAACAGCTACGTGTACTTCCCAAGTGCAACCATGGATTCCACCTTCGCTGCATCGACAAATGGCTTACGCAACATATGACTTGTCCGAAATGCAGACACTGTCTGGTTGATACATGCCAAAAAGTCTTAAGTNACTGCGATGCAGCTGATCAAGTGGCAGCGACAGCAACGGAGAGCATAGATATCAGAATTTCTCCTCTAGAACCTGAAGCAAGAGTAGCCACTTTTAGAGAAAGCAGCTAGAGTATGCCAACAAAACAATCATAATTTCAAANTTTGGCTCTCCAGCAAGATTATAGTTTCTTTATGTCACTAGCCATAGGATGTCAAAATCAAACANAGCAGAGNTTTTTTTTTGCCTTTTGTTTTTCTCAAAGAGTACAGAACATATACCTTAAGCTTTCTGTGGCATCATACGTTCATCAGAATTATCTNAATCTCTACTTCTTTGCAATACTCACAGCCAAGACTNACAATCGAAATTGTCATCTGCCAAGAAGCTTTTCAGAAATTTCCTCTGCATATTCAAATCACAATCACAGAGTAGAACTAGTAACTAAGGTCTGGAAAGAAATGGTTTCANTGAAGCTTTGGGCACCAAGACCAAGGTGCATGAAGAAGCAGTACTAAGACTACAGAATGTGATCTCAGTCTGACCTAATCGGTTACATATCTATTCACTCACTCACCAACTTGGTAGACTAACACCATCAATAGCCCCATTTGACCCACAGTGAGTTATTTGGTTAAGAATAAATAAGGGATTCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.373044
PRIMER_LEFT_0_PENALTY=0.137602
PRIMER_RIGHT_0_PENALTY=0.235442
PRIMER_LEFT_0_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_0_SEQUENCE=TGGATGGGATAGAGATGGGA
PRIMER_LEFT_0=760,20
PRIMER_RIGHT_0=1291,20
PRIMER_LEFT_0_TM=59.862
PRIMER_RIGHT_0_TM=60.235
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=532
PRIMER_PAIR_1_PENALTY=0.426034
PRIMER_LEFT_1_PENALTY=0.137602
PRIMER_RIGHT_1_PENALTY=0.288431
PRIMER_LEFT_1_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_1_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_1=760,20
PRIMER_RIGHT_1=1405,20
PRIMER_LEFT_1_TM=59.862
PRIMER_RIGHT_1_TM=59.712
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=646
PRIMER_PAIR_2_PENALTY=0.519816
PRIMER_LEFT_2_PENALTY=0.284374
PRIMER_RIGHT_2_PENALTY=0.235442
PRIMER_LEFT_2_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_2_SEQUENCE=TGGATGGGATAGAGATGGGA
PRIMER_LEFT_2=554,20
PRIMER_RIGHT_2=1291,20
PRIMER_LEFT_2_TM=59.716
PRIMER_RIGHT_2_TM=60.235
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=738
PRIMER_PAIR_3_PENALTY=0.572805
PRIMER_LEFT_3_PENALTY=0.284374
PRIMER_RIGHT_3_PENALTY=0.288431
PRIMER_LEFT_3_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_3_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_3=554,20
PRIMER_RIGHT_3=1405,20
PRIMER_LEFT_3_TM=59.716
PRIMER_RIGHT_3_TM=59.712
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=852
PRIMER_PAIR_4_PENALTY=0.710343
PRIMER_LEFT_4_PENALTY=0.137602
PRIMER_RIGHT_4_PENALTY=0.572741
PRIMER_LEFT_4_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_4_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_4=760,20
PRIMER_RIGHT_4=1404,20
PRIMER_LEFT_4_TM=59.862
PRIMER_RIGHT_4_TM=60.573
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=645
PRIMER_PAIR_5_PENALTY=0.757235
PRIMER_LEFT_5_PENALTY=0.521793
PRIMER_RIGHT_5_PENALTY=0.235442
PRIMER_LEFT_5_SEQUENCE=TCTGGGTCCCTAATTCCTCA
PRIMER_RIGHT_5_SEQUENCE=TGGATGGGATAGAGATGGGA
PRIMER_LEFT_5=789,20
PRIMER_RIGHT_5=1291,20
PRIMER_LEFT_5_TM=59.478
PRIMER_RIGHT_5_TM=60.235
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=503
PRIMER_PAIR_6_PENALTY=0.783604
PRIMER_LEFT_6_PENALTY=0.495173
PRIMER_RIGHT_6_PENALTY=0.288431
PRIMER_LEFT_6_SEQUENCE=ACTTTGAATCACCCAAGGCA
PRIMER_RIGHT_6_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_6=912,20
PRIMER_RIGHT_6=1405,20
PRIMER_LEFT_6_TM=60.495
PRIMER_RIGHT_6_TM=59.712
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=494
PRIMER_PAIR_7_PENALTY=0.810224
PRIMER_LEFT_7_PENALTY=0.521793
PRIMER_RIGHT_7_PENALTY=0.288431
PRIMER_LEFT_7_SEQUENCE=TCTGGGTCCCTAATTCCTCA
PRIMER_RIGHT_7_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_7=789,20
PRIMER_RIGHT_7=1405,20
PRIMER_LEFT_7_TM=59.478
PRIMER_RIGHT_7_TM=59.712
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=617
PRIMER_PAIR_8_PENALTY=0.857115
PRIMER_LEFT_8_PENALTY=0.284374
PRIMER_RIGHT_8_PENALTY=0.572741
PRIMER_LEFT_8_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_8_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_8=554,20
PRIMER_RIGHT_8=1404,20
PRIMER_LEFT_8_TM=59.716
PRIMER_RIGHT_8_TM=60.573
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=60.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=9.1000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=851
PRIMER_PAIR_9_PENALTY=0.970647
PRIMER_LEFT_9_PENALTY=0.137602
PRIMER_RIGHT_9_PENALTY=0.833045
PRIMER_LEFT_9_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_9_SEQUENCE=GTGGATGGGATAGAGATGGG
PRIMER_LEFT_9=760,20
PRIMER_RIGHT_9=1292,20
PRIMER_LEFT_9_TM=59.862
PRIMER_RIGHT_9_TM=59.167
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=533
PRIMER_PAIR_10_PENALTY=0.973021
PRIMER_LEFT_10_PENALTY=0.684590
PRIMER_RIGHT_10_PENALTY=0.288431
PRIMER_LEFT_10_SEQUENCE=CCAAGGCAATAAACCAAACC
PRIMER_RIGHT_10_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_10=924,20
PRIMER_RIGHT_10=1405,20
PRIMER_LEFT_10_TM=59.315
PRIMER_RIGHT_10_TM=59.712
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=482
PRIMER_PAIR_11_PENALTY=0.973021
PRIMER_LEFT_11_PENALTY=0.684590
PRIMER_RIGHT_11_PENALTY=0.288431
PRIMER_LEFT_11_SEQUENCE=CCCAAGGCAATAAACCAAAC
PRIMER_RIGHT_11_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_11=923,20
PRIMER_RIGHT_11=1405,20
PRIMER_LEFT_11_TM=59.315
PRIMER_RIGHT_11_TM=59.712
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=483
PRIMER_PAIR_12_PENALTY=0.973021
PRIMER_LEFT_12_PENALTY=0.684590
PRIMER_RIGHT_12_PENALTY=0.288431
PRIMER_LEFT_12_SEQUENCE=ACCCAAGGCAATAAACCAAA
PRIMER_RIGHT_12_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_12=922,20
PRIMER_RIGHT_12=1405,20
PRIMER_LEFT_12_TM=59.315
PRIMER_RIGHT_12_TM=59.712
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.8000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=484
PRIMER_PAIR_13_PENALTY=1.010745
PRIMER_LEFT_13_PENALTY=0.722314
PRIMER_RIGHT_13_PENALTY=0.288431
PRIMER_LEFT_13_SEQUENCE=CACCCAAGGCAATAAACCAA
PRIMER_RIGHT_13_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_13=921,20
PRIMER_RIGHT_13=1405,20
PRIMER_LEFT_13_TM=60.722
PRIMER_RIGHT_13_TM=59.712
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=485
PRIMER_PAIR_14_PENALTY=1.067913
PRIMER_LEFT_14_PENALTY=0.495173
PRIMER_RIGHT_14_PENALTY=0.572741
PRIMER_LEFT_14_SEQUENCE=ACTTTGAATCACCCAAGGCA
PRIMER_RIGHT_14_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_14=912,20
PRIMER_RIGHT_14=1404,20
PRIMER_LEFT_14_TM=60.495
PRIMER_RIGHT_14_TM=60.573
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=60.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=493
PRIMER_PAIR_15_PENALTY=1.087539
PRIMER_LEFT_15_PENALTY=0.137602
PRIMER_RIGHT_15_PENALTY=0.949937
PRIMER_LEFT_15_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_15_SEQUENCE=GCTGTTCTCCAGCAACAAAA
PRIMER_LEFT_15=760,20
PRIMER_RIGHT_15=1438,20
PRIMER_LEFT_15_TM=59.862
PRIMER_RIGHT_15_TM=59.050
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=679
PRIMER_PAIR_16_PENALTY=1.094534
PRIMER_LEFT_16_PENALTY=0.521793
PRIMER_RIGHT_16_PENALTY=0.572741
PRIMER_LEFT_16_SEQUENCE=TCTGGGTCCCTAATTCCTCA
PRIMER_RIGHT_16_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_16=789,20
PRIMER_RIGHT_16=1404,20
PRIMER_LEFT_16_TM=59.478
PRIMER_RIGHT_16_TM=60.573
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=60.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=616
PRIMER_PAIR_17_PENALTY=1.117419
PRIMER_LEFT_17_PENALTY=0.284374
PRIMER_RIGHT_17_PENALTY=0.833045
PRIMER_LEFT_17_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_17_SEQUENCE=GTGGATGGGATAGAGATGGG
PRIMER_LEFT_17=554,20
PRIMER_RIGHT_17=1292,20
PRIMER_LEFT_17_TM=59.716
PRIMER_RIGHT_17_TM=59.167
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=739
PRIMER_PAIR_18_PENALTY=1.157753
PRIMER_LEFT_18_PENALTY=0.137602
PRIMER_RIGHT_18_PENALTY=1.020151
PRIMER_LEFT_18_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_18_SEQUENCE=TTGGCATGTATCAACCAGACA
PRIMER_LEFT_18=760,20
PRIMER_RIGHT_18=1551,21
PRIMER_LEFT_18_TM=59.862
PRIMER_RIGHT_18_TM=59.980
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=792
PRIMER_PAIR_19_PENALTY=1.162772
PRIMER_LEFT_19_PENALTY=0.137602
PRIMER_RIGHT_19_PENALTY=1.025170
PRIMER_LEFT_19_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_19_SEQUENCE=ATGACACACTCTTCACCGACC
PRIMER_LEFT_19=760,20
PRIMER_RIGHT_19=1406,21
PRIMER_LEFT_19_TM=59.862
PRIMER_RIGHT_19_TM=60.025
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=647
PRIMER_PAIR_20_PENALTY=1.182389
PRIMER_LEFT_20_PENALTY=0.137602
PRIMER_RIGHT_20_PENALTY=1.044787
PRIMER_LEFT_20_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_20_SEQUENCE=AGCTGTTCTCCAGCAACAAAA
PRIMER_LEFT_20=760,20
PRIMER_RIGHT_20=1439,21
PRIMER_LEFT_20_TM=59.862
PRIMER_RIGHT_20_TM=60.045
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=680
PRIMER_PAIR_21_PENALTY=1.234311
PRIMER_LEFT_21_PENALTY=0.284374
PRIMER_RIGHT_21_PENALTY=0.949937
PRIMER_LEFT_21_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_21_SEQUENCE=GCTGTTCTCCAGCAACAAAA
PRIMER_LEFT_21=554,20
PRIMER_RIGHT_21=1438,20
PRIMER_LEFT_21_TM=59.716
PRIMER_RIGHT_21_TM=59.050
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=885
PRIMER_PAIR_22_PENALTY=1.257330
PRIMER_LEFT_22_PENALTY=0.684590
PRIMER_RIGHT_22_PENALTY=0.572741
PRIMER_LEFT_22_SEQUENCE=CCAAGGCAATAAACCAAACC
PRIMER_RIGHT_22_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_22=924,20
PRIMER_RIGHT_22=1404,20
PRIMER_LEFT_22_TM=59.315
PRIMER_RIGHT_22_TM=60.573
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=60.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=9.1000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=481
PRIMER_PAIR_23_PENALTY=1.257330
PRIMER_LEFT_23_PENALTY=0.684590
PRIMER_RIGHT_23_PENALTY=0.572741
PRIMER_LEFT_23_SEQUENCE=CCCAAGGCAATAAACCAAAC
PRIMER_RIGHT_23_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_23=923,20
PRIMER_RIGHT_23=1404,20
PRIMER_LEFT_23_TM=59.315
PRIMER_RIGHT_23_TM=60.573
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=60.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=9.1000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=482
PRIMER_PAIR_24_PENALTY=1.257330
PRIMER_LEFT_24_PENALTY=0.684590
PRIMER_RIGHT_24_PENALTY=0.572741
PRIMER_LEFT_24_SEQUENCE=ACCCAAGGCAATAAACCAAA
PRIMER_RIGHT_24_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_24=922,20
PRIMER_RIGHT_24=1404,20
PRIMER_LEFT_24_TM=59.315
PRIMER_RIGHT_24_TM=60.573
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=60.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=9.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=483
PRIMER_PAIR_25_PENALTY=1.259611
PRIMER_LEFT_25_PENALTY=0.137602
PRIMER_RIGHT_25_PENALTY=1.122009
PRIMER_LEFT_25_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_25_SEQUENCE=TGGTGTGGATGGGATAGAGA
PRIMER_LEFT_25=760,20
PRIMER_RIGHT_25=1296,20
PRIMER_LEFT_25_TM=59.862
PRIMER_RIGHT_25_TM=58.878
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=537
PRIMER_PAIR_26_PENALTY=1.289232
PRIMER_LEFT_26_PENALTY=1.000801
PRIMER_RIGHT_26_PENALTY=0.288431
PRIMER_LEFT_26_SEQUENCE=CTTTGAATCACCCAAGGCAA
PRIMER_RIGHT_26_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_26=913,20
PRIMER_RIGHT_26=1405,20
PRIMER_LEFT_26_TM=61.001
PRIMER_RIGHT_26_TM=59.712
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.0000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=493
PRIMER_PAIR_27_PENALTY=1.294979
PRIMER_LEFT_27_PENALTY=1.059537
PRIMER_RIGHT_27_PENALTY=0.235442
PRIMER_LEFT_27_SEQUENCE=AGGAAGGACTCATGGGATTG
PRIMER_RIGHT_27_SEQUENCE=TGGATGGGATAGAGATGGGA
PRIMER_LEFT_27=758,20
PRIMER_RIGHT_27=1291,20
PRIMER_LEFT_27_TM=58.940
PRIMER_RIGHT_27_TM=60.235
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=534
PRIMER_PAIR_28_PENALTY=1.295055
PRIMER_LEFT_28_PENALTY=0.722314
PRIMER_RIGHT_28_PENALTY=0.572741
PRIMER_LEFT_28_SEQUENCE=CACCCAAGGCAATAAACCAA
PRIMER_RIGHT_28_SEQUENCE=GACACACTCTTCACCGACCC
PRIMER_LEFT_28=921,20
PRIMER_RIGHT_28=1404,20
PRIMER_LEFT_28_TM=60.722
PRIMER_RIGHT_28_TM=60.573
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=60.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=9.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=484
PRIMER_PAIR_29_PENALTY=1.304525
PRIMER_LEFT_29_PENALTY=0.284374
PRIMER_RIGHT_29_PENALTY=1.020151
PRIMER_LEFT_29_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_29_SEQUENCE=TTGGCATGTATCAACCAGACA
PRIMER_LEFT_29=554,20
PRIMER_RIGHT_29=1551,21
PRIMER_LEFT_29_TM=59.716
PRIMER_RIGHT_29_TM=59.980
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=998
PRIMER_PAIR_30_PENALTY=1.309544
PRIMER_LEFT_30_PENALTY=0.284374
PRIMER_RIGHT_30_PENALTY=1.025170
PRIMER_LEFT_30_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_30_SEQUENCE=ATGACACACTCTTCACCGACC
PRIMER_LEFT_30=554,20
PRIMER_RIGHT_30=1406,21
PRIMER_LEFT_30_TM=59.716
PRIMER_RIGHT_30_TM=60.025
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=9.6000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=853
PRIMER_PAIR_31_PENALTY=1.323121
PRIMER_LEFT_31_PENALTY=1.034689
PRIMER_RIGHT_31_PENALTY=0.288431
PRIMER_LEFT_31_SEQUENCE=TGAATCACCCAAGGCAATAA
PRIMER_RIGHT_31_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_31=916,20
PRIMER_RIGHT_31=1405,20
PRIMER_LEFT_31_TM=58.965
PRIMER_RIGHT_31_TM=59.712
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.2000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=490
PRIMER_PAIR_32_PENALTY=1.329161
PRIMER_LEFT_32_PENALTY=0.284374
PRIMER_RIGHT_32_PENALTY=1.044787
PRIMER_LEFT_32_SEQUENCE=CTAGTGTCGCCAAACCATCA
PRIMER_RIGHT_32_SEQUENCE=AGCTGTTCTCCAGCAACAAAA
PRIMER_LEFT_32=554,20
PRIMER_RIGHT_32=1439,21
PRIMER_LEFT_32_TM=59.716
PRIMER_RIGHT_32_TM=60.045
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=886
PRIMER_PAIR_33_PENALTY=1.347968
PRIMER_LEFT_33_PENALTY=1.059537
PRIMER_RIGHT_33_PENALTY=0.288431
PRIMER_LEFT_33_SEQUENCE=AGGAAGGACTCATGGGATTG
PRIMER_RIGHT_33_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_33=758,20
PRIMER_RIGHT_33=1405,20
PRIMER_LEFT_33_TM=58.940
PRIMER_RIGHT_33_TM=59.712
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=648
PRIMER_PAIR_34_PENALTY=1.354838
PRIMER_LEFT_34_PENALTY=0.521793
PRIMER_RIGHT_34_PENALTY=0.833045
PRIMER_LEFT_34_SEQUENCE=TCTGGGTCCCTAATTCCTCA
PRIMER_RIGHT_34_SEQUENCE=GTGGATGGGATAGAGATGGG
PRIMER_LEFT_34=789,20
PRIMER_RIGHT_34=1292,20
PRIMER_LEFT_34_TM=59.478
PRIMER_RIGHT_34_TM=59.167
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=504
PRIMER_PAIR_35_PENALTY=1.356580
PRIMER_LEFT_35_PENALTY=1.068149
PRIMER_RIGHT_35_PENALTY=0.288431
PRIMER_LEFT_35_SEQUENCE=AATCACCCAAGGCAATAAACC
PRIMER_RIGHT_35_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_35=918,21
PRIMER_RIGHT_35=1405,20
PRIMER_LEFT_35_TM=60.068
PRIMER_RIGHT_35_TM=59.712
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=9.6000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=488
PRIMER_PAIR_36_PENALTY=1.390638
PRIMER_LEFT_36_PENALTY=1.102207
PRIMER_RIGHT_36_PENALTY=0.288431
PRIMER_LEFT_36_SEQUENCE=ACCCAAGGCAATAAACCAAAC
PRIMER_RIGHT_36_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_36=922,21
PRIMER_RIGHT_36=1405,20
PRIMER_LEFT_36_TM=60.102
PRIMER_RIGHT_36_TM=59.712
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=9.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=484
PRIMER_PAIR_37_PENALTY=1.445109
PRIMER_LEFT_37_PENALTY=0.495173
PRIMER_RIGHT_37_PENALTY=0.949937
PRIMER_LEFT_37_SEQUENCE=ACTTTGAATCACCCAAGGCA
PRIMER_RIGHT_37_SEQUENCE=GCTGTTCTCCAGCAACAAAA
PRIMER_LEFT_37=912,20
PRIMER_RIGHT_37=1438,20
PRIMER_LEFT_37_TM=60.495
PRIMER_RIGHT_37_TM=59.050
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=527
PRIMER_PAIR_38_PENALTY=1.461380
PRIMER_LEFT_38_PENALTY=1.172949
PRIMER_RIGHT_38_PENALTY=0.288431
PRIMER_LEFT_38_SEQUENCE=ACACCACTTTGAATCACCCA
PRIMER_RIGHT_38_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_38=907,20
PRIMER_RIGHT_38=1405,20
PRIMER_LEFT_38_TM=58.827
PRIMER_RIGHT_38_TM=59.712
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=499
PRIMER_PAIR_39_PENALTY=1.461826
PRIMER_LEFT_39_PENALTY=0.137602
PRIMER_RIGHT_39_PENALTY=1.324224
PRIMER_LEFT_39_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_39_SEQUENCE=TTGGTGTGGATGGGATAGAGA
PRIMER_LEFT_39=760,20
PRIMER_RIGHT_39=1297,21
PRIMER_LEFT_39_TM=59.862
PRIMER_RIGHT_39_TM=60.324
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=538
PRIMER_PAIR_40_PENALTY=1.461826
PRIMER_LEFT_40_PENALTY=0.137602
PRIMER_RIGHT_40_PENALTY=1.324224
PRIMER_LEFT_40_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_40_SEQUENCE=TCTTGGTGTGGATGGGATAGA
PRIMER_LEFT_40=760,20
PRIMER_RIGHT_40=1299,21
PRIMER_LEFT_40_TM=59.862
PRIMER_RIGHT_40_TM=60.324
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=5.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=540
PRIMER_PAIR_41_PENALTY=1.471730
PRIMER_LEFT_41_PENALTY=0.521793
PRIMER_RIGHT_41_PENALTY=0.949937
PRIMER_LEFT_41_SEQUENCE=TCTGGGTCCCTAATTCCTCA
PRIMER_RIGHT_41_SEQUENCE=GCTGTTCTCCAGCAACAAAA
PRIMER_LEFT_41=789,20
PRIMER_RIGHT_41=1438,20
PRIMER_LEFT_41_TM=59.478
PRIMER_RIGHT_41_TM=59.050
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=650
PRIMER_PAIR_42_PENALTY=1.512970
PRIMER_LEFT_42_PENALTY=0.137602
PRIMER_RIGHT_42_PENALTY=1.375368
PRIMER_LEFT_42_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_42_SEQUENCE=CTATGCTCTCCGTTGCTGTC
PRIMER_LEFT_42=760,20
PRIMER_RIGHT_42=1612,20
PRIMER_LEFT_42_TM=59.862
PRIMER_RIGHT_42_TM=58.625
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=853
PRIMER_PAIR_43_PENALTY=1.512970
PRIMER_LEFT_43_PENALTY=0.137602
PRIMER_RIGHT_43_PENALTY=1.375368
PRIMER_LEFT_43_SEQUENCE=GAAGGACTCATGGGATTGGA
PRIMER_RIGHT_43_SEQUENCE=TCTATGCTCTCCGTTGCTGT
PRIMER_LEFT_43=760,20
PRIMER_RIGHT_43=1613,20
PRIMER_LEFT_43_TM=59.862
PRIMER_RIGHT_43_TM=58.625
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=854
PRIMER_PAIR_44_PENALTY=1.515323
PRIMER_LEFT_44_PENALTY=0.495173
PRIMER_RIGHT_44_PENALTY=1.020151
PRIMER_LEFT_44_SEQUENCE=ACTTTGAATCACCCAAGGCA
PRIMER_RIGHT_44_SEQUENCE=TTGGCATGTATCAACCAGACA
PRIMER_LEFT_44=912,20
PRIMER_RIGHT_44=1551,21
PRIMER_LEFT_44_TM=60.495
PRIMER_RIGHT_44_TM=59.980
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.7000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=640
PRIMER_PAIR_45_PENALTY=1.520342
PRIMER_LEFT_45_PENALTY=0.495173
PRIMER_RIGHT_45_PENALTY=1.025170
PRIMER_LEFT_45_SEQUENCE=ACTTTGAATCACCCAAGGCA
PRIMER_RIGHT_45_SEQUENCE=ATGACACACTCTTCACCGACC
PRIMER_LEFT_45=912,20
PRIMER_RIGHT_45=1406,21
PRIMER_LEFT_45_TM=60.495
PRIMER_RIGHT_45_TM=60.025
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.7000
PRIMER_RIGHT_45_END_STABILITY=9.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=495
PRIMER_PAIR_46_PENALTY=1.523780
PRIMER_LEFT_46_PENALTY=1.235348
PRIMER_RIGHT_46_PENALTY=0.288431
PRIMER_LEFT_46_SEQUENCE=ATCACCCAAGGCAATAAACC
PRIMER_RIGHT_46_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_46=919,20
PRIMER_RIGHT_46=1405,20
PRIMER_LEFT_46_TM=58.765
PRIMER_RIGHT_46_TM=59.712
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=9.6000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=487
PRIMER_PAIR_47_PENALTY=1.539959
PRIMER_LEFT_47_PENALTY=0.495173
PRIMER_RIGHT_47_PENALTY=1.044787
PRIMER_LEFT_47_SEQUENCE=ACTTTGAATCACCCAAGGCA
PRIMER_RIGHT_47_SEQUENCE=AGCTGTTCTCCAGCAACAAAA
PRIMER_LEFT_47=912,20
PRIMER_RIGHT_47=1439,21
PRIMER_LEFT_47_TM=60.495
PRIMER_RIGHT_47_TM=60.045
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=7.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=528
PRIMER_PAIR_48_PENALTY=1.541944
PRIMER_LEFT_48_PENALTY=0.521793
PRIMER_RIGHT_48_PENALTY=1.020151
PRIMER_LEFT_48_SEQUENCE=TCTGGGTCCCTAATTCCTCA
PRIMER_RIGHT_48_SEQUENCE=TTGGCATGTATCAACCAGACA
PRIMER_LEFT_48=789,20
PRIMER_RIGHT_48=1551,21
PRIMER_LEFT_48_TM=59.478
PRIMER_RIGHT_48_TM=59.980
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=763
PRIMER_PAIR_49_PENALTY=1.542183
PRIMER_LEFT_49_PENALTY=1.253752
PRIMER_RIGHT_49_PENALTY=0.288431
PRIMER_LEFT_49_SEQUENCE=TCACCCAAGGCAATAAACCA
PRIMER_RIGHT_49_SEQUENCE=TGACACACTCTTCACCGACC
PRIMER_LEFT_49=920,20
PRIMER_RIGHT_49=1405,20
PRIMER_LEFT_49_TM=61.254
PRIMER_RIGHT_49_TM=59.712
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=486
=
SEQUENCE_ID=MAGIC.105_4_10734669_10735095_po-0_bur-0_34686_14112_TRUE
SEQUENCE_TARGET=950,526
SEQUENCE_TEMPLATE=ATATGAGAGACTCGGTAGTGTTGAATTAATCCCATATATAGGATTTATAATTTTTGCTGCAATTCANAAACGAAACNNANNANNNNNTATTTATGNANAAAAAGAAAGAGGCNAACCTGCATTAGANAGNANAGTGTTATCGTCAGCTCCAACGTTCCATGCAAAGTAACCAAGCAGACCGTTCTGCTTANCGTATCNCACTTTGGCCTCGACGCTCTGAGTATCTTCGTACCCAATCCAAATCTTCTTGGCGAAACAATAATGTCCGACAACCTTGGGGTCATANACCTTNGCTGCTTCCTNATCCCNTATGAACTTGTTTATCTNAGCATAGTTTATAGAACCGTCCTCAGATACGTCATCCTCAGATTTGGCTACGCCGNCAGCGGCCGCATTATAACCGGTATNTTTATCGTTNTGGAGAGTCCAGGCCCANCCAACGTAGGAGAATCNCAAGACTGCTTTCTTCTCGGGAAGTCCGTCCTTAATCCACTGTNTCAAACCAGAGNNGCCGCTTGGACCTATCAACCAAAGTACCAATATATAGTTCCAAGTTAAANAAAANAAAAAAAANTTGATCACCNAANTATCCCTTCTTNANTNATATACTACAACTNTATATGCTTAATTATACCTTNGTTGTTAGAAGAAACGTGCAGACCGGCGGTTGGAACGGTAAATTTGGAGGACGACACCGGTCCGTAAAAATCATAGGCTATAATATTAACCCAATCCAAGCTTTCTCTCATCACTTGAACCGGGTAGGTAAANGTCTTGTAAACCGAGGAGTAGTAAACCGCAGCGGTCAACAGCAAAGTCGGTTTGCTGCTGCGTCTGCTCTCGGCCTCAACCGCTGCTCGCAATTCTCCAACTAGATTCCCAAAGTNGGTCATCTCATGATCACTGTNCGGGTACTCCNAGGCTAAATCGAGACCATGGAATCCNTTGGATCTAGCGATAAAAATCCAAGAATCAATGAACGTTTNCCGAGACTGGTGGTNACTTGCCATGGACGCAAACGCAGAATTGTTTGCATTTTTNCCGCCGATNGACAAGAGGGTCTTCACCTGAGGGTTTCGAATCTTGACGGTTTCCGTGAAGGTGGAAAANATGAATTCGTGCGCTTGGGAGACAAAAACTTTGTGGGAGTTAGCGTCGAGATCGGCGAAGGCGCAGAAAAGGTGAGTGAAGAGAGCTGAGGGAATGGTTTCGGCCGAGGTTATGGGGTCCTGGGTCTCTCCATCTGGGAACCAGTACGAAGCTTTTACCTCGGCGGAGGGGGATCCCAGAATCACAGTAGAAGTCATTTTGGTCTCAAAGTGTTTCTTTGCTTTCTCTTTGTGTTTCTTTGCTTGTTTTGCTTTGTAGTAGTACCTAAGAGGGTGCTTCCCTTCTCTATTTATAGGGATTTTGGAAATATANNTTTNNNAAAANAATTAATCCAGACTAATTCAATTNTTGAAAACCAAAGGTCTTTGCTAAATTANTGCTGTCACTGTGACACCATTCATTAAGAAGCTCATTCNTATTATACTTNATGTATTCTTATTTAACACNTTTTGTGAAGTTTAAACCCNAATTTATTGTTGAGANGTCGTTGAAGAGNNNTTTATCTTGCTAGTTTTTAAAACTAGGGNCCGTCTGACNTTTTCCACGGTGATTTTNATTANGTNTTCATGCTATCAATGTCATGNATACACACCCTTTTACACACATACNNGATCATANTTANTCTGCTGNTATTAGGCCTTCAGCTTANGTATGATTCATCTANTTTGTCTAACAATCTANTGGTTCACAACTTCACANNNATTAAAAATTATATAGTAATNTGNAAAAAANCGTATTCACTNAAAATTGNACTTCATACCTGGTGTCTAATTATTAATCAAACCATTGGTCAAACACTTTACTAAAGAAACTTCCACNTTTAGNNATANTTANTCTCATTCTATAAAATTNTGNNAAAATANATTTGTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNTGACTGTANAAATATTTATNGNTGTTTAAATGTGTCAAATTTGNAAACNCATATNTGGCNACNTCNATATTTCCGANTACATATACNTTNGTGTGCGTGNATGTTTGAGNATAAAGNAAACTACACAGANAGTTTGTTTNANGNTTCGNTTTTCCTACATCCCTTGGANCTATNTCATAACAAANCNACTANNNNNNNNNNNNAATTACAATTNCACATGATNNNGAATACTANTATGNCTATCATTNNTTNTANATGAATAAATCACNAATATATTTTACATCATATNTGGTGGGNTGNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATANCTTGAAGCNTGCANATCTTCATNAAACGACATCTTGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=6.527219
PRIMER_LEFT_0_PENALTY=0.156287
PRIMER_RIGHT_0_PENALTY=6.370931
PRIMER_LEFT_0_SEQUENCE=GGTCAACAGCAAAGTCGGTT
PRIMER_RIGHT_0_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_0=803,20
PRIMER_RIGHT_0=1520,22
PRIMER_LEFT_0_TM=60.156
PRIMER_RIGHT_0_TM=55.629
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=718
PRIMER_PAIR_1_PENALTY=6.576245
PRIMER_LEFT_1_PENALTY=0.205314
PRIMER_RIGHT_1_PENALTY=6.370931
PRIMER_LEFT_1_SEQUENCE=TGGAACGGTAAATTTGGAGG
PRIMER_RIGHT_1_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_1=668,20
PRIMER_RIGHT_1=1520,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=55.629
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=853
PRIMER_PAIR_2_PENALTY=6.633338
PRIMER_LEFT_2_PENALTY=0.262407
PRIMER_RIGHT_2_PENALTY=6.370931
PRIMER_LEFT_2_SEQUENCE=CCGAGGAGTAGTAAACCGCA
PRIMER_RIGHT_2_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_2=781,20
PRIMER_RIGHT_2=1520,22
PRIMER_LEFT_2_TM=60.262
PRIMER_RIGHT_2_TM=55.629
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=740
PRIMER_PAIR_3_PENALTY=6.909015
PRIMER_LEFT_3_PENALTY=0.538083
PRIMER_RIGHT_3_PENALTY=6.370931
PRIMER_LEFT_3_SEQUENCE=CTGCTCGCAATTCTCCAACT
PRIMER_RIGHT_3_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_3=853,20
PRIMER_RIGHT_3=1520,22
PRIMER_LEFT_3_TM=60.538
PRIMER_RIGHT_3_TM=55.629
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=668
PRIMER_PAIR_4_PENALTY=6.941752
PRIMER_LEFT_4_PENALTY=0.570821
PRIMER_RIGHT_4_PENALTY=6.370931
PRIMER_LEFT_4_SEQUENCE=ACGGTAAATTTGGAGGACGA
PRIMER_RIGHT_4_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_4=672,20
PRIMER_RIGHT_4=1520,22
PRIMER_LEFT_4_TM=59.429
PRIMER_RIGHT_4_TM=55.629
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=849
PRIMER_PAIR_5_PENALTY=7.491868
PRIMER_LEFT_5_PENALTY=1.120937
PRIMER_RIGHT_5_PENALTY=6.370931
PRIMER_LEFT_5_SEQUENCE=GGAACGGTAAATTTGGAGGA
PRIMER_RIGHT_5_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_5=669,20
PRIMER_RIGHT_5=1520,22
PRIMER_LEFT_5_TM=58.879
PRIMER_RIGHT_5_TM=55.629
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.1000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=852
PRIMER_PAIR_6_PENALTY=7.509705
PRIMER_LEFT_6_PENALTY=1.138774
PRIMER_RIGHT_6_PENALTY=6.370931
PRIMER_LEFT_6_SEQUENCE=CCCAATCCAAGCTTTCTCTC
PRIMER_RIGHT_6_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_6=727,20
PRIMER_RIGHT_6=1520,22
PRIMER_LEFT_6_TM=58.861
PRIMER_RIGHT_6_TM=55.629
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=794
PRIMER_PAIR_7_PENALTY=7.666393
PRIMER_LEFT_7_PENALTY=1.295461
PRIMER_RIGHT_7_PENALTY=6.370931
PRIMER_LEFT_7_SEQUENCE=ACCCAATCCAAGCTTTCTCTC
PRIMER_RIGHT_7_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_7=726,21
PRIMER_RIGHT_7=1520,22
PRIMER_LEFT_7_TM=59.705
PRIMER_RIGHT_7_TM=55.629
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.1000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=795
PRIMER_PAIR_8_PENALTY=7.675160
PRIMER_LEFT_8_PENALTY=1.304229
PRIMER_RIGHT_8_PENALTY=6.370931
PRIMER_LEFT_8_SEQUENCE=GGAACGGTAAATTTGGAGGAC
PRIMER_RIGHT_8_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_8=669,21
PRIMER_RIGHT_8=1520,22
PRIMER_LEFT_8_TM=59.696
PRIMER_RIGHT_8_TM=55.629
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=6.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=852
PRIMER_PAIR_9_PENALTY=7.769887
PRIMER_LEFT_9_PENALTY=1.398956
PRIMER_RIGHT_9_PENALTY=6.370931
PRIMER_LEFT_9_SEQUENCE=CTCATCACTTGAACCGGGTAG
PRIMER_RIGHT_9_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_9=744,21
PRIMER_RIGHT_9=1520,22
PRIMER_LEFT_9_TM=59.601
PRIMER_RIGHT_9_TM=55.629
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=777
PRIMER_PAIR_10_PENALTY=7.802335
PRIMER_LEFT_10_PENALTY=1.431404
PRIMER_RIGHT_10_PENALTY=6.370931
PRIMER_LEFT_10_SEQUENCE=TCATCACTTGAACCGGGTAG
PRIMER_RIGHT_10_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_10=745,20
PRIMER_RIGHT_10=1520,22
PRIMER_LEFT_10_TM=58.569
PRIMER_RIGHT_10_TM=55.629
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=776
PRIMER_PAIR_11_PENALTY=8.078231
PRIMER_LEFT_11_PENALTY=1.707300
PRIMER_RIGHT_11_PENALTY=6.370931
PRIMER_LEFT_11_SEQUENCE=AACGGTAAATTTGGAGGACGA
PRIMER_RIGHT_11_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_11=671,21
PRIMER_RIGHT_11=1520,22
PRIMER_LEFT_11_TM=60.707
PRIMER_RIGHT_11_TM=55.629
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.1000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=850
PRIMER_PAIR_12_PENALTY=8.130553
PRIMER_LEFT_12_PENALTY=1.759622
PRIMER_RIGHT_12_PENALTY=6.370931
PRIMER_LEFT_12_SEQUENCE=AACCCAATCCAAGCTTTCTC
PRIMER_RIGHT_12_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_12=725,20
PRIMER_RIGHT_12=1520,22
PRIMER_LEFT_12_TM=58.240
PRIMER_RIGHT_12_TM=55.629
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=796
PRIMER_PAIR_13_PENALTY=8.144122
PRIMER_LEFT_13_PENALTY=1.773191
PRIMER_RIGHT_13_PENALTY=6.370931
PRIMER_LEFT_13_SEQUENCE=AACCCAATCCAAGCTTTCTCT
PRIMER_RIGHT_13_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_13=725,21
PRIMER_RIGHT_13=1520,22
PRIMER_LEFT_13_TM=59.227
PRIMER_RIGHT_13_TM=55.629
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.1000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=796
PRIMER_PAIR_14_PENALTY=8.295722
PRIMER_LEFT_14_PENALTY=1.924791
PRIMER_RIGHT_14_PENALTY=6.370931
PRIMER_LEFT_14_SEQUENCE=GGTTGGAACGGTAAATTTGGA
PRIMER_RIGHT_14_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_14=665,21
PRIMER_RIGHT_14=1520,22
PRIMER_LEFT_14_TM=60.925
PRIMER_RIGHT_14_TM=55.629
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.1000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=856
PRIMER_PAIR_15_PENALTY=8.342445
PRIMER_LEFT_15_PENALTY=1.971514
PRIMER_RIGHT_15_PENALTY=6.370931
PRIMER_LEFT_15_SEQUENCE=CAGCGGTCAACAGCAAAGTC
PRIMER_RIGHT_15_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_15=799,20
PRIMER_RIGHT_15=1520,22
PRIMER_LEFT_15_TM=61.972
PRIMER_RIGHT_15_TM=55.629
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=722
PRIMER_PAIR_16_PENALTY=8.350966
PRIMER_LEFT_16_PENALTY=1.980035
PRIMER_RIGHT_16_PENALTY=6.370931
PRIMER_LEFT_16_SEQUENCE=CCAATCCAAGCTTTCTCTCA
PRIMER_RIGHT_16_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_16=728,20
PRIMER_RIGHT_16=1520,22
PRIMER_LEFT_16_TM=58.020
PRIMER_RIGHT_16_TM=55.629
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=793
PRIMER_PAIR_17_PENALTY=8.366829
PRIMER_LEFT_17_PENALTY=1.995898
PRIMER_RIGHT_17_PENALTY=6.370931
PRIMER_LEFT_17_SEQUENCE=CACTTGAACCGGGTAGGTAAA
PRIMER_RIGHT_17_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_17=749,21
PRIMER_RIGHT_17=1520,22
PRIMER_LEFT_17_TM=59.004
PRIMER_RIGHT_17_TM=55.629
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=40.909
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.0000
PRIMER_RIGHT_17_END_STABILITY=6.1000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=772
PRIMER_PAIR_18_PENALTY=8.408355
PRIMER_LEFT_18_PENALTY=2.037424
PRIMER_RIGHT_18_PENALTY=6.370931
PRIMER_LEFT_18_SEQUENCE=ACCGAGGAGTAGTAAACCGCA
PRIMER_RIGHT_18_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_18=780,21
PRIMER_RIGHT_18=1520,22
PRIMER_LEFT_18_TM=61.037
PRIMER_RIGHT_18_TM=55.629
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=11.7000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=741
PRIMER_PAIR_19_PENALTY=8.412030
PRIMER_LEFT_19_PENALTY=2.041099
PRIMER_RIGHT_19_PENALTY=6.370931
PRIMER_LEFT_19_SEQUENCE=TTGGAACGGTAAATTTGGAGG
PRIMER_RIGHT_19_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_19=667,21
PRIMER_RIGHT_19=1520,22
PRIMER_LEFT_19_TM=61.041
PRIMER_RIGHT_19_TM=55.629
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.1000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=854
PRIMER_PAIR_20_PENALTY=8.466376
PRIMER_LEFT_20_PENALTY=2.095445
PRIMER_RIGHT_20_PENALTY=6.370931
PRIMER_LEFT_20_SEQUENCE=CGCAATTCTCCAACTAGATTCC
PRIMER_RIGHT_20_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_20=858,22
PRIMER_RIGHT_20=1520,22
PRIMER_LEFT_20_TM=60.095
PRIMER_RIGHT_20_TM=55.629
PRIMER_LEFT_20_GC_PERCENT=45.455
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=6.1000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=663
PRIMER_PAIR_21_PENALTY=8.517778
PRIMER_LEFT_21_PENALTY=2.146847
PRIMER_RIGHT_21_PENALTY=6.370931
PRIMER_LEFT_21_SEQUENCE=ACCCAATCCAAGCTTTCTCT
PRIMER_RIGHT_21_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_21=726,20
PRIMER_RIGHT_21=1520,22
PRIMER_LEFT_21_TM=57.853
PRIMER_RIGHT_21_TM=55.629
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=6.1000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=795
PRIMER_PAIR_22_PENALTY=8.578687
PRIMER_LEFT_22_PENALTY=2.207756
PRIMER_RIGHT_22_PENALTY=6.370931
PRIMER_LEFT_22_SEQUENCE=CCAATCCAAGCTTTCTCTCATC
PRIMER_RIGHT_22_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_22=728,22
PRIMER_RIGHT_22=1520,22
PRIMER_LEFT_22_TM=60.208
PRIMER_RIGHT_22_TM=55.629
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=793
PRIMER_PAIR_23_PENALTY=8.761838
PRIMER_LEFT_23_PENALTY=2.390906
PRIMER_RIGHT_23_PENALTY=6.370931
PRIMER_LEFT_23_SEQUENCE=TTAACCCAATCCAAGCTTTCTC
PRIMER_RIGHT_23_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_23=723,22
PRIMER_RIGHT_23=1520,22
PRIMER_LEFT_23_TM=59.609
PRIMER_RIGHT_23_TM=55.629
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=6.1000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=798
PRIMER_PAIR_24_PENALTY=8.762457
PRIMER_LEFT_24_PENALTY=2.391526
PRIMER_RIGHT_24_PENALTY=6.370931
PRIMER_LEFT_24_SEQUENCE=CACTTGAACCGGGTAGGTAA
PRIMER_RIGHT_24_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_24=749,20
PRIMER_RIGHT_24=1520,22
PRIMER_LEFT_24_TM=57.608
PRIMER_RIGHT_24_TM=55.629
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.2000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=772
PRIMER_PAIR_25_PENALTY=8.912187
PRIMER_LEFT_25_PENALTY=2.541256
PRIMER_RIGHT_25_PENALTY=6.370931
PRIMER_LEFT_25_SEQUENCE=GTTGGAACGGTAAATTTGGA
PRIMER_RIGHT_25_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_25=666,20
PRIMER_RIGHT_25=1520,22
PRIMER_LEFT_25_TM=57.459
PRIMER_RIGHT_25_TM=55.629
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=855
PRIMER_PAIR_26_PENALTY=8.920542
PRIMER_LEFT_26_PENALTY=2.549611
PRIMER_RIGHT_26_PENALTY=6.370931
PRIMER_LEFT_26_SEQUENCE=TGGAACGGTAAATTTGGAGGA
PRIMER_RIGHT_26_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_26=668,21
PRIMER_RIGHT_26=1520,22
PRIMER_LEFT_26_TM=61.550
PRIMER_RIGHT_26_TM=55.629
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=853
PRIMER_PAIR_27_PENALTY=8.946502
PRIMER_LEFT_27_PENALTY=2.575571
PRIMER_RIGHT_27_PENALTY=6.370931
PRIMER_LEFT_27_SEQUENCE=TGTTAGAAGAAACGTGCAGACC
PRIMER_RIGHT_27_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_27=640,22
PRIMER_RIGHT_27=1520,22
PRIMER_LEFT_27_TM=59.424
PRIMER_RIGHT_27_TM=55.629
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=6.1000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=881
PRIMER_PAIR_28_PENALTY=8.958013
PRIMER_LEFT_28_PENALTY=2.587081
PRIMER_RIGHT_28_PENALTY=6.370931
PRIMER_LEFT_28_SEQUENCE=CACCGGTCCGTAAAAATCATAG
PRIMER_RIGHT_28_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_28=692,22
PRIMER_RIGHT_28=1520,22
PRIMER_LEFT_28_TM=60.587
PRIMER_RIGHT_28_TM=55.629
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=5.9000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=829
PRIMER_PAIR_29_PENALTY=8.988816
PRIMER_LEFT_29_PENALTY=2.617885
PRIMER_RIGHT_29_PENALTY=6.370931
PRIMER_LEFT_29_SEQUENCE=CCCAATCCAAGCTTTCTCTCA
PRIMER_RIGHT_29_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_29=727,21
PRIMER_RIGHT_29=1520,22
PRIMER_LEFT_29_TM=61.618
PRIMER_RIGHT_29_TM=55.629
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.1000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=794
PRIMER_PAIR_30_PENALTY=9.055570
PRIMER_LEFT_30_PENALTY=2.684639
PRIMER_RIGHT_30_PENALTY=6.370931
PRIMER_LEFT_30_SEQUENCE=TAACCCAATCCAAGCTTTCTC
PRIMER_RIGHT_30_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_30=724,21
PRIMER_RIGHT_30=1520,22
PRIMER_LEFT_30_TM=58.315
PRIMER_RIGHT_30_TM=55.629
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.1000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=797
PRIMER_PAIR_31_PENALTY=9.107035
PRIMER_LEFT_31_PENALTY=2.736104
PRIMER_RIGHT_31_PENALTY=6.370931
PRIMER_LEFT_31_SEQUENCE=TAACCCAATCCAAGCTTTCTCT
PRIMER_RIGHT_31_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_31=724,22
PRIMER_RIGHT_31=1520,22
PRIMER_LEFT_31_TM=59.264
PRIMER_RIGHT_31_TM=55.629
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=6.1000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=797
PRIMER_PAIR_32_PENALTY=9.230041
PRIMER_LEFT_32_PENALTY=2.859110
PRIMER_RIGHT_32_PENALTY=6.370931
PRIMER_LEFT_32_SEQUENCE=CCGGTCCGTAAAAATCATAG
PRIMER_RIGHT_32_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_32=694,20
PRIMER_RIGHT_32=1520,22
PRIMER_LEFT_32_TM=57.141
PRIMER_RIGHT_32_TM=55.629
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=5.9000
PRIMER_RIGHT_32_END_STABILITY=6.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=827
PRIMER_PAIR_33_PENALTY=9.276606
PRIMER_LEFT_33_PENALTY=2.905675
PRIMER_RIGHT_33_PENALTY=6.370931
PRIMER_LEFT_33_SEQUENCE=GCAATTCTCCAACTAGATTCCC
PRIMER_RIGHT_33_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_33=859,22
PRIMER_RIGHT_33=1520,22
PRIMER_LEFT_33_TM=59.094
PRIMER_RIGHT_33_TM=55.629
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=40.909
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=6.1000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=662
PRIMER_PAIR_34_PENALTY=9.317279
PRIMER_LEFT_34_PENALTY=2.946348
PRIMER_RIGHT_34_PENALTY=6.370931
PRIMER_LEFT_34_SEQUENCE=AACCCAATCCAAGCTTTCTCTC
PRIMER_RIGHT_34_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_34=725,22
PRIMER_RIGHT_34=1520,22
PRIMER_LEFT_34_TM=60.946
PRIMER_RIGHT_34_TM=55.629
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.1000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=796
PRIMER_PAIR_35_PENALTY=9.357476
PRIMER_LEFT_35_PENALTY=2.986545
PRIMER_RIGHT_35_PENALTY=6.370931
PRIMER_LEFT_35_SEQUENCE=ACCGGTCCGTAAAAATCATAG
PRIMER_RIGHT_35_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_35=693,21
PRIMER_RIGHT_35=1520,22
PRIMER_LEFT_35_TM=58.013
PRIMER_RIGHT_35_TM=55.629
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=5.9000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=828
PRIMER_PAIR_36_PENALTY=9.626487
PRIMER_LEFT_36_PENALTY=3.255555
PRIMER_RIGHT_36_PENALTY=6.370931
PRIMER_LEFT_36_SEQUENCE=CAATCCAAGCTTTCTCTCATCAC
PRIMER_RIGHT_36_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_36=729,23
PRIMER_RIGHT_36=1520,22
PRIMER_LEFT_36_TM=60.256
PRIMER_RIGHT_36_TM=55.629
PRIMER_LEFT_36_GC_PERCENT=43.478
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.3000
PRIMER_RIGHT_36_END_STABILITY=6.1000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=792
PRIMER_PAIR_37_PENALTY=9.668428
PRIMER_LEFT_37_PENALTY=3.297497
PRIMER_RIGHT_37_PENALTY=6.370931
PRIMER_LEFT_37_SEQUENCE=GCTGCTCGCAATTCTCCAAC
PRIMER_RIGHT_37_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_37=852,20
PRIMER_RIGHT_37=1520,22
PRIMER_LEFT_37_TM=63.297
PRIMER_RIGHT_37_TM=55.629
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.1000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=669
PRIMER_PAIR_38_PENALTY=9.784531
PRIMER_LEFT_38_PENALTY=3.413600
PRIMER_RIGHT_38_PENALTY=6.370931
PRIMER_LEFT_38_SEQUENCE=TCTCATCACTTGAACCGGGTAG
PRIMER_RIGHT_38_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_38=743,22
PRIMER_RIGHT_38=1520,22
PRIMER_LEFT_38_TM=61.414
PRIMER_RIGHT_38_TM=55.629
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.1000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=778
PRIMER_PAIR_39_PENALTY=9.894984
PRIMER_LEFT_39_PENALTY=3.524053
PRIMER_RIGHT_39_PENALTY=6.370931
PRIMER_LEFT_39_SEQUENCE=CGGTCAACAGCAAAGTCGGT
PRIMER_RIGHT_39_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_39=802,20
PRIMER_RIGHT_39=1520,22
PRIMER_LEFT_39_TM=63.524
PRIMER_RIGHT_39_TM=55.629
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=6.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=719
PRIMER_PAIR_40_PENALTY=9.921582
PRIMER_LEFT_40_PENALTY=3.550651
PRIMER_RIGHT_40_PENALTY=6.370931
PRIMER_LEFT_40_SEQUENCE=TAACCCAATCCAAGCTTTCT
PRIMER_RIGHT_40_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_40=724,20
PRIMER_RIGHT_40=1520,22
PRIMER_LEFT_40_TM=56.449
PRIMER_RIGHT_40_TM=55.629
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.1000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=797
PRIMER_PAIR_41_PENALTY=10.056259
PRIMER_LEFT_41_PENALTY=3.685328
PRIMER_RIGHT_41_PENALTY=6.370931
PRIMER_LEFT_41_SEQUENCE=TTGTTAGAAGAAACGTGCAGACC
PRIMER_RIGHT_41_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_41=639,23
PRIMER_RIGHT_41=1520,22
PRIMER_LEFT_41_TM=60.685
PRIMER_RIGHT_41_TM=55.629
PRIMER_LEFT_41_GC_PERCENT=43.478
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=6.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=882
PRIMER_PAIR_42_PENALTY=10.113746
PRIMER_LEFT_42_PENALTY=3.742815
PRIMER_RIGHT_42_PENALTY=6.370931
PRIMER_LEFT_42_SEQUENCE=GTTGGAACGGTAAATTTGGAGG
PRIMER_RIGHT_42_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_42=666,22
PRIMER_RIGHT_42=1520,22
PRIMER_LEFT_42_TM=61.743
PRIMER_RIGHT_42_TM=55.629
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.4000
PRIMER_RIGHT_42_END_STABILITY=6.1000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=855
PRIMER_PAIR_43_PENALTY=10.126142
PRIMER_LEFT_43_PENALTY=3.755211
PRIMER_RIGHT_43_PENALTY=6.370931
PRIMER_LEFT_43_SEQUENCE=ACTTGAACCGGGTAGGTAAA
PRIMER_RIGHT_43_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_43=750,20
PRIMER_RIGHT_43=1520,22
PRIMER_LEFT_43_TM=56.245
PRIMER_RIGHT_43_TM=55.629
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.0000
PRIMER_RIGHT_43_END_STABILITY=6.1000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=771
PRIMER_PAIR_44_PENALTY=10.170419
PRIMER_LEFT_44_PENALTY=3.799488
PRIMER_RIGHT_44_PENALTY=6.370931
PRIMER_LEFT_44_SEQUENCE=TCCAAGCTTTCTCTCATCACTT
PRIMER_RIGHT_44_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_44=732,22
PRIMER_RIGHT_44=1520,22
PRIMER_LEFT_44_TM=58.201
PRIMER_RIGHT_44_TM=55.629
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.1000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=789
PRIMER_PAIR_45_PENALTY=10.170559
PRIMER_LEFT_45_PENALTY=3.799627
PRIMER_RIGHT_45_PENALTY=6.370931
PRIMER_LEFT_45_SEQUENCE=GCAGCGGTCAACAGCAAAGT
PRIMER_RIGHT_45_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_45=798,20
PRIMER_RIGHT_45=1520,22
PRIMER_LEFT_45_TM=63.800
PRIMER_RIGHT_45_TM=55.629
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.1000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=723
PRIMER_PAIR_46_PENALTY=10.183381
PRIMER_LEFT_46_PENALTY=3.812450
PRIMER_RIGHT_46_PENALTY=6.370931
PRIMER_LEFT_46_SEQUENCE=CAATTCTCCAACTAGATTCCCA
PRIMER_RIGHT_46_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_46=860,22
PRIMER_RIGHT_46=1520,22
PRIMER_LEFT_46_TM=58.188
PRIMER_RIGHT_46_TM=55.629
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=6.1000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=661
PRIMER_PAIR_47_PENALTY=10.235675
PRIMER_LEFT_47_PENALTY=3.864744
PRIMER_RIGHT_47_PENALTY=6.370931
PRIMER_LEFT_47_SEQUENCE=GAACGGTAAATTTGGAGGAC
PRIMER_RIGHT_47_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_47=670,20
PRIMER_RIGHT_47=1520,22
PRIMER_LEFT_47_TM=56.135
PRIMER_RIGHT_47_TM=55.629
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=6.1000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=851
PRIMER_PAIR_48_PENALTY=10.294594
PRIMER_LEFT_48_PENALTY=3.923662
PRIMER_RIGHT_48_PENALTY=6.370931
PRIMER_LEFT_48_SEQUENCE=TAACCCAATCCAAGCTTTCTCTC
PRIMER_RIGHT_48_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_48=724,23
PRIMER_RIGHT_48=1520,22
PRIMER_LEFT_48_TM=60.924
PRIMER_RIGHT_48_TM=55.629
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=6.1000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=797
PRIMER_PAIR_49_PENALTY=10.316050
PRIMER_LEFT_49_PENALTY=3.945119
PRIMER_RIGHT_49_PENALTY=6.370931
PRIMER_LEFT_49_SEQUENCE=GCTTTCTCTCATCACTTGAACC
PRIMER_RIGHT_49_SEQUENCE=GAGCTTCTTAATGAATGGTGTC
PRIMER_LEFT_49=737,22
PRIMER_RIGHT_49=1520,22
PRIMER_LEFT_49_TM=58.055
PRIMER_RIGHT_49_TM=55.629
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=6.1000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=784
=
SEQUENCE_ID=MAGIC.446_3_5588560_5588937_rsch-4_oy-0_92618_13884_TRUE
SEQUENCE_TARGET=950,477
SEQUENCE_TEMPLATE=NNNNNATATGTCTCTTCTATACCACCAANTTTGTCACGTGTGATATCTTCATTAAGTTTTAAGTTTTCTAATTTTTCTTGTTCTGTTTATNTNTGTTTTAAGTGTCTTTAAGTATNGNGTTTTGTGAAGTTGTTATGNCTGTNATATTANATGTGATTGGCTTTAATAAANGTGGGTGATTATTATACATATCAGTTGCACATTAGGAGAAAAGCNTATATTCATACTCAGTATCTCGGCATCTAGTGTATGATGNAAACAATTANCTTTAAGACTTTAGCATTATTTAAAGTGAGAAANANGAAANCTTCGNCATAATTGGAGAATTGGTCATNTTGATCAAAATCCAACACCAGACTACGCGCAGTCCGTTTCGAAGGAGGTACACTCCACTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTATTGTTGANAAAAACATGTATTATGTGAGCAAAGGAAAAAANNGNAAAAAAAACTANAGATCAGTAATATCAACTACTCCAATTCAACTNATTTGCTTCATATTCACAACATTCTNATATTTTAATGACTCGTGGATCACGAGATTATATTNACATTTCTATNGATTNATTAGTGGCCTACATTCAATGATATCAATAATTACACATTTTATCCAGAAATAAAAATNATCAATAAATACGCATGAAGGTCNTTGTAATGCATATCAATAACTCGTTCCCTCTCTCCTCAGGNAAATTCTCANTCTCTNGTCTGATNATAGTTTTGTAGAGTTTGGTTAANAAAACTAATCGTGTNCGNTACTANTACGTACAGNTAANTAATTAATATTCAAAAAGATGGCTCAAAAAGTGGAAGCAGGGGGTGNNGCCGGAGGAGCTTCATGGGATGATGGTNTCCACGATGGCGTTAGAAAGGTNCATGTAGGGCAAGGCCAAGATGGTGTGTCATCTATCAANGTCGTGTATGCGAAGGACTCTCAGGATNTTGAAGGANGTGAACANGGAAAGAAAACGTTACTTGGATTTGAAACGGTATGTAGTGGTGTTTGCTACCACTCTCTTTGAGTTTAACTTATTAGCCTTGTTCGGATCAGTTTTAAAGCTTTGTAAAATGAGAACCAATTAAGACTAGACCAATTCACACCTAATACGGTCTAGATTGAGTTAATCTNTACAACATCNNTACTCACGAGNGGTATCTTTTCGATCATTGCAGTNTGAGGTTGATGCAGACGACTACATAGTAGCAGTGCAAGTGACCTACGACAACGTGTTTGGCCAAGACTCTGACATCATCACATCTATTACCTTCAATACATTCAAGGGAAAAACATCTCCNCCCTATGGATTGGAAACGCAAAAGAAGTTTGTACTNAANGACAAAAACGGTGGNAAACTTNTTGGNTTCCATGGACGCGCTGGNGAAGCTNTATATGCTCTTGGAGCATATTTTGCTACAACCACAACTCCTGTGACTCCTGCCAAAAAACTATCTGCAATTGGTGGNGATGAAGGAACTGCATGGGACGANGGTGCGTACGATGGTGTCAAGAAGGTGTACGTNGGACAAGGCCAAGATGGGATATCAGCCGTTAAGTTTGAGTACAACAAAGGCGCGGAGAATATCGTAGGAGGTGAACATGGAAAGCCTACTTTACTCGGATTCGAGGAGGTATTNTTTNTNTTTNCTATCAAAATTCAAAATATGTTGTATCTTTTNATTTTCTTAAAACTAATTGANAGTNAGCAGATTGATCTATATGATTTNAAATCCTTTAATTGCAGTTTGAGATTGACTATCCAAGTGAATACATCACCGCAGTTGAAGGCACCTANGATAAAATCTTTGGGAGTGATGGCCTAATCATAACTATGCTTAGGTTCAAGACTAATAAACANACGTCTNCTCCCTTTGGACTTGAAGCTGGCACAGCCTTCGAACTTAAAGAGGAAGGNCACAAGATCGTTGGCTTCCATGGAAAAGCCAGNGANTTGCTTCATCAGTTTGGAGTCCATGTCATGCCANTCACCNANTGATCACATTNCAGCTACCTCCTTCGCTTCCATCGAACTTGTTAANGTGATCTCTTGAAAATAACTTGTTATACCTATGATGGTTATGCCTATCTATGTTTAAATGTTTTTAAGCGTTCTCTTTTGTGATGTTGGTCTTNATATAAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.078890
PRIMER_LEFT_0_PENALTY=1.026161
PRIMER_RIGHT_0_PENALTY=0.052729
PRIMER_LEFT_0_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_0_SEQUENCE=TTCTTTTGCGTTTCCAATCC
PRIMER_LEFT_0=906,20
PRIMER_RIGHT_0=1560,20
PRIMER_LEFT_0_TM=58.974
PRIMER_RIGHT_0_TM=60.053
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=655
PRIMER_PAIR_1_PENALTY=1.109564
PRIMER_LEFT_1_PENALTY=1.026161
PRIMER_RIGHT_1_PENALTY=0.083403
PRIMER_LEFT_1_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_1_SEQUENCE=ACGGCTGATATCCCATCTTG
PRIMER_LEFT_1=906,20
PRIMER_RIGHT_1=1788,20
PRIMER_LEFT_1_TM=58.974
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=883
PRIMER_PAIR_2_PENALTY=1.132471
PRIMER_LEFT_2_PENALTY=1.026161
PRIMER_RIGHT_2_PENALTY=0.106310
PRIMER_LEFT_2_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_2_SEQUENCE=TATCCCATCTTGGCCTTGTC
PRIMER_LEFT_2=906,20
PRIMER_RIGHT_2=1780,20
PRIMER_LEFT_2_TM=58.974
PRIMER_RIGHT_2_TM=59.894
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=875
PRIMER_PAIR_3_PENALTY=1.430281
PRIMER_LEFT_3_PENALTY=1.377552
PRIMER_RIGHT_3_PENALTY=0.052729
PRIMER_LEFT_3_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_3_SEQUENCE=TTCTTTTGCGTTTCCAATCC
PRIMER_LEFT_3=905,21
PRIMER_RIGHT_3=1560,20
PRIMER_LEFT_3_TM=60.378
PRIMER_RIGHT_3_TM=60.053
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=656
PRIMER_PAIR_4_PENALTY=1.460955
PRIMER_LEFT_4_PENALTY=1.377552
PRIMER_RIGHT_4_PENALTY=0.083403
PRIMER_LEFT_4_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_4_SEQUENCE=ACGGCTGATATCCCATCTTG
PRIMER_LEFT_4=905,21
PRIMER_RIGHT_4=1788,20
PRIMER_LEFT_4_TM=60.378
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=884
PRIMER_PAIR_5_PENALTY=1.483863
PRIMER_LEFT_5_PENALTY=1.377552
PRIMER_RIGHT_5_PENALTY=0.106310
PRIMER_LEFT_5_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_5_SEQUENCE=TATCCCATCTTGGCCTTGTC
PRIMER_LEFT_5=905,21
PRIMER_RIGHT_5=1780,20
PRIMER_LEFT_5_TM=60.378
PRIMER_RIGHT_5_TM=59.894
PRIMER_LEFT_5_GC_PERCENT=52.381
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=876
PRIMER_PAIR_6_PENALTY=1.682533
PRIMER_LEFT_6_PENALTY=1.026161
PRIMER_RIGHT_6_PENALTY=0.656372
PRIMER_LEFT_6_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_6_SEQUENCE=TGATATCCCATCTTGGCCTT
PRIMER_LEFT_6=906,20
PRIMER_RIGHT_6=1783,20
PRIMER_LEFT_6_TM=58.974
PRIMER_RIGHT_6_TM=59.344
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=878
PRIMER_PAIR_7_PENALTY=1.885373
PRIMER_LEFT_7_PENALTY=1.026161
PRIMER_RIGHT_7_PENALTY=0.859212
PRIMER_LEFT_7_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_7_SEQUENCE=GCTTTCCATGTTCACCTCCT
PRIMER_LEFT_7=906,20
PRIMER_RIGHT_7=1844,20
PRIMER_LEFT_7_TM=58.974
PRIMER_RIGHT_7_TM=59.141
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=939
PRIMER_PAIR_8_PENALTY=1.885373
PRIMER_LEFT_8_PENALTY=1.026161
PRIMER_RIGHT_8_PENALTY=0.859212
PRIMER_LEFT_8_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_8_SEQUENCE=AGGCTTTCCATGTTCACCTC
PRIMER_LEFT_8=906,20
PRIMER_RIGHT_8=1846,20
PRIMER_LEFT_8_TM=58.974
PRIMER_RIGHT_8_TM=59.141
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=941
PRIMER_PAIR_9_PENALTY=1.887552
PRIMER_LEFT_9_PENALTY=1.026161
PRIMER_RIGHT_9_PENALTY=0.861391
PRIMER_LEFT_9_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_9_SEQUENCE=GGCAGGAGTCACAGGAGTTG
PRIMER_LEFT_9=906,20
PRIMER_RIGHT_9=1678,20
PRIMER_LEFT_9_TM=58.974
PRIMER_RIGHT_9_TM=60.861
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=60.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=773
PRIMER_PAIR_10_PENALTY=1.975157
PRIMER_LEFT_10_PENALTY=1.026161
PRIMER_RIGHT_10_PENALTY=0.948996
PRIMER_LEFT_10_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_10_SEQUENCE=ATATTCTCCGCGCCTTTGTT
PRIMER_LEFT_10=906,20
PRIMER_RIGHT_10=1821,20
PRIMER_LEFT_10_TM=58.974
PRIMER_RIGHT_10_TM=60.949
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=2.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=916
PRIMER_PAIR_11_PENALTY=2.033925
PRIMER_LEFT_11_PENALTY=1.377552
PRIMER_RIGHT_11_PENALTY=0.656372
PRIMER_LEFT_11_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_11_SEQUENCE=TGATATCCCATCTTGGCCTT
PRIMER_LEFT_11=905,21
PRIMER_RIGHT_11=1783,20
PRIMER_LEFT_11_TM=60.378
PRIMER_RIGHT_11_TM=59.344
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=879
PRIMER_PAIR_12_PENALTY=2.192710
PRIMER_LEFT_12_PENALTY=1.026161
PRIMER_RIGHT_12_PENALTY=1.166549
PRIMER_LEFT_12_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_12_SEQUENCE=ATATCCCATCTTGGCCTTGTC
PRIMER_LEFT_12=906,20
PRIMER_RIGHT_12=1781,21
PRIMER_LEFT_12_TM=58.974
PRIMER_RIGHT_12_TM=60.167
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=2.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=876
PRIMER_PAIR_13_PENALTY=2.192710
PRIMER_LEFT_13_PENALTY=1.026161
PRIMER_RIGHT_13_PENALTY=1.166549
PRIMER_LEFT_13_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_13_SEQUENCE=GATATCCCATCTTGGCCTTGT
PRIMER_LEFT_13=906,20
PRIMER_RIGHT_13=1782,21
PRIMER_LEFT_13_TM=58.974
PRIMER_RIGHT_13_TM=60.167
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=877
PRIMER_PAIR_14_PENALTY=2.230062
PRIMER_LEFT_14_PENALTY=1.026161
PRIMER_RIGHT_14_PENALTY=1.203901
PRIMER_LEFT_14_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_14_SEQUENCE=TTTTTGGCAGGAGTCACAGG
PRIMER_LEFT_14=906,20
PRIMER_RIGHT_14=1683,20
PRIMER_LEFT_14_TM=58.974
PRIMER_RIGHT_14_TM=61.204
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=778
PRIMER_PAIR_15_PENALTY=2.236765
PRIMER_LEFT_15_PENALTY=1.377552
PRIMER_RIGHT_15_PENALTY=0.859212
PRIMER_LEFT_15_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_15_SEQUENCE=GCTTTCCATGTTCACCTCCT
PRIMER_LEFT_15=905,21
PRIMER_RIGHT_15=1844,20
PRIMER_LEFT_15_TM=60.378
PRIMER_RIGHT_15_TM=59.141
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=940
PRIMER_PAIR_16_PENALTY=2.236765
PRIMER_LEFT_16_PENALTY=1.377552
PRIMER_RIGHT_16_PENALTY=0.859212
PRIMER_LEFT_16_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_16_SEQUENCE=AGGCTTTCCATGTTCACCTC
PRIMER_LEFT_16=905,21
PRIMER_RIGHT_16=1846,20
PRIMER_LEFT_16_TM=60.378
PRIMER_RIGHT_16_TM=59.141
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=942
PRIMER_PAIR_17_PENALTY=2.238944
PRIMER_LEFT_17_PENALTY=1.377552
PRIMER_RIGHT_17_PENALTY=0.861391
PRIMER_LEFT_17_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_17_SEQUENCE=GGCAGGAGTCACAGGAGTTG
PRIMER_LEFT_17=905,21
PRIMER_RIGHT_17=1678,20
PRIMER_LEFT_17_TM=60.378
PRIMER_RIGHT_17_TM=60.861
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=60.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=774
PRIMER_PAIR_18_PENALTY=2.318845
PRIMER_LEFT_18_PENALTY=1.026161
PRIMER_RIGHT_18_PENALTY=1.292684
PRIMER_LEFT_18_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_18_SEQUENCE=CTGATATCCCATCTTGGCCTT
PRIMER_LEFT_18=906,20
PRIMER_RIGHT_18=1784,21
PRIMER_LEFT_18_TM=58.974
PRIMER_RIGHT_18_TM=60.293
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=879
PRIMER_PAIR_19_PENALTY=2.326548
PRIMER_LEFT_19_PENALTY=1.377552
PRIMER_RIGHT_19_PENALTY=0.948996
PRIMER_LEFT_19_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_19_SEQUENCE=ATATTCTCCGCGCCTTTGTT
PRIMER_LEFT_19=905,21
PRIMER_RIGHT_19=1821,20
PRIMER_LEFT_19_TM=60.378
PRIMER_RIGHT_19_TM=60.949
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=917
PRIMER_PAIR_20_PENALTY=2.416101
PRIMER_LEFT_20_PENALTY=1.026161
PRIMER_RIGHT_20_PENALTY=1.389940
PRIMER_LEFT_20_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_20_SEQUENCE=TTTGGCAGGAGTCACAGGAG
PRIMER_LEFT_20=906,20
PRIMER_RIGHT_20=1681,20
PRIMER_LEFT_20_TM=58.974
PRIMER_RIGHT_20_TM=61.390
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=776
PRIMER_PAIR_21_PENALTY=2.426727
PRIMER_LEFT_21_PENALTY=2.373998
PRIMER_RIGHT_21_PENALTY=0.052729
PRIMER_LEFT_21_SEQUENCE=TAACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_21_SEQUENCE=TTCTTTTGCGTTTCCAATCC
PRIMER_LEFT_21=904,22
PRIMER_RIGHT_21=1560,20
PRIMER_LEFT_21_TM=60.374
PRIMER_RIGHT_21_TM=60.053
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=657
PRIMER_PAIR_22_PENALTY=2.457401
PRIMER_LEFT_22_PENALTY=2.373998
PRIMER_RIGHT_22_PENALTY=0.083403
PRIMER_LEFT_22_SEQUENCE=TAACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_22_SEQUENCE=ACGGCTGATATCCCATCTTG
PRIMER_LEFT_22=904,22
PRIMER_RIGHT_22=1788,20
PRIMER_LEFT_22_TM=60.374
PRIMER_RIGHT_22_TM=59.917
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=885
PRIMER_PAIR_23_PENALTY=2.476321
PRIMER_LEFT_23_PENALTY=1.026161
PRIMER_RIGHT_23_PENALTY=1.450159
PRIMER_LEFT_23_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_23_SEQUENCE=CCAAACACGTTGTCGTAGGT
PRIMER_LEFT_23=906,20
PRIMER_RIGHT_23=1473,20
PRIMER_LEFT_23_TM=58.974
PRIMER_RIGHT_23_TM=58.550
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=568
PRIMER_PAIR_24_PENALTY=2.480308
PRIMER_LEFT_24_PENALTY=2.373998
PRIMER_RIGHT_24_PENALTY=0.106310
PRIMER_LEFT_24_SEQUENCE=TAACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_24_SEQUENCE=TATCCCATCTTGGCCTTGTC
PRIMER_LEFT_24=904,22
PRIMER_RIGHT_24=1780,20
PRIMER_LEFT_24_TM=60.374
PRIMER_RIGHT_24_TM=59.894
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=877
PRIMER_PAIR_25_PENALTY=2.487663
PRIMER_LEFT_25_PENALTY=1.026161
PRIMER_RIGHT_25_PENALTY=1.461502
PRIMER_LEFT_25_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_25_SEQUENCE=GATATTCTCCGCGCCTTTGT
PRIMER_LEFT_25=906,20
PRIMER_RIGHT_25=1822,20
PRIMER_LEFT_25_TM=58.974
PRIMER_RIGHT_25_TM=61.462
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=917
PRIMER_PAIR_26_PENALTY=2.544102
PRIMER_LEFT_26_PENALTY=1.377552
PRIMER_RIGHT_26_PENALTY=1.166549
PRIMER_LEFT_26_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_26_SEQUENCE=ATATCCCATCTTGGCCTTGTC
PRIMER_LEFT_26=905,21
PRIMER_RIGHT_26=1781,21
PRIMER_LEFT_26_TM=60.378
PRIMER_RIGHT_26_TM=60.167
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=877
PRIMER_PAIR_27_PENALTY=2.544102
PRIMER_LEFT_27_PENALTY=1.377552
PRIMER_RIGHT_27_PENALTY=1.166549
PRIMER_LEFT_27_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_27_SEQUENCE=GATATCCCATCTTGGCCTTGT
PRIMER_LEFT_27=905,21
PRIMER_RIGHT_27=1782,21
PRIMER_LEFT_27_TM=60.378
PRIMER_RIGHT_27_TM=60.167
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=878
PRIMER_PAIR_28_PENALTY=2.544514
PRIMER_LEFT_28_PENALTY=1.026161
PRIMER_RIGHT_28_PENALTY=1.518353
PRIMER_LEFT_28_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_28_SEQUENCE=GGCCAAACACGTTGTCGTAG
PRIMER_LEFT_28=906,20
PRIMER_RIGHT_28=1475,20
PRIMER_LEFT_28_TM=58.974
PRIMER_RIGHT_28_TM=61.518
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=570
PRIMER_PAIR_29_PENALTY=2.546771
PRIMER_LEFT_29_PENALTY=1.026161
PRIMER_RIGHT_29_PENALTY=1.520610
PRIMER_LEFT_29_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_29_SEQUENCE=AACGGCTGATATCCCATCTT
PRIMER_LEFT_29=906,20
PRIMER_RIGHT_29=1789,20
PRIMER_LEFT_29_TM=58.974
PRIMER_RIGHT_29_TM=58.479
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=884
PRIMER_PAIR_30_PENALTY=2.562462
PRIMER_LEFT_30_PENALTY=2.509733
PRIMER_RIGHT_30_PENALTY=0.052729
PRIMER_LEFT_30_SEQUENCE=AACTCGTTCCCTCTCTCCTC
PRIMER_RIGHT_30_SEQUENCE=TTCTTTTGCGTTTCCAATCC
PRIMER_LEFT_30=905,20
PRIMER_RIGHT_30=1560,20
PRIMER_LEFT_30_TM=57.490
PRIMER_RIGHT_30_TM=60.053
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=656
PRIMER_PAIR_31_PENALTY=2.581454
PRIMER_LEFT_31_PENALTY=1.377552
PRIMER_RIGHT_31_PENALTY=1.203901
PRIMER_LEFT_31_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_31_SEQUENCE=TTTTTGGCAGGAGTCACAGG
PRIMER_LEFT_31=905,21
PRIMER_RIGHT_31=1683,20
PRIMER_LEFT_31_TM=60.378
PRIMER_RIGHT_31_TM=61.204
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=779
PRIMER_PAIR_32_PENALTY=2.593136
PRIMER_LEFT_32_PENALTY=2.509733
PRIMER_RIGHT_32_PENALTY=0.083403
PRIMER_LEFT_32_SEQUENCE=AACTCGTTCCCTCTCTCCTC
PRIMER_RIGHT_32_SEQUENCE=ACGGCTGATATCCCATCTTG
PRIMER_LEFT_32=905,20
PRIMER_RIGHT_32=1788,20
PRIMER_LEFT_32_TM=57.490
PRIMER_RIGHT_32_TM=59.917
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=884
PRIMER_PAIR_33_PENALTY=2.616043
PRIMER_LEFT_33_PENALTY=2.509733
PRIMER_RIGHT_33_PENALTY=0.106310
PRIMER_LEFT_33_SEQUENCE=AACTCGTTCCCTCTCTCCTC
PRIMER_RIGHT_33_SEQUENCE=TATCCCATCTTGGCCTTGTC
PRIMER_LEFT_33=905,20
PRIMER_RIGHT_33=1780,20
PRIMER_LEFT_33_TM=57.490
PRIMER_RIGHT_33_TM=59.894
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=2.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=876
PRIMER_PAIR_34_PENALTY=2.620433
PRIMER_LEFT_34_PENALTY=1.026161
PRIMER_RIGHT_34_PENALTY=1.594272
PRIMER_LEFT_34_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_34_SEQUENCE=CTACGATATTCTCCGCGCCT
PRIMER_LEFT_34=906,20
PRIMER_RIGHT_34=1826,20
PRIMER_LEFT_34_TM=58.974
PRIMER_RIGHT_34_TM=61.594
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=11.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=921
PRIMER_PAIR_35_PENALTY=2.670236
PRIMER_LEFT_35_PENALTY=1.377552
PRIMER_RIGHT_35_PENALTY=1.292684
PRIMER_LEFT_35_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_35_SEQUENCE=CTGATATCCCATCTTGGCCTT
PRIMER_LEFT_35=905,21
PRIMER_RIGHT_35=1784,21
PRIMER_LEFT_35_TM=60.378
PRIMER_RIGHT_35_TM=60.293
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=880
PRIMER_PAIR_36_PENALTY=2.692247
PRIMER_LEFT_36_PENALTY=1.026161
PRIMER_RIGHT_36_PENALTY=1.666086
PRIMER_LEFT_36_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_36_SEQUENCE=ATATCCCATCTTGGCCTTGT
PRIMER_LEFT_36=906,20
PRIMER_RIGHT_36=1781,20
PRIMER_LEFT_36_TM=58.974
PRIMER_RIGHT_36_TM=58.334
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=2.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=876
PRIMER_PAIR_37_PENALTY=2.767493
PRIMER_LEFT_37_PENALTY=1.377552
PRIMER_RIGHT_37_PENALTY=1.389940
PRIMER_LEFT_37_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_37_SEQUENCE=TTTGGCAGGAGTCACAGGAG
PRIMER_LEFT_37=905,21
PRIMER_RIGHT_37=1681,20
PRIMER_LEFT_37_TM=60.378
PRIMER_RIGHT_37_TM=61.390
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=777
PRIMER_PAIR_38_PENALTY=2.778208
PRIMER_LEFT_38_PENALTY=1.026161
PRIMER_RIGHT_38_PENALTY=1.752047
PRIMER_LEFT_38_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_38_SEQUENCE=CAGGAGTCACAGGAGTTGTGG
PRIMER_LEFT_38=906,20
PRIMER_RIGHT_38=1676,21
PRIMER_LEFT_38_TM=58.974
PRIMER_RIGHT_38_TM=60.752
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=57.143
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=771
PRIMER_PAIR_39_PENALTY=2.802926
PRIMER_LEFT_39_PENALTY=1.026161
PRIMER_RIGHT_39_PENALTY=1.776765
PRIMER_LEFT_39_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_39_SEQUENCE=TTTTGGCAGGAGTCACAGGA
PRIMER_LEFT_39=906,20
PRIMER_RIGHT_39=1682,20
PRIMER_LEFT_39_TM=58.974
PRIMER_RIGHT_39_TM=61.777
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=777
PRIMER_PAIR_40_PENALTY=2.816390
PRIMER_LEFT_40_PENALTY=2.763661
PRIMER_RIGHT_40_PENALTY=0.052729
PRIMER_LEFT_40_SEQUENCE=CAATAACTCGTTCCCTCTCTCC
PRIMER_RIGHT_40_SEQUENCE=TTCTTTTGCGTTTCCAATCC
PRIMER_LEFT_40=901,22
PRIMER_RIGHT_40=1560,20
PRIMER_LEFT_40_TM=59.236
PRIMER_RIGHT_40_TM=60.053
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=660
PRIMER_PAIR_41_PENALTY=2.827034
PRIMER_LEFT_41_PENALTY=1.026161
PRIMER_RIGHT_41_PENALTY=1.800873
PRIMER_LEFT_41_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_41_SEQUENCE=GGCTTTCCATGTTCACCTCC
PRIMER_LEFT_41=906,20
PRIMER_RIGHT_41=1845,20
PRIMER_LEFT_41_TM=58.974
PRIMER_RIGHT_41_TM=61.801
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=940
PRIMER_PAIR_42_PENALTY=2.827712
PRIMER_LEFT_42_PENALTY=1.377552
PRIMER_RIGHT_42_PENALTY=1.450159
PRIMER_LEFT_42_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_42_SEQUENCE=CCAAACACGTTGTCGTAGGT
PRIMER_LEFT_42=905,21
PRIMER_RIGHT_42=1473,20
PRIMER_LEFT_42_TM=60.378
PRIMER_RIGHT_42_TM=58.550
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=569
PRIMER_PAIR_43_PENALTY=2.839054
PRIMER_LEFT_43_PENALTY=1.377552
PRIMER_RIGHT_43_PENALTY=1.461502
PRIMER_LEFT_43_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_43_SEQUENCE=GATATTCTCCGCGCCTTTGT
PRIMER_LEFT_43=905,21
PRIMER_RIGHT_43=1822,20
PRIMER_LEFT_43_TM=60.378
PRIMER_RIGHT_43_TM=61.462
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=918
PRIMER_PAIR_44_PENALTY=2.841765
PRIMER_LEFT_44_PENALTY=1.026161
PRIMER_RIGHT_44_PENALTY=1.815604
PRIMER_LEFT_44_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_44_SEQUENCE=TAGGCTTTCCATGTTCACCTC
PRIMER_LEFT_44=906,20
PRIMER_RIGHT_44=1847,21
PRIMER_LEFT_44_TM=58.974
PRIMER_RIGHT_44_TM=59.184
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=942
PRIMER_PAIR_45_PENALTY=2.847064
PRIMER_LEFT_45_PENALTY=2.763661
PRIMER_RIGHT_45_PENALTY=0.083403
PRIMER_LEFT_45_SEQUENCE=CAATAACTCGTTCCCTCTCTCC
PRIMER_RIGHT_45_SEQUENCE=ACGGCTGATATCCCATCTTG
PRIMER_LEFT_45=901,22
PRIMER_RIGHT_45=1788,20
PRIMER_LEFT_45_TM=59.236
PRIMER_RIGHT_45_TM=59.917
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=888
PRIMER_PAIR_46_PENALTY=2.869972
PRIMER_LEFT_46_PENALTY=2.763661
PRIMER_RIGHT_46_PENALTY=0.106310
PRIMER_LEFT_46_SEQUENCE=CAATAACTCGTTCCCTCTCTCC
PRIMER_RIGHT_46_SEQUENCE=TATCCCATCTTGGCCTTGTC
PRIMER_LEFT_46=901,22
PRIMER_RIGHT_46=1780,20
PRIMER_LEFT_46_TM=59.236
PRIMER_RIGHT_46_TM=59.894
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=880
PRIMER_PAIR_47_PENALTY=2.895905
PRIMER_LEFT_47_PENALTY=1.377552
PRIMER_RIGHT_47_PENALTY=1.518353
PRIMER_LEFT_47_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_47_SEQUENCE=GGCCAAACACGTTGTCGTAG
PRIMER_LEFT_47=905,21
PRIMER_RIGHT_47=1475,20
PRIMER_LEFT_47_TM=60.378
PRIMER_RIGHT_47_TM=61.518
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=571
PRIMER_PAIR_48_PENALTY=2.898163
PRIMER_LEFT_48_PENALTY=1.377552
PRIMER_RIGHT_48_PENALTY=1.520610
PRIMER_LEFT_48_SEQUENCE=AACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_48_SEQUENCE=AACGGCTGATATCCCATCTT
PRIMER_LEFT_48=905,21
PRIMER_RIGHT_48=1789,20
PRIMER_LEFT_48_TM=60.378
PRIMER_RIGHT_48_TM=58.479
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.6000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=885
PRIMER_PAIR_49_PENALTY=2.962098
PRIMER_LEFT_49_PENALTY=1.026161
PRIMER_RIGHT_49_PENALTY=1.935937
PRIMER_LEFT_49_SEQUENCE=ACTCGTTCCCTCTCTCCTCA
PRIMER_RIGHT_49_SEQUENCE=GGAGTCACAGGAGTTGTGGTT
PRIMER_LEFT_49=906,20
PRIMER_RIGHT_49=1674,21
PRIMER_LEFT_49_TM=58.974
PRIMER_RIGHT_49_TM=59.064
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=769
=
SEQUENCE_ID=MAGIC.433_4_4327039_4327060_sf-2_edi-0_6116_8935_TRUE
SEQUENCE_TARGET=950,121
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_1131740_1132120_tsu-0_ler-0_126835_38873_TRUE
SEQUENCE_TARGET=950,480
SEQUENCE_TEMPLATE=TAAAAATGANATTAAACTGTATAGAAATTATTGCGTGTTTATGTTTCTATGTGTGTGCGTGTGTGATCACACCGTGCATGTATGGTTTCGTGTTTAATTTGTATATAGATGTATCTGTATCACATATATTACATAAGTATAATGAAATAGTGGCCTACGATTTTGGATTTTAAAGAAGATCTTATGTTCTAAGAAGGTAGGCATTGCATCAAGATTTATAGTTAAGAAAGAAAAAAAGTGAAAAGAAAGCATTTTANNAACATCACAAAGAAAAGCATCTTTTACATGAATTTGGCTGAAATGTAATCTACATTATTCTAAGTGAGTTGTATAACAAACTAATATGAGAACAAGTNAATCAAAATGGTGAACAAATTTAAAGAATGNATAAGAAACATGAGTAGAAATNNNAANANANATATATATANNTCTTTAATTAATTCTTCTAGATCATAATAAGATTCTATGTTCTTTTTATTTATTTCTTTCATATCGTTTATAAGTAAACAAAAGAGAGTATGTTATTTGATAATTTAGACAAGAAATGTATAATCAAGTTTAGATACTTTCATTCTTTNGTTTTTTGGGTTTAAAGGTTTTGTTGAACGGTTGTAAAATCACTATCGAACCCATCTGCTANTATAGAACGAGGAATATTCTCATGAAGTACTTTTATAAAAGCATTGAAGTTCATGCTTAAGCAAATTAAGCATANAAGTTAACCCATCAAAATCTACTACTTTATATGTCAGTAGTGTTCAATGTCGTATTCGTAATGCTTTACTATGAAAAATGGCTTANACTCACGGAAAAAGGAAGAAANATAAATAAAATCATAATTTAGGTTCTTTACGCATTACGTATTTTGTTATTGTCTAGTGTTTTNTAGAGCATTNGTTTTTCAACAGTTCTACCTTTTTGTAAATGTGAGGTAATTTGAAGATATTTTAACTCGAAATTNAAAAATTCAACAATCTATCGTGAAATTTTTTACATAATTNATAGCNAAATATCCCACATACATATTAAAANNNNNNNAAAAAAAAAAAAAAAAACAATCGTGAAACTGTTTTAGTAGCCCATTGGGNCCAATACNAATGANGCCTAATTTATNAAAAAATCTAAAAGTACACCCACTGGCCCATTAATATCTGAAATGTCTTANTTTTTTTCTTTTTTTGGGAACAAAGAAATGTCTTACATTAATTTAGTGATNAAATTTTGGTAGTTACCTTGTCTGANATGTCTTTGATTTTAGAGATTTATCCACNGGTAAAACTTCAAAAAATAGTTTTTTCTTTTCTTTTCTTTTTTCCAAAAACTTCAAGAATATAGATTAAAGGCTCAATACCTCAAATTATCAATAATAAGGTCGTTTCGNGTTTTCATAAACGAGGAATGTCACAATTTAAAATTGGCTTATGGTTTGACGCTATGATAAATTAATAAGGGTCCATCAATTGTNNNNAAAAAAAAAAAAAAAAATGNCAATTGTGAAAGAATATATATACTATATTTTTAATAGTGAAAGAATATTAAACTTCTCTATGTCTCTCTANTAAATTTTAAATACAATGTACATACAAGTTTCACCCACTTCACCAACTAGAACTCAATTCCTTTATTATTATTCACATTTCAATGTCTGCNGAATACCTCAACAAACTTTCTTCTGTACTCTTCCCATCCTTCACATGTTAATGATCCGTTGCCACAGTAATGTATAGNGACAAGGATTTTTNCNANAAGTACAGAGACACATATCATTACAATAACATGCAACAGTNTTAATTCTTCGTGTATATAATATAGTCCGGCAAAGAAATAAATAAGAGTTAAACTGTGACTCCTCATANTTTAAAATAGACTTNTTCTTCTTTTNTTTTTTTTCAAATTAAAGGNNNNAAAAAAAAATCGGTTGCGTTTTAACTGGAGAGTTAAGTCATCTGCTGCCACTCTTAAATTTTCNCACATATATTCATTTTTCGCACGTGATTTCGCCATATCATCATAATATCGATAATTATTTTAGTAATCAATTTTCTTAAGNGCTGCGAGGGTTTATTACTCTTCTGAACCTAAATACAGAATACAGTACATTTAGAGCAAATAATTAATGGCTCATGGTTGACNGAAAACAATNATTATTGGCTCATAAGTCATATATACNCTTTAAATGATTNCTGNATTAATTGAGANGAAACCNTTTGTGTATGTATGTTATGCTTTTTGTTAGTGAAGTACTATCGTTTATAGCCATGAGTTTTCAGTTACCATGCATGCATTTTGGTTGGTTTTGATTAAACTGAATCAATTAATGTGGATTAGGNTTATCTCAATTTTGGTTTCAATCGAGNAGATCCTCAATTTCCAGANANAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.260167
PRIMER_LEFT_0_PENALTY=1.291527
PRIMER_RIGHT_0_PENALTY=1.968639
PRIMER_LEFT_0_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_0_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_0=617,21
PRIMER_RIGHT_0=1603,21
PRIMER_LEFT_0_TM=59.708
PRIMER_RIGHT_0_TM=60.969
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=987
PRIMER_PAIR_1_PENALTY=3.261726
PRIMER_LEFT_1_PENALTY=1.291527
PRIMER_RIGHT_1_PENALTY=1.970198
PRIMER_LEFT_1_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_1_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_1=617,21
PRIMER_RIGHT_1=1602,20
PRIMER_LEFT_1_TM=59.708
PRIMER_RIGHT_1_TM=58.030
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=986
PRIMER_PAIR_2_PENALTY=3.261726
PRIMER_LEFT_2_PENALTY=1.291527
PRIMER_RIGHT_2_PENALTY=1.970198
PRIMER_LEFT_2_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_2_SEQUENCE=TTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_2=617,21
PRIMER_RIGHT_2=1604,20
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=58.030
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=988
PRIMER_PAIR_3_PENALTY=3.261726
PRIMER_LEFT_3_PENALTY=1.291527
PRIMER_RIGHT_3_PENALTY=1.970198
PRIMER_LEFT_3_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_3_SEQUENCE=AGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_3=617,21
PRIMER_RIGHT_3=1606,20
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=58.030
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=990
PRIMER_PAIR_4_PENALTY=3.337400
PRIMER_LEFT_4_PENALTY=1.291527
PRIMER_RIGHT_4_PENALTY=2.045872
PRIMER_LEFT_4_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_4_SEQUENCE=GTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_4=617,21
PRIMER_RIGHT_4=1605,21
PRIMER_LEFT_4_TM=59.708
PRIMER_RIGHT_4_TM=58.954
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=989
PRIMER_PAIR_5_PENALTY=3.337400
PRIMER_LEFT_5_PENALTY=1.291527
PRIMER_RIGHT_5_PENALTY=2.045872
PRIMER_LEFT_5_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_5_SEQUENCE=AGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_5=617,21
PRIMER_RIGHT_5=1606,21
PRIMER_LEFT_5_TM=59.708
PRIMER_RIGHT_5_TM=58.954
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=990
PRIMER_PAIR_6_PENALTY=3.353488
PRIMER_LEFT_6_PENALTY=1.291527
PRIMER_RIGHT_6_PENALTY=2.061961
PRIMER_LEFT_6_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_6_SEQUENCE=AGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_6=617,21
PRIMER_RIGHT_6=1606,22
PRIMER_LEFT_6_TM=59.708
PRIMER_RIGHT_6_TM=59.938
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=990
PRIMER_PAIR_7_PENALTY=3.421470
PRIMER_LEFT_7_PENALTY=1.291527
PRIMER_RIGHT_7_PENALTY=2.129943
PRIMER_LEFT_7_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_7_SEQUENCE=GTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_7=617,21
PRIMER_RIGHT_7=1605,20
PRIMER_LEFT_7_TM=59.708
PRIMER_RIGHT_7_TM=57.870
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=989
PRIMER_PAIR_8_PENALTY=3.658324
PRIMER_LEFT_8_PENALTY=1.291527
PRIMER_RIGHT_8_PENALTY=2.366796
PRIMER_LEFT_8_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_8_SEQUENCE=TCTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_8=617,21
PRIMER_RIGHT_8=1609,22
PRIMER_LEFT_8_TM=59.708
PRIMER_RIGHT_8_TM=59.633
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=993
PRIMER_PAIR_9_PENALTY=3.658324
PRIMER_LEFT_9_PENALTY=1.291527
PRIMER_RIGHT_9_PENALTY=2.366796
PRIMER_LEFT_9_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_9_SEQUENCE=TTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_9=617,21
PRIMER_RIGHT_9=1610,22
PRIMER_LEFT_9_TM=59.708
PRIMER_RIGHT_9_TM=59.633
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=994
PRIMER_PAIR_10_PENALTY=3.978934
PRIMER_LEFT_10_PENALTY=2.010294
PRIMER_RIGHT_10_PENALTY=1.968639
PRIMER_LEFT_10_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_10_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_10=616,22
PRIMER_RIGHT_10=1603,21
PRIMER_LEFT_10_TM=59.990
PRIMER_RIGHT_10_TM=60.969
PRIMER_LEFT_10_GC_PERCENT=45.455
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=988
PRIMER_PAIR_11_PENALTY=3.980493
PRIMER_LEFT_11_PENALTY=2.010294
PRIMER_RIGHT_11_PENALTY=1.970198
PRIMER_LEFT_11_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_11_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_11=616,22
PRIMER_RIGHT_11=1602,20
PRIMER_LEFT_11_TM=59.990
PRIMER_RIGHT_11_TM=58.030
PRIMER_LEFT_11_GC_PERCENT=45.455
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=987
PRIMER_PAIR_12_PENALTY=3.980493
PRIMER_LEFT_12_PENALTY=2.010294
PRIMER_RIGHT_12_PENALTY=1.970198
PRIMER_LEFT_12_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_12_SEQUENCE=TTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_12=616,22
PRIMER_RIGHT_12=1604,20
PRIMER_LEFT_12_TM=59.990
PRIMER_RIGHT_12_TM=58.030
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=989
PRIMER_PAIR_13_PENALTY=3.980493
PRIMER_LEFT_13_PENALTY=2.010294
PRIMER_RIGHT_13_PENALTY=1.970198
PRIMER_LEFT_13_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_13_SEQUENCE=AGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_13=616,22
PRIMER_RIGHT_13=1606,20
PRIMER_LEFT_13_TM=59.990
PRIMER_RIGHT_13_TM=58.030
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=991
PRIMER_PAIR_14_PENALTY=4.056167
PRIMER_LEFT_14_PENALTY=2.010294
PRIMER_RIGHT_14_PENALTY=2.045872
PRIMER_LEFT_14_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_14_SEQUENCE=GTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_14=616,22
PRIMER_RIGHT_14=1605,21
PRIMER_LEFT_14_TM=59.990
PRIMER_RIGHT_14_TM=58.954
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=990
PRIMER_PAIR_15_PENALTY=4.056167
PRIMER_LEFT_15_PENALTY=2.010294
PRIMER_RIGHT_15_PENALTY=2.045872
PRIMER_LEFT_15_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_15_SEQUENCE=AGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_15=616,22
PRIMER_RIGHT_15=1606,21
PRIMER_LEFT_15_TM=59.990
PRIMER_RIGHT_15_TM=58.954
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=991
PRIMER_PAIR_16_PENALTY=4.072255
PRIMER_LEFT_16_PENALTY=2.010294
PRIMER_RIGHT_16_PENALTY=2.061961
PRIMER_LEFT_16_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_16_SEQUENCE=AGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_16=616,22
PRIMER_RIGHT_16=1606,22
PRIMER_LEFT_16_TM=59.990
PRIMER_RIGHT_16_TM=59.938
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=991
PRIMER_PAIR_17_PENALTY=4.090515
PRIMER_LEFT_17_PENALTY=1.291527
PRIMER_RIGHT_17_PENALTY=2.798987
PRIMER_LEFT_17_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_17_SEQUENCE=TCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_17=617,21
PRIMER_RIGHT_17=1609,21
PRIMER_LEFT_17_TM=59.708
PRIMER_RIGHT_17_TM=58.201
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=993
PRIMER_PAIR_18_PENALTY=4.140237
PRIMER_LEFT_18_PENALTY=2.010294
PRIMER_RIGHT_18_PENALTY=2.129943
PRIMER_LEFT_18_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_18_SEQUENCE=GTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_18=616,22
PRIMER_RIGHT_18=1605,20
PRIMER_LEFT_18_TM=59.990
PRIMER_RIGHT_18_TM=57.870
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=990
PRIMER_PAIR_19_PENALTY=4.149574
PRIMER_LEFT_19_PENALTY=1.291527
PRIMER_RIGHT_19_PENALTY=2.858047
PRIMER_LEFT_19_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_19_SEQUENCE=CTAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_19=617,21
PRIMER_RIGHT_19=1608,22
PRIMER_LEFT_19_TM=59.708
PRIMER_RIGHT_19_TM=59.142
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=992
PRIMER_PAIR_20_PENALTY=4.172996
PRIMER_LEFT_20_PENALTY=1.291527
PRIMER_RIGHT_20_PENALTY=2.881468
PRIMER_LEFT_20_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_20_SEQUENCE=TAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_20=617,21
PRIMER_RIGHT_20=1607,21
PRIMER_LEFT_20_TM=59.708
PRIMER_RIGHT_20_TM=58.119
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=991
PRIMER_PAIR_21_PENALTY=4.202327
PRIMER_LEFT_21_PENALTY=2.233687
PRIMER_RIGHT_21_PENALTY=1.968639
PRIMER_LEFT_21_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_21_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_21=618,20
PRIMER_RIGHT_21=1603,21
PRIMER_LEFT_21_TM=57.766
PRIMER_RIGHT_21_TM=60.969
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=986
PRIMER_PAIR_22_PENALTY=4.203886
PRIMER_LEFT_22_PENALTY=2.233687
PRIMER_RIGHT_22_PENALTY=1.970198
PRIMER_LEFT_22_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_22_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_22=618,20
PRIMER_RIGHT_22=1602,20
PRIMER_LEFT_22_TM=57.766
PRIMER_RIGHT_22_TM=58.030
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=985
PRIMER_PAIR_23_PENALTY=4.203886
PRIMER_LEFT_23_PENALTY=2.233687
PRIMER_RIGHT_23_PENALTY=1.970198
PRIMER_LEFT_23_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_23_SEQUENCE=TTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_23=618,20
PRIMER_RIGHT_23=1604,20
PRIMER_LEFT_23_TM=57.766
PRIMER_RIGHT_23_TM=58.030
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=987
PRIMER_PAIR_24_PENALTY=4.203886
PRIMER_LEFT_24_PENALTY=2.233687
PRIMER_RIGHT_24_PENALTY=1.970198
PRIMER_LEFT_24_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_24_SEQUENCE=AGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_24=618,20
PRIMER_RIGHT_24=1606,20
PRIMER_LEFT_24_TM=57.766
PRIMER_RIGHT_24_TM=58.030
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=989
PRIMER_PAIR_25_PENALTY=4.279560
PRIMER_LEFT_25_PENALTY=2.233687
PRIMER_RIGHT_25_PENALTY=2.045872
PRIMER_LEFT_25_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_25_SEQUENCE=GTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_25=618,20
PRIMER_RIGHT_25=1605,21
PRIMER_LEFT_25_TM=57.766
PRIMER_RIGHT_25_TM=58.954
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=988
PRIMER_PAIR_26_PENALTY=4.279560
PRIMER_LEFT_26_PENALTY=2.233687
PRIMER_RIGHT_26_PENALTY=2.045872
PRIMER_LEFT_26_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_26_SEQUENCE=AGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_26=618,20
PRIMER_RIGHT_26=1606,21
PRIMER_LEFT_26_TM=57.766
PRIMER_RIGHT_26_TM=58.954
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=989
PRIMER_PAIR_27_PENALTY=4.287105
PRIMER_LEFT_27_PENALTY=1.291527
PRIMER_RIGHT_27_PENALTY=2.995578
PRIMER_LEFT_27_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_27_SEQUENCE=TAGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_27=617,21
PRIMER_RIGHT_27=1607,22
PRIMER_LEFT_27_TM=59.708
PRIMER_RIGHT_27_TM=59.004
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.455
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=991
PRIMER_PAIR_28_PENALTY=4.295648
PRIMER_LEFT_28_PENALTY=2.233687
PRIMER_RIGHT_28_PENALTY=2.061961
PRIMER_LEFT_28_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_28_SEQUENCE=AGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_28=618,20
PRIMER_RIGHT_28=1606,22
PRIMER_LEFT_28_TM=57.766
PRIMER_RIGHT_28_TM=59.938
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=989
PRIMER_PAIR_29_PENALTY=4.341027
PRIMER_LEFT_29_PENALTY=1.291527
PRIMER_RIGHT_29_PENALTY=3.049500
PRIMER_LEFT_29_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_29_SEQUENCE=TAGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_29=617,21
PRIMER_RIGHT_29=1607,23
PRIMER_LEFT_29_TM=59.708
PRIMER_RIGHT_29_TM=59.950
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=43.478
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=991
PRIMER_PAIR_30_PENALTY=4.341027
PRIMER_LEFT_30_PENALTY=1.291527
PRIMER_RIGHT_30_PENALTY=3.049500
PRIMER_LEFT_30_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_30_SEQUENCE=CTAGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_30=617,21
PRIMER_RIGHT_30=1608,23
PRIMER_LEFT_30_TM=59.708
PRIMER_RIGHT_30_TM=59.950
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=47.826
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=992
PRIMER_PAIR_31_PENALTY=4.363630
PRIMER_LEFT_31_PENALTY=2.233687
PRIMER_RIGHT_31_PENALTY=2.129943
PRIMER_LEFT_31_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_31_SEQUENCE=GTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_31=618,20
PRIMER_RIGHT_31=1605,20
PRIMER_LEFT_31_TM=57.766
PRIMER_RIGHT_31_TM=57.870
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=988
PRIMER_PAIR_32_PENALTY=4.377091
PRIMER_LEFT_32_PENALTY=2.010294
PRIMER_RIGHT_32_PENALTY=2.366796
PRIMER_LEFT_32_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_32_SEQUENCE=TCTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_32=616,22
PRIMER_RIGHT_32=1609,22
PRIMER_LEFT_32_TM=59.990
PRIMER_RIGHT_32_TM=59.633
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=994
PRIMER_PAIR_33_PENALTY=4.377091
PRIMER_LEFT_33_PENALTY=2.010294
PRIMER_RIGHT_33_PENALTY=2.366796
PRIMER_LEFT_33_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_33_SEQUENCE=TTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_33=616,22
PRIMER_RIGHT_33=1610,22
PRIMER_LEFT_33_TM=59.990
PRIMER_RIGHT_33_TM=59.633
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=995
PRIMER_PAIR_34_PENALTY=4.533694
PRIMER_LEFT_34_PENALTY=2.929208
PRIMER_RIGHT_34_PENALTY=1.604487
PRIMER_LEFT_34_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_34_SEQUENCE=AACATGTGAAGGATGGGAAGA
PRIMER_LEFT_34=802,20
PRIMER_RIGHT_34=1698,21
PRIMER_LEFT_34_TM=57.071
PRIMER_RIGHT_34_TM=59.396
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=897
PRIMER_PAIR_35_PENALTY=4.574138
PRIMER_LEFT_35_PENALTY=1.291527
PRIMER_RIGHT_35_PENALTY=3.282611
PRIMER_LEFT_35_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_35_SEQUENCE=CTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_35=617,21
PRIMER_RIGHT_35=1608,21
PRIMER_LEFT_35_TM=59.708
PRIMER_RIGHT_35_TM=57.717
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=4.574138
PRIMER_LEFT_36_PENALTY=1.291527
PRIMER_RIGHT_36_PENALTY=3.282611
PRIMER_LEFT_36_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_36_SEQUENCE=TTCTAGTTGGTGAAGTGGGTG
PRIMER_LEFT_36=617,21
PRIMER_RIGHT_36=1610,21
PRIMER_LEFT_36_TM=59.708
PRIMER_RIGHT_36_TM=57.717
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=994
PRIMER_PAIR_37_PENALTY=4.600484
PRIMER_LEFT_37_PENALTY=2.233687
PRIMER_RIGHT_37_PENALTY=2.366796
PRIMER_LEFT_37_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_37_SEQUENCE=TCTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_37=618,20
PRIMER_RIGHT_37=1609,22
PRIMER_LEFT_37_TM=57.766
PRIMER_RIGHT_37_TM=59.633
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=992
PRIMER_PAIR_38_PENALTY=4.600484
PRIMER_LEFT_38_PENALTY=2.233687
PRIMER_RIGHT_38_PENALTY=2.366796
PRIMER_LEFT_38_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_38_SEQUENCE=TTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_38=618,20
PRIMER_RIGHT_38=1610,22
PRIMER_LEFT_38_TM=57.766
PRIMER_RIGHT_38_TM=59.633
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=993
PRIMER_PAIR_39_PENALTY=4.666191
PRIMER_LEFT_39_PENALTY=1.291527
PRIMER_RIGHT_39_PENALTY=3.374664
PRIMER_LEFT_39_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_39_SEQUENCE=GTTCTAGTTGGTGAAGTGGGTG
PRIMER_LEFT_39=617,21
PRIMER_RIGHT_39=1611,22
PRIMER_LEFT_39_TM=59.708
PRIMER_RIGHT_39_TM=58.625
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=9.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=995
PRIMER_PAIR_40_PENALTY=4.696593
PRIMER_LEFT_40_PENALTY=1.291527
PRIMER_RIGHT_40_PENALTY=3.405066
PRIMER_LEFT_40_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_40_SEQUENCE=AGTTCTAGTTGGTGAAGTGGGTG
PRIMER_LEFT_40=617,21
PRIMER_RIGHT_40=1612,23
PRIMER_LEFT_40_TM=59.708
PRIMER_RIGHT_40_TM=59.595
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=47.826
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=9.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=996
PRIMER_PAIR_41_PENALTY=4.731350
PRIMER_LEFT_41_PENALTY=1.291527
PRIMER_RIGHT_41_PENALTY=3.439823
PRIMER_LEFT_41_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_41_SEQUENCE=TAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_41=617,21
PRIMER_RIGHT_41=1607,20
PRIMER_LEFT_41_TM=59.708
PRIMER_RIGHT_41_TM=56.560
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=991
PRIMER_PAIR_42_PENALTY=4.733258
PRIMER_LEFT_42_PENALTY=1.291527
PRIMER_RIGHT_42_PENALTY=3.441731
PRIMER_LEFT_42_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_42_SEQUENCE=GTTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_42=617,21
PRIMER_RIGHT_42=1611,23
PRIMER_LEFT_42_TM=59.708
PRIMER_RIGHT_42_TM=60.442
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=47.826
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=995
PRIMER_PAIR_43_PENALTY=4.809282
PRIMER_LEFT_43_PENALTY=2.010294
PRIMER_RIGHT_43_PENALTY=2.798987
PRIMER_LEFT_43_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_43_SEQUENCE=TCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_43=616,22
PRIMER_RIGHT_43=1609,21
PRIMER_LEFT_43_TM=59.990
PRIMER_RIGHT_43_TM=58.201
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=994
PRIMER_PAIR_44_PENALTY=4.856779
PRIMER_LEFT_44_PENALTY=1.291527
PRIMER_RIGHT_44_PENALTY=3.565251
PRIMER_LEFT_44_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_44_SEQUENCE=TGAGTTCTAGTTGGTGAAGTGGG
PRIMER_LEFT_44=617,21
PRIMER_RIGHT_44=1614,23
PRIMER_LEFT_44_TM=59.708
PRIMER_RIGHT_44_TM=60.565
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=47.826
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=998
PRIMER_PAIR_45_PENALTY=4.857871
PRIMER_LEFT_45_PENALTY=2.929208
PRIMER_RIGHT_45_PENALTY=1.928663
PRIMER_LEFT_45_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_45_SEQUENCE=CATGTGAAGGATGGGAAGAG
PRIMER_LEFT_45=802,20
PRIMER_RIGHT_45=1696,20
PRIMER_LEFT_45_TM=57.071
PRIMER_RIGHT_45_TM=58.071
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=895
PRIMER_PAIR_46_PENALTY=4.868341
PRIMER_LEFT_46_PENALTY=2.010294
PRIMER_RIGHT_46_PENALTY=2.858047
PRIMER_LEFT_46_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_46_SEQUENCE=CTAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_46=616,22
PRIMER_RIGHT_46=1608,22
PRIMER_LEFT_46_TM=59.990
PRIMER_RIGHT_46_TM=59.142
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=993
PRIMER_PAIR_47_PENALTY=4.891763
PRIMER_LEFT_47_PENALTY=2.010294
PRIMER_RIGHT_47_PENALTY=2.881468
PRIMER_LEFT_47_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_47_SEQUENCE=TAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_47=616,22
PRIMER_RIGHT_47=1607,21
PRIMER_LEFT_47_TM=59.990
PRIMER_RIGHT_47_TM=58.119
PRIMER_LEFT_47_GC_PERCENT=45.455
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=992
PRIMER_PAIR_48_PENALTY=4.897847
PRIMER_LEFT_48_PENALTY=2.929208
PRIMER_RIGHT_48_PENALTY=1.968639
PRIMER_LEFT_48_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_48_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_48=802,20
PRIMER_RIGHT_48=1603,21
PRIMER_LEFT_48_TM=57.071
PRIMER_RIGHT_48_TM=60.969
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=802
PRIMER_PAIR_49_PENALTY=4.899406
PRIMER_LEFT_49_PENALTY=2.929208
PRIMER_RIGHT_49_PENALTY=1.970198
PRIMER_LEFT_49_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_49_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_49=802,20
PRIMER_RIGHT_49=1602,20
PRIMER_LEFT_49_TM=57.071
PRIMER_RIGHT_49_TM=58.030
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=801
=
SEQUENCE_ID=MAGIC.105_4_17287503_17287830_col-0_po-0_2674_13371_TRUE
SEQUENCE_TARGET=950,427
SEQUENCE_TEMPLATE=ATCTCCAGACACAGAAGAACTTGACCATCAAACTGAACAACCTAAGCTANGTACACTCAAGATCTNAACTCCAGATNCCAGATTGAACATTACTATTTACTCCAGCACAGAGGCAAGATNNNNNACCATCTATCNATANCTCTANNNACTGATTCTAAGTGACTNNNNNNNNNNNNNNNAANCGAAATTTAAAGCAAGAATNATCACCATTGATCNAGAAGGAGGTGGATCAAGAGTGATNGACCGCNCCGACATCTTGGCGATTTCACCAATAGCAGCTTCTCTAACCCCAGGCANATCACTAGTNAGNTCTTCATAAGCAGCAACAAAAGCCTCTTGCCAAAATCTAGGCAATTTGATCCGACGACTNTTAGTATACACNTCCCACATATGCTTCACCACTTTCTCNCTCTCNTCCATTTCCTAAAAAATACCACAAATTCCAAAACCTCAAAAACCAGACTACAATCAAATTACACACCAAATCAACCGAAATAGAAACAGAGAGAGTAAACAACCAGAACGTCGAGATTATCGTAGTTCGGAATCCCTAGATCTTCCGATAACCGGTTCAGATTCCCGGTAGATAACCGGAGAGTGACGGTGCCAGTAAACATCGACCAAAANGCCAAAAGCAAAATCGCAGCAAACGCCCAGAACTTATATCGANCTCTTCCAAACAAGACCGNGTCAGAGCTTTCCTTCTTCGAATTCGNCNNAGCTGTTCCCGTCGCCGTACCAGTCGTCGTCGTCGTCGGCAATGCATCGTCCTCCTTCATCTNGATCTCAAATACNAATTACNACTCAANANTCTTNCTTAGTGAGTGATNCTAACACCAAATCTGANCTCTCGTGAATCGATTTCAGTAGATTCAGTNCAATNGNGAAAGTGTCGGCTCGATTTNGTTAAATCTGANAGAGAACTNNNNAAAAAAAAAAANCAGTTCACTCTCTNCACAGTTCACACTACTGGGCCATTNTTAGCTAGTAATTGGCGATGNATTAGCCGTTGATCTTAAATTGTTAATGAAAAAAATACTAAAGCGTGCGTCAATNNTGACCGTTGATTTATAATTTGATAAAAGCCACACAAAAGTACAACTAAACGACNTCGTGTAANNNNNNNGAANCCCCAAANGCTGTCGTTTCANTTAATGANAACNTTATANNNATCATGCTAAGCTGCACCGTTTTNGCCGCGTACNTTATCACCGTCTCTTTGAAAAAGCGCTTCGATTTCTTCAAGTGATTTTCCTTTCGTCTCCGGCAAGAGGAANAAGAAGAAATTCCACGCCACTGCCGCAACTCCGGCGAACATAAAGAAAGCNCCACCGGTGGTTATCGCACTAGTCAACGACAAAAACGACATCGACACGGTGGCGTTCATTACTCTGTTCACCGCAACGCCGAGACTCGCTCCTTGTGCCCTAAGCTTCAACGGGAAAACCTCAGAACTGTAGACCCAAGTTATTGGGCCNAGCCCAATAGAGAAAAACGCCACGAAACTATAAGCCGCAACTATGCTCAGTACTAAAGCCCACGCTAATTTCCCGCCAGCATTTTGGGCCATTGTAAGCCCAAATCCCAACATTGTCAACGCAATGACCATTCCTCCAACGCTGGTCAACAAAAGCTTCCTTCGACCTACCTTGTCTAACAATAAAGTCGCAGTGAAAATAAACGTCGTTTTCATGATTCCGACACCGATNGTAACCANGAAAAGCTTGTCTTTAGTCGTGATTCCTGCTTTCTTAAAGATCCTCGGACCGTATAAAAGCACNGCTTCGATTCCGGAGGCGTGTTGGAAGAAATGAATCCCAAGAGCNGTTAAAAGAACACGTCTCACTGCAGGAGTTGGTCTTAGAATGAGCTCTTTCCACACTCCNTCACCATGAGTCTTCTTACCCTCCATTTTCACAACATCGTCTACGCATTTNGGGTCGATTCCCGCAGCNGCTTTGATGTCTTGAAAGCGGAGTTCNGCTTCTTCAGGGGAATTNGATACCAATTCCAATATCTCCTTGCCTTCCTTNAGACGGCCTTGCATAATCAACCACCGTGGAGATTCCGGCATTTTCAAGATCCCGAACGCTAGCACTAGGGANGGNACCGCGGCTATACCGAGCATGAGTCTCCAACCGATATGNATAGGTAACTTGGAGAAGAAGTAATTCACGANATAACCTAGTAAAATNCCTATACTGATACAAAGGTGAGGAAGAGAAGCTAAGAGTCCTCTATGTGAAGCNGTTGCGATCTCGGCNGAGTAAACCGGAGCAACCATCAGNGCAAAACCGACTCCGAGTCCAGCGGTGCATCTACCGGAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.055002
PRIMER_LEFT_0_PENALTY=0.010545
PRIMER_RIGHT_0_PENALTY=0.044457
PRIMER_LEFT_0_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_0_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_0=586,20
PRIMER_RIGHT_0=1528,20
PRIMER_LEFT_0_TM=59.989
PRIMER_RIGHT_0_TM=60.044
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=943
PRIMER_PAIR_1_PENALTY=0.086099
PRIMER_LEFT_1_PENALTY=0.041642
PRIMER_RIGHT_1_PENALTY=0.044457
PRIMER_LEFT_1_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_1_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_1=760,20
PRIMER_RIGHT_1=1528,20
PRIMER_LEFT_1_TM=60.042
PRIMER_RIGHT_1_TM=60.044
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=769
PRIMER_PAIR_2_PENALTY=0.121356
PRIMER_LEFT_2_PENALTY=0.041642
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_2_SEQUENCE=AAGCTTTTGTTGACCAGCGT
PRIMER_LEFT_2=760,20
PRIMER_RIGHT_2=1635,20
PRIMER_LEFT_2_TM=60.042
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=876
PRIMER_PAIR_3_PENALTY=0.155822
PRIMER_LEFT_3_PENALTY=0.111365
PRIMER_RIGHT_3_PENALTY=0.044457
PRIMER_LEFT_3_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_3_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_3=604,20
PRIMER_RIGHT_3=1528,20
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=60.044
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=925
PRIMER_PAIR_4_PENALTY=0.305240
PRIMER_LEFT_4_PENALTY=0.010545
PRIMER_RIGHT_4_PENALTY=0.294694
PRIMER_LEFT_4_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_4_SEQUENCE=CTGAGGTTTTCCCGTTGAAG
PRIMER_LEFT_4=586,20
PRIMER_RIGHT_4=1451,20
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=59.705
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=866
PRIMER_PAIR_5_PENALTY=0.333252
PRIMER_LEFT_5_PENALTY=0.010545
PRIMER_RIGHT_5_PENALTY=0.322707
PRIMER_LEFT_5_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_5_SEQUENCE=TTACAATGGCCCAAAATGCT
PRIMER_LEFT_5=586,20
PRIMER_RIGHT_5=1574,20
PRIMER_LEFT_5_TM=59.989
PRIMER_RIGHT_5_TM=60.323
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.9000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=989
PRIMER_PAIR_6_PENALTY=0.333252
PRIMER_LEFT_6_PENALTY=0.010545
PRIMER_RIGHT_6_PENALTY=0.322707
PRIMER_LEFT_6_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_6_SEQUENCE=GCTTACAATGGCCCAAAATG
PRIMER_LEFT_6=586,20
PRIMER_RIGHT_6=1576,20
PRIMER_LEFT_6_TM=59.989
PRIMER_RIGHT_6_TM=60.323
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=991
PRIMER_PAIR_7_PENALTY=0.364349
PRIMER_LEFT_7_PENALTY=0.041642
PRIMER_RIGHT_7_PENALTY=0.322707
PRIMER_LEFT_7_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_7_SEQUENCE=TTACAATGGCCCAAAATGCT
PRIMER_LEFT_7=760,20
PRIMER_RIGHT_7=1574,20
PRIMER_LEFT_7_TM=60.042
PRIMER_RIGHT_7_TM=60.323
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=815
PRIMER_PAIR_8_PENALTY=0.364349
PRIMER_LEFT_8_PENALTY=0.041642
PRIMER_RIGHT_8_PENALTY=0.322707
PRIMER_LEFT_8_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_8_SEQUENCE=GCTTACAATGGCCCAAAATG
PRIMER_LEFT_8=760,20
PRIMER_RIGHT_8=1576,20
PRIMER_LEFT_8_TM=60.042
PRIMER_RIGHT_8_TM=60.323
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=817
PRIMER_PAIR_9_PENALTY=0.406060
PRIMER_LEFT_9_PENALTY=0.111365
PRIMER_RIGHT_9_PENALTY=0.294694
PRIMER_LEFT_9_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_9_SEQUENCE=CTGAGGTTTTCCCGTTGAAG
PRIMER_LEFT_9=604,20
PRIMER_RIGHT_9=1451,20
PRIMER_LEFT_9_TM=60.111
PRIMER_RIGHT_9_TM=59.705
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=848
PRIMER_PAIR_10_PENALTY=0.428907
PRIMER_LEFT_10_PENALTY=0.384450
PRIMER_RIGHT_10_PENALTY=0.044457
PRIMER_LEFT_10_SEQUENCE=AAAGCAAAATCGCAGCAAAC
PRIMER_RIGHT_10_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_10=631,20
PRIMER_RIGHT_10=1528,20
PRIMER_LEFT_10_TM=60.384
PRIMER_RIGHT_10_TM=60.044
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=898
PRIMER_PAIR_11_PENALTY=0.434072
PRIMER_LEFT_11_PENALTY=0.111365
PRIMER_RIGHT_11_PENALTY=0.322707
PRIMER_LEFT_11_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_11_SEQUENCE=TTACAATGGCCCAAAATGCT
PRIMER_LEFT_11=604,20
PRIMER_RIGHT_11=1574,20
PRIMER_LEFT_11_TM=60.111
PRIMER_RIGHT_11_TM=60.323
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=971
PRIMER_PAIR_12_PENALTY=0.434072
PRIMER_LEFT_12_PENALTY=0.111365
PRIMER_RIGHT_12_PENALTY=0.322707
PRIMER_LEFT_12_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_12_SEQUENCE=GCTTACAATGGCCCAAAATG
PRIMER_LEFT_12=604,20
PRIMER_RIGHT_12=1576,20
PRIMER_LEFT_12_TM=60.111
PRIMER_RIGHT_12_TM=60.323
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=973
PRIMER_PAIR_13_PENALTY=0.442031
PRIMER_LEFT_13_PENALTY=0.010545
PRIMER_RIGHT_13_PENALTY=0.431486
PRIMER_LEFT_13_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_13_SEQUENCE=GTTCTGAGGTTTTCCCGTTG
PRIMER_LEFT_13=586,20
PRIMER_RIGHT_13=1454,20
PRIMER_LEFT_13_TM=59.989
PRIMER_RIGHT_13_TM=59.569
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=8.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=869
PRIMER_PAIR_14_PENALTY=0.472938
PRIMER_LEFT_14_PENALTY=0.428481
PRIMER_RIGHT_14_PENALTY=0.044457
PRIMER_LEFT_14_SEQUENCE=GTGCCAGTAAACATCGACCA
PRIMER_RIGHT_14_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_14=603,20
PRIMER_RIGHT_14=1528,20
PRIMER_LEFT_14_TM=59.572
PRIMER_RIGHT_14_TM=60.044
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=926
PRIMER_PAIR_15_PENALTY=0.472938
PRIMER_LEFT_15_PENALTY=0.428481
PRIMER_RIGHT_15_PENALTY=0.044457
PRIMER_LEFT_15_SEQUENCE=TGACGGTGCCAGTAAACATC
PRIMER_RIGHT_15_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_15=598,20
PRIMER_RIGHT_15=1528,20
PRIMER_LEFT_15_TM=59.572
PRIMER_RIGHT_15_TM=60.044
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.3000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=931
PRIMER_PAIR_16_PENALTY=0.473128
PRIMER_LEFT_16_PENALTY=0.041642
PRIMER_RIGHT_16_PENALTY=0.431486
PRIMER_LEFT_16_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_16_SEQUENCE=GTTCTGAGGTTTTCCCGTTG
PRIMER_LEFT_16=760,20
PRIMER_RIGHT_16=1454,20
PRIMER_LEFT_16_TM=60.042
PRIMER_RIGHT_16_TM=59.569
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=8.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=695
PRIMER_PAIR_17_PENALTY=0.542851
PRIMER_LEFT_17_PENALTY=0.111365
PRIMER_RIGHT_17_PENALTY=0.431486
PRIMER_LEFT_17_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_17_SEQUENCE=GTTCTGAGGTTTTCCCGTTG
PRIMER_LEFT_17=604,20
PRIMER_RIGHT_17=1454,20
PRIMER_LEFT_17_TM=60.111
PRIMER_RIGHT_17_TM=59.569
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=851
PRIMER_PAIR_18_PENALTY=0.615851
PRIMER_LEFT_18_PENALTY=0.010545
PRIMER_RIGHT_18_PENALTY=0.605305
PRIMER_LEFT_18_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_18_SEQUENCE=TTCTGAGGTTTTCCCGTTGA
PRIMER_LEFT_18=586,20
PRIMER_RIGHT_18=1453,20
PRIMER_LEFT_18_TM=59.989
PRIMER_RIGHT_18_TM=60.605
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=868
PRIMER_PAIR_19_PENALTY=0.646947
PRIMER_LEFT_19_PENALTY=0.041642
PRIMER_RIGHT_19_PENALTY=0.605305
PRIMER_LEFT_19_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_19_SEQUENCE=TTCTGAGGTTTTCCCGTTGA
PRIMER_LEFT_19=760,20
PRIMER_RIGHT_19=1453,20
PRIMER_LEFT_19_TM=60.042
PRIMER_RIGHT_19_TM=60.605
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=694
PRIMER_PAIR_20_PENALTY=0.651347
PRIMER_LEFT_20_PENALTY=0.010545
PRIMER_RIGHT_20_PENALTY=0.640802
PRIMER_LEFT_20_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_20_SEQUENCE=GTTTCGTGGCGTTTTTCTCT
PRIMER_LEFT_20=586,20
PRIMER_RIGHT_20=1505,20
PRIMER_LEFT_20_TM=59.989
PRIMER_RIGHT_20_TM=59.359
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.9000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=920
PRIMER_PAIR_21_PENALTY=0.651347
PRIMER_LEFT_21_PENALTY=0.010545
PRIMER_RIGHT_21_PENALTY=0.640802
PRIMER_LEFT_21_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_21_SEQUENCE=AGTTTCGTGGCGTTTTTCTC
PRIMER_LEFT_21=586,20
PRIMER_RIGHT_21=1506,20
PRIMER_LEFT_21_TM=59.989
PRIMER_RIGHT_21_TM=59.359
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=921
PRIMER_PAIR_22_PENALTY=0.679144
PRIMER_LEFT_22_PENALTY=0.384450
PRIMER_RIGHT_22_PENALTY=0.294694
PRIMER_LEFT_22_SEQUENCE=AAAGCAAAATCGCAGCAAAC
PRIMER_RIGHT_22_SEQUENCE=CTGAGGTTTTCCCGTTGAAG
PRIMER_LEFT_22=631,20
PRIMER_RIGHT_22=1451,20
PRIMER_LEFT_22_TM=60.384
PRIMER_RIGHT_22_TM=59.705
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=821
PRIMER_PAIR_23_PENALTY=0.682444
PRIMER_LEFT_23_PENALTY=0.041642
PRIMER_RIGHT_23_PENALTY=0.640802
PRIMER_LEFT_23_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_23_SEQUENCE=GTTTCGTGGCGTTTTTCTCT
PRIMER_LEFT_23=760,20
PRIMER_RIGHT_23=1505,20
PRIMER_LEFT_23_TM=60.042
PRIMER_RIGHT_23_TM=59.359
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=746
PRIMER_PAIR_24_PENALTY=0.682444
PRIMER_LEFT_24_PENALTY=0.041642
PRIMER_RIGHT_24_PENALTY=0.640802
PRIMER_LEFT_24_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_24_SEQUENCE=AGTTTCGTGGCGTTTTTCTC
PRIMER_LEFT_24=760,20
PRIMER_RIGHT_24=1506,20
PRIMER_LEFT_24_TM=60.042
PRIMER_RIGHT_24_TM=59.359
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=747
PRIMER_PAIR_25_PENALTY=0.707156
PRIMER_LEFT_25_PENALTY=0.384450
PRIMER_RIGHT_25_PENALTY=0.322707
PRIMER_LEFT_25_SEQUENCE=AAAGCAAAATCGCAGCAAAC
PRIMER_RIGHT_25_SEQUENCE=TTACAATGGCCCAAAATGCT
PRIMER_LEFT_25=631,20
PRIMER_RIGHT_25=1574,20
PRIMER_LEFT_25_TM=60.384
PRIMER_RIGHT_25_TM=60.323
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.1000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=944
PRIMER_PAIR_26_PENALTY=0.707156
PRIMER_LEFT_26_PENALTY=0.384450
PRIMER_RIGHT_26_PENALTY=0.322707
PRIMER_LEFT_26_SEQUENCE=AAAGCAAAATCGCAGCAAAC
PRIMER_RIGHT_26_SEQUENCE=GCTTACAATGGCCCAAAATG
PRIMER_LEFT_26=631,20
PRIMER_RIGHT_26=1576,20
PRIMER_LEFT_26_TM=60.384
PRIMER_RIGHT_26_TM=60.323
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=946
PRIMER_PAIR_27_PENALTY=0.716671
PRIMER_LEFT_27_PENALTY=0.111365
PRIMER_RIGHT_27_PENALTY=0.605305
PRIMER_LEFT_27_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_27_SEQUENCE=TTCTGAGGTTTTCCCGTTGA
PRIMER_LEFT_27=604,20
PRIMER_RIGHT_27=1453,20
PRIMER_LEFT_27_TM=60.111
PRIMER_RIGHT_27_TM=60.605
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=850
PRIMER_PAIR_28_PENALTY=0.723176
PRIMER_LEFT_28_PENALTY=0.428481
PRIMER_RIGHT_28_PENALTY=0.294694
PRIMER_LEFT_28_SEQUENCE=GTGCCAGTAAACATCGACCA
PRIMER_RIGHT_28_SEQUENCE=CTGAGGTTTTCCCGTTGAAG
PRIMER_LEFT_28=603,20
PRIMER_RIGHT_28=1451,20
PRIMER_LEFT_28_TM=59.572
PRIMER_RIGHT_28_TM=59.705
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=849
PRIMER_PAIR_29_PENALTY=0.723176
PRIMER_LEFT_29_PENALTY=0.428481
PRIMER_RIGHT_29_PENALTY=0.294694
PRIMER_LEFT_29_SEQUENCE=TGACGGTGCCAGTAAACATC
PRIMER_RIGHT_29_SEQUENCE=CTGAGGTTTTCCCGTTGAAG
PRIMER_LEFT_29=598,20
PRIMER_RIGHT_29=1451,20
PRIMER_LEFT_29_TM=59.572
PRIMER_RIGHT_29_TM=59.705
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.3000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=854
PRIMER_PAIR_30_PENALTY=0.751188
PRIMER_LEFT_30_PENALTY=0.428481
PRIMER_RIGHT_30_PENALTY=0.322707
PRIMER_LEFT_30_SEQUENCE=GTGCCAGTAAACATCGACCA
PRIMER_RIGHT_30_SEQUENCE=TTACAATGGCCCAAAATGCT
PRIMER_LEFT_30=603,20
PRIMER_RIGHT_30=1574,20
PRIMER_LEFT_30_TM=59.572
PRIMER_RIGHT_30_TM=60.323
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=972
PRIMER_PAIR_31_PENALTY=0.751188
PRIMER_LEFT_31_PENALTY=0.428481
PRIMER_RIGHT_31_PENALTY=0.322707
PRIMER_LEFT_31_SEQUENCE=GTGCCAGTAAACATCGACCA
PRIMER_RIGHT_31_SEQUENCE=GCTTACAATGGCCCAAAATG
PRIMER_LEFT_31=603,20
PRIMER_RIGHT_31=1576,20
PRIMER_LEFT_31_TM=59.572
PRIMER_RIGHT_31_TM=60.323
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=7.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=974
PRIMER_PAIR_32_PENALTY=0.751188
PRIMER_LEFT_32_PENALTY=0.428481
PRIMER_RIGHT_32_PENALTY=0.322707
PRIMER_LEFT_32_SEQUENCE=TGACGGTGCCAGTAAACATC
PRIMER_RIGHT_32_SEQUENCE=TTACAATGGCCCAAAATGCT
PRIMER_LEFT_32=598,20
PRIMER_RIGHT_32=1574,20
PRIMER_LEFT_32_TM=59.572
PRIMER_RIGHT_32_TM=60.323
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.3000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=977
PRIMER_PAIR_33_PENALTY=0.751188
PRIMER_LEFT_33_PENALTY=0.428481
PRIMER_RIGHT_33_PENALTY=0.322707
PRIMER_LEFT_33_SEQUENCE=TGACGGTGCCAGTAAACATC
PRIMER_RIGHT_33_SEQUENCE=GCTTACAATGGCCCAAAATG
PRIMER_LEFT_33=598,20
PRIMER_RIGHT_33=1576,20
PRIMER_LEFT_33_TM=59.572
PRIMER_RIGHT_33_TM=60.323
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.3000
PRIMER_RIGHT_33_END_STABILITY=7.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=979
PRIMER_PAIR_34_PENALTY=0.752167
PRIMER_LEFT_34_PENALTY=0.111365
PRIMER_RIGHT_34_PENALTY=0.640802
PRIMER_LEFT_34_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_34_SEQUENCE=GTTTCGTGGCGTTTTTCTCT
PRIMER_LEFT_34=604,20
PRIMER_RIGHT_34=1505,20
PRIMER_LEFT_34_TM=60.111
PRIMER_RIGHT_34_TM=59.359
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=902
PRIMER_PAIR_35_PENALTY=0.752167
PRIMER_LEFT_35_PENALTY=0.111365
PRIMER_RIGHT_35_PENALTY=0.640802
PRIMER_LEFT_35_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_35_SEQUENCE=AGTTTCGTGGCGTTTTTCTC
PRIMER_LEFT_35=604,20
PRIMER_RIGHT_35=1506,20
PRIMER_LEFT_35_TM=60.111
PRIMER_RIGHT_35_TM=59.359
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=903
PRIMER_PAIR_36_PENALTY=0.758758
PRIMER_LEFT_36_PENALTY=0.010545
PRIMER_RIGHT_36_PENALTY=0.748212
PRIMER_LEFT_36_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_36_SEQUENCE=GGCTTATAGTTTCGTGGCGT
PRIMER_LEFT_36=586,20
PRIMER_RIGHT_36=1513,20
PRIMER_LEFT_36_TM=59.989
PRIMER_RIGHT_36_TM=59.252
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.9000
PRIMER_RIGHT_36_END_STABILITY=11.1000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=928
PRIMER_PAIR_37_PENALTY=0.789854
PRIMER_LEFT_37_PENALTY=0.041642
PRIMER_RIGHT_37_PENALTY=0.748212
PRIMER_LEFT_37_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_37_SEQUENCE=GGCTTATAGTTTCGTGGCGT
PRIMER_LEFT_37=760,20
PRIMER_RIGHT_37=1513,20
PRIMER_LEFT_37_TM=60.042
PRIMER_RIGHT_37_TM=59.252
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=11.1000
PRIMER_PAIR_37_COMPL_ANY=2.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=754
PRIMER_PAIR_38_PENALTY=0.806785
PRIMER_LEFT_38_PENALTY=0.041642
PRIMER_RIGHT_38_PENALTY=0.765143
PRIMER_LEFT_38_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_38_SEQUENCE=GCGTTGACAATGTTGGGATT
PRIMER_LEFT_38=760,20
PRIMER_RIGHT_38=1599,20
PRIMER_LEFT_38_TM=60.042
PRIMER_RIGHT_38_TM=60.765
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=840
PRIMER_PAIR_39_PENALTY=0.813246
PRIMER_LEFT_39_PENALTY=0.768789
PRIMER_RIGHT_39_PENALTY=0.044457
PRIMER_LEFT_39_SEQUENCE=TGCATCGTCCTCCTTCATCT
PRIMER_RIGHT_39_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_39=761,20
PRIMER_RIGHT_39=1528,20
PRIMER_LEFT_39_TM=60.769
PRIMER_RIGHT_39_TM=60.044
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=768
PRIMER_PAIR_40_PENALTY=0.819908
PRIMER_LEFT_40_PENALTY=0.041642
PRIMER_RIGHT_40_PENALTY=0.778266
PRIMER_LEFT_40_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_40_SEQUENCE=TTGACAATGTTGGGATTTGG
PRIMER_LEFT_40=760,20
PRIMER_RIGHT_40=1596,20
PRIMER_LEFT_40_TM=60.042
PRIMER_RIGHT_40_TM=59.222
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.6000
PRIMER_RIGHT_40_END_STABILITY=8.8000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=837
PRIMER_PAIR_41_PENALTY=0.827481
PRIMER_LEFT_41_PENALTY=0.010545
PRIMER_RIGHT_41_PENALTY=0.816936
PRIMER_LEFT_41_SEQUENCE=ATAACCGGAGAGTGACGGTG
PRIMER_RIGHT_41_SEQUENCE=CAGTTCTGAGGTTTTCCCGT
PRIMER_LEFT_41=586,20
PRIMER_RIGHT_41=1456,20
PRIMER_LEFT_41_TM=59.989
PRIMER_RIGHT_41_TM=59.183
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=11.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=871
PRIMER_PAIR_42_PENALTY=0.831557
PRIMER_LEFT_42_PENALTY=0.787100
PRIMER_RIGHT_42_PENALTY=0.044457
PRIMER_LEFT_42_SEQUENCE=AGTGACGGTGCCAGTAAACA
PRIMER_RIGHT_42_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_42=596,20
PRIMER_RIGHT_42=1528,20
PRIMER_LEFT_42_TM=59.213
PRIMER_RIGHT_42_TM=60.044
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=9.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=933
PRIMER_PAIR_43_PENALTY=0.848503
PRIMER_LEFT_43_PENALTY=0.768789
PRIMER_RIGHT_43_PENALTY=0.079714
PRIMER_LEFT_43_SEQUENCE=TGCATCGTCCTCCTTCATCT
PRIMER_RIGHT_43_SEQUENCE=AAGCTTTTGTTGACCAGCGT
PRIMER_LEFT_43=761,20
PRIMER_RIGHT_43=1635,20
PRIMER_LEFT_43_TM=60.769
PRIMER_RIGHT_43_TM=59.920
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=875
PRIMER_PAIR_44_PENALTY=0.858578
PRIMER_LEFT_44_PENALTY=0.041642
PRIMER_RIGHT_44_PENALTY=0.816936
PRIMER_LEFT_44_SEQUENCE=ATGCATCGTCCTCCTTCATC
PRIMER_RIGHT_44_SEQUENCE=CAGTTCTGAGGTTTTCCCGT
PRIMER_LEFT_44=760,20
PRIMER_RIGHT_44=1456,20
PRIMER_LEFT_44_TM=60.042
PRIMER_RIGHT_44_TM=59.183
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.6000
PRIMER_RIGHT_44_END_STABILITY=11.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=697
PRIMER_PAIR_45_PENALTY=0.859578
PRIMER_LEFT_45_PENALTY=0.111365
PRIMER_RIGHT_45_PENALTY=0.748212
PRIMER_LEFT_45_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_45_SEQUENCE=GGCTTATAGTTTCGTGGCGT
PRIMER_LEFT_45=604,20
PRIMER_RIGHT_45=1513,20
PRIMER_LEFT_45_TM=60.111
PRIMER_RIGHT_45_TM=59.252
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=11.1000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=910
PRIMER_PAIR_46_PENALTY=0.859967
PRIMER_LEFT_46_PENALTY=0.428481
PRIMER_RIGHT_46_PENALTY=0.431486
PRIMER_LEFT_46_SEQUENCE=GTGCCAGTAAACATCGACCA
PRIMER_RIGHT_46_SEQUENCE=GTTCTGAGGTTTTCCCGTTG
PRIMER_LEFT_46=603,20
PRIMER_RIGHT_46=1454,20
PRIMER_LEFT_46_TM=59.572
PRIMER_RIGHT_46_TM=59.569
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=8.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=852
PRIMER_PAIR_47_PENALTY=0.859967
PRIMER_LEFT_47_PENALTY=0.428481
PRIMER_RIGHT_47_PENALTY=0.431486
PRIMER_LEFT_47_SEQUENCE=TGACGGTGCCAGTAAACATC
PRIMER_RIGHT_47_SEQUENCE=GTTCTGAGGTTTTCCCGTTG
PRIMER_LEFT_47=598,20
PRIMER_RIGHT_47=1454,20
PRIMER_LEFT_47_TM=59.572
PRIMER_RIGHT_47_TM=59.569
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.3000
PRIMER_RIGHT_47_END_STABILITY=8.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=857
PRIMER_PAIR_48_PENALTY=0.870697
PRIMER_LEFT_48_PENALTY=0.826240
PRIMER_RIGHT_48_PENALTY=0.044457
PRIMER_LEFT_48_SEQUENCE=CCCGGTAGATAACCGGAGAG
PRIMER_RIGHT_48_SEQUENCE=ACTGAGCATAGTTGCGGCTT
PRIMER_LEFT_48=578,20
PRIMER_RIGHT_48=1528,20
PRIMER_LEFT_48_TM=60.826
PRIMER_RIGHT_48_TM=60.044
PRIMER_LEFT_48_GC_PERCENT=60.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=951
PRIMER_PAIR_49_PENALTY=0.876508
PRIMER_LEFT_49_PENALTY=0.111365
PRIMER_RIGHT_49_PENALTY=0.765143
PRIMER_LEFT_49_SEQUENCE=TGCCAGTAAACATCGACCAA
PRIMER_RIGHT_49_SEQUENCE=GCGTTGACAATGTTGGGATT
PRIMER_LEFT_49=604,20
PRIMER_RIGHT_49=1599,20
PRIMER_LEFT_49_TM=60.111
PRIMER_RIGHT_49_TM=60.765
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=8.1000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=996
=
SEQUENCE_ID=MAGIC.149_4_4251919_4251928_edi-0_ws-0_19081_64784_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4725120_4725331_rsch-4_edi-0_4398_2905_TRUE
SEQUENCE_TARGET=950,311
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_1317091_1317096_tsu-0_rsch-4_25063_142912_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTANTGACAAANCAGAGCAAAAGACAGTAAAGTTTCTTGCTTTATTCAAAAACTAAAACCAAAGAAAAATCACATTACTAATTTGCTTATAAACACTCTGCGACTAATAACCGTTGGATCATATCAGTAAGGAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATCACGAAATTAGCAAACTCTCGTCACAAATCATCAGTTTCTCTTTCTCTCTTCCTCNTTAGTCTCTCTCCACTGATCTTGAAACTACAACNATGAACGTGTACATCGATACTGAGACTGGCTCATCGTTCTCCATTACTATTGATTTCGGCGAAACAGTGTTANAGATNAAAGAGAAGATCGAGAAGTCTCAAGGTATCCCTGTTTCGAAACAAATCCTTTACTTGGATGNCAAGGCTCTTGAANNNGATCTTCATAAGATTGATTACATGATCCTCTTCGAATCTCGTCTCCTTCTCCGCATCTCTCCTGATGCTNACCCTAATCAGAGCAACGAGCAAACCGAGCAATCAAAACAAATTGATGATAAGAAACAAGAGTTCTGTGGCATTCAAGATTCATCGGAGAGCAAAAAGNTCACGAGGGTGATGGCGAGGAGAGTCCACAATATATATTCATCACTACCGGCTTATTCACTTGATGAGTTACTTGGCCCTAAATATTCAGCAACTGTGNCGGTTGGAGGCAGAACAAATCAAGTGGTTCAANCCACCGAGCAAGCTTCAACGTCAGGCACAGCCAAAGAGGTCCTTAGAGATTCAGATTCGCCTGTGGANAAGAAGATCAAGACCAACCCGATGAAGTTTACAGTACATGTGAAGCCATATCAAGAAGATACCANGATGATT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_9954027_9954185_edi-0_col-0_3208804_3811_TRUE
SEQUENCE_TARGET=950,258
SEQUENCE_TEMPLATE=GCATGTAATAAGCATCTACAAGGAAAAGATCATTCAGAGTAGATGGAAGATAAAAGCAGAGTTGGCTTAAACTAAAACTAAGCAGTGTCATATACCAGAGCAACTCTATAAGAAGATACACTTGCTCTAAACGAGAGAGACTGTCCTCTCTGCAACCCTGAAAAAGATCTAGTTATAAAACCTTAAGTTAGCTAAAGTTAAACCCTTAACTAACANATGACTTACAAGTTATACCTTTTGGCAGTCACAGAAAGAATCAGTACCAACAACGACAACAAGNCTGTCCCATGATAAACCCATTCTGATAGAACCTGTAATATATAAGTTTTGGTGATCATCAGAGAACATTAAAAGTAGGCACTGGTGANTCAAAAAGTTTGAAGAGAGCGTACAGTGAAAAAGAGGAGCATGGGGAAGACAATGAAGATGAAATCCAATGATATGGCAGCCTTCCAATTTCTGGAAATAACAATTTTCTCATCGTCGTTTNTCTTCGATGTAACGGNTTTAACACCATTGTTATCTATAACAAACAAGTNAACATCTTTAGCACTAATCGAACAAAAGTTACATCACTCACTTCAAAAAGCACTCACCAATTTTACAGTGGAAGCCAATGGAGTATCGTAATAGAACCAAGAGCTGAAACAAGAGAAACGTCATTATTATCAGGAATTGAGCTAAACTGAGTCAAAAGAAACTTACAGGGACAATGGTCAATAGTGCTGCGATTTCCAGAATTGAAGATCCATCGAGATTGCTCACAAATCTGTCAAAAGTCAATTTGAACAAAAAAANCTTGAGGAATCACAAATTCTAAAANNCTGACGAANGAGAGNTCATTGAGNGAGTAAACAAAAAGAGAGNNGNNTTTTACTGTTCTTTGAGATGTTTGTTTGGATTTAGAGTCTGCTTTGGTANAGAATCCATCAANACTCNGAGATCAAANAAAGTTGTNAACTTNGAACAGTCGTNCTCGTTGATNAACTATANANATTTANTTTGGTTTGGATCACGAACCGGCCGGTTTANNCNNNATAATCTCAGNTTNCTTTTGTTATTGTTACGAATCTATGCTCGATTTCACATTAAACCAAACATGGACTCACTAATCTGCNTCANACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_14273400_14273788_rsch-4_ct-1_122660_22031_TRUE
SEQUENCE_TARGET=950,488
SEQUENCE_TEMPLATE=ACTTGATAGTGATTTTTGGATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_725254_725295_sf-2_ws-0_392380_68683_TRUE
SEQUENCE_TARGET=950,141
SEQUENCE_TEMPLATE=CTNCCTTAACCTGCAATATTTGTGGTTTGGTAAATAAACTTNATCTNACCTACATTTNCCCTATATGCGATTTNGTNGCCCATAGTGATTGTATNTATATCCCNCAAANCATAAGAANATCTCGTCACCACCATCGTGTNTCTTTTATTTCTTCCCTTNCNTTTGAAGAAAAGTNTTGCGGAGTTTGNCGTCAAATGCTTGACAGTGATTATGGTGCATATATTTGCAAAGGGTGTAGTGGCTTTGTCGNTCACNTAAGATGTGCANTACGTANTGATATATGGGATGGNNAAGAACTNGAAGGAGTACCAGNAGAAGTAGAAGAAGATGACGTTGAATCCTTCAAGAGGATAGCTGATGGAGTAATACTCCATTTTTCTCATNNCCATCATCTGNATTTTGAGATAAGNGGAGTTTACAATGGAAACAAGTTTTGTCAAGCTTGTGNCCTTCCANTCAANGAAGGNAATTTNTATGTATGTGTNGNATGTGATTTCATCCTACACGAAACATGTGCAGATGCTCCACGTAGAAAGGTCCATCCATTACATCCCCATCCCCTTAAANNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNGCGTANGAGTACTGNTTTTNGATATNGGTGTANNAACCANAATTGCAAGTACATGATAGATATAGTNTGTGCCTCGACTGCTGAGCCGTTTGATTATCAAGGCCATCGACATCCCTTATTCTTATCGTTAGACCCAAAAGAGAAGCCAATGTGCCACATTTGCANGTCAACAAAAGATAAGAAGGTACTTAACTGCATTGAATGTGACTTTATTNTATGTTTCGAATGCGCCACCTTACCATACATGATAAGGTATAAGCATGATGAACATTACCTCACATTTTGTCATGGNGATGAGGCAAGNGANTCAGNTTGGTGTGAATTATGTGAGGGNNAGTTAGCAATTGGAGGAANAGAAGGNTTCTATNAGTGCANNNATTGTTGNACNACNCTTCATATTAATTGNTTNCTTGGNCCAGATCNATANCNTANNGNTGGTCAAAGNNNCNNANTTNNTAACGGTGNGATTNTTNTTCGACNCAACAANTNTACTACTCGGCCTTTTTGCTCGATNTGCAANATNCGTTGTCCGTATCNAANANTTNTNNNATACATANNGNATATGTNTNNATCTTATANTCTATGTTCTAGTAANCACTTNAANANGAGNTACNATCATTGAGTTATCCCTTTTCTTTTAAAATTTTNGTTGTNNTTTTACTTTNATTTTNNATGTACACCTGNAACCCTTTNTGTAATATATTGTATTTTTGGTTNGATCTTTTTGTTAACNCTGTTTNTGTGNGTTTGGTTTAANAATATATACTTNAAANTTTAACTTATGCTTTAAAACTTCATCTGATTNTNTNAGACTAAATCATTGAATTTTNTTAAAATACNGTGAAATTNNTCTATAGGTNAAAAATATNNAACTNGAAAGTAAAAGNTCGANATTATTANATCTAGGTGTACCTCNNANNCTTTTTNTAATATGTATTGTTGGTTCTTTTTGCCAACCTCGTTTGTCTGTGTTTGATGTAATATCTATTATACATNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.237040
PRIMER_LEFT_0_PENALTY=0.148627
PRIMER_RIGHT_0_PENALTY=0.088413
PRIMER_LEFT_0_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_0_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_0=834,20
PRIMER_RIGHT_0=1569,20
PRIMER_LEFT_0_TM=59.851
PRIMER_RIGHT_0_TM=60.088
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=736
PRIMER_PAIR_1_PENALTY=0.424376
PRIMER_LEFT_1_PENALTY=0.148627
PRIMER_RIGHT_1_PENALTY=0.275749
PRIMER_LEFT_1_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_1_SEQUENCE=AACGAGGTTGGCAAAAAGAA
PRIMER_LEFT_1=834,20
PRIMER_RIGHT_1=1572,20
PRIMER_LEFT_1_TM=59.851
PRIMER_RIGHT_1_TM=59.724
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=739
PRIMER_PAIR_2_PENALTY=0.424376
PRIMER_LEFT_2_PENALTY=0.148627
PRIMER_RIGHT_2_PENALTY=0.275749
PRIMER_LEFT_2_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_2_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_2=834,20
PRIMER_RIGHT_2=1573,20
PRIMER_LEFT_2_TM=59.851
PRIMER_RIGHT_2_TM=59.724
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=740
PRIMER_PAIR_3_PENALTY=0.562089
PRIMER_LEFT_3_PENALTY=0.148627
PRIMER_RIGHT_3_PENALTY=0.413463
PRIMER_LEFT_3_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_3_SEQUENCE=AGGTTGGCAAAAAGAACCAA
PRIMER_LEFT_3=834,20
PRIMER_RIGHT_3=1568,20
PRIMER_LEFT_3_TM=59.851
PRIMER_RIGHT_3_TM=59.587
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=735
PRIMER_PAIR_4_PENALTY=0.662344
PRIMER_LEFT_4_PENALTY=0.148627
PRIMER_RIGHT_4_PENALTY=0.513718
PRIMER_LEFT_4_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_4_SEQUENCE=ACAAACGAGGTTGGCAAAAA
PRIMER_LEFT_4=834,20
PRIMER_RIGHT_4=1575,20
PRIMER_LEFT_4_TM=59.851
PRIMER_RIGHT_4_TM=60.514
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=742
PRIMER_PAIR_5_PENALTY=0.696135
PRIMER_LEFT_5_PENALTY=0.148627
PRIMER_RIGHT_5_PENALTY=0.547508
PRIMER_LEFT_5_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_5_SEQUENCE=GGTTGGCAAAAAGAACCAAC
PRIMER_LEFT_5=834,20
PRIMER_RIGHT_5=1567,20
PRIMER_LEFT_5_TM=59.851
PRIMER_RIGHT_5_TM=59.452
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=5.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=734
PRIMER_PAIR_6_PENALTY=0.740300
PRIMER_LEFT_6_PENALTY=0.651888
PRIMER_RIGHT_6_PENALTY=0.088413
PRIMER_LEFT_6_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_6_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_6=711,20
PRIMER_RIGHT_6=1569,20
PRIMER_LEFT_6_TM=59.348
PRIMER_RIGHT_6_TM=60.088
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=859
PRIMER_PAIR_7_PENALTY=0.787798
PRIMER_LEFT_7_PENALTY=0.148627
PRIMER_RIGHT_7_PENALTY=0.639172
PRIMER_LEFT_7_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_7_SEQUENCE=CAAACGAGGTTGGCAAAAAG
PRIMER_LEFT_7=834,20
PRIMER_RIGHT_7=1574,20
PRIMER_LEFT_7_TM=59.851
PRIMER_RIGHT_7_TM=60.639
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=741
PRIMER_PAIR_8_PENALTY=0.917974
PRIMER_LEFT_8_PENALTY=0.829561
PRIMER_RIGHT_8_PENALTY=0.088413
PRIMER_LEFT_8_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_8_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_8=742,20
PRIMER_RIGHT_8=1569,20
PRIMER_LEFT_8_TM=59.170
PRIMER_RIGHT_8_TM=60.088
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=828
PRIMER_PAIR_9_PENALTY=0.927637
PRIMER_LEFT_9_PENALTY=0.651888
PRIMER_RIGHT_9_PENALTY=0.275749
PRIMER_LEFT_9_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_9_SEQUENCE=AACGAGGTTGGCAAAAAGAA
PRIMER_LEFT_9=711,20
PRIMER_RIGHT_9=1572,20
PRIMER_LEFT_9_TM=59.348
PRIMER_RIGHT_9_TM=59.724
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.9000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=862
PRIMER_PAIR_10_PENALTY=0.927637
PRIMER_LEFT_10_PENALTY=0.651888
PRIMER_RIGHT_10_PENALTY=0.275749
PRIMER_LEFT_10_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_10_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_10=711,20
PRIMER_RIGHT_10=1573,20
PRIMER_LEFT_10_TM=59.348
PRIMER_RIGHT_10_TM=59.724
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=863
PRIMER_PAIR_11_PENALTY=0.930135
PRIMER_LEFT_11_PENALTY=0.148627
PRIMER_RIGHT_11_PENALTY=0.781508
PRIMER_LEFT_11_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_11_SEQUENCE=ACGAGGTTGGCAAAAAGAAC
PRIMER_LEFT_11=834,20
PRIMER_RIGHT_11=1571,20
PRIMER_LEFT_11_TM=59.851
PRIMER_RIGHT_11_TM=59.218
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.6000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=738
PRIMER_PAIR_12_PENALTY=1.065350
PRIMER_LEFT_12_PENALTY=0.651888
PRIMER_RIGHT_12_PENALTY=0.413463
PRIMER_LEFT_12_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_12_SEQUENCE=AGGTTGGCAAAAAGAACCAA
PRIMER_LEFT_12=711,20
PRIMER_RIGHT_12=1568,20
PRIMER_LEFT_12_TM=59.348
PRIMER_RIGHT_12_TM=59.587
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=858
PRIMER_PAIR_13_PENALTY=1.098020
PRIMER_LEFT_13_PENALTY=1.009608
PRIMER_RIGHT_13_PENALTY=0.088413
PRIMER_LEFT_13_SEQUENCE=ACCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_13_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_13=741,21
PRIMER_RIGHT_13=1569,20
PRIMER_LEFT_13_TM=59.990
PRIMER_RIGHT_13_TM=60.088
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=829
PRIMER_PAIR_14_PENALTY=1.105310
PRIMER_LEFT_14_PENALTY=0.829561
PRIMER_RIGHT_14_PENALTY=0.275749
PRIMER_LEFT_14_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_14_SEQUENCE=AACGAGGTTGGCAAAAAGAA
PRIMER_LEFT_14=742,20
PRIMER_RIGHT_14=1572,20
PRIMER_LEFT_14_TM=59.170
PRIMER_RIGHT_14_TM=59.724
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=831
PRIMER_PAIR_15_PENALTY=1.105310
PRIMER_LEFT_15_PENALTY=0.829561
PRIMER_RIGHT_15_PENALTY=0.275749
PRIMER_LEFT_15_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_15_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_15=742,20
PRIMER_RIGHT_15=1573,20
PRIMER_LEFT_15_TM=59.170
PRIMER_RIGHT_15_TM=59.724
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=832
PRIMER_PAIR_16_PENALTY=1.132723
PRIMER_LEFT_16_PENALTY=1.044310
PRIMER_RIGHT_16_PENALTY=0.088413
PRIMER_LEFT_16_SEQUENCE=GGCCATCGACATCCCTTATT
PRIMER_RIGHT_16_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_16=710,20
PRIMER_RIGHT_16=1569,20
PRIMER_LEFT_16_TM=61.044
PRIMER_RIGHT_16_TM=60.088
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.2000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=860
PRIMER_PAIR_17_PENALTY=1.165605
PRIMER_LEFT_17_PENALTY=0.651888
PRIMER_RIGHT_17_PENALTY=0.513718
PRIMER_LEFT_17_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_17_SEQUENCE=ACAAACGAGGTTGGCAAAAA
PRIMER_LEFT_17=711,20
PRIMER_RIGHT_17=1575,20
PRIMER_LEFT_17_TM=59.348
PRIMER_RIGHT_17_TM=60.514
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.9000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=865
PRIMER_PAIR_18_PENALTY=1.179652
PRIMER_LEFT_18_PENALTY=1.091240
PRIMER_RIGHT_18_PENALTY=0.088413
PRIMER_LEFT_18_SEQUENCE=AGACCCAAAAGAGAAGCCAA
PRIMER_RIGHT_18_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_18=739,20
PRIMER_RIGHT_18=1569,20
PRIMER_LEFT_18_TM=58.909
PRIMER_RIGHT_18_TM=60.088
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=10.0000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=831
PRIMER_PAIR_19_PENALTY=1.199395
PRIMER_LEFT_19_PENALTY=0.651888
PRIMER_RIGHT_19_PENALTY=0.547508
PRIMER_LEFT_19_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_19_SEQUENCE=GGTTGGCAAAAAGAACCAAC
PRIMER_LEFT_19=711,20
PRIMER_RIGHT_19=1567,20
PRIMER_LEFT_19_TM=59.348
PRIMER_RIGHT_19_TM=59.452
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=857
PRIMER_PAIR_20_PENALTY=1.243024
PRIMER_LEFT_20_PENALTY=0.829561
PRIMER_RIGHT_20_PENALTY=0.413463
PRIMER_LEFT_20_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_20_SEQUENCE=AGGTTGGCAAAAAGAACCAA
PRIMER_LEFT_20=742,20
PRIMER_RIGHT_20=1568,20
PRIMER_LEFT_20_TM=59.170
PRIMER_RIGHT_20_TM=59.587
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=827
PRIMER_PAIR_21_PENALTY=1.285357
PRIMER_LEFT_21_PENALTY=1.009608
PRIMER_RIGHT_21_PENALTY=0.275749
PRIMER_LEFT_21_SEQUENCE=ACCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_21_SEQUENCE=AACGAGGTTGGCAAAAAGAA
PRIMER_LEFT_21=741,21
PRIMER_RIGHT_21=1572,20
PRIMER_LEFT_21_TM=59.990
PRIMER_RIGHT_21_TM=59.724
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=832
PRIMER_PAIR_22_PENALTY=1.285357
PRIMER_LEFT_22_PENALTY=1.009608
PRIMER_RIGHT_22_PENALTY=0.275749
PRIMER_LEFT_22_SEQUENCE=ACCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_22_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_22=741,21
PRIMER_RIGHT_22=1573,20
PRIMER_LEFT_22_TM=59.990
PRIMER_RIGHT_22_TM=59.724
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=833
PRIMER_PAIR_23_PENALTY=1.291059
PRIMER_LEFT_23_PENALTY=0.651888
PRIMER_RIGHT_23_PENALTY=0.639172
PRIMER_LEFT_23_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_23_SEQUENCE=CAAACGAGGTTGGCAAAAAG
PRIMER_LEFT_23=711,20
PRIMER_RIGHT_23=1574,20
PRIMER_LEFT_23_TM=59.348
PRIMER_RIGHT_23_TM=60.639
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=5.9000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=864
PRIMER_PAIR_24_PENALTY=1.320059
PRIMER_LEFT_24_PENALTY=1.044310
PRIMER_RIGHT_24_PENALTY=0.275749
PRIMER_LEFT_24_SEQUENCE=GGCCATCGACATCCCTTATT
PRIMER_RIGHT_24_SEQUENCE=AACGAGGTTGGCAAAAAGAA
PRIMER_LEFT_24=710,20
PRIMER_RIGHT_24=1572,20
PRIMER_LEFT_24_TM=61.044
PRIMER_RIGHT_24_TM=59.724
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.2000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=863
PRIMER_PAIR_25_PENALTY=1.320059
PRIMER_LEFT_25_PENALTY=1.044310
PRIMER_RIGHT_25_PENALTY=0.275749
PRIMER_LEFT_25_SEQUENCE=GGCCATCGACATCCCTTATT
PRIMER_RIGHT_25_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_25=710,20
PRIMER_RIGHT_25=1573,20
PRIMER_LEFT_25_TM=61.044
PRIMER_RIGHT_25_TM=59.724
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.2000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=864
PRIMER_PAIR_26_PENALTY=1.343279
PRIMER_LEFT_26_PENALTY=0.829561
PRIMER_RIGHT_26_PENALTY=0.513718
PRIMER_LEFT_26_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_26_SEQUENCE=ACAAACGAGGTTGGCAAAAA
PRIMER_LEFT_26=742,20
PRIMER_RIGHT_26=1575,20
PRIMER_LEFT_26_TM=59.170
PRIMER_RIGHT_26_TM=60.514
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=834
PRIMER_PAIR_27_PENALTY=1.366989
PRIMER_LEFT_27_PENALTY=1.091240
PRIMER_RIGHT_27_PENALTY=0.275749
PRIMER_LEFT_27_SEQUENCE=AGACCCAAAAGAGAAGCCAA
PRIMER_RIGHT_27_SEQUENCE=AACGAGGTTGGCAAAAAGAA
PRIMER_LEFT_27=739,20
PRIMER_RIGHT_27=1572,20
PRIMER_LEFT_27_TM=58.909
PRIMER_RIGHT_27_TM=59.724
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=10.0000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=834
PRIMER_PAIR_28_PENALTY=1.366989
PRIMER_LEFT_28_PENALTY=1.091240
PRIMER_RIGHT_28_PENALTY=0.275749
PRIMER_LEFT_28_SEQUENCE=AGACCCAAAAGAGAAGCCAA
PRIMER_RIGHT_28_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_28=739,20
PRIMER_RIGHT_28=1573,20
PRIMER_LEFT_28_TM=58.909
PRIMER_RIGHT_28_TM=59.724
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=10.0000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=835
PRIMER_PAIR_29_PENALTY=1.377069
PRIMER_LEFT_29_PENALTY=0.829561
PRIMER_RIGHT_29_PENALTY=0.547508
PRIMER_LEFT_29_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_29_SEQUENCE=GGTTGGCAAAAAGAACCAAC
PRIMER_LEFT_29=742,20
PRIMER_RIGHT_29=1567,20
PRIMER_LEFT_29_TM=59.170
PRIMER_RIGHT_29_TM=59.452
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=826
PRIMER_PAIR_30_PENALTY=1.388911
PRIMER_LEFT_30_PENALTY=1.300498
PRIMER_RIGHT_30_PENALTY=0.088413
PRIMER_LEFT_30_SEQUENCE=GCCATCGACATCCCTTATTCT
PRIMER_RIGHT_30_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_30=711,21
PRIMER_RIGHT_30=1569,20
PRIMER_LEFT_30_TM=60.300
PRIMER_RIGHT_30_TM=60.088
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=859
PRIMER_PAIR_31_PENALTY=1.392408
PRIMER_LEFT_31_PENALTY=1.303996
PRIMER_RIGHT_31_PENALTY=0.088413
PRIMER_LEFT_31_SEQUENCE=ATGCGCCACCTTACCATACA
PRIMER_RIGHT_31_SEQUENCE=GAGGTTGGCAAAAAGAACCA
PRIMER_LEFT_31=835,20
PRIMER_RIGHT_31=1569,20
PRIMER_LEFT_31_TM=61.304
PRIMER_RIGHT_31_TM=60.088
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.6000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=735
PRIMER_PAIR_32_PENALTY=1.423070
PRIMER_LEFT_32_PENALTY=1.009608
PRIMER_RIGHT_32_PENALTY=0.413463
PRIMER_LEFT_32_SEQUENCE=ACCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_32_SEQUENCE=AGGTTGGCAAAAAGAACCAA
PRIMER_LEFT_32=741,21
PRIMER_RIGHT_32=1568,20
PRIMER_LEFT_32_TM=59.990
PRIMER_RIGHT_32_TM=59.587
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=828
PRIMER_PAIR_33_PENALTY=1.433396
PRIMER_LEFT_33_PENALTY=0.651888
PRIMER_RIGHT_33_PENALTY=0.781508
PRIMER_LEFT_33_SEQUENCE=GCCATCGACATCCCTTATTC
PRIMER_RIGHT_33_SEQUENCE=ACGAGGTTGGCAAAAAGAAC
PRIMER_LEFT_33=711,20
PRIMER_RIGHT_33=1571,20
PRIMER_LEFT_33_TM=59.348
PRIMER_RIGHT_33_TM=59.218
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=5.9000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=861
PRIMER_PAIR_34_PENALTY=1.457773
PRIMER_LEFT_34_PENALTY=1.044310
PRIMER_RIGHT_34_PENALTY=0.413463
PRIMER_LEFT_34_SEQUENCE=GGCCATCGACATCCCTTATT
PRIMER_RIGHT_34_SEQUENCE=AGGTTGGCAAAAAGAACCAA
PRIMER_LEFT_34=710,20
PRIMER_RIGHT_34=1568,20
PRIMER_LEFT_34_TM=61.044
PRIMER_RIGHT_34_TM=59.587
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.2000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=859
PRIMER_PAIR_35_PENALTY=1.468733
PRIMER_LEFT_35_PENALTY=0.829561
PRIMER_RIGHT_35_PENALTY=0.639172
PRIMER_LEFT_35_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_35_SEQUENCE=CAAACGAGGTTGGCAAAAAG
PRIMER_LEFT_35=742,20
PRIMER_RIGHT_35=1574,20
PRIMER_LEFT_35_TM=59.170
PRIMER_RIGHT_35_TM=60.639
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=7.3000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=833
PRIMER_PAIR_36_PENALTY=1.499229
PRIMER_LEFT_36_PENALTY=0.148627
PRIMER_RIGHT_36_PENALTY=1.350602
PRIMER_LEFT_36_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_36_SEQUENCE=GTTGGCAAAAAGAACCAACA
PRIMER_LEFT_36=834,20
PRIMER_RIGHT_36=1566,20
PRIMER_LEFT_36_TM=59.851
PRIMER_RIGHT_36_TM=58.649
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.6000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=733
PRIMER_PAIR_37_PENALTY=1.504702
PRIMER_LEFT_37_PENALTY=1.091240
PRIMER_RIGHT_37_PENALTY=0.413463
PRIMER_LEFT_37_SEQUENCE=AGACCCAAAAGAGAAGCCAA
PRIMER_RIGHT_37_SEQUENCE=AGGTTGGCAAAAAGAACCAA
PRIMER_LEFT_37=739,20
PRIMER_RIGHT_37=1568,20
PRIMER_LEFT_37_TM=58.909
PRIMER_RIGHT_37_TM=59.587
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=10.0000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=830
PRIMER_PAIR_38_PENALTY=1.523325
PRIMER_LEFT_38_PENALTY=1.009608
PRIMER_RIGHT_38_PENALTY=0.513718
PRIMER_LEFT_38_SEQUENCE=ACCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_38_SEQUENCE=ACAAACGAGGTTGGCAAAAA
PRIMER_LEFT_38=741,21
PRIMER_RIGHT_38=1575,20
PRIMER_LEFT_38_TM=59.990
PRIMER_RIGHT_38_TM=60.514
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=835
PRIMER_PAIR_39_PENALTY=1.527247
PRIMER_LEFT_39_PENALTY=0.148627
PRIMER_RIGHT_39_PENALTY=1.378620
PRIMER_LEFT_39_SEQUENCE=AATGCGCCACCTTACCATAC
PRIMER_RIGHT_39_SEQUENCE=AGGTTGGCAAAAAGAACCAAC
PRIMER_LEFT_39=834,20
PRIMER_RIGHT_39=1568,21
PRIMER_LEFT_39_TM=59.851
PRIMER_RIGHT_39_TM=60.379
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.6000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=735
PRIMER_PAIR_40_PENALTY=1.557115
PRIMER_LEFT_40_PENALTY=1.009608
PRIMER_RIGHT_40_PENALTY=0.547508
PRIMER_LEFT_40_SEQUENCE=ACCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_40_SEQUENCE=GGTTGGCAAAAAGAACCAAC
PRIMER_LEFT_40=741,21
PRIMER_RIGHT_40=1567,20
PRIMER_LEFT_40_TM=59.990
PRIMER_RIGHT_40_TM=59.452
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.6000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=827
PRIMER_PAIR_41_PENALTY=1.558028
PRIMER_LEFT_41_PENALTY=1.044310
PRIMER_RIGHT_41_PENALTY=0.513718
PRIMER_LEFT_41_SEQUENCE=GGCCATCGACATCCCTTATT
PRIMER_RIGHT_41_SEQUENCE=ACAAACGAGGTTGGCAAAAA
PRIMER_LEFT_41=710,20
PRIMER_RIGHT_41=1575,20
PRIMER_LEFT_41_TM=61.044
PRIMER_RIGHT_41_TM=60.514
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.2000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=866
PRIMER_PAIR_42_PENALTY=1.576247
PRIMER_LEFT_42_PENALTY=1.300498
PRIMER_RIGHT_42_PENALTY=0.275749
PRIMER_LEFT_42_SEQUENCE=GCCATCGACATCCCTTATTCT
PRIMER_RIGHT_42_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_42=711,21
PRIMER_RIGHT_42=1573,20
PRIMER_LEFT_42_TM=60.300
PRIMER_RIGHT_42_TM=59.724
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=863
PRIMER_PAIR_43_PENALTY=1.579745
PRIMER_LEFT_43_PENALTY=1.303996
PRIMER_RIGHT_43_PENALTY=0.275749
PRIMER_LEFT_43_SEQUENCE=ATGCGCCACCTTACCATACA
PRIMER_RIGHT_43_SEQUENCE=AACGAGGTTGGCAAAAAGAA
PRIMER_LEFT_43=835,20
PRIMER_RIGHT_43=1572,20
PRIMER_LEFT_43_TM=61.304
PRIMER_RIGHT_43_TM=59.724
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=5.6000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=738
PRIMER_PAIR_44_PENALTY=1.579745
PRIMER_LEFT_44_PENALTY=1.303996
PRIMER_RIGHT_44_PENALTY=0.275749
PRIMER_LEFT_44_SEQUENCE=ATGCGCCACCTTACCATACA
PRIMER_RIGHT_44_SEQUENCE=AAACGAGGTTGGCAAAAAGA
PRIMER_LEFT_44=835,20
PRIMER_RIGHT_44=1573,20
PRIMER_LEFT_44_TM=61.304
PRIMER_RIGHT_44_TM=59.724
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=5.6000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=739
PRIMER_PAIR_45_PENALTY=1.591818
PRIMER_LEFT_45_PENALTY=1.044310
PRIMER_RIGHT_45_PENALTY=0.547508
PRIMER_LEFT_45_SEQUENCE=GGCCATCGACATCCCTTATT
PRIMER_RIGHT_45_SEQUENCE=GGTTGGCAAAAAGAACCAAC
PRIMER_LEFT_45=710,20
PRIMER_RIGHT_45=1567,20
PRIMER_LEFT_45_TM=61.044
PRIMER_RIGHT_45_TM=59.452
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.2000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=858
PRIMER_PAIR_46_PENALTY=1.604957
PRIMER_LEFT_46_PENALTY=1.091240
PRIMER_RIGHT_46_PENALTY=0.513718
PRIMER_LEFT_46_SEQUENCE=AGACCCAAAAGAGAAGCCAA
PRIMER_RIGHT_46_SEQUENCE=ACAAACGAGGTTGGCAAAAA
PRIMER_LEFT_46=739,20
PRIMER_RIGHT_46=1575,20
PRIMER_LEFT_46_TM=58.909
PRIMER_RIGHT_46_TM=60.514
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=10.0000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=837
PRIMER_PAIR_47_PENALTY=1.611070
PRIMER_LEFT_47_PENALTY=0.829561
PRIMER_RIGHT_47_PENALTY=0.781508
PRIMER_LEFT_47_SEQUENCE=CCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_47_SEQUENCE=ACGAGGTTGGCAAAAAGAAC
PRIMER_LEFT_47=742,20
PRIMER_RIGHT_47=1571,20
PRIMER_LEFT_47_TM=59.170
PRIMER_RIGHT_47_TM=59.218
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=830
PRIMER_PAIR_48_PENALTY=1.638747
PRIMER_LEFT_48_PENALTY=1.091240
PRIMER_RIGHT_48_PENALTY=0.547508
PRIMER_LEFT_48_SEQUENCE=AGACCCAAAAGAGAAGCCAA
PRIMER_RIGHT_48_SEQUENCE=GGTTGGCAAAAAGAACCAAC
PRIMER_LEFT_48=739,20
PRIMER_RIGHT_48=1567,20
PRIMER_LEFT_48_TM=58.909
PRIMER_RIGHT_48_TM=59.452
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=10.0000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=829
PRIMER_PAIR_49_PENALTY=1.648779
PRIMER_LEFT_49_PENALTY=1.009608
PRIMER_RIGHT_49_PENALTY=0.639172
PRIMER_LEFT_49_SEQUENCE=ACCCAAAAGAGAAGCCAATGT
PRIMER_RIGHT_49_SEQUENCE=CAAACGAGGTTGGCAAAAAG
PRIMER_LEFT_49=741,21
PRIMER_RIGHT_49=1574,20
PRIMER_LEFT_49_TM=59.990
PRIMER_RIGHT_49_TM=60.639
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.6000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=834
=
SEQUENCE_ID=MAGIC.446_2_5129651_5129698_tsu-0_oy-0_328741_23409_TRUE
SEQUENCE_TARGET=950,147
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_14038972_14038982_kn-0_zu-0_12584_5861_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNCTNGATCATGTNNTTAAGCTCNNTGTTCGCCTNAGCAANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACCCGANATGAATGATGNGCNTGGAGTAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGATNGTGTNTTTCCNNGTTTTGAAGCNNNGNAANACGTTGCCCTCTTTCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCGAGNGAAATNAANCCNTNGTTNGAACCTNTAAGCNATTTNGTNTGAGGAACGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTNGATCAGNCTTCTCNCCACTAGGTNGNNGAGAAGGNNNNNNNNNNNNNNNNGAGGAGGAGNACCCATCGATCNAGATGGAGGAGATGATCGNNGAGAGCGATCAGTNCGAGCCNTTGACTTTTCCCTCCAAGGAGAAGAGCTGTTNCGAGCTGGTCTCTCNCTCNNTNTNNNNATNAAGTCAGCGATTGGCTTGCTGCTCAGTCCATCACGGGCTNAAGTCCCGTGTGGTNTGGGAGGANGNGGTGGAAGATGATGGAGTTGTCCAGGAGGACTCATNTNGAGNNNNNNNNNNNNNNNNNNNNNNNNGTCGAGGGTAAACAAATTCCAAAGGGTTTCACCTTCGATGACCACTTTGAAGAANNNNNNNNATTCTTCAGTCGGAAGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAACAGAAGANANATATGNTTNTGCCCTAATTTCTACCAGGTTTNNTGGNCCANTTTGATGGAAGCACTTCGNAAAGGCTNNCAATNGAAGCAANGGNNNNNTTNATTAAGAACTANGGATGGTGNNANNNNNNNTNTTTGTTCGGNAGATNGGANATGAGGTTNNTGTTNGCATTNNNGTANGCNNAGAANTAACCNANTTTCTTCGANNAACTTCTTACNCTNAGAAGCTCNTTCAATTNGGNAACTNCNATTATGTACCCAGCNNCCNCNGCGACGGTNATNANCCCTAGAATAGNTNGGAGAAGGTTGGNGGTTAANTGNGGNAGANCNATTTCAAGAGCNGCCAAGNACNGAACGAGNGCCTNGGGANNAGNANAGGNNAGTCNGNANACCGCGAGGTGAATGAGAAGTCNGGATTAAAGGTGGTTNGATTAAAGGGCCNTTGGGCTGGAANGNGNNNNNCCNGGAAGCNCGAAGTCAATTCAAATTCGAACGTNTGAACANTNAATTCGAAGATCGAAACGGNTNCATTTAAGANNNAATAATTNTGTGTAAATNAGANTTNATTGTAATTCTTAGGTATAANTAATAGGNCCAANNNNNNNAAGAGACACACNCATTTGTTTGCAAACTTANTATACANATTTTCTTACAAAACTCATAGNTTTCTNNATAATCAATAGTTNTTTACACTTATTTTTNTCCTCAANAATAATCCNTCTCANTNNNNAAATAAANNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_6147291_6147339_oy-0_ct-1_25003_13086_TRUE
SEQUENCE_TARGET=950,148
SEQUENCE_TEMPLATE=TGANACCAGCGATTACAGAGATGCAAGATCCTCATGTCCCATCTTCTCCAACTATCTCCTCCGTCTCTTCCTCCGATCTTGATACTGAGGTCAGTTCCTGCAATCTTATACTTAATAGATAATCTAATTAAAGATCAAGNNCTTCATGTGTTTTCTAAGCTACNCNNNNNNNNNNATGCTATAAAGTATCAACCTTTAATTAGAAAACTAGTTTAAAGTNCCAAAGGTTATGTTTTCAGAACTGCCCAGAAACATATATATGTACTTGTTAGAACTTGATTTNNTTTTTTNATTGGTGATGTGAGGATTTAGTCNACAGGATCGTTCTTCCATGACAGAAGCATCACACTTGGAACGCTTATGGGATTTAGTTTCACNGCAACTATGCCGATGCCATTNAGAGCNTCTTCACGCCGCCACGTGTCACCTTCCGTCGCAATCTCACGAGCTTCTAGCTCCAATGCAAGACGCAACCACCAGAGAAAGCGTCCTCCTTCAAATTCGGCTGAGCCTGAGCCGCATCGCCGCCGTAAATGGTGGCGGTTTTGCCGAGATGACGATGACGACGCCGCCGGAAATGGAATCCACCGTGGAACAGGAGATTCAAAGCGGTCATCGCTNGGGGAGTATNTNGAGGTGGAACGGAGGTTTGGAGATGAAGCCGTTNACAACTCCGCCGAGGCAGAGCTAGAGGATGCGGTGGTGGCACGGTACCAAGATCAGCAGCCGGTGATGGGGGAGCGGGCATTGTTCGCTGACGGAAGGGTTCTTCCTCCGGCTTCAGNTGAAGNGGTTACCGGAGAANGNACGCCGGTNGCCACGNCCCTTTGTAGATTTCCGGNNTCTTTAACCNGTATATGCAGCGGTGGNGGAGGNTGAATACGTCGGTTCATGTGTGNGNNNNNNATTTGTATTTAGACTTAAATTTTTGTATNNNNNNNNNNNNNNNNGAATCATTGTTTGTGGAAAATATAACATGTTGNATGTAGTCATTGTCAAANTAATTATTTGAGANNNAANNNNNNNTNNNAAGGAGAATTANNAANNANANTANNNANANNNGNAATANTNNNNNNTNNNCNTNNANNANNNNANACNTGNANANCTNTNGNTNNNAANTGGAGNNNNNCNCTNNATNANNNTNTTNATTTNAAATGNTAANNGTTGNTCTATCANATTGAGTTTTTGCTTCAAANNNNNNNNNNNNNNNNTCTTTATACTAGTCAGTCTTTGGACAATAATTTGACCAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANGAGTGTGCCACATTNGTGGTTANTTTTCCACTAGTATTTCTGAAAGTAAAACAATTAACCACAAATCATCCTCATAACATAAGAGGAGAAGATTCCTAACGACGAAGCAAAATTATCGAACAAGTGGACACACACAAGAAACCGAAAATACGGCAAAATGTTTGTTATGTGTATCCATATTTTATANCAATTGAATTAATAAAATAAACTGTANAAACAAGAAACAATTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAANCCTCGAGTGNTGTTAAAAAAAGGAAGAAGGAAAAAGAATAAAAACTCAAAGGGAACGCAACACACTNAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTNNNCAGCAGCAGCAGNAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGCTGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCACCGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCTGAATGATTTTTTCCTTGGGTAGAGTCAAAATTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.106709
PRIMER_LEFT_0_PENALTY=0.049859
PRIMER_RIGHT_0_PENALTY=0.056850
PRIMER_LEFT_0_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_0_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_0=639,20
PRIMER_RIGHT_0=1444,20
PRIMER_LEFT_0_TM=60.050
PRIMER_RIGHT_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=806
PRIMER_PAIR_1_PENALTY=0.243451
PRIMER_LEFT_1_PENALTY=0.186600
PRIMER_RIGHT_1_PENALTY=0.056850
PRIMER_LEFT_1_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_1_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_1=592,20
PRIMER_RIGHT_1=1444,20
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=853
PRIMER_PAIR_2_PENALTY=0.311463
PRIMER_LEFT_2_PENALTY=0.254613
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_2_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_2=677,20
PRIMER_RIGHT_2=1444,20
PRIMER_LEFT_2_TM=60.255
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=768
PRIMER_PAIR_3_PENALTY=0.410901
PRIMER_LEFT_3_PENALTY=0.254613
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_LEFT_3_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_3_SEQUENCE=GTGTTGCGTTCCCTTTGAGT
PRIMER_LEFT_3=677,20
PRIMER_RIGHT_3=1646,20
PRIMER_LEFT_3_TM=60.255
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=970
PRIMER_PAIR_4_PENALTY=0.422170
PRIMER_LEFT_4_PENALTY=0.365320
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_4_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_4=597,20
PRIMER_RIGHT_4=1444,20
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=848
PRIMER_PAIR_5_PENALTY=0.463402
PRIMER_LEFT_5_PENALTY=0.049859
PRIMER_RIGHT_5_PENALTY=0.413543
PRIMER_LEFT_5_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_5_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_5=639,20
PRIMER_RIGHT_5=1441,20
PRIMER_LEFT_5_TM=60.050
PRIMER_RIGHT_5_TM=59.586
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=803
PRIMER_PAIR_6_PENALTY=0.463402
PRIMER_LEFT_6_PENALTY=0.049859
PRIMER_RIGHT_6_PENALTY=0.413543
PRIMER_LEFT_6_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_6_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_6=639,20
PRIMER_RIGHT_6=1442,20
PRIMER_LEFT_6_TM=60.050
PRIMER_RIGHT_6_TM=59.586
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=804
PRIMER_PAIR_7_PENALTY=0.463402
PRIMER_LEFT_7_PENALTY=0.049859
PRIMER_RIGHT_7_PENALTY=0.413543
PRIMER_LEFT_7_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_7_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_7=639,20
PRIMER_RIGHT_7=1443,20
PRIMER_LEFT_7_TM=60.050
PRIMER_RIGHT_7_TM=59.586
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=805
PRIMER_PAIR_8_PENALTY=0.478724
PRIMER_LEFT_8_PENALTY=0.049859
PRIMER_RIGHT_8_PENALTY=0.428866
PRIMER_LEFT_8_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_8_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_8=639,20
PRIMER_RIGHT_8=1432,20
PRIMER_LEFT_8_TM=60.050
PRIMER_RIGHT_8_TM=59.571
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=794
PRIMER_PAIR_9_PENALTY=0.530362
PRIMER_LEFT_9_PENALTY=0.254613
PRIMER_RIGHT_9_PENALTY=0.275749
PRIMER_LEFT_9_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_9_SEQUENCE=TGCGTTCCCTTTGAGTTTTT
PRIMER_LEFT_9=677,20
PRIMER_RIGHT_9=1642,20
PRIMER_LEFT_9_TM=60.255
PRIMER_RIGHT_9_TM=59.724
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=966
PRIMER_PAIR_10_PENALTY=0.530362
PRIMER_LEFT_10_PENALTY=0.254613
PRIMER_RIGHT_10_PENALTY=0.275749
PRIMER_LEFT_10_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_10_SEQUENCE=TTGCGTTCCCTTTGAGTTTT
PRIMER_LEFT_10=677,20
PRIMER_RIGHT_10=1643,20
PRIMER_LEFT_10_TM=60.255
PRIMER_RIGHT_10_TM=59.724
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=967
PRIMER_PAIR_11_PENALTY=0.530545
PRIMER_LEFT_11_PENALTY=0.473695
PRIMER_RIGHT_11_PENALTY=0.056850
PRIMER_LEFT_11_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_11_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_11=640,20
PRIMER_RIGHT_11=1444,20
PRIMER_LEFT_11_TM=59.526
PRIMER_RIGHT_11_TM=59.943
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=805
PRIMER_PAIR_12_PENALTY=0.567181
PRIMER_LEFT_12_PENALTY=0.510331
PRIMER_RIGHT_12_PENALTY=0.056850
PRIMER_LEFT_12_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_12_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_12=586,20
PRIMER_RIGHT_12=1444,20
PRIMER_LEFT_12_TM=60.510
PRIMER_RIGHT_12_TM=59.943
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=859
PRIMER_PAIR_13_PENALTY=0.600143
PRIMER_LEFT_13_PENALTY=0.186600
PRIMER_RIGHT_13_PENALTY=0.413543
PRIMER_LEFT_13_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_13_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_13=592,20
PRIMER_RIGHT_13=1441,20
PRIMER_LEFT_13_TM=59.813
PRIMER_RIGHT_13_TM=59.586
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=850
PRIMER_PAIR_14_PENALTY=0.600143
PRIMER_LEFT_14_PENALTY=0.186600
PRIMER_RIGHT_14_PENALTY=0.413543
PRIMER_LEFT_14_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_14_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_14=592,20
PRIMER_RIGHT_14=1442,20
PRIMER_LEFT_14_TM=59.813
PRIMER_RIGHT_14_TM=59.586
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=851
PRIMER_PAIR_15_PENALTY=0.600143
PRIMER_LEFT_15_PENALTY=0.186600
PRIMER_RIGHT_15_PENALTY=0.413543
PRIMER_LEFT_15_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_15_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_15=592,20
PRIMER_RIGHT_15=1443,20
PRIMER_LEFT_15_TM=59.813
PRIMER_RIGHT_15_TM=59.586
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=852
PRIMER_PAIR_16_PENALTY=0.615466
PRIMER_LEFT_16_PENALTY=0.186600
PRIMER_RIGHT_16_PENALTY=0.428866
PRIMER_LEFT_16_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_16_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_16=592,20
PRIMER_RIGHT_16=1432,20
PRIMER_LEFT_16_TM=59.813
PRIMER_RIGHT_16_TM=59.571
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=11.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=841
PRIMER_PAIR_17_PENALTY=0.635218
PRIMER_LEFT_17_PENALTY=0.578368
PRIMER_RIGHT_17_PENALTY=0.056850
PRIMER_LEFT_17_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_17_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_17=487,20
PRIMER_RIGHT_17=1444,20
PRIMER_LEFT_17_TM=60.578
PRIMER_RIGHT_17_TM=59.943
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=11.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=958
PRIMER_PAIR_18_PENALTY=0.668156
PRIMER_LEFT_18_PENALTY=0.254613
PRIMER_RIGHT_18_PENALTY=0.413543
PRIMER_LEFT_18_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_18_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_18=677,20
PRIMER_RIGHT_18=1441,20
PRIMER_LEFT_18_TM=60.255
PRIMER_RIGHT_18_TM=59.586
PRIMER_LEFT_18_GC_PERCENT=60.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=765
PRIMER_PAIR_19_PENALTY=0.668156
PRIMER_LEFT_19_PENALTY=0.254613
PRIMER_RIGHT_19_PENALTY=0.413543
PRIMER_LEFT_19_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_19_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_19=677,20
PRIMER_RIGHT_19=1442,20
PRIMER_LEFT_19_TM=60.255
PRIMER_RIGHT_19_TM=59.586
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=766
PRIMER_PAIR_20_PENALTY=0.668156
PRIMER_LEFT_20_PENALTY=0.254613
PRIMER_RIGHT_20_PENALTY=0.413543
PRIMER_LEFT_20_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_20_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_20=677,20
PRIMER_RIGHT_20=1443,20
PRIMER_LEFT_20_TM=60.255
PRIMER_RIGHT_20_TM=59.586
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=767
PRIMER_PAIR_21_PENALTY=0.683479
PRIMER_LEFT_21_PENALTY=0.254613
PRIMER_RIGHT_21_PENALTY=0.428866
PRIMER_LEFT_21_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_21_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_21=677,20
PRIMER_RIGHT_21=1432,20
PRIMER_LEFT_21_TM=60.255
PRIMER_RIGHT_21_TM=59.571
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=756
PRIMER_PAIR_22_PENALTY=0.768641
PRIMER_LEFT_22_PENALTY=0.711791
PRIMER_RIGHT_22_PENALTY=0.056850
PRIMER_LEFT_22_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_22_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_22=594,20
PRIMER_RIGHT_22=1444,20
PRIMER_LEFT_22_TM=59.288
PRIMER_RIGHT_22_TM=59.943
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=851
PRIMER_PAIR_23_PENALTY=0.778863
PRIMER_LEFT_23_PENALTY=0.365320
PRIMER_RIGHT_23_PENALTY=0.413543
PRIMER_LEFT_23_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_23_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_23=597,20
PRIMER_RIGHT_23=1441,20
PRIMER_LEFT_23_TM=59.635
PRIMER_RIGHT_23_TM=59.586
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=845
PRIMER_PAIR_24_PENALTY=0.778863
PRIMER_LEFT_24_PENALTY=0.365320
PRIMER_RIGHT_24_PENALTY=0.413543
PRIMER_LEFT_24_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_24_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_24=597,20
PRIMER_RIGHT_24=1442,20
PRIMER_LEFT_24_TM=59.635
PRIMER_RIGHT_24_TM=59.586
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=846
PRIMER_PAIR_25_PENALTY=0.778863
PRIMER_LEFT_25_PENALTY=0.365320
PRIMER_RIGHT_25_PENALTY=0.413543
PRIMER_LEFT_25_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_25_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_25=597,20
PRIMER_RIGHT_25=1443,20
PRIMER_LEFT_25_TM=59.635
PRIMER_RIGHT_25_TM=59.586
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=847
PRIMER_PAIR_26_PENALTY=0.794186
PRIMER_LEFT_26_PENALTY=0.365320
PRIMER_RIGHT_26_PENALTY=0.428866
PRIMER_LEFT_26_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_26_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_26=597,20
PRIMER_RIGHT_26=1432,20
PRIMER_LEFT_26_TM=59.635
PRIMER_RIGHT_26_TM=59.571
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=836
PRIMER_PAIR_27_PENALTY=0.887238
PRIMER_LEFT_27_PENALTY=0.473695
PRIMER_RIGHT_27_PENALTY=0.413543
PRIMER_LEFT_27_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_27_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_27=640,20
PRIMER_RIGHT_27=1441,20
PRIMER_LEFT_27_TM=59.526
PRIMER_RIGHT_27_TM=59.586
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=802
PRIMER_PAIR_28_PENALTY=0.887238
PRIMER_LEFT_28_PENALTY=0.473695
PRIMER_RIGHT_28_PENALTY=0.413543
PRIMER_LEFT_28_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_28_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_28=640,20
PRIMER_RIGHT_28=1442,20
PRIMER_LEFT_28_TM=59.526
PRIMER_RIGHT_28_TM=59.586
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=803
PRIMER_PAIR_29_PENALTY=0.887238
PRIMER_LEFT_29_PENALTY=0.473695
PRIMER_RIGHT_29_PENALTY=0.413543
PRIMER_LEFT_29_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_29_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_29=640,20
PRIMER_RIGHT_29=1443,20
PRIMER_LEFT_29_TM=59.526
PRIMER_RIGHT_29_TM=59.586
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=804
PRIMER_PAIR_30_PENALTY=0.889843
PRIMER_LEFT_30_PENALTY=0.832993
PRIMER_RIGHT_30_PENALTY=0.056850
PRIMER_LEFT_30_SEQUENCE=CAACCACCAGAGAAAGCGTC
PRIMER_RIGHT_30_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_30=470,20
PRIMER_RIGHT_30=1444,20
PRIMER_LEFT_30_TM=60.833
PRIMER_RIGHT_30_TM=59.943
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=975
PRIMER_PAIR_31_PENALTY=0.889843
PRIMER_LEFT_31_PENALTY=0.832993
PRIMER_RIGHT_31_PENALTY=0.056850
PRIMER_LEFT_31_SEQUENCE=GACGCAACCACCAGAGAAAG
PRIMER_RIGHT_31_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_31=466,20
PRIMER_RIGHT_31=1444,20
PRIMER_LEFT_31_TM=60.833
PRIMER_RIGHT_31_TM=59.943
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=0.889843
PRIMER_LEFT_32_PENALTY=0.832993
PRIMER_RIGHT_32_PENALTY=0.056850
PRIMER_LEFT_32_SEQUENCE=AAGACGCAACCACCAGAGAA
PRIMER_RIGHT_32_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_32=464,20
PRIMER_RIGHT_32=1444,20
PRIMER_LEFT_32_TM=60.833
PRIMER_RIGHT_32_TM=59.943
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=981
PRIMER_PAIR_33_PENALTY=0.902560
PRIMER_LEFT_33_PENALTY=0.473695
PRIMER_RIGHT_33_PENALTY=0.428866
PRIMER_LEFT_33_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_33_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_33=640,20
PRIMER_RIGHT_33=1432,20
PRIMER_LEFT_33_TM=59.526
PRIMER_RIGHT_33_TM=59.571
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=793
PRIMER_PAIR_34_PENALTY=0.919829
PRIMER_LEFT_34_PENALTY=0.862979
PRIMER_RIGHT_34_PENALTY=0.056850
PRIMER_LEFT_34_SEQUENCE=ACGGAGGTTTGGAGATGAAG
PRIMER_RIGHT_34_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_34=641,20
PRIMER_RIGHT_34=1444,20
PRIMER_LEFT_34_TM=59.137
PRIMER_RIGHT_34_TM=59.943
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=804
PRIMER_PAIR_35_PENALTY=0.922928
PRIMER_LEFT_35_PENALTY=0.254613
PRIMER_RIGHT_35_PENALTY=0.668315
PRIMER_LEFT_35_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_35_SEQUENCE=TGTTGCGTTCCCTTTGAGTT
PRIMER_LEFT_35=677,20
PRIMER_RIGHT_35=1645,20
PRIMER_LEFT_35_TM=60.255
PRIMER_RIGHT_35_TM=60.668
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=969
PRIMER_PAIR_36_PENALTY=0.923874
PRIMER_LEFT_36_PENALTY=0.510331
PRIMER_RIGHT_36_PENALTY=0.413543
PRIMER_LEFT_36_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_36_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_36=586,20
PRIMER_RIGHT_36=1441,20
PRIMER_LEFT_36_TM=60.510
PRIMER_RIGHT_36_TM=59.586
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=856
PRIMER_PAIR_37_PENALTY=0.923874
PRIMER_LEFT_37_PENALTY=0.510331
PRIMER_RIGHT_37_PENALTY=0.413543
PRIMER_LEFT_37_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_37_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_37=586,20
PRIMER_RIGHT_37=1442,20
PRIMER_LEFT_37_TM=60.510
PRIMER_RIGHT_37_TM=59.586
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=857
PRIMER_PAIR_38_PENALTY=0.923874
PRIMER_LEFT_38_PENALTY=0.510331
PRIMER_RIGHT_38_PENALTY=0.413543
PRIMER_LEFT_38_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_38_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_38=586,20
PRIMER_RIGHT_38=1443,20
PRIMER_LEFT_38_TM=60.510
PRIMER_RIGHT_38_TM=59.586
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=858
PRIMER_PAIR_39_PENALTY=0.939197
PRIMER_LEFT_39_PENALTY=0.510331
PRIMER_RIGHT_39_PENALTY=0.428866
PRIMER_LEFT_39_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_39_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_39=586,20
PRIMER_RIGHT_39=1432,20
PRIMER_LEFT_39_TM=60.510
PRIMER_RIGHT_39_TM=59.571
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=847
PRIMER_PAIR_40_PENALTY=0.991911
PRIMER_LEFT_40_PENALTY=0.578368
PRIMER_RIGHT_40_PENALTY=0.413543
PRIMER_LEFT_40_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_40_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_40=487,20
PRIMER_RIGHT_40=1441,20
PRIMER_LEFT_40_TM=60.578
PRIMER_RIGHT_40_TM=59.586
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=955
PRIMER_PAIR_41_PENALTY=0.991911
PRIMER_LEFT_41_PENALTY=0.578368
PRIMER_RIGHT_41_PENALTY=0.413543
PRIMER_LEFT_41_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_41_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_41=487,20
PRIMER_RIGHT_41=1442,20
PRIMER_LEFT_41_TM=60.578
PRIMER_RIGHT_41_TM=59.586
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=11.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=956
PRIMER_PAIR_42_PENALTY=0.991911
PRIMER_LEFT_42_PENALTY=0.578368
PRIMER_RIGHT_42_PENALTY=0.413543
PRIMER_LEFT_42_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_42_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_42=487,20
PRIMER_RIGHT_42=1443,20
PRIMER_LEFT_42_TM=60.578
PRIMER_RIGHT_42_TM=59.586
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=11.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=957
PRIMER_PAIR_43_PENALTY=1.007234
PRIMER_LEFT_43_PENALTY=0.578368
PRIMER_RIGHT_43_PENALTY=0.428866
PRIMER_LEFT_43_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_43_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_43=487,20
PRIMER_RIGHT_43=1432,20
PRIMER_LEFT_43_TM=60.578
PRIMER_RIGHT_43_TM=59.571
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=946
PRIMER_PAIR_44_PENALTY=1.036122
PRIMER_LEFT_44_PENALTY=0.254613
PRIMER_RIGHT_44_PENALTY=0.781508
PRIMER_LEFT_44_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_44_SEQUENCE=GTTGCGTTCCCTTTGAGTTT
PRIMER_LEFT_44=677,20
PRIMER_RIGHT_44=1644,20
PRIMER_LEFT_44_TM=60.255
PRIMER_RIGHT_44_TM=59.218
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=968
PRIMER_PAIR_45_PENALTY=1.125334
PRIMER_LEFT_45_PENALTY=0.711791
PRIMER_RIGHT_45_PENALTY=0.413543
PRIMER_LEFT_45_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_45_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_45=594,20
PRIMER_RIGHT_45=1441,20
PRIMER_LEFT_45_TM=59.288
PRIMER_RIGHT_45_TM=59.586
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=848
PRIMER_PAIR_46_PENALTY=1.125334
PRIMER_LEFT_46_PENALTY=0.711791
PRIMER_RIGHT_46_PENALTY=0.413543
PRIMER_LEFT_46_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_46_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_46=594,20
PRIMER_RIGHT_46=1442,20
PRIMER_LEFT_46_TM=59.288
PRIMER_RIGHT_46_TM=59.586
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=849
PRIMER_PAIR_47_PENALTY=1.125334
PRIMER_LEFT_47_PENALTY=0.711791
PRIMER_RIGHT_47_PENALTY=0.413543
PRIMER_LEFT_47_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_47_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_47=594,20
PRIMER_RIGHT_47=1443,20
PRIMER_LEFT_47_TM=59.288
PRIMER_RIGHT_47_TM=59.586
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=850
PRIMER_PAIR_48_PENALTY=1.140657
PRIMER_LEFT_48_PENALTY=0.711791
PRIMER_RIGHT_48_PENALTY=0.428866
PRIMER_LEFT_48_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_48_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_48=594,20
PRIMER_RIGHT_48=1432,20
PRIMER_LEFT_48_TM=59.288
PRIMER_RIGHT_48_TM=59.571
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=839
PRIMER_PAIR_49_PENALTY=1.152585
PRIMER_LEFT_49_PENALTY=1.095734
PRIMER_RIGHT_49_PENALTY=0.056850
PRIMER_LEFT_49_SEQUENCE=CAGAGCTAGAGGATGCGGTG
PRIMER_RIGHT_49_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_49=682,20
PRIMER_RIGHT_49=1444,20
PRIMER_LEFT_49_TM=61.096
PRIMER_RIGHT_49_TM=59.943
PRIMER_LEFT_49_GC_PERCENT=60.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=763
=
SEQUENCE_ID=MAGIC.446_5_18598071_18598179_po-0_zu-0_45881_2330_TRUE
SEQUENCE_TARGET=950,208
SEQUENCE_TEMPLATE=AAACAAAAACATTCAAAAGAACACAATGATAGAGATTTCAAAGTTGTTNACCTTCGGATTCGAGGGACAACGAAGAACGAGATTGAAGAGTAATGATCAAGAACATGAAGCAAGAGAATTGGAACCCNATTGGATTCCATCGACAACCCATTTTAGGTATACACCAATGCTATACAAAATCGCCAGATTTTTTCCTAAATGAACGAATATAAAAATACAAACTTCACAGAATCCTTGGTAGGAGAAGAACGACGAGGTTGATGATTGAACATTCAGGGTCTCTCATTGTTTCCAGTTTTGCAAANTCATAAAACCCTAAAAACAATGTTCATGTCCTAANGAGTCAACAACTCGTACGTTAATTCTCTTCTTCTTGTTAATCCTGGNAAAAAANAAATGCCAACAAGAAACAAACAAACATATATCAAAACTTTAACAACANATGTATACCAATAATNATTAAANCAAAAANNTAAAGAATAAGAATAACCCTCTTGAAGAAGCAATATGAAGAAAACATGGAACCCACCAAANAAAAAAAAACNTTTNTTTTCTCTTGATAAATGAAGAACATTGCAAAATAATTACCTCAAAGAAGAAACTTCTTCACCAAGAATTGGGTATAGGGTTTGAGAAATGAAAAATNAAATANANAAAAAATCCCCAGAAAATAGAAAATGATTATAAAAAAACTTTTAAAAAGATANAATTTCTGTTTAAAAACAAATATTGGATTGATNTTTTCTGATTTCTTGTNGAACTTGCCCTTNTTTTTNCAGGATGATGATGATGAAGATGATGATCTCAGAGTTTTCTCTCTTCGACCATCTTTGGTCTCTCCAGGGCCAAAGGGTGGTGGGTTNNNNNNNNNNNCTCTCTCCTTCCTCTCTCTTTCCTTTTTCTTCCTCTTCTCTCTCTCTTTCTCNTGTCGAATCTTTTTTNCCTATTTTTTAATTCTTTTTTAACAANTTTNCCCCCACAAAACTNTCTCTGTTTTTTTNNTTTTTTNNAAAAATNTCCNATCAATGAGTAAGACCCTATTAACTTCCTTTAGATTTTCCATAANTATAGAAATGTTTGTATTCGCNTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_1952355_1952864_rsch-4_oy-0_46815_677734_TRUE
SEQUENCE_TARGET=950,609
SEQUENCE_TEMPLATE=GTGGGAAAAGGNATATAAACCGTATACCAAAATTTTTGCCANAGGGAGTNTGGNACACTATCAANAGATCCTTCAAAACATGAAGGAAGATGAGCTNNCNANTNAAATCTCATCTCTTTGTCANTATNCATTTGTTNTATATTNANTNCNTAGATTATATTNTTTCAGATTAAGTGGCAGCCTTATAGCAAAGTTGAATTGCNCGAATACACCTCTCAATTGCGTATGGTGTTTGCANAAGNNNCATNCATNNGCTTTAACACCATCNCATANAATTGTCNNGGTACTTTCTNNAANCAATTAGGACNGGAGGAGTCAGACATACGGNTGCCACCNGAAATAAAGCCGAANNNGNCGTCTAAGTTAAGCAGACACAAAGACAAGNACTGGAGAGAGCATAANAGTTTCTACTCACANATGAATTTGGCNTGGGAAAANCGAGATAATCGTTTGGTGTATCAACGACACAATNAGGAATNTGAGGNGAGGTGATTTTAATNTTTTTTTNNAGTCGATTGTGTTTACATGTAGGTAGGAGGAAACTNGTTTTTCTTTTTTAAACACAAGTGCTTCATGAANCACATGTGTTTTAGATAATGTGTTTNTTTTTCTAACAGAAGTGCCTCATGGAATCCACTTGGTCTATACTGANTNTTTTTTAACACAACTGCCTCATGAAACACCTGNNCGTTATATAATGTGNTTGTTTTTCTAACANAAGTGCCTCATGGAACCCGCTNGGTCTATATATTTANGTTTTTTNATGATTTTTTNTTTTGTTTTGATAGATGAATTTNGGCTNCCATTCCCGGTCTATCTCANGAAGAGGTGGAGTTGAGGCCATCACCAATGATNCGGTATTTGTTGTNTTTANATTTTTTTATCTTTTTATCNCNAAATGTTTNTATATTAAACTTATATAAATGGTGAATCTGGATAGGTTGATAATCAAGATGAATCAGATGAAATCCCTTTCCCTATCTCAGTTGAGAACNTCACANGTGCCANCAACAATGATCAGGTATTTGTTGTGTTTAAAGTTTTATCTTTTTATCTCCAAATGTTTATTTAATAAACTTATATAAATGGTGAGTAAGTCAATTGGGATTTANCTNTGGTTTTTCTTGAATTGTGTTTATGATTATTTGTTTTGTTTGATAGATGAATCTCGANGCCCNTNNGCCNNNNNNATGATACGTTTTAGAACACAAACTATAGCAAAAGCAATGTAAATGGAANNAANTTTATTGATAAAAGAAAGATACANGTTTTAAGNGNTNTGCNTNGNGNCNCTTCNCNCCCCCTTACACCTTGTTTTCACTCANATACAAGATAAACCCTATCTCTCTCTTCNTATTTTCTTATATAGTACTATCTGCCCTCATCATCTCACAGAGCACCNTTACAGTNATAAGCAATGAGAATGTGACAGCTAATAACTATGATTNAACCTTNACTCTAGTANAGTTCACCTTAANTTCTAGAGTCTTCNTTTGGTTCCTTCTTCTTNCTCTTTCTNNTGTTNGGTCTTGGGCCTTCTTTTATATTGCNNTNGAATAGGNGGGTGNTGGGNCTCATACGTANNNNTNTNNNNNCTGNAGACCAGTCNATCTCATGTANAGTAGAGGCCAGCAATAGTGATCAGGTATTTGTTGTGTTTAAAGTTNTNTNTTTTTATCTCCANATGTNTATATATTGTTTTGTGTNTATGGATTTAACNAATCTAGACCAATGGATAAANNTTTTTTTTATNATNTTTTGTTTTGTTTTGATAGATGAATTNNGGCTCCTANTTCCAGTCTATCTCNGGCAAAGGTAGAGTAGAGGCCAGCACCAAAGATCCGGTATTTGTTGTGTTTAAAGTTTTATCTTTTTATCTCNAAANGTTTATATNTTAAACTTATATAAATGGTGAATCTGGATAGGTTGATGATCAAGGTCCATCAGATGAAATTNATTCCCATAATGGCCCCTTATATATNAATGATGAGGAGGGAGCGAATGAAACGGTTTGTCATTCTCAATAATGCAAATGAATTTGGTTTTGTAACATATATTCTAGGATATTAGGAAACTAAATATNAATAACTNGCAGGAAAGAAGCTATCCTCGACCTCTTCTTAAAAGAGGAAGAGACGGAATACTTAGAAGTGAAGGAAAATTAAAACAAAGGTTATATTTACTCCATCTTCTCGCACCAGAAAAATGCAGGGGAACAAGAAACAAAAATACCGCTTGATAGCCNCGATTTTGAAGGAAACGAAGCCAAAAGGAAAANAAAATACAAATCAACCTTCTTCAATAGTTTANTTTTCTTATGTTCTTGTTACGTTTTTATTCAGATNTTTATTCAGTTNAGAAACNAATTCNNTGGAGTCTTTNATTAGTTTTATCAATNNNNNNNNNNNNNNNNTANAATTTATAACTTCGTATCCAAATTCCTNAANCAGTGACTTGCCTGATGAATTGCTGGTGGAGATATTAACGTTTCTTCCAACAAAAGNTGTTG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.061795
PRIMER_LEFT_0_PENALTY=4.049325
PRIMER_RIGHT_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_0_SEQUENCE=TTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_0=924,20
PRIMER_RIGHT_0=1836,20
PRIMER_LEFT_0_TM=55.951
PRIMER_RIGHT_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=913
PRIMER_PAIR_1_PENALTY=4.083172
PRIMER_LEFT_1_PENALTY=4.070702
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_1_SEQUENCE=TTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_1=922,22
PRIMER_RIGHT_1=1836,20
PRIMER_LEFT_1_TM=57.929
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=915
PRIMER_PAIR_2_PENALTY=4.094157
PRIMER_LEFT_2_PENALTY=4.049325
PRIMER_RIGHT_2_PENALTY=0.044832
PRIMER_LEFT_2_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_2_SEQUENCE=ATACCGGATCTTTGGTGCTG
PRIMER_LEFT_2=924,20
PRIMER_RIGHT_2=1847,20
PRIMER_LEFT_2_TM=55.951
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=924
PRIMER_PAIR_3_PENALTY=4.115533
PRIMER_LEFT_3_PENALTY=4.070702
PRIMER_RIGHT_3_PENALTY=0.044832
PRIMER_LEFT_3_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_3_SEQUENCE=ATACCGGATCTTTGGTGCTG
PRIMER_LEFT_3=922,22
PRIMER_RIGHT_3=1847,20
PRIMER_LEFT_3_TM=57.929
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=926
PRIMER_PAIR_4_PENALTY=4.446269
PRIMER_LEFT_4_PENALTY=4.049325
PRIMER_RIGHT_4_PENALTY=0.396944
PRIMER_LEFT_4_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_4_SEQUENCE=TTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_4=924,20
PRIMER_RIGHT_4=1837,20
PRIMER_LEFT_4_TM=55.951
PRIMER_RIGHT_4_TM=60.397
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=914
PRIMER_PAIR_5_PENALTY=4.467645
PRIMER_LEFT_5_PENALTY=4.070702
PRIMER_RIGHT_5_PENALTY=0.396944
PRIMER_LEFT_5_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_5_SEQUENCE=TTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_5=922,22
PRIMER_RIGHT_5=1837,20
PRIMER_LEFT_5_TM=57.929
PRIMER_RIGHT_5_TM=60.397
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=5.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=916
PRIMER_PAIR_6_PENALTY=4.553021
PRIMER_LEFT_6_PENALTY=4.049325
PRIMER_RIGHT_6_PENALTY=0.503696
PRIMER_LEFT_6_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_6_SEQUENCE=CTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_6=924,20
PRIMER_RIGHT_6=1838,20
PRIMER_LEFT_6_TM=55.951
PRIMER_RIGHT_6_TM=59.496
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=5.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=915
PRIMER_PAIR_7_PENALTY=4.556018
PRIMER_LEFT_7_PENALTY=4.543548
PRIMER_RIGHT_7_PENALTY=0.012470
PRIMER_LEFT_7_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_7_SEQUENCE=TTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_7=923,21
PRIMER_RIGHT_7=1836,20
PRIMER_LEFT_7_TM=56.456
PRIMER_RIGHT_7_TM=60.012
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=914
PRIMER_PAIR_8_PENALTY=4.574398
PRIMER_LEFT_8_PENALTY=4.070702
PRIMER_RIGHT_8_PENALTY=0.503696
PRIMER_LEFT_8_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_8_SEQUENCE=CTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_8=922,22
PRIMER_RIGHT_8=1838,20
PRIMER_LEFT_8_TM=57.929
PRIMER_RIGHT_8_TM=59.496
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=5.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=917
PRIMER_PAIR_9_PENALTY=4.588379
PRIMER_LEFT_9_PENALTY=4.543548
PRIMER_RIGHT_9_PENALTY=0.044832
PRIMER_LEFT_9_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_9_SEQUENCE=ATACCGGATCTTTGGTGCTG
PRIMER_LEFT_9=923,21
PRIMER_RIGHT_9=1847,20
PRIMER_LEFT_9_TM=56.456
PRIMER_RIGHT_9_TM=59.955
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=925
PRIMER_PAIR_10_PENALTY=4.940491
PRIMER_LEFT_10_PENALTY=4.543548
PRIMER_RIGHT_10_PENALTY=0.396944
PRIMER_LEFT_10_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_10_SEQUENCE=TTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_10=923,21
PRIMER_RIGHT_10=1837,20
PRIMER_LEFT_10_TM=56.456
PRIMER_RIGHT_10_TM=60.397
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=5.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=915
PRIMER_PAIR_11_PENALTY=5.047244
PRIMER_LEFT_11_PENALTY=4.543548
PRIMER_RIGHT_11_PENALTY=0.503696
PRIMER_LEFT_11_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_11_SEQUENCE=CTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_11=923,21
PRIMER_RIGHT_11=1838,20
PRIMER_LEFT_11_TM=56.456
PRIMER_RIGHT_11_TM=59.496
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=5.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=916
PRIMER_PAIR_12_PENALTY=5.198323
PRIMER_LEFT_12_PENALTY=4.049325
PRIMER_RIGHT_12_PENALTY=1.148998
PRIMER_LEFT_12_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_12_SEQUENCE=TGATCACTATTGCTGGCCTCT
PRIMER_LEFT_12=924,20
PRIMER_RIGHT_12=1643,21
PRIMER_LEFT_12_TM=55.951
PRIMER_RIGHT_12_TM=59.851
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=720
PRIMER_PAIR_13_PENALTY=5.198323
PRIMER_LEFT_13_PENALTY=4.049325
PRIMER_RIGHT_13_PENALTY=1.148998
PRIMER_LEFT_13_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_13_SEQUENCE=CTGATCACTATTGCTGGCCTC
PRIMER_LEFT_13=924,20
PRIMER_RIGHT_13=1644,21
PRIMER_LEFT_13_TM=55.951
PRIMER_RIGHT_13_TM=59.851
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=721
PRIMER_PAIR_14_PENALTY=5.200429
PRIMER_LEFT_14_PENALTY=4.049325
PRIMER_RIGHT_14_PENALTY=1.151103
PRIMER_LEFT_14_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_14_SEQUENCE=AAATACCGGATCTTTGGTGCT
PRIMER_LEFT_14=924,20
PRIMER_RIGHT_14=1849,21
PRIMER_LEFT_14_TM=55.951
PRIMER_RIGHT_14_TM=59.849
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=926
PRIMER_PAIR_15_PENALTY=5.219700
PRIMER_LEFT_15_PENALTY=4.070702
PRIMER_RIGHT_15_PENALTY=1.148998
PRIMER_LEFT_15_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_15_SEQUENCE=TGATCACTATTGCTGGCCTCT
PRIMER_LEFT_15=922,22
PRIMER_RIGHT_15=1643,21
PRIMER_LEFT_15_TM=57.929
PRIMER_RIGHT_15_TM=59.851
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=722
PRIMER_PAIR_16_PENALTY=5.219700
PRIMER_LEFT_16_PENALTY=4.070702
PRIMER_RIGHT_16_PENALTY=1.148998
PRIMER_LEFT_16_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_16_SEQUENCE=CTGATCACTATTGCTGGCCTC
PRIMER_LEFT_16=922,22
PRIMER_RIGHT_16=1644,21
PRIMER_LEFT_16_TM=57.929
PRIMER_RIGHT_16_TM=59.851
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=723
PRIMER_PAIR_17_PENALTY=5.221805
PRIMER_LEFT_17_PENALTY=4.070702
PRIMER_RIGHT_17_PENALTY=1.151103
PRIMER_LEFT_17_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_17_SEQUENCE=AAATACCGGATCTTTGGTGCT
PRIMER_LEFT_17=922,22
PRIMER_RIGHT_17=1849,21
PRIMER_LEFT_17_TM=57.929
PRIMER_RIGHT_17_TM=59.849
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=928
PRIMER_PAIR_18_PENALTY=5.223202
PRIMER_LEFT_18_PENALTY=4.049325
PRIMER_RIGHT_18_PENALTY=1.173876
PRIMER_LEFT_18_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_18_SEQUENCE=TGATCACTATTGCTGGCCTC
PRIMER_LEFT_18=924,20
PRIMER_RIGHT_18=1643,20
PRIMER_LEFT_18_TM=55.951
PRIMER_RIGHT_18_TM=58.826
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=720
PRIMER_PAIR_19_PENALTY=5.244578
PRIMER_LEFT_19_PENALTY=4.070702
PRIMER_RIGHT_19_PENALTY=1.173876
PRIMER_LEFT_19_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_19_SEQUENCE=TGATCACTATTGCTGGCCTC
PRIMER_LEFT_19=922,22
PRIMER_RIGHT_19=1643,20
PRIMER_LEFT_19_TM=57.929
PRIMER_RIGHT_19_TM=58.826
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=722
PRIMER_PAIR_20_PENALTY=5.519782
PRIMER_LEFT_20_PENALTY=4.049325
PRIMER_RIGHT_20_PENALTY=1.470456
PRIMER_LEFT_20_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_20_SEQUENCE=AATACCGGATCTTTGGTGCT
PRIMER_LEFT_20=924,20
PRIMER_RIGHT_20=1848,20
PRIMER_LEFT_20_TM=55.951
PRIMER_RIGHT_20_TM=58.530
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=925
PRIMER_PAIR_21_PENALTY=5.538619
PRIMER_LEFT_21_PENALTY=4.049325
PRIMER_RIGHT_21_PENALTY=1.489294
PRIMER_LEFT_21_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_21_SEQUENCE=TGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_21=924,20
PRIMER_RIGHT_21=1835,21
PRIMER_LEFT_21_TM=55.951
PRIMER_RIGHT_21_TM=59.511
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=57.143
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=5.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=912
PRIMER_PAIR_22_PENALTY=5.541158
PRIMER_LEFT_22_PENALTY=4.070702
PRIMER_RIGHT_22_PENALTY=1.470456
PRIMER_LEFT_22_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_22_SEQUENCE=AATACCGGATCTTTGGTGCT
PRIMER_LEFT_22=922,22
PRIMER_RIGHT_22=1848,20
PRIMER_LEFT_22_TM=57.929
PRIMER_RIGHT_22_TM=58.530
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=927
PRIMER_PAIR_23_PENALTY=5.559995
PRIMER_LEFT_23_PENALTY=4.070702
PRIMER_RIGHT_23_PENALTY=1.489294
PRIMER_LEFT_23_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_23_SEQUENCE=TGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_23=922,22
PRIMER_RIGHT_23=1835,21
PRIMER_LEFT_23_TM=57.929
PRIMER_RIGHT_23_TM=59.511
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=57.143
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=5.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=914
PRIMER_PAIR_24_PENALTY=5.692546
PRIMER_LEFT_24_PENALTY=4.543548
PRIMER_RIGHT_24_PENALTY=1.148998
PRIMER_LEFT_24_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_24_SEQUENCE=TGATCACTATTGCTGGCCTCT
PRIMER_LEFT_24=923,21
PRIMER_RIGHT_24=1643,21
PRIMER_LEFT_24_TM=56.456
PRIMER_RIGHT_24_TM=59.851
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=721
PRIMER_PAIR_25_PENALTY=5.692546
PRIMER_LEFT_25_PENALTY=4.543548
PRIMER_RIGHT_25_PENALTY=1.148998
PRIMER_LEFT_25_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_25_SEQUENCE=CTGATCACTATTGCTGGCCTC
PRIMER_LEFT_25=923,21
PRIMER_RIGHT_25=1644,21
PRIMER_LEFT_25_TM=56.456
PRIMER_RIGHT_25_TM=59.851
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=52.381
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=722
PRIMER_PAIR_26_PENALTY=5.694651
PRIMER_LEFT_26_PENALTY=4.543548
PRIMER_RIGHT_26_PENALTY=1.151103
PRIMER_LEFT_26_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_26_SEQUENCE=AAATACCGGATCTTTGGTGCT
PRIMER_LEFT_26=923,21
PRIMER_RIGHT_26=1849,21
PRIMER_LEFT_26_TM=56.456
PRIMER_RIGHT_26_TM=59.849
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=927
PRIMER_PAIR_27_PENALTY=5.717424
PRIMER_LEFT_27_PENALTY=4.543548
PRIMER_RIGHT_27_PENALTY=1.173876
PRIMER_LEFT_27_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_27_SEQUENCE=TGATCACTATTGCTGGCCTC
PRIMER_LEFT_27=923,21
PRIMER_RIGHT_27=1643,20
PRIMER_LEFT_27_TM=56.456
PRIMER_RIGHT_27_TM=58.826
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=721
PRIMER_PAIR_28_PENALTY=5.751487
PRIMER_LEFT_28_PENALTY=4.049325
PRIMER_RIGHT_28_PENALTY=1.702162
PRIMER_LEFT_28_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_28_SEQUENCE=GATCTTTGGTGCTGGCCTCT
PRIMER_LEFT_28=924,20
PRIMER_RIGHT_28=1841,20
PRIMER_LEFT_28_TM=55.951
PRIMER_RIGHT_28_TM=61.702
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=918
PRIMER_PAIR_29_PENALTY=5.772863
PRIMER_LEFT_29_PENALTY=4.070702
PRIMER_RIGHT_29_PENALTY=1.702162
PRIMER_LEFT_29_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_29_SEQUENCE=GATCTTTGGTGCTGGCCTCT
PRIMER_LEFT_29=922,22
PRIMER_RIGHT_29=1841,20
PRIMER_LEFT_29_TM=57.929
PRIMER_RIGHT_29_TM=61.702
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=920
PRIMER_PAIR_30_PENALTY=6.014004
PRIMER_LEFT_30_PENALTY=4.543548
PRIMER_RIGHT_30_PENALTY=1.470456
PRIMER_LEFT_30_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_30_SEQUENCE=AATACCGGATCTTTGGTGCT
PRIMER_LEFT_30=923,21
PRIMER_RIGHT_30=1848,20
PRIMER_LEFT_30_TM=56.456
PRIMER_RIGHT_30_TM=58.530
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=926
PRIMER_PAIR_31_PENALTY=6.032841
PRIMER_LEFT_31_PENALTY=4.543548
PRIMER_RIGHT_31_PENALTY=1.489294
PRIMER_LEFT_31_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_31_SEQUENCE=TGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_31=923,21
PRIMER_RIGHT_31=1835,21
PRIMER_LEFT_31_TM=56.456
PRIMER_RIGHT_31_TM=59.511
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=57.143
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=5.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=913
PRIMER_PAIR_32_PENALTY=6.245709
PRIMER_LEFT_32_PENALTY=4.543548
PRIMER_RIGHT_32_PENALTY=1.702162
PRIMER_LEFT_32_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_32_SEQUENCE=GATCTTTGGTGCTGGCCTCT
PRIMER_LEFT_32=923,21
PRIMER_RIGHT_32=1841,20
PRIMER_LEFT_32_TM=56.456
PRIMER_RIGHT_32_TM=61.702
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=919
PRIMER_PAIR_33_PENALTY=6.281290
PRIMER_LEFT_33_PENALTY=4.049325
PRIMER_RIGHT_33_PENALTY=2.231965
PRIMER_LEFT_33_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_33_SEQUENCE=AATACCGGATCTTTGGTGCTG
PRIMER_LEFT_33=924,20
PRIMER_RIGHT_33=1848,21
PRIMER_LEFT_33_TM=55.951
PRIMER_RIGHT_33_TM=61.232
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=925
PRIMER_PAIR_34_PENALTY=6.302666
PRIMER_LEFT_34_PENALTY=4.070702
PRIMER_RIGHT_34_PENALTY=2.231965
PRIMER_LEFT_34_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_34_SEQUENCE=AATACCGGATCTTTGGTGCTG
PRIMER_LEFT_34=922,22
PRIMER_RIGHT_34=1848,21
PRIMER_LEFT_34_TM=57.929
PRIMER_RIGHT_34_TM=61.232
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=927
PRIMER_PAIR_35_PENALTY=6.365366
PRIMER_LEFT_35_PENALTY=4.049325
PRIMER_RIGHT_35_PENALTY=2.316040
PRIMER_LEFT_35_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_35_SEQUENCE=TTTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_35=924,20
PRIMER_RIGHT_35=1837,21
PRIMER_LEFT_35_TM=55.951
PRIMER_RIGHT_35_TM=61.316
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=914
PRIMER_PAIR_36_PENALTY=6.365366
PRIMER_LEFT_36_PENALTY=4.049325
PRIMER_RIGHT_36_PENALTY=2.316040
PRIMER_LEFT_36_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_36_SEQUENCE=CTTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_36=924,20
PRIMER_RIGHT_36=1838,21
PRIMER_LEFT_36_TM=55.951
PRIMER_RIGHT_36_TM=61.316
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=57.143
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=5.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=915
PRIMER_PAIR_37_PENALTY=6.365366
PRIMER_LEFT_37_PENALTY=4.049325
PRIMER_RIGHT_37_PENALTY=2.316040
PRIMER_LEFT_37_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_37_SEQUENCE=TCTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_37=924,20
PRIMER_RIGHT_37=1839,21
PRIMER_LEFT_37_TM=55.951
PRIMER_RIGHT_37_TM=61.316
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=5.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=916
PRIMER_PAIR_38_PENALTY=6.386742
PRIMER_LEFT_38_PENALTY=4.070702
PRIMER_RIGHT_38_PENALTY=2.316040
PRIMER_LEFT_38_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_38_SEQUENCE=TTTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_38=922,22
PRIMER_RIGHT_38=1837,21
PRIMER_LEFT_38_TM=57.929
PRIMER_RIGHT_38_TM=61.316
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=916
PRIMER_PAIR_39_PENALTY=6.386742
PRIMER_LEFT_39_PENALTY=4.070702
PRIMER_RIGHT_39_PENALTY=2.316040
PRIMER_LEFT_39_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_39_SEQUENCE=CTTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_39=922,22
PRIMER_RIGHT_39=1838,21
PRIMER_LEFT_39_TM=57.929
PRIMER_RIGHT_39_TM=61.316
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=57.143
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=5.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=917
PRIMER_PAIR_40_PENALTY=6.386742
PRIMER_LEFT_40_PENALTY=4.070702
PRIMER_RIGHT_40_PENALTY=2.316040
PRIMER_LEFT_40_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_40_SEQUENCE=TCTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_40=922,22
PRIMER_RIGHT_40=1839,21
PRIMER_LEFT_40_TM=57.929
PRIMER_RIGHT_40_TM=61.316
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=5.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=918
PRIMER_PAIR_41_PENALTY=6.484526
PRIMER_LEFT_41_PENALTY=4.049325
PRIMER_RIGHT_41_PENALTY=2.435201
PRIMER_LEFT_41_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_41_SEQUENCE=GCTGGCCTCTACTCTACCTTTG
PRIMER_LEFT_41=924,20
PRIMER_RIGHT_41=1831,22
PRIMER_LEFT_41_TM=55.951
PRIMER_RIGHT_41_TM=59.565
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=54.545
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.3000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=908
PRIMER_PAIR_42_PENALTY=6.505902
PRIMER_LEFT_42_PENALTY=4.070702
PRIMER_RIGHT_42_PENALTY=2.435201
PRIMER_LEFT_42_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_42_SEQUENCE=GCTGGCCTCTACTCTACCTTTG
PRIMER_LEFT_42=922,22
PRIMER_RIGHT_42=1831,22
PRIMER_LEFT_42_TM=57.929
PRIMER_RIGHT_42_TM=59.565
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=54.545
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=910
PRIMER_PAIR_43_PENALTY=6.775512
PRIMER_LEFT_43_PENALTY=4.543548
PRIMER_RIGHT_43_PENALTY=2.231965
PRIMER_LEFT_43_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_43_SEQUENCE=AATACCGGATCTTTGGTGCTG
PRIMER_LEFT_43=923,21
PRIMER_RIGHT_43=1848,21
PRIMER_LEFT_43_TM=56.456
PRIMER_RIGHT_43_TM=61.232
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=926
PRIMER_PAIR_44_PENALTY=6.809647
PRIMER_LEFT_44_PENALTY=4.049325
PRIMER_RIGHT_44_PENALTY=2.760322
PRIMER_LEFT_44_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_44_SEQUENCE=TGCTGGCCTCTACTCTACCTT
PRIMER_LEFT_44=924,20
PRIMER_RIGHT_44=1832,21
PRIMER_LEFT_44_TM=55.951
PRIMER_RIGHT_44_TM=58.240
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=909
PRIMER_PAIR_45_PENALTY=6.831023
PRIMER_LEFT_45_PENALTY=4.070702
PRIMER_RIGHT_45_PENALTY=2.760322
PRIMER_LEFT_45_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_45_SEQUENCE=TGCTGGCCTCTACTCTACCTT
PRIMER_LEFT_45=922,22
PRIMER_RIGHT_45=1832,21
PRIMER_LEFT_45_TM=57.929
PRIMER_RIGHT_45_TM=58.240
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=911
PRIMER_PAIR_46_PENALTY=6.831637
PRIMER_LEFT_46_PENALTY=4.049325
PRIMER_RIGHT_46_PENALTY=2.782312
PRIMER_LEFT_46_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_46_SEQUENCE=CTGATCACTATTGCTGGCCTCT
PRIMER_LEFT_46=924,20
PRIMER_RIGHT_46=1644,22
PRIMER_LEFT_46_TM=55.951
PRIMER_RIGHT_46_TM=60.782
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=721
PRIMER_PAIR_47_PENALTY=6.853014
PRIMER_LEFT_47_PENALTY=4.070702
PRIMER_RIGHT_47_PENALTY=2.782312
PRIMER_LEFT_47_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_47_SEQUENCE=CTGATCACTATTGCTGGCCTCT
PRIMER_LEFT_47=922,22
PRIMER_RIGHT_47=1644,22
PRIMER_LEFT_47_TM=57.929
PRIMER_RIGHT_47_TM=60.782
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=723
PRIMER_PAIR_48_PENALTY=6.859588
PRIMER_LEFT_48_PENALTY=4.543548
PRIMER_RIGHT_48_PENALTY=2.316040
PRIMER_LEFT_48_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_48_SEQUENCE=TTTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_48=923,21
PRIMER_RIGHT_48=1837,21
PRIMER_LEFT_48_TM=56.456
PRIMER_RIGHT_48_TM=61.316
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.1000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=915
PRIMER_PAIR_49_PENALTY=6.859588
PRIMER_LEFT_49_PENALTY=4.543548
PRIMER_RIGHT_49_PENALTY=2.316040
PRIMER_LEFT_49_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_49_SEQUENCE=CTTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_49=923,21
PRIMER_RIGHT_49=1838,21
PRIMER_LEFT_49_TM=56.456
PRIMER_RIGHT_49_TM=61.316
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=57.143
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=5.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=916
=
SEQUENCE_ID=MAGIC.105_1_16485246_16485261_can-0_bur-0_670291_78_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=GTACTTACCTGCCCCTCTCGACAAAATTTCTTNTGTACTTTCACATTTCTNTGTAATAAACTCGGTAGGTTTGCGAAAACCTNGCNGCCGGNAAAAAAAAAAATCAATGGAAGGAGGAGGNAGAGNACCAAATCAAACGATTCTCAGTGAAATAGAACATATGCCTGAAGCTCCACGTCAACGTATCTCTCATCACCGTCGAGCTCGCTCTGAAACCTTCTTCTCCGGCGAATCAATCGACGATCTCCTCTTATTCGATCCTTCCGANATCGATTTCTCTTCTCTAGACTTCCTCAACGCTCCACCACCACCACNACAATCACAACAACAACCNCAAGCTTCTCCCATGTCCGTTGATTCNGAAGAAACCTCATCNAACGGTGTTGTTCCTCCNAANNCTCTTCCTCCNAAACCCGAAGCTAGATTCGGTCGCCATGTTCGTAGCTTCTCGGTTGATTNCGATTTCTTCGATGATTTGGGTGTTACTGAGGAGAAGTNTNTAGCTACAAGTTCAGGAGAGAAGAAGAAAGGGNATCATCATCATAGCAGGAGTAATTCTATGGATGGAGAGATGAGTTCGGCGTCGTTTAATATCGAATCGATTTTAGCTTCTGTGAGTGGTAAAGATAGTGGGAAGAAGAATATGGGTATGGGTGGTGATAGACTTGCTGAGCTTGCTTTGCTTGATCCTAAAAGAGCTAAAAGGTATNTAATTTAGTGTTGNNNTTTACTTAAANNTTGTAACTTNTTTGNGATTTGATTGAGGTTTGAANNAGTGTTTTNNGNTGNNNTTTTGATAGGATTTTAGCGAATAGACAATCTGCGGCGAGGTCGAAAGAGAGGAAGATTAGGTATACTGGTGAGTTAGAGAGGAAGGTTCAGACACTTCAGAATGAAGCTACTACATTGTCTGCTCAAGTNACTATGTTACAGGTAATGTTTTNTATTACTTNGTCATNGTGTTTATGCAATTGGTGTGACTAGTGAAGGTTGGTGAGTANATTACNNNNNNTCTNNNNANCTNTNTNNNNNNNNNNNCTGATNTNNTNANTNNAGNTTNNNNNNNNGNACTNNNNNNNNTNNCTTGATTNGNNCNTGGCAGCTTNTANGCTTGTANTGNTGCTTAGTNTGTTTAAGATTGCTTCTTNGTGTNTAGAGAGGACATGTGTTTGCTACTAGNNNNNNNNATAGATGCAGNNTTATAAGATATNNTATTNTNATGTTAATTTAGCNNATNAATCAACATGTGAGATATGTCANTAGTGTAATGTATACANTTGCAAGAGTGTAANNTTTGTTTNCACTTTAGTGATAAAAGCAACATAGGAGTAAGATNAAATGATTCATGTNTCTTGCATCTCTAATCTGTGCTTTTTCTTATATTGTGTAGAGAGGAACATCAGAGCTGAACACTGAAAATAAACACCTCAAAATGCNGCTTCAAGCTTTAGAGCAACAAGCTGAACTTAGGGATGGTNAAGCCATTTTCTTNTTCATTGGNTCGTTCTCAATATGTANACCNAAGTCNNCNTTACGATATNTATTGTTCGGTGTAATTTTCAGCTTTGAATGAAGCNCTGCGGGATGAACTNAACCGACTTAAGGTGGTAGCTGGAGAAATTCCTCAGGGGAANGGAAATTCNTACAACCGTGCTCAATTCTCATCTCAGCAATCGGCAATGAATCAGTTTGGGAACAANANGAACCANCAGATGAGTACAAACGGGCAGCCATCGCTCCCAAGCTACATGGATTTCACCAAGAGAGGCTGAGTTCGTGTCACCTATATATGTTTGCTGAGTTATAAATACGTTATATTCATTTCGGGCTGCAATATTTGATGTATGTAAAAAGTATGTATGTATCCATGTATNATGGCTTTTGNNNTGTTANCGTCTATGCAGATATGTTCATGGCGANATCGGTCTATNTTTACNAGTAGTATCCATTGTAATATACTANCCAAAAATCTTTATAGTNAGCACTTTTCCTAGGCATTAAGCACCGATCATCAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.508608
PRIMER_LEFT_0_PENALTY=0.841762
PRIMER_RIGHT_0_PENALTY=0.666846
PRIMER_LEFT_0_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_0_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_0=826,20
PRIMER_RIGHT_0=1730,20
PRIMER_LEFT_0_TM=59.158
PRIMER_RIGHT_0_TM=60.667
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=905
PRIMER_PAIR_1_PENALTY=1.684734
PRIMER_LEFT_1_PENALTY=0.841762
PRIMER_RIGHT_1_PENALTY=0.842972
PRIMER_LEFT_1_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_1_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_1=826,20
PRIMER_RIGHT_1=1694,20
PRIMER_LEFT_1_TM=59.158
PRIMER_RIGHT_1_TM=60.843
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=869
PRIMER_PAIR_2_PENALTY=1.714406
PRIMER_LEFT_2_PENALTY=0.841762
PRIMER_RIGHT_2_PENALTY=0.872644
PRIMER_LEFT_2_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_2_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_2=826,20
PRIMER_RIGHT_2=1783,20
PRIMER_LEFT_2_TM=59.158
PRIMER_RIGHT_2_TM=60.873
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=958
PRIMER_PAIR_3_PENALTY=1.823411
PRIMER_LEFT_3_PENALTY=0.777580
PRIMER_RIGHT_3_PENALTY=1.045831
PRIMER_LEFT_3_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_3_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_3=671,20
PRIMER_RIGHT_3=1623,21
PRIMER_LEFT_3_TM=59.222
PRIMER_RIGHT_3_TM=59.954
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=953
PRIMER_PAIR_4_PENALTY=1.887594
PRIMER_LEFT_4_PENALTY=0.841762
PRIMER_RIGHT_4_PENALTY=1.045831
PRIMER_LEFT_4_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_4_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_4=826,20
PRIMER_RIGHT_4=1623,21
PRIMER_LEFT_4_TM=59.158
PRIMER_RIGHT_4_TM=59.954
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=798
PRIMER_PAIR_5_PENALTY=1.960235
PRIMER_LEFT_5_PENALTY=0.841762
PRIMER_RIGHT_5_PENALTY=1.118473
PRIMER_LEFT_5_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_5_SEQUENCE=TAGGTGACACGAACTCAGCC
PRIMER_LEFT_5=826,20
PRIMER_RIGHT_5=1785,20
PRIMER_LEFT_5_TM=59.158
PRIMER_RIGHT_5_TM=58.882
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=960
PRIMER_PAIR_6_PENALTY=1.982603
PRIMER_LEFT_6_PENALTY=0.841762
PRIMER_RIGHT_6_PENALTY=1.140841
PRIMER_LEFT_6_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_6_SEQUENCE=CTGATTCATTGCCGATTGCT
PRIMER_LEFT_6=826,20
PRIMER_RIGHT_6=1687,20
PRIMER_LEFT_6_TM=59.158
PRIMER_RIGHT_6_TM=61.141
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=862
PRIMER_PAIR_7_PENALTY=2.053590
PRIMER_LEFT_7_PENALTY=0.777580
PRIMER_RIGHT_7_PENALTY=1.276010
PRIMER_LEFT_7_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_7_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_7=671,20
PRIMER_RIGHT_7=1664,20
PRIMER_LEFT_7_TM=59.222
PRIMER_RIGHT_7_TM=58.724
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.8000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=994
PRIMER_PAIR_8_PENALTY=2.117772
PRIMER_LEFT_8_PENALTY=0.841762
PRIMER_RIGHT_8_PENALTY=1.276010
PRIMER_LEFT_8_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_8_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_8=826,20
PRIMER_RIGHT_8=1664,20
PRIMER_LEFT_8_TM=59.158
PRIMER_RIGHT_8_TM=58.724
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=839
PRIMER_PAIR_9_PENALTY=2.181722
PRIMER_LEFT_9_PENALTY=0.095771
PRIMER_RIGHT_9_PENALTY=2.085951
PRIMER_LEFT_9_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_9_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_9=419,20
PRIMER_RIGHT_9=1414,22
PRIMER_LEFT_9_TM=60.096
PRIMER_RIGHT_9_TM=59.914
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=996
PRIMER_PAIR_10_PENALTY=2.340266
PRIMER_LEFT_10_PENALTY=1.938224
PRIMER_RIGHT_10_PENALTY=0.402042
PRIMER_LEFT_10_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_10_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_10=825,20
PRIMER_RIGHT_10=1776,20
PRIMER_LEFT_10_TM=61.938
PRIMER_RIGHT_10_TM=59.598
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=8.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=952
PRIMER_PAIR_11_PENALTY=2.394411
PRIMER_LEFT_11_PENALTY=1.348580
PRIMER_RIGHT_11_PENALTY=1.045831
PRIMER_LEFT_11_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_11_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_11=665,20
PRIMER_RIGHT_11=1623,21
PRIMER_LEFT_11_TM=61.349
PRIMER_RIGHT_11_TM=59.954
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=959
PRIMER_PAIR_12_PENALTY=2.423693
PRIMER_LEFT_12_PENALTY=1.377862
PRIMER_RIGHT_12_PENALTY=1.045831
PRIMER_LEFT_12_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_12_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_12=672,21
PRIMER_RIGHT_12=1623,21
PRIMER_LEFT_12_TM=59.622
PRIMER_RIGHT_12_TM=59.954
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.5000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=952
PRIMER_PAIR_13_PENALTY=2.429008
PRIMER_LEFT_13_PENALTY=0.777580
PRIMER_RIGHT_13_PENALTY=1.651428
PRIMER_LEFT_13_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_13_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_13=671,20
PRIMER_RIGHT_13=1665,21
PRIMER_LEFT_13_TM=59.222
PRIMER_RIGHT_13_TM=60.651
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.8000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=995
PRIMER_PAIR_14_PENALTY=2.454032
PRIMER_LEFT_14_PENALTY=0.841762
PRIMER_RIGHT_14_PENALTY=1.612269
PRIMER_LEFT_14_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_14_SEQUENCE=GAGATGAGAATTGAGCACGG
PRIMER_LEFT_14=826,20
PRIMER_RIGHT_14=1667,20
PRIMER_LEFT_14_TM=59.158
PRIMER_RIGHT_14_TM=58.388
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=842
PRIMER_PAIR_15_PENALTY=2.474358
PRIMER_LEFT_15_PENALTY=0.388407
PRIMER_RIGHT_15_PENALTY=2.085951
PRIMER_LEFT_15_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_15_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_15=561,20
PRIMER_RIGHT_15=1414,22
PRIMER_LEFT_15_TM=59.612
PRIMER_RIGHT_15_TM=59.914
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.2000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=854
PRIMER_PAIR_16_PENALTY=2.493191
PRIMER_LEFT_16_PENALTY=0.841762
PRIMER_RIGHT_16_PENALTY=1.651428
PRIMER_LEFT_16_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_16_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_16=826,20
PRIMER_RIGHT_16=1665,21
PRIMER_LEFT_16_TM=59.158
PRIMER_RIGHT_16_TM=60.651
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=840
PRIMER_PAIR_17_PENALTY=2.544250
PRIMER_LEFT_17_PENALTY=0.841762
PRIMER_RIGHT_17_PENALTY=1.702488
PRIMER_LEFT_17_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_17_SEQUENCE=ATTGCCGATTGCTGAGATGA
PRIMER_LEFT_17=826,20
PRIMER_RIGHT_17=1680,20
PRIMER_LEFT_17_TM=59.158
PRIMER_RIGHT_17_TM=61.702
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=855
PRIMER_PAIR_18_PENALTY=2.571769
PRIMER_LEFT_18_PENALTY=0.841762
PRIMER_RIGHT_18_PENALTY=1.730006
PRIMER_LEFT_18_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_18_SEQUENCE=CATGTAGCTTGGGAGCGATG
PRIMER_LEFT_18=826,20
PRIMER_RIGHT_18=1750,20
PRIMER_LEFT_18_TM=59.158
PRIMER_RIGHT_18_TM=61.730
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=925
PRIMER_PAIR_19_PENALTY=2.605069
PRIMER_LEFT_19_PENALTY=1.938224
PRIMER_RIGHT_19_PENALTY=0.666846
PRIMER_LEFT_19_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_19_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_19=825,20
PRIMER_RIGHT_19=1730,20
PRIMER_LEFT_19_TM=61.938
PRIMER_RIGHT_19_TM=60.667
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=906
PRIMER_PAIR_20_PENALTY=2.615615
PRIMER_LEFT_20_PENALTY=0.841762
PRIMER_RIGHT_20_PENALTY=1.773852
PRIMER_LEFT_20_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_20_SEQUENCE=ATAGGTGACACGAACTCAGCC
PRIMER_LEFT_20=826,20
PRIMER_RIGHT_20=1786,21
PRIMER_LEFT_20_TM=59.158
PRIMER_RIGHT_20_TM=59.226
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=961
PRIMER_PAIR_21_PENALTY=2.624590
PRIMER_LEFT_21_PENALTY=1.348580
PRIMER_RIGHT_21_PENALTY=1.276010
PRIMER_LEFT_21_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_21_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_21=665,20
PRIMER_RIGHT_21=1664,20
PRIMER_LEFT_21_TM=61.349
PRIMER_RIGHT_21_TM=58.724
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=1000
PRIMER_PAIR_22_PENALTY=2.653872
PRIMER_LEFT_22_PENALTY=1.377862
PRIMER_RIGHT_22_PENALTY=1.276010
PRIMER_LEFT_22_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_22_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_22=672,21
PRIMER_RIGHT_22=1664,20
PRIMER_LEFT_22_TM=59.622
PRIMER_RIGHT_22_TM=58.724
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.5000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=993
PRIMER_PAIR_23_PENALTY=2.678904
PRIMER_LEFT_23_PENALTY=2.012058
PRIMER_RIGHT_23_PENALTY=0.666846
PRIMER_LEFT_23_SEQUENCE=CGAGGTCGAAAGAGAGGAAGA
PRIMER_RIGHT_23_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_23=826,21
PRIMER_RIGHT_23=1730,20
PRIMER_LEFT_23_TM=61.012
PRIMER_RIGHT_23_TM=60.667
PRIMER_LEFT_23_GC_PERCENT=52.381
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=905
PRIMER_PAIR_24_PENALTY=2.683451
PRIMER_LEFT_24_PENALTY=0.841762
PRIMER_RIGHT_24_PENALTY=1.841689
PRIMER_LEFT_24_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_24_SEQUENCE=CTCAGCCTCTCTTGGTGAAA
PRIMER_LEFT_24=826,20
PRIMER_RIGHT_24=1772,20
PRIMER_LEFT_24_TM=59.158
PRIMER_RIGHT_24_TM=58.158
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=8.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=947
PRIMER_PAIR_25_PENALTY=2.724911
PRIMER_LEFT_25_PENALTY=0.388407
PRIMER_RIGHT_25_PENALTY=2.336505
PRIMER_LEFT_25_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_25_SEQUENCE=CCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_25=561,20
PRIMER_RIGHT_25=1471,21
PRIMER_LEFT_25_TM=59.612
PRIMER_RIGHT_25_TM=58.663
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=10.2000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=911
PRIMER_PAIR_26_PENALTY=2.735536
PRIMER_LEFT_26_PENALTY=0.388407
PRIMER_RIGHT_26_PENALTY=2.347129
PRIMER_LEFT_26_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_26_SEQUENCE=CCATCCCTAAGTTCAGCTTGTT
PRIMER_LEFT_26=561,20
PRIMER_RIGHT_26=1475,22
PRIMER_LEFT_26_TM=59.612
PRIMER_RIGHT_26_TM=59.653
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.2000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=915
PRIMER_PAIR_27_PENALTY=2.765798
PRIMER_LEFT_27_PENALTY=0.841762
PRIMER_RIGHT_27_PENALTY=1.924036
PRIMER_LEFT_27_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_27_SEQUENCE=ACTCAGCCTCTCTTGGTGAAA
PRIMER_LEFT_27=826,20
PRIMER_RIGHT_27=1773,21
PRIMER_LEFT_27_TM=59.158
PRIMER_RIGHT_27_TM=59.076
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=8.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=948
PRIMER_PAIR_28_PENALTY=2.781195
PRIMER_LEFT_28_PENALTY=1.938224
PRIMER_RIGHT_28_PENALTY=0.842972
PRIMER_LEFT_28_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_28_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_28=825,20
PRIMER_RIGHT_28=1694,20
PRIMER_LEFT_28_TM=61.938
PRIMER_RIGHT_28_TM=60.843
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=870
PRIMER_PAIR_29_PENALTY=2.788857
PRIMER_LEFT_29_PENALTY=0.841762
PRIMER_RIGHT_29_PENALTY=1.947095
PRIMER_LEFT_29_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_29_SEQUENCE=CCAAACTGATTCATTGCCGA
PRIMER_LEFT_29=826,20
PRIMER_RIGHT_29=1692,20
PRIMER_LEFT_29_TM=59.158
PRIMER_RIGHT_29_TM=61.947
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=11.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=867
PRIMER_PAIR_30_PENALTY=2.810867
PRIMER_LEFT_30_PENALTY=1.938224
PRIMER_RIGHT_30_PENALTY=0.872644
PRIMER_LEFT_30_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_30_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_30=825,20
PRIMER_RIGHT_30=1783,20
PRIMER_LEFT_30_TM=61.938
PRIMER_RIGHT_30_TM=60.873
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=60.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=959
PRIMER_PAIR_31_PENALTY=2.812798
PRIMER_LEFT_31_PENALTY=0.388407
PRIMER_RIGHT_31_PENALTY=2.424392
PRIMER_LEFT_31_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_31_SEQUENCE=CCCTAAGTTCAGCTTGTTGCTC
PRIMER_LEFT_31=561,20
PRIMER_RIGHT_31=1471,22
PRIMER_LEFT_31_TM=59.612
PRIMER_RIGHT_31_TM=60.424
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=10.2000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=911
PRIMER_PAIR_32_PENALTY=2.812798
PRIMER_LEFT_32_PENALTY=0.388407
PRIMER_RIGHT_32_PENALTY=2.424392
PRIMER_LEFT_32_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_32_SEQUENCE=TCCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_32=561,20
PRIMER_RIGHT_32=1472,22
PRIMER_LEFT_32_TM=59.612
PRIMER_RIGHT_32_TM=60.424
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=10.2000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=912
PRIMER_PAIR_33_PENALTY=2.855030
PRIMER_LEFT_33_PENALTY=2.012058
PRIMER_RIGHT_33_PENALTY=0.842972
PRIMER_LEFT_33_SEQUENCE=CGAGGTCGAAAGAGAGGAAGA
PRIMER_RIGHT_33_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_33=826,21
PRIMER_RIGHT_33=1694,20
PRIMER_LEFT_33_TM=61.012
PRIMER_RIGHT_33_TM=60.843
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=869
PRIMER_PAIR_34_PENALTY=2.863531
PRIMER_LEFT_34_PENALTY=0.777580
PRIMER_RIGHT_34_PENALTY=2.085951
PRIMER_LEFT_34_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_34_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_34=671,20
PRIMER_RIGHT_34=1414,22
PRIMER_LEFT_34_TM=59.222
PRIMER_RIGHT_34_TM=59.914
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.8000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=744
PRIMER_PAIR_35_PENALTY=2.872108
PRIMER_LEFT_35_PENALTY=0.786156
PRIMER_RIGHT_35_PENALTY=2.085951
PRIMER_LEFT_35_SEQUENCE=CCATGTTCGTAGCTTCTCGG
PRIMER_RIGHT_35_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_35=432,20
PRIMER_RIGHT_35=1414,22
PRIMER_LEFT_35_TM=60.786
PRIMER_RIGHT_35_TM=59.914
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.9000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=983
PRIMER_PAIR_36_PENALTY=2.919343
PRIMER_LEFT_36_PENALTY=0.841762
PRIMER_RIGHT_36_PENALTY=2.077581
PRIMER_LEFT_36_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_36_SEQUENCE=GATTCATTGCCGATTGCTGA
PRIMER_LEFT_36=826,20
PRIMER_RIGHT_36=1685,20
PRIMER_LEFT_36_TM=59.158
PRIMER_RIGHT_36_TM=62.078
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=860
PRIMER_PAIR_37_PENALTY=2.927714
PRIMER_LEFT_37_PENALTY=0.841762
PRIMER_RIGHT_37_PENALTY=2.085951
PRIMER_LEFT_37_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_37_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_37=826,20
PRIMER_RIGHT_37=1414,22
PRIMER_LEFT_37_TM=59.158
PRIMER_RIGHT_37_TM=59.914
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=589
PRIMER_PAIR_38_PENALTY=2.947204
PRIMER_LEFT_38_PENALTY=1.901373
PRIMER_RIGHT_38_PENALTY=1.045831
PRIMER_LEFT_38_SEQUENCE=GCTGAGCTTGCTTTGCTTGA
PRIMER_RIGHT_38_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_38=667,20
PRIMER_RIGHT_38=1623,21
PRIMER_LEFT_38_TM=61.901
PRIMER_RIGHT_38_TM=59.954
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=957
PRIMER_PAIR_39_PENALTY=2.976022
PRIMER_LEFT_39_PENALTY=2.573980
PRIMER_RIGHT_39_PENALTY=0.402042
PRIMER_LEFT_39_SEQUENCE=ATAGACAATCTGCGGCGAGG
PRIMER_RIGHT_39_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_39=811,20
PRIMER_RIGHT_39=1776,20
PRIMER_LEFT_39_TM=62.574
PRIMER_RIGHT_39_TM=59.598
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.9000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=966
PRIMER_PAIR_40_PENALTY=2.976022
PRIMER_LEFT_40_PENALTY=2.573980
PRIMER_RIGHT_40_PENALTY=0.402042
PRIMER_LEFT_40_SEQUENCE=ATAGACAATCTGCGGCGAGG
PRIMER_RIGHT_40_SEQUENCE=ACGAACTCAGCCTCTCTTGG
PRIMER_LEFT_40=811,20
PRIMER_RIGHT_40=1777,20
PRIMER_LEFT_40_TM=62.574
PRIMER_RIGHT_40_TM=59.598
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=967
PRIMER_PAIR_41_PENALTY=2.984055
PRIMER_LEFT_41_PENALTY=1.938224
PRIMER_RIGHT_41_PENALTY=1.045831
PRIMER_LEFT_41_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_41_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_41=825,20
PRIMER_RIGHT_41=1623,21
PRIMER_LEFT_41_TM=61.938
PRIMER_RIGHT_41_TM=59.954
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=799
PRIMER_PAIR_42_PENALTY=3.005507
PRIMER_LEFT_42_PENALTY=0.095771
PRIMER_RIGHT_42_PENALTY=2.909736
PRIMER_LEFT_42_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_42_SEQUENCE=TGTTCAGCTCTGATGTTCCTCT
PRIMER_LEFT_42=419,20
PRIMER_RIGHT_42=1411,22
PRIMER_LEFT_42_TM=60.096
PRIMER_RIGHT_42_TM=59.090
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=993
PRIMER_PAIR_43_PENALTY=3.013865
PRIMER_LEFT_43_PENALTY=0.927913
PRIMER_RIGHT_43_PENALTY=2.085951
PRIMER_LEFT_43_SEQUENCE=TGTTCGTAGCTTCTCGGTTG
PRIMER_RIGHT_43_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_43=435,20
PRIMER_RIGHT_43=1414,22
PRIMER_LEFT_43_TM=59.072
PRIMER_RIGHT_43_TM=59.914
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=980
PRIMER_PAIR_44_PENALTY=3.015945
PRIMER_LEFT_44_PENALTY=0.841762
PRIMER_RIGHT_44_PENALTY=2.174183
PRIMER_LEFT_44_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_44_SEQUENCE=CTGCCCGTTTGTACTCATCT
PRIMER_LEFT_44=826,20
PRIMER_RIGHT_44=1729,20
PRIMER_LEFT_44_TM=59.158
PRIMER_RIGHT_44_TM=57.826
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=904
PRIMER_PAIR_45_PENALTY=3.020378
PRIMER_LEFT_45_PENALTY=0.934426
PRIMER_RIGHT_45_PENALTY=2.085951
PRIMER_LEFT_45_SEQUENCE=CGCCATGTTCGTAGCTTCTC
PRIMER_RIGHT_45_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_45=430,20
PRIMER_RIGHT_45=1414,22
PRIMER_LEFT_45_TM=60.934
PRIMER_RIGHT_45_TM=59.914
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=985
PRIMER_PAIR_46_PENALTY=3.029290
PRIMER_LEFT_46_PENALTY=1.377862
PRIMER_RIGHT_46_PENALTY=1.651428
PRIMER_LEFT_46_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_46_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_46=672,21
PRIMER_RIGHT_46=1665,21
PRIMER_LEFT_46_TM=59.622
PRIMER_RIGHT_46_TM=60.651
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.5000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=994
PRIMER_PAIR_47_PENALTY=3.056696
PRIMER_LEFT_47_PENALTY=1.938224
PRIMER_RIGHT_47_PENALTY=1.118473
PRIMER_LEFT_47_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_47_SEQUENCE=TAGGTGACACGAACTCAGCC
PRIMER_LEFT_47=825,20
PRIMER_RIGHT_47=1785,20
PRIMER_LEFT_47_TM=61.938
PRIMER_RIGHT_47_TM=58.882
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=961
PRIMER_PAIR_48_PENALTY=3.057135
PRIMER_LEFT_48_PENALTY=0.388407
PRIMER_RIGHT_48_PENALTY=2.668729
PRIMER_LEFT_48_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_48_SEQUENCE=CCATCCCTAAGTTCAGCTTGT
PRIMER_LEFT_48=561,20
PRIMER_RIGHT_48=1475,21
PRIMER_LEFT_48_TM=59.612
PRIMER_RIGHT_48_TM=58.331
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=10.2000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=915
PRIMER_PAIR_49_PENALTY=3.057890
PRIMER_LEFT_49_PENALTY=2.012058
PRIMER_RIGHT_49_PENALTY=1.045831
PRIMER_LEFT_49_SEQUENCE=CGAGGTCGAAAGAGAGGAAGA
PRIMER_RIGHT_49_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_49=826,21
PRIMER_RIGHT_49=1623,21
PRIMER_LEFT_49_TM=61.012
PRIMER_RIGHT_49_TM=59.954
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=798
=
SEQUENCE_ID=MAGIC.105_3_894869_895187_col-0_ler-0_42030_5716_TRUE
SEQUENCE_TARGET=950,418
SEQUENCE_TEMPLATE=CTACTACTTCGCCAATTGCGTCCNGTGGAATTTATTAAATATCTTAACATTACTTNGTATATTTCTCCACATCCAATNAACGTCTTATCGTGACCTAATTTGTATTTTCTTCAGCGAAATAGATTAATTTTGGCGTCATGCGACAGAGGCGCCTAAAAAGATATTTTTTGTCACTTAGGTGAAAAAAATCAAAATGTTTTTCTTTGTCACTAAGTTTTGAACACTTACAAAAATGAGGAAAATCATTGTAGGGGTAGTTTCGTAAAATGGTTTGTAAGGAAATTAAATAATTTACAGATTGTAAAATGAATATATGTTTGACCATGTCAAANTTTTTTTATTACATATTGTGGCATATTAAGTAATTTTAAATTTCGAATAACATTATTATGAAGAAGAAAAAAGGAAAAAGCAATAAAGAAGGACGATCCAAACAGAAGGTCAAGAAAAAACCGTGCAATTATTTATTACATATGACGTTAAGTAAACATGGCTTACACAGTGGGTAGCCATTCGATTTTTATTTTGTTCGTGCAAAATAATATTATACCATATTAATTTGACTGCTAAGATTTAATTGTGCGCTACGAGTAATTTTTTTCATTTTTTTGTAAATTTATTCTCTATTATCTTGTTTGTTTTATTCATATGAGAACAATGATTTTTGGTTTATATCTAGCATGTTTTTGGTATTAGAAAATCTTTGGTCAAGACTCTAAGAACGANTAAAAAATAATTTTCTGATCATAGTTGATCAGAGAAGTTTGTAAAACAATAATAAAAATGATGAGAAANAGATATTTAGTTTNTCAAGGGTACCTTGGTAATTGAGTCTAGATTTCTGCTCAGGCATCTTTTTCTTTTTTGGCCAAAACTCAAGCATCTTAATATTTCAAAAACTAAAAGAACTGNATACGTCATTTNNNAAAATAAATTAATTAAGTCATTTCTTCTTTACACAGTCTAGCCACTTATAAGAGTGGTAAACTTAACTAATACANCTAAATGGAACCAAACATNTCNTGGTGTATTGGATCTTGGAGTTTATTAATTACCACACAAGTGANTGTNTTATACACTGTTATATAATCCATTGCTCATAAATACATGGATCTTTCCCGATTAGTAATTATGTCANACCGTAACGATTACGATCGAGAGTAGAGAGTTACACATATTCATGCATGTTATTTAATGTAAATGTGAAATTCATTTTTTTAAAGTACATNTATGTGAATTAGTGTATATTCAGTGTACAAATGATNCATGGGAATGGGGTGTATATTCAGTGTACAATGATGTATGATCAAGATANTTTNTNNNNTTTCTTTNTTTCTNAGGGAGAATGTATATATTAAACTTTAAATGGAGAATAAAATAGTCCGTACATAGGTGAGTGTGCGAATACGTATGCAAAAAGTAGAGTGTAGAAAGACACAGTAGGAGACAACTTGCTTCTTCAGATGCCCTCTGTTTTTGCCTNNNGTCTCTGTATAATTGTATAGCTGTTTTGGTNTTTTTTCGTTTTNATATTTTTAATATTANAATACTCATTAACTACGTAGAATAAGTAATTGTTTCTTTTTNGTTTCTCNTNTAGCTGTNTAAGTAAATGTATTGNTGTTTATNTGTTTGGTGTTGCTTCAANAAATGATAGCTATACGTAAATATTCATAGTTATGGGCTCGGCTATCTGTATCNGNGTCANTNTATATGTTATTAATACTTCTATATNAANGTTAATATTATATAACTGAATTCATTCTACTTTACAAATTTTCTGAAACTTTATNNNTNTNTNGTTAAATAATTTTNTTTGCATATTTTTAGATANAAATAAATCNAAATCAAAACTACAATCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNATATTTTAGGNAAAAAGGNGANGTNTAATAATTGNCAGAAATTTATTACTATTTAAATANAANTAGTTCAATGAAACATANAATTAAAATGGNNNNNNNTAACCAATATAATTACTTCAACGAAATAAATTGAAATAGGTAAAAATGAGGAGATTNATTTATNTTANAATATTAGNATNNANNNNNNNNNNNNNNNNTNTATATNGAATAGCTCTNGAAGTGAGATNTGTGTGTAGATAATAGATCTGTCAGGAAGTCATACGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGTTGTATCAAATCNCTGAGAGAAACCACTTTTAGATATCNAGAAACTCTACTCCTTAATTCANTTAAATAAGTTNNGCGAAANTTTTCTAAAN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.015391
PRIMER_LEFT_0_PENALTY=0.589306
PRIMER_RIGHT_0_PENALTY=0.426086
PRIMER_LEFT_0_SEQUENCE=TGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_0_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_0=502,20
PRIMER_RIGHT_0=1469,20
PRIMER_LEFT_0_TM=59.411
PRIMER_RIGHT_0_TM=59.574
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=968
PRIMER_PAIR_1_PENALTY=1.154912
PRIMER_LEFT_1_PENALTY=0.589306
PRIMER_RIGHT_1_PENALTY=0.565606
PRIMER_LEFT_1_SEQUENCE=TGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_1_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_1=502,20
PRIMER_RIGHT_1=1468,20
PRIMER_LEFT_1_TM=59.411
PRIMER_RIGHT_1_TM=59.434
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=967
PRIMER_PAIR_2_PENALTY=1.519975
PRIMER_LEFT_2_PENALTY=1.093890
PRIMER_RIGHT_2_PENALTY=0.426086
PRIMER_LEFT_2_SEQUENCE=GTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_2_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_2=501,20
PRIMER_RIGHT_2=1469,20
PRIMER_LEFT_2_TM=58.906
PRIMER_RIGHT_2_TM=59.574
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=969
PRIMER_PAIR_3_PENALTY=1.577189
PRIMER_LEFT_3_PENALTY=1.151103
PRIMER_RIGHT_3_PENALTY=0.426086
PRIMER_LEFT_3_SEQUENCE=AGTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_3_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_3=500,21
PRIMER_RIGHT_3=1469,20
PRIMER_LEFT_3_TM=59.849
PRIMER_RIGHT_3_TM=59.574
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=970
PRIMER_PAIR_4_PENALTY=1.623585
PRIMER_LEFT_4_PENALTY=1.197499
PRIMER_RIGHT_4_PENALTY=0.426086
PRIMER_LEFT_4_SEQUENCE=GTGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_4_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_4=501,21
PRIMER_RIGHT_4=1469,20
PRIMER_LEFT_4_TM=60.197
PRIMER_RIGHT_4_TM=59.574
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=969
PRIMER_PAIR_5_PENALTY=1.659496
PRIMER_LEFT_5_PENALTY=1.093890
PRIMER_RIGHT_5_PENALTY=0.565606
PRIMER_LEFT_5_SEQUENCE=GTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_5_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_5=501,20
PRIMER_RIGHT_5=1468,20
PRIMER_LEFT_5_TM=58.906
PRIMER_RIGHT_5_TM=59.434
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=968
PRIMER_PAIR_6_PENALTY=1.716709
PRIMER_LEFT_6_PENALTY=1.151103
PRIMER_RIGHT_6_PENALTY=0.565606
PRIMER_LEFT_6_SEQUENCE=AGTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_6_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_6=500,21
PRIMER_RIGHT_6=1468,20
PRIMER_LEFT_6_TM=59.849
PRIMER_RIGHT_6_TM=59.434
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.2000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=969
PRIMER_PAIR_7_PENALTY=1.726255
PRIMER_LEFT_7_PENALTY=1.416967
PRIMER_RIGHT_7_PENALTY=0.309288
PRIMER_LEFT_7_SEQUENCE=TTCTGCTCAGGCATCTTTTTC
PRIMER_RIGHT_7_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_7=839,21
PRIMER_RIGHT_7=1695,20
PRIMER_LEFT_7_TM=59.583
PRIMER_RIGHT_7_TM=59.691
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=5.6000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=857
PRIMER_PAIR_8_PENALTY=1.763105
PRIMER_LEFT_8_PENALTY=1.197499
PRIMER_RIGHT_8_PENALTY=0.565606
PRIMER_LEFT_8_SEQUENCE=GTGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_8_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_8=501,21
PRIMER_RIGHT_8=1468,20
PRIMER_LEFT_8_TM=60.197
PRIMER_RIGHT_8_TM=59.434
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=968
PRIMER_PAIR_9_PENALTY=1.982573
PRIMER_LEFT_9_PENALTY=1.416967
PRIMER_RIGHT_9_PENALTY=0.565606
PRIMER_LEFT_9_SEQUENCE=TTCTGCTCAGGCATCTTTTTC
PRIMER_RIGHT_9_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_9=839,21
PRIMER_RIGHT_9=1468,20
PRIMER_LEFT_9_TM=59.583
PRIMER_RIGHT_9_TM=59.434
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=630
PRIMER_PAIR_10_PENALTY=1.986066
PRIMER_LEFT_10_PENALTY=0.589306
PRIMER_RIGHT_10_PENALTY=1.396760
PRIMER_LEFT_10_SEQUENCE=TGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_10_SEQUENCE=AGGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_10=502,20
PRIMER_RIGHT_10=1469,21
PRIMER_LEFT_10_TM=59.411
PRIMER_RIGHT_10_TM=60.397
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=968
PRIMER_PAIR_11_PENALTY=2.100825
PRIMER_LEFT_11_PENALTY=1.791537
PRIMER_RIGHT_11_PENALTY=0.309288
PRIMER_LEFT_11_SEQUENCE=TCTGCTCAGGCATCTTTTTCT
PRIMER_RIGHT_11_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_11=840,21
PRIMER_RIGHT_11=1695,20
PRIMER_LEFT_11_TM=59.208
PRIMER_RIGHT_11_TM=59.691
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=5.6000
PRIMER_PAIR_11_COMPL_ANY=7.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=856
PRIMER_PAIR_12_PENALTY=2.100825
PRIMER_LEFT_12_PENALTY=1.791537
PRIMER_RIGHT_12_PENALTY=0.309288
PRIMER_LEFT_12_SEQUENCE=TCTGCTCAGGCATCTTTTTCT
PRIMER_RIGHT_12_SEQUENCE=ACAGATAGCCGAGCCCATAA
PRIMER_LEFT_12=840,21
PRIMER_RIGHT_12=1696,20
PRIMER_LEFT_12_TM=59.208
PRIMER_RIGHT_12_TM=59.691
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.2000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=857
PRIMER_PAIR_13_PENALTY=2.136226
PRIMER_LEFT_13_PENALTY=1.826937
PRIMER_RIGHT_13_PENALTY=0.309288
PRIMER_LEFT_13_SEQUENCE=TCTGCTCAGGCATCTTTTTC
PRIMER_RIGHT_13_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_13=840,20
PRIMER_RIGHT_13=1695,20
PRIMER_LEFT_13_TM=58.173
PRIMER_RIGHT_13_TM=59.691
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=5.6000
PRIMER_PAIR_13_COMPL_ANY=7.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=856
PRIMER_PAIR_14_PENALTY=2.330483
PRIMER_LEFT_14_PENALTY=2.021195
PRIMER_RIGHT_14_PENALTY=0.309288
PRIMER_LEFT_14_SEQUENCE=CTGCTCAGGCATCTTTTTCTTT
PRIMER_RIGHT_14_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_14=841,22
PRIMER_RIGHT_14=1695,20
PRIMER_LEFT_14_TM=60.021
PRIMER_RIGHT_14_TM=59.691
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=5.6000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=855
PRIMER_PAIR_15_PENALTY=2.330483
PRIMER_LEFT_15_PENALTY=2.021195
PRIMER_RIGHT_15_PENALTY=0.309288
PRIMER_LEFT_15_SEQUENCE=CTGCTCAGGCATCTTTTTCTTT
PRIMER_RIGHT_15_SEQUENCE=ACAGATAGCCGAGCCCATAA
PRIMER_LEFT_15=841,22
PRIMER_RIGHT_15=1696,20
PRIMER_LEFT_15_TM=60.021
PRIMER_RIGHT_15_TM=59.691
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.2000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=856
PRIMER_PAIR_16_PENALTY=2.345699
PRIMER_LEFT_16_PENALTY=0.589306
PRIMER_RIGHT_16_PENALTY=1.756394
PRIMER_LEFT_16_SEQUENCE=TGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_16_SEQUENCE=ACGTATTCGCACACTCACCT
PRIMER_LEFT_16=502,20
PRIMER_RIGHT_16=1409,20
PRIMER_LEFT_16_TM=59.411
PRIMER_RIGHT_16_TM=58.244
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=908
PRIMER_PAIR_17_PENALTY=2.357143
PRIMER_LEFT_17_PENALTY=1.791537
PRIMER_RIGHT_17_PENALTY=0.565606
PRIMER_LEFT_17_SEQUENCE=TCTGCTCAGGCATCTTTTTCT
PRIMER_RIGHT_17_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_17=840,21
PRIMER_RIGHT_17=1468,20
PRIMER_LEFT_17_TM=59.208
PRIMER_RIGHT_17_TM=59.434
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=629
PRIMER_PAIR_18_PENALTY=2.392543
PRIMER_LEFT_18_PENALTY=1.826937
PRIMER_RIGHT_18_PENALTY=0.565606
PRIMER_LEFT_18_SEQUENCE=TCTGCTCAGGCATCTTTTTC
PRIMER_RIGHT_18_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_18=840,20
PRIMER_RIGHT_18=1468,20
PRIMER_LEFT_18_TM=58.173
PRIMER_RIGHT_18_TM=59.434
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=629
PRIMER_PAIR_19_PENALTY=2.490650
PRIMER_LEFT_19_PENALTY=1.093890
PRIMER_RIGHT_19_PENALTY=1.396760
PRIMER_LEFT_19_SEQUENCE=GTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_19_SEQUENCE=AGGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_19=501,20
PRIMER_RIGHT_19=1469,21
PRIMER_LEFT_19_TM=58.906
PRIMER_RIGHT_19_TM=60.397
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=969
PRIMER_PAIR_20_PENALTY=2.547864
PRIMER_LEFT_20_PENALTY=1.151103
PRIMER_RIGHT_20_PENALTY=1.396760
PRIMER_LEFT_20_SEQUENCE=AGTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_20_SEQUENCE=AGGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_20=500,21
PRIMER_RIGHT_20=1469,21
PRIMER_LEFT_20_TM=59.849
PRIMER_RIGHT_20_TM=60.397
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=970
PRIMER_PAIR_21_PENALTY=2.563636
PRIMER_LEFT_21_PENALTY=2.137550
PRIMER_RIGHT_21_PENALTY=0.426086
PRIMER_LEFT_21_SEQUENCE=GATTTCTGCTCAGGCATCTTTT
PRIMER_RIGHT_21_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_21=836,22
PRIMER_RIGHT_21=1469,20
PRIMER_LEFT_21_TM=59.862
PRIMER_RIGHT_21_TM=59.574
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=634
PRIMER_PAIR_22_PENALTY=2.577709
PRIMER_LEFT_22_PENALTY=2.268421
PRIMER_RIGHT_22_PENALTY=0.309288
PRIMER_LEFT_22_SEQUENCE=CTGCTCAGGCATCTTTTTCTT
PRIMER_RIGHT_22_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_22=841,21
PRIMER_RIGHT_22=1695,20
PRIMER_LEFT_22_TM=58.732
PRIMER_RIGHT_22_TM=59.691
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=5.6000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=855
PRIMER_PAIR_23_PENALTY=2.577709
PRIMER_LEFT_23_PENALTY=2.268421
PRIMER_RIGHT_23_PENALTY=0.309288
PRIMER_LEFT_23_SEQUENCE=CTGCTCAGGCATCTTTTTCTT
PRIMER_RIGHT_23_SEQUENCE=ACAGATAGCCGAGCCCATAA
PRIMER_LEFT_23=841,21
PRIMER_RIGHT_23=1696,20
PRIMER_LEFT_23_TM=58.732
PRIMER_RIGHT_23_TM=59.691
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.2000
PRIMER_PAIR_23_COMPL_ANY=7.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=856
PRIMER_PAIR_24_PENALTY=2.586801
PRIMER_LEFT_24_PENALTY=2.021195
PRIMER_RIGHT_24_PENALTY=0.565606
PRIMER_LEFT_24_SEQUENCE=CTGCTCAGGCATCTTTTTCTTT
PRIMER_RIGHT_24_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_24=841,22
PRIMER_RIGHT_24=1468,20
PRIMER_LEFT_24_TM=60.021
PRIMER_RIGHT_24_TM=59.434
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=628
PRIMER_PAIR_25_PENALTY=2.594259
PRIMER_LEFT_25_PENALTY=1.197499
PRIMER_RIGHT_25_PENALTY=1.396760
PRIMER_LEFT_25_SEQUENCE=GTGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_25_SEQUENCE=AGGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_25=501,21
PRIMER_RIGHT_25=1469,21
PRIMER_LEFT_25_TM=60.197
PRIMER_RIGHT_25_TM=60.397
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=969
PRIMER_PAIR_26_PENALTY=2.610911
PRIMER_LEFT_26_PENALTY=2.301623
PRIMER_RIGHT_26_PENALTY=0.309288
PRIMER_LEFT_26_SEQUENCE=TGCTCAGGCATCTTTTTCTT
PRIMER_RIGHT_26_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_26=842,20
PRIMER_RIGHT_26=1695,20
PRIMER_LEFT_26_TM=57.698
PRIMER_RIGHT_26_TM=59.691
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=5.6000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=854
PRIMER_PAIR_27_PENALTY=2.610911
PRIMER_LEFT_27_PENALTY=2.301623
PRIMER_RIGHT_27_PENALTY=0.309288
PRIMER_LEFT_27_SEQUENCE=TGCTCAGGCATCTTTTTCTT
PRIMER_RIGHT_27_SEQUENCE=ACAGATAGCCGAGCCCATAA
PRIMER_LEFT_27=842,20
PRIMER_RIGHT_27=1696,20
PRIMER_LEFT_27_TM=57.698
PRIMER_RIGHT_27_TM=59.691
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.2000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=855
PRIMER_PAIR_28_PENALTY=2.610911
PRIMER_LEFT_28_PENALTY=2.301623
PRIMER_RIGHT_28_PENALTY=0.309288
PRIMER_LEFT_28_SEQUENCE=TTCTGCTCAGGCATCTTTTT
PRIMER_RIGHT_28_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_28=839,20
PRIMER_RIGHT_28=1695,20
PRIMER_LEFT_28_TM=57.698
PRIMER_RIGHT_28_TM=59.691
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=5.6000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=857
PRIMER_PAIR_29_PENALTY=2.619373
PRIMER_LEFT_29_PENALTY=0.862979
PRIMER_RIGHT_29_PENALTY=1.756394
PRIMER_LEFT_29_SEQUENCE=AGGACGATCCAAACAGAAGG
PRIMER_RIGHT_29_SEQUENCE=ACGTATTCGCACACTCACCT
PRIMER_LEFT_29=421,20
PRIMER_RIGHT_29=1409,20
PRIMER_LEFT_29_TM=59.137
PRIMER_RIGHT_29_TM=58.244
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=989
PRIMER_PAIR_30_PENALTY=2.703157
PRIMER_LEFT_30_PENALTY=2.137550
PRIMER_RIGHT_30_PENALTY=0.565606
PRIMER_LEFT_30_SEQUENCE=GATTTCTGCTCAGGCATCTTTT
PRIMER_RIGHT_30_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_30=836,22
PRIMER_RIGHT_30=1468,20
PRIMER_LEFT_30_TM=59.862
PRIMER_RIGHT_30_TM=59.434
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=633
PRIMER_PAIR_31_PENALTY=2.727709
PRIMER_LEFT_31_PENALTY=2.301623
PRIMER_RIGHT_31_PENALTY=0.426086
PRIMER_LEFT_31_SEQUENCE=TTCTGCTCAGGCATCTTTTT
PRIMER_RIGHT_31_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_31=839,20
PRIMER_RIGHT_31=1469,20
PRIMER_LEFT_31_TM=57.698
PRIMER_RIGHT_31_TM=59.574
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=631
PRIMER_PAIR_32_PENALTY=2.727709
PRIMER_LEFT_32_PENALTY=2.301623
PRIMER_RIGHT_32_PENALTY=0.426086
PRIMER_LEFT_32_SEQUENCE=TTTCTGCTCAGGCATCTTTT
PRIMER_RIGHT_32_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_32=838,20
PRIMER_RIGHT_32=1469,20
PRIMER_LEFT_32_TM=57.698
PRIMER_RIGHT_32_TM=59.574
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=632
PRIMER_PAIR_33_PENALTY=2.811849
PRIMER_LEFT_33_PENALTY=2.502561
PRIMER_RIGHT_33_PENALTY=0.309288
PRIMER_LEFT_33_SEQUENCE=TCTGCTCAGGCATCTTTTTCTT
PRIMER_RIGHT_33_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_33=840,22
PRIMER_RIGHT_33=1695,20
PRIMER_LEFT_33_TM=60.503
PRIMER_RIGHT_33_TM=59.691
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=5.6000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=856
PRIMER_PAIR_34_PENALTY=2.811849
PRIMER_LEFT_34_PENALTY=2.502561
PRIMER_RIGHT_34_PENALTY=0.309288
PRIMER_LEFT_34_SEQUENCE=TCTGCTCAGGCATCTTTTTCTT
PRIMER_RIGHT_34_SEQUENCE=ACAGATAGCCGAGCCCATAA
PRIMER_LEFT_34=840,22
PRIMER_RIGHT_34=1696,20
PRIMER_LEFT_34_TM=60.503
PRIMER_RIGHT_34_TM=59.691
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.2000
PRIMER_PAIR_34_COMPL_ANY=7.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=857
PRIMER_PAIR_35_PENALTY=2.811849
PRIMER_LEFT_35_PENALTY=2.502561
PRIMER_RIGHT_35_PENALTY=0.309288
PRIMER_LEFT_35_SEQUENCE=TTCTGCTCAGGCATCTTTTTCT
PRIMER_RIGHT_35_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_35=839,22
PRIMER_RIGHT_35=1695,20
PRIMER_LEFT_35_TM=60.503
PRIMER_RIGHT_35_TM=59.691
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=5.6000
PRIMER_PAIR_35_COMPL_ANY=7.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=857
PRIMER_PAIR_36_PENALTY=2.813727
PRIMER_LEFT_36_PENALTY=1.416967
PRIMER_RIGHT_36_PENALTY=1.396760
PRIMER_LEFT_36_SEQUENCE=TTCTGCTCAGGCATCTTTTTC
PRIMER_RIGHT_36_SEQUENCE=AGGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_36=839,21
PRIMER_RIGHT_36=1469,21
PRIMER_LEFT_36_TM=59.583
PRIMER_RIGHT_36_TM=60.397
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=631
PRIMER_PAIR_37_PENALTY=2.850284
PRIMER_LEFT_37_PENALTY=1.093890
PRIMER_RIGHT_37_PENALTY=1.756394
PRIMER_LEFT_37_SEQUENCE=GTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_37_SEQUENCE=ACGTATTCGCACACTCACCT
PRIMER_LEFT_37=501,20
PRIMER_RIGHT_37=1409,20
PRIMER_LEFT_37_TM=58.906
PRIMER_RIGHT_37_TM=58.244
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.2000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=909
PRIMER_PAIR_38_PENALTY=2.867229
PRIMER_LEFT_38_PENALTY=2.301623
PRIMER_RIGHT_38_PENALTY=0.565606
PRIMER_LEFT_38_SEQUENCE=TTCTGCTCAGGCATCTTTTT
PRIMER_RIGHT_38_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_38=839,20
PRIMER_RIGHT_38=1468,20
PRIMER_LEFT_38_TM=57.698
PRIMER_RIGHT_38_TM=59.434
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=630
PRIMER_PAIR_39_PENALTY=2.867229
PRIMER_LEFT_39_PENALTY=2.301623
PRIMER_RIGHT_39_PENALTY=0.565606
PRIMER_LEFT_39_SEQUENCE=TTTCTGCTCAGGCATCTTTT
PRIMER_RIGHT_39_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_39=838,20
PRIMER_RIGHT_39=1468,20
PRIMER_LEFT_39_TM=57.698
PRIMER_RIGHT_39_TM=59.434
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=631
PRIMER_PAIR_40_PENALTY=2.883147
PRIMER_LEFT_40_PENALTY=2.457061
PRIMER_RIGHT_40_PENALTY=0.426086
PRIMER_LEFT_40_SEQUENCE=GATTTCTGCTCAGGCATCTTT
PRIMER_RIGHT_40_SEQUENCE=AGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_40=836,21
PRIMER_RIGHT_40=1469,20
PRIMER_LEFT_40_TM=58.543
PRIMER_RIGHT_40_TM=59.574
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=634
PRIMER_PAIR_41_PENALTY=2.902368
PRIMER_LEFT_41_PENALTY=1.416967
PRIMER_RIGHT_41_PENALTY=1.485401
PRIMER_LEFT_41_SEQUENCE=TTCTGCTCAGGCATCTTTTTC
PRIMER_RIGHT_41_SEQUENCE=ACAGATAGCCGAGCCCATAAC
PRIMER_LEFT_41=839,21
PRIMER_RIGHT_41=1696,21
PRIMER_LEFT_41_TM=59.583
PRIMER_RIGHT_41_TM=60.485
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=5.6000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=858
PRIMER_PAIR_42_PENALTY=2.907497
PRIMER_LEFT_42_PENALTY=1.151103
PRIMER_RIGHT_42_PENALTY=1.756394
PRIMER_LEFT_42_SEQUENCE=AGTGGGTAGCCATTCGATTTT
PRIMER_RIGHT_42_SEQUENCE=ACGTATTCGCACACTCACCT
PRIMER_LEFT_42=500,21
PRIMER_RIGHT_42=1409,20
PRIMER_LEFT_42_TM=59.849
PRIMER_RIGHT_42_TM=58.244
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.2000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=910
PRIMER_PAIR_43_PENALTY=2.953893
PRIMER_LEFT_43_PENALTY=1.197499
PRIMER_RIGHT_43_PENALTY=1.756394
PRIMER_LEFT_43_SEQUENCE=GTGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_43_SEQUENCE=ACGTATTCGCACACTCACCT
PRIMER_LEFT_43=501,21
PRIMER_RIGHT_43=1409,20
PRIMER_LEFT_43_TM=60.197
PRIMER_RIGHT_43_TM=58.244
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=909
PRIMER_PAIR_44_PENALTY=2.993660
PRIMER_LEFT_44_PENALTY=0.589306
PRIMER_RIGHT_44_PENALTY=2.404354
PRIMER_LEFT_44_SEQUENCE=TGGGTAGCCATTCGATTTTT
PRIMER_RIGHT_44_SEQUENCE=GAGGGCATCTGAAGAAGCAAG
PRIMER_LEFT_44=502,20
PRIMER_RIGHT_44=1470,21
PRIMER_LEFT_44_TM=59.411
PRIMER_RIGHT_44_TM=61.404
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=969
PRIMER_PAIR_45_PENALTY=3.011263
PRIMER_LEFT_45_PENALTY=2.701974
PRIMER_RIGHT_45_PENALTY=0.309288
PRIMER_LEFT_45_SEQUENCE=CTGCTCAGGCATCTTTTTCT
PRIMER_RIGHT_45_SEQUENCE=CAGATAGCCGAGCCCATAAC
PRIMER_LEFT_45=841,20
PRIMER_RIGHT_45=1695,20
PRIMER_LEFT_45_TM=57.298
PRIMER_RIGHT_45_TM=59.691
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=5.6000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=855
PRIMER_PAIR_46_PENALTY=3.011263
PRIMER_LEFT_46_PENALTY=2.701974
PRIMER_RIGHT_46_PENALTY=0.309288
PRIMER_LEFT_46_SEQUENCE=CTGCTCAGGCATCTTTTTCT
PRIMER_RIGHT_46_SEQUENCE=ACAGATAGCCGAGCCCATAA
PRIMER_LEFT_46=841,20
PRIMER_RIGHT_46=1696,20
PRIMER_LEFT_46_TM=57.298
PRIMER_RIGHT_46_TM=59.691
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.2000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=856
PRIMER_PAIR_47_PENALTY=3.022668
PRIMER_LEFT_47_PENALTY=2.457061
PRIMER_RIGHT_47_PENALTY=0.565606
PRIMER_LEFT_47_SEQUENCE=GATTTCTGCTCAGGCATCTTT
PRIMER_RIGHT_47_SEQUENCE=GGGCATCTGAAGAAGCAAGT
PRIMER_LEFT_47=836,21
PRIMER_RIGHT_47=1468,20
PRIMER_LEFT_47_TM=58.543
PRIMER_RIGHT_47_TM=59.434
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=633
PRIMER_PAIR_48_PENALTY=3.023789
PRIMER_LEFT_48_PENALTY=1.416967
PRIMER_RIGHT_48_PENALTY=1.606821
PRIMER_LEFT_48_SEQUENCE=TTCTGCTCAGGCATCTTTTTC
PRIMER_RIGHT_48_SEQUENCE=CAGATAGCCGAGCCCATAACT
PRIMER_LEFT_48=839,21
PRIMER_RIGHT_48=1695,21
PRIMER_LEFT_48_TM=59.583
PRIMER_RIGHT_48_TM=60.607
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=5.7000
PRIMER_PAIR_48_COMPL_ANY=7.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=857
PRIMER_PAIR_49_PENALTY=3.036609
PRIMER_LEFT_49_PENALTY=1.280215
PRIMER_RIGHT_49_PENALTY=1.756394
PRIMER_LEFT_49_SEQUENCE=CGATCCAAACAGAAGGTCAAG
PRIMER_RIGHT_49_SEQUENCE=ACGTATTCGCACACTCACCT
PRIMER_LEFT_49=425,21
PRIMER_RIGHT_49=1409,20
PRIMER_LEFT_49_TM=59.720
PRIMER_RIGHT_49_TM=58.244
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=985
=
SEQUENCE_ID=MAGIC.426_5_12782600_12783205_oy-0_kn-0_19319_135408_TRUE
SEQUENCE_TARGET=950,705
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNCTATANTAAGTTTNCACAAAAATNNGTGTGTCTNTNATNGNCNNTGGCCCTANTATTTATNCCTAATAATTACACTTAANTCTCATTTANACTTAATTATTNTNTCTTAAATGTATCAATTTTGGTNTTCGAATTTACTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNNNNNTTNNNCTNGCGGTACGANCNCTTCGCCCGCATTCTTNTCTTCNACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGCAANNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAATGCNNNTNAATNGACAAAGATTGCGCNAGATGATTTTCTGATTTC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_15536395_15536737_ler-0_edi-0_25030_30897_TRUE
SEQUENCE_TARGET=950,442
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTGATAAGNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_18977057_18977127_oy-0_wil-2_2472784_5716_TRUE
SEQUENCE_TARGET=950,170
SEQUENCE_TEMPLATE=NTTTAGAGCTTTAAAAGTCATATGTCGTTTTGTTTCGCTGCTTTCTCTGAACCTCCTTTAATGTTGTATCTANGGTAAAACATATAATCTCTTTAACCTTGGAGNGAATATGAAACCGTTCGAAATTTTANTACATGACACTAAGACATGGCCACATGGGGACATTCGACATCCATTTGATCTACNTNATTATTTAAGTTTGTTAATATNTCAANGTCATGATCCCGTTGGTTCCNTTTCGATAGTTATTGAAAATGCGATAGTTATTTTTTAAACTCCATTTTGGTAGACTGATAGATAGCATTGTCATTNATCAATAATAATAATTTGGTATTCCTCACTATATATTTTGTGCTAGGGTTAATAATTTGTTTTANTGTCGTTATCGCACCATANCACTAGTATCTAAGTTCAAACTTGCATAATGTATTCAGTTNATAATACTTTTTATATCAATACTTCAAAGGANAACACAATTATTCAAAACGAGGCATATTTTACAAGCATGGAAGCTACATGCATGANTATGCATAACNNNNNNNNNNNNNNNNNNNTAGGATCCTCTTTGTAAGAGATACAAATTTTNGAATTATGTCTTACTTATATANNAATTTGTTATAAACTCACTTTGGATAATTATTGCCAAATTGCTATCCTTTAANCATNCNTTTATTATTAAAAGGNAAGTACAAATTNAAATTTTGTCTAATTNNNCCTCAATTTTGATTTCAATTTTGGATTTTGTTCTTTGCTATTCAAAATCATTAAGNGTAATTTGGTCTTAACAATNTCCTAAACTTTATATGATNTGATNCAATCTAAACCGTTCAATAAAATTAAATCTCTTCAATCTAAACCNTTCAACNAAAATAAGGAATNTATGTCGTTTTTGAAATCAAAATATGATCACATTCCATNNCTNAAAACACTATATTNAATCACGAAATCAATAATCAATAATATCAAAATNATATATTANATTCTTCACTAATCACNAAAANTTTATANTTATAAGTTTANNATATCAAATTTATTCAAATATTTAGTATNAAAATNAAATAAANCGATTGNCCNCGGTTTACCGCGGGTTAAAACNTANTAATACTAATTAGTATACCTAATTGATGTAGTTGNAACTTTTAAAAGAGNGAAACCGAAGAGAGGCGTNGGTGGAGTCACGTGATGAGCATTTCTCACCCGATTCAATTTCAACTATTTTAGCTTTTACATAAATGTGGTCCATTGTCTAATTTCTTCCATCCCCACTAAAAGTTTCACGAAATAAGNTTCATTCTTAACTACTATCACATAAAATTGGTAGATTTTTAAAGCTCCGACTACAAAGATAATATATAGATTGGTTCCGTACCGTTATTCTCGAGTTGTGNGCCCAAANTTTTTGGGAGATCCTTGAGCCAACAACCACTTGTTTACATGTGCTTNTGCCAGTACTTTCTCTCATCTTGACATTATTTCTTTGGTTCTCTTTACGTTTGGTGACTTTCNTCTATTAAAATTAACACAAGACATATATTGCTAGTTAGATATAATATTCATNACATGTGTATTTTGAAAACATAAACCTTGAAATACCTTTAAAATACGAGTGTGTTTGATCACTTTNATGTAATNAAGTTTTTACCAAAAAGAGGAATAGACTAGTAGTCTACACAACACCAAAACTCGCCTAATCTATTGACTTAATAAAAAGGCAGTACTAGTACACGCCCTTCCTNAAGCTTAGGCTACTTTCATCATCTCATCGCAGCCCTTGGCTCTAAAGCTCTCGTTAACGTCAACGTATGATACCATCATGAGGAATACCCTAACAACGTATATATCTCACNGGNCTTAAGCTATCCCATACATTAAAGATAACATTTAGTTTGTNGCNTTTGTCCCTTGGAGGGATCTTTTAACCTTTTTCCCGTNAAAGAACATCATCATANTCATATACATGAAGGGTTCAAATTTACTAGACTGGTTATTCTTTATTTCTTTCACATAAAAAAGGGGAAATACAATTACATTAAACATACGAATATATAATTAACATGACCAAGATTTTCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.566232
PRIMER_LEFT_0_PENALTY=3.417723
PRIMER_RIGHT_0_PENALTY=0.148509
PRIMER_LEFT_0_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_0_SEQUENCE=AAGTGGTTGTTGGCTCAAGG
PRIMER_LEFT_0=486,20
PRIMER_RIGHT_0=1427,20
PRIMER_LEFT_0_TM=56.582
PRIMER_RIGHT_0_TM=60.149
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=942
PRIMER_PAIR_1_PENALTY=4.173264
PRIMER_LEFT_1_PENALTY=3.417723
PRIMER_RIGHT_1_PENALTY=0.755541
PRIMER_LEFT_1_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_1_SEQUENCE=GCTCAAGGATCTCCCAAAAA
PRIMER_LEFT_1=486,20
PRIMER_RIGHT_1=1415,20
PRIMER_LEFT_1_TM=56.582
PRIMER_RIGHT_1_TM=59.244
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=930
PRIMER_PAIR_2_PENALTY=4.368425
PRIMER_LEFT_2_PENALTY=3.417723
PRIMER_RIGHT_2_PENALTY=0.950702
PRIMER_LEFT_2_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_2_SEQUENCE=TGAAATTGAATCGGGTGAGA
PRIMER_LEFT_2=486,20
PRIMER_RIGHT_2=1210,20
PRIMER_LEFT_2_TM=56.582
PRIMER_RIGHT_2_TM=59.049
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=725
PRIMER_PAIR_3_PENALTY=4.822470
PRIMER_LEFT_3_PENALTY=3.417723
PRIMER_RIGHT_3_PENALTY=1.404747
PRIMER_LEFT_3_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_3_SEQUENCE=TCGTGAAACTTTTAGTGGGGA
PRIMER_LEFT_3=486,20
PRIMER_RIGHT_3=1279,21
PRIMER_LEFT_3_TM=56.582
PRIMER_RIGHT_3_TM=59.595
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=794
PRIMER_PAIR_4_PENALTY=4.872136
PRIMER_LEFT_4_PENALTY=3.417723
PRIMER_RIGHT_4_PENALTY=1.454413
PRIMER_LEFT_4_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_4_SEQUENCE=TTGAAATTGAATCGGGTGAG
PRIMER_LEFT_4=486,20
PRIMER_RIGHT_4=1211,20
PRIMER_LEFT_4_TM=56.582
PRIMER_RIGHT_4_TM=58.546
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=726
PRIMER_PAIR_5_PENALTY=4.877726
PRIMER_LEFT_5_PENALTY=3.417723
PRIMER_RIGHT_5_PENALTY=1.460004
PRIMER_LEFT_5_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_5_SEQUENCE=GGCTCAAGGATCTCCCAAAA
PRIMER_LEFT_5=486,20
PRIMER_RIGHT_5=1416,20
PRIMER_LEFT_5_TM=56.582
PRIMER_RIGHT_5_TM=61.460
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=931
PRIMER_PAIR_6_PENALTY=4.997901
PRIMER_LEFT_6_PENALTY=3.417723
PRIMER_RIGHT_6_PENALTY=1.580178
PRIMER_LEFT_6_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_6_SEQUENCE=GTTGAAATTGAATCGGGTGAG
PRIMER_LEFT_6=486,20
PRIMER_RIGHT_6=1212,21
PRIMER_LEFT_6_TM=56.582
PRIMER_RIGHT_6_TM=59.420
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=727
PRIMER_PAIR_7_PENALTY=5.022175
PRIMER_LEFT_7_PENALTY=3.417723
PRIMER_RIGHT_7_PENALTY=1.604453
PRIMER_LEFT_7_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_7_SEQUENCE=GTTGAAATTGAATCGGGTGA
PRIMER_LEFT_7=486,20
PRIMER_RIGHT_7=1212,20
PRIMER_LEFT_7_TM=56.582
PRIMER_RIGHT_7_TM=58.396
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=727
PRIMER_PAIR_8_PENALTY=5.176987
PRIMER_LEFT_8_PENALTY=3.417723
PRIMER_RIGHT_8_PENALTY=1.759264
PRIMER_LEFT_8_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_8_SEQUENCE=TCGAGAATAACGGTACGGAA
PRIMER_LEFT_8=486,20
PRIMER_RIGHT_8=1380,20
PRIMER_LEFT_8_TM=56.582
PRIMER_RIGHT_8_TM=58.241
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=10.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=895
PRIMER_PAIR_9_PENALTY=5.183293
PRIMER_LEFT_9_PENALTY=3.417723
PRIMER_RIGHT_9_PENALTY=1.765570
PRIMER_LEFT_9_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_9_SEQUENCE=CTCGAGAATAACGGTACGGAA
PRIMER_LEFT_9=486,20
PRIMER_RIGHT_9=1381,21
PRIMER_LEFT_9_TM=56.582
PRIMER_RIGHT_9_TM=59.234
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=10.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=896
PRIMER_PAIR_10_PENALTY=5.300197
PRIMER_LEFT_10_PENALTY=3.417723
PRIMER_RIGHT_10_PENALTY=1.882474
PRIMER_LEFT_10_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_10_SEQUENCE=TTCGTGAAACTTTTAGTGGGG
PRIMER_LEFT_10=486,20
PRIMER_RIGHT_10=1280,21
PRIMER_LEFT_10_TM=56.582
PRIMER_RIGHT_10_TM=59.118
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=11.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=795
PRIMER_PAIR_11_PENALTY=5.438917
PRIMER_LEFT_11_PENALTY=3.417723
PRIMER_RIGHT_11_PENALTY=2.021195
PRIMER_LEFT_11_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_11_SEQUENCE=ACTCGAGAATAACGGTACGGAA
PRIMER_LEFT_11=486,20
PRIMER_RIGHT_11=1382,22
PRIMER_LEFT_11_TM=56.582
PRIMER_RIGHT_11_TM=60.021
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=10.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=897
PRIMER_PAIR_12_PENALTY=5.482129
PRIMER_LEFT_12_PENALTY=3.417723
PRIMER_RIGHT_12_PENALTY=2.064406
PRIMER_LEFT_12_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_12_SEQUENCE=TGAAACTTTTAGTGGGGATGG
PRIMER_LEFT_12=486,20
PRIMER_RIGHT_12=1276,21
PRIMER_LEFT_12_TM=56.582
PRIMER_RIGHT_12_TM=58.936
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=791
PRIMER_PAIR_13_PENALTY=5.654419
PRIMER_LEFT_13_PENALTY=3.417723
PRIMER_RIGHT_13_PENALTY=2.236697
PRIMER_LEFT_13_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_13_SEQUENCE=GAGAATAACGGTACGGAACCAA
PRIMER_LEFT_13=486,20
PRIMER_RIGHT_13=1378,22
PRIMER_LEFT_13_TM=56.582
PRIMER_RIGHT_13_TM=60.237
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=893
PRIMER_PAIR_14_PENALTY=5.676631
PRIMER_LEFT_14_PENALTY=3.417723
PRIMER_RIGHT_14_PENALTY=2.258908
PRIMER_LEFT_14_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_14_SEQUENCE=GTGAAACTTTTAGTGGGGATGG
PRIMER_LEFT_14=486,20
PRIMER_RIGHT_14=1277,22
PRIMER_LEFT_14_TM=56.582
PRIMER_RIGHT_14_TM=59.741
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=792
PRIMER_PAIR_15_PENALTY=5.687375
PRIMER_LEFT_15_PENALTY=3.417723
PRIMER_RIGHT_15_PENALTY=2.269653
PRIMER_LEFT_15_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_15_SEQUENCE=CGTGAAACTTTTAGTGGGGA
PRIMER_LEFT_15=486,20
PRIMER_RIGHT_15=1278,20
PRIMER_LEFT_15_TM=56.582
PRIMER_RIGHT_15_TM=57.730
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=10.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=793
PRIMER_PAIR_16_PENALTY=5.687375
PRIMER_LEFT_16_PENALTY=3.417723
PRIMER_RIGHT_16_PENALTY=2.269653
PRIMER_LEFT_16_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_16_SEQUENCE=TCGTGAAACTTTTAGTGGGG
PRIMER_LEFT_16=486,20
PRIMER_RIGHT_16=1279,20
PRIMER_LEFT_16_TM=56.582
PRIMER_RIGHT_16_TM=57.730
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=11.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=794
PRIMER_PAIR_17_PENALTY=5.717743
PRIMER_LEFT_17_PENALTY=3.417723
PRIMER_RIGHT_17_PENALTY=2.300021
PRIMER_LEFT_17_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_17_SEQUENCE=AAACAAGTGGTTGTTGGCTC
PRIMER_LEFT_17=486,20
PRIMER_RIGHT_17=1431,20
PRIMER_LEFT_17_TM=56.582
PRIMER_RIGHT_17_TM=57.700
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=946
PRIMER_PAIR_18_PENALTY=5.770118
PRIMER_LEFT_18_PENALTY=3.417723
PRIMER_RIGHT_18_PENALTY=2.352395
PRIMER_LEFT_18_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_18_SEQUENCE=AGTTGAAATTGAATCGGGTGAG
PRIMER_LEFT_18=486,20
PRIMER_RIGHT_18=1213,22
PRIMER_LEFT_18_TM=56.582
PRIMER_RIGHT_18_TM=60.352
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=728
PRIMER_PAIR_19_PENALTY=5.785353
PRIMER_LEFT_19_PENALTY=3.417723
PRIMER_RIGHT_19_PENALTY=2.367630
PRIMER_LEFT_19_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_19_SEQUENCE=TTTCGTGAAACTTTTAGTGGGG
PRIMER_LEFT_19=486,20
PRIMER_RIGHT_19=1281,22
PRIMER_LEFT_19_TM=56.582
PRIMER_RIGHT_19_TM=60.368
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=11.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=796
PRIMER_PAIR_20_PENALTY=5.803644
PRIMER_LEFT_20_PENALTY=3.417723
PRIMER_RIGHT_20_PENALTY=2.385922
PRIMER_LEFT_20_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_20_SEQUENCE=GAATAACGGTACGGAACCAATC
PRIMER_LEFT_20=486,20
PRIMER_RIGHT_20=1376,22
PRIMER_LEFT_20_TM=56.582
PRIMER_RIGHT_20_TM=59.614
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=891
PRIMER_PAIR_21_PENALTY=5.836592
PRIMER_LEFT_21_PENALTY=3.417723
PRIMER_RIGHT_21_PENALTY=2.418869
PRIMER_LEFT_21_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_21_SEQUENCE=GAAATTGAATCGGGTGAGAA
PRIMER_LEFT_21=486,20
PRIMER_RIGHT_21=1209,20
PRIMER_LEFT_21_TM=56.582
PRIMER_RIGHT_21_TM=57.581
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=724
PRIMER_PAIR_22_PENALTY=5.900377
PRIMER_LEFT_22_PENALTY=3.417723
PRIMER_RIGHT_22_PENALTY=2.482654
PRIMER_LEFT_22_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_22_SEQUENCE=AGTTGAAATTGAATCGGGTG
PRIMER_LEFT_22=486,20
PRIMER_RIGHT_22=1213,20
PRIMER_LEFT_22_TM=56.582
PRIMER_RIGHT_22_TM=57.517
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=728
PRIMER_PAIR_23_PENALTY=5.914482
PRIMER_LEFT_23_PENALTY=3.417723
PRIMER_RIGHT_23_PENALTY=2.496760
PRIMER_LEFT_23_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_23_SEQUENCE=AGAATAACGGTACGGAACCAA
PRIMER_LEFT_23=486,20
PRIMER_RIGHT_23=1377,21
PRIMER_LEFT_23_TM=56.582
PRIMER_RIGHT_23_TM=58.503
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=892
PRIMER_PAIR_24_PENALTY=5.915732
PRIMER_LEFT_24_PENALTY=3.417723
PRIMER_RIGHT_24_PENALTY=2.498009
PRIMER_LEFT_24_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_24_SEQUENCE=GTTGGCTCAAGGATCTCCCA
PRIMER_LEFT_24=486,20
PRIMER_RIGHT_24=1419,20
PRIMER_LEFT_24_TM=56.582
PRIMER_RIGHT_24_TM=62.498
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=9.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=934
PRIMER_PAIR_25_PENALTY=5.930734
PRIMER_LEFT_25_PENALTY=3.417723
PRIMER_RIGHT_25_PENALTY=2.513011
PRIMER_LEFT_25_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_25_SEQUENCE=GAATAACGGTACGGAACCAA
PRIMER_LEFT_25=486,20
PRIMER_RIGHT_25=1376,20
PRIMER_LEFT_25_TM=56.582
PRIMER_RIGHT_25_TM=57.487
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=891
PRIMER_PAIR_26_PENALTY=5.941983
PRIMER_LEFT_26_PENALTY=3.417723
PRIMER_RIGHT_26_PENALTY=2.524260
PRIMER_LEFT_26_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_26_SEQUENCE=CTTTTAGTGGGGATGGAAGAAA
PRIMER_LEFT_26=486,20
PRIMER_RIGHT_26=1271,22
PRIMER_LEFT_26_TM=56.582
PRIMER_RIGHT_26_TM=59.476
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=786
PRIMER_PAIR_27_PENALTY=5.961739
PRIMER_LEFT_27_PENALTY=3.417723
PRIMER_RIGHT_27_PENALTY=2.544017
PRIMER_LEFT_27_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_27_SEQUENCE=ATTGAATCGGGTGAGAAATG
PRIMER_LEFT_27=486,20
PRIMER_RIGHT_27=1206,20
PRIMER_LEFT_27_TM=56.582
PRIMER_RIGHT_27_TM=57.456
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=7.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=721
PRIMER_PAIR_28_PENALTY=6.067270
PRIMER_LEFT_28_PENALTY=3.417723
PRIMER_RIGHT_28_PENALTY=2.649547
PRIMER_LEFT_28_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_28_SEQUENCE=GAAACTTTTAGTGGGGATGGAA
PRIMER_LEFT_28=486,20
PRIMER_RIGHT_28=1275,22
PRIMER_LEFT_28_TM=56.582
PRIMER_RIGHT_28_TM=59.350
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=790
PRIMER_PAIR_29_PENALTY=6.081291
PRIMER_LEFT_29_PENALTY=3.417723
PRIMER_RIGHT_29_PENALTY=2.663568
PRIMER_LEFT_29_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_29_SEQUENCE=TGGGGATGGAAGAAATTAGACA
PRIMER_LEFT_29=486,20
PRIMER_RIGHT_29=1264,22
PRIMER_LEFT_29_TM=56.582
PRIMER_RIGHT_29_TM=60.664
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=779
PRIMER_PAIR_30_PENALTY=6.107163
PRIMER_LEFT_30_PENALTY=3.417723
PRIMER_RIGHT_30_PENALTY=2.689440
PRIMER_LEFT_30_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_30_SEQUENCE=GGGGATGGAAGAAATTAGACAA
PRIMER_LEFT_30=486,20
PRIMER_RIGHT_30=1263,22
PRIMER_LEFT_30_TM=56.582
PRIMER_RIGHT_30_TM=59.311
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=778
PRIMER_PAIR_31_PENALTY=6.110890
PRIMER_LEFT_31_PENALTY=3.417723
PRIMER_RIGHT_31_PENALTY=2.693167
PRIMER_LEFT_31_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_31_SEQUENCE=TGAAACTTTTAGTGGGGATGGA
PRIMER_LEFT_31=486,20
PRIMER_RIGHT_31=1276,22
PRIMER_LEFT_31_TM=56.582
PRIMER_RIGHT_31_TM=60.693
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=791
PRIMER_PAIR_32_PENALTY=6.158932
PRIMER_LEFT_32_PENALTY=3.417723
PRIMER_RIGHT_32_PENALTY=2.741209
PRIMER_LEFT_32_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_32_SEQUENCE=CGTGAAACTTTTAGTGGGGATG
PRIMER_LEFT_32=486,20
PRIMER_RIGHT_32=1278,22
PRIMER_LEFT_32_TM=56.582
PRIMER_RIGHT_32_TM=60.741
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=793
PRIMER_PAIR_33_PENALTY=6.242952
PRIMER_LEFT_33_PENALTY=3.417723
PRIMER_RIGHT_33_PENALTY=2.825229
PRIMER_LEFT_33_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_33_SEQUENCE=CTTTTAGTGGGGATGGAAGAA
PRIMER_LEFT_33=486,20
PRIMER_RIGHT_33=1271,21
PRIMER_LEFT_33_TM=56.582
PRIMER_RIGHT_33_TM=58.175
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=786
PRIMER_PAIR_34_PENALTY=6.266575
PRIMER_LEFT_34_PENALTY=3.417723
PRIMER_RIGHT_34_PENALTY=2.848852
PRIMER_LEFT_34_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_34_SEQUENCE=TTCGTGAAACTTTTAGTGGGGA
PRIMER_LEFT_34=486,20
PRIMER_RIGHT_34=1280,22
PRIMER_LEFT_34_TM=56.582
PRIMER_RIGHT_34_TM=60.849
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=10.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=795
PRIMER_PAIR_35_PENALTY=6.288169
PRIMER_LEFT_35_PENALTY=3.417723
PRIMER_RIGHT_35_PENALTY=2.870447
PRIMER_LEFT_35_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_35_SEQUENCE=TTTAGTGGGGATGGAAGAAA
PRIMER_LEFT_35=486,20
PRIMER_RIGHT_35=1269,20
PRIMER_LEFT_35_TM=56.582
PRIMER_RIGHT_35_TM=57.130
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=784
PRIMER_PAIR_36_PENALTY=6.288169
PRIMER_LEFT_36_PENALTY=3.417723
PRIMER_RIGHT_36_PENALTY=2.870447
PRIMER_LEFT_36_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_36_SEQUENCE=TTTTAGTGGGGATGGAAGAA
PRIMER_LEFT_36=486,20
PRIMER_RIGHT_36=1270,20
PRIMER_LEFT_36_TM=56.582
PRIMER_RIGHT_36_TM=57.130
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=785
PRIMER_PAIR_37_PENALTY=6.321836
PRIMER_LEFT_37_PENALTY=3.417723
PRIMER_RIGHT_37_PENALTY=2.904113
PRIMER_LEFT_37_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_37_SEQUENCE=TAACGGTACGGAACCAATCT
PRIMER_LEFT_37=486,20
PRIMER_RIGHT_37=1373,20
PRIMER_LEFT_37_TM=56.582
PRIMER_RIGHT_37_TM=57.096
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=888
PRIMER_PAIR_38_PENALTY=6.351737
PRIMER_LEFT_38_PENALTY=3.417723
PRIMER_RIGHT_38_PENALTY=2.934014
PRIMER_LEFT_38_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_38_SEQUENCE=TGGGGATGGAAGAAATTAGA
PRIMER_LEFT_38=486,20
PRIMER_RIGHT_38=1264,20
PRIMER_LEFT_38_TM=56.582
PRIMER_RIGHT_38_TM=57.066
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=6.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=779
PRIMER_PAIR_39_PENALTY=6.384794
PRIMER_LEFT_39_PENALTY=3.417723
PRIMER_RIGHT_39_PENALTY=2.967071
PRIMER_LEFT_39_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_39_SEQUENCE=GAAACTTTTAGTGGGGATGGA
PRIMER_LEFT_39=486,20
PRIMER_RIGHT_39=1275,21
PRIMER_LEFT_39_TM=56.582
PRIMER_RIGHT_39_TM=58.033
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=790
PRIMER_PAIR_40_PENALTY=6.394687
PRIMER_LEFT_40_PENALTY=3.417723
PRIMER_RIGHT_40_PENALTY=2.976964
PRIMER_LEFT_40_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_40_SEQUENCE=TGGCTCAAGGATCTCCCAAA
PRIMER_LEFT_40=486,20
PRIMER_RIGHT_40=1417,20
PRIMER_LEFT_40_TM=56.582
PRIMER_RIGHT_40_TM=62.977
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=8.8000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=932
PRIMER_PAIR_41_PENALTY=6.394687
PRIMER_LEFT_41_PENALTY=3.417723
PRIMER_RIGHT_41_PENALTY=2.976964
PRIMER_LEFT_41_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_41_SEQUENCE=TTGGCTCAAGGATCTCCCAA
PRIMER_LEFT_41=486,20
PRIMER_RIGHT_41=1418,20
PRIMER_LEFT_41_TM=56.582
PRIMER_RIGHT_41_TM=62.977
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=10.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=933
PRIMER_PAIR_42_PENALTY=6.416803
PRIMER_LEFT_42_PENALTY=3.417723
PRIMER_RIGHT_42_PENALTY=2.999081
PRIMER_LEFT_42_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_42_SEQUENCE=ACTTTTAGTGGGGATGGAAGAA
PRIMER_LEFT_42=486,20
PRIMER_RIGHT_42=1272,22
PRIMER_LEFT_42_TM=56.582
PRIMER_RIGHT_42_TM=59.001
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=787
PRIMER_PAIR_43_PENALTY=6.416803
PRIMER_LEFT_43_PENALTY=3.417723
PRIMER_RIGHT_43_PENALTY=2.999081
PRIMER_LEFT_43_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_43_SEQUENCE=AACTTTTAGTGGGGATGGAAGA
PRIMER_LEFT_43=486,20
PRIMER_RIGHT_43=1273,22
PRIMER_LEFT_43_TM=56.582
PRIMER_RIGHT_43_TM=59.001
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=788
PRIMER_PAIR_44_PENALTY=6.438151
PRIMER_LEFT_44_PENALTY=3.417723
PRIMER_RIGHT_44_PENALTY=3.020429
PRIMER_LEFT_44_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_44_SEQUENCE=GGGGATGGAAGAAATTAGACA
PRIMER_LEFT_44=486,20
PRIMER_RIGHT_44=1263,21
PRIMER_LEFT_44_TM=56.582
PRIMER_RIGHT_44_TM=57.980
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=778
PRIMER_PAIR_45_PENALTY=6.438151
PRIMER_LEFT_45_PENALTY=3.417723
PRIMER_RIGHT_45_PENALTY=3.020429
PRIMER_LEFT_45_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_45_SEQUENCE=TGGGGATGGAAGAAATTAGAC
PRIMER_LEFT_45=486,20
PRIMER_RIGHT_45=1264,21
PRIMER_LEFT_45_TM=56.582
PRIMER_RIGHT_45_TM=57.980
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=5.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=779
PRIMER_PAIR_46_PENALTY=6.438151
PRIMER_LEFT_46_PENALTY=3.417723
PRIMER_RIGHT_46_PENALTY=3.020429
PRIMER_LEFT_46_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_46_SEQUENCE=GTGGGGATGGAAGAAATTAGA
PRIMER_LEFT_46=486,20
PRIMER_RIGHT_46=1265,21
PRIMER_LEFT_46_TM=56.582
PRIMER_RIGHT_46_TM=57.980
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=780
PRIMER_PAIR_47_PENALTY=6.459845
PRIMER_LEFT_47_PENALTY=3.417723
PRIMER_RIGHT_47_PENALTY=3.042122
PRIMER_LEFT_47_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_47_SEQUENCE=AGTGGGGATGGAAGAAATTAGA
PRIMER_LEFT_47=486,20
PRIMER_RIGHT_47=1266,22
PRIMER_LEFT_47_TM=56.582
PRIMER_RIGHT_47_TM=58.958
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=781
PRIMER_PAIR_48_PENALTY=6.543564
PRIMER_LEFT_48_PENALTY=3.417723
PRIMER_RIGHT_48_PENALTY=3.125841
PRIMER_LEFT_48_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_48_SEQUENCE=AATAACGGTACGGAACCAATC
PRIMER_LEFT_48=486,20
PRIMER_RIGHT_48=1375,21
PRIMER_LEFT_48_TM=56.582
PRIMER_RIGHT_48_TM=57.874
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=890
PRIMER_PAIR_49_PENALTY=6.591813
PRIMER_LEFT_49_PENALTY=3.417723
PRIMER_RIGHT_49_PENALTY=3.174090
PRIMER_LEFT_49_SEQUENCE=CGAGGCATATTTTACAAGCA
PRIMER_RIGHT_49_SEQUENCE=GTGGGGATGGAAGAAATTAGAC
PRIMER_LEFT_49=486,20
PRIMER_RIGHT_49=1265,22
PRIMER_LEFT_49_TM=56.582
PRIMER_RIGHT_49_TM=58.826
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=5.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=780
=
SEQUENCE_ID=MAGIC.329_1_9566597_9567396_ws-0_kn-0_107237_38763_TRUE
SEQUENCE_TARGET=950,899
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAGATCTTTATTACTCTAATATGTCGCTTTAACTTTACCAGCTAGCTAATACTGTGATTGTTTAACATATAACGAATAAACATACATTAGGTTACTCAAATGAAAACAACCAACAACAAAATATATGACAAGAACGAGTTGTGAAAAAAGAGTAAGTGGAAGTGTGGAGGCACACATGNGTCGTGAACTCTTTGATATTGTGTTGTCTATGGACTTTCACAAACTCTACATACAAAATAAAAAAAACGAAGTAAGTGAAGTAAAATGCTTAATCTTGTAATTAATATCTTCTAACCAGCACAATTAATATTTGCACACACATGTGATTTGGGTATGCATGCAGCCACTATTTCNATGGATTGATTTTATATACGATAGGATACCTTTACATATGCACTAAAGCATAATCAAAGTCAGAACGTTAATTAACAAAGCTAAACTAGCAATAACAAATAAGTCGGTTTACCAAAACTAAACTGACATCCCAACAACATGATCGTTGTAAACTTGTAATCCACAATACTCTAACATCAATTAATGCCTTAATAGACACTTAGCNGTCGGACACTAAATTTATGTTATGATCATCAACGTAAGTGAACTATGAACATTTTCTATTTTGAAATTTATTTATACGACAACAGAGCAAGGCCGAGGGAGAAACCATGGANAAGGCTAGAATCAAGAAGGAAGAAACCACTGAGCATCGTTGAAGTTGAAGTTGGAGAGACCGTGGAAGCGTTTGACGGTCTTTGTTGCAAGATTGAAGACACCGATGTCGAGGCATGACAATTTATTGTGACAGATACGATCATCACGAGTGAAATAAATGGAGTTAGGCTCAATTCCAAGGGTATGGTCAGCAGGCACGGTAATACCCAAATCATAGAAGAGGGCCTCATCACCTAGAGAATGTACCTCAAGAAGCCGGGTGTCGAGGGTTTTCGGGTCAAGATCTTTAGGATCCCTCTTGTACAGATGGAACATCCTGTGACTTTCAGAGGTACCTAAATCGTACGTAAAGACAACCAAAACTTGACCTGCTCTTGTAACAGCAATGCCTTTGTTAGATGGGATAGACCTCTTTTGCTTCATACGTTCACGTTCCTTCATTGTCATACTTGGCATCCACATTGGAAGCCTATGTTTCTCGGATACATCCTTGAAACCATCTTCATCTTGTCCAGACAAATCTAAGTGTCGAATGAACTCACGGGTGGCATAGACGTAAAGATTGTAACCTTTCAGTACCATATCGTTTAAACCTCGTAACTCCCTCTGAACCTCGAGCTCTGTTGGAATCTCGCGGTAATGATCATCCCCTGTCCTGCAAAACCCTAGAAATGGGTACATTTCAAACTGCCACACAACAACGTAGTTTCCATTCTTGTCGTCAACCCACAAACGACCTCTCAGATCTTCGGCAGCATGAGGAANTTCATCACAAAATGGATCCTCATCATCCATTANCATATGCTTGACATTCTCCTCTCCTTCACGCCAAATCTTAAAATGGCTACCCTTAAATGATTCTAGTGGTGGAAGNTCGATCCTCTTCTCGCTAAATACATCTATAATATAGAGTTTCATTCGAGAATCTACCACCAGAAACCACTTACCTGAGCTTCCCAGGAATCGATACCCTGAAAAGTCACTCTTCGGCTCGTAAACCCTANCATCTNCTGGGTTGTAAATACGACAACCATACTCTTCTGCAATCAGCATCAGCAATGGAGATCCGGCTTTTGGCTGCAGAATCAAAGTCGAACGCAGAGACTTAACAGGCTCAAAATCAGGAATACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGTTCGTTTGAGCGTAGATCTCTCCATTATCTNGTCTNTNTTTTTTCTNTTAATGTCGTCNTTCGATAAGATTGAGGAGGCGTTAAGTTGCGCATATTGAAATGGAAGACATTATAGTTAGGAACCACCAGGCCCAGTAACAGCAAGAAATTATATTTTAACGGGTCTATGGCCCAATATACGACGTCCATCACGGATCGTTTCGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_19394525_19394774_oy-0_ler-0_2358_20291_TRUE
SEQUENCE_TARGET=950,349
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17194203_17194568_po-0_bur-0_15534_2020_TRUE
SEQUENCE_TARGET=950,465
SEQUENCE_TEMPLATE=GAGACAGGATNTAGAAGAAACTAAGCAAAAAGAAAGTACGGGTTTGGCCCGGACAAACNATAAAGACGCAGGAGAGGAGTTGGTTGAAACAGCCAAGAAGTTGGAGCAGGCGACGAAAGAGGCAGAGGATGCAAAGGCATTGGCTACAGCTTCTAGAGACGAGCTTAGAATGGCGAAGGAATTNTCTGAGCAAGCGAAAAGAGGAATGTCTACGATAGAGAGTCGGTTAGTAGAGGCGAAAAANGAAATGGAGGCTGCTCGGGCNTCAGAGAAGTTAGCCTTGGCTGCTATAAAAGCTCTGCAAGANACTGAATCTTCTCAGAGATTNGANGAGATTAATAATTCACCAAGAAGCATTATAATNTCTGTAGAGGAATACTACGAGCTAAGCAAGCAGGCACTCGAGTCTGAGGAGGAGGCAAACACAAGACTATCAGAGATTGTCTCTCAAATAGAGGTGGCNAAAGAGGAAGAGTCNAGAATCTTGGAGAAACTAGAGGAAGTGAATAGAGAAATGAGTGTAAGAAAAGCAGAGCTTAAAGAGGCTAATGGAAAAGCNGAAAAGGCTAGAGATGGGAAGTTGGGAATGGAGCAAGAGCTGAGAAAATGGAGGTCGGAAAATGGGAAGAGGAGAACAGACGAAGGCAGAGAGCCTGAGAAAAGNCCNACGAGGTCAAGCACCGANGGGAGAAACAAGGAGAATGGATTCGGGCAGTCGAAATCGTTTGCATTTGGTGANCAAGGAAGCAGCAGCAACNACNCTGGAGGAAGTACTACTACTANTAATAATAATNTGACTCCTGAAACNAAGAAGAAGAAGAAGAAGCTTTCTTTGTTTCCAAAAGTTTTCATGTTCTTGTCAAGAAAGAAGTCTCACAGTCACAAGTGAATAATGTTNCATACCTTTTTCTTTTTTCTTCAAAACATTTGGNTTGTTTTTTCNNTTCTTACACCAACAAACAATGCAGACTCCTTAAGTTTGGACANTTATTTTACAATGNATGATTNTGTTAATTTCACTCATAGGAAATAAAGNTANTGAATTGATACACANTTGCCAACANTNAGATGTTCAAAGANAAGATTCATATTATTAANACNCAATACCAATNTTTTTGTTNCAAAGANAAAAGANAATACAAAGATACNTTGAAGAGAACTTTNTTTCTGACTAGTTNTACTAAAAAGCCAACCCCTTTGANGACATAAATTGAGCTTGAGTTTAGGGTTTGCTGNATACCANTCTTTTTNGTGTTCACAACTTTGANGGNTTNGGGAAGATGTCAGGGGTNAATTTCTGNGCAGCACTGAGACTACCCTTGATCTTCATNGCACCCCTGCATCAACATTCAACNACAAACNACANGATTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCTAAACNTAGTTNTAGATTTTGTTGGTTCANANATAAGATAAAACTGCTAAACNNANTTCTGTAATGCAAAGNNAGTGACATATTTCAGGATTCAGANNNNNNCNTNTNGGACNAAATNCAAGTCNCACAAAAGCTTATANTGGTANATTGTGGTTCANACANAAGATAAAACTTTGGAGAAAGATTGAGTTTGTTGTCGGGNATGATNGATGTAGTGGTTCTGACACAGGATAAAACTGCTAAACATAGTTCTAGATCTTGTTGGTTCAGACATAAGATAAAACTGCTANACNTAGTTCTGTAATGCANAGNNAGTGACATANNTCNGGATTCAGANATCANCTTNTTGGACNAAANNCAAGTCANACAAAANCTTATANGCGGTAGATGTNNTTCAGACACAAGATANNNCTTTGGAGNAAGATTANGTTTGTTGTCGGGAATGNTGGATNTAGNGGTTNAGACACAGGACNAAACTGNTGNAACATACNANTNTAATGNAATNATNNNAAGNNAATGNNNGNAACAAACCTAATNAAAGCCATCTGAGGATTCATCTTNCCAGTNGCAACTTTGACAAAATCATCATCCTTGAAGGANAACGTAGCATCCACTTTCCCTCCTTCATATTTCCCTAATTTCCAAAGATCAAAANNATNAACGGAAAGTCAATAACTNAAAGCAANNNNNNNNNNNNNNNNNATTTCAATTCCCAATTGCCATAACCAANAAGAAAAACNTAACCTTTGATGCAAAANCAAATNTCANGATTCTCTCTTNTCTAAATNCNCAANTACTCTTCACNTTTGTCTCCAATCNAATT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=7.365204
PRIMER_LEFT_0_PENALTY=5.006651
PRIMER_RIGHT_0_PENALTY=2.358553
PRIMER_LEFT_0_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_0_SEQUENCE=CCCGACAACAAACTCAATCT
PRIMER_LEFT_0=860,25
PRIMER_RIGHT_0=1742,20
PRIMER_LEFT_0_TM=60.007
PRIMER_RIGHT_0_TM=57.641
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=883
PRIMER_PAIR_1_PENALTY=7.391298
PRIMER_LEFT_1_PENALTY=5.006651
PRIMER_RIGHT_1_PENALTY=2.384647
PRIMER_LEFT_1_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_1_SEQUENCE=CCCGACAACAAACTCAATCTTT
PRIMER_LEFT_1=860,25
PRIMER_RIGHT_1=1742,22
PRIMER_LEFT_1_TM=60.007
PRIMER_RIGHT_1_TM=60.385
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=883
PRIMER_PAIR_2_PENALTY=8.225186
PRIMER_LEFT_2_PENALTY=5.866633
PRIMER_RIGHT_2_PENALTY=2.358553
PRIMER_LEFT_2_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_2_SEQUENCE=CCCGACAACAAACTCAATCT
PRIMER_LEFT_2=856,25
PRIMER_RIGHT_2=1742,20
PRIMER_LEFT_2_TM=59.133
PRIMER_RIGHT_2_TM=57.641
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=887
PRIMER_PAIR_3_PENALTY=8.251280
PRIMER_LEFT_3_PENALTY=5.866633
PRIMER_RIGHT_3_PENALTY=2.384647
PRIMER_LEFT_3_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_3_SEQUENCE=CCCGACAACAAACTCAATCTTT
PRIMER_LEFT_3=856,25
PRIMER_RIGHT_3=1742,22
PRIMER_LEFT_3_TM=59.133
PRIMER_RIGHT_3_TM=60.385
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=887
PRIMER_PAIR_4_PENALTY=8.263809
PRIMER_LEFT_4_PENALTY=5.006651
PRIMER_RIGHT_4_PENALTY=3.257158
PRIMER_LEFT_4_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_4_SEQUENCE=CCGACAACAAACTCAATCTTTC
PRIMER_LEFT_4=860,25
PRIMER_RIGHT_4=1741,22
PRIMER_LEFT_4_TM=60.007
PRIMER_RIGHT_4_TM=58.743
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=882
PRIMER_PAIR_5_PENALTY=8.577515
PRIMER_LEFT_5_PENALTY=6.218961
PRIMER_RIGHT_5_PENALTY=2.358553
PRIMER_LEFT_5_SEQUENCE=TGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_5_SEQUENCE=CCCGACAACAAACTCAATCT
PRIMER_LEFT_5=857,24
PRIMER_RIGHT_5=1742,20
PRIMER_LEFT_5_TM=57.781
PRIMER_RIGHT_5_TM=57.641
PRIMER_LEFT_5_GC_PERCENT=41.667
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=886
PRIMER_PAIR_6_PENALTY=8.603608
PRIMER_LEFT_6_PENALTY=6.218961
PRIMER_RIGHT_6_PENALTY=2.384647
PRIMER_LEFT_6_SEQUENCE=TGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_6_SEQUENCE=CCCGACAACAAACTCAATCTTT
PRIMER_LEFT_6=857,24
PRIMER_RIGHT_6=1742,22
PRIMER_LEFT_6_TM=57.781
PRIMER_RIGHT_6_TM=60.385
PRIMER_LEFT_6_GC_PERCENT=41.667
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=886
PRIMER_PAIR_7_PENALTY=8.880752
PRIMER_LEFT_7_PENALTY=5.006651
PRIMER_RIGHT_7_PENALTY=3.874102
PRIMER_LEFT_7_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_7_SEQUENCE=TCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_7=860,25
PRIMER_RIGHT_7=1772,23
PRIMER_LEFT_7_TM=60.007
PRIMER_RIGHT_7_TM=59.126
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=913
PRIMER_PAIR_8_PENALTY=9.023688
PRIMER_LEFT_8_PENALTY=6.665135
PRIMER_RIGHT_8_PENALTY=2.358553
PRIMER_LEFT_8_SEQUENCE=AAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_8_SEQUENCE=CCCGACAACAAACTCAATCT
PRIMER_LEFT_8=861,25
PRIMER_RIGHT_8=1742,20
PRIMER_LEFT_8_TM=58.335
PRIMER_RIGHT_8_TM=57.641
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=882
PRIMER_PAIR_9_PENALTY=9.049782
PRIMER_LEFT_9_PENALTY=6.665135
PRIMER_RIGHT_9_PENALTY=2.384647
PRIMER_LEFT_9_SEQUENCE=AAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_9_SEQUENCE=CCCGACAACAAACTCAATCTTT
PRIMER_LEFT_9=861,25
PRIMER_RIGHT_9=1742,22
PRIMER_LEFT_9_TM=58.335
PRIMER_RIGHT_9_TM=60.385
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=882
PRIMER_PAIR_10_PENALTY=9.123791
PRIMER_LEFT_10_PENALTY=5.866633
PRIMER_RIGHT_10_PENALTY=3.257158
PRIMER_LEFT_10_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_10_SEQUENCE=CCGACAACAAACTCAATCTTTC
PRIMER_LEFT_10=856,25
PRIMER_RIGHT_10=1741,22
PRIMER_LEFT_10_TM=59.133
PRIMER_RIGHT_10_TM=58.743
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=886
PRIMER_PAIR_11_PENALTY=9.219479
PRIMER_LEFT_11_PENALTY=6.860925
PRIMER_RIGHT_11_PENALTY=2.358553
PRIMER_LEFT_11_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_11_SEQUENCE=CCCGACAACAAACTCAATCT
PRIMER_LEFT_11=860,26
PRIMER_RIGHT_11=1742,20
PRIMER_LEFT_11_TM=60.861
PRIMER_RIGHT_11_TM=57.641
PRIMER_LEFT_11_GC_PERCENT=42.308
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=883
PRIMER_PAIR_12_PENALTY=9.245572
PRIMER_LEFT_12_PENALTY=6.860925
PRIMER_RIGHT_12_PENALTY=2.384647
PRIMER_LEFT_12_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_12_SEQUENCE=CCCGACAACAAACTCAATCTTT
PRIMER_LEFT_12=860,26
PRIMER_RIGHT_12=1742,22
PRIMER_LEFT_12_TM=60.861
PRIMER_RIGHT_12_TM=60.385
PRIMER_LEFT_12_GC_PERCENT=42.308
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=883
PRIMER_PAIR_13_PENALTY=9.324427
PRIMER_LEFT_13_PENALTY=5.006651
PRIMER_RIGHT_13_PENALTY=4.317776
PRIMER_LEFT_13_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_13_SEQUENCE=TCCTGTGTCAGAACCACTACA
PRIMER_LEFT_13=860,25
PRIMER_RIGHT_13=1772,21
PRIMER_LEFT_13_TM=60.007
PRIMER_RIGHT_13_TM=56.682
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=5.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=913
PRIMER_PAIR_14_PENALTY=9.396391
PRIMER_LEFT_14_PENALTY=7.037837
PRIMER_RIGHT_14_PENALTY=2.358553
PRIMER_LEFT_14_SEQUENCE=AGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_14_SEQUENCE=CCCGACAACAAACTCAATCT
PRIMER_LEFT_14=862,24
PRIMER_RIGHT_14=1742,20
PRIMER_LEFT_14_TM=56.962
PRIMER_RIGHT_14_TM=57.641
PRIMER_LEFT_14_GC_PERCENT=41.667
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=881
PRIMER_PAIR_15_PENALTY=9.422485
PRIMER_LEFT_15_PENALTY=7.037837
PRIMER_RIGHT_15_PENALTY=2.384647
PRIMER_LEFT_15_SEQUENCE=AGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_15_SEQUENCE=CCCGACAACAAACTCAATCTTT
PRIMER_LEFT_15=862,24
PRIMER_RIGHT_15=1742,22
PRIMER_LEFT_15_TM=56.962
PRIMER_RIGHT_15_TM=60.385
PRIMER_LEFT_15_GC_PERCENT=41.667
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=881
PRIMER_PAIR_16_PENALTY=9.476120
PRIMER_LEFT_16_PENALTY=6.218961
PRIMER_RIGHT_16_PENALTY=3.257158
PRIMER_LEFT_16_SEQUENCE=TGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_16_SEQUENCE=CCGACAACAAACTCAATCTTTC
PRIMER_LEFT_16=857,24
PRIMER_RIGHT_16=1741,22
PRIMER_LEFT_16_TM=57.781
PRIMER_RIGHT_16_TM=58.743
PRIMER_LEFT_16_GC_PERCENT=41.667
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=885
PRIMER_PAIR_17_PENALTY=9.509402
PRIMER_LEFT_17_PENALTY=7.150848
PRIMER_RIGHT_17_PENALTY=2.358553
PRIMER_LEFT_17_SEQUENCE=GAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_17_SEQUENCE=CCCGACAACAAACTCAATCT
PRIMER_LEFT_17=863,23
PRIMER_RIGHT_17=1742,20
PRIMER_LEFT_17_TM=55.849
PRIMER_RIGHT_17_TM=57.641
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=880
PRIMER_PAIR_18_PENALTY=9.535495
PRIMER_LEFT_18_PENALTY=7.150848
PRIMER_RIGHT_18_PENALTY=2.384647
PRIMER_LEFT_18_SEQUENCE=GAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_18_SEQUENCE=CCCGACAACAAACTCAATCTTT
PRIMER_LEFT_18=863,23
PRIMER_RIGHT_18=1742,22
PRIMER_LEFT_18_TM=55.849
PRIMER_RIGHT_18_TM=60.385
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=880
PRIMER_PAIR_19_PENALTY=9.568571
PRIMER_LEFT_19_PENALTY=5.006651
PRIMER_RIGHT_19_PENALTY=4.561921
PRIMER_LEFT_19_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_19_SEQUENCE=ATCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_19=860,25
PRIMER_RIGHT_19=1773,24
PRIMER_LEFT_19_TM=60.007
PRIMER_RIGHT_19_TM=59.438
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.833
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=914
PRIMER_PAIR_20_PENALTY=9.740735
PRIMER_LEFT_20_PENALTY=5.866633
PRIMER_RIGHT_20_PENALTY=3.874102
PRIMER_LEFT_20_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_20_SEQUENCE=TCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_20=856,25
PRIMER_RIGHT_20=1772,23
PRIMER_LEFT_20_TM=59.133
PRIMER_RIGHT_20_TM=59.126
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=47.826
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.3000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=917
PRIMER_PAIR_21_PENALTY=9.844935
PRIMER_LEFT_21_PENALTY=5.006651
PRIMER_RIGHT_21_PENALTY=4.838284
PRIMER_LEFT_21_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_21_SEQUENCE=CCTGTGTCAGAACCACTACATC
PRIMER_LEFT_21=860,25
PRIMER_RIGHT_21=1771,22
PRIMER_LEFT_21_TM=60.007
PRIMER_RIGHT_21_TM=57.162
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.3000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=912
PRIMER_PAIR_22_PENALTY=9.844935
PRIMER_LEFT_22_PENALTY=5.006651
PRIMER_RIGHT_22_PENALTY=4.838284
PRIMER_LEFT_22_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_22_SEQUENCE=ATCCTGTGTCAGAACCACTACA
PRIMER_LEFT_22=860,25
PRIMER_RIGHT_22=1773,22
PRIMER_LEFT_22_TM=60.007
PRIMER_RIGHT_22_TM=57.162
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=5.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=914
PRIMER_PAIR_23_PENALTY=9.922293
PRIMER_LEFT_23_PENALTY=6.665135
PRIMER_RIGHT_23_PENALTY=3.257158
PRIMER_LEFT_23_SEQUENCE=AAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_23_SEQUENCE=CCGACAACAAACTCAATCTTTC
PRIMER_LEFT_23=861,25
PRIMER_RIGHT_23=1741,22
PRIMER_LEFT_23_TM=58.335
PRIMER_RIGHT_23_TM=58.743
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=881
PRIMER_PAIR_24_PENALTY=10.031304
PRIMER_LEFT_24_PENALTY=5.006651
PRIMER_RIGHT_24_PENALTY=5.024653
PRIMER_LEFT_24_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_24_SEQUENCE=TTTATCCTGTGTCAGAACCACTACA
PRIMER_LEFT_24=860,25
PRIMER_RIGHT_24=1776,25
PRIMER_LEFT_24_TM=60.007
PRIMER_RIGHT_24_TM=59.975
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=5.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=917
PRIMER_PAIR_25_PENALTY=10.062716
PRIMER_LEFT_25_PENALTY=5.006651
PRIMER_RIGHT_25_PENALTY=5.056065
PRIMER_LEFT_25_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_25_SEQUENCE=CCCGACAACAAACTCAATCTTTC
PRIMER_LEFT_25=860,25
PRIMER_RIGHT_25=1742,23
PRIMER_LEFT_25_TM=60.007
PRIMER_RIGHT_25_TM=62.056
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=883
PRIMER_PAIR_26_PENALTY=10.093063
PRIMER_LEFT_26_PENALTY=6.218961
PRIMER_RIGHT_26_PENALTY=3.874102
PRIMER_LEFT_26_SEQUENCE=TGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_26_SEQUENCE=TCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_26=857,24
PRIMER_RIGHT_26=1772,23
PRIMER_LEFT_26_TM=57.781
PRIMER_RIGHT_26_TM=59.126
PRIMER_LEFT_26_GC_PERCENT=41.667
PRIMER_RIGHT_26_GC_PERCENT=47.826
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.3000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=916
PRIMER_PAIR_27_PENALTY=10.118083
PRIMER_LEFT_27_PENALTY=6.860925
PRIMER_RIGHT_27_PENALTY=3.257158
PRIMER_LEFT_27_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_27_SEQUENCE=CCGACAACAAACTCAATCTTTC
PRIMER_LEFT_27=860,26
PRIMER_RIGHT_27=1741,22
PRIMER_LEFT_27_TM=60.861
PRIMER_RIGHT_27_TM=58.743
PRIMER_LEFT_27_GC_PERCENT=42.308
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=882
PRIMER_PAIR_28_PENALTY=10.184409
PRIMER_LEFT_28_PENALTY=5.866633
PRIMER_RIGHT_28_PENALTY=4.317776
PRIMER_LEFT_28_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_28_SEQUENCE=TCCTGTGTCAGAACCACTACA
PRIMER_LEFT_28=856,25
PRIMER_RIGHT_28=1772,21
PRIMER_LEFT_28_TM=59.133
PRIMER_RIGHT_28_TM=56.682
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=5.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=917
PRIMER_PAIR_29_PENALTY=10.214942
PRIMER_LEFT_29_PENALTY=5.006651
PRIMER_RIGHT_29_PENALTY=5.208292
PRIMER_LEFT_29_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_29_SEQUENCE=CAGTTTTATCCTGTGTCAGAACC
PRIMER_LEFT_29=860,25
PRIMER_RIGHT_29=1780,23
PRIMER_LEFT_29_TM=60.007
PRIMER_RIGHT_29_TM=57.792
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=43.478
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=921
PRIMER_PAIR_30_PENALTY=10.260315
PRIMER_LEFT_30_PENALTY=5.006651
PRIMER_RIGHT_30_PENALTY=5.253665
PRIMER_LEFT_30_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_30_SEQUENCE=GCAGTTTTATCCTGTGTCAGAACC
PRIMER_LEFT_30=860,25
PRIMER_RIGHT_30=1781,24
PRIMER_LEFT_30_TM=60.007
PRIMER_RIGHT_30_TM=61.254
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=45.833
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=922
PRIMER_PAIR_31_PENALTY=10.277085
PRIMER_LEFT_31_PENALTY=5.006651
PRIMER_RIGHT_31_PENALTY=5.270435
PRIMER_LEFT_31_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_31_SEQUENCE=TTTTATCCTGTGTCAGAACCAC
PRIMER_LEFT_31=860,25
PRIMER_RIGHT_31=1777,22
PRIMER_LEFT_31_TM=60.007
PRIMER_RIGHT_31_TM=56.730
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=918
PRIMER_PAIR_32_PENALTY=10.294996
PRIMER_LEFT_32_PENALTY=7.037837
PRIMER_RIGHT_32_PENALTY=3.257158
PRIMER_LEFT_32_SEQUENCE=AGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_32_SEQUENCE=CCGACAACAAACTCAATCTTTC
PRIMER_LEFT_32=862,24
PRIMER_RIGHT_32=1741,22
PRIMER_LEFT_32_TM=56.962
PRIMER_RIGHT_32_TM=58.743
PRIMER_LEFT_32_GC_PERCENT=41.667
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=880
PRIMER_PAIR_33_PENALTY=10.307324
PRIMER_LEFT_33_PENALTY=5.006651
PRIMER_RIGHT_33_PENALTY=5.300674
PRIMER_LEFT_33_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_33_SEQUENCE=TTATCCTGTGTCAGAACCACTACA
PRIMER_LEFT_33=860,25
PRIMER_RIGHT_33=1775,24
PRIMER_LEFT_33_TM=60.007
PRIMER_RIGHT_33_TM=58.699
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=41.667
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=916
PRIMER_PAIR_34_PENALTY=10.353419
PRIMER_LEFT_34_PENALTY=5.006651
PRIMER_RIGHT_34_PENALTY=5.346768
PRIMER_LEFT_34_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_34_SEQUENCE=CGACAACAAACTCAATCTTTCTCC
PRIMER_LEFT_34=860,25
PRIMER_RIGHT_34=1740,24
PRIMER_LEFT_34_TM=60.007
PRIMER_RIGHT_34_TM=61.347
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=41.667
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=7.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=881
PRIMER_PAIR_35_PENALTY=10.359562
PRIMER_LEFT_35_PENALTY=5.006651
PRIMER_RIGHT_35_PENALTY=5.352911
PRIMER_LEFT_35_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_35_SEQUENCE=GTTTTATCCTGTGTCAGAACCAC
PRIMER_LEFT_35=860,25
PRIMER_RIGHT_35=1778,23
PRIMER_LEFT_35_TM=60.007
PRIMER_RIGHT_35_TM=57.647
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=43.478
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=919
PRIMER_PAIR_36_PENALTY=10.375793
PRIMER_LEFT_36_PENALTY=5.006651
PRIMER_RIGHT_36_PENALTY=5.369142
PRIMER_LEFT_36_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_36_SEQUENCE=GTTTTATCCTGTGTCAGAACCACT
PRIMER_LEFT_36=860,25
PRIMER_RIGHT_36=1778,24
PRIMER_LEFT_36_TM=60.007
PRIMER_RIGHT_36_TM=58.631
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=41.667
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=919
PRIMER_PAIR_37_PENALTY=10.375793
PRIMER_LEFT_37_PENALTY=5.006651
PRIMER_RIGHT_37_PENALTY=5.369142
PRIMER_LEFT_37_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_37_SEQUENCE=AGTTTTATCCTGTGTCAGAACCAC
PRIMER_LEFT_37=860,25
PRIMER_RIGHT_37=1779,24
PRIMER_LEFT_37_TM=60.007
PRIMER_RIGHT_37_TM=58.631
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=41.667
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=920
PRIMER_PAIR_38_PENALTY=10.408006
PRIMER_LEFT_38_PENALTY=7.150848
PRIMER_RIGHT_38_PENALTY=3.257158
PRIMER_LEFT_38_SEQUENCE=GAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_38_SEQUENCE=CCGACAACAAACTCAATCTTTC
PRIMER_LEFT_38=863,23
PRIMER_RIGHT_38=1741,22
PRIMER_LEFT_38_TM=55.849
PRIMER_RIGHT_38_TM=58.743
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=879
PRIMER_PAIR_39_PENALTY=10.428554
PRIMER_LEFT_39_PENALTY=5.866633
PRIMER_RIGHT_39_PENALTY=4.561921
PRIMER_LEFT_39_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_39_SEQUENCE=ATCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_39=856,25
PRIMER_RIGHT_39=1773,24
PRIMER_LEFT_39_TM=59.133
PRIMER_RIGHT_39_TM=59.438
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.833
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.3000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=918
PRIMER_PAIR_40_PENALTY=10.474077
PRIMER_LEFT_40_PENALTY=5.006651
PRIMER_RIGHT_40_PENALTY=5.467426
PRIMER_LEFT_40_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_40_SEQUENCE=AGTTTTATCCTGTGTCAGAACCACT
PRIMER_LEFT_40=860,25
PRIMER_RIGHT_40=1779,25
PRIMER_LEFT_40_TM=60.007
PRIMER_RIGHT_40_TM=59.533
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=920
PRIMER_PAIR_41_PENALTY=10.536738
PRIMER_LEFT_41_PENALTY=6.218961
PRIMER_RIGHT_41_PENALTY=4.317776
PRIMER_LEFT_41_SEQUENCE=TGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_41_SEQUENCE=TCCTGTGTCAGAACCACTACA
PRIMER_LEFT_41=857,24
PRIMER_RIGHT_41=1772,21
PRIMER_LEFT_41_TM=57.781
PRIMER_RIGHT_41_TM=56.682
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=5.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=916
PRIMER_PAIR_42_PENALTY=10.538411
PRIMER_LEFT_42_PENALTY=5.006651
PRIMER_RIGHT_42_PENALTY=5.531761
PRIMER_LEFT_42_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_42_SEQUENCE=TATCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_42=860,25
PRIMER_RIGHT_42=1774,25
PRIMER_LEFT_42_TM=60.007
PRIMER_RIGHT_42_TM=59.468
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=44.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=915
PRIMER_PAIR_43_PENALTY=10.539236
PRIMER_LEFT_43_PENALTY=6.665135
PRIMER_RIGHT_43_PENALTY=3.874102
PRIMER_LEFT_43_SEQUENCE=AAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_43_SEQUENCE=TCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_43=861,25
PRIMER_RIGHT_43=1772,23
PRIMER_LEFT_43_TM=58.335
PRIMER_RIGHT_43_TM=59.126
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=47.826
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=912
PRIMER_PAIR_44_PENALTY=10.678485
PRIMER_LEFT_44_PENALTY=5.006651
PRIMER_RIGHT_44_PENALTY=5.671835
PRIMER_LEFT_44_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_44_SEQUENCE=TTTATCCTGTGTCAGAACCACT
PRIMER_LEFT_44=860,25
PRIMER_RIGHT_44=1776,22
PRIMER_LEFT_44_TM=60.007
PRIMER_RIGHT_44_TM=56.328
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=917
PRIMER_PAIR_45_PENALTY=10.704918
PRIMER_LEFT_45_PENALTY=5.866633
PRIMER_RIGHT_45_PENALTY=4.838284
PRIMER_LEFT_45_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_45_SEQUENCE=CCTGTGTCAGAACCACTACATC
PRIMER_LEFT_45=856,25
PRIMER_RIGHT_45=1771,22
PRIMER_LEFT_45_TM=59.133
PRIMER_RIGHT_45_TM=57.162
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.3000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=916
PRIMER_PAIR_46_PENALTY=10.704918
PRIMER_LEFT_46_PENALTY=5.866633
PRIMER_RIGHT_46_PENALTY=4.838284
PRIMER_LEFT_46_SEQUENCE=TTGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_46_SEQUENCE=ATCCTGTGTCAGAACCACTACA
PRIMER_LEFT_46=856,25
PRIMER_RIGHT_46=1773,22
PRIMER_LEFT_46_TM=59.133
PRIMER_RIGHT_46_TM=57.162
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=5.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=918
PRIMER_PAIR_47_PENALTY=10.723902
PRIMER_LEFT_47_PENALTY=5.006651
PRIMER_RIGHT_47_PENALTY=5.717252
PRIMER_LEFT_47_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAA
PRIMER_RIGHT_47_SEQUENCE=TATCCTGTGTCAGAACCACTACA
PRIMER_LEFT_47=860,25
PRIMER_RIGHT_47=1774,23
PRIMER_LEFT_47_TM=60.007
PRIMER_RIGHT_47_TM=57.283
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=5.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=915
PRIMER_PAIR_48_PENALTY=10.735027
PRIMER_LEFT_48_PENALTY=6.860925
PRIMER_RIGHT_48_PENALTY=3.874102
PRIMER_LEFT_48_SEQUENCE=CAAGAAAGAAGTCTCACAGTCACAAG
PRIMER_RIGHT_48_SEQUENCE=TCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_48=860,26
PRIMER_RIGHT_48=1772,23
PRIMER_LEFT_48_TM=60.861
PRIMER_RIGHT_48_TM=59.126
PRIMER_LEFT_48_GC_PERCENT=42.308
PRIMER_RIGHT_48_GC_PERCENT=47.826
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=913
PRIMER_PAIR_49_PENALTY=10.780882
PRIMER_LEFT_49_PENALTY=6.218961
PRIMER_RIGHT_49_PENALTY=4.561921
PRIMER_LEFT_49_SEQUENCE=TGTCAAGAAAGAAGTCTCACAGTC
PRIMER_RIGHT_49_SEQUENCE=ATCCTGTGTCAGAACCACTACATC
PRIMER_LEFT_49=857,24
PRIMER_RIGHT_49=1773,24
PRIMER_LEFT_49_TM=57.781
PRIMER_RIGHT_49_TM=59.438
PRIMER_LEFT_49_GC_PERCENT=41.667
PRIMER_RIGHT_49_GC_PERCENT=45.833
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.3000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=917
=
SEQUENCE_ID=MAGIC.338_2_6394203_6394338_ler-0_po-0_43957_156544_TRUE
SEQUENCE_TARGET=950,235
SEQUENCE_TEMPLATE=CTATCCNGANGTNNTANNANTTCGTAATATAGCGANTNAAGNNNNNNNTNTAAATTTTCTCGCTCTTTNGTCAANNGTTTCTAAGTATATACGTCANTAGTNTCATACCACAAATCNCTGAGCCTAGTAGATNTGNCNTCCNANCCTTACCTCAANCTCTTCTCAACNNAGTATGTACATGATTNNNNNNNGTAANTAACATGTGTGAATTTCGAAAACTAAATAAACNNAAAAACATGTTGTCAAANGCCGTTTACCGTTAGTCNAACAAGAAAAATAGAATCNGAAATNGAAACGAAAACGCAAACAACATNAGNANGCAACGACTACATAACATCCNNNNNATTGAANCAACCAAAAAAAANCCTAATTTACTAATCTAAGTCTTCCTCAACTTCAAATACCCTTCTCTTTTCATGGCCNTAANAGNTCAACAACAAAACATAGACAATCATCCAAATAAANTTCCGCGGAANCAATCCATCAGAGGAAGTGAGGACCGATGAGCTTTGCAATCTCTAGGAACCCAACCCGATCTCTTCCTTCGAATATCTTCTTCAGCTTCTCATCACACAGTATTTCTCTCTTGTTTTGTGGNTCCTNAAGAACAAAATTAGTCATTTNGTAAAACNNTGATGCANGTAAAACNTCTNNAAGTACCNTAGCTAAANTGGTTCNTNAAACTTTNAANCCTCAAGNCATGTCAATGAGAATAATNNNNANCTTTCTANTANAGTAAAGTTTTGGCTACTTGGTTGCTATAGACTNTAAATCTTGCAGAGAAACAGCNTCCACATTTAAANACTATANAACTAGAATTTCAAGAGNNNNNNNNNNNNNNNTTTCATANNNNNNNAACCTGAACCAAANANCAACAATANCAAGAAGCAGAGATCAAAANATCANNNNNNNNNNNCNAATGACANAACANANTAGGATTTTGGTCAAACTAGGCAANNAGGTTCAAGAACATAGNCCAGAGAANATTATTNNTNANCTCNAAGGATACACATAGCNNNNNNNNNNNNNNNNNNNNAATCAAAGAANNNNNNTTGCTTATACAAAANGTTATAAACAACTACCCCCAAAATCATATGAAGCAATTAGTCACNANNNTANTTNCANNTTCNTAATCNNAAGAAAANTTANAAGTTACANANCTAACAAGAAGACAANCTAATTCAATTTCTGTCTTCTTNAATCCAGACCGTTCAATTTTCCTNCAGAAACCCTAAAATNTCAAACCACCNGATTGAAGAAAGCGANACAAAACCNTAAGNGAAACCAANACCAGAGTGNTTCTGAGAGAGATTNTTGGTTGTTAAGTTACCTGAAGGTCATGTTCTTTGATGTAAGCCCAAATACGCTTNAGAGCTTGTGTGCGAGCNATNTCAGGAAGNTCGACGATGTCTTGCATCTCTGGTGAAACTGGTCTAGGTTTCATGATACCGCGTGGCACTCTCTTATTGGTTGCNGTTGGCTCTGAAGATTCCGTNGCAGATGTTACCGCTCGNACCAAGCNNNGTTTAGCCGCCGGGTGANAGGCGAACCGGAGAGAGGACAGAGGAGTTGANGGGTTGCGGAAAGATGTNGTTTTGATGCAGAGGAAAGTGGANAATGTTCCAGAAGAAACCGCCATTTTCGGTGTGCTTTGTCTGTGTGGTGGTNGAATTGGGAAGNNNAAAGGGGTTTTGTGTTATCCCTTTTGCTTTTTGGTTTTTNTTTTTTTTATTCTTAGTNTTGGNGTTTTGTCTTCATCGAGGAGAAACNCTATTTATTGTCTTTTNTTTTTTCTANTGGACAAATTGTTAANAGAAAACCTTNGGGAAANTTTTGGTTAAATACAAAATTTATGCGGGTTNAAANTATAAATATTTTTAGAATNTAAAANTTTATGNNAAAAAAANTAATTGACGGTCANAAAGANNNGAANATNTAAAATTAGAANTGAAAANTTCAANAAATTTNNAATAAAANNNATAAAATNGAGANATATCNAACTAACNNAAGNNNNNNNNNNNNNNNNNNNNNNTTGNTANAATGTANNNNNNNNNNNTTTGGCCANCANTCATANANATCNTNCTCTTAAGAAGCCAAACTTNANAAGCNTAGTANAACTTTTTAAANTATGCTTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.171631
PRIMER_LEFT_0_PENALTY=0.199316
PRIMER_RIGHT_0_PENALTY=0.972315
PRIMER_LEFT_0_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_0_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_0=486,20
PRIMER_RIGHT_0=1221,20
PRIMER_LEFT_0_TM=60.199
PRIMER_RIGHT_0_TM=59.028
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=736
PRIMER_PAIR_1_PENALTY=1.221342
PRIMER_LEFT_1_PENALTY=0.199316
PRIMER_RIGHT_1_PENALTY=1.022025
PRIMER_LEFT_1_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_1_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_1=486,20
PRIMER_RIGHT_1=1366,21
PRIMER_LEFT_1_TM=60.199
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=881
PRIMER_PAIR_2_PENALTY=1.544020
PRIMER_LEFT_2_PENALTY=0.832229
PRIMER_RIGHT_2_PENALTY=0.711791
PRIMER_LEFT_2_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_2_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_2=522,20
PRIMER_RIGHT_2=1494,20
PRIMER_LEFT_2_TM=60.832
PRIMER_RIGHT_2_TM=59.288
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=973
PRIMER_PAIR_3_PENALTY=1.546812
PRIMER_LEFT_3_PENALTY=0.199316
PRIMER_RIGHT_3_PENALTY=1.347496
PRIMER_LEFT_3_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_3_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_3=486,20
PRIMER_RIGHT_3=1366,20
PRIMER_LEFT_3_TM=60.199
PRIMER_RIGHT_3_TM=58.653
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=881
PRIMER_PAIR_4_PENALTY=1.577178
PRIMER_LEFT_4_PENALTY=0.199316
PRIMER_RIGHT_4_PENALTY=1.377862
PRIMER_LEFT_4_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_4_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_4=486,20
PRIMER_RIGHT_4=1367,21
PRIMER_LEFT_4_TM=60.199
PRIMER_RIGHT_4_TM=59.622
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=882
PRIMER_PAIR_5_PENALTY=1.670913
PRIMER_LEFT_5_PENALTY=0.959122
PRIMER_RIGHT_5_PENALTY=0.711791
PRIMER_LEFT_5_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_5_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_5=526,20
PRIMER_RIGHT_5=1494,20
PRIMER_LEFT_5_TM=60.959
PRIMER_RIGHT_5_TM=59.288
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=969
PRIMER_PAIR_6_PENALTY=1.718714
PRIMER_LEFT_6_PENALTY=0.746400
PRIMER_RIGHT_6_PENALTY=0.972315
PRIMER_LEFT_6_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_6_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_6=487,20
PRIMER_RIGHT_6=1221,20
PRIMER_LEFT_6_TM=59.254
PRIMER_RIGHT_6_TM=59.028
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=735
PRIMER_PAIR_7_PENALTY=1.718714
PRIMER_LEFT_7_PENALTY=0.746400
PRIMER_RIGHT_7_PENALTY=0.972315
PRIMER_LEFT_7_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_7_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_7=485,20
PRIMER_RIGHT_7=1221,20
PRIMER_LEFT_7_TM=59.254
PRIMER_RIGHT_7_TM=59.028
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=737
PRIMER_PAIR_8_PENALTY=1.757622
PRIMER_LEFT_8_PENALTY=1.045831
PRIMER_RIGHT_8_PENALTY=0.711791
PRIMER_LEFT_8_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_8_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_8=511,21
PRIMER_RIGHT_8=1494,20
PRIMER_LEFT_8_TM=59.954
PRIMER_RIGHT_8_TM=59.288
PRIMER_LEFT_8_GC_PERCENT=52.381
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=984
PRIMER_PAIR_9_PENALTY=1.768425
PRIMER_LEFT_9_PENALTY=0.746400
PRIMER_RIGHT_9_PENALTY=1.022025
PRIMER_LEFT_9_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_9_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_9=487,20
PRIMER_RIGHT_9=1366,21
PRIMER_LEFT_9_TM=59.254
PRIMER_RIGHT_9_TM=59.978
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=880
PRIMER_PAIR_10_PENALTY=1.768425
PRIMER_LEFT_10_PENALTY=0.746400
PRIMER_RIGHT_10_PENALTY=1.022025
PRIMER_LEFT_10_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_10_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_10=485,20
PRIMER_RIGHT_10=1366,21
PRIMER_LEFT_10_TM=59.254
PRIMER_RIGHT_10_TM=59.978
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.6000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=882
PRIMER_PAIR_11_PENALTY=1.769353
PRIMER_LEFT_11_PENALTY=0.199316
PRIMER_RIGHT_11_PENALTY=1.570037
PRIMER_LEFT_11_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_11_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_11=486,20
PRIMER_RIGHT_11=1427,21
PRIMER_LEFT_11_TM=60.199
PRIMER_RIGHT_11_TM=59.430
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=942
PRIMER_PAIR_12_PENALTY=1.773506
PRIMER_LEFT_12_PENALTY=0.801192
PRIMER_RIGHT_12_PENALTY=0.972315
PRIMER_LEFT_12_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_12_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_12=492,20
PRIMER_RIGHT_12=1221,20
PRIMER_LEFT_12_TM=60.801
PRIMER_RIGHT_12_TM=59.028
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=730
PRIMER_PAIR_13_PENALTY=1.823217
PRIMER_LEFT_13_PENALTY=0.801192
PRIMER_RIGHT_13_PENALTY=1.022025
PRIMER_LEFT_13_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_13_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_13=492,20
PRIMER_RIGHT_13=1366,21
PRIMER_LEFT_13_TM=60.801
PRIMER_RIGHT_13_TM=59.978
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=875
PRIMER_PAIR_14_PENALTY=1.854255
PRIMER_LEFT_14_PENALTY=0.832229
PRIMER_RIGHT_14_PENALTY=1.022025
PRIMER_LEFT_14_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_14_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_14=522,20
PRIMER_RIGHT_14=1366,21
PRIMER_LEFT_14_TM=60.832
PRIMER_RIGHT_14_TM=59.978
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=845
PRIMER_PAIR_15_PENALTY=1.899117
PRIMER_LEFT_15_PENALTY=1.187326
PRIMER_RIGHT_15_PENALTY=0.711791
PRIMER_LEFT_15_SEQUENCE=CAATCTCTAGGAACCCAACCC
PRIMER_RIGHT_15_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_15=512,21
PRIMER_RIGHT_15=1494,20
PRIMER_LEFT_15_TM=59.813
PRIMER_RIGHT_15_TM=59.288
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=983
PRIMER_PAIR_16_PENALTY=1.916583
PRIMER_LEFT_16_PENALTY=0.944268
PRIMER_RIGHT_16_PENALTY=0.972315
PRIMER_LEFT_16_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_16_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_16=483,20
PRIMER_RIGHT_16=1221,20
PRIMER_LEFT_16_TM=60.944
PRIMER_RIGHT_16_TM=59.028
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=739
PRIMER_PAIR_17_PENALTY=1.966294
PRIMER_LEFT_17_PENALTY=0.944268
PRIMER_RIGHT_17_PENALTY=1.022025
PRIMER_LEFT_17_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_17_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_17=483,20
PRIMER_RIGHT_17=1366,21
PRIMER_LEFT_17_TM=60.944
PRIMER_RIGHT_17_TM=59.978
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=884
PRIMER_PAIR_18_PENALTY=1.981148
PRIMER_LEFT_18_PENALTY=0.959122
PRIMER_RIGHT_18_PENALTY=1.022025
PRIMER_LEFT_18_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_18_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_18=526,20
PRIMER_RIGHT_18=1366,21
PRIMER_LEFT_18_TM=60.959
PRIMER_RIGHT_18_TM=59.978
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=841
PRIMER_PAIR_19_PENALTY=2.067857
PRIMER_LEFT_19_PENALTY=1.045831
PRIMER_RIGHT_19_PENALTY=1.022025
PRIMER_LEFT_19_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_19_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_19=511,21
PRIMER_RIGHT_19=1366,21
PRIMER_LEFT_19_TM=59.954
PRIMER_RIGHT_19_TM=59.978
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=856
PRIMER_PAIR_20_PENALTY=2.078032
PRIMER_LEFT_20_PENALTY=1.105717
PRIMER_RIGHT_20_PENALTY=0.972315
PRIMER_LEFT_20_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_20_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_20=491,20
PRIMER_RIGHT_20=1221,20
PRIMER_LEFT_20_TM=58.894
PRIMER_RIGHT_20_TM=59.028
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=731
PRIMER_PAIR_21_PENALTY=2.093896
PRIMER_LEFT_21_PENALTY=0.746400
PRIMER_RIGHT_21_PENALTY=1.347496
PRIMER_LEFT_21_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_21_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_21=487,20
PRIMER_RIGHT_21=1366,20
PRIMER_LEFT_21_TM=59.254
PRIMER_RIGHT_21_TM=58.653
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=880
PRIMER_PAIR_22_PENALTY=2.093896
PRIMER_LEFT_22_PENALTY=0.746400
PRIMER_RIGHT_22_PENALTY=1.347496
PRIMER_LEFT_22_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_22_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_22=485,20
PRIMER_RIGHT_22=1366,20
PRIMER_LEFT_22_TM=59.254
PRIMER_RIGHT_22_TM=58.653
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=882
PRIMER_PAIR_23_PENALTY=2.124261
PRIMER_LEFT_23_PENALTY=0.746400
PRIMER_RIGHT_23_PENALTY=1.377862
PRIMER_LEFT_23_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_23_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_23=487,20
PRIMER_RIGHT_23=1367,21
PRIMER_LEFT_23_TM=59.254
PRIMER_RIGHT_23_TM=59.622
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=881
PRIMER_PAIR_24_PENALTY=2.124261
PRIMER_LEFT_24_PENALTY=0.746400
PRIMER_RIGHT_24_PENALTY=1.377862
PRIMER_LEFT_24_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_24_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_24=485,20
PRIMER_RIGHT_24=1367,21
PRIMER_LEFT_24_TM=59.254
PRIMER_RIGHT_24_TM=59.622
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.6000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=883
PRIMER_PAIR_25_PENALTY=2.127742
PRIMER_LEFT_25_PENALTY=1.105717
PRIMER_RIGHT_25_PENALTY=1.022025
PRIMER_LEFT_25_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_25_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_25=491,20
PRIMER_RIGHT_25=1366,21
PRIMER_LEFT_25_TM=58.894
PRIMER_RIGHT_25_TM=59.978
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=876
PRIMER_PAIR_26_PENALTY=2.148688
PRIMER_LEFT_26_PENALTY=0.801192
PRIMER_RIGHT_26_PENALTY=1.347496
PRIMER_LEFT_26_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_26_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_26=492,20
PRIMER_RIGHT_26=1366,20
PRIMER_LEFT_26_TM=60.801
PRIMER_RIGHT_26_TM=58.653
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=875
PRIMER_PAIR_27_PENALTY=2.159641
PRIMER_LEFT_27_PENALTY=1.187326
PRIMER_RIGHT_27_PENALTY=0.972315
PRIMER_LEFT_27_SEQUENCE=CAATCTCTAGGAACCCAACCC
PRIMER_RIGHT_27_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_27=512,21
PRIMER_RIGHT_27=1221,20
PRIMER_LEFT_27_TM=59.813
PRIMER_RIGHT_27_TM=59.028
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=710
PRIMER_PAIR_28_PENALTY=2.179053
PRIMER_LEFT_28_PENALTY=0.801192
PRIMER_RIGHT_28_PENALTY=1.377862
PRIMER_LEFT_28_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_28_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_28=492,20
PRIMER_RIGHT_28=1367,21
PRIMER_LEFT_28_TM=60.801
PRIMER_RIGHT_28_TM=59.622
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=876
PRIMER_PAIR_29_PENALTY=2.179725
PRIMER_LEFT_29_PENALTY=0.832229
PRIMER_RIGHT_29_PENALTY=1.347496
PRIMER_LEFT_29_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_29_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_29=522,20
PRIMER_RIGHT_29=1366,20
PRIMER_LEFT_29_TM=60.832
PRIMER_RIGHT_29_TM=58.653
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=845
PRIMER_PAIR_30_PENALTY=2.209351
PRIMER_LEFT_30_PENALTY=1.187326
PRIMER_RIGHT_30_PENALTY=1.022025
PRIMER_LEFT_30_SEQUENCE=CAATCTCTAGGAACCCAACCC
PRIMER_RIGHT_30_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_30=512,21
PRIMER_RIGHT_30=1366,21
PRIMER_LEFT_30_TM=59.813
PRIMER_RIGHT_30_TM=59.978
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=855
PRIMER_PAIR_31_PENALTY=2.210091
PRIMER_LEFT_31_PENALTY=0.832229
PRIMER_RIGHT_31_PENALTY=1.377862
PRIMER_LEFT_31_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_31_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_31=522,20
PRIMER_RIGHT_31=1367,21
PRIMER_LEFT_31_TM=60.832
PRIMER_RIGHT_31_TM=59.622
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=846
PRIMER_PAIR_32_PENALTY=2.233540
PRIMER_LEFT_32_PENALTY=1.261226
PRIMER_RIGHT_32_PENALTY=0.972315
PRIMER_LEFT_32_SEQUENCE=AAGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_32_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_32=490,21
PRIMER_RIGHT_32=1221,20
PRIMER_LEFT_32_TM=60.261
PRIMER_RIGHT_32_TM=59.028
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=732
PRIMER_PAIR_33_PENALTY=2.283251
PRIMER_LEFT_33_PENALTY=1.261226
PRIMER_RIGHT_33_PENALTY=1.022025
PRIMER_LEFT_33_SEQUENCE=AAGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_33_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_33=490,21
PRIMER_RIGHT_33=1366,21
PRIMER_LEFT_33_TM=60.261
PRIMER_RIGHT_33_TM=59.978
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=877
PRIMER_PAIR_34_PENALTY=2.291764
PRIMER_LEFT_34_PENALTY=0.944268
PRIMER_RIGHT_34_PENALTY=1.347496
PRIMER_LEFT_34_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_34_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_34=483,20
PRIMER_RIGHT_34=1366,20
PRIMER_LEFT_34_TM=60.944
PRIMER_RIGHT_34_TM=58.653
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.6000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=884
PRIMER_PAIR_35_PENALTY=2.306618
PRIMER_LEFT_35_PENALTY=0.959122
PRIMER_RIGHT_35_PENALTY=1.347496
PRIMER_LEFT_35_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_35_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_35=526,20
PRIMER_RIGHT_35=1366,20
PRIMER_LEFT_35_TM=60.959
PRIMER_RIGHT_35_TM=58.653
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=841
PRIMER_PAIR_36_PENALTY=2.316437
PRIMER_LEFT_36_PENALTY=0.746400
PRIMER_RIGHT_36_PENALTY=1.570037
PRIMER_LEFT_36_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_36_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_36=487,20
PRIMER_RIGHT_36=1427,21
PRIMER_LEFT_36_TM=59.254
PRIMER_RIGHT_36_TM=59.430
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=941
PRIMER_PAIR_37_PENALTY=2.316437
PRIMER_LEFT_37_PENALTY=0.746400
PRIMER_RIGHT_37_PENALTY=1.570037
PRIMER_LEFT_37_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_37_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_37=485,20
PRIMER_RIGHT_37=1427,21
PRIMER_LEFT_37_TM=59.254
PRIMER_RIGHT_37_TM=59.430
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.6000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=943
PRIMER_PAIR_38_PENALTY=2.316463
PRIMER_LEFT_38_PENALTY=0.199316
PRIMER_RIGHT_38_PENALTY=2.117147
PRIMER_LEFT_38_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_38_SEQUENCE=TTCACCAGAGATGCAAGACA
PRIMER_LEFT_38=486,20
PRIMER_RIGHT_38=1426,20
PRIMER_LEFT_38_TM=60.199
PRIMER_RIGHT_38_TM=57.883
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=941
PRIMER_PAIR_39_PENALTY=2.322130
PRIMER_LEFT_39_PENALTY=0.944268
PRIMER_RIGHT_39_PENALTY=1.377862
PRIMER_LEFT_39_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_39_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_39=483,20
PRIMER_RIGHT_39=1367,21
PRIMER_LEFT_39_TM=60.944
PRIMER_RIGHT_39_TM=59.622
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=885
PRIMER_PAIR_40_PENALTY=2.336984
PRIMER_LEFT_40_PENALTY=0.959122
PRIMER_RIGHT_40_PENALTY=1.377862
PRIMER_LEFT_40_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_40_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_40=526,20
PRIMER_RIGHT_40=1367,21
PRIMER_LEFT_40_TM=60.959
PRIMER_RIGHT_40_TM=59.622
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=842
PRIMER_PAIR_41_PENALTY=2.371228
PRIMER_LEFT_41_PENALTY=0.801192
PRIMER_RIGHT_41_PENALTY=1.570037
PRIMER_LEFT_41_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_41_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_41=492,20
PRIMER_RIGHT_41=1427,21
PRIMER_LEFT_41_TM=60.801
PRIMER_RIGHT_41_TM=59.430
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=936
PRIMER_PAIR_42_PENALTY=2.393327
PRIMER_LEFT_42_PENALTY=1.045831
PRIMER_RIGHT_42_PENALTY=1.347496
PRIMER_LEFT_42_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_42_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_42=511,21
PRIMER_RIGHT_42=1366,20
PRIMER_LEFT_42_TM=59.954
PRIMER_RIGHT_42_TM=58.653
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=856
PRIMER_PAIR_43_PENALTY=2.402266
PRIMER_LEFT_43_PENALTY=0.832229
PRIMER_RIGHT_43_PENALTY=1.570037
PRIMER_LEFT_43_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_43_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_43=522,20
PRIMER_RIGHT_43=1427,21
PRIMER_LEFT_43_TM=60.832
PRIMER_RIGHT_43_TM=59.430
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=906
PRIMER_PAIR_44_PENALTY=2.414089
PRIMER_LEFT_44_PENALTY=1.702298
PRIMER_RIGHT_44_PENALTY=0.711791
PRIMER_LEFT_44_SEQUENCE=TGCAATCTCTAGGAACCCAA
PRIMER_RIGHT_44_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_44=510,20
PRIMER_RIGHT_44=1494,20
PRIMER_LEFT_44_TM=58.298
PRIMER_RIGHT_44_TM=59.288
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.0000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=985
PRIMER_PAIR_45_PENALTY=2.423693
PRIMER_LEFT_45_PENALTY=1.045831
PRIMER_RIGHT_45_PENALTY=1.377862
PRIMER_LEFT_45_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_45_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_45=511,21
PRIMER_RIGHT_45=1367,21
PRIMER_LEFT_45_TM=59.954
PRIMER_RIGHT_45_TM=59.622
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=857
PRIMER_PAIR_46_PENALTY=2.453213
PRIMER_LEFT_46_PENALTY=1.105717
PRIMER_RIGHT_46_PENALTY=1.347496
PRIMER_LEFT_46_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_46_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_46=491,20
PRIMER_RIGHT_46=1366,20
PRIMER_LEFT_46_TM=58.894
PRIMER_RIGHT_46_TM=58.653
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=876
PRIMER_PAIR_47_PENALTY=2.483579
PRIMER_LEFT_47_PENALTY=1.105717
PRIMER_RIGHT_47_PENALTY=1.377862
PRIMER_LEFT_47_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_47_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_47=491,20
PRIMER_RIGHT_47=1367,21
PRIMER_LEFT_47_TM=58.894
PRIMER_RIGHT_47_TM=59.622
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=877
PRIMER_PAIR_48_PENALTY=2.498214
PRIMER_LEFT_48_PENALTY=0.199316
PRIMER_RIGHT_48_PENALTY=2.298898
PRIMER_LEFT_48_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_48_SEQUENCE=GTTTCACCAGAGATGCAAGACA
PRIMER_LEFT_48=486,20
PRIMER_RIGHT_48=1428,22
PRIMER_LEFT_48_TM=60.199
PRIMER_RIGHT_48_TM=60.299
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=943
PRIMER_PAIR_49_PENALTY=2.514305
PRIMER_LEFT_49_PENALTY=0.944268
PRIMER_RIGHT_49_PENALTY=1.570037
PRIMER_LEFT_49_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_49_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_49=483,20
PRIMER_RIGHT_49=1427,21
PRIMER_LEFT_49_TM=60.944
PRIMER_RIGHT_49_TM=59.430
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=945
=
SEQUENCE_ID=MAGIC.175_5_16228762_16228823_can-0_rsch-4_252_3842879_TRUE
SEQUENCE_TARGET=950,161
SEQUENCE_TEMPLATE=TTGTTCTTCTCCNTNAAGTGAGANCCACAAATAGACNTAAAAAATATCTTGTGGTCAATNATCTGTNTCTCTANTCTCTGTTTCGTGTTCCANTTCACGANNNNNNNNNNACTTTNCTAAGTTTATNAAGAACATTTCTTTTCAATTTNTCGATTCTTTCTGGCTCATTCTCTCATCTCTNTTTCATATCNAAATTNTTGATTGAGAATTCGACTTGTGGAAATGGCAACAGTTGCATCAAATCTCNCTTGTATCGGAAAAGTAACGNTNTCTAGAGCTANTTCAATGNCAAGTCNTTTTTTNTATNATGCTNGCCAAAGAANTGGNATAAAGAGTGGATTTGGAATGGATTTTGCAGGTAGGANGGAGGTTGCAGATAAATTGCAGAAAGAAAGAGAAAGGAAGAGATCAGAGTCATTATCCTTACAAAGTCATTGAAATCACTCCTCCTCCTAAATCTCTCGGCATTCGTTGCCTTCCTCATGTGAGTCTCAAATCTTTCTCAATGCTCAACTACACTTCATAAGNATTTCANTACAGATTCCAAGTGAGAATGAGAAGCTTTTTTGATTTGCAGAANCTTCANTGTGGAGAGAACGTGATGATCGAAGGGCAAACGTACACAATCTCNGCNGTNACTCANCGGTATCAGCTCCGGAAAGGCAAATACGAGCCGAGTGAGAGGCGGCTCGATGTTCTCTCTGCNGCTAGATACGTTTTAAACCTTTACTTCGACAATTTGNTCCAAAACTCNTGNNAACTTTNATTTAGCNAAAANGTTTGGTCTANNNGATTTTCATTGTATCCATGAATGCTTGTGAATTTGTTAAGCAATNATTTGTANTTCTCTTAATCATTNCACNTTNTATCNCNNATATNNNNCAANNTNNTNGNNTTACNTNTNGNAANNNNNNNNNNNNTANTTTTGNNNATTNNTNNNNNATNNACNANNNATTCNTGNTTTNNCNNAANAANNTNAANACNNANTNNTNNNAANTNNNTTAATGATTTAACNATNCAACAACNCCCCAAAGTTCTTTNTAAAGGAAACNACGACTTCGNTAAACTACCAGGTTAAGGAAATGTATATAGCTAAGAANTCAAGCCAANTGCGTAAATTCTNCATCTTACGCCTCATGGTTTAGCTTCTATAATAAACAAACATTTTGAGTCATCNTCAGGCAATTAGCTTGCAAANAAAATGATATAATACGTCTAATTGCTNCCATAANTTTACAATTTAGTTGACCAACGTNGCTTGATTTAACTAAAACAACTGTCTGTCACCNAACAAACCAAGACGGACGAGTAGATTTGTATGTAANCTCTANACGAATCCGTTTGTGANCTTNATTTGCGTCACCGCCTTGTCACATTCGTGTGGACGCCTCACGACGTGTTTGCATAATGTTANTGAAACAGAAATATTNCACTCTTCCTCCANTTTTTGATTACTTCCATTTCCTCAAAAGTCAAAGCTCTCTTTCTTCTCTCTCGAGATTTGCTAACTTGTCAATGGCGGATGTTGAGAATCTGAACTCTGTTNCGGCGTCGGAAGACACAGAGTATGGATTTAAACGGCCGGAGATGTACAGCACAAACATAGCCAATTCCATAACTTCCTACGCTCGTCATGTTTTTGTTCTTTACAAAACTCCTGAAGCTTGGCTCTCTCACGTCGAAGAAGAAGGTTTACCTCAACGTTTCGCTACGTTGCTTAAAGATCGCAAATCTGATCTTCTTGTTCAGGTTTATACTAAAACGTTTGACATTTTCTTACGTTGTATCTCTTTCNGATGAAGATTCTCATTAGTCATTACGTTGACTTTTTTTNTTGTTGTTGTTGTAGACGAAGCTCAATGTATGTGAAGGTGGTGGATCNGACGGTGATGTTTTGATTTTTCCCGATATGATCAGATACAAGTGAGTGTGCTTAAGTTTTGTTTTTGTTAGCTTAAGATTTTTGGAAAANAAATAGTTGACTAGAATAGGATTTTGACTCTNAGCTAGATTTATAGTGTGTAAATTGTNGTATAAGCTAGTTTTAGAAACTTTAGAAGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.172529
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=0.099578
PRIMER_LEFT_0_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_0_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_0=593,20
PRIMER_RIGHT_0=1533,20
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=59.900
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=941
PRIMER_PAIR_1_PENALTY=0.221461
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_1_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_1=593,20
PRIMER_RIGHT_1=1308,20
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=716
PRIMER_PAIR_2_PENALTY=0.238665
PRIMER_LEFT_2_PENALTY=0.090155
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_2_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_2=447,20
PRIMER_RIGHT_2=1308,20
PRIMER_LEFT_2_TM=59.910
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=862
PRIMER_PAIR_3_PENALTY=0.362015
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_3_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_3=454,20
PRIMER_RIGHT_3=1308,20
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=855
PRIMER_PAIR_4_PENALTY=0.450783
PRIMER_LEFT_4_PENALTY=0.072951
PRIMER_RIGHT_4_PENALTY=0.377832
PRIMER_LEFT_4_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_4_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_4=593,20
PRIMER_RIGHT_4=1309,20
PRIMER_LEFT_4_TM=60.073
PRIMER_RIGHT_4_TM=59.622
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=717
PRIMER_PAIR_5_PENALTY=0.457878
PRIMER_LEFT_5_PENALTY=0.309369
PRIMER_RIGHT_5_PENALTY=0.148509
PRIMER_LEFT_5_SEQUENCE=TTGGAATGGATTTTGCAGGT
PRIMER_RIGHT_5_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_5=340,20
PRIMER_RIGHT_5=1308,20
PRIMER_LEFT_5_TM=60.309
PRIMER_RIGHT_5_TM=60.149
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=969
PRIMER_PAIR_6_PENALTY=0.467987
PRIMER_LEFT_6_PENALTY=0.090155
PRIMER_RIGHT_6_PENALTY=0.377832
PRIMER_LEFT_6_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_6_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_6=447,20
PRIMER_RIGHT_6=1309,20
PRIMER_LEFT_6_TM=59.910
PRIMER_RIGHT_6_TM=59.622
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=11.7000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=863
PRIMER_PAIR_7_PENALTY=0.569563
PRIMER_LEFT_7_PENALTY=0.469985
PRIMER_RIGHT_7_PENALTY=0.099578
PRIMER_LEFT_7_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_7_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_7=598,20
PRIMER_RIGHT_7=1533,20
PRIMER_LEFT_7_TM=60.470
PRIMER_RIGHT_7_TM=59.900
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=936
PRIMER_PAIR_8_PENALTY=0.591338
PRIMER_LEFT_8_PENALTY=0.213506
PRIMER_RIGHT_8_PENALTY=0.377832
PRIMER_LEFT_8_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_8_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_8=454,20
PRIMER_RIGHT_8=1309,20
PRIMER_LEFT_8_TM=60.214
PRIMER_RIGHT_8_TM=59.622
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.7000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=856
PRIMER_PAIR_9_PENALTY=0.618494
PRIMER_LEFT_9_PENALTY=0.469985
PRIMER_RIGHT_9_PENALTY=0.148509
PRIMER_LEFT_9_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_9_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_9=598,20
PRIMER_RIGHT_9=1308,20
PRIMER_LEFT_9_TM=60.470
PRIMER_RIGHT_9_TM=60.149
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=711
PRIMER_PAIR_10_PENALTY=0.643006
PRIMER_LEFT_10_PENALTY=0.072951
PRIMER_RIGHT_10_PENALTY=0.570054
PRIMER_LEFT_10_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_10_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_10=593,20
PRIMER_RIGHT_10=1384,20
PRIMER_LEFT_10_TM=60.073
PRIMER_RIGHT_10_TM=60.570
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=792
PRIMER_PAIR_11_PENALTY=0.645988
PRIMER_LEFT_11_PENALTY=0.546410
PRIMER_RIGHT_11_PENALTY=0.099578
PRIMER_LEFT_11_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_11_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_11=665,20
PRIMER_RIGHT_11=1533,20
PRIMER_LEFT_11_TM=59.454
PRIMER_RIGHT_11_TM=59.900
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=869
PRIMER_PAIR_12_PENALTY=0.660210
PRIMER_LEFT_12_PENALTY=0.090155
PRIMER_RIGHT_12_PENALTY=0.570054
PRIMER_LEFT_12_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_12_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_12=447,20
PRIMER_RIGHT_12=1384,20
PRIMER_LEFT_12_TM=59.910
PRIMER_RIGHT_12_TM=60.570
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=11.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=938
PRIMER_PAIR_13_PENALTY=0.681386
PRIMER_LEFT_13_PENALTY=0.581808
PRIMER_RIGHT_13_PENALTY=0.099578
PRIMER_LEFT_13_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_13_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_13=650,20
PRIMER_RIGHT_13=1533,20
PRIMER_LEFT_13_TM=60.582
PRIMER_RIGHT_13_TM=59.900
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=5.6000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=884
PRIMER_PAIR_14_PENALTY=0.687201
PRIMER_LEFT_14_PENALTY=0.309369
PRIMER_RIGHT_14_PENALTY=0.377832
PRIMER_LEFT_14_SEQUENCE=TTGGAATGGATTTTGCAGGT
PRIMER_RIGHT_14_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_14=340,20
PRIMER_RIGHT_14=1309,20
PRIMER_LEFT_14_TM=60.309
PRIMER_RIGHT_14_TM=59.622
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=970
PRIMER_PAIR_15_PENALTY=0.694919
PRIMER_LEFT_15_PENALTY=0.546410
PRIMER_RIGHT_15_PENALTY=0.148509
PRIMER_LEFT_15_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_15_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_15=665,20
PRIMER_RIGHT_15=1308,20
PRIMER_LEFT_15_TM=59.454
PRIMER_RIGHT_15_TM=60.149
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=644
PRIMER_PAIR_16_PENALTY=0.730317
PRIMER_LEFT_16_PENALTY=0.581808
PRIMER_RIGHT_16_PENALTY=0.148509
PRIMER_LEFT_16_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_16_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_16=650,20
PRIMER_RIGHT_16=1308,20
PRIMER_LEFT_16_TM=60.582
PRIMER_RIGHT_16_TM=60.149
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.6000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=659
PRIMER_PAIR_17_PENALTY=0.924242
PRIMER_LEFT_17_PENALTY=0.546410
PRIMER_RIGHT_17_PENALTY=0.377832
PRIMER_LEFT_17_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_17_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_17=665,20
PRIMER_RIGHT_17=1309,20
PRIMER_LEFT_17_TM=59.454
PRIMER_RIGHT_17_TM=59.622
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=645
PRIMER_PAIR_18_PENALTY=0.955163
PRIMER_LEFT_18_PENALTY=0.806653
PRIMER_RIGHT_18_PENALTY=0.148509
PRIMER_LEFT_18_SEQUENCE=TTTGGAATGGATTTTGCAGG
PRIMER_RIGHT_18_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_18=339,20
PRIMER_RIGHT_18=1308,20
PRIMER_LEFT_18_TM=60.807
PRIMER_RIGHT_18_TM=60.149
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=970
PRIMER_PAIR_19_PENALTY=0.959640
PRIMER_LEFT_19_PENALTY=0.581808
PRIMER_RIGHT_19_PENALTY=0.377832
PRIMER_LEFT_19_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_19_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_19=650,20
PRIMER_RIGHT_19=1309,20
PRIMER_LEFT_19_TM=60.582
PRIMER_RIGHT_19_TM=59.622
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=660
PRIMER_PAIR_20_PENALTY=1.034004
PRIMER_LEFT_20_PENALTY=0.934426
PRIMER_RIGHT_20_PENALTY=0.099578
PRIMER_LEFT_20_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_20_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_20=662,20
PRIMER_RIGHT_20=1533,20
PRIMER_LEFT_20_TM=60.934
PRIMER_RIGHT_20_TM=59.900
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=872
PRIMER_PAIR_21_PENALTY=1.040039
PRIMER_LEFT_21_PENALTY=0.469985
PRIMER_RIGHT_21_PENALTY=0.570054
PRIMER_LEFT_21_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_21_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_21=598,20
PRIMER_RIGHT_21=1384,20
PRIMER_LEFT_21_TM=60.470
PRIMER_RIGHT_21_TM=60.570
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=787
PRIMER_PAIR_22_PENALTY=1.075195
PRIMER_LEFT_22_PENALTY=0.975617
PRIMER_RIGHT_22_PENALTY=0.099578
PRIMER_LEFT_22_SEQUENCE=CAAATACGAGCCGAGTGAGA
PRIMER_RIGHT_22_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_22=663,20
PRIMER_RIGHT_22=1533,20
PRIMER_LEFT_22_TM=59.024
PRIMER_RIGHT_22_TM=59.900
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=871
PRIMER_PAIR_23_PENALTY=1.082935
PRIMER_LEFT_23_PENALTY=0.934426
PRIMER_RIGHT_23_PENALTY=0.148509
PRIMER_LEFT_23_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_23_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_23=662,20
PRIMER_RIGHT_23=1308,20
PRIMER_LEFT_23_TM=60.934
PRIMER_RIGHT_23_TM=60.149
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=647
PRIMER_PAIR_24_PENALTY=1.106758
PRIMER_LEFT_24_PENALTY=0.072951
PRIMER_RIGHT_24_PENALTY=1.033807
PRIMER_LEFT_24_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_24_SEQUENCE=CGGCCGTTTAAATCCATACT
PRIMER_LEFT_24=593,20
PRIMER_RIGHT_24=1583,20
PRIMER_LEFT_24_TM=60.073
PRIMER_RIGHT_24_TM=58.966
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=5.3000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=991
PRIMER_PAIR_25_PENALTY=1.116465
PRIMER_LEFT_25_PENALTY=0.546410
PRIMER_RIGHT_25_PENALTY=0.570054
PRIMER_LEFT_25_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_25_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_25=665,20
PRIMER_RIGHT_25=1384,20
PRIMER_LEFT_25_TM=59.454
PRIMER_RIGHT_25_TM=60.570
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=720
PRIMER_PAIR_26_PENALTY=1.122366
PRIMER_LEFT_26_PENALTY=1.022788
PRIMER_RIGHT_26_PENALTY=0.099578
PRIMER_LEFT_26_SEQUENCE=CAAATACGAGCCGAGTGAGAG
PRIMER_RIGHT_26_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_26=663,21
PRIMER_RIGHT_26=1533,20
PRIMER_LEFT_26_TM=60.023
PRIMER_RIGHT_26_TM=59.900
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=871
PRIMER_PAIR_27_PENALTY=1.124126
PRIMER_LEFT_27_PENALTY=0.975617
PRIMER_RIGHT_27_PENALTY=0.148509
PRIMER_LEFT_27_SEQUENCE=CAAATACGAGCCGAGTGAGA
PRIMER_RIGHT_27_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_27=663,20
PRIMER_RIGHT_27=1308,20
PRIMER_LEFT_27_TM=59.024
PRIMER_RIGHT_27_TM=60.149
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=646
PRIMER_PAIR_28_PENALTY=1.151862
PRIMER_LEFT_28_PENALTY=0.581808
PRIMER_RIGHT_28_PENALTY=0.570054
PRIMER_LEFT_28_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_28_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_28=650,20
PRIMER_RIGHT_28=1384,20
PRIMER_LEFT_28_TM=60.582
PRIMER_RIGHT_28_TM=60.570
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=5.6000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=735
PRIMER_PAIR_29_PENALTY=1.171297
PRIMER_LEFT_29_PENALTY=1.022788
PRIMER_RIGHT_29_PENALTY=0.148509
PRIMER_LEFT_29_SEQUENCE=CAAATACGAGCCGAGTGAGAG
PRIMER_RIGHT_29_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_29=663,21
PRIMER_RIGHT_29=1308,20
PRIMER_LEFT_29_TM=60.023
PRIMER_RIGHT_29_TM=60.149
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=646
PRIMER_PAIR_30_PENALTY=1.184485
PRIMER_LEFT_30_PENALTY=0.806653
PRIMER_RIGHT_30_PENALTY=0.377832
PRIMER_LEFT_30_SEQUENCE=TTTGGAATGGATTTTGCAGG
PRIMER_RIGHT_30_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_30=339,20
PRIMER_RIGHT_30=1309,20
PRIMER_LEFT_30_TM=60.807
PRIMER_RIGHT_30_TM=59.622
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=971
PRIMER_PAIR_31_PENALTY=1.202852
PRIMER_LEFT_31_PENALTY=1.054343
PRIMER_RIGHT_31_PENALTY=0.148509
PRIMER_LEFT_31_SEQUENCE=TGGAATGGATTTTGCAGGTAG
PRIMER_RIGHT_31_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_31=341,21
PRIMER_RIGHT_31=1308,20
PRIMER_LEFT_31_TM=59.946
PRIMER_RIGHT_31_TM=60.149
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=968
PRIMER_PAIR_32_PENALTY=1.232417
PRIMER_LEFT_32_PENALTY=0.072951
PRIMER_RIGHT_32_PENALTY=1.159466
PRIMER_LEFT_32_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_32_SEQUENCE=TCAGATTCTCAACATCCGCC
PRIMER_LEFT_32=593,20
PRIMER_RIGHT_32=1536,20
PRIMER_LEFT_32_TM=60.073
PRIMER_RIGHT_32_TM=61.159
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=12.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=944
PRIMER_PAIR_33_PENALTY=1.235607
PRIMER_LEFT_33_PENALTY=1.136029
PRIMER_RIGHT_33_PENALTY=0.099578
PRIMER_LEFT_33_SEQUENCE=AAGGCAAATACGAGCCGAGT
PRIMER_RIGHT_33_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_33=659,20
PRIMER_RIGHT_33=1533,20
PRIMER_LEFT_33_TM=61.136
PRIMER_RIGHT_33_TM=59.900
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=875
PRIMER_PAIR_34_PENALTY=1.284538
PRIMER_LEFT_34_PENALTY=1.136029
PRIMER_RIGHT_34_PENALTY=0.148509
PRIMER_LEFT_34_SEQUENCE=AAGGCAAATACGAGCCGAGT
PRIMER_RIGHT_34_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_34=659,20
PRIMER_RIGHT_34=1308,20
PRIMER_LEFT_34_TM=61.136
PRIMER_RIGHT_34_TM=60.149
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=650
PRIMER_PAIR_35_PENALTY=1.290653
PRIMER_LEFT_35_PENALTY=0.072951
PRIMER_RIGHT_35_PENALTY=1.217702
PRIMER_LEFT_35_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_35_SEQUENCE=CTACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_35=593,20
PRIMER_RIGHT_35=1311,21
PRIMER_LEFT_35_TM=60.073
PRIMER_RIGHT_35_TM=59.782
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=719
PRIMER_PAIR_36_PENALTY=1.300209
PRIMER_LEFT_36_PENALTY=0.072951
PRIMER_RIGHT_36_PENALTY=1.227257
PRIMER_LEFT_36_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_36_SEQUENCE=TACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_36=593,20
PRIMER_RIGHT_36=1310,20
PRIMER_LEFT_36_TM=60.073
PRIMER_RIGHT_36_TM=58.773
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=718
PRIMER_PAIR_37_PENALTY=1.307857
PRIMER_LEFT_37_PENALTY=0.090155
PRIMER_RIGHT_37_PENALTY=1.217702
PRIMER_LEFT_37_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_37_SEQUENCE=CTACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_37=447,20
PRIMER_RIGHT_37=1311,21
PRIMER_LEFT_37_TM=59.910
PRIMER_RIGHT_37_TM=59.782
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=11.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=865
PRIMER_PAIR_38_PENALTY=1.312258
PRIMER_LEFT_38_PENALTY=0.934426
PRIMER_RIGHT_38_PENALTY=0.377832
PRIMER_LEFT_38_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_38_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_38=662,20
PRIMER_RIGHT_38=1309,20
PRIMER_LEFT_38_TM=60.934
PRIMER_RIGHT_38_TM=59.622
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=7.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=648
PRIMER_PAIR_39_PENALTY=1.317413
PRIMER_LEFT_39_PENALTY=0.090155
PRIMER_RIGHT_39_PENALTY=1.227257
PRIMER_LEFT_39_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_39_SEQUENCE=TACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_39=447,20
PRIMER_RIGHT_39=1310,20
PRIMER_LEFT_39_TM=59.910
PRIMER_RIGHT_39_TM=58.773
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=11.7000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=864
PRIMER_PAIR_40_PENALTY=1.353449
PRIMER_LEFT_40_PENALTY=0.975617
PRIMER_RIGHT_40_PENALTY=0.377832
PRIMER_LEFT_40_SEQUENCE=CAAATACGAGCCGAGTGAGA
PRIMER_RIGHT_40_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_40=663,20
PRIMER_RIGHT_40=1309,20
PRIMER_LEFT_40_TM=59.024
PRIMER_RIGHT_40_TM=59.622
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=647
PRIMER_PAIR_41_PENALTY=1.364576
PRIMER_LEFT_41_PENALTY=1.216066
PRIMER_RIGHT_41_PENALTY=0.148509
PRIMER_LEFT_41_SEQUENCE=TAAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_41_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_41=453,21
PRIMER_RIGHT_41=1308,20
PRIMER_LEFT_41_TM=60.216
PRIMER_RIGHT_41_TM=60.149
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.7000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=856
PRIMER_PAIR_42_PENALTY=1.400620
PRIMER_LEFT_42_PENALTY=1.022788
PRIMER_RIGHT_42_PENALTY=0.377832
PRIMER_LEFT_42_SEQUENCE=CAAATACGAGCCGAGTGAGAG
PRIMER_RIGHT_42_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_42=663,21
PRIMER_RIGHT_42=1309,20
PRIMER_LEFT_42_TM=60.023
PRIMER_RIGHT_42_TM=59.622
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=647
PRIMER_PAIR_43_PENALTY=1.425367
PRIMER_LEFT_43_PENALTY=0.072951
PRIMER_RIGHT_43_PENALTY=1.352415
PRIMER_LEFT_43_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_43_SEQUENCE=TACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_43=593,20
PRIMER_RIGHT_43=1310,21
PRIMER_LEFT_43_TM=60.073
PRIMER_RIGHT_43_TM=59.648
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=718
PRIMER_PAIR_44_PENALTY=1.431207
PRIMER_LEFT_44_PENALTY=0.213506
PRIMER_RIGHT_44_PENALTY=1.217702
PRIMER_LEFT_44_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_44_SEQUENCE=CTACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_44=454,20
PRIMER_RIGHT_44=1311,21
PRIMER_LEFT_44_TM=60.214
PRIMER_RIGHT_44_TM=59.782
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.7000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=858
PRIMER_PAIR_45_PENALTY=1.432175
PRIMER_LEFT_45_PENALTY=1.054343
PRIMER_RIGHT_45_PENALTY=0.377832
PRIMER_LEFT_45_SEQUENCE=TGGAATGGATTTTGCAGGTAG
PRIMER_RIGHT_45_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_45=341,21
PRIMER_RIGHT_45=1309,20
PRIMER_LEFT_45_TM=59.946
PRIMER_RIGHT_45_TM=59.622
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=969
PRIMER_PAIR_46_PENALTY=1.440763
PRIMER_LEFT_46_PENALTY=0.213506
PRIMER_RIGHT_46_PENALTY=1.227257
PRIMER_LEFT_46_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_46_SEQUENCE=TACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_46=454,20
PRIMER_RIGHT_46=1310,20
PRIMER_LEFT_46_TM=60.214
PRIMER_RIGHT_46_TM=58.773
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=857
PRIMER_PAIR_47_PENALTY=1.442571
PRIMER_LEFT_47_PENALTY=0.090155
PRIMER_RIGHT_47_PENALTY=1.352415
PRIMER_LEFT_47_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_47_SEQUENCE=TACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_47=447,20
PRIMER_RIGHT_47=1310,21
PRIMER_LEFT_47_TM=59.910
PRIMER_RIGHT_47_TM=59.648
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=11.7000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=864
PRIMER_PAIR_48_PENALTY=1.503792
PRIMER_LEFT_48_PENALTY=0.469985
PRIMER_RIGHT_48_PENALTY=1.033807
PRIMER_LEFT_48_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_48_SEQUENCE=CGGCCGTTTAAATCCATACT
PRIMER_LEFT_48=598,20
PRIMER_RIGHT_48=1583,20
PRIMER_LEFT_48_TM=60.470
PRIMER_RIGHT_48_TM=58.966
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=5.3000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=986
PRIMER_PAIR_49_PENALTY=1.504480
PRIMER_LEFT_49_PENALTY=0.934426
PRIMER_RIGHT_49_PENALTY=0.570054
PRIMER_LEFT_49_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_49_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_49=662,20
PRIMER_RIGHT_49=1384,20
PRIMER_LEFT_49_TM=60.934
PRIMER_RIGHT_49_TM=60.570
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=723
=
SEQUENCE_ID=MAGIC.433_1_4274376_4274516_kn-0_ct-1_1401664_249599_TRUE
SEQUENCE_TARGET=950,240
SEQUENCE_TEMPLATE=AATCACGGTAATTAATTAACAATTGTGTAAATAGGGTAAACGTTCCTCGGCAGATCACGTTCTAGAAGCNGNGAATTACATCAAAGACTTACAGANGAAGATCAAAGAGGTCAGCGAAAAAAGAGATAGAATCAAGAGATCTATTACTCATCCATCATCAANAGGAGAATTTTCAATAAGATCATTAGCATCATCAACTTGTNCTTGNGTTGGAGACACGNACATTNCTGTTGTNGTTAGGCCTTGTTTGATCGGACTAGAGATCGTAGTAAGTTGTTGTAACAGANACGAATCTTGTCTATCAAGTGTTCTTCAACTCCTGGCTCAAGAGCAATGCTTTAATATTGTTAGTTGCATCTCAACTAGACTACACCAANGATTCATACACACCATTGNTTCTGAGGTAATTATCTATAATCTATCTTCGATCTGATTTATGAAGAAAGAGGGAGTTAATCTTTATATTTTATGTGNTGAGAGATANATATTAATATTTTGGGAAANTTCTTAAACTTGTGTAGGTCGAGGAGGGAATAGAGGTTTATTTCTCAGAGCTTCAAGAAAAGATAATCAAAATNGGAACCTNANNNNNNNNNNNNNNNNNNNNNNNNTNTATTAAACCTAAAANTTTTANGAATTTACGTCCTAATTAAGATNTNNATCCTCAACCATNNCCACCACCACAAGCATGTNGNGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANATNNNATTTTNNNNNNNTTTTTNNNNNNNNNNNNNNNNNTNNTNNGATNNNTNNTNNTCNTTNNNNNNNNTNTNNANTNCGNTGNNNNNATTNNNNAATTATCGATACANAAACAAAANTATGTTGATNAAAAAGTTGTTANATGTATCATGCATNTNTGNANTNTANNTGGTGTTAGGTNNNGGNANNNTTNNNNNNNNNNNNNNNNNNNNNNNNAAACAATATCTTGAATTATTTTAATTATCNCGAGACAATTNAGANANNNGCAACATTTANNTNTNNNNNNNNNNCCTCAATATNAATANTANTGACAGATATTAANATNCTTAGAAAAACATAAGTANTNNNNNNNNNNATAATTAATTAAGATGCTTCAGTTTCANCNAAACGAATNAAGAAGTAANNGTNANAGAGCGAANANGAGTNNCACAAANAAAACAATGNCGGAATCTTCAGAGCCTCCGNTTGTTCTCCTTCACCGACCACCGTCTCTAACTTTCATGGACGAGATTCTGACTCGTGAATTCCGTACTCTCATCACCGACACTTCNTCATCGGAATCACTCCCNAGCTTCNTCCCCCGTCACGCCTCCTCCGCCAGAGCCTTCGTTATCANCGGCAGACTTCCGGTAACCGANGAACTCCTTTCTCATCTCCCTTCTCTCCAGATNCTCGTCTGCACTAGCGTCGGCATCGATCACATCGATCTCGCCGCATGCAAGCGTCGCGGCATCGTTATCACCAACGCTGGCAACGCGTTTTCCGATGACGTTGCTGATTGTGCNGTCGGTTTGCTNATTAGNGTTCTCCGTCGTATNCCTGCNGCCGATCGTTACGTCCGGNNTGGTAATTGGGCGAAATTTGGGGATTTTCAATTAGGGTCCAAGGTTTGNTCGAAATCACAAACTCTCAATTTCGAATTCTGATCTATAACTNTANTTAGATGATTACAAAGGGGAAATTTAGNTTNAAAGCGNTTTTTTNNATTCTGAGNAAANTTTGGGAATAGCTTTAGANTGTGGGGTTACAGATAAAGNAGAGCTATGTGTTAGTAAAAGTCTTTGTGGNNNNNNNNNGTGATAATATTTATTGTTACAGGTAAGTGGGAAGAGAGTTGGGATAGTTGGATTGGGGAGCATTGGATCATTTGTTGCTAAAAGACTTGAATCATTTGGCTGTGTNATCTCTTACAANTCAAGNAGTCAGAAACAGAGTAGTCCATACCGGTNNTACTCTGANATTCTCTCGTTAGCAGAGAACAACGATGTACTNGTCCTCTGCTGCTCTTTGACAGACGAAACGCACCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.328786
PRIMER_LEFT_0_PENALTY=0.029845
PRIMER_RIGHT_0_PENALTY=0.298942
PRIMER_LEFT_0_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_0_SEQUENCE=TGTCGGTGATGAGAGTACGG
PRIMER_LEFT_0=520,20
PRIMER_RIGHT_0=1378,20
PRIMER_LEFT_0_TM=60.030
PRIMER_RIGHT_0_TM=59.701
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=859
PRIMER_PAIR_1_PENALTY=0.583919
PRIMER_LEFT_1_PENALTY=0.284978
PRIMER_RIGHT_1_PENALTY=0.298942
PRIMER_LEFT_1_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_1_SEQUENCE=TGTCGGTGATGAGAGTACGG
PRIMER_LEFT_1=512,20
PRIMER_RIGHT_1=1378,20
PRIMER_LEFT_1_TM=59.715
PRIMER_RIGHT_1_TM=59.701
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=867
PRIMER_PAIR_2_PENALTY=0.596951
PRIMER_LEFT_2_PENALTY=0.029845
PRIMER_RIGHT_2_PENALTY=0.567107
PRIMER_LEFT_2_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_2_SEQUENCE=GTTAGAGACGGTGGTCGGTG
PRIMER_LEFT_2=520,20
PRIMER_RIGHT_2=1328,20
PRIMER_LEFT_2_TM=60.030
PRIMER_RIGHT_2_TM=60.567
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=809
PRIMER_PAIR_3_PENALTY=0.788999
PRIMER_LEFT_3_PENALTY=0.029845
PRIMER_RIGHT_3_PENALTY=0.759154
PRIMER_LEFT_3_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_3_SEQUENCE=TCGGTGATGAGAGTACGGAA
PRIMER_LEFT_3=520,20
PRIMER_RIGHT_3=1376,20
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=59.241
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=857
PRIMER_PAIR_4_PENALTY=0.822081
PRIMER_LEFT_4_PENALTY=0.029845
PRIMER_RIGHT_4_PENALTY=0.792236
PRIMER_LEFT_4_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_4_SEQUENCE=AAGTTAGAGACGGTGGTCGG
PRIMER_LEFT_4=520,20
PRIMER_RIGHT_4=1330,20
PRIMER_LEFT_4_TM=60.030
PRIMER_RIGHT_4_TM=59.208
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=811
PRIMER_PAIR_5_PENALTY=0.852084
PRIMER_LEFT_5_PENALTY=0.284978
PRIMER_RIGHT_5_PENALTY=0.567107
PRIMER_LEFT_5_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_5_SEQUENCE=GTTAGAGACGGTGGTCGGTG
PRIMER_LEFT_5=512,20
PRIMER_RIGHT_5=1328,20
PRIMER_LEFT_5_TM=59.715
PRIMER_RIGHT_5_TM=60.567
PRIMER_LEFT_5_GC_PERCENT=60.000
PRIMER_RIGHT_5_GC_PERCENT=60.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=817
PRIMER_PAIR_6_PENALTY=0.945276
PRIMER_LEFT_6_PENALTY=0.646334
PRIMER_RIGHT_6_PENALTY=0.298942
PRIMER_LEFT_6_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_6_SEQUENCE=TGTCGGTGATGAGAGTACGG
PRIMER_LEFT_6=514,20
PRIMER_RIGHT_6=1378,20
PRIMER_LEFT_6_TM=60.646
PRIMER_RIGHT_6_TM=59.701
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=8.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=865
PRIMER_PAIR_7_PENALTY=0.945276
PRIMER_LEFT_7_PENALTY=0.646334
PRIMER_RIGHT_7_PENALTY=0.298942
PRIMER_LEFT_7_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_7_SEQUENCE=TGTCGGTGATGAGAGTACGG
PRIMER_LEFT_7=513,20
PRIMER_RIGHT_7=1378,20
PRIMER_LEFT_7_TM=60.646
PRIMER_RIGHT_7_TM=59.701
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=8.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=866
PRIMER_PAIR_8_PENALTY=1.044132
PRIMER_LEFT_8_PENALTY=0.284978
PRIMER_RIGHT_8_PENALTY=0.759154
PRIMER_LEFT_8_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_8_SEQUENCE=TCGGTGATGAGAGTACGGAA
PRIMER_LEFT_8=512,20
PRIMER_RIGHT_8=1376,20
PRIMER_LEFT_8_TM=59.715
PRIMER_RIGHT_8_TM=59.241
PRIMER_LEFT_8_GC_PERCENT=60.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=10.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=865
PRIMER_PAIR_9_PENALTY=1.075277
PRIMER_LEFT_9_PENALTY=0.029845
PRIMER_RIGHT_9_PENALTY=1.045432
PRIMER_LEFT_9_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_9_SEQUENCE=AAGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_9=520,20
PRIMER_RIGHT_9=1330,21
PRIMER_LEFT_9_TM=60.030
PRIMER_RIGHT_9_TM=60.045
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=811
PRIMER_PAIR_10_PENALTY=1.077214
PRIMER_LEFT_10_PENALTY=0.284978
PRIMER_RIGHT_10_PENALTY=0.792236
PRIMER_LEFT_10_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_10_SEQUENCE=AAGTTAGAGACGGTGGTCGG
PRIMER_LEFT_10=512,20
PRIMER_RIGHT_10=1330,20
PRIMER_LEFT_10_TM=59.715
PRIMER_RIGHT_10_TM=59.208
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=819
PRIMER_PAIR_11_PENALTY=1.142796
PRIMER_LEFT_11_PENALTY=0.029845
PRIMER_RIGHT_11_PENALTY=1.112951
PRIMER_LEFT_11_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_11_SEQUENCE=GTGGTCGGTGAAGGAGAACA
PRIMER_LEFT_11=520,20
PRIMER_RIGHT_11=1318,20
PRIMER_LEFT_11_TM=60.030
PRIMER_RIGHT_11_TM=61.113
PRIMER_LEFT_11_GC_PERCENT=60.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=799
PRIMER_PAIR_12_PENALTY=1.156128
PRIMER_LEFT_12_PENALTY=0.029845
PRIMER_RIGHT_12_PENALTY=1.126283
PRIMER_LEFT_12_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_12_SEQUENCE=GTCGGTGATGAGAGTACGGAA
PRIMER_LEFT_12=520,20
PRIMER_RIGHT_12=1377,21
PRIMER_LEFT_12_TM=60.030
PRIMER_RIGHT_12_TM=60.126
PRIMER_LEFT_12_GC_PERCENT=60.000
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=10.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=858
PRIMER_PAIR_13_PENALTY=1.213441
PRIMER_LEFT_13_PENALTY=0.646334
PRIMER_RIGHT_13_PENALTY=0.567107
PRIMER_LEFT_13_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_13_SEQUENCE=GTTAGAGACGGTGGTCGGTG
PRIMER_LEFT_13=514,20
PRIMER_RIGHT_13=1328,20
PRIMER_LEFT_13_TM=60.646
PRIMER_RIGHT_13_TM=60.567
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=60.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=815
PRIMER_PAIR_14_PENALTY=1.213441
PRIMER_LEFT_14_PENALTY=0.646334
PRIMER_RIGHT_14_PENALTY=0.567107
PRIMER_LEFT_14_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_14_SEQUENCE=GTTAGAGACGGTGGTCGGTG
PRIMER_LEFT_14=513,20
PRIMER_RIGHT_14=1328,20
PRIMER_LEFT_14_TM=60.646
PRIMER_RIGHT_14_TM=60.567
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=60.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=816
PRIMER_PAIR_15_PENALTY=1.330410
PRIMER_LEFT_15_PENALTY=0.284978
PRIMER_RIGHT_15_PENALTY=1.045432
PRIMER_LEFT_15_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_15_SEQUENCE=AAGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_15=512,20
PRIMER_RIGHT_15=1330,21
PRIMER_LEFT_15_TM=59.715
PRIMER_RIGHT_15_TM=60.045
PRIMER_LEFT_15_GC_PERCENT=60.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=819
PRIMER_PAIR_16_PENALTY=1.359028
PRIMER_LEFT_16_PENALTY=0.029845
PRIMER_RIGHT_16_PENALTY=1.329183
PRIMER_LEFT_16_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_16_SEQUENCE=AGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_16=520,20
PRIMER_RIGHT_16=1329,20
PRIMER_LEFT_16_TM=60.030
PRIMER_RIGHT_16_TM=58.671
PRIMER_LEFT_16_GC_PERCENT=60.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=810
PRIMER_PAIR_17_PENALTY=1.397929
PRIMER_LEFT_17_PENALTY=0.284978
PRIMER_RIGHT_17_PENALTY=1.112951
PRIMER_LEFT_17_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_17_SEQUENCE=GTGGTCGGTGAAGGAGAACA
PRIMER_LEFT_17=512,20
PRIMER_RIGHT_17=1318,20
PRIMER_LEFT_17_TM=59.715
PRIMER_RIGHT_17_TM=61.113
PRIMER_LEFT_17_GC_PERCENT=60.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=807
PRIMER_PAIR_18_PENALTY=1.405489
PRIMER_LEFT_18_PENALTY=0.646334
PRIMER_RIGHT_18_PENALTY=0.759154
PRIMER_LEFT_18_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_18_SEQUENCE=TCGGTGATGAGAGTACGGAA
PRIMER_LEFT_18=514,20
PRIMER_RIGHT_18=1376,20
PRIMER_LEFT_18_TM=60.646
PRIMER_RIGHT_18_TM=59.241
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=10.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=863
PRIMER_PAIR_19_PENALTY=1.405489
PRIMER_LEFT_19_PENALTY=0.646334
PRIMER_RIGHT_19_PENALTY=0.759154
PRIMER_LEFT_19_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_19_SEQUENCE=TCGGTGATGAGAGTACGGAA
PRIMER_LEFT_19=513,20
PRIMER_RIGHT_19=1376,20
PRIMER_LEFT_19_TM=60.646
PRIMER_RIGHT_19_TM=59.241
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=10.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=864
PRIMER_PAIR_20_PENALTY=1.411261
PRIMER_LEFT_20_PENALTY=0.284978
PRIMER_RIGHT_20_PENALTY=1.126283
PRIMER_LEFT_20_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_20_SEQUENCE=GTCGGTGATGAGAGTACGGAA
PRIMER_LEFT_20=512,20
PRIMER_RIGHT_20=1377,21
PRIMER_LEFT_20_TM=59.715
PRIMER_RIGHT_20_TM=60.126
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=10.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=866
PRIMER_PAIR_21_PENALTY=1.431487
PRIMER_LEFT_21_PENALTY=0.029845
PRIMER_RIGHT_21_PENALTY=1.401642
PRIMER_LEFT_21_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_21_SEQUENCE=TCACGAGTCAGAATCTCGTCC
PRIMER_LEFT_21=520,20
PRIMER_RIGHT_21=1355,21
PRIMER_LEFT_21_TM=60.030
PRIMER_RIGHT_21_TM=60.402
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=836
PRIMER_PAIR_22_PENALTY=1.438570
PRIMER_LEFT_22_PENALTY=0.646334
PRIMER_RIGHT_22_PENALTY=0.792236
PRIMER_LEFT_22_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_22_SEQUENCE=AAGTTAGAGACGGTGGTCGG
PRIMER_LEFT_22=514,20
PRIMER_RIGHT_22=1330,20
PRIMER_LEFT_22_TM=60.646
PRIMER_RIGHT_22_TM=59.208
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=9.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=817
PRIMER_PAIR_23_PENALTY=1.438570
PRIMER_LEFT_23_PENALTY=0.646334
PRIMER_RIGHT_23_PENALTY=0.792236
PRIMER_LEFT_23_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_23_SEQUENCE=AAGTTAGAGACGGTGGTCGG
PRIMER_LEFT_23=513,20
PRIMER_RIGHT_23=1330,20
PRIMER_LEFT_23_TM=60.646
PRIMER_RIGHT_23_TM=59.208
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=818
PRIMER_PAIR_24_PENALTY=1.563794
PRIMER_LEFT_24_PENALTY=0.029845
PRIMER_RIGHT_24_PENALTY=1.533949
PRIMER_LEFT_24_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_24_SEQUENCE=AAAGTTAGAGACGGTGGTCGG
PRIMER_LEFT_24=520,20
PRIMER_RIGHT_24=1331,21
PRIMER_LEFT_24_TM=60.030
PRIMER_RIGHT_24_TM=60.534
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=812
PRIMER_PAIR_25_PENALTY=1.612751
PRIMER_LEFT_25_PENALTY=0.029845
PRIMER_RIGHT_25_PENALTY=1.582907
PRIMER_LEFT_25_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_25_SEQUENCE=GTGTCGGTGATGAGAGTACGG
PRIMER_LEFT_25=520,20
PRIMER_RIGHT_25=1379,21
PRIMER_LEFT_25_TM=60.030
PRIMER_RIGHT_25_TM=60.583
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=57.143
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=8.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=860
PRIMER_PAIR_26_PENALTY=1.614161
PRIMER_LEFT_26_PENALTY=0.284978
PRIMER_RIGHT_26_PENALTY=1.329183
PRIMER_LEFT_26_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_26_SEQUENCE=AGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_26=512,20
PRIMER_RIGHT_26=1329,20
PRIMER_LEFT_26_TM=59.715
PRIMER_RIGHT_26_TM=58.671
PRIMER_LEFT_26_GC_PERCENT=60.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=818
PRIMER_PAIR_27_PENALTY=1.651246
PRIMER_LEFT_27_PENALTY=0.029845
PRIMER_RIGHT_27_PENALTY=1.621401
PRIMER_LEFT_27_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_27_SEQUENCE=ACGAGTCAGAATCTCGTCCA
PRIMER_LEFT_27=520,20
PRIMER_RIGHT_27=1353,20
PRIMER_LEFT_27_TM=60.030
PRIMER_RIGHT_27_TM=58.379
PRIMER_LEFT_27_GC_PERCENT=60.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=834
PRIMER_PAIR_28_PENALTY=1.651246
PRIMER_LEFT_28_PENALTY=0.029845
PRIMER_RIGHT_28_PENALTY=1.621401
PRIMER_LEFT_28_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_28_SEQUENCE=CACGAGTCAGAATCTCGTCC
PRIMER_LEFT_28=520,20
PRIMER_RIGHT_28=1354,20
PRIMER_LEFT_28_TM=60.030
PRIMER_RIGHT_28_TM=58.379
PRIMER_LEFT_28_GC_PERCENT=60.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=835
PRIMER_PAIR_29_PENALTY=1.665469
PRIMER_LEFT_29_PENALTY=0.029845
PRIMER_RIGHT_29_PENALTY=1.635624
PRIMER_LEFT_29_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_29_SEQUENCE=TGGTCGGTGAAGGAGAACAA
PRIMER_LEFT_29=520,20
PRIMER_RIGHT_29=1317,20
PRIMER_LEFT_29_TM=60.030
PRIMER_RIGHT_29_TM=61.636
PRIMER_LEFT_29_GC_PERCENT=60.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=798
PRIMER_PAIR_30_PENALTY=1.684748
PRIMER_LEFT_30_PENALTY=0.029845
PRIMER_RIGHT_30_PENALTY=1.654904
PRIMER_LEFT_30_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_30_SEQUENCE=TAGAGACGGTGGTCGGTGAA
PRIMER_LEFT_30=520,20
PRIMER_RIGHT_30=1326,20
PRIMER_LEFT_30_TM=60.030
PRIMER_RIGHT_30_TM=61.655
PRIMER_LEFT_30_GC_PERCENT=60.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=807
PRIMER_PAIR_31_PENALTY=1.684748
PRIMER_LEFT_31_PENALTY=0.029845
PRIMER_RIGHT_31_PENALTY=1.654904
PRIMER_LEFT_31_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_31_SEQUENCE=TTAGAGACGGTGGTCGGTGA
PRIMER_LEFT_31=520,20
PRIMER_RIGHT_31=1327,20
PRIMER_LEFT_31_TM=60.030
PRIMER_RIGHT_31_TM=61.655
PRIMER_LEFT_31_GC_PERCENT=60.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=808
PRIMER_PAIR_32_PENALTY=1.686620
PRIMER_LEFT_32_PENALTY=0.284978
PRIMER_RIGHT_32_PENALTY=1.401642
PRIMER_LEFT_32_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_32_SEQUENCE=TCACGAGTCAGAATCTCGTCC
PRIMER_LEFT_32=512,20
PRIMER_RIGHT_32=1355,21
PRIMER_LEFT_32_TM=59.715
PRIMER_RIGHT_32_TM=60.402
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=844
PRIMER_PAIR_33_PENALTY=1.691767
PRIMER_LEFT_33_PENALTY=0.646334
PRIMER_RIGHT_33_PENALTY=1.045432
PRIMER_LEFT_33_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_33_SEQUENCE=AAGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_33=514,20
PRIMER_RIGHT_33=1330,21
PRIMER_LEFT_33_TM=60.646
PRIMER_RIGHT_33_TM=60.045
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=817
PRIMER_PAIR_34_PENALTY=1.691767
PRIMER_LEFT_34_PENALTY=0.646334
PRIMER_RIGHT_34_PENALTY=1.045432
PRIMER_LEFT_34_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_34_SEQUENCE=AAGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_34=513,20
PRIMER_RIGHT_34=1330,21
PRIMER_LEFT_34_TM=60.646
PRIMER_RIGHT_34_TM=60.045
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=818
PRIMER_PAIR_35_PENALTY=1.759286
PRIMER_LEFT_35_PENALTY=0.646334
PRIMER_RIGHT_35_PENALTY=1.112951
PRIMER_LEFT_35_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_35_SEQUENCE=GTGGTCGGTGAAGGAGAACA
PRIMER_LEFT_35=514,20
PRIMER_RIGHT_35=1318,20
PRIMER_LEFT_35_TM=60.646
PRIMER_RIGHT_35_TM=61.113
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=805
PRIMER_PAIR_36_PENALTY=1.759286
PRIMER_LEFT_36_PENALTY=0.646334
PRIMER_RIGHT_36_PENALTY=1.112951
PRIMER_LEFT_36_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_36_SEQUENCE=GTGGTCGGTGAAGGAGAACA
PRIMER_LEFT_36=513,20
PRIMER_RIGHT_36=1318,20
PRIMER_LEFT_36_TM=60.646
PRIMER_RIGHT_36_TM=61.113
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=806
PRIMER_PAIR_37_PENALTY=1.772618
PRIMER_LEFT_37_PENALTY=0.646334
PRIMER_RIGHT_37_PENALTY=1.126283
PRIMER_LEFT_37_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_37_SEQUENCE=GTCGGTGATGAGAGTACGGAA
PRIMER_LEFT_37=514,20
PRIMER_RIGHT_37=1377,21
PRIMER_LEFT_37_TM=60.646
PRIMER_RIGHT_37_TM=60.126
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=10.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=864
PRIMER_PAIR_38_PENALTY=1.772618
PRIMER_LEFT_38_PENALTY=0.646334
PRIMER_RIGHT_38_PENALTY=1.126283
PRIMER_LEFT_38_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_38_SEQUENCE=GTCGGTGATGAGAGTACGGAA
PRIMER_LEFT_38=513,20
PRIMER_RIGHT_38=1377,21
PRIMER_LEFT_38_TM=60.646
PRIMER_RIGHT_38_TM=60.126
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=10.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=865
PRIMER_PAIR_39_PENALTY=1.818927
PRIMER_LEFT_39_PENALTY=0.284978
PRIMER_RIGHT_39_PENALTY=1.533949
PRIMER_LEFT_39_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_39_SEQUENCE=AAAGTTAGAGACGGTGGTCGG
PRIMER_LEFT_39=512,20
PRIMER_RIGHT_39=1331,21
PRIMER_LEFT_39_TM=59.715
PRIMER_RIGHT_39_TM=60.534
PRIMER_LEFT_39_GC_PERCENT=60.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=820
PRIMER_PAIR_40_PENALTY=1.848447
PRIMER_LEFT_40_PENALTY=1.549505
PRIMER_RIGHT_40_PENALTY=0.298942
PRIMER_LEFT_40_SEQUENCE=ACTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_40_SEQUENCE=TGTCGGTGATGAGAGTACGG
PRIMER_LEFT_40=511,21
PRIMER_RIGHT_40=1378,20
PRIMER_LEFT_40_TM=60.550
PRIMER_RIGHT_40_TM=59.701
PRIMER_LEFT_40_GC_PERCENT=57.143
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=8.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=868
PRIMER_PAIR_41_PENALTY=1.867884
PRIMER_LEFT_41_PENALTY=0.284978
PRIMER_RIGHT_41_PENALTY=1.582907
PRIMER_LEFT_41_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_41_SEQUENCE=GTGTCGGTGATGAGAGTACGG
PRIMER_LEFT_41=512,20
PRIMER_RIGHT_41=1379,21
PRIMER_LEFT_41_TM=59.715
PRIMER_RIGHT_41_TM=60.583
PRIMER_LEFT_41_GC_PERCENT=60.000
PRIMER_RIGHT_41_GC_PERCENT=57.143
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=8.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=868
PRIMER_PAIR_42_PENALTY=1.906379
PRIMER_LEFT_42_PENALTY=0.284978
PRIMER_RIGHT_42_PENALTY=1.621401
PRIMER_LEFT_42_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_42_SEQUENCE=ACGAGTCAGAATCTCGTCCA
PRIMER_LEFT_42=512,20
PRIMER_RIGHT_42=1353,20
PRIMER_LEFT_42_TM=59.715
PRIMER_RIGHT_42_TM=58.379
PRIMER_LEFT_42_GC_PERCENT=60.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.4000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=842
PRIMER_PAIR_43_PENALTY=1.906379
PRIMER_LEFT_43_PENALTY=0.284978
PRIMER_RIGHT_43_PENALTY=1.621401
PRIMER_LEFT_43_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_43_SEQUENCE=CACGAGTCAGAATCTCGTCC
PRIMER_LEFT_43=512,20
PRIMER_RIGHT_43=1354,20
PRIMER_LEFT_43_TM=59.715
PRIMER_RIGHT_43_TM=58.379
PRIMER_LEFT_43_GC_PERCENT=60.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=843
PRIMER_PAIR_44_PENALTY=1.916377
PRIMER_LEFT_44_PENALTY=0.029845
PRIMER_RIGHT_44_PENALTY=1.886532
PRIMER_LEFT_44_SEQUENCE=GGTCGAGGAGGGAATAGAGG
PRIMER_RIGHT_44_SEQUENCE=GGTGGTCGGTGAAGGAGAAC
PRIMER_LEFT_44=520,20
PRIMER_RIGHT_44=1319,20
PRIMER_LEFT_44_TM=60.030
PRIMER_RIGHT_44_TM=61.887
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=60.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=800
PRIMER_PAIR_45_PENALTY=1.920602
PRIMER_LEFT_45_PENALTY=0.284978
PRIMER_RIGHT_45_PENALTY=1.635624
PRIMER_LEFT_45_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_45_SEQUENCE=TGGTCGGTGAAGGAGAACAA
PRIMER_LEFT_45=512,20
PRIMER_RIGHT_45=1317,20
PRIMER_LEFT_45_TM=59.715
PRIMER_RIGHT_45_TM=61.636
PRIMER_LEFT_45_GC_PERCENT=60.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=806
PRIMER_PAIR_46_PENALTY=1.939881
PRIMER_LEFT_46_PENALTY=0.284978
PRIMER_RIGHT_46_PENALTY=1.654904
PRIMER_LEFT_46_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_46_SEQUENCE=TAGAGACGGTGGTCGGTGAA
PRIMER_LEFT_46=512,20
PRIMER_RIGHT_46=1326,20
PRIMER_LEFT_46_TM=59.715
PRIMER_RIGHT_46_TM=61.655
PRIMER_LEFT_46_GC_PERCENT=60.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=815
PRIMER_PAIR_47_PENALTY=1.939881
PRIMER_LEFT_47_PENALTY=0.284978
PRIMER_RIGHT_47_PENALTY=1.654904
PRIMER_LEFT_47_SEQUENCE=CTTGTGTAGGTCGAGGAGGG
PRIMER_RIGHT_47_SEQUENCE=TTAGAGACGGTGGTCGGTGA
PRIMER_LEFT_47=512,20
PRIMER_RIGHT_47=1327,20
PRIMER_LEFT_47_TM=59.715
PRIMER_RIGHT_47_TM=61.655
PRIMER_LEFT_47_GC_PERCENT=60.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=816
PRIMER_PAIR_48_PENALTY=1.975517
PRIMER_LEFT_48_PENALTY=0.646334
PRIMER_RIGHT_48_PENALTY=1.329183
PRIMER_LEFT_48_SEQUENCE=TGTGTAGGTCGAGGAGGGAA
PRIMER_RIGHT_48_SEQUENCE=AGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_48=514,20
PRIMER_RIGHT_48=1329,20
PRIMER_LEFT_48_TM=60.646
PRIMER_RIGHT_48_TM=58.671
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=816
PRIMER_PAIR_49_PENALTY=1.975517
PRIMER_LEFT_49_PENALTY=0.646334
PRIMER_RIGHT_49_PENALTY=1.329183
PRIMER_LEFT_49_SEQUENCE=TTGTGTAGGTCGAGGAGGGA
PRIMER_RIGHT_49_SEQUENCE=AGTTAGAGACGGTGGTCGGT
PRIMER_LEFT_49=513,20
PRIMER_RIGHT_49=1329,20
PRIMER_LEFT_49_TM=60.646
PRIMER_RIGHT_49_TM=58.671
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=817
=
SEQUENCE_ID=MAGIC.338_1_13086820_13086880_edi-0_can-0_24375_63887_TRUE
SEQUENCE_TARGET=950,160
SEQUENCE_TEMPLATE=NCAGTTTNCATAACCTCTTATCCAAAGATCGGTATACCAAAATATGTNATNTCAACAATTATTATACAATTTTCTNCCTTTCCTCAACTTCTCTCGNGACCATGAGAANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGAATANGAATNCNCNAGCACCGTGATCNGATCTATNATATNGGATCTATGGTACATCTCATNTTAGNTTTTTTNTTTTGCTTCCTCTTTAANTTTTTAAGGATNCCTTATAAACNGGNTTTCTCATATATNGTNNCNNTTTGNAGAAATCTCCAAGGACATATTCCCTTTTTCATTTAGNTTCCCCNTTCCANTTAGCTATGATGTGAATTTGTNTGTATCCCATNACTATTAGGATNTNNTTGGCATCAGGGGTNGATCACCGGTGGTGGTGATGGTATGATGAATTCAATCAAAAATGTACAAGNGCTTCTCGCTGCCTCTCAATCCGAATGTCATNTAAGNATTTTTNCTCCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNATGTCTCCACACGNCATCANCAAAGGGACAATNAAATAGAATATNATTCACCGNTTCGTNTGCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_13141911_13142021_sf-2_ler-0_14377_7664_TRUE
SEQUENCE_TARGET=950,210
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_5198629_5198712_po-0_can-0_12789_12718_TRUE
SEQUENCE_TARGET=950,183
SEQUENCE_TEMPLATE=GCCATTTTCATATCANCNCTGTAAACTTGNCACCAATGCATTCAGCACATCATCTTTGAAACTTGTAAGAGGAGAAGNAGTGGTAATCAATTGTATGTTGCATCTTCCTCGGTTTAGTCACCAAACACCCANTTCNGTCATTTCCTTTTTGTCGGAAGCTAAAACTCTAAACCCNAAACTTGTTACNCTTGTTCACGAAGAAGTTGGTCTGATGGGNAACCAAGGTTTTCTATATNGTTTTATGGACTTGCTACATCAGTTCTCNGCCATATTCGACTCTCTAGAGGCAGGCCTTTCAATNNCNAACCCGGCCCGTGGCTTTGTTGAGCGTGTTTTTATCGGTCCTTGGGTTGCNNATTGGTTGANGCGTATCACCGCTNATGATGCAGAAGTCGAGAGCTNTGCTTCGTGGCNACAGTGGTTAGAGACTAATGGGTTCAAACCCTTGGAAGTGAGCTTTNCCAACCGTTGTCAAGCAAAACTCCTTTTGAGTTTGTTTAANGATNGATTTAGAGTGGAAGAATTGGGCCAANACGGNCTCGTTTTGGGATGGAAATCTCGGCGTCTTGTNTNNGCCTCCTTTTGGGCCTCGTGCNAGACGAATCAGTAAATTCTCGTTTNTGCATTCTCGAGTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTATGTGAATATCGAACGTGTAATTTCTCAAAAANGACAGTTTAGTTTAATATGANAAGTNATTGAGCCACGACATTGCATTGTATGTTGAATCTTTAATCNTNTATATAAATTANAATGANNAACTCGACANTCGTTTTACACAAGACTATGTTTGNANATACGTGTNAATATGTTGTAGTAATTTTGGTAGCGGACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNGCCATACTTGTAGCCGCAAACANCATAAATAATATTTTTGGTCAAAGTTTCATCATTGGAATTGGTAACTCTCTTANTTGAANTGNTGGAANTAGGGTTCCAACCNTCCTCTTTTCATAAAGCTTGTTTAATTTATTTATTTTANNTGNTTGCAGNCTANAATANNNTNAGCTGCATAGAAGNGGANACGGAAANGCTCNGAATTNCTCGAAATCGTCTCAGAATCGCGANTNCTAAAATGATGNGTTTGGANACGTGGANGAANCGGACAATTCAGCTTTNGAANCGTCGGTGGTTGAGATCGANAATGTCTCTACCGTAAATCNAAGATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.089485
PRIMER_LEFT_0_PENALTY=0.205314
PRIMER_RIGHT_0_PENALTY=0.884171
PRIMER_LEFT_0_SEQUENCE=TTTTATCGGTCCTTGGGTTG
PRIMER_RIGHT_0_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_0=333,20
PRIMER_RIGHT_0=1153,20
PRIMER_LEFT_0_TM=59.795
PRIMER_RIGHT_0_TM=60.884
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=821
PRIMER_PAIR_1_PENALTY=1.089485
PRIMER_LEFT_1_PENALTY=0.205314
PRIMER_RIGHT_1_PENALTY=0.884171
PRIMER_LEFT_1_SEQUENCE=TGTTTTTATCGGTCCTTGGG
PRIMER_RIGHT_1_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_1=330,20
PRIMER_RIGHT_1=1153,20
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=60.884
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=824
PRIMER_PAIR_2_PENALTY=1.175840
PRIMER_LEFT_2_PENALTY=0.291669
PRIMER_RIGHT_2_PENALTY=0.884171
PRIMER_LEFT_2_SEQUENCE=GGCTTTGTTGAGCGTGTTTT
PRIMER_RIGHT_2_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_2=316,20
PRIMER_RIGHT_2=1153,20
PRIMER_LEFT_2_TM=60.292
PRIMER_RIGHT_2_TM=60.884
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=838
PRIMER_PAIR_3_PENALTY=1.552486
PRIMER_LEFT_3_PENALTY=0.668315
PRIMER_RIGHT_3_PENALTY=0.884171
PRIMER_LEFT_3_SEQUENCE=CCGTTGTCAAGCAAAACTCC
PRIMER_RIGHT_3_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_3=465,20
PRIMER_RIGHT_3=1153,20
PRIMER_LEFT_3_TM=60.668
PRIMER_RIGHT_3_TM=60.884
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=689
PRIMER_PAIR_4_PENALTY=1.793406
PRIMER_LEFT_4_PENALTY=0.909234
PRIMER_RIGHT_4_PENALTY=0.884171
PRIMER_LEFT_4_SEQUENCE=GCGTGTTTTTATCGGTCCTT
PRIMER_RIGHT_4_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_4=327,20
PRIMER_RIGHT_4=1153,20
PRIMER_LEFT_4_TM=59.091
PRIMER_RIGHT_4_TM=60.884
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=827
PRIMER_PAIR_5_PENALTY=1.898392
PRIMER_LEFT_5_PENALTY=1.014220
PRIMER_RIGHT_5_PENALTY=0.884171
PRIMER_LEFT_5_SEQUENCE=AGCGTGTTTTTATCGGTCCTT
PRIMER_RIGHT_5_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_5=326,21
PRIMER_RIGHT_5=1153,20
PRIMER_LEFT_5_TM=60.014
PRIMER_RIGHT_5_TM=60.884
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=828
PRIMER_PAIR_6_PENALTY=2.008096
PRIMER_LEFT_6_PENALTY=1.123924
PRIMER_RIGHT_6_PENALTY=0.884171
PRIMER_LEFT_6_SEQUENCE=TTGAGCGTGTTTTTATCGGTC
PRIMER_RIGHT_6_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_6=323,21
PRIMER_RIGHT_6=1153,20
PRIMER_LEFT_6_TM=60.124
PRIMER_RIGHT_6_TM=60.884
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=831
PRIMER_PAIR_7_PENALTY=2.111073
PRIMER_LEFT_7_PENALTY=1.226901
PRIMER_RIGHT_7_PENALTY=0.884171
PRIMER_LEFT_7_SEQUENCE=TGAGCGTGTTTTTATCGGTC
PRIMER_RIGHT_7_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_7=324,20
PRIMER_RIGHT_7=1153,20
PRIMER_LEFT_7_TM=58.773
PRIMER_RIGHT_7_TM=60.884
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=830
PRIMER_PAIR_8_PENALTY=2.159508
PRIMER_LEFT_8_PENALTY=1.275336
PRIMER_RIGHT_8_PENALTY=0.884171
PRIMER_LEFT_8_SEQUENCE=GTGGCTTTGTTGAGCGTGTT
PRIMER_RIGHT_8_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_8=314,20
PRIMER_RIGHT_8=1153,20
PRIMER_LEFT_8_TM=61.275
PRIMER_RIGHT_8_TM=60.884
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=840
PRIMER_PAIR_9_PENALTY=2.163925
PRIMER_LEFT_9_PENALTY=1.279754
PRIMER_RIGHT_9_PENALTY=0.884171
PRIMER_LEFT_9_SEQUENCE=AGCGTGTTTTTATCGGTCCT
PRIMER_RIGHT_9_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_9=326,20
PRIMER_RIGHT_9=1153,20
PRIMER_LEFT_9_TM=58.720
PRIMER_RIGHT_9_TM=60.884
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=828
PRIMER_PAIR_10_PENALTY=2.375256
PRIMER_LEFT_10_PENALTY=1.491085
PRIMER_RIGHT_10_PENALTY=0.884171
PRIMER_LEFT_10_SEQUENCE=GATGGAAATCTCGGCGTCTT
PRIMER_RIGHT_10_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_10=548,20
PRIMER_RIGHT_10=1153,20
PRIMER_LEFT_10_TM=61.491
PRIMER_RIGHT_10_TM=60.884
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=606
PRIMER_PAIR_11_PENALTY=2.381047
PRIMER_LEFT_11_PENALTY=1.496875
PRIMER_RIGHT_11_PENALTY=0.884171
PRIMER_LEFT_11_SEQUENCE=GAGCGTGTTTTTATCGGTCCT
PRIMER_RIGHT_11_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_11=325,21
PRIMER_RIGHT_11=1153,20
PRIMER_LEFT_11_TM=60.497
PRIMER_RIGHT_11_TM=60.884
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=829
PRIMER_PAIR_12_PENALTY=2.462729
PRIMER_LEFT_12_PENALTY=1.578557
PRIMER_RIGHT_12_PENALTY=0.884171
PRIMER_LEFT_12_SEQUENCE=TGTTTTTATCGGTCCTTGGGT
PRIMER_RIGHT_12_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_12=330,21
PRIMER_RIGHT_12=1153,20
PRIMER_LEFT_12_TM=60.579
PRIMER_RIGHT_12_TM=60.884
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=824
PRIMER_PAIR_13_PENALTY=2.462729
PRIMER_LEFT_13_PENALTY=1.578557
PRIMER_RIGHT_13_PENALTY=0.884171
PRIMER_LEFT_13_SEQUENCE=GTGTTTTTATCGGTCCTTGGG
PRIMER_RIGHT_13_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_13=329,21
PRIMER_RIGHT_13=1153,20
PRIMER_LEFT_13_TM=60.579
PRIMER_RIGHT_13_TM=60.884
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=825
PRIMER_PAIR_14_PENALTY=2.467051
PRIMER_LEFT_14_PENALTY=1.582879
PRIMER_RIGHT_14_PENALTY=0.884171
PRIMER_LEFT_14_SEQUENCE=TTTTTATCGGTCCTTGGGTT
PRIMER_RIGHT_14_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_14=332,20
PRIMER_RIGHT_14=1153,20
PRIMER_LEFT_14_TM=58.417
PRIMER_RIGHT_14_TM=60.884
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=822
PRIMER_PAIR_15_PENALTY=2.590453
PRIMER_LEFT_15_PENALTY=1.706282
PRIMER_RIGHT_15_PENALTY=0.884171
PRIMER_LEFT_15_SEQUENCE=GTTGAGCGTGTTTTTATCGG
PRIMER_RIGHT_15_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_15=322,20
PRIMER_RIGHT_15=1153,20
PRIMER_LEFT_15_TM=58.294
PRIMER_RIGHT_15_TM=60.884
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.8000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=832
PRIMER_PAIR_16_PENALTY=2.597786
PRIMER_LEFT_16_PENALTY=1.713615
PRIMER_RIGHT_16_PENALTY=0.884171
PRIMER_LEFT_16_SEQUENCE=CGAAGAAGTTGGTCTGATGG
PRIMER_RIGHT_16_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_16=195,20
PRIMER_RIGHT_16=1153,20
PRIMER_LEFT_16_TM=58.286
PRIMER_RIGHT_16_TM=60.884
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=959
PRIMER_PAIR_17_PENALTY=2.608332
PRIMER_LEFT_17_PENALTY=1.724161
PRIMER_RIGHT_17_PENALTY=0.884171
PRIMER_LEFT_17_SEQUENCE=AACCGTTGTCAAGCAAAACTC
PRIMER_RIGHT_17_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_17=463,21
PRIMER_RIGHT_17=1153,20
PRIMER_LEFT_17_TM=59.276
PRIMER_RIGHT_17_TM=60.884
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=691
PRIMER_PAIR_18_PENALTY=2.651562
PRIMER_LEFT_18_PENALTY=1.767391
PRIMER_RIGHT_18_PENALTY=0.884171
PRIMER_LEFT_18_SEQUENCE=GTTTTTATCGGTCCTTGGGTT
PRIMER_RIGHT_18_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_18=331,21
PRIMER_RIGHT_18=1153,20
PRIMER_LEFT_18_TM=59.233
PRIMER_RIGHT_18_TM=60.884
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=823
PRIMER_PAIR_19_PENALTY=2.654824
PRIMER_LEFT_19_PENALTY=1.770653
PRIMER_RIGHT_19_PENALTY=0.884171
PRIMER_LEFT_19_SEQUENCE=TGGCTTTGTTGAGCGTGTTT
PRIMER_RIGHT_19_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_19=315,20
PRIMER_RIGHT_19=1153,20
PRIMER_LEFT_19_TM=61.771
PRIMER_RIGHT_19_TM=60.884
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=839
PRIMER_PAIR_20_PENALTY=2.686766
PRIMER_LEFT_20_PENALTY=1.802595
PRIMER_RIGHT_20_PENALTY=0.884171
PRIMER_LEFT_20_SEQUENCE=ACGAAGAAGTTGGTCTGATGG
PRIMER_RIGHT_20_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_20=194,21
PRIMER_RIGHT_20=1153,20
PRIMER_LEFT_20_TM=59.197
PRIMER_RIGHT_20_TM=60.884
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=960
PRIMER_PAIR_21_PENALTY=2.736805
PRIMER_LEFT_21_PENALTY=1.852633
PRIMER_RIGHT_21_PENALTY=0.884171
PRIMER_LEFT_21_SEQUENCE=CGTGTTTTTATCGGTCCTTG
PRIMER_RIGHT_21_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_21=328,20
PRIMER_RIGHT_21=1153,20
PRIMER_LEFT_21_TM=58.147
PRIMER_RIGHT_21_TM=60.884
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=826
PRIMER_PAIR_22_PENALTY=2.803389
PRIMER_LEFT_22_PENALTY=1.919218
PRIMER_RIGHT_22_PENALTY=0.884171
PRIMER_LEFT_22_SEQUENCE=GCTTTGTTGAGCGTGTTTTT
PRIMER_RIGHT_22_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_22=317,20
PRIMER_RIGHT_22=1153,20
PRIMER_LEFT_22_TM=58.081
PRIMER_RIGHT_22_TM=60.884
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=837
PRIMER_PAIR_23_PENALTY=2.875752
PRIMER_LEFT_23_PENALTY=1.991580
PRIMER_RIGHT_23_PENALTY=0.884171
PRIMER_LEFT_23_SEQUENCE=CGTTGTCAAGCAAAACTCCT
PRIMER_RIGHT_23_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_23=466,20
PRIMER_RIGHT_23=1153,20
PRIMER_LEFT_23_TM=58.008
PRIMER_RIGHT_23_TM=60.884
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=688
PRIMER_PAIR_24_PENALTY=2.915501
PRIMER_LEFT_24_PENALTY=2.031330
PRIMER_RIGHT_24_PENALTY=0.884171
PRIMER_LEFT_24_SEQUENCE=TGTTGAGCGTGTTTTTATCGG
PRIMER_RIGHT_24_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_24=321,21
PRIMER_RIGHT_24=1153,20
PRIMER_LEFT_24_TM=61.031
PRIMER_RIGHT_24_TM=60.884
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.8000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=833
PRIMER_PAIR_25_PENALTY=2.925270
PRIMER_LEFT_25_PENALTY=2.041099
PRIMER_RIGHT_25_PENALTY=0.884171
PRIMER_LEFT_25_SEQUENCE=TTTTTATCGGTCCTTGGGTTG
PRIMER_RIGHT_25_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_25=332,21
PRIMER_RIGHT_25=1153,20
PRIMER_LEFT_25_TM=61.041
PRIMER_RIGHT_25_TM=60.884
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=822
PRIMER_PAIR_26_PENALTY=2.931568
PRIMER_LEFT_26_PENALTY=2.047396
PRIMER_RIGHT_26_PENALTY=0.884171
PRIMER_LEFT_26_SEQUENCE=TTTTGGGATGGAAATCTCGG
PRIMER_RIGHT_26_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_26=542,20
PRIMER_RIGHT_26=1153,20
PRIMER_LEFT_26_TM=62.047
PRIMER_RIGHT_26_TM=60.884
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=612
PRIMER_PAIR_27_PENALTY=2.975037
PRIMER_LEFT_27_PENALTY=2.090865
PRIMER_RIGHT_27_PENALTY=0.884171
PRIMER_LEFT_27_SEQUENCE=AACCCTTGGAAGTGAGCTTT
PRIMER_RIGHT_27_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_27=440,20
PRIMER_RIGHT_27=1153,20
PRIMER_LEFT_27_TM=57.909
PRIMER_RIGHT_27_TM=60.884
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=714
PRIMER_PAIR_28_PENALTY=2.981395
PRIMER_LEFT_28_PENALTY=2.097224
PRIMER_RIGHT_28_PENALTY=0.884171
PRIMER_LEFT_28_SEQUENCE=GTTTTTATCGGTCCTTGGGT
PRIMER_RIGHT_28_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_28=331,20
PRIMER_RIGHT_28=1153,20
PRIMER_LEFT_28_TM=57.903
PRIMER_RIGHT_28_TM=60.884
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=823
PRIMER_PAIR_29_PENALTY=3.029690
PRIMER_LEFT_29_PENALTY=2.145518
PRIMER_RIGHT_29_PENALTY=0.884171
PRIMER_LEFT_29_SEQUENCE=ACCGTTGTCAAGCAAAACTC
PRIMER_RIGHT_29_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_29=464,20
PRIMER_RIGHT_29=1153,20
PRIMER_LEFT_29_TM=57.854
PRIMER_RIGHT_29_TM=60.884
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=690
PRIMER_PAIR_30_PENALTY=3.114420
PRIMER_LEFT_30_PENALTY=2.230249
PRIMER_RIGHT_30_PENALTY=0.884171
PRIMER_LEFT_30_SEQUENCE=TTCAAACCCTTGGAAGTGAG
PRIMER_RIGHT_30_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_30=436,20
PRIMER_RIGHT_30=1153,20
PRIMER_LEFT_30_TM=57.770
PRIMER_RIGHT_30_TM=60.884
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=718
PRIMER_PAIR_31_PENALTY=3.202550
PRIMER_LEFT_31_PENALTY=2.318379
PRIMER_RIGHT_31_PENALTY=0.884171
PRIMER_LEFT_31_SEQUENCE=GTTCAAACCCTTGGAAGTGAG
PRIMER_RIGHT_31_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_31=435,21
PRIMER_RIGHT_31=1153,20
PRIMER_LEFT_31_TM=58.682
PRIMER_RIGHT_31_TM=60.884
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=719
PRIMER_PAIR_32_PENALTY=3.313537
PRIMER_LEFT_32_PENALTY=2.429366
PRIMER_RIGHT_32_PENALTY=0.884171
PRIMER_LEFT_32_SEQUENCE=ATGGAAATCTCGGCGTCTTG
PRIMER_RIGHT_32_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_32=549,20
PRIMER_RIGHT_32=1153,20
PRIMER_LEFT_32_TM=62.429
PRIMER_RIGHT_32_TM=60.884
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=605
PRIMER_PAIR_33_PENALTY=3.331178
PRIMER_LEFT_33_PENALTY=2.447006
PRIMER_RIGHT_33_PENALTY=0.884171
PRIMER_LEFT_33_SEQUENCE=ACCGTTGTCAAGCAAAACTCC
PRIMER_RIGHT_33_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_33=464,21
PRIMER_RIGHT_33=1153,20
PRIMER_LEFT_33_TM=61.447
PRIMER_RIGHT_33_TM=60.884
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.6000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=690
PRIMER_PAIR_34_PENALTY=3.411957
PRIMER_LEFT_34_PENALTY=2.527786
PRIMER_RIGHT_34_PENALTY=0.884171
PRIMER_LEFT_34_SEQUENCE=GGCTTTGTTGAGCGTGTTTTT
PRIMER_RIGHT_34_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_34=316,21
PRIMER_RIGHT_34=1153,20
PRIMER_LEFT_34_TM=61.528
PRIMER_RIGHT_34_TM=60.884
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.6000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=838
PRIMER_PAIR_35_PENALTY=3.448454
PRIMER_LEFT_35_PENALTY=2.564282
PRIMER_RIGHT_35_PENALTY=0.884171
PRIMER_LEFT_35_SEQUENCE=CCGTTGTCAAGCAAAACTCCT
PRIMER_RIGHT_35_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_35=465,21
PRIMER_RIGHT_35=1153,20
PRIMER_LEFT_35_TM=61.564
PRIMER_RIGHT_35_TM=60.884
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=689
PRIMER_PAIR_36_PENALTY=3.494723
PRIMER_LEFT_36_PENALTY=2.610551
PRIMER_RIGHT_36_PENALTY=0.884171
PRIMER_LEFT_36_SEQUENCE=AACCGTTGTCAAGCAAAACT
PRIMER_RIGHT_36_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_36=463,20
PRIMER_RIGHT_36=1153,20
PRIMER_LEFT_36_TM=57.389
PRIMER_RIGHT_36_TM=60.884
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=691
PRIMER_PAIR_37_PENALTY=3.610605
PRIMER_LEFT_37_PENALTY=2.726433
PRIMER_RIGHT_37_PENALTY=0.884171
PRIMER_LEFT_37_SEQUENCE=GCGTGTTTTTATCGGTCCTTG
PRIMER_RIGHT_37_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_37=327,21
PRIMER_RIGHT_37=1153,20
PRIMER_LEFT_37_TM=61.726
PRIMER_RIGHT_37_TM=60.884
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=827
PRIMER_PAIR_38_PENALTY=3.752994
PRIMER_LEFT_38_PENALTY=2.868823
PRIMER_RIGHT_38_PENALTY=0.884171
PRIMER_LEFT_38_SEQUENCE=GAAGAAGTTGGTCTGATGGG
PRIMER_RIGHT_38_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_38=196,20
PRIMER_RIGHT_38=1153,20
PRIMER_LEFT_38_TM=57.131
PRIMER_RIGHT_38_TM=60.884
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=958
PRIMER_PAIR_39_PENALTY=3.770808
PRIMER_LEFT_39_PENALTY=2.886637
PRIMER_RIGHT_39_PENALTY=0.884171
PRIMER_LEFT_39_SEQUENCE=GTTGAGCGTGTTTTTATCGGTC
PRIMER_RIGHT_39_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_39=322,22
PRIMER_RIGHT_39=1153,20
PRIMER_LEFT_39_TM=60.887
PRIMER_RIGHT_39_TM=60.884
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=832
PRIMER_PAIR_40_PENALTY=3.826761
PRIMER_LEFT_40_PENALTY=2.942589
PRIMER_RIGHT_40_PENALTY=0.884171
PRIMER_LEFT_40_SEQUENCE=CGAAGAAGTTGGTCTGATGGG
PRIMER_RIGHT_40_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_40=195,21
PRIMER_RIGHT_40=1153,20
PRIMER_LEFT_40_TM=61.943
PRIMER_RIGHT_40_TM=60.884
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=959
PRIMER_PAIR_41_PENALTY=3.906054
PRIMER_LEFT_41_PENALTY=3.021883
PRIMER_RIGHT_41_PENALTY=0.884171
PRIMER_LEFT_41_SEQUENCE=TGGAAATCTCGGCGTCTTGT
PRIMER_RIGHT_41_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_41=550,20
PRIMER_RIGHT_41=1153,20
PRIMER_LEFT_41_TM=63.022
PRIMER_RIGHT_41_TM=60.884
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=604
PRIMER_PAIR_42_PENALTY=3.997270
PRIMER_LEFT_42_PENALTY=3.113098
PRIMER_RIGHT_42_PENALTY=0.884171
PRIMER_LEFT_42_SEQUENCE=TTGTTCACGAAGAAGTTGGTCT
PRIMER_RIGHT_42_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_42=188,22
PRIMER_RIGHT_42=1153,20
PRIMER_LEFT_42_TM=58.887
PRIMER_RIGHT_42_TM=60.884
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=966
PRIMER_PAIR_43_PENALTY=4.190650
PRIMER_LEFT_43_PENALTY=3.306479
PRIMER_RIGHT_43_PENALTY=0.884171
PRIMER_LEFT_43_SEQUENCE=GTGTTTTTATCGGTCCTTGGGT
PRIMER_RIGHT_43_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_43=329,22
PRIMER_RIGHT_43=1153,20
PRIMER_LEFT_43_TM=61.306
PRIMER_RIGHT_43_TM=60.884
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=825
PRIMER_PAIR_44_PENALTY=4.392063
PRIMER_LEFT_44_PENALTY=3.507892
PRIMER_RIGHT_44_PENALTY=0.884171
PRIMER_LEFT_44_SEQUENCE=GTGGCTTTGTTGAGCGTGTTT
PRIMER_RIGHT_44_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_44=314,21
PRIMER_RIGHT_44=1153,20
PRIMER_LEFT_44_TM=62.508
PRIMER_RIGHT_44_TM=60.884
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=840
PRIMER_PAIR_45_PENALTY=4.450541
PRIMER_LEFT_45_PENALTY=3.566369
PRIMER_RIGHT_45_PENALTY=0.884171
PRIMER_LEFT_45_SEQUENCE=TGTTCACGAAGAAGTTGGTCT
PRIMER_RIGHT_45_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_45=189,21
PRIMER_RIGHT_45=1153,20
PRIMER_LEFT_45_TM=57.434
PRIMER_RIGHT_45_TM=60.884
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=965
PRIMER_PAIR_46_PENALTY=4.552392
PRIMER_LEFT_46_PENALTY=3.668220
PRIMER_RIGHT_46_PENALTY=0.884171
PRIMER_LEFT_46_SEQUENCE=GAGCGTGTTTTTATCGGTCCTT
PRIMER_RIGHT_46_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_46=325,22
PRIMER_RIGHT_46=1153,20
PRIMER_LEFT_46_TM=61.668
PRIMER_RIGHT_46_TM=60.884
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=829
PRIMER_PAIR_47_PENALTY=4.567674
PRIMER_LEFT_47_PENALTY=3.683502
PRIMER_RIGHT_47_PENALTY=0.884171
PRIMER_LEFT_47_SEQUENCE=GGATGGAAATCTCGGCGTCT
PRIMER_RIGHT_47_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_47=547,20
PRIMER_RIGHT_47=1153,20
PRIMER_LEFT_47_TM=63.684
PRIMER_RIGHT_47_TM=60.884
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=607
PRIMER_PAIR_48_PENALTY=4.626986
PRIMER_LEFT_48_PENALTY=3.742815
PRIMER_RIGHT_48_PENALTY=0.884171
PRIMER_LEFT_48_SEQUENCE=GTTTTTATCGGTCCTTGGGTTG
PRIMER_RIGHT_48_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_48=331,22
PRIMER_RIGHT_48=1153,20
PRIMER_LEFT_48_TM=61.743
PRIMER_RIGHT_48_TM=60.884
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=823
PRIMER_PAIR_49_PENALTY=4.626986
PRIMER_LEFT_49_PENALTY=3.742815
PRIMER_RIGHT_49_PENALTY=0.884171
PRIMER_LEFT_49_SEQUENCE=TGTTTTTATCGGTCCTTGGGTT
PRIMER_RIGHT_49_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_49=330,22
PRIMER_RIGHT_49=1153,20
PRIMER_LEFT_49_TM=61.743
PRIMER_RIGHT_49_TM=60.884
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=824
=
SEQUENCE_ID=MAGIC.105_4_7353295_7353435_zu-0_hi-0_59760_57100_TRUE
SEQUENCE_TARGET=950,240
SEQUENCE_TEMPLATE=GAAACTTTTTCTACTCTAAAGACTTCAAAAATCATTCATTCATGTGNTAAAAAGGGATAAAACTTTCAACAAATCTAGAACAAAAACTCCTTTTCACAATGTCTATGACANCTAAAAGAATCGAAATTTTCTAGAAATTAAACTTCGAAGTATGCCCTGAAGCAGAAAAAAAAGATTGAAACTTTAGATGAATCTGAAATTTGAGACCACCAACAAACAAAGATAGAAACTTTATCTTNACCATTAATAGTAACCTAAAAGTTAGATCTTTAGCTCACTGAAAACAACAGTAAAATCTCTAGAAATTAAGGGTTTTACAAAATGTGGANAATCTATANGAAANACCCAAACTTTGATTCAANATCTNAAANCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATNTAAAGATCTNNNAGAAGAAGAAGAAGAAGAAACCTGTTGAGGNACACCAAGNGGAGAAGCAGTAATGTAAGAGACTTCGAAGTTATAGAAAGAATATGGATCTGCAGCGAAGCAGAAACTTATGTTCACGAAGAACACAAGAAGAAGGATCTTGAACAAATCCATTTTCNTTTTTTTCTCTCGCAAAATCTTTTGCTTTGNTNTTTNGTTGAGAAGCAATGTTGGGTTTTGATTGGTTGTTTTTAAACAGATTTGTTGTGAATTTACATTATCAAGATTCGGGAAAAAGGAAGTGAGAAGTGGGAGAAACTAGANGCTATAGAAGAAAGAGAAGAGAGCCAGAGAGTGATATAGACATAAGAGAACGGCTACAAAATTCTTAGGTTGCTCCTTAATTATNTANTATTATTANNTTTTGACTATTATTTTACCAGAAATAAAGCCGCACAATACGCCTATAAATTTGCCTAAATATGAGAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATAAATAAATTTNAGGGTATANTTACATATAANNTTATTANNTTTNAAATTGATGTTGCNATNAGTNTGNNCTAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNTTTTNTTTTNNTTNNNNNNNNNNNNNNNNNNNNNNACANTAGTTNATNAATANTNAATAAAAANNAATNNGGTTNNNNNNNNNTTTTGTNNGNTTNGACNNNTATANNNNNNNNNNNNNNNNNNNNNNNNNTNAAATCACTAAGATTATGATNAATGTNAATGTAGTCTCACATTCTTCTACTTNAANCTATTTACTTAATTTNNAAAAAGTAGTTAGTTAATTAATCAAAAGAAATAGTAAAGNGNANATAANCTAAATAGTATGATTNAANTTTGCTAAGACNATCATTTATNACTTTAGTATCATATATTCGAATAACATTCTTCATACTTTTCNTTTTAATATATTACNNNNTAAACCACTGNATTTATAGATACCTTATCCTTCATTAGAGAGTTTTAATCTTCTTTTNATCGTCGGTGANCATTANTTTATTATATTTAAGCAAAATNAATANTAAGTTGACACTTGAAAANNAAANNAAAAANAAAGCCAACGGATATAATGATAATAGTACTGCATGACAGCNAGAAAAGAATGGCTCCCATATGGTTGACAATTTTTTATATACTTTTTATTTTCCACCGANATNTTTTCTCTTATATGCTTTATTTATCAAAATGAGCTGTAAAATTTCNTTTCATAAACCNNATAATTTTTCTTATAAAATATTCTAACGCGTATTTTCTGATTGATGATCNAACATTTACGTTGATTGTGGTGNAATTATTGTTATGAAATAATGATGCATTAAAAAAGAANAAGATTTTGATTTAGTAATTTGTCGGTTAGGGAAGTGCATTATGTACGCAATAATCACATGCTGCTCGCGTGCTAGCTTAGGTTTAAATAAGTGAATTAAGTACTAGTAGTAACTTNATAGTTACATTATTGTGTTAATATTTGATTCTTACNACAAGTTTTAAATTAAGATTTGATATCTGACCNTTTGACCAAAGGCATCTGATAAAGAGATAAGATTTATTGCGCAACGCAACGGTGGGCTACTCNNNAAATGCCACGTGGTTTATGCCAGCCAAAACGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.695059
PRIMER_LEFT_0_PENALTY=0.215902
PRIMER_RIGHT_0_PENALTY=2.479156
PRIMER_LEFT_0_SEQUENCE=TCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_0_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_0=687,20
PRIMER_RIGHT_0=1645,21
PRIMER_LEFT_0_TM=59.784
PRIMER_RIGHT_0_TM=58.521
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=959
PRIMER_PAIR_1_PENALTY=2.752666
PRIMER_LEFT_1_PENALTY=0.215902
PRIMER_RIGHT_1_PENALTY=2.536763
PRIMER_LEFT_1_SEQUENCE=TCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_1_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_1=687,20
PRIMER_RIGHT_1=1645,22
PRIMER_LEFT_1_TM=59.784
PRIMER_RIGHT_1_TM=59.463
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=959
PRIMER_PAIR_2_PENALTY=3.195055
PRIMER_LEFT_2_PENALTY=0.715898
PRIMER_RIGHT_2_PENALTY=2.479156
PRIMER_LEFT_2_SEQUENCE=CGGGAAAAAGGAAGTGAGAA
PRIMER_RIGHT_2_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_2=688,20
PRIMER_RIGHT_2=1645,21
PRIMER_LEFT_2_TM=59.284
PRIMER_RIGHT_2_TM=58.521
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=958
PRIMER_PAIR_3_PENALTY=3.195055
PRIMER_LEFT_3_PENALTY=0.715898
PRIMER_RIGHT_3_PENALTY=2.479156
PRIMER_LEFT_3_SEQUENCE=TTCGGGAAAAAGGAAGTGAG
PRIMER_RIGHT_3_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_3=686,20
PRIMER_RIGHT_3=1645,21
PRIMER_LEFT_3_TM=59.284
PRIMER_RIGHT_3_TM=58.521
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=960
PRIMER_PAIR_4_PENALTY=3.560708
PRIMER_LEFT_4_PENALTY=0.215902
PRIMER_RIGHT_4_PENALTY=3.344806
PRIMER_LEFT_4_SEQUENCE=TCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_4_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_4=687,20
PRIMER_RIGHT_4=1645,20
PRIMER_LEFT_4_TM=59.784
PRIMER_RIGHT_4_TM=56.655
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=959
PRIMER_PAIR_5_PENALTY=3.599621
PRIMER_LEFT_5_PENALTY=1.120465
PRIMER_RIGHT_5_PENALTY=2.479156
PRIMER_LEFT_5_SEQUENCE=CCAGAAATAAAGCCGCACAA
PRIMER_RIGHT_5_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_5=839,20
PRIMER_RIGHT_5=1645,21
PRIMER_LEFT_5_TM=61.120
PRIMER_RIGHT_5_TM=58.521
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=807
PRIMER_PAIR_6_PENALTY=3.756895
PRIMER_LEFT_6_PENALTY=1.220131
PRIMER_RIGHT_6_PENALTY=2.536763
PRIMER_LEFT_6_SEQUENCE=CGGGAAAAAGGAAGTGAGAAG
PRIMER_RIGHT_6_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_6=688,21
PRIMER_RIGHT_6=1645,22
PRIMER_LEFT_6_TM=60.220
PRIMER_RIGHT_6_TM=59.463
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=958
PRIMER_PAIR_7_PENALTY=3.835049
PRIMER_LEFT_7_PENALTY=0.215902
PRIMER_RIGHT_7_PENALTY=3.619147
PRIMER_LEFT_7_SEQUENCE=TCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_7_SEQUENCE=TATGGGAGCCATTCTTTTCT
PRIMER_LEFT_7=687,20
PRIMER_RIGHT_7=1643,20
PRIMER_LEFT_7_TM=59.784
PRIMER_RIGHT_7_TM=56.381
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=957
PRIMER_PAIR_8_PENALTY=3.858727
PRIMER_LEFT_8_PENALTY=1.379571
PRIMER_RIGHT_8_PENALTY=2.479156
PRIMER_LEFT_8_SEQUENCE=ATTCGGGAAAAAGGAAGTGA
PRIMER_RIGHT_8_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_8=685,20
PRIMER_RIGHT_8=1645,21
PRIMER_LEFT_8_TM=58.620
PRIMER_RIGHT_8_TM=58.521
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=961
PRIMER_PAIR_9_PENALTY=3.896062
PRIMER_LEFT_9_PENALTY=1.416906
PRIMER_RIGHT_9_PENALTY=2.479156
PRIMER_LEFT_9_SEQUENCE=GATTCGGGAAAAAGGAAGTGA
PRIMER_RIGHT_9_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_9=684,21
PRIMER_RIGHT_9=1645,21
PRIMER_LEFT_9_TM=60.417
PRIMER_RIGHT_9_TM=58.521
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=962
PRIMER_PAIR_10_PENALTY=3.899453
PRIMER_LEFT_10_PENALTY=1.420297
PRIMER_RIGHT_10_PENALTY=2.479156
PRIMER_LEFT_10_SEQUENCE=ATTCGGGAAAAAGGAAGTGAG
PRIMER_RIGHT_10_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_10=685,21
PRIMER_RIGHT_10=1645,21
PRIMER_LEFT_10_TM=59.580
PRIMER_RIGHT_10_TM=58.521
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=961
PRIMER_PAIR_11_PENALTY=4.034356
PRIMER_LEFT_11_PENALTY=1.555199
PRIMER_RIGHT_11_PENALTY=2.479156
PRIMER_LEFT_11_SEQUENCE=TTACCAGAAATAAAGCCGCA
PRIMER_RIGHT_11_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_11=836,20
PRIMER_RIGHT_11=1645,21
PRIMER_LEFT_11_TM=58.445
PRIMER_RIGHT_11_TM=58.521
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=11.7000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=810
PRIMER_PAIR_12_PENALTY=4.060704
PRIMER_LEFT_12_PENALTY=0.715898
PRIMER_RIGHT_12_PENALTY=3.344806
PRIMER_LEFT_12_SEQUENCE=CGGGAAAAAGGAAGTGAGAA
PRIMER_RIGHT_12_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_12=688,20
PRIMER_RIGHT_12=1645,20
PRIMER_LEFT_12_TM=59.284
PRIMER_RIGHT_12_TM=56.655
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=958
PRIMER_PAIR_13_PENALTY=4.091963
PRIMER_LEFT_13_PENALTY=1.555199
PRIMER_RIGHT_13_PENALTY=2.536763
PRIMER_LEFT_13_SEQUENCE=TTACCAGAAATAAAGCCGCA
PRIMER_RIGHT_13_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_13=836,20
PRIMER_RIGHT_13=1645,22
PRIMER_LEFT_13_TM=58.445
PRIMER_RIGHT_13_TM=59.463
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.7000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=810
PRIMER_PAIR_14_PENALTY=4.213003
PRIMER_LEFT_14_PENALTY=1.733846
PRIMER_RIGHT_14_PENALTY=2.479156
PRIMER_LEFT_14_SEQUENCE=TTACCAGAAATAAAGCCGCAC
PRIMER_RIGHT_14_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_14=836,21
PRIMER_RIGHT_14=1645,21
PRIMER_LEFT_14_TM=59.266
PRIMER_RIGHT_14_TM=58.521
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=810
PRIMER_PAIR_15_PENALTY=4.369272
PRIMER_LEFT_15_PENALTY=1.890116
PRIMER_RIGHT_15_PENALTY=2.479156
PRIMER_LEFT_15_SEQUENCE=ATCAAGATTCGGGAAAAAGG
PRIMER_RIGHT_15_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_15=679,20
PRIMER_RIGHT_15=1645,21
PRIMER_LEFT_15_TM=58.110
PRIMER_RIGHT_15_TM=58.521
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=7.3000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=967
PRIMER_PAIR_16_PENALTY=4.426879
PRIMER_LEFT_16_PENALTY=1.890116
PRIMER_RIGHT_16_PENALTY=2.536763
PRIMER_LEFT_16_SEQUENCE=ATCAAGATTCGGGAAAAAGG
PRIMER_RIGHT_16_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_16=679,20
PRIMER_RIGHT_16=1645,22
PRIMER_LEFT_16_TM=58.110
PRIMER_RIGHT_16_TM=59.463
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=967
PRIMER_PAIR_17_PENALTY=4.465271
PRIMER_LEFT_17_PENALTY=1.120465
PRIMER_RIGHT_17_PENALTY=3.344806
PRIMER_LEFT_17_SEQUENCE=CCAGAAATAAAGCCGCACAA
PRIMER_RIGHT_17_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_17=839,20
PRIMER_RIGHT_17=1645,20
PRIMER_LEFT_17_TM=61.120
PRIMER_RIGHT_17_TM=56.655
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=807
PRIMER_PAIR_18_PENALTY=4.481963
PRIMER_LEFT_18_PENALTY=0.215902
PRIMER_RIGHT_18_PENALTY=4.266060
PRIMER_LEFT_18_SEQUENCE=TCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_18_SEQUENCE=ATATGGGAGCCATTCTTTTC
PRIMER_LEFT_18=687,20
PRIMER_RIGHT_18=1644,20
PRIMER_LEFT_18_TM=59.784
PRIMER_RIGHT_18_TM=55.734
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=958
PRIMER_PAIR_19_PENALTY=4.552342
PRIMER_LEFT_19_PENALTY=2.073186
PRIMER_RIGHT_19_PENALTY=2.479156
PRIMER_LEFT_19_SEQUENCE=TCGGGAAAAAGGAAGTGAGAA
PRIMER_RIGHT_19_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_19=687,21
PRIMER_RIGHT_19=1645,21
PRIMER_LEFT_19_TM=61.073
PRIMER_RIGHT_19_TM=58.521
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=959
PRIMER_PAIR_20_PENALTY=4.552342
PRIMER_LEFT_20_PENALTY=2.073186
PRIMER_RIGHT_20_PENALTY=2.479156
PRIMER_LEFT_20_SEQUENCE=TTCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_20_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_20=686,21
PRIMER_RIGHT_20=1645,21
PRIMER_LEFT_20_TM=61.073
PRIMER_RIGHT_20_TM=58.521
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=960
PRIMER_PAIR_21_PENALTY=4.552855
PRIMER_LEFT_21_PENALTY=2.073699
PRIMER_RIGHT_21_PENALTY=2.479156
PRIMER_LEFT_21_SEQUENCE=TACCAGAAATAAAGCCGCAC
PRIMER_RIGHT_21_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_21=837,20
PRIMER_RIGHT_21=1645,21
PRIMER_LEFT_21_TM=57.926
PRIMER_RIGHT_21_TM=58.521
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=809
PRIMER_PAIR_22_PENALTY=4.564937
PRIMER_LEFT_22_PENALTY=1.220131
PRIMER_RIGHT_22_PENALTY=3.344806
PRIMER_LEFT_22_SEQUENCE=CGGGAAAAAGGAAGTGAGAAG
PRIMER_RIGHT_22_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_22=688,21
PRIMER_RIGHT_22=1645,20
PRIMER_LEFT_22_TM=60.220
PRIMER_RIGHT_22_TM=56.655
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=958
PRIMER_PAIR_23_PENALTY=4.609949
PRIMER_LEFT_23_PENALTY=2.073186
PRIMER_RIGHT_23_PENALTY=2.536763
PRIMER_LEFT_23_SEQUENCE=TTCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_23_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_23=686,21
PRIMER_RIGHT_23=1645,22
PRIMER_LEFT_23_TM=61.073
PRIMER_RIGHT_23_TM=59.463
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=960
PRIMER_PAIR_24_PENALTY=4.678757
PRIMER_LEFT_24_PENALTY=2.199601
PRIMER_RIGHT_24_PENALTY=2.479156
PRIMER_LEFT_24_SEQUENCE=ATAAAGCCGCACAATACGCC
PRIMER_RIGHT_24_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_24=845,20
PRIMER_RIGHT_24=1645,21
PRIMER_LEFT_24_TM=62.200
PRIMER_RIGHT_24_TM=58.521
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=11.1000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=801
PRIMER_PAIR_25_PENALTY=4.736364
PRIMER_LEFT_25_PENALTY=2.199601
PRIMER_RIGHT_25_PENALTY=2.536763
PRIMER_LEFT_25_SEQUENCE=ATAAAGCCGCACAATACGCC
PRIMER_RIGHT_25_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_25=845,20
PRIMER_RIGHT_25=1645,22
PRIMER_LEFT_25_TM=62.200
PRIMER_RIGHT_25_TM=59.463
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=11.1000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=801
PRIMER_PAIR_26_PENALTY=4.839278
PRIMER_LEFT_26_PENALTY=1.220131
PRIMER_RIGHT_26_PENALTY=3.619147
PRIMER_LEFT_26_SEQUENCE=CGGGAAAAAGGAAGTGAGAAG
PRIMER_RIGHT_26_SEQUENCE=TATGGGAGCCATTCTTTTCT
PRIMER_LEFT_26=688,21
PRIMER_RIGHT_26=1643,20
PRIMER_LEFT_26_TM=60.220
PRIMER_RIGHT_26_TM=56.381
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=956
PRIMER_PAIR_27_PENALTY=4.900005
PRIMER_LEFT_27_PENALTY=1.555199
PRIMER_RIGHT_27_PENALTY=3.344806
PRIMER_LEFT_27_SEQUENCE=TTACCAGAAATAAAGCCGCA
PRIMER_RIGHT_27_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_27=836,20
PRIMER_RIGHT_27=1645,20
PRIMER_LEFT_27_TM=58.445
PRIMER_RIGHT_27_TM=56.655
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=11.7000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=810
PRIMER_PAIR_28_PENALTY=4.956165
PRIMER_LEFT_28_PENALTY=2.477009
PRIMER_RIGHT_28_PENALTY=2.479156
PRIMER_LEFT_28_SEQUENCE=TTTACCAGAAATAAAGCCGCAC
PRIMER_RIGHT_28_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_28=835,22
PRIMER_RIGHT_28=1645,21
PRIMER_LEFT_28_TM=60.477
PRIMER_RIGHT_28_TM=58.521
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.9000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=811
PRIMER_PAIR_29_PENALTY=4.981959
PRIMER_LEFT_29_PENALTY=0.715898
PRIMER_RIGHT_29_PENALTY=4.266060
PRIMER_LEFT_29_SEQUENCE=CGGGAAAAAGGAAGTGAGAA
PRIMER_RIGHT_29_SEQUENCE=ATATGGGAGCCATTCTTTTC
PRIMER_LEFT_29=688,20
PRIMER_RIGHT_29=1644,20
PRIMER_LEFT_29_TM=59.284
PRIMER_RIGHT_29_TM=55.734
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=957
PRIMER_PAIR_30_PENALTY=4.981959
PRIMER_LEFT_30_PENALTY=0.715898
PRIMER_RIGHT_30_PENALTY=4.266060
PRIMER_LEFT_30_SEQUENCE=TTCGGGAAAAAGGAAGTGAG
PRIMER_RIGHT_30_SEQUENCE=ATATGGGAGCCATTCTTTTC
PRIMER_LEFT_30=686,20
PRIMER_RIGHT_30=1644,20
PRIMER_LEFT_30_TM=59.284
PRIMER_RIGHT_30_TM=55.734
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.3000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=959
PRIMER_PAIR_31_PENALTY=5.078652
PRIMER_LEFT_31_PENALTY=1.733846
PRIMER_RIGHT_31_PENALTY=3.344806
PRIMER_LEFT_31_SEQUENCE=TTACCAGAAATAAAGCCGCAC
PRIMER_RIGHT_31_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_31=836,21
PRIMER_RIGHT_31=1645,20
PRIMER_LEFT_31_TM=59.266
PRIMER_RIGHT_31_TM=56.655
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.9000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=810
PRIMER_PAIR_32_PENALTY=5.114327
PRIMER_LEFT_32_PENALTY=2.635171
PRIMER_RIGHT_32_PENALTY=2.479156
PRIMER_LEFT_32_SEQUENCE=AAGGAAGTGAGAAGTGGGAGAA
PRIMER_RIGHT_32_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_32=695,22
PRIMER_RIGHT_32=1645,21
PRIMER_LEFT_32_TM=59.365
PRIMER_RIGHT_32_TM=58.521
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=1.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=951
PRIMER_PAIR_33_PENALTY=5.114327
PRIMER_LEFT_33_PENALTY=2.635171
PRIMER_RIGHT_33_PENALTY=2.479156
PRIMER_LEFT_33_SEQUENCE=AAAGGAAGTGAGAAGTGGGAGA
PRIMER_RIGHT_33_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_33=694,22
PRIMER_RIGHT_33=1645,21
PRIMER_LEFT_33_TM=59.365
PRIMER_RIGHT_33_TM=58.521
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=1.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=952
PRIMER_PAIR_34_PENALTY=5.171934
PRIMER_LEFT_34_PENALTY=2.635171
PRIMER_RIGHT_34_PENALTY=2.536763
PRIMER_LEFT_34_SEQUENCE=AAAGGAAGTGAGAAGTGGGAGA
PRIMER_RIGHT_34_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_34=694,22
PRIMER_RIGHT_34=1645,22
PRIMER_LEFT_34_TM=59.365
PRIMER_RIGHT_34_TM=59.463
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=1.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=952
PRIMER_PAIR_35_PENALTY=5.174346
PRIMER_LEFT_35_PENALTY=1.555199
PRIMER_RIGHT_35_PENALTY=3.619147
PRIMER_LEFT_35_SEQUENCE=TTACCAGAAATAAAGCCGCA
PRIMER_RIGHT_35_SEQUENCE=TATGGGAGCCATTCTTTTCT
PRIMER_LEFT_35=836,20
PRIMER_RIGHT_35=1643,20
PRIMER_LEFT_35_TM=58.445
PRIMER_RIGHT_35_TM=56.381
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=11.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=808
PRIMER_PAIR_36_PENALTY=5.223930
PRIMER_LEFT_36_PENALTY=2.744773
PRIMER_RIGHT_36_PENALTY=2.479156
PRIMER_LEFT_36_SEQUENCE=AAAAAGGAAGTGAGAAGTGGGA
PRIMER_RIGHT_36_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_36=692,22
PRIMER_RIGHT_36=1645,21
PRIMER_LEFT_36_TM=59.255
PRIMER_RIGHT_36_TM=58.521
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=1.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=954
PRIMER_PAIR_37_PENALTY=5.223930
PRIMER_LEFT_37_PENALTY=2.744773
PRIMER_RIGHT_37_PENALTY=2.479156
PRIMER_LEFT_37_SEQUENCE=GAAAAAGGAAGTGAGAAGTGGG
PRIMER_RIGHT_37_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_37=691,22
PRIMER_RIGHT_37=1645,21
PRIMER_LEFT_37_TM=59.255
PRIMER_RIGHT_37_TM=58.521
PRIMER_LEFT_37_GC_PERCENT=45.455
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=1.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=955
PRIMER_PAIR_38_PENALTY=5.223930
PRIMER_LEFT_38_PENALTY=2.744773
PRIMER_RIGHT_38_PENALTY=2.479156
PRIMER_LEFT_38_SEQUENCE=GGAAAAAGGAAGTGAGAAGTGG
PRIMER_RIGHT_38_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_38=690,22
PRIMER_RIGHT_38=1645,21
PRIMER_LEFT_38_TM=59.255
PRIMER_RIGHT_38_TM=58.521
PRIMER_LEFT_38_GC_PERCENT=45.455
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=1.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=7.3000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=956
PRIMER_PAIR_39_PENALTY=5.223930
PRIMER_LEFT_39_PENALTY=2.744773
PRIMER_RIGHT_39_PENALTY=2.479156
PRIMER_LEFT_39_SEQUENCE=GGGAAAAAGGAAGTGAGAAGTG
PRIMER_RIGHT_39_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_39=689,22
PRIMER_RIGHT_39=1645,21
PRIMER_LEFT_39_TM=59.255
PRIMER_RIGHT_39_TM=58.521
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=1.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=957
PRIMER_PAIR_40_PENALTY=5.248666
PRIMER_LEFT_40_PENALTY=2.769510
PRIMER_RIGHT_40_PENALTY=2.479156
PRIMER_LEFT_40_SEQUENCE=GGAAGTGAGAAGTGGGAGAAAC
PRIMER_RIGHT_40_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_40=697,22
PRIMER_RIGHT_40=1645,21
PRIMER_LEFT_40_TM=59.230
PRIMER_RIGHT_40_TM=58.521
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.3000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=949
PRIMER_PAIR_41_PENALTY=5.267959
PRIMER_LEFT_41_PENALTY=2.788802
PRIMER_RIGHT_41_PENALTY=2.479156
PRIMER_LEFT_41_SEQUENCE=CAAGATTCGGGAAAAAGGAAGT
PRIMER_RIGHT_41_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_41=681,22
PRIMER_RIGHT_41=1645,21
PRIMER_LEFT_41_TM=60.789
PRIMER_RIGHT_41_TM=58.521
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.3000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=965
PRIMER_PAIR_42_PENALTY=5.281537
PRIMER_LEFT_42_PENALTY=2.744773
PRIMER_RIGHT_42_PENALTY=2.536763
PRIMER_LEFT_42_SEQUENCE=AAAAAGGAAGTGAGAAGTGGGA
PRIMER_RIGHT_42_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_42=692,22
PRIMER_RIGHT_42=1645,22
PRIMER_LEFT_42_TM=59.255
PRIMER_RIGHT_42_TM=59.463
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=1.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=954
PRIMER_PAIR_43_PENALTY=5.281537
PRIMER_LEFT_43_PENALTY=2.744773
PRIMER_RIGHT_43_PENALTY=2.536763
PRIMER_LEFT_43_SEQUENCE=GAAAAAGGAAGTGAGAAGTGGG
PRIMER_RIGHT_43_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_43=691,22
PRIMER_RIGHT_43=1645,22
PRIMER_LEFT_43_TM=59.255
PRIMER_RIGHT_43_TM=59.463
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=1.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=955
PRIMER_PAIR_44_PENALTY=5.281537
PRIMER_LEFT_44_PENALTY=2.744773
PRIMER_RIGHT_44_PENALTY=2.536763
PRIMER_LEFT_44_SEQUENCE=GGAAAAAGGAAGTGAGAAGTGG
PRIMER_RIGHT_44_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_44=690,22
PRIMER_RIGHT_44=1645,22
PRIMER_LEFT_44_TM=59.255
PRIMER_RIGHT_44_TM=59.463
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=1.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=956
PRIMER_PAIR_45_PENALTY=5.281537
PRIMER_LEFT_45_PENALTY=2.744773
PRIMER_RIGHT_45_PENALTY=2.536763
PRIMER_LEFT_45_SEQUENCE=GGGAAAAAGGAAGTGAGAAGTG
PRIMER_RIGHT_45_SEQUENCE=CATATGGGAGCCATTCTTTTCT
PRIMER_LEFT_45=689,22
PRIMER_RIGHT_45=1645,22
PRIMER_LEFT_45_TM=59.255
PRIMER_RIGHT_45_TM=59.463
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=1.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=957
PRIMER_PAIR_46_PENALTY=5.329586
PRIMER_LEFT_46_PENALTY=2.850430
PRIMER_RIGHT_46_PENALTY=2.479156
PRIMER_LEFT_46_SEQUENCE=ACCAGAAATAAAGCCGCACAA
PRIMER_RIGHT_46_SEQUENCE=CATATGGGAGCCATTCTTTTC
PRIMER_LEFT_46=838,21
PRIMER_RIGHT_46=1645,21
PRIMER_LEFT_46_TM=61.850
PRIMER_RIGHT_46_TM=58.521
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=808
PRIMER_PAIR_47_PENALTY=5.386525
PRIMER_LEFT_47_PENALTY=1.120465
PRIMER_RIGHT_47_PENALTY=4.266060
PRIMER_LEFT_47_SEQUENCE=CCAGAAATAAAGCCGCACAA
PRIMER_RIGHT_47_SEQUENCE=ATATGGGAGCCATTCTTTTC
PRIMER_LEFT_47=839,20
PRIMER_RIGHT_47=1644,20
PRIMER_LEFT_47_TM=61.120
PRIMER_RIGHT_47_TM=55.734
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=806
PRIMER_PAIR_48_PENALTY=5.417992
PRIMER_LEFT_48_PENALTY=2.073186
PRIMER_RIGHT_48_PENALTY=3.344806
PRIMER_LEFT_48_SEQUENCE=TCGGGAAAAAGGAAGTGAGAA
PRIMER_RIGHT_48_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_48=687,21
PRIMER_RIGHT_48=1645,20
PRIMER_LEFT_48_TM=61.073
PRIMER_RIGHT_48_TM=56.655
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=959
PRIMER_PAIR_49_PENALTY=5.417992
PRIMER_LEFT_49_PENALTY=2.073186
PRIMER_RIGHT_49_PENALTY=3.344806
PRIMER_LEFT_49_SEQUENCE=TTCGGGAAAAAGGAAGTGAGA
PRIMER_RIGHT_49_SEQUENCE=CATATGGGAGCCATTCTTTT
PRIMER_LEFT_49=686,21
PRIMER_RIGHT_49=1645,20
PRIMER_LEFT_49_TM=61.073
PRIMER_RIGHT_49_TM=56.655
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=960
=
SEQUENCE_ID=MAGIC.149_5_16560608_16561088_wil-2_wu-0_453425_721950_TRUE
SEQUENCE_TARGET=950,580
SEQUENCE_TEMPLATE=GTGTGNTAGGTGATTNGTAATACTATAATTTATTTGTATTATTGTTGTTGTTTTCTAATTTATTATCATGGTTTACTAATGTCNGTTGGATTTGACATCACCTTTCATATTACCAAAATTAGATTATTTATGCTCCTTGNAAGTGCTATTTTTCAGTCCTTTTGAATTTTTTGCTCCACAATGGAAAAGAAACAATTTTAAATACAAGAAAAACACATGTGAGCTTCNCCGTTTCTTCTTCTGTTTTATTTCTCTTTTCCGATTAGAGGGTTTTATTCAGTCAAATTNCCAATTATGTTAAAGGCGTATTTCCTGAAGTGTTACTTAATGATAAGAGTTGATTTACTAGAATAATATTATTTAAATTGGGGAAAACTGAATTGTGATTGGCGATAGGAGAAAGGGATATATTATGATATCATAGTTTAACATTTATATTAATTTTGTCAANAAAGTTTACTGGTCNTTTTCTCCAATTTTACAGAGATAAAAGTCGAATTCGAGCACATCAATTACCAACACGCGGGACTGATCAAATCACTCATGCGCACATCTTCCTCAGTATTGCAATAACATTTTNNNTNTTTTTNTTTTTTGGGNAAAAAGAAGTGAGCATTTTTTTCATTCCTTTGGTNTGGGATCANNNNNNNNNNNNNNNNTGTAGCCGCCANTTATCAATATATCGTTCATCANNATTATTCATGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4572486_4572626_sf-2_wu-0_20461_42036_TRUE
SEQUENCE_TARGET=950,240
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGNGAATGAAGAAACTTTGGTCCATGTTAGCTAGTTTAAAATAGTTTAAGACTAGACTAATCAACTTGTGTTATGTAATTCAGAATCAGACTTGTTGTCTNCTTGNNNNNNNNNNNNNNATTTAGACTTATGTTATGTTGAATGATTTGTATGGATTTTTCCTCTATGTTGGATTTGATTCAGACTTAAGATTAAANTTTTANTCANAGNNTTTNNATAGACCAGTCCTATTTTCTCACCAAAGNACATTGANTNTTNTTTCTGCTGNGANACACAAGTGTTTCAATTGTAGGTTTCATTACATGATCAATTCTACCTTCATNTACTTGTTCCACTTCAGTCCATCCNTNACTNCAATCCTTCTTNCTCATTTTATACCCTTTNATCACAAGATCAAAAACATGTAACAGATCCTTGNTNNNNNNNNNNNNNNNNNAAAACCAAGTGGTCATATTCATNAGAATTAACACCAACTNTTGGTTTCACTTCAACCTGCAAATATATCNCTCGCAAAATTGTGAGGTANTTGCNNACCGAAAACCCTAGATGTTGGTATAANAGAGAAGAGATTCGATAANANANAAAATCGAACAAACCCANNNATAAAACTTTAGTNTCATAAANTTNTAACCTTCTATTGATTCCTTTGCTGGAGAAACAAAATTTCTTCAANTCTAAGNAAGAAGACATAGTGAGAGAGACNACNGNTTTNNNNNACCAGTCCTATTTTNTNNAGNACATTGANNNTNTTTTTCTGCNATGATACACAAGTGTTTCAATTGNNNNNNNNNNNNCATGATCAATTCTACCTTCATGTANTTGTTCCACTTCAATCCATCCNTGACTCCAATCNTTCTTGCTCATTTTATACCCTTTGNTCACAAGATNNNAAANNTGTAACAGATNCTNGTNTTTAGGATNCANTTNTGAAACACANGGGTCATNTTNATGAGAATTAACANCAANTATTGGTTTCACNNNNNNNTGCAAATATANTCCTCGTNAAATTTCNAGGTACTTGCGNGNNNNNNNNNNNGAATTTTCTCNCAAATTCGTCGCAGAATTGNNGGGACTTTGCGAGCAGATAGGAGAACGTCNNNNNNNNNNNGCAAAATTTGCGANNAATATATCCCTCGCAAAATTTTCTTCTTCCTCTTNTTTTTCTTCTTCTCTAGTAGAACTGGCANNAACTCTTTCNATNCCTANNNAGTANTCCATCCTTCNAACTTTGTACCTAAGAACTTCACANTTTTCATAGACAAGTCCTATTTTCTCACCAAAGGACATTNATTTCCTTTTTCTGCNGCNATANACAAGTNNNNNNNNNNNNNGTTTCATTACATGATCAGTTCTACCTTCATGTGCTTGTTTCACTTCAATCCATCCCTGACTCCAATCCTTCTTGCTCATTTTATATCCTTTGTTCANAAGATAAAAAATATGTAACAGATCCTTGTCCTTAGGATTCTTTTTTGAAACCAAGTGGTCATATTCATNAGAATTAACACCAAGTATTGGTTTCACTTCAACCTNCAAATATATCNNTCGCAAAATTGTGAGGTANTTGCNAACCGAAAACCCTAAATATTGTTATAACAGAGAAGAGATTCGNTAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.064086
PRIMER_LEFT_0_PENALTY=1.041745
PRIMER_RIGHT_0_PENALTY=0.022341
PRIMER_LEFT_0_SEQUENCE=TTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_0_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_0=851,21
PRIMER_RIGHT_0=1612,20
PRIMER_LEFT_0_TM=59.958
PRIMER_RIGHT_0_TM=59.978
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=9.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=762
PRIMER_PAIR_1_PENALTY=1.064086
PRIMER_LEFT_1_PENALTY=1.041745
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=TTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_1_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_1=851,21
PRIMER_RIGHT_1=1613,20
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=763
PRIMER_PAIR_2_PENALTY=1.540035
PRIMER_LEFT_2_PENALTY=1.517694
PRIMER_RIGHT_2_PENALTY=0.022341
PRIMER_LEFT_2_SEQUENCE=TGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_2_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_2=852,20
PRIMER_RIGHT_2=1612,20
PRIMER_LEFT_2_TM=58.482
PRIMER_RIGHT_2_TM=59.978
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=761
PRIMER_PAIR_3_PENALTY=1.540035
PRIMER_LEFT_3_PENALTY=1.517694
PRIMER_RIGHT_3_PENALTY=0.022341
PRIMER_LEFT_3_SEQUENCE=TGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_3_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_3=852,20
PRIMER_RIGHT_3=1613,20
PRIMER_LEFT_3_TM=58.482
PRIMER_RIGHT_3_TM=59.978
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=762
PRIMER_PAIR_4_PENALTY=2.809333
PRIMER_LEFT_4_PENALTY=1.041745
PRIMER_RIGHT_4_PENALTY=1.767588
PRIMER_LEFT_4_SEQUENCE=TTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_4_SEQUENCE=CTCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_4=851,21
PRIMER_RIGHT_4=1615,21
PRIMER_LEFT_4_TM=59.958
PRIMER_RIGHT_4_TM=59.232
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=765
PRIMER_PAIR_5_PENALTY=2.840871
PRIMER_LEFT_5_PENALTY=1.041745
PRIMER_RIGHT_5_PENALTY=1.799127
PRIMER_LEFT_5_SEQUENCE=TTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_5_SEQUENCE=TCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_5=851,21
PRIMER_RIGHT_5=1614,20
PRIMER_LEFT_5_TM=59.958
PRIMER_RIGHT_5_TM=58.201
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=764
PRIMER_PAIR_6_PENALTY=2.934433
PRIMER_LEFT_6_PENALTY=2.912092
PRIMER_RIGHT_6_PENALTY=0.022341
PRIMER_LEFT_6_SEQUENCE=CTTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_6_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_6=850,22
PRIMER_RIGHT_6=1612,20
PRIMER_LEFT_6_TM=60.912
PRIMER_RIGHT_6_TM=59.978
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=9.1000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=763
PRIMER_PAIR_7_PENALTY=2.934433
PRIMER_LEFT_7_PENALTY=2.912092
PRIMER_RIGHT_7_PENALTY=0.022341
PRIMER_LEFT_7_SEQUENCE=CTTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_7_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_7=850,22
PRIMER_RIGHT_7=1613,20
PRIMER_LEFT_7_TM=60.912
PRIMER_RIGHT_7_TM=59.978
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=764
PRIMER_PAIR_8_PENALTY=3.285283
PRIMER_LEFT_8_PENALTY=1.517694
PRIMER_RIGHT_8_PENALTY=1.767588
PRIMER_LEFT_8_SEQUENCE=TGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_8_SEQUENCE=CTCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_8=852,20
PRIMER_RIGHT_8=1615,21
PRIMER_LEFT_8_TM=58.482
PRIMER_RIGHT_8_TM=59.232
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=764
PRIMER_PAIR_9_PENALTY=3.316821
PRIMER_LEFT_9_PENALTY=1.517694
PRIMER_RIGHT_9_PENALTY=1.799127
PRIMER_LEFT_9_SEQUENCE=TGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_9_SEQUENCE=TCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_9=852,20
PRIMER_RIGHT_9=1614,20
PRIMER_LEFT_9_TM=58.482
PRIMER_RIGHT_9_TM=58.201
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=763
PRIMER_PAIR_10_PENALTY=3.509559
PRIMER_LEFT_10_PENALTY=3.487218
PRIMER_RIGHT_10_PENALTY=0.022341
PRIMER_LEFT_10_SEQUENCE=GACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_10_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_10=744,23
PRIMER_RIGHT_10=1612,20
PRIMER_LEFT_10_TM=59.513
PRIMER_RIGHT_10_TM=59.978
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=869
PRIMER_PAIR_11_PENALTY=3.509559
PRIMER_LEFT_11_PENALTY=3.487218
PRIMER_RIGHT_11_PENALTY=0.022341
PRIMER_LEFT_11_SEQUENCE=GACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_11_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_11=744,23
PRIMER_RIGHT_11=1613,20
PRIMER_LEFT_11_TM=59.513
PRIMER_RIGHT_11_TM=59.978
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=870
PRIMER_PAIR_12_PENALTY=3.820271
PRIMER_LEFT_12_PENALTY=1.041745
PRIMER_RIGHT_12_PENALTY=2.778526
PRIMER_LEFT_12_SEQUENCE=TTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_12_SEQUENCE=TCCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_12=851,21
PRIMER_RIGHT_12=1614,21
PRIMER_LEFT_12_TM=59.958
PRIMER_RIGHT_12_TM=61.779
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=764
PRIMER_PAIR_13_PENALTY=3.839010
PRIMER_LEFT_13_PENALTY=3.816669
PRIMER_RIGHT_13_PENALTY=0.022341
PRIMER_LEFT_13_SEQUENCE=CCAGTCCTATTTTCTCACCAAAG
PRIMER_RIGHT_13_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_13=746,23
PRIMER_RIGHT_13=1612,20
PRIMER_LEFT_13_TM=59.183
PRIMER_RIGHT_13_TM=59.978
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=9.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=867
PRIMER_PAIR_14_PENALTY=3.839010
PRIMER_LEFT_14_PENALTY=3.816669
PRIMER_RIGHT_14_PENALTY=0.022341
PRIMER_LEFT_14_SEQUENCE=CCAGTCCTATTTTCTCACCAAAG
PRIMER_RIGHT_14_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_14=746,23
PRIMER_RIGHT_14=1613,20
PRIMER_LEFT_14_TM=59.183
PRIMER_RIGHT_14_TM=59.978
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=868
PRIMER_PAIR_15_PENALTY=3.848158
PRIMER_LEFT_15_PENALTY=3.825817
PRIMER_RIGHT_15_PENALTY=0.022341
PRIMER_LEFT_15_SEQUENCE=GACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_15_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_15=744,22
PRIMER_RIGHT_15=1612,20
PRIMER_LEFT_15_TM=58.174
PRIMER_RIGHT_15_TM=59.978
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=9.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=869
PRIMER_PAIR_16_PENALTY=3.848158
PRIMER_LEFT_16_PENALTY=3.825817
PRIMER_RIGHT_16_PENALTY=0.022341
PRIMER_LEFT_16_SEQUENCE=GACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_16_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_16=744,22
PRIMER_RIGHT_16=1613,20
PRIMER_LEFT_16_TM=58.174
PRIMER_RIGHT_16_TM=59.978
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=870
PRIMER_PAIR_17_PENALTY=3.865535
PRIMER_LEFT_17_PENALTY=3.843194
PRIMER_RIGHT_17_PENALTY=0.022341
PRIMER_LEFT_17_SEQUENCE=AGACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_17_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_17=743,23
PRIMER_RIGHT_17=1612,20
PRIMER_LEFT_17_TM=59.157
PRIMER_RIGHT_17_TM=59.978
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.1000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=870
PRIMER_PAIR_18_PENALTY=3.865535
PRIMER_LEFT_18_PENALTY=3.843194
PRIMER_RIGHT_18_PENALTY=0.022341
PRIMER_LEFT_18_SEQUENCE=AGACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_18_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_18=743,23
PRIMER_RIGHT_18=1613,20
PRIMER_LEFT_18_TM=59.157
PRIMER_RIGHT_18_TM=59.978
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=871
PRIMER_PAIR_19_PENALTY=4.066716
PRIMER_LEFT_19_PENALTY=1.041745
PRIMER_RIGHT_19_PENALTY=3.024972
PRIMER_LEFT_19_SEQUENCE=TTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_19_SEQUENCE=TCTCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_19=851,21
PRIMER_RIGHT_19=1616,22
PRIMER_LEFT_19_TM=59.958
PRIMER_RIGHT_19_TM=61.025
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=766
PRIMER_PAIR_20_PENALTY=4.087795
PRIMER_LEFT_20_PENALTY=4.065454
PRIMER_RIGHT_20_PENALTY=0.022341
PRIMER_LEFT_20_SEQUENCE=ACCAGTCCTATTTTCTCACCAAAG
PRIMER_RIGHT_20_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_20=745,24
PRIMER_RIGHT_20=1612,20
PRIMER_LEFT_20_TM=59.935
PRIMER_RIGHT_20_TM=59.978
PRIMER_LEFT_20_GC_PERCENT=41.667
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.3000
PRIMER_RIGHT_20_END_STABILITY=9.1000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=868
PRIMER_PAIR_21_PENALTY=4.087795
PRIMER_LEFT_21_PENALTY=4.065454
PRIMER_RIGHT_21_PENALTY=0.022341
PRIMER_LEFT_21_SEQUENCE=ACCAGTCCTATTTTCTCACCAAAG
PRIMER_RIGHT_21_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_21=745,24
PRIMER_RIGHT_21=1613,20
PRIMER_LEFT_21_TM=59.935
PRIMER_RIGHT_21_TM=59.978
PRIMER_LEFT_21_GC_PERCENT=41.667
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=869
PRIMER_PAIR_22_PENALTY=4.212851
PRIMER_LEFT_22_PENALTY=4.190510
PRIMER_RIGHT_22_PENALTY=0.022341
PRIMER_LEFT_22_SEQUENCE=CCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_22_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_22=746,21
PRIMER_RIGHT_22=1612,20
PRIMER_LEFT_22_TM=56.809
PRIMER_RIGHT_22_TM=59.978
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=9.1000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=867
PRIMER_PAIR_23_PENALTY=4.212851
PRIMER_LEFT_23_PENALTY=4.190510
PRIMER_RIGHT_23_PENALTY=0.022341
PRIMER_LEFT_23_SEQUENCE=CCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_23_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_23=746,21
PRIMER_RIGHT_23=1613,20
PRIMER_LEFT_23_TM=56.809
PRIMER_RIGHT_23_TM=59.978
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=868
PRIMER_PAIR_24_PENALTY=4.296220
PRIMER_LEFT_24_PENALTY=1.517694
PRIMER_RIGHT_24_PENALTY=2.778526
PRIMER_LEFT_24_SEQUENCE=TGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_24_SEQUENCE=TCCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_24=852,20
PRIMER_RIGHT_24=1614,21
PRIMER_LEFT_24_TM=58.482
PRIMER_RIGHT_24_TM=61.779
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=763
PRIMER_PAIR_25_PENALTY=4.298152
PRIMER_LEFT_25_PENALTY=4.275811
PRIMER_RIGHT_25_PENALTY=0.022341
PRIMER_LEFT_25_SEQUENCE=ACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_25_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_25=745,22
PRIMER_RIGHT_25=1612,20
PRIMER_LEFT_25_TM=57.724
PRIMER_RIGHT_25_TM=59.978
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=868
PRIMER_PAIR_26_PENALTY=4.298152
PRIMER_LEFT_26_PENALTY=4.275811
PRIMER_RIGHT_26_PENALTY=0.022341
PRIMER_LEFT_26_SEQUENCE=ACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_26_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_26=745,22
PRIMER_RIGHT_26=1613,20
PRIMER_LEFT_26_TM=57.724
PRIMER_RIGHT_26_TM=59.978
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=869
PRIMER_PAIR_27_PENALTY=4.413086
PRIMER_LEFT_27_PENALTY=4.390745
PRIMER_RIGHT_27_PENALTY=0.022341
PRIMER_LEFT_27_SEQUENCE=AGACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_27_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_27=743,24
PRIMER_RIGHT_27=1612,20
PRIMER_LEFT_27_TM=60.391
PRIMER_RIGHT_27_TM=59.978
PRIMER_LEFT_27_GC_PERCENT=41.667
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=9.1000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=870
PRIMER_PAIR_28_PENALTY=4.413086
PRIMER_LEFT_28_PENALTY=4.390745
PRIMER_RIGHT_28_PENALTY=0.022341
PRIMER_LEFT_28_SEQUENCE=AGACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_28_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_28=743,24
PRIMER_RIGHT_28=1613,20
PRIMER_LEFT_28_TM=60.391
PRIMER_RIGHT_28_TM=59.978
PRIMER_LEFT_28_GC_PERCENT=41.667
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=871
PRIMER_PAIR_29_PENALTY=4.542666
PRIMER_LEFT_29_PENALTY=1.517694
PRIMER_RIGHT_29_PENALTY=3.024972
PRIMER_LEFT_29_SEQUENCE=TGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_29_SEQUENCE=TCTCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_29=852,20
PRIMER_RIGHT_29=1616,22
PRIMER_LEFT_29_TM=58.482
PRIMER_RIGHT_29_TM=61.025
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=765
PRIMER_PAIR_30_PENALTY=4.679681
PRIMER_LEFT_30_PENALTY=2.912092
PRIMER_RIGHT_30_PENALTY=1.767588
PRIMER_LEFT_30_SEQUENCE=CTTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_30_SEQUENCE=CTCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_30=850,22
PRIMER_RIGHT_30=1615,21
PRIMER_LEFT_30_TM=60.912
PRIMER_RIGHT_30_TM=59.232
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=766
PRIMER_PAIR_31_PENALTY=4.689222
PRIMER_LEFT_31_PENALTY=4.666881
PRIMER_RIGHT_31_PENALTY=0.022341
PRIMER_LEFT_31_SEQUENCE=ACTTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_31_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_31=849,23
PRIMER_RIGHT_31=1612,20
PRIMER_LEFT_31_TM=61.667
PRIMER_RIGHT_31_TM=59.978
PRIMER_LEFT_31_GC_PERCENT=47.826
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=9.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=764
PRIMER_PAIR_32_PENALTY=4.689222
PRIMER_LEFT_32_PENALTY=4.666881
PRIMER_RIGHT_32_PENALTY=0.022341
PRIMER_LEFT_32_SEQUENCE=ACTTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_32_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_32=849,23
PRIMER_RIGHT_32=1613,20
PRIMER_LEFT_32_TM=61.667
PRIMER_RIGHT_32_TM=59.978
PRIMER_LEFT_32_GC_PERCENT=47.826
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=765
PRIMER_PAIR_33_PENALTY=4.705329
PRIMER_LEFT_33_PENALTY=3.487218
PRIMER_RIGHT_33_PENALTY=1.218111
PRIMER_LEFT_33_SEQUENCE=GACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_33_SEQUENCE=GGATGGATTGAAGTGGAACAA
PRIMER_LEFT_33=744,23
PRIMER_RIGHT_33=1364,21
PRIMER_LEFT_33_TM=59.513
PRIMER_RIGHT_33_TM=59.782
PRIMER_LEFT_33_GC_PERCENT=43.478
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=621
PRIMER_PAIR_34_PENALTY=4.711219
PRIMER_LEFT_34_PENALTY=2.912092
PRIMER_RIGHT_34_PENALTY=1.799127
PRIMER_LEFT_34_SEQUENCE=CTTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_34_SEQUENCE=TCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_34=850,22
PRIMER_RIGHT_34=1614,20
PRIMER_LEFT_34_TM=60.912
PRIMER_RIGHT_34_TM=58.201
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=765
PRIMER_PAIR_35_PENALTY=4.777603
PRIMER_LEFT_35_PENALTY=4.755263
PRIMER_RIGHT_35_PENALTY=0.022341
PRIMER_LEFT_35_SEQUENCE=ACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_35_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_35=745,21
PRIMER_RIGHT_35=1612,20
PRIMER_LEFT_35_TM=56.245
PRIMER_RIGHT_35_TM=59.978
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=9.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=868
PRIMER_PAIR_36_PENALTY=4.777603
PRIMER_LEFT_36_PENALTY=4.755263
PRIMER_RIGHT_36_PENALTY=0.022341
PRIMER_LEFT_36_SEQUENCE=ACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_36_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_36=745,21
PRIMER_RIGHT_36=1613,20
PRIMER_LEFT_36_TM=56.245
PRIMER_RIGHT_36_TM=59.978
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=869
PRIMER_PAIR_37_PENALTY=4.803404
PRIMER_LEFT_37_PENALTY=1.041745
PRIMER_RIGHT_37_PENALTY=3.761659
PRIMER_LEFT_37_SEQUENCE=TTGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_37_SEQUENCE=TGTCCTTTGGTGAGAAAATAGG
PRIMER_LEFT_37=851,21
PRIMER_RIGHT_37=1810,22
PRIMER_LEFT_37_TM=59.958
PRIMER_RIGHT_37_TM=58.238
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.1000
PRIMER_RIGHT_37_END_STABILITY=7.1000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=960
PRIMER_PAIR_38_PENALTY=4.806665
PRIMER_LEFT_38_PENALTY=4.784324
PRIMER_RIGHT_38_PENALTY=0.022341
PRIMER_LEFT_38_SEQUENCE=CCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_38_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_38=746,20
PRIMER_RIGHT_38=1612,20
PRIMER_LEFT_38_TM=55.216
PRIMER_RIGHT_38_TM=59.978
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=9.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=867
PRIMER_PAIR_39_PENALTY=4.806665
PRIMER_LEFT_39_PENALTY=4.784324
PRIMER_RIGHT_39_PENALTY=0.022341
PRIMER_LEFT_39_SEQUENCE=CCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_39_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_39=746,20
PRIMER_RIGHT_39=1613,20
PRIMER_LEFT_39_TM=55.216
PRIMER_RIGHT_39_TM=59.978
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=868
PRIMER_PAIR_40_PENALTY=4.826250
PRIMER_LEFT_40_PENALTY=4.803909
PRIMER_RIGHT_40_PENALTY=0.022341
PRIMER_LEFT_40_SEQUENCE=TAGACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_40_SEQUENCE=CTATCTGCTCGCAAAGTCCC
PRIMER_LEFT_40=742,24
PRIMER_RIGHT_40=1612,20
PRIMER_LEFT_40_TM=59.196
PRIMER_RIGHT_40_TM=59.978
PRIMER_LEFT_40_GC_PERCENT=41.667
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=9.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=871
PRIMER_PAIR_41_PENALTY=4.826250
PRIMER_LEFT_41_PENALTY=4.803909
PRIMER_RIGHT_41_PENALTY=0.022341
PRIMER_LEFT_41_SEQUENCE=TAGACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_41_SEQUENCE=CCTATCTGCTCGCAAAGTCC
PRIMER_LEFT_41=742,24
PRIMER_RIGHT_41=1613,20
PRIMER_LEFT_41_TM=59.196
PRIMER_RIGHT_41_TM=59.978
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=872
PRIMER_PAIR_42_PENALTY=5.034780
PRIMER_LEFT_42_PENALTY=3.816669
PRIMER_RIGHT_42_PENALTY=1.218111
PRIMER_LEFT_42_SEQUENCE=CCAGTCCTATTTTCTCACCAAAG
PRIMER_RIGHT_42_SEQUENCE=GGATGGATTGAAGTGGAACAA
PRIMER_LEFT_42=746,23
PRIMER_RIGHT_42=1364,21
PRIMER_LEFT_42_TM=59.183
PRIMER_RIGHT_42_TM=59.782
PRIMER_LEFT_42_GC_PERCENT=43.478
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=619
PRIMER_PAIR_43_PENALTY=5.043928
PRIMER_LEFT_43_PENALTY=3.825817
PRIMER_RIGHT_43_PENALTY=1.218111
PRIMER_LEFT_43_SEQUENCE=GACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_43_SEQUENCE=GGATGGATTGAAGTGGAACAA
PRIMER_LEFT_43=744,22
PRIMER_RIGHT_43=1364,21
PRIMER_LEFT_43_TM=58.174
PRIMER_RIGHT_43_TM=59.782
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=621
PRIMER_PAIR_44_PENALTY=5.061305
PRIMER_LEFT_44_PENALTY=3.843194
PRIMER_RIGHT_44_PENALTY=1.218111
PRIMER_LEFT_44_SEQUENCE=AGACCAGTCCTATTTTCTCACCA
PRIMER_RIGHT_44_SEQUENCE=GGATGGATTGAAGTGGAACAA
PRIMER_LEFT_44=743,23
PRIMER_RIGHT_44=1364,21
PRIMER_LEFT_44_TM=59.157
PRIMER_RIGHT_44_TM=59.782
PRIMER_LEFT_44_GC_PERCENT=43.478
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=622
PRIMER_PAIR_45_PENALTY=5.156174
PRIMER_LEFT_45_PENALTY=3.487218
PRIMER_RIGHT_45_PENALTY=1.668956
PRIMER_LEFT_45_SEQUENCE=GACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_45_SEQUENCE=GGATGGATTGAAGTGGAACA
PRIMER_LEFT_45=744,23
PRIMER_RIGHT_45=1364,20
PRIMER_LEFT_45_TM=59.513
PRIMER_RIGHT_45_TM=58.331
PRIMER_LEFT_45_GC_PERCENT=43.478
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=621
PRIMER_PAIR_46_PENALTY=5.254806
PRIMER_LEFT_46_PENALTY=3.487218
PRIMER_RIGHT_46_PENALTY=1.767588
PRIMER_LEFT_46_SEQUENCE=GACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_46_SEQUENCE=CTCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_46=744,23
PRIMER_RIGHT_46=1615,21
PRIMER_LEFT_46_TM=59.513
PRIMER_RIGHT_46_TM=59.232
PRIMER_LEFT_46_GC_PERCENT=43.478
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=872
PRIMER_PAIR_47_PENALTY=5.279353
PRIMER_LEFT_47_PENALTY=1.517694
PRIMER_RIGHT_47_PENALTY=3.761659
PRIMER_LEFT_47_SEQUENCE=TGTTCCACTTCAGTCCATCC
PRIMER_RIGHT_47_SEQUENCE=TGTCCTTTGGTGAGAAAATAGG
PRIMER_LEFT_47=852,20
PRIMER_RIGHT_47=1810,22
PRIMER_LEFT_47_TM=58.482
PRIMER_RIGHT_47_TM=58.238
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=7.1000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=959
PRIMER_PAIR_48_PENALTY=5.283565
PRIMER_LEFT_48_PENALTY=4.065454
PRIMER_RIGHT_48_PENALTY=1.218111
PRIMER_LEFT_48_SEQUENCE=ACCAGTCCTATTTTCTCACCAAAG
PRIMER_RIGHT_48_SEQUENCE=GGATGGATTGAAGTGGAACAA
PRIMER_LEFT_48=745,24
PRIMER_RIGHT_48=1364,21
PRIMER_LEFT_48_TM=59.935
PRIMER_RIGHT_48_TM=59.782
PRIMER_LEFT_48_GC_PERCENT=41.667
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=620
PRIMER_PAIR_49_PENALTY=5.286344
PRIMER_LEFT_49_PENALTY=3.487218
PRIMER_RIGHT_49_PENALTY=1.799127
PRIMER_LEFT_49_SEQUENCE=GACCAGTCCTATTTTCTCACCAA
PRIMER_RIGHT_49_SEQUENCE=TCCTATCTGCTCGCAAAGTC
PRIMER_LEFT_49=744,23
PRIMER_RIGHT_49=1614,20
PRIMER_LEFT_49_TM=59.513
PRIMER_RIGHT_49_TM=58.201
PRIMER_LEFT_49_GC_PERCENT=43.478
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=871
=
SEQUENCE_ID=MAGIC.338_2_5934464_5934506_mt-0_ler-0_5255_271625_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=NNNNNNNGTNNNNNNNNNNNNNNNNNNACCANTGGTTTGGAGGAGANGGTTNCATCTTGGAGTCANTNGAAACATTACTNAAGTATCTCCCGGCATTANGNGGATTTGATAACTTGAGACCTTGTGGANANATTTGGTTATGATAGTCTCTTCCAAATCGAATAATCGNNGGACATGGATTCANANNTGTNTTCCTCTCNACTTTGTTNTATATTTGTAAGACNTCNGCAAGGCCCTGNCNATACACCGCAAAACCTCCGCTCNGCATTCTCCGACTTAGATACATNNTCAATCNGTTTTATNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNATTCAGTTTNTGTGTGANATTGGTTAAGCGTNTGNTTAAGATANAAGGCTTTTANGTGTAGTAANGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAAATNNNNNNNNNNNNNNNNAACAGAATTTACTTCAAAANGCNATTGAATTAAATAGGAAANCNTCNTTAAAAAATTNCATATTGNAANNTGGANNTATNNNNNNNNNNNATATTAGAATAAATAATTTTCAGCATNTTCNTCAAAGATTTTTTTAAAATAAAAATTAAATAAATTGACATTTTCTTCCTTTCTGGACATAAACCTAAATTAAGGAACATAATATTGAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAATNGAAAGTCATTAAATATGAAACCGATCCACAACTCAACAGAAGAACAATACACANCTGTTTATAGGTTTTCACAATAAACCAAAAGTAACCAACTAGACCGGAAAAAACTTAGACATGGAAACATAAAATGCAAAGCTTCTTTGTTTACTAATCAAAATTCATGAACTCACGAACTGCGATTTCTGCAGTTGCATTCGAGAAACAGGAGTACACCTAAGAGGATGAGCCATGTTTAAGGTAATTCCAGCAACAGTCTCTTCCATGTTGACCTTATCTCCATTGGTTCTCCAGTCAAAGCAATGCACCATCATTCCTATTGCGGTTCCTACAAATATATAACCTAGATTTACNCCAGGACATCCTCTTCTTCCGCTGCCAAAAGGAATGTACTTAAGTTCTTGCTCTCTCTCCTCTTCTTTTCCTCTTGAAGAANCTAGAAACCTCTCTGGCTTAAACTCATCAGGATCTTCCCAAGAATCGGGATCTCTCATCACGGCATAAGCATTAACAACAAGTGTTGTGTTCTTTGGTACGTAACAGCCTCCAATCGTACATCCATCTGTGACTTTCCTTCCCAAGAGAGGTGCTGGTGGGTGTAATCTTAGCCCTTCCTTGATGACCGCTTGCAAATAAGGGAGGTTCGGTAGATCCTTCTCTTGAATCAACCTTGTTTTGCCTACAACAGAATCGATTTCTTTTCTCAATCTCTCAAGAATGTTAGGCTTCTTAATGATCTTTGCCATTGTCCACTCGATTGTTTGCGCTGAGGTGTCAGTGCCTCCAAGGATAAGCTCCTGTAATNAAAAAAAACACAGGACAATTAATGTTGTTGTATTGGATTTCTCAATGAATCCACCAAGTGAAAGGAGAATTTACCACGAACAAGGACTTGAGGTGGTCCCTAGTGATCTTATACTCTGCCTTTCCATCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=5.085619
PRIMER_LEFT_0_PENALTY=5.057323
PRIMER_RIGHT_0_PENALTY=0.028296
PRIMER_LEFT_0_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_0_SEQUENCE=TGCTTATGCCGTGATGAGAG
PRIMER_LEFT_0=722,22
PRIMER_RIGHT_0=1614,20
PRIMER_LEFT_0_TM=56.943
PRIMER_RIGHT_0_TM=59.972
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=893
PRIMER_PAIR_1_PENALTY=5.137725
PRIMER_LEFT_1_PENALTY=5.057323
PRIMER_RIGHT_1_PENALTY=0.080402
PRIMER_LEFT_1_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_1_SEQUENCE=ATGATGGTGCATTGCTTTGA
PRIMER_LEFT_1=722,22
PRIMER_RIGHT_1=1421,20
PRIMER_LEFT_1_TM=56.943
PRIMER_RIGHT_1_TM=60.080
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=700
PRIMER_PAIR_2_PENALTY=5.206718
PRIMER_LEFT_2_PENALTY=5.057323
PRIMER_RIGHT_2_PENALTY=0.149395
PRIMER_LEFT_2_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_2_SEQUENCE=ATTACACCCACCAGCACCTC
PRIMER_LEFT_2=722,22
PRIMER_RIGHT_2=1710,20
PRIMER_LEFT_2_TM=56.943
PRIMER_RIGHT_2_TM=59.851
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=989
PRIMER_PAIR_3_PENALTY=5.206718
PRIMER_LEFT_3_PENALTY=5.057323
PRIMER_RIGHT_3_PENALTY=0.149395
PRIMER_LEFT_3_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_3_SEQUENCE=GATTACACCCACCAGCACCT
PRIMER_LEFT_3=722,22
PRIMER_RIGHT_3=1711,20
PRIMER_LEFT_3_TM=56.943
PRIMER_RIGHT_3_TM=59.851
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=990
PRIMER_PAIR_4_PENALTY=5.206718
PRIMER_LEFT_4_PENALTY=5.057323
PRIMER_RIGHT_4_PENALTY=0.149395
PRIMER_LEFT_4_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_4_SEQUENCE=AGATTACACCCACCAGCACC
PRIMER_LEFT_4=722,22
PRIMER_RIGHT_4=1712,20
PRIMER_LEFT_4_TM=56.943
PRIMER_RIGHT_4_TM=59.851
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=991
PRIMER_PAIR_5_PENALTY=5.207093
PRIMER_LEFT_5_PENALTY=5.057323
PRIMER_RIGHT_5_PENALTY=0.149770
PRIMER_LEFT_5_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_5_SEQUENCE=TGAGAGATCCCGATTCTTGG
PRIMER_LEFT_5=722,22
PRIMER_RIGHT_5=1600,20
PRIMER_LEFT_5_TM=56.943
PRIMER_RIGHT_5_TM=60.150
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=879
PRIMER_PAIR_6_PENALTY=5.272873
PRIMER_LEFT_6_PENALTY=5.057323
PRIMER_RIGHT_6_PENALTY=0.215550
PRIMER_LEFT_6_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_6_SEQUENCE=AACCGCAATAGGAATGATGG
PRIMER_LEFT_6=722,22
PRIMER_RIGHT_6=1434,20
PRIMER_LEFT_6_TM=56.943
PRIMER_RIGHT_6_TM=59.784
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=713
PRIMER_PAIR_7_PENALTY=5.335447
PRIMER_LEFT_7_PENALTY=5.057323
PRIMER_RIGHT_7_PENALTY=0.278124
PRIMER_LEFT_7_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_7_SEQUENCE=ATGGTGCATTGCTTTGACTG
PRIMER_LEFT_7=722,22
PRIMER_RIGHT_7=1418,20
PRIMER_LEFT_7_TM=56.943
PRIMER_RIGHT_7_TM=59.722
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=697
PRIMER_PAIR_8_PENALTY=5.381598
PRIMER_LEFT_8_PENALTY=5.057323
PRIMER_RIGHT_8_PENALTY=0.324275
PRIMER_LEFT_8_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_8_SEQUENCE=TCTGTTGAGTTGTGGATCGG
PRIMER_LEFT_8=722,22
PRIMER_RIGHT_8=1150,20
PRIMER_LEFT_8_TM=56.943
PRIMER_RIGHT_8_TM=59.676
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=9.8000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=429
PRIMER_PAIR_9_PENALTY=5.466742
PRIMER_LEFT_9_PENALTY=5.057323
PRIMER_RIGHT_9_PENALTY=0.409420
PRIMER_LEFT_9_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_9_SEQUENCE=CAGAAATCGCAGTTCGTGAG
PRIMER_LEFT_9=722,22
PRIMER_RIGHT_9=1295,20
PRIMER_LEFT_9_TM=56.943
PRIMER_RIGHT_9_TM=59.591
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=574
PRIMER_PAIR_10_PENALTY=5.511926
PRIMER_LEFT_10_PENALTY=5.057323
PRIMER_RIGHT_10_PENALTY=0.454603
PRIMER_LEFT_10_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_10_SEQUENCE=TGGAAGAGACTGTTGCTGGA
PRIMER_LEFT_10=722,22
PRIMER_RIGHT_10=1372,20
PRIMER_LEFT_10_TM=56.943
PRIMER_RIGHT_10_TM=59.545
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=8.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=651
PRIMER_PAIR_11_PENALTY=5.514002
PRIMER_LEFT_11_PENALTY=5.433599
PRIMER_RIGHT_11_PENALTY=0.080402
PRIMER_LEFT_11_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_11_SEQUENCE=ATGATGGTGCATTGCTTTGA
PRIMER_LEFT_11=730,22
PRIMER_RIGHT_11=1421,20
PRIMER_LEFT_11_TM=56.566
PRIMER_RIGHT_11_TM=60.080
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=692
PRIMER_PAIR_12_PENALTY=5.529375
PRIMER_LEFT_12_PENALTY=5.057323
PRIMER_RIGHT_12_PENALTY=0.472052
PRIMER_LEFT_12_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_12_SEQUENCE=AGGAACCGCAATAGGAATGA
PRIMER_LEFT_12=722,22
PRIMER_RIGHT_12=1437,20
PRIMER_LEFT_12_TM=56.943
PRIMER_RIGHT_12_TM=59.528
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=716
PRIMER_PAIR_13_PENALTY=5.539985
PRIMER_LEFT_13_PENALTY=5.057323
PRIMER_RIGHT_13_PENALTY=0.482662
PRIMER_LEFT_13_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_13_SEQUENCE=GGATGTACGATTGGAGGCTG
PRIMER_LEFT_13=722,22
PRIMER_RIGHT_13=1667,20
PRIMER_LEFT_13_TM=56.943
PRIMER_RIGHT_13_TM=60.483
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=946
PRIMER_PAIR_14_PENALTY=5.567962
PRIMER_LEFT_14_PENALTY=5.433599
PRIMER_RIGHT_14_PENALTY=0.134363
PRIMER_LEFT_14_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_14_SEQUENCE=CTCGAATGCAACTGCAGAAA
PRIMER_LEFT_14=730,22
PRIMER_RIGHT_14=1309,20
PRIMER_LEFT_14_TM=56.566
PRIMER_RIGHT_14_TM=60.134
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=580
PRIMER_PAIR_15_PENALTY=5.582994
PRIMER_LEFT_15_PENALTY=5.433599
PRIMER_RIGHT_15_PENALTY=0.149395
PRIMER_LEFT_15_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_15_SEQUENCE=ATTACACCCACCAGCACCTC
PRIMER_LEFT_15=730,22
PRIMER_RIGHT_15=1710,20
PRIMER_LEFT_15_TM=56.566
PRIMER_RIGHT_15_TM=59.851
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=981
PRIMER_PAIR_16_PENALTY=5.582994
PRIMER_LEFT_16_PENALTY=5.433599
PRIMER_RIGHT_16_PENALTY=0.149395
PRIMER_LEFT_16_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_16_SEQUENCE=GATTACACCCACCAGCACCT
PRIMER_LEFT_16=730,22
PRIMER_RIGHT_16=1711,20
PRIMER_LEFT_16_TM=56.566
PRIMER_RIGHT_16_TM=59.851
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=982
PRIMER_PAIR_17_PENALTY=5.582994
PRIMER_LEFT_17_PENALTY=5.433599
PRIMER_RIGHT_17_PENALTY=0.149395
PRIMER_LEFT_17_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_17_SEQUENCE=AGATTACACCCACCAGCACC
PRIMER_LEFT_17=730,22
PRIMER_RIGHT_17=1712,20
PRIMER_LEFT_17_TM=56.566
PRIMER_RIGHT_17_TM=59.851
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=983
PRIMER_PAIR_18_PENALTY=5.583370
PRIMER_LEFT_18_PENALTY=5.433599
PRIMER_RIGHT_18_PENALTY=0.149770
PRIMER_LEFT_18_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_18_SEQUENCE=TGAGAGATCCCGATTCTTGG
PRIMER_LEFT_18=730,22
PRIMER_RIGHT_18=1600,20
PRIMER_LEFT_18_TM=56.566
PRIMER_RIGHT_18_TM=60.150
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=871
PRIMER_PAIR_19_PENALTY=5.596638
PRIMER_LEFT_19_PENALTY=5.057323
PRIMER_RIGHT_19_PENALTY=0.539315
PRIMER_LEFT_19_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_19_SEQUENCE=TGCATTGCTTTGACTGGAGA
PRIMER_LEFT_19=722,22
PRIMER_RIGHT_19=1414,20
PRIMER_LEFT_19_TM=56.943
PRIMER_RIGHT_19_TM=60.539
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=693
PRIMER_PAIR_20_PENALTY=5.624430
PRIMER_LEFT_20_PENALTY=5.057323
PRIMER_RIGHT_20_PENALTY=0.567107
PRIMER_LEFT_20_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_20_SEQUENCE=TTACACCCACCAGCACCTCT
PRIMER_LEFT_20=722,22
PRIMER_RIGHT_20=1709,20
PRIMER_LEFT_20_TM=56.943
PRIMER_RIGHT_20_TM=60.567
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=988
PRIMER_PAIR_21_PENALTY=5.642539
PRIMER_LEFT_21_PENALTY=5.057323
PRIMER_RIGHT_21_PENALTY=0.585216
PRIMER_LEFT_21_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_21_SEQUENCE=TGCTTTGACTGGAGAACCAA
PRIMER_LEFT_21=722,22
PRIMER_RIGHT_21=1409,20
PRIMER_LEFT_21_TM=56.943
PRIMER_RIGHT_21_TM=59.415
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=688
PRIMER_PAIR_22_PENALTY=5.649150
PRIMER_LEFT_22_PENALTY=5.433599
PRIMER_RIGHT_22_PENALTY=0.215550
PRIMER_LEFT_22_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_22_SEQUENCE=AACCGCAATAGGAATGATGG
PRIMER_LEFT_22=730,22
PRIMER_RIGHT_22=1434,20
PRIMER_LEFT_22_TM=56.566
PRIMER_RIGHT_22_TM=59.784
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=705
PRIMER_PAIR_23_PENALTY=5.711724
PRIMER_LEFT_23_PENALTY=5.433599
PRIMER_RIGHT_23_PENALTY=0.278124
PRIMER_LEFT_23_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_23_SEQUENCE=ATGGTGCATTGCTTTGACTG
PRIMER_LEFT_23=730,22
PRIMER_RIGHT_23=1418,20
PRIMER_LEFT_23_TM=56.566
PRIMER_RIGHT_23_TM=59.722
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=689
PRIMER_PAIR_24_PENALTY=5.757875
PRIMER_LEFT_24_PENALTY=5.433599
PRIMER_RIGHT_24_PENALTY=0.324275
PRIMER_LEFT_24_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_24_SEQUENCE=TCTGTTGAGTTGTGGATCGG
PRIMER_LEFT_24=730,22
PRIMER_RIGHT_24=1150,20
PRIMER_LEFT_24_TM=56.566
PRIMER_RIGHT_24_TM=59.676
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=9.8000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=421
PRIMER_PAIR_25_PENALTY=5.784627
PRIMER_LEFT_25_PENALTY=5.057323
PRIMER_RIGHT_25_PENALTY=0.727304
PRIMER_LEFT_25_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_25_SEQUENCE=ACCGCAATAGGAATGATGGT
PRIMER_LEFT_25=722,22
PRIMER_RIGHT_25=1433,20
PRIMER_LEFT_25_TM=56.943
PRIMER_RIGHT_25_TM=59.273
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=7.8000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=712
PRIMER_PAIR_26_PENALTY=5.802352
PRIMER_LEFT_26_PENALTY=5.057323
PRIMER_RIGHT_26_PENALTY=0.745029
PRIMER_LEFT_26_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_26_SEQUENCE=CACCTCTCTTGGGAAGGAAA
PRIMER_LEFT_26=722,22
PRIMER_RIGHT_26=1696,20
PRIMER_LEFT_26_TM=56.943
PRIMER_RIGHT_26_TM=59.255
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=975
PRIMER_PAIR_27_PENALTY=5.843019
PRIMER_LEFT_27_PENALTY=5.433599
PRIMER_RIGHT_27_PENALTY=0.409420
PRIMER_LEFT_27_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_27_SEQUENCE=CAGAAATCGCAGTTCGTGAG
PRIMER_LEFT_27=730,22
PRIMER_RIGHT_27=1295,20
PRIMER_LEFT_27_TM=56.566
PRIMER_RIGHT_27_TM=59.591
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=566
PRIMER_PAIR_28_PENALTY=5.888202
PRIMER_LEFT_28_PENALTY=5.433599
PRIMER_RIGHT_28_PENALTY=0.454603
PRIMER_LEFT_28_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_28_SEQUENCE=TGGAAGAGACTGTTGCTGGA
PRIMER_LEFT_28=730,22
PRIMER_RIGHT_28=1372,20
PRIMER_LEFT_28_TM=56.566
PRIMER_RIGHT_28_TM=59.545
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=643
PRIMER_PAIR_29_PENALTY=5.905652
PRIMER_LEFT_29_PENALTY=5.433599
PRIMER_RIGHT_29_PENALTY=0.472052
PRIMER_LEFT_29_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_29_SEQUENCE=AGGAACCGCAATAGGAATGA
PRIMER_LEFT_29=730,22
PRIMER_RIGHT_29=1437,20
PRIMER_LEFT_29_TM=56.566
PRIMER_RIGHT_29_TM=59.528
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=708
PRIMER_PAIR_30_PENALTY=5.916261
PRIMER_LEFT_30_PENALTY=5.433599
PRIMER_RIGHT_30_PENALTY=0.482662
PRIMER_LEFT_30_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_30_SEQUENCE=GGATGTACGATTGGAGGCTG
PRIMER_LEFT_30=730,22
PRIMER_RIGHT_30=1667,20
PRIMER_LEFT_30_TM=56.566
PRIMER_RIGHT_30_TM=60.483
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=938
PRIMER_PAIR_31_PENALTY=5.931579
PRIMER_LEFT_31_PENALTY=5.057323
PRIMER_RIGHT_31_PENALTY=0.874256
PRIMER_LEFT_31_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_31_SEQUENCE=GGAATGATGGTGCATTGCTT
PRIMER_LEFT_31=722,22
PRIMER_RIGHT_31=1424,20
PRIMER_LEFT_31_TM=56.943
PRIMER_RIGHT_31_TM=60.874
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=703
PRIMER_PAIR_32_PENALTY=5.972915
PRIMER_LEFT_32_PENALTY=5.433599
PRIMER_RIGHT_32_PENALTY=0.539315
PRIMER_LEFT_32_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_32_SEQUENCE=TGCATTGCTTTGACTGGAGA
PRIMER_LEFT_32=730,22
PRIMER_RIGHT_32=1414,20
PRIMER_LEFT_32_TM=56.566
PRIMER_RIGHT_32_TM=60.539
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=685
PRIMER_PAIR_33_PENALTY=6.000706
PRIMER_LEFT_33_PENALTY=5.433599
PRIMER_RIGHT_33_PENALTY=0.567107
PRIMER_LEFT_33_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_33_SEQUENCE=TTACACCCACCAGCACCTCT
PRIMER_LEFT_33=730,22
PRIMER_RIGHT_33=1709,20
PRIMER_LEFT_33_TM=56.566
PRIMER_RIGHT_33_TM=60.567
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=980
PRIMER_PAIR_34_PENALTY=6.018815
PRIMER_LEFT_34_PENALTY=5.433599
PRIMER_RIGHT_34_PENALTY=0.585216
PRIMER_LEFT_34_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_34_SEQUENCE=TGCTTTGACTGGAGAACCAA
PRIMER_LEFT_34=730,22
PRIMER_RIGHT_34=1409,20
PRIMER_LEFT_34_TM=56.566
PRIMER_RIGHT_34_TM=59.415
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=680
PRIMER_PAIR_35_PENALTY=6.055488
PRIMER_LEFT_35_PENALTY=5.057323
PRIMER_RIGHT_35_PENALTY=0.998165
PRIMER_LEFT_35_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_35_SEQUENCE=GCATTGCTTTGACTGGAGAA
PRIMER_LEFT_35=722,22
PRIMER_RIGHT_35=1413,20
PRIMER_LEFT_35_TM=56.943
PRIMER_RIGHT_35_TM=59.002
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=692
PRIMER_PAIR_36_PENALTY=6.073688
PRIMER_LEFT_36_PENALTY=5.057323
PRIMER_RIGHT_36_PENALTY=1.016365
PRIMER_LEFT_36_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_36_SEQUENCE=TGTTGAGTTGTGGATCGGTT
PRIMER_LEFT_36=722,22
PRIMER_RIGHT_36=1148,20
PRIMER_LEFT_36_TM=56.943
PRIMER_RIGHT_36_TM=58.984
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=9.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=427
PRIMER_PAIR_37_PENALTY=6.077612
PRIMER_LEFT_37_PENALTY=5.057323
PRIMER_RIGHT_37_PENALTY=1.020289
PRIMER_LEFT_37_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_37_SEQUENCE=CTGTTGAGTTGTGGATCGGTT
PRIMER_LEFT_37=722,22
PRIMER_RIGHT_37=1149,21
PRIMER_LEFT_37_TM=56.943
PRIMER_RIGHT_37_TM=60.020
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=428
PRIMER_PAIR_38_PENALTY=6.078339
PRIMER_LEFT_38_PENALTY=5.057323
PRIMER_RIGHT_38_PENALTY=1.021016
PRIMER_LEFT_38_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_38_SEQUENCE=GCTTATGCCGTGATGAGAGA
PRIMER_LEFT_38=722,22
PRIMER_RIGHT_38=1613,20
PRIMER_LEFT_38_TM=56.943
PRIMER_RIGHT_38_TM=58.979
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=892
PRIMER_PAIR_39_PENALTY=6.153215
PRIMER_LEFT_39_PENALTY=5.057323
PRIMER_RIGHT_39_PENALTY=1.095892
PRIMER_LEFT_39_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_39_SEQUENCE=TTGACTGGAGAACCAATGGAG
PRIMER_LEFT_39=722,22
PRIMER_RIGHT_39=1405,21
PRIMER_LEFT_39_TM=56.943
PRIMER_RIGHT_39_TM=60.096
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=684
PRIMER_PAIR_40_PENALTY=6.160903
PRIMER_LEFT_40_PENALTY=5.433599
PRIMER_RIGHT_40_PENALTY=0.727304
PRIMER_LEFT_40_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_40_SEQUENCE=ACCGCAATAGGAATGATGGT
PRIMER_LEFT_40=730,22
PRIMER_RIGHT_40=1433,20
PRIMER_LEFT_40_TM=56.566
PRIMER_RIGHT_40_TM=59.273
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.8000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=704
PRIMER_PAIR_41_PENALTY=6.178629
PRIMER_LEFT_41_PENALTY=5.433599
PRIMER_RIGHT_41_PENALTY=0.745029
PRIMER_LEFT_41_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_41_SEQUENCE=CACCTCTCTTGGGAAGGAAA
PRIMER_LEFT_41=730,22
PRIMER_RIGHT_41=1696,20
PRIMER_LEFT_41_TM=56.566
PRIMER_RIGHT_41_TM=59.255
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=967
PRIMER_PAIR_42_PENALTY=6.183516
PRIMER_LEFT_42_PENALTY=5.057323
PRIMER_RIGHT_42_PENALTY=1.126193
PRIMER_LEFT_42_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_42_SEQUENCE=GCATTGCTTTGACTGGAGAAC
PRIMER_LEFT_42=722,22
PRIMER_RIGHT_42=1413,21
PRIMER_LEFT_42_TM=56.943
PRIMER_RIGHT_42_TM=59.874
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=692
PRIMER_PAIR_43_PENALTY=6.190041
PRIMER_LEFT_43_PENALTY=5.057323
PRIMER_RIGHT_43_PENALTY=1.132718
PRIMER_LEFT_43_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_43_SEQUENCE=TCCTGTTTCTCGAATGCAACT
PRIMER_LEFT_43=722,22
PRIMER_RIGHT_43=1317,21
PRIMER_LEFT_43_TM=56.943
PRIMER_RIGHT_43_TM=59.867
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=596
PRIMER_PAIR_44_PENALTY=6.191283
PRIMER_LEFT_44_PENALTY=5.057323
PRIMER_RIGHT_44_PENALTY=1.133960
PRIMER_LEFT_44_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_44_SEQUENCE=TACACCCACCAGCACCTCTC
PRIMER_LEFT_44=722,22
PRIMER_RIGHT_44=1708,20
PRIMER_LEFT_44_TM=56.943
PRIMER_RIGHT_44_TM=61.134
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=60.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=987
PRIMER_PAIR_45_PENALTY=6.198277
PRIMER_LEFT_45_PENALTY=5.057323
PRIMER_RIGHT_45_PENALTY=1.140954
PRIMER_LEFT_45_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_45_SEQUENCE=ATGGAAGAGACTGTTGCTGGA
PRIMER_LEFT_45=722,22
PRIMER_RIGHT_45=1373,21
PRIMER_LEFT_45_TM=56.943
PRIMER_RIGHT_45_TM=59.859
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=8.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=652
PRIMER_PAIR_46_PENALTY=6.307334
PRIMER_LEFT_46_PENALTY=5.057323
PRIMER_RIGHT_46_PENALTY=1.250011
PRIMER_LEFT_46_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_46_SEQUENCE=ATGCTTATGCCGTGATGAGAG
PRIMER_LEFT_46=722,22
PRIMER_RIGHT_46=1615,21
PRIMER_LEFT_46_TM=56.943
PRIMER_RIGHT_46_TM=60.250
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=894
PRIMER_PAIR_47_PENALTY=6.307855
PRIMER_LEFT_47_PENALTY=5.433599
PRIMER_RIGHT_47_PENALTY=0.874256
PRIMER_LEFT_47_SEQUENCE=CTTCCTTTCTGGACATAAACCT
PRIMER_RIGHT_47_SEQUENCE=GGAATGATGGTGCATTGCTT
PRIMER_LEFT_47=730,22
PRIMER_RIGHT_47=1424,20
PRIMER_LEFT_47_TM=56.566
PRIMER_RIGHT_47_TM=60.874
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=695
PRIMER_PAIR_48_PENALTY=6.331159
PRIMER_LEFT_48_PENALTY=5.057323
PRIMER_RIGHT_48_PENALTY=1.273836
PRIMER_LEFT_48_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_48_SEQUENCE=GATGGTGCATTGCTTTGACT
PRIMER_LEFT_48=722,22
PRIMER_RIGHT_48=1419,20
PRIMER_LEFT_48_TM=56.943
PRIMER_RIGHT_48_TM=58.726
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=698
PRIMER_PAIR_49_PENALTY=6.331587
PRIMER_LEFT_49_PENALTY=5.057323
PRIMER_RIGHT_49_PENALTY=1.274264
PRIMER_LEFT_49_SEQUENCE=GACATTTTCTTCCTTTCTGGAC
PRIMER_RIGHT_49_SEQUENCE=CATTGCTTTGACTGGAGAACC
PRIMER_LEFT_49=722,22
PRIMER_RIGHT_49=1412,21
PRIMER_LEFT_49_TM=56.943
PRIMER_RIGHT_49_TM=59.726
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=691
=
SEQUENCE_ID=MAGIC.149_1_11313052_11313123_oy-0_kn-0_2171_469424_TRUE
SEQUENCE_TARGET=950,171
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTGNNNNNCGATGATNTTTCNTTNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATNNTTNTNCTCTTCTNCAGTTACATTTGTTNCTGAGAGTCACAGTATTGTGGATACTTACANCTGCAAGGNTGATTNCTCACCTGCAAAANNNTNNTCTCTCAATATNGCCNCAAGANGCATANNNNNGATNGNNNNNAAANTATGNTTTACCTTNCGTAATGTGAAAGATACCCTTCTAGAACTTCTTCTGATCACTTTATCCTTCACCTTGTCAATCTATTAGAGNNCANTACANNGTTNAGCAATTNNNNNNNNNNNNNNNNNNNNTTATCCTGNNGAGAGCGAAGGAGAGATNGAAATGNAGANTACCAACCTTGTGATGTGGNATGTAGTGATTCCAAGCATATCGNGCTTCACCGGATAATAAGAGCATAGACCGAGGNGGAAGATATAAGGCCTTTTTNATGCAAGAGNAGTCACCCGACTTCNCAGCATCGGTAGTTGAGGCTTTCCATGTGGAAACAGANTATNTTCTGAATTCCATAATACAAGGACCAGCTAAAGAAAGGCTGAATATGCAATCTTCAAATGCTGAATGTGTATCNATNTGAGGCGATAANCCCACACCNGANGGGTACTCATTTACCTANNNNNNACAAGAATTCCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTNTATANAGAGTCTNATGATATACCGTNAANTGGTCCAAATNCAAGCNNNNNNNNNNNNNNNNNNNGNTCGGGAACAANNANATTCTTTCAAGTATAGGAGAAACAAANGANG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5818408_5818537_hi-0_oy-0_30831_57081_TRUE
SEQUENCE_TARGET=950,229
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_13491323_13491349_hi-0_wil-2_468_760452_TRUE
SEQUENCE_TARGET=950,126
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGGGTGAGCTTGNTNNNANNAATNNNAGGTGGANGNAGGAGGTGGCTGAGGGNNNGGAGGAGGTTTGGGGAGATGGTCCANCCATAGCGGCGGAGGCGGTGGAGGAGNAGATTNGAGANNNNNNNNNNNNNNNNNNNAGANGATNGTTGCTNCTNGANAGCNTTTTTTTCTTNGCTCAAAGTTATTGTNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAACACNATGTNANTNAGCAGNTNTTGAGCCAANTNGNTTCCTTTNNTTGGTATGGAGATGTGAAAANACANTCNAANTTCTCTCANAACCANTCGNANTANNCGTGAGACNAAGAATATTGCAAAGTNTNTANGTGTTGGTGTTGTGNCTCCATACGCAGANCACCANGTAGCTNGTGATTNCNTTTTTNNNNNNNNNNNNNNNCANTNGAATCCCAAAGAGTCCANAAGCACTNTTAAACTNNTNTANTTCGTCGCTTAACCNATNTTGAGTTGCANCANNAANAACCAACCTNTCAAATGTNTNNATAGNCCTCTNNCAACCTCTTCACAATGCACTNNNGACTNTGGATTATANTNAAATTNNNNATTCAAGANGTAGCCANNANGCAAAGGCTNATNGAGTTGANAATCTCANNGCNTATCGATAATTTCAANTGNATCTTTGTATTTNTCTTTTTTACCATNGAAGGTTTTANNAATGGCCNCTTTAGCCTNATCCATTNCTTCATAGATGTATCNNNTAGCTGNNTTNTTNTCTCCGTCAACCATNCTTGTGCCANAAANNNTCTTGCATAATGATCNTCTTCANAGTCCTTGCTCNTATTTCCTTTNNCNATTTGGAATCTNCCCATTNTTGANAAGTCNCNNAATTCCTCAAGTTTTTTCTNTGNNTATNATACTGNCCAANTGTNTNAAAGACGTAGCAAACCNTGTTACANCCNNTCTTTGCNGATTTCCATCGCTTNTGANNTTTNCTCATCNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAANCATTCNTGANGATGCATTGTNTGATAACAGTCTTNANTTCNNNAATCTTNCCTATATCCTCAAGCATGAGNTNTATGCAATGAGCANCANAAGGCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATAAANNAAACACAAAACGAGTTAGAAGNAGCNATGAAAAAACCAAACTCNTAAAGCTAAATNTAAANGATTNNAAANNCACTAGCTTTNACATAGTTTGATGNNTTGTCGNTTACCACTTNGACNANNNNAGCTTCNCCAACTTCATCCACCATANGATCNANNTGATCGTACAANATGTTAGCATCTTTCANAANTGTTGAAANATNAATTNATTTGATNNAAANNGATCNCTTTGGNNAGTTAACAAGAAAGTTGNNAATGTNTTTTGNACAANNNAATNTNGCCANCTNTNANANATTATTNAACAACCTTTANNTNCCCATTCTGACTTGTGCTCCACCAACTTTTTCTCTGNTTCANTGACTTNNTTCNTCAATAAAGGTACTCTTAGTTCATNNANGATTGGTGGCTTAANGCCTCCTTAAAACTATCATAGTTANTTGCATTAANNNNNNNNNNNNNNNNNNNNAACCACNTCGNAATAGCACTACATGCTNTATNCCTAAGTTNNTTNTCANACACTCNANNANTATTTATTNTNTTNTCTTTTCTACCTTTCAATACATNAGGANGNGTTGGA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_13105753_13105755_can-0_po-0_1999_19201_TRUE
SEQUENCE_TARGET=950,102
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_17416620_17417240_hi-0_ler-0_366244_110721_TRUE
SEQUENCE_TARGET=950,720
SEQUENCE_TEMPLATE=AAGTGATGGAAAAAGATTAAAATAAAATANAAATATGGATATACCCTTAAGATTCCGGANACTATGCTTCTCCGATCTCGTCTATAAATTTCAGCCGTCTCAGCCAACACAATCACAACNACCACCTCNCTNNCTCTCTCTCTCTNNATCTTTCTGCNCCCACCATTAGCGCACAACGGTGAGATTCGTTAGATGTTTATTATNTATGCATCCAAATTCACATATAGATANATAGNNNNNANATAGTTGTTTATATCAANANAAANNTTTGTTTCTAAATTNTNATCNCTTAAATTATTTGATTTATGTATAGNTTTAATTATAANTGTTATTCTGTTNGTTGTATTNGTACTAAACTNAAATAATNTANTAGNATCTTTCCAATNTTATTATAAAATTATNGTTAAGATATTTANTTTTANTTCATGNAGCAGGANAGACAAGAGAGGCTTAGATGGCTGAACAACAACTAGGNGTGCTAAAGGCACTCGATGTTGCGAAGACGCAACTTTATCATTTCACGGCGATTGTCATNGCCGGTATGGGTTTCTTTACNGATGCNTACGATCTTTTTTGCGTGTCCTTGGTAACAAAGCTCCTTGGCCGCATCTACTANTTCNATCCGGNGTCAGCGAAGCCTGGCTCACTTCCCCCTCATGTTGCGGCCGCNGTCAANGGTGTGGCCCTTTGTGGAACCCTTTCTGGTCAACTCTTNTTCGGTTGGCTCGGTGACAAACTCGGACGGAAAAAAGTGTACGGTCTNACTTTGNTNATGATGATCTTATGCTCTGTCGCTTCTGGCCTCTCNTTTGGCNACGAAGCCAAGGGTGTCATGACCACCCTTTGCTTCTTCAGGTANANTTTTCATCCAATTACAATANTATATANACANATGATTAACCAATAATCTNATAACGAATGCAANTTTTAAAAGTTAGTCACGCTTCGAACTGANNTAGGTNNTTTTCNTNNNNNNNNNNNTNTNATATATNAACNATTANNTAGGTTTTGGNTGGGATTTGGTATTGGAGGTGACTACCCACTTTCTGCCACCATCATGTCTGAATACGCAAACAAGAAGACCCGTGGGGCTTTCATCGCAGCTGTCTTCGCCATGCAAGGTGTCGGTATCTTGGCTGGAGGTTTCGTGGCACTCGCAGTATCTTCTATATTCGACAAAAAGTTCCCAGCTCCAACATATGCAGTAAACAGGGCNCTCTCAACGCCTCCTCAAGTTGATTACATTTGGCGAATCATCGTCATGTTTGGTGCTTTACCCGCAGCTTTGACTTACTACTGGCGTATGAAGATGCCTGAAACTGCCCGTTACACCGCTTTGGTTGCCAAGAACATCAAACAAGCCACAGCCGACATGTCCAAGGTCTTACAAACAGATNTCGAGCTTGAGGAAAGGGTGGAGGATGACGTCAAAGACCCCAGACAAAACTATGGCTTGTTCTCCAAGGAATTCCTTAGACGNCATGGACTTCATCTCCTTGGAACTACCTCCACTTGGTTTTTGCTTGACATTGCCTTCTACAGCCAAAACTTGTTCCAGAAGGATATTTTCTCGGCCATCGGATGGATCCCAAAGGCAGCCACCATGAACGCCACCCATGANGTTTTCAGGATTGCTAGGGCTCAGACTCTTATCGCCCTTTGCAGTACAGTCCCAGGCTACTGGTTCACAGTTGCGTTTATTGATANCATTGGAAGNTTTANGATCCAACTAAATGGATTTTTCATGATGACCGTGTTTATGTTTGCCATTGCNTTCCCTTACAACCACTGGATCNAACCAGANAACCGNATNGGATTNGTGNTNATGTACTCTCTNACTTTCTTCTTNGCCAATTTTGGTCCAAATGCAACCACTTTTATTGTCCCTGCTGAGATNTTNCCGGCCAGGCTAAGGTCNACATGTCATGGAATATCAGCNGCNGCTGGTAAGGCTGGAGCCATCATTGGAGCCTTCGGGTTCNTATATGCNGCTCAANNTCAAGACAAGGCTAAGGTGGATGCAGGATACCCACCAGGTATCGGAGTTAAGAACTCATTGATCGTNCTTGGTGTTCTTAACTTCATCGGTATGCTCTTCACCTTCCTTGTCCCAGAGCCNAAGGGCAAGTCCCTCGAAGAACTNTCTGGTGAAGCTGAAGTTAGCCATGACGAGAAATAATTTACTTTGTGATCAAATGTGGAGTTGTTTGTGGTTTGTTTGATTTTTTTGTTTCNTTNCANNNNNTTTTTTTCCTTTCCTTGATGCAGTAANAAATGTTTGATTGTGTGGNATACTTCANTCTTACTGCCGTGAGTGCAAATCTTGTAANCCACNTTTTTGTGTTCTAATTAACNTTGTCAAATNAAGTTTNTTAAACTTTGATTCTAACTTTNCAAACACTAACGAACTCTCTGTTGTGCGTTNNTGTTTATCAANTTGAAGCATGAAAATTTGATTGTTCATATGTTNNCTATNTACTGAGAAACACCANAGAATAGTTTTTATGGATTTNGATTATCTNATCAACACATTAGTTGGTTATGGTGTTCAATGTTCAGTGTCTTGTCATCNCTAACGTTATCTCTTTNCCTTATATCGACACATTCAAGTGGGCGTAGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.666778
PRIMER_LEFT_0_PENALTY=0.111077
PRIMER_RIGHT_0_PENALTY=0.555701
PRIMER_LEFT_0_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_0_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_0=831,20
PRIMER_RIGHT_0=1767,20
PRIMER_LEFT_0_TM=60.111
PRIMER_RIGHT_0_TM=59.444
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=937
PRIMER_PAIR_1_PENALTY=0.666778
PRIMER_LEFT_1_PENALTY=0.111077
PRIMER_RIGHT_1_PENALTY=0.555701
PRIMER_LEFT_1_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_1_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_1=831,20
PRIMER_RIGHT_1=1768,20
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=59.444
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=938
PRIMER_PAIR_2_PENALTY=0.788001
PRIMER_LEFT_2_PENALTY=0.232299
PRIMER_RIGHT_2_PENALTY=0.555701
PRIMER_LEFT_2_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_2_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_2=834,20
PRIMER_RIGHT_2=1767,20
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=59.444
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=934
PRIMER_PAIR_3_PENALTY=0.788001
PRIMER_LEFT_3_PENALTY=0.232299
PRIMER_RIGHT_3_PENALTY=0.555701
PRIMER_LEFT_3_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_3_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_3=834,20
PRIMER_RIGHT_3=1768,20
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=59.444
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=935
PRIMER_PAIR_4_PENALTY=0.788001
PRIMER_LEFT_4_PENALTY=0.232299
PRIMER_RIGHT_4_PENALTY=0.555701
PRIMER_LEFT_4_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_4_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_4=833,20
PRIMER_RIGHT_4=1767,20
PRIMER_LEFT_4_TM=60.232
PRIMER_RIGHT_4_TM=59.444
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=935
PRIMER_PAIR_5_PENALTY=0.788001
PRIMER_LEFT_5_PENALTY=0.232299
PRIMER_RIGHT_5_PENALTY=0.555701
PRIMER_LEFT_5_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_5_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_5=833,20
PRIMER_RIGHT_5=1768,20
PRIMER_LEFT_5_TM=60.232
PRIMER_RIGHT_5_TM=59.444
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=936
PRIMER_PAIR_6_PENALTY=1.227298
PRIMER_LEFT_6_PENALTY=0.671597
PRIMER_RIGHT_6_PENALTY=0.555701
PRIMER_LEFT_6_SEQUENCE=CACCCTTTGCTTCTTCAGGT
PRIMER_RIGHT_6_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_6=837,20
PRIMER_RIGHT_6=1767,20
PRIMER_LEFT_6_TM=59.328
PRIMER_RIGHT_6_TM=59.444
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=931
PRIMER_PAIR_7_PENALTY=1.227298
PRIMER_LEFT_7_PENALTY=0.671597
PRIMER_RIGHT_7_PENALTY=0.555701
PRIMER_LEFT_7_SEQUENCE=CACCCTTTGCTTCTTCAGGT
PRIMER_RIGHT_7_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_7=837,20
PRIMER_RIGHT_7=1768,20
PRIMER_LEFT_7_TM=59.328
PRIMER_RIGHT_7_TM=59.444
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=932
PRIMER_PAIR_8_PENALTY=1.366938
PRIMER_LEFT_8_PENALTY=0.111077
PRIMER_RIGHT_8_PENALTY=1.255861
PRIMER_LEFT_8_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_8_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_8=831,20
PRIMER_RIGHT_8=1769,21
PRIMER_LEFT_8_TM=60.111
PRIMER_RIGHT_8_TM=59.744
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=939
PRIMER_PAIR_9_PENALTY=1.381974
PRIMER_LEFT_9_PENALTY=0.826272
PRIMER_RIGHT_9_PENALTY=0.555701
PRIMER_LEFT_9_SEQUENCE=TTATGCTCTGTCGCTTCTGG
PRIMER_RIGHT_9_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_9=781,20
PRIMER_RIGHT_9=1767,20
PRIMER_LEFT_9_TM=59.174
PRIMER_RIGHT_9_TM=59.444
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=987
PRIMER_PAIR_10_PENALTY=1.381974
PRIMER_LEFT_10_PENALTY=0.826272
PRIMER_RIGHT_10_PENALTY=0.555701
PRIMER_LEFT_10_SEQUENCE=TTATGCTCTGTCGCTTCTGG
PRIMER_RIGHT_10_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_10=781,20
PRIMER_RIGHT_10=1768,20
PRIMER_LEFT_10_TM=59.174
PRIMER_RIGHT_10_TM=59.444
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=988
PRIMER_PAIR_11_PENALTY=1.488161
PRIMER_LEFT_11_PENALTY=0.232299
PRIMER_RIGHT_11_PENALTY=1.255861
PRIMER_LEFT_11_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_11_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_11=834,20
PRIMER_RIGHT_11=1769,21
PRIMER_LEFT_11_TM=60.232
PRIMER_RIGHT_11_TM=59.744
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=936
PRIMER_PAIR_12_PENALTY=1.488161
PRIMER_LEFT_12_PENALTY=0.232299
PRIMER_RIGHT_12_PENALTY=1.255861
PRIMER_LEFT_12_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_12_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_12=833,20
PRIMER_RIGHT_12=1769,21
PRIMER_LEFT_12_TM=60.232
PRIMER_RIGHT_12_TM=59.744
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=937
PRIMER_PAIR_13_PENALTY=1.601668
PRIMER_LEFT_13_PENALTY=1.045967
PRIMER_RIGHT_13_PENALTY=0.555701
PRIMER_LEFT_13_SEQUENCE=TCATGACCACCCTTTGCTTC
PRIMER_RIGHT_13_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_13=830,20
PRIMER_RIGHT_13=1767,20
PRIMER_LEFT_13_TM=61.046
PRIMER_RIGHT_13_TM=59.444
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=938
PRIMER_PAIR_14_PENALTY=1.601668
PRIMER_LEFT_14_PENALTY=1.045967
PRIMER_RIGHT_14_PENALTY=0.555701
PRIMER_LEFT_14_SEQUENCE=TCATGACCACCCTTTGCTTC
PRIMER_RIGHT_14_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_14=830,20
PRIMER_RIGHT_14=1768,20
PRIMER_LEFT_14_TM=61.046
PRIMER_RIGHT_14_TM=59.444
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=939
PRIMER_PAIR_15_PENALTY=1.697972
PRIMER_LEFT_15_PENALTY=0.111077
PRIMER_RIGHT_15_PENALTY=1.586895
PRIMER_LEFT_15_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_15_SEQUENCE=AATGGCAAACATAAACACGG
PRIMER_LEFT_15=831,20
PRIMER_RIGHT_15=1770,20
PRIMER_LEFT_15_TM=60.111
PRIMER_RIGHT_15_TM=58.413
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=940
PRIMER_PAIR_16_PENALTY=1.716792
PRIMER_LEFT_16_PENALTY=1.161090
PRIMER_RIGHT_16_PENALTY=0.555701
PRIMER_LEFT_16_SEQUENCE=CTTATGCTCTGTCGCTTCTGG
PRIMER_RIGHT_16_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_16=780,21
PRIMER_RIGHT_16=1767,20
PRIMER_LEFT_16_TM=60.161
PRIMER_RIGHT_16_TM=59.444
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=988
PRIMER_PAIR_17_PENALTY=1.716792
PRIMER_LEFT_17_PENALTY=1.161090
PRIMER_RIGHT_17_PENALTY=0.555701
PRIMER_LEFT_17_SEQUENCE=CTTATGCTCTGTCGCTTCTGG
PRIMER_RIGHT_17_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_17=780,21
PRIMER_RIGHT_17=1768,20
PRIMER_LEFT_17_TM=60.161
PRIMER_RIGHT_17_TM=59.444
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=989
PRIMER_PAIR_18_PENALTY=1.743316
PRIMER_LEFT_18_PENALTY=0.088413
PRIMER_RIGHT_18_PENALTY=1.654904
PRIMER_LEFT_18_SEQUENCE=GACAAACTCGGACGGAAAAA
PRIMER_RIGHT_18_SEQUENCE=TGTGAACCAGTAGCCTGGGA
PRIMER_LEFT_18=730,20
PRIMER_RIGHT_18=1689,20
PRIMER_LEFT_18_TM=60.088
PRIMER_RIGHT_18_TM=61.655
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=960
PRIMER_PAIR_19_PENALTY=1.765981
PRIMER_LEFT_19_PENALTY=0.111077
PRIMER_RIGHT_19_PENALTY=1.654904
PRIMER_LEFT_19_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_19_SEQUENCE=TGTGAACCAGTAGCCTGGGA
PRIMER_LEFT_19=831,20
PRIMER_RIGHT_19=1689,20
PRIMER_LEFT_19_TM=60.111
PRIMER_RIGHT_19_TM=61.655
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=859
PRIMER_PAIR_20_PENALTY=1.819194
PRIMER_LEFT_20_PENALTY=0.232299
PRIMER_RIGHT_20_PENALTY=1.586895
PRIMER_LEFT_20_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_20_SEQUENCE=AATGGCAAACATAAACACGG
PRIMER_LEFT_20=834,20
PRIMER_RIGHT_20=1770,20
PRIMER_LEFT_20_TM=60.232
PRIMER_RIGHT_20_TM=58.413
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=9.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=937
PRIMER_PAIR_21_PENALTY=1.819194
PRIMER_LEFT_21_PENALTY=0.232299
PRIMER_RIGHT_21_PENALTY=1.586895
PRIMER_LEFT_21_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_21_SEQUENCE=AATGGCAAACATAAACACGG
PRIMER_LEFT_21=833,20
PRIMER_RIGHT_21=1770,20
PRIMER_LEFT_21_TM=60.232
PRIMER_RIGHT_21_TM=58.413
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=938
PRIMER_PAIR_22_PENALTY=1.887203
PRIMER_LEFT_22_PENALTY=0.232299
PRIMER_RIGHT_22_PENALTY=1.654904
PRIMER_LEFT_22_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_22_SEQUENCE=TGTGAACCAGTAGCCTGGGA
PRIMER_LEFT_22=834,20
PRIMER_RIGHT_22=1689,20
PRIMER_LEFT_22_TM=60.232
PRIMER_RIGHT_22_TM=61.655
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=856
PRIMER_PAIR_23_PENALTY=1.887203
PRIMER_LEFT_23_PENALTY=0.232299
PRIMER_RIGHT_23_PENALTY=1.654904
PRIMER_LEFT_23_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_23_SEQUENCE=TGTGAACCAGTAGCCTGGGA
PRIMER_LEFT_23=833,20
PRIMER_RIGHT_23=1689,20
PRIMER_LEFT_23_TM=60.232
PRIMER_RIGHT_23_TM=61.655
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=857
PRIMER_PAIR_24_PENALTY=1.909854
PRIMER_LEFT_24_PENALTY=1.354152
PRIMER_RIGHT_24_PENALTY=0.555701
PRIMER_LEFT_24_SEQUENCE=ATGACCACCCTTTGCTTCTT
PRIMER_RIGHT_24_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_24=832,20
PRIMER_RIGHT_24=1768,20
PRIMER_LEFT_24_TM=58.646
PRIMER_RIGHT_24_TM=59.444
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=937
PRIMER_PAIR_25_PENALTY=1.927458
PRIMER_LEFT_25_PENALTY=0.671597
PRIMER_RIGHT_25_PENALTY=1.255861
PRIMER_LEFT_25_SEQUENCE=CACCCTTTGCTTCTTCAGGT
PRIMER_RIGHT_25_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_25=837,20
PRIMER_RIGHT_25=1769,21
PRIMER_LEFT_25_TM=59.328
PRIMER_RIGHT_25_TM=59.744
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=933
PRIMER_PAIR_26_PENALTY=2.046015
PRIMER_LEFT_26_PENALTY=1.490313
PRIMER_RIGHT_26_PENALTY=0.555701
PRIMER_LEFT_26_SEQUENCE=ATGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_26_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_26=832,21
PRIMER_RIGHT_26=1767,20
PRIMER_LEFT_26_TM=60.490
PRIMER_RIGHT_26_TM=59.444
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=936
PRIMER_PAIR_27_PENALTY=2.046015
PRIMER_LEFT_27_PENALTY=1.490313
PRIMER_RIGHT_27_PENALTY=0.555701
PRIMER_LEFT_27_SEQUENCE=ATGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_27_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_27=832,21
PRIMER_RIGHT_27=1768,20
PRIMER_LEFT_27_TM=60.490
PRIMER_RIGHT_27_TM=59.444
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=937
PRIMER_PAIR_28_PENALTY=2.082134
PRIMER_LEFT_28_PENALTY=0.826272
PRIMER_RIGHT_28_PENALTY=1.255861
PRIMER_LEFT_28_SEQUENCE=TTATGCTCTGTCGCTTCTGG
PRIMER_RIGHT_28_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_28=781,20
PRIMER_RIGHT_28=1769,21
PRIMER_LEFT_28_TM=59.174
PRIMER_RIGHT_28_TM=59.744
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=989
PRIMER_PAIR_29_PENALTY=2.127591
PRIMER_LEFT_29_PENALTY=0.088413
PRIMER_RIGHT_29_PENALTY=2.039178
PRIMER_LEFT_29_SEQUENCE=GACAAACTCGGACGGAAAAA
PRIMER_RIGHT_29_SEQUENCE=TCAATAAACGCAACTGTGAACC
PRIMER_LEFT_29=730,20
PRIMER_RIGHT_29=1703,22
PRIMER_LEFT_29_TM=60.088
PRIMER_RIGHT_29_TM=60.039
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=974
PRIMER_PAIR_30_PENALTY=2.150255
PRIMER_LEFT_30_PENALTY=0.111077
PRIMER_RIGHT_30_PENALTY=2.039178
PRIMER_LEFT_30_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_30_SEQUENCE=TCAATAAACGCAACTGTGAACC
PRIMER_LEFT_30=831,20
PRIMER_RIGHT_30=1703,22
PRIMER_LEFT_30_TM=60.111
PRIMER_RIGHT_30_TM=60.039
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=873
PRIMER_PAIR_31_PENALTY=2.213440
PRIMER_LEFT_31_PENALTY=0.111077
PRIMER_RIGHT_31_PENALTY=2.102363
PRIMER_LEFT_31_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_31_SEQUENCE=GATCCAGTGGTTGTAAGGGAA
PRIMER_LEFT_31=831,20
PRIMER_RIGHT_31=1794,21
PRIMER_LEFT_31_TM=60.111
PRIMER_RIGHT_31_TM=58.898
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=964
PRIMER_PAIR_32_PENALTY=2.254210
PRIMER_LEFT_32_PENALTY=0.111077
PRIMER_RIGHT_32_PENALTY=2.143133
PRIMER_LEFT_32_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_32_SEQUENCE=CAATGGCAAACATAAACACGG
PRIMER_LEFT_32=831,20
PRIMER_RIGHT_32=1771,21
PRIMER_LEFT_32_TM=60.111
PRIMER_RIGHT_32_TM=61.143
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=941
PRIMER_PAIR_33_PENALTY=2.258492
PRIMER_LEFT_33_PENALTY=0.671597
PRIMER_RIGHT_33_PENALTY=1.586895
PRIMER_LEFT_33_SEQUENCE=CACCCTTTGCTTCTTCAGGT
PRIMER_RIGHT_33_SEQUENCE=AATGGCAAACATAAACACGG
PRIMER_LEFT_33=837,20
PRIMER_RIGHT_33=1770,20
PRIMER_LEFT_33_TM=59.328
PRIMER_RIGHT_33_TM=58.413
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=9.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=934
PRIMER_PAIR_34_PENALTY=2.271478
PRIMER_LEFT_34_PENALTY=0.232299
PRIMER_RIGHT_34_PENALTY=2.039178
PRIMER_LEFT_34_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_34_SEQUENCE=TCAATAAACGCAACTGTGAACC
PRIMER_LEFT_34=834,20
PRIMER_RIGHT_34=1703,22
PRIMER_LEFT_34_TM=60.232
PRIMER_RIGHT_34_TM=60.039
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=870
PRIMER_PAIR_35_PENALTY=2.271478
PRIMER_LEFT_35_PENALTY=0.232299
PRIMER_RIGHT_35_PENALTY=2.039178
PRIMER_LEFT_35_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_35_SEQUENCE=TCAATAAACGCAACTGTGAACC
PRIMER_LEFT_35=833,20
PRIMER_RIGHT_35=1703,22
PRIMER_LEFT_35_TM=60.232
PRIMER_RIGHT_35_TM=60.039
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=871
PRIMER_PAIR_36_PENALTY=2.301828
PRIMER_LEFT_36_PENALTY=1.045967
PRIMER_RIGHT_36_PENALTY=1.255861
PRIMER_LEFT_36_SEQUENCE=TCATGACCACCCTTTGCTTC
PRIMER_RIGHT_36_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_36=830,20
PRIMER_RIGHT_36=1769,21
PRIMER_LEFT_36_TM=61.046
PRIMER_RIGHT_36_TM=59.744
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=9.6000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=940
PRIMER_PAIR_37_PENALTY=2.326501
PRIMER_LEFT_37_PENALTY=0.671597
PRIMER_RIGHT_37_PENALTY=1.654904
PRIMER_LEFT_37_SEQUENCE=CACCCTTTGCTTCTTCAGGT
PRIMER_RIGHT_37_SEQUENCE=TGTGAACCAGTAGCCTGGGA
PRIMER_LEFT_37=837,20
PRIMER_RIGHT_37=1689,20
PRIMER_LEFT_37_TM=59.328
PRIMER_RIGHT_37_TM=61.655
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=853
PRIMER_PAIR_38_PENALTY=2.334663
PRIMER_LEFT_38_PENALTY=0.232299
PRIMER_RIGHT_38_PENALTY=2.102363
PRIMER_LEFT_38_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_38_SEQUENCE=GATCCAGTGGTTGTAAGGGAA
PRIMER_LEFT_38=834,20
PRIMER_RIGHT_38=1794,21
PRIMER_LEFT_38_TM=60.232
PRIMER_RIGHT_38_TM=58.898
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=961
PRIMER_PAIR_39_PENALTY=2.334663
PRIMER_LEFT_39_PENALTY=0.232299
PRIMER_RIGHT_39_PENALTY=2.102363
PRIMER_LEFT_39_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_39_SEQUENCE=GATCCAGTGGTTGTAAGGGAA
PRIMER_LEFT_39=833,20
PRIMER_RIGHT_39=1794,21
PRIMER_LEFT_39_TM=60.232
PRIMER_RIGHT_39_TM=58.898
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=962
PRIMER_PAIR_40_PENALTY=2.354767
PRIMER_LEFT_40_PENALTY=0.699863
PRIMER_RIGHT_40_PENALTY=1.654904
PRIMER_LEFT_40_SEQUENCE=GTTGGCTCGGTGACAAACTC
PRIMER_RIGHT_40_SEQUENCE=TGTGAACCAGTAGCCTGGGA
PRIMER_LEFT_40=719,20
PRIMER_RIGHT_40=1689,20
PRIMER_LEFT_40_TM=60.700
PRIMER_RIGHT_40_TM=61.655
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=971
PRIMER_PAIR_41_PENALTY=2.375432
PRIMER_LEFT_41_PENALTY=0.232299
PRIMER_RIGHT_41_PENALTY=2.143133
PRIMER_LEFT_41_SEQUENCE=GACCACCCTTTGCTTCTTCA
PRIMER_RIGHT_41_SEQUENCE=CAATGGCAAACATAAACACGG
PRIMER_LEFT_41=834,20
PRIMER_RIGHT_41=1771,21
PRIMER_LEFT_41_TM=60.232
PRIMER_RIGHT_41_TM=61.143
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=9.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=938
PRIMER_PAIR_42_PENALTY=2.375432
PRIMER_LEFT_42_PENALTY=0.232299
PRIMER_RIGHT_42_PENALTY=2.143133
PRIMER_LEFT_42_SEQUENCE=TGACCACCCTTTGCTTCTTC
PRIMER_RIGHT_42_SEQUENCE=CAATGGCAAACATAAACACGG
PRIMER_LEFT_42=833,20
PRIMER_RIGHT_42=1771,21
PRIMER_LEFT_42_TM=60.232
PRIMER_RIGHT_42_TM=61.143
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=9.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=939
PRIMER_PAIR_43_PENALTY=2.413167
PRIMER_LEFT_43_PENALTY=0.826272
PRIMER_RIGHT_43_PENALTY=1.586895
PRIMER_LEFT_43_SEQUENCE=TTATGCTCTGTCGCTTCTGG
PRIMER_RIGHT_43_SEQUENCE=AATGGCAAACATAAACACGG
PRIMER_LEFT_43=781,20
PRIMER_RIGHT_43=1770,20
PRIMER_LEFT_43_TM=59.174
PRIMER_RIGHT_43_TM=58.413
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=9.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=990
PRIMER_PAIR_44_PENALTY=2.416952
PRIMER_LEFT_44_PENALTY=1.161090
PRIMER_RIGHT_44_PENALTY=1.255861
PRIMER_LEFT_44_SEQUENCE=CTTATGCTCTGTCGCTTCTGG
PRIMER_RIGHT_44_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_44=780,21
PRIMER_RIGHT_44=1769,21
PRIMER_LEFT_44_TM=60.161
PRIMER_RIGHT_44_TM=59.744
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.6000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=990
PRIMER_PAIR_45_PENALTY=2.610014
PRIMER_LEFT_45_PENALTY=1.354152
PRIMER_RIGHT_45_PENALTY=1.255861
PRIMER_LEFT_45_SEQUENCE=ATGACCACCCTTTGCTTCTT
PRIMER_RIGHT_45_SEQUENCE=ATGGCAAACATAAACACGGTC
PRIMER_LEFT_45=832,20
PRIMER_RIGHT_45=1769,21
PRIMER_LEFT_45_TM=58.646
PRIMER_RIGHT_45_TM=59.744
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=9.6000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=938
PRIMER_PAIR_46_PENALTY=2.613499
PRIMER_LEFT_46_PENALTY=2.057797
PRIMER_RIGHT_46_PENALTY=0.555701
PRIMER_LEFT_46_SEQUENCE=CCACCCTTTGCTTCTTCAGG
PRIMER_RIGHT_46_SEQUENCE=GGCAAACATAAACACGGTCA
PRIMER_LEFT_46=836,20
PRIMER_RIGHT_46=1767,20
PRIMER_LEFT_46_TM=62.058
PRIMER_RIGHT_46_TM=59.444
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=932
PRIMER_PAIR_47_PENALTY=2.613499
PRIMER_LEFT_47_PENALTY=2.057797
PRIMER_RIGHT_47_PENALTY=0.555701
PRIMER_LEFT_47_SEQUENCE=CCACCCTTTGCTTCTTCAGG
PRIMER_RIGHT_47_SEQUENCE=TGGCAAACATAAACACGGTC
PRIMER_LEFT_47=836,20
PRIMER_RIGHT_47=1768,20
PRIMER_LEFT_47_TM=62.058
PRIMER_RIGHT_47_TM=59.444
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=9.6000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=933
PRIMER_PAIR_48_PENALTY=2.632862
PRIMER_LEFT_48_PENALTY=1.045967
PRIMER_RIGHT_48_PENALTY=1.586895
PRIMER_LEFT_48_SEQUENCE=TCATGACCACCCTTTGCTTC
PRIMER_RIGHT_48_SEQUENCE=AATGGCAAACATAAACACGG
PRIMER_LEFT_48=830,20
PRIMER_RIGHT_48=1770,20
PRIMER_LEFT_48_TM=61.046
PRIMER_RIGHT_48_TM=58.413
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=941
PRIMER_PAIR_49_PENALTY=2.689911
PRIMER_LEFT_49_PENALTY=0.111077
PRIMER_RIGHT_49_PENALTY=2.578834
PRIMER_LEFT_49_SEQUENCE=CATGACCACCCTTTGCTTCT
PRIMER_RIGHT_49_SEQUENCE=GATCCAGTGGTTGTAAGGGA
PRIMER_LEFT_49=831,20
PRIMER_RIGHT_49=1794,20
PRIMER_LEFT_49_TM=60.111
PRIMER_RIGHT_49_TM=57.421
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=964
=
SEQUENCE_ID=MAGIC.446_2_5741683_5742379_po-0_ct-1_80855_30182_TRUE
SEQUENCE_TARGET=950,796
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGGTGAGTACTCCTCTCGCNATCATCAGTCTTTACTCTGTTCCAAGTCTTCCTTCTTTGGCTCAAATGGGGTCCACGNTNATCAANCCAAANAGATNCGACTTTTTNNNCTGGGGTTTCGTCGAGAATTCGATTTTNGGGTTTCNTTCGAAACTTGGTCAAATGTCGAATACGCGTTACNAAGAACAAACCTTTNTCTTTATTTCNTTTGTCTTCGTCAACNGCTGAATCAACCAAANGGTCCCTGGAATNAATAAACCTCTAATAATAATGGCTTTGCTTTTACTCTGATGNCAAGTTCAAAAATGGAACAAAGATCACTCCTTTGCTTCCTTTATCTNCTNCTNCTATTCAATTTCACTCTCAGAGTCGCTGGAAACGCTGAAGGTTCTTCNTTTNTNTGCTACTCTCATGAAAAGTCCCTTCNTTTNTGGTNGTTGTTAGATTTNGAAAGTTCNTANCTTTNTTNNNNNNNNNNNNNNNNNNNNNTATGAATNNGTGTACAGGTGATGCTTTGACTCAGCTGAANAACAGTTTNTCATCAGGTGACCCNGCAAACAATGTACTCCAAAGCTGGGATGCTACTCTTGTTACTCCATGTACTTGGTTTCATGTTACTTGCAATCCTGAGAATAAAGTTACTCGTGTGTATGTTTCTCTNNNNNNNNNNNNNNNNNNNNNNNNNNTGCTNGTTTTNTCTGAATGAANGNTTCAANAATNCCTTTGNNAATNTACTGTTTGATGTTTGNTTAGTGACCTTGGGAATGCAAAACTATCTGGAAAGTTGGTNCCAGANCTTGGTCAGCTTTNAAACTTGCAGTANTTGTAAGCCATCTCTTCCTCAATGTCNATTAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNTNNGTNTNNNCTTTCNAAAGAGAATTGTGGATAAACAANTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATTTTAGATCTCTTTAGCTAGGCTTCTCGAGCCTTGTTACTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATTAGATAAGTANCTNGAGAGTTTGGATCNGGTATTTCTCTACAGGTGATCTCAACTCTNCATATTTGTGNTNCNGCTGCNAAATGTTTTNTTGAAANTATTGCTTACANATATAGTTTCCACNTTCATGTANNNNNNNNNTCTACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATACTTCATTANGTTTTCTGTANCNTNAANTTCTGGTAAAATANNNNTAGAGATTTTCCANTCNACAATNATTAANCAAANNNNNNNNNNNNNNNNGCTTAATGACAGGGAGCTTTANAGCAATAACATTACNGGGGAGATACCTGAGGAGCTTGGNGACTTGGTGGAACTAGTAAGCTTGGATCTTTACGCNAACANCATAAGCGGTCCCATCCCTTCNTCNCTTGGCAAACTAGGAAAACTNCGNTTCTTGTATGNANNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_8110620_8110686_ct-1_wu-0_1609_97290_TRUE
SEQUENCE_TARGET=950,166
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTCTATTGANAAGGCNAGTTGTTGTTGCTTGNATANCTTTCATTCNGGATACCANTNGNGCNATGCCNNTTNGGNNTNNNNNTCTCCCCAANNNNNNNNNNNNNNNNNAGTTGTTGTTGCTTGNATAGCTTTCATTCTGGATACCANTNGTGCNATGCCTGCCCTNNNATNCANANNNNNNNNNNNTTGTGAAAAAGACCATTTCTNCCNCAAACTNAACNACNCATTNCTTTATCTGANTAAGGAAAATGTTCATCCNATCATGATCATTTGACTGCGTAGAGGTCTCNGAATCATCGAGACAAAACCCGCTTNCATCATCTTNNGCCANCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTCTCCNANCTCGAGAGATATGNAACNTGAATCANAGTTGTAAAGANCNCAATTGTNAGGTGTTNAGACNTTTTTTGTTTTTNTNTAAATAGAANNNNNNNNNNNNNAAATTTNANAACGTTTAGGANTNGCAAAAATCTTGTGATACNNTGTACATANANTTTNATTTAATGTGATGANCTGTGATTTANNTTTTTNTTTTTATNTNAGTTGAAATTTANCTTTTGGAGAATATCNTAAATNCCATANTTCNANTAAATNATAGAAANATAAAGANTTTTTGTTGAAGATTNAAAGAAACTTTGACCAAATNTATNTAGAAGAGATNTTTNACCNTTTTAAACAATGAGATTATATCNTCCATAAACATNNNNNNNNNNNNNNNNAANNGGATTTCTTACCCAATNCTNCTTATTTATTTTCNAACTTAACATTTTAGGCCTTGANACAATATNAGAATCNGANATGACCAANACCAAACCANACTGNCCNGTTNACCANANNNNNNNNNNNNNNNNNNNNNNNNNNANGGTTGCTTACTCATATGCAAATCAACCCTAAGANNNNNNNNNNNNNAGGAGAAAANACAAATCAAAGATGGTCCTNGNCGACTCACCCGGACCACACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNAACNAAATTGAAGATTCATCAACAAACNACACATTTTAAGNTCGNTTGATCAANGAATAATCATAAANNAANGAGCNAGTCCACTAGAATGCCNTGATNACCCACNTNNATGAAGCCANTTTCACNTTCATCACCCCAAAGNCAACTTTCATCGNCCCATATTCGACATTNANTGATATNATNNNNNTTTATGAAGAATGGGCNAGTCATTNNNNNNNNNNACCGAACCATTCANCTAGTTNTTANGNGANCCTTCCCGAATCGTACNAAGTTNAGTTTCNNCAATCAAATGACANATTTCCTAGAACNNTATTGAAATTTCACCAACCAACAACCTATTTTATGAAGAATTGGTGAGGAANATTTGTGTGTTATCAAGGTATATCGAAGATGGTCGAGAGACACTCAATTCAGACTATACGAGCACCTACCTAGAGCTGTTTAAGGAAAGTTNTTTTTTTCTTTCCTTTGTATGCATCTCAAAGCCACATATGGAAACTAAGAGATCTTGTACACATTCTAGAAGCTTNTCCACACGTCCAGTGGTNCNCTTTCCCCACTTGATCATGCAAAGANCAAACTTGCCATATTTGGANCTNGAANGTTTAATTCACAAAAACTCTTTTCTTGTCACTGCTGCTGNGTTTTTAAGGCCCTCCNCATGTCCCACAATTTCCCAAGATANTACANAANT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_11096793_11096908_sf-2_hi-0_8778_2673683_TRUE
SEQUENCE_TARGET=950,215
SEQUENCE_TEMPLATE=AAAGGCTNAAGCCNAGTAAATANGTCGAACNGANTAAACCCGAGTTGGGGAGTTCGCGGATTCAAGTNCGCATTNCTTAGAGATAGGTNTGATCAACNTACGAATCTCGTGACTTGACNACATGGTTAAACAAAAATGCTATATAAAAGGTGGANTTTGCNAATCNATACTTGAATCNAGCGCAAAGAAANCCCTCTTNGGTTCATAGNCTTAAAAAATAATAATAAAGNNTTAAAAGCCTCCTCAGGCCAAGTATTTGTTTAGCCTACTTAGGCAGGTAAAATAGTAATAGTAAACTTTNAGTCTGTTNACAAAAAGGNACAATCCCCATTAAGTGGACANANCCACTACNGNTNCTCAGGCACCTCGAGTTCGGGTTGAGCNGNCTCANGATCAACTNAATCGGCGNCAGCTNTCTCNNTCTCACCCGNCTCGCCNNTAGTAGGCTGCTCGNTAGGAACCNTGGAATCTGAACCATCATCNTCCTCGATGACCACTGTNTCCGAGTTCNTGGATGGAGGAATNTCTGGCGGNGTGGNTACCGCCTCCGGAATTNTCNTNGGCACGATGGATGCTTCGAGCNCCACGGGATCGCTAGCACCTGAGNCAATTATCTCGGATTAGGAAAAATNCAAGAGTAATTNGCTGTGNATTGGTTTCAAAAGTTTAGGAATGTACNAGCCCAATCTAAANCTGGCATCNTTTAAGAACGGAGATCGAACTCAAGGGACGAAGANTTGTCTTTCCACGTCATAACCTCNTTTTCCCTGTCTAGCAACAGTCTCTCGTANTACTCAATCATGTCATTNAGTTCGCNGTTTGCCTGAACNAAGTNGTAACAAAAAAGAGTTAGCATCTAAGTGNAGGATTAAAGTTTAAANNNNNATTATGGGTGAAGNGAACTAACCNGAGATGAGTGATGGGNNTGGAGTAGGAATNGCNTGCGAATTGCAGGACGCCANCTATCAAANNNNGGCAGGATGGTGTCTTTTCAAGNTTTNAAGCACCGAAAGACGTTTCCCTCTGTTTTCATCGCTGTTAGAATAAAGAAAGGGTTGGACCAGGCAAGGAGCGTTNAGTCANTTAATCGAAATGATAAGGCCNGAAAAAAAAATNNAAGGTAATTTCTAACNCGTGAACGACTTGGTCTGAGGAACNANAACAGCCTNAGTTCNNGCATCTCGGACCTCAGCTTCTTCTCNNNGGGGGTTTGCACTCTGATCNNCCTTTTCACCTCTGGATCCAGACNANGGAGATACGGTGACTGGAGGANGAGGNCCCATAGAGCGGGGTGGTGGCGATGGCCGAGGAGAGNGATCAGTTCGGGCTCTCGTCNTATCTCTTTTGGGGGAGGAGCTACCNCGGGTTGATCTGTCTCTCTATTTCNGAATNAGGTCAGCAAGCGGCTCGCTGCTTAGTCTAGTTCGGGAGGCAGGCCGAGGTGATTNGNGGCGCGGTGATCGTTCTCTNCNGGATGACTGGCCGCTATGGCCTTCGCGGTGAGGAATGGGTCTGTAGGCACCAGCAGATNTTGCCCCTTCGGCGCTNCCATNGTCANCTAGCAAGGTTCTGCTATATTNTTTCTTGTCGACTGCGGATTGAAGGAATGATTTGTTCGTTTNGCAAGCCTCCACGATCGACTTGGATAGCNTCTNGCGTCGAGNTGCGATGACCCGAGCAGAAGTCCCTTGAGGTAAGGGAGGGGGCAATGGCAGTTGGAGAAGATGTCNAGGGGGGCTCATNCTTAGCTTGTGGTNNGCCTCTACGAATCNGTCGAGAGTNAAGGAGTTCCAGAGAGTTCNAGAGAGTTTCCCNTTCGGCNAATTCAANAGTCGGAAGGGTCGGTGCGATGAAAGCTGCNAATTGAAAATTAAGAGTTGGTNANATAAGGNAAGAAGTAAATAANGGTCATATGCATGACCTAACATCTANNGGGCTTAGTAGTCNACTTCGAGGAGAGCAAGTCCGAAAGGTTTCCAATCGAAGCNNGNCTCTTTTTGATTAAGNACCACNNGTGATGCCAGTTCTCGTCNTTATTAGGGAGATGCGAGATNAGGTTCCTGTTNGCNTTCNGATNGGCCGAGAAGTATCCTACCTTCTTTGAAGAACTCCTCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.205903
PRIMER_LEFT_0_PENALTY=0.195358
PRIMER_RIGHT_0_PENALTY=0.010545
PRIMER_LEFT_0_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_0_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_0=714,20
PRIMER_RIGHT_0=1524,20
PRIMER_LEFT_0_TM=60.195
PRIMER_RIGHT_0_TM=59.989
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=811
PRIMER_PAIR_1_PENALTY=0.207448
PRIMER_LEFT_1_PENALTY=0.196903
PRIMER_RIGHT_1_PENALTY=0.010545
PRIMER_LEFT_1_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_1_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_1=713,20
PRIMER_RIGHT_1=1524,20
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.989
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=812
PRIMER_PAIR_2_PENALTY=0.268309
PRIMER_LEFT_2_PENALTY=0.195358
PRIMER_RIGHT_2_PENALTY=0.072951
PRIMER_LEFT_2_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_2_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_2=714,20
PRIMER_RIGHT_2=1519,20
PRIMER_LEFT_2_TM=60.195
PRIMER_RIGHT_2_TM=60.073
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=806
PRIMER_PAIR_3_PENALTY=0.269854
PRIMER_LEFT_3_PENALTY=0.196903
PRIMER_RIGHT_3_PENALTY=0.072951
PRIMER_LEFT_3_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_3_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_3=713,20
PRIMER_RIGHT_3=1519,20
PRIMER_LEFT_3_TM=59.803
PRIMER_RIGHT_3_TM=60.073
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=807
PRIMER_PAIR_4_PENALTY=0.269854
PRIMER_LEFT_4_PENALTY=0.196903
PRIMER_RIGHT_4_PENALTY=0.072951
PRIMER_LEFT_4_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_4_SEQUENCE=TGCCTACAGACCCATTCCTC
PRIMER_LEFT_4=713,20
PRIMER_RIGHT_4=1520,20
PRIMER_LEFT_4_TM=59.803
PRIMER_RIGHT_4_TM=60.073
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=808
PRIMER_PAIR_5_PENALTY=0.640679
PRIMER_LEFT_5_PENALTY=0.195358
PRIMER_RIGHT_5_PENALTY=0.445321
PRIMER_LEFT_5_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_5_SEQUENCE=AGAAGCTGAGGTCCGAGATG
PRIMER_LEFT_5=714,20
PRIMER_RIGHT_5=1196,20
PRIMER_LEFT_5_TM=60.195
PRIMER_RIGHT_5_TM=59.555
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=483
PRIMER_PAIR_6_PENALTY=0.642224
PRIMER_LEFT_6_PENALTY=0.196903
PRIMER_RIGHT_6_PENALTY=0.445321
PRIMER_LEFT_6_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_6_SEQUENCE=AGAAGCTGAGGTCCGAGATG
PRIMER_LEFT_6=713,20
PRIMER_RIGHT_6=1196,20
PRIMER_LEFT_6_TM=59.803
PRIMER_RIGHT_6_TM=59.555
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=484
PRIMER_PAIR_7_PENALTY=0.665358
PRIMER_LEFT_7_PENALTY=0.195358
PRIMER_RIGHT_7_PENALTY=0.470001
PRIMER_LEFT_7_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_7_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_7=714,20
PRIMER_RIGHT_7=1270,20
PRIMER_LEFT_7_TM=60.195
PRIMER_RIGHT_7_TM=59.530
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=60.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.4000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=557
PRIMER_PAIR_8_PENALTY=0.666904
PRIMER_LEFT_8_PENALTY=0.196903
PRIMER_RIGHT_8_PENALTY=0.470001
PRIMER_LEFT_8_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_8_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_8=713,20
PRIMER_RIGHT_8=1270,20
PRIMER_LEFT_8_TM=59.803
PRIMER_RIGHT_8_TM=59.530
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=60.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=558
PRIMER_PAIR_9_PENALTY=0.726671
PRIMER_LEFT_9_PENALTY=0.716126
PRIMER_RIGHT_9_PENALTY=0.010545
PRIMER_LEFT_9_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_9_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_9=715,20
PRIMER_RIGHT_9=1524,20
PRIMER_LEFT_9_TM=59.284
PRIMER_RIGHT_9_TM=59.989
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.7000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=810
PRIMER_PAIR_10_PENALTY=0.764708
PRIMER_LEFT_10_PENALTY=0.195358
PRIMER_RIGHT_10_PENALTY=0.569350
PRIMER_LEFT_10_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_10_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_10=714,20
PRIMER_RIGHT_10=1359,20
PRIMER_LEFT_10_TM=60.195
PRIMER_RIGHT_10_TM=60.569
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=646
PRIMER_PAIR_11_PENALTY=0.766253
PRIMER_LEFT_11_PENALTY=0.196903
PRIMER_RIGHT_11_PENALTY=0.569350
PRIMER_LEFT_11_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_11_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_11=713,20
PRIMER_RIGHT_11=1359,20
PRIMER_LEFT_11_TM=59.803
PRIMER_RIGHT_11_TM=60.569
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=647
PRIMER_PAIR_12_PENALTY=0.789077
PRIMER_LEFT_12_PENALTY=0.716126
PRIMER_RIGHT_12_PENALTY=0.072951
PRIMER_LEFT_12_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_12_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_12=715,20
PRIMER_RIGHT_12=1519,20
PRIMER_LEFT_12_TM=59.284
PRIMER_RIGHT_12_TM=60.073
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.7000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=805
PRIMER_PAIR_13_PENALTY=0.789077
PRIMER_LEFT_13_PENALTY=0.716126
PRIMER_RIGHT_13_PENALTY=0.072951
PRIMER_LEFT_13_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_13_SEQUENCE=TGCCTACAGACCCATTCCTC
PRIMER_LEFT_13=715,20
PRIMER_RIGHT_13=1520,20
PRIMER_LEFT_13_TM=59.284
PRIMER_RIGHT_13_TM=60.073
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.7000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=806
PRIMER_PAIR_14_PENALTY=0.889206
PRIMER_LEFT_14_PENALTY=0.195358
PRIMER_RIGHT_14_PENALTY=0.693848
PRIMER_LEFT_14_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_14_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_14=714,20
PRIMER_RIGHT_14=1358,20
PRIMER_LEFT_14_TM=60.195
PRIMER_RIGHT_14_TM=60.694
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=645
PRIMER_PAIR_15_PENALTY=0.890751
PRIMER_LEFT_15_PENALTY=0.196903
PRIMER_RIGHT_15_PENALTY=0.693848
PRIMER_LEFT_15_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_15_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_15=713,20
PRIMER_RIGHT_15=1358,20
PRIMER_LEFT_15_TM=59.803
PRIMER_RIGHT_15_TM=60.694
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=646
PRIMER_PAIR_16_PENALTY=0.923246
PRIMER_LEFT_16_PENALTY=0.912701
PRIMER_RIGHT_16_PENALTY=0.010545
PRIMER_LEFT_16_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_16_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_16=716,20
PRIMER_RIGHT_16=1524,20
PRIMER_LEFT_16_TM=60.913
PRIMER_RIGHT_16_TM=59.989
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=809
PRIMER_PAIR_17_PENALTY=0.971076
PRIMER_LEFT_17_PENALTY=0.195358
PRIMER_RIGHT_17_PENALTY=0.775718
PRIMER_LEFT_17_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_17_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_17=714,20
PRIMER_RIGHT_17=1599,20
PRIMER_LEFT_17_TM=60.195
PRIMER_RIGHT_17_TM=60.776
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=886
PRIMER_PAIR_18_PENALTY=0.972621
PRIMER_LEFT_18_PENALTY=0.196903
PRIMER_RIGHT_18_PENALTY=0.775718
PRIMER_LEFT_18_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_18_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_18=713,20
PRIMER_RIGHT_18=1599,20
PRIMER_LEFT_18_TM=59.803
PRIMER_RIGHT_18_TM=60.776
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=887
PRIMER_PAIR_19_PENALTY=0.985652
PRIMER_LEFT_19_PENALTY=0.912701
PRIMER_RIGHT_19_PENALTY=0.072951
PRIMER_LEFT_19_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_19_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_19=716,20
PRIMER_RIGHT_19=1519,20
PRIMER_LEFT_19_TM=60.913
PRIMER_RIGHT_19_TM=60.073
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=804
PRIMER_PAIR_20_PENALTY=0.985652
PRIMER_LEFT_20_PENALTY=0.912701
PRIMER_RIGHT_20_PENALTY=0.072951
PRIMER_LEFT_20_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_20_SEQUENCE=TGCCTACAGACCCATTCCTC
PRIMER_LEFT_20=716,20
PRIMER_RIGHT_20=1520,20
PRIMER_LEFT_20_TM=60.913
PRIMER_RIGHT_20_TM=60.073
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=805
PRIMER_PAIR_21_PENALTY=1.119143
PRIMER_LEFT_21_PENALTY=0.195358
PRIMER_RIGHT_21_PENALTY=0.923785
PRIMER_LEFT_21_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_21_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_21=714,20
PRIMER_RIGHT_21=1523,20
PRIMER_LEFT_21_TM=60.195
PRIMER_RIGHT_21_TM=60.924
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=810
PRIMER_PAIR_22_PENALTY=1.120688
PRIMER_LEFT_22_PENALTY=0.196903
PRIMER_RIGHT_22_PENALTY=0.923785
PRIMER_LEFT_22_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_22_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_22=713,20
PRIMER_RIGHT_22=1523,20
PRIMER_LEFT_22_TM=59.803
PRIMER_RIGHT_22_TM=60.924
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=811
PRIMER_PAIR_23_PENALTY=1.161447
PRIMER_LEFT_23_PENALTY=0.716126
PRIMER_RIGHT_23_PENALTY=0.445321
PRIMER_LEFT_23_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_23_SEQUENCE=AGAAGCTGAGGTCCGAGATG
PRIMER_LEFT_23=715,20
PRIMER_RIGHT_23=1196,20
PRIMER_LEFT_23_TM=59.284
PRIMER_RIGHT_23_TM=59.555
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.7000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=482
PRIMER_PAIR_24_PENALTY=1.177619
PRIMER_LEFT_24_PENALTY=0.195358
PRIMER_RIGHT_24_PENALTY=0.982262
PRIMER_LEFT_24_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_24_SEQUENCE=GTGCCTACAGACCCATTCCT
PRIMER_LEFT_24=714,20
PRIMER_RIGHT_24=1521,20
PRIMER_LEFT_24_TM=60.195
PRIMER_RIGHT_24_TM=59.018
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=808
PRIMER_PAIR_25_PENALTY=1.179165
PRIMER_LEFT_25_PENALTY=0.196903
PRIMER_RIGHT_25_PENALTY=0.982262
PRIMER_LEFT_25_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_25_SEQUENCE=GTGCCTACAGACCCATTCCT
PRIMER_LEFT_25=713,20
PRIMER_RIGHT_25=1521,20
PRIMER_LEFT_25_TM=59.803
PRIMER_RIGHT_25_TM=59.018
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=809
PRIMER_PAIR_26_PENALTY=1.186127
PRIMER_LEFT_26_PENALTY=0.716126
PRIMER_RIGHT_26_PENALTY=0.470001
PRIMER_LEFT_26_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_26_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_26=715,20
PRIMER_RIGHT_26=1270,20
PRIMER_LEFT_26_TM=59.284
PRIMER_RIGHT_26_TM=59.530
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=60.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=556
PRIMER_PAIR_27_PENALTY=1.285476
PRIMER_LEFT_27_PENALTY=0.716126
PRIMER_RIGHT_27_PENALTY=0.569350
PRIMER_LEFT_27_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_27_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_27=715,20
PRIMER_RIGHT_27=1359,20
PRIMER_LEFT_27_TM=59.284
PRIMER_RIGHT_27_TM=60.569
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=645
PRIMER_PAIR_28_PENALTY=1.319286
PRIMER_LEFT_28_PENALTY=0.716126
PRIMER_RIGHT_28_PENALTY=0.603160
PRIMER_LEFT_28_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_28_SEQUENCE=CATTCCTTCAATCCGCAGTC
PRIMER_LEFT_28=715,20
PRIMER_RIGHT_28=1608,20
PRIMER_LEFT_28_TM=59.284
PRIMER_RIGHT_28_TM=60.603
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.7000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=894
PRIMER_PAIR_29_PENALTY=1.358022
PRIMER_LEFT_29_PENALTY=0.912701
PRIMER_RIGHT_29_PENALTY=0.445321
PRIMER_LEFT_29_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_29_SEQUENCE=AGAAGCTGAGGTCCGAGATG
PRIMER_LEFT_29=716,20
PRIMER_RIGHT_29=1196,20
PRIMER_LEFT_29_TM=60.913
PRIMER_RIGHT_29_TM=59.555
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=481
PRIMER_PAIR_30_PENALTY=1.382701
PRIMER_LEFT_30_PENALTY=0.912701
PRIMER_RIGHT_30_PENALTY=0.470001
PRIMER_LEFT_30_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_30_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_30=716,20
PRIMER_RIGHT_30=1270,20
PRIMER_LEFT_30_TM=60.913
PRIMER_RIGHT_30_TM=59.530
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=60.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=555
PRIMER_PAIR_31_PENALTY=1.396638
PRIMER_LEFT_31_PENALTY=0.195358
PRIMER_RIGHT_31_PENALTY=1.201280
PRIMER_LEFT_31_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_31_SEQUENCE=CCGAACTAGACTAAGCAGCGA
PRIMER_LEFT_31=714,20
PRIMER_RIGHT_31=1427,21
PRIMER_LEFT_31_TM=60.195
PRIMER_RIGHT_31_TM=59.799
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=714
PRIMER_PAIR_32_PENALTY=1.398183
PRIMER_LEFT_32_PENALTY=0.196903
PRIMER_RIGHT_32_PENALTY=1.201280
PRIMER_LEFT_32_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_32_SEQUENCE=CCGAACTAGACTAAGCAGCGA
PRIMER_LEFT_32=713,20
PRIMER_RIGHT_32=1427,21
PRIMER_LEFT_32_TM=59.803
PRIMER_RIGHT_32_TM=59.799
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=715
PRIMER_PAIR_33_PENALTY=1.409974
PRIMER_LEFT_33_PENALTY=0.716126
PRIMER_RIGHT_33_PENALTY=0.693848
PRIMER_LEFT_33_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_33_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_33=715,20
PRIMER_RIGHT_33=1358,20
PRIMER_LEFT_33_TM=59.284
PRIMER_RIGHT_33_TM=60.694
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.7000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=644
PRIMER_PAIR_34_PENALTY=1.427243
PRIMER_LEFT_34_PENALTY=0.195358
PRIMER_RIGHT_34_PENALTY=1.231885
PRIMER_LEFT_34_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_34_SEQUENCE=AGAAGAAGCTGAGGTCCGAG
PRIMER_LEFT_34=714,20
PRIMER_RIGHT_34=1199,20
PRIMER_LEFT_34_TM=60.195
PRIMER_RIGHT_34_TM=58.768
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.4000
PRIMER_RIGHT_34_END_STABILITY=9.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=486
PRIMER_PAIR_35_PENALTY=1.428788
PRIMER_LEFT_35_PENALTY=0.196903
PRIMER_RIGHT_35_PENALTY=1.231885
PRIMER_LEFT_35_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_35_SEQUENCE=AGAAGAAGCTGAGGTCCGAG
PRIMER_LEFT_35=713,20
PRIMER_RIGHT_35=1199,20
PRIMER_LEFT_35_TM=59.803
PRIMER_RIGHT_35_TM=58.768
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=487
PRIMER_PAIR_36_PENALTY=1.482051
PRIMER_LEFT_36_PENALTY=0.912701
PRIMER_RIGHT_36_PENALTY=0.569350
PRIMER_LEFT_36_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_36_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_36=716,20
PRIMER_RIGHT_36=1359,20
PRIMER_LEFT_36_TM=60.913
PRIMER_RIGHT_36_TM=60.569
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=644
PRIMER_PAIR_37_PENALTY=1.491844
PRIMER_LEFT_37_PENALTY=0.716126
PRIMER_RIGHT_37_PENALTY=0.775718
PRIMER_LEFT_37_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_37_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_37=715,20
PRIMER_RIGHT_37=1599,20
PRIMER_LEFT_37_TM=59.284
PRIMER_RIGHT_37_TM=60.776
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=885
PRIMER_PAIR_38_PENALTY=1.515860
PRIMER_LEFT_38_PENALTY=0.912701
PRIMER_RIGHT_38_PENALTY=0.603160
PRIMER_LEFT_38_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_38_SEQUENCE=CATTCCTTCAATCCGCAGTC
PRIMER_LEFT_38=716,20
PRIMER_RIGHT_38=1608,20
PRIMER_LEFT_38_TM=60.913
PRIMER_RIGHT_38_TM=60.603
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=893
PRIMER_PAIR_39_PENALTY=1.606549
PRIMER_LEFT_39_PENALTY=0.912701
PRIMER_RIGHT_39_PENALTY=0.693848
PRIMER_LEFT_39_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_39_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_39=716,20
PRIMER_RIGHT_39=1358,20
PRIMER_LEFT_39_TM=60.913
PRIMER_RIGHT_39_TM=60.694
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=643
PRIMER_PAIR_40_PENALTY=1.639911
PRIMER_LEFT_40_PENALTY=0.716126
PRIMER_RIGHT_40_PENALTY=0.923785
PRIMER_LEFT_40_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_40_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_40=715,20
PRIMER_RIGHT_40=1523,20
PRIMER_LEFT_40_TM=59.284
PRIMER_RIGHT_40_TM=60.924
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.7000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=809
PRIMER_PAIR_41_PENALTY=1.688419
PRIMER_LEFT_41_PENALTY=0.912701
PRIMER_RIGHT_41_PENALTY=0.775718
PRIMER_LEFT_41_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_41_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_41=716,20
PRIMER_RIGHT_41=1599,20
PRIMER_LEFT_41_TM=60.913
PRIMER_RIGHT_41_TM=60.776
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=884
PRIMER_PAIR_42_PENALTY=1.698388
PRIMER_LEFT_42_PENALTY=0.716126
PRIMER_RIGHT_42_PENALTY=0.982262
PRIMER_LEFT_42_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_42_SEQUENCE=GTGCCTACAGACCCATTCCT
PRIMER_LEFT_42=715,20
PRIMER_RIGHT_42=1521,20
PRIMER_LEFT_42_TM=59.284
PRIMER_RIGHT_42_TM=59.018
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.7000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=807
PRIMER_PAIR_43_PENALTY=1.728223
PRIMER_LEFT_43_PENALTY=0.195358
PRIMER_RIGHT_43_PENALTY=1.532866
PRIMER_LEFT_43_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_43_SEQUENCE=ACCTCAAGGGACTTCTGCTC
PRIMER_LEFT_43=714,20
PRIMER_RIGHT_43=1694,20
PRIMER_LEFT_43_TM=60.195
PRIMER_RIGHT_43_TM=58.467
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=981
PRIMER_PAIR_44_PENALTY=1.729769
PRIMER_LEFT_44_PENALTY=0.196903
PRIMER_RIGHT_44_PENALTY=1.532866
PRIMER_LEFT_44_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_44_SEQUENCE=ACCTCAAGGGACTTCTGCTC
PRIMER_LEFT_44=713,20
PRIMER_RIGHT_44=1694,20
PRIMER_LEFT_44_TM=59.803
PRIMER_RIGHT_44_TM=58.467
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=982
PRIMER_PAIR_45_PENALTY=1.836485
PRIMER_LEFT_45_PENALTY=0.912701
PRIMER_RIGHT_45_PENALTY=0.923785
PRIMER_LEFT_45_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_45_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_45=716,20
PRIMER_RIGHT_45=1523,20
PRIMER_LEFT_45_TM=60.913
PRIMER_RIGHT_45_TM=60.924
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=808
PRIMER_PAIR_46_PENALTY=1.850008
PRIMER_LEFT_46_PENALTY=0.195358
PRIMER_RIGHT_46_PENALTY=1.654650
PRIMER_LEFT_46_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_46_SEQUENCE=GAGAAGAAGCTGAGGTCCGAG
PRIMER_LEFT_46=714,20
PRIMER_RIGHT_46=1200,21
PRIMER_LEFT_46_TM=60.195
PRIMER_RIGHT_46_TM=60.655
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=57.143
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.4000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=487
PRIMER_PAIR_47_PENALTY=1.851553
PRIMER_LEFT_47_PENALTY=0.196903
PRIMER_RIGHT_47_PENALTY=1.654650
PRIMER_LEFT_47_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_47_SEQUENCE=GAGAAGAAGCTGAGGTCCGAG
PRIMER_LEFT_47=713,20
PRIMER_RIGHT_47=1200,21
PRIMER_LEFT_47_TM=59.803
PRIMER_RIGHT_47_TM=60.655
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=57.143
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=488
PRIMER_PAIR_48_PENALTY=1.879162
PRIMER_LEFT_48_PENALTY=0.195358
PRIMER_RIGHT_48_PENALTY=1.683804
PRIMER_LEFT_48_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_48_SEQUENCE=GGTGCCTACAGACCCATTCC
PRIMER_LEFT_48=714,20
PRIMER_RIGHT_48=1522,20
PRIMER_LEFT_48_TM=60.195
PRIMER_RIGHT_48_TM=61.684
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=60.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=809
PRIMER_PAIR_49_PENALTY=1.880707
PRIMER_LEFT_49_PENALTY=0.196903
PRIMER_RIGHT_49_PENALTY=1.683804
PRIMER_LEFT_49_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_49_SEQUENCE=GGTGCCTACAGACCCATTCC
PRIMER_LEFT_49=713,20
PRIMER_RIGHT_49=1522,20
PRIMER_LEFT_49_TM=59.803
PRIMER_RIGHT_49_TM=61.684
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=60.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=8.1000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=810
=
SEQUENCE_ID=MAGIC.329_3_14535649_14535785_bur-0_ws-0_2514_16150_TRUE
SEQUENCE_TARGET=950,236
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAATTTATCTTCATTANNNNNNNNNNNNNNNNNNTGATTACAAAGGGGNNTNTAATCTCTTGTGACACACATTAGGGTTTTANTNNNANNNNAAGAAAGAATTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATGTGTCTTTNTGAGTATCAAANTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCGCATCTAANCTNACTNATCCGTATNTTTTGAGAGNAAGGCTTGTTTTTCTTGNTCCCNAACAATGTCGTTGTTTTNNNNNNNNNNNNNNNNNTTTTTATGTAAAGACATGNNTNG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4493375_4493461_can-0_kn-0_40271_12822_TRUE
SEQUENCE_TARGET=950,186
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_9612629_9613227_sf-2_zu-0_596292_12341_TRUE
SEQUENCE_TARGET=950,698
SEQUENCE_TEMPLATE=AGAGAGAGTAGGAGNCNNNNNNNNNNNNNNNNNNNNNNNNGTNTAACTTTGTGANTNTATAATTGNNNNNNNNNNNNNNNNNNNNNNNNAGAGAACTAATNANCAGAGCTACCTCGCCANCTTGNAATCCCNGAAAGAAACCCAGTTCACCTCTTGTGGGTATACAATACACGGAACANNNNNNNNNNNNNNNNNNNNNNNNNNTTATTCTNTGTNNACAACGATTANGACAGGNNGAGNAAATAGGTCGANATATACCCATAAAGTTAGGCAGAATCAATAGCGATCCCCTAGAGANAGACCATGNTGAACGAGNNTTCAAGTATAAGTCCTTCCCAAGCATACATGTAATGTGTAGGTNGACACAACAATANTCATCACACNTATAAAACCCNTCTTCNGGATTTATTTTNCCCTCACAAGCTTCACACCAATANNTCATGCTACTTGTCTTCTTCCCATAANAAAGAGGGAGTATGTGTTTATCATNCNTATACCTNACTTNTTGNGGTAAAGTAGCACATTTNAAACACAAAGCANANTCACACNCNTNCTCAATGCAATTGAAGGTTTCCTTGGTGTAATAACCTCGCGCTTCGGCGCAAACCGAACANTTTCTTTNCTCNTNTNGTNTGGATGTTAGGAATAAAGGATGCNTATGACTTGGATGAAGTAAAGGCTCCGAAATTNTGGCGCACTGCACATGTAGCCTAAAATCGCAATTTCTTTCACTGCACTNATAGAANAAACCAGCTATACACAACGAAGGACAAGCTGAACANNTATCACGATCAGAATTANNNNNNNNNNCGACAGNTCTGAGAGGGTTATCTGTNACTAAAGTGAGTNGATGNGGATGTATCGGGTGATNTNANCTGCGAGAAAGNTTTGCACATTCTTCNTGTAGAATGAAATCACATTGCAANCAAGAGTAGGAGTTACCAAAATAAATNGGCATGATGCATGCTTGACANAGCTTATTCTCGTCGTAATCTTTGNNNGTGTTCTCATNAAGTGTCAAATGATGATNTNCATGACTGAAATNTTGTATGANTCCATCGCTTATNGTCANGAATGGCTCAAGNTCTTCCACTTCTTCTTCNNACTCTCCTTCACGTNCTATACCATCCCAAACATTTGTTTGAGTGGCNCATTTTGAATGNACCGCATAAANACAACCNTTCTTGATGCAATGATANCCCCCGTANTCATTGTTAATCTTCCGGCGGCAAANACCACAAATNCNATCTCCTTTTGGGAAAGAATGGGTAAAANAGATANAATGGNNGTGNCGAGAAATCCGTATGACATGAGGTAAGCTNAAACANCTTTGATGNACGATAAAGTNGCAAGGNGGNCATATATAGAAAANCAANTTTGAATGTTTNAAGGCNCANAGGCTGCAAGTTNNGGNATCCNGACTTGGAAANANAGCAAGTGTATGCNCATGCCACTTGGGTTGGTCGATAGACAAGACTNGTGATTTCTTNGCACATGTAAATTTGAGCCCAAAATCGNAAGNAGAACAAAAATAAAACATACTTGTGGTATCGTCATGGAGAANTTCGTGACAGCAATAGCATTGCNNNANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5875618_5876132_oy-0_no-0_57081_38141_TRUE
SEQUENCE_TARGET=950,614
SEQUENCE_TEMPLATE=TACCCAAAAACTTCTTATTANTAAAGAATNTTTCTNANAAAAAAANNANAAATTANTTAACAAAACTAAGAAGAGGCGTTACCAACACTTACCACATTCTTACACCTTGTTTTNCATANCCATAATAATAAACCAAACAGATGTATTGCTCAACAAAGTACATTGCTCGTCACTTGGANNNNNNNNNNNNNNNNNNNNNNNCCCATAAGAACATAAGANCACNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAATTAGAATACATATTCTAAATTCCGTATAACNTAATNAAAAANACATATTNCTAATTTGNAGATTNAGTTTCCTTATTTTTCTCTACTCGATTCCTGNTTTTATGGAATTCTAAATTTCAAAGAATGTAGTTTCTAATTTTAGTAGAACANATTTTANTTTAATTNTGCTGAACATATGATAAAGTGTAAAACATNTATTCTACNAATCGTAGAACATAGAGTAATTTNTAGAATACATATTCTAAATTTCGTAGAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGANNTCTGAAATATTCTAACNAGTTTAGATACTCTGATGTTGTAANGNNNNNNNNNNNNNNNNTNTCACAAAACGGTTTTGAGATGGTGAAGAAGAATCTTTCCATATCTGTGAGCTTCTCTCGTTTTGGTTACGGTCAGGTTCTGTGGAAAGACACTCTATTGGCATAGCTTCTTCTTCTAACTCTCTATGGAATAAACGTAAACCTAGANTTNCACCTCTTTGNAACAGTTTGAAAGTNNAGAAACNTCNAGCTNTAANTCCATTGGCATNACAGAAACTTCTCCATCCTCNTTTGATGTAAGTAGTTNGACATGANTTNTTTCGTTTCAANTCTANAGTCNATGATNTNCCTTTNNNNNNNNNNAGAATCATCTCTCCACACATTTTTTCTANACCATTTGCCCTCACAAANNNNNNANTTAAATCCAACTAGTATTAGTTAAACAGATTNTCTCTACTTACATATTCTCTTTTTTAATATAAGTTANAGAGACGAAAGAACACAACTCACCGAGGTTGTTCTTATTGTCTAAAGACACNTANTNTCACAACAACTAGGTNCCAACATTGTTANGTGGAAAGTCATGTNTCTCTCTTGTCTGAAAACAATGATGTCGCCGAGTCGAAGATCATGTGCGAGAGCAAAATCTTTCCAANCGTTGGTAAGTCTCTGGCCATCTNTTTTCACCTTCTAGGTTTTNTTGGATGCATCTGACCTTAACNNTGNCGTCTTCTGCTNATTTCTTCCTTCTATATTCTCAAANNAGAAGGCTAAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCATTATTCTCAATGATCNTCTCAGAGGGACATATNCAGAGTAGAGAAAGATTTACNAAGTGGCNGTGAAAGCCAGGAAAAAGNGGCTTGAANAAATGTTGATTCGCCATTATCAAAGCTCCAGAATCTCTCAGCCTTTACACTGCAATCTCAAGNAGGAAANAGTATAATAAANTAATAAACACAACGTTCACTTCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACTAAAGNCTGTTCGGTTTCCACTNNGTGANTGAACAATNAACATATANGAACTCNANAGCTTTANAAAAAGTAAAACCAGATTACTTTTTTATATAAAAACAAAACCATCAAANTNTTTGATNGTGTNATTATGNNNNTTACATGCTTTCATATTAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.283201
PRIMER_LEFT_0_PENALTY=0.108684
PRIMER_RIGHT_0_PENALTY=0.174518
PRIMER_LEFT_0_SEQUENCE=CGTTTTGGTTACGGTCAGGT
PRIMER_RIGHT_0_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_0=911,20
PRIMER_RIGHT_0=1727,20
PRIMER_LEFT_0_TM=59.891
PRIMER_RIGHT_0_TM=60.175
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=10.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=817
PRIMER_PAIR_1_PENALTY=0.719648
PRIMER_LEFT_1_PENALTY=0.545130
PRIMER_RIGHT_1_PENALTY=0.174518
PRIMER_LEFT_1_SEQUENCE=ACGGTCAGGTTCTGTGGAAA
PRIMER_RIGHT_1_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_1=921,20
PRIMER_RIGHT_1=1727,20
PRIMER_LEFT_1_TM=60.545
PRIMER_RIGHT_1_TM=60.175
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=807
PRIMER_PAIR_2_PENALTY=1.039604
PRIMER_LEFT_2_PENALTY=0.865086
PRIMER_RIGHT_2_PENALTY=0.174518
PRIMER_LEFT_2_SEQUENCE=TTACGGTCAGGTTCTGTGGA
PRIMER_RIGHT_2_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_2=919,20
PRIMER_RIGHT_2=1727,20
PRIMER_LEFT_2_TM=59.135
PRIMER_RIGHT_2_TM=60.175
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=809
PRIMER_PAIR_3_PENALTY=1.087674
PRIMER_LEFT_3_PENALTY=0.913157
PRIMER_RIGHT_3_PENALTY=0.174518
PRIMER_LEFT_3_SEQUENCE=TCGTTTTGGTTACGGTCAGG
PRIMER_RIGHT_3_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_3=910,20
PRIMER_RIGHT_3=1727,20
PRIMER_LEFT_3_TM=60.913
PRIMER_RIGHT_3_TM=60.175
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=818
PRIMER_PAIR_4_PENALTY=1.194806
PRIMER_LEFT_4_PENALTY=1.020289
PRIMER_RIGHT_4_PENALTY=0.174518
PRIMER_LEFT_4_SEQUENCE=GTTACGGTCAGGTTCTGTGGA
PRIMER_RIGHT_4_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_4=918,21
PRIMER_RIGHT_4=1727,20
PRIMER_LEFT_4_TM=60.020
PRIMER_RIGHT_4_TM=60.175
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=810
PRIMER_PAIR_5_PENALTY=1.270259
PRIMER_LEFT_5_PENALTY=1.095742
PRIMER_RIGHT_5_PENALTY=0.174518
PRIMER_LEFT_5_SEQUENCE=CGGTTTTGAGATGGTGAAGAA
PRIMER_RIGHT_5_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_5=862,21
PRIMER_RIGHT_5=1727,20
PRIMER_LEFT_5_TM=60.096
PRIMER_RIGHT_5_TM=60.175
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=10.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=866
PRIMER_PAIR_6_PENALTY=1.328106
PRIMER_LEFT_6_PENALTY=1.153589
PRIMER_RIGHT_6_PENALTY=0.174518
PRIMER_LEFT_6_SEQUENCE=TCTCGTTTTGGTTACGGTCAG
PRIMER_RIGHT_6_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_6=908,21
PRIMER_RIGHT_6=1727,20
PRIMER_LEFT_6_TM=60.154
PRIMER_RIGHT_6_TM=60.175
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=10.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=820
PRIMER_PAIR_7_PENALTY=1.385898
PRIMER_LEFT_7_PENALTY=0.108684
PRIMER_RIGHT_7_PENALTY=1.277215
PRIMER_LEFT_7_SEQUENCE=CGTTTTGGTTACGGTCAGGT
PRIMER_RIGHT_7_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_7=911,20
PRIMER_RIGHT_7=1724,20
PRIMER_LEFT_7_TM=59.891
PRIMER_RIGHT_7_TM=58.723
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=814
PRIMER_PAIR_8_PENALTY=1.479520
PRIMER_LEFT_8_PENALTY=1.305002
PRIMER_RIGHT_8_PENALTY=0.174518
PRIMER_LEFT_8_SEQUENCE=CGGTTTTGAGATGGTGAAGA
PRIMER_RIGHT_8_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_8=862,20
PRIMER_RIGHT_8=1727,20
PRIMER_LEFT_8_TM=58.695
PRIMER_RIGHT_8_TM=60.175
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=10.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=866
PRIMER_PAIR_9_PENALTY=1.591502
PRIMER_LEFT_9_PENALTY=1.416984
PRIMER_RIGHT_9_PENALTY=0.174518
PRIMER_LEFT_9_SEQUENCE=ACGGTTTTGAGATGGTGAAGA
PRIMER_RIGHT_9_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_9=861,21
PRIMER_RIGHT_9=1727,20
PRIMER_LEFT_9_TM=59.583
PRIMER_RIGHT_9_TM=60.175
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=10.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=867
PRIMER_PAIR_10_PENALTY=1.668930
PRIMER_LEFT_10_PENALTY=1.494413
PRIMER_RIGHT_10_PENALTY=0.174518
PRIMER_LEFT_10_SEQUENCE=TTGGTTACGGTCAGGTTCTGT
PRIMER_RIGHT_10_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_10=915,21
PRIMER_RIGHT_10=1727,20
PRIMER_LEFT_10_TM=59.506
PRIMER_RIGHT_10_TM=60.175
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=10.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=813
PRIMER_PAIR_11_PENALTY=1.709408
PRIMER_LEFT_11_PENALTY=1.534890
PRIMER_RIGHT_11_PENALTY=0.174518
PRIMER_LEFT_11_SEQUENCE=TACGGTCAGGTTCTGTGGAAA
PRIMER_RIGHT_11_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_11=920,21
PRIMER_RIGHT_11=1727,20
PRIMER_LEFT_11_TM=60.535
PRIMER_RIGHT_11_TM=60.175
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=10.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=808
PRIMER_PAIR_12_PENALTY=1.730555
PRIMER_LEFT_12_PENALTY=1.556038
PRIMER_RIGHT_12_PENALTY=0.174518
PRIMER_LEFT_12_SEQUENCE=TGTGAGCTTCTCTCGTTTTGG
PRIMER_RIGHT_12_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_12=898,21
PRIMER_RIGHT_12=1727,20
PRIMER_LEFT_12_TM=60.556
PRIMER_RIGHT_12_TM=60.175
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.8000
PRIMER_RIGHT_12_END_STABILITY=10.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=830
PRIMER_PAIR_13_PENALTY=1.822345
PRIMER_LEFT_13_PENALTY=0.545130
PRIMER_RIGHT_13_PENALTY=1.277215
PRIMER_LEFT_13_SEQUENCE=ACGGTCAGGTTCTGTGGAAA
PRIMER_RIGHT_13_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_13=921,20
PRIMER_RIGHT_13=1724,20
PRIMER_LEFT_13_TM=60.545
PRIMER_RIGHT_13_TM=58.723
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=804
PRIMER_PAIR_14_PENALTY=1.892126
PRIMER_LEFT_14_PENALTY=1.717608
PRIMER_RIGHT_14_PENALTY=0.174518
PRIMER_LEFT_14_SEQUENCE=CTCGTTTTGGTTACGGTCAG
PRIMER_RIGHT_14_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_14=909,20
PRIMER_RIGHT_14=1727,20
PRIMER_LEFT_14_TM=58.282
PRIMER_RIGHT_14_TM=60.175
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=10.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=819
PRIMER_PAIR_15_PENALTY=1.946061
PRIMER_LEFT_15_PENALTY=1.771543
PRIMER_RIGHT_15_PENALTY=0.174518
PRIMER_LEFT_15_SEQUENCE=TCTCTCGTTTTGGTTACGGTC
PRIMER_RIGHT_15_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_15=906,21
PRIMER_RIGHT_15=1727,20
PRIMER_LEFT_15_TM=59.228
PRIMER_RIGHT_15_TM=60.175
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=10.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=822
PRIMER_PAIR_16_PENALTY=2.063229
PRIMER_LEFT_16_PENALTY=1.888711
PRIMER_RIGHT_16_PENALTY=0.174518
PRIMER_LEFT_16_SEQUENCE=TGAGCTTCTCTCGTTTTGGTT
PRIMER_RIGHT_16_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_16=900,21
PRIMER_RIGHT_16=1727,20
PRIMER_LEFT_16_TM=59.111
PRIMER_RIGHT_16_TM=60.175
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=10.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=828
PRIMER_PAIR_17_PENALTY=2.087423
PRIMER_LEFT_17_PENALTY=1.912906
PRIMER_RIGHT_17_PENALTY=0.174518
PRIMER_LEFT_17_SEQUENCE=AACGGTTTTGAGATGGTGAAG
PRIMER_RIGHT_17_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_17=860,21
PRIMER_RIGHT_17=1727,20
PRIMER_LEFT_17_TM=59.087
PRIMER_RIGHT_17_TM=60.175
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=10.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=868
PRIMER_PAIR_18_PENALTY=2.115811
PRIMER_LEFT_18_PENALTY=1.941293
PRIMER_RIGHT_18_PENALTY=0.174518
PRIMER_LEFT_18_SEQUENCE=GTTACGGTCAGGTTCTGTGG
PRIMER_RIGHT_18_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_18=918,20
PRIMER_RIGHT_18=1727,20
PRIMER_LEFT_18_TM=58.059
PRIMER_RIGHT_18_TM=60.175
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=10.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=810
PRIMER_PAIR_19_PENALTY=2.115811
PRIMER_LEFT_19_PENALTY=1.941293
PRIMER_RIGHT_19_PENALTY=0.174518
PRIMER_LEFT_19_SEQUENCE=GGTTACGGTCAGGTTCTGTG
PRIMER_RIGHT_19_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_19=917,20
PRIMER_RIGHT_19=1727,20
PRIMER_LEFT_19_TM=58.059
PRIMER_RIGHT_19_TM=60.175
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=10.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=811
PRIMER_PAIR_20_PENALTY=2.115811
PRIMER_LEFT_20_PENALTY=1.941293
PRIMER_RIGHT_20_PENALTY=0.174518
PRIMER_LEFT_20_SEQUENCE=TGGTTACGGTCAGGTTCTGT
PRIMER_RIGHT_20_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_20=916,20
PRIMER_RIGHT_20=1727,20
PRIMER_LEFT_20_TM=58.059
PRIMER_RIGHT_20_TM=60.175
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=10.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=812
PRIMER_PAIR_21_PENALTY=2.126338
PRIMER_LEFT_21_PENALTY=1.951820
PRIMER_RIGHT_21_PENALTY=0.174518
PRIMER_LEFT_21_SEQUENCE=AACGGTTTTGAGATGGTGAA
PRIMER_RIGHT_21_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_21=860,20
PRIMER_RIGHT_21=1727,20
PRIMER_LEFT_21_TM=58.048
PRIMER_RIGHT_21_TM=60.175
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=10.2000
PRIMER_PAIR_21_COMPL_ANY=2.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=868
PRIMER_PAIR_22_PENALTY=2.142301
PRIMER_LEFT_22_PENALTY=0.865086
PRIMER_RIGHT_22_PENALTY=1.277215
PRIMER_LEFT_22_SEQUENCE=TTACGGTCAGGTTCTGTGGA
PRIMER_RIGHT_22_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_22=919,20
PRIMER_RIGHT_22=1724,20
PRIMER_LEFT_22_TM=59.135
PRIMER_RIGHT_22_TM=58.723
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=806
PRIMER_PAIR_23_PENALTY=2.163602
PRIMER_LEFT_23_PENALTY=1.989084
PRIMER_RIGHT_23_PENALTY=0.174518
PRIMER_LEFT_23_SEQUENCE=TGGTTACGGTCAGGTTCTGTG
PRIMER_RIGHT_23_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_23=916,21
PRIMER_RIGHT_23=1727,20
PRIMER_LEFT_23_TM=60.989
PRIMER_RIGHT_23_TM=60.175
PRIMER_LEFT_23_GC_PERCENT=52.381
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=10.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=812
PRIMER_PAIR_24_PENALTY=2.190371
PRIMER_LEFT_24_PENALTY=0.913157
PRIMER_RIGHT_24_PENALTY=1.277215
PRIMER_LEFT_24_SEQUENCE=TCGTTTTGGTTACGGTCAGG
PRIMER_RIGHT_24_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_24=910,20
PRIMER_RIGHT_24=1724,20
PRIMER_LEFT_24_TM=60.913
PRIMER_RIGHT_24_TM=58.723
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=815
PRIMER_PAIR_25_PENALTY=2.246201
PRIMER_LEFT_25_PENALTY=2.071684
PRIMER_RIGHT_25_PENALTY=0.174518
PRIMER_LEFT_25_SEQUENCE=GTGAGCTTCTCTCGTTTTGGTT
PRIMER_RIGHT_25_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_25=899,22
PRIMER_RIGHT_25=1727,20
PRIMER_LEFT_25_TM=59.928
PRIMER_RIGHT_25_TM=60.175
PRIMER_LEFT_25_GC_PERCENT=45.455
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=10.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=829
PRIMER_PAIR_26_PENALTY=2.284184
PRIMER_LEFT_26_PENALTY=2.109666
PRIMER_RIGHT_26_PENALTY=0.174518
PRIMER_LEFT_26_SEQUENCE=CTTCTCTCGTTTTGGTTACGG
PRIMER_RIGHT_26_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_26=904,21
PRIMER_RIGHT_26=1727,20
PRIMER_LEFT_26_TM=58.890
PRIMER_RIGHT_26_TM=60.175
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.9000
PRIMER_RIGHT_26_END_STABILITY=10.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=824
PRIMER_PAIR_27_PENALTY=2.295065
PRIMER_LEFT_27_PENALTY=2.120547
PRIMER_RIGHT_27_PENALTY=0.174518
PRIMER_LEFT_27_SEQUENCE=TTCTCTCGTTTTGGTTACGG
PRIMER_RIGHT_27_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_27=905,20
PRIMER_RIGHT_27=1727,20
PRIMER_LEFT_27_TM=57.879
PRIMER_RIGHT_27_TM=60.175
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.9000
PRIMER_RIGHT_27_END_STABILITY=10.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=823
PRIMER_PAIR_28_PENALTY=2.295274
PRIMER_LEFT_28_PENALTY=0.108684
PRIMER_RIGHT_28_PENALTY=2.186590
PRIMER_LEFT_28_SEQUENCE=CGTTTTGGTTACGGTCAGGT
PRIMER_RIGHT_28_SEQUENCE=TGCAGTGTAAAGGCTGAGAGA
PRIMER_LEFT_28=911,20
PRIMER_RIGHT_28=1752,21
PRIMER_LEFT_28_TM=59.891
PRIMER_RIGHT_28_TM=58.813
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=842
PRIMER_PAIR_29_PENALTY=2.297503
PRIMER_LEFT_29_PENALTY=1.020289
PRIMER_RIGHT_29_PENALTY=1.277215
PRIMER_LEFT_29_SEQUENCE=GTTACGGTCAGGTTCTGTGGA
PRIMER_RIGHT_29_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_29=918,21
PRIMER_RIGHT_29=1724,20
PRIMER_LEFT_29_TM=60.020
PRIMER_RIGHT_29_TM=58.723
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=807
PRIMER_PAIR_30_PENALTY=2.298280
PRIMER_LEFT_30_PENALTY=0.108684
PRIMER_RIGHT_30_PENALTY=2.189596
PRIMER_LEFT_30_SEQUENCE=CGTTTTGGTTACGGTCAGGT
PRIMER_RIGHT_30_SEQUENCE=TTGCAGTGTAAAGGCTGAGAGA
PRIMER_LEFT_30=911,20
PRIMER_RIGHT_30=1753,22
PRIMER_LEFT_30_TM=59.891
PRIMER_RIGHT_30_TM=60.190
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=843
PRIMER_PAIR_31_PENALTY=2.329475
PRIMER_LEFT_31_PENALTY=2.154958
PRIMER_RIGHT_31_PENALTY=0.174518
PRIMER_LEFT_31_SEQUENCE=CGTTTTGGTTACGGTCAGGTT
PRIMER_RIGHT_31_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_31=911,21
PRIMER_RIGHT_31=1727,20
PRIMER_LEFT_31_TM=61.155
PRIMER_RIGHT_31_TM=60.175
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=10.2000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=817
PRIMER_PAIR_32_PENALTY=2.372956
PRIMER_LEFT_32_PENALTY=1.095742
PRIMER_RIGHT_32_PENALTY=1.277215
PRIMER_LEFT_32_SEQUENCE=CGGTTTTGAGATGGTGAAGAA
PRIMER_RIGHT_32_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_32=862,21
PRIMER_RIGHT_32=1724,20
PRIMER_LEFT_32_TM=60.096
PRIMER_RIGHT_32_TM=58.723
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=863
PRIMER_PAIR_33_PENALTY=2.377841
PRIMER_LEFT_33_PENALTY=2.203324
PRIMER_RIGHT_33_PENALTY=0.174518
PRIMER_LEFT_33_SEQUENCE=TCTGTGAGCTTCTCTCGTTTTG
PRIMER_RIGHT_33_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_33=896,22
PRIMER_RIGHT_33=1727,20
PRIMER_LEFT_33_TM=59.797
PRIMER_RIGHT_33_TM=60.175
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.6000
PRIMER_RIGHT_33_END_STABILITY=10.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=832
PRIMER_PAIR_34_PENALTY=2.430803
PRIMER_LEFT_34_PENALTY=1.153589
PRIMER_RIGHT_34_PENALTY=1.277215
PRIMER_LEFT_34_SEQUENCE=TCTCGTTTTGGTTACGGTCAG
PRIMER_RIGHT_34_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_34=908,21
PRIMER_RIGHT_34=1724,20
PRIMER_LEFT_34_TM=60.154
PRIMER_RIGHT_34_TM=58.723
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=817
PRIMER_PAIR_35_PENALTY=2.503728
PRIMER_LEFT_35_PENALTY=2.329211
PRIMER_RIGHT_35_PENALTY=0.174518
PRIMER_LEFT_35_SEQUENCE=TGAGCTTCTCTCGTTTTGGT
PRIMER_RIGHT_35_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_35=900,20
PRIMER_RIGHT_35=1727,20
PRIMER_LEFT_35_TM=57.671
PRIMER_RIGHT_35_TM=60.175
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=10.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=828
PRIMER_PAIR_36_PENALTY=2.503728
PRIMER_LEFT_36_PENALTY=2.329211
PRIMER_RIGHT_36_PENALTY=0.174518
PRIMER_LEFT_36_SEQUENCE=GTGAGCTTCTCTCGTTTTGG
PRIMER_RIGHT_36_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_36=899,20
PRIMER_RIGHT_36=1727,20
PRIMER_LEFT_36_TM=57.671
PRIMER_RIGHT_36_TM=60.175
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.8000
PRIMER_RIGHT_36_END_STABILITY=10.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=829
PRIMER_PAIR_37_PENALTY=2.533071
PRIMER_LEFT_37_PENALTY=2.358553
PRIMER_RIGHT_37_PENALTY=0.174518
PRIMER_LEFT_37_SEQUENCE=ACGGTTTTGAGATGGTGAAG
PRIMER_RIGHT_37_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_37=861,20
PRIMER_RIGHT_37=1727,20
PRIMER_LEFT_37_TM=57.641
PRIMER_RIGHT_37_TM=60.175
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=10.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=867
PRIMER_PAIR_38_PENALTY=2.560459
PRIMER_LEFT_38_PENALTY=2.385942
PRIMER_RIGHT_38_PENALTY=0.174518
PRIMER_LEFT_38_SEQUENCE=TTTTGGTTACGGTCAGGTTCT
PRIMER_RIGHT_38_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_38=913,21
PRIMER_RIGHT_38=1727,20
PRIMER_LEFT_38_TM=58.614
PRIMER_RIGHT_38_TM=60.175
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=10.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=815
PRIMER_PAIR_39_PENALTY=2.582217
PRIMER_LEFT_39_PENALTY=1.305002
PRIMER_RIGHT_39_PENALTY=1.277215
PRIMER_LEFT_39_SEQUENCE=CGGTTTTGAGATGGTGAAGA
PRIMER_RIGHT_39_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_39=862,20
PRIMER_RIGHT_39=1724,20
PRIMER_LEFT_39_TM=58.695
PRIMER_RIGHT_39_TM=58.723
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=863
PRIMER_PAIR_40_PENALTY=2.585257
PRIMER_LEFT_40_PENALTY=2.410740
PRIMER_RIGHT_40_PENALTY=0.174518
PRIMER_LEFT_40_SEQUENCE=GTGAGCTTCTCTCGTTTTGGT
PRIMER_RIGHT_40_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_40=899,21
PRIMER_RIGHT_40=1727,20
PRIMER_LEFT_40_TM=58.589
PRIMER_RIGHT_40_TM=60.175
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=10.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=829
PRIMER_PAIR_41_PENALTY=2.586936
PRIMER_LEFT_41_PENALTY=0.108684
PRIMER_RIGHT_41_PENALTY=2.478252
PRIMER_LEFT_41_SEQUENCE=CGTTTTGGTTACGGTCAGGT
PRIMER_RIGHT_41_SEQUENCE=GATTGCAGTGTAAAGGCTGAGA
PRIMER_LEFT_41=911,20
PRIMER_RIGHT_41=1755,22
PRIMER_LEFT_41_TM=59.891
PRIMER_RIGHT_41_TM=59.522
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=845
PRIMER_PAIR_42_PENALTY=2.586936
PRIMER_LEFT_42_PENALTY=0.108684
PRIMER_RIGHT_42_PENALTY=2.478252
PRIMER_LEFT_42_SEQUENCE=CGTTTTGGTTACGGTCAGGT
PRIMER_RIGHT_42_SEQUENCE=GAGATTGCAGTGTAAAGGCTGA
PRIMER_LEFT_42=911,20
PRIMER_RIGHT_42=1757,22
PRIMER_LEFT_42_TM=59.891
PRIMER_RIGHT_42_TM=59.522
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=847
PRIMER_PAIR_43_PENALTY=2.596911
PRIMER_LEFT_43_PENALTY=2.422393
PRIMER_RIGHT_43_PENALTY=0.174518
PRIMER_LEFT_43_SEQUENCE=TTTTGGTTACGGTCAGGTTC
PRIMER_RIGHT_43_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_43=913,20
PRIMER_RIGHT_43=1727,20
PRIMER_LEFT_43_TM=57.578
PRIMER_RIGHT_43_TM=60.175
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=10.2000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=815
PRIMER_PAIR_44_PENALTY=2.688349
PRIMER_LEFT_44_PENALTY=2.513831
PRIMER_RIGHT_44_PENALTY=0.174518
PRIMER_LEFT_44_SEQUENCE=TTCTCTCGTTTTGGTTACGGTC
PRIMER_RIGHT_44_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_44=905,22
PRIMER_RIGHT_44=1727,20
PRIMER_LEFT_44_TM=60.514
PRIMER_RIGHT_44_TM=60.175
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=10.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=823
PRIMER_PAIR_45_PENALTY=2.694199
PRIMER_LEFT_45_PENALTY=1.416984
PRIMER_RIGHT_45_PENALTY=1.277215
PRIMER_LEFT_45_SEQUENCE=ACGGTTTTGAGATGGTGAAGA
PRIMER_RIGHT_45_SEQUENCE=GCTTTGATAATGGCGAATCA
PRIMER_LEFT_45=861,21
PRIMER_RIGHT_45=1724,20
PRIMER_LEFT_45_TM=59.583
PRIMER_RIGHT_45_TM=58.723
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=864
PRIMER_PAIR_46_PENALTY=2.700499
PRIMER_LEFT_46_PENALTY=2.525981
PRIMER_RIGHT_46_PENALTY=0.174518
PRIMER_LEFT_46_SEQUENCE=GTTTTGGTTACGGTCAGGTTC
PRIMER_RIGHT_46_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_46=912,21
PRIMER_RIGHT_46=1727,20
PRIMER_LEFT_46_TM=58.474
PRIMER_RIGHT_46_TM=60.175
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=10.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=816
PRIMER_PAIR_47_PENALTY=2.731721
PRIMER_LEFT_47_PENALTY=0.545130
PRIMER_RIGHT_47_PENALTY=2.186590
PRIMER_LEFT_47_SEQUENCE=ACGGTCAGGTTCTGTGGAAA
PRIMER_RIGHT_47_SEQUENCE=TGCAGTGTAAAGGCTGAGAGA
PRIMER_LEFT_47=921,20
PRIMER_RIGHT_47=1752,21
PRIMER_LEFT_47_TM=60.545
PRIMER_RIGHT_47_TM=58.813
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=832
PRIMER_PAIR_48_PENALTY=2.734727
PRIMER_LEFT_48_PENALTY=0.545130
PRIMER_RIGHT_48_PENALTY=2.189596
PRIMER_LEFT_48_SEQUENCE=ACGGTCAGGTTCTGTGGAAA
PRIMER_RIGHT_48_SEQUENCE=TTGCAGTGTAAAGGCTGAGAGA
PRIMER_LEFT_48=921,20
PRIMER_RIGHT_48=1753,22
PRIMER_LEFT_48_TM=60.545
PRIMER_RIGHT_48_TM=60.190
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=833
PRIMER_PAIR_49_PENALTY=2.741480
PRIMER_LEFT_49_PENALTY=2.566962
PRIMER_RIGHT_49_PENALTY=0.174518
PRIMER_LEFT_49_SEQUENCE=GTTTTGGTTACGGTCAGGTTCT
PRIMER_RIGHT_49_SEQUENCE=GGAGCTTTGATAATGGCGAA
PRIMER_LEFT_49=912,22
PRIMER_RIGHT_49=1727,20
PRIMER_LEFT_49_TM=59.433
PRIMER_RIGHT_49_TM=60.175
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=10.2000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=816
=
SEQUENCE_ID=MAGIC.149_3_7850370_7850471_wil-2_ct-1_4154758_177303_TRUE
SEQUENCE_TARGET=950,201
SEQUENCE_TEMPLATE=NNNNNNTGTTGGTATCACCCTGGCAAATGTGGAGTTATTTTTTTCTTATNCAATGATGGAATNGTCATTTGGAACTCAACTAGCTGAAAAATAANTTNAAACTGAAATGTATATGTATAAAAAANAATTTACAAAAACTTTTGCTTTAGTTCTCATCNAGAATAGGCAATAGGTTTCTTAAACATATTTAGTGTCAAGAGTGATTTTATTAAGATTTTAATATTAAATATTTGGATNTATAAAAANTNAAAGATAACGTAACGTGAAAAGGAANTATTAGATTGCGCTATACATATGAAAAATATATACTAAATGATGTATTTGGCGAATCAAGCATCATGGTCTAATACAGAATCATTCCNNNNNNNNNNNNNNGATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTTTNAATATATGGCTTGTTGGGAGGGACTTATCTCACCAAAACAAAGACTNAAGCAAANGTTGGATACAAAGCTAGTGTCGATGAAGAAACGNTATATGCGGTAGCTGCTGAATCTTCTACTGGAACATGGGAAACTAAAGCGATGGGGCATACCAGCCTGGATCGTTTCAAAGATGATGCTACCACATCAAACCCGTTTTGGGAGNAAAAACCTCAATTTATTGCGAATATAATAANTTACCCCTTAGATCTTTTTGATGAATGTTTGGTTACTACCATGTATACCNCGACTGTGGGTAATGTATTTGGGTTCAAAACCATGTTTGTTTTACGTCTAGAGGATATGTGNATCCCTTCNGNTTATACTAAAATGTTCCAAGGACCATCTCATGGNATCCAAGTTGAAAGAGNCAAATTGAACAAGTACTATCTAGAGGATATGTGAATCCCTTCTGCAGTTCATAAACGGCGGTTCAATCGTGTTTTACGATTTGAGATTAAGGTTCTNATAGGACCGGTTCGATTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTNTGTCTTTTNTATANGAGNTGTACACAATAGGTGAAGNTGTACGATATTATAGACGGTGTAGAAGTTGATTTATGGTTTGATTTATTATCTCATTATTGCATGTATANTATTAATATCATGTACACNATATNTTACATTTTCGTACACCATCNTATTATGTAGTTGNACATCGTTGTATATTATCGTTTGTACATCACTTTGTACACTGTNTANACCGTATACCACTTCTTNNATCAATATTATACATTATCGNTGTACACTACTATTTNTACANNNNNNNNNNNNNNNATCGCNTANACCGT
PRIMER_LEFT_NUM_RETURNED=18
PRIMER_RIGHT_NUM_RETURNED=18
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=18
PRIMER_PAIR_0_PENALTY=9.391639
PRIMER_LEFT_0_PENALTY=4.386829
PRIMER_RIGHT_0_PENALTY=5.004810
PRIMER_LEFT_0_SEQUENCE=AGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_0_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_0=925,23
PRIMER_RIGHT_0=1873,24
PRIMER_LEFT_0_TM=58.613
PRIMER_RIGHT_0_TM=58.995
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=949
PRIMER_PAIR_1_PENALTY=9.393527
PRIMER_LEFT_1_PENALTY=4.388717
PRIMER_RIGHT_1_PENALTY=5.004810
PRIMER_LEFT_1_SEQUENCE=GAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_1_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_1=926,22
PRIMER_RIGHT_1=1873,24
PRIMER_LEFT_1_TM=57.611
PRIMER_RIGHT_1_TM=58.995
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=948
PRIMER_PAIR_2_PENALTY=9.650190
PRIMER_LEFT_2_PENALTY=4.386829
PRIMER_RIGHT_2_PENALTY=5.263361
PRIMER_LEFT_2_SEQUENCE=AGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_2_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_2=925,23
PRIMER_RIGHT_2=1874,25
PRIMER_LEFT_2_TM=58.613
PRIMER_RIGHT_2_TM=59.737
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=950
PRIMER_PAIR_3_PENALTY=9.652078
PRIMER_LEFT_3_PENALTY=4.388717
PRIMER_RIGHT_3_PENALTY=5.263361
PRIMER_LEFT_3_SEQUENCE=GAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_3_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_3=926,22
PRIMER_RIGHT_3=1874,25
PRIMER_LEFT_3_TM=57.611
PRIMER_RIGHT_3_TM=59.737
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=949
PRIMER_PAIR_4_PENALTY=10.328456
PRIMER_LEFT_4_PENALTY=5.323646
PRIMER_RIGHT_4_PENALTY=5.004810
PRIMER_LEFT_4_SEQUENCE=AGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_4_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_4=927,21
PRIMER_RIGHT_4=1873,24
PRIMER_LEFT_4_TM=55.676
PRIMER_RIGHT_4_TM=58.995
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=947
PRIMER_PAIR_5_PENALTY=10.333705
PRIMER_LEFT_5_PENALTY=5.328895
PRIMER_RIGHT_5_PENALTY=5.004810
PRIMER_LEFT_5_SEQUENCE=TAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_5_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_5=924,24
PRIMER_RIGHT_5=1873,24
PRIMER_LEFT_5_TM=58.671
PRIMER_RIGHT_5_TM=58.995
PRIMER_LEFT_5_GC_PERCENT=41.667
PRIMER_RIGHT_5_GC_PERCENT=41.667
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=950
PRIMER_PAIR_6_PENALTY=10.449621
PRIMER_LEFT_6_PENALTY=5.444811
PRIMER_RIGHT_6_PENALTY=5.004810
PRIMER_LEFT_6_SEQUENCE=CTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_6_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_6=923,25
PRIMER_RIGHT_6=1873,24
PRIMER_LEFT_6_TM=59.555
PRIMER_RIGHT_6_TM=58.995
PRIMER_LEFT_6_GC_PERCENT=44.000
PRIMER_RIGHT_6_GC_PERCENT=41.667
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=951
PRIMER_PAIR_7_PENALTY=10.587007
PRIMER_LEFT_7_PENALTY=5.323646
PRIMER_RIGHT_7_PENALTY=5.263361
PRIMER_LEFT_7_SEQUENCE=AGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_7_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_7=927,21
PRIMER_RIGHT_7=1874,25
PRIMER_LEFT_7_TM=55.676
PRIMER_RIGHT_7_TM=59.737
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=948
PRIMER_PAIR_8_PENALTY=10.592256
PRIMER_LEFT_8_PENALTY=5.328895
PRIMER_RIGHT_8_PENALTY=5.263361
PRIMER_LEFT_8_SEQUENCE=TAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_8_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_8=924,24
PRIMER_RIGHT_8=1874,25
PRIMER_LEFT_8_TM=58.671
PRIMER_RIGHT_8_TM=59.737
PRIMER_LEFT_8_GC_PERCENT=41.667
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=951
PRIMER_PAIR_9_PENALTY=10.708172
PRIMER_LEFT_9_PENALTY=5.444811
PRIMER_RIGHT_9_PENALTY=5.263361
PRIMER_LEFT_9_SEQUENCE=CTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_9_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_9=923,25
PRIMER_RIGHT_9=1874,25
PRIMER_LEFT_9_TM=59.555
PRIMER_RIGHT_9_TM=59.737
PRIMER_LEFT_9_GC_PERCENT=44.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=952
PRIMER_PAIR_10_PENALTY=12.127204
PRIMER_LEFT_10_PENALTY=7.122394
PRIMER_RIGHT_10_PENALTY=5.004810
PRIMER_LEFT_10_SEQUENCE=TCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_10_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_10=922,26
PRIMER_RIGHT_10=1873,24
PRIMER_LEFT_10_TM=61.122
PRIMER_RIGHT_10_TM=58.995
PRIMER_LEFT_10_GC_PERCENT=42.308
PRIMER_RIGHT_10_GC_PERCENT=41.667
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=952
PRIMER_PAIR_11_PENALTY=12.385755
PRIMER_LEFT_11_PENALTY=7.122394
PRIMER_RIGHT_11_PENALTY=5.263361
PRIMER_LEFT_11_SEQUENCE=TCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_11_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_11=922,26
PRIMER_RIGHT_11=1874,25
PRIMER_LEFT_11_TM=61.122
PRIMER_RIGHT_11_TM=59.737
PRIMER_LEFT_11_GC_PERCENT=42.308
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=953
PRIMER_PAIR_12_PENALTY=13.300950
PRIMER_LEFT_12_PENALTY=8.296140
PRIMER_RIGHT_12_PENALTY=5.004810
PRIMER_LEFT_12_SEQUENCE=ATCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_12_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_12=921,27
PRIMER_RIGHT_12=1873,24
PRIMER_LEFT_12_TM=61.296
PRIMER_RIGHT_12_TM=58.995
PRIMER_LEFT_12_GC_PERCENT=40.741
PRIMER_RIGHT_12_GC_PERCENT=41.667
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=953
PRIMER_PAIR_13_PENALTY=13.559501
PRIMER_LEFT_13_PENALTY=8.296140
PRIMER_RIGHT_13_PENALTY=5.263361
PRIMER_LEFT_13_SEQUENCE=ATCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_13_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_13=921,27
PRIMER_RIGHT_13=1874,25
PRIMER_LEFT_13_TM=61.296
PRIMER_RIGHT_13_TM=59.737
PRIMER_LEFT_13_GC_PERCENT=40.741
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=954
PRIMER_PAIR_14_PENALTY=15.948054
PRIMER_LEFT_14_PENALTY=10.943244
PRIMER_RIGHT_14_PENALTY=5.004810
PRIMER_LEFT_14_SEQUENCE=CTATCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_14_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_14=919,29
PRIMER_RIGHT_14=1873,24
PRIMER_LEFT_14_TM=61.943
PRIMER_RIGHT_14_TM=58.995
PRIMER_LEFT_14_GC_PERCENT=41.379
PRIMER_RIGHT_14_GC_PERCENT=41.667
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=955
PRIMER_PAIR_15_PENALTY=16.206605
PRIMER_LEFT_15_PENALTY=10.943244
PRIMER_RIGHT_15_PENALTY=5.263361
PRIMER_LEFT_15_SEQUENCE=CTATCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_15_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_15=919,29
PRIMER_RIGHT_15=1874,25
PRIMER_LEFT_15_TM=61.943
PRIMER_RIGHT_15_TM=59.737
PRIMER_LEFT_15_GC_PERCENT=41.379
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=956
PRIMER_PAIR_16_PENALTY=17.496671
PRIMER_LEFT_16_PENALTY=12.491861
PRIMER_RIGHT_16_PENALTY=5.004810
PRIMER_LEFT_16_SEQUENCE=ACTATCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_16_SEQUENCE=CCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_16=918,30
PRIMER_RIGHT_16=1873,24
PRIMER_LEFT_16_TM=62.492
PRIMER_RIGHT_16_TM=58.995
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=956
PRIMER_PAIR_17_PENALTY=17.755222
PRIMER_LEFT_17_PENALTY=12.491861
PRIMER_RIGHT_17_PENALTY=5.263361
PRIMER_LEFT_17_SEQUENCE=ACTATCTAGAGGATATGTGAATCCCTTCTG
PRIMER_RIGHT_17_SEQUENCE=ACCATAAATCAACTTCTACACCGTC
PRIMER_LEFT_17=918,30
PRIMER_RIGHT_17=1874,25
PRIMER_LEFT_17_TM=62.492
PRIMER_RIGHT_17_TM=59.737
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=957
=
SEQUENCE_ID=MAGIC.329_4_8289822_8289910_ws-0_sf-2_1218488_3031_TRUE
SEQUENCE_TARGET=950,188
SEQUENCE_TEMPLATE=NCNNNNNNNNNNNTTTTTTNNNNNNNNNNNNATTNNNNNNACTCNACTANCNAATTGNNNAANNAGTCTACGGCCAAANNTGGCTACCTGCNTTGCTAAAGAAAGNATCAGCCAAAGAGTTGGCANTGCATGGAATANNCACAAGTAAAGTGAGAAGCATGNNNNNNNNNNNNNGNAANNTNTTCTACCTCTTNTAAGAANATTGAAAAATATNNNNNNNNNNNNNNNNNNNNNNANNNNAATGTTTAANNTAAAATGTAGTGATGNGCAACANANTAGGTAAAATAANAAANNNTTTNTTAAAAGTCATTGNNNNAANAAAGTTTNANGTCNATGGNNAAAAAANTAAGTTTNNAGTNTAANGCTTGATTNGNNNNNNNNTTCAATTTNAAGCTGTTGAANTATTTTNCNGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANCTATAANAAACNNGTGATGAACTTNGATTTCTGGTAGCCTAAAATCACNAGCTTCTTTATAAAGTTTCCNCTATGTCACATATTNNNGGTNNTTTATGTNNNNNNNNNNNNNNTCTCCGCNGAANCCCNGCCGCNACTNTCTCNCAACNNNNNNGACATTTAGACTTTANTANGANNNGACGACTTCAAAGTCTTTANTTATAACTNAATGACNTNNTAACTCNGGTCATTTTTTAATCTNTGTCTNNNNNNNNNNNCCTAATTATTTCNGACTAAAGAAANACTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCNNATCNTCATGACTCNACGTGGCTCGAGNCAANANTTTNANTATAAANNNANGAACTTNNTGTCTCAATCTCNTCAANCTTTNNCAAANCCTCTTGCANTACTNATCTTCTTCTTCNACTTGGTAAGTCTTTNTNTGNNCNNTCTTCATNCAATNGTTGNNTTTTTTGTNNNNNNNNNNNGAAGTTNGTAANTNAAANNTGTGTTTNCAAACNATTTNTGTGATNANTTTAGGTTTGCNATGGACAGTTCNGAAGCANAGCTAGATAGNATTGAGGCACANAGGTCAACNGATGNGTCTCATGATNAACNGANNNGAACANACNTACCNATGAACATACACGNTTCACAAGANTTNTCTCAAAGCGGANGAGATGGCNNNANGCCACNTACNGGAGACACCNAATCNGTGCNANANTNCCTNNCTAACCTNAGATTNTCGGATNTNTNCNCTNTNCNTNNTNAAGATGTCCNGATGANTCCNNNGNTNTTTAGNNAAATNAGTNATGGGAANAANGNATGTCTGGANNNGTTGAGAAGCCNNNNNNNNNNNNNNNNANGTNTCAAGAGCNANACAGGAGATTCNNTTCTTCANCTTGCTGNTANATGGGGTCATNTAGAACTAGTGAAGGAGATTGTCTNTGAATGNCNNNNNCTTCTNTTGGAGCNAAACTNNAGCGNTCAGACACCNCTNCATGTGGCGGCNNATNGCGGNCATACCNCTNTTNTTGAGNCTTTTGTNGCATTGGTAACATTTNCNTCAGCTANANNGNGTANNGAAGANAGTGAGAGAATGAANCCNTATGTTCTCAAGGACNANGATGGAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGTCTGGTGAANGCNAACNNGGATGCTCCTTTTNTTGNANNNNNNNNNNNNNTATCTNCCNNNNNNNNNNNNNNNNNNNCTGGAGNNGTATCNCTTGTNANNNNNNNNNTGNNNNNNNNNNNNNNNNAGGACCTTNAAGGGANGANNNNNNNNNNNNAGTCNAAGNTACAAGGNCNNAAATNTCTTGCACATGTTGCTTTNNTNNNNNNGAGTATAGGTTTNTTTTTCNCTTTAACCANNNNNNNNNNNNNNNNNNNNNNNCAANNATGTAAAACNAGTTTGAGANGGGANNNNCCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNATTNNTNGANNAGNTGTNCTTGATGNTATTCTTNANGAATNTCCAANTCNNNNNGANGANNNNNNNNNNNNNGGTNGGACTTGTCTTTCANTN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_8513545_8513567_ler-0_bur-0_1925162_116_TRUE
SEQUENCE_TARGET=950,122
SEQUENCE_TEMPLATE=TTTTTATTACTTTANTGTTTTTTATATGTCTATGGTGTGCTTATAGGTCTCTANATCTAGTTTTGTGTGTTATTTTCACGGCTATGATCTACAATTGGTTTTTATGTTCTTNCCTCTACCTGTGAGAGGAGAGTTAATATGTTATGGTNAAANGATATGATGCATAGCTGTGCAAAGANTAAAATTTAACTTCATATAAGGCTNCTTTTTGTGGCATATTGATGAAATATTAATNTTACACTTGGCTATTTTCATTGATCTGCTTAATGTTNCTGTTTATTGGTTTCATACGTGAATACAATCTAAACCAAATGATTGTGTAAATGTTTAGGTGGTGGACAACTACTACAGGCCGGACTTGAAGAAAGCTGCACTTGCTAGACTCAGCGCCATCAGCAAAGGTCTCAGGGTTGCCAAGTCNGGTGCCAAGCAAAGAAACAGACAAGCTTAAGCTTCTTCTTTCACAATCTGNTTTTTTTGAAGTAAAAGATTTTGTTGAACAGGTTTTATTTAAGTTGTTGCTTTGAAGTTGAACCATAANGCTATTCTCCTTTTTCNTTATCCTTATTATAAAGGAGTTAGAAGTTTATCTTNTTANNNNNNNNNNNNNNNNNNTGCTTGTGTTACATTTTGTTANATCNTTTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAACNAAGTCCTTATATNTNATGANNTTTGNAAGCCTANANNNNNNNNNNNNNNNNNNNNNNNNNNTTTANNTTNNTTGCATCANATGTGATGTTCTTGTGGACTNTAATGTTGACNTTNTCNTTNGATCCCATCAAAAACNAACTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACNGAAAGGTTTATATTAGGGTTTCNTAGACNTGNNTTAATTNTGTNCTCTNGCTGTNGCCGTCTCCAACNCCATTTCGAAGNNNNGNGAGANCGANGCNNNANNGAGNAAGNAAANNGNAGAAAGGAANNGGNNGNNANNANGGAGNNCNNNANCANNNNNNACANNNNNCTCCANAGNNNGTANNNNNTAAANCNTNAANNNTNNNNANCTTATNTNCANTNTCTCGTTTCCGNTTTNNTTAAGTTCGTTCTNGGNNTAGNNNNTAANNATTAGACTNAAATTGCAACTTNTNTTGATTNNAATTAGGTTTCTTGATGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANTCTNTAGATGAGTAANGATCATAAANATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTNGNNNCCTTTTATTGNATAGAAAGGTTGGCTTGTTGCTTAGGTNCATTTTNNATTNGAGNTTTTTTCGTTGTCANNATTGATCNNGTGGTNNNNNNNNNNNNNNNNNNNNTNNTTTCTAAATTGAACAGCACCTTCAAGAAGAAGGCACCCAATGCCATTAAAGAGATCAGGAAGTTTGCATTGAAAGCAATGGGAACNAANGACGTTAGAGTNGATGTTAAACTCAACAAGCAGATATGGAGCAAAGGTATCCGAGGTCCCCCGNGGAGNATNAGAGTCCGTGTTGCCCGTAAGAGAAACGATGATGAAGATGCCAAGGAAGAGTTCTTCTCNCTCGTCACNGTCGCTGAGATTCCGGCTGAAGGATTATCTGGTTTGGGTACTAAGGTCATTGAAGAAGAGGAGTGAAAATTGGTGAATGTACACTCACACANCCTTATCTTTNGATGNAAAGACGAAANNAAAAGACTTTGCTTGTTTTGATCACTTACCTATTTTGGTCATTTATCATNTCTTATGTTTTAAGTTCTATAGACAACAGATTTGGTACTACTCATACGAGTTTGATCTTTTTGTGACGCANTTTACTTCGCAATCATCTTTCTGATTCTGTACCAGACCGGTTTAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_304085_304332_rsch-4_hi-0_8168_198311_TRUE
SEQUENCE_TARGET=950,347
SEQUENCE_TEMPLATE=TTAANTATTATTNNAGAATATAATTTGATCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAGAATATTCCAAATGAAGTTCCATAATATTCTTCGGGGNGTAGAATAGAGGTGTTTTCTTACAATTTANGACGTCTATACTAGTAGCTATGATTGAAACCAAGACAAATCCAACAATTGTGGTCCAAAACAATATTGTACATTGCTTAAGGAAAGCGTTGAAGATAATAAATCANAATTAACCAAAAAGAAAGAAAAATAATAAATCATTGTTATGGTCGTCTTATGGATCCTAGTTATATACGAATTGAGTTTTCATTCATAGTTGAGAACGAATTCGTTTGCTCGCGTGTGAGTTTAGATCGTGGTTGTTTTTGTTTGATCGTCTANTANTGTTAATTAAGATCATTATTTCTTAAACCCTTGAATGGTTCTTCTAAAACCTATGCAATGACCGTGAAAANGTTGATGATATTAGTTTTAACCAAAGAAATGTTATGCTAATAATNATATTGTGATGTTAAATTAATAAAGAAANGGTTATGCTTAGCNTGNTCCCAAGCGATAATTCGTGTGTCCTATCATTAGGTTTGGAAAAACTCTACCAATCATAGACGATTAGACCAGTATAGTTGATTATAGTTTTCAGAATTCAGATTTCAGACCAAAGATAGAGTTGTGGACGTAGGTTTCTTGATTTTTGTTTTGTTTCTGTTTATNTTTTTTTGTCGTTATACATGGATAAAAAAAATTCCAACCAATCGTTACCTTGAGATGTGTAGTTGGACTTTTATTGCCTCANCAATACACTTATANAAAAGGTTCAATTTTCTGTCTAATTATGGACATGTTTATTTCAAATGTATGGTAATCTTCCAACACTTATAATACCATAATGAAAAATACTTCTGTNTNTATATATATTGAACTGAGATTCAATTAGTGCCACCCATGTGACCTGATGGACTCTCCGTAGACAAATGTGGGGGCAATTTTGTTGGATCAACCAAACCCAACATTTATTTTATATCTGATTTGAAGATTACATTAGTAAATAAACAATGGTTAGAGTCAAGTTTATACAAATATNTGGGACCCAACTAATAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAGTCACTATCTTTCGACGTGTCTCACTTGCAACCTAATCCTTTTCTGGAATGTAGNTTTTTTTTTNTTTGTCAATTTTATTTCTTTCTCTCTTAATCCATAAANNTTTTNGGCATTTTNGTANTACTGTNAAANAACTGATTAGCTATGTCTTCTATATATCTANNNNNNNNNNNAAGAATCATAGTTCATACTCNTNNTNNTATTCTTAATTTTCTGAAACTATCGATAAAATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.872936
PRIMER_LEFT_0_PENALTY=0.194601
PRIMER_RIGHT_0_PENALTY=0.678335
PRIMER_LEFT_0_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_0_SEQUENCE=ACGGAGAGTCCATCAGGTCA
PRIMER_LEFT_0=787,20
PRIMER_RIGHT_0=1419,20
PRIMER_LEFT_0_TM=60.195
PRIMER_RIGHT_0_TM=60.678
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.3000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=633
PRIMER_PAIR_1_PENALTY=0.875309
PRIMER_LEFT_1_PENALTY=0.194601
PRIMER_RIGHT_1_PENALTY=0.680708
PRIMER_LEFT_1_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_1_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_1=787,20
PRIMER_RIGHT_1=1451,20
PRIMER_LEFT_1_TM=60.195
PRIMER_RIGHT_1_TM=60.681
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=665
PRIMER_PAIR_2_PENALTY=1.138429
PRIMER_LEFT_2_PENALTY=0.457721
PRIMER_RIGHT_2_PENALTY=0.680708
PRIMER_LEFT_2_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_2_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_2=888,20
PRIMER_RIGHT_2=1451,20
PRIMER_LEFT_2_TM=59.542
PRIMER_RIGHT_2_TM=60.681
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=564
PRIMER_PAIR_3_PENALTY=1.610116
PRIMER_LEFT_3_PENALTY=0.194601
PRIMER_RIGHT_3_PENALTY=1.415515
PRIMER_LEFT_3_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_3_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_3=787,20
PRIMER_RIGHT_3=1417,20
PRIMER_LEFT_3_TM=60.195
PRIMER_RIGHT_3_TM=58.584
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=631
PRIMER_PAIR_4_PENALTY=1.678873
PRIMER_LEFT_4_PENALTY=0.998165
PRIMER_RIGHT_4_PENALTY=0.680708
PRIMER_LEFT_4_SEQUENCE=ACCTATGCAATGACCGTGAA
PRIMER_RIGHT_4_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_4=887,20
PRIMER_RIGHT_4=1451,20
PRIMER_LEFT_4_TM=59.002
PRIMER_RIGHT_4_TM=60.681
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=565
PRIMER_PAIR_5_PENALTY=1.691386
PRIMER_LEFT_5_PENALTY=0.194601
PRIMER_RIGHT_5_PENALTY=1.496786
PRIMER_LEFT_5_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_5_SEQUENCE=AATTGCCCCCACATTTGTCT
PRIMER_LEFT_5=787,20
PRIMER_RIGHT_5=1439,20
PRIMER_LEFT_5_TM=60.195
PRIMER_RIGHT_5_TM=61.497
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.3000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=653
PRIMER_PAIR_6_PENALTY=1.805753
PRIMER_LEFT_6_PENALTY=1.125045
PRIMER_RIGHT_6_PENALTY=0.680708
PRIMER_LEFT_6_SEQUENCE=AAAACCTATGCAATGACCGTG
PRIMER_RIGHT_6_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_6=884,21
PRIMER_RIGHT_6=1451,20
PRIMER_LEFT_6_TM=59.875
PRIMER_RIGHT_6_TM=60.681
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=11.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=568
PRIMER_PAIR_7_PENALTY=1.856849
PRIMER_LEFT_7_PENALTY=0.194601
PRIMER_RIGHT_7_PENALTY=1.662249
PRIMER_LEFT_7_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_7_SEQUENCE=TACGGAGAGTCCATCAGGTCA
PRIMER_LEFT_7=787,20
PRIMER_RIGHT_7=1420,21
PRIMER_LEFT_7_TM=60.195
PRIMER_RIGHT_7_TM=60.662
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=634
PRIMER_PAIR_8_PENALTY=1.873236
PRIMER_LEFT_8_PENALTY=0.457721
PRIMER_RIGHT_8_PENALTY=1.415515
PRIMER_LEFT_8_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_8_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_8=888,20
PRIMER_RIGHT_8=1417,20
PRIMER_LEFT_8_TM=59.542
PRIMER_RIGHT_8_TM=58.584
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=530
PRIMER_PAIR_9_PENALTY=1.954507
PRIMER_LEFT_9_PENALTY=0.457721
PRIMER_RIGHT_9_PENALTY=1.496786
PRIMER_LEFT_9_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_9_SEQUENCE=AATTGCCCCCACATTTGTCT
PRIMER_LEFT_9=888,20
PRIMER_RIGHT_9=1439,20
PRIMER_LEFT_9_TM=59.542
PRIMER_RIGHT_9_TM=61.497
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=552
PRIMER_PAIR_10_PENALTY=2.060639
PRIMER_LEFT_10_PENALTY=1.379931
PRIMER_RIGHT_10_PENALTY=0.680708
PRIMER_LEFT_10_SEQUENCE=ACCTATGCAATGACCGTGAAA
PRIMER_RIGHT_10_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_10=887,21
PRIMER_RIGHT_10=1451,20
PRIMER_LEFT_10_TM=60.380
PRIMER_RIGHT_10_TM=60.681
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=11.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=565
PRIMER_PAIR_11_PENALTY=2.060639
PRIMER_LEFT_11_PENALTY=1.379931
PRIMER_RIGHT_11_PENALTY=0.680708
PRIMER_LEFT_11_SEQUENCE=AACCTATGCAATGACCGTGAA
PRIMER_RIGHT_11_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_11=886,21
PRIMER_RIGHT_11=1451,20
PRIMER_LEFT_11_TM=60.380
PRIMER_RIGHT_11_TM=60.681
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=11.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=566
PRIMER_PAIR_12_PENALTY=2.091747
PRIMER_LEFT_12_PENALTY=1.413412
PRIMER_RIGHT_12_PENALTY=0.678335
PRIMER_LEFT_12_SEQUENCE=GTTTGCTCGCGTGTGAGTTT
PRIMER_RIGHT_12_SEQUENCE=ACGGAGAGTCCATCAGGTCA
PRIMER_LEFT_12=785,20
PRIMER_RIGHT_12=1419,20
PRIMER_LEFT_12_TM=61.413
PRIMER_RIGHT_12_TM=60.678
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=635
PRIMER_PAIR_13_PENALTY=2.094120
PRIMER_LEFT_13_PENALTY=1.413412
PRIMER_RIGHT_13_PENALTY=0.680708
PRIMER_LEFT_13_SEQUENCE=GTTTGCTCGCGTGTGAGTTT
PRIMER_RIGHT_13_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_13=785,20
PRIMER_RIGHT_13=1451,20
PRIMER_LEFT_13_TM=61.413
PRIMER_RIGHT_13_TM=60.681
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=11.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=667
PRIMER_PAIR_14_PENALTY=2.178371
PRIMER_LEFT_14_PENALTY=1.497663
PRIMER_RIGHT_14_PENALTY=0.680708
PRIMER_LEFT_14_SEQUENCE=AAACCTATGCAATGACCGTG
PRIMER_RIGHT_14_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_14=885,20
PRIMER_RIGHT_14=1451,20
PRIMER_LEFT_14_TM=58.502
PRIMER_RIGHT_14_TM=60.681
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=11.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=567
PRIMER_PAIR_15_PENALTY=2.279895
PRIMER_LEFT_15_PENALTY=0.194601
PRIMER_RIGHT_15_PENALTY=2.085294
PRIMER_LEFT_15_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_15_SEQUENCE=GTTGATCCAACAAAATTGCC
PRIMER_LEFT_15=787,20
PRIMER_RIGHT_15=1452,20
PRIMER_LEFT_15_TM=60.195
PRIMER_RIGHT_15_TM=57.915
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.3000
PRIMER_RIGHT_15_END_STABILITY=10.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=666
PRIMER_PAIR_16_PENALTY=2.382696
PRIMER_LEFT_16_PENALTY=1.704360
PRIMER_RIGHT_16_PENALTY=0.678335
PRIMER_LEFT_16_SEQUENCE=GCGTGTGAGTTTAGATCGTGG
PRIMER_RIGHT_16_SEQUENCE=ACGGAGAGTCCATCAGGTCA
PRIMER_LEFT_16=793,21
PRIMER_RIGHT_16=1419,20
PRIMER_LEFT_16_TM=60.704
PRIMER_RIGHT_16_TM=60.678
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=627
PRIMER_PAIR_17_PENALTY=2.384186
PRIMER_LEFT_17_PENALTY=0.194601
PRIMER_RIGHT_17_PENALTY=2.189585
PRIMER_LEFT_17_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_17_SEQUENCE=TCTACGGAGAGTCCATCAGG
PRIMER_LEFT_17=787,20
PRIMER_RIGHT_17=1422,20
PRIMER_LEFT_17_TM=60.195
PRIMER_RIGHT_17_TM=57.810
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.3000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=636
PRIMER_PAIR_18_PENALTY=2.385068
PRIMER_LEFT_18_PENALTY=1.704360
PRIMER_RIGHT_18_PENALTY=0.680708
PRIMER_LEFT_18_SEQUENCE=GCGTGTGAGTTTAGATCGTGG
PRIMER_RIGHT_18_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_18=793,21
PRIMER_RIGHT_18=1451,20
PRIMER_LEFT_18_TM=60.704
PRIMER_RIGHT_18_TM=60.681
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=11.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=659
PRIMER_PAIR_19_PENALTY=2.413680
PRIMER_LEFT_19_PENALTY=0.998165
PRIMER_RIGHT_19_PENALTY=1.415515
PRIMER_LEFT_19_SEQUENCE=ACCTATGCAATGACCGTGAA
PRIMER_RIGHT_19_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_19=887,20
PRIMER_RIGHT_19=1417,20
PRIMER_LEFT_19_TM=59.002
PRIMER_RIGHT_19_TM=58.584
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=531
PRIMER_PAIR_20_PENALTY=2.438471
PRIMER_LEFT_20_PENALTY=0.194601
PRIMER_RIGHT_20_PENALTY=2.243870
PRIMER_LEFT_20_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_20_SEQUENCE=CTACGGAGAGTCCATCAGGTC
PRIMER_LEFT_20=787,20
PRIMER_RIGHT_20=1421,21
PRIMER_LEFT_20_TM=60.195
PRIMER_RIGHT_20_TM=58.756
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=57.143
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.3000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=635
PRIMER_PAIR_21_PENALTY=2.438471
PRIMER_LEFT_21_PENALTY=0.194601
PRIMER_RIGHT_21_PENALTY=2.243870
PRIMER_LEFT_21_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_21_SEQUENCE=TCTACGGAGAGTCCATCAGGT
PRIMER_LEFT_21=787,20
PRIMER_RIGHT_21=1422,21
PRIMER_LEFT_21_TM=60.195
PRIMER_RIGHT_21_TM=58.756
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.3000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=636
PRIMER_PAIR_22_PENALTY=2.438471
PRIMER_LEFT_22_PENALTY=0.194601
PRIMER_RIGHT_22_PENALTY=2.243870
PRIMER_LEFT_22_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_22_SEQUENCE=GTCTACGGAGAGTCCATCAGG
PRIMER_LEFT_22=787,20
PRIMER_RIGHT_22=1423,21
PRIMER_LEFT_22_TM=60.195
PRIMER_RIGHT_22_TM=58.756
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=57.143
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.3000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=637
PRIMER_PAIR_23_PENALTY=2.460316
PRIMER_LEFT_23_PENALTY=0.194601
PRIMER_RIGHT_23_PENALTY=2.265715
PRIMER_LEFT_23_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_23_SEQUENCE=TTTGTCTACGGAGAGTCCATCA
PRIMER_LEFT_23=787,20
PRIMER_RIGHT_23=1426,22
PRIMER_LEFT_23_TM=60.195
PRIMER_RIGHT_23_TM=59.734
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.3000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=640
PRIMER_PAIR_24_PENALTY=2.494950
PRIMER_LEFT_24_PENALTY=0.998165
PRIMER_RIGHT_24_PENALTY=1.496786
PRIMER_LEFT_24_SEQUENCE=ACCTATGCAATGACCGTGAA
PRIMER_RIGHT_24_SEQUENCE=AATTGCCCCCACATTTGTCT
PRIMER_LEFT_24=887,20
PRIMER_RIGHT_24=1439,20
PRIMER_LEFT_24_TM=59.002
PRIMER_RIGHT_24_TM=61.497
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=553
PRIMER_PAIR_25_PENALTY=2.540560
PRIMER_LEFT_25_PENALTY=1.125045
PRIMER_RIGHT_25_PENALTY=1.415515
PRIMER_LEFT_25_SEQUENCE=AAAACCTATGCAATGACCGTG
PRIMER_RIGHT_25_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_25=884,21
PRIMER_RIGHT_25=1417,20
PRIMER_LEFT_25_TM=59.875
PRIMER_RIGHT_25_TM=58.584
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=9.9000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=534
PRIMER_PAIR_26_PENALTY=2.543015
PRIMER_LEFT_26_PENALTY=0.457721
PRIMER_RIGHT_26_PENALTY=2.085294
PRIMER_LEFT_26_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_26_SEQUENCE=GTTGATCCAACAAAATTGCC
PRIMER_LEFT_26=888,20
PRIMER_RIGHT_26=1452,20
PRIMER_LEFT_26_TM=59.542
PRIMER_RIGHT_26_TM=57.915
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=10.0000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=565
PRIMER_PAIR_27_PENALTY=2.548096
PRIMER_LEFT_27_PENALTY=0.194601
PRIMER_RIGHT_27_PENALTY=2.353495
PRIMER_LEFT_27_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_27_SEQUENCE=TACGGAGAGTCCATCAGGTC
PRIMER_LEFT_27=787,20
PRIMER_RIGHT_27=1420,20
PRIMER_LEFT_27_TM=60.195
PRIMER_RIGHT_27_TM=57.647
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.3000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=634
PRIMER_PAIR_28_PENALTY=2.549297
PRIMER_LEFT_28_PENALTY=1.870961
PRIMER_RIGHT_28_PENALTY=0.678335
PRIMER_LEFT_28_SEQUENCE=CCTATGCAATGACCGTGAAAA
PRIMER_RIGHT_28_SEQUENCE=ACGGAGAGTCCATCAGGTCA
PRIMER_LEFT_28=888,21
PRIMER_RIGHT_28=1419,20
PRIMER_LEFT_28_TM=60.871
PRIMER_RIGHT_28_TM=60.678
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=532
PRIMER_PAIR_29_PENALTY=2.551669
PRIMER_LEFT_29_PENALTY=1.870961
PRIMER_RIGHT_29_PENALTY=0.680708
PRIMER_LEFT_29_SEQUENCE=CCTATGCAATGACCGTGAAAA
PRIMER_RIGHT_29_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_29=888,21
PRIMER_RIGHT_29=1451,20
PRIMER_LEFT_29_TM=60.871
PRIMER_RIGHT_29_TM=60.681
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.3000
PRIMER_RIGHT_29_END_STABILITY=11.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=564
PRIMER_PAIR_30_PENALTY=2.566944
PRIMER_LEFT_30_PENALTY=0.194601
PRIMER_RIGHT_30_PENALTY=2.372343
PRIMER_LEFT_30_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_30_SEQUENCE=GTCTACGGAGAGTCCATCAGGT
PRIMER_LEFT_30=787,20
PRIMER_RIGHT_30=1423,22
PRIMER_LEFT_30_TM=60.195
PRIMER_RIGHT_30_TM=59.628
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=54.545
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.3000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=637
PRIMER_PAIR_31_PENALTY=2.621830
PRIMER_LEFT_31_PENALTY=1.125045
PRIMER_RIGHT_31_PENALTY=1.496786
PRIMER_LEFT_31_SEQUENCE=AAAACCTATGCAATGACCGTG
PRIMER_RIGHT_31_SEQUENCE=AATTGCCCCCACATTTGTCT
PRIMER_LEFT_31=884,21
PRIMER_RIGHT_31=1439,20
PRIMER_LEFT_31_TM=59.875
PRIMER_RIGHT_31_TM=61.497
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.9000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=556
PRIMER_PAIR_32_PENALTY=2.641134
PRIMER_LEFT_32_PENALTY=0.194601
PRIMER_RIGHT_32_PENALTY=2.446533
PRIMER_LEFT_32_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_32_SEQUENCE=GTTGATCCAACAAAATTGCCC
PRIMER_LEFT_32=787,20
PRIMER_RIGHT_32=1452,21
PRIMER_LEFT_32_TM=60.195
PRIMER_RIGHT_32_TM=61.447
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.3000
PRIMER_RIGHT_32_END_STABILITY=11.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=666
PRIMER_PAIR_33_PENALTY=2.641134
PRIMER_LEFT_33_PENALTY=0.194601
PRIMER_RIGHT_33_PENALTY=2.446533
PRIMER_LEFT_33_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_33_SEQUENCE=GGTTGATCCAACAAAATTGCC
PRIMER_LEFT_33=787,20
PRIMER_RIGHT_33=1453,21
PRIMER_LEFT_33_TM=60.195
PRIMER_RIGHT_33_TM=61.447
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.3000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=667
PRIMER_PAIR_34_PENALTY=2.701591
PRIMER_LEFT_34_PENALTY=0.457721
PRIMER_RIGHT_34_PENALTY=2.243870
PRIMER_LEFT_34_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_34_SEQUENCE=CTACGGAGAGTCCATCAGGTC
PRIMER_LEFT_34=888,20
PRIMER_RIGHT_34=1421,21
PRIMER_LEFT_34_TM=59.542
PRIMER_RIGHT_34_TM=58.756
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=57.143
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.3000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=534
PRIMER_PAIR_35_PENALTY=2.791101
PRIMER_LEFT_35_PENALTY=2.110393
PRIMER_RIGHT_35_PENALTY=0.680708
PRIMER_LEFT_35_SEQUENCE=TAAAACCTATGCAATGACCGTG
PRIMER_RIGHT_35_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_35=883,22
PRIMER_RIGHT_35=1451,20
PRIMER_LEFT_35_TM=59.890
PRIMER_RIGHT_35_TM=60.681
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.9000
PRIMER_RIGHT_35_END_STABILITY=11.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=569
PRIMER_PAIR_36_PENALTY=2.795446
PRIMER_LEFT_36_PENALTY=1.379931
PRIMER_RIGHT_36_PENALTY=1.415515
PRIMER_LEFT_36_SEQUENCE=ACCTATGCAATGACCGTGAAA
PRIMER_RIGHT_36_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_36=887,21
PRIMER_RIGHT_36=1417,20
PRIMER_LEFT_36_TM=60.380
PRIMER_RIGHT_36_TM=58.584
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=531
PRIMER_PAIR_37_PENALTY=2.795446
PRIMER_LEFT_37_PENALTY=1.379931
PRIMER_RIGHT_37_PENALTY=1.415515
PRIMER_LEFT_37_SEQUENCE=AACCTATGCAATGACCGTGAA
PRIMER_RIGHT_37_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_37=886,21
PRIMER_RIGHT_37=1417,20
PRIMER_LEFT_37_TM=60.380
PRIMER_RIGHT_37_TM=58.584
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=532
PRIMER_PAIR_38_PENALTY=2.811216
PRIMER_LEFT_38_PENALTY=0.457721
PRIMER_RIGHT_38_PENALTY=2.353495
PRIMER_LEFT_38_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_38_SEQUENCE=TACGGAGAGTCCATCAGGTC
PRIMER_LEFT_38=888,20
PRIMER_RIGHT_38=1420,20
PRIMER_LEFT_38_TM=59.542
PRIMER_RIGHT_38_TM=57.647
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.3000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=533
PRIMER_PAIR_39_PENALTY=2.828927
PRIMER_LEFT_39_PENALTY=1.413412
PRIMER_RIGHT_39_PENALTY=1.415515
PRIMER_LEFT_39_SEQUENCE=GTTTGCTCGCGTGTGAGTTT
PRIMER_RIGHT_39_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_39=785,20
PRIMER_RIGHT_39=1417,20
PRIMER_LEFT_39_TM=61.413
PRIMER_RIGHT_39_TM=58.584
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=633
PRIMER_PAIR_40_PENALTY=2.843676
PRIMER_LEFT_40_PENALTY=0.194601
PRIMER_RIGHT_40_PENALTY=2.649076
PRIMER_LEFT_40_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_40_SEQUENCE=TCTACGGAGAGTCCATCAGGTC
PRIMER_LEFT_40=787,20
PRIMER_RIGHT_40=1422,22
PRIMER_LEFT_40_TM=60.195
PRIMER_RIGHT_40_TM=60.649
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=54.545
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.3000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=636
PRIMER_PAIR_41_PENALTY=2.876716
PRIMER_LEFT_41_PENALTY=1.379931
PRIMER_RIGHT_41_PENALTY=1.496786
PRIMER_LEFT_41_SEQUENCE=ACCTATGCAATGACCGTGAAA
PRIMER_RIGHT_41_SEQUENCE=AATTGCCCCCACATTTGTCT
PRIMER_LEFT_41=887,21
PRIMER_RIGHT_41=1439,20
PRIMER_LEFT_41_TM=60.380
PRIMER_RIGHT_41_TM=61.497
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=553
PRIMER_PAIR_42_PENALTY=2.876716
PRIMER_LEFT_42_PENALTY=1.379931
PRIMER_RIGHT_42_PENALTY=1.496786
PRIMER_LEFT_42_SEQUENCE=AACCTATGCAATGACCGTGAA
PRIMER_RIGHT_42_SEQUENCE=AATTGCCCCCACATTTGTCT
PRIMER_LEFT_42=886,21
PRIMER_RIGHT_42=1439,20
PRIMER_LEFT_42_TM=60.380
PRIMER_RIGHT_42_TM=61.497
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=554
PRIMER_PAIR_43_PENALTY=2.893915
PRIMER_LEFT_43_PENALTY=0.194601
PRIMER_RIGHT_43_PENALTY=2.699314
PRIMER_LEFT_43_SEQUENCE=TTGCTCGCGTGTGAGTTTAG
PRIMER_RIGHT_43_SEQUENCE=TTGTCTACGGAGAGTCCATCA
PRIMER_LEFT_43=787,20
PRIMER_RIGHT_43=1425,21
PRIMER_LEFT_43_TM=60.195
PRIMER_RIGHT_43_TM=58.301
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.3000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=639
PRIMER_PAIR_44_PENALTY=2.901862
PRIMER_LEFT_44_PENALTY=2.223527
PRIMER_RIGHT_44_PENALTY=0.678335
PRIMER_LEFT_44_SEQUENCE=GCTTAAGGAAAGCGTTGAAGA
PRIMER_RIGHT_44_SEQUENCE=ACGGAGAGTCCATCAGGTCA
PRIMER_LEFT_44=650,21
PRIMER_RIGHT_44=1419,20
PRIMER_LEFT_44_TM=58.776
PRIMER_RIGHT_44_TM=60.678
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=770
PRIMER_PAIR_45_PENALTY=2.904235
PRIMER_LEFT_45_PENALTY=2.223527
PRIMER_RIGHT_45_PENALTY=0.680708
PRIMER_LEFT_45_SEQUENCE=GCTTAAGGAAAGCGTTGAAGA
PRIMER_RIGHT_45_SEQUENCE=TTGATCCAACAAAATTGCCC
PRIMER_LEFT_45=650,21
PRIMER_RIGHT_45=1451,20
PRIMER_LEFT_45_TM=58.776
PRIMER_RIGHT_45_TM=60.681
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=11.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=802
PRIMER_PAIR_46_PENALTY=2.904254
PRIMER_LEFT_46_PENALTY=0.457721
PRIMER_RIGHT_46_PENALTY=2.446533
PRIMER_LEFT_46_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_46_SEQUENCE=GTTGATCCAACAAAATTGCCC
PRIMER_LEFT_46=888,20
PRIMER_RIGHT_46=1452,21
PRIMER_LEFT_46_TM=59.542
PRIMER_RIGHT_46_TM=61.447
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=11.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=565
PRIMER_PAIR_47_PENALTY=2.904254
PRIMER_LEFT_47_PENALTY=0.457721
PRIMER_RIGHT_47_PENALTY=2.446533
PRIMER_LEFT_47_SEQUENCE=CCTATGCAATGACCGTGAAA
PRIMER_RIGHT_47_SEQUENCE=GGTTGATCCAACAAAATTGCC
PRIMER_LEFT_47=888,20
PRIMER_RIGHT_47=1453,21
PRIMER_LEFT_47_TM=59.542
PRIMER_RIGHT_47_TM=61.447
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.3000
PRIMER_RIGHT_47_END_STABILITY=10.0000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=566
PRIMER_PAIR_48_PENALTY=2.910197
PRIMER_LEFT_48_PENALTY=1.413412
PRIMER_RIGHT_48_PENALTY=1.496786
PRIMER_LEFT_48_SEQUENCE=GTTTGCTCGCGTGTGAGTTT
PRIMER_RIGHT_48_SEQUENCE=AATTGCCCCCACATTTGTCT
PRIMER_LEFT_48=785,20
PRIMER_RIGHT_48=1439,20
PRIMER_LEFT_48_TM=61.413
PRIMER_RIGHT_48_TM=61.497
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=655
PRIMER_PAIR_49_PENALTY=2.913178
PRIMER_LEFT_49_PENALTY=1.497663
PRIMER_RIGHT_49_PENALTY=1.415515
PRIMER_LEFT_49_SEQUENCE=AAACCTATGCAATGACCGTG
PRIMER_RIGHT_49_SEQUENCE=GGAGAGTCCATCAGGTCACA
PRIMER_LEFT_49=885,20
PRIMER_RIGHT_49=1417,20
PRIMER_LEFT_49_TM=58.502
PRIMER_RIGHT_49_TM=58.584
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=533
=
SEQUENCE_ID=MAGIC.433_5_13992199_13992379_tsu-0_sf-2_179581_102395_TRUE
SEQUENCE_TARGET=950,280
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTTATNACATCCAAGTTGGATGGAGTTTGAATTTTTTGAAAATGACAAGAAAATTTTGTTCACGAATTTAATCTTAAAATAAAANNGCTCAAATTGAACATTANTTGGAATGNAAAACACAAGATACAAACTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_799645_800035_ct-1_mt-0_673170_75615_TRUE
SEQUENCE_TARGET=950,490
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGATCTCTGATTAAACTATTTNGATAGATTTNACCTACCTCACTCNATCATATTTTNTTTTCACACNANATTNANGGAAAAATCAATAAGTATCACGCTATATATTACCACAATAATTAACCTNCCATGATTAAACTACTAACTTATCATTAAAGTTTGTAATCGAGCGAATCTCTGAATTAATTAGTGTANGTCTGACAAAAAGAAAANNTTAATGTAACAAACCGNGAGGTGCCGGTAGCTCAACAAATGNTACAGGTTTCGTGAGACTAATTCGGCTCGGCTTAATGGCTGATTCGTGTGTATTGCTGTAGTGGGNTTGGTAATGCACCTAGCCANTCTTTATTTTAGGAGAACAATGTGTTTTTGTTNAAGGCATGATAAACGTTACTNATTTNNTTTTNTTTGTAANNAAAAATCAAATGAAGACGACATTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTGAATGCAAGTCCGTAGTTGGTTTAAAGTTAAAGATTAAGTGACTTTATCANNNNNNNNNNAAAAANATTATGTTGNTGATTAAATCGTGTACTANAATTGTTGCAAACACATTTATTTTCCTACTTAGATTTAGCGCTGGATAATCAACTATCCGACTAATTAANTATGTAAGTGAGTATGCATCTTGATAAACAGTGCTTCACAGATTTTGTGGTTTGCGAACATGAATTTTATGGAAGTTGAGCCGTTGGAAGATAAATGCTTCATTTGTTTTTATGGATAACTCACAACTTGCTTTGTGGATAATATAATTAATGAATTGATTTACCAGTCAACTTTAAGTGTTAGTGAATAATTNTTTAAGNCCAAAANNNNNNNAAAATTCTNGGTCGTGTGAGGAAACTGCGATGAGANNNAGGTAGTTGAAANAATTGTNTTTGNATGCATGTGAAANTCATGCAAAACAAATACANGTACATATAAGTTTTGACCNGTNTTNAACTTCCNNNTTTTTTTTTTTTTTACCAGTCTTGAACTATCTAATATCTANNNNNNNNTTACGACATTNGTTAGTTTCATGAAATGGCGTCTCCGATCCGATAACTTTAAATAAGTAATTAAGAACGTTGAATNTCACGACTACTATTCCATATGCAAATCTTCAAGTGTCTTATATTTNGTAAATAAAATTAAGAATTTGTATAATTAGGTNNNGTTNGAATATAATAATCATCACAATTGAACCGACACCTCAATTAAGAGCGACCATATAGTATGGAAGCACGATACAGTCGACCGTTAGTGAANCTTANAATAATATCATCAATGANTGTTTCTTGGGTGTATAAAATTANTGAAANGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.621890
PRIMER_LEFT_0_PENALTY=0.605838
PRIMER_RIGHT_0_PENALTY=0.016052
PRIMER_LEFT_0_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_0_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_0=869,20
PRIMER_RIGHT_0=1727,20
PRIMER_LEFT_0_TM=60.606
PRIMER_RIGHT_0_TM=59.984
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=859
PRIMER_PAIR_1_PENALTY=0.768338
PRIMER_LEFT_1_PENALTY=0.605838
PRIMER_RIGHT_1_PENALTY=0.162500
PRIMER_LEFT_1_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_1_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_1=869,20
PRIMER_RIGHT_1=1726,20
PRIMER_LEFT_1_TM=60.606
PRIMER_RIGHT_1_TM=59.837
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=858
PRIMER_PAIR_2_PENALTY=0.980928
PRIMER_LEFT_2_PENALTY=0.605838
PRIMER_RIGHT_2_PENALTY=0.375090
PRIMER_LEFT_2_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_2_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_2=869,20
PRIMER_RIGHT_2=1570,20
PRIMER_LEFT_2_TM=60.606
PRIMER_RIGHT_2_TM=60.375
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=702
PRIMER_PAIR_3_PENALTY=1.141065
PRIMER_LEFT_3_PENALTY=0.605838
PRIMER_RIGHT_3_PENALTY=0.535226
PRIMER_LEFT_3_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_3_SEQUENCE=CATGTTCGCAAACCACAAAA
PRIMER_LEFT_3=869,20
PRIMER_RIGHT_3=1541,20
PRIMER_LEFT_3_TM=60.606
PRIMER_RIGHT_3_TM=60.535
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=673
PRIMER_PAIR_4_PENALTY=1.170515
PRIMER_LEFT_4_PENALTY=1.154463
PRIMER_RIGHT_4_PENALTY=0.016052
PRIMER_LEFT_4_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_4_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_4=877,21
PRIMER_RIGHT_4=1727,20
PRIMER_LEFT_4_TM=60.154
PRIMER_RIGHT_4_TM=59.984
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=851
PRIMER_PAIR_5_PENALTY=1.316963
PRIMER_LEFT_5_PENALTY=1.154463
PRIMER_RIGHT_5_PENALTY=0.162500
PRIMER_LEFT_5_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_5_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_5=877,21
PRIMER_RIGHT_5=1726,20
PRIMER_LEFT_5_TM=60.154
PRIMER_RIGHT_5_TM=59.837
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=850
PRIMER_PAIR_6_PENALTY=1.420177
PRIMER_LEFT_6_PENALTY=0.605838
PRIMER_RIGHT_6_PENALTY=0.814339
PRIMER_LEFT_6_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_6_SEQUENCE=GCATTTATCTTCCAACGGCT
PRIMER_LEFT_6=869,20
PRIMER_RIGHT_6=1577,20
PRIMER_LEFT_6_TM=60.606
PRIMER_RIGHT_6_TM=59.186
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=11.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=709
PRIMER_PAIR_7_PENALTY=1.529553
PRIMER_LEFT_7_PENALTY=1.154463
PRIMER_RIGHT_7_PENALTY=0.375090
PRIMER_LEFT_7_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_7_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_7=877,21
PRIMER_RIGHT_7=1570,20
PRIMER_LEFT_7_TM=60.154
PRIMER_RIGHT_7_TM=60.375
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=694
PRIMER_PAIR_8_PENALTY=1.645901
PRIMER_LEFT_8_PENALTY=0.605838
PRIMER_RIGHT_8_PENALTY=1.040063
PRIMER_LEFT_8_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_8_SEQUENCE=TCTCATCGCAGTTTCCTCAC
PRIMER_LEFT_8=869,20
PRIMER_RIGHT_8=1728,20
PRIMER_LEFT_8_TM=60.606
PRIMER_RIGHT_8_TM=58.960
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=860
PRIMER_PAIR_9_PENALTY=1.654925
PRIMER_LEFT_9_PENALTY=1.638872
PRIMER_RIGHT_9_PENALTY=0.016052
PRIMER_LEFT_9_SEQUENCE=GCTGATTCGTGTGTATTGCTG
PRIMER_RIGHT_9_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_9=878,21
PRIMER_RIGHT_9=1727,20
PRIMER_LEFT_9_TM=59.361
PRIMER_RIGHT_9_TM=59.984
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=850
PRIMER_PAIR_10_PENALTY=1.687237
PRIMER_LEFT_10_PENALTY=0.605838
PRIMER_RIGHT_10_PENALTY=1.081398
PRIMER_LEFT_10_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_10_SEQUENCE=CATTTATCTTCCAACGGCTCA
PRIMER_LEFT_10=869,20
PRIMER_RIGHT_10=1576,21
PRIMER_LEFT_10_TM=60.606
PRIMER_RIGHT_10_TM=60.081
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=708
PRIMER_PAIR_11_PENALTY=1.714881
PRIMER_LEFT_11_PENALTY=0.605838
PRIMER_RIGHT_11_PENALTY=1.109043
PRIMER_LEFT_11_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_11_SEQUENCE=AGCATTTATCTTCCAACGGCT
PRIMER_LEFT_11=869,20
PRIMER_RIGHT_11=1578,21
PRIMER_LEFT_11_TM=60.606
PRIMER_RIGHT_11_TM=60.109
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=710
PRIMER_PAIR_12_PENALTY=1.722589
PRIMER_LEFT_12_PENALTY=0.605838
PRIMER_RIGHT_12_PENALTY=1.116751
PRIMER_LEFT_12_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_12_SEQUENCE=CAACGGCTCAACTTCCATAAA
PRIMER_LEFT_12=869,20
PRIMER_RIGHT_12=1565,21
PRIMER_LEFT_12_TM=60.606
PRIMER_RIGHT_12_TM=60.117
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=6.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=697
PRIMER_PAIR_13_PENALTY=1.801373
PRIMER_LEFT_13_PENALTY=1.638872
PRIMER_RIGHT_13_PENALTY=0.162500
PRIMER_LEFT_13_SEQUENCE=GCTGATTCGTGTGTATTGCTG
PRIMER_RIGHT_13_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_13=878,21
PRIMER_RIGHT_13=1726,20
PRIMER_LEFT_13_TM=59.361
PRIMER_RIGHT_13_TM=59.837
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=849
PRIMER_PAIR_14_PENALTY=1.835011
PRIMER_LEFT_14_PENALTY=0.605838
PRIMER_RIGHT_14_PENALTY=1.229173
PRIMER_LEFT_14_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_14_SEQUENCE=CAACGGCTCAACTTCCATAA
PRIMER_LEFT_14=869,20
PRIMER_RIGHT_14=1565,20
PRIMER_LEFT_14_TM=60.606
PRIMER_RIGHT_14_TM=58.771
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=6.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=697
PRIMER_PAIR_15_PENALTY=1.882768
PRIMER_LEFT_15_PENALTY=1.866716
PRIMER_RIGHT_15_PENALTY=0.016052
PRIMER_LEFT_15_SEQUENCE=CGTGTGTATTGCTGTAGTGGG
PRIMER_RIGHT_15_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_15=885,21
PRIMER_RIGHT_15=1727,20
PRIMER_LEFT_15_TM=59.133
PRIMER_RIGHT_15_TM=59.984
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=843
PRIMER_PAIR_16_PENALTY=1.887491
PRIMER_LEFT_16_PENALTY=1.871438
PRIMER_RIGHT_16_PENALTY=0.016052
PRIMER_LEFT_16_SEQUENCE=TGGCTGATTCGTGTGTATTG
PRIMER_RIGHT_16_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_16=876,20
PRIMER_RIGHT_16=1727,20
PRIMER_LEFT_16_TM=58.129
PRIMER_RIGHT_16_TM=59.984
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.2000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=852
PRIMER_PAIR_17_PENALTY=1.968802
PRIMER_LEFT_17_PENALTY=1.154463
PRIMER_RIGHT_17_PENALTY=0.814339
PRIMER_LEFT_17_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_17_SEQUENCE=GCATTTATCTTCCAACGGCT
PRIMER_LEFT_17=877,21
PRIMER_RIGHT_17=1577,20
PRIMER_LEFT_17_TM=60.154
PRIMER_RIGHT_17_TM=59.186
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=11.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=701
PRIMER_PAIR_18_PENALTY=2.013962
PRIMER_LEFT_18_PENALTY=1.638872
PRIMER_RIGHT_18_PENALTY=0.375090
PRIMER_LEFT_18_SEQUENCE=GCTGATTCGTGTGTATTGCTG
PRIMER_RIGHT_18_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_18=878,21
PRIMER_RIGHT_18=1570,20
PRIMER_LEFT_18_TM=59.361
PRIMER_RIGHT_18_TM=60.375
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=693
PRIMER_PAIR_19_PENALTY=2.027380
PRIMER_LEFT_19_PENALTY=1.652290
PRIMER_RIGHT_19_PENALTY=0.375090
PRIMER_LEFT_19_SEQUENCE=GCTTAATGGCTGATTCGTGTG
PRIMER_RIGHT_19_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_19=870,21
PRIMER_RIGHT_19=1570,20
PRIMER_LEFT_19_TM=60.652
PRIMER_RIGHT_19_TM=60.375
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=701
PRIMER_PAIR_20_PENALTY=2.029216
PRIMER_LEFT_20_PENALTY=1.866716
PRIMER_RIGHT_20_PENALTY=0.162500
PRIMER_LEFT_20_SEQUENCE=CGTGTGTATTGCTGTAGTGGG
PRIMER_RIGHT_20_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_20=885,21
PRIMER_RIGHT_20=1726,20
PRIMER_LEFT_20_TM=59.133
PRIMER_RIGHT_20_TM=59.837
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=842
PRIMER_PAIR_21_PENALTY=2.033939
PRIMER_LEFT_21_PENALTY=1.871438
PRIMER_RIGHT_21_PENALTY=0.162500
PRIMER_LEFT_21_SEQUENCE=TGGCTGATTCGTGTGTATTG
PRIMER_RIGHT_21_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_21=876,20
PRIMER_RIGHT_21=1726,20
PRIMER_LEFT_21_TM=58.129
PRIMER_RIGHT_21_TM=59.837
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.2000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=851
PRIMER_PAIR_22_PENALTY=2.126994
PRIMER_LEFT_22_PENALTY=2.110942
PRIMER_RIGHT_22_PENALTY=0.016052
PRIMER_LEFT_22_SEQUENCE=AATGGCTGATTCGTGTGTATTG
PRIMER_RIGHT_22_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_22=874,22
PRIMER_RIGHT_22=1727,20
PRIMER_LEFT_22_TM=59.889
PRIMER_RIGHT_22_TM=59.984
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=854
PRIMER_PAIR_23_PENALTY=2.168248
PRIMER_LEFT_23_PENALTY=2.152196
PRIMER_RIGHT_23_PENALTY=0.016052
PRIMER_LEFT_23_SEQUENCE=GCTTAATGGCTGATTCGTGT
PRIMER_RIGHT_23_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_23=870,20
PRIMER_RIGHT_23=1727,20
PRIMER_LEFT_23_TM=57.848
PRIMER_RIGHT_23_TM=59.984
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=858
PRIMER_PAIR_24_PENALTY=2.187516
PRIMER_LEFT_24_PENALTY=1.652290
PRIMER_RIGHT_24_PENALTY=0.535226
PRIMER_LEFT_24_SEQUENCE=GCTTAATGGCTGATTCGTGTG
PRIMER_RIGHT_24_SEQUENCE=CATGTTCGCAAACCACAAAA
PRIMER_LEFT_24=870,21
PRIMER_RIGHT_24=1541,20
PRIMER_LEFT_24_TM=60.652
PRIMER_RIGHT_24_TM=60.535
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=672
PRIMER_PAIR_25_PENALTY=2.194526
PRIMER_LEFT_25_PENALTY=1.154463
PRIMER_RIGHT_25_PENALTY=1.040063
PRIMER_LEFT_25_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_25_SEQUENCE=TCTCATCGCAGTTTCCTCAC
PRIMER_LEFT_25=877,21
PRIMER_RIGHT_25=1728,20
PRIMER_LEFT_25_TM=60.154
PRIMER_RIGHT_25_TM=58.960
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=852
PRIMER_PAIR_26_PENALTY=2.220958
PRIMER_LEFT_26_PENALTY=2.204906
PRIMER_RIGHT_26_PENALTY=0.016052
PRIMER_LEFT_26_SEQUENCE=GCTGATTCGTGTGTATTGCTGT
PRIMER_RIGHT_26_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_26=878,22
PRIMER_RIGHT_26=1727,20
PRIMER_LEFT_26_TM=60.205
PRIMER_RIGHT_26_TM=59.984
PRIMER_LEFT_26_GC_PERCENT=45.455
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=850
PRIMER_PAIR_27_PENALTY=2.230352
PRIMER_LEFT_27_PENALTY=0.605838
PRIMER_RIGHT_27_PENALTY=1.624514
PRIMER_LEFT_27_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_27_SEQUENCE=ATCTTCCAACGGCTCAACTTC
PRIMER_LEFT_27=869,20
PRIMER_RIGHT_27=1571,21
PRIMER_LEFT_27_TM=60.606
PRIMER_RIGHT_27_TM=60.625
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=703
PRIMER_PAIR_28_PENALTY=2.235861
PRIMER_LEFT_28_PENALTY=1.154463
PRIMER_RIGHT_28_PENALTY=1.081398
PRIMER_LEFT_28_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_28_SEQUENCE=CATTTATCTTCCAACGGCTCA
PRIMER_LEFT_28=877,21
PRIMER_RIGHT_28=1576,21
PRIMER_LEFT_28_TM=60.154
PRIMER_RIGHT_28_TM=60.081
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=700
PRIMER_PAIR_29_PENALTY=2.241806
PRIMER_LEFT_29_PENALTY=1.866716
PRIMER_RIGHT_29_PENALTY=0.375090
PRIMER_LEFT_29_SEQUENCE=CGTGTGTATTGCTGTAGTGGG
PRIMER_RIGHT_29_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_29=885,21
PRIMER_RIGHT_29=1570,20
PRIMER_LEFT_29_TM=59.133
PRIMER_RIGHT_29_TM=60.375
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=686
PRIMER_PAIR_30_PENALTY=2.245036
PRIMER_LEFT_30_PENALTY=0.605838
PRIMER_RIGHT_30_PENALTY=1.639198
PRIMER_LEFT_30_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_30_SEQUENCE=TTTATCTTCCAACGGCTCAA
PRIMER_LEFT_30=869,20
PRIMER_RIGHT_30=1574,20
PRIMER_LEFT_30_TM=60.606
PRIMER_RIGHT_30_TM=58.361
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.4000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=706
PRIMER_PAIR_31_PENALTY=2.246528
PRIMER_LEFT_31_PENALTY=1.871438
PRIMER_RIGHT_31_PENALTY=0.375090
PRIMER_LEFT_31_SEQUENCE=TGGCTGATTCGTGTGTATTG
PRIMER_RIGHT_31_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_31=876,20
PRIMER_RIGHT_31=1570,20
PRIMER_LEFT_31_TM=58.129
PRIMER_RIGHT_31_TM=60.375
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.2000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=695
PRIMER_PAIR_32_PENALTY=2.263505
PRIMER_LEFT_32_PENALTY=1.154463
PRIMER_RIGHT_32_PENALTY=1.109043
PRIMER_LEFT_32_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_32_SEQUENCE=AGCATTTATCTTCCAACGGCT
PRIMER_LEFT_32=877,21
PRIMER_RIGHT_32=1578,21
PRIMER_LEFT_32_TM=60.154
PRIMER_RIGHT_32_TM=60.109
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=11.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=702
PRIMER_PAIR_33_PENALTY=2.271214
PRIMER_LEFT_33_PENALTY=1.154463
PRIMER_RIGHT_33_PENALTY=1.116751
PRIMER_LEFT_33_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_33_SEQUENCE=CAACGGCTCAACTTCCATAAA
PRIMER_LEFT_33=877,21
PRIMER_RIGHT_33=1565,21
PRIMER_LEFT_33_TM=60.154
PRIMER_RIGHT_33_TM=60.117
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=689
PRIMER_PAIR_34_PENALTY=2.273442
PRIMER_LEFT_34_PENALTY=2.110942
PRIMER_RIGHT_34_PENALTY=0.162500
PRIMER_LEFT_34_SEQUENCE=AATGGCTGATTCGTGTGTATTG
PRIMER_RIGHT_34_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_34=874,22
PRIMER_RIGHT_34=1726,20
PRIMER_LEFT_34_TM=59.889
PRIMER_RIGHT_34_TM=59.837
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.2000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=853
PRIMER_PAIR_35_PENALTY=2.300328
PRIMER_LEFT_35_PENALTY=1.866716
PRIMER_RIGHT_35_PENALTY=0.433612
PRIMER_LEFT_35_SEQUENCE=CGTGTGTATTGCTGTAGTGGG
PRIMER_RIGHT_35_SEQUENCE=TGTTCGCAAACCACAAAATC
PRIMER_LEFT_35=885,21
PRIMER_RIGHT_35=1539,20
PRIMER_LEFT_35_TM=59.133
PRIMER_RIGHT_35_TM=59.566
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=655
PRIMER_PAIR_36_PENALTY=2.367406
PRIMER_LEFT_36_PENALTY=2.204906
PRIMER_RIGHT_36_PENALTY=0.162500
PRIMER_LEFT_36_SEQUENCE=GCTGATTCGTGTGTATTGCTGT
PRIMER_RIGHT_36_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_36=878,22
PRIMER_RIGHT_36=1726,20
PRIMER_LEFT_36_TM=60.205
PRIMER_RIGHT_36_TM=59.837
PRIMER_LEFT_36_GC_PERCENT=45.455
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=849
PRIMER_PAIR_37_PENALTY=2.381755
PRIMER_LEFT_37_PENALTY=2.365703
PRIMER_RIGHT_37_PENALTY=0.016052
PRIMER_LEFT_37_SEQUENCE=CGGCTTAATGGCTGATTCGT
PRIMER_RIGHT_37_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_37=868,20
PRIMER_RIGHT_37=1727,20
PRIMER_LEFT_37_TM=62.366
PRIMER_RIGHT_37_TM=59.984
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=860
PRIMER_PAIR_38_PENALTY=2.383636
PRIMER_LEFT_38_PENALTY=1.154463
PRIMER_RIGHT_38_PENALTY=1.229173
PRIMER_LEFT_38_SEQUENCE=GGCTGATTCGTGTGTATTGCT
PRIMER_RIGHT_38_SEQUENCE=CAACGGCTCAACTTCCATAA
PRIMER_LEFT_38=877,21
PRIMER_RIGHT_38=1565,20
PRIMER_LEFT_38_TM=60.154
PRIMER_RIGHT_38_TM=58.771
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=6.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=689
PRIMER_PAIR_39_PENALTY=2.388034
PRIMER_LEFT_39_PENALTY=2.371982
PRIMER_RIGHT_39_PENALTY=0.016052
PRIMER_LEFT_39_SEQUENCE=GGCTTAATGGCTGATTCGTGT
PRIMER_RIGHT_39_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_39=869,21
PRIMER_RIGHT_39=1727,20
PRIMER_LEFT_39_TM=61.372
PRIMER_RIGHT_39_TM=59.984
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.1000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=859
PRIMER_PAIR_40_PENALTY=2.390737
PRIMER_LEFT_40_PENALTY=0.605838
PRIMER_RIGHT_40_PENALTY=1.784899
PRIMER_LEFT_40_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_40_SEQUENCE=TTTATCTTCCAACGGCTCAAC
PRIMER_LEFT_40=869,20
PRIMER_RIGHT_40=1574,21
PRIMER_LEFT_40_TM=60.606
PRIMER_RIGHT_40_TM=59.215
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.4000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=706
PRIMER_PAIR_41_PENALTY=2.401943
PRIMER_LEFT_41_PENALTY=1.866716
PRIMER_RIGHT_41_PENALTY=0.535226
PRIMER_LEFT_41_SEQUENCE=CGTGTGTATTGCTGTAGTGGG
PRIMER_RIGHT_41_SEQUENCE=CATGTTCGCAAACCACAAAA
PRIMER_LEFT_41=885,21
PRIMER_RIGHT_41=1541,20
PRIMER_LEFT_41_TM=59.133
PRIMER_RIGHT_41_TM=60.535
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=657
PRIMER_PAIR_42_PENALTY=2.453212
PRIMER_LEFT_42_PENALTY=1.638872
PRIMER_RIGHT_42_PENALTY=0.814339
PRIMER_LEFT_42_SEQUENCE=GCTGATTCGTGTGTATTGCTG
PRIMER_RIGHT_42_SEQUENCE=GCATTTATCTTCCAACGGCT
PRIMER_LEFT_42=878,21
PRIMER_RIGHT_42=1577,20
PRIMER_LEFT_42_TM=59.361
PRIMER_RIGHT_42_TM=59.186
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=11.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=700
PRIMER_PAIR_43_PENALTY=2.454727
PRIMER_LEFT_43_PENALTY=0.605838
PRIMER_RIGHT_43_PENALTY=1.848889
PRIMER_LEFT_43_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_43_SEQUENCE=CTCATCGCAGTTTCCTCACAC
PRIMER_LEFT_43=869,20
PRIMER_RIGHT_43=1727,21
PRIMER_LEFT_43_TM=60.606
PRIMER_RIGHT_43_TM=60.849
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=859
PRIMER_PAIR_44_PENALTY=2.466629
PRIMER_LEFT_44_PENALTY=1.652290
PRIMER_RIGHT_44_PENALTY=0.814339
PRIMER_LEFT_44_SEQUENCE=GCTTAATGGCTGATTCGTGTG
PRIMER_RIGHT_44_SEQUENCE=GCATTTATCTTCCAACGGCT
PRIMER_LEFT_44=870,21
PRIMER_RIGHT_44=1577,20
PRIMER_LEFT_44_TM=60.652
PRIMER_RIGHT_44_TM=59.186
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=11.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=708
PRIMER_PAIR_45_PENALTY=2.486032
PRIMER_LEFT_45_PENALTY=2.110942
PRIMER_RIGHT_45_PENALTY=0.375090
PRIMER_LEFT_45_SEQUENCE=AATGGCTGATTCGTGTGTATTG
PRIMER_RIGHT_45_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_45=874,22
PRIMER_RIGHT_45=1570,20
PRIMER_LEFT_45_TM=59.889
PRIMER_RIGHT_45_TM=60.375
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.2000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=697
PRIMER_PAIR_46_PENALTY=2.499783
PRIMER_LEFT_46_PENALTY=2.483731
PRIMER_RIGHT_46_PENALTY=0.016052
PRIMER_LEFT_46_SEQUENCE=ATGGCTGATTCGTGTGTATTG
PRIMER_RIGHT_46_SEQUENCE=CTCATCGCAGTTTCCTCACA
PRIMER_LEFT_46=875,21
PRIMER_RIGHT_46=1727,20
PRIMER_LEFT_46_TM=58.516
PRIMER_RIGHT_46_TM=59.984
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.2000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=853
PRIMER_PAIR_47_PENALTY=2.527286
PRIMER_LEFT_47_PENALTY=2.152196
PRIMER_RIGHT_47_PENALTY=0.375090
PRIMER_LEFT_47_SEQUENCE=GCTTAATGGCTGATTCGTGT
PRIMER_RIGHT_47_SEQUENCE=TCTTCCAACGGCTCAACTTC
PRIMER_LEFT_47=870,20
PRIMER_RIGHT_47=1570,20
PRIMER_LEFT_47_TM=57.848
PRIMER_RIGHT_47_TM=60.375
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=701
PRIMER_PAIR_48_PENALTY=2.528204
PRIMER_LEFT_48_PENALTY=2.365703
PRIMER_RIGHT_48_PENALTY=0.162500
PRIMER_LEFT_48_SEQUENCE=CGGCTTAATGGCTGATTCGT
PRIMER_RIGHT_48_SEQUENCE=TCATCGCAGTTTCCTCACAC
PRIMER_LEFT_48=868,20
PRIMER_RIGHT_48=1726,20
PRIMER_LEFT_48_TM=62.366
PRIMER_RIGHT_48_TM=59.837
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=859
PRIMER_PAIR_49_PENALTY=2.550499
PRIMER_LEFT_49_PENALTY=0.605838
PRIMER_RIGHT_49_PENALTY=1.944661
PRIMER_LEFT_49_SEQUENCE=GGCTTAATGGCTGATTCGTG
PRIMER_RIGHT_49_SEQUENCE=GCATTTATCTTCCAACGGCTC
PRIMER_LEFT_49=869,20
PRIMER_RIGHT_49=1577,21
PRIMER_LEFT_49_TM=60.606
PRIMER_RIGHT_49_TM=60.945
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.4000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=709
=
SEQUENCE_ID=MAGIC.105_4_1909303_1909844_wu-0_sf-2_233689_22135_TRUE
SEQUENCE_TARGET=950,641
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNTAATATNATTATCTATANNNANAAGAACTNATAATTAACTGANNAAAANAAAANATTGTTGTNTTNTACTNTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_12945003_12945487_can-0_wu-0_62919_7376_TRUE
SEQUENCE_TARGET=950,584
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17456063_17456544_bur-0_po-0_64227_25729_TRUE
SEQUENCE_TARGET=950,581
SEQUENCE_TEMPLATE=GGTGGATGAGTTGATAGATAANCTTAAAAATGAAGCTCATGTNGTTTGAAGGAAGAAACNNNTGNNNNNNNNNNNNNNNNNNNNNNNCTTCTNNNTNCTTATCTACGTTGCTGTTTCAGGCATNANGTNGAAGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTTCTTATTAANAANATAGTTNATGTNCTCNNGANCNAGAANANAGNAGNAANACACACATANNTNTTNTTAACTAAGCCANACAAGTNNTTTCTTCATATCCNGAANTTAGNCAANTGCGATTTTCCATCNAAGATGNNTGCNANGTATCCTACAGTGTANTCTGGTATNGTGATGTCTNTGTACTTTGCAGGGTTTTCATCAGAAAGAAGCTCTTTTATCGGTCCATAAACNGNGGAATTNGGNAACACTCCCGANCTAACGAAGCAAGCAACTGAAATNCGCGGTCCNTCTCTGTTTGCCNGNACCCTATGCTCCATACTTAAGAACTTATCGTTTGTGATNAGCTGCAAAACAAAATGACTCAAGTGTATTGNTAANGAAAGACTCTGTTTTGAGTTAAAAGGANGCATCAATCGAGCTTGCCTGCATGAAATCACCGATNCTGATGACAAGAGCTCCAGGAACAGGAGNTACATCAACCCAAGAATCTTGATGAAGAACTNGAAGACCACCNATTTGNTCTTGAAGAAGAATNGTTATGAANGAATTGTCGGTGTGTTTACTTATGCCCAAAGTTAGGTCAGGTTGTGGACAAGGTGGATANTAATGGCAGAGCATAAGCAAACCCTTGAGACAATCCATGTTCNTAAGAATCTCANAGTCTAAACCTAAAGCTTCTGAGAGAAGTTGGAAGAGTAAACCTCCTAATNCCATTACATGCTTCGAGTATTCAATCACAACATCCCTAAGATNNTANCATCGTNTANTCAGAANCATCAAAACANTAATAAAGCGAAATNAANATTGCNAAACNTNTTTACCTGCAAGCTAGTGGGATTTCCTCTGGCTTTAGAGGATCTTGNGGACAAGTGTAGCAAGTGAAAGAGTCTTTCCAATTCATAGGAGAAGAATNATGAATATCNAAGTTGGTGTTNTAAGCAAATCTTNTATTGAAATCTGTAGCAAAATACTGTTTCTTGACCTCTGGATCTTCCTCATGAAATCTTCGAACACCTTGTTTGATATCTTNAAGANCAGTTAAAGGAATACCATGATTGATNACTTGGAAAAATCCCCAATTCTCAGCTGCTTCTTTAATCTTNGAAACGAGGACGTTTNTTGCAGCAGATGTGTTACCATCTNCGNGGTCAATGATNGGGACAGTGAGGTTTAAGCCANAGATANCAGAAGGTTNGTTGTTAGATAAANTTGNAGAAGAAGGGATATGGAAGATTCGAGGAACTTCGGTTATTTTGGTATCNACNAGTCCTTTCACACCTNCTTTTGTNNNNACAAAAGCNTTGAGTTCGTTCAAACGANCGAGTTNTNCAGATTTTTCTGTCNNNATGTTTTNTTTTTTTCTGTCNNNNTACGTTGAGANANGAAGTAANGACAGAAGCGNAGTCGTGAAGATANGAATTNTTGAAGTTANTNGTGGANCTGTGTGACCCATTTTGTAGANATTTTGTTCGTTGACTGTGACTTGAGTGAGGTATCATCGANAGCGACTTGTGCAANGAAGAANAAGTTTCAAACTTCAAAGGTAGNAATCANNNTTTGCAACCAATNCCATCTCNTTCGTGGGCTAGGGTTCTNCTNTANTGGGCTTGNGCNGTTTTTAGATTTTCCNGTCNNAAAATTTCAGNTTTTATTTNTGCCCATGTATTNNNNNNNGTTGGGACTATGGCCTTTTGTTTGTATAACCNAAAGAACAAAATACTGCTACAAATATGAAAATAANTACAATATTTCCATGCGNGATTTTGNATTTNATGTGANAACATTCTTGCNAGCCGATTTNGCTAGCNTTTGGANNNNNNNNNNNNNCTACTTCTGACTNTTANGNCTCAAAAANTAAGCTTTTGTAGTTTNAAAAAGATAACNTATAGTTTGACTTGTTAGCTACAAGCCTAGAATGCTGTTTTAANCATGANGTAGAAAACANTTTTTCTTNNTGGTACAATGGTTGAGGAACTCTANAGCGGGNACANANAAAANGTANCGTAACANATGNAAAACGNACAAATATATATNTTGCACTNGGACAAGTNTTTTCTTCATATCATGTAATTAGNCAAATACGACTTTTCATCAAANATGNNCGCAANAAATCCTGCAGTGTACTCTGGTATGGTGATATCTTTGTACTTAGCNGGGTTNTCATCAGACAAAAGATCTTTAATCGGTCCATAAACCGTNGAATTTGGAGAAAGACTCGAGCTAAAGAAGCACGCAACTGAAATCCGCGGTCTATCTTTGTTCGGTCGTACCCTATGCTCC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.955665
PRIMER_LEFT_0_PENALTY=0.468413
PRIMER_RIGHT_0_PENALTY=2.487253
PRIMER_LEFT_0_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_0_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_0=821,20
PRIMER_RIGHT_0=1691,22
PRIMER_LEFT_0_TM=59.532
PRIMER_RIGHT_0_TM=59.513
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=871
PRIMER_PAIR_1_PENALTY=3.006674
PRIMER_LEFT_1_PENALTY=0.519421
PRIMER_RIGHT_1_PENALTY=2.487253
PRIMER_LEFT_1_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_1_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_1=810,20
PRIMER_RIGHT_1=1691,22
PRIMER_LEFT_1_TM=59.481
PRIMER_RIGHT_1_TM=59.513
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=882
PRIMER_PAIR_2_PENALTY=3.083517
PRIMER_LEFT_2_PENALTY=0.468413
PRIMER_RIGHT_2_PENALTY=2.615104
PRIMER_LEFT_2_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_2_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_2=821,20
PRIMER_RIGHT_2=1687,22
PRIMER_LEFT_2_TM=59.532
PRIMER_RIGHT_2_TM=59.385
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=867
PRIMER_PAIR_3_PENALTY=3.134526
PRIMER_LEFT_3_PENALTY=0.519421
PRIMER_RIGHT_3_PENALTY=2.615104
PRIMER_LEFT_3_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_3_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_3=810,20
PRIMER_RIGHT_3=1687,22
PRIMER_LEFT_3_TM=59.481
PRIMER_RIGHT_3_TM=59.385
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=878
PRIMER_PAIR_4_PENALTY=3.486207
PRIMER_LEFT_4_PENALTY=0.468413
PRIMER_RIGHT_4_PENALTY=3.017794
PRIMER_LEFT_4_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_4_SEQUENCE=CTCAAGTCACAGTCAACGAACA
PRIMER_LEFT_4=821,20
PRIMER_RIGHT_4=1688,22
PRIMER_LEFT_4_TM=59.532
PRIMER_RIGHT_4_TM=58.982
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=868
PRIMER_PAIR_5_PENALTY=3.486207
PRIMER_LEFT_5_PENALTY=0.468413
PRIMER_RIGHT_5_PENALTY=3.017794
PRIMER_LEFT_5_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_5_SEQUENCE=CACTCAAGTCACAGTCAACGAA
PRIMER_LEFT_5=821,20
PRIMER_RIGHT_5=1690,22
PRIMER_LEFT_5_TM=59.532
PRIMER_RIGHT_5_TM=58.982
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=8.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=870
PRIMER_PAIR_6_PENALTY=3.537216
PRIMER_LEFT_6_PENALTY=0.519421
PRIMER_RIGHT_6_PENALTY=3.017794
PRIMER_LEFT_6_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_6_SEQUENCE=CTCAAGTCACAGTCAACGAACA
PRIMER_LEFT_6=810,20
PRIMER_RIGHT_6=1688,22
PRIMER_LEFT_6_TM=59.481
PRIMER_RIGHT_6_TM=58.982
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.1000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=879
PRIMER_PAIR_7_PENALTY=3.537216
PRIMER_LEFT_7_PENALTY=0.519421
PRIMER_RIGHT_7_PENALTY=3.017794
PRIMER_LEFT_7_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_7_SEQUENCE=CACTCAAGTCACAGTCAACGAA
PRIMER_LEFT_7=810,20
PRIMER_RIGHT_7=1690,22
PRIMER_LEFT_7_TM=59.481
PRIMER_RIGHT_7_TM=58.982
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.1000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=881
PRIMER_PAIR_8_PENALTY=3.593933
PRIMER_LEFT_8_PENALTY=0.468413
PRIMER_RIGHT_8_PENALTY=3.125520
PRIMER_LEFT_8_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_8_SEQUENCE=CAAGTCACAGTCAACGAACAAA
PRIMER_LEFT_8=821,20
PRIMER_RIGHT_8=1686,22
PRIMER_LEFT_8_TM=59.532
PRIMER_RIGHT_8_TM=58.874
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=866
PRIMER_PAIR_9_PENALTY=3.604354
PRIMER_LEFT_9_PENALTY=0.468413
PRIMER_RIGHT_9_PENALTY=3.135941
PRIMER_LEFT_9_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_9_SEQUENCE=ACTCAAGTCACAGTCAACGAACA
PRIMER_LEFT_9=821,20
PRIMER_RIGHT_9=1689,23
PRIMER_LEFT_9_TM=59.532
PRIMER_RIGHT_9_TM=59.864
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=869
PRIMER_PAIR_10_PENALTY=3.604354
PRIMER_LEFT_10_PENALTY=0.468413
PRIMER_RIGHT_10_PENALTY=3.135941
PRIMER_LEFT_10_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_10_SEQUENCE=CACTCAAGTCACAGTCAACGAAC
PRIMER_LEFT_10=821,20
PRIMER_RIGHT_10=1690,23
PRIMER_LEFT_10_TM=59.532
PRIMER_RIGHT_10_TM=59.864
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=870
PRIMER_PAIR_11_PENALTY=3.610958
PRIMER_LEFT_11_PENALTY=0.468413
PRIMER_RIGHT_11_PENALTY=3.142546
PRIMER_LEFT_11_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_11_SEQUENCE=TCAAGTCACAGTCAACGAACA
PRIMER_LEFT_11=821,20
PRIMER_RIGHT_11=1687,21
PRIMER_LEFT_11_TM=59.532
PRIMER_RIGHT_11_TM=57.857
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=867
PRIMER_PAIR_12_PENALTY=3.644942
PRIMER_LEFT_12_PENALTY=0.519421
PRIMER_RIGHT_12_PENALTY=3.125520
PRIMER_LEFT_12_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_12_SEQUENCE=CAAGTCACAGTCAACGAACAAA
PRIMER_LEFT_12=810,20
PRIMER_RIGHT_12=1686,22
PRIMER_LEFT_12_TM=59.481
PRIMER_RIGHT_12_TM=58.874
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.1000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=2.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=877
PRIMER_PAIR_13_PENALTY=3.655363
PRIMER_LEFT_13_PENALTY=0.519421
PRIMER_RIGHT_13_PENALTY=3.135941
PRIMER_LEFT_13_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_13_SEQUENCE=ACTCAAGTCACAGTCAACGAACA
PRIMER_LEFT_13=810,20
PRIMER_RIGHT_13=1689,23
PRIMER_LEFT_13_TM=59.481
PRIMER_RIGHT_13_TM=59.864
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.1000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=880
PRIMER_PAIR_14_PENALTY=3.655363
PRIMER_LEFT_14_PENALTY=0.519421
PRIMER_RIGHT_14_PENALTY=3.135941
PRIMER_LEFT_14_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_14_SEQUENCE=CACTCAAGTCACAGTCAACGAAC
PRIMER_LEFT_14=810,20
PRIMER_RIGHT_14=1690,23
PRIMER_LEFT_14_TM=59.481
PRIMER_RIGHT_14_TM=59.864
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=881
PRIMER_PAIR_15_PENALTY=3.661967
PRIMER_LEFT_15_PENALTY=0.519421
PRIMER_RIGHT_15_PENALTY=3.142546
PRIMER_LEFT_15_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_15_SEQUENCE=TCAAGTCACAGTCAACGAACA
PRIMER_LEFT_15=810,20
PRIMER_RIGHT_15=1687,21
PRIMER_LEFT_15_TM=59.481
PRIMER_RIGHT_15_TM=57.857
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.1000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=878
PRIMER_PAIR_16_PENALTY=3.740149
PRIMER_LEFT_16_PENALTY=1.125045
PRIMER_RIGHT_16_PENALTY=2.615104
PRIMER_LEFT_16_SEQUENCE=TCGGTGTGTTTACTTATGCCC
PRIMER_RIGHT_16_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_16=751,21
PRIMER_RIGHT_16=1687,22
PRIMER_LEFT_16_TM=59.875
PRIMER_RIGHT_16_TM=59.385
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=11.2000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=937
PRIMER_PAIR_17_PENALTY=3.791248
PRIMER_LEFT_17_PENALTY=1.303996
PRIMER_RIGHT_17_PENALTY=2.487253
PRIMER_LEFT_17_SEQUENCE=TGGCAGAGCATAAGCAAACC
PRIMER_RIGHT_17_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_17=808,20
PRIMER_RIGHT_17=1691,22
PRIMER_LEFT_17_TM=61.304
PRIMER_RIGHT_17_TM=59.513
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=884
PRIMER_PAIR_18_PENALTY=3.843942
PRIMER_LEFT_18_PENALTY=0.468413
PRIMER_RIGHT_18_PENALTY=3.375529
PRIMER_LEFT_18_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_18_SEQUENCE=CTCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_18=821,20
PRIMER_RIGHT_18=1688,23
PRIMER_LEFT_18_TM=59.532
PRIMER_RIGHT_18_TM=60.376
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=868
PRIMER_PAIR_19_PENALTY=3.894951
PRIMER_LEFT_19_PENALTY=0.519421
PRIMER_RIGHT_19_PENALTY=3.375529
PRIMER_LEFT_19_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_19_SEQUENCE=CTCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_19=810,20
PRIMER_RIGHT_19=1688,23
PRIMER_LEFT_19_TM=59.481
PRIMER_RIGHT_19_TM=60.376
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.1000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=879
PRIMER_PAIR_20_PENALTY=3.919100
PRIMER_LEFT_20_PENALTY=1.303996
PRIMER_RIGHT_20_PENALTY=2.615104
PRIMER_LEFT_20_SEQUENCE=TGGCAGAGCATAAGCAAACC
PRIMER_RIGHT_20_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_20=808,20
PRIMER_RIGHT_20=1687,22
PRIMER_LEFT_20_TM=61.304
PRIMER_RIGHT_20_TM=59.385
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=880
PRIMER_PAIR_21_PENALTY=3.946015
PRIMER_LEFT_21_PENALTY=1.458762
PRIMER_RIGHT_21_PENALTY=2.487253
PRIMER_LEFT_21_SEQUENCE=CAAACCCTTGAGACAATCCA
PRIMER_RIGHT_21_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_21=822,20
PRIMER_RIGHT_21=1691,22
PRIMER_LEFT_21_TM=58.541
PRIMER_RIGHT_21_TM=59.513
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=870
PRIMER_PAIR_22_PENALTY=3.953837
PRIMER_LEFT_22_PENALTY=1.466585
PRIMER_RIGHT_22_PENALTY=2.487253
PRIMER_LEFT_22_SEQUENCE=AATGGCAGAGCATAAGCAAA
PRIMER_RIGHT_22_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_22=806,20
PRIMER_RIGHT_22=1691,22
PRIMER_LEFT_22_TM=58.533
PRIMER_RIGHT_22_TM=59.513
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.8000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=886
PRIMER_PAIR_23_PENALTY=3.977566
PRIMER_LEFT_23_PENALTY=1.490313
PRIMER_RIGHT_23_PENALTY=2.487253
PRIMER_LEFT_23_SEQUENCE=AGCAAACCCTTGAGACAATCC
PRIMER_RIGHT_23_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_23=820,21
PRIMER_RIGHT_23=1691,22
PRIMER_LEFT_23_TM=60.490
PRIMER_RIGHT_23_TM=59.513
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=872
PRIMER_PAIR_24_PENALTY=3.997502
PRIMER_LEFT_24_PENALTY=0.468413
PRIMER_RIGHT_24_PENALTY=3.529090
PRIMER_LEFT_24_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_24_SEQUENCE=CTCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_24=821,20
PRIMER_RIGHT_24=1692,23
PRIMER_LEFT_24_TM=59.532
PRIMER_RIGHT_24_TM=60.529
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=47.826
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=872
PRIMER_PAIR_25_PENALTY=4.048511
PRIMER_LEFT_25_PENALTY=0.519421
PRIMER_RIGHT_25_PENALTY=3.529090
PRIMER_LEFT_25_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_25_SEQUENCE=CTCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_25=810,20
PRIMER_RIGHT_25=1692,23
PRIMER_LEFT_25_TM=59.481
PRIMER_RIGHT_25_TM=60.529
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.826
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=9.1000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=883
PRIMER_PAIR_26_PENALTY=4.050947
PRIMER_LEFT_26_PENALTY=0.468413
PRIMER_RIGHT_26_PENALTY=3.582535
PRIMER_LEFT_26_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_26_SEQUENCE=CACTCAAGTCACAGTCAACGA
PRIMER_LEFT_26=821,20
PRIMER_RIGHT_26=1690,21
PRIMER_LEFT_26_TM=59.532
PRIMER_RIGHT_26_TM=57.417
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.1000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=870
PRIMER_PAIR_27_PENALTY=4.073867
PRIMER_LEFT_27_PENALTY=1.458762
PRIMER_RIGHT_27_PENALTY=2.615104
PRIMER_LEFT_27_SEQUENCE=CAAACCCTTGAGACAATCCA
PRIMER_RIGHT_27_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_27=822,20
PRIMER_RIGHT_27=1687,22
PRIMER_LEFT_27_TM=58.541
PRIMER_RIGHT_27_TM=59.385
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=866
PRIMER_PAIR_28_PENALTY=4.081689
PRIMER_LEFT_28_PENALTY=1.466585
PRIMER_RIGHT_28_PENALTY=2.615104
PRIMER_LEFT_28_SEQUENCE=AATGGCAGAGCATAAGCAAA
PRIMER_RIGHT_28_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_28=806,20
PRIMER_RIGHT_28=1687,22
PRIMER_LEFT_28_TM=58.533
PRIMER_RIGHT_28_TM=59.385
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.8000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=2.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=882
PRIMER_PAIR_29_PENALTY=4.101956
PRIMER_LEFT_29_PENALTY=0.519421
PRIMER_RIGHT_29_PENALTY=3.582535
PRIMER_LEFT_29_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_29_SEQUENCE=CACTCAAGTCACAGTCAACGA
PRIMER_LEFT_29=810,20
PRIMER_RIGHT_29=1690,21
PRIMER_LEFT_29_TM=59.481
PRIMER_RIGHT_29_TM=57.417
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.1000
PRIMER_RIGHT_29_END_STABILITY=8.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=881
PRIMER_PAIR_30_PENALTY=4.105417
PRIMER_LEFT_30_PENALTY=1.490313
PRIMER_RIGHT_30_PENALTY=2.615104
PRIMER_LEFT_30_SEQUENCE=AGCAAACCCTTGAGACAATCC
PRIMER_RIGHT_30_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_30=820,21
PRIMER_RIGHT_30=1687,22
PRIMER_LEFT_30_TM=60.490
PRIMER_RIGHT_30_TM=59.385
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=868
PRIMER_PAIR_31_PENALTY=4.108275
PRIMER_LEFT_31_PENALTY=1.621022
PRIMER_RIGHT_31_PENALTY=2.487253
PRIMER_LEFT_31_SEQUENCE=AATGGCAGAGCATAAGCAAAC
PRIMER_RIGHT_31_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_31=806,21
PRIMER_RIGHT_31=1691,22
PRIMER_LEFT_31_TM=59.379
PRIMER_RIGHT_31_TM=59.513
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=886
PRIMER_PAIR_32_PENALTY=4.111995
PRIMER_LEFT_32_PENALTY=0.468413
PRIMER_RIGHT_32_PENALTY=3.643582
PRIMER_LEFT_32_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_32_SEQUENCE=CAAGTCACAGTCAACGAACAA
PRIMER_LEFT_32=821,20
PRIMER_RIGHT_32=1686,21
PRIMER_LEFT_32_TM=59.532
PRIMER_RIGHT_32_TM=57.356
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=866
PRIMER_PAIR_33_PENALTY=4.142839
PRIMER_LEFT_33_PENALTY=1.125045
PRIMER_RIGHT_33_PENALTY=3.017794
PRIMER_LEFT_33_SEQUENCE=TCGGTGTGTTTACTTATGCCC
PRIMER_RIGHT_33_SEQUENCE=CTCAAGTCACAGTCAACGAACA
PRIMER_LEFT_33=751,21
PRIMER_RIGHT_33=1688,22
PRIMER_LEFT_33_TM=59.875
PRIMER_RIGHT_33_TM=58.982
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=11.2000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=938
PRIMER_PAIR_34_PENALTY=4.142839
PRIMER_LEFT_34_PENALTY=1.125045
PRIMER_RIGHT_34_PENALTY=3.017794
PRIMER_LEFT_34_SEQUENCE=TCGGTGTGTTTACTTATGCCC
PRIMER_RIGHT_34_SEQUENCE=CACTCAAGTCACAGTCAACGAA
PRIMER_LEFT_34=751,21
PRIMER_RIGHT_34=1690,22
PRIMER_LEFT_34_TM=59.875
PRIMER_RIGHT_34_TM=58.982
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=11.2000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=940
PRIMER_PAIR_35_PENALTY=4.163004
PRIMER_LEFT_35_PENALTY=0.519421
PRIMER_RIGHT_35_PENALTY=3.643582
PRIMER_LEFT_35_SEQUENCE=GCAGAGCATAAGCAAACCCT
PRIMER_RIGHT_35_SEQUENCE=CAAGTCACAGTCAACGAACAA
PRIMER_LEFT_35=810,20
PRIMER_RIGHT_35=1686,21
PRIMER_LEFT_35_TM=59.481
PRIMER_RIGHT_35_TM=57.356
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.1000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=877
PRIMER_PAIR_36_PENALTY=4.177643
PRIMER_LEFT_36_PENALTY=3.606821
PRIMER_RIGHT_36_PENALTY=0.570821
PRIMER_LEFT_36_SEQUENCE=CGAGTATTCAATCACAACATCCC
PRIMER_RIGHT_36_SEQUENCE=CAAAAGGCCATAGTCCCAAC
PRIMER_LEFT_36=924,23
PRIMER_RIGHT_36=1894,20
PRIMER_LEFT_36_TM=60.607
PRIMER_RIGHT_36_TM=59.429
PRIMER_LEFT_36_GC_PERCENT=43.478
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.3000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=971
PRIMER_PAIR_37_PENALTY=4.177643
PRIMER_LEFT_37_PENALTY=3.606821
PRIMER_RIGHT_37_PENALTY=0.570821
PRIMER_LEFT_37_SEQUENCE=CGAGTATTCAATCACAACATCCC
PRIMER_RIGHT_37_SEQUENCE=ACAAAAGGCCATAGTCCCAA
PRIMER_LEFT_37=924,23
PRIMER_RIGHT_37=1895,20
PRIMER_LEFT_37_TM=60.607
PRIMER_RIGHT_37_TM=59.429
PRIMER_LEFT_37_GC_PERCENT=43.478
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.3000
PRIMER_RIGHT_37_END_STABILITY=10.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=972
PRIMER_PAIR_38_PENALTY=4.177643
PRIMER_LEFT_38_PENALTY=3.606821
PRIMER_RIGHT_38_PENALTY=0.570821
PRIMER_LEFT_38_SEQUENCE=CGAGTATTCAATCACAACATCCC
PRIMER_RIGHT_38_SEQUENCE=AACAAAAGGCCATAGTCCCA
PRIMER_LEFT_38=924,23
PRIMER_RIGHT_38=1896,20
PRIMER_LEFT_38_TM=60.607
PRIMER_RIGHT_38_TM=59.429
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.3000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=973
PRIMER_PAIR_39_PENALTY=4.236126
PRIMER_LEFT_39_PENALTY=1.621022
PRIMER_RIGHT_39_PENALTY=2.615104
PRIMER_LEFT_39_SEQUENCE=AATGGCAGAGCATAAGCAAAC
PRIMER_RIGHT_39_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_39=806,21
PRIMER_RIGHT_39=1687,22
PRIMER_LEFT_39_TM=59.379
PRIMER_RIGHT_39_TM=59.385
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=2.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=882
PRIMER_PAIR_40_PENALTY=4.241966
PRIMER_LEFT_40_PENALTY=1.754714
PRIMER_RIGHT_40_PENALTY=2.487253
PRIMER_LEFT_40_SEQUENCE=GCAGAGCATAAGCAAACCCTT
PRIMER_RIGHT_40_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_40=810,21
PRIMER_RIGHT_40=1691,22
PRIMER_LEFT_40_TM=60.755
PRIMER_RIGHT_40_TM=59.513
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=882
PRIMER_PAIR_41_PENALTY=4.250565
PRIMER_LEFT_41_PENALTY=1.125045
PRIMER_RIGHT_41_PENALTY=3.125520
PRIMER_LEFT_41_SEQUENCE=TCGGTGTGTTTACTTATGCCC
PRIMER_RIGHT_41_SEQUENCE=CAAGTCACAGTCAACGAACAAA
PRIMER_LEFT_41=751,21
PRIMER_RIGHT_41=1686,22
PRIMER_LEFT_41_TM=59.875
PRIMER_RIGHT_41_TM=58.874
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=11.2000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=936
PRIMER_PAIR_42_PENALTY=4.260986
PRIMER_LEFT_42_PENALTY=1.125045
PRIMER_RIGHT_42_PENALTY=3.135941
PRIMER_LEFT_42_SEQUENCE=TCGGTGTGTTTACTTATGCCC
PRIMER_RIGHT_42_SEQUENCE=ACTCAAGTCACAGTCAACGAACA
PRIMER_LEFT_42=751,21
PRIMER_RIGHT_42=1689,23
PRIMER_LEFT_42_TM=59.875
PRIMER_RIGHT_42_TM=59.864
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=11.2000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=939
PRIMER_PAIR_43_PENALTY=4.260986
PRIMER_LEFT_43_PENALTY=1.125045
PRIMER_RIGHT_43_PENALTY=3.135941
PRIMER_LEFT_43_SEQUENCE=TCGGTGTGTTTACTTATGCCC
PRIMER_RIGHT_43_SEQUENCE=CACTCAAGTCACAGTCAACGAAC
PRIMER_LEFT_43=751,21
PRIMER_RIGHT_43=1690,23
PRIMER_LEFT_43_TM=59.875
PRIMER_RIGHT_43_TM=59.864
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=47.826
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.2000
PRIMER_RIGHT_43_END_STABILITY=8.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=940
PRIMER_PAIR_44_PENALTY=4.267590
PRIMER_LEFT_44_PENALTY=1.125045
PRIMER_RIGHT_44_PENALTY=3.142546
PRIMER_LEFT_44_SEQUENCE=TCGGTGTGTTTACTTATGCCC
PRIMER_RIGHT_44_SEQUENCE=TCAAGTCACAGTCAACGAACA
PRIMER_LEFT_44=751,21
PRIMER_RIGHT_44=1687,21
PRIMER_LEFT_44_TM=59.875
PRIMER_RIGHT_44_TM=57.857
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=11.2000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=937
PRIMER_PAIR_45_PENALTY=4.313070
PRIMER_LEFT_45_PENALTY=1.825817
PRIMER_RIGHT_45_PENALTY=2.487253
PRIMER_LEFT_45_SEQUENCE=GCCCAAAGTTAGGTCAGGTT
PRIMER_RIGHT_45_SEQUENCE=TCACTCAAGTCACAGTCAACGA
PRIMER_LEFT_45=768,20
PRIMER_RIGHT_45=1691,22
PRIMER_LEFT_45_TM=58.174
PRIMER_RIGHT_45_TM=59.513
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=924
PRIMER_PAIR_46_PENALTY=4.321790
PRIMER_LEFT_46_PENALTY=1.303996
PRIMER_RIGHT_46_PENALTY=3.017794
PRIMER_LEFT_46_SEQUENCE=TGGCAGAGCATAAGCAAACC
PRIMER_RIGHT_46_SEQUENCE=CTCAAGTCACAGTCAACGAACA
PRIMER_LEFT_46=808,20
PRIMER_RIGHT_46=1688,22
PRIMER_LEFT_46_TM=61.304
PRIMER_RIGHT_46_TM=58.982
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=881
PRIMER_PAIR_47_PENALTY=4.321790
PRIMER_LEFT_47_PENALTY=1.303996
PRIMER_RIGHT_47_PENALTY=3.017794
PRIMER_LEFT_47_SEQUENCE=TGGCAGAGCATAAGCAAACC
PRIMER_RIGHT_47_SEQUENCE=CACTCAAGTCACAGTCAACGAA
PRIMER_LEFT_47=808,20
PRIMER_RIGHT_47=1690,22
PRIMER_LEFT_47_TM=61.304
PRIMER_RIGHT_47_TM=58.982
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=883
PRIMER_PAIR_48_PENALTY=4.369818
PRIMER_LEFT_48_PENALTY=1.754714
PRIMER_RIGHT_48_PENALTY=2.615104
PRIMER_LEFT_48_SEQUENCE=GCAGAGCATAAGCAAACCCTT
PRIMER_RIGHT_48_SEQUENCE=TCAAGTCACAGTCAACGAACAA
PRIMER_LEFT_48=810,21
PRIMER_RIGHT_48=1687,22
PRIMER_LEFT_48_TM=60.755
PRIMER_RIGHT_48_TM=59.385
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=878
PRIMER_PAIR_49_PENALTY=4.378919
PRIMER_LEFT_49_PENALTY=0.468413
PRIMER_RIGHT_49_PENALTY=3.910507
PRIMER_LEFT_49_SEQUENCE=GCAAACCCTTGAGACAATCC
PRIMER_RIGHT_49_SEQUENCE=TCACTCAAGTCACAGTCAACGAA
PRIMER_LEFT_49=821,20
PRIMER_RIGHT_49=1691,23
PRIMER_LEFT_49_TM=59.532
PRIMER_RIGHT_49_TM=60.911
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=43.478
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=871
=
SEQUENCE_ID=MAGIC.329_3_9012945_9013062_zu-0_ler-0_520796_2895_TRUE
SEQUENCE_TARGET=950,217
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_5_12774877_12775011_bur-0_wil-2_21997_218475_TRUE
SEQUENCE_TARGET=950,234
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_4414020_4414215_ler-0_sf-2_174709_244314_TRUE
SEQUENCE_TARGET=950,295
SEQUENCE_TEMPLATE=CGCATTCTAGGGACCTACAGAATATTCCTCAATTTTAATCAACACTTGAATNGTTGNTTAGTCCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_15200849_15201478_rsch-4_zu-0_318729_191796_TRUE
SEQUENCE_TARGET=950,729
SEQUENCE_TEMPLATE=ATTATTAAAGAACCCAATATATATATTCCTCGATGATTGATCTAGCTTTTGAAGTTTCCTTTAGTTCTAACTAATGAGGATATAAAGAAGGAGCACTATCAAGTTTGAATTACAAAGGACTCAAATTAGGAACCAAGCCGCACTTCCCGTCCAAGAACGTCACAACAAAATTAAGCTTGACACTCTTAAACAATTATTGCATCTCTATTTTTACTTTATTCTCATTTTGATTCGTCAAATATAGCAATGACAGATTCAAAAAATCAGTNCATTTCTACAGCTAAAATACTTNCAAGATNATTAGAATCATGTAAGTATAACCACAAGCATGGATAAAACAGAACCNAAGTTAAACCAATTCATCAAAATGACTAAACAAACTGGCCCCTTTTCATTTAAACCGGAACAATTCTTGTTATTCATTTAAGAATTAACTGGTAGTTTTTGTAAAAAAATGCAATTTATTAGATGAATAATGTCATTCGTTTTAATAGAATGTTCATTGCAATGCTGTCATAAATGAAATAAATATGCCCATTCATTTTTTCTGCGTTTGGAAAGTGGAAGAATTTTTCAAGAGGATCGGTCATTCATGCATGTAATGATGCAACGGAATAAGAATAACCAAACATTCGATCGAAAGTACTTAAATACCTAAATGTGCTTGTCTTAAAATATATCTAAGATATAGGATTCAAGATATCTGAATATCATATATAAAAAATGAACATACAGATACAGTGNATATTTGAATTTATGAAATATGGGATTNCTCGATTGGAAAAGTACCATAGTCCATTAGTCCGAGTAAAGGTTTTAAAAGCTTATCCGAATCTAAAGAAATGTACGAATTTTGGCTTANAANCTCTTAATTACAACAAAAAAAAGTTATTAGGTANTAAATTTTTCACCTGATATTGGTGATTTGCTATATACATCTGATNTAGATANAAAACAAAAGTTATACTTTGAGATAAATCCAAAACCCTATATTCTGGGNNTCTATAAATGTTATTAATTACCGTCTNTTTNTANCGCTTACTCCACACAAAATTGTAATATTTNAAAGATTTAAAAACACCAAAAAGAGATAAGTTAGTGTGAATTGTGGAAGTAACAGCTAGAATTCCAACGTGAAGGACGATAGTGAATGAAACACCCAAAACCAATTAAACTTAGTCATGATGAGAGGGATGCATGAAACTAATCGGCGATGGTTAAAGGTCAGCCTCATCTCATGTCTGTGTCTTATCTCATATATGATAATCACCCACAATATCTGTATATGGTCCTCGTCAGATTTCACTCATTTTGTATCCTCNAGAAATTATGCAGTACTACTAGTTACTTATTGGCAGATTTTTTATTGCTCCATTTCCATAGTATATATAATATCATTTAATTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGATATATTAGTNTTGTCTATCAACATTTATCTGTCGGATTCACCAGGTNTTAGGAAAGAAGCCCGACTATTTGTTTCGAAATTTTATTGTATGTCACATGAATACGTTTCAACGTGATGCATATGTTATTAATGTATACATAGATACATACATCATGTNGATATCCTTTCAAGAATATCTAGATCATAAATTCATAATAGATATGTTCATAGCTAGAATTAGAAGAATACTTTACTAGAAAATGAAATGAAAATGATTAGCNATCTGATAAAATGNNNNAAAAAAAAAAGTCTACTGAAGGAGGATGANAAAGTTTAAGTGTTGAGATTTTATTCAAGTTATGTAGTATACAAAAAAGGGGATAGTTTTAGACTTTTAGTAGTACGAATAGCTAATCTTCTAAATTAATTGCTATCTATTANACTACACTATCAATAACTAAAAATGAATAAATCTAGTGCAGTTTATTCAATAAAAATGTCACGAATTTTACATAAGTTGTNNNNNNNNNNNTATTAAAACATAANCACACAACATCTATTTTGTTGGAATACCATAAACTTTTACTTGACGGCTACATANACATGATACATGATTAACATAGGCCTTTTNAAAAAGCAAATATATATGACTAGAAGAAGAAAGCAAAAGAAATGGTAGAAGACAGAAATCAAAAGCGTGCAAGAATTATATATACTATGATATGGGCTCCAACTAGAGATCACTTGTTGACAAAGAAGAAGAAAAAAAGAGAGAGAGATCACTTTGGAAATAACAATGGCGAACAAAAGGACAAATATATGCCACGTCATCCCTCTCCATCCACCTTTATGTCATTTCTCAAAAAAACAAANTTTTTTTATCCGATTCATTATNAANAAANNAANNNNNNNAAAAACAATTATTTTAAATTATTTGGTTAGTGTTTCATTATAATTTTTCTTATAAATAGGGTCTTTAAACCTTCCAANAAGTCATATANAAAACNGCTTCATAAAAAGAAGATAGAACTTGGATAAGTTTAGAAGATAGAAATGGAATGGAGAGAATGTTATTTAGATGTGATATTGGTGCCGCTAGGGCTAATGGTGTATGCATCTTACCATGTTTACTTGTGGCATNAGTTACGAACACAGCCTCTCACCACTATCATCGGCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_9016163_9016255_hi-0_ct-1_1514_83982_TRUE
SEQUENCE_TARGET=950,192
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATANTGAATATAGNTACNAAATAATATAATGATANATTATAATATGACAAATCACAATTTAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_23019105_23019133_wu-0_can-0_424224_561_TRUE
SEQUENCE_TARGET=950,128
SEQUENCE_TEMPLATE=TTGACAAGCTTGCTTGGATCATCACTTTTGACTGGGTCGAGTGTTTTCGCNCACGAACCTTGGTCTGTGGTTGACTGGCAAATTGTCTGAACCGCTTTCTGATGAGAGTTTATGGGACCATTACTNTCGCCNTTGGCGCCGCCTCCTTTATTAACGAACGTGATAACTCCTATGGCAACACCCACCACTAGGAGGAGTGATGCCACAGATANCACAACCTTTCCTACTGCCATTTTTTCTAACNNTTTTTTTTTGTTNTTATATATTTTTCCCNTTTTTGATTTTNNNTTTTTTTTTNNTTTTTATNTTTTTTCTCTCCCTCCGGTTTTATTCGGGAGGTAAATANGNGAGGGAGGGGATTTTTTTTATTGAAACAGAGCCCGGTGGTTTATTAATGGGGCTGGTAGAGATTGTGGTGCATTGTGGAGACATTCTTATAGGATTTGTAGTTTCTAAAATTAGGGTTTGCATGGAGCTAGGGAGAGATTTAAGNTCGTTGAATGGCTAAAAATTCTCTAATNNAATATTGGCTATGAGACTAAAAATNGGTCACCACAAGATGATTTGATTATGTCCTGATCAAGAGTTCGATTACTAAAAATATNAAACGTAGATCCAANCAAAAAAANTGTATGTGGCAGTAACTCTAGTTTACTNNTACATATAACATATCCTCTNATTCTAATCTTGTGAATNTATTTTGGANTATTCATTCCTAAGTTTAGTNATTTATCCGAAAGTAAAGTATTCGAAAACTTCTTATTGAGAGTTTNCTCTCATCCTTTTAAGGTTAAAACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTATAACGAAAGGATTATGAGCTTNAAGATTCAAAATTAGTTTTGNTTAAAGTGTTTGAATAGAAAAACCGAANTNGTAAGCNTTTATTCAATAGAAANNNNNAAAAAANCGAAATCATATGATACNNNNNNNNTNANNCANATNTNNNTNNACNTNNNNCNANNNNNTGNNCNANNNNATNNGNTANNNTNTNCGNTNAACNNTCNNNNNNCTNNNATNNTANNATTGATGACGCAAAACGGTAAAAAATACNAAAATAGCAATAACGATTANGGAATGATGATAATAAAAAAGAGAGAGANAATTTGCATCATAGATATCACGANATTAGAGAGATTTAATTNNNNNNNNNNNNNTNNTNNTNNNNNNNGGTTAACNGTTAANNNNNNGTNAATTGTCAANNNNCAANAACNGNANNTNNNTNANNNANNCNCNNTNATNTATATANANANNAAANNAACTNNNNACNCGNATNNACNNTNGANTNTGNNNANNTTNAAATGAGATTAGCAGATAATATAANTTTTTTGCAGATTTCAAAATTTCCTGAGGCTAAATGNCTAAATCATCATTCTAATACTTTNGTTATCATTAACNANATGTATTTAAAANAAAAATGTTGAAANAGNGACAAAAAATAATAACATTTCATTAATTAATATATCCTTTCCAATCATCACGAAATCCGTCCGTNCATNTATAAAACCTACAACATTACATCACAAACGACGCATAATCATACATGACAGAACCAACCATCTATGATTCCATTCGCGATTGCTCTAGGCCTTNNACGATATAATTAATTATTACATCCAATCCCTTAATACGATCATCAGTAATAATAATCAGACAAGGGTGGCGGTCGGGGCGAAGTGTCTTCCTCGTTAAGCTTCACCTTAATACCAAAAGTCTTGAAAATAGTATTCAAGTTATTAAACACATCAAAAGAATCACTTGTTAGATTCTTCCCAACTCCAATCCCAATCCCCACTTCCTTCAACAAAATCTTGTCTTCAATATCGTCCAAACAAGTCGAATGATACCCCATGATTGACATTAACTCTTTTTTACACGTAAAATAATTATGAGCCAACGTCGTTACATCTTTCCCCGTAGCTTTCCAAAAATCGGCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.143605
PRIMER_LEFT_0_PENALTY=0.032945
PRIMER_RIGHT_0_PENALTY=0.110660
PRIMER_LEFT_0_SEQUENCE=ATTGTGGTGCATTGTGGAGA
PRIMER_RIGHT_0_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_0=409,20
PRIMER_RIGHT_0=1136,20
PRIMER_LEFT_0_TM=59.967
PRIMER_RIGHT_0_TM=60.111
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=728
PRIMER_PAIR_1_PENALTY=0.143605
PRIMER_LEFT_1_PENALTY=0.032945
PRIMER_RIGHT_1_PENALTY=0.110660
PRIMER_LEFT_1_SEQUENCE=GATTGTGGTGCATTGTGGAG
PRIMER_RIGHT_1_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_1=408,20
PRIMER_RIGHT_1=1136,20
PRIMER_LEFT_1_TM=59.967
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=729
PRIMER_PAIR_2_PENALTY=0.143605
PRIMER_LEFT_2_PENALTY=0.032945
PRIMER_RIGHT_2_PENALTY=0.110660
PRIMER_LEFT_2_SEQUENCE=AGATTGTGGTGCATTGTGGA
PRIMER_RIGHT_2_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_2=407,20
PRIMER_RIGHT_2=1136,20
PRIMER_LEFT_2_TM=59.967
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=730
PRIMER_PAIR_3_PENALTY=0.143605
PRIMER_LEFT_3_PENALTY=0.032945
PRIMER_RIGHT_3_PENALTY=0.110660
PRIMER_LEFT_3_SEQUENCE=GAGATTGTGGTGCATTGTGG
PRIMER_RIGHT_3_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_3=406,20
PRIMER_RIGHT_3=1136,20
PRIMER_LEFT_3_TM=59.967
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=731
PRIMER_PAIR_4_PENALTY=0.157028
PRIMER_LEFT_4_PENALTY=0.046368
PRIMER_RIGHT_4_PENALTY=0.110660
PRIMER_LEFT_4_SEQUENCE=TTTTCTCTCCCTCCGGTTTT
PRIMER_RIGHT_4_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_4=309,20
PRIMER_RIGHT_4=1136,20
PRIMER_LEFT_4_TM=60.046
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=828
PRIMER_PAIR_5_PENALTY=0.157028
PRIMER_LEFT_5_PENALTY=0.046368
PRIMER_RIGHT_5_PENALTY=0.110660
PRIMER_LEFT_5_SEQUENCE=TTTTTCTCTCCCTCCGGTTT
PRIMER_RIGHT_5_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_5=308,20
PRIMER_RIGHT_5=1136,20
PRIMER_LEFT_5_TM=60.046
PRIMER_RIGHT_5_TM=60.111
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=829
PRIMER_PAIR_6_PENALTY=0.214545
PRIMER_LEFT_6_PENALTY=0.103885
PRIMER_RIGHT_6_PENALTY=0.110660
PRIMER_LEFT_6_SEQUENCE=CCCACCACTAGGAGGAGTGA
PRIMER_RIGHT_6_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_6=180,20
PRIMER_RIGHT_6=1136,20
PRIMER_LEFT_6_TM=60.104
PRIMER_RIGHT_6_TM=60.111
PRIMER_LEFT_6_GC_PERCENT=60.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=957
PRIMER_PAIR_7_PENALTY=0.260055
PRIMER_LEFT_7_PENALTY=0.149395
PRIMER_RIGHT_7_PENALTY=0.110660
PRIMER_LEFT_7_SEQUENCE=ACTCCTATGGCAACACCCAC
PRIMER_RIGHT_7_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_7=165,20
PRIMER_RIGHT_7=1136,20
PRIMER_LEFT_7_TM=59.851
PRIMER_RIGHT_7_TM=60.111
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=972
PRIMER_PAIR_8_PENALTY=0.274318
PRIMER_LEFT_8_PENALTY=0.163658
PRIMER_RIGHT_8_PENALTY=0.110660
PRIMER_LEFT_8_SEQUENCE=GTTTGCATGGAGCTAGGGAG
PRIMER_RIGHT_8_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_8=463,20
PRIMER_RIGHT_8=1136,20
PRIMER_LEFT_8_TM=59.836
PRIMER_RIGHT_8_TM=60.111
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=674
PRIMER_PAIR_9_PENALTY=0.455881
PRIMER_LEFT_9_PENALTY=0.345221
PRIMER_RIGHT_9_PENALTY=0.110660
PRIMER_LEFT_9_SEQUENCE=TGGTTTATTAATGGGGCTGG
PRIMER_RIGHT_9_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_9=384,20
PRIMER_RIGHT_9=1136,20
PRIMER_LEFT_9_TM=59.655
PRIMER_RIGHT_9_TM=60.111
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=753
PRIMER_PAIR_10_PENALTY=0.485750
PRIMER_LEFT_10_PENALTY=0.375090
PRIMER_RIGHT_10_PENALTY=0.110660
PRIMER_LEFT_10_SEQUENCE=AACTCCTATGGCAACACCCA
PRIMER_RIGHT_10_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_10=164,20
PRIMER_RIGHT_10=1136,20
PRIMER_LEFT_10_TM=60.375
PRIMER_RIGHT_10_TM=60.111
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=973
PRIMER_PAIR_11_PENALTY=0.605833
PRIMER_LEFT_11_PENALTY=0.495173
PRIMER_RIGHT_11_PENALTY=0.110660
PRIMER_LEFT_11_SEQUENCE=TTGCATGGAGCTAGGGAGAG
PRIMER_RIGHT_11_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_11=465,20
PRIMER_RIGHT_11=1136,20
PRIMER_LEFT_11_TM=60.495
PRIMER_RIGHT_11_TM=60.111
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=672
PRIMER_PAIR_12_PENALTY=0.685841
PRIMER_LEFT_12_PENALTY=0.575181
PRIMER_RIGHT_12_PENALTY=0.110660
PRIMER_LEFT_12_SEQUENCE=AACACCCACCACTAGGAGGA
PRIMER_RIGHT_12_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_12=176,20
PRIMER_RIGHT_12=1136,20
PRIMER_LEFT_12_TM=59.425
PRIMER_RIGHT_12_TM=60.111
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=961
PRIMER_PAIR_13_PENALTY=0.690988
PRIMER_LEFT_13_PENALTY=0.580328
PRIMER_RIGHT_13_PENALTY=0.110660
PRIMER_LEFT_13_SEQUENCE=TTGTGGTGCATTGTGGAGAC
PRIMER_RIGHT_13_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_13=410,20
PRIMER_RIGHT_13=1136,20
PRIMER_LEFT_13_TM=60.580
PRIMER_RIGHT_13_TM=60.111
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=727
PRIMER_PAIR_14_PENALTY=0.853641
PRIMER_LEFT_14_PENALTY=0.742980
PRIMER_RIGHT_14_PENALTY=0.110660
PRIMER_LEFT_14_SEQUENCE=ACAGAGCCCGGTGGTTTATT
PRIMER_RIGHT_14_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_14=373,20
PRIMER_RIGHT_14=1136,20
PRIMER_LEFT_14_TM=60.743
PRIMER_RIGHT_14_TM=60.111
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.2000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=764
PRIMER_PAIR_15_PENALTY=0.943653
PRIMER_LEFT_15_PENALTY=0.832993
PRIMER_RIGHT_15_PENALTY=0.110660
PRIMER_LEFT_15_SEQUENCE=CTATGGCAACACCCACCACT
PRIMER_RIGHT_15_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_15=169,20
PRIMER_RIGHT_15=1136,20
PRIMER_LEFT_15_TM=60.833
PRIMER_RIGHT_15_TM=60.111
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=968
PRIMER_PAIR_16_PENALTY=0.984230
PRIMER_LEFT_16_PENALTY=0.873570
PRIMER_RIGHT_16_PENALTY=0.110660
PRIMER_LEFT_16_SEQUENCE=TTTGCATGGAGCTAGGGAGA
PRIMER_RIGHT_16_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_16=464,20
PRIMER_RIGHT_16=1136,20
PRIMER_LEFT_16_TM=60.874
PRIMER_RIGHT_16_TM=60.111
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=673
PRIMER_PAIR_17_PENALTY=1.092922
PRIMER_LEFT_17_PENALTY=0.982262
PRIMER_RIGHT_17_PENALTY=0.110660
PRIMER_LEFT_17_SEQUENCE=GGGCTGGTAGAGATTGTGGT
PRIMER_RIGHT_17_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_17=397,20
PRIMER_RIGHT_17=1136,20
PRIMER_LEFT_17_TM=59.018
PRIMER_RIGHT_17_TM=60.111
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=740
PRIMER_PAIR_18_PENALTY=1.104498
PRIMER_LEFT_18_PENALTY=0.993838
PRIMER_RIGHT_18_PENALTY=0.110660
PRIMER_LEFT_18_SEQUENCE=AATGGGGCTGGTAGAGATTG
PRIMER_RIGHT_18_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_18=393,20
PRIMER_RIGHT_18=1136,20
PRIMER_LEFT_18_TM=59.006
PRIMER_RIGHT_18_TM=60.111
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=744
PRIMER_PAIR_19_PENALTY=1.156627
PRIMER_LEFT_19_PENALTY=1.045967
PRIMER_RIGHT_19_PENALTY=0.110660
PRIMER_LEFT_19_SEQUENCE=TGCATGGAGCTAGGGAGAGA
PRIMER_RIGHT_19_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_19=466,20
PRIMER_RIGHT_19=1136,20
PRIMER_LEFT_19_TM=61.046
PRIMER_RIGHT_19_TM=60.111
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=671
PRIMER_PAIR_20_PENALTY=1.165616
PRIMER_LEFT_20_PENALTY=1.054956
PRIMER_RIGHT_20_PENALTY=0.110660
PRIMER_LEFT_20_SEQUENCE=TGGGGCTGGTAGAGATTGTG
PRIMER_RIGHT_20_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_20=395,20
PRIMER_RIGHT_20=1136,20
PRIMER_LEFT_20_TM=61.055
PRIMER_RIGHT_20_TM=60.111
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=742
PRIMER_PAIR_21_PENALTY=1.237327
PRIMER_LEFT_21_PENALTY=1.126667
PRIMER_RIGHT_21_PENALTY=0.110660
PRIMER_LEFT_21_SEQUENCE=CCGGTGGTTTATTAATGGGG
PRIMER_RIGHT_21_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_21=380,20
PRIMER_RIGHT_21=1136,20
PRIMER_LEFT_21_TM=61.127
PRIMER_RIGHT_21_TM=60.111
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=11.2000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=757
PRIMER_PAIR_22_PENALTY=1.237327
PRIMER_LEFT_22_PENALTY=1.126667
PRIMER_RIGHT_22_PENALTY=0.110660
PRIMER_LEFT_22_SEQUENCE=CCCGGTGGTTTATTAATGGG
PRIMER_RIGHT_22_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_22=379,20
PRIMER_RIGHT_22=1136,20
PRIMER_LEFT_22_TM=61.127
PRIMER_RIGHT_22_TM=60.111
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=758
PRIMER_PAIR_23_PENALTY=1.270023
PRIMER_LEFT_23_PENALTY=1.159363
PRIMER_RIGHT_23_PENALTY=0.110660
PRIMER_LEFT_23_SEQUENCE=AATGGGGCTGGTAGAGATTGT
PRIMER_RIGHT_23_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_23=393,21
PRIMER_RIGHT_23=1136,20
PRIMER_LEFT_23_TM=59.841
PRIMER_RIGHT_23_TM=60.111
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=744
PRIMER_PAIR_24_PENALTY=1.285829
PRIMER_LEFT_24_PENALTY=1.175168
PRIMER_RIGHT_24_PENALTY=0.110660
PRIMER_LEFT_24_SEQUENCE=GCATGGAGCTAGGGAGAGATT
PRIMER_RIGHT_24_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_24=467,21
PRIMER_RIGHT_24=1136,20
PRIMER_LEFT_24_TM=59.825
PRIMER_RIGHT_24_TM=60.111
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=670
PRIMER_PAIR_25_PENALTY=1.356907
PRIMER_LEFT_25_PENALTY=0.032945
PRIMER_RIGHT_25_PENALTY=1.323962
PRIMER_LEFT_25_SEQUENCE=ATTGTGGTGCATTGTGGAGA
PRIMER_RIGHT_25_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_25=409,20
PRIMER_RIGHT_25=1138,20
PRIMER_LEFT_25_TM=59.967
PRIMER_RIGHT_25_TM=58.676
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=730
PRIMER_PAIR_26_PENALTY=1.356907
PRIMER_LEFT_26_PENALTY=0.032945
PRIMER_RIGHT_26_PENALTY=1.323962
PRIMER_LEFT_26_SEQUENCE=GATTGTGGTGCATTGTGGAG
PRIMER_RIGHT_26_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_26=408,20
PRIMER_RIGHT_26=1138,20
PRIMER_LEFT_26_TM=59.967
PRIMER_RIGHT_26_TM=58.676
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=731
PRIMER_PAIR_27_PENALTY=1.356907
PRIMER_LEFT_27_PENALTY=0.032945
PRIMER_RIGHT_27_PENALTY=1.323962
PRIMER_LEFT_27_SEQUENCE=AGATTGTGGTGCATTGTGGA
PRIMER_RIGHT_27_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_27=407,20
PRIMER_RIGHT_27=1138,20
PRIMER_LEFT_27_TM=59.967
PRIMER_RIGHT_27_TM=58.676
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=732
PRIMER_PAIR_28_PENALTY=1.356907
PRIMER_LEFT_28_PENALTY=0.032945
PRIMER_RIGHT_28_PENALTY=1.323962
PRIMER_LEFT_28_SEQUENCE=GAGATTGTGGTGCATTGTGG
PRIMER_RIGHT_28_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_28=406,20
PRIMER_RIGHT_28=1138,20
PRIMER_LEFT_28_TM=59.967
PRIMER_RIGHT_28_TM=58.676
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.6000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=733
PRIMER_PAIR_29_PENALTY=1.370330
PRIMER_LEFT_29_PENALTY=0.046368
PRIMER_RIGHT_29_PENALTY=1.323962
PRIMER_LEFT_29_SEQUENCE=TTTTCTCTCCCTCCGGTTTT
PRIMER_RIGHT_29_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_29=309,20
PRIMER_RIGHT_29=1138,20
PRIMER_LEFT_29_TM=60.046
PRIMER_RIGHT_29_TM=58.676
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=830
PRIMER_PAIR_30_PENALTY=1.370330
PRIMER_LEFT_30_PENALTY=0.046368
PRIMER_RIGHT_30_PENALTY=1.323962
PRIMER_LEFT_30_SEQUENCE=TTTTTCTCTCCCTCCGGTTT
PRIMER_RIGHT_30_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_30=308,20
PRIMER_RIGHT_30=1138,20
PRIMER_LEFT_30_TM=60.046
PRIMER_RIGHT_30_TM=58.676
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=831
PRIMER_PAIR_31_PENALTY=1.427847
PRIMER_LEFT_31_PENALTY=0.103885
PRIMER_RIGHT_31_PENALTY=1.323962
PRIMER_LEFT_31_SEQUENCE=CCCACCACTAGGAGGAGTGA
PRIMER_RIGHT_31_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_31=180,20
PRIMER_RIGHT_31=1138,20
PRIMER_LEFT_31_TM=60.104
PRIMER_RIGHT_31_TM=58.676
PRIMER_LEFT_31_GC_PERCENT=60.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=959
PRIMER_PAIR_32_PENALTY=1.473357
PRIMER_LEFT_32_PENALTY=0.149395
PRIMER_RIGHT_32_PENALTY=1.323962
PRIMER_LEFT_32_SEQUENCE=ACTCCTATGGCAACACCCAC
PRIMER_RIGHT_32_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_32=165,20
PRIMER_RIGHT_32=1138,20
PRIMER_LEFT_32_TM=59.851
PRIMER_RIGHT_32_TM=58.676
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=6.6000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=974
PRIMER_PAIR_33_PENALTY=1.482228
PRIMER_LEFT_33_PENALTY=1.371568
PRIMER_RIGHT_33_PENALTY=0.110660
PRIMER_LEFT_33_SEQUENCE=TAACTCCTATGGCAACACCCA
PRIMER_RIGHT_33_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_33=163,21
PRIMER_RIGHT_33=1136,20
PRIMER_LEFT_33_TM=60.372
PRIMER_RIGHT_33_TM=60.111
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=974
PRIMER_PAIR_34_PENALTY=1.487620
PRIMER_LEFT_34_PENALTY=0.163658
PRIMER_RIGHT_34_PENALTY=1.323962
PRIMER_LEFT_34_SEQUENCE=GTTTGCATGGAGCTAGGGAG
PRIMER_RIGHT_34_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_34=463,20
PRIMER_RIGHT_34=1138,20
PRIMER_LEFT_34_TM=59.836
PRIMER_RIGHT_34_TM=58.676
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=6.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=676
PRIMER_PAIR_35_PENALTY=1.536508
PRIMER_LEFT_35_PENALTY=1.425848
PRIMER_RIGHT_35_PENALTY=0.110660
PRIMER_LEFT_35_SEQUENCE=TGGTTTATTAATGGGGCTGGT
PRIMER_RIGHT_35_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_35=384,21
PRIMER_RIGHT_35=1136,20
PRIMER_LEFT_35_TM=60.426
PRIMER_RIGHT_35_TM=60.111
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=753
PRIMER_PAIR_36_PENALTY=1.536508
PRIMER_LEFT_36_PENALTY=1.425848
PRIMER_RIGHT_36_PENALTY=0.110660
PRIMER_LEFT_36_SEQUENCE=GTGGTTTATTAATGGGGCTGG
PRIMER_RIGHT_36_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_36=383,21
PRIMER_RIGHT_36=1136,20
PRIMER_LEFT_36_TM=60.426
PRIMER_RIGHT_36_TM=60.111
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=754
PRIMER_PAIR_37_PENALTY=1.536508
PRIMER_LEFT_37_PENALTY=1.425848
PRIMER_RIGHT_37_PENALTY=0.110660
PRIMER_LEFT_37_SEQUENCE=GGTGGTTTATTAATGGGGCTG
PRIMER_RIGHT_37_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_37=382,21
PRIMER_RIGHT_37=1136,20
PRIMER_LEFT_37_TM=60.426
PRIMER_RIGHT_37_TM=60.111
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=755
PRIMER_PAIR_38_PENALTY=1.569923
PRIMER_LEFT_38_PENALTY=1.459263
PRIMER_RIGHT_38_PENALTY=0.110660
PRIMER_LEFT_38_SEQUENCE=TCTCTCCCTCCGGTTTTATTC
PRIMER_RIGHT_38_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_38=312,21
PRIMER_RIGHT_38=1136,20
PRIMER_LEFT_38_TM=59.541
PRIMER_RIGHT_38_TM=60.111
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=5.9000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=825
PRIMER_PAIR_39_PENALTY=1.637233
PRIMER_LEFT_39_PENALTY=1.526573
PRIMER_RIGHT_39_PENALTY=0.110660
PRIMER_LEFT_39_SEQUENCE=ATGGGGCTGGTAGAGATTGT
PRIMER_RIGHT_39_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_39=394,20
PRIMER_RIGHT_39=1136,20
PRIMER_LEFT_39_TM=58.473
PRIMER_RIGHT_39_TM=60.111
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=743
PRIMER_PAIR_40_PENALTY=1.669183
PRIMER_LEFT_40_PENALTY=0.345221
PRIMER_RIGHT_40_PENALTY=1.323962
PRIMER_LEFT_40_SEQUENCE=TGGTTTATTAATGGGGCTGG
PRIMER_RIGHT_40_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_40=384,20
PRIMER_RIGHT_40=1138,20
PRIMER_LEFT_40_TM=59.655
PRIMER_RIGHT_40_TM=58.676
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=755
PRIMER_PAIR_41_PENALTY=1.699052
PRIMER_LEFT_41_PENALTY=0.375090
PRIMER_RIGHT_41_PENALTY=1.323962
PRIMER_LEFT_41_SEQUENCE=AACTCCTATGGCAACACCCA
PRIMER_RIGHT_41_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_41=164,20
PRIMER_RIGHT_41=1138,20
PRIMER_LEFT_41_TM=60.375
PRIMER_RIGHT_41_TM=58.676
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=6.6000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=975
PRIMER_PAIR_42_PENALTY=1.819135
PRIMER_LEFT_42_PENALTY=0.495173
PRIMER_RIGHT_42_PENALTY=1.323962
PRIMER_LEFT_42_SEQUENCE=TTGCATGGAGCTAGGGAGAG
PRIMER_RIGHT_42_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_42=465,20
PRIMER_RIGHT_42=1138,20
PRIMER_LEFT_42_TM=60.495
PRIMER_RIGHT_42_TM=58.676
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=674
PRIMER_PAIR_43_PENALTY=1.899142
PRIMER_LEFT_43_PENALTY=0.575181
PRIMER_RIGHT_43_PENALTY=1.323962
PRIMER_LEFT_43_SEQUENCE=AACACCCACCACTAGGAGGA
PRIMER_RIGHT_43_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_43=176,20
PRIMER_RIGHT_43=1138,20
PRIMER_LEFT_43_TM=59.425
PRIMER_RIGHT_43_TM=58.676
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=963
PRIMER_PAIR_44_PENALTY=1.904290
PRIMER_LEFT_44_PENALTY=0.580328
PRIMER_RIGHT_44_PENALTY=1.323962
PRIMER_LEFT_44_SEQUENCE=TTGTGGTGCATTGTGGAGAC
PRIMER_RIGHT_44_SEQUENCE=TTTTACCGTTTTGCGTCATC
PRIMER_LEFT_44=410,20
PRIMER_RIGHT_44=1138,20
PRIMER_LEFT_44_TM=60.580
PRIMER_RIGHT_44_TM=58.676
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.1000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=729
PRIMER_PAIR_45_PENALTY=1.915369
PRIMER_LEFT_45_PENALTY=1.804709
PRIMER_RIGHT_45_PENALTY=0.110660
PRIMER_LEFT_45_SEQUENCE=GGGGCTGGTAGAGATTGTGG
PRIMER_RIGHT_45_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_45=396,20
PRIMER_RIGHT_45=1136,20
PRIMER_LEFT_45_TM=61.805
PRIMER_RIGHT_45_TM=60.111
PRIMER_LEFT_45_GC_PERCENT=60.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=741
PRIMER_PAIR_46_PENALTY=1.949164
PRIMER_LEFT_46_PENALTY=1.838504
PRIMER_RIGHT_46_PENALTY=0.110660
PRIMER_LEFT_46_SEQUENCE=ATTGTGGTGCATTGTGGAGAC
PRIMER_RIGHT_46_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_46=409,21
PRIMER_RIGHT_46=1136,20
PRIMER_LEFT_46_TM=60.839
PRIMER_RIGHT_46_TM=60.111
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.1000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=728
PRIMER_PAIR_47_PENALTY=1.952497
PRIMER_LEFT_47_PENALTY=1.841837
PRIMER_RIGHT_47_PENALTY=0.110660
PRIMER_LEFT_47_SEQUENCE=GGCTGGTAGAGATTGTGGTG
PRIMER_RIGHT_47_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_47=398,20
PRIMER_RIGHT_47=1136,20
PRIMER_LEFT_47_TM=58.158
PRIMER_RIGHT_47_TM=60.111
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=739
PRIMER_PAIR_48_PENALTY=2.032487
PRIMER_LEFT_48_PENALTY=1.921827
PRIMER_RIGHT_48_PENALTY=0.110660
PRIMER_LEFT_48_SEQUENCE=TTCTCTCCCTCCGGTTTTATT
PRIMER_RIGHT_48_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_48=311,21
PRIMER_RIGHT_48=1136,20
PRIMER_LEFT_48_TM=59.078
PRIMER_RIGHT_48_TM=60.111
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.2000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=826
PRIMER_PAIR_49_PENALTY=2.057098
PRIMER_LEFT_49_PENALTY=1.946438
PRIMER_RIGHT_49_PENALTY=0.110660
PRIMER_LEFT_49_SEQUENCE=TAATGGGGCTGGTAGAGATTG
PRIMER_RIGHT_49_SEQUENCE=TTACCGTTTTGCGTCATCAA
PRIMER_LEFT_49=392,21
PRIMER_RIGHT_49=1136,20
PRIMER_LEFT_49_TM=59.054
PRIMER_RIGHT_49_TM=60.111
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=745
=
SEQUENCE_ID=MAGIC.287_3_9016163_9016255_hi-0_ct-1_1505_288270_TRUE
SEQUENCE_TARGET=950,192
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATANTGAATATAGNTACNAAATAATATAATGATANATTATAATATGACAAATCACAATTTAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_6001281_6001299_po-0_oy-0_68033_162843_TRUE
SEQUENCE_TARGET=950,118
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATANGTGGGTTATGAGAAGCCGAGATAGCGTGGATAGCGTGGTCAAGGATTCTTTGACATATTGAGGAACAATATTCGTGGGTTATGAGAAGCCGAGATAACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANNNNNNGNTAANGANATTTACTTATTCGATTCATAAGTACCTATCTTATTCTTTGACATATTCGTAAAATTAAAGTTATCATGAATTGATTCTTANTTTTCAACCAAGAATACANTTGAATCCAACCAAATTATTTCATAACAAATCTACAAATCCAACCAAACCTAAACTCAACTATACTAAGATGATATATTCGTAGTATTCAATCTANATAATCAAAATATGAATGTAANAAACNGTGAATTCTTATGTGACATAGNTGGATTTTAAGTTCTTATAAACCCAATCCGAGCTGAACAAAAATAAAATCAAACTTAAACTTAACTTTTTAAAAAGAAAAAANAACTTTGTACCAAATNTATGTACAATATATTTTGTTTTACATTNATAAACTTTTNAAAATACTCAAAGTTTCCTAAACTTTTATTTCTAATGAACNTTTNATTNTGGTTACTGTGCTAATTAGCTTAGTTTAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_22752954_22752988_edi-0_can-0_2153819_164_TRUE
SEQUENCE_TARGET=950,134
SEQUENCE_TEMPLATE=AACCTTCACGTAAAAGATTTTCAAAAGAAAATCTNTANANGATATCAATATATAAAGGATATCAATATAAAACGGANATATTTTGATTCATGATAGTAGAGAATATNTAAACTCTTTGGAGGAAAATAAGCCGTGTGTCTTTGTNATACTCGNAANCTTAAANNATATTAGAATCAANGTGATTTATTTATAGNAAAAGGAATAAGGCTTTTAAGCTTCAATCAAACGAAGCTTATAGCTGAAGATATTAAGAACTTGTTGTATTCACATAAAGCAAAAAAGANGAAACTTGTTCGTCCTTTATTGTACATCTNAAAGACTAAANATTTATGCTCANAATGTTTTCTTTTGCCTTTTATATTCATATTACATTATACATGATCCTCCTTTCTTTTGGCGCATGGCATATTTTGTCAAACTCTATTACAACATTGATTCTAATCGATCTACAGTTGAAACTAGTGAATCGAATTNTCAGGTAGAAGTTAAATTTGATTTGANATGTNNTCAAGTTTATGCTANCTTTCTCATATAATATCCAATATTTTAGACNAGTTATATGTTGTTTATGTCGAAGATTNTTATTTGTTAGGTACGANAAATTTATATGAGCCACCANATATATAGTTGTAATCGACCATTTTCTNTAATTTNTTGGTTAAGATTTAAGAAGACTAGAAGAGGGACCGATGTCACTAGATNCACAAACTTAGCNACNTANNTTTACATGAATCCAAGTTACATATTAAACCAATGNCAAGAGCCGAAGCACCTCATGAATCCAAAAAATCTACGTAGGACGGTAACTTTTGTTGATCNTGAGACAAAGGATNTAGCCGAATATGANTNGCAACCAANTNTTAACTTCTCTCTATCAACGACGATAATCTTNGTTGCAACATGAAAAACATTCATAAACAAACATTTGCCGGTTCTCCTTTAGTCGCGGAATGATCTCTTTCCNATGGCCAGAGACATGTTGTGGTAANCNAGCCTAGTANTTCNNNNNTTTATGGTTCCGAGAACNATNTTTGNNNNTNTNGANTNACCNNTCCANNNNTTNANTNTNNANNNTNANTCNATNTTCACTNTTTNNANTNTNGAATCNTGANTCNANNCNATGNTNTNNNNNTTTGTTTGTACTTTGGAGAAAACCAACNNNNATTTCCAATAGGGCATAATTTGAGATNNNNNTNTTNANTNATAAATACGTACTAATCATGCATGGGACTTNTTGAGTATTTTATCGATATTNAGATCTNANGTANNNATTCTGTTCTTGCATTGCATNTATTTTTNAGTGGCATTTGATAGATTAGACACACATATATGTCTTTATAGTAANATATGAATAATNTTTTTTCTGTAAACATNAATGATGTTGAATNCAAATNAGTATATATTTATCGGTATCTATACTAAAATCATATATGTATATTCAAATCATAAAGTAGGAAGCAGTGTGGGCCGCTATTCTACTGGCTTCCACTNCTGAATTTCCATAATATATAGTTCTTAATCGAACCAACAANAAGTTGANTCCNNATGGANACATATTTATACATGGATATGTACGAACATAAATACATGCATGTANNCAGAAAATGNNNNNNNNTGCAAGTTTGGACATAANNNNNNNNATGTAANAGTAACGGCACTATCACNAACAATTAGGTTNTAAGTGTTGNTTTTNTGATTGTTAAANTTANAATTTATNATTNANNATGAAAAAAACAANTNTATAAAAACAACNAAGAACTTCATATGACAAACCACCAATTATTATGTTCTCTAACTTTAATAAGTGANTAACAAAGATGTTACCAACGACAAAAATATGNNNNTATATATATTCAAGAACAATGACAGGAACAAAAGGTTNTTCAACATAAAAACAACCCATATATGCTAAATTCAGCAATAAACCCCCCTTAAAATNTTNAAAGAAGAAGAAAATACATTNAAAATTATTGTTATGCTTTAGAGTCTCCAAGCTGAATTTAGCATATGTTCCCANCCGTCTTGACAACCACA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.665153
PRIMER_LEFT_0_PENALTY=0.022341
PRIMER_RIGHT_0_PENALTY=0.642812
PRIMER_LEFT_0_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_0_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_0=917,20
PRIMER_RIGHT_0=1467,20
PRIMER_LEFT_0_TM=59.978
PRIMER_RIGHT_0_TM=59.357
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=551
PRIMER_PAIR_1_PENALTY=0.797691
PRIMER_LEFT_1_PENALTY=0.154878
PRIMER_RIGHT_1_PENALTY=0.642812
PRIMER_LEFT_1_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_1_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_1=915,20
PRIMER_RIGHT_1=1467,20
PRIMER_LEFT_1_TM=59.845
PRIMER_RIGHT_1_TM=59.357
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=553
PRIMER_PAIR_2_PENALTY=0.799921
PRIMER_LEFT_2_PENALTY=0.022341
PRIMER_RIGHT_2_PENALTY=0.777580
PRIMER_LEFT_2_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_2_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_2=917,20
PRIMER_RIGHT_2=1478,20
PRIMER_LEFT_2_TM=59.978
PRIMER_RIGHT_2_TM=59.222
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=562
PRIMER_PAIR_3_PENALTY=0.799921
PRIMER_LEFT_3_PENALTY=0.022341
PRIMER_RIGHT_3_PENALTY=0.777580
PRIMER_LEFT_3_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_3_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_3=917,20
PRIMER_RIGHT_3=1479,20
PRIMER_LEFT_3_TM=59.978
PRIMER_RIGHT_3_TM=59.222
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=563
PRIMER_PAIR_4_PENALTY=0.932458
PRIMER_LEFT_4_PENALTY=0.154878
PRIMER_RIGHT_4_PENALTY=0.777580
PRIMER_LEFT_4_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_4_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_4=915,20
PRIMER_RIGHT_4=1478,20
PRIMER_LEFT_4_TM=59.845
PRIMER_RIGHT_4_TM=59.222
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=564
PRIMER_PAIR_5_PENALTY=0.932458
PRIMER_LEFT_5_PENALTY=0.154878
PRIMER_RIGHT_5_PENALTY=0.777580
PRIMER_LEFT_5_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_5_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_5=915,20
PRIMER_RIGHT_5=1479,20
PRIMER_LEFT_5_TM=59.845
PRIMER_RIGHT_5_TM=59.222
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=565
PRIMER_PAIR_6_PENALTY=1.070526
PRIMER_LEFT_6_PENALTY=0.022341
PRIMER_RIGHT_6_PENALTY=1.048185
PRIMER_LEFT_6_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_6_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_6=917,20
PRIMER_RIGHT_6=1486,20
PRIMER_LEFT_6_TM=59.978
PRIMER_RIGHT_6_TM=58.952
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=11.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=570
PRIMER_PAIR_7_PENALTY=1.167122
PRIMER_LEFT_7_PENALTY=0.524309
PRIMER_RIGHT_7_PENALTY=0.642812
PRIMER_LEFT_7_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_7_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_7=916,20
PRIMER_RIGHT_7=1467,20
PRIMER_LEFT_7_TM=59.476
PRIMER_RIGHT_7_TM=59.357
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=5.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=552
PRIMER_PAIR_8_PENALTY=1.187388
PRIMER_LEFT_8_PENALTY=0.022341
PRIMER_RIGHT_8_PENALTY=1.165047
PRIMER_LEFT_8_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_8_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_8=917,20
PRIMER_RIGHT_8=1479,21
PRIMER_LEFT_8_TM=59.978
PRIMER_RIGHT_8_TM=60.165
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.1000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=563
PRIMER_PAIR_9_PENALTY=1.198616
PRIMER_LEFT_9_PENALTY=0.022341
PRIMER_RIGHT_9_PENALTY=1.176275
PRIMER_LEFT_9_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_9_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_9=917,20
PRIMER_RIGHT_9=1480,20
PRIMER_LEFT_9_TM=59.978
PRIMER_RIGHT_9_TM=61.176
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=564
PRIMER_PAIR_10_PENALTY=1.203064
PRIMER_LEFT_10_PENALTY=0.154878
PRIMER_RIGHT_10_PENALTY=1.048185
PRIMER_LEFT_10_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_10_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_10=915,20
PRIMER_RIGHT_10=1486,20
PRIMER_LEFT_10_TM=59.845
PRIMER_RIGHT_10_TM=58.952
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=11.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=572
PRIMER_PAIR_11_PENALTY=1.301889
PRIMER_LEFT_11_PENALTY=0.524309
PRIMER_RIGHT_11_PENALTY=0.777580
PRIMER_LEFT_11_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_11_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_11=916,20
PRIMER_RIGHT_11=1478,20
PRIMER_LEFT_11_TM=59.476
PRIMER_RIGHT_11_TM=59.222
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=563
PRIMER_PAIR_12_PENALTY=1.301889
PRIMER_LEFT_12_PENALTY=0.524309
PRIMER_RIGHT_12_PENALTY=0.777580
PRIMER_LEFT_12_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_12_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_12=916,20
PRIMER_RIGHT_12=1479,20
PRIMER_LEFT_12_TM=59.476
PRIMER_RIGHT_12_TM=59.222
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=564
PRIMER_PAIR_13_PENALTY=1.319926
PRIMER_LEFT_13_PENALTY=0.154878
PRIMER_RIGHT_13_PENALTY=1.165047
PRIMER_LEFT_13_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_13_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_13=915,20
PRIMER_RIGHT_13=1479,21
PRIMER_LEFT_13_TM=59.845
PRIMER_RIGHT_13_TM=60.165
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=6.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=565
PRIMER_PAIR_14_PENALTY=1.330776
PRIMER_LEFT_14_PENALTY=0.687963
PRIMER_RIGHT_14_PENALTY=0.642812
PRIMER_LEFT_14_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_14_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_14=925,20
PRIMER_RIGHT_14=1467,20
PRIMER_LEFT_14_TM=59.312
PRIMER_RIGHT_14_TM=59.357
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=5.4000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=543
PRIMER_PAIR_15_PENALTY=1.331154
PRIMER_LEFT_15_PENALTY=0.154878
PRIMER_RIGHT_15_PENALTY=1.176275
PRIMER_LEFT_15_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_15_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_15=915,20
PRIMER_RIGHT_15=1480,20
PRIMER_LEFT_15_TM=59.845
PRIMER_RIGHT_15_TM=61.176
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=566
PRIMER_PAIR_16_PENALTY=1.465543
PRIMER_LEFT_16_PENALTY=0.687963
PRIMER_RIGHT_16_PENALTY=0.777580
PRIMER_LEFT_16_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_16_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_16=925,20
PRIMER_RIGHT_16=1478,20
PRIMER_LEFT_16_TM=59.312
PRIMER_RIGHT_16_TM=59.222
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=5.4000
PRIMER_RIGHT_16_END_STABILITY=6.1000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=554
PRIMER_PAIR_17_PENALTY=1.465543
PRIMER_LEFT_17_PENALTY=0.687963
PRIMER_RIGHT_17_PENALTY=0.777580
PRIMER_LEFT_17_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_17_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_17=925,20
PRIMER_RIGHT_17=1479,20
PRIMER_LEFT_17_TM=59.312
PRIMER_RIGHT_17_TM=59.222
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=555
PRIMER_PAIR_18_PENALTY=1.498434
PRIMER_LEFT_18_PENALTY=0.855622
PRIMER_RIGHT_18_PENALTY=0.642812
PRIMER_LEFT_18_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_18_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_18=921,20
PRIMER_RIGHT_18=1467,20
PRIMER_LEFT_18_TM=60.856
PRIMER_RIGHT_18_TM=59.357
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=5.7000
PRIMER_PAIR_18_COMPL_ANY=2.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=547
PRIMER_PAIR_19_PENALTY=1.498434
PRIMER_LEFT_19_PENALTY=0.855622
PRIMER_RIGHT_19_PENALTY=0.642812
PRIMER_LEFT_19_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_19_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_19=920,20
PRIMER_RIGHT_19=1467,20
PRIMER_LEFT_19_TM=60.856
PRIMER_RIGHT_19_TM=59.357
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=2.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=548
PRIMER_PAIR_20_PENALTY=1.498434
PRIMER_LEFT_20_PENALTY=0.855622
PRIMER_RIGHT_20_PENALTY=0.642812
PRIMER_LEFT_20_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_20_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_20=919,20
PRIMER_RIGHT_20=1467,20
PRIMER_LEFT_20_TM=60.856
PRIMER_RIGHT_20_TM=59.357
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=5.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=549
PRIMER_PAIR_21_PENALTY=1.572494
PRIMER_LEFT_21_PENALTY=0.524309
PRIMER_RIGHT_21_PENALTY=1.048185
PRIMER_LEFT_21_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_21_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_21=916,20
PRIMER_RIGHT_21=1486,20
PRIMER_LEFT_21_TM=59.476
PRIMER_RIGHT_21_TM=58.952
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=11.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=571
PRIMER_PAIR_22_PENALTY=1.631074
PRIMER_LEFT_22_PENALTY=0.022341
PRIMER_RIGHT_22_PENALTY=1.608733
PRIMER_LEFT_22_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_22_SEQUENCE=GGCCCACACTGCTTCCTACT
PRIMER_LEFT_22=917,20
PRIMER_RIGHT_22=1468,20
PRIMER_LEFT_22_TM=59.978
PRIMER_RIGHT_22_TM=61.609
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=60.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=5.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=552
PRIMER_PAIR_23_PENALTY=1.633201
PRIMER_LEFT_23_PENALTY=0.855622
PRIMER_RIGHT_23_PENALTY=0.777580
PRIMER_LEFT_23_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_23_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_23=921,20
PRIMER_RIGHT_23=1478,20
PRIMER_LEFT_23_TM=60.856
PRIMER_RIGHT_23_TM=59.222
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=6.1000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=558
PRIMER_PAIR_24_PENALTY=1.633201
PRIMER_LEFT_24_PENALTY=0.855622
PRIMER_RIGHT_24_PENALTY=0.777580
PRIMER_LEFT_24_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_24_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_24=921,20
PRIMER_RIGHT_24=1479,20
PRIMER_LEFT_24_TM=60.856
PRIMER_RIGHT_24_TM=59.222
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=559
PRIMER_PAIR_25_PENALTY=1.633201
PRIMER_LEFT_25_PENALTY=0.855622
PRIMER_RIGHT_25_PENALTY=0.777580
PRIMER_LEFT_25_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_25_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_25=920,20
PRIMER_RIGHT_25=1478,20
PRIMER_LEFT_25_TM=60.856
PRIMER_RIGHT_25_TM=59.222
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.1000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=559
PRIMER_PAIR_26_PENALTY=1.633201
PRIMER_LEFT_26_PENALTY=0.855622
PRIMER_RIGHT_26_PENALTY=0.777580
PRIMER_LEFT_26_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_26_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_26=920,20
PRIMER_RIGHT_26=1479,20
PRIMER_LEFT_26_TM=60.856
PRIMER_RIGHT_26_TM=59.222
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=560
PRIMER_PAIR_27_PENALTY=1.633201
PRIMER_LEFT_27_PENALTY=0.855622
PRIMER_RIGHT_27_PENALTY=0.777580
PRIMER_LEFT_27_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_27_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_27=919,20
PRIMER_RIGHT_27=1478,20
PRIMER_LEFT_27_TM=60.856
PRIMER_RIGHT_27_TM=59.222
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.1000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=560
PRIMER_PAIR_28_PENALTY=1.633201
PRIMER_LEFT_28_PENALTY=0.855622
PRIMER_RIGHT_28_PENALTY=0.777580
PRIMER_LEFT_28_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_28_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_28=919,20
PRIMER_RIGHT_28=1479,20
PRIMER_LEFT_28_TM=60.856
PRIMER_RIGHT_28_TM=59.222
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=561
PRIMER_PAIR_29_PENALTY=1.689356
PRIMER_LEFT_29_PENALTY=0.524309
PRIMER_RIGHT_29_PENALTY=1.165047
PRIMER_LEFT_29_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_29_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_29=916,20
PRIMER_RIGHT_29=1479,21
PRIMER_LEFT_29_TM=59.476
PRIMER_RIGHT_29_TM=60.165
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.1000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=564
PRIMER_PAIR_30_PENALTY=1.697565
PRIMER_LEFT_30_PENALTY=0.022341
PRIMER_RIGHT_30_PENALTY=1.675224
PRIMER_LEFT_30_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_30_SEQUENCE=GCCCACACTGCTTCCTACTTT
PRIMER_LEFT_30=917,20
PRIMER_RIGHT_30=1467,21
PRIMER_LEFT_30_TM=59.978
PRIMER_RIGHT_30_TM=60.675
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=551
PRIMER_PAIR_31_PENALTY=1.700585
PRIMER_LEFT_31_PENALTY=0.524309
PRIMER_RIGHT_31_PENALTY=1.176275
PRIMER_LEFT_31_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_31_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_31=916,20
PRIMER_RIGHT_31=1480,20
PRIMER_LEFT_31_TM=59.476
PRIMER_RIGHT_31_TM=61.176
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=565
PRIMER_PAIR_32_PENALTY=1.708955
PRIMER_LEFT_32_PENALTY=1.066143
PRIMER_RIGHT_32_PENALTY=0.642812
PRIMER_LEFT_32_SEQUENCE=GAAGCACCTCATGAATCCAAA
PRIMER_RIGHT_32_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_32=765,21
PRIMER_RIGHT_32=1467,20
PRIMER_LEFT_32_TM=60.066
PRIMER_RIGHT_32_TM=59.357
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.8000
PRIMER_RIGHT_32_END_STABILITY=5.7000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=703
PRIMER_PAIR_33_PENALTY=1.736149
PRIMER_LEFT_33_PENALTY=0.687963
PRIMER_RIGHT_33_PENALTY=1.048185
PRIMER_LEFT_33_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_33_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_33=925,20
PRIMER_RIGHT_33=1486,20
PRIMER_LEFT_33_TM=59.312
PRIMER_RIGHT_33_TM=58.952
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=5.4000
PRIMER_RIGHT_33_END_STABILITY=11.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=562
PRIMER_PAIR_34_PENALTY=1.763612
PRIMER_LEFT_34_PENALTY=0.154878
PRIMER_RIGHT_34_PENALTY=1.608733
PRIMER_LEFT_34_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_34_SEQUENCE=GGCCCACACTGCTTCCTACT
PRIMER_LEFT_34=915,20
PRIMER_RIGHT_34=1468,20
PRIMER_LEFT_34_TM=59.845
PRIMER_RIGHT_34_TM=61.609
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=60.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=5.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=554
PRIMER_PAIR_35_PENALTY=1.809002
PRIMER_LEFT_35_PENALTY=1.166190
PRIMER_RIGHT_35_PENALTY=0.642812
PRIMER_LEFT_35_SEQUENCE=TTTGCCGGTTCTCCTTTAGT
PRIMER_RIGHT_35_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_35=924,20
PRIMER_RIGHT_35=1467,20
PRIMER_LEFT_35_TM=58.834
PRIMER_RIGHT_35_TM=59.357
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=5.7000
PRIMER_RIGHT_35_END_STABILITY=5.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=544
PRIMER_PAIR_36_PENALTY=1.830103
PRIMER_LEFT_36_PENALTY=0.154878
PRIMER_RIGHT_36_PENALTY=1.675224
PRIMER_LEFT_36_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_36_SEQUENCE=GCCCACACTGCTTCCTACTTT
PRIMER_LEFT_36=915,20
PRIMER_RIGHT_36=1467,21
PRIMER_LEFT_36_TM=59.845
PRIMER_RIGHT_36_TM=60.675
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=553
PRIMER_PAIR_37_PENALTY=1.843723
PRIMER_LEFT_37_PENALTY=1.066143
PRIMER_RIGHT_37_PENALTY=0.777580
PRIMER_LEFT_37_SEQUENCE=GAAGCACCTCATGAATCCAAA
PRIMER_RIGHT_37_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_37=765,21
PRIMER_RIGHT_37=1478,20
PRIMER_LEFT_37_TM=60.066
PRIMER_RIGHT_37_TM=59.222
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.8000
PRIMER_RIGHT_37_END_STABILITY=6.1000
PRIMER_PAIR_37_COMPL_ANY=2.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=714
PRIMER_PAIR_38_PENALTY=1.843723
PRIMER_LEFT_38_PENALTY=1.066143
PRIMER_RIGHT_38_PENALTY=0.777580
PRIMER_LEFT_38_SEQUENCE=GAAGCACCTCATGAATCCAAA
PRIMER_RIGHT_38_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_38=765,21
PRIMER_RIGHT_38=1479,20
PRIMER_LEFT_38_TM=60.066
PRIMER_RIGHT_38_TM=59.222
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.8000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=2.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=715
PRIMER_PAIR_39_PENALTY=1.853011
PRIMER_LEFT_39_PENALTY=0.687963
PRIMER_RIGHT_39_PENALTY=1.165047
PRIMER_LEFT_39_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_39_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_39=925,20
PRIMER_RIGHT_39=1479,21
PRIMER_LEFT_39_TM=59.312
PRIMER_RIGHT_39_TM=60.165
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.4000
PRIMER_RIGHT_39_END_STABILITY=6.1000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=555
PRIMER_PAIR_40_PENALTY=1.864239
PRIMER_LEFT_40_PENALTY=0.687963
PRIMER_RIGHT_40_PENALTY=1.176275
PRIMER_LEFT_40_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_40_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_40=925,20
PRIMER_RIGHT_40=1480,20
PRIMER_LEFT_40_TM=59.312
PRIMER_RIGHT_40_TM=61.176
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=5.4000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=556
PRIMER_PAIR_41_PENALTY=1.903807
PRIMER_LEFT_41_PENALTY=0.855622
PRIMER_RIGHT_41_PENALTY=1.048185
PRIMER_LEFT_41_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_41_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_41=921,20
PRIMER_RIGHT_41=1486,20
PRIMER_LEFT_41_TM=60.856
PRIMER_RIGHT_41_TM=58.952
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=11.4000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=566
PRIMER_PAIR_42_PENALTY=1.903807
PRIMER_LEFT_42_PENALTY=0.855622
PRIMER_RIGHT_42_PENALTY=1.048185
PRIMER_LEFT_42_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_42_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_42=920,20
PRIMER_RIGHT_42=1486,20
PRIMER_LEFT_42_TM=60.856
PRIMER_RIGHT_42_TM=58.952
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=11.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=567
PRIMER_PAIR_43_PENALTY=1.903807
PRIMER_LEFT_43_PENALTY=0.855622
PRIMER_RIGHT_43_PENALTY=1.048185
PRIMER_LEFT_43_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_43_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_43=919,20
PRIMER_RIGHT_43=1486,20
PRIMER_LEFT_43_TM=60.856
PRIMER_RIGHT_43_TM=58.952
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=11.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=568
PRIMER_PAIR_44_PENALTY=1.943769
PRIMER_LEFT_44_PENALTY=1.166190
PRIMER_RIGHT_44_PENALTY=0.777580
PRIMER_LEFT_44_SEQUENCE=TTTGCCGGTTCTCCTTTAGT
PRIMER_RIGHT_44_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_44=924,20
PRIMER_RIGHT_44=1478,20
PRIMER_LEFT_44_TM=58.834
PRIMER_RIGHT_44_TM=59.222
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=5.7000
PRIMER_RIGHT_44_END_STABILITY=6.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=555
PRIMER_PAIR_45_PENALTY=1.943769
PRIMER_LEFT_45_PENALTY=1.166190
PRIMER_RIGHT_45_PENALTY=0.777580
PRIMER_LEFT_45_SEQUENCE=TTTGCCGGTTCTCCTTTAGT
PRIMER_RIGHT_45_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_45=924,20
PRIMER_RIGHT_45=1479,20
PRIMER_LEFT_45_TM=58.834
PRIMER_RIGHT_45_TM=59.222
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=5.7000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=556
PRIMER_PAIR_46_PENALTY=2.020669
PRIMER_LEFT_46_PENALTY=0.855622
PRIMER_RIGHT_46_PENALTY=1.165047
PRIMER_LEFT_46_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_46_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_46=921,20
PRIMER_RIGHT_46=1479,21
PRIMER_LEFT_46_TM=60.856
PRIMER_RIGHT_46_TM=60.165
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.1000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=559
PRIMER_PAIR_47_PENALTY=2.020669
PRIMER_LEFT_47_PENALTY=0.855622
PRIMER_RIGHT_47_PENALTY=1.165047
PRIMER_LEFT_47_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_47_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_47=920,20
PRIMER_RIGHT_47=1479,21
PRIMER_LEFT_47_TM=60.856
PRIMER_RIGHT_47_TM=60.165
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.1000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=560
PRIMER_PAIR_48_PENALTY=2.020669
PRIMER_LEFT_48_PENALTY=0.855622
PRIMER_RIGHT_48_PENALTY=1.165047
PRIMER_LEFT_48_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_48_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_48=919,20
PRIMER_RIGHT_48=1479,21
PRIMER_LEFT_48_TM=60.856
PRIMER_RIGHT_48_TM=60.165
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.1000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=561
PRIMER_PAIR_49_PENALTY=2.031897
PRIMER_LEFT_49_PENALTY=0.855622
PRIMER_RIGHT_49_PENALTY=1.176275
PRIMER_LEFT_49_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_49_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_49=921,20
PRIMER_RIGHT_49=1480,20
PRIMER_LEFT_49_TM=60.856
PRIMER_RIGHT_49_TM=61.176
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=560
=
SEQUENCE_ID=MAGIC.175_2_3012013_3012127_sf-2_edi-0_8219_82984_TRUE
SEQUENCE_TARGET=950,214
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCNGGAGNAGACTNATTATCACNGGCTNCGCCGAGGATGATGTTTNGATATCGGACCNAAAAAGATGCAACTCAATGAGACTTTAAACGAACGCTCTCAAGAGTTGTGNTGNCTCTNAGATNTTTGGGAGAATAAAAACNCTAGTAAATATAAGATCCATTGAGACNCGACTCTTCANTTTTANCAGATAANGTTCNTNGCANNCCTTTATTAAATGAACAGTGNTCNACAAACNAAATNTCNNGAAANATTACNTTGNCGTCTGACACAATACTAGNCTAGCNGTAATGTNCATTTNNGAAATGATTTATAGGGGTATAGGNGTCATNANACACATCAACAATCANTGGGAACANTTGAAAAATGGACGACCTAATTNCNGAGTCTTCCATGTACATATAATGGTAATACCNTTCTAATAATGTCTATGACATTAGATGTTTGTGATGTGGTGGAGTTGTAAATGTGTCAAAAGTTCAACAAATGCCANTTTTTTGTCTAATAGNTTTGAAGTACATCTTCGTAACTAGNTTTTATTTAGNAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_2_6718875_6719060_hi-0_mt-0_110338_10393_TRUE
SEQUENCE_TARGET=950,285
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_15800681_15801225_ct-1_hi-0_313489_20719_TRUE
SEQUENCE_TARGET=950,644
SEQUENCE_TEMPLATE=ACATGGACCTCGGCNAGNAATATCTCCTCTCTACAATTATGATCTCANTTTGTGACAGATTNTANGNATTTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTAATCANATGGGNTTTTGGNAGANTCTTGNGATAGNTAATAACAAGCAAATTCTTCTTANTCAAATTTGACTGANANAACAAAAATACGAGAATAGCCAAAACATCATTNATCCAAAATAAGANATGCTTAANGANGTTCAACAACTTAATTAACTCCCAAAATAACAATGGTTCTCNAAAGTAAAAANTTNAAAAACTACAANAGCTTNAAAGCCANACCCATNGANGCCTTCATGTTTATATCANTAAGCATCAATCATGAAGTTGGTGCTTGATTAACGGTACTGTAGAAACGAGAAAACAAAATCAGAAACAACAGTNTTGTGTAATGATGTTAACACAATTCATAGAGTTTGGAATTTACCTTAATGAATCCAATCTCCTTAGCGTTGCTACGGAAACACTGTCTGCAGCAGTTCAAACCATACTTCCNGATCAGNCCGTGCGAGTTNCCGCACACACGGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACGATCAGATCCATTAGAGNNNNNNNNAATGGTGTTCAAGTTCNATACTTCTAAGANCCAAACAAATCAAAGCAAAACGCGAGAGNTAGANGAAGATGATTATACCATAAACGAGATCCAGGACCGTACTTCTTNGGATGAGAGTTCCANACATTAGAGTGACCCNTTTNGNCGATTTGCTNACAGAGGAATNACAAACGGCGACGAGAGANACNCTTCAAAACCCTAGAAATTTAGTCNNNNTGANGAAGNNGCTGAGNTTTTATAACTNGTNCANTTTTTATCTAAANNGGCCNNNNNNNNNNAGGCCCNTTAATTGGNGGNTTNNTTTTTTTNTNTNNTTCTTCTCCATCCACATGTTCTTTGCTTTGAATGATCNTNNNNNNNTTTTTTTTTTTTTTTTTTNGAGTGAATCCATGGCCANTCGACCTCTCATCTCTCTGCAACTTCNNNNNNCTTCTTCTTCATNTTCCTCGTTAAAANCAACAACAAAACTTNTCTTCTNTCATAAACCTGAACTAATTAGCCACCACCATAGAAACCGTAACAAGTTGTCNCGNACAAGNGNTCATCAANNNTCAGCTAATCTCATCATTCANCCTTCTGTTCTTTTCCTAAGTAGCGCCTTCGATGGCGGTGGAGGATTCATCGATACACAGACTTTNATTGTCACCATTAGTCTCGTTGTAGCCATTGCTCTCTCNCTATTCCTCGGCTTCAAGGTTTNGTATCNATTATTCTTCTCTACTTAATCGTTCAATAAGTNATTTTGGTGNGNTTTTGATCTTTTTANTGTCTTATTGATTTGTTTCAGGGAGATCCAGTTCCNTGTGAGAGATGTGGTGGAAACGGTGAATCNTTTACTCTGTCTCTCTNTGTTTGCATTATGATTAGNANATGTCTTATTAANTTTTTAGTCACCTATANCANGAAATCAAAATCCATTAGGTTNTATGTAANTTATNTTNNAAAAAATTNNAAACTAGTTTTACTNTGTAGCATGNTTGTCTTTNGATTGGATACTTATGCATTGNNCAATGNAAGTAAACAATGTANACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13974188_13974343_oy-0_wil-2_79450_1652_TRUE
SEQUENCE_TARGET=950,255
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTATATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_5_10060208_10060358_tsu-0_edi-0_2106941_4760_TRUE
SEQUENCE_TARGET=950,250
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_2924839_2924934_oy-0_can-0_972477_788_TRUE
SEQUENCE_TARGET=950,195
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTNNTTCTTTCTTTNTTTAAACANATNTGAACTNTAGAACAAGCGACCGTGAGTNATAANTAAAAGGAAAGAGATCNAAANATATAAATNNAACTACTTGNAATCGACTAAATATATCGAAATCACGTGACAAATCAGCATTTTATTNNTTCTATTAACTTTATAGTTGGTTCCACATCCTTTCACTCTATTCGATGTATTGTACACATCTGGCACANNNNNNNNNNNNTGTCTTCTTCTTCGATCCTCGNTTTCATCCACANTATACAANNGNNAAATTTCCGATTNTCTCCTCACTGNNTCTCTAGAATCCATCNTNGATTCAAACNCATTTTCCGATCAAACGNCATNTTNTTCCCTNTCGAGNGAGNGATAATGTGGAAACTGAAGATCGCGGAAGGAGGTAGTCCATGGCTTAGAACCACCAATAATCACGTCGGAAGACAGTTTTGGGAGTTCGATCCGAATCTCGGTNNTCCTGANGATCTNGCCGCCGTCGANGAAGCTAGGAAGTCTTTTTCNGANAATCGATTCGTGCAGAAACATAGCGCCGATCTGCTTATGCGNCTTCAGGTATTATCTCTNNCTTCTCTTTGTTTCCNTTGACTTTAGNGTGGTNTTGACTGGTGACGAATCTCGCAAGTTTTAGTCAGTGATACTTAGGACTGTGTTGCTTCATCNNANNNNNNNNNNNNNNNTNCANCNCTNGNNNTNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGTGAACTTAGCTNGTAACTAGATTTGATTGTATCTTACTNCATGGATATGNANGAGANNNNNNNNTACAATAGATGGNAAAGTACANAGAGCATATACAACNNCAAAANCTAGAGTTAATNNANNNTNNNNNNNTTNTCNNNNNNNNTNANAANNNAATNNCNATNTNGNGNNNNTANNANNNNNNNNNGANTCNNNTANNGNTTNATTTNCANCNNNNNNNNNNNNNTNNNNNNNNNGNATTTNGTCNNAAGNTGANNNCNNAANATNTNNNNNAACNTANNNCTANNATTNTTTNANNGNTTNTNTTTACNACNNNTTGTNNNCNNTTNTNTNTCTANAGNNTNTNANNATNTGNNTTNNNTTANTNNTANNNNNNNNNNNNNNNNTNNCTTNCTNNTNNANTNTNTNNTNTTNNNNCAGNNNNTNATNTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTCTCTCTTTCTCTNTNTGTAGTTTTCAAGAGAAAATTTGATTANCCCAGTTNTACCTCAAGTCAAAATCGAAGACACTGATGATGTTACAGAGGAGNTGGTGGAAACNACGTTAAAGAGGGGTCTAGATTTCTATTCAACTATACAGGCACACGANGGGCACTGGCCAGGTGATTATGGTGGTCCTATGTTTCTTCTCCCAGGACTGGTCAGTTCATATNTTTCGTGCCTAAGCTTTCTTTTGNAAATTTTACNGAAAACTTGACAAAAACTANTTNTGATNACAGATAATTACACTCTCCATAACTGGAGCACTGAATACAGTATTGTCGGAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.793728
PRIMER_LEFT_0_PENALTY=0.148509
PRIMER_RIGHT_0_PENALTY=2.645218
PRIMER_LEFT_0_SEQUENCE=ACGTCGGAAGACAGTTTTGG
PRIMER_RIGHT_0_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_0=848,20
PRIMER_RIGHT_0=1836,22
PRIMER_LEFT_0_TM=60.149
PRIMER_RIGHT_0_TM=59.355
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=989
PRIMER_PAIR_1_PENALTY=2.869410
PRIMER_LEFT_1_PENALTY=0.224191
PRIMER_RIGHT_1_PENALTY=2.645218
PRIMER_LEFT_1_SEQUENCE=TCGGAAGACAGTTTTGGGAG
PRIMER_RIGHT_1_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_1=851,20
PRIMER_RIGHT_1=1836,22
PRIMER_LEFT_1_TM=60.224
PRIMER_RIGHT_1_TM=59.355
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=986
PRIMER_PAIR_2_PENALTY=3.249978
PRIMER_LEFT_2_PENALTY=0.604759
PRIMER_RIGHT_2_PENALTY=2.645218
PRIMER_LEFT_2_SEQUENCE=CACCAATAATCACGTCGGAA
PRIMER_RIGHT_2_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_2=837,20
PRIMER_RIGHT_2=1836,22
PRIMER_LEFT_2_TM=59.395
PRIMER_RIGHT_2_TM=59.355
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=1000
PRIMER_PAIR_3_PENALTY=3.306362
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=3.157853
PRIMER_LEFT_3_SEQUENCE=ACGTCGGAAGACAGTTTTGG
PRIMER_RIGHT_3_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_3=848,20
PRIMER_RIGHT_3=1837,23
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=60.158
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=990
PRIMER_PAIR_4_PENALTY=3.382045
PRIMER_LEFT_4_PENALTY=0.224191
PRIMER_RIGHT_4_PENALTY=3.157853
PRIMER_LEFT_4_SEQUENCE=TCGGAAGACAGTTTTGGGAG
PRIMER_RIGHT_4_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_4=851,20
PRIMER_RIGHT_4=1837,23
PRIMER_LEFT_4_TM=60.224
PRIMER_RIGHT_4_TM=60.158
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=987
PRIMER_PAIR_5_PENALTY=3.462154
PRIMER_LEFT_5_PENALTY=0.816936
PRIMER_RIGHT_5_PENALTY=2.645218
PRIMER_LEFT_5_SEQUENCE=CGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_5_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_5=852,20
PRIMER_RIGHT_5=1836,22
PRIMER_LEFT_5_TM=59.183
PRIMER_RIGHT_5_TM=59.355
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=985
PRIMER_PAIR_6_PENALTY=3.974789
PRIMER_LEFT_6_PENALTY=0.816936
PRIMER_RIGHT_6_PENALTY=3.157853
PRIMER_LEFT_6_SEQUENCE=CGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_6_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_6=852,20
PRIMER_RIGHT_6=1837,23
PRIMER_LEFT_6_TM=59.183
PRIMER_RIGHT_6_TM=60.158
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=986
PRIMER_PAIR_7_PENALTY=4.073026
PRIMER_LEFT_7_PENALTY=1.427808
PRIMER_RIGHT_7_PENALTY=2.645218
PRIMER_LEFT_7_SEQUENCE=CCAATAATCACGTCGGAAGA
PRIMER_RIGHT_7_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_7=839,20
PRIMER_RIGHT_7=1836,22
PRIMER_LEFT_7_TM=58.572
PRIMER_RIGHT_7_TM=59.355
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=998
PRIMER_PAIR_8_PENALTY=4.158975
PRIMER_LEFT_8_PENALTY=1.513757
PRIMER_RIGHT_8_PENALTY=2.645218
PRIMER_LEFT_8_SEQUENCE=CGGAAGACAGTTTTGGGAGTT
PRIMER_RIGHT_8_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_8=852,21
PRIMER_RIGHT_8=1836,22
PRIMER_LEFT_8_TM=60.514
PRIMER_RIGHT_8_TM=59.355
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=7.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=985
PRIMER_PAIR_9_PENALTY=4.201987
PRIMER_LEFT_9_PENALTY=1.556768
PRIMER_RIGHT_9_PENALTY=2.645218
PRIMER_LEFT_9_SEQUENCE=ACCAATAATCACGTCGGAAGA
PRIMER_RIGHT_9_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_9=838,21
PRIMER_RIGHT_9=1836,22
PRIMER_LEFT_9_TM=59.443
PRIMER_RIGHT_9_TM=59.355
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=999
PRIMER_PAIR_10_PENALTY=4.585661
PRIMER_LEFT_10_PENALTY=1.427808
PRIMER_RIGHT_10_PENALTY=3.157853
PRIMER_LEFT_10_SEQUENCE=CCAATAATCACGTCGGAAGA
PRIMER_RIGHT_10_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_10=839,20
PRIMER_RIGHT_10=1837,23
PRIMER_LEFT_10_TM=58.572
PRIMER_RIGHT_10_TM=60.158
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=999
PRIMER_PAIR_11_PENALTY=4.605097
PRIMER_LEFT_11_PENALTY=0.148509
PRIMER_RIGHT_11_PENALTY=4.456588
PRIMER_LEFT_11_SEQUENCE=ACGTCGGAAGACAGTTTTGG
PRIMER_RIGHT_11_SEQUENCE=CAGTGTCTTCGATTTTGACTTG
PRIMER_LEFT_11=848,20
PRIMER_RIGHT_11=1839,22
PRIMER_LEFT_11_TM=60.149
PRIMER_RIGHT_11_TM=57.543
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.8000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=7.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=992
PRIMER_PAIR_12_PENALTY=4.622477
PRIMER_LEFT_12_PENALTY=0.148509
PRIMER_RIGHT_12_PENALTY=4.473968
PRIMER_LEFT_12_SEQUENCE=ACGTCGGAAGACAGTTTTGG
PRIMER_RIGHT_12_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_12=848,20
PRIMER_RIGHT_12=1835,21
PRIMER_LEFT_12_TM=60.149
PRIMER_RIGHT_12_TM=56.526
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.8000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=988
PRIMER_PAIR_13_PENALTY=4.633753
PRIMER_LEFT_13_PENALTY=0.148509
PRIMER_RIGHT_13_PENALTY=4.485244
PRIMER_LEFT_13_SEQUENCE=ACGTCGGAAGACAGTTTTGG
PRIMER_RIGHT_13_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_13=848,20
PRIMER_RIGHT_13=1834,20
PRIMER_LEFT_13_TM=60.149
PRIMER_RIGHT_13_TM=55.515
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.8000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=987
PRIMER_PAIR_14_PENALTY=4.671610
PRIMER_LEFT_14_PENALTY=1.513757
PRIMER_RIGHT_14_PENALTY=3.157853
PRIMER_LEFT_14_SEQUENCE=CGGAAGACAGTTTTGGGAGTT
PRIMER_RIGHT_14_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_14=852,21
PRIMER_RIGHT_14=1837,23
PRIMER_LEFT_14_TM=60.514
PRIMER_RIGHT_14_TM=60.158
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=986
PRIMER_PAIR_15_PENALTY=4.676763
PRIMER_LEFT_15_PENALTY=2.031545
PRIMER_RIGHT_15_PENALTY=2.645218
PRIMER_LEFT_15_SEQUENCE=TCGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_15_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_15=851,21
PRIMER_RIGHT_15=1836,22
PRIMER_LEFT_15_TM=61.032
PRIMER_RIGHT_15_TM=59.355
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=986
PRIMER_PAIR_16_PENALTY=4.680779
PRIMER_LEFT_16_PENALTY=0.224191
PRIMER_RIGHT_16_PENALTY=4.456588
PRIMER_LEFT_16_SEQUENCE=TCGGAAGACAGTTTTGGGAG
PRIMER_RIGHT_16_SEQUENCE=CAGTGTCTTCGATTTTGACTTG
PRIMER_LEFT_16=851,20
PRIMER_RIGHT_16=1839,22
PRIMER_LEFT_16_TM=60.224
PRIMER_RIGHT_16_TM=57.543
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=989
PRIMER_PAIR_17_PENALTY=4.698159
PRIMER_LEFT_17_PENALTY=0.224191
PRIMER_RIGHT_17_PENALTY=4.473968
PRIMER_LEFT_17_SEQUENCE=TCGGAAGACAGTTTTGGGAG
PRIMER_RIGHT_17_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_17=851,20
PRIMER_RIGHT_17=1835,21
PRIMER_LEFT_17_TM=60.224
PRIMER_RIGHT_17_TM=56.526
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=985
PRIMER_PAIR_18_PENALTY=4.709435
PRIMER_LEFT_18_PENALTY=0.224191
PRIMER_RIGHT_18_PENALTY=4.485244
PRIMER_LEFT_18_SEQUENCE=TCGGAAGACAGTTTTGGGAG
PRIMER_RIGHT_18_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_18=851,20
PRIMER_RIGHT_18=1834,20
PRIMER_LEFT_18_TM=60.224
PRIMER_RIGHT_18_TM=55.515
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=984
PRIMER_PAIR_19_PENALTY=4.714622
PRIMER_LEFT_19_PENALTY=1.556768
PRIMER_RIGHT_19_PENALTY=3.157853
PRIMER_LEFT_19_SEQUENCE=ACCAATAATCACGTCGGAAGA
PRIMER_RIGHT_19_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_19=838,21
PRIMER_RIGHT_19=1837,23
PRIMER_LEFT_19_TM=59.443
PRIMER_RIGHT_19_TM=60.158
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=1000
PRIMER_PAIR_20_PENALTY=4.896593
PRIMER_LEFT_20_PENALTY=0.224191
PRIMER_RIGHT_20_PENALTY=4.672402
PRIMER_LEFT_20_SEQUENCE=TCGGAAGACAGTTTTGGGAG
PRIMER_RIGHT_20_SEQUENCE=CATCAGTGTCTTCGATTTTGAC
PRIMER_LEFT_20=851,20
PRIMER_RIGHT_20=1842,22
PRIMER_LEFT_20_TM=60.224
PRIMER_RIGHT_20_TM=57.328
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=7.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=992
PRIMER_PAIR_21_PENALTY=5.078727
PRIMER_LEFT_21_PENALTY=0.604759
PRIMER_RIGHT_21_PENALTY=4.473968
PRIMER_LEFT_21_SEQUENCE=CACCAATAATCACGTCGGAA
PRIMER_RIGHT_21_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_21=837,20
PRIMER_RIGHT_21=1835,21
PRIMER_LEFT_21_TM=59.395
PRIMER_RIGHT_21_TM=56.526
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=10.2000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=999
PRIMER_PAIR_22_PENALTY=5.090003
PRIMER_LEFT_22_PENALTY=0.604759
PRIMER_RIGHT_22_PENALTY=4.485244
PRIMER_LEFT_22_SEQUENCE=CACCAATAATCACGTCGGAA
PRIMER_RIGHT_22_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_22=837,20
PRIMER_RIGHT_22=1834,20
PRIMER_LEFT_22_TM=59.395
PRIMER_RIGHT_22_TM=55.515
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=10.2000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=998
PRIMER_PAIR_23_PENALTY=5.121276
PRIMER_LEFT_23_PENALTY=0.636033
PRIMER_RIGHT_23_PENALTY=4.485244
PRIMER_LEFT_23_SEQUENCE=ACCACCAATAATCACGTCGG
PRIMER_RIGHT_23_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_23=835,20
PRIMER_RIGHT_23=1834,20
PRIMER_LEFT_23_TM=60.636
PRIMER_RIGHT_23_TM=55.515
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.6000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=1000
PRIMER_PAIR_24_PENALTY=5.165410
PRIMER_LEFT_24_PENALTY=0.148509
PRIMER_RIGHT_24_PENALTY=5.016901
PRIMER_LEFT_24_SEQUENCE=ACGTCGGAAGACAGTTTTGG
PRIMER_RIGHT_24_SEQUENCE=AGTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_24=848,20
PRIMER_RIGHT_24=1838,24
PRIMER_LEFT_24_TM=60.149
PRIMER_RIGHT_24_TM=61.017
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=41.667
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=7.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=991
PRIMER_PAIR_25_PENALTY=5.189398
PRIMER_LEFT_25_PENALTY=2.031545
PRIMER_RIGHT_25_PENALTY=3.157853
PRIMER_LEFT_25_SEQUENCE=TCGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_25_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_25=851,21
PRIMER_RIGHT_25=1837,23
PRIMER_LEFT_25_TM=61.032
PRIMER_RIGHT_25_TM=60.158
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=7.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=987
PRIMER_PAIR_26_PENALTY=5.241092
PRIMER_LEFT_26_PENALTY=0.224191
PRIMER_RIGHT_26_PENALTY=5.016901
PRIMER_LEFT_26_SEQUENCE=TCGGAAGACAGTTTTGGGAG
PRIMER_RIGHT_26_SEQUENCE=AGTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_26=851,20
PRIMER_RIGHT_26=1838,24
PRIMER_LEFT_26_TM=60.224
PRIMER_RIGHT_26_TM=61.017
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=41.667
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=988
PRIMER_PAIR_27_PENALTY=5.273524
PRIMER_LEFT_27_PENALTY=0.816936
PRIMER_RIGHT_27_PENALTY=4.456588
PRIMER_LEFT_27_SEQUENCE=CGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_27_SEQUENCE=CAGTGTCTTCGATTTTGACTTG
PRIMER_LEFT_27=852,20
PRIMER_RIGHT_27=1839,22
PRIMER_LEFT_27_TM=59.183
PRIMER_RIGHT_27_TM=57.543
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=988
PRIMER_PAIR_28_PENALTY=5.290904
PRIMER_LEFT_28_PENALTY=0.816936
PRIMER_RIGHT_28_PENALTY=4.473968
PRIMER_LEFT_28_SEQUENCE=CGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_28_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_28=852,20
PRIMER_RIGHT_28=1835,21
PRIMER_LEFT_28_TM=59.183
PRIMER_RIGHT_28_TM=56.526
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=984
PRIMER_PAIR_29_PENALTY=5.302180
PRIMER_LEFT_29_PENALTY=0.816936
PRIMER_RIGHT_29_PENALTY=4.485244
PRIMER_LEFT_29_SEQUENCE=CGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_29_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_29=852,20
PRIMER_RIGHT_29=1834,20
PRIMER_LEFT_29_TM=59.183
PRIMER_RIGHT_29_TM=55.515
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=983
PRIMER_PAIR_30_PENALTY=5.420691
PRIMER_LEFT_30_PENALTY=2.775473
PRIMER_RIGHT_30_PENALTY=2.645218
PRIMER_LEFT_30_SEQUENCE=AATCACGTCGGAAGACAGTT
PRIMER_RIGHT_30_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_30=844,20
PRIMER_RIGHT_30=1836,22
PRIMER_LEFT_30_TM=57.225
PRIMER_RIGHT_30_TM=59.355
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=7.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=993
PRIMER_PAIR_31_PENALTY=5.489338
PRIMER_LEFT_31_PENALTY=0.816936
PRIMER_RIGHT_31_PENALTY=4.672402
PRIMER_LEFT_31_SEQUENCE=CGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_31_SEQUENCE=CATCAGTGTCTTCGATTTTGAC
PRIMER_LEFT_31=852,20
PRIMER_RIGHT_31=1842,22
PRIMER_LEFT_31_TM=59.183
PRIMER_RIGHT_31_TM=57.328
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=7.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=991
PRIMER_PAIR_32_PENALTY=5.526043
PRIMER_LEFT_32_PENALTY=2.368190
PRIMER_RIGHT_32_PENALTY=3.157853
PRIMER_LEFT_32_SEQUENCE=CAATAATCACGTCGGAAGACAG
PRIMER_RIGHT_32_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_32=840,22
PRIMER_RIGHT_32=1837,23
PRIMER_LEFT_32_TM=59.632
PRIMER_RIGHT_32_TM=60.158
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=43.478
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=998
PRIMER_PAIR_33_PENALTY=5.833837
PRIMER_LEFT_33_PENALTY=0.816936
PRIMER_RIGHT_33_PENALTY=5.016901
PRIMER_LEFT_33_SEQUENCE=CGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_33_SEQUENCE=AGTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_33=852,20
PRIMER_RIGHT_33=1838,24
PRIMER_LEFT_33_TM=59.183
PRIMER_RIGHT_33_TM=61.017
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=41.667
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.6000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=987
PRIMER_PAIR_34_PENALTY=5.901775
PRIMER_LEFT_34_PENALTY=1.427808
PRIMER_RIGHT_34_PENALTY=4.473968
PRIMER_LEFT_34_SEQUENCE=CCAATAATCACGTCGGAAGA
PRIMER_RIGHT_34_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_34=839,20
PRIMER_RIGHT_34=1835,21
PRIMER_LEFT_34_TM=58.572
PRIMER_RIGHT_34_TM=56.526
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=997
PRIMER_PAIR_35_PENALTY=5.913051
PRIMER_LEFT_35_PENALTY=1.427808
PRIMER_RIGHT_35_PENALTY=4.485244
PRIMER_LEFT_35_SEQUENCE=CCAATAATCACGTCGGAAGA
PRIMER_RIGHT_35_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_35=839,20
PRIMER_RIGHT_35=1834,20
PRIMER_LEFT_35_TM=58.572
PRIMER_RIGHT_35_TM=55.515
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=996
PRIMER_PAIR_36_PENALTY=5.933326
PRIMER_LEFT_36_PENALTY=2.775473
PRIMER_RIGHT_36_PENALTY=3.157853
PRIMER_LEFT_36_SEQUENCE=AATCACGTCGGAAGACAGTT
PRIMER_RIGHT_36_SEQUENCE=GTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_36=844,20
PRIMER_RIGHT_36=1837,23
PRIMER_LEFT_36_TM=57.225
PRIMER_RIGHT_36_TM=60.158
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=7.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=994
PRIMER_PAIR_37_PENALTY=5.970344
PRIMER_LEFT_37_PENALTY=1.513757
PRIMER_RIGHT_37_PENALTY=4.456588
PRIMER_LEFT_37_SEQUENCE=CGGAAGACAGTTTTGGGAGTT
PRIMER_RIGHT_37_SEQUENCE=CAGTGTCTTCGATTTTGACTTG
PRIMER_LEFT_37=852,21
PRIMER_RIGHT_37=1839,22
PRIMER_LEFT_37_TM=60.514
PRIMER_RIGHT_37_TM=57.543
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=7.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=988
PRIMER_PAIR_38_PENALTY=5.987724
PRIMER_LEFT_38_PENALTY=1.513757
PRIMER_RIGHT_38_PENALTY=4.473968
PRIMER_LEFT_38_SEQUENCE=CGGAAGACAGTTTTGGGAGTT
PRIMER_RIGHT_38_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_38=852,21
PRIMER_RIGHT_38=1835,21
PRIMER_LEFT_38_TM=60.514
PRIMER_RIGHT_38_TM=56.526
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=984
PRIMER_PAIR_39_PENALTY=5.999000
PRIMER_LEFT_39_PENALTY=1.513757
PRIMER_RIGHT_39_PENALTY=4.485244
PRIMER_LEFT_39_SEQUENCE=CGGAAGACAGTTTTGGGAGTT
PRIMER_RIGHT_39_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_39=852,21
PRIMER_RIGHT_39=1834,20
PRIMER_LEFT_39_TM=60.514
PRIMER_RIGHT_39_TM=55.515
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=983
PRIMER_PAIR_40_PENALTY=6.030736
PRIMER_LEFT_40_PENALTY=1.556768
PRIMER_RIGHT_40_PENALTY=4.473968
PRIMER_LEFT_40_SEQUENCE=ACCAATAATCACGTCGGAAGA
PRIMER_RIGHT_40_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_40=838,21
PRIMER_RIGHT_40=1835,21
PRIMER_LEFT_40_TM=59.443
PRIMER_RIGHT_40_TM=56.526
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=998
PRIMER_PAIR_41_PENALTY=6.042012
PRIMER_LEFT_41_PENALTY=1.556768
PRIMER_RIGHT_41_PENALTY=4.485244
PRIMER_LEFT_41_SEQUENCE=ACCAATAATCACGTCGGAAGA
PRIMER_RIGHT_41_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_41=838,21
PRIMER_RIGHT_41=1834,20
PRIMER_LEFT_41_TM=59.443
PRIMER_RIGHT_41_TM=55.515
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=997
PRIMER_PAIR_42_PENALTY=6.186159
PRIMER_LEFT_42_PENALTY=1.513757
PRIMER_RIGHT_42_PENALTY=4.672402
PRIMER_LEFT_42_SEQUENCE=CGGAAGACAGTTTTGGGAGTT
PRIMER_RIGHT_42_SEQUENCE=CATCAGTGTCTTCGATTTTGAC
PRIMER_LEFT_42=852,21
PRIMER_RIGHT_42=1842,22
PRIMER_LEFT_42_TM=60.514
PRIMER_RIGHT_42_TM=57.328
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=991
PRIMER_PAIR_43_PENALTY=6.301217
PRIMER_LEFT_43_PENALTY=3.655999
PRIMER_RIGHT_43_PENALTY=2.645218
PRIMER_LEFT_43_SEQUENCE=TAATCACGTCGGAAGACAGTT
PRIMER_RIGHT_43_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_43=843,21
PRIMER_RIGHT_43=1836,22
PRIMER_LEFT_43_TM=57.344
PRIMER_RIGHT_43_TM=59.355
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=7.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=994
PRIMER_PAIR_44_PENALTY=6.423648
PRIMER_LEFT_44_PENALTY=3.778429
PRIMER_RIGHT_44_PENALTY=2.645218
PRIMER_LEFT_44_SEQUENCE=GTCGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_44_SEQUENCE=TGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_44=850,22
PRIMER_RIGHT_44=1836,22
PRIMER_LEFT_44_TM=61.778
PRIMER_RIGHT_44_TM=59.355
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=7.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=987
PRIMER_PAIR_45_PENALTY=6.444709
PRIMER_LEFT_45_PENALTY=1.427808
PRIMER_RIGHT_45_PENALTY=5.016901
PRIMER_LEFT_45_SEQUENCE=CCAATAATCACGTCGGAAGA
PRIMER_RIGHT_45_SEQUENCE=AGTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_45=839,20
PRIMER_RIGHT_45=1838,24
PRIMER_LEFT_45_TM=58.572
PRIMER_RIGHT_45_TM=61.017
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=41.667
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=1000
PRIMER_PAIR_46_PENALTY=6.488133
PRIMER_LEFT_46_PENALTY=2.031545
PRIMER_RIGHT_46_PENALTY=4.456588
PRIMER_LEFT_46_SEQUENCE=TCGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_46_SEQUENCE=CAGTGTCTTCGATTTTGACTTG
PRIMER_LEFT_46=851,21
PRIMER_RIGHT_46=1839,22
PRIMER_LEFT_46_TM=61.032
PRIMER_RIGHT_46_TM=57.543
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.6000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=989
PRIMER_PAIR_47_PENALTY=6.505513
PRIMER_LEFT_47_PENALTY=2.031545
PRIMER_RIGHT_47_PENALTY=4.473968
PRIMER_LEFT_47_SEQUENCE=TCGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_47_SEQUENCE=GTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_47=851,21
PRIMER_RIGHT_47=1835,21
PRIMER_LEFT_47_TM=61.032
PRIMER_RIGHT_47_TM=56.526
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=985
PRIMER_PAIR_48_PENALTY=6.516789
PRIMER_LEFT_48_PENALTY=2.031545
PRIMER_RIGHT_48_PENALTY=4.485244
PRIMER_LEFT_48_SEQUENCE=TCGGAAGACAGTTTTGGGAGT
PRIMER_RIGHT_48_SEQUENCE=TCTTCGATTTTGACTTGAGG
PRIMER_LEFT_48=851,21
PRIMER_RIGHT_48=1834,20
PRIMER_LEFT_48_TM=61.032
PRIMER_RIGHT_48_TM=55.515
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=984
PRIMER_PAIR_49_PENALTY=6.530658
PRIMER_LEFT_49_PENALTY=1.513757
PRIMER_RIGHT_49_PENALTY=5.016901
PRIMER_LEFT_49_SEQUENCE=CGGAAGACAGTTTTGGGAGTT
PRIMER_RIGHT_49_SEQUENCE=AGTGTCTTCGATTTTGACTTGAGG
PRIMER_LEFT_49=852,21
PRIMER_RIGHT_49=1838,24
PRIMER_LEFT_49_TM=60.514
PRIMER_RIGHT_49_TM=61.017
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=41.667
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=987
=
SEQUENCE_ID=MAGIC.329_1_15507551_15507663_edi-0_ler-0_425730_137606_TRUE
SEQUENCE_TARGET=950,212
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_5365611_5365832_can-0_ws-0_209685_41414_TRUE
SEQUENCE_TARGET=950,321
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_1170619_1170627_ler-0_zu-0_38873_69004_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=TACAAAGATTAGTCAAGAAAATTCACCAATCTTCTTCTATCTCAAGAACATGATAACAATAGATGAGACCAAGAACACCGTTTAGTACTTTTCCAAATTTGTAGANAAGTTATGTCTCTCTTGTTTTGTAACCNTGTGACTNNNNNNNGTGAGTACTCTTGTGATATAGTNAGTGGTTATAGAAGAANAAGTTGTATGAATGTCATGTCTATCTTNTTTTGTAAANTTTCCCTATTGTATGTNTGNCCCAATATATAAAACCGGTGAATTTGTGATATAATGAGAAGTGGTTCNAGAAGACAAAGTTGGATAATTTCATTTCATACACTATACAACTAATAAAATGGTCAAGTTTTCATTGACATANCAATCAACATCGAGTCTTTTTAAGTCCTACCTAATACCCCCAAAAGATTTCAGGTTCTNAACAAGAGAAAGCAAGGATAATATCAAATGAAGATGCTTCTATTTTAGNCNTNNNNNNNNNNNNTTACGTGCAGCNTGTCACTTTTATGTTTTAAGTCCAAACCNCATTANANTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTCACTTTATCGAAGAAAAAAANNTTTNCNAGANTATCTTCAATTCNAAATCTGCAATTAATCTCCAAATCCACTCAACNCGATNNCTTTCTTTAAGATTGTTAATGGGTAAATAAGTAAAAGTTNTAATAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCNCCGCAATTTTAAATGGAGAAGNCANTTTAAATAAGCAAAATAAANNACAATCTTAAAGAAACCAAGACCTCNTATTCTCCAATTANTNAAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTANACTAATGCTAACNTTTTNTTTNNTTNTTCATTTTGATANNTTTTTATTTTATAATTTATAAACAAATACTTGAACAAGATTCTTTGAGTAAAATACTTTAGCCAATCTAAATCAAAAATATTTCAGCAAAACTGTTGTTNCCAANCATTTCTCTAAAATCGATACCAAAAATCAAANGANNNAAAAAAAAAAAACATTTTGTAAATTGAAAGTTGAAACTGTGCCTTGGTTGAAAAGTAAGAACGNNGCGTGTAGTTANACGCTTCNACNTGGCCCAACNNGTTCACATATTTTAGATTTGGTCGTTGTTGGTGTTTGGTAACATGTCCTGGCCCACCACCAANTAATTCTATAATTGNNNNNNTGNNTCTTATCTNNCTATCANNATCCTAAATTGCAACTTGCTGACGCTTTACCGGNCCAACGTAACTTTACTACACCTTGGTAACGTGTCTAATAAGCTGTATTTAGCAAAATTTTACCATGTAATATGTGGTTTACTTTTTTGGCATCATTTGTTT
PRIMER_LEFT_NUM_RETURNED=34
PRIMER_RIGHT_NUM_RETURNED=34
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=34
PRIMER_PAIR_0_PENALTY=7.510173
PRIMER_LEFT_0_PENALTY=7.232188
PRIMER_RIGHT_0_PENALTY=0.277985
PRIMER_LEFT_0_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_0_SEQUENCE=TCAACCAAGGCACAGTTTCA
PRIMER_LEFT_0=749,25
PRIMER_RIGHT_0=1719,20
PRIMER_LEFT_0_TM=62.232
PRIMER_RIGHT_0_TM=60.278
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=971
PRIMER_PAIR_1_PENALTY=8.371604
PRIMER_LEFT_1_PENALTY=7.232188
PRIMER_RIGHT_1_PENALTY=1.139416
PRIMER_LEFT_1_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_1_SEQUENCE=TTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_1=749,25
PRIMER_RIGHT_1=1721,21
PRIMER_LEFT_1_TM=62.232
PRIMER_RIGHT_1_TM=60.139
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=8.483659
PRIMER_LEFT_2_PENALTY=7.232188
PRIMER_RIGHT_2_PENALTY=1.251471
PRIMER_LEFT_2_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_2_SEQUENCE=TTCAACCAAGGCACAGTTTC
PRIMER_LEFT_2=749,25
PRIMER_RIGHT_2=1720,20
PRIMER_LEFT_2_TM=62.232
PRIMER_RIGHT_2_TM=58.749
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=972
PRIMER_PAIR_3_PENALTY=8.960433
PRIMER_LEFT_3_PENALTY=7.232188
PRIMER_RIGHT_3_PENALTY=1.728245
PRIMER_LEFT_3_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_3_SEQUENCE=CTTTTCAACCAAGGCACAGTT
PRIMER_LEFT_3=749,25
PRIMER_RIGHT_3=1723,21
PRIMER_LEFT_3_TM=62.232
PRIMER_RIGHT_3_TM=59.272
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=975
PRIMER_PAIR_4_PENALTY=8.976615
PRIMER_LEFT_4_PENALTY=7.232188
PRIMER_RIGHT_4_PENALTY=1.744427
PRIMER_LEFT_4_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_4_SEQUENCE=TTTCAACCAAGGCACAGTTT
PRIMER_LEFT_4=749,25
PRIMER_RIGHT_4=1721,20
PRIMER_LEFT_4_TM=62.232
PRIMER_RIGHT_4_TM=58.256
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=973
PRIMER_PAIR_5_PENALTY=8.976615
PRIMER_LEFT_5_PENALTY=7.232188
PRIMER_RIGHT_5_PENALTY=1.744427
PRIMER_LEFT_5_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_5_SEQUENCE=TTTTCAACCAAGGCACAGTT
PRIMER_LEFT_5=749,25
PRIMER_RIGHT_5=1722,20
PRIMER_LEFT_5_TM=62.232
PRIMER_RIGHT_5_TM=58.256
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=974
PRIMER_PAIR_6_PENALTY=9.306996
PRIMER_LEFT_6_PENALTY=7.232188
PRIMER_RIGHT_6_PENALTY=2.074807
PRIMER_LEFT_6_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_6_SEQUENCE=ACTTTTCAACCAAGGCACAGTT
PRIMER_LEFT_6=749,25
PRIMER_RIGHT_6=1724,22
PRIMER_LEFT_6_TM=62.232
PRIMER_RIGHT_6_TM=60.075
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=976
PRIMER_PAIR_7_PENALTY=9.376352
PRIMER_LEFT_7_PENALTY=7.232188
PRIMER_RIGHT_7_PENALTY=2.144164
PRIMER_LEFT_7_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_7_SEQUENCE=ACTTTTCAACCAAGGCACAG
PRIMER_LEFT_7=749,25
PRIMER_RIGHT_7=1724,20
PRIMER_LEFT_7_TM=62.232
PRIMER_RIGHT_7_TM=57.856
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=976
PRIMER_PAIR_8_PENALTY=9.776557
PRIMER_LEFT_8_PENALTY=7.232188
PRIMER_RIGHT_8_PENALTY=2.544368
PRIMER_LEFT_8_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_8_SEQUENCE=CTTTTCAACCAAGGCACAGTTT
PRIMER_LEFT_8=749,25
PRIMER_RIGHT_8=1723,22
PRIMER_LEFT_8_TM=62.232
PRIMER_RIGHT_8_TM=60.544
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=975
PRIMER_PAIR_9_PENALTY=9.851812
PRIMER_LEFT_9_PENALTY=7.232188
PRIMER_RIGHT_9_PENALTY=2.619623
PRIMER_LEFT_9_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_9_SEQUENCE=TTCAACCAAGGCACAGTTTCA
PRIMER_LEFT_9=749,25
PRIMER_RIGHT_9=1720,21
PRIMER_LEFT_9_TM=62.232
PRIMER_RIGHT_9_TM=61.620
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=972
PRIMER_PAIR_10_PENALTY=9.923869
PRIMER_LEFT_10_PENALTY=7.232188
PRIMER_RIGHT_10_PENALTY=2.691680
PRIMER_LEFT_10_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_10_SEQUENCE=TTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_10=749,25
PRIMER_RIGHT_10=1726,22
PRIMER_LEFT_10_TM=62.232
PRIMER_RIGHT_10_TM=59.308
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=978
PRIMER_PAIR_11_PENALTY=9.923869
PRIMER_LEFT_11_PENALTY=7.232188
PRIMER_RIGHT_11_PENALTY=2.691680
PRIMER_LEFT_11_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_11_SEQUENCE=CTTACTTTTCAACCAAGGCACA
PRIMER_LEFT_11=749,25
PRIMER_RIGHT_11=1727,22
PRIMER_LEFT_11_TM=62.232
PRIMER_RIGHT_11_TM=59.308
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=979
PRIMER_PAIR_12_PENALTY=10.283811
PRIMER_LEFT_12_PENALTY=7.232188
PRIMER_RIGHT_12_PENALTY=3.051623
PRIMER_LEFT_12_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_12_SEQUENCE=TACTTTTCAACCAAGGCACAG
PRIMER_LEFT_12=749,25
PRIMER_RIGHT_12=1725,21
PRIMER_LEFT_12_TM=62.232
PRIMER_RIGHT_12_TM=57.948
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=977
PRIMER_PAIR_13_PENALTY=10.382248
PRIMER_LEFT_13_PENALTY=7.232188
PRIMER_RIGHT_13_PENALTY=3.150060
PRIMER_LEFT_13_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_13_SEQUENCE=TACTTTTCAACCAAGGCACA
PRIMER_LEFT_13=749,25
PRIMER_RIGHT_13=1725,20
PRIMER_LEFT_13_TM=62.232
PRIMER_RIGHT_13_TM=56.850
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=977
PRIMER_PAIR_14_PENALTY=10.433304
PRIMER_LEFT_14_PENALTY=7.232188
PRIMER_RIGHT_14_PENALTY=3.201115
PRIMER_LEFT_14_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_14_SEQUENCE=CTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_14=749,25
PRIMER_RIGHT_14=1727,23
PRIMER_LEFT_14_TM=62.232
PRIMER_RIGHT_14_TM=60.201
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=979
PRIMER_PAIR_15_PENALTY=10.619517
PRIMER_LEFT_15_PENALTY=7.232188
PRIMER_RIGHT_15_PENALTY=3.387329
PRIMER_LEFT_15_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_15_SEQUENCE=TTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_15=749,25
PRIMER_RIGHT_15=1722,22
PRIMER_LEFT_15_TM=62.232
PRIMER_RIGHT_15_TM=61.387
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=974
PRIMER_PAIR_16_PENALTY=10.805592
PRIMER_LEFT_16_PENALTY=7.232188
PRIMER_RIGHT_16_PENALTY=3.573403
PRIMER_LEFT_16_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_16_SEQUENCE=TCTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_16=749,25
PRIMER_RIGHT_16=1728,22
PRIMER_LEFT_16_TM=62.232
PRIMER_RIGHT_16_TM=58.427
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=980
PRIMER_PAIR_17_PENALTY=11.602768
PRIMER_LEFT_17_PENALTY=7.232188
PRIMER_RIGHT_17_PENALTY=4.370580
PRIMER_LEFT_17_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_17_SEQUENCE=CTTACTTTTCAACCAAGGCA
PRIMER_LEFT_17=749,25
PRIMER_RIGHT_17=1727,20
PRIMER_LEFT_17_TM=62.232
PRIMER_RIGHT_17_TM=55.629
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=979
PRIMER_PAIR_18_PENALTY=11.637489
PRIMER_LEFT_18_PENALTY=7.232188
PRIMER_RIGHT_18_PENALTY=4.405301
PRIMER_LEFT_18_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_18_SEQUENCE=CTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_18=749,25
PRIMER_RIGHT_18=1727,21
PRIMER_LEFT_18_TM=62.232
PRIMER_RIGHT_18_TM=56.595
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=979
PRIMER_PAIR_19_PENALTY=11.650553
PRIMER_LEFT_19_PENALTY=7.232188
PRIMER_RIGHT_19_PENALTY=4.418365
PRIMER_LEFT_19_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_19_SEQUENCE=GTTCTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_19=749,25
PRIMER_RIGHT_19=1730,24
PRIMER_LEFT_19_TM=62.232
PRIMER_RIGHT_19_TM=60.418
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=41.667
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=982
PRIMER_PAIR_20_PENALTY=11.772755
PRIMER_LEFT_20_PENALTY=7.232188
PRIMER_RIGHT_20_PENALTY=4.540567
PRIMER_LEFT_20_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_20_SEQUENCE=TTACTTTTCAACCAAGGCAC
PRIMER_LEFT_20=749,25
PRIMER_RIGHT_20=1726,20
PRIMER_LEFT_20_TM=62.232
PRIMER_RIGHT_20_TM=55.459
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=978
PRIMER_PAIR_21_PENALTY=12.052181
PRIMER_LEFT_21_PENALTY=7.232188
PRIMER_RIGHT_21_PENALTY=4.819993
PRIMER_LEFT_21_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_21_SEQUENCE=TTTCAACCAAGGCACAGTTTCA
PRIMER_LEFT_21=749,25
PRIMER_RIGHT_21=1721,22
PRIMER_LEFT_21_TM=62.232
PRIMER_RIGHT_21_TM=62.820
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=973
PRIMER_PAIR_22_PENALTY=12.424022
PRIMER_LEFT_22_PENALTY=7.232188
PRIMER_RIGHT_22_PENALTY=5.191833
PRIMER_LEFT_22_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_22_SEQUENCE=CTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_22=749,25
PRIMER_RIGHT_22=1723,23
PRIMER_LEFT_22_TM=62.232
PRIMER_RIGHT_22_TM=62.192
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=43.478
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=975
PRIMER_PAIR_23_PENALTY=13.045001
PRIMER_LEFT_23_PENALTY=7.232188
PRIMER_RIGHT_23_PENALTY=5.812812
PRIMER_LEFT_23_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_23_SEQUENCE=TCTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_23=749,25
PRIMER_RIGHT_23=1728,24
PRIMER_LEFT_23_TM=62.232
PRIMER_RIGHT_23_TM=61.813
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=41.667
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=980
PRIMER_PAIR_24_PENALTY=14.067425
PRIMER_LEFT_24_PENALTY=7.232188
PRIMER_RIGHT_24_PENALTY=6.835237
PRIMER_LEFT_24_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_24_SEQUENCE=ACTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_24=749,25
PRIMER_RIGHT_24=1724,24
PRIMER_LEFT_24_TM=62.232
PRIMER_RIGHT_24_TM=62.835
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=41.667
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=976
PRIMER_PAIR_25_PENALTY=14.210580
PRIMER_LEFT_25_PENALTY=7.232188
PRIMER_RIGHT_25_PENALTY=6.978392
PRIMER_LEFT_25_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_25_SEQUENCE=CTTACTTTTCAACCAAGGCACAGTT
PRIMER_LEFT_25=749,25
PRIMER_RIGHT_25=1727,25
PRIMER_LEFT_25_TM=62.232
PRIMER_RIGHT_25_TM=61.978
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=979
PRIMER_PAIR_26_PENALTY=14.866289
PRIMER_LEFT_26_PENALTY=7.232188
PRIMER_RIGHT_26_PENALTY=7.634100
PRIMER_LEFT_26_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_26_SEQUENCE=CGTTCTTACTTTTCAACCAAGGCA
PRIMER_LEFT_26=749,25
PRIMER_RIGHT_26=1731,24
PRIMER_LEFT_26_TM=62.232
PRIMER_RIGHT_26_TM=63.634
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=41.667
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=983
PRIMER_PAIR_27_PENALTY=14.984772
PRIMER_LEFT_27_PENALTY=7.232188
PRIMER_RIGHT_27_PENALTY=7.752584
PRIMER_LEFT_27_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_27_SEQUENCE=TACTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_27=749,25
PRIMER_RIGHT_27=1725,25
PRIMER_LEFT_27_TM=62.232
PRIMER_RIGHT_27_TM=62.753
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=977
PRIMER_PAIR_28_PENALTY=14.984772
PRIMER_LEFT_28_PENALTY=7.232188
PRIMER_RIGHT_28_PENALTY=7.752584
PRIMER_LEFT_28_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_28_SEQUENCE=GTTCTTACTTTTCAACCAAGGCACA
PRIMER_LEFT_28=749,25
PRIMER_RIGHT_28=1730,25
PRIMER_LEFT_28_TM=62.232
PRIMER_RIGHT_28_TM=62.753
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=982
PRIMER_PAIR_29_PENALTY=15.077551
PRIMER_LEFT_29_PENALTY=7.232188
PRIMER_RIGHT_29_PENALTY=7.845362
PRIMER_LEFT_29_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_29_SEQUENCE=TTCTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_29=749,25
PRIMER_RIGHT_29=1729,25
PRIMER_LEFT_29_TM=62.232
PRIMER_RIGHT_29_TM=62.845
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=981
PRIMER_PAIR_30_PENALTY=15.811853
PRIMER_LEFT_30_PENALTY=7.232188
PRIMER_RIGHT_30_PENALTY=8.579664
PRIMER_LEFT_30_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_30_SEQUENCE=CTTTTCAACCAAGGCACAGTTTCA
PRIMER_LEFT_30=749,25
PRIMER_RIGHT_30=1723,24
PRIMER_LEFT_30_TM=62.232
PRIMER_RIGHT_30_TM=64.580
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=41.667
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.3000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=975
PRIMER_PAIR_31_PENALTY=16.425105
PRIMER_LEFT_31_PENALTY=7.232188
PRIMER_RIGHT_31_PENALTY=9.192916
PRIMER_LEFT_31_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_31_SEQUENCE=CGTTCTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_31=749,25
PRIMER_RIGHT_31=1731,25
PRIMER_LEFT_31_TM=62.232
PRIMER_RIGHT_31_TM=64.193
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=44.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=983
PRIMER_PAIR_32_PENALTY=16.655433
PRIMER_LEFT_32_PENALTY=7.232188
PRIMER_RIGHT_32_PENALTY=9.423244
PRIMER_LEFT_32_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_32_SEQUENCE=GTTCTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_32=749,25
PRIMER_RIGHT_32=1730,26
PRIMER_LEFT_32_TM=62.232
PRIMER_RIGHT_32_TM=63.423
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=42.308
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=982
PRIMER_PAIR_33_PENALTY=18.544545
PRIMER_LEFT_33_PENALTY=7.232188
PRIMER_RIGHT_33_PENALTY=11.312356
PRIMER_LEFT_33_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_33_SEQUENCE=CTTACTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_33=749,25
PRIMER_RIGHT_33=1727,27
PRIMER_LEFT_33_TM=62.232
PRIMER_RIGHT_33_TM=64.312
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=40.741
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=979
=
SEQUENCE_ID=MAGIC.105_4_10392491_10392516_wil-2_po-0_544_6285_TRUE
SEQUENCE_TARGET=950,125
SEQUENCE_TEMPLATE=GGCAAGAGGAAACACTATGTCATGAAGATGAACATCATGATTGACATAATGTGGTAGTTGGAAGTTCATAAATTCTGTGTCTTTTTGTTCATGCATCTTCTTCTTCTTCAGTGACACAGTAAGAAAATAATCACTTTGGGAATAAATTGTGAAGCATTTGTCCACAAAGATTCNACCTTTCTGTACTAAGTAGCCACCCAAAGGATATATAAGACGATTTTAAAATAGATTTGGTAAGTAATGATGTANAGTGGACCAGTAGAAAAACTTATAAATGAANTTGTTGTTATAATGTGACNNNNNNNTTTNGAGTCAGTGACTCATAAANTTGAAGCATGATCTTGTGGGGTTTTGTGACAGAGCAAATAAGTTAAAGCAGCCATGCTGGATTCTTTTTNAAATANANAAACATTTCANAAAANAGAACAGNNAAAATGATCATATCTACTACACTACNAACCACATGCAAGGAATTATGAACACTATAAAGCATGAGTGTCATTGAACANTTGACAATTCGTTTGTTTTCTCGAGAATANAAGATAACTTTATAAATGNAGATTCNTTTTACCATCTTCTTGATGAATTCACANATTAGAAATTACAATCAAGNGTAAAAAACTTGGGGGNAACTTCTACANATACATGTGTGTGGTNNTGTGTGTTNATNAGAATGAAATCAACTATCTTCAGAGNAATATCTTTTGACTCTAACCCNNTCACCATTGAAGTAGATGTATTTGNATTCAGAGAGGTACCTNCTAAGGATCAGTTTNCGATTTTCTTCTTGGTTGAGTTCCCTGAAGGCTCNTGCCACTCTCCTNGCAGNGTCTTCGTCTGGGCTCACNTTCAGCTCTTCCATGTCTGCAAANACCTAAAAGAGAANAACCGAAAGAAAATGTATNTTAGAACCANGTCATACTTNTTGCAGAGAGNNGTANAGTANCTTGAGGAGTATCTNNAGNGTAANATCAAGATNAATTCTCGAGGNAAGAAAGAANCTTGTTTTACCTCGATAACCTTATNGTNAAGNTCATGGTGAGCATACAANGCTATCATCCTAGCNAACAANCGTCTTGGNATGGATCGTGTATGTGTGTGNANAATCATGNTCCATAAGGANTCTGCCTCNTCAGCNCTTTCGTNCATATCAAATGCCAATAANAGGNTATCNTATGTTCCCATTGTAGCACCTTGCCCTTTGCTTAACATCCACTTAGCCAACTGAGTTTGTNACTNAACCAATCAACTCAGGACACNATATTTAGCAAAANTTTACACTTGACATAGGAAGNCGTATTACTTACTTGAATCACACGATGCCACTGACTTCTCTTTCTTAAGATTTGCAANGCCTTAGCTGCAGCAATAATGGGAAATTCAACCTCCCAAGCTACCCATTTATTCAAAGCTCCATAAACAGNCTCTTTTTCATTTGGGAGTCCAGAGAGCTACATATCANCAAGNACNACTTTATNATCGTTTCACTTATGAAAACATATGTTNTAACAGAANTTTTCCTCACTTACCATTCGAACAAGATTAAGNGCTTTCTGNCCNGACCCAGCAGAGTCATTCTTCTTCCACAAGTGATGCTCTTTCTTCCCCACTTTNTTTATTTCCTTNCTACAATNTCAACANTCAACACAAANACTTACTGGTGAATCANNCAGNAANAAACNATGTCAAANGATTACTCAAGAAAGATATGCAACCAAACANAAGTTANTTTACNNNNNNNANATATATNACTTGAAATGTAACAAACAACAAACCTGTTCACAGTGGCAACATAACCTCNGTCNAATTTCCCNGCTTCCTTCTCTGANAACTTNAAAACAATATCACCATTATGACATGTTGTNATTCATTTCACAAGCTATATAGCCATTAAACAGANAGTAACTACAAAAACNNNNNTTTNTTACCTTTGATCTGATGCAAAAGCCAAACNTTTTCGTCTTCAACGAGGAGTAACTTCCNCAACGTGNAGATTGAAGCAAAGANNAGCATATAAATTCTTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.140493
PRIMER_LEFT_0_PENALTY=0.066262
PRIMER_RIGHT_0_PENALTY=0.074231
PRIMER_LEFT_0_SEQUENCE=GAAGCATGATCTTGTGGGGT
PRIMER_RIGHT_0_SEQUENCE=GGATGTTAAGCAAAGGGCAA
PRIMER_LEFT_0=330,20
PRIMER_RIGHT_0=1213,20
PRIMER_LEFT_0_TM=59.934
PRIMER_RIGHT_0_TM=60.074
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.6000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=884
PRIMER_PAIR_1_PENALTY=0.186507
PRIMER_LEFT_1_PENALTY=0.066262
PRIMER_RIGHT_1_PENALTY=0.120245
PRIMER_LEFT_1_SEQUENCE=GAAGCATGATCTTGTGGGGT
PRIMER_RIGHT_1_SEQUENCE=TGGCATCGTGTGATTCAAGT
PRIMER_LEFT_1=330,20
PRIMER_RIGHT_1=1324,20
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=60.120
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=995
PRIMER_PAIR_2_PENALTY=0.186507
PRIMER_LEFT_2_PENALTY=0.066262
PRIMER_RIGHT_2_PENALTY=0.120245
PRIMER_LEFT_2_SEQUENCE=GAAGCATGATCTTGTGGGGT
PRIMER_RIGHT_2_SEQUENCE=GTGGCATCGTGTGATTCAAG
PRIMER_LEFT_2=330,20
PRIMER_RIGHT_2=1325,20
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=60.120
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=996
PRIMER_PAIR_3_PENALTY=0.277598
PRIMER_LEFT_3_PENALTY=0.203367
PRIMER_RIGHT_3_PENALTY=0.074231
PRIMER_LEFT_3_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_3_SEQUENCE=GGATGTTAAGCAAAGGGCAA
PRIMER_LEFT_3=333,20
PRIMER_RIGHT_3=1213,20
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=881
PRIMER_PAIR_4_PENALTY=0.287737
PRIMER_LEFT_4_PENALTY=0.213506
PRIMER_RIGHT_4_PENALTY=0.074231
PRIMER_LEFT_4_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_4_SEQUENCE=GGATGTTAAGCAAAGGGCAA
PRIMER_LEFT_4=376,20
PRIMER_RIGHT_4=1213,20
PRIMER_LEFT_4_TM=60.214
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=838
PRIMER_PAIR_5_PENALTY=0.319744
PRIMER_LEFT_5_PENALTY=0.066262
PRIMER_RIGHT_5_PENALTY=0.253482
PRIMER_LEFT_5_SEQUENCE=GAAGCATGATCTTGTGGGGT
PRIMER_RIGHT_5_SEQUENCE=GTTAAGCAAAGGGCAAGGTG
PRIMER_LEFT_5=330,20
PRIMER_RIGHT_5=1209,20
PRIMER_LEFT_5_TM=59.934
PRIMER_RIGHT_5_TM=59.747
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=10.6000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=880
PRIMER_PAIR_6_PENALTY=0.319744
PRIMER_LEFT_6_PENALTY=0.066262
PRIMER_RIGHT_6_PENALTY=0.253482
PRIMER_LEFT_6_SEQUENCE=GAAGCATGATCTTGTGGGGT
PRIMER_RIGHT_6_SEQUENCE=TGTTAAGCAAAGGGCAAGGT
PRIMER_LEFT_6=330,20
PRIMER_RIGHT_6=1210,20
PRIMER_LEFT_6_TM=59.934
PRIMER_RIGHT_6_TM=59.747
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.6000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=881
PRIMER_PAIR_7_PENALTY=0.323611
PRIMER_LEFT_7_PENALTY=0.203367
PRIMER_RIGHT_7_PENALTY=0.120245
PRIMER_LEFT_7_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_7_SEQUENCE=TGGCATCGTGTGATTCAAGT
PRIMER_LEFT_7=333,20
PRIMER_RIGHT_7=1324,20
PRIMER_LEFT_7_TM=59.797
PRIMER_RIGHT_7_TM=60.120
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=992
PRIMER_PAIR_8_PENALTY=0.323611
PRIMER_LEFT_8_PENALTY=0.203367
PRIMER_RIGHT_8_PENALTY=0.120245
PRIMER_LEFT_8_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_8_SEQUENCE=GTGGCATCGTGTGATTCAAG
PRIMER_LEFT_8=333,20
PRIMER_RIGHT_8=1325,20
PRIMER_LEFT_8_TM=59.797
PRIMER_RIGHT_8_TM=60.120
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=993
PRIMER_PAIR_9_PENALTY=0.333750
PRIMER_LEFT_9_PENALTY=0.213506
PRIMER_RIGHT_9_PENALTY=0.120245
PRIMER_LEFT_9_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_9_SEQUENCE=TGGCATCGTGTGATTCAAGT
PRIMER_LEFT_9=376,20
PRIMER_RIGHT_9=1324,20
PRIMER_LEFT_9_TM=60.214
PRIMER_RIGHT_9_TM=60.120
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=949
PRIMER_PAIR_10_PENALTY=0.333750
PRIMER_LEFT_10_PENALTY=0.213506
PRIMER_RIGHT_10_PENALTY=0.120245
PRIMER_LEFT_10_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_10_SEQUENCE=GTGGCATCGTGTGATTCAAG
PRIMER_LEFT_10=376,20
PRIMER_RIGHT_10=1325,20
PRIMER_LEFT_10_TM=60.214
PRIMER_RIGHT_10_TM=60.120
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=950
PRIMER_PAIR_11_PENALTY=0.426041
PRIMER_LEFT_11_PENALTY=0.351810
PRIMER_RIGHT_11_PENALTY=0.074231
PRIMER_LEFT_11_SEQUENCE=TTGAAGCATGATCTTGTGGG
PRIMER_RIGHT_11_SEQUENCE=GGATGTTAAGCAAAGGGCAA
PRIMER_LEFT_11=328,20
PRIMER_RIGHT_11=1213,20
PRIMER_LEFT_11_TM=59.648
PRIMER_RIGHT_11_TM=60.074
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=10.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=886
PRIMER_PAIR_12_PENALTY=0.456849
PRIMER_LEFT_12_PENALTY=0.203367
PRIMER_RIGHT_12_PENALTY=0.253482
PRIMER_LEFT_12_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_12_SEQUENCE=GTTAAGCAAAGGGCAAGGTG
PRIMER_LEFT_12=333,20
PRIMER_RIGHT_12=1209,20
PRIMER_LEFT_12_TM=59.797
PRIMER_RIGHT_12_TM=59.747
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=877
PRIMER_PAIR_13_PENALTY=0.456849
PRIMER_LEFT_13_PENALTY=0.203367
PRIMER_RIGHT_13_PENALTY=0.253482
PRIMER_LEFT_13_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_13_SEQUENCE=TGTTAAGCAAAGGGCAAGGT
PRIMER_LEFT_13=333,20
PRIMER_RIGHT_13=1210,20
PRIMER_LEFT_13_TM=59.797
PRIMER_RIGHT_13_TM=59.747
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=878
PRIMER_PAIR_14_PENALTY=0.466988
PRIMER_LEFT_14_PENALTY=0.213506
PRIMER_RIGHT_14_PENALTY=0.253482
PRIMER_LEFT_14_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_14_SEQUENCE=GTTAAGCAAAGGGCAAGGTG
PRIMER_LEFT_14=376,20
PRIMER_RIGHT_14=1209,20
PRIMER_LEFT_14_TM=60.214
PRIMER_RIGHT_14_TM=59.747
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=834
PRIMER_PAIR_15_PENALTY=0.466988
PRIMER_LEFT_15_PENALTY=0.213506
PRIMER_RIGHT_15_PENALTY=0.253482
PRIMER_LEFT_15_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_15_SEQUENCE=TGTTAAGCAAAGGGCAAGGT
PRIMER_LEFT_15=376,20
PRIMER_RIGHT_15=1210,20
PRIMER_LEFT_15_TM=60.214
PRIMER_RIGHT_15_TM=59.747
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=835
PRIMER_PAIR_16_PENALTY=0.472055
PRIMER_LEFT_16_PENALTY=0.351810
PRIMER_RIGHT_16_PENALTY=0.120245
PRIMER_LEFT_16_SEQUENCE=TTGAAGCATGATCTTGTGGG
PRIMER_RIGHT_16_SEQUENCE=TGGCATCGTGTGATTCAAGT
PRIMER_LEFT_16=328,20
PRIMER_RIGHT_16=1324,20
PRIMER_LEFT_16_TM=59.648
PRIMER_RIGHT_16_TM=60.120
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=997
PRIMER_PAIR_17_PENALTY=0.472055
PRIMER_LEFT_17_PENALTY=0.351810
PRIMER_RIGHT_17_PENALTY=0.120245
PRIMER_LEFT_17_SEQUENCE=TTGAAGCATGATCTTGTGGG
PRIMER_RIGHT_17_SEQUENCE=GTGGCATCGTGTGATTCAAG
PRIMER_LEFT_17=328,20
PRIMER_RIGHT_17=1325,20
PRIMER_LEFT_17_TM=59.648
PRIMER_RIGHT_17_TM=60.120
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=998
PRIMER_PAIR_18_PENALTY=0.509229
PRIMER_LEFT_18_PENALTY=0.066262
PRIMER_RIGHT_18_PENALTY=0.442967
PRIMER_LEFT_18_SEQUENCE=GAAGCATGATCTTGTGGGGT
PRIMER_RIGHT_18_SEQUENCE=GTCAGTGGCATCGTGTGATT
PRIMER_LEFT_18=330,20
PRIMER_RIGHT_18=1329,20
PRIMER_LEFT_18_TM=59.934
PRIMER_RIGHT_18_TM=59.557
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=10.6000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=1000
PRIMER_PAIR_19_PENALTY=0.521948
PRIMER_LEFT_19_PENALTY=0.203367
PRIMER_RIGHT_19_PENALTY=0.318581
PRIMER_LEFT_19_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_19_SEQUENCE=AAGTCAGTGGCATCGTGTGA
PRIMER_LEFT_19=333,20
PRIMER_RIGHT_19=1331,20
PRIMER_LEFT_19_TM=59.797
PRIMER_RIGHT_19_TM=60.319
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=999
PRIMER_PAIR_20_PENALTY=0.521948
PRIMER_LEFT_20_PENALTY=0.203367
PRIMER_RIGHT_20_PENALTY=0.318581
PRIMER_LEFT_20_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_20_SEQUENCE=GAAGTCAGTGGCATCGTGTG
PRIMER_LEFT_20=333,20
PRIMER_RIGHT_20=1332,20
PRIMER_LEFT_20_TM=59.797
PRIMER_RIGHT_20_TM=60.319
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=1000
PRIMER_PAIR_21_PENALTY=0.532086
PRIMER_LEFT_21_PENALTY=0.213506
PRIMER_RIGHT_21_PENALTY=0.318581
PRIMER_LEFT_21_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_21_SEQUENCE=AAGTCAGTGGCATCGTGTGA
PRIMER_LEFT_21=376,20
PRIMER_RIGHT_21=1331,20
PRIMER_LEFT_21_TM=60.214
PRIMER_RIGHT_21_TM=60.319
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=956
PRIMER_PAIR_22_PENALTY=0.532086
PRIMER_LEFT_22_PENALTY=0.213506
PRIMER_RIGHT_22_PENALTY=0.318581
PRIMER_LEFT_22_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_22_SEQUENCE=GAAGTCAGTGGCATCGTGTG
PRIMER_LEFT_22=376,20
PRIMER_RIGHT_22=1332,20
PRIMER_LEFT_22_TM=60.214
PRIMER_RIGHT_22_TM=60.319
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.3000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=957
PRIMER_PAIR_23_PENALTY=0.605292
PRIMER_LEFT_23_PENALTY=0.351810
PRIMER_RIGHT_23_PENALTY=0.253482
PRIMER_LEFT_23_SEQUENCE=TTGAAGCATGATCTTGTGGG
PRIMER_RIGHT_23_SEQUENCE=GTTAAGCAAAGGGCAAGGTG
PRIMER_LEFT_23=328,20
PRIMER_RIGHT_23=1209,20
PRIMER_LEFT_23_TM=59.648
PRIMER_RIGHT_23_TM=59.747
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=882
PRIMER_PAIR_24_PENALTY=0.605292
PRIMER_LEFT_24_PENALTY=0.351810
PRIMER_RIGHT_24_PENALTY=0.253482
PRIMER_LEFT_24_SEQUENCE=TTGAAGCATGATCTTGTGGG
PRIMER_RIGHT_24_SEQUENCE=TGTTAAGCAAAGGGCAAGGT
PRIMER_LEFT_24=328,20
PRIMER_RIGHT_24=1210,20
PRIMER_LEFT_24_TM=59.648
PRIMER_RIGHT_24_TM=59.747
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=883
PRIMER_PAIR_25_PENALTY=0.646334
PRIMER_LEFT_25_PENALTY=0.203367
PRIMER_RIGHT_25_PENALTY=0.442967
PRIMER_LEFT_25_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_25_SEQUENCE=GTCAGTGGCATCGTGTGATT
PRIMER_LEFT_25=333,20
PRIMER_RIGHT_25=1329,20
PRIMER_LEFT_25_TM=59.797
PRIMER_RIGHT_25_TM=59.557
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=997
PRIMER_PAIR_26_PENALTY=0.656472
PRIMER_LEFT_26_PENALTY=0.213506
PRIMER_RIGHT_26_PENALTY=0.442967
PRIMER_LEFT_26_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_26_SEQUENCE=GTCAGTGGCATCGTGTGATT
PRIMER_LEFT_26=376,20
PRIMER_RIGHT_26=1329,20
PRIMER_LEFT_26_TM=60.214
PRIMER_RIGHT_26_TM=59.557
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=954
PRIMER_PAIR_27_PENALTY=0.664134
PRIMER_LEFT_27_PENALTY=0.589903
PRIMER_RIGHT_27_PENALTY=0.074231
PRIMER_LEFT_27_SEQUENCE=AGCATGATCTTGTGGGGTTT
PRIMER_RIGHT_27_SEQUENCE=GGATGTTAAGCAAAGGGCAA
PRIMER_LEFT_27=332,20
PRIMER_RIGHT_27=1213,20
PRIMER_LEFT_27_TM=59.410
PRIMER_RIGHT_27_TM=60.074
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=10.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=882
PRIMER_PAIR_28_PENALTY=0.664134
PRIMER_LEFT_28_PENALTY=0.589903
PRIMER_RIGHT_28_PENALTY=0.074231
PRIMER_LEFT_28_SEQUENCE=AAGCATGATCTTGTGGGGTT
PRIMER_RIGHT_28_SEQUENCE=GGATGTTAAGCAAAGGGCAA
PRIMER_LEFT_28=331,20
PRIMER_RIGHT_28=1213,20
PRIMER_LEFT_28_TM=59.410
PRIMER_RIGHT_28_TM=60.074
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=883
PRIMER_PAIR_29_PENALTY=0.684813
PRIMER_LEFT_29_PENALTY=0.610582
PRIMER_RIGHT_29_PENALTY=0.074231
PRIMER_LEFT_29_SEQUENCE=TGATCTTGTGGGGTTTTGTG
PRIMER_RIGHT_29_SEQUENCE=GGATGTTAAGCAAAGGGCAA
PRIMER_LEFT_29=336,20
PRIMER_RIGHT_29=1213,20
PRIMER_LEFT_29_TM=59.389
PRIMER_RIGHT_29_TM=60.074
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=10.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=878
PRIMER_PAIR_30_PENALTY=0.710147
PRIMER_LEFT_30_PENALTY=0.589903
PRIMER_RIGHT_30_PENALTY=0.120245
PRIMER_LEFT_30_SEQUENCE=AGCATGATCTTGTGGGGTTT
PRIMER_RIGHT_30_SEQUENCE=TGGCATCGTGTGATTCAAGT
PRIMER_LEFT_30=332,20
PRIMER_RIGHT_30=1324,20
PRIMER_LEFT_30_TM=59.410
PRIMER_RIGHT_30_TM=60.120
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=993
PRIMER_PAIR_31_PENALTY=0.710147
PRIMER_LEFT_31_PENALTY=0.589903
PRIMER_RIGHT_31_PENALTY=0.120245
PRIMER_LEFT_31_SEQUENCE=AGCATGATCTTGTGGGGTTT
PRIMER_RIGHT_31_SEQUENCE=GTGGCATCGTGTGATTCAAG
PRIMER_LEFT_31=332,20
PRIMER_RIGHT_31=1325,20
PRIMER_LEFT_31_TM=59.410
PRIMER_RIGHT_31_TM=60.120
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=994
PRIMER_PAIR_32_PENALTY=0.710147
PRIMER_LEFT_32_PENALTY=0.589903
PRIMER_RIGHT_32_PENALTY=0.120245
PRIMER_LEFT_32_SEQUENCE=AAGCATGATCTTGTGGGGTT
PRIMER_RIGHT_32_SEQUENCE=TGGCATCGTGTGATTCAAGT
PRIMER_LEFT_32=331,20
PRIMER_RIGHT_32=1324,20
PRIMER_LEFT_32_TM=59.410
PRIMER_RIGHT_32_TM=60.120
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=994
PRIMER_PAIR_33_PENALTY=0.710147
PRIMER_LEFT_33_PENALTY=0.589903
PRIMER_RIGHT_33_PENALTY=0.120245
PRIMER_LEFT_33_SEQUENCE=AAGCATGATCTTGTGGGGTT
PRIMER_RIGHT_33_SEQUENCE=GTGGCATCGTGTGATTCAAG
PRIMER_LEFT_33=331,20
PRIMER_RIGHT_33=1325,20
PRIMER_LEFT_33_TM=59.410
PRIMER_RIGHT_33_TM=60.120
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=995
PRIMER_PAIR_34_PENALTY=0.730827
PRIMER_LEFT_34_PENALTY=0.610582
PRIMER_RIGHT_34_PENALTY=0.120245
PRIMER_LEFT_34_SEQUENCE=TGATCTTGTGGGGTTTTGTG
PRIMER_RIGHT_34_SEQUENCE=TGGCATCGTGTGATTCAAGT
PRIMER_LEFT_34=336,20
PRIMER_RIGHT_34=1324,20
PRIMER_LEFT_34_TM=59.389
PRIMER_RIGHT_34_TM=60.120
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=989
PRIMER_PAIR_35_PENALTY=0.730827
PRIMER_LEFT_35_PENALTY=0.610582
PRIMER_RIGHT_35_PENALTY=0.120245
PRIMER_LEFT_35_SEQUENCE=TGATCTTGTGGGGTTTTGTG
PRIMER_RIGHT_35_SEQUENCE=GTGGCATCGTGTGATTCAAG
PRIMER_LEFT_35=336,20
PRIMER_RIGHT_35=1325,20
PRIMER_LEFT_35_TM=59.389
PRIMER_RIGHT_35_TM=60.120
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=990
PRIMER_PAIR_36_PENALTY=0.841467
PRIMER_LEFT_36_PENALTY=0.066262
PRIMER_RIGHT_36_PENALTY=0.775205
PRIMER_LEFT_36_SEQUENCE=GAAGCATGATCTTGTGGGGT
PRIMER_RIGHT_36_SEQUENCE=ATGTTAAGCAAAGGGCAAGG
PRIMER_LEFT_36=330,20
PRIMER_RIGHT_36=1211,20
PRIMER_LEFT_36_TM=59.934
PRIMER_RIGHT_36_TM=59.225
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.6000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=882
PRIMER_PAIR_37_PENALTY=0.843385
PRIMER_LEFT_37_PENALTY=0.589903
PRIMER_RIGHT_37_PENALTY=0.253482
PRIMER_LEFT_37_SEQUENCE=AGCATGATCTTGTGGGGTTT
PRIMER_RIGHT_37_SEQUENCE=GTTAAGCAAAGGGCAAGGTG
PRIMER_LEFT_37=332,20
PRIMER_RIGHT_37=1209,20
PRIMER_LEFT_37_TM=59.410
PRIMER_RIGHT_37_TM=59.747
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=878
PRIMER_PAIR_38_PENALTY=0.843385
PRIMER_LEFT_38_PENALTY=0.589903
PRIMER_RIGHT_38_PENALTY=0.253482
PRIMER_LEFT_38_SEQUENCE=AGCATGATCTTGTGGGGTTT
PRIMER_RIGHT_38_SEQUENCE=TGTTAAGCAAAGGGCAAGGT
PRIMER_LEFT_38=332,20
PRIMER_RIGHT_38=1210,20
PRIMER_LEFT_38_TM=59.410
PRIMER_RIGHT_38_TM=59.747
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=879
PRIMER_PAIR_39_PENALTY=0.843385
PRIMER_LEFT_39_PENALTY=0.589903
PRIMER_RIGHT_39_PENALTY=0.253482
PRIMER_LEFT_39_SEQUENCE=AAGCATGATCTTGTGGGGTT
PRIMER_RIGHT_39_SEQUENCE=GTTAAGCAAAGGGCAAGGTG
PRIMER_LEFT_39=331,20
PRIMER_RIGHT_39=1209,20
PRIMER_LEFT_39_TM=59.410
PRIMER_RIGHT_39_TM=59.747
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=879
PRIMER_PAIR_40_PENALTY=0.843385
PRIMER_LEFT_40_PENALTY=0.589903
PRIMER_RIGHT_40_PENALTY=0.253482
PRIMER_LEFT_40_SEQUENCE=AAGCATGATCTTGTGGGGTT
PRIMER_RIGHT_40_SEQUENCE=TGTTAAGCAAAGGGCAAGGT
PRIMER_LEFT_40=331,20
PRIMER_RIGHT_40=1210,20
PRIMER_LEFT_40_TM=59.410
PRIMER_RIGHT_40_TM=59.747
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=880
PRIMER_PAIR_41_PENALTY=0.864064
PRIMER_LEFT_41_PENALTY=0.610582
PRIMER_RIGHT_41_PENALTY=0.253482
PRIMER_LEFT_41_SEQUENCE=TGATCTTGTGGGGTTTTGTG
PRIMER_RIGHT_41_SEQUENCE=GTTAAGCAAAGGGCAAGGTG
PRIMER_LEFT_41=336,20
PRIMER_RIGHT_41=1209,20
PRIMER_LEFT_41_TM=59.389
PRIMER_RIGHT_41_TM=59.747
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=874
PRIMER_PAIR_42_PENALTY=0.864064
PRIMER_LEFT_42_PENALTY=0.610582
PRIMER_RIGHT_42_PENALTY=0.253482
PRIMER_LEFT_42_SEQUENCE=TGATCTTGTGGGGTTTTGTG
PRIMER_RIGHT_42_SEQUENCE=TGTTAAGCAAAGGGCAAGGT
PRIMER_LEFT_42=336,20
PRIMER_RIGHT_42=1210,20
PRIMER_LEFT_42_TM=59.389
PRIMER_RIGHT_42_TM=59.747
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=875
PRIMER_PAIR_43_PENALTY=0.908484
PRIMER_LEFT_43_PENALTY=0.589903
PRIMER_RIGHT_43_PENALTY=0.318581
PRIMER_LEFT_43_SEQUENCE=AGCATGATCTTGTGGGGTTT
PRIMER_RIGHT_43_SEQUENCE=AAGTCAGTGGCATCGTGTGA
PRIMER_LEFT_43=332,20
PRIMER_RIGHT_43=1331,20
PRIMER_LEFT_43_TM=59.410
PRIMER_RIGHT_43_TM=60.319
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=1000
PRIMER_PAIR_44_PENALTY=0.929163
PRIMER_LEFT_44_PENALTY=0.610582
PRIMER_RIGHT_44_PENALTY=0.318581
PRIMER_LEFT_44_SEQUENCE=TGATCTTGTGGGGTTTTGTG
PRIMER_RIGHT_44_SEQUENCE=AAGTCAGTGGCATCGTGTGA
PRIMER_LEFT_44=336,20
PRIMER_RIGHT_44=1331,20
PRIMER_LEFT_44_TM=59.389
PRIMER_RIGHT_44_TM=60.319
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=996
PRIMER_PAIR_45_PENALTY=0.929163
PRIMER_LEFT_45_PENALTY=0.610582
PRIMER_RIGHT_45_PENALTY=0.318581
PRIMER_LEFT_45_SEQUENCE=TGATCTTGTGGGGTTTTGTG
PRIMER_RIGHT_45_SEQUENCE=GAAGTCAGTGGCATCGTGTG
PRIMER_LEFT_45=336,20
PRIMER_RIGHT_45=1332,20
PRIMER_LEFT_45_TM=59.389
PRIMER_RIGHT_45_TM=60.319
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=997
PRIMER_PAIR_46_PENALTY=0.978572
PRIMER_LEFT_46_PENALTY=0.203367
PRIMER_RIGHT_46_PENALTY=0.775205
PRIMER_LEFT_46_SEQUENCE=GCATGATCTTGTGGGGTTTT
PRIMER_RIGHT_46_SEQUENCE=ATGTTAAGCAAAGGGCAAGG
PRIMER_LEFT_46=333,20
PRIMER_RIGHT_46=1211,20
PRIMER_LEFT_46_TM=59.797
PRIMER_RIGHT_46_TM=59.225
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=879
PRIMER_PAIR_47_PENALTY=0.988711
PRIMER_LEFT_47_PENALTY=0.213506
PRIMER_RIGHT_47_PENALTY=0.775205
PRIMER_LEFT_47_SEQUENCE=CAGCCATGCTGGATTCTTTT
PRIMER_RIGHT_47_SEQUENCE=ATGTTAAGCAAAGGGCAAGG
PRIMER_LEFT_47=376,20
PRIMER_RIGHT_47=1211,20
PRIMER_LEFT_47_TM=60.214
PRIMER_RIGHT_47_TM=59.225
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.3000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=836
PRIMER_PAIR_48_PENALTY=1.032870
PRIMER_LEFT_48_PENALTY=0.589903
PRIMER_RIGHT_48_PENALTY=0.442967
PRIMER_LEFT_48_SEQUENCE=AGCATGATCTTGTGGGGTTT
PRIMER_RIGHT_48_SEQUENCE=GTCAGTGGCATCGTGTGATT
PRIMER_LEFT_48=332,20
PRIMER_RIGHT_48=1329,20
PRIMER_LEFT_48_TM=59.410
PRIMER_RIGHT_48_TM=59.557
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=998
PRIMER_PAIR_49_PENALTY=1.032870
PRIMER_LEFT_49_PENALTY=0.589903
PRIMER_RIGHT_49_PENALTY=0.442967
PRIMER_LEFT_49_SEQUENCE=AAGCATGATCTTGTGGGGTT
PRIMER_RIGHT_49_SEQUENCE=GTCAGTGGCATCGTGTGATT
PRIMER_LEFT_49=331,20
PRIMER_RIGHT_49=1329,20
PRIMER_LEFT_49_TM=59.410
PRIMER_RIGHT_49_TM=59.557
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=999
=
SEQUENCE_ID=MAGIC.329_3_14399230_14399239_hi-0_tsu-0_11074_56500_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_14613552_14613880_no-0_zu-0_86014_23640_TRUE
SEQUENCE_TARGET=950,428
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAATTTGTCCTTCTTAAGGANATCTCGAACTGAATAGGAACTTTGAGGNTTTAACATAACTGTNNNAAAAAAGCATAAANNNNNNNNNNNTTCANAAATTTTAGAATTCTTAACAATTAAGAAAACTAACACTATANTCAAAACANANTTTCCTCTAATGANTACAATGATCCAAGATCCATATTTCACTAGAATNCAAGTGAGCTTTGNCTCCACCCTTGAATATCTAGTTATTTAGGTAAGCAACATCTTGCTAATTATATCNTATATAATTCTTGGTTNTNTATGACATCTAATNTCTTATCCAACCCTATGTCTCTAAGNCCANAACCNTTTTGATAGNTTAGTCAAGTAAACTANNTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_21390244_21390273_ws-0_sf-2_65489_131815_TRUE
SEQUENCE_TARGET=950,129
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAGATATGTAAGTGGANAAGTCTCTCANTAGTGTACNATATGNGAGNNATTGCTTCTATTTATAAAGTTTTATAAGGTTGATAATANATTTTTGGANGGNAATNNNNTANTNTTCGGAGGNCGATCCAAATATTTAGCTCAAANTNTTTTGGAAAAGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_9995389_9995746_oy-0_kn-0_55659_24804_TRUE
SEQUENCE_TARGET=950,457
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_2576672_2576758_po-0_can-0_87122_2907_TRUE
SEQUENCE_TARGET=950,186
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGTTGGTTTAGAACCAACTTGAAACCGTCTTGATCCNTTTGGTACAAGTTTAATTTGAAGGATTTTAAAAGAGATNAAANTTTTTGACNGCCGAAANTGTGGNNNGNAGGTCCTTTGGATATANANACANAATAAAANAATTNNNNNNNNNNNNNNNNGNGTTNGGACTCAAATAATATNTGGGTATGGTTTGGTATGGNNACAGGTANAGGCATNTGGGACAATTTGCACCACTGGGAGGGGANCAAACGGCNGCACANCTTCCAGGAGATTGGGTTTCNATTGGACACAGCAGCCAGTGGCTCTGGTANTCCGTCTACGCCAGGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATCCACNAANGAATNTTTTNANTTTNTACTNAANNTAGAAAATGTTTNGATNTGGAACAGTAAGCAAGTGAGCTGGCGTACGAGAGTGCTNGTNGTTTCAGATTGGATGAGGCGTTTCATCTTTGGTAGNGATTCCAGTAGCATCTGATTTTCAANCTCCAAGGCTNCAANACACNNNTCTCTNTNTNTCACGAGTTACACTAACNTTCNCATGANGAAAAAGNTTTGATTCACTNNNAAAANNNGAAATCCAAATCGGNTTTTATATTTTGTAATAAAATAATGACCACANGTAACGAGGAGAGANTNNCTCGTTTCGTTGGCCATATCGTTGCTGANCCNGAGGANTNTACTCTCTGNTTTTCTCTNCAAATATTNTTCAGGTTGGCTTTTTACCTTTATATCCTCNTTTTTTGTAAGCNNTTTCNTCAAATTTTGTTTAGTACGATGACTAAAACTGAGTTTTGTTATTTCTTGNTCTCTGTTTTTCTCCTTCAATATCATGGTCTNTTTNTNTNTTCAAAATATTGGTATTTTTNATATCGTGGATTAGCTNAAAATGGNTTTAANTNNTTNTNNANNAAAGANATGGAGTGANNAAAAANNTTTCANNNNNNTTNTTTATATTAAAGTGGCTAGGAAAACAGAGAAATTACATAATCAANNNTTNAAAAGTTGAATTTNAANAAGTNNATCTAGAATAAGACTTGTTTTTTTAAGTGTCGTCAACATCTGTGACGCTGATAATATNAGCGAAACCAACTTTCTTGCGAAGCCGGANAACTAAACATGCGGCNTCGACACCTTCTCCGATCACCACCACTTTGTCTTTCTCTTCTCCTTCTATTCCCACAAANGTCACTCCTGCTCCNTTATTNAAACATGAGAANATGAGATCATTCCATCAAGCGAATTAGAACGAANATAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATATCTTACCAGTNGTTTTGGCTCCNATCTTGAGNGCTTCTGAACGACATTTGTCGCACCTCATACTTANCGNCATCAAGATTTTCTGCNCANNAAANTTCCAATNTTNCAAATAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTACCTTGGCCANTTTGGAACCTCTTNGAGAAAATGAAACNTTNTTTTNNNNNTCCTTTTNGTATAANTGATNNNTNTAATTTACGTGTGTANATATANAAGTGNTNCTGTNNTTGTCTCATTTTNTTATGGCANATNGNTTAACNNNNNNNNNNANNCNTATTTNATNTGTACTATTANTTGCNANGAANTCGCANAGTAATTTTCATGNAACTGCTTCGTANTTAATATTAGTNNANATNTNNACGTTTAANTGGACAATATANGGTATTGCTTGACTCANAAGGGTTAGANTTGGTNAGGTAAAGGNTAGATTTTTNTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.104657
PRIMER_LEFT_0_PENALTY=0.073614
PRIMER_RIGHT_0_PENALTY=0.031043
PRIMER_LEFT_0_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_0_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_0=509,20
PRIMER_RIGHT_0=1222,20
PRIMER_LEFT_0_TM=60.074
PRIMER_RIGHT_0_TM=59.969
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=714
PRIMER_PAIR_1_PENALTY=0.185428
PRIMER_LEFT_1_PENALTY=0.073614
PRIMER_RIGHT_1_PENALTY=0.111814
PRIMER_LEFT_1_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_1_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_1=509,20
PRIMER_RIGHT_1=1226,20
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.112
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=718
PRIMER_PAIR_2_PENALTY=0.574838
PRIMER_LEFT_2_PENALTY=0.073614
PRIMER_RIGHT_2_PENALTY=0.501224
PRIMER_LEFT_2_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_2_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_2=509,20
PRIMER_RIGHT_2=1173,20
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.499
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=665
PRIMER_PAIR_3_PENALTY=0.583945
PRIMER_LEFT_3_PENALTY=0.073614
PRIMER_RIGHT_3_PENALTY=0.510331
PRIMER_LEFT_3_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_3_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_3=509,20
PRIMER_RIGHT_3=1227,20
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.510
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=719
PRIMER_PAIR_4_PENALTY=0.583945
PRIMER_LEFT_4_PENALTY=0.073614
PRIMER_RIGHT_4_PENALTY=0.510331
PRIMER_LEFT_4_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_4_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_4=509,20
PRIMER_RIGHT_4=1228,20
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.510
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=720
PRIMER_PAIR_5_PENALTY=0.634202
PRIMER_LEFT_5_PENALTY=0.603160
PRIMER_RIGHT_5_PENALTY=0.031043
PRIMER_LEFT_5_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_5_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_5=500,20
PRIMER_RIGHT_5=1222,20
PRIMER_LEFT_5_TM=60.603
PRIMER_RIGHT_5_TM=59.969
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.7000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=723
PRIMER_PAIR_6_PENALTY=0.634202
PRIMER_LEFT_6_PENALTY=0.603160
PRIMER_RIGHT_6_PENALTY=0.031043
PRIMER_LEFT_6_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_6_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_6=499,20
PRIMER_RIGHT_6=1222,20
PRIMER_LEFT_6_TM=60.603
PRIMER_RIGHT_6_TM=59.969
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=724
PRIMER_PAIR_7_PENALTY=0.667075
PRIMER_LEFT_7_PENALTY=0.636033
PRIMER_RIGHT_7_PENALTY=0.031043
PRIMER_LEFT_7_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_7_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_7=510,20
PRIMER_RIGHT_7=1222,20
PRIMER_LEFT_7_TM=60.636
PRIMER_RIGHT_7_TM=59.969
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=713
PRIMER_PAIR_8_PENALTY=0.714974
PRIMER_LEFT_8_PENALTY=0.603160
PRIMER_RIGHT_8_PENALTY=0.111814
PRIMER_LEFT_8_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_8_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_8=500,20
PRIMER_RIGHT_8=1226,20
PRIMER_LEFT_8_TM=60.603
PRIMER_RIGHT_8_TM=60.112
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=727
PRIMER_PAIR_9_PENALTY=0.714974
PRIMER_LEFT_9_PENALTY=0.603160
PRIMER_RIGHT_9_PENALTY=0.111814
PRIMER_LEFT_9_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_9_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_9=499,20
PRIMER_RIGHT_9=1226,20
PRIMER_LEFT_9_TM=60.603
PRIMER_RIGHT_9_TM=60.112
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=728
PRIMER_PAIR_10_PENALTY=0.747846
PRIMER_LEFT_10_PENALTY=0.636033
PRIMER_RIGHT_10_PENALTY=0.111814
PRIMER_LEFT_10_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_10_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_10=510,20
PRIMER_RIGHT_10=1226,20
PRIMER_LEFT_10_TM=60.636
PRIMER_RIGHT_10_TM=60.112
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=717
PRIMER_PAIR_11_PENALTY=0.985440
PRIMER_LEFT_11_PENALTY=0.954397
PRIMER_RIGHT_11_PENALTY=0.031043
PRIMER_LEFT_11_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_11_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_11=260,20
PRIMER_RIGHT_11=1222,20
PRIMER_LEFT_11_TM=60.954
PRIMER_RIGHT_11_TM=59.969
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=963
PRIMER_PAIR_12_PENALTY=1.066211
PRIMER_LEFT_12_PENALTY=0.954397
PRIMER_RIGHT_12_PENALTY=0.111814
PRIMER_LEFT_12_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_12_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_12=260,20
PRIMER_RIGHT_12=1226,20
PRIMER_LEFT_12_TM=60.954
PRIMER_RIGHT_12_TM=60.112
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=967
PRIMER_PAIR_13_PENALTY=1.104384
PRIMER_LEFT_13_PENALTY=0.603160
PRIMER_RIGHT_13_PENALTY=0.501224
PRIMER_LEFT_13_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_13_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_13=500,20
PRIMER_RIGHT_13=1173,20
PRIMER_LEFT_13_TM=60.603
PRIMER_RIGHT_13_TM=59.499
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.7000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=674
PRIMER_PAIR_14_PENALTY=1.104384
PRIMER_LEFT_14_PENALTY=0.603160
PRIMER_RIGHT_14_PENALTY=0.501224
PRIMER_LEFT_14_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_14_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_14=499,20
PRIMER_RIGHT_14=1173,20
PRIMER_LEFT_14_TM=60.603
PRIMER_RIGHT_14_TM=59.499
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=675
PRIMER_PAIR_15_PENALTY=1.113491
PRIMER_LEFT_15_PENALTY=0.603160
PRIMER_RIGHT_15_PENALTY=0.510331
PRIMER_LEFT_15_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_15_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_15=500,20
PRIMER_RIGHT_15=1227,20
PRIMER_LEFT_15_TM=60.603
PRIMER_RIGHT_15_TM=60.510
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=728
PRIMER_PAIR_16_PENALTY=1.113491
PRIMER_LEFT_16_PENALTY=0.603160
PRIMER_RIGHT_16_PENALTY=0.510331
PRIMER_LEFT_16_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_16_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_16=500,20
PRIMER_RIGHT_16=1228,20
PRIMER_LEFT_16_TM=60.603
PRIMER_RIGHT_16_TM=60.510
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=729
PRIMER_PAIR_17_PENALTY=1.113491
PRIMER_LEFT_17_PENALTY=0.603160
PRIMER_RIGHT_17_PENALTY=0.510331
PRIMER_LEFT_17_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_17_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_17=499,20
PRIMER_RIGHT_17=1227,20
PRIMER_LEFT_17_TM=60.603
PRIMER_RIGHT_17_TM=60.510
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=729
PRIMER_PAIR_18_PENALTY=1.113491
PRIMER_LEFT_18_PENALTY=0.603160
PRIMER_RIGHT_18_PENALTY=0.510331
PRIMER_LEFT_18_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_18_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_18=499,20
PRIMER_RIGHT_18=1228,20
PRIMER_LEFT_18_TM=60.603
PRIMER_RIGHT_18_TM=60.510
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=730
PRIMER_PAIR_19_PENALTY=1.137257
PRIMER_LEFT_19_PENALTY=0.636033
PRIMER_RIGHT_19_PENALTY=0.501224
PRIMER_LEFT_19_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_19_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_19=510,20
PRIMER_RIGHT_19=1173,20
PRIMER_LEFT_19_TM=60.636
PRIMER_RIGHT_19_TM=59.499
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=664
PRIMER_PAIR_20_PENALTY=1.146364
PRIMER_LEFT_20_PENALTY=0.636033
PRIMER_RIGHT_20_PENALTY=0.510331
PRIMER_LEFT_20_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_20_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_20=510,20
PRIMER_RIGHT_20=1227,20
PRIMER_LEFT_20_TM=60.636
PRIMER_RIGHT_20_TM=60.510
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=718
PRIMER_PAIR_21_PENALTY=1.146364
PRIMER_LEFT_21_PENALTY=0.636033
PRIMER_RIGHT_21_PENALTY=0.510331
PRIMER_LEFT_21_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_21_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_21=510,20
PRIMER_RIGHT_21=1228,20
PRIMER_LEFT_21_TM=60.636
PRIMER_RIGHT_21_TM=60.510
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=719
PRIMER_PAIR_22_PENALTY=1.156363
PRIMER_LEFT_22_PENALTY=0.073614
PRIMER_RIGHT_22_PENALTY=1.082750
PRIMER_LEFT_22_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_22_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_22=509,20
PRIMER_RIGHT_22=1221,20
PRIMER_LEFT_22_TM=60.074
PRIMER_RIGHT_22_TM=61.083
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.8000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=713
PRIMER_PAIR_23_PENALTY=1.289680
PRIMER_LEFT_23_PENALTY=0.073614
PRIMER_RIGHT_23_PENALTY=1.216066
PRIMER_LEFT_23_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_23_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_23=509,20
PRIMER_RIGHT_23=1323,21
PRIMER_LEFT_23_TM=60.074
PRIMER_RIGHT_23_TM=60.216
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.8000
PRIMER_RIGHT_23_END_STABILITY=8.1000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=815
PRIMER_PAIR_24_PENALTY=1.289680
PRIMER_LEFT_24_PENALTY=0.073614
PRIMER_RIGHT_24_PENALTY=1.216066
PRIMER_LEFT_24_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_24_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_24=509,20
PRIMER_RIGHT_24=1324,21
PRIMER_LEFT_24_TM=60.074
PRIMER_RIGHT_24_TM=60.216
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=816
PRIMER_PAIR_25_PENALTY=1.455622
PRIMER_LEFT_25_PENALTY=0.954397
PRIMER_RIGHT_25_PENALTY=0.501224
PRIMER_LEFT_25_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_25_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_25=260,20
PRIMER_RIGHT_25=1173,20
PRIMER_LEFT_25_TM=60.954
PRIMER_RIGHT_25_TM=59.499
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=914
PRIMER_PAIR_26_PENALTY=1.456661
PRIMER_LEFT_26_PENALTY=0.073614
PRIMER_RIGHT_26_PENALTY=1.383047
PRIMER_LEFT_26_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_26_SEQUENCE=GACAAAGTGGTGGTGATCGG
PRIMER_LEFT_26=509,20
PRIMER_RIGHT_26=1231,20
PRIMER_LEFT_26_TM=60.074
PRIMER_RIGHT_26_TM=61.383
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.8000
PRIMER_RIGHT_26_END_STABILITY=9.8000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=723
PRIMER_PAIR_27_PENALTY=1.463306
PRIMER_LEFT_27_PENALTY=1.432264
PRIMER_RIGHT_27_PENALTY=0.031043
PRIMER_LEFT_27_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_27_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_27=300,20
PRIMER_RIGHT_27=1222,20
PRIMER_LEFT_27_TM=58.568
PRIMER_RIGHT_27_TM=59.969
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=923
PRIMER_PAIR_28_PENALTY=1.464728
PRIMER_LEFT_28_PENALTY=0.954397
PRIMER_RIGHT_28_PENALTY=0.510331
PRIMER_LEFT_28_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_28_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_28=260,20
PRIMER_RIGHT_28=1227,20
PRIMER_LEFT_28_TM=60.954
PRIMER_RIGHT_28_TM=60.510
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=968
PRIMER_PAIR_29_PENALTY=1.464728
PRIMER_LEFT_29_PENALTY=0.954397
PRIMER_RIGHT_29_PENALTY=0.510331
PRIMER_LEFT_29_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_29_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_29=260,20
PRIMER_RIGHT_29=1228,20
PRIMER_LEFT_29_TM=60.954
PRIMER_RIGHT_29_TM=60.510
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=7.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=969
PRIMER_PAIR_30_PENALTY=1.544077
PRIMER_LEFT_30_PENALTY=1.432264
PRIMER_RIGHT_30_PENALTY=0.111814
PRIMER_LEFT_30_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_30_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_30=300,20
PRIMER_RIGHT_30=1226,20
PRIMER_LEFT_30_TM=58.568
PRIMER_RIGHT_30_TM=60.112
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=927
PRIMER_PAIR_31_PENALTY=1.586605
PRIMER_LEFT_31_PENALTY=0.073614
PRIMER_RIGHT_31_PENALTY=1.512991
PRIMER_LEFT_31_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_31_SEQUENCE=CAAAGTGGTGGTGATCGGAG
PRIMER_LEFT_31=509,20
PRIMER_RIGHT_31=1229,20
PRIMER_LEFT_31_TM=60.074
PRIMER_RIGHT_31_TM=61.513
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.8000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=721
PRIMER_PAIR_32_PENALTY=1.685909
PRIMER_LEFT_32_PENALTY=0.603160
PRIMER_RIGHT_32_PENALTY=1.082750
PRIMER_LEFT_32_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_32_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_32=500,20
PRIMER_RIGHT_32=1221,20
PRIMER_LEFT_32_TM=60.603
PRIMER_RIGHT_32_TM=61.083
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.7000
PRIMER_RIGHT_32_END_STABILITY=6.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=722
PRIMER_PAIR_33_PENALTY=1.685909
PRIMER_LEFT_33_PENALTY=0.603160
PRIMER_RIGHT_33_PENALTY=1.082750
PRIMER_LEFT_33_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_33_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_33=499,20
PRIMER_RIGHT_33=1221,20
PRIMER_LEFT_33_TM=60.603
PRIMER_RIGHT_33_TM=61.083
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.9000
PRIMER_RIGHT_33_END_STABILITY=6.1000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=723
PRIMER_PAIR_34_PENALTY=1.686595
PRIMER_LEFT_34_PENALTY=0.073614
PRIMER_RIGHT_34_PENALTY=1.612981
PRIMER_LEFT_34_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_34_SEQUENCE=CGTTCTAATTCGCTTGATGG
PRIMER_LEFT_34=509,20
PRIMER_RIGHT_34=1323,20
PRIMER_LEFT_34_TM=60.074
PRIMER_RIGHT_34_TM=58.387
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=8.1000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=815
PRIMER_PAIR_35_PENALTY=1.718782
PRIMER_LEFT_35_PENALTY=0.636033
PRIMER_RIGHT_35_PENALTY=1.082750
PRIMER_LEFT_35_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_35_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_35=510,20
PRIMER_RIGHT_35=1221,20
PRIMER_LEFT_35_TM=60.636
PRIMER_RIGHT_35_TM=61.083
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=712
PRIMER_PAIR_36_PENALTY=1.742272
PRIMER_LEFT_36_PENALTY=0.073614
PRIMER_RIGHT_36_PENALTY=1.668658
PRIMER_LEFT_36_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_36_SEQUENCE=GGCTTCGCAAGAAAGTTGGT
PRIMER_LEFT_36=509,20
PRIMER_RIGHT_36=1179,20
PRIMER_LEFT_36_TM=60.074
PRIMER_RIGHT_36_TM=61.669
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.8000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=671
PRIMER_PAIR_37_PENALTY=1.799825
PRIMER_LEFT_37_PENALTY=1.768783
PRIMER_RIGHT_37_PENALTY=0.031043
PRIMER_LEFT_37_SEQUENCE=TTTCGTTGGCCATATCGTTG
PRIMER_RIGHT_37_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_37=717,20
PRIMER_RIGHT_37=1222,20
PRIMER_LEFT_37_TM=61.769
PRIMER_RIGHT_37_TM=59.969
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=506
PRIMER_PAIR_38_PENALTY=1.819226
PRIMER_LEFT_38_PENALTY=0.603160
PRIMER_RIGHT_38_PENALTY=1.216066
PRIMER_LEFT_38_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_38_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_38=500,20
PRIMER_RIGHT_38=1323,21
PRIMER_LEFT_38_TM=60.603
PRIMER_RIGHT_38_TM=60.216
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.7000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=824
PRIMER_PAIR_39_PENALTY=1.819226
PRIMER_LEFT_39_PENALTY=0.603160
PRIMER_RIGHT_39_PENALTY=1.216066
PRIMER_LEFT_39_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_39_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_39=500,20
PRIMER_RIGHT_39=1324,21
PRIMER_LEFT_39_TM=60.603
PRIMER_RIGHT_39_TM=60.216
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.7000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=825
PRIMER_PAIR_40_PENALTY=1.819226
PRIMER_LEFT_40_PENALTY=0.603160
PRIMER_RIGHT_40_PENALTY=1.216066
PRIMER_LEFT_40_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_40_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_40=499,20
PRIMER_RIGHT_40=1323,21
PRIMER_LEFT_40_TM=60.603
PRIMER_RIGHT_40_TM=60.216
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=825
PRIMER_PAIR_41_PENALTY=1.819226
PRIMER_LEFT_41_PENALTY=0.603160
PRIMER_RIGHT_41_PENALTY=1.216066
PRIMER_LEFT_41_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_41_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_41=499,20
PRIMER_RIGHT_41=1324,21
PRIMER_LEFT_41_TM=60.603
PRIMER_RIGHT_41_TM=60.216
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=826
PRIMER_PAIR_42_PENALTY=1.838051
PRIMER_LEFT_42_PENALTY=0.073614
PRIMER_RIGHT_42_PENALTY=1.764437
PRIMER_LEFT_42_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_42_SEQUENCE=GCTTCGCAAGAAAGTTGGTTT
PRIMER_LEFT_42=509,20
PRIMER_RIGHT_42=1178,21
PRIMER_LEFT_42_TM=60.074
PRIMER_RIGHT_42_TM=60.764
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.8000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=670
PRIMER_PAIR_43_PENALTY=1.852099
PRIMER_LEFT_43_PENALTY=0.636033
PRIMER_RIGHT_43_PENALTY=1.216066
PRIMER_LEFT_43_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_43_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_43=510,20
PRIMER_RIGHT_43=1323,21
PRIMER_LEFT_43_TM=60.636
PRIMER_RIGHT_43_TM=60.216
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=8.1000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=814
PRIMER_PAIR_44_PENALTY=1.852099
PRIMER_LEFT_44_PENALTY=0.636033
PRIMER_RIGHT_44_PENALTY=1.216066
PRIMER_LEFT_44_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_44_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_44=510,20
PRIMER_RIGHT_44=1324,21
PRIMER_LEFT_44_TM=60.636
PRIMER_RIGHT_44_TM=60.216
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=815
PRIMER_PAIR_45_PENALTY=1.880597
PRIMER_LEFT_45_PENALTY=1.768783
PRIMER_RIGHT_45_PENALTY=0.111814
PRIMER_LEFT_45_SEQUENCE=TTTCGTTGGCCATATCGTTG
PRIMER_RIGHT_45_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_45=717,20
PRIMER_RIGHT_45=1226,20
PRIMER_LEFT_45_TM=61.769
PRIMER_RIGHT_45_TM=60.112
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.7000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=510
PRIMER_PAIR_46_PENALTY=1.901384
PRIMER_LEFT_46_PENALTY=1.870342
PRIMER_RIGHT_46_PENALTY=0.031043
PRIMER_LEFT_46_SEQUENCE=ATGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_46_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_46=509,21
PRIMER_RIGHT_46=1222,20
PRIMER_LEFT_46_TM=60.870
PRIMER_RIGHT_46_TM=59.969
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=714
PRIMER_PAIR_47_PENALTY=1.933488
PRIMER_LEFT_47_PENALTY=1.432264
PRIMER_RIGHT_47_PENALTY=0.501224
PRIMER_LEFT_47_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_47_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_47=300,20
PRIMER_RIGHT_47=1173,20
PRIMER_LEFT_47_TM=58.568
PRIMER_RIGHT_47_TM=59.499
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=874
PRIMER_PAIR_48_PENALTY=1.942595
PRIMER_LEFT_48_PENALTY=1.432264
PRIMER_RIGHT_48_PENALTY=0.510331
PRIMER_LEFT_48_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_48_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_48=300,20
PRIMER_RIGHT_48=1227,20
PRIMER_LEFT_48_TM=58.568
PRIMER_RIGHT_48_TM=60.510
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=928
PRIMER_PAIR_49_PENALTY=1.942595
PRIMER_LEFT_49_PENALTY=1.432264
PRIMER_RIGHT_49_PENALTY=0.510331
PRIMER_LEFT_49_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_49_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_49=300,20
PRIMER_RIGHT_49=1228,20
PRIMER_LEFT_49_TM=58.568
PRIMER_RIGHT_49_TM=60.510
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=929
=
SEQUENCE_ID=MAGIC.338_2_6350292_6350912_po-0_ler-0_63352_43957_TRUE
SEQUENCE_TARGET=950,720
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAAGGNTNCTCATCNAAACTNGATCANANTTTTTNTTTNTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAGCNTNTTTNTGCATCAAACNCATATNNGGGGGGGTNAANNTTAGGCTATCAACAATNGTTTTTCNNTTCAACACCNTCCACATAAGCTTCTCTCTCTTCCACATNANTTTNTCTNTCTTCTACATAATAATTCAATACCCACTCAAATTTTATACTNTACANTGNTTTGTTTAATAAAATTCAANACCCTACCCCANAANTTNTATTTCATTATTTTTNTTTTNTTTTATNTNTTAAATNNATAATTAATNNANTTTTNGGNGTTAAANGANTTATTATTGNGATNNATTTCACTNGNAAAAAANAGAAAAATATNTAGATANNNNNNNNNNNNCTAATNAANNTTTTTTAGTGAAAATAGATGTNNNTTTTTNTGTGTCCAAATCAAATGAAANNNNNNNNNNNNNNNAGAGTTTAAAAAATGATGAATTTTANTNNAAAAATAATTTAAAACAAATAAATATTTTATAAGATAATNGACNTNCAATAATTCGGAGGAAAANAAAATATAANAAATTTCAGTANCAAATAAAAGAANAACACATGTCCTATCTTTATTTAACTCTNCCCCACAATTTNTNTCTCCAATTATTTNATGACAAGTNNCNATNANGGNTNNATTTTTAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAATNNACATANGATTCATTTCTTTATTNAATANCCCATTGTAANNATTCAACTATTGAATAANTTTTTNCNNNNNNNNNNTGTAGATAGNNATAGNGANNTCAACCAAATGANCCANTTGCTNAAAACAAATATTTCACAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_5359069_5359125_ct-1_ler-0_11242_113518_TRUE
SEQUENCE_TARGET=950,156
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_5182969_5183344_po-0_bur-0_391138_2824_TRUE
SEQUENCE_TARGET=950,475
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGATTTATCAANAATTNNTNGGANTTTTTATTTTTATACCATCATAATATATTTATTTAAATTAGTAATANAAGGCATTTTATTTACTATAAAANNTNTTTTATTAAAACTATTATATTTACTNATCCATTTTNTCTAAATATTNAAGGTNAAAAACTTACAACAATAATAAGTTACTNCTACANCAAANACAANTACAACAAAATNTCTAGAGCAAAANTTATATAGTTANAGCTCAACCAATNACTCNCATANAANNCANNNNNNCATTGGAAACTAACAAATGATACNNNNNNTTAGANGAACTTTAAATTTCATATTTTCAATAAAATGAGAGAGTTTGTAAAAGAATATACAATGATTTGTTTAACCAGCATTAGAGGTTTTTGAAGTTTTTCATNGCCAACTAAAAAGAAAAGGGTAAACATATGTTNAAGAACCTTAAAGAATNTTTGACNTGGAGTCATCCAGAGAGACATGAGGTCGAAGAAGCTTTTCGAATATATTTGGGAGAGNATCACGTCACGGCGGTTGCTCATGAAATAAACGTCCGGTGGTGCAAAAAATNTAGTGACGAATCACATATACTGTAGCTGNCTGAAGCAAAATGTATATGGTNNGAGCGACTAGTNNNATCATCTAATGTACAAGCTTCATCTTNGTCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATGGATCAAAGTTCAAACACNCTTAGAATTCATACCATCAATTTTCTAGACCTGGCAAGTGATNACTTCCTAGNCCTCACAAACNTAATTGTTGCGCNNNCNATAGTTCANAAGCTTGTGGCATNAANAGCTAAANNATCCAACTCATTCCAGATTCTTTTGCCCCTAGCTTCTTAGCAGNTTCTCTCACTTNACTTAACTGATCCCACATGCCTAACGTAGCATAAGCATTCGAAAGAGATGTATANACCGATGGATCATCCGGAAACNCCTTGAGTAATTTCTCAGCAANTACAGTAACCAGTCCGGCATTCTTGTGAGTTCCGCAAGCAGTGAGCAGAGACGTCCATATTGTTTTGTCGGGNTCCAACGGCATTTTNTTTATCAATTCNTNNGCNTCTTGGAACANCCCNGCTTGACCAAGAAGATCAACTACACAATAATAATGCTCNAGATACGGTTCAAGACCATATTCAGNTTTCATCGNTTCNAGGNTTGATCTTGCTTCTTCAAGNAGTCNAGAATGTCGGCAAGCNGAAAGAANCCCCAAAAAAGTTACCTNATTTGGTTCAANTCCANNANTNATCATCTTGTGGAAATACNGAAACGCTTCTTCGACTCGTCCGTTTTGTCCNAATCCAACAATTATCCCTGTCCAAGA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_13991146_13991490_sf-2_rsch-4_19595_35185_TRUE
SEQUENCE_TARGET=950,444
SEQUENCE_TEMPLATE=AAGGGGATCAACAAATTNGAGNGCNATCTTCCTTAATCACGAACCAATCTGATAACCCNTGAAGATTATCGAGAGNTTGGAAAGAGAAGAGAAAAGAGGAAAGGGGAGAGAAGAGATGATTAGATCTGAAATTGAATCTGTCGATAATGAACCCTNGAATTCGGCAGTNTTANATTTTAGTTTTAGAGTATATTACTATACATAACATTATAGTAGTTAGCCATTNAAAATTCGTACATTATANTAGCTACCAATTAGGCACATACAACATAGNAATGCAATAGATTTGTAACTATAATTAGCTACTAATGTTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTGTGTGATTTTGTGCAGTTTCCATCTCTTAAAACAATGAGCAAATGATTAAAAAATGNNAAAAATACAAAAAAATNATATCCNAAAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_12762147_12762472_oy-0_ct-1_207111_9576_TRUE
SEQUENCE_TARGET=950,425
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_10475095_10475839_ct-1_oy-0_178992_4643_TRUE
SEQUENCE_TARGET=950,844
SEQUENCE_TEMPLATE=TGTTGCCANTTTTNAAGTNTTTTANTTANATGTGTAACGCGTCTACTATAATCTTTCTNGATTCTCCTNCTTTTCACANCTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATNATTNACCAATAAAAAGGAAAGATCACNANTCANNNNNNNNNNNNNNNTGATGATACATTGATAGNTGAATNTATGCAGAGTACNGANTGATGAGNTCCNGTGGANATCCGATAGAGATNTTTTANTATGGACTAATCAAAANTTAAAAAANAAATATGAAANCATANTTTATGTTGGGCTTAGGCCCAAGCTGTTTGTATNATCATCATAAAACCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGTNATAAATATGAAAACNTTTTGACAANNGNANNNTANTTNCTNNANTNATATGGGATCNTGTTTTCAAAAAAANAAANAAAANAAAANAANNNNNNATATGGGATCCAATAAATGTTATTTGNAGTCANNNNNNNNNCNNNNATATAAAAGTTTGATATTTGTNTATCTTGNGTNCTCAAAAANATACTGATGGGTTTAANNACATNAATTAGCTTTTCTATTCAAGACATTTTGGTAAAAGTGTGATNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNGTANTTTAGTGAGATCAAGAGCATAATTAAAATATTGAAGANTTTACAAATGCNTTATCTCNTCAAAAGGAAGNATTATAANTATGANCAAACCCNNGNCTNNNNAATTTCAGACTCAAGCTAAGNTTTTTTTGTTTAAATTTTACTTGATTCTCTCAANGATGNNAAAAAAAAACTACATAGGAGTTTGAAACAAAGCATACGNAAGGAAGAATAAGAANTCAATTCCCTTCGTTCCGCCGGTTAAAAGANCAGTCATGCTTTGAAGATTAATCCACTAGTTAATCATTAATTGTTGTGTGACACTAACTAAAACTAAAGATAGAAGCTAGAACAATTTAGAAAGAACCAGTCNTATCTAAAAATANAGAGTGATAGATTAATGATGGTTGATTAGTAGTCCAATATGGTAGCGTTTTTTGGACTTAAGCGAGTGTTAGCTATGTGCTGCATTCTAGCTTTTAATTTCTCATCATTGATGTCAAAATTGAATAAATGGGCATTATCCATGTAAGNGTGGTTTTAATTCGGAATATTTTTACCTAAATCGAAAAANNCGATTGTCNAACCGAACCAAATTACCTAAANCCTAAACNTCTATTTCAAGTCGTTTCAGGACTCTTTTANCCAAANCGAGTAATCCNAATTACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14494181_14494425_po-0_rsch-4_213902_44198_TRUE
SEQUENCE_TARGET=950,344
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTTTGACNNGTGTGCATGCAGTCTCNGGTGCTGTNGGATCGGTCCACCGNNNNNNNNGATTGTTNTACTAGAANGCCCGNGATGCTNGGTCTCNAATCCTAGAAAATTGAAGATAATTGATCGNCAANCCAAAGATCCTCACATATGAATGGAGGAGTCTCCCCTNGAAGAACCCCATTCTTACACATCAGAANAGGAGACTTAAGAATTCGAATNAGTTCNTCCATTATCTATNTAATTNCCTTTCTCTTGTAATTNNCGAGCCAAAAGTTAAATAAAANNAGGTTAGATCCNTTTCTCTCNTNACTTGGATTTTACCAAAATNNGTTTAGGNTTAGATTGANCNGACTGNACAACAATTAAACAGGTTANGTGNTTAGCGCCTAACANTTTTTTTGAAAACTGCATTTAGTATATTNTATTTTAAANCCAANNANANTTAAAATTGCTTGCCAATNCACAAAANTTTATTTTAAGTAAGGTTTTGACATTTATTTGTAAAACATATTTTGCACNTTTGCAATCAATTTGTNAAATNAANNTTTGAGTTAACNAAAAACNTTGNATATNNNATATGGATCTTCNNANGATTGTAACAATGATAAGTAAGAGTATCATCAACCTTTTATTAACGAGTAGGAGAAAGACGACAANCAAAATGTANCTTCAANGNGNTATTTNATCCACNTACTNATTCAAGATGGCAATGNTATTTGTCTTATTTCTTGCCATGTAAAACAGACTGATGTNATCCNGNGGCTGTNTTATGCAGAAAATAGGATNTNAGGACCATTACAATCNTAAATCTAACNAAANNNNTATAAACATTTAAAATGAAAAAGTTTAAAAGCTAAACNTGTTAAGTGTNTNAACGAGAACAATCGCTAGAGAAACATAGTAAAATTTGGAGTTGCGNNNNNAGAGAAGAGGATATNTTATTTANAAAAATATATNTATNTATANATATATATATATATATATATATATATATATATATATATNNNNNNNNNNAACTTTTGTTGAAAATTCGTCAAAATCACAGTTTGGTAGGAGTTCAANTTTTTTNTNGTTTCTNTGCTGAAATTCTAGGAGTGGCATTTTAACCAAACCAAACCCAAGCTTAGACTTTAACCATTCGGTTATGTCTCTTCTCAACCCAAATGGTTCGGTTTGGTTAGGTTTGACAACCCAATATATTTATTTAATTAATAATATTGTATATTATTAAATATGTAATTANNNNCANTTNTTTTTCATGGTTTATTTGANTTANTAAACTTTTTGCTTAAGCTAACTTATATCAAATTTAAAGTTCAAACGATAATGAACAAAATAATTCTGTTTCATTACATTGATTAGTCTTCTAAAACTAAACTAAAACCAAACCAAACTAAAGTCAAATCAAACCAAACTGAACCGAACATAGATTGACCCAAATCAAACCAAACTGAACCGAACATAGATTGACCCAAACCCTAACCAAACCNNATNCTNGATCGGCTGCGGCATAAANANTGGTGATGATGTTTTATTCTATGATTTATACACTAACCGATGTCAAGTTGGACTTTTCCCTGATGTAGAACNAGATTGGATTTTCAGAAGAACATAAACTTCTTAACNACACATCTAGCTTGGTTGGTATACCGNATCTCTANATTACAATTTATTTCCCTTAGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.595489
PRIMER_LEFT_0_PENALTY=3.563720
PRIMER_RIGHT_0_PENALTY=0.031769
PRIMER_LEFT_0_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_0_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_0=733,22
PRIMER_RIGHT_0=1686,20
PRIMER_LEFT_0_TM=58.436
PRIMER_RIGHT_0_TM=59.968
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=954
PRIMER_PAIR_1_PENALTY=3.595489
PRIMER_LEFT_1_PENALTY=3.563720
PRIMER_RIGHT_1_PENALTY=0.031769
PRIMER_LEFT_1_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_1_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_1=732,22
PRIMER_RIGHT_1=1686,20
PRIMER_LEFT_1_TM=58.436
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=955
PRIMER_PAIR_2_PENALTY=3.629697
PRIMER_LEFT_2_PENALTY=3.597928
PRIMER_RIGHT_2_PENALTY=0.031769
PRIMER_LEFT_2_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_2=732,23
PRIMER_RIGHT_2=1686,20
PRIMER_LEFT_2_TM=59.402
PRIMER_RIGHT_2_TM=59.968
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=955
PRIMER_PAIR_3_PENALTY=3.655097
PRIMER_LEFT_3_PENALTY=3.623327
PRIMER_RIGHT_3_PENALTY=0.031769
PRIMER_LEFT_3_SEQUENCE=GAACCCCATTCTTACACATCA
PRIMER_RIGHT_3_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_3=733,21
PRIMER_RIGHT_3=1686,20
PRIMER_LEFT_3_TM=57.377
PRIMER_RIGHT_3_TM=59.968
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=954
PRIMER_PAIR_4_PENALTY=4.148566
PRIMER_LEFT_4_PENALTY=4.116797
PRIMER_RIGHT_4_PENALTY=0.031769
PRIMER_LEFT_4_SEQUENCE=GAAGAACCCCATTCTTACACATC
PRIMER_RIGHT_4_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_4=730,23
PRIMER_RIGHT_4=1686,20
PRIMER_LEFT_4_TM=58.883
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=957
PRIMER_PAIR_5_PENALTY=4.316844
PRIMER_LEFT_5_PENALTY=3.563720
PRIMER_RIGHT_5_PENALTY=0.753123
PRIMER_LEFT_5_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_5_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_5=733,22
PRIMER_RIGHT_5=1684,20
PRIMER_LEFT_5_TM=58.436
PRIMER_RIGHT_5_TM=60.753
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=952
PRIMER_PAIR_6_PENALTY=4.316844
PRIMER_LEFT_6_PENALTY=3.563720
PRIMER_RIGHT_6_PENALTY=0.753123
PRIMER_LEFT_6_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_6_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_6=733,22
PRIMER_RIGHT_6=1685,20
PRIMER_LEFT_6_TM=58.436
PRIMER_RIGHT_6_TM=60.753
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=953
PRIMER_PAIR_7_PENALTY=4.316844
PRIMER_LEFT_7_PENALTY=3.563720
PRIMER_RIGHT_7_PENALTY=0.753123
PRIMER_LEFT_7_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_7_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_7=732,22
PRIMER_RIGHT_7=1684,20
PRIMER_LEFT_7_TM=58.436
PRIMER_RIGHT_7_TM=60.753
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=953
PRIMER_PAIR_8_PENALTY=4.316844
PRIMER_LEFT_8_PENALTY=3.563720
PRIMER_RIGHT_8_PENALTY=0.753123
PRIMER_LEFT_8_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_8_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_8=732,22
PRIMER_RIGHT_8=1685,20
PRIMER_LEFT_8_TM=58.436
PRIMER_RIGHT_8_TM=60.753
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=954
PRIMER_PAIR_9_PENALTY=4.351051
PRIMER_LEFT_9_PENALTY=3.597928
PRIMER_RIGHT_9_PENALTY=0.753123
PRIMER_LEFT_9_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_9_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_9=732,23
PRIMER_RIGHT_9=1684,20
PRIMER_LEFT_9_TM=59.402
PRIMER_RIGHT_9_TM=60.753
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=953
PRIMER_PAIR_10_PENALTY=4.351051
PRIMER_LEFT_10_PENALTY=3.597928
PRIMER_RIGHT_10_PENALTY=0.753123
PRIMER_LEFT_10_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_10_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_10=732,23
PRIMER_RIGHT_10=1685,20
PRIMER_LEFT_10_TM=59.402
PRIMER_RIGHT_10_TM=60.753
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=954
PRIMER_PAIR_11_PENALTY=4.376451
PRIMER_LEFT_11_PENALTY=3.623327
PRIMER_RIGHT_11_PENALTY=0.753123
PRIMER_LEFT_11_SEQUENCE=GAACCCCATTCTTACACATCA
PRIMER_RIGHT_11_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_11=733,21
PRIMER_RIGHT_11=1684,20
PRIMER_LEFT_11_TM=57.377
PRIMER_RIGHT_11_TM=60.753
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=952
PRIMER_PAIR_12_PENALTY=4.376451
PRIMER_LEFT_12_PENALTY=3.623327
PRIMER_RIGHT_12_PENALTY=0.753123
PRIMER_LEFT_12_SEQUENCE=GAACCCCATTCTTACACATCA
PRIMER_RIGHT_12_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_12=733,21
PRIMER_RIGHT_12=1685,20
PRIMER_LEFT_12_TM=57.377
PRIMER_RIGHT_12_TM=60.753
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=953
PRIMER_PAIR_13_PENALTY=4.381707
PRIMER_LEFT_13_PENALTY=4.349938
PRIMER_RIGHT_13_PENALTY=0.031769
PRIMER_LEFT_13_SEQUENCE=GAAGAACCCCATTCTTACACA
PRIMER_RIGHT_13_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_13=730,21
PRIMER_RIGHT_13=1686,20
PRIMER_LEFT_13_TM=56.650
PRIMER_RIGHT_13_TM=59.968
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.8000
PRIMER_PAIR_13_COMPL_ANY=7.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=957
PRIMER_PAIR_14_PENALTY=4.426771
PRIMER_LEFT_14_PENALTY=3.563720
PRIMER_RIGHT_14_PENALTY=0.863051
PRIMER_LEFT_14_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_14_SEQUENCE=CGAACCATTTGGGTTGAGAA
PRIMER_LEFT_14=733,22
PRIMER_RIGHT_14=1731,20
PRIMER_LEFT_14_TM=58.436
PRIMER_RIGHT_14_TM=60.863
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=999
PRIMER_PAIR_15_PENALTY=4.426771
PRIMER_LEFT_15_PENALTY=3.563720
PRIMER_RIGHT_15_PENALTY=0.863051
PRIMER_LEFT_15_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_15_SEQUENCE=CGAACCATTTGGGTTGAGAA
PRIMER_LEFT_15=732,22
PRIMER_RIGHT_15=1731,20
PRIMER_LEFT_15_TM=58.436
PRIMER_RIGHT_15_TM=60.863
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=1000
PRIMER_PAIR_16_PENALTY=4.460979
PRIMER_LEFT_16_PENALTY=3.597928
PRIMER_RIGHT_16_PENALTY=0.863051
PRIMER_LEFT_16_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_16_SEQUENCE=CGAACCATTTGGGTTGAGAA
PRIMER_LEFT_16=732,23
PRIMER_RIGHT_16=1731,20
PRIMER_LEFT_16_TM=59.402
PRIMER_RIGHT_16_TM=60.863
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=1000
PRIMER_PAIR_17_PENALTY=4.486379
PRIMER_LEFT_17_PENALTY=3.623327
PRIMER_RIGHT_17_PENALTY=0.863051
PRIMER_LEFT_17_SEQUENCE=GAACCCCATTCTTACACATCA
PRIMER_RIGHT_17_SEQUENCE=CGAACCATTTGGGTTGAGAA
PRIMER_LEFT_17=733,21
PRIMER_RIGHT_17=1731,20
PRIMER_LEFT_17_TM=57.377
PRIMER_RIGHT_17_TM=60.863
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=999
PRIMER_PAIR_18_PENALTY=4.505737
PRIMER_LEFT_18_PENALTY=4.473968
PRIMER_RIGHT_18_PENALTY=0.031769
PRIMER_LEFT_18_SEQUENCE=AACCCCATTCTTACACATCAG
PRIMER_RIGHT_18_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_18=734,21
PRIMER_RIGHT_18=1686,20
PRIMER_LEFT_18_TM=56.526
PRIMER_RIGHT_18_TM=59.968
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=8.8000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=953
PRIMER_PAIR_19_PENALTY=4.649614
PRIMER_LEFT_19_PENALTY=4.617845
PRIMER_RIGHT_19_PENALTY=0.031769
PRIMER_LEFT_19_SEQUENCE=AAGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_19_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_19=731,24
PRIMER_RIGHT_19=1686,20
PRIMER_LEFT_19_TM=60.618
PRIMER_RIGHT_19_TM=59.968
PRIMER_LEFT_19_GC_PERCENT=41.667
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=8.8000
PRIMER_PAIR_19_COMPL_ANY=7.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=956
PRIMER_PAIR_20_PENALTY=4.695631
PRIMER_LEFT_20_PENALTY=4.663862
PRIMER_RIGHT_20_PENALTY=0.031769
PRIMER_LEFT_20_SEQUENCE=AACCCCATTCTTACACATCA
PRIMER_RIGHT_20_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_20=734,20
PRIMER_RIGHT_20=1686,20
PRIMER_LEFT_20_TM=55.336
PRIMER_RIGHT_20_TM=59.968
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=8.8000
PRIMER_PAIR_20_COMPL_ANY=7.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=953
PRIMER_PAIR_21_PENALTY=4.869921
PRIMER_LEFT_21_PENALTY=4.116797
PRIMER_RIGHT_21_PENALTY=0.753123
PRIMER_LEFT_21_SEQUENCE=GAAGAACCCCATTCTTACACATC
PRIMER_RIGHT_21_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_21=730,23
PRIMER_RIGHT_21=1684,20
PRIMER_LEFT_21_TM=58.883
PRIMER_RIGHT_21_TM=60.753
PRIMER_LEFT_21_GC_PERCENT=43.478
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.3000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=955
PRIMER_PAIR_22_PENALTY=4.869921
PRIMER_LEFT_22_PENALTY=4.116797
PRIMER_RIGHT_22_PENALTY=0.753123
PRIMER_LEFT_22_SEQUENCE=GAAGAACCCCATTCTTACACATC
PRIMER_RIGHT_22_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_22=730,23
PRIMER_RIGHT_22=1685,20
PRIMER_LEFT_22_TM=58.883
PRIMER_RIGHT_22_TM=60.753
PRIMER_LEFT_22_GC_PERCENT=43.478
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.3000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=956
PRIMER_PAIR_23_PENALTY=4.943580
PRIMER_LEFT_23_PENALTY=4.911811
PRIMER_RIGHT_23_PENALTY=0.031769
PRIMER_LEFT_23_SEQUENCE=AAGAACCCCATTCTTACACATC
PRIMER_RIGHT_23_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_23=731,22
PRIMER_RIGHT_23=1686,20
PRIMER_LEFT_23_TM=57.088
PRIMER_RIGHT_23_TM=59.968
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.3000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=7.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=956
PRIMER_PAIR_24_PENALTY=5.103062
PRIMER_LEFT_24_PENALTY=4.349938
PRIMER_RIGHT_24_PENALTY=0.753123
PRIMER_LEFT_24_SEQUENCE=GAAGAACCCCATTCTTACACA
PRIMER_RIGHT_24_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_24=730,21
PRIMER_RIGHT_24=1684,20
PRIMER_LEFT_24_TM=56.650
PRIMER_RIGHT_24_TM=60.753
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=955
PRIMER_PAIR_25_PENALTY=5.103062
PRIMER_LEFT_25_PENALTY=4.349938
PRIMER_RIGHT_25_PENALTY=0.753123
PRIMER_LEFT_25_SEQUENCE=GAAGAACCCCATTCTTACACA
PRIMER_RIGHT_25_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_25=730,21
PRIMER_RIGHT_25=1685,20
PRIMER_LEFT_25_TM=56.650
PRIMER_RIGHT_25_TM=60.753
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=956
PRIMER_PAIR_26_PENALTY=5.176702
PRIMER_LEFT_26_PENALTY=3.563720
PRIMER_RIGHT_26_PENALTY=1.612981
PRIMER_LEFT_26_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_26_SEQUENCE=TGGTTTGGTTAAAATGCCAC
PRIMER_LEFT_26=733,22
PRIMER_RIGHT_26=1674,20
PRIMER_LEFT_26_TM=58.436
PRIMER_RIGHT_26_TM=58.387
PRIMER_LEFT_26_GC_PERCENT=45.455
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=9.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=942
PRIMER_PAIR_27_PENALTY=5.176702
PRIMER_LEFT_27_PENALTY=3.563720
PRIMER_RIGHT_27_PENALTY=1.612981
PRIMER_LEFT_27_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_27_SEQUENCE=TGGTTTGGTTAAAATGCCAC
PRIMER_LEFT_27=732,22
PRIMER_RIGHT_27=1674,20
PRIMER_LEFT_27_TM=58.436
PRIMER_RIGHT_27_TM=58.387
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=943
PRIMER_PAIR_28_PENALTY=5.210909
PRIMER_LEFT_28_PENALTY=3.597928
PRIMER_RIGHT_28_PENALTY=1.612981
PRIMER_LEFT_28_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_28_SEQUENCE=TGGTTTGGTTAAAATGCCAC
PRIMER_LEFT_28=732,23
PRIMER_RIGHT_28=1674,20
PRIMER_LEFT_28_TM=59.402
PRIMER_RIGHT_28_TM=58.387
PRIMER_LEFT_28_GC_PERCENT=43.478
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=943
PRIMER_PAIR_29_PENALTY=5.227091
PRIMER_LEFT_29_PENALTY=4.473968
PRIMER_RIGHT_29_PENALTY=0.753123
PRIMER_LEFT_29_SEQUENCE=AACCCCATTCTTACACATCAG
PRIMER_RIGHT_29_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_29=734,21
PRIMER_RIGHT_29=1684,20
PRIMER_LEFT_29_TM=56.526
PRIMER_RIGHT_29_TM=60.753
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=951
PRIMER_PAIR_30_PENALTY=5.227091
PRIMER_LEFT_30_PENALTY=4.473968
PRIMER_RIGHT_30_PENALTY=0.753123
PRIMER_LEFT_30_SEQUENCE=AACCCCATTCTTACACATCAG
PRIMER_RIGHT_30_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_30=734,21
PRIMER_RIGHT_30=1685,20
PRIMER_LEFT_30_TM=56.526
PRIMER_RIGHT_30_TM=60.753
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=952
PRIMER_PAIR_31_PENALTY=5.236309
PRIMER_LEFT_31_PENALTY=3.623327
PRIMER_RIGHT_31_PENALTY=1.612981
PRIMER_LEFT_31_SEQUENCE=GAACCCCATTCTTACACATCA
PRIMER_RIGHT_31_SEQUENCE=TGGTTTGGTTAAAATGCCAC
PRIMER_LEFT_31=733,21
PRIMER_RIGHT_31=1674,20
PRIMER_LEFT_31_TM=57.377
PRIMER_RIGHT_31_TM=58.387
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=942
PRIMER_PAIR_32_PENALTY=5.300034
PRIMER_LEFT_32_PENALTY=3.563720
PRIMER_RIGHT_32_PENALTY=1.736313
PRIMER_LEFT_32_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_32_SEQUENCE=TAAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_32=733,22
PRIMER_RIGHT_32=1686,21
PRIMER_LEFT_32_TM=58.436
PRIMER_RIGHT_32_TM=60.736
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=954
PRIMER_PAIR_33_PENALTY=5.300034
PRIMER_LEFT_33_PENALTY=3.563720
PRIMER_RIGHT_33_PENALTY=1.736313
PRIMER_LEFT_33_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_33_SEQUENCE=TAAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_33=732,22
PRIMER_RIGHT_33=1686,21
PRIMER_LEFT_33_TM=58.436
PRIMER_RIGHT_33_TM=60.736
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=955
PRIMER_PAIR_34_PENALTY=5.310195
PRIMER_LEFT_34_PENALTY=3.563720
PRIMER_RIGHT_34_PENALTY=1.746475
PRIMER_LEFT_34_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_34_SEQUENCE=TCTAAGCTTGGGTTTGGTTTG
PRIMER_LEFT_34=733,22
PRIMER_RIGHT_34=1688,21
PRIMER_LEFT_34_TM=58.436
PRIMER_RIGHT_34_TM=59.254
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.6000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=7.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=956
PRIMER_PAIR_35_PENALTY=5.310195
PRIMER_LEFT_35_PENALTY=3.563720
PRIMER_RIGHT_35_PENALTY=1.746475
PRIMER_LEFT_35_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_35_SEQUENCE=TCTAAGCTTGGGTTTGGTTTG
PRIMER_LEFT_35=732,22
PRIMER_RIGHT_35=1688,21
PRIMER_LEFT_35_TM=58.436
PRIMER_RIGHT_35_TM=59.254
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=7.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=957
PRIMER_PAIR_36_PENALTY=5.334242
PRIMER_LEFT_36_PENALTY=3.597928
PRIMER_RIGHT_36_PENALTY=1.736313
PRIMER_LEFT_36_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_36_SEQUENCE=TAAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_36=732,23
PRIMER_RIGHT_36=1686,21
PRIMER_LEFT_36_TM=59.402
PRIMER_RIGHT_36_TM=60.736
PRIMER_LEFT_36_GC_PERCENT=43.478
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=7.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=955
PRIMER_PAIR_37_PENALTY=5.337019
PRIMER_LEFT_37_PENALTY=4.473968
PRIMER_RIGHT_37_PENALTY=0.863051
PRIMER_LEFT_37_SEQUENCE=AACCCCATTCTTACACATCAG
PRIMER_RIGHT_37_SEQUENCE=CGAACCATTTGGGTTGAGAA
PRIMER_LEFT_37=734,21
PRIMER_RIGHT_37=1731,20
PRIMER_LEFT_37_TM=56.526
PRIMER_RIGHT_37_TM=60.863
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=998
PRIMER_PAIR_38_PENALTY=5.344403
PRIMER_LEFT_38_PENALTY=3.597928
PRIMER_RIGHT_38_PENALTY=1.746475
PRIMER_LEFT_38_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_38_SEQUENCE=TCTAAGCTTGGGTTTGGTTTG
PRIMER_LEFT_38=732,23
PRIMER_RIGHT_38=1688,21
PRIMER_LEFT_38_TM=59.402
PRIMER_RIGHT_38_TM=59.254
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=7.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=957
PRIMER_PAIR_39_PENALTY=5.359641
PRIMER_LEFT_39_PENALTY=3.623327
PRIMER_RIGHT_39_PENALTY=1.736313
PRIMER_LEFT_39_SEQUENCE=GAACCCCATTCTTACACATCA
PRIMER_RIGHT_39_SEQUENCE=TAAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_39=733,21
PRIMER_RIGHT_39=1686,21
PRIMER_LEFT_39_TM=57.377
PRIMER_RIGHT_39_TM=60.736
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=7.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=954
PRIMER_PAIR_40_PENALTY=5.369803
PRIMER_LEFT_40_PENALTY=3.623327
PRIMER_RIGHT_40_PENALTY=1.746475
PRIMER_LEFT_40_SEQUENCE=GAACCCCATTCTTACACATCA
PRIMER_RIGHT_40_SEQUENCE=TCTAAGCTTGGGTTTGGTTTG
PRIMER_LEFT_40=733,21
PRIMER_RIGHT_40=1688,21
PRIMER_LEFT_40_TM=57.377
PRIMER_RIGHT_40_TM=59.254
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=956
PRIMER_PAIR_41_PENALTY=5.370968
PRIMER_LEFT_41_PENALTY=4.617845
PRIMER_RIGHT_41_PENALTY=0.753123
PRIMER_LEFT_41_SEQUENCE=AAGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_41_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_41=731,24
PRIMER_RIGHT_41=1684,20
PRIMER_LEFT_41_TM=60.618
PRIMER_RIGHT_41_TM=60.753
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.6000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=954
PRIMER_PAIR_42_PENALTY=5.370968
PRIMER_LEFT_42_PENALTY=4.617845
PRIMER_RIGHT_42_PENALTY=0.753123
PRIMER_LEFT_42_SEQUENCE=AAGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_42_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_42=731,24
PRIMER_RIGHT_42=1685,20
PRIMER_LEFT_42_TM=60.618
PRIMER_RIGHT_42_TM=60.753
PRIMER_LEFT_42_GC_PERCENT=41.667
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=955
PRIMER_PAIR_43_PENALTY=5.416986
PRIMER_LEFT_43_PENALTY=4.663862
PRIMER_RIGHT_43_PENALTY=0.753123
PRIMER_LEFT_43_SEQUENCE=AACCCCATTCTTACACATCA
PRIMER_RIGHT_43_SEQUENCE=AGCTTGGGTTTGGTTTGGTT
PRIMER_LEFT_43=734,20
PRIMER_RIGHT_43=1684,20
PRIMER_LEFT_43_TM=55.336
PRIMER_RIGHT_43_TM=60.753
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=951
PRIMER_PAIR_44_PENALTY=5.416986
PRIMER_LEFT_44_PENALTY=4.663862
PRIMER_RIGHT_44_PENALTY=0.753123
PRIMER_LEFT_44_SEQUENCE=AACCCCATTCTTACACATCA
PRIMER_RIGHT_44_SEQUENCE=AAGCTTGGGTTTGGTTTGGT
PRIMER_LEFT_44=734,20
PRIMER_RIGHT_44=1685,20
PRIMER_LEFT_44_TM=55.336
PRIMER_RIGHT_44_TM=60.753
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=952
PRIMER_PAIR_45_PENALTY=5.417116
PRIMER_LEFT_45_PENALTY=3.563720
PRIMER_RIGHT_45_PENALTY=1.853395
PRIMER_LEFT_45_SEQUENCE=GAACCCCATTCTTACACATCAG
PRIMER_RIGHT_45_SEQUENCE=CTAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_45=733,22
PRIMER_RIGHT_45=1687,21
PRIMER_LEFT_45_TM=58.436
PRIMER_RIGHT_45_TM=60.853
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.6000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=955
PRIMER_PAIR_46_PENALTY=5.417116
PRIMER_LEFT_46_PENALTY=3.563720
PRIMER_RIGHT_46_PENALTY=1.853395
PRIMER_LEFT_46_SEQUENCE=AGAACCCCATTCTTACACATCA
PRIMER_RIGHT_46_SEQUENCE=CTAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_46=732,22
PRIMER_RIGHT_46=1687,21
PRIMER_LEFT_46_TM=58.436
PRIMER_RIGHT_46_TM=60.853
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.8000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=956
PRIMER_PAIR_47_PENALTY=5.447513
PRIMER_LEFT_47_PENALTY=5.415744
PRIMER_RIGHT_47_PENALTY=0.031769
PRIMER_LEFT_47_SEQUENCE=AGAACCCCATTCTTACACATC
PRIMER_RIGHT_47_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_47=732,21
PRIMER_RIGHT_47=1686,20
PRIMER_LEFT_47_TM=55.584
PRIMER_RIGHT_47_TM=59.968
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.3000
PRIMER_RIGHT_47_END_STABILITY=8.8000
PRIMER_PAIR_47_COMPL_ANY=7.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=955
PRIMER_PAIR_48_PENALTY=5.451323
PRIMER_LEFT_48_PENALTY=3.597928
PRIMER_RIGHT_48_PENALTY=1.853395
PRIMER_LEFT_48_SEQUENCE=AGAACCCCATTCTTACACATCAG
PRIMER_RIGHT_48_SEQUENCE=CTAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_48=732,23
PRIMER_RIGHT_48=1687,21
PRIMER_LEFT_48_TM=59.402
PRIMER_RIGHT_48_TM=60.853
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=8.8000
PRIMER_PAIR_48_COMPL_ANY=7.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=956
PRIMER_PAIR_49_PENALTY=5.459720
PRIMER_LEFT_49_PENALTY=5.427951
PRIMER_RIGHT_49_PENALTY=0.031769
PRIMER_LEFT_49_SEQUENCE=GAAGAACCCCATTCTTACACATCA
PRIMER_RIGHT_49_SEQUENCE=TAAGCTTGGGTTTGGTTTGG
PRIMER_LEFT_49=730,24
PRIMER_RIGHT_49=1686,20
PRIMER_LEFT_49_TM=61.428
PRIMER_RIGHT_49_TM=59.968
PRIMER_LEFT_49_GC_PERCENT=41.667
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=8.8000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=957
=
SEQUENCE_ID=MAGIC.446_3_6652377_6652560_tsu-0_col-0_34356_44890_TRUE
SEQUENCE_TARGET=950,283
SEQUENCE_TEMPLATE=ACATAGGGTTAAGAAATTTTTTAATCATGCATACATTGTTATAGACAAACAGAAGAAGCAAACGAGCAGACAAAAGACTGTAGCACCAAAAGAGCCATGTGAAGCTGAGCTGTAAATGTTCAGCTGATCAAAAGCCCCAAAACAGAAAACAATTATAAAAAATAAAAACATAAAATTATAAAATCTATCTTTCTGGATTCAGATTTTAGGGTTTGACTAAAACTCTGCTAACGTTTGTCTTCTTGTGGTACTCTNACTCACGTGCTTCACTTCTAGTCCTAATGGAATTTTCCCACGTTTCTCCAACTAGGGTTTTGACGCGTGGTACTTTNATACTTCTACGTAAGTGGACATANAAGTTGTGTACAAAACAATATTTTCGGAATGTGCAAAAANCNTATGTTTCTTGAGTGCAATTGCANTTNTTTTTTCTTAGGTTCAGATCCTGAAAGTTTTTCAGATCGAGTTTCTCTAGTGAATACGTGTTTAGTTCCNATTTTTTTACGAGAAGAGGTNATNTTANCAATCAAATACATTATAACANCTTACAGTTTTTNTNTATATTAAAGAAGCAAGGAGCTAAAACTAGAGTAGGATTTACATTNTTNAAAAAGTTGTAAAGCATAAGGGAATCTAGACATATAGATAATATCAGAGGACTTTTGTGGTGGATAAGTAAGTTGTATCTAAGTTTTATTTTGGCTGTCTTCTTGTCCCCAATTTTCTCTACATCTTCTGTCTTCTTCCTCTTACTTAACTCTCTCTTTCATTTTTGTTATTTATTTAACAATAAATTACCTTTTCACCTCACTAATCTGATTTCTTAACACTTAAGTCCTCCACCAGCAAAAAAGANAAACTAACACGTNCAATTATTGTTGNATTTTTACTNNTATCNNNNNNNAATTCTACACTAATTTCAGGATCCTGCAAAAACNATAGAAGAATGACCACTAAACTAACATCATNTTAGTTATTTCTACATTTGAGGAAGAAAGACNAATATTTATGATGGATTTTNAACGATCTGGTTCGGTCAGACTATGCCAAAGATGGGTATNTTAAGGACCATATAAAATAGGCCATATGACTTTTCAAAGTTTTGGCTTNACTGGTCTTATCATATGATCATGCATNCATACATATTGACNGTTGCCAAAATATTAGACAAAAATGAACATATNTGATCGTCCGATCTTACGAACGCATCTCCTTTATGGTCCAGATTNATAGATTCACTTTTCTTTTGTGNTCTTAAGNTCAAACTCGTCCAAACCAATAATAAACATGCATGCTTTTGATCTTCTAAACNTCCCTAAGTCGAGTTCATAATACACCATATCATGTGATCATGNGAAGCNAAATACTACCAAATGTGCATAAAATTAGCATAAAATGTCTCAAGGTTCAAANACTTTAATCTCATACAAAATCATCATCGTGATTTACAGAAACTAGATTTGGTCTTTGCAAAGACATTTTAATCTGAGCATCATCAATGTTACTANTACCATGTAAACGCTTATAAGTAGACAACTTCACTCTTTTTGTGGTCTCGTTGAGAGATAACGGCTGCAGCGGTTCCCGAGGTCTTTGTTGTGGTTTGTTAAAGGCACGCTTATTTACAGGTTGGTTCCGATTGTGCTCTTCGGACTCCACCGTTTTTGGACTGACATTTGATTCGATGGGAACGAGCTTCCCATCTACCTGGTTTGTGAAAACAAACGTCACCTGCAATGTTTTTAATAAACCATGAAAATAGTTATCATAAAGTTATTGGCTTTTCGAAAAATCTGACATAAGATGTAGAGTTTATGATTATNAGATTGAAAAAACTTGGAGACAACTCACCTCGTCACCTGCTGAAGCTTGCAGTATAGCATCTGGAACGGATTTTGAGGTAAAGTGATGACCCCAGCTTCCTAGATCTTCTTCCAATGGAGTGCCCACCTAAGAATATTCATGGGAAAAAAACTTTGTAGATGAATCGGATGTAAAAAGACATGAGCAGAGTTTGTAACTAACAATGGAATTGATGAAGAAATAGCTCAAAACAGACTTATATTTAATGCAGGACTCAGTAACAAGCACAAGAAGGGACCTGTCTCTCAGAGTNTTTTAACAAGTTAAAGCATCCTGCATCTTTTGCAAATCCGCCTATGTCTTCTTGGTCAATCTGGCAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.237531
PRIMER_LEFT_0_PENALTY=0.232299
PRIMER_RIGHT_0_PENALTY=0.005232
PRIMER_LEFT_0_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_0_SEQUENCE=CAGTCCAAAAACGGTGGAGT
PRIMER_LEFT_0=697,20
PRIMER_RIGHT_0=1671,20
PRIMER_LEFT_0_TM=60.232
PRIMER_RIGHT_0_TM=60.005
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=975
PRIMER_PAIR_1_PENALTY=0.294036
PRIMER_LEFT_1_PENALTY=0.288804
PRIMER_RIGHT_1_PENALTY=0.005232
PRIMER_LEFT_1_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_1_SEQUENCE=CAGTCCAAAAACGGTGGAGT
PRIMER_LEFT_1=832,20
PRIMER_RIGHT_1=1671,20
PRIMER_LEFT_1_TM=59.711
PRIMER_RIGHT_1_TM=60.005
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=840
PRIMER_PAIR_2_PENALTY=0.351076
PRIMER_LEFT_2_PENALTY=0.232299
PRIMER_RIGHT_2_PENALTY=0.118777
PRIMER_LEFT_2_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_2_SEQUENCE=AGAGCACAATCGGAACCAAC
PRIMER_LEFT_2=697,20
PRIMER_RIGHT_2=1647,20
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=60.119
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=951
PRIMER_PAIR_3_PENALTY=0.407581
PRIMER_LEFT_3_PENALTY=0.288804
PRIMER_RIGHT_3_PENALTY=0.118777
PRIMER_LEFT_3_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_3_SEQUENCE=AGAGCACAATCGGAACCAAC
PRIMER_LEFT_3=832,20
PRIMER_RIGHT_3=1647,20
PRIMER_LEFT_3_TM=59.711
PRIMER_RIGHT_3_TM=60.119
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=816
PRIMER_PAIR_4_PENALTY=0.422641
PRIMER_LEFT_4_PENALTY=0.232299
PRIMER_RIGHT_4_PENALTY=0.190341
PRIMER_LEFT_4_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_4_SEQUENCE=GGAGTCCGAAGAGCACAATC
PRIMER_LEFT_4=697,20
PRIMER_RIGHT_4=1656,20
PRIMER_LEFT_4_TM=60.232
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=960
PRIMER_PAIR_5_PENALTY=0.479145
PRIMER_LEFT_5_PENALTY=0.288804
PRIMER_RIGHT_5_PENALTY=0.190341
PRIMER_LEFT_5_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_5_SEQUENCE=GGAGTCCGAAGAGCACAATC
PRIMER_LEFT_5=832,20
PRIMER_RIGHT_5=1656,20
PRIMER_LEFT_5_TM=59.711
PRIMER_RIGHT_5_TM=59.810
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=825
PRIMER_PAIR_6_PENALTY=0.548960
PRIMER_LEFT_6_PENALTY=0.288804
PRIMER_RIGHT_6_PENALTY=0.260155
PRIMER_LEFT_6_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_6_SEQUENCE=GCAGGTGACGTTTGTTTTCA
PRIMER_LEFT_6=832,20
PRIMER_RIGHT_6=1734,20
PRIMER_LEFT_6_TM=59.711
PRIMER_RIGHT_6_TM=59.740
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=903
PRIMER_PAIR_7_PENALTY=0.548960
PRIMER_LEFT_7_PENALTY=0.288804
PRIMER_RIGHT_7_PENALTY=0.260155
PRIMER_LEFT_7_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_7_SEQUENCE=TGCAGGTGACGTTTGTTTTC
PRIMER_LEFT_7=832,20
PRIMER_RIGHT_7=1735,20
PRIMER_LEFT_7_TM=59.711
PRIMER_RIGHT_7_TM=59.740
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=904
PRIMER_PAIR_8_PENALTY=0.605657
PRIMER_LEFT_8_PENALTY=0.232299
PRIMER_RIGHT_8_PENALTY=0.373358
PRIMER_LEFT_8_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_8_SEQUENCE=CGTGCCTTTAACAAACCACA
PRIMER_LEFT_8=697,20
PRIMER_RIGHT_8=1616,20
PRIMER_LEFT_8_TM=60.232
PRIMER_RIGHT_8_TM=59.627
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.1000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=920
PRIMER_PAIR_9_PENALTY=0.662162
PRIMER_LEFT_9_PENALTY=0.288804
PRIMER_RIGHT_9_PENALTY=0.373358
PRIMER_LEFT_9_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_9_SEQUENCE=CGTGCCTTTAACAAACCACA
PRIMER_LEFT_9=832,20
PRIMER_RIGHT_9=1616,20
PRIMER_LEFT_9_TM=59.711
PRIMER_RIGHT_9_TM=59.627
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=785
PRIMER_PAIR_10_PENALTY=0.673633
PRIMER_LEFT_10_PENALTY=0.288804
PRIMER_RIGHT_10_PENALTY=0.384829
PRIMER_LEFT_10_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_10_SEQUENCE=CATTGCAGGTGACGTTTGTT
PRIMER_LEFT_10=832,20
PRIMER_RIGHT_10=1738,20
PRIMER_LEFT_10_TM=59.711
PRIMER_RIGHT_10_TM=59.615
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=7.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=907
PRIMER_PAIR_11_PENALTY=0.673633
PRIMER_LEFT_11_PENALTY=0.288804
PRIMER_RIGHT_11_PENALTY=0.384829
PRIMER_LEFT_11_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_11_SEQUENCE=AACATTGCAGGTGACGTTTG
PRIMER_LEFT_11=832,20
PRIMER_RIGHT_11=1740,20
PRIMER_LEFT_11_TM=59.711
PRIMER_RIGHT_11_TM=59.615
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=7.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=909
PRIMER_PAIR_12_PENALTY=0.900845
PRIMER_LEFT_12_PENALTY=0.232299
PRIMER_RIGHT_12_PENALTY=0.668546
PRIMER_LEFT_12_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_12_SEQUENCE=AGCGTGCCTTTAACAAACCA
PRIMER_LEFT_12=697,20
PRIMER_RIGHT_12=1618,20
PRIMER_LEFT_12_TM=60.232
PRIMER_RIGHT_12_TM=60.669
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.1000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=922
PRIMER_PAIR_13_PENALTY=0.957350
PRIMER_LEFT_13_PENALTY=0.288804
PRIMER_RIGHT_13_PENALTY=0.668546
PRIMER_LEFT_13_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_13_SEQUENCE=AGCGTGCCTTTAACAAACCA
PRIMER_LEFT_13=832,20
PRIMER_RIGHT_13=1618,20
PRIMER_LEFT_13_TM=59.711
PRIMER_RIGHT_13_TM=60.669
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=787
PRIMER_PAIR_14_PENALTY=0.961439
PRIMER_LEFT_14_PENALTY=0.232299
PRIMER_RIGHT_14_PENALTY=0.729140
PRIMER_LEFT_14_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_14_SEQUENCE=AAGCGTGCCTTTAACAAACC
PRIMER_LEFT_14=697,20
PRIMER_RIGHT_14=1619,20
PRIMER_LEFT_14_TM=60.232
PRIMER_RIGHT_14_TM=59.271
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=923
PRIMER_PAIR_15_PENALTY=0.980367
PRIMER_LEFT_15_PENALTY=0.288804
PRIMER_RIGHT_15_PENALTY=0.691563
PRIMER_LEFT_15_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_15_SEQUENCE=CAGGTAGATGGGAAGCTCGT
PRIMER_LEFT_15=832,20
PRIMER_RIGHT_15=1709,20
PRIMER_LEFT_15_TM=59.711
PRIMER_RIGHT_15_TM=59.308
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=878
PRIMER_PAIR_16_PENALTY=1.017944
PRIMER_LEFT_16_PENALTY=0.288804
PRIMER_RIGHT_16_PENALTY=0.729140
PRIMER_LEFT_16_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_16_SEQUENCE=AAGCGTGCCTTTAACAAACC
PRIMER_LEFT_16=832,20
PRIMER_RIGHT_16=1619,20
PRIMER_LEFT_16_TM=59.711
PRIMER_RIGHT_16_TM=59.271
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=788
PRIMER_PAIR_17_PENALTY=1.058572
PRIMER_LEFT_17_PENALTY=0.232299
PRIMER_RIGHT_17_PENALTY=0.826272
PRIMER_LEFT_17_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_17_SEQUENCE=CTGCAGCCGTTATCTCTCAA
PRIMER_LEFT_17=697,20
PRIMER_RIGHT_17=1577,20
PRIMER_LEFT_17_TM=60.232
PRIMER_RIGHT_17_TM=59.174
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.1000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=881
PRIMER_PAIR_18_PENALTY=1.115077
PRIMER_LEFT_18_PENALTY=0.288804
PRIMER_RIGHT_18_PENALTY=0.826272
PRIMER_LEFT_18_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_18_SEQUENCE=CTGCAGCCGTTATCTCTCAA
PRIMER_LEFT_18=832,20
PRIMER_RIGHT_18=1577,20
PRIMER_LEFT_18_TM=59.711
PRIMER_RIGHT_18_TM=59.174
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=746
PRIMER_PAIR_19_PENALTY=1.165903
PRIMER_LEFT_19_PENALTY=0.232299
PRIMER_RIGHT_19_PENALTY=0.933604
PRIMER_LEFT_19_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_19_SEQUENCE=GTCAGTCCAAAAACGGTGGA
PRIMER_LEFT_19=697,20
PRIMER_RIGHT_19=1673,20
PRIMER_LEFT_19_TM=60.232
PRIMER_RIGHT_19_TM=60.934
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.1000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=977
PRIMER_PAIR_20_PENALTY=1.192444
PRIMER_LEFT_20_PENALTY=0.232299
PRIMER_RIGHT_20_PENALTY=0.960145
PRIMER_LEFT_20_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_20_SEQUENCE=ACAACAAAGACCTCGGGAAC
PRIMER_LEFT_20=697,20
PRIMER_RIGHT_20=1599,20
PRIMER_LEFT_20_TM=60.232
PRIMER_RIGHT_20_TM=59.040
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.1000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=903
PRIMER_PAIR_21_PENALTY=1.203902
PRIMER_LEFT_21_PENALTY=0.232299
PRIMER_RIGHT_21_PENALTY=0.971603
PRIMER_LEFT_21_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_21_SEQUENCE=CTCGTTCCCATCGAATCAAA
PRIMER_LEFT_21=697,20
PRIMER_RIGHT_21=1694,20
PRIMER_LEFT_21_TM=60.232
PRIMER_RIGHT_21_TM=60.972
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.1000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=998
PRIMER_PAIR_22_PENALTY=1.204553
PRIMER_LEFT_22_PENALTY=0.232299
PRIMER_RIGHT_22_PENALTY=0.972254
PRIMER_LEFT_22_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_22_SEQUENCE=TGCAGCCGTTATCTCTCAAC
PRIMER_LEFT_22=697,20
PRIMER_RIGHT_22=1576,20
PRIMER_LEFT_22_TM=60.232
PRIMER_RIGHT_22_TM=59.028
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.1000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=880
PRIMER_PAIR_23_PENALTY=1.207916
PRIMER_LEFT_23_PENALTY=0.232299
PRIMER_RIGHT_23_PENALTY=0.975617
PRIMER_LEFT_23_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_23_SEQUENCE=CAGCCGTTATCTCTCAACGA
PRIMER_LEFT_23=697,20
PRIMER_RIGHT_23=1574,20
PRIMER_LEFT_23_TM=60.232
PRIMER_RIGHT_23_TM=59.024
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.1000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=878
PRIMER_PAIR_24_PENALTY=1.222408
PRIMER_LEFT_24_PENALTY=0.288804
PRIMER_RIGHT_24_PENALTY=0.933604
PRIMER_LEFT_24_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_24_SEQUENCE=GTCAGTCCAAAAACGGTGGA
PRIMER_LEFT_24=832,20
PRIMER_RIGHT_24=1673,20
PRIMER_LEFT_24_TM=59.711
PRIMER_RIGHT_24_TM=60.934
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=842
PRIMER_PAIR_25_PENALTY=1.225296
PRIMER_LEFT_25_PENALTY=0.288804
PRIMER_RIGHT_25_PENALTY=0.936492
PRIMER_LEFT_25_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_25_SEQUENCE=ACATTGCAGGTGACGTTTGT
PRIMER_LEFT_25=832,20
PRIMER_RIGHT_25=1739,20
PRIMER_LEFT_25_TM=59.711
PRIMER_RIGHT_25_TM=59.064
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=7.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=908
PRIMER_PAIR_26_PENALTY=1.248949
PRIMER_LEFT_26_PENALTY=0.288804
PRIMER_RIGHT_26_PENALTY=0.960145
PRIMER_LEFT_26_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_26_SEQUENCE=ACAACAAAGACCTCGGGAAC
PRIMER_LEFT_26=832,20
PRIMER_RIGHT_26=1599,20
PRIMER_LEFT_26_TM=59.711
PRIMER_RIGHT_26_TM=59.040
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=768
PRIMER_PAIR_27_PENALTY=1.260407
PRIMER_LEFT_27_PENALTY=0.288804
PRIMER_RIGHT_27_PENALTY=0.971603
PRIMER_LEFT_27_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_27_SEQUENCE=CTCGTTCCCATCGAATCAAA
PRIMER_LEFT_27=832,20
PRIMER_RIGHT_27=1694,20
PRIMER_LEFT_27_TM=59.711
PRIMER_RIGHT_27_TM=60.972
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=863
PRIMER_PAIR_28_PENALTY=1.261058
PRIMER_LEFT_28_PENALTY=0.288804
PRIMER_RIGHT_28_PENALTY=0.972254
PRIMER_LEFT_28_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_28_SEQUENCE=TGCAGCCGTTATCTCTCAAC
PRIMER_LEFT_28=832,20
PRIMER_RIGHT_28=1576,20
PRIMER_LEFT_28_TM=59.711
PRIMER_RIGHT_28_TM=59.028
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=745
PRIMER_PAIR_29_PENALTY=1.262144
PRIMER_LEFT_29_PENALTY=0.232299
PRIMER_RIGHT_29_PENALTY=1.029845
PRIMER_LEFT_29_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_29_SEQUENCE=CTGCAGCCGTTATCTCTCAAC
PRIMER_LEFT_29=697,20
PRIMER_RIGHT_29=1577,21
PRIMER_LEFT_29_TM=60.232
PRIMER_RIGHT_29_TM=60.030
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.1000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=881
PRIMER_PAIR_30_PENALTY=1.264421
PRIMER_LEFT_30_PENALTY=0.288804
PRIMER_RIGHT_30_PENALTY=0.975617
PRIMER_LEFT_30_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_30_SEQUENCE=CAGCCGTTATCTCTCAACGA
PRIMER_LEFT_30=832,20
PRIMER_RIGHT_30=1574,20
PRIMER_LEFT_30_TM=59.711
PRIMER_RIGHT_30_TM=59.024
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=2.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=743
PRIMER_PAIR_31_PENALTY=1.272725
PRIMER_LEFT_31_PENALTY=1.267494
PRIMER_RIGHT_31_PENALTY=0.005232
PRIMER_LEFT_31_SEQUENCE=TAAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_31_SEQUENCE=CAGTCCAAAAACGGTGGAGT
PRIMER_LEFT_31=831,21
PRIMER_RIGHT_31=1671,20
PRIMER_LEFT_31_TM=59.733
PRIMER_RIGHT_31_TM=60.005
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=7.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=841
PRIMER_PAIR_32_PENALTY=1.272725
PRIMER_LEFT_32_PENALTY=1.267494
PRIMER_RIGHT_32_PENALTY=0.005232
PRIMER_LEFT_32_SEQUENCE=TTAAGTCCTCCACCAGCAAAA
PRIMER_RIGHT_32_SEQUENCE=CAGTCCAAAAACGGTGGAGT
PRIMER_LEFT_32=830,21
PRIMER_RIGHT_32=1671,20
PRIMER_LEFT_32_TM=59.733
PRIMER_RIGHT_32_TM=60.005
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=842
PRIMER_PAIR_33_PENALTY=1.296125
PRIMER_LEFT_33_PENALTY=0.232299
PRIMER_RIGHT_33_PENALTY=1.063825
PRIMER_LEFT_33_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_33_SEQUENCE=CACAACAAAGACCTCGGGAA
PRIMER_LEFT_33=697,20
PRIMER_RIGHT_33=1600,20
PRIMER_LEFT_33_TM=60.232
PRIMER_RIGHT_33_TM=61.064
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.1000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=904
PRIMER_PAIR_34_PENALTY=1.296125
PRIMER_LEFT_34_PENALTY=0.232299
PRIMER_RIGHT_34_PENALTY=1.063825
PRIMER_LEFT_34_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_34_SEQUENCE=TCAGTCCAAAAACGGTGGAG
PRIMER_LEFT_34=697,20
PRIMER_RIGHT_34=1672,20
PRIMER_LEFT_34_TM=60.232
PRIMER_RIGHT_34_TM=61.064
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.1000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=976
PRIMER_PAIR_35_PENALTY=1.309019
PRIMER_LEFT_35_PENALTY=0.232299
PRIMER_RIGHT_35_PENALTY=1.076720
PRIMER_LEFT_35_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_35_SEQUENCE=CCCATCGAATCAAATGTCAG
PRIMER_LEFT_35=697,20
PRIMER_RIGHT_35=1688,20
PRIMER_LEFT_35_TM=60.232
PRIMER_RIGHT_35_TM=58.923
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.1000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=1.318649
PRIMER_LEFT_36_PENALTY=0.288804
PRIMER_RIGHT_36_PENALTY=1.029845
PRIMER_LEFT_36_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_36_SEQUENCE=CTGCAGCCGTTATCTCTCAAC
PRIMER_LEFT_36=832,20
PRIMER_RIGHT_36=1577,21
PRIMER_LEFT_36_TM=59.711
PRIMER_RIGHT_36_TM=60.030
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=746
PRIMER_PAIR_37_PENALTY=1.348943
PRIMER_LEFT_37_PENALTY=0.232299
PRIMER_RIGHT_37_PENALTY=1.116644
PRIMER_LEFT_37_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_37_SEQUENCE=AATGTCAGTCCAAAAACGGTG
PRIMER_LEFT_37=697,20
PRIMER_RIGHT_37=1676,21
PRIMER_LEFT_37_TM=60.232
PRIMER_RIGHT_37_TM=59.883
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.1000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=980
PRIMER_PAIR_38_PENALTY=1.352630
PRIMER_LEFT_38_PENALTY=0.288804
PRIMER_RIGHT_38_PENALTY=1.063825
PRIMER_LEFT_38_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_38_SEQUENCE=CACAACAAAGACCTCGGGAA
PRIMER_LEFT_38=832,20
PRIMER_RIGHT_38=1600,20
PRIMER_LEFT_38_TM=59.711
PRIMER_RIGHT_38_TM=61.064
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=769
PRIMER_PAIR_39_PENALTY=1.352630
PRIMER_LEFT_39_PENALTY=0.288804
PRIMER_RIGHT_39_PENALTY=1.063825
PRIMER_LEFT_39_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_39_SEQUENCE=TCAGTCCAAAAACGGTGGAG
PRIMER_LEFT_39=832,20
PRIMER_RIGHT_39=1672,20
PRIMER_LEFT_39_TM=59.711
PRIMER_RIGHT_39_TM=61.064
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=7.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=841
PRIMER_PAIR_40_PENALTY=1.365524
PRIMER_LEFT_40_PENALTY=0.288804
PRIMER_RIGHT_40_PENALTY=1.076720
PRIMER_LEFT_40_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_40_SEQUENCE=CCCATCGAATCAAATGTCAG
PRIMER_LEFT_40=832,20
PRIMER_RIGHT_40=1688,20
PRIMER_LEFT_40_TM=59.711
PRIMER_RIGHT_40_TM=58.923
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=2.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=857
PRIMER_PAIR_41_PENALTY=1.386270
PRIMER_LEFT_41_PENALTY=1.267494
PRIMER_RIGHT_41_PENALTY=0.118777
PRIMER_LEFT_41_SEQUENCE=TAAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_41_SEQUENCE=AGAGCACAATCGGAACCAAC
PRIMER_LEFT_41=831,21
PRIMER_RIGHT_41=1647,20
PRIMER_LEFT_41_TM=59.733
PRIMER_RIGHT_41_TM=60.119
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=817
PRIMER_PAIR_42_PENALTY=1.386270
PRIMER_LEFT_42_PENALTY=1.267494
PRIMER_RIGHT_42_PENALTY=0.118777
PRIMER_LEFT_42_SEQUENCE=TTAAGTCCTCCACCAGCAAAA
PRIMER_RIGHT_42_SEQUENCE=AGAGCACAATCGGAACCAAC
PRIMER_LEFT_42=830,21
PRIMER_RIGHT_42=1647,20
PRIMER_LEFT_42_TM=59.733
PRIMER_RIGHT_42_TM=60.119
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=818
PRIMER_PAIR_43_PENALTY=1.405448
PRIMER_LEFT_43_PENALTY=0.288804
PRIMER_RIGHT_43_PENALTY=1.116644
PRIMER_LEFT_43_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_43_SEQUENCE=AATGTCAGTCCAAAAACGGTG
PRIMER_LEFT_43=832,20
PRIMER_RIGHT_43=1676,21
PRIMER_LEFT_43_TM=59.711
PRIMER_RIGHT_43_TM=59.883
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=9.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=845
PRIMER_PAIR_44_PENALTY=1.420288
PRIMER_LEFT_44_PENALTY=0.232299
PRIMER_RIGHT_44_PENALTY=1.187989
PRIMER_LEFT_44_SEQUENCE=TTTGGCTGTCTTCTTGTCCC
PRIMER_RIGHT_44_SEQUENCE=GAAGAGCACAATCGGAACCA
PRIMER_LEFT_44=697,20
PRIMER_RIGHT_44=1649,20
PRIMER_LEFT_44_TM=60.232
PRIMER_RIGHT_44_TM=61.188
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.1000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=953
PRIMER_PAIR_45_PENALTY=1.423955
PRIMER_LEFT_45_PENALTY=1.305179
PRIMER_RIGHT_45_PENALTY=0.118777
PRIMER_LEFT_45_SEQUENCE=CAGAGGACTTTTGTGGTGGA
PRIMER_RIGHT_45_SEQUENCE=AGAGCACAATCGGAACCAAC
PRIMER_LEFT_45=652,20
PRIMER_RIGHT_45=1647,20
PRIMER_LEFT_45_TM=58.695
PRIMER_RIGHT_45_TM=60.119
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=996
PRIMER_PAIR_46_PENALTY=1.423955
PRIMER_LEFT_46_PENALTY=1.305179
PRIMER_RIGHT_46_PENALTY=0.118777
PRIMER_LEFT_46_SEQUENCE=TCAGAGGACTTTTGTGGTGG
PRIMER_RIGHT_46_SEQUENCE=AGAGCACAATCGGAACCAAC
PRIMER_LEFT_46=651,20
PRIMER_RIGHT_46=1647,20
PRIMER_LEFT_46_TM=58.695
PRIMER_RIGHT_46_TM=60.119
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=997
PRIMER_PAIR_47_PENALTY=1.450657
PRIMER_LEFT_47_PENALTY=0.288804
PRIMER_RIGHT_47_PENALTY=1.161853
PRIMER_LEFT_47_SEQUENCE=AAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_47_SEQUENCE=CACAAACCAGGTAGATGGGAA
PRIMER_LEFT_47=832,20
PRIMER_RIGHT_47=1716,21
PRIMER_LEFT_47_TM=59.711
PRIMER_RIGHT_47_TM=59.838
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=885
PRIMER_PAIR_48_PENALTY=1.457835
PRIMER_LEFT_48_PENALTY=1.267494
PRIMER_RIGHT_48_PENALTY=0.190341
PRIMER_LEFT_48_SEQUENCE=TAAGTCCTCCACCAGCAAAAA
PRIMER_RIGHT_48_SEQUENCE=GGAGTCCGAAGAGCACAATC
PRIMER_LEFT_48=831,21
PRIMER_RIGHT_48=1656,20
PRIMER_LEFT_48_TM=59.733
PRIMER_RIGHT_48_TM=59.810
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=826
PRIMER_PAIR_49_PENALTY=1.457835
PRIMER_LEFT_49_PENALTY=1.267494
PRIMER_RIGHT_49_PENALTY=0.190341
PRIMER_LEFT_49_SEQUENCE=TTAAGTCCTCCACCAGCAAAA
PRIMER_RIGHT_49_SEQUENCE=GGAGTCCGAAGAGCACAATC
PRIMER_LEFT_49=830,21
PRIMER_RIGHT_49=1656,20
PRIMER_LEFT_49_TM=59.733
PRIMER_RIGHT_49_TM=59.810
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=827
=
SEQUENCE_ID=MAGIC.287_3_22466007_22466444_bur-0_col-0_23628_28164_TRUE
SEQUENCE_TARGET=950,537
SEQUENCE_TEMPLATE=TTTTCCCAAGCCCTTTCTCTTATAAGGTTTCATTTTAATCCNTTTCTTTCTGAAGATTTTCTACANTTTAAATAGGTCTACCAATAAACTGATGTTTGGATAGATCATTTAGTACTTTTGAAGTTGTGGTAGAAGAAATGTTCTACACAACGTTTGGTGAAAGATATATATGTTANAGTTCATCAACTAAGAGATTTGTGAAATACNNNNNNNNNNNNNNNNAACGGTTTTTCGCGCCAAAAATCTAGCTAACTTATGATCTATCTTGTATTAGATAACGATTTTTCNCGGNATANTTTTCACATTATNNAAAATTANAATCNANAGATATGAAATTGATTANTGAATGNATTAGAATTGTATAGTGAATGAAGATCTATACAGAATTTACCTCTTGAGTTTACTCATCATAGAGAAAATATACCCACANATGTATCAATTGAATCTAATGANATACCTCTCTCNTTCAATTAACAGATTTAACTTATTGATGTTTGAAAGTGAATTAAACATATNAAAGTCATGCATAACTAGCTATACAACGCATCTCAACCTTTCCAAAGCGTTCAANATATATANNNAAACCAATGTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAAATATTATTGTATAACATATNACAGTTCAATTTGTGTTGTGNAGACTTTAAACAATCATCGTGGTCATCTAGAGAGCTGCACAATCAGCCATATTCAGTTCATTTTTTTGTTCATCGATAGGTGGATCCAAACAGTGACTCGTGAGAAACAAACCAAGGAGATTACTCTTGTCAACAATATAGGTTGCATGACACNTTTTGTTAGATATAATAGTCTTCACTTGTCCCCAAGTGCATTCTGTCATCNTAGCCTCACTTCACTCTCACTTACTCGATATAAGTTACTCGAGAAAGCCTTCAAGAACTGCTGCAATCTTAAGATCNTGAAGCTTTATGACATCATGTCCGATNTTAGTGTCCTTAGCAACGTTNTCAAAGCTTGCTCCTCTCTTGAGGTGCTTGTGTTGCAAATCACATTCTTAAACCAAGCAAGTGCGTTGAAGATCGAAAACAAGAAATTAGAATTCTTGCAAGTGACTTGGCCTTGTCTGATGAATAGGATGGAAGTGAATACACCTCGTTTGGTGATTTTTGACATCAAATCTATCTACTGTTTTGGCTACTCAGTTGAAGCCCCTAAATTAAGCATGTTTAAAAGAGATTATTGGGTTGGTGGNATGAGTTACCCTCATCTTAGCTACCATATATCAAGTCTCGCTCAGGTACAAGTACAAGCAACATGCATGCCTTTGGATATAATTTTGAACATAATATTTTGTTTTGACATGGTTTGTTGTATTATGAATTCAGGAGAAAATAAGAATTTGGCTTGAGCTTATGGTGAGCCANATTTATCATATGAAACGGANTGGATCCTTATCCGTGAGTGTCGATGTGAGAAATCCGAACGAAGTCGAGATTTTGAAGGAAGTCTTACTTTTGTGGGATGGAGAGATGATGGATCTGGAGATCCTCTTCAAGGTACATTTTACTTGATGNTTTTTTTTTTCTATGTGGATAAATAATTTGATCACTCACATGTCATATTTGCATTTTAGAACAACAATGCTCCTATAGAAGAAGGTGAATCTTTTATTACTGGTGGAGCACGTAACAAATGGTGGGATGGTGAAAAACCGTTTCCAGATGATTTCTTNCGCGTTTGTACTGTGTGGATGTATAATTTCGATGGTTCTAATGAAGAGGAATTTGCATTAGCGTCACGTTTTGTGACACAAGGAACAGTGACTGAAAAGTTGATGATAAAGACATCGACGTATCCTCCAGTGAAGCAATTAATGACAGAAGCAAAAGTGGCCAAGCTAATGGAACTTCCTAAAGGTTACGAGTATTTGGACATTGAATGCTTTTGATGATGGTTTTGGTTCGAATCTATCCATTGGTACTCTTTCCTTTATTACTCTGAGGATTATATGGTTTTAAATTTCAACAACTGTGTTTTTTTNGGTATTTGTTCTCTATTTTTAATGTGACATTTGGATATGTATTGGTTTGGATATTATTTGAGGTTTTTGTTTTGGTCAAATGGGATTATGTTATAATTGCATAACAAAACCATATTGGGTAAAAGCTGATTCAAACAAAAGAGTCAAGAGGATTTAGGGNAAATCATGTGTGTTCTACATNTATAATAAGATCATATCTATATATGAACTATTGAAGATGATGAACATATATGTGGCCAACAAAGCTAAATTGTAATCATCCTCTTTTCAAAACCCACAAATTTGATGATGACTTAGCCTTCCATGGGAAGACATCTACACTGCCTAAAGTGAAGCATCCATCTTCATGTTAGTAGATTTCTCCTTGACATGCCACTTGCTCTGTTTTTCTACGTATTGCTG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.548876
PRIMER_LEFT_0_PENALTY=0.519913
PRIMER_RIGHT_0_PENALTY=0.028963
PRIMER_LEFT_0_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_0_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_0=852,20
PRIMER_RIGHT_0=1703,20
PRIMER_LEFT_0_TM=60.520
PRIMER_RIGHT_0_TM=60.029
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=852
PRIMER_PAIR_1_PENALTY=0.548876
PRIMER_LEFT_1_PENALTY=0.519913
PRIMER_RIGHT_1_PENALTY=0.028963
PRIMER_LEFT_1_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_1_SEQUENCE=TTTTTCACCATCCCACCATT
PRIMER_LEFT_1=852,20
PRIMER_RIGHT_1=1704,20
PRIMER_LEFT_1_TM=60.520
PRIMER_RIGHT_1_TM=60.029
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=853
PRIMER_PAIR_2_PENALTY=0.762339
PRIMER_LEFT_2_PENALTY=0.519913
PRIMER_RIGHT_2_PENALTY=0.242426
PRIMER_LEFT_2_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_2_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_2=852,20
PRIMER_RIGHT_2=1686,20
PRIMER_LEFT_2_TM=60.520
PRIMER_RIGHT_2_TM=59.758
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=835
PRIMER_PAIR_3_PENALTY=0.808120
PRIMER_LEFT_3_PENALTY=0.519913
PRIMER_RIGHT_3_PENALTY=0.288208
PRIMER_LEFT_3_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_3_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_3=852,20
PRIMER_RIGHT_3=1529,20
PRIMER_LEFT_3_TM=60.520
PRIMER_RIGHT_3_TM=60.288
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=678
PRIMER_PAIR_4_PENALTY=0.924240
PRIMER_LEFT_4_PENALTY=0.636033
PRIMER_RIGHT_4_PENALTY=0.288208
PRIMER_LEFT_4_SEQUENCE=AACGCATCTCAACCTTTCCA
PRIMER_RIGHT_4_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_4=540,20
PRIMER_RIGHT_4=1529,20
PRIMER_LEFT_4_TM=60.636
PRIMER_RIGHT_4_TM=60.288
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=990
PRIMER_PAIR_5_PENALTY=0.924240
PRIMER_LEFT_5_PENALTY=0.636033
PRIMER_RIGHT_5_PENALTY=0.288208
PRIMER_LEFT_5_SEQUENCE=CAACGCATCTCAACCTTTCC
PRIMER_RIGHT_5_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_5=539,20
PRIMER_RIGHT_5=1529,20
PRIMER_LEFT_5_TM=60.636
PRIMER_RIGHT_5_TM=60.288
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=991
PRIMER_PAIR_6_PENALTY=0.939265
PRIMER_LEFT_6_PENALTY=0.910302
PRIMER_RIGHT_6_PENALTY=0.028963
PRIMER_LEFT_6_SEQUENCE=CCCAAGTGCATTCTGTCATC
PRIMER_RIGHT_6_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_6=855,20
PRIMER_RIGHT_6=1703,20
PRIMER_LEFT_6_TM=59.090
PRIMER_RIGHT_6_TM=60.029
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=849
PRIMER_PAIR_7_PENALTY=0.939265
PRIMER_LEFT_7_PENALTY=0.910302
PRIMER_RIGHT_7_PENALTY=0.028963
PRIMER_LEFT_7_SEQUENCE=CCCAAGTGCATTCTGTCATC
PRIMER_RIGHT_7_SEQUENCE=TTTTTCACCATCCCACCATT
PRIMER_LEFT_7=855,20
PRIMER_RIGHT_7=1704,20
PRIMER_LEFT_7_TM=59.090
PRIMER_RIGHT_7_TM=60.029
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=850
PRIMER_PAIR_8_PENALTY=0.952556
PRIMER_LEFT_8_PENALTY=0.710129
PRIMER_RIGHT_8_PENALTY=0.242426
PRIMER_LEFT_8_SEQUENCE=AGAGAGCTGCACAATCAGCC
PRIMER_RIGHT_8_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_8=699,20
PRIMER_RIGHT_8=1686,20
PRIMER_LEFT_8_TM=60.710
PRIMER_RIGHT_8_TM=59.758
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=988
PRIMER_PAIR_9_PENALTY=0.998337
PRIMER_LEFT_9_PENALTY=0.710129
PRIMER_RIGHT_9_PENALTY=0.288208
PRIMER_LEFT_9_SEQUENCE=AGAGAGCTGCACAATCAGCC
PRIMER_RIGHT_9_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_9=699,20
PRIMER_RIGHT_9=1529,20
PRIMER_LEFT_9_TM=60.710
PRIMER_RIGHT_9_TM=60.288
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=831
PRIMER_PAIR_10_PENALTY=1.094705
PRIMER_LEFT_10_PENALTY=0.519913
PRIMER_RIGHT_10_PENALTY=0.574792
PRIMER_LEFT_10_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_10_SEQUENCE=CATCATCTCTCCATCCCACA
PRIMER_LEFT_10=852,20
PRIMER_RIGHT_10=1528,20
PRIMER_LEFT_10_TM=60.520
PRIMER_RIGHT_10_TM=59.425
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=677
PRIMER_PAIR_11_PENALTY=1.147260
PRIMER_LEFT_11_PENALTY=0.519913
PRIMER_RIGHT_11_PENALTY=0.627348
PRIMER_LEFT_11_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_11_SEQUENCE=AAACGTGACGCTAATGCAAA
PRIMER_LEFT_11=852,20
PRIMER_RIGHT_11=1796,20
PRIMER_LEFT_11_TM=60.520
PRIMER_RIGHT_11_TM=59.373
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=945
PRIMER_PAIR_12_PENALTY=1.152728
PRIMER_LEFT_12_PENALTY=0.910302
PRIMER_RIGHT_12_PENALTY=0.242426
PRIMER_LEFT_12_SEQUENCE=CCCAAGTGCATTCTGTCATC
PRIMER_RIGHT_12_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_12=855,20
PRIMER_RIGHT_12=1686,20
PRIMER_LEFT_12_TM=59.090
PRIMER_RIGHT_12_TM=59.758
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=832
PRIMER_PAIR_13_PENALTY=1.198509
PRIMER_LEFT_13_PENALTY=0.910302
PRIMER_RIGHT_13_PENALTY=0.288208
PRIMER_LEFT_13_SEQUENCE=CCCAAGTGCATTCTGTCATC
PRIMER_RIGHT_13_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_13=855,20
PRIMER_RIGHT_13=1529,20
PRIMER_LEFT_13_TM=59.090
PRIMER_RIGHT_13_TM=60.288
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=675
PRIMER_PAIR_14_PENALTY=1.210825
PRIMER_LEFT_14_PENALTY=0.636033
PRIMER_RIGHT_14_PENALTY=0.574792
PRIMER_LEFT_14_SEQUENCE=AACGCATCTCAACCTTTCCA
PRIMER_RIGHT_14_SEQUENCE=CATCATCTCTCCATCCCACA
PRIMER_LEFT_14=540,20
PRIMER_RIGHT_14=1528,20
PRIMER_LEFT_14_TM=60.636
PRIMER_RIGHT_14_TM=59.425
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=989
PRIMER_PAIR_15_PENALTY=1.210825
PRIMER_LEFT_15_PENALTY=0.636033
PRIMER_RIGHT_15_PENALTY=0.574792
PRIMER_LEFT_15_SEQUENCE=CAACGCATCTCAACCTTTCC
PRIMER_RIGHT_15_SEQUENCE=CATCATCTCTCCATCCCACA
PRIMER_LEFT_15=539,20
PRIMER_RIGHT_15=1528,20
PRIMER_LEFT_15_TM=60.636
PRIMER_RIGHT_15_TM=59.425
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=990
PRIMER_PAIR_16_PENALTY=1.269357
PRIMER_LEFT_16_PENALTY=1.240394
PRIMER_RIGHT_16_PENALTY=0.028963
PRIMER_LEFT_16_SEQUENCE=CGTGAGAAACAAACCAAGGAG
PRIMER_RIGHT_16_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_16=769,21
PRIMER_RIGHT_16=1703,20
PRIMER_LEFT_16_TM=59.760
PRIMER_RIGHT_16_TM=60.029
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=935
PRIMER_PAIR_17_PENALTY=1.269357
PRIMER_LEFT_17_PENALTY=1.240394
PRIMER_RIGHT_17_PENALTY=0.028963
PRIMER_LEFT_17_SEQUENCE=CGTGAGAAACAAACCAAGGAG
PRIMER_RIGHT_17_SEQUENCE=TTTTTCACCATCCCACCATT
PRIMER_LEFT_17=769,21
PRIMER_RIGHT_17=1704,20
PRIMER_LEFT_17_TM=59.760
PRIMER_RIGHT_17_TM=60.029
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=936
PRIMER_PAIR_18_PENALTY=1.282675
PRIMER_LEFT_18_PENALTY=1.253711
PRIMER_RIGHT_18_PENALTY=0.028963
PRIMER_LEFT_18_SEQUENCE=CGTGAGAAACAAACCAAGGA
PRIMER_RIGHT_18_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_18=769,20
PRIMER_RIGHT_18=1703,20
PRIMER_LEFT_18_TM=58.746
PRIMER_RIGHT_18_TM=60.029
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=935
PRIMER_PAIR_19_PENALTY=1.282675
PRIMER_LEFT_19_PENALTY=1.253711
PRIMER_RIGHT_19_PENALTY=0.028963
PRIMER_LEFT_19_SEQUENCE=CGTGAGAAACAAACCAAGGA
PRIMER_RIGHT_19_SEQUENCE=TTTTTCACCATCCCACCATT
PRIMER_LEFT_19=769,20
PRIMER_RIGHT_19=1704,20
PRIMER_LEFT_19_TM=58.746
PRIMER_RIGHT_19_TM=60.029
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=936
PRIMER_PAIR_20_PENALTY=1.284921
PRIMER_LEFT_20_PENALTY=0.710129
PRIMER_RIGHT_20_PENALTY=0.574792
PRIMER_LEFT_20_SEQUENCE=AGAGAGCTGCACAATCAGCC
PRIMER_RIGHT_20_SEQUENCE=CATCATCTCTCCATCCCACA
PRIMER_LEFT_20=699,20
PRIMER_RIGHT_20=1528,20
PRIMER_LEFT_20_TM=60.710
PRIMER_RIGHT_20_TM=59.425
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=830
PRIMER_PAIR_21_PENALTY=1.326055
PRIMER_LEFT_21_PENALTY=1.297092
PRIMER_RIGHT_21_PENALTY=0.028963
PRIMER_LEFT_21_SEQUENCE=TGCACAATCAGCCATATTCAG
PRIMER_RIGHT_21_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_21=706,21
PRIMER_RIGHT_21=1703,20
PRIMER_LEFT_21_TM=59.703
PRIMER_RIGHT_21_TM=60.029
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=7.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=998
PRIMER_PAIR_22_PENALTY=1.326055
PRIMER_LEFT_22_PENALTY=1.297092
PRIMER_RIGHT_22_PENALTY=0.028963
PRIMER_LEFT_22_SEQUENCE=TGCACAATCAGCCATATTCAG
PRIMER_RIGHT_22_SEQUENCE=TTTTTCACCATCCCACCATT
PRIMER_LEFT_22=706,21
PRIMER_RIGHT_22=1704,20
PRIMER_LEFT_22_TM=59.703
PRIMER_RIGHT_22_TM=60.029
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=999
PRIMER_PAIR_23_PENALTY=1.326055
PRIMER_LEFT_23_PENALTY=1.297092
PRIMER_RIGHT_23_PENALTY=0.028963
PRIMER_LEFT_23_SEQUENCE=CTGCACAATCAGCCATATTCA
PRIMER_RIGHT_23_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_23=705,21
PRIMER_RIGHT_23=1703,20
PRIMER_LEFT_23_TM=59.703
PRIMER_RIGHT_23_TM=60.029
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=7.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=999
PRIMER_PAIR_24_PENALTY=1.326055
PRIMER_LEFT_24_PENALTY=1.297092
PRIMER_RIGHT_24_PENALTY=0.028963
PRIMER_LEFT_24_SEQUENCE=CTGCACAATCAGCCATATTCA
PRIMER_RIGHT_24_SEQUENCE=TTTTTCACCATCCCACCATT
PRIMER_LEFT_24=705,21
PRIMER_RIGHT_24=1704,20
PRIMER_LEFT_24_TM=59.703
PRIMER_RIGHT_24_TM=60.029
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=1000
PRIMER_PAIR_25_PENALTY=1.326529
PRIMER_LEFT_25_PENALTY=0.519913
PRIMER_RIGHT_25_PENALTY=0.806616
PRIMER_LEFT_25_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_25_SEQUENCE=CCCACCATTTGTTACGTGCT
PRIMER_LEFT_25=852,20
PRIMER_RIGHT_25=1693,20
PRIMER_LEFT_25_TM=60.520
PRIMER_RIGHT_25_TM=60.807
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=842
PRIMER_PAIR_26_PENALTY=1.378711
PRIMER_LEFT_26_PENALTY=1.349748
PRIMER_RIGHT_26_PENALTY=0.028963
PRIMER_LEFT_26_SEQUENCE=TGCACAATCAGCCATATTCA
PRIMER_RIGHT_26_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_26=706,20
PRIMER_RIGHT_26=1703,20
PRIMER_LEFT_26_TM=58.650
PRIMER_RIGHT_26_TM=60.029
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.9000
PRIMER_RIGHT_26_END_STABILITY=7.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=998
PRIMER_PAIR_27_PENALTY=1.378711
PRIMER_LEFT_27_PENALTY=1.349748
PRIMER_RIGHT_27_PENALTY=0.028963
PRIMER_LEFT_27_SEQUENCE=TGCACAATCAGCCATATTCA
PRIMER_RIGHT_27_SEQUENCE=TTTTTCACCATCCCACCATT
PRIMER_LEFT_27=706,20
PRIMER_RIGHT_27=1704,20
PRIMER_LEFT_27_TM=58.650
PRIMER_RIGHT_27_TM=60.029
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=8.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=999
PRIMER_PAIR_28_PENALTY=1.469849
PRIMER_LEFT_28_PENALTY=0.519913
PRIMER_RIGHT_28_PENALTY=0.949937
PRIMER_LEFT_28_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_28_SEQUENCE=CATTTGTTACGTGCTCCACC
PRIMER_LEFT_28=852,20
PRIMER_RIGHT_28=1688,20
PRIMER_LEFT_28_TM=60.520
PRIMER_RIGHT_28_TM=59.050
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=837
PRIMER_PAIR_29_PENALTY=1.469849
PRIMER_LEFT_29_PENALTY=0.519913
PRIMER_RIGHT_29_PENALTY=0.949937
PRIMER_LEFT_29_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_29_SEQUENCE=ACCATTTGTTACGTGCTCCA
PRIMER_LEFT_29=852,20
PRIMER_RIGHT_29=1690,20
PRIMER_LEFT_29_TM=60.520
PRIMER_RIGHT_29_TM=59.050
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=839
PRIMER_PAIR_30_PENALTY=1.469849
PRIMER_LEFT_30_PENALTY=0.519913
PRIMER_RIGHT_30_PENALTY=0.949937
PRIMER_LEFT_30_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_30_SEQUENCE=CACCATTTGTTACGTGCTCC
PRIMER_LEFT_30=852,20
PRIMER_RIGHT_30=1691,20
PRIMER_LEFT_30_TM=60.520
PRIMER_RIGHT_30_TM=59.050
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=840
PRIMER_PAIR_31_PENALTY=1.482821
PRIMER_LEFT_31_PENALTY=1.240394
PRIMER_RIGHT_31_PENALTY=0.242426
PRIMER_LEFT_31_SEQUENCE=CGTGAGAAACAAACCAAGGAG
PRIMER_RIGHT_31_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_31=769,21
PRIMER_RIGHT_31=1686,20
PRIMER_LEFT_31_TM=59.760
PRIMER_RIGHT_31_TM=59.758
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=918
PRIMER_PAIR_32_PENALTY=1.485094
PRIMER_LEFT_32_PENALTY=0.910302
PRIMER_RIGHT_32_PENALTY=0.574792
PRIMER_LEFT_32_SEQUENCE=CCCAAGTGCATTCTGTCATC
PRIMER_RIGHT_32_SEQUENCE=CATCATCTCTCCATCCCACA
PRIMER_LEFT_32=855,20
PRIMER_RIGHT_32=1528,20
PRIMER_LEFT_32_TM=59.090
PRIMER_RIGHT_32_TM=59.425
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=674
PRIMER_PAIR_33_PENALTY=1.496138
PRIMER_LEFT_33_PENALTY=1.253711
PRIMER_RIGHT_33_PENALTY=0.242426
PRIMER_LEFT_33_SEQUENCE=CGTGAGAAACAAACCAAGGA
PRIMER_RIGHT_33_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_33=769,20
PRIMER_RIGHT_33=1686,20
PRIMER_LEFT_33_TM=58.746
PRIMER_RIGHT_33_TM=59.758
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=918
PRIMER_PAIR_34_PENALTY=1.510948
PRIMER_LEFT_34_PENALTY=0.519913
PRIMER_RIGHT_34_PENALTY=0.991035
PRIMER_LEFT_34_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_34_SEQUENCE=TCATCTCTCCATCCCACAAA
PRIMER_LEFT_34=852,20
PRIMER_RIGHT_34=1526,20
PRIMER_LEFT_34_TM=60.520
PRIMER_RIGHT_34_TM=59.009
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=675
PRIMER_PAIR_35_PENALTY=1.516745
PRIMER_LEFT_35_PENALTY=0.710129
PRIMER_RIGHT_35_PENALTY=0.806616
PRIMER_LEFT_35_SEQUENCE=AGAGAGCTGCACAATCAGCC
PRIMER_RIGHT_35_SEQUENCE=CCCACCATTTGTTACGTGCT
PRIMER_LEFT_35=699,20
PRIMER_RIGHT_35=1693,20
PRIMER_LEFT_35_TM=60.710
PRIMER_RIGHT_35_TM=60.807
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=995
PRIMER_PAIR_36_PENALTY=1.528602
PRIMER_LEFT_36_PENALTY=1.240394
PRIMER_RIGHT_36_PENALTY=0.288208
PRIMER_LEFT_36_SEQUENCE=CGTGAGAAACAAACCAAGGAG
PRIMER_RIGHT_36_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_36=769,21
PRIMER_RIGHT_36=1529,20
PRIMER_LEFT_36_TM=59.760
PRIMER_RIGHT_36_TM=60.288
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=761
PRIMER_PAIR_37_PENALTY=1.537649
PRIMER_LEFT_37_PENALTY=0.910302
PRIMER_RIGHT_37_PENALTY=0.627348
PRIMER_LEFT_37_SEQUENCE=CCCAAGTGCATTCTGTCATC
PRIMER_RIGHT_37_SEQUENCE=AAACGTGACGCTAATGCAAA
PRIMER_LEFT_37=855,20
PRIMER_RIGHT_37=1796,20
PRIMER_LEFT_37_TM=59.090
PRIMER_RIGHT_37_TM=59.373
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=8.8000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=942
PRIMER_PAIR_38_PENALTY=1.539519
PRIMER_LEFT_38_PENALTY=1.297092
PRIMER_RIGHT_38_PENALTY=0.242426
PRIMER_LEFT_38_SEQUENCE=TGCACAATCAGCCATATTCAG
PRIMER_RIGHT_38_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_38=706,21
PRIMER_RIGHT_38=1686,20
PRIMER_LEFT_38_TM=59.703
PRIMER_RIGHT_38_TM=59.758
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=981
PRIMER_PAIR_39_PENALTY=1.539519
PRIMER_LEFT_39_PENALTY=1.297092
PRIMER_RIGHT_39_PENALTY=0.242426
PRIMER_LEFT_39_SEQUENCE=CTGCACAATCAGCCATATTCA
PRIMER_RIGHT_39_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_39=705,21
PRIMER_RIGHT_39=1686,20
PRIMER_LEFT_39_TM=59.703
PRIMER_RIGHT_39_TM=59.758
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=982
PRIMER_PAIR_40_PENALTY=1.541919
PRIMER_LEFT_40_PENALTY=1.253711
PRIMER_RIGHT_40_PENALTY=0.288208
PRIMER_LEFT_40_SEQUENCE=CGTGAGAAACAAACCAAGGA
PRIMER_RIGHT_40_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_40=769,20
PRIMER_RIGHT_40=1529,20
PRIMER_LEFT_40_TM=58.746
PRIMER_RIGHT_40_TM=60.288
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=9.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=761
PRIMER_PAIR_41_PENALTY=1.564699
PRIMER_LEFT_41_PENALTY=0.519913
PRIMER_RIGHT_41_PENALTY=1.044787
PRIMER_LEFT_41_SEQUENCE=GTCCCCAAGTGCATTCTGTC
PRIMER_RIGHT_41_SEQUENCE=ATTTGTTACGTGCTCCACCAG
PRIMER_LEFT_41=852,20
PRIMER_RIGHT_41=1687,21
PRIMER_LEFT_41_TM=60.520
PRIMER_RIGHT_41_TM=60.045
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=836
PRIMER_PAIR_42_PENALTY=1.585300
PRIMER_LEFT_42_PENALTY=1.297092
PRIMER_RIGHT_42_PENALTY=0.288208
PRIMER_LEFT_42_SEQUENCE=TGCACAATCAGCCATATTCAG
PRIMER_RIGHT_42_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_42=706,21
PRIMER_RIGHT_42=1529,20
PRIMER_LEFT_42_TM=59.703
PRIMER_RIGHT_42_TM=60.288
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=2.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=824
PRIMER_PAIR_43_PENALTY=1.585300
PRIMER_LEFT_43_PENALTY=1.297092
PRIMER_RIGHT_43_PENALTY=0.288208
PRIMER_LEFT_43_SEQUENCE=CTGCACAATCAGCCATATTCA
PRIMER_RIGHT_43_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_43=705,21
PRIMER_RIGHT_43=1529,20
PRIMER_LEFT_43_TM=59.703
PRIMER_RIGHT_43_TM=60.288
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=825
PRIMER_PAIR_44_PENALTY=1.592175
PRIMER_LEFT_44_PENALTY=1.349748
PRIMER_RIGHT_44_PENALTY=0.242426
PRIMER_LEFT_44_SEQUENCE=TGCACAATCAGCCATATTCA
PRIMER_RIGHT_44_SEQUENCE=TTTGTTACGTGCTCCACCAG
PRIMER_LEFT_44=706,20
PRIMER_RIGHT_44=1686,20
PRIMER_LEFT_44_TM=58.650
PRIMER_RIGHT_44_TM=59.758
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=981
PRIMER_PAIR_45_PENALTY=1.627068
PRIMER_LEFT_45_PENALTY=0.636033
PRIMER_RIGHT_45_PENALTY=0.991035
PRIMER_LEFT_45_SEQUENCE=AACGCATCTCAACCTTTCCA
PRIMER_RIGHT_45_SEQUENCE=TCATCTCTCCATCCCACAAA
PRIMER_LEFT_45=540,20
PRIMER_RIGHT_45=1526,20
PRIMER_LEFT_45_TM=60.636
PRIMER_RIGHT_45_TM=59.009
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=987
PRIMER_PAIR_46_PENALTY=1.627068
PRIMER_LEFT_46_PENALTY=0.636033
PRIMER_RIGHT_46_PENALTY=0.991035
PRIMER_LEFT_46_SEQUENCE=CAACGCATCTCAACCTTTCC
PRIMER_RIGHT_46_SEQUENCE=TCATCTCTCCATCCCACAAA
PRIMER_LEFT_46=539,20
PRIMER_RIGHT_46=1526,20
PRIMER_LEFT_46_TM=60.636
PRIMER_RIGHT_46_TM=59.009
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=988
PRIMER_PAIR_47_PENALTY=1.637956
PRIMER_LEFT_47_PENALTY=1.349748
PRIMER_RIGHT_47_PENALTY=0.288208
PRIMER_LEFT_47_SEQUENCE=TGCACAATCAGCCATATTCA
PRIMER_RIGHT_47_SEQUENCE=CCATCATCTCTCCATCCCAC
PRIMER_LEFT_47=706,20
PRIMER_RIGHT_47=1529,20
PRIMER_LEFT_47_TM=58.650
PRIMER_RIGHT_47_TM=60.288
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=2.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=824
PRIMER_PAIR_48_PENALTY=1.659097
PRIMER_LEFT_48_PENALTY=1.630133
PRIMER_RIGHT_48_PENALTY=0.028963
PRIMER_LEFT_48_SEQUENCE=GCTGCACAATCAGCCATATTC
PRIMER_RIGHT_48_SEQUENCE=TTTTCACCATCCCACCATTT
PRIMER_LEFT_48=704,21
PRIMER_RIGHT_48=1703,20
PRIMER_LEFT_48_TM=60.630
PRIMER_RIGHT_48_TM=60.029
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.9000
PRIMER_RIGHT_48_END_STABILITY=7.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=1000
PRIMER_PAIR_49_PENALTY=1.660066
PRIMER_LEFT_49_PENALTY=0.710129
PRIMER_RIGHT_49_PENALTY=0.949937
PRIMER_LEFT_49_SEQUENCE=AGAGAGCTGCACAATCAGCC
PRIMER_RIGHT_49_SEQUENCE=CATTTGTTACGTGCTCCACC
PRIMER_LEFT_49=699,20
PRIMER_RIGHT_49=1688,20
PRIMER_LEFT_49_TM=60.710
PRIMER_RIGHT_49_TM=59.050
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=990
=
SEQUENCE_ID=MAGIC.433_5_10075000_10075762_sf-2_zu-0_119380_1136_TRUE
SEQUENCE_TARGET=950,862
SEQUENCE_TEMPLATE=TTGTGNAAANAANAAACGACANAGAGNCNCATTTGCACTCGTTTGGTTTNACATCACNATCNCNTNTGTTTGTTNCTCTTTTNTTTTGTATCACTTTTGTTTGTTANCNNTTTGGNTCCGAATGGAGAAAGCNTATNNGGCNGAGCNGATCAAGCGNNNNNNNNNGAACAAAAGCGGATTAANCNGATNAAGCNGATNTAGNAGTATAACTGTGGATCANACGNANTTAGCNGTCNAAATAAAGATTTGAATGGTNAAAGCGGATNAATNTTGNAGATNATATTATATAATATTTAATTATNAATTAAATGATAAATAAANTAANNANANNTATTTAATAATTATTAAAACATNAAAAAANATATTTNAAATACAACATCNAAAATAGATTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCATAACATCACCATCAACTGTNTNTAAAGCTTCNNTATCCCTACATTGGTTTCAGAATTTGTGTNNATAACCNCNNTCANNNNNNNNNNNNNNNNNNNNNCATCATNTNAAAANTNTNAAATNCTTATAAAATTATGTANTCCCATTNTAGCCATNANAACTNTNTTCTGCACATNNGTATTATATCTANGAANTTCACAANGAATCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAATTATNCACTCNAANNTTTTGTCCATATAGNTACAGTTTTAACGACCANNTANNNNNNTAACAACTTTTTNCTANTTATAAATTACAAATTAGTAACNGAAAANGATTTANTAAAANATNTANCTANTTTGTTACAANNCGTAGCAANTTANCNACATTTTGCGAGACNNNNTNTTTTGTAACAAATATGTTACCAAATGNNTTTNAAATTTAGTTGCAAAGTTCCANAAACCAAAATTCGTCGCCGATTTTGTAACANCAGTTGAATAGAATATNNNTTAANGTTTTACGANTATTATNAACTANAAGATTTNTANNNATTAGCGACGATTTANNCATTTTNTTTTAGTNACAAAGTTGATANGAATTTAGTATCNNAANNTTTGTTGTAAATTTTCACAAAANANGTTTCGTNACAAACTTTTGTNACAAANTAANATTATTAATATTNTTTAATTTTTNCNACANTTGGGTCGGTAGCAANTAGAAANAAANTGNAGACTCACAATATGTTTCNTAGCAATTATGANANCNTTGTNACTGCCCCTGTCTCATANNAAAGGNCACTNAGTGTNCGNTAGCAAATTNGTNACCAAAGNCACTNNTNNTGCGACNCTATAATTNNAGCAAGTTGATTACAAAGCCACTTCTTTTGCNANANTCTTATTNTNGNAAATTTATGATGGTGTCTTNTCGATCTTCCACCAANTTNTTGTTTANTTATTGGCTTCGTTNTCTCTNTTGTTCGNANNTATATATATGCNTCNTTNTTCTTTGTTGTATATTCATTCAAATNTTTGGCTTCTCGTTTTCTCTCTTNATATCTAGCAANAAAAAAAANTTCTTTCATATTCATTCTAAAATTTTTCATTTGAGNAAACCNTCTCTTTTTTCNTNTNANNNTATTCTATTATCTTNTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_22494608_22494627_col-0_ct-1_28164_45266_TRUE
SEQUENCE_TARGET=950,119
SEQUENCE_TEMPLATE=CGTCAGTTTACACGGACGGTTTGAGATTCTTTCTCTCTCGGGTTCGTTTCTTCCTCCACCGGCACCACCAGCTGCGTCTGGTTTAAAGGTTTACTTAGCCGGTGGTCAAGGTCAAGTGATCGGAGGCAGTGTGGTGGGACCGCTTACGGCATCAAGTCCGGTGGTCGTTATGGCAGCTTCATTTGGAAACGCATCTTACGAGAGGCTGCCACTAGAGGAGNAGGAGGAAACTGAAAGANAAATAGATGGAAACGCGGCTAGGGCGATTGGAACGCAAACGCAGAAACAGTTAATGCAAGATGCNACATCGTTTATTGGGTCGCCGTCGAATTTAATTAACTCTGTTTCGTTGCCAGGTGAAGCTTATTGGGGAACGCAACGACCGTCTTTCTAAGATAATATCATTGATAATATAAGTTTCGTCTTCTTATTCTTTTTCACTTTTTACCTTTTTCACTTTCTTAGGTTTTGTTTTAACGTTTGATTAATACCTNAAGGTTTTTGGAAAATTTTCGATCGGATAAAAGGATTTATGTTGCGAGCCGAAACGCAACGGTCTGATCTCGGATTGATCTTTATATATATAGGGGCTAGTGTTGTGATCTTCCTTGAGTTGATGTTTATAATGTAGTAGTTGATATTGAATGAAATCTTCATGTTTTTTGATAAATGATTATAGCTTGATCAAGATTTAGAAGTTAGCATAGAAAAATGCATGAGAAGACTGGTTAAAATTTTCGTGCACAAGTAGCTAAAGAGAATAATCTTGCTTCCTTGTTTAATGGCAAGACTTGGGAACAAGATGATTACATTGGCATACACTTTTCTAGACTTTTTTCTAGGTTATAGACACAAGTCAATACTTTGTATTCTAGTTACATTTATAAAACTAAAATTTCGATNGTAAACTATAGAACAAAAAATTATGAAAATCAATAATTGTACCCTGGTTAACTTATAACTAAGANCATCCCCAACGATATATCTTAGCCTAATCTTANCAAACATNTTAGCATNAGNTTAAGAAGTANTTCGTTCTTAACNATTGNNGNNNNNNTAANANCTTAAAAAAATTNTCCTAAANNNNNNNNNNNNNNNNNNNNNNNNCAGAAAAAGTCTAGCCCGGAAATCTCTGGACGTGTGGGCCGAATCTAAACCCTCTTAAAGCCCNATTTGATTAGGATCAAGAGAAAGGGGAAGCCCTAAAGATTGGCNTCCTGCGTTCATCATCACCNTCTCTCCCGCNGCTTTCTTTCATCTTTACAAAACCGATTACTTTTGTCGGAATCTGACGTCAGCATACCTGCTACCACTTCCAGAGTTCAATCGATCTNNNNNTCGTTGAAACGTTTTCCCTCTTCGATTATCAACAGTGTCTCCACTCGTATCATCACCGCCTCCAACTAATTTTGCCATCTCTCTCGTTGTTCCAATTTGATTCTACTAGTGAGCTCCGATTTAATTTAGGGTTTTGAAAGATGTCAGNTTTGAAAAATTACGGCTTGATCTCCATTGATTCTGCCCTCCATTTTCCTCGATCAAATCAATTGCAGAGCTATAAGAGGTTNNNNNNNNNNNNNNNNNNNCTCTCTNTGCCTAATCTTATTGGGATTATAGTTACTGGTATTGGATTTGGTTTCGACAGCTCTCTAGTGTAATTGAAATTTTTGTATCACAGGTAGAACANTGAATATATTGNNTTGGTTACTAAAGCAATAACTTTTTCTCTTGAAGAATCGGATTTGTAGTTGTGTTTATATCTGTTGATCGATTGGCTAGATTAATGCTTAGACATTGCCTTATGATCTCTGAGTGATTGATTTATTTGCTCATCAAACCAGAAGGAATGNAAAATGGGTCTCTCCAATAGCAGCTGTTGTACCTAACTTCCATCTTCCTATGCGNAGTCTCGAGGATAAAAACAGGTGCATCTTCTATTCCTTCTCATGTTTACTATTGTTTTTGTTCATACTTCAGCAGCTNTCTCTTGTATTTTTGTGTTGGCAGGACAAACACAGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.405748
PRIMER_LEFT_0_PENALTY=0.066323
PRIMER_RIGHT_0_PENALTY=0.339425
PRIMER_LEFT_0_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_0_SEQUENCE=TTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_0=353,20
PRIMER_RIGHT_0=1206,20
PRIMER_LEFT_0_TM=60.066
PRIMER_RIGHT_0_TM=59.661
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=854
PRIMER_PAIR_1_PENALTY=0.405748
PRIMER_LEFT_1_PENALTY=0.066323
PRIMER_RIGHT_1_PENALTY=0.339425
PRIMER_LEFT_1_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_1_SEQUENCE=TCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_1=353,20
PRIMER_RIGHT_1=1208,20
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=59.661
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=0.570697
PRIMER_LEFT_2_PENALTY=0.231272
PRIMER_RIGHT_2_PENALTY=0.339425
PRIMER_LEFT_2_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_2_SEQUENCE=TTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_2=338,20
PRIMER_RIGHT_2=1206,20
PRIMER_LEFT_2_TM=59.769
PRIMER_RIGHT_2_TM=59.661
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=869
PRIMER_PAIR_3_PENALTY=0.570697
PRIMER_LEFT_3_PENALTY=0.231272
PRIMER_RIGHT_3_PENALTY=0.339425
PRIMER_LEFT_3_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_3_SEQUENCE=TCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_3=338,20
PRIMER_RIGHT_3=1208,20
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=59.661
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=871
PRIMER_PAIR_4_PENALTY=0.618606
PRIMER_LEFT_4_PENALTY=0.066323
PRIMER_RIGHT_4_PENALTY=0.552282
PRIMER_LEFT_4_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_4_SEQUENCE=TCCGGGCTAGACTTTTTCTG
PRIMER_LEFT_4=353,20
PRIMER_RIGHT_4=1126,20
PRIMER_LEFT_4_TM=60.066
PRIMER_RIGHT_4_TM=59.448
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=774
PRIMER_PAIR_5_PENALTY=0.763236
PRIMER_LEFT_5_PENALTY=0.066323
PRIMER_RIGHT_5_PENALTY=0.696913
PRIMER_LEFT_5_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_5_SEQUENCE=TAGGGCTTCCCCTTTCTCTT
PRIMER_LEFT_5=353,20
PRIMER_RIGHT_5=1204,20
PRIMER_LEFT_5_TM=60.066
PRIMER_RIGHT_5_TM=59.303
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=10.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=852
PRIMER_PAIR_6_PENALTY=0.763236
PRIMER_LEFT_6_PENALTY=0.066323
PRIMER_RIGHT_6_PENALTY=0.696913
PRIMER_LEFT_6_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_6_SEQUENCE=TTAGGGCTTCCCCTTTCTCT
PRIMER_LEFT_6=353,20
PRIMER_RIGHT_6=1205,20
PRIMER_LEFT_6_TM=60.066
PRIMER_RIGHT_6_TM=59.303
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.9000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=853
PRIMER_PAIR_7_PENALTY=0.783555
PRIMER_LEFT_7_PENALTY=0.231272
PRIMER_RIGHT_7_PENALTY=0.552282
PRIMER_LEFT_7_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_7_SEQUENCE=TCCGGGCTAGACTTTTTCTG
PRIMER_LEFT_7=338,20
PRIMER_RIGHT_7=1126,20
PRIMER_LEFT_7_TM=59.769
PRIMER_RIGHT_7_TM=59.448
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=789
PRIMER_PAIR_8_PENALTY=0.859171
PRIMER_LEFT_8_PENALTY=0.814339
PRIMER_RIGHT_8_PENALTY=0.044832
PRIMER_LEFT_8_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_8_SEQUENCE=ATTAGTTGGAGGCGGTGATG
PRIMER_LEFT_8=524,20
PRIMER_RIGHT_8=1407,20
PRIMER_LEFT_8_TM=59.186
PRIMER_RIGHT_8_TM=59.955
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=884
PRIMER_PAIR_9_PENALTY=0.928185
PRIMER_LEFT_9_PENALTY=0.231272
PRIMER_RIGHT_9_PENALTY=0.696913
PRIMER_LEFT_9_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_9_SEQUENCE=TAGGGCTTCCCCTTTCTCTT
PRIMER_LEFT_9=338,20
PRIMER_RIGHT_9=1204,20
PRIMER_LEFT_9_TM=59.769
PRIMER_RIGHT_9_TM=59.303
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=867
PRIMER_PAIR_10_PENALTY=0.928185
PRIMER_LEFT_10_PENALTY=0.231272
PRIMER_RIGHT_10_PENALTY=0.696913
PRIMER_LEFT_10_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_10_SEQUENCE=TTAGGGCTTCCCCTTTCTCT
PRIMER_LEFT_10=338,20
PRIMER_RIGHT_10=1205,20
PRIMER_LEFT_10_TM=59.769
PRIMER_RIGHT_10_TM=59.303
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=868
PRIMER_PAIR_11_PENALTY=1.141767
PRIMER_LEFT_11_PENALTY=0.066323
PRIMER_RIGHT_11_PENALTY=1.075444
PRIMER_LEFT_11_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_11_SEQUENCE=ATCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_11=353,20
PRIMER_RIGHT_11=1209,21
PRIMER_LEFT_11_TM=60.066
PRIMER_RIGHT_11_TM=59.925
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.9000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=7.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=857
PRIMER_PAIR_12_PENALTY=1.153764
PRIMER_LEFT_12_PENALTY=0.814339
PRIMER_RIGHT_12_PENALTY=0.339425
PRIMER_LEFT_12_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_12_SEQUENCE=TTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_12=524,20
PRIMER_RIGHT_12=1206,20
PRIMER_LEFT_12_TM=59.186
PRIMER_RIGHT_12_TM=59.661
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=683
PRIMER_PAIR_13_PENALTY=1.153764
PRIMER_LEFT_13_PENALTY=0.814339
PRIMER_RIGHT_13_PENALTY=0.339425
PRIMER_LEFT_13_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_13_SEQUENCE=TCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_13=524,20
PRIMER_RIGHT_13=1208,20
PRIMER_LEFT_13_TM=59.186
PRIMER_RIGHT_13_TM=59.661
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=685
PRIMER_PAIR_14_PENALTY=1.221614
PRIMER_LEFT_14_PENALTY=0.066323
PRIMER_RIGHT_14_PENALTY=1.155291
PRIMER_LEFT_14_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_14_SEQUENCE=CTTTAGGGCTTCCCCTTTCT
PRIMER_LEFT_14=353,20
PRIMER_RIGHT_14=1207,20
PRIMER_LEFT_14_TM=60.066
PRIMER_RIGHT_14_TM=58.845
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=10.9000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=855
PRIMER_PAIR_15_PENALTY=1.245396
PRIMER_LEFT_15_PENALTY=0.814339
PRIMER_RIGHT_15_PENALTY=0.431057
PRIMER_LEFT_15_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_15_SEQUENCE=AAATTAGTTGGAGGCGGTGA
PRIMER_LEFT_15=524,20
PRIMER_RIGHT_15=1409,20
PRIMER_LEFT_15_TM=59.186
PRIMER_RIGHT_15_TM=59.569
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=886
PRIMER_PAIR_16_PENALTY=1.301579
PRIMER_LEFT_16_PENALTY=0.814339
PRIMER_RIGHT_16_PENALTY=0.487240
PRIMER_LEFT_16_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_16_SEQUENCE=ATCAATGGAGATCAAGCCGT
PRIMER_LEFT_16=524,20
PRIMER_RIGHT_16=1516,20
PRIMER_LEFT_16_TM=59.186
PRIMER_RIGHT_16_TM=59.513
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=11.1000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=993
PRIMER_PAIR_17_PENALTY=1.305715
PRIMER_LEFT_17_PENALTY=0.066323
PRIMER_RIGHT_17_PENALTY=1.239392
PRIMER_LEFT_17_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_17_SEQUENCE=TCGATTGAACTCTGGAAGTGG
PRIMER_LEFT_17=353,20
PRIMER_RIGHT_17=1329,21
PRIMER_LEFT_17_TM=60.066
PRIMER_RIGHT_17_TM=60.239
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=10.9000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=977
PRIMER_PAIR_18_PENALTY=1.306716
PRIMER_LEFT_18_PENALTY=0.231272
PRIMER_RIGHT_18_PENALTY=1.075444
PRIMER_LEFT_18_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_18_SEQUENCE=ATCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_18=338,20
PRIMER_RIGHT_18=1209,21
PRIMER_LEFT_18_TM=59.769
PRIMER_RIGHT_18_TM=59.925
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=872
PRIMER_PAIR_19_PENALTY=1.366622
PRIMER_LEFT_19_PENALTY=0.814339
PRIMER_RIGHT_19_PENALTY=0.552282
PRIMER_LEFT_19_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_19_SEQUENCE=TCCGGGCTAGACTTTTTCTG
PRIMER_LEFT_19=524,20
PRIMER_RIGHT_19=1126,20
PRIMER_LEFT_19_TM=59.186
PRIMER_RIGHT_19_TM=59.448
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=603
PRIMER_PAIR_20_PENALTY=1.386563
PRIMER_LEFT_20_PENALTY=0.231272
PRIMER_RIGHT_20_PENALTY=1.155291
PRIMER_LEFT_20_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_20_SEQUENCE=CTTTAGGGCTTCCCCTTTCT
PRIMER_LEFT_20=338,20
PRIMER_RIGHT_20=1207,20
PRIMER_LEFT_20_TM=59.769
PRIMER_RIGHT_20_TM=58.845
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=870
PRIMER_PAIR_21_PENALTY=1.470664
PRIMER_LEFT_21_PENALTY=0.231272
PRIMER_RIGHT_21_PENALTY=1.239392
PRIMER_LEFT_21_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_21_SEQUENCE=TCGATTGAACTCTGGAAGTGG
PRIMER_LEFT_21=338,20
PRIMER_RIGHT_21=1329,21
PRIMER_LEFT_21_TM=59.769
PRIMER_RIGHT_21_TM=60.239
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=992
PRIMER_PAIR_22_PENALTY=1.498107
PRIMER_LEFT_22_PENALTY=1.453276
PRIMER_RIGHT_22_PENALTY=0.044832
PRIMER_LEFT_22_SEQUENCE=AAAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_22_SEQUENCE=ATTAGTTGGAGGCGGTGATG
PRIMER_LEFT_22=523,21
PRIMER_RIGHT_22=1407,20
PRIMER_LEFT_22_TM=60.453
PRIMER_RIGHT_22_TM=59.955
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=885
PRIMER_PAIR_23_PENALTY=1.511252
PRIMER_LEFT_23_PENALTY=0.814339
PRIMER_RIGHT_23_PENALTY=0.696913
PRIMER_LEFT_23_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_23_SEQUENCE=TAGGGCTTCCCCTTTCTCTT
PRIMER_LEFT_23=524,20
PRIMER_RIGHT_23=1204,20
PRIMER_LEFT_23_TM=59.186
PRIMER_RIGHT_23_TM=59.303
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=681
PRIMER_PAIR_24_PENALTY=1.511252
PRIMER_LEFT_24_PENALTY=0.814339
PRIMER_RIGHT_24_PENALTY=0.696913
PRIMER_LEFT_24_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_24_SEQUENCE=TTAGGGCTTCCCCTTTCTCT
PRIMER_LEFT_24=524,20
PRIMER_RIGHT_24=1205,20
PRIMER_LEFT_24_TM=59.186
PRIMER_RIGHT_24_TM=59.303
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=682
PRIMER_PAIR_25_PENALTY=1.604499
PRIMER_LEFT_25_PENALTY=0.066323
PRIMER_RIGHT_25_PENALTY=1.538176
PRIMER_LEFT_25_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_25_SEQUENCE=TTAGGGCTTCCCCTTTCTCTT
PRIMER_LEFT_25=353,20
PRIMER_RIGHT_25=1205,21
PRIMER_LEFT_25_TM=60.066
PRIMER_RIGHT_25_TM=60.538
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=10.9000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=7.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=853
PRIMER_PAIR_26_PENALTY=1.604499
PRIMER_LEFT_26_PENALTY=0.066323
PRIMER_RIGHT_26_PENALTY=1.538176
PRIMER_LEFT_26_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_26_SEQUENCE=TTTAGGGCTTCCCCTTTCTCT
PRIMER_LEFT_26=353,20
PRIMER_RIGHT_26=1206,21
PRIMER_LEFT_26_TM=60.066
PRIMER_RIGHT_26_TM=60.538
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.9000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=854
PRIMER_PAIR_27_PENALTY=1.604499
PRIMER_LEFT_27_PENALTY=0.066323
PRIMER_RIGHT_27_PENALTY=1.538176
PRIMER_LEFT_27_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_27_SEQUENCE=CTTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_27=353,20
PRIMER_RIGHT_27=1207,21
PRIMER_LEFT_27_TM=60.066
PRIMER_RIGHT_27_TM=60.538
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=10.9000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=855
PRIMER_PAIR_28_PENALTY=1.604499
PRIMER_LEFT_28_PENALTY=0.066323
PRIMER_RIGHT_28_PENALTY=1.538176
PRIMER_LEFT_28_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_28_SEQUENCE=TCTTTAGGGCTTCCCCTTTCT
PRIMER_LEFT_28=353,20
PRIMER_RIGHT_28=1208,21
PRIMER_LEFT_28_TM=60.066
PRIMER_RIGHT_28_TM=60.538
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=10.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=856
PRIMER_PAIR_29_PENALTY=1.624288
PRIMER_LEFT_29_PENALTY=0.066323
PRIMER_RIGHT_29_PENALTY=1.557965
PRIMER_LEFT_29_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_29_SEQUENCE=TTTCCGGGCTAGACTTTTTC
PRIMER_LEFT_29=353,20
PRIMER_RIGHT_29=1128,20
PRIMER_LEFT_29_TM=60.066
PRIMER_RIGHT_29_TM=58.442
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=10.9000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=776
PRIMER_PAIR_30_PENALTY=1.685329
PRIMER_LEFT_30_PENALTY=0.066323
PRIMER_RIGHT_30_PENALTY=1.619006
PRIMER_LEFT_30_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_30_SEQUENCE=TTTCCGGGCTAGACTTTTTCT
PRIMER_LEFT_30=353,20
PRIMER_RIGHT_30=1128,21
PRIMER_LEFT_30_TM=60.066
PRIMER_RIGHT_30_TM=59.381
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=10.9000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=776
PRIMER_PAIR_31_PENALTY=1.693858
PRIMER_LEFT_31_PENALTY=1.354433
PRIMER_RIGHT_31_PENALTY=0.339425
PRIMER_LEFT_31_SEQUENCE=AGGTGAAGCTTATTGGGGAA
PRIMER_RIGHT_31_SEQUENCE=TTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_31=354,20
PRIMER_RIGHT_31=1206,20
PRIMER_LEFT_31_TM=58.646
PRIMER_RIGHT_31_TM=59.661
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.7000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=7.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=853
PRIMER_PAIR_32_PENALTY=1.696555
PRIMER_LEFT_32_PENALTY=1.357130
PRIMER_RIGHT_32_PENALTY=0.339425
PRIMER_LEFT_32_SEQUENCE=AGCTTATTGGGGAACGCAAC
PRIMER_RIGHT_32_SEQUENCE=TTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_32=360,20
PRIMER_RIGHT_32=1206,20
PRIMER_LEFT_32_TM=61.357
PRIMER_RIGHT_32_TM=59.661
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=847
PRIMER_PAIR_33_PENALTY=1.696555
PRIMER_LEFT_33_PENALTY=1.357130
PRIMER_RIGHT_33_PENALTY=0.339425
PRIMER_LEFT_33_SEQUENCE=AGCTTATTGGGGAACGCAAC
PRIMER_RIGHT_33_SEQUENCE=TCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_33=360,20
PRIMER_RIGHT_33=1208,20
PRIMER_LEFT_33_TM=61.357
PRIMER_RIGHT_33_TM=59.661
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=849
PRIMER_PAIR_34_PENALTY=1.701877
PRIMER_LEFT_34_PENALTY=0.814339
PRIMER_RIGHT_34_PENALTY=0.887538
PRIMER_LEFT_34_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_34_SEQUENCE=CGGTGATGATACGAGTGGAG
PRIMER_LEFT_34=524,20
PRIMER_RIGHT_34=1395,20
PRIMER_LEFT_34_TM=59.186
PRIMER_RIGHT_34_TM=59.112
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=872
PRIMER_PAIR_35_PENALTY=1.726905
PRIMER_LEFT_35_PENALTY=0.814339
PRIMER_RIGHT_35_PENALTY=0.912566
PRIMER_LEFT_35_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_35_SEQUENCE=AAAATTAGTTGGAGGCGGTG
PRIMER_LEFT_35=524,20
PRIMER_RIGHT_35=1410,20
PRIMER_LEFT_35_TM=59.186
PRIMER_RIGHT_35_TM=59.087
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=887
PRIMER_PAIR_36_PENALTY=1.726905
PRIMER_LEFT_36_PENALTY=0.814339
PRIMER_RIGHT_36_PENALTY=0.912566
PRIMER_LEFT_36_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_36_SEQUENCE=CAAAATTAGTTGGAGGCGGT
PRIMER_LEFT_36=524,20
PRIMER_RIGHT_36=1411,20
PRIMER_LEFT_36_TM=59.186
PRIMER_RIGHT_36_TM=59.087
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=11.1000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=888
PRIMER_PAIR_37_PENALTY=1.753339
PRIMER_LEFT_37_PENALTY=0.231272
PRIMER_RIGHT_37_PENALTY=1.522067
PRIMER_LEFT_37_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_37_SEQUENCE=AATCTTTAGGGCTTCCCCTTT
PRIMER_LEFT_37=338,20
PRIMER_RIGHT_37=1210,21
PRIMER_LEFT_37_TM=59.769
PRIMER_RIGHT_37_TM=59.478
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=873
PRIMER_PAIR_38_PENALTY=1.769448
PRIMER_LEFT_38_PENALTY=0.231272
PRIMER_RIGHT_38_PENALTY=1.538176
PRIMER_LEFT_38_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_38_SEQUENCE=TTAGGGCTTCCCCTTTCTCTT
PRIMER_LEFT_38=338,20
PRIMER_RIGHT_38=1205,21
PRIMER_LEFT_38_TM=59.769
PRIMER_RIGHT_38_TM=60.538
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=868
PRIMER_PAIR_39_PENALTY=1.769448
PRIMER_LEFT_39_PENALTY=0.231272
PRIMER_RIGHT_39_PENALTY=1.538176
PRIMER_LEFT_39_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_39_SEQUENCE=TTTAGGGCTTCCCCTTTCTCT
PRIMER_LEFT_39=338,20
PRIMER_RIGHT_39=1206,21
PRIMER_LEFT_39_TM=59.769
PRIMER_RIGHT_39_TM=60.538
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=869
PRIMER_PAIR_40_PENALTY=1.769448
PRIMER_LEFT_40_PENALTY=0.231272
PRIMER_RIGHT_40_PENALTY=1.538176
PRIMER_LEFT_40_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_40_SEQUENCE=CTTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_40=338,20
PRIMER_RIGHT_40=1207,21
PRIMER_LEFT_40_TM=59.769
PRIMER_RIGHT_40_TM=60.538
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=870
PRIMER_PAIR_41_PENALTY=1.769448
PRIMER_LEFT_41_PENALTY=0.231272
PRIMER_RIGHT_41_PENALTY=1.538176
PRIMER_LEFT_41_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_41_SEQUENCE=TCTTTAGGGCTTCCCCTTTCT
PRIMER_LEFT_41=338,20
PRIMER_RIGHT_41=1208,21
PRIMER_LEFT_41_TM=59.769
PRIMER_RIGHT_41_TM=60.538
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=871
PRIMER_PAIR_42_PENALTY=1.779938
PRIMER_LEFT_42_PENALTY=0.066323
PRIMER_RIGHT_42_PENALTY=1.713615
PRIMER_LEFT_42_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_42_SEQUENCE=CGATTGAACTCTGGAAGTGG
PRIMER_LEFT_42=353,20
PRIMER_RIGHT_42=1328,20
PRIMER_LEFT_42_TM=60.066
PRIMER_RIGHT_42_TM=58.286
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.9000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=976
PRIMER_PAIR_43_PENALTY=1.783639
PRIMER_LEFT_43_PENALTY=0.066323
PRIMER_RIGHT_43_PENALTY=1.717315
PRIMER_LEFT_43_SEQUENCE=CAGGTGAAGCTTATTGGGGA
PRIMER_RIGHT_43_SEQUENCE=TTCCGGGCTAGACTTTTTCTG
PRIMER_LEFT_43=353,20
PRIMER_RIGHT_43=1127,21
PRIMER_LEFT_43_TM=60.066
PRIMER_RIGHT_43_TM=60.717
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=10.9000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=775
PRIMER_PAIR_44_PENALTY=1.789237
PRIMER_LEFT_44_PENALTY=0.231272
PRIMER_RIGHT_44_PENALTY=1.557965
PRIMER_LEFT_44_SEQUENCE=ACTCTGTTTCGTTGCCAGGT
PRIMER_RIGHT_44_SEQUENCE=TTTCCGGGCTAGACTTTTTC
PRIMER_LEFT_44=338,20
PRIMER_RIGHT_44=1128,20
PRIMER_LEFT_44_TM=59.769
PRIMER_RIGHT_44_TM=58.442
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=7.3000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=791
PRIMER_PAIR_45_PENALTY=1.792701
PRIMER_LEFT_45_PENALTY=1.453276
PRIMER_RIGHT_45_PENALTY=0.339425
PRIMER_LEFT_45_SEQUENCE=AAAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_45_SEQUENCE=TTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_45=523,21
PRIMER_RIGHT_45=1206,20
PRIMER_LEFT_45_TM=60.453
PRIMER_RIGHT_45_TM=59.661
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=684
PRIMER_PAIR_46_PENALTY=1.792701
PRIMER_LEFT_46_PENALTY=1.453276
PRIMER_RIGHT_46_PENALTY=0.339425
PRIMER_LEFT_46_SEQUENCE=AAAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_46_SEQUENCE=TCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_46=523,21
PRIMER_RIGHT_46=1208,20
PRIMER_LEFT_46_TM=60.453
PRIMER_RIGHT_46_TM=59.661
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=686
PRIMER_PAIR_47_PENALTY=1.815771
PRIMER_LEFT_47_PENALTY=0.814339
PRIMER_RIGHT_47_PENALTY=1.001431
PRIMER_LEFT_47_SEQUENCE=AAGGATTTATGTTGCGAGCC
PRIMER_RIGHT_47_SEQUENCE=CAACGAGAGAGATGGCAAAA
PRIMER_LEFT_47=524,20
PRIMER_RIGHT_47=1426,20
PRIMER_LEFT_47_TM=59.186
PRIMER_RIGHT_47_TM=58.999
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=903
PRIMER_PAIR_48_PENALTY=1.835440
PRIMER_LEFT_48_PENALTY=1.496015
PRIMER_RIGHT_48_PENALTY=0.339425
PRIMER_LEFT_48_SEQUENCE=GGTGAAGCTTATTGGGGAAC
PRIMER_RIGHT_48_SEQUENCE=TTTAGGGCTTCCCCTTTCTC
PRIMER_LEFT_48=355,20
PRIMER_RIGHT_48=1206,20
PRIMER_LEFT_48_TM=58.504
PRIMER_RIGHT_48_TM=59.661
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=852
PRIMER_PAIR_49_PENALTY=1.835440
PRIMER_LEFT_49_PENALTY=1.496015
PRIMER_RIGHT_49_PENALTY=0.339425
PRIMER_LEFT_49_SEQUENCE=GGTGAAGCTTATTGGGGAAC
PRIMER_RIGHT_49_SEQUENCE=TCTTTAGGGCTTCCCCTTTC
PRIMER_LEFT_49=355,20
PRIMER_RIGHT_49=1208,20
PRIMER_LEFT_49_TM=58.504
PRIMER_RIGHT_49_TM=59.661
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=854
=
SEQUENCE_ID=MAGIC.446_2_7447999_7448097_oy-0_no-0_157298_1805498_TRUE
SEQUENCE_TARGET=950,198
SEQUENCE_TEMPLATE=CAATGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAATTTGCTTTATGACAAGTTCTTAGACTNGGATTTAGTTTGTTTTTGGAGTTGGTAAAGACGATAACTANAGTTGATTTAAGGGACGATGGAAACGTTTTAGTTATTATAGTGAGAGACGTAATTCTATNAAAGTGTTATTATTTCTAGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_4208818_4208984_wil-2_ws-0_13364_107728_TRUE
SEQUENCE_TARGET=950,266
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACTTTACAAAANTAAATACAAANCANTTGNAATATTTATTNTTNGAAAAANGACAATNGTTNCANANTTTTTNTCTCTTCTCTCTCATCAAAAACNCNAAAAANNANATCTAAGTTTTTCTTTTNNTTTCTCTTCTTAGATCTTAAAAGCTGNNNNNNNNNNAGATTTCGATTCAGTNTNNNCNNTAATTTCATAANGTTGAAANNNNNNNNNNTTTTTNCTGGNTTTCTTCTTCTCTTATTGTATCCATCATNGTCTTAGTCAAATTNNGTCTCTTTACAAATTTTANATTCGTCCACNAAGTGTTNGTTNAAATTNCTNNNNNNGAGAGAGAGAGAGTTGAATTAAAGAANCCAAACATATATTCTTACTATAAATTTGTAACTNAANNNNAGNTTTTGTGCGATTTTCATTAAAACACATNAGCAAANNNNNNNNANNTNGAAGAAATANGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAGGCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4551614_4552108_kn-0_sf-2_28227_20461_TRUE
SEQUENCE_TARGET=950,594
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCAAGGTTCTAACGCTATGACAGAATTCCTCTAACCCGNGAAACTAAAATGACTCANACCGCGAAACACTTAAANNANNNNNNTTTTTTCTGNGAACANACATATTTTCCCTCTGAGCTCNAGAAAACCCTAAGNAAATAAAACCCTCAAACGGACGACTCGAATCCTGGAGCCACCGNTTNNNNTCGCCACCGCCGTTGTTGAAGTTTGCCGCCGTCAAAGCAGGAGCCGCAACAACAATCCCAATTTCGGCCCTAATTGTCCGATTAAACCATCTCTCTGAATCCCAAATCGAAGGCATGTCTCTCTGAATCTGTAGATTTATTTCATCTCTTTCGTTAACCTAGTTAGNTTTCTTTTAGCTGTCGTTNATCCNTTGAACTGTTTTGGTTCTTAATCACNGGATTTATGGGTTTCTCTGATTCGNTNNNNNNNNNNNNNNNTCTTTTGCATGTCCGTGTTCTTTGCAAAATTAGAANTTTATGCTTATGTGGTTTGATTTAGGCGTAGCTGGATAAATAGAATGCACAATTTTGCTTTCGGAAANNNNNNNNTTTTAGATTNTANCAAAACTTCTCGAGATTTACAAGTGTTTGTTGACATTCTANTACAGCTNTTTGTATTGACTTGTTCTTCGAATCTTTGGATCTTTGAGTGCTTGTAATGACATGGTCTCTGAATGTTTAGATTATTTCCACAACTTNNNNNNNNNNNNNNNNNNNNNNCAAACACATGAAACTAGATATACTCATTCTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_15091986_15092302_no-0_oy-0_85762_37247_TRUE
SEQUENCE_TARGET=950,416
SEQUENCE_TEMPLATE=TTCATGAGTTAACTCTTTCCCNTAAGATACATTCTTTTTGCAACCTAACTTGGNGTTCGGACACGGGATCAAGATCGATTCAAGNACACTCTCCATTGCTCTGCAGCGATTGTNGCCAACAGGCNAAGNACANGNAGGGNNNNNNNNNNNNNNNNTGGGACAGCAAGNAGAACAAGCCAAATGTCCATTATCACACTGAAATTCAAAACCAATAACTTGTGATCAAAAGCTATAACAACGTCGAGNTAATANTCATCGANTCGACCNAGGATAATNNTTCTCTCCCNAAATGATTCTCCCATATAANTCATGATATAGACAAGTTATANACATATTTTCAATAACTATATACAACTGCANACATGAACATAACTTACNAAAGTTCACTAGAATTCTATAAAATCATCACAAGCTAAAGCTTAANGACACTCTTGAAATCTNGTAANTAGTAAATGGGCACAATTCCAACTTCTNCTCTCTGNAATTTCNTTAACATAACTTTCAAAANTTCACTAGAATCNTCAAAAGNTAAGGCTANTCTTAGAATCNNGTAAATGGCNACAGTTCCAACTATNTNCAGAGTTCTAACCNAAACAGAAACTNTCTGNNANATACGAAATGATTCANTTTNAGAAATTACGAAACCTGGAAGATGGNAATTGTGAAAGCTTCGTAGCAAATGGGACANTCGAGAATCTCCAGATCCATTAGCATNGTCGAGCGTTTCTTAGCGTCTCTCTTNGCTGCATCACTAGAAGAAAGTTGTCTCTTTTGCGAGAGAATGCTGTNNNNNNNNNNNNNNNNNNCGGNAGNTTCCAAAATCGCGGCANCAACCATGTCGGAGAGATGACTAAACCNTAAATTCGCTTTTANNNTTNTCGNAAACTGAGAGAGGNAANTAGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTTACAGAGAAANNAGNCAATGGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAGGAAATGATTNTTTGAAAGATTCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGATNTATAGGTTAGTATATGCTTAAACCAATATAATTTCAATAACCATANCGTTTNCAGAATTAANAATTTATGNGTTANNTAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNTNTTAGCATATTCATAGAGCATTCACCCNAATNATGAATTTGTGTNNNNNNNNNANTTTTTNAATTGTTANTTTTAATAANTAAAAATANNATAANNANTTGAATNCTNTTTTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_16481201_16481671_zu-0_wil-2_157528_208568_TRUE
SEQUENCE_TARGET=950,570
SEQUENCE_TEMPLATE=TTTATAACTTAACTTAATTAGNAAATACGTTTCATTGACTACTTGTACNATATCTCTATCAAGTCGAAACATTNTATAACTTTANAGAATTAACTATTACGTACGAATGGAATAAAATGCAGAAGAACAACGTGTCTCTTTCACCGCCGGCTTAGCGTCCATTTNGCGCATCCGTAGCTAATGTACAACATGCATGTGTCNTACCTAATGCATTGATGTTTCCAGCTTANTATAATANTTTGAAAATATTGTTGATCNTTTTGNTTAATGGTTATACTNNTACATTATATNTAAATATTGTTAATTTACNAGAATATCTTCTTATTNTTTTTNTTTTTTTGTTANAGAGTCATCNAAGTACAAGAATATCTTNATGGAGAGACAAATGTTTGACANTTGACATCAACCAAAGGTCAATGAGACTATGANATCGTACGGTNCATTGTAAAAAGCTCATTCTTCTAAANATAATTGCAGTTTGTCTTNTGTGTATTCCATGTGGTCGACTCATCAAATTAATTTTANTNTTGGAAATNNTTNTATCAACTNTANNNNNNNNNNNNNNNNNTNTNTTTTNNNNNNAATNNNNNNNNNNTACCATAAGATATGAAAATCTCTTGATNNTTANTTATTTTTGNGGGTTTNAAACAAGNTCGNTCATTTCTTATTTGGAACGTAAANTCATATTTTANANAAAAAACTAAAATTNCAGANAGCAAAATTTTGGNAANCAAAAAGANCGAAACAAGNAACATCAGNTTTTNAAAGTTATCAAATGAATTGAATGTAGCGATAAGTTNTCTTTCTNNCATATTCNTCGATCNCCGTACCATCTAGATNATCTTTNNNNATTNGNNNNNNNNNNNNTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATANTTTTAGTTANTCNATACAGNTTGATGCATTTGTNAAAAAAANTTATATNATTTTGTCATTTANTTNNNTTAATANTTTGTAGTGNATTATAGGTAAAGAACNTGTTNNTTTAATTCTTTTGNNTTNNTTTTTTTNNGTGCATTTTACGTAANCANGTAAAGAAAAGTCCNATCTTTATAGGTAAAGAAAATNCCAATAATGCGGGANAAGTCAAACAAAAACTCNTAACCATTGATCTTTCATCGGGATGGTCTTTGACTTGTATNAAGTGNACGTTTTTCTCTTAAAAGTAGTTGCAACTTNCTAGATTTCTANTGATATTATATTCGATTCAAAAAGCTAATGCACAACAACAAGATCTAACTAATTTATATCACATTTGACAATGTTATCAAAAACATACATATAAACCATCGTTAGCTCCAGTATTTTAGAGAAAAACTTGGTTGTTTCANGAGATTTCTAGNTAATTGTCCTTNTAGCATANGCTAGTGANATATATANTCCTATATTGTAAAAGTTTCANAAGTGATCNTGNAAATCTACNNNNNNTCNTGTTTNATATATGGTCTAAAGTGTNATNTNNNTNNTTTTTATCAGACAAANAANGTCAGCTACTTACAAACTTTTGATAATTCATAATCGCTGCTTATATTGCGGCAAGTTTCACGTCATTATCAACGTGGGGATGATTCAGCAGAAAANTTAGAAAANTGGGTTGATGCTTATCGTCGTGGTTTCTGATAGGCGTACTTTACATTAATGATAATCTCTNGGACTTATATTAATAATTTCAAAGTCGTACCNATNACTTGTACTATTTAATACCGTACAGATCACAGCTGTTTCTTATGCTAATGACGTGGAAGATGTACGGGCAGCTGACTATGATCCAACACATCTGAAATTGATACACGTCTCTTTTTCTTGCCAACTTTGTACTTATGTGTATACATAGATCTCTGTACTTTTTTGCTGCTTCACATGTTAATGTTAGGTACACTTTTCGTNGATCACTGTAAAACTTATTAAACTGATTTTATGTTAAACATTGTAAATTAATTAGTTTGCGTAAGATCTTTTNCATAATATANTTGCTTAGGAAGGTTATAAAATCTTTCAGCTCATGTTATTTGCCCCNAACTTCGGTGGCTATGCCTAGAGATTTTTAGGTTATATAAATACAAACAATGGNTTTGACCAGTATTTTCTGTACTACTTTAGAAATTATTAATCACAAAAACGTCACTAAAANCCTTATTNCANTTTTTNTTTATTCCGTTATTTTATACTATTTTAATATCACTTANCNTTTTNTTATCTGTTNNNAAACTTGTACCGTTTTCTAATTTACGAATTTAATCTAAATATTACAGAGAATATGTANNTACAAATATATATTTCATAAACATANTTAACATGCTAGAATGGGTATGAATTCATGTTTGTTGGTCCATCATGTCATGGTAGTCTAAACTAANCTAACACTTTGATCATGGTCAATAAAGTATAAAACTTAAAATATATGACTATTANT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13159582_13159663_can-0_wu-0_6470_13069_TRUE
SEQUENCE_TARGET=950,181
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4722510_4722576_wu-0_ler-0_9861_16546_TRUE
SEQUENCE_TARGET=950,166
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTNTACNGGTTNATAACNGATTTANAAANTAATNNNNNNNNNNNNNNNNNNNNNNNNTNTAANTNTTGAATTAAATNTATGTNATCAGNTTATNAATATANNTNGTTANTTNGCANNGNANAGATNAACTNAAATAAACCNANNTTNNNAAGCANTCCAANNGTNGNGNCTCTANTNNTANNNACACTCANACANACTGGANCNAACNGNATGCTAGNGNNGNNNAGANNNNNNNNNNNNNNNNNNNNGACAANNTNTTTTGTCTNTTATGNGTTTAGGNTTTNGGNGNNNNNNNNNNNNNNNNNNNNATTAANCGTACAACATNATTATATANATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCCNAATTACNAAACTCTCNACAANNTTNATTGTTTAATAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANGTNATNNATTATAAGANGNTTNTANCAAAACATTATAATCANCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNATANAANTTAAACTAGAGTNAGTACAAATGATTTNANGANATTCCCAANNANGTNCCNCTTGTANNNGAATCATCTATTCNNNTAATCCTTTNNNNNNNNNNTCTNGATCTTAGCATAAGGTAAGAAANNGTGTCAACCTTATTANACTNAATCATATTNATCNAACTCTGANTTNNNNTGANAAANAAACGATTGACTNTCAATCNNTTTNAGCACGGNCATGCATTTCTCNNTATCAANTNNTCGATAGGCCCAAAGATATTTANCTCCTNTTATATAGGAGGGANAAATCCCATNTTGTTCCATCCNTGTTCCTTATANAATTCATNATACNNCCAATNANTNTCTTTANAATCANCTGTTACNGNTGANGTTTGANANAGTCNANACNNACCAATCCATGNATAANANAACATGACNAACTNAGGTCAAAGGACTANACTCTATAGTNGNGANNNGAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4551614_4551668_kn-0_wu-0_22182_46770_TRUE
SEQUENCE_TARGET=950,154
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCAAGGTTCTAACGCTATGACAGAATTCCTCTAACCCGNGAAACTAAAATGACTCANACCGCGAAACACTTAAANNANNNNNNTTTTTTCTGNGAACANACATATTTTCCCTCTGAGCTCNAGAAAACCCTAAGNAAATAAAACCCTCAAACGGACGACTCGAATCCTGGAGCCACCGNTTNNNNTCGCCACCGCCGTTGTTGAAGTTTGCCGCCGTCAAAGCAGGAGCCGCAACAACAATCCCAATTTCGGCCCTAATTGTCCGATTAAACCATCTCTCTGAATCCCAAATCGAAGGCATGTCTCTCTGAATCTGTAGATTTATTTCATCTCTTTCGTTAACCTAGTTAGNTTTCTTTTAGCTGTCGTTNATCCNTTGAACTGTTTTGGTTCTTAATCACNGGATTTATGGGTTTCTCTGATTCGNTNNNNNNNNNNNNNNNTCTTTTGCATGTCCGTGTTCTTTGCAAAATTAGAANTTTATGCTTATGTGGTTTGATTTAGGCGTAGCTGGATAAATAGAATGCACAATTTTGCTTTCGGAAANNNNNNNNTTTTAGATTNTANCAAAACTTCTCGAGATTTACAAGTGTTTGTTGACATTCTANTACAGCTNTTTGTATTGACTTGTTCTTCGAATCTTTGGATCTTTGAGTGCTTGTAATGACATGGTCTCTGAATGTTTAGATTATTTCCACAACTTNNNNNNNNNNNNNNNNNNNNNNCAAACACATGAAACTAGATATACTCATTCTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_8157950_8158032_can-0_ws-0_1762_126238_TRUE
SEQUENCE_TARGET=950,182
SEQUENCE_TEMPLATE=CTAAGTTCTAATCTACAACTACAAGNGGTACTTGCGCTTTAACACATCTGTTTATGTTANTNTATTNATTTATAGNAAAGACTGGCTCTGCTTCTTGTAGAATGTTCTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANCAACAGAGCAACAACTGTANGCAAAACNAAACAAAAAGNAANANNNNNNGNCNNNNNNNANNNNGTCTAATATCTACTATATGAGTATGACATTCCCATTTGGTTGTATATTGCATAGGTTCGGAACTTGTGAAAATTGAGCCAAACAAANGGTGAACGAAGAACGGTACGGAGTTATTAATAGTNTCTGGAAGTAATGTTTTACTTCACATNGTTTCTTCTTGTATCTTTTGTTTTATTCANAAGAAAAAGTCTGCGTCTGTGAAAANAATGGTAGATGAAGAANTTGTGGACAGATTTGTATTAGTAGAGAAGAGGTAGATTCNAAANNAANTATATATGNATCTTATAGAAGTCTTTGTGTGATGTTTTTGGTATATTCAGGTCNCACTTGTTGNTCTAGTGTGTGACAAAGATGTNTCNAAANTCACANAANTTAGATTTACTCNTTGATGANGGAACAGGACGAATTGATTGNAAAANATGGTAATTAACACACATANACTATACCNTTTTGANATATGGAAGAGTTNTTTTNAGGTACAATNGTANNTTNNTNNNNNGNNNANTNACCCACTTAGTGACACATCCTTCATGATANCAATGCTTACAATAAGGCATCTGGCAANAATTCTCANATCTCGACATATTCTCCAAACAGATNGCACAAGTCTCCGATTCTTCTTCTTCTNGGTCCAAATCTNTNGCCGACGGAGAAGAAATACAAGGCACAACACTGTATAACTTGTGTGTCAATCTGACAGAGACTATCATGAATAAAGGTTGTTGACGTTGTTGTGGCAAGTAAAAACTTTGACTTCTTNGCAGNTGGGCTTCGANAATCTTAGAACATATTTGTNCGGACANGTCACGATNGTGGAGTTGGNNNNNNNNNCGTTGATNGACGTAGTTCCGTTAGTGTAATTTGCTANTTAGTGTAATANCCAAAAATANGAATATTNTTACTAGAATANAGANAGTGCGAGATNNNNNNNNNNNNNNNNNNATGAAAATGAAAAGTAGNATTGTTTGGGAATTNGGATTGCAGTCNNAGCTAAGAGATCTGCATGAAGGTTGGNTTAATCTTCNACGAATCCAGGAGGTTTGCGTCATTTGTACATAATNAGTTGCAAGTTATGAGTGAAATTGGACTTATATCNCAGANTCAGANACCCATTTTATACACATTANNNACACAAAATAGATATTAGAATTATATGTTGCACCTATCATAACCCGAAAACCAAGAAGGCTCAGCAAGATNTGAAAACTCAGTTTCNTTTTTGTAAAGCGGCTCAAACTCATCTCTCCATTGCTGCTCACTACTTGCATTTACTGATGGTTCCACGGCTTGATCACATTTGGTAGAGCATTCATTGTGAATATGAGATTGTGGGACGAGTTGGTTAAGTGTGTTGATTTCGTTTTCATGTTTCTTCTTCAGCTTGTCAATCTGTCTATATGCTTCTGCAGCTTCATACTCTGCATCCATCGCACGTTTCTGTTGACACAAGCCACATAGATTTTATGTAAGAACATGCATGCATAATAATAAAAGNAACAGACTTATTCAAGGATGTGACCTGACATGTTTAGAAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.322071
PRIMER_LEFT_0_PENALTY=0.265715
PRIMER_RIGHT_0_PENALTY=0.056356
PRIMER_LEFT_0_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_0_SEQUENCE=GCCACAACAACGTCAACAAC
PRIMER_LEFT_0=636,20
PRIMER_RIGHT_0=1283,20
PRIMER_LEFT_0_TM=59.734
PRIMER_RIGHT_0_TM=60.056
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=648
PRIMER_PAIR_1_PENALTY=0.368369
PRIMER_LEFT_1_PENALTY=0.265715
PRIMER_RIGHT_1_PENALTY=0.102654
PRIMER_LEFT_1_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_1_SEQUENCE=CCACAACAACGTCAACAACC
PRIMER_LEFT_1=636,20
PRIMER_RIGHT_1=1282,20
PRIMER_LEFT_1_TM=59.734
PRIMER_RIGHT_1_TM=59.897
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=647
PRIMER_PAIR_2_PENALTY=0.464216
PRIMER_LEFT_2_PENALTY=0.265715
PRIMER_RIGHT_2_PENALTY=0.198500
PRIMER_LEFT_2_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_2_SEQUENCE=ACTTGCCACAACAACGTCAA
PRIMER_LEFT_2=636,20
PRIMER_RIGHT_2=1287,20
PRIMER_LEFT_2_TM=59.734
PRIMER_RIGHT_2_TM=60.199
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=652
PRIMER_PAIR_3_PENALTY=0.668177
PRIMER_LEFT_3_PENALTY=0.265715
PRIMER_RIGHT_3_PENALTY=0.402462
PRIMER_LEFT_3_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_3_SEQUENCE=ACAAATGACGCAAACCTCCT
PRIMER_LEFT_3=636,20
PRIMER_RIGHT_3=1598,20
PRIMER_LEFT_3_TM=59.734
PRIMER_RIGHT_3_TM=59.598
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=963
PRIMER_PAIR_4_PENALTY=1.335609
PRIMER_LEFT_4_PENALTY=1.279252
PRIMER_RIGHT_4_PENALTY=0.056356
PRIMER_LEFT_4_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_4_SEQUENCE=GCCACAACAACGTCAACAAC
PRIMER_LEFT_4=638,20
PRIMER_RIGHT_4=1283,20
PRIMER_LEFT_4_TM=58.721
PRIMER_RIGHT_4_TM=60.056
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=646
PRIMER_PAIR_5_PENALTY=1.335609
PRIMER_LEFT_5_PENALTY=1.279252
PRIMER_RIGHT_5_PENALTY=0.056356
PRIMER_LEFT_5_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_5_SEQUENCE=GCCACAACAACGTCAACAAC
PRIMER_LEFT_5=637,20
PRIMER_RIGHT_5=1283,20
PRIMER_LEFT_5_TM=58.721
PRIMER_RIGHT_5_TM=60.056
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=647
PRIMER_PAIR_6_PENALTY=1.381906
PRIMER_LEFT_6_PENALTY=1.279252
PRIMER_RIGHT_6_PENALTY=0.102654
PRIMER_LEFT_6_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_6_SEQUENCE=CCACAACAACGTCAACAACC
PRIMER_LEFT_6=638,20
PRIMER_RIGHT_6=1282,20
PRIMER_LEFT_6_TM=58.721
PRIMER_RIGHT_6_TM=59.897
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=645
PRIMER_PAIR_7_PENALTY=1.381906
PRIMER_LEFT_7_PENALTY=1.279252
PRIMER_RIGHT_7_PENALTY=0.102654
PRIMER_LEFT_7_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_7_SEQUENCE=CCACAACAACGTCAACAACC
PRIMER_LEFT_7=637,20
PRIMER_RIGHT_7=1282,20
PRIMER_LEFT_7_TM=58.721
PRIMER_RIGHT_7_TM=59.897
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=646
PRIMER_PAIR_8_PENALTY=1.467545
PRIMER_LEFT_8_PENALTY=0.265715
PRIMER_RIGHT_8_PENALTY=1.201830
PRIMER_LEFT_8_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_8_SEQUENCE=TACTTGCCACAACAACGTCAA
PRIMER_LEFT_8=636,20
PRIMER_RIGHT_8=1288,21
PRIMER_LEFT_8_TM=59.734
PRIMER_RIGHT_8_TM=60.202
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.9000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=653
PRIMER_PAIR_9_PENALTY=1.467545
PRIMER_LEFT_9_PENALTY=0.265715
PRIMER_RIGHT_9_PENALTY=1.201830
PRIMER_LEFT_9_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_9_SEQUENCE=TTACTTGCCACAACAACGTCA
PRIMER_LEFT_9=636,20
PRIMER_RIGHT_9=1289,21
PRIMER_LEFT_9_TM=59.734
PRIMER_RIGHT_9_TM=60.202
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=654
PRIMER_PAIR_10_PENALTY=1.477753
PRIMER_LEFT_10_PENALTY=1.279252
PRIMER_RIGHT_10_PENALTY=0.198500
PRIMER_LEFT_10_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_10_SEQUENCE=ACTTGCCACAACAACGTCAA
PRIMER_LEFT_10=638,20
PRIMER_RIGHT_10=1287,20
PRIMER_LEFT_10_TM=58.721
PRIMER_RIGHT_10_TM=60.199
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=650
PRIMER_PAIR_11_PENALTY=1.477753
PRIMER_LEFT_11_PENALTY=1.279252
PRIMER_RIGHT_11_PENALTY=0.198500
PRIMER_LEFT_11_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_11_SEQUENCE=ACTTGCCACAACAACGTCAA
PRIMER_LEFT_11=637,20
PRIMER_RIGHT_11=1287,20
PRIMER_LEFT_11_TM=58.721
PRIMER_RIGHT_11_TM=60.199
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=651
PRIMER_PAIR_12_PENALTY=1.508791
PRIMER_LEFT_12_PENALTY=0.265715
PRIMER_RIGHT_12_PENALTY=1.243076
PRIMER_LEFT_12_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_12_SEQUENCE=TACTTGCCACAACAACGTCA
PRIMER_LEFT_12=636,20
PRIMER_RIGHT_12=1288,20
PRIMER_LEFT_12_TM=59.734
PRIMER_RIGHT_12_TM=58.757
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=653
PRIMER_PAIR_13_PENALTY=1.599057
PRIMER_LEFT_13_PENALTY=1.542700
PRIMER_RIGHT_13_PENALTY=0.056356
PRIMER_LEFT_13_SEQUENCE=GAACGAAGAACGGTACGGAGT
PRIMER_RIGHT_13_SEQUENCE=GCCACAACAACGTCAACAAC
PRIMER_LEFT_13=636,21
PRIMER_RIGHT_13=1283,20
PRIMER_LEFT_13_TM=60.543
PRIMER_RIGHT_13_TM=60.056
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=648
PRIMER_PAIR_14_PENALTY=1.642491
PRIMER_LEFT_14_PENALTY=0.265715
PRIMER_RIGHT_14_PENALTY=1.376776
PRIMER_LEFT_14_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_14_SEQUENCE=TACAAATGACGCAAACCTCCT
PRIMER_LEFT_14=636,20
PRIMER_RIGHT_14=1599,21
PRIMER_LEFT_14_TM=59.734
PRIMER_RIGHT_14_TM=59.623
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=964
PRIMER_PAIR_15_PENALTY=1.645354
PRIMER_LEFT_15_PENALTY=1.542700
PRIMER_RIGHT_15_PENALTY=0.102654
PRIMER_LEFT_15_SEQUENCE=GAACGAAGAACGGTACGGAGT
PRIMER_RIGHT_15_SEQUENCE=CCACAACAACGTCAACAACC
PRIMER_LEFT_15=636,21
PRIMER_RIGHT_15=1282,20
PRIMER_LEFT_15_TM=60.543
PRIMER_RIGHT_15_TM=59.897
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=647
PRIMER_PAIR_16_PENALTY=1.681714
PRIMER_LEFT_16_PENALTY=1.279252
PRIMER_RIGHT_16_PENALTY=0.402462
PRIMER_LEFT_16_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_16_SEQUENCE=ACAAATGACGCAAACCTCCT
PRIMER_LEFT_16=638,20
PRIMER_RIGHT_16=1598,20
PRIMER_LEFT_16_TM=58.721
PRIMER_RIGHT_16_TM=59.598
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=961
PRIMER_PAIR_17_PENALTY=1.681714
PRIMER_LEFT_17_PENALTY=1.279252
PRIMER_RIGHT_17_PENALTY=0.402462
PRIMER_LEFT_17_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_17_SEQUENCE=ACAAATGACGCAAACCTCCT
PRIMER_LEFT_17=637,20
PRIMER_RIGHT_17=1598,20
PRIMER_LEFT_17_TM=58.721
PRIMER_RIGHT_17_TM=59.598
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=962
PRIMER_PAIR_18_PENALTY=1.741201
PRIMER_LEFT_18_PENALTY=1.542700
PRIMER_RIGHT_18_PENALTY=0.198500
PRIMER_LEFT_18_SEQUENCE=GAACGAAGAACGGTACGGAGT
PRIMER_RIGHT_18_SEQUENCE=ACTTGCCACAACAACGTCAA
PRIMER_LEFT_18=636,21
PRIMER_RIGHT_18=1287,20
PRIMER_LEFT_18_TM=60.543
PRIMER_RIGHT_18_TM=60.199
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=652
PRIMER_PAIR_19_PENALTY=1.864183
PRIMER_LEFT_19_PENALTY=0.265715
PRIMER_RIGHT_19_PENALTY=1.598468
PRIMER_LEFT_19_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_19_SEQUENCE=CAAATGACGCAAACCTCCTG
PRIMER_LEFT_19=636,20
PRIMER_RIGHT_19=1597,20
PRIMER_LEFT_19_TM=59.734
PRIMER_RIGHT_19_TM=61.598
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=962
PRIMER_PAIR_20_PENALTY=1.945162
PRIMER_LEFT_20_PENALTY=1.542700
PRIMER_RIGHT_20_PENALTY=0.402462
PRIMER_LEFT_20_SEQUENCE=GAACGAAGAACGGTACGGAGT
PRIMER_RIGHT_20_SEQUENCE=ACAAATGACGCAAACCTCCT
PRIMER_LEFT_20=636,21
PRIMER_RIGHT_20=1598,20
PRIMER_LEFT_20_TM=60.543
PRIMER_RIGHT_20_TM=59.598
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=963
PRIMER_PAIR_21_PENALTY=2.081496
PRIMER_LEFT_21_PENALTY=1.882995
PRIMER_RIGHT_21_PENALTY=0.198500
PRIMER_LEFT_21_SEQUENCE=ATGACATTCCCATTTGGTTG
PRIMER_RIGHT_21_SEQUENCE=ACTTGCCACAACAACGTCAA
PRIMER_LEFT_21=568,20
PRIMER_RIGHT_21=1287,20
PRIMER_LEFT_21_TM=58.117
PRIMER_RIGHT_21_TM=60.199
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=720
PRIMER_PAIR_22_PENALTY=2.155646
PRIMER_LEFT_22_PENALTY=0.265715
PRIMER_RIGHT_22_PENALTY=1.889931
PRIMER_LEFT_22_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_22_SEQUENCE=CCACAACAACGTCAACAACCT
PRIMER_LEFT_22=636,20
PRIMER_RIGHT_22=1282,21
PRIMER_LEFT_22_TM=59.734
PRIMER_RIGHT_22_TM=60.890
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.9000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=647
PRIMER_PAIR_23_PENALTY=2.292459
PRIMER_LEFT_23_PENALTY=0.265715
PRIMER_RIGHT_23_PENALTY=2.026744
PRIMER_LEFT_23_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_23_SEQUENCE=CACAACAACGTCAACAACCTTT
PRIMER_LEFT_23=636,20
PRIMER_RIGHT_23=1281,22
PRIMER_LEFT_23_TM=59.734
PRIMER_RIGHT_23_TM=59.973
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.9000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=646
PRIMER_PAIR_24_PENALTY=2.372390
PRIMER_LEFT_24_PENALTY=2.316034
PRIMER_RIGHT_24_PENALTY=0.056356
PRIMER_LEFT_24_SEQUENCE=GGAACTTGTGAAAATTGAGCC
PRIMER_RIGHT_24_SEQUENCE=GCCACAACAACGTCAACAAC
PRIMER_LEFT_24=604,21
PRIMER_RIGHT_24=1283,20
PRIMER_LEFT_24_TM=58.684
PRIMER_RIGHT_24_TM=60.056
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=680
PRIMER_PAIR_25_PENALTY=2.418687
PRIMER_LEFT_25_PENALTY=2.316034
PRIMER_RIGHT_25_PENALTY=0.102654
PRIMER_LEFT_25_SEQUENCE=GGAACTTGTGAAAATTGAGCC
PRIMER_RIGHT_25_SEQUENCE=CCACAACAACGTCAACAACC
PRIMER_LEFT_25=604,21
PRIMER_RIGHT_25=1282,20
PRIMER_LEFT_25_TM=58.684
PRIMER_RIGHT_25_TM=59.897
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=679
PRIMER_PAIR_26_PENALTY=2.467088
PRIMER_LEFT_26_PENALTY=0.265715
PRIMER_RIGHT_26_PENALTY=2.201373
PRIMER_LEFT_26_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_26_SEQUENCE=TGCCACAACAACGTCAACAA
PRIMER_LEFT_26=636,20
PRIMER_RIGHT_26=1284,20
PRIMER_LEFT_26_TM=59.734
PRIMER_RIGHT_26_TM=62.201
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=649
PRIMER_PAIR_27_PENALTY=2.467088
PRIMER_LEFT_27_PENALTY=0.265715
PRIMER_RIGHT_27_PENALTY=2.201373
PRIMER_LEFT_27_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_27_SEQUENCE=TTGCCACAACAACGTCAACA
PRIMER_LEFT_27=636,20
PRIMER_RIGHT_27=1285,20
PRIMER_LEFT_27_TM=59.734
PRIMER_RIGHT_27_TM=62.201
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=650
PRIMER_PAIR_28_PENALTY=2.481083
PRIMER_LEFT_28_PENALTY=1.279252
PRIMER_RIGHT_28_PENALTY=1.201830
PRIMER_LEFT_28_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_28_SEQUENCE=TACTTGCCACAACAACGTCAA
PRIMER_LEFT_28=638,20
PRIMER_RIGHT_28=1288,21
PRIMER_LEFT_28_TM=58.721
PRIMER_RIGHT_28_TM=60.202
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=651
PRIMER_PAIR_29_PENALTY=2.481083
PRIMER_LEFT_29_PENALTY=1.279252
PRIMER_RIGHT_29_PENALTY=1.201830
PRIMER_LEFT_29_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_29_SEQUENCE=TTACTTGCCACAACAACGTCA
PRIMER_LEFT_29=638,20
PRIMER_RIGHT_29=1289,21
PRIMER_LEFT_29_TM=58.721
PRIMER_RIGHT_29_TM=60.202
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=8.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=652
PRIMER_PAIR_30_PENALTY=2.481083
PRIMER_LEFT_30_PENALTY=1.279252
PRIMER_RIGHT_30_PENALTY=1.201830
PRIMER_LEFT_30_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_30_SEQUENCE=TACTTGCCACAACAACGTCAA
PRIMER_LEFT_30=637,20
PRIMER_RIGHT_30=1288,21
PRIMER_LEFT_30_TM=58.721
PRIMER_RIGHT_30_TM=60.202
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=652
PRIMER_PAIR_31_PENALTY=2.481083
PRIMER_LEFT_31_PENALTY=1.279252
PRIMER_RIGHT_31_PENALTY=1.201830
PRIMER_LEFT_31_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_31_SEQUENCE=TTACTTGCCACAACAACGTCA
PRIMER_LEFT_31=637,20
PRIMER_RIGHT_31=1289,21
PRIMER_LEFT_31_TM=58.721
PRIMER_RIGHT_31_TM=60.202
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=653
PRIMER_PAIR_32_PENALTY=2.514534
PRIMER_LEFT_32_PENALTY=2.316034
PRIMER_RIGHT_32_PENALTY=0.198500
PRIMER_LEFT_32_SEQUENCE=GGAACTTGTGAAAATTGAGCC
PRIMER_RIGHT_32_SEQUENCE=ACTTGCCACAACAACGTCAA
PRIMER_LEFT_32=604,21
PRIMER_RIGHT_32=1287,20
PRIMER_LEFT_32_TM=58.684
PRIMER_RIGHT_32_TM=60.199
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=684
PRIMER_PAIR_33_PENALTY=2.516823
PRIMER_LEFT_33_PENALTY=2.460467
PRIMER_RIGHT_33_PENALTY=0.056356
PRIMER_LEFT_33_SEQUENCE=GAAAAAGTCTGCGTCTGTGAAA
PRIMER_RIGHT_33_SEQUENCE=GCCACAACAACGTCAACAAC
PRIMER_LEFT_33=727,22
PRIMER_RIGHT_33=1283,20
PRIMER_LEFT_33_TM=59.540
PRIMER_RIGHT_33_TM=60.056
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=557
PRIMER_PAIR_34_PENALTY=2.522328
PRIMER_LEFT_34_PENALTY=1.279252
PRIMER_RIGHT_34_PENALTY=1.243076
PRIMER_LEFT_34_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_34_SEQUENCE=TACTTGCCACAACAACGTCA
PRIMER_LEFT_34=638,20
PRIMER_RIGHT_34=1288,20
PRIMER_LEFT_34_TM=58.721
PRIMER_RIGHT_34_TM=58.757
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=651
PRIMER_PAIR_35_PENALTY=2.522328
PRIMER_LEFT_35_PENALTY=1.279252
PRIMER_RIGHT_35_PENALTY=1.243076
PRIMER_LEFT_35_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_35_SEQUENCE=TACTTGCCACAACAACGTCA
PRIMER_LEFT_35=637,20
PRIMER_RIGHT_35=1288,20
PRIMER_LEFT_35_TM=58.721
PRIMER_RIGHT_35_TM=58.757
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.6000
PRIMER_RIGHT_35_END_STABILITY=8.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=652
PRIMER_PAIR_36_PENALTY=2.563120
PRIMER_LEFT_36_PENALTY=2.460467
PRIMER_RIGHT_36_PENALTY=0.102654
PRIMER_LEFT_36_SEQUENCE=GAAAAAGTCTGCGTCTGTGAAA
PRIMER_RIGHT_36_SEQUENCE=CCACAACAACGTCAACAACC
PRIMER_LEFT_36=727,22
PRIMER_RIGHT_36=1282,20
PRIMER_LEFT_36_TM=59.540
PRIMER_RIGHT_36_TM=59.897
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=556
PRIMER_PAIR_37_PENALTY=2.576445
PRIMER_LEFT_37_PENALTY=0.265715
PRIMER_RIGHT_37_PENALTY=2.310730
PRIMER_LEFT_37_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_37_SEQUENCE=TGTACAAATGACGCAAACCTC
PRIMER_LEFT_37=636,20
PRIMER_RIGHT_37=1601,21
PRIMER_LEFT_37_TM=59.734
PRIMER_RIGHT_37_TM=58.689
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.9000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=966
PRIMER_PAIR_38_PENALTY=2.654258
PRIMER_LEFT_38_PENALTY=1.279252
PRIMER_RIGHT_38_PENALTY=1.375005
PRIMER_LEFT_38_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_38_SEQUENCE=TACAAATGACGCAAACCTCC
PRIMER_LEFT_38=637,20
PRIMER_RIGHT_38=1599,20
PRIMER_LEFT_38_TM=58.721
PRIMER_RIGHT_38_TM=58.625
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=963
PRIMER_PAIR_39_PENALTY=2.656028
PRIMER_LEFT_39_PENALTY=1.279252
PRIMER_RIGHT_39_PENALTY=1.376776
PRIMER_LEFT_39_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_39_SEQUENCE=TACAAATGACGCAAACCTCCT
PRIMER_LEFT_39=638,20
PRIMER_RIGHT_39=1599,21
PRIMER_LEFT_39_TM=58.721
PRIMER_RIGHT_39_TM=59.623
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=962
PRIMER_PAIR_40_PENALTY=2.656028
PRIMER_LEFT_40_PENALTY=1.279252
PRIMER_RIGHT_40_PENALTY=1.376776
PRIMER_LEFT_40_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_40_SEQUENCE=TACAAATGACGCAAACCTCCT
PRIMER_LEFT_40=637,20
PRIMER_RIGHT_40=1599,21
PRIMER_LEFT_40_TM=58.721
PRIMER_RIGHT_40_TM=59.623
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=963
PRIMER_PAIR_41_PENALTY=2.658967
PRIMER_LEFT_41_PENALTY=2.460467
PRIMER_RIGHT_41_PENALTY=0.198500
PRIMER_LEFT_41_SEQUENCE=GAAAAAGTCTGCGTCTGTGAAA
PRIMER_RIGHT_41_SEQUENCE=ACTTGCCACAACAACGTCAA
PRIMER_LEFT_41=727,22
PRIMER_RIGHT_41=1287,20
PRIMER_LEFT_41_TM=59.540
PRIMER_RIGHT_41_TM=60.199
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=561
PRIMER_PAIR_42_PENALTY=2.678311
PRIMER_LEFT_42_PENALTY=0.265715
PRIMER_RIGHT_42_PENALTY=2.412596
PRIMER_LEFT_42_SEQUENCE=GAACGAAGAACGGTACGGAG
PRIMER_RIGHT_42_SEQUENCE=GTACAAATGACGCAAACCTCCT
PRIMER_LEFT_42=636,20
PRIMER_RIGHT_42=1600,22
PRIMER_LEFT_42_TM=59.734
PRIMER_RIGHT_42_TM=60.413
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=965
PRIMER_PAIR_43_PENALTY=2.718496
PRIMER_LEFT_43_PENALTY=2.316034
PRIMER_RIGHT_43_PENALTY=0.402462
PRIMER_LEFT_43_SEQUENCE=GGAACTTGTGAAAATTGAGCC
PRIMER_RIGHT_43_SEQUENCE=ACAAATGACGCAAACCTCCT
PRIMER_LEFT_43=604,21
PRIMER_RIGHT_43=1598,20
PRIMER_LEFT_43_TM=58.684
PRIMER_RIGHT_43_TM=59.598
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=995
PRIMER_PAIR_44_PENALTY=2.744531
PRIMER_LEFT_44_PENALTY=1.542700
PRIMER_RIGHT_44_PENALTY=1.201830
PRIMER_LEFT_44_SEQUENCE=GAACGAAGAACGGTACGGAGT
PRIMER_RIGHT_44_SEQUENCE=TACTTGCCACAACAACGTCAA
PRIMER_LEFT_44=636,21
PRIMER_RIGHT_44=1288,21
PRIMER_LEFT_44_TM=60.543
PRIMER_RIGHT_44_TM=60.202
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=653
PRIMER_PAIR_45_PENALTY=2.744531
PRIMER_LEFT_45_PENALTY=1.542700
PRIMER_RIGHT_45_PENALTY=1.201830
PRIMER_LEFT_45_SEQUENCE=GAACGAAGAACGGTACGGAGT
PRIMER_RIGHT_45_SEQUENCE=TTACTTGCCACAACAACGTCA
PRIMER_LEFT_45=636,21
PRIMER_RIGHT_45=1289,21
PRIMER_LEFT_45_TM=60.543
PRIMER_RIGHT_45_TM=60.202
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=654
PRIMER_PAIR_46_PENALTY=2.785776
PRIMER_LEFT_46_PENALTY=1.542700
PRIMER_RIGHT_46_PENALTY=1.243076
PRIMER_LEFT_46_SEQUENCE=GAACGAAGAACGGTACGGAGT
PRIMER_RIGHT_46_SEQUENCE=TACTTGCCACAACAACGTCA
PRIMER_LEFT_46=636,21
PRIMER_RIGHT_46=1288,20
PRIMER_LEFT_46_TM=60.543
PRIMER_RIGHT_46_TM=58.757
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.6000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=653
PRIMER_PAIR_47_PENALTY=2.789547
PRIMER_LEFT_47_PENALTY=1.279252
PRIMER_RIGHT_47_PENALTY=1.510294
PRIMER_LEFT_47_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_47_SEQUENCE=GTACAAATGACGCAAACCTCC
PRIMER_LEFT_47=637,20
PRIMER_RIGHT_47=1600,21
PRIMER_LEFT_47_TM=58.721
PRIMER_RIGHT_47_TM=59.490
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=964
PRIMER_PAIR_48_PENALTY=2.877721
PRIMER_LEFT_48_PENALTY=1.279252
PRIMER_RIGHT_48_PENALTY=1.598468
PRIMER_LEFT_48_SEQUENCE=ACGAAGAACGGTACGGAGTT
PRIMER_RIGHT_48_SEQUENCE=CAAATGACGCAAACCTCCTG
PRIMER_LEFT_48=638,20
PRIMER_RIGHT_48=1597,20
PRIMER_LEFT_48_TM=58.721
PRIMER_RIGHT_48_TM=61.598
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=960
PRIMER_PAIR_49_PENALTY=2.877721
PRIMER_LEFT_49_PENALTY=1.279252
PRIMER_RIGHT_49_PENALTY=1.598468
PRIMER_LEFT_49_SEQUENCE=AACGAAGAACGGTACGGAGT
PRIMER_RIGHT_49_SEQUENCE=CAAATGACGCAAACCTCCTG
PRIMER_LEFT_49=637,20
PRIMER_RIGHT_49=1597,20
PRIMER_LEFT_49_TM=58.721
PRIMER_RIGHT_49_TM=61.598
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=961
=
SEQUENCE_ID=MAGIC.338_2_8566433_8566496_ct-1_wil-2_4406_8697194_TRUE
SEQUENCE_TARGET=950,163
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNTTTTTTNATANAAAAAATAGNANNTGTNCANTAATAGAGAAATCAATCATCTATAATAAGCAAATGANGTCAGACTACAGAAGACGATAGAAGAATCATCTTTCTCCACCGTTGTTTTCTAGAGAACTACTTGCGGACCACTTATTAATTTTAGGGATATGATATTGANAGCGACTAACCAAATCGATATGAACTAATATANAATGAAAATAAACAAAACTGTATATATATTATTTAAACTGTGAAAGTTCAATTTCATTCACGTCACTGAATTCAAAATATCAAACGATATGAATTTTATCACCTTTTCGTTTTCAGCAAAATTGGATTTAGAAATGTAATAGTATTTCCATATAACAAAACGTTTTTATTAAACATTAACGTTAATGGCACANACCGAAGAATATCCTAAAAAATTGCGCCACCTGGTTCAAGTCGCTATGGATGGGATGGTGTGTCGTCTGCTCATCAACGTACATTTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_14455323_14455339_tsu-0_no-0_16390_14222_TRUE
SEQUENCE_TARGET=950,116
SEQUENCE_TEMPLATE=AACTNCAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTACNAAAAAAANTNNATATTCTTACGNAAAAATTNCNNNCATTNTTTTCNTCAGAANATTGTGACNATTTNCATACAAAATTNTGACGTATTTATTTGGNCATAAAAACGTTACTATTTCGTTATAATATTATGACTTAATTGAANGTAACTAATGTTTGTCACTAATTATGTCAAATTTGTTACAAATTATATAGTCATAAATATNANNNNNNNNNNGTNACCAAATATGGTAACTAAATTATACGATAATTGTGACTGAATTTATGTCATGTATAGTTACGAGAATTTTGTAACAACGATGACACAATTTGTCACTNTTTTAGTGACTTTTGCGTTTGGTCATAGATTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17196171_17196279_bur-0_po-0_2020_159649_TRUE
SEQUENCE_TARGET=950,208
SEQUENCE_TEMPLATE=ANGTTTGTTGTCGGGAATGNTGGATNTAGNGGTTNAGACACAGGACNAAACTGNTGNAACATACNANTNTAATGNAATNATNNNAAGNNAATGNNNGNAACAAACCTAATNAAAGCCATCTGAGGATTCATCTTNCCAGTNGCAACTTTGACAAAATCATCATCCTTGAAGGANAACGTAGCATCCACTTTCCCTCCTTCATATTTCCCTAATTTCCAAAGATCAAAANNATNAACGGAAAGTCAATAACTNAAAGCAANNNNNNNNNNNNNNNNNATTTCAATTCCCAATTGCCATAACCAANAAGAAAAACNTAACCTTTGATGCAAAANCAAATNTCANGATTCTCTCTTNTCTAAATNCNCAANTACTCTTCACNTTTGTCTCCAATCNAATTTNCAGATTCCCAATTGCAANAATCNCAAAATNAAAACTTTCAGATTTTACCATNANCAAAANANAAAANNCTAACCTTTGGTGACTTCGCCTTTCTTCAGATCAACGATGTAAGTAACCTCTTCAAACCCTAATTTCTGAAATTCCAAATCTNTCACAATCAAANANAAAACAAAACAGAGTAANAANNNAAATGANNCTATAAAGAAAGGACCAACCTTTGGAGCAATGTTGATCTGATAAACAAGACCGATCTTCTCTGTGACCTCTTTCCCGGCGTCGGTGGAAAGATGTTCCTTCATCATGTCCATGATNGCATCGGATTTGAGTTGGGTATTCGCCNTNTNTTTNTTGNGCAGTGNAGTGNAATNTCGGAGAAGAAAGGTGAAAGATTGGATTTTTAAGGATGGAGTTAGGTAATAGAGACAAAGGAGGTGACGTGATCGATAAGTATGGAAAGATTGAGATTTTTGTTAGAGNCTGGCACGCTTCCCTATTTGCTTATTCTTACCTCATGATTACTGTTTTTCTATNTGTTTCTATTTTGGGGAGATTTAGCAGANTAATTATGTTGACCAGAANNNAAANAACAGNAANNNNNNNNNTCATCTTNNGCAAATTGNGATTNTGTCCTNATATTTGTTNNTNNCCCTAATTATAAAACCAANNTTTNCAATGAAGANTGAATAACNATGTATNNNTTGTATTTAAANAGCAAATNNNNNNNNNNNNGNTATTTGGTGTGATTTGCNNTGACATCAATTNGAAAGCTTTGGGACAGNNCTNAAAANGACTAGTNAAAAAAAANNNNNNNNAAAANATTANTTTCAGGAGGATTGTTGTTCTGTTTNTTCGTTCTCGNAGGTTTNTGTTTNNTTTCATGCTTTTGGGCTTTNGGCCGTATGNGCNTNTTNCCNTGTANTGTTGTATTTTNANTGGAAANGGTGTTTNCGTTCANNGATTNATTATGTGAAACCGNTTATANTTTNATNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCAGCTCTATTNNAATTTATAAAACNNCTAATTAAAGACCTAATTANAACANTTCNNAGACGTTAAACTAANATAATTNANATCANANTTTCACATTTCATTNCTTNTAAATAAGCANANNNNNNNNNNNNNNNNNNNANNAAAAACANNNNNAACANGCATTAANCATATATTTNGTNATACTAANNTTTTTTATTCAATTTNTNGTTATGTNTATNNAAAATGTGCTTTCACTTCTTNCNGTTNTATGCATNATNGTTTNTGTAAATGCTCAANTGCCANANATTCNTNNTCCGTTTCCTTTTNNATTTNCGTTCCAACCGATTCNTGGTATGCNAGGATTACNTGANATAACAAAATGTTTGTCATCCGTGATGGATATTCCCNGATGCATTACAGAAATTTCTCAATCTATTTTCATTGGAAAATTCGGTAATTTAGGTNCNNCNTGTTGCAAANCATTTTTGGATGCNNNAGCCAATTNTATGCCNAAAATTCNATNCATTCCGCNTTTNNCTCNNATGCTAAANNANCAATGTTCAANNATTNNTGGTGCAACTCCTNCNACACCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.558941
PRIMER_LEFT_0_PENALTY=1.611571
PRIMER_RIGHT_0_PENALTY=0.947369
PRIMER_LEFT_0_SEQUENCE=GCACGCTTCCCTATTTGCTT
PRIMER_RIGHT_0_SEQUENCE=TCCATCACGGATGACAAACA
PRIMER_LEFT_0=879,20
PRIMER_RIGHT_0=1793,20
PRIMER_LEFT_0_TM=61.612
PRIMER_RIGHT_0_TM=60.947
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=915
PRIMER_PAIR_1_PENALTY=2.801719
PRIMER_LEFT_1_PENALTY=0.016312
PRIMER_RIGHT_1_PENALTY=2.785407
PRIMER_LEFT_1_SEQUENCE=ATTCCCAATTGCCATAACCA
PRIMER_RIGHT_1_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_1=282,20
PRIMER_RIGHT_1=1242,22
PRIMER_LEFT_1_TM=60.016
PRIMER_RIGHT_1_TM=59.215
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=961
PRIMER_PAIR_2_PENALTY=2.932703
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=2.785407
PRIMER_LEFT_2_SEQUENCE=TTGGTGACTTCGCCTTTCTT
PRIMER_RIGHT_2_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_2=474,20
PRIMER_RIGHT_2=1242,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=59.215
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=769
PRIMER_PAIR_3_PENALTY=2.932703
PRIMER_LEFT_3_PENALTY=0.147295
PRIMER_RIGHT_3_PENALTY=2.785407
PRIMER_LEFT_3_SEQUENCE=TTTGGTGACTTCGCCTTTCT
PRIMER_RIGHT_3_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_3=473,20
PRIMER_RIGHT_3=1242,22
PRIMER_LEFT_3_TM=59.853
PRIMER_RIGHT_3_TM=59.215
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=770
PRIMER_PAIR_4_PENALTY=3.160497
PRIMER_LEFT_4_PENALTY=0.375090
PRIMER_RIGHT_4_PENALTY=2.785407
PRIMER_LEFT_4_SEQUENCE=GGTGACTTCGCCTTTCTTCA
PRIMER_RIGHT_4_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_4=476,20
PRIMER_RIGHT_4=1242,22
PRIMER_LEFT_4_TM=60.375
PRIMER_RIGHT_4_TM=59.215
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=767
PRIMER_PAIR_5_PENALTY=3.160497
PRIMER_LEFT_5_PENALTY=0.375090
PRIMER_RIGHT_5_PENALTY=2.785407
PRIMER_LEFT_5_SEQUENCE=TGGTGACTTCGCCTTTCTTC
PRIMER_RIGHT_5_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_5=475,20
PRIMER_RIGHT_5=1242,22
PRIMER_LEFT_5_TM=60.375
PRIMER_RIGHT_5_TM=59.215
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=768
PRIMER_PAIR_6_PENALTY=3.232944
PRIMER_LEFT_6_PENALTY=0.016312
PRIMER_RIGHT_6_PENALTY=3.216632
PRIMER_LEFT_6_SEQUENCE=ATTCCCAATTGCCATAACCA
PRIMER_RIGHT_6_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_6=282,20
PRIMER_RIGHT_6=1242,21
PRIMER_LEFT_6_TM=60.016
PRIMER_RIGHT_6_TM=57.783
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=961
PRIMER_PAIR_7_PENALTY=3.282703
PRIMER_LEFT_7_PENALTY=2.335333
PRIMER_RIGHT_7_PENALTY=0.947369
PRIMER_LEFT_7_SEQUENCE=CACGCTTCCCTATTTGCTTATT
PRIMER_RIGHT_7_SEQUENCE=TCCATCACGGATGACAAACA
PRIMER_LEFT_7=880,22
PRIMER_RIGHT_7=1793,20
PRIMER_LEFT_7_TM=59.665
PRIMER_RIGHT_7_TM=60.947
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.2000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=914
PRIMER_PAIR_8_PENALTY=3.320988
PRIMER_LEFT_8_PENALTY=0.016312
PRIMER_RIGHT_8_PENALTY=3.304676
PRIMER_LEFT_8_SEQUENCE=ATTCCCAATTGCCATAACCA
PRIMER_RIGHT_8_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_8=282,20
PRIMER_RIGHT_8=1243,22
PRIMER_LEFT_8_TM=60.016
PRIMER_RIGHT_8_TM=58.695
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=962
PRIMER_PAIR_9_PENALTY=3.363928
PRIMER_LEFT_9_PENALTY=0.147295
PRIMER_RIGHT_9_PENALTY=3.216632
PRIMER_LEFT_9_SEQUENCE=TTGGTGACTTCGCCTTTCTT
PRIMER_RIGHT_9_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_9=474,20
PRIMER_RIGHT_9=1242,21
PRIMER_LEFT_9_TM=59.853
PRIMER_RIGHT_9_TM=57.783
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=769
PRIMER_PAIR_10_PENALTY=3.363928
PRIMER_LEFT_10_PENALTY=0.147295
PRIMER_RIGHT_10_PENALTY=3.216632
PRIMER_LEFT_10_SEQUENCE=TTTGGTGACTTCGCCTTTCT
PRIMER_RIGHT_10_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_10=473,20
PRIMER_RIGHT_10=1242,21
PRIMER_LEFT_10_TM=59.853
PRIMER_RIGHT_10_TM=57.783
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=770
PRIMER_PAIR_11_PENALTY=3.363928
PRIMER_LEFT_11_PENALTY=0.147295
PRIMER_RIGHT_11_PENALTY=3.216632
PRIMER_LEFT_11_SEQUENCE=CTTTGGTGACTTCGCCTTTC
PRIMER_RIGHT_11_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_11=472,20
PRIMER_RIGHT_11=1242,21
PRIMER_LEFT_11_TM=59.853
PRIMER_RIGHT_11_TM=57.783
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=771
PRIMER_PAIR_12_PENALTY=3.451971
PRIMER_LEFT_12_PENALTY=0.147295
PRIMER_RIGHT_12_PENALTY=3.304676
PRIMER_LEFT_12_SEQUENCE=TTGGTGACTTCGCCTTTCTT
PRIMER_RIGHT_12_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_12=474,20
PRIMER_RIGHT_12=1243,22
PRIMER_LEFT_12_TM=59.853
PRIMER_RIGHT_12_TM=58.695
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=770
PRIMER_PAIR_13_PENALTY=3.451971
PRIMER_LEFT_13_PENALTY=0.147295
PRIMER_RIGHT_13_PENALTY=3.304676
PRIMER_LEFT_13_SEQUENCE=TTTGGTGACTTCGCCTTTCT
PRIMER_RIGHT_13_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_13=473,20
PRIMER_RIGHT_13=1243,22
PRIMER_LEFT_13_TM=59.853
PRIMER_RIGHT_13_TM=58.695
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=771
PRIMER_PAIR_14_PENALTY=3.451971
PRIMER_LEFT_14_PENALTY=0.147295
PRIMER_RIGHT_14_PENALTY=3.304676
PRIMER_LEFT_14_SEQUENCE=CTTTGGTGACTTCGCCTTTC
PRIMER_RIGHT_14_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_14=472,20
PRIMER_RIGHT_14=1243,22
PRIMER_LEFT_14_TM=59.853
PRIMER_RIGHT_14_TM=58.695
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=772
PRIMER_PAIR_15_PENALTY=3.591722
PRIMER_LEFT_15_PENALTY=0.375090
PRIMER_RIGHT_15_PENALTY=3.216632
PRIMER_LEFT_15_SEQUENCE=TGGTGACTTCGCCTTTCTTC
PRIMER_RIGHT_15_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_15=475,20
PRIMER_RIGHT_15=1242,21
PRIMER_LEFT_15_TM=60.375
PRIMER_RIGHT_15_TM=57.783
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=768
PRIMER_PAIR_16_PENALTY=3.679766
PRIMER_LEFT_16_PENALTY=0.375090
PRIMER_RIGHT_16_PENALTY=3.304676
PRIMER_LEFT_16_SEQUENCE=TGGTGACTTCGCCTTTCTTC
PRIMER_RIGHT_16_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_16=475,20
PRIMER_RIGHT_16=1243,22
PRIMER_LEFT_16_TM=60.375
PRIMER_RIGHT_16_TM=58.695
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=769
PRIMER_PAIR_17_PENALTY=3.789722
PRIMER_LEFT_17_PENALTY=0.016312
PRIMER_RIGHT_17_PENALTY=3.773411
PRIMER_LEFT_17_SEQUENCE=ATTCCCAATTGCCATAACCA
PRIMER_RIGHT_17_SEQUENCE=AAACAGAACAACAATCCTCCTG
PRIMER_LEFT_17=282,20
PRIMER_RIGHT_17=1245,22
PRIMER_LEFT_17_TM=60.016
PRIMER_RIGHT_17_TM=58.227
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.909
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=964
PRIMER_PAIR_18_PENALTY=3.832106
PRIMER_LEFT_18_PENALTY=1.046699
PRIMER_RIGHT_18_PENALTY=2.785407
PRIMER_LEFT_18_SEQUENCE=ACCTTTGGTGACTTCGCCTT
PRIMER_RIGHT_18_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_18=470,20
PRIMER_RIGHT_18=1242,22
PRIMER_LEFT_18_TM=61.047
PRIMER_RIGHT_18_TM=59.215
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=773
PRIMER_PAIR_19_PENALTY=3.840554
PRIMER_LEFT_19_PENALTY=1.055146
PRIMER_RIGHT_19_PENALTY=2.785407
PRIMER_LEFT_19_SEQUENCE=TTCCCAATTGCCATAACCAA
PRIMER_RIGHT_19_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_19=283,20
PRIMER_RIGHT_19=1242,22
PRIMER_LEFT_19_TM=61.055
PRIMER_RIGHT_19_TM=59.215
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=960
PRIMER_PAIR_20_PENALTY=3.920706
PRIMER_LEFT_20_PENALTY=0.147295
PRIMER_RIGHT_20_PENALTY=3.773411
PRIMER_LEFT_20_SEQUENCE=TTGGTGACTTCGCCTTTCTT
PRIMER_RIGHT_20_SEQUENCE=AAACAGAACAACAATCCTCCTG
PRIMER_LEFT_20=474,20
PRIMER_RIGHT_20=1245,22
PRIMER_LEFT_20_TM=59.853
PRIMER_RIGHT_20_TM=58.227
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=772
PRIMER_PAIR_21_PENALTY=3.920706
PRIMER_LEFT_21_PENALTY=0.147295
PRIMER_RIGHT_21_PENALTY=3.773411
PRIMER_LEFT_21_SEQUENCE=TTTGGTGACTTCGCCTTTCT
PRIMER_RIGHT_21_SEQUENCE=AAACAGAACAACAATCCTCCTG
PRIMER_LEFT_21=473,20
PRIMER_RIGHT_21=1245,22
PRIMER_LEFT_21_TM=59.853
PRIMER_RIGHT_21_TM=58.227
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=773
PRIMER_PAIR_22_PENALTY=3.920706
PRIMER_LEFT_22_PENALTY=0.147295
PRIMER_RIGHT_22_PENALTY=3.773411
PRIMER_LEFT_22_SEQUENCE=CTTTGGTGACTTCGCCTTTC
PRIMER_RIGHT_22_SEQUENCE=AAACAGAACAACAATCCTCCTG
PRIMER_LEFT_22=472,20
PRIMER_RIGHT_22=1245,22
PRIMER_LEFT_22_TM=59.853
PRIMER_RIGHT_22_TM=58.227
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=774
PRIMER_PAIR_23_PENALTY=3.980425
PRIMER_LEFT_23_PENALTY=3.033056
PRIMER_RIGHT_23_PENALTY=0.947369
PRIMER_LEFT_23_SEQUENCE=CGCTTCCCTATTTGCTTATTCT
PRIMER_RIGHT_23_SEQUENCE=TCCATCACGGATGACAAACA
PRIMER_LEFT_23=882,22
PRIMER_RIGHT_23=1793,20
PRIMER_LEFT_23_TM=58.967
PRIMER_RIGHT_23_TM=60.947
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=912
PRIMER_PAIR_24_PENALTY=4.148500
PRIMER_LEFT_24_PENALTY=0.375090
PRIMER_RIGHT_24_PENALTY=3.773411
PRIMER_LEFT_24_SEQUENCE=GGTGACTTCGCCTTTCTTCA
PRIMER_RIGHT_24_SEQUENCE=AAACAGAACAACAATCCTCCTG
PRIMER_LEFT_24=476,20
PRIMER_RIGHT_24=1245,22
PRIMER_LEFT_24_TM=60.375
PRIMER_RIGHT_24_TM=58.227
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=770
PRIMER_PAIR_25_PENALTY=4.148500
PRIMER_LEFT_25_PENALTY=0.375090
PRIMER_RIGHT_25_PENALTY=3.773411
PRIMER_LEFT_25_SEQUENCE=TGGTGACTTCGCCTTTCTTC
PRIMER_RIGHT_25_SEQUENCE=AAACAGAACAACAATCCTCCTG
PRIMER_LEFT_25=475,20
PRIMER_RIGHT_25=1245,22
PRIMER_LEFT_25_TM=60.375
PRIMER_RIGHT_25_TM=58.227
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=771
PRIMER_PAIR_26_PENALTY=4.161352
PRIMER_LEFT_26_PENALTY=1.375944
PRIMER_RIGHT_26_PENALTY=2.785407
PRIMER_LEFT_26_SEQUENCE=AATTCCCAATTGCCATAACC
PRIMER_RIGHT_26_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_26=281,20
PRIMER_RIGHT_26=1242,22
PRIMER_LEFT_26_TM=58.624
PRIMER_RIGHT_26_TM=59.215
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.2000
PRIMER_RIGHT_26_END_STABILITY=7.3000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=962
PRIMER_PAIR_27_PENALTY=4.167123
PRIMER_LEFT_27_PENALTY=1.381716
PRIMER_RIGHT_27_PENALTY=2.785407
PRIMER_LEFT_27_SEQUENCE=GAAAGGACCAACCTTTGGAG
PRIMER_RIGHT_27_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_27=602,20
PRIMER_RIGHT_27=1242,22
PRIMER_LEFT_27_TM=58.618
PRIMER_RIGHT_27_TM=59.215
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=641
PRIMER_PAIR_28_PENALTY=4.183427
PRIMER_LEFT_28_PENALTY=1.398020
PRIMER_RIGHT_28_PENALTY=2.785407
PRIMER_LEFT_28_SEQUENCE=AGAAAGGACCAACCTTTGGAG
PRIMER_RIGHT_28_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_28=601,21
PRIMER_RIGHT_28=1242,22
PRIMER_LEFT_28_TM=59.602
PRIMER_RIGHT_28_TM=59.215
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=642
PRIMER_PAIR_29_PENALTY=4.263331
PRIMER_LEFT_29_PENALTY=1.046699
PRIMER_RIGHT_29_PENALTY=3.216632
PRIMER_LEFT_29_SEQUENCE=ACCTTTGGTGACTTCGCCTT
PRIMER_RIGHT_29_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_29=470,20
PRIMER_RIGHT_29=1242,21
PRIMER_LEFT_29_TM=61.047
PRIMER_RIGHT_29_TM=57.783
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=773
PRIMER_PAIR_30_PENALTY=4.263796
PRIMER_LEFT_30_PENALTY=0.016312
PRIMER_RIGHT_30_PENALTY=4.247485
PRIMER_LEFT_30_SEQUENCE=ATTCCCAATTGCCATAACCA
PRIMER_RIGHT_30_SEQUENCE=AACAGAACAACAATCCTCCTG
PRIMER_LEFT_30=282,20
PRIMER_RIGHT_30=1244,21
PRIMER_LEFT_30_TM=60.016
PRIMER_RIGHT_30_TM=56.753
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=963
PRIMER_PAIR_31_PENALTY=4.271779
PRIMER_LEFT_31_PENALTY=1.055146
PRIMER_RIGHT_31_PENALTY=3.216632
PRIMER_LEFT_31_SEQUENCE=TTCCCAATTGCCATAACCAA
PRIMER_RIGHT_31_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_31=283,20
PRIMER_RIGHT_31=1242,21
PRIMER_LEFT_31_TM=61.055
PRIMER_RIGHT_31_TM=57.783
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=960
PRIMER_PAIR_32_PENALTY=4.311382
PRIMER_LEFT_32_PENALTY=1.525975
PRIMER_RIGHT_32_PENALTY=2.785407
PRIMER_LEFT_32_SEQUENCE=CCTTTGGTGACTTCGCCTTT
PRIMER_RIGHT_32_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_32=471,20
PRIMER_RIGHT_32=1242,22
PRIMER_LEFT_32_TM=61.526
PRIMER_RIGHT_32_TM=59.215
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=772
PRIMER_PAIR_33_PENALTY=4.342176
PRIMER_LEFT_33_PENALTY=1.556768
PRIMER_RIGHT_33_PENALTY=2.785407
PRIMER_LEFT_33_SEQUENCE=TTCGCCTTTCTTCAGATCAAC
PRIMER_RIGHT_33_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_33=482,21
PRIMER_RIGHT_33=1242,22
PRIMER_LEFT_33_TM=59.443
PRIMER_RIGHT_33_TM=59.215
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=40.909
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=761
PRIMER_PAIR_34_PENALTY=4.351375
PRIMER_LEFT_34_PENALTY=1.046699
PRIMER_RIGHT_34_PENALTY=3.304676
PRIMER_LEFT_34_SEQUENCE=ACCTTTGGTGACTTCGCCTT
PRIMER_RIGHT_34_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_34=470,20
PRIMER_RIGHT_34=1243,22
PRIMER_LEFT_34_TM=61.047
PRIMER_RIGHT_34_TM=58.695
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=774
PRIMER_PAIR_35_PENALTY=4.359822
PRIMER_LEFT_35_PENALTY=1.055146
PRIMER_RIGHT_35_PENALTY=3.304676
PRIMER_LEFT_35_SEQUENCE=TTCCCAATTGCCATAACCAA
PRIMER_RIGHT_35_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_35=283,20
PRIMER_RIGHT_35=1243,22
PRIMER_LEFT_35_TM=61.055
PRIMER_RIGHT_35_TM=58.695
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=961
PRIMER_PAIR_36_PENALTY=4.394780
PRIMER_LEFT_36_PENALTY=0.147295
PRIMER_RIGHT_36_PENALTY=4.247485
PRIMER_LEFT_36_SEQUENCE=TTGGTGACTTCGCCTTTCTT
PRIMER_RIGHT_36_SEQUENCE=AACAGAACAACAATCCTCCTG
PRIMER_LEFT_36=474,20
PRIMER_RIGHT_36=1244,21
PRIMER_LEFT_36_TM=59.853
PRIMER_RIGHT_36_TM=56.753
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=771
PRIMER_PAIR_37_PENALTY=4.394780
PRIMER_LEFT_37_PENALTY=0.147295
PRIMER_RIGHT_37_PENALTY=4.247485
PRIMER_LEFT_37_SEQUENCE=TTTGGTGACTTCGCCTTTCT
PRIMER_RIGHT_37_SEQUENCE=AACAGAACAACAATCCTCCTG
PRIMER_LEFT_37=473,20
PRIMER_RIGHT_37=1244,21
PRIMER_LEFT_37_TM=59.853
PRIMER_RIGHT_37_TM=56.753
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=772
PRIMER_PAIR_38_PENALTY=4.394780
PRIMER_LEFT_38_PENALTY=0.147295
PRIMER_RIGHT_38_PENALTY=4.247485
PRIMER_LEFT_38_SEQUENCE=CTTTGGTGACTTCGCCTTTC
PRIMER_RIGHT_38_SEQUENCE=AACAGAACAACAATCCTCCTG
PRIMER_LEFT_38=472,20
PRIMER_RIGHT_38=1244,21
PRIMER_LEFT_38_TM=59.853
PRIMER_RIGHT_38_TM=56.753
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=773
PRIMER_PAIR_39_PENALTY=4.563052
PRIMER_LEFT_39_PENALTY=1.777645
PRIMER_RIGHT_39_PENALTY=2.785407
PRIMER_LEFT_39_SEQUENCE=CTTTGGTGACTTCGCCTTTCT
PRIMER_RIGHT_39_SEQUENCE=CAGAACAACAATCCTCCTGAAA
PRIMER_LEFT_39=472,21
PRIMER_RIGHT_39=1242,22
PRIMER_LEFT_39_TM=60.778
PRIMER_RIGHT_39_TM=59.215
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=771
PRIMER_PAIR_40_PENALTY=4.592577
PRIMER_LEFT_40_PENALTY=1.375944
PRIMER_RIGHT_40_PENALTY=3.216632
PRIMER_LEFT_40_SEQUENCE=AATTCCCAATTGCCATAACC
PRIMER_RIGHT_40_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_40=281,20
PRIMER_RIGHT_40=1242,21
PRIMER_LEFT_40_TM=58.624
PRIMER_RIGHT_40_TM=57.783
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.2000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=962
PRIMER_PAIR_41_PENALTY=4.598348
PRIMER_LEFT_41_PENALTY=1.381716
PRIMER_RIGHT_41_PENALTY=3.216632
PRIMER_LEFT_41_SEQUENCE=GAAAGGACCAACCTTTGGAG
PRIMER_RIGHT_41_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_41=602,20
PRIMER_RIGHT_41=1242,21
PRIMER_LEFT_41_TM=58.618
PRIMER_RIGHT_41_TM=57.783
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=641
PRIMER_PAIR_42_PENALTY=4.614652
PRIMER_LEFT_42_PENALTY=1.398020
PRIMER_RIGHT_42_PENALTY=3.216632
PRIMER_LEFT_42_SEQUENCE=AGAAAGGACCAACCTTTGGAG
PRIMER_RIGHT_42_SEQUENCE=CAGAACAACAATCCTCCTGAA
PRIMER_LEFT_42=601,21
PRIMER_RIGHT_42=1242,21
PRIMER_LEFT_42_TM=59.602
PRIMER_RIGHT_42_TM=57.783
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=642
PRIMER_PAIR_43_PENALTY=4.622575
PRIMER_LEFT_43_PENALTY=0.375090
PRIMER_RIGHT_43_PENALTY=4.247485
PRIMER_LEFT_43_SEQUENCE=GGTGACTTCGCCTTTCTTCA
PRIMER_RIGHT_43_SEQUENCE=AACAGAACAACAATCCTCCTG
PRIMER_LEFT_43=476,20
PRIMER_RIGHT_43=1244,21
PRIMER_LEFT_43_TM=60.375
PRIMER_RIGHT_43_TM=56.753
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=769
PRIMER_PAIR_44_PENALTY=4.622575
PRIMER_LEFT_44_PENALTY=0.375090
PRIMER_RIGHT_44_PENALTY=4.247485
PRIMER_LEFT_44_SEQUENCE=TGGTGACTTCGCCTTTCTTC
PRIMER_RIGHT_44_SEQUENCE=AACAGAACAACAATCCTCCTG
PRIMER_LEFT_44=475,20
PRIMER_RIGHT_44=1244,21
PRIMER_LEFT_44_TM=60.375
PRIMER_RIGHT_44_TM=56.753
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=770
PRIMER_PAIR_45_PENALTY=4.645873
PRIMER_LEFT_45_PENALTY=3.698504
PRIMER_RIGHT_45_PENALTY=0.947369
PRIMER_LEFT_45_SEQUENCE=GGCACGCTTCCCTATTTGCT
PRIMER_RIGHT_45_SEQUENCE=TCCATCACGGATGACAAACA
PRIMER_LEFT_45=878,20
PRIMER_RIGHT_45=1793,20
PRIMER_LEFT_45_TM=63.699
PRIMER_RIGHT_45_TM=60.947
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=916
PRIMER_PAIR_46_PENALTY=4.667411
PRIMER_LEFT_46_PENALTY=3.720041
PRIMER_RIGHT_46_PENALTY=0.947369
PRIMER_LEFT_46_SEQUENCE=CGCTTCCCTATTTGCTTATT
PRIMER_RIGHT_46_SEQUENCE=TCCATCACGGATGACAAACA
PRIMER_LEFT_46=882,20
PRIMER_RIGHT_46=1793,20
PRIMER_LEFT_46_TM=56.280
PRIMER_RIGHT_46_TM=60.947
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.2000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=912
PRIMER_PAIR_47_PENALTY=4.680620
PRIMER_LEFT_47_PENALTY=1.375944
PRIMER_RIGHT_47_PENALTY=3.304676
PRIMER_LEFT_47_SEQUENCE=AATTCCCAATTGCCATAACC
PRIMER_RIGHT_47_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_47=281,20
PRIMER_RIGHT_47=1243,22
PRIMER_LEFT_47_TM=58.624
PRIMER_RIGHT_47_TM=58.695
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.2000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=963
PRIMER_PAIR_48_PENALTY=4.686392
PRIMER_LEFT_48_PENALTY=1.381716
PRIMER_RIGHT_48_PENALTY=3.304676
PRIMER_LEFT_48_SEQUENCE=GAAAGGACCAACCTTTGGAG
PRIMER_RIGHT_48_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_48=602,20
PRIMER_RIGHT_48=1243,22
PRIMER_LEFT_48_TM=58.618
PRIMER_RIGHT_48_TM=58.695
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=642
PRIMER_PAIR_49_PENALTY=4.702696
PRIMER_LEFT_49_PENALTY=1.398020
PRIMER_RIGHT_49_PENALTY=3.304676
PRIMER_LEFT_49_SEQUENCE=AGAAAGGACCAACCTTTGGAG
PRIMER_RIGHT_49_SEQUENCE=ACAGAACAACAATCCTCCTGAA
PRIMER_LEFT_49=601,21
PRIMER_RIGHT_49=1243,22
PRIMER_LEFT_49_TM=59.602
PRIMER_RIGHT_49_TM=58.695
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=643
=
SEQUENCE_ID=MAGIC.433_1_11300978_11301061_hi-0_oy-0_403747_23752_TRUE
SEQUENCE_TARGET=950,183
SEQUENCE_TEMPLATE=AGACAAAGAAAAAGAAACCTGTAGACAGATGAAATCAGCTTNNAAACTCNTTATATTATCAAGAATCTCCTTTGAACGGTTATCCCACCTATGNNAAAAANATTAAAAGNATANGCTTGATNTTTCTTTTGATAAACCATGAATNAACCAGATTGAACCGGAGTGGGCAAGTGGCNCGAGATGGGTTAGTCTGNNATGGTCCATCTNAATTTATCGGAATNNAATCCTTAGNNAGAGGNTTNTTTTATTTAGNTAGTGNNGGAATGAACATTTGGAATTTCTAATCATGAACCCCNNAANAAAATCATAAANAAAANNTTTGAAGANTNTACTTGAANAGTNTTCATCAATAANGAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGTATAAATTCAAGTTTNNATCAAACANTGTCNNNAATNGATATTNNNGATCTAANGAATCNTTAAGTTTGGNTNNNNNNNNNNNNNNNNNNNNNNTNTTTTCTTGGACTCACTTGAGAATGGGATATTNGTNTCTCAAAGCTCATACGCCAAGAGTCTNGTGACAAGGTTCAAAGTACATNCCAAGNTATATNANTTTCCAAAACACCCANGATCATTACGGTANAACCGTTNNNNNNNNNNNNNNNNNNNNNGATGAAGANGATCNGAAACTTGANGAGAAATTGTTTNAGACANTAANCCACAAACCAANAAGCTGCCCCACNNAAATAATGCAATGANACNNNNNNNNNNNNNATNNACACACNNNNNAATAATAAATATTAGAATAATNCANGGGGGNAAAGACATTTCNCCGAAGACTANGAAATGATCCAAAACTCGAAGACCCTTAANNNACTACTAAGTCGTNGATNGCTTCAACNNNTNTTNTCTTATCTCTCNNATATTTTANTCNTATTCTCTNCNATATATCTTNNCNTCTCTATTCTANATAAATGCNTAAACCTGAGTCACNCTGATAAGTAAATTAAGNAAGNAATATTAATTAAGATGNCATCTTCTNTAGTNTCCTGCAATATAGCTTTTNCNTNTNTCGNTNACANTCGATAANTCATTTTANAGCATNTGCAANGGNTAGAGNNNNTNNNNNNNNTTTATTTANTGTACAATGTGCAATGGNTAAAACTTANANAAATNATAANNNTNANTTNNNNNAANATNNANAANNNNNNNNNNNGAATCGNNNACNGNAACTANNCNAAATTNTATCANATTCGTTCTNGTTCNTTTTTTTCTTTNAAAATAACTCNTTTNNANATNTTCTAAGTTTCTGGATATTTTNGTTAACCGTTTGGTTNTNGGNNNNNTTTTATTTTATTTAAAACTTNAATCAATAACTCAANTAAATTATATGCATTAAAATAATATATTCGATAGACTTATAAAATCTCNAAATNAGTTNNNNNNNNNNNNNNNATCGANACACGGAAGGGCTATGTTGTAGGAGNTTTTTCAGATTGTGGNTNGACNTGTATTTATTTGANATNGAGNTTTNTNNTGNNAAAAAAAAATTANATANNGGNNTNNNTNTGCACANANTCCTTATGTTTTCAAACGGAGTTATCTAGTGCCACNTCATCTATACACGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.956055
PRIMER_LEFT_0_PENALTY=0.433612
PRIMER_RIGHT_0_PENALTY=0.522443
PRIMER_LEFT_0_SEQUENCE=TCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_0_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_0=537,20
PRIMER_RIGHT_0=1465,20
PRIMER_LEFT_0_TM=59.566
PRIMER_RIGHT_0_TM=59.478
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=929
PRIMER_PAIR_1_PENALTY=0.956055
PRIMER_LEFT_1_PENALTY=0.433612
PRIMER_RIGHT_1_PENALTY=0.522443
PRIMER_LEFT_1_SEQUENCE=TCTCAAAGCTCATACGCCAA
PRIMER_RIGHT_1_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_1=535,20
PRIMER_RIGHT_1=1465,20
PRIMER_LEFT_1_TM=59.566
PRIMER_RIGHT_1_TM=59.478
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=931
PRIMER_PAIR_2_PENALTY=1.618335
PRIMER_LEFT_2_PENALTY=1.095892
PRIMER_RIGHT_2_PENALTY=0.522443
PRIMER_LEFT_2_SEQUENCE=TTGGACTCACTTGAGAATGGG
PRIMER_RIGHT_2_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_2=505,21
PRIMER_RIGHT_2=1465,20
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.478
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=961
PRIMER_PAIR_3_PENALTY=1.784214
PRIMER_LEFT_3_PENALTY=0.433612
PRIMER_RIGHT_3_PENALTY=1.350602
PRIMER_LEFT_3_SEQUENCE=TCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_3_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_3=537,20
PRIMER_RIGHT_3=1466,20
PRIMER_LEFT_3_TM=59.566
PRIMER_RIGHT_3_TM=58.649
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=930
PRIMER_PAIR_4_PENALTY=1.784214
PRIMER_LEFT_4_PENALTY=0.433612
PRIMER_RIGHT_4_PENALTY=1.350602
PRIMER_LEFT_4_SEQUENCE=TCTCAAAGCTCATACGCCAA
PRIMER_RIGHT_4_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_4=535,20
PRIMER_RIGHT_4=1466,20
PRIMER_LEFT_4_TM=59.566
PRIMER_RIGHT_4_TM=58.649
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=932
PRIMER_PAIR_5_PENALTY=1.793268
PRIMER_LEFT_5_PENALTY=0.433612
PRIMER_RIGHT_5_PENALTY=1.359656
PRIMER_LEFT_5_SEQUENCE=TCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_5_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_5=537,20
PRIMER_RIGHT_5=1467,21
PRIMER_LEFT_5_TM=59.566
PRIMER_RIGHT_5_TM=59.640
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=52.381
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=931
PRIMER_PAIR_6_PENALTY=1.793268
PRIMER_LEFT_6_PENALTY=0.433612
PRIMER_RIGHT_6_PENALTY=1.359656
PRIMER_LEFT_6_SEQUENCE=TCTCAAAGCTCATACGCCAA
PRIMER_RIGHT_6_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_6=535,20
PRIMER_RIGHT_6=1467,21
PRIMER_LEFT_6_TM=59.566
PRIMER_RIGHT_6_TM=59.640
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.0000
PRIMER_RIGHT_6_END_STABILITY=9.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=933
PRIMER_PAIR_7_PENALTY=1.812232
PRIMER_LEFT_7_PENALTY=0.433612
PRIMER_RIGHT_7_PENALTY=1.378620
PRIMER_LEFT_7_SEQUENCE=TCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_7_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_7=537,20
PRIMER_RIGHT_7=1468,21
PRIMER_LEFT_7_TM=59.566
PRIMER_RIGHT_7_TM=60.379
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=932
PRIMER_PAIR_8_PENALTY=1.812232
PRIMER_LEFT_8_PENALTY=0.433612
PRIMER_RIGHT_8_PENALTY=1.378620
PRIMER_LEFT_8_SEQUENCE=TCTCAAAGCTCATACGCCAA
PRIMER_RIGHT_8_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_8=535,20
PRIMER_RIGHT_8=1468,21
PRIMER_LEFT_8_TM=59.566
PRIMER_RIGHT_8_TM=60.379
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.0000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=934
PRIMER_PAIR_9_PENALTY=1.847077
PRIMER_LEFT_9_PENALTY=1.324634
PRIMER_RIGHT_9_PENALTY=0.522443
PRIMER_LEFT_9_SEQUENCE=CTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_9_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_9=536,20
PRIMER_RIGHT_9=1465,20
PRIMER_LEFT_9_TM=58.675
PRIMER_RIGHT_9_TM=59.478
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=930
PRIMER_PAIR_10_PENALTY=1.881394
PRIMER_LEFT_10_PENALTY=1.358951
PRIMER_RIGHT_10_PENALTY=0.522443
PRIMER_LEFT_10_SEQUENCE=TGGACTCACTTGAGAATGGG
PRIMER_RIGHT_10_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_10=506,20
PRIMER_RIGHT_10=1465,20
PRIMER_LEFT_10_TM=58.641
PRIMER_RIGHT_10_TM=59.478
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=960
PRIMER_PAIR_11_PENALTY=1.925823
PRIMER_LEFT_11_PENALTY=0.433612
PRIMER_RIGHT_11_PENALTY=1.492211
PRIMER_LEFT_11_SEQUENCE=TCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_11_SEQUENCE=TACAACATAGCCCTTCCGTGT
PRIMER_LEFT_11=537,20
PRIMER_RIGHT_11=1466,21
PRIMER_LEFT_11_TM=59.566
PRIMER_RIGHT_11_TM=59.508
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=930
PRIMER_PAIR_12_PENALTY=1.925823
PRIMER_LEFT_12_PENALTY=0.433612
PRIMER_RIGHT_12_PENALTY=1.492211
PRIMER_LEFT_12_SEQUENCE=TCTCAAAGCTCATACGCCAA
PRIMER_RIGHT_12_SEQUENCE=TACAACATAGCCCTTCCGTGT
PRIMER_LEFT_12=535,20
PRIMER_RIGHT_12=1466,21
PRIMER_LEFT_12_TM=59.566
PRIMER_RIGHT_12_TM=59.508
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.0000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=932
PRIMER_PAIR_13_PENALTY=1.953233
PRIMER_LEFT_13_PENALTY=1.430790
PRIMER_RIGHT_13_PENALTY=0.522443
PRIMER_LEFT_13_SEQUENCE=GATCCAAAACTCGAAGACCC
PRIMER_RIGHT_13_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_13=833,20
PRIMER_RIGHT_13=1465,20
PRIMER_LEFT_13_TM=58.569
PRIMER_RIGHT_13_TM=59.478
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.1000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=633
PRIMER_PAIR_14_PENALTY=1.955087
PRIMER_LEFT_14_PENALTY=1.432644
PRIMER_RIGHT_14_PENALTY=0.522443
PRIMER_LEFT_14_SEQUENCE=GATCCAAAACTCGAAGACCCT
PRIMER_RIGHT_14_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_14=833,21
PRIMER_RIGHT_14=1465,20
PRIMER_LEFT_14_TM=59.567
PRIMER_RIGHT_14_TM=59.478
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=633
PRIMER_PAIR_15_PENALTY=1.991775
PRIMER_LEFT_15_PENALTY=1.469332
PRIMER_RIGHT_15_PENALTY=0.522443
PRIMER_LEFT_15_SEQUENCE=CAAAGCTCATACGCCAAGAGT
PRIMER_RIGHT_15_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_15=538,21
PRIMER_RIGHT_15=1465,20
PRIMER_LEFT_15_TM=59.531
PRIMER_RIGHT_15_TM=59.478
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=928
PRIMER_PAIR_16_PENALTY=2.050869
PRIMER_LEFT_16_PENALTY=1.528426
PRIMER_RIGHT_16_PENALTY=0.522443
PRIMER_LEFT_16_SEQUENCE=CTCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_16_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_16=536,21
PRIMER_RIGHT_16=1465,20
PRIMER_LEFT_16_TM=60.528
PRIMER_RIGHT_16_TM=59.478
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=930
PRIMER_PAIR_17_PENALTY=2.050869
PRIMER_LEFT_17_PENALTY=1.528426
PRIMER_RIGHT_17_PENALTY=0.522443
PRIMER_LEFT_17_SEQUENCE=TCTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_17_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_17=535,21
PRIMER_RIGHT_17=1465,20
PRIMER_LEFT_17_TM=60.528
PRIMER_RIGHT_17_TM=59.478
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.1000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=931
PRIMER_PAIR_18_PENALTY=2.162872
PRIMER_LEFT_18_PENALTY=1.640429
PRIMER_RIGHT_18_PENALTY=0.522443
PRIMER_LEFT_18_SEQUENCE=TGGACTCACTTGAGAATGGGA
PRIMER_RIGHT_18_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_18=506,21
PRIMER_RIGHT_18=1465,20
PRIMER_LEFT_18_TM=60.640
PRIMER_RIGHT_18_TM=59.478
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=960
PRIMER_PAIR_19_PENALTY=2.446494
PRIMER_LEFT_19_PENALTY=1.095892
PRIMER_RIGHT_19_PENALTY=1.350602
PRIMER_LEFT_19_SEQUENCE=TTGGACTCACTTGAGAATGGG
PRIMER_RIGHT_19_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_19=505,21
PRIMER_RIGHT_19=1466,20
PRIMER_LEFT_19_TM=60.096
PRIMER_RIGHT_19_TM=58.649
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=962
PRIMER_PAIR_20_PENALTY=2.455548
PRIMER_LEFT_20_PENALTY=1.095892
PRIMER_RIGHT_20_PENALTY=1.359656
PRIMER_LEFT_20_SEQUENCE=TTGGACTCACTTGAGAATGGG
PRIMER_RIGHT_20_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_20=505,21
PRIMER_RIGHT_20=1467,21
PRIMER_LEFT_20_TM=60.096
PRIMER_RIGHT_20_TM=59.640
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.6000
PRIMER_RIGHT_20_END_STABILITY=9.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=963
PRIMER_PAIR_21_PENALTY=2.474512
PRIMER_LEFT_21_PENALTY=1.095892
PRIMER_RIGHT_21_PENALTY=1.378620
PRIMER_LEFT_21_SEQUENCE=TTGGACTCACTTGAGAATGGG
PRIMER_RIGHT_21_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_21=505,21
PRIMER_RIGHT_21=1468,21
PRIMER_LEFT_21_TM=60.096
PRIMER_RIGHT_21_TM=60.379
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=964
PRIMER_PAIR_22_PENALTY=2.588103
PRIMER_LEFT_22_PENALTY=1.095892
PRIMER_RIGHT_22_PENALTY=1.492211
PRIMER_LEFT_22_SEQUENCE=TTGGACTCACTTGAGAATGGG
PRIMER_RIGHT_22_SEQUENCE=TACAACATAGCCCTTCCGTGT
PRIMER_LEFT_22=505,21
PRIMER_RIGHT_22=1466,21
PRIMER_LEFT_22_TM=60.096
PRIMER_RIGHT_22_TM=59.508
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=962
PRIMER_PAIR_23_PENALTY=2.675236
PRIMER_LEFT_23_PENALTY=1.324634
PRIMER_RIGHT_23_PENALTY=1.350602
PRIMER_LEFT_23_SEQUENCE=CTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_23_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_23=536,20
PRIMER_RIGHT_23=1466,20
PRIMER_LEFT_23_TM=58.675
PRIMER_RIGHT_23_TM=58.649
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=9.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=931
PRIMER_PAIR_24_PENALTY=2.684290
PRIMER_LEFT_24_PENALTY=1.324634
PRIMER_RIGHT_24_PENALTY=1.359656
PRIMER_LEFT_24_SEQUENCE=CTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_24_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_24=536,20
PRIMER_RIGHT_24=1467,21
PRIMER_LEFT_24_TM=58.675
PRIMER_RIGHT_24_TM=59.640
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=9.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=932
PRIMER_PAIR_25_PENALTY=2.703254
PRIMER_LEFT_25_PENALTY=1.324634
PRIMER_RIGHT_25_PENALTY=1.378620
PRIMER_LEFT_25_SEQUENCE=CTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_25_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_25=536,20
PRIMER_RIGHT_25=1468,21
PRIMER_LEFT_25_TM=58.675
PRIMER_RIGHT_25_TM=60.379
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=52.381
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=933
PRIMER_PAIR_26_PENALTY=2.709553
PRIMER_LEFT_26_PENALTY=1.358951
PRIMER_RIGHT_26_PENALTY=1.350602
PRIMER_LEFT_26_SEQUENCE=TGGACTCACTTGAGAATGGG
PRIMER_RIGHT_26_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_26=506,20
PRIMER_RIGHT_26=1466,20
PRIMER_LEFT_26_TM=58.641
PRIMER_RIGHT_26_TM=58.649
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=961
PRIMER_PAIR_27_PENALTY=2.718607
PRIMER_LEFT_27_PENALTY=1.358951
PRIMER_RIGHT_27_PENALTY=1.359656
PRIMER_LEFT_27_SEQUENCE=TGGACTCACTTGAGAATGGG
PRIMER_RIGHT_27_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_27=506,20
PRIMER_RIGHT_27=1467,21
PRIMER_LEFT_27_TM=58.641
PRIMER_RIGHT_27_TM=59.640
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.6000
PRIMER_RIGHT_27_END_STABILITY=9.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=962
PRIMER_PAIR_28_PENALTY=2.737571
PRIMER_LEFT_28_PENALTY=1.358951
PRIMER_RIGHT_28_PENALTY=1.378620
PRIMER_LEFT_28_SEQUENCE=TGGACTCACTTGAGAATGGG
PRIMER_RIGHT_28_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_28=506,20
PRIMER_RIGHT_28=1468,21
PRIMER_LEFT_28_TM=58.641
PRIMER_RIGHT_28_TM=60.379
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.6000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=963
PRIMER_PAIR_29_PENALTY=2.781392
PRIMER_LEFT_29_PENALTY=1.430790
PRIMER_RIGHT_29_PENALTY=1.350602
PRIMER_LEFT_29_SEQUENCE=GATCCAAAACTCGAAGACCC
PRIMER_RIGHT_29_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_29=833,20
PRIMER_RIGHT_29=1466,20
PRIMER_LEFT_29_TM=58.569
PRIMER_RIGHT_29_TM=58.649
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.1000
PRIMER_RIGHT_29_END_STABILITY=9.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=634
PRIMER_PAIR_30_PENALTY=2.783246
PRIMER_LEFT_30_PENALTY=1.432644
PRIMER_RIGHT_30_PENALTY=1.350602
PRIMER_LEFT_30_SEQUENCE=GATCCAAAACTCGAAGACCCT
PRIMER_RIGHT_30_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_30=833,21
PRIMER_RIGHT_30=1466,20
PRIMER_LEFT_30_TM=59.567
PRIMER_RIGHT_30_TM=58.649
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.1000
PRIMER_RIGHT_30_END_STABILITY=9.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=634
PRIMER_PAIR_31_PENALTY=2.790446
PRIMER_LEFT_31_PENALTY=1.430790
PRIMER_RIGHT_31_PENALTY=1.359656
PRIMER_LEFT_31_SEQUENCE=GATCCAAAACTCGAAGACCC
PRIMER_RIGHT_31_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_31=833,20
PRIMER_RIGHT_31=1467,21
PRIMER_LEFT_31_TM=58.569
PRIMER_RIGHT_31_TM=59.640
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.1000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=635
PRIMER_PAIR_32_PENALTY=2.792300
PRIMER_LEFT_32_PENALTY=1.432644
PRIMER_RIGHT_32_PENALTY=1.359656
PRIMER_LEFT_32_SEQUENCE=GATCCAAAACTCGAAGACCCT
PRIMER_RIGHT_32_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_32=833,21
PRIMER_RIGHT_32=1467,21
PRIMER_LEFT_32_TM=59.567
PRIMER_RIGHT_32_TM=59.640
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.1000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=635
PRIMER_PAIR_33_PENALTY=2.809410
PRIMER_LEFT_33_PENALTY=1.430790
PRIMER_RIGHT_33_PENALTY=1.378620
PRIMER_LEFT_33_SEQUENCE=GATCCAAAACTCGAAGACCC
PRIMER_RIGHT_33_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_33=833,20
PRIMER_RIGHT_33=1468,21
PRIMER_LEFT_33_TM=58.569
PRIMER_RIGHT_33_TM=60.379
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.1000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=636
PRIMER_PAIR_34_PENALTY=2.811264
PRIMER_LEFT_34_PENALTY=1.432644
PRIMER_RIGHT_34_PENALTY=1.378620
PRIMER_LEFT_34_SEQUENCE=GATCCAAAACTCGAAGACCCT
PRIMER_RIGHT_34_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_34=833,21
PRIMER_RIGHT_34=1468,21
PRIMER_LEFT_34_TM=59.567
PRIMER_RIGHT_34_TM=60.379
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.1000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=636
PRIMER_PAIR_35_PENALTY=2.814762
PRIMER_LEFT_35_PENALTY=2.292319
PRIMER_RIGHT_35_PENALTY=0.522443
PRIMER_LEFT_35_SEQUENCE=TGATCCAAAACTCGAAGACC
PRIMER_RIGHT_35_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_35=832,20
PRIMER_RIGHT_35=1465,20
PRIMER_LEFT_35_TM=57.708
PRIMER_RIGHT_35_TM=59.478
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.6000
PRIMER_RIGHT_35_END_STABILITY=8.1000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=634
PRIMER_PAIR_36_PENALTY=2.816845
PRIMER_LEFT_36_PENALTY=1.324634
PRIMER_RIGHT_36_PENALTY=1.492211
PRIMER_LEFT_36_SEQUENCE=CTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_36_SEQUENCE=TACAACATAGCCCTTCCGTGT
PRIMER_LEFT_36=536,20
PRIMER_RIGHT_36=1466,21
PRIMER_LEFT_36_TM=58.675
PRIMER_RIGHT_36_TM=59.508
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=8.1000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=931
PRIMER_PAIR_37_PENALTY=2.818299
PRIMER_LEFT_37_PENALTY=2.295856
PRIMER_RIGHT_37_PENALTY=0.522443
PRIMER_LEFT_37_SEQUENCE=ATCCAAAACTCGAAGACCCT
PRIMER_RIGHT_37_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_37=834,20
PRIMER_RIGHT_37=1465,20
PRIMER_LEFT_37_TM=57.704
PRIMER_RIGHT_37_TM=59.478
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.1000
PRIMER_RIGHT_37_END_STABILITY=8.1000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=632
PRIMER_PAIR_38_PENALTY=2.819934
PRIMER_LEFT_38_PENALTY=1.469332
PRIMER_RIGHT_38_PENALTY=1.350602
PRIMER_LEFT_38_SEQUENCE=CAAAGCTCATACGCCAAGAGT
PRIMER_RIGHT_38_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_38=538,21
PRIMER_RIGHT_38=1466,20
PRIMER_LEFT_38_TM=59.531
PRIMER_RIGHT_38_TM=58.649
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.1000
PRIMER_RIGHT_38_END_STABILITY=9.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=929
PRIMER_PAIR_39_PENALTY=2.828988
PRIMER_LEFT_39_PENALTY=1.469332
PRIMER_RIGHT_39_PENALTY=1.359656
PRIMER_LEFT_39_SEQUENCE=CAAAGCTCATACGCCAAGAGT
PRIMER_RIGHT_39_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_39=538,21
PRIMER_RIGHT_39=1467,21
PRIMER_LEFT_39_TM=59.531
PRIMER_RIGHT_39_TM=59.640
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.1000
PRIMER_RIGHT_39_END_STABILITY=9.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=930
PRIMER_PAIR_40_PENALTY=2.847952
PRIMER_LEFT_40_PENALTY=1.469332
PRIMER_RIGHT_40_PENALTY=1.378620
PRIMER_LEFT_40_SEQUENCE=CAAAGCTCATACGCCAAGAGT
PRIMER_RIGHT_40_SEQUENCE=CCTACAACATAGCCCTTCCGT
PRIMER_LEFT_40=538,21
PRIMER_RIGHT_40=1468,21
PRIMER_LEFT_40_TM=59.531
PRIMER_RIGHT_40_TM=60.379
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.1000
PRIMER_RIGHT_40_END_STABILITY=9.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=931
PRIMER_PAIR_41_PENALTY=2.851162
PRIMER_LEFT_41_PENALTY=1.358951
PRIMER_RIGHT_41_PENALTY=1.492211
PRIMER_LEFT_41_SEQUENCE=TGGACTCACTTGAGAATGGG
PRIMER_RIGHT_41_SEQUENCE=TACAACATAGCCCTTCCGTGT
PRIMER_LEFT_41=506,20
PRIMER_RIGHT_41=1466,21
PRIMER_LEFT_41_TM=58.641
PRIMER_RIGHT_41_TM=59.508
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.6000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=961
PRIMER_PAIR_42_PENALTY=2.858004
PRIMER_LEFT_42_PENALTY=0.433612
PRIMER_RIGHT_42_PENALTY=2.424392
PRIMER_LEFT_42_SEQUENCE=TCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_42_SEQUENCE=CTACAACATAGCCCTTCCGTGT
PRIMER_LEFT_42=537,20
PRIMER_RIGHT_42=1467,22
PRIMER_LEFT_42_TM=59.566
PRIMER_RIGHT_42_TM=60.424
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.1000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=931
PRIMER_PAIR_43_PENALTY=2.858004
PRIMER_LEFT_43_PENALTY=0.433612
PRIMER_RIGHT_43_PENALTY=2.424392
PRIMER_LEFT_43_SEQUENCE=TCTCAAAGCTCATACGCCAA
PRIMER_RIGHT_43_SEQUENCE=CTACAACATAGCCCTTCCGTGT
PRIMER_LEFT_43=535,20
PRIMER_RIGHT_43=1467,22
PRIMER_LEFT_43_TM=59.566
PRIMER_RIGHT_43_TM=60.424
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.0000
PRIMER_RIGHT_43_END_STABILITY=8.1000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=933
PRIMER_PAIR_44_PENALTY=2.879028
PRIMER_LEFT_44_PENALTY=1.528426
PRIMER_RIGHT_44_PENALTY=1.350602
PRIMER_LEFT_44_SEQUENCE=CTCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_44_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_44=536,21
PRIMER_RIGHT_44=1466,20
PRIMER_LEFT_44_TM=60.528
PRIMER_RIGHT_44_TM=58.649
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=9.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=931
PRIMER_PAIR_45_PENALTY=2.879028
PRIMER_LEFT_45_PENALTY=1.528426
PRIMER_RIGHT_45_PENALTY=1.350602
PRIMER_LEFT_45_SEQUENCE=TCTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_45_SEQUENCE=TACAACATAGCCCTTCCGTG
PRIMER_LEFT_45=535,21
PRIMER_RIGHT_45=1466,20
PRIMER_LEFT_45_TM=60.528
PRIMER_RIGHT_45_TM=58.649
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=932
PRIMER_PAIR_46_PENALTY=2.888082
PRIMER_LEFT_46_PENALTY=1.528426
PRIMER_RIGHT_46_PENALTY=1.359656
PRIMER_LEFT_46_SEQUENCE=CTCAAAGCTCATACGCCAAGA
PRIMER_RIGHT_46_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_46=536,21
PRIMER_RIGHT_46=1467,21
PRIMER_LEFT_46_TM=60.528
PRIMER_RIGHT_46_TM=59.640
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=932
PRIMER_PAIR_47_PENALTY=2.888082
PRIMER_LEFT_47_PENALTY=1.528426
PRIMER_RIGHT_47_PENALTY=1.359656
PRIMER_LEFT_47_SEQUENCE=TCTCAAAGCTCATACGCCAAG
PRIMER_RIGHT_47_SEQUENCE=CTACAACATAGCCCTTCCGTG
PRIMER_LEFT_47=535,21
PRIMER_RIGHT_47=1467,21
PRIMER_LEFT_47_TM=60.528
PRIMER_RIGHT_47_TM=59.640
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=933
PRIMER_PAIR_48_PENALTY=2.889706
PRIMER_LEFT_48_PENALTY=2.367263
PRIMER_RIGHT_48_PENALTY=0.522443
PRIMER_LEFT_48_SEQUENCE=TGATCCAAAACTCGAAGACCC
PRIMER_RIGHT_48_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_48=832,21
PRIMER_RIGHT_48=1465,20
PRIMER_LEFT_48_TM=61.367
PRIMER_RIGHT_48_TM=59.478
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.1000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=634
PRIMER_PAIR_49_PENALTY=2.906712
PRIMER_LEFT_49_PENALTY=2.384269
PRIMER_RIGHT_49_PENALTY=0.522443
PRIMER_LEFT_49_SEQUENCE=GGACTCACTTGAGAATGGGA
PRIMER_RIGHT_49_SEQUENCE=ACAACATAGCCCTTCCGTGT
PRIMER_LEFT_49=507,20
PRIMER_RIGHT_49=1465,20
PRIMER_LEFT_49_TM=57.616
PRIMER_RIGHT_49_TM=59.478
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=8.1000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=959
=
SEQUENCE_ID=MAGIC.446_4_2585206_2585634_bur-0_tsu-0_5907_4826_TRUE
SEQUENCE_TARGET=950,528
SEQUENCE_TEMPLATE=TTCTTNTCGGATCTACATGTTANNTTTTACACNNTNTGGTCTCAAAACACCAAANATTTGGTTAATGAAGCTATATCGTGTTCATCNTCTAACNTGNTNAGTGTGTTGTCTATTACATTNAATANTTTGCTNNNNNNNNNGTGTGTACTNNNTTTTTTNANGNTTTTNATNTATATTACNTNNGTGGTTTANCTTCTTAATGNAAANCGANGAANGCATCANGAGTATGAGTTCANGTACTTTNAGATACAATTTATTTTTNTTTCTNATGANAGCTATTTACTAATAACTNTNNAGCTATTATCTCTNNTTTTNTTAANATATAAGTGATTGTTATGTGNANACATTGATAAGGAGTATCNNAAGNGCTNATNCNGANATGGTAGAGAGCCTNTTGTNGGAAAANTCAACAACTTCTNCAAAACANNATNNTNNAGAGAATCANGAAAGCTTNNCCANTAGAGTATAAANAAGTGAAGAGTGATCCTCNGTTTNCACACGTCTTTGCCATNTTTGAAANCNNTTTAGGANNNNNNNNNNNTACGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTNNNNNGATGANAANGGGTTTTGGAGNAAGTTNCTTAAGANANGGAACANTATNTNCATTAAGACNTTNATGGATAAGNNNTTATNAGAAGTTCANNNGTGGGATGAAGTNGACNNGGTAAGNTTTGTTTATCTNTGTGTGATTGNNGNATAGTGGTTGCTAGGGATGAGANNNAANATATCAAAGTAAAATACATCAAGTTGGTGATGGANCTTGAGAAGTTGAGAGCATATCNTTGGGNTNTACNTTCGTTNGNNNNNNNTAGTCAATTCCAAAAACNGCAATCTCCCANGATTTNTTCANCAANAATAAATCTCCACTCCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAAGTCACTANTCACTTTANATCCACAATATTCTAGACTTCTAGTAAAAGNCCACANGGACGATGCTTCCAGGNGTTGATNTTTTCNNTGCAGTAACTTTTGGTGTGTGNTTTTANGGGTTTTCGTAACTCTTTCTCAGGAATTTTGANACCATAACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGTGAGGCATNGCTNGGTGNGTCAAGTTTAGATCATTCAGAGGAGATAACCATGAANANTTCTACACTTCGTCCATTAACNAAATCATCAGATTATTCCTTCCTAATTACTAGTCATACCCTAAATATTCACTTACATTAATTTCTGNTANNNNNNNNNNNNNNNNGTCNCTAGANNTTTTTGGGTCNAANTTAAACTTNATCTACTTTATTTCANTAAGATCATNTNTCNNTGTNAGAATGCCAGTTNAATNANCNTTAACACAATCTCTTGACCANNTTTTNTTTNGNNNNNTTGATA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_13890214_13890377_po-0_ler-0_165268_11126_TRUE
SEQUENCE_TARGET=950,263
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGATAATTNNGAGCCTCTAGTGNTTTAAGCACCAAATCTNAACAAACTGTATCAAAAGCTTTGTGAATGTNCAACTTTAGCNNATAATTTTTGGGGCATGTTNTTTTCNGGTAATTCCTGATCAGCTCCGAAGCAAGNATAACATTCTCTNCGAGGCTCNTCCNTTTTAGAAAANNNGCTTGGTTATTGGTTGNCTCGAAANTCAAATCTCTCTCAAGATANTTNAAAAANAAATCCAAAAATAAGAAAGATCTGGAAAAAAAAATTGGATTGATGGANTTNTTANATTTAGAATTTAANAAGATGTATGATTATATTTATTGGTTATAAAGATNTATGNTTATANTATTATTTTAAGTTAATCTTANAAANAAAAGTGGTNTANTATTTATTTTAATCACNNNNNTGGGAAGTTCAATTTANAAAAAAACTGAAAATGTGAAGAAGATAATATACAATAAATTTAGAAACTTTAATNGAATTTAGATTAANNNNNNNNNNCATATCTNAGTTGGCATGTTTTTTATGGATTCTTTTCTAAGNNTTTTTTGATCCNGTCANAAACTTCTTNCATTTNATATAGCCCTATAAAATCCTATGTTTAATCCGANCACNAACCCAATTCACGATCAATAAATGATCGTCNGTTCAGTCTGGCCCTTGATCCNGTTCCCGTAGATATTTTTTTNTTTTTNNAAAATTAGTTATTAGTTTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_6147291_6147333_hi-0_bur-0_707248_99_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=TGANACCAGCGATTACAGAGATGCAAGATCCTCATGTCCCATCTTCTCCAACTATCTCCTCCGTCTCTTCCTCCGATCTTGATACTGAGGTCAGTTCCTGCAATCTTATACTTAATAGATAATCTAATTAAAGATCAAGNNCTTCATGTGTTTTCTAAGCTACNCNNNNNNNNNNATGCTATAAAGTATCAACCTTTAATTAGAAAACTAGTTTAAAGTNCCAAAGGTTATGTTTTCAGAACTGCCCAGAAACATATATATGTACTTGTTAGAACTTGATTTNNTTTTTTNATTGGTGATGTGAGGATTTAGTCNACAGGATCGTTCTTCCATGACAGAAGCATCACACTTGGAACGCTTATGGGATTTAGTTTCACNGCAACTATGCCGATGCCATTNAGAGCNTCTTCACGCCGCCACGTGTCACCTTCCGTCGCAATCTCACGAGCTTCTAGCTCCAATGCAAGACGCAACCACCAGAGAAAGCGTCCTCCTTCAAATTCGGCTGAGCCTGAGCCGCATCGCCGCCGTAAATGGTGGCGGTTTTGCCGAGATGACGATGACGACGCCGCCGGAAATGGAATCCACCGTGGAACAGGAGATTCAAAGCGGTCATCGCTNGGGGAGTATNTNGAGGTGGAACGGAGGTTTGGAGATGAAGCCGTTNACAACTCCGCCGAGGCAGAGCTAGAGGATGCGGTGGTGGCACGGTACCAAGATCAGCAGCCGGTGATGGGGGAGCGGGCATTGTTCGCTGACGGAAGGGTTCTTCCTCCGGCTTCAGNTGAAGNGGTTACCGGAGAANGNACGCCGGTNGCCACGNCCCTTTGTAGATTTCCGGNNTCTTTAACCNGTATATGCAGCGGTGGNGGAGGNTGAATACGTCGGTTCATGTGTGNGNNNNNNATTTGTATTTAGACTTAAATTTTTGTATNNNNNNNNNNNNNNNNGAATCATTGTTTGTGGAAAATATAACATGTTGNATGTAGTCATTGTCAAANTAATTATTTGAGANNNAANNNNNNNTNNNAAGGAGAATTANNAANNANANTANNNANANNNGNAATANTNNNNNNTNNNCNTNNANNANNNNANACNTGNANANCTNTNGNTNNNAANTGGAGNNNNNCNCTNNATNANNNTNTTNATTTNAAATGNTAANNGTTGNTCTATCANATTGAGTTTTTGCTTCAAANNNNNNNNNNNNNNNNTCTTTATACTAGTCAGTCTTTGGACAATAATTTGACCAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANGAGTGTGCCACATTNGTGGTTANTTTTCCACTAGTATTTCTGAAAGTAAAACAATTAACCACAAATCATCCTCATAACATAAGAGGAGAAGATTCCTAACGACGAAGCAAAATTATCGAACAAGTGGACACACACAAGAAACCGAAAATACGGCAAAATGTTTGTTATGTGTATCCATATTTTATANCAATTGAATTAATAAAATAAACTGTANAAACAAGAAACAATTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAANCCTCGAGTGNTGTTAAAAAAAGGAAGAAGGAAAAAGAATAAAAACTCAAAGGGAACGCAACACACTNAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTNNNCAGCAGCAGCAGNAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGCTGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCACCGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCTGAATGATTTTTTCCTTGGGTAGAGTCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.106709
PRIMER_LEFT_0_PENALTY=0.049859
PRIMER_RIGHT_0_PENALTY=0.056850
PRIMER_LEFT_0_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_0_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_0=639,20
PRIMER_RIGHT_0=1444,20
PRIMER_LEFT_0_TM=60.050
PRIMER_RIGHT_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=806
PRIMER_PAIR_1_PENALTY=0.243451
PRIMER_LEFT_1_PENALTY=0.186600
PRIMER_RIGHT_1_PENALTY=0.056850
PRIMER_LEFT_1_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_1_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_1=592,20
PRIMER_RIGHT_1=1444,20
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=853
PRIMER_PAIR_2_PENALTY=0.311463
PRIMER_LEFT_2_PENALTY=0.254613
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_2_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_2=677,20
PRIMER_RIGHT_2=1444,20
PRIMER_LEFT_2_TM=60.255
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=768
PRIMER_PAIR_3_PENALTY=0.410901
PRIMER_LEFT_3_PENALTY=0.254613
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_LEFT_3_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_3_SEQUENCE=GTGTTGCGTTCCCTTTGAGT
PRIMER_LEFT_3=677,20
PRIMER_RIGHT_3=1646,20
PRIMER_LEFT_3_TM=60.255
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=970
PRIMER_PAIR_4_PENALTY=0.422170
PRIMER_LEFT_4_PENALTY=0.365320
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_4_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_4=597,20
PRIMER_RIGHT_4=1444,20
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=848
PRIMER_PAIR_5_PENALTY=0.463402
PRIMER_LEFT_5_PENALTY=0.049859
PRIMER_RIGHT_5_PENALTY=0.413543
PRIMER_LEFT_5_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_5_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_5=639,20
PRIMER_RIGHT_5=1441,20
PRIMER_LEFT_5_TM=60.050
PRIMER_RIGHT_5_TM=59.586
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=803
PRIMER_PAIR_6_PENALTY=0.463402
PRIMER_LEFT_6_PENALTY=0.049859
PRIMER_RIGHT_6_PENALTY=0.413543
PRIMER_LEFT_6_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_6_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_6=639,20
PRIMER_RIGHT_6=1442,20
PRIMER_LEFT_6_TM=60.050
PRIMER_RIGHT_6_TM=59.586
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=804
PRIMER_PAIR_7_PENALTY=0.463402
PRIMER_LEFT_7_PENALTY=0.049859
PRIMER_RIGHT_7_PENALTY=0.413543
PRIMER_LEFT_7_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_7_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_7=639,20
PRIMER_RIGHT_7=1443,20
PRIMER_LEFT_7_TM=60.050
PRIMER_RIGHT_7_TM=59.586
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=805
PRIMER_PAIR_8_PENALTY=0.478724
PRIMER_LEFT_8_PENALTY=0.049859
PRIMER_RIGHT_8_PENALTY=0.428866
PRIMER_LEFT_8_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_8_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_8=639,20
PRIMER_RIGHT_8=1432,20
PRIMER_LEFT_8_TM=60.050
PRIMER_RIGHT_8_TM=59.571
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=794
PRIMER_PAIR_9_PENALTY=0.530362
PRIMER_LEFT_9_PENALTY=0.254613
PRIMER_RIGHT_9_PENALTY=0.275749
PRIMER_LEFT_9_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_9_SEQUENCE=TGCGTTCCCTTTGAGTTTTT
PRIMER_LEFT_9=677,20
PRIMER_RIGHT_9=1642,20
PRIMER_LEFT_9_TM=60.255
PRIMER_RIGHT_9_TM=59.724
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=966
PRIMER_PAIR_10_PENALTY=0.530362
PRIMER_LEFT_10_PENALTY=0.254613
PRIMER_RIGHT_10_PENALTY=0.275749
PRIMER_LEFT_10_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_10_SEQUENCE=TTGCGTTCCCTTTGAGTTTT
PRIMER_LEFT_10=677,20
PRIMER_RIGHT_10=1643,20
PRIMER_LEFT_10_TM=60.255
PRIMER_RIGHT_10_TM=59.724
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=967
PRIMER_PAIR_11_PENALTY=0.530545
PRIMER_LEFT_11_PENALTY=0.473695
PRIMER_RIGHT_11_PENALTY=0.056850
PRIMER_LEFT_11_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_11_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_11=640,20
PRIMER_RIGHT_11=1444,20
PRIMER_LEFT_11_TM=59.526
PRIMER_RIGHT_11_TM=59.943
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=805
PRIMER_PAIR_12_PENALTY=0.567181
PRIMER_LEFT_12_PENALTY=0.510331
PRIMER_RIGHT_12_PENALTY=0.056850
PRIMER_LEFT_12_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_12_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_12=586,20
PRIMER_RIGHT_12=1444,20
PRIMER_LEFT_12_TM=60.510
PRIMER_RIGHT_12_TM=59.943
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=859
PRIMER_PAIR_13_PENALTY=0.600143
PRIMER_LEFT_13_PENALTY=0.186600
PRIMER_RIGHT_13_PENALTY=0.413543
PRIMER_LEFT_13_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_13_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_13=592,20
PRIMER_RIGHT_13=1441,20
PRIMER_LEFT_13_TM=59.813
PRIMER_RIGHT_13_TM=59.586
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=850
PRIMER_PAIR_14_PENALTY=0.600143
PRIMER_LEFT_14_PENALTY=0.186600
PRIMER_RIGHT_14_PENALTY=0.413543
PRIMER_LEFT_14_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_14_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_14=592,20
PRIMER_RIGHT_14=1442,20
PRIMER_LEFT_14_TM=59.813
PRIMER_RIGHT_14_TM=59.586
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=851
PRIMER_PAIR_15_PENALTY=0.600143
PRIMER_LEFT_15_PENALTY=0.186600
PRIMER_RIGHT_15_PENALTY=0.413543
PRIMER_LEFT_15_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_15_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_15=592,20
PRIMER_RIGHT_15=1443,20
PRIMER_LEFT_15_TM=59.813
PRIMER_RIGHT_15_TM=59.586
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=852
PRIMER_PAIR_16_PENALTY=0.615466
PRIMER_LEFT_16_PENALTY=0.186600
PRIMER_RIGHT_16_PENALTY=0.428866
PRIMER_LEFT_16_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_16_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_16=592,20
PRIMER_RIGHT_16=1432,20
PRIMER_LEFT_16_TM=59.813
PRIMER_RIGHT_16_TM=59.571
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=11.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=841
PRIMER_PAIR_17_PENALTY=0.635218
PRIMER_LEFT_17_PENALTY=0.578368
PRIMER_RIGHT_17_PENALTY=0.056850
PRIMER_LEFT_17_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_17_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_17=487,20
PRIMER_RIGHT_17=1444,20
PRIMER_LEFT_17_TM=60.578
PRIMER_RIGHT_17_TM=59.943
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=11.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=958
PRIMER_PAIR_18_PENALTY=0.668156
PRIMER_LEFT_18_PENALTY=0.254613
PRIMER_RIGHT_18_PENALTY=0.413543
PRIMER_LEFT_18_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_18_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_18=677,20
PRIMER_RIGHT_18=1441,20
PRIMER_LEFT_18_TM=60.255
PRIMER_RIGHT_18_TM=59.586
PRIMER_LEFT_18_GC_PERCENT=60.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=765
PRIMER_PAIR_19_PENALTY=0.668156
PRIMER_LEFT_19_PENALTY=0.254613
PRIMER_RIGHT_19_PENALTY=0.413543
PRIMER_LEFT_19_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_19_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_19=677,20
PRIMER_RIGHT_19=1442,20
PRIMER_LEFT_19_TM=60.255
PRIMER_RIGHT_19_TM=59.586
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=766
PRIMER_PAIR_20_PENALTY=0.668156
PRIMER_LEFT_20_PENALTY=0.254613
PRIMER_RIGHT_20_PENALTY=0.413543
PRIMER_LEFT_20_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_20_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_20=677,20
PRIMER_RIGHT_20=1443,20
PRIMER_LEFT_20_TM=60.255
PRIMER_RIGHT_20_TM=59.586
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=767
PRIMER_PAIR_21_PENALTY=0.683479
PRIMER_LEFT_21_PENALTY=0.254613
PRIMER_RIGHT_21_PENALTY=0.428866
PRIMER_LEFT_21_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_21_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_21=677,20
PRIMER_RIGHT_21=1432,20
PRIMER_LEFT_21_TM=60.255
PRIMER_RIGHT_21_TM=59.571
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=756
PRIMER_PAIR_22_PENALTY=0.768641
PRIMER_LEFT_22_PENALTY=0.711791
PRIMER_RIGHT_22_PENALTY=0.056850
PRIMER_LEFT_22_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_22_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_22=594,20
PRIMER_RIGHT_22=1444,20
PRIMER_LEFT_22_TM=59.288
PRIMER_RIGHT_22_TM=59.943
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=851
PRIMER_PAIR_23_PENALTY=0.778863
PRIMER_LEFT_23_PENALTY=0.365320
PRIMER_RIGHT_23_PENALTY=0.413543
PRIMER_LEFT_23_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_23_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_23=597,20
PRIMER_RIGHT_23=1441,20
PRIMER_LEFT_23_TM=59.635
PRIMER_RIGHT_23_TM=59.586
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=845
PRIMER_PAIR_24_PENALTY=0.778863
PRIMER_LEFT_24_PENALTY=0.365320
PRIMER_RIGHT_24_PENALTY=0.413543
PRIMER_LEFT_24_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_24_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_24=597,20
PRIMER_RIGHT_24=1442,20
PRIMER_LEFT_24_TM=59.635
PRIMER_RIGHT_24_TM=59.586
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=846
PRIMER_PAIR_25_PENALTY=0.778863
PRIMER_LEFT_25_PENALTY=0.365320
PRIMER_RIGHT_25_PENALTY=0.413543
PRIMER_LEFT_25_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_25_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_25=597,20
PRIMER_RIGHT_25=1443,20
PRIMER_LEFT_25_TM=59.635
PRIMER_RIGHT_25_TM=59.586
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=847
PRIMER_PAIR_26_PENALTY=0.794186
PRIMER_LEFT_26_PENALTY=0.365320
PRIMER_RIGHT_26_PENALTY=0.428866
PRIMER_LEFT_26_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_26_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_26=597,20
PRIMER_RIGHT_26=1432,20
PRIMER_LEFT_26_TM=59.635
PRIMER_RIGHT_26_TM=59.571
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=836
PRIMER_PAIR_27_PENALTY=0.887238
PRIMER_LEFT_27_PENALTY=0.473695
PRIMER_RIGHT_27_PENALTY=0.413543
PRIMER_LEFT_27_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_27_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_27=640,20
PRIMER_RIGHT_27=1441,20
PRIMER_LEFT_27_TM=59.526
PRIMER_RIGHT_27_TM=59.586
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=802
PRIMER_PAIR_28_PENALTY=0.887238
PRIMER_LEFT_28_PENALTY=0.473695
PRIMER_RIGHT_28_PENALTY=0.413543
PRIMER_LEFT_28_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_28_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_28=640,20
PRIMER_RIGHT_28=1442,20
PRIMER_LEFT_28_TM=59.526
PRIMER_RIGHT_28_TM=59.586
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=803
PRIMER_PAIR_29_PENALTY=0.887238
PRIMER_LEFT_29_PENALTY=0.473695
PRIMER_RIGHT_29_PENALTY=0.413543
PRIMER_LEFT_29_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_29_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_29=640,20
PRIMER_RIGHT_29=1443,20
PRIMER_LEFT_29_TM=59.526
PRIMER_RIGHT_29_TM=59.586
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=804
PRIMER_PAIR_30_PENALTY=0.889843
PRIMER_LEFT_30_PENALTY=0.832993
PRIMER_RIGHT_30_PENALTY=0.056850
PRIMER_LEFT_30_SEQUENCE=CAACCACCAGAGAAAGCGTC
PRIMER_RIGHT_30_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_30=470,20
PRIMER_RIGHT_30=1444,20
PRIMER_LEFT_30_TM=60.833
PRIMER_RIGHT_30_TM=59.943
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=975
PRIMER_PAIR_31_PENALTY=0.889843
PRIMER_LEFT_31_PENALTY=0.832993
PRIMER_RIGHT_31_PENALTY=0.056850
PRIMER_LEFT_31_SEQUENCE=GACGCAACCACCAGAGAAAG
PRIMER_RIGHT_31_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_31=466,20
PRIMER_RIGHT_31=1444,20
PRIMER_LEFT_31_TM=60.833
PRIMER_RIGHT_31_TM=59.943
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=0.889843
PRIMER_LEFT_32_PENALTY=0.832993
PRIMER_RIGHT_32_PENALTY=0.056850
PRIMER_LEFT_32_SEQUENCE=AAGACGCAACCACCAGAGAA
PRIMER_RIGHT_32_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_32=464,20
PRIMER_RIGHT_32=1444,20
PRIMER_LEFT_32_TM=60.833
PRIMER_RIGHT_32_TM=59.943
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=981
PRIMER_PAIR_33_PENALTY=0.902560
PRIMER_LEFT_33_PENALTY=0.473695
PRIMER_RIGHT_33_PENALTY=0.428866
PRIMER_LEFT_33_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_33_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_33=640,20
PRIMER_RIGHT_33=1432,20
PRIMER_LEFT_33_TM=59.526
PRIMER_RIGHT_33_TM=59.571
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=793
PRIMER_PAIR_34_PENALTY=0.919829
PRIMER_LEFT_34_PENALTY=0.862979
PRIMER_RIGHT_34_PENALTY=0.056850
PRIMER_LEFT_34_SEQUENCE=ACGGAGGTTTGGAGATGAAG
PRIMER_RIGHT_34_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_34=641,20
PRIMER_RIGHT_34=1444,20
PRIMER_LEFT_34_TM=59.137
PRIMER_RIGHT_34_TM=59.943
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=804
PRIMER_PAIR_35_PENALTY=0.922928
PRIMER_LEFT_35_PENALTY=0.254613
PRIMER_RIGHT_35_PENALTY=0.668315
PRIMER_LEFT_35_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_35_SEQUENCE=TGTTGCGTTCCCTTTGAGTT
PRIMER_LEFT_35=677,20
PRIMER_RIGHT_35=1645,20
PRIMER_LEFT_35_TM=60.255
PRIMER_RIGHT_35_TM=60.668
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=969
PRIMER_PAIR_36_PENALTY=0.923874
PRIMER_LEFT_36_PENALTY=0.510331
PRIMER_RIGHT_36_PENALTY=0.413543
PRIMER_LEFT_36_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_36_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_36=586,20
PRIMER_RIGHT_36=1441,20
PRIMER_LEFT_36_TM=60.510
PRIMER_RIGHT_36_TM=59.586
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=856
PRIMER_PAIR_37_PENALTY=0.923874
PRIMER_LEFT_37_PENALTY=0.510331
PRIMER_RIGHT_37_PENALTY=0.413543
PRIMER_LEFT_37_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_37_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_37=586,20
PRIMER_RIGHT_37=1442,20
PRIMER_LEFT_37_TM=60.510
PRIMER_RIGHT_37_TM=59.586
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=857
PRIMER_PAIR_38_PENALTY=0.923874
PRIMER_LEFT_38_PENALTY=0.510331
PRIMER_RIGHT_38_PENALTY=0.413543
PRIMER_LEFT_38_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_38_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_38=586,20
PRIMER_RIGHT_38=1443,20
PRIMER_LEFT_38_TM=60.510
PRIMER_RIGHT_38_TM=59.586
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=858
PRIMER_PAIR_39_PENALTY=0.939197
PRIMER_LEFT_39_PENALTY=0.510331
PRIMER_RIGHT_39_PENALTY=0.428866
PRIMER_LEFT_39_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_39_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_39=586,20
PRIMER_RIGHT_39=1432,20
PRIMER_LEFT_39_TM=60.510
PRIMER_RIGHT_39_TM=59.571
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=847
PRIMER_PAIR_40_PENALTY=0.991911
PRIMER_LEFT_40_PENALTY=0.578368
PRIMER_RIGHT_40_PENALTY=0.413543
PRIMER_LEFT_40_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_40_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_40=487,20
PRIMER_RIGHT_40=1441,20
PRIMER_LEFT_40_TM=60.578
PRIMER_RIGHT_40_TM=59.586
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=955
PRIMER_PAIR_41_PENALTY=0.991911
PRIMER_LEFT_41_PENALTY=0.578368
PRIMER_RIGHT_41_PENALTY=0.413543
PRIMER_LEFT_41_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_41_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_41=487,20
PRIMER_RIGHT_41=1442,20
PRIMER_LEFT_41_TM=60.578
PRIMER_RIGHT_41_TM=59.586
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=11.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=956
PRIMER_PAIR_42_PENALTY=0.991911
PRIMER_LEFT_42_PENALTY=0.578368
PRIMER_RIGHT_42_PENALTY=0.413543
PRIMER_LEFT_42_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_42_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_42=487,20
PRIMER_RIGHT_42=1443,20
PRIMER_LEFT_42_TM=60.578
PRIMER_RIGHT_42_TM=59.586
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=11.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=957
PRIMER_PAIR_43_PENALTY=1.007234
PRIMER_LEFT_43_PENALTY=0.578368
PRIMER_RIGHT_43_PENALTY=0.428866
PRIMER_LEFT_43_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_43_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_43=487,20
PRIMER_RIGHT_43=1432,20
PRIMER_LEFT_43_TM=60.578
PRIMER_RIGHT_43_TM=59.571
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=946
PRIMER_PAIR_44_PENALTY=1.036122
PRIMER_LEFT_44_PENALTY=0.254613
PRIMER_RIGHT_44_PENALTY=0.781508
PRIMER_LEFT_44_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_44_SEQUENCE=GTTGCGTTCCCTTTGAGTTT
PRIMER_LEFT_44=677,20
PRIMER_RIGHT_44=1644,20
PRIMER_LEFT_44_TM=60.255
PRIMER_RIGHT_44_TM=59.218
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=968
PRIMER_PAIR_45_PENALTY=1.125334
PRIMER_LEFT_45_PENALTY=0.711791
PRIMER_RIGHT_45_PENALTY=0.413543
PRIMER_LEFT_45_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_45_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_45=594,20
PRIMER_RIGHT_45=1441,20
PRIMER_LEFT_45_TM=59.288
PRIMER_RIGHT_45_TM=59.586
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=848
PRIMER_PAIR_46_PENALTY=1.125334
PRIMER_LEFT_46_PENALTY=0.711791
PRIMER_RIGHT_46_PENALTY=0.413543
PRIMER_LEFT_46_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_46_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_46=594,20
PRIMER_RIGHT_46=1442,20
PRIMER_LEFT_46_TM=59.288
PRIMER_RIGHT_46_TM=59.586
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=849
PRIMER_PAIR_47_PENALTY=1.125334
PRIMER_LEFT_47_PENALTY=0.711791
PRIMER_RIGHT_47_PENALTY=0.413543
PRIMER_LEFT_47_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_47_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_47=594,20
PRIMER_RIGHT_47=1443,20
PRIMER_LEFT_47_TM=59.288
PRIMER_RIGHT_47_TM=59.586
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=850
PRIMER_PAIR_48_PENALTY=1.140657
PRIMER_LEFT_48_PENALTY=0.711791
PRIMER_RIGHT_48_PENALTY=0.428866
PRIMER_LEFT_48_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_48_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_48=594,20
PRIMER_RIGHT_48=1432,20
PRIMER_LEFT_48_TM=59.288
PRIMER_RIGHT_48_TM=59.571
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=839
PRIMER_PAIR_49_PENALTY=1.152585
PRIMER_LEFT_49_PENALTY=1.095734
PRIMER_RIGHT_49_PENALTY=0.056850
PRIMER_LEFT_49_SEQUENCE=CAGAGCTAGAGGATGCGGTG
PRIMER_RIGHT_49_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_49=682,20
PRIMER_RIGHT_49=1444,20
PRIMER_LEFT_49_TM=61.096
PRIMER_RIGHT_49_TM=59.943
PRIMER_LEFT_49_GC_PERCENT=60.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=763
=
SEQUENCE_ID=MAGIC.446_5_17834019_17834433_wu-0_po-0_30826_94027_TRUE
SEQUENCE_TARGET=950,514
SEQUENCE_TEMPLATE=CAGCCAACANTGGAGGCGTATAACCTCTCGTCATCATCTCAACACAATTACACAAAAGCCTCGGNTTCTCTTTTTTGTGTTTCGCAATTATGTTNAGTTTTACGATATGGACACAACATTTTTTACCGGGTTTGTAGTAATATCCCGTGAGAAAGTCTAGACAATAAGAAAAGTAATCATATGTGTATGCACAAAATGGTACGTACGAGTGAACCTGAATCATTAGNCTAGTGTAATCGGAGATCACGAGAAGCAGTAGAAGTGAAATCNCGACTAATTTAGTNGTGTGTGTANCCATTTCTAGANTTCGAATNAATTGATNTACACAATGAGACCAACCTTAGACTAATTTGTACANAGTAGTTCNNNNNNNNTGATCATGCTTATTTCATGAGTTTTTNTTGTTGTTGATGGTTGTNTCATCGTATACGTAAATACCAATGAGAACAATAATACAAATCGAATGAACCTAGGNTTTTATTTTATTTTATNAAACAAAACTCANNAATAAACCTANCNGGAGGATAATTAATAGCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNTCTAAAGNGTAGTTAATATNTTCTTATANTCNTANTATGTNNATGTGAATTTTCTTNTNNTNTGATNTTTGTGAGANGCNAAAATCNTCCTACCATTANTAAATNTACATTTTNTTGTCNTTTAAGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATAAAGTTTGAATCTNGTTTCATTTAGATTGTTGTAATTGTGAGTAAANAAAAGATTAAAAANTAATACAAAATATTTNACCTCTATTTGATGGCTCACNTTANTNNNATTATTNNNNNNNNNNNNNNNNNNTAGAGACTGTANTATCTTGCTTGTTTTGCATAACTCTAAATCAAGACAGGTGAAGCCATCAAAAGGCAAAAGCTTGATGATGGGAGGTNAATCCANACAAGTTAGTCAACTCAAACTGCAATACTTGTTTCACTATCTCACTGTATNNGTTAATCCGACCGTTGATGTCACTAGATTCTGAACCNAAAACCAACAACTTTGCCTCACAAGANAAAANNNNGACTTGTTAACACGAGCTTCAACTTCTGCCCTTCAGTCACTAAGCAAACCCGGACAGAGAACATAAAGGTGCATTGNNTCTGCCGATGACACTAAAAAGGCGAAAGNATTCAGAGTTTGTGACATCTCAGAGAGTTCGTCCTGTAAGACTNAANGNACTTCAGAGCTNGAGGAAGAGATGTTGGCAAAGATNCCTNAGTTNAAAGNGCGACCNTNGAATAAGAAGGTACTGCGTGGCTTTCCCACAACGTCTCTCTCTTCTGTAACTTNAGAAACAACAGAGCTGNACAGAAATNAGAAAACTGAAAACTAAGCATGCGCTTCCTCTCTGTTTCACANAGATTTTGGCNGCTCCAGCTNTGCCCACACCACAAAGAAGTANNNCACATCCTCCAGAATTTCANGTNAACCACACTGATCAGACCANGAGATTCAAACNCTCTNATCCAAAATATTTTNTGAGTGAGANNTTTTAAACGTGGCTTTATTCTTACTGTTGCAGGAGTTCCATCTAGAAACNATGGNNCGGNCTAGCCGCTAGCCCGTANGGAGAGAGACATCTTCAGTAGTNTCGACGGAAATGTCTCAGGCTATAGAGCTTTTGTTTGTTTCCTATTGTCTTCAGNGCNCAGCCAGTGATTAAAGAGTAAGTGANTGTTCNTCTGCTGTTTCTTCNTCAACATATACAGTTTCNTTTANATATTCAAAGACNCAAATAANTTCTGTGAATTCTCANNNNNNNNNCTGACTCAGAGATCAAANTTCTGTTCTNGATAAACTAAAGGCCTCTTANAGAATTTTTCAAGAGTTTAATCTGCATGTGGACCATCGGCCTNTCGAAAGGGCANANTTTGANCNNAAAGTAAGCTTATCTAACTCGATCAGCAGATTAAAAGAAACCTTTTGATTTCTCAACTAACTTGTTNTATCATCTTTGGTTCCAGATCAAAGAGAAANAGATGATNTACAAGAGANATCNCNAAGAGGCGGAAGCCGCANAAATGGTATAATACTNTANATTTTCTGCCAAGTATANATGTATCTANTAAAAGAGNATGATAGTAAATGTATTTTACCT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_8528056_8528202_po-0_edi-0_117458_23744_TRUE
SEQUENCE_TARGET=950,246
SEQUENCE_TEMPLATE=AAANTTAGTTTTTTTCATATTACCATGCATNTTTTTTNNGGCCCATATAAGCCCAATATTACCGTGCAAGTTTTTTCTTATTTTTTAAATTTTATCTGAAAGTAAAATTGAACAGAGTANCGATGACGTGTCCTAACTCCCAAGNAGAAGAAGAAGAAGAAGNNNAAAAAAAANNAAAAAGATNTGAAAGAAATAAAAAACGAATCGGANNNNNNNNAGAGAGAGAGANNNNNNNNNNNNTTGGAGATGGGAATAAGGTTCATATTGATGGTGAACAAGCAAGGNCAGACTCGTCTTGCTCAGTACTACGAATGGCTCACTCTCGAGGAACGTCGCGCTCTCGAAGGCGAAATCGTNCGTAAATGNCTCGCTCGCAACGACCAACANNNNNNNNNNNNNNNNNNNNNNNNNNTTTCNATTAGATCGCTCCTGATTCTCCTCCGATCTCNTNTCATTGATATCTATTTTGATCTCCTTAGTTTTGNNNNNNNNNNNNNNNNNNNTGATCTGGATATGGATCGATCNGGCATTAGGTTTATTNCTTCTNGATANATTTGGNAATCGAAGTAATTTTGTTGGAAATCTAGATTGATACATAGCCGATTTTGGGAANTTTCGTAGCGGTGATGCATANATCACCGACCATTGGCNAATTGGATTCAAACTAATTTAGTATCATTGATCCNAATTACATTNAGGTCTTNGTTAATTCTGTTTAGCTTANGGCATCTGACTANATTTTNNTGCATTTGNTNTTAATAAACAGTGTTCGTTTGTCGAGCATCGCAACTACAAGATAGTCTACAGGCGTTACGCNTCTCTNTTCTTCATGGTTGGGGTTGATGACGATGAAGTAAGTTTGNTATACATCACTGTTTTCNTATTGAGCTGCATACAATNTAGAANANTGTTTNTGATTGATNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNACAATNNNNNNNNNNTNNTNNTNNANACNANNCTCTCTNCTCTCTGANTCTTGTCNTAGAACGAGCTGGCGATTCTAGAGTTCATNCATCTTTTGGTNGAGACAATGGACAAGCATTTTGGAAATGTGGTAAGCTGTNTNGGTTCATANTAGTCTCGAATCGAATAGACTCNCTTGNTTTTTGTGTTTCCCANTTTGTGATTTATATAAACTCTTTTTTATTTTATGCAGTGTGAGCTAGACATAATGTTCCATCTGGAGAAAGCTCATTTCATGCTGGAAGAAATGGTGATGAATGGTTGCATTGTGGAGACAAGCAAAGCCAACATACTTTCACCTATACAACTTATGGACAAAGCCCATTAATGCTAAACACCCTGTTTCTATTTCTGTTAATCCGTTTCATCACTTTGTTACACACTTCTTCTTGTATTATTATTAACGATGCTTCAAAGAGAGGATAATACAAAAGAAGCANGAAATCGAAAAGTACCAATTGGGAGTACAATTCACAAGAACCCGATGATAATAAGGATAGTTACATTGTCTAGCCATTACATCCGCANATAGGTCTGTTTGTCGACTTCTTGAAAAAAACGAATGGCTGAAAAGTGTCTTCTTGGATTCCTTAAGATCTCTTTTGTGTATGTTTTCAGCCTTGGCCATTTGGTTGGATCATCAATAGGTTGAACCAATTGCTTAGAGTCACTGTCAAACTGAACATACTTATATTCCAAGTTGTCCAAGCATGACCTTGTCCANACTAGTGTCCTAGCTAGTACCTACCACTTCCATGTTTTAACCACAATCTTCAGAAAGTCTTGTCACCTTACCACTCTTTTTNAATCTTCTAGTATCCAACCAACTCCACATATTGTTGAATCTGCATTTAGGTTTTACCTNTCAATAATCAATGACTTTACCAATAATCAATGACTTTAGNTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNATTTNATTATTTGTTGATGTTAGGTTGTTAGATTTTGGATTTTATTATTTTTCTTCATANATGGTTGTTGTCAACGCTTANTCCAGAATGATTACCTAGN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.237695
PRIMER_LEFT_0_PENALTY=0.018700
PRIMER_RIGHT_0_PENALTY=0.218996
PRIMER_LEFT_0_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_0_SEQUENCE=TGCAACCATTCATCACCATT
PRIMER_LEFT_0=775,20
PRIMER_RIGHT_0=1277,20
PRIMER_LEFT_0_TM=60.019
PRIMER_RIGHT_0_TM=59.781
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=503
PRIMER_PAIR_1_PENALTY=0.237695
PRIMER_LEFT_1_PENALTY=0.018700
PRIMER_RIGHT_1_PENALTY=0.218996
PRIMER_LEFT_1_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_1_SEQUENCE=AATGCAACCATTCATCACCA
PRIMER_LEFT_1=775,20
PRIMER_RIGHT_1=1279,20
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=59.781
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=505
PRIMER_PAIR_2_PENALTY=0.237695
PRIMER_LEFT_2_PENALTY=0.018700
PRIMER_RIGHT_2_PENALTY=0.218996
PRIMER_LEFT_2_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_2_SEQUENCE=CAATGCAACCATTCATCACC
PRIMER_LEFT_2=775,20
PRIMER_RIGHT_2=1280,20
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=59.781
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=506
PRIMER_PAIR_3_PENALTY=0.237695
PRIMER_LEFT_3_PENALTY=0.018700
PRIMER_RIGHT_3_PENALTY=0.218996
PRIMER_LEFT_3_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_3_SEQUENCE=CCACAATGCAACCATTCATC
PRIMER_LEFT_3=775,20
PRIMER_RIGHT_3=1283,20
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=59.781
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=509
PRIMER_PAIR_4_PENALTY=0.237695
PRIMER_LEFT_4_PENALTY=0.018700
PRIMER_RIGHT_4_PENALTY=0.218996
PRIMER_LEFT_4_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_4_SEQUENCE=TGCAACCATTCATCACCATT
PRIMER_LEFT_4=773,20
PRIMER_RIGHT_4=1277,20
PRIMER_LEFT_4_TM=60.019
PRIMER_RIGHT_4_TM=59.781
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=505
PRIMER_PAIR_5_PENALTY=0.237695
PRIMER_LEFT_5_PENALTY=0.018700
PRIMER_RIGHT_5_PENALTY=0.218996
PRIMER_LEFT_5_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_5_SEQUENCE=AATGCAACCATTCATCACCA
PRIMER_LEFT_5=773,20
PRIMER_RIGHT_5=1279,20
PRIMER_LEFT_5_TM=60.019
PRIMER_RIGHT_5_TM=59.781
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.1000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=507
PRIMER_PAIR_6_PENALTY=0.237695
PRIMER_LEFT_6_PENALTY=0.018700
PRIMER_RIGHT_6_PENALTY=0.218996
PRIMER_LEFT_6_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_6_SEQUENCE=CAATGCAACCATTCATCACC
PRIMER_LEFT_6=773,20
PRIMER_RIGHT_6=1280,20
PRIMER_LEFT_6_TM=60.019
PRIMER_RIGHT_6_TM=59.781
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.1000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=508
PRIMER_PAIR_7_PENALTY=0.237695
PRIMER_LEFT_7_PENALTY=0.018700
PRIMER_RIGHT_7_PENALTY=0.218996
PRIMER_LEFT_7_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_7_SEQUENCE=CCACAATGCAACCATTCATC
PRIMER_LEFT_7=773,20
PRIMER_RIGHT_7=1283,20
PRIMER_LEFT_7_TM=60.019
PRIMER_RIGHT_7_TM=59.781
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=5.1000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=511
PRIMER_PAIR_8_PENALTY=0.370510
PRIMER_LEFT_8_PENALTY=0.018700
PRIMER_RIGHT_8_PENALTY=0.351810
PRIMER_LEFT_8_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_8_SEQUENCE=CATCACCATTTCTTCCAGCA
PRIMER_LEFT_8=775,20
PRIMER_RIGHT_8=1267,20
PRIMER_LEFT_8_TM=60.019
PRIMER_RIGHT_8_TM=59.648
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=2.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=493
PRIMER_PAIR_9_PENALTY=0.370510
PRIMER_LEFT_9_PENALTY=0.018700
PRIMER_RIGHT_9_PENALTY=0.351810
PRIMER_LEFT_9_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_9_SEQUENCE=CATCACCATTTCTTCCAGCA
PRIMER_LEFT_9=773,20
PRIMER_RIGHT_9=1267,20
PRIMER_LEFT_9_TM=60.019
PRIMER_RIGHT_9_TM=59.648
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=5.1000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=2.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=495
PRIMER_PAIR_10_PENALTY=0.376236
PRIMER_LEFT_10_PENALTY=0.157240
PRIMER_RIGHT_10_PENALTY=0.218996
PRIMER_LEFT_10_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_10_SEQUENCE=TGCAACCATTCATCACCATT
PRIMER_LEFT_10=777,20
PRIMER_RIGHT_10=1277,20
PRIMER_LEFT_10_TM=60.157
PRIMER_RIGHT_10_TM=59.781
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=501
PRIMER_PAIR_11_PENALTY=0.376236
PRIMER_LEFT_11_PENALTY=0.157240
PRIMER_RIGHT_11_PENALTY=0.218996
PRIMER_LEFT_11_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_11_SEQUENCE=AATGCAACCATTCATCACCA
PRIMER_LEFT_11=777,20
PRIMER_RIGHT_11=1279,20
PRIMER_LEFT_11_TM=60.157
PRIMER_RIGHT_11_TM=59.781
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=503
PRIMER_PAIR_12_PENALTY=0.376236
PRIMER_LEFT_12_PENALTY=0.157240
PRIMER_RIGHT_12_PENALTY=0.218996
PRIMER_LEFT_12_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_12_SEQUENCE=CAATGCAACCATTCATCACC
PRIMER_LEFT_12=777,20
PRIMER_RIGHT_12=1280,20
PRIMER_LEFT_12_TM=60.157
PRIMER_RIGHT_12_TM=59.781
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=504
PRIMER_PAIR_13_PENALTY=0.376236
PRIMER_LEFT_13_PENALTY=0.157240
PRIMER_RIGHT_13_PENALTY=0.218996
PRIMER_LEFT_13_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_13_SEQUENCE=CCACAATGCAACCATTCATC
PRIMER_LEFT_13=777,20
PRIMER_RIGHT_13=1283,20
PRIMER_LEFT_13_TM=60.157
PRIMER_RIGHT_13_TM=59.781
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=507
PRIMER_PAIR_14_PENALTY=0.376236
PRIMER_LEFT_14_PENALTY=0.157240
PRIMER_RIGHT_14_PENALTY=0.218996
PRIMER_LEFT_14_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_14_SEQUENCE=TGCAACCATTCATCACCATT
PRIMER_LEFT_14=776,20
PRIMER_RIGHT_14=1277,20
PRIMER_LEFT_14_TM=60.157
PRIMER_RIGHT_14_TM=59.781
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=502
PRIMER_PAIR_15_PENALTY=0.376236
PRIMER_LEFT_15_PENALTY=0.157240
PRIMER_RIGHT_15_PENALTY=0.218996
PRIMER_LEFT_15_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_15_SEQUENCE=AATGCAACCATTCATCACCA
PRIMER_LEFT_15=776,20
PRIMER_RIGHT_15=1279,20
PRIMER_LEFT_15_TM=60.157
PRIMER_RIGHT_15_TM=59.781
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=504
PRIMER_PAIR_16_PENALTY=0.376236
PRIMER_LEFT_16_PENALTY=0.157240
PRIMER_RIGHT_16_PENALTY=0.218996
PRIMER_LEFT_16_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_16_SEQUENCE=CAATGCAACCATTCATCACC
PRIMER_LEFT_16=776,20
PRIMER_RIGHT_16=1280,20
PRIMER_LEFT_16_TM=60.157
PRIMER_RIGHT_16_TM=59.781
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=505
PRIMER_PAIR_17_PENALTY=0.376236
PRIMER_LEFT_17_PENALTY=0.157240
PRIMER_RIGHT_17_PENALTY=0.218996
PRIMER_LEFT_17_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_17_SEQUENCE=CCACAATGCAACCATTCATC
PRIMER_LEFT_17=776,20
PRIMER_RIGHT_17=1283,20
PRIMER_LEFT_17_TM=60.157
PRIMER_RIGHT_17_TM=59.781
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=508
PRIMER_PAIR_18_PENALTY=0.444509
PRIMER_LEFT_18_PENALTY=0.018700
PRIMER_RIGHT_18_PENALTY=0.425809
PRIMER_LEFT_18_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_18_SEQUENCE=GGCCAAGGCTGAAAACATAC
PRIMER_LEFT_18=775,20
PRIMER_RIGHT_18=1637,20
PRIMER_LEFT_18_TM=60.019
PRIMER_RIGHT_18_TM=59.574
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.0000
PRIMER_RIGHT_18_END_STABILITY=5.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=863
PRIMER_PAIR_19_PENALTY=0.444509
PRIMER_LEFT_19_PENALTY=0.018700
PRIMER_RIGHT_19_PENALTY=0.425809
PRIMER_LEFT_19_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_19_SEQUENCE=GGCCAAGGCTGAAAACATAC
PRIMER_LEFT_19=773,20
PRIMER_RIGHT_19=1637,20
PRIMER_LEFT_19_TM=60.019
PRIMER_RIGHT_19_TM=59.574
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.1000
PRIMER_RIGHT_19_END_STABILITY=5.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=865
PRIMER_PAIR_20_PENALTY=0.509050
PRIMER_LEFT_20_PENALTY=0.157240
PRIMER_RIGHT_20_PENALTY=0.351810
PRIMER_LEFT_20_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_20_SEQUENCE=CATCACCATTTCTTCCAGCA
PRIMER_LEFT_20=777,20
PRIMER_RIGHT_20=1267,20
PRIMER_LEFT_20_TM=60.157
PRIMER_RIGHT_20_TM=59.648
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=491
PRIMER_PAIR_21_PENALTY=0.509050
PRIMER_LEFT_21_PENALTY=0.157240
PRIMER_RIGHT_21_PENALTY=0.351810
PRIMER_LEFT_21_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_21_SEQUENCE=CATCACCATTTCTTCCAGCA
PRIMER_LEFT_21=776,20
PRIMER_RIGHT_21=1267,20
PRIMER_LEFT_21_TM=60.157
PRIMER_RIGHT_21_TM=59.648
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=492
PRIMER_PAIR_22_PENALTY=0.535418
PRIMER_LEFT_22_PENALTY=0.018700
PRIMER_RIGHT_22_PENALTY=0.516718
PRIMER_LEFT_22_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_22_SEQUENCE=CTCCACAATGCAACCATTCA
PRIMER_LEFT_22=775,20
PRIMER_RIGHT_22=1285,20
PRIMER_LEFT_22_TM=60.019
PRIMER_RIGHT_22_TM=60.517
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.0000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=511
PRIMER_PAIR_23_PENALTY=0.535418
PRIMER_LEFT_23_PENALTY=0.018700
PRIMER_RIGHT_23_PENALTY=0.516718
PRIMER_LEFT_23_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_23_SEQUENCE=CTCCACAATGCAACCATTCA
PRIMER_LEFT_23=773,20
PRIMER_RIGHT_23=1285,20
PRIMER_LEFT_23_TM=60.019
PRIMER_RIGHT_23_TM=60.517
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=5.1000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=513
PRIMER_PAIR_24_PENALTY=0.566174
PRIMER_LEFT_24_PENALTY=0.214364
PRIMER_RIGHT_24_PENALTY=0.351810
PRIMER_LEFT_24_SEQUENCE=GGGGTTGATGACGATGAAGT
PRIMER_RIGHT_24_SEQUENCE=CATCACCATTTCTTCCAGCA
PRIMER_LEFT_24=835,20
PRIMER_RIGHT_24=1267,20
PRIMER_LEFT_24_TM=59.786
PRIMER_RIGHT_24_TM=59.648
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=433
PRIMER_PAIR_25_PENALTY=0.583049
PRIMER_LEFT_25_PENALTY=0.157240
PRIMER_RIGHT_25_PENALTY=0.425809
PRIMER_LEFT_25_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_25_SEQUENCE=GGCCAAGGCTGAAAACATAC
PRIMER_LEFT_25=777,20
PRIMER_RIGHT_25=1637,20
PRIMER_LEFT_25_TM=60.157
PRIMER_RIGHT_25_TM=59.574
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=5.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=861
PRIMER_PAIR_26_PENALTY=0.583049
PRIMER_LEFT_26_PENALTY=0.157240
PRIMER_RIGHT_26_PENALTY=0.425809
PRIMER_LEFT_26_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_26_SEQUENCE=GGCCAAGGCTGAAAACATAC
PRIMER_LEFT_26=776,20
PRIMER_RIGHT_26=1637,20
PRIMER_LEFT_26_TM=60.157
PRIMER_RIGHT_26_TM=59.574
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=5.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=862
PRIMER_PAIR_27_PENALTY=0.640173
PRIMER_LEFT_27_PENALTY=0.214364
PRIMER_RIGHT_27_PENALTY=0.425809
PRIMER_LEFT_27_SEQUENCE=GGGGTTGATGACGATGAAGT
PRIMER_RIGHT_27_SEQUENCE=GGCCAAGGCTGAAAACATAC
PRIMER_LEFT_27=835,20
PRIMER_RIGHT_27=1637,20
PRIMER_LEFT_27_TM=59.786
PRIMER_RIGHT_27_TM=59.574
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=5.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=803
PRIMER_PAIR_28_PENALTY=0.673958
PRIMER_LEFT_28_PENALTY=0.157240
PRIMER_RIGHT_28_PENALTY=0.516718
PRIMER_LEFT_28_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_28_SEQUENCE=CTCCACAATGCAACCATTCA
PRIMER_LEFT_28=777,20
PRIMER_RIGHT_28=1285,20
PRIMER_LEFT_28_TM=60.157
PRIMER_RIGHT_28_TM=60.517
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=509
PRIMER_PAIR_29_PENALTY=0.673958
PRIMER_LEFT_29_PENALTY=0.157240
PRIMER_RIGHT_29_PENALTY=0.516718
PRIMER_LEFT_29_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_29_SEQUENCE=CTCCACAATGCAACCATTCA
PRIMER_LEFT_29=776,20
PRIMER_RIGHT_29=1285,20
PRIMER_LEFT_29_TM=60.157
PRIMER_RIGHT_29_TM=60.517
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=510
PRIMER_PAIR_30_PENALTY=0.721447
PRIMER_LEFT_30_PENALTY=0.018700
PRIMER_RIGHT_30_PENALTY=0.702747
PRIMER_LEFT_30_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_30_SEQUENCE=GCTTGTCTCCACAATGCAAC
PRIMER_LEFT_30=775,20
PRIMER_RIGHT_30=1291,20
PRIMER_LEFT_30_TM=60.019
PRIMER_RIGHT_30_TM=59.297
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.0000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=517
PRIMER_PAIR_31_PENALTY=0.721447
PRIMER_LEFT_31_PENALTY=0.018700
PRIMER_RIGHT_31_PENALTY=0.702747
PRIMER_LEFT_31_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_31_SEQUENCE=GCTTGTCTCCACAATGCAAC
PRIMER_LEFT_31=773,20
PRIMER_RIGHT_31=1291,20
PRIMER_LEFT_31_TM=60.019
PRIMER_RIGHT_31_TM=59.297
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=5.1000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=519
PRIMER_PAIR_32_PENALTY=0.731082
PRIMER_LEFT_32_PENALTY=0.214364
PRIMER_RIGHT_32_PENALTY=0.516718
PRIMER_LEFT_32_SEQUENCE=GGGGTTGATGACGATGAAGT
PRIMER_RIGHT_32_SEQUENCE=CTCCACAATGCAACCATTCA
PRIMER_LEFT_32=835,20
PRIMER_RIGHT_32=1285,20
PRIMER_LEFT_32_TM=59.786
PRIMER_RIGHT_32_TM=60.517
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=451
PRIMER_PAIR_33_PENALTY=0.859987
PRIMER_LEFT_33_PENALTY=0.157240
PRIMER_RIGHT_33_PENALTY=0.702747
PRIMER_LEFT_33_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_33_SEQUENCE=GCTTGTCTCCACAATGCAAC
PRIMER_LEFT_33=777,20
PRIMER_RIGHT_33=1291,20
PRIMER_LEFT_33_TM=60.157
PRIMER_RIGHT_33_TM=59.297
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=515
PRIMER_PAIR_34_PENALTY=0.859987
PRIMER_LEFT_34_PENALTY=0.157240
PRIMER_RIGHT_34_PENALTY=0.702747
PRIMER_LEFT_34_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_34_SEQUENCE=GCTTGTCTCCACAATGCAAC
PRIMER_LEFT_34=776,20
PRIMER_RIGHT_34=1291,20
PRIMER_LEFT_34_TM=60.157
PRIMER_RIGHT_34_TM=59.297
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=516
PRIMER_PAIR_35_PENALTY=0.879863
PRIMER_LEFT_35_PENALTY=0.018700
PRIMER_RIGHT_35_PENALTY=0.861164
PRIMER_LEFT_35_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_35_SEQUENCE=CTTGTCTCCACAATGCAACC
PRIMER_LEFT_35=775,20
PRIMER_RIGHT_35=1290,20
PRIMER_LEFT_35_TM=60.019
PRIMER_RIGHT_35_TM=59.139
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.0000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=516
PRIMER_PAIR_36_PENALTY=0.879863
PRIMER_LEFT_36_PENALTY=0.018700
PRIMER_RIGHT_36_PENALTY=0.861164
PRIMER_LEFT_36_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_36_SEQUENCE=CTTGTCTCCACAATGCAACC
PRIMER_LEFT_36=773,20
PRIMER_RIGHT_36=1290,20
PRIMER_LEFT_36_TM=60.019
PRIMER_RIGHT_36_TM=59.139
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.1000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=518
PRIMER_PAIR_37_PENALTY=0.917111
PRIMER_LEFT_37_PENALTY=0.214364
PRIMER_RIGHT_37_PENALTY=0.702747
PRIMER_LEFT_37_SEQUENCE=GGGGTTGATGACGATGAAGT
PRIMER_RIGHT_37_SEQUENCE=GCTTGTCTCCACAATGCAAC
PRIMER_LEFT_37=835,20
PRIMER_RIGHT_37=1291,20
PRIMER_LEFT_37_TM=59.786
PRIMER_RIGHT_37_TM=59.297
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=457
PRIMER_PAIR_38_PENALTY=0.922267
PRIMER_LEFT_38_PENALTY=0.703271
PRIMER_RIGHT_38_PENALTY=0.218996
PRIMER_LEFT_38_SEQUENCE=ATCGCTCCTGATTCTCCTCC
PRIMER_RIGHT_38_SEQUENCE=TGCAACCATTCATCACCATT
PRIMER_LEFT_38=422,20
PRIMER_RIGHT_38=1277,20
PRIMER_LEFT_38_TM=60.703
PRIMER_RIGHT_38_TM=59.781
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=856
PRIMER_PAIR_39_PENALTY=0.922267
PRIMER_LEFT_39_PENALTY=0.703271
PRIMER_RIGHT_39_PENALTY=0.218996
PRIMER_LEFT_39_SEQUENCE=ATCGCTCCTGATTCTCCTCC
PRIMER_RIGHT_39_SEQUENCE=AATGCAACCATTCATCACCA
PRIMER_LEFT_39=422,20
PRIMER_RIGHT_39=1279,20
PRIMER_LEFT_39_TM=60.703
PRIMER_RIGHT_39_TM=59.781
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=858
PRIMER_PAIR_40_PENALTY=0.922267
PRIMER_LEFT_40_PENALTY=0.703271
PRIMER_RIGHT_40_PENALTY=0.218996
PRIMER_LEFT_40_SEQUENCE=ATCGCTCCTGATTCTCCTCC
PRIMER_RIGHT_40_SEQUENCE=CAATGCAACCATTCATCACC
PRIMER_LEFT_40=422,20
PRIMER_RIGHT_40=1280,20
PRIMER_LEFT_40_TM=60.703
PRIMER_RIGHT_40_TM=59.781
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=859
PRIMER_PAIR_41_PENALTY=0.922267
PRIMER_LEFT_41_PENALTY=0.703271
PRIMER_RIGHT_41_PENALTY=0.218996
PRIMER_LEFT_41_SEQUENCE=ATCGCTCCTGATTCTCCTCC
PRIMER_RIGHT_41_SEQUENCE=CCACAATGCAACCATTCATC
PRIMER_LEFT_41=422,20
PRIMER_RIGHT_41=1283,20
PRIMER_LEFT_41_TM=60.703
PRIMER_RIGHT_41_TM=59.781
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=6.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=862
PRIMER_PAIR_42_PENALTY=1.018404
PRIMER_LEFT_42_PENALTY=0.157240
PRIMER_RIGHT_42_PENALTY=0.861164
PRIMER_LEFT_42_SEQUENCE=CGAGCATCGCAACTACAAGA
PRIMER_RIGHT_42_SEQUENCE=CTTGTCTCCACAATGCAACC
PRIMER_LEFT_42=777,20
PRIMER_RIGHT_42=1290,20
PRIMER_LEFT_42_TM=60.157
PRIMER_RIGHT_42_TM=59.139
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=514
PRIMER_PAIR_43_PENALTY=1.018404
PRIMER_LEFT_43_PENALTY=0.157240
PRIMER_RIGHT_43_PENALTY=0.861164
PRIMER_LEFT_43_SEQUENCE=TCGAGCATCGCAACTACAAG
PRIMER_RIGHT_43_SEQUENCE=CTTGTCTCCACAATGCAACC
PRIMER_LEFT_43=776,20
PRIMER_RIGHT_43=1290,20
PRIMER_LEFT_43_TM=60.157
PRIMER_RIGHT_43_TM=59.139
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=515
PRIMER_PAIR_44_PENALTY=1.055082
PRIMER_LEFT_44_PENALTY=0.703271
PRIMER_RIGHT_44_PENALTY=0.351810
PRIMER_LEFT_44_SEQUENCE=ATCGCTCCTGATTCTCCTCC
PRIMER_RIGHT_44_SEQUENCE=CATCACCATTTCTTCCAGCA
PRIMER_LEFT_44=422,20
PRIMER_RIGHT_44=1267,20
PRIMER_LEFT_44_TM=60.703
PRIMER_RIGHT_44_TM=59.648
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=846
PRIMER_PAIR_45_PENALTY=1.147487
PRIMER_LEFT_45_PENALTY=0.018700
PRIMER_RIGHT_45_PENALTY=1.128787
PRIMER_LEFT_45_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_45_SEQUENCE=TTGTCTCCACAATGCAACCA
PRIMER_LEFT_45=775,20
PRIMER_RIGHT_45=1289,20
PRIMER_LEFT_45_TM=60.019
PRIMER_RIGHT_45_TM=61.129
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.0000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=515
PRIMER_PAIR_46_PENALTY=1.147487
PRIMER_LEFT_46_PENALTY=0.018700
PRIMER_RIGHT_46_PENALTY=1.128787
PRIMER_LEFT_46_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_46_SEQUENCE=TTGTCTCCACAATGCAACCA
PRIMER_LEFT_46=773,20
PRIMER_RIGHT_46=1289,20
PRIMER_LEFT_46_TM=60.019
PRIMER_RIGHT_46_TM=61.129
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=5.1000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=517
PRIMER_PAIR_47_PENALTY=1.174150
PRIMER_LEFT_47_PENALTY=0.018700
PRIMER_RIGHT_47_PENALTY=1.155450
PRIMER_LEFT_47_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_47_SEQUENCE=CATCGGGTTCTTGTGAATTGT
PRIMER_LEFT_47=773,20
PRIMER_RIGHT_47=1498,21
PRIMER_LEFT_47_TM=60.019
PRIMER_RIGHT_47_TM=59.845
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=5.1000
PRIMER_RIGHT_47_END_STABILITY=6.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=726
PRIMER_PAIR_48_PENALTY=1.180335
PRIMER_LEFT_48_PENALTY=0.018700
PRIMER_RIGHT_48_PENALTY=1.161636
PRIMER_LEFT_48_SEQUENCE=GTCGAGCATCGCAACTACAA
PRIMER_RIGHT_48_SEQUENCE=CACAATGCAACCATTCATCAC
PRIMER_LEFT_48=775,20
PRIMER_RIGHT_48=1282,21
PRIMER_LEFT_48_TM=60.019
PRIMER_RIGHT_48_TM=59.838
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.0000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=508
PRIMER_PAIR_49_PENALTY=1.180335
PRIMER_LEFT_49_PENALTY=0.018700
PRIMER_RIGHT_49_PENALTY=1.161636
PRIMER_LEFT_49_SEQUENCE=TTGTCGAGCATCGCAACTAC
PRIMER_RIGHT_49_SEQUENCE=CACAATGCAACCATTCATCAC
PRIMER_LEFT_49=773,20
PRIMER_RIGHT_49=1282,21
PRIMER_LEFT_49_TM=60.019
PRIMER_RIGHT_49_TM=59.838
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.1000
PRIMER_RIGHT_49_END_STABILITY=6.3000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=510
=
SEQUENCE_ID=MAGIC.446_4_4127594_4127968_oy-0_wu-0_17673_239622_TRUE
SEQUENCE_TARGET=950,474
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGNAAGANTTGNTNGNNCGGGTAAAGTTTATANTCTTAATCATGTAGAGCGGCAACAAGCANATCGACATGTATTGGTCAACTGCNNACTACTAGATCACCTNCGAGAGTAAGTTATCTAATTCAAGAACAACTAATTATATTTATGTTTTATCGAAATTCTCTAATGTAGACNTTATGGTTGTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATTNNCATGTTTCCCAANGGACGCACGTAACGGCACGTTGAATTTTTTCACACNNNNNACACATGCATGTAAACCGGTTTGAACNAGTCTTAACCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_24175133_24175755_no-0_mt-0_519555_2085_TRUE
SEQUENCE_TARGET=950,722
SEQUENCE_TEMPLATE=GTCTTAGTTGCCTGAAGCTTTAGNTTATTTCTTTCTTCCATAAGGGACTGAAAGAGTGAGCCTTTCCGCTCTACATCCTGCTCNGGATTCAATTTCTCCGTCCACTTATGCGCATTCTCTAATATTCTTTTATTGAAACCAATGGACTTAGCTANTCTCAATGCATTTGATAGACCAGTACTTCCCCAGAGTACTCGAAATGTAGGCTGCAAAGTTTCCATGGAGAACTCCATTGCAGCATTTTGGAATCGAGGNTCATTATNCTTCAANCGACTTAAGTCACCGTAATGTGTTGATACAACNGCCACATTAACACGGTTNTTAATATACTGTAGGATNCTAGTAGCAAGTGCAACCCCTTCTGAAGGATCAGTTCCACTACATATTTCGTCTAGTAANACAAGNGAATTTTCTGAAGCAATGTCAANTATCTGNCGTATCCGTGAGATGTGNCCACTAAAGGTTGANAGACTTTGCTCCAAAGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNTTGCAAGGAATTCANAANGTAATACCTGNGGATCNCCNATNTCAGCCAGAATAAGATCAAACCAAGGCAACCTTGGGCAGTTCTTAGCAGGCAAATACATNCCGGACTTNGACATAAGAGACAATAATCCCAATGTCTTTAANAAAGCNGTCTTNCCTCCTGTGTTTGGACCCGAGATGACGACTACTTTGGCACTGCTTTCAACTTTAATNTCTACTGGCACAGGGAAAATGTCTCCACCNTTTGGNCTGCCTAATACTGAACCAAGAAGTAGCGGGTGCTGCGCAGAATCAATGTCTACAGCTAAGCCCNGTGTTTTNGTGTGTTCTGAAGTTACGTTGGGATAGACACCATTAATCCANNTTGCATGTGATGCTCTTGCAAAAGCAATGTCAAGTTCTAATATTCTATCCAACAAATNCAATATNTCNCTCTGCGCCATNACCACCTCNGATGTCAAAATGCNCAAAATCGCCNTTTCCTCAGCTTTCTCNNAGTTNGCATGTCTCACTTCCATATTATTAAGCTCCACTGCCTCTTTCGGTTCTATNAANCATGTTGCCCTNGAGCTACTAACACTTAAAACAACACCACCTGGAAGTAAACTTTTGTGTGTNGCTCTAANAGCTACACACATCCTTGATCTCCTCTGCGTTATCAAAGGCTTGTTAATCCCCCCAGCTAGAAAAATCTTNGTNGATATTTTCTTCAAAAGAGAATCCAAANTCTCCATGTTTCTNCTCCGTTCAGATCTTATAATCTCTAAATCTTCACTAGCTCTATCAAGAATCATAGTCATNTTGCAGTCNATACAAAAACTAATCTTCTGCTGTAATGTATCCTTGAAATCACAACCCTGTAGTATATCTACAAGTGGAGTAACCCTACAAAATAANAGAAGNAANAGCTAGAACCATNAGCNTCTCAATAGCAATANGAACANCTAATAACTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNTNNCNNNNNNNNTNNNNNGNNNNNNNANNNNCNNNNNNCNNNNNNNNNANNNTNCNNNNNTNTCNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNCGNNNNNNNNNNNNNNNNNNNNNNGNNNNTNNNGNNNNNNNNNNGNNNNNGAGNNNNCNNNNNNNNNNNGGCNNNNNNNNNNNNNGANNNNNNNNNNNNNNNGTNNNNGNNNNNCNNNNNNNNGNNNNNGNNNNNGNNNNNNNNNNANNNNANNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNGNCNGNNNGNGNNNNGNNNNNNGNNGCGNCGCGNNNNNNNNNNNNNGNNNNNNNNCGNNNNNANNNNNNNNNGGNNNNNNGTGNNNANTTGNGNTGAATNNGNNTNNNNATNNNTTNNNNNNNNNNANNAGTNTTNANNNCTGTGGNNAANGANNNNTCNANNNTNCNNTTNNNNTNTGNNNNNNNTCNGNNNNTNNNNAGTTNNNNNNNNTTNNNTNNGGANNNNNNNNTTGNNCNNNTTAANNNANNTTGTTCNNGNTTNAANNNNTNNNNNANTNNNNTTCGNTNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNTNNNNNNNNNTNNNNNANNNNNNNNNNNNNTNNNNANCNANNNNNNNNNNNTNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNTNNNNAGNNNNNNNNNNNNNNTNNNNNTNNNNNTNTNNGNNNNANNNNNNNGNNNNNNNCNNNNNTNNNNGNNNNNNNNNNNTNNNNNCNCNNCNNCNCCNNTNNTCNNCNNTNNNCTNCTCNCNNNNNCNCCCNCNNCNTNNNNNNCTCNNNNTNNNNCTCNNNTNCNCNNNCNCNCNNNTNNCCNNNNNNNNCNNNNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_22596642_22597148_sf-2_rsch-4_23676_244644_TRUE
SEQUENCE_TARGET=950,606
SEQUENCE_TEMPLATE=ACTGAAGTCTGAAACTNGTGTTTTCTTGTACTGTNTGTGACATGCATNACTTNANCANAGNCATAATGTNNAAAAANNNAAATTANNTATTTGGAACAAGGAANATGAACGTAAACAAATNTAAGNTTTGGANAAAANTTGTTCTAGAAAGGCTGTAGNTACNAAATCAAGTTTCNNCAGTACNAGGATGATGTTAGNGGAAATTAATATAGATTTACACCAAATTNATTGAATNATATTAAATAAATCAATANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGTAGTANCACTNATTTTTNATACAANTTNAAATAAGTGGCTGCAACCNNNNNNNNNNNNNNNNNTAATCAGTCATACCAACTAAAAATTTAATAAAAGNAANTNCAACATTCATTNCNATANCTTCACCNTCATAGAANGTAGATTCTGAAATCAAGTAGCNGCCACTTGACCATCATAGGAAACAACNTTGAAAACGTAGATTCTGAATTCANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNTAAGGGTGGTATTCTTACGTAAGTTAATGAATTTGANTTANNNATGGNCANATATAACCNGATTAGTCTNGAGACCAATAAANTGGTATGTATNAATGCATGAGTNNNNNNNGTGGGGNAAAGCATNTATACTTACTAAATACANTATAAAANTAAACCAACGCATTTTTNNNTGAAGAGACAANAGTCTTGTCGNGNAAAANGTNCAACTATAGCTTCTGCANTAAGTATNTATNTNAAATATTCATANTTTGACCANNNNNTTTTTTANGTCTTTTAAATGGNGTCGTCGGACTANGATTTNNNNNNTNTNNGANTNNNNANNNNNNNATNNNANNNAAANNCCCNNNGNGTNNTNTCNTGNNTTNTNANNNTNCAAANNATNGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGANNNNTTNTNNNNNNNNNNNATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAAANTACTATATTGTATGNAACNTAACGGTNATATATATTGGACCGTTATAAGANGAAAGGTNGTGANCCCAATTTGGGTNAGCACTTAGCAGCTGCAGCCCCACTGCTCGTGNNNNNNNNNNNTCCAGCTCCAAAGTTNANTTCGCNAGTGGNGCNACAAAACTTTNCCANTTTTGTTTCTGCTATTTATTTACNNNNATACGNCAGTTTTCTATAAATTNNAGNACAAGTTGATGNATNANANTTTTNTNNTGTGTGCNTTTTANNCNGTTANTTGATNAACGTNGAAAANTCTGANTTNNGNTNTTNTTNTGAGTGNATTATTTTCACTCNGCATGGNNNNNNNNNNNNNNNNNNNACGGCATCATGATCTGNTNAATTGAAGAAAATNAATTAAANCAAGTNGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTANNATTAGNCANCAAAATTNNAGNAATNTNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATATANAGACAGNTTGTGGCGNACNNCAACNATAAAAACGAAACGATANNTGTCNNNGCANTCATCTATTAAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_10020332_10020404_hi-0_zu-0_1570052_20947_TRUE
SEQUENCE_TARGET=950,172
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCATANANANAAAATCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGGCCACTAGCTCTAACCAGATTCAAGTTAATCAAAATNCTTGTGTGGANACNANACTAGTGAAAGANATATGGCAGAGACTANAAAGGAGATGCNAAAATCTTCTCTATGGGGAGAGGTTGAGGTAGAAGTTGACATCAAATCTCCTGCTGAAAAATTCTACCANGTGTATGTAGGAAGACCAGACCATGTCGCTAAAGCCACTTCTAGCAAAGTGCAAGCATGTGATCTGCTTGAAGGNGAATGGGGCACCATANGCAGTATTGTCAANTGGAACTACGTTTATGGTAACAAATTATNAATTTATANNTCTTTAANNTATCGTAAATNTTTGGTGATTTTATTNGTGAATGTTTTGAGTGTAGCGGGCAAGGCAAAGGTGGCAAAAGAGAGAATCGAATTAGTNGAACCNGAGAAGAAACTAATAAAGTTTAGGGTCATAGANGGAGATGTTTTGGNTGTGTACAAGAACTTCTTNATCACGATTNNAGTGACCNCGAAGGANGGAGGGGTTGGAAGTGTGGCNAAATGGCACCTTGAGTACGAGAAGAATGATGNGAATGTGCCTGACCCCGAGAATTTNNTCCCTTTCTTAGCTGAGATGACNAAAGAGATNGATGAACACCTCNTATCCGAGGAATAGACATACTCTNAGTNTTGTTGATATCTATTTCTCTTGCTCTGGCTAGATTNAAGATGAAATAAAGTTTGTGTTGATTGGTTAATGATATNTCATGGTGTTTGGATATTTGATGTTNAGTTTNNTTTTGTAAGTTGCAATTTGTAACNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17184537_17184769_col-0_kn-0_16637_99802_TRUE
SEQUENCE_TARGET=950,332
SEQUENCE_TEMPLATE=CATATTNCTNTTTATNTTGGNGNAGAAACTAAATATGTTACCACCACTCTTTTTATAAAGTATACTTTTTGATAATTCTAAACAATANACTNNTATACTAAACACAACAATTCATCAAATCCATGTAAACGCATATACTATTTCATCTATGTGTTGNAGGCTGGTGCTTTAGGGTCNTACCTTCNNTACCACCCATCTTGGTTCTGTGTGAACGAAGANGAAACTCTTTGGCACGTGGGNAAAAGCTTCGGTGCACGTGTCTNGTATGAGTTTGTTCTTTTCTTAANCACACACANACAACACCGTTCGTTTCCTTCGATTNTCATTGGNTTAGAACTCTCGAGAAACCAGATGANTNCTCACTCCGTTAATCGGACTCCGTTACGCTGAGTTGTTATTTTTCTTATTCTATTTATATCTTTTGTTTGATTACATTATGGGAGTGCATTTTATATTCAAGGAAAAAGGCAATTCTCACACGCCTCTCAGCGAATTAAGATTACGTCTGTCGGAGTTTCGATCTTTCGCTTGTTCCCAAGACAAATAAAGTTAGTTTTCATTCTTGTGCTAGCTATATCGACCTATGTGTAACGCTAGATCTANATTCNGCATATCTTGAGAAGCATCAGATTTTCTTATGNATATGGCAANTTTTNCCAAAATCCATCAACTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANAGAGAGAATTTGATCATATTTANAANAAAAAAAAAACTCTTTAAATTGNAAGCTGAACGTCACGCNCTCACGGGTCTGGACGTAATCAGAATACAAGATTCACTGACGCCAAANNNNNNGTAACTGTAACTTTTTCTTACCANTTGGTAAAACTAAAAAGGAATCTCGGTCGGATCCTGAGGCTTGATTTAGCGCCACGTTAGTCGTCAGTTANCTTTCGCGCCTTTGACTCTTCGTAGCGATCCTTTTCATGTCGCTTTTTTTCCTTTGTAACTTTTTTTGTTTTTGCTTTAAACATTTTTGTTTCTTTTTTACAACTTTTTCCATTAAAGAAAAAAANCTTNAACCAAACCCTTTACAACAATACAACATTATGGAAGTACAGGAGTTTATAATCATGTNTTATAAACATAATTATTGCTTACCAAATGAAATGAGACATGTCNATCAAATTACAATTTCTAATTGCCGTTATCAAAGGATCNACNTNTTTTNGTTATAAGCTTACCGTTGAGCTCATCATCTCATCAAATGATTAATTTCTGTTGACTGTTGATAAACTTTTACAACGAAACTGTCGACTGAATACCTTTTCGATNTCGCATTAACTGTATATATCTTCGTTATTAATACTATTTTNACAATTTTTACGGACCACTAGCTAGTACATGCAAATAAGGAAACTGATCTGTATANTTGATGGAGATCTTTTTGTATNAAAATTACAATGTTACCACACCTTACTTCGTAAATAAGAATTCATCATCTAGTACTTCACATGTTCGATGGTTGAGTCAATCATAAATAATATAGATNCCCACACCAAAGGAGTCATTTGCGTTTCAAGGAAAACATTACATTAAATGTTGATAGTGCCATGACTGTATTACCTCAAATATGGGGATGCAAAAATATCTACTATACAGAAATGTTTCTGTAACCTAATCGAAACTAAACTATATTTATAATATGATTATATGATCGTACCGATTTGAACTAATTGAACTATATATGAATACATAANANCATAACNAAAAAAGGNATTTGTAGAAAGTATAAAGAAAAAATTAAGCTTANNNAAAACTTAAATTGTATTTAATTAGTATCAAACTAAATCAAATAGCGACGCCTGCAACTTAATTTATCAATNCCNNGCACAAAACATTCTTTCNGGATAATAGAACANAATTAATGATATGCTATANTAACATCTATAANTTGGTTCAATNTTTGTTAGACAAAGGCTTCGCATGAAATGAATATAATAACGGTCATTAACGAGACTTATGTTAATGACCTTCATGGAGTCATTATTAAAGCGTACATAATNTTAAAGAATTATTGCAAACCATTATATTGTTTTCTTTCTCCCATGATCCGTGAACAAACATAACATAAATATTTAATATTATCTCTAGAATTTGTTGTTGGTCATGAAAGAAAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.190220
PRIMER_LEFT_0_PENALTY=0.010545
PRIMER_RIGHT_0_PENALTY=0.179674
PRIMER_LEFT_0_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_0_SEQUENCE=AATGACTCCTTTGGTGTGGG
PRIMER_LEFT_0=856,20
PRIMER_RIGHT_0=1622,20
PRIMER_LEFT_0_TM=59.989
PRIMER_RIGHT_0_TM=59.820
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=767
PRIMER_PAIR_1_PENALTY=1.103459
PRIMER_LEFT_1_PENALTY=0.923785
PRIMER_RIGHT_1_PENALTY=0.179674
PRIMER_LEFT_1_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_1_SEQUENCE=AATGACTCCTTTGGTGTGGG
PRIMER_LEFT_1=854,20
PRIMER_RIGHT_1=1622,20
PRIMER_LEFT_1_TM=60.924
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=769
PRIMER_PAIR_2_PENALTY=1.263292
PRIMER_LEFT_2_PENALTY=0.010545
PRIMER_RIGHT_2_PENALTY=1.252746
PRIMER_LEFT_2_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_2_SEQUENCE=TTCCTTGAAACGCAAATGAC
PRIMER_LEFT_2=856,20
PRIMER_RIGHT_2=1636,20
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=58.747
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=781
PRIMER_PAIR_3_PENALTY=1.412496
PRIMER_LEFT_3_PENALTY=0.159750
PRIMER_RIGHT_3_PENALTY=1.252746
PRIMER_LEFT_3_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_3_SEQUENCE=TTCCTTGAAACGCAAATGAC
PRIMER_LEFT_3=880,20
PRIMER_RIGHT_3=1636,20
PRIMER_LEFT_3_TM=59.840
PRIMER_RIGHT_3_TM=58.747
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=757
PRIMER_PAIR_4_PENALTY=1.454294
PRIMER_LEFT_4_PENALTY=0.010545
PRIMER_RIGHT_4_PENALTY=1.443748
PRIMER_LEFT_4_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_4_SEQUENCE=CTCAACCATCGAACATGTGAA
PRIMER_LEFT_4=856,20
PRIMER_RIGHT_4=1580,21
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=59.556
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=725
PRIMER_PAIR_5_PENALTY=1.553722
PRIMER_LEFT_5_PENALTY=0.010545
PRIMER_RIGHT_5_PENALTY=1.543176
PRIMER_LEFT_5_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_5_SEQUENCE=TCAACCATCGAACATGTGAA
PRIMER_LEFT_5=856,20
PRIMER_RIGHT_5=1579,20
PRIMER_LEFT_5_TM=59.989
PRIMER_RIGHT_5_TM=58.457
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=724
PRIMER_PAIR_6_PENALTY=1.603498
PRIMER_LEFT_6_PENALTY=0.159750
PRIMER_RIGHT_6_PENALTY=1.443748
PRIMER_LEFT_6_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_6_SEQUENCE=CTCAACCATCGAACATGTGAA
PRIMER_LEFT_6=880,20
PRIMER_RIGHT_6=1580,21
PRIMER_LEFT_6_TM=59.840
PRIMER_RIGHT_6_TM=59.556
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.8000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=701
PRIMER_PAIR_7_PENALTY=1.658319
PRIMER_LEFT_7_PENALTY=0.010545
PRIMER_RIGHT_7_PENALTY=1.647773
PRIMER_LEFT_7_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_7_SEQUENCE=CTTGAAACGCAAATGACTCC
PRIMER_LEFT_7=856,20
PRIMER_RIGHT_7=1633,20
PRIMER_LEFT_7_TM=59.989
PRIMER_RIGHT_7_TM=58.352
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=778
PRIMER_PAIR_8_PENALTY=1.658319
PRIMER_LEFT_8_PENALTY=0.010545
PRIMER_RIGHT_8_PENALTY=1.647773
PRIMER_LEFT_8_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_8_SEQUENCE=TCCTTGAAACGCAAATGACT
PRIMER_LEFT_8=856,20
PRIMER_RIGHT_8=1635,20
PRIMER_LEFT_8_TM=59.989
PRIMER_RIGHT_8_TM=58.352
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=780
PRIMER_PAIR_9_PENALTY=1.702926
PRIMER_LEFT_9_PENALTY=0.159750
PRIMER_RIGHT_9_PENALTY=1.543176
PRIMER_LEFT_9_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_9_SEQUENCE=TCAACCATCGAACATGTGAA
PRIMER_LEFT_9=880,20
PRIMER_RIGHT_9=1579,20
PRIMER_LEFT_9_TM=59.840
PRIMER_RIGHT_9_TM=58.457
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.8000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=700
PRIMER_PAIR_10_PENALTY=1.807523
PRIMER_LEFT_10_PENALTY=0.159750
PRIMER_RIGHT_10_PENALTY=1.647773
PRIMER_LEFT_10_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_10_SEQUENCE=CTTGAAACGCAAATGACTCC
PRIMER_LEFT_10=880,20
PRIMER_RIGHT_10=1633,20
PRIMER_LEFT_10_TM=59.840
PRIMER_RIGHT_10_TM=58.352
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.8000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=754
PRIMER_PAIR_11_PENALTY=1.807523
PRIMER_LEFT_11_PENALTY=0.159750
PRIMER_RIGHT_11_PENALTY=1.647773
PRIMER_LEFT_11_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_11_SEQUENCE=TCCTTGAAACGCAAATGACT
PRIMER_LEFT_11=880,20
PRIMER_RIGHT_11=1635,20
PRIMER_LEFT_11_TM=59.840
PRIMER_RIGHT_11_TM=58.352
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.8000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=756
PRIMER_PAIR_12_PENALTY=2.176531
PRIMER_LEFT_12_PENALTY=0.923785
PRIMER_RIGHT_12_PENALTY=1.252746
PRIMER_LEFT_12_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_12_SEQUENCE=TTCCTTGAAACGCAAATGAC
PRIMER_LEFT_12=854,20
PRIMER_RIGHT_12=1636,20
PRIMER_LEFT_12_TM=60.924
PRIMER_RIGHT_12_TM=58.747
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=6.3000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=783
PRIMER_PAIR_13_PENALTY=2.367533
PRIMER_LEFT_13_PENALTY=0.923785
PRIMER_RIGHT_13_PENALTY=1.443748
PRIMER_LEFT_13_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_13_SEQUENCE=CTCAACCATCGAACATGTGAA
PRIMER_LEFT_13=854,20
PRIMER_RIGHT_13=1580,21
PRIMER_LEFT_13_TM=60.924
PRIMER_RIGHT_13_TM=59.556
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=5.9000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=727
PRIMER_PAIR_14_PENALTY=2.429231
PRIMER_LEFT_14_PENALTY=0.010545
PRIMER_RIGHT_14_PENALTY=2.418685
PRIMER_LEFT_14_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_14_SEQUENCE=TCAACCATCGAACATGTGAAGT
PRIMER_LEFT_14=856,20
PRIMER_RIGHT_14=1579,22
PRIMER_LEFT_14_TM=59.989
PRIMER_RIGHT_14_TM=60.419
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=724
PRIMER_PAIR_15_PENALTY=2.429231
PRIMER_LEFT_15_PENALTY=0.010545
PRIMER_RIGHT_15_PENALTY=2.418685
PRIMER_LEFT_15_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_15_SEQUENCE=ACTCAACCATCGAACATGTGAA
PRIMER_LEFT_15=856,20
PRIMER_RIGHT_15=1581,22
PRIMER_LEFT_15_TM=59.989
PRIMER_RIGHT_15_TM=60.419
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=726
PRIMER_PAIR_16_PENALTY=2.466961
PRIMER_LEFT_16_PENALTY=0.923785
PRIMER_RIGHT_16_PENALTY=1.543176
PRIMER_LEFT_16_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_16_SEQUENCE=TCAACCATCGAACATGTGAA
PRIMER_LEFT_16=854,20
PRIMER_RIGHT_16=1579,20
PRIMER_LEFT_16_TM=60.924
PRIMER_RIGHT_16_TM=58.457
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.9000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=726
PRIMER_PAIR_17_PENALTY=2.526848
PRIMER_LEFT_17_PENALTY=0.010545
PRIMER_RIGHT_17_PENALTY=2.516303
PRIMER_LEFT_17_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_17_SEQUENCE=CAACCATCGAACATGTGAAGT
PRIMER_LEFT_17=856,20
PRIMER_RIGHT_17=1578,21
PRIMER_LEFT_17_TM=59.989
PRIMER_RIGHT_17_TM=58.484
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=723
PRIMER_PAIR_18_PENALTY=2.571558
PRIMER_LEFT_18_PENALTY=0.923785
PRIMER_RIGHT_18_PENALTY=1.647773
PRIMER_LEFT_18_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_18_SEQUENCE=CTTGAAACGCAAATGACTCC
PRIMER_LEFT_18=854,20
PRIMER_RIGHT_18=1633,20
PRIMER_LEFT_18_TM=60.924
PRIMER_RIGHT_18_TM=58.352
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=5.9000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=780
PRIMER_PAIR_19_PENALTY=2.571558
PRIMER_LEFT_19_PENALTY=0.923785
PRIMER_RIGHT_19_PENALTY=1.647773
PRIMER_LEFT_19_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_19_SEQUENCE=TCCTTGAAACGCAAATGACT
PRIMER_LEFT_19=854,20
PRIMER_RIGHT_19=1635,20
PRIMER_LEFT_19_TM=60.924
PRIMER_RIGHT_19_TM=58.352
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=782
PRIMER_PAIR_20_PENALTY=2.578435
PRIMER_LEFT_20_PENALTY=0.159750
PRIMER_RIGHT_20_PENALTY=2.418685
PRIMER_LEFT_20_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_20_SEQUENCE=TCAACCATCGAACATGTGAAGT
PRIMER_LEFT_20=880,20
PRIMER_RIGHT_20=1579,22
PRIMER_LEFT_20_TM=59.840
PRIMER_RIGHT_20_TM=60.419
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.8000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=700
PRIMER_PAIR_21_PENALTY=2.578435
PRIMER_LEFT_21_PENALTY=0.159750
PRIMER_RIGHT_21_PENALTY=2.418685
PRIMER_LEFT_21_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_21_SEQUENCE=ACTCAACCATCGAACATGTGAA
PRIMER_LEFT_21=880,20
PRIMER_RIGHT_21=1581,22
PRIMER_LEFT_21_TM=59.840
PRIMER_RIGHT_21_TM=60.419
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.8000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=702
PRIMER_PAIR_22_PENALTY=2.599193
PRIMER_LEFT_22_PENALTY=0.010545
PRIMER_RIGHT_22_PENALTY=2.588648
PRIMER_LEFT_22_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_22_SEQUENCE=TTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_22=856,20
PRIMER_RIGHT_22=1372,21
PRIMER_LEFT_22_TM=59.989
PRIMER_RIGHT_22_TM=58.411
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=517
PRIMER_PAIR_23_PENALTY=2.599872
PRIMER_LEFT_23_PENALTY=2.420198
PRIMER_RIGHT_23_PENALTY=0.179674
PRIMER_LEFT_23_SEQUENCE=GGGTCTGGACGTAATCAGAA
PRIMER_RIGHT_23_SEQUENCE=AATGACTCCTTTGGTGTGGG
PRIMER_LEFT_23=858,20
PRIMER_RIGHT_23=1622,20
PRIMER_LEFT_23_TM=57.580
PRIMER_RIGHT_23_TM=59.820
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=765
PRIMER_PAIR_24_PENALTY=2.676053
PRIMER_LEFT_24_PENALTY=0.159750
PRIMER_RIGHT_24_PENALTY=2.516303
PRIMER_LEFT_24_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_24_SEQUENCE=CAACCATCGAACATGTGAAGT
PRIMER_LEFT_24=880,20
PRIMER_RIGHT_24=1578,21
PRIMER_LEFT_24_TM=59.840
PRIMER_RIGHT_24_TM=58.484
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=699
PRIMER_PAIR_25_PENALTY=2.748398
PRIMER_LEFT_25_PENALTY=0.159750
PRIMER_RIGHT_25_PENALTY=2.588648
PRIMER_LEFT_25_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_25_SEQUENCE=TTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_25=880,20
PRIMER_RIGHT_25=1372,21
PRIMER_LEFT_25_TM=59.840
PRIMER_RIGHT_25_TM=58.411
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.8000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=493
PRIMER_PAIR_26_PENALTY=2.903972
PRIMER_LEFT_26_PENALTY=0.010545
PRIMER_RIGHT_26_PENALTY=2.893427
PRIMER_LEFT_26_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_26_SEQUENCE=CCTTGAAACGCAAATGACTCC
PRIMER_LEFT_26=856,20
PRIMER_RIGHT_26=1634,21
PRIMER_LEFT_26_TM=59.989
PRIMER_RIGHT_26_TM=61.893
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=779
PRIMER_PAIR_27_PENALTY=2.919513
PRIMER_LEFT_27_PENALTY=0.330865
PRIMER_RIGHT_27_PENALTY=2.588648
PRIMER_LEFT_27_SEQUENCE=TTCGATCTTTCGCTTGTTCC
PRIMER_RIGHT_27_SEQUENCE=TTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_27=515,20
PRIMER_RIGHT_27=1372,21
PRIMER_LEFT_27_TM=60.331
PRIMER_RIGHT_27_TM=58.411
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=858
PRIMER_PAIR_28_PENALTY=2.936341
PRIMER_LEFT_28_PENALTY=1.683594
PRIMER_RIGHT_28_PENALTY=1.252746
PRIMER_LEFT_28_SEQUENCE=ACAAGATTCACTGACGCCAAA
PRIMER_RIGHT_28_SEQUENCE=TTCCTTGAAACGCAAATGAC
PRIMER_LEFT_28=879,21
PRIMER_RIGHT_28=1636,20
PRIMER_LEFT_28_TM=60.684
PRIMER_RIGHT_28_TM=58.747
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.8000
PRIMER_RIGHT_28_END_STABILITY=6.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=758
PRIMER_PAIR_29_PENALTY=3.021878
PRIMER_LEFT_29_PENALTY=2.842204
PRIMER_RIGHT_29_PENALTY=0.179674
PRIMER_LEFT_29_SEQUENCE=CTCACGGGTCTGGACGTAATC
PRIMER_RIGHT_29_SEQUENCE=AATGACTCCTTTGGTGTGGG
PRIMER_LEFT_29=853,21
PRIMER_RIGHT_29=1622,20
PRIMER_LEFT_29_TM=61.842
PRIMER_RIGHT_29_TM=59.820
PRIMER_LEFT_29_GC_PERCENT=57.143
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=5.9000
PRIMER_RIGHT_29_END_STABILITY=9.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=770
PRIMER_PAIR_30_PENALTY=3.053177
PRIMER_LEFT_30_PENALTY=0.159750
PRIMER_RIGHT_30_PENALTY=2.893427
PRIMER_LEFT_30_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_30_SEQUENCE=CCTTGAAACGCAAATGACTCC
PRIMER_LEFT_30=880,20
PRIMER_RIGHT_30=1634,21
PRIMER_LEFT_30_TM=59.840
PRIMER_RIGHT_30_TM=61.893
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.8000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=755
PRIMER_PAIR_31_PENALTY=3.127343
PRIMER_LEFT_31_PENALTY=1.683594
PRIMER_RIGHT_31_PENALTY=1.443748
PRIMER_LEFT_31_SEQUENCE=ACAAGATTCACTGACGCCAAA
PRIMER_RIGHT_31_SEQUENCE=CTCAACCATCGAACATGTGAA
PRIMER_LEFT_31=879,21
PRIMER_RIGHT_31=1580,21
PRIMER_LEFT_31_TM=60.684
PRIMER_RIGHT_31_TM=59.556
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.8000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=702
PRIMER_PAIR_32_PENALTY=3.178007
PRIMER_LEFT_32_PENALTY=0.010545
PRIMER_RIGHT_32_PENALTY=3.167461
PRIMER_LEFT_32_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_32_SEQUENCE=TCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_32=856,20
PRIMER_RIGHT_32=1371,20
PRIMER_LEFT_32_TM=59.989
PRIMER_RIGHT_32_TM=56.833
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=516
PRIMER_PAIR_33_PENALTY=3.205737
PRIMER_LEFT_33_PENALTY=0.617089
PRIMER_RIGHT_33_PENALTY=2.588648
PRIMER_LEFT_33_SEQUENCE=CGCCTCTCAGCGAATTAAGA
PRIMER_RIGHT_33_SEQUENCE=TTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_33=479,20
PRIMER_RIGHT_33=1372,21
PRIMER_LEFT_33_TM=60.617
PRIMER_RIGHT_33_TM=58.411
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.0000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=894
PRIMER_PAIR_34_PENALTY=3.226771
PRIMER_LEFT_34_PENALTY=1.683594
PRIMER_RIGHT_34_PENALTY=1.543176
PRIMER_LEFT_34_SEQUENCE=ACAAGATTCACTGACGCCAAA
PRIMER_RIGHT_34_SEQUENCE=TCAACCATCGAACATGTGAA
PRIMER_LEFT_34=879,21
PRIMER_RIGHT_34=1579,20
PRIMER_LEFT_34_TM=60.684
PRIMER_RIGHT_34_TM=58.457
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=701
PRIMER_PAIR_35_PENALTY=3.239329
PRIMER_LEFT_35_PENALTY=0.010545
PRIMER_RIGHT_35_PENALTY=3.228784
PRIMER_LEFT_35_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_35_SEQUENCE=ATTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_35=856,20
PRIMER_RIGHT_35=1373,22
PRIMER_LEFT_35_TM=59.989
PRIMER_RIGHT_35_TM=58.771
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=518
PRIMER_PAIR_36_PENALTY=3.327211
PRIMER_LEFT_36_PENALTY=0.159750
PRIMER_RIGHT_36_PENALTY=3.167461
PRIMER_LEFT_36_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_36_SEQUENCE=TCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_36=880,20
PRIMER_RIGHT_36=1371,20
PRIMER_LEFT_36_TM=59.840
PRIMER_RIGHT_36_TM=56.833
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.8000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=492
PRIMER_PAIR_37_PENALTY=3.331367
PRIMER_LEFT_37_PENALTY=1.683594
PRIMER_RIGHT_37_PENALTY=1.647773
PRIMER_LEFT_37_SEQUENCE=ACAAGATTCACTGACGCCAAA
PRIMER_RIGHT_37_SEQUENCE=CTTGAAACGCAAATGACTCC
PRIMER_LEFT_37=879,21
PRIMER_RIGHT_37=1633,20
PRIMER_LEFT_37_TM=60.684
PRIMER_RIGHT_37_TM=58.352
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.8000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=755
PRIMER_PAIR_38_PENALTY=3.331367
PRIMER_LEFT_38_PENALTY=1.683594
PRIMER_RIGHT_38_PENALTY=1.647773
PRIMER_LEFT_38_SEQUENCE=ACAAGATTCACTGACGCCAAA
PRIMER_RIGHT_38_SEQUENCE=TCCTTGAAACGCAAATGACT
PRIMER_LEFT_38=879,21
PRIMER_RIGHT_38=1635,20
PRIMER_LEFT_38_TM=60.684
PRIMER_RIGHT_38_TM=58.352
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.8000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=757
PRIMER_PAIR_39_PENALTY=3.342470
PRIMER_LEFT_39_PENALTY=0.923785
PRIMER_RIGHT_39_PENALTY=2.418685
PRIMER_LEFT_39_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_39_SEQUENCE=TCAACCATCGAACATGTGAAGT
PRIMER_LEFT_39=854,20
PRIMER_RIGHT_39=1579,22
PRIMER_LEFT_39_TM=60.924
PRIMER_RIGHT_39_TM=60.419
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.9000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=726
PRIMER_PAIR_40_PENALTY=3.342470
PRIMER_LEFT_40_PENALTY=0.923785
PRIMER_RIGHT_40_PENALTY=2.418685
PRIMER_LEFT_40_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_40_SEQUENCE=ACTCAACCATCGAACATGTGAA
PRIMER_LEFT_40=854,20
PRIMER_RIGHT_40=1581,22
PRIMER_LEFT_40_TM=60.924
PRIMER_RIGHT_40_TM=60.419
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.9000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=728
PRIMER_PAIR_41_PENALTY=3.388533
PRIMER_LEFT_41_PENALTY=0.159750
PRIMER_RIGHT_41_PENALTY=3.228784
PRIMER_LEFT_41_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_41_SEQUENCE=ATTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_41=880,20
PRIMER_RIGHT_41=1373,22
PRIMER_LEFT_41_TM=59.840
PRIMER_RIGHT_41_TM=58.771
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.8000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=494
PRIMER_PAIR_42_PENALTY=3.432464
PRIMER_LEFT_42_PENALTY=0.010545
PRIMER_RIGHT_42_PENALTY=3.421919
PRIMER_LEFT_42_SEQUENCE=ACGGGTCTGGACGTAATCAG
PRIMER_RIGHT_42_SEQUENCE=GGTATTCAGTCGACAGTTTCGTT
PRIMER_LEFT_42=856,20
PRIMER_RIGHT_42=1376,23
PRIMER_LEFT_42_TM=59.989
PRIMER_RIGHT_42_TM=59.578
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=521
PRIMER_PAIR_43_PENALTY=3.440088
PRIMER_LEFT_43_PENALTY=0.923785
PRIMER_RIGHT_43_PENALTY=2.516303
PRIMER_LEFT_43_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_43_SEQUENCE=CAACCATCGAACATGTGAAGT
PRIMER_LEFT_43=854,20
PRIMER_RIGHT_43=1578,21
PRIMER_LEFT_43_TM=60.924
PRIMER_RIGHT_43_TM=58.484
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=5.9000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=725
PRIMER_PAIR_44_PENALTY=3.496279
PRIMER_LEFT_44_PENALTY=3.316605
PRIMER_RIGHT_44_PENALTY=0.179674
PRIMER_LEFT_44_SEQUENCE=CGGGTCTGGACGTAATCAGAA
PRIMER_RIGHT_44_SEQUENCE=AATGACTCCTTTGGTGTGGG
PRIMER_LEFT_44=857,21
PRIMER_RIGHT_44=1622,20
PRIMER_LEFT_44_TM=62.317
PRIMER_RIGHT_44_TM=59.820
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=766
PRIMER_PAIR_45_PENALTY=3.498326
PRIMER_LEFT_45_PENALTY=0.330865
PRIMER_RIGHT_45_PENALTY=3.167461
PRIMER_LEFT_45_SEQUENCE=TTCGATCTTTCGCTTGTTCC
PRIMER_RIGHT_45_SEQUENCE=TCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_45=515,20
PRIMER_RIGHT_45=1371,20
PRIMER_LEFT_45_TM=60.331
PRIMER_RIGHT_45_TM=56.833
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=857
PRIMER_PAIR_46_PENALTY=3.512433
PRIMER_LEFT_46_PENALTY=0.923785
PRIMER_RIGHT_46_PENALTY=2.588648
PRIMER_LEFT_46_SEQUENCE=TCACGGGTCTGGACGTAATC
PRIMER_RIGHT_46_SEQUENCE=TTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_46=854,20
PRIMER_RIGHT_46=1372,21
PRIMER_LEFT_46_TM=60.924
PRIMER_RIGHT_46_TM=58.411
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=5.9000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=519
PRIMER_PAIR_47_PENALTY=3.544623
PRIMER_LEFT_47_PENALTY=0.955975
PRIMER_RIGHT_47_PENALTY=2.588648
PRIMER_LEFT_47_SEQUENCE=GCAATTCTCACACGCCTCTC
PRIMER_RIGHT_47_SEQUENCE=TTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_47=467,20
PRIMER_RIGHT_47=1372,21
PRIMER_LEFT_47_TM=60.956
PRIMER_RIGHT_47_TM=58.411
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=906
PRIMER_PAIR_48_PENALTY=3.559648
PRIMER_LEFT_48_PENALTY=0.330865
PRIMER_RIGHT_48_PENALTY=3.228784
PRIMER_LEFT_48_SEQUENCE=TTCGATCTTTCGCTTGTTCC
PRIMER_RIGHT_48_SEQUENCE=ATTCAGTCGACAGTTTCGTTGT
PRIMER_LEFT_48=515,20
PRIMER_RIGHT_48=1373,22
PRIMER_LEFT_48_TM=60.331
PRIMER_RIGHT_48_TM=58.771
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=859
PRIMER_PAIR_49_PENALTY=3.581669
PRIMER_LEFT_49_PENALTY=0.159750
PRIMER_RIGHT_49_PENALTY=3.421919
PRIMER_LEFT_49_SEQUENCE=CAAGATTCACTGACGCCAAA
PRIMER_RIGHT_49_SEQUENCE=GGTATTCAGTCGACAGTTTCGTT
PRIMER_LEFT_49=880,20
PRIMER_RIGHT_49=1376,23
PRIMER_LEFT_49_TM=59.840
PRIMER_RIGHT_49_TM=59.578
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=43.478
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.8000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=497
=
SEQUENCE_ID=MAGIC.433_1_16006196_16006213_bur-0_ws-0_17295_15374_TRUE
SEQUENCE_TARGET=950,117
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTNNNTGACTTAGTTCTTTGNTTTATGTTCCTCGTTTGAAAACTTNTGGCANAATTAATTANATATCATTNTTCTCGTTGTTTCTNGTTTGTTATGTTAATATATGATTNGTTNANTNATGCTTAGTAGTGAANTGTATGNTGCTNTTTTTATTTAATGNTTTGTAGCTAATCATATGTTNATGTTATTTTAATAANCGATTATTACTTTTNTTTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTTACAAGTTTGTAAGNCCATTTAGNGNACTTTGNAGGNAGTANGCANTTTTTTNGNGGTTTTCTANTGTTCGANAAAAATNAATANNATNCTACNCCTGCTNANGTCAGCTGATGCGCTAACNATGGTTAACAAANNTTTAATCANAGCNNTTCNTTTNTTNTGGAGAGAGAATCAATNTGGCGGCTATTATTAAGANTTAGAGANNANTTGTTGTTNTGTNGATAGCGNCGACAANCTCGTTNTTTTTTNGCNCCCNANAANGAAACATGAGCGAGGAGGNNNNNNNNNNNNANNTATTGCGGTTATTAAATNTGATGATTGTTGTNAATTCGTGTTGATGGTTGTAAAATNTTATTGTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_9016844_9017059_hi-0_ler-0_5472_152283_TRUE
SEQUENCE_TARGET=950,315
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATANTGAATATAGNTACNAAATAATATAATGATANATTATAATATGACAAATCACAATTTATATNTTTATGTTGTTCGATTTGTGACTACNTATTTGTGTTTGAAAAGTAGTATGATATGTTTTATTNACTACAAGAAAGACAACATTNAATTCNTTTTTGTTTGAATAACGTAACTNAATCGACCACTAAACTATCATACACGTNCTTAAAAAATGATGTTAAGTTTAATGTGATCCCCAAGTGTTGNAATTGGGTTGAGTAATAAATAGAAGTGGACAAATGGTTANTNANTTATTTTCCTAGCTAAATTCAAGTATTTTTCAAAATTATTTTGTTTCAAANTAAAACTTNAAAGTCATAACAAATTCTANNGAGTTTTAAAAATGACCAACAAATTGAAAGTGTGGTTGTTGCAAATGNAACTTNGTGGAGATACGGTCAGTGACAGCCNNACACGACTCCATCGGTTCGATCAAATACCTCACGTAACCCGCCGATTCACTCGATCGTCTTTATTATTCTTTTCNATTTTCAATCATATTGAGCCTGATTGATAATTACAAGTCTCATTACAAACGTATTAGCAACGGTATACAGTCAATATCCGTCACTAAAAATGCTAATTGCAACGAAAAGTATTCTTCTTTCNCTATGTAACTATAATGTGATATAGAATTAGCCCAAAANAAATTGTNTATGATTAATTAGATCTAAAATAAACNATGGTCGTCATGTGACGTTGATCAANCGATAAAGAGTAGTCTGAAATTTACTAATNAAAGTCTTAATTTGTACTTTTTACGAATTATAGTTTAAATGGACACAAAAGTAAATAGATTAANAAAACTAGAAANACGTGGAAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_5_13048869_13048914_zu-0_wil-2_55380_1576179_TRUE
SEQUENCE_TARGET=950,145
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_17542746_17542937_ler-0_kn-0_142468_140787_TRUE
SEQUENCE_TARGET=950,291
SEQUENCE_TEMPLATE=GATTTCACTGATTGGGGATTTTCAAATCCGTTTTCATGGATCTGAAAGTGTTGTTNAAGATTCTCAGATCAAGTTTGATTTGGTTTCTCGGGGAAGNGAGAATCGGAGTAGANGGTATCGCCGGAGTTTGTAGATTTTTTTCCGGCNGGCGAAGGATTAGAAAACATNCTTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNTGTTCATAGNTCTTGTTGTNGATGGTGNCGGTCAAATTGATGATGTGGAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANGAATGTGTTGNGATNGTNAANCCGTTGATAAAGANAGTGATANTTTTATTAANTTTGAAGAGNTTAAGATNNTGATNANNTCTAANAAAGANTCTTTCTGATNTTACTNAAAANTTTAATAGTTTAANTNNNTTTTGGTATTTNNTTTGCTTTTTANNTTATNNACTCGNTTTTGATGATNACNTTTTTATCAAATTGTCAATGAANTANTTTGTTTNANAAAAAGCACATCAAAGCAANTCCATACAAAATAANGTAAATTTGATATCATCATTTCAGCCTCNATGCATTTCCAAATTCGAACCAAAGATACGTAAGTTATGTTNATTGACACAATACCANAAAANAATAATTTANACTTCACTATCTTTTGCTAAAACAATATAATCATTATTTTCAGCCTCNTTTGTACACGGTACGTGTTTAAANTTTAAATCTTCNTTTTGTAATACTAACGNNNNNNNNNNNNNNNNNNNNNAGTTTAAAGATGGATGTTCAAAATTTGTTCAAGCANTTTTTTGTAATTCTCCCGTCTTGCAAAAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_9894582_9894925_kn-0_tsu-0_268977_581787_TRUE
SEQUENCE_TARGET=950,443
SEQUENCE_TEMPLATE=AGTTAAAGCCTTATNGTTTGTGTTCTGTTGTATTCAGACTTGGTGANGCTTATTGAGNAAACNGTTAAGTCAGAGAACTTTCGTTTACCAAATAGACCAATATATTTAGTTGGAGAATCTATTGGAGCATGTCTTGCTTTAGATGTTGCAGCCAGAAATCCCAACATCGATCTTTCTCTGATCTTGGTTAATCCAGGTACANAAGTTCGCCTTATTTTGTCGGTNAATGTCACGAATGTTTCTCATCATAGACATTGNTTTGTTGCAGCCACACATGTCAACAACTTCATGGTGCAACCTNTATCAGGAATGCTAAATGTTTTACCNGATGGTNTTCCAACGCTATTGGAAGATATCTTTGNTTTTGGTTTTAAGCAAGGTACACTTTTGTTCTCTTCTCATATTNTATCTTGAATCATAAATTNNNNNNNNNNNNNNNNTCCTTGTNGATCAAGAACTTATTGAAACGTGACAAAAATGTATCTCTTTGTTACGGTAGGCGATCCATTAACTGGGATGTTAGACGCTTTGTCGAATGAATTTTCTGTCCAGCGAATNGGCGGAGTAGGTGGAGGGATGCTAAGAGATGTCCTTGCTGTTTCAGCTAATCTTCCTGTAAGTATTCCTTCATAGAAACTTATGATATATGCGTTTTTCAGTTTATAGCCTACANATTTTCAAGTGTTCATCTAGTATTGTGATCACAATATANTTTCAGACTCTTAGTAGGATGTTCCCTAAGGACACACTGCTTTGGANGCTGGAAATGCTTAAGTATGCTATTGCTTCTGTGAACTCTCACATANACTCAGTCAGAGCGGAAACACTCATACTTCTGAGGTCCANAAATTCTGAATATATANGANTTNTAAAGAGGTNTTAAGTNTAAGTGCTTATCTGGTTATGACTATTGCAGTGGACNCGATCATTGGCTCCTGAAGGAGGAAGACATTGACAGATACTCGCGCACGTTGCCAAAATGTATTGTCCGTAAGCTCGANGACAATGGACAGTTTCCCCTTTTGGTTAGTGATATCTCTTCATCCAATACTGATACTAGTTTNCTCATGTCAATTGAGAATATCGGATCATTCATAACTTACACTTCAACTATCTCTTTTACATCTCATNTTAGGAGGANGGTGTAGATCTTGCTACTATCATCAAGTGTACTTGTTTTTATCGCCGTGGGAAGTCTCACGATCACATTACGGATTACATTATGCCTACCACATTTGAGTTAAAACAACAAGTAGACGATCACCGGTANNNNCTGAGTTATATNAAACAAAATCAGAATATCAAGTAAAAAGTTATAGGGGATACTGAATGATATATTCCGCNGTGCAGATTGCTAATGGATGGTACTTCTCCTGTAATGCTGTCAACTCTAGAAGACGGNACAGTTGTAAGGAGCCTCGAAGGATTACCTTCAGANGGACCTGTTTTGTACGTTGGCTATCACATGATATTGGGATTTGAGTTAGCTCCAATGGTAATTCAACTCATGACAGAGAGGAACATTCACCTGCGGGGTTTGGCACATCCCATGCTATTTAAGAATCTCCAAGACTCATTAGTCGACACGAAGATGTTTGACAAATATAAGATAATGGGTGGAGTTCCAGTCTCCCATTTCAATATCTACAAACTACTGCGTGAAAAGGCTCATGTGCTTCTGTATCCTGGAGGTGTCCGTGAAGCTTTGCATAGAAANGTGATNTATAGTTTGTAAACTTTTTCCGGGTTTCTGCATTCTTGAAACTTGTTATTTTAGAGGCTTTAATGAACAAACAAAATTTGCAGGGTGAAGAATACAAGCTGTTTTGGCCAGAACGGTCCGAGTTTGTGAGAGTTGCATCTAAATTTGGAGCCAAAATTGTTCCTTTTGGTGTTGTTGGAGAAGACGATATCTGCGAAGTAAGCTTTCTCATNTGCTATACATTATAANTATCCGAACCTCGGTTTGGCGTAACTTTGGTTTGGACATTGAATGATTCTCAGATTGTCCTGGATTCTAATGATCAAAGGAACATCCCTATCCTCAAGGATTTAATGGAAAAGGCAACAAAGGACGCTGGCAACATAAGGTGTTGCATCACATACTCTATNTATAATTTGTTCTCATAATACTTCTTTCAAGTAATATTCATATGAACATTGATTTTGAACGTCAGGGAAGGCGATGAGAGCGAATTGGGAAACCAAGAGTGCTATTTCCCAGGACTTGTACCTAAGATTCCAGGGCGGTTCTACTATTACTTTGGCAAACCAATAGAAACAGCAGGTATGTACTTAGTCTTAATGTATGCTCTCTACTTCAAATCACTGACCTCTATACTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.730439
PRIMER_LEFT_0_PENALTY=1.344683
PRIMER_RIGHT_0_PENALTY=0.385756
PRIMER_LEFT_0_SEQUENCE=CTCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_0_SEQUENCE=ACGGACACCTCCAGGATACA
PRIMER_LEFT_0=807,21
PRIMER_RIGHT_0=1698,20
PRIMER_LEFT_0_TM=59.655
PRIMER_RIGHT_0_TM=60.386
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=5.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=892
PRIMER_PAIR_1_PENALTY=1.746109
PRIMER_LEFT_1_PENALTY=1.344683
PRIMER_RIGHT_1_PENALTY=0.401426
PRIMER_LEFT_1_SEQUENCE=CTCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_1_SEQUENCE=TATGCAAAGCTTCACGGACA
PRIMER_LEFT_1=807,21
PRIMER_RIGHT_1=1711,20
PRIMER_LEFT_1_TM=59.655
PRIMER_RIGHT_1_TM=60.401
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=905
PRIMER_PAIR_2_PENALTY=1.783565
PRIMER_LEFT_2_PENALTY=0.477044
PRIMER_RIGHT_2_PENALTY=1.306521
PRIMER_LEFT_2_SEQUENCE=GGAGTAGGTGGAGGGATGCT
PRIMER_RIGHT_2_SEQUENCE=TGGAGCTAACTCAAATCCCAA
PRIMER_LEFT_2=561,20
PRIMER_RIGHT_2=1491,21
PRIMER_LEFT_2_TM=60.477
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=931
PRIMER_PAIR_3_PENALTY=1.791281
PRIMER_LEFT_3_PENALTY=1.405525
PRIMER_RIGHT_3_PENALTY=0.385756
PRIMER_LEFT_3_SEQUENCE=AGTCAGAGCGGAAACACTCA
PRIMER_RIGHT_3_SEQUENCE=ACGGACACCTCCAGGATACA
PRIMER_LEFT_3=810,20
PRIMER_RIGHT_3=1698,20
PRIMER_LEFT_3_TM=58.594
PRIMER_RIGHT_3_TM=60.386
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=889
PRIMER_PAIR_4_PENALTY=1.791281
PRIMER_LEFT_4_PENALTY=1.405525
PRIMER_RIGHT_4_PENALTY=0.385756
PRIMER_LEFT_4_SEQUENCE=TCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_4_SEQUENCE=ACGGACACCTCCAGGATACA
PRIMER_LEFT_4=808,20
PRIMER_RIGHT_4=1698,20
PRIMER_LEFT_4_TM=58.594
PRIMER_RIGHT_4_TM=60.386
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=891
PRIMER_PAIR_5_PENALTY=1.791281
PRIMER_LEFT_5_PENALTY=1.405525
PRIMER_RIGHT_5_PENALTY=0.385756
PRIMER_LEFT_5_SEQUENCE=CTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_5_SEQUENCE=ACGGACACCTCCAGGATACA
PRIMER_LEFT_5=807,20
PRIMER_RIGHT_5=1698,20
PRIMER_LEFT_5_TM=58.594
PRIMER_RIGHT_5_TM=60.386
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=5.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=892
PRIMER_PAIR_6_PENALTY=1.791281
PRIMER_LEFT_6_PENALTY=1.405525
PRIMER_RIGHT_6_PENALTY=0.385756
PRIMER_LEFT_6_SEQUENCE=ACTCAGTCAGAGCGGAAACA
PRIMER_RIGHT_6_SEQUENCE=ACGGACACCTCCAGGATACA
PRIMER_LEFT_6=806,20
PRIMER_RIGHT_6=1698,20
PRIMER_LEFT_6_TM=58.594
PRIMER_RIGHT_6_TM=60.386
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=5.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=893
PRIMER_PAIR_7_PENALTY=1.806950
PRIMER_LEFT_7_PENALTY=1.405525
PRIMER_RIGHT_7_PENALTY=0.401426
PRIMER_LEFT_7_SEQUENCE=AGTCAGAGCGGAAACACTCA
PRIMER_RIGHT_7_SEQUENCE=TATGCAAAGCTTCACGGACA
PRIMER_LEFT_7=810,20
PRIMER_RIGHT_7=1711,20
PRIMER_LEFT_7_TM=58.594
PRIMER_RIGHT_7_TM=60.401
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=902
PRIMER_PAIR_8_PENALTY=1.806950
PRIMER_LEFT_8_PENALTY=1.405525
PRIMER_RIGHT_8_PENALTY=0.401426
PRIMER_LEFT_8_SEQUENCE=TCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_8_SEQUENCE=TATGCAAAGCTTCACGGACA
PRIMER_LEFT_8=808,20
PRIMER_RIGHT_8=1711,20
PRIMER_LEFT_8_TM=58.594
PRIMER_RIGHT_8_TM=60.401
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=904
PRIMER_PAIR_9_PENALTY=1.806950
PRIMER_LEFT_9_PENALTY=1.405525
PRIMER_RIGHT_9_PENALTY=0.401426
PRIMER_LEFT_9_SEQUENCE=CTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_9_SEQUENCE=TATGCAAAGCTTCACGGACA
PRIMER_LEFT_9=807,20
PRIMER_RIGHT_9=1711,20
PRIMER_LEFT_9_TM=58.594
PRIMER_RIGHT_9_TM=60.401
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=905
PRIMER_PAIR_10_PENALTY=1.806950
PRIMER_LEFT_10_PENALTY=1.405525
PRIMER_RIGHT_10_PENALTY=0.401426
PRIMER_LEFT_10_SEQUENCE=ACTCAGTCAGAGCGGAAACA
PRIMER_RIGHT_10_SEQUENCE=TATGCAAAGCTTCACGGACA
PRIMER_LEFT_10=806,20
PRIMER_RIGHT_10=1711,20
PRIMER_LEFT_10_TM=58.594
PRIMER_RIGHT_10_TM=60.401
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=906
PRIMER_PAIR_11_PENALTY=1.868338
PRIMER_LEFT_11_PENALTY=0.761472
PRIMER_RIGHT_11_PENALTY=1.106866
PRIMER_LEFT_11_SEQUENCE=GGTGGAGGGATGCTAAGAGA
PRIMER_RIGHT_11_SEQUENCE=CCTTCGAGGCTCCTTACAACT
PRIMER_LEFT_11=567,20
PRIMER_RIGHT_11=1424,21
PRIMER_LEFT_11_TM=59.239
PRIMER_RIGHT_11_TM=59.893
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=858
PRIMER_PAIR_12_PENALTY=1.871934
PRIMER_LEFT_12_PENALTY=1.486178
PRIMER_RIGHT_12_PENALTY=0.385756
PRIMER_LEFT_12_SEQUENCE=ACTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_12_SEQUENCE=ACGGACACCTCCAGGATACA
PRIMER_LEFT_12=806,21
PRIMER_RIGHT_12=1698,20
PRIMER_LEFT_12_TM=59.514
PRIMER_RIGHT_12_TM=60.386
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=5.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=893
PRIMER_PAIR_13_PENALTY=1.887604
PRIMER_LEFT_13_PENALTY=1.486178
PRIMER_RIGHT_13_PENALTY=0.401426
PRIMER_LEFT_13_SEQUENCE=ACTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_13_SEQUENCE=TATGCAAAGCTTCACGGACA
PRIMER_LEFT_13=806,21
PRIMER_RIGHT_13=1711,20
PRIMER_LEFT_13_TM=59.514
PRIMER_RIGHT_13_TM=60.401
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=906
PRIMER_PAIR_14_PENALTY=2.067993
PRIMER_LEFT_14_PENALTY=0.761472
PRIMER_RIGHT_14_PENALTY=1.306521
PRIMER_LEFT_14_SEQUENCE=GGTGGAGGGATGCTAAGAGA
PRIMER_RIGHT_14_SEQUENCE=TGGAGCTAACTCAAATCCCAA
PRIMER_LEFT_14=567,20
PRIMER_RIGHT_14=1491,21
PRIMER_LEFT_14_TM=59.239
PRIMER_RIGHT_14_TM=59.693
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=10.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=925
PRIMER_PAIR_15_PENALTY=2.124817
PRIMER_LEFT_15_PENALTY=1.344683
PRIMER_RIGHT_15_PENALTY=0.780133
PRIMER_LEFT_15_SEQUENCE=CTCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_15_SEQUENCE=TGAGCCTTTTCACGCAGTAG
PRIMER_LEFT_15=807,21
PRIMER_RIGHT_15=1670,20
PRIMER_LEFT_15_TM=59.655
PRIMER_RIGHT_15_TM=59.220
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=5.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=864
PRIMER_PAIR_16_PENALTY=2.135920
PRIMER_LEFT_16_PENALTY=1.069554
PRIMER_RIGHT_16_PENALTY=1.066365
PRIMER_LEFT_16_SEQUENCE=GTTACGGTAGGCGATCCATT
PRIMER_RIGHT_16_SEQUENCE=CCTTCGAGGCTCCTTACAAC
PRIMER_LEFT_16=489,20
PRIMER_RIGHT_16=1424,20
PRIMER_LEFT_16_TM=58.930
PRIMER_RIGHT_16_TM=58.934
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=936
PRIMER_PAIR_17_PENALTY=2.175930
PRIMER_LEFT_17_PENALTY=1.109565
PRIMER_RIGHT_17_PENALTY=1.066365
PRIMER_LEFT_17_SEQUENCE=TCTCTTTGTTACGGTAGGCGA
PRIMER_RIGHT_17_SEQUENCE=CCTTCGAGGCTCCTTACAAC
PRIMER_LEFT_17=482,21
PRIMER_RIGHT_17=1424,20
PRIMER_LEFT_17_TM=59.890
PRIMER_RIGHT_17_TM=58.934
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=11.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=943
PRIMER_PAIR_18_PENALTY=2.176420
PRIMER_LEFT_18_PENALTY=1.069554
PRIMER_RIGHT_18_PENALTY=1.106866
PRIMER_LEFT_18_SEQUENCE=GTTACGGTAGGCGATCCATT
PRIMER_RIGHT_18_SEQUENCE=CCTTCGAGGCTCCTTACAACT
PRIMER_LEFT_18=489,20
PRIMER_RIGHT_18=1424,21
PRIMER_LEFT_18_TM=58.930
PRIMER_RIGHT_18_TM=59.893
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=936
PRIMER_PAIR_19_PENALTY=2.177677
PRIMER_LEFT_19_PENALTY=1.344683
PRIMER_RIGHT_19_PENALTY=0.832993
PRIMER_LEFT_19_SEQUENCE=CTCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_19_SEQUENCE=CAAAGCTTCACGGACACCTC
PRIMER_LEFT_19=807,21
PRIMER_RIGHT_19=1707,20
PRIMER_LEFT_19_TM=59.655
PRIMER_RIGHT_19_TM=60.833
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.1000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=901
PRIMER_PAIR_20_PENALTY=2.179341
PRIMER_LEFT_20_PENALTY=0.477044
PRIMER_RIGHT_20_PENALTY=1.702298
PRIMER_LEFT_20_SEQUENCE=GGAGTAGGTGGAGGGATGCT
PRIMER_RIGHT_20_SEQUENCE=TGGAGCTAACTCAAATCCCA
PRIMER_LEFT_20=561,20
PRIMER_RIGHT_20=1491,20
PRIMER_LEFT_20_TM=60.477
PRIMER_RIGHT_20_TM=58.298
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=931
PRIMER_PAIR_21_PENALTY=2.183113
PRIMER_LEFT_21_PENALTY=1.069554
PRIMER_RIGHT_21_PENALTY=1.113558
PRIMER_LEFT_21_SEQUENCE=GTTACGGTAGGCGATCCATT
PRIMER_RIGHT_21_SEQUENCE=ATCCTTCGAGGCTCCTTACA
PRIMER_LEFT_21=489,20
PRIMER_RIGHT_21=1426,20
PRIMER_LEFT_21_TM=58.930
PRIMER_RIGHT_21_TM=58.886
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=6.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=938
PRIMER_PAIR_22_PENALTY=2.185658
PRIMER_LEFT_22_PENALTY=1.405525
PRIMER_RIGHT_22_PENALTY=0.780133
PRIMER_LEFT_22_SEQUENCE=AGTCAGAGCGGAAACACTCA
PRIMER_RIGHT_22_SEQUENCE=TGAGCCTTTTCACGCAGTAG
PRIMER_LEFT_22=810,20
PRIMER_RIGHT_22=1670,20
PRIMER_LEFT_22_TM=58.594
PRIMER_RIGHT_22_TM=59.220
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=5.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=861
PRIMER_PAIR_23_PENALTY=2.185658
PRIMER_LEFT_23_PENALTY=1.405525
PRIMER_RIGHT_23_PENALTY=0.780133
PRIMER_LEFT_23_SEQUENCE=TCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_23_SEQUENCE=TGAGCCTTTTCACGCAGTAG
PRIMER_LEFT_23=808,20
PRIMER_RIGHT_23=1670,20
PRIMER_LEFT_23_TM=58.594
PRIMER_RIGHT_23_TM=59.220
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.1000
PRIMER_RIGHT_23_END_STABILITY=5.4000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=863
PRIMER_PAIR_24_PENALTY=2.185658
PRIMER_LEFT_24_PENALTY=1.405525
PRIMER_RIGHT_24_PENALTY=0.780133
PRIMER_LEFT_24_SEQUENCE=CTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_24_SEQUENCE=TGAGCCTTTTCACGCAGTAG
PRIMER_LEFT_24=807,20
PRIMER_RIGHT_24=1670,20
PRIMER_LEFT_24_TM=58.594
PRIMER_RIGHT_24_TM=59.220
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=5.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=864
PRIMER_PAIR_25_PENALTY=2.185658
PRIMER_LEFT_25_PENALTY=1.405525
PRIMER_RIGHT_25_PENALTY=0.780133
PRIMER_LEFT_25_SEQUENCE=ACTCAGTCAGAGCGGAAACA
PRIMER_RIGHT_25_SEQUENCE=TGAGCCTTTTCACGCAGTAG
PRIMER_LEFT_25=806,20
PRIMER_RIGHT_25=1670,20
PRIMER_LEFT_25_TM=58.594
PRIMER_RIGHT_25_TM=59.220
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=5.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=865
PRIMER_PAIR_26_PENALTY=2.191427
PRIMER_LEFT_26_PENALTY=1.125062
PRIMER_RIGHT_26_PENALTY=1.066365
PRIMER_LEFT_26_SEQUENCE=TGGGATGTTAGACGCTTTGTC
PRIMER_RIGHT_26_SEQUENCE=CCTTCGAGGCTCCTTACAAC
PRIMER_LEFT_26=512,21
PRIMER_RIGHT_26=1424,20
PRIMER_LEFT_26_TM=60.125
PRIMER_RIGHT_26_TM=58.934
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=913
PRIMER_PAIR_27_PENALTY=2.216430
PRIMER_LEFT_27_PENALTY=1.109565
PRIMER_RIGHT_27_PENALTY=1.106866
PRIMER_LEFT_27_SEQUENCE=TCTCTTTGTTACGGTAGGCGA
PRIMER_RIGHT_27_SEQUENCE=CCTTCGAGGCTCCTTACAACT
PRIMER_LEFT_27=482,21
PRIMER_RIGHT_27=1424,21
PRIMER_LEFT_27_TM=59.890
PRIMER_RIGHT_27_TM=59.893
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=11.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=943
PRIMER_PAIR_28_PENALTY=2.223123
PRIMER_LEFT_28_PENALTY=1.109565
PRIMER_RIGHT_28_PENALTY=1.113558
PRIMER_LEFT_28_SEQUENCE=TCTCTTTGTTACGGTAGGCGA
PRIMER_RIGHT_28_SEQUENCE=ATCCTTCGAGGCTCCTTACA
PRIMER_LEFT_28=482,21
PRIMER_RIGHT_28=1426,20
PRIMER_LEFT_28_TM=59.890
PRIMER_RIGHT_28_TM=58.886
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=11.4000
PRIMER_RIGHT_28_END_STABILITY=6.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=945
PRIMER_PAIR_29_PENALTY=2.231928
PRIMER_LEFT_29_PENALTY=1.125062
PRIMER_RIGHT_29_PENALTY=1.106866
PRIMER_LEFT_29_SEQUENCE=TGGGATGTTAGACGCTTTGTC
PRIMER_RIGHT_29_SEQUENCE=CCTTCGAGGCTCCTTACAACT
PRIMER_LEFT_29=512,21
PRIMER_RIGHT_29=1424,21
PRIMER_LEFT_29_TM=60.125
PRIMER_RIGHT_29_TM=59.893
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=913
PRIMER_PAIR_30_PENALTY=2.238518
PRIMER_LEFT_30_PENALTY=1.405525
PRIMER_RIGHT_30_PENALTY=0.832993
PRIMER_LEFT_30_SEQUENCE=AGTCAGAGCGGAAACACTCA
PRIMER_RIGHT_30_SEQUENCE=CAAAGCTTCACGGACACCTC
PRIMER_LEFT_30=810,20
PRIMER_RIGHT_30=1707,20
PRIMER_LEFT_30_TM=58.594
PRIMER_RIGHT_30_TM=60.833
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=898
PRIMER_PAIR_31_PENALTY=2.238518
PRIMER_LEFT_31_PENALTY=1.405525
PRIMER_RIGHT_31_PENALTY=0.832993
PRIMER_LEFT_31_SEQUENCE=TCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_31_SEQUENCE=CAAAGCTTCACGGACACCTC
PRIMER_LEFT_31=808,20
PRIMER_RIGHT_31=1707,20
PRIMER_LEFT_31_TM=58.594
PRIMER_RIGHT_31_TM=60.833
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.1000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=900
PRIMER_PAIR_32_PENALTY=2.238518
PRIMER_LEFT_32_PENALTY=1.405525
PRIMER_RIGHT_32_PENALTY=0.832993
PRIMER_LEFT_32_SEQUENCE=CTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_32_SEQUENCE=CAAAGCTTCACGGACACCTC
PRIMER_LEFT_32=807,20
PRIMER_RIGHT_32=1707,20
PRIMER_LEFT_32_TM=58.594
PRIMER_RIGHT_32_TM=60.833
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=901
PRIMER_PAIR_33_PENALTY=2.238518
PRIMER_LEFT_33_PENALTY=1.405525
PRIMER_RIGHT_33_PENALTY=0.832993
PRIMER_LEFT_33_SEQUENCE=ACTCAGTCAGAGCGGAAACA
PRIMER_RIGHT_33_SEQUENCE=CAAAGCTTCACGGACACCTC
PRIMER_LEFT_33=806,20
PRIMER_RIGHT_33=1707,20
PRIMER_LEFT_33_TM=58.594
PRIMER_RIGHT_33_TM=60.833
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=7.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=902
PRIMER_PAIR_34_PENALTY=2.238621
PRIMER_LEFT_34_PENALTY=1.125062
PRIMER_RIGHT_34_PENALTY=1.113558
PRIMER_LEFT_34_SEQUENCE=TGGGATGTTAGACGCTTTGTC
PRIMER_RIGHT_34_SEQUENCE=ATCCTTCGAGGCTCCTTACA
PRIMER_LEFT_34=512,21
PRIMER_RIGHT_34=1426,20
PRIMER_LEFT_34_TM=60.125
PRIMER_RIGHT_34_TM=58.886
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=6.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=915
PRIMER_PAIR_35_PENALTY=2.266311
PRIMER_LEFT_35_PENALTY=1.486178
PRIMER_RIGHT_35_PENALTY=0.780133
PRIMER_LEFT_35_SEQUENCE=ACTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_35_SEQUENCE=TGAGCCTTTTCACGCAGTAG
PRIMER_LEFT_35=806,21
PRIMER_RIGHT_35=1670,20
PRIMER_LEFT_35_TM=59.514
PRIMER_RIGHT_35_TM=59.220
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=5.4000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=865
PRIMER_PAIR_36_PENALTY=2.282443
PRIMER_LEFT_36_PENALTY=1.344683
PRIMER_RIGHT_36_PENALTY=0.937760
PRIMER_LEFT_36_SEQUENCE=CTCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_36_SEQUENCE=TTCTATGCAAAGCTTCACGG
PRIMER_LEFT_36=807,21
PRIMER_RIGHT_36=1714,20
PRIMER_LEFT_36_TM=59.655
PRIMER_RIGHT_36_TM=59.062
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.1000
PRIMER_RIGHT_36_END_STABILITY=9.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=908
PRIMER_PAIR_37_PENALTY=2.283949
PRIMER_LEFT_37_PENALTY=1.069554
PRIMER_RIGHT_37_PENALTY=1.214394
PRIMER_LEFT_37_SEQUENCE=GTTACGGTAGGCGATCCATT
PRIMER_RIGHT_37_SEQUENCE=AATCCTTCGAGGCTCCTTACA
PRIMER_LEFT_37=489,20
PRIMER_RIGHT_37=1427,21
PRIMER_LEFT_37_TM=58.930
PRIMER_RIGHT_37_TM=60.214
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=6.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=939
PRIMER_PAIR_38_PENALTY=2.317723
PRIMER_LEFT_38_PENALTY=0.477044
PRIMER_RIGHT_38_PENALTY=1.840679
PRIMER_LEFT_38_SEQUENCE=GGAGTAGGTGGAGGGATGCT
PRIMER_RIGHT_38_SEQUENCE=AGCCAACGTACAAAACAGGTC
PRIMER_LEFT_38=561,20
PRIMER_RIGHT_38=1459,21
PRIMER_LEFT_38_TM=60.477
PRIMER_RIGHT_38_TM=59.159
PRIMER_LEFT_38_GC_PERCENT=60.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=899
PRIMER_PAIR_39_PENALTY=2.319171
PRIMER_LEFT_39_PENALTY=1.486178
PRIMER_RIGHT_39_PENALTY=0.832993
PRIMER_LEFT_39_SEQUENCE=ACTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_39_SEQUENCE=CAAAGCTTCACGGACACCTC
PRIMER_LEFT_39=806,21
PRIMER_RIGHT_39=1707,20
PRIMER_LEFT_39_TM=59.514
PRIMER_RIGHT_39_TM=60.833
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=902
PRIMER_PAIR_40_PENALTY=2.323959
PRIMER_LEFT_40_PENALTY=1.109565
PRIMER_RIGHT_40_PENALTY=1.214394
PRIMER_LEFT_40_SEQUENCE=TCTCTTTGTTACGGTAGGCGA
PRIMER_RIGHT_40_SEQUENCE=AATCCTTCGAGGCTCCTTACA
PRIMER_LEFT_40=482,21
PRIMER_RIGHT_40=1427,21
PRIMER_LEFT_40_TM=59.890
PRIMER_RIGHT_40_TM=60.214
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.4000
PRIMER_RIGHT_40_END_STABILITY=6.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=946
PRIMER_PAIR_41_PENALTY=2.325528
PRIMER_LEFT_41_PENALTY=1.218662
PRIMER_RIGHT_41_PENALTY=1.106866
PRIMER_LEFT_41_SEQUENCE=AGGTGGAGGGATGCTAAGAGA
PRIMER_RIGHT_41_SEQUENCE=CCTTCGAGGCTCCTTACAACT
PRIMER_LEFT_41=566,21
PRIMER_RIGHT_41=1424,21
PRIMER_LEFT_41_TM=60.219
PRIMER_RIGHT_41_TM=59.893
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=859
PRIMER_PAIR_42_PENALTY=2.339457
PRIMER_LEFT_42_PENALTY=1.125062
PRIMER_RIGHT_42_PENALTY=1.214394
PRIMER_LEFT_42_SEQUENCE=TGGGATGTTAGACGCTTTGTC
PRIMER_RIGHT_42_SEQUENCE=AATCCTTCGAGGCTCCTTACA
PRIMER_LEFT_42=512,21
PRIMER_RIGHT_42=1427,21
PRIMER_LEFT_42_TM=60.125
PRIMER_RIGHT_42_TM=60.214
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=916
PRIMER_PAIR_43_PENALTY=2.342848
PRIMER_LEFT_43_PENALTY=1.344683
PRIMER_RIGHT_43_PENALTY=0.998165
PRIMER_LEFT_43_SEQUENCE=CTCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_43_SEQUENCE=GAAGCACATGAGCCTTTTCA
PRIMER_LEFT_43=807,21
PRIMER_RIGHT_43=1678,20
PRIMER_LEFT_43_TM=59.655
PRIMER_RIGHT_43_TM=59.002
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.1000
PRIMER_RIGHT_43_END_STABILITY=7.3000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=872
PRIMER_PAIR_44_PENALTY=2.343284
PRIMER_LEFT_44_PENALTY=1.405525
PRIMER_RIGHT_44_PENALTY=0.937760
PRIMER_LEFT_44_SEQUENCE=AGTCAGAGCGGAAACACTCA
PRIMER_RIGHT_44_SEQUENCE=TTCTATGCAAAGCTTCACGG
PRIMER_LEFT_44=810,20
PRIMER_RIGHT_44=1714,20
PRIMER_LEFT_44_TM=58.594
PRIMER_RIGHT_44_TM=59.062
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=9.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=905
PRIMER_PAIR_45_PENALTY=2.343284
PRIMER_LEFT_45_PENALTY=1.405525
PRIMER_RIGHT_45_PENALTY=0.937760
PRIMER_LEFT_45_SEQUENCE=TCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_45_SEQUENCE=TTCTATGCAAAGCTTCACGG
PRIMER_LEFT_45=808,20
PRIMER_RIGHT_45=1714,20
PRIMER_LEFT_45_TM=58.594
PRIMER_RIGHT_45_TM=59.062
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.1000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=907
PRIMER_PAIR_46_PENALTY=2.343284
PRIMER_LEFT_46_PENALTY=1.405525
PRIMER_RIGHT_46_PENALTY=0.937760
PRIMER_LEFT_46_SEQUENCE=CTCAGTCAGAGCGGAAACAC
PRIMER_RIGHT_46_SEQUENCE=TTCTATGCAAAGCTTCACGG
PRIMER_LEFT_46=807,20
PRIMER_RIGHT_46=1714,20
PRIMER_LEFT_46_TM=58.594
PRIMER_RIGHT_46_TM=59.062
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=908
PRIMER_PAIR_47_PENALTY=2.343284
PRIMER_LEFT_47_PENALTY=1.405525
PRIMER_RIGHT_47_PENALTY=0.937760
PRIMER_LEFT_47_SEQUENCE=ACTCAGTCAGAGCGGAAACA
PRIMER_RIGHT_47_SEQUENCE=TTCTATGCAAAGCTTCACGG
PRIMER_LEFT_47=806,20
PRIMER_RIGHT_47=1714,20
PRIMER_LEFT_47_TM=58.594
PRIMER_RIGHT_47_TM=59.062
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=909
PRIMER_PAIR_48_PENALTY=2.345092
PRIMER_LEFT_48_PENALTY=0.477044
PRIMER_RIGHT_48_PENALTY=1.868048
PRIMER_LEFT_48_SEQUENCE=GGAGTAGGTGGAGGGATGCT
PRIMER_RIGHT_48_SEQUENCE=GCCAACGTACAAAACAGGTC
PRIMER_LEFT_48=561,20
PRIMER_RIGHT_48=1458,20
PRIMER_LEFT_48_TM=60.477
PRIMER_RIGHT_48_TM=58.132
PRIMER_LEFT_48_GC_PERCENT=60.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.1000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=898
PRIMER_PAIR_49_PENALTY=2.403689
PRIMER_LEFT_49_PENALTY=1.405525
PRIMER_RIGHT_49_PENALTY=0.998165
PRIMER_LEFT_49_SEQUENCE=TCAGTCAGAGCGGAAACACT
PRIMER_RIGHT_49_SEQUENCE=GAAGCACATGAGCCTTTTCA
PRIMER_LEFT_49=808,20
PRIMER_RIGHT_49=1678,20
PRIMER_LEFT_49_TM=58.594
PRIMER_RIGHT_49_TM=59.002
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.1000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=871
=
SEQUENCE_ID=MAGIC.175_3_13149578_13149620_ler-0_tsu-0_7664_2352_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_16676306_16677081_oy-0_can-0_359346_2666_TRUE
SEQUENCE_TARGET=950,875
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_4504570_4504783_ws-0_ler-0_1241_69707_TRUE
SEQUENCE_TARGET=950,313
SEQUENCE_TEMPLATE=GGATNTGTNATCNGNGTTCCNNAACTGAATGAACTTCTCAGCNTGAGGAGTTCTTCNAAGAAAGNNGNATACTTCTNGGCTTATNCGAANGCCNACANGAANCTCATCTNNNATCTTNCNAACAANGACGANAACTNGCANCANCCNTGGTTNNTAANNNNNNNNNNNNNNNNNNNNNTNGNAAATCTTTCCGACNTGCTCCCCNNGAAGNCNNGTAGNNCTTAGGTCATGCNTATGATTTTTATANANATNTTGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGAAACTCTTTGGAATTCCTTCACCNTNGACNGNTTCATANAGGCNAACCACAAGCTCANGANGAGTCNCCCTGNACAACTTCTTCAACTTCNGTTNCCTCCTCCTTTACCTCANNGANCNTCNNCTCGAGNCATCGCCNCCNGANGNAAGANGNTGTCCAAATCNATCGTGGAGGCTTGTGANGNGAACAANTNNTTCNTTACGTNGGNTNTNGANAAGAAGGAGTACAGNAGAACCCNGNTANTNNANGNTGGNAGTNCCNAAGGNGNNAGATCNGNNNNTGCCTTCAGACCCANNCCTCANNNTGAAGGTCATGGTNGNNNNTCACCTCNNNGAGACNNATCACCTCNTCGTGANTCACCTNNGCCTNCNTNTNGNAATAGGTTGAGCAGNGAANCNATCNCGNANTTNNTTNNNAAGAANAGNNAAANNCCANCNCNNNGCAGTTNNTCNNCTAAGAGNGATAAGACNANAGCCNGAACNGATCGTTCTCCTCNATCNTCTCCCCCACCTNGCTNGATGGNTNCCNNTCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTNCAAATCCTCGACANGAAGANGNNGAGNCCCGAGATNCTANCCCTAAACCCAAGGNCNNTNTGGTTNCTCAAACNAAATCACTCACTNGTNAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACAGCGATGNAAACAGAGGGAANNNNNNNNNNATGCTTCAAGACTAGGAAAGACACGANCTNNCCAACCTTTGANANGTGGCGTCCTGCGATTCNNGAGNGATTCNTACTTCNNGCNCATCACTCNTCTCGGGNNNNNNNNNNNNNNNNNNNNNNNNTNANCTCNACTCTTTNGCTTATACNCNAACTCACCTTTGTAANCNTTGNTTCAGGCAAACANCNNNCTCAATGACATGATTGAGTATTACGAAAGNNTGNTGCTTGNCANGGAANGANGTTATNGCCTNGAAAGANAAGTTTTCTTCTNTTGAGTCCGATCTCNNCTCTTCCNCTGAAGTCANGCNNAAACTNGAAGANCAATTGGACAANCTTTNTTCCGAACTCANNNNNNNNNNNNNGAGTTGNAGGNTCAGTATCANNGGTATGANAANATCNAGGAGGAACTCTCGAATGCTCNNGNCAGACTNTNAGNNNNNNNNNNNNNNNNTTACGACTTGTCCAACCAGCTATCCNAANTGCANGCCANNTACAAAGCAATNGCCAAACNTCGAGATGNTNAGTTANNNNNNNNNNNNNNAAAAGCCAGGAAAGANGTAAANGGTCGCGGGATGGAANTGATTCAANGGGCNATCGTNTTNATCNAAACCGAGAAAACTAGGACGNANNTGGAATCAGANATCAAGGANTATGAAATCAACCTCCTCCTTCTGNGNCAAACCCATGACGAAGATTTTTCNGAAGNNCNNNNNAGGTCCGAATTGNAAGCNGTTNTAACAGAGAANCNAANTCNTCTGGCCGCTCNNCCNTCTTNNTCATTCAACCNTCAANNNNNNNNAGAGTTCTTGACCGAGTCGCCTCCTTTGAGCGAGTCNGGTTTAGATTNNNNNNNNNNNTTTTAAAACCTCAANTTNCGANNNNNNNNNNNNNNANNTTANACTTGCGTTTTCNTGATTCGAGATGATTNTCTCANGNNCCAGTGATCCCNTGGAACCNGAGGTATCNATCATGCCGNTNGANACTCCNANNNNGGTAGCCATCCCNNCAGAAATTCCTCCACCCACGAACACNGAAACAGTGGTNATCGAGGATNATGATGGTTCGNACNC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_13159524_13159533_tsu-0_wu-0_5503_8291_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_10337713_10337773_zu-0_mt-0_1320552_166068_TRUE
SEQUENCE_TARGET=950,160
SEQUENCE_TEMPLATE=ATAACACTTATACCGGATGATAATGTGACGCTTTGGTCTAATGGTGGAAGAAAATCGTTTGATTTGATTTTGATAGCGATTACAAGTCTCTTTTGNTTGGCTCATAAGACTTCGTCTAAAAAAGTGATAACGACTTCTAGAAGTATTTTTATACGTACATTTACGTGCCCATGNGAGAAATTTGCTTNTGNACAGTATCNTATTTNTTGCNGTCAATTATTTTATCTTGCGNTATAAAATGCANCAAAANCAAACAATTTCGAACGTTCGTTTTCGAGAAGCTAAGATTAAACTACATTAAAAAGGAAAAATTTGATTCAACGCAGCAGCCATGATTCATGATTCATGATTTCTATTGGCTTTGTCNGCAATTACGTATGCATTTTCCTNTATTGAGTAGATTTGGACTAAAATTCAACTTTACACANACACACACATATAAACCACATAAATAAATTTATGGTTAATTGATATAAAACATGAAAATGTTACTTTAACATGGCTGTTGAAGTAATTGACGTAAGTGATAGNGATGATTTATGCCCTATNTATTATGTAAATATAATATCGTACTAATATNTACGTGTCCAAAGGTTCTTGAATGGTCGACATTTTGTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATACAAAATTTNTTTGAGTTAAAGAATCATACATATTTCCGCCNATTAATCAACGAATTCCAAGTTTAATAACGTNAAGACCATCACTAATTTTCAATAGTAAACTTTTNAGTTAGGCGAACTGACAAACTGAATGTACCATAGCAAAAANAACAGATCGAATAAANNTTTTGAAAATNATCGAATGNATATTAAATNTTAGAACAAAAATAANTGGAANCGATTTGATANCGAANNAAAGTATTTTANGNANNTTAAAATATTTGAAATACAGTTATATTCAAAAATATTANTTANTTATAGACCAAANAATNAAAAATNTTTNNANNTATATAAAAATANTAAAATATTTGAAAATAATNNNAAAAATATCNGAAAGTACNCCAAATAANCAAAAATATCNNAAAATATTCAAAATAAAATAACTGAATAGATACAAAATTTTAAAANCGAAAGGANNAAAACCAAANNAAAAACNGAANTAAAAANNTAGTAAANTCTAAAATTAAAANCNAATGAACCAAACTNANAANCAAANGNCNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCAAAACTTGTGGATCAAGTAGCATGATTCGCTTCTAGAAAATCCACGTTATGCTTCTTCTCTAAATCTTTTGATACAAATACACAATTGAGTCACATTAATAACTATCTTACACTTTAATTACCAAAATTGCCATTTCCATGTGCCCCCAACCACTTCCTCCTATATATACGGCTATTTATACAACAAGANAAAAAAAAAAAANCTCCATTAAAGCAAAAAACTCCNAAANCTCTCTCTCTTTCTTCCTCACACGCTTTTTTCATTTCTCAACTGNAAAAAATCTGATCCAATGGAGAAAGACGATGCAACGGCGTCGTTTTCACCTAGCTTCAGTACTTATTCCGGCGATNGACTCGTCGAAATCGCCGAACGAGTCTGTTACAAAGAGAAATCAGACGCCGAATTCGAGTTTTCGACGGCGGCGANNNCTTNTTCCGGTGACGGTGGACTAGTTTTCCCTGTATTTAACAAGAATCTAATCTCCGGAGANGTTTCNCCGGAGAAAGTAATTCCGTTGAAGGATCTATTCCTCCGTGAACGTAACGATCAACAACCACCGCAACAAACATACTCATCGTCTTCCGACGAAGAAGAAGAAGACGACGAGTTTGATTCGATCCCGAGCGAGATCTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.685845
PRIMER_LEFT_0_PENALTY=1.503187
PRIMER_RIGHT_0_PENALTY=0.182658
PRIMER_LEFT_0_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_0_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_0=861,20
PRIMER_RIGHT_0=1589,20
PRIMER_LEFT_0_TM=58.497
PRIMER_RIGHT_0_TM=60.183
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=729
PRIMER_PAIR_1_PENALTY=1.728426
PRIMER_LEFT_1_PENALTY=1.683594
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_1_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_1=862,21
PRIMER_RIGHT_1=1828,20
PRIMER_LEFT_1_TM=60.684
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=967
PRIMER_PAIR_2_PENALTY=1.728426
PRIMER_LEFT_2_PENALTY=1.683594
PRIMER_RIGHT_2_PENALTY=0.044832
PRIMER_LEFT_2_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_2_SEQUENCE=TTCGGCGTCTGATTTCTCTT
PRIMER_LEFT_2=862,21
PRIMER_RIGHT_2=1829,20
PRIMER_LEFT_2_TM=60.684
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=968
PRIMER_PAIR_3_PENALTY=1.866252
PRIMER_LEFT_3_PENALTY=1.683594
PRIMER_RIGHT_3_PENALTY=0.182658
PRIMER_LEFT_3_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_3_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_3=862,21
PRIMER_RIGHT_3=1589,20
PRIMER_LEFT_3_TM=60.684
PRIMER_RIGHT_3_TM=60.183
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=728
PRIMER_PAIR_4_PENALTY=2.262981
PRIMER_LEFT_4_PENALTY=2.080323
PRIMER_RIGHT_4_PENALTY=0.182658
PRIMER_LEFT_4_SEQUENCE=TTAGGCGAACTGACAAACTGAA
PRIMER_RIGHT_4_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_4=859,22
PRIMER_RIGHT_4=1589,20
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.183
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=731
PRIMER_PAIR_5_PENALTY=2.478557
PRIMER_LEFT_5_PENALTY=2.433726
PRIMER_RIGHT_5_PENALTY=0.044832
PRIMER_LEFT_5_SEQUENCE=TTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_5_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_5=859,21
PRIMER_RIGHT_5=1828,20
PRIMER_LEFT_5_TM=58.566
PRIMER_RIGHT_5_TM=59.955
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=970
PRIMER_PAIR_6_PENALTY=2.478557
PRIMER_LEFT_6_PENALTY=2.433726
PRIMER_RIGHT_6_PENALTY=0.044832
PRIMER_LEFT_6_SEQUENCE=TTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_6_SEQUENCE=TTCGGCGTCTGATTTCTCTT
PRIMER_LEFT_6=859,21
PRIMER_RIGHT_6=1829,20
PRIMER_LEFT_6_TM=58.566
PRIMER_RIGHT_6_TM=59.955
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=971
PRIMER_PAIR_7_PENALTY=2.587214
PRIMER_LEFT_7_PENALTY=1.683594
PRIMER_RIGHT_7_PENALTY=0.903619
PRIMER_LEFT_7_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_7_SEQUENCE=CGGCGTCTGATTTCTCTTTG
PRIMER_LEFT_7=862,21
PRIMER_RIGHT_7=1827,20
PRIMER_LEFT_7_TM=60.684
PRIMER_RIGHT_7_TM=60.904
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=966
PRIMER_PAIR_8_PENALTY=2.614044
PRIMER_LEFT_8_PENALTY=1.503187
PRIMER_RIGHT_8_PENALTY=1.110857
PRIMER_LEFT_8_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_8_SEQUENCE=ATTCGGCGTCTGATTTCTCT
PRIMER_LEFT_8=861,20
PRIMER_RIGHT_8=1830,20
PRIMER_LEFT_8_TM=58.497
PRIMER_RIGHT_8_TM=58.889
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=970
PRIMER_PAIR_9_PENALTY=2.616384
PRIMER_LEFT_9_PENALTY=2.433726
PRIMER_RIGHT_9_PENALTY=0.182658
PRIMER_LEFT_9_SEQUENCE=TAGGCGAACTGACAAACTGAA
PRIMER_RIGHT_9_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_9=860,21
PRIMER_RIGHT_9=1589,20
PRIMER_LEFT_9_TM=58.566
PRIMER_RIGHT_9_TM=60.183
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=11.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=730
PRIMER_PAIR_10_PENALTY=2.616384
PRIMER_LEFT_10_PENALTY=2.433726
PRIMER_RIGHT_10_PENALTY=0.182658
PRIMER_LEFT_10_SEQUENCE=TTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_10_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_10=859,21
PRIMER_RIGHT_10=1589,20
PRIMER_LEFT_10_TM=58.566
PRIMER_RIGHT_10_TM=60.183
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=11.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=731
PRIMER_PAIR_11_PENALTY=2.620403
PRIMER_LEFT_11_PENALTY=2.575571
PRIMER_RIGHT_11_PENALTY=0.044832
PRIMER_LEFT_11_SEQUENCE=GTTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_11_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_11=858,22
PRIMER_RIGHT_11=1828,20
PRIMER_LEFT_11_TM=59.424
PRIMER_RIGHT_11_TM=59.955
PRIMER_LEFT_11_GC_PERCENT=45.455
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=971
PRIMER_PAIR_12_PENALTY=2.620403
PRIMER_LEFT_12_PENALTY=2.575571
PRIMER_RIGHT_12_PENALTY=0.044832
PRIMER_LEFT_12_SEQUENCE=GTTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_12_SEQUENCE=TTCGGCGTCTGATTTCTCTT
PRIMER_LEFT_12=858,22
PRIMER_RIGHT_12=1829,20
PRIMER_LEFT_12_TM=59.424
PRIMER_RIGHT_12_TM=59.955
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=972
PRIMER_PAIR_13_PENALTY=2.689496
PRIMER_LEFT_13_PENALTY=1.503187
PRIMER_RIGHT_13_PENALTY=1.186309
PRIMER_LEFT_13_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_13_SEQUENCE=TATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_13=861,20
PRIMER_RIGHT_13=1590,21
PRIMER_LEFT_13_TM=58.497
PRIMER_RIGHT_13_TM=60.186
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=11.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=730
PRIMER_PAIR_14_PENALTY=2.725051
PRIMER_LEFT_14_PENALTY=1.503187
PRIMER_RIGHT_14_PENALTY=1.221865
PRIMER_LEFT_14_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_14_SEQUENCE=AATTCGGCGTCTGATTTCTCT
PRIMER_LEFT_14=861,20
PRIMER_RIGHT_14=1831,21
PRIMER_LEFT_14_TM=58.497
PRIMER_RIGHT_14_TM=60.222
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=971
PRIMER_PAIR_15_PENALTY=2.758229
PRIMER_LEFT_15_PENALTY=2.575571
PRIMER_RIGHT_15_PENALTY=0.182658
PRIMER_LEFT_15_SEQUENCE=GTTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_15_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_15=858,22
PRIMER_RIGHT_15=1589,20
PRIMER_LEFT_15_TM=59.424
PRIMER_RIGHT_15_TM=60.183
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=11.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=732
PRIMER_PAIR_16_PENALTY=2.869903
PRIMER_LEFT_16_PENALTY=1.683594
PRIMER_RIGHT_16_PENALTY=1.186309
PRIMER_LEFT_16_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_16_SEQUENCE=TATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_16=862,21
PRIMER_RIGHT_16=1590,21
PRIMER_LEFT_16_TM=60.684
PRIMER_RIGHT_16_TM=60.186
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=11.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=729
PRIMER_PAIR_17_PENALTY=2.905459
PRIMER_LEFT_17_PENALTY=1.683594
PRIMER_RIGHT_17_PENALTY=1.221865
PRIMER_LEFT_17_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_17_SEQUENCE=AATTCGGCGTCTGATTTCTCT
PRIMER_LEFT_17=862,21
PRIMER_RIGHT_17=1831,21
PRIMER_LEFT_17_TM=60.684
PRIMER_RIGHT_17_TM=60.222
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=970
PRIMER_PAIR_18_PENALTY=2.990230
PRIMER_LEFT_18_PENALTY=2.945399
PRIMER_RIGHT_18_PENALTY=0.044832
PRIMER_LEFT_18_SEQUENCE=TAGGCGAACTGACAAACTGA
PRIMER_RIGHT_18_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_18=860,20
PRIMER_RIGHT_18=1828,20
PRIMER_LEFT_18_TM=57.055
PRIMER_RIGHT_18_TM=59.955
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=969
PRIMER_PAIR_19_PENALTY=2.990230
PRIMER_LEFT_19_PENALTY=2.945399
PRIMER_RIGHT_19_PENALTY=0.044832
PRIMER_LEFT_19_SEQUENCE=TAGGCGAACTGACAAACTGA
PRIMER_RIGHT_19_SEQUENCE=TTCGGCGTCTGATTTCTCTT
PRIMER_LEFT_19=860,20
PRIMER_RIGHT_19=1829,20
PRIMER_LEFT_19_TM=57.055
PRIMER_RIGHT_19_TM=59.955
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=970
PRIMER_PAIR_20_PENALTY=3.128057
PRIMER_LEFT_20_PENALTY=2.945399
PRIMER_RIGHT_20_PENALTY=0.182658
PRIMER_LEFT_20_SEQUENCE=TAGGCGAACTGACAAACTGA
PRIMER_RIGHT_20_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_20=860,20
PRIMER_RIGHT_20=1589,20
PRIMER_LEFT_20_TM=57.055
PRIMER_RIGHT_20_TM=60.183
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=11.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=730
PRIMER_PAIR_21_PENALTY=3.159029
PRIMER_LEFT_21_PENALTY=3.114197
PRIMER_RIGHT_21_PENALTY=0.044832
PRIMER_LEFT_21_SEQUENCE=GCGAACTGACAAACTGAATG
PRIMER_RIGHT_21_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_21=863,20
PRIMER_RIGHT_21=1828,20
PRIMER_LEFT_21_TM=56.886
PRIMER_RIGHT_21_TM=59.955
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=966
PRIMER_PAIR_22_PENALTY=3.159029
PRIMER_LEFT_22_PENALTY=3.114197
PRIMER_RIGHT_22_PENALTY=0.044832
PRIMER_LEFT_22_SEQUENCE=GCGAACTGACAAACTGAATG
PRIMER_RIGHT_22_SEQUENCE=TTCGGCGTCTGATTTCTCTT
PRIMER_LEFT_22=863,20
PRIMER_RIGHT_22=1829,20
PRIMER_LEFT_22_TM=56.886
PRIMER_RIGHT_22_TM=59.955
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.9000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=967
PRIMER_PAIR_23_PENALTY=3.191180
PRIMER_LEFT_23_PENALTY=2.080323
PRIMER_RIGHT_23_PENALTY=1.110857
PRIMER_LEFT_23_SEQUENCE=TTAGGCGAACTGACAAACTGAA
PRIMER_RIGHT_23_SEQUENCE=ATTCGGCGTCTGATTTCTCT
PRIMER_LEFT_23=859,22
PRIMER_RIGHT_23=1830,20
PRIMER_LEFT_23_TM=59.920
PRIMER_RIGHT_23_TM=58.889
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=972
PRIMER_PAIR_24_PENALTY=3.205068
PRIMER_LEFT_24_PENALTY=1.503187
PRIMER_RIGHT_24_PENALTY=1.701882
PRIMER_LEFT_24_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_24_SEQUENCE=TTGCATCGTCTTTCTCCATT
PRIMER_LEFT_24=861,20
PRIMER_RIGHT_24=1734,20
PRIMER_LEFT_24_TM=58.497
PRIMER_RIGHT_24_TM=58.298
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=874
PRIMER_PAIR_25_PENALTY=3.266632
PRIMER_LEFT_25_PENALTY=2.080323
PRIMER_RIGHT_25_PENALTY=1.186309
PRIMER_LEFT_25_SEQUENCE=TTAGGCGAACTGACAAACTGAA
PRIMER_RIGHT_25_SEQUENCE=TATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_25=859,22
PRIMER_RIGHT_25=1590,21
PRIMER_LEFT_25_TM=59.920
PRIMER_RIGHT_25_TM=60.186
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=52.381
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=11.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=732
PRIMER_PAIR_26_PENALTY=3.281101
PRIMER_LEFT_26_PENALTY=1.503187
PRIMER_RIGHT_26_PENALTY=1.777914
PRIMER_LEFT_26_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_26_SEQUENCE=GCGTGTGAGGAAGAAAGAGAG
PRIMER_LEFT_26=861,20
PRIMER_RIGHT_26=1680,21
PRIMER_LEFT_26_TM=58.497
PRIMER_RIGHT_26_TM=59.222
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=820
PRIMER_PAIR_27_PENALTY=3.281101
PRIMER_LEFT_27_PENALTY=1.503187
PRIMER_RIGHT_27_PENALTY=1.777914
PRIMER_LEFT_27_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_27_SEQUENCE=AGCGTGTGAGGAAGAAAGAGA
PRIMER_LEFT_27=861,20
PRIMER_RIGHT_27=1681,21
PRIMER_LEFT_27_TM=58.497
PRIMER_RIGHT_27_TM=59.222
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=821
PRIMER_PAIR_28_PENALTY=3.296855
PRIMER_LEFT_28_PENALTY=3.114197
PRIMER_RIGHT_28_PENALTY=0.182658
PRIMER_LEFT_28_SEQUENCE=GCGAACTGACAAACTGAATG
PRIMER_RIGHT_28_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_28=863,20
PRIMER_RIGHT_28=1589,20
PRIMER_LEFT_28_TM=56.886
PRIMER_RIGHT_28_TM=60.183
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=11.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=727
PRIMER_PAIR_29_PENALTY=3.302187
PRIMER_LEFT_29_PENALTY=2.080323
PRIMER_RIGHT_29_PENALTY=1.221865
PRIMER_LEFT_29_SEQUENCE=TTAGGCGAACTGACAAACTGAA
PRIMER_RIGHT_29_SEQUENCE=AATTCGGCGTCTGATTTCTCT
PRIMER_LEFT_29=859,22
PRIMER_RIGHT_29=1831,21
PRIMER_LEFT_29_TM=59.920
PRIMER_RIGHT_29_TM=60.222
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=973
PRIMER_PAIR_30_PENALTY=3.314971
PRIMER_LEFT_30_PENALTY=1.503187
PRIMER_RIGHT_30_PENALTY=1.811785
PRIMER_LEFT_30_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_30_SEQUENCE=GTTGCATCGTCTTTCTCCATT
PRIMER_LEFT_30=861,20
PRIMER_RIGHT_30=1735,21
PRIMER_LEFT_30_TM=58.497
PRIMER_RIGHT_30_TM=59.188
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=875
PRIMER_PAIR_31_PENALTY=3.345024
PRIMER_LEFT_31_PENALTY=1.503187
PRIMER_RIGHT_31_PENALTY=1.841837
PRIMER_LEFT_31_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_31_SEQUENCE=GCGTGTGAGGAAGAAAGAGA
PRIMER_LEFT_31=861,20
PRIMER_RIGHT_31=1680,20
PRIMER_LEFT_31_TM=58.497
PRIMER_RIGHT_31_TM=58.158
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=820
PRIMER_PAIR_32_PENALTY=3.391898
PRIMER_LEFT_32_PENALTY=1.503187
PRIMER_RIGHT_32_PENALTY=1.888711
PRIMER_LEFT_32_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_32_SEQUENCE=AAAGCGTGTGAGGAAGAAAGA
PRIMER_LEFT_32=861,20
PRIMER_RIGHT_32=1683,21
PRIMER_LEFT_32_TM=58.497
PRIMER_RIGHT_32_TM=59.111
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=823
PRIMER_PAIR_33_PENALTY=3.450888
PRIMER_LEFT_33_PENALTY=3.406057
PRIMER_RIGHT_33_PENALTY=0.044832
PRIMER_LEFT_33_SEQUENCE=TTAGGCGAACTGACAAACTG
PRIMER_RIGHT_33_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_33=859,20
PRIMER_RIGHT_33=1828,20
PRIMER_LEFT_33_TM=56.594
PRIMER_RIGHT_33_TM=59.955
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=970
PRIMER_PAIR_34_PENALTY=3.450888
PRIMER_LEFT_34_PENALTY=3.406057
PRIMER_RIGHT_34_PENALTY=0.044832
PRIMER_LEFT_34_SEQUENCE=TTAGGCGAACTGACAAACTG
PRIMER_RIGHT_34_SEQUENCE=TTCGGCGTCTGATTTCTCTT
PRIMER_LEFT_34=859,20
PRIMER_RIGHT_34=1829,20
PRIMER_LEFT_34_TM=56.594
PRIMER_RIGHT_34_TM=59.955
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=971
PRIMER_PAIR_35_PENALTY=3.461508
PRIMER_LEFT_35_PENALTY=1.683594
PRIMER_RIGHT_35_PENALTY=1.777914
PRIMER_LEFT_35_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_35_SEQUENCE=GCGTGTGAGGAAGAAAGAGAG
PRIMER_LEFT_35=862,21
PRIMER_RIGHT_35=1680,21
PRIMER_LEFT_35_TM=60.684
PRIMER_RIGHT_35_TM=59.222
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=819
PRIMER_PAIR_36_PENALTY=3.461508
PRIMER_LEFT_36_PENALTY=1.683594
PRIMER_RIGHT_36_PENALTY=1.777914
PRIMER_LEFT_36_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_36_SEQUENCE=AGCGTGTGAGGAAGAAAGAGA
PRIMER_LEFT_36=862,21
PRIMER_RIGHT_36=1681,21
PRIMER_LEFT_36_TM=60.684
PRIMER_RIGHT_36_TM=59.222
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=820
PRIMER_PAIR_37_PENALTY=3.488443
PRIMER_LEFT_37_PENALTY=3.443611
PRIMER_RIGHT_37_PENALTY=0.044832
PRIMER_LEFT_37_SEQUENCE=GTTAGGCGAACTGACAAACTG
PRIMER_RIGHT_37_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_37=858,21
PRIMER_RIGHT_37=1828,20
PRIMER_LEFT_37_TM=57.556
PRIMER_RIGHT_37_TM=59.955
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=971
PRIMER_PAIR_38_PENALTY=3.488443
PRIMER_LEFT_38_PENALTY=3.443611
PRIMER_RIGHT_38_PENALTY=0.044832
PRIMER_LEFT_38_SEQUENCE=GTTAGGCGAACTGACAAACTG
PRIMER_RIGHT_38_SEQUENCE=TTCGGCGTCTGATTTCTCTT
PRIMER_LEFT_38=858,21
PRIMER_RIGHT_38=1829,20
PRIMER_LEFT_38_TM=57.556
PRIMER_RIGHT_38_TM=59.955
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=972
PRIMER_PAIR_39_PENALTY=3.525431
PRIMER_LEFT_39_PENALTY=1.683594
PRIMER_RIGHT_39_PENALTY=1.841837
PRIMER_LEFT_39_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_39_SEQUENCE=GCGTGTGAGGAAGAAAGAGA
PRIMER_LEFT_39=862,21
PRIMER_RIGHT_39=1680,20
PRIMER_LEFT_39_TM=60.684
PRIMER_RIGHT_39_TM=58.158
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=819
PRIMER_PAIR_40_PENALTY=3.544583
PRIMER_LEFT_40_PENALTY=2.433726
PRIMER_RIGHT_40_PENALTY=1.110857
PRIMER_LEFT_40_SEQUENCE=TAGGCGAACTGACAAACTGAA
PRIMER_RIGHT_40_SEQUENCE=ATTCGGCGTCTGATTTCTCT
PRIMER_LEFT_40=860,21
PRIMER_RIGHT_40=1830,20
PRIMER_LEFT_40_TM=58.566
PRIMER_RIGHT_40_TM=58.889
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=971
PRIMER_PAIR_41_PENALTY=3.544583
PRIMER_LEFT_41_PENALTY=2.433726
PRIMER_RIGHT_41_PENALTY=1.110857
PRIMER_LEFT_41_SEQUENCE=TTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_41_SEQUENCE=ATTCGGCGTCTGATTTCTCT
PRIMER_LEFT_41=859,21
PRIMER_RIGHT_41=1830,20
PRIMER_LEFT_41_TM=58.566
PRIMER_RIGHT_41_TM=58.889
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=972
PRIMER_PAIR_42_PENALTY=3.557208
PRIMER_LEFT_42_PENALTY=1.503187
PRIMER_RIGHT_42_PENALTY=2.054021
PRIMER_LEFT_42_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_42_SEQUENCE=AAAGCGTGTGAGGAAGAAAGAG
PRIMER_LEFT_42=861,20
PRIMER_RIGHT_42=1683,22
PRIMER_LEFT_42_TM=58.497
PRIMER_RIGHT_42_TM=60.054
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=823
PRIMER_PAIR_43_PENALTY=3.572306
PRIMER_LEFT_43_PENALTY=1.683594
PRIMER_RIGHT_43_PENALTY=1.888711
PRIMER_LEFT_43_SEQUENCE=GGCGAACTGACAAACTGAATG
PRIMER_RIGHT_43_SEQUENCE=AAAGCGTGTGAGGAAGAAAGA
PRIMER_LEFT_43=862,21
PRIMER_RIGHT_43=1683,21
PRIMER_LEFT_43_TM=60.684
PRIMER_RIGHT_43_TM=59.111
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=822
PRIMER_PAIR_44_PENALTY=3.575739
PRIMER_LEFT_44_PENALTY=1.503187
PRIMER_RIGHT_44_PENALTY=2.072552
PRIMER_LEFT_44_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_44_SEQUENCE=AAAAAGCGTGTGAGGAAGAAAG
PRIMER_LEFT_44=861,20
PRIMER_RIGHT_44=1685,22
PRIMER_LEFT_44_TM=58.497
PRIMER_RIGHT_44_TM=59.927
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=825
PRIMER_PAIR_45_PENALTY=3.588715
PRIMER_LEFT_45_PENALTY=3.406057
PRIMER_RIGHT_45_PENALTY=0.182658
PRIMER_LEFT_45_SEQUENCE=TTAGGCGAACTGACAAACTG
PRIMER_RIGHT_45_SEQUENCE=ATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_45=859,20
PRIMER_RIGHT_45=1589,20
PRIMER_LEFT_45_TM=56.594
PRIMER_RIGHT_45_TM=60.183
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=11.2000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=731
PRIMER_PAIR_46_PENALTY=3.607923
PRIMER_LEFT_46_PENALTY=1.503187
PRIMER_RIGHT_46_PENALTY=2.104736
PRIMER_LEFT_46_SEQUENCE=AGGCGAACTGACAAACTGAA
PRIMER_RIGHT_46_SEQUENCE=ACAGACTCGTTCGGCGATTT
PRIMER_LEFT_46=861,20
PRIMER_RIGHT_46=1805,20
PRIMER_LEFT_46_TM=58.497
PRIMER_RIGHT_46_TM=62.105
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=945
PRIMER_PAIR_47_PENALTY=3.620035
PRIMER_LEFT_47_PENALTY=2.433726
PRIMER_RIGHT_47_PENALTY=1.186309
PRIMER_LEFT_47_SEQUENCE=TAGGCGAACTGACAAACTGAA
PRIMER_RIGHT_47_SEQUENCE=TATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_47=860,21
PRIMER_RIGHT_47=1590,21
PRIMER_LEFT_47_TM=58.566
PRIMER_RIGHT_47_TM=60.186
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=11.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=731
PRIMER_PAIR_48_PENALTY=3.620035
PRIMER_LEFT_48_PENALTY=2.433726
PRIMER_RIGHT_48_PENALTY=1.186309
PRIMER_LEFT_48_SEQUENCE=TTAGGCGAACTGACAAACTGA
PRIMER_RIGHT_48_SEQUENCE=TATAGGAGGAAGTGGTTGGGG
PRIMER_LEFT_48=859,21
PRIMER_RIGHT_48=1590,21
PRIMER_LEFT_48_TM=58.566
PRIMER_RIGHT_48_TM=60.186
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=11.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=732
PRIMER_PAIR_49_PENALTY=3.624286
PRIMER_LEFT_49_PENALTY=3.579454
PRIMER_RIGHT_49_PENALTY=0.044832
PRIMER_LEFT_49_SEQUENCE=AGGCGAACTGACAAACTGAATG
PRIMER_RIGHT_49_SEQUENCE=TCGGCGTCTGATTTCTCTTT
PRIMER_LEFT_49=861,22
PRIMER_RIGHT_49=1828,20
PRIMER_LEFT_49_TM=61.579
PRIMER_RIGHT_49_TM=59.955
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=968
=
SEQUENCE_ID=MAGIC.149_2_6716905_6716965_ler-0_hi-0_108193_11159_TRUE
SEQUENCE_TARGET=950,160
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNTNAGATCGTCCGGCTCGGCCCAAATGAGAGNTNCTCGGCCNAACAACNTCAAGTCGGCCCAGATGAAAGCTNCTCGGCCCAGCCNGACAAACTTGAGAGGGTAAAAANGTAAAANNGCAGGGGAGACCCTAGGTCAGCTCCTANCCTATAAATAGCAGAAGACACTATTGGGAANANGGACCANGAGTTATTACCTAGAGAATTTAAGAGAGAATAGTTGCCNANCTCTGTTATNTCTTGCTTTCCNGTTTTNCGTNNNNNNNNNNNNNNNNNNNNNNNTTGTACTTTNGGNNNNNNNNNNNNNNNNNNNNNTTCTCCGTTCTGTTAAATTACCGTCTTTTCCGTTCGTCATTAATAAAGAAGCTACTCCGTTCTCCCCAAACCCCTTAATAATTATTGTTACNACCTCNGTACNAACNNTTGGCGCCCACCGTGGGGNCANACAGNNTTTCTTCTNATATTGACNANCANATANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17473244_17473491_bur-0_edi-0_29072_27220_TRUE
SEQUENCE_TARGET=950,347
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCACNAAGCCACTAAATACCAAATCACAATTAATAAAAAGATAGNAAAAAAAGGAGAGAAAAAAGTTGTCCTCATCTCTCACAATTCTACTCCAGCTCGAAAGAGCGCTCGTCACTCAGATCTCCCATCTTCTCCGATATCCATAATCATTCCACTTTTCAATCTCCAATTTACATTACTTTATAANATTGAANTATAGAGATAACAGAAATGGCGATTAAACGTTGTTTTCTGCTACTTCNTCANCTCCAATAAGAGAGAGAGATTCTGTAGTTTTCTTCCATTGCTCTCTGTTCGAGATTATCTGACCAAAGTAATCTTCCTATACAAAATATTGGATNAAATATAATCAAAATTGTGGATCTTTGGGAAACATAAGCTTGAATNAAACACACATGCCAGCCTTTNTCTGCTTCTGGTTCGCTTNANCTTCANGTTTTCCCGCTCTGTNNNNNNNNNNNNNNCAAGGTACTTAATTTCTATCAACANCCATTANNTCTTTCTCANNAAANTTNAANNNTTTTTTATTTNCCNAGTGTTCTTAATTGTCCTTANNNNTATACATGTAACTGTANNNNTCTTTCTTTTTACTTCTGNTTTNTTTTTCTTTTATGTTTTATCTTGATGGGTNTCTCTTTCTNTCTCTTGGGTTTATATCAGTTAGAGTGGAGAGAAAGCAAAAGTTGAAGACAGANNGAGAGAGAGAGAGAANNGCGATGAACACAAGTGTTCGAGCTGTTCTTTCCTCCATGAAAGCTCCTTCAAAGCATGACACTACNNNNNNTCAAGTANCTTTCTTTNTCTCTCTCTCTNNTNNCANTTTTTCCACTTTTATTAACTCAATTTTGATTGCNTTTCTCTTTGGGGAAATCTTTCTTGGTGGATTATGCTTAGTCTNTGATAAAAAGTTTGTTCCTTTAGATTGATAAAAATTGGATTTCAGATATTTCTNAAGATTCTTGGCTTCAACATTGGACTATTTACCAACCGTATCTTTGCTNNNNNGTTTTGTTTTCTGAANAATAGTTATTCCATAGTGATGGTCACAGTGAAAGTGATCGATACTCAATTCTTATCCTCATTCTGGAAGTGTGTGTTGTTTTCCACTATCAAAGGCTCNCAACTATTGTTTCTTTATTANTAGCTCNAGTTCTGTTTCCTTCATTCTGTTNGTGAGTGTGTNTTTTTGGTCTATATGAGTAATGATGTTTCGTGGTATTCTGAAATCTGCAAAGGANGAGAAGAAGANGATGGAGTCTCAAGGCAATGGAGCTGTTGCTAANNCAGGAAAATCTCTGGTTAATCGNCGNCGAGCTAACAAAGANAAGAAAATGGATTTGCTACAAGATGTATGATGACACTTTCTTTGAGTTCATGATTTGNNNNNNNNGTGATNAGTTGNGTCTNAANNATGTNGTTGTTTGTATTAGGTTGATAAGCTTAAGAGGAAGCTGAGACAGGAGGAGAATGTNCATAGAGCANTGGAGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.144752
PRIMER_LEFT_0_PENALTY=0.047897
PRIMER_RIGHT_0_PENALTY=0.096855
PRIMER_LEFT_0_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_0_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_0=865,20
PRIMER_RIGHT_0=1640,20
PRIMER_LEFT_0_TM=59.952
PRIMER_RIGHT_0_TM=59.903
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=776
PRIMER_PAIR_1_PENALTY=0.711058
PRIMER_LEFT_1_PENALTY=0.047897
PRIMER_RIGHT_1_PENALTY=0.663161
PRIMER_LEFT_1_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_1_SEQUENCE=CCACCAAGAAAGATTTCCCC
PRIMER_LEFT_1=865,20
PRIMER_RIGHT_1=1641,20
PRIMER_LEFT_1_TM=59.952
PRIMER_RIGHT_1_TM=60.663
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=777
PRIMER_PAIR_2_PENALTY=0.800949
PRIMER_LEFT_2_PENALTY=0.047897
PRIMER_RIGHT_2_PENALTY=0.753052
PRIMER_LEFT_2_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_2_SEQUENCE=TCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_2=865,20
PRIMER_RIGHT_2=1734,20
PRIMER_LEFT_2_TM=59.952
PRIMER_RIGHT_2_TM=59.247
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=870
PRIMER_PAIR_3_PENALTY=1.122597
PRIMER_LEFT_3_PENALTY=0.047897
PRIMER_RIGHT_3_PENALTY=1.074700
PRIMER_LEFT_3_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_3_SEQUENCE=CCAAGAAAGATTTCCCCAAAG
PRIMER_LEFT_3=865,20
PRIMER_RIGHT_3=1638,21
PRIMER_LEFT_3_TM=59.952
PRIMER_RIGHT_3_TM=59.925
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=774
PRIMER_PAIR_4_PENALTY=1.151002
PRIMER_LEFT_4_PENALTY=0.047897
PRIMER_RIGHT_4_PENALTY=1.103105
PRIMER_LEFT_4_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_4_SEQUENCE=GTCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_4=865,20
PRIMER_RIGHT_4=1735,21
PRIMER_LEFT_4_TM=59.952
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=871
PRIMER_PAIR_5_PENALTY=1.185313
PRIMER_LEFT_5_PENALTY=1.088458
PRIMER_RIGHT_5_PENALTY=0.096855
PRIMER_LEFT_5_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_5_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_5=866,20
PRIMER_RIGHT_5=1640,20
PRIMER_LEFT_5_TM=58.912
PRIMER_RIGHT_5_TM=59.903
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=11.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=775
PRIMER_PAIR_6_PENALTY=1.312143
PRIMER_LEFT_6_PENALTY=1.215288
PRIMER_RIGHT_6_PENALTY=0.096855
PRIMER_LEFT_6_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_6_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_6=867,21
PRIMER_RIGHT_6=1640,20
PRIMER_LEFT_6_TM=60.215
PRIMER_RIGHT_6_TM=59.903
PRIMER_LEFT_6_GC_PERCENT=52.381
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=11.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=774
PRIMER_PAIR_7_PENALTY=1.570680
PRIMER_LEFT_7_PENALTY=0.047897
PRIMER_RIGHT_7_PENALTY=1.522783
PRIMER_LEFT_7_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_7_SEQUENCE=ACCAAGAAAGATTTCCCCAA
PRIMER_LEFT_7=865,20
PRIMER_RIGHT_7=1639,20
PRIMER_LEFT_7_TM=59.952
PRIMER_RIGHT_7_TM=58.477
PRIMER_LEFT_7_GC_PERCENT=60.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=10.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=775
PRIMER_PAIR_8_PENALTY=1.751618
PRIMER_LEFT_8_PENALTY=1.088458
PRIMER_RIGHT_8_PENALTY=0.663161
PRIMER_LEFT_8_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_8_SEQUENCE=CCACCAAGAAAGATTTCCCC
PRIMER_LEFT_8=866,20
PRIMER_RIGHT_8=1641,20
PRIMER_LEFT_8_TM=58.912
PRIMER_RIGHT_8_TM=60.663
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=10.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=776
PRIMER_PAIR_9_PENALTY=1.841510
PRIMER_LEFT_9_PENALTY=1.088458
PRIMER_RIGHT_9_PENALTY=0.753052
PRIMER_LEFT_9_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_9_SEQUENCE=TCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_9=866,20
PRIMER_RIGHT_9=1734,20
PRIMER_LEFT_9_TM=58.912
PRIMER_RIGHT_9_TM=59.247
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=869
PRIMER_PAIR_10_PENALTY=1.878449
PRIMER_LEFT_10_PENALTY=1.215288
PRIMER_RIGHT_10_PENALTY=0.663161
PRIMER_LEFT_10_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_10_SEQUENCE=CCACCAAGAAAGATTTCCCC
PRIMER_LEFT_10=867,21
PRIMER_RIGHT_10=1641,20
PRIMER_LEFT_10_TM=60.215
PRIMER_RIGHT_10_TM=60.663
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=10.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=775
PRIMER_PAIR_11_PENALTY=1.940403
PRIMER_LEFT_11_PENALTY=1.843548
PRIMER_RIGHT_11_PENALTY=0.096855
PRIMER_LEFT_11_SEQUENCE=CGTCACTCAGATCTCCCATC
PRIMER_RIGHT_11_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_11=868,20
PRIMER_RIGHT_11=1640,20
PRIMER_LEFT_11_TM=58.156
PRIMER_RIGHT_11_TM=59.903
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=11.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=773
PRIMER_PAIR_12_PENALTY=1.968340
PRIMER_LEFT_12_PENALTY=1.215288
PRIMER_RIGHT_12_PENALTY=0.753052
PRIMER_LEFT_12_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_12_SEQUENCE=TCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_12=867,21
PRIMER_RIGHT_12=1734,20
PRIMER_LEFT_12_TM=60.215
PRIMER_RIGHT_12_TM=59.247
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=868
PRIMER_PAIR_13_PENALTY=2.163157
PRIMER_LEFT_13_PENALTY=1.088458
PRIMER_RIGHT_13_PENALTY=1.074700
PRIMER_LEFT_13_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_13_SEQUENCE=CCAAGAAAGATTTCCCCAAAG
PRIMER_LEFT_13=866,20
PRIMER_RIGHT_13=1638,21
PRIMER_LEFT_13_TM=58.912
PRIMER_RIGHT_13_TM=59.925
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=773
PRIMER_PAIR_14_PENALTY=2.191562
PRIMER_LEFT_14_PENALTY=1.088458
PRIMER_RIGHT_14_PENALTY=1.103105
PRIMER_LEFT_14_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_14_SEQUENCE=GTCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_14=866,20
PRIMER_RIGHT_14=1735,21
PRIMER_LEFT_14_TM=58.912
PRIMER_RIGHT_14_TM=60.103
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=870
PRIMER_PAIR_15_PENALTY=2.231946
PRIMER_LEFT_15_PENALTY=0.047897
PRIMER_RIGHT_15_PENALTY=2.184049
PRIMER_LEFT_15_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_15_SEQUENCE=CACCAAGAAAGATTTCCCCAA
PRIMER_LEFT_15=865,20
PRIMER_RIGHT_15=1640,21
PRIMER_LEFT_15_TM=59.952
PRIMER_RIGHT_15_TM=61.184
PRIMER_LEFT_15_GC_PERCENT=60.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=10.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=776
PRIMER_PAIR_16_PENALTY=2.250894
PRIMER_LEFT_16_PENALTY=0.047897
PRIMER_RIGHT_16_PENALTY=2.202997
PRIMER_LEFT_16_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_16_SEQUENCE=AGTCCAATGTTGAAGCCAAG
PRIMER_LEFT_16=865,20
PRIMER_RIGHT_16=1736,20
PRIMER_LEFT_16_TM=59.952
PRIMER_RIGHT_16_TM=57.797
PRIMER_LEFT_16_GC_PERCENT=60.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=872
PRIMER_PAIR_17_PENALTY=2.289988
PRIMER_LEFT_17_PENALTY=1.215288
PRIMER_RIGHT_17_PENALTY=1.074700
PRIMER_LEFT_17_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_17_SEQUENCE=CCAAGAAAGATTTCCCCAAAG
PRIMER_LEFT_17=867,21
PRIMER_RIGHT_17=1638,21
PRIMER_LEFT_17_TM=60.215
PRIMER_RIGHT_17_TM=59.925
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=772
PRIMER_PAIR_18_PENALTY=2.318393
PRIMER_LEFT_18_PENALTY=1.215288
PRIMER_RIGHT_18_PENALTY=1.103105
PRIMER_LEFT_18_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_18_SEQUENCE=GTCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_18=867,21
PRIMER_RIGHT_18=1735,21
PRIMER_LEFT_18_TM=60.215
PRIMER_RIGHT_18_TM=60.103
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=869
PRIMER_PAIR_19_PENALTY=2.458637
PRIMER_LEFT_19_PENALTY=0.047897
PRIMER_RIGHT_19_PENALTY=2.410740
PRIMER_LEFT_19_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_19_SEQUENCE=AGGAAAGAACAGCTCGAACAC
PRIMER_LEFT_19=865,20
PRIMER_RIGHT_19=1505,21
PRIMER_LEFT_19_TM=59.952
PRIMER_RIGHT_19_TM=58.589
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=641
PRIMER_PAIR_20_PENALTY=2.471489
PRIMER_LEFT_20_PENALTY=0.047897
PRIMER_RIGHT_20_PENALTY=2.423592
PRIMER_LEFT_20_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_20_SEQUENCE=GAGGAAAGAACAGCTCGAACAC
PRIMER_LEFT_20=865,20
PRIMER_RIGHT_20=1506,22
PRIMER_LEFT_20_TM=59.952
PRIMER_RIGHT_20_TM=60.424
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=642
PRIMER_PAIR_21_PENALTY=2.506709
PRIMER_LEFT_21_PENALTY=1.843548
PRIMER_RIGHT_21_PENALTY=0.663161
PRIMER_LEFT_21_SEQUENCE=CGTCACTCAGATCTCCCATC
PRIMER_RIGHT_21_SEQUENCE=CCACCAAGAAAGATTTCCCC
PRIMER_LEFT_21=868,20
PRIMER_RIGHT_21=1641,20
PRIMER_LEFT_21_TM=58.156
PRIMER_RIGHT_21_TM=60.663
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=10.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=774
PRIMER_PAIR_22_PENALTY=2.509637
PRIMER_LEFT_22_PENALTY=0.047897
PRIMER_RIGHT_22_PENALTY=2.461740
PRIMER_LEFT_22_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_22_SEQUENCE=GGAAAACAACACACACTTCCAG
PRIMER_LEFT_22=865,20
PRIMER_RIGHT_22=1863,22
PRIMER_LEFT_22_TM=59.952
PRIMER_RIGHT_22_TM=59.538
PRIMER_LEFT_22_GC_PERCENT=60.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=999
PRIMER_PAIR_23_PENALTY=2.536228
PRIMER_LEFT_23_PENALTY=0.047897
PRIMER_RIGHT_23_PENALTY=2.488331
PRIMER_LEFT_23_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_23_SEQUENCE=GGAAAGAACAGCTCGAACAC
PRIMER_LEFT_23=865,20
PRIMER_RIGHT_23=1504,20
PRIMER_LEFT_23_TM=59.952
PRIMER_RIGHT_23_TM=57.512
PRIMER_LEFT_23_GC_PERCENT=60.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=640
PRIMER_PAIR_24_PENALTY=2.538055
PRIMER_LEFT_24_PENALTY=0.047897
PRIMER_RIGHT_24_PENALTY=2.490158
PRIMER_LEFT_24_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_24_SEQUENCE=GGAAAACAACACACACTTCCA
PRIMER_LEFT_24=865,20
PRIMER_RIGHT_24=1863,21
PRIMER_LEFT_24_TM=59.952
PRIMER_RIGHT_24_TM=58.510
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=999
PRIMER_PAIR_25_PENALTY=2.538055
PRIMER_LEFT_25_PENALTY=0.047897
PRIMER_RIGHT_25_PENALTY=2.490158
PRIMER_LEFT_25_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_25_SEQUENCE=TGGAAAACAACACACACTTCC
PRIMER_LEFT_25=865,20
PRIMER_RIGHT_25=1864,21
PRIMER_LEFT_25_TM=59.952
PRIMER_RIGHT_25_TM=58.510
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=1000
PRIMER_PAIR_26_PENALTY=2.596600
PRIMER_LEFT_26_PENALTY=1.843548
PRIMER_RIGHT_26_PENALTY=0.753052
PRIMER_LEFT_26_SEQUENCE=CGTCACTCAGATCTCCCATC
PRIMER_RIGHT_26_SEQUENCE=TCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_26=868,20
PRIMER_RIGHT_26=1734,20
PRIMER_LEFT_26_TM=58.156
PRIMER_RIGHT_26_TM=59.247
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.1000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=867
PRIMER_PAIR_27_PENALTY=2.611241
PRIMER_LEFT_27_PENALTY=1.088458
PRIMER_RIGHT_27_PENALTY=1.522783
PRIMER_LEFT_27_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_27_SEQUENCE=ACCAAGAAAGATTTCCCCAA
PRIMER_LEFT_27=866,20
PRIMER_RIGHT_27=1639,20
PRIMER_LEFT_27_TM=58.912
PRIMER_RIGHT_27_TM=58.477
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=10.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=774
PRIMER_PAIR_28_PENALTY=2.714492
PRIMER_LEFT_28_PENALTY=0.047897
PRIMER_RIGHT_28_PENALTY=2.666595
PRIMER_LEFT_28_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_28_SEQUENCE=ACCAAGAAAGATTTCCCCAAAG
PRIMER_LEFT_28=865,20
PRIMER_RIGHT_28=1639,22
PRIMER_LEFT_28_TM=59.952
PRIMER_RIGHT_28_TM=60.667
PRIMER_LEFT_28_GC_PERCENT=60.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=775
PRIMER_PAIR_29_PENALTY=2.738071
PRIMER_LEFT_29_PENALTY=1.215288
PRIMER_RIGHT_29_PENALTY=1.522783
PRIMER_LEFT_29_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_29_SEQUENCE=ACCAAGAAAGATTTCCCCAA
PRIMER_LEFT_29=867,21
PRIMER_RIGHT_29=1639,20
PRIMER_LEFT_29_TM=60.215
PRIMER_RIGHT_29_TM=58.477
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=10.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=773
PRIMER_PAIR_30_PENALTY=2.745931
PRIMER_LEFT_30_PENALTY=2.649076
PRIMER_RIGHT_30_PENALTY=0.096855
PRIMER_LEFT_30_SEQUENCE=CGTCACTCAGATCTCCCATCTT
PRIMER_RIGHT_30_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_30=868,22
PRIMER_RIGHT_30=1640,20
PRIMER_LEFT_30_TM=60.649
PRIMER_RIGHT_30_TM=59.903
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=11.2000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=773
PRIMER_PAIR_31_PENALTY=2.809759
PRIMER_LEFT_31_PENALTY=0.047897
PRIMER_RIGHT_31_PENALTY=2.761862
PRIMER_LEFT_31_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_31_SEQUENCE=CAAGAAAGATTTCCCCAAAGAG
PRIMER_LEFT_31=865,20
PRIMER_RIGHT_31=1637,22
PRIMER_LEFT_31_TM=59.952
PRIMER_RIGHT_31_TM=59.238
PRIMER_LEFT_31_GC_PERCENT=60.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=773
PRIMER_PAIR_32_PENALTY=2.918248
PRIMER_LEFT_32_PENALTY=1.843548
PRIMER_RIGHT_32_PENALTY=1.074700
PRIMER_LEFT_32_SEQUENCE=CGTCACTCAGATCTCCCATC
PRIMER_RIGHT_32_SEQUENCE=CCAAGAAAGATTTCCCCAAAG
PRIMER_LEFT_32=868,20
PRIMER_RIGHT_32=1638,21
PRIMER_LEFT_32_TM=58.156
PRIMER_RIGHT_32_TM=59.925
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=771
PRIMER_PAIR_33_PENALTY=2.946653
PRIMER_LEFT_33_PENALTY=1.843548
PRIMER_RIGHT_33_PENALTY=1.103105
PRIMER_LEFT_33_SEQUENCE=CGTCACTCAGATCTCCCATC
PRIMER_RIGHT_33_SEQUENCE=GTCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_33=868,20
PRIMER_RIGHT_33=1735,21
PRIMER_LEFT_33_TM=58.156
PRIMER_RIGHT_33_TM=60.103
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=868
PRIMER_PAIR_34_PENALTY=3.062404
PRIMER_LEFT_34_PENALTY=0.047897
PRIMER_RIGHT_34_PENALTY=3.014506
PRIMER_LEFT_34_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_34_SEQUENCE=AGTCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_34=865,20
PRIMER_RIGHT_34=1736,22
PRIMER_LEFT_34_TM=59.952
PRIMER_RIGHT_34_TM=61.015
PRIMER_LEFT_34_GC_PERCENT=60.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=872
PRIMER_PAIR_35_PENALTY=3.155268
PRIMER_LEFT_35_PENALTY=0.047897
PRIMER_RIGHT_35_PENALTY=3.107370
PRIMER_LEFT_35_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_35_SEQUENCE=TAGTCCAATGTTGAAGCCAAG
PRIMER_LEFT_35=865,20
PRIMER_RIGHT_35=1737,21
PRIMER_LEFT_35_TM=59.952
PRIMER_RIGHT_35_TM=57.893
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=873
PRIMER_PAIR_36_PENALTY=3.243739
PRIMER_LEFT_36_PENALTY=3.146884
PRIMER_RIGHT_36_PENALTY=0.096855
PRIMER_LEFT_36_SEQUENCE=CTCAGATCTCCCATCTTCTCC
PRIMER_RIGHT_36_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_36=873,21
PRIMER_RIGHT_36=1640,20
PRIMER_LEFT_36_TM=57.853
PRIMER_RIGHT_36_TM=59.903
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=11.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=768
PRIMER_PAIR_37_PENALTY=3.265060
PRIMER_LEFT_37_PENALTY=3.168205
PRIMER_RIGHT_37_PENALTY=0.096855
PRIMER_LEFT_37_SEQUENCE=TCACTCAGATCTCCCATCTTCTC
PRIMER_RIGHT_37_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_37=870,23
PRIMER_RIGHT_37=1640,20
PRIMER_LEFT_37_TM=59.832
PRIMER_RIGHT_37_TM=59.903
PRIMER_LEFT_37_GC_PERCENT=47.826
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=11.2000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=771
PRIMER_PAIR_38_PENALTY=3.272506
PRIMER_LEFT_38_PENALTY=1.088458
PRIMER_RIGHT_38_PENALTY=2.184049
PRIMER_LEFT_38_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_38_SEQUENCE=CACCAAGAAAGATTTCCCCAA
PRIMER_LEFT_38=866,20
PRIMER_RIGHT_38=1640,21
PRIMER_LEFT_38_TM=58.912
PRIMER_RIGHT_38_TM=61.184
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=10.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=775
PRIMER_PAIR_39_PENALTY=3.288767
PRIMER_LEFT_39_PENALTY=3.191911
PRIMER_RIGHT_39_PENALTY=0.096855
PRIMER_LEFT_39_SEQUENCE=CTCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_39_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_39=866,22
PRIMER_RIGHT_39=1640,20
PRIMER_LEFT_39_TM=61.192
PRIMER_RIGHT_39_TM=59.903
PRIMER_LEFT_39_GC_PERCENT=54.545
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.1000
PRIMER_RIGHT_39_END_STABILITY=11.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=775
PRIMER_PAIR_40_PENALTY=3.291454
PRIMER_LEFT_40_PENALTY=1.088458
PRIMER_RIGHT_40_PENALTY=2.202997
PRIMER_LEFT_40_SEQUENCE=CTCGTCACTCAGATCTCCCA
PRIMER_RIGHT_40_SEQUENCE=AGTCCAATGTTGAAGCCAAG
PRIMER_LEFT_40=866,20
PRIMER_RIGHT_40=1736,20
PRIMER_LEFT_40_TM=58.912
PRIMER_RIGHT_40_TM=57.797
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=871
PRIMER_PAIR_41_PENALTY=3.312236
PRIMER_LEFT_41_PENALTY=2.649076
PRIMER_RIGHT_41_PENALTY=0.663161
PRIMER_LEFT_41_SEQUENCE=CGTCACTCAGATCTCCCATCTT
PRIMER_RIGHT_41_SEQUENCE=CCACCAAGAAAGATTTCCCC
PRIMER_LEFT_41=868,22
PRIMER_RIGHT_41=1641,20
PRIMER_LEFT_41_TM=60.649
PRIMER_RIGHT_41_TM=60.663
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.6000
PRIMER_RIGHT_41_END_STABILITY=10.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=774
PRIMER_PAIR_42_PENALTY=3.334938
PRIMER_LEFT_42_PENALTY=3.238083
PRIMER_RIGHT_42_PENALTY=0.096855
PRIMER_LEFT_42_SEQUENCE=ACTCAGATCTCCCATCTTCTCC
PRIMER_RIGHT_42_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_42=872,22
PRIMER_RIGHT_42=1640,20
PRIMER_LEFT_42_TM=58.762
PRIMER_RIGHT_42_TM=59.903
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=11.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=769
PRIMER_PAIR_43_PENALTY=3.366331
PRIMER_LEFT_43_PENALTY=1.843548
PRIMER_RIGHT_43_PENALTY=1.522783
PRIMER_LEFT_43_SEQUENCE=CGTCACTCAGATCTCCCATC
PRIMER_RIGHT_43_SEQUENCE=ACCAAGAAAGATTTCCCCAA
PRIMER_LEFT_43=868,20
PRIMER_RIGHT_43=1639,20
PRIMER_LEFT_43_TM=58.156
PRIMER_RIGHT_43_TM=58.477
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=10.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=772
PRIMER_PAIR_44_PENALTY=3.399337
PRIMER_LEFT_44_PENALTY=1.215288
PRIMER_RIGHT_44_PENALTY=2.184049
PRIMER_LEFT_44_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_44_SEQUENCE=CACCAAGAAAGATTTCCCCAA
PRIMER_LEFT_44=867,21
PRIMER_RIGHT_44=1640,21
PRIMER_LEFT_44_TM=60.215
PRIMER_RIGHT_44_TM=61.184
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=10.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=774
PRIMER_PAIR_45_PENALTY=3.402128
PRIMER_LEFT_45_PENALTY=2.649076
PRIMER_RIGHT_45_PENALTY=0.753052
PRIMER_LEFT_45_SEQUENCE=CGTCACTCAGATCTCCCATCTT
PRIMER_RIGHT_45_SEQUENCE=TCCAATGTTGAAGCCAAGAA
PRIMER_LEFT_45=868,22
PRIMER_RIGHT_45=1734,20
PRIMER_LEFT_45_TM=60.649
PRIMER_RIGHT_45_TM=59.247
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.6000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=867
PRIMER_PAIR_46_PENALTY=3.402564
PRIMER_LEFT_46_PENALTY=3.305709
PRIMER_RIGHT_46_PENALTY=0.096855
PRIMER_LEFT_46_SEQUENCE=TCAGATCTCCCATCTTCTCC
PRIMER_RIGHT_46_SEQUENCE=CACCAAGAAAGATTTCCCCA
PRIMER_LEFT_46=874,20
PRIMER_RIGHT_46=1640,20
PRIMER_LEFT_46_TM=56.694
PRIMER_RIGHT_46_TM=59.903
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=11.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=767
PRIMER_PAIR_47_PENALTY=3.418285
PRIMER_LEFT_47_PENALTY=1.215288
PRIMER_RIGHT_47_PENALTY=2.202997
PRIMER_LEFT_47_SEQUENCE=TCGTCACTCAGATCTCCCATC
PRIMER_RIGHT_47_SEQUENCE=AGTCCAATGTTGAAGCCAAG
PRIMER_LEFT_47=867,21
PRIMER_RIGHT_47=1736,20
PRIMER_LEFT_47_TM=60.215
PRIMER_RIGHT_47_TM=57.797
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=870
PRIMER_PAIR_48_PENALTY=3.420627
PRIMER_LEFT_48_PENALTY=0.047897
PRIMER_RIGHT_48_PENALTY=3.372729
PRIMER_LEFT_48_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_48_SEQUENCE=TCAACTTTTGCTTTCTCTCCAC
PRIMER_LEFT_48=865,20
PRIMER_RIGHT_48=1445,22
PRIMER_LEFT_48_TM=59.952
PRIMER_RIGHT_48_TM=58.627
PRIMER_LEFT_48_GC_PERCENT=60.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=581
PRIMER_PAIR_49_PENALTY=3.435963
PRIMER_LEFT_49_PENALTY=0.047897
PRIMER_RIGHT_49_PENALTY=3.388066
PRIMER_LEFT_49_SEQUENCE=GCTCGTCACTCAGATCTCCC
PRIMER_RIGHT_49_SEQUENCE=TGGAAAACAACACACACTTCCA
PRIMER_LEFT_49=865,20
PRIMER_RIGHT_49=1864,22
PRIMER_LEFT_49_TM=59.952
PRIMER_RIGHT_49_TM=61.388
PRIMER_LEFT_49_GC_PERCENT=60.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=1000
=
SEQUENCE_ID=MAGIC.426_2_1528324_1528657_oy-0_rsch-4_149110_27330_TRUE
SEQUENCE_TARGET=950,433
SEQUENCE_TEMPLATE=NAAATCAATTTGAGAAGACTANTGGATAATNGTGAACACGTTTACATGACTTTTGACTTGATAGTTTGCTATTCTTGTTGTATCTGTGTGAATTGTTATAGGTTGCTNGATTTTGATGTTCATGTGTCAAANTTGATAATGTAGCTTTTGTATCTGATTAATTAAGATTCTAATGCAGTTGAGTTTTTGGTCTTAAGATTANGCATTGTATATTATACCAACTTGTGAAGNGGTTCTGTTGTTTTCTGAATTTCTCTTATTTTGTGTTTCATTCANCAAAAGTGTGTCGTGAGTTNATATTCTTTTAGGCTGAATATAAATGTATGTATAAAATTTGGCTTATATTGTTTGATAAGAACACTAGACCTCTTTCAAGTTGAGCGTTATGTTGATGTATATTACTTGACTCATTTTCAATTCTCTTGACAATATTTGACATTGCATTTACATGTGGTTCCTGTGTTTGTTTAGTGTTCAACCTGAATGTTGAAGTTTAAGACANAAATCATCTATACTTGGTTGTTTCTTGATTTTGAATCCTTNNNAAGACATGGNATTTCTCNTCTCATCAGGTAAGTNACTGAAAGATGGGGCGTGTTAGAACCAAGACCGTGAAGAAATCTTCTCGTCAAGTCATTGAGAAGTACTACTCTCGCATGACTCTTGACTTTCACACTAACAAGAAGATCCTTGAAGAGGTTGCCATCATCCCATCAAAGAGACTCCGCAACAAGATTGCTGGATTCTCCACCCACTTGATGAAACGTATCCAGAAGGGACCAGTCCGTGGAATCTCACTCAAGCTTCAAGAAGAAGAGCGTGAACGCCGTATGGACTTTGTTCCCGATGAGTCTGCTATCAAGACTGATGAGATCAAGGTCGACAAAGAGACTCTTGAGATGCTTGCTTCTCTAGGAATGTCTGACANTCNCGGCATCTCTGCAGTCGACCCACAACAAGCTATGGCACCAATCNCTGCTTTNNNCGGCGGCAGGGCACCNAGAAGATACTAAGANGGACGATTGGCTCTCTTTTTTCACNGTTAGGGACAAGAAAGACTTGTGTGGTTGTTTATTGTTTCTTTTGATTATGTCTTAAANTCAATGTGAGACTCTTTCGTATTAATGGNNNTTGAGTTATGTGGAACCAAAATTGGAGTTCNAACATTGATGANAGTCTCTNNNNNTATATCCTAGNNTTTGCAACCTGTTAATCCAGGAGGTGACGGAAAACACCAGAATGAAATGTGTCCCAACTTTGATGATANTNTCTTATCATACATCCTAGTTTTTGGCACATAAGATTATAAAACAAAATNGTAAGAGATTCTTGTNGTAAGCAGAAANGTGGGAAGTANTTANNGAAAGAAACCTCAAGAAGTACTAACCTCATGATCTTTTCAACTAAACGATCATCCTGAGNGATATGATGAACCTTTGTAGAGCTGGTAACGTTGATTACCCAAGATCCTCTATCAACTTCACAGAGTAAATGGTAAAGTTTCACCCAATCACCAGGTTTAACCCGATAAAAGAGATGACTGCTTAGATGAACTCTCAAGATCGTTCCAACGGTTGGAAAGCTGAGCAGTACATCTCTTGACAGNAAAGTATGAACAGAGAGACTGCTAAACATTGTACACCAAATAGAAGAAGAGTAAGNGTAGGATATGGAAAGCGTAGAGTCAAGCNNNNNNNNNNNNNNNNNNNCAAAACAGATAATCTGAAGATTNAAAACTCNTTTCCTTGCTAGTTAATANTCACTTAGCCAGAATAGATGCTGGGGGTGCATCAGTCCCGTCCCAAACAAAAACAGCGCTCGTATCTTCATCGGCATGGAGAATCTCCATCACAANAAACAGCAGAAAACAAGGTAGNATCAGTCAACAAAACATAAAAGGTATACAGTTTGAGCCAAAAAAAGATTTGTAAGTAACAATGAAGTGCTTTCTCATGTTAATAGAGAGTATAGAGCATATTACCCTACACACCAAATCAAAGCGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACTCTGCATAGCTAAGTACCAAAACAGGAAATGAAAGCTTATTGGAACTACATTGGTAACAAACNAAATCCTCACCTTNGAANAAGGCANAGGAAGAAACCCATCNACTACAAGCAGCNTTTACTCTTTTTCCACTATCAAAAGTTTGCATCTACACAATAAAAATCCAAACTTTATNACCTCCTNCACAAAACACACAANGAAAATGANGANTCTTTGTNCCTTAATGCGAGTAAACAGAATCATGTCGTCGTGGTTCTTGATTTGAGGAAGTTGTTCAAGAGTCCTACT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.179711
PRIMER_LEFT_0_PENALTY=0.000037
PRIMER_RIGHT_0_PENALTY=0.179674
PRIMER_LEFT_0_SEQUENCE=ACGCCGTATGGACTTTGTTC
PRIMER_RIGHT_0_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_0=823,20
PRIMER_RIGHT_0=1517,20
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=59.820
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=695
PRIMER_PAIR_1_PENALTY=0.179711
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.179674
PRIMER_LEFT_1_SEQUENCE=GAACGCCGTATGGACTTTGT
PRIMER_RIGHT_1_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_1=821,20
PRIMER_RIGHT_1=1517,20
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=697
PRIMER_PAIR_2_PENALTY=0.209906
PRIMER_LEFT_2_PENALTY=0.000037
PRIMER_RIGHT_2_PENALTY=0.209870
PRIMER_LEFT_2_SEQUENCE=ACGCCGTATGGACTTTGTTC
PRIMER_RIGHT_2_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_2=823,20
PRIMER_RIGHT_2=1519,20
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=60.210
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=697
PRIMER_PAIR_3_PENALTY=0.209906
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.209870
PRIMER_LEFT_3_SEQUENCE=ACGCCGTATGGACTTTGTTC
PRIMER_RIGHT_3_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_3=823,20
PRIMER_RIGHT_3=1520,20
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.210
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=698
PRIMER_PAIR_4_PENALTY=0.209906
PRIMER_LEFT_4_PENALTY=0.000037
PRIMER_RIGHT_4_PENALTY=0.209870
PRIMER_LEFT_4_SEQUENCE=GAACGCCGTATGGACTTTGT
PRIMER_RIGHT_4_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_4=821,20
PRIMER_RIGHT_4=1519,20
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=60.210
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=699
PRIMER_PAIR_5_PENALTY=0.209906
PRIMER_LEFT_5_PENALTY=0.000037
PRIMER_RIGHT_5_PENALTY=0.209870
PRIMER_LEFT_5_SEQUENCE=GAACGCCGTATGGACTTTGT
PRIMER_RIGHT_5_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_5=821,20
PRIMER_RIGHT_5=1520,20
PRIMER_LEFT_5_TM=60.000
PRIMER_RIGHT_5_TM=60.210
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=700
PRIMER_PAIR_6_PENALTY=0.253288
PRIMER_LEFT_6_PENALTY=0.073614
PRIMER_RIGHT_6_PENALTY=0.179674
PRIMER_LEFT_6_SEQUENCE=GAAAGATGGGGCGTGTTAGA
PRIMER_RIGHT_6_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_6=582,20
PRIMER_RIGHT_6=1517,20
PRIMER_LEFT_6_TM=60.074
PRIMER_RIGHT_6_TM=59.820
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.0000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=936
PRIMER_PAIR_7_PENALTY=0.283484
PRIMER_LEFT_7_PENALTY=0.073614
PRIMER_RIGHT_7_PENALTY=0.209870
PRIMER_LEFT_7_SEQUENCE=GAAAGATGGGGCGTGTTAGA
PRIMER_RIGHT_7_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_7=582,20
PRIMER_RIGHT_7=1519,20
PRIMER_LEFT_7_TM=60.074
PRIMER_RIGHT_7_TM=60.210
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.0000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=938
PRIMER_PAIR_8_PENALTY=0.283484
PRIMER_LEFT_8_PENALTY=0.073614
PRIMER_RIGHT_8_PENALTY=0.209870
PRIMER_LEFT_8_SEQUENCE=GAAAGATGGGGCGTGTTAGA
PRIMER_RIGHT_8_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_8=582,20
PRIMER_RIGHT_8=1520,20
PRIMER_LEFT_8_TM=60.074
PRIMER_RIGHT_8_TM=60.210
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=939
PRIMER_PAIR_9_PENALTY=0.320941
PRIMER_LEFT_9_PENALTY=0.000037
PRIMER_RIGHT_9_PENALTY=0.320904
PRIMER_LEFT_9_SEQUENCE=ACGCCGTATGGACTTTGTTC
PRIMER_RIGHT_9_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_9=823,20
PRIMER_RIGHT_9=1518,20
PRIMER_LEFT_9_TM=60.000
PRIMER_RIGHT_9_TM=59.679
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=696
PRIMER_PAIR_10_PENALTY=0.320941
PRIMER_LEFT_10_PENALTY=0.000037
PRIMER_RIGHT_10_PENALTY=0.320904
PRIMER_LEFT_10_SEQUENCE=GAACGCCGTATGGACTTTGT
PRIMER_RIGHT_10_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_10=821,20
PRIMER_RIGHT_10=1518,20
PRIMER_LEFT_10_TM=60.000
PRIMER_RIGHT_10_TM=59.679
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=698
PRIMER_PAIR_11_PENALTY=0.394518
PRIMER_LEFT_11_PENALTY=0.073614
PRIMER_RIGHT_11_PENALTY=0.320904
PRIMER_LEFT_11_SEQUENCE=GAAAGATGGGGCGTGTTAGA
PRIMER_RIGHT_11_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_11=582,20
PRIMER_RIGHT_11=1518,20
PRIMER_LEFT_11_TM=60.074
PRIMER_RIGHT_11_TM=59.679
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.0000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=937
PRIMER_PAIR_12_PENALTY=0.497033
PRIMER_LEFT_12_PENALTY=0.317359
PRIMER_RIGHT_12_PENALTY=0.179674
PRIMER_LEFT_12_SEQUENCE=ATGGACTTTGTTCCCGATGA
PRIMER_RIGHT_12_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_12=830,20
PRIMER_RIGHT_12=1517,20
PRIMER_LEFT_12_TM=60.317
PRIMER_RIGHT_12_TM=59.820
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=688
PRIMER_PAIR_13_PENALTY=0.527228
PRIMER_LEFT_13_PENALTY=0.317359
PRIMER_RIGHT_13_PENALTY=0.209870
PRIMER_LEFT_13_SEQUENCE=ATGGACTTTGTTCCCGATGA
PRIMER_RIGHT_13_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_13=830,20
PRIMER_RIGHT_13=1519,20
PRIMER_LEFT_13_TM=60.317
PRIMER_RIGHT_13_TM=60.210
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=690
PRIMER_PAIR_14_PENALTY=0.527228
PRIMER_LEFT_14_PENALTY=0.317359
PRIMER_RIGHT_14_PENALTY=0.209870
PRIMER_LEFT_14_SEQUENCE=ATGGACTTTGTTCCCGATGA
PRIMER_RIGHT_14_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_14=830,20
PRIMER_RIGHT_14=1520,20
PRIMER_LEFT_14_TM=60.317
PRIMER_RIGHT_14_TM=60.210
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=691
PRIMER_PAIR_15_PENALTY=0.605596
PRIMER_LEFT_15_PENALTY=0.425922
PRIMER_RIGHT_15_PENALTY=0.179674
PRIMER_LEFT_15_SEQUENCE=GCCATCATCCCATCAAAGAG
PRIMER_RIGHT_15_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_15=701,20
PRIMER_RIGHT_15=1517,20
PRIMER_LEFT_15_TM=60.426
PRIMER_RIGHT_15_TM=59.820
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=817
PRIMER_PAIR_16_PENALTY=0.613815
PRIMER_LEFT_16_PENALTY=0.434141
PRIMER_RIGHT_16_PENALTY=0.179674
PRIMER_LEFT_16_SEQUENCE=TCAAAGAGACTCCGCAACAA
PRIMER_RIGHT_16_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_16=713,20
PRIMER_RIGHT_16=1517,20
PRIMER_LEFT_16_TM=59.566
PRIMER_RIGHT_16_TM=59.820
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=805
PRIMER_PAIR_17_PENALTY=0.635791
PRIMER_LEFT_17_PENALTY=0.425922
PRIMER_RIGHT_17_PENALTY=0.209870
PRIMER_LEFT_17_SEQUENCE=GCCATCATCCCATCAAAGAG
PRIMER_RIGHT_17_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_17=701,20
PRIMER_RIGHT_17=1519,20
PRIMER_LEFT_17_TM=60.426
PRIMER_RIGHT_17_TM=60.210
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=819
PRIMER_PAIR_18_PENALTY=0.638263
PRIMER_LEFT_18_PENALTY=0.317359
PRIMER_RIGHT_18_PENALTY=0.320904
PRIMER_LEFT_18_SEQUENCE=ATGGACTTTGTTCCCGATGA
PRIMER_RIGHT_18_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_18=830,20
PRIMER_RIGHT_18=1518,20
PRIMER_LEFT_18_TM=60.317
PRIMER_RIGHT_18_TM=59.679
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=689
PRIMER_PAIR_19_PENALTY=0.644010
PRIMER_LEFT_19_PENALTY=0.434141
PRIMER_RIGHT_19_PENALTY=0.209870
PRIMER_LEFT_19_SEQUENCE=TCAAAGAGACTCCGCAACAA
PRIMER_RIGHT_19_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_19=713,20
PRIMER_RIGHT_19=1519,20
PRIMER_LEFT_19_TM=59.566
PRIMER_RIGHT_19_TM=60.210
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=807
PRIMER_PAIR_20_PENALTY=0.644010
PRIMER_LEFT_20_PENALTY=0.434141
PRIMER_RIGHT_20_PENALTY=0.209870
PRIMER_LEFT_20_SEQUENCE=TCAAAGAGACTCCGCAACAA
PRIMER_RIGHT_20_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_20=713,20
PRIMER_RIGHT_20=1520,20
PRIMER_LEFT_20_TM=59.566
PRIMER_RIGHT_20_TM=60.210
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=808
PRIMER_PAIR_21_PENALTY=0.680899
PRIMER_LEFT_21_PENALTY=0.501224
PRIMER_RIGHT_21_PENALTY=0.179674
PRIMER_LEFT_21_SEQUENCE=AACGCCGTATGGACTTTGTT
PRIMER_RIGHT_21_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_21=822,20
PRIMER_RIGHT_21=1517,20
PRIMER_LEFT_21_TM=59.499
PRIMER_RIGHT_21_TM=59.820
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=696
PRIMER_PAIR_22_PENALTY=0.711094
PRIMER_LEFT_22_PENALTY=0.501224
PRIMER_RIGHT_22_PENALTY=0.209870
PRIMER_LEFT_22_SEQUENCE=AACGCCGTATGGACTTTGTT
PRIMER_RIGHT_22_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_22=822,20
PRIMER_RIGHT_22=1519,20
PRIMER_LEFT_22_TM=59.499
PRIMER_RIGHT_22_TM=60.210
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=698
PRIMER_PAIR_23_PENALTY=0.711094
PRIMER_LEFT_23_PENALTY=0.501224
PRIMER_RIGHT_23_PENALTY=0.209870
PRIMER_LEFT_23_SEQUENCE=AACGCCGTATGGACTTTGTT
PRIMER_RIGHT_23_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_23=822,20
PRIMER_RIGHT_23=1520,20
PRIMER_LEFT_23_TM=59.499
PRIMER_RIGHT_23_TM=60.210
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=699
PRIMER_PAIR_24_PENALTY=0.746826
PRIMER_LEFT_24_PENALTY=0.425922
PRIMER_RIGHT_24_PENALTY=0.320904
PRIMER_LEFT_24_SEQUENCE=GCCATCATCCCATCAAAGAG
PRIMER_RIGHT_24_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_24=701,20
PRIMER_RIGHT_24=1518,20
PRIMER_LEFT_24_TM=60.426
PRIMER_RIGHT_24_TM=59.679
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=7.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=818
PRIMER_PAIR_25_PENALTY=0.755045
PRIMER_LEFT_25_PENALTY=0.434141
PRIMER_RIGHT_25_PENALTY=0.320904
PRIMER_LEFT_25_SEQUENCE=TCAAAGAGACTCCGCAACAA
PRIMER_RIGHT_25_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_25=713,20
PRIMER_RIGHT_25=1518,20
PRIMER_LEFT_25_TM=59.566
PRIMER_RIGHT_25_TM=59.679
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=806
PRIMER_PAIR_26_PENALTY=0.774387
PRIMER_LEFT_26_PENALTY=0.594713
PRIMER_RIGHT_26_PENALTY=0.179674
PRIMER_LEFT_26_SEQUENCE=AACGTATCCAGAAGGGACCA
PRIMER_RIGHT_26_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_26=762,20
PRIMER_RIGHT_26=1517,20
PRIMER_LEFT_26_TM=59.405
PRIMER_RIGHT_26_TM=59.820
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=756
PRIMER_PAIR_27_PENALTY=0.804583
PRIMER_LEFT_27_PENALTY=0.594713
PRIMER_RIGHT_27_PENALTY=0.209870
PRIMER_LEFT_27_SEQUENCE=AACGTATCCAGAAGGGACCA
PRIMER_RIGHT_27_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_27=762,20
PRIMER_RIGHT_27=1519,20
PRIMER_LEFT_27_TM=59.405
PRIMER_RIGHT_27_TM=60.210
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=758
PRIMER_PAIR_28_PENALTY=0.804583
PRIMER_LEFT_28_PENALTY=0.594713
PRIMER_RIGHT_28_PENALTY=0.209870
PRIMER_LEFT_28_SEQUENCE=AACGTATCCAGAAGGGACCA
PRIMER_RIGHT_28_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_28=762,20
PRIMER_RIGHT_28=1520,20
PRIMER_LEFT_28_TM=59.405
PRIMER_RIGHT_28_TM=60.210
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=759
PRIMER_PAIR_29_PENALTY=0.822128
PRIMER_LEFT_29_PENALTY=0.501224
PRIMER_RIGHT_29_PENALTY=0.320904
PRIMER_LEFT_29_SEQUENCE=AACGCCGTATGGACTTTGTT
PRIMER_RIGHT_29_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_29=822,20
PRIMER_RIGHT_29=1518,20
PRIMER_LEFT_29_TM=59.499
PRIMER_RIGHT_29_TM=59.679
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=697
PRIMER_PAIR_30_PENALTY=0.915617
PRIMER_LEFT_30_PENALTY=0.594713
PRIMER_RIGHT_30_PENALTY=0.320904
PRIMER_LEFT_30_SEQUENCE=AACGTATCCAGAAGGGACCA
PRIMER_RIGHT_30_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_30=762,20
PRIMER_RIGHT_30=1518,20
PRIMER_LEFT_30_TM=59.405
PRIMER_RIGHT_30_TM=59.679
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=757
PRIMER_PAIR_31_PENALTY=0.980866
PRIMER_LEFT_31_PENALTY=0.801192
PRIMER_RIGHT_31_PENALTY=0.179674
PRIMER_LEFT_31_SEQUENCE=TTGTTCCCGATGAGTCTGCT
PRIMER_RIGHT_31_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_31=837,20
PRIMER_RIGHT_31=1517,20
PRIMER_LEFT_31_TM=60.801
PRIMER_RIGHT_31_TM=59.820
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=681
PRIMER_PAIR_32_PENALTY=1.011061
PRIMER_LEFT_32_PENALTY=0.801192
PRIMER_RIGHT_32_PENALTY=0.209870
PRIMER_LEFT_32_SEQUENCE=TTGTTCCCGATGAGTCTGCT
PRIMER_RIGHT_32_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_32=837,20
PRIMER_RIGHT_32=1519,20
PRIMER_LEFT_32_TM=60.801
PRIMER_RIGHT_32_TM=60.210
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=683
PRIMER_PAIR_33_PENALTY=1.011061
PRIMER_LEFT_33_PENALTY=0.801192
PRIMER_RIGHT_33_PENALTY=0.209870
PRIMER_LEFT_33_SEQUENCE=TTGTTCCCGATGAGTCTGCT
PRIMER_RIGHT_33_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_33=837,20
PRIMER_RIGHT_33=1520,20
PRIMER_LEFT_33_TM=60.801
PRIMER_RIGHT_33_TM=60.210
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=684
PRIMER_PAIR_34_PENALTY=1.122096
PRIMER_LEFT_34_PENALTY=0.801192
PRIMER_RIGHT_34_PENALTY=0.320904
PRIMER_LEFT_34_SEQUENCE=TTGTTCCCGATGAGTCTGCT
PRIMER_RIGHT_34_SEQUENCE=ACCTGGTGATTGGGTGAAAC
PRIMER_LEFT_34=837,20
PRIMER_RIGHT_34=1518,20
PRIMER_LEFT_34_TM=60.801
PRIMER_RIGHT_34_TM=59.679
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=682
PRIMER_PAIR_35_PENALTY=1.165219
PRIMER_LEFT_35_PENALTY=0.985544
PRIMER_RIGHT_35_PENALTY=0.179674
PRIMER_LEFT_35_SEQUENCE=ACGTATCCAGAAGGGACCAG
PRIMER_RIGHT_35_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_35=763,20
PRIMER_RIGHT_35=1517,20
PRIMER_LEFT_35_TM=59.014
PRIMER_RIGHT_35_TM=59.820
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=755
PRIMER_PAIR_36_PENALTY=1.179061
PRIMER_LEFT_36_PENALTY=0.000037
PRIMER_RIGHT_36_PENALTY=1.179024
PRIMER_LEFT_36_SEQUENCE=ACGCCGTATGGACTTTGTTC
PRIMER_RIGHT_36_SEQUENCE=TAAACCTGGTGATTGGGTGA
PRIMER_LEFT_36=823,20
PRIMER_RIGHT_36=1521,20
PRIMER_LEFT_36_TM=60.000
PRIMER_RIGHT_36_TM=58.821
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=699
PRIMER_PAIR_37_PENALTY=1.179061
PRIMER_LEFT_37_PENALTY=0.000037
PRIMER_RIGHT_37_PENALTY=1.179024
PRIMER_LEFT_37_SEQUENCE=GAACGCCGTATGGACTTTGT
PRIMER_RIGHT_37_SEQUENCE=TAAACCTGGTGATTGGGTGA
PRIMER_LEFT_37=821,20
PRIMER_RIGHT_37=1521,20
PRIMER_LEFT_37_TM=60.000
PRIMER_RIGHT_37_TM=58.821
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=701
PRIMER_PAIR_38_PENALTY=1.187561
PRIMER_LEFT_38_PENALTY=1.007886
PRIMER_RIGHT_38_PENALTY=0.179674
PRIMER_LEFT_38_SEQUENCE=GGACTTTGTTCCCGATGAGT
PRIMER_RIGHT_38_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_38=832,20
PRIMER_RIGHT_38=1517,20
PRIMER_LEFT_38_TM=58.992
PRIMER_RIGHT_38_TM=59.820
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=686
PRIMER_PAIR_39_PENALTY=1.195414
PRIMER_LEFT_39_PENALTY=0.985544
PRIMER_RIGHT_39_PENALTY=0.209870
PRIMER_LEFT_39_SEQUENCE=ACGTATCCAGAAGGGACCAG
PRIMER_RIGHT_39_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_39=763,20
PRIMER_RIGHT_39=1519,20
PRIMER_LEFT_39_TM=59.014
PRIMER_RIGHT_39_TM=60.210
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=757
PRIMER_PAIR_40_PENALTY=1.195414
PRIMER_LEFT_40_PENALTY=0.985544
PRIMER_RIGHT_40_PENALTY=0.209870
PRIMER_LEFT_40_SEQUENCE=ACGTATCCAGAAGGGACCAG
PRIMER_RIGHT_40_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_40=763,20
PRIMER_RIGHT_40=1520,20
PRIMER_LEFT_40_TM=59.014
PRIMER_RIGHT_40_TM=60.210
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=758
PRIMER_PAIR_41_PENALTY=1.200379
PRIMER_LEFT_41_PENALTY=1.020705
PRIMER_RIGHT_41_PENALTY=0.179674
PRIMER_LEFT_41_SEQUENCE=TGAAAGATGGGGCGTGTTAG
PRIMER_RIGHT_41_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_41=581,20
PRIMER_RIGHT_41=1517,20
PRIMER_LEFT_41_TM=61.021
PRIMER_RIGHT_41_TM=59.820
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=5.7000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=937
PRIMER_PAIR_42_PENALTY=1.212686
PRIMER_LEFT_42_PENALTY=0.000037
PRIMER_RIGHT_42_PENALTY=1.212650
PRIMER_LEFT_42_SEQUENCE=ACGCCGTATGGACTTTGTTC
PRIMER_RIGHT_42_SEQUENCE=TAAACCTGGTGATTGGGTGAA
PRIMER_LEFT_42=823,20
PRIMER_RIGHT_42=1521,21
PRIMER_LEFT_42_TM=60.000
PRIMER_RIGHT_42_TM=60.213
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=699
PRIMER_PAIR_43_PENALTY=1.212686
PRIMER_LEFT_43_PENALTY=0.000037
PRIMER_RIGHT_43_PENALTY=1.212650
PRIMER_LEFT_43_SEQUENCE=ACGCCGTATGGACTTTGTTC
PRIMER_RIGHT_43_SEQUENCE=TTAAACCTGGTGATTGGGTGA
PRIMER_LEFT_43=823,20
PRIMER_RIGHT_43=1522,21
PRIMER_LEFT_43_TM=60.000
PRIMER_RIGHT_43_TM=60.213
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=700
PRIMER_PAIR_44_PENALTY=1.212686
PRIMER_LEFT_44_PENALTY=0.000037
PRIMER_RIGHT_44_PENALTY=1.212650
PRIMER_LEFT_44_SEQUENCE=GAACGCCGTATGGACTTTGT
PRIMER_RIGHT_44_SEQUENCE=TAAACCTGGTGATTGGGTGAA
PRIMER_LEFT_44=821,20
PRIMER_RIGHT_44=1521,21
PRIMER_LEFT_44_TM=60.000
PRIMER_RIGHT_44_TM=60.213
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=701
PRIMER_PAIR_45_PENALTY=1.212686
PRIMER_LEFT_45_PENALTY=0.000037
PRIMER_RIGHT_45_PENALTY=1.212650
PRIMER_LEFT_45_SEQUENCE=GAACGCCGTATGGACTTTGT
PRIMER_RIGHT_45_SEQUENCE=TTAAACCTGGTGATTGGGTGA
PRIMER_LEFT_45=821,20
PRIMER_RIGHT_45=1522,21
PRIMER_LEFT_45_TM=60.000
PRIMER_RIGHT_45_TM=60.213
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=702
PRIMER_PAIR_46_PENALTY=1.214080
PRIMER_LEFT_46_PENALTY=1.034406
PRIMER_RIGHT_46_PENALTY=0.179674
PRIMER_LEFT_46_SEQUENCE=TGGACTTTGTTCCCGATGAG
PRIMER_RIGHT_46_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_46=831,20
PRIMER_RIGHT_46=1517,20
PRIMER_LEFT_46_TM=61.034
PRIMER_RIGHT_46_TM=59.820
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.6000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=687
PRIMER_PAIR_47_PENALTY=1.217756
PRIMER_LEFT_47_PENALTY=1.007886
PRIMER_RIGHT_47_PENALTY=0.209870
PRIMER_LEFT_47_SEQUENCE=GGACTTTGTTCCCGATGAGT
PRIMER_RIGHT_47_SEQUENCE=AACCTGGTGATTGGGTGAAA
PRIMER_LEFT_47=832,20
PRIMER_RIGHT_47=1519,20
PRIMER_LEFT_47_TM=58.992
PRIMER_RIGHT_47_TM=60.210
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=688
PRIMER_PAIR_48_PENALTY=1.217756
PRIMER_LEFT_48_PENALTY=1.007886
PRIMER_RIGHT_48_PENALTY=0.209870
PRIMER_LEFT_48_SEQUENCE=GGACTTTGTTCCCGATGAGT
PRIMER_RIGHT_48_SEQUENCE=AAACCTGGTGATTGGGTGAA
PRIMER_LEFT_48=832,20
PRIMER_RIGHT_48=1520,20
PRIMER_LEFT_48_TM=58.992
PRIMER_RIGHT_48_TM=60.210
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=689
PRIMER_PAIR_49_PENALTY=1.221419
PRIMER_LEFT_49_PENALTY=1.041745
PRIMER_RIGHT_49_PENALTY=0.179674
PRIMER_LEFT_49_SEQUENCE=TCTCCACCCACTTGATGAAAC
PRIMER_RIGHT_49_SEQUENCE=CCTGGTGATTGGGTGAAACT
PRIMER_LEFT_49=744,21
PRIMER_RIGHT_49=1517,20
PRIMER_LEFT_49_TM=59.958
PRIMER_RIGHT_49_TM=59.820
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=774
=
SEQUENCE_ID=MAGIC.329_4_4712746_4712884_wil-2_wu-0_20282_15647_TRUE
SEQUENCE_TARGET=950,238
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_15311823_15311832_kn-0_oy-0_12970_69078_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_12743446_12743894_mt-0_ct-1_13902_28552_TRUE
SEQUENCE_TARGET=950,548
SEQUENCE_TEMPLATE=ACAACCAAATCAAATTNATNTTCAATATCGCNACTCAGTTTTCNATGTACTTGGANTAAGAAACGAGGTAACTTCATTATATCNNNNNNNAGTNACGATTTCATAAANTCTTNCAAAANNANCAAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_15264163_15264389_po-0_bur-0_44505_10705_TRUE
SEQUENCE_TARGET=950,326
SEQUENCE_TEMPLATE=GAGTNTGNAATCANAAGACTAGCCAGAATGGATTATCNCAACAATCTTTGTCCTCAGCATCNTGCGAATGCATCNATCAACCATGATGTCCTTCCATTTTCTCCNGANACCAAGCTGTCAACTTTTTATCACGACTGCCAATCAACGGTAGATGTANATCCCAANGATTANATTAAACAGCTTAACTGTGAGGATTATNTNGGTGGGAAAAGTTACTTTGTCTCGTCCTCTTCACATTCTGGGGATAGAGCTACCTTAGCTGCATTNAGTGCATCGTGCGAAGGGGTCGANATTCCAGTCTCTCNGTCNGCCTTGAAGACAGTAGAGAAAAATCAGAGTCTGGAGNCTATGANGAAGGCTATTGATGAGGGTTTCNAACTTNGCTTCAACAATGANTGTTCCCAATGCGCAATATCAGGGGGCGCTTGTGGCTTTGATCAGAGNTTGAAAGCATTNGTTTGCTATTGTGCGGATGAGCCTCACGAGAGTACTTGCTATTCTGAAATAGTAAAGAAGAGTAATGATTCATCTTTATCTGCTTTACTGAATANTTTAGCTGACCTAAGTTAATCTGATCCTAAATTCNTCTCTGAATCTAATTGGCTAATGTCCTTCCATAAGAACCAGATCTCTTATCTTCCTCANAAACTCTCTTGACTTTCAAACTATGTTTTATGCNCCATTCTAANATTCTGTCTTAAATTTTAACTGATATTNTTCTCATGGTAGCTCATGCAGGTCTCTCCAAAAAAGGGAAAATAGGTATGTGTCCATCNTTTTTCCTTTCAAGGNAACTAATTTCAGTAGAACATACCTANNNNNNNNNNNNNNNNNNNNNNNNTCTTGTCTTTCCGTTTATCTCTAGGCATCGGATTCGNTTCTGGATTCTTGGGTGCCACNCTTATCGGCGGATGTTTACTNTGTATCTTCATCCGGAGAAGGAAGAAACTNGCANCACAATACACAAACAAAGGCCTATCAACAACAACACCTTACTCAANCAANTATACAATGTCAAACACTCCAACCTCCACAACAATCTCTGGCAGCAATCACTCTCTTGTACCTTCAATATCTAACCTTGGAAACGGNAGCGTCTACTCTGGAATTCAAGTCTTCAGNTACGAAGAACTCGAGGAAGCNACTGANAATTTCTCNAAAGAACTCGGAGATGGAGGCTTCGGTACNGTCTACTACGGTACACTAAAAGACGGACGNNCNGTAGCNGTNAAACGACTCTTCGAAAGATCANTAAAACGCGTNGAACAATTCAAGAACGAAATCGATATCTTGAAATCCNTAAAACANCCGAACCTAGTGATTCTATACGGATGCACAACAAGACATAGCAGAGAGTTACTCCTAGTNTACGAATACATNTCCAANGGCACACTCGCTGAACANCTCCATGGAAACCAAGCACAATCTCGCCCTATTTGTTGGCCTGCTCGACTCCAAATNGCCATCGNAACCGCNAGCGCCTTNTCCTATCTCCACGCCTCAGGTAACACATACTGAACANACACACATNACACATACACNCNATGAGATTAGNTTACTAATGATGAATTCTCTGATTCAGGGATCATACACAGNGACGTNAAGACTACNAANATTCTCTTAGATAGCAATTACCAAGTCAAAGTAGCTGATTTCGGACTCTCACGGTTGTTTCCGATGGATCAAACTCACATCTCCACTGCTCCACAAGGTACTCCAGGATATGTAGATCCTGAGTANTACCAATGTTACCGNNTNAACGAGAAGAGTGACGTNTACAGCTTCGGAGTCGTACTCTCNGAACTAATCTCATCNAAAGAAGCNGTNGATATCACCAGACACCGCCACGATATTAACCTNGCNAACATGGCGATTTCGAAAATCCAAAACGACGCCNTNCANGAGCTCGCGGATCTATCACTCGGATTCGCGAGAGATCCTTCGGTGAAGAAGATGATGTCGTCGGTTGCTGAATTAGCATTCCGATGTTTGCAACAGGAGAGAGACGTACGGCCATCGATGGATGAGATCGTTGAGGTTTTGAGAGNGATTCAGAAGGATGGGATAAGTGATTCGAAGGATGTGNTNGTNGAGATTGATGTGAACGGTGGTGATGATGTTGGATTGTTGAAGCATGGTGTTCCTCCGCCGTTATCGCCGGAGACTGATAAAACGACNGCGAGTAGTTCGAATACNACGGCGAGTTCGTTTTGAAGAGAGTAGGAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.276493
PRIMER_LEFT_0_PENALTY=0.256130
PRIMER_RIGHT_0_PENALTY=0.020364
PRIMER_LEFT_0_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_0_SEQUENCE=TGGAGCAGTGGAGATGTGAG
PRIMER_LEFT_0=928,20
PRIMER_RIGHT_0=1706,20
PRIMER_LEFT_0_TM=59.744
PRIMER_RIGHT_0_TM=59.980
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=779
PRIMER_PAIR_1_PENALTY=0.367207
PRIMER_LEFT_1_PENALTY=0.256130
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_1_SEQUENCE=ATCGGAAACAACCGTGAGAG
PRIMER_LEFT_1=928,20
PRIMER_RIGHT_1=1678,20
PRIMER_LEFT_1_TM=59.744
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=751
PRIMER_PAIR_2_PENALTY=0.402570
PRIMER_LEFT_2_PENALTY=0.167348
PRIMER_RIGHT_2_PENALTY=0.235222
PRIMER_LEFT_2_SEQUENCE=TTTGCTATTGTGCGGATGAG
PRIMER_RIGHT_2_SEQUENCE=ATAGGGCGAGATTGTGCTTG
PRIMER_LEFT_2=457,20
PRIMER_RIGHT_2=1435,20
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=60.235
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=979
PRIMER_PAIR_3_PENALTY=0.411008
PRIMER_LEFT_3_PENALTY=0.256130
PRIMER_RIGHT_3_PENALTY=0.154878
PRIMER_LEFT_3_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_3_SEQUENCE=AGGTTAATATCGTGGCGGTG
PRIMER_LEFT_3=928,20
PRIMER_RIGHT_3=1858,20
PRIMER_LEFT_3_TM=59.744
PRIMER_RIGHT_3_TM=59.845
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=931
PRIMER_PAIR_4_PENALTY=0.428899
PRIMER_LEFT_4_PENALTY=0.256130
PRIMER_RIGHT_4_PENALTY=0.172769
PRIMER_LEFT_4_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_4_SEQUENCE=GTGGAGCAGTGGAGATGTGA
PRIMER_LEFT_4=928,20
PRIMER_RIGHT_4=1707,20
PRIMER_LEFT_4_TM=59.744
PRIMER_RIGHT_4_TM=59.827
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=780
PRIMER_PAIR_5_PENALTY=0.491351
PRIMER_LEFT_5_PENALTY=0.256130
PRIMER_RIGHT_5_PENALTY=0.235222
PRIMER_LEFT_5_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_5_SEQUENCE=ATAGGGCGAGATTGTGCTTG
PRIMER_LEFT_5=928,20
PRIMER_RIGHT_5=1435,20
PRIMER_LEFT_5_TM=59.744
PRIMER_RIGHT_5_TM=60.235
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=508
PRIMER_PAIR_6_PENALTY=0.541175
PRIMER_LEFT_6_PENALTY=0.305953
PRIMER_RIGHT_6_PENALTY=0.235222
PRIMER_LEFT_6_SEQUENCE=GTTTGCTATTGTGCGGATGA
PRIMER_RIGHT_6_SEQUENCE=ATAGGGCGAGATTGTGCTTG
PRIMER_LEFT_6=456,20
PRIMER_RIGHT_6=1435,20
PRIMER_LEFT_6_TM=59.694
PRIMER_RIGHT_6_TM=60.235
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=980
PRIMER_PAIR_7_PENALTY=0.542790
PRIMER_LEFT_7_PENALTY=0.256130
PRIMER_RIGHT_7_PENALTY=0.286660
PRIMER_LEFT_7_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_7_SEQUENCE=GGTTAATATCGTGGCGGTGT
PRIMER_LEFT_7=928,20
PRIMER_RIGHT_7=1857,20
PRIMER_LEFT_7_TM=59.744
PRIMER_RIGHT_7_TM=59.713
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=930
PRIMER_PAIR_8_PENALTY=0.651574
PRIMER_LEFT_8_PENALTY=0.631211
PRIMER_RIGHT_8_PENALTY=0.020364
PRIMER_LEFT_8_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_8_SEQUENCE=TGGAGCAGTGGAGATGTGAG
PRIMER_LEFT_8=730,20
PRIMER_RIGHT_8=1706,20
PRIMER_LEFT_8_TM=59.369
PRIMER_RIGHT_8_TM=59.980
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=977
PRIMER_PAIR_9_PENALTY=0.689742
PRIMER_LEFT_9_PENALTY=0.256130
PRIMER_RIGHT_9_PENALTY=0.433612
PRIMER_LEFT_9_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_9_SEQUENCE=TAATATCGTGGCGGTGTCTG
PRIMER_LEFT_9=928,20
PRIMER_RIGHT_9=1854,20
PRIMER_LEFT_9_TM=59.744
PRIMER_RIGHT_9_TM=59.566
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=927
PRIMER_PAIR_10_PENALTY=0.701451
PRIMER_LEFT_10_PENALTY=0.256130
PRIMER_RIGHT_10_PENALTY=0.445321
PRIMER_LEFT_10_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_10_SEQUENCE=GGAAACAACCGTGAGAGTCC
PRIMER_LEFT_10=928,20
PRIMER_RIGHT_10=1675,20
PRIMER_LEFT_10_TM=59.744
PRIMER_RIGHT_10_TM=59.555
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=748
PRIMER_PAIR_11_PENALTY=0.742288
PRIMER_LEFT_11_PENALTY=0.631211
PRIMER_RIGHT_11_PENALTY=0.111077
PRIMER_LEFT_11_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_11_SEQUENCE=ATCGGAAACAACCGTGAGAG
PRIMER_LEFT_11=730,20
PRIMER_RIGHT_11=1678,20
PRIMER_LEFT_11_TM=59.369
PRIMER_RIGHT_11_TM=60.111
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=949
PRIMER_PAIR_12_PENALTY=0.803380
PRIMER_LEFT_12_PENALTY=0.167348
PRIMER_RIGHT_12_PENALTY=0.636033
PRIMER_LEFT_12_SEQUENCE=TTTGCTATTGTGCGGATGAG
PRIMER_RIGHT_12_SEQUENCE=CGAGATTGTGCTTGGTTTCC
PRIMER_LEFT_12=457,20
PRIMER_RIGHT_12=1429,20
PRIMER_LEFT_12_TM=59.833
PRIMER_RIGHT_12_TM=60.636
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=973
PRIMER_PAIR_13_PENALTY=0.803980
PRIMER_LEFT_13_PENALTY=0.631211
PRIMER_RIGHT_13_PENALTY=0.172769
PRIMER_LEFT_13_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_13_SEQUENCE=GTGGAGCAGTGGAGATGTGA
PRIMER_LEFT_13=730,20
PRIMER_RIGHT_13=1707,20
PRIMER_LEFT_13_TM=59.369
PRIMER_RIGHT_13_TM=59.827
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=978
PRIMER_PAIR_14_PENALTY=0.841519
PRIMER_LEFT_14_PENALTY=0.256130
PRIMER_RIGHT_14_PENALTY=0.585389
PRIMER_LEFT_14_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_14_SEQUENCE=CTTGTGGAGCAGTGGAGATG
PRIMER_LEFT_14=928,20
PRIMER_RIGHT_14=1710,20
PRIMER_LEFT_14_TM=59.744
PRIMER_RIGHT_14_TM=59.415
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=783
PRIMER_PAIR_15_PENALTY=0.866433
PRIMER_LEFT_15_PENALTY=0.631211
PRIMER_RIGHT_15_PENALTY=0.235222
PRIMER_LEFT_15_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_15_SEQUENCE=ATAGGGCGAGATTGTGCTTG
PRIMER_LEFT_15=730,20
PRIMER_RIGHT_15=1435,20
PRIMER_LEFT_15_TM=59.369
PRIMER_RIGHT_15_TM=60.235
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=706
PRIMER_PAIR_16_PENALTY=0.892162
PRIMER_LEFT_16_PENALTY=0.256130
PRIMER_RIGHT_16_PENALTY=0.636033
PRIMER_LEFT_16_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_16_SEQUENCE=CGAGATTGTGCTTGGTTTCC
PRIMER_LEFT_16=928,20
PRIMER_RIGHT_16=1429,20
PRIMER_LEFT_16_TM=59.744
PRIMER_RIGHT_16_TM=60.636
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=502
PRIMER_PAIR_17_PENALTY=0.941986
PRIMER_LEFT_17_PENALTY=0.305953
PRIMER_RIGHT_17_PENALTY=0.636033
PRIMER_LEFT_17_SEQUENCE=GTTTGCTATTGTGCGGATGA
PRIMER_RIGHT_17_SEQUENCE=CGAGATTGTGCTTGGTTTCC
PRIMER_LEFT_17=456,20
PRIMER_RIGHT_17=1429,20
PRIMER_LEFT_17_TM=59.694
PRIMER_RIGHT_17_TM=60.636
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=974
PRIMER_PAIR_18_PENALTY=0.945811
PRIMER_LEFT_18_PENALTY=0.256130
PRIMER_RIGHT_18_PENALTY=0.689681
PRIMER_LEFT_18_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_18_SEQUENCE=ACAACCGTGAGAGTCCGAAA
PRIMER_LEFT_18=928,20
PRIMER_RIGHT_18=1671,20
PRIMER_LEFT_18_TM=59.744
PRIMER_RIGHT_18_TM=60.690
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=9.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=744
PRIMER_PAIR_19_PENALTY=0.945811
PRIMER_LEFT_19_PENALTY=0.256130
PRIMER_RIGHT_19_PENALTY=0.689681
PRIMER_LEFT_19_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_19_SEQUENCE=TCGGAAACAACCGTGAGAGT
PRIMER_LEFT_19=928,20
PRIMER_RIGHT_19=1677,20
PRIMER_LEFT_19_TM=59.744
PRIMER_RIGHT_19_TM=60.690
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=750
PRIMER_PAIR_20_PENALTY=0.953271
PRIMER_LEFT_20_PENALTY=0.167348
PRIMER_RIGHT_20_PENALTY=0.785923
PRIMER_LEFT_20_SEQUENCE=TTTGCTATTGTGCGGATGAG
PRIMER_RIGHT_20_SEQUENCE=GCGAGATTGTGCTTGGTTTC
PRIMER_LEFT_20=457,20
PRIMER_RIGHT_20=1430,20
PRIMER_LEFT_20_TM=59.833
PRIMER_RIGHT_20_TM=60.786
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=974
PRIMER_PAIR_21_PENALTY=0.958289
PRIMER_LEFT_21_PENALTY=0.256130
PRIMER_RIGHT_21_PENALTY=0.702159
PRIMER_LEFT_21_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_21_SEQUENCE=TTGATCCATCGGAAACAACC
PRIMER_LEFT_21=928,20
PRIMER_RIGHT_21=1685,20
PRIMER_LEFT_21_TM=59.744
PRIMER_RIGHT_21_TM=60.702
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=758
PRIMER_PAIR_22_PENALTY=0.967314
PRIMER_LEFT_22_PENALTY=0.256130
PRIMER_RIGHT_22_PENALTY=0.711184
PRIMER_LEFT_22_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_22_SEQUENCE=GTGTTACCTGAGGCGTGGAG
PRIMER_LEFT_22=928,20
PRIMER_RIGHT_22=1510,20
PRIMER_LEFT_22_TM=59.744
PRIMER_RIGHT_22_TM=60.711
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=60.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=583
PRIMER_PAIR_23_PENALTY=1.047956
PRIMER_LEFT_23_PENALTY=1.027592
PRIMER_RIGHT_23_PENALTY=0.020364
PRIMER_LEFT_23_SEQUENCE=TGGTAGCTCATGCAGGTCTCT
PRIMER_RIGHT_23_SEQUENCE=TGGAGCAGTGGAGATGTGAG
PRIMER_LEFT_23=723,21
PRIMER_RIGHT_23=1706,20
PRIMER_LEFT_23_TM=60.028
PRIMER_RIGHT_23_TM=59.980
PRIMER_LEFT_23_GC_PERCENT=52.381
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=984
PRIMER_PAIR_24_PENALTY=1.063642
PRIMER_LEFT_24_PENALTY=1.043279
PRIMER_RIGHT_24_PENALTY=0.020364
PRIMER_LEFT_24_SEQUENCE=CTCATGCAGGTCTCTCCAAA
PRIMER_RIGHT_24_SEQUENCE=TGGAGCAGTGGAGATGTGAG
PRIMER_LEFT_24=729,20
PRIMER_RIGHT_24=1706,20
PRIMER_LEFT_24_TM=58.957
PRIMER_RIGHT_24_TM=59.980
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=978
PRIMER_PAIR_25_PENALTY=1.076532
PRIMER_LEFT_25_PENALTY=0.631211
PRIMER_RIGHT_25_PENALTY=0.445321
PRIMER_LEFT_25_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_25_SEQUENCE=GGAAACAACCGTGAGAGTCC
PRIMER_LEFT_25=730,20
PRIMER_RIGHT_25=1675,20
PRIMER_LEFT_25_TM=59.369
PRIMER_RIGHT_25_TM=59.555
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=946
PRIMER_PAIR_26_PENALTY=1.091876
PRIMER_LEFT_26_PENALTY=0.305953
PRIMER_RIGHT_26_PENALTY=0.785923
PRIMER_LEFT_26_SEQUENCE=GTTTGCTATTGTGCGGATGA
PRIMER_RIGHT_26_SEQUENCE=GCGAGATTGTGCTTGGTTTC
PRIMER_LEFT_26=456,20
PRIMER_RIGHT_26=1430,20
PRIMER_LEFT_26_TM=59.694
PRIMER_RIGHT_26_TM=60.786
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=975
PRIMER_PAIR_27_PENALTY=1.117521
PRIMER_LEFT_27_PENALTY=0.256130
PRIMER_RIGHT_27_PENALTY=0.861391
PRIMER_LEFT_27_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_27_SEQUENCE=ACCTTGTGGAGCAGTGGAGA
PRIMER_LEFT_27=928,20
PRIMER_RIGHT_27=1712,20
PRIMER_LEFT_27_TM=59.744
PRIMER_RIGHT_27_TM=60.861
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=785
PRIMER_PAIR_28_PENALTY=1.153521
PRIMER_LEFT_28_PENALTY=0.167348
PRIMER_RIGHT_28_PENALTY=0.986173
PRIMER_LEFT_28_SEQUENCE=TTTGCTATTGTGCGGATGAG
PRIMER_RIGHT_28_SEQUENCE=ATGTCTTGTTGTGCATCCGT
PRIMER_LEFT_28=457,20
PRIMER_RIGHT_28=1346,20
PRIMER_LEFT_28_TM=59.833
PRIMER_RIGHT_28_TM=59.014
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=890
PRIMER_PAIR_29_PENALTY=1.154356
PRIMER_LEFT_29_PENALTY=1.043279
PRIMER_RIGHT_29_PENALTY=0.111077
PRIMER_LEFT_29_SEQUENCE=CTCATGCAGGTCTCTCCAAA
PRIMER_RIGHT_29_SEQUENCE=ATCGGAAACAACCGTGAGAG
PRIMER_LEFT_29=729,20
PRIMER_RIGHT_29=1678,20
PRIMER_LEFT_29_TM=58.957
PRIMER_RIGHT_29_TM=60.111
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.8000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=950
PRIMER_PAIR_30_PENALTY=1.161140
PRIMER_LEFT_30_PENALTY=0.256130
PRIMER_RIGHT_30_PENALTY=0.905010
PRIMER_LEFT_30_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_30_SEQUENCE=TGTGGAGCAGTGGAGATGTG
PRIMER_LEFT_30=928,20
PRIMER_RIGHT_30=1708,20
PRIMER_LEFT_30_TM=59.744
PRIMER_RIGHT_30_TM=60.905
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=781
PRIMER_PAIR_31_PENALTY=1.172928
PRIMER_LEFT_31_PENALTY=1.152564
PRIMER_RIGHT_31_PENALTY=0.020364
PRIMER_LEFT_31_SEQUENCE=CATGCAGGTCTCTCCAAAAA
PRIMER_RIGHT_31_SEQUENCE=TGGAGCAGTGGAGATGTGAG
PRIMER_LEFT_31=731,20
PRIMER_RIGHT_31=1706,20
PRIMER_LEFT_31_TM=58.847
PRIMER_RIGHT_31_TM=59.980
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=976
PRIMER_PAIR_32_PENALTY=1.200361
PRIMER_LEFT_32_PENALTY=1.027592
PRIMER_RIGHT_32_PENALTY=0.172769
PRIMER_LEFT_32_SEQUENCE=TGGTAGCTCATGCAGGTCTCT
PRIMER_RIGHT_32_SEQUENCE=GTGGAGCAGTGGAGATGTGA
PRIMER_LEFT_32=723,21
PRIMER_RIGHT_32=1707,20
PRIMER_LEFT_32_TM=60.028
PRIMER_RIGHT_32_TM=59.827
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=985
PRIMER_PAIR_33_PENALTY=1.206832
PRIMER_LEFT_33_PENALTY=0.256130
PRIMER_RIGHT_33_PENALTY=0.950702
PRIMER_LEFT_33_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_33_SEQUENCE=TGAGTTTGATCCATCGGAAA
PRIMER_LEFT_33=928,20
PRIMER_RIGHT_33=1690,20
PRIMER_LEFT_33_TM=59.744
PRIMER_RIGHT_33_TM=59.049
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=763
PRIMER_PAIR_34_PENALTY=1.216048
PRIMER_LEFT_34_PENALTY=1.043279
PRIMER_RIGHT_34_PENALTY=0.172769
PRIMER_LEFT_34_SEQUENCE=CTCATGCAGGTCTCTCCAAA
PRIMER_RIGHT_34_SEQUENCE=GTGGAGCAGTGGAGATGTGA
PRIMER_LEFT_34=729,20
PRIMER_RIGHT_34=1707,20
PRIMER_LEFT_34_TM=58.957
PRIMER_RIGHT_34_TM=59.827
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=979
PRIMER_PAIR_35_PENALTY=1.231212
PRIMER_LEFT_35_PENALTY=0.256130
PRIMER_RIGHT_35_PENALTY=0.975083
PRIMER_LEFT_35_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_35_SEQUENCE=ATGTGTTACCTGAGGCGTGG
PRIMER_LEFT_35=928,20
PRIMER_RIGHT_35=1512,20
PRIMER_LEFT_35_TM=59.744
PRIMER_RIGHT_35_TM=60.975
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=585
PRIMER_PAIR_36_PENALTY=1.242303
PRIMER_LEFT_36_PENALTY=0.256130
PRIMER_RIGHT_36_PENALTY=0.986173
PRIMER_LEFT_36_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_36_SEQUENCE=ATGTCTTGTTGTGCATCCGT
PRIMER_LEFT_36=928,20
PRIMER_RIGHT_36=1346,20
PRIMER_LEFT_36_TM=59.744
PRIMER_RIGHT_36_TM=59.014
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=9.6000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=419
PRIMER_PAIR_37_PENALTY=1.262814
PRIMER_LEFT_37_PENALTY=1.027592
PRIMER_RIGHT_37_PENALTY=0.235222
PRIMER_LEFT_37_SEQUENCE=TGGTAGCTCATGCAGGTCTCT
PRIMER_RIGHT_37_SEQUENCE=ATAGGGCGAGATTGTGCTTG
PRIMER_LEFT_37=723,21
PRIMER_RIGHT_37=1435,20
PRIMER_LEFT_37_TM=60.028
PRIMER_RIGHT_37_TM=60.235
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=713
PRIMER_PAIR_38_PENALTY=1.263641
PRIMER_LEFT_38_PENALTY=1.152564
PRIMER_RIGHT_38_PENALTY=0.111077
PRIMER_LEFT_38_SEQUENCE=CATGCAGGTCTCTCCAAAAA
PRIMER_RIGHT_38_SEQUENCE=ATCGGAAACAACCGTGAGAG
PRIMER_LEFT_38=731,20
PRIMER_RIGHT_38=1678,20
PRIMER_LEFT_38_TM=58.847
PRIMER_RIGHT_38_TM=60.111
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=948
PRIMER_PAIR_39_PENALTY=1.267243
PRIMER_LEFT_39_PENALTY=0.631211
PRIMER_RIGHT_39_PENALTY=0.636033
PRIMER_LEFT_39_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_39_SEQUENCE=CGAGATTGTGCTTGGTTTCC
PRIMER_LEFT_39=730,20
PRIMER_RIGHT_39=1429,20
PRIMER_LEFT_39_TM=59.369
PRIMER_RIGHT_39_TM=60.636
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=700
PRIMER_PAIR_40_PENALTY=1.276390
PRIMER_LEFT_40_PENALTY=0.167348
PRIMER_RIGHT_40_PENALTY=1.109043
PRIMER_LEFT_40_SEQUENCE=TTTGCTATTGTGCGGATGAG
PRIMER_RIGHT_40_SEQUENCE=AAATAGGGCGAGATTGTGCTT
PRIMER_LEFT_40=457,20
PRIMER_RIGHT_40=1437,21
PRIMER_LEFT_40_TM=59.833
PRIMER_RIGHT_40_TM=60.109
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.6000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=981
PRIMER_PAIR_41_PENALTY=1.305275
PRIMER_LEFT_41_PENALTY=1.284911
PRIMER_RIGHT_41_PENALTY=0.020364
PRIMER_LEFT_41_SEQUENCE=CATCCGGAGAAGGAAGAAAC
PRIMER_RIGHT_41_SEQUENCE=TGGAGCAGTGGAGATGTGAG
PRIMER_LEFT_41=929,20
PRIMER_RIGHT_41=1706,20
PRIMER_LEFT_41_TM=58.715
PRIMER_RIGHT_41_TM=59.980
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=778
PRIMER_PAIR_42_PENALTY=1.320892
PRIMER_LEFT_42_PENALTY=0.631211
PRIMER_RIGHT_42_PENALTY=0.689681
PRIMER_LEFT_42_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_42_SEQUENCE=ACAACCGTGAGAGTCCGAAA
PRIMER_LEFT_42=730,20
PRIMER_RIGHT_42=1671,20
PRIMER_LEFT_42_TM=59.369
PRIMER_RIGHT_42_TM=60.690
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=9.0000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=942
PRIMER_PAIR_43_PENALTY=1.320892
PRIMER_LEFT_43_PENALTY=0.631211
PRIMER_RIGHT_43_PENALTY=0.689681
PRIMER_LEFT_43_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_43_SEQUENCE=TCGGAAACAACCGTGAGAGT
PRIMER_LEFT_43=730,20
PRIMER_RIGHT_43=1677,20
PRIMER_LEFT_43_TM=59.369
PRIMER_RIGHT_43_TM=60.690
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=6.1000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=948
PRIMER_PAIR_44_PENALTY=1.321464
PRIMER_LEFT_44_PENALTY=1.301100
PRIMER_RIGHT_44_PENALTY=0.020364
PRIMER_LEFT_44_SEQUENCE=CATCCGGAGAAGGAAGAAACT
PRIMER_RIGHT_44_SEQUENCE=TGGAGCAGTGGAGATGTGAG
PRIMER_LEFT_44=929,21
PRIMER_RIGHT_44=1706,20
PRIMER_LEFT_44_TM=59.699
PRIMER_RIGHT_44_TM=59.980
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=778
PRIMER_PAIR_45_PENALTY=1.325333
PRIMER_LEFT_45_PENALTY=1.152564
PRIMER_RIGHT_45_PENALTY=0.172769
PRIMER_LEFT_45_SEQUENCE=CATGCAGGTCTCTCCAAAAA
PRIMER_RIGHT_45_SEQUENCE=GTGGAGCAGTGGAGATGTGA
PRIMER_LEFT_45=731,20
PRIMER_RIGHT_45=1707,20
PRIMER_LEFT_45_TM=58.847
PRIMER_RIGHT_45_TM=59.827
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=977
PRIMER_PAIR_46_PENALTY=1.331523
PRIMER_LEFT_46_PENALTY=0.256130
PRIMER_RIGHT_46_PENALTY=1.075394
PRIMER_LEFT_46_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_46_SEQUENCE=TGAGTTTGATCCATCGGAAAC
PRIMER_LEFT_46=928,20
PRIMER_RIGHT_46=1690,21
PRIMER_LEFT_46_TM=59.744
PRIMER_RIGHT_46_TM=59.925
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=763
PRIMER_PAIR_47_PENALTY=1.331523
PRIMER_LEFT_47_PENALTY=0.256130
PRIMER_RIGHT_47_PENALTY=1.075394
PRIMER_LEFT_47_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_47_SEQUENCE=GTGAGTTTGATCCATCGGAAA
PRIMER_LEFT_47=928,20
PRIMER_RIGHT_47=1691,21
PRIMER_LEFT_47_TM=59.744
PRIMER_RIGHT_47_TM=59.925
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=764
PRIMER_PAIR_48_PENALTY=1.333370
PRIMER_LEFT_48_PENALTY=0.631211
PRIMER_RIGHT_48_PENALTY=0.702159
PRIMER_LEFT_48_SEQUENCE=TCATGCAGGTCTCTCCAAAA
PRIMER_RIGHT_48_SEQUENCE=TTGATCCATCGGAAACAACC
PRIMER_LEFT_48=730,20
PRIMER_RIGHT_48=1685,20
PRIMER_LEFT_48_TM=59.369
PRIMER_RIGHT_48_TM=60.702
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=956
PRIMER_PAIR_49_PENALTY=1.340798
PRIMER_LEFT_49_PENALTY=0.256130
PRIMER_RIGHT_49_PENALTY=1.084668
PRIMER_LEFT_49_SEQUENCE=TCATCCGGAGAAGGAAGAAA
PRIMER_RIGHT_49_SEQUENCE=CTGGAGTACCTTGTGGAGCAG
PRIMER_LEFT_49=928,20
PRIMER_RIGHT_49=1719,21
PRIMER_LEFT_49_TM=59.744
PRIMER_RIGHT_49_TM=59.915
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=57.143
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=792
=
SEQUENCE_ID=MAGIC.287_4_4260284_4260437_bur-0_mt-0_1475_33346_TRUE
SEQUENCE_TARGET=950,253
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14286771_14287043_hi-0_zu-0_14637_61782_TRUE
SEQUENCE_TARGET=950,372
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANGTTCATGTGNTTGCTGGTTAATTAAAAATATAGATGACAAANGCTAACGCTAATCTCTTTTAGAACACAAAAGTTGATGGTTCCTTGGTCANCAAATAANGNANATATATATATTTCTTTTATCTGCAATGTANACTGAACAAATGTTTTATCAATCTTAGACCAANNNAAAAAAAAAANNNATNTTTTATCAATCATGCCTTTGACACTATGTATAATATTGAAATAATACANAATATGTATGAGATGAAATATTTATGAAACTTAGCTTACTCAATATCTGAAAGACCCAGCCCAGNGTAAACAACAAANACTTTTAAACTTNANCNTTGCTGAAGANCTCTNGTTTGTGAATTNGATTGTCAAAATTCTTNTAGANAACCTTCAAAAAANCTAACANAGTACTAATTAGATAGNAGAATAAAAGCAAAGANCTAATGTAAATTGTTTCAAACAAAAGNAGAAACTTACAAAGNCGAGTATATGGTTGGAGGAGATAGAACCTACNATNGAANTGAATTTAGCCATATAGATAGATATGAACTAATATTTTATGAAGAACTACATATATTTTNTAAAGACAAANAAGAGAAAGATAATAATAAACTANATTGAATGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGAGAAAGTTATTTTAAATAGGACCTATTGNCAAATGCTTTTATATTGGAAATGAGATTGGTTAATGGAAAAAGAAAACCGTGGAGAAAGTGGTTAGTTTCNTTGATCGAAATGATCGTGGAANGATGGGGAAAACTGGAAAATGATAGTTGGAAGAAACTAAAAAGAATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4767805_4767947_oy-0_mt-0_37657_546318_TRUE
SEQUENCE_TARGET=950,242
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_15381963_15382090_can-0_wu-0_38401_6647495_TRUE
SEQUENCE_TARGET=950,227
SEQUENCE_TEMPLATE=AATTTAAAGGAGCCACAATGTTGTTTAAAGGAGCTAAACGAAAAATAAGATTTCAAAGANNTAAGGGCAAAAATGAAATGAAATGATAACTATAATTATAGAGAAACTGATAGGAATAACATTTTCACTTTCAAACAATAGTTCCTCNACTNTTAAGTCGGCAAATAATTTCTTAAATTTGCATTGACCACATAGAAATGGGTTCCAGAATAATCAACGCTGAATATTCCAGAAAGGGGATTAAGCTTCGGTGAAGCTTGGGGGCTTGTAGGCAATGAAACTGATGCATTGGACTTGACGGGTGTTGTCGAATCCGATGATCCTAATGAAGGCGCCAGGGTACTCCTTCTTGCATTCTTCAACTTCCTTCAACACTTGNGCGGAGTCGGTGCATCCGAACAATGGAAGCTTCCACATTGTCCAGTANCGTCCATCATAGTATCCGGGAGTGTTTCCGTGCTCACGGTACACAAATCCGTGCTATATATAAAGAAAACCATTCAATACAANTGATATATATATTGGTGANAGTCTATTTCGGTTATCCACTTAAGTTTCTAGCAATGAAGAANAAGAATAAAGGATGCTTAGAGACAAAGGAAAAGTTCAGTGTATATTACCTCCAACTCGAATTCAACACAAGGAATCCACTTGTTGCGGAGAAGGTAGTCAACTTCCTTAGCCAATTCAACGTCACTAAGGTCAGGGAGGTAAGATAGAGTCTCAAACTTCTTCTTTCCGATTGGTGGCCACACCTATAAATTCGAATTCCCAATTATTCTAAAGTATTGTTCAGTTGCACTAATTGCACATNACAAAAGACAGTTTTCATTAATGACNTTTACCTTCATGCAGCTAACTCTTCCTCCGTTGNTTGTGATGGAAGTAATGTCGTTGTTGGCCTTGCGGGTNACCGGGAAAGAAGCGGATGACTTCAAGCCGGTGAATGGAGCGACCATGGTGGCTTGAGCCGGGGAGGTAACCACAGCGGTGGAGGAGANCATAGAGGAAGCCATTACTTCTTCTTNTTGTTTCTCTTCTTCTTTTGGTTTTTCTCTTACTTACTTGTTTGTCTNTTGNTTTNGTCATCTTTATATAGTGNGAACCGCTAGAGGCAANTAGCCCTANTGATCAAAGGGGCCATAATTCATGATAACGTCGTTAATTGCCACAGGATTAAGGAAAGGTGTTGACACCCTTATCGATTTGGACCACTAGGATAAGNTCACGTGGCAGAAAACTCCATAATCTTATCTTTCTCNTGTAAGGGTANGAGTGAATNTGTATGGNTACTGNTCTCCACTTGATCGGTTGCTTTTGGCTCCTTTCNNNTGGGAGGCGTCAAATTGTAGATATCGGTTAAANACTTTTTACNTNNNNNNNNAAAAAATGATTAAATTTCTATTACCNNNNNTTAACACANTCATANTTTTATTTNTCCACTTGATAATCTTAAATAATAAGAATATAAGTANANTNNTTNAATTCNTCCCANATTTTAAAAGTATANTTANATCAGACAGANTTCATTCANNNTGGTTTTTAATTGGGANATGTAAACTAGTATTTTGTNTTATTATTNTCTANGTAGTNTGNACGATCACTAGAACTTTTANTCACATAATGGATATTAAATTANAAAACAATCCAAATGCTTGTGAATTTTCAGGTTTTACGGCTAATTAATANATTTCTGATGAACAAGAGGAACCCNCGGCACCNTTNCTTTTGGAGCACNANGCNATTGCACTTACGGCCCACTTAAGCACGAATGTGGCNCCTCAAGNTAATTNCCACCATGNCAACTTAAGCACAAAAGNCATGACGGATCNNGGGGAAATTTTATANCACACTTCAATNGATGGTGTGGATCCGAGGNAAGAATCNNCANTCTAAGNCCACATGAAGAAGCTCNTATGGTCATGGNTCCACGAATACCANNTAANAGCTCATATATGCCCCGNTNCAACCNTATNAGAAGAAGTAGCCAANCNCNTNCANGAAACATTGATNNCTTTTCATTNTAAATAANTGTTTTAGCTTTGAATGTATNGTANNNNNNNNNNNNNNNNNNAAAACAGNTNCCAAATTATAAATATTCNTGCTTTGTTATCTNCATTANAAATT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.117736
PRIMER_LEFT_0_PENALTY=0.044122
PRIMER_RIGHT_0_PENALTY=0.073614
PRIMER_LEFT_0_SEQUENCE=TTCTTCTTTCCGATTGGTGG
PRIMER_RIGHT_0_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_0=729,20
PRIMER_RIGHT_0=1352,20
PRIMER_LEFT_0_TM=60.044
PRIMER_RIGHT_0_TM=60.074
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=624
PRIMER_PAIR_1_PENALTY=0.298736
PRIMER_LEFT_1_PENALTY=0.044122
PRIMER_RIGHT_1_PENALTY=0.254613
PRIMER_LEFT_1_SEQUENCE=TTCTTCTTTCCGATTGGTGG
PRIMER_RIGHT_1_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_1=729,20
PRIMER_RIGHT_1=1316,20
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=60.255
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=588
PRIMER_PAIR_2_PENALTY=0.488770
PRIMER_LEFT_2_PENALTY=0.415156
PRIMER_RIGHT_2_PENALTY=0.073614
PRIMER_LEFT_2_SEQUENCE=TAATGTCGTTGTTGGCCTTG
PRIMER_RIGHT_2_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_2=886,20
PRIMER_RIGHT_2=1352,20
PRIMER_LEFT_2_TM=59.585
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=467
PRIMER_PAIR_3_PENALTY=0.502095
PRIMER_LEFT_3_PENALTY=0.428481
PRIMER_RIGHT_3_PENALTY=0.073614
PRIMER_LEFT_3_SEQUENCE=TGCTCACGGTACACAAATCC
PRIMER_RIGHT_3_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_3=457,20
PRIMER_RIGHT_3=1352,20
PRIMER_LEFT_3_TM=59.572
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=896
PRIMER_PAIR_4_PENALTY=0.633599
PRIMER_LEFT_4_PENALTY=0.559985
PRIMER_RIGHT_4_PENALTY=0.073614
PRIMER_LEFT_4_SEQUENCE=TGTTGCGGAGAAGGTAGTCA
PRIMER_RIGHT_4_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_4=652,20
PRIMER_RIGHT_4=1352,20
PRIMER_LEFT_4_TM=59.440
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=701
PRIMER_PAIR_5_PENALTY=0.646480
PRIMER_LEFT_5_PENALTY=0.415156
PRIMER_RIGHT_5_PENALTY=0.231323
PRIMER_LEFT_5_SEQUENCE=TAATGTCGTTGTTGGCCTTG
PRIMER_RIGHT_5_SEQUENCE=GCTTAAGTGGGCCGTAAGTG
PRIMER_LEFT_5=886,20
PRIMER_RIGHT_5=1766,20
PRIMER_LEFT_5_TM=59.585
PRIMER_RIGHT_5_TM=59.769
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=881
PRIMER_PAIR_6_PENALTY=0.651982
PRIMER_LEFT_6_PENALTY=0.578368
PRIMER_RIGHT_6_PENALTY=0.073614
PRIMER_LEFT_6_SEQUENCE=GGGAAAGAAGCGGATGACTT
PRIMER_RIGHT_6_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_6=915,20
PRIMER_RIGHT_6=1352,20
PRIMER_LEFT_6_TM=60.578
PRIMER_RIGHT_6_TM=60.074
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=438
PRIMER_PAIR_7_PENALTY=0.669770
PRIMER_LEFT_7_PENALTY=0.415156
PRIMER_RIGHT_7_PENALTY=0.254613
PRIMER_LEFT_7_SEQUENCE=TAATGTCGTTGTTGGCCTTG
PRIMER_RIGHT_7_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_7=886,20
PRIMER_RIGHT_7=1316,20
PRIMER_LEFT_7_TM=59.585
PRIMER_RIGHT_7_TM=60.255
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=431
PRIMER_PAIR_8_PENALTY=0.683094
PRIMER_LEFT_8_PENALTY=0.428481
PRIMER_RIGHT_8_PENALTY=0.254613
PRIMER_LEFT_8_SEQUENCE=TGCTCACGGTACACAAATCC
PRIMER_RIGHT_8_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_8=457,20
PRIMER_RIGHT_8=1316,20
PRIMER_LEFT_8_TM=59.572
PRIMER_RIGHT_8_TM=60.255
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=860
PRIMER_PAIR_9_PENALTY=0.709647
PRIMER_LEFT_9_PENALTY=0.636033
PRIMER_RIGHT_9_PENALTY=0.073614
PRIMER_LEFT_9_SEQUENCE=AATCCACTTGTTGCGGAGAA
PRIMER_RIGHT_9_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_9=644,20
PRIMER_RIGHT_9=1352,20
PRIMER_LEFT_9_TM=60.636
PRIMER_RIGHT_9_TM=60.074
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=709
PRIMER_PAIR_10_PENALTY=0.789446
PRIMER_LEFT_10_PENALTY=0.033905
PRIMER_RIGHT_10_PENALTY=0.755541
PRIMER_LEFT_10_SEQUENCE=GAAAGGGGATTAAGCTTCGG
PRIMER_RIGHT_10_SEQUENCE=GGTCCAAATCGATAAGGGTG
PRIMER_LEFT_10=231,20
PRIMER_RIGHT_10=1212,20
PRIMER_LEFT_10_TM=60.034
PRIMER_RIGHT_10_TM=59.244
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=10.2000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=982
PRIMER_PAIR_11_PENALTY=0.799664
PRIMER_LEFT_11_PENALTY=0.044122
PRIMER_RIGHT_11_PENALTY=0.755541
PRIMER_LEFT_11_SEQUENCE=TTCTTCTTTCCGATTGGTGG
PRIMER_RIGHT_11_SEQUENCE=GGTCCAAATCGATAAGGGTG
PRIMER_LEFT_11=729,20
PRIMER_RIGHT_11=1212,20
PRIMER_LEFT_11_TM=60.044
PRIMER_RIGHT_11_TM=59.244
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=484
PRIMER_PAIR_12_PENALTY=0.809691
PRIMER_LEFT_12_PENALTY=0.578368
PRIMER_RIGHT_12_PENALTY=0.231323
PRIMER_LEFT_12_SEQUENCE=GGGAAAGAAGCGGATGACTT
PRIMER_RIGHT_12_SEQUENCE=GCTTAAGTGGGCCGTAAGTG
PRIMER_LEFT_12=915,20
PRIMER_RIGHT_12=1766,20
PRIMER_LEFT_12_TM=60.578
PRIMER_RIGHT_12_TM=59.769
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=852
PRIMER_PAIR_13_PENALTY=0.814598
PRIMER_LEFT_13_PENALTY=0.559985
PRIMER_RIGHT_13_PENALTY=0.254613
PRIMER_LEFT_13_SEQUENCE=TGTTGCGGAGAAGGTAGTCA
PRIMER_RIGHT_13_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_13=652,20
PRIMER_RIGHT_13=1316,20
PRIMER_LEFT_13_TM=59.440
PRIMER_RIGHT_13_TM=60.255
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=665
PRIMER_PAIR_14_PENALTY=0.832981
PRIMER_LEFT_14_PENALTY=0.578368
PRIMER_RIGHT_14_PENALTY=0.254613
PRIMER_LEFT_14_SEQUENCE=GGGAAAGAAGCGGATGACTT
PRIMER_RIGHT_14_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_14=915,20
PRIMER_RIGHT_14=1316,20
PRIMER_LEFT_14_TM=60.578
PRIMER_RIGHT_14_TM=60.255
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=402
PRIMER_PAIR_15_PENALTY=1.101258
PRIMER_LEFT_15_PENALTY=0.033905
PRIMER_RIGHT_15_PENALTY=1.067353
PRIMER_LEFT_15_SEQUENCE=GAAAGGGGATTAAGCTTCGG
PRIMER_RIGHT_15_SEQUENCE=GGTCCAAATCGATAAGGGTGT
PRIMER_LEFT_15=231,20
PRIMER_RIGHT_15=1212,21
PRIMER_LEFT_15_TM=60.034
PRIMER_RIGHT_15_TM=60.067
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.2000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=982
PRIMER_PAIR_16_PENALTY=1.106358
PRIMER_LEFT_16_PENALTY=1.032744
PRIMER_RIGHT_16_PENALTY=0.073614
PRIMER_LEFT_16_SEQUENCE=GCAGCTAACTCTTCCTCCGTT
PRIMER_RIGHT_16_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_16=851,21
PRIMER_RIGHT_16=1352,20
PRIMER_LEFT_16_TM=60.033
PRIMER_RIGHT_16_TM=60.074
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=502
PRIMER_PAIR_17_PENALTY=1.111475
PRIMER_LEFT_17_PENALTY=0.044122
PRIMER_RIGHT_17_PENALTY=1.067353
PRIMER_LEFT_17_SEQUENCE=TTCTTCTTTCCGATTGGTGG
PRIMER_RIGHT_17_SEQUENCE=GGTCCAAATCGATAAGGGTGT
PRIMER_LEFT_17=729,20
PRIMER_RIGHT_17=1212,21
PRIMER_LEFT_17_TM=60.044
PRIMER_RIGHT_17_TM=60.067
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=484
PRIMER_PAIR_18_PENALTY=1.142799
PRIMER_LEFT_18_PENALTY=0.044122
PRIMER_RIGHT_18_PENALTY=1.098677
PRIMER_LEFT_18_SEQUENCE=TTCTTCTTTCCGATTGGTGG
PRIMER_RIGHT_18_SEQUENCE=GAAAGGAGCCAAAAGCAACC
PRIMER_LEFT_18=729,20
PRIMER_RIGHT_18=1328,20
PRIMER_LEFT_18_TM=60.044
PRIMER_RIGHT_18_TM=61.099
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=600
PRIMER_PAIR_19_PENALTY=1.152133
PRIMER_LEFT_19_PENALTY=1.078519
PRIMER_RIGHT_19_PENALTY=0.073614
PRIMER_LEFT_19_SEQUENCE=CACTTGTTGCGGAGAAGGTAG
PRIMER_RIGHT_19_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_19=648,21
PRIMER_RIGHT_19=1352,20
PRIMER_LEFT_19_TM=59.921
PRIMER_RIGHT_19_TM=60.074
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=705
PRIMER_PAIR_20_PENALTY=1.181350
PRIMER_LEFT_20_PENALTY=0.425809
PRIMER_RIGHT_20_PENALTY=0.755541
PRIMER_LEFT_20_SEQUENCE=GGGCTTGTAGGCAATGAAAC
PRIMER_RIGHT_20_SEQUENCE=GGTCCAAATCGATAAGGGTG
PRIMER_LEFT_20=261,20
PRIMER_RIGHT_20=1212,20
PRIMER_LEFT_20_TM=59.574
PRIMER_RIGHT_20_TM=59.244
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=952
PRIMER_PAIR_21_PENALTY=1.184022
PRIMER_LEFT_21_PENALTY=0.428481
PRIMER_RIGHT_21_PENALTY=0.755541
PRIMER_LEFT_21_SEQUENCE=TGCTCACGGTACACAAATCC
PRIMER_RIGHT_21_SEQUENCE=GGTCCAAATCGATAAGGGTG
PRIMER_LEFT_21=457,20
PRIMER_RIGHT_21=1212,20
PRIMER_LEFT_21_TM=59.572
PRIMER_RIGHT_21_TM=59.244
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=756
PRIMER_PAIR_22_PENALTY=1.198489
PRIMER_LEFT_22_PENALTY=0.442948
PRIMER_RIGHT_22_PENALTY=0.755541
PRIMER_LEFT_22_SEQUENCE=AAGGGGATTAAGCTTCGGTG
PRIMER_RIGHT_22_SEQUENCE=GGTCCAAATCGATAAGGGTG
PRIMER_LEFT_22=233,20
PRIMER_RIGHT_22=1212,20
PRIMER_LEFT_22_TM=60.443
PRIMER_RIGHT_22_TM=59.244
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.9000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=980
PRIMER_PAIR_23_PENALTY=1.207674
PRIMER_LEFT_23_PENALTY=1.134060
PRIMER_RIGHT_23_PENALTY=0.073614
PRIMER_LEFT_23_SEQUENCE=GTATCCGGGAGTGTTTCCGT
PRIMER_RIGHT_23_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_23=438,20
PRIMER_RIGHT_23=1352,20
PRIMER_LEFT_23_TM=61.134
PRIMER_RIGHT_23_TM=60.074
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.6000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=915
PRIMER_PAIR_24_PENALTY=1.216348
PRIMER_LEFT_24_PENALTY=0.415156
PRIMER_RIGHT_24_PENALTY=0.801192
PRIMER_LEFT_24_SEQUENCE=TAATGTCGTTGTTGGCCTTG
PRIMER_RIGHT_24_SEQUENCE=AGCAACCGATCAAGTGGAGA
PRIMER_LEFT_24=886,20
PRIMER_RIGHT_24=1315,20
PRIMER_LEFT_24_TM=59.585
PRIMER_RIGHT_24_TM=60.801
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=430
PRIMER_PAIR_25_PENALTY=1.225076
PRIMER_LEFT_25_PENALTY=0.033905
PRIMER_RIGHT_25_PENALTY=1.191170
PRIMER_LEFT_25_SEQUENCE=GAAAGGGGATTAAGCTTCGG
PRIMER_RIGHT_25_SEQUENCE=CCAAATCGATAAGGGTGTCAA
PRIMER_LEFT_25=231,20
PRIMER_RIGHT_25=1209,21
PRIMER_LEFT_25_TM=60.034
PRIMER_RIGHT_25_TM=59.809
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=10.2000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=979
PRIMER_PAIR_26_PENALTY=1.229673
PRIMER_LEFT_26_PENALTY=0.428481
PRIMER_RIGHT_26_PENALTY=0.801192
PRIMER_LEFT_26_SEQUENCE=TGCTCACGGTACACAAATCC
PRIMER_RIGHT_26_SEQUENCE=AGCAACCGATCAAGTGGAGA
PRIMER_LEFT_26=457,20
PRIMER_RIGHT_26=1315,20
PRIMER_LEFT_26_TM=59.572
PRIMER_RIGHT_26_TM=60.801
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.1000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=859
PRIMER_PAIR_27_PENALTY=1.235293
PRIMER_LEFT_27_PENALTY=0.044122
PRIMER_RIGHT_27_PENALTY=1.191170
PRIMER_LEFT_27_SEQUENCE=TTCTTCTTTCCGATTGGTGG
PRIMER_RIGHT_27_SEQUENCE=CCAAATCGATAAGGGTGTCAA
PRIMER_LEFT_27=729,20
PRIMER_RIGHT_27=1209,21
PRIMER_LEFT_27_TM=60.044
PRIMER_RIGHT_27_TM=59.809
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=481
PRIMER_PAIR_28_PENALTY=1.261603
PRIMER_LEFT_28_PENALTY=1.187989
PRIMER_RIGHT_28_PENALTY=0.073614
PRIMER_LEFT_28_SEQUENCE=GAATCCACTTGTTGCGGAGA
PRIMER_RIGHT_28_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_28=643,20
PRIMER_RIGHT_28=1352,20
PRIMER_LEFT_28_TM=61.188
PRIMER_RIGHT_28_TM=60.074
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=710
PRIMER_PAIR_29_PENALTY=1.264068
PRIMER_LEFT_29_PENALTY=1.032744
PRIMER_RIGHT_29_PENALTY=0.231323
PRIMER_LEFT_29_SEQUENCE=GCAGCTAACTCTTCCTCCGTT
PRIMER_RIGHT_29_SEQUENCE=GCTTAAGTGGGCCGTAAGTG
PRIMER_LEFT_29=851,21
PRIMER_RIGHT_29=1766,20
PRIMER_LEFT_29_TM=60.033
PRIMER_RIGHT_29_TM=59.769
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.9000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=916
PRIMER_PAIR_30_PENALTY=1.302787
PRIMER_LEFT_30_PENALTY=1.229173
PRIMER_RIGHT_30_PENALTY=0.073614
PRIMER_LEFT_30_SEQUENCE=CCTTAGCCAATTCAACGTCA
PRIMER_RIGHT_30_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_30=676,20
PRIMER_RIGHT_30=1352,20
PRIMER_LEFT_30_TM=58.771
PRIMER_RIGHT_30_TM=60.074
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.4000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=677
PRIMER_PAIR_31_PENALTY=1.315526
PRIMER_LEFT_31_PENALTY=0.559985
PRIMER_RIGHT_31_PENALTY=0.755541
PRIMER_LEFT_31_SEQUENCE=TGTTGCGGAGAAGGTAGTCA
PRIMER_RIGHT_31_SEQUENCE=GGTCCAAATCGATAAGGGTG
PRIMER_LEFT_31=652,20
PRIMER_RIGHT_31=1212,20
PRIMER_LEFT_31_TM=59.440
PRIMER_RIGHT_31_TM=59.244
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=561
PRIMER_PAIR_32_PENALTY=1.333132
PRIMER_LEFT_32_PENALTY=1.078519
PRIMER_RIGHT_32_PENALTY=0.254613
PRIMER_LEFT_32_SEQUENCE=CACTTGTTGCGGAGAAGGTAG
PRIMER_RIGHT_32_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_32=648,21
PRIMER_RIGHT_32=1316,20
PRIMER_LEFT_32_TM=59.921
PRIMER_RIGHT_32_TM=60.255
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=669
PRIMER_PAIR_33_PENALTY=1.361176
PRIMER_LEFT_33_PENALTY=0.559985
PRIMER_RIGHT_33_PENALTY=0.801192
PRIMER_LEFT_33_SEQUENCE=TGTTGCGGAGAAGGTAGTCA
PRIMER_RIGHT_33_SEQUENCE=AGCAACCGATCAAGTGGAGA
PRIMER_LEFT_33=652,20
PRIMER_RIGHT_33=1315,20
PRIMER_LEFT_33_TM=59.440
PRIMER_RIGHT_33_TM=60.801
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=664
PRIMER_PAIR_34_PENALTY=1.376346
PRIMER_LEFT_34_PENALTY=1.302732
PRIMER_RIGHT_34_PENALTY=0.073614
PRIMER_LEFT_34_SEQUENCE=GCAGCTAACTCTTCCTCCGT
PRIMER_RIGHT_34_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_34=851,20
PRIMER_RIGHT_34=1352,20
PRIMER_LEFT_34_TM=58.697
PRIMER_RIGHT_34_TM=60.074
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.6000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=502
PRIMER_PAIR_35_PENALTY=1.377879
PRIMER_LEFT_35_PENALTY=0.044122
PRIMER_RIGHT_35_PENALTY=1.333757
PRIMER_LEFT_35_SEQUENCE=TTCTTCTTTCCGATTGGTGG
PRIMER_RIGHT_35_SEQUENCE=ATCTACAATTTGACGCCTCCC
PRIMER_LEFT_35=729,20
PRIMER_RIGHT_35=1353,21
PRIMER_LEFT_35_TM=60.044
PRIMER_RIGHT_35_TM=60.334
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=625
PRIMER_PAIR_36_PENALTY=1.379560
PRIMER_LEFT_36_PENALTY=0.578368
PRIMER_RIGHT_36_PENALTY=0.801192
PRIMER_LEFT_36_SEQUENCE=GGGAAAGAAGCGGATGACTT
PRIMER_RIGHT_36_SEQUENCE=AGCAACCGATCAAGTGGAGA
PRIMER_LEFT_36=915,20
PRIMER_RIGHT_36=1315,20
PRIMER_LEFT_36_TM=60.578
PRIMER_RIGHT_36_TM=60.801
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=401
PRIMER_PAIR_37_PENALTY=1.388673
PRIMER_LEFT_37_PENALTY=1.134060
PRIMER_RIGHT_37_PENALTY=0.254613
PRIMER_LEFT_37_SEQUENCE=GTATCCGGGAGTGTTTCCGT
PRIMER_RIGHT_37_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_37=438,20
PRIMER_RIGHT_37=1316,20
PRIMER_LEFT_37_TM=61.134
PRIMER_RIGHT_37_TM=60.255
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=879
PRIMER_PAIR_38_PENALTY=1.391574
PRIMER_LEFT_38_PENALTY=0.636033
PRIMER_RIGHT_38_PENALTY=0.755541
PRIMER_LEFT_38_SEQUENCE=AATCCACTTGTTGCGGAGAA
PRIMER_RIGHT_38_SEQUENCE=GGTCCAAATCGATAAGGGTG
PRIMER_LEFT_38=644,20
PRIMER_RIGHT_38=1212,20
PRIMER_LEFT_38_TM=60.636
PRIMER_RIGHT_38_TM=59.244
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=569
PRIMER_PAIR_39_PENALTY=1.450390
PRIMER_LEFT_39_PENALTY=1.376776
PRIMER_RIGHT_39_PENALTY=0.073614
PRIMER_LEFT_39_SEQUENCE=GCCAATTCAACGTCACTAAGG
PRIMER_RIGHT_39_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_39=681,21
PRIMER_RIGHT_39=1352,20
PRIMER_LEFT_39_TM=59.623
PRIMER_RIGHT_39_TM=60.074
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.5000
PRIMER_RIGHT_39_END_STABILITY=9.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=672
PRIMER_PAIR_40_PENALTY=1.483786
PRIMER_LEFT_40_PENALTY=1.229173
PRIMER_RIGHT_40_PENALTY=0.254613
PRIMER_LEFT_40_SEQUENCE=CCTTAGCCAATTCAACGTCA
PRIMER_RIGHT_40_SEQUENCE=AAGCAACCGATCAAGTGGAG
PRIMER_LEFT_40=676,20
PRIMER_RIGHT_40=1316,20
PRIMER_LEFT_40_TM=58.771
PRIMER_RIGHT_40_TM=60.255
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.4000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=641
PRIMER_PAIR_41_PENALTY=1.487145
PRIMER_LEFT_41_PENALTY=1.413531
PRIMER_RIGHT_41_PENALTY=0.073614
PRIMER_LEFT_41_SEQUENCE=GTAATGTCGTTGTTGGCCTTG
PRIMER_RIGHT_41_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_41=885,21
PRIMER_RIGHT_41=1352,20
PRIMER_LEFT_41_TM=60.414
PRIMER_RIGHT_41_TM=60.074
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=468
PRIMER_PAIR_42_PENALTY=1.490598
PRIMER_LEFT_42_PENALTY=1.416984
PRIMER_RIGHT_42_PENALTY=0.073614
PRIMER_LEFT_42_SEQUENCE=CCTCCAACTCGAATTCAACAC
PRIMER_RIGHT_42_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_42=619,21
PRIMER_RIGHT_42=1352,20
PRIMER_LEFT_42_TM=59.583
PRIMER_RIGHT_42_TM=60.074
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=734
PRIMER_PAIR_43_PENALTY=1.493162
PRIMER_LEFT_43_PENALTY=0.425809
PRIMER_RIGHT_43_PENALTY=1.067353
PRIMER_LEFT_43_SEQUENCE=GGGCTTGTAGGCAATGAAAC
PRIMER_RIGHT_43_SEQUENCE=GGTCCAAATCGATAAGGGTGT
PRIMER_LEFT_43=261,20
PRIMER_RIGHT_43=1212,21
PRIMER_LEFT_43_TM=59.574
PRIMER_RIGHT_43_TM=60.067
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=952
PRIMER_PAIR_44_PENALTY=1.495834
PRIMER_LEFT_44_PENALTY=0.428481
PRIMER_RIGHT_44_PENALTY=1.067353
PRIMER_LEFT_44_SEQUENCE=TGCTCACGGTACACAAATCC
PRIMER_RIGHT_44_SEQUENCE=GGTCCAAATCGATAAGGGTGT
PRIMER_LEFT_44=457,20
PRIMER_RIGHT_44=1212,21
PRIMER_LEFT_44_TM=59.572
PRIMER_RIGHT_44_TM=60.067
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=756
PRIMER_PAIR_45_PENALTY=1.507554
PRIMER_LEFT_45_PENALTY=1.433940
PRIMER_RIGHT_45_PENALTY=0.073614
PRIMER_LEFT_45_SEQUENCE=GTGCTCACGGTACACAAATCC
PRIMER_RIGHT_45_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_45=456,21
PRIMER_RIGHT_45=1352,20
PRIMER_LEFT_45_TM=60.434
PRIMER_RIGHT_45_TM=60.074
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=897
PRIMER_PAIR_46_PENALTY=1.510300
PRIMER_LEFT_46_PENALTY=0.442948
PRIMER_RIGHT_46_PENALTY=1.067353
PRIMER_LEFT_46_SEQUENCE=AAGGGGATTAAGCTTCGGTG
PRIMER_RIGHT_46_SEQUENCE=GGTCCAAATCGATAAGGGTGT
PRIMER_LEFT_46=233,20
PRIMER_RIGHT_46=1212,21
PRIMER_LEFT_46_TM=60.443
PRIMER_RIGHT_46_TM=60.067
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=980
PRIMER_PAIR_47_PENALTY=1.513833
PRIMER_LEFT_47_PENALTY=0.415156
PRIMER_RIGHT_47_PENALTY=1.098677
PRIMER_LEFT_47_SEQUENCE=TAATGTCGTTGTTGGCCTTG
PRIMER_RIGHT_47_SEQUENCE=GAAAGGAGCCAAAAGCAACC
PRIMER_LEFT_47=886,20
PRIMER_RIGHT_47=1328,20
PRIMER_LEFT_47_TM=59.585
PRIMER_RIGHT_47_TM=61.099
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=443
PRIMER_PAIR_48_PENALTY=1.522677
PRIMER_LEFT_48_PENALTY=1.449063
PRIMER_RIGHT_48_PENALTY=0.073614
PRIMER_LEFT_48_SEQUENCE=GTGCATCCGAACAATGGAAG
PRIMER_RIGHT_48_SEQUENCE=TCTACAATTTGACGCCTCCC
PRIMER_LEFT_48=388,20
PRIMER_RIGHT_48=1352,20
PRIMER_LEFT_48_TM=61.449
PRIMER_RIGHT_48_TM=60.074
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=965
PRIMER_PAIR_49_PENALTY=1.527158
PRIMER_LEFT_49_PENALTY=0.428481
PRIMER_RIGHT_49_PENALTY=1.098677
PRIMER_LEFT_49_SEQUENCE=TGCTCACGGTACACAAATCC
PRIMER_RIGHT_49_SEQUENCE=GAAAGGAGCCAAAAGCAACC
PRIMER_LEFT_49=457,20
PRIMER_RIGHT_49=1328,20
PRIMER_LEFT_49_TM=59.572
PRIMER_RIGHT_49_TM=61.099
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=872
=
SEQUENCE_ID=MAGIC.287_3_21435482_21435731_po-0_hi-0_132540_21335_TRUE
SEQUENCE_TARGET=950,349
SEQUENCE_TEMPLATE=CTNGAGAGAGNAAATNAACATTGAAGAAGGGATTTGTGTGTGCTCTCTCTCTTGTGTTTTNTTGTCNTCNTCTTCTTCTTCTGCTTTTTTCTACAGTTTCCTTCTTTTTTTAACTCNGTGAAATANAAAAANAANAGAGGCACTGTTGCATNNNNNATCGANGTGGGGTCCATTTATAAATAAAAACACGCNAAGACATACTTCTCTNNGTTTTGAAATACCCTACANTACNCACGTGTGGGAGACGACGGTNCTGCGTAATCTTTCAACGGTGGTCAAATTTTACAAAATTATAATAATCCCTCTTATTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNACTATTGCATAATTGTGCAATTGATCTGGCCACTCAAAAGAAATGAAAAACACTATGGAATTGAAGAAGTCATTGGATTCCTCATCCTCGAGTGTTTTGGGTGAAGCATACGTACATTTATACAAAAGCCGTNTTTTGCTTTTTGCTGGACCCCGTTACTATTTTTTGTTAANTTTTCTGGTCAATAACGAAATAAATAAGTTAGGGGGTCCCATTTCGTTTTATCTTGTTNTCTGTCTACATAAGTNTTTTTANTTTATCTTTCTTTCNTTCAAAATTTACCTTAAACGTCTAAATCTCTATCACGANNNNNNNTCATAATTCCTACATTTATGTGTTTTATCTATCTAGACATGTGCATGCTTTTTTTACTTGATNTAGGANTTTTTTATNTTCGTTTATCCAAACAAACAAATANATGGTCACTGCTTTTGCTTNTTAACAAGTTTTTNTTTNCTGTCTANGTAAAAGCCATCATGATNNNTTTAGGTGAATNNNAAGANNNTAACTNTGNGTAAAATTNCAAAATGTATANTATCAAATGGATATAGATANACGANAAGTTTTAAACGCGTTAGCGAANTCTTNTTNTNGNTTTGNTATTTTAGTTTATTAGGTANAAAATGGAAATCATCCACANTTCGGAGCAATTGTTACAANAATGNGATTATCTTTCTACCGACTAATGTTGGGTAGACATAATTAGTTTACAAAATGTTCNTTTTCCATTTGTCACCAAAGTTTTCTTCATTATTTTNTGAAACAAGANTTGGGCAGTTGGACATATATACTATATTANTAGTTAAATTGCATATGTTTGATCTGTTGATNTGTATATTCAGTTCTNNAANTANATAACAAGNACTAACTATATTTCANTATTTGTATATAATCATTTAGTATCGAAANGGTCTCNNGGTCGTNGCACCTTTANTTGCTGAGAGGGNCGTCTTTTACTTTTCGTTCTAGCTTTATGATGTGCCACCGAGTAGTTANGAGAATGCTTCTGCTNCAAGTATTTTGACATAATTTAATCAAAAGTATTNGCNACTTTACACGGACAATAATCTAATTTTATAATTAGAGTAAGATAGAAATTAAAGAAGNTATTGGTAGTCGTTGCAAATGACACNTGTNGATCATGCTTTGCTCGCATTTGTATAGCTTCGACTTGAAGGATTTCAGTCCACCGTACGTTTACCACGATCGAGTCACCCANTACGNTCNNNNNTNNNANTNNNATTTGATAAGTGTAATAAGAAGACNANTNATNNNNAAAAAAGAAGAANACAAATTTGCTAAGATGACANTTTGTAATCCANATCTTATCACATCTACAAGAGAGATATATACNACTCTTTTTCTTAAGAGATGATTAATGCGTTAAANAANTTTAACCAGTTANCAAAAATATGTAAATCTTTTCTCCAGAGGTTTAAAGACAACGTCACGGTAATTGTTTCGTNNAAANNNGACTATTTTAAACNGTANGGTTCATGGTCACTTTTTGTGTATTTANATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.868611
PRIMER_LEFT_0_PENALTY=0.033680
PRIMER_RIGHT_0_PENALTY=0.834931
PRIMER_LEFT_0_SEQUENCE=ATCCTCGAGTGTTTTGGGTG
PRIMER_RIGHT_0_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_0=424,20
PRIMER_RIGHT_0=1334,20
PRIMER_LEFT_0_TM=59.966
PRIMER_RIGHT_0_TM=59.165
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=911
PRIMER_PAIR_1_PENALTY=0.868611
PRIMER_LEFT_1_PENALTY=0.033680
PRIMER_RIGHT_1_PENALTY=0.834931
PRIMER_LEFT_1_SEQUENCE=CATCCTCGAGTGTTTTGGGT
PRIMER_RIGHT_1_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_1=423,20
PRIMER_RIGHT_1=1334,20
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.165
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=912
PRIMER_PAIR_2_PENALTY=1.031845
PRIMER_LEFT_2_PENALTY=0.033680
PRIMER_RIGHT_2_PENALTY=0.998165
PRIMER_LEFT_2_SEQUENCE=ATCCTCGAGTGTTTTGGGTG
PRIMER_RIGHT_2_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_2=424,20
PRIMER_RIGHT_2=1330,20
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.002
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=907
PRIMER_PAIR_3_PENALTY=1.031845
PRIMER_LEFT_3_PENALTY=0.033680
PRIMER_RIGHT_3_PENALTY=0.998165
PRIMER_LEFT_3_SEQUENCE=CATCCTCGAGTGTTTTGGGT
PRIMER_RIGHT_3_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_3=423,20
PRIMER_RIGHT_3=1330,20
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.002
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=908
PRIMER_PAIR_4_PENALTY=1.587983
PRIMER_LEFT_4_PENALTY=0.753052
PRIMER_RIGHT_4_PENALTY=0.834931
PRIMER_LEFT_4_SEQUENCE=TGGCCACTCAAAAGAAATGA
PRIMER_RIGHT_4_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_4=367,20
PRIMER_RIGHT_4=1334,20
PRIMER_LEFT_4_TM=59.247
PRIMER_RIGHT_4_TM=59.165
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=968
PRIMER_PAIR_5_PENALTY=1.629376
PRIMER_LEFT_5_PENALTY=0.631211
PRIMER_RIGHT_5_PENALTY=0.998165
PRIMER_LEFT_5_SEQUENCE=TGATCTGGCCACTCAAAAGA
PRIMER_RIGHT_5_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_5=362,20
PRIMER_RIGHT_5=1330,20
PRIMER_LEFT_5_TM=59.369
PRIMER_RIGHT_5_TM=59.002
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.2000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=969
PRIMER_PAIR_6_PENALTY=1.751217
PRIMER_LEFT_6_PENALTY=0.753052
PRIMER_RIGHT_6_PENALTY=0.998165
PRIMER_LEFT_6_SEQUENCE=TGGCCACTCAAAAGAAATGA
PRIMER_RIGHT_6_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_6=367,20
PRIMER_RIGHT_6=1330,20
PRIMER_LEFT_6_TM=59.247
PRIMER_RIGHT_6_TM=59.002
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=6.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=964
PRIMER_PAIR_7_PENALTY=1.824257
PRIMER_LEFT_7_PENALTY=0.033680
PRIMER_RIGHT_7_PENALTY=1.790576
PRIMER_LEFT_7_SEQUENCE=ATCCTCGAGTGTTTTGGGTG
PRIMER_RIGHT_7_SEQUENCE=TAACTACTCGGTGGCACATCA
PRIMER_LEFT_7=424,20
PRIMER_RIGHT_7=1335,21
PRIMER_LEFT_7_TM=59.966
PRIMER_RIGHT_7_TM=59.209
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=912
PRIMER_PAIR_8_PENALTY=1.824257
PRIMER_LEFT_8_PENALTY=0.033680
PRIMER_RIGHT_8_PENALTY=1.790576
PRIMER_LEFT_8_SEQUENCE=CATCCTCGAGTGTTTTGGGT
PRIMER_RIGHT_8_SEQUENCE=TAACTACTCGGTGGCACATCA
PRIMER_LEFT_8=423,20
PRIMER_RIGHT_8=1335,21
PRIMER_LEFT_8_TM=59.966
PRIMER_RIGHT_8_TM=59.209
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=913
PRIMER_PAIR_9_PENALTY=1.869337
PRIMER_LEFT_9_PENALTY=1.034406
PRIMER_RIGHT_9_PENALTY=0.834931
PRIMER_LEFT_9_SEQUENCE=TCATCCTCGAGTGTTTTGGG
PRIMER_RIGHT_9_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_9=422,20
PRIMER_RIGHT_9=1334,20
PRIMER_LEFT_9_TM=61.034
PRIMER_RIGHT_9_TM=59.165
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=10.0000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=913
PRIMER_PAIR_10_PENALTY=1.898756
PRIMER_LEFT_10_PENALTY=1.063825
PRIMER_RIGHT_10_PENALTY=0.834931
PRIMER_LEFT_10_SEQUENCE=CCTCGAGTGTTTTGGGTGAA
PRIMER_RIGHT_10_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_10=426,20
PRIMER_RIGHT_10=1334,20
PRIMER_LEFT_10_TM=61.064
PRIMER_RIGHT_10_TM=59.165
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=909
PRIMER_PAIR_11_PENALTY=1.982155
PRIMER_LEFT_11_PENALTY=0.033680
PRIMER_RIGHT_11_PENALTY=1.948474
PRIMER_LEFT_11_SEQUENCE=ATCCTCGAGTGTTTTGGGTG
PRIMER_RIGHT_11_SEQUENCE=TACTCGGTGGCACATCATAAA
PRIMER_LEFT_11=424,20
PRIMER_RIGHT_11=1331,21
PRIMER_LEFT_11_TM=59.966
PRIMER_RIGHT_11_TM=59.052
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=908
PRIMER_PAIR_12_PENALTY=1.982155
PRIMER_LEFT_12_PENALTY=0.033680
PRIMER_RIGHT_12_PENALTY=1.948474
PRIMER_LEFT_12_SEQUENCE=CATCCTCGAGTGTTTTGGGT
PRIMER_RIGHT_12_SEQUENCE=TACTCGGTGGCACATCATAAA
PRIMER_LEFT_12=423,20
PRIMER_RIGHT_12=1331,21
PRIMER_LEFT_12_TM=59.966
PRIMER_RIGHT_12_TM=59.052
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=909
PRIMER_PAIR_13_PENALTY=2.032571
PRIMER_LEFT_13_PENALTY=1.034406
PRIMER_RIGHT_13_PENALTY=0.998165
PRIMER_LEFT_13_SEQUENCE=TCATCCTCGAGTGTTTTGGG
PRIMER_RIGHT_13_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_13=422,20
PRIMER_RIGHT_13=1330,20
PRIMER_LEFT_13_TM=61.034
PRIMER_RIGHT_13_TM=59.002
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=6.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=909
PRIMER_PAIR_14_PENALTY=2.051526
PRIMER_LEFT_14_PENALTY=0.033680
PRIMER_RIGHT_14_PENALTY=2.017846
PRIMER_LEFT_14_SEQUENCE=ATCCTCGAGTGTTTTGGGTG
PRIMER_RIGHT_14_SEQUENCE=CTACTCGGTGGCACATCATAAA
PRIMER_LEFT_14=424,20
PRIMER_RIGHT_14=1332,22
PRIMER_LEFT_14_TM=59.966
PRIMER_RIGHT_14_TM=60.018
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=6.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=909
PRIMER_PAIR_15_PENALTY=2.051526
PRIMER_LEFT_15_PENALTY=0.033680
PRIMER_RIGHT_15_PENALTY=2.017846
PRIMER_LEFT_15_SEQUENCE=CATCCTCGAGTGTTTTGGGT
PRIMER_RIGHT_15_SEQUENCE=CTACTCGGTGGCACATCATAAA
PRIMER_LEFT_15=423,20
PRIMER_RIGHT_15=1332,22
PRIMER_LEFT_15_TM=59.966
PRIMER_RIGHT_15_TM=60.018
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.455
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=6.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=910
PRIMER_PAIR_16_PENALTY=2.061990
PRIMER_LEFT_16_PENALTY=1.063825
PRIMER_RIGHT_16_PENALTY=0.998165
PRIMER_LEFT_16_SEQUENCE=CCTCGAGTGTTTTGGGTGAA
PRIMER_RIGHT_16_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_16=426,20
PRIMER_RIGHT_16=1330,20
PRIMER_LEFT_16_TM=61.064
PRIMER_RIGHT_16_TM=59.002
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=905
PRIMER_PAIR_17_PENALTY=2.069147
PRIMER_LEFT_17_PENALTY=1.234216
PRIMER_RIGHT_17_PENALTY=0.834931
PRIMER_LEFT_17_SEQUENCE=CTGGCCACTCAAAAGAAATGA
PRIMER_RIGHT_17_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_17=366,21
PRIMER_RIGHT_17=1334,20
PRIMER_LEFT_17_TM=60.234
PRIMER_RIGHT_17_TM=59.165
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=969
PRIMER_PAIR_18_PENALTY=2.069147
PRIMER_LEFT_18_PENALTY=1.234216
PRIMER_RIGHT_18_PENALTY=0.834931
PRIMER_LEFT_18_SEQUENCE=TCTGGCCACTCAAAAGAAATG
PRIMER_RIGHT_18_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_18=365,21
PRIMER_RIGHT_18=1334,20
PRIMER_LEFT_18_TM=60.234
PRIMER_RIGHT_18_TM=59.165
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=970
PRIMER_PAIR_19_PENALTY=2.232381
PRIMER_LEFT_19_PENALTY=1.234216
PRIMER_RIGHT_19_PENALTY=0.998165
PRIMER_LEFT_19_SEQUENCE=CTGGCCACTCAAAAGAAATGA
PRIMER_RIGHT_19_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_19=366,21
PRIMER_RIGHT_19=1330,20
PRIMER_LEFT_19_TM=60.234
PRIMER_RIGHT_19_TM=59.002
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=6.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=965
PRIMER_PAIR_20_PENALTY=2.232381
PRIMER_LEFT_20_PENALTY=1.234216
PRIMER_RIGHT_20_PENALTY=0.998165
PRIMER_LEFT_20_SEQUENCE=TCTGGCCACTCAAAAGAAATG
PRIMER_RIGHT_20_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_20=365,21
PRIMER_RIGHT_20=1330,20
PRIMER_LEFT_20_TM=60.234
PRIMER_RIGHT_20_TM=59.002
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=6.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=966
PRIMER_PAIR_21_PENALTY=2.301694
PRIMER_LEFT_21_PENALTY=1.773654
PRIMER_RIGHT_21_PENALTY=0.528040
PRIMER_LEFT_21_SEQUENCE=GGGTCCCATTTCGTTTTATC
PRIMER_RIGHT_21_SEQUENCE=ATACAAATGCGAGCAAAGCA
PRIMER_LEFT_21=547,20
PRIMER_RIGHT_21=1502,20
PRIMER_LEFT_21_TM=58.226
PRIMER_RIGHT_21_TM=59.472
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=5.9000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=956
PRIMER_PAIR_22_PENALTY=2.336469
PRIMER_LEFT_22_PENALTY=1.808429
PRIMER_RIGHT_22_PENALTY=0.528040
PRIMER_LEFT_22_SEQUENCE=GGGTCCCATTTCGTTTTATCT
PRIMER_RIGHT_22_SEQUENCE=ATACAAATGCGAGCAAAGCA
PRIMER_LEFT_22=547,21
PRIMER_RIGHT_22=1502,20
PRIMER_LEFT_22_TM=59.192
PRIMER_RIGHT_22_TM=59.472
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.6000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=956
PRIMER_PAIR_23_PENALTY=2.449746
PRIMER_LEFT_23_PENALTY=1.614815
PRIMER_RIGHT_23_PENALTY=0.834931
PRIMER_LEFT_23_SEQUENCE=GCTGGACCCCGTTACTATTTT
PRIMER_RIGHT_23_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_23=485,21
PRIMER_RIGHT_23=1334,20
PRIMER_LEFT_23_TM=59.385
PRIMER_RIGHT_23_TM=59.165
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.2000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=850
PRIMER_PAIR_24_PENALTY=2.470555
PRIMER_LEFT_24_PENALTY=1.635624
PRIMER_RIGHT_24_PENALTY=0.834931
PRIMER_LEFT_24_SEQUENCE=TCCTCGAGTGTTTTGGGTGA
PRIMER_RIGHT_24_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_24=425,20
PRIMER_RIGHT_24=1334,20
PRIMER_LEFT_24_TM=61.636
PRIMER_RIGHT_24_TM=59.165
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=910
PRIMER_PAIR_25_PENALTY=2.483331
PRIMER_LEFT_25_PENALTY=1.648400
PRIMER_RIGHT_25_PENALTY=0.834931
PRIMER_LEFT_25_SEQUENCE=CTGGCCACTCAAAAGAAATG
PRIMER_RIGHT_25_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_25=366,20
PRIMER_RIGHT_25=1334,20
PRIMER_LEFT_25_TM=58.352
PRIMER_RIGHT_25_TM=59.165
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.2000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=969
PRIMER_PAIR_26_PENALTY=2.493045
PRIMER_LEFT_26_PENALTY=1.658114
PRIMER_RIGHT_26_PENALTY=0.834931
PRIMER_LEFT_26_SEQUENCE=CTCGAGTGTTTTGGGTGAAG
PRIMER_RIGHT_26_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_26=427,20
PRIMER_RIGHT_26=1334,20
PRIMER_LEFT_26_TM=58.342
PRIMER_RIGHT_26_TM=59.165
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=908
PRIMER_PAIR_27_PENALTY=2.532636
PRIMER_LEFT_27_PENALTY=1.697705
PRIMER_RIGHT_27_PENALTY=0.834931
PRIMER_LEFT_27_SEQUENCE=GATCTGGCCACTCAAAAGAAA
PRIMER_RIGHT_27_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_27=363,21
PRIMER_RIGHT_27=1334,20
PRIMER_LEFT_27_TM=59.302
PRIMER_RIGHT_27_TM=59.165
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=972
PRIMER_PAIR_28_PENALTY=2.543628
PRIMER_LEFT_28_PENALTY=0.753052
PRIMER_RIGHT_28_PENALTY=1.790576
PRIMER_LEFT_28_SEQUENCE=TGGCCACTCAAAAGAAATGA
PRIMER_RIGHT_28_SEQUENCE=TAACTACTCGGTGGCACATCA
PRIMER_LEFT_28=367,20
PRIMER_RIGHT_28=1335,21
PRIMER_LEFT_28_TM=59.247
PRIMER_RIGHT_28_TM=59.209
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=969
PRIMER_PAIR_29_PENALTY=2.548546
PRIMER_LEFT_29_PENALTY=1.713615
PRIMER_RIGHT_29_PENALTY=0.834931
PRIMER_LEFT_29_SEQUENCE=CTCATCCTCGAGTGTTTTGG
PRIMER_RIGHT_29_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_29=421,20
PRIMER_RIGHT_29=1334,20
PRIMER_LEFT_29_TM=58.286
PRIMER_RIGHT_29_TM=59.165
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.8000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=914
PRIMER_PAIR_30_PENALTY=2.561366
PRIMER_LEFT_30_PENALTY=1.726435
PRIMER_RIGHT_30_PENALTY=0.834931
PRIMER_LEFT_30_SEQUENCE=TGCTGGACCCCGTTACTATTT
PRIMER_RIGHT_30_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_30=484,21
PRIMER_RIGHT_30=1334,20
PRIMER_LEFT_30_TM=60.726
PRIMER_RIGHT_30_TM=59.165
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.2000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=851
PRIMER_PAIR_31_PENALTY=2.579685
PRIMER_LEFT_31_PENALTY=0.631211
PRIMER_RIGHT_31_PENALTY=1.948474
PRIMER_LEFT_31_SEQUENCE=TGATCTGGCCACTCAAAAGA
PRIMER_RIGHT_31_SEQUENCE=TACTCGGTGGCACATCATAAA
PRIMER_LEFT_31=362,20
PRIMER_RIGHT_31=1331,21
PRIMER_LEFT_31_TM=59.369
PRIMER_RIGHT_31_TM=59.052
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.2000
PRIMER_PAIR_31_COMPL_ANY=7.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=970
PRIMER_PAIR_32_PENALTY=2.601927
PRIMER_LEFT_32_PENALTY=1.766996
PRIMER_RIGHT_32_PENALTY=0.834931
PRIMER_LEFT_32_SEQUENCE=TTTTGCTGGACCCCGTTACT
PRIMER_RIGHT_32_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_32=481,20
PRIMER_RIGHT_32=1334,20
PRIMER_LEFT_32_TM=61.767
PRIMER_RIGHT_32_TM=59.165
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=5.7000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=854
PRIMER_PAIR_33_PENALTY=2.608585
PRIMER_LEFT_33_PENALTY=1.773654
PRIMER_RIGHT_33_PENALTY=0.834931
PRIMER_LEFT_33_SEQUENCE=GGGTCCCATTTCGTTTTATC
PRIMER_RIGHT_33_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_33=547,20
PRIMER_RIGHT_33=1334,20
PRIMER_LEFT_33_TM=58.226
PRIMER_RIGHT_33_TM=59.165
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=5.9000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=788
PRIMER_PAIR_34_PENALTY=2.612980
PRIMER_LEFT_34_PENALTY=1.614815
PRIMER_RIGHT_34_PENALTY=0.998165
PRIMER_LEFT_34_SEQUENCE=GCTGGACCCCGTTACTATTTT
PRIMER_RIGHT_34_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_34=485,21
PRIMER_RIGHT_34=1330,20
PRIMER_LEFT_34_TM=59.385
PRIMER_RIGHT_34_TM=59.002
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.2000
PRIMER_RIGHT_34_END_STABILITY=6.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=846
PRIMER_PAIR_35_PENALTY=2.633789
PRIMER_LEFT_35_PENALTY=1.635624
PRIMER_RIGHT_35_PENALTY=0.998165
PRIMER_LEFT_35_SEQUENCE=TCCTCGAGTGTTTTGGGTGA
PRIMER_RIGHT_35_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_35=425,20
PRIMER_RIGHT_35=1330,20
PRIMER_LEFT_35_TM=61.636
PRIMER_RIGHT_35_TM=59.002
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=906
PRIMER_PAIR_36_PENALTY=2.643360
PRIMER_LEFT_36_PENALTY=1.808429
PRIMER_RIGHT_36_PENALTY=0.834931
PRIMER_LEFT_36_SEQUENCE=GGGTCCCATTTCGTTTTATCT
PRIMER_RIGHT_36_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_36=547,21
PRIMER_RIGHT_36=1334,20
PRIMER_LEFT_36_TM=59.192
PRIMER_RIGHT_36_TM=59.165
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=5.6000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=788
PRIMER_PAIR_37_PENALTY=2.646565
PRIMER_LEFT_37_PENALTY=1.648400
PRIMER_RIGHT_37_PENALTY=0.998165
PRIMER_LEFT_37_SEQUENCE=CTGGCCACTCAAAAGAAATG
PRIMER_RIGHT_37_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_37=366,20
PRIMER_RIGHT_37=1330,20
PRIMER_LEFT_37_TM=58.352
PRIMER_RIGHT_37_TM=59.002
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.2000
PRIMER_RIGHT_37_END_STABILITY=6.2000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=965
PRIMER_PAIR_38_PENALTY=2.649057
PRIMER_LEFT_38_PENALTY=0.631211
PRIMER_RIGHT_38_PENALTY=2.017846
PRIMER_LEFT_38_SEQUENCE=TGATCTGGCCACTCAAAAGA
PRIMER_RIGHT_38_SEQUENCE=CTACTCGGTGGCACATCATAAA
PRIMER_LEFT_38=362,20
PRIMER_RIGHT_38=1332,22
PRIMER_LEFT_38_TM=59.369
PRIMER_RIGHT_38_TM=60.018
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.2000
PRIMER_PAIR_38_COMPL_ANY=7.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=971
PRIMER_PAIR_39_PENALTY=2.656278
PRIMER_LEFT_39_PENALTY=1.658114
PRIMER_RIGHT_39_PENALTY=0.998165
PRIMER_LEFT_39_SEQUENCE=CTCGAGTGTTTTGGGTGAAG
PRIMER_RIGHT_39_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_39=427,20
PRIMER_RIGHT_39=1330,20
PRIMER_LEFT_39_TM=58.342
PRIMER_RIGHT_39_TM=59.002
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=904
PRIMER_PAIR_40_PENALTY=2.695870
PRIMER_LEFT_40_PENALTY=1.697705
PRIMER_RIGHT_40_PENALTY=0.998165
PRIMER_LEFT_40_SEQUENCE=GATCTGGCCACTCAAAAGAAA
PRIMER_RIGHT_40_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_40=363,21
PRIMER_RIGHT_40=1330,20
PRIMER_LEFT_40_TM=59.302
PRIMER_RIGHT_40_TM=59.002
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.2000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=968
PRIMER_PAIR_41_PENALTY=2.701527
PRIMER_LEFT_41_PENALTY=0.753052
PRIMER_RIGHT_41_PENALTY=1.948474
PRIMER_LEFT_41_SEQUENCE=TGGCCACTCAAAAGAAATGA
PRIMER_RIGHT_41_SEQUENCE=TACTCGGTGGCACATCATAAA
PRIMER_LEFT_41=367,20
PRIMER_RIGHT_41=1331,21
PRIMER_LEFT_41_TM=59.247
PRIMER_RIGHT_41_TM=59.052
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=6.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=965
PRIMER_PAIR_42_PENALTY=2.707454
PRIMER_LEFT_42_PENALTY=2.179414
PRIMER_RIGHT_42_PENALTY=0.528040
PRIMER_LEFT_42_SEQUENCE=GTTAGGGGGTCCCATTTCGT
PRIMER_RIGHT_42_SEQUENCE=ATACAAATGCGAGCAAAGCA
PRIMER_LEFT_42=541,20
PRIMER_RIGHT_42=1502,20
PRIMER_LEFT_42_TM=62.179
PRIMER_RIGHT_42_TM=59.472
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=962
PRIMER_PAIR_43_PENALTY=2.711780
PRIMER_LEFT_43_PENALTY=1.713615
PRIMER_RIGHT_43_PENALTY=0.998165
PRIMER_LEFT_43_SEQUENCE=CTCATCCTCGAGTGTTTTGG
PRIMER_RIGHT_43_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_43=421,20
PRIMER_RIGHT_43=1330,20
PRIMER_LEFT_43_TM=58.286
PRIMER_RIGHT_43_TM=59.002
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.8000
PRIMER_RIGHT_43_END_STABILITY=6.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=910
PRIMER_PAIR_44_PENALTY=2.724600
PRIMER_LEFT_44_PENALTY=1.726435
PRIMER_RIGHT_44_PENALTY=0.998165
PRIMER_LEFT_44_SEQUENCE=TGCTGGACCCCGTTACTATTT
PRIMER_RIGHT_44_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_44=484,21
PRIMER_RIGHT_44=1330,20
PRIMER_LEFT_44_TM=60.726
PRIMER_RIGHT_44_TM=59.002
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.2000
PRIMER_RIGHT_44_END_STABILITY=6.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=847
PRIMER_PAIR_45_PENALTY=2.758475
PRIMER_LEFT_45_PENALTY=1.760311
PRIMER_RIGHT_45_PENALTY=0.998165
PRIMER_LEFT_45_SEQUENCE=TGATCTGGCCACTCAAAAGAA
PRIMER_RIGHT_45_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_45=362,21
PRIMER_RIGHT_45=1330,20
PRIMER_LEFT_45_TM=60.760
PRIMER_RIGHT_45_TM=59.002
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.2000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=969
PRIMER_PAIR_46_PENALTY=2.763144
PRIMER_LEFT_46_PENALTY=1.928213
PRIMER_RIGHT_46_PENALTY=0.834931
PRIMER_LEFT_46_SEQUENCE=GCTGGACCCCGTTACTATTT
PRIMER_RIGHT_46_SEQUENCE=AACTACTCGGTGGCACATCA
PRIMER_LEFT_46=485,20
PRIMER_RIGHT_46=1334,20
PRIMER_LEFT_46_TM=58.072
PRIMER_RIGHT_46_TM=59.165
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.2000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=850
PRIMER_PAIR_47_PENALTY=2.770898
PRIMER_LEFT_47_PENALTY=0.753052
PRIMER_RIGHT_47_PENALTY=2.017846
PRIMER_LEFT_47_SEQUENCE=TGGCCACTCAAAAGAAATGA
PRIMER_RIGHT_47_SEQUENCE=CTACTCGGTGGCACATCATAAA
PRIMER_LEFT_47=367,20
PRIMER_RIGHT_47=1332,22
PRIMER_LEFT_47_TM=59.247
PRIMER_RIGHT_47_TM=60.018
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=6.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=966
PRIMER_PAIR_48_PENALTY=2.779475
PRIMER_LEFT_48_PENALTY=1.614815
PRIMER_RIGHT_48_PENALTY=1.164660
PRIMER_LEFT_48_SEQUENCE=GCTGGACCCCGTTACTATTTT
PRIMER_RIGHT_48_SEQUENCE=TGTCATTTGCAACGACTACCA
PRIMER_LEFT_48=485,21
PRIMER_RIGHT_48=1471,21
PRIMER_LEFT_48_TM=59.385
PRIMER_RIGHT_48_TM=60.165
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.2000
PRIMER_RIGHT_48_END_STABILITY=7.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=987
PRIMER_PAIR_49_PENALTY=2.806594
PRIMER_LEFT_49_PENALTY=1.808429
PRIMER_RIGHT_49_PENALTY=0.998165
PRIMER_LEFT_49_SEQUENCE=GGGTCCCATTTCGTTTTATCT
PRIMER_RIGHT_49_SEQUENCE=ACTCGGTGGCACATCATAAA
PRIMER_LEFT_49=547,21
PRIMER_RIGHT_49=1330,20
PRIMER_LEFT_49_TM=59.192
PRIMER_RIGHT_49_TM=59.002
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.6000
PRIMER_RIGHT_49_END_STABILITY=6.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=784
=
SEQUENCE_ID=MAGIC.175_3_14532212_14532943_wil-2_hi-0_31701_2734_TRUE
SEQUENCE_TARGET=950,831
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_10076898_10076906_zu-0_hi-0_1136_143392_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=TTTCATATTCATTCTAAAATTTTTCATTTGAGNAAACCNTCTCTTTTTTCNTNTNANNNTATTCTATTATCTTNTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_8543457_8543712_wu-0_oy-0_15432_107858_TRUE
SEQUENCE_TARGET=950,355
SEQUENCE_TEMPLATE=TACATTATTGGTGATAGTTTTCATAACCTAAGTCCTTCATAAAATCTTTGCAAAATCCCAAGAAATGNTGACAGGATGCAGATTTTATGCCAAAATAAAGAACCAACTGATATATCAAATCATTTTCAGGCAATATGAATTACCAATGACTCCATAGAAGAGGAACTATTTTGTCTTTATCACAATAATGCCNNNNNNNNNTACTGGTTAGCATAGATATAATTGATCTTGACATTTAAAGGTTATACTTCACGTATACTTTAAGTTTATGCCAGCGTATACTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAAAATNTTCTATCTAGAAATCAGCTAGCCTTTCAATAGTCANCCTGTAATTAAGGACCTTTAAAACTCCGACTAAGCCGGGTCAACCCGCAAAGTCGCNAAAAAAATCGTATCGGATAACAAAATCAAAAGAAGTGGTCTCATNNNNNNNNNNNNNNNNNNNNTTCGATTGTGCNACGTGTTTGAATCGTATCGAATGTTCCTACTATGACTCATANATATAGACAATAACACGTCATCAGTCAAAGACCAAAAGCCTTGTACCACGTGTACTTTTCGTACTACATATTAGCCCCACTGATTCTCTTTTCTGTTCNCAATTTTATTAAGATATTTTTAGTAATCTTTGTCGGNATTCTATTTTAGCTAAAAGGAAAANGTGACACGTGGTAGGAGGTTAAGACACGTGGNGTNAGNGNAGACAAATGTCACGTGACTTTGTGACAGATCACGTGATTAGGTTGCTCTCTTTGGACTTGTTCGTTTTAGCTCCTTTATTGAAAACCGGCTACTNTACTGCAAACTTTCTCACTATACACAATAATGTCTGACGANTGATGCATATATANGCTTCTAACAAAGCATTTTAANNTGGATTACATGAATTTTACAATATTAGATTANATNTTATAATACGTACATATATTTTTACANATTTTNAGAGTNTGTCTTTGTTTCACATTCACAGTAATCNGTTTTATATATTTAACTACATTATTCGATTAACACATTACACATGTATTCACGGTTTTCATGGTGTGAAATTACAATATNTTTATTTAGAATAAAATCTAATCAAAGATTTCATANGAAAAAGAAAATAATAATTAAGGGATTAATTGTTTATGGGCTCACGTAGTAGTCATGATGGTGAGTATAAATCGTACAAGATATTAAATNTTTCATTTAATGGNCGGTCATGCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_11956936_11956948_col-0_ct-1_78_814791_TRUE
SEQUENCE_TARGET=950,112
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNAACGTTCTATAATTTNTATNNCTTTNAATAATTTATTNAATTAATACTTAAATAATAAAGGGTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTNAANNANATAAATGGTATGTCTTTCACTTTGNACGGAGTATCCCNATGTGGGGTACATAAATATTAAGAGAAAAAAAGAGATCAAGCTTACTAGTTTAAGCGTTAATAATTATACTGACTGACCAATCTACTTGTTCATGTAGCTCTAATGTTATTAATTANTATATAGTCTTAGATTGATCTAATTAATGTCTGTGTATGATGTCACTTTGTGATGATGGAAGTCATGAGAGCTTCCATTACTTAACTCTCTCTCTCTCTNTCTATNTATNTTTACGGTCATGTATAGTGTAATCANNAATAACAAGAAAAAGCCAAGTTCAAAGCACNATACTATTAGTTAAAGACAAATGCGATAATGATACGATTCTGAGCATTACTTGAGTCNCAATATATAGTCACACCCTCATCAAATTCTTTNTATCTTACCAACTTCTNAAAAAAAANNAAAAANNNAAAAGACAAGTAAATACATANATAAATATACACNCACACACACACACATATATTGATATACTTATAATNATAAATCATGGTAAAGTTTGANACAAAACTATGGAAGGCTGTGCTTATGATGTCCATGATCAACATTGGTTTAAGTGTTGTAAACGTTATGTTCAAGAAGATGATTGATGAAGGACTTAACCGTATGGTCGCGACCACTTACCGACTTGCTGTAGGAACTCTGTTCTTNATACCATTCGCGATTTTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNTTNNNNNNNNTNTNNNNNGNTNNNTGTNTTNTTNNTTNTNNNNNTTNNANTTNTNNTTNAANTNTNNNNTTNAATNATAANNGGATGTCTTGTTTATACNANTNTCTAGTTGAATGTTGTTTTCAAACTCTTTTCTCCTGTGAATATGTNATTATATATTCGTATTCCATATGAAAGATTTTGNNNNNNNNNNNNNTTGTTCTTTTTTTNNNNNTCTCTTTTNGTAGACATAACAGGCCAAAACTCACGGGTAGGATCTTGTGTTCACTCTTCTTTAGCGCTCTTCTGGGGTATGTTCATTTTATTAGACTGTTCANTTATAATAAATGTTTACAAATAAGTTTTGAGTTGTTTNTTNTGATCTTGATCGTTNNTATATTTGCAGGACAAGTTTGGTGCAATACTTCTTTCTTATTGGACTAGAGTATACATCTTCAACNTTTTCCTTAGCNTTTAGCAACATGGTTCCTTCNGTTACNTTTGCNTTGGCTCTCGTCTTTAGGTANTNAAAAATCGATCAAACNCATTTGNATTCTTCATATATATNCTCTAGTATTATCNNAATNNTTNATAAACTANANGANTAGGCAAGAGACGCTGAACATCAAGAGCAACGTAGGAAGAGCCAAACTGCTAGGCACAATGATATGTATTTGTGGAGCTTTAGTGCTTACGCTTTATAAAGGAACCGCTTTAAGTCGAGAACANAGTACTCATATGNAAACACATACAAGAACTGATTCCACCGGTGCAATGACNCAAAAGTGGGCAATGGGTTCGATCATGCTGGTTATATCNATCATNATATGGTCATCTTGGTTCATTGTTCAAGCTAAAATAAGCNGANTTTATCCNTGCCAATACACGAGTACCACGATCCTCTCTTTCTTTGGCGTGATNCAATCTGCTTTGTTGAGTTTGATCTCGGAGAGGAGCACNTCTATGTGGGTCGTTAAAGACAAGTTCCAAGTTTTAGCTTTACTTTATTCGGTGAGTNATCCAATATACTATTTAATCAACCTACAAGATCTATATGTCAANATATATANAATTTTNTTTNAGNNNNNNNTTGTAAANAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.327749
PRIMER_LEFT_0_PENALTY=0.312496
PRIMER_RIGHT_0_PENALTY=0.015253
PRIMER_LEFT_0_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_0_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_0=814,20
PRIMER_RIGHT_0=1560,20
PRIMER_LEFT_0_TM=60.312
PRIMER_RIGHT_0_TM=60.015
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=747
PRIMER_PAIR_1_PENALTY=0.332263
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=0.019767
PRIMER_LEFT_1_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_1_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_1=814,20
PRIMER_RIGHT_1=1536,20
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=723
PRIMER_PAIR_2_PENALTY=0.337624
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_2_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_2=814,20
PRIMER_RIGHT_2=1224,20
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=411
PRIMER_PAIR_3_PENALTY=0.346176
PRIMER_LEFT_3_PENALTY=0.312496
PRIMER_RIGHT_3_PENALTY=0.033680
PRIMER_LEFT_3_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_3_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_3=814,20
PRIMER_RIGHT_3=1680,20
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=867
PRIMER_PAIR_4_PENALTY=0.346176
PRIMER_LEFT_4_PENALTY=0.312496
PRIMER_RIGHT_4_PENALTY=0.033680
PRIMER_LEFT_4_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_4_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_4=814,20
PRIMER_RIGHT_4=1681,20
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=868
PRIMER_PAIR_5_PENALTY=0.466519
PRIMER_LEFT_5_PENALTY=0.312496
PRIMER_RIGHT_5_PENALTY=0.154023
PRIMER_LEFT_5_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_5_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_5=814,20
PRIMER_RIGHT_5=1571,20
PRIMER_LEFT_5_TM=60.312
PRIMER_RIGHT_5_TM=60.154
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=758
PRIMER_PAIR_6_PENALTY=0.578933
PRIMER_LEFT_6_PENALTY=0.312496
PRIMER_RIGHT_6_PENALTY=0.266437
PRIMER_LEFT_6_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_6_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_6=814,20
PRIMER_RIGHT_6=1329,20
PRIMER_LEFT_6_TM=60.312
PRIMER_RIGHT_6_TM=59.734
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=516
PRIMER_PAIR_7_PENALTY=0.588245
PRIMER_LEFT_7_PENALTY=0.312496
PRIMER_RIGHT_7_PENALTY=0.275749
PRIMER_LEFT_7_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_7_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_7=814,20
PRIMER_RIGHT_7=1221,20
PRIMER_LEFT_7_TM=60.312
PRIMER_RIGHT_7_TM=59.724
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=408
PRIMER_PAIR_8_PENALTY=0.668069
PRIMER_LEFT_8_PENALTY=0.312496
PRIMER_RIGHT_8_PENALTY=0.355573
PRIMER_LEFT_8_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_8_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_8=814,20
PRIMER_RIGHT_8=1572,20
PRIMER_LEFT_8_TM=60.312
PRIMER_RIGHT_8_TM=59.644
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=759
PRIMER_PAIR_9_PENALTY=0.716170
PRIMER_LEFT_9_PENALTY=0.312496
PRIMER_RIGHT_9_PENALTY=0.403673
PRIMER_LEFT_9_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_9_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_9=814,20
PRIMER_RIGHT_9=1545,20
PRIMER_LEFT_9_TM=60.312
PRIMER_RIGHT_9_TM=59.596
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=732
PRIMER_PAIR_10_PENALTY=0.850580
PRIMER_LEFT_10_PENALTY=0.312496
PRIMER_RIGHT_10_PENALTY=0.538083
PRIMER_LEFT_10_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_10_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_10=814,20
PRIMER_RIGHT_10=1559,20
PRIMER_LEFT_10_TM=60.312
PRIMER_RIGHT_10_TM=60.538
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=746
PRIMER_PAIR_11_PENALTY=1.090076
PRIMER_LEFT_11_PENALTY=0.312496
PRIMER_RIGHT_11_PENALTY=0.777580
PRIMER_LEFT_11_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_11_SEQUENCE=GCCTAGCAGTTTGGCTCTTC
PRIMER_LEFT_11=814,20
PRIMER_RIGHT_11=1570,20
PRIMER_LEFT_11_TM=60.312
PRIMER_RIGHT_11_TM=59.222
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=757
PRIMER_PAIR_12_PENALTY=1.176120
PRIMER_LEFT_12_PENALTY=0.312496
PRIMER_RIGHT_12_PENALTY=0.863624
PRIMER_LEFT_12_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_12_SEQUENCE=TAACCAGCATGATCGAACCC
PRIMER_LEFT_12=814,20
PRIMER_RIGHT_12=1724,20
PRIMER_LEFT_12_TM=60.312
PRIMER_RIGHT_12_TM=60.864
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=911
PRIMER_PAIR_13_PENALTY=1.235854
PRIMER_LEFT_13_PENALTY=0.312496
PRIMER_RIGHT_13_PENALTY=0.923358
PRIMER_LEFT_13_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_13_SEQUENCE=CCTAGCAGTTTGGCTCTTCC
PRIMER_LEFT_13=814,20
PRIMER_RIGHT_13=1569,20
PRIMER_LEFT_13_TM=60.312
PRIMER_RIGHT_13_TM=59.077
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=756
PRIMER_PAIR_14_PENALTY=1.246241
PRIMER_LEFT_14_PENALTY=1.230989
PRIMER_RIGHT_14_PENALTY=0.015253
PRIMER_LEFT_14_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_14_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_14=853,20
PRIMER_RIGHT_14=1560,20
PRIMER_LEFT_14_TM=58.769
PRIMER_RIGHT_14_TM=60.015
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=708
PRIMER_PAIR_15_PENALTY=1.250755
PRIMER_LEFT_15_PENALTY=1.230989
PRIMER_RIGHT_15_PENALTY=0.019767
PRIMER_LEFT_15_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_15_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_15=853,20
PRIMER_RIGHT_15=1536,20
PRIMER_LEFT_15_TM=58.769
PRIMER_RIGHT_15_TM=60.020
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=684
PRIMER_PAIR_16_PENALTY=1.264669
PRIMER_LEFT_16_PENALTY=1.230989
PRIMER_RIGHT_16_PENALTY=0.033680
PRIMER_LEFT_16_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_16_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_16=853,20
PRIMER_RIGHT_16=1680,20
PRIMER_LEFT_16_TM=58.769
PRIMER_RIGHT_16_TM=59.966
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=828
PRIMER_PAIR_17_PENALTY=1.264669
PRIMER_LEFT_17_PENALTY=1.230989
PRIMER_RIGHT_17_PENALTY=0.033680
PRIMER_LEFT_17_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_17_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_17=853,20
PRIMER_RIGHT_17=1681,20
PRIMER_LEFT_17_TM=58.769
PRIMER_RIGHT_17_TM=59.966
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=829
PRIMER_PAIR_18_PENALTY=1.268471
PRIMER_LEFT_18_PENALTY=0.312496
PRIMER_RIGHT_18_PENALTY=0.955975
PRIMER_LEFT_18_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_18_SEQUENCE=GATGTTCAGCGTCTCTTGCC
PRIMER_LEFT_18=814,20
PRIMER_RIGHT_18=1537,20
PRIMER_LEFT_18_TM=60.312
PRIMER_RIGHT_18_TM=60.956
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=10.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=724
PRIMER_PAIR_19_PENALTY=1.338503
PRIMER_LEFT_19_PENALTY=0.312496
PRIMER_RIGHT_19_PENALTY=1.026007
PRIMER_LEFT_19_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_19_SEQUENCE=CCTAGCAGTTTGGCTCTTCCT
PRIMER_LEFT_19=814,20
PRIMER_RIGHT_19=1569,21
PRIMER_LEFT_19_TM=60.312
PRIMER_RIGHT_19_TM=60.026
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=756
PRIMER_PAIR_20_PENALTY=1.339574
PRIMER_LEFT_20_PENALTY=0.312496
PRIMER_RIGHT_20_PENALTY=1.027078
PRIMER_LEFT_20_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_20_SEQUENCE=TCGACTTAAAGCGGTTCCTT
PRIMER_LEFT_20=814,20
PRIMER_RIGHT_20=1633,20
PRIMER_LEFT_20_TM=60.312
PRIMER_RIGHT_20_TM=58.973
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=820
PRIMER_PAIR_21_PENALTY=1.345240
PRIMER_LEFT_21_PENALTY=0.312496
PRIMER_RIGHT_21_PENALTY=1.032744
PRIMER_LEFT_21_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_21_SEQUENCE=GGCTCTTCCTACGTTGCTCTT
PRIMER_LEFT_21=814,20
PRIMER_RIGHT_21=1558,21
PRIMER_LEFT_21_TM=60.312
PRIMER_RIGHT_21_TM=60.033
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=745
PRIMER_PAIR_22_PENALTY=1.385011
PRIMER_LEFT_22_PENALTY=1.230989
PRIMER_RIGHT_22_PENALTY=0.154023
PRIMER_LEFT_22_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_22_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_22=853,20
PRIMER_RIGHT_22=1571,20
PRIMER_LEFT_22_TM=58.769
PRIMER_RIGHT_22_TM=60.154
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=719
PRIMER_PAIR_23_PENALTY=1.412921
PRIMER_LEFT_23_PENALTY=0.312496
PRIMER_RIGHT_23_PENALTY=1.100425
PRIMER_LEFT_23_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_23_SEQUENCE=CTCGACTTAAAGCGGTTCCTT
PRIMER_LEFT_23=814,20
PRIMER_RIGHT_23=1634,21
PRIMER_LEFT_23_TM=60.312
PRIMER_RIGHT_23_TM=59.900
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=821
PRIMER_PAIR_24_PENALTY=1.469256
PRIMER_LEFT_24_PENALTY=0.312496
PRIMER_RIGHT_24_PENALTY=1.156760
PRIMER_LEFT_24_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_24_SEQUENCE=GCTCTTGATGTTCAGCGTCTC
PRIMER_LEFT_24=814,20
PRIMER_RIGHT_24=1543,21
PRIMER_LEFT_24_TM=60.312
PRIMER_RIGHT_24_TM=60.157
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=730
PRIMER_PAIR_25_PENALTY=1.497425
PRIMER_LEFT_25_PENALTY=1.230989
PRIMER_RIGHT_25_PENALTY=0.266437
PRIMER_LEFT_25_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_25_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_25=853,20
PRIMER_RIGHT_25=1329,20
PRIMER_LEFT_25_TM=58.769
PRIMER_RIGHT_25_TM=59.734
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=477
PRIMER_PAIR_26_PENALTY=1.542870
PRIMER_LEFT_26_PENALTY=0.312496
PRIMER_RIGHT_26_PENALTY=1.230374
PRIMER_LEFT_26_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_26_SEQUENCE=ATACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_26=814,20
PRIMER_RIGHT_26=1225,21
PRIMER_LEFT_26_TM=60.312
PRIMER_RIGHT_26_TM=60.230
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=6.3000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=412
PRIMER_PAIR_27_PENALTY=1.586561
PRIMER_LEFT_27_PENALTY=1.230989
PRIMER_RIGHT_27_PENALTY=0.355573
PRIMER_LEFT_27_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_27_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_27=853,20
PRIMER_RIGHT_27=1572,20
PRIMER_LEFT_27_TM=58.769
PRIMER_RIGHT_27_TM=59.644
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=720
PRIMER_PAIR_28_PENALTY=1.615228
PRIMER_LEFT_28_PENALTY=0.312496
PRIMER_RIGHT_28_PENALTY=1.302732
PRIMER_LEFT_28_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_28_SEQUENCE=GGCTCTTCCTACGTTGCTCT
PRIMER_LEFT_28=814,20
PRIMER_RIGHT_28=1558,20
PRIMER_LEFT_28_TM=60.312
PRIMER_RIGHT_28_TM=58.697
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=745
PRIMER_PAIR_29_PENALTY=1.634662
PRIMER_LEFT_29_PENALTY=1.230989
PRIMER_RIGHT_29_PENALTY=0.403673
PRIMER_LEFT_29_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_29_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_29=853,20
PRIMER_RIGHT_29=1545,20
PRIMER_LEFT_29_TM=58.769
PRIMER_RIGHT_29_TM=59.596
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=693
PRIMER_PAIR_30_PENALTY=1.644931
PRIMER_LEFT_30_PENALTY=1.629679
PRIMER_RIGHT_30_PENALTY=0.015253
PRIMER_LEFT_30_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_30_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_30=815,20
PRIMER_RIGHT_30=1560,20
PRIMER_LEFT_30_TM=58.370
PRIMER_RIGHT_30_TM=60.015
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=746
PRIMER_PAIR_31_PENALTY=1.649445
PRIMER_LEFT_31_PENALTY=1.629679
PRIMER_RIGHT_31_PENALTY=0.019767
PRIMER_LEFT_31_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_31_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_31=815,20
PRIMER_RIGHT_31=1536,20
PRIMER_LEFT_31_TM=58.370
PRIMER_RIGHT_31_TM=60.020
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=722
PRIMER_PAIR_32_PENALTY=1.663359
PRIMER_LEFT_32_PENALTY=1.629679
PRIMER_RIGHT_32_PENALTY=0.033680
PRIMER_LEFT_32_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_32_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_32=815,20
PRIMER_RIGHT_32=1680,20
PRIMER_LEFT_32_TM=58.370
PRIMER_RIGHT_32_TM=59.966
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=866
PRIMER_PAIR_33_PENALTY=1.663359
PRIMER_LEFT_33_PENALTY=1.629679
PRIMER_RIGHT_33_PENALTY=0.033680
PRIMER_LEFT_33_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_33_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_33=815,20
PRIMER_RIGHT_33=1681,20
PRIMER_LEFT_33_TM=58.370
PRIMER_RIGHT_33_TM=59.966
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=867
PRIMER_PAIR_34_PENALTY=1.689614
PRIMER_LEFT_34_PENALTY=0.312496
PRIMER_RIGHT_34_PENALTY=1.377118
PRIMER_LEFT_34_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_34_SEQUENCE=ATTGCACCAAACTTGTCCTG
PRIMER_LEFT_34=814,20
PRIMER_RIGHT_34=1333,20
PRIMER_LEFT_34_TM=60.312
PRIMER_RIGHT_34_TM=58.623
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=520
PRIMER_PAIR_35_PENALTY=1.689693
PRIMER_LEFT_35_PENALTY=1.674441
PRIMER_RIGHT_35_PENALTY=0.015253
PRIMER_LEFT_35_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_35_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_35=715,20
PRIMER_RIGHT_35=1560,20
PRIMER_LEFT_35_TM=58.326
PRIMER_RIGHT_35_TM=60.015
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=846
PRIMER_PAIR_36_PENALTY=1.691116
PRIMER_LEFT_36_PENALTY=0.312496
PRIMER_RIGHT_36_PENALTY=1.378620
PRIMER_LEFT_36_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_36_SEQUENCE=TCTCGACTTAAAGCGGTTCCT
PRIMER_LEFT_36=814,20
PRIMER_RIGHT_36=1635,21
PRIMER_LEFT_36_TM=60.312
PRIMER_RIGHT_36_TM=60.379
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=822
PRIMER_PAIR_37_PENALTY=1.694207
PRIMER_LEFT_37_PENALTY=1.674441
PRIMER_RIGHT_37_PENALTY=0.019767
PRIMER_LEFT_37_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_37_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_37=715,20
PRIMER_RIGHT_37=1536,20
PRIMER_LEFT_37_TM=58.326
PRIMER_RIGHT_37_TM=60.020
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=822
PRIMER_PAIR_38_PENALTY=1.699569
PRIMER_LEFT_38_PENALTY=1.674441
PRIMER_RIGHT_38_PENALTY=0.025128
PRIMER_LEFT_38_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_38_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_38=715,20
PRIMER_RIGHT_38=1224,20
PRIMER_LEFT_38_TM=58.326
PRIMER_RIGHT_38_TM=59.975
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=510
PRIMER_PAIR_39_PENALTY=1.708121
PRIMER_LEFT_39_PENALTY=1.674441
PRIMER_RIGHT_39_PENALTY=0.033680
PRIMER_LEFT_39_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_39_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_39=715,20
PRIMER_RIGHT_39=1680,20
PRIMER_LEFT_39_TM=58.326
PRIMER_RIGHT_39_TM=59.966
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=966
PRIMER_PAIR_40_PENALTY=1.708121
PRIMER_LEFT_40_PENALTY=1.674441
PRIMER_RIGHT_40_PENALTY=0.033680
PRIMER_LEFT_40_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_40_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_40=715,20
PRIMER_RIGHT_40=1681,20
PRIMER_LEFT_40_TM=58.326
PRIMER_RIGHT_40_TM=59.966
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=967
PRIMER_PAIR_41_PENALTY=1.710108
PRIMER_LEFT_41_PENALTY=0.312496
PRIMER_RIGHT_41_PENALTY=1.397612
PRIMER_LEFT_41_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_41_SEQUENCE=CTCGACTTAAAGCGGTTCCT
PRIMER_LEFT_41=814,20
PRIMER_RIGHT_41=1634,20
PRIMER_LEFT_41_TM=60.312
PRIMER_RIGHT_41_TM=58.602
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=821
PRIMER_PAIR_42_PENALTY=1.738510
PRIMER_LEFT_42_PENALTY=1.230989
PRIMER_RIGHT_42_PENALTY=0.507521
PRIMER_LEFT_42_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_42_SEQUENCE=CACGCCAAAGAAAGAGAGGA
PRIMER_LEFT_42=853,20
PRIMER_RIGHT_42=1828,20
PRIMER_LEFT_42_TM=58.769
PRIMER_RIGHT_42_TM=60.508
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=976
PRIMER_PAIR_43_PENALTY=1.738510
PRIMER_LEFT_43_PENALTY=1.230989
PRIMER_RIGHT_43_PENALTY=0.507521
PRIMER_LEFT_43_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_43_SEQUENCE=TCACGCCAAAGAAAGAGAGG
PRIMER_LEFT_43=853,20
PRIMER_RIGHT_43=1829,20
PRIMER_LEFT_43_TM=58.769
PRIMER_RIGHT_43_TM=60.508
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=977
PRIMER_PAIR_44_PENALTY=1.744180
PRIMER_LEFT_44_PENALTY=1.728928
PRIMER_RIGHT_44_PENALTY=0.015253
PRIMER_LEFT_44_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_44_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_44=815,21
PRIMER_RIGHT_44=1560,20
PRIMER_LEFT_44_TM=59.271
PRIMER_RIGHT_44_TM=60.015
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=746
PRIMER_PAIR_45_PENALTY=1.748694
PRIMER_LEFT_45_PENALTY=1.728928
PRIMER_RIGHT_45_PENALTY=0.019767
PRIMER_LEFT_45_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_45_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_45=815,21
PRIMER_RIGHT_45=1536,20
PRIMER_LEFT_45_TM=59.271
PRIMER_RIGHT_45_TM=60.020
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=722
PRIMER_PAIR_46_PENALTY=1.754056
PRIMER_LEFT_46_PENALTY=1.728928
PRIMER_RIGHT_46_PENALTY=0.025128
PRIMER_LEFT_46_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_46_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_46=815,21
PRIMER_RIGHT_46=1224,20
PRIMER_LEFT_46_TM=59.271
PRIMER_RIGHT_46_TM=59.975
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.3000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=410
PRIMER_PAIR_47_PENALTY=1.762608
PRIMER_LEFT_47_PENALTY=1.728928
PRIMER_RIGHT_47_PENALTY=0.033680
PRIMER_LEFT_47_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_47_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_47=815,21
PRIMER_RIGHT_47=1680,20
PRIMER_LEFT_47_TM=59.271
PRIMER_RIGHT_47_TM=59.966
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=866
PRIMER_PAIR_48_PENALTY=1.762608
PRIMER_LEFT_48_PENALTY=1.728928
PRIMER_RIGHT_48_PENALTY=0.033680
PRIMER_LEFT_48_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_48_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_48=815,21
PRIMER_RIGHT_48=1681,20
PRIMER_LEFT_48_TM=59.271
PRIMER_RIGHT_48_TM=59.966
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=867
PRIMER_PAIR_49_PENALTY=1.768043
PRIMER_LEFT_49_PENALTY=0.312496
PRIMER_RIGHT_49_PENALTY=1.455547
PRIMER_LEFT_49_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_49_SEQUENCE=GTTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_49=814,20
PRIMER_RIGHT_49=1546,21
PRIMER_LEFT_49_TM=60.312
PRIMER_RIGHT_49_TM=60.456
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=733
=
SEQUENCE_ID=MAGIC.105_4_15906294_15906386_po-0_can-0_512978_453196_TRUE
SEQUENCE_TARGET=950,192
SEQUENCE_TEMPLATE=CACTTGTTCCAATCATNACAGCCCTTGAATCNCCNAGATTTGCCACCATCACTTGATTTCCCTGTTCANNNNAATATTGTTGTTAANAATACACAATTTCNTAAAATGAACAATATTTTTGCAATGAAAAATAATAGATCCCCTAATATATAAAGCGGNANAAAACATTAAAGATATGAATAACTTACATGTTTAACGGCGAGAACAGCAGTAGTTCCAGAAGCAGAGCAATCATGAATCTTTTTAACTTTNAGAATNCTTTTGTCCATCTCCAAACANGATGTTTCGCANATGAGTTTCCAGTCTCGAGTCACTGAATGATTATTCATGTGGCCTAAGAGTATAGATGGCAATTGGTTCCTTACGTTCTTGCTCACAAACGCACCTCTCGGTCCATGCCCATCAAACACTCCACATAACGCTCCTTCTTCTGTTCCATANCCCTACAANTNTATCATTTCATTCCGTTAAAAACAAATTAATTGTTTTGAAGACAAACATATATATATTTTCAAAAGTATAAAGTACCAGGTGGAGAATGGCAGCATCTTGGTTCAAGCCCTTGCCTCCGGCTAAAGAAGAGACAGAGCCCAAGTTTTGAGGAACANCATGGTGGTCAGAGTATAAAACTGCATTTTCTTGTCCATAATCAGTTATCTCATAAATCTGNCTTTTATCTGTTGATCCTNCTGAGGATAAACAAAAACAAAATCCCNNNATTTTCTTTTCTTCTTTTGTGTTTTGCNNNNNNTTTNTTNTNTTTTTNGTTTGNTGTTTNNNNNATNTGTAAGAGGGAGATGATNNNACAAATGAAAAAGTTTGATACACGACTTNANTTATTGCATATNNNAGTTATATAGCCCTTTGTGAATCTTATTTAGGAATATATTAATACATACTTTTATTTTCATCTCGATGACTTGTATCTTTAGGCATTTAAAAAGAATNATTTTTAATTCNNNNNCGGTCGATCAATANTTNTNTTTTTCATAACATTTTTNNNAAAAATTGAATCCTTTTGATAANTAGAATANTTGATTATAAATGAGTCGTTTCCGCCAATTATATTAGAAATAAAGAATATGTAAAATGNGTCGACTAGTGAGAAACCGAATTATTTGACATACTCCAGTAAATTAAGAAATTANTATAGCTTATGCTGTAATGTAAGGCAATTATGTGGCTCCTGGTGGCAGTGACAACGTATAACAATTTACAGTTAATTAGTTAAAAATATNATCTGGANNNNNCCATCCCAATATTCATTTGAAAGGGCACATGACGTNGTGTGATTAGTATCTTGTTTAAACTACTAATTGATTTATTCCAGAATATAAATNAGAATTTNGAAGTTATCAATAGATTTTACCAACGGGATTNTTGTTCTTTGTCATTGCCAACAAGTTTAGACNCACCAAATATTTAATTATTTAAAGAAAAATTCATCCATNCGCTTGNTAACNNNNTATGTAANNNNAAAANNNNAAANGAAAGAGAACTGATGAAATGGAGATTAATCTAGCAGACTAGTAGTCAGGGTATGCGTTTGATCTTGAAAGTTTGAGTACCTTAAACCAAAAAANAANAAAAANCTTCTTTTNTTTTTGATTTNGTGTAGTGGTTTTATCGTTAATAGAGTTTNNTTTTGATCAATCTAATAACGTTTGATTCGGTTTTTAATCTGACAATAGACTATGTTGGGACACCAAGGTACATACANAGCACAAAACCATAGTTAATTTCCTCCAATAACCNTTTTTTTTCTATGTTAACTAACACTTACATAAAATTTACAGAAAATACTCCTCTTGCATTAATATAGTTTTACNAAAAATGNTTATGTAACACTTATTCTTATGTTGCAAAATGCTATGTAACNGTTATTGTTATGTTNCATATTAATGCAAGAGGAGTATACTTTTACAATTATTAAATAANNATTNTTTTTTCTAAANTTTTGTAAACGAAAANAAAAGAGTAATAATAAANAAATGTGACGTAAGTACTTTGTAATTACTAATTACTAATTAAGCCAGCTTTTCCTGACCTTTTGGACATATCAAACGATACAACAGGGTCAACTCTCACATCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.336529
PRIMER_LEFT_0_PENALTY=0.288804
PRIMER_RIGHT_0_PENALTY=1.047725
PRIMER_LEFT_0_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_0_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_0=587,20
PRIMER_RIGHT_0=1556,21
PRIMER_LEFT_0_TM=59.711
PRIMER_RIGHT_0_TM=59.952
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=970
PRIMER_PAIR_1_PENALTY=1.414176
PRIMER_LEFT_1_PENALTY=0.366450
PRIMER_RIGHT_1_PENALTY=1.047725
PRIMER_LEFT_1_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_1_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_1=584,20
PRIMER_RIGHT_1=1556,21
PRIMER_LEFT_1_TM=60.366
PRIMER_RIGHT_1_TM=59.952
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=1.705771
PRIMER_LEFT_2_PENALTY=0.288804
PRIMER_RIGHT_2_PENALTY=1.416967
PRIMER_LEFT_2_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_2_SEQUENCE=AAGATCAAACGCATACCCTGA
PRIMER_LEFT_2=587,20
PRIMER_RIGHT_2=1553,21
PRIMER_LEFT_2_TM=59.711
PRIMER_RIGHT_2_TM=59.583
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=967
PRIMER_PAIR_3_PENALTY=1.705771
PRIMER_LEFT_3_PENALTY=0.288804
PRIMER_RIGHT_3_PENALTY=1.416967
PRIMER_LEFT_3_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_3_SEQUENCE=TCAAGATCAAACGCATACCCT
PRIMER_LEFT_3=587,20
PRIMER_RIGHT_3=1555,21
PRIMER_LEFT_3_TM=59.711
PRIMER_RIGHT_3_TM=59.583
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=969
PRIMER_PAIR_4_PENALTY=1.715103
PRIMER_LEFT_4_PENALTY=0.288804
PRIMER_RIGHT_4_PENALTY=1.426299
PRIMER_LEFT_4_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_4_SEQUENCE=TCAAGATCAAACGCATACCC
PRIMER_LEFT_4=587,20
PRIMER_RIGHT_4=1555,20
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=58.574
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=969
PRIMER_PAIR_5_PENALTY=1.783418
PRIMER_LEFT_5_PENALTY=0.366450
PRIMER_RIGHT_5_PENALTY=1.416967
PRIMER_LEFT_5_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_5_SEQUENCE=AAGATCAAACGCATACCCTGA
PRIMER_LEFT_5=584,20
PRIMER_RIGHT_5=1553,21
PRIMER_LEFT_5_TM=60.366
PRIMER_RIGHT_5_TM=59.583
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=970
PRIMER_PAIR_6_PENALTY=1.783418
PRIMER_LEFT_6_PENALTY=0.366450
PRIMER_RIGHT_6_PENALTY=1.416967
PRIMER_LEFT_6_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_6_SEQUENCE=TCAAGATCAAACGCATACCCT
PRIMER_LEFT_6=584,20
PRIMER_RIGHT_6=1555,21
PRIMER_LEFT_6_TM=60.366
PRIMER_RIGHT_6_TM=59.583
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=9.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=972
PRIMER_PAIR_7_PENALTY=1.792749
PRIMER_LEFT_7_PENALTY=0.366450
PRIMER_RIGHT_7_PENALTY=1.426299
PRIMER_LEFT_7_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_7_SEQUENCE=TCAAGATCAAACGCATACCC
PRIMER_LEFT_7=584,20
PRIMER_RIGHT_7=1555,20
PRIMER_LEFT_7_TM=60.366
PRIMER_RIGHT_7_TM=58.574
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=972
PRIMER_PAIR_8_PENALTY=1.796616
PRIMER_LEFT_8_PENALTY=0.288804
PRIMER_RIGHT_8_PENALTY=1.507812
PRIMER_LEFT_8_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_8_SEQUENCE=CAAGATCAAACGCATACCCTG
PRIMER_LEFT_8=587,20
PRIMER_RIGHT_8=1554,21
PRIMER_LEFT_8_TM=59.711
PRIMER_RIGHT_8_TM=60.508
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=968
PRIMER_PAIR_9_PENALTY=1.874262
PRIMER_LEFT_9_PENALTY=0.366450
PRIMER_RIGHT_9_PENALTY=1.507812
PRIMER_LEFT_9_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_9_SEQUENCE=CAAGATCAAACGCATACCCTG
PRIMER_LEFT_9=584,20
PRIMER_RIGHT_9=1554,21
PRIMER_LEFT_9_TM=60.366
PRIMER_RIGHT_9_TM=60.508
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=971
PRIMER_PAIR_10_PENALTY=2.015641
PRIMER_LEFT_10_PENALTY=0.967916
PRIMER_RIGHT_10_PENALTY=1.047725
PRIMER_LEFT_10_SEQUENCE=GCCTCCGGCTAAAGAAGAGA
PRIMER_RIGHT_10_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_10=564,20
PRIMER_RIGHT_10=1556,21
PRIMER_LEFT_10_TM=60.968
PRIMER_RIGHT_10_TM=59.952
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=993
PRIMER_PAIR_11_PENALTY=2.115741
PRIMER_LEFT_11_PENALTY=0.288804
PRIMER_RIGHT_11_PENALTY=1.826937
PRIMER_LEFT_11_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_11_SEQUENCE=AGATCAAACGCATACCCTGA
PRIMER_LEFT_11=587,20
PRIMER_RIGHT_11=1552,20
PRIMER_LEFT_11_TM=59.711
PRIMER_RIGHT_11_TM=58.173
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=966
PRIMER_PAIR_12_PENALTY=2.138965
PRIMER_LEFT_12_PENALTY=1.091240
PRIMER_RIGHT_12_PENALTY=1.047725
PRIMER_LEFT_12_SEQUENCE=AGAGCCCAAGTTTTGAGGAA
PRIMER_RIGHT_12_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_12=585,20
PRIMER_RIGHT_12=1556,21
PRIMER_LEFT_12_TM=58.909
PRIMER_RIGHT_12_TM=59.952
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=972
PRIMER_PAIR_13_PENALTY=2.193388
PRIMER_LEFT_13_PENALTY=0.366450
PRIMER_RIGHT_13_PENALTY=1.826937
PRIMER_LEFT_13_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_13_SEQUENCE=AGATCAAACGCATACCCTGA
PRIMER_LEFT_13=584,20
PRIMER_RIGHT_13=1552,20
PRIMER_LEFT_13_TM=60.366
PRIMER_RIGHT_13_TM=58.173
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=969
PRIMER_PAIR_14_PENALTY=2.219312
PRIMER_LEFT_14_PENALTY=0.288804
PRIMER_RIGHT_14_PENALTY=1.930508
PRIMER_LEFT_14_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_14_SEQUENCE=GATCAAACGCATACCCTGACT
PRIMER_LEFT_14=587,20
PRIMER_RIGHT_14=1551,21
PRIMER_LEFT_14_TM=59.711
PRIMER_RIGHT_14_TM=59.069
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=965
PRIMER_PAIR_15_PENALTY=2.219312
PRIMER_LEFT_15_PENALTY=0.288804
PRIMER_RIGHT_15_PENALTY=1.930508
PRIMER_LEFT_15_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_15_SEQUENCE=AGATCAAACGCATACCCTGAC
PRIMER_LEFT_15=587,20
PRIMER_RIGHT_15=1552,21
PRIMER_LEFT_15_TM=59.711
PRIMER_RIGHT_15_TM=59.069
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=966
PRIMER_PAIR_16_PENALTY=2.270251
PRIMER_LEFT_16_PENALTY=0.288804
PRIMER_RIGHT_16_PENALTY=1.981447
PRIMER_LEFT_16_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_16_SEQUENCE=GATCAAACGCATACCCTGAC
PRIMER_LEFT_16=587,20
PRIMER_RIGHT_16=1551,20
PRIMER_LEFT_16_TM=59.711
PRIMER_RIGHT_16_TM=58.019
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=965
PRIMER_PAIR_17_PENALTY=2.281388
PRIMER_LEFT_17_PENALTY=1.233663
PRIMER_RIGHT_17_PENALTY=1.047725
PRIMER_LEFT_17_SEQUENCE=GAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_17_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_17=586,20
PRIMER_RIGHT_17=1556,21
PRIMER_LEFT_17_TM=58.766
PRIMER_RIGHT_17_TM=59.952
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=971
PRIMER_PAIR_18_PENALTY=2.296959
PRIMER_LEFT_18_PENALTY=0.366450
PRIMER_RIGHT_18_PENALTY=1.930508
PRIMER_LEFT_18_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_18_SEQUENCE=GATCAAACGCATACCCTGACT
PRIMER_LEFT_18=584,20
PRIMER_RIGHT_18=1551,21
PRIMER_LEFT_18_TM=60.366
PRIMER_RIGHT_18_TM=59.069
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=968
PRIMER_PAIR_19_PENALTY=2.296959
PRIMER_LEFT_19_PENALTY=0.366450
PRIMER_RIGHT_19_PENALTY=1.930508
PRIMER_LEFT_19_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_19_SEQUENCE=AGATCAAACGCATACCCTGAC
PRIMER_LEFT_19=584,20
PRIMER_RIGHT_19=1552,21
PRIMER_LEFT_19_TM=60.366
PRIMER_RIGHT_19_TM=59.069
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=969
PRIMER_PAIR_20_PENALTY=2.304637
PRIMER_LEFT_20_PENALTY=1.256912
PRIMER_RIGHT_20_PENALTY=1.047725
PRIMER_LEFT_20_SEQUENCE=AGAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_20_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_20=585,21
PRIMER_RIGHT_20=1556,21
PRIMER_LEFT_20_TM=59.743
PRIMER_RIGHT_20_TM=59.952
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=972
PRIMER_PAIR_21_PENALTY=2.314370
PRIMER_LEFT_21_PENALTY=0.288804
PRIMER_RIGHT_21_PENALTY=2.025566
PRIMER_LEFT_21_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_21_SEQUENCE=AGATCAAACGCATACCCTGACT
PRIMER_LEFT_21=587,20
PRIMER_RIGHT_21=1552,22
PRIMER_LEFT_21_TM=59.711
PRIMER_RIGHT_21_TM=60.026
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=966
PRIMER_PAIR_22_PENALTY=2.347897
PRIMER_LEFT_22_PENALTY=0.366450
PRIMER_RIGHT_22_PENALTY=1.981447
PRIMER_LEFT_22_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_22_SEQUENCE=GATCAAACGCATACCCTGAC
PRIMER_LEFT_22=584,20
PRIMER_RIGHT_22=1551,20
PRIMER_LEFT_22_TM=60.366
PRIMER_RIGHT_22_TM=58.019
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=968
PRIMER_PAIR_23_PENALTY=2.384883
PRIMER_LEFT_23_PENALTY=0.967916
PRIMER_RIGHT_23_PENALTY=1.416967
PRIMER_LEFT_23_SEQUENCE=GCCTCCGGCTAAAGAAGAGA
PRIMER_RIGHT_23_SEQUENCE=AAGATCAAACGCATACCCTGA
PRIMER_LEFT_23=564,20
PRIMER_RIGHT_23=1553,21
PRIMER_LEFT_23_TM=60.968
PRIMER_RIGHT_23_TM=59.583
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=990
PRIMER_PAIR_24_PENALTY=2.384883
PRIMER_LEFT_24_PENALTY=0.967916
PRIMER_RIGHT_24_PENALTY=1.416967
PRIMER_LEFT_24_SEQUENCE=GCCTCCGGCTAAAGAAGAGA
PRIMER_RIGHT_24_SEQUENCE=TCAAGATCAAACGCATACCCT
PRIMER_LEFT_24=564,20
PRIMER_RIGHT_24=1555,21
PRIMER_LEFT_24_TM=60.968
PRIMER_RIGHT_24_TM=59.583
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=9.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=992
PRIMER_PAIR_25_PENALTY=2.392016
PRIMER_LEFT_25_PENALTY=0.366450
PRIMER_RIGHT_25_PENALTY=2.025566
PRIMER_LEFT_25_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_25_SEQUENCE=AGATCAAACGCATACCCTGACT
PRIMER_LEFT_25=584,20
PRIMER_RIGHT_25=1552,22
PRIMER_LEFT_25_TM=60.366
PRIMER_RIGHT_25_TM=60.026
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=969
PRIMER_PAIR_26_PENALTY=2.394215
PRIMER_LEFT_26_PENALTY=0.967916
PRIMER_RIGHT_26_PENALTY=1.426299
PRIMER_LEFT_26_SEQUENCE=GCCTCCGGCTAAAGAAGAGA
PRIMER_RIGHT_26_SEQUENCE=TCAAGATCAAACGCATACCC
PRIMER_LEFT_26=564,20
PRIMER_RIGHT_26=1555,20
PRIMER_LEFT_26_TM=60.968
PRIMER_RIGHT_26_TM=58.574
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=992
PRIMER_PAIR_27_PENALTY=2.399543
PRIMER_LEFT_27_PENALTY=1.351817
PRIMER_RIGHT_27_PENALTY=1.047725
PRIMER_LEFT_27_SEQUENCE=CTTGCCTCCGGCTAAAGAAG
PRIMER_RIGHT_27_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_27=561,20
PRIMER_RIGHT_27=1556,21
PRIMER_LEFT_27_TM=61.352
PRIMER_RIGHT_27_TM=59.952
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=8.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=996
PRIMER_PAIR_28_PENALTY=2.415667
PRIMER_LEFT_28_PENALTY=0.010545
PRIMER_RIGHT_28_PENALTY=2.405122
PRIMER_LEFT_28_SEQUENCE=ACGCTCCTTCTTCTGTTCCA
PRIMER_RIGHT_28_SEQUENCE=GGAGCCACATAATTGCCTTAC
PRIMER_LEFT_28=418,20
PRIMER_RIGHT_28=1186,21
PRIMER_LEFT_28_TM=59.989
PRIMER_RIGHT_28_TM=58.595
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=5.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=769
PRIMER_PAIR_29_PENALTY=2.475728
PRIMER_LEFT_29_PENALTY=0.967916
PRIMER_RIGHT_29_PENALTY=1.507812
PRIMER_LEFT_29_SEQUENCE=GCCTCCGGCTAAAGAAGAGA
PRIMER_RIGHT_29_SEQUENCE=CAAGATCAAACGCATACCCTG
PRIMER_LEFT_29=564,20
PRIMER_RIGHT_29=1554,21
PRIMER_LEFT_29_TM=60.968
PRIMER_RIGHT_29_TM=60.508
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=991
PRIMER_PAIR_30_PENALTY=2.482902
PRIMER_LEFT_30_PENALTY=0.010545
PRIMER_RIGHT_30_PENALTY=2.472356
PRIMER_LEFT_30_SEQUENCE=ACGCTCCTTCTTCTGTTCCA
PRIMER_RIGHT_30_SEQUENCE=AGGAGCCACATAATTGCCTTAC
PRIMER_LEFT_30=418,20
PRIMER_RIGHT_30=1187,22
PRIMER_LEFT_30_TM=59.989
PRIMER_RIGHT_30_TM=59.528
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=5.7000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=770
PRIMER_PAIR_31_PENALTY=2.508207
PRIMER_LEFT_31_PENALTY=1.091240
PRIMER_RIGHT_31_PENALTY=1.416967
PRIMER_LEFT_31_SEQUENCE=AGAGCCCAAGTTTTGAGGAA
PRIMER_RIGHT_31_SEQUENCE=AAGATCAAACGCATACCCTGA
PRIMER_LEFT_31=585,20
PRIMER_RIGHT_31=1553,21
PRIMER_LEFT_31_TM=58.909
PRIMER_RIGHT_31_TM=59.583
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=969
PRIMER_PAIR_32_PENALTY=2.508207
PRIMER_LEFT_32_PENALTY=1.091240
PRIMER_RIGHT_32_PENALTY=1.416967
PRIMER_LEFT_32_SEQUENCE=AGAGCCCAAGTTTTGAGGAA
PRIMER_RIGHT_32_SEQUENCE=TCAAGATCAAACGCATACCCT
PRIMER_LEFT_32=585,20
PRIMER_RIGHT_32=1555,21
PRIMER_LEFT_32_TM=58.909
PRIMER_RIGHT_32_TM=59.583
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.1000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=971
PRIMER_PAIR_33_PENALTY=2.517538
PRIMER_LEFT_33_PENALTY=1.091240
PRIMER_RIGHT_33_PENALTY=1.426299
PRIMER_LEFT_33_SEQUENCE=AGAGCCCAAGTTTTGAGGAA
PRIMER_RIGHT_33_SEQUENCE=TCAAGATCAAACGCATACCC
PRIMER_LEFT_33=585,20
PRIMER_RIGHT_33=1555,20
PRIMER_LEFT_33_TM=58.909
PRIMER_RIGHT_33_TM=58.574
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=971
PRIMER_PAIR_34_PENALTY=2.584631
PRIMER_LEFT_34_PENALTY=1.536905
PRIMER_RIGHT_34_PENALTY=1.047725
PRIMER_LEFT_34_SEQUENCE=GAGACAGAGCCCAAGTTTTGA
PRIMER_RIGHT_34_SEQUENCE=TTCAAGATCAAACGCATACCC
PRIMER_LEFT_34=580,21
PRIMER_RIGHT_34=1556,21
PRIMER_LEFT_34_TM=59.463
PRIMER_RIGHT_34_TM=59.952
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.3000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=977
PRIMER_PAIR_35_PENALTY=2.590427
PRIMER_LEFT_35_PENALTY=0.288804
PRIMER_RIGHT_35_PENALTY=2.301623
PRIMER_LEFT_35_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_35_SEQUENCE=AAGATCAAACGCATACCCTG
PRIMER_LEFT_35=587,20
PRIMER_RIGHT_35=1553,20
PRIMER_LEFT_35_TM=59.711
PRIMER_RIGHT_35_TM=57.698
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=967
PRIMER_PAIR_36_PENALTY=2.590427
PRIMER_LEFT_36_PENALTY=0.288804
PRIMER_RIGHT_36_PENALTY=2.301623
PRIMER_LEFT_36_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_36_SEQUENCE=CAAGATCAAACGCATACCCT
PRIMER_LEFT_36=587,20
PRIMER_RIGHT_36=1554,20
PRIMER_LEFT_36_TM=59.711
PRIMER_RIGHT_36_TM=57.698
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=9.1000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=968
PRIMER_PAIR_37_PENALTY=2.599052
PRIMER_LEFT_37_PENALTY=1.091240
PRIMER_RIGHT_37_PENALTY=1.507812
PRIMER_LEFT_37_SEQUENCE=AGAGCCCAAGTTTTGAGGAA
PRIMER_RIGHT_37_SEQUENCE=CAAGATCAAACGCATACCCTG
PRIMER_LEFT_37=585,20
PRIMER_RIGHT_37=1554,21
PRIMER_LEFT_37_TM=58.909
PRIMER_RIGHT_37_TM=60.508
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=970
PRIMER_PAIR_38_PENALTY=2.631265
PRIMER_LEFT_38_PENALTY=0.226143
PRIMER_RIGHT_38_PENALTY=2.405122
PRIMER_LEFT_38_SEQUENCE=GAGAATGGCAGCATCTTGGT
PRIMER_RIGHT_38_SEQUENCE=GGAGCCACATAATTGCCTTAC
PRIMER_LEFT_38=534,20
PRIMER_RIGHT_38=1186,21
PRIMER_LEFT_38_TM=60.226
PRIMER_RIGHT_38_TM=58.595
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=5.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=653
PRIMER_PAIR_39_PENALTY=2.631265
PRIMER_LEFT_39_PENALTY=0.226143
PRIMER_RIGHT_39_PENALTY=2.405122
PRIMER_LEFT_39_SEQUENCE=GTGGAGAATGGCAGCATCTT
PRIMER_RIGHT_39_SEQUENCE=GGAGCCACATAATTGCCTTAC
PRIMER_LEFT_39=531,20
PRIMER_RIGHT_39=1186,21
PRIMER_LEFT_39_TM=60.226
PRIMER_RIGHT_39_TM=58.595
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=5.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=656
PRIMER_PAIR_40_PENALTY=2.650630
PRIMER_LEFT_40_PENALTY=1.233663
PRIMER_RIGHT_40_PENALTY=1.416967
PRIMER_LEFT_40_SEQUENCE=GAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_40_SEQUENCE=AAGATCAAACGCATACCCTGA
PRIMER_LEFT_40=586,20
PRIMER_RIGHT_40=1553,21
PRIMER_LEFT_40_TM=58.766
PRIMER_RIGHT_40_TM=59.583
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=968
PRIMER_PAIR_41_PENALTY=2.650630
PRIMER_LEFT_41_PENALTY=1.233663
PRIMER_RIGHT_41_PENALTY=1.416967
PRIMER_LEFT_41_SEQUENCE=GAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_41_SEQUENCE=TCAAGATCAAACGCATACCCT
PRIMER_LEFT_41=586,20
PRIMER_RIGHT_41=1555,21
PRIMER_LEFT_41_TM=58.766
PRIMER_RIGHT_41_TM=59.583
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=9.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=970
PRIMER_PAIR_42_PENALTY=2.659961
PRIMER_LEFT_42_PENALTY=1.233663
PRIMER_RIGHT_42_PENALTY=1.426299
PRIMER_LEFT_42_SEQUENCE=GAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_42_SEQUENCE=TCAAGATCAAACGCATACCC
PRIMER_LEFT_42=586,20
PRIMER_RIGHT_42=1555,20
PRIMER_LEFT_42_TM=58.766
PRIMER_RIGHT_42_TM=58.574
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=970
PRIMER_PAIR_43_PENALTY=2.668074
PRIMER_LEFT_43_PENALTY=0.366450
PRIMER_RIGHT_43_PENALTY=2.301623
PRIMER_LEFT_43_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_43_SEQUENCE=AAGATCAAACGCATACCCTG
PRIMER_LEFT_43=584,20
PRIMER_RIGHT_43=1553,20
PRIMER_LEFT_43_TM=60.366
PRIMER_RIGHT_43_TM=57.698
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=970
PRIMER_PAIR_44_PENALTY=2.668074
PRIMER_LEFT_44_PENALTY=0.366450
PRIMER_RIGHT_44_PENALTY=2.301623
PRIMER_LEFT_44_SEQUENCE=CAGAGCCCAAGTTTTGAGGA
PRIMER_RIGHT_44_SEQUENCE=CAAGATCAAACGCATACCCT
PRIMER_LEFT_44=584,20
PRIMER_RIGHT_44=1554,20
PRIMER_LEFT_44_TM=60.366
PRIMER_RIGHT_44_TM=57.698
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=9.1000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=971
PRIMER_PAIR_45_PENALTY=2.669341
PRIMER_LEFT_45_PENALTY=0.288804
PRIMER_RIGHT_45_PENALTY=2.380537
PRIMER_LEFT_45_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_45_SEQUENCE=AAGATCAAACGCATACCCTGAC
PRIMER_LEFT_45=587,20
PRIMER_RIGHT_45=1553,22
PRIMER_LEFT_45_TM=59.711
PRIMER_RIGHT_45_TM=60.381
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=967
PRIMER_PAIR_46_PENALTY=2.673879
PRIMER_LEFT_46_PENALTY=1.256912
PRIMER_RIGHT_46_PENALTY=1.416967
PRIMER_LEFT_46_SEQUENCE=AGAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_46_SEQUENCE=AAGATCAAACGCATACCCTGA
PRIMER_LEFT_46=585,21
PRIMER_RIGHT_46=1553,21
PRIMER_LEFT_46_TM=59.743
PRIMER_RIGHT_46_TM=59.583
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=969
PRIMER_PAIR_47_PENALTY=2.673879
PRIMER_LEFT_47_PENALTY=1.256912
PRIMER_RIGHT_47_PENALTY=1.416967
PRIMER_LEFT_47_SEQUENCE=AGAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_47_SEQUENCE=TCAAGATCAAACGCATACCCT
PRIMER_LEFT_47=585,21
PRIMER_RIGHT_47=1555,21
PRIMER_LEFT_47_TM=59.743
PRIMER_RIGHT_47_TM=59.583
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=9.1000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=971
PRIMER_PAIR_48_PENALTY=2.683211
PRIMER_LEFT_48_PENALTY=1.256912
PRIMER_RIGHT_48_PENALTY=1.426299
PRIMER_LEFT_48_SEQUENCE=AGAGCCCAAGTTTTGAGGAAC
PRIMER_RIGHT_48_SEQUENCE=TCAAGATCAAACGCATACCC
PRIMER_LEFT_48=585,21
PRIMER_RIGHT_48=1555,20
PRIMER_LEFT_48_TM=59.743
PRIMER_RIGHT_48_TM=58.574
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=971
PRIMER_PAIR_49_PENALTY=2.693926
PRIMER_LEFT_49_PENALTY=0.288804
PRIMER_RIGHT_49_PENALTY=2.405122
PRIMER_LEFT_49_SEQUENCE=AGCCCAAGTTTTGAGGAACA
PRIMER_RIGHT_49_SEQUENCE=GGAGCCACATAATTGCCTTAC
PRIMER_LEFT_49=587,20
PRIMER_RIGHT_49=1186,21
PRIMER_LEFT_49_TM=59.711
PRIMER_RIGHT_49_TM=58.595
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=5.7000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=600
=
SEQUENCE_ID=MAGIC.105_3_14522522_14522698_can-0_sf-2_43862_14099_TRUE
SEQUENCE_TARGET=950,276
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_5_12993427_12993489_wil-2_zu-0_218475_55380_TRUE
SEQUENCE_TARGET=950,162
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_15115470_15115505_po-0_rsch-4_9810_104104_TRUE
SEQUENCE_TARGET=950,135
SEQUENCE_TEMPLATE=ACNNAATAAGCAAAATATNNNNNNTNTNNNTCTAAATTCATCAGTTGAATTACTTTTGGTNTTTNTNNNNNNNNNNTAANAAAATAAGAATACTTTTGAANNCTAACNTNCNTGNNNNNNNNNNNNAAACATCTCTCAGATCTANACAACATCTACAAACTNNNNNNNNTTAAAANGNNNNANAANTTNNNAGTAAANAANACAATAATANACCTTGTGTCTNNTNATNTAGATCTTCTCAATCAATNATAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCAGTTGACANGAGTTNNATANANNACANANNAAAANATNTATTTTAAATANGAGGAAGAGAAAATTAAATATTNACCTTTTGGAGGACAACTACANCATATANGTTTTGGTGAAGGCTAGAAAAATAATAGCGGCTCCTGCACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTCATTNATATTNATTCTTGATTCCNTCATATTGTGTGTCATTAGCTAGGAAAAACGCCACTATTTATAANNTATATGAGTTAAGTAGTAGGGTTTAANAGNNNNATCATGACTTTTNCATTTAATCCATACNNTGTAGNTCTAATACTGACTTATNAAAANNANACGTTAGTAGATTAATTGGAGATTAGTGAAACTATANGTTACGAAANNNNNGTTGTGTATTTATTGNTTTNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGGCCGTGAGGACAAAGAAATCGGAAATGGAGGAAACATCGGAGAGAAGGAGAAGGGTAGTTTNGGTTCCAGTTCCAGCTCAAGGACACATATCTCCGATGATGCAACTTGGGAGAGCCCNTTCTTTAAAGGGTTTCTCGATTACAGTTGCTCAGACTAAGTTCAATTACTTAAACCCTTCCAAAGACTTAGCTGATTTTCAGTTCATCACCATCCCAGAGAGCTTACCAGCCTCGGATCTGAAAACTCTTGGGCCAATTTGGTTTATTATTAAACTCAACAAAGAGTGTGAAATTAGCTTCAAGAAGTGTTTAGGTCAGTTTTTGCTGCAACAACAGGAAGAGATCGCATGTGTCATCTACGACGAGTTCATGTACTTTGCTGAAGCTGCAGCCAANGAGTTTAACCTTCCCAAAGTTATTTTCAGTACCGAAAATGCGACGGCTTTTGCTTGTCGCTCTGCCATGTGCAAACTCTATGCAAAAGATGGTATAGCTCCCCTTANAGGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.735679
PRIMER_LEFT_0_PENALTY=1.606344
PRIMER_RIGHT_0_PENALTY=0.129335
PRIMER_LEFT_0_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_0_SEQUENCE=TCCGATGTTTCCTCCATTTC
PRIMER_LEFT_0=633,21
PRIMER_RIGHT_0=1568,20
PRIMER_LEFT_0_TM=59.394
PRIMER_RIGHT_0_TM=59.871
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=11.7000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=936
PRIMER_PAIR_1_PENALTY=1.762404
PRIMER_LEFT_1_PENALTY=1.606344
PRIMER_RIGHT_1_PENALTY=0.156061
PRIMER_LEFT_1_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_1_SEQUENCE=CCTTGAGCTGGAACTGGAAC
PRIMER_LEFT_1=633,21
PRIMER_RIGHT_1=1610,20
PRIMER_LEFT_1_TM=59.394
PRIMER_RIGHT_1_TM=59.844
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=978
PRIMER_PAIR_2_PENALTY=1.992100
PRIMER_LEFT_2_PENALTY=1.606344
PRIMER_RIGHT_2_PENALTY=0.385756
PRIMER_LEFT_2_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_2_SEQUENCE=GTCCTTGAGCTGGAACTGGA
PRIMER_LEFT_2=633,21
PRIMER_RIGHT_2=1612,20
PRIMER_LEFT_2_TM=59.394
PRIMER_RIGHT_2_TM=60.386
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=980
PRIMER_PAIR_3_PENALTY=2.123019
PRIMER_LEFT_3_PENALTY=1.606344
PRIMER_RIGHT_3_PENALTY=0.516675
PRIMER_LEFT_3_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_3_SEQUENCE=TCTCCGATGTTTCCTCCATT
PRIMER_LEFT_3=633,21
PRIMER_RIGHT_3=1570,20
PRIMER_LEFT_3_TM=59.394
PRIMER_RIGHT_3_TM=59.483
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=938
PRIMER_PAIR_4_PENALTY=2.232759
PRIMER_LEFT_4_PENALTY=1.606344
PRIMER_RIGHT_4_PENALTY=0.626415
PRIMER_LEFT_4_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_4_SEQUENCE=CGATTTCTTTGTCCTCACGG
PRIMER_LEFT_4=633,21
PRIMER_RIGHT_4=1548,20
PRIMER_LEFT_4_TM=59.394
PRIMER_RIGHT_4_TM=60.626
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=916
PRIMER_PAIR_5_PENALTY=2.243305
PRIMER_LEFT_5_PENALTY=1.606344
PRIMER_RIGHT_5_PENALTY=0.636961
PRIMER_LEFT_5_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_5_SEQUENCE=TGTTTCCTCCATTTCCGATT
PRIMER_LEFT_5=633,21
PRIMER_RIGHT_5=1563,20
PRIMER_LEFT_5_TM=59.394
PRIMER_RIGHT_5_TM=59.363
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=11.7000
PRIMER_RIGHT_5_END_STABILITY=8.6000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=931
PRIMER_PAIR_6_PENALTY=2.276718
PRIMER_LEFT_6_PENALTY=2.147383
PRIMER_RIGHT_6_PENALTY=0.129335
PRIMER_LEFT_6_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_6_SEQUENCE=TCCGATGTTTCCTCCATTTC
PRIMER_LEFT_6=633,22
PRIMER_RIGHT_6=1568,20
PRIMER_LEFT_6_TM=60.147
PRIMER_RIGHT_6_TM=59.871
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=936
PRIMER_PAIR_7_PENALTY=2.303444
PRIMER_LEFT_7_PENALTY=2.147383
PRIMER_RIGHT_7_PENALTY=0.156061
PRIMER_LEFT_7_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_7_SEQUENCE=CCTTGAGCTGGAACTGGAAC
PRIMER_LEFT_7=633,22
PRIMER_RIGHT_7=1610,20
PRIMER_LEFT_7_TM=60.147
PRIMER_RIGHT_7_TM=59.844
PRIMER_LEFT_7_GC_PERCENT=45.455
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=978
PRIMER_PAIR_8_PENALTY=2.342903
PRIMER_LEFT_8_PENALTY=1.606344
PRIMER_RIGHT_8_PENALTY=0.736559
PRIMER_LEFT_8_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_8_SEQUENCE=TCTCTCCGATGTTTCCTCCA
PRIMER_LEFT_8=633,21
PRIMER_RIGHT_8=1572,20
PRIMER_LEFT_8_TM=59.394
PRIMER_RIGHT_8_TM=60.737
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=11.7000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=940
PRIMER_PAIR_9_PENALTY=2.392850
PRIMER_LEFT_9_PENALTY=1.606344
PRIMER_RIGHT_9_PENALTY=0.786506
PRIMER_LEFT_9_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_9_SEQUENCE=TTCTCTCCGATGTTTCCTCC
PRIMER_LEFT_9=633,21
PRIMER_RIGHT_9=1573,20
PRIMER_LEFT_9_TM=59.394
PRIMER_RIGHT_9_TM=59.213
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=11.7000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=941
PRIMER_PAIR_10_PENALTY=2.392850
PRIMER_LEFT_10_PENALTY=1.606344
PRIMER_RIGHT_10_PENALTY=0.786506
PRIMER_LEFT_10_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_10_SEQUENCE=TCCTTCTCTCCGATGTTTCC
PRIMER_LEFT_10=633,21
PRIMER_RIGHT_10=1576,20
PRIMER_LEFT_10_TM=59.394
PRIMER_RIGHT_10_TM=59.213
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.7000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=944
PRIMER_PAIR_11_PENALTY=2.519044
PRIMER_LEFT_11_PENALTY=1.606344
PRIMER_RIGHT_11_PENALTY=0.912701
PRIMER_LEFT_11_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_11_SEQUENCE=TCCTTGAGCTGGAACTGGAA
PRIMER_LEFT_11=633,21
PRIMER_RIGHT_11=1611,20
PRIMER_LEFT_11_TM=59.394
PRIMER_RIGHT_11_TM=60.913
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=11.7000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=979
PRIMER_PAIR_12_PENALTY=2.533139
PRIMER_LEFT_12_PENALTY=2.147383
PRIMER_RIGHT_12_PENALTY=0.385756
PRIMER_LEFT_12_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_12_SEQUENCE=GTCCTTGAGCTGGAACTGGA
PRIMER_LEFT_12=633,22
PRIMER_RIGHT_12=1612,20
PRIMER_LEFT_12_TM=60.147
PRIMER_RIGHT_12_TM=60.386
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=980
PRIMER_PAIR_13_PENALTY=2.597430
PRIMER_LEFT_13_PENALTY=2.468095
PRIMER_RIGHT_13_PENALTY=0.129335
PRIMER_LEFT_13_SEQUENCE=TCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_13_SEQUENCE=TCCGATGTTTCCTCCATTTC
PRIMER_LEFT_13=632,21
PRIMER_RIGHT_13=1568,20
PRIMER_LEFT_13_TM=58.532
PRIMER_RIGHT_13_TM=59.871
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.1000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=937
PRIMER_PAIR_14_PENALTY=2.624155
PRIMER_LEFT_14_PENALTY=2.468095
PRIMER_RIGHT_14_PENALTY=0.156061
PRIMER_LEFT_14_SEQUENCE=TCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_14_SEQUENCE=CCTTGAGCTGGAACTGGAAC
PRIMER_LEFT_14=632,21
PRIMER_RIGHT_14=1610,20
PRIMER_LEFT_14_TM=58.532
PRIMER_RIGHT_14_TM=59.844
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.1000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=979
PRIMER_PAIR_15_PENALTY=2.625056
PRIMER_LEFT_15_PENALTY=1.606344
PRIMER_RIGHT_15_PENALTY=1.018712
PRIMER_LEFT_15_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_15_SEQUENCE=CTCCGATGTTTCCTCCATTT
PRIMER_LEFT_15=633,21
PRIMER_RIGHT_15=1569,20
PRIMER_LEFT_15_TM=59.394
PRIMER_RIGHT_15_TM=58.981
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.7000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=937
PRIMER_PAIR_16_PENALTY=2.643082
PRIMER_LEFT_16_PENALTY=1.606344
PRIMER_RIGHT_16_PENALTY=1.036738
PRIMER_LEFT_16_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_16_SEQUENCE=CCTTCTCCTTCTCTCCGATG
PRIMER_LEFT_16=633,21
PRIMER_RIGHT_16=1581,20
PRIMER_LEFT_16_TM=59.394
PRIMER_RIGHT_16_TM=58.963
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=11.7000
PRIMER_RIGHT_16_END_STABILITY=8.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=949
PRIMER_PAIR_17_PENALTY=2.664058
PRIMER_LEFT_17_PENALTY=2.147383
PRIMER_RIGHT_17_PENALTY=0.516675
PRIMER_LEFT_17_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_17_SEQUENCE=TCTCCGATGTTTCCTCCATT
PRIMER_LEFT_17=633,22
PRIMER_RIGHT_17=1570,20
PRIMER_LEFT_17_TM=60.147
PRIMER_RIGHT_17_TM=59.483
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=938
PRIMER_PAIR_18_PENALTY=2.773798
PRIMER_LEFT_18_PENALTY=2.147383
PRIMER_RIGHT_18_PENALTY=0.626415
PRIMER_LEFT_18_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_18_SEQUENCE=CGATTTCTTTGTCCTCACGG
PRIMER_LEFT_18=633,22
PRIMER_RIGHT_18=1548,20
PRIMER_LEFT_18_TM=60.147
PRIMER_RIGHT_18_TM=60.626
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=916
PRIMER_PAIR_19_PENALTY=2.780853
PRIMER_LEFT_19_PENALTY=1.606344
PRIMER_RIGHT_19_PENALTY=1.174509
PRIMER_LEFT_19_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_19_SEQUENCE=CCTTCTCCTTCTCTCCGATGT
PRIMER_LEFT_19=633,21
PRIMER_RIGHT_19=1581,21
PRIMER_LEFT_19_TM=59.394
PRIMER_RIGHT_19_TM=59.825
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=11.7000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=949
PRIMER_PAIR_20_PENALTY=2.784344
PRIMER_LEFT_20_PENALTY=2.147383
PRIMER_RIGHT_20_PENALTY=0.636961
PRIMER_LEFT_20_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_20_SEQUENCE=TGTTTCCTCCATTTCCGATT
PRIMER_LEFT_20=633,22
PRIMER_RIGHT_20=1563,20
PRIMER_LEFT_20_TM=60.147
PRIMER_RIGHT_20_TM=59.363
PRIMER_LEFT_20_GC_PERCENT=45.455
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=8.6000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=931
PRIMER_PAIR_21_PENALTY=2.803566
PRIMER_LEFT_21_PENALTY=1.606344
PRIMER_RIGHT_21_PENALTY=1.197222
PRIMER_LEFT_21_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_21_SEQUENCE=CTTCTCTCCGATGTTTCCTCC
PRIMER_LEFT_21=633,21
PRIMER_RIGHT_21=1574,21
PRIMER_LEFT_21_TM=59.394
PRIMER_RIGHT_21_TM=60.197
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=11.7000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=942
PRIMER_PAIR_22_PENALTY=2.803566
PRIMER_LEFT_22_PENALTY=1.606344
PRIMER_RIGHT_22_PENALTY=1.197222
PRIMER_LEFT_22_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_22_SEQUENCE=CCTTCTCTCCGATGTTTCCTC
PRIMER_LEFT_22=633,21
PRIMER_RIGHT_22=1575,21
PRIMER_LEFT_22_TM=59.394
PRIMER_RIGHT_22_TM=60.197
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=11.7000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=943
PRIMER_PAIR_23_PENALTY=2.803566
PRIMER_LEFT_23_PENALTY=1.606344
PRIMER_RIGHT_23_PENALTY=1.197222
PRIMER_LEFT_23_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_23_SEQUENCE=CTCCTTCTCTCCGATGTTTCC
PRIMER_LEFT_23=633,21
PRIMER_RIGHT_23=1577,21
PRIMER_LEFT_23_TM=59.394
PRIMER_RIGHT_23_TM=60.197
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=11.7000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=945
PRIMER_PAIR_24_PENALTY=2.814556
PRIMER_LEFT_24_PENALTY=2.685220
PRIMER_RIGHT_24_PENALTY=0.129335
PRIMER_LEFT_24_SEQUENCE=GTCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_24_SEQUENCE=TCCGATGTTTCCTCCATTTC
PRIMER_LEFT_24=631,22
PRIMER_RIGHT_24=1568,20
PRIMER_LEFT_24_TM=59.315
PRIMER_RIGHT_24_TM=59.871
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=11.1000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=938
PRIMER_PAIR_25_PENALTY=2.841281
PRIMER_LEFT_25_PENALTY=2.685220
PRIMER_RIGHT_25_PENALTY=0.156061
PRIMER_LEFT_25_SEQUENCE=GTCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_25_SEQUENCE=CCTTGAGCTGGAACTGGAAC
PRIMER_LEFT_25=631,22
PRIMER_RIGHT_25=1610,20
PRIMER_LEFT_25_TM=59.315
PRIMER_RIGHT_25_TM=59.844
PRIMER_LEFT_25_GC_PERCENT=45.455
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=11.1000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=980
PRIMER_PAIR_26_PENALTY=2.853851
PRIMER_LEFT_26_PENALTY=2.468095
PRIMER_RIGHT_26_PENALTY=0.385756
PRIMER_LEFT_26_SEQUENCE=TCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_26_SEQUENCE=GTCCTTGAGCTGGAACTGGA
PRIMER_LEFT_26=632,21
PRIMER_RIGHT_26=1612,20
PRIMER_LEFT_26_TM=58.532
PRIMER_RIGHT_26_TM=60.386
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=11.1000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=981
PRIMER_PAIR_27_PENALTY=2.883942
PRIMER_LEFT_27_PENALTY=2.147383
PRIMER_RIGHT_27_PENALTY=0.736559
PRIMER_LEFT_27_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_27_SEQUENCE=TCTCTCCGATGTTTCCTCCA
PRIMER_LEFT_27=633,22
PRIMER_RIGHT_27=1572,20
PRIMER_LEFT_27_TM=60.147
PRIMER_RIGHT_27_TM=60.737
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=940
PRIMER_PAIR_28_PENALTY=2.903898
PRIMER_LEFT_28_PENALTY=2.774563
PRIMER_RIGHT_28_PENALTY=0.129335
PRIMER_LEFT_28_SEQUENCE=TTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_28_SEQUENCE=TCCGATGTTTCCTCCATTTC
PRIMER_LEFT_28=635,20
PRIMER_RIGHT_28=1568,20
PRIMER_LEFT_28_TM=57.225
PRIMER_RIGHT_28_TM=59.871
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=934
PRIMER_PAIR_29_PENALTY=2.904706
PRIMER_LEFT_29_PENALTY=2.775371
PRIMER_RIGHT_29_PENALTY=0.129335
PRIMER_LEFT_29_SEQUENCE=TTAGCTAGGAAAAACGCCACT
PRIMER_RIGHT_29_SEQUENCE=TCCGATGTTTCCTCCATTTC
PRIMER_LEFT_29=635,21
PRIMER_RIGHT_29=1568,20
PRIMER_LEFT_29_TM=58.225
PRIMER_RIGHT_29_TM=59.871
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=934
PRIMER_PAIR_30_PENALTY=2.930623
PRIMER_LEFT_30_PENALTY=2.774563
PRIMER_RIGHT_30_PENALTY=0.156061
PRIMER_LEFT_30_SEQUENCE=TTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_30_SEQUENCE=CCTTGAGCTGGAACTGGAAC
PRIMER_LEFT_30=635,20
PRIMER_RIGHT_30=1610,20
PRIMER_LEFT_30_TM=57.225
PRIMER_RIGHT_30_TM=59.844
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=976
PRIMER_PAIR_31_PENALTY=2.931431
PRIMER_LEFT_31_PENALTY=2.775371
PRIMER_RIGHT_31_PENALTY=0.156061
PRIMER_LEFT_31_SEQUENCE=TTAGCTAGGAAAAACGCCACT
PRIMER_RIGHT_31_SEQUENCE=CCTTGAGCTGGAACTGGAAC
PRIMER_LEFT_31=635,21
PRIMER_RIGHT_31=1610,20
PRIMER_LEFT_31_TM=58.225
PRIMER_RIGHT_31_TM=59.844
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=976
PRIMER_PAIR_32_PENALTY=2.933889
PRIMER_LEFT_32_PENALTY=2.147383
PRIMER_RIGHT_32_PENALTY=0.786506
PRIMER_LEFT_32_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_32_SEQUENCE=TTCTCTCCGATGTTTCCTCC
PRIMER_LEFT_32=633,22
PRIMER_RIGHT_32=1573,20
PRIMER_LEFT_32_TM=60.147
PRIMER_RIGHT_32_TM=59.213
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=941
PRIMER_PAIR_33_PENALTY=2.933889
PRIMER_LEFT_33_PENALTY=2.147383
PRIMER_RIGHT_33_PENALTY=0.786506
PRIMER_LEFT_33_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_33_SEQUENCE=TCCTTCTCTCCGATGTTTCC
PRIMER_LEFT_33=633,22
PRIMER_RIGHT_33=1576,20
PRIMER_LEFT_33_TM=60.147
PRIMER_RIGHT_33_TM=59.213
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=944
PRIMER_PAIR_34_PENALTY=2.984770
PRIMER_LEFT_34_PENALTY=2.468095
PRIMER_RIGHT_34_PENALTY=0.516675
PRIMER_LEFT_34_SEQUENCE=TCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_34_SEQUENCE=TCTCCGATGTTTCCTCCATT
PRIMER_LEFT_34=632,21
PRIMER_RIGHT_34=1570,20
PRIMER_LEFT_34_TM=58.532
PRIMER_RIGHT_34_TM=59.483
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=11.1000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=939
PRIMER_PAIR_35_PENALTY=2.996284
PRIMER_LEFT_35_PENALTY=1.606344
PRIMER_RIGHT_35_PENALTY=1.389940
PRIMER_LEFT_35_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_35_SEQUENCE=TGTCCTTGAGCTGGAACTGG
PRIMER_LEFT_35=633,21
PRIMER_RIGHT_35=1613,20
PRIMER_LEFT_35_TM=59.394
PRIMER_RIGHT_35_TM=61.390
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=11.7000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=981
PRIMER_PAIR_36_PENALTY=3.035124
PRIMER_LEFT_36_PENALTY=1.606344
PRIMER_RIGHT_36_PENALTY=1.428780
PRIMER_LEFT_36_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_36_SEQUENCE=TCCTCCATTTCCGATTTCTT
PRIMER_LEFT_36=633,21
PRIMER_RIGHT_36=1559,20
PRIMER_LEFT_36_TM=59.394
PRIMER_RIGHT_36_TM=58.571
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=11.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=927
PRIMER_PAIR_37_PENALTY=3.035124
PRIMER_LEFT_37_PENALTY=1.606344
PRIMER_RIGHT_37_PENALTY=1.428780
PRIMER_LEFT_37_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_37_SEQUENCE=TTCCTCCATTTCCGATTTCT
PRIMER_LEFT_37=633,21
PRIMER_RIGHT_37=1560,20
PRIMER_LEFT_37_TM=59.394
PRIMER_RIGHT_37_TM=58.571
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=11.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=928
PRIMER_PAIR_38_PENALTY=3.055353
PRIMER_LEFT_38_PENALTY=1.606344
PRIMER_RIGHT_38_PENALTY=1.449009
PRIMER_LEFT_38_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_38_SEQUENCE=CTCTCCGATGTTTCCTCCATT
PRIMER_LEFT_38=633,21
PRIMER_RIGHT_38=1571,21
PRIMER_LEFT_38_TM=59.394
PRIMER_RIGHT_38_TM=60.449
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=11.7000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=939
PRIMER_PAIR_39_PENALTY=3.060084
PRIMER_LEFT_39_PENALTY=2.147383
PRIMER_RIGHT_39_PENALTY=0.912701
PRIMER_LEFT_39_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_39_SEQUENCE=TCCTTGAGCTGGAACTGGAA
PRIMER_LEFT_39=633,22
PRIMER_RIGHT_39=1611,20
PRIMER_LEFT_39_TM=60.147
PRIMER_RIGHT_39_TM=60.913
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=979
PRIMER_PAIR_40_PENALTY=3.070976
PRIMER_LEFT_40_PENALTY=2.685220
PRIMER_RIGHT_40_PENALTY=0.385756
PRIMER_LEFT_40_SEQUENCE=GTCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_40_SEQUENCE=GTCCTTGAGCTGGAACTGGA
PRIMER_LEFT_40=631,22
PRIMER_RIGHT_40=1612,20
PRIMER_LEFT_40_TM=59.315
PRIMER_RIGHT_40_TM=60.386
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.1000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=982
PRIMER_PAIR_41_PENALTY=3.094510
PRIMER_LEFT_41_PENALTY=2.468095
PRIMER_RIGHT_41_PENALTY=0.626415
PRIMER_LEFT_41_SEQUENCE=TCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_41_SEQUENCE=CGATTTCTTTGTCCTCACGG
PRIMER_LEFT_41=632,21
PRIMER_RIGHT_41=1548,20
PRIMER_LEFT_41_TM=58.532
PRIMER_RIGHT_41_TM=60.626
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=11.1000
PRIMER_RIGHT_41_END_STABILITY=9.9000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=917
PRIMER_PAIR_42_PENALTY=3.105056
PRIMER_LEFT_42_PENALTY=2.468095
PRIMER_RIGHT_42_PENALTY=0.636961
PRIMER_LEFT_42_SEQUENCE=TCATTAGCTAGGAAAAACGCC
PRIMER_RIGHT_42_SEQUENCE=TGTTTCCTCCATTTCCGATT
PRIMER_LEFT_42=632,21
PRIMER_RIGHT_42=1563,20
PRIMER_LEFT_42_TM=58.532
PRIMER_RIGHT_42_TM=59.363
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=11.1000
PRIMER_RIGHT_42_END_STABILITY=8.6000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=932
PRIMER_PAIR_43_PENALTY=3.160319
PRIMER_LEFT_43_PENALTY=2.774563
PRIMER_RIGHT_43_PENALTY=0.385756
PRIMER_LEFT_43_SEQUENCE=TTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_43_SEQUENCE=GTCCTTGAGCTGGAACTGGA
PRIMER_LEFT_43=635,20
PRIMER_RIGHT_43=1612,20
PRIMER_LEFT_43_TM=57.225
PRIMER_RIGHT_43_TM=60.386
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=978
PRIMER_PAIR_44_PENALTY=3.161126
PRIMER_LEFT_44_PENALTY=2.775371
PRIMER_RIGHT_44_PENALTY=0.385756
PRIMER_LEFT_44_SEQUENCE=TTAGCTAGGAAAAACGCCACT
PRIMER_RIGHT_44_SEQUENCE=GTCCTTGAGCTGGAACTGGA
PRIMER_LEFT_44=635,21
PRIMER_RIGHT_44=1612,20
PRIMER_LEFT_44_TM=58.225
PRIMER_RIGHT_44_TM=60.386
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=978
PRIMER_PAIR_45_PENALTY=3.166095
PRIMER_LEFT_45_PENALTY=2.147383
PRIMER_RIGHT_45_PENALTY=1.018712
PRIMER_LEFT_45_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_45_SEQUENCE=CTCCGATGTTTCCTCCATTT
PRIMER_LEFT_45=633,22
PRIMER_RIGHT_45=1569,20
PRIMER_LEFT_45_TM=60.147
PRIMER_RIGHT_45_TM=58.981
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=7.2000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=937
PRIMER_PAIR_46_PENALTY=3.171012
PRIMER_LEFT_46_PENALTY=1.606344
PRIMER_RIGHT_46_PENALTY=1.564669
PRIMER_LEFT_46_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_46_SEQUENCE=GTGTCCTTGAGCTGGAACTG
PRIMER_LEFT_46=633,21
PRIMER_RIGHT_46=1614,20
PRIMER_LEFT_46_TM=59.394
PRIMER_RIGHT_46_TM=58.435
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=11.7000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=982
PRIMER_PAIR_47_PENALTY=3.180729
PRIMER_LEFT_47_PENALTY=1.606344
PRIMER_RIGHT_47_PENALTY=1.574386
PRIMER_LEFT_47_SEQUENCE=CATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_47_SEQUENCE=CCGATGTTTCCTCCATTTCC
PRIMER_LEFT_47=633,21
PRIMER_RIGHT_47=1567,20
PRIMER_LEFT_47_TM=59.394
PRIMER_RIGHT_47_TM=61.574
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=11.7000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=935
PRIMER_PAIR_48_PENALTY=3.184121
PRIMER_LEFT_48_PENALTY=2.147383
PRIMER_RIGHT_48_PENALTY=1.036738
PRIMER_LEFT_48_SEQUENCE=CATTAGCTAGGAAAAACGCCAC
PRIMER_RIGHT_48_SEQUENCE=CCTTCTCCTTCTCTCCGATG
PRIMER_LEFT_48=633,22
PRIMER_RIGHT_48=1581,20
PRIMER_LEFT_48_TM=60.147
PRIMER_RIGHT_48_TM=58.963
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=8.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=949
PRIMER_PAIR_49_PENALTY=3.189324
PRIMER_LEFT_49_PENALTY=3.059989
PRIMER_RIGHT_49_PENALTY=0.129335
PRIMER_LEFT_49_SEQUENCE=TCATTAGCTAGGAAAAACGCCA
PRIMER_RIGHT_49_SEQUENCE=TCCGATGTTTCCTCCATTTC
PRIMER_LEFT_49=632,22
PRIMER_RIGHT_49=1568,20
PRIMER_LEFT_49_TM=61.060
PRIMER_RIGHT_49_TM=59.871
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=11.7000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=937
=
SEQUENCE_ID=MAGIC.105_3_13955872_13956316_wil-2_wu-0_54584_3822_TRUE
SEQUENCE_TARGET=950,544
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4643191_4643690_bur-0_rsch-4_8812_58699_TRUE
SEQUENCE_TARGET=950,599
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNAAAGAATAAANAGAATAGNCNTTNACGAATANTATGANAGNNNAAAAAAACNTTTGCACNTNATTTATGACANTTTTACNCTAAANAAAAGNCAAACCNTCNTTNTTCCTNCTTCCATNTCTTTCTCTTTCTNTTTCTTNTTTCGACAAANTACATCTCTATTTTCTACCATTGTGCCTTATTCTTTGATNTCTNANANCATCATCACCTTCTCCATAACCACCTCCATTACAACAACCACCACAAGCTCTAGTAGATCATNTGACTATAGCCGTNAACNGTGTTGNTTTTCATCAATTTGGGATTCNGTGGTTCAACCATTGAGNTNTTCNGTTTATTTGGTCTATATANNATTTAGCTATTTCNGATATCGTTTCTTTTTANAATCTGAGATTTGCNTTCACCCTCAAGAGTTCAAGGTTTCNTTTCAATGTTATGTTATCATTTCATATATCANGTTANTTTNANNTTAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGTCTANTACCCCCCNNNNTTNAAAACCACTCGCTCNAAAANTCCTCTCAAACACTATAATCGAGACAANTNCNTTNACCATGTTTTCTGANGCCGNTAGAGTTTTTGCTCGNCGGCGTCGNGATATTGTTTGCTTTTCTCAATCCTTTCGCAACCTTTCTNGATTCTTACTTCATTTGTTTATAATCTTNTACCATNNNNNNNNNCTTTCACCTTGCTTCCATGGTGTTCCTTTGTACCGCGATAACATTAATAGAGGTTTTCNGGCGAACCCTNTATCCCTCATNAGGCCCGTGAACTCNGTGAAGGAGGAACCNATGTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_7627778_7628126_ct-1_po-0_6588_20614_TRUE
SEQUENCE_TARGET=950,448
SEQUENCE_TEMPLATE=GCATTCTTTTCAGCATTGGTGAAACAACCAGAGTTGTTNAAGCCTTGAAAATTAGCCTTAAANGGAGCCAAAGACCAATTAATCTTGCTTTTACCACCGTCTGTCGCCCAGTTTTCACCGTTCCACAAGCTAACCACAACTTGCATAGGTTTTGATGGGTAGTTCATGCCTTGGCTTGTGGTGTTCTTGAACACCCGTACNGGAATGTTGTCCACATAAAGCCTATTGGTAATCAACACATGCTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAACGAGTGGGACGTACACAATTTGATACGGGTTCCACAGAATTGCGTAAGTGTGGANATCTTTAGAAGGATCGAACCAGAGAGCTAACTTCTGTTCTCTATNNCCTTTACCATTAGTAAACACATTTGTTTGCACTGNTAGTTTCCCTTCTTTGTTTCCCAAGAATTCAAAATCAACTTCATCATGTGTGTTTCCCTTCGAAGTCAACTGTTTNCCAATACACAAATATATTATATACAAAANNTAANATATAACTTTATGTTATCCAGATNAACATNAAANNNGGTCAAGACTAGAACATGGTNNAACNACTACNGACTTANACCGTTTAATTAANTACCNTTAAGNCACACGCAAGTTNAAAATTAAAATATGAACAAANAAATGCTAGTAGACNTNTTTTTTNTGTAAAANAANTTCNNTTCAAATTNATGTAAAATGAAAAATAANATTGCTACAATGCNGAGATAAAATTCAAACTTACATAGAAAGCGGTGACAACGCCTGAGGTATCTTTTGGAGGCACTTTGATTCTTATTTGGANGAATCCTGATTCGTNATGATTCTTAGATTCAAAACCAGAACCTGNTTTTGTAGTTTACGTAGAAATCAATANAAAGGATGGTCTTTNNNNCAAACGATTTAAANTTATTNANANAAAAATTCAATGGACCTGAAGAATGATCNAATGAAAGCTGAACTTCTTTCCCTTGGTTAAGTTTCAAGACATTATTTTGTCCCCACGTAACGACNTAGTTATCNTCGAAACCTACTTTGCTTTGGTCGATTCCNTTAACTTCATAAGCCCTAGCATCAACCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.308006
PRIMER_LEFT_0_PENALTY=0.482208
PRIMER_RIGHT_0_PENALTY=0.825798
PRIMER_LEFT_0_SEQUENCE=TTCCCTTCTTTGTTTCCCAA
PRIMER_RIGHT_0_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_0=860,20
PRIMER_RIGHT_0=1508,20
PRIMER_LEFT_0_TM=59.518
PRIMER_RIGHT_0_TM=59.174
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=649
PRIMER_PAIR_1_PENALTY=1.308006
PRIMER_LEFT_1_PENALTY=0.482208
PRIMER_RIGHT_1_PENALTY=0.825798
PRIMER_LEFT_1_SEQUENCE=TTTCCCTTCTTTGTTTCCCA
PRIMER_RIGHT_1_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_1=859,20
PRIMER_RIGHT_1=1508,20
PRIMER_LEFT_1_TM=59.518
PRIMER_RIGHT_1_TM=59.174
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=650
PRIMER_PAIR_2_PENALTY=1.813538
PRIMER_LEFT_2_PENALTY=1.027381
PRIMER_RIGHT_2_PENALTY=0.786156
PRIMER_LEFT_2_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_2_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_2=738,20
PRIMER_RIGHT_2=1469,20
PRIMER_LEFT_2_TM=58.973
PRIMER_RIGHT_2_TM=60.786
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=732
PRIMER_PAIR_3_PENALTY=1.853179
PRIMER_LEFT_3_PENALTY=1.027381
PRIMER_RIGHT_3_PENALTY=0.825798
PRIMER_LEFT_3_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_3_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_3=738,20
PRIMER_RIGHT_3=1508,20
PRIMER_LEFT_3_TM=58.973
PRIMER_RIGHT_3_TM=59.174
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=771
PRIMER_PAIR_4_PENALTY=2.282996
PRIMER_LEFT_4_PENALTY=1.027381
PRIMER_RIGHT_4_PENALTY=1.255615
PRIMER_LEFT_4_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_4_SEQUENCE=CGTTACGTGGGGACAAAATAA
PRIMER_LEFT_4=738,20
PRIMER_RIGHT_4=1467,21
PRIMER_LEFT_4_TM=58.973
PRIMER_RIGHT_4_TM=59.744
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=730
PRIMER_PAIR_5_PENALTY=2.483862
PRIMER_LEFT_5_PENALTY=1.697705
PRIMER_RIGHT_5_PENALTY=0.786156
PRIMER_LEFT_5_SEQUENCE=ATTTGATACGGGTTCCACAGA
PRIMER_RIGHT_5_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_5=737,21
PRIMER_RIGHT_5=1469,20
PRIMER_LEFT_5_TM=59.302
PRIMER_RIGHT_5_TM=60.786
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=733
PRIMER_PAIR_6_PENALTY=2.523503
PRIMER_LEFT_6_PENALTY=1.697705
PRIMER_RIGHT_6_PENALTY=0.825798
PRIMER_LEFT_6_SEQUENCE=ATTTGATACGGGTTCCACAGA
PRIMER_RIGHT_6_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_6=737,21
PRIMER_RIGHT_6=1508,20
PRIMER_LEFT_6_TM=59.302
PRIMER_RIGHT_6_TM=59.174
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=772
PRIMER_PAIR_7_PENALTY=2.611311
PRIMER_LEFT_7_PENALTY=0.482208
PRIMER_RIGHT_7_PENALTY=2.129103
PRIMER_LEFT_7_SEQUENCE=TTTCCCTTCTTTGTTTCCCA
PRIMER_RIGHT_7_SEQUENCE=GGAATCGACCAAAGCAAAGTAG
PRIMER_LEFT_7=859,20
PRIMER_RIGHT_7=1508,22
PRIMER_LEFT_7_TM=59.518
PRIMER_RIGHT_7_TM=60.129
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=5.4000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=650
PRIMER_PAIR_8_PENALTY=2.949815
PRIMER_LEFT_8_PENALTY=2.163658
PRIMER_RIGHT_8_PENALTY=0.786156
PRIMER_LEFT_8_SEQUENCE=TTCATCATGTGTGTTTCCCTTC
PRIMER_RIGHT_8_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_8=895,22
PRIMER_RIGHT_8=1469,20
PRIMER_LEFT_8_TM=59.836
PRIMER_RIGHT_8_TM=60.786
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=575
PRIMER_PAIR_9_PENALTY=2.953320
PRIMER_LEFT_9_PENALTY=1.697705
PRIMER_RIGHT_9_PENALTY=1.255615
PRIMER_LEFT_9_SEQUENCE=ATTTGATACGGGTTCCACAGA
PRIMER_RIGHT_9_SEQUENCE=CGTTACGTGGGGACAAAATAA
PRIMER_LEFT_9=737,21
PRIMER_RIGHT_9=1467,21
PRIMER_LEFT_9_TM=59.302
PRIMER_RIGHT_9_TM=59.744
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=731
PRIMER_PAIR_10_PENALTY=2.963338
PRIMER_LEFT_10_PENALTY=2.177182
PRIMER_RIGHT_10_PENALTY=0.786156
PRIMER_LEFT_10_SEQUENCE=AATTTGATACGGGTTCCACAG
PRIMER_RIGHT_10_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_10=736,21
PRIMER_RIGHT_10=1469,20
PRIMER_LEFT_10_TM=58.823
PRIMER_RIGHT_10_TM=60.786
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=734
PRIMER_PAIR_11_PENALTY=2.989456
PRIMER_LEFT_11_PENALTY=2.163658
PRIMER_RIGHT_11_PENALTY=0.825798
PRIMER_LEFT_11_SEQUENCE=TTCATCATGTGTGTTTCCCTTC
PRIMER_RIGHT_11_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_11=895,22
PRIMER_RIGHT_11=1508,20
PRIMER_LEFT_11_TM=59.836
PRIMER_RIGHT_11_TM=59.174
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=614
PRIMER_PAIR_12_PENALTY=2.993111
PRIMER_LEFT_12_PENALTY=2.206954
PRIMER_RIGHT_12_PENALTY=0.786156
PRIMER_LEFT_12_SEQUENCE=CAATTTGATACGGGTTCCAC
PRIMER_RIGHT_12_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_12=735,20
PRIMER_RIGHT_12=1469,20
PRIMER_LEFT_12_TM=57.793
PRIMER_RIGHT_12_TM=60.786
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=735
PRIMER_PAIR_13_PENALTY=2.998767
PRIMER_LEFT_13_PENALTY=0.482208
PRIMER_RIGHT_13_PENALTY=2.516559
PRIMER_LEFT_13_SEQUENCE=TTTCCCTTCTTTGTTTCCCA
PRIMER_RIGHT_13_SEQUENCE=TCGACCAAAGCAAAGTAGGT
PRIMER_LEFT_13=859,20
PRIMER_RIGHT_13=1504,20
PRIMER_LEFT_13_TM=59.518
PRIMER_RIGHT_13_TM=57.483
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=8.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=646
PRIMER_PAIR_14_PENALTY=3.002980
PRIMER_LEFT_14_PENALTY=2.177182
PRIMER_RIGHT_14_PENALTY=0.825798
PRIMER_LEFT_14_SEQUENCE=AATTTGATACGGGTTCCACAG
PRIMER_RIGHT_14_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_14=736,21
PRIMER_RIGHT_14=1508,20
PRIMER_LEFT_14_TM=58.823
PRIMER_RIGHT_14_TM=59.174
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=773
PRIMER_PAIR_15_PENALTY=3.032752
PRIMER_LEFT_15_PENALTY=2.206954
PRIMER_RIGHT_15_PENALTY=0.825798
PRIMER_LEFT_15_SEQUENCE=CAATTTGATACGGGTTCCAC
PRIMER_RIGHT_15_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_15=735,20
PRIMER_RIGHT_15=1508,20
PRIMER_LEFT_15_TM=57.793
PRIMER_RIGHT_15_TM=59.174
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=774
PRIMER_PAIR_16_PENALTY=3.102190
PRIMER_LEFT_16_PENALTY=2.316034
PRIMER_RIGHT_16_PENALTY=0.786156
PRIMER_LEFT_16_SEQUENCE=ACAATTTGATACGGGTTCCAC
PRIMER_RIGHT_16_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_16=734,21
PRIMER_RIGHT_16=1469,20
PRIMER_LEFT_16_TM=58.684
PRIMER_RIGHT_16_TM=60.786
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=736
PRIMER_PAIR_17_PENALTY=3.141832
PRIMER_LEFT_17_PENALTY=2.316034
PRIMER_RIGHT_17_PENALTY=0.825798
PRIMER_LEFT_17_SEQUENCE=ACAATTTGATACGGGTTCCAC
PRIMER_RIGHT_17_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_17=734,21
PRIMER_RIGHT_17=1508,20
PRIMER_LEFT_17_TM=58.684
PRIMER_RIGHT_17_TM=59.174
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=775
PRIMER_PAIR_18_PENALTY=3.156485
PRIMER_LEFT_18_PENALTY=1.027381
PRIMER_RIGHT_18_PENALTY=2.129103
PRIMER_LEFT_18_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_18_SEQUENCE=GAATCGACCAAAGCAAAGTAGG
PRIMER_LEFT_18=738,20
PRIMER_RIGHT_18=1507,22
PRIMER_LEFT_18_TM=58.973
PRIMER_RIGHT_18_TM=60.129
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=770
PRIMER_PAIR_19_PENALTY=3.156485
PRIMER_LEFT_19_PENALTY=1.027381
PRIMER_RIGHT_19_PENALTY=2.129103
PRIMER_LEFT_19_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_19_SEQUENCE=GGAATCGACCAAAGCAAAGTAG
PRIMER_LEFT_19=738,20
PRIMER_RIGHT_19=1508,22
PRIMER_LEFT_19_TM=58.973
PRIMER_RIGHT_19_TM=60.129
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=5.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=771
PRIMER_PAIR_20_PENALTY=3.212318
PRIMER_LEFT_20_PENALTY=2.386520
PRIMER_RIGHT_20_PENALTY=0.825798
PRIMER_LEFT_20_SEQUENCE=GTTTCCCTTCTTTGTTTCCC
PRIMER_RIGHT_20_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_20=858,20
PRIMER_RIGHT_20=1508,20
PRIMER_LEFT_20_TM=57.613
PRIMER_RIGHT_20_TM=59.174
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=1.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=651
PRIMER_PAIR_21_PENALTY=3.281977
PRIMER_LEFT_21_PENALTY=2.495821
PRIMER_RIGHT_21_PENALTY=0.786156
PRIMER_LEFT_21_SEQUENCE=AAGGATCGAACCAGAGAGCTAA
PRIMER_RIGHT_21_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_21=783,22
PRIMER_RIGHT_21=1469,20
PRIMER_LEFT_21_TM=59.504
PRIMER_RIGHT_21_TM=60.786
PRIMER_LEFT_21_GC_PERCENT=45.455
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.5000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=687
PRIMER_PAIR_22_PENALTY=3.282587
PRIMER_LEFT_22_PENALTY=0.482208
PRIMER_RIGHT_22_PENALTY=2.800379
PRIMER_LEFT_22_SEQUENCE=TTTCCCTTCTTTGTTTCCCA
PRIMER_RIGHT_22_SEQUENCE=AATCGACCAAAGCAAAGTAGGT
PRIMER_LEFT_22=859,20
PRIMER_RIGHT_22=1506,22
PRIMER_LEFT_22_TM=59.518
PRIMER_RIGHT_22_TM=59.200
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=8.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=648
PRIMER_PAIR_23_PENALTY=3.321619
PRIMER_LEFT_23_PENALTY=2.495821
PRIMER_RIGHT_23_PENALTY=0.825798
PRIMER_LEFT_23_SEQUENCE=AAGGATCGAACCAGAGAGCTAA
PRIMER_RIGHT_23_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_23=783,22
PRIMER_RIGHT_23=1508,20
PRIMER_LEFT_23_TM=59.504
PRIMER_RIGHT_23_TM=59.174
PRIMER_LEFT_23_GC_PERCENT=45.455
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.5000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=726
PRIMER_PAIR_24_PENALTY=3.378837
PRIMER_LEFT_24_PENALTY=2.592680
PRIMER_RIGHT_24_PENALTY=0.786156
PRIMER_LEFT_24_SEQUENCE=AATTTGATACGGGTTCCACAGA
PRIMER_RIGHT_24_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_24=736,22
PRIMER_RIGHT_24=1469,20
PRIMER_LEFT_24_TM=60.593
PRIMER_RIGHT_24_TM=60.786
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=734
PRIMER_PAIR_25_PENALTY=3.384778
PRIMER_LEFT_25_PENALTY=2.598622
PRIMER_RIGHT_25_PENALTY=0.786156
PRIMER_LEFT_25_SEQUENCE=TCATCATGTGTGTTTCCCTTC
PRIMER_RIGHT_25_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_25=896,21
PRIMER_RIGHT_25=1469,20
PRIMER_LEFT_25_TM=58.401
PRIMER_RIGHT_25_TM=60.786
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=574
PRIMER_PAIR_26_PENALTY=3.393130
PRIMER_LEFT_26_PENALTY=2.606973
PRIMER_RIGHT_26_PENALTY=0.786156
PRIMER_LEFT_26_SEQUENCE=ATTTGATACGGGTTCCACAG
PRIMER_RIGHT_26_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_26=737,20
PRIMER_RIGHT_26=1469,20
PRIMER_LEFT_26_TM=57.393
PRIMER_RIGHT_26_TM=60.786
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=733
PRIMER_PAIR_27_PENALTY=3.418478
PRIMER_LEFT_27_PENALTY=2.592680
PRIMER_RIGHT_27_PENALTY=0.825798
PRIMER_LEFT_27_SEQUENCE=AATTTGATACGGGTTCCACAGA
PRIMER_RIGHT_27_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_27=736,22
PRIMER_RIGHT_27=1508,20
PRIMER_LEFT_27_TM=60.593
PRIMER_RIGHT_27_TM=59.174
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=773
PRIMER_PAIR_28_PENALTY=3.419273
PRIMER_LEFT_28_PENALTY=2.163658
PRIMER_RIGHT_28_PENALTY=1.255615
PRIMER_LEFT_28_SEQUENCE=TTCATCATGTGTGTTTCCCTTC
PRIMER_RIGHT_28_SEQUENCE=CGTTACGTGGGGACAAAATAA
PRIMER_LEFT_28=895,22
PRIMER_RIGHT_28=1467,21
PRIMER_LEFT_28_TM=59.836
PRIMER_RIGHT_28_TM=59.744
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=573
PRIMER_PAIR_29_PENALTY=3.424420
PRIMER_LEFT_29_PENALTY=2.598622
PRIMER_RIGHT_29_PENALTY=0.825798
PRIMER_LEFT_29_SEQUENCE=TCATCATGTGTGTTTCCCTTC
PRIMER_RIGHT_29_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_29=896,21
PRIMER_RIGHT_29=1508,20
PRIMER_LEFT_29_TM=58.401
PRIMER_RIGHT_29_TM=59.174
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=613
PRIMER_PAIR_30_PENALTY=3.432771
PRIMER_LEFT_30_PENALTY=2.606973
PRIMER_RIGHT_30_PENALTY=0.825798
PRIMER_LEFT_30_SEQUENCE=ATTTGATACGGGTTCCACAG
PRIMER_RIGHT_30_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_30=737,20
PRIMER_RIGHT_30=1508,20
PRIMER_LEFT_30_TM=57.393
PRIMER_RIGHT_30_TM=59.174
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=772
PRIMER_PAIR_31_PENALTY=3.432796
PRIMER_LEFT_31_PENALTY=2.177182
PRIMER_RIGHT_31_PENALTY=1.255615
PRIMER_LEFT_31_SEQUENCE=AATTTGATACGGGTTCCACAG
PRIMER_RIGHT_31_SEQUENCE=CGTTACGTGGGGACAAAATAA
PRIMER_LEFT_31=736,21
PRIMER_RIGHT_31=1467,21
PRIMER_LEFT_31_TM=58.823
PRIMER_RIGHT_31_TM=59.744
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=6.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=732
PRIMER_PAIR_32_PENALTY=3.439769
PRIMER_LEFT_32_PENALTY=2.653612
PRIMER_RIGHT_32_PENALTY=0.786156
PRIMER_LEFT_32_SEQUENCE=CAGAATTGCGTAAGTGTGGA
PRIMER_RIGHT_32_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_32=754,20
PRIMER_RIGHT_32=1469,20
PRIMER_LEFT_32_TM=57.346
PRIMER_RIGHT_32_TM=60.786
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=716
PRIMER_PAIR_33_PENALTY=3.447176
PRIMER_LEFT_33_PENALTY=1.318073
PRIMER_RIGHT_33_PENALTY=2.129103
PRIMER_LEFT_33_SEQUENCE=GTTTCCCTTCTTTGTTTCCCA
PRIMER_RIGHT_33_SEQUENCE=GGAATCGACCAAAGCAAAGTAG
PRIMER_LEFT_33=858,21
PRIMER_RIGHT_33=1508,22
PRIMER_LEFT_33_TM=60.318
PRIMER_RIGHT_33_TM=60.129
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=5.4000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=651
PRIMER_PAIR_34_PENALTY=3.462569
PRIMER_LEFT_34_PENALTY=2.206954
PRIMER_RIGHT_34_PENALTY=1.255615
PRIMER_LEFT_34_SEQUENCE=CAATTTGATACGGGTTCCAC
PRIMER_RIGHT_34_SEQUENCE=CGTTACGTGGGGACAAAATAA
PRIMER_LEFT_34=735,20
PRIMER_RIGHT_34=1467,21
PRIMER_LEFT_34_TM=57.793
PRIMER_RIGHT_34_TM=59.744
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=6.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=733
PRIMER_PAIR_35_PENALTY=3.479410
PRIMER_LEFT_35_PENALTY=2.653612
PRIMER_RIGHT_35_PENALTY=0.825798
PRIMER_LEFT_35_SEQUENCE=CAGAATTGCGTAAGTGTGGA
PRIMER_RIGHT_35_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_35=754,20
PRIMER_RIGHT_35=1508,20
PRIMER_LEFT_35_TM=57.346
PRIMER_RIGHT_35_TM=59.174
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=755
PRIMER_PAIR_36_PENALTY=3.543940
PRIMER_LEFT_36_PENALTY=1.027381
PRIMER_RIGHT_36_PENALTY=2.516559
PRIMER_LEFT_36_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_36_SEQUENCE=TCGACCAAAGCAAAGTAGGT
PRIMER_LEFT_36=738,20
PRIMER_RIGHT_36=1504,20
PRIMER_LEFT_36_TM=58.973
PRIMER_RIGHT_36_TM=57.483
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=767
PRIMER_PAIR_37_PENALTY=3.571648
PRIMER_LEFT_37_PENALTY=2.316034
PRIMER_RIGHT_37_PENALTY=1.255615
PRIMER_LEFT_37_SEQUENCE=ACAATTTGATACGGGTTCCAC
PRIMER_RIGHT_37_SEQUENCE=CGTTACGTGGGGACAAAATAA
PRIMER_LEFT_37=734,21
PRIMER_RIGHT_37=1467,21
PRIMER_LEFT_37_TM=58.684
PRIMER_RIGHT_37_TM=59.744
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=734
PRIMER_PAIR_38_PENALTY=3.602547
PRIMER_LEFT_38_PENALTY=0.482208
PRIMER_RIGHT_38_PENALTY=3.120339
PRIMER_LEFT_38_SEQUENCE=TTTCCCTTCTTTGTTTCCCA
PRIMER_RIGHT_38_SEQUENCE=ATCGACCAAAGCAAAGTAGGT
PRIMER_LEFT_38=859,20
PRIMER_RIGHT_38=1505,21
PRIMER_LEFT_38_TM=59.518
PRIMER_RIGHT_38_TM=57.880
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=8.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=647
PRIMER_PAIR_39_PENALTY=3.625250
PRIMER_LEFT_39_PENALTY=2.839094
PRIMER_RIGHT_39_PENALTY=0.786156
PRIMER_LEFT_39_SEQUENCE=AGGATCGAACCAGAGAGCTAA
PRIMER_RIGHT_39_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_39=784,21
PRIMER_RIGHT_39=1469,20
PRIMER_LEFT_39_TM=58.161
PRIMER_RIGHT_39_TM=60.786
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.5000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=686
PRIMER_PAIR_40_PENALTY=3.637465
PRIMER_LEFT_40_PENALTY=1.027381
PRIMER_RIGHT_40_PENALTY=2.610083
PRIMER_LEFT_40_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_40_SEQUENCE=AATCGACCAAAGCAAAGTAGG
PRIMER_LEFT_40=738,20
PRIMER_RIGHT_40=1506,21
PRIMER_LEFT_40_TM=58.973
PRIMER_RIGHT_40_TM=58.390
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=769
PRIMER_PAIR_41_PENALTY=3.664892
PRIMER_LEFT_41_PENALTY=2.839094
PRIMER_RIGHT_41_PENALTY=0.825798
PRIMER_LEFT_41_SEQUENCE=AGGATCGAACCAGAGAGCTAA
PRIMER_RIGHT_41_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_41=784,21
PRIMER_RIGHT_41=1508,20
PRIMER_LEFT_41_TM=58.161
PRIMER_RIGHT_41_TM=59.174
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.5000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=725
PRIMER_PAIR_42_PENALTY=3.708093
PRIMER_LEFT_42_PENALTY=2.921937
PRIMER_RIGHT_42_PENALTY=0.786156
PRIMER_LEFT_42_SEQUENCE=GGATCGAACCAGAGAGCTAA
PRIMER_RIGHT_42_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_42=785,20
PRIMER_RIGHT_42=1469,20
PRIMER_LEFT_42_TM=57.078
PRIMER_RIGHT_42_TM=60.786
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.5000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=685
PRIMER_PAIR_43_PENALTY=3.747734
PRIMER_LEFT_43_PENALTY=2.921937
PRIMER_RIGHT_43_PENALTY=0.825798
PRIMER_LEFT_43_SEQUENCE=GGATCGAACCAGAGAGCTAA
PRIMER_RIGHT_43_SEQUENCE=GGAATCGACCAAAGCAAAGT
PRIMER_LEFT_43=785,20
PRIMER_RIGHT_43=1508,20
PRIMER_LEFT_43_TM=57.078
PRIMER_RIGHT_43_TM=59.174
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.5000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=724
PRIMER_PAIR_44_PENALTY=3.751435
PRIMER_LEFT_44_PENALTY=2.495821
PRIMER_RIGHT_44_PENALTY=1.255615
PRIMER_LEFT_44_SEQUENCE=AAGGATCGAACCAGAGAGCTAA
PRIMER_RIGHT_44_SEQUENCE=CGTTACGTGGGGACAAAATAA
PRIMER_LEFT_44=783,22
PRIMER_RIGHT_44=1467,21
PRIMER_LEFT_44_TM=59.504
PRIMER_RIGHT_44_TM=59.744
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.5000
PRIMER_RIGHT_44_END_STABILITY=6.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=685
PRIMER_PAIR_45_PENALTY=3.826809
PRIMER_LEFT_45_PENALTY=1.697705
PRIMER_RIGHT_45_PENALTY=2.129103
PRIMER_LEFT_45_SEQUENCE=ATTTGATACGGGTTCCACAGA
PRIMER_RIGHT_45_SEQUENCE=GAATCGACCAAAGCAAAGTAGG
PRIMER_LEFT_45=737,21
PRIMER_RIGHT_45=1507,22
PRIMER_LEFT_45_TM=59.302
PRIMER_RIGHT_45_TM=60.129
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=771
PRIMER_PAIR_46_PENALTY=3.826809
PRIMER_LEFT_46_PENALTY=1.697705
PRIMER_RIGHT_46_PENALTY=2.129103
PRIMER_LEFT_46_SEQUENCE=ATTTGATACGGGTTCCACAGA
PRIMER_RIGHT_46_SEQUENCE=GGAATCGACCAAAGCAAAGTAG
PRIMER_LEFT_46=737,21
PRIMER_RIGHT_46=1508,22
PRIMER_LEFT_46_TM=59.302
PRIMER_RIGHT_46_TM=60.129
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=5.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=772
PRIMER_PAIR_47_PENALTY=3.827761
PRIMER_LEFT_47_PENALTY=1.027381
PRIMER_RIGHT_47_PENALTY=2.800379
PRIMER_LEFT_47_SEQUENCE=TTTGATACGGGTTCCACAGA
PRIMER_RIGHT_47_SEQUENCE=AATCGACCAAAGCAAAGTAGGT
PRIMER_LEFT_47=738,20
PRIMER_RIGHT_47=1506,22
PRIMER_LEFT_47_TM=58.973
PRIMER_RIGHT_47_TM=59.200
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=769
PRIMER_PAIR_48_PENALTY=3.827771
PRIMER_LEFT_48_PENALTY=3.041614
PRIMER_RIGHT_48_PENALTY=0.786156
PRIMER_LEFT_48_SEQUENCE=CTTCATCATGTGTGTTTCCCTT
PRIMER_RIGHT_48_SEQUENCE=GTCGTTACGTGGGGACAAAA
PRIMER_LEFT_48=894,22
PRIMER_RIGHT_48=1469,20
PRIMER_LEFT_48_TM=58.958
PRIMER_RIGHT_48_TM=60.786
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=576
PRIMER_PAIR_49_PENALTY=3.834632
PRIMER_LEFT_49_PENALTY=1.318073
PRIMER_RIGHT_49_PENALTY=2.516559
PRIMER_LEFT_49_SEQUENCE=GTTTCCCTTCTTTGTTTCCCA
PRIMER_RIGHT_49_SEQUENCE=TCGACCAAAGCAAAGTAGGT
PRIMER_LEFT_49=858,21
PRIMER_RIGHT_49=1504,20
PRIMER_LEFT_49_TM=60.318
PRIMER_RIGHT_49_TM=57.483
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=8.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=647
=
SEQUENCE_ID=MAGIC.329_1_11047057_11047319_ws-0_ler-0_1438830_147600_TRUE
SEQUENCE_TARGET=950,362
SEQUENCE_TEMPLATE=AACACNCANTNTTTCNCTTTTAACACTCCACATNNTNTTNTCTNTCTTCCACATCANTATTCNACACCTACATAATTTTTAATNTTACAATAGTTTTGTTTAATAAAATTNCAACANCTTACCCCACCATTTTTATTTCNTATTTTAACCTATACATTATTTNTGGGTCTAAAAATGNTTTTTTCTGTATTTAAATCAAATGAAAATAGTCTNTNTTTATAGAGAANNNNNNNNTATAANANTTTATATNNATNNNNNNNNNNNNAATATANATAAATATTATGAAATAATTGAAGTTATATAATTAANATGAAACAAAAANATTAGAAATCAAAGCAANCAATCAAAATATAACACTTGTCACACTTATCTTCNCCCTTCTACTTTNACNGATGTTNAAATTTATNAACACTAAATTTGCCANATCANNTACTTTTCNATTANAANATACCATTNTANTGGAANTTNTNTTTTCACNANTTGTNAAATNNTTTACNACAAACCATTNTAACTAGTNTAANNNNCTAGAGTCAAGTTCTGGTCCTCGTAAGAGGGNGTGGGTTCAAACCNCACTTCTNACATAANTAACTTAAAATAGACATACTGNNNNNNAAAANAGAAGAAGATTAACCAACAAANTAANTAATCTCTATTACAAAGAAACTTTGACCNNNAAAAGGAATCCCTATAACANATAAGAAGANAGACGATAAGAGNTANGAATTACTTAATTATTAGAAAATATTATAAATAGAGAAANAAACAGAAGAAGANTAACNAAAAAAAGTAACTNGTCTCTATTACAAAGAAACTTTGACCANANANAAANNANGAGCAATTCCTATAACAAATAAGAAGAAGAGACGATAANAGCTAAGAATTAGATAACTATTANAAAAGATTATAAATAGAGGATAGCTATTCATTATTTCTTAAGTACATGAAACACTTGCAACAACAAAAAAGTTAAAGATATNGGTGCTAAATGGAATTCAATGATNATAATGGTGTTTGTGGTGACAATGGTNATAGCAATGGAAAATGTCAATGGAGAGACGANAGCTCAATGCCATGAAGATTGCATTNNANNTTGTGCCACCACAGGAANAATTCCGATCANGTGCCTTCAGAATTGTTACCGTCGATGTCNCGNCAAACCTNGTCGTACATTAGGTNACNATTTTTCAGAAGGTAAAAANAANNNAANAATCTAACAATTTTGNAAATATGTCAAAGAAAATCTATTTTAATGATTATTGAGANTTTNACTAACTATATAAAATATATNCCCCTTTGTATNAATAAATTTGAAGTAAGTTTGANTAGNGNNNTAATAANNANNNNNTAACAATGGANTGTTCAAATGATTNAACGGTATGCNAATNTTCGAAAATGTGGTGAAANATAAAATAGCAACATANCGTTTTGAAANAGTGAAATTTTGAGATATATTTCGGTAATAAAGTGTTTTAAATCTTAGAAACATCATTGTCACTGTTTAGGTATACATATAGTCNTGAAAANATTATTTTTNAAATTAAANTCAAGAGATTATTTGGAAANANTAAAGNTTAAAATAATCGATGGTTCTNATTTGTATAGTGGAAGCGCAGANATAGAAGAGACGATGNAGTCATGNATCAGTGTTGGACTNTTGNGANTTTTAAACTAGCTNTTCTTCTACTTNTGTANNNTCCTTATNTAATATNATCATTGTAANTTTGTAACGCTATTNNNNNNNNNNAAANAAAATTTGTCCCATGTTATAAATTTCTCTNNACTGTAAACANGTCTCTGTTTAACAGTTTAAGACTCCTCTTAGATTCGAATCTCATATTTNATAATTTTGGAAGTGACATGTAGCGGCTTATTGATTTTNTTGATTCATATTTTTGACTTTAGTTATTTCAAAGTGTGATACCTNGTGGATAAAGCAAAATCCNNNNATATGACCATATTTACATGAGACAAAAGTNAACCANCTATGNTTCAGCTNNAAGANAAAAACACTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8594185_8594465_edi-0_po-0_54402_33260_TRUE
SEQUENCE_TARGET=950,380
SEQUENCE_TEMPLATE=GTCTAATCTTAAAAGATCGTCTTTGGTGACTCNTTGCATAGGGACTGTACCAATGGGACAACCCACACCATTCTCCCATAAGTAACCAAAAGCAGTATTGNTNGTCTCTCTTTCTCNCATTTCTTTCCAGATTAGACGCATCTAAGACATGTCGAACACAAACATATCACTGTCCTTTTCTTTTTATGTTTNTAAAANAATATACGTAGATAAAGCTGATCNGNAGTTATTTTATACCTTGTAATGGTATGTGTGATTCTTCATAGAANGATGATCAAATGCTGGTTGCTTGAAGAAATCCACACATCCGTATATTTCTCCATCNATNGTCTATCANTTNATGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGTTAAGATATTNATTGTATNATTAATATATAGAGAACAACANNNAGATTGTGAAGATANAAAGAAACCTTGATGNTCTTGACGGCNNGNTTGTTGATAGTCTGTAGTCTCTTCTCGATTTCCNAATCTTCNGATTTTGAAAAGCTTTTGGCTTCCACTAAGTCATTCTTATGACTTACAATTAAGTGACCAAANATGCAACACATTAAGAACATNCATATCACTGGGCCTCTCACTTCCATTTTTTCAACAANAGAGACAAAAAGAGAGTTTACTGANNTGANNNNNNGACGANACANGTTTAANATATAAGNGTCGAGGAGGCTGAGGNAGGAACATAATAGTTTGTTCTCCTTAATTCAAGACAGTTGACCAAAAATGCTTTTTTGGAAAGACTTTTATGCTGATAATCAATCAAATNGATTCAGTCAACTTATANACATATAATAATNCAGTTGNTTTTTTTAGCAATTGACAAAAAGAATATGTTTTTCTTTTCCAATTATAGCACCACAATTACAAAGTTCCACAAATAACACTTTAGACTTTAATTNAATTTTAAATGTNAATTAATAATTTAACTAAAATGATGANTAAATACNNAAAAAAAAATNAAGGNNNNAAAANANCATGAAATTCAAGAAAGGATTCNTTGAAAGATTCCATGAGTTTCAAAAANTATTCTAAATAATTNAGNAAAAACTCTTCAAACTTNCGAAAATGATTNTTTTCTGAAGTTAATAGGATCCTTCCCTAACTTCATAGAATTCTTTTGGCAGTTCAAGGAATCATTCNTGAATTTTGTGACTTTTNTAGGCNGAAAGATTTTGATGACAATTTGTTTTGACGGAAAAATGTTTTTGGCGGAAAAGTTTATACNNAATTGTTTTGGTCGGGTTAATAGCANGTAATCATGTTTTGACGGNAATTCNTTTTTGTCTTCANATANATATACTTTCTGTTTTTTATTTAACTTAACNAACGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4625569_4625798_wu-0_rsch-4_53205_87063_TRUE
SEQUENCE_TARGET=950,329
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_16436358_16436667_bur-0_zu-0_999_587211_TRUE
SEQUENCE_TARGET=950,409
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGTTTGTTTGGGCTGCAAGGAATGTAAGGAATGTACAATATTCNTAATCGGATNATTGAGNNNNNNNNNNNNNNCTANCAAAATANTTGCTGANTTTCGTGATGATTAGTCATTTGTAATAATTNTGNGATANGTTAAATTCNATAAAATATTGTTAACATTCNTNATTTGATTTTCNTATCNATATAATTNAAGAGACAACGAATTAAAANNTAAANNTGNAAAATATNNTTTAATNTTNNANATNNATAANTNNNNTAANNTTTTNTATGNATNANTNTNNNNTTTTAGGGAAANCATGTNTNNTGNATTNNNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNNACGANATGAANNAANTNTTGAATNANNNNATTNAGTNNTNANNNNNATNNAAANTNTNGAATNNNTTTNNCNTTTTANNNCNNAANNTNTATNNANAAATTNNNNGTANTANNTTTNNTANNTNTTANNTNCACNAANCNNTNNNNAANNNTNNTANTNNANTTNTNTTANCNNNATATTNTNTTANNNACNAAANNCNNNNNNTNANNNNNCNNGTNTTTNATANNNNNNAANNNATNNNNTNAANNTNNNTNAATACNNNANNACTNNNNGTANATNNNTTTATNTTTNAAAANTTATAGAATCANNNTAACNCAATANNNTTNCNNNATNANNNTTTNTGTTTNNNNNTTTNTNGANNNAAANAANNNNANATTNTNNNAATNATTAANATTTNTNNNNNNNNNCATCTTAAANCCNAATANTCTNTCCATNATTAATAAAACAAAACTCCANAAANATTAANACATATCTCAAAATGTTCTATTCTTGATTTTGAAGCNTTCCAAGTGAATCAAGAGCTTCGGTGCGAGTACGAACAATTTGGTGGAAGACATCCCTCACTTTCCTATCAAATGGATCTAAACCCTCCTTTATAGCTTCACACNCATTCATCAATTCTTGAACTCTCTCCTTCACCTCTAANGTANNNTTTTCATCNGTTAATGAGAAATTGTCCGAATCAATCAAATCACTTAACATTCTNGAGTTTCTCTCGATNTGATTAATCTCNCTAAGTAGTCCACAACCCTTCTTCTTTNTGTCTCTTTTCTTTGATTCGTCCATAATCTTATCGTGCAAGGACATGACTGGTACTGCCCATTGGAAATGNCTAGGGAAATAGAAATGGACATGTAAACCACGATCTTGACAAGGTATGGCTGCGACAAGAACCCAAGTNACAAACAACAGTATTGATGTCATTGTATACACTGTGAGAGCTAGGCCATTAGTCGCCATAACATCGCTGGCTCGAGGNGTTGCTAAGTTATTTCCAATGCCTTGTAACTGCCTTGATGCCGACCANGACCGAGAAACACTCCAAGAAAGTGACCTGATATAGCCAATGTGTTGATTATGGTCCTTGTTGCNTGTGAAAGAGCGATTACGGTGNGTGTTAGAAGAGTCTNTCTCGTNGAGCATTCCAATTGCCAAATNAATCAAAGCTTTCTTNGCACGGTGGATTTCTCCTTCTCCAAGTTGTCTTTGGTTCGTGTCCAAAGCCGATATTACNATTTCNATTAGTTTCTGCCACTGTCGGATTTGCTCGATGCCATCNCGTATCGCATTGCANACATCAAGAGCTTTTACACTCCTCTCGAAGTATTCTTCTATCAAGCGGTCCATTGGTTGTTTGAGGAGCTGAGGTTTGTGGTTNAATATTATAACTCGGAAATCTTCTTGGCAACAAAGAAAAGAATCAAGAAGTTTNCCTATCCATTCAAGTGAGAGGATCTCANCNTCATCNGCGGAGGCATTAAGATCGATGAATTTTTCCGCNANTTGTCTTTGAAAAGAATCTAACTCTACCTCCATGGTCATAGGCTCAGAGAAGGATGTTGGATCCATTAAGTGTGCTTGGTCTCGGCGCAAACTAAGGAATGACCTTCCAAACGAATATTCTGTCTCTGGCATCTTTGAAATATANGAAAANTTNGTTTCAAGGGATNAATTTACCTTAGGAAGAAATTGGTGATGAGAATAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_8732096_8732548_wu-0_edi-0_279595_3669_TRUE
SEQUENCE_TARGET=950,552
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTGGGGTTAAATCAGTCCTTCTGCTTTAATTAAGCCGGCGGCTGTCAGGCTAAGTCTGAAAAATCTAGGNTTGGTCCCCNCNTTTTTTTTTTTTTTTTTTTTTTNCTTTTTCAATCTTTTGTCGTATCTATTCATTTCCTAATTTTCTTATAATCGGTGTTGCATCAATCCAAATGTACGGTTGGGATTCTCTTATCCNAAACGCATGCTATTGCTTTGTTGAGGGGACCANNTGTGGTCCAAAGTCCAAGGTCTTGACGCGTGTCCTCTGTCNTTTTTGGCCCGCGAGTGTAGCGACGTGATTTATGGTCNCCTCATCTCTCTNTTAANTTTTTNNCTTCATTACCGGCTATTTCCGGTTGATTGGTTTATGCTAACCGGTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTANNNTATGGTTAAGCAGAATTNTTAATGCGGTTGATACNTAACAAACGAAATTAATATTGTTGATGCATCTAGGTTATTAGGNTCGNNNCNTNNNNNNNNNNNNNNNNNNNNNNGTAAAANNNNNNNNNNNNNTTNANTAAAACCTTGAGTTGTCNACCNAAAAAGAAAGAAAAAGTNNNNNNTAAGTGTATAACNTTAAGTTATGGACNNNTTATGGGTATGTGGAGGGGAANGTAGTTTCTAAAATTGTTCATCAATATTGNNTATATTGNTTTGTTAGTTATAAACNGATANTATTTTAGTGATGATANAGGAGAGNAAANNAAGTAAAGATTTTTGGNAAANCATNAANAAAACAAATGNGNATTAGTGTATGACANANTTAAAAAGGTTAGAGAGGGATGTCAAAAGTGATTAAGAGATAAGAAATCTTCGAAGTTGATTGGTTCTTTTTTCGACTTCNATCAATACTTCTCCTTTTTGTTGATGTTGTTCTTCANANACACACTTCNTTGTTTTTTCTTTCATTCTTATGTAGTTTTTAGTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAATNANNNNNNNAANNTNANATNTNCNNNACANNTNNNANNNTTNNNACNACAAGCAATTGATTTAATTTAATTTATNANNNNNNNNNNNNNNNNNNGATTATGATTTTCTNACGCTCCTCGGANTGACGTGGAAANCACGCTCCAACATTTACGGGGGTTGNTACGTATCGNGTCGCTTTAGATTTNNNGGTTGCTNATTGTTTAGGGATTGTNTATTAGTTTTCCGGTTACTATCCATTTGNATCCAAATAACTCAATAAACCTAAGTATGACTCACTTGTAGGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.802501
PRIMER_LEFT_0_PENALTY=0.411756
PRIMER_RIGHT_0_PENALTY=4.390745
PRIMER_LEFT_0_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_0_SEQUENCE=CACTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_0=929,20
PRIMER_RIGHT_0=1593,24
PRIMER_LEFT_0_TM=60.412
PRIMER_RIGHT_0_TM=60.391
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.833
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=665
PRIMER_PAIR_1_PENALTY=5.017292
PRIMER_LEFT_1_PENALTY=0.411756
PRIMER_RIGHT_1_PENALTY=4.605536
PRIMER_LEFT_1_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_1_SEQUENCE=TCACTTTTGACATCCCTCTCT
PRIMER_LEFT_1=929,20
PRIMER_RIGHT_1=1594,21
PRIMER_LEFT_1_TM=60.412
PRIMER_RIGHT_1_TM=56.394
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=666
PRIMER_PAIR_2_PENALTY=5.158394
PRIMER_LEFT_2_PENALTY=0.411756
PRIMER_RIGHT_2_PENALTY=4.746638
PRIMER_LEFT_2_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_2_SEQUENCE=AATCACTTTTGACATCCCTCTC
PRIMER_LEFT_2=929,20
PRIMER_RIGHT_2=1596,22
PRIMER_LEFT_2_TM=60.412
PRIMER_RIGHT_2_TM=57.253
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=668
PRIMER_PAIR_3_PENALTY=5.260235
PRIMER_LEFT_3_PENALTY=0.411756
PRIMER_RIGHT_3_PENALTY=4.848479
PRIMER_LEFT_3_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_3_SEQUENCE=TCACTTTTGACATCCCTCTC
PRIMER_LEFT_3=929,20
PRIMER_RIGHT_3=1594,20
PRIMER_LEFT_3_TM=60.412
PRIMER_RIGHT_3_TM=55.152
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=666
PRIMER_PAIR_4_PENALTY=5.431393
PRIMER_LEFT_4_PENALTY=0.411756
PRIMER_RIGHT_4_PENALTY=5.019637
PRIMER_LEFT_4_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_4_SEQUENCE=CTTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_4=929,20
PRIMER_RIGHT_4=1591,23
PRIMER_LEFT_4_TM=60.412
PRIMER_RIGHT_4_TM=57.980
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=663
PRIMER_PAIR_5_PENALTY=5.435969
PRIMER_LEFT_5_PENALTY=0.411756
PRIMER_RIGHT_5_PENALTY=5.024214
PRIMER_LEFT_5_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_5_SEQUENCE=TGACATCCCTCTCTAACCTTTT
PRIMER_LEFT_5=929,20
PRIMER_RIGHT_5=1587,22
PRIMER_LEFT_5_TM=60.412
PRIMER_RIGHT_5_TM=56.976
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=659
PRIMER_PAIR_6_PENALTY=5.435969
PRIMER_LEFT_6_PENALTY=0.411756
PRIMER_RIGHT_6_PENALTY=5.024214
PRIMER_LEFT_6_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_6_SEQUENCE=TTGACATCCCTCTCTAACCTTT
PRIMER_LEFT_6=929,20
PRIMER_RIGHT_6=1588,22
PRIMER_LEFT_6_TM=60.412
PRIMER_RIGHT_6_TM=56.976
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=660
PRIMER_PAIR_7_PENALTY=5.435969
PRIMER_LEFT_7_PENALTY=0.411756
PRIMER_RIGHT_7_PENALTY=5.024214
PRIMER_LEFT_7_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_7_SEQUENCE=TTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_7=929,20
PRIMER_RIGHT_7=1590,22
PRIMER_LEFT_7_TM=60.412
PRIMER_RIGHT_7_TM=56.976
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=662
PRIMER_PAIR_8_PENALTY=5.435969
PRIMER_LEFT_8_PENALTY=0.411756
PRIMER_RIGHT_8_PENALTY=5.024214
PRIMER_LEFT_8_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_8_SEQUENCE=CTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_8=929,20
PRIMER_RIGHT_8=1591,22
PRIMER_LEFT_8_TM=60.412
PRIMER_RIGHT_8_TM=56.976
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=663
PRIMER_PAIR_9_PENALTY=5.535446
PRIMER_LEFT_9_PENALTY=0.411756
PRIMER_RIGHT_9_PENALTY=5.123691
PRIMER_LEFT_9_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_9_SEQUENCE=TTTTGACATCCCTCTCTAACC
PRIMER_LEFT_9=929,20
PRIMER_RIGHT_9=1590,21
PRIMER_LEFT_9_TM=60.412
PRIMER_RIGHT_9_TM=55.876
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=662
PRIMER_PAIR_10_PENALTY=5.544676
PRIMER_LEFT_10_PENALTY=1.153931
PRIMER_RIGHT_10_PENALTY=4.390745
PRIMER_LEFT_10_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_10_SEQUENCE=CACTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_10=888,20
PRIMER_RIGHT_10=1593,24
PRIMER_LEFT_10_TM=58.846
PRIMER_RIGHT_10_TM=60.391
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.833
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.9000
PRIMER_RIGHT_10_END_STABILITY=7.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=706
PRIMER_PAIR_11_PENALTY=5.549418
PRIMER_LEFT_11_PENALTY=0.411756
PRIMER_RIGHT_11_PENALTY=5.137662
PRIMER_LEFT_11_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_11_SEQUENCE=ATCACTTTTGACATCCCTCTCT
PRIMER_LEFT_11=929,20
PRIMER_RIGHT_11=1595,22
PRIMER_LEFT_11_TM=60.412
PRIMER_RIGHT_11_TM=56.862
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=667
PRIMER_PAIR_12_PENALTY=5.568195
PRIMER_LEFT_12_PENALTY=0.411756
PRIMER_RIGHT_12_PENALTY=5.156439
PRIMER_LEFT_12_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_12_SEQUENCE=ACTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_12=929,20
PRIMER_RIGHT_12=1592,23
PRIMER_LEFT_12_TM=60.412
PRIMER_RIGHT_12_TM=57.844
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=664
PRIMER_PAIR_13_PENALTY=5.584379
PRIMER_LEFT_13_PENALTY=0.411756
PRIMER_RIGHT_13_PENALTY=5.172624
PRIMER_LEFT_13_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_13_SEQUENCE=TCACTTTTGACATCCCTCTCTAAC
PRIMER_LEFT_13=929,20
PRIMER_RIGHT_13=1594,24
PRIMER_LEFT_13_TM=60.412
PRIMER_RIGHT_13_TM=58.827
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=41.667
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=5.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=666
PRIMER_PAIR_14_PENALTY=5.634582
PRIMER_LEFT_14_PENALTY=0.411756
PRIMER_RIGHT_14_PENALTY=5.222826
PRIMER_LEFT_14_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_14_SEQUENCE=ACTTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_14=929,20
PRIMER_RIGHT_14=1592,24
PRIMER_LEFT_14_TM=60.412
PRIMER_RIGHT_14_TM=58.777
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=41.667
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=664
PRIMER_PAIR_15_PENALTY=5.699161
PRIMER_LEFT_15_PENALTY=0.411756
PRIMER_RIGHT_15_PENALTY=5.287406
PRIMER_LEFT_15_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_15_SEQUENCE=ATCACTTTTGACATCCCTCTC
PRIMER_LEFT_15=929,20
PRIMER_RIGHT_15=1595,21
PRIMER_LEFT_15_TM=60.412
PRIMER_RIGHT_15_TM=55.713
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=667
PRIMER_PAIR_16_PENALTY=5.759468
PRIMER_LEFT_16_PENALTY=1.153931
PRIMER_RIGHT_16_PENALTY=4.605536
PRIMER_LEFT_16_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_16_SEQUENCE=TCACTTTTGACATCCCTCTCT
PRIMER_LEFT_16=888,20
PRIMER_RIGHT_16=1594,21
PRIMER_LEFT_16_TM=58.846
PRIMER_RIGHT_16_TM=56.394
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.9000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=707
PRIMER_PAIR_17_PENALTY=5.786800
PRIMER_LEFT_17_PENALTY=0.411756
PRIMER_RIGHT_17_PENALTY=5.375044
PRIMER_LEFT_17_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_17_SEQUENCE=CTTTTGACATCCCTCTCTAACCTTT
PRIMER_LEFT_17=929,20
PRIMER_RIGHT_17=1591,25
PRIMER_LEFT_17_TM=60.412
PRIMER_RIGHT_17_TM=60.375
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=663
PRIMER_PAIR_18_PENALTY=5.836389
PRIMER_LEFT_18_PENALTY=0.411756
PRIMER_RIGHT_18_PENALTY=5.424634
PRIMER_LEFT_18_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_18_SEQUENCE=CTTAATCACTTTTGACATCCCTCTC
PRIMER_LEFT_18=929,20
PRIMER_RIGHT_18=1599,25
PRIMER_LEFT_18_TM=60.412
PRIMER_RIGHT_18_TM=59.575
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=671
PRIMER_PAIR_19_PENALTY=5.836389
PRIMER_LEFT_19_PENALTY=0.411756
PRIMER_RIGHT_19_PENALTY=5.424634
PRIMER_LEFT_19_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_19_SEQUENCE=CTCTTAATCACTTTTGACATCCCTC
PRIMER_LEFT_19=929,20
PRIMER_RIGHT_19=1601,25
PRIMER_LEFT_19_TM=60.412
PRIMER_RIGHT_19_TM=59.575
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=673
PRIMER_PAIR_20_PENALTY=5.900569
PRIMER_LEFT_20_PENALTY=1.153931
PRIMER_RIGHT_20_PENALTY=4.746638
PRIMER_LEFT_20_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_20_SEQUENCE=AATCACTTTTGACATCCCTCTC
PRIMER_LEFT_20=888,20
PRIMER_RIGHT_20=1596,22
PRIMER_LEFT_20_TM=58.846
PRIMER_RIGHT_20_TM=57.253
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.9000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=709
PRIMER_PAIR_21_PENALTY=5.924816
PRIMER_LEFT_21_PENALTY=1.534071
PRIMER_RIGHT_21_PENALTY=4.390745
PRIMER_LEFT_21_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_21_SEQUENCE=CACTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_21=928,20
PRIMER_RIGHT_21=1593,24
PRIMER_LEFT_21_TM=58.466
PRIMER_RIGHT_21_TM=60.391
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=45.833
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=7.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=666
PRIMER_PAIR_22_PENALTY=5.942633
PRIMER_LEFT_22_PENALTY=0.411756
PRIMER_RIGHT_22_PENALTY=5.530877
PRIMER_LEFT_22_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_22_SEQUENCE=TGACATCCCTCTCTAACCTTT
PRIMER_LEFT_22=929,20
PRIMER_RIGHT_22=1587,21
PRIMER_LEFT_22_TM=60.412
PRIMER_RIGHT_22_TM=55.469
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=659
PRIMER_PAIR_23_PENALTY=5.942633
PRIMER_LEFT_23_PENALTY=0.411756
PRIMER_RIGHT_23_PENALTY=5.530877
PRIMER_LEFT_23_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_23_SEQUENCE=TTTGACATCCCTCTCTAACCT
PRIMER_LEFT_23=929,20
PRIMER_RIGHT_23=1589,21
PRIMER_LEFT_23_TM=60.412
PRIMER_RIGHT_23_TM=55.469
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=661
PRIMER_PAIR_24_PENALTY=6.002410
PRIMER_LEFT_24_PENALTY=1.153931
PRIMER_RIGHT_24_PENALTY=4.848479
PRIMER_LEFT_24_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_24_SEQUENCE=TCACTTTTGACATCCCTCTC
PRIMER_LEFT_24=888,20
PRIMER_RIGHT_24=1594,20
PRIMER_LEFT_24_TM=58.846
PRIMER_RIGHT_24_TM=55.152
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.9000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=707
PRIMER_PAIR_25_PENALTY=6.139607
PRIMER_LEFT_25_PENALTY=1.534071
PRIMER_RIGHT_25_PENALTY=4.605536
PRIMER_LEFT_25_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_25_SEQUENCE=TCACTTTTGACATCCCTCTCT
PRIMER_LEFT_25=928,20
PRIMER_RIGHT_25=1594,21
PRIMER_LEFT_25_TM=58.466
PRIMER_RIGHT_25_TM=56.394
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=667
PRIMER_PAIR_26_PENALTY=6.173569
PRIMER_LEFT_26_PENALTY=1.153931
PRIMER_RIGHT_26_PENALTY=5.019637
PRIMER_LEFT_26_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_26_SEQUENCE=CTTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_26=888,20
PRIMER_RIGHT_26=1591,23
PRIMER_LEFT_26_TM=58.846
PRIMER_RIGHT_26_TM=57.980
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.9000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=704
PRIMER_PAIR_27_PENALTY=6.178145
PRIMER_LEFT_27_PENALTY=1.153931
PRIMER_RIGHT_27_PENALTY=5.024214
PRIMER_LEFT_27_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_27_SEQUENCE=TGACATCCCTCTCTAACCTTTT
PRIMER_LEFT_27=888,20
PRIMER_RIGHT_27=1587,22
PRIMER_LEFT_27_TM=58.846
PRIMER_RIGHT_27_TM=56.976
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.9000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=700
PRIMER_PAIR_28_PENALTY=6.178145
PRIMER_LEFT_28_PENALTY=1.153931
PRIMER_RIGHT_28_PENALTY=5.024214
PRIMER_LEFT_28_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_28_SEQUENCE=TTGACATCCCTCTCTAACCTTT
PRIMER_LEFT_28=888,20
PRIMER_RIGHT_28=1588,22
PRIMER_LEFT_28_TM=58.846
PRIMER_RIGHT_28_TM=56.976
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.9000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=701
PRIMER_PAIR_29_PENALTY=6.178145
PRIMER_LEFT_29_PENALTY=1.153931
PRIMER_RIGHT_29_PENALTY=5.024214
PRIMER_LEFT_29_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_29_SEQUENCE=TTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_29=888,20
PRIMER_RIGHT_29=1590,22
PRIMER_LEFT_29_TM=58.846
PRIMER_RIGHT_29_TM=56.976
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.9000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=703
PRIMER_PAIR_30_PENALTY=6.178145
PRIMER_LEFT_30_PENALTY=1.153931
PRIMER_RIGHT_30_PENALTY=5.024214
PRIMER_LEFT_30_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_30_SEQUENCE=CTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_30=888,20
PRIMER_RIGHT_30=1591,22
PRIMER_LEFT_30_TM=58.846
PRIMER_RIGHT_30_TM=56.976
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.9000
PRIMER_RIGHT_30_END_STABILITY=7.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=704
PRIMER_PAIR_31_PENALTY=6.277622
PRIMER_LEFT_31_PENALTY=1.153931
PRIMER_RIGHT_31_PENALTY=5.123691
PRIMER_LEFT_31_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_31_SEQUENCE=TTTTGACATCCCTCTCTAACC
PRIMER_LEFT_31=888,20
PRIMER_RIGHT_31=1590,21
PRIMER_LEFT_31_TM=58.846
PRIMER_RIGHT_31_TM=55.876
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.9000
PRIMER_RIGHT_31_END_STABILITY=7.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=703
PRIMER_PAIR_32_PENALTY=6.280709
PRIMER_LEFT_32_PENALTY=1.534071
PRIMER_RIGHT_32_PENALTY=4.746638
PRIMER_LEFT_32_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_32_SEQUENCE=AATCACTTTTGACATCCCTCTC
PRIMER_LEFT_32=928,20
PRIMER_RIGHT_32=1596,22
PRIMER_LEFT_32_TM=58.466
PRIMER_RIGHT_32_TM=57.253
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=669
PRIMER_PAIR_33_PENALTY=6.286525
PRIMER_LEFT_33_PENALTY=0.411756
PRIMER_RIGHT_33_PENALTY=5.874769
PRIMER_LEFT_33_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_33_SEQUENCE=ATCACTTTTGACATCCCTCTCTAAC
PRIMER_LEFT_33=929,20
PRIMER_RIGHT_33=1595,25
PRIMER_LEFT_33_TM=60.412
PRIMER_RIGHT_33_TM=59.125
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=667
PRIMER_PAIR_34_PENALTY=6.291593
PRIMER_LEFT_34_PENALTY=1.153931
PRIMER_RIGHT_34_PENALTY=5.137662
PRIMER_LEFT_34_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_34_SEQUENCE=ATCACTTTTGACATCCCTCTCT
PRIMER_LEFT_34=888,20
PRIMER_RIGHT_34=1595,22
PRIMER_LEFT_34_TM=58.846
PRIMER_RIGHT_34_TM=56.862
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.9000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=708
PRIMER_PAIR_35_PENALTY=6.310370
PRIMER_LEFT_35_PENALTY=1.153931
PRIMER_RIGHT_35_PENALTY=5.156439
PRIMER_LEFT_35_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_35_SEQUENCE=ACTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_35=888,20
PRIMER_RIGHT_35=1592,23
PRIMER_LEFT_35_TM=58.846
PRIMER_RIGHT_35_TM=57.844
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=43.478
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.9000
PRIMER_RIGHT_35_END_STABILITY=7.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=705
PRIMER_PAIR_36_PENALTY=6.326555
PRIMER_LEFT_36_PENALTY=1.153931
PRIMER_RIGHT_36_PENALTY=5.172624
PRIMER_LEFT_36_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_36_SEQUENCE=TCACTTTTGACATCCCTCTCTAAC
PRIMER_LEFT_36=888,20
PRIMER_RIGHT_36=1594,24
PRIMER_LEFT_36_TM=58.846
PRIMER_RIGHT_36_TM=58.827
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=41.667
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.9000
PRIMER_RIGHT_36_END_STABILITY=5.7000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=707
PRIMER_PAIR_37_PENALTY=6.363249
PRIMER_LEFT_37_PENALTY=0.411756
PRIMER_RIGHT_37_PENALTY=5.951493
PRIMER_LEFT_37_SEQUENCE=CGCATGCTATTGCTTTGTTG
PRIMER_RIGHT_37_SEQUENCE=CACTTTTGACATCCCTCTCTAAC
PRIMER_LEFT_37=929,20
PRIMER_RIGHT_37=1593,23
PRIMER_LEFT_37_TM=60.412
PRIMER_RIGHT_37_TM=57.049
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=43.478
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=5.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=665
PRIMER_PAIR_38_PENALTY=6.376757
PRIMER_LEFT_38_PENALTY=1.153931
PRIMER_RIGHT_38_PENALTY=5.222826
PRIMER_LEFT_38_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_38_SEQUENCE=ACTTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_38=888,20
PRIMER_RIGHT_38=1592,24
PRIMER_LEFT_38_TM=58.846
PRIMER_RIGHT_38_TM=58.777
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=41.667
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.9000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=705
PRIMER_PAIR_39_PENALTY=6.382550
PRIMER_LEFT_39_PENALTY=1.534071
PRIMER_RIGHT_39_PENALTY=4.848479
PRIMER_LEFT_39_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_39_SEQUENCE=TCACTTTTGACATCCCTCTC
PRIMER_LEFT_39=928,20
PRIMER_RIGHT_39=1594,20
PRIMER_LEFT_39_TM=58.466
PRIMER_RIGHT_39_TM=55.152
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=667
PRIMER_PAIR_40_PENALTY=6.441337
PRIMER_LEFT_40_PENALTY=1.153931
PRIMER_RIGHT_40_PENALTY=5.287406
PRIMER_LEFT_40_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_40_SEQUENCE=ATCACTTTTGACATCCCTCTC
PRIMER_LEFT_40=888,20
PRIMER_RIGHT_40=1595,21
PRIMER_LEFT_40_TM=58.846
PRIMER_RIGHT_40_TM=55.713
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.9000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=708
PRIMER_PAIR_41_PENALTY=6.528976
PRIMER_LEFT_41_PENALTY=1.153931
PRIMER_RIGHT_41_PENALTY=5.375044
PRIMER_LEFT_41_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_41_SEQUENCE=CTTTTGACATCCCTCTCTAACCTTT
PRIMER_LEFT_41=888,20
PRIMER_RIGHT_41=1591,25
PRIMER_LEFT_41_TM=58.846
PRIMER_RIGHT_41_TM=60.375
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.9000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=704
PRIMER_PAIR_42_PENALTY=6.553709
PRIMER_LEFT_42_PENALTY=1.534071
PRIMER_RIGHT_42_PENALTY=5.019637
PRIMER_LEFT_42_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_42_SEQUENCE=CTTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_42=928,20
PRIMER_RIGHT_42=1591,23
PRIMER_LEFT_42_TM=58.466
PRIMER_RIGHT_42_TM=57.980
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=664
PRIMER_PAIR_43_PENALTY=6.558285
PRIMER_LEFT_43_PENALTY=1.534071
PRIMER_RIGHT_43_PENALTY=5.024214
PRIMER_LEFT_43_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_43_SEQUENCE=TGACATCCCTCTCTAACCTTTT
PRIMER_LEFT_43=928,20
PRIMER_RIGHT_43=1587,22
PRIMER_LEFT_43_TM=58.466
PRIMER_RIGHT_43_TM=56.976
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.3000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=660
PRIMER_PAIR_44_PENALTY=6.558285
PRIMER_LEFT_44_PENALTY=1.534071
PRIMER_RIGHT_44_PENALTY=5.024214
PRIMER_LEFT_44_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_44_SEQUENCE=TTGACATCCCTCTCTAACCTTT
PRIMER_LEFT_44=928,20
PRIMER_RIGHT_44=1588,22
PRIMER_LEFT_44_TM=58.466
PRIMER_RIGHT_44_TM=56.976
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=661
PRIMER_PAIR_45_PENALTY=6.558285
PRIMER_LEFT_45_PENALTY=1.534071
PRIMER_RIGHT_45_PENALTY=5.024214
PRIMER_LEFT_45_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_45_SEQUENCE=TTTTGACATCCCTCTCTAACCT
PRIMER_LEFT_45=928,20
PRIMER_RIGHT_45=1590,22
PRIMER_LEFT_45_TM=58.466
PRIMER_RIGHT_45_TM=56.976
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=663
PRIMER_PAIR_46_PENALTY=6.558285
PRIMER_LEFT_46_PENALTY=1.534071
PRIMER_RIGHT_46_PENALTY=5.024214
PRIMER_LEFT_46_SEQUENCE=ACGCATGCTATTGCTTTGTT
PRIMER_RIGHT_46_SEQUENCE=CTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_46=928,20
PRIMER_RIGHT_46=1591,22
PRIMER_LEFT_46_TM=58.466
PRIMER_RIGHT_46_TM=56.976
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=664
PRIMER_PAIR_47_PENALTY=6.567927
PRIMER_LEFT_47_PENALTY=2.177182
PRIMER_RIGHT_47_PENALTY=4.390745
PRIMER_LEFT_47_SEQUENCE=CAAATGTACGGTTGGGATTCT
PRIMER_RIGHT_47_SEQUENCE=CACTTTTGACATCCCTCTCTAACC
PRIMER_LEFT_47=897,21
PRIMER_RIGHT_47=1593,24
PRIMER_LEFT_47_TM=58.823
PRIMER_RIGHT_47_TM=60.391
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.833
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=7.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=697
PRIMER_PAIR_48_PENALTY=6.578565
PRIMER_LEFT_48_PENALTY=1.153931
PRIMER_RIGHT_48_PENALTY=5.424634
PRIMER_LEFT_48_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_48_SEQUENCE=CTTAATCACTTTTGACATCCCTCTC
PRIMER_LEFT_48=888,20
PRIMER_RIGHT_48=1599,25
PRIMER_LEFT_48_TM=58.846
PRIMER_RIGHT_48_TM=59.575
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.9000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=712
PRIMER_PAIR_49_PENALTY=6.578565
PRIMER_LEFT_49_PENALTY=1.153931
PRIMER_RIGHT_49_PENALTY=5.424634
PRIMER_LEFT_49_SEQUENCE=GCATCAATCCAAATGTACGG
PRIMER_RIGHT_49_SEQUENCE=CTCTTAATCACTTTTGACATCCCTC
PRIMER_LEFT_49=888,20
PRIMER_RIGHT_49=1601,25
PRIMER_LEFT_49_TM=58.846
PRIMER_RIGHT_49_TM=59.575
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.9000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=714
=
SEQUENCE_ID=MAGIC.433_5_16276519_16276612_hi-0_sf-2_134481_86490_TRUE
SEQUENCE_TARGET=950,193
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAATGATAATGTGATTCTTTAACTGTCGNTTAGTGTCGACGTNAGNAAAAAAGAAACACTAAAAATNTTCGGTTCATGTCGTTTTCTCNAATTCCTTTATCTCGTGCTTCTCNTCTCAATACGAGGAGNANGANGTGTTGGCCCATGCGTTAAACAGCAGCACAAAAACCGATCACCAAGAAACACACCCGATTTATTTAGCAGCACACGAAATTGAAATAATATGTANATTNAAAANNATACTCTTAATTTATTCAGTTTNANATNTNTTTTTTGGTTNNGGGTTTATAGACGGTTTCTTAACCGTCAAATCTANTGTGTGTGTGACATATTCNNCTCTCTCTCTCTCTCGAATCTTTCGTGTTGTTTGCTGTTCAAAATTGNAATTTTAAATATTAAAAAAANAGAAGANAACTTGTTCTGCTTCTTGTCGTGTCNGTCACAGTTTGAATNTCTCTCTCTCTTTCTCTCTTCCTCATAAAAATCTCTGCTTTACNTTTTCTTTTCAAAGGGTTTCATTTTGATTNTAGGGTTGTTCTAAATGCGACATTTTGACTGATGGGTCTTTGTTGAATTTGAAGAGAGNAANAGAGAAAGAGATNNTTCNAATGGAGCCTCCNACGGGAATCTTGGCTTCTCTNTGGCAATTTATACTCTTCATTCCTTATTTCACTGGTTTACTGCTTCTCGGTGTCATTAAAGGTAACAAATNTCAGACTTGNGTTCGATGATGTTGACATGAGATTGGCTTNCTNGATTTGTGTAGGCCTCCATTAGGGTTGTTTCATTNATTCATCAATTTGCTTCAACAGTTACTNNAATTAGCATTTCTAACCAATTTAGNGATCTTCAATNCCATAAAGTTCCCNTTTTTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_14455323_14455339_tsu-0_no-0_57350_15412_TRUE
SEQUENCE_TARGET=950,116
SEQUENCE_TEMPLATE=AACTNCAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTACNAAAAAAANTNNATATTCTTACGNAAAAATTNCNNNCATTNTTTTCNTCAGAANATTGTGACNATTTNCATACAAAATTNTGACGTATTTATTTGGNCATAAAAACGTTACTATTTCGTTATAATATTATGACTTAATTGAANGTAACTAATGTTTGTCACTAATTATGTCAAATTTGTTACAAATTATATAGTCATAAATATNANNNNNNNNNNGTNACCAAATATGGTAACTAAATTATACGATAATTGTGACTGAATTTATGTCATGTATAGTTACGAGAATTTTGTAACAACGATGACACAATTTGTCACTNTTTTAGTGACTTTTGCGTTTGGTCATAGATTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_2_6064305_6064419_hi-0_zu-0_131209_6421_TRUE
SEQUENCE_TARGET=950,214
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_12659791_12659858_zu-0_ws-0_4173796_390_TRUE
SEQUENCE_TARGET=950,167
SEQUENCE_TEMPLATE=NGATAATTTATATAGTGTATTATAGATAGATTGATATTCTTGTGAATGTATATATANTTTTTGGTAGACTGAAATCTNAAAAGTANTCAATATAAGTAGGTAGTTGGAGATTTAAGTCTTAAAATATAGCGTTCTATTACTATACANTTTGTTAGTTTTAGATCATAACANTTTTNNAAAAATTTAACGTAGGTCTATATTTAGNTTTTGCTATATACATACAAACACGAATTNAAAAAATCGCACTCTAGTTGAAACCTAAATATATTTTGTTTNACAATNATGNCAAGATNTATAGTAGCATCCTCGAAAGAGAAACNNATATATATGGTTTATACCATTCAAACAAATTAAACAATATACCTCATTACACTTTAATANGTANTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTACAAGTCAATTAATAATTAGGANTTATATTCAGAGNCNGNCCTCAATAGAAGCTACACAAGCANGTGTTTGTGGCCTNGAATGTGTTAATATTAAATTTAGANGCCNTTAANTATGNAAACTTCCAATATAACTCTATTNTTAAAGACTCGTTAAAGTGAAAAAGAGGTGGTGGATTCAAATGAAAAGNTCCAATAGCTCTATTGTTNAAGACTCCTTTCTNNGAAAATGAGTTGGTGGGTTCGAATTGGAATTCATACCTCAAAGATATTATGGAGAANTNAATAGATATACANNNNAAAGAGTNATGGACNTATTGTATNTGATATTAATTANNGTTTTATACGAACNAAACTAATTGATGGGTTTCATTCTCAATTTTCTTATATNTCTAATGGATCCATGCGCAAATGGTACAAANGCAAATATTACACATTTANATTTATNTACATGATCTCGGAAATTTTNTAANANGCTAATGTCCAAATTAGNNTCTTNGTCNGCTTNNATNNCGNNACNNNNATTNANANNGNACTNGANNNNAAANCNNNGNNNNTAANNCTNCNTNNNNAANTNNTNNNACNNANNTGNNANANCACNNNANNANNNANNANTNTGTNNNCTTTGTNNTTNTNTNNNTNNNTATTNTNATNTTCNTNNNTTCTANNNNGNTTCTNTNATNAGNNTNTNNNANCNANNNNGANNTNNTANNNNNNNNNNTNNNTTATTACTAANNNNGTNTGAAGAATNNANATTNGNNTATTACATTTACNNNTTNTTNTGNAGAATNCAAATTAGTTCANTACNCNAAATNAAATATGTGGTNTANCCACTACNAAAAAGCGGCNCATGACGTCGGTTNTTNTTGACCNTGACGTCGATACTAAACCGACTTNAACAAAATAGCATCAGTTTGAAAANCNATCGTNATAACTTGTGACGTNAAGTATTTTGACACCANTATAACATTCGNTGTTTATTTNTGTGTCTAATATTTGAATCGGTTTAAGTAANTTTAGCGACTGTTTTTATGTGNCACNAAAATCAAAAACTCATTTCTTGCTTTGAAAGTTTCTTAAGTCNTTTTAGATTATGACGTNATNAACCGACNNNTNNATAGATAAGTGGNTGCACACTAGTACCATCAAGTCACCACGCCAACTAACTAAAAGACTCACCTAAAAANCNTCTTTATCTTTAATGTATTCTCTTTGTTCATTTGGTTTTACAANCAAAAAAANNNAAAAANANAACACTTCATGCGTAAGACATAAGCAAAATGGCCGGTGGAGGATTGGCTCTTGATGTCTCCAGCGCCGGAAACATTGATGCCAAGATCACAGCCGCAGTTGTCATGAGTTGCATTGTGGCTGCCTCATGTGGTCTCATTTTTGGCTATGACATCGGAATCTCAGGTNACAAATTCAAGNACATAAAAGGNTCATTTTANNAAACAAGTATATTAGTTAATTTATCTTTGATTATAATTTAACAAAGGGGGTGTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.815525
PRIMER_LEFT_0_PENALTY=2.573098
PRIMER_RIGHT_0_PENALTY=0.242426
PRIMER_LEFT_0_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_0_SEQUENCE=TTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_0=728,22
PRIMER_RIGHT_0=1725,20
PRIMER_LEFT_0_TM=60.573
PRIMER_RIGHT_0_TM=59.758
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=998
PRIMER_PAIR_1_PENALTY=2.874246
PRIMER_LEFT_1_PENALTY=2.573098
PRIMER_RIGHT_1_PENALTY=0.301147
PRIMER_LEFT_1_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_1_SEQUENCE=TAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_1=728,22
PRIMER_RIGHT_1=1724,20
PRIMER_LEFT_1_TM=60.573
PRIMER_RIGHT_1_TM=60.301
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=997
PRIMER_PAIR_2_PENALTY=3.322246
PRIMER_LEFT_2_PENALTY=3.079819
PRIMER_RIGHT_2_PENALTY=0.242426
PRIMER_LEFT_2_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_2_SEQUENCE=TTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_2=785,20
PRIMER_RIGHT_2=1725,20
PRIMER_LEFT_2_TM=56.920
PRIMER_RIGHT_2_TM=59.758
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=941
PRIMER_PAIR_3_PENALTY=3.380966
PRIMER_LEFT_3_PENALTY=3.079819
PRIMER_RIGHT_3_PENALTY=0.301147
PRIMER_LEFT_3_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_3_SEQUENCE=TAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_3=785,20
PRIMER_RIGHT_3=1724,20
PRIMER_LEFT_3_TM=56.920
PRIMER_RIGHT_3_TM=60.301
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=940
PRIMER_PAIR_4_PENALTY=4.171112
PRIMER_LEFT_4_PENALTY=2.573098
PRIMER_RIGHT_4_PENALTY=1.598013
PRIMER_LEFT_4_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_4_SEQUENCE=GTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_4=728,22
PRIMER_RIGHT_4=1726,21
PRIMER_LEFT_4_TM=60.573
PRIMER_RIGHT_4_TM=60.598
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=999
PRIMER_PAIR_5_PENALTY=4.677833
PRIMER_LEFT_5_PENALTY=3.079819
PRIMER_RIGHT_5_PENALTY=1.598013
PRIMER_LEFT_5_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_5_SEQUENCE=GTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_5=785,20
PRIMER_RIGHT_5=1726,21
PRIMER_LEFT_5_TM=56.920
PRIMER_RIGHT_5_TM=60.598
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=52.381
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=942
PRIMER_PAIR_6_PENALTY=4.790470
PRIMER_LEFT_6_PENALTY=2.573098
PRIMER_RIGHT_6_PENALTY=2.217372
PRIMER_LEFT_6_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_6_SEQUENCE=AGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_6=728,22
PRIMER_RIGHT_6=1727,21
PRIMER_LEFT_6_TM=60.573
PRIMER_RIGHT_6_TM=58.783
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=1000
PRIMER_PAIR_7_PENALTY=4.844081
PRIMER_LEFT_7_PENALTY=2.573098
PRIMER_RIGHT_7_PENALTY=2.270982
PRIMER_LEFT_7_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_7_SEQUENCE=GTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_7=728,22
PRIMER_RIGHT_7=1726,20
PRIMER_LEFT_7_TM=60.573
PRIMER_RIGHT_7_TM=57.729
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=999
PRIMER_PAIR_8_PENALTY=5.127705
PRIMER_LEFT_8_PENALTY=2.573098
PRIMER_RIGHT_8_PENALTY=2.554607
PRIMER_LEFT_8_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_8_SEQUENCE=GTTGGCGTGGTGACTTGATG
PRIMER_LEFT_8=728,22
PRIMER_RIGHT_8=1722,20
PRIMER_LEFT_8_TM=60.573
PRIMER_RIGHT_8_TM=62.555
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=995
PRIMER_PAIR_9_PENALTY=5.211191
PRIMER_LEFT_9_PENALTY=2.573098
PRIMER_RIGHT_9_PENALTY=2.638093
PRIMER_LEFT_9_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_9_SEQUENCE=TTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_9=728,22
PRIMER_RIGHT_9=1725,21
PRIMER_LEFT_9_TM=60.573
PRIMER_RIGHT_9_TM=61.638
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=998
PRIMER_PAIR_10_PENALTY=5.253145
PRIMER_LEFT_10_PENALTY=2.573098
PRIMER_RIGHT_10_PENALTY=2.680047
PRIMER_LEFT_10_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_10_SEQUENCE=AGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_10=728,22
PRIMER_RIGHT_10=1727,20
PRIMER_LEFT_10_TM=60.573
PRIMER_RIGHT_10_TM=57.320
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=1000
PRIMER_PAIR_11_PENALTY=5.297191
PRIMER_LEFT_11_PENALTY=3.079819
PRIMER_RIGHT_11_PENALTY=2.217372
PRIMER_LEFT_11_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_11_SEQUENCE=AGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_11=785,20
PRIMER_RIGHT_11=1727,21
PRIMER_LEFT_11_TM=56.920
PRIMER_RIGHT_11_TM=58.783
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=943
PRIMER_PAIR_12_PENALTY=5.350801
PRIMER_LEFT_12_PENALTY=3.079819
PRIMER_RIGHT_12_PENALTY=2.270982
PRIMER_LEFT_12_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_12_SEQUENCE=GTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_12=785,20
PRIMER_RIGHT_12=1726,20
PRIMER_LEFT_12_TM=56.920
PRIMER_RIGHT_12_TM=57.729
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=942
PRIMER_PAIR_13_PENALTY=5.394878
PRIMER_LEFT_13_PENALTY=3.079819
PRIMER_RIGHT_13_PENALTY=2.315058
PRIMER_LEFT_13_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_13_SEQUENCE=TTTTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_13=785,20
PRIMER_RIGHT_13=1731,22
PRIMER_LEFT_13_TM=56.920
PRIMER_RIGHT_13_TM=59.685
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=947
PRIMER_PAIR_14_PENALTY=5.634426
PRIMER_LEFT_14_PENALTY=3.079819
PRIMER_RIGHT_14_PENALTY=2.554607
PRIMER_LEFT_14_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_14_SEQUENCE=GTTGGCGTGGTGACTTGATG
PRIMER_LEFT_14=785,20
PRIMER_RIGHT_14=1722,20
PRIMER_LEFT_14_TM=56.920
PRIMER_RIGHT_14_TM=62.555
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=938
PRIMER_PAIR_15_PENALTY=5.717912
PRIMER_LEFT_15_PENALTY=3.079819
PRIMER_RIGHT_15_PENALTY=2.638093
PRIMER_LEFT_15_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_15_SEQUENCE=TTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_15=785,20
PRIMER_RIGHT_15=1725,21
PRIMER_LEFT_15_TM=56.920
PRIMER_RIGHT_15_TM=61.638
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=941
PRIMER_PAIR_16_PENALTY=5.721551
PRIMER_LEFT_16_PENALTY=3.079819
PRIMER_RIGHT_16_PENALTY=2.641731
PRIMER_LEFT_16_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_16_SEQUENCE=TTTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_16=785,20
PRIMER_RIGHT_16=1730,21
PRIMER_LEFT_16_TM=56.920
PRIMER_RIGHT_16_TM=58.358
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=946
PRIMER_PAIR_17_PENALTY=5.759866
PRIMER_LEFT_17_PENALTY=3.079819
PRIMER_RIGHT_17_PENALTY=2.680047
PRIMER_LEFT_17_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_17_SEQUENCE=AGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_17=785,20
PRIMER_RIGHT_17=1727,20
PRIMER_LEFT_17_TM=56.920
PRIMER_RIGHT_17_TM=57.320
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=943
PRIMER_PAIR_18_PENALTY=6.064597
PRIMER_LEFT_18_PENALTY=2.573098
PRIMER_RIGHT_18_PENALTY=3.491498
PRIMER_LEFT_18_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_18_SEQUENCE=AGTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_18=728,22
PRIMER_RIGHT_18=1727,22
PRIMER_LEFT_18_TM=60.573
PRIMER_RIGHT_18_TM=61.491
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=1000
PRIMER_PAIR_19_PENALTY=6.174881
PRIMER_LEFT_19_PENALTY=3.079819
PRIMER_RIGHT_19_PENALTY=3.095061
PRIMER_LEFT_19_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_19_SEQUENCE=TTTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_19=785,20
PRIMER_RIGHT_19=1731,21
PRIMER_LEFT_19_TM=56.920
PRIMER_RIGHT_19_TM=57.905
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=947
PRIMER_PAIR_20_PENALTY=6.181823
PRIMER_LEFT_20_PENALTY=3.079819
PRIMER_RIGHT_20_PENALTY=3.102004
PRIMER_LEFT_20_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_20_SEQUENCE=TTAGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_20=785,20
PRIMER_RIGHT_20=1729,23
PRIMER_LEFT_20_TM=56.920
PRIMER_RIGHT_20_TM=60.102
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=43.478
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=945
PRIMER_PAIR_21_PENALTY=6.181823
PRIMER_LEFT_21_PENALTY=3.079819
PRIMER_RIGHT_21_PENALTY=3.102004
PRIMER_LEFT_21_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_21_SEQUENCE=TTTAGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_21=785,20
PRIMER_RIGHT_21=1730,23
PRIMER_LEFT_21_TM=56.920
PRIMER_RIGHT_21_TM=60.102
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=43.478
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=946
PRIMER_PAIR_22_PENALTY=6.198605
PRIMER_LEFT_22_PENALTY=3.079819
PRIMER_RIGHT_22_PENALTY=3.118785
PRIMER_LEFT_22_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_22_SEQUENCE=TTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_22=785,20
PRIMER_RIGHT_22=1729,20
PRIMER_LEFT_22_TM=56.920
PRIMER_RIGHT_22_TM=56.881
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=945
PRIMER_PAIR_23_PENALTY=6.203958
PRIMER_LEFT_23_PENALTY=3.079819
PRIMER_RIGHT_23_PENALTY=3.124138
PRIMER_LEFT_23_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_23_SEQUENCE=CTTTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_23=785,20
PRIMER_RIGHT_23=1732,22
PRIMER_LEFT_23_TM=56.920
PRIMER_RIGHT_23_TM=58.876
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=948
PRIMER_PAIR_24_PENALTY=6.242274
PRIMER_LEFT_24_PENALTY=3.079819
PRIMER_RIGHT_24_PENALTY=3.162454
PRIMER_LEFT_24_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_24_SEQUENCE=TAGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_24=785,20
PRIMER_RIGHT_24=1728,22
PRIMER_LEFT_24_TM=56.920
PRIMER_RIGHT_24_TM=58.838
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=944
PRIMER_PAIR_25_PENALTY=6.242274
PRIMER_LEFT_25_PENALTY=3.079819
PRIMER_RIGHT_25_PENALTY=3.162454
PRIMER_LEFT_25_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_25_SEQUENCE=TTAGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_25=785,20
PRIMER_RIGHT_25=1729,22
PRIMER_LEFT_25_TM=56.920
PRIMER_RIGHT_25_TM=58.838
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=945
PRIMER_PAIR_26_PENALTY=6.571318
PRIMER_LEFT_26_PENALTY=3.079819
PRIMER_RIGHT_26_PENALTY=3.491498
PRIMER_LEFT_26_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_26_SEQUENCE=AGTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_26=785,20
PRIMER_RIGHT_26=1727,22
PRIMER_LEFT_26_TM=56.920
PRIMER_RIGHT_26_TM=61.491
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=943
PRIMER_PAIR_27_PENALTY=6.633844
PRIMER_LEFT_27_PENALTY=3.079819
PRIMER_RIGHT_27_PENALTY=3.554025
PRIMER_LEFT_27_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_27_SEQUENCE=CTTTTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_27=785,20
PRIMER_RIGHT_27=1732,23
PRIMER_LEFT_27_TM=56.920
PRIMER_RIGHT_27_TM=60.554
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=948
PRIMER_PAIR_28_PENALTY=6.633844
PRIMER_LEFT_28_PENALTY=3.079819
PRIMER_RIGHT_28_PENALTY=3.554025
PRIMER_LEFT_28_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_28_SEQUENCE=TCTTTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_28=785,20
PRIMER_RIGHT_28=1733,23
PRIMER_LEFT_28_TM=56.920
PRIMER_RIGHT_28_TM=60.554
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=949
PRIMER_PAIR_29_PENALTY=6.641268
PRIMER_LEFT_29_PENALTY=3.079819
PRIMER_RIGHT_29_PENALTY=3.561448
PRIMER_LEFT_29_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_29_SEQUENCE=TTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_29=785,20
PRIMER_RIGHT_29=1730,20
PRIMER_LEFT_29_TM=56.920
PRIMER_RIGHT_29_TM=56.439
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=946
PRIMER_PAIR_30_PENALTY=6.645443
PRIMER_LEFT_30_PENALTY=3.079819
PRIMER_RIGHT_30_PENALTY=3.565623
PRIMER_LEFT_30_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_30_SEQUENCE=TAGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_30=785,20
PRIMER_RIGHT_30=1728,21
PRIMER_LEFT_30_TM=56.920
PRIMER_RIGHT_30_TM=57.434
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=944
PRIMER_PAIR_31_PENALTY=6.923429
PRIMER_LEFT_31_PENALTY=3.079819
PRIMER_RIGHT_31_PENALTY=3.843610
PRIMER_LEFT_31_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_31_SEQUENCE=TCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_31=785,20
PRIMER_RIGHT_31=1733,21
PRIMER_LEFT_31_TM=56.920
PRIMER_RIGHT_31_TM=57.156
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=949
PRIMER_PAIR_32_PENALTY=6.954178
PRIMER_LEFT_32_PENALTY=2.573098
PRIMER_RIGHT_32_PENALTY=4.381080
PRIMER_LEFT_32_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_32_SEQUENCE=GTTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_32=728,22
PRIMER_RIGHT_32=1726,22
PRIMER_LEFT_32_TM=60.573
PRIMER_RIGHT_32_TM=62.381
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=999
PRIMER_PAIR_33_PENALTY=6.986088
PRIMER_LEFT_33_PENALTY=2.573098
PRIMER_RIGHT_33_PENALTY=4.412989
PRIMER_LEFT_33_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_33_SEQUENCE=AGTTGGCGTGGTGACTTGATG
PRIMER_LEFT_33=728,22
PRIMER_RIGHT_33=1723,21
PRIMER_LEFT_33_TM=60.573
PRIMER_RIGHT_33_TM=63.413
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=996
PRIMER_PAIR_34_PENALTY=7.063802
PRIMER_LEFT_34_PENALTY=3.079819
PRIMER_RIGHT_34_PENALTY=3.983983
PRIMER_LEFT_34_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_34_SEQUENCE=TCTTTTAGTTAGTTGGCGTGGT
PRIMER_LEFT_34=785,20
PRIMER_RIGHT_34=1733,22
PRIMER_LEFT_34_TM=56.920
PRIMER_RIGHT_34_TM=58.016
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=949
PRIMER_PAIR_35_PENALTY=7.063802
PRIMER_LEFT_35_PENALTY=3.079819
PRIMER_RIGHT_35_PENALTY=3.983983
PRIMER_LEFT_35_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_35_SEQUENCE=GTCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_35=785,20
PRIMER_RIGHT_35=1734,22
PRIMER_LEFT_35_TM=56.920
PRIMER_RIGHT_35_TM=58.016
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=950
PRIMER_PAIR_36_PENALTY=7.139041
PRIMER_LEFT_36_PENALTY=3.079819
PRIMER_RIGHT_36_PENALTY=4.059222
PRIMER_LEFT_36_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_36_SEQUENCE=AGTCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_36=785,20
PRIMER_RIGHT_36=1735,23
PRIMER_LEFT_36_TM=56.920
PRIMER_RIGHT_36_TM=58.941
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=9.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=951
PRIMER_PAIR_37_PENALTY=7.197488
PRIMER_LEFT_37_PENALTY=3.079819
PRIMER_RIGHT_37_PENALTY=4.117668
PRIMER_LEFT_37_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_37_SEQUENCE=TGAGTCTTTTAGTTAGTTGGCGTG
PRIMER_LEFT_37=785,20
PRIMER_RIGHT_37=1737,24
PRIMER_LEFT_37_TM=56.920
PRIMER_RIGHT_37_TM=59.882
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=41.667
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=953
PRIMER_PAIR_38_PENALTY=7.220875
PRIMER_LEFT_38_PENALTY=2.318379
PRIMER_RIGHT_38_PENALTY=4.902496
PRIMER_LEFT_38_SEQUENCE=AAGTGAAAAAGAGGTGGTGGA
PRIMER_RIGHT_38_SEQUENCE=TGGTGACTTGATGGTACTAGTGTG
PRIMER_LEFT_38=716,21
PRIMER_RIGHT_38=1715,24
PRIMER_LEFT_38_TM=58.682
PRIMER_RIGHT_38_TM=59.098
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.833
PRIMER_LEFT_38_SELF_ANY=1.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=1000
PRIMER_PAIR_39_PENALTY=7.238822
PRIMER_LEFT_39_PENALTY=6.996395
PRIMER_RIGHT_39_PENALTY=0.242426
PRIMER_LEFT_39_SEQUENCE=GAGGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_39_SEQUENCE=TTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_39=726,24
PRIMER_RIGHT_39=1725,20
PRIMER_LEFT_39_TM=62.996
PRIMER_RIGHT_39_TM=59.758
PRIMER_LEFT_39_GC_PERCENT=41.667
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=1000
PRIMER_PAIR_40_PENALTY=7.264061
PRIMER_LEFT_40_PENALTY=3.079819
PRIMER_RIGHT_40_PENALTY=4.184242
PRIMER_LEFT_40_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_40_SEQUENCE=GTCTTTTAGTTAGTTGGCGTGGT
PRIMER_LEFT_40=785,20
PRIMER_RIGHT_40=1734,23
PRIMER_LEFT_40_TM=56.920
PRIMER_RIGHT_40_TM=58.816
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=43.478
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=950
PRIMER_PAIR_41_PENALTY=7.297543
PRIMER_LEFT_41_PENALTY=6.996395
PRIMER_RIGHT_41_PENALTY=0.301147
PRIMER_LEFT_41_SEQUENCE=GAGGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_41_SEQUENCE=TAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_41=726,24
PRIMER_RIGHT_41=1724,20
PRIMER_LEFT_41_TM=62.996
PRIMER_RIGHT_41_TM=60.301
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=999
PRIMER_PAIR_42_PENALTY=7.402364
PRIMER_LEFT_42_PENALTY=3.079819
PRIMER_RIGHT_42_PENALTY=4.322545
PRIMER_LEFT_42_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_42_SEQUENCE=AGTCTTTTAGTTAGTTGGCGTGGT
PRIMER_LEFT_42=785,20
PRIMER_RIGHT_42=1735,24
PRIMER_LEFT_42_TM=56.920
PRIMER_RIGHT_42_TM=59.677
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=41.667
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=951
PRIMER_PAIR_43_PENALTY=7.460899
PRIMER_LEFT_43_PENALTY=3.079819
PRIMER_RIGHT_43_PENALTY=4.381080
PRIMER_LEFT_43_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_43_SEQUENCE=GTTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_43=785,20
PRIMER_RIGHT_43=1726,22
PRIMER_LEFT_43_TM=56.920
PRIMER_RIGHT_43_TM=62.381
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=942
PRIMER_PAIR_44_PENALTY=7.475595
PRIMER_LEFT_44_PENALTY=2.573098
PRIMER_RIGHT_44_PENALTY=4.902496
PRIMER_LEFT_44_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_44_SEQUENCE=TGGTGACTTGATGGTACTAGTGTG
PRIMER_LEFT_44=728,22
PRIMER_RIGHT_44=1715,24
PRIMER_LEFT_44_TM=60.573
PRIMER_RIGHT_44_TM=59.098
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=45.833
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=988
PRIMER_PAIR_45_PENALTY=7.492238
PRIMER_LEFT_45_PENALTY=2.573098
PRIMER_RIGHT_45_PENALTY=4.919140
PRIMER_LEFT_45_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_45_SEQUENCE=TGGCGTGGTGACTTGATGGT
PRIMER_LEFT_45=728,22
PRIMER_RIGHT_45=1720,20
PRIMER_LEFT_45_TM=60.573
PRIMER_RIGHT_45_TM=64.919
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.3000
PRIMER_RIGHT_45_END_STABILITY=7.8000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=993
PRIMER_PAIR_46_PENALTY=7.492808
PRIMER_LEFT_46_PENALTY=3.079819
PRIMER_RIGHT_46_PENALTY=4.412989
PRIMER_LEFT_46_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_46_SEQUENCE=AGTTGGCGTGGTGACTTGATG
PRIMER_LEFT_46=785,20
PRIMER_RIGHT_46=1723,21
PRIMER_LEFT_46_TM=56.920
PRIMER_RIGHT_46_TM=63.413
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=939
PRIMER_PAIR_47_PENALTY=7.528253
PRIMER_LEFT_47_PENALTY=3.079819
PRIMER_RIGHT_47_PENALTY=4.448434
PRIMER_LEFT_47_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_47_SEQUENCE=TAGTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_47=785,20
PRIMER_RIGHT_47=1728,23
PRIMER_LEFT_47_TM=56.920
PRIMER_RIGHT_47_TM=61.448
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=47.826
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=944
PRIMER_PAIR_48_PENALTY=7.627139
PRIMER_LEFT_48_PENALTY=3.079819
PRIMER_RIGHT_48_PENALTY=4.547320
PRIMER_LEFT_48_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_48_SEQUENCE=GAGTCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_48=785,20
PRIMER_RIGHT_48=1736,24
PRIMER_LEFT_48_TM=56.920
PRIMER_RIGHT_48_TM=60.547
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.833
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=952
PRIMER_PAIR_49_PENALTY=7.669886
PRIMER_LEFT_49_PENALTY=2.573098
PRIMER_RIGHT_49_PENALTY=5.096788
PRIMER_LEFT_49_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_49_SEQUENCE=GTGGTGACTTGATGGTACTAGTGTG
PRIMER_LEFT_49=728,22
PRIMER_RIGHT_49=1716,25
PRIMER_LEFT_49_TM=60.573
PRIMER_RIGHT_49_TM=59.903
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=48.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=989
=
SEQUENCE_ID=MAGIC.105_4_9035908_9035983_po-0_hi-0_5685_21830_TRUE
SEQUENCE_TARGET=950,175
SEQUENCE_TEMPLATE=AACANCAGCTCTTTTAANTTTTACTTNCTGCGCTAGTTTTTTGTTCCTATTAAAAGAGTNATTCAAGATTTNTTNGGTAAATTCTTAAATGAACNAAAAANAAATTTTGTTAAGNAATCAGAATCAAAGGAAAACNATGGTATAACAGTCTGGTATCNNANNNNANTTGGTNGCTACTCGCTAGTAAACTTCAACCACAACCCTCTGTCCCTCNNTGACGTTGATGCCACTTTTAAGACTAACAACANTANTATTGTCTTTTTNTTCTTCTTTCNAATCCACNTCTNTTGAAATTTTGTTAAATAANNTAAAANATNTNATCTATATGTCNGTGTGTANATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACGGATCTACGAGAAAAATTTAGTGAAGTCGCGGTCGAAGGACCTTAGGTTGGTGGCTAGACCATTTCGTTCAAATCAATCCCACTTTCTTTCATCAAGATAACAAAGATTCACAAACATCCTTACCAAATGATACAACCAAATGACCAACCAAATGATAAAAACTACCTAAGGTCTATCATCAAGTTTTTNTAAGTTCTATTNATCACTTAATATCACNATGGTGCATTCGAGAGGATACAACCAAATGACCAATACACATGTNTTTTTAACTGCTTGTAANTATCACATNTCNCATGATTTTNAAACAAAAATATTGATTTACTTNNTTATTATTATTNTTNTNTTTTCTATACTTATTTGGTTTGATAGTAGANGTTTTTNTATATATTTAAGATATCATTTNNTATATCTTNNNNNNNNNTTTANACCTCTCTTCTTTATTCTACAANCTACCTNTCAAAATNGTTGGTTTTGAANTNTAACATTGGTGATGATTTTTCTTTTGTNTTTAAATGTAACTTTGGATGACAANATTCANTAATAATAATGGGCCTGAGNTTATGAATATAGAATTCCACNTTATCACATTGGACCCAAATTTGCCATGATATAATATAAAACGACGTNGTATCAAGTGTTCAGTGAGTGTGAGTCCTCCGACACGAATAATCTAGAAAATTTTACTAAGACTAGTGTCAGTATTTTTCTACCACACTCGTCNCCTTGCTTTANGCTTTATTCTCTNTTCTTTAGCTTTTTATTTCTTCAGCAAATCTGATTTNAAANAAANTTCATCGANGAAACTGNNAAAAAAAAATGAGTGACGGGAGGAGGAAGAAGAGCGTGAACGGAGGTGCACCGGCGCAAACAATCTTGGATGATCGGAGATCTAGTCTTCCGGAAGTTGAAGCTTCTCCACCGGCTGGGAAACGAGCTGTTATCAAGAGTGCCGATATGAAAGATGATATGCAAAAGGAAGCTATCGAAATCGCCATCTCCGTTAGTCTCTCTTACTCTCTCTTCGTNNTTTATCCNATTTTGTTGTGTACATTTTTCGGATGTANGGATTTGTTATCCTGATCTTGAGTCTTAGGGTATGAGTGATACTAACTGTTCCCTGGGATTTGTTTTTGGAAGTACTCACTTAGTTATCTCTGNAGTTCGATTTCAACNTAGTGTTGTTCTCTCNAAGTCTCAATTTGTTGATCTGAAGGTTATTGTGTACGGAACTAGATTTGATTTCNTGTAACCTTTTNNNNNATTAAACGGTGTTTTTGAATTGCCTAATCAATTGGTTCAACTAANAGTCTGGTGAATTGTCTATGGAGTGATTATATGTGTANTNTGATANATAAGGATTTGCATTCTGAGATTTGCTTTTGCAGGCGTTTGAGAAGTACAGTGTGGAGAAGGATATAGCTGAGAATATAAAGAAGGAGTTTGACAAGAAACATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.861710
PRIMER_LEFT_0_PENALTY=0.727969
PRIMER_RIGHT_0_PENALTY=0.133741
PRIMER_LEFT_0_SEQUENCE=TGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_0_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_0=670,20
PRIMER_RIGHT_0=1469,20
PRIMER_LEFT_0_TM=59.272
PRIMER_RIGHT_0_TM=60.134
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=800
PRIMER_PAIR_1_PENALTY=0.861710
PRIMER_LEFT_1_PENALTY=0.727969
PRIMER_RIGHT_1_PENALTY=0.133741
PRIMER_LEFT_1_SEQUENCE=TGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_1_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_1=670,20
PRIMER_RIGHT_1=1472,20
PRIMER_LEFT_1_TM=59.272
PRIMER_RIGHT_1_TM=60.134
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=803
PRIMER_PAIR_2_PENALTY=0.866026
PRIMER_LEFT_2_PENALTY=0.732286
PRIMER_RIGHT_2_PENALTY=0.133741
PRIMER_LEFT_2_SEQUENCE=CAACCAAATGACCAACCAAA
PRIMER_RIGHT_2_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_2=752,20
PRIMER_RIGHT_2=1469,20
PRIMER_LEFT_2_TM=59.268
PRIMER_RIGHT_2_TM=60.134
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=718
PRIMER_PAIR_3_PENALTY=0.866026
PRIMER_LEFT_3_PENALTY=0.732286
PRIMER_RIGHT_3_PENALTY=0.133741
PRIMER_LEFT_3_SEQUENCE=CAACCAAATGACCAACCAAA
PRIMER_RIGHT_3_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_3=752,20
PRIMER_RIGHT_3=1472,20
PRIMER_LEFT_3_TM=59.268
PRIMER_RIGHT_3_TM=60.134
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=721
PRIMER_PAIR_4_PENALTY=0.912007
PRIMER_LEFT_4_PENALTY=0.778266
PRIMER_RIGHT_4_PENALTY=0.133741
PRIMER_LEFT_4_SEQUENCE=CCAAATGACCAACCAAATGA
PRIMER_RIGHT_4_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_4=755,20
PRIMER_RIGHT_4=1469,20
PRIMER_LEFT_4_TM=59.222
PRIMER_RIGHT_4_TM=60.134
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=715
PRIMER_PAIR_5_PENALTY=0.912007
PRIMER_LEFT_5_PENALTY=0.778266
PRIMER_RIGHT_5_PENALTY=0.133741
PRIMER_LEFT_5_SEQUENCE=CCAAATGACCAACCAAATGA
PRIMER_RIGHT_5_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_5=755,20
PRIMER_RIGHT_5=1472,20
PRIMER_LEFT_5_TM=59.222
PRIMER_RIGHT_5_TM=60.134
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=718
PRIMER_PAIR_6_PENALTY=1.048873
PRIMER_LEFT_6_PENALTY=0.727969
PRIMER_RIGHT_6_PENALTY=0.320904
PRIMER_LEFT_6_SEQUENCE=TGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_6_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_6=670,20
PRIMER_RIGHT_6=1466,20
PRIMER_LEFT_6_TM=59.272
PRIMER_RIGHT_6_TM=59.679
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=797
PRIMER_PAIR_7_PENALTY=1.053190
PRIMER_LEFT_7_PENALTY=0.732286
PRIMER_RIGHT_7_PENALTY=0.320904
PRIMER_LEFT_7_SEQUENCE=CAACCAAATGACCAACCAAA
PRIMER_RIGHT_7_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_7=752,20
PRIMER_RIGHT_7=1466,20
PRIMER_LEFT_7_TM=59.268
PRIMER_RIGHT_7_TM=59.679
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.8000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=715
PRIMER_PAIR_8_PENALTY=1.055572
PRIMER_LEFT_8_PENALTY=0.727969
PRIMER_RIGHT_8_PENALTY=0.327603
PRIMER_LEFT_8_SEQUENCE=TGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_8_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_8=670,20
PRIMER_RIGHT_8=1461,20
PRIMER_LEFT_8_TM=59.272
PRIMER_RIGHT_8_TM=60.328
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=60.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=792
PRIMER_PAIR_9_PENALTY=1.059888
PRIMER_LEFT_9_PENALTY=0.732286
PRIMER_RIGHT_9_PENALTY=0.327603
PRIMER_LEFT_9_SEQUENCE=CAACCAAATGACCAACCAAA
PRIMER_RIGHT_9_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_9=752,20
PRIMER_RIGHT_9=1461,20
PRIMER_LEFT_9_TM=59.268
PRIMER_RIGHT_9_TM=60.328
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=60.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.8000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=710
PRIMER_PAIR_10_PENALTY=1.078203
PRIMER_LEFT_10_PENALTY=0.944463
PRIMER_RIGHT_10_PENALTY=0.133741
PRIMER_LEFT_10_SEQUENCE=GGTGGCTAGACCATTTCGTT
PRIMER_RIGHT_10_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_10=668,20
PRIMER_RIGHT_10=1469,20
PRIMER_LEFT_10_TM=59.056
PRIMER_RIGHT_10_TM=60.134
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=802
PRIMER_PAIR_11_PENALTY=1.078203
PRIMER_LEFT_11_PENALTY=0.944463
PRIMER_RIGHT_11_PENALTY=0.133741
PRIMER_LEFT_11_SEQUENCE=GGTGGCTAGACCATTTCGTT
PRIMER_RIGHT_11_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_11=668,20
PRIMER_RIGHT_11=1472,20
PRIMER_LEFT_11_TM=59.056
PRIMER_RIGHT_11_TM=60.134
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=805
PRIMER_PAIR_12_PENALTY=1.099170
PRIMER_LEFT_12_PENALTY=0.778266
PRIMER_RIGHT_12_PENALTY=0.320904
PRIMER_LEFT_12_SEQUENCE=CCAAATGACCAACCAAATGA
PRIMER_RIGHT_12_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_12=755,20
PRIMER_RIGHT_12=1466,20
PRIMER_LEFT_12_TM=59.222
PRIMER_RIGHT_12_TM=59.679
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=712
PRIMER_PAIR_13_PENALTY=1.105869
PRIMER_LEFT_13_PENALTY=0.778266
PRIMER_RIGHT_13_PENALTY=0.327603
PRIMER_LEFT_13_SEQUENCE=CCAAATGACCAACCAAATGA
PRIMER_RIGHT_13_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_13=755,20
PRIMER_RIGHT_13=1461,20
PRIMER_LEFT_13_TM=59.222
PRIMER_RIGHT_13_TM=60.328
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=60.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=707
PRIMER_PAIR_14_PENALTY=1.182188
PRIMER_LEFT_14_PENALTY=0.727969
PRIMER_RIGHT_14_PENALTY=0.454219
PRIMER_LEFT_14_SEQUENCE=TGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_14_SEQUENCE=TGTCGGAGGACTCACACTCA
PRIMER_LEFT_14=670,20
PRIMER_RIGHT_14=1281,20
PRIMER_LEFT_14_TM=59.272
PRIMER_RIGHT_14_TM=60.454
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=612
PRIMER_PAIR_15_PENALTY=1.186504
PRIMER_LEFT_15_PENALTY=0.732286
PRIMER_RIGHT_15_PENALTY=0.454219
PRIMER_LEFT_15_SEQUENCE=CAACCAAATGACCAACCAAA
PRIMER_RIGHT_15_SEQUENCE=TGTCGGAGGACTCACACTCA
PRIMER_LEFT_15=752,20
PRIMER_RIGHT_15=1281,20
PRIMER_LEFT_15_TM=59.268
PRIMER_RIGHT_15_TM=60.454
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.8000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=530
PRIMER_PAIR_16_PENALTY=1.201664
PRIMER_LEFT_16_PENALTY=0.727969
PRIMER_RIGHT_16_PENALTY=0.473695
PRIMER_LEFT_16_SEQUENCE=TGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_16_SEQUENCE=TCCTCCCGTCACTCATTTTT
PRIMER_LEFT_16=670,20
PRIMER_RIGHT_16=1451,20
PRIMER_LEFT_16_TM=59.272
PRIMER_RIGHT_16_TM=59.526
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=782
PRIMER_PAIR_17_PENALTY=1.205980
PRIMER_LEFT_17_PENALTY=0.732286
PRIMER_RIGHT_17_PENALTY=0.473695
PRIMER_LEFT_17_SEQUENCE=CAACCAAATGACCAACCAAA
PRIMER_RIGHT_17_SEQUENCE=TCCTCCCGTCACTCATTTTT
PRIMER_LEFT_17=752,20
PRIMER_RIGHT_17=1451,20
PRIMER_LEFT_17_TM=59.268
PRIMER_RIGHT_17_TM=59.526
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.8000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=700
PRIMER_PAIR_18_PENALTY=1.222131
PRIMER_LEFT_18_PENALTY=1.088390
PRIMER_RIGHT_18_PENALTY=0.133741
PRIMER_LEFT_18_SEQUENCE=GCATTCGAGAGGATACAACCA
PRIMER_RIGHT_18_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_18=842,21
PRIMER_RIGHT_18=1469,20
PRIMER_LEFT_18_TM=60.088
PRIMER_RIGHT_18_TM=60.134
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=628
PRIMER_PAIR_19_PENALTY=1.222131
PRIMER_LEFT_19_PENALTY=1.088390
PRIMER_RIGHT_19_PENALTY=0.133741
PRIMER_LEFT_19_SEQUENCE=GCATTCGAGAGGATACAACCA
PRIMER_RIGHT_19_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_19=842,21
PRIMER_RIGHT_19=1472,20
PRIMER_LEFT_19_TM=60.088
PRIMER_RIGHT_19_TM=60.134
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=631
PRIMER_PAIR_20_PENALTY=1.222131
PRIMER_LEFT_20_PENALTY=1.088390
PRIMER_RIGHT_20_PENALTY=0.133741
PRIMER_LEFT_20_SEQUENCE=GGTGCATTCGAGAGGATACAA
PRIMER_RIGHT_20_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_20=839,21
PRIMER_RIGHT_20=1472,20
PRIMER_LEFT_20_TM=60.088
PRIMER_RIGHT_20_TM=60.134
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.0000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=634
PRIMER_PAIR_21_PENALTY=1.222210
PRIMER_LEFT_21_PENALTY=1.088469
PRIMER_RIGHT_21_PENALTY=0.133741
PRIMER_LEFT_21_SEQUENCE=GGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_21_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_21=664,20
PRIMER_RIGHT_21=1469,20
PRIMER_LEFT_21_TM=58.912
PRIMER_RIGHT_21_TM=60.134
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.2000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=806
PRIMER_PAIR_22_PENALTY=1.222210
PRIMER_LEFT_22_PENALTY=1.088469
PRIMER_RIGHT_22_PENALTY=0.133741
PRIMER_LEFT_22_SEQUENCE=GGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_22_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_22=664,20
PRIMER_RIGHT_22=1472,20
PRIMER_LEFT_22_TM=58.912
PRIMER_RIGHT_22_TM=60.134
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=809
PRIMER_PAIR_23_PENALTY=1.232485
PRIMER_LEFT_23_PENALTY=0.778266
PRIMER_RIGHT_23_PENALTY=0.454219
PRIMER_LEFT_23_SEQUENCE=CCAAATGACCAACCAAATGA
PRIMER_RIGHT_23_SEQUENCE=TGTCGGAGGACTCACACTCA
PRIMER_LEFT_23=755,20
PRIMER_RIGHT_23=1281,20
PRIMER_LEFT_23_TM=59.222
PRIMER_RIGHT_23_TM=60.454
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=527
PRIMER_PAIR_24_PENALTY=1.255861
PRIMER_LEFT_24_PENALTY=1.122120
PRIMER_RIGHT_24_PENALTY=0.133741
PRIMER_LEFT_24_SEQUENCE=AGGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_24_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_24=663,21
PRIMER_RIGHT_24=1469,20
PRIMER_LEFT_24_TM=59.878
PRIMER_RIGHT_24_TM=60.134
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.2000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=807
PRIMER_PAIR_25_PENALTY=1.255861
PRIMER_LEFT_25_PENALTY=1.122120
PRIMER_RIGHT_25_PENALTY=0.133741
PRIMER_LEFT_25_SEQUENCE=AGGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_25_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_25=663,21
PRIMER_RIGHT_25=1472,20
PRIMER_LEFT_25_TM=59.878
PRIMER_RIGHT_25_TM=60.134
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.2000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=810
PRIMER_PAIR_26_PENALTY=1.258803
PRIMER_LEFT_26_PENALTY=1.125062
PRIMER_RIGHT_26_PENALTY=0.133741
PRIMER_LEFT_26_SEQUENCE=GTGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_26_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_26=669,21
PRIMER_RIGHT_26=1469,20
PRIMER_LEFT_26_TM=60.125
PRIMER_RIGHT_26_TM=60.134
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=801
PRIMER_PAIR_27_PENALTY=1.258803
PRIMER_LEFT_27_PENALTY=1.125062
PRIMER_RIGHT_27_PENALTY=0.133741
PRIMER_LEFT_27_SEQUENCE=GTGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_27_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_27=669,21
PRIMER_RIGHT_27=1472,20
PRIMER_LEFT_27_TM=60.125
PRIMER_RIGHT_27_TM=60.134
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=804
PRIMER_PAIR_28_PENALTY=1.265367
PRIMER_LEFT_28_PENALTY=0.944463
PRIMER_RIGHT_28_PENALTY=0.320904
PRIMER_LEFT_28_SEQUENCE=GGTGGCTAGACCATTTCGTT
PRIMER_RIGHT_28_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_28=668,20
PRIMER_RIGHT_28=1466,20
PRIMER_LEFT_28_TM=59.056
PRIMER_RIGHT_28_TM=59.679
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=799
PRIMER_PAIR_29_PENALTY=1.272065
PRIMER_LEFT_29_PENALTY=0.944463
PRIMER_RIGHT_29_PENALTY=0.327603
PRIMER_LEFT_29_SEQUENCE=GGTGGCTAGACCATTTCGTT
PRIMER_RIGHT_29_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_29=668,20
PRIMER_RIGHT_29=1461,20
PRIMER_LEFT_29_TM=59.056
PRIMER_RIGHT_29_TM=60.328
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=60.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.4000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=794
PRIMER_PAIR_30_PENALTY=1.398681
PRIMER_LEFT_30_PENALTY=0.944463
PRIMER_RIGHT_30_PENALTY=0.454219
PRIMER_LEFT_30_SEQUENCE=GGTGGCTAGACCATTTCGTT
PRIMER_RIGHT_30_SEQUENCE=TGTCGGAGGACTCACACTCA
PRIMER_LEFT_30=668,20
PRIMER_RIGHT_30=1281,20
PRIMER_LEFT_30_TM=59.056
PRIMER_RIGHT_30_TM=60.454
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.4000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=614
PRIMER_PAIR_31_PENALTY=1.409294
PRIMER_LEFT_31_PENALTY=1.088390
PRIMER_RIGHT_31_PENALTY=0.320904
PRIMER_LEFT_31_SEQUENCE=GCATTCGAGAGGATACAACCA
PRIMER_RIGHT_31_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_31=842,21
PRIMER_RIGHT_31=1466,20
PRIMER_LEFT_31_TM=60.088
PRIMER_RIGHT_31_TM=59.679
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=625
PRIMER_PAIR_32_PENALTY=1.409373
PRIMER_LEFT_32_PENALTY=1.088469
PRIMER_RIGHT_32_PENALTY=0.320904
PRIMER_LEFT_32_SEQUENCE=GGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_32_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_32=664,20
PRIMER_RIGHT_32=1466,20
PRIMER_LEFT_32_TM=58.912
PRIMER_RIGHT_32_TM=59.679
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.2000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=803
PRIMER_PAIR_33_PENALTY=1.409701
PRIMER_LEFT_33_PENALTY=0.727969
PRIMER_RIGHT_33_PENALTY=0.681732
PRIMER_LEFT_33_SEQUENCE=TGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_33_SEQUENCE=GGCGATTTCGATAGCTTCCT
PRIMER_LEFT_33=670,20
PRIMER_RIGHT_33=1612,20
PRIMER_LEFT_33_TM=59.272
PRIMER_RIGHT_33_TM=60.682
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=943
PRIMER_PAIR_34_PENALTY=1.411906
PRIMER_LEFT_34_PENALTY=1.278166
PRIMER_RIGHT_34_PENALTY=0.133741
PRIMER_LEFT_34_SEQUENCE=ACAACCAAATGACCAACCAA
PRIMER_RIGHT_34_SEQUENCE=CGTTCACGCTCTTCTTCCTC
PRIMER_LEFT_34=751,20
PRIMER_RIGHT_34=1469,20
PRIMER_LEFT_34_TM=58.722
PRIMER_RIGHT_34_TM=60.134
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=719
PRIMER_PAIR_35_PENALTY=1.411906
PRIMER_LEFT_35_PENALTY=1.278166
PRIMER_RIGHT_35_PENALTY=0.133741
PRIMER_LEFT_35_SEQUENCE=ACAACCAAATGACCAACCAA
PRIMER_RIGHT_35_SEQUENCE=CTCCGTTCACGCTCTTCTTC
PRIMER_LEFT_35=751,20
PRIMER_RIGHT_35=1472,20
PRIMER_LEFT_35_TM=58.722
PRIMER_RIGHT_35_TM=60.134
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=722
PRIMER_PAIR_36_PENALTY=1.414017
PRIMER_LEFT_36_PENALTY=0.732286
PRIMER_RIGHT_36_PENALTY=0.681732
PRIMER_LEFT_36_SEQUENCE=CAACCAAATGACCAACCAAA
PRIMER_RIGHT_36_SEQUENCE=GGCGATTTCGATAGCTTCCT
PRIMER_LEFT_36=752,20
PRIMER_RIGHT_36=1612,20
PRIMER_LEFT_36_TM=59.268
PRIMER_RIGHT_36_TM=60.682
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.8000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=861
PRIMER_PAIR_37_PENALTY=1.415993
PRIMER_LEFT_37_PENALTY=1.088390
PRIMER_RIGHT_37_PENALTY=0.327603
PRIMER_LEFT_37_SEQUENCE=GCATTCGAGAGGATACAACCA
PRIMER_RIGHT_37_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_37=842,21
PRIMER_RIGHT_37=1461,20
PRIMER_LEFT_37_TM=60.088
PRIMER_RIGHT_37_TM=60.328
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=60.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=620
PRIMER_PAIR_38_PENALTY=1.415993
PRIMER_LEFT_38_PENALTY=1.088390
PRIMER_RIGHT_38_PENALTY=0.327603
PRIMER_LEFT_38_SEQUENCE=GGTGCATTCGAGAGGATACAA
PRIMER_RIGHT_38_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_38=839,21
PRIMER_RIGHT_38=1461,20
PRIMER_LEFT_38_TM=60.088
PRIMER_RIGHT_38_TM=60.328
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=60.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.0000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=623
PRIMER_PAIR_39_PENALTY=1.416072
PRIMER_LEFT_39_PENALTY=1.088469
PRIMER_RIGHT_39_PENALTY=0.327603
PRIMER_LEFT_39_SEQUENCE=GGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_39_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_39=664,20
PRIMER_RIGHT_39=1461,20
PRIMER_LEFT_39_TM=58.912
PRIMER_RIGHT_39_TM=60.328
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=60.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=798
PRIMER_PAIR_40_PENALTY=1.418157
PRIMER_LEFT_40_PENALTY=0.944463
PRIMER_RIGHT_40_PENALTY=0.473695
PRIMER_LEFT_40_SEQUENCE=GGTGGCTAGACCATTTCGTT
PRIMER_RIGHT_40_SEQUENCE=TCCTCCCGTCACTCATTTTT
PRIMER_LEFT_40=668,20
PRIMER_RIGHT_40=1451,20
PRIMER_LEFT_40_TM=59.056
PRIMER_RIGHT_40_TM=59.526
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.4000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=784
PRIMER_PAIR_41_PENALTY=1.443024
PRIMER_LEFT_41_PENALTY=1.122120
PRIMER_RIGHT_41_PENALTY=0.320904
PRIMER_LEFT_41_SEQUENCE=AGGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_41_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_41=663,21
PRIMER_RIGHT_41=1466,20
PRIMER_LEFT_41_TM=59.878
PRIMER_RIGHT_41_TM=59.679
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.2000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=804
PRIMER_PAIR_42_PENALTY=1.445966
PRIMER_LEFT_42_PENALTY=1.125062
PRIMER_RIGHT_42_PENALTY=0.320904
PRIMER_LEFT_42_SEQUENCE=GTGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_42_SEQUENCE=TCACGCTCTTCTTCCTCCTC
PRIMER_LEFT_42=669,21
PRIMER_RIGHT_42=1466,20
PRIMER_LEFT_42_TM=60.125
PRIMER_RIGHT_42_TM=59.679
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=798
PRIMER_PAIR_43_PENALTY=1.449723
PRIMER_LEFT_43_PENALTY=1.122120
PRIMER_RIGHT_43_PENALTY=0.327603
PRIMER_LEFT_43_SEQUENCE=AGGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_43_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_43=663,21
PRIMER_RIGHT_43=1461,20
PRIMER_LEFT_43_TM=59.878
PRIMER_RIGHT_43_TM=60.328
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=60.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.2000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=799
PRIMER_PAIR_44_PENALTY=1.452665
PRIMER_LEFT_44_PENALTY=1.125062
PRIMER_RIGHT_44_PENALTY=0.327603
PRIMER_LEFT_44_SEQUENCE=GTGGCTAGACCATTTCGTTCA
PRIMER_RIGHT_44_SEQUENCE=CTCTTCTTCCTCCTCCCGTC
PRIMER_LEFT_44=669,21
PRIMER_RIGHT_44=1461,20
PRIMER_LEFT_44_TM=60.125
PRIMER_RIGHT_44_TM=60.328
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=60.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=9.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=793
PRIMER_PAIR_45_PENALTY=1.459998
PRIMER_LEFT_45_PENALTY=0.778266
PRIMER_RIGHT_45_PENALTY=0.681732
PRIMER_LEFT_45_SEQUENCE=CCAAATGACCAACCAAATGA
PRIMER_RIGHT_45_SEQUENCE=GGCGATTTCGATAGCTTCCT
PRIMER_LEFT_45=755,20
PRIMER_RIGHT_45=1612,20
PRIMER_LEFT_45_TM=59.222
PRIMER_RIGHT_45_TM=60.682
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=858
PRIMER_PAIR_46_PENALTY=1.542609
PRIMER_LEFT_46_PENALTY=1.088390
PRIMER_RIGHT_46_PENALTY=0.454219
PRIMER_LEFT_46_SEQUENCE=GCATTCGAGAGGATACAACCA
PRIMER_RIGHT_46_SEQUENCE=TGTCGGAGGACTCACACTCA
PRIMER_LEFT_46=842,21
PRIMER_RIGHT_46=1281,20
PRIMER_LEFT_46_TM=60.088
PRIMER_RIGHT_46_TM=60.454
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=440
PRIMER_PAIR_47_PENALTY=1.542609
PRIMER_LEFT_47_PENALTY=1.088390
PRIMER_RIGHT_47_PENALTY=0.454219
PRIMER_LEFT_47_SEQUENCE=GGTGCATTCGAGAGGATACAA
PRIMER_RIGHT_47_SEQUENCE=TGTCGGAGGACTCACACTCA
PRIMER_LEFT_47=839,21
PRIMER_RIGHT_47=1281,20
PRIMER_LEFT_47_TM=60.088
PRIMER_RIGHT_47_TM=60.454
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.0000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=443
PRIMER_PAIR_48_PENALTY=1.542688
PRIMER_LEFT_48_PENALTY=1.088469
PRIMER_RIGHT_48_PENALTY=0.454219
PRIMER_LEFT_48_SEQUENCE=GGTTGGTGGCTAGACCATTT
PRIMER_RIGHT_48_SEQUENCE=TGTCGGAGGACTCACACTCA
PRIMER_LEFT_48=664,20
PRIMER_RIGHT_48=1281,20
PRIMER_LEFT_48_TM=58.912
PRIMER_RIGHT_48_TM=60.454
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.2000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=618
PRIMER_PAIR_49_PENALTY=1.562085
PRIMER_LEFT_49_PENALTY=1.088390
PRIMER_RIGHT_49_PENALTY=0.473695
PRIMER_LEFT_49_SEQUENCE=GCATTCGAGAGGATACAACCA
PRIMER_RIGHT_49_SEQUENCE=TCCTCCCGTCACTCATTTTT
PRIMER_LEFT_49=842,21
PRIMER_RIGHT_49=1451,20
PRIMER_LEFT_49_TM=60.088
PRIMER_RIGHT_49_TM=59.526
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=610
=
SEQUENCE_ID=MAGIC.426_1_19392042_19392167_ler-0_oy-0_5751_2358_TRUE
SEQUENCE_TARGET=950,225
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTGGNGTAANTATAAAAATTGCTGTTTTAAAAGANTAAATGAAGTCGNNCANANAAAAAAANCAANACAAAGNCGNCCCGCGATTATTGTTTTCNGGATTGTAAATTCGANTCANTANCTNNCNTTGAATCATTTTACAAGACTCCTACTTNGNTGGTCATTAATATATTATNANGTCATTTTGTAAGATTAAATAAGTCTGCTTTAAAATANNAATAANNTCTTATTTTAAAAGANTACACGAAGTCGTGANAANAAAAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4634827_4634908_can-0_bur-0_18443_8824_TRUE
SEQUENCE_TARGET=950,181
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANGNTTGAAAANTCTGTTGAGGCNNTNGTGGATTCNCCATGAAGTCGGGCTTCGCGAAATCCTNGACCACCAAATGGTCATTACCANCTCTTGCAAACAATGCATCCGAGCTTTTGNGNNGGNGCTCCACGGANGAGGCNNATNNNNNNNNNNNNTTGAAAANGAGANGGTAACCAACTCTAGAAGACATGGTCNNNNNGCCATACNTTTCNAAACAAACNNANGNTTNCNAATTATNCAANCAANNGATTANNACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGNTAACACCTNCTCCGTAGNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTAACNGATCTCGACAAANGTACCTNTNNANCCNNCNTNCNATATGAAGCTTAGATTTTAATGTTAGTAACACGGATGATCGCTAGATGTATCATTGCTTANAAAATTNTCAAAAAAATATANANTTTATAGTAAATTATGCAAACATGGCAAAATCTTTTATCCATAGATTTGAATGTGTTGCAATAATGTTAGTGACTAACTTTGTAATAGTCACAAGAGTTGATACATCAAGAATAAATCAGATAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_1864135_1864680_can-0_ct-1_149208_506718_TRUE
SEQUENCE_TARGET=950,645
SEQUENCE_TEMPLATE=ATCTTTCACCGGTGGTCTCGATTTCCAGTTCTCGCCGGATATATCAGTCTCGAAATAGTCCCCACCAATGCAAACGTTTTTTCCAGATTCGTCTATGGCTTGAAATTTGAATTCATGAATCTCTCCGGCGGTTAACTCCACAGAATCAATCCCGTCGACACCGGGAACTGAAATCTCGACGGTCTTCGCTTCTCTACACGGCGCTCCTCCCGGAGCCAACCAACCTCTCATCAAATTCGCCGTGAAATTCTTCTGAGATCCAGCGGTTATCCACTCGAACTGGACCCGGGTCGAGTTTGAAGTGAGATTCGTCTTCTTCGGAACCAGATTTCGTGGAGCCATCATCAAGGTGAGATTTTGGCGGTGAGGTTTCTCTGATACTAGATTGGGTTTCGTTGACATAAACGGCGACGGAGAAGGACTGATTCGAACAGAGTAAGCATTGAATCTCCATGGCTGAACGAAGCTTTGAATCGAACAACCGTCAATACTCCAAACGACTAACATAAGGAAGCAGACGAGTGCTGTAAGAGCACCAAGCCGCCATTGAAGAAGAGGGCTTGGCCGTAAAATCACCAATTGGCTCGGTACCGGCGGGAAAATCATTCCTTTCTCCGGCATCTGAAGATCCGACAACAAGAAACAAAAACCCAGAAAATGAGGATTCAATAATTTGACGACAAACAAGGCAATTACCGAGAAAAGATTAAAAGTCATAATACTAACTTTATAGTAAGATAATATTAAACTTACTTTAAATGCTTCAATGGCGGATAACTTCTCCAAACTCGTAAGTNNNAGAAGAAGAAGATTAGATGATGACAATGAATGATACACAATCCACAATTGGCANTTTTTTTAATTTTTGGACAAGAAGAACCTTAAAATCCTCCCAGATTTCCAGAAGACAAGGGAGGATTAAAAAGTCTTAGATTTTTCTTGACTAGACAAAACGAAATGTATACTAATAAGTGCAAAGATAAGGAAAAGAACTCAAAATNTGACAGCTAAAATTGATCTCCCAAAGAAAAATTTAGGATTCTGATTTCTCAGTGCAGCCAAAAAAGCAATTTAGAGGAGGCGGACGGAGAGAAAAAGACAATTTTNNGGATTGACGGTTATACCCCTGATGTTTACTGGATTTACCATGACACCAACCCGACGACCGCGCTAAATAATTGCCCAGCGGACATGTGGGCAACGAAGTCGCGTCGGTGTGCCGGTAGGGGTACTGTTGTCTTTTNCTTTAAACAATTAATACGTCGTGTATGGTGTTGATTGGCGTCACATGCATTTGTTTTGTTATTGTNCTCTCTCTCTCCACCGTCGCTCCTATTTTCTCTGCAGAAGTGAAAAGAAAAAAAATTGTTTTGTCAAAAAAATGGCTCAATAAANAAGACGATTGTCACTTTAAAAAGAAAATAGTAATTATCCAAATATCAATTTCAGAGATTTTTTNGGCTAAAAAGATAAAAGAGCTAGCTCAGATGTTATAATTATCAGTAAATGGAAAACCGTTGGAGATTTAGTACAACTAACAATTTTNGATAAGACAATATAANANTTTTNTTCTTCTTAGATTAGTAGTTGTCTATTTTCAATACCACTTTAGCTTAATCAAAGTGTCCATTCACATTATTCANAAAAATAGTTCATCTATTTTATTAATCTTCTTACTTTGATTCAAGAATTCNTTTTTTTTTNGTTGTTGCTCAAGATAGAATTTAGCTTATACATAAAAATGATACTTTTTCGCAGTTTTAAAATTCTCTAAGAAACCAAACTGATAAAATTTAAAGCGCAAGAGAATATTAAAGTAAGAAAAAGAGTAAATTATTACAGAATCTCCTCACTAAACGAGCTTATACGTTAATGGCTCTAATCTTGAATATGTTGTTGTTGGCCGTTGAGGCTGTGTTTAATCATGTGACTTTTAACTCCAAAGCTACCAAAAGTCTTCGGCCCAAGAGAAAAGAGCTACATGAGTACATTCTACATTCTTCAGCCAATAATGAGACTCCCTACTATCTTAANTATTGGGCCGAATATGGGCCTACATTAACTATTTANNNNNNNNNATAGTATTGTTAGCATCTAGCTTTTTATNATTTTGTTTCAGTGAGGAGAAAAACGCTTTCTCAATTATGAACTNTGCATCTTTGTCACTGATTCTTCTCACCTGATCTGTTGCAGATATTTGGTCAAAGAGTTTATTAAAAGATAACATAATATAAAGTGAAAAAGCAATGCTGCTTTCTCTCATTGGTTGTGCATAGAGCAAGGACAGAGAAAAACAGGTTCGTACGTAAGAAACACACATTAACTGTGTTCTGCTTCCTTTGTATTCTCTCACTTTTCTCNTTTTTCAGAATTCAATCTCTGGAAATTTTCTTCCTAAAGANTAAAGAGAACCGTTTGGACCAGATCAGCTTGCCTCTTGGTAATGGTCTCTCTTCTCTTTGTTCCCAATTTCTGTAGCTTTCTTCTTTCTCCCTAACTCGTCTGCTCTCTTTGTCTCTTCCTCTCACAGTTCATACGTCTTCCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.539822
PRIMER_LEFT_0_PENALTY=0.227274
PRIMER_RIGHT_0_PENALTY=4.312548
PRIMER_LEFT_0_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_0_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_0=898,20
PRIMER_RIGHT_0=1861,24
PRIMER_LEFT_0_TM=59.773
PRIMER_RIGHT_0_TM=60.313
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.833
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=964
PRIMER_PAIR_1_PENALTY=4.631072
PRIMER_LEFT_1_PENALTY=0.227274
PRIMER_RIGHT_1_PENALTY=4.403798
PRIMER_LEFT_1_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_1_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_1=898,20
PRIMER_RIGHT_1=1876,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=57.596
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=979
PRIMER_PAIR_2_PENALTY=5.057578
PRIMER_LEFT_2_PENALTY=0.745029
PRIMER_RIGHT_2_PENALTY=4.312548
PRIMER_LEFT_2_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_2_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_2=897,20
PRIMER_RIGHT_2=1861,24
PRIMER_LEFT_2_TM=59.255
PRIMER_RIGHT_2_TM=60.313
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.833
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=965
PRIMER_PAIR_3_PENALTY=5.148828
PRIMER_LEFT_3_PENALTY=0.745029
PRIMER_RIGHT_3_PENALTY=4.403798
PRIMER_LEFT_3_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_3_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_3=897,20
PRIMER_RIGHT_3=1876,22
PRIMER_LEFT_3_TM=59.255
PRIMER_RIGHT_3_TM=57.596
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=980
PRIMER_PAIR_4_PENALTY=5.371865
PRIMER_LEFT_4_PENALTY=1.059316
PRIMER_RIGHT_4_PENALTY=4.312548
PRIMER_LEFT_4_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_4_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_4=899,21
PRIMER_RIGHT_4=1861,24
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=60.313
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=963
PRIMER_PAIR_5_PENALTY=5.398751
PRIMER_LEFT_5_PENALTY=0.227274
PRIMER_RIGHT_5_PENALTY=5.171477
PRIMER_LEFT_5_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_5_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_5=898,20
PRIMER_RIGHT_5=1868,23
PRIMER_LEFT_5_TM=59.773
PRIMER_RIGHT_5_TM=57.829
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=971
PRIMER_PAIR_6_PENALTY=5.447393
PRIMER_LEFT_6_PENALTY=0.227274
PRIMER_RIGHT_6_PENALTY=5.220120
PRIMER_LEFT_6_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_6_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_6=898,20
PRIMER_RIGHT_6=1870,25
PRIMER_LEFT_6_TM=59.773
PRIMER_RIGHT_6_TM=59.780
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=973
PRIMER_PAIR_7_PENALTY=5.463115
PRIMER_LEFT_7_PENALTY=1.059316
PRIMER_RIGHT_7_PENALTY=4.403798
PRIMER_LEFT_7_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_7_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_7=899,21
PRIMER_RIGHT_7=1876,22
PRIMER_LEFT_7_TM=60.059
PRIMER_RIGHT_7_TM=57.596
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=978
PRIMER_PAIR_8_PENALTY=5.487331
PRIMER_LEFT_8_PENALTY=0.227274
PRIMER_RIGHT_8_PENALTY=5.260057
PRIMER_LEFT_8_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_8_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_8=898,20
PRIMER_RIGHT_8=1868,24
PRIMER_LEFT_8_TM=59.773
PRIMER_RIGHT_8_TM=58.740
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.833
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=5.543440
PRIMER_LEFT_9_PENALTY=0.227274
PRIMER_RIGHT_9_PENALTY=5.316167
PRIMER_LEFT_9_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_9_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAGA
PRIMER_LEFT_9=898,20
PRIMER_RIGHT_9=1868,25
PRIMER_LEFT_9_TM=59.773
PRIMER_RIGHT_9_TM=60.316
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=44.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=971
PRIMER_PAIR_10_PENALTY=5.610881
PRIMER_LEFT_10_PENALTY=0.227274
PRIMER_RIGHT_10_PENALTY=5.383607
PRIMER_LEFT_10_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_10_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_10=898,20
PRIMER_RIGHT_10=1869,24
PRIMER_LEFT_10_TM=59.773
PRIMER_RIGHT_10_TM=58.616
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=41.667
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=972
PRIMER_PAIR_11_PENALTY=5.656721
PRIMER_LEFT_11_PENALTY=1.344173
PRIMER_RIGHT_11_PENALTY=4.312548
PRIMER_LEFT_11_SEQUENCE=TCCCAGATTTCCAGAAGACAA
PRIMER_RIGHT_11_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_11=890,21
PRIMER_RIGHT_11=1861,24
PRIMER_LEFT_11_TM=59.656
PRIMER_RIGHT_11_TM=60.313
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=45.833
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=5.747971
PRIMER_LEFT_12_PENALTY=1.344173
PRIMER_RIGHT_12_PENALTY=4.403798
PRIMER_LEFT_12_SEQUENCE=TCCCAGATTTCCAGAAGACAA
PRIMER_RIGHT_12_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_12=890,21
PRIMER_RIGHT_12=1876,22
PRIMER_LEFT_12_TM=59.656
PRIMER_RIGHT_12_TM=57.596
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=987
PRIMER_PAIR_13_PENALTY=5.750340
PRIMER_LEFT_13_PENALTY=1.437791
PRIMER_RIGHT_13_PENALTY=4.312548
PRIMER_LEFT_13_SEQUENCE=ATTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_13_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_13=896,21
PRIMER_RIGHT_13=1861,24
PRIMER_LEFT_13_TM=59.562
PRIMER_RIGHT_13_TM=60.313
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.833
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=966
PRIMER_PAIR_14_PENALTY=5.760239
PRIMER_LEFT_14_PENALTY=0.227274
PRIMER_RIGHT_14_PENALTY=5.532965
PRIMER_LEFT_14_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_14_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_14=898,20
PRIMER_RIGHT_14=1869,25
PRIMER_LEFT_14_TM=59.773
PRIMER_RIGHT_14_TM=59.467
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=44.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=972
PRIMER_PAIR_15_PENALTY=5.841589
PRIMER_LEFT_15_PENALTY=1.437791
PRIMER_RIGHT_15_PENALTY=4.403798
PRIMER_LEFT_15_SEQUENCE=ATTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_15_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_15=896,21
PRIMER_RIGHT_15=1876,22
PRIMER_LEFT_15_TM=59.562
PRIMER_RIGHT_15_TM=57.596
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=981
PRIMER_PAIR_16_PENALTY=5.886003
PRIMER_LEFT_16_PENALTY=1.573455
PRIMER_RIGHT_16_PENALTY=4.312548
PRIMER_LEFT_16_SEQUENCE=AAAATCCTCCCAGATTTCCA
PRIMER_RIGHT_16_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_16=883,20
PRIMER_RIGHT_16=1861,24
PRIMER_LEFT_16_TM=58.427
PRIMER_RIGHT_16_TM=60.313
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.833
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=979
PRIMER_PAIR_17_PENALTY=5.906226
PRIMER_LEFT_17_PENALTY=1.593677
PRIMER_RIGHT_17_PENALTY=4.312548
PRIMER_LEFT_17_SEQUENCE=AAAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_17_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_17=883,21
PRIMER_RIGHT_17=1861,24
PRIMER_LEFT_17_TM=59.406
PRIMER_RIGHT_17_TM=60.313
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.833
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=979
PRIMER_PAIR_18_PENALTY=5.916507
PRIMER_LEFT_18_PENALTY=0.745029
PRIMER_RIGHT_18_PENALTY=5.171477
PRIMER_LEFT_18_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_18_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_18=897,20
PRIMER_RIGHT_18=1868,23
PRIMER_LEFT_18_TM=59.255
PRIMER_RIGHT_18_TM=57.829
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=972
PRIMER_PAIR_19_PENALTY=5.965149
PRIMER_LEFT_19_PENALTY=0.745029
PRIMER_RIGHT_19_PENALTY=5.220120
PRIMER_LEFT_19_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_19_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_19=897,20
PRIMER_RIGHT_19=1870,25
PRIMER_LEFT_19_TM=59.255
PRIMER_RIGHT_19_TM=59.780
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=974
PRIMER_PAIR_20_PENALTY=5.977253
PRIMER_LEFT_20_PENALTY=1.573455
PRIMER_RIGHT_20_PENALTY=4.403798
PRIMER_LEFT_20_SEQUENCE=AAAATCCTCCCAGATTTCCA
PRIMER_RIGHT_20_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_20=883,20
PRIMER_RIGHT_20=1876,22
PRIMER_LEFT_20_TM=58.427
PRIMER_RIGHT_20_TM=57.596
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=994
PRIMER_PAIR_21_PENALTY=5.997475
PRIMER_LEFT_21_PENALTY=1.593677
PRIMER_RIGHT_21_PENALTY=4.403798
PRIMER_LEFT_21_SEQUENCE=AAAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_21_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_21=883,21
PRIMER_RIGHT_21=1876,22
PRIMER_LEFT_21_TM=59.406
PRIMER_RIGHT_21_TM=57.596
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=994
PRIMER_PAIR_22_PENALTY=6.005087
PRIMER_LEFT_22_PENALTY=0.745029
PRIMER_RIGHT_22_PENALTY=5.260057
PRIMER_LEFT_22_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_22_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_22=897,20
PRIMER_RIGHT_22=1868,24
PRIMER_LEFT_22_TM=59.255
PRIMER_RIGHT_22_TM=58.740
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.833
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=972
PRIMER_PAIR_23_PENALTY=6.016304
PRIMER_LEFT_23_PENALTY=0.227274
PRIMER_RIGHT_23_PENALTY=5.789030
PRIMER_LEFT_23_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_23_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_23=898,20
PRIMER_RIGHT_23=1870,24
PRIMER_LEFT_23_TM=59.773
PRIMER_RIGHT_23_TM=58.211
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=41.667
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=973
PRIMER_PAIR_24_PENALTY=6.043136
PRIMER_LEFT_24_PENALTY=1.730588
PRIMER_RIGHT_24_PENALTY=4.312548
PRIMER_LEFT_24_SEQUENCE=CTCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_24_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_24=889,21
PRIMER_RIGHT_24=1861,24
PRIMER_LEFT_24_TM=59.269
PRIMER_RIGHT_24_TM=60.313
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=45.833
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=973
PRIMER_PAIR_25_PENALTY=6.061196
PRIMER_LEFT_25_PENALTY=0.745029
PRIMER_RIGHT_25_PENALTY=5.316167
PRIMER_LEFT_25_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_25_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAGA
PRIMER_LEFT_25=897,20
PRIMER_RIGHT_25=1868,25
PRIMER_LEFT_25_TM=59.255
PRIMER_RIGHT_25_TM=60.316
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=44.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=972
PRIMER_PAIR_26_PENALTY=6.111421
PRIMER_LEFT_26_PENALTY=1.798872
PRIMER_RIGHT_26_PENALTY=4.312548
PRIMER_LEFT_26_SEQUENCE=TCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_26_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_26=890,20
PRIMER_RIGHT_26=1861,24
PRIMER_LEFT_26_TM=58.201
PRIMER_RIGHT_26_TM=60.313
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.833
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=972
PRIMER_PAIR_27_PENALTY=6.128637
PRIMER_LEFT_27_PENALTY=0.745029
PRIMER_RIGHT_27_PENALTY=5.383607
PRIMER_LEFT_27_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_27_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_27=897,20
PRIMER_RIGHT_27=1869,24
PRIMER_LEFT_27_TM=59.255
PRIMER_RIGHT_27_TM=58.616
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=41.667
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=973
PRIMER_PAIR_28_PENALTY=6.134386
PRIMER_LEFT_28_PENALTY=1.730588
PRIMER_RIGHT_28_PENALTY=4.403798
PRIMER_LEFT_28_SEQUENCE=CTCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_28_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_28=889,21
PRIMER_RIGHT_28=1876,22
PRIMER_LEFT_28_TM=59.269
PRIMER_RIGHT_28_TM=57.596
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=8.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=988
PRIMER_PAIR_29_PENALTY=6.139153
PRIMER_LEFT_29_PENALTY=1.826605
PRIMER_RIGHT_29_PENALTY=4.312548
PRIMER_LEFT_29_SEQUENCE=CCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_29_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_29=900,20
PRIMER_RIGHT_29=1861,24
PRIMER_LEFT_29_TM=58.173
PRIMER_RIGHT_29_TM=60.313
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.833
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=962
PRIMER_PAIR_30_PENALTY=6.158546
PRIMER_LEFT_30_PENALTY=0.227274
PRIMER_RIGHT_30_PENALTY=5.931273
PRIMER_LEFT_30_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_30_SEQUENCE=CGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_30=898,20
PRIMER_RIGHT_30=1868,22
PRIMER_LEFT_30_TM=59.773
PRIMER_RIGHT_30_TM=56.069
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=971
PRIMER_PAIR_31_PENALTY=6.202670
PRIMER_LEFT_31_PENALTY=1.798872
PRIMER_RIGHT_31_PENALTY=4.403798
PRIMER_LEFT_31_SEQUENCE=TCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_31_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_31=890,20
PRIMER_RIGHT_31=1876,22
PRIMER_LEFT_31_TM=58.201
PRIMER_RIGHT_31_TM=57.596
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=987
PRIMER_PAIR_32_PENALTY=6.230403
PRIMER_LEFT_32_PENALTY=1.826605
PRIMER_RIGHT_32_PENALTY=4.403798
PRIMER_LEFT_32_SEQUENCE=CCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_32_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_32=900,20
PRIMER_RIGHT_32=1876,22
PRIMER_LEFT_32_TM=58.173
PRIMER_RIGHT_32_TM=57.596
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=977
PRIMER_PAIR_33_PENALTY=6.230794
PRIMER_LEFT_33_PENALTY=1.059316
PRIMER_RIGHT_33_PENALTY=5.171477
PRIMER_LEFT_33_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_33_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_33=899,21
PRIMER_RIGHT_33=1868,23
PRIMER_LEFT_33_TM=60.059
PRIMER_RIGHT_33_TM=57.829
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=43.478
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=970
PRIMER_PAIR_34_PENALTY=6.272577
PRIMER_LEFT_34_PENALTY=1.960028
PRIMER_RIGHT_34_PENALTY=4.312548
PRIMER_LEFT_34_SEQUENCE=AAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_34_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_34=884,20
PRIMER_RIGHT_34=1861,24
PRIMER_LEFT_34_TM=58.040
PRIMER_RIGHT_34_TM=60.313
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.833
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=978
PRIMER_PAIR_35_PENALTY=6.277995
PRIMER_LEFT_35_PENALTY=0.745029
PRIMER_RIGHT_35_PENALTY=5.532965
PRIMER_LEFT_35_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_35_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_35=897,20
PRIMER_RIGHT_35=1869,25
PRIMER_LEFT_35_TM=59.255
PRIMER_RIGHT_35_TM=59.467
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=44.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=973
PRIMER_PAIR_36_PENALTY=6.279436
PRIMER_LEFT_36_PENALTY=1.059316
PRIMER_RIGHT_36_PENALTY=5.220120
PRIMER_LEFT_36_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_36_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_36=899,21
PRIMER_RIGHT_36=1870,25
PRIMER_LEFT_36_TM=60.059
PRIMER_RIGHT_36_TM=59.780
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=972
PRIMER_PAIR_37_PENALTY=6.287644
PRIMER_LEFT_37_PENALTY=0.227274
PRIMER_RIGHT_37_PENALTY=6.060370
PRIMER_LEFT_37_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_37_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_37=898,20
PRIMER_RIGHT_37=1869,23
PRIMER_LEFT_37_TM=59.773
PRIMER_RIGHT_37_TM=56.940
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=43.478
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=972
PRIMER_PAIR_38_PENALTY=6.319374
PRIMER_LEFT_38_PENALTY=1.059316
PRIMER_RIGHT_38_PENALTY=5.260057
PRIMER_LEFT_38_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_38_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_38=899,21
PRIMER_RIGHT_38=1868,24
PRIMER_LEFT_38_TM=60.059
PRIMER_RIGHT_38_TM=58.740
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=45.833
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=970
PRIMER_PAIR_39_PENALTY=6.351709
PRIMER_LEFT_39_PENALTY=0.227274
PRIMER_RIGHT_39_PENALTY=6.124435
PRIMER_LEFT_39_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_39_SEQUENCE=AGCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_39=898,20
PRIMER_RIGHT_39=1862,25
PRIMER_LEFT_39_TM=59.773
PRIMER_RIGHT_39_TM=61.124
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=44.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=965
PRIMER_PAIR_40_PENALTY=6.363826
PRIMER_LEFT_40_PENALTY=1.960028
PRIMER_RIGHT_40_PENALTY=4.403798
PRIMER_LEFT_40_SEQUENCE=AAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_40_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_40=884,20
PRIMER_RIGHT_40=1876,22
PRIMER_LEFT_40_TM=58.040
PRIMER_RIGHT_40_TM=57.596
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=993
PRIMER_PAIR_41_PENALTY=6.375483
PRIMER_LEFT_41_PENALTY=1.059316
PRIMER_RIGHT_41_PENALTY=5.316167
PRIMER_LEFT_41_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_41_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAGA
PRIMER_LEFT_41=899,21
PRIMER_RIGHT_41=1868,25
PRIMER_LEFT_41_TM=60.059
PRIMER_RIGHT_41_TM=60.316
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=44.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=970
PRIMER_PAIR_42_PENALTY=6.410738
PRIMER_LEFT_42_PENALTY=0.227274
PRIMER_RIGHT_42_PENALTY=6.183465
PRIMER_LEFT_42_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_42_SEQUENCE=CTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_42=898,20
PRIMER_RIGHT_42=1860,23
PRIMER_LEFT_42_TM=59.773
PRIMER_RIGHT_42_TM=56.817
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=963
PRIMER_PAIR_43_PENALTY=6.420066
PRIMER_LEFT_43_PENALTY=2.107518
PRIMER_RIGHT_43_PENALTY=4.312548
PRIMER_LEFT_43_SEQUENCE=TTTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_43_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_43=897,21
PRIMER_RIGHT_43=1861,24
PRIMER_LEFT_43_TM=61.108
PRIMER_RIGHT_43_TM=60.313
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.833
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=965
PRIMER_PAIR_44_PENALTY=6.442924
PRIMER_LEFT_44_PENALTY=1.059316
PRIMER_RIGHT_44_PENALTY=5.383607
PRIMER_LEFT_44_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_44_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_44=899,21
PRIMER_RIGHT_44=1869,24
PRIMER_LEFT_44_TM=60.059
PRIMER_RIGHT_44_TM=58.616
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=971
PRIMER_PAIR_45_PENALTY=6.511316
PRIMER_LEFT_45_PENALTY=2.107518
PRIMER_RIGHT_45_PENALTY=4.403798
PRIMER_LEFT_45_SEQUENCE=TTTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_45_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_45=897,21
PRIMER_RIGHT_45=1876,22
PRIMER_LEFT_45_TM=61.108
PRIMER_RIGHT_45_TM=57.596
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=980
PRIMER_PAIR_46_PENALTY=6.512234
PRIMER_LEFT_46_PENALTY=0.227274
PRIMER_RIGHT_46_PENALTY=6.284961
PRIMER_LEFT_46_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_46_SEQUENCE=TCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_46=898,20
PRIMER_RIGHT_46=1859,22
PRIMER_LEFT_46_TM=59.773
PRIMER_RIGHT_46_TM=55.715
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=962
PRIMER_PAIR_47_PENALTY=6.515650
PRIMER_LEFT_47_PENALTY=1.344173
PRIMER_RIGHT_47_PENALTY=5.171477
PRIMER_LEFT_47_SEQUENCE=TCCCAGATTTCCAGAAGACAA
PRIMER_RIGHT_47_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_47=890,21
PRIMER_RIGHT_47=1868,23
PRIMER_LEFT_47_TM=59.656
PRIMER_RIGHT_47_TM=57.829
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=979
PRIMER_PAIR_48_PENALTY=6.534060
PRIMER_LEFT_48_PENALTY=0.745029
PRIMER_RIGHT_48_PENALTY=5.789030
PRIMER_LEFT_48_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_48_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_48=897,20
PRIMER_RIGHT_48=1870,24
PRIMER_LEFT_48_TM=59.255
PRIMER_RIGHT_48_TM=58.211
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=41.667
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=974
PRIMER_PAIR_49_PENALTY=6.536871
PRIMER_LEFT_49_PENALTY=2.224322
PRIMER_RIGHT_49_PENALTY=4.312548
PRIMER_LEFT_49_SEQUENCE=CAGATTTCCAGAAGACAAGGG
PRIMER_RIGHT_49_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_49=893,21
PRIMER_RIGHT_49=1861,24
PRIMER_LEFT_49_TM=58.776
PRIMER_RIGHT_49_TM=60.313
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=45.833
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=969
=
SEQUENCE_ID=MAGIC.338_1_16526784_16527452_edi-0_can-0_13120_2522223_TRUE
SEQUENCE_TARGET=950,768
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4729906_4730148_ct-1_oy-0_8549_37657_TRUE
SEQUENCE_TARGET=950,342
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCNAAGNNTTTATAAGTAAATCNAAATAAACACCTAAACANGTGCTANATCACANNNNNNNNNNNNNNNNNNNNNNNNNNNATCNTNGNGAAACGGCCGTACNTACCCCTCAAATCCATTGGNTCCTTNTNCNACTTGGTNANCTATCAAANNATAATNTTACTCAATTANGGACTAATCGGCGGTTACTAACANTAACTANNGTNGGAAAACTTANNGACTTCCGAATNNGAACTTTTNGCNGGNTNNNTNANCNATGCTAACTTNGAGCGANTTTTGGGTGGAGNTTTCCTAATGANNNNNNNNNNNNNNNTAGGGTTTTAGGTNGGTTTNTCAAGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGANACATGGCNTCGAGTCACNGCTNGAGACACCCANNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGATGCNGAGGTGGCGACAGCCGGNCNNGAGTCACNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNGGNCGATCGGCANCCNCANGATTATGGNNGCCCGANATNNNCGATCCTNAGCCANANAACACTGAANNAGCTTACCCACCTNCTTNTNNCCTNAAGCTGGTCGNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_15788829_15789383_sf-2_bur-0_30397_58848_TRUE
SEQUENCE_TARGET=950,654
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_6737814_6738111_wil-2_po-0_13465_18508_TRUE
SEQUENCE_TARGET=950,397
SEQUENCE_TEMPLATE=TTCTGTCTGATTANTTGGGTNTTTAAACCAAACCAAAACCGTGTTAAAAATCCTGGCACATTTCATTTATAATGATNCAACCACCAACCTANNNNNNNNTTAATTTNTTNTTATTTATTTTTAACAATCATCATTAGATACTATAATAAACTAATTTATCAATTTGAANGAACCCAATAATTGTCTNTCTTTNTTTGTATTAATTCTAATCATTNTTGTTAGTTATATGANAAATNGAAGTAAANTATTTCAAACCTCAAAATGATTTGNCNTAATTCAAAATAAAGTATTATGCCAANCATTTGNNNNNNTGAAGTCTTCAACGAAAATAAAACTGCAANCTTATTGCAATGTGGTACCCCAATCATNAAAAAAANTCANAATCCAAAACTTAATATTTATATTANTTNTTAGAATTGGATTTAAAAATTATCAGATAATGATATAGTTATAATAAATCGGCTTCTAAATACTATAATAAACCTTTCTCATCTCGAAAAGACGTTTCTATAAACTCTTACGCGTTGTATAATTGATCTGATTCACCAATTCTTNTNATGGATGTGATTAACCTGATCCAAAAGAACTTTATAGAAAATTNNTTAAGGACAAGNTTAATCACATTTGTAATTTANTTATTTTGTTCTCTNGTATATGCTTATTTTGTTTAACATTATTANCTGCATTTNAATAGCTATTTAGATTATATTTTANTGGCTATTCATGCACTTAAANACAAACCGTGATGAGCCTGATGGCATAGNCCGATCCTTANTGACTTTTGCATTCCTACCATGCCAANTTCCAATCATTGGAGTGCATGTTTTCATTATTTCNTATCTAGCATTTAGGAGAATTNTCACTGATCTAAACACAACCTCAGNAAATTTTAACAACTTATAGACATCTTCAACTTTTCATGAGCATAGAATCTTTATAATATTATATGNAATATGTGCACTATATTAGATAACAATNATCACACAATATAAANTCNATNNNTTGATATAANTTTGTGCAAGTATAAAANTAAAACATATAATGCTGAGATTTTACANATGAAATGAAAACATATCAAATTTGAAGAATCACTTTGTAAATNAAAAAGAAGTTTCACACTTAAAATAGANTAATGAATTGTNTTATTCCAAATTTAGAATANNACACTACTTTTNACNCTATTTTNGGAATGCGANTTGGAGATNATGCTCTTAAACTACCGGGTTTCAAGCCGCGAACTCACTCAATCACTTAGCTTTTAATTCAAANATAGCAATCTGTCCACGAGAGAGCGAGANAGAACAAAATGACGTCGCAGAAAANATTCATCTTTGAGGTGGAAGCCGCTAAGGAAGCCACAGATGGAAATCCCTCNGTTGGTCCTGTCTATCGTAGTACTTTTGCTCAGAACGGATTCCCNAACCCGATCGATGGTATCCAAAGCTGCTGGGATATTTTCCGGTCNGAATCTCTTTTCTTTTAATGTGTTCTATTGTTTAANTCTATTNNTTTTTTTTNTCTCCATGCATTGCTATTTGNACGACATTTTGTGTGCTCATATACGTTTTGATTTAGAGTGTAGCATGCATTATCATTTTGAAACATGTTAATNTGATAAACGTATATCGATCAGGGATCTTGGGAATTTTATTTCATGTTTTGTACGTAAGAATGATTACTAAGTTATATCTTTGGCTCAAAATGAATGAATACTGAATTGTTTGGTGTNAATTCTTGTAAGATCCATTTTTAGCTTCTTTTCTTGCTCTTACGTGATGGTTTTGTCTGACATGGTACATGGCANTATTTAAATAATTTTGGGGTGGCCTTCTATATTAATTGCAATCGGTCGNTTAAAGATTTTGTTTAACGATATATAGTTCTTTCTCTAACAANNTTTTAACTTTTTATTATTNTCCAGTAGCTTCTGAATTTTAANAATAAATGTNNTTNTTTTTTNTTNTATTGCGNNNNNNNNNNTGCAGCACGGCTGTTNAGAANTATCCAAACAATCGAATGCTTGGTCGCCGTGAGATTTCGAACGGGAAGGTGANTTGTTNTCTTTTCATTTNTTTTGTANNNNNNNNNNNNATTTAGTGTTTTCGAGAGGATAGNTAATATCTTTCTGCCCTTTTTCTATAATTCTTAGGCAGGAAAGTACGTGTGGAAAACATACAAAGAAGTATACGACATTGTCATAAAACTTGGAAATTCTCTACGTAGTTGCGGGATTAAGGAGGTTCAAATTCATATTTACTATATATTTTTCATATGTCCTCAAATAATGGTTTTAGTGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.166227
PRIMER_LEFT_0_PENALTY=0.836677
PRIMER_RIGHT_0_PENALTY=0.329550
PRIMER_LEFT_0_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_0_SEQUENCE=TTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_0=779,20
PRIMER_RIGHT_0=1367,20
PRIMER_LEFT_0_TM=60.837
PRIMER_RIGHT_0_TM=59.670
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=589
PRIMER_PAIR_1_PENALTY=1.348194
PRIMER_LEFT_1_PENALTY=0.836677
PRIMER_RIGHT_1_PENALTY=0.511517
PRIMER_LEFT_1_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_1_SEQUENCE=GATTTCCATCTGTGGCTTCC
PRIMER_LEFT_1=779,20
PRIMER_RIGHT_1=1369,20
PRIMER_LEFT_1_TM=60.837
PRIMER_RIGHT_1_TM=59.488
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=591
PRIMER_PAIR_2_PENALTY=1.348194
PRIMER_LEFT_2_PENALTY=0.836677
PRIMER_RIGHT_2_PENALTY=0.511517
PRIMER_LEFT_2_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_2_SEQUENCE=GGATTTCCATCTGTGGCTTC
PRIMER_LEFT_2=779,20
PRIMER_RIGHT_2=1370,20
PRIMER_LEFT_2_TM=60.837
PRIMER_RIGHT_2_TM=59.488
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=592
PRIMER_PAIR_3_PENALTY=1.689292
PRIMER_LEFT_3_PENALTY=0.836677
PRIMER_RIGHT_3_PENALTY=0.852615
PRIMER_LEFT_3_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_3_SEQUENCE=AGGGATTTCCATCTGTGGCT
PRIMER_LEFT_3=779,20
PRIMER_RIGHT_3=1372,20
PRIMER_LEFT_3_TM=60.837
PRIMER_RIGHT_3_TM=60.853
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=594
PRIMER_PAIR_4_PENALTY=1.882508
PRIMER_LEFT_4_PENALTY=0.836677
PRIMER_RIGHT_4_PENALTY=1.045831
PRIMER_LEFT_4_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_4_SEQUENCE=ATTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_4=779,20
PRIMER_RIGHT_4=1368,21
PRIMER_LEFT_4_TM=60.837
PRIMER_RIGHT_4_TM=59.954
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=590
PRIMER_PAIR_5_PENALTY=2.059375
PRIMER_LEFT_5_PENALTY=0.836677
PRIMER_RIGHT_5_PENALTY=1.222698
PRIMER_LEFT_5_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_5_SEQUENCE=GGGATTTCCATCTGTGGCTT
PRIMER_LEFT_5=779,20
PRIMER_RIGHT_5=1371,20
PRIMER_LEFT_5_TM=60.837
PRIMER_RIGHT_5_TM=61.223
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=593
PRIMER_PAIR_6_PENALTY=2.230297
PRIMER_LEFT_6_PENALTY=1.900748
PRIMER_RIGHT_6_PENALTY=0.329550
PRIMER_LEFT_6_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_6_SEQUENCE=TTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_6=809,20
PRIMER_RIGHT_6=1367,20
PRIMER_LEFT_6_TM=58.099
PRIMER_RIGHT_6_TM=59.670
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=559
PRIMER_PAIR_7_PENALTY=2.241199
PRIMER_LEFT_7_PENALTY=0.836677
PRIMER_RIGHT_7_PENALTY=1.404522
PRIMER_LEFT_7_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_7_SEQUENCE=ATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_7=779,20
PRIMER_RIGHT_7=1368,20
PRIMER_LEFT_7_TM=60.837
PRIMER_RIGHT_7_TM=58.595
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=590
PRIMER_PAIR_8_PENALTY=2.294288
PRIMER_LEFT_8_PENALTY=0.836677
PRIMER_RIGHT_8_PENALTY=1.457611
PRIMER_LEFT_8_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_8_SEQUENCE=GATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_8=779,20
PRIMER_RIGHT_8=1369,21
PRIMER_LEFT_8_TM=60.837
PRIMER_RIGHT_8_TM=60.458
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=591
PRIMER_PAIR_9_PENALTY=2.410132
PRIMER_LEFT_9_PENALTY=0.836677
PRIMER_RIGHT_9_PENALTY=1.573455
PRIMER_LEFT_9_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_9_SEQUENCE=AAAATTCCCAAGATCCCTGA
PRIMER_LEFT_9=779,20
PRIMER_RIGHT_9=1648,20
PRIMER_LEFT_9_TM=60.837
PRIMER_RIGHT_9_TM=58.427
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=870
PRIMER_PAIR_10_PENALTY=2.412264
PRIMER_LEFT_10_PENALTY=1.900748
PRIMER_RIGHT_10_PENALTY=0.511517
PRIMER_LEFT_10_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_10_SEQUENCE=GATTTCCATCTGTGGCTTCC
PRIMER_LEFT_10=809,20
PRIMER_RIGHT_10=1369,20
PRIMER_LEFT_10_TM=58.099
PRIMER_RIGHT_10_TM=59.488
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=561
PRIMER_PAIR_11_PENALTY=2.412264
PRIMER_LEFT_11_PENALTY=1.900748
PRIMER_RIGHT_11_PENALTY=0.511517
PRIMER_LEFT_11_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_11_SEQUENCE=GGATTTCCATCTGTGGCTTC
PRIMER_LEFT_11=809,20
PRIMER_RIGHT_11=1370,20
PRIMER_LEFT_11_TM=58.099
PRIMER_RIGHT_11_TM=59.488
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=562
PRIMER_PAIR_12_PENALTY=2.753363
PRIMER_LEFT_12_PENALTY=1.900748
PRIMER_RIGHT_12_PENALTY=0.852615
PRIMER_LEFT_12_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_12_SEQUENCE=AGGGATTTCCATCTGTGGCT
PRIMER_LEFT_12=809,20
PRIMER_RIGHT_12=1372,20
PRIMER_LEFT_12_TM=58.099
PRIMER_RIGHT_12_TM=60.853
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=564
PRIMER_PAIR_13_PENALTY=2.915428
PRIMER_LEFT_13_PENALTY=0.836677
PRIMER_RIGHT_13_PENALTY=2.078751
PRIMER_LEFT_13_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_13_SEQUENCE=GACCGGAAAATATCCCAGCA
PRIMER_LEFT_13=779,20
PRIMER_RIGHT_13=1463,20
PRIMER_LEFT_13_TM=60.837
PRIMER_RIGHT_13_TM=62.079
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=685
PRIMER_PAIR_14_PENALTY=2.946579
PRIMER_LEFT_14_PENALTY=1.900748
PRIMER_RIGHT_14_PENALTY=1.045831
PRIMER_LEFT_14_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_14_SEQUENCE=ATTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_14=809,20
PRIMER_RIGHT_14=1368,21
PRIMER_LEFT_14_TM=58.099
PRIMER_RIGHT_14_TM=59.954
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=560
PRIMER_PAIR_15_PENALTY=3.035002
PRIMER_LEFT_15_PENALTY=2.705452
PRIMER_RIGHT_15_PENALTY=0.329550
PRIMER_LEFT_15_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_15_SEQUENCE=TTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_15=778,21
PRIMER_RIGHT_15=1367,20
PRIMER_LEFT_15_TM=61.705
PRIMER_RIGHT_15_TM=59.670
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=590
PRIMER_PAIR_16_PENALTY=3.123445
PRIMER_LEFT_16_PENALTY=1.900748
PRIMER_RIGHT_16_PENALTY=1.222698
PRIMER_LEFT_16_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_16_SEQUENCE=GGGATTTCCATCTGTGGCTT
PRIMER_LEFT_16=809,20
PRIMER_RIGHT_16=1371,20
PRIMER_LEFT_16_TM=58.099
PRIMER_RIGHT_16_TM=61.223
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=563
PRIMER_PAIR_17_PENALTY=3.216969
PRIMER_LEFT_17_PENALTY=2.705452
PRIMER_RIGHT_17_PENALTY=0.511517
PRIMER_LEFT_17_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_17_SEQUENCE=GATTTCCATCTGTGGCTTCC
PRIMER_LEFT_17=778,21
PRIMER_RIGHT_17=1369,20
PRIMER_LEFT_17_TM=61.705
PRIMER_RIGHT_17_TM=59.488
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=592
PRIMER_PAIR_18_PENALTY=3.216969
PRIMER_LEFT_18_PENALTY=2.705452
PRIMER_RIGHT_18_PENALTY=0.511517
PRIMER_LEFT_18_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_18_SEQUENCE=GGATTTCCATCTGTGGCTTC
PRIMER_LEFT_18=778,21
PRIMER_RIGHT_18=1370,20
PRIMER_LEFT_18_TM=61.705
PRIMER_RIGHT_18_TM=59.488
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.6000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=593
PRIMER_PAIR_19_PENALTY=3.305269
PRIMER_LEFT_19_PENALTY=1.900748
PRIMER_RIGHT_19_PENALTY=1.404522
PRIMER_LEFT_19_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_19_SEQUENCE=ATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_19=809,20
PRIMER_RIGHT_19=1368,20
PRIMER_LEFT_19_TM=58.099
PRIMER_RIGHT_19_TM=58.595
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.3000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=560
PRIMER_PAIR_20_PENALTY=3.358359
PRIMER_LEFT_20_PENALTY=1.900748
PRIMER_RIGHT_20_PENALTY=1.457611
PRIMER_LEFT_20_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_20_SEQUENCE=GATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_20=809,20
PRIMER_RIGHT_20=1369,21
PRIMER_LEFT_20_TM=58.099
PRIMER_RIGHT_20_TM=60.458
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.3000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=561
PRIMER_PAIR_21_PENALTY=3.387127
PRIMER_LEFT_21_PENALTY=0.836677
PRIMER_RIGHT_21_PENALTY=2.550450
PRIMER_LEFT_21_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_21_SEQUENCE=GAATCCGTTCTGAGCAAAAGT
PRIMER_LEFT_21=779,20
PRIMER_RIGHT_21=1416,21
PRIMER_LEFT_21_TM=60.837
PRIMER_RIGHT_21_TM=58.450
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=638
PRIMER_PAIR_22_PENALTY=3.434786
PRIMER_LEFT_22_PENALTY=0.836677
PRIMER_RIGHT_22_PENALTY=2.598109
PRIMER_LEFT_22_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_22_SEQUENCE=CCATCACGTAAGAGCAAGAAAA
PRIMER_LEFT_22=779,20
PRIMER_RIGHT_22=1778,22
PRIMER_LEFT_22_TM=60.837
PRIMER_RIGHT_22_TM=59.402
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=1000
PRIMER_PAIR_23_PENALTY=3.474202
PRIMER_LEFT_23_PENALTY=1.900748
PRIMER_RIGHT_23_PENALTY=1.573455
PRIMER_LEFT_23_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_23_SEQUENCE=AAAATTCCCAAGATCCCTGA
PRIMER_LEFT_23=809,20
PRIMER_RIGHT_23=1648,20
PRIMER_LEFT_23_TM=58.099
PRIMER_RIGHT_23_TM=58.427
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=840
PRIMER_PAIR_24_PENALTY=3.548365
PRIMER_LEFT_24_PENALTY=3.218816
PRIMER_RIGHT_24_PENALTY=0.329550
PRIMER_LEFT_24_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_24_SEQUENCE=TTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_24=775,20
PRIMER_RIGHT_24=1367,20
PRIMER_LEFT_24_TM=56.781
PRIMER_RIGHT_24_TM=59.670
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.2000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=593
PRIMER_PAIR_25_PENALTY=3.558067
PRIMER_LEFT_25_PENALTY=2.705452
PRIMER_RIGHT_25_PENALTY=0.852615
PRIMER_LEFT_25_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_25_SEQUENCE=AGGGATTTCCATCTGTGGCT
PRIMER_LEFT_25=778,21
PRIMER_RIGHT_25=1372,20
PRIMER_LEFT_25_TM=61.705
PRIMER_RIGHT_25_TM=60.853
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=595
PRIMER_PAIR_26_PENALTY=3.730332
PRIMER_LEFT_26_PENALTY=3.218816
PRIMER_RIGHT_26_PENALTY=0.511517
PRIMER_LEFT_26_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_26_SEQUENCE=GATTTCCATCTGTGGCTTCC
PRIMER_LEFT_26=775,20
PRIMER_RIGHT_26=1369,20
PRIMER_LEFT_26_TM=56.781
PRIMER_RIGHT_26_TM=59.488
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.2000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=595
PRIMER_PAIR_27_PENALTY=3.730332
PRIMER_LEFT_27_PENALTY=3.218816
PRIMER_RIGHT_27_PENALTY=0.511517
PRIMER_LEFT_27_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_27_SEQUENCE=GGATTTCCATCTGTGGCTTC
PRIMER_LEFT_27=775,20
PRIMER_RIGHT_27=1370,20
PRIMER_LEFT_27_TM=56.781
PRIMER_RIGHT_27_TM=59.488
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.2000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=596
PRIMER_PAIR_28_PENALTY=3.751283
PRIMER_LEFT_28_PENALTY=2.705452
PRIMER_RIGHT_28_PENALTY=1.045831
PRIMER_LEFT_28_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_28_SEQUENCE=ATTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_28=778,21
PRIMER_RIGHT_28=1368,21
PRIMER_LEFT_28_TM=61.705
PRIMER_RIGHT_28_TM=59.954
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.6000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=591
PRIMER_PAIR_29_PENALTY=3.796111
PRIMER_LEFT_29_PENALTY=0.836677
PRIMER_RIGHT_29_PENALTY=2.959434
PRIMER_LEFT_29_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_29_SEQUENCE=CCATCACGTAAGAGCAAGAAA
PRIMER_LEFT_29=779,20
PRIMER_RIGHT_29=1778,21
PRIMER_LEFT_29_TM=60.837
PRIMER_RIGHT_29_TM=58.041
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.6000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=1000
PRIMER_PAIR_30_PENALTY=3.922200
PRIMER_LEFT_30_PENALTY=0.836677
PRIMER_RIGHT_30_PENALTY=3.085523
PRIMER_LEFT_30_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_30_SEQUENCE=AGGGATTTCCATCTGTGGCTT
PRIMER_LEFT_30=779,20
PRIMER_RIGHT_30=1372,21
PRIMER_LEFT_30_TM=60.837
PRIMER_RIGHT_30_TM=62.086
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=594
PRIMER_PAIR_31_PENALTY=3.928150
PRIMER_LEFT_31_PENALTY=2.705452
PRIMER_RIGHT_31_PENALTY=1.222698
PRIMER_LEFT_31_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_31_SEQUENCE=GGGATTTCCATCTGTGGCTT
PRIMER_LEFT_31=778,21
PRIMER_RIGHT_31=1371,20
PRIMER_LEFT_31_TM=61.705
PRIMER_RIGHT_31_TM=61.223
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=594
PRIMER_PAIR_32_PENALTY=4.066289
PRIMER_LEFT_32_PENALTY=1.900748
PRIMER_RIGHT_32_PENALTY=2.165541
PRIMER_LEFT_32_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_32_SEQUENCE=CAAAACCATCACGTAAGAGCAA
PRIMER_LEFT_32=809,20
PRIMER_RIGHT_32=1783,22
PRIMER_LEFT_32_TM=58.099
PRIMER_RIGHT_32_TM=60.166
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=975
PRIMER_PAIR_33_PENALTY=4.067362
PRIMER_LEFT_33_PENALTY=1.900748
PRIMER_RIGHT_33_PENALTY=2.166614
PRIMER_LEFT_33_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_33_SEQUENCE=CAAAACCATCACGTAAGAGCA
PRIMER_LEFT_33=809,20
PRIMER_RIGHT_33=1783,21
PRIMER_LEFT_33_TM=58.099
PRIMER_RIGHT_33_TM=58.833
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=975
PRIMER_PAIR_34_PENALTY=4.071430
PRIMER_LEFT_34_PENALTY=3.218816
PRIMER_RIGHT_34_PENALTY=0.852615
PRIMER_LEFT_34_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_34_SEQUENCE=AGGGATTTCCATCTGTGGCT
PRIMER_LEFT_34=775,20
PRIMER_RIGHT_34=1372,20
PRIMER_LEFT_34_TM=56.781
PRIMER_RIGHT_34_TM=60.853
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.2000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=598
PRIMER_PAIR_35_PENALTY=4.109974
PRIMER_LEFT_35_PENALTY=2.705452
PRIMER_RIGHT_35_PENALTY=1.404522
PRIMER_LEFT_35_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_35_SEQUENCE=ATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_35=778,21
PRIMER_RIGHT_35=1368,20
PRIMER_LEFT_35_TM=61.705
PRIMER_RIGHT_35_TM=58.595
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.6000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=591
PRIMER_PAIR_36_PENALTY=4.131217
PRIMER_LEFT_36_PENALTY=0.836677
PRIMER_RIGHT_36_PENALTY=3.294540
PRIMER_LEFT_36_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_36_SEQUENCE=CCATCACGTAAGAGCAAGAAAAG
PRIMER_LEFT_36=779,20
PRIMER_RIGHT_36=1778,23
PRIMER_LEFT_36_TM=60.837
PRIMER_RIGHT_36_TM=60.295
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.6000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=1000
PRIMER_PAIR_37_PENALTY=4.163063
PRIMER_LEFT_37_PENALTY=2.705452
PRIMER_RIGHT_37_PENALTY=1.457611
PRIMER_LEFT_37_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_37_SEQUENCE=GATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_37=778,21
PRIMER_RIGHT_37=1369,21
PRIMER_LEFT_37_TM=61.705
PRIMER_RIGHT_37_TM=60.458
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=592
PRIMER_PAIR_38_PENALTY=4.224103
PRIMER_LEFT_38_PENALTY=1.900748
PRIMER_RIGHT_38_PENALTY=2.323355
PRIMER_LEFT_38_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_38_SEQUENCE=ACAAAACCATCACGTAAGAGCA
PRIMER_LEFT_38=809,20
PRIMER_RIGHT_38=1784,22
PRIMER_LEFT_38_TM=58.099
PRIMER_RIGHT_38_TM=59.677
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.3000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=976
PRIMER_PAIR_39_PENALTY=4.264647
PRIMER_LEFT_39_PENALTY=3.218816
PRIMER_RIGHT_39_PENALTY=1.045831
PRIMER_LEFT_39_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_39_SEQUENCE=ATTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_39=775,20
PRIMER_RIGHT_39=1368,21
PRIMER_LEFT_39_TM=56.781
PRIMER_RIGHT_39_TM=59.954
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=594
PRIMER_PAIR_40_PENALTY=4.278907
PRIMER_LEFT_40_PENALTY=2.705452
PRIMER_RIGHT_40_PENALTY=1.573455
PRIMER_LEFT_40_SEQUENCE=CTTTTGCATTCCTACCATGCC
PRIMER_RIGHT_40_SEQUENCE=AAAATTCCCAAGATCCCTGA
PRIMER_LEFT_40=778,21
PRIMER_RIGHT_40=1648,20
PRIMER_LEFT_40_TM=61.705
PRIMER_RIGHT_40_TM=58.427
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=871
PRIMER_PAIR_41_PENALTY=4.298280
PRIMER_LEFT_41_PENALTY=0.836677
PRIMER_RIGHT_41_PENALTY=3.461603
PRIMER_LEFT_41_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_41_SEQUENCE=AATCCGTTCTGAGCAAAAGT
PRIMER_LEFT_41=779,20
PRIMER_RIGHT_41=1415,20
PRIMER_LEFT_41_TM=60.837
PRIMER_RIGHT_41_TM=56.538
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.6000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=637
PRIMER_PAIR_42_PENALTY=4.314114
PRIMER_LEFT_42_PENALTY=0.836677
PRIMER_RIGHT_42_PENALTY=3.477437
PRIMER_LEFT_42_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_42_SEQUENCE=CCATCACGTAAGAGCAAGAA
PRIMER_LEFT_42=779,20
PRIMER_RIGHT_42=1778,20
PRIMER_LEFT_42_TM=60.837
PRIMER_RIGHT_42_TM=56.523
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.6000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=1000
PRIMER_PAIR_43_PENALTY=4.441513
PRIMER_LEFT_43_PENALTY=3.218816
PRIMER_RIGHT_43_PENALTY=1.222698
PRIMER_LEFT_43_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_43_SEQUENCE=GGGATTTCCATCTGTGGCTT
PRIMER_LEFT_43=775,20
PRIMER_RIGHT_43=1371,20
PRIMER_LEFT_43_TM=56.781
PRIMER_RIGHT_43_TM=61.223
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.2000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=597
PRIMER_PAIR_44_PENALTY=4.451198
PRIMER_LEFT_44_PENALTY=1.900748
PRIMER_RIGHT_44_PENALTY=2.550450
PRIMER_LEFT_44_SEQUENCE=CATTGGAGTGCATGTTTTCA
PRIMER_RIGHT_44_SEQUENCE=GAATCCGTTCTGAGCAAAAGT
PRIMER_LEFT_44=809,20
PRIMER_RIGHT_44=1416,21
PRIMER_LEFT_44_TM=58.099
PRIMER_RIGHT_44_TM=58.450
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=608
PRIMER_PAIR_45_PENALTY=4.472646
PRIMER_LEFT_45_PENALTY=0.836677
PRIMER_RIGHT_45_PENALTY=3.635969
PRIMER_LEFT_45_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_45_SEQUENCE=GAGGGATTTCCATCTGTGGCT
PRIMER_LEFT_45=779,20
PRIMER_RIGHT_45=1373,21
PRIMER_LEFT_45_TM=60.837
PRIMER_RIGHT_45_TM=62.636
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=595
PRIMER_PAIR_46_PENALTY=4.518767
PRIMER_LEFT_46_PENALTY=0.836677
PRIMER_RIGHT_46_PENALTY=3.682090
PRIMER_LEFT_46_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_46_SEQUENCE=GATTTCCATCTGTGGCTTCCTT
PRIMER_LEFT_46=779,20
PRIMER_RIGHT_46=1369,22
PRIMER_LEFT_46_TM=60.837
PRIMER_RIGHT_46_TM=61.682
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=591
PRIMER_PAIR_47_PENALTY=4.623337
PRIMER_LEFT_47_PENALTY=3.218816
PRIMER_RIGHT_47_PENALTY=1.404522
PRIMER_LEFT_47_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_47_SEQUENCE=ATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_47=775,20
PRIMER_RIGHT_47=1368,20
PRIMER_LEFT_47_TM=56.781
PRIMER_RIGHT_47_TM=58.595
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.2000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=594
PRIMER_PAIR_48_PENALTY=4.662561
PRIMER_LEFT_48_PENALTY=0.836677
PRIMER_RIGHT_48_PENALTY=3.825884
PRIMER_LEFT_48_SEQUENCE=TTTTGCATTCCTACCATGCC
PRIMER_RIGHT_48_SEQUENCE=GGAATCCGTTCTGAGCAAAAGT
PRIMER_LEFT_48=779,20
PRIMER_RIGHT_48=1417,22
PRIMER_LEFT_48_TM=60.837
PRIMER_RIGHT_48_TM=61.826
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=639
PRIMER_PAIR_49_PENALTY=4.676426
PRIMER_LEFT_49_PENALTY=3.218816
PRIMER_RIGHT_49_PENALTY=1.457611
PRIMER_LEFT_49_SEQUENCE=TGACTTTTGCATTCCTACCA
PRIMER_RIGHT_49_SEQUENCE=GATTTCCATCTGTGGCTTCCT
PRIMER_LEFT_49=775,20
PRIMER_RIGHT_49=1369,21
PRIMER_LEFT_49_TM=56.781
PRIMER_RIGHT_49_TM=60.458
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.2000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=595
=
SEQUENCE_ID=MAGIC.446_5_16053897_16054514_sf-2_hi-0_40035_46882_TRUE
SEQUENCE_TARGET=950,717
SEQUENCE_TEMPLATE=AAGNGGTTCAAGAATCTGTNACTAATTTTGGGTTGATGCAGCATGAACTTTATGGGTGTGATCAAAACATGTCTATGGGCAACATCATTAACAACAGCTTTCAACAGCGTCTCAAACACAGAACAAGAATCTGTGAATAACCNCTGGTTGAATCAGTTGATGNAGCATGAACTCTATGGCTATGATCAATGGTGAAGTAGCNNAACAGAGATAGTGAAGCTGTTTTCTTGAGTCTTGATTCACTTGTTAATTTTTATTTTTGAGTACGCTTGTTAATCTGTAGAGATTTCGTTCTGAGTTTTAAAGTTACAACTTGTATTTTCGGTTTAGTTATTACATATATATGATATAAAAACTCGAGCAATCTTTAACTAACATGTTAAATTGAGGNACATCGACGAAACTNANAACAACAAGCAATANAGTAGCAAGNAGCAGAAACAAGCAAAGCAATCAAACAATTGTGGTGATGAAACNTGTAGTAAAGNAAGAGAAGCTTGCCTATTAGTAACATCCAATTAGAATTGTATCACAANGTAGGGCAAGTAAANCATTTTTGACTAGTGTGTAACTTGTANNCTATCTCCATCACTAATGTTCACTCTCATCTCTGNAAATTATGATGGTGTAANAANANAACAATANTAAATTCTTCAANCAAAANATATCCTCGAATTNNAANTCTTCCAACGCAANTAAAATTTGANAACAAAAACTGACTGTTTTAGTCTGAAACACACATAANTAAANNNNNNNNNNNNNNNNNNNNNANNGGTGATTTACTCTNCATCTTCATGTCATCACCAGTCANCANNTGCTCCGTTCAATGCCTTAACTCTCTCGATCAACTTGTTCTTCCTCTCTNCGTACGTCAGTTTCTTCAAGTNGTANCTGTTTTGTTTNATANCACAATCANTCAAGAACCNAGTGAGACACACCAATCACAACAAGAAGATTAAAGNCAGTAATGATTATTTNTTTTACCTCTTGTGTTCCTTGGNAGCTGGTTTCTCGGTTTTCTTATGGTTGGGTTCTGCTCGGATAGCTGCGTGAACCTTCTTGTACATCTCCTCGATGCTCTCAGCTTCAACTCCTTTCTTAATGTAAGCACTGAAGAGAGTTTGTAACTTCTCTGGCTCATATTCCCCCAACAGCTTCATGTAGTTTGAGACGTGGCCACCGTAGATGTAGTTACTGTGGNTTTTATATGGTGCACCATTTTGCACTTGCAAGTGATCTTTGCTTCCGAGNTNNGGTTACTTTTAAGAGCANTTTTGTATCAACCCAACCTCTGATCCTTGTTATGGTGTACACAATATGATGATAAAGCTGCCGANTTTGGACAAGGCTTATTGTTNATGTNTTGCAATGAGAGAGGTAAGAAGTAGCTCTATGTATCATATGATTGTTGTTGTTAACCTATACTCAGCTAATAGANCAAAACTTTTCATTAATCATNCATAGATAACATAANTAAAAGCTAATTTTATATATTTCTGATTTCTCACTAGTCACTACGCATAATACATTCGTTGTTTTTGCTAAATATGAAATATCTATGAATTAAAGATTGAAAACAAAACGCGTTTACCAANTTACTATGCATTTTTAGATTAGNAAATATCTATGNATTAAAGNTTATATCGTAAAANATTTGACATTATTTTCACNTTTCGTTTTGGAGATAATGTTTTTNATATTGAGATNGACAAACATTTACAAAATATTTTTAGAATCTGGTTTCTAAGTGAGACTTTGCTTTTTGATTGTGNNNNAGACACGACTNNAAAAGTATTTATAGGCTTTAAGATTCAATGGAANTTCNACANAANAATAGTTGCATTGGCTTCGGCNCAAGATGAGATCATCAAANNNACATTCGTTAGTATAGTATACATACATGCAACAATCAACCATGGTCCATGTATGCATAGCGAAAATNTAACAAAGAAAAATGAGTAAGGTTGCANATAAAATAAANGTTTTTAAAGAAAATNGGTTATTCTTATTTNCTATAAATANAAANTTAGTGTATACGAAAATTTNAANCATGAATCAAACCAAATACGAAGGCAATTACTTCGAAATNNTATTTTGATANNNNNNNNNNNNNNNNTNAGTTTTGACGCATAATTGTTTCNAAGTTGAGAGTGAAGTGTGAACAGAATGCGTCTCTACTCTCTACTTCCTGATTTCTTTTCTGAAGAAANTTGGCACAACGAATATTAACTTTAGNAAAAAGGCAAAAAGAGTAAGCAAAAANCTTGAAATAAACAGCCTATAACCACTCTTCAAAGNTNNAAAGTCTAAAGNTTGTGTCTTNCTCATCATTGATTACACGTATACACATANATATTNATACNAAATACATACACTACCTATACATAGACCNATCTGTAGTCTTAGCAACTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=7.483751
PRIMER_LEFT_0_PENALTY=2.048588
PRIMER_RIGHT_0_PENALTY=5.435163
PRIMER_LEFT_0_SEQUENCE=TGAGACACACCAATCACAACAA
PRIMER_RIGHT_0_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_0=928,22
PRIMER_RIGHT_0=1760,25
PRIMER_LEFT_0_TM=60.049
PRIMER_RIGHT_0_TM=59.565
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=833
PRIMER_PAIR_1_PENALTY=7.587995
PRIMER_LEFT_1_PENALTY=2.152833
PRIMER_RIGHT_1_PENALTY=5.435163
PRIMER_LEFT_1_SEQUENCE=CCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_1_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_1=819,21
PRIMER_RIGHT_1=1760,25
PRIMER_LEFT_1_TM=58.847
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=942
PRIMER_PAIR_2_PENALTY=7.587995
PRIMER_LEFT_2_PENALTY=2.152833
PRIMER_RIGHT_2_PENALTY=5.435163
PRIMER_LEFT_2_SEQUENCE=TCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_2_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_2=818,21
PRIMER_RIGHT_2=1760,25
PRIMER_LEFT_2_TM=58.847
PRIMER_RIGHT_2_TM=59.565
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=943
PRIMER_PAIR_3_PENALTY=7.651750
PRIMER_LEFT_3_PENALTY=2.216588
PRIMER_RIGHT_3_PENALTY=5.435163
PRIMER_LEFT_3_SEQUENCE=CCGTTCAATGCCTTAACTCTCT
PRIMER_RIGHT_3_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_3=819,22
PRIMER_RIGHT_3=1760,25
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=59.565
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=942
PRIMER_PAIR_4_PENALTY=7.651750
PRIMER_LEFT_4_PENALTY=2.216588
PRIMER_RIGHT_4_PENALTY=5.435163
PRIMER_LEFT_4_SEQUENCE=CTCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_4_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_4=817,22
PRIMER_RIGHT_4=1760,25
PRIMER_LEFT_4_TM=59.783
PRIMER_RIGHT_4_TM=59.565
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=944
PRIMER_PAIR_5_PENALTY=7.658038
PRIMER_LEFT_5_PENALTY=2.048588
PRIMER_RIGHT_5_PENALTY=5.609449
PRIMER_LEFT_5_SEQUENCE=TGAGACACACCAATCACAACAA
PRIMER_RIGHT_5_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_5=928,22
PRIMER_RIGHT_5=1758,23
PRIMER_LEFT_5_TM=60.049
PRIMER_RIGHT_5_TM=57.391
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=831
PRIMER_PAIR_6_PENALTY=7.693574
PRIMER_LEFT_6_PENALTY=2.048588
PRIMER_RIGHT_6_PENALTY=5.644986
PRIMER_LEFT_6_SEQUENCE=TGAGACACACCAATCACAACAA
PRIMER_RIGHT_6_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_6=928,22
PRIMER_RIGHT_6=1759,24
PRIMER_LEFT_6_TM=60.049
PRIMER_RIGHT_6_TM=58.355
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=41.667
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=832
PRIMER_PAIR_7_PENALTY=7.711372
PRIMER_LEFT_7_PENALTY=2.048588
PRIMER_RIGHT_7_PENALTY=5.662784
PRIMER_LEFT_7_SEQUENCE=TGAGACACACCAATCACAACAA
PRIMER_RIGHT_7_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_7=928,22
PRIMER_RIGHT_7=1758,22
PRIMER_LEFT_7_TM=60.049
PRIMER_RIGHT_7_TM=56.337
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=831
PRIMER_PAIR_8_PENALTY=7.762282
PRIMER_LEFT_8_PENALTY=2.152833
PRIMER_RIGHT_8_PENALTY=5.609449
PRIMER_LEFT_8_SEQUENCE=CCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_8_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_8=819,21
PRIMER_RIGHT_8=1758,23
PRIMER_LEFT_8_TM=58.847
PRIMER_RIGHT_8_TM=57.391
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=940
PRIMER_PAIR_9_PENALTY=7.762282
PRIMER_LEFT_9_PENALTY=2.152833
PRIMER_RIGHT_9_PENALTY=5.609449
PRIMER_LEFT_9_SEQUENCE=TCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_9_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_9=818,21
PRIMER_RIGHT_9=1758,23
PRIMER_LEFT_9_TM=58.847
PRIMER_RIGHT_9_TM=57.391
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.1000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=941
PRIMER_PAIR_10_PENALTY=7.797818
PRIMER_LEFT_10_PENALTY=2.152833
PRIMER_RIGHT_10_PENALTY=5.644986
PRIMER_LEFT_10_SEQUENCE=CCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_10_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_10=819,21
PRIMER_RIGHT_10=1759,24
PRIMER_LEFT_10_TM=58.847
PRIMER_RIGHT_10_TM=58.355
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=41.667
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=941
PRIMER_PAIR_11_PENALTY=7.797818
PRIMER_LEFT_11_PENALTY=2.152833
PRIMER_RIGHT_11_PENALTY=5.644986
PRIMER_LEFT_11_SEQUENCE=TCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_11_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_11=818,21
PRIMER_RIGHT_11=1759,24
PRIMER_LEFT_11_TM=58.847
PRIMER_RIGHT_11_TM=58.355
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=41.667
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=942
PRIMER_PAIR_12_PENALTY=7.815617
PRIMER_LEFT_12_PENALTY=2.152833
PRIMER_RIGHT_12_PENALTY=5.662784
PRIMER_LEFT_12_SEQUENCE=CCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_12_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_12=819,21
PRIMER_RIGHT_12=1758,22
PRIMER_LEFT_12_TM=58.847
PRIMER_RIGHT_12_TM=56.337
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=940
PRIMER_PAIR_13_PENALTY=7.815617
PRIMER_LEFT_13_PENALTY=2.152833
PRIMER_RIGHT_13_PENALTY=5.662784
PRIMER_LEFT_13_SEQUENCE=TCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_13_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_13=818,21
PRIMER_RIGHT_13=1758,22
PRIMER_LEFT_13_TM=58.847
PRIMER_RIGHT_13_TM=56.337
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=941
PRIMER_PAIR_14_PENALTY=7.826037
PRIMER_LEFT_14_PENALTY=2.216588
PRIMER_RIGHT_14_PENALTY=5.609449
PRIMER_LEFT_14_SEQUENCE=CCGTTCAATGCCTTAACTCTCT
PRIMER_RIGHT_14_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_14=819,22
PRIMER_RIGHT_14=1758,23
PRIMER_LEFT_14_TM=59.783
PRIMER_RIGHT_14_TM=57.391
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=940
PRIMER_PAIR_15_PENALTY=7.826037
PRIMER_LEFT_15_PENALTY=2.216588
PRIMER_RIGHT_15_PENALTY=5.609449
PRIMER_LEFT_15_SEQUENCE=CTCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_15_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_15=817,22
PRIMER_RIGHT_15=1758,23
PRIMER_LEFT_15_TM=59.783
PRIMER_RIGHT_15_TM=57.391
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=942
PRIMER_PAIR_16_PENALTY=7.861573
PRIMER_LEFT_16_PENALTY=2.216588
PRIMER_RIGHT_16_PENALTY=5.644986
PRIMER_LEFT_16_SEQUENCE=CCGTTCAATGCCTTAACTCTCT
PRIMER_RIGHT_16_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_16=819,22
PRIMER_RIGHT_16=1759,24
PRIMER_LEFT_16_TM=59.783
PRIMER_RIGHT_16_TM=58.355
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=941
PRIMER_PAIR_17_PENALTY=7.861573
PRIMER_LEFT_17_PENALTY=2.216588
PRIMER_RIGHT_17_PENALTY=5.644986
PRIMER_LEFT_17_SEQUENCE=CTCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_17_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_17=817,22
PRIMER_RIGHT_17=1759,24
PRIMER_LEFT_17_TM=59.783
PRIMER_RIGHT_17_TM=58.355
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=41.667
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.1000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=943
PRIMER_PAIR_18_PENALTY=7.879371
PRIMER_LEFT_18_PENALTY=2.216588
PRIMER_RIGHT_18_PENALTY=5.662784
PRIMER_LEFT_18_SEQUENCE=CCGTTCAATGCCTTAACTCTCT
PRIMER_RIGHT_18_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_18=819,22
PRIMER_RIGHT_18=1758,22
PRIMER_LEFT_18_TM=59.783
PRIMER_RIGHT_18_TM=56.337
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=940
PRIMER_PAIR_19_PENALTY=7.879371
PRIMER_LEFT_19_PENALTY=2.216588
PRIMER_RIGHT_19_PENALTY=5.662784
PRIMER_LEFT_19_SEQUENCE=CTCCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_19_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_19=817,22
PRIMER_RIGHT_19=1758,22
PRIMER_LEFT_19_TM=59.783
PRIMER_RIGHT_19_TM=56.337
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.1000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=942
PRIMER_PAIR_20_PENALTY=7.911514
PRIMER_LEFT_20_PENALTY=2.476351
PRIMER_RIGHT_20_PENALTY=5.435163
PRIMER_LEFT_20_SEQUENCE=TGAGACACACCAATCACAACA
PRIMER_RIGHT_20_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_20=928,21
PRIMER_RIGHT_20=1760,25
PRIMER_LEFT_20_TM=58.524
PRIMER_RIGHT_20_TM=59.565
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=833
PRIMER_PAIR_21_PENALTY=7.942553
PRIMER_LEFT_21_PENALTY=2.507391
PRIMER_RIGHT_21_PENALTY=5.435163
PRIMER_LEFT_21_SEQUENCE=GTGAGACACACCAATCACAACA
PRIMER_RIGHT_21_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_21=927,22
PRIMER_RIGHT_21=1760,25
PRIMER_LEFT_21_TM=59.493
PRIMER_RIGHT_21_TM=59.565
PRIMER_LEFT_21_GC_PERCENT=45.455
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=7.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=834
PRIMER_PAIR_22_PENALTY=8.047333
PRIMER_LEFT_22_PENALTY=2.612170
PRIMER_RIGHT_22_PENALTY=5.435163
PRIMER_LEFT_22_SEQUENCE=TCCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_22_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_22=818,22
PRIMER_RIGHT_22=1760,25
PRIMER_LEFT_22_TM=60.612
PRIMER_RIGHT_22_TM=59.565
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=943
PRIMER_PAIR_23_PENALTY=8.085800
PRIMER_LEFT_23_PENALTY=2.476351
PRIMER_RIGHT_23_PENALTY=5.609449
PRIMER_LEFT_23_SEQUENCE=TGAGACACACCAATCACAACA
PRIMER_RIGHT_23_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_23=928,21
PRIMER_RIGHT_23=1758,23
PRIMER_LEFT_23_TM=58.524
PRIMER_RIGHT_23_TM=57.391
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=831
PRIMER_PAIR_24_PENALTY=8.116840
PRIMER_LEFT_24_PENALTY=2.507391
PRIMER_RIGHT_24_PENALTY=5.609449
PRIMER_LEFT_24_SEQUENCE=GTGAGACACACCAATCACAACA
PRIMER_RIGHT_24_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_24=927,22
PRIMER_RIGHT_24=1758,23
PRIMER_LEFT_24_TM=59.493
PRIMER_RIGHT_24_TM=57.391
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=43.478
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=7.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=832
PRIMER_PAIR_25_PENALTY=8.121337
PRIMER_LEFT_25_PENALTY=2.476351
PRIMER_RIGHT_25_PENALTY=5.644986
PRIMER_LEFT_25_SEQUENCE=TGAGACACACCAATCACAACA
PRIMER_RIGHT_25_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_25=928,21
PRIMER_RIGHT_25=1759,24
PRIMER_LEFT_25_TM=58.524
PRIMER_RIGHT_25_TM=58.355
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=41.667
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=832
PRIMER_PAIR_26_PENALTY=8.139135
PRIMER_LEFT_26_PENALTY=2.476351
PRIMER_RIGHT_26_PENALTY=5.662784
PRIMER_LEFT_26_SEQUENCE=TGAGACACACCAATCACAACA
PRIMER_RIGHT_26_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_26=928,21
PRIMER_RIGHT_26=1758,22
PRIMER_LEFT_26_TM=58.524
PRIMER_RIGHT_26_TM=56.337
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=831
PRIMER_PAIR_27_PENALTY=8.152376
PRIMER_LEFT_27_PENALTY=2.507391
PRIMER_RIGHT_27_PENALTY=5.644986
PRIMER_LEFT_27_SEQUENCE=GTGAGACACACCAATCACAACA
PRIMER_RIGHT_27_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_27=927,22
PRIMER_RIGHT_27=1759,24
PRIMER_LEFT_27_TM=59.493
PRIMER_RIGHT_27_TM=58.355
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=41.667
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=833
PRIMER_PAIR_28_PENALTY=8.170175
PRIMER_LEFT_28_PENALTY=2.507391
PRIMER_RIGHT_28_PENALTY=5.662784
PRIMER_LEFT_28_SEQUENCE=GTGAGACACACCAATCACAACA
PRIMER_RIGHT_28_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_28=927,22
PRIMER_RIGHT_28=1758,22
PRIMER_LEFT_28_TM=59.493
PRIMER_RIGHT_28_TM=56.337
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=832
PRIMER_PAIR_29_PENALTY=8.221619
PRIMER_LEFT_29_PENALTY=2.612170
PRIMER_RIGHT_29_PENALTY=5.609449
PRIMER_LEFT_29_SEQUENCE=TCCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_29_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_29=818,22
PRIMER_RIGHT_29=1758,23
PRIMER_LEFT_29_TM=60.612
PRIMER_RIGHT_29_TM=57.391
PRIMER_LEFT_29_GC_PERCENT=45.455
PRIMER_RIGHT_29_GC_PERCENT=43.478
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=941
PRIMER_PAIR_30_PENALTY=8.257156
PRIMER_LEFT_30_PENALTY=2.612170
PRIMER_RIGHT_30_PENALTY=5.644986
PRIMER_LEFT_30_SEQUENCE=TCCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_30_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_30=818,22
PRIMER_RIGHT_30=1759,24
PRIMER_LEFT_30_TM=60.612
PRIMER_RIGHT_30_TM=58.355
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=41.667
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=942
PRIMER_PAIR_31_PENALTY=8.274954
PRIMER_LEFT_31_PENALTY=2.612170
PRIMER_RIGHT_31_PENALTY=5.662784
PRIMER_LEFT_31_SEQUENCE=TCCGTTCAATGCCTTAACTCTC
PRIMER_RIGHT_31_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_31=818,22
PRIMER_RIGHT_31=1758,22
PRIMER_LEFT_31_TM=60.612
PRIMER_RIGHT_31_TM=56.337
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=941
PRIMER_PAIR_32_PENALTY=8.460996
PRIMER_LEFT_32_PENALTY=3.025833
PRIMER_RIGHT_32_PENALTY=5.435163
PRIMER_LEFT_32_SEQUENCE=CCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_32_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_32=819,20
PRIMER_RIGHT_32=1760,25
PRIMER_LEFT_32_TM=56.974
PRIMER_RIGHT_32_TM=59.565
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.1000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=942
PRIMER_PAIR_33_PENALTY=8.635282
PRIMER_LEFT_33_PENALTY=3.025833
PRIMER_RIGHT_33_PENALTY=5.609449
PRIMER_LEFT_33_SEQUENCE=CCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_33_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_33=819,20
PRIMER_RIGHT_33=1758,23
PRIMER_LEFT_33_TM=56.974
PRIMER_RIGHT_33_TM=57.391
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=43.478
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.1000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=940
PRIMER_PAIR_34_PENALTY=8.670819
PRIMER_LEFT_34_PENALTY=3.025833
PRIMER_RIGHT_34_PENALTY=5.644986
PRIMER_LEFT_34_SEQUENCE=CCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_34_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_34=819,20
PRIMER_RIGHT_34=1759,24
PRIMER_LEFT_34_TM=56.974
PRIMER_RIGHT_34_TM=58.355
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=41.667
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.1000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=941
PRIMER_PAIR_35_PENALTY=8.688617
PRIMER_LEFT_35_PENALTY=3.025833
PRIMER_RIGHT_35_PENALTY=5.662784
PRIMER_LEFT_35_SEQUENCE=CCGTTCAATGCCTTAACTCT
PRIMER_RIGHT_35_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_35=819,20
PRIMER_RIGHT_35=1758,22
PRIMER_LEFT_35_TM=56.974
PRIMER_RIGHT_35_TM=56.337
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.1000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=940
PRIMER_PAIR_36_PENALTY=8.878747
PRIMER_LEFT_36_PENALTY=3.443584
PRIMER_RIGHT_36_PENALTY=5.435163
PRIMER_LEFT_36_SEQUENCE=TGCCTTAACTCTCTCGATCAACT
PRIMER_RIGHT_36_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_36=827,23
PRIMER_RIGHT_36=1760,25
PRIMER_LEFT_36_TM=59.556
PRIMER_RIGHT_36_TM=59.565
PRIMER_LEFT_36_GC_PERCENT=43.478
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=934
PRIMER_PAIR_37_PENALTY=8.951857
PRIMER_LEFT_37_PENALTY=3.516694
PRIMER_RIGHT_37_PENALTY=5.435163
PRIMER_LEFT_37_SEQUENCE=AGTGAGACACACCAATCACAACA
PRIMER_RIGHT_37_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_37=926,23
PRIMER_RIGHT_37=1760,25
PRIMER_LEFT_37_TM=60.517
PRIMER_RIGHT_37_TM=59.565
PRIMER_LEFT_37_GC_PERCENT=43.478
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=8.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=835
PRIMER_PAIR_38_PENALTY=9.053033
PRIMER_LEFT_38_PENALTY=3.443584
PRIMER_RIGHT_38_PENALTY=5.609449
PRIMER_LEFT_38_SEQUENCE=TGCCTTAACTCTCTCGATCAACT
PRIMER_RIGHT_38_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_38=827,23
PRIMER_RIGHT_38=1758,23
PRIMER_LEFT_38_TM=59.556
PRIMER_RIGHT_38_TM=57.391
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=43.478
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=932
PRIMER_PAIR_39_PENALTY=9.088570
PRIMER_LEFT_39_PENALTY=3.443584
PRIMER_RIGHT_39_PENALTY=5.644986
PRIMER_LEFT_39_SEQUENCE=TGCCTTAACTCTCTCGATCAACT
PRIMER_RIGHT_39_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_39=827,23
PRIMER_RIGHT_39=1759,24
PRIMER_LEFT_39_TM=59.556
PRIMER_RIGHT_39_TM=58.355
PRIMER_LEFT_39_GC_PERCENT=43.478
PRIMER_RIGHT_39_GC_PERCENT=41.667
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=933
PRIMER_PAIR_40_PENALTY=9.106368
PRIMER_LEFT_40_PENALTY=3.443584
PRIMER_RIGHT_40_PENALTY=5.662784
PRIMER_LEFT_40_SEQUENCE=TGCCTTAACTCTCTCGATCAACT
PRIMER_RIGHT_40_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_40=827,23
PRIMER_RIGHT_40=1758,22
PRIMER_LEFT_40_TM=59.556
PRIMER_RIGHT_40_TM=56.337
PRIMER_LEFT_40_GC_PERCENT=43.478
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=932
PRIMER_PAIR_41_PENALTY=9.126144
PRIMER_LEFT_41_PENALTY=3.516694
PRIMER_RIGHT_41_PENALTY=5.609449
PRIMER_LEFT_41_SEQUENCE=AGTGAGACACACCAATCACAACA
PRIMER_RIGHT_41_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_41=926,23
PRIMER_RIGHT_41=1758,23
PRIMER_LEFT_41_TM=60.517
PRIMER_RIGHT_41_TM=57.391
PRIMER_LEFT_41_GC_PERCENT=43.478
PRIMER_RIGHT_41_GC_PERCENT=43.478
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=8.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=833
PRIMER_PAIR_42_PENALTY=9.161680
PRIMER_LEFT_42_PENALTY=3.516694
PRIMER_RIGHT_42_PENALTY=5.644986
PRIMER_LEFT_42_SEQUENCE=AGTGAGACACACCAATCACAACA
PRIMER_RIGHT_42_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_42=926,23
PRIMER_RIGHT_42=1759,24
PRIMER_LEFT_42_TM=60.517
PRIMER_RIGHT_42_TM=58.355
PRIMER_LEFT_42_GC_PERCENT=43.478
PRIMER_RIGHT_42_GC_PERCENT=41.667
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=8.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=834
PRIMER_PAIR_43_PENALTY=9.179478
PRIMER_LEFT_43_PENALTY=3.516694
PRIMER_RIGHT_43_PENALTY=5.662784
PRIMER_LEFT_43_SEQUENCE=AGTGAGACACACCAATCACAACA
PRIMER_RIGHT_43_SEQUENCE=GCAAAGTCTCACTTAGAAACCA
PRIMER_LEFT_43=926,23
PRIMER_RIGHT_43=1758,22
PRIMER_LEFT_43_TM=60.517
PRIMER_RIGHT_43_TM=56.337
PRIMER_LEFT_43_GC_PERCENT=43.478
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=8.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=833
PRIMER_PAIR_44_PENALTY=9.285735
PRIMER_LEFT_44_PENALTY=3.850573
PRIMER_RIGHT_44_PENALTY=5.435163
PRIMER_LEFT_44_SEQUENCE=CGTTCAATGCCTTAACTCTCTC
PRIMER_RIGHT_44_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_44=820,22
PRIMER_RIGHT_44=1760,25
PRIMER_LEFT_44_TM=58.149
PRIMER_RIGHT_44_TM=59.565
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=941
PRIMER_PAIR_45_PENALTY=9.335881
PRIMER_LEFT_45_PENALTY=3.900718
PRIMER_RIGHT_45_PENALTY=5.435163
PRIMER_LEFT_45_SEQUENCE=GTGAGACACACCAATCACAACAA
PRIMER_RIGHT_45_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_45=927,23
PRIMER_RIGHT_45=1760,25
PRIMER_LEFT_45_TM=60.901
PRIMER_RIGHT_45_TM=59.565
PRIMER_LEFT_45_GC_PERCENT=43.478
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=834
PRIMER_PAIR_46_PENALTY=9.436034
PRIMER_LEFT_46_PENALTY=4.000871
PRIMER_RIGHT_46_PENALTY=5.435163
PRIMER_LEFT_46_SEQUENCE=CATCTTCATGTCATCACCAGTC
PRIMER_RIGHT_46_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_46=787,22
PRIMER_RIGHT_46=1760,25
PRIMER_LEFT_46_TM=57.999
PRIMER_RIGHT_46_TM=59.565
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=974
PRIMER_PAIR_47_PENALTY=9.460022
PRIMER_LEFT_47_PENALTY=3.850573
PRIMER_RIGHT_47_PENALTY=5.609449
PRIMER_LEFT_47_SEQUENCE=CGTTCAATGCCTTAACTCTCTC
PRIMER_RIGHT_47_SEQUENCE=GCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_47=820,22
PRIMER_RIGHT_47=1758,23
PRIMER_LEFT_47_TM=58.149
PRIMER_RIGHT_47_TM=57.391
PRIMER_LEFT_47_GC_PERCENT=45.455
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=939
PRIMER_PAIR_48_PENALTY=9.485800
PRIMER_LEFT_48_PENALTY=4.050637
PRIMER_RIGHT_48_PENALTY=5.435163
PRIMER_LEFT_48_SEQUENCE=GAGACACACCAATCACAACAA
PRIMER_RIGHT_48_SEQUENCE=AAGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_48=929,21
PRIMER_RIGHT_48=1760,25
PRIMER_LEFT_48_TM=56.949
PRIMER_RIGHT_48_TM=59.565
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=832
PRIMER_PAIR_49_PENALTY=9.495558
PRIMER_LEFT_49_PENALTY=3.850573
PRIMER_RIGHT_49_PENALTY=5.644986
PRIMER_LEFT_49_SEQUENCE=CGTTCAATGCCTTAACTCTCTC
PRIMER_RIGHT_49_SEQUENCE=AGCAAAGTCTCACTTAGAAACCAG
PRIMER_LEFT_49=820,22
PRIMER_RIGHT_49=1759,24
PRIMER_LEFT_49_TM=58.149
PRIMER_RIGHT_49_TM=58.355
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=41.667
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=940
=
SEQUENCE_ID=MAGIC.287_3_2737292_2737780_bur-0_oy-0_561889_4840_TRUE
SEQUENCE_TARGET=950,588
SEQUENCE_TEMPLATE=CTCCTGTAAGAATTGCCAGAAAGAGTGTCAAGGCCTAACATACAGGGTAGTATCTTTGCAGAGCGAAGAATCTAAACGAGNCAGCTANANAGAATTATAAGTAGAATTGCCGATTCCGGAAACAACTTGAACTTACCATGAATTTCTTCTGCTCTTCCAATGACGGTTGATACTGTTCTTGAAATACACTGAGGCCATTAATCTAACGTCCACATGGGACACCAAATCCTTGGATGCAATTACTTCCTGCAACCAAACCAGAGGAAATGAGCGAGTAAAATCAAGCTGACATTTGCAGAAACCACAAGACACATAGATTAGAAANGCTACCATAAGGCAAGAACAGAAACCAGGTCTACTCTCCGACNGAGATAGAGCCGCCTCCGCCGGCCGCCGAACGGTCTCGTCACCGCTCATTGAATTAGCTAATAGCGTGTACATCGCCGGCAAATCGGAAGCCGATAACGCCATCAGATCCGACGATATTGTTGACCTAATTAACCAAGGAAACGTCGAGGATGAACCCTAGAGGTTTGTTACGACGACGATAAAATCTCACGGCGAAGGAAGGAAAACGCGAAGCTAGTAGAGACAGAGAAGAGAAGAAAGATCGTCTTCTTCTTCNTCCGTCTTTCAACTTCTCTTAAGCTCTTGAAACGTAACAATGTNACTAGTGAAAATGATTTCNTNTTTTTTTTTTAACAGTCGAAGAAAAAANAAATACTTACCGTCGCTCTGATCGGATTCCGATCACCCAACCGGAGNCTTTCGTTTTGGGCTAACTACAGATTATTGGGCNTATAATAAATAAGCCCAAATTAATAATNGCGCGTGATCTTAACGGCGCGTGAACGNAAATAAACAACGATCGNTCGTGTTTGCTAAAATCTTATTGGTGGAGATACGTGGGATTGCATCAAATTTGCCACGTATGATACCCGCCTTAAATTGAGAACGGGAAGCAACCGGAATTTATTTTCAGGAAATACGAAACAACCCCNGCTAGTTTTTGAAGTGANTAATTGCCCCTACCAATTTCTTAATCCAATTGACAATTTTGGTGTCTTTTGTAAGACTATTAAATATGGGGAATACTCTAAATTAATTAAAATATATATGTCANTCGAGAAATTTCCTTTNTTTCCTTTAAACCCTGCGAACCTATTTAATGATGTCCTTCTCGATTTTTCTCGAAGCGNTGAGTTTCGTGAATAAGACACGCTTAGAAGAGGAAGAAGAAGATTCGTTGACAACAAAACAATAAAAGAGAAAAAGATTGAGAGGTTGTACTTTCGGGATGGTGAGAACAAGATTGGCGATATCTGTTGTTCTTGTGTCGACATTGTTGTTGTTGAATGTGAAAGCGAAGAGCGTTGATCCTTACAAGGTACGNTTTGTGATTCATCTTGTTTTCTTTCCATTCACAAGTCTTTTGATTGGGGCCGGAAATAATGATTACTTTTGATTGTCCCCTTTCTCATTGAAGNTNGNNCTGNNNNNNTTNNGNGTNTTANNGAANNNANNNATTTTNNNTANNNNANNNTNTGCTNANTNNNNNTTNNTATTGNNNANNANNNNTATNNACTNNNGNNNNNANNTTNNGGNTNTTNNAGNANNNAAGGATNNNNNNNNNNNTGANATNNANNANGNTTTNCNCNAGTANNNNNNNNTNNTTNCTANTNGNTNATNAANNTNATNANNNNAATGANNATNNANNNNNAGNTTNNTNNNGTANCTNNNGATNNNNNNNNGCNNGNAATCNCNGAAANNTNNTCCANANNNAANCANNNNNAANGNTGNTNANNANAANTTNNNTGNGATNAANAANNNTAGNTNNCNNATNNNCCTNNGNGATNTNTNNTGNNANNNAATCTNTNANNATATNANAAGATNANNNNNNNNNTNTGNNCANGNGNNNNTNGCNNANATTNAATNNNNGTNGNTNTNNNNNNNNCAANTTTNNNCNNTNNNNNATGTGTNNNNNNNNNTGNNATCNNATCTNNNGNANANNANANGANNANNNNTGNTCNNTATGGNNATGNNNNAGNACANNNTGNATNTGNNTCNGGNTTNCCCNNANNTANNNGNGNGTANTCTTNCTNNTNAAGNGNNGNNNGTNTNANCTTNGGANNGNCNGGNNNNTGNNNNNATNNGGGNNNTNNNGGAGGNNNNNNNNNGTNTTCTTNCTCNTNNNGNGGNNNTNGTGNNNNCNNCTTTGGNNNNGGTNTGGNNGNNNNNTTNNCNANNTNTNNGGGCGGTNGNNNNNNNGGNANGNNNCANNTNGNTNNCTTTNNNNNNNNNTCGNNTGNNGNNTCNNANTNAAAGNNCNNTNNTGNNNNNGNTATNNANNNNATNNNNTCNCANGTNTNTNNGNNGGNNGTTNTNNANNAANGGANNANNTGGCTTTNGTNNTCGNATNNTNNATNTCANAGNGNANNNCNGTANCANNNATNCANCATANNNNAANTNNCNGNGTNGTNNNAAGGAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_16804349_16804839_ws-0_ler-0_211051_391656_TRUE
SEQUENCE_TARGET=950,590
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_10480482_10480518_oy-0_zu-0_4643_1219_TRUE
SEQUENCE_TARGET=950,136
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_5929114_5929209_bur-0_mt-0_5564_5255_TRUE
SEQUENCE_TARGET=950,195
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNATTAANNTNGAGNNNNTNATACTNGCNNGATGCNNAAGTTTGNNGGAGCTCCCTTCCTTTATTGGAAAAGNCACTNATCTCANGATTCTNNATNTTNGANATNTCNNATGTCTGGTNGAGTTACCNTCCTTTATNGGAAACCTTCATAAGTTGNCTNAANTNAGNCTGANAGGATGCNAAAAGCTACAAGTTCTTCCGACNAATATCAACTTGGAATTTCTTNATGAACTTGATCTCACAGATTGCATCTTGTTGAAAACATTTCCNNNGATCTCNACAAANATTNAACGTCTNNATCTTANGGGGACTCNNATAGAAGAAGTTNCTTNGTCCNTCANGTCATGGCCTCGCCTTNNGNATTTGCANATGNTATACAGNNAAAACCTNAGTGAATTCTCACATGTTCTTGAGANGATCACNGTGCTNGAATTGAGNGATATAAATATACGNGAAATGACNCCATGGCTCAATAGAATCNCTCNTCTACGTCGATTNANACTCAGNGGATGCNGAAAACTNGTATCACTCCCACAACTTNCNGATTCCCTAANAATTCTNGATGCAGANAATTGTGNCTCTCTTGAGAGACTAGNTTGCTCTTTTAACAATCCAAATATTNNANNTCTCGACTTTACCAACTGNCTCAAACTAGATAAAGAAGCAAGAGANNTTATCATTCAAGCAACGGCTCGNNATTATTCNATCTTACCNAGTAGAGAAGTTCATGAGTACATCANTAANCGAGCCATTGGAANTTCTCTTANNNTGAAGTTGAATNAAAGGGNNNTTCCTACATCCATGANATTTAAGGCTTGCATNGTTTTGGCTGNTAANNNTGNNCNTGAAGCTNNTANNNNNNNNNNNNNNNNNNNNNNNNTGACCNTNATGGAANGGCAGAATGATTTNATCNCTTCTACGTNTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGAGCATNTGTACNCANTCGAAGTTNNAGTTGANGTTGAGGTNACTTCNGATGAGCTTGTCTTTGATTTCNAGCTCAACANTGAGAAATGGGAGATAGNAGAATGTGGGGTACNNGAANTGAAGAANCATGTTCNAANNTTTTCNGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACANTATGAACGTCTNGGCNAGAGCNTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNANACATATCTCTACAGGCNAAAACNAACAATNGAANTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTATTNAAGNANGTGAGTCCACTTGTGGAAAAGGAAAANNNNNNNNNNNNNNNNNNNGAAATTACTTGGATNNTGCATTTANNTGAAGCNACTCTAGGAAGNATCTGAAGTTGCTTGGAAACTNCAAGCACATCGTNATCGTATGGTGTATNGTAGTATNATATCATTTGNTCAANATTCNGCATTNTCTCNA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14374534_14374919_can-0_tsu-0_29350_78433_TRUE
SEQUENCE_TARGET=950,485
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_2_6729453_6729990_mt-0_zu-0_10393_718107_TRUE
SEQUENCE_TARGET=950,637
SEQUENCE_TEMPLATE=AGCCNGACAAACTTGAGAGGGTAAAAANGTAAAANCGCAGGGGAGACCCTAGGTCAGCTCCTANCCTATAAATAGCAGAAGACACNATTGGGANAAGGGANCANGAGTTATTACCTAGAGAATTTAAGAGAGAATAGTTGCCTANCTCTGTTATNTCTTGCTTTNNCGTTNTGTANTTTCTGGTTTTCCGANTTNTACNTNCGGTTCTCCNTTCNGTACTTACGGTTCTNCNTTCTGTTAAATTACCGTCTTTTCCGTTNGTCATTAATAAAGAAGCTACTCCGTTCTCCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_16645770_16645889_can-0_ws-0_19877_28244_TRUE
SEQUENCE_TARGET=950,219
SEQUENCE_TEMPLATE=NGAGAAGAATGAAGAAGANGNAGAAANAAGATATTTTTAGAGTGTGTTTGGGTTCGTNGAGCANTCTGCGTACGGTTTTAAAGGTGAAATGTTACGTTTATTTTATTTTATTTTTCGCAACTTCGATGTTATAATATTTTAGACTTTTAGTAGAAAGTATTTATTTTTAATATTAATTTAATAATTAAANTATTGATNTTTTTGTACTTTATNATTAGAATGACAAATAGNGAGAAAGTACGAAAGAGACACGTACGGTGAAGGTGTNGAAGCGGTGATGAATTTACGATGTGCTCCTTCTTGTTTGTGTTATATAAAAGTCGACAAGGAATTAACTTTTTATATAATGCAAAATTGTTCTCCATGGGATATTTTTCTTGAATGTGATGATTACAATATGCTCAACTAGGATGATTACTTCATTAGTTGTATTTAGTGGATGGTTTTGGCCAGTAGTTTCTTCCAGGCATGAAAGTTCATGCTTCCGTTANNTTTTTTTTTTTTNTTTNCTTATTAGAATAGTATATAGACGACCACAAGNTTTTTTTTAACGAAAAATATGNATTTTTTTNGTAAAGTTNTATTTTTCAACTATTGNTTATGTTCGAAGATGTAAAGAGAGCGATAGCCAATTAGCGGCCAGTAGCTTATTTGTCTCTCAGTGTTTGGTAATGGAATCAGGAAGAGAATAGAATAGTTAGAACNNAATATCNGAAATTACATATNTTTTATTTTGGTCATTACTTTTTAGTTTTTGTAATGACGNTAAATCAAAATTGTGATTTGGAATAAATATATATAATTAGCAAAATCCAGTGGAATGAAGAAAATATTCTATCATACCATATANTATAACCTGCCGTAATTAAAGTCNAAATATTAATAAATAAACATTAAATAATAATGGGAAACTGGAAACTGTTGTACGACACATGACAACATNAAATGAGTGAAAGTNTCGTACAAACTANGGCAGTGGATATTCGGATCTTCAATTGAANTTTNAAATTCGAAAGTGTGACTTGTGANTTGTGANTAGTACGGCTCATTATCTTTGCTAGAAGCTTCCAACTCGGTTTGATTTTCGGTTCAGACGAAAGACCTTTTTTGACATAAAAANNGTCAGATGAAAGTAGTTGCTTGAGAGTTTTGTTATCATGTTATGAAGCAGTNACNAGTGAGCCAAAGTTGCTATATTGTATTGATATTCTGTTAGNANCATATTNCAAATCTACGGNNNAAANAAAAAAAAAAAAAAANTNGAAAGAGTTGGAAAAAAGTTCTAGTTACAAATATTTCTCTAATTACNTCTCGGNACTGATACCGACTAGTGCATACCACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAATGTTTACCNGCTGAGTTACTTCATCNAAANNTGGTCGTATACATTGCGCGCGCAATTACATGCAACATGTCTTTGACCGTAAATATCACATTAAAATAGAATGACAGTCACTTTCTGATATAATATATTAATATACACCAACCAGCATGACTAGGATCAAGGTGTACAACAANTTTTTTTTTGAATTTATCAAATTCACACAAAACATATCGTGTACAACTCTAACGTACGTTTCACATCGGCTAAGCCTTGTATTTCTTTATTTGTAGCTTGTACGGAACATTTATANCTTGCAACACTGACAATTTTGCAGATTAGTTGCTTTGTTCTGATAAAGATGGTTTAAGAATTTAGTTATTCNGCGGTTTACTTTCGTATGGAATACAGCAGATACCACAATTTAAGGTACCAACTTGGAAAGAGTCATTCGGATCATCGGATGAGACAACTATTTTCATCGACCAAGTAAAGAATATTTCATCGATCGGTTTANTTTTGTTAAACATAATTGCAGTATATGTGTGTTAACCGTCAAAAATTAGAGTGCCAATAAATNTTTTTTTACTNTACCAATNAAAAAANNAAAAAAATAACTGGAAGTGAAGAAAATATCATTNGCAAAATAAATTAAATAAAACGTAACGTACGCTAATATGGAGGAGGAATTTCGGAATTTCATCAGTTCTTTTTTTGAAGCTTATCGACAACGCCTGACGGCTTTGCTGGATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.450803
PRIMER_LEFT_0_PENALTY=0.611852
PRIMER_RIGHT_0_PENALTY=0.838952
PRIMER_LEFT_0_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_0_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_0=635,20
PRIMER_RIGHT_0=1544,20
PRIMER_LEFT_0_TM=59.388
PRIMER_RIGHT_0_TM=59.161
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=910
PRIMER_PAIR_1_PENALTY=1.654942
PRIMER_LEFT_1_PENALTY=0.611852
PRIMER_RIGHT_1_PENALTY=1.043090
PRIMER_LEFT_1_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_1_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_1=635,20
PRIMER_RIGHT_1=1544,21
PRIMER_LEFT_1_TM=59.388
PRIMER_RIGHT_1_TM=60.043
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=910
PRIMER_PAIR_2_PENALTY=2.047098
PRIMER_LEFT_2_PENALTY=0.611852
PRIMER_RIGHT_2_PENALTY=1.435246
PRIMER_LEFT_2_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_2_SEQUENCE=GATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_2=635,20
PRIMER_RIGHT_2=1547,21
PRIMER_LEFT_2_TM=59.388
PRIMER_RIGHT_2_TM=59.565
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=913
PRIMER_PAIR_3_PENALTY=2.128687
PRIMER_LEFT_3_PENALTY=1.289736
PRIMER_RIGHT_3_PENALTY=0.838952
PRIMER_LEFT_3_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_3_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_3=634,21
PRIMER_RIGHT_3=1544,20
PRIMER_LEFT_3_TM=60.290
PRIMER_RIGHT_3_TM=59.161
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=911
PRIMER_PAIR_4_PENALTY=2.332826
PRIMER_LEFT_4_PENALTY=1.289736
PRIMER_RIGHT_4_PENALTY=1.043090
PRIMER_LEFT_4_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_4_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_4=634,21
PRIMER_RIGHT_4=1544,21
PRIMER_LEFT_4_TM=60.290
PRIMER_RIGHT_4_TM=60.043
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=911
PRIMER_PAIR_5_PENALTY=2.453689
PRIMER_LEFT_5_PENALTY=0.611852
PRIMER_RIGHT_5_PENALTY=1.841837
PRIMER_LEFT_5_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_5_SEQUENCE=TCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_5=635,20
PRIMER_RIGHT_5=1545,20
PRIMER_LEFT_5_TM=59.388
PRIMER_RIGHT_5_TM=58.158
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=911
PRIMER_PAIR_6_PENALTY=2.711878
PRIMER_LEFT_6_PENALTY=0.611852
PRIMER_RIGHT_6_PENALTY=2.100026
PRIMER_LEFT_6_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_6_SEQUENCE=TCCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_6=635,20
PRIMER_RIGHT_6=1545,21
PRIMER_LEFT_6_TM=59.388
PRIMER_RIGHT_6_TM=61.100
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=911
PRIMER_PAIR_7_PENALTY=2.724982
PRIMER_LEFT_7_PENALTY=1.289736
PRIMER_RIGHT_7_PENALTY=1.435246
PRIMER_LEFT_7_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_7_SEQUENCE=GATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_7=634,21
PRIMER_RIGHT_7=1547,21
PRIMER_LEFT_7_TM=60.290
PRIMER_RIGHT_7_TM=59.565
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=914
PRIMER_PAIR_8_PENALTY=3.002271
PRIMER_LEFT_8_PENALTY=0.611852
PRIMER_RIGHT_8_PENALTY=2.390419
PRIMER_LEFT_8_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_8_SEQUENCE=ATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_8=635,20
PRIMER_RIGHT_8=1546,20
PRIMER_LEFT_8_TM=59.388
PRIMER_RIGHT_8_TM=57.610
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=912
PRIMER_PAIR_9_PENALTY=3.002373
PRIMER_LEFT_9_PENALTY=0.611852
PRIMER_RIGHT_9_PENALTY=2.390521
PRIMER_LEFT_9_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_9_SEQUENCE=GATCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_9=635,20
PRIMER_RIGHT_9=1547,22
PRIMER_LEFT_9_TM=59.388
PRIMER_RIGHT_9_TM=60.391
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=913
PRIMER_PAIR_10_PENALTY=3.073132
PRIMER_LEFT_10_PENALTY=0.611852
PRIMER_RIGHT_10_PENALTY=2.461281
PRIMER_LEFT_10_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_10_SEQUENCE=ATCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_10=635,20
PRIMER_RIGHT_10=1546,21
PRIMER_LEFT_10_TM=59.388
PRIMER_RIGHT_10_TM=58.539
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=912
PRIMER_PAIR_11_PENALTY=3.115169
PRIMER_LEFT_11_PENALTY=2.276218
PRIMER_RIGHT_11_PENALTY=0.838952
PRIMER_LEFT_11_SEQUENCE=CGGCCAGTAGCTTATTTGTCTC
PRIMER_RIGHT_11_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_11=636,22
PRIMER_RIGHT_11=1544,20
PRIMER_LEFT_11_TM=60.276
PRIMER_RIGHT_11_TM=59.161
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=909
PRIMER_PAIR_12_PENALTY=3.128441
PRIMER_LEFT_12_PENALTY=2.289490
PRIMER_RIGHT_12_PENALTY=0.838952
PRIMER_LEFT_12_SEQUENCE=TAGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_12_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_12=633,22
PRIMER_RIGHT_12=1544,20
PRIMER_LEFT_12_TM=60.289
PRIMER_RIGHT_12_TM=59.161
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=912
PRIMER_PAIR_13_PENALTY=3.131573
PRIMER_LEFT_13_PENALTY=1.289736
PRIMER_RIGHT_13_PENALTY=1.841837
PRIMER_LEFT_13_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_13_SEQUENCE=TCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_13=634,21
PRIMER_RIGHT_13=1545,20
PRIMER_LEFT_13_TM=60.290
PRIMER_RIGHT_13_TM=58.158
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=912
PRIMER_PAIR_14_PENALTY=3.286905
PRIMER_LEFT_14_PENALTY=2.447953
PRIMER_RIGHT_14_PENALTY=0.838952
PRIMER_LEFT_14_SEQUENCE=CGGCCAGTAGCTTATTTGTCT
PRIMER_RIGHT_14_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_14=636,21
PRIMER_RIGHT_14=1544,20
PRIMER_LEFT_14_TM=58.552
PRIMER_RIGHT_14_TM=59.161
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=909
PRIMER_PAIR_15_PENALTY=3.319308
PRIMER_LEFT_15_PENALTY=2.276218
PRIMER_RIGHT_15_PENALTY=1.043090
PRIMER_LEFT_15_SEQUENCE=CGGCCAGTAGCTTATTTGTCTC
PRIMER_RIGHT_15_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_15=636,22
PRIMER_RIGHT_15=1544,21
PRIMER_LEFT_15_TM=60.276
PRIMER_RIGHT_15_TM=60.043
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=909
PRIMER_PAIR_16_PENALTY=3.332580
PRIMER_LEFT_16_PENALTY=2.289490
PRIMER_RIGHT_16_PENALTY=1.043090
PRIMER_LEFT_16_SEQUENCE=TAGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_16_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_16=633,22
PRIMER_RIGHT_16=1544,21
PRIMER_LEFT_16_TM=60.289
PRIMER_RIGHT_16_TM=60.043
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=912
PRIMER_PAIR_17_PENALTY=3.389762
PRIMER_LEFT_17_PENALTY=1.289736
PRIMER_RIGHT_17_PENALTY=2.100026
PRIMER_LEFT_17_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_17_SEQUENCE=TCCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_17=634,21
PRIMER_RIGHT_17=1545,21
PRIMER_LEFT_17_TM=60.290
PRIMER_RIGHT_17_TM=61.100
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=912
PRIMER_PAIR_18_PENALTY=3.491043
PRIMER_LEFT_18_PENALTY=2.447953
PRIMER_RIGHT_18_PENALTY=1.043090
PRIMER_LEFT_18_SEQUENCE=CGGCCAGTAGCTTATTTGTCT
PRIMER_RIGHT_18_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_18=636,21
PRIMER_RIGHT_18=1544,21
PRIMER_LEFT_18_TM=58.552
PRIMER_RIGHT_18_TM=60.043
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=909
PRIMER_PAIR_19_PENALTY=3.579247
PRIMER_LEFT_19_PENALTY=2.740295
PRIMER_RIGHT_19_PENALTY=0.838952
PRIMER_LEFT_19_SEQUENCE=TTAGCGGCCAGTAGCTTATTT
PRIMER_RIGHT_19_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_19=632,21
PRIMER_RIGHT_19=1544,20
PRIMER_LEFT_19_TM=58.260
PRIMER_RIGHT_19_TM=59.161
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.2000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=913
PRIMER_PAIR_20_PENALTY=3.591069
PRIMER_LEFT_20_PENALTY=2.752117
PRIMER_RIGHT_20_PENALTY=0.838952
PRIMER_LEFT_20_SEQUENCE=TTCGAAGATGTAAAGAGAGCGA
PRIMER_RIGHT_20_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_20=603,22
PRIMER_RIGHT_20=1544,20
PRIMER_LEFT_20_TM=59.248
PRIMER_RIGHT_20_TM=59.161
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.9000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=942
PRIMER_PAIR_21_PENALTY=3.680155
PRIMER_LEFT_21_PENALTY=1.289736
PRIMER_RIGHT_21_PENALTY=2.390419
PRIMER_LEFT_21_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_21_SEQUENCE=ATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_21=634,21
PRIMER_RIGHT_21=1546,20
PRIMER_LEFT_21_TM=60.290
PRIMER_RIGHT_21_TM=57.610
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=913
PRIMER_PAIR_22_PENALTY=3.680257
PRIMER_LEFT_22_PENALTY=1.289736
PRIMER_RIGHT_22_PENALTY=2.390521
PRIMER_LEFT_22_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_22_SEQUENCE=GATCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_22=634,21
PRIMER_RIGHT_22=1547,22
PRIMER_LEFT_22_TM=60.290
PRIMER_RIGHT_22_TM=60.391
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=914
PRIMER_PAIR_23_PENALTY=3.711464
PRIMER_LEFT_23_PENALTY=2.276218
PRIMER_RIGHT_23_PENALTY=1.435246
PRIMER_LEFT_23_SEQUENCE=CGGCCAGTAGCTTATTTGTCTC
PRIMER_RIGHT_23_SEQUENCE=GATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_23=636,22
PRIMER_RIGHT_23=1547,21
PRIMER_LEFT_23_TM=60.276
PRIMER_RIGHT_23_TM=59.565
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.1000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=912
PRIMER_PAIR_24_PENALTY=3.724736
PRIMER_LEFT_24_PENALTY=2.289490
PRIMER_RIGHT_24_PENALTY=1.435246
PRIMER_LEFT_24_SEQUENCE=TAGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_24_SEQUENCE=GATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_24=633,22
PRIMER_RIGHT_24=1547,21
PRIMER_LEFT_24_TM=60.289
PRIMER_RIGHT_24_TM=59.565
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=915
PRIMER_PAIR_25_PENALTY=3.751016
PRIMER_LEFT_25_PENALTY=1.289736
PRIMER_RIGHT_25_PENALTY=2.461281
PRIMER_LEFT_25_SEQUENCE=AGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_25_SEQUENCE=ATCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_25=634,21
PRIMER_RIGHT_25=1546,21
PRIMER_LEFT_25_TM=60.290
PRIMER_RIGHT_25_TM=58.539
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=913
PRIMER_PAIR_26_PENALTY=3.783386
PRIMER_LEFT_26_PENALTY=2.740295
PRIMER_RIGHT_26_PENALTY=1.043090
PRIMER_LEFT_26_SEQUENCE=TTAGCGGCCAGTAGCTTATTT
PRIMER_RIGHT_26_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_26=632,21
PRIMER_RIGHT_26=1544,21
PRIMER_LEFT_26_TM=58.260
PRIMER_RIGHT_26_TM=60.043
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.2000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=913
PRIMER_PAIR_27_PENALTY=3.795208
PRIMER_LEFT_27_PENALTY=2.752117
PRIMER_RIGHT_27_PENALTY=1.043090
PRIMER_LEFT_27_SEQUENCE=TTCGAAGATGTAAAGAGAGCGA
PRIMER_RIGHT_27_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_27=603,22
PRIMER_RIGHT_27=1544,21
PRIMER_LEFT_27_TM=59.248
PRIMER_RIGHT_27_TM=60.043
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=942
PRIMER_PAIR_28_PENALTY=3.873606
PRIMER_LEFT_28_PENALTY=3.034654
PRIMER_RIGHT_28_PENALTY=0.838952
PRIMER_LEFT_28_SEQUENCE=GTTCGAAGATGTAAAGAGAGCGA
PRIMER_RIGHT_28_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_28=602,23
PRIMER_RIGHT_28=1544,20
PRIMER_LEFT_28_TM=60.035
PRIMER_RIGHT_28_TM=59.161
PRIMER_LEFT_28_GC_PERCENT=43.478
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.9000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=943
PRIMER_PAIR_29_PENALTY=3.883199
PRIMER_LEFT_29_PENALTY=2.447953
PRIMER_RIGHT_29_PENALTY=1.435246
PRIMER_LEFT_29_SEQUENCE=CGGCCAGTAGCTTATTTGTCT
PRIMER_RIGHT_29_SEQUENCE=GATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_29=636,21
PRIMER_RIGHT_29=1547,21
PRIMER_LEFT_29_TM=58.552
PRIMER_RIGHT_29_TM=59.565
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=912
PRIMER_PAIR_30_PENALTY=3.920611
PRIMER_LEFT_30_PENALTY=3.081659
PRIMER_RIGHT_30_PENALTY=0.838952
PRIMER_LEFT_30_SEQUENCE=TAGCGGCCAGTAGCTTATTT
PRIMER_RIGHT_30_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_30=633,20
PRIMER_RIGHT_30=1544,20
PRIMER_LEFT_30_TM=56.918
PRIMER_RIGHT_30_TM=59.161
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=912
PRIMER_PAIR_31_PENALTY=3.924518
PRIMER_LEFT_31_PENALTY=0.611852
PRIMER_RIGHT_31_PENALTY=3.312666
PRIMER_LEFT_31_SEQUENCE=GCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_31_SEQUENCE=ATCCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_31=635,20
PRIMER_RIGHT_31=1546,22
PRIMER_LEFT_31_TM=59.388
PRIMER_RIGHT_31_TM=61.313
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=912
PRIMER_PAIR_32_PENALTY=3.941655
PRIMER_LEFT_32_PENALTY=3.102704
PRIMER_RIGHT_32_PENALTY=0.838952
PRIMER_LEFT_32_SEQUENCE=GATGTAAAGAGAGCGATAGCCAA
PRIMER_RIGHT_32_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_32=609,23
PRIMER_RIGHT_32=1544,20
PRIMER_LEFT_32_TM=59.897
PRIMER_RIGHT_32_TM=59.161
PRIMER_LEFT_32_GC_PERCENT=43.478
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=10.0000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=936
PRIMER_PAIR_33_PENALTY=3.975211
PRIMER_LEFT_33_PENALTY=3.136259
PRIMER_RIGHT_33_PENALTY=0.838952
PRIMER_LEFT_33_SEQUENCE=TCGAAGATGTAAAGAGAGCGA
PRIMER_RIGHT_33_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_33=604,21
PRIMER_RIGHT_33=1544,20
PRIMER_LEFT_33_TM=57.864
PRIMER_RIGHT_33_TM=59.161
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=941
PRIMER_PAIR_34_PENALTY=4.016518
PRIMER_LEFT_34_PENALTY=3.177567
PRIMER_RIGHT_34_PENALTY=0.838952
PRIMER_LEFT_34_SEQUENCE=TGTAAAGAGAGCGATAGCCAA
PRIMER_RIGHT_34_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_34=611,21
PRIMER_RIGHT_34=1544,20
PRIMER_LEFT_34_TM=57.822
PRIMER_RIGHT_34_TM=59.161
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.0000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=934
PRIMER_PAIR_35_PENALTY=4.077744
PRIMER_LEFT_35_PENALTY=3.034654
PRIMER_RIGHT_35_PENALTY=1.043090
PRIMER_LEFT_35_SEQUENCE=GTTCGAAGATGTAAAGAGAGCGA
PRIMER_RIGHT_35_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_35=602,23
PRIMER_RIGHT_35=1544,21
PRIMER_LEFT_35_TM=60.035
PRIMER_RIGHT_35_TM=60.043
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.9000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=943
PRIMER_PAIR_36_PENALTY=4.118055
PRIMER_LEFT_36_PENALTY=2.276218
PRIMER_RIGHT_36_PENALTY=1.841837
PRIMER_LEFT_36_SEQUENCE=CGGCCAGTAGCTTATTTGTCTC
PRIMER_RIGHT_36_SEQUENCE=TCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_36=636,22
PRIMER_RIGHT_36=1545,20
PRIMER_LEFT_36_TM=60.276
PRIMER_RIGHT_36_TM=58.158
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.1000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=910
PRIMER_PAIR_37_PENALTY=4.124749
PRIMER_LEFT_37_PENALTY=3.081659
PRIMER_RIGHT_37_PENALTY=1.043090
PRIMER_LEFT_37_SEQUENCE=TAGCGGCCAGTAGCTTATTT
PRIMER_RIGHT_37_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_37=633,20
PRIMER_RIGHT_37=1544,21
PRIMER_LEFT_37_TM=56.918
PRIMER_RIGHT_37_TM=60.043
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.2000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=912
PRIMER_PAIR_38_PENALTY=4.131327
PRIMER_LEFT_38_PENALTY=2.289490
PRIMER_RIGHT_38_PENALTY=1.841837
PRIMER_LEFT_38_SEQUENCE=TAGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_38_SEQUENCE=TCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_38=633,22
PRIMER_RIGHT_38=1545,20
PRIMER_LEFT_38_TM=60.289
PRIMER_RIGHT_38_TM=58.158
PRIMER_LEFT_38_GC_PERCENT=45.455
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=913
PRIMER_PAIR_39_PENALTY=4.145794
PRIMER_LEFT_39_PENALTY=3.102704
PRIMER_RIGHT_39_PENALTY=1.043090
PRIMER_LEFT_39_SEQUENCE=GATGTAAAGAGAGCGATAGCCAA
PRIMER_RIGHT_39_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_39=609,23
PRIMER_RIGHT_39=1544,21
PRIMER_LEFT_39_TM=59.897
PRIMER_RIGHT_39_TM=60.043
PRIMER_LEFT_39_GC_PERCENT=43.478
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=10.0000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=936
PRIMER_PAIR_40_PENALTY=4.175542
PRIMER_LEFT_40_PENALTY=2.740295
PRIMER_RIGHT_40_PENALTY=1.435246
PRIMER_LEFT_40_SEQUENCE=TTAGCGGCCAGTAGCTTATTT
PRIMER_RIGHT_40_SEQUENCE=GATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_40=632,21
PRIMER_RIGHT_40=1547,21
PRIMER_LEFT_40_TM=58.260
PRIMER_RIGHT_40_TM=59.565
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.2000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=916
PRIMER_PAIR_41_PENALTY=4.179349
PRIMER_LEFT_41_PENALTY=3.136259
PRIMER_RIGHT_41_PENALTY=1.043090
PRIMER_LEFT_41_SEQUENCE=TCGAAGATGTAAAGAGAGCGA
PRIMER_RIGHT_41_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_41=604,21
PRIMER_RIGHT_41=1544,21
PRIMER_LEFT_41_TM=57.864
PRIMER_RIGHT_41_TM=60.043
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=941
PRIMER_PAIR_42_PENALTY=4.187364
PRIMER_LEFT_42_PENALTY=2.752117
PRIMER_RIGHT_42_PENALTY=1.435246
PRIMER_LEFT_42_SEQUENCE=TTCGAAGATGTAAAGAGAGCGA
PRIMER_RIGHT_42_SEQUENCE=GATCCTAGTCATGCTGGTTGG
PRIMER_LEFT_42=603,22
PRIMER_RIGHT_42=1547,21
PRIMER_LEFT_42_TM=59.248
PRIMER_RIGHT_42_TM=59.565
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=945
PRIMER_PAIR_43_PENALTY=4.193385
PRIMER_LEFT_43_PENALTY=3.354433
PRIMER_RIGHT_43_PENALTY=0.838952
PRIMER_LEFT_43_SEQUENCE=TGGTAATGGAATCAGGAAGAGA
PRIMER_RIGHT_43_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_43=666,22
PRIMER_RIGHT_43=1544,20
PRIMER_LEFT_43_TM=58.646
PRIMER_RIGHT_43_TM=59.161
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=879
PRIMER_PAIR_44_PENALTY=4.220657
PRIMER_LEFT_44_PENALTY=3.177567
PRIMER_RIGHT_44_PENALTY=1.043090
PRIMER_LEFT_44_SEQUENCE=TGTAAAGAGAGCGATAGCCAA
PRIMER_RIGHT_44_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_44=611,21
PRIMER_RIGHT_44=1544,21
PRIMER_LEFT_44_TM=57.822
PRIMER_RIGHT_44_TM=60.043
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.0000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=934
PRIMER_PAIR_45_PENALTY=4.263064
PRIMER_LEFT_45_PENALTY=3.424112
PRIMER_RIGHT_45_PENALTY=0.838952
PRIMER_LEFT_45_SEQUENCE=ATTAGCGGCCAGTAGCTTATTT
PRIMER_RIGHT_45_SEQUENCE=CCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_45=631,22
PRIMER_RIGHT_45=1544,20
PRIMER_LEFT_45_TM=58.576
PRIMER_RIGHT_45_TM=59.161
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.2000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=914
PRIMER_PAIR_46_PENALTY=4.289790
PRIMER_LEFT_46_PENALTY=2.447953
PRIMER_RIGHT_46_PENALTY=1.841837
PRIMER_LEFT_46_SEQUENCE=CGGCCAGTAGCTTATTTGTCT
PRIMER_RIGHT_46_SEQUENCE=TCCTAGTCATGCTGGTTGGT
PRIMER_LEFT_46=636,21
PRIMER_RIGHT_46=1545,20
PRIMER_LEFT_46_TM=58.552
PRIMER_RIGHT_46_TM=58.158
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=910
PRIMER_PAIR_47_PENALTY=4.376244
PRIMER_LEFT_47_PENALTY=2.276218
PRIMER_RIGHT_47_PENALTY=2.100026
PRIMER_LEFT_47_SEQUENCE=CGGCCAGTAGCTTATTTGTCTC
PRIMER_RIGHT_47_SEQUENCE=TCCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_47=636,22
PRIMER_RIGHT_47=1545,21
PRIMER_LEFT_47_TM=60.276
PRIMER_RIGHT_47_TM=61.100
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.1000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=910
PRIMER_PAIR_48_PENALTY=4.389516
PRIMER_LEFT_48_PENALTY=2.289490
PRIMER_RIGHT_48_PENALTY=2.100026
PRIMER_LEFT_48_SEQUENCE=TAGCGGCCAGTAGCTTATTTGT
PRIMER_RIGHT_48_SEQUENCE=TCCTAGTCATGCTGGTTGGTG
PRIMER_LEFT_48=633,22
PRIMER_RIGHT_48=1545,21
PRIMER_LEFT_48_TM=60.289
PRIMER_RIGHT_48_TM=61.100
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=913
PRIMER_PAIR_49_PENALTY=4.397523
PRIMER_LEFT_49_PENALTY=3.354433
PRIMER_RIGHT_49_PENALTY=1.043090
PRIMER_LEFT_49_SEQUENCE=TGGTAATGGAATCAGGAAGAGA
PRIMER_RIGHT_49_SEQUENCE=CCTAGTCATGCTGGTTGGTGT
PRIMER_LEFT_49=666,22
PRIMER_RIGHT_49=1544,21
PRIMER_LEFT_49_TM=58.646
PRIMER_RIGHT_49_TM=60.043
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=879
=
SEQUENCE_ID=MAGIC.433_2_5154048_5154664_oy-0_wil-2_51188_1328_TRUE
SEQUENCE_TARGET=950,716
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATATNNNNNNNNATATNNNNATANTNATNNAAAGAACTCTAAATACTTTGTACNATCAAAANTNANATTATNAACTATTTTTTCCAACATTGAACTATATGGTAAGAAATTANATNAATNTAATTATTAATTATACNTGTGAAATTTNATNANGNAANAAGATACNCAGNTACANNANGTANAANNGAATACATNGNCACANATGCNAANTTNNNAAATGTANAACNAACTACATGANNTACATCTATTTCATGTCCACATATACCAACTATATGACGCCANGGGTCAACATCATAAACATCTTTNTNNNNNNNNNNNNNNNNCAAAATCCGGTGTTGACCATCTTTGANCCGNGTNNACCAACAAAAATATTCAAAAANTATTATNAAACATTATTTTATTNAATCATTTATAGTGTTTAAAGTTATTTCGCTGCGCATAATCANATATTGAAGAAAATACTCCATAATTCGATTAACAAATAAAGTAAATGTAAAATTATGTAACAACTTAAAACATTAATCTAAANCACATATACAATTACCAAATAAANGTCAATGAATTATAGGATGGATTAAAAGTTCATAAGGTTGAGTTAGTTAGTCAAATAAAATAAAGATTGCGNGTTCTTTTTGTCTAACAACATACAAATTAATTTNNAATCAACATAAGTTCCCACCAATGATCTCAATNAAGATGATATATTTTGTATAATCTCGTCATTCATCATATCTCNTAGACTCNGTATCATCTTCATNAACAAAGGNATCNNAAAAGGAATTATGATGGTNNTTANNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8786201_8786725_zu-0_tsu-0_158471_10250_TRUE
SEQUENCE_TARGET=950,624
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGTAAGNTGCTGGCCTGTCTTCGTCGGNNTCNTNNTCNNTTGANTACAGNGANNATNTATTTATACAGTACAGATGANATATATATTNTATAAACGAAGTCAAANGCCNATCGTATAACATCAGNNNNNNNTAANTNACATGAGNTNGTGGAGCTAGTTCACGTNAAAANNGGNGCATATATTCATGTTATCGCATATGCTTAAATCTTAATTNNTGGAAGGNGAGATATACGCTAGAATATTANAAAGAGTTTCATATATCCATGNTTTTAGGGTTGTAACTTTAAATCGTACTTGATANTTTCATATATGCAGATATTNATTAACGCGGTAANGNGCACATGGTAGTTGTGATCGAGTGATTGGTGAGACTTTGAAATATATGCTAAAACGCTTCTTCCAGAATTTGAGNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAACATCTNCTATTAGAGAGGGACACCTCGTTCNGAAATCATTCTTCTAAAATAATTGATATCGAGGAAGAGAATATGAAGACATATGAACNACTANTCGATGTTTACAATCCTCTNTTGTTAGATGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGAAAGTAATAATGAGGTTACTACTTACCATATATGGATTGGTGTGCTTGCACTTATTTGATNAAATCAATTTTGGTCCAACTCTTGCAAGTGAACNTTTTTTTTNTTTTTGACAAATAAAAGTTGGCTANCCAAAGCTTTTNCCTCTTCACCAAANAAAAAAAGTTTTTCTTTGCTCGCAGCCCCTTAAATCTGAGAACCGGTTCTGGCTCTAATCATTGGTCCAACGAAAACATGCACTTGAGNCTCTTCCTCNGCCTAGCTAAAGTGCTCATGATGTCCTTTTCATGCATGTGCTNATTTTTTTNNTTTTTTNAGGTTTAGTTGTGTGCTAGCTTAAGCTNAAAAAAAANAAACTATTTGAGTTAAAAGAAAGCTATGGTTATGCAATATTAGAATTGATGAAATGGAGTCTTGGAAATATATTTNAAAAAATTGACATGTANAATAATNAAATGTGGGAACCTAATCAAATATTGTAAGAGAGTGGTCCTATATGCTACCGCTTCCTCATGGTCAAAGCCAGGTNTATATATATAACTCCAAGCCACCGCTTCCCTAGTTTCTGCTTNAATTCAGGTTTGNTGAAACCTCGGGGCTCAGTATGCATGTCAACGTGGAACTTGGGAAAGTGAAAAGATATGAGGTAAGACATGTAACAAATTCTCGGATGTTTTCATCCTTTTGCCATTAAATTGAACAATGTCTCGTTCATGCTCAAGGAGGCTCAATTAATGAACTGAACTAACCTAGAGCCACGTGTTTTCCTTCTGCCTTTTCTGATTCAAAAAGTTGACTNAGGATAATGACATACGGGTAGTTAATTATAGTGTATCACACATNTTATAAGTTGTAATATTGTCTACTTCAAACATNNNNTAGCCACCAAGTATAAACCNGGAAGTTTCTAGGGATGTATTTAATTTGGCTCGATGGGCTTTGTGTNAAAAAAAATCCAGTCGCTATTAAGTATGAGATATCTCNACCGTTCTAGTCGAATTCGTCTATTATNNATAAGTTTGCCATATACGGCCGACNACNNNNNNTTTTGCTCATGGATATTANAGATACTTNATAAAATAAAGTTATTGATAAATAAAAAAAATCAAACTAAAATGGAGTGGAGAATTCACAAGAGAATTCAANGACAGGAAATATTTATNTTGATAAGTGGATGGAAATNAGGATAAGCATTGGTCATNGGTAAAATTTGTAAATGACGGTTTATTGGTAATTAACTACGTCATNNNNGCGGAGGGATTATTTTCAGAAAAATTAAACCTNAAAATGATATTCCNGTAGGTTTCATTTCTTG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.494293
PRIMER_LEFT_0_PENALTY=2.609965
PRIMER_RIGHT_0_PENALTY=0.884328
PRIMER_LEFT_0_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_0_SEQUENCE=AGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_0=781,21
PRIMER_RIGHT_0=1778,20
PRIMER_LEFT_0_TM=58.390
PRIMER_RIGHT_0_TM=60.884
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=11.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=998
PRIMER_PAIR_1_PENALTY=3.785869
PRIMER_LEFT_1_PENALTY=2.609965
PRIMER_RIGHT_1_PENALTY=1.175904
PRIMER_LEFT_1_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_1_SEQUENCE=TGGCTTTGACCATGAGGAAG
PRIMER_LEFT_1=781,21
PRIMER_RIGHT_1=1733,20
PRIMER_LEFT_1_TM=58.390
PRIMER_RIGHT_1_TM=61.176
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=953
PRIMER_PAIR_2_PENALTY=3.805963
PRIMER_LEFT_2_PENALTY=2.609965
PRIMER_RIGHT_2_PENALTY=1.195998
PRIMER_LEFT_2_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_2_SEQUENCE=TGACCATGAGGAAGCGGTAG
PRIMER_LEFT_2=781,21
PRIMER_RIGHT_2=1727,20
PRIMER_LEFT_2_TM=58.390
PRIMER_RIGHT_2_TM=61.196
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=947
PRIMER_PAIR_3_PENALTY=4.014419
PRIMER_LEFT_3_PENALTY=3.130091
PRIMER_RIGHT_3_PENALTY=0.884328
PRIMER_LEFT_3_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_3_SEQUENCE=AGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_3=782,20
PRIMER_RIGHT_3=1778,20
PRIMER_LEFT_3_TM=56.870
PRIMER_RIGHT_3_TM=60.884
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=997
PRIMER_PAIR_4_PENALTY=4.145553
PRIMER_LEFT_4_PENALTY=3.261224
PRIMER_RIGHT_4_PENALTY=0.884328
PRIMER_LEFT_4_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_4_SEQUENCE=AGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_4=780,22
PRIMER_RIGHT_4=1778,20
PRIMER_LEFT_4_TM=58.739
PRIMER_RIGHT_4_TM=60.884
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=999
PRIMER_PAIR_5_PENALTY=4.305995
PRIMER_LEFT_5_PENALTY=3.130091
PRIMER_RIGHT_5_PENALTY=1.175904
PRIMER_LEFT_5_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_5_SEQUENCE=TGGCTTTGACCATGAGGAAG
PRIMER_LEFT_5=782,20
PRIMER_RIGHT_5=1733,20
PRIMER_LEFT_5_TM=56.870
PRIMER_RIGHT_5_TM=61.176
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=952
PRIMER_PAIR_6_PENALTY=4.326089
PRIMER_LEFT_6_PENALTY=3.130091
PRIMER_RIGHT_6_PENALTY=1.195998
PRIMER_LEFT_6_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_6_SEQUENCE=TGACCATGAGGAAGCGGTAG
PRIMER_LEFT_6=782,20
PRIMER_RIGHT_6=1727,20
PRIMER_LEFT_6_TM=56.870
PRIMER_RIGHT_6_TM=61.196
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=946
PRIMER_PAIR_7_PENALTY=4.437129
PRIMER_LEFT_7_PENALTY=3.261224
PRIMER_RIGHT_7_PENALTY=1.175904
PRIMER_LEFT_7_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_7_SEQUENCE=TGGCTTTGACCATGAGGAAG
PRIMER_LEFT_7=780,22
PRIMER_RIGHT_7=1733,20
PRIMER_LEFT_7_TM=58.739
PRIMER_RIGHT_7_TM=61.176
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.4000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=954
PRIMER_PAIR_8_PENALTY=4.457222
PRIMER_LEFT_8_PENALTY=3.261224
PRIMER_RIGHT_8_PENALTY=1.195998
PRIMER_LEFT_8_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_8_SEQUENCE=TGACCATGAGGAAGCGGTAG
PRIMER_LEFT_8=780,22
PRIMER_RIGHT_8=1727,20
PRIMER_LEFT_8_TM=58.739
PRIMER_RIGHT_8_TM=61.196
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=948
PRIMER_PAIR_9_PENALTY=5.006972
PRIMER_LEFT_9_PENALTY=2.609965
PRIMER_RIGHT_9_PENALTY=2.397007
PRIMER_LEFT_9_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_9_SEQUENCE=ACCTGGCTTTGACCATGAGG
PRIMER_LEFT_9=781,21
PRIMER_RIGHT_9=1736,20
PRIMER_LEFT_9_TM=58.390
PRIMER_RIGHT_9_TM=62.397
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=956
PRIMER_PAIR_10_PENALTY=5.090717
PRIMER_LEFT_10_PENALTY=4.206388
PRIMER_RIGHT_10_PENALTY=0.884328
PRIMER_LEFT_10_SEQUENCE=TATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_10_SEQUENCE=AGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_10=779,23
PRIMER_RIGHT_10=1778,20
PRIMER_LEFT_10_TM=58.794
PRIMER_RIGHT_10_TM=60.884
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=11.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=1000
PRIMER_PAIR_11_PENALTY=5.382292
PRIMER_LEFT_11_PENALTY=4.206388
PRIMER_RIGHT_11_PENALTY=1.175904
PRIMER_LEFT_11_SEQUENCE=TATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_11_SEQUENCE=TGGCTTTGACCATGAGGAAG
PRIMER_LEFT_11=779,23
PRIMER_RIGHT_11=1733,20
PRIMER_LEFT_11_TM=58.794
PRIMER_RIGHT_11_TM=61.176
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=955
PRIMER_PAIR_12_PENALTY=5.402386
PRIMER_LEFT_12_PENALTY=4.206388
PRIMER_RIGHT_12_PENALTY=1.195998
PRIMER_LEFT_12_SEQUENCE=TATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_12_SEQUENCE=TGACCATGAGGAAGCGGTAG
PRIMER_LEFT_12=779,23
PRIMER_RIGHT_12=1727,20
PRIMER_LEFT_12_TM=58.794
PRIMER_RIGHT_12_TM=61.196
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=949
PRIMER_PAIR_13_PENALTY=5.483399
PRIMER_LEFT_13_PENALTY=4.307495
PRIMER_RIGHT_13_PENALTY=1.175904
PRIMER_LEFT_13_SEQUENCE=CTATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_13_SEQUENCE=TGGCTTTGACCATGAGGAAG
PRIMER_LEFT_13=778,24
PRIMER_RIGHT_13=1733,20
PRIMER_LEFT_13_TM=59.693
PRIMER_RIGHT_13_TM=61.176
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=956
PRIMER_PAIR_14_PENALTY=5.503493
PRIMER_LEFT_14_PENALTY=4.307495
PRIMER_RIGHT_14_PENALTY=1.195998
PRIMER_LEFT_14_SEQUENCE=CTATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_14_SEQUENCE=TGACCATGAGGAAGCGGTAG
PRIMER_LEFT_14=778,24
PRIMER_RIGHT_14=1727,20
PRIMER_LEFT_14_TM=59.693
PRIMER_RIGHT_14_TM=61.196
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=950
PRIMER_PAIR_15_PENALTY=5.527098
PRIMER_LEFT_15_PENALTY=3.130091
PRIMER_RIGHT_15_PENALTY=2.397007
PRIMER_LEFT_15_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_15_SEQUENCE=ACCTGGCTTTGACCATGAGG
PRIMER_LEFT_15=782,20
PRIMER_RIGHT_15=1736,20
PRIMER_LEFT_15_TM=56.870
PRIMER_RIGHT_15_TM=62.397
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=955
PRIMER_PAIR_16_PENALTY=5.613026
PRIMER_LEFT_16_PENALTY=2.609965
PRIMER_RIGHT_16_PENALTY=3.003061
PRIMER_LEFT_16_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_16_SEQUENCE=CTTTGACCATGAGGAAGCGG
PRIMER_LEFT_16=781,21
PRIMER_RIGHT_16=1730,20
PRIMER_LEFT_16_TM=58.390
PRIMER_RIGHT_16_TM=63.003
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=11.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=950
PRIMER_PAIR_17_PENALTY=5.646669
PRIMER_LEFT_17_PENALTY=2.609965
PRIMER_RIGHT_17_PENALTY=3.036704
PRIMER_LEFT_17_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_17_SEQUENCE=ACCATGAGGAAGCGGTAGCA
PRIMER_LEFT_17=781,21
PRIMER_RIGHT_17=1725,20
PRIMER_LEFT_17_TM=58.390
PRIMER_RIGHT_17_TM=63.037
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=945
PRIMER_PAIR_18_PENALTY=5.658231
PRIMER_LEFT_18_PENALTY=3.261224
PRIMER_RIGHT_18_PENALTY=2.397007
PRIMER_LEFT_18_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_18_SEQUENCE=ACCTGGCTTTGACCATGAGG
PRIMER_LEFT_18=780,22
PRIMER_RIGHT_18=1736,20
PRIMER_LEFT_18_TM=58.739
PRIMER_RIGHT_18_TM=62.397
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=957
PRIMER_PAIR_19_PENALTY=5.666030
PRIMER_LEFT_19_PENALTY=2.609965
PRIMER_RIGHT_19_PENALTY=3.056065
PRIMER_LEFT_19_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_19_SEQUENCE=AAGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_19=781,21
PRIMER_RIGHT_19=1779,21
PRIMER_LEFT_19_TM=58.390
PRIMER_RIGHT_19_TM=62.056
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=11.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=999
PRIMER_PAIR_20_PENALTY=5.683694
PRIMER_LEFT_20_PENALTY=2.609965
PRIMER_RIGHT_20_PENALTY=3.073730
PRIMER_LEFT_20_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_20_SEQUENCE=CTGGCTTTGACCATGAGGAAG
PRIMER_LEFT_20=781,21
PRIMER_RIGHT_20=1734,21
PRIMER_LEFT_20_TM=58.390
PRIMER_RIGHT_20_TM=62.074
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=954
PRIMER_PAIR_21_PENALTY=5.925022
PRIMER_LEFT_21_PENALTY=5.793882
PRIMER_RIGHT_21_PENALTY=0.131140
PRIMER_LEFT_21_SEQUENCE=CGAGGAAGAGAATATGAAGACA
PRIMER_RIGHT_21_SEQUENCE=CAGAAACTAGGGAAGCGGTG
PRIMER_LEFT_21=831,22
PRIMER_RIGHT_21=1776,20
PRIMER_LEFT_21_TM=56.206
PRIMER_RIGHT_21_TM=59.869
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=9.9000
PRIMER_PAIR_21_COMPL_ANY=2.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=946
PRIMER_PAIR_22_PENALTY=6.023558
PRIMER_LEFT_22_PENALTY=1.104169
PRIMER_RIGHT_22_PENALTY=4.919389
PRIMER_LEFT_22_SEQUENCE=TTGTGATCGAGTGATTGGTGA
PRIMER_RIGHT_22_SEQUENCE=TTCCAAGACTCCATTTCATC
PRIMER_LEFT_22=651,21
PRIMER_RIGHT_22=1628,20
PRIMER_LEFT_22_TM=60.104
PRIMER_RIGHT_22_TM=55.081
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=978
PRIMER_PAIR_23_PENALTY=6.054938
PRIMER_LEFT_23_PENALTY=2.609965
PRIMER_RIGHT_23_PENALTY=3.444973
PRIMER_LEFT_23_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_23_SEQUENCE=TTGACCATGAGGAAGCGGTAG
PRIMER_LEFT_23=781,21
PRIMER_RIGHT_23=1728,21
PRIMER_LEFT_23_TM=58.390
PRIMER_RIGHT_23_TM=62.445
PRIMER_LEFT_23_GC_PERCENT=52.381
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=948
PRIMER_PAIR_24_PENALTY=6.085764
PRIMER_LEFT_24_PENALTY=2.609965
PRIMER_RIGHT_24_PENALTY=3.475800
PRIMER_LEFT_24_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_24_SEQUENCE=CCTGGCTTTGACCATGAGGA
PRIMER_LEFT_24=781,21
PRIMER_RIGHT_24=1735,20
PRIMER_LEFT_24_TM=58.390
PRIMER_RIGHT_24_TM=63.476
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=955
PRIMER_PAIR_25_PENALTY=6.133152
PRIMER_LEFT_25_PENALTY=3.130091
PRIMER_RIGHT_25_PENALTY=3.003061
PRIMER_LEFT_25_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_25_SEQUENCE=CTTTGACCATGAGGAAGCGG
PRIMER_LEFT_25=782,20
PRIMER_RIGHT_25=1730,20
PRIMER_LEFT_25_TM=56.870
PRIMER_RIGHT_25_TM=63.003
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=11.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=949
PRIMER_PAIR_26_PENALTY=6.166795
PRIMER_LEFT_26_PENALTY=3.130091
PRIMER_RIGHT_26_PENALTY=3.036704
PRIMER_LEFT_26_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_26_SEQUENCE=ACCATGAGGAAGCGGTAGCA
PRIMER_LEFT_26=782,20
PRIMER_RIGHT_26=1725,20
PRIMER_LEFT_26_TM=56.870
PRIMER_RIGHT_26_TM=63.037
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=7.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=944
PRIMER_PAIR_27_PENALTY=6.186156
PRIMER_LEFT_27_PENALTY=3.130091
PRIMER_RIGHT_27_PENALTY=3.056065
PRIMER_LEFT_27_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_27_SEQUENCE=AAGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_27=782,20
PRIMER_RIGHT_27=1779,21
PRIMER_LEFT_27_TM=56.870
PRIMER_RIGHT_27_TM=62.056
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=11.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=998
PRIMER_PAIR_28_PENALTY=6.203821
PRIMER_LEFT_28_PENALTY=3.130091
PRIMER_RIGHT_28_PENALTY=3.073730
PRIMER_LEFT_28_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_28_SEQUENCE=CTGGCTTTGACCATGAGGAAG
PRIMER_LEFT_28=782,20
PRIMER_RIGHT_28=1734,21
PRIMER_LEFT_28_TM=56.870
PRIMER_RIGHT_28_TM=62.074
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=953
PRIMER_PAIR_29_PENALTY=6.227794
PRIMER_LEFT_29_PENALTY=1.308405
PRIMER_RIGHT_29_PENALTY=4.919389
PRIMER_LEFT_29_SEQUENCE=TGTGATCGAGTGATTGGTGAG
PRIMER_RIGHT_29_SEQUENCE=TTCCAAGACTCCATTTCATC
PRIMER_LEFT_29=652,21
PRIMER_RIGHT_29=1628,20
PRIMER_LEFT_29_TM=59.692
PRIMER_RIGHT_29_TM=55.081
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=977
PRIMER_PAIR_30_PENALTY=6.264285
PRIMER_LEFT_30_PENALTY=3.261224
PRIMER_RIGHT_30_PENALTY=3.003061
PRIMER_LEFT_30_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_30_SEQUENCE=CTTTGACCATGAGGAAGCGG
PRIMER_LEFT_30=780,22
PRIMER_RIGHT_30=1730,20
PRIMER_LEFT_30_TM=58.739
PRIMER_RIGHT_30_TM=63.003
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.4000
PRIMER_RIGHT_30_END_STABILITY=11.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=951
PRIMER_PAIR_31_PENALTY=6.297928
PRIMER_LEFT_31_PENALTY=3.261224
PRIMER_RIGHT_31_PENALTY=3.036704
PRIMER_LEFT_31_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_31_SEQUENCE=ACCATGAGGAAGCGGTAGCA
PRIMER_LEFT_31=780,22
PRIMER_RIGHT_31=1725,20
PRIMER_LEFT_31_TM=58.739
PRIMER_RIGHT_31_TM=63.037
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=7.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=946
PRIMER_PAIR_32_PENALTY=6.317290
PRIMER_LEFT_32_PENALTY=3.261224
PRIMER_RIGHT_32_PENALTY=3.056065
PRIMER_LEFT_32_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_32_SEQUENCE=AAGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_32=780,22
PRIMER_RIGHT_32=1779,21
PRIMER_LEFT_32_TM=58.739
PRIMER_RIGHT_32_TM=62.056
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=11.4000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=1000
PRIMER_PAIR_33_PENALTY=6.334954
PRIMER_LEFT_33_PENALTY=3.261224
PRIMER_RIGHT_33_PENALTY=3.073730
PRIMER_LEFT_33_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_33_SEQUENCE=CTGGCTTTGACCATGAGGAAG
PRIMER_LEFT_33=780,22
PRIMER_RIGHT_33=1734,21
PRIMER_LEFT_33_TM=58.739
PRIMER_RIGHT_33_TM=62.074
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=955
PRIMER_PAIR_34_PENALTY=6.414761
PRIMER_LEFT_34_PENALTY=5.793882
PRIMER_RIGHT_34_PENALTY=0.620879
PRIMER_LEFT_34_SEQUENCE=CGAGGAAGAGAATATGAAGACA
PRIMER_RIGHT_34_SEQUENCE=AGAAACTAGGGAAGCGGTGG
PRIMER_LEFT_34=831,22
PRIMER_RIGHT_34=1775,20
PRIMER_LEFT_34_TM=56.206
PRIMER_RIGHT_34_TM=60.621
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=2.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=945
PRIMER_PAIR_35_PENALTY=6.419643
PRIMER_LEFT_35_PENALTY=2.609965
PRIMER_RIGHT_35_PENALTY=3.809679
PRIMER_LEFT_35_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_35_SEQUENCE=CGGTAGCATATAGGACCACTCTC
PRIMER_LEFT_35=781,21
PRIMER_RIGHT_35=1713,23
PRIMER_LEFT_35_TM=58.390
PRIMER_RIGHT_35_TM=59.190
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=52.174
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.4000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=933
PRIMER_PAIR_36_PENALTY=6.475237
PRIMER_LEFT_36_PENALTY=2.609965
PRIMER_RIGHT_36_PENALTY=3.865272
PRIMER_LEFT_36_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_36_SEQUENCE=GCGGTAGCATATAGGACCACTCT
PRIMER_LEFT_36=781,21
PRIMER_RIGHT_36=1714,23
PRIMER_LEFT_36_TM=58.390
PRIMER_RIGHT_36_TM=60.865
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=52.174
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.4000
PRIMER_RIGHT_36_END_STABILITY=6.1000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=934
PRIMER_PAIR_37_PENALTY=6.575064
PRIMER_LEFT_37_PENALTY=3.130091
PRIMER_RIGHT_37_PENALTY=3.444973
PRIMER_LEFT_37_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_37_SEQUENCE=TTGACCATGAGGAAGCGGTAG
PRIMER_LEFT_37=782,20
PRIMER_RIGHT_37=1728,21
PRIMER_LEFT_37_TM=56.870
PRIMER_RIGHT_37_TM=62.445
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=947
PRIMER_PAIR_38_PENALTY=6.599858
PRIMER_LEFT_38_PENALTY=1.680469
PRIMER_RIGHT_38_PENALTY=4.919389
PRIMER_LEFT_38_SEQUENCE=TGCTAAAACGCTTCTTCCAG
PRIMER_RIGHT_38_SEQUENCE=TTCCAAGACTCCATTTCATC
PRIMER_LEFT_38=686,20
PRIMER_RIGHT_38=1628,20
PRIMER_LEFT_38_TM=58.320
PRIMER_RIGHT_38_TM=55.081
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=943
PRIMER_PAIR_39_PENALTY=6.603395
PRIMER_LEFT_39_PENALTY=4.206388
PRIMER_RIGHT_39_PENALTY=2.397007
PRIMER_LEFT_39_SEQUENCE=TATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_39_SEQUENCE=ACCTGGCTTTGACCATGAGG
PRIMER_LEFT_39=779,23
PRIMER_RIGHT_39=1736,20
PRIMER_LEFT_39_TM=58.794
PRIMER_RIGHT_39_TM=62.397
PRIMER_LEFT_39_GC_PERCENT=47.826
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=958
PRIMER_PAIR_40_PENALTY=6.605891
PRIMER_LEFT_40_PENALTY=3.130091
PRIMER_RIGHT_40_PENALTY=3.475800
PRIMER_LEFT_40_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_40_SEQUENCE=CCTGGCTTTGACCATGAGGA
PRIMER_LEFT_40=782,20
PRIMER_RIGHT_40=1735,20
PRIMER_LEFT_40_TM=56.870
PRIMER_RIGHT_40_TM=63.476
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.4000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=954
PRIMER_PAIR_41_PENALTY=6.673071
PRIMER_LEFT_41_PENALTY=2.609965
PRIMER_RIGHT_41_PENALTY=4.063106
PRIMER_LEFT_41_SEQUENCE=TTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_41_SEQUENCE=CGGTAGCATATAGGACCACTCTCT
PRIMER_LEFT_41=781,21
PRIMER_RIGHT_41=1713,24
PRIMER_LEFT_41_TM=58.390
PRIMER_RIGHT_41_TM=60.063
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.4000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=933
PRIMER_PAIR_42_PENALTY=6.678211
PRIMER_LEFT_42_PENALTY=5.793882
PRIMER_RIGHT_42_PENALTY=0.884328
PRIMER_LEFT_42_SEQUENCE=CGAGGAAGAGAATATGAAGACA
PRIMER_RIGHT_42_SEQUENCE=AGCAGAAACTAGGGAAGCGG
PRIMER_LEFT_42=831,22
PRIMER_RIGHT_42=1778,20
PRIMER_LEFT_42_TM=56.206
PRIMER_RIGHT_42_TM=60.884
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=11.4000
PRIMER_PAIR_42_COMPL_ANY=2.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=948
PRIMER_PAIR_43_PENALTY=6.704502
PRIMER_LEFT_43_PENALTY=4.307495
PRIMER_RIGHT_43_PENALTY=2.397007
PRIMER_LEFT_43_SEQUENCE=CTATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_43_SEQUENCE=ACCTGGCTTTGACCATGAGG
PRIMER_LEFT_43=778,24
PRIMER_RIGHT_43=1736,20
PRIMER_LEFT_43_TM=59.693
PRIMER_RIGHT_43_TM=62.397
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=959
PRIMER_PAIR_44_PENALTY=6.706197
PRIMER_LEFT_44_PENALTY=3.261224
PRIMER_RIGHT_44_PENALTY=3.444973
PRIMER_LEFT_44_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_44_SEQUENCE=TTGACCATGAGGAAGCGGTAG
PRIMER_LEFT_44=780,22
PRIMER_RIGHT_44=1728,21
PRIMER_LEFT_44_TM=58.739
PRIMER_RIGHT_44_TM=62.445
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.4000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=949
PRIMER_PAIR_45_PENALTY=6.737024
PRIMER_LEFT_45_PENALTY=3.261224
PRIMER_RIGHT_45_PENALTY=3.475800
PRIMER_LEFT_45_SEQUENCE=ATTAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_45_SEQUENCE=CCTGGCTTTGACCATGAGGA
PRIMER_LEFT_45=780,22
PRIMER_RIGHT_45=1735,20
PRIMER_LEFT_45_TM=58.739
PRIMER_RIGHT_45_TM=63.476
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.4000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=956
PRIMER_PAIR_46_PENALTY=6.845673
PRIMER_LEFT_46_PENALTY=6.714533
PRIMER_RIGHT_46_PENALTY=0.131140
PRIMER_LEFT_46_SEQUENCE=TCGAGGAAGAGAATATGAAGAC
PRIMER_RIGHT_46_SEQUENCE=CAGAAACTAGGGAAGCGGTG
PRIMER_LEFT_46=830,22
PRIMER_RIGHT_46=1776,20
PRIMER_LEFT_46_TM=55.285
PRIMER_RIGHT_46_TM=59.869
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=947
PRIMER_PAIR_47_PENALTY=6.899655
PRIMER_LEFT_47_PENALTY=1.980266
PRIMER_RIGHT_47_PENALTY=4.919389
PRIMER_LEFT_47_SEQUENCE=TTGTGATCGAGTGATTGGTG
PRIMER_RIGHT_47_SEQUENCE=TTCCAAGACTCCATTTCATC
PRIMER_LEFT_47=651,20
PRIMER_RIGHT_47=1628,20
PRIMER_LEFT_47_TM=58.020
PRIMER_RIGHT_47_TM=55.081
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.6000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=978
PRIMER_PAIR_48_PENALTY=6.939770
PRIMER_LEFT_48_PENALTY=3.130091
PRIMER_RIGHT_48_PENALTY=3.809679
PRIMER_LEFT_48_SEQUENCE=TAGAGAGGGACACCTCGTTC
PRIMER_RIGHT_48_SEQUENCE=CGGTAGCATATAGGACCACTCTC
PRIMER_LEFT_48=782,20
PRIMER_RIGHT_48=1713,23
PRIMER_LEFT_48_TM=56.870
PRIMER_RIGHT_48_TM=59.190
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=52.174
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=932
PRIMER_PAIR_49_PENALTY=6.969786
PRIMER_LEFT_49_PENALTY=5.793882
PRIMER_RIGHT_49_PENALTY=1.175904
PRIMER_LEFT_49_SEQUENCE=CGAGGAAGAGAATATGAAGACA
PRIMER_RIGHT_49_SEQUENCE=TGGCTTTGACCATGAGGAAG
PRIMER_LEFT_49=831,22
PRIMER_RIGHT_49=1733,20
PRIMER_LEFT_49_TM=56.206
PRIMER_RIGHT_49_TM=61.176
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=903
=
SEQUENCE_ID=MAGIC.149_4_8621550_8621676_wil-2_edi-0_330308_251509_TRUE
SEQUENCE_TARGET=950,226
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNGNGATCAAAGNTACTTGCACGNTGAAGANTCGTCGGNGCATATCGTGGGAAGAGATAAACGAGAGNNGGTTGAANGGCTTATGTNTGTTTTGCNAAGANCNAGAANCTCCNGATCACCACCTGAAACACANNAACTTAGGGNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTGAACCTAGGNAAGTANATTTCGGNTTGGAAAATNTNGATTCTGTNGNGNTGAACGAGNATCAGGAGCANGATTGGGTTAAACAGAACATNNNTAAGAGNTTTGAGGTTGANGATGATGTTNATANGGGTCANAAATTGTTGAGTATTACAGAAAATTGNAGTGCACACCAAGTNTTNGGTAAAACGTCTCAACATGAAGAAAANCTGGAAATAAAGAAGAAAGNGAAAGGATTCAAATCATGGATGTTCAAATTCAAACTTGCTANGAAAANAATAAGGAAACGAGACAANNNNNGNTGGTTCCACANTTGGNGCNNAAAANTGGGTTGGAGGAAGANCAAGCCTATGAAATTGCNGTNAGTTTCACATCANGTGAAAGANTCATNACATAGGGATAANGACTCTTNTGCGGNAAAAAAGTCAATTAAGCAAGNGATGNGTGTTGAAGTGGAGAGCTTGTNGCAGCAACAGGATTTAANACGCATNAAAGAGACACAAGCGGATNAGGGAGATTCTTATGTTGTTGTNCNGAAACTGTTGCCGCATCTTGAANCGGTTTGTGCNACAAAATCGTTTTTGGANNNTGATTCAATNTCNGAACTTGAAGNGGAGTTAACTGACTCAANGATGAGGACGGCTNGTGTTACACAACATCANGTNTCAANTTTTNTCATGGAAACTAGCCATCATCATCAAGTGTTAGAGGATTTTGATATGCATATGGTCATTGAGGATCAACAACCTATCGTGACAGAAGCTTTGCGTNTAATCTCTAANGATTTCCACTCGGACGTGGCNGAGTTGTGTGAGCAGTTAACATGTGACGAGTCTCATAAACCGAAGCAAAGACTANCTCCAAAGTCTTGGATGTTTAAGTATAGAAGGGACCATAAGATACTACANAGCNCTACTATGTCTAGGAATGTGAGAATGGTNGNTTTCAGAAAAATGGAAGCAGCNGANAGGANTCAANATTTGGTTACACANAGGTTTCATGNGGGTCCACTANAAATGGATANTCAAGACTCTGAANTTGGTATCANAGNNGTTTTANGAAGAGTGATGATGNTGTTCCAANACANTATTGTACAAGCCAAGATGATNGTGACAAAGAGAAAGAAAACGTTTTACAAGTCTAGGAGATTCAAGTTCAAACATAGAGTTGTACCAAGAGCTTTACAATATGGTTTTGAAGTCAAAATGAAGCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCAAGGAGGCAGAACTTGTTCATANTTTGCTTNNGNATNNNNNNNNNNNGTTNAACTTGAAGAAAAAGGGGAGATTTAGACTCTTTCAGTCATTTTCTAGACAACTTCTCTTATGGCTGNNNCATGATNCATTGCTAATNAAANCGTTTCTGCTTAGTGATGAGAGGTTCCAAATAAAGCATANATGGAGAACTAAGTTCATACACTCNGTTTCANCAATTGNTNNNGNNCNTTATGAATATTCTATTTGGANACAGAGTGACANAGGGACGCAGCTNTTNTACAGCAGGAGATTGGTTGTTTNCAAGCTTGGAAGTGAGGCCAAGAATGTTGTAGAGGTGTCTCANCGTGTGNNNNNNNNNNNNNNNNTGNAATTTGCCAAANACGAACGAAAGCTTGAAGTNNNACAGAGCCAAACCTTNNNNNNNNNNNNNNNNNNNNNNNCACAAGAGCTACTTGAGAATACCTNTTCGNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.164686
PRIMER_LEFT_0_PENALTY=1.748344
PRIMER_RIGHT_0_PENALTY=1.416342
PRIMER_LEFT_0_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_0_SEQUENCE=TGAACAAGTTCTGCCTCCTTG
PRIMER_LEFT_0=891,20
PRIMER_RIGHT_0=1628,21
PRIMER_LEFT_0_TM=61.748
PRIMER_RIGHT_0_TM=60.416
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=738
PRIMER_PAIR_1_PENALTY=3.872999
PRIMER_LEFT_1_PENALTY=1.748344
PRIMER_RIGHT_1_PENALTY=2.124655
PRIMER_LEFT_1_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_1_SEQUENCE=TGGAACCTCTCATCACTAAGCA
PRIMER_LEFT_1=891,20
PRIMER_RIGHT_1=1778,22
PRIMER_LEFT_1_TM=61.748
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=888
PRIMER_PAIR_2_PENALTY=4.233911
PRIMER_LEFT_2_PENALTY=1.748344
PRIMER_RIGHT_2_PENALTY=2.485568
PRIMER_LEFT_2_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_2_SEQUENCE=GAACAAGTTCTGCCTCCTTG
PRIMER_LEFT_2=891,20
PRIMER_RIGHT_2=1627,20
PRIMER_LEFT_2_TM=61.748
PRIMER_RIGHT_2_TM=57.514
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=737
PRIMER_PAIR_3_PENALTY=4.233911
PRIMER_LEFT_3_PENALTY=1.748344
PRIMER_RIGHT_3_PENALTY=2.485568
PRIMER_LEFT_3_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_3_SEQUENCE=TGAACAAGTTCTGCCTCCTT
PRIMER_LEFT_3=891,20
PRIMER_RIGHT_3=1628,20
PRIMER_LEFT_3_TM=61.748
PRIMER_RIGHT_3_TM=57.514
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=738
PRIMER_PAIR_4_PENALTY=4.409057
PRIMER_LEFT_4_PENALTY=1.748344
PRIMER_RIGHT_4_PENALTY=2.660713
PRIMER_LEFT_4_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_4_SEQUENCE=ATGAACAAGTTCTGCCTCCTTG
PRIMER_LEFT_4=891,20
PRIMER_RIGHT_4=1629,22
PRIMER_LEFT_4_TM=61.748
PRIMER_RIGHT_4_TM=60.661
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=739
PRIMER_PAIR_5_PENALTY=4.826146
PRIMER_LEFT_5_PENALTY=1.748344
PRIMER_RIGHT_5_PENALTY=3.077802
PRIMER_LEFT_5_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_5_SEQUENCE=ATGAACAAGTTCTGCCTCCTT
PRIMER_LEFT_5=891,20
PRIMER_RIGHT_5=1629,21
PRIMER_LEFT_5_TM=61.748
PRIMER_RIGHT_5_TM=57.922
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=739
PRIMER_PAIR_6_PENALTY=5.341153
PRIMER_LEFT_6_PENALTY=1.748344
PRIMER_RIGHT_6_PENALTY=3.592809
PRIMER_LEFT_6_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_6_SEQUENCE=ATGAACAAGTTCTGCCTCCT
PRIMER_LEFT_6=891,20
PRIMER_RIGHT_6=1629,20
PRIMER_LEFT_6_TM=61.748
PRIMER_RIGHT_6_TM=56.407
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=739
PRIMER_PAIR_7_PENALTY=5.395559
PRIMER_LEFT_7_PENALTY=1.748344
PRIMER_RIGHT_7_PENALTY=3.647215
PRIMER_LEFT_7_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_7_SEQUENCE=TATGAACAAGTTCTGCCTCCTTG
PRIMER_LEFT_7=891,20
PRIMER_RIGHT_7=1630,23
PRIMER_LEFT_7_TM=61.748
PRIMER_RIGHT_7_TM=60.647
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=740
PRIMER_PAIR_8_PENALTY=5.545392
PRIMER_LEFT_8_PENALTY=1.748344
PRIMER_RIGHT_8_PENALTY=3.797048
PRIMER_LEFT_8_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_8_SEQUENCE=TGGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_8=891,20
PRIMER_RIGHT_8=1778,23
PRIMER_LEFT_8_TM=61.748
PRIMER_RIGHT_8_TM=60.797
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=888
PRIMER_PAIR_9_PENALTY=5.666269
PRIMER_LEFT_9_PENALTY=1.748344
PRIMER_RIGHT_9_PENALTY=3.917925
PRIMER_LEFT_9_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_9_SEQUENCE=GGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_9=891,20
PRIMER_RIGHT_9=1777,22
PRIMER_LEFT_9_TM=61.748
PRIMER_RIGHT_9_TM=58.082
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=887
PRIMER_PAIR_10_PENALTY=5.739555
PRIMER_LEFT_10_PENALTY=1.748344
PRIMER_RIGHT_10_PENALTY=3.991211
PRIMER_LEFT_10_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_10_SEQUENCE=TATGAACAAGTTCTGCCTCCTT
PRIMER_LEFT_10=891,20
PRIMER_RIGHT_10=1630,22
PRIMER_LEFT_10_TM=61.748
PRIMER_RIGHT_10_TM=58.009
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=740
PRIMER_PAIR_11_PENALTY=5.748386
PRIMER_LEFT_11_PENALTY=1.748344
PRIMER_RIGHT_11_PENALTY=4.000042
PRIMER_LEFT_11_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_11_SEQUENCE=GGAACCTCTCATCACTAAGCA
PRIMER_LEFT_11=891,20
PRIMER_RIGHT_11=1777,21
PRIMER_LEFT_11_TM=61.748
PRIMER_RIGHT_11_TM=57.000
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=887
PRIMER_PAIR_12_PENALTY=5.895799
PRIMER_LEFT_12_PENALTY=1.748344
PRIMER_RIGHT_12_PENALTY=4.147455
PRIMER_LEFT_12_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_12_SEQUENCE=TTGGAACCTCTCATCACTAAGCA
PRIMER_LEFT_12=891,20
PRIMER_RIGHT_12=1779,23
PRIMER_LEFT_12_TM=61.748
PRIMER_RIGHT_12_TM=61.147
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=889
PRIMER_PAIR_13_PENALTY=6.107439
PRIMER_LEFT_13_PENALTY=1.748344
PRIMER_RIGHT_13_PENALTY=4.359095
PRIMER_LEFT_13_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_13_SEQUENCE=GCTTTATTTGGAACCTCTCATCAC
PRIMER_LEFT_13=891,20
PRIMER_RIGHT_13=1786,24
PRIMER_LEFT_13_TM=61.748
PRIMER_RIGHT_13_TM=60.359
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=41.667
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=896
PRIMER_PAIR_14_PENALTY=6.109287
PRIMER_LEFT_14_PENALTY=3.984632
PRIMER_RIGHT_14_PENALTY=2.124655
PRIMER_LEFT_14_SEQUENCE=GTGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_14_SEQUENCE=TGGAACCTCTCATCACTAAGCA
PRIMER_LEFT_14=800,21
PRIMER_RIGHT_14=1778,22
PRIMER_LEFT_14_TM=57.015
PRIMER_RIGHT_14_TM=59.875
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=979
PRIMER_PAIR_15_PENALTY=6.146965
PRIMER_LEFT_15_PENALTY=4.022310
PRIMER_RIGHT_15_PENALTY=2.124655
PRIMER_LEFT_15_SEQUENCE=TGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_15_SEQUENCE=TGGAACCTCTCATCACTAAGCA
PRIMER_LEFT_15=801,20
PRIMER_RIGHT_15=1778,22
PRIMER_LEFT_15_TM=55.978
PRIMER_RIGHT_15_TM=59.875
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.455
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=978
PRIMER_PAIR_16_PENALTY=6.146965
PRIMER_LEFT_16_PENALTY=4.022310
PRIMER_RIGHT_16_PENALTY=2.124655
PRIMER_LEFT_16_SEQUENCE=GTGTTGAAGTGGAGAGCTTG
PRIMER_RIGHT_16_SEQUENCE=TGGAACCTCTCATCACTAAGCA
PRIMER_LEFT_16=800,20
PRIMER_RIGHT_16=1778,22
PRIMER_LEFT_16_TM=55.978
PRIMER_RIGHT_16_TM=59.875
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=979
PRIMER_PAIR_17_PENALTY=6.191167
PRIMER_LEFT_17_PENALTY=1.748344
PRIMER_RIGHT_17_PENALTY=4.442823
PRIMER_LEFT_17_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_17_SEQUENCE=TATGAACAAGTTCTGCCTCCT
PRIMER_LEFT_17=891,20
PRIMER_RIGHT_17=1630,21
PRIMER_LEFT_17_TM=61.748
PRIMER_RIGHT_17_TM=56.557
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=740
PRIMER_PAIR_18_PENALTY=6.376356
PRIMER_LEFT_18_PENALTY=1.748344
PRIMER_RIGHT_18_PENALTY=4.628012
PRIMER_LEFT_18_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_18_SEQUENCE=TATGAACAAGTTCTGCCTCC
PRIMER_LEFT_18=891,20
PRIMER_RIGHT_18=1630,20
PRIMER_LEFT_18_TM=61.748
PRIMER_RIGHT_18_TM=55.372
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=740
PRIMER_PAIR_19_PENALTY=6.657131
PRIMER_LEFT_19_PENALTY=1.748344
PRIMER_RIGHT_19_PENALTY=4.908787
PRIMER_LEFT_19_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_19_SEQUENCE=GAACCTCTCATCACTAAGCAGAAA
PRIMER_LEFT_19=891,20
PRIMER_RIGHT_19=1776,24
PRIMER_LEFT_19_TM=61.748
PRIMER_RIGHT_19_TM=59.091
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=41.667
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=886
PRIMER_PAIR_20_PENALTY=6.734466
PRIMER_LEFT_20_PENALTY=1.748344
PRIMER_RIGHT_20_PENALTY=4.986122
PRIMER_LEFT_20_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_20_SEQUENCE=GCTTTATTTGGAACCTCTCATC
PRIMER_LEFT_20=891,20
PRIMER_RIGHT_20=1786,22
PRIMER_LEFT_20_TM=61.748
PRIMER_RIGHT_20_TM=57.014
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=896
PRIMER_PAIR_21_PENALTY=6.862476
PRIMER_LEFT_21_PENALTY=1.748344
PRIMER_RIGHT_21_PENALTY=5.114132
PRIMER_LEFT_21_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_21_SEQUENCE=GGAACCTCTCATCACTAAGCAGAA
PRIMER_LEFT_21=891,20
PRIMER_RIGHT_21=1777,24
PRIMER_LEFT_21_TM=61.748
PRIMER_RIGHT_21_TM=61.114
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.833
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=887
PRIMER_PAIR_22_PENALTY=6.908118
PRIMER_LEFT_22_PENALTY=1.748344
PRIMER_RIGHT_22_PENALTY=5.159774
PRIMER_LEFT_22_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_22_SEQUENCE=GACTGAAAGAGTCTAAATCTCCCC
PRIMER_LEFT_22=891,20
PRIMER_RIGHT_22=1697,24
PRIMER_LEFT_22_TM=61.748
PRIMER_RIGHT_22_TM=58.840
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.833
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=10.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=807
PRIMER_PAIR_23_PENALTY=6.984791
PRIMER_LEFT_23_PENALTY=1.748344
PRIMER_RIGHT_23_PENALTY=5.236447
PRIMER_LEFT_23_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_23_SEQUENCE=GAACCTCTCATCACTAAGCAGAA
PRIMER_LEFT_23=891,20
PRIMER_RIGHT_23=1776,23
PRIMER_LEFT_23_TM=61.748
PRIMER_RIGHT_23_TM=57.764
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=886
PRIMER_PAIR_24_PENALTY=7.046405
PRIMER_LEFT_24_PENALTY=1.748344
PRIMER_RIGHT_24_PENALTY=5.298061
PRIMER_LEFT_24_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_24_SEQUENCE=GACTGAAAGAGTCTAAATCTCCCCT
PRIMER_LEFT_24=891,20
PRIMER_RIGHT_24=1697,25
PRIMER_LEFT_24_TM=61.748
PRIMER_RIGHT_24_TM=59.702
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=44.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=10.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=807
PRIMER_PAIR_25_PENALTY=7.202638
PRIMER_LEFT_25_PENALTY=1.748344
PRIMER_RIGHT_25_PENALTY=5.454294
PRIMER_LEFT_25_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_25_SEQUENCE=CTGAAAGAGTCTAAATCTCCCCTT
PRIMER_LEFT_25=891,20
PRIMER_RIGHT_25=1695,24
PRIMER_LEFT_25_TM=61.748
PRIMER_RIGHT_25_TM=58.546
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=41.667
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=805
PRIMER_PAIR_26_PENALTY=7.466830
PRIMER_LEFT_26_PENALTY=1.748344
PRIMER_RIGHT_26_PENALTY=5.718486
PRIMER_LEFT_26_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_26_SEQUENCE=ACTGAAAGAGTCTAAATCTCCCCTT
PRIMER_LEFT_26=891,20
PRIMER_RIGHT_26=1696,25
PRIMER_LEFT_26_TM=61.748
PRIMER_RIGHT_26_TM=59.282
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=806
PRIMER_PAIR_27_PENALTY=7.468560
PRIMER_LEFT_27_PENALTY=3.984632
PRIMER_RIGHT_27_PENALTY=3.483928
PRIMER_LEFT_27_SEQUENCE=GTGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_27_SEQUENCE=GTCTTTGCTTCGGTTTATGAGA
PRIMER_LEFT_27=800,21
PRIMER_RIGHT_27=1210,22
PRIMER_LEFT_27_TM=57.015
PRIMER_RIGHT_27_TM=58.516
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=411
PRIMER_PAIR_28_PENALTY=7.480206
PRIMER_LEFT_28_PENALTY=1.748344
PRIMER_RIGHT_28_PENALTY=5.731862
PRIMER_LEFT_28_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_28_SEQUENCE=CTGAAAGAGTCTAAATCTCCCCT
PRIMER_LEFT_28=891,20
PRIMER_RIGHT_28=1695,23
PRIMER_LEFT_28_TM=61.748
PRIMER_RIGHT_28_TM=57.268
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=10.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=805
PRIMER_PAIR_29_PENALTY=7.498995
PRIMER_LEFT_29_PENALTY=1.748344
PRIMER_RIGHT_29_PENALTY=5.750651
PRIMER_LEFT_29_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_29_SEQUENCE=GAGTCTAAATCTCCCCTTTTTCTTC
PRIMER_LEFT_29=891,20
PRIMER_RIGHT_29=1689,25
PRIMER_LEFT_29_TM=61.748
PRIMER_RIGHT_29_TM=59.249
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=799
PRIMER_PAIR_30_PENALTY=7.506238
PRIMER_LEFT_30_PENALTY=4.022310
PRIMER_RIGHT_30_PENALTY=3.483928
PRIMER_LEFT_30_SEQUENCE=TGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_30_SEQUENCE=GTCTTTGCTTCGGTTTATGAGA
PRIMER_LEFT_30=801,20
PRIMER_RIGHT_30=1210,22
PRIMER_LEFT_30_TM=55.978
PRIMER_RIGHT_30_TM=58.516
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=410
PRIMER_PAIR_31_PENALTY=7.506238
PRIMER_LEFT_31_PENALTY=4.022310
PRIMER_RIGHT_31_PENALTY=3.483928
PRIMER_LEFT_31_SEQUENCE=GTGTTGAAGTGGAGAGCTTG
PRIMER_RIGHT_31_SEQUENCE=GTCTTTGCTTCGGTTTATGAGA
PRIMER_LEFT_31=800,20
PRIMER_RIGHT_31=1210,22
PRIMER_LEFT_31_TM=55.978
PRIMER_RIGHT_31_TM=58.516
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=411
PRIMER_PAIR_32_PENALTY=7.507770
PRIMER_LEFT_32_PENALTY=1.748344
PRIMER_RIGHT_32_PENALTY=5.759426
PRIMER_LEFT_32_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_32_SEQUENCE=TGAAAGAGTCTAAATCTCCCCT
PRIMER_LEFT_32=891,20
PRIMER_RIGHT_32=1694,22
PRIMER_LEFT_32_TM=61.748
PRIMER_RIGHT_32_TM=56.241
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=10.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=804
PRIMER_PAIR_33_PENALTY=7.507770
PRIMER_LEFT_33_PENALTY=1.748344
PRIMER_RIGHT_33_PENALTY=5.759426
PRIMER_LEFT_33_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_33_SEQUENCE=CTGAAAGAGTCTAAATCTCCCC
PRIMER_LEFT_33=891,20
PRIMER_RIGHT_33=1695,22
PRIMER_LEFT_33_TM=61.748
PRIMER_RIGHT_33_TM=56.241
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=10.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=805
PRIMER_PAIR_34_PENALTY=7.621011
PRIMER_LEFT_34_PENALTY=1.748344
PRIMER_RIGHT_34_PENALTY=5.872667
PRIMER_LEFT_34_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_34_SEQUENCE=ACTGAAAGAGTCTAAATCTCCCC
PRIMER_LEFT_34=891,20
PRIMER_RIGHT_34=1696,23
PRIMER_LEFT_34_TM=61.748
PRIMER_RIGHT_34_TM=57.127
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=43.478
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=10.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=806
PRIMER_PAIR_35_PENALTY=7.631511
PRIMER_LEFT_35_PENALTY=1.748344
PRIMER_RIGHT_35_PENALTY=5.883167
PRIMER_LEFT_35_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_35_SEQUENCE=TGAAAGAGTCTAAATCTCCCC
PRIMER_LEFT_35=891,20
PRIMER_RIGHT_35=1694,21
PRIMER_LEFT_35_TM=61.748
PRIMER_RIGHT_35_TM=55.117
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=10.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=804
PRIMER_PAIR_36_PENALTY=7.665406
PRIMER_LEFT_36_PENALTY=1.748344
PRIMER_RIGHT_36_PENALTY=5.917062
PRIMER_LEFT_36_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_36_SEQUENCE=ACTGAAAGAGTCTAAATCTCCCCT
PRIMER_LEFT_36=891,20
PRIMER_RIGHT_36=1696,24
PRIMER_LEFT_36_TM=61.748
PRIMER_RIGHT_36_TM=58.083
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=41.667
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=10.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=806
PRIMER_PAIR_37_PENALTY=7.717099
PRIMER_LEFT_37_PENALTY=1.748344
PRIMER_RIGHT_37_PENALTY=5.968755
PRIMER_LEFT_37_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_37_SEQUENCE=TTGGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_37=891,20
PRIMER_RIGHT_37=1779,24
PRIMER_LEFT_37_TM=61.748
PRIMER_RIGHT_37_TM=61.969
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.833
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=889
PRIMER_PAIR_38_PENALTY=7.781680
PRIMER_LEFT_38_PENALTY=3.984632
PRIMER_RIGHT_38_PENALTY=3.797048
PRIMER_LEFT_38_SEQUENCE=GTGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_38_SEQUENCE=TGGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_38=800,21
PRIMER_RIGHT_38=1778,23
PRIMER_LEFT_38_TM=57.015
PRIMER_RIGHT_38_TM=60.797
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=47.826
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=979
PRIMER_PAIR_39_PENALTY=7.805019
PRIMER_LEFT_39_PENALTY=1.748344
PRIMER_RIGHT_39_PENALTY=6.056675
PRIMER_LEFT_39_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_39_SEQUENCE=GCTCTTGGTACAACTCTATGTTTGA
PRIMER_LEFT_39=891,20
PRIMER_RIGHT_39=1535,25
PRIMER_LEFT_39_TM=61.748
PRIMER_RIGHT_39_TM=58.943
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=645
PRIMER_PAIR_40_PENALTY=7.819358
PRIMER_LEFT_40_PENALTY=4.022310
PRIMER_RIGHT_40_PENALTY=3.797048
PRIMER_LEFT_40_SEQUENCE=TGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_40_SEQUENCE=TGGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_40=801,20
PRIMER_RIGHT_40=1778,23
PRIMER_LEFT_40_TM=55.978
PRIMER_RIGHT_40_TM=60.797
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=47.826
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=978
PRIMER_PAIR_41_PENALTY=7.819358
PRIMER_LEFT_41_PENALTY=4.022310
PRIMER_RIGHT_41_PENALTY=3.797048
PRIMER_LEFT_41_SEQUENCE=GTGTTGAAGTGGAGAGCTTG
PRIMER_RIGHT_41_SEQUENCE=TGGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_41=800,20
PRIMER_RIGHT_41=1778,23
PRIMER_LEFT_41_TM=55.978
PRIMER_RIGHT_41_TM=60.797
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.826
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=979
PRIMER_PAIR_42_PENALTY=7.877646
PRIMER_LEFT_42_PENALTY=1.748344
PRIMER_RIGHT_42_PENALTY=6.129302
PRIMER_LEFT_42_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_42_SEQUENCE=ACCTCTCATCACTAAGCAGAAA
PRIMER_LEFT_42=891,20
PRIMER_RIGHT_42=1774,22
PRIMER_LEFT_42_TM=61.748
PRIMER_RIGHT_42_TM=55.871
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=884
PRIMER_PAIR_43_PENALTY=7.877646
PRIMER_LEFT_43_PENALTY=1.748344
PRIMER_RIGHT_43_PENALTY=6.129302
PRIMER_LEFT_43_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_43_SEQUENCE=AACCTCTCATCACTAAGCAGAA
PRIMER_LEFT_43=891,20
PRIMER_RIGHT_43=1775,22
PRIMER_LEFT_43_TM=61.748
PRIMER_RIGHT_43_TM=55.871
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=885
PRIMER_PAIR_44_PENALTY=7.900054
PRIMER_LEFT_44_PENALTY=1.748344
PRIMER_RIGHT_44_PENALTY=6.151710
PRIMER_LEFT_44_SEQUENCE=GAAACTGTTGCCGCATCTTG
PRIMER_RIGHT_44_SEQUENCE=GCTTTATTTGGAACCTCTCATCACT
PRIMER_LEFT_44=891,20
PRIMER_RIGHT_44=1786,25
PRIMER_LEFT_44_TM=61.748
PRIMER_RIGHT_44_TM=61.152
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=896
PRIMER_PAIR_45_PENALTY=7.902557
PRIMER_LEFT_45_PENALTY=3.984632
PRIMER_RIGHT_45_PENALTY=3.917925
PRIMER_LEFT_45_SEQUENCE=GTGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_45_SEQUENCE=GGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_45=800,21
PRIMER_RIGHT_45=1777,22
PRIMER_LEFT_45_TM=57.015
PRIMER_RIGHT_45_TM=58.082
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=978
PRIMER_PAIR_46_PENALTY=7.940235
PRIMER_LEFT_46_PENALTY=4.022310
PRIMER_RIGHT_46_PENALTY=3.917925
PRIMER_LEFT_46_SEQUENCE=TGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_46_SEQUENCE=GGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_46=801,20
PRIMER_RIGHT_46=1777,22
PRIMER_LEFT_46_TM=55.978
PRIMER_RIGHT_46_TM=58.082
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=977
PRIMER_PAIR_47_PENALTY=7.940235
PRIMER_LEFT_47_PENALTY=4.022310
PRIMER_RIGHT_47_PENALTY=3.917925
PRIMER_LEFT_47_SEQUENCE=GTGTTGAAGTGGAGAGCTTG
PRIMER_RIGHT_47_SEQUENCE=GGAACCTCTCATCACTAAGCAG
PRIMER_LEFT_47=800,20
PRIMER_RIGHT_47=1777,22
PRIMER_LEFT_47_TM=55.978
PRIMER_RIGHT_47_TM=58.082
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=978
PRIMER_PAIR_48_PENALTY=7.984674
PRIMER_LEFT_48_PENALTY=3.984632
PRIMER_RIGHT_48_PENALTY=4.000042
PRIMER_LEFT_48_SEQUENCE=GTGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_48_SEQUENCE=GGAACCTCTCATCACTAAGCA
PRIMER_LEFT_48=800,21
PRIMER_RIGHT_48=1777,21
PRIMER_LEFT_48_TM=57.015
PRIMER_RIGHT_48_TM=57.000
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=978
PRIMER_PAIR_49_PENALTY=8.022352
PRIMER_LEFT_49_PENALTY=4.022310
PRIMER_RIGHT_49_PENALTY=4.000042
PRIMER_LEFT_49_SEQUENCE=TGTTGAAGTGGAGAGCTTGT
PRIMER_RIGHT_49_SEQUENCE=GGAACCTCTCATCACTAAGCA
PRIMER_LEFT_49=801,20
PRIMER_RIGHT_49=1777,21
PRIMER_LEFT_49_TM=55.978
PRIMER_RIGHT_49_TM=57.000
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=977
=
SEQUENCE_ID=MAGIC.426_1_19414807_19414881_ler-0_oy-0_20291_390055_TRUE
SEQUENCE_TARGET=950,174
SEQUENCE_TEMPLATE=GGACACTCTCTGATCAACTGCTGACGGCGAATAGAACTCGGTTTAGGACAGTCATGATAAAGCCTCTCAAGTGCTTCAACTTGTTCAGGTGTATACCTCACATACTTCCCATTATCCAAACATCCCAACTTACCATCCTTGCAAGACATTGCCATTACTCCTCAGNAANANTCTTCTTATTTCACAATCACAGAATCTCACAAATGACCCNACTACCCCCTNCAGATGANTAACTAAATCCTTCTAAACAGAAGATAACCACAACATACTAAACAAAGGAGACAGAGAGCTTCTTAGTCCACCACAAACTCCACAGCTTGAACAACTACCAAATCTAAGCCATCTCAAATCCTAAGCACTTGGAGTAGCTTCACAAATCACATTACCGCATAAATATCGAATTCTGTTTCCTTCAAAACACACTAGAGGCTTTTTTTCTTCANGCAATCTCAAAAGGGCANACAAAGACAAAACCTATCCTTTATATTACTCCTCGNCTTTTANGAGCTAAATACCAAAACACAAGAAAGAAAAACAGACTTTAAGATTACAACTTTCCCTTTTACAAACACCGAAAAAAGATTCGACCTTTTCTCTGAACACACACACCTAAAAAAACAAGAAAGTTACAAACTTTTTTATATTTGCTCGTGACNANNACACAGAANCACCGAGNTGACTCAGCCATTAGAAACCCCGATTCCACCTCGAAACGAAGCCATAATATTATCAGTTCTCAAANCCATATTCTCCTTCTTCGAGCTCTCATTGAAATAAAAATGAAANCTTTAGAGNAAAGAAAAACCCTAGAAATTTANTCGAAGANNNNNNNAGGAAAGAGGAAAGTAGAAGAGAGGANGAAGAAGAGATGTATGTAGAAAACGAATCATGGAGCTACTGTACTGTTTTGTTGNTTTTAGATGTTATCTACTTTGTGTGTTTTTTTTCCTCTAGCTCGTTCATGGCAACTTTGNTTTNTTTTTTTGGCNNNNNNNNAATANTANNNNNTNAATAATGTGATTTGATAGTGCTGTTGCGTTTGTGAAATTANCTTTTTCGTTGGCATTTNNTTTTNNTTTTTTTTNTGATTGGCACATTTCAATTTCATAAACCAACAAACAACAACACCTCGAGGNAAAACCAAANTTCTCNTGTTTACTNTACGAAAATGGTCTAAATAGGNATAATTGTCAAAAAAANTGTTCCAAAAGGTTNAAAAAAGGNTAATTAAAGCNTAAAACATTCTCAAATATATAATCAGTGNANATTGTANACGAGAAAAAGGCNGTCAAATTGAAGAGAAAGNCAATCAAATGCTTATACTAGTGTAATAAATTTTNTTTTNTTGCTATTTGTGAAAATTACCTATTGTTATACTATANNNNTTTTNTTATACTNTAGTTTTTATATTTATAAANAAAAAAATTATGGTCATGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGTNATGCAATTTCTTNGATCTAATCATGGAAAACAACATAGATAATGTTAACNTTTAATGTTNTAATGTTTGTGACTAATNNCTAACGGATGCTAATCTTATACTCCTACATTTATGCCCACAATATGAAATTAATGAGGTTTATACCAATAAATATTANTACTTCTAAGTTTTAACTAGTAGTAAACGAGTGGTAAAAGAGTGAATAGGAGGGTAATAAAGGTAAGAAGGAATGGGGAAGGTCAGAGGCGTATAAAAAATAACAAAAGGCGTGGGAGAGAGGTCTGCGTAGGAGACAGAGACAACATCATTACCTTCTTTGCATGCGTCTCCTCCGTCTTAAAGCTGAAGCTTCACTTTCTTTNNTTCNTTCTTTCTTTTTCTCTATTCTCAAATTTCTTTNTTTTATTTTGTCAACTCTNTTTCAAATATTTTAATTTAACTAGTACTATTCCTTCATTTTCCCAATAATGCCTGTAAAAGAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=5.748982
PRIMER_LEFT_0_PENALTY=2.265053
PRIMER_RIGHT_0_PENALTY=3.483928
PRIMER_LEFT_0_SEQUENCE=TCTCCTTCTTCGAGCTCTCATT
PRIMER_RIGHT_0_SEQUENCE=TTGTGGGCATAAATGTAGGAGT
PRIMER_LEFT_0=748,22
PRIMER_RIGHT_0=1712,22
PRIMER_LEFT_0_TM=59.735
PRIMER_RIGHT_0_TM=58.516
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=965
PRIMER_PAIR_1_PENALTY=5.838922
PRIMER_LEFT_1_PENALTY=5.430521
PRIMER_RIGHT_1_PENALTY=0.408401
PRIMER_LEFT_1_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_1_SEQUENCE=TACGCAGACCTCTCTCCCAC
PRIMER_LEFT_1=889,24
PRIMER_RIGHT_1=1879,20
PRIMER_LEFT_1_TM=58.569
PRIMER_RIGHT_1_TM=60.408
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=991
PRIMER_PAIR_2_PENALTY=5.877172
PRIMER_LEFT_2_PENALTY=5.430521
PRIMER_RIGHT_2_PENALTY=0.446651
PRIMER_LEFT_2_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_2_SEQUENCE=CTCTGACCTTCCCCATTCCT
PRIMER_LEFT_2=889,24
PRIMER_RIGHT_2=1836,20
PRIMER_LEFT_2_TM=58.569
PRIMER_RIGHT_2_TM=60.447
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=948
PRIMER_PAIR_3_PENALTY=5.885488
PRIMER_LEFT_3_PENALTY=5.477086
PRIMER_RIGHT_3_PENALTY=0.408401
PRIMER_LEFT_3_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_3_SEQUENCE=TACGCAGACCTCTCTCCCAC
PRIMER_LEFT_3=886,24
PRIMER_RIGHT_3=1879,20
PRIMER_LEFT_3_TM=58.523
PRIMER_RIGHT_3_TM=60.408
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=994
PRIMER_PAIR_4_PENALTY=5.923737
PRIMER_LEFT_4_PENALTY=5.477086
PRIMER_RIGHT_4_PENALTY=0.446651
PRIMER_LEFT_4_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_4_SEQUENCE=CTCTGACCTTCCCCATTCCT
PRIMER_LEFT_4=886,24
PRIMER_RIGHT_4=1836,20
PRIMER_LEFT_4_TM=58.523
PRIMER_RIGHT_4_TM=60.447
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=951
PRIMER_PAIR_5_PENALTY=5.975652
PRIMER_LEFT_5_PENALTY=5.430521
PRIMER_RIGHT_5_PENALTY=0.545130
PRIMER_LEFT_5_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_5_SEQUENCE=CTACGCAGACCTCTCTCCCA
PRIMER_LEFT_5=889,24
PRIMER_RIGHT_5=1880,20
PRIMER_LEFT_5_TM=58.569
PRIMER_RIGHT_5_TM=60.545
PRIMER_LEFT_5_GC_PERCENT=41.667
PRIMER_RIGHT_5_GC_PERCENT=60.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=992
PRIMER_PAIR_6_PENALTY=5.979058
PRIMER_LEFT_6_PENALTY=5.430521
PRIMER_RIGHT_6_PENALTY=0.548537
PRIMER_LEFT_6_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_6_SEQUENCE=TCCCACGCCTTTTGTTATTT
PRIMER_LEFT_6=889,24
PRIMER_RIGHT_6=1865,20
PRIMER_LEFT_6_TM=58.569
PRIMER_RIGHT_6_TM=59.451
PRIMER_LEFT_6_GC_PERCENT=41.667
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=977
PRIMER_PAIR_7_PENALTY=6.022217
PRIMER_LEFT_7_PENALTY=5.477086
PRIMER_RIGHT_7_PENALTY=0.545130
PRIMER_LEFT_7_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_7_SEQUENCE=CTACGCAGACCTCTCTCCCA
PRIMER_LEFT_7=886,24
PRIMER_RIGHT_7=1880,20
PRIMER_LEFT_7_TM=58.523
PRIMER_RIGHT_7_TM=60.545
PRIMER_LEFT_7_GC_PERCENT=41.667
PRIMER_RIGHT_7_GC_PERCENT=60.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=995
PRIMER_PAIR_8_PENALTY=6.025623
PRIMER_LEFT_8_PENALTY=5.477086
PRIMER_RIGHT_8_PENALTY=0.548537
PRIMER_LEFT_8_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_8_SEQUENCE=TCCCACGCCTTTTGTTATTT
PRIMER_LEFT_8=886,24
PRIMER_RIGHT_8=1865,20
PRIMER_LEFT_8_TM=58.523
PRIMER_RIGHT_8_TM=59.451
PRIMER_LEFT_8_GC_PERCENT=41.667
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=6.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=980
PRIMER_PAIR_9_PENALTY=6.057364
PRIMER_LEFT_9_PENALTY=5.430521
PRIMER_RIGHT_9_PENALTY=0.626843
PRIMER_LEFT_9_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_9_SEQUENCE=GACCTTCCCCATTCCTTCTT
PRIMER_LEFT_9=889,24
PRIMER_RIGHT_9=1832,20
PRIMER_LEFT_9_TM=58.569
PRIMER_RIGHT_9_TM=59.373
PRIMER_LEFT_9_GC_PERCENT=41.667
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=944
PRIMER_PAIR_10_PENALTY=6.094646
PRIMER_LEFT_10_PENALTY=5.686244
PRIMER_RIGHT_10_PENALTY=0.408401
PRIMER_LEFT_10_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_10_SEQUENCE=TACGCAGACCTCTCTCCCAC
PRIMER_LEFT_10=886,25
PRIMER_RIGHT_10=1879,20
PRIMER_LEFT_10_TM=59.314
PRIMER_RIGHT_10_TM=60.408
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=60.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=994
PRIMER_PAIR_11_PENALTY=6.103930
PRIMER_LEFT_11_PENALTY=5.477086
PRIMER_RIGHT_11_PENALTY=0.626843
PRIMER_LEFT_11_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_11_SEQUENCE=GACCTTCCCCATTCCTTCTT
PRIMER_LEFT_11=886,24
PRIMER_RIGHT_11=1832,20
PRIMER_LEFT_11_TM=58.523
PRIMER_RIGHT_11_TM=59.373
PRIMER_LEFT_11_GC_PERCENT=41.667
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=947
PRIMER_PAIR_12_PENALTY=6.132895
PRIMER_LEFT_12_PENALTY=5.686244
PRIMER_RIGHT_12_PENALTY=0.446651
PRIMER_LEFT_12_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_12_SEQUENCE=CTCTGACCTTCCCCATTCCT
PRIMER_LEFT_12=886,25
PRIMER_RIGHT_12=1836,20
PRIMER_LEFT_12_TM=59.314
PRIMER_RIGHT_12_TM=60.447
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=951
PRIMER_PAIR_13_PENALTY=6.231375
PRIMER_LEFT_13_PENALTY=5.686244
PRIMER_RIGHT_13_PENALTY=0.545130
PRIMER_LEFT_13_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_13_SEQUENCE=CTACGCAGACCTCTCTCCCA
PRIMER_LEFT_13=886,25
PRIMER_RIGHT_13=1880,20
PRIMER_LEFT_13_TM=59.314
PRIMER_RIGHT_13_TM=60.545
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=60.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=995
PRIMER_PAIR_14_PENALTY=6.234781
PRIMER_LEFT_14_PENALTY=5.686244
PRIMER_RIGHT_14_PENALTY=0.548537
PRIMER_LEFT_14_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_14_SEQUENCE=TCCCACGCCTTTTGTTATTT
PRIMER_LEFT_14=886,25
PRIMER_RIGHT_14=1865,20
PRIMER_LEFT_14_TM=59.314
PRIMER_RIGHT_14_TM=59.451
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=980
PRIMER_PAIR_15_PENALTY=6.252449
PRIMER_LEFT_15_PENALTY=5.430521
PRIMER_RIGHT_15_PENALTY=0.821928
PRIMER_LEFT_15_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_15_SEQUENCE=TGACCTTCCCCATTCCTTCT
PRIMER_LEFT_15=889,24
PRIMER_RIGHT_15=1833,20
PRIMER_LEFT_15_TM=58.569
PRIMER_RIGHT_15_TM=60.822
PRIMER_LEFT_15_GC_PERCENT=41.667
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=945
PRIMER_PAIR_16_PENALTY=6.252449
PRIMER_LEFT_16_PENALTY=5.430521
PRIMER_RIGHT_16_PENALTY=0.821928
PRIMER_LEFT_16_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_16_SEQUENCE=CTGACCTTCCCCATTCCTTC
PRIMER_LEFT_16=889,24
PRIMER_RIGHT_16=1834,20
PRIMER_LEFT_16_TM=58.569
PRIMER_RIGHT_16_TM=60.822
PRIMER_LEFT_16_GC_PERCENT=41.667
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=946
PRIMER_PAIR_17_PENALTY=6.252449
PRIMER_LEFT_17_PENALTY=5.430521
PRIMER_RIGHT_17_PENALTY=0.821928
PRIMER_LEFT_17_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_17_SEQUENCE=TCTGACCTTCCCCATTCCTT
PRIMER_LEFT_17=889,24
PRIMER_RIGHT_17=1835,20
PRIMER_LEFT_17_TM=58.569
PRIMER_RIGHT_17_TM=60.822
PRIMER_LEFT_17_GC_PERCENT=41.667
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=947
PRIMER_PAIR_18_PENALTY=6.280609
PRIMER_LEFT_18_PENALTY=5.430521
PRIMER_RIGHT_18_PENALTY=0.850088
PRIMER_LEFT_18_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_18_SEQUENCE=TATACGCCTCTGACCTTCCC
PRIMER_LEFT_18=889,24
PRIMER_RIGHT_18=1843,20
PRIMER_LEFT_18_TM=58.569
PRIMER_RIGHT_18_TM=59.150
PRIMER_LEFT_18_GC_PERCENT=41.667
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=955
PRIMER_PAIR_19_PENALTY=6.299014
PRIMER_LEFT_19_PENALTY=5.477086
PRIMER_RIGHT_19_PENALTY=0.821928
PRIMER_LEFT_19_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_19_SEQUENCE=TGACCTTCCCCATTCCTTCT
PRIMER_LEFT_19=886,24
PRIMER_RIGHT_19=1833,20
PRIMER_LEFT_19_TM=58.523
PRIMER_RIGHT_19_TM=60.822
PRIMER_LEFT_19_GC_PERCENT=41.667
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=948
PRIMER_PAIR_20_PENALTY=6.299014
PRIMER_LEFT_20_PENALTY=5.477086
PRIMER_RIGHT_20_PENALTY=0.821928
PRIMER_LEFT_20_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_20_SEQUENCE=CTGACCTTCCCCATTCCTTC
PRIMER_LEFT_20=886,24
PRIMER_RIGHT_20=1834,20
PRIMER_LEFT_20_TM=58.523
PRIMER_RIGHT_20_TM=60.822
PRIMER_LEFT_20_GC_PERCENT=41.667
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=949
PRIMER_PAIR_21_PENALTY=6.299014
PRIMER_LEFT_21_PENALTY=5.477086
PRIMER_RIGHT_21_PENALTY=0.821928
PRIMER_LEFT_21_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_21_SEQUENCE=TCTGACCTTCCCCATTCCTT
PRIMER_LEFT_21=886,24
PRIMER_RIGHT_21=1835,20
PRIMER_LEFT_21_TM=58.523
PRIMER_RIGHT_21_TM=60.822
PRIMER_LEFT_21_GC_PERCENT=41.667
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=950
PRIMER_PAIR_22_PENALTY=6.313088
PRIMER_LEFT_22_PENALTY=5.686244
PRIMER_RIGHT_22_PENALTY=0.626843
PRIMER_LEFT_22_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_22_SEQUENCE=GACCTTCCCCATTCCTTCTT
PRIMER_LEFT_22=886,25
PRIMER_RIGHT_22=1832,20
PRIMER_LEFT_22_TM=59.314
PRIMER_RIGHT_22_TM=59.373
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=947
PRIMER_PAIR_23_PENALTY=6.327174
PRIMER_LEFT_23_PENALTY=5.477086
PRIMER_RIGHT_23_PENALTY=0.850088
PRIMER_LEFT_23_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_23_SEQUENCE=TATACGCCTCTGACCTTCCC
PRIMER_LEFT_23=886,24
PRIMER_RIGHT_23=1843,20
PRIMER_LEFT_23_TM=58.523
PRIMER_RIGHT_23_TM=59.150
PRIMER_LEFT_23_GC_PERCENT=41.667
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=958
PRIMER_PAIR_24_PENALTY=6.343087
PRIMER_LEFT_24_PENALTY=5.430521
PRIMER_RIGHT_24_PENALTY=0.912566
PRIMER_LEFT_24_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_24_SEQUENCE=CTCCCACGCCTTTTGTTATT
PRIMER_LEFT_24=889,24
PRIMER_RIGHT_24=1866,20
PRIMER_LEFT_24_TM=58.569
PRIMER_RIGHT_24_TM=59.087
PRIMER_LEFT_24_GC_PERCENT=41.667
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=978
PRIMER_PAIR_25_PENALTY=6.389652
PRIMER_LEFT_25_PENALTY=5.477086
PRIMER_RIGHT_25_PENALTY=0.912566
PRIMER_LEFT_25_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_25_SEQUENCE=CTCCCACGCCTTTTGTTATT
PRIMER_LEFT_25=886,24
PRIMER_RIGHT_25=1866,20
PRIMER_LEFT_25_TM=58.523
PRIMER_RIGHT_25_TM=59.087
PRIMER_LEFT_25_GC_PERCENT=41.667
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=6.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=981
PRIMER_PAIR_26_PENALTY=6.443951
PRIMER_LEFT_26_PENALTY=5.430521
PRIMER_RIGHT_26_PENALTY=1.013430
PRIMER_LEFT_26_SEQUENCE=TGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_26_SEQUENCE=CCCACGCCTTTTGTTATTTT
PRIMER_LEFT_26=889,24
PRIMER_RIGHT_26=1864,20
PRIMER_LEFT_26_TM=58.569
PRIMER_RIGHT_26_TM=58.987
PRIMER_LEFT_26_GC_PERCENT=41.667
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=976
PRIMER_PAIR_27_PENALTY=6.490516
PRIMER_LEFT_27_PENALTY=5.477086
PRIMER_RIGHT_27_PENALTY=1.013430
PRIMER_LEFT_27_SEQUENCE=TCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_27_SEQUENCE=CCCACGCCTTTTGTTATTTT
PRIMER_LEFT_27=886,24
PRIMER_RIGHT_27=1864,20
PRIMER_LEFT_27_TM=58.523
PRIMER_RIGHT_27_TM=58.987
PRIMER_LEFT_27_GC_PERCENT=41.667
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=7.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=979
PRIMER_PAIR_28_PENALTY=6.508172
PRIMER_LEFT_28_PENALTY=5.686244
PRIMER_RIGHT_28_PENALTY=0.821928
PRIMER_LEFT_28_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_28_SEQUENCE=TGACCTTCCCCATTCCTTCT
PRIMER_LEFT_28=886,25
PRIMER_RIGHT_28=1833,20
PRIMER_LEFT_28_TM=59.314
PRIMER_RIGHT_28_TM=60.822
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=948
PRIMER_PAIR_29_PENALTY=6.508172
PRIMER_LEFT_29_PENALTY=5.686244
PRIMER_RIGHT_29_PENALTY=0.821928
PRIMER_LEFT_29_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_29_SEQUENCE=CTGACCTTCCCCATTCCTTC
PRIMER_LEFT_29=886,25
PRIMER_RIGHT_29=1834,20
PRIMER_LEFT_29_TM=59.314
PRIMER_RIGHT_29_TM=60.822
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=949
PRIMER_PAIR_30_PENALTY=6.508172
PRIMER_LEFT_30_PENALTY=5.686244
PRIMER_RIGHT_30_PENALTY=0.821928
PRIMER_LEFT_30_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_30_SEQUENCE=TCTGACCTTCCCCATTCCTT
PRIMER_LEFT_30=886,25
PRIMER_RIGHT_30=1835,20
PRIMER_LEFT_30_TM=59.314
PRIMER_RIGHT_30_TM=60.822
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=950
PRIMER_PAIR_31_PENALTY=6.528484
PRIMER_LEFT_31_PENALTY=6.120083
PRIMER_RIGHT_31_PENALTY=0.408401
PRIMER_LEFT_31_SEQUENCE=ATGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_31_SEQUENCE=TACGCAGACCTCTCTCCCAC
PRIMER_LEFT_31=888,25
PRIMER_RIGHT_31=1879,20
PRIMER_LEFT_31_TM=58.880
PRIMER_RIGHT_31_TM=60.408
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=60.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=992
PRIMER_PAIR_32_PENALTY=6.528484
PRIMER_LEFT_32_PENALTY=6.120083
PRIMER_RIGHT_32_PENALTY=0.408401
PRIMER_LEFT_32_SEQUENCE=CATGGAGCTACTGTACTGTTTTGTT
PRIMER_RIGHT_32_SEQUENCE=TACGCAGACCTCTCTCCCAC
PRIMER_LEFT_32=887,25
PRIMER_RIGHT_32=1879,20
PRIMER_LEFT_32_TM=58.880
PRIMER_RIGHT_32_TM=60.408
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=60.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=993
PRIMER_PAIR_33_PENALTY=6.536332
PRIMER_LEFT_33_PENALTY=5.686244
PRIMER_RIGHT_33_PENALTY=0.850088
PRIMER_LEFT_33_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_33_SEQUENCE=TATACGCCTCTGACCTTCCC
PRIMER_LEFT_33=886,25
PRIMER_RIGHT_33=1843,20
PRIMER_LEFT_33_TM=59.314
PRIMER_RIGHT_33_TM=59.150
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=958
PRIMER_PAIR_34_PENALTY=6.566734
PRIMER_LEFT_34_PENALTY=6.120083
PRIMER_RIGHT_34_PENALTY=0.446651
PRIMER_LEFT_34_SEQUENCE=ATGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_34_SEQUENCE=CTCTGACCTTCCCCATTCCT
PRIMER_LEFT_34=888,25
PRIMER_RIGHT_34=1836,20
PRIMER_LEFT_34_TM=58.880
PRIMER_RIGHT_34_TM=60.447
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=949
PRIMER_PAIR_35_PENALTY=6.566734
PRIMER_LEFT_35_PENALTY=6.120083
PRIMER_RIGHT_35_PENALTY=0.446651
PRIMER_LEFT_35_SEQUENCE=CATGGAGCTACTGTACTGTTTTGTT
PRIMER_RIGHT_35_SEQUENCE=CTCTGACCTTCCCCATTCCT
PRIMER_LEFT_35=887,25
PRIMER_RIGHT_35=1836,20
PRIMER_LEFT_35_TM=58.880
PRIMER_RIGHT_35_TM=60.447
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=950
PRIMER_PAIR_36_PENALTY=6.570382
PRIMER_LEFT_36_PENALTY=6.161980
PRIMER_RIGHT_36_PENALTY=0.408401
PRIMER_LEFT_36_SEQUENCE=ATCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_36_SEQUENCE=TACGCAGACCTCTCTCCCAC
PRIMER_LEFT_36=885,25
PRIMER_RIGHT_36=1879,20
PRIMER_LEFT_36_TM=58.838
PRIMER_RIGHT_36_TM=60.408
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=60.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=995
PRIMER_PAIR_37_PENALTY=6.588983
PRIMER_LEFT_37_PENALTY=3.105055
PRIMER_RIGHT_37_PENALTY=3.483928
PRIMER_LEFT_37_SEQUENCE=CTCCTTCTTCGAGCTCTCATT
PRIMER_RIGHT_37_SEQUENCE=TTGTGGGCATAAATGTAGGAGT
PRIMER_LEFT_37=749,21
PRIMER_RIGHT_37=1712,22
PRIMER_LEFT_37_TM=57.895
PRIMER_RIGHT_37_TM=58.516
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=964
PRIMER_PAIR_38_PENALTY=6.598810
PRIMER_LEFT_38_PENALTY=5.686244
PRIMER_RIGHT_38_PENALTY=0.912566
PRIMER_LEFT_38_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_38_SEQUENCE=CTCCCACGCCTTTTGTTATT
PRIMER_LEFT_38=886,25
PRIMER_RIGHT_38=1866,20
PRIMER_LEFT_38_TM=59.314
PRIMER_RIGHT_38_TM=59.087
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=981
PRIMER_PAIR_39_PENALTY=6.608631
PRIMER_LEFT_39_PENALTY=6.161980
PRIMER_RIGHT_39_PENALTY=0.446651
PRIMER_LEFT_39_SEQUENCE=ATCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_39_SEQUENCE=CTCTGACCTTCCCCATTCCT
PRIMER_LEFT_39=885,25
PRIMER_RIGHT_39=1836,20
PRIMER_LEFT_39_TM=58.838
PRIMER_RIGHT_39_TM=60.447
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=952
PRIMER_PAIR_40_PENALTY=6.665214
PRIMER_LEFT_40_PENALTY=6.120083
PRIMER_RIGHT_40_PENALTY=0.545130
PRIMER_LEFT_40_SEQUENCE=ATGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_40_SEQUENCE=CTACGCAGACCTCTCTCCCA
PRIMER_LEFT_40=888,25
PRIMER_RIGHT_40=1880,20
PRIMER_LEFT_40_TM=58.880
PRIMER_RIGHT_40_TM=60.545
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=60.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=993
PRIMER_PAIR_41_PENALTY=6.665214
PRIMER_LEFT_41_PENALTY=6.120083
PRIMER_RIGHT_41_PENALTY=0.545130
PRIMER_LEFT_41_SEQUENCE=CATGGAGCTACTGTACTGTTTTGTT
PRIMER_RIGHT_41_SEQUENCE=CTACGCAGACCTCTCTCCCA
PRIMER_LEFT_41=887,25
PRIMER_RIGHT_41=1880,20
PRIMER_LEFT_41_TM=58.880
PRIMER_RIGHT_41_TM=60.545
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=60.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=994
PRIMER_PAIR_42_PENALTY=6.668620
PRIMER_LEFT_42_PENALTY=6.120083
PRIMER_RIGHT_42_PENALTY=0.548537
PRIMER_LEFT_42_SEQUENCE=ATGGAGCTACTGTACTGTTTTGTTG
PRIMER_RIGHT_42_SEQUENCE=TCCCACGCCTTTTGTTATTT
PRIMER_LEFT_42=888,25
PRIMER_RIGHT_42=1865,20
PRIMER_LEFT_42_TM=58.880
PRIMER_RIGHT_42_TM=59.451
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=978
PRIMER_PAIR_43_PENALTY=6.668620
PRIMER_LEFT_43_PENALTY=6.120083
PRIMER_RIGHT_43_PENALTY=0.548537
PRIMER_LEFT_43_SEQUENCE=CATGGAGCTACTGTACTGTTTTGTT
PRIMER_RIGHT_43_SEQUENCE=TCCCACGCCTTTTGTTATTT
PRIMER_LEFT_43=887,25
PRIMER_RIGHT_43=1865,20
PRIMER_LEFT_43_TM=58.880
PRIMER_RIGHT_43_TM=59.451
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.2000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=979
PRIMER_PAIR_44_PENALTY=6.690287
PRIMER_LEFT_44_PENALTY=6.281886
PRIMER_RIGHT_44_PENALTY=0.408401
PRIMER_LEFT_44_SEQUENCE=CATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_44_SEQUENCE=TACGCAGACCTCTCTCCCAC
PRIMER_LEFT_44=887,23
PRIMER_RIGHT_44=1879,20
PRIMER_LEFT_44_TM=56.718
PRIMER_RIGHT_44_TM=60.408
PRIMER_LEFT_44_GC_PERCENT=43.478
PRIMER_RIGHT_44_GC_PERCENT=60.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=993
PRIMER_PAIR_45_PENALTY=6.691356
PRIMER_LEFT_45_PENALTY=3.207428
PRIMER_RIGHT_45_PENALTY=3.483928
PRIMER_LEFT_45_SEQUENCE=TCCTTCTTCGAGCTCTCATT
PRIMER_RIGHT_45_SEQUENCE=TTGTGGGCATAAATGTAGGAGT
PRIMER_LEFT_45=750,20
PRIMER_RIGHT_45=1712,22
PRIMER_LEFT_45_TM=56.793
PRIMER_RIGHT_45_TM=58.516
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=963
PRIMER_PAIR_46_PENALTY=6.699674
PRIMER_LEFT_46_PENALTY=5.686244
PRIMER_RIGHT_46_PENALTY=1.013430
PRIMER_LEFT_46_SEQUENCE=TCATGGAGCTACTGTACTGTTTTGT
PRIMER_RIGHT_46_SEQUENCE=CCCACGCCTTTTGTTATTTT
PRIMER_LEFT_46=886,25
PRIMER_RIGHT_46=1864,20
PRIMER_LEFT_46_TM=59.314
PRIMER_RIGHT_46_TM=58.987
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=979
PRIMER_PAIR_47_PENALTY=6.707111
PRIMER_LEFT_47_PENALTY=6.161980
PRIMER_RIGHT_47_PENALTY=0.545130
PRIMER_LEFT_47_SEQUENCE=ATCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_47_SEQUENCE=CTACGCAGACCTCTCTCCCA
PRIMER_LEFT_47=885,25
PRIMER_RIGHT_47=1880,20
PRIMER_LEFT_47_TM=58.838
PRIMER_RIGHT_47_TM=60.545
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=60.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=996
PRIMER_PAIR_48_PENALTY=6.710517
PRIMER_LEFT_48_PENALTY=6.161980
PRIMER_RIGHT_48_PENALTY=0.548537
PRIMER_LEFT_48_SEQUENCE=ATCATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_48_SEQUENCE=TCCCACGCCTTTTGTTATTT
PRIMER_LEFT_48=885,25
PRIMER_RIGHT_48=1865,20
PRIMER_LEFT_48_TM=58.838
PRIMER_RIGHT_48_TM=59.451
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=6.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=981
PRIMER_PAIR_49_PENALTY=6.728537
PRIMER_LEFT_49_PENALTY=6.281886
PRIMER_RIGHT_49_PENALTY=0.446651
PRIMER_LEFT_49_SEQUENCE=CATGGAGCTACTGTACTGTTTTG
PRIMER_RIGHT_49_SEQUENCE=CTCTGACCTTCCCCATTCCT
PRIMER_LEFT_49=887,23
PRIMER_RIGHT_49=1836,20
PRIMER_LEFT_49_TM=56.718
PRIMER_RIGHT_49_TM=60.447
PRIMER_LEFT_49_GC_PERCENT=43.478
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=950
=
SEQUENCE_ID=MAGIC.287_3_14255490_14255694_ct-1_rsch-4_216243_14856_TRUE
SEQUENCE_TARGET=950,304
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTATANGAAGAGAACTNNCANNANTACNGAGGCNGACNTTTTNANCTAATTTNCTANGTGTTGCACANACATTCCNTNTCGAAAAACGTGTTCAAGCGGTTATNNTCCAGTNAAGCTCCNGCAANTCTNGAAAANNGTGTCNAAGNTACCGTTTACCNNAAACGNGNNCGNTTTNTCTNNAAANCNTGTCGAAGNCACGATTCACACATGCATGNTTTCNATTCGCCNNGAAAAACATGNNAAAGTTANTGTTCANCCNCAACGNGTCNNNTTTGTCTCNNAAAANGTGTCNAAGCCACGNTTCACNCNNNNATGTTTNNNTTNGNCNCNAAAAACNTNCCAAAGNTANTGTTCACNCGNAACNGGCCNGGTTTGTCTNGAAAAANGTGTNNAAGCCACNATTCANTNANACATGTTTNNGTTCGNCTCNAAAAANGTGNCNAAGTTANTGTNCACNCGCAACNNGNNNNGTTTGNCTCGNAAAANGTGCCANAATCACTNAAACCATTNTCTGTNNTGTGCCTAACTAANATATCNAAATTCTTGAGANACGNCNGTCAAGNTNAANAAGACTCTNGCACAACGGANTNNNNGGNGGGGNNTTATTGTTGGACATNGGCCNTGCCNCTTCAATGTTAGGAGGCCCAAAACANATATCAANAAAGAAGAGGNCNTTTTGCCANTAAAAAGGNCCANTANGTCGAGAAGGGCAAAATNNTAATTTNCAGAGGAGACNNANNGNAATTTANGGTTCAGATTCCNTATAAATAGAGAGAAACNCNATTGGGAGCAGNATCCGATCCNNAGGTTATTAGCTGAGAGACAAANANAATNGTTGCCTTNCCCGAAATCNTTNCCTAGCCCGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATCGGTGCCTCNCCCATTTCNNTACTTTNTCTTTCTNCTTTTACGATTAAGTTCTCNGCTCNGNANGATAGTNTATAANCGNNGAGACNAAACTAGTTTNCTGTAATCTCTNGATCTTNGTTATCGTTAATAAAAANGACCAGNTCCGTTCACCCCCCAAAATCTCGTATATTATTATTGTTGCATNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_15069164_15069222_ws-0_zu-0_461760_783580_TRUE
SEQUENCE_TARGET=950,158
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_1291834_1292028_ler-0_tsu-0_46913_25063_TRUE
SEQUENCE_TARGET=950,294
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNATTGGAGNAANTGACNCTCAAANNNTNATTTTGNNGNTGTNCNCCCNTNAAAATNNNGGCCATTGTTGGANATNTTCNANNAAGANGAAATAANGTGAGATGNCATNGGGAACNNTGNCTAGANNAACNTTCAANACACTCNNNNNNNNTTTNATTATNNNNNTGGTNNNNNNNNNNNNTTTATNATTGGTGTCTCGTNTTTTTCATTTCACANAGTTATTAGGGACCTNCCACACTTATCTTTANTNGTCACAAACTGTGATTAATTCTNTANATGAACAACACANNNNNNNNNNNNNNNAGTTATGNNCNAAAAAAATAAATNTTNGTTGGTGAAATAAANTTANTTTATAAAAATCACTTGTTTNCTTTTTANTTTGAACGTGTGTTTNTNTTTTTTNNNCTTTTNTCTCTTATTATTTNAGTTACTTAATATAATANNNNNNNNNTATTTTTATNTAATAAATTAATATTTAANTTTTCTTATATTTTAAGATTTNTNCGTTGTNTTTTTTAAGTAAATAATTATATANAAAATAAANGAAATATCTAATATTAANTAACTAAAATGGAGAAGAAAANGANANAGANANAGAGAGAAAGCATGCCAAAGNGAGNGAAAGAGANNGATGAAATTGAGAGGGGCAAATNCNTGTTANTAANTATATTACCGTAAAACAAATGAAAATCGAAAGAAGTGTCTCTTTTTGCAAACAATGTTGGCCAAAGTTTGNNNTTAAGGTGTACTAGTTGGGTCAAAGAGTTGTTATTTGTTCCTTTGGGTCGACATAGAGTCGGGTTTAGTTTTGTTAATTGAATAATTCTAAGCCTCAAAAAAATCTTAAGAATTAAACTGTCTTCTTCTTCGGTCCCAGCCAAAAATCCAGAAGATTCTTAAATCTCATTTCTGCGGCGTAGGTGAAGTCTTAGCGATAAGCTTTGNTTCTTCTTCTTCTTCTTCTTTACCCATGGATTTTTAACGAACAAGAGAAGAGAAGNACCAAGTTTTCTTCGAAAGGATTGAACTTTGTTTCTTTCTCTTCANCTNCAANNNNCAAAAAATGATCAAGCTTTGCTTCATGACTTCTCATGGNTATTCAATCCCTGGCCTTGGTCTTCCTCAAGACCTCTGCAACACCGAAATCATCAAGGTAACTAATTCTTCAGAGTNNTCATTTCATTTTCATNTTGGATTTTTGATAATTTGGTTGCTNCTGTTATCTGGGTTCNTGTAAAATTGGTGTCTTTCGTCTACATCTGGTTGAATTTCATG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_1170619_1170627_ler-0_zu-0_38873_69004_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=TACAAAGATTAGTCAAGAAAATTCACCAATCTTCTTCTATCTCAAGAACATGATAACAATAGATGAGACCAAGAACACCGTTTAGTACTTTTCCAAATTTGTAGANAAGTTATGTCTCTCTTGTTTTGTAACCNTGTGACTNNNNNNNGTGAGTACTCTTGTGATATAGTNAGTGGTTATAGAAGAANAAGTTGTATGAATGTCATGTCTATCTTNTTTTGTAAANTTTCCCTATTGTATGTNTGNCCCAATATATAAAACCGGTGAATTTGTGATATAATGAGAAGTGGTTCNAGAAGACAAAGTTGGATAATTTCATTTCATACACTATACAACTAATAAAATGGTCAAGTTTTCATTGACATANCAATCAACATCGAGTCTTTTTAAGTCCTACCTAATACCCCCAAAAGATTTCAGGTTCTNAACAAGAGAAAGCAAGGATAATATCAAATGAAGATGCTTCTATTTTAGNCNTNNNNNNNNNNNNTTACGTGCAGCNTGTCACTTTTATGTTTTAAGTCCAAACCNCATTANANTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTCACTTTATCGAAGAAAAAAANNTTTNCNAGANTATCTTCAATTCNAAATCTGCAATTAATCTCCAAATCCACTCAACNCGATNNCTTTCTTTAAGATTGTTAATGGGTAAATAAGTAAAAGTTNTAATAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCNCCGCAATTTTAAATGGAGAAGNCANTTTAAATAAGCAAAATAAANNACAATCTTAAAGAAACCAAGACCTCNTATTCTCCAATTANTNAAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTANACTAATGCTAACNTTTTNTTTNNTTNTTCATTTTGATANNTTTTTATTTTATAATTTATAAACAAATACTTGAACAAGATTCTTTGAGTAAAATACTTTAGCCAATCTAAATCAAAAATATTTCAGCAAAACTGTTGTTNCCAANCATTTCTCTAAAATCGATACCAAAAATCAAANGANNNAAAAAAAAAAAACATTTTGTAAATTGAAAGTTGAAACTGTGCCTTGGTTGAAAAGTAAGAACGNNGCGTGTAGTTANACGCTTCNACNTGGCCCAACNNGTTCACATATTTTAGATTTGGTCGTTGTTGGTGTTTGGTAACATGTCCTGGCCCACCACCAANTAATTCTATAATTGNNNNNNTGNNTCTTATCTNNCTATCANNATCCTAAATTGCAACTTGCTGACGCTTTACCGGNCCAACGTAACTTTACTACACCTTGGTAACGTGTCTAATAAGCTGTATTTAGCAAAATTTTACCATGTAATATGTGGTTTACTTTTTTGGCATCATTTGTTT
PRIMER_LEFT_NUM_RETURNED=34
PRIMER_RIGHT_NUM_RETURNED=34
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=34
PRIMER_PAIR_0_PENALTY=7.510173
PRIMER_LEFT_0_PENALTY=7.232188
PRIMER_RIGHT_0_PENALTY=0.277985
PRIMER_LEFT_0_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_0_SEQUENCE=TCAACCAAGGCACAGTTTCA
PRIMER_LEFT_0=749,25
PRIMER_RIGHT_0=1719,20
PRIMER_LEFT_0_TM=62.232
PRIMER_RIGHT_0_TM=60.278
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=971
PRIMER_PAIR_1_PENALTY=8.371604
PRIMER_LEFT_1_PENALTY=7.232188
PRIMER_RIGHT_1_PENALTY=1.139416
PRIMER_LEFT_1_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_1_SEQUENCE=TTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_1=749,25
PRIMER_RIGHT_1=1721,21
PRIMER_LEFT_1_TM=62.232
PRIMER_RIGHT_1_TM=60.139
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=8.483659
PRIMER_LEFT_2_PENALTY=7.232188
PRIMER_RIGHT_2_PENALTY=1.251471
PRIMER_LEFT_2_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_2_SEQUENCE=TTCAACCAAGGCACAGTTTC
PRIMER_LEFT_2=749,25
PRIMER_RIGHT_2=1720,20
PRIMER_LEFT_2_TM=62.232
PRIMER_RIGHT_2_TM=58.749
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=972
PRIMER_PAIR_3_PENALTY=8.960433
PRIMER_LEFT_3_PENALTY=7.232188
PRIMER_RIGHT_3_PENALTY=1.728245
PRIMER_LEFT_3_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_3_SEQUENCE=CTTTTCAACCAAGGCACAGTT
PRIMER_LEFT_3=749,25
PRIMER_RIGHT_3=1723,21
PRIMER_LEFT_3_TM=62.232
PRIMER_RIGHT_3_TM=59.272
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=975
PRIMER_PAIR_4_PENALTY=8.976615
PRIMER_LEFT_4_PENALTY=7.232188
PRIMER_RIGHT_4_PENALTY=1.744427
PRIMER_LEFT_4_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_4_SEQUENCE=TTTCAACCAAGGCACAGTTT
PRIMER_LEFT_4=749,25
PRIMER_RIGHT_4=1721,20
PRIMER_LEFT_4_TM=62.232
PRIMER_RIGHT_4_TM=58.256
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=973
PRIMER_PAIR_5_PENALTY=8.976615
PRIMER_LEFT_5_PENALTY=7.232188
PRIMER_RIGHT_5_PENALTY=1.744427
PRIMER_LEFT_5_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_5_SEQUENCE=TTTTCAACCAAGGCACAGTT
PRIMER_LEFT_5=749,25
PRIMER_RIGHT_5=1722,20
PRIMER_LEFT_5_TM=62.232
PRIMER_RIGHT_5_TM=58.256
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=974
PRIMER_PAIR_6_PENALTY=9.306996
PRIMER_LEFT_6_PENALTY=7.232188
PRIMER_RIGHT_6_PENALTY=2.074807
PRIMER_LEFT_6_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_6_SEQUENCE=ACTTTTCAACCAAGGCACAGTT
PRIMER_LEFT_6=749,25
PRIMER_RIGHT_6=1724,22
PRIMER_LEFT_6_TM=62.232
PRIMER_RIGHT_6_TM=60.075
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=976
PRIMER_PAIR_7_PENALTY=9.376352
PRIMER_LEFT_7_PENALTY=7.232188
PRIMER_RIGHT_7_PENALTY=2.144164
PRIMER_LEFT_7_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_7_SEQUENCE=ACTTTTCAACCAAGGCACAG
PRIMER_LEFT_7=749,25
PRIMER_RIGHT_7=1724,20
PRIMER_LEFT_7_TM=62.232
PRIMER_RIGHT_7_TM=57.856
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=976
PRIMER_PAIR_8_PENALTY=9.776557
PRIMER_LEFT_8_PENALTY=7.232188
PRIMER_RIGHT_8_PENALTY=2.544368
PRIMER_LEFT_8_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_8_SEQUENCE=CTTTTCAACCAAGGCACAGTTT
PRIMER_LEFT_8=749,25
PRIMER_RIGHT_8=1723,22
PRIMER_LEFT_8_TM=62.232
PRIMER_RIGHT_8_TM=60.544
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=975
PRIMER_PAIR_9_PENALTY=9.851812
PRIMER_LEFT_9_PENALTY=7.232188
PRIMER_RIGHT_9_PENALTY=2.619623
PRIMER_LEFT_9_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_9_SEQUENCE=TTCAACCAAGGCACAGTTTCA
PRIMER_LEFT_9=749,25
PRIMER_RIGHT_9=1720,21
PRIMER_LEFT_9_TM=62.232
PRIMER_RIGHT_9_TM=61.620
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=972
PRIMER_PAIR_10_PENALTY=9.923869
PRIMER_LEFT_10_PENALTY=7.232188
PRIMER_RIGHT_10_PENALTY=2.691680
PRIMER_LEFT_10_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_10_SEQUENCE=TTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_10=749,25
PRIMER_RIGHT_10=1726,22
PRIMER_LEFT_10_TM=62.232
PRIMER_RIGHT_10_TM=59.308
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=978
PRIMER_PAIR_11_PENALTY=9.923869
PRIMER_LEFT_11_PENALTY=7.232188
PRIMER_RIGHT_11_PENALTY=2.691680
PRIMER_LEFT_11_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_11_SEQUENCE=CTTACTTTTCAACCAAGGCACA
PRIMER_LEFT_11=749,25
PRIMER_RIGHT_11=1727,22
PRIMER_LEFT_11_TM=62.232
PRIMER_RIGHT_11_TM=59.308
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=979
PRIMER_PAIR_12_PENALTY=10.283811
PRIMER_LEFT_12_PENALTY=7.232188
PRIMER_RIGHT_12_PENALTY=3.051623
PRIMER_LEFT_12_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_12_SEQUENCE=TACTTTTCAACCAAGGCACAG
PRIMER_LEFT_12=749,25
PRIMER_RIGHT_12=1725,21
PRIMER_LEFT_12_TM=62.232
PRIMER_RIGHT_12_TM=57.948
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=977
PRIMER_PAIR_13_PENALTY=10.382248
PRIMER_LEFT_13_PENALTY=7.232188
PRIMER_RIGHT_13_PENALTY=3.150060
PRIMER_LEFT_13_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_13_SEQUENCE=TACTTTTCAACCAAGGCACA
PRIMER_LEFT_13=749,25
PRIMER_RIGHT_13=1725,20
PRIMER_LEFT_13_TM=62.232
PRIMER_RIGHT_13_TM=56.850
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=977
PRIMER_PAIR_14_PENALTY=10.433304
PRIMER_LEFT_14_PENALTY=7.232188
PRIMER_RIGHT_14_PENALTY=3.201115
PRIMER_LEFT_14_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_14_SEQUENCE=CTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_14=749,25
PRIMER_RIGHT_14=1727,23
PRIMER_LEFT_14_TM=62.232
PRIMER_RIGHT_14_TM=60.201
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=979
PRIMER_PAIR_15_PENALTY=10.619517
PRIMER_LEFT_15_PENALTY=7.232188
PRIMER_RIGHT_15_PENALTY=3.387329
PRIMER_LEFT_15_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_15_SEQUENCE=TTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_15=749,25
PRIMER_RIGHT_15=1722,22
PRIMER_LEFT_15_TM=62.232
PRIMER_RIGHT_15_TM=61.387
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=974
PRIMER_PAIR_16_PENALTY=10.805592
PRIMER_LEFT_16_PENALTY=7.232188
PRIMER_RIGHT_16_PENALTY=3.573403
PRIMER_LEFT_16_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_16_SEQUENCE=TCTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_16=749,25
PRIMER_RIGHT_16=1728,22
PRIMER_LEFT_16_TM=62.232
PRIMER_RIGHT_16_TM=58.427
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=980
PRIMER_PAIR_17_PENALTY=11.602768
PRIMER_LEFT_17_PENALTY=7.232188
PRIMER_RIGHT_17_PENALTY=4.370580
PRIMER_LEFT_17_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_17_SEQUENCE=CTTACTTTTCAACCAAGGCA
PRIMER_LEFT_17=749,25
PRIMER_RIGHT_17=1727,20
PRIMER_LEFT_17_TM=62.232
PRIMER_RIGHT_17_TM=55.629
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=979
PRIMER_PAIR_18_PENALTY=11.637489
PRIMER_LEFT_18_PENALTY=7.232188
PRIMER_RIGHT_18_PENALTY=4.405301
PRIMER_LEFT_18_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_18_SEQUENCE=CTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_18=749,25
PRIMER_RIGHT_18=1727,21
PRIMER_LEFT_18_TM=62.232
PRIMER_RIGHT_18_TM=56.595
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=979
PRIMER_PAIR_19_PENALTY=11.650553
PRIMER_LEFT_19_PENALTY=7.232188
PRIMER_RIGHT_19_PENALTY=4.418365
PRIMER_LEFT_19_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_19_SEQUENCE=GTTCTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_19=749,25
PRIMER_RIGHT_19=1730,24
PRIMER_LEFT_19_TM=62.232
PRIMER_RIGHT_19_TM=60.418
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=41.667
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=982
PRIMER_PAIR_20_PENALTY=11.772755
PRIMER_LEFT_20_PENALTY=7.232188
PRIMER_RIGHT_20_PENALTY=4.540567
PRIMER_LEFT_20_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_20_SEQUENCE=TTACTTTTCAACCAAGGCAC
PRIMER_LEFT_20=749,25
PRIMER_RIGHT_20=1726,20
PRIMER_LEFT_20_TM=62.232
PRIMER_RIGHT_20_TM=55.459
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=978
PRIMER_PAIR_21_PENALTY=12.052181
PRIMER_LEFT_21_PENALTY=7.232188
PRIMER_RIGHT_21_PENALTY=4.819993
PRIMER_LEFT_21_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_21_SEQUENCE=TTTCAACCAAGGCACAGTTTCA
PRIMER_LEFT_21=749,25
PRIMER_RIGHT_21=1721,22
PRIMER_LEFT_21_TM=62.232
PRIMER_RIGHT_21_TM=62.820
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=973
PRIMER_PAIR_22_PENALTY=12.424022
PRIMER_LEFT_22_PENALTY=7.232188
PRIMER_RIGHT_22_PENALTY=5.191833
PRIMER_LEFT_22_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_22_SEQUENCE=CTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_22=749,25
PRIMER_RIGHT_22=1723,23
PRIMER_LEFT_22_TM=62.232
PRIMER_RIGHT_22_TM=62.192
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=43.478
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=975
PRIMER_PAIR_23_PENALTY=13.045001
PRIMER_LEFT_23_PENALTY=7.232188
PRIMER_RIGHT_23_PENALTY=5.812812
PRIMER_LEFT_23_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_23_SEQUENCE=TCTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_23=749,25
PRIMER_RIGHT_23=1728,24
PRIMER_LEFT_23_TM=62.232
PRIMER_RIGHT_23_TM=61.813
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=41.667
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=980
PRIMER_PAIR_24_PENALTY=14.067425
PRIMER_LEFT_24_PENALTY=7.232188
PRIMER_RIGHT_24_PENALTY=6.835237
PRIMER_LEFT_24_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_24_SEQUENCE=ACTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_24=749,25
PRIMER_RIGHT_24=1724,24
PRIMER_LEFT_24_TM=62.232
PRIMER_RIGHT_24_TM=62.835
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=41.667
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=976
PRIMER_PAIR_25_PENALTY=14.210580
PRIMER_LEFT_25_PENALTY=7.232188
PRIMER_RIGHT_25_PENALTY=6.978392
PRIMER_LEFT_25_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_25_SEQUENCE=CTTACTTTTCAACCAAGGCACAGTT
PRIMER_LEFT_25=749,25
PRIMER_RIGHT_25=1727,25
PRIMER_LEFT_25_TM=62.232
PRIMER_RIGHT_25_TM=61.978
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=979
PRIMER_PAIR_26_PENALTY=14.866289
PRIMER_LEFT_26_PENALTY=7.232188
PRIMER_RIGHT_26_PENALTY=7.634100
PRIMER_LEFT_26_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_26_SEQUENCE=CGTTCTTACTTTTCAACCAAGGCA
PRIMER_LEFT_26=749,25
PRIMER_RIGHT_26=1731,24
PRIMER_LEFT_26_TM=62.232
PRIMER_RIGHT_26_TM=63.634
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=41.667
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=983
PRIMER_PAIR_27_PENALTY=14.984772
PRIMER_LEFT_27_PENALTY=7.232188
PRIMER_RIGHT_27_PENALTY=7.752584
PRIMER_LEFT_27_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_27_SEQUENCE=TACTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_27=749,25
PRIMER_RIGHT_27=1725,25
PRIMER_LEFT_27_TM=62.232
PRIMER_RIGHT_27_TM=62.753
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=977
PRIMER_PAIR_28_PENALTY=14.984772
PRIMER_LEFT_28_PENALTY=7.232188
PRIMER_RIGHT_28_PENALTY=7.752584
PRIMER_LEFT_28_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_28_SEQUENCE=GTTCTTACTTTTCAACCAAGGCACA
PRIMER_LEFT_28=749,25
PRIMER_RIGHT_28=1730,25
PRIMER_LEFT_28_TM=62.232
PRIMER_RIGHT_28_TM=62.753
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=982
PRIMER_PAIR_29_PENALTY=15.077551
PRIMER_LEFT_29_PENALTY=7.232188
PRIMER_RIGHT_29_PENALTY=7.845362
PRIMER_LEFT_29_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_29_SEQUENCE=TTCTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_29=749,25
PRIMER_RIGHT_29=1729,25
PRIMER_LEFT_29_TM=62.232
PRIMER_RIGHT_29_TM=62.845
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=981
PRIMER_PAIR_30_PENALTY=15.811853
PRIMER_LEFT_30_PENALTY=7.232188
PRIMER_RIGHT_30_PENALTY=8.579664
PRIMER_LEFT_30_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_30_SEQUENCE=CTTTTCAACCAAGGCACAGTTTCA
PRIMER_LEFT_30=749,25
PRIMER_RIGHT_30=1723,24
PRIMER_LEFT_30_TM=62.232
PRIMER_RIGHT_30_TM=64.580
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=41.667
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.3000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=975
PRIMER_PAIR_31_PENALTY=16.425105
PRIMER_LEFT_31_PENALTY=7.232188
PRIMER_RIGHT_31_PENALTY=9.192916
PRIMER_LEFT_31_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_31_SEQUENCE=CGTTCTTACTTTTCAACCAAGGCAC
PRIMER_LEFT_31=749,25
PRIMER_RIGHT_31=1731,25
PRIMER_LEFT_31_TM=62.232
PRIMER_RIGHT_31_TM=64.193
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=44.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=983
PRIMER_PAIR_32_PENALTY=16.655433
PRIMER_LEFT_32_PENALTY=7.232188
PRIMER_RIGHT_32_PENALTY=9.423244
PRIMER_LEFT_32_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_32_SEQUENCE=GTTCTTACTTTTCAACCAAGGCACAG
PRIMER_LEFT_32=749,25
PRIMER_RIGHT_32=1730,26
PRIMER_LEFT_32_TM=62.232
PRIMER_RIGHT_32_TM=63.423
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=42.308
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=982
PRIMER_PAIR_33_PENALTY=18.544545
PRIMER_LEFT_33_PENALTY=7.232188
PRIMER_RIGHT_33_PENALTY=11.312356
PRIMER_LEFT_33_SEQUENCE=CTGCAATTAATCTCCAAATCCACTC
PRIMER_RIGHT_33_SEQUENCE=CTTACTTTTCAACCAAGGCACAGTTTC
PRIMER_LEFT_33=749,25
PRIMER_RIGHT_33=1727,27
PRIMER_LEFT_33_TM=62.232
PRIMER_RIGHT_33_TM=64.312
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=40.741
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=979
=
SEQUENCE_ID=MAGIC.329_5_9912944_9913699_edi-0_col-0_40728_8614870_TRUE
SEQUENCE_TARGET=950,855
SEQUENCE_TEMPLATE=GCNTCTAAACCCAAATCAGATGTCCAACCATTCAAGAAAATTCGTTATAGTTCTGAACATGGTTGTTTGACCATGAGTTTTGATTCCCCTTCTTCATGTCCTTCAACCTCATTTTTANGTGATTTATCACNAAATCTAGTTCGTGGCAATGCTCATATNCCANAGTTTTATACAAGGGATCTCATTTCAACCATTNGAAGTCGTGACCACTACTCGGTAGATCTTATTCCAAAGTTTGGNGAAAACACTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCTCTCTGGCTCTCTGTCGCACAACTTCCTTCGCACGGCAGCCCTCCGCCGCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4717000_4717213_ct-1_mt-0_1386_3624_TRUE
SEQUENCE_TARGET=950,313
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_8613637_8614042_ct-1_wil-2_765760_315701_TRUE
SEQUENCE_TARGET=950,505
SEQUENCE_TEMPLATE=GGTGTGTTTTATATAAAATCAAATAAATGTCATTTTTATAATTTATGTTTGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTATGCAAAATTGTCACGAACTAAAACATAATAAGCCTAACATATTAGTTCTNAAAACTTCAACATCTATAGCTATCATNCTACGACATGGTCNNTANATNGACCANATCTACTTGGTACGACAGTGTNNGACTTTCGTCTNAATTTATTGGCAAATTTTTCGTTAGANTATATATCCGACAAGAGATTTTGGGAGTGCTTTTTGCGAAGTGACTNAAGATGGGATCAAGACACTTTNTGANTTTCAGACANGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9444526_9444577_no-0_wil-2_7659_1202_TRUE
SEQUENCE_TARGET=950,151
SEQUENCE_TEMPLATE=AAAAATNAATCAANACATGTATAAACAAANTGNNAAAAAANCACANAAAAAATATGGTGCTTTTCTGAGGNNNNNNNNNTTTTTTNTTTTCGTCTGATCTTATATTNNAAATTTGGNTTTTATATTCAANNNTTTNNTATATTTTGTGGTCATANATTGTCCAAGATAACAATTCGTGACCAAANNAAATATATGTGATTATTTTANAGANAACAANTAATTACNNAAAAANAAATNATATNTTCNAAATAAAATGTAAGANAAAANAAGAAGATTATATATGTNAGAACTTTNTGAGCTAAATAAAATGAAGNAAAGTCATNGNTTAGNCGCGTAGNAAATCATTCAGAATTCAGAAATATGGCTCNTCGCTAATATTTNTCTTTTANNNNNNNNNNTNTTTCNACCTAATTTGAGAGCANTGNATATCGATAGGTTTCACATTAGCTTNNNNNNNNNACATANATGATTGTGCNAAAAAACAGAAACGATNGTTNANTANTNNTCTAAGAAAGTGACATTTNAAAAAGGCTCATTTCAATATAGAGNTGCCTTCTTCATGACATGACCTTNATATGATAAATTCCCTAATGTAATGAATCAATAAAACGAACTTATANAACGGTAGNAATTACGTACGTGAGGATTTATATCATCTANTAATTAATTAATTGACAACAAAAACAGAAGGGGTAGTGTTCACGTGCATGTCACGCGGGNAATGGTGTGGACACACGAGCCTCGTACGGTGTAAAGAAGGAGAACAGAGAGAGTCGCACGAGNCNCACGTGGGATGTTGACTTGTCGATTAGCTTAAGGGGTATGTCCATATNCTAATGATTAAGTACAGTCGAATGAAAANATGCCACNANCCCTAGATCTANATGTTTATTGNGGNNNNTTTTNTANTCAGTCACATCCNNATGATTAATATTGGTCCCTGATATTCATCTTATATATTTCACAANTATAACAATCACCCAAACATTAGTTTTTCTTANATTGTTTTACNGTGAAATGTTTCACTGTTTCCTNTATGANTAATTAACTANTGGANNGGAATTTATTCATTTATCATATNCANATAATTCATCTTTATTTTCATTTGTGNATATANNANATATCCTTTNNCTTTCTCATGCTTACCATTTAGNCTNTTTNTCTTCCTCAACCTTCTTNNNNNNNNNNNNNNNCCTTAAAAACNGATGAAANTANTCTTTNCTCCNATGAATTTTNCCAATCACTTTACAAATTATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGTTGAGTGNAATCCTTTCACAAGNTTTTCTTAAGAAANTGAAAAACAATCATANATGTACAATACTAGAGATATATNATGAGTCATGANTCATATTAATCGCGGTTTTTGTAATATCATAAAGCTTTCTACTTTAGGCTTGNCAGTGATTNAATACAGTAACATATCACATAAATATGAGAAATGGAGGCTCTTTAATTAGGCCTTTACAATATACATATATATTAGAAATAGAAAGAAAAGNAAAAANTCGNCCGACAACAAGATTGGTGAAGAAACGCNATAATTGAACCATTAAGTACAGTCTAATACCCGCAGAAAATATNACACCACCTACTCTTAGTGGCAATGCTGTACTTTAACATNTTGTCGAGACAAGGAGNCCAGGTTNCGTGAAATCATGNAGATGANGNNGCATAAACAATCACCAAAGTTCACCCTATGAATCCAANCCCTTCTAGCTTAGTGGCTATGATCATAGCCGACATCTCATANAGTCANANTCCATTTCGGAGTAAATNATGCGNTGAACAANANTNAGCTTGTTCNCATTNCAANATANCAAATTGGATACTATATNNNAAAAANNNNNGANTGTNANNNNTTNGATCAAATCTAACTNTTAGAGANNNATNATANAANAAACATACATGATTTNAAGTAGTTTACTATAATTTNNATANANTTAGAATTTGGAAANAGNGAACGATTATAGATTNTGNTAGTAGANTTTGGAAANAGAAATGCTNTNAAANAACNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.465248
PRIMER_LEFT_0_PENALTY=1.006255
PRIMER_RIGHT_0_PENALTY=0.458994
PRIMER_LEFT_0_SEQUENCE=TGACAACAAAAACAGAAGGGG
PRIMER_RIGHT_0_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_0=672,21
PRIMER_RIGHT_0=1563,20
PRIMER_LEFT_0_TM=59.994
PRIMER_RIGHT_0_TM=59.541
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=892
PRIMER_PAIR_1_PENALTY=1.827444
PRIMER_LEFT_1_PENALTY=1.368450
PRIMER_RIGHT_1_PENALTY=0.458994
PRIMER_LEFT_1_SEQUENCE=TGGGATGTTGACTTGTCGATT
PRIMER_RIGHT_1_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_1=789,21
PRIMER_RIGHT_1=1563,20
PRIMER_LEFT_1_TM=60.368
PRIMER_RIGHT_1_TM=59.541
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=775
PRIMER_PAIR_2_PENALTY=2.066998
PRIMER_LEFT_2_PENALTY=1.608005
PRIMER_RIGHT_2_PENALTY=0.458994
PRIMER_LEFT_2_SEQUENCE=GAGCCTCGTACGGTGTAAAGA
PRIMER_RIGHT_2_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_2=736,21
PRIMER_RIGHT_2=1563,20
PRIMER_LEFT_2_TM=59.392
PRIMER_RIGHT_2_TM=59.541
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=828
PRIMER_PAIR_3_PENALTY=2.118852
PRIMER_LEFT_3_PENALTY=1.659858
PRIMER_RIGHT_3_PENALTY=0.458994
PRIMER_LEFT_3_SEQUENCE=AGGAGAACAGAGAGAGTCGCA
PRIMER_RIGHT_3_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_3=757,21
PRIMER_RIGHT_3=1563,20
PRIMER_LEFT_3_TM=59.340
PRIMER_RIGHT_3_TM=59.541
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=807
PRIMER_PAIR_4_PENALTY=2.213269
PRIMER_LEFT_4_PENALTY=1.754275
PRIMER_RIGHT_4_PENALTY=0.458994
PRIMER_LEFT_4_SEQUENCE=GGAGAACAGAGAGAGTCGCA
PRIMER_RIGHT_4_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_4=758,20
PRIMER_RIGHT_4=1563,20
PRIMER_LEFT_4_TM=58.246
PRIMER_RIGHT_4_TM=59.541
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=806
PRIMER_PAIR_5_PENALTY=2.297795
PRIMER_LEFT_5_PENALTY=1.006255
PRIMER_RIGHT_5_PENALTY=1.291540
PRIMER_LEFT_5_SEQUENCE=TGACAACAAAAACAGAAGGGG
PRIMER_RIGHT_5_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_5=672,21
PRIMER_RIGHT_5=1494,21
PRIMER_LEFT_5_TM=59.994
PRIMER_RIGHT_5_TM=59.708
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=10.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=823
PRIMER_PAIR_6_PENALTY=2.542162
PRIMER_LEFT_6_PENALTY=2.083168
PRIMER_RIGHT_6_PENALTY=0.458994
PRIMER_LEFT_6_SEQUENCE=GCCTCGTACGGTGTAAAGAAG
PRIMER_RIGHT_6_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_6=738,21
PRIMER_RIGHT_6=1563,20
PRIMER_LEFT_6_TM=58.917
PRIMER_RIGHT_6_TM=59.541
PRIMER_LEFT_6_GC_PERCENT=52.381
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=826
PRIMER_PAIR_7_PENALTY=2.542162
PRIMER_LEFT_7_PENALTY=2.083168
PRIMER_RIGHT_7_PENALTY=0.458994
PRIMER_LEFT_7_SEQUENCE=AGCCTCGTACGGTGTAAAGAA
PRIMER_RIGHT_7_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_7=737,21
PRIMER_RIGHT_7=1563,20
PRIMER_LEFT_7_TM=58.917
PRIMER_RIGHT_7_TM=59.541
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=827
PRIMER_PAIR_8_PENALTY=2.553169
PRIMER_LEFT_8_PENALTY=2.094176
PRIMER_RIGHT_8_PENALTY=0.458994
PRIMER_LEFT_8_SEQUENCE=GCCTCGTACGGTGTAAAGAA
PRIMER_RIGHT_8_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_8=738,20
PRIMER_RIGHT_8=1563,20
PRIMER_LEFT_8_TM=57.906
PRIMER_RIGHT_8_TM=59.541
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=826
PRIMER_PAIR_9_PENALTY=2.618673
PRIMER_LEFT_9_PENALTY=2.159680
PRIMER_RIGHT_9_PENALTY=0.458994
PRIMER_LEFT_9_SEQUENCE=AGCCTCGTACGGTGTAAAGAAG
PRIMER_RIGHT_9_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_9=737,22
PRIMER_RIGHT_9=1563,20
PRIMER_LEFT_9_TM=59.840
PRIMER_RIGHT_9_TM=59.541
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=827
PRIMER_PAIR_10_PENALTY=2.659990
PRIMER_LEFT_10_PENALTY=1.368450
PRIMER_RIGHT_10_PENALTY=1.291540
PRIMER_LEFT_10_SEQUENCE=TGGGATGTTGACTTGTCGATT
PRIMER_RIGHT_10_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_10=789,21
PRIMER_RIGHT_10=1494,21
PRIMER_LEFT_10_TM=60.368
PRIMER_RIGHT_10_TM=59.708
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.6000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=706
PRIMER_PAIR_11_PENALTY=2.682774
PRIMER_LEFT_11_PENALTY=2.223781
PRIMER_RIGHT_11_PENALTY=0.458994
PRIMER_LEFT_11_SEQUENCE=CCTCGTACGGTGTAAAGAAGG
PRIMER_RIGHT_11_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_11=739,21
PRIMER_RIGHT_11=1563,20
PRIMER_LEFT_11_TM=58.776
PRIMER_RIGHT_11_TM=59.541
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=825
PRIMER_PAIR_12_PENALTY=2.899545
PRIMER_LEFT_12_PENALTY=1.608005
PRIMER_RIGHT_12_PENALTY=1.291540
PRIMER_LEFT_12_SEQUENCE=GAGCCTCGTACGGTGTAAAGA
PRIMER_RIGHT_12_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_12=736,21
PRIMER_RIGHT_12=1494,21
PRIMER_LEFT_12_TM=59.392
PRIMER_RIGHT_12_TM=59.708
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=759
PRIMER_PAIR_13_PENALTY=2.946760
PRIMER_LEFT_13_PENALTY=2.487767
PRIMER_RIGHT_13_PENALTY=0.458994
PRIMER_LEFT_13_SEQUENCE=AGCCTCGTACGGTGTAAAGA
PRIMER_RIGHT_13_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_13=737,20
PRIMER_RIGHT_13=1563,20
PRIMER_LEFT_13_TM=57.512
PRIMER_RIGHT_13_TM=59.541
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=827
PRIMER_PAIR_14_PENALTY=2.946760
PRIMER_LEFT_14_PENALTY=2.487767
PRIMER_RIGHT_14_PENALTY=0.458994
PRIMER_LEFT_14_SEQUENCE=GAGCCTCGTACGGTGTAAAG
PRIMER_RIGHT_14_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_14=736,20
PRIMER_RIGHT_14=1563,20
PRIMER_LEFT_14_TM=57.512
PRIMER_RIGHT_14_TM=59.541
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.3000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=828
PRIMER_PAIR_15_PENALTY=2.951399
PRIMER_LEFT_15_PENALTY=1.659858
PRIMER_RIGHT_15_PENALTY=1.291540
PRIMER_LEFT_15_SEQUENCE=AGGAGAACAGAGAGAGTCGCA
PRIMER_RIGHT_15_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_15=757,21
PRIMER_RIGHT_15=1494,21
PRIMER_LEFT_15_TM=59.340
PRIMER_RIGHT_15_TM=59.708
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=10.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=738
PRIMER_PAIR_16_PENALTY=2.983961
PRIMER_LEFT_16_PENALTY=2.524968
PRIMER_RIGHT_16_PENALTY=0.458994
PRIMER_LEFT_16_SEQUENCE=CCTCGTACGGTGTAAAGAAGGA
PRIMER_RIGHT_16_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_16=739,22
PRIMER_RIGHT_16=1563,20
PRIMER_LEFT_16_TM=60.525
PRIMER_RIGHT_16_TM=59.541
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=825
PRIMER_PAIR_17_PENALTY=3.041008
PRIMER_LEFT_17_PENALTY=2.582014
PRIMER_RIGHT_17_PENALTY=0.458994
PRIMER_LEFT_17_SEQUENCE=GGGATGTTGACTTGTCGATT
PRIMER_RIGHT_17_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_17=790,20
PRIMER_RIGHT_17=1563,20
PRIMER_LEFT_17_TM=57.418
PRIMER_RIGHT_17_TM=59.541
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.6000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=774
PRIMER_PAIR_18_PENALTY=3.045816
PRIMER_LEFT_18_PENALTY=1.754275
PRIMER_RIGHT_18_PENALTY=1.291540
PRIMER_LEFT_18_SEQUENCE=GGAGAACAGAGAGAGTCGCA
PRIMER_RIGHT_18_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_18=758,20
PRIMER_RIGHT_18=1494,21
PRIMER_LEFT_18_TM=58.246
PRIMER_RIGHT_18_TM=59.708
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=10.2000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=737
PRIMER_PAIR_19_PENALTY=3.120193
PRIMER_LEFT_19_PENALTY=2.661199
PRIMER_RIGHT_19_PENALTY=0.458994
PRIMER_LEFT_19_SEQUENCE=GAGCCTCGTACGGTGTAAAGAA
PRIMER_RIGHT_19_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_19=736,22
PRIMER_RIGHT_19=1563,20
PRIMER_LEFT_19_TM=60.661
PRIMER_RIGHT_19_TM=59.541
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=828
PRIMER_PAIR_20_PENALTY=3.160118
PRIMER_LEFT_20_PENALTY=2.701125
PRIMER_RIGHT_20_PENALTY=0.458994
PRIMER_LEFT_20_SEQUENCE=AAGGAGAACAGAGAGAGTCGCA
PRIMER_RIGHT_20_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_20=756,22
PRIMER_RIGHT_20=1563,20
PRIMER_LEFT_20_TM=60.701
PRIMER_RIGHT_20_TM=59.541
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=10.2000
PRIMER_RIGHT_20_END_STABILITY=9.6000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=808
PRIMER_PAIR_21_PENALTY=3.241561
PRIMER_LEFT_21_PENALTY=2.782568
PRIMER_RIGHT_21_PENALTY=0.458994
PRIMER_LEFT_21_SEQUENCE=TGACAACAAAAACAGAAGGGGT
PRIMER_RIGHT_21_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_21=672,22
PRIMER_RIGHT_21=1563,20
PRIMER_LEFT_21_TM=60.783
PRIMER_RIGHT_21_TM=59.541
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=10.6000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=8.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=892
PRIMER_PAIR_22_PENALTY=3.311670
PRIMER_LEFT_22_PENALTY=2.852676
PRIMER_RIGHT_22_PENALTY=0.458994
PRIMER_LEFT_22_SEQUENCE=GACAACAAAAACAGAAGGGG
PRIMER_RIGHT_22_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_22=673,20
PRIMER_RIGHT_22=1563,20
PRIMER_LEFT_22_TM=57.147
PRIMER_RIGHT_22_TM=59.541
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=1.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=10.9000
PRIMER_RIGHT_22_END_STABILITY=9.6000
PRIMER_PAIR_22_COMPL_ANY=8.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=891
PRIMER_PAIR_23_PENALTY=3.374709
PRIMER_LEFT_23_PENALTY=2.083168
PRIMER_RIGHT_23_PENALTY=1.291540
PRIMER_LEFT_23_SEQUENCE=GCCTCGTACGGTGTAAAGAAG
PRIMER_RIGHT_23_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_23=738,21
PRIMER_RIGHT_23=1494,21
PRIMER_LEFT_23_TM=58.917
PRIMER_RIGHT_23_TM=59.708
PRIMER_LEFT_23_GC_PERCENT=52.381
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=757
PRIMER_PAIR_24_PENALTY=3.374709
PRIMER_LEFT_24_PENALTY=2.083168
PRIMER_RIGHT_24_PENALTY=1.291540
PRIMER_LEFT_24_SEQUENCE=AGCCTCGTACGGTGTAAAGAA
PRIMER_RIGHT_24_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_24=737,21
PRIMER_RIGHT_24=1494,21
PRIMER_LEFT_24_TM=58.917
PRIMER_RIGHT_24_TM=59.708
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=758
PRIMER_PAIR_25_PENALTY=3.385716
PRIMER_LEFT_25_PENALTY=2.094176
PRIMER_RIGHT_25_PENALTY=1.291540
PRIMER_LEFT_25_SEQUENCE=GCCTCGTACGGTGTAAAGAA
PRIMER_RIGHT_25_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_25=738,20
PRIMER_RIGHT_25=1494,21
PRIMER_LEFT_25_TM=57.906
PRIMER_RIGHT_25_TM=59.708
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=757
PRIMER_PAIR_26_PENALTY=3.389996
PRIMER_LEFT_26_PENALTY=2.931002
PRIMER_RIGHT_26_PENALTY=0.458994
PRIMER_LEFT_26_SEQUENCE=GACAACAAAAACAGAAGGGGT
PRIMER_RIGHT_26_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_26=673,21
PRIMER_RIGHT_26=1563,20
PRIMER_LEFT_26_TM=58.069
PRIMER_RIGHT_26_TM=59.541
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=10.6000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=8.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=891
PRIMER_PAIR_27_PENALTY=3.419725
PRIMER_LEFT_27_PENALTY=2.960732
PRIMER_RIGHT_27_PENALTY=0.458994
PRIMER_LEFT_27_SEQUENCE=CTTGTCGATTAGCTTAAGGGG
PRIMER_RIGHT_27_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_27=800,21
PRIMER_RIGHT_27=1563,20
PRIMER_LEFT_27_TM=58.039
PRIMER_RIGHT_27_TM=59.541
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=10.9000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=764
PRIMER_PAIR_28_PENALTY=3.436192
PRIMER_LEFT_28_PENALTY=2.977199
PRIMER_RIGHT_28_PENALTY=0.458994
PRIMER_LEFT_28_SEQUENCE=TTGTCGATTAGCTTAAGGGG
PRIMER_RIGHT_28_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_28=801,20
PRIMER_RIGHT_28=1563,20
PRIMER_LEFT_28_TM=57.023
PRIMER_RIGHT_28_TM=59.541
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=10.9000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=763
PRIMER_PAIR_29_PENALTY=3.451220
PRIMER_LEFT_29_PENALTY=2.159680
PRIMER_RIGHT_29_PENALTY=1.291540
PRIMER_LEFT_29_SEQUENCE=AGCCTCGTACGGTGTAAAGAAG
PRIMER_RIGHT_29_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_29=737,22
PRIMER_RIGHT_29=1494,21
PRIMER_LEFT_29_TM=59.840
PRIMER_RIGHT_29_TM=59.708
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=758
PRIMER_PAIR_30_PENALTY=3.498808
PRIMER_LEFT_30_PENALTY=1.368450
PRIMER_RIGHT_30_PENALTY=2.130358
PRIMER_LEFT_30_SEQUENCE=TGGGATGTTGACTTGTCGATT
PRIMER_RIGHT_30_SEQUENCE=GGGTGAACTTTGGTGATTGTTT
PRIMER_LEFT_30=789,21
PRIMER_RIGHT_30=1729,22
PRIMER_LEFT_30_TM=60.368
PRIMER_RIGHT_30_TM=60.130
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.6000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=941
PRIMER_PAIR_31_PENALTY=3.515321
PRIMER_LEFT_31_PENALTY=2.223781
PRIMER_RIGHT_31_PENALTY=1.291540
PRIMER_LEFT_31_SEQUENCE=CCTCGTACGGTGTAAAGAAGG
PRIMER_RIGHT_31_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_31=739,21
PRIMER_RIGHT_31=1494,21
PRIMER_LEFT_31_TM=58.776
PRIMER_RIGHT_31_TM=59.708
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=756
PRIMER_PAIR_32_PENALTY=3.555419
PRIMER_LEFT_32_PENALTY=3.096426
PRIMER_RIGHT_32_PENALTY=0.458994
PRIMER_LEFT_32_SEQUENCE=TCGTACGGTGTAAAGAAGGAGA
PRIMER_RIGHT_32_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_32=741,22
PRIMER_RIGHT_32=1563,20
PRIMER_LEFT_32_TM=58.904
PRIMER_RIGHT_32_TM=59.541
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=823
PRIMER_PAIR_33_PENALTY=3.558004
PRIMER_LEFT_33_PENALTY=3.099010
PRIMER_RIGHT_33_PENALTY=0.458994
PRIMER_LEFT_33_SEQUENCE=TTGTCGATTAGCTTAAGGGGT
PRIMER_RIGHT_33_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_33=801,21
PRIMER_RIGHT_33=1563,20
PRIMER_LEFT_33_TM=57.901
PRIMER_RIGHT_33_TM=59.541
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=10.6000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=763
PRIMER_PAIR_34_PENALTY=3.561978
PRIMER_LEFT_34_PENALTY=1.006255
PRIMER_RIGHT_34_PENALTY=2.555723
PRIMER_LEFT_34_SEQUENCE=TGACAACAAAAACAGAAGGGG
PRIMER_RIGHT_34_SEQUENCE=AGGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_34=672,21
PRIMER_RIGHT_34=1495,22
PRIMER_LEFT_34_TM=59.994
PRIMER_RIGHT_34_TM=60.556
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=10.9000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=824
PRIMER_PAIR_35_PENALTY=3.565195
PRIMER_LEFT_35_PENALTY=1.368450
PRIMER_RIGHT_35_PENALTY=2.196745
PRIMER_LEFT_35_SEQUENCE=TGGGATGTTGACTTGTCGATT
PRIMER_RIGHT_35_SEQUENCE=GGGTGAACTTTGGTGATTGTT
PRIMER_LEFT_35=789,21
PRIMER_RIGHT_35=1729,21
PRIMER_LEFT_35_TM=60.368
PRIMER_RIGHT_35_TM=58.803
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.6000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=941
PRIMER_PAIR_36_PENALTY=3.595421
PRIMER_LEFT_36_PENALTY=1.368450
PRIMER_RIGHT_36_PENALTY=2.226971
PRIMER_LEFT_36_SEQUENCE=TGGGATGTTGACTTGTCGATT
PRIMER_RIGHT_36_SEQUENCE=AGGGTGAACTTTGGTGATTGTT
PRIMER_LEFT_36=789,21
PRIMER_RIGHT_36=1730,22
PRIMER_LEFT_36_TM=60.368
PRIMER_RIGHT_36_TM=59.773
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.6000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=942
PRIMER_PAIR_37_PENALTY=3.614308
PRIMER_LEFT_37_PENALTY=3.155314
PRIMER_RIGHT_37_PENALTY=0.458994
PRIMER_LEFT_37_SEQUENCE=CTTGTCGATTAGCTTAAGGGGT
PRIMER_RIGHT_37_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_37=800,22
PRIMER_RIGHT_37=1563,20
PRIMER_LEFT_37_TM=58.845
PRIMER_RIGHT_37_TM=59.541
PRIMER_LEFT_37_GC_PERCENT=45.455
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.6000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=764
PRIMER_PAIR_38_PENALTY=3.614308
PRIMER_LEFT_38_PENALTY=3.155314
PRIMER_RIGHT_38_PENALTY=0.458994
PRIMER_LEFT_38_SEQUENCE=ACTTGTCGATTAGCTTAAGGGG
PRIMER_RIGHT_38_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_38=799,22
PRIMER_RIGHT_38=1563,20
PRIMER_LEFT_38_TM=58.845
PRIMER_RIGHT_38_TM=59.541
PRIMER_LEFT_38_GC_PERCENT=45.455
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=10.9000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=765
PRIMER_PAIR_39_PENALTY=3.627671
PRIMER_LEFT_39_PENALTY=3.168677
PRIMER_RIGHT_39_PENALTY=0.458994
PRIMER_LEFT_39_SEQUENCE=TCGTACGGTGTAAAGAAGGAGAA
PRIMER_RIGHT_39_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_39=741,23
PRIMER_RIGHT_39=1563,20
PRIMER_LEFT_39_TM=60.169
PRIMER_RIGHT_39_TM=59.541
PRIMER_LEFT_39_GC_PERCENT=43.478
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=823
PRIMER_PAIR_40_PENALTY=3.631536
PRIMER_LEFT_40_PENALTY=3.172542
PRIMER_RIGHT_40_PENALTY=0.458994
PRIMER_LEFT_40_SEQUENCE=GTGGGATGTTGACTTGTCGATT
PRIMER_RIGHT_40_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_40=788,22
PRIMER_RIGHT_40=1563,20
PRIMER_LEFT_40_TM=61.173
PRIMER_RIGHT_40_TM=59.541
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.6000
PRIMER_RIGHT_40_END_STABILITY=9.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=776
PRIMER_PAIR_41_PENALTY=3.631934
PRIMER_LEFT_41_PENALTY=3.172940
PRIMER_RIGHT_41_PENALTY=0.458994
PRIMER_LEFT_41_SEQUENCE=CTCGTACGGTGTAAAGAAGGAGA
PRIMER_RIGHT_41_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_41=740,23
PRIMER_RIGHT_41=1563,20
PRIMER_LEFT_41_TM=59.827
PRIMER_RIGHT_41_TM=59.541
PRIMER_LEFT_41_GC_PERCENT=47.826
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=824
PRIMER_PAIR_42_PENALTY=3.664627
PRIMER_LEFT_42_PENALTY=1.368450
PRIMER_RIGHT_42_PENALTY=2.296177
PRIMER_LEFT_42_SEQUENCE=TGGGATGTTGACTTGTCGATT
PRIMER_RIGHT_42_SEQUENCE=GGATTCATAGGGTGAACTTTGG
PRIMER_LEFT_42=789,21
PRIMER_RIGHT_42=1738,22
PRIMER_LEFT_42_TM=60.368
PRIMER_RIGHT_42_TM=59.704
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.6000
PRIMER_RIGHT_42_END_STABILITY=8.8000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=950
PRIMER_PAIR_43_PENALTY=3.701473
PRIMER_LEFT_43_PENALTY=1.006255
PRIMER_RIGHT_43_PENALTY=2.695219
PRIMER_LEFT_43_SEQUENCE=TGACAACAAAAACAGAAGGGG
PRIMER_RIGHT_43_SEQUENCE=TTGCCACTAAGAGTAGGTGGTG
PRIMER_LEFT_43=672,21
PRIMER_RIGHT_43=1638,22
PRIMER_LEFT_43_TM=59.994
PRIMER_RIGHT_43_TM=59.305
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.9000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=967
PRIMER_PAIR_44_PENALTY=3.708408
PRIMER_LEFT_44_PENALTY=3.249414
PRIMER_RIGHT_44_PENALTY=0.458994
PRIMER_LEFT_44_SEQUENCE=TTGACAACAAAAACAGAAGGGG
PRIMER_RIGHT_44_SEQUENCE=TTCACCAATCTTGTTGTCGG
PRIMER_LEFT_44=671,22
PRIMER_RIGHT_44=1563,20
PRIMER_LEFT_44_TM=61.249
PRIMER_RIGHT_44_TM=59.541
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=10.9000
PRIMER_RIGHT_44_END_STABILITY=9.6000
PRIMER_PAIR_44_COMPL_ANY=8.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=893
PRIMER_PAIR_45_PENALTY=3.738362
PRIMER_LEFT_45_PENALTY=1.608005
PRIMER_RIGHT_45_PENALTY=2.130358
PRIMER_LEFT_45_SEQUENCE=GAGCCTCGTACGGTGTAAAGA
PRIMER_RIGHT_45_SEQUENCE=GGGTGAACTTTGGTGATTGTTT
PRIMER_LEFT_45=736,21
PRIMER_RIGHT_45=1729,22
PRIMER_LEFT_45_TM=59.392
PRIMER_RIGHT_45_TM=60.130
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=994
PRIMER_PAIR_46_PENALTY=3.779307
PRIMER_LEFT_46_PENALTY=2.487767
PRIMER_RIGHT_46_PENALTY=1.291540
PRIMER_LEFT_46_SEQUENCE=AGCCTCGTACGGTGTAAAGA
PRIMER_RIGHT_46_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_46=737,20
PRIMER_RIGHT_46=1494,21
PRIMER_LEFT_46_TM=57.512
PRIMER_RIGHT_46_TM=59.708
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=758
PRIMER_PAIR_47_PENALTY=3.779307
PRIMER_LEFT_47_PENALTY=2.487767
PRIMER_RIGHT_47_PENALTY=1.291540
PRIMER_LEFT_47_SEQUENCE=GAGCCTCGTACGGTGTAAAG
PRIMER_RIGHT_47_SEQUENCE=GGCCTAATTAAAGAGCCTCCA
PRIMER_LEFT_47=736,20
PRIMER_RIGHT_47=1494,21
PRIMER_LEFT_47_TM=57.512
PRIMER_RIGHT_47_TM=59.708
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.3000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=759
PRIMER_PAIR_48_PENALTY=3.790216
PRIMER_LEFT_48_PENALTY=1.659858
PRIMER_RIGHT_48_PENALTY=2.130358
PRIMER_LEFT_48_SEQUENCE=AGGAGAACAGAGAGAGTCGCA
PRIMER_RIGHT_48_SEQUENCE=GGGTGAACTTTGGTGATTGTTT
PRIMER_LEFT_48=757,21
PRIMER_RIGHT_48=1729,22
PRIMER_LEFT_48_TM=59.340
PRIMER_RIGHT_48_TM=60.130
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=10.2000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=973
PRIMER_PAIR_49_PENALTY=3.804750
PRIMER_LEFT_49_PENALTY=1.608005
PRIMER_RIGHT_49_PENALTY=2.196745
PRIMER_LEFT_49_SEQUENCE=GAGCCTCGTACGGTGTAAAGA
PRIMER_RIGHT_49_SEQUENCE=GGGTGAACTTTGGTGATTGTT
PRIMER_LEFT_49=736,21
PRIMER_RIGHT_49=1729,21
PRIMER_LEFT_49_TM=59.392
PRIMER_RIGHT_49_TM=58.803
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=994
=
SEQUENCE_ID=MAGIC.287_4_1632626_1633050_oy-0_edi-0_3108_18025_TRUE
SEQUENCE_TARGET=950,524
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_20756920_20757144_sf-2_po-0_909410_1118964_TRUE
SEQUENCE_TARGET=950,324
SEQUENCE_TEMPLATE=TCAATGTTGTGTGCATATGGACCCGGCCCNTATAATGAGAGCTGGTNTTNTGAANTTGAACGTTGAGTATGTCTGACAGTCTGAGAGGTAGACAAGAGACAGAAAATGAAGNTATATGATGNTTTGCTTCAAATCTTCTTTTATGTTTTNAATCTTTATTTGGCTTTATCTNTTAATGTGTTTGGATTTTTCTAGAATCTGAGTTCATTTGTATTCCGATCCAATTGAAACATGTGTGAGGTTTATTCCCCCGATCTCAACATCATTTATTTGACAATACGAAGGTCAAACACCATAATAAACAGAGCGAGAACNACAATAGTAAAAACAGAGCATATGAGTGAAGGAAAATAAATAATTTCAACTAAGGTGTAGTATTAATAAATNTANGTATGCTNTCATATCAGCAAGTGGACGTGCCGGAGTGGTTATCGGGCATGACTAGAAATCATGTGGGCTTTGCCNGCGCAGGTTCGAATCCTGCCGTTCACGCTTTTTCTTTTTCAATTTTAGACAATTTCTTTCACTTTTAGATGAAATNATTTAGACGTCTCCGCATNGCCGAGAGTCTGAAAGGAACGTTGAATAATNTGCTAATGGTAAATTTGGAAAGAAAAATCNTAACGNTTTTAAAAGAAAACAATTGTATTCATAACATTATGCAAATAAACTACTGTAACCATCTACNACTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGANATNCNCTNAAATTTNNNNNCTGTNAGAGATGACAAATAACAAAACAAAAANTTTTATTAGGTCTTTGAGCTTAAGCCAACACTCCTACAACCTNTGCTACTTTTTGCACNGTTTNTCTCAAAAGAAAAAACAAANTAATCATACAANAAAANAANGCATACAAANAAATCTACAGTCAAGAGANNNNNNATATATATATATATATGTATACAAATATTAATNTTTTAAAAAATAGTAAGGGAAGTAAAATTACGAAAAACATAATAACTCATGCTTCTTTCTTTTATTTTTTTTGTTTAGGCCTTTTAACTCTGTGTGTCCTTTAGAAATTCTCTTTGTGGAAGGAGAATCTGGTGCTTGTTTTGTGGGTGAAATCTAAAGCTAGATGCCTTAGCTCCTTTGTCAATGCTGGTGCTCCACAGTAAAACACTCCTGCACATACAAAATCAAGATTCTTTAATCCAATCACTTTTCATTTCTATATAACCGCGAACATAGTAGAGAAGAGAGATGTGAGCTTACCAACTTTGGTGTTAGGATGATCCATAGCTATNCGNTTGTAAACATTTCTCCAATTAGGTTTAGCGAAATGGGACATAACTCTTGTTCCAGAGACAATGTCGACGCCGTTCTTTGCATGGTTTAGTGATTGAAGCATATGTATAAGTGCGGAACGAGCGTCACCTTCTTCATAGACACTTGTACAATAGTTATGCATTTCGATGACGCGGTTGGCATCTCGTTCCGCGACTTCGTTCATTATGTTCTTGAACCAATCGAAAGAGCCTTGCTCACGCGTAACCCAATAGAAGTAAGCTCTACGGGTCCTGAAACTCTCTTTCTTACTTCGTTGTGGTTCGCTTGTTCCATTCTCCATTCGGTTTAGTTGGGCTTGTTCCTTGGCCTTGATGTTATTAACAATGTCTTTGACGATACTGATCATTGGTGTGGCTCCAATCCCGAGACCAACTAGTAGAACCAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.762262
PRIMER_LEFT_0_PENALTY=0.620602
PRIMER_RIGHT_0_PENALTY=3.141660
PRIMER_LEFT_0_SEQUENCE=CCGTTCACGCTTTTTCTTTT
PRIMER_RIGHT_0_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_0=483,20
PRIMER_RIGHT_0=1334,21
PRIMER_LEFT_0_TM=59.379
PRIMER_RIGHT_0_TM=57.858
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=852
PRIMER_PAIR_1_PENALTY=3.855299
PRIMER_LEFT_1_PENALTY=0.620602
PRIMER_RIGHT_1_PENALTY=3.234696
PRIMER_LEFT_1_SEQUENCE=CCGTTCACGCTTTTTCTTTT
PRIMER_RIGHT_1_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_1=483,20
PRIMER_RIGHT_1=1333,20
PRIMER_LEFT_1_TM=59.379
PRIMER_RIGHT_1_TM=56.765
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=851
PRIMER_PAIR_2_PENALTY=4.123921
PRIMER_LEFT_2_PENALTY=0.982262
PRIMER_RIGHT_2_PENALTY=3.141660
PRIMER_LEFT_2_SEQUENCE=GCCGAGAGTCTGAAAGGAAC
PRIMER_RIGHT_2_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_2=560,20
PRIMER_RIGHT_2=1334,21
PRIMER_LEFT_2_TM=59.018
PRIMER_RIGHT_2_TM=57.858
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=775
PRIMER_PAIR_3_PENALTY=4.216958
PRIMER_LEFT_3_PENALTY=0.982262
PRIMER_RIGHT_3_PENALTY=3.234696
PRIMER_LEFT_3_SEQUENCE=GCCGAGAGTCTGAAAGGAAC
PRIMER_RIGHT_3_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_3=560,20
PRIMER_RIGHT_3=1333,20
PRIMER_LEFT_3_TM=59.018
PRIMER_RIGHT_3_TM=56.765
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=774
PRIMER_PAIR_4_PENALTY=4.269430
PRIMER_LEFT_4_PENALTY=0.620602
PRIMER_RIGHT_4_PENALTY=3.648828
PRIMER_LEFT_4_SEQUENCE=CCGTTCACGCTTTTTCTTTT
PRIMER_RIGHT_4_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_4=483,20
PRIMER_RIGHT_4=1334,20
PRIMER_LEFT_4_TM=59.379
PRIMER_RIGHT_4_TM=56.351
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=852
PRIMER_PAIR_5_PENALTY=4.631089
PRIMER_LEFT_5_PENALTY=0.982262
PRIMER_RIGHT_5_PENALTY=3.648828
PRIMER_LEFT_5_SEQUENCE=GCCGAGAGTCTGAAAGGAAC
PRIMER_RIGHT_5_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_5=560,20
PRIMER_RIGHT_5=1334,20
PRIMER_LEFT_5_TM=59.018
PRIMER_RIGHT_5_TM=56.351
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=775
PRIMER_PAIR_6_PENALTY=4.757936
PRIMER_LEFT_6_PENALTY=1.616276
PRIMER_RIGHT_6_PENALTY=3.141660
PRIMER_LEFT_6_SEQUENCE=GCCGTTCACGCTTTTTCTTT
PRIMER_RIGHT_6_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_6=482,20
PRIMER_RIGHT_6=1334,21
PRIMER_LEFT_6_TM=61.616
PRIMER_RIGHT_6_TM=57.858
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=853
PRIMER_PAIR_7_PENALTY=4.850973
PRIMER_LEFT_7_PENALTY=1.616276
PRIMER_RIGHT_7_PENALTY=3.234696
PRIMER_LEFT_7_SEQUENCE=GCCGTTCACGCTTTTTCTTT
PRIMER_RIGHT_7_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_7=482,20
PRIMER_RIGHT_7=1333,20
PRIMER_LEFT_7_TM=61.616
PRIMER_RIGHT_7_TM=56.765
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=852
PRIMER_PAIR_8_PENALTY=5.265104
PRIMER_LEFT_8_PENALTY=1.616276
PRIMER_RIGHT_8_PENALTY=3.648828
PRIMER_LEFT_8_SEQUENCE=GCCGTTCACGCTTTTTCTTT
PRIMER_RIGHT_8_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_8=482,20
PRIMER_RIGHT_8=1334,20
PRIMER_LEFT_8_TM=61.616
PRIMER_RIGHT_8_TM=56.351
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=853
PRIMER_PAIR_9_PENALTY=5.854403
PRIMER_LEFT_9_PENALTY=2.712744
PRIMER_RIGHT_9_PENALTY=3.141660
PRIMER_LEFT_9_SEQUENCE=CTGCCGTTCACGCTTTTTCT
PRIMER_RIGHT_9_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_9=480,20
PRIMER_RIGHT_9=1334,21
PRIMER_LEFT_9_TM=62.713
PRIMER_RIGHT_9_TM=57.858
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=855
PRIMER_PAIR_10_PENALTY=5.947440
PRIMER_LEFT_10_PENALTY=2.712744
PRIMER_RIGHT_10_PENALTY=3.234696
PRIMER_LEFT_10_SEQUENCE=CTGCCGTTCACGCTTTTTCT
PRIMER_RIGHT_10_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_10=480,20
PRIMER_RIGHT_10=1333,20
PRIMER_LEFT_10_TM=62.713
PRIMER_RIGHT_10_TM=56.765
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=854
PRIMER_PAIR_11_PENALTY=6.155880
PRIMER_LEFT_11_PENALTY=3.014220
PRIMER_RIGHT_11_PENALTY=3.141660
PRIMER_LEFT_11_SEQUENCE=GACTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_11_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_11=439,23
PRIMER_RIGHT_11=1334,21
PRIMER_LEFT_11_TM=60.014
PRIMER_RIGHT_11_TM=57.858
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=896
PRIMER_PAIR_12_PENALTY=6.197122
PRIMER_LEFT_12_PENALTY=3.055462
PRIMER_RIGHT_12_PENALTY=3.141660
PRIMER_LEFT_12_SEQUENCE=TGCCGTTCACGCTTTTTCTT
PRIMER_RIGHT_12_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_12=481,20
PRIMER_RIGHT_12=1334,21
PRIMER_LEFT_12_TM=63.055
PRIMER_RIGHT_12_TM=57.858
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=854
PRIMER_PAIR_13_PENALTY=6.248917
PRIMER_LEFT_13_PENALTY=3.014220
PRIMER_RIGHT_13_PENALTY=3.234696
PRIMER_LEFT_13_SEQUENCE=GACTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_13_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_13=439,23
PRIMER_RIGHT_13=1333,20
PRIMER_LEFT_13_TM=60.014
PRIMER_RIGHT_13_TM=56.765
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=895
PRIMER_PAIR_14_PENALTY=6.290159
PRIMER_LEFT_14_PENALTY=3.055462
PRIMER_RIGHT_14_PENALTY=3.234696
PRIMER_LEFT_14_SEQUENCE=TGCCGTTCACGCTTTTTCTT
PRIMER_RIGHT_14_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_14=481,20
PRIMER_RIGHT_14=1333,20
PRIMER_LEFT_14_TM=63.055
PRIMER_RIGHT_14_TM=56.765
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=853
PRIMER_PAIR_15_PENALTY=6.361571
PRIMER_LEFT_15_PENALTY=2.712744
PRIMER_RIGHT_15_PENALTY=3.648828
PRIMER_LEFT_15_SEQUENCE=CTGCCGTTCACGCTTTTTCT
PRIMER_RIGHT_15_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_15=480,20
PRIMER_RIGHT_15=1334,20
PRIMER_LEFT_15_TM=62.713
PRIMER_RIGHT_15_TM=56.351
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=855
PRIMER_PAIR_16_PENALTY=6.421414
PRIMER_LEFT_16_PENALTY=3.279754
PRIMER_RIGHT_16_PENALTY=3.141660
PRIMER_LEFT_16_SEQUENCE=TGACTAGAAATCATGTGGGCTT
PRIMER_RIGHT_16_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_16=438,22
PRIMER_RIGHT_16=1334,21
PRIMER_LEFT_16_TM=58.720
PRIMER_RIGHT_16_TM=57.858
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=897
PRIMER_PAIR_17_PENALTY=6.514450
PRIMER_LEFT_17_PENALTY=3.279754
PRIMER_RIGHT_17_PENALTY=3.234696
PRIMER_LEFT_17_SEQUENCE=TGACTAGAAATCATGTGGGCTT
PRIMER_RIGHT_17_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_17=438,22
PRIMER_RIGHT_17=1333,20
PRIMER_LEFT_17_TM=58.720
PRIMER_RIGHT_17_TM=56.765
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=896
PRIMER_PAIR_18_PENALTY=6.663048
PRIMER_LEFT_18_PENALTY=3.014220
PRIMER_RIGHT_18_PENALTY=3.648828
PRIMER_LEFT_18_SEQUENCE=GACTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_18_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_18=439,23
PRIMER_RIGHT_18=1334,20
PRIMER_LEFT_18_TM=60.014
PRIMER_RIGHT_18_TM=56.351
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=896
PRIMER_PAIR_19_PENALTY=6.704290
PRIMER_LEFT_19_PENALTY=3.055462
PRIMER_RIGHT_19_PENALTY=3.648828
PRIMER_LEFT_19_SEQUENCE=TGCCGTTCACGCTTTTTCTT
PRIMER_RIGHT_19_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_19=481,20
PRIMER_RIGHT_19=1334,20
PRIMER_LEFT_19_TM=63.055
PRIMER_RIGHT_19_TM=56.351
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=854
PRIMER_PAIR_20_PENALTY=6.759107
PRIMER_LEFT_20_PENALTY=3.617447
PRIMER_RIGHT_20_PENALTY=3.141660
PRIMER_LEFT_20_SEQUENCE=CTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_20_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_20=441,21
PRIMER_RIGHT_20=1334,21
PRIMER_LEFT_20_TM=57.383
PRIMER_RIGHT_20_TM=57.858
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.3000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=894
PRIMER_PAIR_21_PENALTY=6.852144
PRIMER_LEFT_21_PENALTY=3.617447
PRIMER_RIGHT_21_PENALTY=3.234696
PRIMER_LEFT_21_SEQUENCE=CTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_21_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_21=441,21
PRIMER_RIGHT_21=1333,20
PRIMER_LEFT_21_TM=57.383
PRIMER_RIGHT_21_TM=56.765
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=893
PRIMER_PAIR_22_PENALTY=6.860283
PRIMER_LEFT_22_PENALTY=3.718624
PRIMER_RIGHT_22_PENALTY=3.141660
PRIMER_LEFT_22_SEQUENCE=TGACTAGAAATCATGTGGGCT
PRIMER_RIGHT_22_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_22=438,21
PRIMER_RIGHT_22=1334,21
PRIMER_LEFT_22_TM=57.281
PRIMER_RIGHT_22_TM=57.858
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=10.9000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=897
PRIMER_PAIR_23_PENALTY=6.873333
PRIMER_LEFT_23_PENALTY=3.731673
PRIMER_RIGHT_23_PENALTY=3.141660
PRIMER_LEFT_23_SEQUENCE=GCCGTTCACGCTTTTTCTTTT
PRIMER_RIGHT_23_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_23=482,21
PRIMER_RIGHT_23=1334,21
PRIMER_LEFT_23_TM=62.732
PRIMER_RIGHT_23_TM=57.858
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=853
PRIMER_PAIR_24_PENALTY=6.874299
PRIMER_LEFT_24_PENALTY=0.982262
PRIMER_RIGHT_24_PENALTY=5.892038
PRIMER_LEFT_24_SEQUENCE=GCCGAGAGTCTGAAAGGAAC
PRIMER_RIGHT_24_SEQUENCE=CACACAGAGTTAAAAGGCCTAAACA
PRIMER_LEFT_24=560,20
PRIMER_RIGHT_24=1559,25
PRIMER_LEFT_24_TM=59.018
PRIMER_RIGHT_24_TM=60.892
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=1000
PRIMER_PAIR_25_PENALTY=6.877860
PRIMER_LEFT_25_PENALTY=3.736200
PRIMER_RIGHT_25_PENALTY=3.141660
PRIMER_LEFT_25_SEQUENCE=ACTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_25_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_25=440,22
PRIMER_RIGHT_25=1334,21
PRIMER_LEFT_25_TM=58.264
PRIMER_RIGHT_25_TM=57.858
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=895
PRIMER_PAIR_26_PENALTY=6.878255
PRIMER_LEFT_26_PENALTY=3.736595
PRIMER_RIGHT_26_PENALTY=3.141660
PRIMER_LEFT_26_SEQUENCE=TAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_26_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_26=442,20
PRIMER_RIGHT_26=1334,21
PRIMER_LEFT_26_TM=56.263
PRIMER_RIGHT_26_TM=57.858
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=893
PRIMER_PAIR_27_PENALTY=6.928582
PRIMER_LEFT_27_PENALTY=3.279754
PRIMER_RIGHT_27_PENALTY=3.648828
PRIMER_LEFT_27_SEQUENCE=TGACTAGAAATCATGTGGGCTT
PRIMER_RIGHT_27_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_27=438,22
PRIMER_RIGHT_27=1334,20
PRIMER_LEFT_27_TM=58.720
PRIMER_RIGHT_27_TM=56.351
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=897
PRIMER_PAIR_28_PENALTY=6.953320
PRIMER_LEFT_28_PENALTY=3.718624
PRIMER_RIGHT_28_PENALTY=3.234696
PRIMER_LEFT_28_SEQUENCE=TGACTAGAAATCATGTGGGCT
PRIMER_RIGHT_28_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_28=438,21
PRIMER_RIGHT_28=1333,20
PRIMER_LEFT_28_TM=57.281
PRIMER_RIGHT_28_TM=56.765
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=10.9000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=896
PRIMER_PAIR_29_PENALTY=6.966370
PRIMER_LEFT_29_PENALTY=3.731673
PRIMER_RIGHT_29_PENALTY=3.234696
PRIMER_LEFT_29_SEQUENCE=GCCGTTCACGCTTTTTCTTTT
PRIMER_RIGHT_29_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_29=482,21
PRIMER_RIGHT_29=1333,20
PRIMER_LEFT_29_TM=62.732
PRIMER_RIGHT_29_TM=56.765
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.3000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=852
PRIMER_PAIR_30_PENALTY=6.970897
PRIMER_LEFT_30_PENALTY=3.736200
PRIMER_RIGHT_30_PENALTY=3.234696
PRIMER_LEFT_30_SEQUENCE=ACTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_30_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_30=440,22
PRIMER_RIGHT_30=1333,20
PRIMER_LEFT_30_TM=58.264
PRIMER_RIGHT_30_TM=56.765
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=894
PRIMER_PAIR_31_PENALTY=6.971292
PRIMER_LEFT_31_PENALTY=3.736595
PRIMER_RIGHT_31_PENALTY=3.234696
PRIMER_LEFT_31_SEQUENCE=TAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_31_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_31=442,20
PRIMER_RIGHT_31=1333,20
PRIMER_LEFT_31_TM=56.263
PRIMER_RIGHT_31_TM=56.765
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.3000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=892
PRIMER_PAIR_32_PENALTY=7.266275
PRIMER_LEFT_32_PENALTY=3.617447
PRIMER_RIGHT_32_PENALTY=3.648828
PRIMER_LEFT_32_SEQUENCE=CTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_32_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_32=441,21
PRIMER_RIGHT_32=1334,20
PRIMER_LEFT_32_TM=57.383
PRIMER_RIGHT_32_TM=56.351
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=9.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=894
PRIMER_PAIR_33_PENALTY=7.300679
PRIMER_LEFT_33_PENALTY=4.159019
PRIMER_RIGHT_33_PENALTY=3.141660
PRIMER_LEFT_33_SEQUENCE=ATCCTGCCGTTCACGCTTTT
PRIMER_RIGHT_33_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_33=477,20
PRIMER_RIGHT_33=1334,21
PRIMER_LEFT_33_TM=64.159
PRIMER_RIGHT_33_TM=57.858
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=858
PRIMER_PAIR_34_PENALTY=7.300679
PRIMER_LEFT_34_PENALTY=4.159019
PRIMER_RIGHT_34_PENALTY=3.141660
PRIMER_LEFT_34_SEQUENCE=AATCCTGCCGTTCACGCTTT
PRIMER_RIGHT_34_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_34=476,20
PRIMER_RIGHT_34=1334,21
PRIMER_LEFT_34_TM=64.159
PRIMER_RIGHT_34_TM=57.858
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=859
PRIMER_PAIR_35_PENALTY=7.353309
PRIMER_LEFT_35_PENALTY=4.211649
PRIMER_RIGHT_35_PENALTY=3.141660
PRIMER_LEFT_35_SEQUENCE=CCGTTCACGCTTTTTCTTTTTC
PRIMER_RIGHT_35_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_35=483,22
PRIMER_RIGHT_35=1334,21
PRIMER_LEFT_35_TM=62.212
PRIMER_RIGHT_35_TM=57.858
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.3000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=852
PRIMER_PAIR_36_PENALTY=7.367451
PRIMER_LEFT_36_PENALTY=3.718624
PRIMER_RIGHT_36_PENALTY=3.648828
PRIMER_LEFT_36_SEQUENCE=TGACTAGAAATCATGTGGGCT
PRIMER_RIGHT_36_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_36=438,21
PRIMER_RIGHT_36=1334,20
PRIMER_LEFT_36_TM=57.281
PRIMER_RIGHT_36_TM=56.351
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=897
PRIMER_PAIR_37_PENALTY=7.380501
PRIMER_LEFT_37_PENALTY=3.731673
PRIMER_RIGHT_37_PENALTY=3.648828
PRIMER_LEFT_37_SEQUENCE=GCCGTTCACGCTTTTTCTTTT
PRIMER_RIGHT_37_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_37=482,21
PRIMER_RIGHT_37=1334,20
PRIMER_LEFT_37_TM=62.732
PRIMER_RIGHT_37_TM=56.351
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.3000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=853
PRIMER_PAIR_38_PENALTY=7.385028
PRIMER_LEFT_38_PENALTY=3.736200
PRIMER_RIGHT_38_PENALTY=3.648828
PRIMER_LEFT_38_SEQUENCE=ACTAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_38_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_38=440,22
PRIMER_RIGHT_38=1334,20
PRIMER_LEFT_38_TM=58.264
PRIMER_RIGHT_38_TM=56.351
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.3000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=895
PRIMER_PAIR_39_PENALTY=7.385423
PRIMER_LEFT_39_PENALTY=3.736595
PRIMER_RIGHT_39_PENALTY=3.648828
PRIMER_LEFT_39_SEQUENCE=TAGAAATCATGTGGGCTTTG
PRIMER_RIGHT_39_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_39=442,20
PRIMER_RIGHT_39=1334,20
PRIMER_LEFT_39_TM=56.263
PRIMER_RIGHT_39_TM=56.351
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=893
PRIMER_PAIR_40_PENALTY=7.393716
PRIMER_LEFT_40_PENALTY=4.159019
PRIMER_RIGHT_40_PENALTY=3.234696
PRIMER_LEFT_40_SEQUENCE=ATCCTGCCGTTCACGCTTTT
PRIMER_RIGHT_40_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_40=477,20
PRIMER_RIGHT_40=1333,20
PRIMER_LEFT_40_TM=64.159
PRIMER_RIGHT_40_TM=56.765
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.3000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=857
PRIMER_PAIR_41_PENALTY=7.393716
PRIMER_LEFT_41_PENALTY=4.159019
PRIMER_RIGHT_41_PENALTY=3.234696
PRIMER_LEFT_41_SEQUENCE=AATCCTGCCGTTCACGCTTT
PRIMER_RIGHT_41_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_41=476,20
PRIMER_RIGHT_41=1333,20
PRIMER_LEFT_41_TM=64.159
PRIMER_RIGHT_41_TM=56.765
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=858
PRIMER_PAIR_42_PENALTY=7.446345
PRIMER_LEFT_42_PENALTY=4.211649
PRIMER_RIGHT_42_PENALTY=3.234696
PRIMER_LEFT_42_SEQUENCE=CCGTTCACGCTTTTTCTTTTTC
PRIMER_RIGHT_42_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_42=483,22
PRIMER_RIGHT_42=1333,20
PRIMER_LEFT_42_TM=62.212
PRIMER_RIGHT_42_TM=56.765
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=9.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=851
PRIMER_PAIR_43_PENALTY=7.448139
PRIMER_LEFT_43_PENALTY=4.306479
PRIMER_RIGHT_43_PENALTY=3.141660
PRIMER_LEFT_43_SEQUENCE=ATGACTAGAAATCATGTGGGCT
PRIMER_RIGHT_43_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_43=437,22
PRIMER_RIGHT_43=1334,21
PRIMER_LEFT_43_TM=57.694
PRIMER_RIGHT_43_TM=57.858
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.9000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=898
PRIMER_PAIR_44_PENALTY=7.541176
PRIMER_LEFT_44_PENALTY=4.306479
PRIMER_RIGHT_44_PENALTY=3.234696
PRIMER_LEFT_44_SEQUENCE=ATGACTAGAAATCATGTGGGCT
PRIMER_RIGHT_44_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_44=437,22
PRIMER_RIGHT_44=1333,20
PRIMER_LEFT_44_TM=57.694
PRIMER_RIGHT_44_TM=56.765
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.9000
PRIMER_RIGHT_44_END_STABILITY=9.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=897
PRIMER_PAIR_45_PENALTY=7.765836
PRIMER_LEFT_45_PENALTY=4.624177
PRIMER_RIGHT_45_PENALTY=3.141660
PRIMER_LEFT_45_SEQUENCE=GACTAGAAATCATGTGGGCTTT
PRIMER_RIGHT_45_SEQUENCE=AGGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_45=439,22
PRIMER_RIGHT_45=1334,21
PRIMER_LEFT_45_TM=57.376
PRIMER_RIGHT_45_TM=57.858
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=896
PRIMER_PAIR_46_PENALTY=7.807847
PRIMER_LEFT_46_PENALTY=4.159019
PRIMER_RIGHT_46_PENALTY=3.648828
PRIMER_LEFT_46_SEQUENCE=ATCCTGCCGTTCACGCTTTT
PRIMER_RIGHT_46_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_46=477,20
PRIMER_RIGHT_46=1334,20
PRIMER_LEFT_46_TM=64.159
PRIMER_RIGHT_46_TM=56.351
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=9.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=858
PRIMER_PAIR_47_PENALTY=7.807847
PRIMER_LEFT_47_PENALTY=4.159019
PRIMER_RIGHT_47_PENALTY=3.648828
PRIMER_LEFT_47_SEQUENCE=AATCCTGCCGTTCACGCTTT
PRIMER_RIGHT_47_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_47=476,20
PRIMER_RIGHT_47=1334,20
PRIMER_LEFT_47_TM=64.159
PRIMER_RIGHT_47_TM=56.351
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=859
PRIMER_PAIR_48_PENALTY=7.858873
PRIMER_LEFT_48_PENALTY=4.624177
PRIMER_RIGHT_48_PENALTY=3.234696
PRIMER_LEFT_48_SEQUENCE=GACTAGAAATCATGTGGGCTTT
PRIMER_RIGHT_48_SEQUENCE=GGTTGTAGGAGTGTTGGCTT
PRIMER_LEFT_48=439,22
PRIMER_RIGHT_48=1333,20
PRIMER_LEFT_48_TM=57.376
PRIMER_RIGHT_48_TM=56.765
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=895
PRIMER_PAIR_49_PENALTY=7.860476
PRIMER_LEFT_49_PENALTY=4.211649
PRIMER_RIGHT_49_PENALTY=3.648828
PRIMER_LEFT_49_SEQUENCE=CCGTTCACGCTTTTTCTTTTTC
PRIMER_RIGHT_49_SEQUENCE=AGGTTGTAGGAGTGTTGGCT
PRIMER_LEFT_49=483,22
PRIMER_RIGHT_49=1334,20
PRIMER_LEFT_49_TM=62.212
PRIMER_RIGHT_49_TM=56.351
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=852
=
SEQUENCE_ID=MAGIC.175_3_9625568_9625650_zu-0_sf-2_12341_852850_TRUE
SEQUENCE_TARGET=950,182
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNAATATCANGCGNTTAANTTTTGTTTNTCNCNATTNNNTTTNTTATNATAATNNNNNNNNNNNNGTATNTAGGTGATATGANAAAAANATAAAAAANGTNAATGANAANAAAGNTGNTTTTAAANTATANTANNNGTTGATTAANAGTGGTTTTACNCANAAATAGAGAANTGAGAGATTATTCGAAGTTTTAANANGTTTGAANAAAATTGTTTGTACATGTTGAAAAANCANANCANGAATTGTTANGATNANAATTGATNGTTCGAACCAAAANTAATGTTANNAAACCATATATGAAACANGGTTTNCCATNATTCATTTATCNNNNNNNNGAANTAGTNNNNNNNTATTTTTCAAACTTCATAAGAAGTNAGTTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCANNTGTTCTTNAATGATACNACNTTGANGTTNTTTTCATNCTGCTTTNTTNAATTCAACTTTGAANGNACATAGTTATCAATTNGAATTAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_1023905_1024035_can-0_edi-0_399_616318_TRUE
SEQUENCE_TARGET=950,230
SEQUENCE_TEMPLATE=GGGTATTTGGGTTGGGTCTATTNTCATAGGNCCATGTGAAATTAGGCCCACAGTGGCTGAAGTGAAGCAATGTGTAATAGAANCATTTTCTTTGTCTAGTAATATNGTTTTGTGTTTGCTAAACGTACCTAGNATATCNATGTATTTTGGCATCCACCATTCCTTNGNATTCACAACCACNAGGATGAAATNGCGGTCNGGGTATACCNAGTTNGCCGTGATAAAAAGNGGNATGAAACCATCGATAAAATCNTGATAAATNCTTCCGGTGTAACCTCCAGCACTGAACNCAATGNCCGGNGAATCATGTGTTATNNNNNNNNNNNNNNNNNNNNNNNATGGGCNTGANGNTAGTTTTAGCTCTCTGATCNTAGGCATGATCCANTTTTCTGNTTTTCTTGGNTATGGCCTGATTTTCTCGACAAGTGGTGCNNNTGTGGTNANTGTTNGGTCNATNAGAGTTANANTTCCGGTTTTNGNGTTGAGAATACATGAGCCGTTGATGGAGCAAAGATCNTAACTAGTGTGGGATCGGTCACATGTGATTTTTGCTGATGNAGCCATCACTTNNGGGTTTTGCTTTGTTCTTNTNGATTCCCATCTTTGTTGTAACGTACCTACAGAGGAAANTTNTTTNANNTNNTNNNNNNAAACNNNNNGTNAANNTNTNTAAGANANNANTNAGNAANTANTAAAANAGNTTTNNNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNTNNTNNATGNANNANNANTNCANATGNTTANAANNNAANNNNNNNNNNNAANTNAANNCNCNTNNAANANATTANNNNGNNTTANNNTNNTNANANANCNTNNCGNNNTAANTATNNANNANNCNATNANGTAANCTNTTANNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAANNNNNANNTNACANNNNNNNAANTNNNATTANANNNAAAANTCATTANNNNNNNNNNTTAGTTGCTTCTTTTGANCGGANTTTAGAATTANTGNNGTTTCGTAATGTTAGCATTTAGATTCAAATCCAGCCAAGTATGTGTGAGTTATTGCAAAAATGTTGACTNGATCGTTAAAACANTAGGTAATATAAAAGNAAAAGTGTTTGATCNTAGCCTACGACTCAANCAAGAAGTTNAAGNTTTATNTGGTTGAATCGAAGANGTAATTTGNTATNTACATACCTTGAGAGAAACTTNACGAANGAAACAATTGACGATCGNAGACGGAGGAGAAAGCAACATATATAACCATAACGATCACCAACGCCACCGAATAACTCTTCGACGTCNTTTTNNTAACCATGNNTTATATGCANATCNACCTTTAAGAATCCTTCAATNNAAGAGAAAAGAAAGAANAANNTTGTAAGTCNANTANNGGNTGAANAACAGAAGAAGATAGAATGNAAACGAGANCANTGATTCAGTATANNTGTANTTTTATCNAGAAGACTTTTCTCTTACAAAATATTTTTAATTGTTTGNAANNNTTTTTGCNANAAAAANAAAANNNTTTNCNTAATGCTGAGGNATGGGAATACTATAATAGAACAAATANGTNATAACATATCTCAAANGGAGTNNACGTTTACAGCTNTTTACTCNNNTTGCTANATCATAAAACAGTGAAAGAGATANTTTTTTTAGGTTTTAAGTNNGANANNNNNNNNNNNNNAANANAANNNNNNNNNATCTTATTNTACAGCTCAAAANTCACAAATCATTACAATANTACATAATCAATCAACCGTTTATGTCCATAAATATTTTAGCNTTCTTATATGCTTCATTCATATGTTTNCTAAACCGATTCACATCTAANCTCACATCTTNTTCTTTTANATANACTTTCATTTTANTCGCATTCCAANCCATTCCTCNATAAGCAATNGGNTCTTTCAAAACCAAATCATCTCTNNTATANTTCTCTATCAAACTNCTCTCTTCCACATTNACCTTATACTCCANATACTCCAACTTCATTGTCTTAGCTGGCGATTCGTAACACGGTTTAGACGCCCAACCTAGCCCAAGCGGAACAACTTGAACAAATANTGAACCGGG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.459379
PRIMER_LEFT_0_PENALTY=0.327117
PRIMER_RIGHT_0_PENALTY=0.132262
PRIMER_LEFT_0_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_0_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_0=488,20
PRIMER_RIGHT_0=1346,20
PRIMER_LEFT_0_TM=59.673
PRIMER_RIGHT_0_TM=60.132
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=859
PRIMER_PAIR_1_PENALTY=1.113040
PRIMER_LEFT_1_PENALTY=0.327117
PRIMER_RIGHT_1_PENALTY=0.785923
PRIMER_LEFT_1_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_1_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_1=488,20
PRIMER_RIGHT_1=1332,20
PRIMER_LEFT_1_TM=59.673
PRIMER_RIGHT_1_TM=60.786
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=845
PRIMER_PAIR_2_PENALTY=1.128746
PRIMER_LEFT_2_PENALTY=0.327117
PRIMER_RIGHT_2_PENALTY=0.801629
PRIMER_LEFT_2_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_2_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_2=488,20
PRIMER_RIGHT_2=1348,20
PRIMER_LEFT_2_TM=59.673
PRIMER_RIGHT_2_TM=59.198
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=861
PRIMER_PAIR_3_PENALTY=1.606871
PRIMER_LEFT_3_PENALTY=0.327117
PRIMER_RIGHT_3_PENALTY=1.279754
PRIMER_LEFT_3_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_3_SEQUENCE=AAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_3=488,20
PRIMER_RIGHT_3=1347,20
PRIMER_LEFT_3_TM=59.673
PRIMER_RIGHT_3_TM=58.720
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=860
PRIMER_PAIR_4_PENALTY=1.723828
PRIMER_LEFT_4_PENALTY=1.713283
PRIMER_RIGHT_4_PENALTY=0.010545
PRIMER_LEFT_4_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_4_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_4=482,21
PRIMER_RIGHT_4=1306,20
PRIMER_LEFT_4_TM=59.287
PRIMER_RIGHT_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=825
PRIMER_PAIR_5_PENALTY=1.823992
PRIMER_LEFT_5_PENALTY=0.327117
PRIMER_RIGHT_5_PENALTY=1.496875
PRIMER_LEFT_5_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_5_SEQUENCE=GAAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_5=488,20
PRIMER_RIGHT_5=1348,21
PRIMER_LEFT_5_TM=59.673
PRIMER_RIGHT_5_TM=60.497
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=861
PRIMER_PAIR_6_PENALTY=1.845545
PRIMER_LEFT_6_PENALTY=1.713283
PRIMER_RIGHT_6_PENALTY=0.132262
PRIMER_LEFT_6_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_6_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_6=482,21
PRIMER_RIGHT_6=1346,20
PRIMER_LEFT_6_TM=59.287
PRIMER_RIGHT_6_TM=60.132
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=865
PRIMER_PAIR_7_PENALTY=1.974890
PRIMER_LEFT_7_PENALTY=0.327117
PRIMER_RIGHT_7_PENALTY=1.647773
PRIMER_LEFT_7_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_7_SEQUENCE=CGTTGGTGATCGTTATGGTT
PRIMER_LEFT_7=488,20
PRIMER_RIGHT_7=1331,20
PRIMER_LEFT_7_TM=59.673
PRIMER_RIGHT_7_TM=58.352
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=844
PRIMER_PAIR_8_PENALTY=1.995775
PRIMER_LEFT_8_PENALTY=0.327117
PRIMER_RIGHT_8_PENALTY=1.668658
PRIMER_LEFT_8_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_8_SEQUENCE=AGTTATTCGGTGGCGTTGGT
PRIMER_LEFT_8=488,20
PRIMER_RIGHT_8=1344,20
PRIMER_LEFT_8_TM=59.673
PRIMER_RIGHT_8_TM=61.669
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=857
PRIMER_PAIR_9_PENALTY=2.225735
PRIMER_LEFT_9_PENALTY=2.215190
PRIMER_RIGHT_9_PENALTY=0.010545
PRIMER_LEFT_9_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_9_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_9=481,21
PRIMER_RIGHT_9=1306,20
PRIMER_LEFT_9_TM=58.785
PRIMER_RIGHT_9_TM=59.989
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.7000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=826
PRIMER_PAIR_10_PENALTY=2.347452
PRIMER_LEFT_10_PENALTY=2.215190
PRIMER_RIGHT_10_PENALTY=0.132262
PRIMER_LEFT_10_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_10_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_10=481,21
PRIMER_RIGHT_10=1346,20
PRIMER_LEFT_10_TM=58.785
PRIMER_RIGHT_10_TM=60.132
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.7000
PRIMER_RIGHT_10_END_STABILITY=8.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=866
PRIMER_PAIR_11_PENALTY=2.373308
PRIMER_LEFT_11_PENALTY=2.362762
PRIMER_RIGHT_11_PENALTY=0.010545
PRIMER_LEFT_11_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_11_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_11=480,22
PRIMER_RIGHT_11=1306,20
PRIMER_LEFT_11_TM=59.637
PRIMER_RIGHT_11_TM=59.989
PRIMER_LEFT_11_GC_PERCENT=45.455
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.7000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=827
PRIMER_PAIR_12_PENALTY=2.425074
PRIMER_LEFT_12_PENALTY=1.713283
PRIMER_RIGHT_12_PENALTY=0.711791
PRIMER_LEFT_12_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_12_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_12=482,21
PRIMER_RIGHT_12=1307,20
PRIMER_LEFT_12_TM=59.287
PRIMER_RIGHT_12_TM=59.288
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=826
PRIMER_PAIR_13_PENALTY=2.495024
PRIMER_LEFT_13_PENALTY=2.362762
PRIMER_RIGHT_13_PENALTY=0.132262
PRIMER_LEFT_13_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_13_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_13=480,22
PRIMER_RIGHT_13=1346,20
PRIMER_LEFT_13_TM=59.637
PRIMER_RIGHT_13_TM=60.132
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.7000
PRIMER_RIGHT_13_END_STABILITY=8.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=867
PRIMER_PAIR_14_PENALTY=2.499206
PRIMER_LEFT_14_PENALTY=1.713283
PRIMER_RIGHT_14_PENALTY=0.785923
PRIMER_LEFT_14_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_14_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_14=482,21
PRIMER_RIGHT_14=1332,20
PRIMER_LEFT_14_TM=59.287
PRIMER_RIGHT_14_TM=60.786
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.8000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=851
PRIMER_PAIR_15_PENALTY=2.514912
PRIMER_LEFT_15_PENALTY=1.713283
PRIMER_RIGHT_15_PENALTY=0.801629
PRIMER_LEFT_15_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_15_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_15=482,21
PRIMER_RIGHT_15=1348,20
PRIMER_LEFT_15_TM=59.287
PRIMER_RIGHT_15_TM=59.198
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=11.1000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=867
PRIMER_PAIR_16_PENALTY=2.648011
PRIMER_LEFT_16_PENALTY=2.637466
PRIMER_RIGHT_16_PENALTY=0.010545
PRIMER_LEFT_16_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_16_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_16=481,22
PRIMER_RIGHT_16=1306,20
PRIMER_LEFT_16_TM=60.637
PRIMER_RIGHT_16_TM=59.989
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=826
PRIMER_PAIR_17_PENALTY=2.711413
PRIMER_LEFT_17_PENALTY=0.327117
PRIMER_RIGHT_17_PENALTY=2.384296
PRIMER_LEFT_17_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_17_SEQUENCE=AAGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_17=488,20
PRIMER_RIGHT_17=1347,21
PRIMER_LEFT_17_TM=59.673
PRIMER_RIGHT_17_TM=61.384
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=860
PRIMER_PAIR_18_PENALTY=2.729760
PRIMER_LEFT_18_PENALTY=2.719215
PRIMER_RIGHT_18_PENALTY=0.010545
PRIMER_LEFT_18_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_18_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_18=482,20
PRIMER_RIGHT_18=1306,20
PRIMER_LEFT_18_TM=57.281
PRIMER_RIGHT_18_TM=59.989
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.7000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=825
PRIMER_PAIR_19_PENALTY=2.769728
PRIMER_LEFT_19_PENALTY=2.637466
PRIMER_RIGHT_19_PENALTY=0.132262
PRIMER_LEFT_19_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_19_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_19=481,22
PRIMER_RIGHT_19=1346,20
PRIMER_LEFT_19_TM=60.637
PRIMER_RIGHT_19_TM=60.132
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=866
PRIMER_PAIR_20_PENALTY=2.851477
PRIMER_LEFT_20_PENALTY=2.719215
PRIMER_RIGHT_20_PENALTY=0.132262
PRIMER_LEFT_20_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_20_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_20=482,20
PRIMER_RIGHT_20=1346,20
PRIMER_LEFT_20_TM=57.281
PRIMER_RIGHT_20_TM=60.132
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.7000
PRIMER_RIGHT_20_END_STABILITY=8.7000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=865
PRIMER_PAIR_21_PENALTY=2.926981
PRIMER_LEFT_21_PENALTY=2.215190
PRIMER_RIGHT_21_PENALTY=0.711791
PRIMER_LEFT_21_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_21_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_21=481,21
PRIMER_RIGHT_21=1307,20
PRIMER_LEFT_21_TM=58.785
PRIMER_RIGHT_21_TM=59.288
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.7000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=827
PRIMER_PAIR_22_PENALTY=2.974509
PRIMER_LEFT_22_PENALTY=1.713283
PRIMER_RIGHT_22_PENALTY=1.261226
PRIMER_LEFT_22_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_22_SEQUENCE=ATGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_22=482,21
PRIMER_RIGHT_22=1307,21
PRIMER_LEFT_22_TM=59.287
PRIMER_RIGHT_22_TM=60.261
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=826
PRIMER_PAIR_23_PENALTY=2.993037
PRIMER_LEFT_23_PENALTY=1.713283
PRIMER_RIGHT_23_PENALTY=1.279754
PRIMER_LEFT_23_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_23_SEQUENCE=AAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_23=482,21
PRIMER_RIGHT_23=1347,20
PRIMER_LEFT_23_TM=59.287
PRIMER_RIGHT_23_TM=58.720
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=9.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=866
PRIMER_PAIR_24_PENALTY=3.001113
PRIMER_LEFT_24_PENALTY=2.215190
PRIMER_RIGHT_24_PENALTY=0.785923
PRIMER_LEFT_24_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_24_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_24=481,21
PRIMER_RIGHT_24=1332,20
PRIMER_LEFT_24_TM=58.785
PRIMER_RIGHT_24_TM=60.786
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.7000
PRIMER_RIGHT_24_END_STABILITY=7.8000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=852
PRIMER_PAIR_25_PENALTY=3.011470
PRIMER_LEFT_25_PENALTY=0.327117
PRIMER_RIGHT_25_PENALTY=2.684353
PRIMER_LEFT_25_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_25_SEQUENCE=GAGTTATTCGGTGGCGTTGG
PRIMER_LEFT_25=488,20
PRIMER_RIGHT_25=1345,20
PRIMER_LEFT_25_TM=59.673
PRIMER_RIGHT_25_TM=62.684
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=858
PRIMER_PAIR_26_PENALTY=3.016819
PRIMER_LEFT_26_PENALTY=2.215190
PRIMER_RIGHT_26_PENALTY=0.801629
PRIMER_LEFT_26_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_26_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_26=481,21
PRIMER_RIGHT_26=1348,20
PRIMER_LEFT_26_TM=58.785
PRIMER_RIGHT_26_TM=59.198
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.7000
PRIMER_RIGHT_26_END_STABILITY=11.1000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=868
PRIMER_PAIR_27_PENALTY=3.058815
PRIMER_LEFT_27_PENALTY=0.327117
PRIMER_RIGHT_27_PENALTY=2.731698
PRIMER_LEFT_27_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_27_SEQUENCE=GTCGAAGAGTTATTCGGTGG
PRIMER_LEFT_27=488,20
PRIMER_RIGHT_27=1351,20
PRIMER_LEFT_27_TM=59.673
PRIMER_RIGHT_27_TM=57.268
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=864
PRIMER_PAIR_28_PENALTY=3.074553
PRIMER_LEFT_28_PENALTY=2.362762
PRIMER_RIGHT_28_PENALTY=0.711791
PRIMER_LEFT_28_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_28_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_28=480,22
PRIMER_RIGHT_28=1307,20
PRIMER_LEFT_28_TM=59.637
PRIMER_RIGHT_28_TM=59.288
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.7000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=828
PRIMER_PAIR_29_PENALTY=3.143647
PRIMER_LEFT_29_PENALTY=0.327117
PRIMER_RIGHT_29_PENALTY=2.816530
PRIMER_LEFT_29_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_29_SEQUENCE=TGGCGTTGGTGATCGTTATG
PRIMER_LEFT_29=488,20
PRIMER_RIGHT_29=1334,20
PRIMER_LEFT_29_TM=59.673
PRIMER_RIGHT_29_TM=62.817
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=847
PRIMER_PAIR_30_PENALTY=3.148686
PRIMER_LEFT_30_PENALTY=2.362762
PRIMER_RIGHT_30_PENALTY=0.785923
PRIMER_LEFT_30_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_30_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_30=480,22
PRIMER_RIGHT_30=1332,20
PRIMER_LEFT_30_TM=59.637
PRIMER_RIGHT_30_TM=60.786
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.7000
PRIMER_RIGHT_30_END_STABILITY=7.8000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=853
PRIMER_PAIR_31_PENALTY=3.164391
PRIMER_LEFT_31_PENALTY=2.362762
PRIMER_RIGHT_31_PENALTY=0.801629
PRIMER_LEFT_31_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_31_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_31=480,22
PRIMER_RIGHT_31=1348,20
PRIMER_LEFT_31_TM=59.637
PRIMER_RIGHT_31_TM=59.198
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.7000
PRIMER_RIGHT_31_END_STABILITY=11.1000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=869
PRIMER_PAIR_32_PENALTY=3.210158
PRIMER_LEFT_32_PENALTY=1.713283
PRIMER_RIGHT_32_PENALTY=1.496875
PRIMER_LEFT_32_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_32_SEQUENCE=GAAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_32=482,21
PRIMER_RIGHT_32=1348,21
PRIMER_LEFT_32_TM=59.287
PRIMER_RIGHT_32_TM=60.497
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=867
PRIMER_PAIR_33_PENALTY=3.349257
PRIMER_LEFT_33_PENALTY=2.637466
PRIMER_RIGHT_33_PENALTY=0.711791
PRIMER_LEFT_33_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_33_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_33=481,22
PRIMER_RIGHT_33=1307,20
PRIMER_LEFT_33_TM=60.637
PRIMER_RIGHT_33_TM=59.288
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=827
PRIMER_PAIR_34_PENALTY=3.361056
PRIMER_LEFT_34_PENALTY=1.713283
PRIMER_RIGHT_34_PENALTY=1.647773
PRIMER_LEFT_34_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_34_SEQUENCE=CGTTGGTGATCGTTATGGTT
PRIMER_LEFT_34=482,21
PRIMER_RIGHT_34=1331,20
PRIMER_LEFT_34_TM=59.287
PRIMER_RIGHT_34_TM=58.352
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=850
PRIMER_PAIR_35_PENALTY=3.362235
PRIMER_LEFT_35_PENALTY=0.327117
PRIMER_RIGHT_35_PENALTY=3.035118
PRIMER_LEFT_35_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_35_SEQUENCE=GCGTTGGTGATCGTTATGGTT
PRIMER_LEFT_35=488,20
PRIMER_RIGHT_35=1332,21
PRIMER_LEFT_35_TM=59.673
PRIMER_RIGHT_35_TM=62.035
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=845
PRIMER_PAIR_36_PENALTY=3.381941
PRIMER_LEFT_36_PENALTY=1.713283
PRIMER_RIGHT_36_PENALTY=1.668658
PRIMER_LEFT_36_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_36_SEQUENCE=AGTTATTCGGTGGCGTTGGT
PRIMER_LEFT_36=482,21
PRIMER_RIGHT_36=1344,20
PRIMER_LEFT_36_TM=59.287
PRIMER_RIGHT_36_TM=61.669
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=863
PRIMER_PAIR_37_PENALTY=3.387251
PRIMER_LEFT_37_PENALTY=1.713283
PRIMER_RIGHT_37_PENALTY=1.673968
PRIMER_LEFT_37_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_37_SEQUENCE=TATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_37=482,21
PRIMER_RIGHT_37=1308,21
PRIMER_LEFT_37_TM=59.287
PRIMER_RIGHT_37_TM=59.326
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=827
PRIMER_PAIR_38_PENALTY=3.423389
PRIMER_LEFT_38_PENALTY=2.637466
PRIMER_RIGHT_38_PENALTY=0.785923
PRIMER_LEFT_38_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_38_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_38=481,22
PRIMER_RIGHT_38=1332,20
PRIMER_LEFT_38_TM=60.637
PRIMER_RIGHT_38_TM=60.786
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=7.8000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=852
PRIMER_PAIR_39_PENALTY=3.431006
PRIMER_LEFT_39_PENALTY=2.719215
PRIMER_RIGHT_39_PENALTY=0.711791
PRIMER_LEFT_39_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_39_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_39=482,20
PRIMER_RIGHT_39=1307,20
PRIMER_LEFT_39_TM=57.281
PRIMER_RIGHT_39_TM=59.288
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.7000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=826
PRIMER_PAIR_40_PENALTY=3.439095
PRIMER_LEFT_40_PENALTY=2.637466
PRIMER_RIGHT_40_PENALTY=0.801629
PRIMER_LEFT_40_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_40_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_40=481,22
PRIMER_RIGHT_40=1348,20
PRIMER_LEFT_40_TM=60.637
PRIMER_RIGHT_40_TM=59.198
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=11.1000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=868
PRIMER_PAIR_41_PENALTY=3.476416
PRIMER_LEFT_41_PENALTY=2.215190
PRIMER_RIGHT_41_PENALTY=1.261226
PRIMER_LEFT_41_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_41_SEQUENCE=ATGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_41=481,21
PRIMER_RIGHT_41=1307,21
PRIMER_LEFT_41_TM=58.785
PRIMER_RIGHT_41_TM=60.261
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.7000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=827
PRIMER_PAIR_42_PENALTY=3.494944
PRIMER_LEFT_42_PENALTY=2.215190
PRIMER_RIGHT_42_PENALTY=1.279754
PRIMER_LEFT_42_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_42_SEQUENCE=AAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_42=481,21
PRIMER_RIGHT_42=1347,20
PRIMER_LEFT_42_TM=58.785
PRIMER_RIGHT_42_TM=58.720
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.7000
PRIMER_RIGHT_42_END_STABILITY=9.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=867
PRIMER_PAIR_43_PENALTY=3.505138
PRIMER_LEFT_43_PENALTY=2.719215
PRIMER_RIGHT_43_PENALTY=0.785923
PRIMER_LEFT_43_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_43_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_43=482,20
PRIMER_RIGHT_43=1332,20
PRIMER_LEFT_43_TM=57.281
PRIMER_RIGHT_43_TM=60.786
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.7000
PRIMER_RIGHT_43_END_STABILITY=7.8000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=851
PRIMER_PAIR_44_PENALTY=3.520844
PRIMER_LEFT_44_PENALTY=2.719215
PRIMER_RIGHT_44_PENALTY=0.801629
PRIMER_LEFT_44_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_44_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_44=482,20
PRIMER_RIGHT_44=1348,20
PRIMER_LEFT_44_TM=57.281
PRIMER_RIGHT_44_TM=59.198
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.7000
PRIMER_RIGHT_44_END_STABILITY=11.1000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=867
PRIMER_PAIR_45_PENALTY=3.623988
PRIMER_LEFT_45_PENALTY=2.362762
PRIMER_RIGHT_45_PENALTY=1.261226
PRIMER_LEFT_45_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_45_SEQUENCE=ATGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_45=480,22
PRIMER_RIGHT_45=1307,21
PRIMER_LEFT_45_TM=59.637
PRIMER_RIGHT_45_TM=60.261
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.7000
PRIMER_RIGHT_45_END_STABILITY=8.1000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=828
PRIMER_PAIR_46_PENALTY=3.642516
PRIMER_LEFT_46_PENALTY=2.362762
PRIMER_RIGHT_46_PENALTY=1.279754
PRIMER_LEFT_46_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_46_SEQUENCE=AAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_46=480,22
PRIMER_RIGHT_46=1347,20
PRIMER_LEFT_46_TM=59.637
PRIMER_RIGHT_46_TM=58.720
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.7000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=868
PRIMER_PAIR_47_PENALTY=3.712065
PRIMER_LEFT_47_PENALTY=2.215190
PRIMER_RIGHT_47_PENALTY=1.496875
PRIMER_LEFT_47_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_47_SEQUENCE=GAAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_47=481,21
PRIMER_RIGHT_47=1348,21
PRIMER_LEFT_47_TM=58.785
PRIMER_RIGHT_47_TM=60.497
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.7000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=868
PRIMER_PAIR_48_PENALTY=3.733564
PRIMER_LEFT_48_PENALTY=3.723019
PRIMER_RIGHT_48_PENALTY=0.010545
PRIMER_LEFT_48_SEQUENCE=GAGAATACATGAGCCGTTGA
PRIMER_RIGHT_48_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_48=483,20
PRIMER_RIGHT_48=1306,20
PRIMER_LEFT_48_TM=56.277
PRIMER_RIGHT_48_TM=59.989
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=824
PRIMER_PAIR_49_PENALTY=3.829598
PRIMER_LEFT_49_PENALTY=0.327117
PRIMER_RIGHT_49_PENALTY=3.502481
PRIMER_LEFT_49_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_49_SEQUENCE=GGCGTTGGTGATCGTTATGG
PRIMER_LEFT_49=488,20
PRIMER_RIGHT_49=1333,20
PRIMER_LEFT_49_TM=59.673
PRIMER_RIGHT_49_TM=63.502
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=846
=
SEQUENCE_ID=MAGIC.175_4_10841071_10841832_ws-0_wu-0_1258573_66237_TRUE
SEQUENCE_TARGET=950,861
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAACATGGAACAGTGACTATCAAAATTGAGTGAAGCTGTAAGATGATGTCGGAGACTTAGTCATATANTACTACGCGTAAAAAATTTNTTTATCTTATATTGGCGAGAGTTTACAAAACATANNAGAGTTATTTATGCTGTACNAGTGAAGCCGACAAAGAGAATGAATCAAAGAAACGGCCAAAAGCATCGTCAATTGAACCNTTTCTGCAGTCAAANNTTTTACTCGATTGCCTTTGTTTTCAATTGATCTAAAAATATTCATAAATTGAATTTCGTGGTCAAAGTTTATGACCAGTCCTATAGGTATATATCATAAAGTCAACTTCGNATCAATTATTGTTAAATTCTGTTCACATTTCNCTCACATGCTTAGAATCTGNGTTTGACTTTTAAAAACTTTTAATTNGAANTTTCAAAACGTATATTGTTCANTTGTTCATACGAATATATACAANTTNTTTTNACTAAAAATTTGGAATCATCTTTATTATTAACATTCACGTAACGAATTNGGTAATCNTCAAACAAGTAAGAAATTGTATATCATATCGNNAACTCCGNNNNNNANNAAAAAAAAAAAAANANAGANNNGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATAACGTCGNNNNNNNTGATCTTGTATGGAGGTAAATACCGATTAATTCTTAGTAACTGCCTGACCNGATCCACAAGTTGACCATGACGCANNTTTTTTTAGCNTCNTANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACATTATATAATATACATTTGTATGATGTAATGCAAAATAACCAAAAGCGTGTGGTCCAAAGNTTTGTTCNAAAGAAGGGTATGGAGTAAAGTAAATGATTAATCTTATGGATCCGTGTCAATTATCAAATTCCGTTATCCCCGAAAATTGTTATANTTTNCTTTTAAAACTATTTGAAATGATCTAATTAACACAAATATTAANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGATATTTAGTTTCAATATCAGNATAGATCTACTCTAGTTATGACAGCAAACTGTTGTGATTGCACTTGTTAAAATTAAGAAAAGATAAAAGCGACTCTTGAGCATCAAGTAAGGACAATTAACATCCTCGACAAGCAAAACAACGACCAAGTCAATATAATCATAGCTAAATTCTAATTGTATCACCTAACAAAACAAAAACGTTTNNTTGTATTTTTATGAGTTTTGAAAATTACGGCTATAGCGTGAAAGGTGAGACGTTTGTCTTTAGTCTTTACATGTCAATTTACGAAATGACGACTGAAATCAAATTATTCTACGTGTTTTGATGTGCATGGCGTTTAGCTTATTGTTTTTTTCGATATNCGATAATAGTTTTAAGTCTACCAGTTTTATATAATAAAATGGCTATTATANTTATATCTACNGTATATGNTATTTACACCAATTTTAGCAACATGCTTCTTGACTCCGACTATAAGTAATTGAGATGGACCCAAAACAATTNTAATAGTGAGGCCCATACAAGGCCCAAAGTGAACTGAATAACTAAGATACAGTTATCTCCGCGCCAACACAAAACGACGGTAAATTACTACTTGCCTGAGAAAATTTTGCCCCCTTTTGCTTCAATTTGTTGTTTCCTTAAACCCTTCAAAGCAAAATTAGGGTTTCTCTTGGTTTTCGCAAATTAGGGATTTTTTCTCTCGTTCTCATGAAAAGATGAGTTTGATTTCCAAATCCTTGGCAAGAATTGAATGTTCGCCATTTTTTTATCCAAGAGCTTCAGAAATCACTACCGGAAAACGGATAACCTCTCCGCAAATCCGTTTATACACTGCGGCGGCCGTCGGCGGGAAAACTGGAAATGGAGAAAGGAANCAAAGAGCCAAAGCACCGGCGAAAACTCCGGAGGCAGTGACACCTGTGAAACCGCTGGAGATTNCGTCAGTCACGGCGACGACGGAGAATGAATTGCCTCGGCCTAATGAGATAGCTTACGAGAGTGAAGTAGCGAATTGGGTTAATCTGATTGGATTTGTTGATCAACCAGTTCAATTCGAAGCTTCTTCTGATGGCAAATTCTGGGCTGGTACTGTTATTTCCCAGAGGTCTNCTTCAGATTCCTCTGGTTTCTGGTACTCTCTCTAATCTATNCTCAAANATGAGANTTTTATTTGTAACTTNNTTTGTGATTCATATTTGAAAAGGTTTTGGATTTTGTTTTGTCAGGATTCCGATAATATTTGAAGGCGATTTAGCTAAAACCGCAGCTCGATATNTAAGCAAAGACGATCAAATTCATGTGTCTGGGAAGCTGTTTATAGATTCTCCTCCTCCAAATATGACTTATGCACAAGCCAATGTTCAGGTGTGANCTTTAAATAGTATTTGATCAGTTTTATGATACTTGTGAGANNNNTACT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_10481413_10481422_tsu-0_ct-1_2047_1475368_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14945913_14946127_ws-0_zu-0_41842_4428_TRUE
SEQUENCE_TARGET=950,314
SEQUENCE_TEMPLATE=AGTCCAACAGTACCTAACATCACCTCCATACATTGTTTAAAACANTTAGAGATCCATCTTTGNATGTANCAGAGTGAAACTCAACNAATNTNTCNATNAANTTACCTTCCGGATAAAGAGATTTGGTGAATGAATCTCCATGGTGAAAACAAACTCTGGAGCAGCAGAGACAACATGAACTCGATGTCCAGANGATATCAGATNCCGAACCACCTAAAANCATTGCATGAATAAAGATAAATCTACGATATCAAAATGTNATGATCTCAGGGATTCTAACCTCGACAACACGAGTGGCATGACCAAAGCCATGGCCAGTAACGTAGTAAGCGAAAACCAAAGAAGAACGTNGTGAAGAGNNNNNNNNNNNNNNNGACATTGGAGCTGTTTGAAGCAGAGTGGGCATTACACAATGTTAAATCAACCAATCTTTACNTTTGATTCTAAAGAGNAAAAAAAATGNGTTTTGTTTTGTTGGTTAGCCATAAATGGGAGAAANCAGAAGATGATAAGATAAAAGCGAATATAGATATGAGTAAAGCGTTACATAAGCTAAATATGGAGTCTACCGATTTTTTNTAACAACTTNAATCTTCTCTGTCCCTTTTCTTACGTTGTTGTTGCAGAGGAAAGTTGTCATATTTTTTTTNGTTCTTCAACAAACTNAAAAAGCANTAGCTTCCAAATTCGTTTCTAACNATTTTTTTTCTTTGTGNCTCTTTAACTTTTTACCTGCAATTTCTTCNNNNNNNNNNATATATTATTTTCGCCATATGGACCATATGTAATTCCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGNAATGATCNAAAAGAAATATGGCGTANGGTAAATGTGAAAATNAATTTNATATATTATTGCANAAGTTANNGGAGAGAAATGTTAGAGAATTTCAAGGCNTATTTTTNTTGNTATTTTTAAGATTTGTTTTGCTATGTANATACAATGTTGACAGGGTTTTCNTAGTCAAAGCACGGTAAANTCTATTAACAANACGTTTGTCACAATTGGAATAATGGACACATTTCAACCCAACAAAATAATANAACAACCCATTATGATATGTTATATCAAAACGTCAGAAAACAAATCAAATGTAANGTAATTTCATGCAGNAACTTTAAACAGATCAGTCTTCTTTGATTCACAAGCTTTCTAAAAAAAAGGTAACAAAAGAAAGATTCATAAGATGGAAGATAAAGCTAGGTTTCGAAGTTAGGCACACCCATTGGCCATGGCAATGAAGTGGTTAACTATATTAAGAGAGACCCTAAGATTTTGTATAAGACAGATCAAANCTAGAAAGNCTGACCTAGTACTGCCTCTGTCTTTTGCCTGGAGTGTTTGTAGTTTGAGCAGGGGAAGTGCAATCTCTTGACCAATGTCCTGCTTTTCCGCATTTGTAGCAATCTCCAGTTGAGGATGTGGATTTTGCTTGCCCTGATTGAAATTGTTGATTACTAGATTGACCGGTGCAGTCTCTCGACCAATGTCCTTGCTTCCCGCATTTGTAACATTCTCCACTTGAAGATGACGACTTCATTTGCCCTGGTTCATACTTGGGATTACCAGATTGAGCAGTGCAATCTCTCNACCAGTGCCCTGGCTTTCCGCATTTAAAACAATCTCCAGCTGCAGATGTAGACTTTACTGGTCCCGTATCAGNCTGAACAGTGCAATCTCTTGCCCAGTGCCCTTCTTTCCCACATTTATAACAGGGCGTGCCCGTTTTCGCATTTTGATANCCTCTGGTATCTGAGTCACCAAAGTTGCTGTTTTTGGTAACACTATACGACGTAGGAGAGCTGACTACATTGTCATTGCACCATTTGAAGAAACCACAAGAGCCCTGAAATTAATAAAAAGCCATGTTATCACACATACACTCAAAGGTAAGCAATACTACATTAACNACTTAACNATCGTGCACAATATTGACTCGGAATTCAATTTTTCACTTATTCCAGTGATGCACAAANTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.314386
PRIMER_LEFT_0_PENALTY=0.119028
PRIMER_RIGHT_0_PENALTY=0.195358
PRIMER_LEFT_0_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_0_SEQUENCE=TCAAGAGATTGCACTTCCCC
PRIMER_LEFT_0=612,20
PRIMER_RIGHT_0=1610,20
PRIMER_LEFT_0_TM=59.881
PRIMER_RIGHT_0_TM=60.195
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=10.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=999
PRIMER_PAIR_1_PENALTY=0.678491
PRIMER_LEFT_1_PENALTY=0.660780
PRIMER_RIGHT_1_PENALTY=0.017711
PRIMER_LEFT_1_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_1_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_1=611,20
PRIMER_RIGHT_1=1573,20
PRIMER_LEFT_1_TM=59.339
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=963
PRIMER_PAIR_2_PENALTY=1.028764
PRIMER_LEFT_2_PENALTY=0.119028
PRIMER_RIGHT_2_PENALTY=0.909736
PRIMER_LEFT_2_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_2_SEQUENCE=AGGCAAAAGACAGAGGCAGT
PRIMER_LEFT_2=612,20
PRIMER_RIGHT_2=1569,20
PRIMER_LEFT_2_TM=59.881
PRIMER_RIGHT_2_TM=59.090
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=958
PRIMER_PAIR_3_PENALTY=1.112020
PRIMER_LEFT_3_PENALTY=0.119028
PRIMER_RIGHT_3_PENALTY=0.992992
PRIMER_LEFT_3_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_3_SEQUENCE=CACTCCAGGCAAAAGACAGA
PRIMER_LEFT_3=612,20
PRIMER_RIGHT_3=1575,20
PRIMER_LEFT_3_TM=59.881
PRIMER_RIGHT_3_TM=59.007
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=964
PRIMER_PAIR_4_PENALTY=1.154801
PRIMER_LEFT_4_PENALTY=0.119028
PRIMER_RIGHT_4_PENALTY=1.035773
PRIMER_LEFT_4_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_4_SEQUENCE=CACTCCAGGCAAAAGACAGAG
PRIMER_LEFT_4=612,20
PRIMER_RIGHT_4=1575,21
PRIMER_LEFT_4_TM=59.881
PRIMER_RIGHT_4_TM=60.036
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=964
PRIMER_PAIR_5_PENALTY=1.219997
PRIMER_LEFT_5_PENALTY=0.119028
PRIMER_RIGHT_5_PENALTY=1.100969
PRIMER_LEFT_5_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_5_SEQUENCE=ACACTCCAGGCAAAAGACAGA
PRIMER_LEFT_5=612,20
PRIMER_RIGHT_5=1576,21
PRIMER_LEFT_5_TM=59.881
PRIMER_RIGHT_5_TM=59.899
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=965
PRIMER_PAIR_6_PENALTY=1.221928
PRIMER_LEFT_6_PENALTY=0.119028
PRIMER_RIGHT_6_PENALTY=1.102899
PRIMER_LEFT_6_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_6_SEQUENCE=CAAACACTCCAGGCAAAAGA
PRIMER_LEFT_6=612,20
PRIMER_RIGHT_6=1579,20
PRIMER_LEFT_6_TM=59.881
PRIMER_RIGHT_6_TM=58.897
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=968
PRIMER_PAIR_7_PENALTY=1.223318
PRIMER_LEFT_7_PENALTY=0.119028
PRIMER_RIGHT_7_PENALTY=1.104290
PRIMER_LEFT_7_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_7_SEQUENCE=CAGGCAAAAGACAGAGGCAG
PRIMER_LEFT_7=612,20
PRIMER_RIGHT_7=1570,20
PRIMER_LEFT_7_TM=59.881
PRIMER_RIGHT_7_TM=61.104
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=959
PRIMER_PAIR_8_PENALTY=1.353371
PRIMER_LEFT_8_PENALTY=0.119028
PRIMER_RIGHT_8_PENALTY=1.234342
PRIMER_LEFT_8_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_8_SEQUENCE=AAACACTCCAGGCAAAAGACA
PRIMER_LEFT_8=612,20
PRIMER_RIGHT_8=1578,21
PRIMER_LEFT_8_TM=59.881
PRIMER_RIGHT_8_TM=59.766
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=967
PRIMER_PAIR_9_PENALTY=1.353371
PRIMER_LEFT_9_PENALTY=0.119028
PRIMER_RIGHT_9_PENALTY=1.234342
PRIMER_LEFT_9_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_9_SEQUENCE=CAAACACTCCAGGCAAAAGAC
PRIMER_LEFT_9=612,20
PRIMER_RIGHT_9=1579,21
PRIMER_LEFT_9_TM=59.881
PRIMER_RIGHT_9_TM=59.766
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=968
PRIMER_PAIR_10_PENALTY=1.353371
PRIMER_LEFT_10_PENALTY=0.119028
PRIMER_RIGHT_10_PENALTY=1.234342
PRIMER_LEFT_10_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_10_SEQUENCE=ACAAACACTCCAGGCAAAAGA
PRIMER_LEFT_10=612,20
PRIMER_RIGHT_10=1580,21
PRIMER_LEFT_10_TM=59.881
PRIMER_RIGHT_10_TM=59.766
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=969
PRIMER_PAIR_11_PENALTY=1.570516
PRIMER_LEFT_11_PENALTY=0.660780
PRIMER_RIGHT_11_PENALTY=0.909736
PRIMER_LEFT_11_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_11_SEQUENCE=AGGCAAAAGACAGAGGCAGT
PRIMER_LEFT_11=611,20
PRIMER_RIGHT_11=1569,20
PRIMER_LEFT_11_TM=59.339
PRIMER_RIGHT_11_TM=59.090
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=959
PRIMER_PAIR_12_PENALTY=1.641449
PRIMER_LEFT_12_PENALTY=1.623739
PRIMER_RIGHT_12_PENALTY=0.017711
PRIMER_LEFT_12_SEQUENCE=TACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_12_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_12=610,21
PRIMER_RIGHT_12=1573,20
PRIMER_LEFT_12_TM=59.376
PRIMER_RIGHT_12_TM=59.982
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=964
PRIMER_PAIR_13_PENALTY=1.641449
PRIMER_LEFT_13_PENALTY=1.623739
PRIMER_RIGHT_13_PENALTY=0.017711
PRIMER_LEFT_13_SEQUENCE=TTACGTTGTTGTTGCAGAGGA
PRIMER_RIGHT_13_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_13=609,21
PRIMER_RIGHT_13=1573,20
PRIMER_LEFT_13_TM=59.376
PRIMER_RIGHT_13_TM=59.982
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=965
PRIMER_PAIR_14_PENALTY=1.653772
PRIMER_LEFT_14_PENALTY=0.660780
PRIMER_RIGHT_14_PENALTY=0.992992
PRIMER_LEFT_14_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_14_SEQUENCE=CACTCCAGGCAAAAGACAGA
PRIMER_LEFT_14=611,20
PRIMER_RIGHT_14=1575,20
PRIMER_LEFT_14_TM=59.339
PRIMER_RIGHT_14_TM=59.007
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=965
PRIMER_PAIR_15_PENALTY=1.696553
PRIMER_LEFT_15_PENALTY=0.660780
PRIMER_RIGHT_15_PENALTY=1.035773
PRIMER_LEFT_15_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_15_SEQUENCE=CACTCCAGGCAAAAGACAGAG
PRIMER_LEFT_15=611,20
PRIMER_RIGHT_15=1575,21
PRIMER_LEFT_15_TM=59.339
PRIMER_RIGHT_15_TM=60.036
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=965
PRIMER_PAIR_16_PENALTY=1.732608
PRIMER_LEFT_16_PENALTY=0.119028
PRIMER_RIGHT_16_PENALTY=1.613579
PRIMER_LEFT_16_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_16_SEQUENCE=AACACTCCAGGCAAAAGACAG
PRIMER_LEFT_16=612,20
PRIMER_RIGHT_16=1577,21
PRIMER_LEFT_16_TM=59.881
PRIMER_RIGHT_16_TM=59.386
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=966
PRIMER_PAIR_17_PENALTY=1.761749
PRIMER_LEFT_17_PENALTY=0.660780
PRIMER_RIGHT_17_PENALTY=1.100969
PRIMER_LEFT_17_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_17_SEQUENCE=ACACTCCAGGCAAAAGACAGA
PRIMER_LEFT_17=611,20
PRIMER_RIGHT_17=1576,21
PRIMER_LEFT_17_TM=59.339
PRIMER_RIGHT_17_TM=59.899
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=966
PRIMER_PAIR_18_PENALTY=1.763680
PRIMER_LEFT_18_PENALTY=0.660780
PRIMER_RIGHT_18_PENALTY=1.102899
PRIMER_LEFT_18_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_18_SEQUENCE=CAAACACTCCAGGCAAAAGA
PRIMER_LEFT_18=611,20
PRIMER_RIGHT_18=1579,20
PRIMER_LEFT_18_TM=59.339
PRIMER_RIGHT_18_TM=58.897
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=969
PRIMER_PAIR_19_PENALTY=1.765070
PRIMER_LEFT_19_PENALTY=0.660780
PRIMER_RIGHT_19_PENALTY=1.104290
PRIMER_LEFT_19_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_19_SEQUENCE=CAGGCAAAAGACAGAGGCAG
PRIMER_LEFT_19=611,20
PRIMER_RIGHT_19=1570,20
PRIMER_LEFT_19_TM=59.339
PRIMER_RIGHT_19_TM=61.104
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=960
PRIMER_PAIR_20_PENALTY=1.776538
PRIMER_LEFT_20_PENALTY=0.119028
PRIMER_RIGHT_20_PENALTY=1.657509
PRIMER_LEFT_20_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_20_SEQUENCE=AACACTCCAGGCAAAAGACA
PRIMER_LEFT_20=612,20
PRIMER_RIGHT_20=1577,20
PRIMER_LEFT_20_TM=59.881
PRIMER_RIGHT_20_TM=58.342
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=966
PRIMER_PAIR_21_PENALTY=1.814369
PRIMER_LEFT_21_PENALTY=0.660780
PRIMER_RIGHT_21_PENALTY=1.153589
PRIMER_LEFT_21_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_21_SEQUENCE=CCCCTGCTCAAACTACAAACA
PRIMER_LEFT_21=611,20
PRIMER_RIGHT_21=1594,21
PRIMER_LEFT_21_TM=59.339
PRIMER_RIGHT_21_TM=60.154
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=7.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=984
PRIMER_PAIR_22_PENALTY=1.895122
PRIMER_LEFT_22_PENALTY=0.660780
PRIMER_RIGHT_22_PENALTY=1.234342
PRIMER_LEFT_22_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_22_SEQUENCE=AAACACTCCAGGCAAAAGACA
PRIMER_LEFT_22=611,20
PRIMER_RIGHT_22=1578,21
PRIMER_LEFT_22_TM=59.339
PRIMER_RIGHT_22_TM=59.766
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=968
PRIMER_PAIR_23_PENALTY=1.895122
PRIMER_LEFT_23_PENALTY=0.660780
PRIMER_RIGHT_23_PENALTY=1.234342
PRIMER_LEFT_23_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_23_SEQUENCE=CAAACACTCCAGGCAAAAGAC
PRIMER_LEFT_23=611,20
PRIMER_RIGHT_23=1579,21
PRIMER_LEFT_23_TM=59.339
PRIMER_RIGHT_23_TM=59.766
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=969
PRIMER_PAIR_24_PENALTY=1.895122
PRIMER_LEFT_24_PENALTY=0.660780
PRIMER_RIGHT_24_PENALTY=1.234342
PRIMER_LEFT_24_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_24_SEQUENCE=ACAAACACTCCAGGCAAAAGA
PRIMER_LEFT_24=611,20
PRIMER_RIGHT_24=1580,21
PRIMER_LEFT_24_TM=59.339
PRIMER_RIGHT_24_TM=59.766
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=970
PRIMER_PAIR_25_PENALTY=1.910544
PRIMER_LEFT_25_PENALTY=1.715186
PRIMER_RIGHT_25_PENALTY=0.195358
PRIMER_LEFT_25_SEQUENCE=ACGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_25_SEQUENCE=TCAAGAGATTGCACTTCCCC
PRIMER_LEFT_25=611,21
PRIMER_RIGHT_25=1610,20
PRIMER_LEFT_25_TM=60.715
PRIMER_RIGHT_25_TM=60.195
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=10.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=1000
PRIMER_PAIR_26_PENALTY=1.920663
PRIMER_LEFT_26_PENALTY=0.119028
PRIMER_RIGHT_26_PENALTY=1.801634
PRIMER_LEFT_26_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_26_SEQUENCE=ACTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_26=612,20
PRIMER_RIGHT_26=1574,21
PRIMER_LEFT_26_TM=59.881
PRIMER_RIGHT_26_TM=60.802
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=963
PRIMER_PAIR_27_PENALTY=2.017970
PRIMER_LEFT_27_PENALTY=0.119028
PRIMER_RIGHT_27_PENALTY=1.898942
PRIMER_LEFT_27_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_27_SEQUENCE=GGTGTGCCTAACTTCGAAACC
PRIMER_LEFT_27=612,20
PRIMER_RIGHT_27=1458,21
PRIMER_LEFT_27_TM=59.881
PRIMER_RIGHT_27_TM=60.899
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=847
PRIMER_PAIR_28_PENALTY=2.037273
PRIMER_LEFT_28_PENALTY=1.841915
PRIMER_RIGHT_28_PENALTY=0.195358
PRIMER_LEFT_28_SEQUENCE=CGTTGTTGTTGCAGAGGAAAG
PRIMER_RIGHT_28_SEQUENCE=TCAAGAGATTGCACTTCCCC
PRIMER_LEFT_28=612,21
PRIMER_RIGHT_28=1610,20
PRIMER_LEFT_28_TM=60.842
PRIMER_RIGHT_28_TM=60.195
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=10.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=999
PRIMER_PAIR_29_PENALTY=2.042780
PRIMER_LEFT_29_PENALTY=0.119028
PRIMER_RIGHT_29_PENALTY=1.923751
PRIMER_LEFT_29_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_29_SEQUENCE=CAATGGGTGTGCCTAACTTC
PRIMER_LEFT_29=612,20
PRIMER_RIGHT_29=1463,20
PRIMER_LEFT_29_TM=59.881
PRIMER_RIGHT_29_TM=58.076
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=852
PRIMER_PAIR_30_PENALTY=2.057518
PRIMER_LEFT_30_PENALTY=2.039807
PRIMER_RIGHT_30_PENALTY=0.017711
PRIMER_LEFT_30_SEQUENCE=TGTTGTTGCAGAGGAAAGTTG
PRIMER_RIGHT_30_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_30=615,21
PRIMER_RIGHT_30=1573,20
PRIMER_LEFT_30_TM=58.960
PRIMER_RIGHT_30_TM=59.982
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=959
PRIMER_PAIR_31_PENALTY=2.120536
PRIMER_LEFT_31_PENALTY=2.102825
PRIMER_RIGHT_31_PENALTY=0.017711
PRIMER_LEFT_31_SEQUENCE=TACGTTGTTGTTGCAGAGGA
PRIMER_RIGHT_31_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_31=610,20
PRIMER_RIGHT_31=1573,20
PRIMER_LEFT_31_TM=57.897
PRIMER_RIGHT_31_TM=59.982
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=964
PRIMER_PAIR_32_PENALTY=2.133174
PRIMER_LEFT_32_PENALTY=0.119028
PRIMER_RIGHT_32_PENALTY=2.014145
PRIMER_LEFT_32_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_32_SEQUENCE=GTCAAGAGATTGCACTTCCCC
PRIMER_LEFT_32=612,20
PRIMER_RIGHT_32=1611,21
PRIMER_LEFT_32_TM=59.881
PRIMER_RIGHT_32_TM=61.014
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=10.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=1000
PRIMER_PAIR_33_PENALTY=2.188875
PRIMER_LEFT_33_PENALTY=0.119028
PRIMER_RIGHT_33_PENALTY=2.069847
PRIMER_LEFT_33_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_33_SEQUENCE=ACACTCCAGGCAAAAGACAG
PRIMER_LEFT_33=612,20
PRIMER_RIGHT_33=1576,20
PRIMER_LEFT_33_TM=59.881
PRIMER_RIGHT_33_TM=57.930
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=965
PRIMER_PAIR_34_PENALTY=2.198475
PRIMER_LEFT_34_PENALTY=2.180765
PRIMER_RIGHT_34_PENALTY=0.017711
PRIMER_LEFT_34_SEQUENCE=TGTTGTTGCAGAGGAAAGTTGT
PRIMER_RIGHT_34_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_34=615,22
PRIMER_RIGHT_34=1573,20
PRIMER_LEFT_34_TM=59.819
PRIMER_RIGHT_34_TM=59.982
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=959
PRIMER_PAIR_35_PENALTY=2.235165
PRIMER_LEFT_35_PENALTY=2.039807
PRIMER_RIGHT_35_PENALTY=0.195358
PRIMER_LEFT_35_SEQUENCE=TGTTGTTGCAGAGGAAAGTTG
PRIMER_RIGHT_35_SEQUENCE=TCAAGAGATTGCACTTCCCC
PRIMER_LEFT_35=615,21
PRIMER_RIGHT_35=1610,20
PRIMER_LEFT_35_TM=58.960
PRIMER_RIGHT_35_TM=60.195
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=10.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=996
PRIMER_PAIR_36_PENALTY=2.263192
PRIMER_LEFT_36_PENALTY=0.119028
PRIMER_RIGHT_36_PENALTY=2.144164
PRIMER_LEFT_36_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_36_SEQUENCE=ACAAACACTCCAGGCAAAAG
PRIMER_LEFT_36=612,20
PRIMER_RIGHT_36=1580,20
PRIMER_LEFT_36_TM=59.881
PRIMER_RIGHT_36_TM=57.856
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=969
PRIMER_PAIR_37_PENALTY=2.274360
PRIMER_LEFT_37_PENALTY=0.660780
PRIMER_RIGHT_37_PENALTY=1.613579
PRIMER_LEFT_37_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_37_SEQUENCE=AACACTCCAGGCAAAAGACAG
PRIMER_LEFT_37=611,20
PRIMER_RIGHT_37=1577,21
PRIMER_LEFT_37_TM=59.339
PRIMER_RIGHT_37_TM=59.386
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=967
PRIMER_PAIR_38_PENALTY=2.318290
PRIMER_LEFT_38_PENALTY=0.660780
PRIMER_RIGHT_38_PENALTY=1.657509
PRIMER_LEFT_38_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_38_SEQUENCE=AACACTCCAGGCAAAAGACA
PRIMER_LEFT_38=611,20
PRIMER_RIGHT_38=1577,20
PRIMER_LEFT_38_TM=59.339
PRIMER_RIGHT_38_TM=58.342
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=967
PRIMER_PAIR_39_PENALTY=2.322371
PRIMER_LEFT_39_PENALTY=0.119028
PRIMER_RIGHT_39_PENALTY=2.203343
PRIMER_LEFT_39_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_39_SEQUENCE=CAAACTACAAACACTCCAGGCA
PRIMER_LEFT_39=612,20
PRIMER_RIGHT_39=1586,22
PRIMER_LEFT_39_TM=59.881
PRIMER_RIGHT_39_TM=60.203
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=975
PRIMER_PAIR_40_PENALTY=2.334244
PRIMER_LEFT_40_PENALTY=0.119028
PRIMER_RIGHT_40_PENALTY=2.215215
PRIMER_LEFT_40_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_40_SEQUENCE=TACAAACACTCCAGGCAAAAGA
PRIMER_LEFT_40=612,20
PRIMER_RIGHT_40=1581,22
PRIMER_LEFT_40_TM=59.881
PRIMER_RIGHT_40_TM=59.785
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=970
PRIMER_PAIR_41_PENALTY=2.335560
PRIMER_LEFT_41_PENALTY=2.317850
PRIMER_RIGHT_41_PENALTY=0.017711
PRIMER_LEFT_41_SEQUENCE=TTGTTGTTGCAGAGGAAAGTTG
PRIMER_RIGHT_41_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_41=614,22
PRIMER_RIGHT_41=1573,20
PRIMER_LEFT_41_TM=60.318
PRIMER_RIGHT_41_TM=59.982
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=960
PRIMER_PAIR_42_PENALTY=2.337694
PRIMER_LEFT_42_PENALTY=0.119028
PRIMER_RIGHT_42_PENALTY=2.218665
PRIMER_LEFT_42_SEQUENCE=CGTTGTTGTTGCAGAGGAAA
PRIMER_RIGHT_42_SEQUENCE=GCCAATGGGTGTGCCTAACT
PRIMER_LEFT_42=612,20
PRIMER_RIGHT_42=1465,20
PRIMER_LEFT_42_TM=59.881
PRIMER_RIGHT_42_TM=62.219
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=5.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=854
PRIMER_PAIR_43_PENALTY=2.356459
PRIMER_LEFT_43_PENALTY=2.338748
PRIMER_RIGHT_43_PENALTY=0.017711
PRIMER_LEFT_43_SEQUENCE=CTTACGTTGTTGTTGCAGAGGA
PRIMER_RIGHT_43_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_43=608,22
PRIMER_RIGHT_43=1573,20
PRIMER_LEFT_43_TM=60.339
PRIMER_RIGHT_43_TM=59.982
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=2.356459
PRIMER_LEFT_44_PENALTY=2.338748
PRIMER_RIGHT_44_PENALTY=0.017711
PRIMER_LEFT_44_SEQUENCE=TCTTACGTTGTTGTTGCAGAGG
PRIMER_RIGHT_44_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_44=607,22
PRIMER_RIGHT_44=1573,20
PRIMER_LEFT_44_TM=60.339
PRIMER_RIGHT_44_TM=59.982
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=967
PRIMER_PAIR_45_PENALTY=2.376122
PRIMER_LEFT_45_PENALTY=2.180765
PRIMER_RIGHT_45_PENALTY=0.195358
PRIMER_LEFT_45_SEQUENCE=TGTTGTTGCAGAGGAAAGTTGT
PRIMER_RIGHT_45_SEQUENCE=TCAAGAGATTGCACTTCCCC
PRIMER_LEFT_45=615,22
PRIMER_RIGHT_45=1610,20
PRIMER_LEFT_45_TM=59.819
PRIMER_RIGHT_45_TM=60.195
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=10.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=996
PRIMER_PAIR_46_PENALTY=2.462415
PRIMER_LEFT_46_PENALTY=0.660780
PRIMER_RIGHT_46_PENALTY=1.801634
PRIMER_LEFT_46_SEQUENCE=ACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_46_SEQUENCE=ACTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_46=611,20
PRIMER_RIGHT_46=1574,21
PRIMER_LEFT_46_TM=59.339
PRIMER_RIGHT_46_TM=60.802
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=964
PRIMER_PAIR_47_PENALTY=2.513208
PRIMER_LEFT_47_PENALTY=2.317850
PRIMER_RIGHT_47_PENALTY=0.195358
PRIMER_LEFT_47_SEQUENCE=TTGTTGTTGCAGAGGAAAGTTG
PRIMER_RIGHT_47_SEQUENCE=TCAAGAGATTGCACTTCCCC
PRIMER_LEFT_47=614,22
PRIMER_RIGHT_47=1610,20
PRIMER_LEFT_47_TM=60.318
PRIMER_RIGHT_47_TM=60.195
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=10.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=997
PRIMER_PAIR_48_PENALTY=2.521360
PRIMER_LEFT_48_PENALTY=2.503650
PRIMER_RIGHT_48_PENALTY=0.017711
PRIMER_LEFT_48_SEQUENCE=CTTACGTTGTTGTTGCAGAGG
PRIMER_RIGHT_48_SEQUENCE=CTCCAGGCAAAAGACAGAGG
PRIMER_LEFT_48=608,21
PRIMER_RIGHT_48=1573,20
PRIMER_LEFT_48_TM=58.496
PRIMER_RIGHT_48_TM=59.982
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=966
PRIMER_PAIR_49_PENALTY=2.533474
PRIMER_LEFT_49_PENALTY=1.623739
PRIMER_RIGHT_49_PENALTY=0.909736
PRIMER_LEFT_49_SEQUENCE=TACGTTGTTGTTGCAGAGGAA
PRIMER_RIGHT_49_SEQUENCE=AGGCAAAAGACAGAGGCAGT
PRIMER_LEFT_49=610,21
PRIMER_RIGHT_49=1569,20
PRIMER_LEFT_49_TM=59.376
PRIMER_RIGHT_49_TM=59.090
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=960
=
SEQUENCE_ID=MAGIC.105_4_2576672_2576758_po-0_can-0_87122_2922_TRUE
SEQUENCE_TARGET=950,186
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGTTGGTTTAGAACCAACTTGAAACCGTCTTGATCCNTTTGGTACAAGTTTAATTTGAAGGATTTTAAAAGAGATNAAANTTTTTGACNGCCGAAANTGTGGNNNGNAGGTCCTTTGGATATANANACANAATAAAANAATTNNNNNNNNNNNNNNNNGNGTTNGGACTCAAATAATATNTGGGTATGGTTTGGTATGGNNACAGGTANAGGCATNTGGGACAATTTGCACCACTGGGAGGGGANCAAACGGCNGCACANCTTCCAGGAGATTGGGTTTCNATTGGACACAGCAGCCAGTGGCTCTGGTANTCCGTCTACGCCAGGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATCCACNAANGAATNTTTTNANTTTNTACTNAANNTAGAAAATGTTTNGATNTGGAACAGTAAGCAAGTGAGCTGGCGTACGAGAGTGCTNGTNGTTTCAGATTGGATGAGGCGTTTCATCTTTGGTAGNGATTCCAGTAGCATCTGATTTTCAANCTCCAAGGCTNCAANACACNNNTCTCTNTNTNTCACGAGTTACACTAACNTTCNCATGANGAAAAAGNTTTGATTCACTNNNAAAANNNGAAATCCAAATCGGNTTTTATATTTTGTAATAAAATAATGACCACANGTAACGAGGAGAGANTNNCTCGTTTCGTTGGCCATATCGTTGCTGANCCNGAGGANTNTACTCTCTGNTTTTCTCTNCAAATATTNTTCAGGTTGGCTTTTTACCTTTATATCCTCNTTTTTTGTAAGCNNTTTCNTCAAATTTTGTTTAGTACGATGACTAAAACTGAGTTTTGTTATTTCTTGNTCTCTGTTTTTCTCCTTCAATATCATGGTCTNTTTNTNTNTTCAAAATATTGGTATTTTTNATATCGTGGATTAGCTNAAAATGGNTTTAANTNNTTNTNNANNAAAGANATGGAGTGANNAAAAANNTTTCANNNNNNTTNTTTATATTAAAGTGGCTAGGAAAACAGAGAAATTACATAATCAANNNTTNAAAAGTTGAATTTNAANAAGTNNATCTAGAATAAGACTTGTTTTTTTAAGTGTCGTCAACATCTGTGACGCTGATAATATNAGCGAAACCAACTTTCTTGCGAAGCCGGANAACTAAACATGCGGCNTCGACACCTTCTCCGATCACCACCACTTTGTCTTTCTCTTCTCCTTCTATTCCCACAAANGTCACTCCTGCTCCNTTATTNAAACATGAGAANATGAGATCATTCCATCAAGCGAATTAGAACGAANATAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATATCTTACCAGTNGTTTTGGCTCCNATCTTGAGNGCTTCTGAACGACATTTGTCGCACCTCATACTTANCGNCATCAAGATTTTCTGCNCANNAAANTTCCAATNTTNCAAATAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTACCTTGGCCANTTTGGAACCTCTTNGAGAAAATGAAACNTTNTTTTNNNNNTCCTTTTNGTATAANTGATNNNTNTAATTTACGTGTGTANATATANAAGTGNTNCTGTNNTTGTCTCATTTTNTTATGGCANATNGNTTAACNNNNNNNNNNANNCNTATTTNATNTGTACTATTANTTGCNANGAANTCGCANAGTAATTTTCATGNAACTGCTTCGTANTTAATATTAGTNNANATNTNNACGTTTAANTGGACAATATANGGTATTGCTTGACTCANAAGGGTTAGANTTGGTNAGGTAAAGGNTAGATTTTTNTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.104657
PRIMER_LEFT_0_PENALTY=0.073614
PRIMER_RIGHT_0_PENALTY=0.031043
PRIMER_LEFT_0_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_0_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_0=509,20
PRIMER_RIGHT_0=1222,20
PRIMER_LEFT_0_TM=60.074
PRIMER_RIGHT_0_TM=59.969
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=714
PRIMER_PAIR_1_PENALTY=0.185428
PRIMER_LEFT_1_PENALTY=0.073614
PRIMER_RIGHT_1_PENALTY=0.111814
PRIMER_LEFT_1_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_1_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_1=509,20
PRIMER_RIGHT_1=1226,20
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.112
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=718
PRIMER_PAIR_2_PENALTY=0.574838
PRIMER_LEFT_2_PENALTY=0.073614
PRIMER_RIGHT_2_PENALTY=0.501224
PRIMER_LEFT_2_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_2_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_2=509,20
PRIMER_RIGHT_2=1173,20
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.499
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=665
PRIMER_PAIR_3_PENALTY=0.583945
PRIMER_LEFT_3_PENALTY=0.073614
PRIMER_RIGHT_3_PENALTY=0.510331
PRIMER_LEFT_3_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_3_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_3=509,20
PRIMER_RIGHT_3=1227,20
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.510
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=719
PRIMER_PAIR_4_PENALTY=0.583945
PRIMER_LEFT_4_PENALTY=0.073614
PRIMER_RIGHT_4_PENALTY=0.510331
PRIMER_LEFT_4_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_4_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_4=509,20
PRIMER_RIGHT_4=1228,20
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.510
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=720
PRIMER_PAIR_5_PENALTY=0.634202
PRIMER_LEFT_5_PENALTY=0.603160
PRIMER_RIGHT_5_PENALTY=0.031043
PRIMER_LEFT_5_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_5_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_5=500,20
PRIMER_RIGHT_5=1222,20
PRIMER_LEFT_5_TM=60.603
PRIMER_RIGHT_5_TM=59.969
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.7000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=723
PRIMER_PAIR_6_PENALTY=0.634202
PRIMER_LEFT_6_PENALTY=0.603160
PRIMER_RIGHT_6_PENALTY=0.031043
PRIMER_LEFT_6_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_6_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_6=499,20
PRIMER_RIGHT_6=1222,20
PRIMER_LEFT_6_TM=60.603
PRIMER_RIGHT_6_TM=59.969
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=724
PRIMER_PAIR_7_PENALTY=0.667075
PRIMER_LEFT_7_PENALTY=0.636033
PRIMER_RIGHT_7_PENALTY=0.031043
PRIMER_LEFT_7_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_7_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_7=510,20
PRIMER_RIGHT_7=1222,20
PRIMER_LEFT_7_TM=60.636
PRIMER_RIGHT_7_TM=59.969
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=713
PRIMER_PAIR_8_PENALTY=0.714974
PRIMER_LEFT_8_PENALTY=0.603160
PRIMER_RIGHT_8_PENALTY=0.111814
PRIMER_LEFT_8_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_8_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_8=500,20
PRIMER_RIGHT_8=1226,20
PRIMER_LEFT_8_TM=60.603
PRIMER_RIGHT_8_TM=60.112
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=727
PRIMER_PAIR_9_PENALTY=0.714974
PRIMER_LEFT_9_PENALTY=0.603160
PRIMER_RIGHT_9_PENALTY=0.111814
PRIMER_LEFT_9_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_9_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_9=499,20
PRIMER_RIGHT_9=1226,20
PRIMER_LEFT_9_TM=60.603
PRIMER_RIGHT_9_TM=60.112
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=728
PRIMER_PAIR_10_PENALTY=0.747846
PRIMER_LEFT_10_PENALTY=0.636033
PRIMER_RIGHT_10_PENALTY=0.111814
PRIMER_LEFT_10_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_10_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_10=510,20
PRIMER_RIGHT_10=1226,20
PRIMER_LEFT_10_TM=60.636
PRIMER_RIGHT_10_TM=60.112
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=717
PRIMER_PAIR_11_PENALTY=0.985440
PRIMER_LEFT_11_PENALTY=0.954397
PRIMER_RIGHT_11_PENALTY=0.031043
PRIMER_LEFT_11_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_11_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_11=260,20
PRIMER_RIGHT_11=1222,20
PRIMER_LEFT_11_TM=60.954
PRIMER_RIGHT_11_TM=59.969
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=963
PRIMER_PAIR_12_PENALTY=1.066211
PRIMER_LEFT_12_PENALTY=0.954397
PRIMER_RIGHT_12_PENALTY=0.111814
PRIMER_LEFT_12_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_12_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_12=260,20
PRIMER_RIGHT_12=1226,20
PRIMER_LEFT_12_TM=60.954
PRIMER_RIGHT_12_TM=60.112
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=967
PRIMER_PAIR_13_PENALTY=1.104384
PRIMER_LEFT_13_PENALTY=0.603160
PRIMER_RIGHT_13_PENALTY=0.501224
PRIMER_LEFT_13_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_13_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_13=500,20
PRIMER_RIGHT_13=1173,20
PRIMER_LEFT_13_TM=60.603
PRIMER_RIGHT_13_TM=59.499
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.7000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=674
PRIMER_PAIR_14_PENALTY=1.104384
PRIMER_LEFT_14_PENALTY=0.603160
PRIMER_RIGHT_14_PENALTY=0.501224
PRIMER_LEFT_14_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_14_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_14=499,20
PRIMER_RIGHT_14=1173,20
PRIMER_LEFT_14_TM=60.603
PRIMER_RIGHT_14_TM=59.499
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=675
PRIMER_PAIR_15_PENALTY=1.113491
PRIMER_LEFT_15_PENALTY=0.603160
PRIMER_RIGHT_15_PENALTY=0.510331
PRIMER_LEFT_15_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_15_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_15=500,20
PRIMER_RIGHT_15=1227,20
PRIMER_LEFT_15_TM=60.603
PRIMER_RIGHT_15_TM=60.510
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=728
PRIMER_PAIR_16_PENALTY=1.113491
PRIMER_LEFT_16_PENALTY=0.603160
PRIMER_RIGHT_16_PENALTY=0.510331
PRIMER_LEFT_16_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_16_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_16=500,20
PRIMER_RIGHT_16=1228,20
PRIMER_LEFT_16_TM=60.603
PRIMER_RIGHT_16_TM=60.510
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=729
PRIMER_PAIR_17_PENALTY=1.113491
PRIMER_LEFT_17_PENALTY=0.603160
PRIMER_RIGHT_17_PENALTY=0.510331
PRIMER_LEFT_17_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_17_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_17=499,20
PRIMER_RIGHT_17=1227,20
PRIMER_LEFT_17_TM=60.603
PRIMER_RIGHT_17_TM=60.510
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=729
PRIMER_PAIR_18_PENALTY=1.113491
PRIMER_LEFT_18_PENALTY=0.603160
PRIMER_RIGHT_18_PENALTY=0.510331
PRIMER_LEFT_18_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_18_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_18=499,20
PRIMER_RIGHT_18=1228,20
PRIMER_LEFT_18_TM=60.603
PRIMER_RIGHT_18_TM=60.510
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=730
PRIMER_PAIR_19_PENALTY=1.137257
PRIMER_LEFT_19_PENALTY=0.636033
PRIMER_RIGHT_19_PENALTY=0.501224
PRIMER_LEFT_19_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_19_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_19=510,20
PRIMER_RIGHT_19=1173,20
PRIMER_LEFT_19_TM=60.636
PRIMER_RIGHT_19_TM=59.499
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=664
PRIMER_PAIR_20_PENALTY=1.146364
PRIMER_LEFT_20_PENALTY=0.636033
PRIMER_RIGHT_20_PENALTY=0.510331
PRIMER_LEFT_20_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_20_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_20=510,20
PRIMER_RIGHT_20=1227,20
PRIMER_LEFT_20_TM=60.636
PRIMER_RIGHT_20_TM=60.510
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=718
PRIMER_PAIR_21_PENALTY=1.146364
PRIMER_LEFT_21_PENALTY=0.636033
PRIMER_RIGHT_21_PENALTY=0.510331
PRIMER_LEFT_21_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_21_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_21=510,20
PRIMER_RIGHT_21=1228,20
PRIMER_LEFT_21_TM=60.636
PRIMER_RIGHT_21_TM=60.510
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=719
PRIMER_PAIR_22_PENALTY=1.156363
PRIMER_LEFT_22_PENALTY=0.073614
PRIMER_RIGHT_22_PENALTY=1.082750
PRIMER_LEFT_22_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_22_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_22=509,20
PRIMER_RIGHT_22=1221,20
PRIMER_LEFT_22_TM=60.074
PRIMER_RIGHT_22_TM=61.083
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.8000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=713
PRIMER_PAIR_23_PENALTY=1.289680
PRIMER_LEFT_23_PENALTY=0.073614
PRIMER_RIGHT_23_PENALTY=1.216066
PRIMER_LEFT_23_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_23_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_23=509,20
PRIMER_RIGHT_23=1323,21
PRIMER_LEFT_23_TM=60.074
PRIMER_RIGHT_23_TM=60.216
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.8000
PRIMER_RIGHT_23_END_STABILITY=8.1000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=815
PRIMER_PAIR_24_PENALTY=1.289680
PRIMER_LEFT_24_PENALTY=0.073614
PRIMER_RIGHT_24_PENALTY=1.216066
PRIMER_LEFT_24_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_24_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_24=509,20
PRIMER_RIGHT_24=1324,21
PRIMER_LEFT_24_TM=60.074
PRIMER_RIGHT_24_TM=60.216
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=816
PRIMER_PAIR_25_PENALTY=1.455622
PRIMER_LEFT_25_PENALTY=0.954397
PRIMER_RIGHT_25_PENALTY=0.501224
PRIMER_LEFT_25_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_25_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_25=260,20
PRIMER_RIGHT_25=1173,20
PRIMER_LEFT_25_TM=60.954
PRIMER_RIGHT_25_TM=59.499
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=914
PRIMER_PAIR_26_PENALTY=1.456661
PRIMER_LEFT_26_PENALTY=0.073614
PRIMER_RIGHT_26_PENALTY=1.383047
PRIMER_LEFT_26_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_26_SEQUENCE=GACAAAGTGGTGGTGATCGG
PRIMER_LEFT_26=509,20
PRIMER_RIGHT_26=1231,20
PRIMER_LEFT_26_TM=60.074
PRIMER_RIGHT_26_TM=61.383
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.8000
PRIMER_RIGHT_26_END_STABILITY=9.8000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=723
PRIMER_PAIR_27_PENALTY=1.463306
PRIMER_LEFT_27_PENALTY=1.432264
PRIMER_RIGHT_27_PENALTY=0.031043
PRIMER_LEFT_27_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_27_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_27=300,20
PRIMER_RIGHT_27=1222,20
PRIMER_LEFT_27_TM=58.568
PRIMER_RIGHT_27_TM=59.969
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=923
PRIMER_PAIR_28_PENALTY=1.464728
PRIMER_LEFT_28_PENALTY=0.954397
PRIMER_RIGHT_28_PENALTY=0.510331
PRIMER_LEFT_28_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_28_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_28=260,20
PRIMER_RIGHT_28=1227,20
PRIMER_LEFT_28_TM=60.954
PRIMER_RIGHT_28_TM=60.510
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=968
PRIMER_PAIR_29_PENALTY=1.464728
PRIMER_LEFT_29_PENALTY=0.954397
PRIMER_RIGHT_29_PENALTY=0.510331
PRIMER_LEFT_29_SEQUENCE=ACAATTTGCACCACTGGGAG
PRIMER_RIGHT_29_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_29=260,20
PRIMER_RIGHT_29=1228,20
PRIMER_LEFT_29_TM=60.954
PRIMER_RIGHT_29_TM=60.510
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=7.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=969
PRIMER_PAIR_30_PENALTY=1.544077
PRIMER_LEFT_30_PENALTY=1.432264
PRIMER_RIGHT_30_PENALTY=0.111814
PRIMER_LEFT_30_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_30_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_30=300,20
PRIMER_RIGHT_30=1226,20
PRIMER_LEFT_30_TM=58.568
PRIMER_RIGHT_30_TM=60.112
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=927
PRIMER_PAIR_31_PENALTY=1.586605
PRIMER_LEFT_31_PENALTY=0.073614
PRIMER_RIGHT_31_PENALTY=1.512991
PRIMER_LEFT_31_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_31_SEQUENCE=CAAAGTGGTGGTGATCGGAG
PRIMER_LEFT_31=509,20
PRIMER_RIGHT_31=1229,20
PRIMER_LEFT_31_TM=60.074
PRIMER_RIGHT_31_TM=61.513
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.8000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=721
PRIMER_PAIR_32_PENALTY=1.685909
PRIMER_LEFT_32_PENALTY=0.603160
PRIMER_RIGHT_32_PENALTY=1.082750
PRIMER_LEFT_32_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_32_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_32=500,20
PRIMER_RIGHT_32=1221,20
PRIMER_LEFT_32_TM=60.603
PRIMER_RIGHT_32_TM=61.083
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.7000
PRIMER_RIGHT_32_END_STABILITY=6.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=722
PRIMER_PAIR_33_PENALTY=1.685909
PRIMER_LEFT_33_PENALTY=0.603160
PRIMER_RIGHT_33_PENALTY=1.082750
PRIMER_LEFT_33_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_33_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_33=499,20
PRIMER_RIGHT_33=1221,20
PRIMER_LEFT_33_TM=60.603
PRIMER_RIGHT_33_TM=61.083
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.9000
PRIMER_RIGHT_33_END_STABILITY=6.1000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=723
PRIMER_PAIR_34_PENALTY=1.686595
PRIMER_LEFT_34_PENALTY=0.073614
PRIMER_RIGHT_34_PENALTY=1.612981
PRIMER_LEFT_34_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_34_SEQUENCE=CGTTCTAATTCGCTTGATGG
PRIMER_LEFT_34=509,20
PRIMER_RIGHT_34=1323,20
PRIMER_LEFT_34_TM=60.074
PRIMER_RIGHT_34_TM=58.387
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=8.1000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=815
PRIMER_PAIR_35_PENALTY=1.718782
PRIMER_LEFT_35_PENALTY=0.636033
PRIMER_RIGHT_35_PENALTY=1.082750
PRIMER_LEFT_35_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_35_SEQUENCE=TGGTGATCGGAGAAGGTGTC
PRIMER_LEFT_35=510,20
PRIMER_RIGHT_35=1221,20
PRIMER_LEFT_35_TM=60.636
PRIMER_RIGHT_35_TM=61.083
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=712
PRIMER_PAIR_36_PENALTY=1.742272
PRIMER_LEFT_36_PENALTY=0.073614
PRIMER_RIGHT_36_PENALTY=1.668658
PRIMER_LEFT_36_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_36_SEQUENCE=GGCTTCGCAAGAAAGTTGGT
PRIMER_LEFT_36=509,20
PRIMER_RIGHT_36=1179,20
PRIMER_LEFT_36_TM=60.074
PRIMER_RIGHT_36_TM=61.669
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.8000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=671
PRIMER_PAIR_37_PENALTY=1.799825
PRIMER_LEFT_37_PENALTY=1.768783
PRIMER_RIGHT_37_PENALTY=0.031043
PRIMER_LEFT_37_SEQUENCE=TTTCGTTGGCCATATCGTTG
PRIMER_RIGHT_37_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_37=717,20
PRIMER_RIGHT_37=1222,20
PRIMER_LEFT_37_TM=61.769
PRIMER_RIGHT_37_TM=59.969
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=506
PRIMER_PAIR_38_PENALTY=1.819226
PRIMER_LEFT_38_PENALTY=0.603160
PRIMER_RIGHT_38_PENALTY=1.216066
PRIMER_LEFT_38_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_38_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_38=500,20
PRIMER_RIGHT_38=1323,21
PRIMER_LEFT_38_TM=60.603
PRIMER_RIGHT_38_TM=60.216
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.7000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=824
PRIMER_PAIR_39_PENALTY=1.819226
PRIMER_LEFT_39_PENALTY=0.603160
PRIMER_RIGHT_39_PENALTY=1.216066
PRIMER_LEFT_39_SEQUENCE=TCAGATTGGATGAGGCGTTT
PRIMER_RIGHT_39_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_39=500,20
PRIMER_RIGHT_39=1324,21
PRIMER_LEFT_39_TM=60.603
PRIMER_RIGHT_39_TM=60.216
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.7000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=825
PRIMER_PAIR_40_PENALTY=1.819226
PRIMER_LEFT_40_PENALTY=0.603160
PRIMER_RIGHT_40_PENALTY=1.216066
PRIMER_LEFT_40_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_40_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_40=499,20
PRIMER_RIGHT_40=1323,21
PRIMER_LEFT_40_TM=60.603
PRIMER_RIGHT_40_TM=60.216
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=825
PRIMER_PAIR_41_PENALTY=1.819226
PRIMER_LEFT_41_PENALTY=0.603160
PRIMER_RIGHT_41_PENALTY=1.216066
PRIMER_LEFT_41_SEQUENCE=TTCAGATTGGATGAGGCGTT
PRIMER_RIGHT_41_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_41=499,20
PRIMER_RIGHT_41=1324,21
PRIMER_LEFT_41_TM=60.603
PRIMER_RIGHT_41_TM=60.216
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=826
PRIMER_PAIR_42_PENALTY=1.838051
PRIMER_LEFT_42_PENALTY=0.073614
PRIMER_RIGHT_42_PENALTY=1.764437
PRIMER_LEFT_42_SEQUENCE=ATGAGGCGTTTCATCTTTGG
PRIMER_RIGHT_42_SEQUENCE=GCTTCGCAAGAAAGTTGGTTT
PRIMER_LEFT_42=509,20
PRIMER_RIGHT_42=1178,21
PRIMER_LEFT_42_TM=60.074
PRIMER_RIGHT_42_TM=60.764
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.8000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=670
PRIMER_PAIR_43_PENALTY=1.852099
PRIMER_LEFT_43_PENALTY=0.636033
PRIMER_RIGHT_43_PENALTY=1.216066
PRIMER_LEFT_43_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_43_SEQUENCE=CGTTCTAATTCGCTTGATGGA
PRIMER_LEFT_43=510,20
PRIMER_RIGHT_43=1323,21
PRIMER_LEFT_43_TM=60.636
PRIMER_RIGHT_43_TM=60.216
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=8.1000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=814
PRIMER_PAIR_44_PENALTY=1.852099
PRIMER_LEFT_44_PENALTY=0.636033
PRIMER_RIGHT_44_PENALTY=1.216066
PRIMER_LEFT_44_SEQUENCE=TGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_44_SEQUENCE=TCGTTCTAATTCGCTTGATGG
PRIMER_LEFT_44=510,20
PRIMER_RIGHT_44=1324,21
PRIMER_LEFT_44_TM=60.636
PRIMER_RIGHT_44_TM=60.216
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=815
PRIMER_PAIR_45_PENALTY=1.880597
PRIMER_LEFT_45_PENALTY=1.768783
PRIMER_RIGHT_45_PENALTY=0.111814
PRIMER_LEFT_45_SEQUENCE=TTTCGTTGGCCATATCGTTG
PRIMER_RIGHT_45_SEQUENCE=AGTGGTGGTGATCGGAGAAG
PRIMER_LEFT_45=717,20
PRIMER_RIGHT_45=1226,20
PRIMER_LEFT_45_TM=61.769
PRIMER_RIGHT_45_TM=60.112
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.7000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=510
PRIMER_PAIR_46_PENALTY=1.901384
PRIMER_LEFT_46_PENALTY=1.870342
PRIMER_RIGHT_46_PENALTY=0.031043
PRIMER_LEFT_46_SEQUENCE=ATGAGGCGTTTCATCTTTGGT
PRIMER_RIGHT_46_SEQUENCE=GTGGTGATCGGAGAAGGTGT
PRIMER_LEFT_46=509,21
PRIMER_RIGHT_46=1222,20
PRIMER_LEFT_46_TM=60.870
PRIMER_RIGHT_46_TM=59.969
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=714
PRIMER_PAIR_47_PENALTY=1.933488
PRIMER_LEFT_47_PENALTY=1.432264
PRIMER_RIGHT_47_PENALTY=0.501224
PRIMER_LEFT_47_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_47_SEQUENCE=GCAAGAAAGTTGGTTTCGCT
PRIMER_LEFT_47=300,20
PRIMER_RIGHT_47=1173,20
PRIMER_LEFT_47_TM=58.568
PRIMER_RIGHT_47_TM=59.499
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=874
PRIMER_PAIR_48_PENALTY=1.942595
PRIMER_LEFT_48_PENALTY=1.432264
PRIMER_RIGHT_48_PENALTY=0.510331
PRIMER_LEFT_48_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_48_SEQUENCE=AAGTGGTGGTGATCGGAGAA
PRIMER_LEFT_48=300,20
PRIMER_RIGHT_48=1227,20
PRIMER_LEFT_48_TM=58.568
PRIMER_RIGHT_48_TM=60.510
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=928
PRIMER_PAIR_49_PENALTY=1.942595
PRIMER_LEFT_49_PENALTY=1.432264
PRIMER_RIGHT_49_PENALTY=0.510331
PRIMER_LEFT_49_SEQUENCE=CTTCCAGGAGATTGGGTTTC
PRIMER_RIGHT_49_SEQUENCE=AAAGTGGTGGTGATCGGAGA
PRIMER_LEFT_49=300,20
PRIMER_RIGHT_49=1228,20
PRIMER_LEFT_49_TM=58.568
PRIMER_RIGHT_49_TM=60.510
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=929
=
SEQUENCE_ID=MAGIC.446_4_1909303_1909539_wu-0_sf-2_282082_21045_TRUE
SEQUENCE_TARGET=950,336
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNTAATATNATTATCTATANNNANAAGAACTNATAATTAACTGANNAAAANAAAANATTGTTGTNTTNTACTNTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_16125525_16125752_mt-0_ct-1_93378_605577_TRUE
SEQUENCE_TARGET=950,327
SEQUENCE_TEMPLATE=ATCATGAAGATGGAACATTTCCACTTTGANCATTGGATGTTTTCTTTTGTCTGATGGGCGCCGATGGTGGATCCAAGATATCCGTTTTACATCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCGGGTTTNGNCCTAGTTATANATTAAAGATTGTTTTGNGTGTTATGTCGAAAAACAAGTTTTTGAATCTATAACAAACAAGACAGTGATAAAANGTTTCCAAAATATGAGTGAGCGTAANATACATNNTTAAGAATTGTGTTNAATTTTTTTTCGTTNTNNNNNNNNNNTGACNNNNNANTTTATTTNGATGTTATGTATTGCTATATTTTTTNTTTGGTTGTAAAATTTTGAAGATACTTATTTAAAACNCATCAAACAATTTTTNTATATNNGTNATTNNACAAATAAGTTTTTNTANATATNTTAGTTTNAAAAACAAACTAATATNATATTTAGANCNAAATCANNTTTTCNNANATATTTTANTCTTCANNNNNAATCGATNTTTAAACTATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNGATAAANTTATCTTNANGTTATTTCGGATATTTTAGTTTTCTTTAGACATATGCCGGATCGGATCATNTANANTANTGTCATTNTCTCCNTAACCGGATTANATNACCAAATTGATCCANTTTCATTTCTCCCAATATTTATNNNNNNNTNTTTNTTTTNATGTNNNNNNNNNNNNNANTATTTAAATAAATNATTACCACTGAATCGAAGAAGCNTTGCTTAGATATCATCGAACTTGCTCCAACTGCTCTNTCTNAGGATCTCNNNNNNACNCAGTTTCTTCCATCCATGGCGTCTCTTTCTNCTACCTCTCTCTCTCTCCCCAAGAANTCTCACCAACTCCATCCTTCATCTGGTAATAACNTCTTCNATCTCTCCAATCTTCGTNCTTTATAGATTNTCNATATCCTCCANTTTCGANCTNATNAGATTNGTACAGTCATGTTACGATTTCTAATTTAGCATCTCANANAANCCANTTTANNTGTNTATTGGACGATCTTNATTCCTTTATGTTGTTGGGTCCTTTCNTTTTACTTGTGCTGATTTGTCNAAACANTGCAAAATTANANCTTGAAGCTGTTTTGTTAGAAAACTGTTCAACTTGTTATGTATAAGTCACTGATTNTTGNTTGTTCTTGTTACCTTCTAGGTTTTTCTCTGAATCCAAATGCTCGTTGTGTCAGTGTTTCATTTGGACTGAATCACTCCAACAAACTTCATATTTCTGCTCCTAGAACCAAAAGGATCCTAACCATTCAATCTGCATACAGGTATATAACTTTATCTTATACAAATTATTGTTTGAGATGTCGAAAACTGTGGTTNTTGTTACTCCTTAATGTTTTGTGAGCATNGTAACATTTTTTAGTATACTTTAGTTTTCTATTNGCAACTTNTGTTTACAGAGACAGTTTGCAATGTCTAATAGCATCAAAACTTGCAGAGATGATGATGGTTCAGGCAGCACAGGCCTATTCGTCGGAGGGTTTATTTTGGGCGGACTTATAGTCGGTGCCCTCGGATGTGTATATGCACCACAGNNNGTAATCTGTTACATGNTTTAGTTTGATCACTCTTAGAGCTTGTATTCGTTTNAATCAAGAAGTTCTTGAGTGGACTCGAGGAGCTCTGTAACCTTCTCTGTTTCACTTTGACTCATCAATGTGTGATGTTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.625986
PRIMER_LEFT_0_PENALTY=4.165121
PRIMER_RIGHT_0_PENALTY=0.460865
PRIMER_LEFT_0_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_0_SEQUENCE=GCCATGGATGGAAGAAACTG
PRIMER_LEFT_0=719,20
PRIMER_RIGHT_0=1342,20
PRIMER_LEFT_0_TM=55.835
PRIMER_RIGHT_0_TM=60.461
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=624
PRIMER_PAIR_1_PENALTY=4.725105
PRIMER_LEFT_1_PENALTY=4.165121
PRIMER_RIGHT_1_PENALTY=0.559985
PRIMER_LEFT_1_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_1_SEQUENCE=CAGTTGGAGCAAGTTCGATG
PRIMER_LEFT_1=719,20
PRIMER_RIGHT_1=1296,20
PRIMER_LEFT_1_TM=55.835
PRIMER_RIGHT_1_TM=59.440
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=578
PRIMER_PAIR_2_PENALTY=5.238475
PRIMER_LEFT_2_PENALTY=4.165121
PRIMER_RIGHT_2_PENALTY=1.073355
PRIMER_LEFT_2_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_2_SEQUENCE=TCTTGGGGAGAGAGAGAGAGG
PRIMER_LEFT_2=719,20
PRIMER_RIGHT_2=1377,21
PRIMER_LEFT_2_TM=55.835
PRIMER_RIGHT_2_TM=60.073
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=659
PRIMER_PAIR_3_PENALTY=5.246709
PRIMER_LEFT_3_PENALTY=4.165121
PRIMER_RIGHT_3_PENALTY=1.081589
PRIMER_LEFT_3_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_3_SEQUENCE=GATGAAGGATGGAGTTGGTGA
PRIMER_LEFT_3=719,20
PRIMER_RIGHT_3=1402,21
PRIMER_LEFT_3_TM=55.835
PRIMER_RIGHT_3_TM=59.918
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=684
PRIMER_PAIR_4_PENALTY=5.524072
PRIMER_LEFT_4_PENALTY=4.165121
PRIMER_RIGHT_4_PENALTY=1.358951
PRIMER_LEFT_4_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_4_SEQUENCE=TGAAGGATGGAGTTGGTGAG
PRIMER_LEFT_4=719,20
PRIMER_RIGHT_4=1400,20
PRIMER_LEFT_4_TM=55.835
PRIMER_RIGHT_4_TM=58.641
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=682
PRIMER_PAIR_5_PENALTY=5.805550
PRIMER_LEFT_5_PENALTY=4.165121
PRIMER_RIGHT_5_PENALTY=1.640429
PRIMER_LEFT_5_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_5_SEQUENCE=TGAAGGATGGAGTTGGTGAGA
PRIMER_LEFT_5=719,20
PRIMER_RIGHT_5=1400,21
PRIMER_LEFT_5_TM=55.835
PRIMER_RIGHT_5_TM=60.640
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=682
PRIMER_PAIR_6_PENALTY=6.034689
PRIMER_LEFT_6_PENALTY=4.165121
PRIMER_RIGHT_6_PENALTY=1.869568
PRIMER_LEFT_6_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_6_SEQUENCE=CTTGGGGAGAGAGAGAGAGG
PRIMER_LEFT_6=719,20
PRIMER_RIGHT_6=1376,20
PRIMER_LEFT_6_TM=55.835
PRIMER_RIGHT_6_TM=58.130
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=658
PRIMER_PAIR_7_PENALTY=6.093783
PRIMER_LEFT_7_PENALTY=4.165121
PRIMER_RIGHT_7_PENALTY=1.928663
PRIMER_LEFT_7_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_7_SEQUENCE=CAGATGAAGGATGGAGTTGG
PRIMER_LEFT_7=719,20
PRIMER_RIGHT_7=1404,20
PRIMER_LEFT_7_TM=55.835
PRIMER_RIGHT_7_TM=58.071
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=686
PRIMER_PAIR_8_PENALTY=6.093783
PRIMER_LEFT_8_PENALTY=4.165121
PRIMER_RIGHT_8_PENALTY=1.928663
PRIMER_LEFT_8_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_8_SEQUENCE=CCAGATGAAGGATGGAGTTG
PRIMER_LEFT_8=719,20
PRIMER_RIGHT_8=1405,20
PRIMER_LEFT_8_TM=55.835
PRIMER_RIGHT_8_TM=58.071
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=687
PRIMER_PAIR_9_PENALTY=6.124379
PRIMER_LEFT_9_PENALTY=4.165121
PRIMER_RIGHT_9_PENALTY=1.959258
PRIMER_LEFT_9_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_9_SEQUENCE=CTTGGGGAGAGAGAGAGAGGT
PRIMER_LEFT_9=719,20
PRIMER_RIGHT_9=1376,21
PRIMER_LEFT_9_TM=55.835
PRIMER_RIGHT_9_TM=59.041
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=57.143
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=658
PRIMER_PAIR_10_PENALTY=6.161799
PRIMER_LEFT_10_PENALTY=4.165121
PRIMER_RIGHT_10_PENALTY=1.996678
PRIMER_LEFT_10_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_10_SEQUENCE=ATGAAGGATGGAGTTGGTGAG
PRIMER_LEFT_10=719,20
PRIMER_RIGHT_10=1401,21
PRIMER_LEFT_10_TM=55.835
PRIMER_RIGHT_10_TM=59.003
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=683
PRIMER_PAIR_11_PENALTY=6.161799
PRIMER_LEFT_11_PENALTY=4.165121
PRIMER_RIGHT_11_PENALTY=1.996678
PRIMER_LEFT_11_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_11_SEQUENCE=AGATGAAGGATGGAGTTGGTG
PRIMER_LEFT_11=719,20
PRIMER_RIGHT_11=1403,21
PRIMER_LEFT_11_TM=55.835
PRIMER_RIGHT_11_TM=59.003
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.6000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=685
PRIMER_PAIR_12_PENALTY=6.161799
PRIMER_LEFT_12_PENALTY=4.165121
PRIMER_RIGHT_12_PENALTY=1.996678
PRIMER_LEFT_12_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_12_SEQUENCE=CAGATGAAGGATGGAGTTGGT
PRIMER_LEFT_12=719,20
PRIMER_RIGHT_12=1404,21
PRIMER_LEFT_12_TM=55.835
PRIMER_RIGHT_12_TM=59.003
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=686
PRIMER_PAIR_13_PENALTY=6.161799
PRIMER_LEFT_13_PENALTY=4.165121
PRIMER_RIGHT_13_PENALTY=1.996678
PRIMER_LEFT_13_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_13_SEQUENCE=ACCAGATGAAGGATGGAGTTG
PRIMER_LEFT_13=719,20
PRIMER_RIGHT_13=1406,21
PRIMER_LEFT_13_TM=55.835
PRIMER_RIGHT_13_TM=59.003
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=688
PRIMER_PAIR_14_PENALTY=6.191765
PRIMER_LEFT_14_PENALTY=4.165121
PRIMER_RIGHT_14_PENALTY=2.026644
PRIMER_LEFT_14_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_14_SEQUENCE=TTGGGGAGAGAGAGAGAGGT
PRIMER_LEFT_14=719,20
PRIMER_RIGHT_14=1375,20
PRIMER_LEFT_14_TM=55.835
PRIMER_RIGHT_14_TM=57.973
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=1.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=657
PRIMER_PAIR_15_PENALTY=6.254867
PRIMER_LEFT_15_PENALTY=4.165121
PRIMER_RIGHT_15_PENALTY=2.089746
PRIMER_LEFT_15_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_15_SEQUENCE=ATGAAGGATGGAGTTGGTGA
PRIMER_LEFT_15=719,20
PRIMER_RIGHT_15=1401,20
PRIMER_LEFT_15_TM=55.835
PRIMER_RIGHT_15_TM=57.910
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=683
PRIMER_PAIR_16_PENALTY=6.254867
PRIMER_LEFT_16_PENALTY=4.165121
PRIMER_RIGHT_16_PENALTY=2.089746
PRIMER_LEFT_16_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_16_SEQUENCE=GATGAAGGATGGAGTTGGTG
PRIMER_LEFT_16=719,20
PRIMER_RIGHT_16=1402,20
PRIMER_LEFT_16_TM=55.835
PRIMER_RIGHT_16_TM=57.910
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=684
PRIMER_PAIR_17_PENALTY=6.348962
PRIMER_LEFT_17_PENALTY=4.165121
PRIMER_RIGHT_17_PENALTY=2.183842
PRIMER_LEFT_17_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_17_SEQUENCE=ACGCCATGGATGGAAGAAAC
PRIMER_LEFT_17=719,20
PRIMER_RIGHT_17=1344,20
PRIMER_LEFT_17_TM=55.835
PRIMER_RIGHT_17_TM=62.184
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=626
PRIMER_PAIR_18_PENALTY=6.366770
PRIMER_LEFT_18_PENALTY=4.165121
PRIMER_RIGHT_18_PENALTY=2.201650
PRIMER_LEFT_18_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_18_SEQUENCE=GGACCCAACAACATAAAGGAA
PRIMER_LEFT_18=719,20
PRIMER_RIGHT_18=1575,21
PRIMER_LEFT_18_TM=55.835
PRIMER_RIGHT_18_TM=58.798
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.6000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=857
PRIMER_PAIR_19_PENALTY=6.424029
PRIMER_LEFT_19_PENALTY=4.165121
PRIMER_RIGHT_19_PENALTY=2.258908
PRIMER_LEFT_19_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_19_SEQUENCE=AGGACCCAACAACATAAAGGAA
PRIMER_LEFT_19=719,20
PRIMER_RIGHT_19=1576,22
PRIMER_LEFT_19_TM=55.835
PRIMER_RIGHT_19_TM=59.741
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=858
PRIMER_PAIR_20_PENALTY=6.424029
PRIMER_LEFT_20_PENALTY=4.165121
PRIMER_RIGHT_20_PENALTY=2.258908
PRIMER_LEFT_20_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_20_SEQUENCE=AAGGACCCAACAACATAAAGGA
PRIMER_LEFT_20=719,20
PRIMER_RIGHT_20=1577,22
PRIMER_LEFT_20_TM=55.835
PRIMER_RIGHT_20_TM=59.741
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.6000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=859
PRIMER_PAIR_21_PENALTY=6.464505
PRIMER_LEFT_21_PENALTY=4.165121
PRIMER_RIGHT_21_PENALTY=2.299385
PRIMER_LEFT_21_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_21_SEQUENCE=CGCCATGGATGGAAGAAACT
PRIMER_LEFT_21=719,20
PRIMER_RIGHT_21=1343,20
PRIMER_LEFT_21_TM=55.835
PRIMER_RIGHT_21_TM=62.299
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=625
PRIMER_PAIR_22_PENALTY=6.549390
PRIMER_LEFT_22_PENALTY=4.165121
PRIMER_RIGHT_22_PENALTY=2.384269
PRIMER_LEFT_22_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_22_SEQUENCE=GAAGGATGGAGTTGGTGAGA
PRIMER_LEFT_22=719,20
PRIMER_RIGHT_22=1399,20
PRIMER_LEFT_22_TM=55.835
PRIMER_RIGHT_22_TM=57.616
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=681
PRIMER_PAIR_23_PENALTY=6.742093
PRIMER_LEFT_23_PENALTY=4.165121
PRIMER_RIGHT_23_PENALTY=2.576973
PRIMER_LEFT_23_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_23_SEQUENCE=AGGACCCAACAACATAAAGGA
PRIMER_LEFT_23=719,20
PRIMER_RIGHT_23=1576,21
PRIMER_LEFT_23_TM=55.835
PRIMER_RIGHT_23_TM=58.423
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.6000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=858
PRIMER_PAIR_24_PENALTY=6.801602
PRIMER_LEFT_24_PENALTY=4.165121
PRIMER_RIGHT_24_PENALTY=2.636481
PRIMER_LEFT_24_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_24_SEQUENCE=GGACCCAACAACATAAAGGA
PRIMER_LEFT_24=719,20
PRIMER_RIGHT_24=1575,20
PRIMER_LEFT_24_TM=55.835
PRIMER_RIGHT_24_TM=57.364
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.6000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=857
PRIMER_PAIR_25_PENALTY=6.883101
PRIMER_LEFT_25_PENALTY=4.165121
PRIMER_RIGHT_25_PENALTY=2.717980
PRIMER_LEFT_25_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_25_SEQUENCE=AAAGGACCCAACAACATAAAGG
PRIMER_LEFT_25=719,20
PRIMER_RIGHT_25=1578,22
PRIMER_LEFT_25_TM=55.835
PRIMER_RIGHT_25_TM=59.282
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=860
PRIMER_PAIR_26_PENALTY=6.953797
PRIMER_LEFT_26_PENALTY=4.165121
PRIMER_RIGHT_26_PENALTY=2.788676
PRIMER_LEFT_26_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_26_SEQUENCE=CCAGATGAAGGATGGAGTTGG
PRIMER_LEFT_26=719,20
PRIMER_RIGHT_26=1405,21
PRIMER_LEFT_26_TM=55.835
PRIMER_RIGHT_26_TM=61.789
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=687
PRIMER_PAIR_27_PENALTY=7.045359
PRIMER_LEFT_27_PENALTY=4.165121
PRIMER_RIGHT_27_PENALTY=2.880239
PRIMER_LEFT_27_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_27_SEQUENCE=AGACGCCATGGATGGAAGAA
PRIMER_LEFT_27=719,20
PRIMER_RIGHT_27=1346,20
PRIMER_LEFT_27_TM=55.835
PRIMER_RIGHT_27_TM=62.880
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.6000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=628
PRIMER_PAIR_28_PENALTY=7.046248
PRIMER_LEFT_28_PENALTY=4.165121
PRIMER_RIGHT_28_PENALTY=2.881127
PRIMER_LEFT_28_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_28_SEQUENCE=TCTTGGGGAGAGAGAGAGAGGT
PRIMER_LEFT_28=719,20
PRIMER_RIGHT_28=1377,22
PRIMER_LEFT_28_TM=55.835
PRIMER_RIGHT_28_TM=60.881
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=54.545
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.6000
PRIMER_RIGHT_28_END_STABILITY=7.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=659
PRIMER_PAIR_29_PENALTY=7.047738
PRIMER_LEFT_29_PENALTY=4.165121
PRIMER_RIGHT_29_PENALTY=2.882618
PRIMER_LEFT_29_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_29_SEQUENCE=ATGAAGGATGGAGTTGGTGAGA
PRIMER_LEFT_29=719,20
PRIMER_RIGHT_29=1401,22
PRIMER_LEFT_29_TM=55.835
PRIMER_RIGHT_29_TM=60.883
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.6000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=683
PRIMER_PAIR_30_PENALTY=7.047738
PRIMER_LEFT_30_PENALTY=4.165121
PRIMER_RIGHT_30_PENALTY=2.882618
PRIMER_LEFT_30_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_30_SEQUENCE=GATGAAGGATGGAGTTGGTGAG
PRIMER_LEFT_30=719,20
PRIMER_RIGHT_30=1402,22
PRIMER_LEFT_30_TM=55.835
PRIMER_RIGHT_30_TM=60.883
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=684
PRIMER_PAIR_31_PENALTY=7.047738
PRIMER_LEFT_31_PENALTY=4.165121
PRIMER_RIGHT_31_PENALTY=2.882618
PRIMER_LEFT_31_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_31_SEQUENCE=AGATGAAGGATGGAGTTGGTGA
PRIMER_LEFT_31=719,20
PRIMER_RIGHT_31=1403,22
PRIMER_LEFT_31_TM=55.835
PRIMER_RIGHT_31_TM=60.883
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=685
PRIMER_PAIR_32_PENALTY=7.077854
PRIMER_LEFT_32_PENALTY=4.165121
PRIMER_RIGHT_32_PENALTY=2.912733
PRIMER_LEFT_32_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_32_SEQUENCE=TTGGGGAGAGAGAGAGAGGTAG
PRIMER_LEFT_32=719,20
PRIMER_RIGHT_32=1375,22
PRIMER_LEFT_32_TM=55.835
PRIMER_RIGHT_32_TM=59.087
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=54.545
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.6000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=657
PRIMER_PAIR_33_PENALTY=7.112896
PRIMER_LEFT_33_PENALTY=4.165121
PRIMER_RIGHT_33_PENALTY=2.947776
PRIMER_LEFT_33_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_33_SEQUENCE=TACCAGATGAAGGATGGAGTTG
PRIMER_LEFT_33=719,20
PRIMER_RIGHT_33=1407,22
PRIMER_LEFT_33_TM=55.835
PRIMER_RIGHT_33_TM=59.052
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.6000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=689
PRIMER_PAIR_34_PENALTY=7.185390
PRIMER_LEFT_34_PENALTY=4.165121
PRIMER_RIGHT_34_PENALTY=3.020269
PRIMER_LEFT_34_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_34_SEQUENCE=CTTGGGGAGAGAGAGAGAGGTAG
PRIMER_LEFT_34=719,20
PRIMER_RIGHT_34=1376,23
PRIMER_LEFT_34_TM=55.835
PRIMER_RIGHT_34_TM=60.020
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=56.522
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.6000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=658
PRIMER_PAIR_35_PENALTY=7.195029
PRIMER_LEFT_35_PENALTY=4.165121
PRIMER_RIGHT_35_PENALTY=3.029908
PRIMER_LEFT_35_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_35_SEQUENCE=AAGGACCCAACAACATAAAGG
PRIMER_LEFT_35=719,20
PRIMER_RIGHT_35=1577,21
PRIMER_LEFT_35_TM=55.835
PRIMER_RIGHT_35_TM=57.970
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.6000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=859
PRIMER_PAIR_36_PENALTY=7.376856
PRIMER_LEFT_36_PENALTY=4.165121
PRIMER_RIGHT_36_PENALTY=3.211736
PRIMER_LEFT_36_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_36_SEQUENCE=TTCTTGGGGAGAGAGAGAGAG
PRIMER_LEFT_36=719,20
PRIMER_RIGHT_36=1378,21
PRIMER_LEFT_36_TM=55.835
PRIMER_RIGHT_36_TM=57.788
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.6000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=660
PRIMER_PAIR_37_PENALTY=7.405645
PRIMER_LEFT_37_PENALTY=4.165121
PRIMER_RIGHT_37_PENALTY=3.240525
PRIMER_LEFT_37_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_37_SEQUENCE=GACGCCATGGATGGAAGAAA
PRIMER_LEFT_37=719,20
PRIMER_RIGHT_37=1345,20
PRIMER_LEFT_37_TM=55.835
PRIMER_RIGHT_37_TM=63.241
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=627
PRIMER_PAIR_38_PENALTY=7.501488
PRIMER_LEFT_38_PENALTY=4.165121
PRIMER_RIGHT_38_PENALTY=3.336368
PRIMER_LEFT_38_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_38_SEQUENCE=TGGGGAGAGAGAGAGAGGTAG
PRIMER_LEFT_38=719,20
PRIMER_RIGHT_38=1374,21
PRIMER_LEFT_38_TM=55.835
PRIMER_RIGHT_38_TM=57.664
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=57.143
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=656
PRIMER_PAIR_39_PENALTY=7.523654
PRIMER_LEFT_39_PENALTY=4.165121
PRIMER_RIGHT_39_PENALTY=3.358533
PRIMER_LEFT_39_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_39_SEQUENCE=TTACCAGATGAAGGATGGAGTTG
PRIMER_LEFT_39=719,20
PRIMER_RIGHT_39=1408,23
PRIMER_LEFT_39_TM=55.835
PRIMER_RIGHT_39_TM=60.359
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=43.478
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=690
PRIMER_PAIR_40_PENALTY=7.524484
PRIMER_LEFT_40_PENALTY=4.165121
PRIMER_RIGHT_40_PENALTY=3.359364
PRIMER_LEFT_40_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_40_SEQUENCE=TTCTTGGGGAGAGAGAGAGAGG
PRIMER_LEFT_40=719,20
PRIMER_RIGHT_40=1378,22
PRIMER_LEFT_40_TM=55.835
PRIMER_RIGHT_40_TM=61.359
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=54.545
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=660
PRIMER_PAIR_41_PENALTY=7.525043
PRIMER_LEFT_41_PENALTY=4.165121
PRIMER_RIGHT_41_PENALTY=3.359923
PRIMER_LEFT_41_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_41_SEQUENCE=TTCTTGGGGAGAGAGAGAGA
PRIMER_LEFT_41=719,20
PRIMER_RIGHT_41=1378,20
PRIMER_LEFT_41_TM=55.835
PRIMER_RIGHT_41_TM=56.640
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.6000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=660
PRIMER_PAIR_42_PENALTY=7.651436
PRIMER_LEFT_42_PENALTY=4.165121
PRIMER_RIGHT_42_PENALTY=3.486315
PRIMER_LEFT_42_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_42_SEQUENCE=AGGACCCAACAACATAAAGG
PRIMER_LEFT_42=719,20
PRIMER_RIGHT_42=1576,20
PRIMER_LEFT_42_TM=55.835
PRIMER_RIGHT_42_TM=56.514
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.6000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=858
PRIMER_PAIR_43_PENALTY=7.655384
PRIMER_LEFT_43_PENALTY=4.165121
PRIMER_RIGHT_43_PENALTY=3.490264
PRIMER_LEFT_43_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_43_SEQUENCE=GAGACGCCATGGATGGAAGA
PRIMER_LEFT_43=719,20
PRIMER_RIGHT_43=1347,20
PRIMER_LEFT_43_TM=55.835
PRIMER_RIGHT_43_TM=63.490
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.6000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=629
PRIMER_PAIR_44_PENALTY=7.959860
PRIMER_LEFT_44_PENALTY=4.165121
PRIMER_RIGHT_44_PENALTY=3.794740
PRIMER_LEFT_44_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_44_SEQUENCE=TCTTGGGGAGAGAGAGAGAG
PRIMER_LEFT_44=719,20
PRIMER_RIGHT_44=1377,20
PRIMER_LEFT_44_TM=55.835
PRIMER_RIGHT_44_TM=56.205
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=659
PRIMER_PAIR_45_PENALTY=7.991397
PRIMER_LEFT_45_PENALTY=4.165121
PRIMER_RIGHT_45_PENALTY=3.826277
PRIMER_LEFT_45_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_45_SEQUENCE=CAGATGAAGGATGGAGTTGGTG
PRIMER_LEFT_45=719,20
PRIMER_RIGHT_45=1404,22
PRIMER_LEFT_45_TM=55.835
PRIMER_RIGHT_45_TM=61.826
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=686
PRIMER_PAIR_46_PENALTY=8.097070
PRIMER_LEFT_46_PENALTY=4.165121
PRIMER_RIGHT_46_PENALTY=3.931950
PRIMER_LEFT_46_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_46_SEQUENCE=GAAAGGACCCAACAACATAAAGG
PRIMER_LEFT_46=719,20
PRIMER_RIGHT_46=1579,23
PRIMER_LEFT_46_TM=55.835
PRIMER_RIGHT_46_TM=60.932
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=43.478
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=861
PRIMER_PAIR_47_PENALTY=8.161421
PRIMER_LEFT_47_PENALTY=4.165121
PRIMER_RIGHT_47_PENALTY=3.996300
PRIMER_LEFT_47_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_47_SEQUENCE=AGATGAAGGATGGAGTTGGT
PRIMER_LEFT_47=719,20
PRIMER_RIGHT_47=1403,20
PRIMER_LEFT_47_TM=55.835
PRIMER_RIGHT_47_TM=56.004
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=685
PRIMER_PAIR_48_PENALTY=8.161421
PRIMER_LEFT_48_PENALTY=4.165121
PRIMER_RIGHT_48_PENALTY=3.996300
PRIMER_LEFT_48_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_48_SEQUENCE=ACCAGATGAAGGATGGAGTT
PRIMER_LEFT_48=719,20
PRIMER_RIGHT_48=1406,20
PRIMER_LEFT_48_TM=55.835
PRIMER_RIGHT_48_TM=56.004
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=688
PRIMER_PAIR_49_PENALTY=8.172337
PRIMER_LEFT_49_PENALTY=4.165121
PRIMER_RIGHT_49_PENALTY=4.007216
PRIMER_LEFT_49_SEQUENCE=TCCAAAATATGAGTGAGCGT
PRIMER_RIGHT_49_SEQUENCE=ACGCCATGGATGGAAGAAACT
PRIMER_LEFT_49=719,20
PRIMER_RIGHT_49=1344,21
PRIMER_LEFT_49_TM=55.835
PRIMER_RIGHT_49_TM=63.007
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=626
=
SEQUENCE_ID=MAGIC.175_4_9582520_9583248_can-0_ws-0_32419_1258573_TRUE
SEQUENCE_TARGET=950,828
SEQUENCE_TEMPLATE=AGAGATATATGCTTGANTGACCAAAATGTAAANAAAAAAAGCNTANNNNNNNNNNNNNNNNNNNNTGATAAATTGATTAGTACTGATTTANTCCATNNANATCNAACTCATACNANNCATTACAATCTTCTTANNGAGNCACAATACANANATTATATATNAATCTNCTACGATTATTTATATTATCATCNNNNATTATATTGTCCTAGAGGGCATTATTTAAATATATNAGCCATTGCATANAGAAACCATNATNAATACTATATGNTANAAACACACTGATGAAAAGTGAATCAATTAAGTAAGTAGAATGTTGGAGTCGTAGGTGGCTCCGGCNNTCCAATCAGCGGGAATAGCGTTNGGNGATTGGATCCAANTGANTCCNGCNCTTCCGTAGACTAAGAACCTCAATGTGAGAGTGCCTCTAGGTGGNTTCTGCAAATCATGAACCGCTCCAAACACTCTTCTCATACGTCTCCACTCTTTGCAATCCTCCTAAAATCATACATATACATGCATACCATNTAATAACGTTTAACTTTGTTTCACAAAATCATCTAAGAGTAAACTATATATTTTGATCCTCAAAAACANATATCTAAGTTTCTNAACATCTTTGATTAAATTTACCNGCCAGACTTCAACGGCGAGNATGTCATTAACACCACCAACGTACAAGACAAGGATGGCAAGATAATGAGGATTNTAGCTTTTCTCATGGATCTTATANACNAGATTATACCCTGCGTATCGGCAAGGGATCCTTTGGTACTCAACGTTGACTACACCGAAGGAGTAGAGCTGATTTTCTGTGCCGGGTCGCGCCATACGTCCGTACGCCTTAGGGCTTAAGATGAAGTCCGTNCCATCTCCTTCTCCNGAGTCCGTAGCCACTACGTATACTCCTTCCTCACTGCAGTGTGGTGGNATCTTGCACCTCACCNTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTTTAGNNNNTANTGACATAGNATANACTATNATAATATTTGATGAAANATTNATTGAGATAAGTAGATAANNNTATANAANAACCTGGTAACAAGCACCACAGCCAGTTCCATTGTTCCATAGNCGCCNTGAAACACCACTCACTTCACCGTTATTGATATCTCTTCCAAATTCTCCATACCCACAATGTCCNCCTGCANCATCNAAAAATTAATTNANTATNNNNNNNNNNNCGACAAANNTTNTNTATATTCTCATAATNGAAAGTTNTNATTACTAATTACGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAAAGAATTAAGGTACACGANNTATATAGCAATAACANTTTTAATACGTACGAGGATTTGCTTTGCAATCGGGGCTGCCATAATAAGTAGCTCTAGAGTTAACAAAATCGTCGGATAGNCAAAGGAGAGGCAAAANGANAATGACTTGAACAAATAGAAGAAGCAAAACGTGAGANTGCTTCATATTGTTGTTATCTTATCTATAAATATTGTAAGANTATTAATNNNNNNNNNTCTNTTTCTTAACANANGATNGGAANTGANNNNNNNATNNNTTATCACAAAAGATNGTGAAGATAGANGAGAGNCTTAGTAGGGTANTTATAGNTGCAGCTAGAGTGCTTATTANAATTATNCAATTGATNGAGCATCGTCACTNTTTTTNACTTTCCACTTGGCAAAAAGCTTAAAGCTNNCNNNNNNNNNNNNNNTAGNTNAACTTGGTATTAAATTAAATGTTTACATCATACATCTGCCAATATANNNTTAGGGTTGGCATAAACCCAGTAGATTCAAAAGTCGATTCTTANTATACGCANTTTATTNTTTGANNTNTNTAGTCTAGNTTAGTTAAGGAGNNTNNNNGNNNNNNAATTANCANTATANNAGTATTTTANNAGATTAAGTANGTGAACANTGTAAAGCNCAGANNATTGAGGTAACATAGTTCCAATAGATGCATAATCTCCATTTCCNTATAACACATACCGAAATTCAAACTAGTTTNAAGTGAAAAGATAAAAGATGNNNNNNNNNNNNNAAATCAATTCCTAATACTNTAGTTTCTTATAGGAGTATACNCTCTTTCTNTCTGNTATCAGCTCTNTATGTNGCATTCAATTGTAAAACANGNTTTNGATGAATANAATTTTACATTCNTANAAANAAAGANTATANCGCTATNNCNTTATTTTTGGTTGATGNTATATCGCTACTAACCCTTTGTAGACATCCTGTATTGATTATATATTTTNAANCNANTNTAGATATTTGTATAAAATATCNAAAATATGTAATGCAATAGANAATGGTNGGGTNTATAGCTTTTGGGNCTATTNTTTATTTGAAAAAGGATAGGTTGTGCCTCCAAAACGAGAAGTTCATGACACCTAATAGTAANTAGATTGGTTTACGATAGTTGTTTGGTGAATTGGATGAATGAACTCATAGAATATGCCAAGCGTGCTATTTTTTGGAANCACTAAGNCTTTNTTGTTTTTCACTACTAGCATTTTAGTAATANTAAGATT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_467057_467063_wil-2_can-0_142882_180450_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=NNNNNGTATCCGCAGTTGAANGCACNTNTNCNTTCGGAGCTTNNNTAATAATAANCCANAGCNNTAATAATAATAAACCAAAAGTAGGTCTCACCTGTGTTAACATGTGGAGTGACAAACTGACTATTCAAACAGCTATAACTCTCATGCAGGCCCCAATCGTTCTGCATATTCAACCATAAACAATTTTCAAATTAATTAGTTATATTGTTACCAAAACATTTGGNAATGATAATTGAAGNAAAGTTCCAAGAAAATTAAAGAAATCAAATAAACCTGCGAATCATCNTCGTTAAAAGATTTGGACGGATNCTCGAACAAGAGACGTCTCCTTTTGCTTCTCTCACCGGACTCGATAAGTGCGTCTTCGAGGTAACCTGTGGAGACGTCGGAGTAATCAATCTCCGGTGATCCGAAAATATGTTCCGGCGATCTATTCATGAAACCACATGTATCTGCAAACATATTACACTTTAATAAGAAAGTTCTTGAATAACTTGTAAATAAATACATGCGCGTCGATTCATATATNACATATGCNTATATGTATCAATATTTTACGATTGAAAAAACACAAAATATGTTTGTGTCTATATATTAATGTATATATTACCAAGATTAAAATCGGGCTTGAAGAGTGATCCAAGGTTATGGATATCCCAAGTGGGGAGAGAGTAGAGAAGCTCACTCATCTCTTCTTCTTTCTTTCTTTTTCTTTCNTTTTTTTGTTGTGTTTTCTCTTCTGAATTATTTTCTGATTGTTTTCTATGATATTAACTTATATCACTGCCTATTTATGTTGGTGGATTGTGTGTCTAAAGTGAATGTAACTGAGAATAAACAATAAACTTGGAAGAAAAAAAAAGTTAAGTAAAAAGTAGTGGAGAGGTTTAGAAGATGAAGACGAATTTGTACTCAATACAGTCAACGGAAAAAGAAATAAAGCAAAAGAAGAAATGTTGGATGGATAGTGTTTTTCCTCCTTGAGAAATNTAAAGGTNCTAAANNNNNNTTTCTCTTTAACAAACACACATTTTATGAAAATTTGAANTTTTCGGATGGNTANACAACCCCGAGATTAATACACAATCATTAGGTTATATATTATANCCANGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATATTTAGATTAGTAATATGTTTGCCATATATCATATGGTCACAATTACATAATCTGCATACTATATTAAATNTATGTGACTCCGCTACTTGGATGGTTTCAATAGTAATTAACTACTAGAAAAGNNNNACACANANNNNNNNNNNNNNNNNNAAAAAAAAAAAAANAAGTATTCGGGTGATCTATGTTATCTAACTTCGTAAATTNAAAAAACAAATGTATCAATTTTGTTATTATTTCAACGTTAATATTAGAGATGTTCCTGTCTAACTCCGTACATGTGTTTGAATTTTGTTAGGGTTTGGTTAGATGGGNGACCTTAATGCTAAAGGTTATGGTATCACATCATGCTGTTATTTTTACTGTTCCGCTTTAAGCAAATTTAGTAAATAATCTTATAATTTGACTCACACAAGATTAAACTAGAATAAGAAAATATCACATGTGAATAGTAAATAATGTCNGCAATTTTTAAACGTAATTGAAATTTAAATATATNTAATGGTTGATTGCGTGATTAAATGTTATTAACAATATTGTGNAATCATTTNAAAAAAATATTAAGTAATCAATTTTTCGGAATNAATAATCATGTGTGTAATGAGCTATGTGCACATAATCTAAGATCATCTTCAAATTNTAGTTGTTACAAATGATTAAAAAAAGGATATAAAACACGTTNATTTCACTGTTCCTGAGTTATGAAGTGGATGTNGTGTTTGTTTTACTAACGTTAGCTCAGTGGTAAAATATTGCTGATANAANNTTNNCNNNNATAAGTTATAACTTTTACTGTTATATAACGTTTAATNTTTTACATTTTACTGATATCAACGTCATGAGTAAAAAG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.084159
PRIMER_LEFT_0_PENALTY=0.010545
PRIMER_RIGHT_0_PENALTY=0.073614
PRIMER_LEFT_0_SEQUENCE=TTTGCTTCTCTCACCGGACT
PRIMER_RIGHT_0_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_0=333,20
PRIMER_RIGHT_0=1256,20
PRIMER_LEFT_0_TM=59.989
PRIMER_RIGHT_0_TM=60.074
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=11.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=924
PRIMER_PAIR_1_PENALTY=0.268972
PRIMER_LEFT_1_PENALTY=0.195358
PRIMER_RIGHT_1_PENALTY=0.073614
PRIMER_LEFT_1_SEQUENCE=GGGCTTGAAGAGTGATCCAA
PRIMER_RIGHT_1_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_1=626,20
PRIMER_RIGHT_1=1256,20
PRIMER_LEFT_1_TM=60.195
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=631
PRIMER_PAIR_2_PENALTY=0.448704
PRIMER_LEFT_2_PENALTY=0.375090
PRIMER_RIGHT_2_PENALTY=0.073614
PRIMER_LEFT_2_SEQUENCE=TTTTGCTTCTCTCACCGGAC
PRIMER_RIGHT_2_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_2=332,20
PRIMER_RIGHT_2=1256,20
PRIMER_LEFT_2_TM=60.375
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=925
PRIMER_PAIR_3_PENALTY=0.492299
PRIMER_LEFT_3_PENALTY=0.418685
PRIMER_RIGHT_3_PENALTY=0.073614
PRIMER_LEFT_3_SEQUENCE=CGGACTCGATAAGTGCGTCT
PRIMER_RIGHT_3_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_3=347,20
PRIMER_RIGHT_3=1256,20
PRIMER_LEFT_3_TM=60.419
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=910
PRIMER_PAIR_4_PENALTY=0.508982
PRIMER_LEFT_4_PENALTY=0.435368
PRIMER_RIGHT_4_PENALTY=0.073614
PRIMER_LEFT_4_SEQUENCE=ATGTTCCGGCGATCTATTCA
PRIMER_RIGHT_4_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_4=420,20
PRIMER_RIGHT_4=1256,20
PRIMER_LEFT_4_TM=60.435
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=837
PRIMER_PAIR_5_PENALTY=0.761577
PRIMER_LEFT_5_PENALTY=0.687963
PRIMER_RIGHT_5_PENALTY=0.073614
PRIMER_LEFT_5_SEQUENCE=AAAATCGGGCTTGAAGAGTG
PRIMER_RIGHT_5_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_5=620,20
PRIMER_RIGHT_5=1256,20
PRIMER_LEFT_5_TM=59.312
PRIMER_RIGHT_5_TM=60.074
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=11.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=637
PRIMER_PAIR_6_PENALTY=0.816021
PRIMER_LEFT_6_PENALTY=0.010545
PRIMER_RIGHT_6_PENALTY=0.805475
PRIMER_LEFT_6_SEQUENCE=TTTGCTTCTCTCACCGGACT
PRIMER_RIGHT_6_SEQUENCE=AAACCATCCAAGTAGCGGAG
PRIMER_LEFT_6=333,20
PRIMER_RIGHT_6=1255,20
PRIMER_LEFT_6_TM=59.989
PRIMER_RIGHT_6_TM=59.195
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=9.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=923
PRIMER_PAIR_7_PENALTY=1.000833
PRIMER_LEFT_7_PENALTY=0.195358
PRIMER_RIGHT_7_PENALTY=0.805475
PRIMER_LEFT_7_SEQUENCE=GGGCTTGAAGAGTGATCCAA
PRIMER_RIGHT_7_SEQUENCE=AAACCATCCAAGTAGCGGAG
PRIMER_LEFT_7=626,20
PRIMER_RIGHT_7=1255,20
PRIMER_LEFT_7_TM=60.195
PRIMER_RIGHT_7_TM=59.195
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=630
PRIMER_PAIR_8_PENALTY=1.022164
PRIMER_LEFT_8_PENALTY=0.010545
PRIMER_RIGHT_8_PENALTY=1.011619
PRIMER_LEFT_8_SEQUENCE=TTTGCTTCTCTCACCGGACT
PRIMER_RIGHT_8_SEQUENCE=AAACCATCCAAGTAGCGGAGT
PRIMER_LEFT_8=333,20
PRIMER_RIGHT_8=1255,21
PRIMER_LEFT_8_TM=59.989
PRIMER_RIGHT_8_TM=60.012
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=923
PRIMER_PAIR_9_PENALTY=1.031250
PRIMER_LEFT_9_PENALTY=0.010545
PRIMER_RIGHT_9_PENALTY=1.020705
PRIMER_LEFT_9_SEQUENCE=TTTGCTTCTCTCACCGGACT
PRIMER_RIGHT_9_SEQUENCE=TGAAACCATCCAAGTAGCGG
PRIMER_LEFT_9=333,20
PRIMER_RIGHT_9=1257,20
PRIMER_LEFT_9_TM=59.989
PRIMER_RIGHT_9_TM=61.021
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=11.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=925
PRIMER_PAIR_10_PENALTY=1.180565
PRIMER_LEFT_10_PENALTY=0.375090
PRIMER_RIGHT_10_PENALTY=0.805475
PRIMER_LEFT_10_SEQUENCE=TTTTGCTTCTCTCACCGGAC
PRIMER_RIGHT_10_SEQUENCE=AAACCATCCAAGTAGCGGAG
PRIMER_LEFT_10=332,20
PRIMER_RIGHT_10=1255,20
PRIMER_LEFT_10_TM=60.375
PRIMER_RIGHT_10_TM=59.195
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=9.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=924
PRIMER_PAIR_11_PENALTY=1.194950
PRIMER_LEFT_11_PENALTY=1.121337
PRIMER_RIGHT_11_PENALTY=0.073614
PRIMER_LEFT_11_SEQUENCE=CTGTGGAGACGTCGGAGTAA
PRIMER_RIGHT_11_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_11=377,20
PRIMER_RIGHT_11=1256,20
PRIMER_LEFT_11_TM=58.879
PRIMER_RIGHT_11_TM=60.074
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=880
PRIMER_PAIR_12_PENALTY=1.206543
PRIMER_LEFT_12_PENALTY=0.010545
PRIMER_RIGHT_12_PENALTY=1.195998
PRIMER_LEFT_12_SEQUENCE=TTTGCTTCTCTCACCGGACT
PRIMER_RIGHT_12_SEQUENCE=CCATCCAAGTAGCGGAGTCA
PRIMER_LEFT_12=333,20
PRIMER_RIGHT_12=1252,20
PRIMER_LEFT_12_TM=59.989
PRIMER_RIGHT_12_TM=61.196
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=920
PRIMER_PAIR_13_PENALTY=1.206977
PRIMER_LEFT_13_PENALTY=0.195358
PRIMER_RIGHT_13_PENALTY=1.011619
PRIMER_LEFT_13_SEQUENCE=GGGCTTGAAGAGTGATCCAA
PRIMER_RIGHT_13_SEQUENCE=AAACCATCCAAGTAGCGGAGT
PRIMER_LEFT_13=626,20
PRIMER_RIGHT_13=1255,21
PRIMER_LEFT_13_TM=60.195
PRIMER_RIGHT_13_TM=60.012
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=630
PRIMER_PAIR_14_PENALTY=1.216063
PRIMER_LEFT_14_PENALTY=0.195358
PRIMER_RIGHT_14_PENALTY=1.020705
PRIMER_LEFT_14_SEQUENCE=GGGCTTGAAGAGTGATCCAA
PRIMER_RIGHT_14_SEQUENCE=TGAAACCATCCAAGTAGCGG
PRIMER_LEFT_14=626,20
PRIMER_RIGHT_14=1257,20
PRIMER_LEFT_14_TM=60.195
PRIMER_RIGHT_14_TM=61.021
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=11.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=632
PRIMER_PAIR_15_PENALTY=1.224161
PRIMER_LEFT_15_PENALTY=0.418685
PRIMER_RIGHT_15_PENALTY=0.805475
PRIMER_LEFT_15_SEQUENCE=CGGACTCGATAAGTGCGTCT
PRIMER_RIGHT_15_SEQUENCE=AAACCATCCAAGTAGCGGAG
PRIMER_LEFT_15=347,20
PRIMER_RIGHT_15=1255,20
PRIMER_LEFT_15_TM=60.419
PRIMER_RIGHT_15_TM=59.195
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=909
PRIMER_PAIR_16_PENALTY=1.240844
PRIMER_LEFT_16_PENALTY=0.435368
PRIMER_RIGHT_16_PENALTY=0.805475
PRIMER_LEFT_16_SEQUENCE=ATGTTCCGGCGATCTATTCA
PRIMER_RIGHT_16_SEQUENCE=AAACCATCCAAGTAGCGGAG
PRIMER_LEFT_16=420,20
PRIMER_RIGHT_16=1255,20
PRIMER_LEFT_16_TM=60.435
PRIMER_RIGHT_16_TM=59.195
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=9.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=836
PRIMER_PAIR_17_PENALTY=1.251912
PRIMER_LEFT_17_PENALTY=1.178298
PRIMER_RIGHT_17_PENALTY=0.073614
PRIMER_LEFT_17_SEQUENCE=TTCTCTCACCGGACTCGATAA
PRIMER_RIGHT_17_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_17=338,21
PRIMER_RIGHT_17=1256,20
PRIMER_LEFT_17_TM=59.822
PRIMER_RIGHT_17_TM=60.074
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.9000
PRIMER_RIGHT_17_END_STABILITY=11.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=919
PRIMER_PAIR_18_PENALTY=1.330835
PRIMER_LEFT_18_PENALTY=1.257221
PRIMER_RIGHT_18_PENALTY=0.073614
PRIMER_LEFT_18_SEQUENCE=CGTCTCCTTTTGCTTCTCTCA
PRIMER_RIGHT_18_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_18=325,21
PRIMER_RIGHT_18=1256,20
PRIMER_LEFT_18_TM=59.743
PRIMER_RIGHT_18_TM=60.074
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=11.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=932
PRIMER_PAIR_19_PENALTY=1.333940
PRIMER_LEFT_19_PENALTY=1.260326
PRIMER_RIGHT_19_PENALTY=0.073614
PRIMER_LEFT_19_SEQUENCE=CCCAAGTGGGGAGAGAGTAG
PRIMER_RIGHT_19_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_19=658,20
PRIMER_RIGHT_19=1256,20
PRIMER_LEFT_19_TM=58.740
PRIMER_RIGHT_19_TM=60.074
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.4000
PRIMER_RIGHT_19_END_STABILITY=11.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=599
PRIMER_PAIR_20_PENALTY=1.335228
PRIMER_LEFT_20_PENALTY=1.261614
PRIMER_RIGHT_20_PENALTY=0.073614
PRIMER_LEFT_20_SEQUENCE=TCTCACCGGACTCGATAAGTG
PRIMER_RIGHT_20_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_20=341,21
PRIMER_RIGHT_20=1256,20
PRIMER_LEFT_20_TM=60.262
PRIMER_RIGHT_20_TM=60.074
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=916
PRIMER_PAIR_21_PENALTY=1.338923
PRIMER_LEFT_21_PENALTY=0.010545
PRIMER_RIGHT_21_PENALTY=1.328377
PRIMER_LEFT_21_SEQUENCE=TTTGCTTCTCTCACCGGACT
PRIMER_RIGHT_21_SEQUENCE=AACCATCCAAGTAGCGGAGT
PRIMER_LEFT_21=333,20
PRIMER_RIGHT_21=1254,20
PRIMER_LEFT_21_TM=59.989
PRIMER_RIGHT_21_TM=58.672
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=922
PRIMER_PAIR_22_PENALTY=1.386709
PRIMER_LEFT_22_PENALTY=0.375090
PRIMER_RIGHT_22_PENALTY=1.011619
PRIMER_LEFT_22_SEQUENCE=TTTTGCTTCTCTCACCGGAC
PRIMER_RIGHT_22_SEQUENCE=AAACCATCCAAGTAGCGGAGT
PRIMER_LEFT_22=332,20
PRIMER_RIGHT_22=1255,21
PRIMER_LEFT_22_TM=60.375
PRIMER_RIGHT_22_TM=60.012
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=924
PRIMER_PAIR_23_PENALTY=1.391356
PRIMER_LEFT_23_PENALTY=0.195358
PRIMER_RIGHT_23_PENALTY=1.195998
PRIMER_LEFT_23_SEQUENCE=GGGCTTGAAGAGTGATCCAA
PRIMER_RIGHT_23_SEQUENCE=CCATCCAAGTAGCGGAGTCA
PRIMER_LEFT_23=626,20
PRIMER_RIGHT_23=1252,20
PRIMER_LEFT_23_TM=60.195
PRIMER_RIGHT_23_TM=61.196
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=627
PRIMER_PAIR_24_PENALTY=1.395795
PRIMER_LEFT_24_PENALTY=0.375090
PRIMER_RIGHT_24_PENALTY=1.020705
PRIMER_LEFT_24_SEQUENCE=TTTTGCTTCTCTCACCGGAC
PRIMER_RIGHT_24_SEQUENCE=TGAAACCATCCAAGTAGCGG
PRIMER_LEFT_24=332,20
PRIMER_RIGHT_24=1257,20
PRIMER_LEFT_24_TM=60.375
PRIMER_RIGHT_24_TM=61.021
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.6000
PRIMER_RIGHT_24_END_STABILITY=11.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=926
PRIMER_PAIR_25_PENALTY=1.430304
PRIMER_LEFT_25_PENALTY=0.418685
PRIMER_RIGHT_25_PENALTY=1.011619
PRIMER_LEFT_25_SEQUENCE=CGGACTCGATAAGTGCGTCT
PRIMER_RIGHT_25_SEQUENCE=AAACCATCCAAGTAGCGGAGT
PRIMER_LEFT_25=347,20
PRIMER_RIGHT_25=1255,21
PRIMER_LEFT_25_TM=60.419
PRIMER_RIGHT_25_TM=60.012
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=909
PRIMER_PAIR_26_PENALTY=1.439390
PRIMER_LEFT_26_PENALTY=0.418685
PRIMER_RIGHT_26_PENALTY=1.020705
PRIMER_LEFT_26_SEQUENCE=CGGACTCGATAAGTGCGTCT
PRIMER_RIGHT_26_SEQUENCE=TGAAACCATCCAAGTAGCGG
PRIMER_LEFT_26=347,20
PRIMER_RIGHT_26=1257,20
PRIMER_LEFT_26_TM=60.419
PRIMER_RIGHT_26_TM=61.021
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.1000
PRIMER_RIGHT_26_END_STABILITY=11.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=911
PRIMER_PAIR_27_PENALTY=1.446987
PRIMER_LEFT_27_PENALTY=0.435368
PRIMER_RIGHT_27_PENALTY=1.011619
PRIMER_LEFT_27_SEQUENCE=ATGTTCCGGCGATCTATTCA
PRIMER_RIGHT_27_SEQUENCE=AAACCATCCAAGTAGCGGAGT
PRIMER_LEFT_27=420,20
PRIMER_RIGHT_27=1255,21
PRIMER_LEFT_27_TM=60.435
PRIMER_RIGHT_27_TM=60.012
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=836
PRIMER_PAIR_28_PENALTY=1.456073
PRIMER_LEFT_28_PENALTY=0.435368
PRIMER_RIGHT_28_PENALTY=1.020705
PRIMER_LEFT_28_SEQUENCE=ATGTTCCGGCGATCTATTCA
PRIMER_RIGHT_28_SEQUENCE=TGAAACCATCCAAGTAGCGG
PRIMER_LEFT_28=420,20
PRIMER_RIGHT_28=1257,20
PRIMER_LEFT_28_TM=60.435
PRIMER_RIGHT_28_TM=61.021
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=11.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=838
PRIMER_PAIR_29_PENALTY=1.493439
PRIMER_LEFT_29_PENALTY=0.687963
PRIMER_RIGHT_29_PENALTY=0.805475
PRIMER_LEFT_29_SEQUENCE=AAAATCGGGCTTGAAGAGTG
PRIMER_RIGHT_29_SEQUENCE=AAACCATCCAAGTAGCGGAG
PRIMER_LEFT_29=620,20
PRIMER_RIGHT_29=1255,20
PRIMER_LEFT_29_TM=59.312
PRIMER_RIGHT_29_TM=59.195
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=9.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=636
PRIMER_PAIR_30_PENALTY=1.503290
PRIMER_LEFT_30_PENALTY=1.429676
PRIMER_RIGHT_30_PENALTY=0.073614
PRIMER_LEFT_30_SEQUENCE=TATGTTCCGGCGATCTATTCA
PRIMER_RIGHT_30_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_30=419,21
PRIMER_RIGHT_30=1256,20
PRIMER_LEFT_30_TM=60.430
PRIMER_RIGHT_30_TM=60.074
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=11.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=838
PRIMER_PAIR_31_PENALTY=1.523735
PRIMER_LEFT_31_PENALTY=0.195358
PRIMER_RIGHT_31_PENALTY=1.328377
PRIMER_LEFT_31_SEQUENCE=GGGCTTGAAGAGTGATCCAA
PRIMER_RIGHT_31_SEQUENCE=AACCATCCAAGTAGCGGAGT
PRIMER_LEFT_31=626,20
PRIMER_RIGHT_31=1254,20
PRIMER_LEFT_31_TM=60.195
PRIMER_RIGHT_31_TM=58.672
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=629
PRIMER_PAIR_32_PENALTY=1.571088
PRIMER_LEFT_32_PENALTY=0.375090
PRIMER_RIGHT_32_PENALTY=1.195998
PRIMER_LEFT_32_SEQUENCE=TTTTGCTTCTCTCACCGGAC
PRIMER_RIGHT_32_SEQUENCE=CCATCCAAGTAGCGGAGTCA
PRIMER_LEFT_32=332,20
PRIMER_RIGHT_32=1252,20
PRIMER_LEFT_32_TM=60.375
PRIMER_RIGHT_32_TM=61.196
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.6000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=921
PRIMER_PAIR_33_PENALTY=1.614683
PRIMER_LEFT_33_PENALTY=0.418685
PRIMER_RIGHT_33_PENALTY=1.195998
PRIMER_LEFT_33_SEQUENCE=CGGACTCGATAAGTGCGTCT
PRIMER_RIGHT_33_SEQUENCE=CCATCCAAGTAGCGGAGTCA
PRIMER_LEFT_33=347,20
PRIMER_RIGHT_33=1252,20
PRIMER_LEFT_33_TM=60.419
PRIMER_RIGHT_33_TM=61.196
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=906
PRIMER_PAIR_34_PENALTY=1.631366
PRIMER_LEFT_34_PENALTY=0.435368
PRIMER_RIGHT_34_PENALTY=1.195998
PRIMER_LEFT_34_SEQUENCE=ATGTTCCGGCGATCTATTCA
PRIMER_RIGHT_34_SEQUENCE=CCATCCAAGTAGCGGAGTCA
PRIMER_LEFT_34=420,20
PRIMER_RIGHT_34=1252,20
PRIMER_LEFT_34_TM=60.435
PRIMER_RIGHT_34_TM=61.196
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=833
PRIMER_PAIR_35_PENALTY=1.686683
PRIMER_LEFT_35_PENALTY=1.613069
PRIMER_RIGHT_35_PENALTY=0.073614
PRIMER_LEFT_35_SEQUENCE=TCTCTCACCGGACTCGATAA
PRIMER_RIGHT_35_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_35=339,20
PRIMER_RIGHT_35=1256,20
PRIMER_LEFT_35_TM=58.387
PRIMER_RIGHT_35_TM=60.074
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=5.9000
PRIMER_RIGHT_35_END_STABILITY=11.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=918
PRIMER_PAIR_36_PENALTY=1.695537
PRIMER_LEFT_36_PENALTY=0.010545
PRIMER_RIGHT_36_PENALTY=1.684992
PRIMER_LEFT_36_SEQUENCE=TTTGCTTCTCTCACCGGACT
PRIMER_RIGHT_36_SEQUENCE=ATCCAAGTAGCGGAGTCACA
PRIMER_LEFT_36=333,20
PRIMER_RIGHT_36=1250,20
PRIMER_LEFT_36_TM=59.989
PRIMER_RIGHT_36_TM=58.315
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=918
PRIMER_PAIR_37_PENALTY=1.698120
PRIMER_LEFT_37_PENALTY=1.624506
PRIMER_RIGHT_37_PENALTY=0.073614
PRIMER_LEFT_37_SEQUENCE=CCCAAGTGGGGAGAGAGTAGA
PRIMER_RIGHT_37_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_37=658,21
PRIMER_RIGHT_37=1256,20
PRIMER_LEFT_37_TM=60.625
PRIMER_RIGHT_37_TM=60.074
PRIMER_LEFT_37_GC_PERCENT=57.143
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=5.4000
PRIMER_RIGHT_37_END_STABILITY=11.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=599
PRIMER_PAIR_38_PENALTY=1.699582
PRIMER_LEFT_38_PENALTY=0.687963
PRIMER_RIGHT_38_PENALTY=1.011619
PRIMER_LEFT_38_SEQUENCE=AAAATCGGGCTTGAAGAGTG
PRIMER_RIGHT_38_SEQUENCE=AAACCATCCAAGTAGCGGAGT
PRIMER_LEFT_38=620,20
PRIMER_RIGHT_38=1255,21
PRIMER_LEFT_38_TM=59.312
PRIMER_RIGHT_38_TM=60.012
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=636
PRIMER_PAIR_39_PENALTY=1.703467
PRIMER_LEFT_39_PENALTY=0.375090
PRIMER_RIGHT_39_PENALTY=1.328377
PRIMER_LEFT_39_SEQUENCE=TTTTGCTTCTCTCACCGGAC
PRIMER_RIGHT_39_SEQUENCE=AACCATCCAAGTAGCGGAGT
PRIMER_LEFT_39=332,20
PRIMER_RIGHT_39=1254,20
PRIMER_LEFT_39_TM=60.375
PRIMER_RIGHT_39_TM=58.672
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=923
PRIMER_PAIR_40_PENALTY=1.708668
PRIMER_LEFT_40_PENALTY=0.687963
PRIMER_RIGHT_40_PENALTY=1.020705
PRIMER_LEFT_40_SEQUENCE=AAAATCGGGCTTGAAGAGTG
PRIMER_RIGHT_40_SEQUENCE=TGAAACCATCCAAGTAGCGG
PRIMER_LEFT_40=620,20
PRIMER_RIGHT_40=1257,20
PRIMER_LEFT_40_TM=59.312
PRIMER_RIGHT_40_TM=61.021
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=11.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=638
PRIMER_PAIR_41_PENALTY=1.715358
PRIMER_LEFT_41_PENALTY=1.641744
PRIMER_RIGHT_41_PENALTY=0.073614
PRIMER_LEFT_41_SEQUENCE=TGGAGACGTCGGAGTAATCAA
PRIMER_RIGHT_41_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_41=380,21
PRIMER_RIGHT_41=1256,20
PRIMER_LEFT_41_TM=60.642
PRIMER_RIGHT_41_TM=60.074
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=11.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=877
PRIMER_PAIR_42_PENALTY=1.726037
PRIMER_LEFT_42_PENALTY=1.652423
PRIMER_RIGHT_42_PENALTY=0.073614
PRIMER_LEFT_42_SEQUENCE=TAAAATCGGGCTTGAAGAGTG
PRIMER_RIGHT_42_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_42=619,21
PRIMER_RIGHT_42=1256,20
PRIMER_LEFT_42_TM=59.348
PRIMER_RIGHT_42_TM=60.074
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=11.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=638
PRIMER_PAIR_43_PENALTY=1.747063
PRIMER_LEFT_43_PENALTY=0.418685
PRIMER_RIGHT_43_PENALTY=1.328377
PRIMER_LEFT_43_SEQUENCE=CGGACTCGATAAGTGCGTCT
PRIMER_RIGHT_43_SEQUENCE=AACCATCCAAGTAGCGGAGT
PRIMER_LEFT_43=347,20
PRIMER_RIGHT_43=1254,20
PRIMER_LEFT_43_TM=60.419
PRIMER_RIGHT_43_TM=58.672
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=908
PRIMER_PAIR_44_PENALTY=1.759102
PRIMER_LEFT_44_PENALTY=1.685488
PRIMER_RIGHT_44_PENALTY=0.073614
PRIMER_LEFT_44_SEQUENCE=CTCACCGGACTCGATAAGTG
PRIMER_RIGHT_44_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_44=342,20
PRIMER_RIGHT_44=1256,20
PRIMER_LEFT_44_TM=58.315
PRIMER_RIGHT_44_TM=60.074
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=11.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=915
PRIMER_PAIR_45_PENALTY=1.763746
PRIMER_LEFT_45_PENALTY=0.435368
PRIMER_RIGHT_45_PENALTY=1.328377
PRIMER_LEFT_45_SEQUENCE=ATGTTCCGGCGATCTATTCA
PRIMER_RIGHT_45_SEQUENCE=AACCATCCAAGTAGCGGAGT
PRIMER_LEFT_45=420,20
PRIMER_RIGHT_45=1254,20
PRIMER_LEFT_45_TM=60.435
PRIMER_RIGHT_45_TM=58.672
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=835
PRIMER_PAIR_46_PENALTY=1.848299
PRIMER_LEFT_46_PENALTY=1.774685
PRIMER_RIGHT_46_PENALTY=0.073614
PRIMER_LEFT_46_SEQUENCE=CCAAGATTAAAATCGGGCTTG
PRIMER_RIGHT_46_SEQUENCE=GAAACCATCCAAGTAGCGGA
PRIMER_LEFT_46=612,21
PRIMER_RIGHT_46=1256,20
PRIMER_LEFT_46_TM=60.775
PRIMER_RIGHT_46_TM=60.074
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=11.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=645
PRIMER_PAIR_47_PENALTY=1.880350
PRIMER_LEFT_47_PENALTY=0.195358
PRIMER_RIGHT_47_PENALTY=1.684992
PRIMER_LEFT_47_SEQUENCE=GGGCTTGAAGAGTGATCCAA
PRIMER_RIGHT_47_SEQUENCE=ATCCAAGTAGCGGAGTCACA
PRIMER_LEFT_47=626,20
PRIMER_RIGHT_47=1250,20
PRIMER_LEFT_47_TM=60.195
PRIMER_RIGHT_47_TM=58.315
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=625
PRIMER_PAIR_48_PENALTY=1.883961
PRIMER_LEFT_48_PENALTY=0.687963
PRIMER_RIGHT_48_PENALTY=1.195998
PRIMER_LEFT_48_SEQUENCE=AAAATCGGGCTTGAAGAGTG
PRIMER_RIGHT_48_SEQUENCE=CCATCCAAGTAGCGGAGTCA
PRIMER_LEFT_48=620,20
PRIMER_RIGHT_48=1252,20
PRIMER_LEFT_48_TM=59.312
PRIMER_RIGHT_48_TM=61.196
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=633
PRIMER_PAIR_49_PENALTY=1.926812
PRIMER_LEFT_49_PENALTY=1.121337
PRIMER_RIGHT_49_PENALTY=0.805475
PRIMER_LEFT_49_SEQUENCE=CTGTGGAGACGTCGGAGTAA
PRIMER_RIGHT_49_SEQUENCE=AAACCATCCAAGTAGCGGAG
PRIMER_LEFT_49=377,20
PRIMER_RIGHT_49=1255,20
PRIMER_LEFT_49_TM=58.879
PRIMER_RIGHT_49_TM=59.195
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.7000
PRIMER_RIGHT_49_END_STABILITY=9.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=879
=
SEQUENCE_ID=MAGIC.105_3_992086_992266_tsu-0_sf-2_11981_204152_TRUE
SEQUENCE_TARGET=950,280
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGGCCCATATATGTTTNAAACCCAAATTTNNGGATCTTGATTAACTNTGTTACTTTTCAATCATAATTAATAGAATTGAGNTGNNNNNNNNNAAAAANGAATTNACCTGTANAANAAAGAATGAAAGAACCATAACCATCCNAACNAAAAAAAAAAGTCAACTATGNNATGATATCATATTTTGGGAAAAGAATTTACAGAAAANAANAAAANTANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGAAAATGAAAATATNAAGTCACGTTTTTCATTTATTTATTCTTACTCACTTGCAAAAGATAATGTTAAATTAGTAAAANATCTGGAAANTTTAATNNTTTTTTCAGCAATGAACAAAGCAGAATTAAATGGTGCTCCAGAATTTGTAAGATCGGGTGGTGCTATTTAAATCCAATATCAGAAACANGTCGAACGAGACCAATAGAGCTAGCTTCTGATACATTTCGACTTTGGCGTTTCTCGATCTGTTGTTTCTGCNCCCAAATANANANNAAAANAAATCGGATCGGGGATTTCCCCAAATCGTAAAATTAGGTTTTGTTCTTCATTTCTAGCCCTAGCTTAGTTTTNTCGTTATCTTATTCATTAAGGTTTGATTNTACCTTTGTTTCGCTNCCCTCCTCGAATTTTGCAATTNCNTTATTAAAGGATCCTAGTTTTTCTGGCTCTTGTACTACGTCAAGTTTCATGGATTCTCGAGTTTCGATTCTCTTCGTCTGTGCNATAGCCNTGTCATGCTTTACGTCTGGGTCTGCTTCATCACCAGTGGACTTTTCCGTTTGCAATTACGAATTCGAGCTGTTTCGATTCGATTTAGAGGCCAAATGTCCTCCTTCTCTGTATCCAACTCCTCCTATTGAGGTACGTTTCTCATNTTGTGAGNTCNANGGTTTGTTTGNAATAAGCNTNAGNTTATGTAACTTANTATCTATGCGGATTAGACTACTCTTCCAATGCAAATATTGAATTAGGAAACANTGANNNNNNNTGATAACTATTAAGCATTGAAGTTGAAGCTCAGATNCGTCCTCTTGTTCTTCAAGTTTGGATTATCATCTTACCTACTACTGTGACNTGAAACTCTGTTTAGGTTCATAGTTTAATGCTCTTGTAATGCAGATTGTGTAGTAGCTGCTCTGTCCGCAAATATGATATAGCAAATTTTGAATAGGTGTTCTTTGTTTCTTCTAAGNGGATCTGAAGCTTTATTCTTAACTAAAGCAGTGTNAANATTTNCACTATGCAGGTAGATGGAGACTCACTAGACAGGCTAATGGCTTCACAACATGGAAATGCTTATATGTCTGTACTCTTTTATGCTTCCTGGTGCCCATTCTCACGTNCTGTGCGCCCTAAGTTTGATATGTTGAGTTCCATGTTTCCTCAAATACANCACCTANCTGTGGAGCATTCTCAAGCACTGCCATCGTAAGAAGTCTACTTANTATTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_13128258_13128780_oy-0_ler-0_1667327_80857_TRUE
SEQUENCE_TARGET=950,622
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAANAACATA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_10481425_10481608_zu-0_wil-2_889_622314_TRUE
SEQUENCE_TARGET=950,283
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_4102511_4102519_wu-0_sf-2_7521_1175081_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=AANATGACNTGTNGGTCTAGATAGTTTATGNGGAAGTGGGCAACAACAANCAATCTCTTTAGTTCATTGNTNGCCCGACAAAATTACTNCTAGTNTANAGCACGTATGGGTCTCAACCGGATCCATATAANGTCTCACGGATTGNNNNNAATGTNTCATAGAAACATATTTACATATCTATGTCATTGAAGTGTGTTGACCCTGCTTCTTGACTTTGGGCACATATACGACACAATCTATNGCATANGCTNAAGTCAAAACAAGATATAAAGATTGCATCAAGTAATCATTAATTGTTATCTTCCCGTTTGNCAGTNTTCTTAATATCAAGAAAGATATTAGTGTTTACCCATATTTTCAATAATTCCGGTGAATTCCCACTATCTAAANTTTTTTTTTTGTCTTCTATATAACTGGTCAACTTGAACAGGCAAAAGGCAATANGAATTAATTCNNNNNNNNNNNNNNNNNNNNNNAACTGATACAAGTATTCTCATANNNNNNNCCAAGNNTATTCACGCTGGTTAAGCACTTAGTTAGGGTGATATAATATAATGGTGCANTATTGAACTTNAAAGGCACATTAATTAATGAAATTACGAATTGCATNTAAATAGTACAANATAGTTTGGAGGAATTGNGATCTTCACATGTTGTGATGCGTTGAAAATAAGTAATACAAAATGCTTTTGTCCACTTCACTAAAATTGTTACAAAGATTCGGTGTTGGATTTTCCAAATTTCTATAAAGCCTGTGTTACCGTTAGAGCAGTGAAGGTCGCTCTCTATGCATGTTGAAAACGAAAGCTACAATCAACATCTTACCCCTTCAATGGGAGGGAGAGCTAATCTGTCTATATTATTTGGGTTTTCTATAAACTTCTAATATTCCCATATATATGTTTGGAAAATGATAGCAGATTGGGACANNNNNTTTATTTATAAGTTTCCTTCAAATCTAATAANATTTATAAGTCAATAATAATATTCTTTTCAAGCANTTCTAAGNANNTTTTTNTTNTTTAATACATTTTTAGATTGCAGAACCGAAATTAGTACCGGAGTCTTTCTAAGANNAGAGAAAGTGCAGCTACGTACTTTCTTATCTGTGCATTTTGGTATAAGAAGATTGNTTATTTAATTTTATAATGAGACGAGGTAGTTTAATTAGGGGAATCATCAAACATAGGATTAAGCATCTAATAAAAGTGATGTTGCAGCCAATAAAAGAAGTCTACGTTAAGAGATCAACAGAGCCACGTGTTTTCTAATCATGTAGCAATTGAATTTAACATGGTCGAAATNATAAAGATATTGTCACATATCTAAGTAAACATACTGTACATGTGGTTCGTGGGGATTTTGGAAACTAAATATAATGCAACGATGGCTACAAGACAGGTCCGACAGAATCTTTATAGAAATATTATAAATTACTAGACTAAATGGGGTTGACCTTTCCCACTGAACTGGGAAATTCTTACCCTTTTTCGTTTTTGATATAATTTAAAAGCACTACAAAATCCAATCATGAAAACGATTAACAAAGTTAAAGTAGGAAAATCAGTAATCTTCTTTTGGGAAGCTAATCAAAATAGGGACGTAGTTAGGCTTGTTATTGCATGCAACTGTGTAACAATAGCGATCTTNTTACCCAAATGTGAGATTTCACTTCCGTCGTCTCTTTCACAAGTTTTTCTTCCAGACAAGACATCCCCTCTCCCATCTTCTTAAAAAATATTTTCCATTTTCTTTTACATATACTTTTTCATTTTAGNTATACCNCAAAACAAAGGTGATACTGTAAAATTTAGTTCAAAACATACAATTGCCTAGCTTTATNGATAACTTGGTAGTGTTTTATTACCTAGTATAAACTACTAAATTTTACAATTNATTNATTTTTAGTTCTTTTTTATCTTAAAATATAATAATAAATAAAAATCTTTGATTCATCATCAACGCATACATTAAAATAACACAAATTTGGTAATCCAAGAAAANAATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.343512
PRIMER_LEFT_0_PENALTY=0.232835
PRIMER_RIGHT_0_PENALTY=0.110677
PRIMER_LEFT_0_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_0_SEQUENCE=AGATGGGAGAGGGGATGTCT
PRIMER_LEFT_0=829,20
PRIMER_RIGHT_0=1727,20
PRIMER_LEFT_0_TM=59.767
PRIMER_RIGHT_0_TM=59.889
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.5000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=899
PRIMER_PAIR_1_PENALTY=0.436269
PRIMER_LEFT_1_PENALTY=0.163602
PRIMER_RIGHT_1_PENALTY=0.272667
PRIMER_LEFT_1_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_1_SEQUENCE=GTCGGACCTGTCTTGTAGCC
PRIMER_LEFT_1=647,20
PRIMER_RIGHT_1=1407,20
PRIMER_LEFT_1_TM=60.164
PRIMER_RIGHT_1_TM=59.727
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=761
PRIMER_PAIR_2_PENALTY=0.505502
PRIMER_LEFT_2_PENALTY=0.232835
PRIMER_RIGHT_2_PENALTY=0.272667
PRIMER_LEFT_2_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_2_SEQUENCE=GTCGGACCTGTCTTGTAGCC
PRIMER_LEFT_2=829,20
PRIMER_RIGHT_2=1407,20
PRIMER_LEFT_2_TM=59.767
PRIMER_RIGHT_2_TM=59.727
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=579
PRIMER_PAIR_3_PENALTY=0.510999
PRIMER_LEFT_3_PENALTY=0.232835
PRIMER_RIGHT_3_PENALTY=0.278164
PRIMER_LEFT_3_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_3_SEQUENCE=GATGGGAGAGGGGATGTCTT
PRIMER_LEFT_3=829,20
PRIMER_RIGHT_3=1726,20
PRIMER_LEFT_3_TM=59.767
PRIMER_RIGHT_3_TM=60.278
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=898
PRIMER_PAIR_4_PENALTY=0.510999
PRIMER_LEFT_4_PENALTY=0.232835
PRIMER_RIGHT_4_PENALTY=0.278164
PRIMER_LEFT_4_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_4_SEQUENCE=GAAGATGGGAGAGGGGATGT
PRIMER_LEFT_4=829,20
PRIMER_RIGHT_4=1729,20
PRIMER_LEFT_4_TM=59.767
PRIMER_RIGHT_4_TM=60.278
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=901
PRIMER_PAIR_5_PENALTY=0.614445
PRIMER_LEFT_5_PENALTY=0.163602
PRIMER_RIGHT_5_PENALTY=0.450843
PRIMER_LEFT_5_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_5_SEQUENCE=CCAGTTCAGTGGGAAAGGTC
PRIMER_LEFT_5=647,20
PRIMER_RIGHT_5=1472,20
PRIMER_LEFT_5_TM=60.164
PRIMER_RIGHT_5_TM=59.549
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=826
PRIMER_PAIR_6_PENALTY=0.645057
PRIMER_LEFT_6_PENALTY=0.232835
PRIMER_RIGHT_6_PENALTY=0.412222
PRIMER_LEFT_6_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_6_SEQUENCE=AGAAGATGGGAGAGGGGATG
PRIMER_LEFT_6=829,20
PRIMER_RIGHT_6=1730,20
PRIMER_LEFT_6_TM=59.767
PRIMER_RIGHT_6_TM=60.412
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=2.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=902
PRIMER_PAIR_7_PENALTY=0.683679
PRIMER_LEFT_7_PENALTY=0.232835
PRIMER_RIGHT_7_PENALTY=0.450843
PRIMER_LEFT_7_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_7_SEQUENCE=CCAGTTCAGTGGGAAAGGTC
PRIMER_LEFT_7=829,20
PRIMER_RIGHT_7=1472,20
PRIMER_LEFT_7_TM=59.767
PRIMER_RIGHT_7_TM=59.549
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.5000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=644
PRIMER_PAIR_8_PENALTY=0.727646
PRIMER_LEFT_8_PENALTY=0.232835
PRIMER_RIGHT_8_PENALTY=0.494811
PRIMER_LEFT_8_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_8_SEQUENCE=GGGGATGTCTTGTCTGGAAG
PRIMER_LEFT_8=829,20
PRIMER_RIGHT_8=1717,20
PRIMER_LEFT_8_TM=59.767
PRIMER_RIGHT_8_TM=59.505
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.5000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=889
PRIMER_PAIR_9_PENALTY=0.727646
PRIMER_LEFT_9_PENALTY=0.232835
PRIMER_RIGHT_9_PENALTY=0.494811
PRIMER_LEFT_9_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_9_SEQUENCE=AGGGGATGTCTTGTCTGGAA
PRIMER_LEFT_9=829,20
PRIMER_RIGHT_9=1718,20
PRIMER_LEFT_9_TM=59.767
PRIMER_RIGHT_9_TM=59.505
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.5000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=890
PRIMER_PAIR_10_PENALTY=0.729727
PRIMER_LEFT_10_PENALTY=0.619050
PRIMER_RIGHT_10_PENALTY=0.110677
PRIMER_LEFT_10_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_10_SEQUENCE=AGATGGGAGAGGGGATGTCT
PRIMER_LEFT_10=833,20
PRIMER_RIGHT_10=1727,20
PRIMER_LEFT_10_TM=59.381
PRIMER_RIGHT_10_TM=59.889
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=895
PRIMER_PAIR_11_PENALTY=0.818051
PRIMER_LEFT_11_PENALTY=0.232835
PRIMER_RIGHT_11_PENALTY=0.585216
PRIMER_LEFT_11_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_11_SEQUENCE=TTGTGAAAGAGACGACGGAA
PRIMER_LEFT_11=829,20
PRIMER_RIGHT_11=1691,20
PRIMER_LEFT_11_TM=59.767
PRIMER_RIGHT_11_TM=59.415
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.5000
PRIMER_RIGHT_11_END_STABILITY=10.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=863
PRIMER_PAIR_12_PENALTY=0.824382
PRIMER_LEFT_12_PENALTY=0.163602
PRIMER_RIGHT_12_PENALTY=0.660780
PRIMER_LEFT_12_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_12_SEQUENCE=AAAACACGTGGCTCTGTTGA
PRIMER_LEFT_12=647,20
PRIMER_RIGHT_12=1266,20
PRIMER_LEFT_12_TM=60.164
PRIMER_RIGHT_12_TM=59.339
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=620
PRIMER_PAIR_13_PENALTY=0.841794
PRIMER_LEFT_13_PENALTY=0.163602
PRIMER_RIGHT_13_PENALTY=0.678193
PRIMER_LEFT_13_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_13_SEQUENCE=TTGGCTGCAACATCACTTTT
PRIMER_LEFT_13=647,20
PRIMER_RIGHT_13=1223,20
PRIMER_LEFT_13_TM=60.164
PRIMER_RIGHT_13_TM=59.322
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=577
PRIMER_PAIR_14_PENALTY=0.891717
PRIMER_LEFT_14_PENALTY=0.619050
PRIMER_RIGHT_14_PENALTY=0.272667
PRIMER_LEFT_14_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_14_SEQUENCE=GTCGGACCTGTCTTGTAGCC
PRIMER_LEFT_14=833,20
PRIMER_RIGHT_14=1407,20
PRIMER_LEFT_14_TM=59.381
PRIMER_RIGHT_14_TM=59.727
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=60.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=8.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=575
PRIMER_PAIR_15_PENALTY=0.893616
PRIMER_LEFT_15_PENALTY=0.232835
PRIMER_RIGHT_15_PENALTY=0.660780
PRIMER_LEFT_15_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_15_SEQUENCE=AAAACACGTGGCTCTGTTGA
PRIMER_LEFT_15=829,20
PRIMER_RIGHT_15=1266,20
PRIMER_LEFT_15_TM=59.767
PRIMER_RIGHT_15_TM=59.339
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.5000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=438
PRIMER_PAIR_16_PENALTY=0.897214
PRIMER_LEFT_16_PENALTY=0.619050
PRIMER_RIGHT_16_PENALTY=0.278164
PRIMER_LEFT_16_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_16_SEQUENCE=GATGGGAGAGGGGATGTCTT
PRIMER_LEFT_16=833,20
PRIMER_RIGHT_16=1726,20
PRIMER_LEFT_16_TM=59.381
PRIMER_RIGHT_16_TM=60.278
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=894
PRIMER_PAIR_17_PENALTY=0.897214
PRIMER_LEFT_17_PENALTY=0.619050
PRIMER_RIGHT_17_PENALTY=0.278164
PRIMER_LEFT_17_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_17_SEQUENCE=GAAGATGGGAGAGGGGATGT
PRIMER_LEFT_17=833,20
PRIMER_RIGHT_17=1729,20
PRIMER_LEFT_17_TM=59.381
PRIMER_RIGHT_17_TM=60.278
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.3000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=897
PRIMER_PAIR_18_PENALTY=0.910770
PRIMER_LEFT_18_PENALTY=0.249989
PRIMER_RIGHT_18_PENALTY=0.660780
PRIMER_LEFT_18_SEQUENCE=TCCGGTGAATTCCCACTATC
PRIMER_RIGHT_18_SEQUENCE=AAAACACGTGGCTCTGTTGA
PRIMER_LEFT_18=365,20
PRIMER_RIGHT_18=1266,20
PRIMER_LEFT_18_TM=59.750
PRIMER_RIGHT_18_TM=59.339
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=5.6000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=902
PRIMER_PAIR_19_PENALTY=0.928182
PRIMER_LEFT_19_PENALTY=0.249989
PRIMER_RIGHT_19_PENALTY=0.678193
PRIMER_LEFT_19_SEQUENCE=TCCGGTGAATTCCCACTATC
PRIMER_RIGHT_19_SEQUENCE=TTGGCTGCAACATCACTTTT
PRIMER_LEFT_19=365,20
PRIMER_RIGHT_19=1223,20
PRIMER_LEFT_19_TM=59.750
PRIMER_RIGHT_19_TM=59.322
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=859
PRIMER_PAIR_20_PENALTY=1.031272
PRIMER_LEFT_20_PENALTY=0.619050
PRIMER_RIGHT_20_PENALTY=0.412222
PRIMER_LEFT_20_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_20_SEQUENCE=AGAAGATGGGAGAGGGGATG
PRIMER_LEFT_20=833,20
PRIMER_RIGHT_20=1730,20
PRIMER_LEFT_20_TM=59.381
PRIMER_RIGHT_20_TM=60.412
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=898
PRIMER_PAIR_21_PENALTY=1.069894
PRIMER_LEFT_21_PENALTY=0.619050
PRIMER_RIGHT_21_PENALTY=0.450843
PRIMER_LEFT_21_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_21_SEQUENCE=CCAGTTCAGTGGGAAAGGTC
PRIMER_LEFT_21=833,20
PRIMER_RIGHT_21=1472,20
PRIMER_LEFT_21_TM=59.381
PRIMER_RIGHT_21_TM=59.549
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=640
PRIMER_PAIR_22_PENALTY=1.113861
PRIMER_LEFT_22_PENALTY=0.619050
PRIMER_RIGHT_22_PENALTY=0.494811
PRIMER_LEFT_22_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_22_SEQUENCE=GGGGATGTCTTGTCTGGAAG
PRIMER_LEFT_22=833,20
PRIMER_RIGHT_22=1717,20
PRIMER_LEFT_22_TM=59.381
PRIMER_RIGHT_22_TM=59.505
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=885
PRIMER_PAIR_23_PENALTY=1.113861
PRIMER_LEFT_23_PENALTY=0.619050
PRIMER_RIGHT_23_PENALTY=0.494811
PRIMER_LEFT_23_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_23_SEQUENCE=AGGGGATGTCTTGTCTGGAA
PRIMER_LEFT_23=833,20
PRIMER_RIGHT_23=1718,20
PRIMER_LEFT_23_TM=59.381
PRIMER_RIGHT_23_TM=59.505
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=886
PRIMER_PAIR_24_PENALTY=1.131748
PRIMER_LEFT_24_PENALTY=0.232835
PRIMER_RIGHT_24_PENALTY=0.898913
PRIMER_LEFT_24_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_24_SEQUENCE=AGAGGGGATGTCTTGTCTGG
PRIMER_LEFT_24=829,20
PRIMER_RIGHT_24=1720,20
PRIMER_LEFT_24_TM=59.767
PRIMER_RIGHT_24_TM=59.101
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.5000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=892
PRIMER_PAIR_25_PENALTY=1.204266
PRIMER_LEFT_25_PENALTY=0.619050
PRIMER_RIGHT_25_PENALTY=0.585216
PRIMER_LEFT_25_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_25_SEQUENCE=TTGTGAAAGAGACGACGGAA
PRIMER_LEFT_25=833,20
PRIMER_RIGHT_25=1691,20
PRIMER_LEFT_25_TM=59.381
PRIMER_RIGHT_25_TM=59.415
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=10.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=859
PRIMER_PAIR_26_PENALTY=1.225827
PRIMER_LEFT_26_PENALTY=0.232835
PRIMER_RIGHT_26_PENALTY=0.992992
PRIMER_LEFT_26_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_26_SEQUENCE=TGAAAGAGACGACGGAAGTG
PRIMER_LEFT_26=829,20
PRIMER_RIGHT_26=1688,20
PRIMER_LEFT_26_TM=59.767
PRIMER_RIGHT_26_TM=59.007
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.5000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=860
PRIMER_PAIR_27_PENALTY=1.225827
PRIMER_LEFT_27_PENALTY=0.232835
PRIMER_RIGHT_27_PENALTY=0.992992
PRIMER_LEFT_27_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_27_SEQUENCE=TGTGAAAGAGACGACGGAAG
PRIMER_LEFT_27=829,20
PRIMER_RIGHT_27=1690,20
PRIMER_LEFT_27_TM=59.767
PRIMER_RIGHT_27_TM=59.007
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.5000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=862
PRIMER_PAIR_28_PENALTY=1.225827
PRIMER_LEFT_28_PENALTY=0.232835
PRIMER_RIGHT_28_PENALTY=0.992992
PRIMER_LEFT_28_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_28_SEQUENCE=CTTGTGAAAGAGACGACGGA
PRIMER_LEFT_28=829,20
PRIMER_RIGHT_28=1692,20
PRIMER_LEFT_28_TM=59.767
PRIMER_RIGHT_28_TM=59.007
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.5000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=864
PRIMER_PAIR_29_PENALTY=1.250681
PRIMER_LEFT_29_PENALTY=0.232835
PRIMER_RIGHT_29_PENALTY=1.017846
PRIMER_LEFT_29_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_29_SEQUENCE=TTAAGAAGATGGGAGAGGGGA
PRIMER_LEFT_29=829,20
PRIMER_RIGHT_29=1733,21
PRIMER_LEFT_29_TM=59.767
PRIMER_RIGHT_29_TM=60.018
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.5000
PRIMER_RIGHT_29_END_STABILITY=10.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=905
PRIMER_PAIR_30_PENALTY=1.279831
PRIMER_LEFT_30_PENALTY=0.619050
PRIMER_RIGHT_30_PENALTY=0.660780
PRIMER_LEFT_30_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_30_SEQUENCE=AAAACACGTGGCTCTGTTGA
PRIMER_LEFT_30=833,20
PRIMER_RIGHT_30=1266,20
PRIMER_LEFT_30_TM=59.381
PRIMER_RIGHT_30_TM=59.339
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=434
PRIMER_PAIR_31_PENALTY=1.333804
PRIMER_LEFT_31_PENALTY=0.232835
PRIMER_RIGHT_31_PENALTY=1.100969
PRIMER_LEFT_31_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_31_SEQUENCE=GTGAAAGAGACGACGGAAGTG
PRIMER_LEFT_31=829,20
PRIMER_RIGHT_31=1689,21
PRIMER_LEFT_31_TM=59.767
PRIMER_RIGHT_31_TM=59.899
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=861
PRIMER_PAIR_32_PENALTY=1.333804
PRIMER_LEFT_32_PENALTY=0.232835
PRIMER_RIGHT_32_PENALTY=1.100969
PRIMER_LEFT_32_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_32_SEQUENCE=TGTGAAAGAGACGACGGAAGT
PRIMER_LEFT_32=829,20
PRIMER_RIGHT_32=1690,21
PRIMER_LEFT_32_TM=59.767
PRIMER_RIGHT_32_TM=59.899
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.5000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=862
PRIMER_PAIR_33_PENALTY=1.333804
PRIMER_LEFT_33_PENALTY=0.232835
PRIMER_RIGHT_33_PENALTY=1.100969
PRIMER_LEFT_33_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_33_SEQUENCE=ACTTGTGAAAGAGACGACGGA
PRIMER_LEFT_33=829,20
PRIMER_RIGHT_33=1693,21
PRIMER_LEFT_33_TM=59.767
PRIMER_RIGHT_33_TM=59.899
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.5000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=865
PRIMER_PAIR_34_PENALTY=1.338920
PRIMER_LEFT_34_PENALTY=1.228243
PRIMER_RIGHT_34_PENALTY=0.110677
PRIMER_LEFT_34_SEQUENCE=TTAGAGCAGTGAAGGTCGCTC
PRIMER_RIGHT_34_SEQUENCE=AGATGGGAGAGGGGATGTCT
PRIMER_LEFT_34=763,21
PRIMER_RIGHT_34=1727,20
PRIMER_LEFT_34_TM=59.772
PRIMER_RIGHT_34_TM=59.889
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.9000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=965
PRIMER_PAIR_35_PENALTY=1.364790
PRIMER_LEFT_35_PENALTY=0.163602
PRIMER_RIGHT_35_PENALTY=1.201188
PRIMER_LEFT_35_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_35_SEQUENCE=CTTGTAGCCATCGTTGCATT
PRIMER_LEFT_35=647,20
PRIMER_RIGHT_35=1396,20
PRIMER_LEFT_35_TM=60.164
PRIMER_RIGHT_35_TM=58.799
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=8.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=750
PRIMER_PAIR_36_PENALTY=1.366271
PRIMER_LEFT_36_PENALTY=0.232835
PRIMER_RIGHT_36_PENALTY=1.133436
PRIMER_LEFT_36_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_36_SEQUENCE=CAGTTCAGTGGGAAAGGTCAA
PRIMER_LEFT_36=829,20
PRIMER_RIGHT_36=1471,21
PRIMER_LEFT_36_TM=59.767
PRIMER_RIGHT_36_TM=60.133
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.5000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=643
PRIMER_PAIR_37_PENALTY=1.428463
PRIMER_LEFT_37_PENALTY=1.317786
PRIMER_RIGHT_37_PENALTY=0.110677
PRIMER_LEFT_37_SEQUENCE=ATGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_37_SEQUENCE=AGATGGGAGAGGGGATGTCT
PRIMER_LEFT_37=832,21
PRIMER_RIGHT_37=1727,20
PRIMER_LEFT_37_TM=59.682
PRIMER_RIGHT_37_TM=59.889
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=896
PRIMER_PAIR_38_PENALTY=1.434023
PRIMER_LEFT_38_PENALTY=0.232835
PRIMER_RIGHT_38_PENALTY=1.201188
PRIMER_LEFT_38_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_38_SEQUENCE=CTTGTAGCCATCGTTGCATT
PRIMER_LEFT_38=829,20
PRIMER_RIGHT_38=1396,20
PRIMER_LEFT_38_TM=59.767
PRIMER_RIGHT_38_TM=58.799
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.5000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=568
PRIMER_PAIR_39_PENALTY=1.451357
PRIMER_LEFT_39_PENALTY=0.163602
PRIMER_RIGHT_39_PENALTY=1.287755
PRIMER_LEFT_39_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_39_SEQUENCE=GATTCTGTCGGACCTGTCTTG
PRIMER_LEFT_39=647,20
PRIMER_RIGHT_39=1413,21
PRIMER_LEFT_39_TM=60.164
PRIMER_RIGHT_39_TM=59.712
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=767
PRIMER_PAIR_40_PENALTY=1.467396
PRIMER_LEFT_40_PENALTY=0.806616
PRIMER_RIGHT_40_PENALTY=0.660780
PRIMER_LEFT_40_SEQUENCE=ACTTGAACAGGCAAAAGGCA
PRIMER_RIGHT_40_SEQUENCE=AAAACACGTGGCTCTGTTGA
PRIMER_LEFT_40=420,20
PRIMER_RIGHT_40=1266,20
PRIMER_LEFT_40_TM=60.807
PRIMER_RIGHT_40_TM=59.339
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=847
PRIMER_PAIR_41_PENALTY=1.468781
PRIMER_LEFT_41_PENALTY=0.163602
PRIMER_RIGHT_41_PENALTY=1.305179
PRIMER_LEFT_41_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_41_SEQUENCE=CAGTTCAGTGGGAAAGGTCA
PRIMER_LEFT_41=647,20
PRIMER_RIGHT_41=1471,20
PRIMER_LEFT_41_TM=60.164
PRIMER_RIGHT_41_TM=58.695
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=825
PRIMER_PAIR_42_PENALTY=1.469221
PRIMER_LEFT_42_PENALTY=0.163602
PRIMER_RIGHT_42_PENALTY=1.305619
PRIMER_LEFT_42_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_42_SEQUENCE=CCCAGTTCAGTGGGAAAGGT
PRIMER_LEFT_42=647,20
PRIMER_RIGHT_42=1473,20
PRIMER_LEFT_42_TM=60.164
PRIMER_RIGHT_42_TM=61.306
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=827
PRIMER_PAIR_43_PENALTY=1.473265
PRIMER_LEFT_43_PENALTY=0.163602
PRIMER_RIGHT_43_PENALTY=1.309663
PRIMER_LEFT_43_SEQUENCE=CACATGTTGTGATGCGTTGA
PRIMER_RIGHT_43_SEQUENCE=GGAAAGGTCAACCCCATTTAG
PRIMER_LEFT_43=647,20
PRIMER_RIGHT_43=1461,21
PRIMER_LEFT_43_TM=60.164
PRIMER_RIGHT_43_TM=59.690
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=815
PRIMER_PAIR_44_PENALTY=1.484728
PRIMER_LEFT_44_PENALTY=0.232835
PRIMER_RIGHT_44_PENALTY=1.251892
PRIMER_LEFT_44_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_44_SEQUENCE=ATGGGAGAGGGGATGTCTTG
PRIMER_LEFT_44=829,20
PRIMER_RIGHT_44=1725,20
PRIMER_LEFT_44_TM=59.767
PRIMER_RIGHT_44_TM=61.252
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.5000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=897
PRIMER_PAIR_45_PENALTY=1.500910
PRIMER_LEFT_45_PENALTY=1.228243
PRIMER_RIGHT_45_PENALTY=0.272667
PRIMER_LEFT_45_SEQUENCE=TTAGAGCAGTGAAGGTCGCTC
PRIMER_RIGHT_45_SEQUENCE=GTCGGACCTGTCTTGTAGCC
PRIMER_LEFT_45=763,21
PRIMER_RIGHT_45=1407,20
PRIMER_LEFT_45_TM=59.772
PRIMER_RIGHT_45_TM=59.727
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=60.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.9000
PRIMER_RIGHT_45_END_STABILITY=8.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=645
PRIMER_PAIR_46_PENALTY=1.506407
PRIMER_LEFT_46_PENALTY=1.228243
PRIMER_RIGHT_46_PENALTY=0.278164
PRIMER_LEFT_46_SEQUENCE=TTAGAGCAGTGAAGGTCGCTC
PRIMER_RIGHT_46_SEQUENCE=GATGGGAGAGGGGATGTCTT
PRIMER_LEFT_46=763,21
PRIMER_RIGHT_46=1726,20
PRIMER_LEFT_46_TM=59.772
PRIMER_RIGHT_46_TM=60.278
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=964
PRIMER_PAIR_47_PENALTY=1.506407
PRIMER_LEFT_47_PENALTY=1.228243
PRIMER_RIGHT_47_PENALTY=0.278164
PRIMER_LEFT_47_SEQUENCE=TTAGAGCAGTGAAGGTCGCTC
PRIMER_RIGHT_47_SEQUENCE=GAAGATGGGAGAGGGGATGT
PRIMER_LEFT_47=763,21
PRIMER_RIGHT_47=1729,20
PRIMER_LEFT_47_TM=59.772
PRIMER_RIGHT_47_TM=60.278
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=967
PRIMER_PAIR_48_PENALTY=1.517964
PRIMER_LEFT_48_PENALTY=0.619050
PRIMER_RIGHT_48_PENALTY=0.898913
PRIMER_LEFT_48_SEQUENCE=TGGGAGGGAGAGCTAATCTG
PRIMER_RIGHT_48_SEQUENCE=AGAGGGGATGTCTTGTCTGG
PRIMER_LEFT_48=833,20
PRIMER_RIGHT_48=1720,20
PRIMER_LEFT_48_TM=59.381
PRIMER_RIGHT_48_TM=59.101
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=888
PRIMER_PAIR_49_PENALTY=1.520590
PRIMER_LEFT_49_PENALTY=0.232835
PRIMER_RIGHT_49_PENALTY=1.287755
PRIMER_LEFT_49_SEQUENCE=TCAATGGGAGGGAGAGCTAA
PRIMER_RIGHT_49_SEQUENCE=GATTCTGTCGGACCTGTCTTG
PRIMER_LEFT_49=829,20
PRIMER_RIGHT_49=1413,21
PRIMER_LEFT_49_TM=59.767
PRIMER_RIGHT_49_TM=59.712
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.5000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=585
=
SEQUENCE_ID=MAGIC.149_3_326618_327239_wil-2_no-0_326480_191764_TRUE
SEQUENCE_TARGET=950,721
SEQUENCE_TEMPLATE=AAAGAACATAACATTTTTCNATTCANTGNGTTCGGAAAAAAGTGAAANNGTTGAGATTAAGGAACCGTTATTTTATCCGTGTTTANTNNANTTNGTNTANCTAACTNTTTCNTANAACTTTTGAAAAGAATCAANAANATGTGGATAAGTTTNNATTTATGTAAGAATGTGANTNAATTNATTCGTGAGAGATTAATATAAAGAAATTCACAAGCGAATAACATTATCTTCGNGCGNNATTGTGGCGGCGGNAANTTAAAANGCAACAATTTGAGTTTGANNNNNNNTACGAAAGACTTAATTAGCTAAATCTGTNAAATAACANTATCTTCGTGCNGATGTCATCAAAACACTCTAGAATAGAAAAGGAAGAAAGCATATCTTTTGAGAAGATTCTGACTNTTATNTGANNTNTATATATTCTGTTGCATATTTGCTTTTGTGTTAAATGGTCCAATGGTATAATCCAATAATATCACCATATTCCGGATGACATGGTCCTTGGTTCTTTTTGTAAANATTTGGGCTTGTTGGGCTGACTTGACATTATGAAAAACACGAGAGCCCANGCTAATGGGCNTAACNANGNAANCGNCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGTTAGCTTTCNTACTCNTTCTCATCNTCATCTCCACAANATNCTTAGCTTGTCACTAGTTTTGGANTAATNGGTNGGNNNNNNNNNNNNNNNNNNNNNTTTGTAGATGANAGACTCATCATTGCTTCTTNNTGNTTNGNNNTTTGATGATGAATNTACTTTCGATTNCAGGGCAGCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATAAGGATTANNATTTTTGAGATATGTGANTTGCTGCTAGTTTAAAAGTAANGGTNTGATGGTGTGAGCCCAACNTTTTTNGGAAAGTAAATATTACAATCCANNNNNNNNNNNNANNCCTTCAAGNAAAAGGGAGGGAAGATGTGCATTTGTGTCAAGATGTGGTGGAAATTATCAGAGGGTTTATCAACGGCATGNGTATGAACACCTTGGTATGTCGTCACCACCACTCCTTCGTCTCCCCATTGCCTCTGCACTTGCTTCTTCACTCTGCATCCTTCTTCTGTNCACTTATAATAGCTCNNNNNNATATATATATTCATATTTTACATGTATGTATATGAGTCTCTTATTTGATCACCATANCAAGNANNNNNTATCATTCATTGATGATTANGTACGTACCTNGGGAATGGATTGTTCTTGACTGCTTTTTGGCCGTACTTCCTCCACCTNTATCCATCATCCAGTATATCAACCTGGCTTCTTGTCTGGAACGCGTACCTCGCTTCTCTCTCTTTCTTCTTCTTCTTACTCCTTGGCTTCTCTTCNGATGATGTTGTCGGCTGAGCAGANTTGTCGACCCCATGAAACTCTGTCANGCGAGGATCAACCGATGATGAACATGGAAACAACACNTCACNCCTCCTATCCTCNATTTCTTAACTCCCTCTGCGTTTATGGTGGGAACTTNAAAAAAGNCAAGGAGGNGAGGGAGAGAAAGAGAGACGTANACGGNAGCGAAGATGCCAGAGAAAGTNATATATATTGAAAATATGGCTACATTAGCGGATCCATCANANAAAAAGAGTTTTGTGGAAACAAAAAGGTGTGGCGGCTNNATCATTCTGATTTATATTATTATATATGCAACTCNTTCAAATTTTGGTTCAATGGATTCAANTTTCAAACATATTTCTTGGTCAAGAATCAANANGCATCTAGATCTCTGNGGTAATCATTCTTTTCTTATTTNNNTTACAATNNNNNNTAGCAAGGTTNNNAAGATAATATCCAATATCACGTGTATTTGAGGTTCACCTACCAAACTTGATTGAATTNAATAAACAATGAGCTCGANTGATGTNGAGATTGTTGACTATTTTGGCTCTTTGCTTTCTTCTATACTTGTCAAAAGTATNTAATCATTGATTCCATGGATTATTNATCCCTCCANAGTTATTCAAACTTGATTCATATTCACTTTTTACACACAACATAGAATTNNNATAATACTANGCNTTGATCACATGTAGGATTTGAGACGGGGTCGCGTAAGGGACTGCGCTCCATCCACCAAGACAGTGGTGCCTGTCATGAAGCGTGAGCCATCGCTGATCAAGTAGATAACAGTGGAGTAGAGGTCTGTNTCCGGGTTGAGCCACTGGCCGAGGGGTGCAGCGTCCTTGACCAGCTTCTGNGCTCGGTCTCTTCCCACAGAAGCTGTATACTCATCATCCAGATGCAGCCCTCTAGAGATCATGTTGACCCGTATCTTGTGCTTCCCGAGACTCATGGCTGATGCCTGAAACCCAATCATCAACAAACATATTCAAGTTACGAACGNGATGTGTCTAANTAGGAGCTAGGATGCATACCCGNACGAGCTGGTGAATAGCGGCAGAAGTTGAAGCGTAGGCATCAGCGCCAGGGTAAAGCGCCCTCTCTCCGCTGGCGATAGTGGCCATGAAGACAATGGAGCCTCCTGATCCATGGTCCTTCATCCTTGTGGCTACAG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_1612119_1612621_oy-0_po-0_262712_13675_TRUE
SEQUENCE_TARGET=950,602
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCCTAATANTNATGCTCNTAAGAGCACTACTATNGAAGNACGTCTCAATTNGTATTGGTTNGGNGTANTCCGGNGTGGCTNCANAGACGAATTGTAGAAGAANCTTNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTAATNNTTGGATTTTTGAACATTNCTCTNNTTTTNTTTTTAGTNAAACAACATTGCTCTNTTTCCGAAACAATCNGCTATGATATNGNCNTAATGTANTGTATGATCNTAGGCCCAATNCNTATTGGGCCAGTNAGCAATTGGCACAAATTGTATTCGTNGANTNTTTAGTGCGANGAGGACTANATAGTAAACTTGNGATNTCAACAATTTTNCGATTCCCACAGAAATGGCTTCNNNCTCNTCTTCGTCNCAGTACNATCCTCTGCCGTTGAAAGNCGGAGNGTNCAGAAACTGGGCNGANCTTCCGCCGGAACTAACATCTTCGATTCTTCTCCATCTCAGCACGATTGAGATACTNAGAAACGCTCAAATTGTATGCAGATCGTGGCNACGCGTCTGCAAAGACCCATCCATGTGGCGCAAAATCGACATGAGAAACAACGGAATCTTTGGTTACGACTACGANANNCCGTCACGCCGTTNATCTCAGCGAAGGTGGTTTNGTCGAAATCAACATGGTACACTTCGGCAACGATTCTCTCTACTCCTACATCGCCNAAAGGTTTNTNTCTATTCTTAGAATTTTNNNTTGGTTTCANNAANCTTTCTAGGGTTTAGATNNNATTATTAACAGGATTGGAGAAGNAATTGGGCGAATTAGNGCAANATTTAACAAAGCTTGTGNTTTTTNTTAACTGGATATNNNNNNNNNNNNNNNNNNNNNNGCCTTGCAAGTTAGAGGTCATGTTGTTGAATCTGTGGTTTTGATAAAGCAGTGTTCTCTNAGATTTAAGATNAATATGGTTATGTGGTATGTATATATGCTTCTGAGTTGNTAAGTATTGATANTTTATCNCAANTTTCTTTTTTTCAGATCAAGTAATCTGAGATGCCTTAGGCTTGCAATGTGCTATCCACTAANNGGCAATGGATTTGTCAGTGCAGTNATGAAGCTCTCATTCCTTGAGGAACTCGATATTTCACAGGGCTATACACAACTGGATTTGAAAGCTATAGGCCACNCTTGCCCACTACTGAAGNCATTCAAGCTAAACCGTCCTTCTTTCAGCCGGTTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_5423755_5424246_bur-0_po-0_13985_353617_TRUE
SEQUENCE_TARGET=950,591
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNCCAAATTCNNGAGANAATCGAGATCGAATTGTTTAAGAANCTTCTNCNNAAGTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGTATANATTNGCATANNAATTAGNNAGAGNTTATTCGNAGAAAGATGCATTCNAGTTTCTATTGNAGCAACTTCACANNGTTTATCTCTAGNCTTAAGCATCATGTAAGTCGTTTTGTATTTTTCAAAAGATTGATTTNTGTNNNTATATATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTCTAGTGTNGCAAGTNGGTGGAGAAAANATAANTATATGTNTNTATGTGTGTGTTNNGTTNTGTTGTTNTGTGAGATNAAAAGTTATTAACAATGTAGACAATTNNAAGANAAAATCGTTTNTTGTGTTTCTCTCTAANAAGAACTACAAGAGAGTGCAACAATAANAGAGAGACATAGTTAAAGAATGAGAGATCTAACCTCTAGGAGTGATAAAGAGGTTAGATGTGAGAATGTCAACACTGTNTTTNTGTTTACGTNNTCGATNTCTTGTAGTTTCTATTGTACAAGCANATCNCTTAAANTTGGTCATGTTATATCTTCACGCAAAGGATCTTCGTTCANGAGATTTTGNCTTNCCTCANTAATTNTTAATCATGGGTTTAATGGNTACTATAGTCTACCNTATTCTTTATTACCAAATAATANTGTTAGTTTGACTAAAANTTCAGATCAAAAGATTTTGTGGACATGNTTAATTTNGGTAAATATCTAGTAATAGTGNATATGGATTGNNACTTTGNAGTAGTTAATNAGGATANCAAAAATTACTANCNNNNNNNNAAAAANTTANNCATAATTTTNAGAAACTTTTTNTAATACATAGAAAGTAATCNNTTTTTTGCTGAACATACNTAGAAAANANTNNNNNNNNNNNNNNNNNNNNNNNNNNCTTNTATCNNNNNNNNNNNNNAANNNNNTAGTNNNNNNNNNNNATTATTGCAAAAAANAANNAAGCCTTTGNAATGTTGCNCACCGAAAACCCTACCTCTAGNCCCTATCTGATTCCCNAGAATTNNATCAAAGCAACTTGNATTCGATAATCTCCTCCTTGNTTACATTTCNGGATTCTCCATCTNTCTCCATAAGCTCNTCTTTNGATCGAGTACTTGATAATCTTCTCTCCTCTTGTGATGTCTCCGTTCAAGACNAACTCNTCNATCGTACTNTCGAGCGACTCTCNNTTCTTCTCCAATCCACCAAACGNTGCTCTNANAAACNAGCCACACTCCNCAATTCCATNTCNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4326013_4326165_mt-0_tsu-0_9664_48873_TRUE
SEQUENCE_TARGET=950,252
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_21716088_21716592_ct-1_ler-0_159740_118915_TRUE
SEQUENCE_TARGET=950,604
SEQUENCE_TEMPLATE=AATCACCTTCTTAATCACCACCCTCTNCGGCTCTCAACAGAACAACAANAAAAAAACAGCTTCCGTTGTCCTGTTCCGNCGAAATCGGACGGTCGAGATCAATCATGCATCGTAGAGCAGCAATTCAAGAATCGGATGACGAAGAAGATGAGACTTACAACGACGTCGTTCCTGAATCTCCTTCTTCTTGTGAAGACTCAAAGATCTCAAAACCAACTCCAAAGAAAAGGTTTGTNAACTTTTTTTNGGCTATTATTACGAATTGTTTTGGAAAACAAGATAAATGATCTGAAAATGTTAATAATTGTTGTGTAGTAGGAGGAACGTAGAGAAGAGAGTTGTCTCAGTTCCGATAGCTGACGTGGAAGGATCTAAGAGCAGAGGCGAAGTATATCCACCGTCCGATTCATGGGCCTGGAGAAAGTACGGACAAAAACCGATCAAAGGCTCGCCNTATCCCAGGTAAACAGACTCNTTTTTTAATTACCGTTGTGTCCTTTTTGTCGTTTTANTCAGCGGCGGCTTAGAAATGAGAAAGCGTAGANTAGCCTTGACGGTGGTTGGGCTAACGTACGCATCTCGTAGAGTTTTGTTGAATTACGGACGCGTTTGATATCGCGTGGGNACACGCGCCAGCTCCTTANTTCTTGGGGGGAGTTTTTGATGTTGTGAACTTGTAATTAAGGAACAATTAGAGATGNGTCATCACTCACTAATTGAATTTTTCTTTTTAACTAGTACTAGTATTATATTTTTGGATAACCTTGGGGGAAGTGTGAAATATTAAAGTAGCGGTAGATCTTGATTTCTAAGAAAAAGCCNCAAAAGGTAATGCGCACAAAAAGGAAAAGTATTTTANATATATATATATCTACGTGATTAACGTGCCAATATTATACCATTATCTAACATTTTATTCATTTGATTTCNTNGGGTTAATTTTAGTTTTTTCTGTATCAAAAACTAACANTTACATCTGTTAAAAAAGTTTGAATTATTTNATCATAAAATGTTATGTTCATAACTTTGNTTATATTTTCTAATGTATTTTTGCTAAAAGAGTTTAAAGTTAATCACTTNGAGATGAAAGTTAGTAACTTTAGTNGATCAATCTCTTTTTTCTTCTTTGCAGGGGATATTACAGATGTAGTAGCTCAAAAGGATGTCCGGCGAGGAAGCAGGTGGAGAGAAGCCGTGTGGACCCTTCTAAGCTTATGATTACTTACGCCTGCGACCACAATCACCCTTTCCCTTCCTCCTCCGCTAACACCAAATCCCACCACCGCTCCTCCGTCGTCCTCAAAACCGCAAAGAAAGAGGAAGAATACGAAGAGGAGGAAGAAGAACTAACCGTCACCGCCGCAGAGGAACCACCGGNGGGACTTGATCTAAGCCACGTAGACTCACCGTTGCTATTAGGCGGCTGCTACAGCGAANTCGGAGAGTTCGGGTGGTTCTACGACGCGTCGATCTCATCATCATCTGGTTCTTCGAATTTCCTCGANGTAACTCTAGAGAGAGGTTTTTCAGTAGGCCAAGAGGAAGATGAGTCTTTGTTCGGTGATCTCGGTGATTTACCTGATTGCGCCTCCGTGTTCCGCCGTGGGACTGTTGCGACGGAGGAGCAACATCGAAGATGTGATTTTGGCGCCATTCCTTTCTGTGATAGTTCTAGATGAGTTTGTGTGTGTAGCCAAAACCAAAAGAAAAAAACACAATTTTTTTATTTTCCACTGTAAAGGTGTATCAATGGTGGANTCATTTTTTNAAAAAAGGAAACCCNAATAATAAATTTAATATCATTTGAGAGAATTCAAGTACAATTAAAGATTCTAGCGGCGACGAGGATTACCGAAAAGGAAAGTTTCTTCCATTGCTCTTACCACAGAGAGTAGCTCTAATGTGAAGAGATCATTCGTTGTCATGTCTCCGGTGAGATTTGATCCATTAGCCGGAGGAGAAGAGTCTTGTTTTGTCGGCTTGAGTATGACGAATCTACAGACAGGACATTGTCCTTTNGTCTTTAACCATGGAACTATACATTCCTCGTGAAACATGTGTTTGCAAGGAGTGAGCATCACTGNTTCCTTGGGCTCGAAGTCTTCTAAACACACGGAGCATCTTTTGTCGTCTTCTTCACCTGNGAGATTTTGAGTGTTTCTTGAGCTTTCGCTTCCNGGGTTTTTGTTTCTGTAGTATAAGCTCAGGCTTCTGAGAAGGGTGCTTTTTGGAACTGGGTTATATGTTTCTTTTNTTAGATGTTTGAGGATCTCGTCTTCTTCATTGGTTTGTGATGATGATCTTGAGCTGCTCTCGGNTCTAGTTGATTGGTTGTGAACGGCTTGTCTTAGTGCCTCGAGGTGTTGTCTTCGCAGGTTGTTNCTGTTACGGATTGGAATCTGATGCCGAGTATGTGATGGTGGTACTAAATCTCTGCCAAGAACAATATATAATTTTTCAAGTTCTGAAAGAGGATGTAAAGACTATACTTTTGAAACAAAATC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.197145
PRIMER_LEFT_0_PENALTY=0.130883
PRIMER_RIGHT_0_PENALTY=0.066262
PRIMER_LEFT_0_SEQUENCE=AGGTAATGCGCACAAAAAGG
PRIMER_RIGHT_0_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_0=826,20
PRIMER_RIGHT_0=1574,20
PRIMER_LEFT_0_TM=60.131
PRIMER_RIGHT_0_TM=59.934
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=749
PRIMER_PAIR_1_PENALTY=0.309837
PRIMER_LEFT_1_PENALTY=0.243575
PRIMER_RIGHT_1_PENALTY=0.066262
PRIMER_LEFT_1_SEQUENCE=ATGCGCACAAAAAGGAAAAG
PRIMER_RIGHT_1_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_1=831,20
PRIMER_RIGHT_1=1574,20
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=744
PRIMER_PAIR_2_PENALTY=0.410811
PRIMER_LEFT_2_PENALTY=0.344549
PRIMER_RIGHT_2_PENALTY=0.066262
PRIMER_LEFT_2_SEQUENCE=TAACCTTGGGGGAAGTGTGA
PRIMER_RIGHT_2_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_2=759,20
PRIMER_RIGHT_2=1574,20
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=816
PRIMER_PAIR_3_PENALTY=0.457259
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.326376
PRIMER_LEFT_3_SEQUENCE=AGGTAATGCGCACAAAAAGG
PRIMER_RIGHT_3_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_3=826,20
PRIMER_RIGHT_3=1590,20
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.674
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=765
PRIMER_PAIR_4_PENALTY=0.569950
PRIMER_LEFT_4_PENALTY=0.243575
PRIMER_RIGHT_4_PENALTY=0.326376
PRIMER_LEFT_4_SEQUENCE=ATGCGCACAAAAAGGAAAAG
PRIMER_RIGHT_4_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_4=831,20
PRIMER_RIGHT_4=1590,20
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=59.674
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=760
PRIMER_PAIR_5_PENALTY=0.591749
PRIMER_LEFT_5_PENALTY=0.130883
PRIMER_RIGHT_5_PENALTY=0.460865
PRIMER_LEFT_5_SEQUENCE=AGGTAATGCGCACAAAAAGG
PRIMER_RIGHT_5_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_5=826,20
PRIMER_RIGHT_5=1575,20
PRIMER_LEFT_5_TM=60.131
PRIMER_RIGHT_5_TM=60.461
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=10.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=750
PRIMER_PAIR_6_PENALTY=0.615200
PRIMER_LEFT_6_PENALTY=0.548938
PRIMER_RIGHT_6_PENALTY=0.066262
PRIMER_LEFT_6_SEQUENCE=GGATAACCTTGGGGGAAGTG
PRIMER_RIGHT_6_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_6=756,20
PRIMER_RIGHT_6=1574,20
PRIMER_LEFT_6_TM=60.549
PRIMER_RIGHT_6_TM=59.934
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=819
PRIMER_PAIR_7_PENALTY=0.615200
PRIMER_LEFT_7_PENALTY=0.548938
PRIMER_RIGHT_7_PENALTY=0.066262
PRIMER_LEFT_7_SEQUENCE=TGGATAACCTTGGGGGAAGT
PRIMER_RIGHT_7_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_7=755,20
PRIMER_RIGHT_7=1574,20
PRIMER_LEFT_7_TM=60.549
PRIMER_RIGHT_7_TM=59.934
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=820
PRIMER_PAIR_8_PENALTY=0.670925
PRIMER_LEFT_8_PENALTY=0.344549
PRIMER_RIGHT_8_PENALTY=0.326376
PRIMER_LEFT_8_SEQUENCE=TAACCTTGGGGGAAGTGTGA
PRIMER_RIGHT_8_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_8=759,20
PRIMER_RIGHT_8=1590,20
PRIMER_LEFT_8_TM=60.345
PRIMER_RIGHT_8_TM=59.674
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=832
PRIMER_PAIR_9_PENALTY=0.704440
PRIMER_LEFT_9_PENALTY=0.243575
PRIMER_RIGHT_9_PENALTY=0.460865
PRIMER_LEFT_9_SEQUENCE=ATGCGCACAAAAAGGAAAAG
PRIMER_RIGHT_9_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_9=831,20
PRIMER_RIGHT_9=1575,20
PRIMER_LEFT_9_TM=60.244
PRIMER_RIGHT_9_TM=60.461
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=10.2000
PRIMER_PAIR_9_COMPL_ANY=2.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=745
PRIMER_PAIR_10_PENALTY=0.736721
PRIMER_LEFT_10_PENALTY=0.130883
PRIMER_RIGHT_10_PENALTY=0.605838
PRIMER_LEFT_10_SEQUENCE=AGGTAATGCGCACAAAAAGG
PRIMER_RIGHT_10_SEQUENCE=AGGCGCAATCAGGTAAATCA
PRIMER_LEFT_10=826,20
PRIMER_RIGHT_10=1589,20
PRIMER_LEFT_10_TM=60.131
PRIMER_RIGHT_10_TM=60.606
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=764
PRIMER_PAIR_11_PENALTY=0.805414
PRIMER_LEFT_11_PENALTY=0.344549
PRIMER_RIGHT_11_PENALTY=0.460865
PRIMER_LEFT_11_SEQUENCE=TAACCTTGGGGGAAGTGTGA
PRIMER_RIGHT_11_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_11=759,20
PRIMER_RIGHT_11=1575,20
PRIMER_LEFT_11_TM=60.345
PRIMER_RIGHT_11_TM=60.461
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=10.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=817
PRIMER_PAIR_12_PENALTY=0.848314
PRIMER_LEFT_12_PENALTY=0.782051
PRIMER_RIGHT_12_PENALTY=0.066262
PRIMER_LEFT_12_SEQUENCE=TGCGCACAAAAAGGAAAAGT
PRIMER_RIGHT_12_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_12=832,20
PRIMER_RIGHT_12=1574,20
PRIMER_LEFT_12_TM=60.782
PRIMER_RIGHT_12_TM=59.934
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=2.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=743
PRIMER_PAIR_13_PENALTY=0.849413
PRIMER_LEFT_13_PENALTY=0.243575
PRIMER_RIGHT_13_PENALTY=0.605838
PRIMER_LEFT_13_SEQUENCE=ATGCGCACAAAAAGGAAAAG
PRIMER_RIGHT_13_SEQUENCE=AGGCGCAATCAGGTAAATCA
PRIMER_LEFT_13=831,20
PRIMER_RIGHT_13=1589,20
PRIMER_LEFT_13_TM=60.244
PRIMER_RIGHT_13_TM=60.606
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=759
PRIMER_PAIR_14_PENALTY=0.875314
PRIMER_LEFT_14_PENALTY=0.548938
PRIMER_RIGHT_14_PENALTY=0.326376
PRIMER_LEFT_14_SEQUENCE=GGATAACCTTGGGGGAAGTG
PRIMER_RIGHT_14_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_14=756,20
PRIMER_RIGHT_14=1590,20
PRIMER_LEFT_14_TM=60.549
PRIMER_RIGHT_14_TM=59.674
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=835
PRIMER_PAIR_15_PENALTY=0.875314
PRIMER_LEFT_15_PENALTY=0.548938
PRIMER_RIGHT_15_PENALTY=0.326376
PRIMER_LEFT_15_SEQUENCE=TGGATAACCTTGGGGGAAGT
PRIMER_RIGHT_15_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_15=755,20
PRIMER_RIGHT_15=1590,20
PRIMER_LEFT_15_TM=60.549
PRIMER_RIGHT_15_TM=59.674
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=836
PRIMER_PAIR_16_PENALTY=0.888162
PRIMER_LEFT_16_PENALTY=0.130883
PRIMER_RIGHT_16_PENALTY=0.757279
PRIMER_LEFT_16_SEQUENCE=AGGTAATGCGCACAAAAAGG
PRIMER_RIGHT_16_SEQUENCE=ATCTTCGATGTTGCTCCTCC
PRIMER_LEFT_16=826,20
PRIMER_RIGHT_16=1637,20
PRIMER_LEFT_16_TM=60.131
PRIMER_RIGHT_16_TM=59.243
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=812
PRIMER_PAIR_17_PENALTY=0.939378
PRIMER_LEFT_17_PENALTY=0.873115
PRIMER_RIGHT_17_PENALTY=0.066262
PRIMER_LEFT_17_SEQUENCE=TTTTTGGATAACCTTGGGGG
PRIMER_RIGHT_17_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_17=751,20
PRIMER_RIGHT_17=1574,20
PRIMER_LEFT_17_TM=60.873
PRIMER_RIGHT_17_TM=59.934
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=12.4000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=824
PRIMER_PAIR_18_PENALTY=0.950387
PRIMER_LEFT_18_PENALTY=0.344549
PRIMER_RIGHT_18_PENALTY=0.605838
PRIMER_LEFT_18_SEQUENCE=TAACCTTGGGGGAAGTGTGA
PRIMER_RIGHT_18_SEQUENCE=AGGCGCAATCAGGTAAATCA
PRIMER_LEFT_18=759,20
PRIMER_RIGHT_18=1589,20
PRIMER_LEFT_18_TM=60.345
PRIMER_RIGHT_18_TM=60.606
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=831
PRIMER_PAIR_19_PENALTY=1.000854
PRIMER_LEFT_19_PENALTY=0.243575
PRIMER_RIGHT_19_PENALTY=0.757279
PRIMER_LEFT_19_SEQUENCE=ATGCGCACAAAAAGGAAAAG
PRIMER_RIGHT_19_SEQUENCE=ATCTTCGATGTTGCTCCTCC
PRIMER_LEFT_19=831,20
PRIMER_RIGHT_19=1637,20
PRIMER_LEFT_19_TM=60.244
PRIMER_RIGHT_19_TM=59.243
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.3000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=807
PRIMER_PAIR_20_PENALTY=1.009803
PRIMER_LEFT_20_PENALTY=0.548938
PRIMER_RIGHT_20_PENALTY=0.460865
PRIMER_LEFT_20_SEQUENCE=GGATAACCTTGGGGGAAGTG
PRIMER_RIGHT_20_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_20=756,20
PRIMER_RIGHT_20=1575,20
PRIMER_LEFT_20_TM=60.549
PRIMER_RIGHT_20_TM=60.461
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=10.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=820
PRIMER_PAIR_21_PENALTY=1.009803
PRIMER_LEFT_21_PENALTY=0.548938
PRIMER_RIGHT_21_PENALTY=0.460865
PRIMER_LEFT_21_SEQUENCE=TGGATAACCTTGGGGGAAGT
PRIMER_RIGHT_21_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_21=755,20
PRIMER_RIGHT_21=1575,20
PRIMER_LEFT_21_TM=60.549
PRIMER_RIGHT_21_TM=60.461
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=10.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=821
PRIMER_PAIR_22_PENALTY=1.065632
PRIMER_LEFT_22_PENALTY=0.999370
PRIMER_RIGHT_22_PENALTY=0.066262
PRIMER_LEFT_22_SEQUENCE=GGTAATGCGCACAAAAAGGA
PRIMER_RIGHT_22_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_22=827,20
PRIMER_RIGHT_22=1574,20
PRIMER_LEFT_22_TM=60.999
PRIMER_RIGHT_22_TM=59.934
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=748
PRIMER_PAIR_23_PENALTY=1.097592
PRIMER_LEFT_23_PENALTY=1.031330
PRIMER_RIGHT_23_PENALTY=0.066262
PRIMER_LEFT_23_SEQUENCE=TTGGATAACCTTGGGGGAAG
PRIMER_RIGHT_23_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_23=754,20
PRIMER_RIGHT_23=1574,20
PRIMER_LEFT_23_TM=61.031
PRIMER_RIGHT_23_TM=59.934
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=821
PRIMER_PAIR_24_PENALTY=1.101828
PRIMER_LEFT_24_PENALTY=0.344549
PRIMER_RIGHT_24_PENALTY=0.757279
PRIMER_LEFT_24_SEQUENCE=TAACCTTGGGGGAAGTGTGA
PRIMER_RIGHT_24_SEQUENCE=ATCTTCGATGTTGCTCCTCC
PRIMER_LEFT_24=759,20
PRIMER_RIGHT_24=1637,20
PRIMER_LEFT_24_TM=60.345
PRIMER_RIGHT_24_TM=59.243
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=879
PRIMER_PAIR_25_PENALTY=1.108427
PRIMER_LEFT_25_PENALTY=0.782051
PRIMER_RIGHT_25_PENALTY=0.326376
PRIMER_LEFT_25_SEQUENCE=TGCGCACAAAAAGGAAAAGT
PRIMER_RIGHT_25_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_25=832,20
PRIMER_RIGHT_25=1590,20
PRIMER_LEFT_25_TM=60.782
PRIMER_RIGHT_25_TM=59.674
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=759
PRIMER_PAIR_26_PENALTY=1.154776
PRIMER_LEFT_26_PENALTY=0.548938
PRIMER_RIGHT_26_PENALTY=0.605838
PRIMER_LEFT_26_SEQUENCE=GGATAACCTTGGGGGAAGTG
PRIMER_RIGHT_26_SEQUENCE=AGGCGCAATCAGGTAAATCA
PRIMER_LEFT_26=756,20
PRIMER_RIGHT_26=1589,20
PRIMER_LEFT_26_TM=60.549
PRIMER_RIGHT_26_TM=60.606
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=834
PRIMER_PAIR_27_PENALTY=1.154776
PRIMER_LEFT_27_PENALTY=0.548938
PRIMER_RIGHT_27_PENALTY=0.605838
PRIMER_LEFT_27_SEQUENCE=TGGATAACCTTGGGGGAAGT
PRIMER_RIGHT_27_SEQUENCE=AGGCGCAATCAGGTAAATCA
PRIMER_LEFT_27=755,20
PRIMER_RIGHT_27=1589,20
PRIMER_LEFT_27_TM=60.549
PRIMER_RIGHT_27_TM=60.606
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=835
PRIMER_PAIR_28_PENALTY=1.199491
PRIMER_LEFT_28_PENALTY=0.873115
PRIMER_RIGHT_28_PENALTY=0.326376
PRIMER_LEFT_28_SEQUENCE=TTTTTGGATAACCTTGGGGG
PRIMER_RIGHT_28_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_28=751,20
PRIMER_RIGHT_28=1590,20
PRIMER_LEFT_28_TM=60.873
PRIMER_RIGHT_28_TM=59.674
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=12.4000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=840
PRIMER_PAIR_29_PENALTY=1.242917
PRIMER_LEFT_29_PENALTY=0.782051
PRIMER_RIGHT_29_PENALTY=0.460865
PRIMER_LEFT_29_SEQUENCE=TGCGCACAAAAAGGAAAAGT
PRIMER_RIGHT_29_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_29=832,20
PRIMER_RIGHT_29=1575,20
PRIMER_LEFT_29_TM=60.782
PRIMER_RIGHT_29_TM=60.461
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=10.2000
PRIMER_PAIR_29_COMPL_ANY=2.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=744
PRIMER_PAIR_30_PENALTY=1.247460
PRIMER_LEFT_30_PENALTY=1.181197
PRIMER_RIGHT_30_PENALTY=0.066262
PRIMER_LEFT_30_SEQUENCE=GTAATGCGCACAAAAAGGAA
PRIMER_RIGHT_30_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_30=828,20
PRIMER_RIGHT_30=1574,20
PRIMER_LEFT_30_TM=58.819
PRIMER_RIGHT_30_TM=59.934
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=747
PRIMER_PAIR_31_PENALTY=1.302144
PRIMER_LEFT_31_PENALTY=1.235881
PRIMER_RIGHT_31_PENALTY=0.066262
PRIMER_LEFT_31_SEQUENCE=ATAACCTTGGGGGAAGTGTG
PRIMER_RIGHT_31_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_31=758,20
PRIMER_RIGHT_31=1574,20
PRIMER_LEFT_31_TM=58.764
PRIMER_RIGHT_31_TM=59.934
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=817
PRIMER_PAIR_32_PENALTY=1.306217
PRIMER_LEFT_32_PENALTY=0.548938
PRIMER_RIGHT_32_PENALTY=0.757279
PRIMER_LEFT_32_SEQUENCE=GGATAACCTTGGGGGAAGTG
PRIMER_RIGHT_32_SEQUENCE=ATCTTCGATGTTGCTCCTCC
PRIMER_LEFT_32=756,20
PRIMER_RIGHT_32=1637,20
PRIMER_LEFT_32_TM=60.549
PRIMER_RIGHT_32_TM=59.243
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=882
PRIMER_PAIR_33_PENALTY=1.306217
PRIMER_LEFT_33_PENALTY=0.548938
PRIMER_RIGHT_33_PENALTY=0.757279
PRIMER_LEFT_33_SEQUENCE=TGGATAACCTTGGGGGAAGT
PRIMER_RIGHT_33_SEQUENCE=ATCTTCGATGTTGCTCCTCC
PRIMER_LEFT_33=755,20
PRIMER_RIGHT_33=1637,20
PRIMER_LEFT_33_TM=60.549
PRIMER_RIGHT_33_TM=59.243
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=883
PRIMER_PAIR_34_PENALTY=1.325484
PRIMER_LEFT_34_PENALTY=1.259222
PRIMER_RIGHT_34_PENALTY=0.066262
PRIMER_LEFT_34_SEQUENCE=GGGGGAGTTTTTGATGTTGT
PRIMER_RIGHT_34_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_34=650,20
PRIMER_RIGHT_34=1574,20
PRIMER_LEFT_34_TM=58.741
PRIMER_RIGHT_34_TM=59.934
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=925
PRIMER_PAIR_35_PENALTY=1.325745
PRIMER_LEFT_35_PENALTY=0.999370
PRIMER_RIGHT_35_PENALTY=0.326376
PRIMER_LEFT_35_SEQUENCE=GGTAATGCGCACAAAAAGGA
PRIMER_RIGHT_35_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_35=827,20
PRIMER_RIGHT_35=1590,20
PRIMER_LEFT_35_TM=60.999
PRIMER_RIGHT_35_TM=59.674
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=764
PRIMER_PAIR_36_PENALTY=1.333981
PRIMER_LEFT_36_PENALTY=0.873115
PRIMER_RIGHT_36_PENALTY=0.460865
PRIMER_LEFT_36_SEQUENCE=TTTTTGGATAACCTTGGGGG
PRIMER_RIGHT_36_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_36=751,20
PRIMER_RIGHT_36=1575,20
PRIMER_LEFT_36_TM=60.873
PRIMER_RIGHT_36_TM=60.461
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=12.4000
PRIMER_RIGHT_36_END_STABILITY=10.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=825
PRIMER_PAIR_37_PENALTY=1.357706
PRIMER_LEFT_37_PENALTY=1.031330
PRIMER_RIGHT_37_PENALTY=0.326376
PRIMER_LEFT_37_SEQUENCE=TTGGATAACCTTGGGGGAAG
PRIMER_RIGHT_37_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_37=754,20
PRIMER_RIGHT_37=1590,20
PRIMER_LEFT_37_TM=61.031
PRIMER_RIGHT_37_TM=59.674
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=837
PRIMER_PAIR_38_PENALTY=1.387890
PRIMER_LEFT_38_PENALTY=0.782051
PRIMER_RIGHT_38_PENALTY=0.605838
PRIMER_LEFT_38_SEQUENCE=TGCGCACAAAAAGGAAAAGT
PRIMER_RIGHT_38_SEQUENCE=AGGCGCAATCAGGTAAATCA
PRIMER_LEFT_38=832,20
PRIMER_RIGHT_38=1589,20
PRIMER_LEFT_38_TM=60.782
PRIMER_RIGHT_38_TM=60.606
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=758
PRIMER_PAIR_39_PENALTY=1.450033
PRIMER_LEFT_39_PENALTY=1.383771
PRIMER_RIGHT_39_PENALTY=0.066262
PRIMER_LEFT_39_SEQUENCE=TTTGGATAACCTTGGGGGAA
PRIMER_RIGHT_39_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_39=753,20
PRIMER_RIGHT_39=1574,20
PRIMER_LEFT_39_TM=61.384
PRIMER_RIGHT_39_TM=59.934
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=8.4000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=822
PRIMER_PAIR_40_PENALTY=1.450033
PRIMER_LEFT_40_PENALTY=1.383771
PRIMER_RIGHT_40_PENALTY=0.066262
PRIMER_LEFT_40_SEQUENCE=TTTTGGATAACCTTGGGGGA
PRIMER_RIGHT_40_SEQUENCE=AATCACCGAGATCACCGAAC
PRIMER_LEFT_40=752,20
PRIMER_RIGHT_40=1574,20
PRIMER_LEFT_40_TM=61.384
PRIMER_RIGHT_40_TM=59.934
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=10.9000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=823
PRIMER_PAIR_41_PENALTY=1.460235
PRIMER_LEFT_41_PENALTY=0.999370
PRIMER_RIGHT_41_PENALTY=0.460865
PRIMER_LEFT_41_SEQUENCE=GGTAATGCGCACAAAAAGGA
PRIMER_RIGHT_41_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_41=827,20
PRIMER_RIGHT_41=1575,20
PRIMER_LEFT_41_TM=60.999
PRIMER_RIGHT_41_TM=60.461
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=10.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=749
PRIMER_PAIR_42_PENALTY=1.478954
PRIMER_LEFT_42_PENALTY=0.873115
PRIMER_RIGHT_42_PENALTY=0.605838
PRIMER_LEFT_42_SEQUENCE=TTTTTGGATAACCTTGGGGG
PRIMER_RIGHT_42_SEQUENCE=AGGCGCAATCAGGTAAATCA
PRIMER_LEFT_42=751,20
PRIMER_RIGHT_42=1589,20
PRIMER_LEFT_42_TM=60.873
PRIMER_RIGHT_42_TM=60.606
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=12.4000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=839
PRIMER_PAIR_43_PENALTY=1.492195
PRIMER_LEFT_43_PENALTY=1.031330
PRIMER_RIGHT_43_PENALTY=0.460865
PRIMER_LEFT_43_SEQUENCE=TTGGATAACCTTGGGGGAAG
PRIMER_RIGHT_43_SEQUENCE=AAATCACCGAGATCACCGAA
PRIMER_LEFT_43=754,20
PRIMER_RIGHT_43=1575,20
PRIMER_LEFT_43_TM=61.031
PRIMER_RIGHT_43_TM=60.461
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=10.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=822
PRIMER_PAIR_44_PENALTY=1.504674
PRIMER_LEFT_44_PENALTY=1.178298
PRIMER_RIGHT_44_PENALTY=0.326376
PRIMER_LEFT_44_SEQUENCE=GGGGAGTTTTTGATGTTGTGA
PRIMER_RIGHT_44_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_44=651,21
PRIMER_RIGHT_44=1590,20
PRIMER_LEFT_44_TM=59.822
PRIMER_RIGHT_44_TM=59.674
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=940
PRIMER_PAIR_45_PENALTY=1.507573
PRIMER_LEFT_45_PENALTY=1.181197
PRIMER_RIGHT_45_PENALTY=0.326376
PRIMER_LEFT_45_SEQUENCE=GTAATGCGCACAAAAAGGAA
PRIMER_RIGHT_45_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_45=828,20
PRIMER_RIGHT_45=1590,20
PRIMER_LEFT_45_TM=58.819
PRIMER_RIGHT_45_TM=59.674
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=763
PRIMER_PAIR_46_PENALTY=1.539330
PRIMER_LEFT_46_PENALTY=0.782051
PRIMER_RIGHT_46_PENALTY=0.757279
PRIMER_LEFT_46_SEQUENCE=TGCGCACAAAAAGGAAAAGT
PRIMER_RIGHT_46_SEQUENCE=ATCTTCGATGTTGCTCCTCC
PRIMER_LEFT_46=832,20
PRIMER_RIGHT_46=1637,20
PRIMER_LEFT_46_TM=60.782
PRIMER_RIGHT_46_TM=59.243
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=806
PRIMER_PAIR_47_PENALTY=1.562257
PRIMER_LEFT_47_PENALTY=1.235881
PRIMER_RIGHT_47_PENALTY=0.326376
PRIMER_LEFT_47_SEQUENCE=ATAACCTTGGGGGAAGTGTG
PRIMER_RIGHT_47_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_47=758,20
PRIMER_RIGHT_47=1590,20
PRIMER_LEFT_47_TM=58.764
PRIMER_RIGHT_47_TM=59.674
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=833
PRIMER_PAIR_48_PENALTY=1.584915
PRIMER_LEFT_48_PENALTY=0.130883
PRIMER_RIGHT_48_PENALTY=1.454032
PRIMER_LEFT_48_SEQUENCE=AGGTAATGCGCACAAAAAGG
PRIMER_RIGHT_48_SEQUENCE=TAAATCACCGAGATCACCGAA
PRIMER_LEFT_48=826,20
PRIMER_RIGHT_48=1576,21
PRIMER_LEFT_48_TM=60.131
PRIMER_RIGHT_48_TM=60.454
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=10.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=751
PRIMER_PAIR_49_PENALTY=1.585598
PRIMER_LEFT_49_PENALTY=1.259222
PRIMER_RIGHT_49_PENALTY=0.326376
PRIMER_LEFT_49_SEQUENCE=GGGGGAGTTTTTGATGTTGT
PRIMER_RIGHT_49_SEQUENCE=GAGGCGCAATCAGGTAAATC
PRIMER_LEFT_49=650,20
PRIMER_RIGHT_49=1590,20
PRIMER_LEFT_49_TM=58.741
PRIMER_RIGHT_49_TM=59.674
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=941
=
SEQUENCE_ID=MAGIC.433_3_12952863_12952899_wu-0_ws-0_7376_152336_TRUE
SEQUENCE_TARGET=950,136
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTCNNNGACTNGAGAATCNTCNCATGTGGTCGAGNNAACTTGACCNTGAGTAAGNNATNNTNGANCGTGCTCGAGAAGTTNTGCNCGANACCCNNAAAACTTTNNNAGCTANCGAATCNGTGAGTTGTNGTCGAATTGTGTGTTTGTTTGNATGTTTTCTAGAGCTAGTCTTTAGANTATATGCATGCTTACTCACTTAGACNTAACTAATCGGANTTNGGNTTAAGTTAGAACTTGAATCTTAAAGTGAATATGGGCATTTGNCGAACTTGATAATANTATCACTANNGAANGAACGGACTGGTGCATNACTAGGTNAAANGAATATGNNCTTAACTAAGTTTACTTGATGAATTGAGATATGATTCGAGGGCTTACTTACCNTGTTNGATNGTTATANTTATATGAGATATGCTANGAGGTTGTGANTAAATCCTTGNTATATTATGCATATATANTANGTGATTTGNTGCTTGATTGTCTATACTTGNTTTGGGNCATGGTAAACTACTTATATATAGTACTGCATGAGGTTATTGTGTCTAAGTGGTATTCAGGGTTTACCCNATACCATCATGCTTAGAGTAAGNNGCTGCCTNCCTTTTTGTGTTGTGATTNTGTNNGTAAGTCTTGATCCTGAGNGGTCACTTACATGATGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTNTGCANGTNCGTNTGTGTGGTAGCATACGTGTGGAGAACATGTATACACNTATGCNGGAGTTTGTGNTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8827992_8827998_po-0_sf-2_31494_3734_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=TGTACACCATATTCATAANGAACATATTCACNCATAAGNNCGTAACACACAAGATTACGATANATAGATTTACNTNTTGATNACGATNACGATCACAATGCAATGAACANATCNGACAAGCTTGTTTCTATTTTATAAAATANAAAAAAANNNNNNNNNNNNNNNNNNNNNAANAACCACGATATTAAAATACGGATAATGAAGATTGGATCTAAATTTATATTTTTGGCNAGAGAGTAGATTCGGACCCACTTTCTTACAAACAAAAAAACTTCGTCACCTTTACAATTTTCAATTATCCACCCAANAACAAATAATTAAAAATGCCTTAAAAATTCAAAAATAGTGGTTGTGATATTTTTCAAACATGTTCACACTTGCTAAAAGAACTAATAAAGNTTTTTTTTTTTTGAAGTGTCCATCAACTTTATATTATCCACGTANTTTAAACACATTAGATTNGGAATCAAAAACACCAAAATAANCATAGCCACTAGTTGTTTACCCACTGNACAAATCAAAANTTTCCAATATTTTTCTCTAGAAGGGAAATAAACGTTACCAGACAAAATCCAAAAAGCTCACTACCATCTACTATTGAGTTTTCTTGTCATCTTCTTTTTGGTCACGGTTTTAGTTTACTCTTGAGTCTCTTTCNTAGTTTCATAGCAAGAGCATCTTATNATTTCTTTCTNNNNNNNNNNNNNNNTTCACTGTAATAAANTTAGAAGGCTCACATTTTGCCAACTCCTCAACACTTTANTTTTCTCTTTTTNCTTCACGGATTTATAAANCAATAANTTTGCAGTAATCNTTTTNGTCGAATGATCTTTGAACATATTTCCTAAATACCCGGCAAGAAAAAATTCTGAATTTTTCTCATTAATTTCTCCAAANATACNAACTTTTCTTCTTCCTCTGTTTCTTCTTNCTNNTCTGTNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNAAGNTTTGTCTTTTANCNNTTNTAGNAGGTANTTGAANTGAATCTGNTNTTTTTTCTTTCTTCAGTGTTNTTTGTGACTGTGACATATAGTTCNTGTTTCTTTTTGGATGCTCNCTGTGTCAAACNAAAAAACAGAGCAAGTTGAGNTTTTTTGGAAATTTCCTACATCAACTTGCAAAGTCTGAATCTGTNTCTNTTGAATNNTTTTTTACAGGAAGAGTAGTAGTANCTTCTTNTTNACANTNTNATGGCTACAATAAACGATATTGGAGTAGCAGCAGCAATCAATATAGTCACAGCATTTGCNTTTCTCTTAGCTTTTGCTATATTCAGGATTCAACCAGTAAATGANAGAGTCTATTTCCCTAAATGGTATCTCAAGGGCTTAAGAAGTAGTTCTATACAAACTGGTGGCTTTGGTAGCAAATTCATCAATTTGGACTTCAGGTCCTATATTCGTTTCCTCAATTGGATGCCTGAAGCANTNAAAATGCCNGAACCNGANCTCGTCGATCACGCTGGACTNGATTCTGTTGTCTACTTGAGGATCTACTTACTCGGNNATATATATATATACTGATCTANNNNNNNNNNNNTCTTCNTTTTTTNTTCNGGTTTAAGAGATTNGAGTTCTTAATGGATAATTTGCAGGCTCAAGATCTTTTTCCCTATAGCTTGTGTTGCTTTTACNACAATGGTTCCTGTTAATTGGACAAACAAAGGATTGGATCGGTTAAGGCATTCCAATATAAGTTTCAGTGATATTGATAAACTCTCTTTATCAAATATACCAAATGGGTCACCTAGGTAAGAGANACTTCTCCGAGTTTGGTGGCTTTTTCTTTGTTTCANTATGGTTACTTANTCACTGGATCCTCCTCCTCATCTTCTGTAGATTTTGGGTGCATTTGTGTATGGCTTATGCGATTACCTTCTGGACNTGCTTTATCCTGAAAAGAGAGTACCAGAATATAGCTTTAATGAGACTACAGTTTCTTGCAAATGATCAAAGGAGACCTAACCAGTTCACT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.408159
PRIMER_LEFT_0_PENALTY=0.308722
PRIMER_RIGHT_0_PENALTY=0.099438
PRIMER_LEFT_0_SEQUENCE=CACATTTTGCCAACTCCTCA
PRIMER_RIGHT_0_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_0=734,20
PRIMER_RIGHT_0=1718,20
PRIMER_LEFT_0_TM=59.691
PRIMER_RIGHT_0_TM=59.901
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=985
PRIMER_PAIR_1_PENALTY=0.557437
PRIMER_LEFT_1_PENALTY=0.458000
PRIMER_RIGHT_1_PENALTY=0.099438
PRIMER_LEFT_1_SEQUENCE=TCCTAAATACCCGGCAAGAA
PRIMER_RIGHT_1_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_1=842,20
PRIMER_RIGHT_1=1718,20
PRIMER_LEFT_1_TM=59.542
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=877
PRIMER_PAIR_2_PENALTY=0.557437
PRIMER_LEFT_2_PENALTY=0.458000
PRIMER_RIGHT_2_PENALTY=0.099438
PRIMER_LEFT_2_SEQUENCE=TTCCTAAATACCCGGCAAGA
PRIMER_RIGHT_2_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_2=841,20
PRIMER_RIGHT_2=1718,20
PRIMER_LEFT_2_TM=59.542
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=878
PRIMER_PAIR_3_PENALTY=0.594961
PRIMER_LEFT_3_PENALTY=0.308722
PRIMER_RIGHT_3_PENALTY=0.286239
PRIMER_LEFT_3_SEQUENCE=CACATTTTGCCAACTCCTCA
PRIMER_RIGHT_3_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_3=734,20
PRIMER_RIGHT_3=1714,20
PRIMER_LEFT_3_TM=59.691
PRIMER_RIGHT_3_TM=60.286
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=981
PRIMER_PAIR_4_PENALTY=0.744239
PRIMER_LEFT_4_PENALTY=0.458000
PRIMER_RIGHT_4_PENALTY=0.286239
PRIMER_LEFT_4_SEQUENCE=TCCTAAATACCCGGCAAGAA
PRIMER_RIGHT_4_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_4=842,20
PRIMER_RIGHT_4=1714,20
PRIMER_LEFT_4_TM=59.542
PRIMER_RIGHT_4_TM=60.286
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=873
PRIMER_PAIR_5_PENALTY=0.744239
PRIMER_LEFT_5_PENALTY=0.458000
PRIMER_RIGHT_5_PENALTY=0.286239
PRIMER_LEFT_5_SEQUENCE=TTCCTAAATACCCGGCAAGA
PRIMER_RIGHT_5_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_5=841,20
PRIMER_RIGHT_5=1714,20
PRIMER_LEFT_5_TM=59.542
PRIMER_RIGHT_5_TM=60.286
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.8000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=874
PRIMER_PAIR_6_PENALTY=0.745164
PRIMER_LEFT_6_PENALTY=0.645726
PRIMER_RIGHT_6_PENALTY=0.099438
PRIMER_LEFT_6_SEQUENCE=GCTCACATTTTGCCAACTCC
PRIMER_RIGHT_6_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_6=731,20
PRIMER_RIGHT_6=1718,20
PRIMER_LEFT_6_TM=60.646
PRIMER_RIGHT_6_TM=59.901
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=988
PRIMER_PAIR_7_PENALTY=0.931965
PRIMER_LEFT_7_PENALTY=0.645726
PRIMER_RIGHT_7_PENALTY=0.286239
PRIMER_LEFT_7_SEQUENCE=GCTCACATTTTGCCAACTCC
PRIMER_RIGHT_7_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_7=731,20
PRIMER_RIGHT_7=1714,20
PRIMER_LEFT_7_TM=60.646
PRIMER_RIGHT_7_TM=60.286
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=7.8000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=984
PRIMER_PAIR_8_PENALTY=0.997827
PRIMER_LEFT_8_PENALTY=0.308722
PRIMER_RIGHT_8_PENALTY=0.689105
PRIMER_LEFT_8_SEQUENCE=CACATTTTGCCAACTCCTCA
PRIMER_RIGHT_8_SEQUENCE=TGCTACCAAAGCCACCAGTT
PRIMER_LEFT_8=734,20
PRIMER_RIGHT_8=1399,20
PRIMER_LEFT_8_TM=59.691
PRIMER_RIGHT_8_TM=60.689
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=666
PRIMER_PAIR_9_PENALTY=1.024745
PRIMER_LEFT_9_PENALTY=0.925307
PRIMER_RIGHT_9_PENALTY=0.099438
PRIMER_LEFT_9_SEQUENCE=CCTAAATACCCGGCAAGAAA
PRIMER_RIGHT_9_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_9=843,20
PRIMER_RIGHT_9=1718,20
PRIMER_LEFT_9_TM=59.075
PRIMER_RIGHT_9_TM=59.901
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=876
PRIMER_PAIR_10_PENALTY=1.024745
PRIMER_LEFT_10_PENALTY=0.925307
PRIMER_RIGHT_10_PENALTY=0.099438
PRIMER_LEFT_10_SEQUENCE=TTTCCTAAATACCCGGCAAG
PRIMER_RIGHT_10_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_10=840,20
PRIMER_RIGHT_10=1718,20
PRIMER_LEFT_10_TM=59.075
PRIMER_RIGHT_10_TM=59.901
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=879
PRIMER_PAIR_11_PENALTY=1.066382
PRIMER_LEFT_11_PENALTY=0.308722
PRIMER_RIGHT_11_PENALTY=0.757660
PRIMER_LEFT_11_SEQUENCE=CACATTTTGCCAACTCCTCA
PRIMER_RIGHT_11_SEQUENCE=GCTACCAAAGCCACCAGTTT
PRIMER_LEFT_11=734,20
PRIMER_RIGHT_11=1398,20
PRIMER_LEFT_11_TM=59.691
PRIMER_RIGHT_11_TM=59.242
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=665
PRIMER_PAIR_12_PENALTY=1.147105
PRIMER_LEFT_12_PENALTY=0.458000
PRIMER_RIGHT_12_PENALTY=0.689105
PRIMER_LEFT_12_SEQUENCE=TCCTAAATACCCGGCAAGAA
PRIMER_RIGHT_12_SEQUENCE=TGCTACCAAAGCCACCAGTT
PRIMER_LEFT_12=842,20
PRIMER_RIGHT_12=1399,20
PRIMER_LEFT_12_TM=59.542
PRIMER_RIGHT_12_TM=60.689
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=558
PRIMER_PAIR_13_PENALTY=1.147105
PRIMER_LEFT_13_PENALTY=0.458000
PRIMER_RIGHT_13_PENALTY=0.689105
PRIMER_LEFT_13_SEQUENCE=TTCCTAAATACCCGGCAAGA
PRIMER_RIGHT_13_SEQUENCE=TGCTACCAAAGCCACCAGTT
PRIMER_LEFT_13=841,20
PRIMER_RIGHT_13=1399,20
PRIMER_LEFT_13_TM=59.542
PRIMER_RIGHT_13_TM=60.689
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=559
PRIMER_PAIR_14_PENALTY=1.211546
PRIMER_LEFT_14_PENALTY=0.925307
PRIMER_RIGHT_14_PENALTY=0.286239
PRIMER_LEFT_14_SEQUENCE=CCTAAATACCCGGCAAGAAA
PRIMER_RIGHT_14_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_14=843,20
PRIMER_RIGHT_14=1714,20
PRIMER_LEFT_14_TM=59.075
PRIMER_RIGHT_14_TM=60.286
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.8000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=872
PRIMER_PAIR_15_PENALTY=1.211546
PRIMER_LEFT_15_PENALTY=0.925307
PRIMER_RIGHT_15_PENALTY=0.286239
PRIMER_LEFT_15_SEQUENCE=TTTCCTAAATACCCGGCAAG
PRIMER_RIGHT_15_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_15=840,20
PRIMER_RIGHT_15=1714,20
PRIMER_LEFT_15_TM=59.075
PRIMER_RIGHT_15_TM=60.286
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=7.8000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=875
PRIMER_PAIR_16_PENALTY=1.215660
PRIMER_LEFT_16_PENALTY=0.458000
PRIMER_RIGHT_16_PENALTY=0.757660
PRIMER_LEFT_16_SEQUENCE=TCCTAAATACCCGGCAAGAA
PRIMER_RIGHT_16_SEQUENCE=GCTACCAAAGCCACCAGTTT
PRIMER_LEFT_16=842,20
PRIMER_RIGHT_16=1398,20
PRIMER_LEFT_16_TM=59.542
PRIMER_RIGHT_16_TM=59.242
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=557
PRIMER_PAIR_17_PENALTY=1.215660
PRIMER_LEFT_17_PENALTY=0.458000
PRIMER_RIGHT_17_PENALTY=0.757660
PRIMER_LEFT_17_SEQUENCE=TTCCTAAATACCCGGCAAGA
PRIMER_RIGHT_17_SEQUENCE=GCTACCAAAGCCACCAGTTT
PRIMER_LEFT_17=841,20
PRIMER_RIGHT_17=1398,20
PRIMER_LEFT_17_TM=59.542
PRIMER_RIGHT_17_TM=59.242
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=558
PRIMER_PAIR_18_PENALTY=1.291325
PRIMER_LEFT_18_PENALTY=0.308722
PRIMER_RIGHT_18_PENALTY=0.982603
PRIMER_LEFT_18_SEQUENCE=CACATTTTGCCAACTCCTCA
PRIMER_RIGHT_18_SEQUENCE=GCATCCAATTGAGGAAACGA
PRIMER_LEFT_18=734,20
PRIMER_RIGHT_18=1450,20
PRIMER_LEFT_18_TM=59.691
PRIMER_RIGHT_18_TM=60.983
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=717
PRIMER_PAIR_19_PENALTY=1.324360
PRIMER_LEFT_19_PENALTY=0.308722
PRIMER_RIGHT_19_PENALTY=1.015638
PRIMER_LEFT_19_SEQUENCE=CACATTTTGCCAACTCCTCA
PRIMER_RIGHT_19_SEQUENCE=AGGCATCCAATTGAGGAAAC
PRIMER_LEFT_19=734,20
PRIMER_RIGHT_19=1452,20
PRIMER_LEFT_19_TM=59.691
PRIMER_RIGHT_19_TM=58.984
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=719
PRIMER_PAIR_20_PENALTY=1.333780
PRIMER_LEFT_20_PENALTY=1.234342
PRIMER_RIGHT_20_PENALTY=0.099438
PRIMER_LEFT_20_SEQUENCE=TTGCCAACTCCTCAACACTTT
PRIMER_RIGHT_20_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_20=740,21
PRIMER_RIGHT_20=1718,20
PRIMER_LEFT_20_TM=59.766
PRIMER_RIGHT_20_TM=59.901
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=979
PRIMER_PAIR_21_PENALTY=1.333780
PRIMER_LEFT_21_PENALTY=1.234342
PRIMER_RIGHT_21_PENALTY=0.099438
PRIMER_LEFT_21_SEQUENCE=TTTGCCAACTCCTCAACACTT
PRIMER_RIGHT_21_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_21=739,21
PRIMER_RIGHT_21=1718,20
PRIMER_LEFT_21_TM=59.766
PRIMER_RIGHT_21_TM=59.901
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=980
PRIMER_PAIR_22_PENALTY=1.333780
PRIMER_LEFT_22_PENALTY=1.234342
PRIMER_RIGHT_22_PENALTY=0.099438
PRIMER_LEFT_22_SEQUENCE=TTTTGCCAACTCCTCAACACT
PRIMER_RIGHT_22_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_22=738,21
PRIMER_RIGHT_22=1718,20
PRIMER_LEFT_22_TM=59.766
PRIMER_RIGHT_22_TM=59.901
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.1000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=981
PRIMER_PAIR_23_PENALTY=1.334831
PRIMER_LEFT_23_PENALTY=0.645726
PRIMER_RIGHT_23_PENALTY=0.689105
PRIMER_LEFT_23_SEQUENCE=GCTCACATTTTGCCAACTCC
PRIMER_RIGHT_23_SEQUENCE=TGCTACCAAAGCCACCAGTT
PRIMER_LEFT_23=731,20
PRIMER_RIGHT_23=1399,20
PRIMER_LEFT_23_TM=60.646
PRIMER_RIGHT_23_TM=60.689
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=669
PRIMER_PAIR_24_PENALTY=1.350908
PRIMER_LEFT_24_PENALTY=1.251471
PRIMER_RIGHT_24_PENALTY=0.099438
PRIMER_LEFT_24_SEQUENCE=TTTTGCCAACTCCTCAACAC
PRIMER_RIGHT_24_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_24=738,20
PRIMER_RIGHT_24=1718,20
PRIMER_LEFT_24_TM=58.749
PRIMER_RIGHT_24_TM=59.901
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=981
PRIMER_PAIR_25_PENALTY=1.373701
PRIMER_LEFT_25_PENALTY=1.274264
PRIMER_RIGHT_25_PENALTY=0.099438
PRIMER_LEFT_25_SEQUENCE=CTCACATTTTGCCAACTCCTC
PRIMER_RIGHT_25_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_25=732,21
PRIMER_RIGHT_25=1718,20
PRIMER_LEFT_25_TM=59.726
PRIMER_RIGHT_25_TM=59.901
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=987
PRIMER_PAIR_26_PENALTY=1.402388
PRIMER_LEFT_26_PENALTY=1.302950
PRIMER_RIGHT_26_PENALTY=0.099438
PRIMER_LEFT_26_SEQUENCE=TCACATTTTGCCAACTCCTC
PRIMER_RIGHT_26_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_26=733,20
PRIMER_RIGHT_26=1718,20
PRIMER_LEFT_26_TM=58.697
PRIMER_RIGHT_26_TM=59.901
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=986
PRIMER_PAIR_27_PENALTY=1.403324
PRIMER_LEFT_27_PENALTY=1.303887
PRIMER_RIGHT_27_PENALTY=0.099438
PRIMER_LEFT_27_SEQUENCE=CCTAAATACCCGGCAAGAAAA
PRIMER_RIGHT_27_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_27=843,21
PRIMER_RIGHT_27=1718,20
PRIMER_LEFT_27_TM=60.304
PRIMER_RIGHT_27_TM=59.901
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.3000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=876
PRIMER_PAIR_28_PENALTY=1.403386
PRIMER_LEFT_28_PENALTY=0.645726
PRIMER_RIGHT_28_PENALTY=0.757660
PRIMER_LEFT_28_SEQUENCE=GCTCACATTTTGCCAACTCC
PRIMER_RIGHT_28_SEQUENCE=GCTACCAAAGCCACCAGTTT
PRIMER_LEFT_28=731,20
PRIMER_RIGHT_28=1398,20
PRIMER_LEFT_28_TM=60.646
PRIMER_RIGHT_28_TM=59.242
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=668
PRIMER_PAIR_29_PENALTY=1.429603
PRIMER_LEFT_29_PENALTY=0.458000
PRIMER_RIGHT_29_PENALTY=0.971603
PRIMER_LEFT_29_SEQUENCE=TCCTAAATACCCGGCAAGAA
PRIMER_RIGHT_29_SEQUENCE=CAGGCATCCAATTGAGGAAA
PRIMER_LEFT_29=842,20
PRIMER_RIGHT_29=1453,20
PRIMER_LEFT_29_TM=59.542
PRIMER_RIGHT_29_TM=60.972
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=612
PRIMER_PAIR_30_PENALTY=1.429603
PRIMER_LEFT_30_PENALTY=0.458000
PRIMER_RIGHT_30_PENALTY=0.971603
PRIMER_LEFT_30_SEQUENCE=TTCCTAAATACCCGGCAAGA
PRIMER_RIGHT_30_SEQUENCE=CAGGCATCCAATTGAGGAAA
PRIMER_LEFT_30=841,20
PRIMER_RIGHT_30=1453,20
PRIMER_LEFT_30_TM=59.542
PRIMER_RIGHT_30_TM=60.972
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=7.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=613
PRIMER_PAIR_31_PENALTY=1.440603
PRIMER_LEFT_31_PENALTY=0.458000
PRIMER_RIGHT_31_PENALTY=0.982603
PRIMER_LEFT_31_SEQUENCE=TCCTAAATACCCGGCAAGAA
PRIMER_RIGHT_31_SEQUENCE=GCATCCAATTGAGGAAACGA
PRIMER_LEFT_31=842,20
PRIMER_RIGHT_31=1450,20
PRIMER_LEFT_31_TM=59.542
PRIMER_RIGHT_31_TM=60.983
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=609
PRIMER_PAIR_32_PENALTY=1.440603
PRIMER_LEFT_32_PENALTY=0.458000
PRIMER_RIGHT_32_PENALTY=0.982603
PRIMER_LEFT_32_SEQUENCE=TTCCTAAATACCCGGCAAGA
PRIMER_RIGHT_32_SEQUENCE=GCATCCAATTGAGGAAACGA
PRIMER_LEFT_32=841,20
PRIMER_RIGHT_32=1450,20
PRIMER_LEFT_32_TM=59.542
PRIMER_RIGHT_32_TM=60.983
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=610
PRIMER_PAIR_33_PENALTY=1.473638
PRIMER_LEFT_33_PENALTY=0.458000
PRIMER_RIGHT_33_PENALTY=1.015638
PRIMER_LEFT_33_SEQUENCE=TCCTAAATACCCGGCAAGAA
PRIMER_RIGHT_33_SEQUENCE=AGGCATCCAATTGAGGAAAC
PRIMER_LEFT_33=842,20
PRIMER_RIGHT_33=1452,20
PRIMER_LEFT_33_TM=59.542
PRIMER_RIGHT_33_TM=58.984
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=611
PRIMER_PAIR_34_PENALTY=1.473638
PRIMER_LEFT_34_PENALTY=0.458000
PRIMER_RIGHT_34_PENALTY=1.015638
PRIMER_LEFT_34_SEQUENCE=TTCCTAAATACCCGGCAAGA
PRIMER_RIGHT_34_SEQUENCE=AGGCATCCAATTGAGGAAAC
PRIMER_LEFT_34=841,20
PRIMER_RIGHT_34=1452,20
PRIMER_LEFT_34_TM=59.542
PRIMER_RIGHT_34_TM=58.984
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=7.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=612
PRIMER_PAIR_35_PENALTY=1.520581
PRIMER_LEFT_35_PENALTY=1.234342
PRIMER_RIGHT_35_PENALTY=0.286239
PRIMER_LEFT_35_SEQUENCE=TTGCCAACTCCTCAACACTTT
PRIMER_RIGHT_35_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_35=740,21
PRIMER_RIGHT_35=1714,20
PRIMER_LEFT_35_TM=59.766
PRIMER_RIGHT_35_TM=60.286
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.8000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=975
PRIMER_PAIR_36_PENALTY=1.520581
PRIMER_LEFT_36_PENALTY=1.234342
PRIMER_RIGHT_36_PENALTY=0.286239
PRIMER_LEFT_36_SEQUENCE=TTTGCCAACTCCTCAACACTT
PRIMER_RIGHT_36_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_36=739,21
PRIMER_RIGHT_36=1714,20
PRIMER_LEFT_36_TM=59.766
PRIMER_RIGHT_36_TM=60.286
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.8000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=976
PRIMER_PAIR_37_PENALTY=1.520581
PRIMER_LEFT_37_PENALTY=1.234342
PRIMER_RIGHT_37_PENALTY=0.286239
PRIMER_LEFT_37_SEQUENCE=TTTTGCCAACTCCTCAACACT
PRIMER_RIGHT_37_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_37=738,21
PRIMER_RIGHT_37=1714,20
PRIMER_LEFT_37_TM=59.766
PRIMER_RIGHT_37_TM=60.286
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.1000
PRIMER_RIGHT_37_END_STABILITY=7.8000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=977
PRIMER_PAIR_38_PENALTY=1.537710
PRIMER_LEFT_38_PENALTY=1.251471
PRIMER_RIGHT_38_PENALTY=0.286239
PRIMER_LEFT_38_SEQUENCE=TTTTGCCAACTCCTCAACAC
PRIMER_RIGHT_38_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_38=738,20
PRIMER_RIGHT_38=1714,20
PRIMER_LEFT_38_TM=58.749
PRIMER_RIGHT_38_TM=60.286
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=7.8000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=977
PRIMER_PAIR_39_PENALTY=1.560503
PRIMER_LEFT_39_PENALTY=1.274264
PRIMER_RIGHT_39_PENALTY=0.286239
PRIMER_LEFT_39_SEQUENCE=CTCACATTTTGCCAACTCCTC
PRIMER_RIGHT_39_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_39=732,21
PRIMER_RIGHT_39=1714,20
PRIMER_LEFT_39_TM=59.726
PRIMER_RIGHT_39_TM=60.286
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=7.8000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=983
PRIMER_PAIR_40_PENALTY=1.589189
PRIMER_LEFT_40_PENALTY=1.302950
PRIMER_RIGHT_40_PENALTY=0.286239
PRIMER_LEFT_40_SEQUENCE=TCACATTTTGCCAACTCCTC
PRIMER_RIGHT_40_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_40=733,20
PRIMER_RIGHT_40=1714,20
PRIMER_LEFT_40_TM=58.697
PRIMER_RIGHT_40_TM=60.286
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.8000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=982
PRIMER_PAIR_41_PENALTY=1.590126
PRIMER_LEFT_41_PENALTY=1.303887
PRIMER_RIGHT_41_PENALTY=0.286239
PRIMER_LEFT_41_SEQUENCE=CCTAAATACCCGGCAAGAAAA
PRIMER_RIGHT_41_SEQUENCE=GCCTTAACCGATCCAATCCT
PRIMER_LEFT_41=843,21
PRIMER_RIGHT_41=1714,20
PRIMER_LEFT_41_TM=60.304
PRIMER_RIGHT_41_TM=60.286
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=7.8000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=872
PRIMER_PAIR_42_PENALTY=1.614412
PRIMER_LEFT_42_PENALTY=0.925307
PRIMER_RIGHT_42_PENALTY=0.689105
PRIMER_LEFT_42_SEQUENCE=CCTAAATACCCGGCAAGAAA
PRIMER_RIGHT_42_SEQUENCE=TGCTACCAAAGCCACCAGTT
PRIMER_LEFT_42=843,20
PRIMER_RIGHT_42=1399,20
PRIMER_LEFT_42_TM=59.075
PRIMER_RIGHT_42_TM=60.689
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=557
PRIMER_PAIR_43_PENALTY=1.614412
PRIMER_LEFT_43_PENALTY=0.925307
PRIMER_RIGHT_43_PENALTY=0.689105
PRIMER_LEFT_43_SEQUENCE=TTTCCTAAATACCCGGCAAG
PRIMER_RIGHT_43_SEQUENCE=TGCTACCAAAGCCACCAGTT
PRIMER_LEFT_43=840,20
PRIMER_RIGHT_43=1399,20
PRIMER_LEFT_43_TM=59.075
PRIMER_RIGHT_43_TM=60.689
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=560
PRIMER_PAIR_44_PENALTY=1.617329
PRIMER_LEFT_44_PENALTY=0.645726
PRIMER_RIGHT_44_PENALTY=0.971603
PRIMER_LEFT_44_SEQUENCE=GCTCACATTTTGCCAACTCC
PRIMER_RIGHT_44_SEQUENCE=CAGGCATCCAATTGAGGAAA
PRIMER_LEFT_44=731,20
PRIMER_RIGHT_44=1453,20
PRIMER_LEFT_44_TM=60.646
PRIMER_RIGHT_44_TM=60.972
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=723
PRIMER_PAIR_45_PENALTY=1.628329
PRIMER_LEFT_45_PENALTY=0.645726
PRIMER_RIGHT_45_PENALTY=0.982603
PRIMER_LEFT_45_SEQUENCE=GCTCACATTTTGCCAACTCC
PRIMER_RIGHT_45_SEQUENCE=GCATCCAATTGAGGAAACGA
PRIMER_LEFT_45=731,20
PRIMER_RIGHT_45=1450,20
PRIMER_LEFT_45_TM=60.646
PRIMER_RIGHT_45_TM=60.983
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=720
PRIMER_PAIR_46_PENALTY=1.661364
PRIMER_LEFT_46_PENALTY=0.645726
PRIMER_RIGHT_46_PENALTY=1.015638
PRIMER_LEFT_46_SEQUENCE=GCTCACATTTTGCCAACTCC
PRIMER_RIGHT_46_SEQUENCE=AGGCATCCAATTGAGGAAAC
PRIMER_LEFT_46=731,20
PRIMER_RIGHT_46=1452,20
PRIMER_LEFT_46_TM=60.646
PRIMER_RIGHT_46_TM=58.984
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.6000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=722
PRIMER_PAIR_47_PENALTY=1.682967
PRIMER_LEFT_47_PENALTY=0.925307
PRIMER_RIGHT_47_PENALTY=0.757660
PRIMER_LEFT_47_SEQUENCE=CCTAAATACCCGGCAAGAAA
PRIMER_RIGHT_47_SEQUENCE=GCTACCAAAGCCACCAGTTT
PRIMER_LEFT_47=843,20
PRIMER_RIGHT_47=1398,20
PRIMER_LEFT_47_TM=59.075
PRIMER_RIGHT_47_TM=59.242
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=556
PRIMER_PAIR_48_PENALTY=1.682967
PRIMER_LEFT_48_PENALTY=0.925307
PRIMER_RIGHT_48_PENALTY=0.757660
PRIMER_LEFT_48_SEQUENCE=TTTCCTAAATACCCGGCAAG
PRIMER_RIGHT_48_SEQUENCE=GCTACCAAAGCCACCAGTTT
PRIMER_LEFT_48=840,20
PRIMER_RIGHT_48=1398,20
PRIMER_LEFT_48_TM=59.075
PRIMER_RIGHT_48_TM=59.242
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=559
PRIMER_PAIR_49_PENALTY=1.734382
PRIMER_LEFT_49_PENALTY=1.634945
PRIMER_RIGHT_49_PENALTY=0.099438
PRIMER_LEFT_49_SEQUENCE=ATTTCCTAAATACCCGGCAAG
PRIMER_RIGHT_49_SEQUENCE=GAATGCCTTAACCGATCCAA
PRIMER_LEFT_49=839,21
PRIMER_RIGHT_49=1718,20
PRIMER_LEFT_49_TM=59.365
PRIMER_RIGHT_49_TM=59.901
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=880
=
SEQUENCE_ID=MAGIC.446_2_4687205_4687247_po-0_bur-0_214479_113681_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_15988898_15989058_mt-0_bur-0_109072_17295_TRUE
SEQUENCE_TARGET=950,260
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGTGTGGTTNTAATGCTCATATGACTTGCTACNTTCAGAAGACCNAAAAATATANGNTTGTGTNATTCGAGNNNAACCATAACNATGATTTAGTGAGGACACCTATNAAGCNNNNNNNNNNNNNNNATCNNGAATTATCTATNTCTCNAAAACAACATGNAGATGATGCCGAGATGTNANGGANTNCAGCAAAAGCAACCGTTGAAATGATGAGNANAGAAGTTGGGNTCNGAGTNAATNNNNNNNTTNNNNNAAGAAAGATTATCAAAATTANATATACNNAAAGAGAATNGCAAAAATGGAAAAGNGAGATGNTGGAGCTGTTTTGGAATACTTTCAGAAAANNANGGTAATTCNTTTTTTTTATTCAATGCAANTTGATGANGATGATATGATCACTAATNTATTNTGGGNAGATNATCGGTCTATAAGTGATTACAATCNTTTTGGAGATGTCATTTGCTTTGACACAACTTATAAGACCAATGAATATGATAGANCCTTTGCTCCNTTTATCGGGGTCAATCATCACANGNAAACAGTTGTGTTNNGNGCTGCTCTCTTATATGATGAAACCAGTGAGTCATATAAGTGGNTTTTCGAGACTNTTCTTGGAGCAATTTCTNGAAANCAANCAAAAACAATTNTTACAGACCAATGTGCNNCAATGNCNAATACAATAGTAANTNNNNTNCCTGANACAAAANATAGATTATGTGTTNGACATATTTANCAAAATGATGCNAAGAAGTTGAGTCATGTANTTCNTGGAGCNNAACAATTTGCCATNGATTTTGGTAAGTGTGTATATNANCATGAGGAANAAGAAGACTNGTTATTGGCATGGAGTAATATGNTCCAGAAGCATAACTTGANANATGATAAATNNTTGNAGANTTTGTTTGANCTGAGAGAAAAATGTGCAATGGTATATGGACGTCATACTTTTACAGNAGATATNGTGAGCACACAACGTAGTAAAAGTATGAATAATATTTTGNAAAAAATACTTGAAGCATAGTTATAACTTNTTGTGCTTCTCTGAACACTNCGAGAGAGTGTTGGAAGATCNACGATATAAGGNATTAGTTGNTGATTTCAATATGATGCATACCTCGCCGGTATTATNTGCTACTNTAGAGATGTTGGAGATNTTGCAANATGCAGAGGAAGTGTATANACCCNAAATTTNTAGCTTGTTCNAGAAACAATAC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_12554315_12554688_wil-2_zu-0_23896_102353_TRUE
SEQUENCE_TARGET=950,473
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4720512_4720722_kn-0_rsch-4_20698_4398_TRUE
SEQUENCE_TARGET=950,310
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACAATCNTTGTNNAAGGTCCCGCCTGTGAGTNGGCCCTGGATGTTCCTGCCCNTTTNACGNTTTTNNTTTCTTGAGNNGCTTGCTTCAGTTGGAGACTNGTATGGNTGTTCTCTTNGGATCTCTNTGTTNCNANANNCATTTNCTCNNCTAGGGGTGATNCTTTTNCCNGANGNANTTCNCTTNNTTGGTGAGTTTTNNACTANCACCATNTTTCTTTTNCTTGATTNGGCCCCNNNNNNNNNNNNNNNNNNNNNNNNAATGGNCTTATTTAGAGGTGGNCGTCCTNTTTTNTCTTTTCCACCANNGNNNCANTAATGGGAGTTTCATCGTTGNGTANCATATNTGTTATTCCATTAATCTGACTTGAAANAGGCATTATAACAGATTGCTCTAGAGGTAAGGGATCTTNTTGNTTATTACAGGNAGNNNNCGNNATAATTTNNGCNGCANTNTTGNCATTAATCCTCTTGCTNNCCCTNNNNTNACNCTTTNTTTTNTNGCAGCACTCTCNNTNGNTCCGNACAGCTNGTNTACTGNACNGTTNCNTCCCTTAATTCTCCNATTACTTGTTCTGTGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNACTCCNTTTCCANAGGTGGTCTTNTTGTTCTAGANNTTTCANAGGAATCTTGNCTAAAATTGTCTCTTGNATNNNTCTTTTCACGCCATTGGAGNTNCTGGTTNTGGGANCTTCNTNCANAACTTGTTNAATTTTGAANCCTATCTNNGNGGTCCNTAGAACCANCNCAGAAGTTCGCTCTCTATTTNAGTAGCCTTCTNTGNNACTTGTTGTTCTNTACTCNCGTNATTTCCATTCANCNTGACTCCTTTCAACTTNCTCTCTTGNTGANTACGTTCTCTTTGNNGAANAAGATGAGTNNNNNGNANCATTAGGTCTTCCTGAGGGAGTATGATANGTTTTNGAATCTCNATNNCANTTNTTTNAGAAGANTNGTTAGTGTAGTTTGAATTTGNAGNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTNTACNGGTTNATAACNGATTTANAAANTAATNNNNNNNNNNNNNNNNNNNNNNNNTNTAANTNTTGAATTAAATNTATGTNATCAGNTTATNAATATANNTNGTTANTTNGCANNGNANAGATNAACTNAAATAAACCNA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17516934_17517121_rsch-4_can-0_2384_6719_TRUE
SEQUENCE_TARGET=950,287
SEQUENCE_TEMPLATE=TACCCNGTTTGGACCATCTTCTTTCACATCTTTTAAGTCAATCCGACCCATTGGNTTGTAGACACATTCAACATTTTGTGTCTTCAATTCGTTTTCAAGGTTTGAATTTTATAAGGAGCATTTGTGTTTGAGAAAGTCTTGAATATAAAAAAGAAGTTTCAATCGTGCACTGCTGACTTGTAAGATTNTTTTTNTTTTCTTGTAAATGTGTGNCTTGTAAGATATATAAATAAATTTNCATATTGGTTGATTTGTTGAATCAATCTTGAATATTGTAGACTTTTTAATTGATCATGACTAATAGTTGTTTTTTGAAAATTGTTTNTCATTTCATTAACACCAAACAATAAAGCAACACAAATTTCTATCACCTCCTAGCATTGAACTACGTGCCGTTGTTCGTATCCAAAAGTGNNTATTNTTGTANTCATTCACCGTCAAAGCTCAAGTAGTNNNTAGCACCACACCAGATTTCCAAAGCACAAAGCTCATGACTNGCCTTANCTACACACTCGATGAAATCNAAGGCCCTTTCNANGTTGATTACNNAGCAGTGACAGTACACAACTTCCTGGTGGGGAGCGTGTACCNTTNTTGTTCACNATCAAGCAGCTCGTGGCATCGCAGAGGATACTTTGNTCCCAAGTCGATCCCTTCAGCATTCACCCAAGGCCANGTTGGAAATAAATCTGATCGTAAAACNNNACNTCTGCAGATTATTATCAATAAAAATTCAGAAATCAATAAAATTCACTCTGAAAACGCAGAAAATCTAAGGTAAATTCATAACGACCTTTTGAATTTNNAGTGTCGAAGCTGTATNGAAGAAGAAGAAGAAGATTCCGAAGCTAACAAAGGAGAAGACGAGAGTGGATTGATTGNNNNNNNCTCCTCTNTGATGATATTCGTNCTTCTACACTCANAGAGATACACTGTTTATTATTGTTTTCAATATANGAGAGGAGNAGCNNCNNNACTATTTNTNNNTTTTTAGCNAATGNGAGAGCTCCACTTCATACCCCACTTTTACCAATCGTGCNNTTTCACAGCCACTCTCACNCATGTCGCGTTGAGCGCGTGTCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNTTGNATCCACGCACCTCGTTCTTAAAAACCNATCGCGTNTCNTATTTNTTTTGGTTTTCTTNTNTAAACCAAANTAAACCGAANCGAGATCAATTTGAATTTCNAAACCNATGTAAACCCATCGCTGTGAAAACAAAAAGNTCGGCCACAAAAATGGAGACGACACAACTTGGAAGAGAAATCAACNCGAAATAGCCAAGAGAATGTTCGNAAACCCCCTCNAANCCGATGCTTCCTTTGATTCCAACCTCCAAAAGCNCATCTGCTCAAATCCGAGGAGTATTACAAGGTACCTCTATAGAATATCAAGGCTATGACAATGCGGTTGCATTACCTGCTANAGGAAACAATGAGGAGCTAGCTAAGGAGAACAACAAGATCACACTTAGTGTAACAAAGAGTAATCNNGAGAGCGGTGAAGTGATTGGTGNTTTCNANAGTNTNCAACCTTCAGANACTGATTTGGGTGCTACGACTCCTAAGGATGTCAAGATTCAAGGAATCTGGTATTGCCAACTTGATGAANAAAACATNGANAATCTGTTTGTTTTCTTTTTCTGTAAATGTTGTTATGATTAAGGNATATTGATTCCAGTTCATAGTACATGTGGTACTCCATTTGGTAACACTTTGCACTTTTGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAAGATAACATATTTGTAGATCTGTATAATTGCTTGNAACAGCAANGATGATTTCTGGTTAANAAGTAAGAAGAGATTNATAATTAAGCAAACCATTTGCCTTATGACCCCATTGTTGTCACATCNACTNTNTCTTTTCACCTNCNNTGTCTCNAAAAACAGAGGACCNTTGCTCTGTTTCTTNANNCTGTNTTNTCTCCTACTCAAAGTTCAAATCNTTTGNNAAACCCAGTAATGGAAGATCAAAGNAGGTGAAAATGAGGAGAANNAGAGACGTTAACAAGGGNTCTTNTTCAAGGAGATGGTAAGNNNAACGGCGTCGTTTGGAACCAGCTCAAGCTTTGTTCTTCGNCTCGGACAAACTCTGTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.360067
PRIMER_LEFT_0_PENALTY=0.308722
PRIMER_RIGHT_0_PENALTY=0.051345
PRIMER_LEFT_0_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_0_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_0=464,20
PRIMER_RIGHT_0=1374,20
PRIMER_LEFT_0_TM=59.691
PRIMER_RIGHT_0_TM=60.051
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=911
PRIMER_PAIR_1_PENALTY=0.360067
PRIMER_LEFT_1_PENALTY=0.308722
PRIMER_RIGHT_1_PENALTY=0.051345
PRIMER_LEFT_1_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_1_SEQUENCE=GCTTTTGGAGGTTGGAATCA
PRIMER_LEFT_1=464,20
PRIMER_RIGHT_1=1383,20
PRIMER_LEFT_1_TM=59.691
PRIMER_RIGHT_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=920
PRIMER_PAIR_2_PENALTY=0.405577
PRIMER_LEFT_2_PENALTY=0.308722
PRIMER_RIGHT_2_PENALTY=0.096855
PRIMER_LEFT_2_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_2_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_2=464,20
PRIMER_RIGHT_2=1379,20
PRIMER_LEFT_2_TM=59.691
PRIMER_RIGHT_2_TM=59.903
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=916
PRIMER_PAIR_3_PENALTY=0.437109
PRIMER_LEFT_3_PENALTY=0.385764
PRIMER_RIGHT_3_PENALTY=0.051345
PRIMER_LEFT_3_SEQUENCE=TACGTGCCGTTGTTCGTATC
PRIMER_RIGHT_3_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_3=386,20
PRIMER_RIGHT_3=1374,20
PRIMER_LEFT_3_TM=59.614
PRIMER_RIGHT_3_TM=60.051
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=989
PRIMER_PAIR_4_PENALTY=0.437109
PRIMER_LEFT_4_PENALTY=0.385764
PRIMER_RIGHT_4_PENALTY=0.051345
PRIMER_LEFT_4_SEQUENCE=TACGTGCCGTTGTTCGTATC
PRIMER_RIGHT_4_SEQUENCE=GCTTTTGGAGGTTGGAATCA
PRIMER_LEFT_4=386,20
PRIMER_RIGHT_4=1383,20
PRIMER_LEFT_4_TM=59.614
PRIMER_RIGHT_4_TM=60.051
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=998
PRIMER_PAIR_5_PENALTY=0.441595
PRIMER_LEFT_5_PENALTY=0.390250
PRIMER_RIGHT_5_PENALTY=0.051345
PRIMER_LEFT_5_SEQUENCE=TCATTCACCGTCAAAGCTCA
PRIMER_RIGHT_5_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_5=427,20
PRIMER_RIGHT_5=1374,20
PRIMER_LEFT_5_TM=60.390
PRIMER_RIGHT_5_TM=60.051
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=948
PRIMER_PAIR_6_PENALTY=0.466501
PRIMER_LEFT_6_PENALTY=0.415156
PRIMER_RIGHT_6_PENALTY=0.051345
PRIMER_LEFT_6_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_6_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_6=471,20
PRIMER_RIGHT_6=1374,20
PRIMER_LEFT_6_TM=59.585
PRIMER_RIGHT_6_TM=60.051
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=904
PRIMER_PAIR_7_PENALTY=0.466501
PRIMER_LEFT_7_PENALTY=0.415156
PRIMER_RIGHT_7_PENALTY=0.051345
PRIMER_LEFT_7_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_7_SEQUENCE=GCTTTTGGAGGTTGGAATCA
PRIMER_LEFT_7=471,20
PRIMER_RIGHT_7=1383,20
PRIMER_LEFT_7_TM=59.585
PRIMER_RIGHT_7_TM=60.051
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=913
PRIMER_PAIR_8_PENALTY=0.487105
PRIMER_LEFT_8_PENALTY=0.390250
PRIMER_RIGHT_8_PENALTY=0.096855
PRIMER_LEFT_8_SEQUENCE=TCATTCACCGTCAAAGCTCA
PRIMER_RIGHT_8_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_8=427,20
PRIMER_RIGHT_8=1379,20
PRIMER_LEFT_8_TM=60.390
PRIMER_RIGHT_8_TM=59.903
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=953
PRIMER_PAIR_9_PENALTY=0.512012
PRIMER_LEFT_9_PENALTY=0.415156
PRIMER_RIGHT_9_PENALTY=0.096855
PRIMER_LEFT_9_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_9_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_9=471,20
PRIMER_RIGHT_9=1379,20
PRIMER_LEFT_9_TM=59.585
PRIMER_RIGHT_9_TM=59.903
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=909
PRIMER_PAIR_10_PENALTY=0.711580
PRIMER_LEFT_10_PENALTY=0.308722
PRIMER_RIGHT_10_PENALTY=0.402858
PRIMER_LEFT_10_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_10_SEQUENCE=AACCGCATTGTCATAGCCTT
PRIMER_LEFT_10=464,20
PRIMER_RIGHT_10=1452,20
PRIMER_LEFT_10_TM=59.691
PRIMER_RIGHT_10_TM=59.597
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=989
PRIMER_PAIR_11_PENALTY=0.736841
PRIMER_LEFT_11_PENALTY=0.308722
PRIMER_RIGHT_11_PENALTY=0.428120
PRIMER_LEFT_11_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_11_SEQUENCE=ATGCAACCGCATTGTCATAG
PRIMER_LEFT_11=464,20
PRIMER_RIGHT_11=1456,20
PRIMER_LEFT_11_TM=59.691
PRIMER_RIGHT_11_TM=59.572
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=5.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=993
PRIMER_PAIR_12_PENALTY=0.738695
PRIMER_LEFT_12_PENALTY=0.308722
PRIMER_RIGHT_12_PENALTY=0.429973
PRIMER_LEFT_12_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_12_SEQUENCE=TGGAGGTTGGAATCAAAGGA
PRIMER_LEFT_12=464,20
PRIMER_RIGHT_12=1378,20
PRIMER_LEFT_12_TM=59.691
PRIMER_RIGHT_12_TM=60.430
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=915
PRIMER_PAIR_13_PENALTY=0.767715
PRIMER_LEFT_13_PENALTY=0.308722
PRIMER_RIGHT_13_PENALTY=0.458994
PRIMER_LEFT_13_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_13_SEQUENCE=TGTCGTCTCCATTTTTGTGG
PRIMER_LEFT_13=464,20
PRIMER_RIGHT_13=1290,20
PRIMER_LEFT_13_TM=59.691
PRIMER_RIGHT_13_TM=59.541
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=827
PRIMER_PAIR_14_PENALTY=0.815738
PRIMER_LEFT_14_PENALTY=0.385764
PRIMER_RIGHT_14_PENALTY=0.429973
PRIMER_LEFT_14_SEQUENCE=TACGTGCCGTTGTTCGTATC
PRIMER_RIGHT_14_SEQUENCE=TGGAGGTTGGAATCAAAGGA
PRIMER_LEFT_14=386,20
PRIMER_RIGHT_14=1378,20
PRIMER_LEFT_14_TM=59.614
PRIMER_RIGHT_14_TM=60.430
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=5.3000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=993
PRIMER_PAIR_15_PENALTY=0.818015
PRIMER_LEFT_15_PENALTY=0.415156
PRIMER_RIGHT_15_PENALTY=0.402858
PRIMER_LEFT_15_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_15_SEQUENCE=AACCGCATTGTCATAGCCTT
PRIMER_LEFT_15=471,20
PRIMER_RIGHT_15=1452,20
PRIMER_LEFT_15_TM=59.585
PRIMER_RIGHT_15_TM=59.597
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=982
PRIMER_PAIR_16_PENALTY=0.820224
PRIMER_LEFT_16_PENALTY=0.390250
PRIMER_RIGHT_16_PENALTY=0.429973
PRIMER_LEFT_16_SEQUENCE=TCATTCACCGTCAAAGCTCA
PRIMER_RIGHT_16_SEQUENCE=TGGAGGTTGGAATCAAAGGA
PRIMER_LEFT_16=427,20
PRIMER_RIGHT_16=1378,20
PRIMER_LEFT_16_TM=60.390
PRIMER_RIGHT_16_TM=60.430
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=952
PRIMER_PAIR_17_PENALTY=0.843276
PRIMER_LEFT_17_PENALTY=0.415156
PRIMER_RIGHT_17_PENALTY=0.428120
PRIMER_LEFT_17_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_17_SEQUENCE=ATGCAACCGCATTGTCATAG
PRIMER_LEFT_17=471,20
PRIMER_RIGHT_17=1456,20
PRIMER_LEFT_17_TM=59.585
PRIMER_RIGHT_17_TM=59.572
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=5.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=986
PRIMER_PAIR_18_PENALTY=0.844758
PRIMER_LEFT_18_PENALTY=0.385764
PRIMER_RIGHT_18_PENALTY=0.458994
PRIMER_LEFT_18_SEQUENCE=TACGTGCCGTTGTTCGTATC
PRIMER_RIGHT_18_SEQUENCE=TGTCGTCTCCATTTTTGTGG
PRIMER_LEFT_18=386,20
PRIMER_RIGHT_18=1290,20
PRIMER_LEFT_18_TM=59.614
PRIMER_RIGHT_18_TM=59.541
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=5.3000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=905
PRIMER_PAIR_19_PENALTY=0.845130
PRIMER_LEFT_19_PENALTY=0.415156
PRIMER_RIGHT_19_PENALTY=0.429973
PRIMER_LEFT_19_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_19_SEQUENCE=TGGAGGTTGGAATCAAAGGA
PRIMER_LEFT_19=471,20
PRIMER_RIGHT_19=1378,20
PRIMER_LEFT_19_TM=59.585
PRIMER_RIGHT_19_TM=60.430
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=908
PRIMER_PAIR_20_PENALTY=0.849244
PRIMER_LEFT_20_PENALTY=0.390250
PRIMER_RIGHT_20_PENALTY=0.458994
PRIMER_LEFT_20_SEQUENCE=TCATTCACCGTCAAAGCTCA
PRIMER_RIGHT_20_SEQUENCE=TGTCGTCTCCATTTTTGTGG
PRIMER_LEFT_20=427,20
PRIMER_RIGHT_20=1290,20
PRIMER_LEFT_20_TM=60.390
PRIMER_RIGHT_20_TM=59.541
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=864
PRIMER_PAIR_21_PENALTY=0.852614
PRIMER_LEFT_21_PENALTY=0.801269
PRIMER_RIGHT_21_PENALTY=0.051345
PRIMER_LEFT_21_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_21_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_21=560,20
PRIMER_RIGHT_21=1374,20
PRIMER_LEFT_21_TM=60.801
PRIMER_RIGHT_21_TM=60.051
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=11.2000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=815
PRIMER_PAIR_22_PENALTY=0.852614
PRIMER_LEFT_22_PENALTY=0.801269
PRIMER_RIGHT_22_PENALTY=0.051345
PRIMER_LEFT_22_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_22_SEQUENCE=GCTTTTGGAGGTTGGAATCA
PRIMER_LEFT_22=560,20
PRIMER_RIGHT_22=1383,20
PRIMER_LEFT_22_TM=60.801
PRIMER_RIGHT_22_TM=60.051
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=11.2000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=824
PRIMER_PAIR_23_PENALTY=0.874150
PRIMER_LEFT_23_PENALTY=0.415156
PRIMER_RIGHT_23_PENALTY=0.458994
PRIMER_LEFT_23_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_23_SEQUENCE=TGTCGTCTCCATTTTTGTGG
PRIMER_LEFT_23=471,20
PRIMER_RIGHT_23=1290,20
PRIMER_LEFT_23_TM=59.585
PRIMER_RIGHT_23_TM=59.541
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=820
PRIMER_PAIR_24_PENALTY=0.898125
PRIMER_LEFT_24_PENALTY=0.801269
PRIMER_RIGHT_24_PENALTY=0.096855
PRIMER_LEFT_24_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_24_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_24=560,20
PRIMER_RIGHT_24=1379,20
PRIMER_LEFT_24_TM=60.801
PRIMER_RIGHT_24_TM=59.903
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=11.2000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=820
PRIMER_PAIR_25_PENALTY=1.073936
PRIMER_LEFT_25_PENALTY=0.801269
PRIMER_RIGHT_25_PENALTY=0.272667
PRIMER_LEFT_25_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_25_SEQUENCE=ACCAATCACTTCACCGCTCT
PRIMER_LEFT_25=560,20
PRIMER_RIGHT_25=1554,20
PRIMER_LEFT_25_TM=60.801
PRIMER_RIGHT_25_TM=59.727
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=11.2000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=995
PRIMER_PAIR_26_PENALTY=1.204128
PRIMER_LEFT_26_PENALTY=0.801269
PRIMER_RIGHT_26_PENALTY=0.402858
PRIMER_LEFT_26_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_26_SEQUENCE=AACCGCATTGTCATAGCCTT
PRIMER_LEFT_26=560,20
PRIMER_RIGHT_26=1452,20
PRIMER_LEFT_26_TM=60.801
PRIMER_RIGHT_26_TM=59.597
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=11.2000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=893
PRIMER_PAIR_27_PENALTY=1.210811
PRIMER_LEFT_27_PENALTY=1.159466
PRIMER_RIGHT_27_PENALTY=0.051345
PRIMER_LEFT_27_SEQUENCE=TCGATCCCTTCAGCATTCAC
PRIMER_RIGHT_27_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_27=646,20
PRIMER_RIGHT_27=1374,20
PRIMER_LEFT_27_TM=61.159
PRIMER_RIGHT_27_TM=60.051
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=729
PRIMER_PAIR_28_PENALTY=1.210811
PRIMER_LEFT_28_PENALTY=1.159466
PRIMER_RIGHT_28_PENALTY=0.051345
PRIMER_LEFT_28_SEQUENCE=TCGATCCCTTCAGCATTCAC
PRIMER_RIGHT_28_SEQUENCE=GCTTTTGGAGGTTGGAATCA
PRIMER_LEFT_28=646,20
PRIMER_RIGHT_28=1383,20
PRIMER_LEFT_28_TM=61.159
PRIMER_RIGHT_28_TM=60.051
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=738
PRIMER_PAIR_29_PENALTY=1.210811
PRIMER_LEFT_29_PENALTY=1.159466
PRIMER_RIGHT_29_PENALTY=0.051345
PRIMER_LEFT_29_SEQUENCE=GTCGATCCCTTCAGCATTCA
PRIMER_RIGHT_29_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_29=645,20
PRIMER_RIGHT_29=1374,20
PRIMER_LEFT_29_TM=61.159
PRIMER_RIGHT_29_TM=60.051
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=730
PRIMER_PAIR_30_PENALTY=1.210811
PRIMER_LEFT_30_PENALTY=1.159466
PRIMER_RIGHT_30_PENALTY=0.051345
PRIMER_LEFT_30_SEQUENCE=GTCGATCCCTTCAGCATTCA
PRIMER_RIGHT_30_SEQUENCE=GCTTTTGGAGGTTGGAATCA
PRIMER_LEFT_30=645,20
PRIMER_RIGHT_30=1383,20
PRIMER_LEFT_30_TM=61.159
PRIMER_RIGHT_30_TM=60.051
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=739
PRIMER_PAIR_31_PENALTY=1.229389
PRIMER_LEFT_31_PENALTY=0.801269
PRIMER_RIGHT_31_PENALTY=0.428120
PRIMER_LEFT_31_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_31_SEQUENCE=ATGCAACCGCATTGTCATAG
PRIMER_LEFT_31=560,20
PRIMER_RIGHT_31=1456,20
PRIMER_LEFT_31_TM=60.801
PRIMER_RIGHT_31_TM=59.572
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=11.2000
PRIMER_RIGHT_31_END_STABILITY=5.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=897
PRIMER_PAIR_32_PENALTY=1.231243
PRIMER_LEFT_32_PENALTY=0.801269
PRIMER_RIGHT_32_PENALTY=0.429973
PRIMER_LEFT_32_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_32_SEQUENCE=TGGAGGTTGGAATCAAAGGA
PRIMER_LEFT_32=560,20
PRIMER_RIGHT_32=1378,20
PRIMER_LEFT_32_TM=60.801
PRIMER_RIGHT_32_TM=60.430
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=11.2000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=819
PRIMER_PAIR_33_PENALTY=1.256321
PRIMER_LEFT_33_PENALTY=1.159466
PRIMER_RIGHT_33_PENALTY=0.096855
PRIMER_LEFT_33_SEQUENCE=TCGATCCCTTCAGCATTCAC
PRIMER_RIGHT_33_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_33=646,20
PRIMER_RIGHT_33=1379,20
PRIMER_LEFT_33_TM=61.159
PRIMER_RIGHT_33_TM=59.903
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=734
PRIMER_PAIR_34_PENALTY=1.256321
PRIMER_LEFT_34_PENALTY=1.159466
PRIMER_RIGHT_34_PENALTY=0.096855
PRIMER_LEFT_34_SEQUENCE=GTCGATCCCTTCAGCATTCA
PRIMER_RIGHT_34_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_34=645,20
PRIMER_RIGHT_34=1379,20
PRIMER_LEFT_34_TM=61.159
PRIMER_RIGHT_34_TM=59.903
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=735
PRIMER_PAIR_35_PENALTY=1.260263
PRIMER_LEFT_35_PENALTY=0.801269
PRIMER_RIGHT_35_PENALTY=0.458994
PRIMER_LEFT_35_SEQUENCE=TACACAACTTCCTGGTGGGG
PRIMER_RIGHT_35_SEQUENCE=TGTCGTCTCCATTTTTGTGG
PRIMER_LEFT_35=560,20
PRIMER_RIGHT_35=1290,20
PRIMER_LEFT_35_TM=60.801
PRIMER_RIGHT_35_TM=59.541
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=11.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=731
PRIMER_PAIR_36_PENALTY=1.306267
PRIMER_LEFT_36_PENALTY=1.254922
PRIMER_RIGHT_36_PENALTY=0.051345
PRIMER_LEFT_36_SEQUENCE=CCAGATTTCCAAAGCACAAA
PRIMER_RIGHT_36_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_36=466,20
PRIMER_RIGHT_36=1374,20
PRIMER_LEFT_36_TM=58.745
PRIMER_RIGHT_36_TM=60.051
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=909
PRIMER_PAIR_37_PENALTY=1.308425
PRIMER_LEFT_37_PENALTY=1.257080
PRIMER_RIGHT_37_PENALTY=0.051345
PRIMER_LEFT_37_SEQUENCE=AAGTCGATCCCTTCAGCATT
PRIMER_RIGHT_37_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_37=643,20
PRIMER_RIGHT_37=1374,20
PRIMER_LEFT_37_TM=58.743
PRIMER_RIGHT_37_TM=60.051
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=732
PRIMER_PAIR_38_PENALTY=1.308425
PRIMER_LEFT_38_PENALTY=1.257080
PRIMER_RIGHT_38_PENALTY=0.051345
PRIMER_LEFT_38_SEQUENCE=AAGTCGATCCCTTCAGCATT
PRIMER_RIGHT_38_SEQUENCE=GCTTTTGGAGGTTGGAATCA
PRIMER_LEFT_38=643,20
PRIMER_RIGHT_38=1383,20
PRIMER_LEFT_38_TM=58.743
PRIMER_RIGHT_38_TM=60.051
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.4000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=741
PRIMER_PAIR_39_PENALTY=1.318839
PRIMER_LEFT_39_PENALTY=1.267494
PRIMER_RIGHT_39_PENALTY=0.051345
PRIMER_LEFT_39_SEQUENCE=CCAGATTTCCAAAGCACAAAG
PRIMER_RIGHT_39_SEQUENCE=GGTTGGAATCAAAGGAAGCA
PRIMER_LEFT_39=466,21
PRIMER_RIGHT_39=1374,20
PRIMER_LEFT_39_TM=59.733
PRIMER_RIGHT_39_TM=60.051
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=909
PRIMER_PAIR_40_PENALTY=1.351777
PRIMER_LEFT_40_PENALTY=1.254922
PRIMER_RIGHT_40_PENALTY=0.096855
PRIMER_LEFT_40_SEQUENCE=CCAGATTTCCAAAGCACAAA
PRIMER_RIGHT_40_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_40=466,20
PRIMER_RIGHT_40=1379,20
PRIMER_LEFT_40_TM=58.745
PRIMER_RIGHT_40_TM=59.903
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=914
PRIMER_PAIR_41_PENALTY=1.352433
PRIMER_LEFT_41_PENALTY=0.308722
PRIMER_RIGHT_41_PENALTY=1.043712
PRIMER_LEFT_41_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_41_SEQUENCE=GGAGGTTGGAATCAAAGGAA
PRIMER_LEFT_41=464,20
PRIMER_RIGHT_41=1377,20
PRIMER_LEFT_41_TM=59.691
PRIMER_RIGHT_41_TM=58.956
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=914
PRIMER_PAIR_42_PENALTY=1.353936
PRIMER_LEFT_42_PENALTY=1.257080
PRIMER_RIGHT_42_PENALTY=0.096855
PRIMER_LEFT_42_SEQUENCE=AAGTCGATCCCTTCAGCATT
PRIMER_RIGHT_42_SEQUENCE=TTGGAGGTTGGAATCAAAGG
PRIMER_LEFT_42=643,20
PRIMER_RIGHT_42=1379,20
PRIMER_LEFT_42_TM=58.743
PRIMER_RIGHT_42_TM=59.903
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=737
PRIMER_PAIR_43_PENALTY=1.382622
PRIMER_LEFT_43_PENALTY=0.308722
PRIMER_RIGHT_43_PENALTY=1.073900
PRIMER_LEFT_43_SEQUENCE=CACCAGATTTCCAAAGCACA
PRIMER_RIGHT_43_SEQUENCE=GGAGGTTGGAATCAAAGGAAG
PRIMER_LEFT_43=464,20
PRIMER_RIGHT_43=1377,21
PRIMER_LEFT_43_TM=59.691
PRIMER_RIGHT_43_TM=59.926
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=914
PRIMER_PAIR_44_PENALTY=1.432133
PRIMER_LEFT_44_PENALTY=1.159466
PRIMER_RIGHT_44_PENALTY=0.272667
PRIMER_LEFT_44_SEQUENCE=TCGATCCCTTCAGCATTCAC
PRIMER_RIGHT_44_SEQUENCE=ACCAATCACTTCACCGCTCT
PRIMER_LEFT_44=646,20
PRIMER_RIGHT_44=1554,20
PRIMER_LEFT_44_TM=61.159
PRIMER_RIGHT_44_TM=59.727
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=909
PRIMER_PAIR_45_PENALTY=1.432133
PRIMER_LEFT_45_PENALTY=1.159466
PRIMER_RIGHT_45_PENALTY=0.272667
PRIMER_LEFT_45_SEQUENCE=GTCGATCCCTTCAGCATTCA
PRIMER_RIGHT_45_SEQUENCE=ACCAATCACTTCACCGCTCT
PRIMER_LEFT_45=645,20
PRIMER_RIGHT_45=1554,20
PRIMER_LEFT_45_TM=61.159
PRIMER_RIGHT_45_TM=59.727
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=910
PRIMER_PAIR_46_PENALTY=1.433962
PRIMER_LEFT_46_PENALTY=0.390250
PRIMER_RIGHT_46_PENALTY=1.043712
PRIMER_LEFT_46_SEQUENCE=TCATTCACCGTCAAAGCTCA
PRIMER_RIGHT_46_SEQUENCE=GGAGGTTGGAATCAAAGGAA
PRIMER_LEFT_46=427,20
PRIMER_RIGHT_46=1377,20
PRIMER_LEFT_46_TM=60.390
PRIMER_RIGHT_46_TM=58.956
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=951
PRIMER_PAIR_47_PENALTY=1.458868
PRIMER_LEFT_47_PENALTY=0.415156
PRIMER_RIGHT_47_PENALTY=1.043712
PRIMER_LEFT_47_SEQUENCE=TTTCCAAAGCACAAAGCTCA
PRIMER_RIGHT_47_SEQUENCE=GGAGGTTGGAATCAAAGGAA
PRIMER_LEFT_47=471,20
PRIMER_RIGHT_47=1377,20
PRIMER_LEFT_47_TM=59.585
PRIMER_RIGHT_47_TM=58.956
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=907
PRIMER_PAIR_48_PENALTY=1.459665
PRIMER_LEFT_48_PENALTY=0.385764
PRIMER_RIGHT_48_PENALTY=1.073900
PRIMER_LEFT_48_SEQUENCE=TACGTGCCGTTGTTCGTATC
PRIMER_RIGHT_48_SEQUENCE=GGAGGTTGGAATCAAAGGAAG
PRIMER_LEFT_48=386,20
PRIMER_RIGHT_48=1377,21
PRIMER_LEFT_48_TM=59.614
PRIMER_RIGHT_48_TM=59.926
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.3000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=992
PRIMER_PAIR_49_PENALTY=1.464151
PRIMER_LEFT_49_PENALTY=0.390250
PRIMER_RIGHT_49_PENALTY=1.073900
PRIMER_LEFT_49_SEQUENCE=TCATTCACCGTCAAAGCTCA
PRIMER_RIGHT_49_SEQUENCE=GGAGGTTGGAATCAAAGGAAG
PRIMER_LEFT_49=427,20
PRIMER_RIGHT_49=1377,21
PRIMER_LEFT_49_TM=60.390
PRIMER_RIGHT_49_TM=59.926
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=951
=
SEQUENCE_ID=MAGIC.105_4_1539793_1539806_po-0_rsch-4_79772_23981_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=CCTGTCCAGCTTCAACAGCTAAATACAAGGGAGAAANCCCATCGTTGTTTGNTACAAAAGACAAACTTTGCTCTGCANANACCAAACAGGAAGCCACNTTCANATGTTTTCNTTTTAANGNNACATGTAAAGCATTGTCTTGGTGNNTGTCCTTGGCAAAGTATATCTTTTTGGCAACACCAGGCTTATTACAAGATATATCTTTGATAAAACTAACCAGAGCCTCAACNACAGCGAGATGGCCTGCACCTGCTGCAACATGGAGAGCAACTTCCCCCATGGAATTTGACTTCATAAGCAGACCTGGGTACGCATTGAGTATATAACACACTAAGTCGGTGTGACCCGCGGCTGCAGCAAGATGAAGCATTGTATTNCCATGATCGTTCACTGAGCTTAAAGCAATGTTAACATCAANTTTCATATCTCTCANGTAAGTTTCATCACCTGCTCTTACAGCGGCGATGGTCTTTGGACCCATTGGCACAGTTTCACTTGTGTCATTGAAGAGANTTGACAAATCGAGAGAGTGCGTACGCCCGGTTTCGTCTGCATAAAACATCCNAGAGCTTGCGTCTTTCTTCNGACGGTCAAANTCCAGATCAGACNTTCCCATATTGATAATTTGGCNNCNAAACAATAAGGAANAANNNNNNNNNNAANANCTACATACGATGAATTGATGAAGTAGAAACAAATATGCTTNNATCATTGNGACTCAAAGNTTTTATATTTAATGATTGAACCGCAAGGTAATTGTATATCATGCACTAACATAGTTGCCTGAANCTTCANANAACAGAANCTCAAAGAGNAAGAAAACAATGAAAACAGAGTAAAAAGTAAGCTTGAGGTTTCTCACCTTATTCGTCAGCCGAAGCAACGACTTGATGCTGGAGGGCTCAGGGAAGGCTTTNCCCAACTTCAATATTTCCAAATGCTTGCAGAACCAACTCCACAATGCTTGAGATATAGATTGGTAAGTGGTATCTACAGNAAANAGAAGTCTTNAGNTTTTANNANTGTCCATCANGGAAGAAGAAATNGTCANNNNNNTAAAAGGAANCANACGCGNACCTTCTTTNTTTTTNCTTTCNCATCAGANNAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTATANTNTNTAANGGNTTTNTCAAGTCTACCTTATTTGTNNTATTACANAGAAAACAAGNCATGNTCCAAAAGAAGAAAGCANTNNGTGGGATACTNNNNNNNNNNNNTATATAGAAANNCAACGACTTNTGGGTTTNTTCAACTTCTAANGTCAACTATCTNNNNNNNNNTCAACATNTCTCNAAGCTACTATCCAAAAATTCCATTACTCTNGAAAGTCTTGAAGAAAANCGGACGCAATAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTCAACGAATTAGAAAAACAANCGGCGACAAGACCGATCTNTTGTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTNAGAGTAGTGCNCCGTCCTCAATNCTCCACNTCTGCNTNNCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCCGTCTCCGNCNATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCNNNNAAGGTNTGTAATATTNGGCCTATAANAGNTTACANATGGCCCNNNNNNNNNNNNNNNTTNNTNGTNTNTACAATGAAAAGTCGAACTCAACGTNGTGTTCACANTTGGGCCTATAAAGNTCAACAAAANNTNGGCNCAGTTTAATATAGNCTTTTCATCTCCGCGACGCNGTTTCGTAAGAGTGNNNANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=5.829829
PRIMER_LEFT_0_PENALTY=1.795587
PRIMER_RIGHT_0_PENALTY=4.034241
PRIMER_LEFT_0_SEQUENCE=CAACGACTTGATGCTGGAGG
PRIMER_RIGHT_0_SEQUENCE=ACGTTGAGTTCGACTTTTCA
PRIMER_LEFT_0=880,20
PRIMER_RIGHT_0=1874,20
PRIMER_LEFT_0_TM=61.796
PRIMER_RIGHT_0_TM=55.966
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=995
PRIMER_PAIR_1_PENALTY=6.564332
PRIMER_LEFT_1_PENALTY=2.530091
PRIMER_RIGHT_1_PENALTY=4.034241
PRIMER_LEFT_1_SEQUENCE=AACGACTTGATGCTGGAGGG
PRIMER_RIGHT_1_SEQUENCE=ACGTTGAGTTCGACTTTTCA
PRIMER_LEFT_1=881,20
PRIMER_RIGHT_1=1874,20
PRIMER_LEFT_1_TM=62.530
PRIMER_RIGHT_1_TM=55.966
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=994
=
SEQUENCE_ID=MAGIC.105_3_9613022_9613342_kn-0_oy-0_596402_12524_TRUE
SEQUENCE_TARGET=950,420
SEQUENCE_TEMPLATE=CNTCTTCNGGATTTATTTTNCCCTCACAAGCTTCACACCAATANNTCATGCTACTTGTCTTCTTCCCATAANAAAGAGGGAGTATGTGTTTATCATNCNTATACCTNACTTNTTGNGGTAAAGTAGCACATTTNAAACACAAAGCANANTCACACNCNTNCTCAATGCAATTGAAGGTTTCCTTGGTGTAATAACCTCGCGCTTCGGCGCAAACCGAACANTTTCTTTNCTCNTNTNGTNTGGATGTTAGGAATAAAGGATGCNTATGACTTGGATGAAGTAAAGGCTCCGAAATTNTGGCGCACTGCACATGTAGCCTAAAATCGCAATTTCTTTCACTGCACTNATAGAANAAACCAGCTATACACAACGAAGGACAAGCTGAACANNTATCACGATCAGAATTANNNNNNNNNNCGACAGNTCTGAGAGGGTTATCTGTNACTAAAGTGAGTNGATGNGGATGTATCGGGTGATNTNANCTGCGAGAAAGNTTTGCACATTCTTCNTGTAGAATGAAATCACATTGCAANCAAGAGTAGGAGTTACCAAAATAAATNGGCATGATGCATGCTTGACANAGCTTATTCTCGTCGTAATCTTTGNNNGTGTTCTCATNAAGTGTCAAATGATGATNTNCATGACTGAAATNTTGTATGANTCCATCGCTTATNGTCANGAATGGCTCAAGNTCTTCCACTTCTTCTTCNNACTCTCCTTCACGTNCTATACCATCCCAAACATTTGTTTGAGTGGCNCATTTTGAATGNACCGCATAAANACAACCNTTCTTGATGCAATGATANCCCCCGTANTCATTGTTAATCTTCCGGCGGCAAANACCACAAATNCNATCTCCTTTTGGGAAAGAATGGGTAAAANAGATANAATGGNNGTGNCGAGAAATCCGTATGACATGAGGTAAGCTNAAACANCTTTGATGNACGATAAAGTNGCAAGGNGGNCATATATAGAAAANCAANTTTGAATGTTTNAAGGCNCANAGGCTGCAAGTTNNGGNATCCNGACTTGGAAANANAGCAAGTGTATGCNCATGCCACTTGGGTTGGTCGATAGACAAGACTNGTGATTTCTTNGCACATGTAAATTTGAGCCCAAAATCGNAAGNAGAACAAAAATAAAACATACTTGTGGTATCGTCATGGAGAANTTCGTGACAGCAATAGCATTGCNNNANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17765994_17766517_oy-0_edi-0_46917_8532_TRUE
SEQUENCE_TARGET=950,623
SEQUENCE_TEMPLATE=GTGTTATTAATGACTTATTGCCGACATATTNNAAAAAAAACTCTTCATAATATATACTCCATGTGTATTAGTGTATGTATANCATTTCATCTATCATTATGCATANNAGCAAACATGTAAAATAATAAGTATATGTGACAGTTTACAAACATCATTTGTCTGATTAAATCATTAAAAGATAACAAAATTGTTCTGACCATTTCGTNNAAAAANAATNAAGTAGTATACAATAGAAACAAATAATTTAAGAAGTCGTTTATTCAGAAATGATTCTGGTAATCGCACGAAGCTTATTATTCATCATCGTTATCTTTCATACTNTAAGATAATAATTAAGAAGATCCTAGTAAAATTTGGTCTTTGAATCTGACCTCTTTTCTACGTTCTATATTTTGTCATGTAATGCGATTATTTTGATATGATCATGCGTCATAGAACACACGCTCCATAAGACAAAATGTGATAACATATTAAATTCGATAACAANAAATAAGACAGAAACAAAACTAATCATGACGACGTCTAGTTACAGGAAGATCGCCTAAATGACTAAATTAAATAAATTATCGGGTCAATACAATTACTAATTTGTCTTCTCGTTCTAACTTTGAAATNTTTAGATATTGGGTCTTTGTTGAAAAAATATGATTTAATCATGTCATGATTTTTCTAATCTTGACACCTCGAGTTTTAAATACAACACTAGGACTATGTAATTATTAAATAATACATGTAGTTAATAAATACATGTGTCGTCGTGTTCATGAGTTTGCCTCACCTTTCCTCTTAGGAGACTGGTCTGGCCGTCGGGGACCAATGCTCTGAGATACAAAATTATTTTTNTACTAATAATTTATAGTATAATAATACGTATTCTTTGTATGCAATTCAAATTGTTTTCATGCATCCCTCTCTTTAAATGAATTGCAGCTTGTCCAAATGGTTAAAAGTTCTCGTTAGTTGGAATCAAATATCTATATCTTCAACATTCTCAGAACTANATATATTCTAAAGTTTCAATAATTTTTGTTCCAAACAACAAAATTGACTCTTTAAATAAAGTCAGGAAGATTTTGGTAATGGCGATTGCAATCTTTTTAGTCAAAAGACACCAGCAAAATGATCAAAAGGCGAATTTAAATAACTTCATTTGTAACTTCTGACGCCCCGACTATTCTAGCTGTTACAAGTGACCAAAATGGCTAACAATGTACACGTTNAACTTTTGATTTTCACACTACCATATTTTATAAAAATCATGATGTTCTAATTGATTATATAAATAAAATGCTTTACATATGCATGTAGTTCACNTTTTTTTTTTTTNGTTTGCAATTGTTTCTTAAATAAATAAATAAAAAACNTAATTTAAAACAAATCAGAAGAAAAAAAAATTGCAAACCAAAGTAATCCAAATAAGCTAGTATTTTAATCGGATTGTGAGTACTTTCTCCACTTCTCCTTCGAGGCCACGCTTATATATTAATTTGTACAATATGGGTAACCTAACTTGGTTGGAAAATNGAAAGTGCNAAAAAAAACAAAATATGTATGGCTAAAACCAATAAAACGTCCCACGTCGACTTTCAAGTTAAGTCCCTCAACAATATTATTTCTGTTTGACGAATCACATTCCACCACCACCATGATTACCAATCTTCTTCTCCCACCCGTTTCTAATTCAGGCCATTCACAATATTTGATACAGAATAATGAAACTTAATGTTGAACTATTCCGCCATTTTACTGTTTCAATTAATTAAATATTTATCTAAACGAGGTGCTGTTAGTAAAAAAAGTTCCAATATTTTTCTAGCTAATTCTATAAAAAGACACTTTGTACTGTAAGAGCAGAGACGATNAAAACAGAAAATGTAACGATAATTTGTTACGTCCTGTAATGAATTGTTTAAATTCGAGTAATGATATTTATGCATTTTCTACATATATGCTACTAGTAATCAATAAAAGCATAGTATTATCAAAACTAGAATGCGGTGGAAATAAATAGATAGAAATCATTGGATAAATAAGTCCCAACACAATCTGGCCAGATCAAGAGAACCGAACCTAAAACAAGAAAGTGGAGATCACAATCCGAAACCCTAAATAATAATACAAGAAAGGGGAATATAAAAAGAAATTAGAAGAAATTAATNAAAAACCCAATGTGTAGTGTGAGGAAGTTGAAGATTGGTAGGCAAGCTGACATGTTACCCATATCTACCTTCTTCAAGCAAAGCATGGAGTGGGAGACAATTCATGTGGTCTCTTATTTAATTAAAGTTGCCTCTTAATTNCTCTCTATTGACAACTTATTATGATACTCCTACTTAGATAAGTTAAATGTATCATTTGATTATAATCTAATTTATTTNAAGGAAGTCTTCAGTTCTGTTAGTTATATTTAACGCTTCAGTGGTATATTACAAGAATATCTGTACTCGAGTAAATATTTTAAAATATCNATCACAGAACATTGGATCTTTGAATGTGGGNNNNNNNGGATATAGAATCGTTTCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.320416
PRIMER_LEFT_0_PENALTY=0.232299
PRIMER_RIGHT_0_PENALTY=0.088117
PRIMER_LEFT_0_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_0_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_0=764,20
PRIMER_RIGHT_0=1654,20
PRIMER_LEFT_0_TM=60.232
PRIMER_RIGHT_0_TM=60.088
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=891
PRIMER_PAIR_1_PENALTY=0.567633
PRIMER_LEFT_1_PENALTY=0.232299
PRIMER_RIGHT_1_PENALTY=0.335333
PRIMER_LEFT_1_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_1_SEQUENCE=TAGAAACGGGTGGGAGAAGA
PRIMER_LEFT_1=764,20
PRIMER_RIGHT_1=1678,20
PRIMER_LEFT_1_TM=60.232
PRIMER_RIGHT_1_TM=59.665
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=915
PRIMER_PAIR_2_PENALTY=0.663356
PRIMER_LEFT_2_PENALTY=0.232299
PRIMER_RIGHT_2_PENALTY=0.431057
PRIMER_LEFT_2_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_2_SEQUENCE=GCCTGAATTAGAAACGGGTG
PRIMER_LEFT_2=764,20
PRIMER_RIGHT_2=1686,20
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=59.569
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=923
PRIMER_PAIR_3_PENALTY=0.795443
PRIMER_LEFT_3_PENALTY=0.707326
PRIMER_RIGHT_3_PENALTY=0.088117
PRIMER_LEFT_3_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_3_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_3=756,20
PRIMER_RIGHT_3=1654,20
PRIMER_LEFT_3_TM=59.293
PRIMER_RIGHT_3_TM=60.088
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=899
PRIMER_PAIR_4_PENALTY=0.795443
PRIMER_LEFT_4_PENALTY=0.707326
PRIMER_RIGHT_4_PENALTY=0.088117
PRIMER_LEFT_4_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_4_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_4=755,20
PRIMER_RIGHT_4=1654,20
PRIMER_LEFT_4_TM=59.293
PRIMER_RIGHT_4_TM=60.088
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=900
PRIMER_PAIR_5_PENALTY=0.807818
PRIMER_LEFT_5_PENALTY=0.232299
PRIMER_RIGHT_5_PENALTY=0.575519
PRIMER_LEFT_5_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_5_SEQUENCE=CTGAATTAGAAACGGGTGGG
PRIMER_LEFT_5=764,20
PRIMER_RIGHT_5=1684,20
PRIMER_LEFT_5_TM=60.232
PRIMER_RIGHT_5_TM=59.424
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=921
PRIMER_PAIR_6_PENALTY=0.876861
PRIMER_LEFT_6_PENALTY=0.232299
PRIMER_RIGHT_6_PENALTY=0.644562
PRIMER_LEFT_6_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_6_SEQUENCE=GGTGGTGGAATGTGATTCGT
PRIMER_LEFT_6=764,20
PRIMER_RIGHT_6=1641,20
PRIMER_LEFT_6_TM=60.232
PRIMER_RIGHT_6_TM=60.645
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=878
PRIMER_PAIR_7_PENALTY=0.884424
PRIMER_LEFT_7_PENALTY=0.232299
PRIMER_RIGHT_7_PENALTY=0.652125
PRIMER_LEFT_7_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_7_SEQUENCE=GGTGGAATGTGATTCGTCAA
PRIMER_LEFT_7=764,20
PRIMER_RIGHT_7=1638,20
PRIMER_LEFT_7_TM=60.232
PRIMER_RIGHT_7_TM=59.348
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=875
PRIMER_PAIR_8_PENALTY=0.945325
PRIMER_LEFT_8_PENALTY=0.857208
PRIMER_RIGHT_8_PENALTY=0.088117
PRIMER_LEFT_8_SEQUENCE=GTCGTGTTCATGAGTTTGCC
PRIMER_RIGHT_8_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_8=754,20
PRIMER_RIGHT_8=1654,20
PRIMER_LEFT_8_TM=59.143
PRIMER_RIGHT_8_TM=60.088
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.0000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=901
PRIMER_PAIR_9_PENALTY=1.008517
PRIMER_LEFT_9_PENALTY=0.707326
PRIMER_RIGHT_9_PENALTY=0.301190
PRIMER_LEFT_9_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_9_SEQUENCE=TGAATTAGAAACGGGTGGGA
PRIMER_LEFT_9=756,20
PRIMER_RIGHT_9=1683,20
PRIMER_LEFT_9_TM=59.293
PRIMER_RIGHT_9_TM=60.301
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=928
PRIMER_PAIR_10_PENALTY=1.008517
PRIMER_LEFT_10_PENALTY=0.707326
PRIMER_RIGHT_10_PENALTY=0.301190
PRIMER_LEFT_10_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_10_SEQUENCE=TGAATTAGAAACGGGTGGGA
PRIMER_LEFT_10=755,20
PRIMER_RIGHT_10=1683,20
PRIMER_LEFT_10_TM=59.293
PRIMER_RIGHT_10_TM=60.301
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=929
PRIMER_PAIR_11_PENALTY=1.042660
PRIMER_LEFT_11_PENALTY=0.707326
PRIMER_RIGHT_11_PENALTY=0.335333
PRIMER_LEFT_11_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_11_SEQUENCE=TAGAAACGGGTGGGAGAAGA
PRIMER_LEFT_11=756,20
PRIMER_RIGHT_11=1678,20
PRIMER_LEFT_11_TM=59.293
PRIMER_RIGHT_11_TM=59.665
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=923
PRIMER_PAIR_12_PENALTY=1.042660
PRIMER_LEFT_12_PENALTY=0.707326
PRIMER_RIGHT_12_PENALTY=0.335333
PRIMER_LEFT_12_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_12_SEQUENCE=TAGAAACGGGTGGGAGAAGA
PRIMER_LEFT_12=755,20
PRIMER_RIGHT_12=1678,20
PRIMER_LEFT_12_TM=59.293
PRIMER_RIGHT_12_TM=59.665
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=924
PRIMER_PAIR_13_PENALTY=1.054227
PRIMER_LEFT_13_PENALTY=0.232299
PRIMER_RIGHT_13_PENALTY=0.821928
PRIMER_LEFT_13_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_13_SEQUENCE=GGGTGGGAGAAGAAGATTGG
PRIMER_LEFT_13=764,20
PRIMER_RIGHT_13=1671,20
PRIMER_LEFT_13_TM=60.232
PRIMER_RIGHT_13_TM=60.822
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=908
PRIMER_PAIR_14_PENALTY=1.074943
PRIMER_LEFT_14_PENALTY=0.232299
PRIMER_RIGHT_14_PENALTY=0.842644
PRIMER_LEFT_14_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_14_SEQUENCE=TGAAACAGTAAAATGGCGGA
PRIMER_LEFT_14=764,20
PRIMER_RIGHT_14=1753,20
PRIMER_LEFT_14_TM=60.232
PRIMER_RIGHT_14_TM=59.157
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=11.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=990
PRIMER_PAIR_15_PENALTY=1.083963
PRIMER_LEFT_15_PENALTY=0.707326
PRIMER_RIGHT_15_PENALTY=0.376636
PRIMER_LEFT_15_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_15_SEQUENCE=TAATCATGGTGGTGGTGGAA
PRIMER_LEFT_15=756,20
PRIMER_RIGHT_15=1651,20
PRIMER_LEFT_15_TM=59.293
PRIMER_RIGHT_15_TM=59.623
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=896
PRIMER_PAIR_16_PENALTY=1.083963
PRIMER_LEFT_16_PENALTY=0.707326
PRIMER_RIGHT_16_PENALTY=0.376636
PRIMER_LEFT_16_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_16_SEQUENCE=TAATCATGGTGGTGGTGGAA
PRIMER_LEFT_16=755,20
PRIMER_RIGHT_16=1651,20
PRIMER_LEFT_16_TM=59.293
PRIMER_RIGHT_16_TM=59.623
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=897
PRIMER_PAIR_17_PENALTY=1.135014
PRIMER_LEFT_17_PENALTY=0.232299
PRIMER_RIGHT_17_PENALTY=0.902715
PRIMER_LEFT_17_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_17_SEQUENCE=GGTAATCATGGTGGTGGTGG
PRIMER_LEFT_17=764,20
PRIMER_RIGHT_17=1653,20
PRIMER_LEFT_17_TM=60.232
PRIMER_RIGHT_17_TM=60.903
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=890
PRIMER_PAIR_18_PENALTY=1.138384
PRIMER_LEFT_18_PENALTY=0.707326
PRIMER_RIGHT_18_PENALTY=0.431057
PRIMER_LEFT_18_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_18_SEQUENCE=GCCTGAATTAGAAACGGGTG
PRIMER_LEFT_18=756,20
PRIMER_RIGHT_18=1686,20
PRIMER_LEFT_18_TM=59.293
PRIMER_RIGHT_18_TM=59.569
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=931
PRIMER_PAIR_19_PENALTY=1.138384
PRIMER_LEFT_19_PENALTY=0.707326
PRIMER_RIGHT_19_PENALTY=0.431057
PRIMER_LEFT_19_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_19_SEQUENCE=GCCTGAATTAGAAACGGGTG
PRIMER_LEFT_19=755,20
PRIMER_RIGHT_19=1686,20
PRIMER_LEFT_19_TM=59.293
PRIMER_RIGHT_19_TM=59.569
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=932
PRIMER_PAIR_20_PENALTY=1.154260
PRIMER_LEFT_20_PENALTY=1.066143
PRIMER_RIGHT_20_PENALTY=0.088117
PRIMER_LEFT_20_SEQUENCE=TTGTTTTCATGCATCCCTCTC
PRIMER_RIGHT_20_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_20=893,21
PRIMER_RIGHT_20=1654,20
PRIMER_LEFT_20_TM=60.066
PRIMER_RIGHT_20_TM=60.088
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=762
PRIMER_PAIR_21_PENALTY=1.158398
PRIMER_LEFT_21_PENALTY=0.857208
PRIMER_RIGHT_21_PENALTY=0.301190
PRIMER_LEFT_21_SEQUENCE=GTCGTGTTCATGAGTTTGCC
PRIMER_RIGHT_21_SEQUENCE=TGAATTAGAAACGGGTGGGA
PRIMER_LEFT_21=754,20
PRIMER_RIGHT_21=1683,20
PRIMER_LEFT_21_TM=59.143
PRIMER_RIGHT_21_TM=60.301
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=10.0000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=930
PRIMER_PAIR_22_PENALTY=1.169365
PRIMER_LEFT_22_PENALTY=0.232299
PRIMER_RIGHT_22_PENALTY=0.937066
PRIMER_LEFT_22_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_22_SEQUENCE=GTAATCATGGTGGTGGTGGA
PRIMER_LEFT_22=764,20
PRIMER_RIGHT_22=1652,20
PRIMER_LEFT_22_TM=60.232
PRIMER_RIGHT_22_TM=59.063
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=889
PRIMER_PAIR_23_PENALTY=1.179356
PRIMER_LEFT_23_PENALTY=1.091240
PRIMER_RIGHT_23_PENALTY=0.088117
PRIMER_LEFT_23_SEQUENCE=TTTGCCTCACCTTTCCTCTT
PRIMER_RIGHT_23_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_23=768,20
PRIMER_RIGHT_23=1654,20
PRIMER_LEFT_23_TM=58.909
PRIMER_RIGHT_23_TM=60.088
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=887
PRIMER_PAIR_24_PENALTY=1.189051
PRIMER_LEFT_24_PENALTY=0.232299
PRIMER_RIGHT_24_PENALTY=0.956752
PRIMER_LEFT_24_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_24_SEQUENCE=ATGGCCTGAATTAGAAACGG
PRIMER_LEFT_24=764,20
PRIMER_RIGHT_24=1689,20
PRIMER_LEFT_24_TM=60.232
PRIMER_RIGHT_24_TM=59.043
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=9.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=926
PRIMER_PAIR_25_PENALTY=1.192542
PRIMER_LEFT_25_PENALTY=0.857208
PRIMER_RIGHT_25_PENALTY=0.335333
PRIMER_LEFT_25_SEQUENCE=GTCGTGTTCATGAGTTTGCC
PRIMER_RIGHT_25_SEQUENCE=TAGAAACGGGTGGGAGAAGA
PRIMER_LEFT_25=754,20
PRIMER_RIGHT_25=1678,20
PRIMER_LEFT_25_TM=59.143
PRIMER_RIGHT_25_TM=59.665
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=10.0000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=925
PRIMER_PAIR_26_PENALTY=1.220320
PRIMER_LEFT_26_PENALTY=0.232299
PRIMER_RIGHT_26_PENALTY=0.988020
PRIMER_LEFT_26_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_26_SEQUENCE=GAAACGGGTGGGAGAAGAAG
PRIMER_LEFT_26=764,20
PRIMER_RIGHT_26=1676,20
PRIMER_LEFT_26_TM=60.232
PRIMER_RIGHT_26_TM=60.988
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=913
PRIMER_PAIR_27_PENALTY=1.220320
PRIMER_LEFT_27_PENALTY=0.232299
PRIMER_RIGHT_27_PENALTY=0.988020
PRIMER_LEFT_27_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_27_SEQUENCE=AGAAACGGGTGGGAGAAGAA
PRIMER_LEFT_27=764,20
PRIMER_RIGHT_27=1677,20
PRIMER_LEFT_27_TM=60.232
PRIMER_RIGHT_27_TM=60.988
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=914
PRIMER_PAIR_28_PENALTY=1.233844
PRIMER_LEFT_28_PENALTY=0.857208
PRIMER_RIGHT_28_PENALTY=0.376636
PRIMER_LEFT_28_SEQUENCE=GTCGTGTTCATGAGTTTGCC
PRIMER_RIGHT_28_SEQUENCE=TAATCATGGTGGTGGTGGAA
PRIMER_LEFT_28=754,20
PRIMER_RIGHT_28=1651,20
PRIMER_LEFT_28_TM=59.143
PRIMER_RIGHT_28_TM=59.623
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=10.0000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=898
PRIMER_PAIR_29_PENALTY=1.259564
PRIMER_LEFT_29_PENALTY=1.171447
PRIMER_RIGHT_29_PENALTY=0.088117
PRIMER_LEFT_29_SEQUENCE=GTCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_29_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_29=754,21
PRIMER_RIGHT_29=1654,20
PRIMER_LEFT_29_TM=60.171
PRIMER_RIGHT_29_TM=60.088
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=901
PRIMER_PAIR_30_PENALTY=1.282845
PRIMER_LEFT_30_PENALTY=0.707326
PRIMER_RIGHT_30_PENALTY=0.575519
PRIMER_LEFT_30_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_30_SEQUENCE=CTGAATTAGAAACGGGTGGG
PRIMER_LEFT_30=756,20
PRIMER_RIGHT_30=1684,20
PRIMER_LEFT_30_TM=59.293
PRIMER_RIGHT_30_TM=59.424
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=929
PRIMER_PAIR_31_PENALTY=1.282845
PRIMER_LEFT_31_PENALTY=0.707326
PRIMER_RIGHT_31_PENALTY=0.575519
PRIMER_LEFT_31_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_31_SEQUENCE=CCTGAATTAGAAACGGGTGG
PRIMER_LEFT_31=756,20
PRIMER_RIGHT_31=1685,20
PRIMER_LEFT_31_TM=59.293
PRIMER_RIGHT_31_TM=59.424
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=930
PRIMER_PAIR_32_PENALTY=1.282845
PRIMER_LEFT_32_PENALTY=0.707326
PRIMER_RIGHT_32_PENALTY=0.575519
PRIMER_LEFT_32_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_32_SEQUENCE=CTGAATTAGAAACGGGTGGG
PRIMER_LEFT_32=755,20
PRIMER_RIGHT_32=1684,20
PRIMER_LEFT_32_TM=59.293
PRIMER_RIGHT_32_TM=59.424
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=930
PRIMER_PAIR_33_PENALTY=1.282845
PRIMER_LEFT_33_PENALTY=0.707326
PRIMER_RIGHT_33_PENALTY=0.575519
PRIMER_LEFT_33_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_33_SEQUENCE=CCTGAATTAGAAACGGGTGG
PRIMER_LEFT_33=755,20
PRIMER_RIGHT_33=1685,20
PRIMER_LEFT_33_TM=59.293
PRIMER_RIGHT_33_TM=59.424
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=1.288265
PRIMER_LEFT_34_PENALTY=0.857208
PRIMER_RIGHT_34_PENALTY=0.431057
PRIMER_LEFT_34_SEQUENCE=GTCGTGTTCATGAGTTTGCC
PRIMER_RIGHT_34_SEQUENCE=GCCTGAATTAGAAACGGGTG
PRIMER_LEFT_34=754,20
PRIMER_RIGHT_34=1686,20
PRIMER_LEFT_34_TM=59.143
PRIMER_RIGHT_34_TM=59.569
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.0000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=933
PRIMER_PAIR_35_PENALTY=1.307693
PRIMER_LEFT_35_PENALTY=0.232299
PRIMER_RIGHT_35_PENALTY=1.075394
PRIMER_LEFT_35_SEQUENCE=TGAGTTTGCCTCACCTTTCC
PRIMER_RIGHT_35_SEQUENCE=GATTGGTAATCATGGTGGTGG
PRIMER_LEFT_35=764,20
PRIMER_RIGHT_35=1657,21
PRIMER_LEFT_35_TM=60.232
PRIMER_RIGHT_35_TM=59.925
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=894
PRIMER_PAIR_36_PENALTY=1.328113
PRIMER_LEFT_36_PENALTY=1.239996
PRIMER_RIGHT_36_PENALTY=0.088117
PRIMER_LEFT_36_SEQUENCE=GAGTTTGCCTCACCTTTCCTC
PRIMER_RIGHT_36_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_36=765,21
PRIMER_RIGHT_36=1654,20
PRIMER_LEFT_36_TM=60.240
PRIMER_RIGHT_36_TM=60.088
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=890
PRIMER_PAIR_37_PENALTY=1.345029
PRIMER_LEFT_37_PENALTY=1.256912
PRIMER_RIGHT_37_PENALTY=0.088117
PRIMER_LEFT_37_SEQUENCE=GTTTGCCTCACCTTTCCTCTT
PRIMER_RIGHT_37_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_37=767,21
PRIMER_RIGHT_37=1654,20
PRIMER_LEFT_37_TM=59.743
PRIMER_RIGHT_37_TM=60.088
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=888
PRIMER_PAIR_38_PENALTY=1.351889
PRIMER_LEFT_38_PENALTY=0.707326
PRIMER_RIGHT_38_PENALTY=0.644562
PRIMER_LEFT_38_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_38_SEQUENCE=GGTGGTGGAATGTGATTCGT
PRIMER_LEFT_38=756,20
PRIMER_RIGHT_38=1641,20
PRIMER_LEFT_38_TM=59.293
PRIMER_RIGHT_38_TM=60.645
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=886
PRIMER_PAIR_39_PENALTY=1.351889
PRIMER_LEFT_39_PENALTY=0.707326
PRIMER_RIGHT_39_PENALTY=0.644562
PRIMER_LEFT_39_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_39_SEQUENCE=GGTGGTGGAATGTGATTCGT
PRIMER_LEFT_39=755,20
PRIMER_RIGHT_39=1641,20
PRIMER_LEFT_39_TM=59.293
PRIMER_RIGHT_39_TM=60.645
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=8.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=887
PRIMER_PAIR_40_PENALTY=1.359451
PRIMER_LEFT_40_PENALTY=0.707326
PRIMER_RIGHT_40_PENALTY=0.652125
PRIMER_LEFT_40_SEQUENCE=CGTGTTCATGAGTTTGCCTC
PRIMER_RIGHT_40_SEQUENCE=GGTGGAATGTGATTCGTCAA
PRIMER_LEFT_40=756,20
PRIMER_RIGHT_40=1638,20
PRIMER_LEFT_40_TM=59.293
PRIMER_RIGHT_40_TM=59.348
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=883
PRIMER_PAIR_41_PENALTY=1.359451
PRIMER_LEFT_41_PENALTY=0.707326
PRIMER_RIGHT_41_PENALTY=0.652125
PRIMER_LEFT_41_SEQUENCE=TCGTGTTCATGAGTTTGCCT
PRIMER_RIGHT_41_SEQUENCE=GGTGGAATGTGATTCGTCAA
PRIMER_LEFT_41=755,20
PRIMER_RIGHT_41=1638,20
PRIMER_LEFT_41_TM=59.293
PRIMER_RIGHT_41_TM=59.348
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=884
PRIMER_PAIR_42_PENALTY=1.362381
PRIMER_LEFT_42_PENALTY=1.274264
PRIMER_RIGHT_42_PENALTY=0.088117
PRIMER_LEFT_42_SEQUENCE=CATGAGTTTGCCTCACCTTTC
PRIMER_RIGHT_42_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_42=762,21
PRIMER_RIGHT_42=1654,20
PRIMER_LEFT_42_TM=59.726
PRIMER_RIGHT_42_TM=60.088
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=893
PRIMER_PAIR_43_PENALTY=1.362381
PRIMER_LEFT_43_PENALTY=1.274264
PRIMER_RIGHT_43_PENALTY=0.088117
PRIMER_LEFT_43_SEQUENCE=TCATGAGTTTGCCTCACCTTT
PRIMER_RIGHT_43_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_43=761,21
PRIMER_RIGHT_43=1654,20
PRIMER_LEFT_43_TM=59.726
PRIMER_RIGHT_43_TM=60.088
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=894
PRIMER_PAIR_44_PENALTY=1.362381
PRIMER_LEFT_44_PENALTY=1.274264
PRIMER_RIGHT_44_PENALTY=0.088117
PRIMER_LEFT_44_SEQUENCE=TTCATGAGTTTGCCTCACCTT
PRIMER_RIGHT_44_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_44=760,21
PRIMER_RIGHT_44=1654,20
PRIMER_LEFT_44_TM=59.726
PRIMER_RIGHT_44_TM=60.088
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=895
PRIMER_PAIR_45_PENALTY=1.403023
PRIMER_LEFT_45_PENALTY=1.314907
PRIMER_RIGHT_45_PENALTY=0.088117
PRIMER_LEFT_45_SEQUENCE=TGTTTTCATGCATCCCTCTCT
PRIMER_RIGHT_45_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_45=894,21
PRIMER_RIGHT_45=1654,20
PRIMER_LEFT_45_TM=59.685
PRIMER_RIGHT_45_TM=60.088
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=761
PRIMER_PAIR_46_PENALTY=1.432727
PRIMER_LEFT_46_PENALTY=0.857208
PRIMER_RIGHT_46_PENALTY=0.575519
PRIMER_LEFT_46_SEQUENCE=GTCGTGTTCATGAGTTTGCC
PRIMER_RIGHT_46_SEQUENCE=CTGAATTAGAAACGGGTGGG
PRIMER_LEFT_46=754,20
PRIMER_RIGHT_46=1684,20
PRIMER_LEFT_46_TM=59.143
PRIMER_RIGHT_46_TM=59.424
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=10.0000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=931
PRIMER_PAIR_47_PENALTY=1.432727
PRIMER_LEFT_47_PENALTY=0.857208
PRIMER_RIGHT_47_PENALTY=0.575519
PRIMER_LEFT_47_SEQUENCE=GTCGTGTTCATGAGTTTGCC
PRIMER_RIGHT_47_SEQUENCE=CCTGAATTAGAAACGGGTGG
PRIMER_LEFT_47=754,20
PRIMER_RIGHT_47=1685,20
PRIMER_LEFT_47_TM=59.143
PRIMER_RIGHT_47_TM=59.424
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=10.0000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=932
PRIMER_PAIR_48_PENALTY=1.442779
PRIMER_LEFT_48_PENALTY=1.066143
PRIMER_RIGHT_48_PENALTY=0.376636
PRIMER_LEFT_48_SEQUENCE=TTGTTTTCATGCATCCCTCTC
PRIMER_RIGHT_48_SEQUENCE=TAATCATGGTGGTGGTGGAA
PRIMER_LEFT_48=893,21
PRIMER_RIGHT_48=1651,20
PRIMER_LEFT_48_TM=60.066
PRIMER_RIGHT_48_TM=59.623
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=759
PRIMER_PAIR_49_PENALTY=1.443523
PRIMER_LEFT_49_PENALTY=1.355407
PRIMER_RIGHT_49_PENALTY=0.088117
PRIMER_LEFT_49_SEQUENCE=TGTTTTCATGCATCCCTCTC
PRIMER_RIGHT_49_SEQUENCE=TGGTAATCATGGTGGTGGTG
PRIMER_LEFT_49=894,20
PRIMER_RIGHT_49=1654,20
PRIMER_LEFT_49_TM=58.645
PRIMER_RIGHT_49_TM=60.088
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=761
=
SEQUENCE_ID=MAGIC.433_4_1626296_1626434_po-0_edi-0_13675_895_TRUE
SEQUENCE_TARGET=950,238
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4335995_4336072_edi-0_sf-2_8935_65967_TRUE
SEQUENCE_TARGET=950,177
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_24018791_24019077_kn-0_wil-2_613521_32730_TRUE
SEQUENCE_TARGET=950,386
SEQUENCE_TEMPLATE=AGTTTCCNCAGNNNGAAACAAATGAAAACGACGGTATNTTATGCATATAAGGTCNANACTATTGTTTTATNTTTAGAAAGGTAGTCAAATTATTTNTGTGTTAACTCAAGTATCATAAACTAAAACATGTTATGTCAGTGGTTGCTGTCCAAAACATCAAAACCACCTATGNAATNAGTAGAAANAGCTGGCAAGGAGATCCATGTGTCCCTCGGCAGTTTATGTGGGAGGGATTAAACTGCAGTGANACTGATATGNCNACACNACCAANAATCACTTCTTTGTAAGNCNTANGANNNNNNNNANTACAATAAAAACTGNNNNNNNNNNNNNNNATTATAGTTANAATAAAAAGATATTNGNTCACAATANTTTTTTTGTGNCGAAANTGANANAAANTTTANAATNTCACAATAAAATCGTCACAAATNTATGTATGTCTNAAAAANNTCACAAACGAATGTNACGATTAACAACAGNAACAATTTGTCACTGATTGTGACGANGTTATTATAGTCGCAAAAACGGANACAATTCGTGACGAACGATACTNTCACAACTATNCCACAACTAGTGACAATAAAATNGTAACATACTTGTCACAGATTATTACCATATTTAAGTCACTGGCCCGTTACATTTTACGACCAAAATACAATNACAAATCCGTNACAATTAGTNACTCGATTATAGTCACACAAACGTCACAAATTGATTTTATGTTGTGACANTTTTTCTTACTAAACTCGTATCAATTTGTTACAAAACTAGTTTCATNAACAACACATGGACGTTTGGTCACAAACGTATGATTACCTTNTTNTGCTTTTACTAATTGCAGTNATTCTCTTTATAAAATTATAACGANCATATTTTATAGAAATNATTAAACAACATCCGATAAACAANTTGACTAATAGAAACCATAATTTATAGAAATCATAAAACAATATCCAAATAAACAAAATACAATACTTAGGNTAATGCAAATAAAACAAACNATAACATCCAAACAAACAANNAACATCCAAATAAAGTCAAACTAGAAACNCAACTTAAAGNAAATACAAACTAAACAATACTTAGGCTAAAAGGAACCATACATAAGATAGATTAAGTGGACGTTCTTGGTGGTGGNTTTGGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_26196564_26196581_sf-2_ler-0_310621_177981_TRUE
SEQUENCE_TARGET=950,117
SEQUENCE_TEMPLATE=ATCTTTATTCTTCAATTGGGGNAATTTGATTATTGAAACGATGATTGGGGATTGTTTTAGGGTTTGGGCGTTGTTGAGTTGACAAATCAACGATGAAGNAAGAAACAAAACAAAGGACGATNCTTGCGTTTTGGTCTGAGAGACAGACGTGGCTGAACTGTACTCATTGAAAANAAAANANAANNAAATATCTAATTATCCAGTGTGNTTAGATCTACAGTGANTTATCTACGTGGCGGAATCTCGATCGTATATACTTTTGATGTGATTTNTAACGGTTGATTCGNTAAGAAGAATCTCGTACTTACCGTGTTTGATTNTTATTACTGTTAGNGATGGCGTGATTGGTTGTTTTGTTCCTCGTTTTAGTTTCATGTCGTCTTTGAAACNACGCCGTATAAGTTTAAACATATGTGACGAATGTATAAAATTCATCTTTACTANCTCTCATAATTAATGAATTAATNAAAAAAAAANTCNGTATCCGAAGTTNTACTACCGATAATACTAATTGAATGAAGGCAANCTCTTNTATTTTATGTTTCTATAAAATAGAAGAGATTNTTAACCATGAGAGATAAATGGTTAANAATGATAGATAACTAGATATTATGNGAAANTTNTAGATAAAACNTTTTCAAGAAACTCATTTTCGATATAGNANATAAAANCATTGTCNATAAACATTNGAAAATAGATTGGGAGATTATGNAAAATCNNNNNTTNTTTTTGTCTCTTATAAAATANNNTNATTTGCNNATATANCANTTTTTNACTAAATAATTAACTAACTAACACNATAAATTTGTAAATGTAATAAATAACNTTNANNGGGTGTGAAAAGACATCANTNTCCTGNTGTTACGTNTGTCAGCGAGGANGGAAAATGTTTAGTGTGAGNTGACGTGNCTNTATTATTNGTGTTGAGGTNAGAAAAGAAATCAGGTGGTAGGAGAGAAGTTCCTTTTTCTCTTTCNTTTTTCTTTACCCCTTCCCAATTNTAATTCATATTATCTANTCTTNAAAAAATTAATTNAAGTTCATGATATATCAATNAAAAGTTGTATAGTTTTCTANATATATCATNACTTNAATTTTTTAAAACANACACAAAATAATTTAGATGTNAATAGTGTTGTCAATGAATAANATAACNTTCTATATANATANANNATATTTTTTNATGNAAAGATATTAAACCNTAAGGGATAATATATAGNGAACCCAAGGGTTTAAATCTAAACCAATAATTAAATCACTTAACATCCTTAATCAAAACAAATAATTCATTTTAAAACGTATATAAAACAAAGTATACGTAAATAGTATATTTACAAATAGAATAGTATCTCTCAAATTNCAACAACAAAACTCNATTTCATCACCAAGACACCAAGCGCAACAACGAAAGTCGATTCCCTGAATCCAAATTGTGTTCCTCCCTCTGTAATTNGAAACCCCCAAATCAAATCTNACCCAAAAGTAATTAAGAGACTCTGATTTGAAAACAAAAACAAGTAACNATGTANGTGTAAGGACTTTACNNGGGAGAAAGAGAGANGGNGAGGGAAAANNNNNNNNNNAGAAAGAGAGAGGGGGNNNNNNNNNNAGAGNNNNNNNNNNNNNNNNNNNNNNNAGANGTACACNTAAAGANANATGANAGAAANAGATANAGNGGTAAANTTGGAATTAATATACAAAATATAGAGNATATNNTTNTTTTTNTTTTNATGTTAGTTNCTTAATTATAAANTTTTTGTTGGTTAGTTTTGNCAATTCNCCTATAAAATAGAATTAATAGANAACCNGNTGNNAACATNANNNNNANAANAAAAANAACTGGTCCANGACATAGCATTGTGTCTTAGTAATCTACTGGTTCNTATTCTACTCATGTAGAAGTAAATTAATAAGNAAACAAAACAAAAAAGAGTTTAGATAATGGTAGAGAATTTGGTCGCGGAAACAACTTCAAATTCGGCCTCAATCATCAACGTGTCTTCTTTGTCCAAGTAAG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_15380498_15381063_oy-0_ws-0_69078_4123_TRUE
SEQUENCE_TARGET=950,665
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17167752_17168088_edi-0_col-0_12670_16637_TRUE
SEQUENCE_TARGET=950,436
SEQUENCE_TEMPLATE=TGAATTGTCAAATAACAATCTTACTACAGATGCTTCTTTAGATTATCTAGATCATGCAAATGTCTGCTATATGAAAGCGTACCCGGATGAGACGGTTTTTGTGATACACATTGAAACCGCAGATTGCAAGCTTAGGTGCCTCCTTTACAAATCCAATTTTGATTTCNGTGGAAGCNTGTAACAAGAAAGCCTAGTGTTTTTTTTTTCTCATTCCCTAGATTAATGTTTGCATATGAAAACACAAACAGTTATGCAACGGCAAATAGCAAAACAATTAGCCAAAGCTCCACATAACAAAACTAGAGAGTAGAAAAGAAAAAAAGGAATCACTTGTTCCGTTGTAGCTGTATGGGGCTTGTACTTAATAATCTCGGGAAATCTGAATCCATCAGCAATGTTGAACTGCTCCACAGGGATTCCCCGAATTATAATTTTGAAGTTTTTGAACTTTTTGAGATATAACATTGAAGCATAAGCCTAGGGATGAAGAAATAACAAAATGAATCTTCAGACATCCTTGGAACAAAACATAATTAGAAAAAGGAAGAAAATATTAAGGAGATTACCCTCAGAGAATAACGTAAGTGGTATGAAATATGAGATCTCAGTTCCAATATTTTATGGTGCAACCGTTTTCCGTCATTCACACTCTCATCTCGGAGCCGGATATCCTATACCAATATCAAAACATATGACCAGACCTAAAACAAAAACACCTCTGGCAAGATATCGCGTCGAGTATATANCAATTGAGGAAAAGTGTGTGTTACCTCCTCGTCGTCGTCAAAACTCAGCTCATAGATTCCTTCATCATTAAGCCACAAGTTGTATATAATTACTTTTGTTCCATGAGTTCCAACGTNCTCGAACTGGTGGGTGGAAGATGGAAATAAAAAAATTAACAAAACAGACTGCAATCACATGGAATAAAAATCTTCAGACTGCTTTAGGGGAACTGAAGCAAATTGGGACACAAGTCAAGGCAGAACAGTAATGCAATNAAATATTCATAAGCTGACTTTCAAAAGAAACAACCTGCTGCAAAAGTTCGTCCTCTGTAGAAAACGGAGACCATTTGAGTAGAATTTCAAGGTTGGCAGCCCAATCTTCTGGAGATCCATAGATAATTGGTTGTGGTCGNTCCTTGGATATATCAATATCAATCTGCATTACATATCGGATCTATCACTTGAGAACAAAGAAGAGTTGCACATTTTCGAGTTTCCAAGAATTATTCATTTCAGGAGTGAAACAAAATCTACAAATGCTGAAGATTTTACCATAGGAACAGTGACATCATCCTGACCAGTTTTTCTAAGGAATGTGTATGACAGAANGCCAACGCTCTGAGTAGATGTGCTAAAAGAGAGAAGAATAAAAAGGTTAANNNAANAGCAAACGACATATTCAGAGTGAATATGACCAGATATATCAATACCCTCCACGAGTGGAGCGACTAAAAACGATAGCATCAGCTCCAAGTCTCATGGTACTTGTCTTGAAACCATTTCCATCTAAACACAAAAGGGAAGCTCTGGTTAGAGACCACCTGTCGGAAACCAAGACAGCAAGACCACCCGACTAGAAACGAGCTCAAGATCATATTAACTAAGNAAAACAATTACACTGTCCAATCGTTGTATTAGACTTCTTTGATGAGTAGCCTAAGCTCATGCATTTTCTGAGCCCAGCGGGATCCATCCCACCACCATCATCTTCAAGTTCAAAAAGAAAGGAGAGAAACCATATCAATAACAACATCCCAAGCCTTGGTTAGGCAAAAGCACCACAGTCATTCAAAGGAAATGTACCTTGGAAAACTAAAGCTGGGGAATTATCTTTGACAATATTGATCTTATCAATCTTAACAAACGTAGCACCATTTTGTATCTGAGATATAAGTAATTCAAATTAACCAAACACGGTACNGATGCTTTAAAAAANCAGATCACTCGCAACAATCAAAAACGAAAAAGATAATAAAAAATGAACTTANGAAAGCATTTCAATCCTCACACATACCTCGTCAACAGCATTGTCAAGTAGCTCAGCGATTGCTGCAATACAATACAAAGAAGAAGAAATTGACAAAGATGATCATCACAAAATCACAAATTAATCAAATTGATCGCACCAGTAGAGACAATTCATAGGCTCATAGCTTTACCTCCAAAAGCCCATTTGTGTGAGGTAGCATTCGAGTGAAGAAACCTGGGATGAANTCGAGCATGCTCAAGCATACCTATCAAATTCCATCTCATTAGATACCATAATCGTGATGCATGCGTGTGTATGAGGAAGAGAGAGATGACCTGGAGCAGTAGGAGTCACAACGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.135740
PRIMER_LEFT_0_PENALTY=0.024663
PRIMER_RIGHT_0_PENALTY=0.111077
PRIMER_LEFT_0_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_0_SEQUENCE=TCGTTTCTAGTCGGGTGGTC
PRIMER_LEFT_0=773,20
PRIMER_RIGHT_0=1590,20
PRIMER_LEFT_0_TM=60.025
PRIMER_RIGHT_0_TM=60.111
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=818
PRIMER_PAIR_1_PENALTY=0.157232
PRIMER_LEFT_1_PENALTY=0.024663
PRIMER_RIGHT_1_PENALTY=0.132570
PRIMER_LEFT_1_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_1_SEQUENCE=TCTAGTCGGGTGGTCTTGCT
PRIMER_LEFT_1=773,20
PRIMER_RIGHT_1=1585,20
PRIMER_LEFT_1_TM=60.025
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=813
PRIMER_PAIR_2_PENALTY=0.183385
PRIMER_LEFT_2_PENALTY=0.024663
PRIMER_RIGHT_2_PENALTY=0.158723
PRIMER_LEFT_2_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_2_SEQUENCE=GGTGGTCTTGCTGTCTTGGT
PRIMER_LEFT_2=773,20
PRIMER_RIGHT_2=1577,20
PRIMER_LEFT_2_TM=60.025
PRIMER_RIGHT_2_TM=60.159
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=805
PRIMER_PAIR_3_PENALTY=0.474066
PRIMER_LEFT_3_PENALTY=0.362989
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_LEFT_3_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_3_SEQUENCE=TCGTTTCTAGTCGGGTGGTC
PRIMER_LEFT_3=866,20
PRIMER_RIGHT_3=1590,20
PRIMER_LEFT_3_TM=60.363
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=725
PRIMER_PAIR_4_PENALTY=0.495559
PRIMER_LEFT_4_PENALTY=0.362989
PRIMER_RIGHT_4_PENALTY=0.132570
PRIMER_LEFT_4_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_4_SEQUENCE=TCTAGTCGGGTGGTCTTGCT
PRIMER_LEFT_4=866,20
PRIMER_RIGHT_4=1585,20
PRIMER_LEFT_4_TM=60.363
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=720
PRIMER_PAIR_5_PENALTY=0.521712
PRIMER_LEFT_5_PENALTY=0.362989
PRIMER_RIGHT_5_PENALTY=0.158723
PRIMER_LEFT_5_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_5_SEQUENCE=GGTGGTCTTGCTGTCTTGGT
PRIMER_LEFT_5=866,20
PRIMER_RIGHT_5=1577,20
PRIMER_LEFT_5_TM=60.363
PRIMER_RIGHT_5_TM=60.159
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=712
PRIMER_PAIR_6_PENALTY=0.528359
PRIMER_LEFT_6_PENALTY=0.024663
PRIMER_RIGHT_6_PENALTY=0.503696
PRIMER_LEFT_6_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_6_SEQUENCE=AGCTCGTTTCTAGTCGGGTG
PRIMER_LEFT_6=773,20
PRIMER_RIGHT_6=1593,20
PRIMER_LEFT_6_TM=60.025
PRIMER_RIGHT_6_TM=59.496
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=821
PRIMER_PAIR_7_PENALTY=0.553523
PRIMER_LEFT_7_PENALTY=0.024663
PRIMER_RIGHT_7_PENALTY=0.528861
PRIMER_LEFT_7_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_7_SEQUENCE=TGAGCTCGTTTCTAGTCGGG
PRIMER_LEFT_7=773,20
PRIMER_RIGHT_7=1595,20
PRIMER_LEFT_7_TM=60.025
PRIMER_RIGHT_7_TM=60.529
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=11.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=823
PRIMER_PAIR_8_PENALTY=0.624927
PRIMER_LEFT_8_PENALTY=0.024663
PRIMER_RIGHT_8_PENALTY=0.600264
PRIMER_LEFT_8_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_8_SEQUENCE=AAGATGATGGTGGTGGGATG
PRIMER_LEFT_8=773,20
PRIMER_RIGHT_8=1717,20
PRIMER_LEFT_8_TM=60.025
PRIMER_RIGHT_8_TM=60.600
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=945
PRIMER_PAIR_9_PENALTY=0.683599
PRIMER_LEFT_9_PENALTY=0.362989
PRIMER_RIGHT_9_PENALTY=0.320610
PRIMER_LEFT_9_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_9_SEQUENCE=TCCCCAGCTTTAGTTTTCCA
PRIMER_LEFT_9=866,20
PRIMER_RIGHT_9=1832,20
PRIMER_LEFT_9_TM=60.363
PRIMER_RIGHT_9_TM=59.679
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=967
PRIMER_PAIR_10_PENALTY=0.696260
PRIMER_LEFT_10_PENALTY=0.024663
PRIMER_RIGHT_10_PENALTY=0.671597
PRIMER_LEFT_10_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_10_SEQUENCE=CCAGAGCTTCCCTTTTGTGT
PRIMER_LEFT_10=773,20
PRIMER_RIGHT_10=1537,20
PRIMER_LEFT_10_TM=60.025
PRIMER_RIGHT_10_TM=59.328
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=765
PRIMER_PAIR_11_PENALTY=0.696260
PRIMER_LEFT_11_PENALTY=0.024663
PRIMER_RIGHT_11_PENALTY=0.671597
PRIMER_LEFT_11_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_11_SEQUENCE=ACCAGAGCTTCCCTTTTGTG
PRIMER_LEFT_11=773,20
PRIMER_RIGHT_11=1538,20
PRIMER_LEFT_11_TM=60.025
PRIMER_RIGHT_11_TM=59.328
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=766
PRIMER_PAIR_12_PENALTY=0.704244
PRIMER_LEFT_12_PENALTY=0.024663
PRIMER_RIGHT_12_PENALTY=0.679581
PRIMER_LEFT_12_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_12_SEQUENCE=CTGTCTTGGTTTCCGACAGG
PRIMER_LEFT_12=773,20
PRIMER_RIGHT_12=1567,20
PRIMER_LEFT_12_TM=60.025
PRIMER_RIGHT_12_TM=60.680
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=795
PRIMER_PAIR_13_PENALTY=0.735847
PRIMER_LEFT_13_PENALTY=0.024663
PRIMER_RIGHT_13_PENALTY=0.711184
PRIMER_LEFT_13_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_13_SEQUENCE=TAGTCGGGTGGTCTTGCTGT
PRIMER_LEFT_13=773,20
PRIMER_RIGHT_13=1583,20
PRIMER_LEFT_13_TM=60.025
PRIMER_RIGHT_13_TM=60.711
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=811
PRIMER_PAIR_14_PENALTY=0.816899
PRIMER_LEFT_14_PENALTY=0.024663
PRIMER_RIGHT_14_PENALTY=0.792236
PRIMER_LEFT_14_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_14_SEQUENCE=CGTTTCTAGTCGGGTGGTCT
PRIMER_LEFT_14=773,20
PRIMER_RIGHT_14=1589,20
PRIMER_LEFT_14_TM=60.025
PRIMER_RIGHT_14_TM=59.208
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=817
PRIMER_PAIR_15_PENALTY=0.816899
PRIMER_LEFT_15_PENALTY=0.024663
PRIMER_RIGHT_15_PENALTY=0.792236
PRIMER_LEFT_15_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_15_SEQUENCE=CTCGTTTCTAGTCGGGTGGT
PRIMER_LEFT_15=773,20
PRIMER_RIGHT_15=1591,20
PRIMER_LEFT_15_TM=60.025
PRIMER_RIGHT_15_TM=59.208
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=819
PRIMER_PAIR_16_PENALTY=0.866685
PRIMER_LEFT_16_PENALTY=0.362989
PRIMER_RIGHT_16_PENALTY=0.503696
PRIMER_LEFT_16_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_16_SEQUENCE=AGCTCGTTTCTAGTCGGGTG
PRIMER_LEFT_16=866,20
PRIMER_RIGHT_16=1593,20
PRIMER_LEFT_16_TM=60.363
PRIMER_RIGHT_16_TM=59.496
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=728
PRIMER_PAIR_17_PENALTY=0.868002
PRIMER_LEFT_17_PENALTY=0.024663
PRIMER_RIGHT_17_PENALTY=0.843339
PRIMER_LEFT_17_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_17_SEQUENCE=CTAGTCGGGTGGTCTTGCTG
PRIMER_LEFT_17=773,20
PRIMER_RIGHT_17=1584,20
PRIMER_LEFT_17_TM=60.025
PRIMER_RIGHT_17_TM=60.843
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=60.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=812
PRIMER_PAIR_18_PENALTY=0.871556
PRIMER_LEFT_18_PENALTY=0.024663
PRIMER_RIGHT_18_PENALTY=0.846894
PRIMER_LEFT_18_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_18_SEQUENCE=GTTTCCGACAGGTGGTCTCT
PRIMER_LEFT_18=773,20
PRIMER_RIGHT_18=1559,20
PRIMER_LEFT_18_TM=60.025
PRIMER_RIGHT_18_TM=59.153
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=787
PRIMER_PAIR_19_PENALTY=0.891850
PRIMER_LEFT_19_PENALTY=0.362989
PRIMER_RIGHT_19_PENALTY=0.528861
PRIMER_LEFT_19_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_19_SEQUENCE=TGAGCTCGTTTCTAGTCGGG
PRIMER_LEFT_19=866,20
PRIMER_RIGHT_19=1595,20
PRIMER_LEFT_19_TM=60.363
PRIMER_RIGHT_19_TM=60.529
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=11.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=730
PRIMER_PAIR_20_PENALTY=0.944070
PRIMER_LEFT_20_PENALTY=0.832993
PRIMER_RIGHT_20_PENALTY=0.111077
PRIMER_LEFT_20_SEQUENCE=CCTCGTCGTCGTCAAAACTC
PRIMER_RIGHT_20_SEQUENCE=TCGTTTCTAGTCGGGTGGTC
PRIMER_LEFT_20=772,20
PRIMER_RIGHT_20=1590,20
PRIMER_LEFT_20_TM=60.833
PRIMER_RIGHT_20_TM=60.111
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=819
PRIMER_PAIR_21_PENALTY=0.944070
PRIMER_LEFT_21_PENALTY=0.832993
PRIMER_RIGHT_21_PENALTY=0.111077
PRIMER_LEFT_21_SEQUENCE=TCCTCGTCGTCGTCAAAACT
PRIMER_RIGHT_21_SEQUENCE=TCGTTTCTAGTCGGGTGGTC
PRIMER_LEFT_21=771,20
PRIMER_RIGHT_21=1590,20
PRIMER_LEFT_21_TM=60.833
PRIMER_RIGHT_21_TM=60.111
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=820
PRIMER_PAIR_22_PENALTY=0.944070
PRIMER_LEFT_22_PENALTY=0.832993
PRIMER_RIGHT_22_PENALTY=0.111077
PRIMER_LEFT_22_SEQUENCE=CTCCTCGTCGTCGTCAAAAC
PRIMER_RIGHT_22_SEQUENCE=TCGTTTCTAGTCGGGTGGTC
PRIMER_LEFT_22=770,20
PRIMER_RIGHT_22=1590,20
PRIMER_LEFT_22_TM=60.833
PRIMER_RIGHT_22_TM=60.111
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=821
PRIMER_PAIR_23_PENALTY=0.963253
PRIMER_LEFT_23_PENALTY=0.362989
PRIMER_RIGHT_23_PENALTY=0.600264
PRIMER_LEFT_23_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_23_SEQUENCE=AAGATGATGGTGGTGGGATG
PRIMER_LEFT_23=866,20
PRIMER_RIGHT_23=1717,20
PRIMER_LEFT_23_TM=60.363
PRIMER_RIGHT_23_TM=60.600
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=8.1000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=852
PRIMER_PAIR_24_PENALTY=0.965563
PRIMER_LEFT_24_PENALTY=0.832993
PRIMER_RIGHT_24_PENALTY=0.132570
PRIMER_LEFT_24_SEQUENCE=CCTCGTCGTCGTCAAAACTC
PRIMER_RIGHT_24_SEQUENCE=TCTAGTCGGGTGGTCTTGCT
PRIMER_LEFT_24=772,20
PRIMER_RIGHT_24=1585,20
PRIMER_LEFT_24_TM=60.833
PRIMER_RIGHT_24_TM=59.867
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=814
PRIMER_PAIR_25_PENALTY=0.965563
PRIMER_LEFT_25_PENALTY=0.832993
PRIMER_RIGHT_25_PENALTY=0.132570
PRIMER_LEFT_25_SEQUENCE=TCCTCGTCGTCGTCAAAACT
PRIMER_RIGHT_25_SEQUENCE=TCTAGTCGGGTGGTCTTGCT
PRIMER_LEFT_25=771,20
PRIMER_RIGHT_25=1585,20
PRIMER_LEFT_25_TM=60.833
PRIMER_RIGHT_25_TM=59.867
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=815
PRIMER_PAIR_26_PENALTY=0.965563
PRIMER_LEFT_26_PENALTY=0.832993
PRIMER_RIGHT_26_PENALTY=0.132570
PRIMER_LEFT_26_SEQUENCE=CTCCTCGTCGTCGTCAAAAC
PRIMER_RIGHT_26_SEQUENCE=TCTAGTCGGGTGGTCTTGCT
PRIMER_LEFT_26=770,20
PRIMER_RIGHT_26=1585,20
PRIMER_LEFT_26_TM=60.833
PRIMER_RIGHT_26_TM=59.867
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=816
PRIMER_PAIR_27_PENALTY=0.991716
PRIMER_LEFT_27_PENALTY=0.832993
PRIMER_RIGHT_27_PENALTY=0.158723
PRIMER_LEFT_27_SEQUENCE=CCTCGTCGTCGTCAAAACTC
PRIMER_RIGHT_27_SEQUENCE=GGTGGTCTTGCTGTCTTGGT
PRIMER_LEFT_27=772,20
PRIMER_RIGHT_27=1577,20
PRIMER_LEFT_27_TM=60.833
PRIMER_RIGHT_27_TM=60.159
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=806
PRIMER_PAIR_28_PENALTY=0.991716
PRIMER_LEFT_28_PENALTY=0.832993
PRIMER_RIGHT_28_PENALTY=0.158723
PRIMER_LEFT_28_SEQUENCE=TCCTCGTCGTCGTCAAAACT
PRIMER_RIGHT_28_SEQUENCE=GGTGGTCTTGCTGTCTTGGT
PRIMER_LEFT_28=771,20
PRIMER_RIGHT_28=1577,20
PRIMER_LEFT_28_TM=60.833
PRIMER_RIGHT_28_TM=60.159
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=807
PRIMER_PAIR_29_PENALTY=0.991716
PRIMER_LEFT_29_PENALTY=0.832993
PRIMER_RIGHT_29_PENALTY=0.158723
PRIMER_LEFT_29_SEQUENCE=CTCCTCGTCGTCGTCAAAAC
PRIMER_RIGHT_29_SEQUENCE=GGTGGTCTTGCTGTCTTGGT
PRIMER_LEFT_29=770,20
PRIMER_RIGHT_29=1577,20
PRIMER_LEFT_29_TM=60.833
PRIMER_RIGHT_29_TM=60.159
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=808
PRIMER_PAIR_30_PENALTY=1.034586
PRIMER_LEFT_30_PENALTY=0.362989
PRIMER_RIGHT_30_PENALTY=0.671597
PRIMER_LEFT_30_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_30_SEQUENCE=CCAGAGCTTCCCTTTTGTGT
PRIMER_LEFT_30=866,20
PRIMER_RIGHT_30=1537,20
PRIMER_LEFT_30_TM=60.363
PRIMER_RIGHT_30_TM=59.328
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=672
PRIMER_PAIR_31_PENALTY=1.034586
PRIMER_LEFT_31_PENALTY=0.362989
PRIMER_RIGHT_31_PENALTY=0.671597
PRIMER_LEFT_31_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_31_SEQUENCE=ACCAGAGCTTCCCTTTTGTG
PRIMER_LEFT_31=866,20
PRIMER_RIGHT_31=1538,20
PRIMER_LEFT_31_TM=60.363
PRIMER_RIGHT_31_TM=59.328
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=673
PRIMER_PAIR_32_PENALTY=1.042570
PRIMER_LEFT_32_PENALTY=0.362989
PRIMER_RIGHT_32_PENALTY=0.679581
PRIMER_LEFT_32_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_32_SEQUENCE=CTGTCTTGGTTTCCGACAGG
PRIMER_LEFT_32=866,20
PRIMER_RIGHT_32=1567,20
PRIMER_LEFT_32_TM=60.363
PRIMER_RIGHT_32_TM=60.680
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=702
PRIMER_PAIR_33_PENALTY=1.074173
PRIMER_LEFT_33_PENALTY=0.362989
PRIMER_RIGHT_33_PENALTY=0.711184
PRIMER_LEFT_33_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_33_SEQUENCE=TAGTCGGGTGGTCTTGCTGT
PRIMER_LEFT_33=866,20
PRIMER_RIGHT_33=1583,20
PRIMER_LEFT_33_TM=60.363
PRIMER_RIGHT_33_TM=60.711
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.6000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=718
PRIMER_PAIR_34_PENALTY=1.095002
PRIMER_LEFT_34_PENALTY=0.024663
PRIMER_RIGHT_34_PENALTY=1.070339
PRIMER_LEFT_34_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_34_SEQUENCE=CTTGAAGATGATGGTGGTGG
PRIMER_LEFT_34=773,20
PRIMER_RIGHT_34=1721,20
PRIMER_LEFT_34_TM=60.025
PRIMER_RIGHT_34_TM=58.930
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=949
PRIMER_PAIR_35_PENALTY=1.155225
PRIMER_LEFT_35_PENALTY=0.362989
PRIMER_RIGHT_35_PENALTY=0.792236
PRIMER_LEFT_35_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_35_SEQUENCE=CGTTTCTAGTCGGGTGGTCT
PRIMER_LEFT_35=866,20
PRIMER_RIGHT_35=1589,20
PRIMER_LEFT_35_TM=60.363
PRIMER_RIGHT_35_TM=59.208
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=724
PRIMER_PAIR_36_PENALTY=1.155225
PRIMER_LEFT_36_PENALTY=0.362989
PRIMER_RIGHT_36_PENALTY=0.792236
PRIMER_LEFT_36_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_36_SEQUENCE=CTCGTTTCTAGTCGGGTGGT
PRIMER_LEFT_36=866,20
PRIMER_RIGHT_36=1591,20
PRIMER_LEFT_36_TM=60.363
PRIMER_RIGHT_36_TM=59.208
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=726
PRIMER_PAIR_37_PENALTY=1.165380
PRIMER_LEFT_37_PENALTY=0.024663
PRIMER_RIGHT_37_PENALTY=1.140717
PRIMER_LEFT_37_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_37_SEQUENCE=TGGAGCTGATGCTATCGTTTT
PRIMER_LEFT_37=773,20
PRIMER_RIGHT_37=1479,21
PRIMER_LEFT_37_TM=60.025
PRIMER_RIGHT_37_TM=59.859
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=707
PRIMER_PAIR_38_PENALTY=1.165380
PRIMER_LEFT_38_PENALTY=0.024663
PRIMER_RIGHT_38_PENALTY=1.140717
PRIMER_LEFT_38_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_38_SEQUENCE=TTGGAGCTGATGCTATCGTTT
PRIMER_LEFT_38=773,20
PRIMER_RIGHT_38=1480,21
PRIMER_LEFT_38_TM=60.025
PRIMER_RIGHT_38_TM=59.859
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=8.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=708
PRIMER_PAIR_39_PENALTY=1.184796
PRIMER_LEFT_39_PENALTY=0.024663
PRIMER_RIGHT_39_PENALTY=1.160134
PRIMER_LEFT_39_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_39_SEQUENCE=ACCAGAGCTTCCCTTTTGTGT
PRIMER_LEFT_39=773,20
PRIMER_RIGHT_39=1538,21
PRIMER_LEFT_39_TM=60.025
PRIMER_RIGHT_39_TM=60.160
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=766
PRIMER_PAIR_40_PENALTY=1.189208
PRIMER_LEFT_40_PENALTY=0.024663
PRIMER_RIGHT_40_PENALTY=1.164545
PRIMER_LEFT_40_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_40_SEQUENCE=ACTTGAAGATGATGGTGGTGG
PRIMER_LEFT_40=773,20
PRIMER_RIGHT_40=1722,21
PRIMER_LEFT_40_TM=60.025
PRIMER_RIGHT_40_TM=59.835
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=9.4000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=950
PRIMER_PAIR_41_PENALTY=1.206328
PRIMER_LEFT_41_PENALTY=0.362989
PRIMER_RIGHT_41_PENALTY=0.843339
PRIMER_LEFT_41_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_41_SEQUENCE=CTAGTCGGGTGGTCTTGCTG
PRIMER_LEFT_41=866,20
PRIMER_RIGHT_41=1584,20
PRIMER_LEFT_41_TM=60.363
PRIMER_RIGHT_41_TM=60.843
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=60.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.6000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=719
PRIMER_PAIR_42_PENALTY=1.209883
PRIMER_LEFT_42_PENALTY=0.362989
PRIMER_RIGHT_42_PENALTY=0.846894
PRIMER_LEFT_42_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_42_SEQUENCE=GTTTCCGACAGGTGGTCTCT
PRIMER_LEFT_42=866,20
PRIMER_RIGHT_42=1559,20
PRIMER_LEFT_42_TM=60.363
PRIMER_RIGHT_42_TM=59.153
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=694
PRIMER_PAIR_43_PENALTY=1.275690
PRIMER_LEFT_43_PENALTY=0.362989
PRIMER_RIGHT_43_PENALTY=0.912701
PRIMER_LEFT_43_SEQUENCE=GAACTGGTGGGTGGAAGATG
PRIMER_RIGHT_43_SEQUENCE=CCACTCGTGGAGGGTATTGA
PRIMER_LEFT_43=866,20
PRIMER_RIGHT_43=1450,20
PRIMER_LEFT_43_TM=60.363
PRIMER_RIGHT_43_TM=60.913
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=585
PRIMER_PAIR_44_PENALTY=1.304878
PRIMER_LEFT_44_PENALTY=0.024663
PRIMER_RIGHT_44_PENALTY=1.280215
PRIMER_LEFT_44_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_44_SEQUENCE=TTTCCGACAGGTGGTCTCTAA
PRIMER_LEFT_44=773,20
PRIMER_RIGHT_44=1558,21
PRIMER_LEFT_44_TM=60.025
PRIMER_RIGHT_44_TM=59.720
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=6.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=786
PRIMER_PAIR_45_PENALTY=1.308386
PRIMER_LEFT_45_PENALTY=0.024663
PRIMER_RIGHT_45_PENALTY=1.283723
PRIMER_LEFT_45_SEQUENCE=CTCGTCGTCGTCAAAACTCA
PRIMER_RIGHT_45_SEQUENCE=TGGTCTTGCTGTCTTGGTTTC
PRIMER_LEFT_45=773,20
PRIMER_RIGHT_45=1575,21
PRIMER_LEFT_45_TM=60.025
PRIMER_RIGHT_45_TM=60.284
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=803
PRIMER_PAIR_46_PENALTY=1.336689
PRIMER_LEFT_46_PENALTY=0.832993
PRIMER_RIGHT_46_PENALTY=0.503696
PRIMER_LEFT_46_SEQUENCE=CCTCGTCGTCGTCAAAACTC
PRIMER_RIGHT_46_SEQUENCE=AGCTCGTTTCTAGTCGGGTG
PRIMER_LEFT_46=772,20
PRIMER_RIGHT_46=1593,20
PRIMER_LEFT_46_TM=60.833
PRIMER_RIGHT_46_TM=59.496
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=822
PRIMER_PAIR_47_PENALTY=1.336689
PRIMER_LEFT_47_PENALTY=0.832993
PRIMER_RIGHT_47_PENALTY=0.503696
PRIMER_LEFT_47_SEQUENCE=TCCTCGTCGTCGTCAAAACT
PRIMER_RIGHT_47_SEQUENCE=AGCTCGTTTCTAGTCGGGTG
PRIMER_LEFT_47=771,20
PRIMER_RIGHT_47=1593,20
PRIMER_LEFT_47_TM=60.833
PRIMER_RIGHT_47_TM=59.496
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=823
PRIMER_PAIR_48_PENALTY=1.336689
PRIMER_LEFT_48_PENALTY=0.832993
PRIMER_RIGHT_48_PENALTY=0.503696
PRIMER_LEFT_48_SEQUENCE=CTCCTCGTCGTCGTCAAAAC
PRIMER_RIGHT_48_SEQUENCE=AGCTCGTTTCTAGTCGGGTG
PRIMER_LEFT_48=770,20
PRIMER_RIGHT_48=1593,20
PRIMER_LEFT_48_TM=60.833
PRIMER_RIGHT_48_TM=59.496
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=824
PRIMER_PAIR_49_PENALTY=1.361854
PRIMER_LEFT_49_PENALTY=0.832993
PRIMER_RIGHT_49_PENALTY=0.528861
PRIMER_LEFT_49_SEQUENCE=CCTCGTCGTCGTCAAAACTC
PRIMER_RIGHT_49_SEQUENCE=TGAGCTCGTTTCTAGTCGGG
PRIMER_LEFT_49=772,20
PRIMER_RIGHT_49=1595,20
PRIMER_LEFT_49_TM=60.833
PRIMER_RIGHT_49_TM=60.529
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=11.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=824
=
SEQUENCE_ID=MAGIC.105_4_10227638_10227676_sf-2_zu-0_66333_46538_TRUE
SEQUENCE_TARGET=950,138
SEQUENCE_TEMPLATE=GTAGATGCTAGCACCGGCGAACTGAGATTCGGTGTTGATATTGAAAGTATCGTAACCAGCCTGAGCCATCTCACCATAATGTATGATATATTCCCGGAGATCTTGATCCAACGGCTGTANCATCCCTTTCCAATGGTTTTGACCGCTCAGATCTCGCCATCTCTTCGCGAATTCTCTAGNCACTATCAACTTCTCCTCCTCCTCCTCTTTCTTCTTCCTCTTCATGATTCTCTTGAAGCTAAAACACTTCATCACTTTCTTGTCNTCTTCCTCCATAATCTTTAAGATACCTNTAATGAATNTTTTNTTTAGGTTAATTAGGATCAGAGAAGCTCCTTGGATCGAANNNNNAAAGATGAATTNTGTTGAAAAATGAAAACAATAGAGTGGTTTGATGATATAAATATTCGTTGGCGTATTGTAGGCGTGTTGCAGAGGAAGTATCGTTNTCGATATGNTATTAAAATATAAAGTATTTTTTATTTGATAAAGTTTCTTAATTGATCCGAAAAGNTGTTCTTTTAACGTTTAAGGAAAGCAATAGCTCACCTTTGCATCAACATACACAGTACAAACGATTCCTTTNTCTTCTTGTAATNTTTTTTTCACTTTTTGCCATGCTGCAAATGGTTTAACCAAACCTAAAAGCGGTTCAATGTCAAATTTACTCAAATTTGAAGATATATTTTATAAGGAATCAACAGTGAAACTAATTTTGTACCGAACCTTACCAAACTAGGATTATTTTAAATTTGATTATTCCTATATTTTGTTAAACCGTAAAGTCAAAATTCGAATGAAATTACGAATCGAGCATATGTACTTTAAAAATAGTCCATATAAAAACATTAATGTATATTGCTATGAGATTGAATCATCATGTTACGTGAAAGCATTATAGTGTGGTCATGTCACTAATTGAGTAATTGTCAATTGCTTGTAAACGTGGAGCAAGCTATCTAACGTAATACTATATGTTTGGATAGGTCGATACAGCTTTNTCTTCTTTGGAATAATATCATATATTCGTTTTTGTTANAGAAAACCATTAAAAGTGATTTGCAACAAAATTTATACAAATAATTTAACATTATCTCAGATAATATAGTCGATAATGAGCCAGGTANAGCTCCGAAATTGACGGTCATGTCATTTATGTGNTTACCAACTANAAAACCCACATATTGNTACATTACATGATTTCATTTTCAGATCACAAATATAGANTATTTTGTTTTTAATTAGTATTTTCTTAGTAGTGGACGCTTCGTAACCAATGGAGGTCTACTAATTACAACCCTGCATTTATTATATCACGTTATGCATATAAAAGCTGTGAAACAAATCTAGATGATTAAATATAGCGGATTANTGGGNAAAATTAAATAATAAATTGTTNGATACACTAATCTGNAGATTTACATATTTTATTGGGTACTAAATCNTTTATGTTCAAACTAAACCATGAAGATAGGCCTTTTTACAAAATGGGCTTGAAGAGNNNNTCTTTCGTCCATATAGGCCTAANNNCTNATNTACTTGTTNNTACGNCTCTCNTACACAANGATTAGATGATTCTCAATTTGACCGNCACAATATTCGGTGAAATTTTTGATNATTTGTTGTACATGGTACATATGATTGTACAGCTGCAGAAATCCAAATGTACAACAGTACGTAATAATTGCTAATGTTAAAGTATGAACATTTTAGTGAAANNGGTCCTGAGTCCAATGCATGGTNNNNACACGAGGCTCCATTTCACTACTGTCTCCACAAAAACGAACGGTCACATACAGAGACGTGTAANTGTAGATGCACACAGAGATTGTGTAATACNTTCACCNATAGGGTTTGTCGAAATGACTAATATATAAAATATTTAAAAGCTAAACGTTTATCTATAGCAAACAACAACAAAAATAGAGTTAAAAAGTATAGTCCAAAACTTGAAATGAAGATTTGGCTATTAGATTAATAGGGTTGGTACATTAATGACTGATGGCTTATAGATAGATATGGATACCATTTCATTTTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.087848
PRIMER_LEFT_0_PENALTY=0.102638
PRIMER_RIGHT_0_PENALTY=1.985210
PRIMER_LEFT_0_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_0_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_0=635,20
PRIMER_RIGHT_0=1278,20
PRIMER_LEFT_0_TM=60.103
PRIMER_RIGHT_0_TM=61.985
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=644
PRIMER_PAIR_1_PENALTY=3.033310
PRIMER_LEFT_1_PENALTY=1.048100
PRIMER_RIGHT_1_PENALTY=1.985210
PRIMER_LEFT_1_SEQUENCE=ATTGTAGGCGTGTTGCAGAG
PRIMER_RIGHT_1_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_1=417,20
PRIMER_RIGHT_1=1278,20
PRIMER_LEFT_1_TM=58.952
PRIMER_RIGHT_1_TM=61.985
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=862
PRIMER_PAIR_2_PENALTY=3.088713
PRIMER_LEFT_2_PENALTY=1.103503
PRIMER_RIGHT_2_PENALTY=1.985210
PRIMER_LEFT_2_SEQUENCE=GCGTGTTGCAGAGGAAGTATC
PRIMER_RIGHT_2_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_2=424,21
PRIMER_RIGHT_2=1278,20
PRIMER_LEFT_2_TM=59.896
PRIMER_RIGHT_2_TM=61.985
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=855
PRIMER_PAIR_3_PENALTY=3.143357
PRIMER_LEFT_3_PENALTY=0.102638
PRIMER_RIGHT_3_PENALTY=3.040719
PRIMER_LEFT_3_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_3_SEQUENCE=CGAAGCGTCCACTACTAAGAAAA
PRIMER_LEFT_3=635,20
PRIMER_RIGHT_3=1269,23
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=59.959
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=635
PRIMER_PAIR_4_PENALTY=3.237976
PRIMER_LEFT_4_PENALTY=2.709115
PRIMER_RIGHT_4_PENALTY=0.528861
PRIMER_LEFT_4_SEQUENCE=TCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_4_SEQUENCE=CCGTTCGTTTTTGTGGAGAC
PRIMER_LEFT_4=929,20
PRIMER_RIGHT_4=1788,20
PRIMER_LEFT_4_TM=57.291
PRIMER_RIGHT_4_TM=60.529
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=860
PRIMER_PAIR_5_PENALTY=3.237976
PRIMER_LEFT_5_PENALTY=2.709115
PRIMER_RIGHT_5_PENALTY=0.528861
PRIMER_LEFT_5_SEQUENCE=TCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_5_SEQUENCE=ACCGTTCGTTTTTGTGGAGA
PRIMER_LEFT_5=929,20
PRIMER_RIGHT_5=1789,20
PRIMER_LEFT_5_TM=57.291
PRIMER_RIGHT_5_TM=60.529
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=861
PRIMER_PAIR_6_PENALTY=3.237976
PRIMER_LEFT_6_PENALTY=2.709115
PRIMER_RIGHT_6_PENALTY=0.528861
PRIMER_LEFT_6_SEQUENCE=TCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_6_SEQUENCE=GACCGTTCGTTTTTGTGGAG
PRIMER_LEFT_6=929,20
PRIMER_RIGHT_6=1790,20
PRIMER_LEFT_6_TM=57.291
PRIMER_RIGHT_6_TM=60.529
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=862
PRIMER_PAIR_7_PENALTY=3.295698
PRIMER_LEFT_7_PENALTY=2.709115
PRIMER_RIGHT_7_PENALTY=0.586583
PRIMER_LEFT_7_SEQUENCE=TCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_7_SEQUENCE=TGTGACCGTTCGTTTTTGTG
PRIMER_LEFT_7=929,20
PRIMER_RIGHT_7=1793,20
PRIMER_LEFT_7_TM=57.291
PRIMER_RIGHT_7_TM=60.587
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.9000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=865
PRIMER_PAIR_8_PENALTY=3.315045
PRIMER_LEFT_8_PENALTY=2.786184
PRIMER_RIGHT_8_PENALTY=0.528861
PRIMER_LEFT_8_SEQUENCE=GTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_8_SEQUENCE=CCGTTCGTTTTTGTGGAGAC
PRIMER_LEFT_8=928,21
PRIMER_RIGHT_8=1788,20
PRIMER_LEFT_8_TM=58.214
PRIMER_RIGHT_8_TM=60.529
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.9000
PRIMER_RIGHT_8_END_STABILITY=6.1000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=861
PRIMER_PAIR_9_PENALTY=3.315045
PRIMER_LEFT_9_PENALTY=2.786184
PRIMER_RIGHT_9_PENALTY=0.528861
PRIMER_LEFT_9_SEQUENCE=GTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_9_SEQUENCE=ACCGTTCGTTTTTGTGGAGA
PRIMER_LEFT_9=928,21
PRIMER_RIGHT_9=1789,20
PRIMER_LEFT_9_TM=58.214
PRIMER_RIGHT_9_TM=60.529
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=862
PRIMER_PAIR_10_PENALTY=3.315045
PRIMER_LEFT_10_PENALTY=2.786184
PRIMER_RIGHT_10_PENALTY=0.528861
PRIMER_LEFT_10_SEQUENCE=GTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_10_SEQUENCE=GACCGTTCGTTTTTGTGGAG
PRIMER_LEFT_10=928,21
PRIMER_RIGHT_10=1790,20
PRIMER_LEFT_10_TM=58.214
PRIMER_RIGHT_10_TM=60.529
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=863
PRIMER_PAIR_11_PENALTY=3.360038
PRIMER_LEFT_11_PENALTY=1.374827
PRIMER_RIGHT_11_PENALTY=1.985210
PRIMER_LEFT_11_SEQUENCE=GTAGGCGTGTTGCAGAGGAA
PRIMER_RIGHT_11_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_11=420,20
PRIMER_RIGHT_11=1278,20
PRIMER_LEFT_11_TM=61.375
PRIMER_RIGHT_11_TM=61.985
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=859
PRIMER_PAIR_12_PENALTY=3.372768
PRIMER_LEFT_12_PENALTY=2.786184
PRIMER_RIGHT_12_PENALTY=0.586583
PRIMER_LEFT_12_SEQUENCE=GTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_12_SEQUENCE=TGTGACCGTTCGTTTTTGTG
PRIMER_LEFT_12=928,21
PRIMER_RIGHT_12=1793,20
PRIMER_LEFT_12_TM=58.214
PRIMER_RIGHT_12_TM=60.587
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=866
PRIMER_PAIR_13_PENALTY=3.381438
PRIMER_LEFT_13_PENALTY=0.102638
PRIMER_RIGHT_13_PENALTY=3.278800
PRIMER_LEFT_13_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_13_SEQUENCE=CGAAGCGTCCACTACTAAGAAA
PRIMER_LEFT_13=635,20
PRIMER_RIGHT_13=1269,22
PRIMER_LEFT_13_TM=60.103
PRIMER_RIGHT_13_TM=58.721
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=635
PRIMER_PAIR_14_PENALTY=3.448168
PRIMER_LEFT_14_PENALTY=1.462957
PRIMER_RIGHT_14_PENALTY=1.985210
PRIMER_LEFT_14_SEQUENCE=GGCGTATTGTAGGCGTGTTG
PRIMER_RIGHT_14_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_14=412,20
PRIMER_RIGHT_14=1278,20
PRIMER_LEFT_14_TM=61.463
PRIMER_RIGHT_14_TM=61.985
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=867
PRIMER_PAIR_15_PENALTY=3.448168
PRIMER_LEFT_15_PENALTY=1.462957
PRIMER_RIGHT_15_PENALTY=1.985210
PRIMER_LEFT_15_SEQUENCE=TGGCGTATTGTAGGCGTGTT
PRIMER_RIGHT_15_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_15=411,20
PRIMER_RIGHT_15=1278,20
PRIMER_LEFT_15_TM=61.463
PRIMER_RIGHT_15_TM=61.985
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=868
PRIMER_PAIR_16_PENALTY=3.448168
PRIMER_LEFT_16_PENALTY=1.462957
PRIMER_RIGHT_16_PENALTY=1.985210
PRIMER_LEFT_16_SEQUENCE=TTGGCGTATTGTAGGCGTGT
PRIMER_RIGHT_16_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_16=410,20
PRIMER_RIGHT_16=1278,20
PRIMER_LEFT_16_TM=61.463
PRIMER_RIGHT_16_TM=61.985
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=869
PRIMER_PAIR_17_PENALTY=3.448168
PRIMER_LEFT_17_PENALTY=1.462957
PRIMER_RIGHT_17_PENALTY=1.985210
PRIMER_LEFT_17_SEQUENCE=GTTGGCGTATTGTAGGCGTG
PRIMER_RIGHT_17_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_17=409,20
PRIMER_RIGHT_17=1278,20
PRIMER_LEFT_17_TM=61.463
PRIMER_RIGHT_17_TM=61.985
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=870
PRIMER_PAIR_18_PENALTY=3.471758
PRIMER_LEFT_18_PENALTY=2.942897
PRIMER_RIGHT_18_PENALTY=0.528861
PRIMER_LEFT_18_SEQUENCE=TGTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_18_SEQUENCE=CCGTTCGTTTTTGTGGAGAC
PRIMER_LEFT_18=927,22
PRIMER_RIGHT_18=1788,20
PRIMER_LEFT_18_TM=60.943
PRIMER_RIGHT_18_TM=60.529
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=862
PRIMER_PAIR_19_PENALTY=3.471758
PRIMER_LEFT_19_PENALTY=2.942897
PRIMER_RIGHT_19_PENALTY=0.528861
PRIMER_LEFT_19_SEQUENCE=TGTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_19_SEQUENCE=ACCGTTCGTTTTTGTGGAGA
PRIMER_LEFT_19=927,22
PRIMER_RIGHT_19=1789,20
PRIMER_LEFT_19_TM=60.943
PRIMER_RIGHT_19_TM=60.529
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=863
PRIMER_PAIR_20_PENALTY=3.471758
PRIMER_LEFT_20_PENALTY=2.942897
PRIMER_RIGHT_20_PENALTY=0.528861
PRIMER_LEFT_20_SEQUENCE=TGTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_20_SEQUENCE=GACCGTTCGTTTTTGTGGAG
PRIMER_LEFT_20=927,22
PRIMER_RIGHT_20=1790,20
PRIMER_LEFT_20_TM=60.943
PRIMER_RIGHT_20_TM=60.529
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.9000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=864
PRIMER_PAIR_21_PENALTY=3.484697
PRIMER_LEFT_21_PENALTY=1.499486
PRIMER_RIGHT_21_PENALTY=1.985210
PRIMER_LEFT_21_SEQUENCE=TAGGCGTGTTGCAGAGGAAG
PRIMER_RIGHT_21_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_21=421,20
PRIMER_RIGHT_21=1278,20
PRIMER_LEFT_21_TM=61.499
PRIMER_RIGHT_21_TM=61.985
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=858
PRIMER_PAIR_22_PENALTY=3.529481
PRIMER_LEFT_22_PENALTY=2.942897
PRIMER_RIGHT_22_PENALTY=0.586583
PRIMER_LEFT_22_SEQUENCE=TGTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_22_SEQUENCE=TGTGACCGTTCGTTTTTGTG
PRIMER_LEFT_22=927,22
PRIMER_RIGHT_22=1793,20
PRIMER_LEFT_22_TM=60.943
PRIMER_RIGHT_22_TM=60.587
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.9000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=867
PRIMER_PAIR_23_PENALTY=3.598059
PRIMER_LEFT_23_PENALTY=0.102638
PRIMER_RIGHT_23_PENALTY=3.495421
PRIMER_LEFT_23_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_23_SEQUENCE=ACGAAGCGTCCACTACTAAGAAA
PRIMER_LEFT_23=635,20
PRIMER_RIGHT_23=1270,23
PRIMER_LEFT_23_TM=60.103
PRIMER_RIGHT_23_TM=59.505
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=636
PRIMER_PAIR_24_PENALTY=3.690792
PRIMER_LEFT_24_PENALTY=0.102638
PRIMER_RIGHT_24_PENALTY=3.588155
PRIMER_LEFT_24_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_24_SEQUENCE=AGGCCTATATGGACGAAAGA
PRIMER_LEFT_24=635,20
PRIMER_RIGHT_24=1524,20
PRIMER_LEFT_24_TM=60.103
PRIMER_RIGHT_24_TM=56.412
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=890
PRIMER_PAIR_25_PENALTY=3.848124
PRIMER_LEFT_25_PENALTY=1.862914
PRIMER_RIGHT_25_PENALTY=1.985210
PRIMER_LEFT_25_SEQUENCE=ACCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_25_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_25=634,21
PRIMER_RIGHT_25=1278,20
PRIMER_LEFT_25_TM=60.863
PRIMER_RIGHT_25_TM=61.985
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=645
PRIMER_PAIR_26_PENALTY=3.982187
PRIMER_LEFT_26_PENALTY=1.996977
PRIMER_RIGHT_26_PENALTY=1.985210
PRIMER_LEFT_26_SEQUENCE=TATTGTAGGCGTGTTGCAGAG
PRIMER_RIGHT_26_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_26=416,21
PRIMER_RIGHT_26=1278,20
PRIMER_LEFT_26_TM=59.003
PRIMER_RIGHT_26_TM=61.985
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=863
PRIMER_PAIR_27_PENALTY=4.071307
PRIMER_LEFT_27_PENALTY=2.709115
PRIMER_RIGHT_27_PENALTY=1.362192
PRIMER_LEFT_27_SEQUENCE=TCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_27_SEQUENCE=GTGACCGTTCGTTTTTGTGG
PRIMER_LEFT_27=929,20
PRIMER_RIGHT_27=1792,20
PRIMER_LEFT_27_TM=57.291
PRIMER_RIGHT_27_TM=61.362
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=864
PRIMER_PAIR_28_PENALTY=4.088819
PRIMER_LEFT_28_PENALTY=1.048100
PRIMER_RIGHT_28_PENALTY=3.040719
PRIMER_LEFT_28_SEQUENCE=ATTGTAGGCGTGTTGCAGAG
PRIMER_RIGHT_28_SEQUENCE=CGAAGCGTCCACTACTAAGAAAA
PRIMER_LEFT_28=417,20
PRIMER_RIGHT_28=1269,23
PRIMER_LEFT_28_TM=58.952
PRIMER_RIGHT_28_TM=59.959
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=853
PRIMER_PAIR_29_PENALTY=4.144222
PRIMER_LEFT_29_PENALTY=1.103503
PRIMER_RIGHT_29_PENALTY=3.040719
PRIMER_LEFT_29_SEQUENCE=GCGTGTTGCAGAGGAAGTATC
PRIMER_RIGHT_29_SEQUENCE=CGAAGCGTCCACTACTAAGAAAA
PRIMER_LEFT_29=424,21
PRIMER_RIGHT_29=1269,23
PRIMER_LEFT_29_TM=59.896
PRIMER_RIGHT_29_TM=59.959
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=43.478
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=5.3000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=846
PRIMER_PAIR_30_PENALTY=4.148377
PRIMER_LEFT_30_PENALTY=2.786184
PRIMER_RIGHT_30_PENALTY=1.362192
PRIMER_LEFT_30_SEQUENCE=GTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_30_SEQUENCE=GTGACCGTTCGTTTTTGTGG
PRIMER_LEFT_30=928,21
PRIMER_RIGHT_30=1792,20
PRIMER_LEFT_30_TM=58.214
PRIMER_RIGHT_30_TM=61.362
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.9000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=865
PRIMER_PAIR_31_PENALTY=4.150697
PRIMER_LEFT_31_PENALTY=2.165486
PRIMER_RIGHT_31_PENALTY=1.985210
PRIMER_LEFT_31_SEQUENCE=GTATTGTAGGCGTGTTGCAGAG
PRIMER_RIGHT_31_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_31=415,22
PRIMER_RIGHT_31=1278,20
PRIMER_LEFT_31_TM=59.835
PRIMER_RIGHT_31_TM=61.985
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=864
PRIMER_PAIR_32_PENALTY=4.209943
PRIMER_LEFT_32_PENALTY=2.709115
PRIMER_RIGHT_32_PENALTY=1.500828
PRIMER_LEFT_32_SEQUENCE=TCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_32_SEQUENCE=CCATGCATTGGACTCAGGAC
PRIMER_LEFT_32=929,20
PRIMER_RIGHT_32=1740,20
PRIMER_LEFT_32_TM=57.291
PRIMER_RIGHT_32_TM=61.501
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.9000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=812
PRIMER_PAIR_33_PENALTY=4.275952
PRIMER_LEFT_33_PENALTY=0.102638
PRIMER_RIGHT_33_PENALTY=4.173314
PRIMER_LEFT_33_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_33_SEQUENCE=CGAAGCGTCCACTACTAAGA
PRIMER_LEFT_33=635,20
PRIMER_RIGHT_33=1269,20
PRIMER_LEFT_33_TM=60.103
PRIMER_RIGHT_33_TM=55.827
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=635
PRIMER_PAIR_34_PENALTY=4.287013
PRIMER_LEFT_34_PENALTY=2.786184
PRIMER_RIGHT_34_PENALTY=1.500828
PRIMER_LEFT_34_SEQUENCE=GTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_34_SEQUENCE=CCATGCATTGGACTCAGGAC
PRIMER_LEFT_34=928,21
PRIMER_RIGHT_34=1740,20
PRIMER_LEFT_34_TM=58.214
PRIMER_RIGHT_34_TM=61.501
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.9000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=813
PRIMER_PAIR_35_PENALTY=4.294860
PRIMER_LEFT_35_PENALTY=0.102638
PRIMER_RIGHT_35_PENALTY=4.192222
PRIMER_LEFT_35_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_35_SEQUENCE=ACGAAGCGTCCACTACTAAGA
PRIMER_LEFT_35=635,20
PRIMER_RIGHT_35=1270,21
PRIMER_LEFT_35_TM=60.103
PRIMER_RIGHT_35_TM=56.808
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=6.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=636
PRIMER_PAIR_36_PENALTY=4.305090
PRIMER_LEFT_36_PENALTY=2.942897
PRIMER_RIGHT_36_PENALTY=1.362192
PRIMER_LEFT_36_SEQUENCE=TGTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_36_SEQUENCE=GTGACCGTTCGTTTTTGTGG
PRIMER_LEFT_36=927,22
PRIMER_RIGHT_36=1792,20
PRIMER_LEFT_36_TM=60.943
PRIMER_RIGHT_36_TM=61.362
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=866
PRIMER_PAIR_37_PENALTY=4.315789
PRIMER_LEFT_37_PENALTY=2.330579
PRIMER_RIGHT_37_PENALTY=1.985210
PRIMER_LEFT_37_SEQUENCE=AGGCGTGTTGCAGAGGAAGT
PRIMER_RIGHT_37_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_37=422,20
PRIMER_RIGHT_37=1278,20
PRIMER_LEFT_37_TM=62.331
PRIMER_RIGHT_37_TM=61.985
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=857
PRIMER_PAIR_38_PENALTY=4.322974
PRIMER_LEFT_38_PENALTY=2.337764
PRIMER_RIGHT_38_PENALTY=1.985210
PRIMER_LEFT_38_SEQUENCE=ATTCGTTGGCGTATTGTAGG
PRIMER_RIGHT_38_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_38=405,20
PRIMER_RIGHT_38=1278,20
PRIMER_LEFT_38_TM=57.662
PRIMER_RIGHT_38_TM=61.985
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=874
PRIMER_PAIR_39_PENALTY=4.326900
PRIMER_LEFT_39_PENALTY=1.048100
PRIMER_RIGHT_39_PENALTY=3.278800
PRIMER_LEFT_39_SEQUENCE=ATTGTAGGCGTGTTGCAGAG
PRIMER_RIGHT_39_SEQUENCE=CGAAGCGTCCACTACTAAGAAA
PRIMER_LEFT_39=417,20
PRIMER_RIGHT_39=1269,22
PRIMER_LEFT_39_TM=58.952
PRIMER_RIGHT_39_TM=58.721
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=853
PRIMER_PAIR_40_PENALTY=4.331881
PRIMER_LEFT_40_PENALTY=2.346670
PRIMER_RIGHT_40_PENALTY=1.985210
PRIMER_LEFT_40_SEQUENCE=TTGTAGGCGTGTTGCAGAGG
PRIMER_RIGHT_40_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_40=418,20
PRIMER_RIGHT_40=1278,20
PRIMER_LEFT_40_TM=62.347
PRIMER_RIGHT_40_TM=61.985
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=861
PRIMER_PAIR_41_PENALTY=4.342505
PRIMER_LEFT_41_PENALTY=2.357294
PRIMER_RIGHT_41_PENALTY=1.985210
PRIMER_LEFT_41_SEQUENCE=GCTCACCTTTGCATCAACATAC
PRIMER_RIGHT_41_SEQUENCE=CATTGGTTACGAAGCGTCCA
PRIMER_LEFT_41=543,22
PRIMER_RIGHT_41=1278,20
PRIMER_LEFT_41_TM=59.643
PRIMER_RIGHT_41_TM=61.985
PRIMER_LEFT_41_GC_PERCENT=45.455
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=5.6000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=736
PRIMER_PAIR_42_PENALTY=4.347293
PRIMER_LEFT_42_PENALTY=0.102638
PRIMER_RIGHT_42_PENALTY=4.244655
PRIMER_LEFT_42_SEQUENCE=CCAAACCTAAAAGCGGTTCA
PRIMER_RIGHT_42_SEQUENCE=GGCCTATCTTCATGGTTTAGTTTG
PRIMER_LEFT_42=635,20
PRIMER_RIGHT_42=1476,24
PRIMER_LEFT_42_TM=60.103
PRIMER_RIGHT_42_TM=60.245
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=41.667
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=842
PRIMER_PAIR_43_PENALTY=4.382303
PRIMER_LEFT_43_PENALTY=1.103503
PRIMER_RIGHT_43_PENALTY=3.278800
PRIMER_LEFT_43_SEQUENCE=GCGTGTTGCAGAGGAAGTATC
PRIMER_RIGHT_43_SEQUENCE=CGAAGCGTCCACTACTAAGAAA
PRIMER_LEFT_43=424,21
PRIMER_RIGHT_43=1269,22
PRIMER_LEFT_43_TM=59.896
PRIMER_RIGHT_43_TM=58.721
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=5.3000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=846
PRIMER_PAIR_44_PENALTY=4.414542
PRIMER_LEFT_44_PENALTY=2.709115
PRIMER_RIGHT_44_PENALTY=1.705427
PRIMER_LEFT_44_SEQUENCE=TCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_44_SEQUENCE=CGTTCGTTTTTGTGGAGACAG
PRIMER_LEFT_44=929,20
PRIMER_RIGHT_44=1787,21
PRIMER_LEFT_44_TM=57.291
PRIMER_RIGHT_44_TM=60.705
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.9000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=859
PRIMER_PAIR_45_PENALTY=4.415546
PRIMER_LEFT_45_PENALTY=1.374827
PRIMER_RIGHT_45_PENALTY=3.040719
PRIMER_LEFT_45_SEQUENCE=GTAGGCGTGTTGCAGAGGAA
PRIMER_RIGHT_45_SEQUENCE=CGAAGCGTCCACTACTAAGAAAA
PRIMER_LEFT_45=420,20
PRIMER_RIGHT_45=1269,23
PRIMER_LEFT_45_TM=61.375
PRIMER_RIGHT_45_TM=59.959
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=43.478
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=850
PRIMER_PAIR_46_PENALTY=4.443726
PRIMER_LEFT_46_PENALTY=2.942897
PRIMER_RIGHT_46_PENALTY=1.500828
PRIMER_LEFT_46_SEQUENCE=TGTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_46_SEQUENCE=CCATGCATTGGACTCAGGAC
PRIMER_LEFT_46=927,22
PRIMER_RIGHT_46=1740,20
PRIMER_LEFT_46_TM=60.943
PRIMER_RIGHT_46_TM=61.501
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=814
PRIMER_PAIR_47_PENALTY=4.491612
PRIMER_LEFT_47_PENALTY=2.786184
PRIMER_RIGHT_47_PENALTY=1.705427
PRIMER_LEFT_47_SEQUENCE=GTCAATTGCTTGTAAACGTGG
PRIMER_RIGHT_47_SEQUENCE=CGTTCGTTTTTGTGGAGACAG
PRIMER_LEFT_47=928,21
PRIMER_RIGHT_47=1787,21
PRIMER_LEFT_47_TM=58.214
PRIMER_RIGHT_47_TM=60.705
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=860
PRIMER_PAIR_48_PENALTY=4.503676
PRIMER_LEFT_48_PENALTY=1.462957
PRIMER_RIGHT_48_PENALTY=3.040719
PRIMER_LEFT_48_SEQUENCE=GGCGTATTGTAGGCGTGTTG
PRIMER_RIGHT_48_SEQUENCE=CGAAGCGTCCACTACTAAGAAAA
PRIMER_LEFT_48=412,20
PRIMER_RIGHT_48=1269,23
PRIMER_LEFT_48_TM=61.463
PRIMER_RIGHT_48_TM=59.959
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=43.478
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=858
PRIMER_PAIR_49_PENALTY=4.503676
PRIMER_LEFT_49_PENALTY=1.462957
PRIMER_RIGHT_49_PENALTY=3.040719
PRIMER_LEFT_49_SEQUENCE=TGGCGTATTGTAGGCGTGTT
PRIMER_RIGHT_49_SEQUENCE=CGAAGCGTCCACTACTAAGAAAA
PRIMER_LEFT_49=411,20
PRIMER_RIGHT_49=1269,23
PRIMER_LEFT_49_TM=61.463
PRIMER_RIGHT_49_TM=59.959
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=43.478
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=859
=
SEQUENCE_ID=MAGIC.446_4_4741819_4741920_can-0_bur-0_2734_31834_TRUE
SEQUENCE_TARGET=950,201
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNATTCTACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_8335583_8335887_po-0_zu-0_305111_74629_TRUE
SEQUENCE_TARGET=950,404
SEQUENCE_TEMPLATE=AATATGTTGATNAGAATAGTAAACCCCATCGCTTGTTGACCAAGTTATTTGGTGGCTTAGGANTGTTGNGTGGATTGNTTTGGTGAAACTTTGTGTANTTTTAAGTGTGTTGTTCTGCTTGAATACTTTAACATTGGTCGACTAAGTTATTTTGTGTGTTGGGNTNGTNAAGTGGATTCCTTNTGTGCNATTTTTTGAGCTTTCTAANTGTGTTCTGCTTNAANACNNNNTAAACGTAGGGAAGGATTTGGAGACATTATTCTTGCTTTCAGAATGCAANCAAAGACACAAAGATTGANTTTTNATTTATAAGGAGNGGATCACGTATACTAGANGNNNTGAAGTGTGAAATGTTACCCCTTTCTCTGAAAAGATCCAAGACGAAGTTGCCTCCCTGTCGATGATCTCAATGAGTCACCAACATCAGTGGATTCAAGAACATATAATAACTATANATNTAACTTCAAGAAGAAATTACANNNNNAGAAAACAAATGCACCTTCTTGGTGTGTTATAGAAAATATAATGTTTAGTGTTCATTCCNGTAATGTNNGTTGGTATGACTCAGATCTTAGAAAATGGTACAGAGTTTCNGGATTGANATATCTGCNTATGTCACATCTTGTTAGNTATCATACCAANTGTGNGGGAGATTNANTTTTTACATGTTGTGGTTACTACTGTCCCAATTTNATNTCACTTGTTGAGCTGNTATGACCCTTTGAACAAACAAAGGATAAANAATCTCACCTTATCTTGGCTTNGGGGNCAGAACAATTCTCAAGANAATTNAAGCTNNTTATTTCTTANGAATGGGGTTCAAGTTTGGCNNNNNNNNNNNNNNNNNNNNTTNAANGTTATTTAGCTTGANTTGTCTATGTTATNAGGCTGAGACNNTTTTTNTNTTAATGGAAGATAGNATAATANACACTTCGATTATTTTACAAAGCAAACANAACTCAACNTTNTTCTTCTTCTTCATCACACTNCACACNACACNNAACTTCCAAGTTTANGGAAAATGAAATANTCGGATGAATTCAAAGATCNNNNNATTTACCTTGCTTGNGGAAACATTCCCGAATCAAAATTCGTCATNGCNGGNTCCGTGACACTACTGGCGCCAGAACCACCACCGGCTCTTGCTCTGCTTACACTCTCCTTTAGCTCAGCCACTACATGACTCATCGTCGGTCTATTCTTAGAACTAGAAGATGCACAAGCCATTGCTACTTCAGTGATCTTCCATGCCNAACCAGCATCAAACCTCTCNCCTAGCTTTGGATCCACAATGCCTCTGATATNTCCTGTCGACAGCATTAAGTCGACCCGGTCAGTTATGTGTATGTTCTCTGCTGTTGTTCTTGAACGTGCAATCACCGGTTGGCCTGNGACAACCTCTAAAAGAACCACCCCGAAGCTGTAAANATCACTCTTCTCGCTTAATTTTTNCGNCAAATGGTACCTTCATTTTNTAAAACAATGCCTNAGAANCTNTAACAGCTNATACCAAATGACAATGGAAGAAGCTTTTTACTTACTCNGGATCGAGATAACCGATNGTTCCAGCAACANCGGTTGTATCCTGGTTATTACCGTCTAGTGCGACACTTCTAGATAATCCAAAGTCAGCAATCTTGGCCTNGAGCTTCTCNTTGATTAGAATATTAGCCGGCTTCACATCTCTTTGTACAATGGGAGGCTTGCAGCCATTGTGAAGATACTCAAGCCCTGAGATTCAAACAAACAACANANTTTTATTTCATTGANTTTNTCACNTACCTCTTTGTGTATTCGTTACCAACCTTGTGCCGCGTCTAGTGATATTTGTAACCTCTCTTCCCAGCTCAAGACATAAGATTTTTCCCCTTGATTTTGAAAGAAACCANAAGGTAAGTTTAGTTTCCTCTNGCNACAGCANCAAAANACCANTNANNAAAACTAACTACCAGAGAGACTNACCTGACAAGTAATCTCCNAAGGTTCCATTAGCCATAAACTCATAGATAAGCGCCATCTTCTTGCCTTCGTGGCAGTATCCGATAAGAGCGGTTAGGTTCTTGTGATGGACTCTCAGGAGAAGTTCAACCTGATGATAACAAGTGAATGCATAATTGATTACTCCATAANTCANTNATTAAANACTGATGTATAAGAGACTTACCTCTGCTCTGAACTCTTTGTAACCTTGAGCTGATGATTCAGACAGAATCTTGACAGCGACTTGGTCATCATTTAAGACACCATGATATACTTTTCCAAAACCTCCTTGTCCAAGAACTCTCTCAAAGTTGTTTGTAACTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.679722
PRIMER_LEFT_0_PENALTY=1.145871
PRIMER_RIGHT_0_PENALTY=1.533851
PRIMER_LEFT_0_SEQUENCE=TGAGTCACCAACATCAGTGGA
PRIMER_RIGHT_0_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_0=410,21
PRIMER_RIGHT_0=1374,20
PRIMER_LEFT_0_TM=60.146
PRIMER_RIGHT_0_TM=58.466
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=965
PRIMER_PAIR_1_PENALTY=2.685391
PRIMER_LEFT_1_PENALTY=1.377118
PRIMER_RIGHT_1_PENALTY=1.308273
PRIMER_LEFT_1_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_1_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_1=491,20
PRIMER_RIGHT_1=1419,20
PRIMER_LEFT_1_TM=58.623
PRIMER_RIGHT_1_TM=58.692
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=929
PRIMER_PAIR_2_PENALTY=2.685391
PRIMER_LEFT_2_PENALTY=1.377118
PRIMER_RIGHT_2_PENALTY=1.308273
PRIMER_LEFT_2_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_2_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_2=490,20
PRIMER_RIGHT_2=1419,20
PRIMER_LEFT_2_TM=58.623
PRIMER_RIGHT_2_TM=58.692
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=930
PRIMER_PAIR_3_PENALTY=2.797567
PRIMER_LEFT_3_PENALTY=1.489294
PRIMER_RIGHT_3_PENALTY=1.308273
PRIMER_LEFT_3_SEQUENCE=AAATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_3_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_3=491,21
PRIMER_RIGHT_3=1419,20
PRIMER_LEFT_3_TM=59.511
PRIMER_RIGHT_3_TM=58.692
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=929
PRIMER_PAIR_4_PENALTY=2.797567
PRIMER_LEFT_4_PENALTY=1.489294
PRIMER_RIGHT_4_PENALTY=1.308273
PRIMER_LEFT_4_SEQUENCE=ACAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_4_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_4=489,21
PRIMER_RIGHT_4=1419,20
PRIMER_LEFT_4_TM=59.511
PRIMER_RIGHT_4_TM=58.692
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=931
PRIMER_PAIR_5_PENALTY=2.910969
PRIMER_LEFT_5_PENALTY=1.377118
PRIMER_RIGHT_5_PENALTY=1.533851
PRIMER_LEFT_5_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_5_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_5=491,20
PRIMER_RIGHT_5=1374,20
PRIMER_LEFT_5_TM=58.623
PRIMER_RIGHT_5_TM=58.466
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=884
PRIMER_PAIR_6_PENALTY=2.910969
PRIMER_LEFT_6_PENALTY=1.377118
PRIMER_RIGHT_6_PENALTY=1.533851
PRIMER_LEFT_6_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_6_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_6=490,20
PRIMER_RIGHT_6=1374,20
PRIMER_LEFT_6_TM=58.623
PRIMER_RIGHT_6_TM=58.466
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=885
PRIMER_PAIR_7_PENALTY=3.023145
PRIMER_LEFT_7_PENALTY=1.489294
PRIMER_RIGHT_7_PENALTY=1.533851
PRIMER_LEFT_7_SEQUENCE=AAATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_7_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_7=491,21
PRIMER_RIGHT_7=1374,20
PRIMER_LEFT_7_TM=59.511
PRIMER_RIGHT_7_TM=58.466
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=884
PRIMER_PAIR_8_PENALTY=3.023145
PRIMER_LEFT_8_PENALTY=1.489294
PRIMER_RIGHT_8_PENALTY=1.533851
PRIMER_LEFT_8_SEQUENCE=ACAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_8_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_8=489,21
PRIMER_RIGHT_8=1374,20
PRIMER_LEFT_8_TM=59.511
PRIMER_RIGHT_8_TM=58.466
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=886
PRIMER_PAIR_9_PENALTY=3.144114
PRIMER_LEFT_9_PENALTY=1.377118
PRIMER_RIGHT_9_PENALTY=1.766996
PRIMER_LEFT_9_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_9_SEQUENCE=TTTACAGCTTCGGGGTGGTT
PRIMER_LEFT_9=491,20
PRIMER_RIGHT_9=1426,20
PRIMER_LEFT_9_TM=58.623
PRIMER_RIGHT_9_TM=61.767
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=936
PRIMER_PAIR_10_PENALTY=3.144114
PRIMER_LEFT_10_PENALTY=1.377118
PRIMER_RIGHT_10_PENALTY=1.766996
PRIMER_LEFT_10_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_10_SEQUENCE=TTTACAGCTTCGGGGTGGTT
PRIMER_LEFT_10=490,20
PRIMER_RIGHT_10=1426,20
PRIMER_LEFT_10_TM=58.623
PRIMER_RIGHT_10_TM=61.767
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=937
PRIMER_PAIR_11_PENALTY=3.244894
PRIMER_LEFT_11_PENALTY=1.711043
PRIMER_RIGHT_11_PENALTY=1.533851
PRIMER_LEFT_11_SEQUENCE=AAGACGAAGTTGCCTCCCTG
PRIMER_RIGHT_11_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_11=377,20
PRIMER_RIGHT_11=1374,20
PRIMER_LEFT_11_TM=61.711
PRIMER_RIGHT_11_TM=58.466
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=998
PRIMER_PAIR_12_PENALTY=3.244894
PRIMER_LEFT_12_PENALTY=1.711043
PRIMER_RIGHT_12_PENALTY=1.533851
PRIMER_LEFT_12_SEQUENCE=CAAGACGAAGTTGCCTCCCT
PRIMER_RIGHT_12_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_12=376,20
PRIMER_RIGHT_12=1374,20
PRIMER_LEFT_12_TM=61.711
PRIMER_RIGHT_12_TM=58.466
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=999
PRIMER_PAIR_13_PENALTY=3.250125
PRIMER_LEFT_13_PENALTY=1.941852
PRIMER_RIGHT_13_PENALTY=1.308273
PRIMER_LEFT_13_SEQUENCE=ATGCACCTTCTTGGTGTGTT
PRIMER_RIGHT_13_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_13=493,20
PRIMER_RIGHT_13=1419,20
PRIMER_LEFT_13_TM=58.058
PRIMER_RIGHT_13_TM=58.692
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=927
PRIMER_PAIR_14_PENALTY=3.250125
PRIMER_LEFT_14_PENALTY=1.941852
PRIMER_RIGHT_14_PENALTY=1.308273
PRIMER_LEFT_14_SEQUENCE=AATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_14_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_14=492,20
PRIMER_RIGHT_14=1419,20
PRIMER_LEFT_14_TM=58.058
PRIMER_RIGHT_14_TM=58.692
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=6.3000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=928
PRIMER_PAIR_15_PENALTY=3.256290
PRIMER_LEFT_15_PENALTY=1.489294
PRIMER_RIGHT_15_PENALTY=1.766996
PRIMER_LEFT_15_SEQUENCE=AAATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_15_SEQUENCE=TTTACAGCTTCGGGGTGGTT
PRIMER_LEFT_15=491,21
PRIMER_RIGHT_15=1426,20
PRIMER_LEFT_15_TM=59.511
PRIMER_RIGHT_15_TM=61.767
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=936
PRIMER_PAIR_16_PENALTY=3.256290
PRIMER_LEFT_16_PENALTY=1.489294
PRIMER_RIGHT_16_PENALTY=1.766996
PRIMER_LEFT_16_SEQUENCE=ACAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_16_SEQUENCE=TTTACAGCTTCGGGGTGGTT
PRIMER_LEFT_16=489,21
PRIMER_RIGHT_16=1426,20
PRIMER_LEFT_16_TM=59.511
PRIMER_RIGHT_16_TM=61.767
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=938
PRIMER_PAIR_17_PENALTY=3.335637
PRIMER_LEFT_17_PENALTY=1.377118
PRIMER_RIGHT_17_PENALTY=1.958519
PRIMER_LEFT_17_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_17_SEQUENCE=TACAGCTTCGGGGTGGTTCT
PRIMER_LEFT_17=491,20
PRIMER_RIGHT_17=1424,20
PRIMER_LEFT_17_TM=58.623
PRIMER_RIGHT_17_TM=61.959
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=934
PRIMER_PAIR_18_PENALTY=3.335637
PRIMER_LEFT_18_PENALTY=1.377118
PRIMER_RIGHT_18_PENALTY=1.958519
PRIMER_LEFT_18_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_18_SEQUENCE=TACAGCTTCGGGGTGGTTCT
PRIMER_LEFT_18=490,20
PRIMER_RIGHT_18=1424,20
PRIMER_LEFT_18_TM=58.623
PRIMER_RIGHT_18_TM=61.959
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=935
PRIMER_PAIR_19_PENALTY=3.447813
PRIMER_LEFT_19_PENALTY=1.489294
PRIMER_RIGHT_19_PENALTY=1.958519
PRIMER_LEFT_19_SEQUENCE=AAATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_19_SEQUENCE=TACAGCTTCGGGGTGGTTCT
PRIMER_LEFT_19=491,21
PRIMER_RIGHT_19=1424,20
PRIMER_LEFT_19_TM=59.511
PRIMER_RIGHT_19_TM=61.959
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=934
PRIMER_PAIR_20_PENALTY=3.447813
PRIMER_LEFT_20_PENALTY=1.489294
PRIMER_RIGHT_20_PENALTY=1.958519
PRIMER_LEFT_20_SEQUENCE=ACAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_20_SEQUENCE=TACAGCTTCGGGGTGGTTCT
PRIMER_LEFT_20=489,21
PRIMER_RIGHT_20=1424,20
PRIMER_LEFT_20_TM=59.511
PRIMER_RIGHT_20_TM=61.959
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=936
PRIMER_PAIR_21_PENALTY=3.475703
PRIMER_LEFT_21_PENALTY=1.941852
PRIMER_RIGHT_21_PENALTY=1.533851
PRIMER_LEFT_21_SEQUENCE=ATGCACCTTCTTGGTGTGTT
PRIMER_RIGHT_21_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_21=493,20
PRIMER_RIGHT_21=1374,20
PRIMER_LEFT_21_TM=58.058
PRIMER_RIGHT_21_TM=58.466
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=882
PRIMER_PAIR_22_PENALTY=3.475703
PRIMER_LEFT_22_PENALTY=1.941852
PRIMER_RIGHT_22_PENALTY=1.533851
PRIMER_LEFT_22_SEQUENCE=AATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_22_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_22=492,20
PRIMER_RIGHT_22=1374,20
PRIMER_LEFT_22_TM=58.058
PRIMER_RIGHT_22_TM=58.466
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=883
PRIMER_PAIR_23_PENALTY=3.535098
PRIMER_LEFT_23_PENALTY=2.001247
PRIMER_RIGHT_23_PENALTY=1.533851
PRIMER_LEFT_23_SEQUENCE=TGAGTCACCAACATCAGTGG
PRIMER_RIGHT_23_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_23=410,20
PRIMER_RIGHT_23=1374,20
PRIMER_LEFT_23_TM=57.999
PRIMER_RIGHT_23_TM=58.466
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=965
PRIMER_PAIR_24_PENALTY=3.579733
PRIMER_LEFT_24_PENALTY=2.271460
PRIMER_RIGHT_24_PENALTY=1.308273
PRIMER_LEFT_24_SEQUENCE=AAACAAATGCACCTTCTTGG
PRIMER_RIGHT_24_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_24=487,20
PRIMER_RIGHT_24=1419,20
PRIMER_LEFT_24_TM=57.729
PRIMER_RIGHT_24_TM=58.692
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=933
PRIMER_PAIR_25_PENALTY=3.589597
PRIMER_LEFT_25_PENALTY=1.377118
PRIMER_RIGHT_25_PENALTY=2.212479
PRIMER_LEFT_25_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_25_SEQUENCE=AGCTTCGGGGTGGTTCTTTT
PRIMER_LEFT_25=491,20
PRIMER_RIGHT_25=1421,20
PRIMER_LEFT_25_TM=58.623
PRIMER_RIGHT_25_TM=62.212
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=7.3000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=931
PRIMER_PAIR_26_PENALTY=3.589597
PRIMER_LEFT_26_PENALTY=1.377118
PRIMER_RIGHT_26_PENALTY=2.212479
PRIMER_LEFT_26_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_26_SEQUENCE=AGCTTCGGGGTGGTTCTTTT
PRIMER_LEFT_26=490,20
PRIMER_RIGHT_26=1421,20
PRIMER_LEFT_26_TM=58.623
PRIMER_RIGHT_26_TM=62.212
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=7.3000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=932
PRIMER_PAIR_27_PENALTY=3.643051
PRIMER_LEFT_27_PENALTY=2.109200
PRIMER_RIGHT_27_PENALTY=1.533851
PRIMER_LEFT_27_SEQUENCE=AATGAGTCACCAACATCAGTGG
PRIMER_RIGHT_27_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_27=408,22
PRIMER_RIGHT_27=1374,20
PRIMER_LEFT_27_TM=59.891
PRIMER_RIGHT_27_TM=58.466
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=967
PRIMER_PAIR_28_PENALTY=3.693473
PRIMER_LEFT_28_PENALTY=1.377118
PRIMER_RIGHT_28_PENALTY=2.316355
PRIMER_LEFT_28_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_28_SEQUENCE=TTACAGCTTCGGGGTGGTTC
PRIMER_LEFT_28=491,20
PRIMER_RIGHT_28=1425,20
PRIMER_LEFT_28_TM=58.623
PRIMER_RIGHT_28_TM=62.316
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=935
PRIMER_PAIR_29_PENALTY=3.693473
PRIMER_LEFT_29_PENALTY=1.377118
PRIMER_RIGHT_29_PENALTY=2.316355
PRIMER_LEFT_29_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_29_SEQUENCE=TTACAGCTTCGGGGTGGTTC
PRIMER_LEFT_29=490,20
PRIMER_RIGHT_29=1425,20
PRIMER_LEFT_29_TM=58.623
PRIMER_RIGHT_29_TM=62.316
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=936
PRIMER_PAIR_30_PENALTY=3.694664
PRIMER_LEFT_30_PENALTY=2.160813
PRIMER_RIGHT_30_PENALTY=1.533851
PRIMER_LEFT_30_SEQUENCE=GACGAAGTTGCCTCCCTGTC
PRIMER_RIGHT_30_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_30=379,20
PRIMER_RIGHT_30=1374,20
PRIMER_LEFT_30_TM=62.161
PRIMER_RIGHT_30_TM=58.466
PRIMER_LEFT_30_GC_PERCENT=60.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=996
PRIMER_PAIR_31_PENALTY=3.701773
PRIMER_LEFT_31_PENALTY=1.489294
PRIMER_RIGHT_31_PENALTY=2.212479
PRIMER_LEFT_31_SEQUENCE=AAATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_31_SEQUENCE=AGCTTCGGGGTGGTTCTTTT
PRIMER_LEFT_31=491,21
PRIMER_RIGHT_31=1421,20
PRIMER_LEFT_31_TM=59.511
PRIMER_RIGHT_31_TM=62.212
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=931
PRIMER_PAIR_32_PENALTY=3.701773
PRIMER_LEFT_32_PENALTY=1.489294
PRIMER_RIGHT_32_PENALTY=2.212479
PRIMER_LEFT_32_SEQUENCE=ACAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_32_SEQUENCE=AGCTTCGGGGTGGTTCTTTT
PRIMER_LEFT_32=489,21
PRIMER_RIGHT_32=1421,20
PRIMER_LEFT_32_TM=59.511
PRIMER_RIGHT_32_TM=62.212
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=933
PRIMER_PAIR_33_PENALTY=3.708848
PRIMER_LEFT_33_PENALTY=1.941852
PRIMER_RIGHT_33_PENALTY=1.766996
PRIMER_LEFT_33_SEQUENCE=ATGCACCTTCTTGGTGTGTT
PRIMER_RIGHT_33_SEQUENCE=TTTACAGCTTCGGGGTGGTT
PRIMER_LEFT_33=493,20
PRIMER_RIGHT_33=1426,20
PRIMER_LEFT_33_TM=58.058
PRIMER_RIGHT_33_TM=61.767
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=934
PRIMER_PAIR_34_PENALTY=3.708848
PRIMER_LEFT_34_PENALTY=1.941852
PRIMER_RIGHT_34_PENALTY=1.766996
PRIMER_LEFT_34_SEQUENCE=AATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_34_SEQUENCE=TTTACAGCTTCGGGGTGGTT
PRIMER_LEFT_34=492,20
PRIMER_RIGHT_34=1426,20
PRIMER_LEFT_34_TM=58.058
PRIMER_RIGHT_34_TM=61.767
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=935
PRIMER_PAIR_35_PENALTY=3.805312
PRIMER_LEFT_35_PENALTY=2.271460
PRIMER_RIGHT_35_PENALTY=1.533851
PRIMER_LEFT_35_SEQUENCE=AAACAAATGCACCTTCTTGG
PRIMER_RIGHT_35_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_35=487,20
PRIMER_RIGHT_35=1374,20
PRIMER_LEFT_35_TM=57.729
PRIMER_RIGHT_35_TM=58.466
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=888
PRIMER_PAIR_36_PENALTY=3.805649
PRIMER_LEFT_36_PENALTY=1.489294
PRIMER_RIGHT_36_PENALTY=2.316355
PRIMER_LEFT_36_SEQUENCE=AAATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_36_SEQUENCE=TTACAGCTTCGGGGTGGTTC
PRIMER_LEFT_36=491,21
PRIMER_RIGHT_36=1425,20
PRIMER_LEFT_36_TM=59.511
PRIMER_RIGHT_36_TM=62.316
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=935
PRIMER_PAIR_37_PENALTY=3.805649
PRIMER_LEFT_37_PENALTY=1.489294
PRIMER_RIGHT_37_PENALTY=2.316355
PRIMER_LEFT_37_SEQUENCE=ACAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_37_SEQUENCE=TTACAGCTTCGGGGTGGTTC
PRIMER_LEFT_37=489,21
PRIMER_RIGHT_37=1425,20
PRIMER_LEFT_37_TM=59.511
PRIMER_RIGHT_37_TM=62.316
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=937
PRIMER_PAIR_38_PENALTY=3.810669
PRIMER_LEFT_38_PENALTY=2.502397
PRIMER_RIGHT_38_PENALTY=1.308273
PRIMER_LEFT_38_SEQUENCE=CAAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_38_SEQUENCE=CTTCGGGGTGGTTCTTTTAG
PRIMER_LEFT_38=490,21
PRIMER_RIGHT_38=1419,20
PRIMER_LEFT_38_TM=61.502
PRIMER_RIGHT_38_TM=58.692
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=930
PRIMER_PAIR_39_PENALTY=3.877225
PRIMER_LEFT_39_PENALTY=1.377118
PRIMER_RIGHT_39_PENALTY=2.500107
PRIMER_LEFT_39_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_39_SEQUENCE=GGGGTGGTTCTTTTAGAGGTT
PRIMER_LEFT_39=491,20
PRIMER_RIGHT_39=1415,21
PRIMER_LEFT_39_TM=58.623
PRIMER_RIGHT_39_TM=58.500
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=925
PRIMER_PAIR_40_PENALTY=3.877225
PRIMER_LEFT_40_PENALTY=1.377118
PRIMER_RIGHT_40_PENALTY=2.500107
PRIMER_LEFT_40_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_40_SEQUENCE=GGGGTGGTTCTTTTAGAGGTT
PRIMER_LEFT_40=490,20
PRIMER_RIGHT_40=1415,21
PRIMER_LEFT_40_TM=58.623
PRIMER_RIGHT_40_TM=58.500
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=926
PRIMER_PAIR_41_PENALTY=3.900371
PRIMER_LEFT_41_PENALTY=1.941852
PRIMER_RIGHT_41_PENALTY=1.958519
PRIMER_LEFT_41_SEQUENCE=ATGCACCTTCTTGGTGTGTT
PRIMER_RIGHT_41_SEQUENCE=TACAGCTTCGGGGTGGTTCT
PRIMER_LEFT_41=493,20
PRIMER_RIGHT_41=1424,20
PRIMER_LEFT_41_TM=58.058
PRIMER_RIGHT_41_TM=61.959
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=932
PRIMER_PAIR_42_PENALTY=3.900371
PRIMER_LEFT_42_PENALTY=1.941852
PRIMER_RIGHT_42_PENALTY=1.958519
PRIMER_LEFT_42_SEQUENCE=AATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_42_SEQUENCE=TACAGCTTCGGGGTGGTTCT
PRIMER_LEFT_42=492,20
PRIMER_RIGHT_42=1424,20
PRIMER_LEFT_42_TM=58.058
PRIMER_RIGHT_42_TM=61.959
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=933
PRIMER_PAIR_43_PENALTY=3.943769
PRIMER_LEFT_43_PENALTY=2.409918
PRIMER_RIGHT_43_PENALTY=1.533851
PRIMER_LEFT_43_SEQUENCE=CACCAACATCAGTGGATTCAAG
PRIMER_RIGHT_43_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_43=415,22
PRIMER_RIGHT_43=1374,20
PRIMER_LEFT_43_TM=60.410
PRIMER_RIGHT_43_TM=58.466
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=960
PRIMER_PAIR_44_PENALTY=3.946686
PRIMER_LEFT_44_PENALTY=1.377118
PRIMER_RIGHT_44_PENALTY=2.569568
PRIMER_LEFT_44_SEQUENCE=AAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_44_SEQUENCE=CGGGGTGGTTCTTTTAGAGGT
PRIMER_LEFT_44=491,20
PRIMER_RIGHT_44=1416,21
PRIMER_LEFT_44_TM=58.623
PRIMER_RIGHT_44_TM=61.570
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=926
PRIMER_PAIR_45_PENALTY=3.946686
PRIMER_LEFT_45_PENALTY=1.377118
PRIMER_RIGHT_45_PENALTY=2.569568
PRIMER_LEFT_45_SEQUENCE=CAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_45_SEQUENCE=CGGGGTGGTTCTTTTAGAGGT
PRIMER_LEFT_45=490,20
PRIMER_RIGHT_45=1416,21
PRIMER_LEFT_45_TM=58.623
PRIMER_RIGHT_45_TM=61.570
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=927
PRIMER_PAIR_46_PENALTY=3.964777
PRIMER_LEFT_46_PENALTY=2.430926
PRIMER_RIGHT_46_PENALTY=1.533851
PRIMER_LEFT_46_SEQUENCE=ATGAGTCACCAACATCAGTGGA
PRIMER_RIGHT_46_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_46=409,22
PRIMER_RIGHT_46=1374,20
PRIMER_LEFT_46_TM=60.431
PRIMER_RIGHT_46_TM=58.466
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=966
PRIMER_PAIR_47_PENALTY=3.989401
PRIMER_LEFT_47_PENALTY=1.489294
PRIMER_RIGHT_47_PENALTY=2.500107
PRIMER_LEFT_47_SEQUENCE=AAATGCACCTTCTTGGTGTGT
PRIMER_RIGHT_47_SEQUENCE=GGGGTGGTTCTTTTAGAGGTT
PRIMER_LEFT_47=491,21
PRIMER_RIGHT_47=1415,21
PRIMER_LEFT_47_TM=59.511
PRIMER_RIGHT_47_TM=58.500
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=925
PRIMER_PAIR_48_PENALTY=3.989401
PRIMER_LEFT_48_PENALTY=1.489294
PRIMER_RIGHT_48_PENALTY=2.500107
PRIMER_LEFT_48_SEQUENCE=ACAAATGCACCTTCTTGGTGT
PRIMER_RIGHT_48_SEQUENCE=GGGGTGGTTCTTTTAGAGGTT
PRIMER_LEFT_48=489,21
PRIMER_RIGHT_48=1415,21
PRIMER_LEFT_48_TM=59.511
PRIMER_RIGHT_48_TM=58.500
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=927
PRIMER_PAIR_49_PENALTY=4.036248
PRIMER_LEFT_49_PENALTY=2.502397
PRIMER_RIGHT_49_PENALTY=1.533851
PRIMER_LEFT_49_SEQUENCE=CAAATGCACCTTCTTGGTGTG
PRIMER_RIGHT_49_SEQUENCE=TGCACGTTCAAGAACAACAG
PRIMER_LEFT_49=490,21
PRIMER_RIGHT_49=1374,20
PRIMER_LEFT_49_TM=61.502
PRIMER_RIGHT_49_TM=58.466
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=885
=
SEQUENCE_ID=MAGIC.329_4_4603881_4604061_edi-0_wil-2_29514_22767_TRUE
SEQUENCE_TARGET=950,280
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNCANATCTCTGTTCAAACATAAAGCTGTNTTAANACANGAACAANAATCNAATCTATTGAAATATNAGGAATCNTTAACANATTCANATTGNNGGTTACNNCTTTGATNAATACNAATTTGCCGNTTTAGAGATGATTNACGAANNNNNNNNNNNNNGATNCCCCNTANGAATCTGGNTTCTNCTANNACTTTGACGCAGATCTATATNTTAANAANNNAATCAAANTCTAGAANTNTANTNNACCAATTTNAAAGNACNAAAANCATAAATCCGANGNNNGAANNTAACTTACNTCTTTGTAGATCTNACAATTTGCTAACNACAAGTNTAGGATGANNANGAAATCATAAGNNNNNNNNNNNNTTTTGTGATTGAANCTTAGATCGACNGANNANNAANNNTTTTTGGNTTCGAATNATTNTTNTTTTTANAGACAACNTTNGTTNACNATNGAATANNNGTATATTAATATACAAAANTNNANAANCAATTCNNNATATAGTTNNGGCCNATNNTNACANNAAANTNGGCCNATATTACAATAAAANATNTTTNNNNNNATCTTATNNNNNNNNNNTTATATNTACNTTNTATNANCTTATGAGNTGTNAAATATGTCTTNGTGTATAAAATTTTAGATGGTTNATGTTGTTTTGTAAAAGTTTAGGTGTTACAAAANGAAAAATGACAATCATCATTGGTCAATANTGNTTAACAATCGGAAGACCATAATTCNATGTTTTTTNCCTTNNACTTTTGGGGTTTTCTACAANTTNCTATTAGTTATANNNNTAGTTTNAAAANTTTANAGCATNNAAGAATTTGANTNGAAAANGTTCAATATGTGTGGAAGTTAAACCATGTATATGTGTGAAAGTATCAATAATTGAGTGAAGATGGTGGCAACNCTGATCTTAAAACGCACAAATCTTATGAGNAAAANCAACAATGCTGNTATTAGAGGTTGNAGGAGATTTGTTTGGAACAAATTATTTGCTACTCTAAAATAAATTATTTTGAATGGTTATTCAGTANTTTTGACGAATGCAACATCAATTTCTTGTGTCCAAAAAGNNNNNNNNNNNNNNANCNTAANGAAGTGGTGGTGGCGGCGNGANTTGNGTGANCTTACATGNNNNNNNNNNNNAGAAAGCTCTCATGGGAAAAATGTCTAGACCAATAAGTTAAANTTGTTTACGTTAAACN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_5845150_5845879_edi-0_wil-2_143795_165325_TRUE
SEQUENCE_TARGET=950,829
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_4723190_4723653_po-0_wu-0_51429_328964_TRUE
SEQUENCE_TARGET=950,563
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGCAACATGATCTNTGCAGATATGTCTTATTTANNNACTGATTCTCTCTGGTATGACNGNAAACATCNTTGTATAACCTCAACTTGAAACTACTATTTTNTCTTCATCTTCTTNCTNANATGNTTCTAGTTTTGCNTAGNCCCCTTCTCTAATTTACTAGGATCNGCTTCATCTCANTTCTCCTTACTGTCACAAACGAAATCTCCATCTCTTAATGCCTTTCCTTTGNGGTGGTGATGANCTCTTCTCCTCCGCCACGTATNGTCCTTCGTCTCCTCTTCCTCTNATCTTCCTCANNNTTATCCTTCATCTCTTTNCTTTCTCTTCTCCTCTTCAANAGTTCCACAATGACTAACATACTCAACAAAAACGGTAGTGGCTGACCTNTAATTAGGGTTAAGATTAAGANGANGCNGTGACGCTCGNTTTAGGAANGGTGGCATGAATCTCTTTNNTTTCCTTCACTTGTGAACTCNCTTCTCTCTCCAATATGGTAACGACCANACTATAAAACTCATGTNTTNATGCTTTGGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_8482186_8482468_kn-0_no-0_174453_107132_TRUE
SEQUENCE_TARGET=950,382
SEQUENCE_TEMPLATE=ATAATATATGCACAAATTAGGATACGCTAAGCATGTGGATTCAGTGACTTAGTAGTTAGCACTCAAATGGTTTTATGTTTATGGCAAAAGATCCCATGNGATGCGATTAGGATTGGGTAAAACTGAACGGAAAAGATGTATTAGAGGAAGGACAAGTCAGCAACTTCCCTCGCGGGTTGTGCCGATCAGCCATTGTCTTTTCAGCANAAGGACTTGAGTGTTTATCATCATCAACCCGACTAAAAAGGAAAGACAAGTCTTCCCCAATCCGTTGATGGAGCAAAACCNGCTTTCTCCTTCCAATTTGTTTNATCCGTTTCTTGAAATAANTACTCTCGGCTTTGTTTGGGGTTTGGGATTATNTAAGGTTCTTGGGGTTTCTTTTTGTACAAAAGATCANATGTTTGGAATGGGAAGTAGAATATGTCTCTCTTGTCTCAGAGACTTTGAAAGANATTTTANTTCATAACCANTGATGTATTTACTACAGTTACAGTTTCGACTTGCAAATTGTGTTTGGATATTGGATCATGTCGTAGTTTGCATTTATAACAAGTCTTACTTGATCAACACATACCTCTTCTTTGAAACTAATTCCAACTTTTCTGATAGANATTGTTAGCATATATTAGAGACATTCGAACATGTGTTGTATACCAATTACTNAATGTCCATTTAATGTGGTTGGATATGATTTTGAAGGAAATTTTTGACTNGAGAGATCATCGTGACTTAATTATTTAGAAGGCGACTTGGGAGATCATCGTGAGACTGTTAGAGTTCTCCTCACCTAACTCACTCGAATGCTCTTTTNGGAGAATAATAATATTTAAAAGTCTTTAGTTTATTATCAAAGAATCAAAGAATCCAAACGCACAAAATTAATAGCTTAGTTTNTTATCTGATCCACATTTTCAAATNGATCTTAACATTANGCAGACGNCCATGTANNNAANCGCCTAGCCGACGCGAGTTTGCTTTTTACANTTCNGGGACTCTTNTCCNCTCTTGAGAGCCAGAGAACGCNTTTTGATATATTGACCACCACAAAGCTTACCCTTGAGAGTCAAGTCTCCTCTATGGCCCATGACTCTTCTAACTATAGCTTGGCGTTCAANATGCTCCCCAATCTCAAAAACCTGCCGAGGGGCTCGAGGCTCTGCCAAGCCCTTAAACGTTATGTAGANATGTGCCCTGTAGGCTTTGAGTCTCTCATTAGACGGCTCCTCATCTTTGGTAGCAGTTTCTATCGCCACTTTCACAATCTCCAACTNGGACAGAANTTCNGNCTTCTGAACTGNCTACAATTTTGGAGTTCATNTTATTATTATTACCAGTAGTGCCACTCGTGGTGGTATGTCATTAATTGNNATATATATATGTAAAACTTACCTCAGTGATCGACCTATCAGTTGTAGTAATTACAAGTTCCAAATACATAGAAATCCAATCAGTGGCCTGCCACTCTGATTCCTTCAGCTGCAAAANCAATAACCACATATCAATTGATCATGAGCTAGCACAACAAAANTTAAGATAATGAAGTGTCATAACATATTATAGGGCATTTTACCATGTAAAATCGTTTTCCATCATTCAAAGCATCATCTGAGGGCCAATCAGGCAATTCACCTTGGAAGATACCACCGGCCCGTGCATACAAAGCATCATCTAAGACATGCTCAGGCAATTCATCTTTGGAGACAGCCTCCATCAGTGCATTCAAAGCATCATCTGAGAGACAATCAGGCAATTCACCTTTGAATATACCATCGCCCAATGCGCCACCATGAAAGTGAGGTATGAAGGGCGTNTTGGTGGACACTATTCAACATTCATTATTAATCAGCAAGCTAAGGGAGATTGTTATAGTCAGGCTAAAATTTTATAAAGAAAACAAGTCACCTTCAAGTAAGNCACCTTCGTGTTTAGGTCTAGCAATGGAGCAGTTTATACCCAAANTGCCAAATTGTTGTTCATCAACTCGAACCTGAAAGGTTTTCTGCGATGAGCTAGCAGCTGGATCGGGATCATGTGCAAGCAGAGTCATGTAGAAAGAGGACATATAGTTTTGTAACATGTTGAACTTCATTAAGGCAGCGAGCTCGAAGCTTGTTCCCTGACAGATGAAAGAAGAAAATCGAAACAATCAATGTGGTATATATAACAGAGCANACGTTTATAACATACCAANTAGATGATACAAAGAGTTTAAATATTTACCTTGATCAAATTGTAACGATGAAGCCCAAACTTAGCATAAAGTTTGACCAAGACAGGAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.119110
PRIMER_LEFT_0_PENALTY=0.051674
PRIMER_RIGHT_0_PENALTY=0.067436
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_0_SEQUENCE=AAGGTGAATTGCCTGATTGG
PRIMER_LEFT_0=740,20
PRIMER_RIGHT_0=1634,20
PRIMER_LEFT_0_TM=59.948
PRIMER_RIGHT_0_TM=59.933
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=895
PRIMER_PAIR_1_PENALTY=0.119110
PRIMER_LEFT_1_PENALTY=0.051674
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_1_SEQUENCE=CCAAGGTGAATTGCCTGATT
PRIMER_LEFT_1=740,20
PRIMER_RIGHT_1=1636,20
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=897
PRIMER_PAIR_2_PENALTY=0.246272
PRIMER_LEFT_2_PENALTY=0.051674
PRIMER_RIGHT_2_PENALTY=0.194598
PRIMER_LEFT_2_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_2_SEQUENCE=GCTTTGAATGCACTGATGGA
PRIMER_LEFT_2=740,20
PRIMER_RIGHT_2=1728,20
PRIMER_LEFT_2_TM=59.948
PRIMER_RIGHT_2_TM=59.805
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=989
PRIMER_PAIR_3_PENALTY=0.248577
PRIMER_LEFT_3_PENALTY=0.051674
PRIMER_RIGHT_3_PENALTY=0.196903
PRIMER_LEFT_3_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_3_SEQUENCE=GAAGGAATCAGAGTGGCAGG
PRIMER_LEFT_3=740,20
PRIMER_RIGHT_3=1476,20
PRIMER_LEFT_3_TM=59.948
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=737
PRIMER_PAIR_4_PENALTY=0.301815
PRIMER_LEFT_4_PENALTY=0.234379
PRIMER_RIGHT_4_PENALTY=0.067436
PRIMER_LEFT_4_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_4_SEQUENCE=AAGGTGAATTGCCTGATTGG
PRIMER_LEFT_4=858,20
PRIMER_RIGHT_4=1634,20
PRIMER_LEFT_4_TM=60.234
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=777
PRIMER_PAIR_5_PENALTY=0.301815
PRIMER_LEFT_5_PENALTY=0.234379
PRIMER_RIGHT_5_PENALTY=0.067436
PRIMER_LEFT_5_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_5_SEQUENCE=CCAAGGTGAATTGCCTGATT
PRIMER_LEFT_5=858,20
PRIMER_RIGHT_5=1636,20
PRIMER_LEFT_5_TM=60.234
PRIMER_RIGHT_5_TM=59.933
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=779
PRIMER_PAIR_6_PENALTY=0.361726
PRIMER_LEFT_6_PENALTY=0.294290
PRIMER_RIGHT_6_PENALTY=0.067436
PRIMER_LEFT_6_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_6_SEQUENCE=CCAAGGTGAATTGCCTGATT
PRIMER_LEFT_6=859,20
PRIMER_RIGHT_6=1636,20
PRIMER_LEFT_6_TM=59.706
PRIMER_RIGHT_6_TM=59.933
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=778
PRIMER_PAIR_7_PENALTY=0.403484
PRIMER_LEFT_7_PENALTY=0.051674
PRIMER_RIGHT_7_PENALTY=0.351810
PRIMER_LEFT_7_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_7_SEQUENCE=TTTGAATGCACTGATGGAGG
PRIMER_LEFT_7=740,20
PRIMER_RIGHT_7=1726,20
PRIMER_LEFT_7_TM=59.948
PRIMER_RIGHT_7_TM=59.648
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=987
PRIMER_PAIR_8_PENALTY=0.428977
PRIMER_LEFT_8_PENALTY=0.234379
PRIMER_RIGHT_8_PENALTY=0.194598
PRIMER_LEFT_8_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_8_SEQUENCE=GCTTTGAATGCACTGATGGA
PRIMER_LEFT_8=858,20
PRIMER_RIGHT_8=1728,20
PRIMER_LEFT_8_TM=60.234
PRIMER_RIGHT_8_TM=59.805
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=871
PRIMER_PAIR_9_PENALTY=0.431282
PRIMER_LEFT_9_PENALTY=0.234379
PRIMER_RIGHT_9_PENALTY=0.196903
PRIMER_LEFT_9_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_9_SEQUENCE=GAAGGAATCAGAGTGGCAGG
PRIMER_LEFT_9=858,20
PRIMER_RIGHT_9=1476,20
PRIMER_LEFT_9_TM=60.234
PRIMER_RIGHT_9_TM=59.803
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=619
PRIMER_PAIR_10_PENALTY=0.488888
PRIMER_LEFT_10_PENALTY=0.294290
PRIMER_RIGHT_10_PENALTY=0.194598
PRIMER_LEFT_10_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_10_SEQUENCE=GCTTTGAATGCACTGATGGA
PRIMER_LEFT_10=859,20
PRIMER_RIGHT_10=1728,20
PRIMER_LEFT_10_TM=59.706
PRIMER_RIGHT_10_TM=59.805
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=870
PRIMER_PAIR_11_PENALTY=0.491193
PRIMER_LEFT_11_PENALTY=0.294290
PRIMER_RIGHT_11_PENALTY=0.196903
PRIMER_LEFT_11_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_11_SEQUENCE=GAAGGAATCAGAGTGGCAGG
PRIMER_LEFT_11=859,20
PRIMER_RIGHT_11=1476,20
PRIMER_LEFT_11_TM=59.706
PRIMER_RIGHT_11_TM=59.803
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=618
PRIMER_PAIR_12_PENALTY=0.509395
PRIMER_LEFT_12_PENALTY=0.051674
PRIMER_RIGHT_12_PENALTY=0.457721
PRIMER_LEFT_12_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_12_SEQUENCE=TTTTGCAGCTGAAGGAATCA
PRIMER_LEFT_12=740,20
PRIMER_RIGHT_12=1486,20
PRIMER_LEFT_12_TM=59.948
PRIMER_RIGHT_12_TM=59.542
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=747
PRIMER_PAIR_13_PENALTY=0.586189
PRIMER_LEFT_13_PENALTY=0.234379
PRIMER_RIGHT_13_PENALTY=0.351810
PRIMER_LEFT_13_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_13_SEQUENCE=TTTGAATGCACTGATGGAGG
PRIMER_LEFT_13=858,20
PRIMER_RIGHT_13=1726,20
PRIMER_LEFT_13_TM=60.234
PRIMER_RIGHT_13_TM=59.648
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=869
PRIMER_PAIR_14_PENALTY=0.619719
PRIMER_LEFT_14_PENALTY=0.552282
PRIMER_RIGHT_14_PENALTY=0.067436
PRIMER_LEFT_14_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_14_SEQUENCE=AAGGTGAATTGCCTGATTGG
PRIMER_LEFT_14=739,20
PRIMER_RIGHT_14=1634,20
PRIMER_LEFT_14_TM=59.448
PRIMER_RIGHT_14_TM=59.933
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=896
PRIMER_PAIR_15_PENALTY=0.619719
PRIMER_LEFT_15_PENALTY=0.552282
PRIMER_RIGHT_15_PENALTY=0.067436
PRIMER_LEFT_15_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_15_SEQUENCE=CCAAGGTGAATTGCCTGATT
PRIMER_LEFT_15=739,20
PRIMER_RIGHT_15=1636,20
PRIMER_LEFT_15_TM=59.448
PRIMER_RIGHT_15_TM=59.933
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=898
PRIMER_PAIR_16_PENALTY=0.646100
PRIMER_LEFT_16_PENALTY=0.294290
PRIMER_RIGHT_16_PENALTY=0.351810
PRIMER_LEFT_16_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_16_SEQUENCE=TTTGAATGCACTGATGGAGG
PRIMER_LEFT_16=859,20
PRIMER_RIGHT_16=1726,20
PRIMER_LEFT_16_TM=59.706
PRIMER_RIGHT_16_TM=59.648
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=868
PRIMER_PAIR_17_PENALTY=0.692100
PRIMER_LEFT_17_PENALTY=0.234379
PRIMER_RIGHT_17_PENALTY=0.457721
PRIMER_LEFT_17_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_17_SEQUENCE=TTTTGCAGCTGAAGGAATCA
PRIMER_LEFT_17=858,20
PRIMER_RIGHT_17=1486,20
PRIMER_LEFT_17_TM=60.234
PRIMER_RIGHT_17_TM=59.542
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=629
PRIMER_PAIR_18_PENALTY=0.740063
PRIMER_LEFT_18_PENALTY=0.051674
PRIMER_RIGHT_18_PENALTY=0.688390
PRIMER_LEFT_18_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_18_SEQUENCE=AATGCACTGATGGAGGCTGT
PRIMER_LEFT_18=740,20
PRIMER_RIGHT_18=1722,20
PRIMER_LEFT_18_TM=59.948
PRIMER_RIGHT_18_TM=60.688
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=2.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=983
PRIMER_PAIR_19_PENALTY=0.746881
PRIMER_LEFT_19_PENALTY=0.552282
PRIMER_RIGHT_19_PENALTY=0.194598
PRIMER_LEFT_19_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_19_SEQUENCE=GCTTTGAATGCACTGATGGA
PRIMER_LEFT_19=739,20
PRIMER_RIGHT_19=1728,20
PRIMER_LEFT_19_TM=59.448
PRIMER_RIGHT_19_TM=59.805
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=990
PRIMER_PAIR_20_PENALTY=0.749186
PRIMER_LEFT_20_PENALTY=0.552282
PRIMER_RIGHT_20_PENALTY=0.196903
PRIMER_LEFT_20_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_20_SEQUENCE=GAAGGAATCAGAGTGGCAGG
PRIMER_LEFT_20=739,20
PRIMER_RIGHT_20=1476,20
PRIMER_LEFT_20_TM=59.448
PRIMER_RIGHT_20_TM=59.803
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=738
PRIMER_PAIR_21_PENALTY=0.752011
PRIMER_LEFT_21_PENALTY=0.294290
PRIMER_RIGHT_21_PENALTY=0.457721
PRIMER_LEFT_21_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_21_SEQUENCE=TTTTGCAGCTGAAGGAATCA
PRIMER_LEFT_21=859,20
PRIMER_RIGHT_21=1486,20
PRIMER_LEFT_21_TM=59.706
PRIMER_RIGHT_21_TM=59.542
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=628
PRIMER_PAIR_22_PENALTY=0.784844
PRIMER_LEFT_22_PENALTY=0.051674
PRIMER_RIGHT_22_PENALTY=0.733171
PRIMER_LEFT_22_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_22_SEQUENCE=TGCAGCTGAAGGAATCAGAG
PRIMER_LEFT_22=740,20
PRIMER_RIGHT_22=1483,20
PRIMER_LEFT_22_TM=59.948
PRIMER_RIGHT_22_TM=59.267
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=744
PRIMER_PAIR_23_PENALTY=0.904093
PRIMER_LEFT_23_PENALTY=0.552282
PRIMER_RIGHT_23_PENALTY=0.351810
PRIMER_LEFT_23_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_23_SEQUENCE=TTTGAATGCACTGATGGAGG
PRIMER_LEFT_23=739,20
PRIMER_RIGHT_23=1726,20
PRIMER_LEFT_23_TM=59.448
PRIMER_RIGHT_23_TM=59.648
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=0.922768
PRIMER_LEFT_24_PENALTY=0.234379
PRIMER_RIGHT_24_PENALTY=0.688390
PRIMER_LEFT_24_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_24_SEQUENCE=AATGCACTGATGGAGGCTGT
PRIMER_LEFT_24=858,20
PRIMER_RIGHT_24=1722,20
PRIMER_LEFT_24_TM=60.234
PRIMER_RIGHT_24_TM=60.688
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=865
PRIMER_PAIR_25_PENALTY=0.928709
PRIMER_LEFT_25_PENALTY=0.051674
PRIMER_RIGHT_25_PENALTY=0.877035
PRIMER_LEFT_25_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_25_SEQUENCE=CAAGGTGAATTGCCTGATTG
PRIMER_LEFT_25=740,20
PRIMER_RIGHT_25=1635,20
PRIMER_LEFT_25_TM=59.948
PRIMER_RIGHT_25_TM=59.123
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=896
PRIMER_PAIR_26_PENALTY=0.967549
PRIMER_LEFT_26_PENALTY=0.234379
PRIMER_RIGHT_26_PENALTY=0.733171
PRIMER_LEFT_26_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_26_SEQUENCE=TGCAGCTGAAGGAATCAGAG
PRIMER_LEFT_26=858,20
PRIMER_RIGHT_26=1483,20
PRIMER_LEFT_26_TM=60.234
PRIMER_RIGHT_26_TM=59.267
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=626
PRIMER_PAIR_27_PENALTY=0.982679
PRIMER_LEFT_27_PENALTY=0.294290
PRIMER_RIGHT_27_PENALTY=0.688390
PRIMER_LEFT_27_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_27_SEQUENCE=AATGCACTGATGGAGGCTGT
PRIMER_LEFT_27=859,20
PRIMER_RIGHT_27=1722,20
PRIMER_LEFT_27_TM=59.706
PRIMER_RIGHT_27_TM=60.688
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=864
PRIMER_PAIR_28_PENALTY=0.987431
PRIMER_LEFT_28_PENALTY=0.234379
PRIMER_RIGHT_28_PENALTY=0.753052
PRIMER_LEFT_28_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_28_SEQUENCE=TTCAAAGGTGAATTGCCTGA
PRIMER_LEFT_28=858,20
PRIMER_RIGHT_28=1764,20
PRIMER_LEFT_28_TM=60.234
PRIMER_RIGHT_28_TM=59.247
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=907
PRIMER_PAIR_29_PENALTY=1.010004
PRIMER_LEFT_29_PENALTY=0.552282
PRIMER_RIGHT_29_PENALTY=0.457721
PRIMER_LEFT_29_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_29_SEQUENCE=TTTTGCAGCTGAAGGAATCA
PRIMER_LEFT_29=739,20
PRIMER_RIGHT_29=1486,20
PRIMER_LEFT_29_TM=59.448
PRIMER_RIGHT_29_TM=59.542
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=748
PRIMER_PAIR_30_PENALTY=1.027460
PRIMER_LEFT_30_PENALTY=0.294290
PRIMER_RIGHT_30_PENALTY=0.733171
PRIMER_LEFT_30_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_30_SEQUENCE=TGCAGCTGAAGGAATCAGAG
PRIMER_LEFT_30=859,20
PRIMER_RIGHT_30=1483,20
PRIMER_LEFT_30_TM=59.706
PRIMER_RIGHT_30_TM=59.267
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=625
PRIMER_PAIR_31_PENALTY=1.033914
PRIMER_LEFT_31_PENALTY=0.234379
PRIMER_RIGHT_31_PENALTY=0.799535
PRIMER_LEFT_31_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_31_SEQUENCE=TCCAAAGATGAATTGCCTGA
PRIMER_LEFT_31=858,20
PRIMER_RIGHT_31=1701,20
PRIMER_LEFT_31_TM=60.234
PRIMER_RIGHT_31_TM=59.200
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=844
PRIMER_PAIR_32_PENALTY=1.039854
PRIMER_LEFT_32_PENALTY=0.234379
PRIMER_RIGHT_32_PENALTY=0.805475
PRIMER_LEFT_32_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_32_SEQUENCE=CGCCCTTCATACCTCACTTT
PRIMER_LEFT_32=858,20
PRIMER_RIGHT_32=1811,20
PRIMER_LEFT_32_TM=60.234
PRIMER_RIGHT_32_TM=59.195
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=954
PRIMER_PAIR_33_PENALTY=1.047342
PRIMER_LEFT_33_PENALTY=0.294290
PRIMER_RIGHT_33_PENALTY=0.753052
PRIMER_LEFT_33_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_33_SEQUENCE=TTCAAAGGTGAATTGCCTGA
PRIMER_LEFT_33=859,20
PRIMER_RIGHT_33=1764,20
PRIMER_LEFT_33_TM=59.706
PRIMER_RIGHT_33_TM=59.247
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=906
PRIMER_PAIR_34_PENALTY=1.054305
PRIMER_LEFT_34_PENALTY=0.051674
PRIMER_RIGHT_34_PENALTY=1.002631
PRIMER_LEFT_34_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_34_SEQUENCE=CTGAAGGAATCAGAGTGGCAG
PRIMER_LEFT_34=740,20
PRIMER_RIGHT_34=1478,21
PRIMER_LEFT_34_TM=59.948
PRIMER_RIGHT_34_TM=59.997
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=739
PRIMER_PAIR_35_PENALTY=1.054305
PRIMER_LEFT_35_PENALTY=0.051674
PRIMER_RIGHT_35_PENALTY=1.002631
PRIMER_LEFT_35_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_35_SEQUENCE=CAGCTGAAGGAATCAGAGTGG
PRIMER_LEFT_35=740,20
PRIMER_RIGHT_35=1481,21
PRIMER_LEFT_35_TM=59.948
PRIMER_RIGHT_35_TM=59.997
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=742
PRIMER_PAIR_36_PENALTY=1.093825
PRIMER_LEFT_36_PENALTY=0.294290
PRIMER_RIGHT_36_PENALTY=0.799535
PRIMER_LEFT_36_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_36_SEQUENCE=TCCAAAGATGAATTGCCTGA
PRIMER_LEFT_36=859,20
PRIMER_RIGHT_36=1701,20
PRIMER_LEFT_36_TM=59.706
PRIMER_RIGHT_36_TM=59.200
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=843
PRIMER_PAIR_37_PENALTY=1.094952
PRIMER_LEFT_37_PENALTY=0.051674
PRIMER_RIGHT_37_PENALTY=1.043279
PRIMER_LEFT_37_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_37_SEQUENCE=TGAAGGAATCAGAGTGGCAG
PRIMER_LEFT_37=740,20
PRIMER_RIGHT_37=1477,20
PRIMER_LEFT_37_TM=59.948
PRIMER_RIGHT_37_TM=58.957
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=738
PRIMER_PAIR_38_PENALTY=1.099765
PRIMER_LEFT_38_PENALTY=0.294290
PRIMER_RIGHT_38_PENALTY=0.805475
PRIMER_LEFT_38_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_38_SEQUENCE=CGCCCTTCATACCTCACTTT
PRIMER_LEFT_38=859,20
PRIMER_RIGHT_38=1811,20
PRIMER_LEFT_38_TM=59.706
PRIMER_RIGHT_38_TM=59.195
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=953
PRIMER_PAIR_39_PENALTY=1.111414
PRIMER_LEFT_39_PENALTY=0.234379
PRIMER_RIGHT_39_PENALTY=0.877035
PRIMER_LEFT_39_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_39_SEQUENCE=CAAGGTGAATTGCCTGATTG
PRIMER_LEFT_39=858,20
PRIMER_RIGHT_39=1635,20
PRIMER_LEFT_39_TM=60.234
PRIMER_RIGHT_39_TM=59.123
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=778
PRIMER_PAIR_40_PENALTY=1.171325
PRIMER_LEFT_40_PENALTY=0.294290
PRIMER_RIGHT_40_PENALTY=0.877035
PRIMER_LEFT_40_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_40_SEQUENCE=CAAGGTGAATTGCCTGATTG
PRIMER_LEFT_40=859,20
PRIMER_RIGHT_40=1635,20
PRIMER_LEFT_40_TM=59.706
PRIMER_RIGHT_40_TM=59.123
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=777
PRIMER_PAIR_41_PENALTY=1.200597
PRIMER_LEFT_41_PENALTY=0.051674
PRIMER_RIGHT_41_PENALTY=1.148923
PRIMER_LEFT_41_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_41_SEQUENCE=GCAGCTGAAGGAATCAGAGTG
PRIMER_LEFT_41=740,20
PRIMER_RIGHT_41=1482,21
PRIMER_LEFT_41_TM=59.948
PRIMER_RIGHT_41_TM=60.149
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=743
PRIMER_PAIR_42_PENALTY=1.237010
PRIMER_LEFT_42_PENALTY=0.234379
PRIMER_RIGHT_42_PENALTY=1.002631
PRIMER_LEFT_42_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_42_SEQUENCE=CTGAAGGAATCAGAGTGGCAG
PRIMER_LEFT_42=858,20
PRIMER_RIGHT_42=1478,21
PRIMER_LEFT_42_TM=60.234
PRIMER_RIGHT_42_TM=59.997
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=9.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=621
PRIMER_PAIR_43_PENALTY=1.237010
PRIMER_LEFT_43_PENALTY=0.234379
PRIMER_RIGHT_43_PENALTY=1.002631
PRIMER_LEFT_43_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_43_SEQUENCE=CAGCTGAAGGAATCAGAGTGG
PRIMER_LEFT_43=858,20
PRIMER_RIGHT_43=1481,21
PRIMER_LEFT_43_TM=60.234
PRIMER_RIGHT_43_TM=59.997
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=624
PRIMER_PAIR_44_PENALTY=1.240672
PRIMER_LEFT_44_PENALTY=0.552282
PRIMER_RIGHT_44_PENALTY=0.688390
PRIMER_LEFT_44_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_44_SEQUENCE=AATGCACTGATGGAGGCTGT
PRIMER_LEFT_44=739,20
PRIMER_RIGHT_44=1722,20
PRIMER_LEFT_44_TM=59.448
PRIMER_RIGHT_44_TM=60.688
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=2.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=984
PRIMER_PAIR_45_PENALTY=1.245998
PRIMER_LEFT_45_PENALTY=0.234379
PRIMER_RIGHT_45_PENALTY=1.011619
PRIMER_LEFT_45_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_45_SEQUENCE=ACGCCCTTCATACCTCACTTT
PRIMER_LEFT_45=858,20
PRIMER_RIGHT_45=1812,21
PRIMER_LEFT_45_TM=60.234
PRIMER_RIGHT_45_TM=60.012
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=955
PRIMER_PAIR_46_PENALTY=1.250388
PRIMER_LEFT_46_PENALTY=0.051674
PRIMER_RIGHT_46_PENALTY=1.198715
PRIMER_LEFT_46_SEQUENCE=TTAGAAGGCGACTTGGGAGA
PRIMER_RIGHT_46_SEQUENCE=TCTCCAAAGATGAATTGCCTG
PRIMER_LEFT_46=740,20
PRIMER_RIGHT_46=1703,21
PRIMER_LEFT_46_TM=59.948
PRIMER_RIGHT_46_TM=60.199
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=9.7000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=964
PRIMER_PAIR_47_PENALTY=1.277657
PRIMER_LEFT_47_PENALTY=0.234379
PRIMER_RIGHT_47_PENALTY=1.043279
PRIMER_LEFT_47_SEQUENCE=TCAAAGAATCCAAACGCACA
PRIMER_RIGHT_47_SEQUENCE=TGAAGGAATCAGAGTGGCAG
PRIMER_LEFT_47=858,20
PRIMER_RIGHT_47=1477,20
PRIMER_LEFT_47_TM=60.234
PRIMER_RIGHT_47_TM=58.957
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=620
PRIMER_PAIR_48_PENALTY=1.285453
PRIMER_LEFT_48_PENALTY=0.552282
PRIMER_RIGHT_48_PENALTY=0.733171
PRIMER_LEFT_48_SEQUENCE=TTTAGAAGGCGACTTGGGAG
PRIMER_RIGHT_48_SEQUENCE=TGCAGCTGAAGGAATCAGAG
PRIMER_LEFT_48=739,20
PRIMER_RIGHT_48=1483,20
PRIMER_LEFT_48_TM=59.448
PRIMER_RIGHT_48_TM=59.267
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=745
PRIMER_PAIR_49_PENALTY=1.296921
PRIMER_LEFT_49_PENALTY=0.294290
PRIMER_RIGHT_49_PENALTY=1.002631
PRIMER_LEFT_49_SEQUENCE=CAAAGAATCCAAACGCACAA
PRIMER_RIGHT_49_SEQUENCE=CTGAAGGAATCAGAGTGGCAG
PRIMER_LEFT_49=859,20
PRIMER_RIGHT_49=1478,21
PRIMER_LEFT_49_TM=59.706
PRIMER_RIGHT_49_TM=59.997
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=620
=
SEQUENCE_ID=MAGIC.338_3_2627870_2627920_ws-0_po-0_256271_456791_TRUE
SEQUENCE_TARGET=950,150
SEQUENCE_TEMPLATE=AATGCATCATTTTGATCTCCAAGCAAGTTTTCTCCTTACAAGAGATTAGAGAAAACCAAATTATCTCTGAGGCTTTGAAGAATCAAAACTGAATCAAGAGGANGTTTCCTGTTTAACAAAGATCACAAGAACAAATTTCNAAAAAGAAACTGTAACGAAGAACAACAAAGCAACAATCCTCGCAATATAACAAATAGTTCGTACAGAATCGTTCCTATTCNCCTTTCNATGGACAAAAGCGTTTNAAAAACGCTAATTTCTAGCAATTCGTTTTACCAGGTTANNNNNNNNNNNNNCAATTTCNGAAATTAACGATTTGATCTACNGTTCTACNCAATTTACCCCATCCAGATTAATGCTAGAANAAAANGAACGGGATCGCAGAACTTACCAGCACAGTAAAAATCAGAGCACGAAGAATGTTGGATCGGACGAACATGAGCAAAATTGGAGGCNAATGAAGAAGATTTTCAGGAGATATGATGAAAAAATTTGAAACCCNNAAAAAAAAGAAAAGGAAACCTNGTGCGACGACCGGATCCCTTCTCGCCTTAGGAGACTTTGGGATAAATCATGGAAGCCAAATTTTATCAAAGGAATATCCACTGACATTTATTTATTTTTACATTNAAAAAAANNNAAAAAAACTCACATNTAAANTATTTGTGATAAAGACCCTCAAGTTTGCTAATCTACCCAAAAGAGTCTGAATTATGTAAAATTATTATTGACGTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGATAATATTACATCGTTTACATATGAGTTCATCGTGAGTTCACGTCTGCTGACTTATTTTTGTAATTATTTATCTTCTTAACAAAAAAAACAGGTTTATCATTACATGGATATAGACTTATAGTCGACAATATTTGACACTCAAGCTGAAAGAACATGCTACAAAACATGTTGTGTGGACATTTATCNTATCTTAGTTGATATTATNAGANTTTTNAAGATGTTTGTTGGCTCTTGTGATTAAGAATCCAGTATACTGAACTAAGNACACAACATCAACAATGCTTATAATAAGAAAACAATAAAGATAAATACATNAAGCATACCACCAAATCGATCGATCAATAAATTCCATCTATTANAAGCTATAGCGATGGTTATACCTACGATATATGTGGCACCGATCCTATGTCCCAGACAACTTGATAAATGAGTTACNGTACTCTTTTGTATAATACTAGATTTTTGTGGTACAGAAAACTACACATCAACCTTGGCTCATGAACATGTATATGAATGAAAAGCAAGAGAGGCTACAATAAGAACATACCAAAACAAACACAGCGATGCGCATTAAGTGATTAAATAAACTTTAGAGATCTCTCCCAAAAGTATATACTTTAACTTTGTCCTTTGCTANTTGTTTGTTCATCTTTTATTTGGTGGGTTTTGATATGACATCGACTTCATTTCTCAGTTCNCAATCCATTTTATTTTAACTAAAAATGGGTNNAAGTCTTGCTTGAACAAGTTTGGACAAACTTCAACCAAACTANGTATAGTGTTTTATTGTATTTATGGTTTAACGTGTTGTTGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.389723
PRIMER_LEFT_0_PENALTY=0.277599
PRIMER_RIGHT_0_PENALTY=0.112124
PRIMER_LEFT_0_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_0_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_0=562,20
PRIMER_RIGHT_0=1286,20
PRIMER_LEFT_0_TM=59.722
PRIMER_RIGHT_0_TM=60.112
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=725
PRIMER_PAIR_1_PENALTY=0.418274
PRIMER_LEFT_1_PENALTY=0.306150
PRIMER_RIGHT_1_PENALTY=0.112124
PRIMER_LEFT_1_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_1_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_1=374,20
PRIMER_RIGHT_1=1286,20
PRIMER_LEFT_1_TM=59.694
PRIMER_RIGHT_1_TM=60.112
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=913
PRIMER_PAIR_2_PENALTY=0.426943
PRIMER_LEFT_2_PENALTY=0.277599
PRIMER_RIGHT_2_PENALTY=0.149344
PRIMER_LEFT_2_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_2_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_2=562,20
PRIMER_RIGHT_2=1352,20
PRIMER_LEFT_2_TM=59.722
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=791
PRIMER_PAIR_3_PENALTY=0.447803
PRIMER_LEFT_3_PENALTY=0.335679
PRIMER_RIGHT_3_PENALTY=0.112124
PRIMER_LEFT_3_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_3_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_3=432,20
PRIMER_RIGHT_3=1286,20
PRIMER_LEFT_3_TM=59.664
PRIMER_RIGHT_3_TM=60.112
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=855
PRIMER_PAIR_4_PENALTY=0.454113
PRIMER_LEFT_4_PENALTY=0.341988
PRIMER_RIGHT_4_PENALTY=0.112124
PRIMER_LEFT_4_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_4_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_4=379,20
PRIMER_RIGHT_4=1286,20
PRIMER_LEFT_4_TM=59.658
PRIMER_RIGHT_4_TM=60.112
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=908
PRIMER_PAIR_5_PENALTY=0.485022
PRIMER_LEFT_5_PENALTY=0.335679
PRIMER_RIGHT_5_PENALTY=0.149344
PRIMER_LEFT_5_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_5_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_5=432,20
PRIMER_RIGHT_5=1352,20
PRIMER_LEFT_5_TM=59.664
PRIMER_RIGHT_5_TM=60.149
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=921
PRIMER_PAIR_6_PENALTY=0.491332
PRIMER_LEFT_6_PENALTY=0.341988
PRIMER_RIGHT_6_PENALTY=0.149344
PRIMER_LEFT_6_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_6_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_6=379,20
PRIMER_RIGHT_6=1352,20
PRIMER_LEFT_6_TM=59.658
PRIMER_RIGHT_6_TM=60.149
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=974
PRIMER_PAIR_7_PENALTY=0.579537
PRIMER_LEFT_7_PENALTY=0.277599
PRIMER_RIGHT_7_PENALTY=0.301938
PRIMER_LEFT_7_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_7_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_7=562,20
PRIMER_RIGHT_7=1353,20
PRIMER_LEFT_7_TM=59.722
PRIMER_RIGHT_7_TM=60.302
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=792
PRIMER_PAIR_8_PENALTY=0.637616
PRIMER_LEFT_8_PENALTY=0.335679
PRIMER_RIGHT_8_PENALTY=0.301938
PRIMER_LEFT_8_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_8_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_8=432,20
PRIMER_RIGHT_8=1353,20
PRIMER_LEFT_8_TM=59.664
PRIMER_RIGHT_8_TM=60.302
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=922
PRIMER_PAIR_9_PENALTY=0.913753
PRIMER_LEFT_9_PENALTY=0.801629
PRIMER_RIGHT_9_PENALTY=0.112124
PRIMER_LEFT_9_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_9_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_9=371,20
PRIMER_RIGHT_9=1286,20
PRIMER_LEFT_9_TM=59.198
PRIMER_RIGHT_9_TM=60.112
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=916
PRIMER_PAIR_10_PENALTY=0.950973
PRIMER_LEFT_10_PENALTY=0.801629
PRIMER_RIGHT_10_PENALTY=0.149344
PRIMER_LEFT_10_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_10_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_10=371,20
PRIMER_RIGHT_10=1352,20
PRIMER_LEFT_10_TM=59.198
PRIMER_RIGHT_10_TM=60.149
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.7000
PRIMER_RIGHT_10_END_STABILITY=8.8000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=982
PRIMER_PAIR_11_PENALTY=1.084379
PRIMER_LEFT_11_PENALTY=0.972254
PRIMER_RIGHT_11_PENALTY=0.112124
PRIMER_LEFT_11_SEQUENCE=GATCGCAGAACTTACCAGCA
PRIMER_RIGHT_11_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_11=376,20
PRIMER_RIGHT_11=1286,20
PRIMER_LEFT_11_TM=59.028
PRIMER_RIGHT_11_TM=60.112
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=911
PRIMER_PAIR_12_PENALTY=1.159132
PRIMER_LEFT_12_PENALTY=0.277599
PRIMER_RIGHT_12_PENALTY=0.881533
PRIMER_LEFT_12_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_12_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_12=562,20
PRIMER_RIGHT_12=1201,20
PRIMER_LEFT_12_TM=59.722
PRIMER_RIGHT_12_TM=60.882
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=9.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=640
PRIMER_PAIR_13_PENALTY=1.159132
PRIMER_LEFT_13_PENALTY=0.277599
PRIMER_RIGHT_13_PENALTY=0.881533
PRIMER_LEFT_13_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_13_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_13=562,20
PRIMER_RIGHT_13=1203,20
PRIMER_LEFT_13_TM=59.722
PRIMER_RIGHT_13_TM=60.882
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=9.8000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=642
PRIMER_PAIR_14_PENALTY=1.187683
PRIMER_LEFT_14_PENALTY=0.306150
PRIMER_RIGHT_14_PENALTY=0.881533
PRIMER_LEFT_14_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_14_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_14=374,20
PRIMER_RIGHT_14=1201,20
PRIMER_LEFT_14_TM=59.694
PRIMER_RIGHT_14_TM=60.882
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=828
PRIMER_PAIR_15_PENALTY=1.187683
PRIMER_LEFT_15_PENALTY=0.306150
PRIMER_RIGHT_15_PENALTY=0.881533
PRIMER_LEFT_15_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_15_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_15=374,20
PRIMER_RIGHT_15=1203,20
PRIMER_LEFT_15_TM=59.694
PRIMER_RIGHT_15_TM=60.882
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=9.8000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=830
PRIMER_PAIR_16_PENALTY=1.215627
PRIMER_LEFT_16_PENALTY=1.103503
PRIMER_RIGHT_16_PENALTY=0.112124
PRIMER_LEFT_16_SEQUENCE=GATCGCAGAACTTACCAGCAC
PRIMER_RIGHT_16_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_16=376,21
PRIMER_RIGHT_16=1286,20
PRIMER_LEFT_16_TM=59.896
PRIMER_RIGHT_16_TM=60.112
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=911
PRIMER_PAIR_17_PENALTY=1.217212
PRIMER_LEFT_17_PENALTY=0.335679
PRIMER_RIGHT_17_PENALTY=0.881533
PRIMER_LEFT_17_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_17_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_17=432,20
PRIMER_RIGHT_17=1201,20
PRIMER_LEFT_17_TM=59.664
PRIMER_RIGHT_17_TM=60.882
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=770
PRIMER_PAIR_18_PENALTY=1.217212
PRIMER_LEFT_18_PENALTY=0.335679
PRIMER_RIGHT_18_PENALTY=0.881533
PRIMER_LEFT_18_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_18_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_18=432,20
PRIMER_RIGHT_18=1203,20
PRIMER_LEFT_18_TM=59.664
PRIMER_RIGHT_18_TM=60.882
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=9.8000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=772
PRIMER_PAIR_19_PENALTY=1.223522
PRIMER_LEFT_19_PENALTY=0.341988
PRIMER_RIGHT_19_PENALTY=0.881533
PRIMER_LEFT_19_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_19_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_19=379,20
PRIMER_RIGHT_19=1201,20
PRIMER_LEFT_19_TM=59.658
PRIMER_RIGHT_19_TM=60.882
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=823
PRIMER_PAIR_20_PENALTY=1.223522
PRIMER_LEFT_20_PENALTY=0.341988
PRIMER_RIGHT_20_PENALTY=0.881533
PRIMER_LEFT_20_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_20_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_20=379,20
PRIMER_RIGHT_20=1203,20
PRIMER_LEFT_20_TM=59.658
PRIMER_RIGHT_20_TM=60.882
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=9.8000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=825
PRIMER_PAIR_21_PENALTY=1.252847
PRIMER_LEFT_21_PENALTY=1.103503
PRIMER_RIGHT_21_PENALTY=0.149344
PRIMER_LEFT_21_SEQUENCE=GATCGCAGAACTTACCAGCAC
PRIMER_RIGHT_21_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_21=376,21
PRIMER_RIGHT_21=1352,20
PRIMER_LEFT_21_TM=59.896
PRIMER_RIGHT_21_TM=60.149
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=977
PRIMER_PAIR_22_PENALTY=1.477975
PRIMER_LEFT_22_PENALTY=0.596442
PRIMER_RIGHT_22_PENALTY=0.881533
PRIMER_LEFT_22_SEQUENCE=TCGCAGAACTTACCAGCACA
PRIMER_RIGHT_22_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_22=378,20
PRIMER_RIGHT_22=1201,20
PRIMER_LEFT_22_TM=60.596
PRIMER_RIGHT_22_TM=60.882
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=9.9000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=824
PRIMER_PAIR_23_PENALTY=1.477975
PRIMER_LEFT_23_PENALTY=0.596442
PRIMER_RIGHT_23_PENALTY=0.881533
PRIMER_LEFT_23_SEQUENCE=TCGCAGAACTTACCAGCACA
PRIMER_RIGHT_23_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_23=378,20
PRIMER_RIGHT_23=1203,20
PRIMER_LEFT_23_TM=60.596
PRIMER_RIGHT_23_TM=60.882
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=9.8000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=826
PRIMER_PAIR_24_PENALTY=1.513358
PRIMER_LEFT_24_PENALTY=1.401234
PRIMER_RIGHT_24_PENALTY=0.112124
PRIMER_LEFT_24_SEQUENCE=ACGGGATCGCAGAACTTACC
PRIMER_RIGHT_24_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_24=372,20
PRIMER_RIGHT_24=1286,20
PRIMER_LEFT_24_TM=61.401
PRIMER_RIGHT_24_TM=60.112
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.2000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=915
PRIMER_PAIR_25_PENALTY=1.548126
PRIMER_LEFT_25_PENALTY=1.436002
PRIMER_RIGHT_25_PENALTY=0.112124
PRIMER_LEFT_25_SEQUENCE=CACGAAGAATGTTGGATCGG
PRIMER_RIGHT_25_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_25=411,20
PRIMER_RIGHT_25=1286,20
PRIMER_LEFT_25_TM=61.436
PRIMER_RIGHT_25_TM=60.112
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.8000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=876
PRIMER_PAIR_26_PENALTY=1.550577
PRIMER_LEFT_26_PENALTY=1.401234
PRIMER_RIGHT_26_PENALTY=0.149344
PRIMER_LEFT_26_SEQUENCE=ACGGGATCGCAGAACTTACC
PRIMER_RIGHT_26_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_26=372,20
PRIMER_RIGHT_26=1352,20
PRIMER_LEFT_26_TM=61.401
PRIMER_RIGHT_26_TM=60.149
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.2000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=981
PRIMER_PAIR_27_PENALTY=1.552062
PRIMER_LEFT_27_PENALTY=1.439937
PRIMER_RIGHT_27_PENALTY=0.112124
PRIMER_LEFT_27_SEQUENCE=GAACATGAGCAAAATTGGAGG
PRIMER_RIGHT_27_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_27=433,21
PRIMER_RIGHT_27=1286,20
PRIMER_LEFT_27_TM=59.560
PRIMER_RIGHT_27_TM=60.112
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=854
PRIMER_PAIR_28_PENALTY=1.585346
PRIMER_LEFT_28_PENALTY=1.436002
PRIMER_RIGHT_28_PENALTY=0.149344
PRIMER_LEFT_28_SEQUENCE=CACGAAGAATGTTGGATCGG
PRIMER_RIGHT_28_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_28=411,20
PRIMER_RIGHT_28=1352,20
PRIMER_LEFT_28_TM=61.436
PRIMER_RIGHT_28_TM=60.149
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.8000
PRIMER_RIGHT_28_END_STABILITY=8.8000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=942
PRIMER_PAIR_29_PENALTY=1.589281
PRIMER_LEFT_29_PENALTY=1.439937
PRIMER_RIGHT_29_PENALTY=0.149344
PRIMER_LEFT_29_SEQUENCE=GAACATGAGCAAAATTGGAGG
PRIMER_RIGHT_29_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_29=433,21
PRIMER_RIGHT_29=1352,20
PRIMER_LEFT_29_TM=59.560
PRIMER_RIGHT_29_TM=60.149
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=920
PRIMER_PAIR_30_PENALTY=1.669338
PRIMER_LEFT_30_PENALTY=1.557214
PRIMER_RIGHT_30_PENALTY=0.112124
PRIMER_LEFT_30_SEQUENCE=AGAGCACGAAGAATGTTGGA
PRIMER_RIGHT_30_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_30=407,20
PRIMER_RIGHT_30=1286,20
PRIMER_LEFT_30_TM=58.443
PRIMER_RIGHT_30_TM=60.112
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=880
PRIMER_PAIR_31_PENALTY=1.683162
PRIMER_LEFT_31_PENALTY=0.801629
PRIMER_RIGHT_31_PENALTY=0.881533
PRIMER_LEFT_31_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_31_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_31=371,20
PRIMER_RIGHT_31=1201,20
PRIMER_LEFT_31_TM=59.198
PRIMER_RIGHT_31_TM=60.882
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.7000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=831
PRIMER_PAIR_32_PENALTY=1.683162
PRIMER_LEFT_32_PENALTY=0.801629
PRIMER_RIGHT_32_PENALTY=0.881533
PRIMER_LEFT_32_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_32_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_32=371,20
PRIMER_RIGHT_32=1203,20
PRIMER_LEFT_32_TM=59.198
PRIMER_RIGHT_32_TM=60.882
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=5.7000
PRIMER_RIGHT_32_END_STABILITY=9.8000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=833
PRIMER_PAIR_33_PENALTY=1.700056
PRIMER_LEFT_33_PENALTY=1.587932
PRIMER_RIGHT_33_PENALTY=0.112124
PRIMER_LEFT_33_SEQUENCE=ATCGGACGAACATGAGCAAA
PRIMER_RIGHT_33_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_33=426,20
PRIMER_RIGHT_33=1286,20
PRIMER_LEFT_33_TM=61.588
PRIMER_RIGHT_33_TM=60.112
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.8000
PRIMER_RIGHT_33_END_STABILITY=6.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=861
PRIMER_PAIR_34_PENALTY=1.703171
PRIMER_LEFT_34_PENALTY=1.401234
PRIMER_RIGHT_34_PENALTY=0.301938
PRIMER_LEFT_34_SEQUENCE=ACGGGATCGCAGAACTTACC
PRIMER_RIGHT_34_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_34=372,20
PRIMER_RIGHT_34=1353,20
PRIMER_LEFT_34_TM=61.401
PRIMER_RIGHT_34_TM=60.302
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.2000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=982
PRIMER_PAIR_35_PENALTY=1.706557
PRIMER_LEFT_35_PENALTY=1.557214
PRIMER_RIGHT_35_PENALTY=0.149344
PRIMER_LEFT_35_SEQUENCE=AGAGCACGAAGAATGTTGGA
PRIMER_RIGHT_35_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_35=407,20
PRIMER_RIGHT_35=1352,20
PRIMER_LEFT_35_TM=58.443
PRIMER_RIGHT_35_TM=60.149
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=8.8000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=946
PRIMER_PAIR_36_PENALTY=1.723117
PRIMER_LEFT_36_PENALTY=0.306150
PRIMER_RIGHT_36_PENALTY=1.416967
PRIMER_LEFT_36_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_36_SEQUENCE=GCCTCTCTTGCTTTTCATTCA
PRIMER_LEFT_36=374,20
PRIMER_RIGHT_36=1317,21
PRIMER_LEFT_36_TM=59.694
PRIMER_RIGHT_36_TM=59.583
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=944
PRIMER_PAIR_37_PENALTY=1.732426
PRIMER_LEFT_37_PENALTY=1.620302
PRIMER_RIGHT_37_PENALTY=0.112124
PRIMER_LEFT_37_SEQUENCE=CGCCTTAGGAGACTTTGGGA
PRIMER_RIGHT_37_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_37=547,20
PRIMER_RIGHT_37=1286,20
PRIMER_LEFT_37_TM=61.620
PRIMER_RIGHT_37_TM=60.112
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=740
PRIMER_PAIR_38_PENALTY=1.734786
PRIMER_LEFT_38_PENALTY=1.622662
PRIMER_RIGHT_38_PENALTY=0.112124
PRIMER_LEFT_38_SEQUENCE=CGAACATGAGCAAAATTGGAG
PRIMER_RIGHT_38_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_38=432,21
PRIMER_RIGHT_38=1286,20
PRIMER_LEFT_38_TM=60.623
PRIMER_RIGHT_38_TM=60.112
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=855
PRIMER_PAIR_39_PENALTY=1.737276
PRIMER_LEFT_39_PENALTY=1.587932
PRIMER_RIGHT_39_PENALTY=0.149344
PRIMER_LEFT_39_SEQUENCE=ATCGGACGAACATGAGCAAA
PRIMER_RIGHT_39_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_39=426,20
PRIMER_RIGHT_39=1352,20
PRIMER_LEFT_39_TM=61.588
PRIMER_RIGHT_39_TM=60.149
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.8000
PRIMER_RIGHT_39_END_STABILITY=8.8000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=927
PRIMER_PAIR_40_PENALTY=1.737939
PRIMER_LEFT_40_PENALTY=1.436002
PRIMER_RIGHT_40_PENALTY=0.301938
PRIMER_LEFT_40_SEQUENCE=CACGAAGAATGTTGGATCGG
PRIMER_RIGHT_40_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_40=411,20
PRIMER_RIGHT_40=1353,20
PRIMER_LEFT_40_TM=61.436
PRIMER_RIGHT_40_TM=60.302
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.8000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=943
PRIMER_PAIR_41_PENALTY=1.741875
PRIMER_LEFT_41_PENALTY=1.439937
PRIMER_RIGHT_41_PENALTY=0.301938
PRIMER_LEFT_41_SEQUENCE=GAACATGAGCAAAATTGGAGG
PRIMER_RIGHT_41_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_41=433,21
PRIMER_RIGHT_41=1353,20
PRIMER_LEFT_41_TM=59.560
PRIMER_RIGHT_41_TM=60.302
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=921
PRIMER_PAIR_42_PENALTY=1.752646
PRIMER_LEFT_42_PENALTY=0.335679
PRIMER_RIGHT_42_PENALTY=1.416967
PRIMER_LEFT_42_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_42_SEQUENCE=GCCTCTCTTGCTTTTCATTCA
PRIMER_LEFT_42=432,20
PRIMER_RIGHT_42=1317,21
PRIMER_LEFT_42_TM=59.664
PRIMER_RIGHT_42_TM=59.583
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=886
PRIMER_PAIR_43_PENALTY=1.758955
PRIMER_LEFT_43_PENALTY=0.341988
PRIMER_RIGHT_43_PENALTY=1.416967
PRIMER_LEFT_43_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_43_SEQUENCE=GCCTCTCTTGCTTTTCATTCA
PRIMER_LEFT_43=379,20
PRIMER_RIGHT_43=1317,21
PRIMER_LEFT_43_TM=59.658
PRIMER_RIGHT_43_TM=59.583
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=939
PRIMER_PAIR_44_PENALTY=1.769645
PRIMER_LEFT_44_PENALTY=1.620302
PRIMER_RIGHT_44_PENALTY=0.149344
PRIMER_LEFT_44_SEQUENCE=CGCCTTAGGAGACTTTGGGA
PRIMER_RIGHT_44_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_44=547,20
PRIMER_RIGHT_44=1352,20
PRIMER_LEFT_44_TM=61.620
PRIMER_RIGHT_44_TM=60.149
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.7000
PRIMER_RIGHT_44_END_STABILITY=8.8000
PRIMER_PAIR_44_COMPL_ANY=2.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=806
PRIMER_PAIR_45_PENALTY=1.772006
PRIMER_LEFT_45_PENALTY=1.622662
PRIMER_RIGHT_45_PENALTY=0.149344
PRIMER_LEFT_45_SEQUENCE=CGAACATGAGCAAAATTGGAG
PRIMER_RIGHT_45_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_45=432,21
PRIMER_RIGHT_45=1352,20
PRIMER_LEFT_45_TM=60.623
PRIMER_RIGHT_45_TM=60.149
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=921
PRIMER_PAIR_46_PENALTY=1.853788
PRIMER_LEFT_46_PENALTY=0.972254
PRIMER_RIGHT_46_PENALTY=0.881533
PRIMER_LEFT_46_SEQUENCE=GATCGCAGAACTTACCAGCA
PRIMER_RIGHT_46_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_46=376,20
PRIMER_RIGHT_46=1201,20
PRIMER_LEFT_46_TM=59.028
PRIMER_RIGHT_46_TM=60.882
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=826
PRIMER_PAIR_47_PENALTY=1.853788
PRIMER_LEFT_47_PENALTY=0.972254
PRIMER_RIGHT_47_PENALTY=0.881533
PRIMER_LEFT_47_SEQUENCE=GATCGCAGAACTTACCAGCA
PRIMER_RIGHT_47_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_47=376,20
PRIMER_RIGHT_47=1203,20
PRIMER_LEFT_47_TM=59.028
PRIMER_RIGHT_47_TM=60.882
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=9.8000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=828
PRIMER_PAIR_48_PENALTY=1.859151
PRIMER_LEFT_48_PENALTY=1.557214
PRIMER_RIGHT_48_PENALTY=0.301938
PRIMER_LEFT_48_SEQUENCE=AGAGCACGAAGAATGTTGGA
PRIMER_RIGHT_48_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_48=407,20
PRIMER_RIGHT_48=1353,20
PRIMER_LEFT_48_TM=58.443
PRIMER_RIGHT_48_TM=60.302
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=947
PRIMER_PAIR_49_PENALTY=1.922239
PRIMER_LEFT_49_PENALTY=1.620302
PRIMER_RIGHT_49_PENALTY=0.301938
PRIMER_LEFT_49_SEQUENCE=CGCCTTAGGAGACTTTGGGA
PRIMER_RIGHT_49_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_49=547,20
PRIMER_RIGHT_49=1353,20
PRIMER_LEFT_49_TM=61.620
PRIMER_RIGHT_49_TM=60.302
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=807
=
SEQUENCE_ID=MAGIC.329_3_9015842_9015861_ler-0_wil-2_2895_1258_TRUE
SEQUENCE_TARGET=950,119
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_15879801_15879828_zu-0_no-0_227029_536981_TRUE
SEQUENCE_TARGET=950,127
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_1460008_1460271_rsch-4_po-0_142912_79772_TRUE
SEQUENCE_TARGET=950,363
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_13161245_13161279_hi-0_ws-0_9264_2719_TRUE
SEQUENCE_TARGET=950,134
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAAACGAATTCAGCTATTAATAAAATTTGTTTTANTNATTTNAAGANNAGTTNGACTANACNAACAAACNNAGGAAANGAGNTNGNCNGTNCNAACATNCAAAACANGAAGCANNGNTACATTTATNNAAAGGCNAAAACANTAAAAANNGTCNAANTCTTNAACACANTTNNAACTAGGAAATCNTTCTCGTCTTGNAAAANACTTCGACTCCTTNGTTGGCAAGATCAAGGATGGGCATCATANANTCCGNGATGNGAGCAAATTCNGCGATTCCANNNNNNNNNNNNNNNNNNNNNNNNNAGTTGANGGTGNGTAGCTCCCTTGATATCANGGTGACTCTNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCNTTTNTGATGAACGCTTTTGNNGTTGAGNCGNGANAGCTTTGATNAANGCCATNGCAATTTNTNGNTCTCTGCTTGAAAGATCNTTGATTAATGCACATGGNGCTCNNATCTGNGATCTGAAGTTGNNAANTGGNNAGCGTCCTNTNTCNGNNACNTTCNGGATATNCTCATCAATCANNNAACCCTGGNAAAGTCTGNNNNNNNNNNNNNGCATNGANGGTTAAAAGCCANNANTNCACAAAAGGAAAAACGAANGNTAGNTGAANAANAANNAAAACANAGAGAANCAATTTATAGNAGGATTCNTAANTTTCAAANANGCGATTCCATTCANNGCCNGAGTGATTNNCNGAAGCATTNACGTCNCTCCNATCNNCNTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_6890095_6890486_po-0_can-0_27750_17216_TRUE
SEQUENCE_TARGET=950,491
SEQUENCE_TEMPLATE=TTTAAATCCAGTGATTATAAAGCGTATTAGAGATTATAAATTAAGGTTACAATTGAANTTTTTTNAGATTTAATCGGAACTAGACCTATGCCACTAACGATGTTTAGTTAATGTTAACTGAGTGCCGTCTTGGCATTGTTTGAAACAAATTTGGNATTCAAATCTTCAATGAGATAGTATCATACTACTAAAAANTTGAAACTTAGGTCNTTTTTGGCATTGTTTCNTTATATGGTNAAANATTAAATACTTACATTTCTTCTTCTTGATATATCGTATGGTNGAGGTTATATCACCTCATTTATGTTTTGTATTTAAGGATCGGTGGTGGAGATCTATAACATCATTCATGTTTGGTTCGTGTCTTTGTAGGGAGCTGTTGGGAGTTCTCGACACGATGGTNACAAGGAGAATTTAACACTTTGACTGAGCAAGACTTGATTGATTCATTCTAATAAAGAAAATTATGGTTGTGGAGGAGGCAAAATCGAGACNGCGGATGAGTTCATATATANGTAGCAATGGTGGTCTTGGTATNNNCAAGTATTATCCATATAAGGCTGTTACTGGAGCTTGCGACGGCCCCTCAAGATTGGTAGTTANNNNNTTACAATCATAGAGATTAGGTATCATGTCTAACAAAAAACAAAAGTAATTCTTGAAAANATAAAAATAAATNGTAAGTAGCATATAATANCAAGAATGTTATGAGTGATAGTATGAGAACCAGCCTGTGACTGTTGTCATTGATTCAAGCGATCGGAAATTTCACTTTATTTATCAATNATATATATAACATTCATNTTTTTCAAAATAACTATATCTTNATAGAAGTCTCACGANTCGATACTTCGATCGACCACAACCAGACGTAGTCTCGTGTGANTGTGTGACTAACTTTAAAAACAACAATAATNAAATCAAAACACGAAAATCTAATTCTTACGTCCTTGTTTTTTAAATTCTCTTACGTCAATTCTTACCTTTGCGACCAAAAGAANATGTTAAATTTAAACCTACTAATTTCCGATTTAAATTTCTTAATCTTGTTACTATGAACCAAAAATTACACAATCTTTTCCCNCGGAACAAAGATTATTTATTAGTATCCATATACATACAAATATACAATACACTCAAGCCACTCATATTTCNTACTTGATCAAGATATNTTCCCCCGNTAAAATCCAATTTAGAATTGTACAACATATATATTGGAAGTTCCGTTGTGTCATACCGTCACACTAATAATACAGCATACCAATAATAGAGCGCAAACGTTTCACATTGAATAGTTCACCACATATCAANTTGAGTAAATNATTATNCNNAGAGTTGTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGTGAAGATAAACGAAACAAAACTTGAGGGAAAAAAAACAGTCACGTGAGTCATGNGTTATAAAGCATNGATAATNTTGGAGATCTANNTAATCTAATTGAATGCCACATCAAATTAAGCCAATCAGTGGAGAAGNTCAATAATAANAGTGAATTGGATCTGCCACTACACTTTTAGCATGAATNNNNNNNNAAGNGATTGGTATTGGTAATGGTATCGTTNTCGGAGATTGTCGTACCAATTAAATAACATGNAANTATTGGATATATATTATATTGTGTCGGCCTAGTCATNTTTGATGTGTAAGTGCGAGTGATTTGAGTTAGATATNGGGTATTTTANGAGGAAAATATTGNANNNNNNNNNNNNNNNNNNNGNTTGTAGTTTGAGNATCACAAGTTAATTATATTNAGGTTCTGCNTNAATTTGACTCAAAAGATTANGTTATCAAAAGAAACCTCGTATANTATGATCCATATTANTTTTCNNTNTTTTATATAGAAAAGTTGAAGATTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNATGAATGTGACTTTGAGTTGAAAGTTTGAAAANGAAGAGACTATAACATTTGAAGGAATCTATGNANTANNGGNGTGGAANCAATAGTGGAGGCCCTCTTNATAAACCGAGTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.097043
PRIMER_LEFT_0_PENALTY=0.255244
PRIMER_RIGHT_0_PENALTY=2.841800
PRIMER_LEFT_0_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_0_SEQUENCE=AGTGGCAGATCCAATTCACT
PRIMER_LEFT_0=855,20
PRIMER_RIGHT_0=1724,20
PRIMER_LEFT_0_TM=59.745
PRIMER_RIGHT_0_TM=57.158
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.4000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=870
PRIMER_PAIR_1_PENALTY=3.388531
PRIMER_LEFT_1_PENALTY=0.690085
PRIMER_RIGHT_1_PENALTY=2.698446
PRIMER_LEFT_1_SEQUENCE=GAACCAGCCTGTGACTGTTG
PRIMER_RIGHT_1_SEQUENCE=TCCCTCAAGTTTTGTTTCGT
PRIMER_LEFT_1=723,20
PRIMER_RIGHT_1=1588,20
PRIMER_LEFT_1_TM=59.310
PRIMER_RIGHT_1_TM=57.302
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=866
PRIMER_PAIR_2_PENALTY=3.398025
PRIMER_LEFT_2_PENALTY=0.255244
PRIMER_RIGHT_2_PENALTY=3.142781
PRIMER_LEFT_2_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_2_SEQUENCE=CCCTCAAGTTTTGTTTCGTT
PRIMER_LEFT_2=855,20
PRIMER_RIGHT_2=1587,20
PRIMER_LEFT_2_TM=59.745
PRIMER_RIGHT_2_TM=56.857
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=733
PRIMER_PAIR_3_PENALTY=3.487956
PRIMER_LEFT_3_PENALTY=0.255244
PRIMER_RIGHT_3_PENALTY=3.232712
PRIMER_LEFT_3_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_3_SEQUENCE=AAAAGTGTAGTGGCAGATCCAA
PRIMER_LEFT_3=855,20
PRIMER_RIGHT_3=1732,22
PRIMER_LEFT_3_TM=59.745
PRIMER_RIGHT_3_TM=58.767
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=878
PRIMER_PAIR_4_PENALTY=3.491662
PRIMER_LEFT_4_PENALTY=0.255244
PRIMER_RIGHT_4_PENALTY=3.236418
PRIMER_LEFT_4_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_4_SEQUENCE=CTCCACTGATTGGCTTAATTTG
PRIMER_LEFT_4=855,20
PRIMER_RIGHT_4=1689,22
PRIMER_LEFT_4_TM=59.745
PRIMER_RIGHT_4_TM=58.764
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=835
PRIMER_PAIR_5_PENALTY=3.777782
PRIMER_LEFT_5_PENALTY=0.255244
PRIMER_RIGHT_5_PENALTY=3.522538
PRIMER_LEFT_5_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_5_SEQUENCE=CATGCTAAAAGTGTAGTGGCAGA
PRIMER_LEFT_5=855,20
PRIMER_RIGHT_5=1738,23
PRIMER_LEFT_5_TM=59.745
PRIMER_RIGHT_5_TM=59.477
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.4000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=884
PRIMER_PAIR_6_PENALTY=3.777782
PRIMER_LEFT_6_PENALTY=0.255244
PRIMER_RIGHT_6_PENALTY=3.522538
PRIMER_LEFT_6_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_6_SEQUENCE=TCATGCTAAAAGTGTAGTGGCAG
PRIMER_LEFT_6=855,20
PRIMER_RIGHT_6=1739,23
PRIMER_LEFT_6_TM=59.745
PRIMER_RIGHT_6_TM=59.477
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.4000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=885
PRIMER_PAIR_7_PENALTY=3.832866
PRIMER_LEFT_7_PENALTY=0.690085
PRIMER_RIGHT_7_PENALTY=3.142781
PRIMER_LEFT_7_SEQUENCE=GAACCAGCCTGTGACTGTTG
PRIMER_RIGHT_7_SEQUENCE=CCCTCAAGTTTTGTTTCGTT
PRIMER_LEFT_7=723,20
PRIMER_RIGHT_7=1587,20
PRIMER_LEFT_7_TM=59.310
PRIMER_RIGHT_7_TM=56.857
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=865
PRIMER_PAIR_8_PENALTY=3.884890
PRIMER_LEFT_8_PENALTY=1.043090
PRIMER_RIGHT_8_PENALTY=2.841800
PRIMER_LEFT_8_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_8_SEQUENCE=AGTGGCAGATCCAATTCACT
PRIMER_LEFT_8=854,21
PRIMER_RIGHT_8=1724,20
PRIMER_LEFT_8_TM=60.043
PRIMER_RIGHT_8_TM=57.158
PRIMER_LEFT_8_GC_PERCENT=52.381
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.4000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=871
PRIMER_PAIR_9_PENALTY=3.910964
PRIMER_LEFT_9_PENALTY=1.212518
PRIMER_RIGHT_9_PENALTY=2.698446
PRIMER_LEFT_9_SEQUENCE=GAACCAGCCTGTGACTGTTGT
PRIMER_RIGHT_9_SEQUENCE=TCCCTCAAGTTTTGTTTCGT
PRIMER_LEFT_9=723,21
PRIMER_RIGHT_9=1588,20
PRIMER_LEFT_9_TM=60.213
PRIMER_RIGHT_9_TM=57.302
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=866
PRIMER_PAIR_10_PENALTY=3.911242
PRIMER_LEFT_10_PENALTY=0.255244
PRIMER_RIGHT_10_PENALTY=3.655999
PRIMER_LEFT_10_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_10_SEQUENCE=AAAGTGTAGTGGCAGATCCAA
PRIMER_LEFT_10=855,20
PRIMER_RIGHT_10=1731,21
PRIMER_LEFT_10_TM=59.745
PRIMER_RIGHT_10_TM=57.344
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.4000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=877
PRIMER_PAIR_11_PENALTY=3.926503
PRIMER_LEFT_11_PENALTY=0.690085
PRIMER_RIGHT_11_PENALTY=3.236418
PRIMER_LEFT_11_SEQUENCE=GAACCAGCCTGTGACTGTTG
PRIMER_RIGHT_11_SEQUENCE=CTCCACTGATTGGCTTAATTTG
PRIMER_LEFT_11=723,20
PRIMER_RIGHT_11=1689,22
PRIMER_LEFT_11_TM=59.310
PRIMER_RIGHT_11_TM=58.764
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=967
PRIMER_PAIR_12_PENALTY=3.973867
PRIMER_LEFT_12_PENALTY=0.255244
PRIMER_RIGHT_12_PENALTY=3.718624
PRIMER_LEFT_12_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_12_SEQUENCE=TAGTGGCAGATCCAATTCACT
PRIMER_LEFT_12=855,20
PRIMER_RIGHT_12=1725,21
PRIMER_LEFT_12_TM=59.745
PRIMER_RIGHT_12_TM=57.281
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.4000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=871
PRIMER_PAIR_13_PENALTY=4.048613
PRIMER_LEFT_13_PENALTY=1.350166
PRIMER_RIGHT_13_PENALTY=2.698446
PRIMER_LEFT_13_SEQUENCE=AGAACCAGCCTGTGACTGTTG
PRIMER_RIGHT_13_SEQUENCE=TCCCTCAAGTTTTGTTTCGT
PRIMER_LEFT_13=722,21
PRIMER_RIGHT_13=1588,20
PRIMER_LEFT_13_TM=60.350
PRIMER_RIGHT_13_TM=57.302
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=867
PRIMER_PAIR_14_PENALTY=4.054707
PRIMER_LEFT_14_PENALTY=0.255244
PRIMER_RIGHT_14_PENALTY=3.799463
PRIMER_LEFT_14_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_14_SEQUENCE=GTAGTGGCAGATCCAATTCACT
PRIMER_LEFT_14=855,20
PRIMER_RIGHT_14=1726,22
PRIMER_LEFT_14_TM=59.745
PRIMER_RIGHT_14_TM=58.201
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.4000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=872
PRIMER_PAIR_15_PENALTY=4.133573
PRIMER_LEFT_15_PENALTY=0.255244
PRIMER_RIGHT_15_PENALTY=3.878330
PRIMER_LEFT_15_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_15_SEQUENCE=GTAGTGGCAGATCCAATTCAC
PRIMER_LEFT_15=855,20
PRIMER_RIGHT_15=1726,21
PRIMER_LEFT_15_TM=59.745
PRIMER_RIGHT_15_TM=57.122
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=872
PRIMER_PAIR_16_PENALTY=4.145371
PRIMER_LEFT_16_PENALTY=0.255244
PRIMER_RIGHT_16_PENALTY=3.890127
PRIMER_LEFT_16_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_16_SEQUENCE=TAGTGGCAGATCCAATTCAC
PRIMER_LEFT_16=855,20
PRIMER_RIGHT_16=1725,20
PRIMER_LEFT_16_TM=59.745
PRIMER_RIGHT_16_TM=56.110
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.4000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=871
PRIMER_PAIR_17_PENALTY=4.145371
PRIMER_LEFT_17_PENALTY=0.255244
PRIMER_RIGHT_17_PENALTY=3.890127
PRIMER_LEFT_17_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_17_SEQUENCE=GTAGTGGCAGATCCAATTCA
PRIMER_LEFT_17=855,20
PRIMER_RIGHT_17=1726,20
PRIMER_LEFT_17_TM=59.745
PRIMER_RIGHT_17_TM=56.110
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.4000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=872
PRIMER_PAIR_18_PENALTY=4.173340
PRIMER_LEFT_18_PENALTY=0.255244
PRIMER_RIGHT_18_PENALTY=3.918097
PRIMER_LEFT_18_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_18_SEQUENCE=TGTAGTGGCAGATCCAATTCACT
PRIMER_LEFT_18=855,20
PRIMER_RIGHT_18=1727,23
PRIMER_LEFT_18_TM=59.745
PRIMER_RIGHT_18_TM=60.918
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.4000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=873
PRIMER_PAIR_19_PENALTY=4.185871
PRIMER_LEFT_19_PENALTY=1.043090
PRIMER_RIGHT_19_PENALTY=3.142781
PRIMER_LEFT_19_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_19_SEQUENCE=CCCTCAAGTTTTGTTTCGTT
PRIMER_LEFT_19=854,21
PRIMER_RIGHT_19=1587,20
PRIMER_LEFT_19_TM=60.043
PRIMER_RIGHT_19_TM=56.857
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.4000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=734
PRIMER_PAIR_20_PENALTY=4.275802
PRIMER_LEFT_20_PENALTY=1.043090
PRIMER_RIGHT_20_PENALTY=3.232712
PRIMER_LEFT_20_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_20_SEQUENCE=AAAAGTGTAGTGGCAGATCCAA
PRIMER_LEFT_20=854,21
PRIMER_RIGHT_20=1732,22
PRIMER_LEFT_20_TM=60.043
PRIMER_RIGHT_20_TM=58.767
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.4000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=879
PRIMER_PAIR_21_PENALTY=4.279508
PRIMER_LEFT_21_PENALTY=1.043090
PRIMER_RIGHT_21_PENALTY=3.236418
PRIMER_LEFT_21_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_21_SEQUENCE=CTCCACTGATTGGCTTAATTTG
PRIMER_LEFT_21=854,21
PRIMER_RIGHT_21=1689,22
PRIMER_LEFT_21_TM=60.043
PRIMER_RIGHT_21_TM=58.764
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.4000
PRIMER_RIGHT_21_END_STABILITY=7.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=836
PRIMER_PAIR_22_PENALTY=4.355299
PRIMER_LEFT_22_PENALTY=1.212518
PRIMER_RIGHT_22_PENALTY=3.142781
PRIMER_LEFT_22_SEQUENCE=GAACCAGCCTGTGACTGTTGT
PRIMER_RIGHT_22_SEQUENCE=CCCTCAAGTTTTGTTTCGTT
PRIMER_LEFT_22=723,21
PRIMER_RIGHT_22=1587,20
PRIMER_LEFT_22_TM=60.213
PRIMER_RIGHT_22_TM=56.857
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=865
PRIMER_PAIR_23_PENALTY=4.417982
PRIMER_LEFT_23_PENALTY=0.255244
PRIMER_RIGHT_23_PENALTY=4.162738
PRIMER_LEFT_23_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_23_SEQUENCE=CCACTGATTGGCTTAATTTG
PRIMER_LEFT_23=855,20
PRIMER_RIGHT_23=1687,20
PRIMER_LEFT_23_TM=59.745
PRIMER_RIGHT_23_TM=55.837
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.4000
PRIMER_RIGHT_23_END_STABILITY=7.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=833
PRIMER_PAIR_24_PENALTY=4.448936
PRIMER_LEFT_24_PENALTY=1.212518
PRIMER_RIGHT_24_PENALTY=3.236418
PRIMER_LEFT_24_SEQUENCE=GAACCAGCCTGTGACTGTTGT
PRIMER_RIGHT_24_SEQUENCE=CTCCACTGATTGGCTTAATTTG
PRIMER_LEFT_24=723,21
PRIMER_RIGHT_24=1689,22
PRIMER_LEFT_24_TM=60.213
PRIMER_RIGHT_24_TM=58.764
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=967
PRIMER_PAIR_25_PENALTY=4.492947
PRIMER_LEFT_25_PENALTY=1.350166
PRIMER_RIGHT_25_PENALTY=3.142781
PRIMER_LEFT_25_SEQUENCE=AGAACCAGCCTGTGACTGTTG
PRIMER_RIGHT_25_SEQUENCE=CCCTCAAGTTTTGTTTCGTT
PRIMER_LEFT_25=722,21
PRIMER_RIGHT_25=1587,20
PRIMER_LEFT_25_TM=60.350
PRIMER_RIGHT_25_TM=56.857
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=866
PRIMER_PAIR_26_PENALTY=4.501746
PRIMER_LEFT_26_PENALTY=0.255244
PRIMER_RIGHT_26_PENALTY=4.246502
PRIMER_LEFT_26_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_26_SEQUENCE=AAGTGTAGTGGCAGATCCAA
PRIMER_LEFT_26=855,20
PRIMER_RIGHT_26=1730,20
PRIMER_LEFT_26_TM=59.745
PRIMER_RIGHT_26_TM=55.753
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.4000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=876
PRIMER_PAIR_27_PENALTY=4.531963
PRIMER_LEFT_27_PENALTY=0.255244
PRIMER_RIGHT_27_PENALTY=4.276719
PRIMER_LEFT_27_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_27_SEQUENCE=CTAAAAGTGTAGTGGCAGATCCAA
PRIMER_LEFT_27=855,20
PRIMER_RIGHT_27=1734,24
PRIMER_LEFT_27_TM=59.745
PRIMER_RIGHT_27_TM=59.723
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=41.667
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.4000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=880
PRIMER_PAIR_28_PENALTY=4.565629
PRIMER_LEFT_28_PENALTY=1.043090
PRIMER_RIGHT_28_PENALTY=3.522538
PRIMER_LEFT_28_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_28_SEQUENCE=CATGCTAAAAGTGTAGTGGCAGA
PRIMER_LEFT_28=854,21
PRIMER_RIGHT_28=1738,23
PRIMER_LEFT_28_TM=60.043
PRIMER_RIGHT_28_TM=59.477
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.4000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=885
PRIMER_PAIR_29_PENALTY=4.565629
PRIMER_LEFT_29_PENALTY=1.043090
PRIMER_RIGHT_29_PENALTY=3.522538
PRIMER_LEFT_29_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_29_SEQUENCE=TCATGCTAAAAGTGTAGTGGCAG
PRIMER_LEFT_29=854,21
PRIMER_RIGHT_29=1739,23
PRIMER_LEFT_29_TM=60.043
PRIMER_RIGHT_29_TM=59.477
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=43.478
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.4000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=886
PRIMER_PAIR_30_PENALTY=4.570556
PRIMER_LEFT_30_PENALTY=0.255244
PRIMER_RIGHT_30_PENALTY=4.315313
PRIMER_LEFT_30_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_30_SEQUENCE=CATGCTAAAAGTGTAGTGGCAG
PRIMER_LEFT_30=855,20
PRIMER_RIGHT_30=1738,22
PRIMER_LEFT_30_TM=59.745
PRIMER_RIGHT_30_TM=57.685
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.4000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=884
PRIMER_PAIR_31_PENALTY=4.586584
PRIMER_LEFT_31_PENALTY=1.350166
PRIMER_RIGHT_31_PENALTY=3.236418
PRIMER_LEFT_31_SEQUENCE=AGAACCAGCCTGTGACTGTTG
PRIMER_RIGHT_31_SEQUENCE=CTCCACTGATTGGCTTAATTTG
PRIMER_LEFT_31=722,21
PRIMER_RIGHT_31=1689,22
PRIMER_LEFT_31_TM=60.350
PRIMER_RIGHT_31_TM=58.764
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=7.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=968
PRIMER_PAIR_32_PENALTY=4.699089
PRIMER_LEFT_32_PENALTY=1.043090
PRIMER_RIGHT_32_PENALTY=3.655999
PRIMER_LEFT_32_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_32_SEQUENCE=AAAGTGTAGTGGCAGATCCAA
PRIMER_LEFT_32=854,21
PRIMER_RIGHT_32=1731,21
PRIMER_LEFT_32_TM=60.043
PRIMER_RIGHT_32_TM=57.344
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.4000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=878
PRIMER_PAIR_33_PENALTY=4.761714
PRIMER_LEFT_33_PENALTY=1.043090
PRIMER_RIGHT_33_PENALTY=3.718624
PRIMER_LEFT_33_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_33_SEQUENCE=TAGTGGCAGATCCAATTCACT
PRIMER_LEFT_33=854,21
PRIMER_RIGHT_33=1725,21
PRIMER_LEFT_33_TM=60.043
PRIMER_RIGHT_33_TM=57.281
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.4000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=872
PRIMER_PAIR_34_PENALTY=4.842553
PRIMER_LEFT_34_PENALTY=1.043090
PRIMER_RIGHT_34_PENALTY=3.799463
PRIMER_LEFT_34_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_34_SEQUENCE=GTAGTGGCAGATCCAATTCACT
PRIMER_LEFT_34=854,21
PRIMER_RIGHT_34=1726,22
PRIMER_LEFT_34_TM=60.043
PRIMER_RIGHT_34_TM=58.201
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.4000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=873
PRIMER_PAIR_35_PENALTY=4.852824
PRIMER_LEFT_35_PENALTY=0.690085
PRIMER_RIGHT_35_PENALTY=4.162738
PRIMER_LEFT_35_SEQUENCE=GAACCAGCCTGTGACTGTTG
PRIMER_RIGHT_35_SEQUENCE=CCACTGATTGGCTTAATTTG
PRIMER_LEFT_35=723,20
PRIMER_RIGHT_35=1687,20
PRIMER_LEFT_35_TM=59.310
PRIMER_RIGHT_35_TM=55.837
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=965
PRIMER_PAIR_36_PENALTY=4.906422
PRIMER_LEFT_36_PENALTY=2.207976
PRIMER_RIGHT_36_PENALTY=2.698446
PRIMER_LEFT_36_SEQUENCE=CAGCCTGTGACTGTTGTCATT
PRIMER_RIGHT_36_SEQUENCE=TCCCTCAAGTTTTGTTTCGT
PRIMER_LEFT_36=727,21
PRIMER_RIGHT_36=1588,20
PRIMER_LEFT_36_TM=58.792
PRIMER_RIGHT_36_TM=57.302
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=8.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=862
PRIMER_PAIR_37_PENALTY=4.921420
PRIMER_LEFT_37_PENALTY=1.043090
PRIMER_RIGHT_37_PENALTY=3.878330
PRIMER_LEFT_37_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_37_SEQUENCE=GTAGTGGCAGATCCAATTCAC
PRIMER_LEFT_37=854,21
PRIMER_RIGHT_37=1726,21
PRIMER_LEFT_37_TM=60.043
PRIMER_RIGHT_37_TM=57.122
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.4000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=873
PRIMER_PAIR_38_PENALTY=4.929438
PRIMER_LEFT_38_PENALTY=0.255244
PRIMER_RIGHT_38_PENALTY=4.674194
PRIMER_LEFT_38_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_38_SEQUENCE=TGCTAAAAGTGTAGTGGCAGA
PRIMER_LEFT_38=855,20
PRIMER_RIGHT_38=1736,21
PRIMER_LEFT_38_TM=59.745
PRIMER_RIGHT_38_TM=56.326
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.4000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=882
PRIMER_PAIR_39_PENALTY=4.933218
PRIMER_LEFT_39_PENALTY=1.043090
PRIMER_RIGHT_39_PENALTY=3.890127
PRIMER_LEFT_39_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_39_SEQUENCE=TAGTGGCAGATCCAATTCAC
PRIMER_LEFT_39=854,21
PRIMER_RIGHT_39=1725,20
PRIMER_LEFT_39_TM=60.043
PRIMER_RIGHT_39_TM=56.110
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.4000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=872
PRIMER_PAIR_40_PENALTY=4.933218
PRIMER_LEFT_40_PENALTY=1.043090
PRIMER_RIGHT_40_PENALTY=3.890127
PRIMER_LEFT_40_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_40_SEQUENCE=GTAGTGGCAGATCCAATTCA
PRIMER_LEFT_40=854,21
PRIMER_RIGHT_40=1726,20
PRIMER_LEFT_40_TM=60.043
PRIMER_RIGHT_40_TM=56.110
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.4000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=873
PRIMER_PAIR_41_PENALTY=4.945060
PRIMER_LEFT_41_PENALTY=0.255244
PRIMER_RIGHT_41_PENALTY=4.689817
PRIMER_LEFT_41_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_41_SEQUENCE=TTCATGCTAAAAGTGTAGTGGCAG
PRIMER_LEFT_41=855,20
PRIMER_RIGHT_41=1740,24
PRIMER_LEFT_41_TM=59.745
PRIMER_RIGHT_41_TM=60.690
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=41.667
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.4000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=886
PRIMER_PAIR_42_PENALTY=4.961187
PRIMER_LEFT_42_PENALTY=1.043090
PRIMER_RIGHT_42_PENALTY=3.918097
PRIMER_LEFT_42_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_42_SEQUENCE=TGTAGTGGCAGATCCAATTCACT
PRIMER_LEFT_42=854,21
PRIMER_RIGHT_42=1727,23
PRIMER_LEFT_42_TM=60.043
PRIMER_RIGHT_42_TM=60.918
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.4000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=874
PRIMER_PAIR_43_PENALTY=5.049776
PRIMER_LEFT_43_PENALTY=2.207976
PRIMER_RIGHT_43_PENALTY=2.841800
PRIMER_LEFT_43_SEQUENCE=CAGCCTGTGACTGTTGTCATT
PRIMER_RIGHT_43_SEQUENCE=AGTGGCAGATCCAATTCACT
PRIMER_LEFT_43=727,21
PRIMER_RIGHT_43=1724,20
PRIMER_LEFT_43_TM=58.792
PRIMER_RIGHT_43_TM=57.158
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=998
PRIMER_PAIR_44_PENALTY=5.205829
PRIMER_LEFT_44_PENALTY=1.043090
PRIMER_RIGHT_44_PENALTY=4.162738
PRIMER_LEFT_44_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_44_SEQUENCE=CCACTGATTGGCTTAATTTG
PRIMER_LEFT_44=854,21
PRIMER_RIGHT_44=1687,20
PRIMER_LEFT_44_TM=60.043
PRIMER_RIGHT_44_TM=55.837
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.4000
PRIMER_RIGHT_44_END_STABILITY=7.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=834
PRIMER_PAIR_45_PENALTY=5.289592
PRIMER_LEFT_45_PENALTY=1.043090
PRIMER_RIGHT_45_PENALTY=4.246502
PRIMER_LEFT_45_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_45_SEQUENCE=AAGTGTAGTGGCAGATCCAA
PRIMER_LEFT_45=854,21
PRIMER_RIGHT_45=1730,20
PRIMER_LEFT_45_TM=60.043
PRIMER_RIGHT_45_TM=55.753
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.4000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=877
PRIMER_PAIR_46_PENALTY=5.350757
PRIMER_LEFT_46_PENALTY=2.207976
PRIMER_RIGHT_46_PENALTY=3.142781
PRIMER_LEFT_46_SEQUENCE=CAGCCTGTGACTGTTGTCATT
PRIMER_RIGHT_46_SEQUENCE=CCCTCAAGTTTTGTTTCGTT
PRIMER_LEFT_46=727,21
PRIMER_RIGHT_46=1587,20
PRIMER_LEFT_46_TM=58.792
PRIMER_RIGHT_46_TM=56.857
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=861
PRIMER_PAIR_47_PENALTY=5.358403
PRIMER_LEFT_47_PENALTY=1.043090
PRIMER_RIGHT_47_PENALTY=4.315313
PRIMER_LEFT_47_SEQUENCE=ATCGACCACAACCAGACGTAG
PRIMER_RIGHT_47_SEQUENCE=CATGCTAAAAGTGTAGTGGCAG
PRIMER_LEFT_47=854,21
PRIMER_RIGHT_47=1738,22
PRIMER_LEFT_47_TM=60.043
PRIMER_RIGHT_47_TM=57.685
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.4000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=885
PRIMER_PAIR_48_PENALTY=5.375257
PRIMER_LEFT_48_PENALTY=1.212518
PRIMER_RIGHT_48_PENALTY=4.162738
PRIMER_LEFT_48_SEQUENCE=GAACCAGCCTGTGACTGTTGT
PRIMER_RIGHT_48_SEQUENCE=CCACTGATTGGCTTAATTTG
PRIMER_LEFT_48=723,21
PRIMER_RIGHT_48=1687,20
PRIMER_LEFT_48_TM=60.213
PRIMER_RIGHT_48_TM=55.837
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=7.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=965
PRIMER_PAIR_49_PENALTY=5.425316
PRIMER_LEFT_49_PENALTY=0.255244
PRIMER_RIGHT_49_PENALTY=5.170072
PRIMER_LEFT_49_SEQUENCE=TCGACCACAACCAGACGTAG
PRIMER_RIGHT_49_SEQUENCE=TCATGCTAAAAGTGTAGTGGCAGA
PRIMER_LEFT_49=855,20
PRIMER_RIGHT_49=1739,24
PRIMER_LEFT_49_TM=59.745
PRIMER_RIGHT_49_TM=61.170
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=41.667
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.4000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=885
=
SEQUENCE_ID=MAGIC.329_3_14527619_14528325_wil-2_sf-2_43307_5437_TRUE
SEQUENCE_TARGET=950,806
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_19846915_19847510_po-0_sf-2_255404_909410_TRUE
SEQUENCE_TARGET=950,695
SEQUENCE_TEMPLATE=GATTTTGATATCATATATTGAATTAAAATTTCAAATTCTATCAAAATTCATGNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANTAACACATCNNNNNNNNNNNNCAAATCGTACTAAAGGCCCAGTAAGTCTTTTTGTCAATAAGTCCATTAAGCCCAACANACCTCTTCTTCTCTTTCNTTTTNTNACATCAATCCANTNNAAGCANACAAATCAGACGTAACCGAANNNAAAAAAAANGTTGNTTTGATCGNTCGTTAAAACCTAAGAAATGGATCCAAAATCGCCGGAATTTATCATTGATCAGCCGCTNAAAATCTTAGNATTTGTATTCGATGAGCTCTCCGCCACCAGAGTCTCCGGCCACCTCACCCTAACTGAAAAATGTTGCCAGCCGTTCAAAGTCTTGCACGGCGGCGTATCGGCTCTGATCGCTGAGGCACTCGCCAGTCTCGGCGCCGGAATCGCCTCTGGTTTTAAACGCGTCGCTGGAATCCATCTCTCTATTCACCACCTCCGTCCTGCTGCTCTCGGCGANNTNGTCTTCGCCGAATCTTTTCCAGTCTCNGTCGGCAAAAATATTCAGGTAACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAAATTTCCGGCAAGCAAAAGCAGCTCNTGTACCAAGTTGTCGGAAACTTNANTTTTGTTAGCATGCATTGGANTAAACCAACGCAAATATTTTATTTTATTTATCGTAGACTGAAGACCTAAAGAGTAAAATGAATGTGAAAGTGACACGTTGTGTGGAGTATTTTGTATCTAACTAGGTGTGGGAGGTCCGGTTATGGAAAGCTAAGAAAACCGAGACACCGGACAACAAAATAATGGTATCAACATCACGGGTTACTCTCTTCTGCGGTTTACCTATACCGGACCATGTTAAAGATGCTCCAGACGAGCTGAAGAAAGTTATATCCAAANTATNAATTCAAATTGTATCTATNTCATTTTGTTNGGAATAAGAACAATTTTANTTTGGTTTNATTGTANGAACCAAACGTTNTAATAAATTGTTGTACCAAACATGAGNTATTATAAGGTTATAAAAATTGACAAGTTATTGTGGGTTTAGTTTGTAGATCATTTTGAAATNTAGTATTAATAGACNATNTTGTTGAAACGTCCAAATCNTATAGATGGGGTAANNAAACTAAACTCNTTNATATGATTCTTTTGCATAAAATTNTGTCTTTGTAATAACTGAATCATACNTANTTTTTTNGTTTGATACNCATNNNNNAATTACCACAACTATGTAGNGCATTTAAGATATCTTTCTTCATATTATAAATGTGTATCAATACCATTTAAATCTTCGTATACAAAGAAAAAAANCAAGATGGATATTAGTTTTTCGATGGAGATATTATTTGGTTGAGTTCTTTCTTCTTGAGACAACAACATCNAGTGTGNAGTCTAAATGTTCCACAATACCATCCATTTGTATANCNGTGGCTTAAGCAAATCTGATCACATACGCTTGTACACTNGGACCNATAACACATGGCGTCATGAACGTCANTAGCGTTTATTTTANCTGTAAAAGATATCATAAAAATCTATTATATATGATAGATCATCATTTATGTTTATATTTAGAGAGCTCTACATNGAGATTCGATCACGATCAAACGNTAAGGTTACTTCACNATAAACTAACGAAGATAGTGTATTTTGCTGAATGATGTAAATTATANNAAAAAAAAATNTAAGGTATATATATAAACAAACGAAGGTAATATGGTTTAGATCAAGAAAATACCCTAATAGAAATCAAAAAGTTTGTGTATANGTTTTATGAATTTGTAAATTGACTTACCGGCCATCGCAAACAAGAGAANAATTGTTATGGCAGTNAGTATATGTGAATGTTTCTGGTTCTGCNTTTTTTTNTTTTGAGGTGTCACTAGCTAATAGCTATTACTTTGTTTTCTCNTTGTTTTTAGATGTATCCANTGAGATTAAAGTGCTCTTATATAGGGGTTTGGTTTTCNCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_5_15606032_15606506_zu-0_ct-1_159409_3831133_TRUE
SEQUENCE_TARGET=950,574
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCAATCANNNCTCAANCAAATCACTCCTCACACCACAGAAANACAAATAATTNAAGAAATGAAGTCAATAGTCTTCTTCTTCCTCCTCCTGCTCTTTGCCGTAGCAACCTCCGATAACGTGATAAATGGTTGGTATAATCTCACAGCCGTCCATGACGATGAAGTGATTTGGAGTTCTTTTGCGGCGCCAATGATGAAAGGTGGTTCGTCGTACATAACCTATCCGGTCTTGCGACCTCAGCAGGCATGTAATCCTTGTAGTGGANCCGTTAAATATNCCAANAAGAAGAATTGTCTTGNCTACACCAGATGCAAACGCNNTCNTAANGGCNTGTAACATACNCCNNNTANATTCTCNATGTTTCNGTTTGTGTCGTTCTAAGCGTTTGGGAAATAACTTGCTTGTAACNAAANGTACGGTGTCTNTTTGTTGCCAAATTATNGAGTGTTGTTTCTTAATAATNTTCNTTAAAGTCAGCGAAGTAATTATTCTATAAAAACTAAGAGTTGCTCTATCTAGATATATATACAATTGTGGACTACTTCTTTAAAAATTAAATAAATTGGTAAAGTTNAAAGGTACGTCTCCCAAGCAGAAACTGAACTAAAGAATTCAGGNNNNNACTCCTNTGAAGGGAAACACTCAAAAAACAAAAANAAAAAAAGATTCATTTGGTAAACATAATAATAGTGAAGANAATATAATATGCCAAAAGTGGGTTTTGAACTCATGTCCT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_5185501_5185840_bur-0_po-0_2824_12789_TRUE
SEQUENCE_TARGET=950,439
SEQUENCE_TEMPLATE=GANGCTAAACTNNAACANACTTTNAGAATATTTGAGACGATNAACTGGTCNGCATCGAGTCCCAACTTGNTTAATTCNCTGAANAGATAAAATGCTAAAGAATTGAACCCTGATTTCACACANCCTCGTATCAAGCCAGAGAAAGCGATGATATCTTTATTTGGAAGCNNATGAAACAGNTTATGTGCNTCTTGTATATTCCCAACNTTTGCNTGTANATCAACAAGAATNCTTCCTACNATGTAATCCAACTCATAACCACTTACTACAACTAAACTATGTACTTGAAGCCCGAGTCTTAAGTTGACNNAGTTGATACATATCTTTAAGGCACCACTTAGAGTGTAGGAATCAAAANANAAATCTGACTGANANATCTGCAAAAGNAGCCNTAATGCTGCTTCATTCTCNTCNTTNATCAAAAATCCAGAGAGCATAGAATTCCAAACNGNGANACTANTANNNNNNNNNNGNTTTTCCTGATGAAAAACNTCAGCTGCATNNNTTAGAGANCCACAGTTNGAGTACATATCAATAAGAGCAGANATTGCGAACGGGCTAGACTCTAGACCCGACTTTNCGACACAACAATGCAGCTGTTTACCCATTGTTAATAANCCACCAAAACTACANGCTTTAAGACCACANGGTAAAGCAAAACCGTCTAACACAAGNCCTTCTCTCTGCATCNNAACTAAAAACTCCAACGCACNAGGACTACCCTTATCGACAAATCCCGATANCAAACAGTTCCAAGACACGACATTTGGCNGCGGCATTNGATGNAACAAANTAACNGCTTCATCNATCAAACCAGCTTTACAATAACCAGAAATAAGAGTGTTCCACGAAGTCGAACTAGGCCGTAAAATCTCCTTAAAANTGCTANTAGCTTCNNTAAGTCTCCCATTCTTAACATACATATCNACAACACTATTCATCAAAACAACATCACCNCTCAAATTCTCTTNACCAATTCTTNCATAAACCAAANTCCCNANCTGAATATCACCCACTAACCCACAAGCTTTCAAAACCGCAGAATACATAAACTCGTTCGCNGCTTCTTCTTCGGAGTCTAACATTCTTCTGTATAACTCAATAGCTTTATTGGGTTTTCCATCACTCGTATACCCAGAAACCATCGTTGTCCAAGTTACAATATTTCTCTCANACATTTCNTCGAACACTTTATGTGCATCACTCAACAANCTNAAATCCACATACATTGAGATCACGTTATTCGCTATAAACACATTTTGAGAGATCCCTTGCTTGATTACATGAGCTTGANTTGATTCTCCACGCTTAAAGGCTTGTACTTTCCCACAATGACGTANTCCTGCTGCTATANGCTTCAAGTCCANAACCATTCTCCAGTANGAAAATTTTNGACAATNNCGTTACATTTACAAATTGNNTANTCTCCACGTAAAACGGAAGGTAGTTTTATNGAAAANNTNAAAACGGCACGCTGTTTAGTACATGAATTNANGACNGTANAAACGGTNGGTAGTTTGNTTGGTAANNNGNAAACNGCANGCTGTTCAATACGNTAGTGGCTGNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNAANNNNTTNNCNNNNNNNGTAAAAACGGAAGGTNGTTTNTTTNAGAAAATAAAAACGTTACNNTGNTNANTACNNNANGGAAGATNGTAAAACGNCACNATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTGTGTTGTGGAAATTCAAAACGGCACNNTGTTNAACATNGGNGTGAAGAANAATCTCATAATTTAGGATCAATGGCTGATNAAAAAAAAANCAGATCTNAAANNNNNTCCGTCGTCGCCGTCTTCCTTCACGANNNNCTCCTCTCTTCCNAAACTTCAATCTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.904112
PRIMER_LEFT_0_PENALTY=0.801088
PRIMER_RIGHT_0_PENALTY=0.103024
PRIMER_LEFT_0_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_0_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_0=591,20
PRIMER_RIGHT_0=1443,20
PRIMER_LEFT_0_TM=59.199
PRIMER_RIGHT_0_TM=60.103
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=853
PRIMER_PAIR_1_PENALTY=1.113553
PRIMER_LEFT_1_PENALTY=1.010529
PRIMER_RIGHT_1_PENALTY=0.103024
PRIMER_LEFT_1_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_1_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_1=588,21
PRIMER_RIGHT_1=1443,20
PRIMER_LEFT_1_TM=60.011
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=1.590253
PRIMER_LEFT_2_PENALTY=1.487228
PRIMER_RIGHT_2_PENALTY=0.103024
PRIMER_LEFT_2_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_2_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_2=590,21
PRIMER_RIGHT_2=1443,20
PRIMER_LEFT_2_TM=59.513
PRIMER_RIGHT_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=854
PRIMER_PAIR_3_PENALTY=1.651962
PRIMER_LEFT_3_PENALTY=1.548938
PRIMER_RIGHT_3_PENALTY=0.103024
PRIMER_LEFT_3_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_3_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_3=585,21
PRIMER_RIGHT_3=1443,20
PRIMER_LEFT_3_TM=60.549
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=859
PRIMER_PAIR_4_PENALTY=2.348109
PRIMER_LEFT_4_PENALTY=2.245085
PRIMER_RIGHT_4_PENALTY=0.103024
PRIMER_LEFT_4_SEQUENCE=AACAATGCAGCTGTTTACCC
PRIMER_RIGHT_4_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_4=586,20
PRIMER_RIGHT_4=1443,20
PRIMER_LEFT_4_TM=57.755
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=858
PRIMER_PAIR_5_PENALTY=2.575323
PRIMER_LEFT_5_PENALTY=2.472299
PRIMER_RIGHT_5_PENALTY=0.103024
PRIMER_LEFT_5_SEQUENCE=GAAGTCGAACTAGGCCGTAAA
PRIMER_RIGHT_5_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_5=849,21
PRIMER_RIGHT_5=1443,20
PRIMER_LEFT_5_TM=58.528
PRIMER_RIGHT_5_TM=60.103
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.0000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=595
PRIMER_PAIR_6_PENALTY=2.690022
PRIMER_LEFT_6_PENALTY=0.801088
PRIMER_RIGHT_6_PENALTY=1.888934
PRIMER_LEFT_6_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_6_SEQUENCE=ACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_6=591,20
PRIMER_RIGHT_6=1444,21
PRIMER_LEFT_6_TM=59.199
PRIMER_RIGHT_6_TM=60.889
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=854
PRIMER_PAIR_7_PENALTY=2.698281
PRIMER_LEFT_7_PENALTY=2.595257
PRIMER_RIGHT_7_PENALTY=0.103024
PRIMER_LEFT_7_SEQUENCE=CACAACAATGCAGCTGTTTACC
PRIMER_RIGHT_7_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_7=583,22
PRIMER_RIGHT_7=1443,20
PRIMER_LEFT_7_TM=60.595
PRIMER_RIGHT_7_TM=60.103
PRIMER_LEFT_7_GC_PERCENT=45.455
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.2000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=861
PRIMER_PAIR_8_PENALTY=2.827622
PRIMER_LEFT_8_PENALTY=2.724598
PRIMER_RIGHT_8_PENALTY=0.103024
PRIMER_LEFT_8_SEQUENCE=CAAACAGTTCCAAGACACGA
PRIMER_RIGHT_8_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_8=743,20
PRIMER_RIGHT_8=1443,20
PRIMER_LEFT_8_TM=57.275
PRIMER_RIGHT_8_TM=60.103
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=701
PRIMER_PAIR_9_PENALTY=2.845698
PRIMER_LEFT_9_PENALTY=2.742674
PRIMER_RIGHT_9_PENALTY=0.103024
PRIMER_LEFT_9_SEQUENCE=AAACAGTTCCAAGACACGACA
PRIMER_RIGHT_9_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_9=744,21
PRIMER_RIGHT_9=1443,20
PRIMER_LEFT_9_TM=58.257
PRIMER_RIGHT_9_TM=60.103
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=700
PRIMER_PAIR_10_PENALTY=2.845698
PRIMER_LEFT_10_PENALTY=2.742674
PRIMER_RIGHT_10_PENALTY=0.103024
PRIMER_LEFT_10_SEQUENCE=CAAACAGTTCCAAGACACGAC
PRIMER_RIGHT_10_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_10=743,21
PRIMER_RIGHT_10=1443,20
PRIMER_LEFT_10_TM=58.257
PRIMER_RIGHT_10_TM=60.103
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.8000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=701
PRIMER_PAIR_11_PENALTY=2.860515
PRIMER_LEFT_11_PENALTY=2.757491
PRIMER_RIGHT_11_PENALTY=0.103024
PRIMER_LEFT_11_SEQUENCE=AATGCAGCTGTTTACCCATT
PRIMER_RIGHT_11_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_11=589,20
PRIMER_RIGHT_11=1443,20
PRIMER_LEFT_11_TM=57.243
PRIMER_RIGHT_11_TM=60.103
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=855
PRIMER_PAIR_12_PENALTY=2.881550
PRIMER_LEFT_12_PENALTY=2.778526
PRIMER_RIGHT_12_PENALTY=0.103024
PRIMER_LEFT_12_SEQUENCE=AATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_12_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_12=589,22
PRIMER_RIGHT_12=1443,20
PRIMER_LEFT_12_TM=60.779
PRIMER_RIGHT_12_TM=60.103
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=855
PRIMER_PAIR_13_PENALTY=2.881550
PRIMER_LEFT_13_PENALTY=2.778526
PRIMER_RIGHT_13_PENALTY=0.103024
PRIMER_LEFT_13_SEQUENCE=ACAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_13_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_13=587,22
PRIMER_RIGHT_13=1443,20
PRIMER_LEFT_13_TM=60.779
PRIMER_RIGHT_13_TM=60.103
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=857
PRIMER_PAIR_14_PENALTY=2.899463
PRIMER_LEFT_14_PENALTY=1.010529
PRIMER_RIGHT_14_PENALTY=1.888934
PRIMER_LEFT_14_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_14_SEQUENCE=ACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_14=588,21
PRIMER_RIGHT_14=1444,21
PRIMER_LEFT_14_TM=60.011
PRIMER_RIGHT_14_TM=60.889
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=857
PRIMER_PAIR_15_PENALTY=3.084620
PRIMER_LEFT_15_PENALTY=2.981596
PRIMER_RIGHT_15_PENALTY=0.103024
PRIMER_LEFT_15_SEQUENCE=TCGAACTAGGCCGTAAAATC
PRIMER_RIGHT_15_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_15=853,20
PRIMER_RIGHT_15=1443,20
PRIMER_LEFT_15_TM=57.018
PRIMER_RIGHT_15_TM=60.103
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=591
PRIMER_PAIR_16_PENALTY=3.141793
PRIMER_LEFT_16_PENALTY=3.038769
PRIMER_RIGHT_16_PENALTY=0.103024
PRIMER_LEFT_16_SEQUENCE=AAGTCGAACTAGGCCGTAAAATC
PRIMER_RIGHT_16_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_16=850,23
PRIMER_RIGHT_16=1443,20
PRIMER_LEFT_16_TM=60.039
PRIMER_RIGHT_16_TM=60.103
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=594
PRIMER_PAIR_17_PENALTY=3.191797
PRIMER_LEFT_17_PENALTY=3.088773
PRIMER_RIGHT_17_PENALTY=0.103024
PRIMER_LEFT_17_SEQUENCE=GACTACCCTTATCGACAAATCCC
PRIMER_RIGHT_17_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_17=715,23
PRIMER_RIGHT_17=1443,20
PRIMER_LEFT_17_TM=60.089
PRIMER_RIGHT_17_TM=60.103
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.3000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=729
PRIMER_PAIR_18_PENALTY=3.191797
PRIMER_LEFT_18_PENALTY=3.088773
PRIMER_RIGHT_18_PENALTY=0.103024
PRIMER_LEFT_18_SEQUENCE=GGACTACCCTTATCGACAAATCC
PRIMER_RIGHT_18_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_18=714,23
PRIMER_RIGHT_18=1443,20
PRIMER_LEFT_18_TM=60.089
PRIMER_RIGHT_18_TM=60.103
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=730
PRIMER_PAIR_19_PENALTY=3.203094
PRIMER_LEFT_19_PENALTY=3.100070
PRIMER_RIGHT_19_PENALTY=0.103024
PRIMER_LEFT_19_SEQUENCE=GTCGAACTAGGCCGTAAAATC
PRIMER_RIGHT_19_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_19=852,21
PRIMER_RIGHT_19=1443,20
PRIMER_LEFT_19_TM=57.900
PRIMER_RIGHT_19_TM=60.103
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=7.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=592
PRIMER_PAIR_20_PENALTY=3.227101
PRIMER_LEFT_20_PENALTY=3.124077
PRIMER_RIGHT_20_PENALTY=0.103024
PRIMER_LEFT_20_SEQUENCE=CAACAATGCAGCTGTTTACC
PRIMER_RIGHT_20_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_20=585,20
PRIMER_RIGHT_20=1443,20
PRIMER_LEFT_20_TM=56.876
PRIMER_RIGHT_20_TM=60.103
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=859
PRIMER_PAIR_21_PENALTY=3.255502
PRIMER_LEFT_21_PENALTY=3.152478
PRIMER_RIGHT_21_PENALTY=0.103024
PRIMER_LEFT_21_SEQUENCE=CAAACAGTTCCAAGACACGACA
PRIMER_RIGHT_21_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_21=743,22
PRIMER_RIGHT_21=1443,20
PRIMER_LEFT_21_TM=61.152
PRIMER_RIGHT_21_TM=60.103
PRIMER_LEFT_21_GC_PERCENT=45.455
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=701
PRIMER_PAIR_22_PENALTY=3.256086
PRIMER_LEFT_22_PENALTY=3.153061
PRIMER_RIGHT_22_PENALTY=0.103024
PRIMER_LEFT_22_SEQUENCE=AGTCGAACTAGGCCGTAAAATC
PRIMER_RIGHT_22_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_22=851,22
PRIMER_RIGHT_22=1443,20
PRIMER_LEFT_22_TM=58.847
PRIMER_RIGHT_22_TM=60.103
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.9000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=593
PRIMER_PAIR_23_PENALTY=3.277520
PRIMER_LEFT_23_PENALTY=3.174496
PRIMER_RIGHT_23_PENALTY=0.103024
PRIMER_LEFT_23_SEQUENCE=ACAACAATGCAGCTGTTTACC
PRIMER_RIGHT_23_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_23=584,21
PRIMER_RIGHT_23=1443,20
PRIMER_LEFT_23_TM=57.826
PRIMER_RIGHT_23_TM=60.103
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.2000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=860
PRIMER_PAIR_24_PENALTY=3.330339
PRIMER_LEFT_24_PENALTY=0.801088
PRIMER_RIGHT_24_PENALTY=2.529250
PRIMER_LEFT_24_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_24_SEQUENCE=AACTACCTTCCGTTTTACGTGG
PRIMER_LEFT_24=591,20
PRIMER_RIGHT_24=1448,22
PRIMER_LEFT_24_TM=59.199
PRIMER_RIGHT_24_TM=59.471
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=9.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=858
PRIMER_PAIR_25_PENALTY=3.376162
PRIMER_LEFT_25_PENALTY=1.487228
PRIMER_RIGHT_25_PENALTY=1.888934
PRIMER_LEFT_25_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_25_SEQUENCE=ACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_25=590,21
PRIMER_RIGHT_25=1444,21
PRIMER_LEFT_25_TM=59.513
PRIMER_RIGHT_25_TM=60.889
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=855
PRIMER_PAIR_26_PENALTY=3.407039
PRIMER_LEFT_26_PENALTY=3.304015
PRIMER_RIGHT_26_PENALTY=0.103024
PRIMER_LEFT_26_SEQUENCE=AAGTCGAACTAGGCCGTAAA
PRIMER_RIGHT_26_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_26=850,20
PRIMER_RIGHT_26=1443,20
PRIMER_LEFT_26_TM=56.696
PRIMER_RIGHT_26_TM=60.103
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.0000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=594
PRIMER_PAIR_27_PENALTY=3.410841
PRIMER_LEFT_27_PENALTY=3.307817
PRIMER_RIGHT_27_PENALTY=0.103024
PRIMER_LEFT_27_SEQUENCE=ACAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_27_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_27=584,22
PRIMER_RIGHT_27=1443,20
PRIMER_LEFT_27_TM=61.308
PRIMER_RIGHT_27_TM=60.103
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=860
PRIMER_PAIR_28_PENALTY=3.437872
PRIMER_LEFT_28_PENALTY=1.548938
PRIMER_RIGHT_28_PENALTY=1.888934
PRIMER_LEFT_28_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_28_SEQUENCE=ACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_28=585,21
PRIMER_RIGHT_28=1444,21
PRIMER_LEFT_28_TM=60.549
PRIMER_RIGHT_28_TM=60.889
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=860
PRIMER_PAIR_29_PENALTY=3.441125
PRIMER_LEFT_29_PENALTY=3.338101
PRIMER_RIGHT_29_PENALTY=0.103024
PRIMER_LEFT_29_SEQUENCE=AACAGTTCCAAGACACGACA
PRIMER_RIGHT_29_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_29=745,20
PRIMER_RIGHT_29=1443,20
PRIMER_LEFT_29_TM=56.662
PRIMER_RIGHT_29_TM=60.103
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=699
PRIMER_PAIR_30_PENALTY=3.476028
PRIMER_LEFT_30_PENALTY=3.373003
PRIMER_RIGHT_30_PENALTY=0.103024
PRIMER_LEFT_30_SEQUENCE=AACAGTTCCAAGACACGACATT
PRIMER_RIGHT_30_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_30=745,22
PRIMER_RIGHT_30=1443,20
PRIMER_LEFT_30_TM=58.627
PRIMER_RIGHT_30_TM=60.103
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=699
PRIMER_PAIR_31_PENALTY=3.490758
PRIMER_LEFT_31_PENALTY=0.801088
PRIMER_RIGHT_31_PENALTY=2.689670
PRIMER_LEFT_31_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_31_SEQUENCE=CTACCTTCCGTTTTACGTGG
PRIMER_LEFT_31=591,20
PRIMER_RIGHT_31=1446,20
PRIMER_LEFT_31_TM=59.199
PRIMER_RIGHT_31_TM=57.310
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=856
PRIMER_PAIR_32_PENALTY=3.536128
PRIMER_LEFT_32_PENALTY=3.433104
PRIMER_RIGHT_32_PENALTY=0.103024
PRIMER_LEFT_32_SEQUENCE=CTACCCTTATCGACAAATCCC
PRIMER_RIGHT_32_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_32=717,21
PRIMER_RIGHT_32=1443,20
PRIMER_LEFT_32_TM=57.567
PRIMER_RIGHT_32_TM=60.103
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.3000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=727
PRIMER_PAIR_33_PENALTY=3.539779
PRIMER_LEFT_33_PENALTY=1.010529
PRIMER_RIGHT_33_PENALTY=2.529250
PRIMER_LEFT_33_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_33_SEQUENCE=AACTACCTTCCGTTTTACGTGG
PRIMER_LEFT_33=588,21
PRIMER_RIGHT_33=1448,22
PRIMER_LEFT_33_TM=60.011
PRIMER_RIGHT_33_TM=59.471
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=9.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=861
PRIMER_PAIR_34_PENALTY=3.599954
PRIMER_LEFT_34_PENALTY=3.496930
PRIMER_RIGHT_34_PENALTY=0.103024
PRIMER_LEFT_34_SEQUENCE=TACCCTTATCGACAAATCCC
PRIMER_RIGHT_34_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_34=718,20
PRIMER_RIGHT_34=1443,20
PRIMER_LEFT_34_TM=56.503
PRIMER_RIGHT_34_TM=60.103
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.3000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=726
PRIMER_PAIR_35_PENALTY=3.604231
PRIMER_LEFT_35_PENALTY=3.501207
PRIMER_RIGHT_35_PENALTY=0.103024
PRIMER_LEFT_35_SEQUENCE=AAGCAAAACCGTCTAACACAAG
PRIMER_RIGHT_35_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_35=652,22
PRIMER_RIGHT_35=1443,20
PRIMER_LEFT_35_TM=58.499
PRIMER_RIGHT_35_TM=60.103
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=792
PRIMER_PAIR_36_PENALTY=3.613443
PRIMER_LEFT_36_PENALTY=0.801088
PRIMER_RIGHT_36_PENALTY=2.812355
PRIMER_LEFT_36_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_36_SEQUENCE=ACTACCTTCCGTTTTACGTGG
PRIMER_LEFT_36=591,20
PRIMER_RIGHT_36=1447,21
PRIMER_LEFT_36_TM=59.199
PRIMER_RIGHT_36_TM=58.188
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=9.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=857
PRIMER_PAIR_37_PENALTY=3.668507
PRIMER_LEFT_37_PENALTY=0.801088
PRIMER_RIGHT_37_PENALTY=2.867419
PRIMER_LEFT_37_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_37_SEQUENCE=TACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_37=591,20
PRIMER_RIGHT_37=1445,22
PRIMER_LEFT_37_TM=59.199
PRIMER_RIGHT_37_TM=60.867
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=855
PRIMER_PAIR_38_PENALTY=3.695350
PRIMER_LEFT_38_PENALTY=3.592326
PRIMER_RIGHT_38_PENALTY=0.103024
PRIMER_LEFT_38_SEQUENCE=ACTACCCTTATCGACAAATCCC
PRIMER_RIGHT_38_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_38=716,22
PRIMER_RIGHT_38=1443,20
PRIMER_LEFT_38_TM=58.408
PRIMER_RIGHT_38_TM=60.103
PRIMER_LEFT_38_GC_PERCENT=45.455
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.3000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=728
PRIMER_PAIR_39_PENALTY=3.700199
PRIMER_LEFT_39_PENALTY=1.010529
PRIMER_RIGHT_39_PENALTY=2.689670
PRIMER_LEFT_39_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_39_SEQUENCE=CTACCTTCCGTTTTACGTGG
PRIMER_LEFT_39=588,21
PRIMER_RIGHT_39=1446,20
PRIMER_LEFT_39_TM=60.011
PRIMER_RIGHT_39_TM=57.310
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.4000
PRIMER_RIGHT_39_END_STABILITY=9.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=859
PRIMER_PAIR_40_PENALTY=3.732318
PRIMER_LEFT_40_PENALTY=3.629294
PRIMER_RIGHT_40_PENALTY=0.103024
PRIMER_LEFT_40_SEQUENCE=GGTAAAGCAAAACCGTCTAACA
PRIMER_RIGHT_40_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_40=648,22
PRIMER_RIGHT_40=1443,20
PRIMER_LEFT_40_TM=58.371
PRIMER_RIGHT_40_TM=60.103
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.0000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=796
PRIMER_PAIR_41_PENALTY=3.822884
PRIMER_LEFT_41_PENALTY=1.010529
PRIMER_RIGHT_41_PENALTY=2.812355
PRIMER_LEFT_41_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_41_SEQUENCE=ACTACCTTCCGTTTTACGTGG
PRIMER_LEFT_41=588,21
PRIMER_RIGHT_41=1447,21
PRIMER_LEFT_41_TM=60.011
PRIMER_RIGHT_41_TM=58.188
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.4000
PRIMER_RIGHT_41_END_STABILITY=9.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=860
PRIMER_PAIR_42_PENALTY=3.868510
PRIMER_LEFT_42_PENALTY=3.765486
PRIMER_RIGHT_42_PENALTY=0.103024
PRIMER_LEFT_42_SEQUENCE=GAACTAGGCCGTAAAATCTCCTT
PRIMER_RIGHT_42_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_42=855,23
PRIMER_RIGHT_42=1443,20
PRIMER_LEFT_42_TM=59.235
PRIMER_RIGHT_42_TM=60.103
PRIMER_LEFT_42_GC_PERCENT=43.478
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=589
PRIMER_PAIR_43_PENALTY=3.877948
PRIMER_LEFT_43_PENALTY=1.010529
PRIMER_RIGHT_43_PENALTY=2.867419
PRIMER_LEFT_43_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_43_SEQUENCE=TACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_43=588,21
PRIMER_RIGHT_43=1445,22
PRIMER_LEFT_43_TM=60.011
PRIMER_RIGHT_43_TM=60.867
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=858
PRIMER_PAIR_44_PENALTY=3.958862
PRIMER_LEFT_44_PENALTY=3.855837
PRIMER_RIGHT_44_PENALTY=0.103024
PRIMER_LEFT_44_SEQUENCE=ACAGTTCCAAGACACGACATT
PRIMER_RIGHT_44_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_44=746,21
PRIMER_RIGHT_44=1443,20
PRIMER_LEFT_44_TM=57.144
PRIMER_RIGHT_44_TM=60.103
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.6000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=698
PRIMER_PAIR_45_PENALTY=3.989156
PRIMER_LEFT_45_PENALTY=3.886132
PRIMER_RIGHT_45_PENALTY=0.103024
PRIMER_LEFT_45_SEQUENCE=CAGTTCCAAGACACGACATT
PRIMER_RIGHT_45_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_45=747,20
PRIMER_RIGHT_45=1443,20
PRIMER_LEFT_45_TM=56.114
PRIMER_RIGHT_45_TM=60.103
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.6000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=697
PRIMER_PAIR_46_PENALTY=3.990497
PRIMER_LEFT_46_PENALTY=3.887472
PRIMER_RIGHT_46_PENALTY=0.103024
PRIMER_LEFT_46_SEQUENCE=AGCAAAACCGTCTAACACAAG
PRIMER_RIGHT_46_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_46=653,21
PRIMER_RIGHT_46=1443,20
PRIMER_LEFT_46_TM=57.113
PRIMER_RIGHT_46_TM=60.103
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=791
PRIMER_PAIR_47_PENALTY=4.016479
PRIMER_LEFT_47_PENALTY=1.487228
PRIMER_RIGHT_47_PENALTY=2.529250
PRIMER_LEFT_47_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_47_SEQUENCE=AACTACCTTCCGTTTTACGTGG
PRIMER_LEFT_47=590,21
PRIMER_RIGHT_47=1448,22
PRIMER_LEFT_47_TM=59.513
PRIMER_RIGHT_47_TM=59.471
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=859
PRIMER_PAIR_48_PENALTY=4.078188
PRIMER_LEFT_48_PENALTY=1.548938
PRIMER_RIGHT_48_PENALTY=2.529250
PRIMER_LEFT_48_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_48_SEQUENCE=AACTACCTTCCGTTTTACGTGG
PRIMER_LEFT_48=585,21
PRIMER_RIGHT_48=1448,22
PRIMER_LEFT_48_TM=60.549
PRIMER_RIGHT_48_TM=59.471
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=864
PRIMER_PAIR_49_PENALTY=4.115185
PRIMER_LEFT_49_PENALTY=4.012160
PRIMER_RIGHT_49_PENALTY=0.103024
PRIMER_LEFT_49_SEQUENCE=GCAAAACCGTCTAACACAAG
PRIMER_RIGHT_49_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_49=654,20
PRIMER_RIGHT_49=1443,20
PRIMER_LEFT_49_TM=55.988
PRIMER_RIGHT_49_TM=60.103
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=790
=
SEQUENCE_ID=MAGIC.105_4_9468942_9469162_po-0_sf-2_23319_6280_TRUE
SEQUENCE_TARGET=950,320
SEQUENCE_TEMPLATE=TCGGGTCTANATGGCGNGAGCTACACGGGTTAAANAACTGGGCCGGGTTGTTGGATCCNTTAGACGAGAATCTCCGGCGAGAGCTNGTCCGCTANGGAGAGTTTGTNCAAGCNGCTTATCACGCGTTTCACTCNGACCCGGAAGGTTCGCCGAGACACGTGGCGTTACCTGATGGGTCATTTAAAGTGACCAAGAGTCTTTACGCCACGTCATCCGTTCGTTTACCTAANTGGATCGATGACGTTGCACCNGATCTNCGNTGGATGACTAAACAGACGAGCTGGGTTGGTTACGTGGCGGTGTGTGATGATCCGAGAGAGATCNGACGGATGGGAAGGAGGGAGATCGTCATCGCNCTCCGTGGAACCGCCACGTTGCTCGAATGGTCAGANAATTTCAGGCCCAATCTCGTTTCAATGCCTGAGCCCAAACCCGATCAATCCGACCCGACTCGACCNAAAGTGGAATGCGGATTTAATAGCCTCTACACGACGGGTNNCCAANACGCACCNAGNCTNGCCGAGTCNTTGGTCGGNGAAATCAGCCGNCTCGTNGANCTCTANGCAGGAGAGGAGCTGAGNATNAGCGTNACCGGCCACAGCCTNGGNGCCGCAATNGCNCTTCTNGCCGCCGANGACATCNCCGAGCGTGTTCCACACGCNCCTCCGGTTGCGNTGTTTTCCTTNGGNGGNCCTCGAGTCGGGAANCGTGAGTTNGCNGATNGNNTNGATTCGAANGGAGTGAAAGTCCTNCGCGTNGTNAACAGCCAAGANGTGGTNACGAAAGTNCCCGGAATATTCNCCGATAANGATAANCAGGGGCAANNTCGTAATAACGGGAGAAGTCCNGGNGGTATNATGGAAATGGTGGAGAGGAACAATCCATGGGCTTACTCNCATGTGGGAGCAGAGCTNCGNGTAGACATGAAGATGTCTCCGTACTTGAAACCAAACGCTGACGTGGCGTGTTGNCATGACCTTGAGGCNTACTTGCACTTAGTNGACGGNTTTTTGGCATCTAACTGTCCGTTTCGGGCAAACGCGAAACGGAGTTTAAGGAANCTGTTGGATGAGCAACGGTCAAACGTCAANGTTTTGTACACAGGAAAGTCTTTAAGACTTAACCGTACTTTTCATGATAACGGAGANGTTTTGCCTAGTCCGTCGTCTTCATGANCTTTGATTTANTAAAGAAAAAAGATTTGTTCTCTTTTAATNNNTNTTAAGTATTTGTTTNTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNATNNNNNTTNNNTTTTTCTAAGANTANGATATNCATTANNGGNGNGNNNNATTATNTATTTATTTNTTTGTTANNATNNCTTTGTCGCCATTGGTGNNNATNAAANATTAAAANGTTGTNNAATTTAGANTTTNGCGTTTGTTNTANCAAATGTGNTTTTTNNANAAAANANNGTAAANANAATAANATGAAATAANNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTNNTANNGNNNAAAANAGAAAATAANANAATNNATNNGTTTAAANCNTAAATNTNAATTAAAATGNNACTGATNNATTATNTTCNNANATACATNGGAACNCTTNAANNTATGAACTGNAAAACTNTNTTTTNTTNNAGNGNNACTAAATGACCANATTTTNNTAAANCGACANATGGAAGACTAGTATNTTNTAACCAACAATNNACAATAACCAATANTGNNNNNNNNTATTGTTTNTTTGATNNTAGAATTGTTACATCCNGTANGACNAAGTNTGANNNATNATAANNNNNNNNNNNNNNNNNNGAGTCNNNTCACACNATNGNTNGCANTGNGNTTCNAACATTTATCCACTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNTTTNNTGCTNTAATGANTGNAACNATNCANNNNNNGTNNTCTAAATAGAATCGAACTAAAAGAAAAATTNNACNTAAAAAANCAAATTGCNATATNTTTTGNTTNTNTTTGGTTTAAANCTATNTATGATCTANANAAAANANATTATGAGCNNAAAGTGAATTANTCTNATNNTATATNNNNNCAAACGGTAAGTATTANGTTAGAAAACTNAATAGTCTTATGTATANAAATCTCTTCATAATNCANNAAATATATTNTANATTNTAATGANTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_5_21480023_21480087_zu-0_hi-0_624310_25927_TRUE
SEQUENCE_TARGET=950,164
SEQUENCE_TEMPLATE=CGAGATCGCCAAATCAATTTTCTCTGGAAAATTCGAAAATGTCGAAGCCGCATGTTGCAAGGCGTTTTCAACCTTAGATGCTAACTGTTGGCCCCATATGTTTCCGTTGAATCCATTTTTCCCTCCTCTCCTCAAGGACAACTGCGCTCGCATTGTCCCCAACTCCCCTACACACAACTAAGANCAAGAATTNTTGCTGGTCTACGCTTTTAAATTAGTCATTNGNTCCAAAGTCTAATAAGTTTCAATGTTTATTTTGTTNGCAAAATGTGAANTATATCTTGGAAGAGTTCTCTTTTTATAGNTTTTCTCAATGACATAAAATTTCAATATTTTCCTTTTATATAATAACTCTTATAAATGTGTTTTGTTGGTTGCACAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAANATTGGAAAATCGTACCTCAAAATNCAANNACCAAACAAACCTTCNNNNNTGNATCCTATAGTATGGTTATTTCCGGTNTAGTTGGTTCGGTTCTGNNCCGAAATTATATCGAGCGTTTTAACGTTATCCAGAACGAAGCAGAGTAACGAAGAGTCATGGCGATGCTTTTTCGACCTCCTCCTTCACAATGTAGAAGCTTCTCTCCTTTTGTCTTCAACTNCAGCTCCAGAGAAGTTTCTTCNTCGTCTCGTNTCTCCTTGAAAANCTCCGGCGACGAAGAAAACTGGGTTTCACGTTTCCGGTCGAAATCTCTCAGTTTGGTGTTCTCTGGAGCTCTTGCTNTCGGTTTATCTCTATCAGGTACGTTTCNTTGTTCNTTTTNACCAAGATCATTTCTTTTTGNCTNAANATTGAACAACATGCTCTTTGATTTCANTAACAATGTGGATGTNTTGCAAAACNNTCTAAAANATCNCAAAGNTAGAATNTTTGTTTACTCNNNAATNTCAAGCTTGTGACTTTAATTACTTNNTGCATTGGCTTATCAAAGTCAGNANTNTGAAANTTCGNACNTANTCTCTTNTTCNNTAACTATGAANAATGCANATGNANTTTCNAGATTNNANNTNTNAATAACTNTGAATCTNNTTTTGNAGGCGTGGGGTTTGCAGANGCAAAAGTTGGTGTTAACAAACCAGAANTGCTTCCTAAAGAGTTNACTTCTGTTATAGATGTTGCTGATTTCCTCTCTAATGGTCAGGTATGCATGNTTGTANCATCCATCTANTTTTGAGGTTTGATTAAAGCACTTGTTTCTTGTTAATGTCATGTGGATCTATTNGCTTATTCTGTAGATTTTTNGTGTATTTCTGATTTTGAATGTTTTCGCCGCTTTTGGTTTTCTTTTCTNAATGATGTTGTTCTTTGAGAGTTATAGGAGAAGAGNATTGCTCAAGAAATCGCTAATCTTGAGAAAGATACAGGATTCAAGTTGAGAGTCTTGGCTCAAAACTATCCCGTTACNCCTGGTATGATATATGCTTNGAAAACAACATACTTGATCTCTATANAAGTTTACTTAGNGNCATTTCTCTGTGTTTGNAACAGGACTAGCAATCAAAGATTTCTGGCAGGTGGATGATAGCACAATTGTGTTTGTCGCTGACCCGACTTTTGGTAACANCCCAAACCTCATTNCTTAAGTTCTTGAAGTANCCNNATNGGAAACTGATTGTGATNANCGGATTTGANACAGGGAACATATTGAATTTCAANGTTGGAGCNACGNTTGATTTAGACATACCTCGTAGTTTCTGGAGTCGACTGGCTGGGAAATACGGCAANATGTTTTACTGGAAAGAGAAGGCAAGAAAGCTACCTCGATTNAAAAAACCGATGNTCGCCACTTTCTTTGTTTTTTGTTNGNATGTCTAAACTGTTTACAATATGNCTTGAAACCAGNGAGAAGATGCATCNATTNAAGCTGNAGTGATGGCNATATCANGNTGCTTNCGAGAACCAGTTGGTCNAAANAACTGTGCAGAGATCCAATGAAGTATATGACCCCATNAAGCGTATATGTTATNNNNNNNNTATCANTGTATANATTGGCANCAAAGANA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.609076
PRIMER_LEFT_0_PENALTY=0.173354
PRIMER_RIGHT_0_PENALTY=0.435722
PRIMER_LEFT_0_SEQUENCE=GCTTTTTCGACCTCCTCCTT
PRIMER_RIGHT_0_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_0=605,20
PRIMER_RIGHT_0=1332,20
PRIMER_LEFT_0_TM=59.827
PRIMER_RIGHT_0_TM=60.436
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=728
PRIMER_PAIR_1_PENALTY=0.632625
PRIMER_LEFT_1_PENALTY=0.196903
PRIMER_RIGHT_1_PENALTY=0.435722
PRIMER_LEFT_1_SEQUENCE=TCCGGTCGAAATCTCTCAGT
PRIMER_RIGHT_1_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_1=739,20
PRIMER_RIGHT_1=1332,20
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=60.436
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=594
PRIMER_PAIR_2_PENALTY=0.651624
PRIMER_LEFT_2_PENALTY=0.215902
PRIMER_RIGHT_2_PENALTY=0.435722
PRIMER_LEFT_2_SEQUENCE=TTTTTCGACCTCCTCCTTCA
PRIMER_RIGHT_2_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_2=607,20
PRIMER_RIGHT_2=1332,20
PRIMER_LEFT_2_TM=59.784
PRIMER_RIGHT_2_TM=60.436
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=726
PRIMER_PAIR_3_PENALTY=0.657089
PRIMER_LEFT_3_PENALTY=0.221368
PRIMER_RIGHT_3_PENALTY=0.435722
PRIMER_LEFT_3_SEQUENCE=AGTCATGGCGATGCTTTTTC
PRIMER_RIGHT_3_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_3=593,20
PRIMER_RIGHT_3=1332,20
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=60.436
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=740
PRIMER_PAIR_4_PENALTY=0.657089
PRIMER_LEFT_4_PENALTY=0.221368
PRIMER_RIGHT_4_PENALTY=0.435722
PRIMER_LEFT_4_SEQUENCE=GAGTCATGGCGATGCTTTTT
PRIMER_RIGHT_4_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_4=592,20
PRIMER_RIGHT_4=1332,20
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=60.436
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=741
PRIMER_PAIR_5_PENALTY=1.014090
PRIMER_LEFT_5_PENALTY=0.578368
PRIMER_RIGHT_5_PENALTY=0.435722
PRIMER_LEFT_5_SEQUENCE=ATGCTTTTTCGACCTCCTCC
PRIMER_RIGHT_5_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_5=603,20
PRIMER_RIGHT_5=1332,20
PRIMER_LEFT_5_TM=60.578
PRIMER_RIGHT_5_TM=60.436
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=730
PRIMER_PAIR_6_PENALTY=1.098109
PRIMER_LEFT_6_PENALTY=0.173354
PRIMER_RIGHT_6_PENALTY=0.924755
PRIMER_LEFT_6_SEQUENCE=GCTTTTTCGACCTCCTCCTT
PRIMER_RIGHT_6_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_6=605,20
PRIMER_RIGHT_6=1584,20
PRIMER_LEFT_6_TM=59.827
PRIMER_RIGHT_6_TM=59.075
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=980
PRIMER_PAIR_7_PENALTY=1.121658
PRIMER_LEFT_7_PENALTY=0.196903
PRIMER_RIGHT_7_PENALTY=0.924755
PRIMER_LEFT_7_SEQUENCE=TCCGGTCGAAATCTCTCAGT
PRIMER_RIGHT_7_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_7=739,20
PRIMER_RIGHT_7=1584,20
PRIMER_LEFT_7_TM=59.803
PRIMER_RIGHT_7_TM=59.075
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=846
PRIMER_PAIR_8_PENALTY=1.140657
PRIMER_LEFT_8_PENALTY=0.215902
PRIMER_RIGHT_8_PENALTY=0.924755
PRIMER_LEFT_8_SEQUENCE=TTTTTCGACCTCCTCCTTCA
PRIMER_RIGHT_8_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_8=607,20
PRIMER_RIGHT_8=1584,20
PRIMER_LEFT_8_TM=59.784
PRIMER_RIGHT_8_TM=59.075
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=978
PRIMER_PAIR_9_PENALTY=1.146123
PRIMER_LEFT_9_PENALTY=0.221368
PRIMER_RIGHT_9_PENALTY=0.924755
PRIMER_LEFT_9_SEQUENCE=GAGTCATGGCGATGCTTTTT
PRIMER_RIGHT_9_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_9=592,20
PRIMER_RIGHT_9=1584,20
PRIMER_LEFT_9_TM=60.221
PRIMER_RIGHT_9_TM=59.075
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=993
PRIMER_PAIR_10_PENALTY=1.148949
PRIMER_LEFT_10_PENALTY=0.713227
PRIMER_RIGHT_10_PENALTY=0.435722
PRIMER_LEFT_10_SEQUENCE=TTTCCGGTCGAAATCTCTCA
PRIMER_RIGHT_10_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_10=737,20
PRIMER_RIGHT_10=1332,20
PRIMER_LEFT_10_TM=60.713
PRIMER_RIGHT_10_TM=60.436
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=596
PRIMER_PAIR_11_PENALTY=1.151848
PRIMER_LEFT_11_PENALTY=0.716126
PRIMER_RIGHT_11_PENALTY=0.435722
PRIMER_LEFT_11_SEQUENCE=CCGGTCGAAATCTCTCAGTT
PRIMER_RIGHT_11_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_11=740,20
PRIMER_RIGHT_11=1332,20
PRIMER_LEFT_11_TM=59.284
PRIMER_RIGHT_11_TM=60.436
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=593
PRIMER_PAIR_12_PENALTY=1.176537
PRIMER_LEFT_12_PENALTY=0.740815
PRIMER_RIGHT_12_PENALTY=0.435722
PRIMER_LEFT_12_SEQUENCE=TTTTCGACCTCCTCCTTCAC
PRIMER_RIGHT_12_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_12=608,20
PRIMER_RIGHT_12=1332,20
PRIMER_LEFT_12_TM=59.259
PRIMER_RIGHT_12_TM=60.436
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=725
PRIMER_PAIR_13_PENALTY=1.204939
PRIMER_LEFT_13_PENALTY=0.769217
PRIMER_RIGHT_13_PENALTY=0.435722
PRIMER_LEFT_13_SEQUENCE=TTCGACCTCCTCCTTCACAA
PRIMER_RIGHT_13_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_13=610,20
PRIMER_RIGHT_13=1332,20
PRIMER_LEFT_13_TM=60.769
PRIMER_RIGHT_13_TM=60.436
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=723
PRIMER_PAIR_14_PENALTY=1.204939
PRIMER_LEFT_14_PENALTY=0.769217
PRIMER_RIGHT_14_PENALTY=0.435722
PRIMER_LEFT_14_SEQUENCE=TTTCGACCTCCTCCTTCACA
PRIMER_RIGHT_14_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_14=609,20
PRIMER_RIGHT_14=1332,20
PRIMER_LEFT_14_TM=60.769
PRIMER_RIGHT_14_TM=60.436
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=724
PRIMER_PAIR_15_PENALTY=1.212689
PRIMER_LEFT_15_PENALTY=0.196903
PRIMER_RIGHT_15_PENALTY=1.015786
PRIMER_LEFT_15_SEQUENCE=TCCGGTCGAAATCTCTCAGT
PRIMER_RIGHT_15_SEQUENCE=TGTTACCAAAAGTCGGGTCAG
PRIMER_LEFT_15=739,20
PRIMER_RIGHT_15=1623,21
PRIMER_LEFT_15_TM=59.803
PRIMER_RIGHT_15_TM=60.016
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=885
PRIMER_PAIR_16_PENALTY=1.254391
PRIMER_LEFT_16_PENALTY=0.173354
PRIMER_RIGHT_16_PENALTY=1.081037
PRIMER_LEFT_16_SEQUENCE=GCTTTTTCGACCTCCTCCTT
PRIMER_RIGHT_16_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_16=605,20
PRIMER_RIGHT_16=1585,21
PRIMER_LEFT_16_TM=59.827
PRIMER_RIGHT_16_TM=60.081
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=981
PRIMER_PAIR_17_PENALTY=1.277940
PRIMER_LEFT_17_PENALTY=0.196903
PRIMER_RIGHT_17_PENALTY=1.081037
PRIMER_LEFT_17_SEQUENCE=TCCGGTCGAAATCTCTCAGT
PRIMER_RIGHT_17_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_17=739,20
PRIMER_RIGHT_17=1585,21
PRIMER_LEFT_17_TM=59.803
PRIMER_RIGHT_17_TM=60.081
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=847
PRIMER_PAIR_18_PENALTY=1.296939
PRIMER_LEFT_18_PENALTY=0.215902
PRIMER_RIGHT_18_PENALTY=1.081037
PRIMER_LEFT_18_SEQUENCE=TTTTTCGACCTCCTCCTTCA
PRIMER_RIGHT_18_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_18=607,20
PRIMER_RIGHT_18=1585,21
PRIMER_LEFT_18_TM=59.784
PRIMER_RIGHT_18_TM=60.081
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=979
PRIMER_PAIR_19_PENALTY=1.302405
PRIMER_LEFT_19_PENALTY=0.221368
PRIMER_RIGHT_19_PENALTY=1.081037
PRIMER_LEFT_19_SEQUENCE=GAGTCATGGCGATGCTTTTT
PRIMER_RIGHT_19_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_19=592,20
PRIMER_RIGHT_19=1585,21
PRIMER_LEFT_19_TM=60.221
PRIMER_RIGHT_19_TM=60.081
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=994
PRIMER_PAIR_20_PENALTY=1.321576
PRIMER_LEFT_20_PENALTY=0.173354
PRIMER_RIGHT_20_PENALTY=1.148222
PRIMER_LEFT_20_SEQUENCE=GCTTTTTCGACCTCCTCCTT
PRIMER_RIGHT_20_SEQUENCE=GAAAAGAAAACCAAAAGCGG
PRIMER_LEFT_20=605,20
PRIMER_RIGHT_20=1349,20
PRIMER_LEFT_20_TM=59.827
PRIMER_RIGHT_20_TM=58.852
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=745
PRIMER_PAIR_21_PENALTY=1.345125
PRIMER_LEFT_21_PENALTY=0.196903
PRIMER_RIGHT_21_PENALTY=1.148222
PRIMER_LEFT_21_SEQUENCE=TCCGGTCGAAATCTCTCAGT
PRIMER_RIGHT_21_SEQUENCE=GAAAAGAAAACCAAAAGCGG
PRIMER_LEFT_21=739,20
PRIMER_RIGHT_21=1349,20
PRIMER_LEFT_21_TM=59.803
PRIMER_RIGHT_21_TM=58.852
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=11.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=611
PRIMER_PAIR_22_PENALTY=1.364125
PRIMER_LEFT_22_PENALTY=0.215902
PRIMER_RIGHT_22_PENALTY=1.148222
PRIMER_LEFT_22_SEQUENCE=TTTTTCGACCTCCTCCTTCA
PRIMER_RIGHT_22_SEQUENCE=GAAAAGAAAACCAAAAGCGG
PRIMER_LEFT_22=607,20
PRIMER_RIGHT_22=1349,20
PRIMER_LEFT_22_TM=59.784
PRIMER_RIGHT_22_TM=58.852
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=11.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=743
PRIMER_PAIR_23_PENALTY=1.369590
PRIMER_LEFT_23_PENALTY=0.221368
PRIMER_RIGHT_23_PENALTY=1.148222
PRIMER_LEFT_23_SEQUENCE=AGTCATGGCGATGCTTTTTC
PRIMER_RIGHT_23_SEQUENCE=GAAAAGAAAACCAAAAGCGG
PRIMER_LEFT_23=593,20
PRIMER_RIGHT_23=1349,20
PRIMER_LEFT_23_TM=60.221
PRIMER_RIGHT_23_TM=58.852
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=11.4000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=757
PRIMER_PAIR_24_PENALTY=1.369590
PRIMER_LEFT_24_PENALTY=0.221368
PRIMER_RIGHT_24_PENALTY=1.148222
PRIMER_LEFT_24_SEQUENCE=GAGTCATGGCGATGCTTTTT
PRIMER_RIGHT_24_SEQUENCE=GAAAAGAAAACCAAAAGCGG
PRIMER_LEFT_24=592,20
PRIMER_RIGHT_24=1349,20
PRIMER_LEFT_24_TM=60.221
PRIMER_RIGHT_24_TM=58.852
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=11.4000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=758
PRIMER_PAIR_25_PENALTY=1.502087
PRIMER_LEFT_25_PENALTY=1.066365
PRIMER_RIGHT_25_PENALTY=0.435722
PRIMER_LEFT_25_SEQUENCE=AAACTGGGTTTCACGTTTCC
PRIMER_RIGHT_25_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_25=722,20
PRIMER_RIGHT_25=1332,20
PRIMER_LEFT_25_TM=58.934
PRIMER_RIGHT_25_TM=60.436
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=611
PRIMER_PAIR_26_PENALTY=1.503123
PRIMER_LEFT_26_PENALTY=0.578368
PRIMER_RIGHT_26_PENALTY=0.924755
PRIMER_LEFT_26_SEQUENCE=ATGCTTTTTCGACCTCCTCC
PRIMER_RIGHT_26_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_26=603,20
PRIMER_RIGHT_26=1584,20
PRIMER_LEFT_26_TM=60.578
PRIMER_RIGHT_26_TM=59.075
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=982
PRIMER_PAIR_27_PENALTY=1.503388
PRIMER_LEFT_27_PENALTY=0.173354
PRIMER_RIGHT_27_PENALTY=1.330034
PRIMER_LEFT_27_SEQUENCE=GCTTTTTCGACCTCCTCCTT
PRIMER_RIGHT_27_SEQUENCE=CTATCATCCACCTGCCAGAA
PRIMER_LEFT_27=605,20
PRIMER_RIGHT_27=1585,20
PRIMER_LEFT_27_TM=59.827
PRIMER_RIGHT_27_TM=58.670
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=981
PRIMER_PAIR_28_PENALTY=1.526937
PRIMER_LEFT_28_PENALTY=0.196903
PRIMER_RIGHT_28_PENALTY=1.330034
PRIMER_LEFT_28_SEQUENCE=TCCGGTCGAAATCTCTCAGT
PRIMER_RIGHT_28_SEQUENCE=CTATCATCCACCTGCCAGAA
PRIMER_LEFT_28=739,20
PRIMER_RIGHT_28=1585,20
PRIMER_LEFT_28_TM=59.803
PRIMER_RIGHT_28_TM=58.670
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=847
PRIMER_PAIR_29_PENALTY=1.545936
PRIMER_LEFT_29_PENALTY=0.215902
PRIMER_RIGHT_29_PENALTY=1.330034
PRIMER_LEFT_29_SEQUENCE=TTTTTCGACCTCCTCCTTCA
PRIMER_RIGHT_29_SEQUENCE=CTATCATCCACCTGCCAGAA
PRIMER_LEFT_29=607,20
PRIMER_RIGHT_29=1585,20
PRIMER_LEFT_29_TM=59.784
PRIMER_RIGHT_29_TM=58.670
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=979
PRIMER_PAIR_30_PENALTY=1.551401
PRIMER_LEFT_30_PENALTY=0.221368
PRIMER_RIGHT_30_PENALTY=1.330034
PRIMER_LEFT_30_SEQUENCE=AGTCATGGCGATGCTTTTTC
PRIMER_RIGHT_30_SEQUENCE=CTATCATCCACCTGCCAGAA
PRIMER_LEFT_30=593,20
PRIMER_RIGHT_30=1585,20
PRIMER_LEFT_30_TM=60.221
PRIMER_RIGHT_30_TM=58.670
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=993
PRIMER_PAIR_31_PENALTY=1.551401
PRIMER_LEFT_31_PENALTY=0.221368
PRIMER_RIGHT_31_PENALTY=1.330034
PRIMER_LEFT_31_SEQUENCE=GAGTCATGGCGATGCTTTTT
PRIMER_RIGHT_31_SEQUENCE=CTATCATCCACCTGCCAGAA
PRIMER_LEFT_31=592,20
PRIMER_RIGHT_31=1585,20
PRIMER_LEFT_31_TM=60.221
PRIMER_RIGHT_31_TM=58.670
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=994
PRIMER_PAIR_32_PENALTY=1.571887
PRIMER_LEFT_32_PENALTY=1.136165
PRIMER_RIGHT_32_PENALTY=0.435722
PRIMER_LEFT_32_SEQUENCE=GATGCTTTTTCGACCTCCTC
PRIMER_RIGHT_32_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_32=602,20
PRIMER_RIGHT_32=1332,20
PRIMER_LEFT_32_TM=58.864
PRIMER_RIGHT_32_TM=60.436
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=731
PRIMER_PAIR_33_PENALTY=1.574914
PRIMER_LEFT_33_PENALTY=1.139192
PRIMER_RIGHT_33_PENALTY=0.435722
PRIMER_LEFT_33_SEQUENCE=CGGTCGAAATCTCTCAGTTTG
PRIMER_RIGHT_33_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_33=741,21
PRIMER_RIGHT_33=1332,20
PRIMER_LEFT_33_TM=59.861
PRIMER_RIGHT_33_TM=60.436
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=592
PRIMER_PAIR_34_PENALTY=1.637982
PRIMER_LEFT_34_PENALTY=0.713227
PRIMER_RIGHT_34_PENALTY=0.924755
PRIMER_LEFT_34_SEQUENCE=TTTCCGGTCGAAATCTCTCA
PRIMER_RIGHT_34_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_34=737,20
PRIMER_RIGHT_34=1584,20
PRIMER_LEFT_34_TM=60.713
PRIMER_RIGHT_34_TM=59.075
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=848
PRIMER_PAIR_35_PENALTY=1.640881
PRIMER_LEFT_35_PENALTY=0.716126
PRIMER_RIGHT_35_PENALTY=0.924755
PRIMER_LEFT_35_SEQUENCE=CCGGTCGAAATCTCTCAGTT
PRIMER_RIGHT_35_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_35=740,20
PRIMER_RIGHT_35=1584,20
PRIMER_LEFT_35_TM=59.284
PRIMER_RIGHT_35_TM=59.075
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=845
PRIMER_PAIR_36_PENALTY=1.659405
PRIMER_LEFT_36_PENALTY=0.578368
PRIMER_RIGHT_36_PENALTY=1.081037
PRIMER_LEFT_36_SEQUENCE=ATGCTTTTTCGACCTCCTCC
PRIMER_RIGHT_36_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_36=603,20
PRIMER_RIGHT_36=1585,21
PRIMER_LEFT_36_TM=60.578
PRIMER_RIGHT_36_TM=60.081
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=983
PRIMER_PAIR_37_PENALTY=1.665570
PRIMER_LEFT_37_PENALTY=0.740815
PRIMER_RIGHT_37_PENALTY=0.924755
PRIMER_LEFT_37_SEQUENCE=TTTTCGACCTCCTCCTTCAC
PRIMER_RIGHT_37_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_37=608,20
PRIMER_RIGHT_37=1584,20
PRIMER_LEFT_37_TM=59.259
PRIMER_RIGHT_37_TM=59.075
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=977
PRIMER_PAIR_38_PENALTY=1.675174
PRIMER_LEFT_38_PENALTY=1.239452
PRIMER_RIGHT_38_PENALTY=0.435722
PRIMER_LEFT_38_SEQUENCE=AACGTTATCCAGAACGAAGCA
PRIMER_RIGHT_38_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_38=561,21
PRIMER_RIGHT_38=1332,20
PRIMER_LEFT_38_TM=59.761
PRIMER_RIGHT_38_TM=60.436
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=772
PRIMER_PAIR_39_PENALTY=1.683746
PRIMER_LEFT_39_PENALTY=1.248025
PRIMER_RIGHT_39_PENALTY=0.435722
PRIMER_LEFT_39_SEQUENCE=AAAACTGGGTTTCACGTTTCC
PRIMER_RIGHT_39_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_39=721,21
PRIMER_RIGHT_39=1332,20
PRIMER_LEFT_39_TM=60.248
PRIMER_RIGHT_39_TM=60.436
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=612
PRIMER_PAIR_40_PENALTY=1.692159
PRIMER_LEFT_40_PENALTY=1.256437
PRIMER_RIGHT_40_PENALTY=0.435722
PRIMER_LEFT_40_SEQUENCE=TGCTTTTTCGACCTCCTCCT
PRIMER_RIGHT_40_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_40=604,20
PRIMER_RIGHT_40=1332,20
PRIMER_LEFT_40_TM=61.256
PRIMER_RIGHT_40_TM=60.436
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=729
PRIMER_PAIR_41_PENALTY=1.693972
PRIMER_LEFT_41_PENALTY=0.769217
PRIMER_RIGHT_41_PENALTY=0.924755
PRIMER_LEFT_41_SEQUENCE=TTCGACCTCCTCCTTCACAA
PRIMER_RIGHT_41_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_41=610,20
PRIMER_RIGHT_41=1584,20
PRIMER_LEFT_41_TM=60.769
PRIMER_RIGHT_41_TM=59.075
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=975
PRIMER_PAIR_42_PENALTY=1.693972
PRIMER_LEFT_42_PENALTY=0.769217
PRIMER_RIGHT_42_PENALTY=0.924755
PRIMER_LEFT_42_SEQUENCE=TTTCGACCTCCTCCTTCACA
PRIMER_RIGHT_42_SEQUENCE=TATCATCCACCTGCCAGAAA
PRIMER_LEFT_42=609,20
PRIMER_RIGHT_42=1584,20
PRIMER_LEFT_42_TM=60.769
PRIMER_RIGHT_42_TM=59.075
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=976
PRIMER_PAIR_43_PENALTY=1.726591
PRIMER_LEFT_43_PENALTY=0.578368
PRIMER_RIGHT_43_PENALTY=1.148222
PRIMER_LEFT_43_SEQUENCE=ATGCTTTTTCGACCTCCTCC
PRIMER_RIGHT_43_SEQUENCE=GAAAAGAAAACCAAAAGCGG
PRIMER_LEFT_43=603,20
PRIMER_RIGHT_43=1349,20
PRIMER_LEFT_43_TM=60.578
PRIMER_RIGHT_43_TM=58.852
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=11.4000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=747
PRIMER_PAIR_44_PENALTY=1.729013
PRIMER_LEFT_44_PENALTY=0.713227
PRIMER_RIGHT_44_PENALTY=1.015786
PRIMER_LEFT_44_SEQUENCE=TTTCCGGTCGAAATCTCTCA
PRIMER_RIGHT_44_SEQUENCE=TGTTACCAAAAGTCGGGTCAG
PRIMER_LEFT_44=737,20
PRIMER_RIGHT_44=1623,21
PRIMER_LEFT_44_TM=60.713
PRIMER_RIGHT_44_TM=60.016
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=887
PRIMER_PAIR_45_PENALTY=1.731912
PRIMER_LEFT_45_PENALTY=0.716126
PRIMER_RIGHT_45_PENALTY=1.015786
PRIMER_LEFT_45_SEQUENCE=CCGGTCGAAATCTCTCAGTT
PRIMER_RIGHT_45_SEQUENCE=TGTTACCAAAAGTCGGGTCAG
PRIMER_LEFT_45=740,20
PRIMER_RIGHT_45=1623,21
PRIMER_LEFT_45_TM=59.284
PRIMER_RIGHT_45_TM=60.016
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=884
PRIMER_PAIR_46_PENALTY=1.794264
PRIMER_LEFT_46_PENALTY=0.713227
PRIMER_RIGHT_46_PENALTY=1.081037
PRIMER_LEFT_46_SEQUENCE=TTTCCGGTCGAAATCTCTCA
PRIMER_RIGHT_46_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_46=737,20
PRIMER_RIGHT_46=1585,21
PRIMER_LEFT_46_TM=60.713
PRIMER_RIGHT_46_TM=60.081
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=849
PRIMER_PAIR_47_PENALTY=1.797163
PRIMER_LEFT_47_PENALTY=0.716126
PRIMER_RIGHT_47_PENALTY=1.081037
PRIMER_LEFT_47_SEQUENCE=CCGGTCGAAATCTCTCAGTT
PRIMER_RIGHT_47_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_47=740,20
PRIMER_RIGHT_47=1585,21
PRIMER_LEFT_47_TM=59.284
PRIMER_RIGHT_47_TM=60.081
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=846
PRIMER_PAIR_48_PENALTY=1.821852
PRIMER_LEFT_48_PENALTY=0.740815
PRIMER_RIGHT_48_PENALTY=1.081037
PRIMER_LEFT_48_SEQUENCE=TTTTCGACCTCCTCCTTCAC
PRIMER_RIGHT_48_SEQUENCE=CTATCATCCACCTGCCAGAAA
PRIMER_LEFT_48=608,20
PRIMER_RIGHT_48=1585,21
PRIMER_LEFT_48_TM=59.259
PRIMER_RIGHT_48_TM=60.081
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=978
PRIMER_PAIR_49_PENALTY=1.828323
PRIMER_LEFT_49_PENALTY=1.392601
PRIMER_RIGHT_49_PENALTY=0.435722
PRIMER_LEFT_49_SEQUENCE=CGTTATCCAGAACGAAGCAGA
PRIMER_RIGHT_49_SEQUENCE=CGGCGAAAACATTCAAAATC
PRIMER_LEFT_49=563,21
PRIMER_RIGHT_49=1332,20
PRIMER_LEFT_49_TM=60.393
PRIMER_RIGHT_49_TM=60.436
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=770
=
SEQUENCE_ID=MAGIC.338_3_13154001_13154021_no-0_tsu-0_1460707_5503_TRUE
SEQUENCE_TARGET=950,120
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNAAAAAGTAAGCANATATANNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_1317190_1317217_tsu-0_hi-0_3755_222380_TRUE
SEQUENCE_TARGET=950,127
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTANTGACAAANCAGAGCAAAAGACAGTAAAGTTTCTTGCTTTATTCAAAAACTAAAACCAAAGAAAAATCACATTACTAATTTGCTTATAAACACTCTGCGACTAATAACCGTTGGATCATATCAGTAAGGAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATCACGAAATTAGCAAACTCTCGTCACAAATCATCAGTTTCTCTTTCTCTCTTCCTCNTTAGTCTCTCTCCACTGATCTTGAAACTACAACNATGAACGTGTACATCGATACTGAGACTGGCTCATCGTTCTCCATTACTATTGATTTCGGCGAAACAGTGTTANAGATNAAAGAGAAGATCGAGAAGTCTCAAGGTATCCCTGTTTCGAAACAAATCCTTTACTTGGATGNCAAGGCTCTTGAANNNGATCTTCATAAGATTGATTACATGATCCTCTTCGAATCTCGTCTCCTTCTCCGCATCTCTCCTGATGCTNACCCTAATCAGAGCAACGAGCAAACCGAGCAATCAAAACAAATTGATGATAAGAAACAAGAGTTCTGTGGCATTCAAGATTCATCGGAGAGCAAAAAGNTCACGAGGGTGATGGCGAGGAGAGTCCACAATATATATTCATCACTACCGGCTTATTCACTTGATGAGTTACTTGGCCCTAAATATTCAGCAACTGTGNCGGTTGGAGGCAGAACAAATCAAGTGGTTCAANCCACCGAGCAAGCTTCAACGTCAGGCACAGCCAAAGAGGTCCTTAGAGATTCAGATTCGCCTGTGGANAAGAAGATCAAGACCAACCCGATGAAGTTTACAGTACATGTGAAGCCATATCAAGAAGATACCANGATGATTCANGTAGAGGTTAACGCAGATGATAACGTCGAAGAACTGAGGAAAGAGCTGGTTAAGATGCAAGAGAGGGGTGAGTTAAATCTNCCTCACGAAGCGTTTCACTTGGTAANCAGTGAGTTAC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17514238_17514550_can-0_rsch-4_3957_2384_TRUE
SEQUENCE_TARGET=950,412
SEQUENCE_TEMPLATE=TATAGGTTCTGGTGGTAAAGACCAAGGAGAGGATGGTGAAGATTGNCAAGATGATGACCTATCTGATGCTGATGNTGATGCTGATGAAGATGAGTCTGATTTGGATATGGCNTGGAAAATGCTGGACATTGCTAGGGTTATTACTGACAAACAGTCTACTGAGACNATGGAAAAAGTGGATATACTCTGTTCGCTTGCTGAAGTNTCCTTGGAAAGAGGTATTTTTNNTTCCTTTCACCTTTGCATTGTCTTATTTTCTCATNTCAGCTATTGGTTGGTNTTGAGTTCTGTTGTTTACTNTGCAGAGGACATTGAGTCTTCCCTGNCTGACTACAAGAATGCATTATCCATCTTAGANNGACTGGTTGAGCCCGACAGTCGACGCACAGCTGAATTGTATCCTTNTGNTTTCTTAAATNGTANCTGATTTTCGTTTCTGTCAATTNAGTTGTTGGATTATANCTTAACTTGANCGTTTCAGAAACTTCCGCATCTGCATTTGTCTTGAGACTGGATGTCAGCCTAAGGAAGCAATACCATATTGTCAAANAGCTTTGTTAATATGCAAAGCTCGGATGGAGAGNCTCAGTAATGAAATCAAAGGTGCATCTGGTTCAGCAACTTCCTCAACCGTCTCTGAAATAGATGAAGGCATCCAACAATCATCCAATGTTCCTTACATTGATAAANCTGCTTCAGACAAAGAAGTNGAGATNGGAGACTTAGCTGGTCTCGCTGAAGATCTAGAAAAGAAGGCAAGTAAACTAAACCTATCAGTTCATTAAAATANCNCTAAACCCATACTAATGTCCAACATTTNTTTTCTGNAGCTTGAGGATTTNAAACAACAAGCAGAGAACCCAAAGCANGTTCTTGCTGAGCTAATGGGCATGGTATCAGCTAAGCCAAATGCCAGTGACAAGGTTGTTCCTGCTGCTGCTGAAATGAGCTCTTCNAGGATGGGAACTGTGAANACCAATTTCGGNAAAGATTTAGAGTCNCCTACAGTCTCAACCGCTCATACCGGTGCAGCAGGAGGAGGAGCAGCATCAGGTGTTACTCACTTGGGTGTTGTTGGAAGAGGAGTGAAGAGAGTTTTGATGAATACAACCTCGATTGAATCAAGTGCATCAAAGAAACCAGCTCTAGAGTTNTCAGACAAAGCAGATGGCAACTCCTCTTGAAACCGGAAAAAGGTATAAAAATTCCTGTTACTAGTGAAGTATCTAACCTAAGAAAACTGAGAAAAACATTTGGTATCTTCCTCATGAATCNTCCAAGACTAGATTTTCAGGTTCATGTTTTGTAATGGNGTCCGTACTTNCTACTTTTTTNAATCGCTGAGATNTTTTTTACTTTTTATTNTTGTTTAATCAGTCTTATTTTTTNAANGTGNTTTTTAGTTTAGGCACAAAAGCAGAATCTTATTATCACATTATCAGTTGATAATCATCTGATGAGAAATAGTTCTTTGGAGATCTTTTAGGGAATTTTATAANTAATAATTAAGGNATAANNGGATATCCTTTATTAAAAAATGTAGGNTACTTGTAGAATTAAAAACTGGAANNTTNTAATATGANTAGTATAATAAGATTACGCTATATCTGGATTCTGGAGATATCATAGATAAGCTCTGTCTATGAAAACGAGACCCTTGGNGAAGCCACGTGTNAAATCTATNTCTGTNACCAGCGAAGCCTACGTGGCGAGCAGCTCCTAGAANATCNAAATTNACAACAGTANTTTTTTTTCTTTCGTTCTGATTCAACAGTTGTCACAANCAGCNAAGGTGTTNTGTGATTGGTTATAAATACTCTGNTATGTGAGAGANTNTACTACATTCACAAANAAACTAAAACTTAAAAATGGGNAGGGGTGAGTTTTTGGCCATGAAGACTGAGGAGAACGCGGCTAACCTGATCAATTCCGATATGAATGAGTTCGTCGCCGCTGCAAAGAAGCTCGTGAAAGACGTAGGGATGCTCGGCGGGGTAGGTTTTGGCACGTCAGTCCTCCAATGGGCCGCTTCAATCTTCGCTATGTGAGCCAAAAGACAAAACCATATAACCTCTCTGGNTTTTGTTTGAATCATATATTGATATGATGGTTCTTATTGTTTTGCTTTGACAGTTATCTGTTGATATTGGATCGTACCAACTGGAAAACCAAAATGTTGACCACCCTTTTAGTACCATACATCTTTTTCACACTTCCTTCGGTTATCTTCCAGTTCTTTAGGTAATCAAGAAAAACTNTTCTTTAACATGATGTNATGGAGTTCTTTTGANAATNTAATCNATATTCGATAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.839004
PRIMER_LEFT_0_PENALTY=0.085951
PRIMER_RIGHT_0_PENALTY=0.753052
PRIMER_LEFT_0_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_0_SEQUENCE=TCTTCATGGCCAAAAACTCA
PRIMER_LEFT_0=918,20
PRIMER_RIGHT_0=1898,20
PRIMER_LEFT_0_TM=59.914
PRIMER_RIGHT_0_TM=59.247
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=981
PRIMER_PAIR_1_PENALTY=0.959521
PRIMER_LEFT_1_PENALTY=0.085951
PRIMER_RIGHT_1_PENALTY=0.873570
PRIMER_LEFT_1_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_1_SEQUENCE=TTCATGGCCAAAAACTCACC
PRIMER_LEFT_1=918,20
PRIMER_RIGHT_1=1896,20
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.874
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=979
PRIMER_PAIR_2_PENALTY=1.189056
PRIMER_LEFT_2_PENALTY=0.085951
PRIMER_RIGHT_2_PENALTY=1.103105
PRIMER_LEFT_2_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_2_SEQUENCE=TCTTCATGGCCAAAAACTCAC
PRIMER_LEFT_2=918,20
PRIMER_RIGHT_2=1898,21
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=981
PRIMER_PAIR_3_PENALTY=1.189056
PRIMER_LEFT_3_PENALTY=0.085951
PRIMER_RIGHT_3_PENALTY=1.103105
PRIMER_LEFT_3_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_3_SEQUENCE=GTCTTCATGGCCAAAAACTCA
PRIMER_LEFT_3=918,20
PRIMER_RIGHT_3=1899,21
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=982
PRIMER_PAIR_4_PENALTY=1.707449
PRIMER_LEFT_4_PENALTY=0.954397
PRIMER_RIGHT_4_PENALTY=0.753052
PRIMER_LEFT_4_SEQUENCE=CCAAATGCCAGTGACAAGGT
PRIMER_RIGHT_4_SEQUENCE=TCTTCATGGCCAAAAACTCA
PRIMER_LEFT_4=905,20
PRIMER_RIGHT_4=1898,20
PRIMER_LEFT_4_TM=60.954
PRIMER_RIGHT_4_TM=59.247
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=994
PRIMER_PAIR_5_PENALTY=1.760032
PRIMER_LEFT_5_PENALTY=0.085951
PRIMER_RIGHT_5_PENALTY=1.674081
PRIMER_LEFT_5_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_5_SEQUENCE=CGCGTTCTCCTCAGTCTTCA
PRIMER_LEFT_5=918,20
PRIMER_RIGHT_5=1912,20
PRIMER_LEFT_5_TM=59.914
PRIMER_RIGHT_5_TM=61.674
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=995
PRIMER_PAIR_6_PENALTY=1.827967
PRIMER_LEFT_6_PENALTY=0.954397
PRIMER_RIGHT_6_PENALTY=0.873570
PRIMER_LEFT_6_SEQUENCE=CCAAATGCCAGTGACAAGGT
PRIMER_RIGHT_6_SEQUENCE=TTCATGGCCAAAAACTCACC
PRIMER_LEFT_6=905,20
PRIMER_RIGHT_6=1896,20
PRIMER_LEFT_6_TM=60.954
PRIMER_RIGHT_6_TM=60.874
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=992
PRIMER_PAIR_7_PENALTY=1.885105
PRIMER_LEFT_7_PENALTY=0.085951
PRIMER_RIGHT_7_PENALTY=1.799154
PRIMER_LEFT_7_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_7_SEQUENCE=CTTCATGGCCAAAAACTCAC
PRIMER_LEFT_7=918,20
PRIMER_RIGHT_7=1897,20
PRIMER_LEFT_7_TM=59.914
PRIMER_RIGHT_7_TM=58.201
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=980
PRIMER_PAIR_8_PENALTY=1.988925
PRIMER_LEFT_8_PENALTY=1.235873
PRIMER_RIGHT_8_PENALTY=0.753052
PRIMER_LEFT_8_SEQUENCE=AGCCAAATGCCAGTGACAAG
PRIMER_RIGHT_8_SEQUENCE=TCTTCATGGCCAAAAACTCA
PRIMER_LEFT_8=903,20
PRIMER_RIGHT_8=1898,20
PRIMER_LEFT_8_TM=61.236
PRIMER_RIGHT_8_TM=59.247
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=996
PRIMER_PAIR_9_PENALTY=2.057048
PRIMER_LEFT_9_PENALTY=1.303996
PRIMER_RIGHT_9_PENALTY=0.753052
PRIMER_LEFT_9_SEQUENCE=GCTAAGCCAAATGCCAGTGA
PRIMER_RIGHT_9_SEQUENCE=TCTTCATGGCCAAAAACTCA
PRIMER_LEFT_9=899,20
PRIMER_RIGHT_9=1898,20
PRIMER_LEFT_9_TM=61.304
PRIMER_RIGHT_9_TM=59.247
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=1000
PRIMER_PAIR_10_PENALTY=2.057502
PRIMER_LEFT_10_PENALTY=0.954397
PRIMER_RIGHT_10_PENALTY=1.103105
PRIMER_LEFT_10_SEQUENCE=CCAAATGCCAGTGACAAGGT
PRIMER_RIGHT_10_SEQUENCE=TCTTCATGGCCAAAAACTCAC
PRIMER_LEFT_10=905,20
PRIMER_RIGHT_10=1898,21
PRIMER_LEFT_10_TM=60.954
PRIMER_RIGHT_10_TM=60.103
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=994
PRIMER_PAIR_11_PENALTY=2.057502
PRIMER_LEFT_11_PENALTY=0.954397
PRIMER_RIGHT_11_PENALTY=1.103105
PRIMER_LEFT_11_SEQUENCE=CCAAATGCCAGTGACAAGGT
PRIMER_RIGHT_11_SEQUENCE=GTCTTCATGGCCAAAAACTCA
PRIMER_LEFT_11=905,20
PRIMER_RIGHT_11=1899,21
PRIMER_LEFT_11_TM=60.954
PRIMER_RIGHT_11_TM=60.103
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=995
PRIMER_PAIR_12_PENALTY=2.109442
PRIMER_LEFT_12_PENALTY=1.235873
PRIMER_RIGHT_12_PENALTY=0.873570
PRIMER_LEFT_12_SEQUENCE=AGCCAAATGCCAGTGACAAG
PRIMER_RIGHT_12_SEQUENCE=TTCATGGCCAAAAACTCACC
PRIMER_LEFT_12=903,20
PRIMER_RIGHT_12=1896,20
PRIMER_LEFT_12_TM=61.236
PRIMER_RIGHT_12_TM=60.874
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=994
PRIMER_PAIR_13_PENALTY=2.177565
PRIMER_LEFT_13_PENALTY=1.303996
PRIMER_RIGHT_13_PENALTY=0.873570
PRIMER_LEFT_13_SEQUENCE=GCTAAGCCAAATGCCAGTGA
PRIMER_RIGHT_13_SEQUENCE=TTCATGGCCAAAAACTCACC
PRIMER_LEFT_13=899,20
PRIMER_RIGHT_13=1896,20
PRIMER_LEFT_13_TM=61.304
PRIMER_RIGHT_13_TM=60.874
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=998
PRIMER_PAIR_14_PENALTY=2.338977
PRIMER_LEFT_14_PENALTY=1.235873
PRIMER_RIGHT_14_PENALTY=1.103105
PRIMER_LEFT_14_SEQUENCE=AGCCAAATGCCAGTGACAAG
PRIMER_RIGHT_14_SEQUENCE=TCTTCATGGCCAAAAACTCAC
PRIMER_LEFT_14=903,20
PRIMER_RIGHT_14=1898,21
PRIMER_LEFT_14_TM=61.236
PRIMER_RIGHT_14_TM=60.103
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=996
PRIMER_PAIR_15_PENALTY=2.338977
PRIMER_LEFT_15_PENALTY=1.235873
PRIMER_RIGHT_15_PENALTY=1.103105
PRIMER_LEFT_15_SEQUENCE=AGCCAAATGCCAGTGACAAG
PRIMER_RIGHT_15_SEQUENCE=GTCTTCATGGCCAAAAACTCA
PRIMER_LEFT_15=903,20
PRIMER_RIGHT_15=1899,21
PRIMER_LEFT_15_TM=61.236
PRIMER_RIGHT_15_TM=60.103
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=997
PRIMER_PAIR_16_PENALTY=2.380958
PRIMER_LEFT_16_PENALTY=0.085951
PRIMER_RIGHT_16_PENALTY=2.295006
PRIMER_LEFT_16_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_16_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_16=918,20
PRIMER_RIGHT_16=1660,21
PRIMER_LEFT_16_TM=59.914
PRIMER_RIGHT_16_TM=58.705
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=5.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=743
PRIMER_PAIR_17_PENALTY=2.434783
PRIMER_LEFT_17_PENALTY=0.085951
PRIMER_RIGHT_17_PENALTY=2.348831
PRIMER_LEFT_17_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_17_SEQUENCE=TTAGCCGCGTTCTCCTCAGT
PRIMER_LEFT_17=918,20
PRIMER_RIGHT_17=1917,20
PRIMER_LEFT_17_TM=59.914
PRIMER_RIGHT_17_TM=62.349
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=1000
PRIMER_PAIR_18_PENALTY=2.499618
PRIMER_LEFT_18_PENALTY=0.085951
PRIMER_RIGHT_18_PENALTY=2.413667
PRIMER_LEFT_18_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_18_SEQUENCE=CCGCGTTCTCCTCAGTCTTC
PRIMER_LEFT_18=918,20
PRIMER_RIGHT_18=1913,20
PRIMER_LEFT_18_TM=59.914
PRIMER_RIGHT_18_TM=62.414
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=60.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=996
PRIMER_PAIR_19_PENALTY=2.753551
PRIMER_LEFT_19_PENALTY=0.954397
PRIMER_RIGHT_19_PENALTY=1.799154
PRIMER_LEFT_19_SEQUENCE=CCAAATGCCAGTGACAAGGT
PRIMER_RIGHT_19_SEQUENCE=CTTCATGGCCAAAAACTCAC
PRIMER_LEFT_19=905,20
PRIMER_RIGHT_19=1897,20
PRIMER_LEFT_19_TM=60.954
PRIMER_RIGHT_19_TM=58.201
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=993
PRIMER_PAIR_20_PENALTY=2.851001
PRIMER_LEFT_20_PENALTY=0.085951
PRIMER_RIGHT_20_PENALTY=2.765049
PRIMER_LEFT_20_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_20_SEQUENCE=CTTCATGGCCAAAAACTCACC
PRIMER_LEFT_20=918,20
PRIMER_RIGHT_20=1897,21
PRIMER_LEFT_20_TM=59.914
PRIMER_RIGHT_20_TM=61.765
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=980
PRIMER_PAIR_21_PENALTY=2.953884
PRIMER_LEFT_21_PENALTY=0.085951
PRIMER_RIGHT_21_PENALTY=2.867932
PRIMER_LEFT_21_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_21_SEQUENCE=ATGGCCAAAAACTCACCCCT
PRIMER_LEFT_21=918,20
PRIMER_RIGHT_21=1893,20
PRIMER_LEFT_21_TM=59.914
PRIMER_RIGHT_21_TM=62.868
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=10.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=976
PRIMER_PAIR_22_PENALTY=2.983340
PRIMER_LEFT_22_PENALTY=2.230287
PRIMER_RIGHT_22_PENALTY=0.753052
PRIMER_LEFT_22_SEQUENCE=GTGACAAGGTTGTTCCTGCT
PRIMER_RIGHT_22_SEQUENCE=TCTTCATGGCCAAAAACTCA
PRIMER_LEFT_22=915,20
PRIMER_RIGHT_22=1898,20
PRIMER_LEFT_22_TM=57.770
PRIMER_RIGHT_22_TM=59.247
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=984
PRIMER_PAIR_23_PENALTY=3.008681
PRIMER_LEFT_23_PENALTY=0.085951
PRIMER_RIGHT_23_PENALTY=2.922730
PRIMER_LEFT_23_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_23_SEQUENCE=TAGCCGCGTTCTCCTCAGTC
PRIMER_LEFT_23=918,20
PRIMER_RIGHT_23=1916,20
PRIMER_LEFT_23_TM=59.914
PRIMER_RIGHT_23_TM=62.923
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=60.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=999
PRIMER_PAIR_24_PENALTY=3.035026
PRIMER_LEFT_24_PENALTY=1.235873
PRIMER_RIGHT_24_PENALTY=1.799154
PRIMER_LEFT_24_SEQUENCE=AGCCAAATGCCAGTGACAAG
PRIMER_RIGHT_24_SEQUENCE=CTTCATGGCCAAAAACTCAC
PRIMER_LEFT_24=903,20
PRIMER_RIGHT_24=1897,20
PRIMER_LEFT_24_TM=61.236
PRIMER_RIGHT_24_TM=58.201
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=995
PRIMER_PAIR_25_PENALTY=3.103857
PRIMER_LEFT_25_PENALTY=2.230287
PRIMER_RIGHT_25_PENALTY=0.873570
PRIMER_LEFT_25_SEQUENCE=GTGACAAGGTTGTTCCTGCT
PRIMER_RIGHT_25_SEQUENCE=TTCATGGCCAAAAACTCACC
PRIMER_LEFT_25=915,20
PRIMER_RIGHT_25=1896,20
PRIMER_LEFT_25_TM=57.770
PRIMER_RIGHT_25_TM=60.874
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=982
PRIMER_PAIR_26_PENALTY=3.207170
PRIMER_LEFT_26_PENALTY=0.085951
PRIMER_RIGHT_26_PENALTY=3.121218
PRIMER_LEFT_26_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_26_SEQUENCE=TCATGGCCAAAAACTCACCC
PRIMER_LEFT_26=918,20
PRIMER_RIGHT_26=1895,20
PRIMER_LEFT_26_TM=59.914
PRIMER_RIGHT_26_TM=63.121
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=9.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=978
PRIMER_PAIR_27_PENALTY=3.249404
PRIMER_LEFT_27_PENALTY=0.954397
PRIMER_RIGHT_27_PENALTY=2.295006
PRIMER_LEFT_27_SEQUENCE=CCAAATGCCAGTGACAAGGT
PRIMER_RIGHT_27_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_27=905,20
PRIMER_RIGHT_27=1660,21
PRIMER_LEFT_27_TM=60.954
PRIMER_RIGHT_27_TM=58.705
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=5.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=756
PRIMER_PAIR_28_PENALTY=3.333392
PRIMER_LEFT_28_PENALTY=2.230287
PRIMER_RIGHT_28_PENALTY=1.103105
PRIMER_LEFT_28_SEQUENCE=GTGACAAGGTTGTTCCTGCT
PRIMER_RIGHT_28_SEQUENCE=TCTTCATGGCCAAAAACTCAC
PRIMER_LEFT_28=915,20
PRIMER_RIGHT_28=1898,21
PRIMER_LEFT_28_TM=57.770
PRIMER_RIGHT_28_TM=60.103
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=984
PRIMER_PAIR_29_PENALTY=3.333392
PRIMER_LEFT_29_PENALTY=2.230287
PRIMER_RIGHT_29_PENALTY=1.103105
PRIMER_LEFT_29_SEQUENCE=GTGACAAGGTTGTTCCTGCT
PRIMER_RIGHT_29_SEQUENCE=GTCTTCATGGCCAAAAACTCA
PRIMER_LEFT_29=915,20
PRIMER_RIGHT_29=1899,21
PRIMER_LEFT_29_TM=57.770
PRIMER_RIGHT_29_TM=60.103
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=985
PRIMER_PAIR_30_PENALTY=3.335266
PRIMER_LEFT_30_PENALTY=2.582214
PRIMER_RIGHT_30_PENALTY=0.753052
PRIMER_LEFT_30_SEQUENCE=TTCCTGCTGCTGCTGAAATG
PRIMER_RIGHT_30_SEQUENCE=TCTTCATGGCCAAAAACTCA
PRIMER_LEFT_30=927,20
PRIMER_RIGHT_30=1898,20
PRIMER_LEFT_30_TM=62.582
PRIMER_RIGHT_30_TM=59.247
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.2000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=972
PRIMER_PAIR_31_PENALTY=3.366497
PRIMER_LEFT_31_PENALTY=0.059762
PRIMER_RIGHT_31_PENALTY=3.306735
PRIMER_LEFT_31_SEQUENCE=AATATGCAAAGCTCGGATGG
PRIMER_RIGHT_31_SEQUENCE=AAGATTCTGCTTTTGTGCCT
PRIMER_LEFT_31=559,20
PRIMER_RIGHT_31=1425,20
PRIMER_LEFT_31_TM=60.060
PRIMER_RIGHT_31_TM=56.693
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=867
PRIMER_PAIR_32_PENALTY=3.392687
PRIMER_LEFT_32_PENALTY=0.085951
PRIMER_RIGHT_32_PENALTY=3.306735
PRIMER_LEFT_32_SEQUENCE=ACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_32_SEQUENCE=AAGATTCTGCTTTTGTGCCT
PRIMER_LEFT_32=918,20
PRIMER_RIGHT_32=1425,20
PRIMER_LEFT_32_TM=59.914
PRIMER_RIGHT_32_TM=56.693
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=508
PRIMER_PAIR_33_PENALTY=3.395780
PRIMER_LEFT_33_PENALTY=1.100774
PRIMER_RIGHT_33_PENALTY=2.295006
PRIMER_LEFT_33_SEQUENCE=CAACAAGCAGAGAACCCAAAG
PRIMER_RIGHT_33_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_33=845,21
PRIMER_RIGHT_33=1660,21
PRIMER_LEFT_33_TM=59.899
PRIMER_RIGHT_33_TM=58.705
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=5.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=816
PRIMER_PAIR_34_PENALTY=3.397906
PRIMER_LEFT_34_PENALTY=1.102899
PRIMER_RIGHT_34_PENALTY=2.295006
PRIMER_LEFT_34_SEQUENCE=CAACAAGCAGAGAACCCAAA
PRIMER_RIGHT_34_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_34=845,20
PRIMER_RIGHT_34=1660,21
PRIMER_LEFT_34_TM=58.897
PRIMER_RIGHT_34_TM=58.705
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=5.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=816
PRIMER_PAIR_35_PENALTY=3.455783
PRIMER_LEFT_35_PENALTY=2.582214
PRIMER_RIGHT_35_PENALTY=0.873570
PRIMER_LEFT_35_SEQUENCE=TTCCTGCTGCTGCTGAAATG
PRIMER_RIGHT_35_SEQUENCE=TTCATGGCCAAAAACTCACC
PRIMER_LEFT_35=927,20
PRIMER_RIGHT_35=1896,20
PRIMER_LEFT_35_TM=62.582
PRIMER_RIGHT_35_TM=60.874
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.2000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=970
PRIMER_PAIR_36_PENALTY=3.456130
PRIMER_LEFT_36_PENALTY=0.149395
PRIMER_RIGHT_36_PENALTY=3.306735
PRIMER_LEFT_36_SEQUENCE=GCAACTTCCTCAACCGTCTC
PRIMER_RIGHT_36_SEQUENCE=AAGATTCTGCTTTTGTGCCT
PRIMER_LEFT_36=617,20
PRIMER_RIGHT_36=1425,20
PRIMER_LEFT_36_TM=59.851
PRIMER_RIGHT_36_TM=56.693
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.1000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=809
PRIMER_PAIR_37_PENALTY=3.515840
PRIMER_LEFT_37_PENALTY=2.762788
PRIMER_RIGHT_37_PENALTY=0.753052
PRIMER_LEFT_37_SEQUENCE=GACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_37_SEQUENCE=TCTTCATGGCCAAAAACTCA
PRIMER_LEFT_37=917,21
PRIMER_RIGHT_37=1898,20
PRIMER_LEFT_37_TM=61.763
PRIMER_RIGHT_37_TM=59.247
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=982
PRIMER_PAIR_38_PENALTY=3.528103
PRIMER_LEFT_38_PENALTY=0.221368
PRIMER_RIGHT_38_PENALTY=3.306735
PRIMER_LEFT_38_SEQUENCE=GAAACTTCCGCATCTGCATT
PRIMER_RIGHT_38_SEQUENCE=AAGATTCTGCTTTTGTGCCT
PRIMER_LEFT_38=480,20
PRIMER_RIGHT_38=1425,20
PRIMER_LEFT_38_TM=60.221
PRIMER_RIGHT_38_TM=56.693
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.4000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=946
PRIMER_PAIR_39_PENALTY=3.529349
PRIMER_LEFT_39_PENALTY=1.234342
PRIMER_RIGHT_39_PENALTY=2.295006
PRIMER_LEFT_39_SEQUENCE=ACAACAAGCAGAGAACCCAAA
PRIMER_RIGHT_39_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_39=844,21
PRIMER_RIGHT_39=1660,21
PRIMER_LEFT_39_TM=59.766
PRIMER_RIGHT_39_TM=58.705
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.8000
PRIMER_RIGHT_39_END_STABILITY=5.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=817
PRIMER_PAIR_40_PENALTY=3.529349
PRIMER_LEFT_40_PENALTY=1.234342
PRIMER_RIGHT_40_PENALTY=2.295006
PRIMER_LEFT_40_SEQUENCE=AACAACAAGCAGAGAACCCAA
PRIMER_RIGHT_40_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_40=843,21
PRIMER_RIGHT_40=1660,21
PRIMER_LEFT_40_TM=59.766
PRIMER_RIGHT_40_TM=58.705
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=10.0000
PRIMER_RIGHT_40_END_STABILITY=5.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=818
PRIMER_PAIR_41_PENALTY=3.529349
PRIMER_LEFT_41_PENALTY=1.234342
PRIMER_RIGHT_41_PENALTY=2.295006
PRIMER_LEFT_41_SEQUENCE=AAACAACAAGCAGAGAACCCA
PRIMER_RIGHT_41_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_41=842,21
PRIMER_RIGHT_41=1660,21
PRIMER_LEFT_41_TM=59.766
PRIMER_RIGHT_41_TM=58.705
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=5.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=819
PRIMER_PAIR_42_PENALTY=3.530879
PRIMER_LEFT_42_PENALTY=1.235873
PRIMER_RIGHT_42_PENALTY=2.295006
PRIMER_LEFT_42_SEQUENCE=AGCCAAATGCCAGTGACAAG
PRIMER_RIGHT_42_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_42=903,20
PRIMER_RIGHT_42=1660,21
PRIMER_LEFT_42_TM=61.236
PRIMER_RIGHT_42_TM=58.705
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=5.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=758
PRIMER_PAIR_43_PENALTY=3.550535
PRIMER_LEFT_43_PENALTY=0.243800
PRIMER_RIGHT_43_PENALTY=3.306735
PRIMER_LEFT_43_SEQUENCE=AAGGCATCCAACAATCATCC
PRIMER_RIGHT_43_SEQUENCE=AAGATTCTGCTTTTGTGCCT
PRIMER_LEFT_43=648,20
PRIMER_RIGHT_43=1425,20
PRIMER_LEFT_43_TM=59.756
PRIMER_RIGHT_43_TM=56.693
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=778
PRIMER_PAIR_44_PENALTY=3.599002
PRIMER_LEFT_44_PENALTY=1.303996
PRIMER_RIGHT_44_PENALTY=2.295006
PRIMER_LEFT_44_SEQUENCE=GCTAAGCCAAATGCCAGTGA
PRIMER_RIGHT_44_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_44=899,20
PRIMER_RIGHT_44=1660,21
PRIMER_LEFT_44_TM=61.304
PRIMER_RIGHT_44_TM=58.705
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=5.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=762
PRIMER_PAIR_45_PENALTY=3.636358
PRIMER_LEFT_45_PENALTY=2.762788
PRIMER_RIGHT_45_PENALTY=0.873570
PRIMER_LEFT_45_SEQUENCE=GACAAGGTTGTTCCTGCTGCT
PRIMER_RIGHT_45_SEQUENCE=TTCATGGCCAAAAACTCACC
PRIMER_LEFT_45=917,21
PRIMER_RIGHT_45=1896,20
PRIMER_LEFT_45_TM=61.763
PRIMER_RIGHT_45_TM=60.874
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=980
PRIMER_PAIR_46_PENALTY=3.685318
PRIMER_LEFT_46_PENALTY=2.582214
PRIMER_RIGHT_46_PENALTY=1.103105
PRIMER_LEFT_46_SEQUENCE=TTCCTGCTGCTGCTGAAATG
PRIMER_RIGHT_46_SEQUENCE=TCTTCATGGCCAAAAACTCAC
PRIMER_LEFT_46=927,20
PRIMER_RIGHT_46=1898,21
PRIMER_LEFT_46_TM=62.582
PRIMER_RIGHT_46_TM=60.103
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.2000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=972
PRIMER_PAIR_47_PENALTY=3.685318
PRIMER_LEFT_47_PENALTY=2.582214
PRIMER_RIGHT_47_PENALTY=1.103105
PRIMER_LEFT_47_SEQUENCE=TTCCTGCTGCTGCTGAAATG
PRIMER_RIGHT_47_SEQUENCE=GTCTTCATGGCCAAAAACTCA
PRIMER_LEFT_47=927,20
PRIMER_RIGHT_47=1899,21
PRIMER_LEFT_47_TM=62.582
PRIMER_RIGHT_47_TM=60.103
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.2000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=973
PRIMER_PAIR_48_PENALTY=3.719447
PRIMER_LEFT_48_PENALTY=0.954397
PRIMER_RIGHT_48_PENALTY=2.765049
PRIMER_LEFT_48_SEQUENCE=CCAAATGCCAGTGACAAGGT
PRIMER_RIGHT_48_SEQUENCE=CTTCATGGCCAAAAACTCACC
PRIMER_LEFT_48=905,20
PRIMER_RIGHT_48=1897,21
PRIMER_LEFT_48_TM=60.954
PRIMER_RIGHT_48_TM=61.765
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=993
PRIMER_PAIR_49_PENALTY=3.720946
PRIMER_LEFT_49_PENALTY=1.425940
PRIMER_RIGHT_49_PENALTY=2.295006
PRIMER_LEFT_49_SEQUENCE=TCAGCTAAGCCAAATGCCAG
PRIMER_RIGHT_49_SEQUENCE=CCAAGGGTCTCGTTTTCATAG
PRIMER_LEFT_49=896,20
PRIMER_RIGHT_49=1660,21
PRIMER_LEFT_49_TM=61.426
PRIMER_RIGHT_49_TM=58.705
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=5.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=765
=
SEQUENCE_ID=MAGIC.426_3_13894739_13894977_wil-2_oy-0_222884_83275_TRUE
SEQUENCE_TARGET=950,338
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_17175714_17176464_wil-2_zu-0_269341_104258_TRUE
SEQUENCE_TARGET=950,850
SEQUENCE_TEMPLATE=TAGCAACANAAAATNANTAATAGTCGTAAGNNANCAAGAAAGGAAANNAAATAAGNAGAGATACTTCACNTGAATTTGACTTAGNAANANAATCTCAAAGTTCTTAGTTACNAANTNNGAANAAAAGATGTTAATCGTGAAGNTANTGTTNTTTTNTTNTNGTGTCTAGTTTTTTGTTGTGCTNTATTTANTNTCTNGTNTTATGTTGTGTTTGAGTGTNNTNAGAGTTNNNNNNNNTGTNATGTGAATTGTTTTNNTGTTGCATTTGAGTTNTGTTNGTGTTATNNTTGACTTTTGTTNTNNTTGGNTGTGCTNTGAGTTTGAGTGCATTGTGTTTGTTGNNNNNNNNNTGTCTGTGAACGTGTNTNTTTCTAGATAGTGTGAGTAACNCNAACAAGTCAANNNNNNNNNNNNGAACTTATGCANGANANAATAGACATGAAATTAGAGGGAGAGATATCTGACCTCNCATATGATTTTGGCTACAAAATCACNTTCNAAATTTTTGTTCTCTGGNCCTCAAAGATATNTACATGAAGNAGGAGACACNACCCCTCATTTCCTTTTGCTTACTGAGCCNAAAACTTCTCTTGAAATACNGCACCTGAAAGTACAACANTAAATGAAACTANCCNAATAAATGCCAGGCCTTANAGAGCCNNNNNNNNACTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAGNACACATTAAGATAANAGAAAGAGACTCCANTNCATCATCTAGGCAACANANTATACTTTAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTCTNGCATGAATATNACAATCAACCANTTTTTTCCTTTTNCAAAATNAATCTTCACNAATTCACNNATNAAGACANTGATGGANTCATAATGACNCCACAACCAAAGAAAGATCCNTNAGTCCAGATTNNNNNNNNNNNNTTGCTTCCTTAAAATCCCAAAACGATACAATCAAAATCANNNNNNNNNNNNNNNNNNNTAAATCGTTGNTTTTTNTANCCATTCAGACTCATCAATTNCAACAAGTANNAGNGNNATNTAGACTCTNTTCAACTGTTTCAGAACATTAAAANTCTCCGATCACCAAATTTANAANCTATCGAANAAGNTANACATTANCCTAATAGAAAGATCCCAGAACCATCAAACCTCTAAATTNCAACAGAAATCAACGAAAGCAAATTCCAAATCCAAACCCTAGNNTCCCAAATTAACCAAGAANANAGAGANATCGAAACATTTCGTACCTTCTTCNATTTNGTANGACTATCACACGNTCNNGATTCAAACCAATAGACAAGTAATAATCNTTGCATNAATCNTCNGATTATCANCGACCCAAAATCAAAACCCTTTGATCCCNATTTGAANTTGANCCAANGAATCAGAGACGCAATTCNATTGANCGNAGAAAAGNATCNGAAGAAGATAGAANANGANAATCGCTTCTCCATGCGANTATGTAAACCCTAANGCNTTTTTCTCAAAGAGGATTTGAAGAAATGAATTNTGAAATTTCAACTTTCGCTAACNGTTACTTGTGGAAGGGGAAGAGTTAACGTTGGCAAATAGAGTNAGTGNGGCCAGNNCTATTTANCNGGNTCTTTTGAAACAATCAGCATNTTAATTTACGATTATGACCNTGANTTTTCAAAATTTGTTTCCCGCTTTTGAGTTAGAATCACATACACGCTAGAGTAGTTTAAACGTNNTGTAAACCACCAANCCAAACTAATCCTTTGGNCTTTANNNNNNNAAAAACNNNNNCNNNNAAANTAAATAANTCATTGGAGCTAGNNNNTGAAAACNTTACAACTTGTGNANTTTTTNATTTTAGTAGCACCAACAACACTTGGAGGTATGTNGTCGCTGCTCCTTGTNTTATNGATNGTANAAATANTCNATTATATTTAGATGGATCACTCTANTNGTTCACCAACGAGNATATCACANAAANAAAGATATTATCNTTCNATNTTCACANAGAAAAGTTTNAAATCATCTCAAAANCTCCGTTTGNCCAAGNAACTCGTAANCATATCATCATGTGTAACCTGAATAATCGTCTTTGCGTATCTNAAAAGAAGTGGTCAAGANAAGAGATATGGNCATTAGANAATTCTGNCATGACATGGGAGAAAATNTATTNGTTTGATNTNCCAGTAANANTTGATTATNATTCGTTNTATGANNANNTCTACNCATGTGTCACTCNGATCATGNTTATGAANAATAANAANTCATTANTGCTTTTGNATACAAATGTCAATGATCACANTCTTGNTATANTCGACCCTGAAATGAANNNCTANANTTTNTNTTTCACACNNGGNTNTTACTCATANGNNCTTTCTTGTAATCAAAGCTTGNTGTCANCTGAATAAAAACAGTACANTTCNTTTTNTNAGTANTTNTTNNTATATATTTGGTNTNCATNANANNNTNTTGTNCTCAACTTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_12744938_12745405_oy-0_wu-0_469790_17827_TRUE
SEQUENCE_TARGET=950,567
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_13983280_13983285_wil-2_ct-1_7469_21982_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_10911894_10911920_ler-0_oy-0_76828_690398_TRUE
SEQUENCE_TARGET=950,126
SEQUENCE_TEMPLATE=ACTCACCGAGNCATCGATNGAAATCGAGTGGNNATAATGAGTCCATTCAGAAGTTGGCAANTTNTTGAACTTGCGAANACGNNNTTGATCATCAAATGTCANAGTATTAGTGGCCTTCAAACGAANATGCTTNAATGNNTTANNGGGNAATGACGTNANGTGAAAGCCATAGAGTTTGCTTAACGGNCATAANTTGGTCGAAGAGCTAAGAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_8513745_8513754_bur-0_wil-2_178_48223_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=GCTNCTTTTTGTGGCATATTGATGAAATATTAATNTTACACTTGGCTATTTTCATTGATCTGCTTAATGTTNCTGTTTATTGGTTTCATACGTGAATACAATCTAAACCAAATGATTGTGTAAATGTTTAGGTGGTGGACAACTACTACAGGCCGGACTTGAAGAAAGCTGCACTTGCTAGACTCAGCGCCATCAGCAAAGGTCTCAGGGTTGCCAAGTCNGGTGCCAAGCAAAGAAACAGACAAGCTTAAGCTTCTTCTTTCACAATCTGNTTTTTTTGAAGTAAAAGATTTTGTTGAACAGGTTTTATTTAAGTTGTTGCTTTGAAGTTGAACCATAANGCTATTCTCCTTTTTCNTTATCCTTATTATAAAGGAGTTAGAAGTTTATCTTNTTANNNNNNNNNNNNNNNNNNTGCTTGTGTTACATTTTGTTANATCNTTTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAACNAAGTCCTTATATNTNATGANNTTTGNAAGCCTANANNNNNNNNNNNNNNNNNNNNNNNNNNTTTANNTTNNTTGCATCANATGTGATGTTCTTGTGGACTNTAATGTTGACNTTNTCNTTNGATCCCATCAAAAACNAACTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACNGAAAGGTTTATATTAGGGTTTCNTAGACNTGNNTTAATTNTGTNCTCTNGCTGTNGCCGTCTCCAACNCCATTTCGAAGNNNNGNGAGANCGANGCNNNANNGAGNAAGNAAANNGNAGAAAGGAANNGGNNGNNANNANGGAGNNCNNNANCANNNNNNACANNNNNCTCCANAGNNNGTANNNNNTAAANCNTNAANNNTNNNNANCTTATNTNCANTNTCTCGTTTCCGNTTTNNTTAAGTTCGTTCTNGGNNTAGNNNNTAANNATTAGACTNAAATTGCAACTTNTNTTGATTNNAATTAGGTTTCTTGATGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANTCTNTAGATGAGTAANGATCATAAANATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTNGNNNCCTTTTATTGNATAGAAAGGTTGGCTTGTTGCTTAGGTNCATTTTNNATTNGAGNTTTTTTCGTTGTCANNATTGATCNNGTGGTNNNNNNNNNNNNNNNNNNNNTNNTTTCTAAATTGAACAGCACCTTCAAGAAGAAGGCACCCAATGCCATTAAAGAGATCAGGAAGTTTGCATTGAAAGCAATGGGAACNAANGACGTTAGAGTNGATGTTAAACTCAACAAGCAGATATGGAGCAAAGGTATCCGAGGTCCCCCGNGGAGNATNAGAGTCCGTGTTGCCCGTAAGAGAAACGATGATGAAGATGCCAAGGAAGAGTTCTTCTCNCTCGTCACNGTCGCTGAGATTCCGGCTGAAGGATTATCTGGTTTGGGTACTAAGGTCATTGAAGAAGAGGAGTGAAAATTGGTGAATGTACACTCACACANCCTTATCTTTNGATGNAAAGACGAAANNAAAAGACTTTGCTTGTTTTGATCACTTACCTATTTTGGTCATTTATCATNTCTTATGTTTTAAGTTCTATAGACAACAGATTTGGTACTACTCATACGAGTTTGATCTTTTTGTGACGCANTTTACTTCGCAATCATCTTTCTGATTCTGTACCAGACCGGTTTATTGTCTGGGTTGAAGTGTAACGAATTGGTTTGATACTCCACATTGTTAGGTTGGTGGAGTNTATCATAACTACTAAAAGGGTAGATAATCCNAAANTCCATATAAAATCAANGAGTATTACAAATNTNAAGGAAAAAATATTTACTTNTGTTCTAAATNAAATTAATCCGCAACTTTTNACTGAAGTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_11096793_11096925_sf-2_hi-0_9546_2694360_TRUE
SEQUENCE_TARGET=950,232
SEQUENCE_TEMPLATE=AAAGGCTNAAGCCNAGTAAATANGTCGAACNGANTAAACCCGAGTTGGGGAGTTCGCGGATTCAAGTNCGCATTNCTTAGAGATAGGTNTGATCAACNTACGAATCTCGTGACTTGACNACATGGTTAAACAAAAATGCTATATAAAAGGTGGANTTTGCNAATCNATACTTGAATCNAGCGCAAAGAAANCCCTCTTNGGTTCATAGNCTTAAAAAATAATAATAAAGNNTTAAAAGCCTCCTCAGGCCAAGTATTTGTTTAGCCTACTTAGGCAGGTAAAATAGTAATAGTAAACTTTNAGTCTGTTNACAAAAAGGNACAATCCCCATTAAGTGGACANANCCACTACNGNTNCTCAGGCACCTCGAGTTCGGGTTGAGCNGNCTCANGATCAACTNAATCGGCGNCAGCTNTCTCNNTCTCACCCGNCTCGCCNNTAGTAGGCTGCTCGNTAGGAACCNTGGAATCTGAACCATCATCNTCCTCGATGACCACTGTNTCCGAGTTCNTGGATGGAGGAATNTCTGGCGGNGTGGNTACCGCCTCCGGAATTNTCNTNGGCACGATGGATGCTTCGAGCNCCACGGGATCGCTAGCACCTGAGNCAATTATCTCGGATTAGGAAAAATNCAAGAGTAATTNGCTGTGNATTGGTTTCAAAAGTTTAGGAATGTACNAGCCCAATCTAAANCTGGCATCNTTTAAGAACGGAGATCGAACTCAAGGGACGAAGANTTGTCTTTCCACGTCATAACCTCNTTTTCCCTGTCTAGCAACAGTCTCTCGTANTACTCAATCATGTCATTNAGTTCGCNGTTTGCCTGAACNAAGTNGTAACAAAAAAGAGTTAGCATCTAAGTGNAGGATTAAAGTTTAAANNNNNATTATGGGTGAAGNGAACTAACCNGAGATGAGTGATGGGNNTGGAGTAGGAATNGCNTGCGAATTGCAGGACGCCANCTATCAAANNNNGGCAGGATGGTGTCTTTTCAAGNTTTNAAGCACCGAAAGACGTTTCCCTCTGTTTTCATCGCTGTTAGAATAAAGAAAGGGTTGGACCAGGCAAGGAGCGTTNAGTCANTTAATCGAAATGATAAGGCCNGAAAAAAAAATNNAAGGTAATTTCTAACNCGTGAACGACTTGGTCTGAGGAACNANAACAGCCTNAGTTCNNGCATCTCGGACCTCAGCTTCTTCTCNNNGGGGGTTTGCACTCTGATCNNCCTTTTCACCTCTGGATCCAGACNANGGAGATACGGTGACTGGAGGANGAGGNCCCATAGAGCGGGGTGGTGGCGATGGCCGAGGAGAGNGATCAGTTCGGGCTCTCGTCNTATCTCTTTTGGGGGAGGAGCTACCNCGGGTTGATCTGTCTCTCTATTTCNGAATNAGGTCAGCAAGCGGCTCGCTGCTTAGTCTAGTTCGGGAGGCAGGCCGAGGTGATTNGNGGCGCGGTGATCGTTCTCTNCNGGATGACTGGCCGCTATGGCCTTCGCGGTGAGGAATGGGTCTGTAGGCACCAGCAGATNTTGCCCCTTCGGCGCTNCCATNGTCANCTAGCAAGGTTCTGCTATATTNTTTCTTGTCGACTGCGGATTGAAGGAATGATTTGTTCGTTTNGCAAGCCTCCACGATCGACTTGGATAGCNTCTNGCGTCGAGNTGCGATGACCCGAGCAGAAGTCCCTTGAGGTAAGGGAGGGGGCAATGGCAGTTGGAGAAGATGTCNAGGGGGGCTCATNCTTAGCTTGTGGTNNGCCTCTACGAATCNGTCGAGAGTNAAGGAGTTCCAGAGAGTTCNAGAGAGTTTCCCNTTCGGCNAATTCAANAGTCGGAAGGGTCGGTGCGATGAAAGCTGCNAATTGAAAATTAAGAGTTGGTNANATAAGGNAAGAAGTAAATAANGGTCATATGCATGACCTAACATCTANNGGGCTTAGTAGTCNACTTCGAGGAGAGCAAGTCCGAAAGGTTTCCAATCGAAGCNNGNCTCTTTTTGATTAAGNACCACNNGTGATGCCAGTTCTCGTCNTTATTAGGGAGATGCGAGATNAGGTTCCTGTTNGCNTTCNGATNGGCCGAGAAGTATCCTACCTTCTTTGAAGAACTCCTCACACNGAGAAGTTCANTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.205903
PRIMER_LEFT_0_PENALTY=0.195358
PRIMER_RIGHT_0_PENALTY=0.010545
PRIMER_LEFT_0_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_0_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_0=714,20
PRIMER_RIGHT_0=1524,20
PRIMER_LEFT_0_TM=60.195
PRIMER_RIGHT_0_TM=59.989
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=811
PRIMER_PAIR_1_PENALTY=0.207448
PRIMER_LEFT_1_PENALTY=0.196903
PRIMER_RIGHT_1_PENALTY=0.010545
PRIMER_LEFT_1_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_1_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_1=713,20
PRIMER_RIGHT_1=1524,20
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.989
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=812
PRIMER_PAIR_2_PENALTY=0.268309
PRIMER_LEFT_2_PENALTY=0.195358
PRIMER_RIGHT_2_PENALTY=0.072951
PRIMER_LEFT_2_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_2_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_2=714,20
PRIMER_RIGHT_2=1519,20
PRIMER_LEFT_2_TM=60.195
PRIMER_RIGHT_2_TM=60.073
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=806
PRIMER_PAIR_3_PENALTY=0.269854
PRIMER_LEFT_3_PENALTY=0.196903
PRIMER_RIGHT_3_PENALTY=0.072951
PRIMER_LEFT_3_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_3_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_3=713,20
PRIMER_RIGHT_3=1519,20
PRIMER_LEFT_3_TM=59.803
PRIMER_RIGHT_3_TM=60.073
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=807
PRIMER_PAIR_4_PENALTY=0.269854
PRIMER_LEFT_4_PENALTY=0.196903
PRIMER_RIGHT_4_PENALTY=0.072951
PRIMER_LEFT_4_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_4_SEQUENCE=TGCCTACAGACCCATTCCTC
PRIMER_LEFT_4=713,20
PRIMER_RIGHT_4=1520,20
PRIMER_LEFT_4_TM=59.803
PRIMER_RIGHT_4_TM=60.073
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=808
PRIMER_PAIR_5_PENALTY=0.665358
PRIMER_LEFT_5_PENALTY=0.195358
PRIMER_RIGHT_5_PENALTY=0.470001
PRIMER_LEFT_5_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_5_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_5=714,20
PRIMER_RIGHT_5=1270,20
PRIMER_LEFT_5_TM=60.195
PRIMER_RIGHT_5_TM=59.530
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=60.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=557
PRIMER_PAIR_6_PENALTY=0.666904
PRIMER_LEFT_6_PENALTY=0.196903
PRIMER_RIGHT_6_PENALTY=0.470001
PRIMER_LEFT_6_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_6_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_6=713,20
PRIMER_RIGHT_6=1270,20
PRIMER_LEFT_6_TM=59.803
PRIMER_RIGHT_6_TM=59.530
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=558
PRIMER_PAIR_7_PENALTY=0.726671
PRIMER_LEFT_7_PENALTY=0.716126
PRIMER_RIGHT_7_PENALTY=0.010545
PRIMER_LEFT_7_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_7_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_7=715,20
PRIMER_RIGHT_7=1524,20
PRIMER_LEFT_7_TM=59.284
PRIMER_RIGHT_7_TM=59.989
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.7000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=810
PRIMER_PAIR_8_PENALTY=0.764708
PRIMER_LEFT_8_PENALTY=0.195358
PRIMER_RIGHT_8_PENALTY=0.569350
PRIMER_LEFT_8_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_8_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_8=714,20
PRIMER_RIGHT_8=1359,20
PRIMER_LEFT_8_TM=60.195
PRIMER_RIGHT_8_TM=60.569
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=646
PRIMER_PAIR_9_PENALTY=0.766253
PRIMER_LEFT_9_PENALTY=0.196903
PRIMER_RIGHT_9_PENALTY=0.569350
PRIMER_LEFT_9_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_9_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_9=713,20
PRIMER_RIGHT_9=1359,20
PRIMER_LEFT_9_TM=59.803
PRIMER_RIGHT_9_TM=60.569
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=647
PRIMER_PAIR_10_PENALTY=0.789077
PRIMER_LEFT_10_PENALTY=0.716126
PRIMER_RIGHT_10_PENALTY=0.072951
PRIMER_LEFT_10_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_10_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_10=715,20
PRIMER_RIGHT_10=1519,20
PRIMER_LEFT_10_TM=59.284
PRIMER_RIGHT_10_TM=60.073
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.7000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=805
PRIMER_PAIR_11_PENALTY=0.789077
PRIMER_LEFT_11_PENALTY=0.716126
PRIMER_RIGHT_11_PENALTY=0.072951
PRIMER_LEFT_11_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_11_SEQUENCE=TGCCTACAGACCCATTCCTC
PRIMER_LEFT_11=715,20
PRIMER_RIGHT_11=1520,20
PRIMER_LEFT_11_TM=59.284
PRIMER_RIGHT_11_TM=60.073
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.7000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=806
PRIMER_PAIR_12_PENALTY=0.889206
PRIMER_LEFT_12_PENALTY=0.195358
PRIMER_RIGHT_12_PENALTY=0.693848
PRIMER_LEFT_12_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_12_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_12=714,20
PRIMER_RIGHT_12=1358,20
PRIMER_LEFT_12_TM=60.195
PRIMER_RIGHT_12_TM=60.694
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=645
PRIMER_PAIR_13_PENALTY=0.890751
PRIMER_LEFT_13_PENALTY=0.196903
PRIMER_RIGHT_13_PENALTY=0.693848
PRIMER_LEFT_13_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_13_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_13=713,20
PRIMER_RIGHT_13=1358,20
PRIMER_LEFT_13_TM=59.803
PRIMER_RIGHT_13_TM=60.694
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=646
PRIMER_PAIR_14_PENALTY=0.923246
PRIMER_LEFT_14_PENALTY=0.912701
PRIMER_RIGHT_14_PENALTY=0.010545
PRIMER_LEFT_14_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_14_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_14=716,20
PRIMER_RIGHT_14=1524,20
PRIMER_LEFT_14_TM=60.913
PRIMER_RIGHT_14_TM=59.989
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=809
PRIMER_PAIR_15_PENALTY=0.971076
PRIMER_LEFT_15_PENALTY=0.195358
PRIMER_RIGHT_15_PENALTY=0.775718
PRIMER_LEFT_15_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_15_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_15=714,20
PRIMER_RIGHT_15=1599,20
PRIMER_LEFT_15_TM=60.195
PRIMER_RIGHT_15_TM=60.776
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=886
PRIMER_PAIR_16_PENALTY=0.972621
PRIMER_LEFT_16_PENALTY=0.196903
PRIMER_RIGHT_16_PENALTY=0.775718
PRIMER_LEFT_16_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_16_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_16=713,20
PRIMER_RIGHT_16=1599,20
PRIMER_LEFT_16_TM=59.803
PRIMER_RIGHT_16_TM=60.776
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=887
PRIMER_PAIR_17_PENALTY=0.985652
PRIMER_LEFT_17_PENALTY=0.912701
PRIMER_RIGHT_17_PENALTY=0.072951
PRIMER_LEFT_17_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_17_SEQUENCE=GCCTACAGACCCATTCCTCA
PRIMER_LEFT_17=716,20
PRIMER_RIGHT_17=1519,20
PRIMER_LEFT_17_TM=60.913
PRIMER_RIGHT_17_TM=60.073
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=804
PRIMER_PAIR_18_PENALTY=0.985652
PRIMER_LEFT_18_PENALTY=0.912701
PRIMER_RIGHT_18_PENALTY=0.072951
PRIMER_LEFT_18_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_18_SEQUENCE=TGCCTACAGACCCATTCCTC
PRIMER_LEFT_18=716,20
PRIMER_RIGHT_18=1520,20
PRIMER_LEFT_18_TM=60.913
PRIMER_RIGHT_18_TM=60.073
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=805
PRIMER_PAIR_19_PENALTY=1.119143
PRIMER_LEFT_19_PENALTY=0.195358
PRIMER_RIGHT_19_PENALTY=0.923785
PRIMER_LEFT_19_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_19_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_19=714,20
PRIMER_RIGHT_19=1523,20
PRIMER_LEFT_19_TM=60.195
PRIMER_RIGHT_19_TM=60.924
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=810
PRIMER_PAIR_20_PENALTY=1.120688
PRIMER_LEFT_20_PENALTY=0.196903
PRIMER_RIGHT_20_PENALTY=0.923785
PRIMER_LEFT_20_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_20_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_20=713,20
PRIMER_RIGHT_20=1523,20
PRIMER_LEFT_20_TM=59.803
PRIMER_RIGHT_20_TM=60.924
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=811
PRIMER_PAIR_21_PENALTY=1.177619
PRIMER_LEFT_21_PENALTY=0.195358
PRIMER_RIGHT_21_PENALTY=0.982262
PRIMER_LEFT_21_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_21_SEQUENCE=GTGCCTACAGACCCATTCCT
PRIMER_LEFT_21=714,20
PRIMER_RIGHT_21=1521,20
PRIMER_LEFT_21_TM=60.195
PRIMER_RIGHT_21_TM=59.018
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=808
PRIMER_PAIR_22_PENALTY=1.179165
PRIMER_LEFT_22_PENALTY=0.196903
PRIMER_RIGHT_22_PENALTY=0.982262
PRIMER_LEFT_22_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_22_SEQUENCE=GTGCCTACAGACCCATTCCT
PRIMER_LEFT_22=713,20
PRIMER_RIGHT_22=1521,20
PRIMER_LEFT_22_TM=59.803
PRIMER_RIGHT_22_TM=59.018
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=809
PRIMER_PAIR_23_PENALTY=1.186127
PRIMER_LEFT_23_PENALTY=0.716126
PRIMER_RIGHT_23_PENALTY=0.470001
PRIMER_LEFT_23_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_23_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_23=715,20
PRIMER_RIGHT_23=1270,20
PRIMER_LEFT_23_TM=59.284
PRIMER_RIGHT_23_TM=59.530
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=60.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.7000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=556
PRIMER_PAIR_24_PENALTY=1.285476
PRIMER_LEFT_24_PENALTY=0.716126
PRIMER_RIGHT_24_PENALTY=0.569350
PRIMER_LEFT_24_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_24_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_24=715,20
PRIMER_RIGHT_24=1359,20
PRIMER_LEFT_24_TM=59.284
PRIMER_RIGHT_24_TM=60.569
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.7000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=645
PRIMER_PAIR_25_PENALTY=1.319286
PRIMER_LEFT_25_PENALTY=0.716126
PRIMER_RIGHT_25_PENALTY=0.603160
PRIMER_LEFT_25_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_25_SEQUENCE=CATTCCTTCAATCCGCAGTC
PRIMER_LEFT_25=715,20
PRIMER_RIGHT_25=1608,20
PRIMER_LEFT_25_TM=59.284
PRIMER_RIGHT_25_TM=60.603
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.7000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=894
PRIMER_PAIR_26_PENALTY=1.382701
PRIMER_LEFT_26_PENALTY=0.912701
PRIMER_RIGHT_26_PENALTY=0.470001
PRIMER_LEFT_26_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_26_SEQUENCE=CCTCCAGTCACCGTATCTCC
PRIMER_LEFT_26=716,20
PRIMER_RIGHT_26=1270,20
PRIMER_LEFT_26_TM=60.913
PRIMER_RIGHT_26_TM=59.530
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=60.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=555
PRIMER_PAIR_27_PENALTY=1.396638
PRIMER_LEFT_27_PENALTY=0.195358
PRIMER_RIGHT_27_PENALTY=1.201280
PRIMER_LEFT_27_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_27_SEQUENCE=CCGAACTAGACTAAGCAGCGA
PRIMER_LEFT_27=714,20
PRIMER_RIGHT_27=1427,21
PRIMER_LEFT_27_TM=60.195
PRIMER_RIGHT_27_TM=59.799
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=9.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=714
PRIMER_PAIR_28_PENALTY=1.398183
PRIMER_LEFT_28_PENALTY=0.196903
PRIMER_RIGHT_28_PENALTY=1.201280
PRIMER_LEFT_28_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_28_SEQUENCE=CCGAACTAGACTAAGCAGCGA
PRIMER_LEFT_28=713,20
PRIMER_RIGHT_28=1427,21
PRIMER_LEFT_28_TM=59.803
PRIMER_RIGHT_28_TM=59.799
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.1000
PRIMER_RIGHT_28_END_STABILITY=9.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=715
PRIMER_PAIR_29_PENALTY=1.409974
PRIMER_LEFT_29_PENALTY=0.716126
PRIMER_RIGHT_29_PENALTY=0.693848
PRIMER_LEFT_29_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_29_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_29=715,20
PRIMER_RIGHT_29=1358,20
PRIMER_LEFT_29_TM=59.284
PRIMER_RIGHT_29_TM=60.694
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=644
PRIMER_PAIR_30_PENALTY=1.482051
PRIMER_LEFT_30_PENALTY=0.912701
PRIMER_RIGHT_30_PENALTY=0.569350
PRIMER_LEFT_30_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_30_SEQUENCE=GTAGCTCCTCCCCCAAAAGA
PRIMER_LEFT_30=716,20
PRIMER_RIGHT_30=1359,20
PRIMER_LEFT_30_TM=60.913
PRIMER_RIGHT_30_TM=60.569
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=644
PRIMER_PAIR_31_PENALTY=1.491844
PRIMER_LEFT_31_PENALTY=0.716126
PRIMER_RIGHT_31_PENALTY=0.775718
PRIMER_LEFT_31_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_31_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_31=715,20
PRIMER_RIGHT_31=1599,20
PRIMER_LEFT_31_TM=59.284
PRIMER_RIGHT_31_TM=60.776
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.7000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=885
PRIMER_PAIR_32_PENALTY=1.515860
PRIMER_LEFT_32_PENALTY=0.912701
PRIMER_RIGHT_32_PENALTY=0.603160
PRIMER_LEFT_32_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_32_SEQUENCE=CATTCCTTCAATCCGCAGTC
PRIMER_LEFT_32=716,20
PRIMER_RIGHT_32=1608,20
PRIMER_LEFT_32_TM=60.913
PRIMER_RIGHT_32_TM=60.603
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=893
PRIMER_PAIR_33_PENALTY=1.606549
PRIMER_LEFT_33_PENALTY=0.912701
PRIMER_RIGHT_33_PENALTY=0.693848
PRIMER_LEFT_33_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_33_SEQUENCE=TAGCTCCTCCCCCAAAAGAG
PRIMER_LEFT_33=716,20
PRIMER_RIGHT_33=1358,20
PRIMER_LEFT_33_TM=60.913
PRIMER_RIGHT_33_TM=60.694
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=643
PRIMER_PAIR_34_PENALTY=1.639911
PRIMER_LEFT_34_PENALTY=0.716126
PRIMER_RIGHT_34_PENALTY=0.923785
PRIMER_LEFT_34_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_34_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_34=715,20
PRIMER_RIGHT_34=1523,20
PRIMER_LEFT_34_TM=59.284
PRIMER_RIGHT_34_TM=60.924
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.7000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=809
PRIMER_PAIR_35_PENALTY=1.688419
PRIMER_LEFT_35_PENALTY=0.912701
PRIMER_RIGHT_35_PENALTY=0.775718
PRIMER_LEFT_35_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_35_SEQUENCE=AATCCGCAGTCGACAAGAAA
PRIMER_LEFT_35=716,20
PRIMER_RIGHT_35=1599,20
PRIMER_LEFT_35_TM=60.913
PRIMER_RIGHT_35_TM=60.776
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=884
PRIMER_PAIR_36_PENALTY=1.698388
PRIMER_LEFT_36_PENALTY=0.716126
PRIMER_RIGHT_36_PENALTY=0.982262
PRIMER_LEFT_36_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_36_SEQUENCE=GTGCCTACAGACCCATTCCT
PRIMER_LEFT_36=715,20
PRIMER_RIGHT_36=1521,20
PRIMER_LEFT_36_TM=59.284
PRIMER_RIGHT_36_TM=59.018
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.7000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=807
PRIMER_PAIR_37_PENALTY=1.728223
PRIMER_LEFT_37_PENALTY=0.195358
PRIMER_RIGHT_37_PENALTY=1.532866
PRIMER_LEFT_37_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_37_SEQUENCE=ACCTCAAGGGACTTCTGCTC
PRIMER_LEFT_37=714,20
PRIMER_RIGHT_37=1694,20
PRIMER_LEFT_37_TM=60.195
PRIMER_RIGHT_37_TM=58.467
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=981
PRIMER_PAIR_38_PENALTY=1.729769
PRIMER_LEFT_38_PENALTY=0.196903
PRIMER_RIGHT_38_PENALTY=1.532866
PRIMER_LEFT_38_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_38_SEQUENCE=ACCTCAAGGGACTTCTGCTC
PRIMER_LEFT_38=713,20
PRIMER_RIGHT_38=1694,20
PRIMER_LEFT_38_TM=59.803
PRIMER_RIGHT_38_TM=58.467
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=982
PRIMER_PAIR_39_PENALTY=1.836485
PRIMER_LEFT_39_PENALTY=0.912701
PRIMER_RIGHT_39_PENALTY=0.923785
PRIMER_LEFT_39_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_39_SEQUENCE=TGGTGCCTACAGACCCATTC
PRIMER_LEFT_39=716,20
PRIMER_RIGHT_39=1523,20
PRIMER_LEFT_39_TM=60.913
PRIMER_RIGHT_39_TM=60.924
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=808
PRIMER_PAIR_40_PENALTY=1.879162
PRIMER_LEFT_40_PENALTY=0.195358
PRIMER_RIGHT_40_PENALTY=1.683804
PRIMER_LEFT_40_SEQUENCE=GATCGAACTCAAGGGACGAA
PRIMER_RIGHT_40_SEQUENCE=GGTGCCTACAGACCCATTCC
PRIMER_LEFT_40=714,20
PRIMER_RIGHT_40=1522,20
PRIMER_LEFT_40_TM=60.195
PRIMER_RIGHT_40_TM=61.684
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=60.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.4000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=809
PRIMER_PAIR_41_PENALTY=1.880707
PRIMER_LEFT_41_PENALTY=0.196903
PRIMER_RIGHT_41_PENALTY=1.683804
PRIMER_LEFT_41_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_41_SEQUENCE=GGTGCCTACAGACCCATTCC
PRIMER_LEFT_41=713,20
PRIMER_RIGHT_41=1522,20
PRIMER_LEFT_41_TM=59.803
PRIMER_RIGHT_41_TM=61.684
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=60.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=810
PRIMER_PAIR_42_PENALTY=1.894962
PRIMER_LEFT_42_PENALTY=0.912701
PRIMER_RIGHT_42_PENALTY=0.982262
PRIMER_LEFT_42_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_42_SEQUENCE=GTGCCTACAGACCCATTCCT
PRIMER_LEFT_42=716,20
PRIMER_RIGHT_42=1521,20
PRIMER_LEFT_42_TM=60.913
PRIMER_RIGHT_42_TM=59.018
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=806
PRIMER_PAIR_43_PENALTY=1.917406
PRIMER_LEFT_43_PENALTY=0.716126
PRIMER_RIGHT_43_PENALTY=1.201280
PRIMER_LEFT_43_SEQUENCE=ATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_43_SEQUENCE=CCGAACTAGACTAAGCAGCGA
PRIMER_LEFT_43=715,20
PRIMER_RIGHT_43=1427,21
PRIMER_LEFT_43_TM=59.284
PRIMER_RIGHT_43_TM=59.799
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.7000
PRIMER_RIGHT_43_END_STABILITY=9.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=713
PRIMER_PAIR_44_PENALTY=1.947754
PRIMER_LEFT_44_PENALTY=0.196903
PRIMER_RIGHT_44_PENALTY=1.750851
PRIMER_LEFT_44_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_44_SEQUENCE=CCTACAGACCCATTCCTCACC
PRIMER_LEFT_44=713,20
PRIMER_RIGHT_44=1518,21
PRIMER_LEFT_44_TM=59.803
PRIMER_RIGHT_44_TM=60.751
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=57.143
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=806
PRIMER_PAIR_45_PENALTY=2.094780
PRIMER_LEFT_45_PENALTY=0.196903
PRIMER_RIGHT_45_PENALTY=1.897877
PRIMER_LEFT_45_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_45_SEQUENCE=GCCTACAGACCCATTCCTCAC
PRIMER_LEFT_45=713,20
PRIMER_RIGHT_45=1519,21
PRIMER_LEFT_45_TM=59.803
PRIMER_RIGHT_45_TM=60.898
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=57.143
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=807
PRIMER_PAIR_46_PENALTY=2.094780
PRIMER_LEFT_46_PENALTY=0.196903
PRIMER_RIGHT_46_PENALTY=1.897877
PRIMER_LEFT_46_SEQUENCE=AGATCGAACTCAAGGGACGA
PRIMER_RIGHT_46_SEQUENCE=GTGCCTACAGACCCATTCCTC
PRIMER_LEFT_46=713,20
PRIMER_RIGHT_46=1521,21
PRIMER_LEFT_46_TM=59.803
PRIMER_RIGHT_46_TM=60.898
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=57.143
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.1000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=809
PRIMER_PAIR_47_PENALTY=2.113980
PRIMER_LEFT_47_PENALTY=0.912701
PRIMER_RIGHT_47_PENALTY=1.201280
PRIMER_LEFT_47_SEQUENCE=TCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_47_SEQUENCE=CCGAACTAGACTAAGCAGCGA
PRIMER_LEFT_47=716,20
PRIMER_RIGHT_47=1427,21
PRIMER_LEFT_47_TM=60.913
PRIMER_RIGHT_47_TM=59.799
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=712
PRIMER_PAIR_48_PENALTY=2.148596
PRIMER_LEFT_48_PENALTY=2.138051
PRIMER_RIGHT_48_PENALTY=0.010545
PRIMER_LEFT_48_SEQUENCE=ATCGAACTCAAGGGACGAAGA
PRIMER_RIGHT_48_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_48=715,21
PRIMER_RIGHT_48=1524,20
PRIMER_LEFT_48_TM=61.138
PRIMER_RIGHT_48_TM=59.989
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=810
PRIMER_PAIR_49_PENALTY=2.148596
PRIMER_LEFT_49_PENALTY=2.138051
PRIMER_RIGHT_49_PENALTY=0.010545
PRIMER_LEFT_49_SEQUENCE=GATCGAACTCAAGGGACGAAG
PRIMER_RIGHT_49_SEQUENCE=CTGGTGCCTACAGACCCATT
PRIMER_LEFT_49=714,21
PRIMER_RIGHT_49=1524,20
PRIMER_LEFT_49_TM=61.138
PRIMER_RIGHT_49_TM=59.989
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.7000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=811
=
SEQUENCE_ID=MAGIC.105_4_9429237_9429250_po-0_kn-0_94020_7619_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=CAAAAAATGAAAGAGCANAANANNAAGACGCNATCANAAAACCTTGATAACAATACGTGTGTGAGAACAATCCATACCATTGAGACGAAGCTCGATNATCCTNTTAAANGCATGNGCACATTTNCTCAACGTACTCTTAAACAGCTCCTTCCTANTCAACCGAGCTCNATTGCTTTTCTCAAANACCTATATAAACAAGATTGAGAAATTCACAAATCTAANANAGACCTAATCNTATTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAACNAAAATCAACGAGAATCGNGAAGAAATTCACCGGATCGCTGGCGACAAGGCGGGCAATTCGATCGGAAACNTCAAAAGCGTGNCGGGAACCAGCCCAGACGATCTTCATATCCTCNACGTCGAACTCNGNTTTGTTTCTCTCATCGGCNAGGTGATCAATTCCTTCCATGATTCGTNATTCAGAANCNAGTAGCTAAACACTCAAAANTTTCTNGAGAAAACTCTCAGAGATGANAGNAAAAANNNANNNAAAAAACAGAGGGAGAAAANGAAGGACANTGCCTNNCTCTACCGATTTAATTGCAGAGTAGTTGGAAGAACAATTAAGCTTTTTATTTCGGAGATAAAAGAAGAGAAAGACAAATTGATTTANATATAGACTCNTTTTTTTCTTCTTTCTATAATTAATATATTTTGGGTTTATCTTTTTCTCAGATCCACTCTCCGACATAGATAAAACCCACGTGGAAAATAATAAATTTTGGATTATATATTCCTAATATAAGNTAATTAGTCAACGAATGTTGAAAACAAATTANGTTGTTTGNAAATAAAACNTCNCAAGTATGGAATAATGTGATTTNNNCCCCCTNATNTATATATTTGACTAANAAAAAAAGNATATCTACGTATAGAAAATTTTNTATGCTGTATAACAANNNNNNNTTGACAGACAAAAAGTNGTTGTATTTCAGTATTTNTGTACTAAANTNAAANTTNAAATATCGTNAANTCAGANGTGTNNNNANNNNNNNTTTCTTTTNCNCANANGTGTTTCCATATGAATNAGTNAATGCNTGCATNNGAAGAACATGTTGATAAAAAGAAGATAAAANNGTTAGATTGAAGATAAGATAATNTTTTGTTNTCNTNTGTTTAAAATANTATTTTNNNNNNNNTTTGCAGGTANAGTNGNGTTATGCNNATTTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNTCNGTCAAANGCATTTGCTTGTCAACTGTNTGGTAAAANTTGATTAANATTTGTGTTTCCNTCNANGTATAGTTAAGTAATTACACTTGTATANAANCGTATTATNCTNGTNAANANTAAAATGTTTGTCTTTTGAATTGANACAGTAATGTCNCTANANACCACAGAAAATTCNNCANGTGTATGAGTATGGTCCAAACACGTAAACGCCCATTCAAGTTCCACGGCTTTTAAACGTGTCTATCTTTGAGANTTTGACNNACTNATCANTTTCAAGTTCCATAANTTNCACTAATNAAACATTATGGTANGTAGAAGTTTGAACANTANTNTTTTCTTNCATTTCGNCACAAACATNAGACTAGGAAGTGGCGTATGTTAGCCTGAAACGCTCCTTNATATAGTCNCCGGCNANNATAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTATTCTTTTACACNTGAANANCAAANGATTTANCAAANGTGTAANNTGCCTNNNCNCACNCNNTNAAATTGGATTAAGATTCAAGANGAAATACCTTGGGGGACAAGTTTNGCTGCAACAACTCTCCAAGCATTTCACATTACAATCTGGATTGGGATCTAAGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.715715
PRIMER_LEFT_0_PENALTY=1.605323
PRIMER_RIGHT_0_PENALTY=0.110392
PRIMER_LEFT_0_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_0_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_0=712,21
PRIMER_RIGHT_0=1606,20
PRIMER_LEFT_0_TM=59.395
PRIMER_RIGHT_0_TM=60.110
PRIMER_LEFT_0_GC_PERCENT=52.381
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=895
PRIMER_PAIR_1_PENALTY=1.738180
PRIMER_LEFT_1_PENALTY=1.605323
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_1_SEQUENCE=GAATGGGCGTTTACGTGTTT
PRIMER_LEFT_1=712,21
PRIMER_RIGHT_1=1591,20
PRIMER_LEFT_1_TM=59.395
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=880
PRIMER_PAIR_2_PENALTY=2.139576
PRIMER_LEFT_2_PENALTY=1.605323
PRIMER_RIGHT_2_PENALTY=0.534252
PRIMER_LEFT_2_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_2_SEQUENCE=ACACGTTTAAAAGCCGTGGA
PRIMER_LEFT_2=712,21
PRIMER_RIGHT_2=1615,20
PRIMER_LEFT_2_TM=59.395
PRIMER_RIGHT_2_TM=60.534
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=904
PRIMER_PAIR_3_PENALTY=2.255873
PRIMER_LEFT_3_PENALTY=2.145481
PRIMER_RIGHT_3_PENALTY=0.110392
PRIMER_LEFT_3_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_3_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_3=713,20
PRIMER_RIGHT_3=1606,20
PRIMER_LEFT_3_TM=57.855
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=894
PRIMER_PAIR_4_PENALTY=2.278337
PRIMER_LEFT_4_PENALTY=2.145481
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_4_SEQUENCE=GAATGGGCGTTTACGTGTTT
PRIMER_LEFT_4=713,20
PRIMER_RIGHT_4=1591,20
PRIMER_LEFT_4_TM=57.855
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=879
PRIMER_PAIR_5_PENALTY=2.338415
PRIMER_LEFT_5_PENALTY=1.605323
PRIMER_RIGHT_5_PENALTY=0.733091
PRIMER_LEFT_5_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_5_SEQUENCE=CGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_5=712,21
PRIMER_RIGHT_5=1612,20
PRIMER_LEFT_5_TM=59.395
PRIMER_RIGHT_5_TM=59.267
PRIMER_LEFT_5_GC_PERCENT=52.381
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=901
PRIMER_PAIR_6_PENALTY=2.387375
PRIMER_LEFT_6_PENALTY=1.605323
PRIMER_RIGHT_6_PENALTY=0.782051
PRIMER_LEFT_6_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_6_SEQUENCE=AATGGGCGTTTACGTGTTTG
PRIMER_LEFT_6=712,21
PRIMER_RIGHT_6=1590,20
PRIMER_LEFT_6_TM=59.395
PRIMER_RIGHT_6_TM=60.782
PRIMER_LEFT_6_GC_PERCENT=52.381
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=879
PRIMER_PAIR_7_PENALTY=2.621866
PRIMER_LEFT_7_PENALTY=1.605323
PRIMER_RIGHT_7_PENALTY=1.016543
PRIMER_LEFT_7_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_7_SEQUENCE=CACGTTTAAAAGCCGTGGAA
PRIMER_LEFT_7=712,21
PRIMER_RIGHT_7=1614,20
PRIMER_LEFT_7_TM=59.395
PRIMER_RIGHT_7_TM=61.017
PRIMER_LEFT_7_GC_PERCENT=52.381
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=903
PRIMER_PAIR_8_PENALTY=2.653735
PRIMER_LEFT_8_PENALTY=1.605323
PRIMER_RIGHT_8_PENALTY=1.048411
PRIMER_LEFT_8_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_8_SEQUENCE=ACGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_8=712,21
PRIMER_RIGHT_8=1613,21
PRIMER_LEFT_8_TM=59.395
PRIMER_RIGHT_8_TM=60.048
PRIMER_LEFT_8_GC_PERCENT=52.381
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=902
PRIMER_PAIR_9_PENALTY=2.679733
PRIMER_LEFT_9_PENALTY=2.145481
PRIMER_RIGHT_9_PENALTY=0.534252
PRIMER_LEFT_9_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_9_SEQUENCE=ACACGTTTAAAAGCCGTGGA
PRIMER_LEFT_9=713,20
PRIMER_RIGHT_9=1615,20
PRIMER_LEFT_9_TM=57.855
PRIMER_RIGHT_9_TM=60.534
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=903
PRIMER_PAIR_10_PENALTY=2.801955
PRIMER_LEFT_10_PENALTY=2.691563
PRIMER_RIGHT_10_PENALTY=0.110392
PRIMER_LEFT_10_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_10_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_10=715,22
PRIMER_RIGHT_10=1606,20
PRIMER_LEFT_10_TM=59.308
PRIMER_RIGHT_10_TM=60.110
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=892
PRIMER_PAIR_11_PENALTY=2.824420
PRIMER_LEFT_11_PENALTY=2.691563
PRIMER_RIGHT_11_PENALTY=0.132857
PRIMER_LEFT_11_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_11_SEQUENCE=GAATGGGCGTTTACGTGTTT
PRIMER_LEFT_11=715,22
PRIMER_RIGHT_11=1591,20
PRIMER_LEFT_11_TM=59.308
PRIMER_RIGHT_11_TM=59.867
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.9000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=877
PRIMER_PAIR_12_PENALTY=2.876425
PRIMER_LEFT_12_PENALTY=2.766033
PRIMER_RIGHT_12_PENALTY=0.110392
PRIMER_LEFT_12_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_12_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_12=711,22
PRIMER_RIGHT_12=1606,20
PRIMER_LEFT_12_TM=60.766
PRIMER_RIGHT_12_TM=60.110
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=896
PRIMER_PAIR_13_PENALTY=2.878572
PRIMER_LEFT_13_PENALTY=2.145481
PRIMER_RIGHT_13_PENALTY=0.733091
PRIMER_LEFT_13_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_13_SEQUENCE=CGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_13=713,20
PRIMER_RIGHT_13=1612,20
PRIMER_LEFT_13_TM=57.855
PRIMER_RIGHT_13_TM=59.267
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=900
PRIMER_PAIR_14_PENALTY=2.898890
PRIMER_LEFT_14_PENALTY=2.766033
PRIMER_RIGHT_14_PENALTY=0.132857
PRIMER_LEFT_14_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_14_SEQUENCE=GAATGGGCGTTTACGTGTTT
PRIMER_LEFT_14=711,22
PRIMER_RIGHT_14=1591,20
PRIMER_LEFT_14_TM=60.766
PRIMER_RIGHT_14_TM=59.867
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=881
PRIMER_PAIR_15_PENALTY=2.916256
PRIMER_LEFT_15_PENALTY=1.605323
PRIMER_RIGHT_15_PENALTY=1.310933
PRIMER_LEFT_15_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_15_SEQUENCE=TGAATGGGCGTTTACGTGTT
PRIMER_LEFT_15=712,21
PRIMER_RIGHT_15=1592,20
PRIMER_LEFT_15_TM=59.395
PRIMER_RIGHT_15_TM=61.311
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=881
PRIMER_PAIR_16_PENALTY=2.927532
PRIMER_LEFT_16_PENALTY=2.145481
PRIMER_RIGHT_16_PENALTY=0.782051
PRIMER_LEFT_16_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_16_SEQUENCE=AATGGGCGTTTACGTGTTTG
PRIMER_LEFT_16=713,20
PRIMER_RIGHT_16=1590,20
PRIMER_LEFT_16_TM=57.855
PRIMER_RIGHT_16_TM=60.782
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=878
PRIMER_PAIR_17_PENALTY=3.162023
PRIMER_LEFT_17_PENALTY=2.145481
PRIMER_RIGHT_17_PENALTY=1.016543
PRIMER_LEFT_17_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_17_SEQUENCE=CACGTTTAAAAGCCGTGGAA
PRIMER_LEFT_17=713,20
PRIMER_RIGHT_17=1614,20
PRIMER_LEFT_17_TM=57.855
PRIMER_RIGHT_17_TM=61.017
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=902
PRIMER_PAIR_18_PENALTY=3.193056
PRIMER_LEFT_18_PENALTY=1.605323
PRIMER_RIGHT_18_PENALTY=1.587733
PRIMER_LEFT_18_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_18_SEQUENCE=TTAAAAGCCGTGGAACTTGA
PRIMER_LEFT_18=712,21
PRIMER_RIGHT_18=1609,20
PRIMER_LEFT_18_TM=59.395
PRIMER_RIGHT_18_TM=58.412
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.6000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=898
PRIMER_PAIR_19_PENALTY=3.193892
PRIMER_LEFT_19_PENALTY=2.145481
PRIMER_RIGHT_19_PENALTY=1.048411
PRIMER_LEFT_19_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_19_SEQUENCE=ACGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_19=713,20
PRIMER_RIGHT_19=1613,21
PRIMER_LEFT_19_TM=57.855
PRIMER_RIGHT_19_TM=60.048
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=901
PRIMER_PAIR_20_PENALTY=3.225816
PRIMER_LEFT_20_PENALTY=2.691563
PRIMER_RIGHT_20_PENALTY=0.534252
PRIMER_LEFT_20_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_20_SEQUENCE=ACACGTTTAAAAGCCGTGGA
PRIMER_LEFT_20=715,22
PRIMER_RIGHT_20=1615,20
PRIMER_LEFT_20_TM=59.308
PRIMER_RIGHT_20_TM=60.534
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=5.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=901
PRIMER_PAIR_21_PENALTY=3.300286
PRIMER_LEFT_21_PENALTY=2.766033
PRIMER_RIGHT_21_PENALTY=0.534252
PRIMER_LEFT_21_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_21_SEQUENCE=ACACGTTTAAAAGCCGTGGA
PRIMER_LEFT_21=711,22
PRIMER_RIGHT_21=1615,20
PRIMER_LEFT_21_TM=60.766
PRIMER_RIGHT_21_TM=60.534
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=905
PRIMER_PAIR_22_PENALTY=3.384871
PRIMER_LEFT_22_PENALTY=3.274479
PRIMER_RIGHT_22_PENALTY=0.110392
PRIMER_LEFT_22_SEQUENCE=CTCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_22_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_22=714,23
PRIMER_RIGHT_22=1606,20
PRIMER_LEFT_22_TM=60.274
PRIMER_RIGHT_22_TM=60.110
PRIMER_LEFT_22_GC_PERCENT=52.174
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=5.9000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=893
PRIMER_PAIR_23_PENALTY=3.407335
PRIMER_LEFT_23_PENALTY=3.274479
PRIMER_RIGHT_23_PENALTY=0.132857
PRIMER_LEFT_23_SEQUENCE=CTCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_23_SEQUENCE=GAATGGGCGTTTACGTGTTT
PRIMER_LEFT_23=714,23
PRIMER_RIGHT_23=1591,20
PRIMER_LEFT_23_TM=60.274
PRIMER_RIGHT_23_TM=59.867
PRIMER_LEFT_23_GC_PERCENT=52.174
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=5.9000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=878
PRIMER_PAIR_24_PENALTY=3.424654
PRIMER_LEFT_24_PENALTY=2.691563
PRIMER_RIGHT_24_PENALTY=0.733091
PRIMER_LEFT_24_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_24_SEQUENCE=CGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_24=715,22
PRIMER_RIGHT_24=1612,20
PRIMER_LEFT_24_TM=59.308
PRIMER_RIGHT_24_TM=59.267
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=5.9000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=898
PRIMER_PAIR_25_PENALTY=3.456413
PRIMER_LEFT_25_PENALTY=2.145481
PRIMER_RIGHT_25_PENALTY=1.310933
PRIMER_LEFT_25_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_25_SEQUENCE=TGAATGGGCGTTTACGTGTT
PRIMER_LEFT_25=713,20
PRIMER_RIGHT_25=1592,20
PRIMER_LEFT_25_TM=57.855
PRIMER_RIGHT_25_TM=61.311
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=880
PRIMER_PAIR_26_PENALTY=3.473615
PRIMER_LEFT_26_PENALTY=2.691563
PRIMER_RIGHT_26_PENALTY=0.782051
PRIMER_LEFT_26_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_26_SEQUENCE=AATGGGCGTTTACGTGTTTG
PRIMER_LEFT_26=715,22
PRIMER_RIGHT_26=1590,20
PRIMER_LEFT_26_TM=59.308
PRIMER_RIGHT_26_TM=60.782
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=5.9000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=876
PRIMER_PAIR_27_PENALTY=3.499125
PRIMER_LEFT_27_PENALTY=2.766033
PRIMER_RIGHT_27_PENALTY=0.733091
PRIMER_LEFT_27_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_27_SEQUENCE=CGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_27=711,22
PRIMER_RIGHT_27=1612,20
PRIMER_LEFT_27_TM=60.766
PRIMER_RIGHT_27_TM=59.267
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.6000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=902
PRIMER_PAIR_28_PENALTY=3.529914
PRIMER_LEFT_28_PENALTY=3.419522
PRIMER_RIGHT_28_PENALTY=0.110392
PRIMER_LEFT_28_SEQUENCE=TCTCCGACATAGATAAAACCCA
PRIMER_RIGHT_28_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_28=725,22
PRIMER_RIGHT_28=1606,20
PRIMER_LEFT_28_TM=58.580
PRIMER_RIGHT_28_TM=60.110
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=882
PRIMER_PAIR_29_PENALTY=3.548085
PRIMER_LEFT_29_PENALTY=2.766033
PRIMER_RIGHT_29_PENALTY=0.782051
PRIMER_LEFT_29_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_29_SEQUENCE=AATGGGCGTTTACGTGTTTG
PRIMER_LEFT_29=711,22
PRIMER_RIGHT_29=1590,20
PRIMER_LEFT_29_TM=60.766
PRIMER_RIGHT_29_TM=60.782
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.6000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=880
PRIMER_PAIR_30_PENALTY=3.600378
PRIMER_LEFT_30_PENALTY=3.489986
PRIMER_RIGHT_30_PENALTY=0.110392
PRIMER_LEFT_30_SEQUENCE=CTCTCCGACATAGATAAAACCCA
PRIMER_RIGHT_30_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_30=724,23
PRIMER_RIGHT_30=1606,20
PRIMER_LEFT_30_TM=59.510
PRIMER_RIGHT_30_TM=60.110
PRIMER_LEFT_30_GC_PERCENT=43.478
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=883
PRIMER_PAIR_31_PENALTY=3.650521
PRIMER_LEFT_31_PENALTY=1.605323
PRIMER_RIGHT_31_PENALTY=2.045198
PRIMER_LEFT_31_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_31_SEQUENCE=TTTAAAAGCCGTGGAACTTG
PRIMER_LEFT_31=712,21
PRIMER_RIGHT_31=1610,20
PRIMER_LEFT_31_TM=59.395
PRIMER_RIGHT_31_TM=57.955
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=899
PRIMER_PAIR_32_PENALTY=3.708106
PRIMER_LEFT_32_PENALTY=2.691563
PRIMER_RIGHT_32_PENALTY=1.016543
PRIMER_LEFT_32_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_32_SEQUENCE=CACGTTTAAAAGCCGTGGAA
PRIMER_LEFT_32=715,22
PRIMER_RIGHT_32=1614,20
PRIMER_LEFT_32_TM=59.308
PRIMER_RIGHT_32_TM=61.017
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=5.9000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=900
PRIMER_PAIR_33_PENALTY=3.725207
PRIMER_LEFT_33_PENALTY=3.614815
PRIMER_RIGHT_33_PENALTY=0.110392
PRIMER_LEFT_33_SEQUENCE=TCTCCGACATAGATAAAACCCAC
PRIMER_RIGHT_33_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_33=725,23
PRIMER_RIGHT_33=1606,20
PRIMER_LEFT_33_TM=59.385
PRIMER_RIGHT_33_TM=60.110
PRIMER_LEFT_33_GC_PERCENT=43.478
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=882
PRIMER_PAIR_34_PENALTY=3.733213
PRIMER_LEFT_34_PENALTY=2.145481
PRIMER_RIGHT_34_PENALTY=1.587733
PRIMER_LEFT_34_SEQUENCE=TCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_34_SEQUENCE=TTAAAAGCCGTGGAACTTGA
PRIMER_LEFT_34=713,20
PRIMER_RIGHT_34=1609,20
PRIMER_LEFT_34_TM=57.855
PRIMER_RIGHT_34_TM=58.412
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.6000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=897
PRIMER_PAIR_35_PENALTY=3.739975
PRIMER_LEFT_35_PENALTY=2.691563
PRIMER_RIGHT_35_PENALTY=1.048411
PRIMER_LEFT_35_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_35_SEQUENCE=ACGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_35=715,22
PRIMER_RIGHT_35=1613,21
PRIMER_LEFT_35_TM=59.308
PRIMER_RIGHT_35_TM=60.048
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=5.9000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=899
PRIMER_PAIR_36_PENALTY=3.747672
PRIMER_LEFT_36_PENALTY=3.614815
PRIMER_RIGHT_36_PENALTY=0.132857
PRIMER_LEFT_36_SEQUENCE=TCTCCGACATAGATAAAACCCAC
PRIMER_RIGHT_36_SEQUENCE=GAATGGGCGTTTACGTGTTT
PRIMER_LEFT_36=725,23
PRIMER_RIGHT_36=1591,20
PRIMER_LEFT_36_TM=59.385
PRIMER_RIGHT_36_TM=59.867
PRIMER_LEFT_36_GC_PERCENT=43.478
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=867
PRIMER_PAIR_37_PENALTY=3.770594
PRIMER_LEFT_37_PENALTY=3.660202
PRIMER_RIGHT_37_PENALTY=0.110392
PRIMER_LEFT_37_SEQUENCE=CAGATCCACTCTCCGACATAG
PRIMER_RIGHT_37_SEQUENCE=AAAGCCGTGGAACTTGAATG
PRIMER_LEFT_37=716,21
PRIMER_RIGHT_37=1606,20
PRIMER_LEFT_37_TM=57.340
PRIMER_RIGHT_37_TM=60.110
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=891
PRIMER_PAIR_38_PENALTY=3.782576
PRIMER_LEFT_38_PENALTY=2.766033
PRIMER_RIGHT_38_PENALTY=1.016543
PRIMER_LEFT_38_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_38_SEQUENCE=CACGTTTAAAAGCCGTGGAA
PRIMER_LEFT_38=711,22
PRIMER_RIGHT_38=1614,20
PRIMER_LEFT_38_TM=60.766
PRIMER_RIGHT_38_TM=61.017
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=904
PRIMER_PAIR_39_PENALTY=3.793059
PRIMER_LEFT_39_PENALTY=3.660202
PRIMER_RIGHT_39_PENALTY=0.132857
PRIMER_LEFT_39_SEQUENCE=CAGATCCACTCTCCGACATAG
PRIMER_RIGHT_39_SEQUENCE=GAATGGGCGTTTACGTGTTT
PRIMER_LEFT_39=716,21
PRIMER_RIGHT_39=1591,20
PRIMER_LEFT_39_TM=57.340
PRIMER_RIGHT_39_TM=59.867
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=5.9000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=876
PRIMER_PAIR_40_PENALTY=3.807396
PRIMER_LEFT_40_PENALTY=1.605323
PRIMER_RIGHT_40_PENALTY=2.202073
PRIMER_LEFT_40_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_40_SEQUENCE=GTTTAAAAGCCGTGGAACTTG
PRIMER_LEFT_40=712,21
PRIMER_RIGHT_40=1611,21
PRIMER_LEFT_40_TM=59.395
PRIMER_RIGHT_40_TM=58.798
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=900
PRIMER_PAIR_41_PENALTY=3.808731
PRIMER_LEFT_41_PENALTY=3.274479
PRIMER_RIGHT_41_PENALTY=0.534252
PRIMER_LEFT_41_SEQUENCE=CTCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_41_SEQUENCE=ACACGTTTAAAAGCCGTGGA
PRIMER_LEFT_41=714,23
PRIMER_RIGHT_41=1615,20
PRIMER_LEFT_41_TM=60.274
PRIMER_RIGHT_41_TM=60.534
PRIMER_LEFT_41_GC_PERCENT=52.174
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=5.9000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=902
PRIMER_PAIR_42_PENALTY=3.814445
PRIMER_LEFT_42_PENALTY=2.766033
PRIMER_RIGHT_42_PENALTY=1.048411
PRIMER_LEFT_42_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_42_SEQUENCE=ACGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_42=711,22
PRIMER_RIGHT_42=1613,21
PRIMER_LEFT_42_TM=60.766
PRIMER_RIGHT_42_TM=60.048
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.6000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=903
PRIMER_PAIR_43_PENALTY=3.953774
PRIMER_LEFT_43_PENALTY=3.419522
PRIMER_RIGHT_43_PENALTY=0.534252
PRIMER_LEFT_43_SEQUENCE=TCTCCGACATAGATAAAACCCA
PRIMER_RIGHT_43_SEQUENCE=ACACGTTTAAAAGCCGTGGA
PRIMER_LEFT_43=725,22
PRIMER_RIGHT_43=1615,20
PRIMER_LEFT_43_TM=58.580
PRIMER_RIGHT_43_TM=60.534
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=891
PRIMER_PAIR_44_PENALTY=3.971845
PRIMER_LEFT_44_PENALTY=1.605323
PRIMER_RIGHT_44_PENALTY=2.366522
PRIMER_LEFT_44_SEQUENCE=TTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_44_SEQUENCE=AAAAGCCGTGGAACTTGAATG
PRIMER_LEFT_44=712,21
PRIMER_RIGHT_44=1607,21
PRIMER_LEFT_44_TM=59.395
PRIMER_RIGHT_44_TM=61.367
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=896
PRIMER_PAIR_45_PENALTY=4.002496
PRIMER_LEFT_45_PENALTY=2.691563
PRIMER_RIGHT_45_PENALTY=1.310933
PRIMER_LEFT_45_SEQUENCE=TCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_45_SEQUENCE=TGAATGGGCGTTTACGTGTT
PRIMER_LEFT_45=715,22
PRIMER_RIGHT_45=1592,20
PRIMER_LEFT_45_TM=59.308
PRIMER_RIGHT_45_TM=61.311
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=5.9000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=878
PRIMER_PAIR_46_PENALTY=4.007570
PRIMER_LEFT_46_PENALTY=3.274479
PRIMER_RIGHT_46_PENALTY=0.733091
PRIMER_LEFT_46_SEQUENCE=CTCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_46_SEQUENCE=CGTTTAAAAGCCGTGGAACT
PRIMER_LEFT_46=714,23
PRIMER_RIGHT_46=1612,20
PRIMER_LEFT_46_TM=60.274
PRIMER_RIGHT_46_TM=59.267
PRIMER_LEFT_46_GC_PERCENT=52.174
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=5.9000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=899
PRIMER_PAIR_47_PENALTY=4.024238
PRIMER_LEFT_47_PENALTY=3.489986
PRIMER_RIGHT_47_PENALTY=0.534252
PRIMER_LEFT_47_SEQUENCE=CTCTCCGACATAGATAAAACCCA
PRIMER_RIGHT_47_SEQUENCE=ACACGTTTAAAAGCCGTGGA
PRIMER_LEFT_47=724,23
PRIMER_RIGHT_47=1615,20
PRIMER_LEFT_47_TM=59.510
PRIMER_RIGHT_47_TM=60.534
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=892
PRIMER_PAIR_48_PENALTY=4.056530
PRIMER_LEFT_48_PENALTY=3.274479
PRIMER_RIGHT_48_PENALTY=0.782051
PRIMER_LEFT_48_SEQUENCE=CTCAGATCCACTCTCCGACATAG
PRIMER_RIGHT_48_SEQUENCE=AATGGGCGTTTACGTGTTTG
PRIMER_LEFT_48=714,23
PRIMER_RIGHT_48=1590,20
PRIMER_LEFT_48_TM=60.274
PRIMER_RIGHT_48_TM=60.782
PRIMER_LEFT_48_GC_PERCENT=52.174
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.9000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=877
PRIMER_PAIR_49_PENALTY=4.076966
PRIMER_LEFT_49_PENALTY=2.766033
PRIMER_RIGHT_49_PENALTY=1.310933
PRIMER_LEFT_49_SEQUENCE=TTTCTCAGATCCACTCTCCGAC
PRIMER_RIGHT_49_SEQUENCE=TGAATGGGCGTTTACGTGTT
PRIMER_LEFT_49=711,22
PRIMER_RIGHT_49=1592,20
PRIMER_LEFT_49_TM=60.766
PRIMER_RIGHT_49_TM=61.311
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=882
=
SEQUENCE_ID=MAGIC.338_2_6666253_6666263_rsch-4_po-0_12341_31532_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=TGCGTAATTGCCCTTTTGTGCTTCATCAAAATAATANAATGGCNGACAAAACAAAAGCAAAATCGTTTCACGTAGTCATGGAAGCTATAACGGACGGTTCTGCATGTACATGGAAGCTATCTATTGTAATTGCTTATGTTGTAGTTAGTCTATCATCATANNNNNNNNNNNNNNNNNNNNNNNNTGATGANATAATATATGNCNAAAAAAATCAAAGAATACCTATAATTATTATTTTTGTTTTGCTTTGAANGAGCCAAAATAGTTTNTTTTTTNNCCAAAATCAGAGANNCTCAAGAGGGTCGATGTCAGANGAAGACTTGGTTTATACAAAATNACTTTGTTTTTAGACATCTATCGNTTAATTAANNATATNCATATGGTACAANNACTTGTTTAGAGATTCTCAANACTGCCTGGTCCACTAGAACTGGATAGTNTAAAATACACATATGTTTGATTAAGAAATTNAATATTTCATTTTATTAATNCNNTTTCANTTAGTTTATNNTNTNANGTTGCANTAGAATNATCNGTNAAGTTTAAATTTNNGTCATTNTANAGNCATTNATTNATATTTTGCTTTCANCCACAACTCATTTANNTTTTAAATNGTTCNANCTGTCNAAGNANACAAAANTNTCTNNANANCANATTGNTTTTTATTTANCATTCANNANCTATANTTNGTAATNGTGNANATCNAAAAAAATAAAAGAAAANCGNNNNNNNNNNNCTGCGAACCNTNTTTAAACNAANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNGACTNGACNNCAATTAGGNTTTGAATNNATNTTGTGGTTAGTGCNNACTAATCCNTNANCNGTCGANTCNATANNNNNNNNNNNNNNNNNNNNNNNNNCNANNAATTANTTTAAANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTNCGCACNANNATATANTATATATAANTTACNAATANTAAATAAAAANNNATNNNATCTATTGANNGTNCTNTNCNNNNAGANCNNNGTAACCATTCNAANCNTTANTATACTANCTAATTTAAATCTCAATAAATATATAAAANCACACAATNCAACNAAATAAATACTATAAGTTCCGNTCCAATNTTTGTAANTTTATTTGTGTACATACGAAGATCTCGANGAATATAGATATTAGTATTTCTCCAAGACTATAGAGTAAACTCATGACTAAAAGCATTATTTTACAAAATCACTGACACAAAAAGTCACCGACACCGACAAAACTAAGACTTTGGTTTTCCACCATAAGCTTGCTTACAAATGTAAAATGGTTTCTGCGATGGAGCACACATCTTAGCTTTCCCTGGACATCCCTGTAGACAATTATTCCCCAAACTTCCCATTACTTCTGCGTCTNTTCTCACAAATCTNGCCGGAGGTTTTCCCGTCAAGAAGTTCCCGTCTAAGTAAAGCCGTCTTAATGTCTTGCTCCGTTCGTATTCCGACGGTATAACACCGTGAAGCACGTTGTATCTCATAGANAGTGAAGAAAGTCTTGGATACGCCGCGAAACTCGCCGGAGCGTTTCCTCTGATTTGGTTGAATCCTAGCTCTACAGCTACGAGGTTGTTTTCTCCGGCGAGGTTTGGAGGGAGGACTTCGATGCCTGTGAGAGTGTTGTTCGCTAGATCGACTTGTTGGATTGATTCAAGTAGGAAGAACCAAGCTCCGAGTGTTCCGGTGAATGAGTTCTCAGCGANTTCNACGATTTCGAGCTGAGTTGACTCGGTGAATGAGTCTTTTGAGATGGGTCCTGATAAAGTATTCGCCTTTAAAGCTAAATCGATGAGGTTTTTCGGAAGCTTTGGGATGGCTCCGGTGAGCTTGTTGTAGCTAAGATCGAGTTGTCGGAGATTTGAAAGCGAGTTCATTGTTTTTGGAAGCGGCCCGGTGAGAGAGTTGTGAGATATGTCAATGGATTCGAGTGAGTTGAGTCGAGTTACTGAGTCGGGTAATGAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.634656
PRIMER_LEFT_0_PENALTY=1.622186
PRIMER_RIGHT_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_0_SEQUENCE=GTCGGTGTCGGTGACTTTTT
PRIMER_LEFT_0=292,20
PRIMER_RIGHT_0=1269,20
PRIMER_LEFT_0_TM=58.378
PRIMER_RIGHT_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=978
PRIMER_PAIR_1_PENALTY=2.012799
PRIMER_LEFT_1_PENALTY=1.622186
PRIMER_RIGHT_1_PENALTY=0.390614
PRIMER_LEFT_1_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_1_SEQUENCE=TAGTTTTGTCGGTGTCGGTG
PRIMER_LEFT_1=292,20
PRIMER_RIGHT_1=1276,20
PRIMER_LEFT_1_TM=58.378
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=985
PRIMER_PAIR_2_PENALTY=2.588655
PRIMER_LEFT_2_PENALTY=1.622186
PRIMER_RIGHT_2_PENALTY=0.966469
PRIMER_LEFT_2_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_2_SEQUENCE=GGTGTCGGTGACTTTTTGTG
PRIMER_LEFT_2=292,20
PRIMER_RIGHT_2=1266,20
PRIMER_LEFT_2_TM=58.378
PRIMER_RIGHT_2_TM=59.034
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=975
PRIMER_PAIR_3_PENALTY=2.606633
PRIMER_LEFT_3_PENALTY=1.622186
PRIMER_RIGHT_3_PENALTY=0.984448
PRIMER_LEFT_3_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_3_SEQUENCE=CGGTGTCGGTGACTTTTTGT
PRIMER_LEFT_3=292,20
PRIMER_RIGHT_3=1267,20
PRIMER_LEFT_3_TM=58.378
PRIMER_RIGHT_3_TM=60.984
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=976
PRIMER_PAIR_4_PENALTY=2.700003
PRIMER_LEFT_4_PENALTY=1.622186
PRIMER_RIGHT_4_PENALTY=1.077818
PRIMER_LEFT_4_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_4_SEQUENCE=GGTGTCGGTGACTTTTTGTGT
PRIMER_LEFT_4=292,20
PRIMER_RIGHT_4=1266,21
PRIMER_LEFT_4_TM=58.378
PRIMER_RIGHT_4_TM=59.922
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=975
PRIMER_PAIR_5_PENALTY=2.775774
PRIMER_LEFT_5_PENALTY=1.622186
PRIMER_RIGHT_5_PENALTY=1.153589
PRIMER_LEFT_5_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_5_SEQUENCE=TCTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_5=292,20
PRIMER_RIGHT_5=1279,21
PRIMER_LEFT_5_TM=58.378
PRIMER_RIGHT_5_TM=60.154
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=988
PRIMER_PAIR_6_PENALTY=3.174349
PRIMER_LEFT_6_PENALTY=1.622186
PRIMER_RIGHT_6_PENALTY=1.552164
PRIMER_LEFT_6_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_6_SEQUENCE=TGTCGGTGTCGGTGACTTTT
PRIMER_LEFT_6=292,20
PRIMER_RIGHT_6=1270,20
PRIMER_LEFT_6_TM=58.378
PRIMER_RIGHT_6_TM=61.552
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=979
PRIMER_PAIR_7_PENALTY=3.174349
PRIMER_LEFT_7_PENALTY=1.622186
PRIMER_RIGHT_7_PENALTY=1.552164
PRIMER_LEFT_7_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_7_SEQUENCE=TTGTCGGTGTCGGTGACTTT
PRIMER_LEFT_7=292,20
PRIMER_RIGHT_7=1271,20
PRIMER_LEFT_7_TM=58.378
PRIMER_RIGHT_7_TM=61.552
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=980
PRIMER_PAIR_8_PENALTY=3.174349
PRIMER_LEFT_8_PENALTY=1.622186
PRIMER_RIGHT_8_PENALTY=1.552164
PRIMER_LEFT_8_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_8_SEQUENCE=TTTGTCGGTGTCGGTGACTT
PRIMER_LEFT_8=292,20
PRIMER_RIGHT_8=1272,20
PRIMER_LEFT_8_TM=58.378
PRIMER_RIGHT_8_TM=61.552
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=981
PRIMER_PAIR_9_PENALTY=3.174349
PRIMER_LEFT_9_PENALTY=1.622186
PRIMER_RIGHT_9_PENALTY=1.552164
PRIMER_LEFT_9_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_9_SEQUENCE=TTTTGTCGGTGTCGGTGACT
PRIMER_LEFT_9=292,20
PRIMER_RIGHT_9=1273,20
PRIMER_LEFT_9_TM=58.378
PRIMER_RIGHT_9_TM=61.552
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=982
PRIMER_PAIR_10_PENALTY=3.174349
PRIMER_LEFT_10_PENALTY=1.622186
PRIMER_RIGHT_10_PENALTY=1.552164
PRIMER_LEFT_10_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_10_SEQUENCE=AGTTTTGTCGGTGTCGGTGA
PRIMER_LEFT_10=292,20
PRIMER_RIGHT_10=1275,20
PRIMER_LEFT_10_TM=58.378
PRIMER_RIGHT_10_TM=61.552
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=984
PRIMER_PAIR_11_PENALTY=3.339794
PRIMER_LEFT_11_PENALTY=1.622186
PRIMER_RIGHT_11_PENALTY=1.717608
PRIMER_LEFT_11_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_11_SEQUENCE=CTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_11=292,20
PRIMER_RIGHT_11=1278,20
PRIMER_LEFT_11_TM=58.378
PRIMER_RIGHT_11_TM=58.282
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=987
PRIMER_PAIR_12_PENALTY=3.466515
PRIMER_LEFT_12_PENALTY=1.622186
PRIMER_RIGHT_12_PENALTY=1.844329
PRIMER_LEFT_12_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_12_SEQUENCE=CTTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_12=292,20
PRIMER_RIGHT_12=1278,21
PRIMER_LEFT_12_TM=58.378
PRIMER_RIGHT_12_TM=59.156
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=987
PRIMER_PAIR_13_PENALTY=3.489987
PRIMER_LEFT_13_PENALTY=1.622186
PRIMER_RIGHT_13_PENALTY=1.867802
PRIMER_LEFT_13_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_13_SEQUENCE=TTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_13=292,20
PRIMER_RIGHT_13=1277,20
PRIMER_LEFT_13_TM=58.378
PRIMER_RIGHT_13_TM=58.132
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=986
PRIMER_PAIR_14_PENALTY=3.579773
PRIMER_LEFT_14_PENALTY=1.622186
PRIMER_RIGHT_14_PENALTY=1.957587
PRIMER_LEFT_14_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_14_SEQUENCE=TTAGTTTTGTCGGTGTCGGTG
PRIMER_LEFT_14=292,20
PRIMER_RIGHT_14=1277,21
PRIMER_LEFT_14_TM=58.378
PRIMER_RIGHT_14_TM=60.958
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=986
PRIMER_PAIR_15_PENALTY=3.679850
PRIMER_LEFT_15_PENALTY=1.622186
PRIMER_RIGHT_15_PENALTY=2.057665
PRIMER_LEFT_15_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_15_SEQUENCE=TCGGTGTCGGTGACTTTTTG
PRIMER_LEFT_15=292,20
PRIMER_RIGHT_15=1268,20
PRIMER_LEFT_15_TM=58.378
PRIMER_RIGHT_15_TM=62.058
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=977
PRIMER_PAIR_16_PENALTY=4.124582
PRIMER_LEFT_16_PENALTY=1.622186
PRIMER_RIGHT_16_PENALTY=2.502397
PRIMER_LEFT_16_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_16_SEQUENCE=TAGTTTTGTCGGTGTCGGTGA
PRIMER_LEFT_16=292,20
PRIMER_RIGHT_16=1276,21
PRIMER_LEFT_16_TM=58.378
PRIMER_RIGHT_16_TM=61.502
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=985
PRIMER_PAIR_17_PENALTY=4.346784
PRIMER_LEFT_17_PENALTY=1.622186
PRIMER_RIGHT_17_PENALTY=2.724598
PRIMER_LEFT_17_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_17_SEQUENCE=TCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_17=292,20
PRIMER_RIGHT_17=1262,20
PRIMER_LEFT_17_TM=58.378
PRIMER_RIGHT_17_TM=57.275
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=971
PRIMER_PAIR_18_PENALTY=4.364860
PRIMER_LEFT_18_PENALTY=1.622186
PRIMER_RIGHT_18_PENALTY=2.742674
PRIMER_LEFT_18_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_18_SEQUENCE=TCGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_18=292,20
PRIMER_RIGHT_18=1262,21
PRIMER_LEFT_18_TM=58.378
PRIMER_RIGHT_18_TM=58.257
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=971
PRIMER_PAIR_19_PENALTY=4.364860
PRIMER_LEFT_19_PENALTY=1.622186
PRIMER_RIGHT_19_PENALTY=2.742674
PRIMER_LEFT_19_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_19_SEQUENCE=GTCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_19=292,20
PRIMER_RIGHT_19=1263,21
PRIMER_LEFT_19_TM=58.378
PRIMER_RIGHT_19_TM=58.257
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=972
PRIMER_PAIR_20_PENALTY=4.461063
PRIMER_LEFT_20_PENALTY=1.622186
PRIMER_RIGHT_20_PENALTY=2.838878
PRIMER_LEFT_20_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_20_SEQUENCE=GTCGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_20=292,20
PRIMER_RIGHT_20=1263,22
PRIMER_LEFT_20_TM=58.378
PRIMER_RIGHT_20_TM=59.161
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=972
PRIMER_PAIR_21_PENALTY=4.517974
PRIMER_LEFT_21_PENALTY=1.622186
PRIMER_RIGHT_21_PENALTY=2.895789
PRIMER_LEFT_21_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_21_SEQUENCE=TGTCGGTGACTTTTTGTGTC
PRIMER_LEFT_21=292,20
PRIMER_RIGHT_21=1264,20
PRIMER_LEFT_21_TM=58.378
PRIMER_RIGHT_21_TM=57.104
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=6.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=973
PRIMER_PAIR_22_PENALTY=4.520597
PRIMER_LEFT_22_PENALTY=1.622186
PRIMER_RIGHT_22_PENALTY=2.898412
PRIMER_LEFT_22_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_22_SEQUENCE=GTGTCGGTGACTTTTTGTGTC
PRIMER_LEFT_22=292,20
PRIMER_RIGHT_22=1265,21
PRIMER_LEFT_22_TM=58.378
PRIMER_RIGHT_22_TM=58.102
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=974
PRIMER_PAIR_23_PENALTY=4.553685
PRIMER_LEFT_23_PENALTY=1.622186
PRIMER_RIGHT_23_PENALTY=2.931499
PRIMER_LEFT_23_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_23_SEQUENCE=TCTTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_23=292,20
PRIMER_RIGHT_23=1279,22
PRIMER_LEFT_23_TM=58.378
PRIMER_RIGHT_23_TM=60.931
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=4.553685
PRIMER_LEFT_24_PENALTY=1.622186
PRIMER_RIGHT_24_PENALTY=2.931499
PRIMER_LEFT_24_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_24_SEQUENCE=GTCTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_24=292,20
PRIMER_RIGHT_24=1280,22
PRIMER_LEFT_24_TM=58.378
PRIMER_RIGHT_24_TM=60.931
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=989
PRIMER_PAIR_25_PENALTY=4.697636
PRIMER_LEFT_25_PENALTY=1.622186
PRIMER_RIGHT_25_PENALTY=3.075450
PRIMER_LEFT_25_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_25_SEQUENCE=CCAAAGTCTTAGTTTTGTCGGTG
PRIMER_LEFT_25=292,20
PRIMER_RIGHT_25=1285,23
PRIMER_LEFT_25_TM=58.378
PRIMER_RIGHT_25_TM=60.075
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=994
PRIMER_PAIR_26_PENALTY=4.774664
PRIMER_LEFT_26_PENALTY=1.622186
PRIMER_RIGHT_26_PENALTY=3.152478
PRIMER_LEFT_26_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_26_SEQUENCE=TGTCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_26=292,20
PRIMER_RIGHT_26=1264,22
PRIMER_LEFT_26_TM=58.378
PRIMER_RIGHT_26_TM=61.152
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=973
PRIMER_PAIR_27_PENALTY=4.952976
PRIMER_LEFT_27_PENALTY=1.622186
PRIMER_RIGHT_27_PENALTY=3.330790
PRIMER_LEFT_27_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_27_SEQUENCE=GTTTTGTCGGTGTCGGTGACT
PRIMER_LEFT_27=292,20
PRIMER_RIGHT_27=1274,21
PRIMER_LEFT_27_TM=58.378
PRIMER_RIGHT_27_TM=62.331
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=983
PRIMER_PAIR_28_PENALTY=5.386985
PRIMER_LEFT_28_PENALTY=1.622186
PRIMER_RIGHT_28_PENALTY=3.764799
PRIMER_LEFT_28_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_28_SEQUENCE=GGTGTCGGTGACTTTTTGTGTC
PRIMER_LEFT_28=292,20
PRIMER_RIGHT_28=1266,22
PRIMER_LEFT_28_TM=58.378
PRIMER_RIGHT_28_TM=61.765
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=975
PRIMER_PAIR_29_PENALTY=5.426619
PRIMER_LEFT_29_PENALTY=1.622186
PRIMER_RIGHT_29_PENALTY=3.804433
PRIMER_LEFT_29_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_29_SEQUENCE=TCGGTGTCGGTGACTTTTTGT
PRIMER_LEFT_29=292,20
PRIMER_RIGHT_29=1268,21
PRIMER_LEFT_29_TM=58.378
PRIMER_RIGHT_29_TM=62.804
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=977
PRIMER_PAIR_30_PENALTY=5.426619
PRIMER_LEFT_30_PENALTY=1.622186
PRIMER_RIGHT_30_PENALTY=3.804433
PRIMER_LEFT_30_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_30_SEQUENCE=GTCGGTGTCGGTGACTTTTTG
PRIMER_LEFT_30=292,20
PRIMER_RIGHT_30=1269,21
PRIMER_LEFT_30_TM=58.378
PRIMER_RIGHT_30_TM=62.804
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=978
PRIMER_PAIR_31_PENALTY=5.426619
PRIMER_LEFT_31_PENALTY=1.622186
PRIMER_RIGHT_31_PENALTY=3.804433
PRIMER_LEFT_31_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_31_SEQUENCE=TGTCGGTGTCGGTGACTTTTT
PRIMER_LEFT_31=292,20
PRIMER_RIGHT_31=1270,21
PRIMER_LEFT_31_TM=58.378
PRIMER_RIGHT_31_TM=62.804
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=5.426619
PRIMER_LEFT_32_PENALTY=1.622186
PRIMER_RIGHT_32_PENALTY=3.804433
PRIMER_LEFT_32_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_32_SEQUENCE=TTGTCGGTGTCGGTGACTTTT
PRIMER_LEFT_32=292,20
PRIMER_RIGHT_32=1271,21
PRIMER_LEFT_32_TM=58.378
PRIMER_RIGHT_32_TM=62.804
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=980
PRIMER_PAIR_33_PENALTY=5.426619
PRIMER_LEFT_33_PENALTY=1.622186
PRIMER_RIGHT_33_PENALTY=3.804433
PRIMER_LEFT_33_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_33_SEQUENCE=TTTGTCGGTGTCGGTGACTTT
PRIMER_LEFT_33=292,20
PRIMER_RIGHT_33=1272,21
PRIMER_LEFT_33_TM=58.378
PRIMER_RIGHT_33_TM=62.804
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=981
PRIMER_PAIR_34_PENALTY=5.426619
PRIMER_LEFT_34_PENALTY=1.622186
PRIMER_RIGHT_34_PENALTY=3.804433
PRIMER_LEFT_34_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_34_SEQUENCE=TTTTGTCGGTGTCGGTGACTT
PRIMER_LEFT_34=292,20
PRIMER_RIGHT_34=1273,21
PRIMER_LEFT_34_TM=58.378
PRIMER_RIGHT_34_TM=62.804
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=982
PRIMER_PAIR_35_PENALTY=5.430007
PRIMER_LEFT_35_PENALTY=1.622186
PRIMER_RIGHT_35_PENALTY=3.807821
PRIMER_LEFT_35_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_35_SEQUENCE=CGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_35=292,20
PRIMER_RIGHT_35=1261,20
PRIMER_LEFT_35_TM=58.378
PRIMER_RIGHT_35_TM=56.192
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=970
PRIMER_PAIR_36_PENALTY=5.447618
PRIMER_LEFT_36_PENALTY=1.622186
PRIMER_RIGHT_36_PENALTY=3.825433
PRIMER_LEFT_36_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_36_SEQUENCE=CTTAGTTTTGTCGGTGTCGGTG
PRIMER_LEFT_36=292,20
PRIMER_RIGHT_36=1278,22
PRIMER_LEFT_36_TM=58.378
PRIMER_RIGHT_36_TM=61.825
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=9.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=987
PRIMER_PAIR_37_PENALTY=5.609038
PRIMER_LEFT_37_PENALTY=1.622186
PRIMER_RIGHT_37_PENALTY=3.986853
PRIMER_LEFT_37_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_37_SEQUENCE=GTGTCGGTGACTTTTTGTGT
PRIMER_LEFT_37=292,20
PRIMER_RIGHT_37=1265,20
PRIMER_LEFT_37_TM=58.378
PRIMER_RIGHT_37_TM=56.013
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=974
PRIMER_PAIR_38_PENALTY=6.130501
PRIMER_LEFT_38_PENALTY=1.622186
PRIMER_RIGHT_38_PENALTY=4.508316
PRIMER_LEFT_38_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_38_SEQUENCE=CCAAAGTCTTAGTTTTGTCGGT
PRIMER_LEFT_38=292,20
PRIMER_RIGHT_38=1285,22
PRIMER_LEFT_38_TM=58.378
PRIMER_RIGHT_38_TM=57.492
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=994
PRIMER_PAIR_39_PENALTY=6.275563
PRIMER_LEFT_39_PENALTY=1.622186
PRIMER_RIGHT_39_PENALTY=4.653377
PRIMER_LEFT_39_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_39_SEQUENCE=GTCTTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_39=292,20
PRIMER_RIGHT_39=1280,23
PRIMER_LEFT_39_TM=58.378
PRIMER_RIGHT_39_TM=61.653
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=47.826
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=989
PRIMER_PAIR_40_PENALTY=6.332979
PRIMER_LEFT_40_PENALTY=1.622186
PRIMER_RIGHT_40_PENALTY=4.710794
PRIMER_LEFT_40_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_40_SEQUENCE=TTAGTTTTGTCGGTGTCGGTGA
PRIMER_LEFT_40=292,20
PRIMER_RIGHT_40=1277,22
PRIMER_LEFT_40_TM=58.378
PRIMER_RIGHT_40_TM=62.711
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=986
PRIMER_PAIR_41_PENALTY=6.342591
PRIMER_LEFT_41_PENALTY=1.622186
PRIMER_RIGHT_41_PENALTY=4.720406
PRIMER_LEFT_41_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_41_SEQUENCE=CAAAGTCTTAGTTTTGTCGGTGTC
PRIMER_LEFT_41=292,20
PRIMER_RIGHT_41=1284,24
PRIMER_LEFT_41_TM=58.378
PRIMER_RIGHT_41_TM=59.280
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=41.667
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=6.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=993
PRIMER_PAIR_42_PENALTY=6.373775
PRIMER_LEFT_42_PENALTY=1.622186
PRIMER_RIGHT_42_PENALTY=4.751589
PRIMER_LEFT_42_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_42_SEQUENCE=CGGTGTCGGTGACTTTTTGTG
PRIMER_LEFT_42=292,20
PRIMER_RIGHT_42=1267,21
PRIMER_LEFT_42_TM=58.378
PRIMER_RIGHT_42_TM=63.752
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=976
PRIMER_PAIR_43_PENALTY=6.384269
PRIMER_LEFT_43_PENALTY=1.622186
PRIMER_RIGHT_43_PENALTY=4.762083
PRIMER_LEFT_43_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_43_SEQUENCE=AGTCTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_43=292,20
PRIMER_RIGHT_43=1281,23
PRIMER_LEFT_43_TM=58.378
PRIMER_RIGHT_43_TM=61.762
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=47.826
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=990
PRIMER_PAIR_44_PENALTY=6.415808
PRIMER_LEFT_44_PENALTY=1.622186
PRIMER_RIGHT_44_PENALTY=4.793622
PRIMER_LEFT_44_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_44_SEQUENCE=CCAAAGTCTTAGTTTTGTCGGTGT
PRIMER_LEFT_44=292,20
PRIMER_RIGHT_44=1285,24
PRIMER_LEFT_44_TM=58.378
PRIMER_RIGHT_44_TM=60.794
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=994
PRIMER_PAIR_45_PENALTY=6.415808
PRIMER_LEFT_45_PENALTY=1.622186
PRIMER_RIGHT_45_PENALTY=4.793622
PRIMER_LEFT_45_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_45_SEQUENCE=ACCAAAGTCTTAGTTTTGTCGGTG
PRIMER_LEFT_45=292,20
PRIMER_RIGHT_45=1286,24
PRIMER_LEFT_45_TM=58.378
PRIMER_RIGHT_45_TM=60.794
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=41.667
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=995
PRIMER_PAIR_46_PENALTY=6.540562
PRIMER_LEFT_46_PENALTY=1.622186
PRIMER_RIGHT_46_PENALTY=4.918376
PRIMER_LEFT_46_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_46_SEQUENCE=TGTCGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_46=292,20
PRIMER_RIGHT_46=1264,23
PRIMER_LEFT_46_TM=58.378
PRIMER_RIGHT_46_TM=61.918
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=43.478
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=973
PRIMER_PAIR_47_PENALTY=6.540562
PRIMER_LEFT_47_PENALTY=1.622186
PRIMER_RIGHT_47_PENALTY=4.918376
PRIMER_LEFT_47_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_47_SEQUENCE=GTGTCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_47=292,20
PRIMER_RIGHT_47=1265,23
PRIMER_LEFT_47_TM=58.378
PRIMER_RIGHT_47_TM=61.918
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=47.826
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=974
PRIMER_PAIR_48_PENALTY=6.777056
PRIMER_LEFT_48_PENALTY=1.622186
PRIMER_RIGHT_48_PENALTY=5.154871
PRIMER_LEFT_48_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_48_SEQUENCE=AGTTTTGTCGGTGTCGGTGACT
PRIMER_LEFT_48=292,20
PRIMER_RIGHT_48=1275,22
PRIMER_LEFT_48_TM=58.378
PRIMER_RIGHT_48_TM=63.155
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=984
PRIMER_PAIR_49_PENALTY=6.945041
PRIMER_LEFT_49_PENALTY=1.622186
PRIMER_RIGHT_49_PENALTY=5.322855
PRIMER_LEFT_49_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_49_SEQUENCE=CAAAGTCTTAGTTTTGTCGGTG
PRIMER_LEFT_49=292,20
PRIMER_RIGHT_49=1284,22
PRIMER_LEFT_49_TM=58.378
PRIMER_RIGHT_49_TM=56.677
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=9.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=993
=
SEQUENCE_ID=MAGIC.446_5_10066680_10066761_edi-0_zu-0_5436_20684_TRUE
SEQUENCE_TARGET=950,181
SEQUENCE_TEMPLATE=GAAANTTCNTNGGAGTTGNGTTCNATTCAGGNTANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4234061_4234094_ct-1_mt-0_15086_24832_TRUE
SEQUENCE_TARGET=950,133
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_10210649_10210818_oy-0_kn-0_360392_322342_TRUE
SEQUENCE_TARGET=950,269
SEQUENCE_TEMPLATE=CTCTTCTACTGGCTCACCGAGTCATCTTCTCATTCTCCTCANACCAAACCACTTCTTCTTTGGNTCAATGGAGGTTGNATTTTTTTTCTCCCNACTTTCCCTCGTATANTTNTANNTATACCAAAATATGTTTCGAANACTGANTCCATATCCAAATGTATCATANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATACTATTTTTGGCAAATCNAAAACAATTTNNTTNTGATAATTCTTTAANCAACTAAACNANNNNNNNNNNNNAAANNNNNNNNNNAAGAGTACCAAGTGGTTCTTAGCTTAGTGAAAGTCNGGAAGCTATCGNTCAAAAANAACCTTGTGTCCTTAATTCGATAATANTTTGGAANGATTTTCGGCACCTATGNNNNNNNNNNNNAACTCATCAATTNGCCAAAAACAATNAAAANAAAAATTANTTTCTCTATAATTGATAGTTATAGAGTAATTAAATAAAACATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAAGTTTGTNATACCAAATATATAGAATTTAAAACAATATAAGAAATCATTAGAATGTGTCACTAAGAAATNTTACATTCTTAGTATAGCTTATGTTTTTGCAATACNTAAAAATGAAAATAATTCTTATCAACACANNNNNATTCCTANATCCTCGTTAAAAANNATNTGTANCCTNGTGATTTGTGTTAGTGTANTTGTTATGTCNTCACTTNAATTCAAATNGGCGATGTACTGAAATAAAATCGTCATCAAATAAAAAATGGAAGACGTCAAATCTCATTANNNNNNNACATGATTATTCTTAAATAGACTAACTNAAAGTAAAATATCAAAAATACCTATATCAACACNTTATGTTTTATCATTATNTAAAGCAAATCAATGTCCACCAAAACAGCATCAACAATCCAATATGACTCTANTAAGTGAAACATGCAATTGTTNNNNNNNNNNNNNNNNNNNANNNACNTNNNNNTTNNTTTTTANNTTNTNTNNTNTNTNANATATNNNANNNTNNAANCNTNNNNGCANNTGNNCANNTNNNCACNTNNTCNNTNAAATAATNNNTNNGGAATNATNNTNGNAANANNCTCNTCNNNTNCAAANNNTNNNCATNNNAAGNNTGNNNANGACANGNNNANANNGANATNNNANCNNGNATANANNATNTTTTTCNTATATACTAAAANTTAAACGGACAGAAAACTAAAGCAAATGTAGCCAACCTTTATATTACTTGNTNAANCAATAGTAATAAAGAAAAAACAAGAACAATTAAATTGGANAATANTTTATTTGACTAATAAAATGAAGTTATTAGTCAAATCATAGGCTATGATCATTAAAAANAANAATTGNTGATCCAAAGCGGTTGCTTAGCCTGTTTGTCCTTTAGGATAGGCCTGGAAACATGCCTATATACTCTTGAGATTAGTTGGAAATTTTGNCAAANNTAGNATAAGTTAAGATTGGGGTAAAGAAANACTAAATTAANGAAGAACATAANTAGTTTCTTCTAATCATTGANATAANNATANAAAGAATTATTAACTAAACAAAAGCCGGCATGATGTATGAAAACTTAAAAGTAGAGTCATTTGATCAAAGATCCTTGTTACATATAATCCATTCTCATCTCAGGACCACAAAACAAAGAAAGCAANGGCATGATTGGCCAAACATNTATATAAGTATATCTCTANCCCTCTGATCACTTTCANANATCANCTTTTCAATTATTTTTCNTTCTCTTCNTGACATTTGNTAAGANTGCATGACAGAAACATACATNTATGGCCGTCTTTTGATAAACCAATNACATCAGTCGAGTNCATATCAAAATGAGACAGAAAATTGAAAACATACAATTGCTNCTTTATCCAAAATAATGTTACATGTATATAATAAAAATATTTGACCTTTTAANTTATNTTNTATNTATTAATGATAAAANNAAANTTATAAGGGATTCTATATGAAAAACAAATANCAATTTTTGTGTATATATGATCATACATGTAATTAAANTATNCAGGACCAGGCTGCTCGTCGATTGCTTATGGAGCTTCGGAGGAAATTGGACCATTTCGGATCAGCAAAACCGGTNGCAATCTNTATCTCAACAACTTTTCTTGGAACACAGGTAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.584305
PRIMER_LEFT_0_PENALTY=0.453422
PRIMER_RIGHT_0_PENALTY=0.130883
PRIMER_LEFT_0_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_0_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_0=907,20
PRIMER_RIGHT_0=1591,20
PRIMER_LEFT_0_TM=59.547
PRIMER_RIGHT_0_TM=60.131
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=685
PRIMER_PAIR_1_PENALTY=0.584305
PRIMER_LEFT_1_PENALTY=0.453422
PRIMER_RIGHT_1_PENALTY=0.130883
PRIMER_LEFT_1_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_1_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_1=906,20
PRIMER_RIGHT_1=1591,20
PRIMER_LEFT_1_TM=59.547
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=686
PRIMER_PAIR_2_PENALTY=0.682866
PRIMER_LEFT_2_PENALTY=0.551983
PRIMER_RIGHT_2_PENALTY=0.130883
PRIMER_LEFT_2_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_2_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_2=908,20
PRIMER_RIGHT_2=1591,20
PRIMER_LEFT_2_TM=60.552
PRIMER_RIGHT_2_TM=60.131
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=684
PRIMER_PAIR_3_PENALTY=0.682866
PRIMER_LEFT_3_PENALTY=0.551983
PRIMER_RIGHT_3_PENALTY=0.130883
PRIMER_LEFT_3_SEQUENCE=CAATGTCCACCAAAACAGCA
PRIMER_RIGHT_3_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_3=902,20
PRIMER_RIGHT_3=1591,20
PRIMER_LEFT_3_TM=60.552
PRIMER_RIGHT_3_TM=60.131
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=690
PRIMER_PAIR_4_PENALTY=1.155095
PRIMER_LEFT_4_PENALTY=1.024212
PRIMER_RIGHT_4_PENALTY=0.130883
PRIMER_LEFT_4_SEQUENCE=AGCAAATCAATGTCCACCAA
PRIMER_RIGHT_4_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_4=895,20
PRIMER_RIGHT_4=1591,20
PRIMER_LEFT_4_TM=58.976
PRIMER_RIGHT_4_TM=60.131
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=697
PRIMER_PAIR_5_PENALTY=1.155095
PRIMER_LEFT_5_PENALTY=1.024212
PRIMER_RIGHT_5_PENALTY=0.130883
PRIMER_LEFT_5_SEQUENCE=AAGCAAATCAATGTCCACCA
PRIMER_RIGHT_5_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_5=894,20
PRIMER_RIGHT_5=1591,20
PRIMER_LEFT_5_TM=58.976
PRIMER_RIGHT_5_TM=60.131
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=5.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=698
PRIMER_PAIR_6_PENALTY=1.259671
PRIMER_LEFT_6_PENALTY=1.128787
PRIMER_RIGHT_6_PENALTY=0.130883
PRIMER_LEFT_6_SEQUENCE=TGTCCACCAAAACAGCATCA
PRIMER_RIGHT_6_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_6=905,20
PRIMER_RIGHT_6=1591,20
PRIMER_LEFT_6_TM=61.129
PRIMER_RIGHT_6_TM=60.131
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=687
PRIMER_PAIR_7_PENALTY=1.279881
PRIMER_LEFT_7_PENALTY=1.148998
PRIMER_RIGHT_7_PENALTY=0.130883
PRIMER_LEFT_7_SEQUENCE=AATGTCCACCAAAACAGCATC
PRIMER_RIGHT_7_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_7=903,21
PRIMER_RIGHT_7=1591,20
PRIMER_LEFT_7_TM=59.851
PRIMER_RIGHT_7_TM=60.131
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=5.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=689
PRIMER_PAIR_8_PENALTY=1.489615
PRIMER_LEFT_8_PENALTY=1.358732
PRIMER_RIGHT_8_PENALTY=0.130883
PRIMER_LEFT_8_SEQUENCE=CCAAAACAGCATCAACAATCC
PRIMER_RIGHT_8_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_8=911,21
PRIMER_RIGHT_8=1591,20
PRIMER_LEFT_8_TM=60.359
PRIMER_RIGHT_8_TM=60.131
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=681
PRIMER_PAIR_9_PENALTY=1.543105
PRIMER_LEFT_9_PENALTY=1.412222
PRIMER_RIGHT_9_PENALTY=0.130883
PRIMER_LEFT_9_SEQUENCE=GTCCACCAAAACAGCATCAAC
PRIMER_RIGHT_9_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_9=906,21
PRIMER_RIGHT_9=1591,20
PRIMER_LEFT_9_TM=60.412
PRIMER_RIGHT_9_TM=60.131
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=5.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=686
PRIMER_PAIR_10_PENALTY=1.672447
PRIMER_LEFT_10_PENALTY=0.551983
PRIMER_RIGHT_10_PENALTY=1.120465
PRIMER_LEFT_10_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_10_SEQUENCE=TCATGCCGGCTTTTGTTTAG
PRIMER_LEFT_10=908,20
PRIMER_RIGHT_10=1592,20
PRIMER_LEFT_10_TM=60.552
PRIMER_RIGHT_10_TM=61.120
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=685
PRIMER_PAIR_11_PENALTY=1.687772
PRIMER_LEFT_11_PENALTY=0.453422
PRIMER_RIGHT_11_PENALTY=1.234350
PRIMER_LEFT_11_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_11_SEQUENCE=ATGCCGGCTTTTGTTTAGTT
PRIMER_LEFT_11=907,20
PRIMER_RIGHT_11=1590,20
PRIMER_LEFT_11_TM=59.547
PRIMER_RIGHT_11_TM=58.766
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=5.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=684
PRIMER_PAIR_12_PENALTY=1.687772
PRIMER_LEFT_12_PENALTY=0.453422
PRIMER_RIGHT_12_PENALTY=1.234350
PRIMER_LEFT_12_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_12_SEQUENCE=ATGCCGGCTTTTGTTTAGTT
PRIMER_LEFT_12=906,20
PRIMER_RIGHT_12=1590,20
PRIMER_LEFT_12_TM=59.547
PRIMER_RIGHT_12_TM=58.766
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=5.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=685
PRIMER_PAIR_13_PENALTY=1.715036
PRIMER_LEFT_13_PENALTY=1.584153
PRIMER_RIGHT_13_PENALTY=0.130883
PRIMER_LEFT_13_SEQUENCE=ATGTCCACCAAAACAGCATC
PRIMER_RIGHT_13_SEQUENCE=CATGCCGGCTTTTGTTTAGT
PRIMER_LEFT_13=904,20
PRIMER_RIGHT_13=1591,20
PRIMER_LEFT_13_TM=58.416
PRIMER_RIGHT_13_TM=60.131
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=5.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=688
PRIMER_PAIR_14_PENALTY=1.745192
PRIMER_LEFT_14_PENALTY=0.624727
PRIMER_RIGHT_14_PENALTY=1.120465
PRIMER_LEFT_14_SEQUENCE=GCAAATCAATGTCCACCAAA
PRIMER_RIGHT_14_SEQUENCE=TCATGCCGGCTTTTGTTTAG
PRIMER_LEFT_14=896,20
PRIMER_RIGHT_14=1592,20
PRIMER_LEFT_14_TM=59.375
PRIMER_RIGHT_14_TM=61.120
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.8000
PRIMER_RIGHT_14_END_STABILITY=6.3000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=697
PRIMER_PAIR_15_PENALTY=1.786333
PRIMER_LEFT_15_PENALTY=0.551983
PRIMER_RIGHT_15_PENALTY=1.234350
PRIMER_LEFT_15_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_15_SEQUENCE=ATGCCGGCTTTTGTTTAGTT
PRIMER_LEFT_15=908,20
PRIMER_RIGHT_15=1590,20
PRIMER_LEFT_15_TM=60.552
PRIMER_RIGHT_15_TM=58.766
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=5.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=683
PRIMER_PAIR_16_PENALTY=1.859077
PRIMER_LEFT_16_PENALTY=0.624727
PRIMER_RIGHT_16_PENALTY=1.234350
PRIMER_LEFT_16_SEQUENCE=GCAAATCAATGTCCACCAAA
PRIMER_RIGHT_16_SEQUENCE=ATGCCGGCTTTTGTTTAGTT
PRIMER_LEFT_16=896,20
PRIMER_RIGHT_16=1590,20
PRIMER_LEFT_16_TM=59.375
PRIMER_RIGHT_16_TM=58.766
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.8000
PRIMER_RIGHT_16_END_STABILITY=5.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=695
PRIMER_PAIR_17_PENALTY=1.867200
PRIMER_LEFT_17_PENALTY=0.453422
PRIMER_RIGHT_17_PENALTY=1.413779
PRIMER_LEFT_17_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_17_SEQUENCE=TGGTCCTGAGATGAGAATGG
PRIMER_LEFT_17=907,20
PRIMER_RIGHT_17=1667,20
PRIMER_LEFT_17_TM=59.547
PRIMER_RIGHT_17_TM=58.586
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=761
PRIMER_PAIR_18_PENALTY=1.867200
PRIMER_LEFT_18_PENALTY=0.453422
PRIMER_RIGHT_18_PENALTY=1.413779
PRIMER_LEFT_18_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_18_SEQUENCE=TGGTCCTGAGATGAGAATGG
PRIMER_LEFT_18=906,20
PRIMER_RIGHT_18=1667,20
PRIMER_LEFT_18_TM=59.547
PRIMER_RIGHT_18_TM=58.586
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=762
PRIMER_PAIR_19_PENALTY=1.933098
PRIMER_LEFT_19_PENALTY=0.453422
PRIMER_RIGHT_19_PENALTY=1.479676
PRIMER_LEFT_19_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_19_SEQUENCE=GTGGTCCTGAGATGAGAATGG
PRIMER_LEFT_19=907,20
PRIMER_RIGHT_19=1668,21
PRIMER_LEFT_19_TM=59.547
PRIMER_RIGHT_19_TM=59.520
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=762
PRIMER_PAIR_20_PENALTY=1.933098
PRIMER_LEFT_20_PENALTY=0.453422
PRIMER_RIGHT_20_PENALTY=1.479676
PRIMER_LEFT_20_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_20_SEQUENCE=GTGGTCCTGAGATGAGAATGG
PRIMER_LEFT_20=906,20
PRIMER_RIGHT_20=1668,21
PRIMER_LEFT_20_TM=59.547
PRIMER_RIGHT_20_TM=59.520
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=763
PRIMER_PAIR_21_PENALTY=1.965761
PRIMER_LEFT_21_PENALTY=0.551983
PRIMER_RIGHT_21_PENALTY=1.413779
PRIMER_LEFT_21_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_21_SEQUENCE=TGGTCCTGAGATGAGAATGG
PRIMER_LEFT_21=908,20
PRIMER_RIGHT_21=1667,20
PRIMER_LEFT_21_TM=60.552
PRIMER_RIGHT_21_TM=58.586
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=760
PRIMER_PAIR_22_PENALTY=1.965761
PRIMER_LEFT_22_PENALTY=0.551983
PRIMER_RIGHT_22_PENALTY=1.413779
PRIMER_LEFT_22_SEQUENCE=CAATGTCCACCAAAACAGCA
PRIMER_RIGHT_22_SEQUENCE=TGGTCCTGAGATGAGAATGG
PRIMER_LEFT_22=902,20
PRIMER_RIGHT_22=1667,20
PRIMER_LEFT_22_TM=60.552
PRIMER_RIGHT_22_TM=58.586
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=766
PRIMER_PAIR_23_PENALTY=2.031659
PRIMER_LEFT_23_PENALTY=0.551983
PRIMER_RIGHT_23_PENALTY=1.479676
PRIMER_LEFT_23_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_23_SEQUENCE=GTGGTCCTGAGATGAGAATGG
PRIMER_LEFT_23=908,20
PRIMER_RIGHT_23=1668,21
PRIMER_LEFT_23_TM=60.552
PRIMER_RIGHT_23_TM=59.520
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=761
PRIMER_PAIR_24_PENALTY=2.031659
PRIMER_LEFT_24_PENALTY=0.551983
PRIMER_RIGHT_24_PENALTY=1.479676
PRIMER_LEFT_24_SEQUENCE=CAATGTCCACCAAAACAGCA
PRIMER_RIGHT_24_SEQUENCE=GTGGTCCTGAGATGAGAATGG
PRIMER_LEFT_24=902,20
PRIMER_RIGHT_24=1668,21
PRIMER_LEFT_24_TM=60.552
PRIMER_RIGHT_24_TM=59.520
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=767
PRIMER_PAIR_25_PENALTY=2.038505
PRIMER_LEFT_25_PENALTY=0.624727
PRIMER_RIGHT_25_PENALTY=1.413779
PRIMER_LEFT_25_SEQUENCE=GCAAATCAATGTCCACCAAA
PRIMER_RIGHT_25_SEQUENCE=TGGTCCTGAGATGAGAATGG
PRIMER_LEFT_25=896,20
PRIMER_RIGHT_25=1667,20
PRIMER_LEFT_25_TM=59.375
PRIMER_RIGHT_25_TM=58.586
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.8000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=772
PRIMER_PAIR_26_PENALTY=2.059804
PRIMER_LEFT_26_PENALTY=0.453422
PRIMER_RIGHT_26_PENALTY=1.606382
PRIMER_LEFT_26_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_26_SEQUENCE=TGGTCCTGAGATGAGAATGGA
PRIMER_LEFT_26=907,20
PRIMER_RIGHT_26=1667,21
PRIMER_LEFT_26_TM=59.547
PRIMER_RIGHT_26_TM=60.606
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=761
PRIMER_PAIR_27_PENALTY=2.059804
PRIMER_LEFT_27_PENALTY=0.453422
PRIMER_RIGHT_27_PENALTY=1.606382
PRIMER_LEFT_27_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_27_SEQUENCE=TGGTCCTGAGATGAGAATGGA
PRIMER_LEFT_27=906,20
PRIMER_RIGHT_27=1667,21
PRIMER_LEFT_27_TM=59.547
PRIMER_RIGHT_27_TM=60.606
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=8.1000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=762
PRIMER_PAIR_28_PENALTY=2.104403
PRIMER_LEFT_28_PENALTY=0.624727
PRIMER_RIGHT_28_PENALTY=1.479676
PRIMER_LEFT_28_SEQUENCE=GCAAATCAATGTCCACCAAA
PRIMER_RIGHT_28_SEQUENCE=GTGGTCCTGAGATGAGAATGG
PRIMER_LEFT_28=896,20
PRIMER_RIGHT_28=1668,21
PRIMER_LEFT_28_TM=59.375
PRIMER_RIGHT_28_TM=59.520
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.8000
PRIMER_RIGHT_28_END_STABILITY=8.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=773
PRIMER_PAIR_29_PENALTY=2.144677
PRIMER_LEFT_29_PENALTY=1.024212
PRIMER_RIGHT_29_PENALTY=1.120465
PRIMER_LEFT_29_SEQUENCE=AGCAAATCAATGTCCACCAA
PRIMER_RIGHT_29_SEQUENCE=TCATGCCGGCTTTTGTTTAG
PRIMER_LEFT_29=895,20
PRIMER_RIGHT_29=1592,20
PRIMER_LEFT_29_TM=58.976
PRIMER_RIGHT_29_TM=61.120
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.3000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=698
PRIMER_PAIR_30_PENALTY=2.144677
PRIMER_LEFT_30_PENALTY=1.024212
PRIMER_RIGHT_30_PENALTY=1.120465
PRIMER_LEFT_30_SEQUENCE=AAGCAAATCAATGTCCACCA
PRIMER_RIGHT_30_SEQUENCE=TCATGCCGGCTTTTGTTTAG
PRIMER_LEFT_30=894,20
PRIMER_RIGHT_30=1592,20
PRIMER_LEFT_30_TM=58.976
PRIMER_RIGHT_30_TM=61.120
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.3000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=699
PRIMER_PAIR_31_PENALTY=2.158365
PRIMER_LEFT_31_PENALTY=0.551983
PRIMER_RIGHT_31_PENALTY=1.606382
PRIMER_LEFT_31_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_31_SEQUENCE=TGGTCCTGAGATGAGAATGGA
PRIMER_LEFT_31=908,20
PRIMER_RIGHT_31=1667,21
PRIMER_LEFT_31_TM=60.552
PRIMER_RIGHT_31_TM=60.606
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=760
PRIMER_PAIR_32_PENALTY=2.158365
PRIMER_LEFT_32_PENALTY=0.551983
PRIMER_RIGHT_32_PENALTY=1.606382
PRIMER_LEFT_32_SEQUENCE=CAATGTCCACCAAAACAGCA
PRIMER_RIGHT_32_SEQUENCE=TGGTCCTGAGATGAGAATGGA
PRIMER_LEFT_32=902,20
PRIMER_RIGHT_32=1667,21
PRIMER_LEFT_32_TM=60.552
PRIMER_RIGHT_32_TM=60.606
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=766
PRIMER_PAIR_33_PENALTY=2.190881
PRIMER_LEFT_33_PENALTY=0.453422
PRIMER_RIGHT_33_PENALTY=1.737459
PRIMER_LEFT_33_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_33_SEQUENCE=GCATGTTTCCAGGCCTATCC
PRIMER_LEFT_33=907,20
PRIMER_RIGHT_33=1436,20
PRIMER_LEFT_33_TM=59.547
PRIMER_RIGHT_33_TM=61.737
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.1000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=530
PRIMER_PAIR_34_PENALTY=2.190881
PRIMER_LEFT_34_PENALTY=0.453422
PRIMER_RIGHT_34_PENALTY=1.737459
PRIMER_LEFT_34_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_34_SEQUENCE=GGCATGTTTCCAGGCCTATC
PRIMER_LEFT_34=907,20
PRIMER_RIGHT_34=1437,20
PRIMER_LEFT_34_TM=59.547
PRIMER_RIGHT_34_TM=61.737
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=5.6000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=531
PRIMER_PAIR_35_PENALTY=2.190881
PRIMER_LEFT_35_PENALTY=0.453422
PRIMER_RIGHT_35_PENALTY=1.737459
PRIMER_LEFT_35_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_35_SEQUENCE=GCATGTTTCCAGGCCTATCC
PRIMER_LEFT_35=906,20
PRIMER_RIGHT_35=1436,20
PRIMER_LEFT_35_TM=59.547
PRIMER_RIGHT_35_TM=61.737
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=7.1000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=531
PRIMER_PAIR_36_PENALTY=2.190881
PRIMER_LEFT_36_PENALTY=0.453422
PRIMER_RIGHT_36_PENALTY=1.737459
PRIMER_LEFT_36_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_36_SEQUENCE=GGCATGTTTCCAGGCCTATC
PRIMER_LEFT_36=906,20
PRIMER_RIGHT_36=1437,20
PRIMER_LEFT_36_TM=59.547
PRIMER_RIGHT_36_TM=61.737
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=5.6000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=532
PRIMER_PAIR_37_PENALTY=2.231109
PRIMER_LEFT_37_PENALTY=0.624727
PRIMER_RIGHT_37_PENALTY=1.606382
PRIMER_LEFT_37_SEQUENCE=GCAAATCAATGTCCACCAAA
PRIMER_RIGHT_37_SEQUENCE=TGGTCCTGAGATGAGAATGGA
PRIMER_LEFT_37=896,20
PRIMER_RIGHT_37=1667,21
PRIMER_LEFT_37_TM=59.375
PRIMER_RIGHT_37_TM=60.606
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.8000
PRIMER_RIGHT_37_END_STABILITY=8.1000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=772
PRIMER_PAIR_38_PENALTY=2.249252
PRIMER_LEFT_38_PENALTY=1.128787
PRIMER_RIGHT_38_PENALTY=1.120465
PRIMER_LEFT_38_SEQUENCE=TGTCCACCAAAACAGCATCA
PRIMER_RIGHT_38_SEQUENCE=TCATGCCGGCTTTTGTTTAG
PRIMER_LEFT_38=905,20
PRIMER_RIGHT_38=1592,20
PRIMER_LEFT_38_TM=61.129
PRIMER_RIGHT_38_TM=61.120
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=688
PRIMER_PAIR_39_PENALTY=2.258562
PRIMER_LEFT_39_PENALTY=1.024212
PRIMER_RIGHT_39_PENALTY=1.234350
PRIMER_LEFT_39_SEQUENCE=AGCAAATCAATGTCCACCAA
PRIMER_RIGHT_39_SEQUENCE=ATGCCGGCTTTTGTTTAGTT
PRIMER_LEFT_39=895,20
PRIMER_RIGHT_39=1590,20
PRIMER_LEFT_39_TM=58.976
PRIMER_RIGHT_39_TM=58.766
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=5.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=696
PRIMER_PAIR_40_PENALTY=2.258562
PRIMER_LEFT_40_PENALTY=1.024212
PRIMER_RIGHT_40_PENALTY=1.234350
PRIMER_LEFT_40_SEQUENCE=AAGCAAATCAATGTCCACCA
PRIMER_RIGHT_40_SEQUENCE=ATGCCGGCTTTTGTTTAGTT
PRIMER_LEFT_40=894,20
PRIMER_RIGHT_40=1590,20
PRIMER_LEFT_40_TM=58.976
PRIMER_RIGHT_40_TM=58.766
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=5.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=697
PRIMER_PAIR_41_PENALTY=2.289442
PRIMER_LEFT_41_PENALTY=0.551983
PRIMER_RIGHT_41_PENALTY=1.737459
PRIMER_LEFT_41_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_41_SEQUENCE=GCATGTTTCCAGGCCTATCC
PRIMER_LEFT_41=908,20
PRIMER_RIGHT_41=1436,20
PRIMER_LEFT_41_TM=60.552
PRIMER_RIGHT_41_TM=61.737
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=529
PRIMER_PAIR_42_PENALTY=2.289442
PRIMER_LEFT_42_PENALTY=0.551983
PRIMER_RIGHT_42_PENALTY=1.737459
PRIMER_LEFT_42_SEQUENCE=CCACCAAAACAGCATCAACA
PRIMER_RIGHT_42_SEQUENCE=GGCATGTTTCCAGGCCTATC
PRIMER_LEFT_42=908,20
PRIMER_RIGHT_42=1437,20
PRIMER_LEFT_42_TM=60.552
PRIMER_RIGHT_42_TM=61.737
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=5.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=530
PRIMER_PAIR_43_PENALTY=2.289442
PRIMER_LEFT_43_PENALTY=0.551983
PRIMER_RIGHT_43_PENALTY=1.737459
PRIMER_LEFT_43_SEQUENCE=CAATGTCCACCAAAACAGCA
PRIMER_RIGHT_43_SEQUENCE=GCATGTTTCCAGGCCTATCC
PRIMER_LEFT_43=902,20
PRIMER_RIGHT_43=1436,20
PRIMER_LEFT_43_TM=60.552
PRIMER_RIGHT_43_TM=61.737
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=7.1000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=535
PRIMER_PAIR_44_PENALTY=2.289442
PRIMER_LEFT_44_PENALTY=0.551983
PRIMER_RIGHT_44_PENALTY=1.737459
PRIMER_LEFT_44_SEQUENCE=CAATGTCCACCAAAACAGCA
PRIMER_RIGHT_44_SEQUENCE=GGCATGTTTCCAGGCCTATC
PRIMER_LEFT_44=902,20
PRIMER_RIGHT_44=1437,20
PRIMER_LEFT_44_TM=60.552
PRIMER_RIGHT_44_TM=61.737
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=5.6000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=536
PRIMER_PAIR_45_PENALTY=2.329695
PRIMER_LEFT_45_PENALTY=0.453422
PRIMER_RIGHT_45_PENALTY=1.876274
PRIMER_LEFT_45_SEQUENCE=TCCACCAAAACAGCATCAAC
PRIMER_RIGHT_45_SEQUENCE=TGTTTTGTGGTCCTGAGATGA
PRIMER_LEFT_45=907,20
PRIMER_RIGHT_45=1674,21
PRIMER_LEFT_45_TM=59.547
PRIMER_RIGHT_45_TM=59.124
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.6000
PRIMER_PAIR_45_COMPL_ANY=8.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=768
PRIMER_PAIR_46_PENALTY=2.329695
PRIMER_LEFT_46_PENALTY=0.453422
PRIMER_RIGHT_46_PENALTY=1.876274
PRIMER_LEFT_46_SEQUENCE=GTCCACCAAAACAGCATCAA
PRIMER_RIGHT_46_SEQUENCE=TGTTTTGTGGTCCTGAGATGA
PRIMER_LEFT_46=906,20
PRIMER_RIGHT_46=1674,21
PRIMER_LEFT_46_TM=59.547
PRIMER_RIGHT_46_TM=59.124
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=6.6000
PRIMER_PAIR_46_COMPL_ANY=8.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=769
PRIMER_PAIR_47_PENALTY=2.362186
PRIMER_LEFT_47_PENALTY=0.624727
PRIMER_RIGHT_47_PENALTY=1.737459
PRIMER_LEFT_47_SEQUENCE=GCAAATCAATGTCCACCAAA
PRIMER_RIGHT_47_SEQUENCE=GCATGTTTCCAGGCCTATCC
PRIMER_LEFT_47=896,20
PRIMER_RIGHT_47=1436,20
PRIMER_LEFT_47_TM=59.375
PRIMER_RIGHT_47_TM=61.737
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.8000
PRIMER_RIGHT_47_END_STABILITY=7.1000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=541
PRIMER_PAIR_48_PENALTY=2.362186
PRIMER_LEFT_48_PENALTY=0.624727
PRIMER_RIGHT_48_PENALTY=1.737459
PRIMER_LEFT_48_SEQUENCE=GCAAATCAATGTCCACCAAA
PRIMER_RIGHT_48_SEQUENCE=GGCATGTTTCCAGGCCTATC
PRIMER_LEFT_48=896,20
PRIMER_RIGHT_48=1437,20
PRIMER_LEFT_48_TM=59.375
PRIMER_RIGHT_48_TM=61.737
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.8000
PRIMER_RIGHT_48_END_STABILITY=5.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=542
PRIMER_PAIR_49_PENALTY=2.363138
PRIMER_LEFT_49_PENALTY=1.128787
PRIMER_RIGHT_49_PENALTY=1.234350
PRIMER_LEFT_49_SEQUENCE=TGTCCACCAAAACAGCATCA
PRIMER_RIGHT_49_SEQUENCE=ATGCCGGCTTTTGTTTAGTT
PRIMER_LEFT_49=905,20
PRIMER_RIGHT_49=1590,20
PRIMER_LEFT_49_TM=61.129
PRIMER_RIGHT_49_TM=58.766
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=5.7000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=686
=
SEQUENCE_ID=MAGIC.149_3_12777980_12778032_no-0_oy-0_6247_6779_TRUE
SEQUENCE_TARGET=950,152
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_9011420_9011486_ler-0_hi-0_1651701_5472_TRUE
SEQUENCE_TARGET=950,166
SEQUENCE_TEMPLATE=GNTAATAATCATATTGNTAGCNTCAAATCAGGAAAAGACATCTCATTATGGATNAAACTATATAAGCAATCTAAATATTACACGGACTTATNTCTATTGTAAGCANNNNNNNNNNNCCCCCATGTGCATGTAAACTTATTGATGCTTATCAAGGTTGTCNTANAGAAAATCGAATTCATGAAATAAAGAACTTTTGAATNAAAAATGCGATGGTTACAGTTACATAATTTACAAGCACCCTGAAAAGACAATGTAACTGAAATTTTGATTTGAGAGAAATCAGTNTATCNNAAAAANAAAANAGAGAAANCAATCTATATATGTACTTTATTANTAGAAAAATATAGNATTACATTANAAAGTATATNATANTATATGGAAAAGGTGGGANGTTTAAGGAGGGCATTACTCGAAGCACATTTGTTTTGGCNTTGACTTTGGCATGTAAGACAAGCCACATGGCCGGCCGGTTATCACCGTTAATCAAGACACATGANCATTGCTTNGATTTATTTTNTTTGNTAATACAATTNGNTTTACTTTATAATCCACCGTANTNNNNNNNNNNNAANNATTTTTNGNTGGTTCTTGACTCTATTAGTTTATATTTTTCAGATGAATATTTAATAAGNNATNCACANTTTTTTNNTTTCTGTTTTTANANTNTCGACACGAAAAAATTGCGTCACTCGTCAATCAAAAGNTTGATNTGGAGTTTTATATTTTAAACTATTATATANGTTAAANNAANAANAGNNNTACATTNNTCTTCCNANNNCNTAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_13920132_13920427_oy-0_ct-1_361201_51265_TRUE
SEQUENCE_TARGET=950,395
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_13284628_13284641_ler-0_oy-0_67279_172242_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATAGNTAATGAGTAACCCGTTTTATTTATAAAACACACGCCATTTATATATGTTTTTGTTNNTGATGAGCCACATAACTTATAGATTTNAATTACCGATGANAACATAAACATGNGTAGATAATGAGTAACNTGCTTTATTTATTAAACANACGATATATATAGATGTATTTGTTNATTTTTTTTTTAATTCTATAAACNANATACNTTTTAGATTTCANANATGCANTTGATATTNAANCNCTAANTTATATCACTAGATATGCCAAAAACNGANCATATATATATACANATNTTTGCTTTGTATGCGACTANTTATTCAATNGANACTAACANANAAAAANGTCATTGGTAACTGTAACATAGAATGTTATTTAAAAACAACACATATAATTNNTATATGCAGACGNTGTNTGAAAACAATGGGCGTGAGTTGGAAATTGTGTATTGGNCNATAGACTGGTCAAAGTATCAACTTATAGAGATCATACATTACTTGCAATGTTTCGTTATATCAGTAATCATTAGTATGCAACTTGCTACATAATATGGTCNCCAAATCTTATTTTGCGATTTGACAATGTAAATCTATCCGTAGGAAAATNTTTGATANGTATCTATTNCATNAAATGNGGCGACNGTGTTATATACGGGTTCCAATATTGCATTGGGGGAAGGGGTGAAATAAATTCCAAAAACAACGTGTTTCGTATAAATATAACATAGGATAACCACTAAGCATCCACNAGATAAACGACACGTNGCGCATGACGTCTGCAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNGTAGCGANANNTTCCATATATNACAGGAGAGATGANTAGCATTTAAAACACANGATAATGAGTAACCCGCTTTATTTATAAAACACACGCCATCTATATATGGCGTTGTTCTGGATGAGCCACATAACTTATAGATTTCAATTACTAATGAGAACGTAAACATGCGTAGGTAATGAGTAATCCGCTTTATTTATAAAACACACNNNNNNNNTATANATATATATATATGTATTTGTTNCTTTTTTTTAATTCTATAAACNNCATACNTTATAGATTTCAATTACANATGCATTTGATATTTAAACCCTNNCTTATATCACTAGATATGCNAAAAACGNACCATATATATACANATGTTTACTTTGTATGCNACTAGTTATTCAATGAAGACTAATAGATAAAATGGTCATTGGTAACTGTAAAATAGAACGTTATTTAAAAACAACTCATGTAATTCCTATATGCAAACNCGGTATGAAAAGAATAGGCGTGAGTTGNAAATTGTGTATTGGGTTATAGCCTCGGCGAAGTNTCANNNNNNNNNNNNNNNACATNACATNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.932643
PRIMER_LEFT_0_PENALTY=0.022341
PRIMER_RIGHT_0_PENALTY=0.910302
PRIMER_LEFT_0_SEQUENCE=GAAAACAATGGGCGTGAGTT
PRIMER_RIGHT_0_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_0=493,20
PRIMER_RIGHT_0=1195,20
PRIMER_LEFT_0_TM=59.978
PRIMER_RIGHT_0_TM=59.090
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=703
PRIMER_PAIR_1_PENALTY=1.784044
PRIMER_LEFT_1_PENALTY=0.873742
PRIMER_RIGHT_1_PENALTY=0.910302
PRIMER_LEFT_1_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_1_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_1=736,20
PRIMER_RIGHT_1=1195,20
PRIMER_LEFT_1_TM=60.874
PRIMER_RIGHT_1_TM=59.090
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=460
PRIMER_PAIR_2_PENALTY=1.834052
PRIMER_LEFT_2_PENALTY=0.923750
PRIMER_RIGHT_2_PENALTY=0.910302
PRIMER_LEFT_2_SEQUENCE=AAAACAATGGGCGTGAGTTG
PRIMER_RIGHT_2_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_2=494,20
PRIMER_RIGHT_2=1195,20
PRIMER_LEFT_2_TM=60.924
PRIMER_RIGHT_2_TM=59.090
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=702
PRIMER_PAIR_3_PENALTY=1.884734
PRIMER_LEFT_3_PENALTY=0.022341
PRIMER_RIGHT_3_PENALTY=1.862393
PRIMER_LEFT_3_SEQUENCE=GAAAACAATGGGCGTGAGTT
PRIMER_RIGHT_3_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_3=493,20
PRIMER_RIGHT_3=1196,21
PRIMER_LEFT_3_TM=59.978
PRIMER_RIGHT_3_TM=59.138
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=704
PRIMER_PAIR_4_PENALTY=2.285670
PRIMER_LEFT_4_PENALTY=1.375368
PRIMER_RIGHT_4_PENALTY=0.910302
PRIMER_LEFT_4_SEQUENCE=GCGTGAGTTGGAAATTGTGT
PRIMER_RIGHT_4_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_4=504,20
PRIMER_RIGHT_4=1195,20
PRIMER_LEFT_4_TM=58.625
PRIMER_RIGHT_4_TM=59.090
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=692
PRIMER_PAIR_5_PENALTY=2.384807
PRIMER_LEFT_5_PENALTY=1.474506
PRIMER_RIGHT_5_PENALTY=0.910302
PRIMER_LEFT_5_SEQUENCE=GGCGTGAGTTGGAAATTGTG
PRIMER_RIGHT_5_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_5=503,20
PRIMER_RIGHT_5=1195,20
PRIMER_LEFT_5_TM=61.475
PRIMER_RIGHT_5_TM=59.090
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=693
PRIMER_PAIR_6_PENALTY=2.384807
PRIMER_LEFT_6_PENALTY=1.474506
PRIMER_RIGHT_6_PENALTY=0.910302
PRIMER_LEFT_6_SEQUENCE=TGAAAACAATGGGCGTGAGT
PRIMER_RIGHT_6_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_6=492,20
PRIMER_RIGHT_6=1195,20
PRIMER_LEFT_6_TM=61.475
PRIMER_RIGHT_6_TM=59.090
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=704
PRIMER_PAIR_7_PENALTY=2.736135
PRIMER_LEFT_7_PENALTY=0.873742
PRIMER_RIGHT_7_PENALTY=1.862393
PRIMER_LEFT_7_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_7_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_7=736,20
PRIMER_RIGHT_7=1196,21
PRIMER_LEFT_7_TM=60.874
PRIMER_RIGHT_7_TM=59.138
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=461
PRIMER_PAIR_8_PENALTY=2.786143
PRIMER_LEFT_8_PENALTY=0.923750
PRIMER_RIGHT_8_PENALTY=1.862393
PRIMER_LEFT_8_SEQUENCE=AAAACAATGGGCGTGAGTTG
PRIMER_RIGHT_8_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_8=494,20
PRIMER_RIGHT_8=1196,21
PRIMER_LEFT_8_TM=60.924
PRIMER_RIGHT_8_TM=59.138
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=703
PRIMER_PAIR_9_PENALTY=2.974232
PRIMER_LEFT_9_PENALTY=2.063930
PRIMER_RIGHT_9_PENALTY=0.910302
PRIMER_LEFT_9_SEQUENCE=GGGGGAAGGGGTGAAATAAA
PRIMER_RIGHT_9_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_9=747,20
PRIMER_RIGHT_9=1195,20
PRIMER_LEFT_9_TM=62.064
PRIMER_RIGHT_9_TM=59.090
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.2000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=449
PRIMER_PAIR_10_PENALTY=3.222000
PRIMER_LEFT_10_PENALTY=2.063930
PRIMER_RIGHT_10_PENALTY=1.158069
PRIMER_LEFT_10_SEQUENCE=GGGGGAAGGGGTGAAATAAA
PRIMER_RIGHT_10_SEQUENCE=GCCGAGGCTATAACCCAATAC
PRIMER_LEFT_10=747,20
PRIMER_RIGHT_10=1596,21
PRIMER_LEFT_10_TM=62.064
PRIMER_RIGHT_10_TM=59.842
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.2000
PRIMER_RIGHT_10_END_STABILITY=5.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=850
PRIMER_PAIR_11_PENALTY=3.237761
PRIMER_LEFT_11_PENALTY=1.375368
PRIMER_RIGHT_11_PENALTY=1.862393
PRIMER_LEFT_11_SEQUENCE=GCGTGAGTTGGAAATTGTGT
PRIMER_RIGHT_11_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_11=504,20
PRIMER_RIGHT_11=1196,21
PRIMER_LEFT_11_TM=58.625
PRIMER_RIGHT_11_TM=59.138
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=693
PRIMER_PAIR_12_PENALTY=3.336898
PRIMER_LEFT_12_PENALTY=1.474506
PRIMER_RIGHT_12_PENALTY=1.862393
PRIMER_LEFT_12_SEQUENCE=GGCGTGAGTTGGAAATTGTG
PRIMER_RIGHT_12_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_12=503,20
PRIMER_RIGHT_12=1196,21
PRIMER_LEFT_12_TM=61.475
PRIMER_RIGHT_12_TM=59.138
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=694
PRIMER_PAIR_13_PENALTY=3.336898
PRIMER_LEFT_13_PENALTY=1.474506
PRIMER_RIGHT_13_PENALTY=1.862393
PRIMER_LEFT_13_SEQUENCE=TGAAAACAATGGGCGTGAGT
PRIMER_RIGHT_13_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_13=492,20
PRIMER_RIGHT_13=1196,21
PRIMER_LEFT_13_TM=61.475
PRIMER_RIGHT_13_TM=59.138
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=705
PRIMER_PAIR_14_PENALTY=3.471851
PRIMER_LEFT_14_PENALTY=0.873742
PRIMER_RIGHT_14_PENALTY=2.598109
PRIMER_LEFT_14_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_14_SEQUENCE=CAACTCACGCCTATTCTTTTCA
PRIMER_LEFT_14=736,20
PRIMER_RIGHT_14=1567,22
PRIMER_LEFT_14_TM=60.874
PRIMER_RIGHT_14_TM=59.402
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=832
PRIMER_PAIR_15_PENALTY=3.555248
PRIMER_LEFT_15_PENALTY=0.022341
PRIMER_RIGHT_15_PENALTY=3.532907
PRIMER_LEFT_15_SEQUENCE=GAAAACAATGGGCGTGAGTT
PRIMER_RIGHT_15_SEQUENCE=CCTACGCATGTTTACGTTCTC
PRIMER_LEFT_15=493,20
PRIMER_RIGHT_15=1240,21
PRIMER_LEFT_15_TM=59.978
PRIMER_RIGHT_15_TM=57.467
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=748
PRIMER_PAIR_16_PENALTY=3.873297
PRIMER_LEFT_16_PENALTY=2.962996
PRIMER_RIGHT_16_PENALTY=0.910302
PRIMER_LEFT_16_SEQUENCE=ATATTGCATTGGGGGAAGGG
PRIMER_RIGHT_16_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_16=737,20
PRIMER_RIGHT_16=1195,20
PRIMER_LEFT_16_TM=62.963
PRIMER_RIGHT_16_TM=59.090
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=459
PRIMER_PAIR_17_PENALTY=3.912468
PRIMER_LEFT_17_PENALTY=0.022341
PRIMER_RIGHT_17_PENALTY=3.890127
PRIMER_LEFT_17_SEQUENCE=GAAAACAATGGGCGTGAGTT
PRIMER_RIGHT_17_SEQUENCE=TTATGTGGCTCATCCAGAAC
PRIMER_LEFT_17=493,20
PRIMER_RIGHT_17=1197,20
PRIMER_LEFT_17_TM=59.978
PRIMER_RIGHT_17_TM=56.110
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=705
PRIMER_PAIR_18_PENALTY=3.926323
PRIMER_LEFT_18_PENALTY=2.063930
PRIMER_RIGHT_18_PENALTY=1.862393
PRIMER_LEFT_18_SEQUENCE=GGGGGAAGGGGTGAAATAAA
PRIMER_RIGHT_18_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_18=747,20
PRIMER_RIGHT_18=1196,21
PRIMER_LEFT_18_TM=62.064
PRIMER_RIGHT_18_TM=59.138
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=450
PRIMER_PAIR_19_PENALTY=4.055801
PRIMER_LEFT_19_PENALTY=3.145499
PRIMER_RIGHT_19_PENALTY=0.910302
PRIMER_LEFT_19_SEQUENCE=AAACAATGGGCGTGAGTTGG
PRIMER_RIGHT_19_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_19=495,20
PRIMER_RIGHT_19=1195,20
PRIMER_LEFT_19_TM=63.145
PRIMER_RIGHT_19_TM=59.090
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=701
PRIMER_PAIR_20_PENALTY=4.087519
PRIMER_LEFT_20_PENALTY=2.063930
PRIMER_RIGHT_20_PENALTY=2.023588
PRIMER_LEFT_20_SEQUENCE=GGGGGAAGGGGTGAAATAAA
PRIMER_RIGHT_20_SEQUENCE=CCGAGGCTATAACCCAATACA
PRIMER_LEFT_20=747,20
PRIMER_RIGHT_20=1595,21
PRIMER_LEFT_20_TM=62.064
PRIMER_RIGHT_20_TM=58.976
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.2000
PRIMER_RIGHT_20_END_STABILITY=5.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=849
PRIMER_PAIR_21_PENALTY=4.121065
PRIMER_LEFT_21_PENALTY=2.962996
PRIMER_RIGHT_21_PENALTY=1.158069
PRIMER_LEFT_21_SEQUENCE=ATATTGCATTGGGGGAAGGG
PRIMER_RIGHT_21_SEQUENCE=GCCGAGGCTATAACCCAATAC
PRIMER_LEFT_21=737,20
PRIMER_RIGHT_21=1596,21
PRIMER_LEFT_21_TM=62.963
PRIMER_RIGHT_21_TM=59.842
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=5.6000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=860
PRIMER_PAIR_22_PENALTY=4.146084
PRIMER_LEFT_22_PENALTY=3.235782
PRIMER_RIGHT_22_PENALTY=0.910302
PRIMER_LEFT_22_SEQUENCE=GGCGTGAGTTGGAAATTGTGT
PRIMER_RIGHT_22_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_22=503,21
PRIMER_RIGHT_22=1195,20
PRIMER_LEFT_22_TM=62.236
PRIMER_RIGHT_22_TM=59.090
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=693
PRIMER_PAIR_23_PENALTY=4.306288
PRIMER_LEFT_23_PENALTY=2.063930
PRIMER_RIGHT_23_PENALTY=2.242357
PRIMER_LEFT_23_SEQUENCE=GGGGGAAGGGGTGAAATAAA
PRIMER_RIGHT_23_SEQUENCE=CCGAGGCTATAACCCAATACAC
PRIMER_LEFT_23=747,20
PRIMER_RIGHT_23=1595,22
PRIMER_LEFT_23_TM=62.064
PRIMER_RIGHT_23_TM=59.758
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.2000
PRIMER_RIGHT_23_END_STABILITY=5.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=849
PRIMER_PAIR_24_PENALTY=4.406649
PRIMER_LEFT_24_PENALTY=0.873742
PRIMER_RIGHT_24_PENALTY=3.532907
PRIMER_LEFT_24_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_24_SEQUENCE=CCTACGCATGTTTACGTTCTC
PRIMER_LEFT_24=736,20
PRIMER_RIGHT_24=1240,21
PRIMER_LEFT_24_TM=60.874
PRIMER_RIGHT_24_TM=57.467
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=505
PRIMER_PAIR_25_PENALTY=4.407649
PRIMER_LEFT_25_PENALTY=3.497347
PRIMER_RIGHT_25_PENALTY=0.910302
PRIMER_LEFT_25_SEQUENCE=TGGGGGAAGGGGTGAAATAA
PRIMER_RIGHT_25_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_25=746,20
PRIMER_RIGHT_25=1195,20
PRIMER_LEFT_25_TM=63.497
PRIMER_RIGHT_25_TM=59.090
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.2000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=450
PRIMER_PAIR_26_PENALTY=4.456657
PRIMER_LEFT_26_PENALTY=0.923750
PRIMER_RIGHT_26_PENALTY=3.532907
PRIMER_LEFT_26_SEQUENCE=AAAACAATGGGCGTGAGTTG
PRIMER_RIGHT_26_SEQUENCE=CCTACGCATGTTTACGTTCTC
PRIMER_LEFT_26=494,20
PRIMER_RIGHT_26=1240,21
PRIMER_LEFT_26_TM=60.924
PRIMER_RIGHT_26_TM=57.467
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=747
PRIMER_PAIR_27_PENALTY=4.495089
PRIMER_LEFT_27_PENALTY=0.022341
PRIMER_RIGHT_27_PENALTY=4.472748
PRIMER_LEFT_27_SEQUENCE=GAAAACAATGGGCGTGAGTT
PRIMER_RIGHT_27_SEQUENCE=CCTACGCATGTTTACGTTCT
PRIMER_LEFT_27=493,20
PRIMER_RIGHT_27=1240,20
PRIMER_LEFT_27_TM=59.978
PRIMER_RIGHT_27_TM=55.527
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=748
PRIMER_PAIR_28_PENALTY=4.534772
PRIMER_LEFT_28_PENALTY=0.873742
PRIMER_RIGHT_28_PENALTY=3.661030
PRIMER_LEFT_28_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_28_SEQUENCE=ACCTACGCATGTTTACGTTCTC
PRIMER_LEFT_28=736,20
PRIMER_RIGHT_28=1241,22
PRIMER_LEFT_28_TM=60.874
PRIMER_RIGHT_28_TM=58.339
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=506
PRIMER_PAIR_29_PENALTY=4.584780
PRIMER_LEFT_29_PENALTY=0.923750
PRIMER_RIGHT_29_PENALTY=3.661030
PRIMER_LEFT_29_SEQUENCE=AAAACAATGGGCGTGAGTTG
PRIMER_RIGHT_29_SEQUENCE=ACCTACGCATGTTTACGTTCTC
PRIMER_LEFT_29=494,20
PRIMER_RIGHT_29=1241,22
PRIMER_LEFT_29_TM=60.924
PRIMER_RIGHT_29_TM=58.339
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=748
PRIMER_PAIR_30_PENALTY=4.599256
PRIMER_LEFT_30_PENALTY=0.873742
PRIMER_RIGHT_30_PENALTY=3.725514
PRIMER_LEFT_30_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_30_SEQUENCE=TCACGCCTATTCTTTTCATACC
PRIMER_LEFT_30=736,20
PRIMER_RIGHT_30=1563,22
PRIMER_LEFT_30_TM=60.874
PRIMER_RIGHT_30_TM=58.274
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.8000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=828
PRIMER_PAIR_31_PENALTY=4.608929
PRIMER_LEFT_31_PENALTY=3.698627
PRIMER_RIGHT_31_PENALTY=0.910302
PRIMER_LEFT_31_SEQUENCE=GAAAACAATGGGCGTGAGTTG
PRIMER_RIGHT_31_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_31=493,21
PRIMER_RIGHT_31=1195,20
PRIMER_LEFT_31_TM=62.699
PRIMER_RIGHT_31_TM=59.090
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=703
PRIMER_PAIR_32_PENALTY=4.608929
PRIMER_LEFT_32_PENALTY=3.698627
PRIMER_RIGHT_32_PENALTY=0.910302
PRIMER_LEFT_32_SEQUENCE=TGAAAACAATGGGCGTGAGTT
PRIMER_RIGHT_32_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_32=492,21
PRIMER_RIGHT_32=1195,20
PRIMER_LEFT_32_TM=62.699
PRIMER_RIGHT_32_TM=59.090
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=704
PRIMER_PAIR_33_PENALTY=4.655417
PRIMER_LEFT_33_PENALTY=3.497347
PRIMER_RIGHT_33_PENALTY=1.158069
PRIMER_LEFT_33_SEQUENCE=TGGGGGAAGGGGTGAAATAA
PRIMER_RIGHT_33_SEQUENCE=GCCGAGGCTATAACCCAATAC
PRIMER_LEFT_33=746,20
PRIMER_RIGHT_33=1596,21
PRIMER_LEFT_33_TM=63.497
PRIMER_RIGHT_33_TM=59.842
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.2000
PRIMER_RIGHT_33_END_STABILITY=5.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=851
PRIMER_PAIR_34_PENALTY=4.657662
PRIMER_LEFT_34_PENALTY=3.747360
PRIMER_RIGHT_34_PENALTY=0.910302
PRIMER_LEFT_34_SEQUENCE=AACAATGGGCGTGAGTTGGA
PRIMER_RIGHT_34_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_34=496,20
PRIMER_RIGHT_34=1195,20
PRIMER_LEFT_34_TM=63.747
PRIMER_RIGHT_34_TM=59.090
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=700
PRIMER_PAIR_35_PENALTY=4.676759
PRIMER_LEFT_35_PENALTY=0.873742
PRIMER_RIGHT_35_PENALTY=3.803017
PRIMER_LEFT_35_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_35_SEQUENCE=CTCACGCCTATTCTTTTCATACC
PRIMER_LEFT_35=736,20
PRIMER_RIGHT_35=1564,23
PRIMER_LEFT_35_TM=60.874
PRIMER_RIGHT_35_TM=59.197
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=43.478
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.8000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=829
PRIMER_PAIR_36_PENALTY=4.763869
PRIMER_LEFT_36_PENALTY=0.873742
PRIMER_RIGHT_36_PENALTY=3.890127
PRIMER_LEFT_36_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_36_SEQUENCE=TTATGTGGCTCATCCAGAAC
PRIMER_LEFT_36=736,20
PRIMER_RIGHT_36=1197,20
PRIMER_LEFT_36_TM=60.874
PRIMER_RIGHT_36_TM=56.110
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=462
PRIMER_PAIR_37_PENALTY=4.813877
PRIMER_LEFT_37_PENALTY=0.923750
PRIMER_RIGHT_37_PENALTY=3.890127
PRIMER_LEFT_37_SEQUENCE=AAAACAATGGGCGTGAGTTG
PRIMER_RIGHT_37_SEQUENCE=TTATGTGGCTCATCCAGAAC
PRIMER_LEFT_37=494,20
PRIMER_RIGHT_37=1197,20
PRIMER_LEFT_37_TM=60.924
PRIMER_RIGHT_37_TM=56.110
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=704
PRIMER_PAIR_38_PENALTY=4.825388
PRIMER_LEFT_38_PENALTY=2.962996
PRIMER_RIGHT_38_PENALTY=1.862393
PRIMER_LEFT_38_SEQUENCE=ATATTGCATTGGGGGAAGGG
PRIMER_RIGHT_38_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_38=737,20
PRIMER_RIGHT_38=1196,21
PRIMER_LEFT_38_TM=62.963
PRIMER_RIGHT_38_TM=59.138
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=460
PRIMER_PAIR_39_PENALTY=4.908275
PRIMER_LEFT_39_PENALTY=1.375368
PRIMER_RIGHT_39_PENALTY=3.532907
PRIMER_LEFT_39_SEQUENCE=GCGTGAGTTGGAAATTGTGT
PRIMER_RIGHT_39_SEQUENCE=CCTACGCATGTTTACGTTCTC
PRIMER_LEFT_39=504,20
PRIMER_RIGHT_39=1240,21
PRIMER_LEFT_39_TM=58.625
PRIMER_RIGHT_39_TM=57.467
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=737
PRIMER_PAIR_40_PENALTY=4.942117
PRIMER_LEFT_40_PENALTY=0.873742
PRIMER_RIGHT_40_PENALTY=4.068375
PRIMER_LEFT_40_SEQUENCE=AATATTGCATTGGGGGAAGG
PRIMER_RIGHT_40_SEQUENCE=ACTCACGCCTATTCTTTTCATACC
PRIMER_LEFT_40=736,20
PRIMER_RIGHT_40=1565,24
PRIMER_LEFT_40_TM=60.874
PRIMER_RIGHT_40_TM=59.932
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=41.667
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.8000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=830
PRIMER_PAIR_41_PENALTY=5.007412
PRIMER_LEFT_41_PENALTY=1.474506
PRIMER_RIGHT_41_PENALTY=3.532907
PRIMER_LEFT_41_SEQUENCE=GGCGTGAGTTGGAAATTGTG
PRIMER_RIGHT_41_SEQUENCE=CCTACGCATGTTTACGTTCTC
PRIMER_LEFT_41=503,20
PRIMER_RIGHT_41=1240,21
PRIMER_LEFT_41_TM=61.475
PRIMER_RIGHT_41_TM=57.467
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=738
PRIMER_PAIR_42_PENALTY=5.007412
PRIMER_LEFT_42_PENALTY=1.474506
PRIMER_RIGHT_42_PENALTY=3.532907
PRIMER_LEFT_42_SEQUENCE=TGAAAACAATGGGCGTGAGT
PRIMER_RIGHT_42_SEQUENCE=CCTACGCATGTTTACGTTCTC
PRIMER_LEFT_42=492,20
PRIMER_RIGHT_42=1240,21
PRIMER_LEFT_42_TM=61.475
PRIMER_RIGHT_42_TM=57.467
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=749
PRIMER_PAIR_43_PENALTY=5.007892
PRIMER_LEFT_43_PENALTY=3.145499
PRIMER_RIGHT_43_PENALTY=1.862393
PRIMER_LEFT_43_SEQUENCE=AAACAATGGGCGTGAGTTGG
PRIMER_RIGHT_43_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_43=495,20
PRIMER_RIGHT_43=1196,21
PRIMER_LEFT_43_TM=63.145
PRIMER_RIGHT_43_TM=59.138
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=702
PRIMER_PAIR_44_PENALTY=5.031346
PRIMER_LEFT_44_PENALTY=2.063930
PRIMER_RIGHT_44_PENALTY=2.967416
PRIMER_LEFT_44_SEQUENCE=GGGGGAAGGGGTGAAATAAA
PRIMER_RIGHT_44_SEQUENCE=CGAGGCTATAACCCAATACACA
PRIMER_LEFT_44=747,20
PRIMER_RIGHT_44=1594,22
PRIMER_LEFT_44_TM=62.064
PRIMER_RIGHT_44_TM=59.033
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.455
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.2000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=848
PRIMER_PAIR_45_PENALTY=5.036398
PRIMER_LEFT_45_PENALTY=1.375368
PRIMER_RIGHT_45_PENALTY=3.661030
PRIMER_LEFT_45_SEQUENCE=GCGTGAGTTGGAAATTGTGT
PRIMER_RIGHT_45_SEQUENCE=ACCTACGCATGTTTACGTTCTC
PRIMER_LEFT_45=504,20
PRIMER_RIGHT_45=1241,22
PRIMER_LEFT_45_TM=58.625
PRIMER_RIGHT_45_TM=58.339
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=738
PRIMER_PAIR_46_PENALTY=5.098175
PRIMER_LEFT_46_PENALTY=3.235782
PRIMER_RIGHT_46_PENALTY=1.862393
PRIMER_LEFT_46_SEQUENCE=GGCGTGAGTTGGAAATTGTGT
PRIMER_RIGHT_46_SEQUENCE=TATGTGGCTCATCCAGAACAA
PRIMER_LEFT_46=503,21
PRIMER_RIGHT_46=1196,21
PRIMER_LEFT_46_TM=62.236
PRIMER_RIGHT_46_TM=59.138
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=694
PRIMER_PAIR_47_PENALTY=5.112869
PRIMER_LEFT_47_PENALTY=4.202567
PRIMER_RIGHT_47_PENALTY=0.910302
PRIMER_LEFT_47_SEQUENCE=CAATGGGCGTGAGTTGGAAA
PRIMER_RIGHT_47_SEQUENCE=ATGTGGCTCATCCAGAACAA
PRIMER_LEFT_47=498,20
PRIMER_RIGHT_47=1195,20
PRIMER_LEFT_47_TM=64.203
PRIMER_RIGHT_47_TM=59.090
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=698
PRIMER_PAIR_48_PENALTY=5.135535
PRIMER_LEFT_48_PENALTY=1.474506
PRIMER_RIGHT_48_PENALTY=3.661030
PRIMER_LEFT_48_SEQUENCE=GGCGTGAGTTGGAAATTGTG
PRIMER_RIGHT_48_SEQUENCE=ACCTACGCATGTTTACGTTCTC
PRIMER_LEFT_48=503,20
PRIMER_RIGHT_48=1241,22
PRIMER_LEFT_48_TM=61.475
PRIMER_RIGHT_48_TM=58.339
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=739
PRIMER_PAIR_49_PENALTY=5.135535
PRIMER_LEFT_49_PENALTY=1.474506
PRIMER_RIGHT_49_PENALTY=3.661030
PRIMER_LEFT_49_SEQUENCE=TGAAAACAATGGGCGTGAGT
PRIMER_RIGHT_49_SEQUENCE=ACCTACGCATGTTTACGTTCTC
PRIMER_LEFT_49=492,20
PRIMER_RIGHT_49=1241,22
PRIMER_LEFT_49_TM=61.475
PRIMER_RIGHT_49_TM=58.339
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=750
=
SEQUENCE_ID=MAGIC.149_3_9014637_9014649_ct-1_hi-0_65598_1514_TRUE
SEQUENCE_TARGET=950,112
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_9612629_9613227_ct-1_zu-0_307933_12113_TRUE
SEQUENCE_TARGET=950,698
SEQUENCE_TEMPLATE=AGAGAGAGTAGGAGNCNNNNNNNNNNNNNNNNNNNNNNNNGTNTAACTTTGTGANTNTATAATTGNNNNNNNNNNNNNNNNNNNNNNNNAGAGAACTAATNANCAGAGCTACCTCGCCANCTTGNAATCCCNGAAAGAAACCCAGTTCACCTCTTGTGGGTATACAATACACGGAACANNNNNNNNNNNNNNNNNNNNNNNNNNTTATTCTNTGTNNACAACGATTANGACAGGNNGAGNAAATAGGTCGANATATACCCATAAAGTTAGGCAGAATCAATAGCGATCCCCTAGAGANAGACCATGNTGAACGAGNNTTCAAGTATAAGTCCTTCCCAAGCATACATGTAATGTGTAGGTNGACACAACAATANTCATCACACNTATAAAACCCNTCTTCNGGATTTATTTTNCCCTCACAAGCTTCACACCAATANNTCATGCTACTTGTCTTCTTCCCATAANAAAGAGGGAGTATGTGTTTATCATNCNTATACCTNACTTNTTGNGGTAAAGTAGCACATTTNAAACACAAAGCANANTCACACNCNTNCTCAATGCAATTGAAGGTTTCCTTGGTGTAATAACCTCGCGCTTCGGCGCAAACCGAACANTTTCTTTNCTCNTNTNGTNTGGATGTTAGGAATAAAGGATGCNTATGACTTGGATGAAGTAAAGGCTCCGAAATTNTGGCGCACTGCACATGTAGCCTAAAATCGCAATTTCTTTCACTGCACTNATAGAANAAACCAGCTATACACAACGAAGGACAAGCTGAACANNTATCACGATCAGAATTANNNNNNNNNNCGACAGNTCTGAGAGGGTTATCTGTNACTAAAGTGAGTNGATGNGGATGTATCGGGTGATNTNANCTGCGAGAAAGNTTTGCACATTCTTCNTGTAGAATGAAATCACATTGCAANCAAGAGTAGGAGTTACCAAAATAAATNGGCATGATGCATGCTTGACANAGCTTATTCTCGTCGTAATCTTTGNNNGTGTTCTCATNAAGTGTCAAATGATGATNTNCATGACTGAAATNTTGTATGANTCCATCGCTTATNGTCANGAATGGCTCAAGNTCTTCCACTTCTTCTTCNNACTCTCCTTCACGTNCTATACCATCCCAAACATTTGTTTGAGTGGCNCATTTTGAATGNACCGCATAAANACAACCNTTCTTGATGCAATGATANCCCCCGTANTCATTGTTAATCTTCCGGCGGCAAANACCACAAATNCNATCTCCTTTTGGGAAAGAATGGGTAAAANAGATANAATGGNNGTGNCGAGAAATCCGTATGACATGAGGTAAGCTNAAACANCTTTGATGNACGATAAAGTNGCAAGGNGGNCATATATAGAAAANCAANTTTGAATGTTTNAAGGCNCANAGGCTGCAAGTTNNGGNATCCNGACTTGGAAANANAGCAAGTGTATGCNCATGCCACTTGGGTTGGTCGATAGACAAGACTNGTGATTTCTTNGCACATGTAAATTTGAGCCCAAAATCGNAAGNAGAACAAAAATAAAACATACTTGTGGTATCGTCATGGAGAANTTCGTGACAGCAATAGCATTGCNNNANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14472179_14472372_sf-2_ws-0_2189_11940_TRUE
SEQUENCE_TARGET=950,293
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCATNGGTGTTGTNTAGATGAGAACCTATCAACACANNAGGATNAAGNACTTGGTATATTATAATAAAATTATNTGTGNTTTNNNNGAAGAGAATTATGGTCNTTTTACGAATCTTTGTAATGTTTGGTATCTACGATGGTGCATACCGCGAGTTAGCCAAATGTTATGTATTTANTNGGAAACGCGTTTCCATGACGTTTTTTGTGTTTCCAACCTCAGAGCGAATTTCCNTGCNNCCNTNNCNATCGTCNTTGGNATCTGCTCCACCGNCNATTNANATCCCATGCATTCTTGNTGGGAATCANTGTCCAAGCAATNNNNNNNNNNNNTNTCTTGCATCATTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTNTNNTTNTATNTTATTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_8284270_8284959_ws-0_can-0_126238_7759_TRUE
SEQUENCE_TARGET=950,789
SEQUENCE_TEMPLATE=CCCATTNTAGTTAAACTGTTTACGTAAATGNCTCANAAATGTTTTACGNACNTCTNCTCCNNGNAAGCTNGNAAANACGNGGTATCTCCAGGTGCGAGNCGAANNNGANGAAGAANAAGNCATGAGATAACGTGTCAAGAAAAGTTNAAGANCANATATANACANTGNTTCTNAANGCTANGNNATATACGAANAANAAACTNGAAAACGCGTATTCACTATNTTGACTNTTGACTTTTGAGAAANANAGAGANGAAGCTTCTTCGTATTTTCAGTCTCAGCTTTTGATACGTAACGGNNNACATTAAACAAGTTTATTTCTGGGTGANTTTGGTGANTAAAAGACAGNTTGACTAACTNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCATNACAAANATATTTTTGGGCTGACAAGAANNNAACAGTTCAGTCATAATATTTTNNTTTTNNCNANNAAANAATNAAAANNAANTNTNGNATCNCGAGAAAAAAGTTCTATCTAGTTTTGNAAAGANAATANTTTNTATGTTTNCGAANNNNNNNNNNNNAGAATCTCTACGTTNCTTANTNAAAAGGTTANCTACTAGACCAACTCATNGNTGGTTTGGTTTTCATGGGANNTGNNNNNNNAGATTCATGNTTNAAAACAACTNTTAACNNNACANNNNNNNNTNTNCANTATNAGNTTAATNNNNTTTTTTNNNNNNTTTTTNANACNTAATTCATTCATTGATTTTGAATCGAATACAAGNGTTNGATACATCCGTAATACATNANTAATAAACACATTCAAACATACATCCACACAATTNTAATTAAATATTNNAANNANCGANANAAANNTGAATNTCNACNATGCTNTNNAAACCNAAGNCNNNNNNNNNNNNCNTTTCTTTNNTTTTNANANTCAATNCNAAATTGATGANATNGTNGTTNTTGNNTATCATCTCTTNGATGNAANTCCATAATCGTCTAATGTTNTTGATGTTACTTGATATCTTCTCTTGCATCAATTCGTAGATTCTCCNTAGACNNNNNNNNNNNNNATTCCANATTCGTNATACTCCAAATAACTCTNTNAAATNGAGTATNAATGGCTTTCACTTACNGGAANNTTTNATNTNNGGCATCATCAGATTAANANTNTANTCNAAGTATCAGTAGTTATGTTTGTCCTCAAAATTANTACTTTATNTATCAGCTATTTTNNTTTATTAGTANAGAGAACTAGTAAAAAGTTTAANTGGTCGNTGCCNTTAGTGTCAAATCTAAATAANAACAGNAANAAANAATCANTTANAGCTTTTGNACGTCANTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAANAAANTCCATGCNTATTCACTACAAAAATATGCNTAANTNNTTTGAAAANGNNAAAGANCNGGNNACGGGNTAAGAANGAAAACTATTTGGTNCTCCTTANNNNNNATTGGCCAAATAAAAGATTNCAAGCAGAGTTCGGCATANTTTCCCTTANTCTCAGACCAAGCCGACACAATCGCAAAACATGNNNTNNTTTTTGTAACTTATGNGGCTATTNTGTCCTCCGAAGTACCAGTTTGGTTTCNGTCTAATGTAATAGNGGATAAACCGCAAAACANNAGATNGTTNGCATTTNAAACCAACGTTGAAAGAGCCTTTCNNNNNNNNNNNNAATCTTGTAATGCANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTANTGTAAGANAGAANNAAGCNGATAATGTCCNAGACATGTCTTAAAACAANTTGGTGGGTNCNTATGAGGAATATAATGCTATGGTTAANTATANTTTNNTGGAATGTGTNGAGAAATGTNTGATTGATTGNTTGGTTGTTGGTTAAATAAATAATTATTAGCTCAACAGTCCCAATCCAATAATTAGGTTCATTAGTCTTTATCCAATAAAATTATGGGCCTTCCCTTGGTCGGATCTCACATGGTCTTAGCTTCANGGAGCATAACTNACNTCTTTACAGAACTTCAAANCAACTCNNATTCATACATCTTTNAANNNNNNNNNGANGAAATTTAGTTAAAGANAAATTCTTAAGATNTATAANTTTANTTTCAGANTGTATGAAAATAANAAACTTCCTCATGAAAACGCTTTAAGACCTGAAGATGTGCAAAAANAATATGCCCTTTTTTAAGCCANAATCTGAGTTTTTGCCATCCAGAGTAAGCTAAGAGAACCCACTTTTTGTTCCCTACNTTTTANCCAAAAGCAATAGCGNTTTAATATTTTAATCGAANAATGAAAGGCTCGTAGCAAAATATAAAGCATTTNTTGACAATNATACACATTTGGCCAAACCTNTTTTNNNAAAAAAANAAANNNNGTAAGTCTCTGAATGGCTTCGTTGCGGCAAAAGAAGAAGCAAAATCCNGCGAAGAGGGCATGGAAAAACTTTACAAACATGGTCAAGTCGAANTTCAGAGACATGGAGATCGCTTCATCGGTCAGAGAATCAACGGNTNGTGTCNTCCGGTTCATN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_4_10908235_10908277_bur-0_edi-0_304_127489_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=TGCAATAGCATGTCGAAATCGAGCGATGGTGTGCAAGCACGTCCTTCGCCGCAAGATTTGCTTCTTCGAAACGCAGTGGCNGCATCTTGAACGAGCATTAGGTCAAGAACTTGCTCAGTNGTTCTGTCCTTCCAGTTGTGAATAGAGCTATTTCTAGGTGACGTNGCTGCTGAGTTTGGGAGGCTGCAGCTCACAAACTTCGTCTTCATTTGATGATCNNNNNNNNNGTCATCGATGTCTCTCGGACGTGAGACTATCATATCGAAGCTCCCATTCCTNGACAATAGCGATTGCGGATCAACCGAGATCCGCCTCTCGGATACGGATTGCATACTCGTCTTCTTGATAAGAATCTTGGGCTCGTCTCCACCACCTAGAACCTCAAAGTCATGCCAGTCCTCGAGACTACTATCGTCCCTGAAGCTGAGAACATCTGTNNCCATTTGTTNCTCTTTGGCCTGNAANNGAATGAGGACTAATAAGAAAATGAAAATATTGGCAAAANGNCTAANACAGATTGATTGATACTTAAATACGGAGCAGGATGGGTTGCACGTCGGTGNCTACNTTAGTCATGACTTGCACGTTTAATATAATATAACCCAAAGTGTTGTTGACTTCATCTNCTAAAAAANGAAAAATGNNTCATNNTTTTTTTATTNGTTAANTATCNCANTATCGTTGACATGAGACCATNCCCANGATCAAGACAAGATCAAAAAGATAGATCTGCCTGCACGAACTACCTAATTATAATATTACCAAAGNATTTGGAGGGTNGTAATCTGCATCAGTGAAGACCATAAGTAGAAGCCATAAATCTTGAATACNTCTNCNNNTNNATGNACNTNGTNCTNTATNNNNTACNTNNTACTTGGNNANTTNTTTNTNNTTGGTNACNTNTNNTTNTCNTTGNTNACTNTNCNCGNNANACTTNGNTATANNNCTTNNTNATANNNCTTNTNNNTNNNTCTNNTATTTTTNNNTTNNNNTNNNTTTTNCNNNNNNNNNNNNNNNNNCTTTGAATTTTNNTGTGTCTTTTNCTACAAATATTTCTTCACTTTCATTGAAATTCATCNCCTTCAATGGATGCACNTACGTCCGTTAGTCATNNNTGGCTTTAGGCCTCATATNTATTCTAGNTGCTCTAGTATATCCCTCNAAAATGGACGGTGAGAAACAATACCAATTTTTNATCCCAAGGGATTTGAACCGGTTATGTTTGAATAACATAGNGATTTGAACGTCAAATCAACAANTTACGAAACGATTAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGCTAAAACATTTTTGAANGTTTTCAAATATGGATTTGATCGACAAANATAGAAGTCATATACTCCTTGATTTTTCCTTGGGGATTGGTGNATCCTTCATTTNCATTGTAGGNGACGNTCATCATNCGTGNAAGATCAATGGTTGCCTTAAGAAGAAAATGTGGCACGCCAGTTGTTGNCAAGAACTCCTCATTTAGTGTCTTATTGGCANCTNCAACNATTTTANNAAGCTCCCTAAAAGNTTCCTCTTTGGTAACCCCATATTGCTTCATATANCAGTTNACCGCGTTCGNGANATATCCTCTACTCATGTCNTCCTACTCAATATTTGNGTAAGTNGGTAAGATTTACAAGAAAGCAGTTATACGCGAGATGTAAAGAAANNATTTATGTATTTGTACCTCATAACCCATNAAATCATTNATAAGACGACCTTTGATAGACAAATATTGGACGAGTTTTGGTCTGGATTTCAGCCNNTCAAAAGCTTCCTTTGTNGCCATCTTTCCNATAGACATGAAAATNCCTGCCAAGGACGCATACACTGTGACCTCCACCTCACCAACCTCCATATACTNCTCAAAGCTAGGAATGTGATCNGCTTGTGNCCATTTTTCGAGATCAATGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.350503
PRIMER_LEFT_0_PENALTY=0.083883
PRIMER_RIGHT_0_PENALTY=0.266620
PRIMER_LEFT_0_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_0_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_0=529,20
PRIMER_RIGHT_0=1500,20
PRIMER_LEFT_0_TM=59.916
PRIMER_RIGHT_0_TM=59.733
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=972
PRIMER_PAIR_1_PENALTY=0.541950
PRIMER_LEFT_1_PENALTY=0.285121
PRIMER_RIGHT_1_PENALTY=0.256829
PRIMER_LEFT_1_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_1_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_1=727,20
PRIMER_RIGHT_1=1582,20
PRIMER_LEFT_1_TM=60.285
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=0.551741
PRIMER_LEFT_2_PENALTY=0.285121
PRIMER_RIGHT_2_PENALTY=0.266620
PRIMER_LEFT_2_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_2_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_2=727,20
PRIMER_RIGHT_2=1500,20
PRIMER_LEFT_2_TM=60.285
PRIMER_RIGHT_2_TM=59.733
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=774
PRIMER_PAIR_3_PENALTY=0.566115
PRIMER_LEFT_3_PENALTY=0.083883
PRIMER_RIGHT_3_PENALTY=0.482233
PRIMER_LEFT_3_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_3_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_3=529,20
PRIMER_RIGHT_3=1501,20
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=60.482
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=973
PRIMER_PAIR_4_PENALTY=0.767353
PRIMER_LEFT_4_PENALTY=0.285121
PRIMER_RIGHT_4_PENALTY=0.482233
PRIMER_LEFT_4_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_4_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_4=727,20
PRIMER_RIGHT_4=1501,20
PRIMER_LEFT_4_TM=60.285
PRIMER_RIGHT_4_TM=60.482
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=775
PRIMER_PAIR_5_PENALTY=0.769116
PRIMER_LEFT_5_PENALTY=0.512286
PRIMER_RIGHT_5_PENALTY=0.256829
PRIMER_LEFT_5_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_5_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_5=730,20
PRIMER_RIGHT_5=1582,20
PRIMER_LEFT_5_TM=59.488
PRIMER_RIGHT_5_TM=60.257
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.5000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=853
PRIMER_PAIR_6_PENALTY=0.778907
PRIMER_LEFT_6_PENALTY=0.512286
PRIMER_RIGHT_6_PENALTY=0.266620
PRIMER_LEFT_6_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_6_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_6=730,20
PRIMER_RIGHT_6=1500,20
PRIMER_LEFT_6_TM=59.488
PRIMER_RIGHT_6_TM=59.733
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=771
PRIMER_PAIR_7_PENALTY=0.979374
PRIMER_LEFT_7_PENALTY=0.712754
PRIMER_RIGHT_7_PENALTY=0.266620
PRIMER_LEFT_7_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_7_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_7=531,20
PRIMER_RIGHT_7=1500,20
PRIMER_LEFT_7_TM=60.713
PRIMER_RIGHT_7_TM=59.733
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=970
PRIMER_PAIR_8_PENALTY=0.979374
PRIMER_LEFT_8_PENALTY=0.712754
PRIMER_RIGHT_8_PENALTY=0.266620
PRIMER_LEFT_8_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_8_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_8=530,20
PRIMER_RIGHT_8=1500,20
PRIMER_LEFT_8_TM=60.713
PRIMER_RIGHT_8_TM=59.733
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=0.994519
PRIMER_LEFT_9_PENALTY=0.512286
PRIMER_RIGHT_9_PENALTY=0.482233
PRIMER_LEFT_9_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_9_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_9=730,20
PRIMER_RIGHT_9=1501,20
PRIMER_LEFT_9_TM=59.488
PRIMER_RIGHT_9_TM=60.482
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.5000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=772
PRIMER_PAIR_10_PENALTY=1.194986
PRIMER_LEFT_10_PENALTY=0.712754
PRIMER_RIGHT_10_PENALTY=0.482233
PRIMER_LEFT_10_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_10_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_10=531,20
PRIMER_RIGHT_10=1501,20
PRIMER_LEFT_10_TM=60.713
PRIMER_RIGHT_10_TM=60.482
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=971
PRIMER_PAIR_11_PENALTY=1.194986
PRIMER_LEFT_11_PENALTY=0.712754
PRIMER_RIGHT_11_PENALTY=0.482233
PRIMER_LEFT_11_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_11_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_11=530,20
PRIMER_RIGHT_11=1501,20
PRIMER_LEFT_11_TM=60.713
PRIMER_RIGHT_11_TM=60.482
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=1.227016
PRIMER_LEFT_12_PENALTY=0.083883
PRIMER_RIGHT_12_PENALTY=1.143133
PRIMER_LEFT_12_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_12_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_12=529,20
PRIMER_RIGHT_12=1496,20
PRIMER_LEFT_12_TM=59.916
PRIMER_RIGHT_12_TM=61.143
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=968
PRIMER_PAIR_13_PENALTY=1.227016
PRIMER_LEFT_13_PENALTY=0.083883
PRIMER_RIGHT_13_PENALTY=1.143133
PRIMER_LEFT_13_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_13_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_13=529,20
PRIMER_RIGHT_13=1497,20
PRIMER_LEFT_13_TM=59.916
PRIMER_RIGHT_13_TM=61.143
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=969
PRIMER_PAIR_14_PENALTY=1.239333
PRIMER_LEFT_14_PENALTY=0.083883
PRIMER_RIGHT_14_PENALTY=1.155450
PRIMER_LEFT_14_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_14_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_14=529,20
PRIMER_RIGHT_14=1186,21
PRIMER_LEFT_14_TM=59.916
PRIMER_RIGHT_14_TM=59.845
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=658
PRIMER_PAIR_15_PENALTY=1.239333
PRIMER_LEFT_15_PENALTY=0.083883
PRIMER_RIGHT_15_PENALTY=1.155450
PRIMER_LEFT_15_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_15_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_15=529,20
PRIMER_RIGHT_15=1187,21
PRIMER_LEFT_15_TM=59.916
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=659
PRIMER_PAIR_16_PENALTY=1.252490
PRIMER_LEFT_16_PENALTY=0.995660
PRIMER_RIGHT_16_PENALTY=0.256829
PRIMER_LEFT_16_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_16_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_16=728,20
PRIMER_RIGHT_16=1582,20
PRIMER_LEFT_16_TM=60.996
PRIMER_RIGHT_16_TM=60.257
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=855
PRIMER_PAIR_17_PENALTY=1.262281
PRIMER_LEFT_17_PENALTY=0.995660
PRIMER_RIGHT_17_PENALTY=0.266620
PRIMER_LEFT_17_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_17_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_17=728,20
PRIMER_RIGHT_17=1500,20
PRIMER_LEFT_17_TM=60.996
PRIMER_RIGHT_17_TM=59.733
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=773
PRIMER_PAIR_18_PENALTY=1.428253
PRIMER_LEFT_18_PENALTY=0.285121
PRIMER_RIGHT_18_PENALTY=1.143133
PRIMER_LEFT_18_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_18_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_18=727,20
PRIMER_RIGHT_18=1496,20
PRIMER_LEFT_18_TM=60.285
PRIMER_RIGHT_18_TM=61.143
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=770
PRIMER_PAIR_19_PENALTY=1.428253
PRIMER_LEFT_19_PENALTY=0.285121
PRIMER_RIGHT_19_PENALTY=1.143133
PRIMER_LEFT_19_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_19_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_19=727,20
PRIMER_RIGHT_19=1497,20
PRIMER_LEFT_19_TM=60.285
PRIMER_RIGHT_19_TM=61.143
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=771
PRIMER_PAIR_20_PENALTY=1.440571
PRIMER_LEFT_20_PENALTY=0.285121
PRIMER_RIGHT_20_PENALTY=1.155450
PRIMER_LEFT_20_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_20_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_20=727,20
PRIMER_RIGHT_20=1186,21
PRIMER_LEFT_20_TM=60.285
PRIMER_RIGHT_20_TM=59.845
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=460
PRIMER_PAIR_21_PENALTY=1.440571
PRIMER_LEFT_21_PENALTY=0.285121
PRIMER_RIGHT_21_PENALTY=1.155450
PRIMER_LEFT_21_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_21_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_21=727,20
PRIMER_RIGHT_21=1187,21
PRIMER_LEFT_21_TM=60.285
PRIMER_RIGHT_21_TM=59.845
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=461
PRIMER_PAIR_22_PENALTY=1.465064
PRIMER_LEFT_22_PENALTY=0.083883
PRIMER_RIGHT_22_PENALTY=1.381181
PRIMER_LEFT_22_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_22_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_22=529,20
PRIMER_RIGHT_22=1495,20
PRIMER_LEFT_22_TM=59.916
PRIMER_RIGHT_22_TM=58.619
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=967
PRIMER_PAIR_23_PENALTY=1.477893
PRIMER_LEFT_23_PENALTY=0.995660
PRIMER_RIGHT_23_PENALTY=0.482233
PRIMER_LEFT_23_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_23_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_23=728,20
PRIMER_RIGHT_23=1501,20
PRIMER_LEFT_23_TM=60.996
PRIMER_RIGHT_23_TM=60.482
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=774
PRIMER_PAIR_24_PENALTY=1.581829
PRIMER_LEFT_24_PENALTY=0.426379
PRIMER_RIGHT_24_PENALTY=1.155450
PRIMER_LEFT_24_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_24_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_24=361,20
PRIMER_RIGHT_24=1186,21
PRIMER_LEFT_24_TM=59.574
PRIMER_RIGHT_24_TM=59.845
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=826
PRIMER_PAIR_25_PENALTY=1.581829
PRIMER_LEFT_25_PENALTY=0.426379
PRIMER_RIGHT_25_PENALTY=1.155450
PRIMER_LEFT_25_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_25_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_25=361,20
PRIMER_RIGHT_25=1187,21
PRIMER_LEFT_25_TM=59.574
PRIMER_RIGHT_25_TM=59.845
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=827
PRIMER_PAIR_26_PENALTY=1.637322
PRIMER_LEFT_26_PENALTY=0.083883
PRIMER_RIGHT_26_PENALTY=1.553439
PRIMER_LEFT_26_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_26_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_26=529,20
PRIMER_RIGHT_26=1495,21
PRIMER_LEFT_26_TM=59.916
PRIMER_RIGHT_26_TM=59.447
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=967
PRIMER_PAIR_27_PENALTY=1.655419
PRIMER_LEFT_27_PENALTY=0.512286
PRIMER_RIGHT_27_PENALTY=1.143133
PRIMER_LEFT_27_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_27_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_27=730,20
PRIMER_RIGHT_27=1496,20
PRIMER_LEFT_27_TM=59.488
PRIMER_RIGHT_27_TM=61.143
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.5000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=767
PRIMER_PAIR_28_PENALTY=1.655419
PRIMER_LEFT_28_PENALTY=0.512286
PRIMER_RIGHT_28_PENALTY=1.143133
PRIMER_LEFT_28_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_28_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_28=730,20
PRIMER_RIGHT_28=1497,20
PRIMER_LEFT_28_TM=59.488
PRIMER_RIGHT_28_TM=61.143
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.5000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=768
PRIMER_PAIR_29_PENALTY=1.666302
PRIMER_LEFT_29_PENALTY=0.285121
PRIMER_RIGHT_29_PENALTY=1.381181
PRIMER_LEFT_29_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_29_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_29=727,20
PRIMER_RIGHT_29=1495,20
PRIMER_LEFT_29_TM=60.285
PRIMER_RIGHT_29_TM=58.619
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=769
PRIMER_PAIR_30_PENALTY=1.667737
PRIMER_LEFT_30_PENALTY=0.512286
PRIMER_RIGHT_30_PENALTY=1.155450
PRIMER_LEFT_30_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_30_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_30=730,20
PRIMER_RIGHT_30=1186,21
PRIMER_LEFT_30_TM=59.488
PRIMER_RIGHT_30_TM=59.845
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.5000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=457
PRIMER_PAIR_31_PENALTY=1.667737
PRIMER_LEFT_31_PENALTY=0.512286
PRIMER_RIGHT_31_PENALTY=1.155450
PRIMER_LEFT_31_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_31_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_31=730,20
PRIMER_RIGHT_31=1187,21
PRIMER_LEFT_31_TM=59.488
PRIMER_RIGHT_31_TM=59.845
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=458
PRIMER_PAIR_32_PENALTY=1.669226
PRIMER_LEFT_32_PENALTY=0.513776
PRIMER_RIGHT_32_PENALTY=1.155450
PRIMER_LEFT_32_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_32_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_32=257,20
PRIMER_RIGHT_32=1186,21
PRIMER_LEFT_32_TM=59.486
PRIMER_RIGHT_32_TM=59.845
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=930
PRIMER_PAIR_33_PENALTY=1.669226
PRIMER_LEFT_33_PENALTY=0.513776
PRIMER_RIGHT_33_PENALTY=1.155450
PRIMER_LEFT_33_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_33_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_33=257,20
PRIMER_RIGHT_33=1187,21
PRIMER_LEFT_33_TM=59.486
PRIMER_RIGHT_33_TM=59.845
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=1.703379
PRIMER_LEFT_34_PENALTY=1.446550
PRIMER_RIGHT_34_PENALTY=0.256829
PRIMER_LEFT_34_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_34_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_34=729,21
PRIMER_RIGHT_34=1582,20
PRIMER_LEFT_34_TM=60.447
PRIMER_RIGHT_34_TM=60.257
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=854
PRIMER_PAIR_35_PENALTY=1.713170
PRIMER_LEFT_35_PENALTY=1.446550
PRIMER_RIGHT_35_PENALTY=0.266620
PRIMER_LEFT_35_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_35_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_35=729,21
PRIMER_RIGHT_35=1500,20
PRIMER_LEFT_35_TM=60.447
PRIMER_RIGHT_35_TM=59.733
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.5000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=772
PRIMER_PAIR_36_PENALTY=1.740964
PRIMER_LEFT_36_PENALTY=1.484135
PRIMER_RIGHT_36_PENALTY=0.256829
PRIMER_LEFT_36_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_36_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_36=725,21
PRIMER_RIGHT_36=1582,20
PRIMER_LEFT_36_TM=59.516
PRIMER_RIGHT_36_TM=60.257
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.1000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=858
PRIMER_PAIR_37_PENALTY=1.750755
PRIMER_LEFT_37_PENALTY=1.484135
PRIMER_RIGHT_37_PENALTY=0.266620
PRIMER_LEFT_37_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_37_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_37=725,21
PRIMER_RIGHT_37=1500,20
PRIMER_LEFT_37_TM=59.516
PRIMER_RIGHT_37_TM=59.733
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.1000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=776
PRIMER_PAIR_38_PENALTY=1.785200
PRIMER_LEFT_38_PENALTY=1.528370
PRIMER_RIGHT_38_PENALTY=0.256829
PRIMER_LEFT_38_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_38_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_38=726,20
PRIMER_RIGHT_38=1582,20
PRIMER_LEFT_38_TM=58.472
PRIMER_RIGHT_38_TM=60.257
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.1000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=857
PRIMER_PAIR_39_PENALTY=1.794991
PRIMER_LEFT_39_PENALTY=1.528370
PRIMER_RIGHT_39_PENALTY=0.266620
PRIMER_LEFT_39_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_39_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_39=726,20
PRIMER_RIGHT_39=1500,20
PRIMER_LEFT_39_TM=58.472
PRIMER_RIGHT_39_TM=59.733
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.1000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=775
PRIMER_PAIR_40_PENALTY=1.811843
PRIMER_LEFT_40_PENALTY=0.656393
PRIMER_RIGHT_40_PENALTY=1.155450
PRIMER_LEFT_40_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_40_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_40=379,20
PRIMER_RIGHT_40=1186,21
PRIMER_LEFT_40_TM=60.656
PRIMER_RIGHT_40_TM=59.845
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=808
PRIMER_PAIR_41_PENALTY=1.811843
PRIMER_LEFT_41_PENALTY=0.656393
PRIMER_RIGHT_41_PENALTY=1.155450
PRIMER_LEFT_41_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_41_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_41=379,20
PRIMER_RIGHT_41=1187,21
PRIMER_LEFT_41_TM=60.656
PRIMER_RIGHT_41_TM=59.845
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=809
PRIMER_PAIR_42_PENALTY=1.838560
PRIMER_LEFT_42_PENALTY=0.285121
PRIMER_RIGHT_42_PENALTY=1.553439
PRIMER_LEFT_42_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_42_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_42=727,20
PRIMER_RIGHT_42=1495,21
PRIMER_LEFT_42_TM=60.285
PRIMER_RIGHT_42_TM=59.447
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=769
PRIMER_PAIR_43_PENALTY=1.855887
PRIMER_LEFT_43_PENALTY=0.712754
PRIMER_RIGHT_43_PENALTY=1.143133
PRIMER_LEFT_43_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_43_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_43=531,20
PRIMER_RIGHT_43=1496,20
PRIMER_LEFT_43_TM=60.713
PRIMER_RIGHT_43_TM=61.143
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=1.855887
PRIMER_LEFT_44_PENALTY=0.712754
PRIMER_RIGHT_44_PENALTY=1.143133
PRIMER_LEFT_44_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_44_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_44=531,20
PRIMER_RIGHT_44=1497,20
PRIMER_LEFT_44_TM=60.713
PRIMER_RIGHT_44_TM=61.143
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=967
PRIMER_PAIR_45_PENALTY=1.855887
PRIMER_LEFT_45_PENALTY=0.712754
PRIMER_RIGHT_45_PENALTY=1.143133
PRIMER_LEFT_45_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_45_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_45=530,20
PRIMER_RIGHT_45=1496,20
PRIMER_LEFT_45_TM=60.713
PRIMER_RIGHT_45_TM=61.143
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=967
PRIMER_PAIR_46_PENALTY=1.855887
PRIMER_LEFT_46_PENALTY=0.712754
PRIMER_RIGHT_46_PENALTY=1.143133
PRIMER_LEFT_46_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_46_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_46=530,20
PRIMER_RIGHT_46=1497,20
PRIMER_LEFT_46_TM=60.713
PRIMER_RIGHT_46_TM=61.143
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=968
PRIMER_PAIR_47_PENALTY=1.868204
PRIMER_LEFT_47_PENALTY=0.712754
PRIMER_RIGHT_47_PENALTY=1.155450
PRIMER_LEFT_47_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_47_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_47=531,20
PRIMER_RIGHT_47=1186,21
PRIMER_LEFT_47_TM=60.713
PRIMER_RIGHT_47_TM=59.845
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=656
PRIMER_PAIR_48_PENALTY=1.868204
PRIMER_LEFT_48_PENALTY=0.712754
PRIMER_RIGHT_48_PENALTY=1.155450
PRIMER_LEFT_48_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_48_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_48=531,20
PRIMER_RIGHT_48=1187,21
PRIMER_LEFT_48_TM=60.713
PRIMER_RIGHT_48_TM=59.845
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=657
PRIMER_PAIR_49_PENALTY=1.868204
PRIMER_LEFT_49_PENALTY=0.712754
PRIMER_RIGHT_49_PENALTY=1.155450
PRIMER_LEFT_49_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_49_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_49=530,20
PRIMER_RIGHT_49=1186,21
PRIMER_LEFT_49_TM=60.713
PRIMER_RIGHT_49_TM=59.845
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=657
=
SEQUENCE_ID=MAGIC.149_3_13971386_13971551_ct-1_sf-2_51265_19595_TRUE
SEQUENCE_TARGET=950,265
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_18458482_18459107_no-0_hi-0_66569_126309_TRUE
SEQUENCE_TARGET=950,725
SEQUENCE_TEMPLATE=GTAAGATTCTTTACCTTAAAGCATCGAACTTTGTGGCTTATGTGTTCCCATATGTCAACAACGGACCTCTATCGTCCACAATACACACAAATGCAGCATAAGTTANTTNAATTATCGCCGGAATAAAGTTCTATAATTATTTGATTTGTTTTTCTTTTTTGGAATTTCCTCGGCAGGAATACATTTTCAAATTTCNTCAAATGATATTTTGNTTTGTAGTTGAAACATATTAGATTTTATTTTCCGATCCAAATTTTGTTCCCANCATGTCTTTTTGAATCATTTTACAAGAATGANTTAAGTATAAAAAAGGAGAATTAACATTANGTTTAATTTCTTAAAAAATCNTTNACAGAAGAGATCAATTATCTTTCCGCAAGTTCTCTGAAACTGTTTAATAAATGAATTTTATACTTTATCAGCATGCAAACCTTATTTTTANAATGACCAAAAACACTATCAGCAAAAGACTAATAATTTACATTCTTCATNAGAAATTTAAAGATGAGAGAATACATGGAAACTTCTCCGATTGGTCAATGACAAAGGTTTTTGAAAGTTCNAAAAAAAAAAAAAGACATAAACCAGTCAGACCAAAACATGGAGGAAGAGAAACAAACCCTAGAGACGATTCTCTCCGCAACTGTTAAAAATTTCAGTTGCAGAGAGATTAATATAAGACACTGAAATTGAAAGACAAACTCCTAAAAGAAAAAGTCTTCTCTTTGCTTTGTTTAAGATTNGGACAATTTGCTAATCGCATCCCAATCAATCTCCACAGAAGGAAACTTCTCCAGCCCGAAACTCCCAATCTCATCAAATCCAGAATCCGAAAAGTCCAAGAACGTAGTAATACCAGATTCCGGCGAAGAAGATTCCACATGAGACTCATCTGAAAACGAATTCTCATCAGATCTCAAGAACCCATATTCACTTTCTTGATACTCTGTTTTTGGCGGGAAAAGCTCTGTTTCNGAACNGGGGAGNTCAANATNCTCTGNTTTTCTCANGCTCTGACAAATCTCTTGGAGCTTTGCGTCNACAGAGGAATGAAGAGGATTGAAACAGCTACCTCCTNCGTAGTATNCATCCTCGTGTCTGAACTGTGGGAAATTAAGTCTCGCGAACTCNCCTCTTAGCTTGTAAGCAGCTAGGTCATAAGCCATGGCTGCTTCTTCAGCTGTNTCGAAAGTTCCAAGCCAGAGTCGGGTCCGGTTCTTGGGTAAACGGATCTCAGCTACCCATTTTCCCCAGTGCCTTTGTCTAACTCCTCTATAGAGTTTCTGAGCGGTCATGTTCTTCATTGGGACNGGCTTTGGGGCTAGATTTGGAGAGATTATGTTACGTCTCTGGTTAAGCTGGTTTTGGATTTGGTGGATTTGGTAAGGTGTGAGCTGGTTTAGACCGAGAGATGTGGTTTGATTGAAACCGAAGACTGNGTTTGAATACGTAGGAGAAGAAGAATCANTGTTGGTGGTATAAGGTTGAAGTGCTTTCATGAGCTCTTCNTGAAAAGGATCTGTGTTGCTATTGAACATATCTATGGCTGCCATGGATCTTTAAAGACTAGGAAACAAAATTAAAACAGAGAGGGATTAAAAACACACCCTCTTAGTCGATTGAATNGAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTATAGCATTTTTTGTTTCTAAATATTTGTTTTTTTCTGTCGCTTTTGGTTTCGCTTGATGCCAAGTTCTTTGCTTATATAACNCCATAAGCTAAGCCGCCCATTGAGATCATTATCATTTTAAAANTATATATAAATTTCTTCAGTAGAATGTTAAATTTAATAATTACATTGTTTAGATGCTTTTGTTCTGTTNACGATTTGTTTCTCGTCAGCTTGATAAAAATCTTTTATTCCATGGATCTAAGCCGTTCCCAGGGAATATAATACGATTTTATGGTGTGTACGTGTGTGACAAAACAATCANCTATCATGGTTAGACTAAGAGTTCTAAATTTTACAAAAATTCCCAGCTCAANTTTTNNNNAAAAATCATAACTATACTAGCTCGCAATAACTATAAATATCAATTTCACTTTCTATTGTGTTTATTAAGGATATATTTATATTGATTTCATGNTATATGTGANNAGCGTGTTATATTTNTNTTTGAAAANGAGAGCATGTTATAGTTTAATNAGGTTTACACGTNACACACACACCAAAAAGACGGAATTAATTTTCTTTAACTTCTCANATGATCTTTGACTNTAGTTTTGGAGCTATATTNAATANATAAAATCGCCGAAATANAATCTTTTCAGCAATANTTTCAAAANTCGTAAGAGNCAAAAACTGACATTAGTTGATTCTANNGCAAGTTGAAAGGCCAGTTTGCCTTCTTTACCACTTCTTTTCTTTTGCCAACATATCTTCTTATAAAATAATTTATATTTTACTCATGTTGAATTAGAAGGNAAAAAAANAAGCATGGAATCAGTACTTGAAGTACTAAATAATATTCATGCAAACAAAAAGAAGAAATTGCAGCCATATCAAACATGAGAATATTGGTGGTCATACTAATATTCCCAAGTCCTAATGTGGTCCATTTTCAGAAATTTTTATTGANTATACCGCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.298895
PRIMER_LEFT_0_PENALTY=0.044122
PRIMER_RIGHT_0_PENALTY=0.254773
PRIMER_LEFT_0_SEQUENCE=CCAGAATCCGAAAAGTCCAA
PRIMER_RIGHT_0_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_0=822,20
PRIMER_RIGHT_0=1779,20
PRIMER_LEFT_0_TM=60.044
PRIMER_RIGHT_0_TM=59.745
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=12.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=958
PRIMER_PAIR_1_PENALTY=0.300952
PRIMER_LEFT_1_PENALTY=0.044122
PRIMER_RIGHT_1_PENALTY=0.256829
PRIMER_LEFT_1_SEQUENCE=CCAGAATCCGAAAAGTCCAA
PRIMER_RIGHT_1_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_1=822,20
PRIMER_RIGHT_1=1731,20
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=910
PRIMER_PAIR_2_PENALTY=0.332113
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.254773
PRIMER_LEFT_2_SEQUENCE=GTAATACCAGATTCCGGCGA
PRIMER_RIGHT_2_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_2=849,20
PRIMER_RIGHT_2=1779,20
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=59.745
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=931
PRIMER_PAIR_3_PENALTY=0.334170
PRIMER_LEFT_3_PENALTY=0.077340
PRIMER_RIGHT_3_PENALTY=0.256829
PRIMER_LEFT_3_SEQUENCE=GTAATACCAGATTCCGGCGA
PRIMER_RIGHT_3_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_3=849,20
PRIMER_RIGHT_3=1731,20
PRIMER_LEFT_3_TM=59.923
PRIMER_RIGHT_3_TM=60.257
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=883
PRIMER_PAIR_4_PENALTY=0.396088
PRIMER_LEFT_4_PENALTY=0.139259
PRIMER_RIGHT_4_PENALTY=0.256829
PRIMER_LEFT_4_SEQUENCE=ATCGCATCCCAATCAATCTC
PRIMER_RIGHT_4_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_4=756,20
PRIMER_RIGHT_4=1731,20
PRIMER_LEFT_4_TM=59.861
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=976
PRIMER_PAIR_5_PENALTY=0.447111
PRIMER_LEFT_5_PENALTY=0.190281
PRIMER_RIGHT_5_PENALTY=0.256829
PRIMER_LEFT_5_SEQUENCE=GAAGGAAACTTCTCCAGCCC
PRIMER_RIGHT_5_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_5=780,20
PRIMER_RIGHT_5=1731,20
PRIMER_LEFT_5_TM=60.190
PRIMER_RIGHT_5_TM=60.257
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=10.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=952
PRIMER_PAIR_6_PENALTY=0.546267
PRIMER_LEFT_6_PENALTY=0.289438
PRIMER_RIGHT_6_PENALTY=0.256829
PRIMER_LEFT_6_SEQUENCE=GCATCCCAATCAATCTCCAC
PRIMER_RIGHT_6_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_6=759,20
PRIMER_RIGHT_6=1731,20
PRIMER_LEFT_6_TM=60.289
PRIMER_RIGHT_6_TM=60.257
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=973
PRIMER_PAIR_7_PENALTY=0.680422
PRIMER_LEFT_7_PENALTY=0.423592
PRIMER_RIGHT_7_PENALTY=0.256829
PRIMER_LEFT_7_SEQUENCE=CAATTTGCTAATCGCATCCC
PRIMER_RIGHT_7_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_7=746,20
PRIMER_RIGHT_7=1731,20
PRIMER_LEFT_7_TM=60.424
PRIMER_RIGHT_7_TM=60.257
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.3000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=986
PRIMER_PAIR_8_PENALTY=0.771448
PRIMER_LEFT_8_PENALTY=0.516675
PRIMER_RIGHT_8_PENALTY=0.254773
PRIMER_LEFT_8_SEQUENCE=CCGAAACTCCCAATCTCATC
PRIMER_RIGHT_8_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_8=798,20
PRIMER_RIGHT_8=1779,20
PRIMER_LEFT_8_TM=59.483
PRIMER_RIGHT_8_TM=59.745
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=12.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=982
PRIMER_PAIR_9_PENALTY=0.773505
PRIMER_LEFT_9_PENALTY=0.516675
PRIMER_RIGHT_9_PENALTY=0.256829
PRIMER_LEFT_9_SEQUENCE=CCGAAACTCCCAATCTCATC
PRIMER_RIGHT_9_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_9=798,20
PRIMER_RIGHT_9=1731,20
PRIMER_LEFT_9_TM=59.483
PRIMER_RIGHT_9_TM=60.257
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=934
PRIMER_PAIR_10_PENALTY=0.796132
PRIMER_LEFT_10_PENALTY=0.044122
PRIMER_RIGHT_10_PENALTY=0.752010
PRIMER_LEFT_10_SEQUENCE=CCAGAATCCGAAAAGTCCAA
PRIMER_RIGHT_10_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_10=822,20
PRIMER_RIGHT_10=1725,20
PRIMER_LEFT_10_TM=60.044
PRIMER_RIGHT_10_TM=60.752
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=2.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=904
PRIMER_PAIR_11_PENALTY=0.826971
PRIMER_LEFT_11_PENALTY=0.572198
PRIMER_RIGHT_11_PENALTY=0.254773
PRIMER_LEFT_11_SEQUENCE=TCCAGAATCCGAAAAGTCCA
PRIMER_RIGHT_11_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_11=821,20
PRIMER_RIGHT_11=1779,20
PRIMER_LEFT_11_TM=60.572
PRIMER_RIGHT_11_TM=59.745
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=12.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=959
PRIMER_PAIR_12_PENALTY=0.829027
PRIMER_LEFT_12_PENALTY=0.572198
PRIMER_RIGHT_12_PENALTY=0.256829
PRIMER_LEFT_12_SEQUENCE=TCCAGAATCCGAAAAGTCCA
PRIMER_RIGHT_12_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_12=821,20
PRIMER_RIGHT_12=1731,20
PRIMER_LEFT_12_TM=60.572
PRIMER_RIGHT_12_TM=60.257
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=911
PRIMER_PAIR_13_PENALTY=0.829350
PRIMER_LEFT_13_PENALTY=0.077340
PRIMER_RIGHT_13_PENALTY=0.752010
PRIMER_LEFT_13_SEQUENCE=GTAATACCAGATTCCGGCGA
PRIMER_RIGHT_13_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_13=849,20
PRIMER_RIGHT_13=1725,20
PRIMER_LEFT_13_TM=59.923
PRIMER_RIGHT_13_TM=60.752
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=877
PRIMER_PAIR_14_PENALTY=0.864649
PRIMER_LEFT_14_PENALTY=0.609876
PRIMER_RIGHT_14_PENALTY=0.254773
PRIMER_LEFT_14_SEQUENCE=AAACTTCTCCAGCCCGAAAC
PRIMER_RIGHT_14_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_14=785,20
PRIMER_RIGHT_14=1779,20
PRIMER_LEFT_14_TM=60.610
PRIMER_RIGHT_14_TM=59.745
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=12.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=995
PRIMER_PAIR_15_PENALTY=0.866705
PRIMER_LEFT_15_PENALTY=0.609876
PRIMER_RIGHT_15_PENALTY=0.256829
PRIMER_LEFT_15_SEQUENCE=AAACTTCTCCAGCCCGAAAC
PRIMER_RIGHT_15_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_15=785,20
PRIMER_RIGHT_15=1731,20
PRIMER_LEFT_15_TM=60.610
PRIMER_RIGHT_15_TM=60.257
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=947
PRIMER_PAIR_16_PENALTY=0.891269
PRIMER_LEFT_16_PENALTY=0.139259
PRIMER_RIGHT_16_PENALTY=0.752010
PRIMER_LEFT_16_SEQUENCE=ATCGCATCCCAATCAATCTC
PRIMER_RIGHT_16_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_16=756,20
PRIMER_RIGHT_16=1725,20
PRIMER_LEFT_16_TM=59.861
PRIMER_RIGHT_16_TM=60.752
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.3000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=970
PRIMER_PAIR_17_PENALTY=0.908717
PRIMER_LEFT_17_PENALTY=0.651888
PRIMER_RIGHT_17_PENALTY=0.256829
PRIMER_LEFT_17_SEQUENCE=AATCGCATCCCAATCAATCT
PRIMER_RIGHT_17_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_17=755,20
PRIMER_RIGHT_17=1731,20
PRIMER_LEFT_17_TM=59.348
PRIMER_RIGHT_17_TM=60.257
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=977
PRIMER_PAIR_18_PENALTY=0.942291
PRIMER_LEFT_18_PENALTY=0.190281
PRIMER_RIGHT_18_PENALTY=0.752010
PRIMER_LEFT_18_SEQUENCE=GAAGGAAACTTCTCCAGCCC
PRIMER_RIGHT_18_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_18=780,20
PRIMER_RIGHT_18=1725,20
PRIMER_LEFT_18_TM=60.190
PRIMER_RIGHT_18_TM=60.752
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=10.9000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=946
PRIMER_PAIR_19_PENALTY=0.961033
PRIMER_LEFT_19_PENALTY=0.704204
PRIMER_RIGHT_19_PENALTY=0.256829
PRIMER_LEFT_19_SEQUENCE=CATCCCAATCAATCTCCACA
PRIMER_RIGHT_19_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_19=760,20
PRIMER_RIGHT_19=1731,20
PRIMER_LEFT_19_TM=59.296
PRIMER_RIGHT_19_TM=60.257
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=972
PRIMER_PAIR_20_PENALTY=1.020806
PRIMER_LEFT_20_PENALTY=0.766033
PRIMER_RIGHT_20_PENALTY=0.254773
PRIMER_LEFT_20_SEQUENCE=ACTTCTCCAGCCCGAAACTC
PRIMER_RIGHT_20_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_20=787,20
PRIMER_RIGHT_20=1779,20
PRIMER_LEFT_20_TM=60.766
PRIMER_RIGHT_20_TM=59.745
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=12.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=993
PRIMER_PAIR_21_PENALTY=1.022863
PRIMER_LEFT_21_PENALTY=0.766033
PRIMER_RIGHT_21_PENALTY=0.256829
PRIMER_LEFT_21_SEQUENCE=ACTTCTCCAGCCCGAAACTC
PRIMER_RIGHT_21_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_21=787,20
PRIMER_RIGHT_21=1731,20
PRIMER_LEFT_21_TM=60.766
PRIMER_RIGHT_21_TM=60.257
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=945
PRIMER_PAIR_22_PENALTY=1.041448
PRIMER_LEFT_22_PENALTY=0.289438
PRIMER_RIGHT_22_PENALTY=0.752010
PRIMER_LEFT_22_SEQUENCE=GCATCCCAATCAATCTCCAC
PRIMER_RIGHT_22_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_22=759,20
PRIMER_RIGHT_22=1725,20
PRIMER_LEFT_22_TM=60.289
PRIMER_RIGHT_22_TM=60.752
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=967
PRIMER_PAIR_23_PENALTY=1.175602
PRIMER_LEFT_23_PENALTY=0.423592
PRIMER_RIGHT_23_PENALTY=0.752010
PRIMER_LEFT_23_SEQUENCE=CAATTTGCTAATCGCATCCC
PRIMER_RIGHT_23_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_23=746,20
PRIMER_RIGHT_23=1725,20
PRIMER_LEFT_23_TM=60.424
PRIMER_RIGHT_23_TM=60.752
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.3000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=980
PRIMER_PAIR_24_PENALTY=1.199231
PRIMER_LEFT_24_PENALTY=0.942402
PRIMER_RIGHT_24_PENALTY=0.256829
PRIMER_LEFT_24_SEQUENCE=GCTAATCGCATCCCAATCAA
PRIMER_RIGHT_24_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_24=752,20
PRIMER_RIGHT_24=1731,20
PRIMER_LEFT_24_TM=60.942
PRIMER_RIGHT_24_TM=60.257
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=980
PRIMER_PAIR_25_PENALTY=1.199231
PRIMER_LEFT_25_PENALTY=0.942402
PRIMER_RIGHT_25_PENALTY=0.256829
PRIMER_LEFT_25_SEQUENCE=TTGCTAATCGCATCCCAATC
PRIMER_RIGHT_25_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_25=750,20
PRIMER_RIGHT_25=1731,20
PRIMER_LEFT_25_TM=60.942
PRIMER_RIGHT_25_TM=60.257
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.9000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=982
PRIMER_PAIR_26_PENALTY=1.268685
PRIMER_LEFT_26_PENALTY=0.516675
PRIMER_RIGHT_26_PENALTY=0.752010
PRIMER_LEFT_26_SEQUENCE=CCGAAACTCCCAATCTCATC
PRIMER_RIGHT_26_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_26=798,20
PRIMER_RIGHT_26=1725,20
PRIMER_LEFT_26_TM=59.483
PRIMER_RIGHT_26_TM=60.752
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.3000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=928
PRIMER_PAIR_27_PENALTY=1.313631
PRIMER_LEFT_27_PENALTY=1.058858
PRIMER_RIGHT_27_PENALTY=0.254773
PRIMER_LEFT_27_SEQUENCE=CGAAACTCCCAATCTCATCAA
PRIMER_RIGHT_27_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_27=799,21
PRIMER_RIGHT_27=1779,20
PRIMER_LEFT_27_TM=60.059
PRIMER_RIGHT_27_TM=59.745
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=12.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=981
PRIMER_PAIR_28_PENALTY=1.315687
PRIMER_LEFT_28_PENALTY=1.058858
PRIMER_RIGHT_28_PENALTY=0.256829
PRIMER_LEFT_28_SEQUENCE=CGAAACTCCCAATCTCATCAA
PRIMER_RIGHT_28_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_28=799,21
PRIMER_RIGHT_28=1731,20
PRIMER_LEFT_28_TM=60.059
PRIMER_RIGHT_28_TM=60.257
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=933
PRIMER_PAIR_29_PENALTY=1.324208
PRIMER_LEFT_29_PENALTY=0.572198
PRIMER_RIGHT_29_PENALTY=0.752010
PRIMER_LEFT_29_SEQUENCE=TCCAGAATCCGAAAAGTCCA
PRIMER_RIGHT_29_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_29=821,20
PRIMER_RIGHT_29=1725,20
PRIMER_LEFT_29_TM=60.572
PRIMER_RIGHT_29_TM=60.752
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=2.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=905
PRIMER_PAIR_30_PENALTY=1.343077
PRIMER_LEFT_30_PENALTY=0.044122
PRIMER_RIGHT_30_PENALTY=1.298955
PRIMER_LEFT_30_SEQUENCE=CCAGAATCCGAAAAGTCCAA
PRIMER_RIGHT_30_SEQUENCE=CGAAACCAAAAGCGACAGAA
PRIMER_LEFT_30=822,20
PRIMER_RIGHT_30=1717,20
PRIMER_LEFT_30_TM=60.044
PRIMER_RIGHT_30_TM=61.299
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=896
PRIMER_PAIR_31_PENALTY=1.361886
PRIMER_LEFT_31_PENALTY=0.609876
PRIMER_RIGHT_31_PENALTY=0.752010
PRIMER_LEFT_31_SEQUENCE=AAACTTCTCCAGCCCGAAAC
PRIMER_RIGHT_31_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_31=785,20
PRIMER_RIGHT_31=1725,20
PRIMER_LEFT_31_TM=60.610
PRIMER_RIGHT_31_TM=60.752
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=941
PRIMER_PAIR_32_PENALTY=1.375909
PRIMER_LEFT_32_PENALTY=1.121136
PRIMER_RIGHT_32_PENALTY=0.254773
PRIMER_LEFT_32_SEQUENCE=TCCCAATCTCATCAAATCCAG
PRIMER_RIGHT_32_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_32=805,21
PRIMER_RIGHT_32=1779,20
PRIMER_LEFT_32_TM=59.879
PRIMER_RIGHT_32_TM=59.745
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=12.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=975
PRIMER_PAIR_33_PENALTY=1.376295
PRIMER_LEFT_33_PENALTY=0.077340
PRIMER_RIGHT_33_PENALTY=1.298955
PRIMER_LEFT_33_SEQUENCE=GTAATACCAGATTCCGGCGA
PRIMER_RIGHT_33_SEQUENCE=CGAAACCAAAAGCGACAGAA
PRIMER_LEFT_33=849,20
PRIMER_RIGHT_33=1717,20
PRIMER_LEFT_33_TM=59.923
PRIMER_RIGHT_33_TM=61.299
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=11.4000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=869
PRIMER_PAIR_34_PENALTY=1.377966
PRIMER_LEFT_34_PENALTY=1.121136
PRIMER_RIGHT_34_PENALTY=0.256829
PRIMER_LEFT_34_SEQUENCE=TCCCAATCTCATCAAATCCAG
PRIMER_RIGHT_34_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_34=805,21
PRIMER_RIGHT_34=1731,20
PRIMER_LEFT_34_TM=59.879
PRIMER_RIGHT_34_TM=60.257
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=927
PRIMER_PAIR_35_PENALTY=1.377966
PRIMER_LEFT_35_PENALTY=1.121136
PRIMER_RIGHT_35_PENALTY=0.256829
PRIMER_LEFT_35_SEQUENCE=CTCCCAATCTCATCAAATCCA
PRIMER_RIGHT_35_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_35=804,21
PRIMER_RIGHT_35=1731,20
PRIMER_LEFT_35_TM=59.879
PRIMER_RIGHT_35_TM=60.257
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=928
PRIMER_PAIR_36_PENALTY=1.403897
PRIMER_LEFT_36_PENALTY=0.651888
PRIMER_RIGHT_36_PENALTY=0.752010
PRIMER_LEFT_36_SEQUENCE=AATCGCATCCCAATCAATCT
PRIMER_RIGHT_36_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_36=755,20
PRIMER_RIGHT_36=1725,20
PRIMER_LEFT_36_TM=59.348
PRIMER_RIGHT_36_TM=60.752
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=971
PRIMER_PAIR_37_PENALTY=1.408616
PRIMER_LEFT_37_PENALTY=1.153843
PRIMER_RIGHT_37_PENALTY=0.254773
PRIMER_LEFT_37_SEQUENCE=TCCCAATCTCATCAAATCCA
PRIMER_RIGHT_37_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_37=805,20
PRIMER_RIGHT_37=1779,20
PRIMER_LEFT_37_TM=58.846
PRIMER_RIGHT_37_TM=59.745
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.1000
PRIMER_RIGHT_37_END_STABILITY=12.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=975
PRIMER_PAIR_38_PENALTY=1.410672
PRIMER_LEFT_38_PENALTY=1.153843
PRIMER_RIGHT_38_PENALTY=0.256829
PRIMER_LEFT_38_SEQUENCE=TCCCAATCTCATCAAATCCA
PRIMER_RIGHT_38_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_38=805,20
PRIMER_RIGHT_38=1731,20
PRIMER_LEFT_38_TM=58.846
PRIMER_RIGHT_38_TM=60.257
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=927
PRIMER_PAIR_39_PENALTY=1.438213
PRIMER_LEFT_39_PENALTY=0.139259
PRIMER_RIGHT_39_PENALTY=1.298955
PRIMER_LEFT_39_SEQUENCE=ATCGCATCCCAATCAATCTC
PRIMER_RIGHT_39_SEQUENCE=CGAAACCAAAAGCGACAGAA
PRIMER_LEFT_39=756,20
PRIMER_RIGHT_39=1717,20
PRIMER_LEFT_39_TM=59.861
PRIMER_RIGHT_39_TM=61.299
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.3000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=962
PRIMER_PAIR_40_PENALTY=1.456214
PRIMER_LEFT_40_PENALTY=0.704204
PRIMER_RIGHT_40_PENALTY=0.752010
PRIMER_LEFT_40_SEQUENCE=CATCCCAATCAATCTCCACA
PRIMER_RIGHT_40_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_40=760,20
PRIMER_RIGHT_40=1725,20
PRIMER_LEFT_40_TM=59.296
PRIMER_RIGHT_40_TM=60.752
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=7.3000
PRIMER_PAIR_40_COMPL_ANY=2.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=966
PRIMER_PAIR_41_PENALTY=1.489236
PRIMER_LEFT_41_PENALTY=0.190281
PRIMER_RIGHT_41_PENALTY=1.298955
PRIMER_LEFT_41_SEQUENCE=GAAGGAAACTTCTCCAGCCC
PRIMER_RIGHT_41_SEQUENCE=CGAAACCAAAAGCGACAGAA
PRIMER_LEFT_41=780,20
PRIMER_RIGHT_41=1717,20
PRIMER_LEFT_41_TM=60.190
PRIMER_RIGHT_41_TM=61.299
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=10.9000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=938
PRIMER_PAIR_42_PENALTY=1.518043
PRIMER_LEFT_42_PENALTY=0.766033
PRIMER_RIGHT_42_PENALTY=0.752010
PRIMER_LEFT_42_SEQUENCE=ACTTCTCCAGCCCGAAACTC
PRIMER_RIGHT_42_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_42=787,20
PRIMER_RIGHT_42=1725,20
PRIMER_LEFT_42_TM=60.766
PRIMER_RIGHT_42_TM=60.752
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.3000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=939
PRIMER_PAIR_43_PENALTY=1.575727
PRIMER_LEFT_43_PENALTY=1.320954
PRIMER_RIGHT_43_PENALTY=0.254773
PRIMER_LEFT_43_SEQUENCE=AATACCAGATTCCGGCGAAG
PRIMER_RIGHT_43_SEQUENCE=ATAATGATCTCAATGGGCGG
PRIMER_LEFT_43=851,20
PRIMER_RIGHT_43=1779,20
PRIMER_LEFT_43_TM=61.321
PRIMER_RIGHT_43_TM=59.745
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.7000
PRIMER_RIGHT_43_END_STABILITY=12.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=929
PRIMER_PAIR_44_PENALTY=1.577783
PRIMER_LEFT_44_PENALTY=1.320954
PRIMER_RIGHT_44_PENALTY=0.256829
PRIMER_LEFT_44_SEQUENCE=AATACCAGATTCCGGCGAAG
PRIMER_RIGHT_44_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_44=851,20
PRIMER_RIGHT_44=1731,20
PRIMER_LEFT_44_TM=61.321
PRIMER_RIGHT_44_TM=60.257
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.7000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=881
PRIMER_PAIR_45_PENALTY=1.581053
PRIMER_LEFT_45_PENALTY=1.324224
PRIMER_RIGHT_45_PENALTY=0.256829
PRIMER_LEFT_45_SEQUENCE=CATCCCAATCAATCTCCACAG
PRIMER_RIGHT_45_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_45=760,21
PRIMER_RIGHT_45=1731,20
PRIMER_LEFT_45_TM=60.324
PRIMER_RIGHT_45_TM=60.257
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=972
PRIMER_PAIR_46_PENALTY=1.588392
PRIMER_LEFT_46_PENALTY=0.289438
PRIMER_RIGHT_46_PENALTY=1.298955
PRIMER_LEFT_46_SEQUENCE=GCATCCCAATCAATCTCCAC
PRIMER_RIGHT_46_SEQUENCE=CGAAACCAAAAGCGACAGAA
PRIMER_LEFT_46=759,20
PRIMER_RIGHT_46=1717,20
PRIMER_LEFT_46_TM=60.289
PRIMER_RIGHT_46_TM=61.299
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=959
PRIMER_PAIR_47_PENALTY=1.624545
PRIMER_LEFT_47_PENALTY=1.367716
PRIMER_RIGHT_47_PENALTY=0.256829
PRIMER_LEFT_47_SEQUENCE=CAGAAGGAAACTTCTCCAGCC
PRIMER_RIGHT_47_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_47=778,21
PRIMER_RIGHT_47=1731,20
PRIMER_LEFT_47_TM=60.368
PRIMER_RIGHT_47_TM=60.257
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=954
PRIMER_PAIR_48_PENALTY=1.691027
PRIMER_LEFT_48_PENALTY=1.434197
PRIMER_RIGHT_48_PENALTY=0.256829
PRIMER_LEFT_48_SEQUENCE=GGACAATTTGCTAATCGCATC
PRIMER_RIGHT_48_SEQUENCE=AACTTGGCATCAAGCGAAAC
PRIMER_LEFT_48=743,21
PRIMER_RIGHT_48=1731,20
PRIMER_LEFT_48_TM=59.566
PRIMER_RIGHT_48_TM=60.257
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.1000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=989
PRIMER_PAIR_49_PENALTY=1.694412
PRIMER_LEFT_49_PENALTY=0.942402
PRIMER_RIGHT_49_PENALTY=0.752010
PRIMER_LEFT_49_SEQUENCE=GCTAATCGCATCCCAATCAA
PRIMER_RIGHT_49_SEQUENCE=GCATCAAGCGAAACCAAAAG
PRIMER_LEFT_49=752,20
PRIMER_RIGHT_49=1725,20
PRIMER_LEFT_49_TM=60.942
PRIMER_RIGHT_49_TM=60.752
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=974
=
SEQUENCE_ID=MAGIC.175_4_7371147_7371267_wu-0_can-0_1280_409585_TRUE
SEQUENCE_TARGET=950,220
SEQUENCE_TEMPLATE=AAAANNANCNAAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNCGTGCATCATTGAATCTGTACTTTATGATGAGTCACGAAGACATAAGTCTCAGATCTGACTAAGAATCTTTTTTCGAGTTTCCCTGAAATGTATCCATTGAAACTGTCTACTNNNTTTTTTTTCGTGTCCANTATACAAANTTTTCTCTATCGTGTGCTTTCAAANATTCGTTAATATGTNTTTTTGCTTATNTNGAGNCANTATACATNNTTTTTTTGTATAAAATGATATTAGTANTATTCCTAACTAAAGAATAAACAATAATGTCAAGAATTGCATCATTATGTAGTATTTTACTATGNNATAATNNNANNATTGTTTGAACCATGCATGATTTGACACTTNTTATAATTCCATTCAAANAAGCAAAATTAAACAATCTCCGGAAGTTGAAAATGTGTGGTGTTAATTNAACTAAATATACAATGAATCTANNTTTTTNATTGTGTTTTNTATNATTTTAAATNGTACNNANAATAAANTAATNTATAANGAATTATATACCANAAGAAAGACGGTGCNTACCAAACTCATTAGTGATTNTATTATTGGTGGGTTTTAGNNNNANNNNNNNTTAATAGTCTTATNAAAAAANNNNNNNNNNNNNNNNNNNNNNGTATACGACACACGAGGCAAGAAAANTATGTATCTAAAATGTAATGTTAATTTTNTGTAACACNATTTTAATATAAACTTTGACATCGCTTTTTTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNGTGATACNNNNAAANANANTNNNNTNATNNNNNNNNNNNNTNCNNAGNNNTNCAAANNCANNTGGTTNANNNNAGNCATTNNNACNGNTNNNCNANNAAANNTNAANCGNNNNTNAANAGNAGACNTNTNANNNNATNCNNACANANATCNAACCNNNNTTTAANANNNNNNNNNNNNNNNNNANANNACAGNATCAAACCNATTCTTTAACANAATCAACTGAANCAAACATAAACCAGACCGAACTAAAATGGCTTTGATTTGGGTTTAATAATAGTNCCATNCACCACTNAAACGAACTAACCAAACAAAAAACAAAACCAAACGAAAACTGAACAGAAAGGANTNNNNNNNNNNNATTANTTTTTACNAGGATCGTATTAACCAAACCGATTAAACAAACATCTTAACNATTTANCTACAGTACTTNCCATTATNTTANTGTATCAAATTCAACTTNTANATAATATGTAAAATATAATACACAGCTAAATTGAACTCGAACTNGTCAAAGCTAAACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTACTTCTATTCTATATTAGCTAGACATCTTANTTTATNNTGTTAACCTGNTTANAANTTTNNAAAATGATTTGTTTAAAACAACTAAATTAATACATTTGGTGTGNCGTGCATCCGAATNGCATGTCNAATNTNATATGGAATTAGTGTCAATAACAAATAAAACAATGAANTTTACTGAAATGATAACGTGATTTGNTTAAACAGTCATAATGTATAACGTGATATAGGTAGTANATGTATGTAGACTGGGTNANGTTATGTGATTCTTCTCNGCTCGATAATCAAGGAGGAATCTTATAACATGATCCAGTC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_6004202_6004322_ler-0_kn-0_121639_69044_TRUE
SEQUENCE_TARGET=950,220
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAAAAAACNAACTAACANAAAAAANTTGTCNACCTAAAATTNNNNATATATATANANACGTGCATGTGTAAGATTANATTTANATTTTCTATTTTCTCNNNNTCATGGGCAACAATAAATTATCACCTCCCAACNTTAGTAATGACATTAACTCTCTCCACATCGATTATGAAAATGCTCTCAACCATNTCGTTACAACAACTGTTTAATACATGGACAAACTNCATGACTATCAAAATTACAACTTCTCANGCCCCATTAAACAAGCCATTTANNNNNNNNNTGTCAATGCAAAACAGATAATAAAAANTTGCATTTTCTAAAGAAATGATATTGCATTTGTGAAAGAGAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_6859870_6860178_po-0_ler-0_94995_2403_TRUE
SEQUENCE_TARGET=950,408
SEQUENCE_TEMPLATE=TCCCAAATTTTCTCAGAGGAGCTTCAGAATCTGCCGTGACAACAGCTATATCAGCTCTCAGCTTTTCACCTGATGGAGAGGTAAAATTTATGCATNAACACATTGTAGCTTCTGCACCAAACATTAAAGTNGACAAGTTCCCGATGAATACTCNTGATGAGATCCTAAGAATAAAATCTCTTTGTATGGTAGGGATTGGTTGCGTTTTCAGAAAACGGTTTGATGATCAGATGGTGGTCACTAGGATCGGTTTGGTGGGAGAAACTAAGCCAAAGTCTCACACNTATTCANTGCACCAAACTAATATTTATCCATCCATGGGATGGATTTTCATCAAGCTCTTCACGAACAAGTGTGATCTCAAGTATCTCTAACGACGAACAAGAACTTCCTCTTCAGGTACTTAATTGTTTATCATNATCTNATTGTTTTGGCTTTTAAAACCGATACTTATTCTACTCNGTGTGATCGGAACAGGAAACCGCAAAGAACATAAGTCACGTAGAGCGGCTAAAGCAACTTGTTCAACATCTTGATCTCTCTTATCGCCTCGAATGGGCCAGTGAACGCAAAGTAGTTTTAACAAGACACAATGTCGAGTTGGGCACTTGCCTGTTGTAACAGTCAAGGTAAGAATNCAGACGATATTCTNAATCCTTGNTCCTCATTCTGCTCGAGTTTTGTTTTAGAGGACTCATATCGAGTCCATGTTNTAGGTTCTCNGGAGGCTATCNAACGTTCCTTTTTTTGTGTNNTANTTGAGAACGAAATTGACTGTTCTTGTTTTGTATATGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGCCTCGACTCTAAGCGCTGCCAAACTGATTTATGGTTTTTATCCTTCTCGTAGGAATCCCTAGAGTGATAGGATGCTCGATGCTCATAGTGTTTGTCACGGGATTGGACTTTCTTAGCATAAGCTTGGTTTCTGGAGTTAACATTCCCATGAGAACTTTCATAGGAGAAGCTCTCTGATCTTTTCCTATCCCTTGGCGAGTAGTTTCCTCTCTTCCTTTCATTATGTTTGTCATCCAATGCTTTATTATACCTATCAGCCGAAACATCGGATGAGAGAGTTCNNTNTNNCNTNTNGAATTCANTCAATATNCCNNTAAATANGTGCCAACAAGATCTTAGANNATGNGTNTGATTGGTTNTCCTAGTTNNCNNNNAAAAAANNTGAAAAANAATTNTGCNCAATTNATCTNTTTTTACANCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAAAAACACNANAACAGTGTAAATTGTTNNACTNTANATTTATTTTAGTGGCTGAAACANNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_14527619_14528325_wil-2_sf-2_43048_10864_TRUE
SEQUENCE_TARGET=950,806
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_8809121_8809158_oy-0_can-0_7830_51195_TRUE
SEQUENCE_TARGET=950,137
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTAANGATTTGCACCCGAACATATCTAGTCCTCATAATCAATTNCTCCNAGNTTTATTAGTANATTACCGTGTCTCAANTTTTGCNNTTTTNNNNCNNNAAAAAAATAGACATGGACCAGTTTTTAGGACAAGCTTATTTGGAGGAAAAGTTATNATTTCGACNGATATTGGNTTNAACATGGAGATAGCAAAGACAAATCATATTCCTGGTATGCCGAAGAGCTTAGCGCGGCTATTTGGGNCAAACAACTTGTTNGTGAATAAGGATACTCATAAACATGCCCGCAGCTTGACAAACCAGTTTTTAGGTTCGCAAGCTTTGAAATTAAGNATGNTTCAAGACATCGATTTCTTGGNTCGCACANANCTGAAGGAAGGAGCTAGGAAAGGCAGCNTTGACATCAAGGAAACGACAAGCAAGGTNAAAACAAAAAGTCATCCATTTTGANANTTTTTGTAGCTCATAACAAGANAACTTTCCACTTAANAAATCATCTTATCAANTTCAAACNTTACAGATCNTNATTGAATGTCTTGCGAAGAAAGTAATGGGGGAGATGGAACCAGANGCAGCAAAAGAACTCACACTCTGTTGGACATTTTTCCCAAGGGAATGGTTTGGATTCGCTTGGAATATCCCNGGGACTGGTGTCTANCGAATGGTGAAGGTGCATATTTTTNNATCATCTACCTTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCGNTTGTGTNTTCATNTTTTGTCGCAAATAGCNNGGGCCNATAACCNTTTTTNNNNNNNNNTATNACACAAATANNNNNNNNNNNNNNNNNNNNTNCATTTTGTCTTGAACTGAGGAAGTGTATTTTGTGANAGGCAAGAAANCGGATGATGAAGNTANTAAAGGAGACGGTACTTAAGAAAAGAGCATCAGGAGAGGAATTGGGGGACTTTTTCAAGACNATCTTTGGAGATACTGAAAGAGGCGTGNAAACTATAAGNTTAGAGAGTGCNACNGAGTACATATTTACTCTGTTTTTGCTTGCTAACGAAACAACACCAGCGGTNCTTGCGGCTACAATAAAGCTCATAAGTGACCATCCTAAAGTNATGCAAGAGTTACAGAGAGAGCATGAAGGAATTGTCCGAGNTAAGATTGAGAAAAATGAGAAAGCTGACCTAACNTGGGAAGACTACAAATCAATGACTTTTACCCAGATGGTGAGTATCCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACAAGNACGGTACCNACGGTGCTTAGAATAATTNATCATGAGTTNCAATNTGGTGANTATACAATTCCAGCAGGTTGNATCTTTATGGNTTATCCTTANGTGNNNNNNNNNNNNNNNNNGNATGATGACNCTTTANCNTTTAATCCATGGCGCTNGAAGGTAATTTANATTTCATCTTNATCTTTTAATTATTCTTNNNNNNNNNNNNNNNNNNNNNNGGAATTTAAANNNNCATATATTAAACNNAATTATCAGGGAAAAGACTTGAGCGCGATNGTCTCCAGGACTTACATTCCNTTTGGNTNNGGTTCTAGACTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.670347
PRIMER_LEFT_0_PENALTY=0.067187
PRIMER_RIGHT_0_PENALTY=0.603160
PRIMER_LEFT_0_SEQUENCE=TTGCGAAGAAAGTAATGGGG
PRIMER_RIGHT_0_SEQUENCE=CGGACAATTCCTTCATGCTC
PRIMER_LEFT_0=718,20
PRIMER_RIGHT_0=1396,20
PRIMER_LEFT_0_TM=60.067
PRIMER_RIGHT_0_TM=60.603
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=11.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=679
PRIMER_PAIR_1_PENALTY=0.670347
PRIMER_LEFT_1_PENALTY=0.067187
PRIMER_RIGHT_1_PENALTY=0.603160
PRIMER_LEFT_1_SEQUENCE=TTGCGAAGAAAGTAATGGGG
PRIMER_RIGHT_1_SEQUENCE=TCGGACAATTCCTTCATGCT
PRIMER_LEFT_1=718,20
PRIMER_RIGHT_1=1397,20
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=60.603
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=680
PRIMER_PAIR_2_PENALTY=0.681135
PRIMER_LEFT_2_PENALTY=0.067187
PRIMER_RIGHT_2_PENALTY=0.613948
PRIMER_LEFT_2_SEQUENCE=TTGCGAAGAAAGTAATGGGG
PRIMER_RIGHT_2_SEQUENCE=CGCTGGTGTTGTTTCGTTAG
PRIMER_LEFT_2=718,20
PRIMER_RIGHT_2=1313,20
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.386
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=596
PRIMER_PAIR_3_PENALTY=0.762909
PRIMER_LEFT_3_PENALTY=0.159750
PRIMER_RIGHT_3_PENALTY=0.603160
PRIMER_LEFT_3_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_3_SEQUENCE=CGGACAATTCCTTCATGCTC
PRIMER_LEFT_3=583,20
PRIMER_RIGHT_3=1396,20
PRIMER_LEFT_3_TM=59.840
PRIMER_RIGHT_3_TM=60.603
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=814
PRIMER_PAIR_4_PENALTY=0.786593
PRIMER_LEFT_4_PENALTY=0.159750
PRIMER_RIGHT_4_PENALTY=0.626843
PRIMER_LEFT_4_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_4_SEQUENCE=AAAGTCCCCCAATTCCTCTC
PRIMER_LEFT_4=583,20
PRIMER_RIGHT_4=1202,20
PRIMER_LEFT_4_TM=59.840
PRIMER_RIGHT_4_TM=59.373
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=620
PRIMER_PAIR_5_PENALTY=0.898397
PRIMER_LEFT_5_PENALTY=0.159750
PRIMER_RIGHT_5_PENALTY=0.738647
PRIMER_LEFT_5_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_5_SEQUENCE=AAAAAGTCCCCCAATTCCTC
PRIMER_LEFT_5=583,20
PRIMER_RIGHT_5=1204,20
PRIMER_LEFT_5_TM=59.840
PRIMER_RIGHT_5_TM=59.261
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=622
PRIMER_PAIR_6_PENALTY=0.898397
PRIMER_LEFT_6_PENALTY=0.159750
PRIMER_RIGHT_6_PENALTY=0.738647
PRIMER_LEFT_6_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_6_SEQUENCE=GAAAAAGTCCCCCAATTCCT
PRIMER_LEFT_6=583,20
PRIMER_RIGHT_6=1205,20
PRIMER_LEFT_6_TM=59.840
PRIMER_RIGHT_6_TM=59.261
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=623
PRIMER_PAIR_7_PENALTY=1.051010
PRIMER_LEFT_7_PENALTY=0.447850
PRIMER_RIGHT_7_PENALTY=0.603160
PRIMER_LEFT_7_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_7_SEQUENCE=CGGACAATTCCTTCATGCTC
PRIMER_LEFT_7=795,20
PRIMER_RIGHT_7=1396,20
PRIMER_LEFT_7_TM=60.448
PRIMER_RIGHT_7_TM=60.603
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=602
PRIMER_PAIR_8_PENALTY=1.051010
PRIMER_LEFT_8_PENALTY=0.447850
PRIMER_RIGHT_8_PENALTY=0.603160
PRIMER_LEFT_8_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_8_SEQUENCE=TCGGACAATTCCTTCATGCT
PRIMER_LEFT_8=795,20
PRIMER_RIGHT_8=1397,20
PRIMER_LEFT_8_TM=60.448
PRIMER_RIGHT_8_TM=60.603
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=603
PRIMER_PAIR_9_PENALTY=1.061798
PRIMER_LEFT_9_PENALTY=0.447850
PRIMER_RIGHT_9_PENALTY=0.613948
PRIMER_LEFT_9_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_9_SEQUENCE=CGCTGGTGTTGTTTCGTTAG
PRIMER_LEFT_9=795,20
PRIMER_RIGHT_9=1313,20
PRIMER_LEFT_9_TM=60.448
PRIMER_RIGHT_9_TM=59.386
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=5.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=519
PRIMER_PAIR_10_PENALTY=1.074694
PRIMER_LEFT_10_PENALTY=0.447850
PRIMER_RIGHT_10_PENALTY=0.626843
PRIMER_LEFT_10_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_10_SEQUENCE=AAAGTCCCCCAATTCCTCTC
PRIMER_LEFT_10=795,20
PRIMER_RIGHT_10=1202,20
PRIMER_LEFT_10_TM=60.448
PRIMER_RIGHT_10_TM=59.373
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=408
PRIMER_PAIR_11_PENALTY=1.156726
PRIMER_LEFT_11_PENALTY=0.159750
PRIMER_RIGHT_11_PENALTY=0.996977
PRIMER_LEFT_11_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_11_SEQUENCE=GTCTTGAAAAAGTCCCCCAA
PRIMER_LEFT_11=583,20
PRIMER_RIGHT_11=1210,20
PRIMER_LEFT_11_TM=59.840
PRIMER_RIGHT_11_TM=59.003
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=10.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=628
PRIMER_PAIR_12_PENALTY=1.182632
PRIMER_LEFT_12_PENALTY=0.159750
PRIMER_RIGHT_12_PENALTY=1.022883
PRIMER_LEFT_12_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_12_SEQUENCE=TGAAAAAGTCCCCCAATTCC
PRIMER_LEFT_12=583,20
PRIMER_RIGHT_12=1206,20
PRIMER_LEFT_12_TM=59.840
PRIMER_RIGHT_12_TM=61.023
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=624
PRIMER_PAIR_13_PENALTY=1.186498
PRIMER_LEFT_13_PENALTY=0.447850
PRIMER_RIGHT_13_PENALTY=0.738647
PRIMER_LEFT_13_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_13_SEQUENCE=AAAAAGTCCCCCAATTCCTC
PRIMER_LEFT_13=795,20
PRIMER_RIGHT_13=1204,20
PRIMER_LEFT_13_TM=60.448
PRIMER_RIGHT_13_TM=59.261
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=410
PRIMER_PAIR_14_PENALTY=1.186498
PRIMER_LEFT_14_PENALTY=0.447850
PRIMER_RIGHT_14_PENALTY=0.738647
PRIMER_LEFT_14_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_14_SEQUENCE=GAAAAAGTCCCCCAATTCCT
PRIMER_LEFT_14=795,20
PRIMER_RIGHT_14=1205,20
PRIMER_LEFT_14_TM=60.448
PRIMER_RIGHT_14_TM=59.261
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=411
PRIMER_PAIR_15_PENALTY=1.209323
PRIMER_LEFT_15_PENALTY=0.447850
PRIMER_RIGHT_15_PENALTY=0.761472
PRIMER_LEFT_15_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_15_SEQUENCE=CCAATTCCTCTCCTGATGCT
PRIMER_LEFT_15=795,20
PRIMER_RIGHT_15=1194,20
PRIMER_LEFT_15_TM=60.448
PRIMER_RIGHT_15_TM=59.239
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=400
PRIMER_PAIR_16_PENALTY=1.225982
PRIMER_LEFT_16_PENALTY=0.622823
PRIMER_RIGHT_16_PENALTY=0.603160
PRIMER_LEFT_16_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_16_SEQUENCE=CGGACAATTCCTTCATGCTC
PRIMER_LEFT_16=402,20
PRIMER_RIGHT_16=1396,20
PRIMER_LEFT_16_TM=60.623
PRIMER_RIGHT_16_TM=60.603
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.2000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=995
PRIMER_PAIR_17_PENALTY=1.225982
PRIMER_LEFT_17_PENALTY=0.622823
PRIMER_RIGHT_17_PENALTY=0.603160
PRIMER_LEFT_17_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_17_SEQUENCE=TCGGACAATTCCTTCATGCT
PRIMER_LEFT_17=402,20
PRIMER_RIGHT_17=1397,20
PRIMER_LEFT_17_TM=60.623
PRIMER_RIGHT_17_TM=60.603
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.2000
PRIMER_RIGHT_17_END_STABILITY=8.1000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=996
PRIMER_PAIR_18_PENALTY=1.236770
PRIMER_LEFT_18_PENALTY=0.622823
PRIMER_RIGHT_18_PENALTY=0.613948
PRIMER_LEFT_18_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_18_SEQUENCE=CGCTGGTGTTGTTTCGTTAG
PRIMER_LEFT_18=402,20
PRIMER_RIGHT_18=1313,20
PRIMER_LEFT_18_TM=60.623
PRIMER_RIGHT_18_TM=59.386
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.2000
PRIMER_RIGHT_18_END_STABILITY=5.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=912
PRIMER_PAIR_19_PENALTY=1.249666
PRIMER_LEFT_19_PENALTY=0.622823
PRIMER_RIGHT_19_PENALTY=0.626843
PRIMER_LEFT_19_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_19_SEQUENCE=AAAGTCCCCCAATTCCTCTC
PRIMER_LEFT_19=402,20
PRIMER_RIGHT_19=1202,20
PRIMER_LEFT_19_TM=60.623
PRIMER_RIGHT_19_TM=59.373
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.2000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=801
PRIMER_PAIR_20_PENALTY=1.264235
PRIMER_LEFT_20_PENALTY=0.159750
PRIMER_RIGHT_20_PENALTY=1.104485
PRIMER_LEFT_20_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_20_SEQUENCE=AAAAGTCCCCCAATTCCTCT
PRIMER_LEFT_20=583,20
PRIMER_RIGHT_20=1203,20
PRIMER_LEFT_20_TM=59.840
PRIMER_RIGHT_20_TM=58.896
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=621
PRIMER_PAIR_21_PENALTY=1.328924
PRIMER_LEFT_21_PENALTY=0.159750
PRIMER_RIGHT_21_PENALTY=1.169174
PRIMER_LEFT_21_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_21_SEQUENCE=AAAAAGTCCCCCAATTCCTCT
PRIMER_LEFT_21=583,20
PRIMER_RIGHT_21=1204,21
PRIMER_LEFT_21_TM=59.840
PRIMER_RIGHT_21_TM=60.169
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=622
PRIMER_PAIR_22_PENALTY=1.355171
PRIMER_LEFT_22_PENALTY=0.159750
PRIMER_RIGHT_22_PENALTY=1.195421
PRIMER_LEFT_22_SEQUENCE=CATCAAGGAAACGACAAGCA
PRIMER_RIGHT_22_SEQUENCE=AGTCCCCCAATTCCTCTCCT
PRIMER_LEFT_22=583,20
PRIMER_RIGHT_22=1200,20
PRIMER_LEFT_22_TM=59.840
PRIMER_RIGHT_22_TM=61.195
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=618
PRIMER_PAIR_23_PENALTY=1.361470
PRIMER_LEFT_23_PENALTY=0.622823
PRIMER_RIGHT_23_PENALTY=0.738647
PRIMER_LEFT_23_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_23_SEQUENCE=AAAAAGTCCCCCAATTCCTC
PRIMER_LEFT_23=402,20
PRIMER_RIGHT_23=1204,20
PRIMER_LEFT_23_TM=60.623
PRIMER_RIGHT_23_TM=59.261
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.2000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=803
PRIMER_PAIR_24_PENALTY=1.361470
PRIMER_LEFT_24_PENALTY=0.622823
PRIMER_RIGHT_24_PENALTY=0.738647
PRIMER_LEFT_24_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_24_SEQUENCE=GAAAAAGTCCCCCAATTCCT
PRIMER_LEFT_24=402,20
PRIMER_RIGHT_24=1205,20
PRIMER_LEFT_24_TM=60.623
PRIMER_RIGHT_24_TM=59.261
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.2000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=804
PRIMER_PAIR_25_PENALTY=1.384295
PRIMER_LEFT_25_PENALTY=0.622823
PRIMER_RIGHT_25_PENALTY=0.761472
PRIMER_LEFT_25_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_25_SEQUENCE=CCAATTCCTCTCCTGATGCT
PRIMER_LEFT_25=402,20
PRIMER_RIGHT_25=1194,20
PRIMER_LEFT_25_TM=60.623
PRIMER_RIGHT_25_TM=59.239
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.2000
PRIMER_RIGHT_25_END_STABILITY=8.1000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=793
PRIMER_PAIR_26_PENALTY=1.399621
PRIMER_LEFT_26_PENALTY=0.796461
PRIMER_RIGHT_26_PENALTY=0.603160
PRIMER_LEFT_26_SEQUENCE=CAAGGAAACGACAAGCAAGG
PRIMER_RIGHT_26_SEQUENCE=TCGGACAATTCCTTCATGCT
PRIMER_LEFT_26=586,20
PRIMER_RIGHT_26=1397,20
PRIMER_LEFT_26_TM=60.796
PRIMER_RIGHT_26_TM=60.603
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=812
PRIMER_PAIR_27_PENALTY=1.423304
PRIMER_LEFT_27_PENALTY=0.796461
PRIMER_RIGHT_27_PENALTY=0.626843
PRIMER_LEFT_27_SEQUENCE=CAAGGAAACGACAAGCAAGG
PRIMER_RIGHT_27_SEQUENCE=AAAGTCCCCCAATTCCTCTC
PRIMER_LEFT_27=586,20
PRIMER_RIGHT_27=1202,20
PRIMER_LEFT_27_TM=60.796
PRIMER_RIGHT_27_TM=59.373
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=617
PRIMER_PAIR_28_PENALTY=1.444827
PRIMER_LEFT_28_PENALTY=0.447850
PRIMER_RIGHT_28_PENALTY=0.996977
PRIMER_LEFT_28_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_28_SEQUENCE=GTCTTGAAAAAGTCCCCCAA
PRIMER_LEFT_28=795,20
PRIMER_RIGHT_28=1210,20
PRIMER_LEFT_28_TM=60.448
PRIMER_RIGHT_28_TM=59.003
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=416
PRIMER_PAIR_29_PENALTY=1.450064
PRIMER_LEFT_29_PENALTY=0.836116
PRIMER_RIGHT_29_PENALTY=0.613948
PRIMER_LEFT_29_SEQUENCE=GGAAGGAGCTAGGAAAGGCA
PRIMER_RIGHT_29_SEQUENCE=CGCTGGTGTTGTTTCGTTAG
PRIMER_LEFT_29=556,20
PRIMER_RIGHT_29=1313,20
PRIMER_LEFT_29_TM=60.836
PRIMER_RIGHT_29_TM=59.386
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=5.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=758
PRIMER_PAIR_30_PENALTY=1.470733
PRIMER_LEFT_30_PENALTY=0.447850
PRIMER_RIGHT_30_PENALTY=1.022883
PRIMER_LEFT_30_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_30_SEQUENCE=TGAAAAAGTCCCCCAATTCC
PRIMER_LEFT_30=795,20
PRIMER_RIGHT_30=1206,20
PRIMER_LEFT_30_TM=60.448
PRIMER_RIGHT_30_TM=61.023
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=412
PRIMER_PAIR_31_PENALTY=1.513186
PRIMER_LEFT_31_PENALTY=0.910027
PRIMER_RIGHT_31_PENALTY=0.603160
PRIMER_LEFT_31_SEQUENCE=CCAAGGGAATGGTTTGGATT
PRIMER_RIGHT_31_SEQUENCE=CGGACAATTCCTTCATGCTC
PRIMER_LEFT_31=789,20
PRIMER_RIGHT_31=1396,20
PRIMER_LEFT_31_TM=60.910
PRIMER_RIGHT_31_TM=60.603
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=608
PRIMER_PAIR_32_PENALTY=1.513186
PRIMER_LEFT_32_PENALTY=0.910027
PRIMER_RIGHT_32_PENALTY=0.603160
PRIMER_LEFT_32_SEQUENCE=CCAAGGGAATGGTTTGGATT
PRIMER_RIGHT_32_SEQUENCE=TCGGACAATTCCTTCATGCT
PRIMER_LEFT_32=789,20
PRIMER_RIGHT_32=1397,20
PRIMER_LEFT_32_TM=60.910
PRIMER_RIGHT_32_TM=60.603
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=609
PRIMER_PAIR_33_PENALTY=1.523974
PRIMER_LEFT_33_PENALTY=0.910027
PRIMER_RIGHT_33_PENALTY=0.613948
PRIMER_LEFT_33_SEQUENCE=CCAAGGGAATGGTTTGGATT
PRIMER_RIGHT_33_SEQUENCE=CGCTGGTGTTGTTTCGTTAG
PRIMER_LEFT_33=789,20
PRIMER_RIGHT_33=1313,20
PRIMER_LEFT_33_TM=60.910
PRIMER_RIGHT_33_TM=59.386
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=525
PRIMER_PAIR_34_PENALTY=1.535108
PRIMER_LEFT_34_PENALTY=0.796461
PRIMER_RIGHT_34_PENALTY=0.738647
PRIMER_LEFT_34_SEQUENCE=CAAGGAAACGACAAGCAAGG
PRIMER_RIGHT_34_SEQUENCE=AAAAAGTCCCCCAATTCCTC
PRIMER_LEFT_34=586,20
PRIMER_RIGHT_34=1204,20
PRIMER_LEFT_34_TM=60.796
PRIMER_RIGHT_34_TM=59.261
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=619
PRIMER_PAIR_35_PENALTY=1.535108
PRIMER_LEFT_35_PENALTY=0.796461
PRIMER_RIGHT_35_PENALTY=0.738647
PRIMER_LEFT_35_SEQUENCE=CAAGGAAACGACAAGCAAGG
PRIMER_RIGHT_35_SEQUENCE=GAAAAAGTCCCCCAATTCCT
PRIMER_LEFT_35=586,20
PRIMER_RIGHT_35=1205,20
PRIMER_LEFT_35_TM=60.796
PRIMER_RIGHT_35_TM=59.261
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=620
PRIMER_PAIR_36_PENALTY=1.536870
PRIMER_LEFT_36_PENALTY=0.910027
PRIMER_RIGHT_36_PENALTY=0.626843
PRIMER_LEFT_36_SEQUENCE=CCAAGGGAATGGTTTGGATT
PRIMER_RIGHT_36_SEQUENCE=AAAGTCCCCCAATTCCTCTC
PRIMER_LEFT_36=789,20
PRIMER_RIGHT_36=1202,20
PRIMER_LEFT_36_TM=60.910
PRIMER_RIGHT_36_TM=59.373
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=414
PRIMER_PAIR_37_PENALTY=1.552336
PRIMER_LEFT_37_PENALTY=0.447850
PRIMER_RIGHT_37_PENALTY=1.104485
PRIMER_LEFT_37_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_37_SEQUENCE=AAAAGTCCCCCAATTCCTCT
PRIMER_LEFT_37=795,20
PRIMER_RIGHT_37=1203,20
PRIMER_LEFT_37_TM=60.448
PRIMER_RIGHT_37_TM=58.896
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=409
PRIMER_PAIR_38_PENALTY=1.556526
PRIMER_LEFT_38_PENALTY=0.953367
PRIMER_RIGHT_38_PENALTY=0.603160
PRIMER_LEFT_38_SEQUENCE=TCAAGGAAACGACAAGCAAG
PRIMER_RIGHT_38_SEQUENCE=CGGACAATTCCTTCATGCTC
PRIMER_LEFT_38=585,20
PRIMER_RIGHT_38=1396,20
PRIMER_LEFT_38_TM=59.047
PRIMER_RIGHT_38_TM=60.603
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=812
PRIMER_PAIR_39_PENALTY=1.557933
PRIMER_LEFT_39_PENALTY=0.796461
PRIMER_RIGHT_39_PENALTY=0.761472
PRIMER_LEFT_39_SEQUENCE=CAAGGAAACGACAAGCAAGG
PRIMER_RIGHT_39_SEQUENCE=CCAATTCCTCTCCTGATGCT
PRIMER_LEFT_39=586,20
PRIMER_RIGHT_39=1194,20
PRIMER_LEFT_39_TM=60.796
PRIMER_RIGHT_39_TM=59.239
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=609
PRIMER_PAIR_40_PENALTY=1.574764
PRIMER_LEFT_40_PENALTY=0.836116
PRIMER_RIGHT_40_PENALTY=0.738647
PRIMER_LEFT_40_SEQUENCE=GGAAGGAGCTAGGAAAGGCA
PRIMER_RIGHT_40_SEQUENCE=AAAAAGTCCCCCAATTCCTC
PRIMER_LEFT_40=556,20
PRIMER_RIGHT_40=1204,20
PRIMER_LEFT_40_TM=60.836
PRIMER_RIGHT_40_TM=59.261
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=649
PRIMER_PAIR_41_PENALTY=1.574764
PRIMER_LEFT_41_PENALTY=0.836116
PRIMER_RIGHT_41_PENALTY=0.738647
PRIMER_LEFT_41_SEQUENCE=GGAAGGAGCTAGGAAAGGCA
PRIMER_RIGHT_41_SEQUENCE=GAAAAAGTCCCCCAATTCCT
PRIMER_LEFT_41=556,20
PRIMER_RIGHT_41=1205,20
PRIMER_LEFT_41_TM=60.836
PRIMER_RIGHT_41_TM=59.261
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=650
PRIMER_PAIR_42_PENALTY=1.580210
PRIMER_LEFT_42_PENALTY=0.953367
PRIMER_RIGHT_42_PENALTY=0.626843
PRIMER_LEFT_42_SEQUENCE=TCAAGGAAACGACAAGCAAG
PRIMER_RIGHT_42_SEQUENCE=AAAGTCCCCCAATTCCTCTC
PRIMER_LEFT_42=585,20
PRIMER_RIGHT_42=1202,20
PRIMER_LEFT_42_TM=59.047
PRIMER_RIGHT_42_TM=59.373
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=618
PRIMER_PAIR_43_PENALTY=1.617024
PRIMER_LEFT_43_PENALTY=0.447850
PRIMER_RIGHT_43_PENALTY=1.169174
PRIMER_LEFT_43_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_43_SEQUENCE=AAAAAGTCCCCCAATTCCTCT
PRIMER_LEFT_43=795,20
PRIMER_RIGHT_43=1204,21
PRIMER_LEFT_43_TM=60.448
PRIMER_RIGHT_43_TM=60.169
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=410
PRIMER_PAIR_44_PENALTY=1.643272
PRIMER_LEFT_44_PENALTY=0.447850
PRIMER_RIGHT_44_PENALTY=1.195421
PRIMER_LEFT_44_SEQUENCE=GAATGGTTTGGATTCGCTTG
PRIMER_RIGHT_44_SEQUENCE=AGTCCCCCAATTCCTCTCCT
PRIMER_LEFT_44=795,20
PRIMER_RIGHT_44=1200,20
PRIMER_LEFT_44_TM=60.448
PRIMER_RIGHT_44_TM=61.195
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=406
PRIMER_PAIR_45_PENALTY=1.645705
PRIMER_LEFT_45_PENALTY=0.622823
PRIMER_RIGHT_45_PENALTY=1.022883
PRIMER_LEFT_45_SEQUENCE=AGAGCTTAGCGCGGCTATTT
PRIMER_RIGHT_45_SEQUENCE=TGAAAAAGTCCCCCAATTCC
PRIMER_LEFT_45=402,20
PRIMER_RIGHT_45=1206,20
PRIMER_LEFT_45_TM=60.623
PRIMER_RIGHT_45_TM=61.023
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.2000
PRIMER_RIGHT_45_END_STABILITY=8.1000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=805
PRIMER_PAIR_46_PENALTY=1.648674
PRIMER_LEFT_46_PENALTY=0.910027
PRIMER_RIGHT_46_PENALTY=0.738647
PRIMER_LEFT_46_SEQUENCE=CCAAGGGAATGGTTTGGATT
PRIMER_RIGHT_46_SEQUENCE=AAAAAGTCCCCCAATTCCTC
PRIMER_LEFT_46=789,20
PRIMER_RIGHT_46=1204,20
PRIMER_LEFT_46_TM=60.910
PRIMER_RIGHT_46_TM=59.261
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.1000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=416
PRIMER_PAIR_47_PENALTY=1.648674
PRIMER_LEFT_47_PENALTY=0.910027
PRIMER_RIGHT_47_PENALTY=0.738647
PRIMER_LEFT_47_SEQUENCE=CCAAGGGAATGGTTTGGATT
PRIMER_RIGHT_47_SEQUENCE=GAAAAAGTCCCCCAATTCCT
PRIMER_LEFT_47=789,20
PRIMER_RIGHT_47=1205,20
PRIMER_LEFT_47_TM=60.910
PRIMER_RIGHT_47_TM=59.261
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=417
PRIMER_PAIR_48_PENALTY=1.671499
PRIMER_LEFT_48_PENALTY=0.910027
PRIMER_RIGHT_48_PENALTY=0.761472
PRIMER_LEFT_48_SEQUENCE=CCAAGGGAATGGTTTGGATT
PRIMER_RIGHT_48_SEQUENCE=CCAATTCCTCTCCTGATGCT
PRIMER_LEFT_48=789,20
PRIMER_RIGHT_48=1194,20
PRIMER_LEFT_48_TM=60.910
PRIMER_RIGHT_48_TM=59.239
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.1000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=406
PRIMER_PAIR_49_PENALTY=1.692014
PRIMER_LEFT_49_PENALTY=0.953367
PRIMER_RIGHT_49_PENALTY=0.738647
PRIMER_LEFT_49_SEQUENCE=TCAAGGAAACGACAAGCAAG
PRIMER_RIGHT_49_SEQUENCE=AAAAAGTCCCCCAATTCCTC
PRIMER_LEFT_49=585,20
PRIMER_RIGHT_49=1204,20
PRIMER_LEFT_49_TM=59.047
PRIMER_RIGHT_49_TM=59.261
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=620
=
SEQUENCE_ID=MAGIC.175_4_10908069_10908075_wu-0_bur-0_66237_164_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=GATGCTTTTNGAATTCGCNGTATTGCTATTGCTTCTGTTGCTTTTGTTAGACTCTGTTTTGGAGAAGAAAGANTTGTTTCCACTACTCTTGTGAGAGAGACTCTTAGAGTTAGAGGAAGAGAAGCCACGATGATGATTTTGATTATGATGNGCATTTCTTGATTTATGCAATAGCATGTCGAAATCGAGCGATGGTGTGCAAGCACGTCCTTCGCCGCAAGATTTGCTTCTTCGAAACGCAGTGGCNGCATCTTGAACGAGCATTAGGTCAAGAACTTGCTCAGTNGTTCTGTCCTTCCAGTTGTGAATAGAGCTATTTCTAGGTGACGTNGCTGCTGAGTTTGGGAGGCTGCAGCTCACAAACTTCGTCTTCATTTGATGATCNNNNNNNNNGTCATCGATGTCTCTCGGACGTGAGACTATCATATCGAAGCTCCCATTCCTNGACAATAGCGATTGCGGATCAACCGAGATCCGCCTCTCGGATACGGATTGCATACTCGTCTTCTTGATAAGAATCTTGGGCTCGTCTCCACCACCTAGAACCTCAAAGTCATGCCAGTCCTCGAGACTACTATCGTCCCTGAAGCTGAGAACATCTGTNNCCATTTGTTNCTCTTTGGCCTGNAANNGAATGAGGACTAATAAGAAAATGAAAATATTGGCAAAANGNCTAANACAGATTGATTGATACTTAAATACGGAGCAGGATGGGTTGCACGTCGGTGNCTACNTTAGTCATGACTTGCACGTTTAATATAATATAACCCAAAGTGTTGTTGACTTCATCTNCTAAAAAANGAAAAATGNNTCATNNTTTTTTTATTNGTTAANTATCNCANTATCGTTGACATGAGACCATNCCCANGATCAAGACAAGATCAAAAAGATAGATCTGCCTGCACGAACTACCTAATTATAATATTACCAAAGNATTTGGAGGGTNGTAATCTGCATCAGTGAAGACCATAAGTAGAAGCCATAAATCTTGAATACNTCTNCNNNTNNATGNACNTNGTNCTNTATNNNNTACNTNNTACTTGGNNANTTNTTTNTNNTTGGTNACNTNTNNTTNTCNTTGNTNACTNTNCNCGNNANACTTNGNTATANNNCTTNNTNATANNNCTTNTNNNTNNNTCTNNTATTTTTNNNTTNNNNTNNNTTTTNCNNNNNNNNNNNNNNNNNCTTTGAATTTTNNTGTGTCTTTTNCTACAAATATTTCTTCACTTTCATTGAAATTCATCNCCTTCAATGGATGCACNTACGTCCGTTAGTCATNNNTGGCTTTAGGCCTCATATNTATTCTAGNTGCTCTAGTATATCCCTCNAAAATGGACGGTGAGAAACAATACCAATTTTTNATCCCAAGGGATTTGAACCGGTTATGTTTGAATAACATAGNGATTTGAACGTCAAATCAACAANTTACGAAACGATTAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGCTAAAACATTTTTGAANGTTTTCAAATATGGATTTGATCGACAAANATAGAAGTCATATACTCCTTGATTTTTCCTTGGGGATTGGTGNATCCTTCATTTNCATTGTAGGNGACGNTCATCATNCGTGNAAGATCAATGGTTGCCTTAAGAAGAAAATGTGGCACGCCAGTTGTTGNCAAGAACTCCTCATTTAGTGTCTTATTGGCANCTNCAACNATTTTANNAAGCTCCCTAAAAGNTTCCTCTTTGGTAACCCCATATTGCTTCATATANCAGTTNACCGCGTTCGNGANATATCCTCTACTCATGTCNTCCTACTCAATATTTGNGTAAGTNGGTAAGATTTACAAGAAAGCAGTTATACGCGAGATGTAAAGAAANNATTTATGTATTTGTACCTCATAACCCATNAAATCATTNATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.350503
PRIMER_LEFT_0_PENALTY=0.083883
PRIMER_RIGHT_0_PENALTY=0.266620
PRIMER_LEFT_0_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_0_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_0=695,20
PRIMER_RIGHT_0=1666,20
PRIMER_LEFT_0_TM=59.916
PRIMER_RIGHT_0_TM=59.733
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=972
PRIMER_PAIR_1_PENALTY=0.541950
PRIMER_LEFT_1_PENALTY=0.285121
PRIMER_RIGHT_1_PENALTY=0.256829
PRIMER_LEFT_1_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_1_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_1=893,20
PRIMER_RIGHT_1=1748,20
PRIMER_LEFT_1_TM=60.285
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=0.551741
PRIMER_LEFT_2_PENALTY=0.285121
PRIMER_RIGHT_2_PENALTY=0.266620
PRIMER_LEFT_2_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_2_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_2=893,20
PRIMER_RIGHT_2=1666,20
PRIMER_LEFT_2_TM=60.285
PRIMER_RIGHT_2_TM=59.733
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=774
PRIMER_PAIR_3_PENALTY=0.566115
PRIMER_LEFT_3_PENALTY=0.083883
PRIMER_RIGHT_3_PENALTY=0.482233
PRIMER_LEFT_3_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_3_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_3=695,20
PRIMER_RIGHT_3=1667,20
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=60.482
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=973
PRIMER_PAIR_4_PENALTY=0.767353
PRIMER_LEFT_4_PENALTY=0.285121
PRIMER_RIGHT_4_PENALTY=0.482233
PRIMER_LEFT_4_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_4_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_4=893,20
PRIMER_RIGHT_4=1667,20
PRIMER_LEFT_4_TM=60.285
PRIMER_RIGHT_4_TM=60.482
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=775
PRIMER_PAIR_5_PENALTY=0.769116
PRIMER_LEFT_5_PENALTY=0.512286
PRIMER_RIGHT_5_PENALTY=0.256829
PRIMER_LEFT_5_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_5_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_5=896,20
PRIMER_RIGHT_5=1748,20
PRIMER_LEFT_5_TM=59.488
PRIMER_RIGHT_5_TM=60.257
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.5000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=853
PRIMER_PAIR_6_PENALTY=0.778907
PRIMER_LEFT_6_PENALTY=0.512286
PRIMER_RIGHT_6_PENALTY=0.266620
PRIMER_LEFT_6_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_6_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_6=896,20
PRIMER_RIGHT_6=1666,20
PRIMER_LEFT_6_TM=59.488
PRIMER_RIGHT_6_TM=59.733
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=771
PRIMER_PAIR_7_PENALTY=0.979374
PRIMER_LEFT_7_PENALTY=0.712754
PRIMER_RIGHT_7_PENALTY=0.266620
PRIMER_LEFT_7_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_7_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_7=697,20
PRIMER_RIGHT_7=1666,20
PRIMER_LEFT_7_TM=60.713
PRIMER_RIGHT_7_TM=59.733
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=970
PRIMER_PAIR_8_PENALTY=0.979374
PRIMER_LEFT_8_PENALTY=0.712754
PRIMER_RIGHT_8_PENALTY=0.266620
PRIMER_LEFT_8_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_8_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_8=696,20
PRIMER_RIGHT_8=1666,20
PRIMER_LEFT_8_TM=60.713
PRIMER_RIGHT_8_TM=59.733
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=0.994519
PRIMER_LEFT_9_PENALTY=0.512286
PRIMER_RIGHT_9_PENALTY=0.482233
PRIMER_LEFT_9_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_9_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_9=896,20
PRIMER_RIGHT_9=1667,20
PRIMER_LEFT_9_TM=59.488
PRIMER_RIGHT_9_TM=60.482
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.5000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=772
PRIMER_PAIR_10_PENALTY=1.194986
PRIMER_LEFT_10_PENALTY=0.712754
PRIMER_RIGHT_10_PENALTY=0.482233
PRIMER_LEFT_10_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_10_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_10=697,20
PRIMER_RIGHT_10=1667,20
PRIMER_LEFT_10_TM=60.713
PRIMER_RIGHT_10_TM=60.482
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=971
PRIMER_PAIR_11_PENALTY=1.194986
PRIMER_LEFT_11_PENALTY=0.712754
PRIMER_RIGHT_11_PENALTY=0.482233
PRIMER_LEFT_11_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_11_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_11=696,20
PRIMER_RIGHT_11=1667,20
PRIMER_LEFT_11_TM=60.713
PRIMER_RIGHT_11_TM=60.482
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=1.227016
PRIMER_LEFT_12_PENALTY=0.083883
PRIMER_RIGHT_12_PENALTY=1.143133
PRIMER_LEFT_12_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_12_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_12=695,20
PRIMER_RIGHT_12=1662,20
PRIMER_LEFT_12_TM=59.916
PRIMER_RIGHT_12_TM=61.143
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=968
PRIMER_PAIR_13_PENALTY=1.227016
PRIMER_LEFT_13_PENALTY=0.083883
PRIMER_RIGHT_13_PENALTY=1.143133
PRIMER_LEFT_13_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_13_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_13=695,20
PRIMER_RIGHT_13=1663,20
PRIMER_LEFT_13_TM=59.916
PRIMER_RIGHT_13_TM=61.143
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=969
PRIMER_PAIR_14_PENALTY=1.239333
PRIMER_LEFT_14_PENALTY=0.083883
PRIMER_RIGHT_14_PENALTY=1.155450
PRIMER_LEFT_14_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_14_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_14=695,20
PRIMER_RIGHT_14=1352,21
PRIMER_LEFT_14_TM=59.916
PRIMER_RIGHT_14_TM=59.845
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=658
PRIMER_PAIR_15_PENALTY=1.239333
PRIMER_LEFT_15_PENALTY=0.083883
PRIMER_RIGHT_15_PENALTY=1.155450
PRIMER_LEFT_15_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_15_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_15=695,20
PRIMER_RIGHT_15=1353,21
PRIMER_LEFT_15_TM=59.916
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=659
PRIMER_PAIR_16_PENALTY=1.252490
PRIMER_LEFT_16_PENALTY=0.995660
PRIMER_RIGHT_16_PENALTY=0.256829
PRIMER_LEFT_16_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_16_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_16=894,20
PRIMER_RIGHT_16=1748,20
PRIMER_LEFT_16_TM=60.996
PRIMER_RIGHT_16_TM=60.257
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=855
PRIMER_PAIR_17_PENALTY=1.262281
PRIMER_LEFT_17_PENALTY=0.995660
PRIMER_RIGHT_17_PENALTY=0.266620
PRIMER_LEFT_17_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_17_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_17=894,20
PRIMER_RIGHT_17=1666,20
PRIMER_LEFT_17_TM=60.996
PRIMER_RIGHT_17_TM=59.733
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=773
PRIMER_PAIR_18_PENALTY=1.428253
PRIMER_LEFT_18_PENALTY=0.285121
PRIMER_RIGHT_18_PENALTY=1.143133
PRIMER_LEFT_18_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_18_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_18=893,20
PRIMER_RIGHT_18=1662,20
PRIMER_LEFT_18_TM=60.285
PRIMER_RIGHT_18_TM=61.143
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=770
PRIMER_PAIR_19_PENALTY=1.428253
PRIMER_LEFT_19_PENALTY=0.285121
PRIMER_RIGHT_19_PENALTY=1.143133
PRIMER_LEFT_19_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_19_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_19=893,20
PRIMER_RIGHT_19=1663,20
PRIMER_LEFT_19_TM=60.285
PRIMER_RIGHT_19_TM=61.143
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=771
PRIMER_PAIR_20_PENALTY=1.440571
PRIMER_LEFT_20_PENALTY=0.285121
PRIMER_RIGHT_20_PENALTY=1.155450
PRIMER_LEFT_20_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_20_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_20=893,20
PRIMER_RIGHT_20=1352,21
PRIMER_LEFT_20_TM=60.285
PRIMER_RIGHT_20_TM=59.845
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=460
PRIMER_PAIR_21_PENALTY=1.440571
PRIMER_LEFT_21_PENALTY=0.285121
PRIMER_RIGHT_21_PENALTY=1.155450
PRIMER_LEFT_21_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_21_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_21=893,20
PRIMER_RIGHT_21=1353,21
PRIMER_LEFT_21_TM=60.285
PRIMER_RIGHT_21_TM=59.845
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=461
PRIMER_PAIR_22_PENALTY=1.465064
PRIMER_LEFT_22_PENALTY=0.083883
PRIMER_RIGHT_22_PENALTY=1.381181
PRIMER_LEFT_22_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_22_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_22=695,20
PRIMER_RIGHT_22=1661,20
PRIMER_LEFT_22_TM=59.916
PRIMER_RIGHT_22_TM=58.619
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=967
PRIMER_PAIR_23_PENALTY=1.477893
PRIMER_LEFT_23_PENALTY=0.995660
PRIMER_RIGHT_23_PENALTY=0.482233
PRIMER_LEFT_23_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_23_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_23=894,20
PRIMER_RIGHT_23=1667,20
PRIMER_LEFT_23_TM=60.996
PRIMER_RIGHT_23_TM=60.482
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=774
PRIMER_PAIR_24_PENALTY=1.581829
PRIMER_LEFT_24_PENALTY=0.426379
PRIMER_RIGHT_24_PENALTY=1.155450
PRIMER_LEFT_24_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_24_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_24=527,20
PRIMER_RIGHT_24=1352,21
PRIMER_LEFT_24_TM=59.574
PRIMER_RIGHT_24_TM=59.845
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=826
PRIMER_PAIR_25_PENALTY=1.581829
PRIMER_LEFT_25_PENALTY=0.426379
PRIMER_RIGHT_25_PENALTY=1.155450
PRIMER_LEFT_25_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_25_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_25=527,20
PRIMER_RIGHT_25=1353,21
PRIMER_LEFT_25_TM=59.574
PRIMER_RIGHT_25_TM=59.845
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=827
PRIMER_PAIR_26_PENALTY=1.637322
PRIMER_LEFT_26_PENALTY=0.083883
PRIMER_RIGHT_26_PENALTY=1.553439
PRIMER_LEFT_26_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_26_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_26=695,20
PRIMER_RIGHT_26=1661,21
PRIMER_LEFT_26_TM=59.916
PRIMER_RIGHT_26_TM=59.447
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=967
PRIMER_PAIR_27_PENALTY=1.655419
PRIMER_LEFT_27_PENALTY=0.512286
PRIMER_RIGHT_27_PENALTY=1.143133
PRIMER_LEFT_27_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_27_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_27=896,20
PRIMER_RIGHT_27=1662,20
PRIMER_LEFT_27_TM=59.488
PRIMER_RIGHT_27_TM=61.143
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.5000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=767
PRIMER_PAIR_28_PENALTY=1.655419
PRIMER_LEFT_28_PENALTY=0.512286
PRIMER_RIGHT_28_PENALTY=1.143133
PRIMER_LEFT_28_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_28_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_28=896,20
PRIMER_RIGHT_28=1663,20
PRIMER_LEFT_28_TM=59.488
PRIMER_RIGHT_28_TM=61.143
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.5000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=768
PRIMER_PAIR_29_PENALTY=1.666302
PRIMER_LEFT_29_PENALTY=0.285121
PRIMER_RIGHT_29_PENALTY=1.381181
PRIMER_LEFT_29_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_29_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_29=893,20
PRIMER_RIGHT_29=1661,20
PRIMER_LEFT_29_TM=60.285
PRIMER_RIGHT_29_TM=58.619
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=769
PRIMER_PAIR_30_PENALTY=1.667737
PRIMER_LEFT_30_PENALTY=0.512286
PRIMER_RIGHT_30_PENALTY=1.155450
PRIMER_LEFT_30_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_30_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_30=896,20
PRIMER_RIGHT_30=1352,21
PRIMER_LEFT_30_TM=59.488
PRIMER_RIGHT_30_TM=59.845
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.5000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=457
PRIMER_PAIR_31_PENALTY=1.667737
PRIMER_LEFT_31_PENALTY=0.512286
PRIMER_RIGHT_31_PENALTY=1.155450
PRIMER_LEFT_31_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_31_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_31=896,20
PRIMER_RIGHT_31=1353,21
PRIMER_LEFT_31_TM=59.488
PRIMER_RIGHT_31_TM=59.845
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=458
PRIMER_PAIR_32_PENALTY=1.669226
PRIMER_LEFT_32_PENALTY=0.513776
PRIMER_RIGHT_32_PENALTY=1.155450
PRIMER_LEFT_32_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_32_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_32=423,20
PRIMER_RIGHT_32=1352,21
PRIMER_LEFT_32_TM=59.486
PRIMER_RIGHT_32_TM=59.845
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=930
PRIMER_PAIR_33_PENALTY=1.669226
PRIMER_LEFT_33_PENALTY=0.513776
PRIMER_RIGHT_33_PENALTY=1.155450
PRIMER_LEFT_33_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_33_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_33=423,20
PRIMER_RIGHT_33=1353,21
PRIMER_LEFT_33_TM=59.486
PRIMER_RIGHT_33_TM=59.845
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=1.703379
PRIMER_LEFT_34_PENALTY=1.446550
PRIMER_RIGHT_34_PENALTY=0.256829
PRIMER_LEFT_34_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_34_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_34=895,21
PRIMER_RIGHT_34=1748,20
PRIMER_LEFT_34_TM=60.447
PRIMER_RIGHT_34_TM=60.257
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=854
PRIMER_PAIR_35_PENALTY=1.713170
PRIMER_LEFT_35_PENALTY=1.446550
PRIMER_RIGHT_35_PENALTY=0.266620
PRIMER_LEFT_35_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_35_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_35=895,21
PRIMER_RIGHT_35=1666,20
PRIMER_LEFT_35_TM=60.447
PRIMER_RIGHT_35_TM=59.733
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.5000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=772
PRIMER_PAIR_36_PENALTY=1.740964
PRIMER_LEFT_36_PENALTY=1.484135
PRIMER_RIGHT_36_PENALTY=0.256829
PRIMER_LEFT_36_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_36_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_36=891,21
PRIMER_RIGHT_36=1748,20
PRIMER_LEFT_36_TM=59.516
PRIMER_RIGHT_36_TM=60.257
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.1000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=858
PRIMER_PAIR_37_PENALTY=1.750755
PRIMER_LEFT_37_PENALTY=1.484135
PRIMER_RIGHT_37_PENALTY=0.266620
PRIMER_LEFT_37_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_37_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_37=891,21
PRIMER_RIGHT_37=1666,20
PRIMER_LEFT_37_TM=59.516
PRIMER_RIGHT_37_TM=59.733
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.1000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=776
PRIMER_PAIR_38_PENALTY=1.785200
PRIMER_LEFT_38_PENALTY=1.528370
PRIMER_RIGHT_38_PENALTY=0.256829
PRIMER_LEFT_38_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_38_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_38=892,20
PRIMER_RIGHT_38=1748,20
PRIMER_LEFT_38_TM=58.472
PRIMER_RIGHT_38_TM=60.257
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.1000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=857
PRIMER_PAIR_39_PENALTY=1.794991
PRIMER_LEFT_39_PENALTY=1.528370
PRIMER_RIGHT_39_PENALTY=0.266620
PRIMER_LEFT_39_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_39_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_39=892,20
PRIMER_RIGHT_39=1666,20
PRIMER_LEFT_39_TM=58.472
PRIMER_RIGHT_39_TM=59.733
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.1000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=775
PRIMER_PAIR_40_PENALTY=1.811843
PRIMER_LEFT_40_PENALTY=0.656393
PRIMER_RIGHT_40_PENALTY=1.155450
PRIMER_LEFT_40_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_40_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_40=545,20
PRIMER_RIGHT_40=1352,21
PRIMER_LEFT_40_TM=60.656
PRIMER_RIGHT_40_TM=59.845
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=808
PRIMER_PAIR_41_PENALTY=1.811843
PRIMER_LEFT_41_PENALTY=0.656393
PRIMER_RIGHT_41_PENALTY=1.155450
PRIMER_LEFT_41_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_41_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_41=545,20
PRIMER_RIGHT_41=1353,21
PRIMER_LEFT_41_TM=60.656
PRIMER_RIGHT_41_TM=59.845
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=809
PRIMER_PAIR_42_PENALTY=1.838560
PRIMER_LEFT_42_PENALTY=0.285121
PRIMER_RIGHT_42_PENALTY=1.553439
PRIMER_LEFT_42_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_42_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_42=893,20
PRIMER_RIGHT_42=1661,21
PRIMER_LEFT_42_TM=60.285
PRIMER_RIGHT_42_TM=59.447
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=769
PRIMER_PAIR_43_PENALTY=1.855887
PRIMER_LEFT_43_PENALTY=0.712754
PRIMER_RIGHT_43_PENALTY=1.143133
PRIMER_LEFT_43_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_43_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_43=697,20
PRIMER_RIGHT_43=1662,20
PRIMER_LEFT_43_TM=60.713
PRIMER_RIGHT_43_TM=61.143
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=1.855887
PRIMER_LEFT_44_PENALTY=0.712754
PRIMER_RIGHT_44_PENALTY=1.143133
PRIMER_LEFT_44_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_44_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_44=697,20
PRIMER_RIGHT_44=1663,20
PRIMER_LEFT_44_TM=60.713
PRIMER_RIGHT_44_TM=61.143
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=967
PRIMER_PAIR_45_PENALTY=1.855887
PRIMER_LEFT_45_PENALTY=0.712754
PRIMER_RIGHT_45_PENALTY=1.143133
PRIMER_LEFT_45_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_45_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_45=696,20
PRIMER_RIGHT_45=1662,20
PRIMER_LEFT_45_TM=60.713
PRIMER_RIGHT_45_TM=61.143
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=967
PRIMER_PAIR_46_PENALTY=1.855887
PRIMER_LEFT_46_PENALTY=0.712754
PRIMER_RIGHT_46_PENALTY=1.143133
PRIMER_LEFT_46_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_46_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_46=696,20
PRIMER_RIGHT_46=1663,20
PRIMER_LEFT_46_TM=60.713
PRIMER_RIGHT_46_TM=61.143
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=968
PRIMER_PAIR_47_PENALTY=1.868204
PRIMER_LEFT_47_PENALTY=0.712754
PRIMER_RIGHT_47_PENALTY=1.155450
PRIMER_LEFT_47_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_47_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_47=697,20
PRIMER_RIGHT_47=1352,21
PRIMER_LEFT_47_TM=60.713
PRIMER_RIGHT_47_TM=59.845
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=656
PRIMER_PAIR_48_PENALTY=1.868204
PRIMER_LEFT_48_PENALTY=0.712754
PRIMER_RIGHT_48_PENALTY=1.155450
PRIMER_LEFT_48_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_48_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_48=697,20
PRIMER_RIGHT_48=1353,21
PRIMER_LEFT_48_TM=60.713
PRIMER_RIGHT_48_TM=59.845
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=657
PRIMER_PAIR_49_PENALTY=1.868204
PRIMER_LEFT_49_PENALTY=0.712754
PRIMER_RIGHT_49_PENALTY=1.155450
PRIMER_LEFT_49_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_49_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_49=696,20
PRIMER_RIGHT_49=1352,21
PRIMER_LEFT_49_TM=60.713
PRIMER_RIGHT_49_TM=59.845
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=657
=
SEQUENCE_ID=MAGIC.446_2_5152902_5153071_oy-0_sf-2_23409_13316_TRUE
SEQUENCE_TARGET=950,269
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATATNNNNNNNNATATNNNNATANTNATNNAAAGAACTCTAAATACTTTGTACNATCAAAANTNANATTATNAACTATTTTTTCCAACATTGAACTATATGGTAAGAAATTANATNAATNTAATTATTAATTATACNTGTGAAATTTNATNANGNAANAAGATACNCAGNTACANNANGTANAANNGAATACATNGNCACANATGCNAANTTNNNAAATGTANAACNAACTACATGANNTACATC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_14013828_14014151_bur-0_rsch-4_8746_19759_TRUE
SEQUENCE_TARGET=950,423
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACNNGATATGATCTTATAGACGANAANAGCTTGCTGAAGCATTCACCCAAATGAGTCTTGAACGAACTACTCCCCCAGGTATGAAATATCGTTTGACAGGTTCGAGGACCTCTAGAAACCAAGAGAAGACTTTGGAGCTTCAANAGATGGATNTTCAAGTGTCATCTNNNAAAGTGATCGAGAGAGTCAAGATCNAGANCAANACNTCANGGACATGGAANAGCNCGAAGACATTNATNAANCNNCTCAAGAGGAACTCCAGAAAGACCCCTACTTCNAGGNACCAGNNNNCAAACGAGCACAAGACTTGATTCCNNNNNNNNNNNNAGAAGTACTACAAGAAGTAACTCAGGCGACATCTATTGGGCCTAAAANACATCTAGGTAAGGTTTATTCAGTTCATATTATCTTTGNAGGTCCACACAGCCCAGACAACGGCTAGNGTCGCCNNNNATCGTGCTCCGCAAGAGAACTTAATTAGTTTCCTTATTTTAGCTTATTTTCTTTNTAAAAACAATGGTTTTTGTGTTTTGATTNTATTTGCTTCCTTNTTGCNTTTTTACATTTAATTTCAGCCCTNGGNNNNGCTGAAGGACTCTTATAAAGTCACCCTACATCAGTTGNTTNNNNNNNACCTTTGATTATNAATAAANAACCGCATTTTCTNACTTCTTCTNTTTAAGTGTTCTTAAGAGATTAAGCTNGTTCTTTGTCTTGTGTGTGTGATTNACCAAGNCTAATTTNGTGTACTGTATTAGAGACTTATCANATCTTTGTGGTGTACTTCAATCCGCCNGCANTTCCCCTCCATTANATNCCACANNANAGANGTGATCGCCATCGTGTTANANNNNNNNNNNNNNNCATCTTTCCTNCACAAGAGAGAAGCGACCGCCAGCTTGTGAAGCCACCATCTCTATCAGATACAAAGCTCACCNCCGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_2_6608658_6608712_ct-1_hi-0_50891_110338_TRUE
SEQUENCE_TARGET=950,154
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAAAACTTTNAGTACAGTCTACAANTATAGTNNNNGAATCACTCNTCTTCGACTTAAAAAANGATNNNNNNNNAAACNCAAATTTCCTAAAAGAATAGTAAGTTAGTNACACACACATTGTAGTNNNTTTTNATGANAAAATAAAAGNAAGACCNNNNNNNNNNNNNNNNNNNNNNNNNNAAAANAAAAATACCAANTCGCATTTGTACAAAACTTAGATAATGNGACACTGCCGAAGAAAAGGTCTTTGATTGACNAATCACNATCTTGATTATNTTTCTCACAAATCCATTTATTTTCTNTATAAATANATCCATCTAAATCAAAACAGATNATTGTCCNNNNNNNNNNNCAAAATACAAAGATGACGTTTCACAAAATCTTTTTNGTAATCTCTCTGATGCTCGTTGTCTCNTTAGTTAATGCTGTTGACTACTCGGTCGTCGACAATTCCGGTGACTCTACCGGAGGAAGGAGATTCCGGGGAGAGATCGGNGGCATAAGCTACGGCACACAGACACTAAGATCTGCGACAGACTTTGTGTGGCGTTTNTTCCAACAAACCAACCCTTCAGACAGAAAGTCAGTTACAAAGATCACACTGTTCATGGAGAACGGTGACGGAGTAGCGTACAACTCTGNAAACGAGATACATTTCAACGTGGGATATCTCGCGGGTGTNTCNGGAGATGTNAAGAGGGAATTTACCGGTGTAGTTTATCATGAGGTGGTCCATTCTTGGCAGTGGAACGGTGNGGGTCGGGCTCCGGGTGGGCTTATCGAAGGGATANCGGATTACGTGAGGCTGAAAGCGGGTTACGCTCCAAGTCATTGGGTCGGGCCTGGTCGTGGTGATAGGTGGGATCAAGGTTATGACGTTACCGCGAGGTTCTTNGATTATTGTAACGGTTTNAGGAATGGGTTTGTGGCCGAGCTGAACAAGAAGATGAGGAATGGTTATAGTGATGGCTTCTTCGTTGATTTGCTNNGGAAAGACGTGAATCAATTATGGAGAGANTACAAGGCCAAGTATGGTCAATGAATACAAACGNNNNAAAATAATCTCAATATGGTAATGAAAATAAAATAAAATGGTGAATAAAATGAAAAATATGGTGTTGTTAAGTTTCTATTGGAAAACACATACATTACACTCTTTGTATTAAAATATAAGCTCTTGCTAAAAATGGTATTGTATGTTTAGTCAAAGANAATATATTGGGGGTTGATTTAGAANGTTATGTCACTGTTTATTCGTNATCGTGGCCGAATAAANACTCTTTCTTGTNATTGTNCATTAAGNTATGTGAGAAGAAAAAAGAAGTAAAATCTATGGGAATATAAAGTTAACGTCTCGCAANAATTAAACGACATGATTAAATAGGTG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.792952
PRIMER_LEFT_0_PENALTY=0.667126
PRIMER_RIGHT_0_PENALTY=0.125826
PRIMER_LEFT_0_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_0_SEQUENCE=GCTACTCCGTCACCGTTCTC
PRIMER_LEFT_0=893,20
PRIMER_RIGHT_0=1296,20
PRIMER_LEFT_0_TM=59.333
PRIMER_RIGHT_0_TM=59.874
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=404
PRIMER_PAIR_1_PENALTY=0.945973
PRIMER_LEFT_1_PENALTY=0.667126
PRIMER_RIGHT_1_PENALTY=0.278847
PRIMER_LEFT_1_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_1_SEQUENCE=CTACTCCGTCACCGTTCTCC
PRIMER_LEFT_1=893,20
PRIMER_RIGHT_1=1295,20
PRIMER_LEFT_1_TM=59.333
PRIMER_RIGHT_1_TM=59.721
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=403
PRIMER_PAIR_2_PENALTY=1.006356
PRIMER_LEFT_2_PENALTY=0.667126
PRIMER_RIGHT_2_PENALTY=0.339231
PRIMER_LEFT_2_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_2_SEQUENCE=ATCCCACCTATCACCACGAC
PRIMER_LEFT_2=893,20
PRIMER_RIGHT_2=1531,20
PRIMER_LEFT_2_TM=59.333
PRIMER_RIGHT_2_TM=59.661
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=639
PRIMER_PAIR_3_PENALTY=1.006356
PRIMER_LEFT_3_PENALTY=0.667126
PRIMER_RIGHT_3_PENALTY=0.339231
PRIMER_LEFT_3_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_3_SEQUENCE=ATCCCACCTATCACCACGAC
PRIMER_LEFT_3=892,20
PRIMER_RIGHT_3=1531,20
PRIMER_LEFT_3_TM=59.333
PRIMER_RIGHT_3_TM=59.661
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=640
PRIMER_PAIR_4_PENALTY=1.209822
PRIMER_LEFT_4_PENALTY=0.667126
PRIMER_RIGHT_4_PENALTY=0.542697
PRIMER_LEFT_4_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_4_SEQUENCE=AGCAAATCAACGAAGAAGCC
PRIMER_LEFT_4=893,20
PRIMER_RIGHT_4=1653,20
PRIMER_LEFT_4_TM=59.333
PRIMER_RIGHT_4_TM=59.457
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=761
PRIMER_PAIR_5_PENALTY=1.209822
PRIMER_LEFT_5_PENALTY=0.667126
PRIMER_RIGHT_5_PENALTY=0.542697
PRIMER_LEFT_5_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_5_SEQUENCE=AGCAAATCAACGAAGAAGCC
PRIMER_LEFT_5=892,20
PRIMER_RIGHT_5=1653,20
PRIMER_LEFT_5_TM=59.333
PRIMER_RIGHT_5_TM=59.457
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=762
PRIMER_PAIR_6_PENALTY=1.414194
PRIMER_LEFT_6_PENALTY=0.667126
PRIMER_RIGHT_6_PENALTY=0.747069
PRIMER_LEFT_6_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_6_SEQUENCE=GATCCCACCTATCACCACGA
PRIMER_LEFT_6=893,20
PRIMER_RIGHT_6=1532,20
PRIMER_LEFT_6_TM=59.333
PRIMER_RIGHT_6_TM=60.747
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=640
PRIMER_PAIR_7_PENALTY=1.414194
PRIMER_LEFT_7_PENALTY=0.667126
PRIMER_RIGHT_7_PENALTY=0.747069
PRIMER_LEFT_7_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_7_SEQUENCE=GATCCCACCTATCACCACGA
PRIMER_LEFT_7=892,20
PRIMER_RIGHT_7=1532,20
PRIMER_LEFT_7_TM=59.333
PRIMER_RIGHT_7_TM=60.747
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=641
PRIMER_PAIR_8_PENALTY=1.453631
PRIMER_LEFT_8_PENALTY=0.667126
PRIMER_RIGHT_8_PENALTY=0.786506
PRIMER_LEFT_8_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_8_SEQUENCE=CCTTGATCCCACCTATCACC
PRIMER_LEFT_8=893,20
PRIMER_RIGHT_8=1536,20
PRIMER_LEFT_8_TM=59.333
PRIMER_RIGHT_8_TM=59.213
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=644
PRIMER_PAIR_9_PENALTY=1.453631
PRIMER_LEFT_9_PENALTY=0.667126
PRIMER_RIGHT_9_PENALTY=0.786506
PRIMER_LEFT_9_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_9_SEQUENCE=CCTTGATCCCACCTATCACC
PRIMER_LEFT_9=892,20
PRIMER_RIGHT_9=1536,20
PRIMER_LEFT_9_TM=59.333
PRIMER_RIGHT_9_TM=59.213
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=645
PRIMER_PAIR_10_PENALTY=1.668830
PRIMER_LEFT_10_PENALTY=0.667126
PRIMER_RIGHT_10_PENALTY=1.001704
PRIMER_LEFT_10_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_10_SEQUENCE=TATCCCTTCGATAAGCCCAC
PRIMER_LEFT_10=893,20
PRIMER_RIGHT_10=1456,20
PRIMER_LEFT_10_TM=59.333
PRIMER_RIGHT_10_TM=58.998
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=564
PRIMER_PAIR_11_PENALTY=1.668830
PRIMER_LEFT_11_PENALTY=0.667126
PRIMER_RIGHT_11_PENALTY=1.001704
PRIMER_LEFT_11_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_11_SEQUENCE=TATCCCTTCGATAAGCCCAC
PRIMER_LEFT_11=892,20
PRIMER_RIGHT_11=1456,20
PRIMER_LEFT_11_TM=59.333
PRIMER_RIGHT_11_TM=58.998
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=565
PRIMER_PAIR_12_PENALTY=1.687831
PRIMER_LEFT_12_PENALTY=0.667126
PRIMER_RIGHT_12_PENALTY=1.020705
PRIMER_LEFT_12_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_12_SEQUENCE=CCCAATGACTTGGAGCGTAA
PRIMER_LEFT_12=893,20
PRIMER_RIGHT_12=1502,20
PRIMER_LEFT_12_TM=59.333
PRIMER_RIGHT_12_TM=61.021
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=7.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=610
PRIMER_PAIR_13_PENALTY=1.687831
PRIMER_LEFT_13_PENALTY=0.667126
PRIMER_RIGHT_13_PENALTY=1.020705
PRIMER_LEFT_13_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_13_SEQUENCE=CCCAATGACTTGGAGCGTAA
PRIMER_LEFT_13=892,20
PRIMER_RIGHT_13=1502,20
PRIMER_LEFT_13_TM=59.333
PRIMER_RIGHT_13_TM=61.021
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=7.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=611
PRIMER_PAIR_14_PENALTY=1.713092
PRIMER_LEFT_14_PENALTY=0.667126
PRIMER_RIGHT_14_PENALTY=1.045967
PRIMER_LEFT_14_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_14_SEQUENCE=TTCCACTGCCAAGAATGGAC
PRIMER_LEFT_14=893,20
PRIMER_RIGHT_14=1416,20
PRIMER_LEFT_14_TM=59.333
PRIMER_RIGHT_14_TM=61.046
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=524
PRIMER_PAIR_15_PENALTY=1.713092
PRIMER_LEFT_15_PENALTY=0.667126
PRIMER_RIGHT_15_PENALTY=1.045967
PRIMER_LEFT_15_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_15_SEQUENCE=TTCCACTGCCAAGAATGGAC
PRIMER_LEFT_15=892,20
PRIMER_RIGHT_15=1416,20
PRIMER_LEFT_15_TM=59.333
PRIMER_RIGHT_15_TM=61.046
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=525
PRIMER_PAIR_16_PENALTY=1.733626
PRIMER_LEFT_16_PENALTY=0.667126
PRIMER_RIGHT_16_PENALTY=1.066501
PRIMER_LEFT_16_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_16_SEQUENCE=ACCTTGATCCCACCTATCACC
PRIMER_LEFT_16=893,20
PRIMER_RIGHT_16=1537,21
PRIMER_LEFT_16_TM=59.333
PRIMER_RIGHT_16_TM=60.067
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=645
PRIMER_PAIR_17_PENALTY=1.733626
PRIMER_LEFT_17_PENALTY=0.667126
PRIMER_RIGHT_17_PENALTY=1.066501
PRIMER_LEFT_17_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_17_SEQUENCE=ACCTTGATCCCACCTATCACC
PRIMER_LEFT_17=892,20
PRIMER_RIGHT_17=1537,21
PRIMER_LEFT_17_TM=59.333
PRIMER_RIGHT_17_TM=60.067
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=646
PRIMER_PAIR_18_PENALTY=1.865624
PRIMER_LEFT_18_PENALTY=0.667126
PRIMER_RIGHT_18_PENALTY=1.198499
PRIMER_LEFT_18_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_18_SEQUENCE=TTGTACGCTACTCCGTCACC
PRIMER_LEFT_18=893,20
PRIMER_RIGHT_18=1302,20
PRIMER_LEFT_18_TM=59.333
PRIMER_RIGHT_18_TM=58.802
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=410
PRIMER_PAIR_19_PENALTY=1.865624
PRIMER_LEFT_19_PENALTY=0.667126
PRIMER_RIGHT_19_PENALTY=1.198499
PRIMER_LEFT_19_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_19_SEQUENCE=TTGTACGCTACTCCGTCACC
PRIMER_LEFT_19=892,20
PRIMER_RIGHT_19=1302,20
PRIMER_LEFT_19_TM=59.333
PRIMER_RIGHT_19_TM=58.802
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=411
PRIMER_PAIR_20_PENALTY=1.952017
PRIMER_LEFT_20_PENALTY=0.667126
PRIMER_RIGHT_20_PENALTY=1.284892
PRIMER_LEFT_20_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_20_SEQUENCE=GCAAATCAACGAAGAAGCCA
PRIMER_LEFT_20=893,20
PRIMER_RIGHT_20=1652,20
PRIMER_LEFT_20_TM=59.333
PRIMER_RIGHT_20_TM=61.285
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=760
PRIMER_PAIR_21_PENALTY=1.952017
PRIMER_LEFT_21_PENALTY=0.667126
PRIMER_RIGHT_21_PENALTY=1.284892
PRIMER_LEFT_21_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_21_SEQUENCE=GCAAATCAACGAAGAAGCCA
PRIMER_LEFT_21=892,20
PRIMER_RIGHT_21=1652,20
PRIMER_LEFT_21_TM=59.333
PRIMER_RIGHT_21_TM=61.285
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=761
PRIMER_PAIR_22_PENALTY=1.990720
PRIMER_LEFT_22_PENALTY=0.667126
PRIMER_RIGHT_22_PENALTY=1.323594
PRIMER_LEFT_22_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_22_SEQUENCE=GTTGTACGCTACTCCGTCACC
PRIMER_LEFT_22=893,20
PRIMER_RIGHT_22=1303,21
PRIMER_LEFT_22_TM=59.333
PRIMER_RIGHT_22_TM=59.676
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=57.143
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=411
PRIMER_PAIR_23_PENALTY=1.990720
PRIMER_LEFT_23_PENALTY=0.667126
PRIMER_RIGHT_23_PENALTY=1.323594
PRIMER_LEFT_23_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_23_SEQUENCE=GTTGTACGCTACTCCGTCACC
PRIMER_LEFT_23=892,20
PRIMER_RIGHT_23=1303,21
PRIMER_LEFT_23_TM=59.333
PRIMER_RIGHT_23_TM=59.676
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=57.143
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=412
PRIMER_PAIR_24_PENALTY=2.057066
PRIMER_LEFT_24_PENALTY=0.667126
PRIMER_RIGHT_24_PENALTY=1.389940
PRIMER_LEFT_24_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_24_SEQUENCE=CCACCTATCACCACGACCAG
PRIMER_LEFT_24=893,20
PRIMER_RIGHT_24=1528,20
PRIMER_LEFT_24_TM=59.333
PRIMER_RIGHT_24_TM=61.390
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=60.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=636
PRIMER_PAIR_25_PENALTY=2.057066
PRIMER_LEFT_25_PENALTY=0.667126
PRIMER_RIGHT_25_PENALTY=1.389940
PRIMER_LEFT_25_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_25_SEQUENCE=CCACCTATCACCACGACCAG
PRIMER_LEFT_25=892,20
PRIMER_RIGHT_25=1528,20
PRIMER_LEFT_25_TM=59.333
PRIMER_RIGHT_25_TM=61.390
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=60.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=637
PRIMER_PAIR_26_PENALTY=2.071315
PRIMER_LEFT_26_PENALTY=0.667126
PRIMER_RIGHT_26_PENALTY=1.404189
PRIMER_LEFT_26_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_26_SEQUENCE=CATCTTCTTGTTCAGCTCGG
PRIMER_LEFT_26=893,20
PRIMER_RIGHT_26=1615,20
PRIMER_LEFT_26_TM=59.333
PRIMER_RIGHT_26_TM=58.596
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=723
PRIMER_PAIR_27_PENALTY=2.189917
PRIMER_LEFT_27_PENALTY=0.667126
PRIMER_RIGHT_27_PENALTY=1.522792
PRIMER_LEFT_27_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_27_SEQUENCE=TCATCTTCTTGTTCAGCTCGG
PRIMER_LEFT_27=893,20
PRIMER_RIGHT_27=1616,21
PRIMER_LEFT_27_TM=59.333
PRIMER_RIGHT_27_TM=60.523
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=9.9000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=724
PRIMER_PAIR_28_PENALTY=2.223894
PRIMER_LEFT_28_PENALTY=0.667126
PRIMER_RIGHT_28_PENALTY=1.556768
PRIMER_LEFT_28_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_28_SEQUENCE=TCATTGACCATACTTGGCCTT
PRIMER_LEFT_28=892,20
PRIMER_RIGHT_28=1708,21
PRIMER_LEFT_28_TM=59.333
PRIMER_RIGHT_28_TM=59.443
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=817
PRIMER_PAIR_29_PENALTY=2.302219
PRIMER_LEFT_29_PENALTY=2.176393
PRIMER_RIGHT_29_PENALTY=0.125826
PRIMER_LEFT_29_SEQUENCE=GACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_29_SEQUENCE=GCTACTCCGTCACCGTTCTC
PRIMER_LEFT_29=892,21
PRIMER_RIGHT_29=1296,20
PRIMER_LEFT_29_TM=61.176
PRIMER_RIGHT_29_TM=59.874
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=60.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=405
PRIMER_PAIR_30_PENALTY=2.308347
PRIMER_LEFT_30_PENALTY=0.667126
PRIMER_RIGHT_30_PENALTY=1.641222
PRIMER_LEFT_30_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_30_SEQUENCE=CTTGATCCCACCTATCACCA
PRIMER_LEFT_30=893,20
PRIMER_RIGHT_30=1535,20
PRIMER_LEFT_30_TM=59.333
PRIMER_RIGHT_30_TM=58.359
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=643
PRIMER_PAIR_31_PENALTY=2.308347
PRIMER_LEFT_31_PENALTY=0.667126
PRIMER_RIGHT_31_PENALTY=1.641222
PRIMER_LEFT_31_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_31_SEQUENCE=CTTGATCCCACCTATCACCA
PRIMER_LEFT_31=892,20
PRIMER_RIGHT_31=1535,20
PRIMER_LEFT_31_TM=59.333
PRIMER_RIGHT_31_TM=58.359
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=644
PRIMER_PAIR_32_PENALTY=2.322029
PRIMER_LEFT_32_PENALTY=0.667126
PRIMER_RIGHT_32_PENALTY=1.654904
PRIMER_LEFT_32_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_32_SEQUENCE=TACTCCGTCACCGTTCTCCA
PRIMER_LEFT_32=893,20
PRIMER_RIGHT_32=1294,20
PRIMER_LEFT_32_TM=59.333
PRIMER_RIGHT_32_TM=61.655
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=402
PRIMER_PAIR_33_PENALTY=2.322029
PRIMER_LEFT_33_PENALTY=0.667126
PRIMER_RIGHT_33_PENALTY=1.654904
PRIMER_LEFT_33_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_33_SEQUENCE=TACTCCGTCACCGTTCTCCA
PRIMER_LEFT_33=892,20
PRIMER_RIGHT_33=1294,20
PRIMER_LEFT_33_TM=59.333
PRIMER_RIGHT_33_TM=61.655
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=403
PRIMER_PAIR_34_PENALTY=2.364831
PRIMER_LEFT_34_PENALTY=0.667126
PRIMER_RIGHT_34_PENALTY=1.697705
PRIMER_LEFT_34_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_34_SEQUENCE=AACGTCATAACCTTGATCCCA
PRIMER_LEFT_34=893,20
PRIMER_RIGHT_34=1546,21
PRIMER_LEFT_34_TM=59.333
PRIMER_RIGHT_34_TM=59.302
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.6000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=654
PRIMER_PAIR_35_PENALTY=2.364831
PRIMER_LEFT_35_PENALTY=0.667126
PRIMER_RIGHT_35_PENALTY=1.697705
PRIMER_LEFT_35_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_35_SEQUENCE=AACGTCATAACCTTGATCCCA
PRIMER_LEFT_35=892,20
PRIMER_RIGHT_35=1546,21
PRIMER_LEFT_35_TM=59.333
PRIMER_RIGHT_35_TM=59.302
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=655
PRIMER_PAIR_36_PENALTY=2.369007
PRIMER_LEFT_36_PENALTY=0.667126
PRIMER_RIGHT_36_PENALTY=1.701882
PRIMER_LEFT_36_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_36_SEQUENCE=AATCAACGAAGAAGCCATCA
PRIMER_LEFT_36=893,20
PRIMER_RIGHT_36=1649,20
PRIMER_LEFT_36_TM=59.333
PRIMER_RIGHT_36_TM=58.298
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=2.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=757
PRIMER_PAIR_37_PENALTY=2.369007
PRIMER_LEFT_37_PENALTY=0.667126
PRIMER_RIGHT_37_PENALTY=1.701882
PRIMER_LEFT_37_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_37_SEQUENCE=AATCAACGAAGAAGCCATCA
PRIMER_LEFT_37=892,20
PRIMER_RIGHT_37=1649,20
PRIMER_LEFT_37_TM=59.333
PRIMER_RIGHT_37_TM=58.298
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=2.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=758
PRIMER_PAIR_38_PENALTY=2.393439
PRIMER_LEFT_38_PENALTY=0.667126
PRIMER_RIGHT_38_PENALTY=1.726313
PRIMER_LEFT_38_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_38_SEQUENCE=CTTGATCCCACCTATCACCAC
PRIMER_LEFT_38=893,20
PRIMER_RIGHT_38=1535,21
PRIMER_LEFT_38_TM=59.333
PRIMER_RIGHT_38_TM=59.274
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=643
PRIMER_PAIR_39_PENALTY=2.393439
PRIMER_LEFT_39_PENALTY=0.667126
PRIMER_RIGHT_39_PENALTY=1.726313
PRIMER_LEFT_39_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_39_SEQUENCE=CTTGATCCCACCTATCACCAC
PRIMER_LEFT_39=892,20
PRIMER_RIGHT_39=1535,21
PRIMER_LEFT_39_TM=59.333
PRIMER_RIGHT_39_TM=59.274
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=644
PRIMER_PAIR_40_PENALTY=2.438723
PRIMER_LEFT_40_PENALTY=0.667126
PRIMER_RIGHT_40_PENALTY=1.771597
PRIMER_LEFT_40_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_40_SEQUENCE=CAATGACTTGGAGCGTAACC
PRIMER_LEFT_40=893,20
PRIMER_RIGHT_40=1500,20
PRIMER_LEFT_40_TM=59.333
PRIMER_RIGHT_40_TM=58.228
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=608
PRIMER_PAIR_41_PENALTY=2.438723
PRIMER_LEFT_41_PENALTY=0.667126
PRIMER_RIGHT_41_PENALTY=1.771597
PRIMER_LEFT_41_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_41_SEQUENCE=CAATGACTTGGAGCGTAACC
PRIMER_LEFT_41=892,20
PRIMER_RIGHT_41=1500,20
PRIMER_LEFT_41_TM=59.333
PRIMER_RIGHT_41_TM=58.228
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=7.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=609
PRIMER_PAIR_42_PENALTY=2.455240
PRIMER_LEFT_42_PENALTY=2.176393
PRIMER_RIGHT_42_PENALTY=0.278847
PRIMER_LEFT_42_SEQUENCE=GACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_42_SEQUENCE=CTACTCCGTCACCGTTCTCC
PRIMER_LEFT_42=892,21
PRIMER_RIGHT_42=1295,20
PRIMER_LEFT_42_TM=61.176
PRIMER_RIGHT_42_TM=59.721
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=60.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=404
PRIMER_PAIR_43_PENALTY=2.465968
PRIMER_LEFT_43_PENALTY=0.667126
PRIMER_RIGHT_43_PENALTY=1.798843
PRIMER_LEFT_43_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_43_SEQUENCE=TTGATCCCACCTATCACCAC
PRIMER_LEFT_43=893,20
PRIMER_RIGHT_43=1534,20
PRIMER_LEFT_43_TM=59.333
PRIMER_RIGHT_43_TM=58.201
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=642
PRIMER_PAIR_44_PENALTY=2.465968
PRIMER_LEFT_44_PENALTY=0.667126
PRIMER_RIGHT_44_PENALTY=1.798843
PRIMER_LEFT_44_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_44_SEQUENCE=TTGATCCCACCTATCACCAC
PRIMER_LEFT_44=892,20
PRIMER_RIGHT_44=1534,20
PRIMER_LEFT_44_TM=59.333
PRIMER_RIGHT_44_TM=58.201
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=643
PRIMER_PAIR_45_PENALTY=2.478910
PRIMER_LEFT_45_PENALTY=0.667126
PRIMER_RIGHT_45_PENALTY=1.811785
PRIMER_LEFT_45_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_45_SEQUENCE=AATCAACGAAGAAGCCATCAC
PRIMER_LEFT_45=893,20
PRIMER_RIGHT_45=1649,21
PRIMER_LEFT_45_TM=59.333
PRIMER_RIGHT_45_TM=59.188
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=6.3000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=757
PRIMER_PAIR_46_PENALTY=2.478910
PRIMER_LEFT_46_PENALTY=0.667126
PRIMER_RIGHT_46_PENALTY=1.811785
PRIMER_LEFT_46_SEQUENCE=GACACTGCCGAAGAAAAGGT
PRIMER_RIGHT_46_SEQUENCE=AATCAACGAAGAAGCCATCAC
PRIMER_LEFT_46=892,20
PRIMER_RIGHT_46=1649,21
PRIMER_LEFT_46_TM=59.333
PRIMER_RIGHT_46_TM=59.188
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.3000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=758
PRIMER_PAIR_47_PENALTY=2.515623
PRIMER_LEFT_47_PENALTY=2.176393
PRIMER_RIGHT_47_PENALTY=0.339231
PRIMER_LEFT_47_SEQUENCE=GACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_47_SEQUENCE=ATCCCACCTATCACCACGAC
PRIMER_LEFT_47=892,21
PRIMER_RIGHT_47=1531,20
PRIMER_LEFT_47_TM=61.176
PRIMER_RIGHT_47_TM=59.661
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=7.8000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=640
PRIMER_PAIR_48_PENALTY=2.719089
PRIMER_LEFT_48_PENALTY=2.176393
PRIMER_RIGHT_48_PENALTY=0.542697
PRIMER_LEFT_48_SEQUENCE=GACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_48_SEQUENCE=AGCAAATCAACGAAGAAGCC
PRIMER_LEFT_48=892,21
PRIMER_RIGHT_48=1653,20
PRIMER_LEFT_48_TM=61.176
PRIMER_RIGHT_48_TM=59.457
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=762
PRIMER_PAIR_49_PENALTY=2.783708
PRIMER_LEFT_49_PENALTY=0.667126
PRIMER_RIGHT_49_PENALTY=2.116582
PRIMER_LEFT_49_SEQUENCE=ACACTGCCGAAGAAAAGGTC
PRIMER_RIGHT_49_SEQUENCE=AACGTCATAACCTTGATCCCAC
PRIMER_LEFT_49=893,20
PRIMER_RIGHT_49=1546,22
PRIMER_LEFT_49_TM=59.333
PRIMER_RIGHT_49_TM=60.117
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=654
=
SEQUENCE_ID=MAGIC.149_3_14026382_14026769_rsch-4_kn-0_35185_12584_TRUE
SEQUENCE_TARGET=950,487
SEQUENCE_TEMPLATE=TGAGAAATCCCTGTCCGTGACCGAGAATCATTGAAGCTAACCTTAAACCTTCNTCNGAATCTCTGTGAGTCTCNAGCGTGTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTNTGNAACATCAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTGTCGAGTTTCAAAGTGACCGTCCGACCGAGAAATCGTGAAAAGTGTTACNATCGAGAAACTATGCCCGTGATCAAGATCCTTTNTNNGTANCTTATTATTTCCTGTCCGTGATNAAGANAACCTGTTTGTGGATGAGAAATCCCTGNCCGTGACCGAGAATCATTGAAGCTAACCTTAAACCTTNATCNGAATCTCTGTGAGTCTCNAGCGTGTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_1_16485339_16485351_bur-0_can-0_78_2564042_TRUE
SEQUENCE_TARGET=950,112
SEQUENCE_TEMPLATE=AAAAAAAAAATCAATGGAAGGAGGAGGNAGAGNACCAAATCAAACGATTCTCAGTGAAATAGAACATATGCCTGAAGCTCCACGTCAACGTATCTCTCATCACCGTCGAGCTCGCTCTGAAACCTTCTTCTCCGGCGAATCAATCGACGATCTCCTCTTATTCGATCCTTCCGANATCGATTTCTCTTCTCTAGACTTCCTCAACGCTCCACCACCACCACNACAATCACAACAACAACCNCAAGCTTCTCCCATGTCCGTTGATTCNGAAGAAACCTCATCNAACGGTGTTGTTCCTCCNAANNCTCTTCCTCCNAAACCCGAAGCTAGATTCGGTCGCCATGTTCGTAGCTTCTCGGTTGATTNCGATTTCTTCGATGATTTGGGTGTTACTGAGGAGAAGTNTNTAGCTACAAGTTCAGGAGAGAAGAAGAAAGGGNATCATCATCATAGCAGGAGTAATTCTATGGATGGAGAGATGAGTTCGGCGTCGTTTAATATCGAATCGATTTTAGCTTCTGTGAGTGGTAAAGATAGTGGGAAGAAGAATATGGGTATGGGTGGTGATAGACTTGCTGAGCTTGCTTTGCTTGATCCTAAAAGAGCTAAAAGGTATNTAATTTAGTGTTGNNNTTTACTTAAANNTTGTAACTTNTTTGNGATTTGATTGAGGTTTGAANNAGTGTTTTNNGNTGNNNTTTTGATAGGATTTTAGCGAATAGACAATCTGCGGCGAGGTCGAAAGAGAGGAAGATTAGGTATACTGGTGAGTTAGAGAGGAAGGTTCAGACACTTCAGAATGAAGCTACTACATTGTCTGCTCAAGTNACTATGTTACAGGTAATGTTTTNTATTACTTNGTCATNGTGTTTATGCAATTGGTGTGACTAGTGAAGGTTGGTGAGTANATTACNNNNNNTCTNNNNANCTNTNTNNNNNNNNNNNCTGATNTNNTNANTNNAGNTTNNNNNNNNGNACTNNNNNNNNTNNCTTGATTNGNNCNTGGCAGCTTNTANGCTTGTANTGNTGCTTAGTNTGTTTAAGATTGCTTCTTNGTGTNTAGAGAGGACATGTGTTTGCTACTAGNNNNNNNNATAGATGCAGNNTTATAAGATATNNTATTNTNATGTTAATTTAGCNNATNAATCAACATGTGAGATATGTCANTAGTGTAATGTATACANTTGCAAGAGTGTAANNTTTGTTTNCACTTTAGTGATAAAAGCAACATAGGAGTAAGATNAAATGATTCATGTNTCTTGCATCTCTAATCTGTGCTTTTTCTTATATTGTGTAGAGAGGAACATCAGAGCTGAACACTGAAAATAAACACCTCAAAATGCNGCTTCAAGCTTTAGAGCAACAAGCTGAACTTAGGGATGGTNAAGCCATTTTCTTNTTCATTGGNTCGTTCTCAATATGTANACCNAAGTCNNCNTTACGATATNTATTGTTCGGTGTAATTTTCAGCTTTGAATGAAGCNCTGCGGGATGAACTNAACCGACTTAAGGTGGTAGCTGGAGAAATTCCTCAGGGGAANGGAAATTCNTACAACCGTGCTCAATTCTCATCTCAGCAATCGGCAATGAATCAGTTTGGGAACAANANGAACCANCAGATGAGTACAAACGGGCAGCCATCGCTCCCAAGCTACATGGATTTCACCAAGAGAGGCTGAGTTCGTGTCACCTATATATGTTTGCTGAGTTATAAATACGTTATATTCATTTCGGGCTGCAATATTTGATGTATGTAAAAAGTATGTATGTATCCATGTATNATGGCTTTTGNNNTGTTANCGTCTATGCAGATATGTTCATGGCGANATCGGTCTATNTTTACNAGTAGTATCCATTGTAATATACTANCCAAAAATCTTTATAGTNAGCACTTTTCCTAGGCATTAAGCACCGATCATCAAACACGCAGCTAAATCNGNNGCTTAAGGCANNTACTTGCTGTTACGATAATGCTAAAACTNGGTAAACNATTGCGAANGACNAAGTGAAGG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.508608
PRIMER_LEFT_0_PENALTY=0.841762
PRIMER_RIGHT_0_PENALTY=0.666846
PRIMER_LEFT_0_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_0_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_0=733,20
PRIMER_RIGHT_0=1637,20
PRIMER_LEFT_0_TM=59.158
PRIMER_RIGHT_0_TM=60.667
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=905
PRIMER_PAIR_1_PENALTY=1.684734
PRIMER_LEFT_1_PENALTY=0.841762
PRIMER_RIGHT_1_PENALTY=0.842972
PRIMER_LEFT_1_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_1_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_1=733,20
PRIMER_RIGHT_1=1601,20
PRIMER_LEFT_1_TM=59.158
PRIMER_RIGHT_1_TM=60.843
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=869
PRIMER_PAIR_2_PENALTY=1.714406
PRIMER_LEFT_2_PENALTY=0.841762
PRIMER_RIGHT_2_PENALTY=0.872644
PRIMER_LEFT_2_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_2_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_2=733,20
PRIMER_RIGHT_2=1690,20
PRIMER_LEFT_2_TM=59.158
PRIMER_RIGHT_2_TM=60.873
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=958
PRIMER_PAIR_3_PENALTY=1.823411
PRIMER_LEFT_3_PENALTY=0.777580
PRIMER_RIGHT_3_PENALTY=1.045831
PRIMER_LEFT_3_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_3_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_3=578,20
PRIMER_RIGHT_3=1530,21
PRIMER_LEFT_3_TM=59.222
PRIMER_RIGHT_3_TM=59.954
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=953
PRIMER_PAIR_4_PENALTY=1.887594
PRIMER_LEFT_4_PENALTY=0.841762
PRIMER_RIGHT_4_PENALTY=1.045831
PRIMER_LEFT_4_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_4_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_4=733,20
PRIMER_RIGHT_4=1530,21
PRIMER_LEFT_4_TM=59.158
PRIMER_RIGHT_4_TM=59.954
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=798
PRIMER_PAIR_5_PENALTY=1.960235
PRIMER_LEFT_5_PENALTY=0.841762
PRIMER_RIGHT_5_PENALTY=1.118473
PRIMER_LEFT_5_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_5_SEQUENCE=TAGGTGACACGAACTCAGCC
PRIMER_LEFT_5=733,20
PRIMER_RIGHT_5=1692,20
PRIMER_LEFT_5_TM=59.158
PRIMER_RIGHT_5_TM=58.882
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=960
PRIMER_PAIR_6_PENALTY=1.982603
PRIMER_LEFT_6_PENALTY=0.841762
PRIMER_RIGHT_6_PENALTY=1.140841
PRIMER_LEFT_6_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_6_SEQUENCE=CTGATTCATTGCCGATTGCT
PRIMER_LEFT_6=733,20
PRIMER_RIGHT_6=1594,20
PRIMER_LEFT_6_TM=59.158
PRIMER_RIGHT_6_TM=61.141
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=862
PRIMER_PAIR_7_PENALTY=2.053590
PRIMER_LEFT_7_PENALTY=0.777580
PRIMER_RIGHT_7_PENALTY=1.276010
PRIMER_LEFT_7_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_7_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_7=578,20
PRIMER_RIGHT_7=1571,20
PRIMER_LEFT_7_TM=59.222
PRIMER_RIGHT_7_TM=58.724
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.8000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=994
PRIMER_PAIR_8_PENALTY=2.117772
PRIMER_LEFT_8_PENALTY=0.841762
PRIMER_RIGHT_8_PENALTY=1.276010
PRIMER_LEFT_8_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_8_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_8=733,20
PRIMER_RIGHT_8=1571,20
PRIMER_LEFT_8_TM=59.158
PRIMER_RIGHT_8_TM=58.724
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=839
PRIMER_PAIR_9_PENALTY=2.181722
PRIMER_LEFT_9_PENALTY=0.095771
PRIMER_RIGHT_9_PENALTY=2.085951
PRIMER_LEFT_9_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_9_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_9=326,20
PRIMER_RIGHT_9=1321,22
PRIMER_LEFT_9_TM=60.096
PRIMER_RIGHT_9_TM=59.914
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=996
PRIMER_PAIR_10_PENALTY=2.340266
PRIMER_LEFT_10_PENALTY=1.938224
PRIMER_RIGHT_10_PENALTY=0.402042
PRIMER_LEFT_10_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_10_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_10=732,20
PRIMER_RIGHT_10=1683,20
PRIMER_LEFT_10_TM=61.938
PRIMER_RIGHT_10_TM=59.598
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=8.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=952
PRIMER_PAIR_11_PENALTY=2.394411
PRIMER_LEFT_11_PENALTY=1.348580
PRIMER_RIGHT_11_PENALTY=1.045831
PRIMER_LEFT_11_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_11_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_11=572,20
PRIMER_RIGHT_11=1530,21
PRIMER_LEFT_11_TM=61.349
PRIMER_RIGHT_11_TM=59.954
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=959
PRIMER_PAIR_12_PENALTY=2.423693
PRIMER_LEFT_12_PENALTY=1.377862
PRIMER_RIGHT_12_PENALTY=1.045831
PRIMER_LEFT_12_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_12_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_12=579,21
PRIMER_RIGHT_12=1530,21
PRIMER_LEFT_12_TM=59.622
PRIMER_RIGHT_12_TM=59.954
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.5000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=952
PRIMER_PAIR_13_PENALTY=2.429008
PRIMER_LEFT_13_PENALTY=0.777580
PRIMER_RIGHT_13_PENALTY=1.651428
PRIMER_LEFT_13_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_13_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_13=578,20
PRIMER_RIGHT_13=1572,21
PRIMER_LEFT_13_TM=59.222
PRIMER_RIGHT_13_TM=60.651
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.8000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=995
PRIMER_PAIR_14_PENALTY=2.454032
PRIMER_LEFT_14_PENALTY=0.841762
PRIMER_RIGHT_14_PENALTY=1.612269
PRIMER_LEFT_14_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_14_SEQUENCE=GAGATGAGAATTGAGCACGG
PRIMER_LEFT_14=733,20
PRIMER_RIGHT_14=1574,20
PRIMER_LEFT_14_TM=59.158
PRIMER_RIGHT_14_TM=58.388
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=842
PRIMER_PAIR_15_PENALTY=2.468002
PRIMER_LEFT_15_PENALTY=2.065960
PRIMER_RIGHT_15_PENALTY=0.402042
PRIMER_LEFT_15_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_15_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_15=879,22
PRIMER_RIGHT_15=1683,20
PRIMER_LEFT_15_TM=60.066
PRIMER_RIGHT_15_TM=59.598
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=805
PRIMER_PAIR_16_PENALTY=2.468002
PRIMER_LEFT_16_PENALTY=2.065960
PRIMER_RIGHT_16_PENALTY=0.402042
PRIMER_LEFT_16_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_16_SEQUENCE=ACGAACTCAGCCTCTCTTGG
PRIMER_LEFT_16=879,22
PRIMER_RIGHT_16=1684,20
PRIMER_LEFT_16_TM=60.066
PRIMER_RIGHT_16_TM=59.598
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=806
PRIMER_PAIR_17_PENALTY=2.474358
PRIMER_LEFT_17_PENALTY=0.388407
PRIMER_RIGHT_17_PENALTY=2.085951
PRIMER_LEFT_17_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_17_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_17=468,20
PRIMER_RIGHT_17=1321,22
PRIMER_LEFT_17_TM=59.612
PRIMER_RIGHT_17_TM=59.914
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=10.2000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=854
PRIMER_PAIR_18_PENALTY=2.489553
PRIMER_LEFT_18_PENALTY=2.065960
PRIMER_RIGHT_18_PENALTY=0.423592
PRIMER_LEFT_18_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_18_SEQUENCE=ATATTGCAGCCCGAAATGAA
PRIMER_LEFT_18=879,22
PRIMER_RIGHT_18=1743,20
PRIMER_LEFT_18_TM=60.066
PRIMER_RIGHT_18_TM=60.424
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=865
PRIMER_PAIR_19_PENALTY=2.489553
PRIMER_LEFT_19_PENALTY=2.065960
PRIMER_RIGHT_19_PENALTY=0.423592
PRIMER_LEFT_19_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_19_SEQUENCE=ATCAAATATTGCAGCCCGAA
PRIMER_LEFT_19=879,22
PRIMER_RIGHT_19=1748,20
PRIMER_LEFT_19_TM=60.066
PRIMER_RIGHT_19_TM=60.424
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=10.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=870
PRIMER_PAIR_20_PENALTY=2.493191
PRIMER_LEFT_20_PENALTY=0.841762
PRIMER_RIGHT_20_PENALTY=1.651428
PRIMER_LEFT_20_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_20_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_20=733,20
PRIMER_RIGHT_20=1572,21
PRIMER_LEFT_20_TM=59.158
PRIMER_RIGHT_20_TM=60.651
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=840
PRIMER_PAIR_21_PENALTY=2.544250
PRIMER_LEFT_21_PENALTY=0.841762
PRIMER_RIGHT_21_PENALTY=1.702488
PRIMER_LEFT_21_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_21_SEQUENCE=ATTGCCGATTGCTGAGATGA
PRIMER_LEFT_21=733,20
PRIMER_RIGHT_21=1587,20
PRIMER_LEFT_21_TM=59.158
PRIMER_RIGHT_21_TM=61.702
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=855
PRIMER_PAIR_22_PENALTY=2.571769
PRIMER_LEFT_22_PENALTY=0.841762
PRIMER_RIGHT_22_PENALTY=1.730006
PRIMER_LEFT_22_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_22_SEQUENCE=CATGTAGCTTGGGAGCGATG
PRIMER_LEFT_22=733,20
PRIMER_RIGHT_22=1657,20
PRIMER_LEFT_22_TM=59.158
PRIMER_RIGHT_22_TM=61.730
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=925
PRIMER_PAIR_23_PENALTY=2.605069
PRIMER_LEFT_23_PENALTY=1.938224
PRIMER_RIGHT_23_PENALTY=0.666846
PRIMER_LEFT_23_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_23_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_23=732,20
PRIMER_RIGHT_23=1637,20
PRIMER_LEFT_23_TM=61.938
PRIMER_RIGHT_23_TM=60.667
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=906
PRIMER_PAIR_24_PENALTY=2.615615
PRIMER_LEFT_24_PENALTY=0.841762
PRIMER_RIGHT_24_PENALTY=1.773852
PRIMER_LEFT_24_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_24_SEQUENCE=ATAGGTGACACGAACTCAGCC
PRIMER_LEFT_24=733,20
PRIMER_RIGHT_24=1693,21
PRIMER_LEFT_24_TM=59.158
PRIMER_RIGHT_24_TM=59.226
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=9.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=961
PRIMER_PAIR_25_PENALTY=2.624590
PRIMER_LEFT_25_PENALTY=1.348580
PRIMER_RIGHT_25_PENALTY=1.276010
PRIMER_LEFT_25_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_25_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_25=572,20
PRIMER_RIGHT_25=1571,20
PRIMER_LEFT_25_TM=61.349
PRIMER_RIGHT_25_TM=58.724
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=1000
PRIMER_PAIR_26_PENALTY=2.653872
PRIMER_LEFT_26_PENALTY=1.377862
PRIMER_RIGHT_26_PENALTY=1.276010
PRIMER_LEFT_26_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_26_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_26=579,21
PRIMER_RIGHT_26=1571,20
PRIMER_LEFT_26_TM=59.622
PRIMER_RIGHT_26_TM=58.724
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.5000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=993
PRIMER_PAIR_27_PENALTY=2.678904
PRIMER_LEFT_27_PENALTY=2.012058
PRIMER_RIGHT_27_PENALTY=0.666846
PRIMER_LEFT_27_SEQUENCE=CGAGGTCGAAAGAGAGGAAGA
PRIMER_RIGHT_27_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_27=733,21
PRIMER_RIGHT_27=1637,20
PRIMER_LEFT_27_TM=61.012
PRIMER_RIGHT_27_TM=60.667
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=905
PRIMER_PAIR_28_PENALTY=2.683451
PRIMER_LEFT_28_PENALTY=0.841762
PRIMER_RIGHT_28_PENALTY=1.841689
PRIMER_LEFT_28_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_28_SEQUENCE=CTCAGCCTCTCTTGGTGAAA
PRIMER_LEFT_28=733,20
PRIMER_RIGHT_28=1679,20
PRIMER_LEFT_28_TM=59.158
PRIMER_RIGHT_28_TM=58.158
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=8.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=947
PRIMER_PAIR_29_PENALTY=2.724911
PRIMER_LEFT_29_PENALTY=0.388407
PRIMER_RIGHT_29_PENALTY=2.336505
PRIMER_LEFT_29_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_29_SEQUENCE=CCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_29=468,20
PRIMER_RIGHT_29=1378,21
PRIMER_LEFT_29_TM=59.612
PRIMER_RIGHT_29_TM=58.663
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=10.2000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=911
PRIMER_PAIR_30_PENALTY=2.732806
PRIMER_LEFT_30_PENALTY=2.065960
PRIMER_RIGHT_30_PENALTY=0.666846
PRIMER_LEFT_30_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_30_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_30=879,22
PRIMER_RIGHT_30=1637,20
PRIMER_LEFT_30_TM=60.066
PRIMER_RIGHT_30_TM=60.667
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=759
PRIMER_PAIR_31_PENALTY=2.735536
PRIMER_LEFT_31_PENALTY=0.388407
PRIMER_RIGHT_31_PENALTY=2.347129
PRIMER_LEFT_31_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_31_SEQUENCE=CCATCCCTAAGTTCAGCTTGTT
PRIMER_LEFT_31=468,20
PRIMER_RIGHT_31=1382,22
PRIMER_LEFT_31_TM=59.612
PRIMER_RIGHT_31_TM=59.653
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=10.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=915
PRIMER_PAIR_32_PENALTY=2.765798
PRIMER_LEFT_32_PENALTY=0.841762
PRIMER_RIGHT_32_PENALTY=1.924036
PRIMER_LEFT_32_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_32_SEQUENCE=ACTCAGCCTCTCTTGGTGAAA
PRIMER_LEFT_32=733,20
PRIMER_RIGHT_32=1680,21
PRIMER_LEFT_32_TM=59.158
PRIMER_RIGHT_32_TM=59.076
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=8.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=948
PRIMER_PAIR_33_PENALTY=2.781195
PRIMER_LEFT_33_PENALTY=1.938224
PRIMER_RIGHT_33_PENALTY=0.842972
PRIMER_LEFT_33_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_33_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_33=732,20
PRIMER_RIGHT_33=1601,20
PRIMER_LEFT_33_TM=61.938
PRIMER_RIGHT_33_TM=60.843
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=870
PRIMER_PAIR_34_PENALTY=2.788857
PRIMER_LEFT_34_PENALTY=0.841762
PRIMER_RIGHT_34_PENALTY=1.947095
PRIMER_LEFT_34_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_34_SEQUENCE=CCAAACTGATTCATTGCCGA
PRIMER_LEFT_34=733,20
PRIMER_RIGHT_34=1599,20
PRIMER_LEFT_34_TM=59.158
PRIMER_RIGHT_34_TM=61.947
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=11.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=867
PRIMER_PAIR_35_PENALTY=2.810867
PRIMER_LEFT_35_PENALTY=1.938224
PRIMER_RIGHT_35_PENALTY=0.872644
PRIMER_LEFT_35_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_35_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_35=732,20
PRIMER_RIGHT_35=1690,20
PRIMER_LEFT_35_TM=61.938
PRIMER_RIGHT_35_TM=60.873
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=60.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=959
PRIMER_PAIR_36_PENALTY=2.812798
PRIMER_LEFT_36_PENALTY=0.388407
PRIMER_RIGHT_36_PENALTY=2.424392
PRIMER_LEFT_36_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_36_SEQUENCE=CCCTAAGTTCAGCTTGTTGCTC
PRIMER_LEFT_36=468,20
PRIMER_RIGHT_36=1378,22
PRIMER_LEFT_36_TM=59.612
PRIMER_RIGHT_36_TM=60.424
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.2000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=911
PRIMER_PAIR_37_PENALTY=2.812798
PRIMER_LEFT_37_PENALTY=0.388407
PRIMER_RIGHT_37_PENALTY=2.424392
PRIMER_LEFT_37_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_37_SEQUENCE=TCCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_37=468,20
PRIMER_RIGHT_37=1379,22
PRIMER_LEFT_37_TM=59.612
PRIMER_RIGHT_37_TM=60.424
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.2000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=912
PRIMER_PAIR_38_PENALTY=2.855030
PRIMER_LEFT_38_PENALTY=2.012058
PRIMER_RIGHT_38_PENALTY=0.842972
PRIMER_LEFT_38_SEQUENCE=CGAGGTCGAAAGAGAGGAAGA
PRIMER_RIGHT_38_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_38=733,21
PRIMER_RIGHT_38=1601,20
PRIMER_LEFT_38_TM=61.012
PRIMER_RIGHT_38_TM=60.843
PRIMER_LEFT_38_GC_PERCENT=52.381
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=10.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=869
PRIMER_PAIR_39_PENALTY=2.863531
PRIMER_LEFT_39_PENALTY=0.777580
PRIMER_RIGHT_39_PENALTY=2.085951
PRIMER_LEFT_39_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_39_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_39=578,20
PRIMER_RIGHT_39=1321,22
PRIMER_LEFT_39_TM=59.222
PRIMER_RIGHT_39_TM=59.914
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.8000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=744
PRIMER_PAIR_40_PENALTY=2.872108
PRIMER_LEFT_40_PENALTY=0.786156
PRIMER_RIGHT_40_PENALTY=2.085951
PRIMER_LEFT_40_SEQUENCE=CCATGTTCGTAGCTTCTCGG
PRIMER_RIGHT_40_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_40=339,20
PRIMER_RIGHT_40=1321,22
PRIMER_LEFT_40_TM=60.786
PRIMER_RIGHT_40_TM=59.914
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=983
PRIMER_PAIR_41_PENALTY=2.908932
PRIMER_LEFT_41_PENALTY=2.065960
PRIMER_RIGHT_41_PENALTY=0.842972
PRIMER_LEFT_41_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_41_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_41=879,22
PRIMER_RIGHT_41=1601,20
PRIMER_LEFT_41_TM=60.066
PRIMER_RIGHT_41_TM=60.843
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=10.0000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=723
PRIMER_PAIR_42_PENALTY=2.919343
PRIMER_LEFT_42_PENALTY=0.841762
PRIMER_RIGHT_42_PENALTY=2.077581
PRIMER_LEFT_42_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_42_SEQUENCE=GATTCATTGCCGATTGCTGA
PRIMER_LEFT_42=733,20
PRIMER_RIGHT_42=1592,20
PRIMER_LEFT_42_TM=59.158
PRIMER_RIGHT_42_TM=62.078
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=860
PRIMER_PAIR_43_PENALTY=2.927714
PRIMER_LEFT_43_PENALTY=0.841762
PRIMER_RIGHT_43_PENALTY=2.085951
PRIMER_LEFT_43_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_43_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_43=733,20
PRIMER_RIGHT_43=1321,22
PRIMER_LEFT_43_TM=59.158
PRIMER_RIGHT_43_TM=59.914
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=589
PRIMER_PAIR_44_PENALTY=2.937401
PRIMER_LEFT_44_PENALTY=2.065960
PRIMER_RIGHT_44_PENALTY=0.871441
PRIMER_LEFT_44_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_44_SEQUENCE=TGACACGAACTCAGCCTCTC
PRIMER_LEFT_44=879,22
PRIMER_RIGHT_44=1688,20
PRIMER_LEFT_44_TM=60.066
PRIMER_RIGHT_44_TM=59.129
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=810
PRIMER_PAIR_45_PENALTY=2.938604
PRIMER_LEFT_45_PENALTY=2.065960
PRIMER_RIGHT_45_PENALTY=0.872644
PRIMER_LEFT_45_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_45_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_45=879,22
PRIMER_RIGHT_45=1690,20
PRIMER_LEFT_45_TM=60.066
PRIMER_RIGHT_45_TM=60.873
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=60.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=812
PRIMER_PAIR_46_PENALTY=2.947204
PRIMER_LEFT_46_PENALTY=1.901373
PRIMER_RIGHT_46_PENALTY=1.045831
PRIMER_LEFT_46_SEQUENCE=GCTGAGCTTGCTTTGCTTGA
PRIMER_RIGHT_46_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_46=574,20
PRIMER_RIGHT_46=1530,21
PRIMER_LEFT_46_TM=61.901
PRIMER_RIGHT_46_TM=59.954
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=957
PRIMER_PAIR_47_PENALTY=2.976022
PRIMER_LEFT_47_PENALTY=2.573980
PRIMER_RIGHT_47_PENALTY=0.402042
PRIMER_LEFT_47_SEQUENCE=ATAGACAATCTGCGGCGAGG
PRIMER_RIGHT_47_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_47=718,20
PRIMER_RIGHT_47=1683,20
PRIMER_LEFT_47_TM=62.574
PRIMER_RIGHT_47_TM=59.598
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=966
PRIMER_PAIR_48_PENALTY=2.976022
PRIMER_LEFT_48_PENALTY=2.573980
PRIMER_RIGHT_48_PENALTY=0.402042
PRIMER_LEFT_48_SEQUENCE=ATAGACAATCTGCGGCGAGG
PRIMER_RIGHT_48_SEQUENCE=ACGAACTCAGCCTCTCTTGG
PRIMER_LEFT_48=718,20
PRIMER_RIGHT_48=1684,20
PRIMER_LEFT_48_TM=62.574
PRIMER_RIGHT_48_TM=59.598
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.9000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=967
PRIMER_PAIR_49_PENALTY=2.984055
PRIMER_LEFT_49_PENALTY=1.938224
PRIMER_RIGHT_49_PENALTY=1.045831
PRIMER_LEFT_49_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_49_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_49=732,20
PRIMER_RIGHT_49=1530,21
PRIMER_LEFT_49_TM=61.938
PRIMER_RIGHT_49_TM=59.954
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=799
=
SEQUENCE_ID=MAGIC.105_4_17500711_17501062_edi-0_bur-0_27220_9632_TRUE
SEQUENCE_TARGET=950,451
SEQUENCE_TEMPLATE=TTCACAAGAACCTACCAACTCGGAAAACAAAGAAAACCCTTCCCGCCATCTACTANACTTGAAATATCTATCTATCATTGATGTCCACGAGACAACATCTTTCTCAACAATCTTATCAAATATGTTTCTTGCTTCATCGATACAGCCACATTTTCCATACATATCCATCAATGAACTCCAANGCACTTCNTCNGAATCCAAACCAGCTCGAACGATATGCCCATGAATCTCTTTCCCTCTACGGATACATTTAACCGCAGCAGCAGCAGCTACTGCAATAGAAACTGTAAAAATGTTTGGTCTTGAATTCGGTACCCGTTGCATCAAACTATACAACACCAATGCCTCTTCAGGCTGATCCTTCTTAACATAACCCGTAACCATAGCCGTCCACGAATAACTATCTTTTTCAGTCATTTCATCAAACAACTTCCTCGCTTCCTCAAGCAACCCAACCTCCGCATACCCATTAACCATTACATTCCACGAACACAAATCTCTATTCGGCATTTCGTCGAACACCTTCCGTGCATCAACCAAGCTACCACATTTCGCATACATTCTCAAAAGACGGTTCCATATAACAATCCCAGGTACAAACCCANAAGTCCTAATGTGTTCATGGACCTTCTTACCTTCTTCAAGAGCACGTGTTTGGCTACAAACTTGAATGAGATTGCAATAAGTAGAAGCAGGTGGCTTCTTNGCNCGACCCAGAAGTTNAACAGCTTCTCTTAAGAGCTTCTGTCCACATAATACATCAATGGCTTCACCAAATCTATTAGCTNTACATAATCGNTCCACCACAACACCACCGTCCTCATGGTTCGNATTGAAGAACTTTTTATCTGAAAACCGCTTNNGTGAGGCGTGANAAGATGATAGATTTCTCTTNAATATAAATCCATGNANAGTAGTTAACTTTCTCGCATTTGAATGATTCATCTTCTTCACCAAAACAAAAAGAGTTNAAATTCAAAGATCTCGTCNGCGGCAACATNAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAANCAAAAGTAAAAANAAAAAAGCTCCGATGCCGGGAATCGAACCCGGGTCTCCTGGGTGAAAGCCAGATATCCTAACCGCTGGACGACATCGGATTTGTTGTTNTATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_8929743_8930028_wil-2_sf-2_315701_189930_TRUE
SEQUENCE_TARGET=950,385
SEQUENCE_TEMPLATE=CAAGTAATGAACAATGAAAGCTTNAGATNNNNNNNACTCANAANCTCATCTGCTACCAAATCATTACCTANATCAAACTGATAAGTATGATCTACACAGGCACAGNACANNNNNNNNNNNNNNANANTTTNAGATTCCAAATACAANNTCTCAAGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANAGTAGCAATGTGAAATNTCGANGAATCTNNNNNNNNNNNNNNNNNAGGANTCGANNNNNNNAGAGANACTAACNATAGGGAAATCGAGAGGNTTGCGACGAGTGAGCTTCTCTTCNTCAGGAGCCATAGCCACCACCTGTAACTGCTTCCTTGTCTTGCGGCTTAGAGCGAAGCGTTGAACGGNGANGAAAGAGCANGANAAGCCATGGTTGATTCGANAGAAAGGGGAANNANTAGACGNAGCAGNTCTGTTTANCGACGNTGAAGATAGTGGCGGAGAGAGAAGTGGCCACGGNGGCGAAGGNGATGCCGATATNGAAAGCGGCGTCATAGCTCCGCCTCGTGTTCTTCGANGGNTNCTATCTGTTCCGTCNAACAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAATAGTTCNTGATGNTATNTGATNTTTNNNNNNNNNNNNNNNNNNNATGTATNNATTCTCAAATCTTTGACCANTACCAATNACATNACTNGANTCGCCAACNTNTATATNTANNGAAGAANGAATATATGGCTAAGNNNNNNNTACNAAGTACTACGNCNNNNNNGAAATAANCATTCANAAGTCTAAATTANTTTTGCTCNNNNNNNNAAAANTGATGAACGTTTCTCTCAAACTNTCATTNNTGGNTTTNANTTTGGTCATCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNCAAAAATATTTTNTATTTGNTNAGNTAAAANAAATTCAGAACATTTAANNNNTNAGTNTACTAANANAGTGATGAATGANTAGATCTTGGATCAGAAGCAAGNNAACTAGCCGNNGTTNACGAGANATTCGAAATTGCTGCGNGAAGNTCAAACNATNCCGGNACCGCTAGGNCCTTGCAGCAAGCCCCACCTTGCAAGAGGGATGTTGATTGTAGCTTTGAATGTCCCAAGGGNGGATTCTGCAATNATCGNCTTGGCACNTNTGANTGTTTCTAATTGTATTATTTGANTTCTAATCTACTTTTATCAATAAAGTTTATCCANCAATTNTTAAGACTTGATACTANCGAGGAAATNTTTTCNAAAGATNGGTTGTGATTACCNTTGGTGAACCTATAATCTTNTTCAACAAACTATAGAATNTTGACTCTNNNNNNNNNNNNNNNNNNNNAATCTCCNCACNTTTATNTGCATAGTNTNTCTCNNTGAAAGGCAAANCTNGCAGNNNNNNCNGAGGAATGNATGGTGAANCCAAGATCTNGTTTACNAAACCANNNNNNNTTNNNNNNNNCTTNCAAGTTGATCCCAATAACTCTTCCATCAATNTCAACCANNTTNCTCACCAAGATTATACATANAAATGGTCTGAAANCACTGGCGGNCATTCTCTTTTCCAAATATGCGGTCNACANAANTCANAACTCACTANNTAGNTNATAAGNCTGCAAAGTTTTTATGTCGAAANTTANAANNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_14632731_14632777_rsch-4_oy-0_70230_9323_TRUE
SEQUENCE_TARGET=950,146
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4328046_4328288_wil-2_ws-0_11541_46750_TRUE
SEQUENCE_TARGET=950,342
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4512569_4513290_kn-0_po-0_20239_14288_TRUE
SEQUENCE_TARGET=950,821
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCNCGGAAGGGAATTTGGGGAACCGAAGATAGTTCTTCGAACTCCNGAGGAATTGCCTGNATTCAAAGAATGAACGCGAGATTGAATAGTAATAACATTTTGACTGAGTGATATCTGGGTTNTTTGCAGTATTTTGACGGTTNTTTCCACATTCGAAAACTTCTGCGCCATTTCNGCCATAGTTTGCTGCATGGTCTTGAGAGTTTCGGCTAGTGAAGCGTTGGTAGCATCTTCTCCATACATTGTNGCTCACTTCGGAAATGTCGTGTATAGNAAAAATCTGATGACTCAAGAATTCTTTGTCNCCACAGANGNCGCCAAAATGTGGATNTCGGATTCACACACTCAAGTATTTATTTANTTAGNGAGAAGGATTATTCTTGAGGACAAATGTACTAGAAANTAGATTGATGNTTATAGNNNNNNNNNNNNNNNNNNNNAGAAAATGTGTATAGTAAGTTTGCACAAAAATGTGTGTGTNTNTACATGGCCTTTGACCCACAATTTATANNCAAAGANTACATTTAATTCCCATTTACACACAATTATTCTANANTAACTATTCNAATGTTCAAATTTGAATTGATTCCGAACTTTCTGGGCTTCGTCCTNTGGCCCACCCGACTCTCCAATCCGGNCAGTTCTGACATCGATGTCCAGTCCACCTCGCTGATCAATNNNNNNNNNNNTACNNTTTTACTCAGAAAAGCCCATGGGCCTTTCCTCTTTTAGACCCTTCCCTCTTACGTGGCGATCTTTGACNAGNCCCGACCCCACGGTGCGGGATCCGACACCAACNNTNNCTATCATATTCCGNTCCTCGATGCACTTTAATAAGAACACTCTATAACTCGACTCTACNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_8156157_8156556_ws-0_can-0_375350_1762_TRUE
SEQUENCE_TARGET=950,499
SEQUENCE_TEMPLATE=CGACTCGTCAAGACGTTATCTCAGGAGAAAGANACAAAGGAAACNGTGNGAAACAGGAAAGNNAAACAANACAAAGCCTTTGTTNAACAGTGTACCACTGGGGCTCTTCCTGGAAGTNNTTGAAGGAAAAGCTAGGGTAAGATGAACANTNACCTTGAGGCTTATCCGGAGTGAATTNATAGATGTTTCTATTAAACACTTTTCAGCCTCATTTCGGCATATTAAAACCTGCAAGTTGNAAAACTTAGCAGTCAACTACAAAACCTAGGTAAGAAGACAATGCAATATCAACGCTAAACTGGGAGGGAAACAAGTAAACTTCTTACAGGATTTAGCAAAAGTTCTGGGCTCGTCCTACAAAATTTAGCAAACACATGGTGATAATAAGATTCTATGCTTGAAATAAAAATGGAAGAAGNGCACCCCATTCTCACATGCATCNTCTTGTGAGAAGCTTACATACTTTAATTCAACCTCTGGTTTATTATGCCTTTCAACTTCTTGACAAGGAAAATTCTGCAAGTAGAGAAGTATAGAAGCTTTCAGGAATATAAACCATGTAAANCTGAATGTTTTCAAAGACTCCACATGGCCCCTTTCACCTACTTTAACGAAATCNNTAGGTTCTGACACCAAATATTGNGAGAAAAANGTCAAAAAGTAAGTGAATGATGGTACANCATGTNTAATCAAAAAGTAAACAACTAATACANGAGAATAACAGACCAAAGGTGGATGAATGAGATAGAACGCNAACATGAAGCTTTAACTGCCTTAAACAAACTTAACCATAAAGATGGANACTTTGAGACCTATGNCATGATANCNTGAACCAAAAATAATTTGTACCTGTAAGCACATNGCNGCCTCGAGTGTATTTTTTATGCATGCTAGATACAGCCGTAATGAGTTTGCCTGGANCATCAAAGACCCATCNGAANCAAATTTNAAACAATNTCTGCAGTAAATCAGTATGCGANAGACNTTGTTNNAAANAANNNTTTCTAAACNGGACATGTTTGAATGAAAAANAGTCTTTGTCAAGTATGAGAAGAATCAAAATTNGAATTACAGTCAANGTTCTTACCATGTCCTAGAAATGATGTTATTCTACTCNAGACTTGCACCAGACTTAGGATTTTATCTTTCTTNTCGATGAATCTTTATGNGCTGAAAAAATAATCCAAACATAAACAANNAAGCATCTTTAGTNTCATAGAATTTNGGTTACCAACTTTTTTANACTAAACNTCAANAAGAAAGCAACTTTAGGANCAAAATGTCTACAAGAAAACANNNNNNNNNNNNNNNNNNNNNNNNNGAAGAGAACAAAANAANAANNAAGTAACAGCAAGATCCCNGAGNCTAGAGTTTCTATGTAATCAACTCGAANCAACAANAAGNNNATGATTATTNAGAGACCCANTNANANAAATAAGCCAATCTACTGAAAGTCTGAAACTTTTGGAAAATGTGAGAAAGNGAAATCNAATTAGANNCCTAGGAATCAATCAGANANATCAAAAGNCCTAAAACCTTGNTCGTGANCNAATTGCATACNTTGGACGACTTGGAGACTCGTGAGAGGAGAGATCGNAAGGAGATTTAGAGATCAACGATCGGAAAATTGAATCACGATCAAATTGACCGGATAAGTGAAACGAAATGCGTCAAAACCGAACCAAACCAGACACNCCGACCGGATCTGGTACATTCTAAAATGGAATGTTCATTGACCCACAAATACCAGCNAGATTCTGCNGATTGAACCGGTGAACCGNGTTGCTCTACTTCTAAGTTCTAATCTACAACTACAAGNGGTACTTGCGCTTTAACACATCTGTTTATGTTANTNTATTNATTTATAGNAAAGACTGGCTCTGCTTCTTGTAGAATGTTCTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANCAACAGAGCAACAACTGTANGCAAAACNAAACAAAAAGNAANANNNNNNGNCNNNNNNNANNNNGTCTAATATCTACTATATGAGTATGACATTCCCATTTGGTTGTATATTGCATAGGTTCGG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.158190
PRIMER_LEFT_0_PENALTY=0.139753
PRIMER_RIGHT_0_PENALTY=0.018437
PRIMER_LEFT_0_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_0_SEQUENCE=GGTTTTGACGCATTTCGTTT
PRIMER_LEFT_0=896,20
PRIMER_RIGHT_0=1677,20
PRIMER_LEFT_0_TM=60.140
PRIMER_RIGHT_0_TM=59.982
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=782
PRIMER_PAIR_1_PENALTY=0.272322
PRIMER_LEFT_1_PENALTY=0.139753
PRIMER_RIGHT_1_PENALTY=0.132570
PRIMER_LEFT_1_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_1_SEQUENCE=GTGTCTGGTTTGGTTCGGTT
PRIMER_LEFT_1=896,20
PRIMER_RIGHT_1=1693,20
PRIMER_LEFT_1_TM=60.140
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=798
PRIMER_PAIR_2_PENALTY=0.288775
PRIMER_LEFT_2_PENALTY=0.270338
PRIMER_RIGHT_2_PENALTY=0.018437
PRIMER_LEFT_2_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_2_SEQUENCE=GGTTTTGACGCATTTCGTTT
PRIMER_LEFT_2=898,20
PRIMER_RIGHT_2=1677,20
PRIMER_LEFT_2_TM=60.270
PRIMER_RIGHT_2_TM=59.982
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=780
PRIMER_PAIR_3_PENALTY=0.288775
PRIMER_LEFT_3_PENALTY=0.270338
PRIMER_RIGHT_3_PENALTY=0.018437
PRIMER_LEFT_3_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_3_SEQUENCE=GGTTTTGACGCATTTCGTTT
PRIMER_LEFT_3=897,20
PRIMER_RIGHT_3=1677,20
PRIMER_LEFT_3_TM=60.270
PRIMER_RIGHT_3_TM=59.982
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=781
PRIMER_PAIR_4_PENALTY=0.402907
PRIMER_LEFT_4_PENALTY=0.270338
PRIMER_RIGHT_4_PENALTY=0.132570
PRIMER_LEFT_4_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_4_SEQUENCE=GTGTCTGGTTTGGTTCGGTT
PRIMER_LEFT_4=898,20
PRIMER_RIGHT_4=1693,20
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=796
PRIMER_PAIR_5_PENALTY=0.402907
PRIMER_LEFT_5_PENALTY=0.270338
PRIMER_RIGHT_5_PENALTY=0.132570
PRIMER_LEFT_5_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_5_SEQUENCE=GTGTCTGGTTTGGTTCGGTT
PRIMER_LEFT_5=897,20
PRIMER_RIGHT_5=1693,20
PRIMER_LEFT_5_TM=60.270
PRIMER_RIGHT_5_TM=59.867
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=797
PRIMER_PAIR_6_PENALTY=0.428042
PRIMER_LEFT_6_PENALTY=0.139753
PRIMER_RIGHT_6_PENALTY=0.288289
PRIMER_LEFT_6_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_6_SEQUENCE=TTTGACGCATTTCGTTTCAC
PRIMER_LEFT_6=896,20
PRIMER_RIGHT_6=1674,20
PRIMER_LEFT_6_TM=60.140
PRIMER_RIGHT_6_TM=59.712
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=10.0000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=779
PRIMER_PAIR_7_PENALTY=0.527992
PRIMER_LEFT_7_PENALTY=0.139753
PRIMER_RIGHT_7_PENALTY=0.388240
PRIMER_LEFT_7_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_7_SEQUENCE=TGTCTGGTTTGGTTCGGTTT
PRIMER_LEFT_7=896,20
PRIMER_RIGHT_7=1692,20
PRIMER_LEFT_7_TM=60.140
PRIMER_RIGHT_7_TM=60.388
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=10.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=797
PRIMER_PAIR_8_PENALTY=0.558627
PRIMER_LEFT_8_PENALTY=0.270338
PRIMER_RIGHT_8_PENALTY=0.288289
PRIMER_LEFT_8_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_8_SEQUENCE=TTTGACGCATTTCGTTTCAC
PRIMER_LEFT_8=898,20
PRIMER_RIGHT_8=1674,20
PRIMER_LEFT_8_TM=60.270
PRIMER_RIGHT_8_TM=59.712
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=777
PRIMER_PAIR_9_PENALTY=0.558627
PRIMER_LEFT_9_PENALTY=0.270338
PRIMER_RIGHT_9_PENALTY=0.288289
PRIMER_LEFT_9_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_9_SEQUENCE=TTTGACGCATTTCGTTTCAC
PRIMER_LEFT_9=897,20
PRIMER_RIGHT_9=1674,20
PRIMER_LEFT_9_TM=60.270
PRIMER_RIGHT_9_TM=59.712
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=778
PRIMER_PAIR_10_PENALTY=0.629623
PRIMER_LEFT_10_PENALTY=0.139753
PRIMER_RIGHT_10_PENALTY=0.489871
PRIMER_LEFT_10_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_10_SEQUENCE=GTTCGGTTTTGACGCATTTC
PRIMER_LEFT_10=896,20
PRIMER_RIGHT_10=1681,20
PRIMER_LEFT_10_TM=60.140
PRIMER_RIGHT_10_TM=60.490
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=10.0000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=786
PRIMER_PAIR_11_PENALTY=0.658578
PRIMER_LEFT_11_PENALTY=0.270338
PRIMER_RIGHT_11_PENALTY=0.388240
PRIMER_LEFT_11_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_11_SEQUENCE=TGTCTGGTTTGGTTCGGTTT
PRIMER_LEFT_11=898,20
PRIMER_RIGHT_11=1692,20
PRIMER_LEFT_11_TM=60.270
PRIMER_RIGHT_11_TM=60.388
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=795
PRIMER_PAIR_12_PENALTY=0.658578
PRIMER_LEFT_12_PENALTY=0.270338
PRIMER_RIGHT_12_PENALTY=0.388240
PRIMER_LEFT_12_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_12_SEQUENCE=TGTCTGGTTTGGTTCGGTTT
PRIMER_LEFT_12=897,20
PRIMER_RIGHT_12=1692,20
PRIMER_LEFT_12_TM=60.270
PRIMER_RIGHT_12_TM=60.388
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=796
PRIMER_PAIR_13_PENALTY=0.760208
PRIMER_LEFT_13_PENALTY=0.270338
PRIMER_RIGHT_13_PENALTY=0.489871
PRIMER_LEFT_13_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_13_SEQUENCE=GTTCGGTTTTGACGCATTTC
PRIMER_LEFT_13=898,20
PRIMER_RIGHT_13=1681,20
PRIMER_LEFT_13_TM=60.270
PRIMER_RIGHT_13_TM=60.490
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=784
PRIMER_PAIR_14_PENALTY=0.760208
PRIMER_LEFT_14_PENALTY=0.270338
PRIMER_RIGHT_14_PENALTY=0.489871
PRIMER_LEFT_14_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_14_SEQUENCE=GTTCGGTTTTGACGCATTTC
PRIMER_LEFT_14=897,20
PRIMER_RIGHT_14=1681,20
PRIMER_LEFT_14_TM=60.270
PRIMER_RIGHT_14_TM=60.490
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=785
PRIMER_PAIR_15_PENALTY=0.817945
PRIMER_LEFT_15_PENALTY=0.139753
PRIMER_RIGHT_15_PENALTY=0.678193
PRIMER_LEFT_15_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_15_SEQUENCE=TGACGCATTTCGTTTCACTT
PRIMER_LEFT_15=896,20
PRIMER_RIGHT_15=1672,20
PRIMER_LEFT_15_TM=60.140
PRIMER_RIGHT_15_TM=59.322
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.0000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=777
PRIMER_PAIR_16_PENALTY=0.817945
PRIMER_LEFT_16_PENALTY=0.139753
PRIMER_RIGHT_16_PENALTY=0.678193
PRIMER_LEFT_16_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_16_SEQUENCE=TTGACGCATTTCGTTTCACT
PRIMER_LEFT_16=896,20
PRIMER_RIGHT_16=1673,20
PRIMER_LEFT_16_TM=60.140
PRIMER_RIGHT_16_TM=59.322
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=10.0000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=778
PRIMER_PAIR_17_PENALTY=0.905346
PRIMER_LEFT_17_PENALTY=0.139753
PRIMER_RIGHT_17_PENALTY=0.765593
PRIMER_LEFT_17_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_17_SEQUENCE=TGGTTTGGTTCGGTTTTGAC
PRIMER_LEFT_17=896,20
PRIMER_RIGHT_17=1688,20
PRIMER_LEFT_17_TM=60.140
PRIMER_RIGHT_17_TM=60.766
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=10.0000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=793
PRIMER_PAIR_18_PENALTY=0.948530
PRIMER_LEFT_18_PENALTY=0.270338
PRIMER_RIGHT_18_PENALTY=0.678193
PRIMER_LEFT_18_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_18_SEQUENCE=TGACGCATTTCGTTTCACTT
PRIMER_LEFT_18=898,20
PRIMER_RIGHT_18=1672,20
PRIMER_LEFT_18_TM=60.270
PRIMER_RIGHT_18_TM=59.322
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=775
PRIMER_PAIR_19_PENALTY=0.948530
PRIMER_LEFT_19_PENALTY=0.270338
PRIMER_RIGHT_19_PENALTY=0.678193
PRIMER_LEFT_19_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_19_SEQUENCE=TTGACGCATTTCGTTTCACT
PRIMER_LEFT_19=898,20
PRIMER_RIGHT_19=1673,20
PRIMER_LEFT_19_TM=60.270
PRIMER_RIGHT_19_TM=59.322
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=776
PRIMER_PAIR_20_PENALTY=0.948530
PRIMER_LEFT_20_PENALTY=0.270338
PRIMER_RIGHT_20_PENALTY=0.678193
PRIMER_LEFT_20_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_20_SEQUENCE=TGACGCATTTCGTTTCACTT
PRIMER_LEFT_20=897,20
PRIMER_RIGHT_20=1672,20
PRIMER_LEFT_20_TM=60.270
PRIMER_RIGHT_20_TM=59.322
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=776
PRIMER_PAIR_21_PENALTY=0.948530
PRIMER_LEFT_21_PENALTY=0.270338
PRIMER_RIGHT_21_PENALTY=0.678193
PRIMER_LEFT_21_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_21_SEQUENCE=TTGACGCATTTCGTTTCACT
PRIMER_LEFT_21=897,20
PRIMER_RIGHT_21=1673,20
PRIMER_LEFT_21_TM=60.270
PRIMER_RIGHT_21_TM=59.322
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=777
PRIMER_PAIR_22_PENALTY=1.001144
PRIMER_LEFT_22_PENALTY=0.139753
PRIMER_RIGHT_22_PENALTY=0.861391
PRIMER_LEFT_22_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_22_SEQUENCE=ACGAGTCTCCAAGTCGTCCA
PRIMER_LEFT_22=896,20
PRIMER_RIGHT_22=1581,20
PRIMER_LEFT_22_TM=60.140
PRIMER_RIGHT_22_TM=60.861
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=10.0000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=686
PRIMER_PAIR_23_PENALTY=1.001144
PRIMER_LEFT_23_PENALTY=0.139753
PRIMER_RIGHT_23_PENALTY=0.861391
PRIMER_LEFT_23_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_23_SEQUENCE=CACGAGTCTCCAAGTCGTCC
PRIMER_LEFT_23=896,20
PRIMER_RIGHT_23=1582,20
PRIMER_LEFT_23_TM=60.140
PRIMER_RIGHT_23_TM=60.861
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=60.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.0000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=687
PRIMER_PAIR_24_PENALTY=1.032830
PRIMER_LEFT_24_PENALTY=0.139753
PRIMER_RIGHT_24_PENALTY=0.893078
PRIMER_LEFT_24_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_24_SEQUENCE=CTGGTTTGGTTCGGTTTTGA
PRIMER_LEFT_24=896,20
PRIMER_RIGHT_24=1689,20
PRIMER_LEFT_24_TM=60.140
PRIMER_RIGHT_24_TM=60.893
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=10.0000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=794
PRIMER_PAIR_25_PENALTY=1.032830
PRIMER_LEFT_25_PENALTY=0.139753
PRIMER_RIGHT_25_PENALTY=0.893078
PRIMER_LEFT_25_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_25_SEQUENCE=TCTGGTTTGGTTCGGTTTTG
PRIMER_LEFT_25=896,20
PRIMER_RIGHT_25=1690,20
PRIMER_LEFT_25_TM=60.140
PRIMER_RIGHT_25_TM=60.893
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=10.0000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=795
PRIMER_PAIR_26_PENALTY=1.034745
PRIMER_LEFT_26_PENALTY=0.173354
PRIMER_RIGHT_26_PENALTY=0.861391
PRIMER_LEFT_26_SEQUENCE=ATGGCCCCTTTCACCTACTT
PRIMER_RIGHT_26_SEQUENCE=ACGAGTCTCCAAGTCGTCCA
PRIMER_LEFT_26=588,20
PRIMER_RIGHT_26=1581,20
PRIMER_LEFT_26_TM=59.827
PRIMER_RIGHT_26_TM=60.861
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=5.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=994
PRIMER_PAIR_27_PENALTY=1.034745
PRIMER_LEFT_27_PENALTY=0.173354
PRIMER_RIGHT_27_PENALTY=0.861391
PRIMER_LEFT_27_SEQUENCE=ATGGCCCCTTTCACCTACTT
PRIMER_RIGHT_27_SEQUENCE=CACGAGTCTCCAAGTCGTCC
PRIMER_LEFT_27=588,20
PRIMER_RIGHT_27=1582,20
PRIMER_LEFT_27_TM=59.827
PRIMER_RIGHT_27_TM=60.861
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=60.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.7000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=995
PRIMER_PAIR_28_PENALTY=1.035931
PRIMER_LEFT_28_PENALTY=0.270338
PRIMER_RIGHT_28_PENALTY=0.765593
PRIMER_LEFT_28_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_28_SEQUENCE=TGGTTTGGTTCGGTTTTGAC
PRIMER_LEFT_28=898,20
PRIMER_RIGHT_28=1688,20
PRIMER_LEFT_28_TM=60.270
PRIMER_RIGHT_28_TM=60.766
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=791
PRIMER_PAIR_29_PENALTY=1.035931
PRIMER_LEFT_29_PENALTY=0.270338
PRIMER_RIGHT_29_PENALTY=0.765593
PRIMER_LEFT_29_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_29_SEQUENCE=TGGTTTGGTTCGGTTTTGAC
PRIMER_LEFT_29=897,20
PRIMER_RIGHT_29=1688,20
PRIMER_LEFT_29_TM=60.270
PRIMER_RIGHT_29_TM=60.766
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=792
PRIMER_PAIR_30_PENALTY=1.039142
PRIMER_LEFT_30_PENALTY=1.020705
PRIMER_RIGHT_30_PENALTY=0.018437
PRIMER_LEFT_30_SEQUENCE=CCGTAATGAGTTTGCCTGGA
PRIMER_RIGHT_30_SEQUENCE=GGTTTTGACGCATTTCGTTT
PRIMER_LEFT_30=900,20
PRIMER_RIGHT_30=1677,20
PRIMER_LEFT_30_TM=61.021
PRIMER_RIGHT_30_TM=59.982
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.7000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=778
PRIMER_PAIR_31_PENALTY=1.131729
PRIMER_LEFT_31_PENALTY=0.270338
PRIMER_RIGHT_31_PENALTY=0.861391
PRIMER_LEFT_31_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_31_SEQUENCE=ACGAGTCTCCAAGTCGTCCA
PRIMER_LEFT_31=898,20
PRIMER_RIGHT_31=1581,20
PRIMER_LEFT_31_TM=60.270
PRIMER_RIGHT_31_TM=60.861
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.7000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=684
PRIMER_PAIR_32_PENALTY=1.131729
PRIMER_LEFT_32_PENALTY=0.270338
PRIMER_RIGHT_32_PENALTY=0.861391
PRIMER_LEFT_32_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_32_SEQUENCE=CACGAGTCTCCAAGTCGTCC
PRIMER_LEFT_32=898,20
PRIMER_RIGHT_32=1582,20
PRIMER_LEFT_32_TM=60.270
PRIMER_RIGHT_32_TM=60.861
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=60.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=685
PRIMER_PAIR_33_PENALTY=1.131729
PRIMER_LEFT_33_PENALTY=0.270338
PRIMER_RIGHT_33_PENALTY=0.861391
PRIMER_LEFT_33_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_33_SEQUENCE=ACGAGTCTCCAAGTCGTCCA
PRIMER_LEFT_33=897,20
PRIMER_RIGHT_33=1581,20
PRIMER_LEFT_33_TM=60.270
PRIMER_RIGHT_33_TM=60.861
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=685
PRIMER_PAIR_34_PENALTY=1.131729
PRIMER_LEFT_34_PENALTY=0.270338
PRIMER_RIGHT_34_PENALTY=0.861391
PRIMER_LEFT_34_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_34_SEQUENCE=CACGAGTCTCCAAGTCGTCC
PRIMER_LEFT_34=897,20
PRIMER_RIGHT_34=1582,20
PRIMER_LEFT_34_TM=60.270
PRIMER_RIGHT_34_TM=60.861
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=60.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=686
PRIMER_PAIR_35_PENALTY=1.153275
PRIMER_LEFT_35_PENALTY=1.020705
PRIMER_RIGHT_35_PENALTY=0.132570
PRIMER_LEFT_35_SEQUENCE=CCGTAATGAGTTTGCCTGGA
PRIMER_RIGHT_35_SEQUENCE=GTGTCTGGTTTGGTTCGGTT
PRIMER_LEFT_35=900,20
PRIMER_RIGHT_35=1693,20
PRIMER_LEFT_35_TM=61.021
PRIMER_RIGHT_35_TM=59.867
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=794
PRIMER_PAIR_36_PENALTY=1.163415
PRIMER_LEFT_36_PENALTY=0.270338
PRIMER_RIGHT_36_PENALTY=0.893078
PRIMER_LEFT_36_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_36_SEQUENCE=CTGGTTTGGTTCGGTTTTGA
PRIMER_LEFT_36=898,20
PRIMER_RIGHT_36=1689,20
PRIMER_LEFT_36_TM=60.270
PRIMER_RIGHT_36_TM=60.893
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=792
PRIMER_PAIR_37_PENALTY=1.163415
PRIMER_LEFT_37_PENALTY=0.270338
PRIMER_RIGHT_37_PENALTY=0.893078
PRIMER_LEFT_37_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_37_SEQUENCE=TCTGGTTTGGTTCGGTTTTG
PRIMER_LEFT_37=898,20
PRIMER_RIGHT_37=1690,20
PRIMER_LEFT_37_TM=60.270
PRIMER_RIGHT_37_TM=60.893
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=793
PRIMER_PAIR_38_PENALTY=1.163415
PRIMER_LEFT_38_PENALTY=0.270338
PRIMER_RIGHT_38_PENALTY=0.893078
PRIMER_LEFT_38_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_38_SEQUENCE=CTGGTTTGGTTCGGTTTTGA
PRIMER_LEFT_38=897,20
PRIMER_RIGHT_38=1689,20
PRIMER_LEFT_38_TM=60.270
PRIMER_RIGHT_38_TM=60.893
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=7.3000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=793
PRIMER_PAIR_39_PENALTY=1.163415
PRIMER_LEFT_39_PENALTY=0.270338
PRIMER_RIGHT_39_PENALTY=0.893078
PRIMER_LEFT_39_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_39_SEQUENCE=TCTGGTTTGGTTCGGTTTTG
PRIMER_LEFT_39=897,20
PRIMER_RIGHT_39=1690,20
PRIMER_LEFT_39_TM=60.270
PRIMER_RIGHT_39_TM=60.893
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=794
PRIMER_PAIR_40_PENALTY=1.163471
PRIMER_LEFT_40_PENALTY=1.145034
PRIMER_RIGHT_40_PENALTY=0.018437
PRIMER_LEFT_40_SEQUENCE=TACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_40_SEQUENCE=GGTTTTGACGCATTTCGTTT
PRIMER_LEFT_40=895,21
PRIMER_RIGHT_40=1677,20
PRIMER_LEFT_40_TM=60.145
PRIMER_RIGHT_40_TM=59.982
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=10.0000
PRIMER_RIGHT_40_END_STABILITY=8.7000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=783
PRIMER_PAIR_41_PENALTY=1.206118
PRIMER_LEFT_41_PENALTY=0.139753
PRIMER_RIGHT_41_PENALTY=1.066365
PRIMER_LEFT_41_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_41_SEQUENCE=GTCTGGTTTGGTTCGGTTTT
PRIMER_LEFT_41=896,20
PRIMER_RIGHT_41=1691,20
PRIMER_LEFT_41_TM=60.140
PRIMER_RIGHT_41_TM=58.934
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=10.0000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=796
PRIMER_PAIR_42_PENALTY=1.259349
PRIMER_LEFT_42_PENALTY=0.139753
PRIMER_RIGHT_42_PENALTY=1.119596
PRIMER_LEFT_42_SEQUENCE=ACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_42_SEQUENCE=TCTCCTCTCACGAGTCTCCAA
PRIMER_LEFT_42=896,20
PRIMER_RIGHT_42=1590,21
PRIMER_LEFT_42_TM=60.140
PRIMER_RIGHT_42_TM=60.120
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.0000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=695
PRIMER_PAIR_43_PENALTY=1.277603
PRIMER_LEFT_43_PENALTY=1.145034
PRIMER_RIGHT_43_PENALTY=0.132570
PRIMER_LEFT_43_SEQUENCE=TACAGCCGTAATGAGTTTGCC
PRIMER_RIGHT_43_SEQUENCE=GTGTCTGGTTTGGTTCGGTT
PRIMER_LEFT_43=895,21
PRIMER_RIGHT_43=1693,20
PRIMER_LEFT_43_TM=60.145
PRIMER_RIGHT_43_TM=59.867
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.0000
PRIMER_RIGHT_43_END_STABILITY=9.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=799
PRIMER_PAIR_44_PENALTY=1.308994
PRIMER_LEFT_44_PENALTY=1.020705
PRIMER_RIGHT_44_PENALTY=0.288289
PRIMER_LEFT_44_SEQUENCE=CCGTAATGAGTTTGCCTGGA
PRIMER_RIGHT_44_SEQUENCE=TTTGACGCATTTCGTTTCAC
PRIMER_LEFT_44=900,20
PRIMER_RIGHT_44=1674,20
PRIMER_LEFT_44_TM=61.021
PRIMER_RIGHT_44_TM=59.712
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=775
PRIMER_PAIR_45_PENALTY=1.336703
PRIMER_LEFT_45_PENALTY=0.270338
PRIMER_RIGHT_45_PENALTY=1.066365
PRIMER_LEFT_45_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_45_SEQUENCE=GTCTGGTTTGGTTCGGTTTT
PRIMER_LEFT_45=898,20
PRIMER_RIGHT_45=1691,20
PRIMER_LEFT_45_TM=60.270
PRIMER_RIGHT_45_TM=58.934
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.7000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=794
PRIMER_PAIR_46_PENALTY=1.336703
PRIMER_LEFT_46_PENALTY=0.270338
PRIMER_RIGHT_46_PENALTY=1.066365
PRIMER_LEFT_46_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_46_SEQUENCE=GTCTGGTTTGGTTCGGTTTT
PRIMER_LEFT_46=897,20
PRIMER_RIGHT_46=1691,20
PRIMER_LEFT_46_TM=60.270
PRIMER_RIGHT_46_TM=58.934
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=795
PRIMER_PAIR_47_PENALTY=1.378246
PRIMER_LEFT_47_PENALTY=1.359809
PRIMER_RIGHT_47_PENALTY=0.018437
PRIMER_LEFT_47_SEQUENCE=CAGACCAAAGGTGGATGAATG
PRIMER_RIGHT_47_SEQUENCE=GGTTTTGACGCATTTCGTTT
PRIMER_LEFT_47=721,21
PRIMER_RIGHT_47=1677,20
PRIMER_LEFT_47_TM=60.360
PRIMER_RIGHT_47_TM=59.982
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=8.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=957
PRIMER_PAIR_48_PENALTY=1.389934
PRIMER_LEFT_48_PENALTY=0.270338
PRIMER_RIGHT_48_PENALTY=1.119596
PRIMER_LEFT_48_SEQUENCE=AGCCGTAATGAGTTTGCCTG
PRIMER_RIGHT_48_SEQUENCE=TCTCCTCTCACGAGTCTCCAA
PRIMER_LEFT_48=898,20
PRIMER_RIGHT_48=1590,21
PRIMER_LEFT_48_TM=60.270
PRIMER_RIGHT_48_TM=60.120
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=693
PRIMER_PAIR_49_PENALTY=1.389934
PRIMER_LEFT_49_PENALTY=0.270338
PRIMER_RIGHT_49_PENALTY=1.119596
PRIMER_LEFT_49_SEQUENCE=CAGCCGTAATGAGTTTGCCT
PRIMER_RIGHT_49_SEQUENCE=TCTCCTCTCACGAGTCTCCAA
PRIMER_LEFT_49=897,20
PRIMER_RIGHT_49=1590,21
PRIMER_LEFT_49_TM=60.270
PRIMER_RIGHT_49_TM=60.120
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=694
=
SEQUENCE_ID=MAGIC.446_4_11148529_11148935_mt-0_ler-0_126553_453495_TRUE
SEQUENCE_TARGET=950,506
SEQUENCE_TEMPLATE=AATGGTGAGCTGAGCTTGCCTTTAATGTGTTTGTTATCTATGTCGATTTCTTCATATTATGTGTGTGACATGCGACTGCGCTGCATTAGGTGTTGTANATGGCAAAATCTATGNAATCAGAGAGTGNAATAAGACAAATATGTGTTAAATAAAAAANTTTAAATTTGAGGGAGTAAANTATAAATAAAAANNTTCNACTNTAAAATAGANNAGAAAATAAAGNTNCTATATATATANAGCAATTNTGCAGAAATTTCTATTTTAGAATCNAATATAGAATANGTTTANAGAANAATTTAGAGTTTTTACACTAAAATAGANTANNNNNNNNNNNNNNNNNNNNGCATCTCCAACCNTACTNTATGTTAGAGTAAAAATGCNAAATTCTTCTTAACCCTACTCTATAATTGATTATAAAATANAGATTTTGCAGATTNACTCTATATAGAGNAACTCTATNCTCTANTNTATNNNNNAGACGACTAAACTANTTAATNCNTGTTTGCCTAAAAATTAAAANTTTTANGGCTACATTATGGTAAATCGATGGGGACACTAGTGNAAAACTAAAAATNACGTTCACTGAAAAATTTAAAATTTATGGTGAAACAANNGCGAAACACTTTGTAAAACTGAAAATTNCAGTCATTTTCACTTGACAATTAGNATTTTGAGGGATGNNNAAAANNNNNAAAAAANNNAGAATTTTGTGGTNAAGCGAAGGTGACACTAGTGCGAANCTGAAAATCGCATTTACATTCATTTGAAACTAAAATTCGTTGGTGAAACGACGACGATACTAGTGANAAAATCACAGTCATATATTATCATCTACTCGNNAAAAAAAAATCACATAACTTTTNCAATAGATTTNAAATCAAAAGCGTTGGTGATCCCGTTGTAGTATATCTCTTCGGNTAAGATAATATATAGAGAACATAGATTCGATNATCGTTTGTTGTTCAATTTATATTCTATACATGTAATCTTATTTTNAAAANTTGTTTAAATGGTAAAATNAAACTAACTAAGAAATAGTTGACGNCATTGAAAANTTACTGTTAGGAAAAATATCTGACCAACTCGANTNTACCAAATATCNAAAAANNNTCNAGTATTAAATTGGTATTGGAAGTAATTTTCACTANAAATTAGAACGGAACACGTGGTAACAGTCCANTTGGTATAGTCACATACCAATTTAATACNGGAGATTTTTTCGATATTTGGTATACTCCAGTTGGTCAGATATTTTTNNTAACAGTAACCTTTCAATGTCGTCAAACNTTTCTTCGTTAGTTTGGTTTTACCATTTAAACAANTTTTCCAAATAANATTACATGTATAGAATATAAATTGAACAACAAANGATTATNGAATCTATGTTNTCTATATATTATCTTATCNAAAGAGATATANTACAACGGGATCACCAAAGCTTTTGATTTCATATCTATTGAAAAAGGTATGTGATTTGTTTTTTCACGANTAGATNATAATATATGACTGTGATTTTCTCNCTAGTATCNCCGACGTTTCACCAACAAATTTTAGTTTCAAANGAATGCAAATGCGATTTTNAGTTTCGCACAAGTNTCATCTTCGNTTNACCNNAAAATTCTAANNNNNNNNNNTTNTTTTTCATCCCACAAAATTNTNATTGTNAAATNAAAATNGTTGGAATTTTCAGTTTTACAAAGTGTTTCNCCNTTNTTTNACCATAAATTTTAAANTTTTCAGTAAACATGATTTTTAGTTTTACGCTANTGTCACCGTANATTTACCATAATGTAGCCGTAAAATTACGTACGNTGGAGTTATTAAACTGGANAAGAGTTTAGAGTTTTCATTTCTAATCTATCGCTATCAAGTATCAATATTCATTATTATTGAACCTACCCTCGTTTCCGTCTATATCCATGATTCCAATATCATCGTGGTAATCTTATTACGAAACAAGAAACCACAGTTTTAAAGAAAACGTCAATTTGGAAAATAAAATATCATGCATGTGGTCGTCCATCAACGTTACTCATACAACTGTTAACCAANAAATTATTAACTCCTANATATGAATCGTCCTAGCACAACTTTAATATTGAGATACAAAATGCTTTTTACGTGATTACAAATCATCAGTGAAAACTCANNCTAAAATTGTTAAAGACGAATTATTTGCAGNATTACTAATCAAACTAGCAATAATGTAAATTTAGGTTTAGATTTTTGTAACATTAAAATAAGCCCACATAACCGCCATACCTAATCAATACTATTATACTAACAACAATATATAACCCTCTACCAATTGCTTAACATCAATCCAAAATAGTNCCACTNAATGATTCAGCTCCTACGACGACTGTGTAGGACAAATGCCATTCAATTATCANATCCATCTAATAT
PRIMER_LEFT_NUM_RETURNED=44
PRIMER_RIGHT_NUM_RETURNED=44
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=44
PRIMER_PAIR_0_PENALTY=2.128952
PRIMER_LEFT_0_PENALTY=1.342795
PRIMER_RIGHT_0_PENALTY=0.786156
PRIMER_LEFT_0_SEQUENCE=CGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_0_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_0=884,20
PRIMER_RIGHT_0=1548,20
PRIMER_LEFT_0_TM=61.343
PRIMER_RIGHT_0_TM=60.786
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=665
PRIMER_PAIR_1_PENALTY=3.432846
PRIMER_LEFT_1_PENALTY=2.646690
PRIMER_RIGHT_1_PENALTY=0.786156
PRIMER_LEFT_1_SEQUENCE=GTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_1_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_1=885,20
PRIMER_RIGHT_1=1548,20
PRIMER_LEFT_1_TM=57.353
PRIMER_RIGHT_1_TM=60.786
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=664
PRIMER_PAIR_2_PENALTY=3.893645
PRIMER_LEFT_2_PENALTY=3.107488
PRIMER_RIGHT_2_PENALTY=0.786156
PRIMER_LEFT_2_SEQUENCE=CGTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_2_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_2=884,21
PRIMER_RIGHT_2=1548,20
PRIMER_LEFT_2_TM=62.107
PRIMER_RIGHT_2_TM=60.786
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=665
PRIMER_PAIR_3_PENALTY=4.365876
PRIMER_LEFT_3_PENALTY=1.342795
PRIMER_RIGHT_3_PENALTY=3.023080
PRIMER_LEFT_3_SEQUENCE=CGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_3_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_3=884,20
PRIMER_RIGHT_3=1549,21
PRIMER_LEFT_3_TM=61.343
PRIMER_RIGHT_3_TM=62.023
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=666
PRIMER_PAIR_4_PENALTY=4.707667
PRIMER_LEFT_4_PENALTY=3.921510
PRIMER_RIGHT_4_PENALTY=0.786156
PRIMER_LEFT_4_SEQUENCE=AAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_4_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_4=880,20
PRIMER_RIGHT_4=1548,20
PRIMER_LEFT_4_TM=63.922
PRIMER_RIGHT_4_TM=60.786
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=669
PRIMER_PAIR_5_PENALTY=4.707667
PRIMER_LEFT_5_PENALTY=3.921510
PRIMER_RIGHT_5_PENALTY=0.786156
PRIMER_LEFT_5_SEQUENCE=AAAAGCGTTGGTGATCCCGT
PRIMER_RIGHT_5_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_5=879,20
PRIMER_RIGHT_5=1548,20
PRIMER_LEFT_5_TM=63.922
PRIMER_RIGHT_5_TM=60.786
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=11.1000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=670
PRIMER_PAIR_6_PENALTY=5.669770
PRIMER_LEFT_6_PENALTY=2.646690
PRIMER_RIGHT_6_PENALTY=3.023080
PRIMER_LEFT_6_SEQUENCE=GTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_6_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_6=885,20
PRIMER_RIGHT_6=1549,21
PRIMER_LEFT_6_TM=57.353
PRIMER_RIGHT_6_TM=62.023
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.1000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=665
PRIMER_PAIR_7_PENALTY=6.130568
PRIMER_LEFT_7_PENALTY=3.107488
PRIMER_RIGHT_7_PENALTY=3.023080
PRIMER_LEFT_7_SEQUENCE=CGTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_7_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_7=884,21
PRIMER_RIGHT_7=1549,21
PRIMER_LEFT_7_TM=62.107
PRIMER_RIGHT_7_TM=62.023
PRIMER_LEFT_7_GC_PERCENT=52.381
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=5.1000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=666
PRIMER_PAIR_8_PENALTY=6.479763
PRIMER_LEFT_8_PENALTY=1.342795
PRIMER_RIGHT_8_PENALTY=5.136967
PRIMER_LEFT_8_SEQUENCE=CGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_8_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_8=884,20
PRIMER_RIGHT_8=1550,22
PRIMER_LEFT_8_TM=61.343
PRIMER_RIGHT_8_TM=63.137
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=5.7000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=667
PRIMER_PAIR_9_PENALTY=6.639855
PRIMER_LEFT_9_PENALTY=5.853698
PRIMER_RIGHT_9_PENALTY=0.786156
PRIMER_LEFT_9_SEQUENCE=GCGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_9_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_9=883,21
PRIMER_RIGHT_9=1548,20
PRIMER_LEFT_9_TM=64.854
PRIMER_RIGHT_9_TM=60.786
PRIMER_LEFT_9_GC_PERCENT=57.143
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=666
PRIMER_PAIR_10_PENALTY=6.735872
PRIMER_LEFT_10_PENALTY=5.949715
PRIMER_RIGHT_10_PENALTY=0.786156
PRIMER_LEFT_10_SEQUENCE=AAAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_10_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_10=879,21
PRIMER_RIGHT_10=1548,20
PRIMER_LEFT_10_TM=64.950
PRIMER_RIGHT_10_TM=60.786
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.9000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=670
PRIMER_PAIR_11_PENALTY=6.839062
PRIMER_LEFT_11_PENALTY=6.052906
PRIMER_RIGHT_11_PENALTY=0.786156
PRIMER_LEFT_11_SEQUENCE=GGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_11_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_11=888,26
PRIMER_RIGHT_11=1548,20
PRIMER_LEFT_11_TM=60.053
PRIMER_RIGHT_11_TM=60.786
PRIMER_LEFT_11_GC_PERCENT=42.308
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=661
PRIMER_PAIR_12_PENALTY=6.944591
PRIMER_LEFT_12_PENALTY=3.921510
PRIMER_RIGHT_12_PENALTY=3.023080
PRIMER_LEFT_12_SEQUENCE=AAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_12_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_12=880,20
PRIMER_RIGHT_12=1549,21
PRIMER_LEFT_12_TM=63.922
PRIMER_RIGHT_12_TM=62.023
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=670
PRIMER_PAIR_13_PENALTY=6.944591
PRIMER_LEFT_13_PENALTY=3.921510
PRIMER_RIGHT_13_PENALTY=3.023080
PRIMER_LEFT_13_SEQUENCE=AAAAGCGTTGGTGATCCCGT
PRIMER_RIGHT_13_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_13=879,20
PRIMER_RIGHT_13=1549,21
PRIMER_LEFT_13_TM=63.922
PRIMER_RIGHT_13_TM=62.023
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=11.1000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=671
PRIMER_PAIR_14_PENALTY=7.783657
PRIMER_LEFT_14_PENALTY=2.646690
PRIMER_RIGHT_14_PENALTY=5.136967
PRIMER_LEFT_14_SEQUENCE=GTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_14_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_14=885,20
PRIMER_RIGHT_14=1550,22
PRIMER_LEFT_14_TM=57.353
PRIMER_RIGHT_14_TM=63.137
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=5.1000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=666
PRIMER_PAIR_15_PENALTY=8.244456
PRIMER_LEFT_15_PENALTY=3.107488
PRIMER_RIGHT_15_PENALTY=5.136967
PRIMER_LEFT_15_SEQUENCE=CGTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_15_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_15=884,21
PRIMER_RIGHT_15=1550,22
PRIMER_LEFT_15_TM=62.107
PRIMER_RIGHT_15_TM=63.137
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=5.1000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=667
PRIMER_PAIR_16_PENALTY=8.798074
PRIMER_LEFT_16_PENALTY=8.011918
PRIMER_RIGHT_16_PENALTY=0.786156
PRIMER_LEFT_16_SEQUENCE=GTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_16_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_16=889,25
PRIMER_RIGHT_16=1548,20
PRIMER_LEFT_16_TM=56.988
PRIMER_RIGHT_16_TM=60.786
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=660
PRIMER_PAIR_17_PENALTY=8.876778
PRIMER_LEFT_17_PENALTY=5.853698
PRIMER_RIGHT_17_PENALTY=3.023080
PRIMER_LEFT_17_SEQUENCE=GCGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_17_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_17=883,21
PRIMER_RIGHT_17=1549,21
PRIMER_LEFT_17_TM=64.854
PRIMER_RIGHT_17_TM=62.023
PRIMER_LEFT_17_GC_PERCENT=57.143
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=667
PRIMER_PAIR_18_PENALTY=8.972795
PRIMER_LEFT_18_PENALTY=5.949715
PRIMER_RIGHT_18_PENALTY=3.023080
PRIMER_LEFT_18_SEQUENCE=AAAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_18_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_18=879,21
PRIMER_RIGHT_18=1549,21
PRIMER_LEFT_18_TM=64.950
PRIMER_RIGHT_18_TM=62.023
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=9.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=671
PRIMER_PAIR_19_PENALTY=9.058478
PRIMER_LEFT_19_PENALTY=3.921510
PRIMER_RIGHT_19_PENALTY=5.136967
PRIMER_LEFT_19_SEQUENCE=AAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_19_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_19=880,20
PRIMER_RIGHT_19=1550,22
PRIMER_LEFT_19_TM=63.922
PRIMER_RIGHT_19_TM=63.137
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=671
PRIMER_PAIR_20_PENALTY=9.058478
PRIMER_LEFT_20_PENALTY=3.921510
PRIMER_RIGHT_20_PENALTY=5.136967
PRIMER_LEFT_20_SEQUENCE=AAAAGCGTTGGTGATCCCGT
PRIMER_RIGHT_20_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_20=879,20
PRIMER_RIGHT_20=1550,22
PRIMER_LEFT_20_TM=63.922
PRIMER_RIGHT_20_TM=63.137
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=11.1000
PRIMER_RIGHT_20_END_STABILITY=9.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=672
PRIMER_PAIR_21_PENALTY=9.075986
PRIMER_LEFT_21_PENALTY=6.052906
PRIMER_RIGHT_21_PENALTY=3.023080
PRIMER_LEFT_21_SEQUENCE=GGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_21_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_21=888,26
PRIMER_RIGHT_21=1549,21
PRIMER_LEFT_21_TM=60.053
PRIMER_RIGHT_21_TM=62.023
PRIMER_LEFT_21_GC_PERCENT=42.308
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=662
PRIMER_PAIR_22_PENALTY=10.065846
PRIMER_LEFT_22_PENALTY=9.279689
PRIMER_RIGHT_22_PENALTY=0.786156
PRIMER_LEFT_22_SEQUENCE=TGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_22_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_22=887,27
PRIMER_RIGHT_22=1548,20
PRIMER_LEFT_22_TM=62.280
PRIMER_RIGHT_22_TM=60.786
PRIMER_LEFT_22_GC_PERCENT=40.741
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=9.6000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=662
PRIMER_PAIR_23_PENALTY=10.983741
PRIMER_LEFT_23_PENALTY=1.342795
PRIMER_RIGHT_23_PENALTY=9.640946
PRIMER_LEFT_23_SEQUENCE=CGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_23_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_23=884,20
PRIMER_RIGHT_23=1553,25
PRIMER_LEFT_23_TM=61.343
PRIMER_RIGHT_23_TM=64.641
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=5.7000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=670
PRIMER_PAIR_24_PENALTY=10.990666
PRIMER_LEFT_24_PENALTY=5.853698
PRIMER_RIGHT_24_PENALTY=5.136967
PRIMER_LEFT_24_SEQUENCE=GCGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_24_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_24=883,21
PRIMER_RIGHT_24=1550,22
PRIMER_LEFT_24_TM=64.854
PRIMER_RIGHT_24_TM=63.137
PRIMER_LEFT_24_GC_PERCENT=57.143
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=5.7000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=668
PRIMER_PAIR_25_PENALTY=11.034998
PRIMER_LEFT_25_PENALTY=8.011918
PRIMER_RIGHT_25_PENALTY=3.023080
PRIMER_LEFT_25_SEQUENCE=GTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_25_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_25=889,25
PRIMER_RIGHT_25=1549,21
PRIMER_LEFT_25_TM=56.988
PRIMER_RIGHT_25_TM=62.023
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=661
PRIMER_PAIR_26_PENALTY=11.086683
PRIMER_LEFT_26_PENALTY=5.949715
PRIMER_RIGHT_26_PENALTY=5.136967
PRIMER_LEFT_26_SEQUENCE=AAAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_26_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_26=879,21
PRIMER_RIGHT_26=1550,22
PRIMER_LEFT_26_TM=64.950
PRIMER_RIGHT_26_TM=63.137
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=672
PRIMER_PAIR_27_PENALTY=11.189873
PRIMER_LEFT_27_PENALTY=6.052906
PRIMER_RIGHT_27_PENALTY=5.136967
PRIMER_LEFT_27_SEQUENCE=GGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_27_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_27=888,26
PRIMER_RIGHT_27=1550,22
PRIMER_LEFT_27_TM=60.053
PRIMER_RIGHT_27_TM=63.137
PRIMER_LEFT_27_GC_PERCENT=42.308
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=663
PRIMER_PAIR_28_PENALTY=12.287636
PRIMER_LEFT_28_PENALTY=2.646690
PRIMER_RIGHT_28_PENALTY=9.640946
PRIMER_LEFT_28_SEQUENCE=GTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_28_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_28=885,20
PRIMER_RIGHT_28=1553,25
PRIMER_LEFT_28_TM=57.353
PRIMER_RIGHT_28_TM=64.641
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=5.1000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=669
PRIMER_PAIR_29_PENALTY=12.302770
PRIMER_LEFT_29_PENALTY=9.279689
PRIMER_RIGHT_29_PENALTY=3.023080
PRIMER_LEFT_29_SEQUENCE=TGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_29_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_29=887,27
PRIMER_RIGHT_29=1549,21
PRIMER_LEFT_29_TM=62.280
PRIMER_RIGHT_29_TM=62.023
PRIMER_LEFT_29_GC_PERCENT=40.741
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=663
PRIMER_PAIR_30_PENALTY=12.748434
PRIMER_LEFT_30_PENALTY=3.107488
PRIMER_RIGHT_30_PENALTY=9.640946
PRIMER_LEFT_30_SEQUENCE=CGTTGGTGATCCCGTTGTAGT
PRIMER_RIGHT_30_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_30=884,21
PRIMER_RIGHT_30=1553,25
PRIMER_LEFT_30_TM=62.107
PRIMER_RIGHT_30_TM=64.641
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=5.1000
PRIMER_RIGHT_30_END_STABILITY=9.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=670
PRIMER_PAIR_31_PENALTY=13.148885
PRIMER_LEFT_31_PENALTY=8.011918
PRIMER_RIGHT_31_PENALTY=5.136967
PRIMER_LEFT_31_SEQUENCE=GTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_31_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_31=889,25
PRIMER_RIGHT_31=1550,22
PRIMER_LEFT_31_TM=56.988
PRIMER_RIGHT_31_TM=63.137
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=662
PRIMER_PAIR_32_PENALTY=13.513279
PRIMER_LEFT_32_PENALTY=12.727122
PRIMER_RIGHT_32_PENALTY=0.786156
PRIMER_LEFT_32_SEQUENCE=GTTGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_32_SEQUENCE=ATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_32=885,29
PRIMER_RIGHT_32=1548,20
PRIMER_LEFT_32_TM=63.727
PRIMER_RIGHT_32_TM=60.786
PRIMER_LEFT_32_GC_PERCENT=41.379
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=664
PRIMER_PAIR_33_PENALTY=13.562456
PRIMER_LEFT_33_PENALTY=3.921510
PRIMER_RIGHT_33_PENALTY=9.640946
PRIMER_LEFT_33_SEQUENCE=AAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_33_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_33=880,20
PRIMER_RIGHT_33=1553,25
PRIMER_LEFT_33_TM=63.922
PRIMER_RIGHT_33_TM=64.641
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.9000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=674
PRIMER_PAIR_34_PENALTY=13.562456
PRIMER_LEFT_34_PENALTY=3.921510
PRIMER_RIGHT_34_PENALTY=9.640946
PRIMER_LEFT_34_SEQUENCE=AAAAGCGTTGGTGATCCCGT
PRIMER_RIGHT_34_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_34=879,20
PRIMER_RIGHT_34=1553,25
PRIMER_LEFT_34_TM=63.922
PRIMER_RIGHT_34_TM=64.641
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=11.1000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=675
PRIMER_PAIR_35_PENALTY=14.416657
PRIMER_LEFT_35_PENALTY=9.279689
PRIMER_RIGHT_35_PENALTY=5.136967
PRIMER_LEFT_35_SEQUENCE=TGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_35_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_35=887,27
PRIMER_RIGHT_35=1550,22
PRIMER_LEFT_35_TM=62.280
PRIMER_RIGHT_35_TM=63.137
PRIMER_LEFT_35_GC_PERCENT=40.741
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=9.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=664
PRIMER_PAIR_36_PENALTY=15.494644
PRIMER_LEFT_36_PENALTY=5.853698
PRIMER_RIGHT_36_PENALTY=9.640946
PRIMER_LEFT_36_SEQUENCE=GCGTTGGTGATCCCGTTGTAG
PRIMER_RIGHT_36_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_36=883,21
PRIMER_RIGHT_36=1553,25
PRIMER_LEFT_36_TM=64.854
PRIMER_RIGHT_36_TM=64.641
PRIMER_LEFT_36_GC_PERCENT=57.143
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=5.7000
PRIMER_RIGHT_36_END_STABILITY=9.6000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=671
PRIMER_PAIR_37_PENALTY=15.590661
PRIMER_LEFT_37_PENALTY=5.949715
PRIMER_RIGHT_37_PENALTY=9.640946
PRIMER_LEFT_37_SEQUENCE=AAAAGCGTTGGTGATCCCGTT
PRIMER_RIGHT_37_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_37=879,21
PRIMER_RIGHT_37=1553,25
PRIMER_LEFT_37_TM=64.950
PRIMER_RIGHT_37_TM=64.641
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.9000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=675
PRIMER_PAIR_38_PENALTY=15.693852
PRIMER_LEFT_38_PENALTY=6.052906
PRIMER_RIGHT_38_PENALTY=9.640946
PRIMER_LEFT_38_SEQUENCE=GGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_38_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_38=888,26
PRIMER_RIGHT_38=1553,25
PRIMER_LEFT_38_TM=60.053
PRIMER_RIGHT_38_TM=64.641
PRIMER_LEFT_38_GC_PERCENT=42.308
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=666
PRIMER_PAIR_39_PENALTY=15.750202
PRIMER_LEFT_39_PENALTY=12.727122
PRIMER_RIGHT_39_PENALTY=3.023080
PRIMER_LEFT_39_SEQUENCE=GTTGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_39_SEQUENCE=AATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_39=885,29
PRIMER_RIGHT_39=1549,21
PRIMER_LEFT_39_TM=63.727
PRIMER_RIGHT_39_TM=62.023
PRIMER_LEFT_39_GC_PERCENT=41.379
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=665
PRIMER_PAIR_40_PENALTY=17.652864
PRIMER_LEFT_40_PENALTY=8.011918
PRIMER_RIGHT_40_PENALTY=9.640946
PRIMER_LEFT_40_SEQUENCE=GTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_40_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_40=889,25
PRIMER_RIGHT_40=1553,25
PRIMER_LEFT_40_TM=56.988
PRIMER_RIGHT_40_TM=64.641
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=9.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=665
PRIMER_PAIR_41_PENALTY=17.864090
PRIMER_LEFT_41_PENALTY=12.727122
PRIMER_RIGHT_41_PENALTY=5.136967
PRIMER_LEFT_41_SEQUENCE=GTTGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_41_SEQUENCE=AAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_41=885,29
PRIMER_RIGHT_41=1550,22
PRIMER_LEFT_41_TM=63.727
PRIMER_RIGHT_41_TM=63.137
PRIMER_LEFT_41_GC_PERCENT=41.379
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=666
PRIMER_PAIR_42_PENALTY=18.920635
PRIMER_LEFT_42_PENALTY=9.279689
PRIMER_RIGHT_42_PENALTY=9.640946
PRIMER_LEFT_42_SEQUENCE=TGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_42_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_42=887,27
PRIMER_RIGHT_42=1553,25
PRIMER_LEFT_42_TM=62.280
PRIMER_RIGHT_42_TM=64.641
PRIMER_LEFT_42_GC_PERCENT=40.741
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=667
PRIMER_PAIR_43_PENALTY=22.368068
PRIMER_LEFT_43_PENALTY=12.727122
PRIMER_RIGHT_43_PENALTY=9.640946
PRIMER_LEFT_43_SEQUENCE=GTTGGTGATCCCGTTGTAGTATATCTCTT
PRIMER_RIGHT_43_SEQUENCE=CTAAAATTTGTTGGTGAAACGTCGG
PRIMER_LEFT_43=885,29
PRIMER_RIGHT_43=1553,25
PRIMER_LEFT_43_TM=63.727
PRIMER_RIGHT_43_TM=64.641
PRIMER_LEFT_43_GC_PERCENT=41.379
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=669
=
SEQUENCE_ID=MAGIC.175_2_626348_626402_ct-1_edi-0_7783_397135_TRUE
SEQUENCE_TARGET=950,154
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCANTAANGGNTTNATTTTTAACAATTCAAAATTNTTGAATNCGTTNAACACCGTTGAATCNACATAANATTCATTTNTTTATTNAATNTTNNACTNTAAGCATTCAANTATTNAANTANTTTTTTNAATANNCNATNGTANATNGTNTTAAAAAGGGNNNNNNNNNNGTGAATGAATNGATTGTATTTTCTTNAAATAATGTGTAAGTCATACGTGACAATTTCACAACAAAAATATTTNTATGGACAAAAGAAATTTGGTAGACATATTTGATATTCCCATTAAAAAATTGCATGAGAAAAAGANNNNATATGAAATGGTTTGGTTTCCTTTCTAAACAANAAAAAAAAAAGTAATCTCCGAAGAAGAACAAATTCCTTGCTGAATNATGGTTAATTTCGAATCTCTTTCTATCTAATTTTATAAATGTGATGATAACAGATCATCGTCTCCACTCAGTTTCCGTTTGATANNCTTATGTTACCGATTTTTNTCAGGCGAAGTTGAAGCTCTACTCTTACTGGAGAAGCTCATGTGCTCATCGCGTCCGTNTCGCCCTCACTTTANAAGGTNCCAGCCAATGATTTTATTCTTTTCTTGTGAGCAATTCTTTGATCTGAATTTGGTTCTTGTTCGATTTTCATTAGGGCTTGATTATGAATATATACCGGTTAATTTGCTCAAAGGGGATCAATCCGATTCAGGTGCGTANTTTCTAGGTTNTATTGAACTTTATTTGAAGTAACATTGTAAAGATAAGAATGGTAAGTAACTGAGATTTCNTATNTTAGACTTAGAAGTTTATNCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCCAATGGGCACNGTACCAGCGCTTGTTGATGGTGATGTTGTGATNAATGACTCTTTCGCAATNATAATGGTCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14328857_14328998_po-0_no-0_260864_16649_TRUE
SEQUENCE_TARGET=950,241
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGGCCTGTGGGGATATAAGNNNTAACTTCTTCGTTGGACGTGCAATTCTTAAACCTGACGGCTCTTCTAATTGCAGCCCTTGCTTTCCTCAACTCTTCTTCTCTTTTCTCAAGATTGCTCCTTCTCTGNCGACATTTCATTTAATTTTGTTATTACCTTNACTTCATTTTNACTACTACAAACTANGACNNTTTTCTATGNNTNTATGCATACTATATATAATATNNACATGTACATGTCAAGTTGACCAGAATAAAAGATTAGTTATGTACGTTCCNAAAGTCGTANGAATCATTACAATATACATGNACATCGACATCGAGTACTAATATAGCAAATGACNAATTCAAATAATATATAACACATGCATGAATCATCGGAAANTCATGTATTGANTTNGAGANTTTCACCATATAGCATGTAGATTTGCAAACGTTAATATATGAAAACATCAGTATAGTAATNAAAGAGGAAANCTNTACATATGAATCNNAGAGATTCTTACCTTNACGGTAATCGGAGGAGAGTATAANGAAGAAGAAGAAGATGAGGATTGTAATGCATTTGTGTGANCAAGAAGAGAGGACATAGTAAGTGAAGAGAAGAACTGTTGAGNTNGAGATTCATTATTAGGGAACGAAGAAAAAANTANGAAGNNNAGAAGAAGAATAAGAANCAATGGAAACCCTAACAAGCAAAAANTCTTGNAAGAATNGTTTGACATGTTTTTNCTTTTGCTAAATGCTTTCTCTCGTTGTTCACTTTCATTTGGCCTCTTGAACCATTTATATGCGACNTCGAGCAGTTCATGTTTGACTTTGNGAGTTTTTTATACCAAAATAATATTTTTANACTAACAAAGCCNTTTTAGTCGACGTCATAGCTTCTTCCTAGACGCAAAATCTTTTNNAGGTGATTACTTTAGAATGGTNTAGTNTAATCCGNTGGTTTTNTCGGNNAATTAGANATTTAATTGNTATGGAAANNNNTAATGGCANATATANTCATGTATTTATGTGATTGNGAAGACAATAAAATAANTTTCANTANATATATATNCTANAATCAATTACTAATNNCAACAAATTTGNGAGGAAATTNGATTNAGTANGAAAANTTTGANAGATATATAACTTTNCATTTNNANNNANNNTNANNTGTTGGATCAAATTTTNTTAAGTCAANTGGGNTTNATAAAATTNAATTGTGTGAATGAGAANTGGNNNNNNNNNNNNNNGTTTGTTCTCACNGNAGCTCCANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANAANNNNNNTCNTTNGTTTCACGTNTCCTTGAGACTCGGNTTCNTTGTGAGATCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTTGTGGTTGATNGATCTTNAAAATNAAACCAATCTCAAACCNCNNTATATGGACGTTTGAGGTCTTTTTCTTAAGGGACTGAGAATTTCATATCGCTCATATAGAAACACATATNTCCAGCAATTTCTCTTTGNTAGAAAGTNNTNAANAGTNTCGTNNNNNNANTTGGTTCGTTAGAGCTGNTNNCCGNNNNNAGTTTATANAGGTTGTATCNTGNNATCCTTGAATCACACANAAACCGTCANACNANGGNTGATTCAAAGGGTTAAGGANAGAGTTCATNAACTCGACTNCTNTCTTNTCTTTNNGTANCATTTNTGNTTTNNATTNNAANNNNTTTACGTAGTGNTTNNTAATTTTAGCTCCTACNGNNTNAANCCTNTNNNNNNGNTTTGTTCTANAAACATNAACNGATTGNAATTNNANNTTNATAAACGTGGGNAGNNNGTGAGTGTGTACATTGAGTTTANTGGTTGCGANTGGTAAACGTCNAANNNNTTNTGNCNANTNGCGAGNNTNGACTTNGTTNTTCTTTNGGAAACAGAGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.846755
PRIMER_LEFT_0_PENALTY=0.051345
PRIMER_RIGHT_0_PENALTY=1.795410
PRIMER_LEFT_0_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_0_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_0=827,20
PRIMER_RIGHT_0=1522,21
PRIMER_LEFT_0_TM=60.051
PRIMER_RIGHT_0_TM=59.205
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=696
PRIMER_PAIR_1_PENALTY=2.195313
PRIMER_LEFT_1_PENALTY=0.051345
PRIMER_RIGHT_1_PENALTY=2.143968
PRIMER_LEFT_1_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_1_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_1=827,20
PRIMER_RIGHT_1=1523,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=697
PRIMER_PAIR_2_PENALTY=2.749791
PRIMER_LEFT_2_PENALTY=0.051345
PRIMER_RIGHT_2_PENALTY=2.698446
PRIMER_LEFT_2_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_2_SEQUENCE=AAAAAGACCTCAAACGTCCA
PRIMER_LEFT_2=827,20
PRIMER_RIGHT_2=1521,20
PRIMER_LEFT_2_TM=60.051
PRIMER_RIGHT_2_TM=57.302
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=695
PRIMER_PAIR_3_PENALTY=2.813098
PRIMER_LEFT_3_PENALTY=1.017688
PRIMER_RIGHT_3_PENALTY=1.795410
PRIMER_LEFT_3_SEQUENCE=CAATGGAAACCCTAACAAGCA
PRIMER_RIGHT_3_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_3=740,21
PRIMER_RIGHT_3=1522,21
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=59.205
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=783
PRIMER_PAIR_4_PENALTY=2.898515
PRIMER_LEFT_4_PENALTY=1.103105
PRIMER_RIGHT_4_PENALTY=1.795410
PRIMER_LEFT_4_SEQUENCE=TGTTCACTTTCATTTGGCCTC
PRIMER_RIGHT_4_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_4=819,21
PRIMER_RIGHT_4=1522,21
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=59.205
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=704
PRIMER_PAIR_5_PENALTY=3.161656
PRIMER_LEFT_5_PENALTY=1.017688
PRIMER_RIGHT_5_PENALTY=2.143968
PRIMER_LEFT_5_SEQUENCE=CAATGGAAACCCTAACAAGCA
PRIMER_RIGHT_5_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_5=740,21
PRIMER_RIGHT_5=1523,22
PRIMER_LEFT_5_TM=59.982
PRIMER_RIGHT_5_TM=60.144
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=784
PRIMER_PAIR_6_PENALTY=3.247073
PRIMER_LEFT_6_PENALTY=1.103105
PRIMER_RIGHT_6_PENALTY=2.143968
PRIMER_LEFT_6_SEQUENCE=TGTTCACTTTCATTTGGCCTC
PRIMER_RIGHT_6_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_6=819,21
PRIMER_RIGHT_6=1523,22
PRIMER_LEFT_6_TM=60.103
PRIMER_RIGHT_6_TM=60.144
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=705
PRIMER_PAIR_7_PENALTY=3.247164
PRIMER_LEFT_7_PENALTY=0.051345
PRIMER_RIGHT_7_PENALTY=3.195819
PRIMER_LEFT_7_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_7_SEQUENCE=AAGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_7=827,20
PRIMER_RIGHT_7=1524,22
PRIMER_LEFT_7_TM=60.051
PRIMER_RIGHT_7_TM=58.804
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=698
PRIMER_PAIR_8_PENALTY=3.291425
PRIMER_LEFT_8_PENALTY=1.496015
PRIMER_RIGHT_8_PENALTY=1.795410
PRIMER_LEFT_8_SEQUENCE=ATTTGGCCTCTTGAACCATT
PRIMER_RIGHT_8_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_8=830,20
PRIMER_RIGHT_8=1522,21
PRIMER_LEFT_8_TM=58.504
PRIMER_RIGHT_8_TM=59.205
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=693
PRIMER_PAIR_9_PENALTY=3.528398
PRIMER_LEFT_9_PENALTY=1.732988
PRIMER_RIGHT_9_PENALTY=1.795410
PRIMER_LEFT_9_SEQUENCE=TGGAAACCCTAACAAGCAAA
PRIMER_RIGHT_9_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_9=743,20
PRIMER_RIGHT_9=1522,21
PRIMER_LEFT_9_TM=58.267
PRIMER_RIGHT_9_TM=59.205
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.8000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=780
PRIMER_PAIR_10_PENALTY=3.603796
PRIMER_LEFT_10_PENALTY=0.051345
PRIMER_RIGHT_10_PENALTY=3.552451
PRIMER_LEFT_10_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_10_SEQUENCE=AGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_10=827,20
PRIMER_RIGHT_10=1523,21
PRIMER_LEFT_10_TM=60.051
PRIMER_RIGHT_10_TM=57.448
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=697
PRIMER_PAIR_11_PENALTY=3.639983
PRIMER_LEFT_11_PENALTY=1.496015
PRIMER_RIGHT_11_PENALTY=2.143968
PRIMER_LEFT_11_SEQUENCE=ATTTGGCCTCTTGAACCATT
PRIMER_RIGHT_11_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_11=830,20
PRIMER_RIGHT_11=1523,22
PRIMER_LEFT_11_TM=58.504
PRIMER_RIGHT_11_TM=60.144
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=694
PRIMER_PAIR_12_PENALTY=3.649866
PRIMER_LEFT_12_PENALTY=0.454047
PRIMER_RIGHT_12_PENALTY=3.195819
PRIMER_LEFT_12_SEQUENCE=TTTGGCCTCTTGAACCATTT
PRIMER_RIGHT_12_SEQUENCE=AAGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_12=831,20
PRIMER_RIGHT_12=1524,22
PRIMER_LEFT_12_TM=59.546
PRIMER_RIGHT_12_TM=58.804
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=694
PRIMER_PAIR_13_PENALTY=3.700921
PRIMER_LEFT_13_PENALTY=0.051345
PRIMER_RIGHT_13_PENALTY=3.649576
PRIMER_LEFT_13_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_13_SEQUENCE=GAAAAAGACCTCAAACGTCC
PRIMER_LEFT_13=827,20
PRIMER_RIGHT_13=1522,20
PRIMER_LEFT_13_TM=60.051
PRIMER_RIGHT_13_TM=56.350
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=696
PRIMER_PAIR_14_PENALTY=3.716135
PRIMER_LEFT_14_PENALTY=1.017688
PRIMER_RIGHT_14_PENALTY=2.698446
PRIMER_LEFT_14_SEQUENCE=CAATGGAAACCCTAACAAGCA
PRIMER_RIGHT_14_SEQUENCE=AAAAAGACCTCAAACGTCCA
PRIMER_LEFT_14=740,21
PRIMER_RIGHT_14=1521,20
PRIMER_LEFT_14_TM=59.982
PRIMER_RIGHT_14_TM=57.302
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=782
PRIMER_PAIR_15_PENALTY=3.801551
PRIMER_LEFT_15_PENALTY=1.103105
PRIMER_RIGHT_15_PENALTY=2.698446
PRIMER_LEFT_15_SEQUENCE=TGTTCACTTTCATTTGGCCTC
PRIMER_RIGHT_15_SEQUENCE=AAAAAGACCTCAAACGTCCA
PRIMER_LEFT_15=819,21
PRIMER_RIGHT_15=1521,20
PRIMER_LEFT_15_TM=60.103
PRIMER_RIGHT_15_TM=57.302
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=703
PRIMER_PAIR_16_PENALTY=3.876956
PRIMER_LEFT_16_PENALTY=1.732988
PRIMER_RIGHT_16_PENALTY=2.143968
PRIMER_LEFT_16_SEQUENCE=TGGAAACCCTAACAAGCAAA
PRIMER_RIGHT_16_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_16=743,20
PRIMER_RIGHT_16=1523,22
PRIMER_LEFT_16_TM=58.267
PRIMER_RIGHT_16_TM=60.144
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.8000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=781
PRIMER_PAIR_17_PENALTY=4.006498
PRIMER_LEFT_17_PENALTY=0.454047
PRIMER_RIGHT_17_PENALTY=3.552451
PRIMER_LEFT_17_SEQUENCE=TTTGGCCTCTTGAACCATTT
PRIMER_RIGHT_17_SEQUENCE=AGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_17=831,20
PRIMER_RIGHT_17=1523,21
PRIMER_LEFT_17_TM=59.546
PRIMER_RIGHT_17_TM=57.448
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.2000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=693
PRIMER_PAIR_18_PENALTY=4.009014
PRIMER_LEFT_18_PENALTY=2.213604
PRIMER_RIGHT_18_PENALTY=1.795410
PRIMER_LEFT_18_SEQUENCE=CATTTGGCCTCTTGAACCATT
PRIMER_RIGHT_18_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_18=829,21
PRIMER_RIGHT_18=1522,21
PRIMER_LEFT_18_TM=61.214
PRIMER_RIGHT_18_TM=59.205
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=694
PRIMER_PAIR_19_PENALTY=4.103623
PRIMER_LEFT_19_PENALTY=0.454047
PRIMER_RIGHT_19_PENALTY=3.649576
PRIMER_LEFT_19_SEQUENCE=TTTGGCCTCTTGAACCATTT
PRIMER_RIGHT_19_SEQUENCE=GAAAAAGACCTCAAACGTCC
PRIMER_LEFT_19=831,20
PRIMER_RIGHT_19=1522,20
PRIMER_LEFT_19_TM=59.546
PRIMER_RIGHT_19_TM=56.350
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=692
PRIMER_PAIR_20_PENALTY=4.126664
PRIMER_LEFT_20_PENALTY=2.331254
PRIMER_RIGHT_20_PENALTY=1.795410
PRIMER_LEFT_20_SEQUENCE=TGCTAAATGCTTTCTCTCGTTG
PRIMER_RIGHT_20_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_20=799,22
PRIMER_RIGHT_20=1522,21
PRIMER_LEFT_20_TM=59.669
PRIMER_RIGHT_20_TM=59.205
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.7000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=724
PRIMER_PAIR_21_PENALTY=4.146585
PRIMER_LEFT_21_PENALTY=2.351175
PRIMER_RIGHT_21_PENALTY=1.795410
PRIMER_LEFT_21_SEQUENCE=CTCGTTGTTCACTTTCATTTGG
PRIMER_RIGHT_21_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_21=814,22
PRIMER_RIGHT_21=1522,21
PRIMER_LEFT_21_TM=59.649
PRIMER_RIGHT_21_TM=59.205
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.8000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=709
PRIMER_PAIR_22_PENALTY=4.158846
PRIMER_LEFT_22_PENALTY=2.363436
PRIMER_RIGHT_22_PENALTY=1.795410
PRIMER_LEFT_22_SEQUENCE=GTTCACTTTCATTTGGCCTCTT
PRIMER_RIGHT_22_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_22=820,22
PRIMER_RIGHT_22=1522,21
PRIMER_LEFT_22_TM=59.637
PRIMER_RIGHT_22_TM=59.205
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=703
PRIMER_PAIR_23_PENALTY=4.194462
PRIMER_LEFT_23_PENALTY=1.496015
PRIMER_RIGHT_23_PENALTY=2.698446
PRIMER_LEFT_23_SEQUENCE=ATTTGGCCTCTTGAACCATT
PRIMER_RIGHT_23_SEQUENCE=AAAAAGACCTCAAACGTCCA
PRIMER_LEFT_23=830,20
PRIMER_RIGHT_23=1521,20
PRIMER_LEFT_23_TM=58.504
PRIMER_RIGHT_23_TM=57.302
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=692
PRIMER_PAIR_24_PENALTY=4.203923
PRIMER_LEFT_24_PENALTY=0.051345
PRIMER_RIGHT_24_PENALTY=4.152578
PRIMER_LEFT_24_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_24_SEQUENCE=CAGTCCCTTAAGAAAAAGACCTCA
PRIMER_LEFT_24=827,20
PRIMER_RIGHT_24=1533,24
PRIMER_LEFT_24_TM=60.051
PRIMER_RIGHT_24_TM=60.153
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=41.667
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=707
PRIMER_PAIR_25_PENALTY=4.213508
PRIMER_LEFT_25_PENALTY=1.017688
PRIMER_RIGHT_25_PENALTY=3.195819
PRIMER_LEFT_25_SEQUENCE=CAATGGAAACCCTAACAAGCA
PRIMER_RIGHT_25_SEQUENCE=AAGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_25=740,21
PRIMER_RIGHT_25=1524,22
PRIMER_LEFT_25_TM=59.982
PRIMER_RIGHT_25_TM=58.804
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=785
PRIMER_PAIR_26_PENALTY=4.298924
PRIMER_LEFT_26_PENALTY=1.103105
PRIMER_RIGHT_26_PENALTY=3.195819
PRIMER_LEFT_26_SEQUENCE=TGTTCACTTTCATTTGGCCTC
PRIMER_RIGHT_26_SEQUENCE=AAGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_26=819,21
PRIMER_RIGHT_26=1524,22
PRIMER_LEFT_26_TM=60.103
PRIMER_RIGHT_26_TM=58.804
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=706
PRIMER_PAIR_27_PENALTY=4.357572
PRIMER_LEFT_27_PENALTY=2.213604
PRIMER_RIGHT_27_PENALTY=2.143968
PRIMER_LEFT_27_SEQUENCE=CATTTGGCCTCTTGAACCATT
PRIMER_RIGHT_27_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_27=829,21
PRIMER_RIGHT_27=1523,22
PRIMER_LEFT_27_TM=61.214
PRIMER_RIGHT_27_TM=60.144
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=695
PRIMER_PAIR_28_PENALTY=4.402383
PRIMER_LEFT_28_PENALTY=2.606973
PRIMER_RIGHT_28_PENALTY=1.795410
PRIMER_LEFT_28_SEQUENCE=TCACTTTCATTTGGCCTCTT
PRIMER_RIGHT_28_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_28=822,20
PRIMER_RIGHT_28=1522,21
PRIMER_LEFT_28_TM=57.393
PRIMER_RIGHT_28_TM=59.205
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=701
PRIMER_PAIR_29_PENALTY=4.402383
PRIMER_LEFT_29_PENALTY=2.606973
PRIMER_RIGHT_29_PENALTY=1.795410
PRIMER_LEFT_29_SEQUENCE=TTCACTTTCATTTGGCCTCT
PRIMER_RIGHT_29_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_29=821,20
PRIMER_RIGHT_29=1522,21
PRIMER_LEFT_29_TM=57.393
PRIMER_RIGHT_29_TM=59.205
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=702
PRIMER_PAIR_30_PENALTY=4.431434
PRIMER_LEFT_30_PENALTY=1.732988
PRIMER_RIGHT_30_PENALTY=2.698446
PRIMER_LEFT_30_SEQUENCE=TGGAAACCCTAACAAGCAAA
PRIMER_RIGHT_30_SEQUENCE=AAAAAGACCTCAAACGTCCA
PRIMER_LEFT_30=743,20
PRIMER_RIGHT_30=1521,20
PRIMER_LEFT_30_TM=58.267
PRIMER_RIGHT_30_TM=57.302
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.8000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=779
PRIMER_PAIR_31_PENALTY=4.475222
PRIMER_LEFT_31_PENALTY=2.331254
PRIMER_RIGHT_31_PENALTY=2.143968
PRIMER_LEFT_31_SEQUENCE=TGCTAAATGCTTTCTCTCGTTG
PRIMER_RIGHT_31_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_31=799,22
PRIMER_RIGHT_31=1523,22
PRIMER_LEFT_31_TM=59.669
PRIMER_RIGHT_31_TM=60.144
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.7000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=725
PRIMER_PAIR_32_PENALTY=4.489601
PRIMER_LEFT_32_PENALTY=2.694191
PRIMER_RIGHT_32_PENALTY=1.795410
PRIMER_LEFT_32_SEQUENCE=GTTCACTTTCATTTGGCCTCT
PRIMER_RIGHT_32_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_32=820,21
PRIMER_RIGHT_32=1522,21
PRIMER_LEFT_32_TM=58.306
PRIMER_RIGHT_32_TM=59.205
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=703
PRIMER_PAIR_33_PENALTY=4.495143
PRIMER_LEFT_33_PENALTY=2.351175
PRIMER_RIGHT_33_PENALTY=2.143968
PRIMER_LEFT_33_SEQUENCE=CTCGTTGTTCACTTTCATTTGG
PRIMER_RIGHT_33_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_33=814,22
PRIMER_RIGHT_33=1523,22
PRIMER_LEFT_33_TM=59.649
PRIMER_RIGHT_33_TM=60.144
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=40.909
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.8000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=710
PRIMER_PAIR_34_PENALTY=4.507404
PRIMER_LEFT_34_PENALTY=2.363436
PRIMER_RIGHT_34_PENALTY=2.143968
PRIMER_LEFT_34_SEQUENCE=GTTCACTTTCATTTGGCCTCTT
PRIMER_RIGHT_34_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_34=820,22
PRIMER_RIGHT_34=1523,22
PRIMER_LEFT_34_TM=59.637
PRIMER_RIGHT_34_TM=60.144
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=704
PRIMER_PAIR_35_PENALTY=4.560797
PRIMER_LEFT_35_PENALTY=2.765387
PRIMER_RIGHT_35_PENALTY=1.795410
PRIMER_LEFT_35_SEQUENCE=GTTCACTTTCATTTGGCCTC
PRIMER_RIGHT_35_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_35=820,20
PRIMER_RIGHT_35=1522,21
PRIMER_LEFT_35_TM=57.235
PRIMER_RIGHT_35_TM=59.205
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=703
PRIMER_PAIR_36_PENALTY=4.570140
PRIMER_LEFT_36_PENALTY=1.017688
PRIMER_RIGHT_36_PENALTY=3.552451
PRIMER_LEFT_36_SEQUENCE=CAATGGAAACCCTAACAAGCA
PRIMER_RIGHT_36_SEQUENCE=AGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_36=740,21
PRIMER_RIGHT_36=1523,21
PRIMER_LEFT_36_TM=59.982
PRIMER_RIGHT_36_TM=57.448
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=9.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=784
PRIMER_PAIR_37_PENALTY=4.578188
PRIMER_LEFT_37_PENALTY=2.782778
PRIMER_RIGHT_37_PENALTY=1.795410
PRIMER_LEFT_37_SEQUENCE=ATGGAAACCCTAACAAGCAA
PRIMER_RIGHT_37_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_37=742,20
PRIMER_RIGHT_37=1522,21
PRIMER_LEFT_37_TM=57.217
PRIMER_RIGHT_37_TM=59.205
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=781
PRIMER_PAIR_38_PENALTY=4.578188
PRIMER_LEFT_38_PENALTY=2.782778
PRIMER_RIGHT_38_PENALTY=1.795410
PRIMER_LEFT_38_SEQUENCE=AATGGAAACCCTAACAAGCA
PRIMER_RIGHT_38_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_38=741,20
PRIMER_RIGHT_38=1522,21
PRIMER_LEFT_38_TM=57.217
PRIMER_RIGHT_38_TM=59.205
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=782
PRIMER_PAIR_39_PENALTY=4.606625
PRIMER_LEFT_39_PENALTY=0.454047
PRIMER_RIGHT_39_PENALTY=4.152578
PRIMER_LEFT_39_SEQUENCE=TTTGGCCTCTTGAACCATTT
PRIMER_RIGHT_39_SEQUENCE=CAGTCCCTTAAGAAAAAGACCTCA
PRIMER_LEFT_39=831,20
PRIMER_RIGHT_39=1533,24
PRIMER_LEFT_39_TM=59.546
PRIMER_RIGHT_39_TM=60.153
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=41.667
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=703
PRIMER_PAIR_40_PENALTY=4.626004
PRIMER_LEFT_40_PENALTY=2.830594
PRIMER_RIGHT_40_PENALTY=1.795410
PRIMER_LEFT_40_SEQUENCE=TGCTTTCTCTCGTTGTTCACTT
PRIMER_RIGHT_40_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_40=806,22
PRIMER_RIGHT_40=1522,21
PRIMER_LEFT_40_TM=59.169
PRIMER_RIGHT_40_TM=59.205
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=717
PRIMER_PAIR_41_PENALTY=4.655556
PRIMER_LEFT_41_PENALTY=1.103105
PRIMER_RIGHT_41_PENALTY=3.552451
PRIMER_LEFT_41_SEQUENCE=TGTTCACTTTCATTTGGCCTC
PRIMER_RIGHT_41_SEQUENCE=AGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_41=819,21
PRIMER_RIGHT_41=1523,21
PRIMER_LEFT_41_TM=60.103
PRIMER_RIGHT_41_TM=57.448
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=705
PRIMER_PAIR_42_PENALTY=4.667265
PRIMER_LEFT_42_PENALTY=1.017688
PRIMER_RIGHT_42_PENALTY=3.649576
PRIMER_LEFT_42_SEQUENCE=CAATGGAAACCCTAACAAGCA
PRIMER_RIGHT_42_SEQUENCE=GAAAAAGACCTCAAACGTCC
PRIMER_LEFT_42=740,21
PRIMER_RIGHT_42=1522,20
PRIMER_LEFT_42_TM=59.982
PRIMER_RIGHT_42_TM=56.350
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=783
PRIMER_PAIR_43_PENALTY=4.691835
PRIMER_LEFT_43_PENALTY=1.496015
PRIMER_RIGHT_43_PENALTY=3.195819
PRIMER_LEFT_43_SEQUENCE=ATTTGGCCTCTTGAACCATT
PRIMER_RIGHT_43_SEQUENCE=AAGAAAAAGACCTCAAACGTCC
PRIMER_LEFT_43=830,20
PRIMER_RIGHT_43=1524,22
PRIMER_LEFT_43_TM=58.504
PRIMER_RIGHT_43_TM=58.804
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=695
PRIMER_PAIR_44_PENALTY=4.713793
PRIMER_LEFT_44_PENALTY=0.051345
PRIMER_RIGHT_44_PENALTY=4.662448
PRIMER_LEFT_44_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_44_SEQUENCE=TCTCAGTCCCTTAAGAAAAAGACC
PRIMER_LEFT_44=827,20
PRIMER_RIGHT_44=1536,24
PRIMER_LEFT_44_TM=60.051
PRIMER_RIGHT_44_TM=59.338
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=710
PRIMER_PAIR_45_PENALTY=4.730400
PRIMER_LEFT_45_PENALTY=0.051345
PRIMER_RIGHT_45_PENALTY=4.679055
PRIMER_LEFT_45_SEQUENCE=TTCATTTGGCCTCTTGAACC
PRIMER_RIGHT_45_SEQUENCE=CGATATGAAATTCTCAGTCCCT
PRIMER_LEFT_45=827,20
PRIMER_RIGHT_45=1547,22
PRIMER_LEFT_45_TM=60.051
PRIMER_RIGHT_45_TM=57.321
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=721
PRIMER_PAIR_46_PENALTY=4.750941
PRIMER_LEFT_46_PENALTY=2.606973
PRIMER_RIGHT_46_PENALTY=2.143968
PRIMER_LEFT_46_SEQUENCE=TCACTTTCATTTGGCCTCTT
PRIMER_RIGHT_46_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_46=822,20
PRIMER_RIGHT_46=1523,22
PRIMER_LEFT_46_TM=57.393
PRIMER_RIGHT_46_TM=60.144
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=702
PRIMER_PAIR_47_PENALTY=4.750941
PRIMER_LEFT_47_PENALTY=2.606973
PRIMER_RIGHT_47_PENALTY=2.143968
PRIMER_LEFT_47_SEQUENCE=TTCACTTTCATTTGGCCTCT
PRIMER_RIGHT_47_SEQUENCE=AGAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_47=821,20
PRIMER_RIGHT_47=1523,22
PRIMER_LEFT_47_TM=57.393
PRIMER_RIGHT_47_TM=60.144
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=703
PRIMER_PAIR_48_PENALTY=4.752681
PRIMER_LEFT_48_PENALTY=1.103105
PRIMER_RIGHT_48_PENALTY=3.649576
PRIMER_LEFT_48_SEQUENCE=TGTTCACTTTCATTTGGCCTC
PRIMER_RIGHT_48_SEQUENCE=GAAAAAGACCTCAAACGTCC
PRIMER_LEFT_48=819,21
PRIMER_RIGHT_48=1522,20
PRIMER_LEFT_48_TM=60.103
PRIMER_RIGHT_48_TM=56.350
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=704
PRIMER_PAIR_49_PENALTY=4.809916
PRIMER_LEFT_49_PENALTY=3.014506
PRIMER_RIGHT_49_PENALTY=1.795410
PRIMER_LEFT_49_SEQUENCE=TGTTCACTTTCATTTGGCCTCT
PRIMER_RIGHT_49_SEQUENCE=GAAAAAGACCTCAAACGTCCA
PRIMER_LEFT_49=819,22
PRIMER_RIGHT_49=1522,21
PRIMER_LEFT_49_TM=61.015
PRIMER_RIGHT_49_TM=59.205
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=704
=
SEQUENCE_ID=MAGIC.329_3_12707506_12708054_ct-1_ws-0_13246_2996_TRUE
SEQUENCE_TARGET=950,648
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_12151771_12151786_mt-0_wil-2_186764_36354_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=ACAGTTGTTACANTCGTCNGANGAAATATCTGGCGTGCATTGCATCAATGCGTAGATATTCTGGAATTTTGTCAAGTTCGCAAAATCAACTCTGTAATGGNTATCANTGGACGATGGTGTGCTTCTTGCTGCGGAGGCTGCAGTAATCNTACGAGATGTTAAGTCTTCCCATATNNTCTTGAACTCTGTTAGATNTGAGNCGATAGTTGCNGTGTTCAANACCAAATACTGCGGNTCAATCTCCCAAAAAGCTGCAGATCCTGAGAAAGAAATGTTGGAGTAGCGGACAAGGCACAGNGTTGGATCAAGTNCCCAATANTACGCGTCNGTCTGGTTNACACAATCNTGTATCAACCAGCCTGCNGCGCCCTTNATACANTTAGAACANTCACTTGGAGTTGANNTNGGGATGCACATCCCTAATGCGTAGACTCGATCTTGCTCTTCTCCAATNGAACCGTAGTNGAAACCATCTNGAGANGCGGTATTGTTAGGAAGAGAAGAGANGATAAGACGANGATTTGANTCGTATGTACCGTTGGGTGTGAAATATTTACGGTTCTCTATGCATGTTTGTGCTGAAACAGAAGCAACACCANAGGCTAGAAGNANAAAACAGAGGATTGATAANAAACTCCTCTGCTTCANNTTTTGCCTNAGAGATTATAGCTTTGTTTTAGTGTTTNAACATCTGTTCGTTCCTTAGCAATTTAAACAGCAATTGTTGTTTCTCAGAAACTTTCTAAATGTCACAAAACAAAAGATGAAAATCAAAAGTCTTATTTCATTTTTTTCTGTTCATACAGTATTATTATGTTTAAATCCTTTCAAATAATAATNTAGATATNTACTAGGTNAGNAGGCTNGGGTCGAGGTACCANNNNNNNTTAACACTCATCCACGGCCANATTACGTAGCNTTTTCCTAANCAATTTCTTACCTTTAAAACNAAAACGCNTTCAAAANACGTCGCANTTGNNNCNNNNNAAANNCCNTAAAANNNNNNNNNNNNCCANCAGTTCCTGANGAGTCCTTANNNTTTCGTGAGCTCTAAGTTANCNTAGNTNCTATTATAACCTANNGCACTATNTCCATTCGCTAATAAGACGTNNNTAATGAACTATTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAAAAGATTATCCCTNNNTTNTTTTTANNCGCTNATTCNTTGTCTTTTGTTTACGCGTTTTTNATNGCTTACTAAAAAAGAAATCNAGTTTCTCNGAAAATNTNTAAATNNTGAATTCGTCAAACNGATAACAGTGGTCCTTNAACCCTCCNTGTTNGACATNTACGGCGTGAAACGGNCCCGCGACAAAAGTCAATGGTTTTNGGCTANTGGAAAACGTTGNATTTCATCATCATTCCTNACCATATGTNTTAACCGTTGANCCCANAAACNNNNNNNTCTCCTNTCACCAAACCTGACGNAGCAAAGGCAGATGCAGANGATCAGATGATCNTTGCAGNGGGCTATGTANNNNNNNNNNGGTTTTGCANATTAACCGGTTTNGTAGTATATCCTTNTNTCATTGGTTTTGNAAAAAAGATTCANTTACTTGTGTAGCACGTTNNCNNNNNNNNNNNNNTATCNCATANTCCCATGTCAAACCAAACCCAAAAANAAACTGAANTGTACACCATTTTANNNNNNTACNAANCATATCCTAGTCACACNAGNTTGAAGAGATTATAGGGTTTATAGAAGCNTCACCATGGCATANACTCNGANNATTTGGTAGTGNTNACACAATATTGTANAGAAACANAAANGGCTCTTGAGATTANNNNNTTAAAATAAAATAAAAAGGCTTTGTATNTAGCGATCATGAACCGTGCTAGAAATAGCCCGGCTAGAATCGTACANGTGACNAGTACTTCGCACAAGCGGATCGAGGTTAAAGCACAAAACTCACCCCCCCTACCTGTCCATGCTCAGCACTGTCCCTTNCAATCTCGATATCCNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_13901105_13901375_tsu-0_wil-2_2208013_54584_TRUE
SEQUENCE_TARGET=950,370
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANGNATGTGGTGANCANTACAAGGCTNAGAATCAANGGGAGANAACTCATNAGGAGANGATATTGGAGTTAATAGATGGAAGCAAACAAANATGGTTATGCNGAGATGGATCTTGCTAAAGTATTNAAGGAGGTGTNCTATACTAGTTGAAGAAGCTCGTTCAAGAAATGNTGTGTGCNCAAACAACTGNGAAGAGGATATCTCAAGAAGCAAAATTGATGAAGTCATTGATCCATGAAGCNTTGTGGAAAAGTAATCAAGGAGGCTTTGNAAAGAAGGTACCATGAAGTTTAANNAGAGANGGAGTTAGATCTACTNCAAGTCCATGGTNGATGATTNGGTGAAGCAAGTCGAGTAAGTGNCCAAGCCANNNNNNNNNNNNAAGATGAGTTCTCANNNNATTCAGTCNNTGTAGTCATTGGTCTACCCACNANNNNNNNNNNNNNNNNNNNNNNNNAGAAGAGCNNAAGNCACAAGTAAGTTGTGTTATACCNTAGGAATAGATTACCCTAACCTNTATGACCTTATNGGTNTCNGCNCAGGTACGNNNGGNTTCTAACTATTNTTNNNNNNGTGTGNTTGATGCTTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTGTTNGTANATTGCTCGCTNTAANNTTTATTATTGCTTGCATTNGTTGNGNNTGCATNATTTANAATAATATTGCTTGCATTAGTTNNTTAAGCGCAACTAGGCATGTGGANTGNTAAACCCATGCTTGTNTTGTTGCCTTAAGTATTTTNAGTATGTGTNGGTANAAAAGTAGGGTTGGTTTCTAGTATTTTTAGGAGCCTTAAGAACTGTAAATAGTNGAACNTAGNATTTTAGATNAACCCTAGAAGTTTAGGTNGGAACACTTTACNCGGTTGATCTTGTGGTGTTTTATGGGCGTGTTGGGGAAAAAGCAAGGGTTGGTGTGTCNNGTAAGTAAGNTGTTATGTGATAGGGAATCACATANGTAAGAATGAAACTTTAGTTGTGNCGTGTGANATCCGANCCNTAGATCANCAAGNNTGTCAAGGCNTCATGTGAGANAGGAAATCACANNTTNGAAGAGGAATAAGATANNCACTNTANAGAAGAGAGAGCCGCTGCCAAACCTANTANGGNAACAGCNCTAGTNTGGTTNATATTGCCAATAGCCATGAAGGAAGNACCNTTCACCNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTAAAGGGAATGAANGGANNNNGAGAGAGAGAGTNNNGTAGNTATTTTANTCATGAGNAGACGCCAAGANAGAGTGTGCAGACTATTCNNGTAAGGACGAAGGGGATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGAGNANACTTTCAGGTGATGGTGTNNNNNNNGAAGAAGATGTTGNTTNANCCAAACCGTNAGATTTAAGAAGANTTGTGTGGTAATGAAGTAAGGAAGAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAAGAAGAGGGANGNANCNCAAGACAAAGCCCTAGTTATCNGATGGAAAGGATAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=18
PRIMER_RIGHT_NUM_RETURNED=18
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=18
PRIMER_PAIR_0_PENALTY=7.259946
PRIMER_LEFT_0_PENALTY=2.947097
PRIMER_RIGHT_0_PENALTY=4.312850
PRIMER_LEFT_0_SEQUENCE=GGTGAAGCAAGTCGAGTAAGTG
PRIMER_RIGHT_0_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_0=568,22
PRIMER_RIGHT_0=1357,20
PRIMER_LEFT_0_TM=59.053
PRIMER_RIGHT_0_TM=64.313
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=790
PRIMER_PAIR_1_PENALTY=7.691377
PRIMER_LEFT_1_PENALTY=2.947097
PRIMER_RIGHT_1_PENALTY=4.744280
PRIMER_LEFT_1_SEQUENCE=GGTGAAGCAAGTCGAGTAAGTG
PRIMER_RIGHT_1_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_1=568,22
PRIMER_RIGHT_1=1356,20
PRIMER_LEFT_1_TM=59.053
PRIMER_RIGHT_1_TM=64.744
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=789
PRIMER_PAIR_2_PENALTY=8.049050
PRIMER_LEFT_2_PENALTY=3.736200
PRIMER_RIGHT_2_PENALTY=4.312850
PRIMER_LEFT_2_SEQUENCE=GAAGAAGCTCGTTCAAGAAATG
PRIMER_RIGHT_2_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_2=377,22
PRIMER_RIGHT_2=1357,20
PRIMER_LEFT_2_TM=58.264
PRIMER_RIGHT_2_TM=64.313
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=981
PRIMER_PAIR_3_PENALTY=8.074508
PRIMER_LEFT_3_PENALTY=3.761659
PRIMER_RIGHT_3_PENALTY=4.312850
PRIMER_LEFT_3_SEQUENCE=TGTGGAAAAGTAATCAAGGAGG
PRIMER_RIGHT_3_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_3=472,22
PRIMER_RIGHT_3=1357,20
PRIMER_LEFT_3_TM=58.238
PRIMER_RIGHT_3_TM=64.313
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=886
PRIMER_PAIR_4_PENALTY=8.480480
PRIMER_LEFT_4_PENALTY=3.736200
PRIMER_RIGHT_4_PENALTY=4.744280
PRIMER_LEFT_4_SEQUENCE=GAAGAAGCTCGTTCAAGAAATG
PRIMER_RIGHT_4_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_4=377,22
PRIMER_RIGHT_4=1356,20
PRIMER_LEFT_4_TM=58.264
PRIMER_RIGHT_4_TM=64.744
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=980
PRIMER_PAIR_5_PENALTY=8.505939
PRIMER_LEFT_5_PENALTY=3.761659
PRIMER_RIGHT_5_PENALTY=4.744280
PRIMER_LEFT_5_SEQUENCE=TGTGGAAAAGTAATCAAGGAGG
PRIMER_RIGHT_5_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_5=472,22
PRIMER_RIGHT_5=1356,20
PRIMER_LEFT_5_TM=58.238
PRIMER_RIGHT_5_TM=64.744
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=885
PRIMER_PAIR_6_PENALTY=9.096985
PRIMER_LEFT_6_PENALTY=4.784136
PRIMER_RIGHT_6_PENALTY=4.312850
PRIMER_LEFT_6_SEQUENCE=GGTGAAGCAAGTCGAGTAAGT
PRIMER_RIGHT_6_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_6=568,21
PRIMER_RIGHT_6=1357,20
PRIMER_LEFT_6_TM=56.216
PRIMER_RIGHT_6_TM=64.313
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=790
PRIMER_PAIR_7_PENALTY=9.120846
PRIMER_LEFT_7_PENALTY=4.807996
PRIMER_RIGHT_7_PENALTY=4.312850
PRIMER_LEFT_7_SEQUENCE=GGTGAAGCAAGTCGAGTAAG
PRIMER_RIGHT_7_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_7=568,20
PRIMER_RIGHT_7=1357,20
PRIMER_LEFT_7_TM=55.192
PRIMER_RIGHT_7_TM=64.313
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=60.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=790
PRIMER_PAIR_8_PENALTY=9.528415
PRIMER_LEFT_8_PENALTY=4.784136
PRIMER_RIGHT_8_PENALTY=4.744280
PRIMER_LEFT_8_SEQUENCE=GGTGAAGCAAGTCGAGTAAGT
PRIMER_RIGHT_8_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_8=568,21
PRIMER_RIGHT_8=1356,20
PRIMER_LEFT_8_TM=56.216
PRIMER_RIGHT_8_TM=64.744
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=5.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=789
PRIMER_PAIR_9_PENALTY=9.552276
PRIMER_LEFT_9_PENALTY=4.807996
PRIMER_RIGHT_9_PENALTY=4.744280
PRIMER_LEFT_9_SEQUENCE=GGTGAAGCAAGTCGAGTAAG
PRIMER_RIGHT_9_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_9=568,20
PRIMER_RIGHT_9=1356,20
PRIMER_LEFT_9_TM=55.192
PRIMER_RIGHT_9_TM=64.744
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=789
PRIMER_PAIR_10_PENALTY=9.839207
PRIMER_LEFT_10_PENALTY=5.526357
PRIMER_RIGHT_10_PENALTY=4.312850
PRIMER_LEFT_10_SEQUENCE=GTGGAAAAGTAATCAAGGAGG
PRIMER_RIGHT_10_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_10=473,21
PRIMER_RIGHT_10=1357,20
PRIMER_LEFT_10_TM=55.474
PRIMER_RIGHT_10_TM=64.313
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=60.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=885
PRIMER_PAIR_11_PENALTY=10.025530
PRIMER_LEFT_11_PENALTY=5.712680
PRIMER_RIGHT_11_PENALTY=4.312850
PRIMER_LEFT_11_SEQUENCE=GTGAAGCAAGTCGAGTAAGTG
PRIMER_RIGHT_11_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_11=569,21
PRIMER_RIGHT_11=1357,20
PRIMER_LEFT_11_TM=55.287
PRIMER_RIGHT_11_TM=64.313
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=60.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=789
PRIMER_PAIR_12_PENALTY=10.270637
PRIMER_LEFT_12_PENALTY=5.526357
PRIMER_RIGHT_12_PENALTY=4.744280
PRIMER_LEFT_12_SEQUENCE=GTGGAAAAGTAATCAAGGAGG
PRIMER_RIGHT_12_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_12=473,21
PRIMER_RIGHT_12=1356,20
PRIMER_LEFT_12_TM=55.474
PRIMER_RIGHT_12_TM=64.744
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=884
PRIMER_PAIR_13_PENALTY=10.456960
PRIMER_LEFT_13_PENALTY=5.712680
PRIMER_RIGHT_13_PENALTY=4.744280
PRIMER_LEFT_13_SEQUENCE=GTGAAGCAAGTCGAGTAAGTG
PRIMER_RIGHT_13_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_13=569,21
PRIMER_RIGHT_13=1356,20
PRIMER_LEFT_13_TM=55.287
PRIMER_RIGHT_13_TM=64.744
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=788
PRIMER_PAIR_14_PENALTY=10.871179
PRIMER_LEFT_14_PENALTY=6.558329
PRIMER_RIGHT_14_PENALTY=4.312850
PRIMER_LEFT_14_SEQUENCE=GAAGAGGATATCTCAAGAAGCA
PRIMER_RIGHT_14_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_14=419,22
PRIMER_RIGHT_14=1357,20
PRIMER_LEFT_14_TM=55.442
PRIMER_RIGHT_14_TM=64.313
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=60.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=939
PRIMER_PAIR_15_PENALTY=11.302609
PRIMER_LEFT_15_PENALTY=6.558329
PRIMER_RIGHT_15_PENALTY=4.744280
PRIMER_LEFT_15_SEQUENCE=GAAGAGGATATCTCAAGAAGCA
PRIMER_RIGHT_15_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_15=419,22
PRIMER_RIGHT_15=1356,20
PRIMER_LEFT_15_TM=55.442
PRIMER_RIGHT_15_TM=64.744
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=938
PRIMER_PAIR_16_PENALTY=11.474261
PRIMER_LEFT_16_PENALTY=7.161411
PRIMER_RIGHT_16_PENALTY=4.312850
PRIMER_LEFT_16_SEQUENCE=GTGGAAAAGTAATCAAGGAGGCTTT
PRIMER_RIGHT_16_SEQUENCE=GTTTGGCAGCGGCTCTCTCT
PRIMER_LEFT_16=473,25
PRIMER_RIGHT_16=1357,20
PRIMER_LEFT_16_TM=62.161
PRIMER_RIGHT_16_TM=64.313
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=60.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=885
PRIMER_PAIR_17_PENALTY=11.905691
PRIMER_LEFT_17_PENALTY=7.161411
PRIMER_RIGHT_17_PENALTY=4.744280
PRIMER_LEFT_17_SEQUENCE=GTGGAAAAGTAATCAAGGAGGCTTT
PRIMER_RIGHT_17_SEQUENCE=TTTGGCAGCGGCTCTCTCTT
PRIMER_LEFT_17=473,25
PRIMER_RIGHT_17=1356,20
PRIMER_LEFT_17_TM=62.161
PRIMER_RIGHT_17_TM=64.744
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=884
=
SEQUENCE_ID=MAGIC.446_5_18232626_18232890_po-0_rsch-4_8393_39591_TRUE
SEQUENCE_TARGET=950,364
SEQUENCE_TEMPLATE=TTTGTCAANCATAGCTATCTNTTTACATAATTACAGNAGATAAGACTANTGGTGGCTAAGAGTAAAGNACAAAAACCATTCTTCCAAACAACCTGTAATAATGCATTCTGANNAGAACTGTCACATNTATGTCCCAGTAAGTAGATGTTAGCTGCTCTCTTTGGCATAGGGTCGGGGCNGGGGCTTGTCTGACCAATACTGTCTATCTGTCTTCTTCAACAAAGAAAGCATTTCAAGTTGCATNATATGTTAAGTGTGCCTTTCCAACNTCGTAAAGCAATAGNAGAATATTGTACCAACGAAGGACTGNGCCTTTCCNATTTNTCACTCTGTTCAGTCCAGTCTAAAATTCACTTCACTGAGTTCAGTGGCNTTTCAACATTACGGAGAGCCTNTNTTCTTTCAGCTTTCTCGAAGTCCTTCCTTGTTCGTTTGTTGGCATCTATATCTATACTGATGAGTAGAGAGGTNCAATCAAATNCACAGGCGGAAATACTTCACTATGCATCGACANATTCTNTGATCCTCTCCATCGCACTGAGAANTTCCAGGTTTCACTGGTTGNGAGCTAAGCAATGAGAAAATGTTATAGATCANCAGACATCTGCACTAGATTGAAGATAACGTTATATCATTGAACGGACCAGTTAATCAGGTAGATGCTTTCTTTATATTTTCCCANGTAGNTGGACCTTCCNGGTGGGTATTNACAAAGTATGNCACAATAAGGGTATTAGAAATACNGACAANAGTGCCTAGTGTCAGCCTCGGTATTACTGCATCTCTTTCCCTCAACCAAATGTACATTGTGAATCTCTGATGNATTCTCTTCATACTATAACACAAATATGTTGGGGACTCTATCACGGATTCTATTGGATAAACNAAATAATCTNTTAGCATAATGATCTTCTTTTCTATGATANCTTTCGGCCGGTTAAGTATATGAGGCGCCCGCTTAACAAGCTGNCTAACCACATTATAGTCCAAACCAGCCTTCNCAAAGCAATCAAATCTCTCCTGTAGCTCATCTCCCCTCCCNTGGAACTTTTTCAGAGCCCTCACCATCTCATCGGAATTCTCTACATACCCTAACTTCAACAAGAATGTCGTCTTCTCTGCATTNCTCNAATCCAGTGAATCAAGTTCAATTCTACCTTTATTAGTTTTAGAAGCTATACTGAACAATNTCAAAGGCTCTTCCTTAATGATCTGACATAACTCATCTCTTCNGATTTTNNATCTATTCCAAACAGCTCTAGGTNCTAGNAAAGAGGANGAACANATANGCTTCATGTGACACNGTAAAAACCTAACNANAAACTGCTTTTCNATTCTGATACATTTNAAGAATTTCAACGCCTNTAAAATTCTTTTTNCAGATTTCTCTAATANCANCTCGGNGTTGTCTATCANTAATTTATCNATTTCATTCATCTGAAGTCCAACTTTATGAAACTTTNCAAACATAATATAANCNTTATTGCCAGAANTTTCACCTACCAAATCTGGATANGCNTTTAAAAGANTACTCAACTTCTNCTCTTTAAACCCCACCTTNTCAAGGAGCTNCATTGTCTCAAGAATCCGGCGCCAGTTATATGTTTTTNTANCTGACAANTACCGNGCAAGCCAATCNCATCCTAAGTTTAATCCNTTCAACTTATTTACCACAGAAACAAANTCACNATCAATGCCACCAACCAGAAGCANCGGGCAACAAGTAACAAGCTTAATGACNATANNCTTNCTAAGAACCAANNTCTCATAAGNTTCAAGTTTCGATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.950077
PRIMER_LEFT_0_PENALTY=0.912701
PRIMER_RIGHT_0_PENALTY=2.037376
PRIMER_LEFT_0_SEQUENCE=TGTTGGGGACTCTATCACGG
PRIMER_RIGHT_0_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_0=849,20
PRIMER_RIGHT_0=1709,20
PRIMER_LEFT_0_TM=60.913
PRIMER_RIGHT_0_TM=62.037
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=861
PRIMER_PAIR_1_PENALTY=3.162080
PRIMER_LEFT_1_PENALTY=0.195358
PRIMER_RIGHT_1_PENALTY=2.966722
PRIMER_LEFT_1_SEQUENCE=GCATCTCTTTCCCTCAACCA
PRIMER_RIGHT_1_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_1=778,20
PRIMER_RIGHT_1=1733,20
PRIMER_LEFT_1_TM=60.195
PRIMER_RIGHT_1_TM=57.033
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=956
PRIMER_PAIR_2_PENALTY=3.162080
PRIMER_LEFT_2_PENALTY=0.195358
PRIMER_RIGHT_2_PENALTY=2.966722
PRIMER_LEFT_2_SEQUENCE=TGCATCTCTTTCCCTCAACC
PRIMER_RIGHT_2_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_2=777,20
PRIMER_RIGHT_2=1733,20
PRIMER_LEFT_2_TM=60.195
PRIMER_RIGHT_2_TM=57.033
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=957
PRIMER_PAIR_3_PENALTY=3.293238
PRIMER_LEFT_3_PENALTY=1.255861
PRIMER_RIGHT_3_PENALTY=2.037376
PRIMER_LEFT_3_SEQUENCE=CAGCCTCGGTATTACTGCATC
PRIMER_RIGHT_3_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_3=762,21
PRIMER_RIGHT_3=1709,20
PRIMER_LEFT_3_TM=59.744
PRIMER_RIGHT_3_TM=62.037
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=948
PRIMER_PAIR_4_PENALTY=3.626873
PRIMER_LEFT_4_PENALTY=1.589496
PRIMER_RIGHT_4_PENALTY=2.037376
PRIMER_LEFT_4_SEQUENCE=GGGGACTCTATCACGGATTCT
PRIMER_RIGHT_4_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_4=853,21
PRIMER_RIGHT_4=1709,20
PRIMER_LEFT_4_TM=59.411
PRIMER_RIGHT_4_TM=62.037
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=857
PRIMER_PAIR_5_PENALTY=3.691717
PRIMER_LEFT_5_PENALTY=0.195358
PRIMER_RIGHT_5_PENALTY=3.496360
PRIMER_LEFT_5_SEQUENCE=GCATCTCTTTCCCTCAACCA
PRIMER_RIGHT_5_SEQUENCE=TTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_5=778,20
PRIMER_RIGHT_5=1735,22
PRIMER_LEFT_5_TM=60.195
PRIMER_RIGHT_5_TM=58.504
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=11.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=958
PRIMER_PAIR_6_PENALTY=3.691717
PRIMER_LEFT_6_PENALTY=0.195358
PRIMER_RIGHT_6_PENALTY=3.496360
PRIMER_LEFT_6_SEQUENCE=TGCATCTCTTTCCCTCAACC
PRIMER_RIGHT_6_SEQUENCE=TTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_6=777,20
PRIMER_RIGHT_6=1735,22
PRIMER_LEFT_6_TM=60.195
PRIMER_RIGHT_6_TM=58.504
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=11.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=959
PRIMER_PAIR_7_PENALTY=3.879423
PRIMER_LEFT_7_PENALTY=0.912701
PRIMER_RIGHT_7_PENALTY=2.966722
PRIMER_LEFT_7_SEQUENCE=TGTTGGGGACTCTATCACGG
PRIMER_RIGHT_7_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_7=849,20
PRIMER_RIGHT_7=1733,20
PRIMER_LEFT_7_TM=60.913
PRIMER_RIGHT_7_TM=57.033
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.9000
PRIMER_RIGHT_7_END_STABILITY=11.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=885
PRIMER_PAIR_8_PENALTY=3.926762
PRIMER_LEFT_8_PENALTY=1.889386
PRIMER_RIGHT_8_PENALTY=2.037376
PRIMER_LEFT_8_SEQUENCE=TCGGCCGGTTAAGTATATGAG
PRIMER_RIGHT_8_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_8=929,21
PRIMER_RIGHT_8=1709,20
PRIMER_LEFT_8_TM=59.111
PRIMER_RIGHT_8_TM=62.037
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=781
PRIMER_PAIR_9_PENALTY=4.042800
PRIMER_LEFT_9_PENALTY=0.195358
PRIMER_RIGHT_9_PENALTY=3.847442
PRIMER_LEFT_9_SEQUENCE=GCATCTCTTTCCCTCAACCA
PRIMER_RIGHT_9_SEQUENCE=TAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_9=778,20
PRIMER_RIGHT_9=1734,21
PRIMER_LEFT_9_TM=60.195
PRIMER_RIGHT_9_TM=57.153
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=11.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=957
PRIMER_PAIR_10_PENALTY=4.042800
PRIMER_LEFT_10_PENALTY=0.195358
PRIMER_RIGHT_10_PENALTY=3.847442
PRIMER_LEFT_10_SEQUENCE=TGCATCTCTTTCCCTCAACC
PRIMER_RIGHT_10_SEQUENCE=TAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_10=777,20
PRIMER_RIGHT_10=1734,21
PRIMER_LEFT_10_TM=60.195
PRIMER_RIGHT_10_TM=57.153
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=11.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=958
PRIMER_PAIR_11_PENALTY=4.069874
PRIMER_LEFT_11_PENALTY=0.195358
PRIMER_RIGHT_11_PENALTY=3.874516
PRIMER_LEFT_11_SEQUENCE=TGCATCTCTTTCCCTCAACC
PRIMER_RIGHT_11_SEQUENCE=TGCTTCTGGTTGGTGGCATT
PRIMER_LEFT_11=777,20
PRIMER_RIGHT_11=1711,20
PRIMER_LEFT_11_TM=60.195
PRIMER_RIGHT_11_TM=63.875
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=935
PRIMER_PAIR_12_PENALTY=4.175428
PRIMER_LEFT_12_PENALTY=2.138051
PRIMER_RIGHT_12_PENALTY=2.037376
PRIMER_LEFT_12_SEQUENCE=ATGTTGGGGACTCTATCACGG
PRIMER_RIGHT_12_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_12=848,21
PRIMER_RIGHT_12=1709,20
PRIMER_LEFT_12_TM=61.138
PRIMER_RIGHT_12_TM=62.037
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=862
PRIMER_PAIR_13_PENALTY=4.209811
PRIMER_LEFT_13_PENALTY=2.172434
PRIMER_RIGHT_13_PENALTY=2.037376
PRIMER_LEFT_13_SEQUENCE=GCCTCGGTATTACTGCATCTC
PRIMER_RIGHT_13_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_13=764,21
PRIMER_RIGHT_13=1709,20
PRIMER_LEFT_13_TM=58.828
PRIMER_RIGHT_13_TM=62.037
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.3000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=946
PRIMER_PAIR_14_PENALTY=4.222583
PRIMER_LEFT_14_PENALTY=1.255861
PRIMER_RIGHT_14_PENALTY=2.966722
PRIMER_LEFT_14_SEQUENCE=CAGCCTCGGTATTACTGCATC
PRIMER_RIGHT_14_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_14=762,21
PRIMER_RIGHT_14=1733,20
PRIMER_LEFT_14_TM=59.744
PRIMER_RIGHT_14_TM=57.033
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=11.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=972
PRIMER_PAIR_15_PENALTY=4.263756
PRIMER_LEFT_15_PENALTY=2.226380
PRIMER_RIGHT_15_PENALTY=2.037376
PRIMER_LEFT_15_SEQUENCE=GCCTCGGTATTACTGCATCTCT
PRIMER_RIGHT_15_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_15=764,22
PRIMER_RIGHT_15=1709,20
PRIMER_LEFT_15_TM=59.774
PRIMER_RIGHT_15_TM=62.037
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=946
PRIMER_PAIR_16_PENALTY=4.263756
PRIMER_LEFT_16_PENALTY=2.226380
PRIMER_RIGHT_16_PENALTY=2.037376
PRIMER_LEFT_16_SEQUENCE=AGCCTCGGTATTACTGCATCTC
PRIMER_RIGHT_16_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_16=763,22
PRIMER_RIGHT_16=1709,20
PRIMER_LEFT_16_TM=59.774
PRIMER_RIGHT_16_TM=62.037
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.3000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=947
PRIMER_PAIR_17_PENALTY=4.373461
PRIMER_LEFT_17_PENALTY=2.336085
PRIMER_RIGHT_17_PENALTY=2.037376
PRIMER_LEFT_17_SEQUENCE=TTCGGCCGGTTAAGTATATGAG
PRIMER_RIGHT_17_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_17=928,22
PRIMER_RIGHT_17=1709,20
PRIMER_LEFT_17_TM=60.336
PRIMER_RIGHT_17_TM=62.037
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=782
PRIMER_PAIR_18_PENALTY=4.409060
PRIMER_LEFT_18_PENALTY=0.912701
PRIMER_RIGHT_18_PENALTY=3.496360
PRIMER_LEFT_18_SEQUENCE=TGTTGGGGACTCTATCACGG
PRIMER_RIGHT_18_SEQUENCE=TTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_18=849,20
PRIMER_RIGHT_18=1735,22
PRIMER_LEFT_18_TM=60.913
PRIMER_RIGHT_18_TM=58.504
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=11.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=887
PRIMER_PAIR_19_PENALTY=4.556218
PRIMER_LEFT_19_PENALTY=1.589496
PRIMER_RIGHT_19_PENALTY=2.966722
PRIMER_LEFT_19_SEQUENCE=GGGGACTCTATCACGGATTCT
PRIMER_RIGHT_19_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_19=853,21
PRIMER_RIGHT_19=1733,20
PRIMER_LEFT_19_TM=59.411
PRIMER_RIGHT_19_TM=57.033
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=11.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=881
PRIMER_PAIR_20_PENALTY=4.585883
PRIMER_LEFT_20_PENALTY=2.548507
PRIMER_RIGHT_20_PENALTY=2.037376
PRIMER_LEFT_20_SEQUENCE=CTGCATCTCTTTCCCTCAAC
PRIMER_RIGHT_20_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_20=776,20
PRIMER_RIGHT_20=1709,20
PRIMER_LEFT_20_TM=57.451
PRIMER_RIGHT_20_TM=62.037
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=934
PRIMER_PAIR_21_PENALTY=4.646572
PRIMER_LEFT_21_PENALTY=2.609196
PRIMER_RIGHT_21_PENALTY=2.037376
PRIMER_LEFT_21_SEQUENCE=ACTGCATCTCTTTCCCTCAAC
PRIMER_RIGHT_21_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_21=775,21
PRIMER_RIGHT_21=1709,20
PRIMER_LEFT_21_TM=58.391
PRIMER_RIGHT_21_TM=62.037
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=935
PRIMER_PAIR_22_PENALTY=4.680346
PRIMER_LEFT_22_PENALTY=2.642969
PRIMER_RIGHT_22_PENALTY=2.037376
PRIMER_LEFT_22_SEQUENCE=CCTAGTGTCAGCCTCGGTATT
PRIMER_RIGHT_22_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_22=754,21
PRIMER_RIGHT_22=1709,20
PRIMER_LEFT_22_TM=58.357
PRIMER_RIGHT_22_TM=62.037
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=5.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=956
PRIMER_PAIR_23_PENALTY=4.722911
PRIMER_LEFT_23_PENALTY=2.685535
PRIMER_RIGHT_23_PENALTY=2.037376
PRIMER_LEFT_23_SEQUENCE=CGGCCGGTTAAGTATATGAG
PRIMER_RIGHT_23_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_23=930,20
PRIMER_RIGHT_23=1709,20
PRIMER_LEFT_23_TM=57.314
PRIMER_RIGHT_23_TM=62.037
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=780
PRIMER_PAIR_24_PENALTY=4.752221
PRIMER_LEFT_24_PENALTY=1.255861
PRIMER_RIGHT_24_PENALTY=3.496360
PRIMER_LEFT_24_SEQUENCE=CAGCCTCGGTATTACTGCATC
PRIMER_RIGHT_24_SEQUENCE=TTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_24=762,21
PRIMER_RIGHT_24=1735,22
PRIMER_LEFT_24_TM=59.744
PRIMER_RIGHT_24_TM=58.504
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=11.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=974
PRIMER_PAIR_25_PENALTY=4.760143
PRIMER_LEFT_25_PENALTY=0.912701
PRIMER_RIGHT_25_PENALTY=3.847442
PRIMER_LEFT_25_SEQUENCE=TGTTGGGGACTCTATCACGG
PRIMER_RIGHT_25_SEQUENCE=TAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_25=849,20
PRIMER_RIGHT_25=1734,21
PRIMER_LEFT_25_TM=60.913
PRIMER_RIGHT_25_TM=57.153
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.9000
PRIMER_RIGHT_25_END_STABILITY=11.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=886
PRIMER_PAIR_26_PENALTY=4.787217
PRIMER_LEFT_26_PENALTY=0.912701
PRIMER_RIGHT_26_PENALTY=3.874516
PRIMER_LEFT_26_SEQUENCE=TGTTGGGGACTCTATCACGG
PRIMER_RIGHT_26_SEQUENCE=TGCTTCTGGTTGGTGGCATT
PRIMER_LEFT_26=849,20
PRIMER_RIGHT_26=1711,20
PRIMER_LEFT_26_TM=60.913
PRIMER_RIGHT_26_TM=63.875
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=863
PRIMER_PAIR_27_PENALTY=4.810221
PRIMER_LEFT_27_PENALTY=1.843499
PRIMER_RIGHT_27_PENALTY=2.966722
PRIMER_LEFT_27_SEQUENCE=CATCTCTTTCCCTCAACCAAA
PRIMER_RIGHT_27_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_27=779,21
PRIMER_RIGHT_27=1733,20
PRIMER_LEFT_27_TM=59.157
PRIMER_RIGHT_27_TM=57.033
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.8000
PRIMER_RIGHT_27_END_STABILITY=11.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=955
PRIMER_PAIR_28_PENALTY=4.856108
PRIMER_LEFT_28_PENALTY=1.889386
PRIMER_RIGHT_28_PENALTY=2.966722
PRIMER_LEFT_28_SEQUENCE=TCGGCCGGTTAAGTATATGAG
PRIMER_RIGHT_28_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_28=929,21
PRIMER_RIGHT_28=1733,20
PRIMER_LEFT_28_TM=59.111
PRIMER_RIGHT_28_TM=57.033
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=11.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=805
PRIMER_PAIR_29_PENALTY=5.085856
PRIMER_LEFT_29_PENALTY=1.589496
PRIMER_RIGHT_29_PENALTY=3.496360
PRIMER_LEFT_29_SEQUENCE=GGGGACTCTATCACGGATTCT
PRIMER_RIGHT_29_SEQUENCE=TTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_29=853,21
PRIMER_RIGHT_29=1735,22
PRIMER_LEFT_29_TM=59.411
PRIMER_RIGHT_29_TM=58.504
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=11.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=883
PRIMER_PAIR_30_PENALTY=5.102715
PRIMER_LEFT_30_PENALTY=3.065338
PRIMER_RIGHT_30_PENALTY=2.037376
PRIMER_LEFT_30_SEQUENCE=GCCTCGGTATTACTGCATCT
PRIMER_RIGHT_30_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_30=764,20
PRIMER_RIGHT_30=1709,20
PRIMER_LEFT_30_TM=56.935
PRIMER_RIGHT_30_TM=62.037
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=946
PRIMER_PAIR_31_PENALTY=5.102715
PRIMER_LEFT_31_PENALTY=3.065338
PRIMER_RIGHT_31_PENALTY=2.037376
PRIMER_LEFT_31_SEQUENCE=AGCCTCGGTATTACTGCATC
PRIMER_RIGHT_31_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_31=763,20
PRIMER_RIGHT_31=1709,20
PRIMER_LEFT_31_TM=56.935
PRIMER_RIGHT_31_TM=62.037
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=947
PRIMER_PAIR_32_PENALTY=5.103303
PRIMER_LEFT_32_PENALTY=1.255861
PRIMER_RIGHT_32_PENALTY=3.847442
PRIMER_LEFT_32_SEQUENCE=CAGCCTCGGTATTACTGCATC
PRIMER_RIGHT_32_SEQUENCE=TAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_32=762,21
PRIMER_RIGHT_32=1734,21
PRIMER_LEFT_32_TM=59.744
PRIMER_RIGHT_32_TM=57.153
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=11.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=973
PRIMER_PAIR_33_PENALTY=5.104773
PRIMER_LEFT_33_PENALTY=2.138051
PRIMER_RIGHT_33_PENALTY=2.966722
PRIMER_LEFT_33_SEQUENCE=ATGTTGGGGACTCTATCACGG
PRIMER_RIGHT_33_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_33=848,21
PRIMER_RIGHT_33=1733,20
PRIMER_LEFT_33_TM=61.138
PRIMER_RIGHT_33_TM=57.033
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.9000
PRIMER_RIGHT_33_END_STABILITY=11.2000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=886
PRIMER_PAIR_34_PENALTY=5.104773
PRIMER_LEFT_34_PENALTY=2.138051
PRIMER_RIGHT_34_PENALTY=2.966722
PRIMER_LEFT_34_SEQUENCE=CTGCATCTCTTTCCCTCAACC
PRIMER_RIGHT_34_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_34=776,21
PRIMER_RIGHT_34=1733,20
PRIMER_LEFT_34_TM=61.138
PRIMER_RIGHT_34_TM=57.033
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=11.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=958
PRIMER_PAIR_35_PENALTY=5.130377
PRIMER_LEFT_35_PENALTY=1.255861
PRIMER_RIGHT_35_PENALTY=3.874516
PRIMER_LEFT_35_SEQUENCE=CAGCCTCGGTATTACTGCATC
PRIMER_RIGHT_35_SEQUENCE=TGCTTCTGGTTGGTGGCATT
PRIMER_LEFT_35=762,21
PRIMER_RIGHT_35=1711,20
PRIMER_LEFT_35_TM=59.744
PRIMER_RIGHT_35_TM=63.875
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=8.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=950
PRIMER_PAIR_36_PENALTY=5.139156
PRIMER_LEFT_36_PENALTY=2.172434
PRIMER_RIGHT_36_PENALTY=2.966722
PRIMER_LEFT_36_SEQUENCE=GCCTCGGTATTACTGCATCTC
PRIMER_RIGHT_36_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_36=764,21
PRIMER_RIGHT_36=1733,20
PRIMER_LEFT_36_TM=58.828
PRIMER_RIGHT_36_TM=57.033
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.3000
PRIMER_RIGHT_36_END_STABILITY=11.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=970
PRIMER_PAIR_37_PENALTY=5.143958
PRIMER_LEFT_37_PENALTY=3.106581
PRIMER_RIGHT_37_PENALTY=2.037376
PRIMER_LEFT_37_SEQUENCE=TATGTTGGGGACTCTATCACGG
PRIMER_RIGHT_37_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_37=847,22
PRIMER_RIGHT_37=1709,20
PRIMER_LEFT_37_TM=61.107
PRIMER_RIGHT_37_TM=62.037
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=863
PRIMER_PAIR_38_PENALTY=5.193102
PRIMER_LEFT_38_PENALTY=2.226380
PRIMER_RIGHT_38_PENALTY=2.966722
PRIMER_LEFT_38_SEQUENCE=GCCTCGGTATTACTGCATCTCT
PRIMER_RIGHT_38_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_38=764,22
PRIMER_RIGHT_38=1733,20
PRIMER_LEFT_38_TM=59.774
PRIMER_RIGHT_38_TM=57.033
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=11.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=970
PRIMER_PAIR_39_PENALTY=5.193102
PRIMER_LEFT_39_PENALTY=2.226380
PRIMER_RIGHT_39_PENALTY=2.966722
PRIMER_LEFT_39_SEQUENCE=AGCCTCGGTATTACTGCATCTC
PRIMER_RIGHT_39_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_39=763,22
PRIMER_RIGHT_39=1733,20
PRIMER_LEFT_39_TM=59.774
PRIMER_RIGHT_39_TM=57.033
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.3000
PRIMER_RIGHT_39_END_STABILITY=11.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=971
PRIMER_PAIR_40_PENALTY=5.257068
PRIMER_LEFT_40_PENALTY=2.290346
PRIMER_RIGHT_40_PENALTY=2.966722
PRIMER_LEFT_40_SEQUENCE=CATCTCTTTCCCTCAACCAA
PRIMER_RIGHT_40_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_40=779,20
PRIMER_RIGHT_40=1733,20
PRIMER_LEFT_40_TM=57.710
PRIMER_RIGHT_40_TM=57.033
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=11.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=955
PRIMER_PAIR_41_PENALTY=5.261677
PRIMER_LEFT_41_PENALTY=3.224301
PRIMER_RIGHT_41_PENALTY=2.037376
PRIMER_LEFT_41_SEQUENCE=TTACTGCATCTCTTTCCCTCAAC
PRIMER_RIGHT_41_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_41=773,23
PRIMER_RIGHT_41=1709,20
PRIMER_LEFT_41_TM=59.776
PRIMER_RIGHT_41_TM=62.037
PRIMER_LEFT_41_GC_PERCENT=43.478
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=937
PRIMER_PAIR_42_PENALTY=5.267104
PRIMER_LEFT_42_PENALTY=0.912701
PRIMER_RIGHT_42_PENALTY=4.354403
PRIMER_LEFT_42_SEQUENCE=TGTTGGGGACTCTATCACGG
PRIMER_RIGHT_42_SEQUENCE=TCCAGATTTGGTAGGTGAAA
PRIMER_LEFT_42=849,20
PRIMER_RIGHT_42=1512,20
PRIMER_LEFT_42_TM=60.913
PRIMER_RIGHT_42_TM=55.646
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=7.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=664
PRIMER_PAIR_43_PENALTY=5.302807
PRIMER_LEFT_43_PENALTY=2.336085
PRIMER_RIGHT_43_PENALTY=2.966722
PRIMER_LEFT_43_SEQUENCE=TTCGGCCGGTTAAGTATATGAG
PRIMER_RIGHT_43_SEQUENCE=AAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_43=928,22
PRIMER_RIGHT_43=1733,20
PRIMER_LEFT_43_TM=60.336
PRIMER_RIGHT_43_TM=57.033
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=11.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=806
PRIMER_PAIR_44_PENALTY=5.323767
PRIMER_LEFT_44_PENALTY=3.286391
PRIMER_RIGHT_44_PENALTY=2.037376
PRIMER_LEFT_44_SEQUENCE=TGCCTAGTGTCAGCCTCGGT
PRIMER_RIGHT_44_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_44=752,20
PRIMER_RIGHT_44=1709,20
PRIMER_LEFT_44_TM=63.286
PRIMER_RIGHT_44_TM=62.037
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=958
PRIMER_PAIR_45_PENALTY=5.339858
PRIMER_LEFT_45_PENALTY=1.843499
PRIMER_RIGHT_45_PENALTY=3.496360
PRIMER_LEFT_45_SEQUENCE=CATCTCTTTCCCTCAACCAAA
PRIMER_RIGHT_45_SEQUENCE=TTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_45=779,21
PRIMER_RIGHT_45=1735,22
PRIMER_LEFT_45_TM=59.157
PRIMER_RIGHT_45_TM=58.504
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.8000
PRIMER_RIGHT_45_END_STABILITY=11.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=957
PRIMER_PAIR_46_PENALTY=5.385745
PRIMER_LEFT_46_PENALTY=1.889386
PRIMER_RIGHT_46_PENALTY=3.496360
PRIMER_LEFT_46_SEQUENCE=TCGGCCGGTTAAGTATATGAG
PRIMER_RIGHT_46_SEQUENCE=TTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_46=929,21
PRIMER_RIGHT_46=1735,22
PRIMER_LEFT_46_TM=59.111
PRIMER_RIGHT_46_TM=58.504
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.6000
PRIMER_RIGHT_46_END_STABILITY=11.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=807
PRIMER_PAIR_47_PENALTY=5.386036
PRIMER_LEFT_47_PENALTY=0.195358
PRIMER_RIGHT_47_PENALTY=5.190679
PRIMER_LEFT_47_SEQUENCE=GCATCTCTTTCCCTCAACCA
PRIMER_RIGHT_47_SEQUENCE=CATTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_47=778,20
PRIMER_RIGHT_47=1737,24
PRIMER_LEFT_47_TM=60.195
PRIMER_RIGHT_47_TM=61.191
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=41.667
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=11.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=960
PRIMER_PAIR_48_PENALTY=5.386036
PRIMER_LEFT_48_PENALTY=0.195358
PRIMER_RIGHT_48_PENALTY=5.190679
PRIMER_LEFT_48_SEQUENCE=TGCATCTCTTTCCCTCAACC
PRIMER_RIGHT_48_SEQUENCE=CATTAAGCTTGTTACTTGTTGCCC
PRIMER_LEFT_48=777,20
PRIMER_RIGHT_48=1737,24
PRIMER_LEFT_48_TM=60.195
PRIMER_RIGHT_48_TM=61.191
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=41.667
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=11.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=961
PRIMER_PAIR_49_PENALTY=5.394235
PRIMER_LEFT_49_PENALTY=3.356859
PRIMER_RIGHT_49_PENALTY=2.037376
PRIMER_LEFT_49_SEQUENCE=TCGGTATTACTGCATCTCTTTCC
PRIMER_RIGHT_49_SEQUENCE=CTTCTGGTTGGTGGCATTGA
PRIMER_LEFT_49=767,23
PRIMER_RIGHT_49=1709,20
PRIMER_LEFT_49_TM=59.643
PRIMER_RIGHT_49_TM=62.037
PRIMER_LEFT_49_GC_PERCENT=43.478
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=943
=
SEQUENCE_ID=MAGIC.338_3_13803008_13803122_rsch-4_tsu-0_155504_88181_TRUE
SEQUENCE_TARGET=950,214
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_20150068_20150161_po-0_sf-2_49800_659335_TRUE
SEQUENCE_TARGET=950,193
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAATCCAATGTTTTTGTAGAGCGACCGGTCAATTAAGTGNTGGTAANAACTAAAAACATGTAATTAATGTGCTTAATNTGNAAATANTATTCNNCACCCAATTTATAACAATCGAGCTTATNTTAAAGCGATGATTAGCTGNNNNNNNNNNNTCTCAGTGACTAAGCAGAAGCAATNAATTAATGTATAGAAAAACTGAACTAAGAAAAGTAAAATANGCAAGTAAATNAATAAATAATGAGGAAATTAAACACTTGNCAAAACTCAACAAATGACTAAGAATACAAGNAAAACTTCGAGCATGATGACGATCAGTCGTAAGAAAACAAAACNNNNNNNNNNNNNTAATGTAGTATCCAACNAATGTTATGTAAGACATAGTTTCAAGGGTTTACCATTTTTGCTTAAGTNACTAAGTTCATANTTTAAACATTTTCTAGCTAACACCNGTTTGGTAGNTTAAGACANTCTAGTTTTTGTTGGAAATTAACATCATNTTAAAGGTATTTAAATATTTAAGGAGAGAGATCATATACGTTTCACCTCTNGAATTCNTTTTCATCTTTATAATATNNTATTGTATTTANTATNTATGGTTTCTNANNNTTTTNGAATATTTAATCANTTCNNATAANNAACGANNATACATTAGNNNTNGNTATAAAAAANCGNANGCAAATAGTTNATATTGNTTTGNCGGTTTTCTCGACATATTTNNACATATACCTTAGCCAAAATGGNCTTTTCATTTTCAAACAACCTTTTCTTTTCAAANCCTATTAATCCTCCTACTTCTTCATCNCCATATTTGAATCAAAACTATCTAACTTCTAATGTAATAATTGTTCAAGAAAAATATAAATATAAACATTTCANTTTTTTGTATTTTATTAATATAATTTAAACCATTCATCGACANNNAAAAAAAAAAGAAAAAACTAACACATAANGNGTTTACAAATATAAATTATCAATAAAAGTCTCATCGTCAATCCCACCAAAGACGAAGAAGGGTTATCTCCACTCAAACATGTGTCATGCAATTGAAAACGATAAAAATACGTACNGTGGGANTAAAGTGATACGTTTTTCCGGCNTTTTTTGCANCATAACTCCAGTTTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAANANACATCANTTTCATNTTTTTTCTAACCATGATAAAACTACTACAAAACAAGTTAAAAGGGATACGATGTACATCAAACTNATAAGCTTGCGAAAATTATCAATAAAAAGTGATCCAAGAANATAAAANTATGATAATCCACATGTGNTTGCTAACAAATTGAAGATTNGATTACTTGAANTAGAAGCTAAAAGCCAATCCCAATTTTCCAAGGAACAAATCTTCTTTGATTTTNTTGGGTAAATCGTTGAACCAACGACACATAAGTANAGAGAATCTGCATATAAGGTAGAAGAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTACTGACAAAACCGTGACATAAACCAGATATATTGGATGGCAAATTTNGATCNCATCCAAACTNGAAAACTGNCAAAACATACTGGAAAACGAAATAAAATAGAGANNNNATCCGGTAGGAGANATTTTTAAAATTTTCACATTNATTAACTNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.211893
PRIMER_LEFT_0_PENALTY=2.876993
PRIMER_RIGHT_0_PENALTY=0.334900
PRIMER_LEFT_0_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_0_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_0=380,22
PRIMER_RIGHT_0=1374,20
PRIMER_LEFT_0_TM=60.877
PRIMER_RIGHT_0_TM=60.335
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=995
PRIMER_PAIR_1_PENALTY=3.841110
PRIMER_LEFT_1_PENALTY=3.506211
PRIMER_RIGHT_1_PENALTY=0.334900
PRIMER_LEFT_1_SEQUENCE=CCAATGTTTTTGTAGAGCGA
PRIMER_RIGHT_1_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_1=380,20
PRIMER_RIGHT_1=1374,20
PRIMER_LEFT_1_TM=56.494
PRIMER_RIGHT_1_TM=60.335
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=995
PRIMER_PAIR_2_PENALTY=3.895848
PRIMER_LEFT_2_PENALTY=3.560949
PRIMER_RIGHT_2_PENALTY=0.334900
PRIMER_LEFT_2_SEQUENCE=CAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_2_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_2=381,21
PRIMER_RIGHT_2=1374,20
PRIMER_LEFT_2_TM=57.439
PRIMER_RIGHT_2_TM=60.335
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=994
PRIMER_PAIR_3_PENALTY=4.173445
PRIMER_LEFT_3_PENALTY=2.876993
PRIMER_RIGHT_3_PENALTY=1.296452
PRIMER_LEFT_3_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_3_SEQUENCE=CTTTGGTGGGATTGACGATG
PRIMER_LEFT_3=380,22
PRIMER_RIGHT_3=1378,20
PRIMER_LEFT_3_TM=60.877
PRIMER_RIGHT_3_TM=61.296
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=999
PRIMER_PAIR_4_PENALTY=4.336084
PRIMER_LEFT_4_PENALTY=2.876993
PRIMER_RIGHT_4_PENALTY=1.459091
PRIMER_LEFT_4_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_4_SEQUENCE=TGGGATTGACGATGAGACTTT
PRIMER_LEFT_4=380,22
PRIMER_RIGHT_4=1372,21
PRIMER_LEFT_4_TM=60.877
PRIMER_RIGHT_4_TM=59.541
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=993
PRIMER_PAIR_5_PENALTY=4.463664
PRIMER_LEFT_5_PENALTY=3.560949
PRIMER_RIGHT_5_PENALTY=0.902715
PRIMER_LEFT_5_SEQUENCE=CAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_5_SEQUENCE=GTCTTTGGTGGGATTGACGA
PRIMER_LEFT_5=381,21
PRIMER_RIGHT_5=1380,20
PRIMER_LEFT_5_TM=57.439
PRIMER_RIGHT_5_TM=60.903
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=1000
PRIMER_PAIR_6_PENALTY=4.746114
PRIMER_LEFT_6_PENALTY=4.521923
PRIMER_RIGHT_6_PENALTY=0.224191
PRIMER_LEFT_6_SEQUENCE=TCAGTGACTAAGCAGAAGCAA
PRIMER_RIGHT_6_SEQUENCE=CTTCTTCGTCTTTGGTGGGA
PRIMER_LEFT_6=530,21
PRIMER_RIGHT_6=1387,20
PRIMER_LEFT_6_TM=56.478
PRIMER_RIGHT_6_TM=60.224
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=858
PRIMER_PAIR_7_PENALTY=4.753127
PRIMER_LEFT_7_PENALTY=2.876993
PRIMER_RIGHT_7_PENALTY=1.876134
PRIMER_LEFT_7_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_7_SEQUENCE=TTGGTGGGATTGACGATGAG
PRIMER_LEFT_7=380,22
PRIMER_RIGHT_7=1376,20
PRIMER_LEFT_7_TM=60.877
PRIMER_RIGHT_7_TM=61.876
PRIMER_LEFT_7_GC_PERCENT=45.455
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=997
PRIMER_PAIR_8_PENALTY=4.802662
PRIMER_LEFT_8_PENALTY=3.506211
PRIMER_RIGHT_8_PENALTY=1.296452
PRIMER_LEFT_8_SEQUENCE=CCAATGTTTTTGTAGAGCGA
PRIMER_RIGHT_8_SEQUENCE=CTTTGGTGGGATTGACGATG
PRIMER_LEFT_8=380,20
PRIMER_RIGHT_8=1378,20
PRIMER_LEFT_8_TM=56.494
PRIMER_RIGHT_8_TM=61.296
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.9000
PRIMER_RIGHT_8_END_STABILITY=8.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=999
PRIMER_PAIR_9_PENALTY=4.806170
PRIMER_LEFT_9_PENALTY=2.876993
PRIMER_RIGHT_9_PENALTY=1.929176
PRIMER_LEFT_9_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_9_SEQUENCE=TGGGATTGACGATGAGACTT
PRIMER_LEFT_9=380,22
PRIMER_RIGHT_9=1372,20
PRIMER_LEFT_9_TM=60.877
PRIMER_RIGHT_9_TM=58.071
PRIMER_LEFT_9_GC_PERCENT=45.455
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=993
PRIMER_PAIR_10_PENALTY=4.856822
PRIMER_LEFT_10_PENALTY=4.521923
PRIMER_RIGHT_10_PENALTY=0.334900
PRIMER_LEFT_10_SEQUENCE=TCAGTGACTAAGCAGAAGCAA
PRIMER_RIGHT_10_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_10=530,21
PRIMER_RIGHT_10=1374,20
PRIMER_LEFT_10_TM=56.478
PRIMER_RIGHT_10_TM=60.335
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=845
PRIMER_PAIR_11_PENALTY=4.857400
PRIMER_LEFT_11_PENALTY=3.560949
PRIMER_RIGHT_11_PENALTY=1.296452
PRIMER_LEFT_11_SEQUENCE=CAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_11_SEQUENCE=CTTTGGTGGGATTGACGATG
PRIMER_LEFT_11=381,21
PRIMER_RIGHT_11=1378,20
PRIMER_LEFT_11_TM=57.439
PRIMER_RIGHT_11_TM=61.296
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.6000
PRIMER_RIGHT_11_END_STABILITY=8.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=998
PRIMER_PAIR_12_PENALTY=4.870423
PRIMER_LEFT_12_PENALTY=2.876993
PRIMER_RIGHT_12_PENALTY=1.993430
PRIMER_LEFT_12_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_12_SEQUENCE=GTGGGATTGACGATGAGACTT
PRIMER_LEFT_12=380,22
PRIMER_RIGHT_12=1373,21
PRIMER_LEFT_12_TM=60.877
PRIMER_RIGHT_12_TM=59.007
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=994
PRIMER_PAIR_13_PENALTY=4.965301
PRIMER_LEFT_13_PENALTY=3.506211
PRIMER_RIGHT_13_PENALTY=1.459091
PRIMER_LEFT_13_SEQUENCE=CCAATGTTTTTGTAGAGCGA
PRIMER_RIGHT_13_SEQUENCE=TGGGATTGACGATGAGACTTT
PRIMER_LEFT_13=380,20
PRIMER_RIGHT_13=1372,21
PRIMER_LEFT_13_TM=56.494
PRIMER_RIGHT_13_TM=59.541
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=993
PRIMER_PAIR_14_PENALTY=4.995617
PRIMER_LEFT_14_PENALTY=4.521923
PRIMER_RIGHT_14_PENALTY=0.473695
PRIMER_LEFT_14_SEQUENCE=TCAGTGACTAAGCAGAAGCAA
PRIMER_RIGHT_14_SEQUENCE=TCTTCGTCTTTGGTGGGATT
PRIMER_LEFT_14=530,21
PRIMER_RIGHT_14=1385,20
PRIMER_LEFT_14_TM=56.478
PRIMER_RIGHT_14_TM=59.526
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=856
PRIMER_PAIR_15_PENALTY=5.007995
PRIMER_LEFT_15_PENALTY=4.521923
PRIMER_RIGHT_15_PENALTY=0.486072
PRIMER_LEFT_15_SEQUENCE=TCAGTGACTAAGCAGAAGCAA
PRIMER_RIGHT_15_SEQUENCE=CTTCGTCTTTGGTGGGATTG
PRIMER_LEFT_15=530,21
PRIMER_RIGHT_15=1384,20
PRIMER_LEFT_15_TM=56.478
PRIMER_RIGHT_15_TM=60.486
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=855
PRIMER_PAIR_16_PENALTY=5.020039
PRIMER_LEFT_16_PENALTY=3.560949
PRIMER_RIGHT_16_PENALTY=1.459091
PRIMER_LEFT_16_SEQUENCE=CAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_16_SEQUENCE=TGGGATTGACGATGAGACTTT
PRIMER_LEFT_16=381,21
PRIMER_RIGHT_16=1372,21
PRIMER_LEFT_16_TM=57.439
PRIMER_RIGHT_16_TM=59.541
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=992
PRIMER_PAIR_17_PENALTY=5.021015
PRIMER_LEFT_17_PENALTY=4.796824
PRIMER_RIGHT_17_PENALTY=0.224191
PRIMER_LEFT_17_SEQUENCE=GATGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_17_SEQUENCE=CTTCTTCGTCTTTGGTGGGA
PRIMER_LEFT_17=679,24
PRIMER_RIGHT_17=1387,20
PRIMER_LEFT_17_TM=59.203
PRIMER_RIGHT_17_TM=60.224
PRIMER_LEFT_17_GC_PERCENT=41.667
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=709
PRIMER_PAIR_18_PENALTY=5.131724
PRIMER_LEFT_18_PENALTY=4.796824
PRIMER_RIGHT_18_PENALTY=0.334900
PRIMER_LEFT_18_SEQUENCE=GATGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_18_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_18=679,24
PRIMER_RIGHT_18=1374,20
PRIMER_LEFT_18_TM=59.203
PRIMER_RIGHT_18_TM=60.335
PRIMER_LEFT_18_GC_PERCENT=41.667
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=696
PRIMER_PAIR_19_PENALTY=5.136199
PRIMER_LEFT_19_PENALTY=2.876993
PRIMER_RIGHT_19_PENALTY=2.259206
PRIMER_LEFT_19_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_19_SEQUENCE=TTTGGTGGGATTGACGATGA
PRIMER_LEFT_19=380,22
PRIMER_RIGHT_19=1377,20
PRIMER_LEFT_19_TM=60.877
PRIMER_RIGHT_19_TM=62.259
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=998
PRIMER_PAIR_20_PENALTY=5.190914
PRIMER_LEFT_20_PENALTY=4.966722
PRIMER_RIGHT_20_PENALTY=0.224191
PRIMER_LEFT_20_SEQUENCE=GACGATCAGTCGTAAGAAAACA
PRIMER_RIGHT_20_SEQUENCE=CTTCTTCGTCTTTGGTGGGA
PRIMER_LEFT_20=682,22
PRIMER_RIGHT_20=1387,20
PRIMER_LEFT_20_TM=57.033
PRIMER_RIGHT_20_TM=60.224
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=706
PRIMER_PAIR_21_PENALTY=5.190914
PRIMER_LEFT_21_PENALTY=4.966722
PRIMER_RIGHT_21_PENALTY=0.224191
PRIMER_LEFT_21_SEQUENCE=TGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_21_SEQUENCE=CTTCTTCGTCTTTGGTGGGA
PRIMER_LEFT_21=681,22
PRIMER_RIGHT_21=1387,20
PRIMER_LEFT_21_TM=57.033
PRIMER_RIGHT_21_TM=60.224
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=707
PRIMER_PAIR_22_PENALTY=5.246622
PRIMER_LEFT_22_PENALTY=2.876993
PRIMER_RIGHT_22_PENALTY=2.369628
PRIMER_LEFT_22_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_22_SEQUENCE=GTGGGATTGACGATGAGACTTT
PRIMER_LEFT_22=380,22
PRIMER_RIGHT_22=1373,22
PRIMER_LEFT_22_TM=60.877
PRIMER_RIGHT_22_TM=60.370
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=994
PRIMER_PAIR_23_PENALTY=5.254742
PRIMER_LEFT_23_PENALTY=5.030551
PRIMER_RIGHT_23_PENALTY=0.224191
PRIMER_LEFT_23_SEQUENCE=GATGACGATCAGTCGTAAGAAA
PRIMER_RIGHT_23_SEQUENCE=CTTCTTCGTCTTTGGTGGGA
PRIMER_LEFT_23=679,22
PRIMER_RIGHT_23=1387,20
PRIMER_LEFT_23_TM=56.969
PRIMER_RIGHT_23_TM=60.224
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=709
PRIMER_PAIR_24_PENALTY=5.270519
PRIMER_LEFT_24_PENALTY=4.796824
PRIMER_RIGHT_24_PENALTY=0.473695
PRIMER_LEFT_24_SEQUENCE=GATGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_24_SEQUENCE=TCTTCGTCTTTGGTGGGATT
PRIMER_LEFT_24=679,24
PRIMER_RIGHT_24=1385,20
PRIMER_LEFT_24_TM=59.203
PRIMER_RIGHT_24_TM=59.526
PRIMER_LEFT_24_GC_PERCENT=41.667
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=707
PRIMER_PAIR_25_PENALTY=5.282896
PRIMER_LEFT_25_PENALTY=4.796824
PRIMER_RIGHT_25_PENALTY=0.486072
PRIMER_LEFT_25_SEQUENCE=GATGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_25_SEQUENCE=CTTCGTCTTTGGTGGGATTG
PRIMER_LEFT_25=679,24
PRIMER_RIGHT_25=1384,20
PRIMER_LEFT_25_TM=59.203
PRIMER_RIGHT_25_TM=60.486
PRIMER_LEFT_25_GC_PERCENT=41.667
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=706
PRIMER_PAIR_26_PENALTY=5.301622
PRIMER_LEFT_26_PENALTY=4.966722
PRIMER_RIGHT_26_PENALTY=0.334900
PRIMER_LEFT_26_SEQUENCE=GACGATCAGTCGTAAGAAAACA
PRIMER_RIGHT_26_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_26=682,22
PRIMER_RIGHT_26=1374,20
PRIMER_LEFT_26_TM=57.033
PRIMER_RIGHT_26_TM=60.335
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.1000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=693
PRIMER_PAIR_27_PENALTY=5.301622
PRIMER_LEFT_27_PENALTY=4.966722
PRIMER_RIGHT_27_PENALTY=0.334900
PRIMER_LEFT_27_SEQUENCE=TGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_27_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_27=681,22
PRIMER_RIGHT_27=1374,20
PRIMER_LEFT_27_TM=57.033
PRIMER_RIGHT_27_TM=60.335
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.1000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=694
PRIMER_PAIR_28_PENALTY=5.338859
PRIMER_LEFT_28_PENALTY=4.521923
PRIMER_RIGHT_28_PENALTY=0.816936
PRIMER_LEFT_28_SEQUENCE=TCAGTGACTAAGCAGAAGCAA
PRIMER_RIGHT_28_SEQUENCE=ACCCTTCTTCGTCTTTGGTG
PRIMER_LEFT_28=530,21
PRIMER_RIGHT_28=1390,20
PRIMER_LEFT_28_TM=56.478
PRIMER_RIGHT_28_TM=59.183
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=861
PRIMER_PAIR_29_PENALTY=5.363178
PRIMER_LEFT_29_PENALTY=2.876993
PRIMER_RIGHT_29_PENALTY=2.486184
PRIMER_LEFT_29_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_29_SEQUENCE=TGGTGGGATTGACGATGAGA
PRIMER_LEFT_29=380,22
PRIMER_RIGHT_29=1375,20
PRIMER_LEFT_29_TM=60.877
PRIMER_RIGHT_29_TM=62.486
PRIMER_LEFT_29_GC_PERCENT=45.455
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.6000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=996
PRIMER_PAIR_30_PENALTY=5.365451
PRIMER_LEFT_30_PENALTY=5.030551
PRIMER_RIGHT_30_PENALTY=0.334900
PRIMER_LEFT_30_SEQUENCE=GATGACGATCAGTCGTAAGAAA
PRIMER_RIGHT_30_SEQUENCE=GGTGGGATTGACGATGAGAC
PRIMER_LEFT_30=679,22
PRIMER_RIGHT_30=1374,20
PRIMER_LEFT_30_TM=56.969
PRIMER_RIGHT_30_TM=60.335
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.1000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=696
PRIMER_PAIR_31_PENALTY=5.382345
PRIMER_LEFT_31_PENALTY=3.506211
PRIMER_RIGHT_31_PENALTY=1.876134
PRIMER_LEFT_31_SEQUENCE=CCAATGTTTTTGTAGAGCGA
PRIMER_RIGHT_31_SEQUENCE=TTGGTGGGATTGACGATGAG
PRIMER_LEFT_31=380,20
PRIMER_RIGHT_31=1376,20
PRIMER_LEFT_31_TM=56.494
PRIMER_RIGHT_31_TM=61.876
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.9000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=997
PRIMER_PAIR_32_PENALTY=5.424638
PRIMER_LEFT_32_PENALTY=4.521923
PRIMER_RIGHT_32_PENALTY=0.902715
PRIMER_LEFT_32_SEQUENCE=TCAGTGACTAAGCAGAAGCAA
PRIMER_RIGHT_32_SEQUENCE=GTCTTTGGTGGGATTGACGA
PRIMER_LEFT_32=530,21
PRIMER_RIGHT_32=1380,20
PRIMER_LEFT_32_TM=56.478
PRIMER_RIGHT_32_TM=60.903
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=851
PRIMER_PAIR_33_PENALTY=5.424638
PRIMER_LEFT_33_PENALTY=4.521923
PRIMER_RIGHT_33_PENALTY=0.902715
PRIMER_LEFT_33_SEQUENCE=TCAGTGACTAAGCAGAAGCAA
PRIMER_RIGHT_33_SEQUENCE=TCGTCTTTGGTGGGATTGAC
PRIMER_LEFT_33=530,21
PRIMER_RIGHT_33=1382,20
PRIMER_LEFT_33_TM=56.478
PRIMER_RIGHT_33_TM=60.903
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=853
PRIMER_PAIR_34_PENALTY=5.435387
PRIMER_LEFT_34_PENALTY=3.506211
PRIMER_RIGHT_34_PENALTY=1.929176
PRIMER_LEFT_34_SEQUENCE=CCAATGTTTTTGTAGAGCGA
PRIMER_RIGHT_34_SEQUENCE=TGGGATTGACGATGAGACTT
PRIMER_LEFT_34=380,20
PRIMER_RIGHT_34=1372,20
PRIMER_LEFT_34_TM=56.494
PRIMER_RIGHT_34_TM=58.071
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.9000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=993
PRIMER_PAIR_35_PENALTY=5.437083
PRIMER_LEFT_35_PENALTY=3.560949
PRIMER_RIGHT_35_PENALTY=1.876134
PRIMER_LEFT_35_SEQUENCE=CAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_35_SEQUENCE=TTGGTGGGATTGACGATGAG
PRIMER_LEFT_35=381,21
PRIMER_RIGHT_35=1376,20
PRIMER_LEFT_35_TM=57.439
PRIMER_RIGHT_35_TM=61.876
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.6000
PRIMER_RIGHT_35_END_STABILITY=6.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=996
PRIMER_PAIR_36_PENALTY=5.440417
PRIMER_LEFT_36_PENALTY=4.966722
PRIMER_RIGHT_36_PENALTY=0.473695
PRIMER_LEFT_36_SEQUENCE=GACGATCAGTCGTAAGAAAACA
PRIMER_RIGHT_36_SEQUENCE=TCTTCGTCTTTGGTGGGATT
PRIMER_LEFT_36=682,22
PRIMER_RIGHT_36=1385,20
PRIMER_LEFT_36_TM=57.033
PRIMER_RIGHT_36_TM=59.526
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.1000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=704
PRIMER_PAIR_37_PENALTY=5.440417
PRIMER_LEFT_37_PENALTY=4.966722
PRIMER_RIGHT_37_PENALTY=0.473695
PRIMER_LEFT_37_SEQUENCE=TGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_37_SEQUENCE=TCTTCGTCTTTGGTGGGATT
PRIMER_LEFT_37=681,22
PRIMER_RIGHT_37=1385,20
PRIMER_LEFT_37_TM=57.033
PRIMER_RIGHT_37_TM=59.526
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=8.1000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=705
PRIMER_PAIR_38_PENALTY=5.452795
PRIMER_LEFT_38_PENALTY=4.966722
PRIMER_RIGHT_38_PENALTY=0.486072
PRIMER_LEFT_38_SEQUENCE=GACGATCAGTCGTAAGAAAACA
PRIMER_RIGHT_38_SEQUENCE=CTTCGTCTTTGGTGGGATTG
PRIMER_LEFT_38=682,22
PRIMER_RIGHT_38=1384,20
PRIMER_LEFT_38_TM=57.033
PRIMER_RIGHT_38_TM=60.486
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=703
PRIMER_PAIR_39_PENALTY=5.452795
PRIMER_LEFT_39_PENALTY=4.966722
PRIMER_RIGHT_39_PENALTY=0.486072
PRIMER_LEFT_39_SEQUENCE=TGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_39_SEQUENCE=CTTCGTCTTTGGTGGGATTG
PRIMER_LEFT_39=681,22
PRIMER_RIGHT_39=1384,20
PRIMER_LEFT_39_TM=57.033
PRIMER_RIGHT_39_TM=60.486
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=704
PRIMER_PAIR_40_PENALTY=5.490125
PRIMER_LEFT_40_PENALTY=3.560949
PRIMER_RIGHT_40_PENALTY=1.929176
PRIMER_LEFT_40_SEQUENCE=CAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_40_SEQUENCE=TGGGATTGACGATGAGACTT
PRIMER_LEFT_40=381,21
PRIMER_RIGHT_40=1372,20
PRIMER_LEFT_40_TM=57.439
PRIMER_RIGHT_40_TM=58.071
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=992
PRIMER_PAIR_41_PENALTY=5.499640
PRIMER_LEFT_41_PENALTY=3.506211
PRIMER_RIGHT_41_PENALTY=1.993430
PRIMER_LEFT_41_SEQUENCE=CCAATGTTTTTGTAGAGCGA
PRIMER_RIGHT_41_SEQUENCE=GTGGGATTGACGATGAGACTT
PRIMER_LEFT_41=380,20
PRIMER_RIGHT_41=1373,21
PRIMER_LEFT_41_TM=56.494
PRIMER_RIGHT_41_TM=59.007
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=994
PRIMER_PAIR_42_PENALTY=5.504246
PRIMER_LEFT_42_PENALTY=5.030551
PRIMER_RIGHT_42_PENALTY=0.473695
PRIMER_LEFT_42_SEQUENCE=GATGACGATCAGTCGTAAGAAA
PRIMER_RIGHT_42_SEQUENCE=TCTTCGTCTTTGGTGGGATT
PRIMER_LEFT_42=679,22
PRIMER_RIGHT_42=1385,20
PRIMER_LEFT_42_TM=56.969
PRIMER_RIGHT_42_TM=59.526
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.1000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=707
PRIMER_PAIR_43_PENALTY=5.516623
PRIMER_LEFT_43_PENALTY=5.030551
PRIMER_RIGHT_43_PENALTY=0.486072
PRIMER_LEFT_43_SEQUENCE=GATGACGATCAGTCGTAAGAAA
PRIMER_RIGHT_43_SEQUENCE=CTTCGTCTTTGGTGGGATTG
PRIMER_LEFT_43=679,22
PRIMER_RIGHT_43=1384,20
PRIMER_LEFT_43_TM=56.969
PRIMER_RIGHT_43_TM=60.486
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=706
PRIMER_PAIR_44_PENALTY=5.554378
PRIMER_LEFT_44_PENALTY=3.560949
PRIMER_RIGHT_44_PENALTY=1.993430
PRIMER_LEFT_44_SEQUENCE=CAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_44_SEQUENCE=GTGGGATTGACGATGAGACTT
PRIMER_LEFT_44=381,21
PRIMER_RIGHT_44=1373,21
PRIMER_LEFT_44_TM=57.439
PRIMER_RIGHT_44_TM=59.007
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=993
PRIMER_PAIR_45_PENALTY=5.613760
PRIMER_LEFT_45_PENALTY=4.796824
PRIMER_RIGHT_45_PENALTY=0.816936
PRIMER_LEFT_45_SEQUENCE=GATGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_45_SEQUENCE=ACCCTTCTTCGTCTTTGGTG
PRIMER_LEFT_45=679,24
PRIMER_RIGHT_45=1390,20
PRIMER_LEFT_45_TM=59.203
PRIMER_RIGHT_45_TM=59.183
PRIMER_LEFT_45_GC_PERCENT=41.667
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=712
PRIMER_PAIR_46_PENALTY=5.699539
PRIMER_LEFT_46_PENALTY=4.796824
PRIMER_RIGHT_46_PENALTY=0.902715
PRIMER_LEFT_46_SEQUENCE=GATGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_46_SEQUENCE=GTCTTTGGTGGGATTGACGA
PRIMER_LEFT_46=679,24
PRIMER_RIGHT_46=1380,20
PRIMER_LEFT_46_TM=59.203
PRIMER_RIGHT_46_TM=60.903
PRIMER_LEFT_46_GC_PERCENT=41.667
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=702
PRIMER_PAIR_47_PENALTY=5.699539
PRIMER_LEFT_47_PENALTY=4.796824
PRIMER_RIGHT_47_PENALTY=0.902715
PRIMER_LEFT_47_SEQUENCE=GATGACGATCAGTCGTAAGAAAAC
PRIMER_RIGHT_47_SEQUENCE=TCGTCTTTGGTGGGATTGAC
PRIMER_LEFT_47=679,24
PRIMER_RIGHT_47=1382,20
PRIMER_LEFT_47_TM=59.203
PRIMER_RIGHT_47_TM=60.903
PRIMER_LEFT_47_GC_PERCENT=41.667
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=704
PRIMER_PAIR_48_PENALTY=5.732615
PRIMER_LEFT_48_PENALTY=2.876993
PRIMER_RIGHT_48_PENALTY=2.855622
PRIMER_LEFT_48_SEQUENCE=CCAATGTTTTTGTAGAGCGACC
PRIMER_RIGHT_48_SEQUENCE=TGGGATTGACGATGAGACTTTT
PRIMER_LEFT_48=380,22
PRIMER_RIGHT_48=1372,22
PRIMER_LEFT_48_TM=60.877
PRIMER_RIGHT_48_TM=60.856
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=993
PRIMER_PAIR_49_PENALTY=5.765416
PRIMER_LEFT_49_PENALTY=3.506211
PRIMER_RIGHT_49_PENALTY=2.259206
PRIMER_LEFT_49_SEQUENCE=CCAATGTTTTTGTAGAGCGA
PRIMER_RIGHT_49_SEQUENCE=TTTGGTGGGATTGACGATGA
PRIMER_LEFT_49=380,20
PRIMER_RIGHT_49=1377,20
PRIMER_LEFT_49_TM=56.494
PRIMER_RIGHT_49_TM=62.259
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=998
=
SEQUENCE_ID=MAGIC.433_4_4449362_4449380_tsu-0_oy-0_44476_36132_TRUE
SEQUENCE_TARGET=950,118
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTCGATATTATTCACNAAATCTTGNGTTTTACTTNGAGNTAACTCCAAATCTNATGAAAACCCTAGNACTAAGCTAAANNCTACTNTATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNACACATTTTCTTCCCACATNNNTAAATTTGAATTTATTANAGNTANNCACCANAAGCAAACNATGCCAAGANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTACATTTGATGAAGTAGCTGATCNTTTTACTTNGANCAGAAAANNNNAAAAGTTCCAACTAAGGNAAAGATCTNCCAAAATTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTNTCNNAGNTCCAAAAAATNACAAAGAGNTGAGGACGTGNGATGGTGTTGTGTATGAGACNTACACTGAAGTNTGTGCNGNNCGAGNATTTATGTAANAAGAGNGNNNNNANNCCNTNNNNNNNAANNTNTNCNATTCAATTTNCTTTAAGTTCTATNTNTTTATTTTCAATTNCTNCTTTGTTATTCANACNNNNNNNTAATTCATGTTTNANTTATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_1799703_1799912_tsu-0_ct-1_1799565_1531_TRUE
SEQUENCE_TARGET=950,309
SEQUENCE_TEMPLATE=GCCATTTTCAATGGCATTGGAGGCCATGTGAACTCCAAAGGCTGAACTTGAGTAGCTCTTAGATCGGTGTTAAATATCCTCGACGGCGGAAGGTTCCAGAAACCAGAAACCTCTAGTTTCCTATTGTTTGGTACTGTCGAATTTAACGAATAAGGCTCCCAAAACCTATCAAACTGATCATCTGGAAACCTAAACAAGAAGAATGTTGATAATGGTGAATACATNGCCTTGGTTTCGAAAGATTTAGCATCGAAGGAACAATTTTTCTGAGAAAGTATCAAACTTTNNATCAAAGTAGTGAACNTTTTTACCTGATGATTGGTCCACTGTAACCAAAAGCATGCCTAGCAACAAGACTCATGCCAACAGTGGTAAAGTCAGTGGAATTGTAAAGAGTACCATCAAGCCTAACGAGCTCCAAAGCTGAGATAAATGGATCAGAAGTTGTATAAGAATTAGAAGCAACGCAGACGCTTATAGATTTCCCCTGCGCCAGAAAAACCCCTTCGTAATAAGAAGCGAGACCATCTGCGTAATCGGCGGTNGTATTGACGATCCCCCATAACGTGCCGTCGACAATCTGATCAAATACCGGCGGNGGAGTACCTTTACCGTTAACTCCTCCGTAATAGTATGTCGTCCTGATCATATACTTCCAGCCACGAGAAACGCTCATCACGTAGCAAAACTTGTGATGAGTTCCGTCGAGGGAAAGAGGGAAAGATCTNACAGTGAATAAGATTTCGTCGAGGACTTGATCGGANACGTTTTTNGGTGTGCCGCTGGAGACGAAANTCTCGTCGGGTTGCCATTGTCGGCCGTCGATTACGGAGGAGGAAGAGGCGCCACAATCGATCAGAATNGCTAAATTNTAAAATTAGAAATGTNACAAAATTGGAAANTGGAAGGGATANGAATTTAGGCNATGGNNNNAAGAGATTGAGGTGATACCTGAAGGAGNAGAGGGCTGAGAGAGGGAAGAGGAGANGAGGGAGAAGACNGNGAGGAGGAGGAGGAGGAGNGGGGACATGGCGGCGGAGACGNNGGTGGNAGAGAAGAAGTTAGCATNAAAGAGAGGTTTAGGTTGGAAGAAGGAGGGGGGTGGGTTGACCCTCCAGCTCCAGAGAATTTGAAGAATGAGAGAATTAGAGGAACAGGTCAGAGAGGTAGCNNNNGNNGANNNNNTTNNNNNNNNNNNNTTNATTNNTTNTNNGANNNNNANANNTTNTTNTNTTNNTCNNTTNNTTNTNTTNNNGTCNANNNNGNNANNNNNNAATTATNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNTNNNNNNNAANNANNNNTNNNTNTNNAATNNNNNCCNNNNNNNNNNNNNNNNNNANNNTNNNNTTNANNNNNNNNNNNNNNNNNNNTNANNNNNNNNTTTANNTAATNNTNNTTNNNNANNNNNNTNNNNNATNNNGNTTNNNNNNNNNNNNNTANNNNTTNNNANNNNNNNTTNNNAANNNNNNNNNNATNNNNTNNAANNNNNCNATNNNCNNNNNNNNTTTTCANNNNTNANNNNTNNNNANNNTNNNNCNNNTCNNTNNNNTNATANNTNTNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACACCTATGGATCGAGATCAAACATCANACCCTACATAACCAANCCTGTTCTAATTAACATTANTAACTTTAATTANNATATCTTAAACTTCATANNATTTGGAGNCTCNTNTTAAGTAGTGTTTACGTTTNCTCACTTTTCTTTGCNTCTTCAATGAAAAATTGCATTAAAGCTTTCGGATGCNAAGCGTGCTCGTGGTGAATCACAAGANTTCATTTCATGTGCTCGCATTAATAAAATATAACATTGGTATCTNTTTGCATTTTNTTCAAAGCTCATACACGTTTACTTTGTGGCCNACCACAACGGTCCAAAAAGTCTCTAAAATATTACTTATTTTGTAAACTATCGTNAAAATACGGAAAACATCCANCTCAATGAATATTTAGGATGAGTAGAGCTGTACGTAATAAGAGGATGTTGATGCTAAGATAACGTTTATTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.556888
PRIMER_LEFT_0_PENALTY=0.182658
PRIMER_RIGHT_0_PENALTY=3.374230
PRIMER_LEFT_0_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_0_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_0=703,20
PRIMER_RIGHT_0=1696,21
PRIMER_LEFT_0_TM=60.183
PRIMER_RIGHT_0_TM=57.626
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=994
PRIMER_PAIR_1_PENALTY=3.607066
PRIMER_LEFT_1_PENALTY=0.232835
PRIMER_RIGHT_1_PENALTY=3.374230
PRIMER_LEFT_1_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_1_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_1=821,20
PRIMER_RIGHT_1=1696,21
PRIMER_LEFT_1_TM=59.767
PRIMER_RIGHT_1_TM=57.626
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=876
PRIMER_PAIR_2_PENALTY=3.653114
PRIMER_LEFT_2_PENALTY=0.182658
PRIMER_RIGHT_2_PENALTY=3.470456
PRIMER_LEFT_2_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_2_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_2=703,20
PRIMER_RIGHT_2=1696,22
PRIMER_LEFT_2_TM=60.183
PRIMER_RIGHT_2_TM=58.530
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=994
PRIMER_PAIR_3_PENALTY=3.653114
PRIMER_LEFT_3_PENALTY=0.182658
PRIMER_RIGHT_3_PENALTY=3.470456
PRIMER_LEFT_3_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_3_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_3=703,20
PRIMER_RIGHT_3=1697,22
PRIMER_LEFT_3_TM=60.183
PRIMER_RIGHT_3_TM=58.530
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=995
PRIMER_PAIR_4_PENALTY=3.703292
PRIMER_LEFT_4_PENALTY=0.232835
PRIMER_RIGHT_4_PENALTY=3.470456
PRIMER_LEFT_4_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_4_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_4=821,20
PRIMER_RIGHT_4=1696,22
PRIMER_LEFT_4_TM=59.767
PRIMER_RIGHT_4_TM=58.530
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=876
PRIMER_PAIR_5_PENALTY=3.703292
PRIMER_LEFT_5_PENALTY=0.232835
PRIMER_RIGHT_5_PENALTY=3.470456
PRIMER_LEFT_5_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_5_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_5=821,20
PRIMER_RIGHT_5=1697,22
PRIMER_LEFT_5_TM=59.767
PRIMER_RIGHT_5_TM=58.530
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=877
PRIMER_PAIR_6_PENALTY=3.816697
PRIMER_LEFT_6_PENALTY=0.182658
PRIMER_RIGHT_6_PENALTY=3.634039
PRIMER_LEFT_6_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_6_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_6=703,20
PRIMER_RIGHT_6=1697,23
PRIMER_LEFT_6_TM=60.183
PRIMER_RIGHT_6_TM=59.366
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=995
PRIMER_PAIR_7_PENALTY=3.866874
PRIMER_LEFT_7_PENALTY=0.232835
PRIMER_RIGHT_7_PENALTY=3.634039
PRIMER_LEFT_7_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_7_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_7=821,20
PRIMER_RIGHT_7=1697,23
PRIMER_LEFT_7_TM=59.767
PRIMER_RIGHT_7_TM=59.366
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=877
PRIMER_PAIR_8_PENALTY=4.122196
PRIMER_LEFT_8_PENALTY=0.182658
PRIMER_RIGHT_8_PENALTY=3.939538
PRIMER_LEFT_8_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_8_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_8=703,20
PRIMER_RIGHT_8=1695,21
PRIMER_LEFT_8_TM=60.183
PRIMER_RIGHT_8_TM=57.060
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=993
PRIMER_PAIR_9_PENALTY=4.142091
PRIMER_LEFT_9_PENALTY=0.182658
PRIMER_RIGHT_9_PENALTY=3.959434
PRIMER_LEFT_9_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_9_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_9=703,20
PRIMER_RIGHT_9=1695,20
PRIMER_LEFT_9_TM=60.183
PRIMER_RIGHT_9_TM=56.041
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=993
PRIMER_PAIR_10_PENALTY=4.172373
PRIMER_LEFT_10_PENALTY=0.232835
PRIMER_RIGHT_10_PENALTY=3.939538
PRIMER_LEFT_10_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_10_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_10=821,20
PRIMER_RIGHT_10=1695,21
PRIMER_LEFT_10_TM=59.767
PRIMER_RIGHT_10_TM=57.060
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=875
PRIMER_PAIR_11_PENALTY=4.192269
PRIMER_LEFT_11_PENALTY=0.232835
PRIMER_RIGHT_11_PENALTY=3.959434
PRIMER_LEFT_11_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_11_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_11=821,20
PRIMER_RIGHT_11=1695,20
PRIMER_LEFT_11_TM=59.767
PRIMER_RIGHT_11_TM=56.041
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=875
PRIMER_PAIR_12_PENALTY=4.414623
PRIMER_LEFT_12_PENALTY=0.182658
PRIMER_RIGHT_12_PENALTY=4.231965
PRIMER_LEFT_12_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_12_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_12=703,20
PRIMER_RIGHT_12=1698,23
PRIMER_LEFT_12_TM=60.183
PRIMER_RIGHT_12_TM=61.232
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=996
PRIMER_PAIR_13_PENALTY=4.464800
PRIMER_LEFT_13_PENALTY=0.232835
PRIMER_RIGHT_13_PENALTY=4.231965
PRIMER_LEFT_13_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_13_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_13=821,20
PRIMER_RIGHT_13=1698,23
PRIMER_LEFT_13_TM=59.767
PRIMER_RIGHT_13_TM=61.232
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=878
PRIMER_PAIR_14_PENALTY=4.490469
PRIMER_LEFT_14_PENALTY=1.116239
PRIMER_RIGHT_14_PENALTY=3.374230
PRIMER_LEFT_14_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_14_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_14=822,20
PRIMER_RIGHT_14=1696,21
PRIMER_LEFT_14_TM=58.884
PRIMER_RIGHT_14_TM=57.626
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=875
PRIMER_PAIR_15_PENALTY=4.573597
PRIMER_LEFT_15_PENALTY=1.199367
PRIMER_RIGHT_15_PENALTY=3.374230
PRIMER_LEFT_15_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_15_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_15=704,20
PRIMER_RIGHT_15=1696,21
PRIMER_LEFT_15_TM=61.199
PRIMER_RIGHT_15_TM=57.626
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=993
PRIMER_PAIR_16_PENALTY=4.586695
PRIMER_LEFT_16_PENALTY=1.116239
PRIMER_RIGHT_16_PENALTY=3.470456
PRIMER_LEFT_16_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_16_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_16=822,20
PRIMER_RIGHT_16=1696,22
PRIMER_LEFT_16_TM=58.884
PRIMER_RIGHT_16_TM=58.530
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=875
PRIMER_PAIR_17_PENALTY=4.586695
PRIMER_LEFT_17_PENALTY=1.116239
PRIMER_RIGHT_17_PENALTY=3.470456
PRIMER_LEFT_17_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_17_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_17=822,20
PRIMER_RIGHT_17=1697,22
PRIMER_LEFT_17_TM=58.884
PRIMER_RIGHT_17_TM=58.530
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=876
PRIMER_PAIR_18_PENALTY=4.633452
PRIMER_LEFT_18_PENALTY=1.259222
PRIMER_RIGHT_18_PENALTY=3.374230
PRIMER_LEFT_18_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_18_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_18=820,20
PRIMER_RIGHT_18=1696,21
PRIMER_LEFT_18_TM=58.741
PRIMER_RIGHT_18_TM=57.626
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=877
PRIMER_PAIR_19_PENALTY=4.669823
PRIMER_LEFT_19_PENALTY=1.199367
PRIMER_RIGHT_19_PENALTY=3.470456
PRIMER_LEFT_19_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_19_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_19=704,20
PRIMER_RIGHT_19=1696,22
PRIMER_LEFT_19_TM=61.199
PRIMER_RIGHT_19_TM=58.530
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=993
PRIMER_PAIR_20_PENALTY=4.669823
PRIMER_LEFT_20_PENALTY=1.199367
PRIMER_RIGHT_20_PENALTY=3.470456
PRIMER_LEFT_20_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_20_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_20=704,20
PRIMER_RIGHT_20=1697,22
PRIMER_LEFT_20_TM=61.199
PRIMER_RIGHT_20_TM=58.530
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=994
PRIMER_PAIR_21_PENALTY=4.729679
PRIMER_LEFT_21_PENALTY=1.259222
PRIMER_RIGHT_21_PENALTY=3.470456
PRIMER_LEFT_21_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_21_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_21=820,20
PRIMER_RIGHT_21=1696,22
PRIMER_LEFT_21_TM=58.741
PRIMER_RIGHT_21_TM=58.530
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=877
PRIMER_PAIR_22_PENALTY=4.729679
PRIMER_LEFT_22_PENALTY=1.259222
PRIMER_RIGHT_22_PENALTY=3.470456
PRIMER_LEFT_22_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_22_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_22=820,20
PRIMER_RIGHT_22=1697,22
PRIMER_LEFT_22_TM=58.741
PRIMER_RIGHT_22_TM=58.530
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=878
PRIMER_PAIR_23_PENALTY=4.750277
PRIMER_LEFT_23_PENALTY=1.116239
PRIMER_RIGHT_23_PENALTY=3.634039
PRIMER_LEFT_23_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_23_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_23=822,20
PRIMER_RIGHT_23=1697,23
PRIMER_LEFT_23_TM=58.884
PRIMER_RIGHT_23_TM=59.366
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=876
PRIMER_PAIR_24_PENALTY=4.768028
PRIMER_LEFT_24_PENALTY=0.182658
PRIMER_RIGHT_24_PENALTY=4.585370
PRIMER_LEFT_24_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_24_SEQUENCE=TGATCTCGATCCATAGGTGT
PRIMER_LEFT_24=703,20
PRIMER_RIGHT_24=1694,20
PRIMER_LEFT_24_TM=60.183
PRIMER_RIGHT_24_TM=55.415
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=992
PRIMER_PAIR_25_PENALTY=4.818205
PRIMER_LEFT_25_PENALTY=0.232835
PRIMER_RIGHT_25_PENALTY=4.585370
PRIMER_LEFT_25_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_25_SEQUENCE=TGATCTCGATCCATAGGTGT
PRIMER_LEFT_25=821,20
PRIMER_RIGHT_25=1694,20
PRIMER_LEFT_25_TM=59.767
PRIMER_RIGHT_25_TM=55.415
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=874
PRIMER_PAIR_26_PENALTY=4.833405
PRIMER_LEFT_26_PENALTY=1.199367
PRIMER_RIGHT_26_PENALTY=3.634039
PRIMER_LEFT_26_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_26_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_26=704,20
PRIMER_RIGHT_26=1697,23
PRIMER_LEFT_26_TM=61.199
PRIMER_RIGHT_26_TM=59.366
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=994
PRIMER_PAIR_27_PENALTY=4.893261
PRIMER_LEFT_27_PENALTY=1.259222
PRIMER_RIGHT_27_PENALTY=3.634039
PRIMER_LEFT_27_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_27_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_27=820,20
PRIMER_RIGHT_27=1697,23
PRIMER_LEFT_27_TM=58.741
PRIMER_RIGHT_27_TM=59.366
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=878
PRIMER_PAIR_28_PENALTY=4.958263
PRIMER_LEFT_28_PENALTY=1.584033
PRIMER_RIGHT_28_PENALTY=3.374230
PRIMER_LEFT_28_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_28_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_28=820,21
PRIMER_RIGHT_28=1696,21
PRIMER_LEFT_28_TM=60.584
PRIMER_RIGHT_28_TM=57.626
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=877
PRIMER_PAIR_29_PENALTY=5.054489
PRIMER_LEFT_29_PENALTY=1.584033
PRIMER_RIGHT_29_PENALTY=3.470456
PRIMER_LEFT_29_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_29_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_29=820,21
PRIMER_RIGHT_29=1696,22
PRIMER_LEFT_29_TM=60.584
PRIMER_RIGHT_29_TM=58.530
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=877
PRIMER_PAIR_30_PENALTY=5.054489
PRIMER_LEFT_30_PENALTY=1.584033
PRIMER_RIGHT_30_PENALTY=3.470456
PRIMER_LEFT_30_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_30_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_30=820,21
PRIMER_RIGHT_30=1697,22
PRIMER_LEFT_30_TM=60.584
PRIMER_RIGHT_30_TM=58.530
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=878
PRIMER_PAIR_31_PENALTY=5.055777
PRIMER_LEFT_31_PENALTY=1.116239
PRIMER_RIGHT_31_PENALTY=3.939538
PRIMER_LEFT_31_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_31_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_31=822,20
PRIMER_RIGHT_31=1695,21
PRIMER_LEFT_31_TM=58.884
PRIMER_RIGHT_31_TM=57.060
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=874
PRIMER_PAIR_32_PENALTY=5.075672
PRIMER_LEFT_32_PENALTY=1.116239
PRIMER_RIGHT_32_PENALTY=3.959434
PRIMER_LEFT_32_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_32_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_32=822,20
PRIMER_RIGHT_32=1695,20
PRIMER_LEFT_32_TM=58.884
PRIMER_RIGHT_32_TM=56.041
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=874
PRIMER_PAIR_33_PENALTY=5.082730
PRIMER_LEFT_33_PENALTY=1.708500
PRIMER_RIGHT_33_PENALTY=3.374230
PRIMER_LEFT_33_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_33_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_33=821,21
PRIMER_RIGHT_33=1696,21
PRIMER_LEFT_33_TM=60.708
PRIMER_RIGHT_33_TM=57.626
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=876
PRIMER_PAIR_34_PENALTY=5.138905
PRIMER_LEFT_34_PENALTY=1.199367
PRIMER_RIGHT_34_PENALTY=3.939538
PRIMER_LEFT_34_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_34_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_34=704,20
PRIMER_RIGHT_34=1695,21
PRIMER_LEFT_34_TM=61.199
PRIMER_RIGHT_34_TM=57.060
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=992
PRIMER_PAIR_35_PENALTY=5.158800
PRIMER_LEFT_35_PENALTY=1.199367
PRIMER_RIGHT_35_PENALTY=3.959434
PRIMER_LEFT_35_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_35_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_35=704,20
PRIMER_RIGHT_35=1695,20
PRIMER_LEFT_35_TM=61.199
PRIMER_RIGHT_35_TM=56.041
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=5.178956
PRIMER_LEFT_36_PENALTY=1.708500
PRIMER_RIGHT_36_PENALTY=3.470456
PRIMER_LEFT_36_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_36_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_36=821,21
PRIMER_RIGHT_36=1696,22
PRIMER_LEFT_36_TM=60.708
PRIMER_RIGHT_36_TM=58.530
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=876
PRIMER_PAIR_37_PENALTY=5.178956
PRIMER_LEFT_37_PENALTY=1.708500
PRIMER_RIGHT_37_PENALTY=3.470456
PRIMER_LEFT_37_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_37_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_37=821,21
PRIMER_RIGHT_37=1697,22
PRIMER_LEFT_37_TM=60.708
PRIMER_RIGHT_37_TM=58.530
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=877
PRIMER_PAIR_38_PENALTY=5.198760
PRIMER_LEFT_38_PENALTY=1.259222
PRIMER_RIGHT_38_PENALTY=3.939538
PRIMER_LEFT_38_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_38_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_38=820,20
PRIMER_RIGHT_38=1695,21
PRIMER_LEFT_38_TM=58.741
PRIMER_RIGHT_38_TM=57.060
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=876
PRIMER_PAIR_39_PENALTY=5.218072
PRIMER_LEFT_39_PENALTY=1.584033
PRIMER_RIGHT_39_PENALTY=3.634039
PRIMER_LEFT_39_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_39_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_39=820,21
PRIMER_RIGHT_39=1697,23
PRIMER_LEFT_39_TM=60.584
PRIMER_RIGHT_39_TM=59.366
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=43.478
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=878
PRIMER_PAIR_40_PENALTY=5.218656
PRIMER_LEFT_40_PENALTY=1.259222
PRIMER_RIGHT_40_PENALTY=3.959434
PRIMER_LEFT_40_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_40_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_40=820,20
PRIMER_RIGHT_40=1695,20
PRIMER_LEFT_40_TM=58.741
PRIMER_RIGHT_40_TM=56.041
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=876
PRIMER_PAIR_41_PENALTY=5.342539
PRIMER_LEFT_41_PENALTY=1.708500
PRIMER_RIGHT_41_PENALTY=3.634039
PRIMER_LEFT_41_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_41_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_41=821,21
PRIMER_RIGHT_41=1697,23
PRIMER_LEFT_41_TM=60.708
PRIMER_RIGHT_41_TM=59.366
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=43.478
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=877
PRIMER_PAIR_42_PENALTY=5.348203
PRIMER_LEFT_42_PENALTY=1.116239
PRIMER_RIGHT_42_PENALTY=4.231965
PRIMER_LEFT_42_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_42_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_42=822,20
PRIMER_RIGHT_42=1698,23
PRIMER_LEFT_42_TM=58.884
PRIMER_RIGHT_42_TM=61.232
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=877
PRIMER_PAIR_43_PENALTY=5.431331
PRIMER_LEFT_43_PENALTY=1.199367
PRIMER_RIGHT_43_PENALTY=4.231965
PRIMER_LEFT_43_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_43_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_43=704,20
PRIMER_RIGHT_43=1698,23
PRIMER_LEFT_43_TM=61.199
PRIMER_RIGHT_43_TM=61.232
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=43.478
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=995
PRIMER_PAIR_44_PENALTY=5.491187
PRIMER_LEFT_44_PENALTY=1.259222
PRIMER_RIGHT_44_PENALTY=4.231965
PRIMER_LEFT_44_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_44_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_44=820,20
PRIMER_RIGHT_44=1698,23
PRIMER_LEFT_44_TM=58.741
PRIMER_RIGHT_44_TM=61.232
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=43.478
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=879
PRIMER_PAIR_45_PENALTY=5.523571
PRIMER_LEFT_45_PENALTY=1.584033
PRIMER_RIGHT_45_PENALTY=3.939538
PRIMER_LEFT_45_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_45_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_45=820,21
PRIMER_RIGHT_45=1695,21
PRIMER_LEFT_45_TM=60.584
PRIMER_RIGHT_45_TM=57.060
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=876
PRIMER_PAIR_46_PENALTY=5.543466
PRIMER_LEFT_46_PENALTY=1.584033
PRIMER_RIGHT_46_PENALTY=3.959434
PRIMER_LEFT_46_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_46_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_46=820,21
PRIMER_RIGHT_46=1695,20
PRIMER_LEFT_46_TM=60.584
PRIMER_RIGHT_46_TM=56.041
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=876
PRIMER_PAIR_47_PENALTY=5.576515
PRIMER_LEFT_47_PENALTY=2.202284
PRIMER_RIGHT_47_PENALTY=3.374230
PRIMER_LEFT_47_SEQUENCE=GATTACGGAGGAGGAAGAGG
PRIMER_RIGHT_47_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_47=823,20
PRIMER_RIGHT_47=1696,21
PRIMER_LEFT_47_TM=57.798
PRIMER_RIGHT_47_TM=57.626
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=874
PRIMER_PAIR_48_PENALTY=5.605008
PRIMER_LEFT_48_PENALTY=0.182658
PRIMER_RIGHT_48_PENALTY=5.422350
PRIMER_LEFT_48_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_48_SEQUENCE=ATGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_48=703,20
PRIMER_RIGHT_48=1699,24
PRIMER_LEFT_48_TM=60.183
PRIMER_RIGHT_48_TM=61.422
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=41.667
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=997
PRIMER_PAIR_49_PENALTY=5.648038
PRIMER_LEFT_49_PENALTY=1.708500
PRIMER_RIGHT_49_PENALTY=3.939538
PRIMER_LEFT_49_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_49_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_49=821,21
PRIMER_RIGHT_49=1695,21
PRIMER_LEFT_49_TM=60.708
PRIMER_RIGHT_49_TM=57.060
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=875
=
SEQUENCE_ID=MAGIC.105_4_17285093_17285105_can-0_col-0_484_2674_TRUE
SEQUENCE_TARGET=950,112
SEQUENCE_TEMPLATE=TAATAGGCATCAGAGTAGTGCATCNGAGATNTTGCAAAGTGTCACATTGGATNNNNNNAAGTCATTGGAAGCGGGAAAAAGTCCATGCATTGATACACTATTNACGTTGCATTTTAGGCCTTTCAAAAAGAAGNATAAGTNTAGTAGCACACCTGAGCAGATTTGTTTAGTTGAAGGAGGGTACAGAATCTTGGCATATGAACTGATATAGAGTTACTGTTGATGGGTTGACTTAGTTGTAGAGCTTCTCCTAAAATCTTGATGTACTTCCCAATTTCCTTCTGCTGTACATTGGCTGCAATGGAGATTTCCTATTGTGCAAAGNAAGACAAATACATTAGACACAAATCAAGTCTCAATAATAAACTGAAAGAAGTGCTGCTCCTGTATAAGTTCATAAGTGATGGTCCACATTTGGTTGTAACAAAATCTGCCTCTAGAAAAGATAATTCTGTATTCTACGATANGATATCCATNTNAAAGCTAATTTACGAACAAAACATGTCAAAAATATCANCAAACAACNGAGACAAATGATAAGAACAGTTATNNNNNNNNNTAAAGTACACCAACATGGAAGCTTNATTCCTTCAAGNAAACACACAAGCTTNAGAAACCTAGNATAGACTGGTNNTANTTCTTCAATTCGAAAACNNNGNNAGCNGTTCAAGAAANTACANTGATNANTTATCCAGAATNAACCANATAGTCACAACTTCTTTCAACNCAAAACGATGAGAACGAANTNGNTTTNTAACATAAACNNNNCATCTAGCAATGTAACTTCAANNNATTGAGNCTNANNAGCTNAATCTGCCATTANAACTTGTAATGANATAGGTTCTCTAATTACCTGAAGAGTTCTAGGTTCTTGAGCCTCCCTAATAGCTTGAACAAGGCAAGCAGTGGCTAAAGCTTCAACGCTTCTGTTTCGCAAGCAAGTAGCTGAGCAACAATCCCTAAACAACTGGAAAGCATGCTCAGAGATATCGCAATCCAANCCTAAAATCGANGCNACATCGATAATCTGCATATAAGCCCTAAGATTATCCACCACAACAGTCGACGAATTCGAATTCGATGTTGACGAAGCGAGTTCTAGGGTTCGTTCGAGTTCAGCGAGATGACCGGAGAAAGAGAGGGAAGAGCGAGCGAANATNGGGCTTGGCTCGAGNGACCAGGTAGAGAAGGCGGTNATGAATCCGGTNGGNTCNAACGGATCTTCCTCGTCTTCAGGAGACGGTTGCGCCGCNNTTTGGAGATCNGAAGTNACNANNCAGAGNGGAGTGTCTTGGGCNCGGAGATGNAANAGGTGGTGGTTTTGCGTCTGNCGTTCTTCCATTACGCGACCGCAAGATGAGCACTCCGTTATTGATCTACCGGAGCTNGTGGTGGNACACCGTCCCTGCGCCGATGAACAGTACGGACACTTCATTTTTGNGTTTCTTTACNTTTNNNTTTTTTTTTTNTNNNCTCTCANGTGNGAGNGTGNTAAAGCTNTTTTATACTACAAGAGTAAACAAACCGCGNATTTTAGTTTAGTCAACAANTCTTAATTTNGTAATCNACNGTGGCNNNNNGTTGCCAATAACNACACAGATTCAAGAGTCCCAAGTTTTGAAGATATCAGANGAAGAAATATATCATAAGGGATCCATTGCTTTTTCGTGTAAAGTCATAAGGTTCCANTTGCTTCTACATATANTTATAAAAGTAGCTCTATATAATTACCANCGATAGAATGTNAAAAGAGCAATTTGANTTCGAANNNTGAATTNTNGTCCCANAATGAANTCTATCCATTTGGTAGTTCATCTTTTGGCTTCAGCANNNNNNNNNNNNNNNTTACCAATATCATTATCAGCTACTAGCTGCTGGCTTGTNNAGAATTCTCTTCAAGTTTCTTCCCAAATCCCTNGCACTCTGAGAACAAACCGANAAAGAAAAGAAAAAAGAGNNTAACACTCAGGTTAAGTCTATGAACAATTTAAGAGAAGAANTTAAAAGATNAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.413143
PRIMER_LEFT_0_PENALTY=0.216240
PRIMER_RIGHT_0_PENALTY=0.196903
PRIMER_LEFT_0_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_0_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_0=401,20
PRIMER_RIGHT_0=1234,20
PRIMER_LEFT_0_TM=60.216
PRIMER_RIGHT_0_TM=59.803
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=834
PRIMER_PAIR_1_PENALTY=0.449463
PRIMER_LEFT_1_PENALTY=0.216240
PRIMER_RIGHT_1_PENALTY=0.233224
PRIMER_LEFT_1_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_1_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_1=401,20
PRIMER_RIGHT_1=1152,20
PRIMER_LEFT_1_TM=60.216
PRIMER_RIGHT_1_TM=60.233
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=752
PRIMER_PAIR_2_PENALTY=0.526453
PRIMER_LEFT_2_PENALTY=0.329550
PRIMER_RIGHT_2_PENALTY=0.196903
PRIMER_LEFT_2_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_2_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_2=268,20
PRIMER_RIGHT_2=1234,20
PRIMER_LEFT_2_TM=59.670
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=967
PRIMER_PAIR_3_PENALTY=0.562774
PRIMER_LEFT_3_PENALTY=0.329550
PRIMER_RIGHT_3_PENALTY=0.233224
PRIMER_LEFT_3_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_3_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_3=268,20
PRIMER_RIGHT_3=1152,20
PRIMER_LEFT_3_TM=59.670
PRIMER_RIGHT_3_TM=60.233
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=885
PRIMER_PAIR_4_PENALTY=0.745100
PRIMER_LEFT_4_PENALTY=0.216240
PRIMER_RIGHT_4_PENALTY=0.528861
PRIMER_LEFT_4_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_4_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_4=401,20
PRIMER_RIGHT_4=1103,20
PRIMER_LEFT_4_TM=60.216
PRIMER_RIGHT_4_TM=60.529
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=703
PRIMER_PAIR_5_PENALTY=0.808456
PRIMER_LEFT_5_PENALTY=0.279595
PRIMER_RIGHT_5_PENALTY=0.528861
PRIMER_LEFT_5_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_5_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_5=104,20
PRIMER_RIGHT_5=1103,20
PRIMER_LEFT_5_TM=59.720
PRIMER_RIGHT_5_TM=60.529
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=1000
PRIMER_PAIR_6_PENALTY=0.858410
PRIMER_LEFT_6_PENALTY=0.329550
PRIMER_RIGHT_6_PENALTY=0.528861
PRIMER_LEFT_6_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_6_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_6=268,20
PRIMER_RIGHT_6=1103,20
PRIMER_LEFT_6_TM=59.670
PRIMER_RIGHT_6_TM=60.529
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=836
PRIMER_PAIR_7_PENALTY=0.883159
PRIMER_LEFT_7_PENALTY=0.686256
PRIMER_RIGHT_7_PENALTY=0.196903
PRIMER_LEFT_7_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_7_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_7=367,20
PRIMER_RIGHT_7=1234,20
PRIMER_LEFT_7_TM=59.314
PRIMER_RIGHT_7_TM=59.803
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=868
PRIMER_PAIR_8_PENALTY=0.919480
PRIMER_LEFT_8_PENALTY=0.686256
PRIMER_RIGHT_8_PENALTY=0.233224
PRIMER_LEFT_8_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_8_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_8=367,20
PRIMER_RIGHT_8=1152,20
PRIMER_LEFT_8_TM=59.314
PRIMER_RIGHT_8_TM=60.233
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=786
PRIMER_PAIR_9_PENALTY=0.920600
PRIMER_LEFT_9_PENALTY=0.216240
PRIMER_RIGHT_9_PENALTY=0.704360
PRIMER_LEFT_9_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_9_SEQUENCE=TTCCCTCTCTTTCTCCGGTC
PRIMER_LEFT_9=401,20
PRIMER_RIGHT_9=1144,20
PRIMER_LEFT_9_TM=60.216
PRIMER_RIGHT_9_TM=60.704
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=744
PRIMER_PAIR_10_PENALTY=1.033910
PRIMER_LEFT_10_PENALTY=0.329550
PRIMER_RIGHT_10_PENALTY=0.704360
PRIMER_LEFT_10_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_10_SEQUENCE=TTCCCTCTCTTTCTCCGGTC
PRIMER_LEFT_10=268,20
PRIMER_RIGHT_10=1144,20
PRIMER_LEFT_10_TM=59.670
PRIMER_RIGHT_10_TM=60.704
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=2.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=877
PRIMER_PAIR_11_PENALTY=1.038279
PRIMER_LEFT_11_PENALTY=0.580558
PRIMER_RIGHT_11_PENALTY=0.457721
PRIMER_LEFT_11_SEQUENCE=GCTTGAACAAGGCAAGCAGT
PRIMER_RIGHT_11_SEQUENCE=TGCTGAAGCCAAAAGATGAA
PRIMER_LEFT_11=887,20
PRIMER_RIGHT_11=1829,20
PRIMER_LEFT_11_TM=60.581
PRIMER_RIGHT_11_TM=59.542
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=943
PRIMER_PAIR_12_PENALTY=1.099923
PRIMER_LEFT_12_PENALTY=0.216240
PRIMER_RIGHT_12_PENALTY=0.883683
PRIMER_LEFT_12_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_12_SEQUENCE=TCATCTCGCTGAACTCGAAC
PRIMER_LEFT_12=401,20
PRIMER_RIGHT_12=1126,20
PRIMER_LEFT_12_TM=60.216
PRIMER_RIGHT_12_TM=59.116
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=726
PRIMER_PAIR_13_PENALTY=1.167512
PRIMER_LEFT_13_PENALTY=0.709791
PRIMER_RIGHT_13_PENALTY=0.457721
PRIMER_LEFT_13_SEQUENCE=AGCTTGAACAAGGCAAGCAG
PRIMER_RIGHT_13_SEQUENCE=TGCTGAAGCCAAAAGATGAA
PRIMER_LEFT_13=886,20
PRIMER_RIGHT_13=1829,20
PRIMER_LEFT_13_TM=60.710
PRIMER_RIGHT_13_TM=59.542
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=944
PRIMER_PAIR_14_PENALTY=1.213233
PRIMER_LEFT_14_PENALTY=0.329550
PRIMER_RIGHT_14_PENALTY=0.883683
PRIMER_LEFT_14_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_14_SEQUENCE=TCATCTCGCTGAACTCGAAC
PRIMER_LEFT_14=268,20
PRIMER_RIGHT_14=1126,20
PRIMER_LEFT_14_TM=59.670
PRIMER_RIGHT_14_TM=59.116
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=859
PRIMER_PAIR_15_PENALTY=1.215116
PRIMER_LEFT_15_PENALTY=0.686256
PRIMER_RIGHT_15_PENALTY=0.528861
PRIMER_LEFT_15_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_15_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_15=367,20
PRIMER_RIGHT_15=1103,20
PRIMER_LEFT_15_TM=59.314
PRIMER_RIGHT_15_TM=60.529
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=737
PRIMER_PAIR_16_PENALTY=1.228129
PRIMER_LEFT_16_PENALTY=0.216240
PRIMER_RIGHT_16_PENALTY=1.011889
PRIMER_LEFT_16_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_16_SEQUENCE=GTCATCTCGCTGAACTCGAAC
PRIMER_LEFT_16=401,20
PRIMER_RIGHT_16=1127,21
PRIMER_LEFT_16_TM=60.216
PRIMER_RIGHT_16_TM=60.012
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=727
PRIMER_PAIR_17_PENALTY=1.307002
PRIMER_LEFT_17_PENALTY=1.110099
PRIMER_RIGHT_17_PENALTY=0.196903
PRIMER_LEFT_17_SEQUENCE=TCCTTCTGCTGTACATTGGCT
PRIMER_RIGHT_17_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_17=276,21
PRIMER_RIGHT_17=1234,20
PRIMER_LEFT_17_TM=59.890
PRIMER_RIGHT_17_TM=59.803
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=959
PRIMER_PAIR_18_PENALTY=1.316833
PRIMER_LEFT_18_PENALTY=0.216240
PRIMER_RIGHT_18_PENALTY=1.100593
PRIMER_LEFT_18_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_18_SEQUENCE=AACCCTAGAACTCGCTTCGTC
PRIMER_LEFT_18=401,20
PRIMER_RIGHT_18=1105,21
PRIMER_LEFT_18_TM=60.216
PRIMER_RIGHT_18_TM=59.899
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=705
PRIMER_PAIR_19_PENALTY=1.316833
PRIMER_LEFT_19_PENALTY=0.216240
PRIMER_RIGHT_19_PENALTY=1.100593
PRIMER_LEFT_19_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_19_SEQUENCE=GAACCCTAGAACTCGCTTCGT
PRIMER_LEFT_19=401,20
PRIMER_RIGHT_19=1106,21
PRIMER_LEFT_19_TM=60.216
PRIMER_RIGHT_19_TM=59.899
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=706
PRIMER_PAIR_20_PENALTY=1.332205
PRIMER_LEFT_20_PENALTY=0.216240
PRIMER_RIGHT_20_PENALTY=1.115965
PRIMER_LEFT_20_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_20_SEQUENCE=CTCGCTGAACTCGAACGAAC
PRIMER_LEFT_20=401,20
PRIMER_RIGHT_20=1122,20
PRIMER_LEFT_20_TM=60.216
PRIMER_RIGHT_20_TM=61.116
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=722
PRIMER_PAIR_21_PENALTY=1.341439
PRIMER_LEFT_21_PENALTY=0.329550
PRIMER_RIGHT_21_PENALTY=1.011889
PRIMER_LEFT_21_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_21_SEQUENCE=GTCATCTCGCTGAACTCGAAC
PRIMER_LEFT_21=268,20
PRIMER_RIGHT_21=1127,21
PRIMER_LEFT_21_TM=59.670
PRIMER_RIGHT_21_TM=60.012
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=860
PRIMER_PAIR_22_PENALTY=1.343323
PRIMER_LEFT_22_PENALTY=1.110099
PRIMER_RIGHT_22_PENALTY=0.233224
PRIMER_LEFT_22_SEQUENCE=TCCTTCTGCTGTACATTGGCT
PRIMER_RIGHT_22_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_22=276,21
PRIMER_RIGHT_22=1152,20
PRIMER_LEFT_22_TM=59.890
PRIMER_RIGHT_22_TM=60.233
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=877
PRIMER_PAIR_23_PENALTY=1.390616
PRIMER_LEFT_23_PENALTY=0.686256
PRIMER_RIGHT_23_PENALTY=0.704360
PRIMER_LEFT_23_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_23_SEQUENCE=TTCCCTCTCTTTCTCCGGTC
PRIMER_LEFT_23=367,20
PRIMER_RIGHT_23=1144,20
PRIMER_LEFT_23_TM=59.314
PRIMER_RIGHT_23_TM=60.704
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=778
PRIMER_PAIR_24_PENALTY=1.390749
PRIMER_LEFT_24_PENALTY=0.216240
PRIMER_RIGHT_24_PENALTY=1.174509
PRIMER_LEFT_24_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_24_SEQUENCE=CCTCTCTTTCTCCGGTCATCT
PRIMER_LEFT_24=401,20
PRIMER_RIGHT_24=1141,21
PRIMER_LEFT_24_TM=60.216
PRIMER_RIGHT_24_TM=59.825
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=741
PRIMER_PAIR_25_PENALTY=1.400328
PRIMER_LEFT_25_PENALTY=0.216240
PRIMER_RIGHT_25_PENALTY=1.184089
PRIMER_LEFT_25_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_25_SEQUENCE=CCTCTCTTTCTCCGGTCATC
PRIMER_LEFT_25=401,20
PRIMER_RIGHT_25=1141,20
PRIMER_LEFT_25_TM=60.216
PRIMER_RIGHT_25_TM=58.816
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=741
PRIMER_PAIR_26_PENALTY=1.421430
PRIMER_LEFT_26_PENALTY=0.963709
PRIMER_RIGHT_26_PENALTY=0.457721
PRIMER_LEFT_26_SEQUENCE=ACAAGGCAAGCAGTGGCTAA
PRIMER_RIGHT_26_SEQUENCE=TGCTGAAGCCAAAAGATGAA
PRIMER_LEFT_26=893,20
PRIMER_RIGHT_26=1829,20
PRIMER_LEFT_26_TM=60.964
PRIMER_RIGHT_26_TM=59.542
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.5000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=937
PRIMER_PAIR_27_PENALTY=1.430143
PRIMER_LEFT_27_PENALTY=0.329550
PRIMER_RIGHT_27_PENALTY=1.100593
PRIMER_LEFT_27_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_27_SEQUENCE=AACCCTAGAACTCGCTTCGTC
PRIMER_LEFT_27=268,20
PRIMER_RIGHT_27=1105,21
PRIMER_LEFT_27_TM=59.670
PRIMER_RIGHT_27_TM=59.899
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=8.1000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=838
PRIMER_PAIR_28_PENALTY=1.430143
PRIMER_LEFT_28_PENALTY=0.329550
PRIMER_RIGHT_28_PENALTY=1.100593
PRIMER_LEFT_28_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_28_SEQUENCE=GAACCCTAGAACTCGCTTCGT
PRIMER_LEFT_28=268,20
PRIMER_RIGHT_28=1106,21
PRIMER_LEFT_28_TM=59.670
PRIMER_RIGHT_28_TM=59.899
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=8.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=839
PRIMER_PAIR_29_PENALTY=1.434901
PRIMER_LEFT_29_PENALTY=0.216240
PRIMER_RIGHT_29_PENALTY=1.218662
PRIMER_LEFT_29_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_29_SEQUENCE=TCGCTCTTCCCTCTCTTTCTC
PRIMER_LEFT_29=401,20
PRIMER_RIGHT_29=1150,21
PRIMER_LEFT_29_TM=60.216
PRIMER_RIGHT_29_TM=60.219
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=750
PRIMER_PAIR_30_PENALTY=1.445515
PRIMER_LEFT_30_PENALTY=0.329550
PRIMER_RIGHT_30_PENALTY=1.115965
PRIMER_LEFT_30_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_30_SEQUENCE=CTCGCTGAACTCGAACGAAC
PRIMER_LEFT_30=268,20
PRIMER_RIGHT_30=1122,20
PRIMER_LEFT_30_TM=59.670
PRIMER_RIGHT_30_TM=61.116
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=855
PRIMER_PAIR_31_PENALTY=1.504059
PRIMER_LEFT_31_PENALTY=0.329550
PRIMER_RIGHT_31_PENALTY=1.174509
PRIMER_LEFT_31_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_31_SEQUENCE=CCTCTCTTTCTCCGGTCATCT
PRIMER_LEFT_31=268,20
PRIMER_RIGHT_31=1141,21
PRIMER_LEFT_31_TM=59.670
PRIMER_RIGHT_31_TM=59.825
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=874
PRIMER_PAIR_32_PENALTY=1.513638
PRIMER_LEFT_32_PENALTY=0.329550
PRIMER_RIGHT_32_PENALTY=1.184089
PRIMER_LEFT_32_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_32_SEQUENCE=CCTCTCTTTCTCCGGTCATC
PRIMER_LEFT_32=268,20
PRIMER_RIGHT_32=1141,20
PRIMER_LEFT_32_TM=59.670
PRIMER_RIGHT_32_TM=58.816
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=6.6000
PRIMER_PAIR_32_COMPL_ANY=2.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=874
PRIMER_PAIR_33_PENALTY=1.516132
PRIMER_LEFT_33_PENALTY=1.319229
PRIMER_RIGHT_33_PENALTY=0.196903
PRIMER_LEFT_33_SEQUENCE=CTGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_33_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_33=366,21
PRIMER_RIGHT_33=1234,20
PRIMER_LEFT_33_TM=60.319
PRIMER_RIGHT_33_TM=59.803
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=869
PRIMER_PAIR_34_PENALTY=1.548212
PRIMER_LEFT_34_PENALTY=0.329550
PRIMER_RIGHT_34_PENALTY=1.218662
PRIMER_LEFT_34_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_34_SEQUENCE=TCGCTCTTCCCTCTCTTTCTC
PRIMER_LEFT_34=268,20
PRIMER_RIGHT_34=1150,21
PRIMER_LEFT_34_TM=59.670
PRIMER_RIGHT_34_TM=60.219
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=2.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=883
PRIMER_PAIR_35_PENALTY=1.552453
PRIMER_LEFT_35_PENALTY=1.319229
PRIMER_RIGHT_35_PENALTY=0.233224
PRIMER_LEFT_35_SEQUENCE=CTGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_35_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_35=366,21
PRIMER_RIGHT_35=1152,20
PRIMER_LEFT_35_TM=60.319
PRIMER_RIGHT_35_TM=60.233
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=787
PRIMER_PAIR_36_PENALTY=1.569939
PRIMER_LEFT_36_PENALTY=0.686256
PRIMER_RIGHT_36_PENALTY=0.883683
PRIMER_LEFT_36_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_36_SEQUENCE=TCATCTCGCTGAACTCGAAC
PRIMER_LEFT_36=367,20
PRIMER_RIGHT_36=1126,20
PRIMER_LEFT_36_TM=59.314
PRIMER_RIGHT_36_TM=59.116
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=8.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=760
PRIMER_PAIR_37_PENALTY=1.623208
PRIMER_LEFT_37_PENALTY=1.165486
PRIMER_RIGHT_37_PENALTY=0.457721
PRIMER_LEFT_37_SEQUENCE=TTCTAGGTTCTTGAGCCTCCC
PRIMER_RIGHT_37_SEQUENCE=TGCTGAAGCCAAAAGATGAA
PRIMER_LEFT_37=861,21
PRIMER_RIGHT_37=1829,20
PRIMER_LEFT_37_TM=59.835
PRIMER_RIGHT_37_TM=59.542
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=969
PRIMER_PAIR_38_PENALTY=1.638960
PRIMER_LEFT_38_PENALTY=1.110099
PRIMER_RIGHT_38_PENALTY=0.528861
PRIMER_LEFT_38_SEQUENCE=TCCTTCTGCTGTACATTGGCT
PRIMER_RIGHT_38_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_38=276,21
PRIMER_RIGHT_38=1103,20
PRIMER_LEFT_38_TM=59.890
PRIMER_RIGHT_38_TM=60.529
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=828
PRIMER_PAIR_39_PENALTY=1.665148
PRIMER_LEFT_39_PENALTY=0.216240
PRIMER_RIGHT_39_PENALTY=1.448909
PRIMER_LEFT_39_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_39_SEQUENCE=ACCCTAGAACTCGCTTCGTC
PRIMER_LEFT_39=401,20
PRIMER_RIGHT_39=1104,20
PRIMER_LEFT_39_TM=60.216
PRIMER_RIGHT_39_TM=58.551
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=704
PRIMER_PAIR_40_PENALTY=1.698145
PRIMER_LEFT_40_PENALTY=0.686256
PRIMER_RIGHT_40_PENALTY=1.011889
PRIMER_LEFT_40_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_40_SEQUENCE=GTCATCTCGCTGAACTCGAAC
PRIMER_LEFT_40=367,20
PRIMER_RIGHT_40=1127,21
PRIMER_LEFT_40_TM=59.314
PRIMER_RIGHT_40_TM=60.012
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=761
PRIMER_PAIR_41_PENALTY=1.724200
PRIMER_LEFT_41_PENALTY=0.216240
PRIMER_RIGHT_41_PENALTY=1.507961
PRIMER_LEFT_41_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_41_SEQUENCE=AGAACTCGCTTCGTCAACATC
PRIMER_LEFT_41=401,20
PRIMER_RIGHT_41=1099,21
PRIMER_LEFT_41_TM=60.216
PRIMER_RIGHT_41_TM=59.492
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.3000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=699
PRIMER_PAIR_42_PENALTY=1.772427
PRIMER_LEFT_42_PENALTY=0.216240
PRIMER_RIGHT_42_PENALTY=1.556187
PRIMER_LEFT_42_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_42_SEQUENCE=GAACTCGCTTCGTCAACATC
PRIMER_LEFT_42=401,20
PRIMER_RIGHT_42=1098,20
PRIMER_LEFT_42_TM=60.216
PRIMER_RIGHT_42_TM=58.444
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=698
PRIMER_PAIR_43_PENALTY=1.778458
PRIMER_LEFT_43_PENALTY=0.329550
PRIMER_RIGHT_43_PENALTY=1.448909
PRIMER_LEFT_43_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_43_SEQUENCE=ACCCTAGAACTCGCTTCGTC
PRIMER_LEFT_43=268,20
PRIMER_RIGHT_43=1104,20
PRIMER_LEFT_43_TM=59.670
PRIMER_RIGHT_43_TM=58.551
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=8.1000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=837
PRIMER_PAIR_44_PENALTY=1.786849
PRIMER_LEFT_44_PENALTY=0.686256
PRIMER_RIGHT_44_PENALTY=1.100593
PRIMER_LEFT_44_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_44_SEQUENCE=AACCCTAGAACTCGCTTCGTC
PRIMER_LEFT_44=367,20
PRIMER_RIGHT_44=1105,21
PRIMER_LEFT_44_TM=59.314
PRIMER_RIGHT_44_TM=59.899
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=739
PRIMER_PAIR_45_PENALTY=1.786849
PRIMER_LEFT_45_PENALTY=0.686256
PRIMER_RIGHT_45_PENALTY=1.100593
PRIMER_LEFT_45_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_45_SEQUENCE=GAACCCTAGAACTCGCTTCGT
PRIMER_LEFT_45=367,20
PRIMER_RIGHT_45=1106,21
PRIMER_LEFT_45_TM=59.314
PRIMER_RIGHT_45_TM=59.899
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=740
PRIMER_PAIR_46_PENALTY=1.787556
PRIMER_LEFT_46_PENALTY=0.279595
PRIMER_RIGHT_46_PENALTY=1.507961
PRIMER_LEFT_46_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_46_SEQUENCE=AGAACTCGCTTCGTCAACATC
PRIMER_LEFT_46=104,20
PRIMER_RIGHT_46=1099,21
PRIMER_LEFT_46_TM=59.720
PRIMER_RIGHT_46_TM=59.492
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.3000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=996
PRIMER_PAIR_47_PENALTY=1.802221
PRIMER_LEFT_47_PENALTY=0.686256
PRIMER_RIGHT_47_PENALTY=1.115965
PRIMER_LEFT_47_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_47_SEQUENCE=CTCGCTGAACTCGAACGAAC
PRIMER_LEFT_47=367,20
PRIMER_RIGHT_47=1122,20
PRIMER_LEFT_47_TM=59.314
PRIMER_RIGHT_47_TM=61.116
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=756
PRIMER_PAIR_48_PENALTY=1.812086
PRIMER_LEFT_48_PENALTY=1.615183
PRIMER_RIGHT_48_PENALTY=0.196903
PRIMER_LEFT_48_SEQUENCE=CTTCCCAATTTCCTTCTGCT
PRIMER_RIGHT_48_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_48=266,20
PRIMER_RIGHT_48=1234,20
PRIMER_LEFT_48_TM=58.385
PRIMER_RIGHT_48_TM=59.803
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=969
PRIMER_PAIR_49_PENALTY=1.814459
PRIMER_LEFT_49_PENALTY=1.110099
PRIMER_RIGHT_49_PENALTY=0.704360
PRIMER_LEFT_49_SEQUENCE=TCCTTCTGCTGTACATTGGCT
PRIMER_RIGHT_49_SEQUENCE=TTCCCTCTCTTTCTCCGGTC
PRIMER_LEFT_49=276,21
PRIMER_RIGHT_49=1144,20
PRIMER_LEFT_49_TM=59.890
PRIMER_RIGHT_49_TM=60.704
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=869
=
SEQUENCE_ID=MAGIC.338_3_16650699_16650930_no-0_kn-0_307549_388892_TRUE
SEQUENCE_TARGET=950,331
SEQUENCE_TEMPLATE=NTGAGTAAAGAAGGAGAGAGACTGAGATGAAGCTGAACCGAANTCGAATGGNNGGTTGAAGAAGGCATGACCCTTTTNNNNAGTCGTNCCATCGGTTAGNTGCAAAACTCTTTCTGGAGATTGNATTCTTGCNAANCCATCAAGGAGAAGTTCATCACCAGCTTGGTCTTGGTNAAAGCCATTGAAGANGAAACTTAANTCTTGNTGACTTGAAAAGCAAATNAGATTAACTAAGAAGATCAAGAGNAGAAGNAGAANANAAAATCTTTGAGCCATTGGNATTGAAAAAANCAGAGACANGNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNAGAAATATTCTCTNNANGCAAGAAAGAGTTGANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTTAACAATGTCNAGTCNGAACTAAATTAAAGNNAAATACCCTCNNAGCGTTTACCAATTACNATTATTNGTTTAAGACAACCTTGAAAAGANAGACTNTGCATCNTTNTCAATATCGTTTAGCTCTAAAACAGCTATACAACTTTGGAGACGAAGGTGTGTTGAGTNCCGAGGNCATGNGGANGAGAAGAGANGATAACGTGCTNNNNNNNNNTCCCGTNAAANACCNTACTTTGTCAANCCCATTTCTTGCTCTATNCACGAACCGGTTCANNTTAANTGATCGANNTCACGAAATGATTTGTACTTTTGTTCTTNACGAAAAATAAATGGATGATNNNNACGCAAAAAAGGNAAGCAGATNAATCTCAACCCTGACAGAAACATGAGNCAGTGTAGAAAANATCATGNAGTCANANTTTNTCCNGTGCCTGATTAGCCTCCATAGATCTTCTAANTCCTTTGATCCNNTTNAGTATAAAGTGNCCTTATACTCTNTGNTTTAANNTTTTTGGGGTANNNNNNNNNNNNNNNNNNNNGGAAATAAAGNTCTTTTAGTTCAAGATGGTGTNTGATNAGAAATAACCTTTTTGGAGNAAGGTTTTATCACTTTTTAGTTCTAAGTGTTGNNNNNNNCTGGGTCAAAATTTGGAGATTTCCATGAAGTCCTCAAAAGTTCTACTAATAGTCTATGGTCTACTGATAAGATGGTGAGCNAGNAGCNGTCATGACATATGNATAAGATCAGATTCGAAACTTNGGTTTTTCGNTTTTCCGTTCAGCTTTTCACAATTGCTCCCATGTTCGNACTTCTCCTTTAGTAGATGAACATTTTGTACCTTTCGCTTTATTTNCTTGCTTGTAAAGTNAATGTTTTCTCTCTTTACTTAGNCTTCNTTGNTTTGTAAAGTTATCTACGTGTATCATTTCTTGTACNTGTACATGCCTNTCCANAATGNTTNNCCATNGATNGATGATGNGCTTGTNNNNATANANATANCAGTCACTCTTGNAAAANANAAANANNTGTGGCTCTATGTATGATTGATCAANTAAAATGGGAAGAACAAAAGGAAAATAAGACACAAAAAGAAAGAAAAAAATGTTGATTGAAATTGAAGGAGTAATTTTCAAAGTCCCAATNATANATGTCTCACCATTACAATAGAGAAACATTTCATTAGTGGCCTCCNAANAAATTTCTTACANAATNTTTTTTGTTATTAATGTTNANAAANNNNNNNAAAAAAAGAGATTAATTATTNGTTTTCTTTTTATAGTAACTACTAGTTAAANTATTTTGTTTCTTAAAGTAAACTATTCTNNCNTTTTTTTTGNGACNNTGTGTCTACAAAAAGTNGTGNNAAAAAAAAAGAAACNNAAAAATCNNNAAGTAATTGGAATATAAATTTGATNACAAAAGACNATATACATAAAAACAACTAAACAAGTATAACAAATCCATAAATACGAATTAAAAATNAAAGAGTTAATTATAGAAAACTTTCCGATCTCTGAAGCTTCAACACCGTTTGTTCNTCCTTGTTCTCCGCCGCATTGTCTCTTTCCATGAGAGATCAATTTCTTCACAAAGAAGCTTCTTCTTCTTCTACTACATTCCTCTNAGCACCAATCTCTCTTTCGCATCTCAATTCGAAACGTGAGTATTTCCTTTTTCTCCATTCTCNAATCGAGCANAAGTCGAGTCTCGCAACANCACTTAACCTAGAATTGATGATTCGNTNTACAATTTTCCGGTGTGTATCGAGCTGATTCTCTGATGTAGAAGCCCTAATTTNTNTGNNTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_5211102_5211361_can-0_po-0_12718_198400_TRUE
SEQUENCE_TARGET=950,359
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACATAATAGACANATATACTCTCTTTTCTNCTAAAACACNTNAAANTAATGGCTCTTGAAGACTAATGGTTACGTCACCATAGAGGAACANNNGAAGTGNATNGATGGCAGTCTGGTCTAGACCTGAGTCNTGTNGGNTACAAATCAAAGGTCCCACATCGNAAGTTTAGAAAAANANNTTCTAATATATAANGCAAGTNCANCTCCAACTAGTATAAGNCCTTGGNAAGAAGTNCAATAACAAATCNTTNCNGGNNNNNNNNNNNNNNNNNNNAGAGGACAATGTCATACTAAATGGAAGTTGGAATGGTCTTAGAAAAGCNATTAAATGGTATNAGAGCCTAGGTTGAAAAAATGGACCTANGGCTTAGGNTATGATCNNTGTNGGAGAATTTNTCAAACATGACANGATAAGAAGGTGCCTCAAGAATTTCACGAATAAGAAGGTGCCAAGTTAAGGGTGCNAAAGTTTCGTNGAAGAGGAGACCCANGCTCGTGGTCTTTGGTTTGAAGGGNAGATTGTTTGGTANAAAGTAAAGTTCTCANATCGGAAGTTTAGAAAAAGAGTTTCTNATATATAANCCAAGTCCAANTCCAACTNGTATGANGCCTNGGGAAGGAGNCCAATAACAAATCCTTGCANGCTTGAGCATGTAAGCCCNAAAANGACAATATCATACTNAATGGNAGTTGGAATGGGCTTNGAAAGCCCATTAAGTCATGGAGGAAGAAGAGGTGATGGAGCTGAGGTGAGGATGAAGAAANGGGGAANNGNAAATAAAGTCGANGAGAAANTTTGATATCAAATAGAGGATACCNTGGAAGACAATATGATAAGAGCAAGAACANAGATAACTAAACATAAAACAGAGANTGTAGAGCTTGAANATGGATCTNTCTNGNTNATAAGTGTNATTNTTGTTGCAGATGAAGATTTTGCTTTNTAAGATGTAAGAGAGAAATNTAAGCAGAACGATGAACATTAAATTCNCATGTTTCTCTTGCTCNCTCTGTTTGGTTAAAAANAGTTCAANTGTNTGGNTAGAGATNCANAGAAGCTAACTTTAGTCATCNGCAAAGATTATCNAAAAAACATCTTAANATAAAACATATGAATAGNCATGNGATTGTTTGCTGTTANTTANACCCAAAAAAAGATGCAATAGATACTAACTAATTAGCCGTTCCAAAAGAAGATAAACNTGAAAGTCATAAAGGTTCCAACACAAAACCAAAAAGAAAAACATAGGCAAATGCATCTCTAAATAGATAAAGACTAAACATATTTAGCCATCAAAGANNNNNNNNNNNNNNNNNNNNNNNNNNTCTCCCACATCNTTTTNAGCTCATCAGCTTGCACGGACTTCACCTTTTCTTCTATCCTNTTCCTCTTCTTCATNGGTACCAANCTCCAATTCTTCTTCCATGTCTCTTCATCAACCCCCNAATCCAATTCCTTAAACGCACCACCAACAAACGACCTCTCGAACCAATCACTTTCTTCATCAACTTAAGCTTNTTCCTCTTTGGCTTCNCAAGAGTAGCNACTCANTCTCAGTCTCCAACTCTTAATCTGAATTTTGATNGACAACANCAGTTTCTNGAGTCTTCTTACNAAAATCGTCTTCCTCCTCCTNCTCAGATGAAGAACNNGAGTTTTCTTCTTCGTCGCTANAAGACTCNGGCNGTGTAATTTTAACCTGTTTTCGTTGATTCTTCGAAGTTGATGCGACNACTTCCAACGGNNTCTTCGCCAGAGATGCGAANTCGATGNAGTAACCAAAGGCTGCNATTGCTTATTCTTCACCATNNGAATTATTGAAACNAGAGATAAATTTGAAANCTTNTANAATTTTAGGGTTTGTTATGAGAAGTTGNANNTGTANAATCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.581671
PRIMER_LEFT_0_PENALTY=0.519710
PRIMER_RIGHT_0_PENALTY=0.061961
PRIMER_LEFT_0_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_0_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_0=720,20
PRIMER_RIGHT_0=1693,20
PRIMER_LEFT_0_TM=60.520
PRIMER_RIGHT_0_TM=59.938
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=974
PRIMER_PAIR_1_PENALTY=0.637316
PRIMER_LEFT_1_PENALTY=0.575355
PRIMER_RIGHT_1_PENALTY=0.061961
PRIMER_LEFT_1_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_1_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_1=721,20
PRIMER_RIGHT_1=1693,20
PRIMER_LEFT_1_TM=59.425
PRIMER_RIGHT_1_TM=59.938
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=1.045357
PRIMER_LEFT_2_PENALTY=0.519710
PRIMER_RIGHT_2_PENALTY=0.525647
PRIMER_LEFT_2_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_2_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_2=720,20
PRIMER_RIGHT_2=1710,20
PRIMER_LEFT_2_TM=60.520
PRIMER_RIGHT_2_TM=59.474
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=991
PRIMER_PAIR_3_PENALTY=1.064841
PRIMER_LEFT_3_PENALTY=0.519710
PRIMER_RIGHT_3_PENALTY=0.545130
PRIMER_LEFT_3_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_3_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_3=720,20
PRIMER_RIGHT_3=1711,20
PRIMER_LEFT_3_TM=60.520
PRIMER_RIGHT_3_TM=60.545
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=992
PRIMER_PAIR_4_PENALTY=1.101002
PRIMER_LEFT_4_PENALTY=0.575355
PRIMER_RIGHT_4_PENALTY=0.525647
PRIMER_LEFT_4_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_4_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_4=721,20
PRIMER_RIGHT_4=1710,20
PRIMER_LEFT_4_TM=59.425
PRIMER_RIGHT_4_TM=59.474
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=990
PRIMER_PAIR_5_PENALTY=1.120486
PRIMER_LEFT_5_PENALTY=0.575355
PRIMER_RIGHT_5_PENALTY=0.545130
PRIMER_LEFT_5_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_5_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_5=721,20
PRIMER_RIGHT_5=1711,20
PRIMER_LEFT_5_TM=59.425
PRIMER_RIGHT_5_TM=60.545
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=991
PRIMER_PAIR_6_PENALTY=1.209391
PRIMER_LEFT_6_PENALTY=0.519710
PRIMER_RIGHT_6_PENALTY=0.689681
PRIMER_LEFT_6_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_6_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_6=720,20
PRIMER_RIGHT_6=1713,20
PRIMER_LEFT_6_TM=60.520
PRIMER_RIGHT_6_TM=60.690
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=994
PRIMER_PAIR_7_PENALTY=1.209391
PRIMER_LEFT_7_PENALTY=0.519710
PRIMER_RIGHT_7_PENALTY=0.689681
PRIMER_LEFT_7_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_7_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_7=720,20
PRIMER_RIGHT_7=1717,20
PRIMER_LEFT_7_TM=60.520
PRIMER_RIGHT_7_TM=60.690
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=998
PRIMER_PAIR_8_PENALTY=1.265036
PRIMER_LEFT_8_PENALTY=0.575355
PRIMER_RIGHT_8_PENALTY=0.689681
PRIMER_LEFT_8_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_8_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_8=721,20
PRIMER_RIGHT_8=1713,20
PRIMER_LEFT_8_TM=59.425
PRIMER_RIGHT_8_TM=60.690
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=993
PRIMER_PAIR_9_PENALTY=1.265036
PRIMER_LEFT_9_PENALTY=0.575355
PRIMER_RIGHT_9_PENALTY=0.689681
PRIMER_LEFT_9_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_9_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_9=721,20
PRIMER_RIGHT_9=1717,20
PRIMER_LEFT_9_TM=59.425
PRIMER_RIGHT_9_TM=60.690
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=997
PRIMER_PAIR_10_PENALTY=1.277948
PRIMER_LEFT_10_PENALTY=1.215987
PRIMER_RIGHT_10_PENALTY=0.061961
PRIMER_LEFT_10_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_10_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_10=717,20
PRIMER_RIGHT_10=1693,20
PRIMER_LEFT_10_TM=61.216
PRIMER_RIGHT_10_TM=59.938
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=977
PRIMER_PAIR_11_PENALTY=1.302355
PRIMER_LEFT_11_PENALTY=1.240394
PRIMER_RIGHT_11_PENALTY=0.061961
PRIMER_LEFT_11_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_11_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_11=718,21
PRIMER_RIGHT_11=1693,20
PRIMER_LEFT_11_TM=59.760
PRIMER_RIGHT_11_TM=59.938
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=976
PRIMER_PAIR_12_PENALTY=1.315672
PRIMER_LEFT_12_PENALTY=1.253711
PRIMER_RIGHT_12_PENALTY=0.061961
PRIMER_LEFT_12_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_12_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_12=719,20
PRIMER_RIGHT_12=1693,20
PRIMER_LEFT_12_TM=58.746
PRIMER_RIGHT_12_TM=59.938
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=975
PRIMER_PAIR_13_PENALTY=1.332731
PRIMER_LEFT_13_PENALTY=0.519710
PRIMER_RIGHT_13_PENALTY=0.813021
PRIMER_LEFT_13_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_13_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_13=720,20
PRIMER_RIGHT_13=1716,20
PRIMER_LEFT_13_TM=60.520
PRIMER_RIGHT_13_TM=59.187
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=997
PRIMER_PAIR_14_PENALTY=1.375804
PRIMER_LEFT_14_PENALTY=0.519710
PRIMER_RIGHT_14_PENALTY=0.856094
PRIMER_LEFT_14_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_14_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_14=720,20
PRIMER_RIGHT_14=1694,20
PRIMER_LEFT_14_TM=60.520
PRIMER_RIGHT_14_TM=60.856
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=975
PRIMER_PAIR_15_PENALTY=1.388376
PRIMER_LEFT_15_PENALTY=0.575355
PRIMER_RIGHT_15_PENALTY=0.813021
PRIMER_LEFT_15_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_15_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_15=721,20
PRIMER_RIGHT_15=1716,20
PRIMER_LEFT_15_TM=59.425
PRIMER_RIGHT_15_TM=59.187
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=996
PRIMER_PAIR_16_PENALTY=1.431449
PRIMER_LEFT_16_PENALTY=0.575355
PRIMER_RIGHT_16_PENALTY=0.856094
PRIMER_LEFT_16_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_16_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_16=721,20
PRIMER_RIGHT_16=1694,20
PRIMER_LEFT_16_TM=59.425
PRIMER_RIGHT_16_TM=60.856
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=974
PRIMER_PAIR_17_PENALTY=1.434568
PRIMER_LEFT_17_PENALTY=0.575355
PRIMER_RIGHT_17_PENALTY=0.859212
PRIMER_LEFT_17_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_17_SEQUENCE=GATTGGTTCGAGAGGTCGTT
PRIMER_LEFT_17=721,20
PRIMER_RIGHT_17=1720,20
PRIMER_LEFT_17_TM=59.425
PRIMER_RIGHT_17_TM=59.141
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=1000
PRIMER_PAIR_18_PENALTY=1.443460
PRIMER_LEFT_18_PENALTY=0.519710
PRIMER_RIGHT_18_PENALTY=0.923750
PRIMER_LEFT_18_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_18_SEQUENCE=GTTGGTGGTGCGTTTAAGGA
PRIMER_LEFT_18=720,20
PRIMER_RIGHT_18=1699,20
PRIMER_LEFT_18_TM=60.520
PRIMER_RIGHT_18_TM=60.924
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=980
PRIMER_PAIR_19_PENALTY=1.499105
PRIMER_LEFT_19_PENALTY=0.575355
PRIMER_RIGHT_19_PENALTY=0.923750
PRIMER_LEFT_19_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_19_SEQUENCE=GTTGGTGGTGCGTTTAAGGA
PRIMER_LEFT_19=721,20
PRIMER_RIGHT_19=1699,20
PRIMER_LEFT_19_TM=59.425
PRIMER_RIGHT_19_TM=60.924
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=979
PRIMER_PAIR_20_PENALTY=1.601299
PRIMER_LEFT_20_PENALTY=0.519710
PRIMER_RIGHT_20_PENALTY=1.081589
PRIMER_LEFT_20_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_20_SEQUENCE=GGGTTGATGAAGAGACATGGA
PRIMER_LEFT_20=720,20
PRIMER_RIGHT_20=1668,21
PRIMER_LEFT_20_TM=60.520
PRIMER_RIGHT_20_TM=59.918
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=949
PRIMER_PAIR_21_PENALTY=1.656944
PRIMER_LEFT_21_PENALTY=0.575355
PRIMER_RIGHT_21_PENALTY=1.081589
PRIMER_LEFT_21_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_21_SEQUENCE=GGGTTGATGAAGAGACATGGA
PRIMER_LEFT_21=721,20
PRIMER_RIGHT_21=1668,21
PRIMER_LEFT_21_TM=59.425
PRIMER_RIGHT_21_TM=59.918
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=948
PRIMER_PAIR_22_PENALTY=1.723611
PRIMER_LEFT_22_PENALTY=0.519710
PRIMER_RIGHT_22_PENALTY=1.203901
PRIMER_LEFT_22_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_22_SEQUENCE=TTCGAGAGGTCGTTTGTTGG
PRIMER_LEFT_22=720,20
PRIMER_RIGHT_22=1714,20
PRIMER_LEFT_22_TM=60.520
PRIMER_RIGHT_22_TM=61.204
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=995
PRIMER_PAIR_23_PENALTY=1.723611
PRIMER_LEFT_23_PENALTY=0.519710
PRIMER_RIGHT_23_PENALTY=1.203901
PRIMER_LEFT_23_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_23_SEQUENCE=TTGGTTCGAGAGGTCGTTTG
PRIMER_LEFT_23=720,20
PRIMER_RIGHT_23=1718,20
PRIMER_LEFT_23_TM=60.520
PRIMER_RIGHT_23_TM=61.204
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=999
PRIMER_PAIR_24_PENALTY=1.741634
PRIMER_LEFT_24_PENALTY=1.215987
PRIMER_RIGHT_24_PENALTY=0.525647
PRIMER_LEFT_24_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_24_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_24=717,20
PRIMER_RIGHT_24=1710,20
PRIMER_LEFT_24_TM=61.216
PRIMER_RIGHT_24_TM=59.474
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.3000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=994
PRIMER_PAIR_25_PENALTY=1.761118
PRIMER_LEFT_25_PENALTY=1.215987
PRIMER_RIGHT_25_PENALTY=0.545130
PRIMER_LEFT_25_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_25_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_25=717,20
PRIMER_RIGHT_25=1711,20
PRIMER_LEFT_25_TM=61.216
PRIMER_RIGHT_25_TM=60.545
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=995
PRIMER_PAIR_26_PENALTY=1.766041
PRIMER_LEFT_26_PENALTY=1.240394
PRIMER_RIGHT_26_PENALTY=0.525647
PRIMER_LEFT_26_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_26_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_26=718,21
PRIMER_RIGHT_26=1710,20
PRIMER_LEFT_26_TM=59.760
PRIMER_RIGHT_26_TM=59.474
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=993
PRIMER_PAIR_27_PENALTY=1.779256
PRIMER_LEFT_27_PENALTY=0.575355
PRIMER_RIGHT_27_PENALTY=1.203901
PRIMER_LEFT_27_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_27_SEQUENCE=TTCGAGAGGTCGTTTGTTGG
PRIMER_LEFT_27=721,20
PRIMER_RIGHT_27=1714,20
PRIMER_LEFT_27_TM=59.425
PRIMER_RIGHT_27_TM=61.204
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=994
PRIMER_PAIR_28_PENALTY=1.779256
PRIMER_LEFT_28_PENALTY=0.575355
PRIMER_RIGHT_28_PENALTY=1.203901
PRIMER_LEFT_28_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_28_SEQUENCE=TTGGTTCGAGAGGTCGTTTG
PRIMER_LEFT_28=721,20
PRIMER_RIGHT_28=1718,20
PRIMER_LEFT_28_TM=59.425
PRIMER_RIGHT_28_TM=61.204
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=998
PRIMER_PAIR_29_PENALTY=1.779359
PRIMER_LEFT_29_PENALTY=1.253711
PRIMER_RIGHT_29_PENALTY=0.525647
PRIMER_LEFT_29_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_29_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_29=719,20
PRIMER_RIGHT_29=1710,20
PRIMER_LEFT_29_TM=58.746
PRIMER_RIGHT_29_TM=59.474
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=7.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=992
PRIMER_PAIR_30_PENALTY=1.785525
PRIMER_LEFT_30_PENALTY=1.240394
PRIMER_RIGHT_30_PENALTY=0.545130
PRIMER_LEFT_30_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_30_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_30=718,21
PRIMER_RIGHT_30=1711,20
PRIMER_LEFT_30_TM=59.760
PRIMER_RIGHT_30_TM=60.545
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=994
PRIMER_PAIR_31_PENALTY=1.798842
PRIMER_LEFT_31_PENALTY=1.253711
PRIMER_RIGHT_31_PENALTY=0.545130
PRIMER_LEFT_31_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_31_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_31=719,20
PRIMER_RIGHT_31=1711,20
PRIMER_LEFT_31_TM=58.746
PRIMER_RIGHT_31_TM=60.545
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=993
PRIMER_PAIR_32_PENALTY=1.873862
PRIMER_LEFT_32_PENALTY=0.519710
PRIMER_RIGHT_32_PENALTY=1.354152
PRIMER_LEFT_32_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_32_SEQUENCE=ATTGGTTCGAGAGGTCGTTT
PRIMER_LEFT_32=720,20
PRIMER_RIGHT_32=1719,20
PRIMER_LEFT_32_TM=60.520
PRIMER_RIGHT_32_TM=58.646
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=8.7000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=1000
PRIMER_PAIR_33_PENALTY=1.905668
PRIMER_LEFT_33_PENALTY=1.215987
PRIMER_RIGHT_33_PENALTY=0.689681
PRIMER_LEFT_33_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_33_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_33=717,20
PRIMER_RIGHT_33=1713,20
PRIMER_LEFT_33_TM=61.216
PRIMER_RIGHT_33_TM=60.690
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=997
PRIMER_PAIR_34_PENALTY=1.929508
PRIMER_LEFT_34_PENALTY=0.575355
PRIMER_RIGHT_34_PENALTY=1.354152
PRIMER_LEFT_34_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_34_SEQUENCE=ATTGGTTCGAGAGGTCGTTT
PRIMER_LEFT_34=721,20
PRIMER_RIGHT_34=1719,20
PRIMER_LEFT_34_TM=59.425
PRIMER_RIGHT_34_TM=58.646
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=8.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=999
PRIMER_PAIR_35_PENALTY=1.929650
PRIMER_LEFT_35_PENALTY=0.519710
PRIMER_RIGHT_35_PENALTY=1.409940
PRIMER_LEFT_35_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_35_SEQUENCE=GAGGTCGTTTGTTGGTGGTG
PRIMER_LEFT_35=720,20
PRIMER_RIGHT_35=1709,20
PRIMER_LEFT_35_TM=60.520
PRIMER_RIGHT_35_TM=61.410
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=990
PRIMER_PAIR_36_PENALTY=1.930075
PRIMER_LEFT_36_PENALTY=1.240394
PRIMER_RIGHT_36_PENALTY=0.689681
PRIMER_LEFT_36_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_36_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_36=718,21
PRIMER_RIGHT_36=1713,20
PRIMER_LEFT_36_TM=59.760
PRIMER_RIGHT_36_TM=60.690
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=996
PRIMER_PAIR_37_PENALTY=1.930075
PRIMER_LEFT_37_PENALTY=1.240394
PRIMER_RIGHT_37_PENALTY=0.689681
PRIMER_LEFT_37_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_37_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_37=718,21
PRIMER_RIGHT_37=1717,20
PRIMER_LEFT_37_TM=59.760
PRIMER_RIGHT_37_TM=60.690
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=1000
PRIMER_PAIR_38_PENALTY=1.943393
PRIMER_LEFT_38_PENALTY=1.253711
PRIMER_RIGHT_38_PENALTY=0.689681
PRIMER_LEFT_38_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_38_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_38=719,20
PRIMER_RIGHT_38=1713,20
PRIMER_LEFT_38_TM=58.746
PRIMER_RIGHT_38_TM=60.690
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=995
PRIMER_PAIR_39_PENALTY=1.943393
PRIMER_LEFT_39_PENALTY=1.253711
PRIMER_RIGHT_39_PENALTY=0.689681
PRIMER_LEFT_39_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_39_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_39=719,20
PRIMER_RIGHT_39=1717,20
PRIMER_LEFT_39_TM=58.746
PRIMER_RIGHT_39_TM=60.690
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=999
PRIMER_PAIR_40_PENALTY=1.984107
PRIMER_LEFT_40_PENALTY=0.902519
PRIMER_RIGHT_40_PENALTY=1.081589
PRIMER_LEFT_40_SEQUENCE=AAGGTGCCAAGTTAAGGGTG
PRIMER_RIGHT_40_SEQUENCE=GGGTTGATGAAGAGACATGGA
PRIMER_LEFT_40=669,20
PRIMER_RIGHT_40=1668,21
PRIMER_LEFT_40_TM=59.097
PRIMER_RIGHT_40_TM=59.918
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=1000
PRIMER_PAIR_41_PENALTY=1.985296
PRIMER_LEFT_41_PENALTY=0.575355
PRIMER_RIGHT_41_PENALTY=1.409940
PRIMER_LEFT_41_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_41_SEQUENCE=GAGGTCGTTTGTTGGTGGTG
PRIMER_LEFT_41=721,20
PRIMER_RIGHT_41=1709,20
PRIMER_LEFT_41_TM=59.425
PRIMER_RIGHT_41_TM=61.410
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=989
PRIMER_PAIR_42_PENALTY=2.029008
PRIMER_LEFT_42_PENALTY=1.215987
PRIMER_RIGHT_42_PENALTY=0.813021
PRIMER_LEFT_42_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_42_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_42=717,20
PRIMER_RIGHT_42=1716,20
PRIMER_LEFT_42_TM=61.216
PRIMER_RIGHT_42_TM=59.187
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=1000
PRIMER_PAIR_43_PENALTY=2.053415
PRIMER_LEFT_43_PENALTY=1.240394
PRIMER_RIGHT_43_PENALTY=0.813021
PRIMER_LEFT_43_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_43_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_43=718,21
PRIMER_RIGHT_43=1716,20
PRIMER_LEFT_43_TM=59.760
PRIMER_RIGHT_43_TM=59.187
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=999
PRIMER_PAIR_44_PENALTY=2.065669
PRIMER_LEFT_44_PENALTY=0.575355
PRIMER_RIGHT_44_PENALTY=1.490313
PRIMER_LEFT_44_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_44_SEQUENCE=GATTGGTTCGAGAGGTCGTTT
PRIMER_LEFT_44=721,20
PRIMER_RIGHT_44=1720,21
PRIMER_LEFT_44_TM=59.425
PRIMER_RIGHT_44_TM=60.490
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.7000
PRIMER_RIGHT_44_END_STABILITY=8.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=1000
PRIMER_PAIR_45_PENALTY=2.066732
PRIMER_LEFT_45_PENALTY=1.253711
PRIMER_RIGHT_45_PENALTY=0.813021
PRIMER_LEFT_45_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_45_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_45=719,20
PRIMER_RIGHT_45=1716,20
PRIMER_LEFT_45_TM=58.746
PRIMER_RIGHT_45_TM=59.187
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=998
PRIMER_PAIR_46_PENALTY=2.072081
PRIMER_LEFT_46_PENALTY=1.215987
PRIMER_RIGHT_46_PENALTY=0.856094
PRIMER_LEFT_46_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_46_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_46=717,20
PRIMER_RIGHT_46=1694,20
PRIMER_LEFT_46_TM=61.216
PRIMER_RIGHT_46_TM=60.856
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=978
PRIMER_PAIR_47_PENALTY=2.096488
PRIMER_LEFT_47_PENALTY=1.240394
PRIMER_RIGHT_47_PENALTY=0.856094
PRIMER_LEFT_47_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_47_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_47=718,21
PRIMER_RIGHT_47=1694,20
PRIMER_LEFT_47_TM=59.760
PRIMER_RIGHT_47_TM=60.856
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=977
PRIMER_PAIR_48_PENALTY=2.109805
PRIMER_LEFT_48_PENALTY=1.253711
PRIMER_RIGHT_48_PENALTY=0.856094
PRIMER_LEFT_48_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_48_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_48=719,20
PRIMER_RIGHT_48=1694,20
PRIMER_LEFT_48_TM=58.746
PRIMER_RIGHT_48_TM=60.856
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=976
PRIMER_PAIR_49_PENALTY=2.139737
PRIMER_LEFT_49_PENALTY=1.215987
PRIMER_RIGHT_49_PENALTY=0.923750
PRIMER_LEFT_49_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_49_SEQUENCE=GTTGGTGGTGCGTTTAAGGA
PRIMER_LEFT_49=717,20
PRIMER_RIGHT_49=1699,20
PRIMER_LEFT_49_TM=61.216
PRIMER_RIGHT_49_TM=60.924
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=983
=
SEQUENCE_ID=MAGIC.105_4_16674133_16674834_ws-0_no-0_28244_22449_TRUE
SEQUENCE_TARGET=950,801
SEQUENCE_TEMPLATE=GTGAACAAATTAGGGTTTTTGAAGTTTGAAGAAGGAGAGAAAGATGAGTGCTTCAAGGTTTATNAAGTGTGTCACTGTCGGCGACGGTGCTGTTGGAAAGACTTGTCTTCTCATCTCCTACACTAGCAACACTTTCCCCACGGTTAGCTTTAAAGTTCTCTTCTTTTACTTTGATTTCGTNAAATTTTAGCTTGGTGAAGTTGGTTTTGTTGTTGAAGCTTATGAGTTGGATTTAGTCACAAGTATTTGTTAGGTTTTCTTAAAGTCTTCTTCTTTCTGCTACCTTAGTGAATGTACTGATGTGATCTTTAGGATTTGAGAGACTCAGAGATTCATGTACCGTAGCAATGTTGTTGATCTGTTTAATGATCTTAGAAAGAGTGTTAGGTAACTTTCTGTAAGGAGATTTTGTAGCTGATGTTTTAGTNGAATTTTCTATGTGACAGGATTATGTGCCAACTGTGTTCGATAATTTCAGTGCCAATGTGATTGTTGATGGCAACACTATCAACTTGGGATTGTGGGATACTGCAGGCAAGTTGTTACCTTGGATTTTTTTGTCTTTTGAATTTCCTCCTCTTCCAGAGAGTCATTTGTGTTTTTGACTTGGTGAAACAGGGCAAGAGGACTACAATAGACTAAGACCTTTGAGCTATCGCGGTGCAGATGTCTTCTTACTTGCATTCTCACTTGTCAGCAAAGCTAGCTATGAAAATGTTTCTAAAAAGGTTTGATCTGTGGTCTTCTCCTGAAACTTCTTTCCTGTTTTATTCAGTTTACATTCAGTGATTCTGATAGAATACATTTGGTATCTTGCTTTTGATTCTTAGTGGGTTCCTGAACTGAGACATTATGCTCCTGGTGTTCCCATCATCCTCGTTGGAACAAAGCTTGGTTGGTTTTTTCTTAGCTCAACTCTTAATCGCCTGATTATCGGGGGTTTTCTGTTTTGGGGAACTCTTCTGATATTTTGATGACTGAGTCTCNTACAGATCTTCGANATGATAAGCAATTCTTTGCCGAGCACCCTGGTGCTGTGCCTATCTCTACCGCTCAGGTACCTTTCTCATTATCTTTCTAAACTGAATCCTAGGTGTTAATATTCTGCATGTTTCATATTTTGATGGTATCAAAACAGGGTGAAGAACTAAAGAAGCTGATTGGGGCGCCTGCTTATATCGAATGCAGTGCAAAAACTCAACAGGTATTAACCTGAGAGTCAATATCTTTATCTAATCTTTACCTGTCTAAAACCGCCAATNGAATCTGCTGATGATTCCTATATCTGTTATCTGTTCAACAGAATGTGAAAGCAGTGTTTGATGCGGCTATCAAGGTCGTTCTCCAGCCACCAAAAAACAAGAAGAAGAAGAAGAGAAAATCTCAGAAAGGTTGTTCTATACTCTGATTCAACAAGAAGAAAGGAATCAACAGGAGAATTAGAGCTATNAGTATTTGTTTTCTCCTTTTTCATAACTTGTTTACCCAAGGAATCAAAAATCTGTTGTTTGTAGTCTGAAAATGACAAAAGTTGAAGAACTAGTNACAAGTATTTGTATTCTTGTTTGCATTTTGATCTGTAAATATACCACGAATCCTTGATTTCTCTGTTTCCTAGATTCACACCAATTGTCTTAAGGATTATGTTCCAAACCTAAAGACAATGAGAGCTTACACGCGCGAACCAATGAGAATGAGAGANCACTTGTCTTTCTTGGACAANAGTATCTAAACCAGCTTATTTGAAACCTTCTGAATGAGCCAGAGTGTCTTCCATACATATCAATTCCACATAGACCTAACCTGATCTTTAGACTATAATCTTCAATGATTTCAGAACCTCAAAACTTTTGATTTTACATTTTTAATCAAATTTATTTGATATTCATTTTGCTTATTNACTAGACGTTTTCCCCTTGGAGATAATAGAATGATTGATTTTAACTCTCACCGGAAAAGACAAAAAAAAGGAAAAATAAATCATTGATTAAATAAAATTTATGGTGNANAAGCTTGGCTTCTCTTTACCAAACATATATGTTTGTTGATCAATCTCTTTCTTTGCGTTATCTGCCCCTTGATTTAGACCCACGTATTATTCGATTCCTAAACTAATTGAAATGATTAGATATGTGTCATGACGTTTAGTGAACTAATATTCACCTAATATGTCTTTCAATCACTTAAGAATTTGCATTATTCTTGTGAAAAACATAAATTCACATGCTGCTAGTAAAATATGTTAACAAATCAATTAAACATCAGAAAATAATANNNNNNNNCTTCATAAAGAAANTGGANGTTTTTTTAAATATATGAAGAAGAAAAGTGCGTAGTGTGCACGGGACTTAGTTCATTTAATTCTCGAGCCATTGCCGTACGGAGGAGGCTGCCTTCAAGGCTTAAGTCGAGGCGTGTTCGTTACGTTTAACCCGCAATAGCCGGTGGCTACACACTCACAAATCCCCTTTGACTCACCGGATATCNACGGTTTCCCGCTATTTTTTCCCCTAACTAACTTTTTTTCCTTTTCTTGTAAAACTCTTAAAAACAAAACTCGGTGTTGTCTCTTTGTTTGCTNACTCCTTTTCTTTCTCTCTCTCGCCGACACTTTCATTCTCACTTGTGTTCGTACGTAATGTTCTTGATCCGGTAGGTTTTTATTTGATTATAAACTCATTTCATTTAACAAATTGAGTCC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.355318
PRIMER_LEFT_0_PENALTY=0.214364
PRIMER_RIGHT_0_PENALTY=1.140954
PRIMER_LEFT_0_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_0_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_0=861,20
PRIMER_RIGHT_0=1775,21
PRIMER_LEFT_0_TM=59.786
PRIMER_RIGHT_0_TM=59.859
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=915
PRIMER_PAIR_1_PENALTY=1.385006
PRIMER_LEFT_1_PENALTY=0.244052
PRIMER_RIGHT_1_PENALTY=1.140954
PRIMER_LEFT_1_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_1_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_1=927,20
PRIMER_RIGHT_1=1775,21
PRIMER_LEFT_1_TM=59.756
PRIMER_RIGHT_1_TM=59.859
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=849
PRIMER_PAIR_2_PENALTY=1.466911
PRIMER_LEFT_2_PENALTY=0.325957
PRIMER_RIGHT_2_PENALTY=1.140954
PRIMER_LEFT_2_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_2_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_2=858,20
PRIMER_RIGHT_2=1775,21
PRIMER_LEFT_2_TM=60.326
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=918
PRIMER_PAIR_3_PENALTY=1.466911
PRIMER_LEFT_3_PENALTY=0.325957
PRIMER_RIGHT_3_PENALTY=1.140954
PRIMER_LEFT_3_SEQUENCE=CTCCTGGTGTTCCCATCATC
PRIMER_RIGHT_3_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_3=855,20
PRIMER_RIGHT_3=1775,21
PRIMER_LEFT_3_TM=60.326
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=921
PRIMER_PAIR_4_PENALTY=2.331676
PRIMER_LEFT_4_PENALTY=0.214364
PRIMER_RIGHT_4_PENALTY=2.117312
PRIMER_LEFT_4_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_4_SEQUENCE=GGAAGACACTCTGGCTCATTC
PRIMER_LEFT_4=861,20
PRIMER_RIGHT_4=1774,21
PRIMER_LEFT_4_TM=59.786
PRIMER_RIGHT_4_TM=58.883
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=914
PRIMER_PAIR_5_PENALTY=2.353447
PRIMER_LEFT_5_PENALTY=0.214364
PRIMER_RIGHT_5_PENALTY=2.139083
PRIMER_LEFT_5_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_5_SEQUENCE=ATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_5=861,20
PRIMER_RIGHT_5=1776,22
PRIMER_LEFT_5_TM=59.786
PRIMER_RIGHT_5_TM=60.139
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=916
PRIMER_PAIR_6_PENALTY=2.361364
PRIMER_LEFT_6_PENALTY=0.244052
PRIMER_RIGHT_6_PENALTY=2.117312
PRIMER_LEFT_6_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_6_SEQUENCE=GGAAGACACTCTGGCTCATTC
PRIMER_LEFT_6=927,20
PRIMER_RIGHT_6=1774,21
PRIMER_LEFT_6_TM=59.756
PRIMER_RIGHT_6_TM=58.883
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=848
PRIMER_PAIR_7_PENALTY=2.383135
PRIMER_LEFT_7_PENALTY=0.244052
PRIMER_RIGHT_7_PENALTY=2.139083
PRIMER_LEFT_7_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_7_SEQUENCE=ATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_7=927,20
PRIMER_RIGHT_7=1776,22
PRIMER_LEFT_7_TM=59.756
PRIMER_RIGHT_7_TM=60.139
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=850
PRIMER_PAIR_8_PENALTY=2.443269
PRIMER_LEFT_8_PENALTY=0.325957
PRIMER_RIGHT_8_PENALTY=2.117312
PRIMER_LEFT_8_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_8_SEQUENCE=GGAAGACACTCTGGCTCATTC
PRIMER_LEFT_8=858,20
PRIMER_RIGHT_8=1774,21
PRIMER_LEFT_8_TM=60.326
PRIMER_RIGHT_8_TM=58.883
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=917
PRIMER_PAIR_9_PENALTY=2.443269
PRIMER_LEFT_9_PENALTY=0.325957
PRIMER_RIGHT_9_PENALTY=2.117312
PRIMER_LEFT_9_SEQUENCE=CTCCTGGTGTTCCCATCATC
PRIMER_RIGHT_9_SEQUENCE=GGAAGACACTCTGGCTCATTC
PRIMER_LEFT_9=855,20
PRIMER_RIGHT_9=1774,21
PRIMER_LEFT_9_TM=60.326
PRIMER_RIGHT_9_TM=58.883
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=920
PRIMER_PAIR_10_PENALTY=2.465040
PRIMER_LEFT_10_PENALTY=0.325957
PRIMER_RIGHT_10_PENALTY=2.139083
PRIMER_LEFT_10_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_10_SEQUENCE=ATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_10=858,20
PRIMER_RIGHT_10=1776,22
PRIMER_LEFT_10_TM=60.326
PRIMER_RIGHT_10_TM=60.139
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.455
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=919
PRIMER_PAIR_11_PENALTY=2.465040
PRIMER_LEFT_11_PENALTY=0.325957
PRIMER_RIGHT_11_PENALTY=2.139083
PRIMER_LEFT_11_SEQUENCE=CTCCTGGTGTTCCCATCATC
PRIMER_RIGHT_11_SEQUENCE=ATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_11=855,20
PRIMER_RIGHT_11=1776,22
PRIMER_LEFT_11_TM=60.326
PRIMER_RIGHT_11_TM=60.139
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=922
PRIMER_PAIR_12_PENALTY=2.688814
PRIMER_LEFT_12_PENALTY=1.547860
PRIMER_RIGHT_12_PENALTY=1.140954
PRIMER_LEFT_12_SEQUENCE=TGATTATCGGGGGTTTTCTGT
PRIMER_RIGHT_12_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_12=927,21
PRIMER_RIGHT_12=1775,21
PRIMER_LEFT_12_TM=60.548
PRIMER_RIGHT_12_TM=59.859
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=849
PRIMER_PAIR_13_PENALTY=2.723833
PRIMER_LEFT_13_PENALTY=1.582879
PRIMER_RIGHT_13_PENALTY=1.140954
PRIMER_LEFT_13_SEQUENCE=TTATCGGGGGTTTTCTGTTT
PRIMER_RIGHT_13_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_13=930,20
PRIMER_RIGHT_13=1775,21
PRIMER_LEFT_13_TM=58.417
PRIMER_RIGHT_13_TM=59.859
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=846
PRIMER_PAIR_14_PENALTY=2.809715
PRIMER_LEFT_14_PENALTY=1.668761
PRIMER_RIGHT_14_PENALTY=1.140954
PRIMER_LEFT_14_SEQUENCE=CTGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_14_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_14=926,21
PRIMER_RIGHT_14=1775,21
PRIMER_LEFT_14_TM=60.669
PRIMER_RIGHT_14_TM=59.859
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=850
PRIMER_PAIR_15_PENALTY=2.857176
PRIMER_LEFT_15_PENALTY=0.214364
PRIMER_RIGHT_15_PENALTY=2.642812
PRIMER_LEFT_15_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_15_SEQUENCE=TGTATGGAAGACACTCTGGCTC
PRIMER_LEFT_15=861,20
PRIMER_RIGHT_15=1779,22
PRIMER_LEFT_15_TM=59.786
PRIMER_RIGHT_15_TM=59.357
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=919
PRIMER_PAIR_16_PENALTY=2.886864
PRIMER_LEFT_16_PENALTY=0.244052
PRIMER_RIGHT_16_PENALTY=2.642812
PRIMER_LEFT_16_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_16_SEQUENCE=TGTATGGAAGACACTCTGGCTC
PRIMER_LEFT_16=927,20
PRIMER_RIGHT_16=1779,22
PRIMER_LEFT_16_TM=59.756
PRIMER_RIGHT_16_TM=59.357
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=853
PRIMER_PAIR_17_PENALTY=2.949383
PRIMER_LEFT_17_PENALTY=1.808429
PRIMER_RIGHT_17_PENALTY=1.140954
PRIMER_LEFT_17_SEQUENCE=GATTATCGGGGGTTTTCTGTT
PRIMER_RIGHT_17_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_17=928,21
PRIMER_RIGHT_17=1775,21
PRIMER_LEFT_17_TM=59.192
PRIMER_RIGHT_17_TM=59.859
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=848
PRIMER_PAIR_18_PENALTY=2.968769
PRIMER_LEFT_18_PENALTY=0.325957
PRIMER_RIGHT_18_PENALTY=2.642812
PRIMER_LEFT_18_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_18_SEQUENCE=TGTATGGAAGACACTCTGGCTC
PRIMER_LEFT_18=858,20
PRIMER_RIGHT_18=1779,22
PRIMER_LEFT_18_TM=60.326
PRIMER_RIGHT_18_TM=59.357
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=922
PRIMER_PAIR_19_PENALTY=2.968769
PRIMER_LEFT_19_PENALTY=0.325957
PRIMER_RIGHT_19_PENALTY=2.642812
PRIMER_LEFT_19_SEQUENCE=CTCCTGGTGTTCCCATCATC
PRIMER_RIGHT_19_SEQUENCE=TGTATGGAAGACACTCTGGCTC
PRIMER_LEFT_19=855,20
PRIMER_RIGHT_19=1779,22
PRIMER_LEFT_19_TM=60.326
PRIMER_RIGHT_19_TM=59.357
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=925
PRIMER_PAIR_20_PENALTY=3.017088
PRIMER_LEFT_20_PENALTY=1.876134
PRIMER_RIGHT_20_PENALTY=1.140954
PRIMER_LEFT_20_SEQUENCE=TGTTCCCATCATCCTCGTTG
PRIMER_RIGHT_20_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_20=862,20
PRIMER_RIGHT_20=1775,21
PRIMER_LEFT_20_TM=61.876
PRIMER_RIGHT_20_TM=59.859
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.7000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=914
PRIMER_PAIR_21_PENALTY=3.121692
PRIMER_LEFT_21_PENALTY=1.980738
PRIMER_RIGHT_21_PENALTY=1.140954
PRIMER_LEFT_21_SEQUENCE=CATTATGCTCCTGGTGTTCC
PRIMER_RIGHT_21_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_21=848,20
PRIMER_RIGHT_21=1775,21
PRIMER_LEFT_21_TM=58.019
PRIMER_RIGHT_21_TM=59.859
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=928
PRIMER_PAIR_22_PENALTY=3.147769
PRIMER_LEFT_22_PENALTY=2.006815
PRIMER_RIGHT_22_PENALTY=1.140954
PRIMER_LEFT_22_SEQUENCE=CTGATTATCGGGGGTTTTCT
PRIMER_RIGHT_22_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_22=926,20
PRIMER_RIGHT_22=1775,21
PRIMER_LEFT_22_TM=57.993
PRIMER_RIGHT_22_TM=59.859
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=850
PRIMER_PAIR_23_PENALTY=3.200187
PRIMER_LEFT_23_PENALTY=0.214364
PRIMER_RIGHT_23_PENALTY=2.985824
PRIMER_LEFT_23_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_23_SEQUENCE=GAAGACACTCTGGCTCATTCA
PRIMER_LEFT_23=861,20
PRIMER_RIGHT_23=1773,21
PRIMER_LEFT_23_TM=59.786
PRIMER_RIGHT_23_TM=58.014
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=913
PRIMER_PAIR_24_PENALTY=3.215510
PRIMER_LEFT_24_PENALTY=2.074556
PRIMER_RIGHT_24_PENALTY=1.140954
PRIMER_LEFT_24_SEQUENCE=ACATTATGCTCCTGGTGTTCC
PRIMER_RIGHT_24_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_24=847,21
PRIMER_RIGHT_24=1775,21
PRIMER_LEFT_24_TM=58.925
PRIMER_RIGHT_24_TM=59.859
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=929
PRIMER_PAIR_25_PENALTY=3.229875
PRIMER_LEFT_25_PENALTY=0.244052
PRIMER_RIGHT_25_PENALTY=2.985824
PRIMER_LEFT_25_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_25_SEQUENCE=GAAGACACTCTGGCTCATTCA
PRIMER_LEFT_25=927,20
PRIMER_RIGHT_25=1773,21
PRIMER_LEFT_25_TM=59.756
PRIMER_RIGHT_25_TM=58.014
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=847
PRIMER_PAIR_26_PENALTY=3.248908
PRIMER_LEFT_26_PENALTY=2.107954
PRIMER_RIGHT_26_PENALTY=1.140954
PRIMER_LEFT_26_SEQUENCE=CCTGGTGTTCCCATCATCCT
PRIMER_RIGHT_26_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_26=857,20
PRIMER_RIGHT_26=1775,21
PRIMER_LEFT_26_TM=62.108
PRIMER_RIGHT_26_TM=59.859
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.8000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=919
PRIMER_PAIR_27_PENALTY=3.253477
PRIMER_LEFT_27_PENALTY=1.136165
PRIMER_RIGHT_27_PENALTY=2.117312
PRIMER_LEFT_27_SEQUENCE=ATTATGCTCCTGGTGTTCCC
PRIMER_RIGHT_27_SEQUENCE=GGAAGACACTCTGGCTCATTC
PRIMER_LEFT_27=849,20
PRIMER_RIGHT_27=1774,21
PRIMER_LEFT_27_TM=58.864
PRIMER_RIGHT_27_TM=58.883
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=926
PRIMER_PAIR_28_PENALTY=3.264812
PRIMER_LEFT_28_PENALTY=2.123858
PRIMER_RIGHT_28_PENALTY=1.140954
PRIMER_LEFT_28_SEQUENCE=GGTGTTCCCATCATCCTCGT
PRIMER_RIGHT_28_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_28=860,20
PRIMER_RIGHT_28=1775,21
PRIMER_LEFT_28_TM=62.124
PRIMER_RIGHT_28_TM=59.859
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.1000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=916
PRIMER_PAIR_29_PENALTY=3.292910
PRIMER_LEFT_29_PENALTY=2.151956
PRIMER_RIGHT_29_PENALTY=1.140954
PRIMER_LEFT_29_SEQUENCE=GATTATCGGGGGTTTTCTGT
PRIMER_RIGHT_29_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_29=928,20
PRIMER_RIGHT_29=1775,21
PRIMER_LEFT_29_TM=57.848
PRIMER_RIGHT_29_TM=59.859
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=848
PRIMER_PAIR_30_PENALTY=3.311780
PRIMER_LEFT_30_PENALTY=0.325957
PRIMER_RIGHT_30_PENALTY=2.985824
PRIMER_LEFT_30_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_30_SEQUENCE=GAAGACACTCTGGCTCATTCA
PRIMER_LEFT_30=858,20
PRIMER_RIGHT_30=1773,21
PRIMER_LEFT_30_TM=60.326
PRIMER_RIGHT_30_TM=58.014
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=916
PRIMER_PAIR_31_PENALTY=3.311780
PRIMER_LEFT_31_PENALTY=0.325957
PRIMER_RIGHT_31_PENALTY=2.985824
PRIMER_LEFT_31_SEQUENCE=CTCCTGGTGTTCCCATCATC
PRIMER_RIGHT_31_SEQUENCE=GAAGACACTCTGGCTCATTCA
PRIMER_LEFT_31=855,20
PRIMER_RIGHT_31=1773,21
PRIMER_LEFT_31_TM=60.326
PRIMER_RIGHT_31_TM=58.014
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=919
PRIMER_PAIR_32_PENALTY=3.349955
PRIMER_LEFT_32_PENALTY=0.214364
PRIMER_RIGHT_32_PENALTY=3.135591
PRIMER_LEFT_32_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_32_SEQUENCE=GGAAGACACTCTGGCTCATT
PRIMER_LEFT_32=861,20
PRIMER_RIGHT_32=1774,20
PRIMER_LEFT_32_TM=59.786
PRIMER_RIGHT_32_TM=56.864
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=914
PRIMER_PAIR_33_PENALTY=3.358682
PRIMER_LEFT_33_PENALTY=0.214364
PRIMER_RIGHT_33_PENALTY=3.144318
PRIMER_LEFT_33_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_33_SEQUENCE=TATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_33=861,20
PRIMER_RIGHT_33=1777,23
PRIMER_LEFT_33_TM=59.786
PRIMER_RIGHT_33_TM=60.144
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=43.478
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=917
PRIMER_PAIR_34_PENALTY=3.379643
PRIMER_LEFT_34_PENALTY=0.244052
PRIMER_RIGHT_34_PENALTY=3.135591
PRIMER_LEFT_34_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_34_SEQUENCE=GGAAGACACTCTGGCTCATT
PRIMER_LEFT_34=927,20
PRIMER_RIGHT_34=1774,20
PRIMER_LEFT_34_TM=59.756
PRIMER_RIGHT_34_TM=56.864
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=848
PRIMER_PAIR_35_PENALTY=3.384827
PRIMER_LEFT_35_PENALTY=2.243873
PRIMER_RIGHT_35_PENALTY=1.140954
PRIMER_LEFT_35_SEQUENCE=AGCTCAACTCTTAATCGCCTG
PRIMER_RIGHT_35_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_35=908,21
PRIMER_RIGHT_35=1775,21
PRIMER_LEFT_35_TM=58.756
PRIMER_RIGHT_35_TM=59.859
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=868
PRIMER_PAIR_36_PENALTY=3.388370
PRIMER_LEFT_36_PENALTY=0.244052
PRIMER_RIGHT_36_PENALTY=3.144318
PRIMER_LEFT_36_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_36_SEQUENCE=TATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_36=927,20
PRIMER_RIGHT_36=1777,23
PRIMER_LEFT_36_TM=59.756
PRIMER_RIGHT_36_TM=60.144
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=851
PRIMER_PAIR_37_PENALTY=3.414237
PRIMER_LEFT_37_PENALTY=2.273283
PRIMER_RIGHT_37_PENALTY=1.140954
PRIMER_LEFT_37_SEQUENCE=GCTCAACTCTTAATCGCCTG
PRIMER_RIGHT_37_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_37=909,20
PRIMER_RIGHT_37=1775,21
PRIMER_LEFT_37_TM=57.727
PRIMER_RIGHT_37_TM=59.859
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=867
PRIMER_PAIR_38_PENALTY=3.461548
PRIMER_LEFT_38_PENALTY=0.325957
PRIMER_RIGHT_38_PENALTY=3.135591
PRIMER_LEFT_38_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_38_SEQUENCE=GGAAGACACTCTGGCTCATT
PRIMER_LEFT_38=858,20
PRIMER_RIGHT_38=1774,20
PRIMER_LEFT_38_TM=60.326
PRIMER_RIGHT_38_TM=56.864
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=917
PRIMER_PAIR_39_PENALTY=3.461548
PRIMER_LEFT_39_PENALTY=0.325957
PRIMER_RIGHT_39_PENALTY=3.135591
PRIMER_LEFT_39_SEQUENCE=CTCCTGGTGTTCCCATCATC
PRIMER_RIGHT_39_SEQUENCE=GGAAGACACTCTGGCTCATT
PRIMER_LEFT_39=855,20
PRIMER_RIGHT_39=1774,20
PRIMER_LEFT_39_TM=60.326
PRIMER_RIGHT_39_TM=56.864
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=920
PRIMER_PAIR_40_PENALTY=3.470275
PRIMER_LEFT_40_PENALTY=0.325957
PRIMER_RIGHT_40_PENALTY=3.144318
PRIMER_LEFT_40_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_40_SEQUENCE=TATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_40=858,20
PRIMER_RIGHT_40=1777,23
PRIMER_LEFT_40_TM=60.326
PRIMER_RIGHT_40_TM=60.144
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=43.478
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=920
PRIMER_PAIR_41_PENALTY=3.470275
PRIMER_LEFT_41_PENALTY=0.325957
PRIMER_RIGHT_41_PENALTY=3.144318
PRIMER_LEFT_41_SEQUENCE=CTCCTGGTGTTCCCATCATC
PRIMER_RIGHT_41_SEQUENCE=TATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_41=855,20
PRIMER_RIGHT_41=1777,23
PRIMER_LEFT_41_TM=60.326
PRIMER_RIGHT_41_TM=60.144
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=43.478
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.6000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=923
PRIMER_PAIR_42_PENALTY=3.546727
PRIMER_LEFT_42_PENALTY=2.405773
PRIMER_RIGHT_42_PENALTY=1.140954
PRIMER_LEFT_42_SEQUENCE=GATTATCGGGGGTTTTCTGTTT
PRIMER_RIGHT_42_SEQUENCE=TGGAAGACACTCTGGCTCATT
PRIMER_LEFT_42=928,22
PRIMER_RIGHT_42=1775,21
PRIMER_LEFT_42_TM=60.406
PRIMER_RIGHT_42_TM=59.859
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=848
PRIMER_PAIR_43_PENALTY=3.557110
PRIMER_LEFT_43_PENALTY=0.214364
PRIMER_RIGHT_43_PENALTY=3.342747
PRIMER_LEFT_43_SEQUENCE=GTGTTCCCATCATCCTCGTT
PRIMER_RIGHT_43_SEQUENCE=ATGTATGGAAGACACTCTGGCTC
PRIMER_LEFT_43=861,20
PRIMER_RIGHT_43=1780,23
PRIMER_LEFT_43_TM=59.786
PRIMER_RIGHT_43_TM=59.657
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.826
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=7.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=920
PRIMER_PAIR_44_PENALTY=3.586798
PRIMER_LEFT_44_PENALTY=0.244052
PRIMER_RIGHT_44_PENALTY=3.342747
PRIMER_LEFT_44_SEQUENCE=TGATTATCGGGGGTTTTCTG
PRIMER_RIGHT_44_SEQUENCE=ATGTATGGAAGACACTCTGGCTC
PRIMER_LEFT_44=927,20
PRIMER_RIGHT_44=1780,23
PRIMER_LEFT_44_TM=59.756
PRIMER_RIGHT_44_TM=59.657
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=47.826
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=854
PRIMER_PAIR_45_PENALTY=3.665172
PRIMER_LEFT_45_PENALTY=1.547860
PRIMER_RIGHT_45_PENALTY=2.117312
PRIMER_LEFT_45_SEQUENCE=TGATTATCGGGGGTTTTCTGT
PRIMER_RIGHT_45_SEQUENCE=GGAAGACACTCTGGCTCATTC
PRIMER_LEFT_45=927,21
PRIMER_RIGHT_45=1774,21
PRIMER_LEFT_45_TM=60.548
PRIMER_RIGHT_45_TM=58.883
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=848
PRIMER_PAIR_46_PENALTY=3.668703
PRIMER_LEFT_46_PENALTY=0.325957
PRIMER_RIGHT_46_PENALTY=3.342747
PRIMER_LEFT_46_SEQUENCE=CTGGTGTTCCCATCATCCTC
PRIMER_RIGHT_46_SEQUENCE=ATGTATGGAAGACACTCTGGCTC
PRIMER_LEFT_46=858,20
PRIMER_RIGHT_46=1780,23
PRIMER_LEFT_46_TM=60.326
PRIMER_RIGHT_46_TM=59.657
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=47.826
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=923
PRIMER_PAIR_47_PENALTY=3.686943
PRIMER_LEFT_47_PENALTY=1.547860
PRIMER_RIGHT_47_PENALTY=2.139083
PRIMER_LEFT_47_SEQUENCE=TGATTATCGGGGGTTTTCTGT
PRIMER_RIGHT_47_SEQUENCE=ATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_47=927,21
PRIMER_RIGHT_47=1776,22
PRIMER_LEFT_47_TM=60.548
PRIMER_RIGHT_47_TM=60.139
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=850
PRIMER_PAIR_48_PENALTY=3.700192
PRIMER_LEFT_48_PENALTY=1.582879
PRIMER_RIGHT_48_PENALTY=2.117312
PRIMER_LEFT_48_SEQUENCE=TTATCGGGGGTTTTCTGTTT
PRIMER_RIGHT_48_SEQUENCE=GGAAGACACTCTGGCTCATTC
PRIMER_LEFT_48=930,20
PRIMER_RIGHT_48=1774,21
PRIMER_LEFT_48_TM=58.417
PRIMER_RIGHT_48_TM=58.883
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=845
PRIMER_PAIR_49_PENALTY=3.721963
PRIMER_LEFT_49_PENALTY=1.582879
PRIMER_RIGHT_49_PENALTY=2.139083
PRIMER_LEFT_49_SEQUENCE=TTATCGGGGGTTTTCTGTTT
PRIMER_RIGHT_49_SEQUENCE=ATGGAAGACACTCTGGCTCATT
PRIMER_LEFT_49=930,20
PRIMER_RIGHT_49=1776,22
PRIMER_LEFT_49_TM=58.417
PRIMER_RIGHT_49_TM=60.139
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=847
=
SEQUENCE_ID=MAGIC.105_4_17523538_17523758_can-0_rsch-4_6719_36672_TRUE
SEQUENCE_TARGET=950,320
SEQUENCE_TEMPLATE=AANAATAATAATACTATTAGCTAAACACCACTTCATTTCTGGTTGTCATCGTTGTGATAGTTGACAATTAACCACATATTGNCCATCATTCCAATTCCAAAATTTGACTATAACACATTATTCTGAACCACAGAGAACAGTTTATATTATTAATTTCACTTAGTTATATANAAANTANTATATCCGAAGAAGCAAGAACAAATAACGAGATGTCACCGGGCAAGAAGGGACTCAACGAGTTTAAGCCATCACCAATAGAAATTGTCGGATTCTTGTAGGACCCATNTAAAGNTACGTGGCGTCTCCTGGCTCGAAAATACGATGCGTGGCTGCTCCAGTTTNGNCCACGTNNTTCCCCTGCCACTCCCTTNTCTTGCTATTCCTACACTTNCGNTTTGTTCNGTNGNCTCTCAGCNCTAANGCCACGTCTATNGGTCACATATATTTATTCATATCATATTAGTTNATNACTGAATTTNNNNNNNNNNTGTATATTTGGACGTTGATTATAACGTAACCTAAGGACATGAGATTTTGCGTAGTNATAGTATTCATGATCNACCGGAAGAGTTAATACTGTTTCTCGCAAATATATTACTCACATTTAAACCGATCAGTGACATTCATCAAAACANTCAAATCATTTGAATACAACTACTTGACATTATTCCTTCTATCTTAATTACTAAGATATGCCAATTCTNTCCTCAGTAGATTNGGTAAATTGTCTTTACANAAGAAATTAGAAAGATAAAGTAGTAGTAGCTAGTCCTTACGCAACTTGGTAAACCAAAACCGGAATAAAATCTACTTGCAAANAAGAAGTAAAACCATGGTCGCCAACAAATATGCTTACTCTATTTAGCTTAAGTCATGAATCATGATCACTCNNTTTTATTCTTTATCACCAATNAAATTCATTGTANTTTAGGATGTATAATGTTGACATCAACGTAAGCAAACTCTGGCTTTTTTCCATGAGTCCGACTGGTATATTTTANTAAATGTGTGATTAACAAACCTTTTGCTTGTCTACTAACCAAANAAATAGTGGCAACAAGTAAATTAGATCAANTATAAACACNNTGAACAATATGAGCCGTTGATCATAAATTAGGCGTAAGAAATAGATACGTACAGTAGAAAANAAACATANATGTATATACACACCCAAAAANAAAGGAATAAATAACCAATGAATTAAAAGTACATTAAAGGATNCCGACAGCTTCTTGGCCAATCAATATCACAGATTTAAAAGTGACAAGAAAGTTCATAAATTTCCANTATACAAAAATGGGAACGAGTATTGCATTTAAAATTGTAACCTAGGCNTAAAGGGCAGCAATAGNTTTNNTTTTNTAAATCGGGGNATTCAATTTTAACTTAAATTCGTAAACCCACCACGCCAACCCCAAGAGCATTATATCAATTTATTCACTCATTAATTTATCTGTTCGTAAAACAAAATGNATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNGTAGAGTAGGCGAANTACATGTCTTGTNAATTANTATATANTTTNTTTTTGTCTTANTTNAACAANNACTATATGTTATATTCCTNAAATTTTGNATACNTTTATCATGCAAATCANNTTTTNNANNAAAACGTAAAAGAAAAAGANNGANAANGGTAACAACAAATTCGNNNNAAANAAATAAAAGGTGAGTCCCTGACATGGTAATTATAGCTAATAATAAAGGGTTAAAATATCTACGCAAACACAAAAGCTGTCTTCTTCCCCANCCACTTNTACAGCCTGTCATCACTCCTATTGGTTCNNAAAACGACAAANTCCTACGTGGCATCCANNNTTNNNCTTCTCTTCTTCTGCAAATTNTCTCTATTTATACACAAACCTTCCATTTCTCTTCCCATAATTTATTNATTTCACCGNAAAAACTGAGTCAACTCAGAGCGAGTTACCATTACCAAGAATCATTTAACACTACTGAATACATATGGGTATATGCAGCTCGAGCGAGTCGACTCAAGTCGCGACGGCGAAGCTGATNTTACAAGATGGNAGGATGATGGAGTTTNCGAATCCCGTTAAAGTCGGATACGTNTTGCTCAAGTATCCGATGTGTTTTATCTGCAACTCCGACGATATGGACTTNGACGACGCNGTTGCCGCTATTAGCGCTGACGAAGAGCTTCAGCTTGGTCAGAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.642524
PRIMER_LEFT_0_PENALTY=2.735382
PRIMER_RIGHT_0_PENALTY=1.907142
PRIMER_LEFT_0_SEQUENCE=AAGGACATGAGATTTTGCGT
PRIMER_RIGHT_0_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_0=520,20
PRIMER_RIGHT_0=1313,20
PRIMER_LEFT_0_TM=57.265
PRIMER_RIGHT_0_TM=58.093
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=794
PRIMER_PAIR_1_PENALTY=4.875060
PRIMER_LEFT_1_PENALTY=2.967917
PRIMER_RIGHT_1_PENALTY=1.907142
PRIMER_LEFT_1_SEQUENCE=AAATACGATGCGTGGCTGCT
PRIMER_RIGHT_1_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_1=314,20
PRIMER_RIGHT_1=1313,20
PRIMER_LEFT_1_TM=62.968
PRIMER_RIGHT_1_TM=58.093
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=1000
PRIMER_PAIR_2_PENALTY=5.034725
PRIMER_LEFT_2_PENALTY=2.735382
PRIMER_RIGHT_2_PENALTY=2.299343
PRIMER_LEFT_2_SEQUENCE=AAGGACATGAGATTTTGCGT
PRIMER_RIGHT_2_SEQUENCE=GATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_2=520,20
PRIMER_RIGHT_2=1429,22
PRIMER_LEFT_2_TM=57.265
PRIMER_RIGHT_2_TM=59.701
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=910
PRIMER_PAIR_3_PENALTY=5.156447
PRIMER_LEFT_3_PENALTY=2.735382
PRIMER_RIGHT_3_PENALTY=2.421065
PRIMER_LEFT_3_SEQUENCE=AAGGACATGAGATTTTGCGT
PRIMER_RIGHT_3_SEQUENCE=TATAATGCTCTTGGGGTTGG
PRIMER_LEFT_3=520,20
PRIMER_RIGHT_3=1427,20
PRIMER_LEFT_3_TM=57.265
PRIMER_RIGHT_3_TM=57.579
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=908
PRIMER_PAIR_4_PENALTY=5.457082
PRIMER_LEFT_4_PENALTY=3.549940
PRIMER_RIGHT_4_PENALTY=1.907142
PRIMER_LEFT_4_SEQUENCE=AATACGATGCGTGGCTGCTC
PRIMER_RIGHT_4_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_4=315,20
PRIMER_RIGHT_4=1313,20
PRIMER_LEFT_4_TM=63.550
PRIMER_RIGHT_4_TM=58.093
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=999
PRIMER_PAIR_5_PENALTY=5.504448
PRIMER_LEFT_5_PENALTY=3.597306
PRIMER_RIGHT_5_PENALTY=1.907142
PRIMER_LEFT_5_SEQUENCE=AAGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_5_SEQUENCE=AAATGCAATACTCGTTCCCA
PRIMER_LEFT_5=520,22
PRIMER_RIGHT_5=1316,20
PRIMER_LEFT_5_TM=58.403
PRIMER_RIGHT_5_TM=58.093
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.4000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=797
PRIMER_PAIR_6_PENALTY=5.769189
PRIMER_LEFT_6_PENALTY=2.735382
PRIMER_RIGHT_6_PENALTY=3.033807
PRIMER_LEFT_6_SEQUENCE=AAGGACATGAGATTTTGCGT
PRIMER_RIGHT_6_SEQUENCE=TGATATAATGCTCTTGGGGTTG
PRIMER_LEFT_6=520,20
PRIMER_RIGHT_6=1430,22
PRIMER_LEFT_6_TM=57.265
PRIMER_RIGHT_6_TM=58.966
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=911
PRIMER_PAIR_7_PENALTY=5.773636
PRIMER_LEFT_7_PENALTY=2.735382
PRIMER_RIGHT_7_PENALTY=3.038254
PRIMER_LEFT_7_SEQUENCE=AAGGACATGAGATTTTGCGT
PRIMER_RIGHT_7_SEQUENCE=ATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_7=520,20
PRIMER_RIGHT_7=1428,21
PRIMER_LEFT_7_TM=57.265
PRIMER_RIGHT_7_TM=57.962
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.9000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=909
PRIMER_PAIR_8_PENALTY=5.896649
PRIMER_LEFT_8_PENALTY=3.597306
PRIMER_RIGHT_8_PENALTY=2.299343
PRIMER_LEFT_8_SEQUENCE=AAGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_8_SEQUENCE=GATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_8=520,22
PRIMER_RIGHT_8=1429,22
PRIMER_LEFT_8_TM=58.403
PRIMER_RIGHT_8_TM=59.701
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=910
PRIMER_PAIR_9_PENALTY=5.920465
PRIMER_LEFT_9_PENALTY=4.013323
PRIMER_RIGHT_9_PENALTY=1.907142
PRIMER_LEFT_9_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_9_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_9=521,21
PRIMER_RIGHT_9=1313,20
PRIMER_LEFT_9_TM=56.987
PRIMER_RIGHT_9_TM=58.093
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.4000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=793
PRIMER_PAIR_10_PENALTY=5.920465
PRIMER_LEFT_10_PENALTY=4.013323
PRIMER_RIGHT_10_PENALTY=1.907142
PRIMER_LEFT_10_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_10_SEQUENCE=AAATGCAATACTCGTTCCCA
PRIMER_LEFT_10=521,21
PRIMER_RIGHT_10=1316,20
PRIMER_LEFT_10_TM=56.987
PRIMER_RIGHT_10_TM=58.093
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.4000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=796
PRIMER_PAIR_11_PENALTY=6.014851
PRIMER_LEFT_11_PENALTY=4.107708
PRIMER_RIGHT_11_PENALTY=1.907142
PRIMER_LEFT_11_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_11_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_11=521,22
PRIMER_RIGHT_11=1313,20
PRIMER_LEFT_11_TM=57.892
PRIMER_RIGHT_11_TM=58.093
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.1000
PRIMER_RIGHT_11_END_STABILITY=7.2000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=793
PRIMER_PAIR_12_PENALTY=6.014851
PRIMER_LEFT_12_PENALTY=4.107708
PRIMER_RIGHT_12_PENALTY=1.907142
PRIMER_LEFT_12_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_12_SEQUENCE=AAATGCAATACTCGTTCCCA
PRIMER_LEFT_12=521,22
PRIMER_RIGHT_12=1316,20
PRIMER_LEFT_12_TM=57.892
PRIMER_RIGHT_12_TM=58.093
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=5.1000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=796
PRIMER_PAIR_13_PENALTY=6.018371
PRIMER_LEFT_13_PENALTY=3.597306
PRIMER_RIGHT_13_PENALTY=2.421065
PRIMER_LEFT_13_SEQUENCE=AAGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_13_SEQUENCE=TATAATGCTCTTGGGGTTGG
PRIMER_LEFT_13=520,22
PRIMER_RIGHT_13=1427,20
PRIMER_LEFT_13_TM=58.403
PRIMER_RIGHT_13_TM=57.579
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=908
PRIMER_PAIR_14_PENALTY=6.020050
PRIMER_LEFT_14_PENALTY=2.735382
PRIMER_RIGHT_14_PENALTY=3.284668
PRIMER_LEFT_14_SEQUENCE=AAGGACATGAGATTTTGCGT
PRIMER_RIGHT_14_SEQUENCE=GCAATACTCGTTCCCATTTT
PRIMER_LEFT_14=520,20
PRIMER_RIGHT_14=1312,20
PRIMER_LEFT_14_TM=57.265
PRIMER_RIGHT_14_TM=56.715
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=7.2000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=793
PRIMER_PAIR_15_PENALTY=6.072263
PRIMER_LEFT_15_PENALTY=4.165121
PRIMER_RIGHT_15_PENALTY=1.907142
PRIMER_LEFT_15_SEQUENCE=GGACATGAGATTTTGCGTAG
PRIMER_RIGHT_15_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_15=522,20
PRIMER_RIGHT_15=1313,20
PRIMER_LEFT_15_TM=55.835
PRIMER_RIGHT_15_TM=58.093
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.4000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=792
PRIMER_PAIR_16_PENALTY=6.078039
PRIMER_LEFT_16_PENALTY=4.170896
PRIMER_RIGHT_16_PENALTY=1.907142
PRIMER_LEFT_16_SEQUENCE=GGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_16_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_16=522,21
PRIMER_RIGHT_16=1313,20
PRIMER_LEFT_16_TM=56.829
PRIMER_RIGHT_16_TM=58.093
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.1000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=792
PRIMER_PAIR_17_PENALTY=6.252586
PRIMER_LEFT_17_PENALTY=2.967917
PRIMER_RIGHT_17_PENALTY=3.284668
PRIMER_LEFT_17_SEQUENCE=AAATACGATGCGTGGCTGCT
PRIMER_RIGHT_17_SEQUENCE=GCAATACTCGTTCCCATTTT
PRIMER_LEFT_17=314,20
PRIMER_RIGHT_17=1312,20
PRIMER_LEFT_17_TM=62.968
PRIMER_RIGHT_17_TM=56.715
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=999
PRIMER_PAIR_18_PENALTY=6.312666
PRIMER_LEFT_18_PENALTY=4.013323
PRIMER_RIGHT_18_PENALTY=2.299343
PRIMER_LEFT_18_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_18_SEQUENCE=GATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_18=521,21
PRIMER_RIGHT_18=1429,22
PRIMER_LEFT_18_TM=56.987
PRIMER_RIGHT_18_TM=59.701
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=909
PRIMER_PAIR_19_PENALTY=6.407052
PRIMER_LEFT_19_PENALTY=4.107708
PRIMER_RIGHT_19_PENALTY=2.299343
PRIMER_LEFT_19_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_19_SEQUENCE=GATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_19=521,22
PRIMER_RIGHT_19=1429,22
PRIMER_LEFT_19_TM=57.892
PRIMER_RIGHT_19_TM=59.701
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.1000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=909
PRIMER_PAIR_20_PENALTY=6.434388
PRIMER_LEFT_20_PENALTY=4.013323
PRIMER_RIGHT_20_PENALTY=2.421065
PRIMER_LEFT_20_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_20_SEQUENCE=TATAATGCTCTTGGGGTTGG
PRIMER_LEFT_20=521,21
PRIMER_RIGHT_20=1427,20
PRIMER_LEFT_20_TM=56.987
PRIMER_RIGHT_20_TM=57.579
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=907
PRIMER_PAIR_21_PENALTY=6.464464
PRIMER_LEFT_21_PENALTY=4.165121
PRIMER_RIGHT_21_PENALTY=2.299343
PRIMER_LEFT_21_SEQUENCE=GGACATGAGATTTTGCGTAG
PRIMER_RIGHT_21_SEQUENCE=GATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_21=522,20
PRIMER_RIGHT_21=1429,22
PRIMER_LEFT_21_TM=55.835
PRIMER_RIGHT_21_TM=59.701
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.4000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=908
PRIMER_PAIR_22_PENALTY=6.470240
PRIMER_LEFT_22_PENALTY=4.170896
PRIMER_RIGHT_22_PENALTY=2.299343
PRIMER_LEFT_22_SEQUENCE=GGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_22_SEQUENCE=GATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_22=522,21
PRIMER_RIGHT_22=1429,22
PRIMER_LEFT_22_TM=56.829
PRIMER_RIGHT_22_TM=59.701
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.1000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=908
PRIMER_PAIR_23_PENALTY=6.522069
PRIMER_LEFT_23_PENALTY=3.549940
PRIMER_RIGHT_23_PENALTY=2.972129
PRIMER_LEFT_23_SEQUENCE=AATACGATGCGTGGCTGCTC
PRIMER_RIGHT_23_SEQUENCE=ATGCAATACTCGTTCCCATT
PRIMER_LEFT_23=315,20
PRIMER_RIGHT_23=1314,20
PRIMER_LEFT_23_TM=63.550
PRIMER_RIGHT_23_TM=57.028
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=1000
PRIMER_PAIR_24_PENALTY=6.528774
PRIMER_LEFT_24_PENALTY=4.107708
PRIMER_RIGHT_24_PENALTY=2.421065
PRIMER_LEFT_24_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_24_SEQUENCE=TATAATGCTCTTGGGGTTGG
PRIMER_LEFT_24=521,22
PRIMER_RIGHT_24=1427,20
PRIMER_LEFT_24_TM=57.892
PRIMER_RIGHT_24_TM=57.579
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=5.1000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=907
PRIMER_PAIR_25_PENALTY=6.537086
PRIMER_LEFT_25_PENALTY=4.629943
PRIMER_RIGHT_25_PENALTY=1.907142
PRIMER_LEFT_25_SEQUENCE=CTAAGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_25_SEQUENCE=AAATGCAATACTCGTTCCCA
PRIMER_LEFT_25=518,24
PRIMER_RIGHT_25=1316,20
PRIMER_LEFT_25_TM=59.370
PRIMER_RIGHT_25_TM=58.093
PRIMER_LEFT_25_GC_PERCENT=41.667
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.4000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=799
PRIMER_PAIR_26_PENALTY=6.586186
PRIMER_LEFT_26_PENALTY=4.165121
PRIMER_RIGHT_26_PENALTY=2.421065
PRIMER_LEFT_26_SEQUENCE=GGACATGAGATTTTGCGTAG
PRIMER_RIGHT_26_SEQUENCE=TATAATGCTCTTGGGGTTGG
PRIMER_LEFT_26=522,20
PRIMER_RIGHT_26=1427,20
PRIMER_LEFT_26_TM=55.835
PRIMER_RIGHT_26_TM=57.579
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=906
PRIMER_PAIR_27_PENALTY=6.591962
PRIMER_LEFT_27_PENALTY=4.170896
PRIMER_RIGHT_27_PENALTY=2.421065
PRIMER_LEFT_27_SEQUENCE=GGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_27_SEQUENCE=TATAATGCTCTTGGGGTTGG
PRIMER_LEFT_27=522,21
PRIMER_RIGHT_27=1427,20
PRIMER_LEFT_27_TM=56.829
PRIMER_RIGHT_27_TM=57.579
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.1000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=906
PRIMER_PAIR_28_PENALTY=6.631112
PRIMER_LEFT_28_PENALTY=3.597306
PRIMER_RIGHT_28_PENALTY=3.033807
PRIMER_LEFT_28_SEQUENCE=AAGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_28_SEQUENCE=TGATATAATGCTCTTGGGGTTG
PRIMER_LEFT_28=520,22
PRIMER_RIGHT_28=1430,22
PRIMER_LEFT_28_TM=58.403
PRIMER_RIGHT_28_TM=58.966
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.4000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=911
PRIMER_PAIR_29_PENALTY=6.631112
PRIMER_LEFT_29_PENALTY=3.597306
PRIMER_RIGHT_29_PENALTY=3.033807
PRIMER_LEFT_29_SEQUENCE=CTAAGGACATGAGATTTTGCGT
PRIMER_RIGHT_29_SEQUENCE=TGATATAATGCTCTTGGGGTTG
PRIMER_LEFT_29=518,22
PRIMER_RIGHT_29=1430,22
PRIMER_LEFT_29_TM=58.403
PRIMER_RIGHT_29_TM=58.966
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=913
PRIMER_PAIR_30_PENALTY=6.635560
PRIMER_LEFT_30_PENALTY=3.597306
PRIMER_RIGHT_30_PENALTY=3.038254
PRIMER_LEFT_30_SEQUENCE=AAGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_30_SEQUENCE=ATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_30=520,22
PRIMER_RIGHT_30=1428,21
PRIMER_LEFT_30_TM=58.403
PRIMER_RIGHT_30_TM=57.962
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.4000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=909
PRIMER_PAIR_31_PENALTY=6.834609
PRIMER_LEFT_31_PENALTY=3.549940
PRIMER_RIGHT_31_PENALTY=3.284668
PRIMER_LEFT_31_SEQUENCE=AATACGATGCGTGGCTGCTC
PRIMER_RIGHT_31_SEQUENCE=GCAATACTCGTTCCCATTTT
PRIMER_LEFT_31=315,20
PRIMER_RIGHT_31=1312,20
PRIMER_LEFT_31_TM=63.550
PRIMER_RIGHT_31_TM=56.715
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=998
PRIMER_PAIR_32_PENALTY=6.881974
PRIMER_LEFT_32_PENALTY=3.597306
PRIMER_RIGHT_32_PENALTY=3.284668
PRIMER_LEFT_32_SEQUENCE=AAGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_32_SEQUENCE=GCAATACTCGTTCCCATTTT
PRIMER_LEFT_32=520,22
PRIMER_RIGHT_32=1312,20
PRIMER_LEFT_32_TM=58.403
PRIMER_RIGHT_32_TM=56.715
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.4000
PRIMER_RIGHT_32_END_STABILITY=7.2000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=793
PRIMER_PAIR_33_PENALTY=6.985452
PRIMER_LEFT_33_PENALTY=4.013323
PRIMER_RIGHT_33_PENALTY=2.972129
PRIMER_LEFT_33_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_33_SEQUENCE=ATGCAATACTCGTTCCCATT
PRIMER_LEFT_33=521,21
PRIMER_RIGHT_33=1314,20
PRIMER_LEFT_33_TM=56.987
PRIMER_RIGHT_33_TM=57.028
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.4000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=794
PRIMER_PAIR_34_PENALTY=6.996051
PRIMER_LEFT_34_PENALTY=5.088909
PRIMER_RIGHT_34_PENALTY=1.907142
PRIMER_LEFT_34_SEQUENCE=CTAAGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_34_SEQUENCE=AAATGCAATACTCGTTCCCA
PRIMER_LEFT_34=518,25
PRIMER_RIGHT_34=1316,20
PRIMER_LEFT_34_TM=60.089
PRIMER_RIGHT_34_TM=58.093
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=5.1000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=799
PRIMER_PAIR_35_PENALTY=7.047130
PRIMER_LEFT_35_PENALTY=4.013323
PRIMER_RIGHT_35_PENALTY=3.033807
PRIMER_LEFT_35_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_35_SEQUENCE=TGATATAATGCTCTTGGGGTTG
PRIMER_LEFT_35=521,21
PRIMER_RIGHT_35=1430,22
PRIMER_LEFT_35_TM=56.987
PRIMER_RIGHT_35_TM=58.966
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.4000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=910
PRIMER_PAIR_36_PENALTY=7.051577
PRIMER_LEFT_36_PENALTY=4.013323
PRIMER_RIGHT_36_PENALTY=3.038254
PRIMER_LEFT_36_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_36_SEQUENCE=ATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_36=521,21
PRIMER_RIGHT_36=1428,21
PRIMER_LEFT_36_TM=56.987
PRIMER_RIGHT_36_TM=57.962
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.4000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=908
PRIMER_PAIR_37_PENALTY=7.079837
PRIMER_LEFT_37_PENALTY=4.107708
PRIMER_RIGHT_37_PENALTY=2.972129
PRIMER_LEFT_37_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_37_SEQUENCE=ATGCAATACTCGTTCCCATT
PRIMER_LEFT_37=521,22
PRIMER_RIGHT_37=1314,20
PRIMER_LEFT_37_TM=57.892
PRIMER_RIGHT_37_TM=57.028
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.1000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=794
PRIMER_PAIR_38_PENALTY=7.095185
PRIMER_LEFT_38_PENALTY=5.188042
PRIMER_RIGHT_38_PENALTY=1.907142
PRIMER_LEFT_38_SEQUENCE=GGAAGAGTTAATACTGTTTCTCGCA
PRIMER_RIGHT_38_SEQUENCE=TGCAATACTCGTTCCCATTT
PRIMER_LEFT_38=563,25
PRIMER_RIGHT_38=1313,20
PRIMER_LEFT_38_TM=60.188
PRIMER_RIGHT_38_TM=58.093
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=10.2000
PRIMER_RIGHT_38_END_STABILITY=7.2000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=751
PRIMER_PAIR_39_PENALTY=7.095185
PRIMER_LEFT_39_PENALTY=5.188042
PRIMER_RIGHT_39_PENALTY=1.907142
PRIMER_LEFT_39_SEQUENCE=GGAAGAGTTAATACTGTTTCTCGCA
PRIMER_RIGHT_39_SEQUENCE=AAATGCAATACTCGTTCCCA
PRIMER_LEFT_39=563,25
PRIMER_RIGHT_39=1316,20
PRIMER_LEFT_39_TM=60.188
PRIMER_RIGHT_39_TM=58.093
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=10.2000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=754
PRIMER_PAIR_40_PENALTY=7.137250
PRIMER_LEFT_40_PENALTY=4.165121
PRIMER_RIGHT_40_PENALTY=2.972129
PRIMER_LEFT_40_SEQUENCE=GGACATGAGATTTTGCGTAG
PRIMER_RIGHT_40_SEQUENCE=ATGCAATACTCGTTCCCATT
PRIMER_LEFT_40=522,20
PRIMER_RIGHT_40=1314,20
PRIMER_LEFT_40_TM=55.835
PRIMER_RIGHT_40_TM=57.028
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.4000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=793
PRIMER_PAIR_41_PENALTY=7.141515
PRIMER_LEFT_41_PENALTY=4.107708
PRIMER_RIGHT_41_PENALTY=3.033807
PRIMER_LEFT_41_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_41_SEQUENCE=TGATATAATGCTCTTGGGGTTG
PRIMER_LEFT_41=521,22
PRIMER_RIGHT_41=1430,22
PRIMER_LEFT_41_TM=57.892
PRIMER_RIGHT_41_TM=58.966
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=5.1000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=910
PRIMER_PAIR_42_PENALTY=7.143025
PRIMER_LEFT_42_PENALTY=4.170896
PRIMER_RIGHT_42_PENALTY=2.972129
PRIMER_LEFT_42_SEQUENCE=GGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_42_SEQUENCE=ATGCAATACTCGTTCCCATT
PRIMER_LEFT_42=522,21
PRIMER_RIGHT_42=1314,20
PRIMER_LEFT_42_TM=56.829
PRIMER_RIGHT_42_TM=57.028
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=5.1000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=793
PRIMER_PAIR_43_PENALTY=7.145962
PRIMER_LEFT_43_PENALTY=4.107708
PRIMER_RIGHT_43_PENALTY=3.038254
PRIMER_LEFT_43_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_43_SEQUENCE=ATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_43=521,22
PRIMER_RIGHT_43=1428,21
PRIMER_LEFT_43_TM=57.892
PRIMER_RIGHT_43_TM=57.962
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=5.1000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=908
PRIMER_PAIR_44_PENALTY=7.198927
PRIMER_LEFT_44_PENALTY=4.165121
PRIMER_RIGHT_44_PENALTY=3.033807
PRIMER_LEFT_44_SEQUENCE=GGACATGAGATTTTGCGTAG
PRIMER_RIGHT_44_SEQUENCE=TGATATAATGCTCTTGGGGTTG
PRIMER_LEFT_44=522,20
PRIMER_RIGHT_44=1430,22
PRIMER_LEFT_44_TM=55.835
PRIMER_RIGHT_44_TM=58.966
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.4000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=909
PRIMER_PAIR_45_PENALTY=7.203374
PRIMER_LEFT_45_PENALTY=4.165121
PRIMER_RIGHT_45_PENALTY=3.038254
PRIMER_LEFT_45_SEQUENCE=GGACATGAGATTTTGCGTAG
PRIMER_RIGHT_45_SEQUENCE=ATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_45=522,20
PRIMER_RIGHT_45=1428,21
PRIMER_LEFT_45_TM=55.835
PRIMER_RIGHT_45_TM=57.962
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=907
PRIMER_PAIR_46_PENALTY=7.204703
PRIMER_LEFT_46_PENALTY=4.170896
PRIMER_RIGHT_46_PENALTY=3.033807
PRIMER_LEFT_46_SEQUENCE=GGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_46_SEQUENCE=TGATATAATGCTCTTGGGGTTG
PRIMER_LEFT_46=522,21
PRIMER_RIGHT_46=1430,22
PRIMER_LEFT_46_TM=56.829
PRIMER_RIGHT_46_TM=58.966
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=5.1000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=909
PRIMER_PAIR_47_PENALTY=7.209150
PRIMER_LEFT_47_PENALTY=4.170896
PRIMER_RIGHT_47_PENALTY=3.038254
PRIMER_LEFT_47_SEQUENCE=GGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_47_SEQUENCE=ATATAATGCTCTTGGGGTTGG
PRIMER_LEFT_47=522,21
PRIMER_RIGHT_47=1428,21
PRIMER_LEFT_47_TM=56.829
PRIMER_RIGHT_47_TM=57.962
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=5.1000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=907
PRIMER_PAIR_48_PENALTY=7.297992
PRIMER_LEFT_48_PENALTY=4.013323
PRIMER_RIGHT_48_PENALTY=3.284668
PRIMER_LEFT_48_SEQUENCE=AGGACATGAGATTTTGCGTAG
PRIMER_RIGHT_48_SEQUENCE=GCAATACTCGTTCCCATTTT
PRIMER_LEFT_48=521,21
PRIMER_RIGHT_48=1312,20
PRIMER_LEFT_48_TM=56.987
PRIMER_RIGHT_48_TM=56.715
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.4000
PRIMER_RIGHT_48_END_STABILITY=7.2000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=792
PRIMER_PAIR_49_PENALTY=7.392377
PRIMER_LEFT_49_PENALTY=4.107708
PRIMER_RIGHT_49_PENALTY=3.284668
PRIMER_LEFT_49_SEQUENCE=AGGACATGAGATTTTGCGTAGT
PRIMER_RIGHT_49_SEQUENCE=GCAATACTCGTTCCCATTTT
PRIMER_LEFT_49=521,22
PRIMER_RIGHT_49=1312,20
PRIMER_LEFT_49_TM=57.892
PRIMER_RIGHT_49_TM=56.715
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.1000
PRIMER_RIGHT_49_END_STABILITY=7.2000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=792
=
SEQUENCE_ID=MAGIC.149_3_16852386_16852831_hi-0_ct-1_168608_1569340_TRUE
SEQUENCE_TARGET=950,545
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14673044_14673131_tsu-0_wil-2_24051_39637_TRUE
SEQUENCE_TARGET=950,187
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_14637520_14637672_zu-0_no-0_23640_918146_TRUE
SEQUENCE_TARGET=950,252
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_12774737_12774815_no-0_ct-1_2996_841268_TRUE
SEQUENCE_TARGET=950,178
SEQUENCE_TEMPLATE=ATTATGACACACATGATTATGTACGAATGATACAGACCAATTNTGAACATANTATTNATGACTGTTTTTGTAAACANTGGTTAACGGACTTTGAGTCTTTGAGANTTTGACCACATCATTAAGCTAATATTTTTNTCCNTTGTCAATCAAAATATATTGAACCAAAATTCTANACATCAACAAGCAACAAAAGCCAAACGATGAAGGTTATCAAATTCTTNTTATGNAATCAACAANTNTTCACATTCATTTANGGTAAAGCTAATTTTCACCTAAATTATAAACTTACTTGACTTTNAAAANAAGTTGCACNTTGTTGGNTTGCTGGCTGCTNCTGTTTGAGAAGAAGAAAACTAAAAAGGTACGTAAGATGAANTTNTTTTTTTNNTTAATTCAATTTGGTTANGTTAAAAATTNGGCNANCTTTNTTTCTAATTTACAACAAATAAAACTTTTAAATATGATATANATTCTTTTATTATNGGCTAATGGGCTTANGATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATACAAACCCAATAANNTTAAGGAAAATTATAAAATTTNGGCAGNTCAGCTGACCACCCATCAATACATGTGGATCCGCCNTTGATCANTGATGATGCTCACAAATTAAAATCTTAACCNAACTCTTAACTNATAAATTTTNANAATCTNTTCATAAAATNNNNNNNAGAGNCNTTGTTAATAACCTCCNATTGANGATGGTCTNGNNNTNTGATGTTGGCAGAGGGCGAAACAATTANANATGGCGAGATAATGGNTATGGAAGGGCATGTCAGATANTNAATCNAGCCTTGAGGATANGGCTGTTTTTGAGCGGTCANCAATGATACNAATACTTCATAATATATGATTTAGGATATAGTTTCGATTTTCTTANTTACTTTTNCTTGTCTTTTACTTTNACATTTTATTATAAATAAGGGTTANNGTTTGTTTGTGNAACCTATCCAAGCAATTGAGAGCTTTGNAAGATTCTANCGGATTANGTCGAGGGAAGATAACGTANTTNTCTTTNGGGAGTTTNTACTCTTGTTACTTTATTGTTCNATNTTTCTNCTNTCATAATAANGGTTTNGGTTTTGAGTTGGATTCTACCTCTAACAGTTGCTAATATTNTTACTGTATAAAAAGACATATGCGAAATAATATTTAGNGNTACTCNNTTTTCTTAATNTTTNATATCCATTCCAAAANTTGCTATGTTCAAAANCTATGACTCAGGTAAAATATATTNTTGGGTCCGCCNCTNATGCATGNCTTGGTTTAGCAATGAACTGTGAGAGAACTTGTACGGCATAACANANTTTTAANCGAATGATAAAAAATAAAAACCTACTAATTAATNGTCTNTAACTTTTAGGATCCTCAAACATAGGACTGTCCATCCTTCAAAAGCTTGTGGTTCTGTTCCATATGNGGCATTGGGGGTTCTTANCCAATAAACTTATCTCAATACTTATTTTCTTTGAGAACGTAAAATGTTGTANTTCTGATTGTTAAGAATTCAGGATAGCTACTAAGCACTATCGAGGTTTCTTTACANNTTTTTTTTGTCNTTTTTGTTTNATTANTGAATAGATGTGCCNTATAATATTNTTTGGTCGTAGGTNTNACTCATGAAGAATTTCAATGCAGAAACACCAAACTACATTATATGAACCAACTTGCTTATANTACGAATAAACACAAAATTACATAGTGGACAAAAAGTGGCCACATTTGAAGCACTGGAGTTGAGCAAAGATCTCATAACTCGCAATCTGCAAACANNNNNNNNNNNNNNTCCTNTTTTTGTCNCATATCCCCTTNNTTTTTTNATGAAATGTGTATAGACGANNGNGTAGAAACTGNCTCTCNCNCTCTNCTCTNNNCTCTCTCTCCNCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.126457
PRIMER_LEFT_0_PENALTY=0.626296
PRIMER_RIGHT_0_PENALTY=1.500160
PRIMER_LEFT_0_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_0_SEQUENCE=TGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_0=622,20
PRIMER_RIGHT_0=1368,21
PRIMER_LEFT_0_TM=59.374
PRIMER_RIGHT_0_TM=59.500
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=747
PRIMER_PAIR_1_PENALTY=2.823436
PRIMER_LEFT_1_PENALTY=0.626296
PRIMER_RIGHT_1_PENALTY=2.197140
PRIMER_LEFT_1_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_1_SEQUENCE=ATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_1=622,20
PRIMER_RIGHT_1=1369,22
PRIMER_LEFT_1_TM=59.374
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=748
PRIMER_PAIR_2_PENALTY=2.890553
PRIMER_LEFT_2_PENALTY=1.390393
PRIMER_RIGHT_2_PENALTY=1.500160
PRIMER_LEFT_2_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_2_SEQUENCE=TGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_2=621,21
PRIMER_RIGHT_2=1368,21
PRIMER_LEFT_2_TM=60.390
PRIMER_RIGHT_2_TM=59.500
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=748
PRIMER_PAIR_3_PENALTY=2.890553
PRIMER_LEFT_3_PENALTY=1.390393
PRIMER_RIGHT_3_PENALTY=1.500160
PRIMER_LEFT_3_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_3_SEQUENCE=TGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_3=620,21
PRIMER_RIGHT_3=1368,21
PRIMER_LEFT_3_TM=60.390
PRIMER_RIGHT_3_TM=59.500
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=749
PRIMER_PAIR_4_PENALTY=3.587532
PRIMER_LEFT_4_PENALTY=1.390393
PRIMER_RIGHT_4_PENALTY=2.197140
PRIMER_LEFT_4_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_4_SEQUENCE=ATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_4=621,21
PRIMER_RIGHT_4=1369,22
PRIMER_LEFT_4_TM=60.390
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=749
PRIMER_PAIR_5_PENALTY=3.587532
PRIMER_LEFT_5_PENALTY=1.390393
PRIMER_RIGHT_5_PENALTY=2.197140
PRIMER_LEFT_5_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_5_SEQUENCE=ATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_5=620,21
PRIMER_RIGHT_5=1369,22
PRIMER_LEFT_5_TM=60.390
PRIMER_RIGHT_5_TM=59.803
PRIMER_LEFT_5_GC_PERCENT=52.381
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=5.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=750
PRIMER_PAIR_6_PENALTY=3.759284
PRIMER_LEFT_6_PENALTY=0.626296
PRIMER_RIGHT_6_PENALTY=3.132987
PRIMER_LEFT_6_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_6_SEQUENCE=GCCGTACAAGTTCTCTCACAGTT
PRIMER_LEFT_6=622,20
PRIMER_RIGHT_6=1367,23
PRIMER_LEFT_6_TM=59.374
PRIMER_RIGHT_6_TM=59.867
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=746
PRIMER_PAIR_7_PENALTY=3.805717
PRIMER_LEFT_7_PENALTY=0.626296
PRIMER_RIGHT_7_PENALTY=3.179421
PRIMER_LEFT_7_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_7_SEQUENCE=TATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_7=622,20
PRIMER_RIGHT_7=1370,23
PRIMER_LEFT_7_TM=59.374
PRIMER_RIGHT_7_TM=59.821
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=749
PRIMER_PAIR_8_PENALTY=4.051714
PRIMER_LEFT_8_PENALTY=2.551554
PRIMER_RIGHT_8_PENALTY=1.500160
PRIMER_LEFT_8_SEQUENCE=AGCTGACCACCCATCAATAC
PRIMER_RIGHT_8_SEQUENCE=TGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_8=621,20
PRIMER_RIGHT_8=1368,21
PRIMER_LEFT_8_TM=57.448
PRIMER_RIGHT_8_TM=59.500
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=5.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=748
PRIMER_PAIR_9_PENALTY=4.173363
PRIMER_LEFT_9_PENALTY=0.626296
PRIMER_RIGHT_9_PENALTY=3.547067
PRIMER_LEFT_9_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_9_SEQUENCE=GCCGTACAAGTTCTCTCACA
PRIMER_LEFT_9=622,20
PRIMER_RIGHT_9=1367,20
PRIMER_LEFT_9_TM=59.374
PRIMER_RIGHT_9_TM=56.453
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=5.6000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=746
PRIMER_PAIR_10_PENALTY=4.173363
PRIMER_LEFT_10_PENALTY=0.626296
PRIMER_RIGHT_10_PENALTY=3.547067
PRIMER_LEFT_10_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_10_SEQUENCE=TGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_10=622,20
PRIMER_RIGHT_10=1368,20
PRIMER_LEFT_10_TM=59.374
PRIMER_RIGHT_10_TM=56.453
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=747
PRIMER_PAIR_11_PENALTY=4.523380
PRIMER_LEFT_11_PENALTY=1.390393
PRIMER_RIGHT_11_PENALTY=3.132987
PRIMER_LEFT_11_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_11_SEQUENCE=GCCGTACAAGTTCTCTCACAGTT
PRIMER_LEFT_11=621,21
PRIMER_RIGHT_11=1367,23
PRIMER_LEFT_11_TM=60.390
PRIMER_RIGHT_11_TM=59.867
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=5.6000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=747
PRIMER_PAIR_12_PENALTY=4.523380
PRIMER_LEFT_12_PENALTY=1.390393
PRIMER_RIGHT_12_PENALTY=3.132987
PRIMER_LEFT_12_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_12_SEQUENCE=GCCGTACAAGTTCTCTCACAGTT
PRIMER_LEFT_12=620,21
PRIMER_RIGHT_12=1367,23
PRIMER_LEFT_12_TM=60.390
PRIMER_RIGHT_12_TM=59.867
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=5.6000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=748
PRIMER_PAIR_13_PENALTY=4.569813
PRIMER_LEFT_13_PENALTY=1.390393
PRIMER_RIGHT_13_PENALTY=3.179421
PRIMER_LEFT_13_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_13_SEQUENCE=TATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_13=621,21
PRIMER_RIGHT_13=1370,23
PRIMER_LEFT_13_TM=60.390
PRIMER_RIGHT_13_TM=59.821
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=5.6000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=750
PRIMER_PAIR_14_PENALTY=4.569813
PRIMER_LEFT_14_PENALTY=1.390393
PRIMER_RIGHT_14_PENALTY=3.179421
PRIMER_LEFT_14_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_14_SEQUENCE=TATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_14=620,21
PRIMER_RIGHT_14=1370,23
PRIMER_LEFT_14_TM=60.390
PRIMER_RIGHT_14_TM=59.821
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=5.6000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=751
PRIMER_PAIR_15_PENALTY=4.641089
PRIMER_LEFT_15_PENALTY=0.626296
PRIMER_RIGHT_15_PENALTY=4.014792
PRIMER_LEFT_15_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_15_SEQUENCE=CAAGTTCTCTCACAGTTCATTGCT
PRIMER_LEFT_15=622,20
PRIMER_RIGHT_15=1361,24
PRIMER_LEFT_15_TM=59.374
PRIMER_RIGHT_15_TM=59.985
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=41.667
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=5.6000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=740
PRIMER_PAIR_16_PENALTY=4.690924
PRIMER_LEFT_16_PENALTY=0.626296
PRIMER_RIGHT_16_PENALTY=4.064628
PRIMER_LEFT_16_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_16_SEQUENCE=ATGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_16=622,20
PRIMER_RIGHT_16=1369,21
PRIMER_LEFT_16_TM=59.374
PRIMER_RIGHT_16_TM=56.935
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.6000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=748
PRIMER_PAIR_17_PENALTY=4.704412
PRIMER_LEFT_17_PENALTY=0.626296
PRIMER_RIGHT_17_PENALTY=4.078115
PRIMER_LEFT_17_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_17_SEQUENCE=ATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_17=622,20
PRIMER_RIGHT_17=1369,20
PRIMER_LEFT_17_TM=59.374
PRIMER_RIGHT_17_TM=55.922
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=5.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=748
PRIMER_PAIR_18_PENALTY=4.748694
PRIMER_LEFT_18_PENALTY=2.551554
PRIMER_RIGHT_18_PENALTY=2.197140
PRIMER_LEFT_18_SEQUENCE=AGCTGACCACCCATCAATAC
PRIMER_RIGHT_18_SEQUENCE=ATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_18=621,20
PRIMER_RIGHT_18=1369,22
PRIMER_LEFT_18_TM=57.448
PRIMER_RIGHT_18_TM=59.803
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=5.6000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=749
PRIMER_PAIR_19_PENALTY=4.800953
PRIMER_LEFT_19_PENALTY=0.626296
PRIMER_RIGHT_19_PENALTY=4.174657
PRIMER_LEFT_19_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_19_SEQUENCE=TCTCTCACAGTTCATTGCTAAACC
PRIMER_LEFT_19=622,20
PRIMER_RIGHT_19=1356,24
PRIMER_LEFT_19_TM=59.374
PRIMER_RIGHT_19_TM=59.825
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=41.667
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=735
PRIMER_PAIR_20_PENALTY=4.937459
PRIMER_LEFT_20_PENALTY=1.390393
PRIMER_RIGHT_20_PENALTY=3.547067
PRIMER_LEFT_20_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_20_SEQUENCE=GCCGTACAAGTTCTCTCACA
PRIMER_LEFT_20=621,21
PRIMER_RIGHT_20=1367,20
PRIMER_LEFT_20_TM=60.390
PRIMER_RIGHT_20_TM=56.453
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=5.6000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=747
PRIMER_PAIR_21_PENALTY=4.937459
PRIMER_LEFT_21_PENALTY=1.390393
PRIMER_RIGHT_21_PENALTY=3.547067
PRIMER_LEFT_21_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_21_SEQUENCE=TGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_21=621,21
PRIMER_RIGHT_21=1368,20
PRIMER_LEFT_21_TM=60.390
PRIMER_RIGHT_21_TM=56.453
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=5.6000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=748
PRIMER_PAIR_22_PENALTY=4.937459
PRIMER_LEFT_22_PENALTY=1.390393
PRIMER_RIGHT_22_PENALTY=3.547067
PRIMER_LEFT_22_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_22_SEQUENCE=GCCGTACAAGTTCTCTCACA
PRIMER_LEFT_22=620,21
PRIMER_RIGHT_22=1367,20
PRIMER_LEFT_22_TM=60.390
PRIMER_RIGHT_22_TM=56.453
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=5.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=748
PRIMER_PAIR_23_PENALTY=4.937459
PRIMER_LEFT_23_PENALTY=1.390393
PRIMER_RIGHT_23_PENALTY=3.547067
PRIMER_LEFT_23_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_23_SEQUENCE=TGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_23=620,21
PRIMER_RIGHT_23=1368,20
PRIMER_LEFT_23_TM=60.390
PRIMER_RIGHT_23_TM=56.453
PRIMER_LEFT_23_GC_PERCENT=52.381
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=5.6000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=749
PRIMER_PAIR_24_PENALTY=5.025466
PRIMER_LEFT_24_PENALTY=0.626296
PRIMER_RIGHT_24_PENALTY=4.399169
PRIMER_LEFT_24_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_24_SEQUENCE=TTATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_24=622,20
PRIMER_RIGHT_24=1371,22
PRIMER_LEFT_24_TM=59.374
PRIMER_RIGHT_24_TM=57.601
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=5.6000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=750
PRIMER_PAIR_25_PENALTY=5.162434
PRIMER_LEFT_25_PENALTY=0.626296
PRIMER_RIGHT_25_PENALTY=4.536138
PRIMER_LEFT_25_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_25_SEQUENCE=TTATGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_25=622,20
PRIMER_RIGHT_25=1371,23
PRIMER_LEFT_25_TM=59.374
PRIMER_RIGHT_25_TM=58.464
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=5.6000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=750
PRIMER_PAIR_26_PENALTY=5.162434
PRIMER_LEFT_26_PENALTY=0.626296
PRIMER_RIGHT_26_PENALTY=4.536138
PRIMER_LEFT_26_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_26_SEQUENCE=GTTATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_26=622,20
PRIMER_RIGHT_26=1372,23
PRIMER_LEFT_26_TM=59.374
PRIMER_RIGHT_26_TM=58.464
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=5.6000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=751
PRIMER_PAIR_27_PENALTY=5.162434
PRIMER_LEFT_27_PENALTY=0.626296
PRIMER_RIGHT_27_PENALTY=4.536138
PRIMER_LEFT_27_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_27_SEQUENCE=TGTTATGCCGTACAAGTTCTCTC
PRIMER_LEFT_27=622,20
PRIMER_RIGHT_27=1373,23
PRIMER_LEFT_27_TM=59.374
PRIMER_RIGHT_27_TM=58.464
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.6000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=752
PRIMER_PAIR_28_PENALTY=5.360862
PRIMER_LEFT_28_PENALTY=0.626296
PRIMER_RIGHT_28_PENALTY=4.734566
PRIMER_LEFT_28_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_28_SEQUENCE=GTTATGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_28=622,20
PRIMER_RIGHT_28=1372,24
PRIMER_LEFT_28_TM=59.374
PRIMER_RIGHT_28_TM=59.265
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.833
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=5.6000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=751
PRIMER_PAIR_29_PENALTY=5.366205
PRIMER_LEFT_29_PENALTY=0.626296
PRIMER_RIGHT_29_PENALTY=4.739909
PRIMER_LEFT_29_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_29_SEQUENCE=CCGTACAAGTTCTCTCACAGTTCA
PRIMER_LEFT_29=622,20
PRIMER_RIGHT_29=1366,24
PRIMER_LEFT_29_TM=59.374
PRIMER_RIGHT_29_TM=60.740
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.833
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=5.6000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=745
PRIMER_PAIR_30_PENALTY=5.405185
PRIMER_LEFT_30_PENALTY=1.390393
PRIMER_RIGHT_30_PENALTY=4.014792
PRIMER_LEFT_30_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_30_SEQUENCE=CAAGTTCTCTCACAGTTCATTGCT
PRIMER_LEFT_30=621,21
PRIMER_RIGHT_30=1361,24
PRIMER_LEFT_30_TM=60.390
PRIMER_RIGHT_30_TM=59.985
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=41.667
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=5.6000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=741
PRIMER_PAIR_31_PENALTY=5.405185
PRIMER_LEFT_31_PENALTY=1.390393
PRIMER_RIGHT_31_PENALTY=4.014792
PRIMER_LEFT_31_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_31_SEQUENCE=CAAGTTCTCTCACAGTTCATTGCT
PRIMER_LEFT_31=620,21
PRIMER_RIGHT_31=1361,24
PRIMER_LEFT_31_TM=60.390
PRIMER_RIGHT_31_TM=59.985
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=41.667
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.6000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=742
PRIMER_PAIR_32_PENALTY=5.455020
PRIMER_LEFT_32_PENALTY=1.390393
PRIMER_RIGHT_32_PENALTY=4.064628
PRIMER_LEFT_32_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_32_SEQUENCE=ATGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_32=621,21
PRIMER_RIGHT_32=1369,21
PRIMER_LEFT_32_TM=60.390
PRIMER_RIGHT_32_TM=56.935
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=5.6000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=749
PRIMER_PAIR_33_PENALTY=5.455020
PRIMER_LEFT_33_PENALTY=1.390393
PRIMER_RIGHT_33_PENALTY=4.064628
PRIMER_LEFT_33_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_33_SEQUENCE=ATGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_33=620,21
PRIMER_RIGHT_33=1369,21
PRIMER_LEFT_33_TM=60.390
PRIMER_RIGHT_33_TM=56.935
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=5.6000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=750
PRIMER_PAIR_34_PENALTY=5.468508
PRIMER_LEFT_34_PENALTY=1.390393
PRIMER_RIGHT_34_PENALTY=4.078115
PRIMER_LEFT_34_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_34_SEQUENCE=ATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_34=621,21
PRIMER_RIGHT_34=1369,20
PRIMER_LEFT_34_TM=60.390
PRIMER_RIGHT_34_TM=55.922
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=5.6000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=749
PRIMER_PAIR_35_PENALTY=5.468508
PRIMER_LEFT_35_PENALTY=1.390393
PRIMER_RIGHT_35_PENALTY=4.078115
PRIMER_LEFT_35_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_35_SEQUENCE=ATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_35=620,21
PRIMER_RIGHT_35=1369,20
PRIMER_LEFT_35_TM=60.390
PRIMER_RIGHT_35_TM=55.922
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=5.6000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=750
PRIMER_PAIR_36_PENALTY=5.531930
PRIMER_LEFT_36_PENALTY=0.626296
PRIMER_RIGHT_36_PENALTY=4.905634
PRIMER_LEFT_36_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_36_SEQUENCE=TATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_36=622,20
PRIMER_RIGHT_36=1370,21
PRIMER_LEFT_36_TM=59.374
PRIMER_RIGHT_36_TM=56.094
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=5.6000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=749
PRIMER_PAIR_37_PENALTY=5.552718
PRIMER_LEFT_37_PENALTY=0.626296
PRIMER_RIGHT_37_PENALTY=4.926422
PRIMER_LEFT_37_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_37_SEQUENCE=CTCTCACAGTTCATTGCTAAACC
PRIMER_LEFT_37=622,20
PRIMER_RIGHT_37=1355,23
PRIMER_LEFT_37_TM=59.374
PRIMER_RIGHT_37_TM=58.074
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=43.478
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=5.6000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=734
PRIMER_PAIR_38_PENALTY=5.563683
PRIMER_LEFT_38_PENALTY=0.626296
PRIMER_RIGHT_38_PENALTY=4.937386
PRIMER_LEFT_38_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_38_SEQUENCE=TATGCCGTACAAGTTCTCTCAC
PRIMER_LEFT_38=622,20
PRIMER_RIGHT_38=1370,22
PRIMER_LEFT_38_TM=59.374
PRIMER_RIGHT_38_TM=57.063
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.6000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=749
PRIMER_PAIR_39_PENALTY=5.565049
PRIMER_LEFT_39_PENALTY=1.390393
PRIMER_RIGHT_39_PENALTY=4.174657
PRIMER_LEFT_39_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_39_SEQUENCE=TCTCTCACAGTTCATTGCTAAACC
PRIMER_LEFT_39=621,21
PRIMER_RIGHT_39=1356,24
PRIMER_LEFT_39_TM=60.390
PRIMER_RIGHT_39_TM=59.825
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=41.667
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=5.6000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=736
PRIMER_PAIR_40_PENALTY=5.565049
PRIMER_LEFT_40_PENALTY=1.390393
PRIMER_RIGHT_40_PENALTY=4.174657
PRIMER_LEFT_40_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_40_SEQUENCE=TCTCTCACAGTTCATTGCTAAACC
PRIMER_LEFT_40=620,21
PRIMER_RIGHT_40=1356,24
PRIMER_LEFT_40_TM=60.390
PRIMER_RIGHT_40_TM=59.825
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=41.667
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.6000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=737
PRIMER_PAIR_41_PENALTY=5.568802
PRIMER_LEFT_41_PENALTY=0.626296
PRIMER_RIGHT_41_PENALTY=4.942505
PRIMER_LEFT_41_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_41_SEQUENCE=CCGTACAAGTTCTCTCACAGTTC
PRIMER_LEFT_41=622,20
PRIMER_RIGHT_41=1366,23
PRIMER_LEFT_41_TM=59.374
PRIMER_RIGHT_41_TM=58.057
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.826
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=5.6000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=745
PRIMER_PAIR_42_PENALTY=5.593019
PRIMER_LEFT_42_PENALTY=0.626296
PRIMER_RIGHT_42_PENALTY=4.966722
PRIMER_LEFT_42_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_42_SEQUENCE=TCTCACAGTTCATTGCTAAACC
PRIMER_LEFT_42=622,20
PRIMER_RIGHT_42=1354,22
PRIMER_LEFT_42_TM=59.374
PRIMER_RIGHT_42_TM=57.033
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.6000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=733
PRIMER_PAIR_43_PENALTY=5.673099
PRIMER_LEFT_43_PENALTY=0.626296
PRIMER_RIGHT_43_PENALTY=5.046803
PRIMER_LEFT_43_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_43_SEQUENCE=TTATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_43=622,20
PRIMER_RIGHT_43=1371,24
PRIMER_LEFT_43_TM=59.374
PRIMER_RIGHT_43_TM=61.047
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=41.667
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=5.6000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=750
PRIMER_PAIR_44_PENALTY=5.673099
PRIMER_LEFT_44_PENALTY=0.626296
PRIMER_RIGHT_44_PENALTY=5.046803
PRIMER_LEFT_44_SEQUENCE=GCTGACCACCCATCAATACA
PRIMER_RIGHT_44_SEQUENCE=TGTTATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_44=622,20
PRIMER_RIGHT_44=1373,24
PRIMER_LEFT_44_TM=59.374
PRIMER_RIGHT_44_TM=61.047
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=5.6000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=752
PRIMER_PAIR_45_PENALTY=5.684541
PRIMER_LEFT_45_PENALTY=2.551554
PRIMER_RIGHT_45_PENALTY=3.132987
PRIMER_LEFT_45_SEQUENCE=AGCTGACCACCCATCAATAC
PRIMER_RIGHT_45_SEQUENCE=GCCGTACAAGTTCTCTCACAGTT
PRIMER_LEFT_45=621,20
PRIMER_RIGHT_45=1367,23
PRIMER_LEFT_45_TM=57.448
PRIMER_RIGHT_45_TM=59.867
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.826
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=5.6000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=747
PRIMER_PAIR_46_PENALTY=5.695516
PRIMER_LEFT_46_PENALTY=0.111534
PRIMER_RIGHT_46_PENALTY=5.583982
PRIMER_LEFT_46_SEQUENCE=CAACAAGCAACAAAAGCCAA
PRIMER_RIGHT_46_SEQUENCE=AGGTAGAATCCAACTCAAAACC
PRIMER_LEFT_46=177,20
PRIMER_RIGHT_46=1169,22
PRIMER_LEFT_46_TM=59.888
PRIMER_RIGHT_46_TM=56.416
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=10.0000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=993
PRIMER_PAIR_47_PENALTY=5.730975
PRIMER_LEFT_47_PENALTY=2.551554
PRIMER_RIGHT_47_PENALTY=3.179421
PRIMER_LEFT_47_SEQUENCE=AGCTGACCACCCATCAATAC
PRIMER_RIGHT_47_SEQUENCE=TATGCCGTACAAGTTCTCTCACA
PRIMER_LEFT_47=621,20
PRIMER_RIGHT_47=1370,23
PRIMER_LEFT_47_TM=57.448
PRIMER_RIGHT_47_TM=59.821
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=5.6000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=750
PRIMER_PAIR_48_PENALTY=5.789562
PRIMER_LEFT_48_PENALTY=1.390393
PRIMER_RIGHT_48_PENALTY=4.399169
PRIMER_LEFT_48_SEQUENCE=AGCTGACCACCCATCAATACA
PRIMER_RIGHT_48_SEQUENCE=TTATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_48=621,21
PRIMER_RIGHT_48=1371,22
PRIMER_LEFT_48_TM=60.390
PRIMER_RIGHT_48_TM=57.601
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=5.6000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=751
PRIMER_PAIR_49_PENALTY=5.789562
PRIMER_LEFT_49_PENALTY=1.390393
PRIMER_RIGHT_49_PENALTY=4.399169
PRIMER_LEFT_49_SEQUENCE=CAGCTGACCACCCATCAATAC
PRIMER_RIGHT_49_SEQUENCE=TTATGCCGTACAAGTTCTCTCA
PRIMER_LEFT_49=620,21
PRIMER_RIGHT_49=1371,22
PRIMER_LEFT_49_TM=60.390
PRIMER_RIGHT_49_TM=57.601
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=5.6000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=752
=
SEQUENCE_ID=MAGIC.338_4_4506041_4506421_zu-0_ler-0_14741_13601_TRUE
SEQUENCE_TARGET=950,480
SEQUENCE_TEMPLATE=ACAANCTTTNTTCCGAACTCANNNNNNNNNNNNNGAGTTGNAGGNTCAGTATCANNGGTATGANAANATCNAGGAGGAACTCTCGAATGCTCNNGNCAGACTNTNAGNNNNNNNNNNNNNNNNTTACGACTTGTCCAACCAGCTATCCNAANTGCANGCCANNTACAAAGCAATNGCCAAACNTCGAGATGNTNAGTTANNNNNNNNNNNNNNAAAAGCCAGGAAAGANGTAAANGGTCGCGGGATGGAANTGATTCAANGGGCNATCGTNTTNATCNAAACCGAGAAAACTAGGACGNANNTGGAATCAGANATCAAGGANTATGAAATCAACCTCCTCCTTCTGNGNCAAACCCATGACGAAGATTTTTCNGAAGNNCNNNNNAGGTCCGAATTGNAAGCNGTTNTAACAGAGAANCNAANTCNTCTGGCCGCTCNNCCNTCTTNNTCATTCAACCNTCAANNNNNNNNAGAGTTCTTGACCGAGTCGCCTCCTTTGAGCGAGTCNGGTTTAGATTNNNNNNNNNNNTTTTAAAACCTCAANTTNCGANNNNNNNNNNNNNNANNTTANACTTGCGTTTTCNTGATTCGAGATGATTNTCTCANGNNCCAGTGATCCCNTGGAACCNGAGGTATCNATCATGCCGNTNGANACTCCNANNNNGGTAGCCATCCCNNCAGAAATTCCTCCACCCACGAACACNGAAACAGTGGTNATCGAGGATNATGATGGTTCGNACNCTAGNATTCCNACNNNNNNNNNNNNNNNNNNNNNNNNNGATGAGACTGAGATGGCTGCCGTGGACCCGGTCAATCCTGAGCCAACCCAAACTNNNNANNTNNTGNNAAACTTGAANTTTATTTTTCAAACTANNTTCATCGCCTAAGTNGGNTTNNTATTTTCTGAACAAGTNNTTNNCCTGAGGAGGCTTTTAAACCTNTNNANATTATGGTCCCGAGANGGNNTTTTGCNNTTAANNTGTGGNTTTGCAAANTCCANNTTTTGGAAATAGNTTNNGTTAACCATGTGGNCAAGTTATGANATTCANAGGCTGATNAGACCTATCTCTTNAAANGTGAGTCTGAANTNGNTTTAANTNCGNGAATTCCCCAACTCGGGTTCNNTCAATTCGNCTTATNTNCTGGGCTTGGGNCTTANNNNNTTTTNTTNTATNTAATNAANNNTTTGTCTTTAATGCGACGGTTGCCATTTNNTTNCTNNGNAAATAGNAAAAGCNTCACNCCTNTNAGGCANTANTTNNNNNNNCCTCTATTTCCAAAAATGGTTTAAACCATTNGGACNCGCCANAATTATTANTTTTGTGGTTTNANNAANANCCCTAACTCNCTANANNTAAGAGCACAAACTNTNNNNNATGTNTCAAAANATGATCTCAGAAATTTACTTGGTAAANACTTCAAGNANGGAAAACGAANGANCCTCCACCNTNTGTCCTCTTGACCCNGNNAGCCCACTGNTTCTGTNCGGCGATGGCTTNGAGCCCGCCNNCGTNCTCTTTCCTCACNCNGNNTTTCACTTCCCNNCGAAGGANAANANNNNNNNNTACNATTTNATGGNAAANGTAGGTCTNTTACNCTTTTNCTTANCNNANNNNNTNCNNNNNNNNNNNNNNNNNNNNNNNNCTAGGTNNTCGACAAGCANCNCCGCCTATGCGATGNNNNNNNNNNNNNNNNNNNNNAGAGGNAAANGCTCNAGGCCCGAATTNCCCGNATNGAAAGGAAGGTGAGGGCCATNNAGAGNGATCCCTTCNAATNNAGGAACTTCGACTGTGTGGCGAAGATCCNGCACATGCTNCGGATGTANCTCNGTGCTCGAGGTNNAGTNNNNNNNNNNNNNNNNNNNNTGGTCCAAGTGATNCCGNCTGACNGNNAAGAGNNNGANGACGAGGANGANGACANCNACCCTTGGAAGNGNGTGAAGAATGAACCCAAAGTNGACNTCAANAACNAGGCATCNACCTCNAACCCNGAACCGGCGATGAANGAGCNTGTTCCCGAGCNNCTTCCCTAAATGTCCCNCNANTTCNNATNNNTNNNNNTTTTTTNNATTTTTGTTTGGACTTNNTNTTGGGNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNAAATATTCGTTTCCGAGATACTCTTTGAATGCAGGGNGATNNGGNNGACGTGAATGCTTCCGCNAATCACNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAAGTTACGAGTCNTTATAAATTGNATCNTNTATGTTTTCTTTATTCNTCATCTAACTTTNGTTTTTCCNTTTNTGNAGTTATGGCTTTTAACCNTCGNNNNNNNNNNNNNATGCCANACTTTTNCANNNNNNNGATGA
PRIMER_LEFT_NUM_RETURNED=33
PRIMER_RIGHT_NUM_RETURNED=33
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=33
PRIMER_PAIR_0_PENALTY=1.189287
PRIMER_LEFT_0_PENALTY=0.882618
PRIMER_RIGHT_0_PENALTY=0.306669
PRIMER_LEFT_0_SEQUENCE=ATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_0_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_0=813,20
PRIMER_RIGHT_0=1796,20
PRIMER_LEFT_0_TM=60.883
PRIMER_RIGHT_0_TM=60.307
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=984
PRIMER_PAIR_1_PENALTY=1.555886
PRIMER_LEFT_1_PENALTY=1.249217
PRIMER_RIGHT_1_PENALTY=0.306669
PRIMER_LEFT_1_SEQUENCE=AATCCTGAGCCAACCCAAAC
PRIMER_RIGHT_1_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_1=812,20
PRIMER_RIGHT_1=1796,20
PRIMER_LEFT_1_TM=61.249
PRIMER_RIGHT_1_TM=60.307
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=985
PRIMER_PAIR_2_PENALTY=3.410245
PRIMER_LEFT_2_PENALTY=3.103576
PRIMER_RIGHT_2_PENALTY=0.306669
PRIMER_LEFT_2_SEQUENCE=AATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_2_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_2=812,21
PRIMER_RIGHT_2=1796,20
PRIMER_LEFT_2_TM=62.104
PRIMER_RIGHT_2_TM=60.307
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=985
PRIMER_PAIR_3_PENALTY=3.502503
PRIMER_LEFT_3_PENALTY=0.882618
PRIMER_RIGHT_3_PENALTY=2.619885
PRIMER_LEFT_3_SEQUENCE=ATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_3_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_3=813,20
PRIMER_RIGHT_3=1754,20
PRIMER_LEFT_3_TM=60.883
PRIMER_RIGHT_3_TM=62.620
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=942
PRIMER_PAIR_4_PENALTY=3.536379
PRIMER_LEFT_4_PENALTY=3.229711
PRIMER_RIGHT_4_PENALTY=0.306669
PRIMER_LEFT_4_SEQUENCE=CAATCCTGAGCCAACCCAAA
PRIMER_RIGHT_4_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_4=811,20
PRIMER_RIGHT_4=1796,20
PRIMER_LEFT_4_TM=63.230
PRIMER_RIGHT_4_TM=60.307
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=986
PRIMER_PAIR_5_PENALTY=3.672489
PRIMER_LEFT_5_PENALTY=3.365820
PRIMER_RIGHT_5_PENALTY=0.306669
PRIMER_LEFT_5_SEQUENCE=GTCAATCCTGAGCCAACCCA
PRIMER_RIGHT_5_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_5=809,20
PRIMER_RIGHT_5=1796,20
PRIMER_LEFT_5_TM=63.366
PRIMER_RIGHT_5_TM=60.307
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=988
PRIMER_PAIR_6_PENALTY=3.869102
PRIMER_LEFT_6_PENALTY=1.249217
PRIMER_RIGHT_6_PENALTY=2.619885
PRIMER_LEFT_6_SEQUENCE=AATCCTGAGCCAACCCAAAC
PRIMER_RIGHT_6_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_6=812,20
PRIMER_RIGHT_6=1754,20
PRIMER_LEFT_6_TM=61.249
PRIMER_RIGHT_6_TM=62.620
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=943
PRIMER_PAIR_7_PENALTY=4.142425
PRIMER_LEFT_7_PENALTY=3.835757
PRIMER_RIGHT_7_PENALTY=0.306669
PRIMER_LEFT_7_SEQUENCE=TCAATCCTGAGCCAACCCAA
PRIMER_RIGHT_7_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_7=810,20
PRIMER_RIGHT_7=1796,20
PRIMER_LEFT_7_TM=63.836
PRIMER_RIGHT_7_TM=60.307
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=10.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=987
PRIMER_PAIR_8_PENALTY=4.349634
PRIMER_LEFT_8_PENALTY=4.042965
PRIMER_RIGHT_8_PENALTY=0.306669
PRIMER_LEFT_8_SEQUENCE=GGTCAATCCTGAGCCAACCC
PRIMER_RIGHT_8_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_8=808,20
PRIMER_RIGHT_8=1796,20
PRIMER_LEFT_8_TM=64.043
PRIMER_RIGHT_8_TM=60.307
PRIMER_LEFT_8_GC_PERCENT=60.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=989
PRIMER_PAIR_9_PENALTY=5.211465
PRIMER_LEFT_9_PENALTY=4.904797
PRIMER_RIGHT_9_PENALTY=0.306669
PRIMER_LEFT_9_SEQUENCE=CAATCCTGAGCCAACCCAAAC
PRIMER_RIGHT_9_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_9=811,21
PRIMER_RIGHT_9=1796,20
PRIMER_LEFT_9_TM=63.905
PRIMER_RIGHT_9_TM=60.307
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=986
PRIMER_PAIR_10_PENALTY=5.723461
PRIMER_LEFT_10_PENALTY=3.103576
PRIMER_RIGHT_10_PENALTY=2.619885
PRIMER_LEFT_10_SEQUENCE=AATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_10_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_10=812,21
PRIMER_RIGHT_10=1754,20
PRIMER_LEFT_10_TM=62.104
PRIMER_RIGHT_10_TM=62.620
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=943
PRIMER_PAIR_11_PENALTY=5.809867
PRIMER_LEFT_11_PENALTY=5.503198
PRIMER_RIGHT_11_PENALTY=0.306669
PRIMER_LEFT_11_SEQUENCE=GTCAATCCTGAGCCAACCCAA
PRIMER_RIGHT_11_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_11=809,21
PRIMER_RIGHT_11=1796,20
PRIMER_LEFT_11_TM=64.503
PRIMER_RIGHT_11_TM=60.307
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=10.0000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=988
PRIMER_PAIR_12_PENALTY=5.849595
PRIMER_LEFT_12_PENALTY=3.229711
PRIMER_RIGHT_12_PENALTY=2.619885
PRIMER_LEFT_12_SEQUENCE=CAATCCTGAGCCAACCCAAA
PRIMER_RIGHT_12_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_12=811,20
PRIMER_RIGHT_12=1754,20
PRIMER_LEFT_12_TM=63.230
PRIMER_RIGHT_12_TM=62.620
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.8000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=944
PRIMER_PAIR_13_PENALTY=5.985705
PRIMER_LEFT_13_PENALTY=3.365820
PRIMER_RIGHT_13_PENALTY=2.619885
PRIMER_LEFT_13_SEQUENCE=GTCAATCCTGAGCCAACCCA
PRIMER_RIGHT_13_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_13=809,20
PRIMER_RIGHT_13=1754,20
PRIMER_LEFT_13_TM=63.366
PRIMER_RIGHT_13_TM=62.620
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=946
PRIMER_PAIR_14_PENALTY=6.237895
PRIMER_LEFT_14_PENALTY=5.931227
PRIMER_RIGHT_14_PENALTY=0.306669
PRIMER_LEFT_14_SEQUENCE=TCAATCCTGAGCCAACCCAAA
PRIMER_RIGHT_14_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_14=810,21
PRIMER_RIGHT_14=1796,20
PRIMER_LEFT_14_TM=64.931
PRIMER_RIGHT_14_TM=60.307
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.8000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=987
PRIMER_PAIR_15_PENALTY=6.455641
PRIMER_LEFT_15_PENALTY=3.835757
PRIMER_RIGHT_15_PENALTY=2.619885
PRIMER_LEFT_15_SEQUENCE=TCAATCCTGAGCCAACCCAA
PRIMER_RIGHT_15_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_15=810,20
PRIMER_RIGHT_15=1754,20
PRIMER_LEFT_15_TM=63.836
PRIMER_RIGHT_15_TM=62.620
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.0000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=945
PRIMER_PAIR_16_PENALTY=6.662850
PRIMER_LEFT_16_PENALTY=4.042965
PRIMER_RIGHT_16_PENALTY=2.619885
PRIMER_LEFT_16_SEQUENCE=GGTCAATCCTGAGCCAACCC
PRIMER_RIGHT_16_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_16=808,20
PRIMER_RIGHT_16=1754,20
PRIMER_LEFT_16_TM=64.043
PRIMER_RIGHT_16_TM=62.620
PRIMER_LEFT_16_GC_PERCENT=60.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=947
PRIMER_PAIR_17_PENALTY=6.788643
PRIMER_LEFT_17_PENALTY=0.882618
PRIMER_RIGHT_17_PENALTY=5.906025
PRIMER_LEFT_17_SEQUENCE=ATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_17_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_17=813,20
PRIMER_RIGHT_17=1797,21
PRIMER_LEFT_17_TM=60.883
PRIMER_RIGHT_17_TM=64.906
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=57.143
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=985
PRIMER_PAIR_18_PENALTY=6.926500
PRIMER_LEFT_18_PENALTY=6.619831
PRIMER_RIGHT_18_PENALTY=0.306669
PRIMER_LEFT_18_SEQUENCE=CAATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_18_SEQUENCE=GCCACACAGTCGAAGTTCCT
PRIMER_LEFT_18=811,22
PRIMER_RIGHT_18=1796,20
PRIMER_LEFT_18_TM=64.620
PRIMER_RIGHT_18_TM=60.307
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=986
PRIMER_PAIR_19_PENALTY=7.155242
PRIMER_LEFT_19_PENALTY=1.249217
PRIMER_RIGHT_19_PENALTY=5.906025
PRIMER_LEFT_19_SEQUENCE=AATCCTGAGCCAACCCAAAC
PRIMER_RIGHT_19_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_19=812,20
PRIMER_RIGHT_19=1797,21
PRIMER_LEFT_19_TM=61.249
PRIMER_RIGHT_19_TM=64.906
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=57.143
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=986
PRIMER_PAIR_20_PENALTY=7.524682
PRIMER_LEFT_20_PENALTY=4.904797
PRIMER_RIGHT_20_PENALTY=2.619885
PRIMER_LEFT_20_SEQUENCE=CAATCCTGAGCCAACCCAAAC
PRIMER_RIGHT_20_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_20=811,21
PRIMER_RIGHT_20=1754,20
PRIMER_LEFT_20_TM=63.905
PRIMER_RIGHT_20_TM=62.620
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=944
PRIMER_PAIR_21_PENALTY=8.123083
PRIMER_LEFT_21_PENALTY=5.503198
PRIMER_RIGHT_21_PENALTY=2.619885
PRIMER_LEFT_21_SEQUENCE=GTCAATCCTGAGCCAACCCAA
PRIMER_RIGHT_21_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_21=809,21
PRIMER_RIGHT_21=1754,20
PRIMER_LEFT_21_TM=64.503
PRIMER_RIGHT_21_TM=62.620
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=10.0000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=946
PRIMER_PAIR_22_PENALTY=8.551111
PRIMER_LEFT_22_PENALTY=5.931227
PRIMER_RIGHT_22_PENALTY=2.619885
PRIMER_LEFT_22_SEQUENCE=TCAATCCTGAGCCAACCCAAA
PRIMER_RIGHT_22_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_22=810,21
PRIMER_RIGHT_22=1754,20
PRIMER_LEFT_22_TM=64.931
PRIMER_RIGHT_22_TM=62.620
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.8000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=945
PRIMER_PAIR_23_PENALTY=9.009602
PRIMER_LEFT_23_PENALTY=3.103576
PRIMER_RIGHT_23_PENALTY=5.906025
PRIMER_LEFT_23_SEQUENCE=AATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_23_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_23=812,21
PRIMER_RIGHT_23=1797,21
PRIMER_LEFT_23_TM=62.104
PRIMER_RIGHT_23_TM=64.906
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=57.143
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=986
PRIMER_PAIR_24_PENALTY=9.135736
PRIMER_LEFT_24_PENALTY=3.229711
PRIMER_RIGHT_24_PENALTY=5.906025
PRIMER_LEFT_24_SEQUENCE=CAATCCTGAGCCAACCCAAA
PRIMER_RIGHT_24_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_24=811,20
PRIMER_RIGHT_24=1797,21
PRIMER_LEFT_24_TM=63.230
PRIMER_RIGHT_24_TM=64.906
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=57.143
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=987
PRIMER_PAIR_25_PENALTY=9.239716
PRIMER_LEFT_25_PENALTY=6.619831
PRIMER_RIGHT_25_PENALTY=2.619885
PRIMER_LEFT_25_SEQUENCE=CAATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_25_SEQUENCE=ATGGCCCTCACCTTCCTTTC
PRIMER_LEFT_25=811,22
PRIMER_RIGHT_25=1754,20
PRIMER_LEFT_25_TM=64.620
PRIMER_RIGHT_25_TM=62.620
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=944
PRIMER_PAIR_26_PENALTY=9.271845
PRIMER_LEFT_26_PENALTY=3.365820
PRIMER_RIGHT_26_PENALTY=5.906025
PRIMER_LEFT_26_SEQUENCE=GTCAATCCTGAGCCAACCCA
PRIMER_RIGHT_26_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_26=809,20
PRIMER_RIGHT_26=1797,21
PRIMER_LEFT_26_TM=63.366
PRIMER_RIGHT_26_TM=64.906
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=57.143
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=989
PRIMER_PAIR_27_PENALTY=9.741782
PRIMER_LEFT_27_PENALTY=3.835757
PRIMER_RIGHT_27_PENALTY=5.906025
PRIMER_LEFT_27_SEQUENCE=TCAATCCTGAGCCAACCCAA
PRIMER_RIGHT_27_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_27=810,20
PRIMER_RIGHT_27=1797,21
PRIMER_LEFT_27_TM=63.836
PRIMER_RIGHT_27_TM=64.906
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=57.143
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=10.0000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=988
PRIMER_PAIR_28_PENALTY=9.948991
PRIMER_LEFT_28_PENALTY=4.042965
PRIMER_RIGHT_28_PENALTY=5.906025
PRIMER_LEFT_28_SEQUENCE=GGTCAATCCTGAGCCAACCC
PRIMER_RIGHT_28_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_28=808,20
PRIMER_RIGHT_28=1797,21
PRIMER_LEFT_28_TM=64.043
PRIMER_RIGHT_28_TM=64.906
PRIMER_LEFT_28_GC_PERCENT=60.000
PRIMER_RIGHT_28_GC_PERCENT=57.143
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=990
PRIMER_PAIR_29_PENALTY=10.810822
PRIMER_LEFT_29_PENALTY=4.904797
PRIMER_RIGHT_29_PENALTY=5.906025
PRIMER_LEFT_29_SEQUENCE=CAATCCTGAGCCAACCCAAAC
PRIMER_RIGHT_29_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_29=811,21
PRIMER_RIGHT_29=1797,21
PRIMER_LEFT_29_TM=63.905
PRIMER_RIGHT_29_TM=64.906
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=57.143
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=987
PRIMER_PAIR_30_PENALTY=11.409224
PRIMER_LEFT_30_PENALTY=5.503198
PRIMER_RIGHT_30_PENALTY=5.906025
PRIMER_LEFT_30_SEQUENCE=GTCAATCCTGAGCCAACCCAA
PRIMER_RIGHT_30_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_30=809,21
PRIMER_RIGHT_30=1797,21
PRIMER_LEFT_30_TM=64.503
PRIMER_RIGHT_30_TM=64.906
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=57.143
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=10.0000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=989
PRIMER_PAIR_31_PENALTY=11.837252
PRIMER_LEFT_31_PENALTY=5.931227
PRIMER_RIGHT_31_PENALTY=5.906025
PRIMER_LEFT_31_SEQUENCE=TCAATCCTGAGCCAACCCAAA
PRIMER_RIGHT_31_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_31=810,21
PRIMER_RIGHT_31=1797,21
PRIMER_LEFT_31_TM=64.931
PRIMER_RIGHT_31_TM=64.906
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=57.143
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.8000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=988
PRIMER_PAIR_32_PENALTY=12.525856
PRIMER_LEFT_32_PENALTY=6.619831
PRIMER_RIGHT_32_PENALTY=5.906025
PRIMER_LEFT_32_SEQUENCE=CAATCCTGAGCCAACCCAAACT
PRIMER_RIGHT_32_SEQUENCE=CGCCACACAGTCGAAGTTCCT
PRIMER_LEFT_32=811,22
PRIMER_RIGHT_32=1797,21
PRIMER_LEFT_32_TM=64.620
PRIMER_RIGHT_32_TM=64.906
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=57.143
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=987
=
SEQUENCE_ID=MAGIC.175_4_7369201_7369867_can-0_wu-0_552947_1280_TRUE
SEQUENCE_TARGET=950,766
SEQUENCE_TEMPLATE=CGNNNNAANTTAAACAAAAACTCGTCTATACAGTTATATTTTTATTAGNATGAANCAAAAAAANANTGGAATTTTAGGTGTAAGGACAAAATTGCGTGCGTTTNAATTTCCAAATGACGATANTTTTCAAAACTGNGAAAATGTTTNTTTTTGCAGCAGAATATAATATTNTAACGTACGAGGAAACNAAACGGAACTAAAAATACAGAGAACGAGAAAATATTCAATGTTAAATTAGAAGANGAAAAAACTGANCTAGATCCAAAAATTCTCAGAAATGAATTATTTATAAAGTTTAAGCAAAAACGTTGAAATTNATTAATNCAAACCTGATTCAAAAGAGCTCCAGAAGGAAGGNAAAAAANNCTTCGGAAAGATGAAGNTTTTTGTTTCCTTTGATNGAAGAAATTGAATCTCAGANATCGCAGAAGAAACNAAATCCAAAAATGAGATTTGANGGNTTTNTTTTGATTAAAATTGAAAACCCTAANAAAAAGAGAGAGTCGAGAATGTAGGAGGAAGAGAANAAAGGGGAGAAGAAAATTGCGGAGGGTTTTTCGTATTTATANACTCTCNNTNTCTTTTAGAGACTTTGAGATAAATTAGAGACAGAGACGTGGTTTANAANAACCATCCATGTTGGGNTTATTTACCAAAATTTNCCATCNTTNTAAAATGATNGACCAATCAATAACACGTTATGAGNNTGTCATTTAGTCATACGCGATTTTCAGAGATTTCGNAAAACGATTTTTGGTTCGATTACAAATTATGGCGGTTTGTTTATTTCACTTTTAGTNAGGTCGGTCAAANATTNTTATTATNAAGATCTTTTTNTTGTANANAAGANNNATTTTATNAGAGATGANATTAATTAAGTTATTCGTTATNCNNGNATATATAAATTCACAAACGTTTTGCNTTNNNTNANAAAGATANGTTTGNAATNATTTNTNTTGNTTTAAACTCTAGAACANACATAGATATTNNTATNTTCTTANTTATTTCTTTCAAGTGANTTATTGGTATGGCGTGGAATCTTGNTGTAACCTATGTTTGAANATAAACTGTATAAAACCTGTGTGCCTTTGAAGTTAATTCACGTAATCAAATTACTACTATTTCTTTCTCAATTTGGANCACGTAAGTTCTCGAGAATCCATTTANTNAATANACCACATTTATTTTTCCNAACTTTGTTANGNCAAAANNNTTTTNNNTTTNTTTNNNNNTAAGATNGTTTTNNAGTATTATAGTANTTGTNAGNAGNCANNAATTAAATTNTAAGTTANGNNNTATATANTTTTGAAATGAAAAAGNTTNTTTTTTTTCCTTAATTANATGTGAGAAGTGTTAATNGTATTACAACGGTAGGACGTTGGTTGTTAAATAAGCTGGTTCGTTAACATACACGTGGCAATGAGTNAAAAGAGACTAAAAGCTCGTAGACGACACGTGTTGAANTGTAATGTTGACGCGTCCACNTGTTATTTGGGCCTTAGATGGGCCCTTCTTGCTAAAAAAGGCAACAAAANATCTGTCTTNTCTTTTTTGTCTTTCTCTTTCGCATTATCTTCACTTTACAACCTTCGCGATATANTTCATTATCNTNTAATTTAATATNTTTTNCACTANTATNANNNTTNTNTNTATAATATAGTAATNTNNTTNNAAANTNGAANTNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAANAATTGTGTATAGTGNGCTTTTGGGCCGCGGGGGTCTTGNGTGTGTAGAGAAAAGGTCACNTCACGTGGATCATGGAAGGGTTTAAAGTGNAAATATCGGTTAAATCACAAGTTTTTCTAGTTGGGAATTGACATTAAAGTTAACGGTCATAAACGGTGTTAGTTAAAAAAAGTGTTAGAAAATCATGTTTGNTTCACAAAAANNANCNAAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNCGTGCATCATTGAATCTGTACTTTATGATGAGTCACGAAGACATAAGTCTCAGATCTGACTAAGAATCTTTTTTCGAGTTTCCCTGAAATGTATCCATTGAAACTGTCTACTNNNTTTTTTTTCGTGTCCANTATACAAANTTTTCTCTATCGTGTGCTTTCAAANATTCGTTAATATGTNTTTTTGCTTATNTNGAGNCANTATACATNNTTTTTTTGTATAAAATGATATTAGTANTATTCCTAACTAAAGAATAAACAATAATGTCAAGAATTGCATCATTATGTAGTATTTTACTATGNNATAATNNNANNATTGTTTGAACCATGCATGATTTGACACTTNTTATAATTCCATTCAAANAAGCAAAATTAAACAATCTCCGGAAGTTGAAAATGTGTGGTGTTAATTNAACTAAATATACAATGAATCTANNTTTTTNATTGTGTTTTNTATNATTTTAAATNGTACNNANAATAAANTAATNTATAANGAATTATATACCANAAGAAAGACGGTGCNTACCAAACTCATTAGTGATTNTATTATTGGTGGGTTTTAGNNNNANNNNNNNTTAATAGTCTTATNAAAAAANNNNNNNNNNNNNNNNNNNNNNGTATACGACACACGAGG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4293652_4293793_mt-0_wil-2_33346_22549_TRUE
SEQUENCE_TARGET=950,241
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTNGATCTTGATGGATGTATCTNCNATGCTTCCTATTTTTATATTTATGTATGTCNTTTTATTAGGTTAAACTTTGTTTTGAAACTTANGGTNTTTTGTNTATNTTTGTCTAAGATTCANACTACTNTTTATGATTCAGTGTATTNTTTAAGGCCTCTTGCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14026382_14026769_rsch-4_kn-0_48792_11252_TRUE
SEQUENCE_TARGET=950,487
SEQUENCE_TEMPLATE=TGAGAAATCCCTGTCCGTGACCGAGAATCATTGAAGCTAACCTTAAACCTTCNTCNGAATCTCTGTGAGTCTCNAGCGTGTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTNTGNAACATCAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTGTCGAGTTTCAAAGTGACCGTCCGACCGAGAAATCGTGAAAAGTGTTACNATCGAGAAACTATGCCCGTGATCAAGATCCTTTNTNNGTANCTTATTATTTCCTGTCCGTGATNAAGANAACCTGTTTGTGGATGAGAAATCCCTGNCCGTGACCGAGAATCATTGAAGCTAACCTTAAACCTTNATCNGAATCTCTGTGAGTCTCNAGCGTGTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_10192929_10193405_no-0_po-0_3573_329669_TRUE
SEQUENCE_TARGET=950,576
SEQUENCE_TEMPLATE=GATNTCAACATCGATTTCTCACAAGTTGAATGAAGNGTGTGACACGNNNAAAAATNGTTCATAAGGAAGAAATCGATGAGATAACTTGGNAAATTGGNATGTGTACTNCTCAATCCTTCATTCTTCGGAAANANTTATACTACTTAATTCCTATCACAATGCATTNATGCTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANACTATAAACGTTGCTTCACACGGTATGTATCAGCGGNANGCANATATAACGCACCTGTGCNACACCGAGACANTGACAGTCCATAGCTGCAATTGCCACCTGCTCATTTAGTGTCCATTCTNNACAANAAAGAGNAACAAGANCNATTTANNTACATTNATAAACATAAGAGCAGAANAATATCTTAATTTANTCTTGATTATAATTGCATANTTTACACAAAAATCNATAGCTAAAATGAACCAAAACAAAAACATTTCATNGAGNCAAGAAAGTTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTACTAATCATTCAATTTTNNCTAAAGNTTTTTTTGACTAAAGATTAATACAAACTATAGCACNANAAATGATCATTACTGAATTGCTTTACAGATCCANTTAGACNANTNATTATCCTAATTTAATTCAGACATTGATCACTTAANNAANTTCCTTAAGAACACATGNTTAATTTCACCGGTCGGATCAATGAATATTAAACCAGAGATGAATCAGNGATTCGAAATTCGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACCGTGTTTTAANACTTTCTCCGACCGNCGAANTTTGAGATTACGAANGAGNCAAAGATACTCCCATGTTCCNCCTCCGCCATTATCGACTTGANTCTCTAGCTGGTTCAATNGCNTCTCCTCTGTTTTCGTCACCATCTCTCNCGCTCTTGTGATTNTGCTTGAACTTACGAGCATTGTGGATCTGTTTGATTGAACGACGACGNAGAGAACATNGNNCNGGTTCGGTTTATGACTGTACCGATCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14007681_14007864_wil-2_wu-0_29729_6575_TRUE
SEQUENCE_TARGET=950,283
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4715618_4715985_ler-0_edi-0_3015_3160_TRUE
SEQUENCE_TARGET=950,467
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCCGCATCGAGTACCAACGTAGTCCTTNNNNNNNNNNNGAGNTTTGCTAGTAAAATTCTNGGCTCNNACTTTAAATTGAGTCAATNCCATCAAGAAGAACTCAATGACCCAAATACATATAATCGCATANAGATAATTGAATAATCTTGGCAAGATAAATCNTCTTGCTTATTATCATCTNTCTCTACCAATATAACCAATTAAGATTCTTTAAAGNCTTTATTTTAGCATGGGTGGCTTATGANTAATACTCAACCATCTANTTTCTTCTNCCACAANTAATCATNCAACAAGCTAAAACATATATCANTGACATGGCAGTATCTTAAGGACNNCNATTACAAAGACATCTAATTCTATAAGATCATTCAGAGAATTGGGAAAANCCATAAAGAAATGACGAANGTAAAGTTATCTACTAAAGCATATAAANTATNGTTTATAAATCACAAACATATNGAAGCTANTTATGAGGCCATATTAAGGTGAAAACAAANNNNNAATNTAGGCNACAAAGACTACTGAGNTTTTCACTAACTNACAAACCAATTCACACTCCAACTTAANGTGATTCAACCAAAGACATATCATGACTNGTCGACNAACAAGCATGTCTAATGCANCTTAACAGACGAACAAGTGATATGGTTAGAAATTTGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATANATGAATCTGAANNGAGTTGAAGATGANGCNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_10868929_10869466_bur-0_oy-0_71247_641264_TRUE
SEQUENCE_TARGET=950,637
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_6764834_6764875_ct-1_po-0_8394_94995_TRUE
SEQUENCE_TARGET=950,141
SEQUENCE_TEMPLATE=NNNGTCAGTCTCCAACGCCGNCAAANTTTNATCTTCTTCCTCTTNATAGNTTTTCNACTNTCCGTTTAGTGTTTTTTNTTTTGTTTTTGAGAATGTGATTAAGNNNNNNNNNNAAAGGCGTTNNACTCTTTCGTATCCTAANGAAGNGTGTGTCACCGAAAGCGTATAATCNNATANTAAATCCGATCATTAGGNNTGCGAAATTCGGATTNGTTNGAATNAACNAAACCTAAGATTCGNNGNTTNTTTNGTCGCCTTCNNNNNNNNNAAANTTCGANTNTTATTGAGAAATNNATGGGNTTATAATTGGNATTGCTTCATAATGGTGAANNCTCTTNNATATNAAAAAAGTGAGAGATGTTCAAGTTTNCNGAGCAAATATTAAACTGGCCAGAATTTAACAGCTCTGTGTTTAATTTTACTATTTTCACAGAGTTACCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNATGGATANTTTCTGATTATTGTTTNCCAANTGNNNNNNNNGCCCACCAAACCAACTTACTAAACCGGATTGGATCACCATTTTATTGACCATNAAACTAAACNAAACCAANTCCAGAAAAATCAGNTTTTACATAAATTGATCCATAAGNATGGNTACGTGAACCGAACCGGTTTTCAATTCATNCAAGGTGATCTATCCGGTTCATTAGAAACTNTANNTGTTAACCAAATCTCTTCANNTTTACCAAAATTTACATGACCTATATACNAAACCTCNGTTTTCAATTCATNCAGTTTTTAAGCCATTGACTACATNCAAGNAAACTGGTTATTTACCGGTTCGNCCATTCANTATGATCTCCAANANGACATCGTTCAAGTGATCAATNGGACCAGGCAAGCACCAATGCAGACAATCGTTCTGTACCGTAGCGTNCTTATCNTTATCAAACGGCCTANACTCTCNATANGGACCNGGATGNCCATCNGGTCGAGTCAGCAACATNCCAGCAAAATCNAGAAGCTTTAGATTCTCACTNTCCTGACCCATCTCNGTAANCACTCTNTCGAATTGNTTAATCTCAACATCTCTCAATATCTTGTTCAGAACTTTCATCTCGACNNCCTCTTCACNCACCGGTTCTGTTTTCTTACAAGTCCCNCCGTTATGCCATTCCCCATCTTCAAANTGATCAGGTATCGAAGTCCGGAAAAAGATCATGCCTTTAGTNTTAGATTTCGCTATGAAGTCCATAACNTGNCGCANCGACGTATTGTAAGCATAGTCNAATCCTAAATCCGTCATGTTCNAACTNNCCGGGCAGCCATGGCATCCNACGGGATTAGNATTCTCGTGGTATACCGCAGTTTTCAAGAACCATTCNCCTGAAGAGATGANCGCATAGTCAAGGCTNGGGAATAGATCAGTCCATGTATTGTCGAGTTTATCTAGATGAAGCTGNACTGCAGCTGATGAGACACCATTTGAGTCTTCAAAGATATCNGCTTGAACAAGAAAAGGNGACCAAATGTTGGATACCGTAAAGTTNTNNNANGGNAAATGCCAACGCTTCGATCTATAGTTCTCATCGTGATACACTTCNACNGGTTTTTCAACCTGAAACATNGNNANNNNANNNNNNNNNNACAATCACAANTCTNATTNTNNTCAAGNNTGCAAATAGCTAGCAAGCAATAGCTAAAAACCTTACTGTAGATAGCATACAAAGCAAAGACTCGACATGGTTACGAGCAATGGAGTCACCGATTATAGCCCAAGATNTATTCCTCATAAGCTGCAGAAATCTCNGAGAATCAAACCGAGGCAAGGAACAGTCATTAGGCTTCCATTTCCANTNGAGAAAACCAGAATCAGGTCGACCATTAGTGATGCAATTCTGGTGAGCATCTACAACGATACCACATGACTCNTTCGTGTAGATTGGTCCCAAAGGATCTTTAATCCATTTCCCAGTAAATAGATCACACTTCTCCACCTCAATATCTAATNCAACAAAACAGATTCTTATCATCACAAACTAACAGAANCTAATGAACC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.821864
PRIMER_LEFT_0_PENALTY=0.755541
PRIMER_RIGHT_0_PENALTY=0.066323
PRIMER_LEFT_0_SEQUENCE=CTAAACCGGATTGGATCACC
PRIMER_RIGHT_0_SEQUENCE=TTTCCGGACTTCGATACCTG
PRIMER_LEFT_0=540,20
PRIMER_RIGHT_0=1195,20
PRIMER_LEFT_0_TM=59.244
PRIMER_RIGHT_0_TM=60.066
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=656
PRIMER_PAIR_1_PENALTY=1.033140
PRIMER_LEFT_1_PENALTY=0.755541
PRIMER_RIGHT_1_PENALTY=0.277599
PRIMER_LEFT_1_SEQUENCE=CTAAACCGGATTGGATCACC
PRIMER_RIGHT_1_SEQUENCE=GAAGATGGGGAATGGCATAA
PRIMER_LEFT_1=540,20
PRIMER_RIGHT_1=1167,20
PRIMER_LEFT_1_TM=59.244
PRIMER_RIGHT_1_TM=59.722
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=628
PRIMER_PAIR_2_PENALTY=1.107937
PRIMER_LEFT_2_PENALTY=1.041614
PRIMER_RIGHT_2_PENALTY=0.066323
PRIMER_LEFT_2_SEQUENCE=GCCCACCAAACCAACTTACT
PRIMER_RIGHT_2_SEQUENCE=TTTCCGGACTTCGATACCTG
PRIMER_LEFT_2=522,20
PRIMER_RIGHT_2=1195,20
PRIMER_LEFT_2_TM=58.958
PRIMER_RIGHT_2_TM=60.066
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=674
PRIMER_PAIR_3_PENALTY=1.133676
PRIMER_LEFT_3_PENALTY=1.067353
PRIMER_RIGHT_3_PENALTY=0.066323
PRIMER_LEFT_3_SEQUENCE=ACTAAACCGGATTGGATCACC
PRIMER_RIGHT_3_SEQUENCE=TTTCCGGACTTCGATACCTG
PRIMER_LEFT_3=539,21
PRIMER_RIGHT_3=1195,20
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=60.066
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=657
PRIMER_PAIR_4_PENALTY=1.319213
PRIMER_LEFT_4_PENALTY=1.041614
PRIMER_RIGHT_4_PENALTY=0.277599
PRIMER_LEFT_4_SEQUENCE=GCCCACCAAACCAACTTACT
PRIMER_RIGHT_4_SEQUENCE=GAAGATGGGGAATGGCATAA
PRIMER_LEFT_4=522,20
PRIMER_RIGHT_4=1167,20
PRIMER_LEFT_4_TM=58.958
PRIMER_RIGHT_4_TM=59.722
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=646
PRIMER_PAIR_5_PENALTY=1.344952
PRIMER_LEFT_5_PENALTY=1.067353
PRIMER_RIGHT_5_PENALTY=0.277599
PRIMER_LEFT_5_SEQUENCE=ACTAAACCGGATTGGATCACC
PRIMER_RIGHT_5_SEQUENCE=GAAGATGGGGAATGGCATAA
PRIMER_LEFT_5=539,21
PRIMER_RIGHT_5=1167,20
PRIMER_LEFT_5_TM=60.067
PRIMER_RIGHT_5_TM=59.722
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=629
PRIMER_PAIR_6_PENALTY=1.404877
PRIMER_LEFT_6_PENALTY=1.338553
PRIMER_RIGHT_6_PENALTY=0.066323
PRIMER_LEFT_6_SEQUENCE=ACCGGATTGGATCACCATTT
PRIMER_RIGHT_6_SEQUENCE=TTTCCGGACTTCGATACCTG
PRIMER_LEFT_6=544,20
PRIMER_RIGHT_6=1195,20
PRIMER_LEFT_6_TM=61.339
PRIMER_RIGHT_6_TM=60.066
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.2000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=652
PRIMER_PAIR_7_PENALTY=1.616152
PRIMER_LEFT_7_PENALTY=1.338553
PRIMER_RIGHT_7_PENALTY=0.277599
PRIMER_LEFT_7_SEQUENCE=ACCGGATTGGATCACCATTT
PRIMER_RIGHT_7_SEQUENCE=GAAGATGGGGAATGGCATAA
PRIMER_LEFT_7=544,20
PRIMER_RIGHT_7=1167,20
PRIMER_LEFT_7_TM=61.339
PRIMER_RIGHT_7_TM=59.722
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.2000
PRIMER_RIGHT_7_END_STABILITY=6.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=624
PRIMER_PAIR_8_PENALTY=1.803193
PRIMER_LEFT_8_PENALTY=0.755541
PRIMER_RIGHT_8_PENALTY=1.047652
PRIMER_LEFT_8_SEQUENCE=CTAAACCGGATTGGATCACC
PRIMER_RIGHT_8_SEQUENCE=TTTTTCCGGACTTCGATACCT
PRIMER_LEFT_8=540,20
PRIMER_RIGHT_8=1197,21
PRIMER_LEFT_8_TM=59.244
PRIMER_RIGHT_8_TM=59.952
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=658
PRIMER_PAIR_9_PENALTY=1.885896
PRIMER_LEFT_9_PENALTY=1.819573
PRIMER_RIGHT_9_PENALTY=0.066323
PRIMER_LEFT_9_SEQUENCE=CCGGATTGGATCACCATTTT
PRIMER_RIGHT_9_SEQUENCE=TTTCCGGACTTCGATACCTG
PRIMER_LEFT_9=545,20
PRIMER_RIGHT_9=1195,20
PRIMER_LEFT_9_TM=61.820
PRIMER_RIGHT_9_TM=60.066
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=651
PRIMER_PAIR_10_PENALTY=1.955457
PRIMER_LEFT_10_PENALTY=1.273836
PRIMER_RIGHT_10_PENALTY=0.681621
PRIMER_LEFT_10_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_10_SEQUENCE=TGCTCGTAACCATGTCGAGT
PRIMER_LEFT_10=881,20
PRIMER_RIGHT_10=1736,20
PRIMER_LEFT_10_TM=58.726
PRIMER_RIGHT_10_TM=59.318
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=856
PRIMER_PAIR_11_PENALTY=2.015491
PRIMER_LEFT_11_PENALTY=1.273836
PRIMER_RIGHT_11_PENALTY=0.741655
PRIMER_LEFT_11_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_11_SEQUENCE=TAGATCGAAGCGTTGGCATT
PRIMER_LEFT_11=881,20
PRIMER_RIGHT_11=1561,20
PRIMER_LEFT_11_TM=58.726
PRIMER_RIGHT_11_TM=60.742
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=681
PRIMER_PAIR_12_PENALTY=2.089266
PRIMER_LEFT_12_PENALTY=1.041614
PRIMER_RIGHT_12_PENALTY=1.047652
PRIMER_LEFT_12_SEQUENCE=GCCCACCAAACCAACTTACT
PRIMER_RIGHT_12_SEQUENCE=TTTTTCCGGACTTCGATACCT
PRIMER_LEFT_12=522,20
PRIMER_RIGHT_12=1197,21
PRIMER_LEFT_12_TM=58.958
PRIMER_RIGHT_12_TM=59.952
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=676
PRIMER_PAIR_13_PENALTY=2.097172
PRIMER_LEFT_13_PENALTY=1.819573
PRIMER_RIGHT_13_PENALTY=0.277599
PRIMER_LEFT_13_SEQUENCE=CCGGATTGGATCACCATTTT
PRIMER_RIGHT_13_SEQUENCE=GAAGATGGGGAATGGCATAA
PRIMER_LEFT_13=545,20
PRIMER_RIGHT_13=1167,20
PRIMER_LEFT_13_TM=61.820
PRIMER_RIGHT_13_TM=59.722
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.2000
PRIMER_RIGHT_13_END_STABILITY=6.2000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=623
PRIMER_PAIR_14_PENALTY=2.109974
PRIMER_LEFT_14_PENALTY=0.755541
PRIMER_RIGHT_14_PENALTY=1.354433
PRIMER_LEFT_14_SEQUENCE=CTAAACCGGATTGGATCACC
PRIMER_RIGHT_14_SEQUENCE=TTTTCCGGACTTCGATACCT
PRIMER_LEFT_14=540,20
PRIMER_RIGHT_14=1196,20
PRIMER_LEFT_14_TM=59.244
PRIMER_RIGHT_14_TM=58.646
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=657
PRIMER_PAIR_15_PENALTY=2.115005
PRIMER_LEFT_15_PENALTY=1.067353
PRIMER_RIGHT_15_PENALTY=1.047652
PRIMER_LEFT_15_SEQUENCE=ACTAAACCGGATTGGATCACC
PRIMER_RIGHT_15_SEQUENCE=TTTTTCCGGACTTCGATACCT
PRIMER_LEFT_15=539,21
PRIMER_RIGHT_15=1197,21
PRIMER_LEFT_15_TM=60.067
PRIMER_RIGHT_15_TM=59.952
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=659
PRIMER_PAIR_16_PENALTY=2.141350
PRIMER_LEFT_16_PENALTY=2.075027
PRIMER_RIGHT_16_PENALTY=0.066323
PRIMER_LEFT_16_SEQUENCE=TACTAAACCGGATTGGATCACC
PRIMER_RIGHT_16_SEQUENCE=TTTCCGGACTTCGATACCTG
PRIMER_LEFT_16=538,22
PRIMER_RIGHT_16=1195,20
PRIMER_LEFT_16_TM=60.075
PRIMER_RIGHT_16_TM=60.066
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=658
PRIMER_PAIR_17_PENALTY=2.200850
PRIMER_LEFT_17_PENALTY=0.755541
PRIMER_RIGHT_17_PENALTY=1.445309
PRIMER_LEFT_17_SEQUENCE=CTAAACCGGATTGGATCACC
PRIMER_RIGHT_17_SEQUENCE=GCATGATCTTTTTCCGGACTT
PRIMER_LEFT_17=540,20
PRIMER_RIGHT_17=1205,21
PRIMER_LEFT_17_TM=59.244
PRIMER_RIGHT_17_TM=60.445
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=666
PRIMER_PAIR_18_PENALTY=2.272001
PRIMER_LEFT_18_PENALTY=1.273836
PRIMER_RIGHT_18_PENALTY=0.998165
PRIMER_LEFT_18_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_18_SEQUENCE=CCATTGCTCGTAACCATGTC
PRIMER_LEFT_18=881,20
PRIMER_RIGHT_18=1740,20
PRIMER_LEFT_18_TM=58.726
PRIMER_RIGHT_18_TM=59.002
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=860
PRIMER_PAIR_19_PENALTY=2.320535
PRIMER_LEFT_19_PENALTY=1.273836
PRIMER_RIGHT_19_PENALTY=1.046699
PRIMER_LEFT_19_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_19_SEQUENCE=ACTGTTCCTTGCCTCGGTTT
PRIMER_LEFT_19=881,20
PRIMER_RIGHT_19=1818,20
PRIMER_LEFT_19_TM=58.726
PRIMER_RIGHT_19_TM=61.047
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=938
PRIMER_PAIR_20_PENALTY=2.352626
PRIMER_LEFT_20_PENALTY=2.075027
PRIMER_RIGHT_20_PENALTY=0.277599
PRIMER_LEFT_20_SEQUENCE=TACTAAACCGGATTGGATCACC
PRIMER_RIGHT_20_SEQUENCE=GAAGATGGGGAATGGCATAA
PRIMER_LEFT_20=538,22
PRIMER_RIGHT_20=1167,20
PRIMER_LEFT_20_TM=60.075
PRIMER_RIGHT_20_TM=59.722
PRIMER_LEFT_20_GC_PERCENT=45.455
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=630
PRIMER_PAIR_21_PENALTY=2.386205
PRIMER_LEFT_21_PENALTY=1.338553
PRIMER_RIGHT_21_PENALTY=1.047652
PRIMER_LEFT_21_SEQUENCE=ACCGGATTGGATCACCATTT
PRIMER_RIGHT_21_SEQUENCE=TTTTTCCGGACTTCGATACCT
PRIMER_LEFT_21=544,20
PRIMER_RIGHT_21=1197,21
PRIMER_LEFT_21_TM=61.339
PRIMER_RIGHT_21_TM=59.952
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.2000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=654
PRIMER_PAIR_22_PENALTY=2.396047
PRIMER_LEFT_22_PENALTY=1.041614
PRIMER_RIGHT_22_PENALTY=1.354433
PRIMER_LEFT_22_SEQUENCE=GCCCACCAAACCAACTTACT
PRIMER_RIGHT_22_SEQUENCE=TTTTCCGGACTTCGATACCT
PRIMER_LEFT_22=522,20
PRIMER_RIGHT_22=1196,20
PRIMER_LEFT_22_TM=58.958
PRIMER_RIGHT_22_TM=58.646
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.7000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=675
PRIMER_PAIR_23_PENALTY=2.421786
PRIMER_LEFT_23_PENALTY=1.067353
PRIMER_RIGHT_23_PENALTY=1.354433
PRIMER_LEFT_23_SEQUENCE=ACTAAACCGGATTGGATCACC
PRIMER_RIGHT_23_SEQUENCE=TTTTCCGGACTTCGATACCT
PRIMER_LEFT_23=539,21
PRIMER_RIGHT_23=1196,20
PRIMER_LEFT_23_TM=60.067
PRIMER_RIGHT_23_TM=58.646
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=658
PRIMER_PAIR_24_PENALTY=2.486924
PRIMER_LEFT_24_PENALTY=1.041614
PRIMER_RIGHT_24_PENALTY=1.445309
PRIMER_LEFT_24_SEQUENCE=GCCCACCAAACCAACTTACT
PRIMER_RIGHT_24_SEQUENCE=GCATGATCTTTTTCCGGACTT
PRIMER_LEFT_24=522,20
PRIMER_RIGHT_24=1205,21
PRIMER_LEFT_24_TM=58.958
PRIMER_RIGHT_24_TM=60.445
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=5.7000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=684
PRIMER_PAIR_25_PENALTY=2.512662
PRIMER_LEFT_25_PENALTY=1.067353
PRIMER_RIGHT_25_PENALTY=1.445309
PRIMER_LEFT_25_SEQUENCE=ACTAAACCGGATTGGATCACC
PRIMER_RIGHT_25_SEQUENCE=GCATGATCTTTTTCCGGACTT
PRIMER_LEFT_25=539,21
PRIMER_RIGHT_25=1205,21
PRIMER_LEFT_25_TM=60.067
PRIMER_RIGHT_25_TM=60.445
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=667
PRIMER_PAIR_26_PENALTY=2.549846
PRIMER_LEFT_26_PENALTY=1.273836
PRIMER_RIGHT_26_PENALTY=1.276010
PRIMER_LEFT_26_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_26_SEQUENCE=TGACTCCATTGCTCGTAACC
PRIMER_LEFT_26=881,20
PRIMER_RIGHT_26=1745,20
PRIMER_LEFT_26_TM=58.726
PRIMER_RIGHT_26_TM=58.724
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.9000
PRIMER_RIGHT_26_END_STABILITY=7.2000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=865
PRIMER_PAIR_27_PENALTY=2.661756
PRIMER_LEFT_27_PENALTY=1.273836
PRIMER_RIGHT_27_PENALTY=1.387920
PRIMER_LEFT_27_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_27_SEQUENCE=GTGACTCCATTGCTCGTAACC
PRIMER_LEFT_27=881,20
PRIMER_RIGHT_27=1746,21
PRIMER_LEFT_27_TM=58.726
PRIMER_RIGHT_27_TM=59.612
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.2000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=866
PRIMER_PAIR_28_PENALTY=2.692987
PRIMER_LEFT_28_PENALTY=1.338553
PRIMER_RIGHT_28_PENALTY=1.354433
PRIMER_LEFT_28_SEQUENCE=ACCGGATTGGATCACCATTT
PRIMER_RIGHT_28_SEQUENCE=TTTTCCGGACTTCGATACCT
PRIMER_LEFT_28=544,20
PRIMER_RIGHT_28=1196,20
PRIMER_LEFT_28_TM=61.339
PRIMER_RIGHT_28_TM=58.646
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=653
PRIMER_PAIR_29_PENALTY=2.783863
PRIMER_LEFT_29_PENALTY=1.338553
PRIMER_RIGHT_29_PENALTY=1.445309
PRIMER_LEFT_29_SEQUENCE=ACCGGATTGGATCACCATTT
PRIMER_RIGHT_29_SEQUENCE=GCATGATCTTTTTCCGGACTT
PRIMER_LEFT_29=544,20
PRIMER_RIGHT_29=1205,21
PRIMER_LEFT_29_TM=61.339
PRIMER_RIGHT_29_TM=60.445
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.2000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=662
PRIMER_PAIR_30_PENALTY=2.864111
PRIMER_LEFT_30_PENALTY=1.273836
PRIMER_RIGHT_30_PENALTY=1.590274
PRIMER_LEFT_30_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_30_SEQUENCE=GACTGTTCCTTGCCTCGGTT
PRIMER_LEFT_30=881,20
PRIMER_RIGHT_30=1819,20
PRIMER_LEFT_30_TM=58.726
PRIMER_RIGHT_30_TM=61.590
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=9.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=939
PRIMER_PAIR_31_PENALTY=2.867225
PRIMER_LEFT_31_PENALTY=1.819573
PRIMER_RIGHT_31_PENALTY=1.047652
PRIMER_LEFT_31_SEQUENCE=CCGGATTGGATCACCATTTT
PRIMER_RIGHT_31_SEQUENCE=TTTTTCCGGACTTCGATACCT
PRIMER_LEFT_31=545,20
PRIMER_RIGHT_31=1197,21
PRIMER_LEFT_31_TM=61.820
PRIMER_RIGHT_31_TM=59.952
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.2000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=653
PRIMER_PAIR_32_PENALTY=2.978197
PRIMER_LEFT_32_PENALTY=1.273836
PRIMER_RIGHT_32_PENALTY=1.704360
PRIMER_LEFT_32_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_32_SEQUENCE=TTGCTCGTAACCATGTCGAGT
PRIMER_LEFT_32=881,20
PRIMER_RIGHT_32=1737,21
PRIMER_LEFT_32_TM=58.726
PRIMER_RIGHT_32_TM=60.704
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=857
PRIMER_PAIR_33_PENALTY=3.086260
PRIMER_LEFT_33_PENALTY=0.755541
PRIMER_RIGHT_33_PENALTY=2.330719
PRIMER_LEFT_33_SEQUENCE=CTAAACCGGATTGGATCACC
PRIMER_RIGHT_33_SEQUENCE=TTTTCCGGACTTCGATACCTG
PRIMER_LEFT_33=540,20
PRIMER_RIGHT_33=1196,21
PRIMER_LEFT_33_TM=59.244
PRIMER_RIGHT_33_TM=61.331
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=657
PRIMER_PAIR_34_PENALTY=3.122679
PRIMER_LEFT_34_PENALTY=2.075027
PRIMER_RIGHT_34_PENALTY=1.047652
PRIMER_LEFT_34_SEQUENCE=TACTAAACCGGATTGGATCACC
PRIMER_RIGHT_34_SEQUENCE=TTTTTCCGGACTTCGATACCT
PRIMER_LEFT_34=538,22
PRIMER_RIGHT_34=1197,21
PRIMER_LEFT_34_TM=60.075
PRIMER_RIGHT_34_TM=59.952
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=660
PRIMER_PAIR_35_PENALTY=3.174006
PRIMER_LEFT_35_PENALTY=1.819573
PRIMER_RIGHT_35_PENALTY=1.354433
PRIMER_LEFT_35_SEQUENCE=CCGGATTGGATCACCATTTT
PRIMER_RIGHT_35_SEQUENCE=TTTTCCGGACTTCGATACCT
PRIMER_LEFT_35=545,20
PRIMER_RIGHT_35=1196,20
PRIMER_LEFT_35_TM=61.820
PRIMER_RIGHT_35_TM=58.646
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.2000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=652
PRIMER_PAIR_36_PENALTY=3.183281
PRIMER_LEFT_36_PENALTY=1.273836
PRIMER_RIGHT_36_PENALTY=1.909444
PRIMER_LEFT_36_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_36_SEQUENCE=TCCATTGCTCGTAACCATGTC
PRIMER_LEFT_36=881,20
PRIMER_RIGHT_36=1741,21
PRIMER_LEFT_36_TM=58.726
PRIMER_RIGHT_36_TM=60.909
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=6.3000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=861
PRIMER_PAIR_37_PENALTY=3.237156
PRIMER_LEFT_37_PENALTY=1.273836
PRIMER_RIGHT_37_PENALTY=1.963320
PRIMER_LEFT_37_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_37_SEQUENCE=ATAGATCGAAGCGTTGGCATT
PRIMER_LEFT_37=881,20
PRIMER_RIGHT_37=1562,21
PRIMER_LEFT_37_TM=58.726
PRIMER_RIGHT_37_TM=60.963
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=682
PRIMER_PAIR_38_PENALTY=3.240966
PRIMER_LEFT_38_PENALTY=1.273836
PRIMER_RIGHT_38_PENALTY=1.967130
PRIMER_LEFT_38_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_38_SEQUENCE=TGGAAATGGAAGCCTAATGAC
PRIMER_LEFT_38=881,20
PRIMER_RIGHT_38=1837,21
PRIMER_LEFT_38_TM=58.726
PRIMER_RIGHT_38_TM=59.033
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=957
PRIMER_PAIR_39_PENALTY=3.264882
PRIMER_LEFT_39_PENALTY=1.819573
PRIMER_RIGHT_39_PENALTY=1.445309
PRIMER_LEFT_39_SEQUENCE=CCGGATTGGATCACCATTTT
PRIMER_RIGHT_39_SEQUENCE=GCATGATCTTTTTCCGGACTT
PRIMER_LEFT_39=545,20
PRIMER_RIGHT_39=1205,21
PRIMER_LEFT_39_TM=61.820
PRIMER_RIGHT_39_TM=60.445
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=661
PRIMER_PAIR_40_PENALTY=3.275611
PRIMER_LEFT_40_PENALTY=1.273836
PRIMER_RIGHT_40_PENALTY=2.001775
PRIMER_LEFT_40_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_40_SEQUENCE=GCCTAATGACTGTTCCTTGCC
PRIMER_LEFT_40=881,20
PRIMER_RIGHT_40=1826,21
PRIMER_LEFT_40_TM=58.726
PRIMER_RIGHT_40_TM=61.002
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=10.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=946
PRIMER_PAIR_41_PENALTY=3.287237
PRIMER_LEFT_41_PENALTY=1.273836
PRIMER_RIGHT_41_PENALTY=2.013401
PRIMER_LEFT_41_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_41_SEQUENCE=TCTTGGGCTATAATCGGTGACT
PRIMER_LEFT_41=881,20
PRIMER_RIGHT_41=1762,22
PRIMER_LEFT_41_TM=58.726
PRIMER_RIGHT_41_TM=59.987
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=882
PRIMER_PAIR_42_PENALTY=3.307939
PRIMER_LEFT_42_PENALTY=1.273836
PRIMER_RIGHT_42_PENALTY=2.034102
PRIMER_LEFT_42_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_42_SEQUENCE=GGAAATGGAAGCCTAATGACTG
PRIMER_LEFT_42=881,20
PRIMER_RIGHT_42=1836,22
PRIMER_LEFT_42_TM=58.726
PRIMER_RIGHT_42_TM=59.966
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=956
PRIMER_PAIR_43_PENALTY=3.307939
PRIMER_LEFT_43_PENALTY=1.273836
PRIMER_RIGHT_43_PENALTY=2.034102
PRIMER_LEFT_43_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_43_SEQUENCE=TGGAAATGGAAGCCTAATGACT
PRIMER_LEFT_43=881,20
PRIMER_RIGHT_43=1837,22
PRIMER_LEFT_43_TM=58.726
PRIMER_RIGHT_43_TM=59.966
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=957
PRIMER_PAIR_44_PENALTY=3.309786
PRIMER_LEFT_44_PENALTY=2.628165
PRIMER_RIGHT_44_PENALTY=0.681621
PRIMER_LEFT_44_SEQUENCE=CAAGCACCAATGCAGACAATC
PRIMER_RIGHT_44_SEQUENCE=TGCTCGTAACCATGTCGAGT
PRIMER_LEFT_44=880,21
PRIMER_RIGHT_44=1736,20
PRIMER_LEFT_44_TM=61.628
PRIMER_RIGHT_44_TM=59.318
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=8.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=857
PRIMER_PAIR_45_PENALTY=3.369819
PRIMER_LEFT_45_PENALTY=2.628165
PRIMER_RIGHT_45_PENALTY=0.741655
PRIMER_LEFT_45_SEQUENCE=CAAGCACCAATGCAGACAATC
PRIMER_RIGHT_45_SEQUENCE=TAGATCGAAGCGTTGGCATT
PRIMER_LEFT_45=880,21
PRIMER_RIGHT_45=1561,20
PRIMER_LEFT_45_TM=61.628
PRIMER_RIGHT_45_TM=60.742
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=682
PRIMER_PAIR_46_PENALTY=3.372334
PRIMER_LEFT_46_PENALTY=1.041614
PRIMER_RIGHT_46_PENALTY=2.330719
PRIMER_LEFT_46_SEQUENCE=GCCCACCAAACCAACTTACT
PRIMER_RIGHT_46_SEQUENCE=TTTTCCGGACTTCGATACCTG
PRIMER_LEFT_46=522,20
PRIMER_RIGHT_46=1196,21
PRIMER_LEFT_46_TM=58.958
PRIMER_RIGHT_46_TM=61.331
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=5.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=675
PRIMER_PAIR_47_PENALTY=3.398072
PRIMER_LEFT_47_PENALTY=1.067353
PRIMER_RIGHT_47_PENALTY=2.330719
PRIMER_LEFT_47_SEQUENCE=ACTAAACCGGATTGGATCACC
PRIMER_RIGHT_47_SEQUENCE=TTTTCCGGACTTCGATACCTG
PRIMER_LEFT_47=539,21
PRIMER_RIGHT_47=1196,21
PRIMER_LEFT_47_TM=60.067
PRIMER_RIGHT_47_TM=61.331
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=658
PRIMER_PAIR_48_PENALTY=3.404194
PRIMER_LEFT_48_PENALTY=1.273836
PRIMER_RIGHT_48_PENALTY=2.130358
PRIMER_LEFT_48_SEQUENCE=AAGCACCAATGCAGACAATC
PRIMER_RIGHT_48_SEQUENCE=CCTAATGACTGTTCCTTGCCTC
PRIMER_LEFT_48=881,20
PRIMER_RIGHT_48=1825,22
PRIMER_LEFT_48_TM=58.726
PRIMER_RIGHT_48_TM=60.130
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=945
PRIMER_PAIR_49_PENALTY=3.428194
PRIMER_LEFT_49_PENALTY=3.150595
PRIMER_RIGHT_49_PENALTY=0.277599
PRIMER_LEFT_49_SEQUENCE=AAGGTGATCTATCCGGTTCATT
PRIMER_RIGHT_49_SEQUENCE=GAAGATGGGGAATGGCATAA
PRIMER_LEFT_49=668,22
PRIMER_RIGHT_49=1167,20
PRIMER_LEFT_49_TM=58.849
PRIMER_RIGHT_49_TM=59.722
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=6.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=500
=
SEQUENCE_ID=MAGIC.433_2_3921913_3922351_kn-0_sf-2_243495_46994_TRUE
SEQUENCE_TARGET=950,538
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_5495427_5495942_edi-0_rsch-4_122739_92618_TRUE
SEQUENCE_TARGET=950,615
SEQUENCE_TEMPLATE=TNAATTGGACCNNNNNNNNTTAAAGTATATAAAATNTATTGAATTGTTTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTGTCAAANAAATATGCGATTAACAATCTTAAGAGTCTCCACACATTCACAGAGAAACCGTAACCCGGCTAGAGNGTTACAGAACNGGACTTNTTCAGATNTCTCGTNTCCGGTTTTGTACAAGAACANCTTCTTNTTNGTACGCTCTTCAGGTTCCATCTCGAAATNATCGTNGTATCCANTGAATGTTACGANGAGATCGTTCTTTCTAGTAAACGACACGAATTCGANTTGATAATCGTAGATACGATCTTGTACAGAGATGAAANTCGACATTGAAAGGCTTATAATCTTGNTCCATGATCCGGTTTTCTCCATAACCGAAATCCGAATCNCCTTCTTGCACATTTCATACTCTGTAATACAGAGTTTTTNACTTGTAACGCCTAACCATGAAGNCGTCACGCCTTGGTTGTAAACCGGGAGAGGGANTTTTCGGTATTCATGNGTTGAGAGATTAAAGCANAGGATAAACCGGTCNNCANTAGACCGGTACGCAATCCAATAGAGGTATTGGTCCAAAAATGTACCTCTCCTGTCTCTGTAATGCCAATCAGGATAAGAAATACGTAGGCTCTCTCCCCATGAACCGGTTCTAAATTCGAAAACATGCGCCGTGGAAACATCAAGCCGGTCGATAAACGTTACGACTTTGTAATCGTCGTGAACCGGGTCATAACCGAAACCCAGAATGTTTGAATCTTGGTATATACCGGGATTTGGTACGATCTTGAATTGCTTCGAAAACGGATTCCAAACCATTAAAGTGTGATTCTTNAANGTAACACATAANGTCCCATCACAATGAAGCACACATGTAGATTCATCGATCATTGACTGATCCAANGGGAAATCGAGCTTGTTTGTAATAACCGGATGCTTGCCTTCAACGTCAAGNATGTAAAAGTTCTTGTCGTAAAACGACACGAACTTTGCCGGAGACATGTCTCGGTACGTTTCTGNGAATCCAGGGTCGTTGATAAGATCTCTCCATGTCTTGCANACGCATCTGAATCGAGCAAGATCCTTCATCGATAGCCTCACTANNATCTCAATGGCTAATTCTTCCGGGAGAAATTTCGACATTTGNTNTTTTTTGTGGTTGAAGACGTATCGGTATGGCTCAAAAAAGGGTNGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCATGATTTAGGTGCGATCCTTTCCGAAAATCAAAGTATTTNTCCTAATGCGATGAGAGCTTTGAATATCCAACCCATCTTCATAAGACACATCCTTGACTTATACAAAGAGTGAGAGTTGNNNNNNNNTTTCAGTTGAAGGAGATNCTTTAATTTGATTATATNNCACNTTTNATTTCTGCAACATACATATCCACAAGCTATGTNTATGCAATATTTTGAGANAAAAAAAAGTGCAAANA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_11956936_11956967_col-0_sf-2_78_738881_TRUE
SEQUENCE_TARGET=950,131
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNAACGTTCTATAATTTNTATNNCTTTNAATAATTTATTNAATTAATACTTAAATAATAAAGGGTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTNAANNANATAAATGGTATGTCTTTCACTTTGNACGGAGTATCCCNATGTGGGGTACATAAATATTAAGAGAAAAAAAGAGATCAAGCTTACTAGTTTAAGCGTTAATAATTATACTGACTGACCAATCTACTTGTTCATGTAGCTCTAATGTTATTAATTANTATATAGTCTTAGATTGATCTAATTAATGTCTGTGTATGATGTCACTTTGTGATGATGGAAGTCATGAGAGCTTCCATTACTTAACTCTCTCTCTCTCTNTCTATNTATNTTTACGGTCATGTATAGTGTAATCANNAATAACAAGAAAAAGCCAAGTTCAAAGCACNATACTATTAGTTAAAGACAAATGCGATAATGATACGATTCTGAGCATTACTTGAGTCNCAATATATAGTCACACCCTCATCAAATTCTTTNTATCTTACCAACTTCTNAAAAAAAANNAAAAANNNAAAAGACAAGTAAATACATANATAAATATACACNCACACACACACACATATATTGATATACTTATAATNATAAATCATGGTAAAGTTTGANACAAAACTATGGAAGGCTGTGCTTATGATGTCCATGATCAACATTGGTTTAAGTGTTGTAAACGTTATGTTCAAGAAGATGATTGATGAAGGACTTAACCGTATGGTCGCGACCACTTACCGACTTGCTGTAGGAACTCTGTTCTTNATACCATTCGCGATTTTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNTTNNNNNNNNTNTNNNNNGNTNNNTGTNTTNTTNNTTNTNNNNNTTNNANTTNTNNTTNAANTNTNNNNTTNAATNATAANNGGATGTCTTGTTTATACNANTNTCTAGTTGAATGTTGTTTTCAAACTCTTTTCTCCTGTGAATATGTNATTATATATTCGTATTCCATATGAAAGATTTTGNNNNNNNNNNNNNTTGTTCTTTTTTTNNNNNTCTCTTTTNGTAGACATAACAGGCCAAAACTCACGGGTAGGATCTTGTGTTCACTCTTCTTTAGCGCTCTTCTGGGGTATGTTCATTTTATTAGACTGTTCANTTATAATAAATGTTTACAAATAAGTTTTGAGTTGTTTNTTNTGATCTTGATCGTTNNTATATTTGCAGGACAAGTTTGGTGCAATACTTCTTTCTTATTGGACTAGAGTATACATCTTCAACNTTTTCCTTAGCNTTTAGCAACATGGTTCCTTCNGTTACNTTTGCNTTGGCTCTCGTCTTTAGGTANTNAAAAATCGATCAAACNCATTTGNATTCTTCATATATATNCTCTAGTATTATCNNAATNNTTNATAAACTANANGANTAGGCAAGAGACGCTGAACATCAAGAGCAACGTAGGAAGAGCCAAACTGCTAGGCACAATGATATGTATTTGTGGAGCTTTAGTGCTTACGCTTTATAAAGGAACCGCTTTAAGTCGAGAACANAGTACTCATATGNAAACACATACAAGAACTGATTCCACCGGTGCAATGACNCAAAAGTGGGCAATGGGTTCGATCATGCTGGTTATATCNATCATNATATGGTCATCTTGGTTCATTGTTCAAGCTAAAATAAGCNGANTTTATCCNTGCCAATACACGAGTACCACGATCCTCTCTTTCTTTGGCGTGATNCAATCTGCTTTGTTGAGTTTGATCTCGGAGAGGAGCACNTCTATGTGGGTCGTTAAAGACAAGTTCCAAGTTTTAGCTTTACTTTATTCGGTGAGTNATCCAATATACTATTTAATCAACCTACAAGATCTATATGTCAANATATATANAATTTTNTTTNAGNNNNNNNTTGTAAANAAAGNNAAAAAGAAANAAANAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.327749
PRIMER_LEFT_0_PENALTY=0.312496
PRIMER_RIGHT_0_PENALTY=0.015253
PRIMER_LEFT_0_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_0_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_0=814,20
PRIMER_RIGHT_0=1560,20
PRIMER_LEFT_0_TM=60.312
PRIMER_RIGHT_0_TM=60.015
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=747
PRIMER_PAIR_1_PENALTY=0.332263
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=0.019767
PRIMER_LEFT_1_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_1_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_1=814,20
PRIMER_RIGHT_1=1536,20
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=723
PRIMER_PAIR_2_PENALTY=0.337624
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_2_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_2=814,20
PRIMER_RIGHT_2=1224,20
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=411
PRIMER_PAIR_3_PENALTY=0.346176
PRIMER_LEFT_3_PENALTY=0.312496
PRIMER_RIGHT_3_PENALTY=0.033680
PRIMER_LEFT_3_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_3_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_3=814,20
PRIMER_RIGHT_3=1680,20
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=867
PRIMER_PAIR_4_PENALTY=0.346176
PRIMER_LEFT_4_PENALTY=0.312496
PRIMER_RIGHT_4_PENALTY=0.033680
PRIMER_LEFT_4_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_4_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_4=814,20
PRIMER_RIGHT_4=1681,20
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=868
PRIMER_PAIR_5_PENALTY=0.466519
PRIMER_LEFT_5_PENALTY=0.312496
PRIMER_RIGHT_5_PENALTY=0.154023
PRIMER_LEFT_5_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_5_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_5=814,20
PRIMER_RIGHT_5=1571,20
PRIMER_LEFT_5_TM=60.312
PRIMER_RIGHT_5_TM=60.154
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=758
PRIMER_PAIR_6_PENALTY=0.578933
PRIMER_LEFT_6_PENALTY=0.312496
PRIMER_RIGHT_6_PENALTY=0.266437
PRIMER_LEFT_6_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_6_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_6=814,20
PRIMER_RIGHT_6=1329,20
PRIMER_LEFT_6_TM=60.312
PRIMER_RIGHT_6_TM=59.734
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=516
PRIMER_PAIR_7_PENALTY=0.588245
PRIMER_LEFT_7_PENALTY=0.312496
PRIMER_RIGHT_7_PENALTY=0.275749
PRIMER_LEFT_7_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_7_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_7=814,20
PRIMER_RIGHT_7=1221,20
PRIMER_LEFT_7_TM=60.312
PRIMER_RIGHT_7_TM=59.724
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=408
PRIMER_PAIR_8_PENALTY=0.668069
PRIMER_LEFT_8_PENALTY=0.312496
PRIMER_RIGHT_8_PENALTY=0.355573
PRIMER_LEFT_8_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_8_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_8=814,20
PRIMER_RIGHT_8=1572,20
PRIMER_LEFT_8_TM=60.312
PRIMER_RIGHT_8_TM=59.644
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=759
PRIMER_PAIR_9_PENALTY=0.716170
PRIMER_LEFT_9_PENALTY=0.312496
PRIMER_RIGHT_9_PENALTY=0.403673
PRIMER_LEFT_9_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_9_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_9=814,20
PRIMER_RIGHT_9=1545,20
PRIMER_LEFT_9_TM=60.312
PRIMER_RIGHT_9_TM=59.596
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=732
PRIMER_PAIR_10_PENALTY=0.850580
PRIMER_LEFT_10_PENALTY=0.312496
PRIMER_RIGHT_10_PENALTY=0.538083
PRIMER_LEFT_10_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_10_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_10=814,20
PRIMER_RIGHT_10=1559,20
PRIMER_LEFT_10_TM=60.312
PRIMER_RIGHT_10_TM=60.538
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=746
PRIMER_PAIR_11_PENALTY=1.090076
PRIMER_LEFT_11_PENALTY=0.312496
PRIMER_RIGHT_11_PENALTY=0.777580
PRIMER_LEFT_11_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_11_SEQUENCE=GCCTAGCAGTTTGGCTCTTC
PRIMER_LEFT_11=814,20
PRIMER_RIGHT_11=1570,20
PRIMER_LEFT_11_TM=60.312
PRIMER_RIGHT_11_TM=59.222
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=757
PRIMER_PAIR_12_PENALTY=1.176120
PRIMER_LEFT_12_PENALTY=0.312496
PRIMER_RIGHT_12_PENALTY=0.863624
PRIMER_LEFT_12_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_12_SEQUENCE=TAACCAGCATGATCGAACCC
PRIMER_LEFT_12=814,20
PRIMER_RIGHT_12=1724,20
PRIMER_LEFT_12_TM=60.312
PRIMER_RIGHT_12_TM=60.864
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=911
PRIMER_PAIR_13_PENALTY=1.235854
PRIMER_LEFT_13_PENALTY=0.312496
PRIMER_RIGHT_13_PENALTY=0.923358
PRIMER_LEFT_13_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_13_SEQUENCE=CCTAGCAGTTTGGCTCTTCC
PRIMER_LEFT_13=814,20
PRIMER_RIGHT_13=1569,20
PRIMER_LEFT_13_TM=60.312
PRIMER_RIGHT_13_TM=59.077
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=756
PRIMER_PAIR_14_PENALTY=1.246241
PRIMER_LEFT_14_PENALTY=1.230989
PRIMER_RIGHT_14_PENALTY=0.015253
PRIMER_LEFT_14_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_14_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_14=853,20
PRIMER_RIGHT_14=1560,20
PRIMER_LEFT_14_TM=58.769
PRIMER_RIGHT_14_TM=60.015
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=708
PRIMER_PAIR_15_PENALTY=1.250755
PRIMER_LEFT_15_PENALTY=1.230989
PRIMER_RIGHT_15_PENALTY=0.019767
PRIMER_LEFT_15_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_15_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_15=853,20
PRIMER_RIGHT_15=1536,20
PRIMER_LEFT_15_TM=58.769
PRIMER_RIGHT_15_TM=60.020
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=684
PRIMER_PAIR_16_PENALTY=1.264669
PRIMER_LEFT_16_PENALTY=1.230989
PRIMER_RIGHT_16_PENALTY=0.033680
PRIMER_LEFT_16_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_16_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_16=853,20
PRIMER_RIGHT_16=1680,20
PRIMER_LEFT_16_TM=58.769
PRIMER_RIGHT_16_TM=59.966
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=828
PRIMER_PAIR_17_PENALTY=1.264669
PRIMER_LEFT_17_PENALTY=1.230989
PRIMER_RIGHT_17_PENALTY=0.033680
PRIMER_LEFT_17_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_17_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_17=853,20
PRIMER_RIGHT_17=1681,20
PRIMER_LEFT_17_TM=58.769
PRIMER_RIGHT_17_TM=59.966
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=829
PRIMER_PAIR_18_PENALTY=1.268471
PRIMER_LEFT_18_PENALTY=0.312496
PRIMER_RIGHT_18_PENALTY=0.955975
PRIMER_LEFT_18_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_18_SEQUENCE=GATGTTCAGCGTCTCTTGCC
PRIMER_LEFT_18=814,20
PRIMER_RIGHT_18=1537,20
PRIMER_LEFT_18_TM=60.312
PRIMER_RIGHT_18_TM=60.956
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=10.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=724
PRIMER_PAIR_19_PENALTY=1.338503
PRIMER_LEFT_19_PENALTY=0.312496
PRIMER_RIGHT_19_PENALTY=1.026007
PRIMER_LEFT_19_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_19_SEQUENCE=CCTAGCAGTTTGGCTCTTCCT
PRIMER_LEFT_19=814,20
PRIMER_RIGHT_19=1569,21
PRIMER_LEFT_19_TM=60.312
PRIMER_RIGHT_19_TM=60.026
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=756
PRIMER_PAIR_20_PENALTY=1.339574
PRIMER_LEFT_20_PENALTY=0.312496
PRIMER_RIGHT_20_PENALTY=1.027078
PRIMER_LEFT_20_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_20_SEQUENCE=TCGACTTAAAGCGGTTCCTT
PRIMER_LEFT_20=814,20
PRIMER_RIGHT_20=1633,20
PRIMER_LEFT_20_TM=60.312
PRIMER_RIGHT_20_TM=58.973
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=820
PRIMER_PAIR_21_PENALTY=1.345240
PRIMER_LEFT_21_PENALTY=0.312496
PRIMER_RIGHT_21_PENALTY=1.032744
PRIMER_LEFT_21_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_21_SEQUENCE=GGCTCTTCCTACGTTGCTCTT
PRIMER_LEFT_21=814,20
PRIMER_RIGHT_21=1558,21
PRIMER_LEFT_21_TM=60.312
PRIMER_RIGHT_21_TM=60.033
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=745
PRIMER_PAIR_22_PENALTY=1.385011
PRIMER_LEFT_22_PENALTY=1.230989
PRIMER_RIGHT_22_PENALTY=0.154023
PRIMER_LEFT_22_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_22_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_22=853,20
PRIMER_RIGHT_22=1571,20
PRIMER_LEFT_22_TM=58.769
PRIMER_RIGHT_22_TM=60.154
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=719
PRIMER_PAIR_23_PENALTY=1.412921
PRIMER_LEFT_23_PENALTY=0.312496
PRIMER_RIGHT_23_PENALTY=1.100425
PRIMER_LEFT_23_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_23_SEQUENCE=CTCGACTTAAAGCGGTTCCTT
PRIMER_LEFT_23=814,20
PRIMER_RIGHT_23=1634,21
PRIMER_LEFT_23_TM=60.312
PRIMER_RIGHT_23_TM=59.900
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=821
PRIMER_PAIR_24_PENALTY=1.469256
PRIMER_LEFT_24_PENALTY=0.312496
PRIMER_RIGHT_24_PENALTY=1.156760
PRIMER_LEFT_24_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_24_SEQUENCE=GCTCTTGATGTTCAGCGTCTC
PRIMER_LEFT_24=814,20
PRIMER_RIGHT_24=1543,21
PRIMER_LEFT_24_TM=60.312
PRIMER_RIGHT_24_TM=60.157
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=730
PRIMER_PAIR_25_PENALTY=1.497425
PRIMER_LEFT_25_PENALTY=1.230989
PRIMER_RIGHT_25_PENALTY=0.266437
PRIMER_LEFT_25_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_25_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_25=853,20
PRIMER_RIGHT_25=1329,20
PRIMER_LEFT_25_TM=58.769
PRIMER_RIGHT_25_TM=59.734
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=477
PRIMER_PAIR_26_PENALTY=1.542870
PRIMER_LEFT_26_PENALTY=0.312496
PRIMER_RIGHT_26_PENALTY=1.230374
PRIMER_LEFT_26_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_26_SEQUENCE=ATACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_26=814,20
PRIMER_RIGHT_26=1225,21
PRIMER_LEFT_26_TM=60.312
PRIMER_RIGHT_26_TM=60.230
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=6.3000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=412
PRIMER_PAIR_27_PENALTY=1.586561
PRIMER_LEFT_27_PENALTY=1.230989
PRIMER_RIGHT_27_PENALTY=0.355573
PRIMER_LEFT_27_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_27_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_27=853,20
PRIMER_RIGHT_27=1572,20
PRIMER_LEFT_27_TM=58.769
PRIMER_RIGHT_27_TM=59.644
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=720
PRIMER_PAIR_28_PENALTY=1.615228
PRIMER_LEFT_28_PENALTY=0.312496
PRIMER_RIGHT_28_PENALTY=1.302732
PRIMER_LEFT_28_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_28_SEQUENCE=GGCTCTTCCTACGTTGCTCT
PRIMER_LEFT_28=814,20
PRIMER_RIGHT_28=1558,20
PRIMER_LEFT_28_TM=60.312
PRIMER_RIGHT_28_TM=58.697
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=745
PRIMER_PAIR_29_PENALTY=1.634662
PRIMER_LEFT_29_PENALTY=1.230989
PRIMER_RIGHT_29_PENALTY=0.403673
PRIMER_LEFT_29_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_29_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_29=853,20
PRIMER_RIGHT_29=1545,20
PRIMER_LEFT_29_TM=58.769
PRIMER_RIGHT_29_TM=59.596
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=693
PRIMER_PAIR_30_PENALTY=1.644931
PRIMER_LEFT_30_PENALTY=1.629679
PRIMER_RIGHT_30_PENALTY=0.015253
PRIMER_LEFT_30_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_30_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_30=815,20
PRIMER_RIGHT_30=1560,20
PRIMER_LEFT_30_TM=58.370
PRIMER_RIGHT_30_TM=60.015
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=746
PRIMER_PAIR_31_PENALTY=1.649445
PRIMER_LEFT_31_PENALTY=1.629679
PRIMER_RIGHT_31_PENALTY=0.019767
PRIMER_LEFT_31_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_31_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_31=815,20
PRIMER_RIGHT_31=1536,20
PRIMER_LEFT_31_TM=58.370
PRIMER_RIGHT_31_TM=60.020
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=722
PRIMER_PAIR_32_PENALTY=1.663359
PRIMER_LEFT_32_PENALTY=1.629679
PRIMER_RIGHT_32_PENALTY=0.033680
PRIMER_LEFT_32_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_32_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_32=815,20
PRIMER_RIGHT_32=1680,20
PRIMER_LEFT_32_TM=58.370
PRIMER_RIGHT_32_TM=59.966
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=866
PRIMER_PAIR_33_PENALTY=1.663359
PRIMER_LEFT_33_PENALTY=1.629679
PRIMER_RIGHT_33_PENALTY=0.033680
PRIMER_LEFT_33_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_33_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_33=815,20
PRIMER_RIGHT_33=1681,20
PRIMER_LEFT_33_TM=58.370
PRIMER_RIGHT_33_TM=59.966
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=867
PRIMER_PAIR_34_PENALTY=1.689614
PRIMER_LEFT_34_PENALTY=0.312496
PRIMER_RIGHT_34_PENALTY=1.377118
PRIMER_LEFT_34_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_34_SEQUENCE=ATTGCACCAAACTTGTCCTG
PRIMER_LEFT_34=814,20
PRIMER_RIGHT_34=1333,20
PRIMER_LEFT_34_TM=60.312
PRIMER_RIGHT_34_TM=58.623
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=520
PRIMER_PAIR_35_PENALTY=1.689693
PRIMER_LEFT_35_PENALTY=1.674441
PRIMER_RIGHT_35_PENALTY=0.015253
PRIMER_LEFT_35_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_35_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_35=715,20
PRIMER_RIGHT_35=1560,20
PRIMER_LEFT_35_TM=58.326
PRIMER_RIGHT_35_TM=60.015
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=846
PRIMER_PAIR_36_PENALTY=1.691116
PRIMER_LEFT_36_PENALTY=0.312496
PRIMER_RIGHT_36_PENALTY=1.378620
PRIMER_LEFT_36_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_36_SEQUENCE=TCTCGACTTAAAGCGGTTCCT
PRIMER_LEFT_36=814,20
PRIMER_RIGHT_36=1635,21
PRIMER_LEFT_36_TM=60.312
PRIMER_RIGHT_36_TM=60.379
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=822
PRIMER_PAIR_37_PENALTY=1.694207
PRIMER_LEFT_37_PENALTY=1.674441
PRIMER_RIGHT_37_PENALTY=0.019767
PRIMER_LEFT_37_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_37_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_37=715,20
PRIMER_RIGHT_37=1536,20
PRIMER_LEFT_37_TM=58.326
PRIMER_RIGHT_37_TM=60.020
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=822
PRIMER_PAIR_38_PENALTY=1.699569
PRIMER_LEFT_38_PENALTY=1.674441
PRIMER_RIGHT_38_PENALTY=0.025128
PRIMER_LEFT_38_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_38_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_38=715,20
PRIMER_RIGHT_38=1224,20
PRIMER_LEFT_38_TM=58.326
PRIMER_RIGHT_38_TM=59.975
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=510
PRIMER_PAIR_39_PENALTY=1.708121
PRIMER_LEFT_39_PENALTY=1.674441
PRIMER_RIGHT_39_PENALTY=0.033680
PRIMER_LEFT_39_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_39_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_39=715,20
PRIMER_RIGHT_39=1680,20
PRIMER_LEFT_39_TM=58.326
PRIMER_RIGHT_39_TM=59.966
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=966
PRIMER_PAIR_40_PENALTY=1.708121
PRIMER_LEFT_40_PENALTY=1.674441
PRIMER_RIGHT_40_PENALTY=0.033680
PRIMER_LEFT_40_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_40_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_40=715,20
PRIMER_RIGHT_40=1681,20
PRIMER_LEFT_40_TM=58.326
PRIMER_RIGHT_40_TM=59.966
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=967
PRIMER_PAIR_41_PENALTY=1.710108
PRIMER_LEFT_41_PENALTY=0.312496
PRIMER_RIGHT_41_PENALTY=1.397612
PRIMER_LEFT_41_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_41_SEQUENCE=CTCGACTTAAAGCGGTTCCT
PRIMER_LEFT_41=814,20
PRIMER_RIGHT_41=1634,20
PRIMER_LEFT_41_TM=60.312
PRIMER_RIGHT_41_TM=58.602
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=821
PRIMER_PAIR_42_PENALTY=1.738510
PRIMER_LEFT_42_PENALTY=1.230989
PRIMER_RIGHT_42_PENALTY=0.507521
PRIMER_LEFT_42_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_42_SEQUENCE=CACGCCAAAGAAAGAGAGGA
PRIMER_LEFT_42=853,20
PRIMER_RIGHT_42=1828,20
PRIMER_LEFT_42_TM=58.769
PRIMER_RIGHT_42_TM=60.508
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=976
PRIMER_PAIR_43_PENALTY=1.738510
PRIMER_LEFT_43_PENALTY=1.230989
PRIMER_RIGHT_43_PENALTY=0.507521
PRIMER_LEFT_43_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_43_SEQUENCE=TCACGCCAAAGAAAGAGAGG
PRIMER_LEFT_43=853,20
PRIMER_RIGHT_43=1829,20
PRIMER_LEFT_43_TM=58.769
PRIMER_RIGHT_43_TM=60.508
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=977
PRIMER_PAIR_44_PENALTY=1.744180
PRIMER_LEFT_44_PENALTY=1.728928
PRIMER_RIGHT_44_PENALTY=0.015253
PRIMER_LEFT_44_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_44_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_44=815,21
PRIMER_RIGHT_44=1560,20
PRIMER_LEFT_44_TM=59.271
PRIMER_RIGHT_44_TM=60.015
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=746
PRIMER_PAIR_45_PENALTY=1.748694
PRIMER_LEFT_45_PENALTY=1.728928
PRIMER_RIGHT_45_PENALTY=0.019767
PRIMER_LEFT_45_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_45_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_45=815,21
PRIMER_RIGHT_45=1536,20
PRIMER_LEFT_45_TM=59.271
PRIMER_RIGHT_45_TM=60.020
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=722
PRIMER_PAIR_46_PENALTY=1.754056
PRIMER_LEFT_46_PENALTY=1.728928
PRIMER_RIGHT_46_PENALTY=0.025128
PRIMER_LEFT_46_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_46_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_46=815,21
PRIMER_RIGHT_46=1224,20
PRIMER_LEFT_46_TM=59.271
PRIMER_RIGHT_46_TM=59.975
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.3000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=410
PRIMER_PAIR_47_PENALTY=1.762608
PRIMER_LEFT_47_PENALTY=1.728928
PRIMER_RIGHT_47_PENALTY=0.033680
PRIMER_LEFT_47_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_47_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_47=815,21
PRIMER_RIGHT_47=1680,20
PRIMER_LEFT_47_TM=59.271
PRIMER_RIGHT_47_TM=59.966
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=866
PRIMER_PAIR_48_PENALTY=1.762608
PRIMER_LEFT_48_PENALTY=1.728928
PRIMER_RIGHT_48_PENALTY=0.033680
PRIMER_LEFT_48_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_48_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_48=815,21
PRIMER_RIGHT_48=1681,20
PRIMER_LEFT_48_TM=59.271
PRIMER_RIGHT_48_TM=59.966
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=867
PRIMER_PAIR_49_PENALTY=1.768043
PRIMER_LEFT_49_PENALTY=0.312496
PRIMER_RIGHT_49_PENALTY=1.455547
PRIMER_LEFT_49_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_49_SEQUENCE=GTTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_49=814,20
PRIMER_RIGHT_49=1546,21
PRIMER_LEFT_49_TM=60.312
PRIMER_RIGHT_49_TM=60.456
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=733
=
SEQUENCE_ID=MAGIC.426_3_9607006_9607068_sf-2_wil-2_8015_574477_TRUE
SEQUENCE_TARGET=950,162
SEQUENCE_TEMPLATE=CTTNTCCCAGTTNAANGGNAGACAGTCCCANTTATTNNNTCTCCAGNANACTNCTCGTGCTNCAGTNNNNNNNNNNNNNNNNNNTNGATNGTCCNNCTTTTGATTCCTGNTCCATAAGCNTTNTTCAATCACATGAAATTTTCCCTNNTTCANNATGACTTGTACTTGAGGGCAAAGGGAAANAANANTCTTNNACNTTGAATTCATAGNCANGACGNGGGAGAATATACGATGGATGAGTGATATGAGCTTNGGCTCNGNNTCNAGACTCAATNTTTCCGNAANCANNANAANAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNNNNNNNNNNNNNNNNNNNNNNNNNGGCTGAGAATCAANATCCATAGAGTTGAAGACAGAGANAGATTGATTNNTGAGTGANATGAGCTCCGACTNNGGCTTCGGCTGCAAATTCCANTCNACTGAGNTGNCCNGAGAGATTATATATCTACTGAGTGACAAGANNNNNNNNNNNNNNNNNNNNNNCNGTCTNTNAGACANNTTATCGTCCANAAAGATTAGTTGANTNATGANTGATTTGAGNTTCAGCTCCGGAGANTTGTCGACGAGGGATATTATTTTNTNAATGAGTGATATGAGCTCCGGCTGCGGCTTNATNCTCCATGTNTCCNTGATTAGTGNCATGAGNNTANANTNCGGNNNNNGCTNNTCCCAANNTGNAGAGTTGACNAANNGCGTTCNAGAAANGAGTGATATGAGCNTCCTNAGCGNNTTNGGCTGGGACTCCAAATCCATNTCCNTGAAANAANCGATTGTTTCAGAGATGAGTGACATCAGCTCCNTCTCCGTCTNNNNNNNCNTTGACTTAACCAGAGNGATTNTTTGANTAATCANTNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNAANNCATTGANNNNNANGATNAGTAGTATGANCTTCNCCTCCGGATCCATCGNGANTTNCCGAGANNAAATTGTGAGANTANGATGAGAGAATTTTCANTATGAATGNAANTTANTGNTTGCTCTCTGTGTTTGTGAATGCTAATTGTGTAATATTTTGCAAATATTNNTAAANAGACAATCTCAANCTTNTCCGAGACGAGCCGACCACGTCGTNTTNNTNAACAGAGTAAGTAAATTCATTCANGTACTNNAGTNCCGGNTNAAAGAAAAACAAANATTAGATCANNTTNAAAAATNTGNAAATTTANAATTTAAATATTANNAAAATACATTTNTNTTCTGCANGTCTATTTAGACANGCTATTATATGANTAAAGNGTAATTTTCTTATNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTTCTTAAGCTTGTGTGAATACAGCGAAAGAATATATTATTACTCTTACAGTTCTCTAGATTCACGNNNNNNNNNNNNNNNNNNNNNNNTCGATTGTCCCGGCCATGTTAGTTTGGTTACTNCGACTTCNNTATTTTNTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAGNCAGCTAAAAANTATNAAGCGCTTAATTTNTGTTTATNNNCACTTGTTTNTATCATAANAGTANTAATAATGATTACTGTATTTANGNGATANNACAAAAACAAATAAANTNNAATNNNTAAATGACAATAAAATTGTTTGTACATGTNAAAAAAAAGNACATCACNAATTGTTACGATCANAAAATTGATGGTTCTAACCAAAATAATGTTAGGTTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=7.502630
PRIMER_LEFT_0_PENALTY=4.477178
PRIMER_RIGHT_0_PENALTY=3.025451
PRIMER_LEFT_0_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_0_SEQUENCE=CATTCACAAACACAGAGAGCAA
PRIMER_LEFT_0=218,22
PRIMER_RIGHT_0=1187,22
PRIMER_LEFT_0_TM=57.523
PRIMER_RIGHT_0_TM=58.975
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=970
PRIMER_PAIR_1_PENALTY=7.578711
PRIMER_LEFT_1_PENALTY=4.553260
PRIMER_RIGHT_1_PENALTY=3.025451
PRIMER_LEFT_1_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_1_SEQUENCE=CATTCACAAACACAGAGAGCAA
PRIMER_LEFT_1=218,21
PRIMER_RIGHT_1=1187,22
PRIMER_LEFT_1_TM=56.447
PRIMER_RIGHT_1_TM=58.975
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=970
PRIMER_PAIR_2_PENALTY=7.652859
PRIMER_LEFT_2_PENALTY=4.627407
PRIMER_RIGHT_2_PENALTY=3.025451
PRIMER_LEFT_2_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_2_SEQUENCE=CATTCACAAACACAGAGAGCAA
PRIMER_LEFT_2=218,23
PRIMER_RIGHT_2=1187,22
PRIMER_LEFT_2_TM=58.373
PRIMER_RIGHT_2_TM=58.975
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=970
PRIMER_PAIR_3_PENALTY=7.666760
PRIMER_LEFT_3_PENALTY=4.641309
PRIMER_RIGHT_3_PENALTY=3.025451
PRIMER_LEFT_3_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_3_SEQUENCE=CATTCACAAACACAGAGAGCAA
PRIMER_LEFT_3=219,24
PRIMER_RIGHT_3=1187,22
PRIMER_LEFT_3_TM=59.359
PRIMER_RIGHT_3_TM=58.975
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=969
PRIMER_PAIR_4_PENALTY=7.890590
PRIMER_LEFT_4_PENALTY=4.477178
PRIMER_RIGHT_4_PENALTY=3.413412
PRIMER_LEFT_4_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_4_SEQUENCE=GCATTCACAAACACAGAGAGCA
PRIMER_LEFT_4=218,22
PRIMER_RIGHT_4=1188,22
PRIMER_LEFT_4_TM=57.523
PRIMER_RIGHT_4_TM=61.413
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=971
PRIMER_PAIR_5_PENALTY=7.936505
PRIMER_LEFT_5_PENALTY=4.911054
PRIMER_RIGHT_5_PENALTY=3.025451
PRIMER_LEFT_5_SEQUENCE=GGGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_5_SEQUENCE=CATTCACAAACACAGAGAGCAA
PRIMER_LEFT_5=218,24
PRIMER_RIGHT_5=1187,22
PRIMER_LEFT_5_TM=60.911
PRIMER_RIGHT_5_TM=58.975
PRIMER_LEFT_5_GC_PERCENT=45.833
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=970
PRIMER_PAIR_6_PENALTY=7.966672
PRIMER_LEFT_6_PENALTY=4.553260
PRIMER_RIGHT_6_PENALTY=3.413412
PRIMER_LEFT_6_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_6_SEQUENCE=GCATTCACAAACACAGAGAGCA
PRIMER_LEFT_6=218,21
PRIMER_RIGHT_6=1188,22
PRIMER_LEFT_6_TM=56.447
PRIMER_RIGHT_6_TM=61.413
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=971
PRIMER_PAIR_7_PENALTY=8.022648
PRIMER_LEFT_7_PENALTY=4.477178
PRIMER_RIGHT_7_PENALTY=3.545470
PRIMER_LEFT_7_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_7_SEQUENCE=CATTCACAAACACAGAGAGCA
PRIMER_LEFT_7=218,22
PRIMER_RIGHT_7=1187,21
PRIMER_LEFT_7_TM=57.523
PRIMER_RIGHT_7_TM=57.455
PRIMER_LEFT_7_GC_PERCENT=45.455
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=970
PRIMER_PAIR_8_PENALTY=8.040819
PRIMER_LEFT_8_PENALTY=4.627407
PRIMER_RIGHT_8_PENALTY=3.413412
PRIMER_LEFT_8_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_8_SEQUENCE=GCATTCACAAACACAGAGAGCA
PRIMER_LEFT_8=218,23
PRIMER_RIGHT_8=1188,22
PRIMER_LEFT_8_TM=58.373
PRIMER_RIGHT_8_TM=61.413
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=8.054721
PRIMER_LEFT_9_PENALTY=4.641309
PRIMER_RIGHT_9_PENALTY=3.413412
PRIMER_LEFT_9_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_9_SEQUENCE=GCATTCACAAACACAGAGAGCA
PRIMER_LEFT_9=219,24
PRIMER_RIGHT_9=1188,22
PRIMER_LEFT_9_TM=59.359
PRIMER_RIGHT_9_TM=61.413
PRIMER_LEFT_9_GC_PERCENT=41.667
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=970
PRIMER_PAIR_10_PENALTY=8.098730
PRIMER_LEFT_10_PENALTY=4.553260
PRIMER_RIGHT_10_PENALTY=3.545470
PRIMER_LEFT_10_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_10_SEQUENCE=CATTCACAAACACAGAGAGCA
PRIMER_LEFT_10=218,21
PRIMER_RIGHT_10=1187,21
PRIMER_LEFT_10_TM=56.447
PRIMER_RIGHT_10_TM=57.455
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=970
PRIMER_PAIR_11_PENALTY=8.172877
PRIMER_LEFT_11_PENALTY=4.627407
PRIMER_RIGHT_11_PENALTY=3.545470
PRIMER_LEFT_11_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_11_SEQUENCE=CATTCACAAACACAGAGAGCA
PRIMER_LEFT_11=218,23
PRIMER_RIGHT_11=1187,21
PRIMER_LEFT_11_TM=58.373
PRIMER_RIGHT_11_TM=57.455
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=970
PRIMER_PAIR_12_PENALTY=8.186779
PRIMER_LEFT_12_PENALTY=4.641309
PRIMER_RIGHT_12_PENALTY=3.545470
PRIMER_LEFT_12_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_12_SEQUENCE=CATTCACAAACACAGAGAGCA
PRIMER_LEFT_12=219,24
PRIMER_RIGHT_12=1187,21
PRIMER_LEFT_12_TM=59.359
PRIMER_RIGHT_12_TM=57.455
PRIMER_LEFT_12_GC_PERCENT=41.667
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=969
PRIMER_PAIR_13_PENALTY=8.324465
PRIMER_LEFT_13_PENALTY=4.911054
PRIMER_RIGHT_13_PENALTY=3.413412
PRIMER_LEFT_13_SEQUENCE=GGGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_13_SEQUENCE=GCATTCACAAACACAGAGAGCA
PRIMER_LEFT_13=218,24
PRIMER_RIGHT_13=1188,22
PRIMER_LEFT_13_TM=60.911
PRIMER_RIGHT_13_TM=61.413
PRIMER_LEFT_13_GC_PERCENT=45.833
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=971
PRIMER_PAIR_14_PENALTY=8.433998
PRIMER_LEFT_14_PENALTY=5.408547
PRIMER_RIGHT_14_PENALTY=3.025451
PRIMER_LEFT_14_SEQUENCE=GGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_14_SEQUENCE=CATTCACAAACACAGAGAGCAA
PRIMER_LEFT_14=219,23
PRIMER_RIGHT_14=1187,22
PRIMER_LEFT_14_TM=57.591
PRIMER_RIGHT_14_TM=58.975
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=969
PRIMER_PAIR_15_PENALTY=8.456524
PRIMER_LEFT_15_PENALTY=4.911054
PRIMER_RIGHT_15_PENALTY=3.545470
PRIMER_LEFT_15_SEQUENCE=GGGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_15_SEQUENCE=CATTCACAAACACAGAGAGCA
PRIMER_LEFT_15=218,24
PRIMER_RIGHT_15=1187,21
PRIMER_LEFT_15_TM=60.911
PRIMER_RIGHT_15_TM=57.455
PRIMER_LEFT_15_GC_PERCENT=45.833
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=970
PRIMER_PAIR_16_PENALTY=8.821959
PRIMER_LEFT_16_PENALTY=5.408547
PRIMER_RIGHT_16_PENALTY=3.413412
PRIMER_LEFT_16_SEQUENCE=GGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_16_SEQUENCE=GCATTCACAAACACAGAGAGCA
PRIMER_LEFT_16=219,23
PRIMER_RIGHT_16=1188,22
PRIMER_LEFT_16_TM=57.591
PRIMER_RIGHT_16_TM=61.413
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=970
PRIMER_PAIR_17_PENALTY=8.954017
PRIMER_LEFT_17_PENALTY=5.408547
PRIMER_RIGHT_17_PENALTY=3.545470
PRIMER_LEFT_17_SEQUENCE=GGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_17_SEQUENCE=CATTCACAAACACAGAGAGCA
PRIMER_LEFT_17=219,23
PRIMER_RIGHT_17=1187,21
PRIMER_LEFT_17_TM=57.591
PRIMER_RIGHT_17_TM=57.455
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=969
PRIMER_PAIR_18_PENALTY=9.058390
PRIMER_LEFT_18_PENALTY=4.477178
PRIMER_RIGHT_18_PENALTY=4.581212
PRIMER_LEFT_18_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_18_SEQUENCE=TTCACAAACACAGAGAGCAA
PRIMER_LEFT_18=218,22
PRIMER_RIGHT_18=1185,20
PRIMER_LEFT_18_TM=57.523
PRIMER_RIGHT_18_TM=55.419
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=968
PRIMER_PAIR_19_PENALTY=9.134472
PRIMER_LEFT_19_PENALTY=4.553260
PRIMER_RIGHT_19_PENALTY=4.581212
PRIMER_LEFT_19_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_19_SEQUENCE=TTCACAAACACAGAGAGCAA
PRIMER_LEFT_19=218,21
PRIMER_RIGHT_19=1185,20
PRIMER_LEFT_19_TM=56.447
PRIMER_RIGHT_19_TM=55.419
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=968
PRIMER_PAIR_20_PENALTY=9.208619
PRIMER_LEFT_20_PENALTY=4.627407
PRIMER_RIGHT_20_PENALTY=4.581212
PRIMER_LEFT_20_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_20_SEQUENCE=TTCACAAACACAGAGAGCAA
PRIMER_LEFT_20=218,23
PRIMER_RIGHT_20=1185,20
PRIMER_LEFT_20_TM=58.373
PRIMER_RIGHT_20_TM=55.419
PRIMER_LEFT_20_GC_PERCENT=43.478
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=968
PRIMER_PAIR_21_PENALTY=9.222521
PRIMER_LEFT_21_PENALTY=4.641309
PRIMER_RIGHT_21_PENALTY=4.581212
PRIMER_LEFT_21_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_21_SEQUENCE=TTCACAAACACAGAGAGCAA
PRIMER_LEFT_21=219,24
PRIMER_RIGHT_21=1185,20
PRIMER_LEFT_21_TM=59.359
PRIMER_RIGHT_21_TM=55.419
PRIMER_LEFT_21_GC_PERCENT=41.667
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=967
PRIMER_PAIR_22_PENALTY=9.492266
PRIMER_LEFT_22_PENALTY=4.911054
PRIMER_RIGHT_22_PENALTY=4.581212
PRIMER_LEFT_22_SEQUENCE=GGGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_22_SEQUENCE=TTCACAAACACAGAGAGCAA
PRIMER_LEFT_22=218,24
PRIMER_RIGHT_22=1185,20
PRIMER_LEFT_22_TM=60.911
PRIMER_RIGHT_22_TM=55.419
PRIMER_LEFT_22_GC_PERCENT=45.833
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=968
PRIMER_PAIR_23_PENALTY=9.762831
PRIMER_LEFT_23_PENALTY=4.477178
PRIMER_RIGHT_23_PENALTY=5.285652
PRIMER_LEFT_23_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_23_SEQUENCE=AGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_23=218,22
PRIMER_RIGHT_23=1189,23
PRIMER_LEFT_23_TM=57.523
PRIMER_RIGHT_23_TM=62.286
PRIMER_LEFT_23_GC_PERCENT=45.455
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=972
PRIMER_PAIR_24_PENALTY=9.838912
PRIMER_LEFT_24_PENALTY=4.553260
PRIMER_RIGHT_24_PENALTY=5.285652
PRIMER_LEFT_24_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_24_SEQUENCE=AGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_24=218,21
PRIMER_RIGHT_24=1189,23
PRIMER_LEFT_24_TM=56.447
PRIMER_RIGHT_24_TM=62.286
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=43.478
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=972
PRIMER_PAIR_25_PENALTY=9.913060
PRIMER_LEFT_25_PENALTY=4.627407
PRIMER_RIGHT_25_PENALTY=5.285652
PRIMER_LEFT_25_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_25_SEQUENCE=AGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_25=218,23
PRIMER_RIGHT_25=1189,23
PRIMER_LEFT_25_TM=58.373
PRIMER_RIGHT_25_TM=62.286
PRIMER_LEFT_25_GC_PERCENT=43.478
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=972
PRIMER_PAIR_26_PENALTY=9.926961
PRIMER_LEFT_26_PENALTY=4.641309
PRIMER_RIGHT_26_PENALTY=5.285652
PRIMER_LEFT_26_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_26_SEQUENCE=AGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_26=219,24
PRIMER_RIGHT_26=1189,23
PRIMER_LEFT_26_TM=59.359
PRIMER_RIGHT_26_TM=62.286
PRIMER_LEFT_26_GC_PERCENT=41.667
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=971
PRIMER_PAIR_27_PENALTY=9.932514
PRIMER_LEFT_27_PENALTY=4.477178
PRIMER_RIGHT_27_PENALTY=5.455336
PRIMER_LEFT_27_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_27_SEQUENCE=AGCATTCACAAACACAGAGAG
PRIMER_LEFT_27=218,22
PRIMER_RIGHT_27=1189,21
PRIMER_LEFT_27_TM=57.523
PRIMER_RIGHT_27_TM=55.545
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.6000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=972
PRIMER_PAIR_28_PENALTY=9.989759
PRIMER_LEFT_28_PENALTY=5.408547
PRIMER_RIGHT_28_PENALTY=4.581212
PRIMER_LEFT_28_SEQUENCE=GGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_28_SEQUENCE=TTCACAAACACAGAGAGCAA
PRIMER_LEFT_28=219,23
PRIMER_RIGHT_28=1185,20
PRIMER_LEFT_28_TM=57.591
PRIMER_RIGHT_28_TM=55.419
PRIMER_LEFT_28_GC_PERCENT=43.478
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=967
PRIMER_PAIR_29_PENALTY=10.008596
PRIMER_LEFT_29_PENALTY=4.553260
PRIMER_RIGHT_29_PENALTY=5.455336
PRIMER_LEFT_29_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_29_SEQUENCE=AGCATTCACAAACACAGAGAG
PRIMER_LEFT_29=218,21
PRIMER_RIGHT_29=1189,21
PRIMER_LEFT_29_TM=56.447
PRIMER_RIGHT_29_TM=55.545
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=972
PRIMER_PAIR_30_PENALTY=10.082743
PRIMER_LEFT_30_PENALTY=4.627407
PRIMER_RIGHT_30_PENALTY=5.455336
PRIMER_LEFT_30_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_30_SEQUENCE=AGCATTCACAAACACAGAGAG
PRIMER_LEFT_30=218,23
PRIMER_RIGHT_30=1189,21
PRIMER_LEFT_30_TM=58.373
PRIMER_RIGHT_30_TM=55.545
PRIMER_LEFT_30_GC_PERCENT=43.478
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=972
PRIMER_PAIR_31_PENALTY=10.096645
PRIMER_LEFT_31_PENALTY=4.641309
PRIMER_RIGHT_31_PENALTY=5.455336
PRIMER_LEFT_31_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_31_SEQUENCE=AGCATTCACAAACACAGAGAG
PRIMER_LEFT_31=219,24
PRIMER_RIGHT_31=1189,21
PRIMER_LEFT_31_TM=59.359
PRIMER_RIGHT_31_TM=55.545
PRIMER_LEFT_31_GC_PERCENT=41.667
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=971
PRIMER_PAIR_32_PENALTY=10.110918
PRIMER_LEFT_32_PENALTY=4.477178
PRIMER_RIGHT_32_PENALTY=5.633740
PRIMER_LEFT_32_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_32_SEQUENCE=GCATTCACAAACACAGAGAGCAA
PRIMER_LEFT_32=218,22
PRIMER_RIGHT_32=1188,23
PRIMER_LEFT_32_TM=57.523
PRIMER_RIGHT_32_TM=62.634
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=43.478
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=971
PRIMER_PAIR_33_PENALTY=10.187000
PRIMER_LEFT_33_PENALTY=4.553260
PRIMER_RIGHT_33_PENALTY=5.633740
PRIMER_LEFT_33_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_33_SEQUENCE=GCATTCACAAACACAGAGAGCAA
PRIMER_LEFT_33=218,21
PRIMER_RIGHT_33=1188,23
PRIMER_LEFT_33_TM=56.447
PRIMER_RIGHT_33_TM=62.634
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=43.478
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.6000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=971
PRIMER_PAIR_34_PENALTY=10.196706
PRIMER_LEFT_34_PENALTY=4.911054
PRIMER_RIGHT_34_PENALTY=5.285652
PRIMER_LEFT_34_SEQUENCE=GGGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_34_SEQUENCE=AGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_34=218,24
PRIMER_RIGHT_34=1189,23
PRIMER_LEFT_34_TM=60.911
PRIMER_RIGHT_34_TM=62.286
PRIMER_LEFT_34_GC_PERCENT=45.833
PRIMER_RIGHT_34_GC_PERCENT=43.478
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=972
PRIMER_PAIR_35_PENALTY=10.261147
PRIMER_LEFT_35_PENALTY=4.627407
PRIMER_RIGHT_35_PENALTY=5.633740
PRIMER_LEFT_35_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_35_SEQUENCE=GCATTCACAAACACAGAGAGCAA
PRIMER_LEFT_35=218,23
PRIMER_RIGHT_35=1188,23
PRIMER_LEFT_35_TM=58.373
PRIMER_RIGHT_35_TM=62.634
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=43.478
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=971
PRIMER_PAIR_36_PENALTY=10.275049
PRIMER_LEFT_36_PENALTY=4.641309
PRIMER_RIGHT_36_PENALTY=5.633740
PRIMER_LEFT_36_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_36_SEQUENCE=GCATTCACAAACACAGAGAGCAA
PRIMER_LEFT_36=219,24
PRIMER_RIGHT_36=1188,23
PRIMER_LEFT_36_TM=59.359
PRIMER_RIGHT_36_TM=62.634
PRIMER_LEFT_36_GC_PERCENT=41.667
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=970
PRIMER_PAIR_37_PENALTY=10.366390
PRIMER_LEFT_37_PENALTY=4.911054
PRIMER_RIGHT_37_PENALTY=5.455336
PRIMER_LEFT_37_SEQUENCE=GGGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_37_SEQUENCE=AGCATTCACAAACACAGAGAG
PRIMER_LEFT_37=218,24
PRIMER_RIGHT_37=1189,21
PRIMER_LEFT_37_TM=60.911
PRIMER_RIGHT_37_TM=55.545
PRIMER_LEFT_37_GC_PERCENT=45.833
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=972
PRIMER_PAIR_38_PENALTY=10.521503
PRIMER_LEFT_38_PENALTY=7.496052
PRIMER_RIGHT_38_PENALTY=3.025451
PRIMER_LEFT_38_SEQUENCE=GGGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_38_SEQUENCE=CATTCACAAACACAGAGAGCAA
PRIMER_LEFT_38=218,25
PRIMER_RIGHT_38=1187,22
PRIMER_LEFT_38_TM=62.496
PRIMER_RIGHT_38_TM=58.975
PRIMER_LEFT_38_GC_PERCENT=44.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=970
PRIMER_PAIR_39_PENALTY=10.544793
PRIMER_LEFT_39_PENALTY=4.911054
PRIMER_RIGHT_39_PENALTY=5.633740
PRIMER_LEFT_39_SEQUENCE=GGGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_39_SEQUENCE=GCATTCACAAACACAGAGAGCAA
PRIMER_LEFT_39=218,24
PRIMER_RIGHT_39=1188,23
PRIMER_LEFT_39_TM=60.911
PRIMER_RIGHT_39_TM=62.634
PRIMER_LEFT_39_GC_PERCENT=45.833
PRIMER_RIGHT_39_GC_PERCENT=43.478
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=971
PRIMER_PAIR_40_PENALTY=10.690208
PRIMER_LEFT_40_PENALTY=4.477178
PRIMER_RIGHT_40_PENALTY=6.213030
PRIMER_LEFT_40_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_40_SEQUENCE=TAGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_40=218,22
PRIMER_RIGHT_40=1190,24
PRIMER_LEFT_40_TM=57.523
PRIMER_RIGHT_40_TM=62.213
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=41.667
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.6000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=973
PRIMER_PAIR_41_PENALTY=10.694199
PRIMER_LEFT_41_PENALTY=5.408547
PRIMER_RIGHT_41_PENALTY=5.285652
PRIMER_LEFT_41_SEQUENCE=GGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_41_SEQUENCE=AGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_41=219,23
PRIMER_RIGHT_41=1189,23
PRIMER_LEFT_41_TM=57.591
PRIMER_RIGHT_41_TM=62.286
PRIMER_LEFT_41_GC_PERCENT=43.478
PRIMER_RIGHT_41_GC_PERCENT=43.478
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=971
PRIMER_PAIR_42_PENALTY=10.744805
PRIMER_LEFT_42_PENALTY=4.477178
PRIMER_RIGHT_42_PENALTY=6.267626
PRIMER_LEFT_42_SEQUENCE=GGGAGAATATACGATGGATGAG
PRIMER_RIGHT_42_SEQUENCE=TAGCATTCACAAACACAGAGAG
PRIMER_LEFT_42=218,22
PRIMER_RIGHT_42=1190,22
PRIMER_LEFT_42_TM=57.523
PRIMER_RIGHT_42_TM=55.732
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=973
PRIMER_PAIR_43_PENALTY=10.766290
PRIMER_LEFT_43_PENALTY=4.553260
PRIMER_RIGHT_43_PENALTY=6.213030
PRIMER_LEFT_43_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_43_SEQUENCE=TAGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_43=218,21
PRIMER_RIGHT_43=1190,24
PRIMER_LEFT_43_TM=56.447
PRIMER_RIGHT_43_TM=62.213
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=41.667
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=973
PRIMER_PAIR_44_PENALTY=10.820886
PRIMER_LEFT_44_PENALTY=4.553260
PRIMER_RIGHT_44_PENALTY=6.267626
PRIMER_LEFT_44_SEQUENCE=GGGAGAATATACGATGGATGA
PRIMER_RIGHT_44_SEQUENCE=TAGCATTCACAAACACAGAGAG
PRIMER_LEFT_44=218,21
PRIMER_RIGHT_44=1190,22
PRIMER_LEFT_44_TM=56.447
PRIMER_RIGHT_44_TM=55.732
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.6000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=973
PRIMER_PAIR_45_PENALTY=10.840437
PRIMER_LEFT_45_PENALTY=4.627407
PRIMER_RIGHT_45_PENALTY=6.213030
PRIMER_LEFT_45_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_45_SEQUENCE=TAGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_45=218,23
PRIMER_RIGHT_45=1190,24
PRIMER_LEFT_45_TM=58.373
PRIMER_RIGHT_45_TM=62.213
PRIMER_LEFT_45_GC_PERCENT=43.478
PRIMER_RIGHT_45_GC_PERCENT=41.667
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=973
PRIMER_PAIR_46_PENALTY=10.854339
PRIMER_LEFT_46_PENALTY=4.641309
PRIMER_RIGHT_46_PENALTY=6.213030
PRIMER_LEFT_46_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_46_SEQUENCE=TAGCATTCACAAACACAGAGAGCA
PRIMER_LEFT_46=219,24
PRIMER_RIGHT_46=1190,24
PRIMER_LEFT_46_TM=59.359
PRIMER_RIGHT_46_TM=62.213
PRIMER_LEFT_46_GC_PERCENT=41.667
PRIMER_RIGHT_46_GC_PERCENT=41.667
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=972
PRIMER_PAIR_47_PENALTY=10.863883
PRIMER_LEFT_47_PENALTY=5.408547
PRIMER_RIGHT_47_PENALTY=5.455336
PRIMER_LEFT_47_SEQUENCE=GGAGAATATACGATGGATGAGTG
PRIMER_RIGHT_47_SEQUENCE=AGCATTCACAAACACAGAGAG
PRIMER_LEFT_47=219,23
PRIMER_RIGHT_47=1189,21
PRIMER_LEFT_47_TM=57.591
PRIMER_RIGHT_47_TM=55.545
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=971
PRIMER_PAIR_48_PENALTY=10.895034
PRIMER_LEFT_48_PENALTY=4.627407
PRIMER_RIGHT_48_PENALTY=6.267626
PRIMER_LEFT_48_SEQUENCE=GGGAGAATATACGATGGATGAGT
PRIMER_RIGHT_48_SEQUENCE=TAGCATTCACAAACACAGAGAG
PRIMER_LEFT_48=218,23
PRIMER_RIGHT_48=1190,22
PRIMER_LEFT_48_TM=58.373
PRIMER_RIGHT_48_TM=55.732
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=973
PRIMER_PAIR_49_PENALTY=10.908935
PRIMER_LEFT_49_PENALTY=4.641309
PRIMER_RIGHT_49_PENALTY=6.267626
PRIMER_LEFT_49_SEQUENCE=GGAGAATATACGATGGATGAGTGA
PRIMER_RIGHT_49_SEQUENCE=TAGCATTCACAAACACAGAGAG
PRIMER_LEFT_49=219,24
PRIMER_RIGHT_49=1190,22
PRIMER_LEFT_49_TM=59.359
PRIMER_RIGHT_49_TM=55.732
PRIMER_LEFT_49_GC_PERCENT=41.667
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=972
=
SEQUENCE_ID=MAGIC.433_3_13616088_13616349_oy-0_sf-2_347713_278390_TRUE
SEQUENCE_TARGET=950,361
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_2_11041221_11041285_ct-1_zu-0_474652_480688_TRUE
SEQUENCE_TARGET=950,164
SEQUENCE_TEMPLATE=CTCCCATCAGCTGGAGAGCNAAAAGTTAAAATTAATGTTCGAAATTTANTTCATAAAAGCTACGTACGTTGCATCCTTCCATGATCTAGAAAAANTGGGGGACAATAACCAAATTTATTCACTCTTGTCAGGTCATGGGGNGTAGTCTNCGACCTTGAAAGTCACGTGACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATCTGCAGANTTGCGCTTATTAATTGTTTACCGTACGGTATTGTGGATAGGGTCAAGATTTTCATAACTTTACCGTAGTTAATATTTGACCCAACCATGGTTAAAATAAAGNAGTTTATANCACAGANACACACNTCTCTNTNNTCTAGACTCACTNAACAAAAATGATGATCGGCGAAAATAAAAACCGGCCACATCCAACGATCCATATCCCTCAATGGGATCAAATCAACGATCCAACGGCCACNATCTCTTCACCATTCTCTTCCGTCAACCTTAACNGCGTTAACGACTACCCACACTCTCCGTCACCGTATCTCGACTCCTTCGCTTCTCTCTTCCGTTACCTCCCGTCAAACGAGTTAACAAACGATTCAGACTCATCAAGNGGCGACGAGTCATCACCACTCACCGACTCATTCTCCTCCGACGAGTTTCGCATCTACGAGTTCAAAATCCGGCGATGCGCTCGAGGNCGATCTCATGATTGGACGGAGTGTCCGTTCGCACATCCCGGAGAAAAAGCTCGACGACGTGATCCGAGAAAGTTTCATTACTCCGGCACCGCTTGTCCTGAGTTTCGTAAAGGNAGTTGTAGAAGAGGTGATTCNTGTGAGTTCTCTCATGGAGTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_19304459_19304889_tsu-0_rsch-4_427003_223217_TRUE
SEQUENCE_TARGET=950,530
SEQUENCE_TEMPLATE=TCAACAGCTTCCTCAGAGTCATATGAAATGAACCCAAAGCCTCTAGGCCGCTGGGTTCTGTGGTCATACATCACCACAACATCAGTGATCATNCCAAACTGAGCAAANTACTTTTTGAACTCAGCCTCTGTCACGGATGATGCCAAACCTCCCACAAAGATCTTCTTGGAGTTTGATGGGCCAGGTGATCCCTGAAGGCTGCTGTTACTTTTATTAAATACTACGTGATCATCTCTTGGAACNGCCTTCTTTGCCTCAACCTGTATATCATGGAGAAAATNAACACACNACACAACTCAAAGAAAGCAGTTTTTCATTCATTGTGAACCACTTTCTTTCACATGAGGATATGAAACGATCCAAGTTTTACATNTTTATTTGTGAGTGTGAAATGAACAACTTCTGACAAAGATATGCATTTATCAGAGCGGCAAAGCTGATTGTAAACAATAGAGTCTACTAACTAAAACAGGAAGGAATAAAAGTGAGTAGCTTTGCTTTGCTTTGAGAAGCAGCAACCTCAAAATGAAGCAAGTAAGCAGNAAGATTGTTGAAATTGGAAGCAATGTATGTTGGTATNTAGCAGGAGGAAACTTACAATTTTACCATCAATGATATGTTTAAGCAAGACGACTCTTTCAGCAACATTAGGATCAGCAAAGACAACGAAACCAAAGCCACGAGCACGGCCAGTGGCACGATCCTTCATAATAACAGCCTCTAAAACCTCACCAAAACTGTGAAAATAGTCACGAAGACGATCTTCACTGGTTTCCCAAGATATACCACCGATGAAGAGCTTACATGATTCCATTTCCATTTCTGCCAAACAAACAAAAAAANGTTNGTTTTTGNTTCCAGATTCTATATCGATCTAANTGAGAAGGAGAATGGAATCGATAGAAACACCTCAAGGGAAGCAAAATAGGATGAAAAAGAGCAGATGGGTCGTGATCGGATTGTGTAAAAACCGATCGACCCACGATCTTGGATTCCTNCANAAACNNNAANNNNNNNAAATGATCAANAAAGGTGTAAGCTTNNNNGAAGGAAGGAATGAAAANCCCAGAAGACAGAGAATCTATGTATGTGTGTAATACCGAAAATAAAGAAACGGAGAAAGAGGAAAAAAAAAAGATTTTAGGATTGAGAAATCTTCAGATTTGGAGAAGGCAAGGTTTTAATAAGAGGGCGAGAGGCCCTCCTCTCTCTTTTCTGCTTATCGTCCAAATTTTTAGTCTTTTCTTTTCTTTTCTTTTTCTGCAATCCAAATCGAATTAAAAGGAACAAAAATGGATGGAAGCAAATAAGCAATGCTCAGCACAAATTTCAGAAAATGGAAAAAAAAACACTCACAAAAAAGAATACTTTCTCTTACNTTTTTGTTTTTATATGGCAATGTTTGGCTATCCTTGTAAGAGACGAGGGTANTTTCGTCACTTNCGTTTCTTCTCTGTAATAAGTAATTAATTTATTAATAACCAATGCGTGCACCTTTTTANNCATAAGTTAAAGAATAAAATCTATCGTTAATAAAAAATGGCTAATTGGCTTAAGTTGATCATGATTTCTTCCTAGTTCTAGATTTTAGTTGATCATCATAACTTTAATTNGAAGAGAGGTCTATGTTTTTTAAATGGTTCGTTAATCAGATGATTATGAAAGATGNAACATGTGACAATTANNTGATTGCATGAAATTTTAAGATTAAAATGAAAATGGCATGAAACTCTAATTAGAGCTTTTGGGGAATCAAAGTGGGCAGGAACATGGGGAATGGTTGATGACGACGATAAAGATTGCCATCCCACANTTTTTTNTTTANTTATGTCCCTTCATTTTGCTTTCAAATAAACTAATCAAATTTGTTATGAATTGAATAATCATTTTTAAAATCATTCATCAACCAAGTGGAAGATGTTTACAAGTACACAAGGCATTACTTGTGCATCCAAATTATTATTACCAACTTTNATAAGCTTACTTTTCTTCTTTANATTAATCACTTTCTTGCCGTCCAATTATTTTANTTGGNTTTTTTTACACTCTTTAGAAAACAAAAACATCATGTTATGTCGGTTTTATCCTATTAGAAAACACATCGACAACATTTAACCACTAAATTANNNNNANATNTATATATNNNNANTTACAACTATTGATCGTTTTTGTATTNTTCTTTACAAGTCAAATTTCCACCGTTGAAATTTTGATTTACAACATTAATCCAGAGTTTAAAAATTCGGATCATCTAATTCATACATAAGTGAATTAATTAATTAAAAGCGTGACATCAAACGTTAGACACATGTGGAGATGANATTGAATAATGAATATCATCCACTAACCATTNTAGGTTAGAGTCTTGTAGCNTATGTACCAACTATCTCTTTTATACTATTTAAGTNTAACTCTCATCTTGCAAGAATC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.671255
PRIMER_LEFT_0_PENALTY=0.232299
PRIMER_RIGHT_0_PENALTY=0.438956
PRIMER_LEFT_0_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_0_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_0=902,20
PRIMER_RIGHT_0=1785,20
PRIMER_LEFT_0_TM=60.232
PRIMER_RIGHT_0_TM=60.439
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=884
PRIMER_PAIR_1_PENALTY=0.727472
PRIMER_LEFT_1_PENALTY=0.232299
PRIMER_RIGHT_1_PENALTY=0.495173
PRIMER_LEFT_1_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_1_SEQUENCE=TGTTCCTGCCCACTTTGATT
PRIMER_LEFT_1=902,20
PRIMER_RIGHT_1=1769,20
PRIMER_LEFT_1_TM=60.232
PRIMER_RIGHT_1_TM=60.495
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=868
PRIMER_PAIR_2_PENALTY=0.727760
PRIMER_LEFT_2_PENALTY=0.288804
PRIMER_RIGHT_2_PENALTY=0.438956
PRIMER_LEFT_2_SEQUENCE=ACACCTCAAGGGAAGCAAAA
PRIMER_RIGHT_2_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_2=905,20
PRIMER_RIGHT_2=1785,20
PRIMER_LEFT_2_TM=59.711
PRIMER_RIGHT_2_TM=60.439
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=881
PRIMER_PAIR_3_PENALTY=0.727760
PRIMER_LEFT_3_PENALTY=0.288804
PRIMER_RIGHT_3_PENALTY=0.438956
PRIMER_LEFT_3_SEQUENCE=AACACCTCAAGGGAAGCAAA
PRIMER_RIGHT_3_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_3=904,20
PRIMER_RIGHT_3=1785,20
PRIMER_LEFT_3_TM=59.711
PRIMER_RIGHT_3_TM=60.439
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=882
PRIMER_PAIR_4_PENALTY=0.727760
PRIMER_LEFT_4_PENALTY=0.288804
PRIMER_RIGHT_4_PENALTY=0.438956
PRIMER_LEFT_4_SEQUENCE=AAACACCTCAAGGGAAGCAA
PRIMER_RIGHT_4_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_4=903,20
PRIMER_RIGHT_4=1785,20
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=60.439
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=883
PRIMER_PAIR_5_PENALTY=0.768219
PRIMER_LEFT_5_PENALTY=0.232299
PRIMER_RIGHT_5_PENALTY=0.535919
PRIMER_LEFT_5_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_5_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_5=902,20
PRIMER_RIGHT_5=1784,20
PRIMER_LEFT_5_TM=60.232
PRIMER_RIGHT_5_TM=59.464
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=883
PRIMER_PAIR_6_PENALTY=0.824723
PRIMER_LEFT_6_PENALTY=0.288804
PRIMER_RIGHT_6_PENALTY=0.535919
PRIMER_LEFT_6_SEQUENCE=ACACCTCAAGGGAAGCAAAA
PRIMER_RIGHT_6_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_6=905,20
PRIMER_RIGHT_6=1784,20
PRIMER_LEFT_6_TM=59.711
PRIMER_RIGHT_6_TM=59.464
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=880
PRIMER_PAIR_7_PENALTY=0.824723
PRIMER_LEFT_7_PENALTY=0.288804
PRIMER_RIGHT_7_PENALTY=0.535919
PRIMER_LEFT_7_SEQUENCE=AACACCTCAAGGGAAGCAAA
PRIMER_RIGHT_7_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_7=904,20
PRIMER_RIGHT_7=1784,20
PRIMER_LEFT_7_TM=59.711
PRIMER_RIGHT_7_TM=59.464
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.8000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=881
PRIMER_PAIR_8_PENALTY=0.824723
PRIMER_LEFT_8_PENALTY=0.288804
PRIMER_RIGHT_8_PENALTY=0.535919
PRIMER_LEFT_8_SEQUENCE=AAACACCTCAAGGGAAGCAA
PRIMER_RIGHT_8_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_8=903,20
PRIMER_RIGHT_8=1784,20
PRIMER_LEFT_8_TM=59.711
PRIMER_RIGHT_8_TM=59.464
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=882
PRIMER_PAIR_9_PENALTY=0.934233
PRIMER_LEFT_9_PENALTY=0.439061
PRIMER_RIGHT_9_PENALTY=0.495173
PRIMER_LEFT_9_SEQUENCE=CCATTTCTGCCAAACAAACA
PRIMER_RIGHT_9_SEQUENCE=TGTTCCTGCCCACTTTGATT
PRIMER_LEFT_9=816,20
PRIMER_RIGHT_9=1769,20
PRIMER_LEFT_9_TM=59.561
PRIMER_RIGHT_9_TM=60.495
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=954
PRIMER_PAIR_10_PENALTY=0.974980
PRIMER_LEFT_10_PENALTY=0.439061
PRIMER_RIGHT_10_PENALTY=0.535919
PRIMER_LEFT_10_SEQUENCE=CCATTTCTGCCAAACAAACA
PRIMER_RIGHT_10_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_10=816,20
PRIMER_RIGHT_10=1784,20
PRIMER_LEFT_10_TM=59.561
PRIMER_RIGHT_10_TM=59.464
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=969
PRIMER_PAIR_11_PENALTY=1.037931
PRIMER_LEFT_11_PENALTY=0.439061
PRIMER_RIGHT_11_PENALTY=0.598870
PRIMER_LEFT_11_SEQUENCE=CCATTTCTGCCAAACAAACA
PRIMER_RIGHT_11_SEQUENCE=CCACTTTGATTCCCCAAAAG
PRIMER_LEFT_11=816,20
PRIMER_RIGHT_11=1760,20
PRIMER_LEFT_11_TM=59.561
PRIMER_RIGHT_11_TM=59.401
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=945
PRIMER_PAIR_12_PENALTY=1.372692
PRIMER_LEFT_12_PENALTY=0.232299
PRIMER_RIGHT_12_PENALTY=1.140393
PRIMER_LEFT_12_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_12_SEQUENCE=GGGATGGCAATCTTTATCGT
PRIMER_LEFT_12=902,20
PRIMER_RIGHT_12=1808,20
PRIMER_LEFT_12_TM=60.232
PRIMER_RIGHT_12_TM=58.860
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=907
PRIMER_PAIR_13_PENALTY=1.429197
PRIMER_LEFT_13_PENALTY=0.288804
PRIMER_RIGHT_13_PENALTY=1.140393
PRIMER_LEFT_13_SEQUENCE=ACACCTCAAGGGAAGCAAAA
PRIMER_RIGHT_13_SEQUENCE=GGGATGGCAATCTTTATCGT
PRIMER_LEFT_13=905,20
PRIMER_RIGHT_13=1808,20
PRIMER_LEFT_13_TM=59.711
PRIMER_RIGHT_13_TM=58.860
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=8.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=904
PRIMER_PAIR_14_PENALTY=1.429197
PRIMER_LEFT_14_PENALTY=0.288804
PRIMER_RIGHT_14_PENALTY=1.140393
PRIMER_LEFT_14_SEQUENCE=AACACCTCAAGGGAAGCAAA
PRIMER_RIGHT_14_SEQUENCE=GGGATGGCAATCTTTATCGT
PRIMER_LEFT_14=904,20
PRIMER_RIGHT_14=1808,20
PRIMER_LEFT_14_TM=59.711
PRIMER_RIGHT_14_TM=58.860
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.8000
PRIMER_RIGHT_14_END_STABILITY=8.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=905
PRIMER_PAIR_15_PENALTY=1.429197
PRIMER_LEFT_15_PENALTY=0.288804
PRIMER_RIGHT_15_PENALTY=1.140393
PRIMER_LEFT_15_SEQUENCE=AAACACCTCAAGGGAAGCAA
PRIMER_RIGHT_15_SEQUENCE=GGGATGGCAATCTTTATCGT
PRIMER_LEFT_15=903,20
PRIMER_RIGHT_15=1808,20
PRIMER_LEFT_15_TM=59.711
PRIMER_RIGHT_15_TM=58.860
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=906
PRIMER_PAIR_16_PENALTY=1.456079
PRIMER_LEFT_16_PENALTY=0.232299
PRIMER_RIGHT_16_PENALTY=1.223779
PRIMER_LEFT_16_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_16_SEQUENCE=TGTGGGATGGCAATCTTTATC
PRIMER_LEFT_16=902,20
PRIMER_RIGHT_16=1811,21
PRIMER_LEFT_16_TM=60.232
PRIMER_RIGHT_16_TM=59.776
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=5.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=910
PRIMER_PAIR_17_PENALTY=1.508928
PRIMER_LEFT_17_PENALTY=0.232299
PRIMER_RIGHT_17_PENALTY=1.276629
PRIMER_LEFT_17_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_17_SEQUENCE=GGCAATCTTTATCGTCGTCA
PRIMER_LEFT_17=902,20
PRIMER_RIGHT_17=1803,20
PRIMER_LEFT_17_TM=60.232
PRIMER_RIGHT_17_TM=58.723
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=902
PRIMER_PAIR_18_PENALTY=1.512583
PRIMER_LEFT_18_PENALTY=0.288804
PRIMER_RIGHT_18_PENALTY=1.223779
PRIMER_LEFT_18_SEQUENCE=ACACCTCAAGGGAAGCAAAA
PRIMER_RIGHT_18_SEQUENCE=TGTGGGATGGCAATCTTTATC
PRIMER_LEFT_18=905,20
PRIMER_RIGHT_18=1811,21
PRIMER_LEFT_18_TM=59.711
PRIMER_RIGHT_18_TM=59.776
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=907
PRIMER_PAIR_19_PENALTY=1.512583
PRIMER_LEFT_19_PENALTY=0.288804
PRIMER_RIGHT_19_PENALTY=1.223779
PRIMER_LEFT_19_SEQUENCE=AACACCTCAAGGGAAGCAAA
PRIMER_RIGHT_19_SEQUENCE=TGTGGGATGGCAATCTTTATC
PRIMER_LEFT_19=904,20
PRIMER_RIGHT_19=1811,21
PRIMER_LEFT_19_TM=59.711
PRIMER_RIGHT_19_TM=59.776
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.8000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=908
PRIMER_PAIR_20_PENALTY=1.512583
PRIMER_LEFT_20_PENALTY=0.288804
PRIMER_RIGHT_20_PENALTY=1.223779
PRIMER_LEFT_20_SEQUENCE=AAACACCTCAAGGGAAGCAA
PRIMER_RIGHT_20_SEQUENCE=TGTGGGATGGCAATCTTTATC
PRIMER_LEFT_20=903,20
PRIMER_RIGHT_20=1811,21
PRIMER_LEFT_20_TM=59.711
PRIMER_RIGHT_20_TM=59.776
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=5.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=909
PRIMER_PAIR_21_PENALTY=1.565433
PRIMER_LEFT_21_PENALTY=0.288804
PRIMER_RIGHT_21_PENALTY=1.276629
PRIMER_LEFT_21_SEQUENCE=ACACCTCAAGGGAAGCAAAA
PRIMER_RIGHT_21_SEQUENCE=GGCAATCTTTATCGTCGTCA
PRIMER_LEFT_21=905,20
PRIMER_RIGHT_21=1803,20
PRIMER_LEFT_21_TM=59.711
PRIMER_RIGHT_21_TM=58.723
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=899
PRIMER_PAIR_22_PENALTY=1.565433
PRIMER_LEFT_22_PENALTY=0.288804
PRIMER_RIGHT_22_PENALTY=1.276629
PRIMER_LEFT_22_SEQUENCE=AACACCTCAAGGGAAGCAAA
PRIMER_RIGHT_22_SEQUENCE=GGCAATCTTTATCGTCGTCA
PRIMER_LEFT_22=904,20
PRIMER_RIGHT_22=1803,20
PRIMER_LEFT_22_TM=59.711
PRIMER_RIGHT_22_TM=58.723
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.8000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=900
PRIMER_PAIR_23_PENALTY=1.565433
PRIMER_LEFT_23_PENALTY=0.288804
PRIMER_RIGHT_23_PENALTY=1.276629
PRIMER_LEFT_23_SEQUENCE=AAACACCTCAAGGGAAGCAA
PRIMER_RIGHT_23_SEQUENCE=GGCAATCTTTATCGTCGTCA
PRIMER_LEFT_23=903,20
PRIMER_RIGHT_23=1803,20
PRIMER_LEFT_23_TM=59.711
PRIMER_RIGHT_23_TM=58.723
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=901
PRIMER_PAIR_24_PENALTY=1.579454
PRIMER_LEFT_24_PENALTY=0.439061
PRIMER_RIGHT_24_PENALTY=1.140393
PRIMER_LEFT_24_SEQUENCE=CCATTTCTGCCAAACAAACA
PRIMER_RIGHT_24_SEQUENCE=GGGATGGCAATCTTTATCGT
PRIMER_LEFT_24=816,20
PRIMER_RIGHT_24=1808,20
PRIMER_LEFT_24_TM=59.561
PRIMER_RIGHT_24_TM=58.860
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.0000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=993
PRIMER_PAIR_25_PENALTY=1.604132
PRIMER_LEFT_25_PENALTY=1.165177
PRIMER_RIGHT_25_PENALTY=0.438956
PRIMER_LEFT_25_SEQUENCE=ATTCCATTTCCATTTCTGCC
PRIMER_RIGHT_25_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_25=807,20
PRIMER_RIGHT_25=1785,20
PRIMER_LEFT_25_TM=58.835
PRIMER_RIGHT_25_TM=60.439
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=9.7000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=979
PRIMER_PAIR_26_PENALTY=1.611054
PRIMER_LEFT_26_PENALTY=1.075135
PRIMER_RIGHT_26_PENALTY=0.535919
PRIMER_LEFT_26_SEQUENCE=CCTCAAGGGAAGCAAAATAGG
PRIMER_RIGHT_26_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_26=908,21
PRIMER_RIGHT_26=1784,20
PRIMER_LEFT_26_TM=60.075
PRIMER_RIGHT_26_TM=59.464
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.1000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=877
PRIMER_PAIR_27_PENALTY=1.660349
PRIMER_LEFT_27_PENALTY=1.165177
PRIMER_RIGHT_27_PENALTY=0.495173
PRIMER_LEFT_27_SEQUENCE=ATTCCATTTCCATTTCTGCC
PRIMER_RIGHT_27_SEQUENCE=TGTTCCTGCCCACTTTGATT
PRIMER_LEFT_27=807,20
PRIMER_RIGHT_27=1769,20
PRIMER_LEFT_27_TM=58.835
PRIMER_RIGHT_27_TM=60.495
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=963
PRIMER_PAIR_28_PENALTY=1.662840
PRIMER_LEFT_28_PENALTY=0.439061
PRIMER_RIGHT_28_PENALTY=1.223779
PRIMER_LEFT_28_SEQUENCE=CCATTTCTGCCAAACAAACA
PRIMER_RIGHT_28_SEQUENCE=TGTGGGATGGCAATCTTTATC
PRIMER_LEFT_28=816,20
PRIMER_RIGHT_28=1811,21
PRIMER_LEFT_28_TM=59.561
PRIMER_RIGHT_28_TM=59.776
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=5.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=996
PRIMER_PAIR_29_PENALTY=1.701096
PRIMER_LEFT_29_PENALTY=1.165177
PRIMER_RIGHT_29_PENALTY=0.535919
PRIMER_LEFT_29_SEQUENCE=ATTCCATTTCCATTTCTGCC
PRIMER_RIGHT_29_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_29=807,20
PRIMER_RIGHT_29=1784,20
PRIMER_LEFT_29_TM=58.835
PRIMER_RIGHT_29_TM=59.464
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=978
PRIMER_PAIR_30_PENALTY=1.715689
PRIMER_LEFT_30_PENALTY=0.439061
PRIMER_RIGHT_30_PENALTY=1.276629
PRIMER_LEFT_30_SEQUENCE=CCATTTCTGCCAAACAAACA
PRIMER_RIGHT_30_SEQUENCE=GGCAATCTTTATCGTCGTCA
PRIMER_LEFT_30=816,20
PRIMER_RIGHT_30=1803,20
PRIMER_LEFT_30_TM=59.561
PRIMER_RIGHT_30_TM=58.723
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=988
PRIMER_PAIR_31_PENALTY=1.764046
PRIMER_LEFT_31_PENALTY=1.165177
PRIMER_RIGHT_31_PENALTY=0.598870
PRIMER_LEFT_31_SEQUENCE=ATTCCATTTCCATTTCTGCC
PRIMER_RIGHT_31_SEQUENCE=CCACTTTGATTCCCCAAAAG
PRIMER_LEFT_31=807,20
PRIMER_RIGHT_31=1760,20
PRIMER_LEFT_31_TM=58.835
PRIMER_RIGHT_31_TM=59.401
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.7000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=954
PRIMER_PAIR_32_PENALTY=1.783004
PRIMER_LEFT_32_PENALTY=1.344048
PRIMER_RIGHT_32_PENALTY=0.438956
PRIMER_LEFT_32_SEQUENCE=TCCATTTCCATTTCTGCCAA
PRIMER_RIGHT_32_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_32=809,20
PRIMER_RIGHT_32=1785,20
PRIMER_LEFT_32_TM=61.344
PRIMER_RIGHT_32_TM=60.439
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=10.0000
PRIMER_RIGHT_32_END_STABILITY=6.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=977
PRIMER_PAIR_33_PENALTY=1.839221
PRIMER_LEFT_33_PENALTY=1.344048
PRIMER_RIGHT_33_PENALTY=0.495173
PRIMER_LEFT_33_SEQUENCE=TCCATTTCCATTTCTGCCAA
PRIMER_RIGHT_33_SEQUENCE=TGTTCCTGCCCACTTTGATT
PRIMER_LEFT_33=809,20
PRIMER_RIGHT_33=1769,20
PRIMER_LEFT_33_TM=61.344
PRIMER_RIGHT_33_TM=60.495
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=10.0000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=961
PRIMER_PAIR_34_PENALTY=1.840294
PRIMER_LEFT_34_PENALTY=1.401339
PRIMER_RIGHT_34_PENALTY=0.438956
PRIMER_LEFT_34_SEQUENCE=TCCATTTCTGCCAAACAAAC
PRIMER_RIGHT_34_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_34=815,20
PRIMER_RIGHT_34=1785,20
PRIMER_LEFT_34_TM=58.599
PRIMER_RIGHT_34_TM=60.439
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=971
PRIMER_PAIR_35_PENALTY=1.872145
PRIMER_LEFT_35_PENALTY=0.232299
PRIMER_RIGHT_35_PENALTY=1.639846
PRIMER_LEFT_35_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_35_SEQUENCE=ATCGTCGTCATCAACCATTC
PRIMER_LEFT_35=902,20
PRIMER_RIGHT_35=1793,20
PRIMER_LEFT_35_TM=60.232
PRIMER_RIGHT_35_TM=58.360
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=892
PRIMER_PAIR_36_PENALTY=1.879967
PRIMER_LEFT_36_PENALTY=1.344048
PRIMER_RIGHT_36_PENALTY=0.535919
PRIMER_LEFT_36_SEQUENCE=TCCATTTCCATTTCTGCCAA
PRIMER_RIGHT_36_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_36=809,20
PRIMER_RIGHT_36=1784,20
PRIMER_LEFT_36_TM=61.344
PRIMER_RIGHT_36_TM=59.464
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.0000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=976
PRIMER_PAIR_37_PENALTY=1.889175
PRIMER_LEFT_37_PENALTY=0.232299
PRIMER_RIGHT_37_PENALTY=1.656876
PRIMER_LEFT_37_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_37_SEQUENCE=GGGATGGCAATCTTTATCGTC
PRIMER_LEFT_37=902,20
PRIMER_RIGHT_37=1808,21
PRIMER_LEFT_37_TM=60.232
PRIMER_RIGHT_37_TM=60.657
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=8.1000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=907
PRIMER_PAIR_38_PENALTY=1.928650
PRIMER_LEFT_38_PENALTY=0.288804
PRIMER_RIGHT_38_PENALTY=1.639846
PRIMER_LEFT_38_SEQUENCE=ACACCTCAAGGGAAGCAAAA
PRIMER_RIGHT_38_SEQUENCE=ATCGTCGTCATCAACCATTC
PRIMER_LEFT_38=905,20
PRIMER_RIGHT_38=1793,20
PRIMER_LEFT_38_TM=59.711
PRIMER_RIGHT_38_TM=58.360
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=889
PRIMER_PAIR_39_PENALTY=1.928650
PRIMER_LEFT_39_PENALTY=0.288804
PRIMER_RIGHT_39_PENALTY=1.639846
PRIMER_LEFT_39_SEQUENCE=AACACCTCAAGGGAAGCAAA
PRIMER_RIGHT_39_SEQUENCE=ATCGTCGTCATCAACCATTC
PRIMER_LEFT_39=904,20
PRIMER_RIGHT_39=1793,20
PRIMER_LEFT_39_TM=59.711
PRIMER_RIGHT_39_TM=58.360
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.8000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=890
PRIMER_PAIR_40_PENALTY=1.928650
PRIMER_LEFT_40_PENALTY=0.288804
PRIMER_RIGHT_40_PENALTY=1.639846
PRIMER_LEFT_40_SEQUENCE=AAACACCTCAAGGGAAGCAA
PRIMER_RIGHT_40_SEQUENCE=ATCGTCGTCATCAACCATTC
PRIMER_LEFT_40=903,20
PRIMER_RIGHT_40=1793,20
PRIMER_LEFT_40_TM=59.711
PRIMER_RIGHT_40_TM=58.360
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=891
PRIMER_PAIR_41_PENALTY=1.937258
PRIMER_LEFT_41_PENALTY=1.401339
PRIMER_RIGHT_41_PENALTY=0.535919
PRIMER_LEFT_41_SEQUENCE=TCCATTTCTGCCAAACAAAC
PRIMER_RIGHT_41_SEQUENCE=ATCAACCATTCCCCATGTTC
PRIMER_LEFT_41=815,20
PRIMER_RIGHT_41=1784,20
PRIMER_LEFT_41_TM=58.599
PRIMER_RIGHT_41_TM=59.464
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=970
PRIMER_PAIR_42_PENALTY=1.942918
PRIMER_LEFT_42_PENALTY=1.344048
PRIMER_RIGHT_42_PENALTY=0.598870
PRIMER_LEFT_42_SEQUENCE=TCCATTTCCATTTCTGCCAA
PRIMER_RIGHT_42_SEQUENCE=CCACTTTGATTCCCCAAAAG
PRIMER_LEFT_42=809,20
PRIMER_RIGHT_42=1760,20
PRIMER_LEFT_42_TM=61.344
PRIMER_RIGHT_42_TM=59.401
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.0000
PRIMER_RIGHT_42_END_STABILITY=7.3000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=952
PRIMER_PAIR_43_PENALTY=1.945680
PRIMER_LEFT_43_PENALTY=0.288804
PRIMER_RIGHT_43_PENALTY=1.656876
PRIMER_LEFT_43_SEQUENCE=ACACCTCAAGGGAAGCAAAA
PRIMER_RIGHT_43_SEQUENCE=GGGATGGCAATCTTTATCGTC
PRIMER_LEFT_43=905,20
PRIMER_RIGHT_43=1808,21
PRIMER_LEFT_43_TM=59.711
PRIMER_RIGHT_43_TM=60.657
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=8.1000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=904
PRIMER_PAIR_44_PENALTY=1.945680
PRIMER_LEFT_44_PENALTY=0.288804
PRIMER_RIGHT_44_PENALTY=1.656876
PRIMER_LEFT_44_SEQUENCE=AACACCTCAAGGGAAGCAAA
PRIMER_RIGHT_44_SEQUENCE=GGGATGGCAATCTTTATCGTC
PRIMER_LEFT_44=904,20
PRIMER_RIGHT_44=1808,21
PRIMER_LEFT_44_TM=59.711
PRIMER_RIGHT_44_TM=60.657
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.8000
PRIMER_RIGHT_44_END_STABILITY=8.1000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=905
PRIMER_PAIR_45_PENALTY=1.945680
PRIMER_LEFT_45_PENALTY=0.288804
PRIMER_RIGHT_45_PENALTY=1.656876
PRIMER_LEFT_45_SEQUENCE=AAACACCTCAAGGGAAGCAA
PRIMER_RIGHT_45_SEQUENCE=GGGATGGCAATCTTTATCGTC
PRIMER_LEFT_45=903,20
PRIMER_RIGHT_45=1808,21
PRIMER_LEFT_45_TM=59.711
PRIMER_RIGHT_45_TM=60.657
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=8.1000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=906
PRIMER_PAIR_46_PENALTY=1.970161
PRIMER_LEFT_46_PENALTY=0.232299
PRIMER_RIGHT_46_PENALTY=1.737862
PRIMER_LEFT_46_SEQUENCE=GAAACACCTCAAGGGAAGCA
PRIMER_RIGHT_46_SEQUENCE=ATGTTCCTGCCCACTTTGATT
PRIMER_LEFT_46=902,20
PRIMER_RIGHT_46=1770,21
PRIMER_LEFT_46_TM=60.232
PRIMER_RIGHT_46_TM=60.738
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=869
PRIMER_PAIR_47_PENALTY=2.000209
PRIMER_LEFT_47_PENALTY=1.401339
PRIMER_RIGHT_47_PENALTY=0.598870
PRIMER_LEFT_47_SEQUENCE=TCCATTTCTGCCAAACAAAC
PRIMER_RIGHT_47_SEQUENCE=CCACTTTGATTCCCCAAAAG
PRIMER_LEFT_47=815,20
PRIMER_RIGHT_47=1760,20
PRIMER_LEFT_47_TM=58.599
PRIMER_RIGHT_47_TM=59.401
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=946
PRIMER_PAIR_48_PENALTY=2.076088
PRIMER_LEFT_48_PENALTY=1.637132
PRIMER_RIGHT_48_PENALTY=0.438956
PRIMER_LEFT_48_SEQUENCE=GATTCCATTTCCATTTCTGCC
PRIMER_RIGHT_48_SEQUENCE=CATCAACCATTCCCCATGTT
PRIMER_LEFT_48=806,21
PRIMER_RIGHT_48=1785,20
PRIMER_LEFT_48_TM=60.637
PRIMER_RIGHT_48_TM=60.439
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=6.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=980
PRIMER_PAIR_49_PENALTY=2.078906
PRIMER_LEFT_49_PENALTY=0.439061
PRIMER_RIGHT_49_PENALTY=1.639846
PRIMER_LEFT_49_SEQUENCE=CCATTTCTGCCAAACAAACA
PRIMER_RIGHT_49_SEQUENCE=ATCGTCGTCATCAACCATTC
PRIMER_LEFT_49=816,20
PRIMER_RIGHT_49=1793,20
PRIMER_LEFT_49_TM=59.561
PRIMER_RIGHT_49_TM=58.360
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=978
=
SEQUENCE_ID=MAGIC.433_2_6076089_6076169_ws-0_ler-0_2722_408097_TRUE
SEQUENCE_TARGET=950,180
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4232841_4233318_wil-2_edi-0_37276_19081_TRUE
SEQUENCE_TARGET=950,577
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_14397430_14397434_can-0_no-0_20560_41480_TRUE
SEQUENCE_TARGET=950,104
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATAATGAAATGCTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_1640353_1640433_edi-0_bur-0_616318_1440_TRUE
SEQUENCE_TARGET=950,180
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTATGAAANCAATTCGCAACCACTCAATAAATATNNNNNNNNNNNNGTTCGTNGTGCNTNTACCTAANCNTAATTAAAATGAATAAAACCTTGAAANTATCATCGCCTTNCNTTGACTTTAGCTGTCTNGAGACTCTCACATGGTTGNNGTTAGAGCANATCCAACCATTTTAGTTTTACGTGGTGTGNTNTTAATTACATATACAATATCTTAATTAATTAATGATGTCATTTGTCTTTAAGAGGATTTCATGTTGTGTCGTGGNCTTCACGTTGTGTTGGCCGGCCANGTGGTATTAATTTTGTTTATGTATGTATAGCTTNGAGAAATGAGAGAGGAAAAACNAANAAGANAAAAAAAAANTAGAGCAAAAGAGAGATTTTAATCATCATGGAAATAGTGGAGGTANCANCANTTCACNAACATCCTTTGTTACCTNTTACTCGTTTTTCTCGTNAACATNGTNAATGTAANGTNTGTGNNTCNGTTGGTTACATTTACGGAGGCTANTGTTNCAATGAGNTGGNATGTGAATCNATGTTTCATAAAGAGTGTNTTGAGTCTTTGTCAGAAATCAAGCACACTNCTCATCCTGATCATCCTCTCAAGCTGNTNNTAAGTGACAAAGCATCNTTNTGTAGTCGGTGTGAAGGTCGGTTTGAAAANGGTTANANATGTTCTATTTGCGACTTCAAGTTGGANTTTCNTTGTGCTAAANNNNNNNNNNCACTCCTTATTCTTGAAAAATCNAATCTGCACGAGCATCNNCTTGAATTCTTCGTGGGATGGCNCNNTTGGAAGGGANATNGAGAGTGCAAAGCGTGCGGCGGTGCNGGTTTCCNCGGTTNTCNATGGTANGGATGTNATCAATGTGNTACACTCTTCCATGGNGAATGTNNTGAGTNNTTNCCAGAAGCAAAACACACNTCTCACCCGCAACACACACTCAANCACATCACATNTNAAGAAGCACCCGATTACGCCGACAANAAGTGTCTTCTTTGCGAGGAGGAGTTNGATCNACAACATCCCAAAATTCACCATTGTGATGTTTGTAATTTCANCATATGTAGANCATGTATGAAAANCGCACCACCNGTTCGTNTTGAAAGCCNTACNACCCATGAACATCANCTNCATCTCGTTCCAAGACGCATTGATTTCACTTGCAATGCTTGNGGGACGCTAGGTGACCGAAGTCCTTANTTNTGNCNNNNNNNNNNNNNNNNNNTTCATCGNCAGTGTATCGATTTACCNCGNGTCATAAACATCAACCGCCACNATCACCGCATCTCTTACACCCGTCGCCTTGGACATGGAGANTGGAAATGNAAANTCTGCCGCAAAAAAGTNGATGGGTTCTANGGGGCTTATACTTGCCTCAAATGTCCTAGTTTTGTTGTTCATTCACTATGNGCAACAAGAAANGATGTGTGGGACATGATAGAGCTAGAAGGGATGCNNGAAGAAGAGGAAATTGCNCCATACGAGATGATTGNTGCTAACACGATAAAACATTTCAGCCATGATCATAACNTACAAATCAACAATGATGGNCATNGCNGNATTCAACCTGAAAATATAGTTTGTGAAGCNTGTGTNTTCCAGATNTTNTCAGAACCGTTCTACAGTTGC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.222618
PRIMER_LEFT_0_PENALTY=2.212073
PRIMER_RIGHT_0_PENALTY=0.010545
PRIMER_LEFT_0_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_0_SEQUENCE=CCTCCTCGCAAAGAAGACAC
PRIMER_LEFT_0=688,21
PRIMER_RIGHT_0=1456,20
PRIMER_LEFT_0_TM=61.212
PRIMER_RIGHT_0_TM=59.989
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=769
PRIMER_PAIR_1_PENALTY=2.672137
PRIMER_LEFT_1_PENALTY=2.661591
PRIMER_RIGHT_1_PENALTY=0.010545
PRIMER_LEFT_1_SEQUENCE=GATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_1_SEQUENCE=CCTCCTCGCAAAGAAGACAC
PRIMER_LEFT_1=689,20
PRIMER_RIGHT_1=1456,20
PRIMER_LEFT_1_TM=57.338
PRIMER_RIGHT_1_TM=59.989
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=768
PRIMER_PAIR_2_PENALTY=2.931304
PRIMER_LEFT_2_PENALTY=2.212073
PRIMER_RIGHT_2_PENALTY=0.719232
PRIMER_LEFT_2_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_2_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_2=688,21
PRIMER_RIGHT_2=1614,20
PRIMER_LEFT_2_TM=61.212
PRIMER_RIGHT_2_TM=59.281
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=927
PRIMER_PAIR_3_PENALTY=2.990339
PRIMER_LEFT_3_PENALTY=2.212073
PRIMER_RIGHT_3_PENALTY=0.778266
PRIMER_LEFT_3_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_3_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_3=688,21
PRIMER_RIGHT_3=1487,20
PRIMER_LEFT_3_TM=61.212
PRIMER_RIGHT_3_TM=59.222
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=800
PRIMER_PAIR_4_PENALTY=2.990339
PRIMER_LEFT_4_PENALTY=2.212073
PRIMER_RIGHT_4_PENALTY=0.778266
PRIMER_LEFT_4_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_4_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_4=688,21
PRIMER_RIGHT_4=1492,20
PRIMER_LEFT_4_TM=61.212
PRIMER_RIGHT_4_TM=59.222
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=805
PRIMER_PAIR_5_PENALTY=3.137917
PRIMER_LEFT_5_PENALTY=2.418685
PRIMER_RIGHT_5_PENALTY=0.719232
PRIMER_LEFT_5_SEQUENCE=GAGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_5_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_5=686,22
PRIMER_RIGHT_5=1614,20
PRIMER_LEFT_5_TM=60.419
PRIMER_RIGHT_5_TM=59.281
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=929
PRIMER_PAIR_6_PENALTY=3.196952
PRIMER_LEFT_6_PENALTY=2.418685
PRIMER_RIGHT_6_PENALTY=0.778266
PRIMER_LEFT_6_SEQUENCE=GAGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_6_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_6=686,22
PRIMER_RIGHT_6=1487,20
PRIMER_LEFT_6_TM=60.419
PRIMER_RIGHT_6_TM=59.222
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=802
PRIMER_PAIR_7_PENALTY=3.196952
PRIMER_LEFT_7_PENALTY=2.418685
PRIMER_RIGHT_7_PENALTY=0.778266
PRIMER_LEFT_7_SEQUENCE=GAGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_7_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_7=686,22
PRIMER_RIGHT_7=1492,20
PRIMER_LEFT_7_TM=60.419
PRIMER_RIGHT_7_TM=59.222
PRIMER_LEFT_7_GC_PERCENT=45.455
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.4000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=807
PRIMER_PAIR_8_PENALTY=3.235535
PRIMER_LEFT_8_PENALTY=2.516303
PRIMER_RIGHT_8_PENALTY=0.719232
PRIMER_LEFT_8_SEQUENCE=AGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_8_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_8=687,21
PRIMER_RIGHT_8=1614,20
PRIMER_LEFT_8_TM=58.484
PRIMER_RIGHT_8_TM=59.281
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=7.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=928
PRIMER_PAIR_9_PENALTY=3.294569
PRIMER_LEFT_9_PENALTY=2.516303
PRIMER_RIGHT_9_PENALTY=0.778266
PRIMER_LEFT_9_SEQUENCE=AGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_9_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_9=687,21
PRIMER_RIGHT_9=1487,20
PRIMER_LEFT_9_TM=58.484
PRIMER_RIGHT_9_TM=59.222
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=801
PRIMER_PAIR_10_PENALTY=3.294569
PRIMER_LEFT_10_PENALTY=2.516303
PRIMER_RIGHT_10_PENALTY=0.778266
PRIMER_LEFT_10_SEQUENCE=AGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_10_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_10=687,21
PRIMER_RIGHT_10=1492,20
PRIMER_LEFT_10_TM=58.484
PRIMER_RIGHT_10_TM=59.222
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=806
PRIMER_PAIR_11_PENALTY=3.380823
PRIMER_LEFT_11_PENALTY=2.661591
PRIMER_RIGHT_11_PENALTY=0.719232
PRIMER_LEFT_11_SEQUENCE=GATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_11_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_11=689,20
PRIMER_RIGHT_11=1614,20
PRIMER_LEFT_11_TM=57.338
PRIMER_RIGHT_11_TM=59.281
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.9000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=7.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=926
PRIMER_PAIR_12_PENALTY=3.380823
PRIMER_LEFT_12_PENALTY=2.661591
PRIMER_RIGHT_12_PENALTY=0.719232
PRIMER_LEFT_12_SEQUENCE=GGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_12_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_12=688,20
PRIMER_RIGHT_12=1614,20
PRIMER_LEFT_12_TM=57.338
PRIMER_RIGHT_12_TM=59.281
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=927
PRIMER_PAIR_13_PENALTY=3.439858
PRIMER_LEFT_13_PENALTY=2.661591
PRIMER_RIGHT_13_PENALTY=0.778266
PRIMER_LEFT_13_SEQUENCE=GATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_13_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_13=689,20
PRIMER_RIGHT_13=1487,20
PRIMER_LEFT_13_TM=57.338
PRIMER_RIGHT_13_TM=59.222
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=799
PRIMER_PAIR_14_PENALTY=3.439858
PRIMER_LEFT_14_PENALTY=2.661591
PRIMER_RIGHT_14_PENALTY=0.778266
PRIMER_LEFT_14_SEQUENCE=GATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_14_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_14=689,20
PRIMER_RIGHT_14=1492,20
PRIMER_LEFT_14_TM=57.338
PRIMER_RIGHT_14_TM=59.222
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=804
PRIMER_PAIR_15_PENALTY=3.439858
PRIMER_LEFT_15_PENALTY=2.661591
PRIMER_RIGHT_15_PENALTY=0.778266
PRIMER_LEFT_15_SEQUENCE=GGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_15_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_15=688,20
PRIMER_RIGHT_15=1487,20
PRIMER_LEFT_15_TM=57.338
PRIMER_RIGHT_15_TM=59.222
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=800
PRIMER_PAIR_16_PENALTY=3.439858
PRIMER_LEFT_16_PENALTY=2.661591
PRIMER_RIGHT_16_PENALTY=0.778266
PRIMER_LEFT_16_SEQUENCE=GGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_16_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_16=688,20
PRIMER_RIGHT_16=1492,20
PRIMER_LEFT_16_TM=57.338
PRIMER_RIGHT_16_TM=59.222
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=805
PRIMER_PAIR_17_PENALTY=3.483669
PRIMER_LEFT_17_PENALTY=2.212073
PRIMER_RIGHT_17_PENALTY=1.271596
PRIMER_LEFT_17_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_17_SEQUENCE=GCAAGTGAAATCAATGCGTCT
PRIMER_LEFT_17=688,21
PRIMER_RIGHT_17=1614,21
PRIMER_LEFT_17_TM=61.212
PRIMER_RIGHT_17_TM=60.272
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=8.1000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=927
PRIMER_PAIR_18_PENALTY=3.538398
PRIMER_LEFT_18_PENALTY=3.527853
PRIMER_RIGHT_18_PENALTY=0.010545
PRIMER_LEFT_18_SEQUENCE=TCATCATGGAAATAGTGGAGG
PRIMER_RIGHT_18_SEQUENCE=CCTCCTCGCAAAGAAGACAC
PRIMER_LEFT_18=830,21
PRIMER_RIGHT_18=1456,20
PRIMER_LEFT_18_TM=57.472
PRIMER_RIGHT_18_TM=59.989
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=627
PRIMER_PAIR_19_PENALTY=3.543153
PRIMER_LEFT_19_PENALTY=2.212073
PRIMER_RIGHT_19_PENALTY=1.331080
PRIMER_LEFT_19_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_19_SEQUENCE=CAATGGTGAATTTTGGGATG
PRIMER_LEFT_19=688,21
PRIMER_RIGHT_19=1490,20
PRIMER_LEFT_19_TM=61.212
PRIMER_RIGHT_19_TM=58.669
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=803
PRIMER_PAIR_20_PENALTY=3.625000
PRIMER_LEFT_20_PENALTY=3.614455
PRIMER_RIGHT_20_PENALTY=0.010545
PRIMER_LEFT_20_SEQUENCE=TCATCATGGAAATAGTGGAGGT
PRIMER_RIGHT_20_SEQUENCE=CCTCCTCGCAAAGAAGACAC
PRIMER_LEFT_20=830,22
PRIMER_RIGHT_20=1456,20
PRIMER_LEFT_20_TM=58.386
PRIMER_RIGHT_20_TM=59.989
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=6.1000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=627
PRIMER_PAIR_21_PENALTY=3.646779
PRIMER_LEFT_21_PENALTY=2.212073
PRIMER_RIGHT_21_PENALTY=1.434707
PRIMER_LEFT_21_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_21_SEQUENCE=GAAATCAATGCGTCTTGGAAC
PRIMER_LEFT_21=688,21
PRIMER_RIGHT_21=1608,21
PRIMER_LEFT_21_TM=61.212
PRIMER_RIGHT_21_TM=59.565
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=921
PRIMER_PAIR_22_PENALTY=3.690282
PRIMER_LEFT_22_PENALTY=2.418685
PRIMER_RIGHT_22_PENALTY=1.271596
PRIMER_LEFT_22_SEQUENCE=GAGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_22_SEQUENCE=GCAAGTGAAATCAATGCGTCT
PRIMER_LEFT_22=686,22
PRIMER_RIGHT_22=1614,21
PRIMER_LEFT_22_TM=60.419
PRIMER_RIGHT_22_TM=60.272
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.4000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=929
PRIMER_PAIR_23_PENALTY=3.749765
PRIMER_LEFT_23_PENALTY=2.418685
PRIMER_RIGHT_23_PENALTY=1.331080
PRIMER_LEFT_23_SEQUENCE=GAGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_23_SEQUENCE=CAATGGTGAATTTTGGGATG
PRIMER_LEFT_23=686,22
PRIMER_RIGHT_23=1490,20
PRIMER_LEFT_23_TM=60.419
PRIMER_RIGHT_23_TM=58.669
PRIMER_LEFT_23_GC_PERCENT=45.455
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=8.1000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=805
PRIMER_PAIR_24_PENALTY=3.787899
PRIMER_LEFT_24_PENALTY=2.516303
PRIMER_RIGHT_24_PENALTY=1.271596
PRIMER_LEFT_24_SEQUENCE=AGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_24_SEQUENCE=GCAAGTGAAATCAATGCGTCT
PRIMER_LEFT_24=687,21
PRIMER_RIGHT_24=1614,21
PRIMER_LEFT_24_TM=58.484
PRIMER_RIGHT_24_TM=60.272
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=7.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=928
PRIMER_PAIR_25_PENALTY=3.847383
PRIMER_LEFT_25_PENALTY=2.516303
PRIMER_RIGHT_25_PENALTY=1.331080
PRIMER_LEFT_25_SEQUENCE=AGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_25_SEQUENCE=CAATGGTGAATTTTGGGATG
PRIMER_LEFT_25=687,21
PRIMER_RIGHT_25=1490,20
PRIMER_LEFT_25_TM=58.484
PRIMER_RIGHT_25_TM=58.669
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=8.1000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=804
PRIMER_PAIR_26_PENALTY=3.853392
PRIMER_LEFT_26_PENALTY=2.418685
PRIMER_RIGHT_26_PENALTY=1.434707
PRIMER_LEFT_26_SEQUENCE=GAGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_26_SEQUENCE=GAAATCAATGCGTCTTGGAAC
PRIMER_LEFT_26=686,22
PRIMER_RIGHT_26=1608,21
PRIMER_LEFT_26_TM=60.419
PRIMER_RIGHT_26_TM=59.565
PRIMER_LEFT_26_GC_PERCENT=45.455
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=923
PRIMER_PAIR_27_PENALTY=3.933188
PRIMER_LEFT_27_PENALTY=2.661591
PRIMER_RIGHT_27_PENALTY=1.271596
PRIMER_LEFT_27_SEQUENCE=GATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_27_SEQUENCE=GCAAGTGAAATCAATGCGTCT
PRIMER_LEFT_27=689,20
PRIMER_RIGHT_27=1614,21
PRIMER_LEFT_27_TM=57.338
PRIMER_RIGHT_27_TM=60.272
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=8.1000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=926
PRIMER_PAIR_28_PENALTY=3.933188
PRIMER_LEFT_28_PENALTY=2.661591
PRIMER_RIGHT_28_PENALTY=1.271596
PRIMER_LEFT_28_SEQUENCE=GGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_28_SEQUENCE=GCAAGTGAAATCAATGCGTCT
PRIMER_LEFT_28=688,20
PRIMER_RIGHT_28=1614,21
PRIMER_LEFT_28_TM=57.338
PRIMER_RIGHT_28_TM=60.272
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=927
PRIMER_PAIR_29_PENALTY=3.951010
PRIMER_LEFT_29_PENALTY=2.516303
PRIMER_RIGHT_29_PENALTY=1.434707
PRIMER_LEFT_29_SEQUENCE=AGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_29_SEQUENCE=GAAATCAATGCGTCTTGGAAC
PRIMER_LEFT_29=687,21
PRIMER_RIGHT_29=1608,21
PRIMER_LEFT_29_TM=58.484
PRIMER_RIGHT_29_TM=59.565
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=922
PRIMER_PAIR_30_PENALTY=3.992671
PRIMER_LEFT_30_PENALTY=2.661591
PRIMER_RIGHT_30_PENALTY=1.331080
PRIMER_LEFT_30_SEQUENCE=GATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_30_SEQUENCE=CAATGGTGAATTTTGGGATG
PRIMER_LEFT_30=689,20
PRIMER_RIGHT_30=1490,20
PRIMER_LEFT_30_TM=57.338
PRIMER_RIGHT_30_TM=58.669
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.9000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=802
PRIMER_PAIR_31_PENALTY=3.992671
PRIMER_LEFT_31_PENALTY=2.661591
PRIMER_RIGHT_31_PENALTY=1.331080
PRIMER_LEFT_31_SEQUENCE=GGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_31_SEQUENCE=CAATGGTGAATTTTGGGATG
PRIMER_LEFT_31=688,20
PRIMER_RIGHT_31=1490,20
PRIMER_LEFT_31_TM=57.338
PRIMER_RIGHT_31_TM=58.669
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=803
PRIMER_PAIR_32_PENALTY=4.096298
PRIMER_LEFT_32_PENALTY=2.661591
PRIMER_RIGHT_32_PENALTY=1.434707
PRIMER_LEFT_32_SEQUENCE=GATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_32_SEQUENCE=GAAATCAATGCGTCTTGGAAC
PRIMER_LEFT_32=689,20
PRIMER_RIGHT_32=1608,21
PRIMER_LEFT_32_TM=57.338
PRIMER_RIGHT_32_TM=59.565
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.9000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=920
PRIMER_PAIR_33_PENALTY=4.096298
PRIMER_LEFT_33_PENALTY=2.661591
PRIMER_RIGHT_33_PENALTY=1.434707
PRIMER_LEFT_33_SEQUENCE=GGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_33_SEQUENCE=GAAATCAATGCGTCTTGGAAC
PRIMER_LEFT_33=688,20
PRIMER_RIGHT_33=1608,21
PRIMER_LEFT_33_TM=57.338
PRIMER_RIGHT_33_TM=59.565
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=921
PRIMER_PAIR_34_PENALTY=4.117122
PRIMER_LEFT_34_PENALTY=4.106577
PRIMER_RIGHT_34_PENALTY=0.010545
PRIMER_LEFT_34_SEQUENCE=AGGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_34_SEQUENCE=CCTCCTCGCAAAGAAGACAC
PRIMER_LEFT_34=687,22
PRIMER_RIGHT_34=1456,20
PRIMER_LEFT_34_TM=62.107
PRIMER_RIGHT_34_TM=59.989
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.9000
PRIMER_RIGHT_34_END_STABILITY=6.1000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=770
PRIMER_PAIR_35_PENALTY=4.127772
PRIMER_LEFT_35_PENALTY=3.527853
PRIMER_RIGHT_35_PENALTY=0.599919
PRIMER_LEFT_35_SEQUENCE=TCATCATGGAAATAGTGGAGG
PRIMER_RIGHT_35_SEQUENCE=TGGCGGTTGATGTTTATGAC
PRIMER_LEFT_35=830,21
PRIMER_RIGHT_35=1724,20
PRIMER_LEFT_35_TM=57.472
PRIMER_RIGHT_35_TM=59.400
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=6.3000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=895
PRIMER_PAIR_36_PENALTY=4.135617
PRIMER_LEFT_36_PENALTY=4.125071
PRIMER_RIGHT_36_PENALTY=0.010545
PRIMER_LEFT_36_SEQUENCE=ATCATCATGGAAATAGTGGAGG
PRIMER_RIGHT_36_SEQUENCE=CCTCCTCGCAAAGAAGACAC
PRIMER_LEFT_36=829,22
PRIMER_RIGHT_36=1456,20
PRIMER_LEFT_36_TM=57.875
PRIMER_RIGHT_36_TM=59.989
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=6.1000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=628
PRIMER_PAIR_37_PENALTY=4.142151
PRIMER_LEFT_37_PENALTY=3.422919
PRIMER_RIGHT_37_PENALTY=0.719232
PRIMER_LEFT_37_SEQUENCE=AGAGGATTTCATGTTGTGTCGT
PRIMER_RIGHT_37_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_37=685,22
PRIMER_RIGHT_37=1614,20
PRIMER_LEFT_37_TM=58.577
PRIMER_RIGHT_37_TM=59.281
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.8000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=7.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=930
PRIMER_PAIR_38_PENALTY=4.148548
PRIMER_LEFT_38_PENALTY=2.212073
PRIMER_RIGHT_38_PENALTY=1.936476
PRIMER_LEFT_38_SEQUENCE=GGATTTCATGTTGTGTCGTGG
PRIMER_RIGHT_38_SEQUENCE=CCTCCTCGCAAAGAAGACACT
PRIMER_LEFT_38=688,21
PRIMER_RIGHT_38=1456,21
PRIMER_LEFT_38_TM=61.212
PRIMER_RIGHT_38_TM=60.936
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.9000
PRIMER_RIGHT_38_END_STABILITY=6.1000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=769
PRIMER_PAIR_39_PENALTY=4.201185
PRIMER_LEFT_39_PENALTY=3.422919
PRIMER_RIGHT_39_PENALTY=0.778266
PRIMER_LEFT_39_SEQUENCE=AGAGGATTTCATGTTGTGTCGT
PRIMER_RIGHT_39_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_39=685,22
PRIMER_RIGHT_39=1487,20
PRIMER_LEFT_39_TM=58.577
PRIMER_RIGHT_39_TM=59.222
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.8000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=803
PRIMER_PAIR_40_PENALTY=4.201185
PRIMER_LEFT_40_PENALTY=3.422919
PRIMER_RIGHT_40_PENALTY=0.778266
PRIMER_LEFT_40_SEQUENCE=AGAGGATTTCATGTTGTGTCGT
PRIMER_RIGHT_40_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_40=685,22
PRIMER_RIGHT_40=1492,20
PRIMER_LEFT_40_TM=58.577
PRIMER_RIGHT_40_TM=59.222
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.8000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=808
PRIMER_PAIR_41_PENALTY=4.214374
PRIMER_LEFT_41_PENALTY=3.614455
PRIMER_RIGHT_41_PENALTY=0.599919
PRIMER_LEFT_41_SEQUENCE=TCATCATGGAAATAGTGGAGGT
PRIMER_RIGHT_41_SEQUENCE=TGGCGGTTGATGTTTATGAC
PRIMER_LEFT_41=830,22
PRIMER_RIGHT_41=1724,20
PRIMER_LEFT_41_TM=58.386
PRIMER_RIGHT_41_TM=59.400
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=6.3000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=895
PRIMER_PAIR_42_PENALTY=4.223969
PRIMER_LEFT_42_PENALTY=3.504738
PRIMER_RIGHT_42_PENALTY=0.719232
PRIMER_LEFT_42_SEQUENCE=GAGGATTTCATGTTGTGTCGT
PRIMER_RIGHT_42_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_42=686,21
PRIMER_RIGHT_42=1614,20
PRIMER_LEFT_42_TM=57.495
PRIMER_RIGHT_42_TM=59.281
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.8000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=929
PRIMER_PAIR_43_PENALTY=4.247085
PRIMER_LEFT_43_PENALTY=3.527853
PRIMER_RIGHT_43_PENALTY=0.719232
PRIMER_LEFT_43_SEQUENCE=TCATCATGGAAATAGTGGAGG
PRIMER_RIGHT_43_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_43=830,21
PRIMER_RIGHT_43=1614,20
PRIMER_LEFT_43_TM=57.472
PRIMER_RIGHT_43_TM=59.281
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=785
PRIMER_PAIR_44_PENALTY=4.273382
PRIMER_LEFT_44_PENALTY=2.418685
PRIMER_RIGHT_44_PENALTY=1.854696
PRIMER_LEFT_44_SEQUENCE=GAGGATTTCATGTTGTGTCGTG
PRIMER_RIGHT_44_SEQUENCE=CATCCCACGAAGAATTCAAG
PRIMER_LEFT_44=686,22
PRIMER_RIGHT_44=1232,20
PRIMER_LEFT_44_TM=60.419
PRIMER_RIGHT_44_TM=58.145
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.4000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=547
PRIMER_PAIR_45_PENALTY=4.283004
PRIMER_LEFT_45_PENALTY=3.504738
PRIMER_RIGHT_45_PENALTY=0.778266
PRIMER_LEFT_45_SEQUENCE=GAGGATTTCATGTTGTGTCGT
PRIMER_RIGHT_45_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_45=686,21
PRIMER_RIGHT_45=1487,20
PRIMER_LEFT_45_TM=57.495
PRIMER_RIGHT_45_TM=59.222
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.8000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=802
PRIMER_PAIR_46_PENALTY=4.283004
PRIMER_LEFT_46_PENALTY=3.504738
PRIMER_RIGHT_46_PENALTY=0.778266
PRIMER_LEFT_46_SEQUENCE=GAGGATTTCATGTTGTGTCGT
PRIMER_RIGHT_46_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_46=686,21
PRIMER_RIGHT_46=1492,20
PRIMER_LEFT_46_TM=57.495
PRIMER_RIGHT_46_TM=59.222
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.8000
PRIMER_RIGHT_46_END_STABILITY=9.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=807
PRIMER_PAIR_47_PENALTY=4.306120
PRIMER_LEFT_47_PENALTY=3.527853
PRIMER_RIGHT_47_PENALTY=0.778266
PRIMER_LEFT_47_SEQUENCE=TCATCATGGAAATAGTGGAGG
PRIMER_RIGHT_47_SEQUENCE=TGGTGAATTTTGGGATGTTG
PRIMER_LEFT_47=830,21
PRIMER_RIGHT_47=1487,20
PRIMER_LEFT_47_TM=57.472
PRIMER_RIGHT_47_TM=59.222
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=658
PRIMER_PAIR_48_PENALTY=4.306120
PRIMER_LEFT_48_PENALTY=3.527853
PRIMER_RIGHT_48_PENALTY=0.778266
PRIMER_LEFT_48_SEQUENCE=TCATCATGGAAATAGTGGAGG
PRIMER_RIGHT_48_SEQUENCE=CACAATGGTGAATTTTGGGA
PRIMER_LEFT_48=830,21
PRIMER_RIGHT_48=1492,20
PRIMER_LEFT_48_TM=57.472
PRIMER_RIGHT_48_TM=59.222
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=663
PRIMER_PAIR_49_PENALTY=4.333686
PRIMER_LEFT_49_PENALTY=3.614455
PRIMER_RIGHT_49_PENALTY=0.719232
PRIMER_LEFT_49_SEQUENCE=TCATCATGGAAATAGTGGAGGT
PRIMER_RIGHT_49_SEQUENCE=GCAAGTGAAATCAATGCGTC
PRIMER_LEFT_49=830,22
PRIMER_RIGHT_49=1614,20
PRIMER_LEFT_49_TM=58.386
PRIMER_RIGHT_49_TM=59.281
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=785
=
SEQUENCE_ID=MAGIC.149_4_4603881_4604061_edi-0_wil-2_29514_32330_TRUE
SEQUENCE_TARGET=950,280
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNCANATCTCTGTTCAAACATAAAGCTGTNTTAANACANGAACAANAATCNAATCTATTGAAATATNAGGAATCNTTAACANATTCANATTGNNGGTTACNNCTTTGATNAATACNAATTTGCCGNTTTAGAGATGATTNACGAANNNNNNNNNNNNNGATNCCCCNTANGAATCTGGNTTCTNCTANNACTTTGACGCAGATCTATATNTTAANAANNNAATCAAANTCTAGAANTNTANTNNACCAATTTNAAAGNACNAAAANCATAAATCCGANGNNNGAANNTAACTTACNTCTTTGTAGATCTNACAATTTGCTAACNACAAGTNTAGGATGANNANGAAATCATAAGNNNNNNNNNNNNTTTTGTGATTGAANCTTAGATCGACNGANNANNAANNNTTTTTGGNTTCGAATNATTNTTNTTTTTANAGACAACNTTNGTTNACNATNGAATANNNGTATATTAATATACAAAANTNNANAANCAATTCNNNATATAGTTNNGGCCNATNNTNACANNAAANTNGGCCNATATTACAATAAAANATNTTTNNNNNNATCTTATNNNNNNNNNNTTATATNTACNTTNTATNANCTTATGAGNTGTNAAATATGTCTTNGTGTATAAAATTTTAGATGGTTNATGTTGTTTTGTAAAAGTTTAGGTGTTACAAAANGAAAAATGACAATCATCATTGGTCAATANTGNTTAACAATCGGAAGACCATAATTCNATGTTTTTTNCCTTNNACTTTTGGGGTTTTCTACAANTTNCTATTAGTTATANNNNTAGTTTNAAAANTTTANAGCATNNAAGAATTTGANTNGAAAANGTTCAATATGTGTGGAAGTTAAACCATGTATATGTGTGAAAGTATCAATAATTGAGTGAAGATGGTGGCAACNCTGATCTTAAAACGCACAAATCTTATGAGNAAAANCAACAATGCTGNTATTAGAGGTTGNAGGAGATTTGTTTGGAACAAATTATTTGCTACTCTAAAATAAATTATTTTGAATGGTTATTCAGTANTTTTGACGAATGCAACATCAATTTCTTGTGTCCAAAAAGNNNNNNNNNNNNNNANCNTAANGAAGTGGTGGTGGCGGCGNGANTTGNGTGANCTTACATGNNNNNNNNNNNNAGAAAGCTCTCATGGGAAAAATGTCTAGACCAATAAGTTAAANTTGTTTACGTTAAACN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_24016620_24016871_oy-0_bur-0_151385_20720_TRUE
SEQUENCE_TARGET=950,351
SEQUENCE_TEMPLATE=AATCCTCTCTTTCATGTTATTAGAACAGGTTTGAAAGCTGAAGCACTCTCAGTAGTTTCCATAAGAACGCTTCNGCTTCTCAGCTTCGACAACTCACGTCAGTAGATCACCTGAGATTTCANAACAGAACATGANCAAACATAGAGAATGGTAAGTGTNNTGGAATCCTGTGAAATCCTAAATCTATCAAGGATTCAAATCTTGTTATTACNAGAGTCTGATCAAACACACTTAGTTAATCTGAATGATGTTGGAAACTCATGTATGGCTCACAATGCATCAAGCATTACNNNATATATACAGAACCAAACTGGCTCAAGAAACAGATGCAAGACAAATCAAACATNACACANTGAGATTTTGTACAGGCTATTGGAGAAAACCAGATATCAAAATCATGAATTANAANAAAATTCTAAGCCATCTGAATGAAGTAGNTGACTTAATAAGATTCAATTAACAGATGCAGAGAAATCANATACANNTNTNAATTCANCTTCCAAATCGNAANNTAACANTGATATGATCGAAAGAGAAAGACATTACCGCCTGTGTTGTTGGACAAACTTGGAGTTCTTGGCGTCNTCCTCTGCAAAATGAGTCAATACNTTTACTAATTAGGGTTTCACAACCAGCGATCCAACAATCAANACNGAGACACAATACATCAANATATCCGATCCGATTCNACAAATACGGATCAATNGAGGANTAGCTCTTTCATTTCCGATGANGATNGGTCAGATCTGAAGAGACTTACCAGTGAGTCCAGCGGTGAGCTTGGTGATGAGAACGCCGGTAACGGCGAATCCNGTGAGAAACGGCCAACANCGTTTCCATTCTCTCTTGAAGAACACTGGCCATGGATCGAACAACCTCATCNNTCTCTTNTTCTCTTTTTGTTCTCTTTGGTCTTCTCTCNAAACTTTGGTATCTGGATGAGTCGGAGACTGCTTCGCGTAGAGACAGATNAAAGNGNCAACAAAANACTGATGGATAGNTAATAGTTACTGGATCCNAATTCNTTCTGGATANNTAATGGGCTATNNNAAACNCAANNAAGGCCCAATANGTCTTCNAGTTAATTTGTAAAAGGCCANTANTGACNGTTGNNNTTAAAACGCGAATTTCCCGCGACTGCNTTTCAAGCACTCTGGAATTNTTTTNTCTCNTNTNAANNNNNTTNNANTTGNNNNNNNNNNNNCGGTGNTGCTCTNTGANNTAGCTTCAAGATTGATCTGTCTANNNNTTNTTGGTTCGAAGCTATTATTGTGANAGGAAATTGTNAAAGCTTGTTTCATTAGAGAAANCATGGAAAAATGGACCAATTCAANTNANNGACCACTACAAATTCAANTCCGTGTGTATNNCTCTNTCNCTNTGAACATTCTTANTGTATTAGCTATAGANTGATAAACTATATAACAGNTGAGATCAATTNAANTGGTCTTTCAAAAACCAAGTTGCCTNTATNGTCTGACCAAGAAATTTACATATGTTTCTTNGGATAATGTNNNGNTATTCAAGTTNTTGATTGTGATGTCCGGAGATTATTTTCCTAGTCTTCTTGTNTTTTTCNATAGAAAATANNTTTNCTCATTTGTTTCAGTCATNTNCCATTNTTATATTTNCACANTANAGACCTCTTCTCATGTANCCAGAGCTTTTATTTGCCTTNCATGTCCGTTTATGTTTATTTCATACATGCGCTGTTGTTNGTNTCNCNAGGTACCGGAATGATGTCTATGATCGNGNATGGACTACGTTTTCAAGGAATGAATGGANCCACATTTCCACCACCCTGGAAGTGAGCAATTCAAANAANTATTTTCCACCAAAAGAAGCACTCAAAACTGCTGCAATANCTNCTAATTCAANTGCACCATTGACGATGGAATGGAGTNCGANNAATNTCNANNNCCAATATTACTTGTATGGACNTTTTNCTGAGATCCANNANNTGNNNACCAACGATACAAGGGNNNNNNNCATGTTTTGGAATNGACAAGTCATCGCCGNCCCTTTAATACCTCCAAAATTCACNATNTNTACTATCTTTAGCCAGTCACCNNNNNNNNNNNAAGNAGGAAAATGCANTTTTCAGCTGANAAGAACCAACAGATCAACTCTTCCACCTTTGCTTAACGCTTTTGANGTCTACACAGTTATCCAGTTTCCNCAGNNNGAAACAAATGAAAACGACGGTATNTTATGCATATAAGGTCNANACTATTGTTTTATNTTTAGAAAG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.107113
PRIMER_LEFT_0_PENALTY=1.059530
PRIMER_RIGHT_0_PENALTY=0.047584
PRIMER_LEFT_0_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_0_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_0=926,21
PRIMER_RIGHT_0=1549,20
PRIMER_LEFT_0_TM=59.940
PRIMER_RIGHT_0_TM=60.048
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=624
PRIMER_PAIR_1_PENALTY=1.170607
PRIMER_LEFT_1_PENALTY=1.059530
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_1_SEQUENCE=AATTGCTCACTTCCAGGGTG
PRIMER_LEFT_1=926,21
PRIMER_RIGHT_1=1816,20
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=891
PRIMER_PAIR_2_PENALTY=1.191726
PRIMER_LEFT_2_PENALTY=1.080649
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_LEFT_2_SEQUENCE=TGGTATCTGGATGAGTCGGAG
PRIMER_RIGHT_2_SEQUENCE=AATTGCTCACTTCCAGGGTG
PRIMER_LEFT_2=928,21
PRIMER_RIGHT_2=1816,20
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=889
PRIMER_PAIR_3_PENALTY=1.207829
PRIMER_LEFT_3_PENALTY=1.160245
PRIMER_RIGHT_3_PENALTY=0.047584
PRIMER_LEFT_3_SEQUENCE=AAACTTGGAGTTCTTGGCGT
PRIMER_RIGHT_3_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_3=563,20
PRIMER_RIGHT_3=1549,20
PRIMER_LEFT_3_TM=58.840
PRIMER_RIGHT_3_TM=60.048
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=987
PRIMER_PAIR_4_PENALTY=1.245429
PRIMER_LEFT_4_PENALTY=1.197845
PRIMER_RIGHT_4_PENALTY=0.047584
PRIMER_LEFT_4_SEQUENCE=GGTGAGCTTGGTGATGAGAA
PRIMER_RIGHT_4_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_4=773,20
PRIMER_RIGHT_4=1549,20
PRIMER_LEFT_4_TM=58.802
PRIMER_RIGHT_4_TM=60.048
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=777
PRIMER_PAIR_5_PENALTY=1.329174
PRIMER_LEFT_5_PENALTY=1.281590
PRIMER_RIGHT_5_PENALTY=0.047584
PRIMER_LEFT_5_SEQUENCE=GGTGAGCTTGGTGATGAGAAC
PRIMER_RIGHT_5_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_5=773,21
PRIMER_RIGHT_5=1549,20
PRIMER_LEFT_5_TM=59.718
PRIMER_RIGHT_5_TM=60.048
PRIMER_LEFT_5_GC_PERCENT=52.381
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=777
PRIMER_PAIR_6_PENALTY=1.380385
PRIMER_LEFT_6_PENALTY=1.332802
PRIMER_RIGHT_6_PENALTY=0.047584
PRIMER_LEFT_6_SEQUENCE=CATGGATCGAACAACCTCATC
PRIMER_RIGHT_6_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_6=861,21
PRIMER_RIGHT_6=1549,20
PRIMER_LEFT_6_TM=60.333
PRIMER_RIGHT_6_TM=60.048
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=689
PRIMER_PAIR_7_PENALTY=1.443879
PRIMER_LEFT_7_PENALTY=1.332802
PRIMER_RIGHT_7_PENALTY=0.111077
PRIMER_LEFT_7_SEQUENCE=CATGGATCGAACAACCTCATC
PRIMER_RIGHT_7_SEQUENCE=AATTGCTCACTTCCAGGGTG
PRIMER_LEFT_7=861,21
PRIMER_RIGHT_7=1816,20
PRIMER_LEFT_7_TM=60.333
PRIMER_RIGHT_7_TM=60.111
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=956
PRIMER_PAIR_8_PENALTY=1.496647
PRIMER_LEFT_8_PENALTY=1.449063
PRIMER_RIGHT_8_PENALTY=0.047584
PRIMER_LEFT_8_SEQUENCE=ACCAGCGATCCAACAATCAA
PRIMER_RIGHT_8_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_8=630,20
PRIMER_RIGHT_8=1549,20
PRIMER_LEFT_8_TM=61.449
PRIMER_RIGHT_8_TM=60.048
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=920
PRIMER_PAIR_9_PENALTY=1.496647
PRIMER_LEFT_9_PENALTY=1.449063
PRIMER_RIGHT_9_PENALTY=0.047584
PRIMER_LEFT_9_SEQUENCE=AACCAGCGATCCAACAATCA
PRIMER_RIGHT_9_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_9=629,20
PRIMER_RIGHT_9=1549,20
PRIMER_LEFT_9_TM=61.449
PRIMER_RIGHT_9_TM=60.048
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=921
PRIMER_PAIR_10_PENALTY=1.496647
PRIMER_LEFT_10_PENALTY=1.449063
PRIMER_RIGHT_10_PENALTY=0.047584
PRIMER_LEFT_10_SEQUENCE=CAACCAGCGATCCAACAATC
PRIMER_RIGHT_10_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_10=628,20
PRIMER_RIGHT_10=1549,20
PRIMER_LEFT_10_TM=61.449
PRIMER_RIGHT_10_TM=60.048
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=922
PRIMER_PAIR_11_PENALTY=1.532532
PRIMER_LEFT_11_PENALTY=1.484948
PRIMER_RIGHT_11_PENALTY=0.047584
PRIMER_LEFT_11_SEQUENCE=TTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_11_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_11=927,20
PRIMER_RIGHT_11=1549,20
PRIMER_LEFT_11_TM=58.515
PRIMER_RIGHT_11_TM=60.048
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.6000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=623
PRIMER_PAIR_12_PENALTY=1.596025
PRIMER_LEFT_12_PENALTY=1.484948
PRIMER_RIGHT_12_PENALTY=0.111077
PRIMER_LEFT_12_SEQUENCE=TTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_12_SEQUENCE=AATTGCTCACTTCCAGGGTG
PRIMER_LEFT_12=927,20
PRIMER_RIGHT_12=1816,20
PRIMER_LEFT_12_TM=58.515
PRIMER_RIGHT_12_TM=60.111
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=890
PRIMER_PAIR_13_PENALTY=1.644745
PRIMER_LEFT_13_PENALTY=1.059530
PRIMER_RIGHT_13_PENALTY=0.585216
PRIMER_LEFT_13_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_13_SEQUENCE=TGAGTGCTTCTTTTGGTGGA
PRIMER_LEFT_13=926,21
PRIMER_RIGHT_13=1849,20
PRIMER_LEFT_13_TM=59.940
PRIMER_RIGHT_13_TM=59.415
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=924
PRIMER_PAIR_14_PENALTY=1.646052
PRIMER_LEFT_14_PENALTY=1.598468
PRIMER_RIGHT_14_PENALTY=0.047584
PRIMER_LEFT_14_SEQUENCE=TAGGGTTTCACAACCAGCGA
PRIMER_RIGHT_14_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_14=618,20
PRIMER_RIGHT_14=1549,20
PRIMER_LEFT_14_TM=61.598
PRIMER_RIGHT_14_TM=60.048
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=932
PRIMER_PAIR_15_PENALTY=1.665865
PRIMER_LEFT_15_PENALTY=1.080649
PRIMER_RIGHT_15_PENALTY=0.585216
PRIMER_LEFT_15_SEQUENCE=TGGTATCTGGATGAGTCGGAG
PRIMER_RIGHT_15_SEQUENCE=TGAGTGCTTCTTTTGGTGGA
PRIMER_LEFT_15=928,21
PRIMER_RIGHT_15=1849,20
PRIMER_LEFT_15_TM=60.081
PRIMER_RIGHT_15_TM=59.415
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=922
PRIMER_PAIR_16_PENALTY=1.918017
PRIMER_LEFT_16_PENALTY=1.332802
PRIMER_RIGHT_16_PENALTY=0.585216
PRIMER_LEFT_16_SEQUENCE=CATGGATCGAACAACCTCATC
PRIMER_RIGHT_16_SEQUENCE=TGAGTGCTTCTTTTGGTGGA
PRIMER_LEFT_16=861,21
PRIMER_RIGHT_16=1849,20
PRIMER_LEFT_16_TM=60.333
PRIMER_RIGHT_16_TM=59.415
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=989
PRIMER_PAIR_17_PENALTY=1.918742
PRIMER_LEFT_17_PENALTY=1.059530
PRIMER_RIGHT_17_PENALTY=0.859212
PRIMER_LEFT_17_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_17_SEQUENCE=GAATTGCTCACTTCCAGGGT
PRIMER_LEFT_17=926,21
PRIMER_RIGHT_17=1817,20
PRIMER_LEFT_17_TM=59.940
PRIMER_RIGHT_17_TM=59.141
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=9.1000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=892
PRIMER_PAIR_18_PENALTY=1.923718
PRIMER_LEFT_18_PENALTY=1.876134
PRIMER_RIGHT_18_PENALTY=0.047584
PRIMER_LEFT_18_SEQUENCE=CCATGGATCGAACAACCTCA
PRIMER_RIGHT_18_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_18=860,20
PRIMER_RIGHT_18=1549,20
PRIMER_LEFT_18_TM=61.876
PRIMER_RIGHT_18_TM=60.048
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=690
PRIMER_PAIR_19_PENALTY=1.939861
PRIMER_LEFT_19_PENALTY=1.080649
PRIMER_RIGHT_19_PENALTY=0.859212
PRIMER_LEFT_19_SEQUENCE=TGGTATCTGGATGAGTCGGAG
PRIMER_RIGHT_19_SEQUENCE=GAATTGCTCACTTCCAGGGT
PRIMER_LEFT_19=928,21
PRIMER_RIGHT_19=1817,20
PRIMER_LEFT_19_TM=60.081
PRIMER_RIGHT_19_TM=59.141
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=9.1000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=890
PRIMER_PAIR_20_PENALTY=1.987211
PRIMER_LEFT_20_PENALTY=1.876134
PRIMER_RIGHT_20_PENALTY=0.111077
PRIMER_LEFT_20_SEQUENCE=CCATGGATCGAACAACCTCA
PRIMER_RIGHT_20_SEQUENCE=AATTGCTCACTTCCAGGGTG
PRIMER_LEFT_20=860,20
PRIMER_RIGHT_20=1816,20
PRIMER_LEFT_20_TM=61.876
PRIMER_RIGHT_20_TM=60.111
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=957
PRIMER_PAIR_21_PENALTY=2.006408
PRIMER_LEFT_21_PENALTY=1.895331
PRIMER_RIGHT_21_PENALTY=0.111077
PRIMER_LEFT_21_SEQUENCE=GGTATCTGGATGAGTCGGAGA
PRIMER_RIGHT_21_SEQUENCE=AATTGCTCACTTCCAGGGTG
PRIMER_LEFT_21=929,21
PRIMER_RIGHT_21=1816,20
PRIMER_LEFT_21_TM=59.105
PRIMER_RIGHT_21_TM=60.111
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=888
PRIMER_PAIR_22_PENALTY=2.070164
PRIMER_LEFT_22_PENALTY=1.484948
PRIMER_RIGHT_22_PENALTY=0.585216
PRIMER_LEFT_22_SEQUENCE=TTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_22_SEQUENCE=TGAGTGCTTCTTTTGGTGGA
PRIMER_LEFT_22=927,20
PRIMER_RIGHT_22=1849,20
PRIMER_LEFT_22_TM=58.515
PRIMER_RIGHT_22_TM=59.415
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=923
PRIMER_PAIR_23_PENALTY=2.100690
PRIMER_LEFT_23_PENALTY=2.053107
PRIMER_RIGHT_23_PENALTY=0.047584
PRIMER_LEFT_23_SEQUENCE=TTCACAACCAGCGATCCAAC
PRIMER_RIGHT_23_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_23=624,20
PRIMER_RIGHT_23=1549,20
PRIMER_LEFT_23_TM=62.053
PRIMER_RIGHT_23_TM=60.048
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=926
PRIMER_PAIR_24_PENALTY=2.100690
PRIMER_LEFT_24_PENALTY=2.053107
PRIMER_RIGHT_24_PENALTY=0.047584
PRIMER_LEFT_24_SEQUENCE=GTTTCACAACCAGCGATCCA
PRIMER_RIGHT_24_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_24=622,20
PRIMER_RIGHT_24=1549,20
PRIMER_LEFT_24_TM=62.053
PRIMER_RIGHT_24_TM=60.048
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=928
PRIMER_PAIR_25_PENALTY=2.162429
PRIMER_LEFT_25_PENALTY=1.059530
PRIMER_RIGHT_25_PENALTY=1.102899
PRIMER_LEFT_25_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_25_SEQUENCE=TTGAGTGCTTCTTTTGGTGG
PRIMER_LEFT_25=926,21
PRIMER_RIGHT_25=1850,20
PRIMER_LEFT_25_TM=59.940
PRIMER_RIGHT_25_TM=58.897
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=925
PRIMER_PAIR_26_PENALTY=2.183548
PRIMER_LEFT_26_PENALTY=1.080649
PRIMER_RIGHT_26_PENALTY=1.102899
PRIMER_LEFT_26_SEQUENCE=TGGTATCTGGATGAGTCGGAG
PRIMER_RIGHT_26_SEQUENCE=TTGAGTGCTTCTTTTGGTGG
PRIMER_LEFT_26=928,21
PRIMER_RIGHT_26=1850,20
PRIMER_LEFT_26_TM=60.081
PRIMER_RIGHT_26_TM=58.897
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=9.4000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=923
PRIMER_PAIR_27_PENALTY=2.192014
PRIMER_LEFT_27_PENALTY=1.332802
PRIMER_RIGHT_27_PENALTY=0.859212
PRIMER_LEFT_27_SEQUENCE=CATGGATCGAACAACCTCATC
PRIMER_RIGHT_27_SEQUENCE=GAATTGCTCACTTCCAGGGT
PRIMER_LEFT_27=861,21
PRIMER_RIGHT_27=1817,20
PRIMER_LEFT_27_TM=60.333
PRIMER_RIGHT_27_TM=59.141
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.6000
PRIMER_RIGHT_27_END_STABILITY=9.1000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=957
PRIMER_PAIR_28_PENALTY=2.242212
PRIMER_LEFT_28_PENALTY=1.059530
PRIMER_RIGHT_28_PENALTY=1.182682
PRIMER_LEFT_28_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_28_SEQUENCE=GCAGCAGTTTTGAGTGCTTCT
PRIMER_LEFT_28=926,21
PRIMER_RIGHT_28=1859,21
PRIMER_LEFT_28_TM=59.940
PRIMER_RIGHT_28_TM=59.817
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.6000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=934
PRIMER_PAIR_29_PENALTY=2.263331
PRIMER_LEFT_29_PENALTY=1.080649
PRIMER_RIGHT_29_PENALTY=1.182682
PRIMER_LEFT_29_SEQUENCE=TGGTATCTGGATGAGTCGGAG
PRIMER_RIGHT_29_SEQUENCE=GCAGCAGTTTTGAGTGCTTCT
PRIMER_LEFT_29=928,21
PRIMER_RIGHT_29=1859,21
PRIMER_LEFT_29_TM=60.081
PRIMER_RIGHT_29_TM=59.817
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.9000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=932
PRIMER_PAIR_30_PENALTY=2.328626
PRIMER_LEFT_30_PENALTY=1.059530
PRIMER_RIGHT_30_PENALTY=1.269097
PRIMER_LEFT_30_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_30_SEQUENCE=TTTGAGTGCTTCTTTTGGTGG
PRIMER_LEFT_30=926,21
PRIMER_RIGHT_30=1851,21
PRIMER_LEFT_30_TM=59.940
PRIMER_RIGHT_30_TM=60.269
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=2.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=926
PRIMER_PAIR_31_PENALTY=2.344161
PRIMER_LEFT_31_PENALTY=1.484948
PRIMER_RIGHT_31_PENALTY=0.859212
PRIMER_LEFT_31_SEQUENCE=TTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_31_SEQUENCE=GAATTGCTCACTTCCAGGGT
PRIMER_LEFT_31=927,20
PRIMER_RIGHT_31=1817,20
PRIMER_LEFT_31_TM=58.515
PRIMER_RIGHT_31_TM=59.141
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=9.1000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=891
PRIMER_PAIR_32_PENALTY=2.349746
PRIMER_LEFT_32_PENALTY=1.080649
PRIMER_RIGHT_32_PENALTY=1.269097
PRIMER_LEFT_32_SEQUENCE=TGGTATCTGGATGAGTCGGAG
PRIMER_RIGHT_32_SEQUENCE=TTTGAGTGCTTCTTTTGGTGG
PRIMER_LEFT_32=928,21
PRIMER_RIGHT_32=1851,21
PRIMER_LEFT_32_TM=60.081
PRIMER_RIGHT_32_TM=60.269
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.9000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=2.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=924
PRIMER_PAIR_33_PENALTY=2.365963
PRIMER_LEFT_33_PENALTY=2.318379
PRIMER_RIGHT_33_PENALTY=0.047584
PRIMER_LEFT_33_SEQUENCE=GGACAAACTTGGAGTTCTTGG
PRIMER_RIGHT_33_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_33=559,21
PRIMER_RIGHT_33=1549,20
PRIMER_LEFT_33_TM=58.682
PRIMER_RIGHT_33_TM=60.048
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=991
PRIMER_PAIR_34_PENALTY=2.387193
PRIMER_LEFT_34_PENALTY=2.339609
PRIMER_RIGHT_34_PENALTY=0.047584
PRIMER_LEFT_34_SEQUENCE=TGTTGTTGGACAAACTTGGAG
PRIMER_RIGHT_34_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_34=552,21
PRIMER_RIGHT_34=1549,20
PRIMER_LEFT_34_TM=58.660
PRIMER_RIGHT_34_TM=60.048
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=998
PRIMER_PAIR_35_PENALTY=2.435701
PRIMER_LEFT_35_PENALTY=1.332802
PRIMER_RIGHT_35_PENALTY=1.102899
PRIMER_LEFT_35_SEQUENCE=CATGGATCGAACAACCTCATC
PRIMER_RIGHT_35_SEQUENCE=TTGAGTGCTTCTTTTGGTGG
PRIMER_LEFT_35=861,21
PRIMER_RIGHT_35=1850,20
PRIMER_LEFT_35_TM=60.333
PRIMER_RIGHT_35_TM=58.897
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=990
PRIMER_PAIR_36_PENALTY=2.444660
PRIMER_LEFT_36_PENALTY=2.397076
PRIMER_RIGHT_36_PENALTY=0.047584
PRIMER_LEFT_36_SEQUENCE=CGGTGAGCTTGGTGATGAGA
PRIMER_RIGHT_36_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_36=772,20
PRIMER_RIGHT_36=1549,20
PRIMER_LEFT_36_TM=62.397
PRIMER_RIGHT_36_TM=60.048
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=778
PRIMER_PAIR_37_PENALTY=2.461350
PRIMER_LEFT_37_PENALTY=1.876134
PRIMER_RIGHT_37_PENALTY=0.585216
PRIMER_LEFT_37_SEQUENCE=CCATGGATCGAACAACCTCA
PRIMER_RIGHT_37_SEQUENCE=TGAGTGCTTCTTTTGGTGGA
PRIMER_LEFT_37=860,20
PRIMER_RIGHT_37=1849,20
PRIMER_LEFT_37_TM=61.876
PRIMER_RIGHT_37_TM=59.415
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=990
PRIMER_PAIR_38_PENALTY=2.480547
PRIMER_LEFT_38_PENALTY=1.895331
PRIMER_RIGHT_38_PENALTY=0.585216
PRIMER_LEFT_38_SEQUENCE=GGTATCTGGATGAGTCGGAGA
PRIMER_RIGHT_38_SEQUENCE=TGAGTGCTTCTTTTGGTGGA
PRIMER_LEFT_38=929,21
PRIMER_RIGHT_38=1849,20
PRIMER_LEFT_38_TM=59.105
PRIMER_RIGHT_38_TM=59.415
PRIMER_LEFT_38_GC_PERCENT=52.381
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=921
PRIMER_PAIR_39_PENALTY=2.505367
PRIMER_LEFT_39_PENALTY=2.457783
PRIMER_RIGHT_39_PENALTY=0.047584
PRIMER_LEFT_39_SEQUENCE=TGTTGTTGGACAAACTTGGA
PRIMER_RIGHT_39_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_39=552,20
PRIMER_RIGHT_39=1549,20
PRIMER_LEFT_39_TM=57.542
PRIMER_RIGHT_39_TM=60.048
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=998
PRIMER_PAIR_40_PENALTY=2.509323
PRIMER_LEFT_40_PENALTY=2.461740
PRIMER_RIGHT_40_PENALTY=0.047584
PRIMER_LEFT_40_SEQUENCE=GTGTTGTTGGACAAACTTGGAG
PRIMER_RIGHT_40_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_40=551,22
PRIMER_RIGHT_40=1549,20
PRIMER_LEFT_40_TM=59.538
PRIMER_RIGHT_40_TM=60.048
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=999
PRIMER_PAIR_41_PENALTY=2.515484
PRIMER_LEFT_41_PENALTY=1.332802
PRIMER_RIGHT_41_PENALTY=1.182682
PRIMER_LEFT_41_SEQUENCE=CATGGATCGAACAACCTCATC
PRIMER_RIGHT_41_SEQUENCE=GCAGCAGTTTTGAGTGCTTCT
PRIMER_LEFT_41=861,21
PRIMER_RIGHT_41=1859,21
PRIMER_LEFT_41_TM=60.333
PRIMER_RIGHT_41_TM=59.817
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.6000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=999
PRIMER_PAIR_42_PENALTY=2.537742
PRIMER_LEFT_42_PENALTY=2.490158
PRIMER_RIGHT_42_PENALTY=0.047584
PRIMER_LEFT_42_SEQUENCE=GTGTTGTTGGACAAACTTGGA
PRIMER_RIGHT_42_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_42=551,21
PRIMER_RIGHT_42=1549,20
PRIMER_LEFT_42_TM=58.510
PRIMER_RIGHT_42_TM=60.048
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=999
PRIMER_PAIR_43_PENALTY=2.587847
PRIMER_LEFT_43_PENALTY=1.484948
PRIMER_RIGHT_43_PENALTY=1.102899
PRIMER_LEFT_43_SEQUENCE=TTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_43_SEQUENCE=TTGAGTGCTTCTTTTGGTGG
PRIMER_LEFT_43=927,20
PRIMER_RIGHT_43=1850,20
PRIMER_LEFT_43_TM=58.515
PRIMER_RIGHT_43_TM=58.897
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.6000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=924
PRIMER_PAIR_44_PENALTY=2.596922
PRIMER_LEFT_44_PENALTY=2.549338
PRIMER_RIGHT_44_PENALTY=0.047584
PRIMER_LEFT_44_SEQUENCE=TTTCACAACCAGCGATCCAA
PRIMER_RIGHT_44_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_44=623,20
PRIMER_RIGHT_44=1549,20
PRIMER_LEFT_44_TM=62.549
PRIMER_RIGHT_44_TM=60.048
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=927
PRIMER_PAIR_45_PENALTY=2.601898
PRIMER_LEFT_45_PENALTY=1.332802
PRIMER_RIGHT_45_PENALTY=1.269097
PRIMER_LEFT_45_SEQUENCE=CATGGATCGAACAACCTCATC
PRIMER_RIGHT_45_SEQUENCE=TTTGAGTGCTTCTTTTGGTGG
PRIMER_LEFT_45=861,21
PRIMER_RIGHT_45=1851,21
PRIMER_LEFT_45_TM=60.333
PRIMER_RIGHT_45_TM=60.269
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.6000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=991
PRIMER_PAIR_46_PENALTY=2.611866
PRIMER_LEFT_46_PENALTY=2.564282
PRIMER_RIGHT_46_PENALTY=0.047584
PRIMER_LEFT_46_SEQUENCE=CAAACTTGGAGTTCTTGGCGT
PRIMER_RIGHT_46_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_46=562,21
PRIMER_RIGHT_46=1549,20
PRIMER_LEFT_46_TM=61.564
PRIMER_RIGHT_46_TM=60.048
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=11.1000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=988
PRIMER_PAIR_47_PENALTY=2.667631
PRIMER_LEFT_47_PENALTY=1.484948
PRIMER_RIGHT_47_PENALTY=1.182682
PRIMER_LEFT_47_SEQUENCE=TTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_47_SEQUENCE=GCAGCAGTTTTGAGTGCTTCT
PRIMER_LEFT_47=927,20
PRIMER_RIGHT_47=1859,21
PRIMER_LEFT_47_TM=58.515
PRIMER_RIGHT_47_TM=59.817
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=933
PRIMER_PAIR_48_PENALTY=2.694486
PRIMER_LEFT_48_PENALTY=2.646903
PRIMER_RIGHT_48_PENALTY=0.047584
PRIMER_LEFT_48_SEQUENCE=ATGGATCGAACAACCTCATC
PRIMER_RIGHT_48_SEQUENCE=TCTCCGGACATCACAATCAA
PRIMER_LEFT_48=862,20
PRIMER_RIGHT_48=1549,20
PRIMER_LEFT_48_TM=57.353
PRIMER_RIGHT_48_TM=60.048
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=688
PRIMER_PAIR_49_PENALTY=2.714271
PRIMER_LEFT_49_PENALTY=1.059530
PRIMER_RIGHT_49_PENALTY=1.654741
PRIMER_LEFT_49_SEQUENCE=TTTGGTATCTGGATGAGTCGG
PRIMER_RIGHT_49_SEQUENCE=GAGTGCTTCTTTTGGTGGAAA
PRIMER_LEFT_49=926,21
PRIMER_RIGHT_49=1848,21
PRIMER_LEFT_49_TM=59.940
PRIMER_RIGHT_49_TM=59.345
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.105_1_15752733_15753271_can-0_tsu-0_23221_61649_TRUE
SEQUENCE_TARGET=950,638
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5933230_5933248_mt-0_po-0_1313_68033_TRUE
SEQUENCE_TARGET=950,118
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTAGTTTTAAAGTGGCNTTTTTTTNGTGTGGTTCTAGNGTNTNTTNAGTNATTGGNTNTNCTATACANNANATCNTTANNNNNNNNNNNNNNNNNNNNNNCNATANAATGAAANATATACNNTANCNTTTNAAAGAATGAAAAAATAAATTAANAGACAGTTTCGNAGAAGANTTTTTTNNNNNNNNNNNAANTANATGANANATATGAAGAGATTAGTGNCGTGAAGGCNACTCATGGTTTGCNTCTTCTTCTTTAGTTTGTTGCTTTGTTTCAGCATTNGCCACTTTTTTGTCTATCAAAGGCTTTATCATTGCCTCCAAGTCATCTATATCTTGTGTGACTTTTTCCACCTGCNATTTNTNNGTTTTGACTTAGTCAATCACANTCANANNTNTATATATGTTTGNNNATTATTNGGTTCCAAAANATCCCCCAAATGATAAACCAATAAAAGNNACCCAAANANATANATCATATNTTCAAAATGTAATCNTTTACNTTAGCCTTTGTTAGTGTTNNNNNNNNNNNAGATTTTTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNATNAAGTGTNGTGGTNTACCTTGTGAAGGAAATCTTGGGTTANATGTGCTTCATGGGCAGCAACCTCTGAAATNTGTTCTAGAACCAAAGCCACTTGTTTTAGTTTANTTTTCTCAGGCAGTTTCTCTGCCATTTCTTCAGCCATTTCGTCAGTCGCNGTNGCTACTTTCTCCACCATTTCNNCTACAGCCTCTACCCCTTCCACAACCAACTCCGCCTCTCCTGCANNCANNNNATCATTCNANTTNNNNNNGTNCTNNNNNNANNNNANNNNNNNNNNNNNNNNNANNANNNNNNTANNANTNNNANACANNANNNNANCNTANANTNNNATNNAAGNNNNTTNTAGNNAANNNNNATGNNAGNTNTNANANNTNNATTACCTTCNATNCGTTTNAGTTTTTGNANCCTTTNGTTGTTCCAGAAAGATAACACTAANGATATCGCAGAACCTATAACCCATCTTCCCCTGCCNNTTANTCTCCANANNNNANNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNNNNNNNNNNNNNNNNNNACCANTGGTTTGGAGGAGANGGTTNCATCTTGGAGTCANTNGAAACATTACTNAAGTATCTCCCGGCATTANGNGGATTTGATAACTTGAGACCTTGTGGANANATTTGGTTATGATAGTCTCTTCCAAATCGAATAATCGNNGGACATGGATTCANANNTGTNTTCCTCTCNACTTTGTTNTATATTTGTAAGACNTCNGCAAGGCCCTGNCNATACACCGCAAAACCTCCGCTCNGCATTCTCCGACTTAGATACATNNTCAATCNGTTTTATNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNATTCAGTTTNTGTGTGANATTGGTTAAGCGTNTGNTTAAGATANAAGGCTTTTANGTGTAGTAANGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAAATNNNNNNNNNNNNNNNNAACAGAATTTACTTCAAAANGCNATTGAATTAAATAGGAAANCNTCNTTAAAAAATTNCATATTGNAANNTGGANNTATNNNNNNNNNNNATATTAGAATAAATAATTTTCAGCATNTTCNTCAAAGATTTTTTTAAAATAAAAATTAAATAAATTGACATTTTCTTCCTTTCTGGACATAAACCTAAATTAAGGAACATAATATTGAAAGTNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.343665
PRIMER_LEFT_0_PENALTY=0.012411
PRIMER_RIGHT_0_PENALTY=1.331254
PRIMER_LEFT_0_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_0_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_0=492,20
PRIMER_RIGHT_0=1102,21
PRIMER_LEFT_0_TM=59.988
PRIMER_RIGHT_0_TM=59.669
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=611
PRIMER_PAIR_1_PENALTY=1.663282
PRIMER_LEFT_1_PENALTY=0.332028
PRIMER_RIGHT_1_PENALTY=1.331254
PRIMER_LEFT_1_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_1_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_1=363,20
PRIMER_RIGHT_1=1102,21
PRIMER_LEFT_1_TM=59.668
PRIMER_RIGHT_1_TM=59.669
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=740
PRIMER_PAIR_2_PENALTY=2.094919
PRIMER_LEFT_2_PENALTY=0.012411
PRIMER_RIGHT_2_PENALTY=2.082508
PRIMER_LEFT_2_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_2_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_2=492,20
PRIMER_RIGHT_2=1100,22
PRIMER_LEFT_2_TM=59.988
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=609
PRIMER_PAIR_3_PENALTY=2.414536
PRIMER_LEFT_3_PENALTY=0.332028
PRIMER_RIGHT_3_PENALTY=2.082508
PRIMER_LEFT_3_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_3_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_3=363,20
PRIMER_RIGHT_3=1100,22
PRIMER_LEFT_3_TM=59.668
PRIMER_RIGHT_3_TM=59.917
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=738
PRIMER_PAIR_4_PENALTY=2.439670
PRIMER_LEFT_4_PENALTY=0.012411
PRIMER_RIGHT_4_PENALTY=2.427259
PRIMER_LEFT_4_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_4_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_4=492,20
PRIMER_RIGHT_4=1102,22
PRIMER_LEFT_4_TM=59.988
PRIMER_RIGHT_4_TM=60.427
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=611
PRIMER_PAIR_5_PENALTY=2.759287
PRIMER_LEFT_5_PENALTY=0.332028
PRIMER_RIGHT_5_PENALTY=2.427259
PRIMER_LEFT_5_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_5_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_5=363,20
PRIMER_RIGHT_5=1102,22
PRIMER_LEFT_5_TM=59.668
PRIMER_RIGHT_5_TM=60.427
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=740
PRIMER_PAIR_6_PENALTY=3.038153
PRIMER_LEFT_6_PENALTY=0.012411
PRIMER_RIGHT_6_PENALTY=3.025742
PRIMER_LEFT_6_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_6_SEQUENCE=AGGGGAAGATGGGTTATAGG
PRIMER_LEFT_6=492,20
PRIMER_RIGHT_6=1101,20
PRIMER_LEFT_6_TM=59.988
PRIMER_RIGHT_6_TM=56.974
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.1000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=610
PRIMER_PAIR_7_PENALTY=3.053471
PRIMER_LEFT_7_PENALTY=1.722217
PRIMER_RIGHT_7_PENALTY=1.331254
PRIMER_LEFT_7_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_7_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_7=362,20
PRIMER_RIGHT_7=1102,21
PRIMER_LEFT_7_TM=58.278
PRIMER_RIGHT_7_TM=59.669
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=741
PRIMER_PAIR_8_PENALTY=3.094702
PRIMER_LEFT_8_PENALTY=0.012411
PRIMER_RIGHT_8_PENALTY=3.082291
PRIMER_LEFT_8_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_8_SEQUENCE=GGGAAGATGGGTTATAGGTTCTG
PRIMER_LEFT_8=492,20
PRIMER_RIGHT_8=1099,23
PRIMER_LEFT_8_TM=59.988
PRIMER_RIGHT_8_TM=60.082
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=608
PRIMER_PAIR_9_PENALTY=3.119551
PRIMER_LEFT_9_PENALTY=0.332028
PRIMER_RIGHT_9_PENALTY=2.787523
PRIMER_LEFT_9_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_9_SEQUENCE=GGGGAAGATGGGTTATAGGTT
PRIMER_LEFT_9=363,20
PRIMER_RIGHT_9=1100,21
PRIMER_LEFT_9_TM=59.668
PRIMER_RIGHT_9_TM=58.212
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=738
PRIMER_PAIR_10_PENALTY=3.160417
PRIMER_LEFT_10_PENALTY=0.012411
PRIMER_RIGHT_10_PENALTY=3.148006
PRIMER_LEFT_10_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_10_SEQUENCE=AGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_10=492,20
PRIMER_RIGHT_10=1101,21
PRIMER_LEFT_10_TM=59.988
PRIMER_RIGHT_10_TM=57.852
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=610
PRIMER_PAIR_11_PENALTY=3.162311
PRIMER_LEFT_11_PENALTY=1.831057
PRIMER_RIGHT_11_PENALTY=1.331254
PRIMER_LEFT_11_SEQUENCE=CCTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_11_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_11=649,21
PRIMER_RIGHT_11=1102,21
PRIMER_LEFT_11_TM=60.831
PRIMER_RIGHT_11_TM=59.669
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.0000
PRIMER_RIGHT_11_END_STABILITY=7.1000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=454
PRIMER_PAIR_12_PENALTY=3.190666
PRIMER_LEFT_12_PENALTY=0.012411
PRIMER_RIGHT_12_PENALTY=3.178255
PRIMER_LEFT_12_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_12_SEQUENCE=GGGGAAGATGGGTTATAGGT
PRIMER_LEFT_12=492,20
PRIMER_RIGHT_12=1100,20
PRIMER_LEFT_12_TM=59.988
PRIMER_RIGHT_12_TM=56.822
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=609
PRIMER_PAIR_13_PENALTY=3.197244
PRIMER_LEFT_13_PENALTY=0.332028
PRIMER_RIGHT_13_PENALTY=2.865215
PRIMER_LEFT_13_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_13_SEQUENCE=AGGGGAAGATGGGTTATAGGTT
PRIMER_LEFT_13=363,20
PRIMER_RIGHT_13=1101,22
PRIMER_LEFT_13_TM=59.668
PRIMER_RIGHT_13_TM=59.135
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=739
PRIMER_PAIR_14_PENALTY=3.265944
PRIMER_LEFT_14_PENALTY=1.934690
PRIMER_RIGHT_14_PENALTY=1.331254
PRIMER_LEFT_14_SEQUENCE=GGCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_14_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_14=364,21
PRIMER_RIGHT_14=1102,21
PRIMER_LEFT_14_TM=60.935
PRIMER_RIGHT_14_TM=59.669
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=7.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=739
PRIMER_PAIR_15_PENALTY=3.265944
PRIMER_LEFT_15_PENALTY=1.934690
PRIMER_RIGHT_15_PENALTY=1.331254
PRIMER_LEFT_15_SEQUENCE=AAGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_15_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_15=362,21
PRIMER_RIGHT_15=1102,21
PRIMER_LEFT_15_TM=60.935
PRIMER_RIGHT_15_TM=59.669
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=741
PRIMER_PAIR_16_PENALTY=3.357770
PRIMER_LEFT_16_PENALTY=0.332028
PRIMER_RIGHT_16_PENALTY=3.025742
PRIMER_LEFT_16_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_16_SEQUENCE=AGGGGAAGATGGGTTATAGG
PRIMER_LEFT_16=363,20
PRIMER_RIGHT_16=1101,20
PRIMER_LEFT_16_TM=59.668
PRIMER_RIGHT_16_TM=56.974
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.1000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=739
PRIMER_PAIR_17_PENALTY=3.414319
PRIMER_LEFT_17_PENALTY=0.332028
PRIMER_RIGHT_17_PENALTY=3.082291
PRIMER_LEFT_17_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_17_SEQUENCE=GGGAAGATGGGTTATAGGTTCTG
PRIMER_LEFT_17=363,20
PRIMER_RIGHT_17=1099,23
PRIMER_LEFT_17_TM=59.668
PRIMER_RIGHT_17_TM=60.082
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=737
PRIMER_PAIR_18_PENALTY=3.433531
PRIMER_LEFT_18_PENALTY=2.102276
PRIMER_RIGHT_18_PENALTY=1.331254
PRIMER_LEFT_18_SEQUENCE=GCTTTATCATTGCCTCCAAGTC
PRIMER_RIGHT_18_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_18=365,22
PRIMER_RIGHT_18=1102,21
PRIMER_LEFT_18_TM=60.102
PRIMER_RIGHT_18_TM=59.669
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=738
PRIMER_PAIR_19_PENALTY=3.480034
PRIMER_LEFT_19_PENALTY=0.332028
PRIMER_RIGHT_19_PENALTY=3.148006
PRIMER_LEFT_19_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_19_SEQUENCE=AGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_19=363,20
PRIMER_RIGHT_19=1101,21
PRIMER_LEFT_19_TM=59.668
PRIMER_RIGHT_19_TM=57.852
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=739
PRIMER_PAIR_20_PENALTY=3.510283
PRIMER_LEFT_20_PENALTY=0.332028
PRIMER_RIGHT_20_PENALTY=3.178255
PRIMER_LEFT_20_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_20_SEQUENCE=GGGGAAGATGGGTTATAGGT
PRIMER_LEFT_20=363,20
PRIMER_RIGHT_20=1100,20
PRIMER_LEFT_20_TM=59.668
PRIMER_RIGHT_20_TM=56.822
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=738
PRIMER_PAIR_21_PENALTY=3.758362
PRIMER_LEFT_21_PENALTY=0.012411
PRIMER_RIGHT_21_PENALTY=3.745951
PRIMER_LEFT_21_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_21_SEQUENCE=GGGGAAGATGGGTTATAGGTTCT
PRIMER_LEFT_21=492,20
PRIMER_RIGHT_21=1100,23
PRIMER_LEFT_21_TM=59.988
PRIMER_RIGHT_21_TM=60.746
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=47.826
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=609
PRIMER_PAIR_22_PENALTY=3.758362
PRIMER_LEFT_22_PENALTY=0.012411
PRIMER_RIGHT_22_PENALTY=3.745951
PRIMER_LEFT_22_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_22_SEQUENCE=AGGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_22=492,20
PRIMER_RIGHT_22=1101,23
PRIMER_LEFT_22_TM=59.988
PRIMER_RIGHT_22_TM=60.746
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=47.826
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=610
PRIMER_PAIR_23_PENALTY=3.804724
PRIMER_LEFT_23_PENALTY=1.722217
PRIMER_RIGHT_23_PENALTY=2.082508
PRIMER_LEFT_23_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_23_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_23=362,20
PRIMER_RIGHT_23=1100,22
PRIMER_LEFT_23_TM=58.278
PRIMER_RIGHT_23_TM=59.917
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=739
PRIMER_PAIR_24_PENALTY=3.831805
PRIMER_LEFT_24_PENALTY=2.500551
PRIMER_RIGHT_24_PENALTY=1.331254
PRIMER_LEFT_24_SEQUENCE=CTTGTGAAGGAAATCTTGGGTT
PRIMER_RIGHT_24_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_24=650,22
PRIMER_RIGHT_24=1102,21
PRIMER_LEFT_24_TM=59.499
PRIMER_RIGHT_24_TM=59.669
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=7.1000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=453
PRIMER_PAIR_25_PENALTY=3.852531
PRIMER_LEFT_25_PENALTY=1.770024
PRIMER_RIGHT_25_PENALTY=2.082508
PRIMER_LEFT_25_SEQUENCE=TCATTGCCTCCAAGTCATCT
PRIMER_RIGHT_25_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_25=371,20
PRIMER_RIGHT_25=1100,22
PRIMER_LEFT_25_TM=58.230
PRIMER_RIGHT_25_TM=59.917
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=730
PRIMER_PAIR_26_PENALTY=3.875336
PRIMER_LEFT_26_PENALTY=2.544082
PRIMER_RIGHT_26_PENALTY=1.331254
PRIMER_LEFT_26_SEQUENCE=GCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_26_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_26=365,20
PRIMER_RIGHT_26=1102,21
PRIMER_LEFT_26_TM=57.456
PRIMER_RIGHT_26_TM=59.669
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=7.1000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=738
PRIMER_PAIR_27_PENALTY=3.913565
PRIMER_LEFT_27_PENALTY=1.831057
PRIMER_RIGHT_27_PENALTY=2.082508
PRIMER_LEFT_27_SEQUENCE=CCTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_27_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_27=649,21
PRIMER_RIGHT_27=1100,22
PRIMER_LEFT_27_TM=60.831
PRIMER_RIGHT_27_TM=59.917
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=10.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=452
PRIMER_PAIR_28_PENALTY=3.990140
PRIMER_LEFT_28_PENALTY=2.658886
PRIMER_RIGHT_28_PENALTY=1.331254
PRIMER_LEFT_28_SEQUENCE=GCTTTATCATTGCCTCCAAGT
PRIMER_RIGHT_28_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_28=365,21
PRIMER_RIGHT_28=1102,21
PRIMER_LEFT_28_TM=58.341
PRIMER_RIGHT_28_TM=59.669
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=738
PRIMER_PAIR_29_PENALTY=3.993317
PRIMER_LEFT_29_PENALTY=2.662063
PRIMER_RIGHT_29_PENALTY=1.331254
PRIMER_LEFT_29_SEQUENCE=CTTCATGGGCAGCAACCTCT
PRIMER_RIGHT_29_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_29=679,20
PRIMER_RIGHT_29=1102,21
PRIMER_LEFT_29_TM=62.662
PRIMER_RIGHT_29_TM=59.669
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=7.1000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=424
PRIMER_PAIR_30_PENALTY=4.017198
PRIMER_LEFT_30_PENALTY=1.934690
PRIMER_RIGHT_30_PENALTY=2.082508
PRIMER_LEFT_30_SEQUENCE=GGCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_30_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_30=364,21
PRIMER_RIGHT_30=1100,22
PRIMER_LEFT_30_TM=60.935
PRIMER_RIGHT_30_TM=59.917
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=737
PRIMER_PAIR_31_PENALTY=4.017198
PRIMER_LEFT_31_PENALTY=1.934690
PRIMER_RIGHT_31_PENALTY=2.082508
PRIMER_LEFT_31_SEQUENCE=AAGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_31_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_31=362,21
PRIMER_RIGHT_31=1100,22
PRIMER_LEFT_31_TM=60.935
PRIMER_RIGHT_31_TM=59.917
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=739
PRIMER_PAIR_32_PENALTY=4.077979
PRIMER_LEFT_32_PENALTY=0.332028
PRIMER_RIGHT_32_PENALTY=3.745951
PRIMER_LEFT_32_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_32_SEQUENCE=GGGGAAGATGGGTTATAGGTTCT
PRIMER_LEFT_32=363,20
PRIMER_RIGHT_32=1100,23
PRIMER_LEFT_32_TM=59.668
PRIMER_RIGHT_32_TM=60.746
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.826
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=738
PRIMER_PAIR_33_PENALTY=4.077979
PRIMER_LEFT_33_PENALTY=0.332028
PRIMER_RIGHT_33_PENALTY=3.745951
PRIMER_LEFT_33_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_33_SEQUENCE=AGGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_33=363,20
PRIMER_RIGHT_33=1101,23
PRIMER_LEFT_33_TM=59.668
PRIMER_RIGHT_33_TM=60.746
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=47.826
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=739
PRIMER_PAIR_34_PENALTY=4.089047
PRIMER_LEFT_34_PENALTY=2.757793
PRIMER_RIGHT_34_PENALTY=1.331254
PRIMER_LEFT_34_SEQUENCE=CTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_34_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_34=650,20
PRIMER_RIGHT_34=1102,21
PRIMER_LEFT_34_TM=57.242
PRIMER_RIGHT_34_TM=59.669
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.0000
PRIMER_RIGHT_34_END_STABILITY=7.1000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=453
PRIMER_PAIR_35_PENALTY=4.089047
PRIMER_LEFT_35_PENALTY=2.757793
PRIMER_RIGHT_35_PENALTY=1.331254
PRIMER_LEFT_35_SEQUENCE=CCTTGTGAAGGAAATCTTGG
PRIMER_RIGHT_35_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_35=649,20
PRIMER_RIGHT_35=1102,21
PRIMER_LEFT_35_TM=57.242
PRIMER_RIGHT_35_TM=59.669
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=7.1000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=454
PRIMER_PAIR_36_PENALTY=4.149476
PRIMER_LEFT_36_PENALTY=1.722217
PRIMER_RIGHT_36_PENALTY=2.427259
PRIMER_LEFT_36_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_36_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_36=362,20
PRIMER_RIGHT_36=1102,22
PRIMER_LEFT_36_TM=58.278
PRIMER_RIGHT_36_TM=60.427
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=741
PRIMER_PAIR_37_PENALTY=4.170222
PRIMER_LEFT_37_PENALTY=2.838968
PRIMER_RIGHT_37_PENALTY=1.331254
PRIMER_LEFT_37_SEQUENCE=CTTGTGAAGGAAATCTTGGGT
PRIMER_RIGHT_37_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_37=650,21
PRIMER_RIGHT_37=1102,21
PRIMER_LEFT_37_TM=58.161
PRIMER_RIGHT_37_TM=59.669
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=7.1000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=453
PRIMER_PAIR_38_PENALTY=4.170222
PRIMER_LEFT_38_PENALTY=2.838968
PRIMER_RIGHT_38_PENALTY=1.331254
PRIMER_LEFT_38_SEQUENCE=ACCTTGTGAAGGAAATCTTGG
PRIMER_RIGHT_38_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_38=648,21
PRIMER_RIGHT_38=1102,21
PRIMER_LEFT_38_TM=58.161
PRIMER_RIGHT_38_TM=59.669
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=7.1000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=455
PRIMER_PAIR_39_PENALTY=4.184784
PRIMER_LEFT_39_PENALTY=2.102276
PRIMER_RIGHT_39_PENALTY=2.082508
PRIMER_LEFT_39_SEQUENCE=GCTTTATCATTGCCTCCAAGTC
PRIMER_RIGHT_39_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_39=365,22
PRIMER_RIGHT_39=1100,22
PRIMER_LEFT_39_TM=60.102
PRIMER_RIGHT_39_TM=59.917
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=736
PRIMER_PAIR_40_PENALTY=4.248738
PRIMER_LEFT_40_PENALTY=2.917484
PRIMER_RIGHT_40_PENALTY=1.331254
PRIMER_LEFT_40_SEQUENCE=TGTGAAGGAAATCTTGGGTT
PRIMER_RIGHT_40_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_40=652,20
PRIMER_RIGHT_40=1102,21
PRIMER_LEFT_40_TM=57.083
PRIMER_RIGHT_40_TM=59.669
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.1000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=451
PRIMER_PAIR_41_PENALTY=4.248738
PRIMER_LEFT_41_PENALTY=2.917484
PRIMER_RIGHT_41_PENALTY=1.331254
PRIMER_LEFT_41_SEQUENCE=TTGTGAAGGAAATCTTGGGT
PRIMER_RIGHT_41_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_41=651,20
PRIMER_RIGHT_41=1102,21
PRIMER_LEFT_41_TM=57.083
PRIMER_RIGHT_41_TM=59.669
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=7.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=452
PRIMER_PAIR_42_PENALTY=4.258316
PRIMER_LEFT_42_PENALTY=1.831057
PRIMER_RIGHT_42_PENALTY=2.427259
PRIMER_LEFT_42_SEQUENCE=CCTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_42_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_42=649,21
PRIMER_RIGHT_42=1102,22
PRIMER_LEFT_42_TM=60.831
PRIMER_RIGHT_42_TM=60.427
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.0000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=454
PRIMER_PAIR_43_PENALTY=4.353137
PRIMER_LEFT_43_PENALTY=3.021883
PRIMER_RIGHT_43_PENALTY=1.331254
PRIMER_LEFT_43_SEQUENCE=ATGGGCAGCAACCTCTGAAA
PRIMER_RIGHT_43_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_43=683,20
PRIMER_RIGHT_43=1102,21
PRIMER_LEFT_43_TM=63.022
PRIMER_RIGHT_43_TM=59.669
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=7.1000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=420
PRIMER_PAIR_44_PENALTY=4.361949
PRIMER_LEFT_44_PENALTY=1.934690
PRIMER_RIGHT_44_PENALTY=2.427259
PRIMER_LEFT_44_SEQUENCE=GGCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_44_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_44=364,21
PRIMER_RIGHT_44=1102,22
PRIMER_LEFT_44_TM=60.935
PRIMER_RIGHT_44_TM=60.427
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=739
PRIMER_PAIR_45_PENALTY=4.361949
PRIMER_LEFT_45_PENALTY=1.934690
PRIMER_RIGHT_45_PENALTY=2.427259
PRIMER_LEFT_45_SEQUENCE=AAGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_45_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_45=362,21
PRIMER_RIGHT_45=1102,22
PRIMER_LEFT_45_TM=60.935
PRIMER_RIGHT_45_TM=60.427
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=741
PRIMER_PAIR_46_PENALTY=4.456579
PRIMER_LEFT_46_PENALTY=0.012411
PRIMER_RIGHT_46_PENALTY=4.444168
PRIMER_LEFT_46_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_46_SEQUENCE=GGGAAGATGGGTTATAGGTTCT
PRIMER_LEFT_46=492,20
PRIMER_RIGHT_46=1099,22
PRIMER_LEFT_46_TM=59.988
PRIMER_RIGHT_46_TM=57.556
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=608
PRIMER_PAIR_47_PENALTY=4.473129
PRIMER_LEFT_47_PENALTY=2.390622
PRIMER_RIGHT_47_PENALTY=2.082508
PRIMER_LEFT_47_SEQUENCE=ATCATTGCCTCCAAGTCATCT
PRIMER_RIGHT_47_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_47=370,21
PRIMER_RIGHT_47=1100,22
PRIMER_LEFT_47_TM=58.609
PRIMER_RIGHT_47_TM=59.917
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=731
PRIMER_PAIR_48_PENALTY=4.475882
PRIMER_LEFT_48_PENALTY=0.012411
PRIMER_RIGHT_48_PENALTY=4.463471
PRIMER_LEFT_48_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_48_SEQUENCE=GGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_48=492,20
PRIMER_RIGHT_48=1099,21
PRIMER_LEFT_48_TM=59.988
PRIMER_RIGHT_48_TM=56.537
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=608
PRIMER_PAIR_49_PENALTY=4.509740
PRIMER_LEFT_49_PENALTY=1.722217
PRIMER_RIGHT_49_PENALTY=2.787523
PRIMER_LEFT_49_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_49_SEQUENCE=GGGGAAGATGGGTTATAGGTT
PRIMER_LEFT_49=362,20
PRIMER_RIGHT_49=1100,21
PRIMER_LEFT_49_TM=58.278
PRIMER_RIGHT_49_TM=58.212
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=739
=
SEQUENCE_ID=MAGIC.149_3_12554974_12555106_po-0_oy-0_49871_207111_TRUE
SEQUENCE_TARGET=950,232
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_23019491_23019788_can-0_wu-0_561_439338_TRUE
SEQUENCE_TARGET=950,397
SEQUENCE_TEMPLATE=GTTTATTAATGGGGCTGGTAGAGATTGTGGTGCATTGTGGAGACATTCTTATAGGATTTGTAGTTTCTAAAATTAGGGTTTGCATGGAGCTAGGGAGAGATTTAAGNTCGTTGAATGGCTAAAAATTCTCTAATNNAATATTGGCTATGAGACTAAAAATNGGTCACCACAAGATGATTTGATTATGTCCTGATCAAGAGTTCGATTACTAAAAATATNAAACGTAGATCCAANCAAAAAAANTGTATGTGGCAGTAACTCTAGTTTACTNNTACATATAACATATCCTCTNATTCTAATCTTGTGAATNTATTTTGGANTATTCATTCCTAAGTTTAGTNATTTATCCGAAAGTAAAGTATTCGAAAACTTCTTATTGAGAGTTTNCTCTCATCCTTTTAAGGTTAAAACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTATAACGAAAGGATTATGAGCTTNAAGATTCAAAATTAGTTTTGNTTAAAGTGTTTGAATAGAAAAACCGAANTNGTAAGCNTTTATTCAATAGAAANNNNNAAAAAANCGAAATCATATGATACNNNNNNNNTNANNCANATNTNNNTNNACNTNNNNCNANNNNNTGNNCNANNNNATNNGNTANNNTNTNCGNTNAACNNTCNNNNNNCTNNNATNNTANNATTGATGACGCAAAACGGTAAAAAATACNAAAATAGCAATAACGATTANGGAATGATGATAATAAAAAAGAGAGAGANAATTTGCATCATAGATATCACGANATTAGAGAGATTTAATTNNNNNNNNNNNNNTNNTNNTNNNNNNNGGTTAACNGTTAANNNNNNGTNAATTGTCAANNNNCAANAACNGNANNTNNNTNANNNANNCNCNNTNATNTATATANANANNAAANNAACTNNNNACNCGNATNNACNNTNGANTNTGNNNANNTTNAAATGAGATTAGCAGATAATATAANTTTTTTGCAGATTTCAAAATTTCCTGAGGCTAAATGNCTAAATCATCATTCTAATACTTTNGTTATCATTAACNANATGTATTTAAAANAAAAATGTTGAAANAGNGACAAAAAATAATAACATTTCATTAATTAATATATCCTTTCCAATCATCACGAAATCCGTCCGTNCATNTATAAAACCTACAACATTACATCACAAACGACGCATAATCATACATGACAGAACCAACCATCTATGATTCCATTCGCGATTGCTCTAGGCCTTNNACGATATAATTAATTATTACATCCAATCCCTTAATACGATCATCAGTAATAATAATCAGACAAGGGTGGCGGTCGGGGCGAAGTGTCTTCCTCGTTAAGCTTCACCTTAATACCAAAAGTCTTGAAAATAGTATTCAAGTTATTAAACACATCAAAAGAATCACTTGTTAGATTCTTCCCAACTCCAATCCCAATCCCCACTTCCTTCAACAAAATCTTGTCTTCAATATCGTCCAAACAAGTCGAATGATACCCCATGATTGACATTAACTCTTTTTTACACGTAAAATAATTATGAGCCAACGTCGTTACATCTTTCCCCGTAGCTTTCCAAAAATCGGCGAAATCTTCCAACGCGTAATTCAAGTTTTTCTCGCAGCTGTTGACCACTGCGGTTGCGGTTGCGTCTGACATGTGTTTTGGTTTGATTCCAGAGAGGAAAGTCACACTTTGTTTGACGGATGTTTCAGCGGCAGNCACTAATGCTCGGATTAGTTCGATGGGGTCATTGGTAGGAACGTGTTTAAGGGTTTTGCAACATAGACTTGTGTCCGTTGGGATAACGGTGCAGATTCCCGCGACTGCTTTGGCGTGCGGTGTGAAAAGGTAGTTCGTTCGTTTGNCTGGTTTAGCCGTTGCGGTTGCGGCAACGAATATGAAGAGAAAGAAGAGTTTGATTGGAGTGTTCATGATTGTGTTTTCGTATGAACGGAGAAAGGGATCGGAAAGGNACGGATCTCGNAGGAAGAAGAGAGGTTTTTTGATANNNNTAGAAAGATAATGCATGTTTTGTNTATATTTATAGTTCGTCTATATTTGGNAGCTAGCTTNNNNNNTTTTTTTTTGTTAANAATTANATANGGTAGGGAGTGAATTGCGCGTTAATTTTCGGGTTAAACGGTTGTGTANTATNTTAGTTGATGNTGACNNAATNATATTACANNTTTTAGNTTNNNAAGAAAATGATATTTAGAGTTTAGAANAAAGTNCTCAAATCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.169123
PRIMER_LEFT_0_PENALTY=0.110660
PRIMER_RIGHT_0_PENALTY=0.058463
PRIMER_LEFT_0_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_0_SEQUENCE=TTTTGTTGAAGGAAGTGGGG
PRIMER_LEFT_0=732,20
PRIMER_RIGHT_0=1513,20
PRIMER_LEFT_0_TM=60.111
PRIMER_RIGHT_0_TM=59.942
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=782
PRIMER_PAIR_1_PENALTY=0.169123
PRIMER_LEFT_1_PENALTY=0.110660
PRIMER_RIGHT_1_PENALTY=0.058463
PRIMER_LEFT_1_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_1_SEQUENCE=TTTTGTTGAAGGAAGTGGGG
PRIMER_LEFT_1=731,20
PRIMER_RIGHT_1=1513,20
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=59.942
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=783
PRIMER_PAIR_2_PENALTY=0.395823
PRIMER_LEFT_2_PENALTY=0.110660
PRIMER_RIGHT_2_PENALTY=0.285163
PRIMER_LEFT_2_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_2_SEQUENCE=CGATTTTTGGAAAGCTACGG
PRIMER_LEFT_2=732,20
PRIMER_RIGHT_2=1638,20
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=59.715
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=907
PRIMER_PAIR_3_PENALTY=0.395823
PRIMER_LEFT_3_PENALTY=0.110660
PRIMER_RIGHT_3_PENALTY=0.285163
PRIMER_LEFT_3_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_3_SEQUENCE=CGATTTTTGGAAAGCTACGG
PRIMER_LEFT_3=731,20
PRIMER_RIGHT_3=1638,20
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.715
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=908
PRIMER_PAIR_4_PENALTY=0.415304
PRIMER_LEFT_4_PENALTY=0.110660
PRIMER_RIGHT_4_PENALTY=0.304643
PRIMER_LEFT_4_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_4_SEQUENCE=ATTTTTGGAAAGCTACGGGG
PRIMER_LEFT_4=732,20
PRIMER_RIGHT_4=1636,20
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=60.305
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=905
PRIMER_PAIR_5_PENALTY=0.415304
PRIMER_LEFT_5_PENALTY=0.110660
PRIMER_RIGHT_5_PENALTY=0.304643
PRIMER_LEFT_5_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_5_SEQUENCE=ATTTTTGGAAAGCTACGGGG
PRIMER_LEFT_5=731,20
PRIMER_RIGHT_5=1636,20
PRIMER_LEFT_5_TM=60.111
PRIMER_RIGHT_5_TM=60.305
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=12.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=906
PRIMER_PAIR_6_PENALTY=0.419382
PRIMER_LEFT_6_PENALTY=0.110660
PRIMER_RIGHT_6_PENALTY=0.308722
PRIMER_LEFT_6_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_6_SEQUENCE=CATTCGACTTGTTTGGACGA
PRIMER_LEFT_6=732,20
PRIMER_RIGHT_6=1547,20
PRIMER_LEFT_6_TM=60.111
PRIMER_RIGHT_6_TM=59.691
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.0000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=816
PRIMER_PAIR_7_PENALTY=0.419382
PRIMER_LEFT_7_PENALTY=0.110660
PRIMER_RIGHT_7_PENALTY=0.308722
PRIMER_LEFT_7_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_7_SEQUENCE=CATTCGACTTGTTTGGACGA
PRIMER_LEFT_7=731,20
PRIMER_RIGHT_7=1547,20
PRIMER_LEFT_7_TM=60.111
PRIMER_RIGHT_7_TM=59.691
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.2000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=817
PRIMER_PAIR_8_PENALTY=0.450475
PRIMER_LEFT_8_PENALTY=0.110660
PRIMER_RIGHT_8_PENALTY=0.339815
PRIMER_LEFT_8_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_8_SEQUENCE=GAAGATTTCGCCGATTTTTG
PRIMER_LEFT_8=731,20
PRIMER_RIGHT_8=1649,20
PRIMER_LEFT_8_TM=60.111
PRIMER_RIGHT_8_TM=59.660
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.2000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=919
PRIMER_PAIR_9_PENALTY=0.507951
PRIMER_LEFT_9_PENALTY=0.110660
PRIMER_RIGHT_9_PENALTY=0.397290
PRIMER_LEFT_9_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_9_SEQUENCE=AACACATGTCAGACGCAACC
PRIMER_LEFT_9=732,20
PRIMER_RIGHT_9=1717,20
PRIMER_LEFT_9_TM=60.111
PRIMER_RIGHT_9_TM=59.603
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.0000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=986
PRIMER_PAIR_10_PENALTY=0.507951
PRIMER_LEFT_10_PENALTY=0.110660
PRIMER_RIGHT_10_PENALTY=0.397290
PRIMER_LEFT_10_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_10_SEQUENCE=AACACATGTCAGACGCAACC
PRIMER_LEFT_10=731,20
PRIMER_RIGHT_10=1717,20
PRIMER_LEFT_10_TM=60.111
PRIMER_RIGHT_10_TM=59.603
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=987
PRIMER_PAIR_11_PENALTY=0.575174
PRIMER_LEFT_11_PENALTY=0.110660
PRIMER_RIGHT_11_PENALTY=0.464514
PRIMER_LEFT_11_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_11_SEQUENCE=TTTGTTGAAGGAAGTGGGGA
PRIMER_LEFT_11=732,20
PRIMER_RIGHT_11=1512,20
PRIMER_LEFT_11_TM=60.111
PRIMER_RIGHT_11_TM=60.465
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.0000
PRIMER_RIGHT_11_END_STABILITY=10.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=781
PRIMER_PAIR_12_PENALTY=0.575174
PRIMER_LEFT_12_PENALTY=0.110660
PRIMER_RIGHT_12_PENALTY=0.464514
PRIMER_LEFT_12_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_12_SEQUENCE=TTTGTTGAAGGAAGTGGGGA
PRIMER_LEFT_12=731,20
PRIMER_RIGHT_12=1512,20
PRIMER_LEFT_12_TM=60.111
PRIMER_RIGHT_12_TM=60.465
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=10.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=782
PRIMER_PAIR_13_PENALTY=0.729710
PRIMER_LEFT_13_PENALTY=0.110660
PRIMER_RIGHT_13_PENALTY=0.619050
PRIMER_LEFT_13_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_13_SEQUENCE=GTTGAAGGAAGTGGGGATTG
PRIMER_LEFT_13=732,20
PRIMER_RIGHT_13=1509,20
PRIMER_LEFT_13_TM=60.111
PRIMER_RIGHT_13_TM=59.381
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=778
PRIMER_PAIR_14_PENALTY=0.729710
PRIMER_LEFT_14_PENALTY=0.110660
PRIMER_RIGHT_14_PENALTY=0.619050
PRIMER_LEFT_14_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_14_SEQUENCE=TGTTGAAGGAAGTGGGGATT
PRIMER_LEFT_14=732,20
PRIMER_RIGHT_14=1510,20
PRIMER_LEFT_14_TM=60.111
PRIMER_RIGHT_14_TM=59.381
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.0000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=779
PRIMER_PAIR_15_PENALTY=0.729710
PRIMER_LEFT_15_PENALTY=0.110660
PRIMER_RIGHT_15_PENALTY=0.619050
PRIMER_LEFT_15_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_15_SEQUENCE=GTTGAAGGAAGTGGGGATTG
PRIMER_LEFT_15=731,20
PRIMER_RIGHT_15=1509,20
PRIMER_LEFT_15_TM=60.111
PRIMER_RIGHT_15_TM=59.381
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.2000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=779
PRIMER_PAIR_16_PENALTY=0.729710
PRIMER_LEFT_16_PENALTY=0.110660
PRIMER_RIGHT_16_PENALTY=0.619050
PRIMER_LEFT_16_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_16_SEQUENCE=TGTTGAAGGAAGTGGGGATT
PRIMER_LEFT_16=731,20
PRIMER_RIGHT_16=1510,20
PRIMER_LEFT_16_TM=60.111
PRIMER_RIGHT_16_TM=59.381
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=780
PRIMER_PAIR_17_PENALTY=0.768736
PRIMER_LEFT_17_PENALTY=0.110660
PRIMER_RIGHT_17_PENALTY=0.658076
PRIMER_LEFT_17_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_17_SEQUENCE=CAGCTGCGAGAAAAACTTGA
PRIMER_LEFT_17=732,20
PRIMER_RIGHT_17=1680,20
PRIMER_LEFT_17_TM=60.111
PRIMER_RIGHT_17_TM=59.342
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.0000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=949
PRIMER_PAIR_18_PENALTY=0.768736
PRIMER_LEFT_18_PENALTY=0.110660
PRIMER_RIGHT_18_PENALTY=0.658076
PRIMER_LEFT_18_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_18_SEQUENCE=CAGCTGCGAGAAAAACTTGA
PRIMER_LEFT_18=731,20
PRIMER_RIGHT_18=1680,20
PRIMER_LEFT_18_TM=60.111
PRIMER_RIGHT_18_TM=59.342
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=950
PRIMER_PAIR_19_PENALTY=0.809650
PRIMER_LEFT_19_PENALTY=0.110660
PRIMER_RIGHT_19_PENALTY=0.698990
PRIMER_LEFT_19_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_19_SEQUENCE=TTGAATTACGCGTTGGAAGA
PRIMER_LEFT_19=732,20
PRIMER_RIGHT_19=1664,20
PRIMER_LEFT_19_TM=60.111
PRIMER_RIGHT_19_TM=59.301
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.0000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=933
PRIMER_PAIR_20_PENALTY=0.809650
PRIMER_LEFT_20_PENALTY=0.110660
PRIMER_RIGHT_20_PENALTY=0.698990
PRIMER_LEFT_20_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_20_SEQUENCE=TTGAATTACGCGTTGGAAGA
PRIMER_LEFT_20=731,20
PRIMER_RIGHT_20=1664,20
PRIMER_LEFT_20_TM=60.111
PRIMER_RIGHT_20_TM=59.301
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=934
PRIMER_PAIR_21_PENALTY=0.813116
PRIMER_LEFT_21_PENALTY=0.110660
PRIMER_RIGHT_21_PENALTY=0.702456
PRIMER_LEFT_21_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_21_SEQUENCE=CAAAACACATGTCAGACGCA
PRIMER_LEFT_21=732,20
PRIMER_RIGHT_21=1720,20
PRIMER_LEFT_21_TM=60.111
PRIMER_RIGHT_21_TM=59.298
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.0000
PRIMER_RIGHT_21_END_STABILITY=9.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=989
PRIMER_PAIR_22_PENALTY=0.813116
PRIMER_LEFT_22_PENALTY=0.110660
PRIMER_RIGHT_22_PENALTY=0.702456
PRIMER_LEFT_22_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_22_SEQUENCE=CAAAACACATGTCAGACGCA
PRIMER_LEFT_22=731,20
PRIMER_RIGHT_22=1720,20
PRIMER_LEFT_22_TM=60.111
PRIMER_RIGHT_22_TM=59.298
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.2000
PRIMER_RIGHT_22_END_STABILITY=9.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=990
PRIMER_PAIR_23_PENALTY=0.911748
PRIMER_LEFT_23_PENALTY=0.110660
PRIMER_RIGHT_23_PENALTY=0.801088
PRIMER_LEFT_23_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_23_SEQUENCE=TCAACAGCTGCGAGAAAAAC
PRIMER_LEFT_23=732,20
PRIMER_RIGHT_23=1684,20
PRIMER_LEFT_23_TM=60.111
PRIMER_RIGHT_23_TM=59.199
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.0000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=953
PRIMER_PAIR_24_PENALTY=0.911748
PRIMER_LEFT_24_PENALTY=0.110660
PRIMER_RIGHT_24_PENALTY=0.801088
PRIMER_LEFT_24_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_24_SEQUENCE=GTCAACAGCTGCGAGAAAAA
PRIMER_LEFT_24=732,20
PRIMER_RIGHT_24=1685,20
PRIMER_LEFT_24_TM=60.111
PRIMER_RIGHT_24_TM=59.199
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.0000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=954
PRIMER_PAIR_25_PENALTY=0.911748
PRIMER_LEFT_25_PENALTY=0.110660
PRIMER_RIGHT_25_PENALTY=0.801088
PRIMER_LEFT_25_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_25_SEQUENCE=TCAACAGCTGCGAGAAAAAC
PRIMER_LEFT_25=731,20
PRIMER_RIGHT_25=1684,20
PRIMER_LEFT_25_TM=60.111
PRIMER_RIGHT_25_TM=59.199
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.2000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=954
PRIMER_PAIR_26_PENALTY=0.911748
PRIMER_LEFT_26_PENALTY=0.110660
PRIMER_RIGHT_26_PENALTY=0.801088
PRIMER_LEFT_26_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_26_SEQUENCE=GTCAACAGCTGCGAGAAAAA
PRIMER_LEFT_26=731,20
PRIMER_RIGHT_26=1685,20
PRIMER_LEFT_26_TM=60.111
PRIMER_RIGHT_26_TM=59.199
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.2000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=955
PRIMER_PAIR_27_PENALTY=1.162570
PRIMER_LEFT_27_PENALTY=0.110660
PRIMER_RIGHT_27_PENALTY=1.051910
PRIMER_LEFT_27_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_27_SEQUENCE=GTCAACAGCTGCGAGAAAAAC
PRIMER_LEFT_27=732,20
PRIMER_RIGHT_27=1685,21
PRIMER_LEFT_27_TM=60.111
PRIMER_RIGHT_27_TM=60.052
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.0000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=954
PRIMER_PAIR_28_PENALTY=1.162570
PRIMER_LEFT_28_PENALTY=0.110660
PRIMER_RIGHT_28_PENALTY=1.051910
PRIMER_LEFT_28_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_28_SEQUENCE=GTCAACAGCTGCGAGAAAAAC
PRIMER_LEFT_28=731,20
PRIMER_RIGHT_28=1685,21
PRIMER_LEFT_28_TM=60.111
PRIMER_RIGHT_28_TM=60.052
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=955
PRIMER_PAIR_29_PENALTY=1.199129
PRIMER_LEFT_29_PENALTY=0.110660
PRIMER_RIGHT_29_PENALTY=1.088469
PRIMER_LEFT_29_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_29_SEQUENCE=ACGGGGAAAGATGTAACGAC
PRIMER_LEFT_29=732,20
PRIMER_RIGHT_29=1622,20
PRIMER_LEFT_29_TM=60.111
PRIMER_RIGHT_29_TM=58.912
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.0000
PRIMER_RIGHT_29_END_STABILITY=7.8000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=891
PRIMER_PAIR_30_PENALTY=1.199129
PRIMER_LEFT_30_PENALTY=0.110660
PRIMER_RIGHT_30_PENALTY=1.088469
PRIMER_LEFT_30_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_30_SEQUENCE=ACGGGGAAAGATGTAACGAC
PRIMER_LEFT_30=731,20
PRIMER_RIGHT_30=1622,20
PRIMER_LEFT_30_TM=60.111
PRIMER_RIGHT_30_TM=58.912
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.2000
PRIMER_RIGHT_30_END_STABILITY=7.8000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=892
PRIMER_PAIR_31_PENALTY=1.277820
PRIMER_LEFT_31_PENALTY=0.110660
PRIMER_RIGHT_31_PENALTY=1.167160
PRIMER_LEFT_31_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_31_SEQUENCE=GCCGATTTTTGGAAAGCTAC
PRIMER_LEFT_31=732,20
PRIMER_RIGHT_31=1640,20
PRIMER_LEFT_31_TM=60.111
PRIMER_RIGHT_31_TM=58.833
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.0000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=909
PRIMER_PAIR_32_PENALTY=1.277820
PRIMER_LEFT_32_PENALTY=0.110660
PRIMER_RIGHT_32_PENALTY=1.167160
PRIMER_LEFT_32_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_32_SEQUENCE=GCCGATTTTTGGAAAGCTAC
PRIMER_LEFT_32=731,20
PRIMER_RIGHT_32=1640,20
PRIMER_LEFT_32_TM=60.111
PRIMER_RIGHT_32_TM=58.833
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.2000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=910
PRIMER_PAIR_33_PENALTY=1.293318
PRIMER_LEFT_33_PENALTY=0.110660
PRIMER_RIGHT_33_PENALTY=1.182658
PRIMER_LEFT_33_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_33_SEQUENCE=ACAGCTGCGAGAAAAACTTGA
PRIMER_LEFT_33=732,20
PRIMER_RIGHT_33=1681,21
PRIMER_LEFT_33_TM=60.111
PRIMER_RIGHT_33_TM=60.183
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.0000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=950
PRIMER_PAIR_34_PENALTY=1.293318
PRIMER_LEFT_34_PENALTY=0.110660
PRIMER_RIGHT_34_PENALTY=1.182658
PRIMER_LEFT_34_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_34_SEQUENCE=TCAACAGCTGCGAGAAAAACT
PRIMER_LEFT_34=732,20
PRIMER_RIGHT_34=1684,21
PRIMER_LEFT_34_TM=60.111
PRIMER_RIGHT_34_TM=60.183
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=953
PRIMER_PAIR_35_PENALTY=1.293318
PRIMER_LEFT_35_PENALTY=0.110660
PRIMER_RIGHT_35_PENALTY=1.182658
PRIMER_LEFT_35_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_35_SEQUENCE=ACAGCTGCGAGAAAAACTTGA
PRIMER_LEFT_35=731,20
PRIMER_RIGHT_35=1681,21
PRIMER_LEFT_35_TM=60.111
PRIMER_RIGHT_35_TM=60.183
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.2000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=951
PRIMER_PAIR_36_PENALTY=1.293318
PRIMER_LEFT_36_PENALTY=0.110660
PRIMER_RIGHT_36_PENALTY=1.182658
PRIMER_LEFT_36_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_36_SEQUENCE=TCAACAGCTGCGAGAAAAACT
PRIMER_LEFT_36=731,20
PRIMER_RIGHT_36=1684,21
PRIMER_LEFT_36_TM=60.111
PRIMER_RIGHT_36_TM=60.183
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.2000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=954
PRIMER_PAIR_37_PENALTY=1.302388
PRIMER_LEFT_37_PENALTY=0.110660
PRIMER_RIGHT_37_PENALTY=1.191728
PRIMER_LEFT_37_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_37_SEQUENCE=GGGATTGGAGTTGGGAAGAA
PRIMER_LEFT_37=732,20
PRIMER_RIGHT_37=1490,20
PRIMER_LEFT_37_TM=60.111
PRIMER_RIGHT_37_TM=61.192
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.0000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=759
PRIMER_PAIR_38_PENALTY=1.302388
PRIMER_LEFT_38_PENALTY=0.110660
PRIMER_RIGHT_38_PENALTY=1.191728
PRIMER_LEFT_38_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_38_SEQUENCE=GGGATTGGAGTTGGGAAGAA
PRIMER_LEFT_38=731,20
PRIMER_RIGHT_38=1490,20
PRIMER_LEFT_38_TM=60.111
PRIMER_RIGHT_38_TM=61.192
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.2000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=760
PRIMER_PAIR_39_PENALTY=1.317639
PRIMER_LEFT_39_PENALTY=0.110660
PRIMER_RIGHT_39_PENALTY=1.206979
PRIMER_LEFT_39_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_39_SEQUENCE=ATTTTGTTGAAGGAAGTGGGG
PRIMER_LEFT_39=732,20
PRIMER_RIGHT_39=1514,21
PRIMER_LEFT_39_TM=60.111
PRIMER_RIGHT_39_TM=60.207
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.0000
PRIMER_RIGHT_39_END_STABILITY=11.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=783
PRIMER_PAIR_40_PENALTY=1.317639
PRIMER_LEFT_40_PENALTY=0.110660
PRIMER_RIGHT_40_PENALTY=1.206979
PRIMER_LEFT_40_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_40_SEQUENCE=ATTTTGTTGAAGGAAGTGGGG
PRIMER_LEFT_40=731,20
PRIMER_RIGHT_40=1514,21
PRIMER_LEFT_40_TM=60.111
PRIMER_RIGHT_40_TM=60.207
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.2000
PRIMER_RIGHT_40_END_STABILITY=11.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=784
PRIMER_PAIR_41_PENALTY=1.323473
PRIMER_LEFT_41_PENALTY=0.110660
PRIMER_RIGHT_41_PENALTY=1.212813
PRIMER_LEFT_41_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_41_SEQUENCE=GAAAGCTACGGGGAAAGATG
PRIMER_LEFT_41=732,20
PRIMER_RIGHT_41=1629,20
PRIMER_LEFT_41_TM=60.111
PRIMER_RIGHT_41_TM=58.787
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.0000
PRIMER_RIGHT_41_END_STABILITY=6.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=898
PRIMER_PAIR_42_PENALTY=1.323473
PRIMER_LEFT_42_PENALTY=0.110660
PRIMER_RIGHT_42_PENALTY=1.212813
PRIMER_LEFT_42_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_42_SEQUENCE=GAAAGCTACGGGGAAAGATG
PRIMER_LEFT_42=731,20
PRIMER_RIGHT_42=1629,20
PRIMER_LEFT_42_TM=60.111
PRIMER_RIGHT_42_TM=58.787
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.2000
PRIMER_RIGHT_42_END_STABILITY=6.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=899
PRIMER_PAIR_43_PENALTY=1.360797
PRIMER_LEFT_43_PENALTY=0.110660
PRIMER_RIGHT_43_PENALTY=1.250137
PRIMER_LEFT_43_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_43_SEQUENCE=CTTGAATTACGCGTTGGAAGA
PRIMER_LEFT_43=732,20
PRIMER_RIGHT_43=1665,21
PRIMER_LEFT_43_TM=60.111
PRIMER_RIGHT_43_TM=60.250
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.0000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=934
PRIMER_PAIR_44_PENALTY=1.360797
PRIMER_LEFT_44_PENALTY=0.110660
PRIMER_RIGHT_44_PENALTY=1.250137
PRIMER_LEFT_44_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_44_SEQUENCE=CTTGAATTACGCGTTGGAAGA
PRIMER_LEFT_44=731,20
PRIMER_RIGHT_44=1665,21
PRIMER_LEFT_44_TM=60.111
PRIMER_RIGHT_44_TM=60.250
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.2000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=935
PRIMER_PAIR_45_PENALTY=1.373662
PRIMER_LEFT_45_PENALTY=0.110660
PRIMER_RIGHT_45_PENALTY=1.263002
PRIMER_LEFT_45_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_45_SEQUENCE=AAGATTTCGCCGATTTTTGG
PRIMER_LEFT_45=731,20
PRIMER_RIGHT_45=1648,20
PRIMER_LEFT_45_TM=60.111
PRIMER_RIGHT_45_TM=61.263
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.2000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=918
PRIMER_PAIR_46_PENALTY=1.382425
PRIMER_LEFT_46_PENALTY=1.323962
PRIMER_RIGHT_46_PENALTY=0.058463
PRIMER_LEFT_46_SEQUENCE=GATGACGCAAAACGGTAAAA
PRIMER_RIGHT_46_SEQUENCE=TTTTGTTGAAGGAAGTGGGG
PRIMER_LEFT_46=733,20
PRIMER_RIGHT_46=1513,20
PRIMER_LEFT_46_TM=58.676
PRIMER_RIGHT_46_TM=59.942
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.6000
PRIMER_RIGHT_46_END_STABILITY=11.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=781
PRIMER_PAIR_47_PENALTY=1.416877
PRIMER_LEFT_47_PENALTY=0.110660
PRIMER_RIGHT_47_PENALTY=1.306217
PRIMER_LEFT_47_SEQUENCE=TGATGACGCAAAACGGTAAA
PRIMER_RIGHT_47_SEQUENCE=GATTTTTGGAAAGCTACGGG
PRIMER_LEFT_47=732,20
PRIMER_RIGHT_47=1637,20
PRIMER_LEFT_47_TM=60.111
PRIMER_RIGHT_47_TM=58.694
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.0000
PRIMER_RIGHT_47_END_STABILITY=11.1000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=906
PRIMER_PAIR_48_PENALTY=1.416877
PRIMER_LEFT_48_PENALTY=0.110660
PRIMER_RIGHT_48_PENALTY=1.306217
PRIMER_LEFT_48_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_48_SEQUENCE=GATTTTTGGAAAGCTACGGG
PRIMER_LEFT_48=731,20
PRIMER_RIGHT_48=1637,20
PRIMER_LEFT_48_TM=60.111
PRIMER_RIGHT_48_TM=58.694
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.2000
PRIMER_RIGHT_48_END_STABILITY=11.1000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=907
PRIMER_PAIR_49_PENALTY=1.425004
PRIMER_LEFT_49_PENALTY=0.110660
PRIMER_RIGHT_49_PENALTY=1.314343
PRIMER_LEFT_49_SEQUENCE=TTGATGACGCAAAACGGTAA
PRIMER_RIGHT_49_SEQUENCE=GTTGGAAGATTTCGCCGATT
PRIMER_LEFT_49=731,20
PRIMER_RIGHT_49=1653,20
PRIMER_LEFT_49_TM=60.111
PRIMER_RIGHT_49_TM=61.314
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.2000
PRIMER_RIGHT_49_END_STABILITY=8.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.329_3_13992393_13992672_sf-2_ler-0_20842_45929_TRUE
SEQUENCE_TARGET=950,379
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTCTCNAAAANAAANGAACGNCTNAGATTAAAAACANGTTAGCGGAGCAGCTGACNTCATCAGGGGTAGAATTNTATTGATTTTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_12346697_12346796_hi-0_po-0_14559_277823_TRUE
SEQUENCE_TARGET=950,199
SEQUENCE_TEMPLATE=AAGACTTTTGATTCATTTATCAAACATTATCATGATACATTAATTANAGAGTAAAACTATGATCANGCCTTTAACAAAAACAAAATTATGATTAGTCATTAGTCAATAATTTTCTTGATTACGTTATTTTTTCCTTGAAACCAAACAGATCAGATCAATACACGAGATTTCGATAGGTAAAGAGGCGATACTCACGGATTAATATCTGAAAACGCAGATTATATNGGGACGGAGATNNTTNTCCGATCTAANTCGNTNGATTTANNGTAGTTGATGATTTATGTTCGTGAANTTGGAATATTTTTGGGTTTTAAAGAGAGACTTGAGGTTNAGGATAAAGTGTTTGAGTAAGAAGCGGATGCAGTATCAGGCCGNTACTAGGGGTCTTTTTATAGGCTGCAAGTCAACGAAATACCAATAATGTCCTTATTTGCGCGGGAATGATTTCTTCTTCTNTTTTTTTTTTCTAATATAAATAAAACAAGTAAACATTATCTTGATTCTTGAGGCTAGGGTTGGCAACTAGCAAACTGATTTCTTTAAGAAATTGGTGAGAAATGCTTCCCAATTTGGACGGTATTTGGAAACNAGTNATATATAAATATCTAATTATCTANTTGTTTTCGGTATGGGATATGTGAGTGGTACATTGTTTTAAATTATATTGGTTTATTAGCTCAAGCAATAATGTAGTTTCACNATCACTAGTAATTNCTTTAGGCCACAAGATCTAAGATTTCACATACTATTAGTCTTATTTTACCANATTTTNGTATGGTCAAACAACTAGTCTTGATAGTTNTAATTTGAGCTCCTTGATTCNAATNCCCATTAATCACANAATTCNTATTCACTTATCAAATATTGGATTTACACACTCATTTCATCGTATGNATGTTCTCATTGACGTCGATNCGTTCACATGTNAACATNTNATATTCGTCTTCTCTCCTACCATCAAAATTTANCCCAACATATNCATAGAATNGATAATTAGTTGNTATGATGAGTATAACNAAATTTATNTTTAAGACTTCTATTTTTAACTAATATTATGTNTATAACATANAATNNNNNNNNNNNNNTATATGAGATGACANNNNAATTTGACAGTATNTTCAGATAAAANTACAAGTCTTATTTGTATACTTGCCAGTTGCCACTTGTAGGATAAGCCAAAATTAGTCNNTTTTTCTATCTTGGTNTTCATATTTCAGGCCACCNNAAAAGAAGAAAGCAAATAAANAGTCAAATTAGACTCTTTNGTAAAATAGACCTCAAATGTATATTNNNNCTNTCTCTCTCTTATCGGTTATCGTCGGTTCGCCACCATCACATACCTTTCTAGTAACGTAAGGNTGACCAAATTAAGCTTCTTGATCGAATTTTGCTTNCTCTTAAACACAGAAATANNACCATATATCTCNTATATGCACAAAAAGTATANCTGTATGACTAAAACTCTTGANATAAAACACCGANCCANNNNNNNNNNNNNNNNNNNNNCCTATATAAATTANGTAAAATGGAATTAAGGTCTCAGTTGTACAAAGGAATGAATAAGACATTAGATTGAAAATATTAGTTTGNCAGGTGATTCAACCCGCCATATGGGATAACAAAGAGTGAATACATATNCAAATTCGATTAACATTCAACAGTGCAATTAATATGACAGATCANATGTTTTAACTAGAGAAATTTAAGTCAAATATATTCCAACTTCTAATTGTTAAAAATGAATCATTGGGTACCTGACTGAACCNAANAATTTCACATGTTTGGATTTNATNATTTNNAANAGAAGGTTAAAACAAACTCAGGNAGAGGNGACTTTCATATTTTTCNCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.720172
PRIMER_LEFT_0_PENALTY=0.391374
PRIMER_RIGHT_0_PENALTY=1.328798
PRIMER_LEFT_0_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_0_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_0=392,20
PRIMER_RIGHT_0=1341,20
PRIMER_LEFT_0_TM=59.609
PRIMER_RIGHT_0_TM=61.329
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.0000
PRIMER_RIGHT_0_END_STABILITY=10.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=950
PRIMER_PAIR_1_PENALTY=2.199566
PRIMER_LEFT_1_PENALTY=0.391374
PRIMER_RIGHT_1_PENALTY=1.808192
PRIMER_LEFT_1_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_1_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_1=392,20
PRIMER_RIGHT_1=1321,21
PRIMER_LEFT_1_TM=59.609
PRIMER_RIGHT_1_TM=59.192
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=930
PRIMER_PAIR_2_PENALTY=2.404775
PRIMER_LEFT_2_PENALTY=0.391374
PRIMER_RIGHT_2_PENALTY=2.013401
PRIMER_LEFT_2_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_2_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_2=392,20
PRIMER_RIGHT_2=1322,22
PRIMER_LEFT_2_TM=59.609
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=931
PRIMER_PAIR_3_PENALTY=2.409835
PRIMER_LEFT_3_PENALTY=1.081037
PRIMER_RIGHT_3_PENALTY=1.328798
PRIMER_LEFT_3_SEQUENCE=CGGTATGGGATATGTGAGTGG
PRIMER_RIGHT_3_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_3=624,21
PRIMER_RIGHT_3=1341,20
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=61.329
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=718
PRIMER_PAIR_4_PENALTY=2.410334
PRIMER_LEFT_4_PENALTY=1.081536
PRIMER_RIGHT_4_PENALTY=1.328798
PRIMER_LEFT_4_SEQUENCE=GATTCTTGAGGCTAGGGTTGG
PRIMER_RIGHT_4_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_4=498,21
PRIMER_RIGHT_4=1341,20
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=61.329
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=844
PRIMER_PAIR_5_PENALTY=2.432014
PRIMER_LEFT_5_PENALTY=1.103216
PRIMER_RIGHT_5_PENALTY=1.328798
PRIMER_LEFT_5_SEQUENCE=ATAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_5_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_5=391,21
PRIMER_RIGHT_5=1341,20
PRIMER_LEFT_5_TM=59.897
PRIMER_RIGHT_5_TM=61.329
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.0000
PRIMER_RIGHT_5_END_STABILITY=10.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=951
PRIMER_PAIR_6_PENALTY=2.500304
PRIMER_LEFT_6_PENALTY=0.391374
PRIMER_RIGHT_6_PENALTY=2.108929
PRIMER_LEFT_6_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_6_SEQUENCE=CCGACGATAACCGATAAGAGAG
PRIMER_LEFT_6=392,20
PRIMER_RIGHT_6=1321,22
PRIMER_LEFT_6_TM=59.609
PRIMER_RIGHT_6_TM=60.109
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.0000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=930
PRIMER_PAIR_7_PENALTY=2.562342
PRIMER_LEFT_7_PENALTY=1.233544
PRIMER_RIGHT_7_PENALTY=1.328798
PRIMER_LEFT_7_SEQUENCE=GCTGCAAGTCAACGAAATACC
PRIMER_RIGHT_7_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_7=395,21
PRIMER_RIGHT_7=1341,20
PRIMER_LEFT_7_TM=59.766
PRIMER_RIGHT_7_TM=61.329
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.8000
PRIMER_RIGHT_7_END_STABILITY=10.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=947
PRIMER_PAIR_8_PENALTY=2.562342
PRIMER_LEFT_8_PENALTY=1.233544
PRIMER_RIGHT_8_PENALTY=1.328798
PRIMER_LEFT_8_SEQUENCE=GGCTGCAAGTCAACGAAATAC
PRIMER_RIGHT_8_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_8=394,21
PRIMER_RIGHT_8=1341,20
PRIMER_LEFT_8_TM=59.766
PRIMER_RIGHT_8_TM=61.329
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=5.6000
PRIMER_RIGHT_8_END_STABILITY=10.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=948
PRIMER_PAIR_9_PENALTY=2.612079
PRIMER_LEFT_9_PENALTY=1.283281
PRIMER_RIGHT_9_PENALTY=1.328798
PRIMER_LEFT_9_SEQUENCE=GCGGGAATGATTTCTTCTTC
PRIMER_RIGHT_9_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_9=434,20
PRIMER_RIGHT_9=1341,20
PRIMER_LEFT_9_TM=58.717
PRIMER_RIGHT_9_TM=61.329
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=10.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=908
PRIMER_PAIR_10_PENALTY=2.649275
PRIMER_LEFT_10_PENALTY=1.320478
PRIMER_RIGHT_10_PENALTY=1.328798
PRIMER_LEFT_10_SEQUENCE=GCGGGAATGATTTCTTCTTCT
PRIMER_RIGHT_10_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_10=434,21
PRIMER_RIGHT_10=1341,20
PRIMER_LEFT_10_TM=59.680
PRIMER_RIGHT_10_TM=61.329
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=10.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=908
PRIMER_PAIR_11_PENALTY=2.723316
PRIMER_LEFT_11_PENALTY=0.391374
PRIMER_RIGHT_11_PENALTY=2.331942
PRIMER_LEFT_11_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_11_SEQUENCE=GAACCGACGATAACCGATAAGA
PRIMER_LEFT_11=392,20
PRIMER_RIGHT_11=1324,22
PRIMER_LEFT_11_TM=59.609
PRIMER_RIGHT_11_TM=60.332
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.0000
PRIMER_RIGHT_11_END_STABILITY=6.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=933
PRIMER_PAIR_12_PENALTY=2.760382
PRIMER_LEFT_12_PENALTY=1.431584
PRIMER_RIGHT_12_PENALTY=1.328798
PRIMER_LEFT_12_SEQUENCE=CGCGGGAATGATTTCTTCTT
PRIMER_RIGHT_12_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_12=433,20
PRIMER_RIGHT_12=1341,20
PRIMER_LEFT_12_TM=61.432
PRIMER_RIGHT_12_TM=61.329
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=10.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=909
PRIMER_PAIR_13_PENALTY=2.797965
PRIMER_LEFT_13_PENALTY=0.391374
PRIMER_RIGHT_13_PENALTY=2.406591
PRIMER_LEFT_13_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_13_SEQUENCE=AACCGACGATAACCGATAAGA
PRIMER_LEFT_13=392,20
PRIMER_RIGHT_13=1323,21
PRIMER_LEFT_13_TM=59.609
PRIMER_RIGHT_13_TM=58.593
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.0000
PRIMER_RIGHT_13_END_STABILITY=6.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=932
PRIMER_PAIR_14_PENALTY=2.797965
PRIMER_LEFT_14_PENALTY=0.391374
PRIMER_RIGHT_14_PENALTY=2.406591
PRIMER_LEFT_14_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_14_SEQUENCE=GAACCGACGATAACCGATAAG
PRIMER_LEFT_14=392,20
PRIMER_RIGHT_14=1324,21
PRIMER_LEFT_14_TM=59.609
PRIMER_RIGHT_14_TM=58.593
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.0000
PRIMER_RIGHT_14_END_STABILITY=5.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=933
PRIMER_PAIR_15_PENALTY=2.799905
PRIMER_LEFT_15_PENALTY=1.471107
PRIMER_RIGHT_15_PENALTY=1.328798
PRIMER_LEFT_15_SEQUENCE=ATAGGCTGCAAGTCAACGAA
PRIMER_RIGHT_15_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_15=391,20
PRIMER_RIGHT_15=1341,20
PRIMER_LEFT_15_TM=58.529
PRIMER_RIGHT_15_TM=61.329
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=10.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=951
PRIMER_PAIR_16_PENALTY=2.868408
PRIMER_LEFT_16_PENALTY=0.391374
PRIMER_RIGHT_16_PENALTY=2.477034
PRIMER_LEFT_16_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_16_SEQUENCE=AACCGACGATAACCGATAAGAG
PRIMER_LEFT_16=392,20
PRIMER_RIGHT_16=1323,22
PRIMER_LEFT_16_TM=59.609
PRIMER_RIGHT_16_TM=59.523
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=932
PRIMER_PAIR_17_PENALTY=2.889229
PRIMER_LEFT_17_PENALTY=1.081037
PRIMER_RIGHT_17_PENALTY=1.808192
PRIMER_LEFT_17_SEQUENCE=CGGTATGGGATATGTGAGTGG
PRIMER_RIGHT_17_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_17=624,21
PRIMER_RIGHT_17=1321,21
PRIMER_LEFT_17_TM=60.081
PRIMER_RIGHT_17_TM=59.192
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=698
PRIMER_PAIR_18_PENALTY=2.889728
PRIMER_LEFT_18_PENALTY=1.081536
PRIMER_RIGHT_18_PENALTY=1.808192
PRIMER_LEFT_18_SEQUENCE=GATTCTTGAGGCTAGGGTTGG
PRIMER_RIGHT_18_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_18=498,21
PRIMER_RIGHT_18=1321,21
PRIMER_LEFT_18_TM=60.082
PRIMER_RIGHT_18_TM=59.192
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=824
PRIMER_PAIR_19_PENALTY=2.911408
PRIMER_LEFT_19_PENALTY=1.103216
PRIMER_RIGHT_19_PENALTY=1.808192
PRIMER_LEFT_19_SEQUENCE=ATAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_19_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_19=391,21
PRIMER_RIGHT_19=1321,21
PRIMER_LEFT_19_TM=59.897
PRIMER_RIGHT_19_TM=59.192
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.0000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=931
PRIMER_PAIR_20_PENALTY=2.994343
PRIMER_LEFT_20_PENALTY=1.665545
PRIMER_RIGHT_20_PENALTY=1.328798
PRIMER_LEFT_20_SEQUENCE=CAATTTGGACGGTATTTGGA
PRIMER_RIGHT_20_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_20=565,20
PRIMER_RIGHT_20=1341,20
PRIMER_LEFT_20_TM=58.334
PRIMER_RIGHT_20_TM=61.329
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=10.2000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=777
PRIMER_PAIR_21_PENALTY=3.007081
PRIMER_LEFT_21_PENALTY=1.678283
PRIMER_RIGHT_21_PENALTY=1.328798
PRIMER_LEFT_21_SEQUENCE=TGATTCTTGAGGCTAGGGTTG
PRIMER_RIGHT_21_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_21=497,21
PRIMER_RIGHT_21=1341,20
PRIMER_LEFT_21_TM=59.322
PRIMER_RIGHT_21_TM=61.329
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=10.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=845
PRIMER_PAIR_22_PENALTY=3.041736
PRIMER_LEFT_22_PENALTY=1.233544
PRIMER_RIGHT_22_PENALTY=1.808192
PRIMER_LEFT_22_SEQUENCE=GCTGCAAGTCAACGAAATACC
PRIMER_RIGHT_22_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_22=395,21
PRIMER_RIGHT_22=1321,21
PRIMER_LEFT_22_TM=59.766
PRIMER_RIGHT_22_TM=59.192
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.8000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=927
PRIMER_PAIR_23_PENALTY=3.041736
PRIMER_LEFT_23_PENALTY=1.233544
PRIMER_RIGHT_23_PENALTY=1.808192
PRIMER_LEFT_23_SEQUENCE=GGCTGCAAGTCAACGAAATAC
PRIMER_RIGHT_23_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_23=394,21
PRIMER_RIGHT_23=1321,21
PRIMER_LEFT_23_TM=59.766
PRIMER_RIGHT_23_TM=59.192
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=5.6000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=928
PRIMER_PAIR_24_PENALTY=3.053201
PRIMER_LEFT_24_PENALTY=0.391374
PRIMER_RIGHT_24_PENALTY=2.661827
PRIMER_LEFT_24_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_24_SEQUENCE=CCGACGATAACCGATAAGAG
PRIMER_LEFT_24=392,20
PRIMER_RIGHT_24=1321,20
PRIMER_LEFT_24_TM=59.609
PRIMER_RIGHT_24_TM=57.338
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.0000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=930
PRIMER_PAIR_25_PENALTY=3.062342
PRIMER_LEFT_25_PENALTY=1.733544
PRIMER_RIGHT_25_PENALTY=1.328798
PRIMER_LEFT_25_SEQUENCE=ATTCTTGAGGCTAGGGTTGG
PRIMER_RIGHT_25_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_25=499,20
PRIMER_RIGHT_25=1341,20
PRIMER_LEFT_25_TM=58.266
PRIMER_RIGHT_25_TM=61.329
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=10.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=843
PRIMER_PAIR_26_PENALTY=3.091473
PRIMER_LEFT_26_PENALTY=1.283281
PRIMER_RIGHT_26_PENALTY=1.808192
PRIMER_LEFT_26_SEQUENCE=GCGGGAATGATTTCTTCTTC
PRIMER_RIGHT_26_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_26=434,20
PRIMER_RIGHT_26=1321,21
PRIMER_LEFT_26_TM=58.717
PRIMER_RIGHT_26_TM=59.192
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=888
PRIMER_PAIR_27_PENALTY=3.094438
PRIMER_LEFT_27_PENALTY=1.081037
PRIMER_RIGHT_27_PENALTY=2.013401
PRIMER_LEFT_27_SEQUENCE=CGGTATGGGATATGTGAGTGG
PRIMER_RIGHT_27_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_27=624,21
PRIMER_RIGHT_27=1322,22
PRIMER_LEFT_27_TM=60.081
PRIMER_RIGHT_27_TM=59.987
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=45.455
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=699
PRIMER_PAIR_28_PENALTY=3.094937
PRIMER_LEFT_28_PENALTY=1.081536
PRIMER_RIGHT_28_PENALTY=2.013401
PRIMER_LEFT_28_SEQUENCE=GATTCTTGAGGCTAGGGTTGG
PRIMER_RIGHT_28_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_28=498,21
PRIMER_RIGHT_28=1322,22
PRIMER_LEFT_28_TM=60.082
PRIMER_RIGHT_28_TM=59.987
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=825
PRIMER_PAIR_29_PENALTY=3.116617
PRIMER_LEFT_29_PENALTY=1.103216
PRIMER_RIGHT_29_PENALTY=2.013401
PRIMER_LEFT_29_SEQUENCE=ATAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_29_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_29=391,21
PRIMER_RIGHT_29=1322,22
PRIMER_LEFT_29_TM=59.897
PRIMER_RIGHT_29_TM=59.987
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.0000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=932
PRIMER_PAIR_30_PENALTY=3.165505
PRIMER_LEFT_30_PENALTY=0.391374
PRIMER_RIGHT_30_PENALTY=2.774130
PRIMER_LEFT_30_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_30_SEQUENCE=ACCGACGATAACCGATAAGAG
PRIMER_LEFT_30=392,20
PRIMER_RIGHT_30=1322,21
PRIMER_LEFT_30_TM=59.609
PRIMER_RIGHT_30_TM=58.226
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.0000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=931
PRIMER_PAIR_31_PENALTY=3.189966
PRIMER_LEFT_31_PENALTY=1.081037
PRIMER_RIGHT_31_PENALTY=2.108929
PRIMER_LEFT_31_SEQUENCE=CGGTATGGGATATGTGAGTGG
PRIMER_RIGHT_31_SEQUENCE=CCGACGATAACCGATAAGAGAG
PRIMER_LEFT_31=624,21
PRIMER_RIGHT_31=1321,22
PRIMER_LEFT_31_TM=60.081
PRIMER_RIGHT_31_TM=60.109
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=698
PRIMER_PAIR_32_PENALTY=3.190466
PRIMER_LEFT_32_PENALTY=1.081536
PRIMER_RIGHT_32_PENALTY=2.108929
PRIMER_LEFT_32_SEQUENCE=GATTCTTGAGGCTAGGGTTGG
PRIMER_RIGHT_32_SEQUENCE=CCGACGATAACCGATAAGAGAG
PRIMER_LEFT_32=498,21
PRIMER_RIGHT_32=1321,22
PRIMER_LEFT_32_TM=60.082
PRIMER_RIGHT_32_TM=60.109
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=824
PRIMER_PAIR_33_PENALTY=3.207118
PRIMER_LEFT_33_PENALTY=0.391374
PRIMER_RIGHT_33_PENALTY=2.815743
PRIMER_LEFT_33_SEQUENCE=TAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_33_SEQUENCE=ACCGACGATAACCGATAAGA
PRIMER_LEFT_33=392,20
PRIMER_RIGHT_33=1322,20
PRIMER_LEFT_33_TM=59.609
PRIMER_RIGHT_33_TM=57.184
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.0000
PRIMER_RIGHT_33_END_STABILITY=6.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=3.212146
PRIMER_LEFT_34_PENALTY=1.103216
PRIMER_RIGHT_34_PENALTY=2.108929
PRIMER_LEFT_34_SEQUENCE=ATAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_34_SEQUENCE=CCGACGATAACCGATAAGAGAG
PRIMER_LEFT_34=391,21
PRIMER_RIGHT_34=1321,22
PRIMER_LEFT_34_TM=59.897
PRIMER_RIGHT_34_TM=60.109
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.0000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=931
PRIMER_PAIR_35_PENALTY=3.246945
PRIMER_LEFT_35_PENALTY=1.233544
PRIMER_RIGHT_35_PENALTY=2.013401
PRIMER_LEFT_35_SEQUENCE=GCTGCAAGTCAACGAAATACC
PRIMER_RIGHT_35_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_35=395,21
PRIMER_RIGHT_35=1322,22
PRIMER_LEFT_35_TM=59.766
PRIMER_RIGHT_35_TM=59.987
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.8000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=928
PRIMER_PAIR_36_PENALTY=3.246945
PRIMER_LEFT_36_PENALTY=1.233544
PRIMER_RIGHT_36_PENALTY=2.013401
PRIMER_LEFT_36_SEQUENCE=GGCTGCAAGTCAACGAAATAC
PRIMER_RIGHT_36_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_36=394,21
PRIMER_RIGHT_36=1322,22
PRIMER_LEFT_36_TM=59.766
PRIMER_RIGHT_36_TM=59.987
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.6000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=929
PRIMER_PAIR_37_PENALTY=3.279299
PRIMER_LEFT_37_PENALTY=1.471107
PRIMER_RIGHT_37_PENALTY=1.808192
PRIMER_LEFT_37_SEQUENCE=ATAGGCTGCAAGTCAACGAA
PRIMER_RIGHT_37_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_37=391,20
PRIMER_RIGHT_37=1321,21
PRIMER_LEFT_37_TM=58.529
PRIMER_RIGHT_37_TM=59.192
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=931
PRIMER_PAIR_38_PENALTY=3.296682
PRIMER_LEFT_38_PENALTY=1.283281
PRIMER_RIGHT_38_PENALTY=2.013401
PRIMER_LEFT_38_SEQUENCE=GCGGGAATGATTTCTTCTTC
PRIMER_RIGHT_38_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_38=434,20
PRIMER_RIGHT_38=1322,22
PRIMER_LEFT_38_TM=58.717
PRIMER_RIGHT_38_TM=59.987
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=889
PRIMER_PAIR_39_PENALTY=3.342474
PRIMER_LEFT_39_PENALTY=1.233544
PRIMER_RIGHT_39_PENALTY=2.108929
PRIMER_LEFT_39_SEQUENCE=GCTGCAAGTCAACGAAATACC
PRIMER_RIGHT_39_SEQUENCE=CCGACGATAACCGATAAGAGAG
PRIMER_LEFT_39=395,21
PRIMER_RIGHT_39=1321,22
PRIMER_LEFT_39_TM=59.766
PRIMER_RIGHT_39_TM=60.109
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.8000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=927
PRIMER_PAIR_40_PENALTY=3.342474
PRIMER_LEFT_40_PENALTY=1.233544
PRIMER_RIGHT_40_PENALTY=2.108929
PRIMER_LEFT_40_SEQUENCE=GGCTGCAAGTCAACGAAATAC
PRIMER_RIGHT_40_SEQUENCE=CCGACGATAACCGATAAGAGAG
PRIMER_LEFT_40=394,21
PRIMER_RIGHT_40=1321,22
PRIMER_LEFT_40_TM=59.766
PRIMER_RIGHT_40_TM=60.109
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.6000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=928
PRIMER_PAIR_41_PENALTY=3.392211
PRIMER_LEFT_41_PENALTY=1.283281
PRIMER_RIGHT_41_PENALTY=2.108929
PRIMER_LEFT_41_SEQUENCE=GCGGGAATGATTTCTTCTTC
PRIMER_RIGHT_41_SEQUENCE=CCGACGATAACCGATAAGAGAG
PRIMER_LEFT_41=434,20
PRIMER_RIGHT_41=1321,22
PRIMER_LEFT_41_TM=58.717
PRIMER_RIGHT_41_TM=60.109
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=888
PRIMER_PAIR_42_PENALTY=3.412979
PRIMER_LEFT_42_PENALTY=1.081037
PRIMER_RIGHT_42_PENALTY=2.331942
PRIMER_LEFT_42_SEQUENCE=CGGTATGGGATATGTGAGTGG
PRIMER_RIGHT_42_SEQUENCE=GAACCGACGATAACCGATAAGA
PRIMER_LEFT_42=624,21
PRIMER_RIGHT_42=1324,22
PRIMER_LEFT_42_TM=60.081
PRIMER_RIGHT_42_TM=60.332
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.0000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=701
PRIMER_PAIR_43_PENALTY=3.418179
PRIMER_LEFT_43_PENALTY=2.089381
PRIMER_RIGHT_43_PENALTY=1.328798
PRIMER_LEFT_43_SEQUENCE=TATAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_43_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_43=390,22
PRIMER_RIGHT_43=1341,20
PRIMER_LEFT_43_TM=59.911
PRIMER_RIGHT_43_TM=61.329
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.0000
PRIMER_RIGHT_43_END_STABILITY=10.2000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=952
PRIMER_PAIR_44_PENALTY=3.418179
PRIMER_LEFT_44_PENALTY=2.089381
PRIMER_RIGHT_44_PENALTY=1.328798
PRIMER_LEFT_44_SEQUENCE=TTATAGGCTGCAAGTCAACGAA
PRIMER_RIGHT_44_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_44=389,22
PRIMER_RIGHT_44=1341,20
PRIMER_LEFT_44_TM=59.911
PRIMER_RIGHT_44_TM=61.329
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.4000
PRIMER_RIGHT_44_END_STABILITY=10.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=953
PRIMER_PAIR_45_PENALTY=3.418179
PRIMER_LEFT_45_PENALTY=2.089381
PRIMER_RIGHT_45_PENALTY=1.328798
PRIMER_LEFT_45_SEQUENCE=TTTATAGGCTGCAAGTCAACGA
PRIMER_RIGHT_45_SEQUENCE=AGGTATGTGATGGTGGCGAA
PRIMER_LEFT_45=388,22
PRIMER_RIGHT_45=1341,20
PRIMER_LEFT_45_TM=59.911
PRIMER_RIGHT_45_TM=61.329
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.4000
PRIMER_RIGHT_45_END_STABILITY=10.2000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=954
PRIMER_PAIR_46_PENALTY=3.435158
PRIMER_LEFT_46_PENALTY=1.103216
PRIMER_RIGHT_46_PENALTY=2.331942
PRIMER_LEFT_46_SEQUENCE=ATAGGCTGCAAGTCAACGAAA
PRIMER_RIGHT_46_SEQUENCE=GAACCGACGATAACCGATAAGA
PRIMER_LEFT_46=391,21
PRIMER_RIGHT_46=1324,22
PRIMER_LEFT_46_TM=59.897
PRIMER_RIGHT_46_TM=60.332
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.0000
PRIMER_RIGHT_46_END_STABILITY=6.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=934
PRIMER_PAIR_47_PENALTY=3.473737
PRIMER_LEFT_47_PENALTY=1.665545
PRIMER_RIGHT_47_PENALTY=1.808192
PRIMER_LEFT_47_SEQUENCE=CAATTTGGACGGTATTTGGA
PRIMER_RIGHT_47_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_47=565,20
PRIMER_RIGHT_47=1321,21
PRIMER_LEFT_47_TM=58.334
PRIMER_RIGHT_47_TM=59.192
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=757
PRIMER_PAIR_48_PENALTY=3.484508
PRIMER_LEFT_48_PENALTY=1.471107
PRIMER_RIGHT_48_PENALTY=2.013401
PRIMER_LEFT_48_SEQUENCE=ATAGGCTGCAAGTCAACGAA
PRIMER_RIGHT_48_SEQUENCE=ACCGACGATAACCGATAAGAGA
PRIMER_LEFT_48=391,20
PRIMER_RIGHT_48=1322,22
PRIMER_LEFT_48_TM=58.529
PRIMER_RIGHT_48_TM=59.987
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=932
PRIMER_PAIR_49_PENALTY=3.486475
PRIMER_LEFT_49_PENALTY=1.678283
PRIMER_RIGHT_49_PENALTY=1.808192
PRIMER_LEFT_49_SEQUENCE=TGATTCTTGAGGCTAGGGTTG
PRIMER_RIGHT_49_SEQUENCE=CCGACGATAACCGATAAGAGA
PRIMER_LEFT_49=497,21
PRIMER_RIGHT_49=1321,21
PRIMER_LEFT_49_TM=59.322
PRIMER_RIGHT_49_TM=59.192
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=825
=
SEQUENCE_ID=MAGIC.446_5_20809496_20810078_sf-2_can-0_659335_3811_TRUE
SEQUENCE_TARGET=950,682
SEQUENCE_TEMPLATE=AACTTCATTAATGGCTGCACGTTAGATCATTGCTATTTTTCTTATGTTTTGATAGTGTCTAATTAGNTGAATGTATGAGCCAAGATGAGAAATACAATAGCGTATCTACNTGTTTATCTCCTGTGTCAAGCCTGACATCATTATTNTGCAGGTGAAGGAATCAAAGGANATGGCTATGAGTGTTCTAAATTCTTACCGAACTTCTGATCCGCTTGATGNAAGTATGCAGACTGAAGAGTCTGACAGGCCACTGCCCTTCATTTCTCTTATGGAGTTTGTAAGCAAAATTTATCAGGTTGAGAATTACTACCATATAAAANTTTATCAAGAGTTCTTTATCTTTGTTTTTTTTTCTGTTATATTTCTCAAAAAATCTTNGAAAATGAGTTTGCTTGGTATCTTGAAGTTGAGTTCTGTTCATATGTTTTTTGGTACCTTATTTTGCTTTTCATAGCACATAGTGAGCATTCAGAATCCATCTCGATCATATCNCATTTTTATACTACATTCCTGGTTTTGTGTTTTTTGATATTTACAACCTTTTGCAGAAGGAACCTGAGCTACTCTCCGGGAATGATGTCTTGTGGACTTTTGTGAATTTTGCTGGAGAGGACCACACAAATTTTAAAACTCTTGTGGCTTTCTTGGAAATGCTGTGTACCTTGGTATGAGAAATCTTTATATTGNTATTTTGGTGGGACCTTATTTNCTTGGTNACTCATTGAGTTTCCTTGCTGGCATCTTTATTTAATTTCAGGCTTCCACTCAGGAAGGTGCTTCTAAAGTTTATGAGTTGCTCCGGGGTACATCATTTCGCAGTATAGGATGGCCCACTTTGTTTGATTGTATAAGNATTTACGATGAGAAGTTCAAGCAGTCTCTTCAAACTGCTGGAGCCATGATGCCAGAGTTCCTGGAGGGCGATGCAAAAGCACTTGTCGCTTATCTGAATGTACTTCAGAAGGTTATATAACTCTATTCCAAATATATTCTGTTTCAANTTTCATTCCACGTTTAATATTTTTATATGGTGCTTTTTATCTAAGACAATTTCAGCTTGTATTCCTAGGAACTGAACTTTAACAGCGATGTGGNTCTAGTTGACTGTANAAATTTTTTATATGGNCAACAAGTTTATTTTTCATATGCTGCAGGTTGTTGAAAATGGTAATCCCACTGAGAGGAAAAACTGGTTTCCTGATATCGAACCATTTTTTAAGCTTCTGGGTTACGAAAACATCCCTCCATATCTGAAGGTCAGTGGCGTACTGGAGATTCTTCCCCTTACACTTTGGACTCGCAAATGCTATGTGGAAAGCATCTGGTATCGGTATCTATATAACTTGTGTTCATTGTAACTTTATTTTTCTGCAGGGTGCTCTTAGAAAGACCATTGCTGCCTTTGTTAATGTCTTTCCAGAAATGAGGGACTCTATCTGGGCTTTTCTTGAGCAGTATGATCTGCCTGTTGTTGTGGGATCACAAGTTGGAAAGAGTGACCAATCTTCTCAGGTAATTGCATGACTTCGAGNGGATAGTTATTAGTAGATNTTTTAATTCTACTTCGTTAGTTAATNTNNNCNTTTNTTANTNANNCNTTANNANNNANNNTTNTNNTNNTGCATNNANNNTTNNNANCATTNTTTNNTTANGNAAAGNNNNNGTTNGTNNCANANANNATTNNTANNATTNNTNTNNNTTNTGNANNAANTTNATNTGNTNANANANNNANTTNNNNNTATTNNNCTNANTNCTNNNANAACNNNNTGTGNNATGNNNNNTNNAGNTNNNNCNTNNATNTCACNNGTNNTNTNATNNNGNTTNTGCTNNNNNNNNNNNNNNNNATNTTCANNGTANCTNNAAAACNNAATNTCACNTNTNGTTTNNATGCNGNTNNNNNNTNTGCNNTTNNAGNNNNNNNNNGTGGNAGNANNNNNAGNNNNGTATNCTTCAANNATANNGTNNCNTNNTCNTNTANATGNTCTNNNTGNTNNNNANNNNGANNTNAATGANNGTGGNNGNANGNAGNGNTANCCTNANANTNTNGTNNCTNTNTATNANGCTNNNGCNTNNNTNNTCNTGGNCNNANGTNNTNANGTANATNNTTGTANNTTTNTNNGNANCTTNNGNTTNGTTNATGNTCNNNTNTTNNNANNTNTNNCTNNGNNGNNNTATTNNNATCNNNNTNAGNNGTNNNNNTTGNTTNTNGCATGNNTTCNACNTTNTCANANGNNANNTNCNGNNTCTGTNNNNAGNAATTNNNTNTNNCNNGCNNNNTNCAANATNNNNNNNNNTTTNNNNNANANNTAAGNNCNTGNTTCTNNTTTTTNAANNNCCNATNNTGGNNCNNNCNNNTNTAAACTNNNTNNNTTTNCATTNAGGNCNCTNTNTANNTTNNANACNNGNNTANNNTNNTNCTNNNTTNCACTNCANNANNTTNNNCATNTANNATATTNANGNNGANGANCTTGATNNNTTTNCTGNNNNTNCACNNNTTCNGNTNTNNCNTGNANNATNTTCANNGNANNNGNANCTNNNNNTNATNGANTTACTNANNNTNCNNATTTNNNNTGTNNCNTTGN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_11038891_11039099_wil-2_edi-0_262998_993603_TRUE
SEQUENCE_TARGET=950,308
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_5_11073448_11074054_ws-0_ct-1_37329_106760_TRUE
SEQUENCE_TARGET=950,706
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_3144017_3144113_ws-0_can-0_324541_1140_TRUE
SEQUENCE_TARGET=950,196
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNGCAANTNCTTTNTAGAATCNAANAGGAAATTNNANACTGTNATTTGCGATAAAGATGAGTGAGAGAAACTNGTTCTTAGATTTNCTTTTATAACGAGAAGTTTCCTTTGGAATCAGAGCAGTTGCATCACGAAGTGGGGANATCCACGACATGGAGATAACCGTCGCNNNNNNNNNNNNNANGAACTGCCCNCATCNTCGACAAAACCGTCNGAGATCNACTCCNGGTAACCGTTANAGCAGATCCAAAANTAANTGTGTATCGCATNCACGATCGCCTTTGCAATCGNNNCCATTNCNTTNNNNNNNATNTGATCCTATGAGATTTNTTTTTTTATATAAAATGATACAAAAGATTAGATTTGAATCTAAATAAACATTTTTCATAAAAACATTTGAGTATNANAANNNCNTCAGCTTTTNNTCTACTGTAAAAAGCTTCTAAAACATTCTCANCCTTCTCCATATTTTNNAGTTCNATGATGATGTATCTTTGTGGAAGANAAACCACCGAACNAGTAATCTNGACGTTAGTACANCTNTNTACCCACAAAGGAGNGAATNCAAAATAGACTACTTTATACTCACNTCNAGANTTAAGATTTGATTGNTNTTCTACTCACACCAAANCAAAAACCAAAGATCATAAAGATCAGATTATTCGTCCTTTNNTCGAANCTTATANATTTATTATTTTATTGANCANTATCTGAAATAANAACAACATNATCANATATTTAACNANAAAAGGTTAATAATAAGGATTNNNNCTGGGGACAAANCCAATCATNCTTGAACGCCATTTCATATGTGTTCTCATTNAAGTANAAANTCGTTACCAATGANAACAAATTTAGCAATGAAACTCATA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_6011204_6011357_wil-2_edi-0_165325_301243_TRUE
SEQUENCE_TARGET=950,253
SEQUENCE_TEMPLATE=AGATATACAATCACACGCATAAAACTCATTAAAATTTCAAAAGAAATAAGGATACTTAAATGAAGAACTTAGAGAAAATAGTGCATGAACGAGTTCATAANTTGATTGTTGGNTATNAANCTTANAACNANAATTGACAAAGANNTANANTTGCAGAGCNNTAGAGAATCTACTGNANTTGACCAACAAACTGTCTAANNNNGGTGCAAGATAACCAAGACNGNTCACTCCAGNGTTCACTCTGCTNCNNNGGCAGACCCAGNAAGATTTTTAACCAGGGTCAAATCTTAACTAGATANTTTTTTCTGAAAAAAACTTCATAAAAATACTGTAAAACATCCAACAAAAATTCCNTTATAAACATATATAACATCTTATCTAAATCTGGATTNACTATTGTGGAGATGGATAATNTTTTNATCCATGTGGTTAATTAAGAGGAAGATGAAATTTTTGAAACCAAAGAAAGAACATACGTTTCAATGTTTTGTTTAATTGAAATTTTTGCTCTACAAGAATTTGTTANTGGTNNNNNNNNNNNNNTTCATGTTTATTCCGTAAAGAACTATGAGTTCTGAATTATGGAGGTTTAGACATGGTTGTTACGTCGAAACAAAGGTCTCTCTATTTAAATTAAGAAGTTAATGTTGAACTAATTCGAATNATGAATAAAAACATAAAATATTGTATGGNTTTAGNTTATTATTAAAAATAATATGATAGCTGTTTTATAAGGNTAACTTTTAAATTANAAAAACGCACNGTTACNNNNGAACGATGGAATATTAGTTTATTATTCAATAGAGANCGGNACACCAATCTTGCTCCCAACTNTANGNAAACAATCGTTCCATGTCTGTTTGCTTCTTGGAATAGATGCAATATTTTTATATTCATACGATGAGAAGAAGATTTTAATCAATCCAAGATGACAATCTTTGCCAATCTTCAATATATCACGACAACATTTATCTGTCACTANNNTTGTTNCATCCAACACNTTCTGGAAAATCTCACCTCCGCATATNGAGCTCGGCTTTTCAGCGCAAGTNTCAAGAAAATNGAACACTTTTTGCGAGTAATGGGGAAGCTTCTTGTCNTATTCCATTGCGGGGGAATTAGCTTCTTCGAATGGAGTTGGAGATATGGCTACNTCTTCATGTATTTCCTNGGAGAAAATTGGACAANCCATGTAAAACATTACANTGGATACAAGCATGACCATGAGAAGAGACTTTGTCATATTTANTTTTGGTTTTGGATGTAANANTGGTGACATNNNNNNAAAAAAAACAANAGATGTTATATAGGACAATNGGTGNANGTATTTAATTGATAAATTNTTTATTTATTAATANNTTTANNTTANTGANTTCATAAACTTCTTCANTTACTANTTTTGTTNNNNNNNNNNNNNNNNTAGAGACTAGAGCTTNTCCACAAGTTGTAACNTACAAGGTGTGAAAACTCCTTTTGTCTTAATGTTATTACAAATNAAAGTGATACATTAGATATNACACGANTANAAAANAAGATAAAAGAAAGAATTGTAACGAGGANGACCNCATGGAAACTTTCAANCNAAAATACAGCNAGTANTATTTTNGGGAAGTATATGATCNTTAGACTATNATAACAACTAGTGTTTTCNGTAATTTGAATTTTNTNGGGAGAGTAAAGATTCAAGGACGGGGCNGATCATGATACNTNTATCGACCNTTATAACCTCAAANANTTACACACATTCTTATCCTTTAGTNGTTTTAAAACCATACAGGGTTAAGAAAATGGGTGTGATTNAGCTAGCGGTTAGGTTTNCGCTNNNNNNNNNNNNNNNNNNNNNNNNNATGCTTCAGAAATNCATANAGCCGTTNCTTCCGCAANNACGCTACATTTTCCACTTTCTATTTANCAATTAGTCAGCCTTAGAGACCAAGCAACTCACATTCTCTGCAANAAGCCAGCACTCAGAAGGGATGTTGTGGTNTAGATGCTCGATATATGTGGGATCAAACATTGGTGGAATAAATGTCTAATTATTTTCGGAAAACAAATACCAAATATTGGCATTGATTTTGTGATATTNTGAAATATTAATTTACCAACAAAAGATAGATTTTACAGAAATTAACTTAAGCAAATATANAAANTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.523724
PRIMER_LEFT_0_PENALTY=0.026532
PRIMER_RIGHT_0_PENALTY=1.497193
PRIMER_LEFT_0_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_0_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_0=812,20
PRIMER_RIGHT_0=1693,20
PRIMER_LEFT_0_TM=59.973
PRIMER_RIGHT_0_TM=58.503
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=882
PRIMER_PAIR_1_PENALTY=1.557793
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=1.531262
PRIMER_LEFT_1_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_1_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_1=812,20
PRIMER_RIGHT_1=1693,21
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.469
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=882
PRIMER_PAIR_2_PENALTY=1.608270
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=1.497193
PRIMER_LEFT_2_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_2_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_2=813,20
PRIMER_RIGHT_2=1693,20
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=58.503
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=881
PRIMER_PAIR_3_PENALTY=1.642339
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=1.531262
PRIMER_LEFT_3_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_3_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_3=813,20
PRIMER_RIGHT_3=1693,21
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.469
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=881
PRIMER_PAIR_4_PENALTY=2.204519
PRIMER_LEFT_4_PENALTY=0.707326
PRIMER_RIGHT_4_PENALTY=1.497193
PRIMER_LEFT_4_SEQUENCE=TCGTTCCATGTCTGTTTGCT
PRIMER_RIGHT_4_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_4=845,20
PRIMER_RIGHT_4=1693,20
PRIMER_LEFT_4_TM=59.293
PRIMER_RIGHT_4_TM=58.503
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=849
PRIMER_PAIR_5_PENALTY=2.228181
PRIMER_LEFT_5_PENALTY=0.026532
PRIMER_RIGHT_5_PENALTY=2.201650
PRIMER_LEFT_5_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_5_SEQUENCE=TCACACCCATTTTCTTAACCC
PRIMER_LEFT_5=812,20
PRIMER_RIGHT_5=1796,21
PRIMER_LEFT_5_TM=59.973
PRIMER_RIGHT_5_TM=58.798
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=985
PRIMER_PAIR_6_PENALTY=2.238588
PRIMER_LEFT_6_PENALTY=0.707326
PRIMER_RIGHT_6_PENALTY=1.531262
PRIMER_LEFT_6_SEQUENCE=TCGTTCCATGTCTGTTTGCT
PRIMER_RIGHT_6_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_6=845,20
PRIMER_RIGHT_6=1693,21
PRIMER_LEFT_6_TM=59.293
PRIMER_RIGHT_6_TM=59.469
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=849
PRIMER_PAIR_7_PENALTY=2.285440
PRIMER_LEFT_7_PENALTY=0.026532
PRIMER_RIGHT_7_PENALTY=2.258908
PRIMER_LEFT_7_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_7_SEQUENCE=TCACACCCATTTTCTTAACCCT
PRIMER_LEFT_7=812,20
PRIMER_RIGHT_7=1796,22
PRIMER_LEFT_7_TM=59.973
PRIMER_RIGHT_7_TM=59.741
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=9.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=985
PRIMER_PAIR_8_PENALTY=2.312727
PRIMER_LEFT_8_PENALTY=0.111077
PRIMER_RIGHT_8_PENALTY=2.201650
PRIMER_LEFT_8_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_8_SEQUENCE=TCACACCCATTTTCTTAACCC
PRIMER_LEFT_8=813,20
PRIMER_RIGHT_8=1796,21
PRIMER_LEFT_8_TM=60.111
PRIMER_RIGHT_8_TM=58.798
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=984
PRIMER_PAIR_9_PENALTY=2.369985
PRIMER_LEFT_9_PENALTY=0.111077
PRIMER_RIGHT_9_PENALTY=2.258908
PRIMER_LEFT_9_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_9_SEQUENCE=TCACACCCATTTTCTTAACCCT
PRIMER_LEFT_9=813,20
PRIMER_RIGHT_9=1796,22
PRIMER_LEFT_9_TM=60.111
PRIMER_RIGHT_9_TM=59.741
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=9.1000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=984
PRIMER_PAIR_10_PENALTY=2.640570
PRIMER_LEFT_10_PENALTY=0.026532
PRIMER_RIGHT_10_PENALTY=2.614038
PRIMER_LEFT_10_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_10_SEQUENCE=CACACCCATTTTCTTAACCCTG
PRIMER_LEFT_10=812,20
PRIMER_RIGHT_10=1795,22
PRIMER_LEFT_10_TM=59.973
PRIMER_RIGHT_10_TM=60.614
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.455
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=984
PRIMER_PAIR_11_PENALTY=2.722108
PRIMER_LEFT_11_PENALTY=1.224915
PRIMER_RIGHT_11_PENALTY=1.497193
PRIMER_LEFT_11_SEQUENCE=CGTTCCATGTCTGTTTGCTT
PRIMER_RIGHT_11_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_11=846,20
PRIMER_RIGHT_11=1693,20
PRIMER_LEFT_11_TM=58.775
PRIMER_RIGHT_11_TM=58.503
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=848
PRIMER_PAIR_12_PENALTY=2.725115
PRIMER_LEFT_12_PENALTY=0.111077
PRIMER_RIGHT_12_PENALTY=2.614038
PRIMER_LEFT_12_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_12_SEQUENCE=CACACCCATTTTCTTAACCCTG
PRIMER_LEFT_12=813,20
PRIMER_RIGHT_12=1795,22
PRIMER_LEFT_12_TM=60.111
PRIMER_RIGHT_12_TM=60.614
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.455
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=983
PRIMER_PAIR_13_PENALTY=2.756177
PRIMER_LEFT_13_PENALTY=1.224915
PRIMER_RIGHT_13_PENALTY=1.531262
PRIMER_LEFT_13_SEQUENCE=CGTTCCATGTCTGTTTGCTT
PRIMER_RIGHT_13_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_13=846,20
PRIMER_RIGHT_13=1693,21
PRIMER_LEFT_13_TM=58.775
PRIMER_RIGHT_13_TM=59.469
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=5.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=848
PRIMER_PAIR_14_PENALTY=2.885113
PRIMER_LEFT_14_PENALTY=1.387920
PRIMER_RIGHT_14_PENALTY=1.497193
PRIMER_LEFT_14_SEQUENCE=ATCGTTCCATGTCTGTTTGCT
PRIMER_RIGHT_14_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_14=844,21
PRIMER_RIGHT_14=1693,20
PRIMER_LEFT_14_TM=59.612
PRIMER_RIGHT_14_TM=58.503
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=850
PRIMER_PAIR_15_PENALTY=2.908976
PRIMER_LEFT_15_PENALTY=0.707326
PRIMER_RIGHT_15_PENALTY=2.201650
PRIMER_LEFT_15_SEQUENCE=TCGTTCCATGTCTGTTTGCT
PRIMER_RIGHT_15_SEQUENCE=TCACACCCATTTTCTTAACCC
PRIMER_LEFT_15=845,20
PRIMER_RIGHT_15=1796,21
PRIMER_LEFT_15_TM=59.293
PRIMER_RIGHT_15_TM=58.798
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=952
PRIMER_PAIR_16_PENALTY=2.912805
PRIMER_LEFT_16_PENALTY=0.026532
PRIMER_RIGHT_16_PENALTY=2.886273
PRIMER_LEFT_16_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_16_SEQUENCE=ATCACACCCATTTTCTTAACCC
PRIMER_LEFT_16=812,20
PRIMER_RIGHT_16=1797,22
PRIMER_LEFT_16_TM=59.973
PRIMER_RIGHT_16_TM=59.114
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=986
PRIMER_PAIR_17_PENALTY=2.919182
PRIMER_LEFT_17_PENALTY=1.387920
PRIMER_RIGHT_17_PENALTY=1.531262
PRIMER_LEFT_17_SEQUENCE=ATCGTTCCATGTCTGTTTGCT
PRIMER_RIGHT_17_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_17=844,21
PRIMER_RIGHT_17=1693,21
PRIMER_LEFT_17_TM=59.612
PRIMER_RIGHT_17_TM=59.469
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=5.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=850
PRIMER_PAIR_18_PENALTY=2.955740
PRIMER_LEFT_18_PENALTY=0.026532
PRIMER_RIGHT_18_PENALTY=2.929208
PRIMER_LEFT_18_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_18_SEQUENCE=CACCCATTTTCTTAACCCTG
PRIMER_LEFT_18=812,20
PRIMER_RIGHT_18=1793,20
PRIMER_LEFT_18_TM=59.973
PRIMER_RIGHT_18_TM=57.071
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=982
PRIMER_PAIR_19_PENALTY=2.966235
PRIMER_LEFT_19_PENALTY=0.707326
PRIMER_RIGHT_19_PENALTY=2.258908
PRIMER_LEFT_19_SEQUENCE=TCGTTCCATGTCTGTTTGCT
PRIMER_RIGHT_19_SEQUENCE=TCACACCCATTTTCTTAACCCT
PRIMER_LEFT_19=845,20
PRIMER_RIGHT_19=1796,22
PRIMER_LEFT_19_TM=59.293
PRIMER_RIGHT_19_TM=59.741
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=9.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=952
PRIMER_PAIR_20_PENALTY=2.997350
PRIMER_LEFT_20_PENALTY=0.111077
PRIMER_RIGHT_20_PENALTY=2.886273
PRIMER_LEFT_20_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_20_SEQUENCE=ATCACACCCATTTTCTTAACCC
PRIMER_LEFT_20=813,20
PRIMER_RIGHT_20=1797,22
PRIMER_LEFT_20_TM=60.111
PRIMER_RIGHT_20_TM=59.114
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=985
PRIMER_PAIR_21_PENALTY=3.040285
PRIMER_LEFT_21_PENALTY=0.111077
PRIMER_RIGHT_21_PENALTY=2.929208
PRIMER_LEFT_21_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_21_SEQUENCE=CACCCATTTTCTTAACCCTG
PRIMER_LEFT_21=813,20
PRIMER_RIGHT_21=1793,20
PRIMER_LEFT_21_TM=60.111
PRIMER_RIGHT_21_TM=57.071
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=981
PRIMER_PAIR_22_PENALTY=3.046888
PRIMER_LEFT_22_PENALTY=0.026532
PRIMER_RIGHT_22_PENALTY=3.020356
PRIMER_LEFT_22_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_22_SEQUENCE=GCCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_22=812,20
PRIMER_RIGHT_22=1694,21
PRIMER_LEFT_22_TM=59.973
PRIMER_RIGHT_22_TM=62.020
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.0000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=883
PRIMER_PAIR_23_PENALTY=3.056440
PRIMER_LEFT_23_PENALTY=0.026532
PRIMER_RIGHT_23_PENALTY=3.029908
PRIMER_LEFT_23_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_23_SEQUENCE=CACCCATTTTCTTAACCCTGT
PRIMER_LEFT_23=812,20
PRIMER_RIGHT_23=1793,21
PRIMER_LEFT_23_TM=59.973
PRIMER_RIGHT_23_TM=57.970
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=982
PRIMER_PAIR_24_PENALTY=3.056440
PRIMER_LEFT_24_PENALTY=0.026532
PRIMER_RIGHT_24_PENALTY=3.029908
PRIMER_LEFT_24_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_24_SEQUENCE=ACACCCATTTTCTTAACCCTG
PRIMER_LEFT_24=812,20
PRIMER_RIGHT_24=1794,21
PRIMER_LEFT_24_TM=59.973
PRIMER_RIGHT_24_TM=57.970
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=9.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=983
PRIMER_PAIR_25_PENALTY=3.056440
PRIMER_LEFT_25_PENALTY=0.026532
PRIMER_RIGHT_25_PENALTY=3.029908
PRIMER_LEFT_25_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_25_SEQUENCE=CACACCCATTTTCTTAACCCT
PRIMER_LEFT_25=812,20
PRIMER_RIGHT_25=1795,21
PRIMER_LEFT_25_TM=59.973
PRIMER_RIGHT_25_TM=57.970
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=984
PRIMER_PAIR_26_PENALTY=3.112068
PRIMER_LEFT_26_PENALTY=0.026532
PRIMER_RIGHT_26_PENALTY=3.085536
PRIMER_LEFT_26_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_26_SEQUENCE=CACACCCATTTTCTTAACCC
PRIMER_LEFT_26=812,20
PRIMER_RIGHT_26=1795,20
PRIMER_LEFT_26_TM=59.973
PRIMER_RIGHT_26_TM=56.914
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=9.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=984
PRIMER_PAIR_27_PENALTY=3.131433
PRIMER_LEFT_27_PENALTY=0.111077
PRIMER_RIGHT_27_PENALTY=3.020356
PRIMER_LEFT_27_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_27_SEQUENCE=GCCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_27=813,20
PRIMER_RIGHT_27=1694,21
PRIMER_LEFT_27_TM=60.111
PRIMER_RIGHT_27_TM=62.020
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=882
PRIMER_PAIR_28_PENALTY=3.140985
PRIMER_LEFT_28_PENALTY=0.111077
PRIMER_RIGHT_28_PENALTY=3.029908
PRIMER_LEFT_28_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_28_SEQUENCE=CACCCATTTTCTTAACCCTGT
PRIMER_LEFT_28=813,20
PRIMER_RIGHT_28=1793,21
PRIMER_LEFT_28_TM=60.111
PRIMER_RIGHT_28_TM=57.970
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=981
PRIMER_PAIR_29_PENALTY=3.140985
PRIMER_LEFT_29_PENALTY=0.111077
PRIMER_RIGHT_29_PENALTY=3.029908
PRIMER_LEFT_29_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_29_SEQUENCE=ACACCCATTTTCTTAACCCTG
PRIMER_LEFT_29=813,20
PRIMER_RIGHT_29=1794,21
PRIMER_LEFT_29_TM=60.111
PRIMER_RIGHT_29_TM=57.970
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=982
PRIMER_PAIR_30_PENALTY=3.140985
PRIMER_LEFT_30_PENALTY=0.111077
PRIMER_RIGHT_30_PENALTY=3.029908
PRIMER_LEFT_30_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_30_SEQUENCE=CACACCCATTTTCTTAACCCT
PRIMER_LEFT_30=813,20
PRIMER_RIGHT_30=1795,21
PRIMER_LEFT_30_TM=60.111
PRIMER_RIGHT_30_TM=57.970
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=9.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=983
PRIMER_PAIR_31_PENALTY=3.171063
PRIMER_LEFT_31_PENALTY=1.673871
PRIMER_RIGHT_31_PENALTY=1.497193
PRIMER_LEFT_31_SEQUENCE=CCATGTCTGTTTGCTTCTTGG
PRIMER_RIGHT_31_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_31=850,21
PRIMER_RIGHT_31=1693,20
PRIMER_LEFT_31_TM=60.674
PRIMER_RIGHT_31_TM=58.503
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=6.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=844
PRIMER_PAIR_32_PENALTY=3.180787
PRIMER_LEFT_32_PENALTY=1.683594
PRIMER_RIGHT_32_PENALTY=1.497193
PRIMER_LEFT_32_SEQUENCE=CGTTCCATGTCTGTTTGCTTC
PRIMER_RIGHT_32_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_32=846,21
PRIMER_RIGHT_32=1693,20
PRIMER_LEFT_32_TM=60.684
PRIMER_RIGHT_32_TM=58.503
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=848
PRIMER_PAIR_33_PENALTY=3.180787
PRIMER_LEFT_33_PENALTY=1.683594
PRIMER_RIGHT_33_PENALTY=1.497193
PRIMER_LEFT_33_SEQUENCE=TCGTTCCATGTCTGTTTGCTT
PRIMER_RIGHT_33_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_33=845,21
PRIMER_RIGHT_33=1693,20
PRIMER_LEFT_33_TM=60.684
PRIMER_RIGHT_33_TM=58.503
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=849
PRIMER_PAIR_34_PENALTY=3.193043
PRIMER_LEFT_34_PENALTY=0.026532
PRIMER_RIGHT_34_PENALTY=3.166511
PRIMER_LEFT_34_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_34_SEQUENCE=CCCCGTCCTTGAATCTTTACTC
PRIMER_LEFT_34=812,20
PRIMER_RIGHT_34=1693,22
PRIMER_LEFT_34_TM=59.973
PRIMER_RIGHT_34_TM=61.167
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=5.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=882
PRIMER_PAIR_35_PENALTY=3.196613
PRIMER_LEFT_35_PENALTY=0.111077
PRIMER_RIGHT_35_PENALTY=3.085536
PRIMER_LEFT_35_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_35_SEQUENCE=CACACCCATTTTCTTAACCC
PRIMER_LEFT_35=813,20
PRIMER_RIGHT_35=1795,20
PRIMER_LEFT_35_TM=60.111
PRIMER_RIGHT_35_TM=56.914
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=983
PRIMER_PAIR_36_PENALTY=3.205133
PRIMER_LEFT_36_PENALTY=1.673871
PRIMER_RIGHT_36_PENALTY=1.531262
PRIMER_LEFT_36_SEQUENCE=CCATGTCTGTTTGCTTCTTGG
PRIMER_RIGHT_36_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_36=850,21
PRIMER_RIGHT_36=1693,21
PRIMER_LEFT_36_TM=60.674
PRIMER_RIGHT_36_TM=59.469
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=5.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=844
PRIMER_PAIR_37_PENALTY=3.214856
PRIMER_LEFT_37_PENALTY=1.683594
PRIMER_RIGHT_37_PENALTY=1.531262
PRIMER_LEFT_37_SEQUENCE=CGTTCCATGTCTGTTTGCTTC
PRIMER_RIGHT_37_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_37=846,21
PRIMER_RIGHT_37=1693,21
PRIMER_LEFT_37_TM=60.684
PRIMER_RIGHT_37_TM=59.469
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=5.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=848
PRIMER_PAIR_38_PENALTY=3.214856
PRIMER_LEFT_38_PENALTY=1.683594
PRIMER_RIGHT_38_PENALTY=1.531262
PRIMER_LEFT_38_SEQUENCE=TCGTTCCATGTCTGTTTGCTT
PRIMER_RIGHT_38_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_38=845,21
PRIMER_RIGHT_38=1693,21
PRIMER_LEFT_38_TM=60.684
PRIMER_RIGHT_38_TM=59.469
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=5.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=849
PRIMER_PAIR_39_PENALTY=3.222351
PRIMER_LEFT_39_PENALTY=0.026532
PRIMER_RIGHT_39_PENALTY=3.195819
PRIMER_LEFT_39_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_39_SEQUENCE=ACACCCATTTTCTTAACCCTGT
PRIMER_LEFT_39=812,20
PRIMER_RIGHT_39=1794,22
PRIMER_LEFT_39_TM=59.973
PRIMER_RIGHT_39_TM=58.804
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=983
PRIMER_PAIR_40_PENALTY=3.226675
PRIMER_LEFT_40_PENALTY=0.026532
PRIMER_RIGHT_40_PENALTY=3.200143
PRIMER_LEFT_40_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_40_SEQUENCE=CCCGTCCTTGAATCTTTACTC
PRIMER_LEFT_40=812,20
PRIMER_RIGHT_40=1692,21
PRIMER_LEFT_40_TM=59.973
PRIMER_RIGHT_40_TM=57.800
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=5.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=881
PRIMER_PAIR_41_PENALTY=3.245654
PRIMER_LEFT_41_PENALTY=0.026532
PRIMER_RIGHT_41_PENALTY=3.219122
PRIMER_LEFT_41_SEQUENCE=ACACCAATCTTGCTCCCAAC
PRIMER_RIGHT_41_SEQUENCE=CCCGTCCTTGAATCTTTACTCT
PRIMER_LEFT_41=812,20
PRIMER_RIGHT_41=1692,22
PRIMER_LEFT_41_TM=59.973
PRIMER_RIGHT_41_TM=58.781
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.1000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=881
PRIMER_PAIR_42_PENALTY=3.277588
PRIMER_LEFT_42_PENALTY=0.111077
PRIMER_RIGHT_42_PENALTY=3.166511
PRIMER_LEFT_42_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_42_SEQUENCE=CCCCGTCCTTGAATCTTTACTC
PRIMER_LEFT_42=813,20
PRIMER_RIGHT_42=1693,22
PRIMER_LEFT_42_TM=60.111
PRIMER_RIGHT_42_TM=61.167
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=5.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=881
PRIMER_PAIR_43_PENALTY=3.306896
PRIMER_LEFT_43_PENALTY=0.111077
PRIMER_RIGHT_43_PENALTY=3.195819
PRIMER_LEFT_43_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_43_SEQUENCE=ACACCCATTTTCTTAACCCTGT
PRIMER_LEFT_43=813,20
PRIMER_RIGHT_43=1794,22
PRIMER_LEFT_43_TM=60.111
PRIMER_RIGHT_43_TM=58.804
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=982
PRIMER_PAIR_44_PENALTY=3.311220
PRIMER_LEFT_44_PENALTY=0.111077
PRIMER_RIGHT_44_PENALTY=3.200143
PRIMER_LEFT_44_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_44_SEQUENCE=CCCGTCCTTGAATCTTTACTC
PRIMER_LEFT_44=813,20
PRIMER_RIGHT_44=1692,21
PRIMER_LEFT_44_TM=60.111
PRIMER_RIGHT_44_TM=57.800
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=5.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=880
PRIMER_PAIR_45_PENALTY=3.321365
PRIMER_LEFT_45_PENALTY=0.707326
PRIMER_RIGHT_45_PENALTY=2.614038
PRIMER_LEFT_45_SEQUENCE=TCGTTCCATGTCTGTTTGCT
PRIMER_RIGHT_45_SEQUENCE=CACACCCATTTTCTTAACCCTG
PRIMER_LEFT_45=845,20
PRIMER_RIGHT_45=1795,22
PRIMER_LEFT_45_TM=59.293
PRIMER_RIGHT_45_TM=60.614
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=951
PRIMER_PAIR_46_PENALTY=3.330199
PRIMER_LEFT_46_PENALTY=0.111077
PRIMER_RIGHT_46_PENALTY=3.219122
PRIMER_LEFT_46_SEQUENCE=CACCAATCTTGCTCCCAACT
PRIMER_RIGHT_46_SEQUENCE=CCCGTCCTTGAATCTTTACTCT
PRIMER_LEFT_46=813,20
PRIMER_RIGHT_46=1692,22
PRIMER_LEFT_46_TM=60.111
PRIMER_RIGHT_46_TM=58.781
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.1000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=880
PRIMER_PAIR_47_PENALTY=3.424429
PRIMER_LEFT_47_PENALTY=1.927237
PRIMER_RIGHT_47_PENALTY=1.497193
PRIMER_LEFT_47_SEQUENCE=ACACCAATCTTGCTCCCAACT
PRIMER_RIGHT_47_SEQUENCE=CCCCGTCCTTGAATCTTTAC
PRIMER_LEFT_47=812,21
PRIMER_RIGHT_47=1693,20
PRIMER_LEFT_47_TM=60.927
PRIMER_RIGHT_47_TM=58.503
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=882
PRIMER_PAIR_48_PENALTY=3.426565
PRIMER_LEFT_48_PENALTY=1.224915
PRIMER_RIGHT_48_PENALTY=2.201650
PRIMER_LEFT_48_SEQUENCE=CGTTCCATGTCTGTTTGCTT
PRIMER_RIGHT_48_SEQUENCE=TCACACCCATTTTCTTAACCC
PRIMER_LEFT_48=846,20
PRIMER_RIGHT_48=1796,21
PRIMER_LEFT_48_TM=58.775
PRIMER_RIGHT_48_TM=58.798
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=951
PRIMER_PAIR_49_PENALTY=3.458498
PRIMER_LEFT_49_PENALTY=1.927237
PRIMER_RIGHT_49_PENALTY=1.531262
PRIMER_LEFT_49_SEQUENCE=ACACCAATCTTGCTCCCAACT
PRIMER_RIGHT_49_SEQUENCE=CCCCGTCCTTGAATCTTTACT
PRIMER_LEFT_49=812,21
PRIMER_RIGHT_49=1693,21
PRIMER_LEFT_49_TM=60.927
PRIMER_RIGHT_49_TM=59.469
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=5.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=882
=
SEQUENCE_ID=MAGIC.105_4_7648740_7649199_po-0_edi-0_20614_117333_TRUE
SEQUENCE_TARGET=950,559
SEQUENCE_TEMPLATE=NCGGGTTCNNAGCTCGTTTNCCTCATTACACAGTTGATGCGTANCGATACCTCTGTGGATTCGGATTCAGAGCCAACACAAGAATCTGAGCTTCTATCACTCATTAGCCAACTAGTNTCTNNCAAGCCGGAGCCGGAGCTCACATCACTCACCAGNCGGATCATCTCTACCTTCATATCTATGAATTGGGAGCCNATGCAGTTTATTTCCGTATGCCCTCAANTTANGGTNNAACTTGTANATGNAAAANTGACGACAGAAGTTGNCNGGNCGAGAAANGCTAAAGAGCGGGAATGTGAGTTGTATNCTTACNAANTTTGGAAATCTACAAGACAAATCCATTTTNACTGTANACNATGCAATGGCGAGAACCATGANGANTATGACAAGGTTCCTATAGAGGTCNNNNNNNNNCTTCACCCAAGACATTCTCTTCAGCTTGCCGTTNGACAATGGTCNNNGGAGGAAACGATGATNGAATGTTATTGTTGTGATGAATATCTCATGGATTATTATTACTATTGCTCCTCTTGCGATTTTGCTATGAATGTAAGCTGTTTGGAGAAACCACCACCAGTCTTATCTATAGACCATCCCAAGTGGCATCAGCATCCCCTTATTTTGTTTCCAAGTCAGGCTTCCTTTCNTTGTAACCTTTGTGCCNTCACACATNCAAGTTGTCCTTTCTATATNTGTCCCCCNTGTGACTTNGTGGTCCATCAAAAGTGTCTCAGCTTACCACATGTCATAAGGATATCCCGCCACCNNCNTCGTATCTCCTTCACTCCNTCATTTGAGAAAGGAAATTNGTATTGTGGTATTTGTCGNAAAAAGATGGANAATGATTACGGTGGTTATNCTTGCCTCAAGGANGGTTGTTCNTATGTGGCACATTCAAGATGTGCAACACAAAAGAATGTGTGGGATGGTCTAGACCTTGAGGGAGANCCNNNNNNNNNNNNNNNAGAAGAAGTTGAGCCATTTGTGATAATAAGNGANGNAACAATACACCATTTTAGTCATCCACATCATTTGAGAATNGATAANAATACCNGCAGAGACTACGACGAGAATANGCAGTGTCAAGNATGCATCACACCAATCTATTTTGGTAACATCTACTCNTGTATGNAATGCAACTTCATTCTCCATGAAGAATGTGCNAATTTNTCTCGCAAAATACATAACCCGATACATCCACATAANCTCANTCTAGTNGGAGGANACGANGNTGTTNCCAAGTATNANAANGNTTTCTGTTCAGNNTGNCCTAGGATGTGCANNAATGGTTTCTTNTATGAGTGCGGTAAAGAAGAATGTGATCGTTTTAAGCTACATGTGCAGTGTGCCACAATTTCTGAGCCATTAGTCCATGAAAGTCATGTGCATCCTTTATTCTTAACATCNAAACCANGAGAACNAAGAAGATGTGGGGTTTGCAAANANANGCCACTTNGCCNCNCGGAAACATTCAATTGTATTGAAGGTGANTGTANCTTTGCTTTGTGTTTTGGATGTGCTACTTTACCTCACGAGGTGAGGTATAAGCATGATAAGCACATGCTCACTCTTTCTTATGGGGAGGAGACAAGTACCATGATGTNTTGGTGTGAAGCCTGCGANAAAGAGATAAATNCAGAAGAACGGTTNTATAAGTGTGATGAATANTGTTGTGTCACCCTGCACATTCAATGTTTGATTGGGAANGAATTATACTTGAAGCCNGGTTCNTCGTTTGAGTACNACNANAGGCATGCAGATGTTCTTTTAAAAACTCAGGTTATGTCTCGACCTATTTGCTTCNANTGTNAGAGTCGTTGTCCACATAACACAGTTATCCAGTGGTCTGAGTTAGTATNTTGCANCATNNANTGTTTTTTGNTTGNNNGACNAAGANGGGACGNAGAAGAAAGAAGAAGAAANNAAGCTNNNNTTGANAATAAAGATGTTTGAATGAAATCCCAATTTAGGAGACTANNNNTTTGAGTANAGTNATCAAAGAGANTCTGNTGTNCATTGTANGTTTTGAATCTTNGAANNNNNNNNNNNNNNNNNNNNNNGTTNATGTGTGATTGTGAACATNGTCTTGTNTAATTGAANCTTTGATTTGTGAAGGAGACNGCTAGACGAACTAGNACGNNACATTGGTAGGAAACCANTATAATACCACTNTTGTNACAACAAAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTTGTNTATATTTCCAANTTTGATATGNTGTCACATGNNNNNNNNTTAGTATCATCACANATCTGANGAGTCTGCTANAATGGAGTTCGAATTTTTGTAAATACNCTTAAGATGTCCCAATCCNNAANACAAAACAGTTGTTTTNNAAAACTCNNNNGAGCAAGTTTGNNTNTAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.733127
PRIMER_LEFT_0_PENALTY=0.232299
PRIMER_RIGHT_0_PENALTY=0.500828
PRIMER_LEFT_0_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_0_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_0=622,20
PRIMER_RIGHT_0=1596,20
PRIMER_LEFT_0_TM=60.232
PRIMER_RIGHT_0_TM=60.501
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=975
PRIMER_PAIR_1_PENALTY=1.095541
PRIMER_LEFT_1_PENALTY=0.594713
PRIMER_RIGHT_1_PENALTY=0.500828
PRIMER_LEFT_1_SEQUENCE=TCCAAGTCAGGCTTCCTTTC
PRIMER_RIGHT_1_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_1=626,20
PRIMER_RIGHT_1=1596,20
PRIMER_LEFT_1_TM=59.405
PRIMER_RIGHT_1_TM=60.501
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=971
PRIMER_PAIR_2_PENALTY=1.499481
PRIMER_LEFT_2_PENALTY=0.998653
PRIMER_RIGHT_2_PENALTY=0.500828
PRIMER_LEFT_2_SEQUENCE=GCATCAGCATCCCCTTATTT
PRIMER_RIGHT_2_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_2=602,20
PRIMER_RIGHT_2=1596,20
PRIMER_LEFT_2_TM=59.001
PRIMER_RIGHT_2_TM=60.501
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=995
PRIMER_PAIR_3_PENALTY=1.677335
PRIMER_LEFT_3_PENALTY=1.176507
PRIMER_RIGHT_3_PENALTY=0.500828
PRIMER_LEFT_3_SEQUENCE=GGCATCAGCATCCCCTTATT
PRIMER_RIGHT_3_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_3=601,20
PRIMER_RIGHT_3=1596,20
PRIMER_LEFT_3_TM=61.177
PRIMER_RIGHT_3_TM=60.501
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=996
PRIMER_PAIR_4_PENALTY=1.732931
PRIMER_LEFT_4_PENALTY=1.179024
PRIMER_RIGHT_4_PENALTY=0.553907
PRIMER_LEFT_4_SEQUENCE=CAAAAGAATGTGTGGGATGG
PRIMER_RIGHT_4_SEQUENCE=AATGTGCAGGGTGACACAAC
PRIMER_LEFT_4=909,20
PRIMER_RIGHT_4=1688,20
PRIMER_LEFT_4_TM=58.821
PRIMER_RIGHT_4_TM=59.446
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=780
PRIMER_PAIR_5_PENALTY=1.794467
PRIMER_LEFT_5_PENALTY=1.293639
PRIMER_RIGHT_5_PENALTY=0.500828
PRIMER_LEFT_5_SEQUENCE=GCATCAGCATCCCCTTATTTT
PRIMER_RIGHT_5_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_5=602,21
PRIMER_RIGHT_5=1596,20
PRIMER_LEFT_5_TM=60.294
PRIMER_RIGHT_5_TM=60.501
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=11.2000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=995
PRIMER_PAIR_6_PENALTY=1.798277
PRIMER_LEFT_6_PENALTY=1.297449
PRIMER_RIGHT_6_PENALTY=0.500828
PRIMER_LEFT_6_SEQUENCE=CAAAAGAATGTGTGGGATGGT
PRIMER_RIGHT_6_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_6=909,21
PRIMER_RIGHT_6=1596,20
PRIMER_LEFT_6_TM=59.703
PRIMER_RIGHT_6_TM=60.501
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.8000
PRIMER_RIGHT_6_END_STABILITY=11.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=688
PRIMER_PAIR_7_PENALTY=1.829110
PRIMER_LEFT_7_PENALTY=1.328282
PRIMER_RIGHT_7_PENALTY=0.500828
PRIMER_LEFT_7_SEQUENCE=GCATCCCCTTATTTTGTTTCC
PRIMER_RIGHT_7_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_7=608,21
PRIMER_RIGHT_7=1596,20
PRIMER_LEFT_7_TM=59.672
PRIMER_RIGHT_7_TM=60.501
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=11.2000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=989
PRIMER_PAIR_8_PENALTY=1.851356
PRIMER_LEFT_8_PENALTY=1.297449
PRIMER_RIGHT_8_PENALTY=0.553907
PRIMER_LEFT_8_SEQUENCE=CAAAAGAATGTGTGGGATGGT
PRIMER_RIGHT_8_SEQUENCE=AATGTGCAGGGTGACACAAC
PRIMER_LEFT_8=909,21
PRIMER_RIGHT_8=1688,20
PRIMER_LEFT_8_TM=59.703
PRIMER_RIGHT_8_TM=59.446
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.8000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=780
PRIMER_PAIR_9_PENALTY=1.851356
PRIMER_LEFT_9_PENALTY=1.297449
PRIMER_RIGHT_9_PENALTY=0.553907
PRIMER_LEFT_9_SEQUENCE=ACAAAAGAATGTGTGGGATGG
PRIMER_RIGHT_9_SEQUENCE=AATGTGCAGGGTGACACAAC
PRIMER_LEFT_9=908,21
PRIMER_RIGHT_9=1688,20
PRIMER_LEFT_9_TM=59.703
PRIMER_RIGHT_9_TM=59.446
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=781
PRIMER_PAIR_10_PENALTY=1.983150
PRIMER_LEFT_10_PENALTY=0.232299
PRIMER_RIGHT_10_PENALTY=1.750851
PRIMER_LEFT_10_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_10_SEQUENCE=ATGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_10=622,20
PRIMER_RIGHT_10=1597,21
PRIMER_LEFT_10_TM=60.232
PRIMER_RIGHT_10_TM=60.751
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=11.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=976
PRIMER_PAIR_11_PENALTY=1.983150
PRIMER_LEFT_11_PENALTY=0.232299
PRIMER_RIGHT_11_PENALTY=1.750851
PRIMER_LEFT_11_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_11_SEQUENCE=CATGGTACTTGTCTCCTCCCC
PRIMER_LEFT_11=622,20
PRIMER_RIGHT_11=1598,21
PRIMER_LEFT_11_TM=60.232
PRIMER_RIGHT_11_TM=60.751
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=57.143
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=10.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=977
PRIMER_PAIR_12_PENALTY=2.081014
PRIMER_LEFT_12_PENALTY=1.580186
PRIMER_RIGHT_12_PENALTY=0.500828
PRIMER_LEFT_12_SEQUENCE=CATCAGCATCCCCTTATTTTG
PRIMER_RIGHT_12_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_12=603,21
PRIMER_RIGHT_12=1596,20
PRIMER_LEFT_12_TM=59.420
PRIMER_RIGHT_12_TM=60.501
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=11.2000
PRIMER_PAIR_12_COMPL_ANY=2.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=994
PRIMER_PAIR_13_PENALTY=2.228978
PRIMER_LEFT_13_PENALTY=0.232299
PRIMER_RIGHT_13_PENALTY=1.996678
PRIMER_LEFT_13_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_13_SEQUENCE=TCATGGTACTTGTCTCCTCCC
PRIMER_LEFT_13=622,20
PRIMER_RIGHT_13=1599,21
PRIMER_LEFT_13_TM=60.232
PRIMER_RIGHT_13_TM=59.003
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=978
PRIMER_PAIR_14_PENALTY=2.229160
PRIMER_LEFT_14_PENALTY=1.728332
PRIMER_RIGHT_14_PENALTY=0.500828
PRIMER_LEFT_14_SEQUENCE=TTCCAAGTCAGGCTTCCTTTC
PRIMER_RIGHT_14_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_14=625,21
PRIMER_RIGHT_14=1596,20
PRIMER_LEFT_14_TM=60.728
PRIMER_RIGHT_14_TM=60.501
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=11.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=972
PRIMER_PAIR_15_PENALTY=2.335691
PRIMER_LEFT_15_PENALTY=0.232299
PRIMER_RIGHT_15_PENALTY=2.103392
PRIMER_LEFT_15_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_15_SEQUENCE=ATGGTACTTGTCTCCTCCCC
PRIMER_LEFT_15=622,20
PRIMER_RIGHT_15=1597,20
PRIMER_LEFT_15_TM=60.232
PRIMER_RIGHT_15_TM=57.897
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=10.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=976
PRIMER_PAIR_16_PENALTY=2.345564
PRIMER_LEFT_16_PENALTY=0.594713
PRIMER_RIGHT_16_PENALTY=1.750851
PRIMER_LEFT_16_SEQUENCE=TCCAAGTCAGGCTTCCTTTC
PRIMER_RIGHT_16_SEQUENCE=ATGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_16=626,20
PRIMER_RIGHT_16=1597,21
PRIMER_LEFT_16_TM=59.405
PRIMER_RIGHT_16_TM=60.751
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=11.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=972
PRIMER_PAIR_17_PENALTY=2.345564
PRIMER_LEFT_17_PENALTY=0.594713
PRIMER_RIGHT_17_PENALTY=1.750851
PRIMER_LEFT_17_SEQUENCE=TCCAAGTCAGGCTTCCTTTC
PRIMER_RIGHT_17_SEQUENCE=CATGGTACTTGTCTCCTCCCC
PRIMER_LEFT_17=626,20
PRIMER_RIGHT_17=1598,21
PRIMER_LEFT_17_TM=59.405
PRIMER_RIGHT_17_TM=60.751
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=57.143
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=10.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=973
PRIMER_PAIR_18_PENALTY=2.377102
PRIMER_LEFT_18_PENALTY=1.876274
PRIMER_RIGHT_18_PENALTY=0.500828
PRIMER_LEFT_18_SEQUENCE=TGGTCCATCAAAAGTGTCTCA
PRIMER_RIGHT_18_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_18=712,21
PRIMER_RIGHT_18=1596,20
PRIMER_LEFT_18_TM=59.124
PRIMER_RIGHT_18_TM=60.501
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=11.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=885
PRIMER_PAIR_19_PENALTY=2.513298
PRIMER_LEFT_19_PENALTY=2.012470
PRIMER_RIGHT_19_PENALTY=0.500828
PRIMER_LEFT_19_SEQUENCE=GTGGTCCATCAAAAGTGTCTCA
PRIMER_RIGHT_19_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_19=711,22
PRIMER_RIGHT_19=1596,20
PRIMER_LEFT_19_TM=60.012
PRIMER_RIGHT_19_TM=60.501
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=11.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=886
PRIMER_PAIR_20_PENALTY=2.591391
PRIMER_LEFT_20_PENALTY=0.594713
PRIMER_RIGHT_20_PENALTY=1.996678
PRIMER_LEFT_20_SEQUENCE=TCCAAGTCAGGCTTCCTTTC
PRIMER_RIGHT_20_SEQUENCE=TCATGGTACTTGTCTCCTCCC
PRIMER_LEFT_20=626,20
PRIMER_RIGHT_20=1599,21
PRIMER_LEFT_20_TM=59.405
PRIMER_RIGHT_20_TM=59.003
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=974
PRIMER_PAIR_21_PENALTY=2.600115
PRIMER_LEFT_21_PENALTY=0.232299
PRIMER_RIGHT_21_PENALTY=2.367816
PRIMER_LEFT_21_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_21_SEQUENCE=TTGTCTCCTCCCCATAAGAAA
PRIMER_LEFT_21=622,20
PRIMER_RIGHT_21=1590,21
PRIMER_LEFT_21_TM=60.232
PRIMER_RIGHT_21_TM=58.632
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=969
PRIMER_PAIR_22_PENALTY=2.644216
PRIMER_LEFT_22_PENALTY=0.232299
PRIMER_RIGHT_22_PENALTY=2.411917
PRIMER_LEFT_22_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_22_SEQUENCE=TTGTCTCCTCCCCATAAGAAAG
PRIMER_LEFT_22=622,20
PRIMER_RIGHT_22=1590,22
PRIMER_LEFT_22_TM=60.232
PRIMER_RIGHT_22_TM=59.588
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=969
PRIMER_PAIR_23_PENALTY=2.644216
PRIMER_LEFT_23_PENALTY=0.232299
PRIMER_RIGHT_23_PENALTY=2.411917
PRIMER_LEFT_23_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_23_SEQUENCE=CTTGTCTCCTCCCCATAAGAAA
PRIMER_LEFT_23=622,20
PRIMER_RIGHT_23=1591,22
PRIMER_LEFT_23_TM=60.232
PRIMER_RIGHT_23_TM=59.588
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=970
PRIMER_PAIR_24_PENALTY=2.657307
PRIMER_LEFT_24_PENALTY=2.156479
PRIMER_RIGHT_24_PENALTY=0.500828
PRIMER_LEFT_24_SEQUENCE=CAGCATCCCCTTATTTTGTTTC
PRIMER_RIGHT_24_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_24=606,22
PRIMER_RIGHT_24=1596,20
PRIMER_LEFT_24_TM=59.844
PRIMER_RIGHT_24_TM=60.501
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=11.2000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=991
PRIMER_PAIR_25_PENALTY=2.668439
PRIMER_LEFT_25_PENALTY=2.114532
PRIMER_RIGHT_25_PENALTY=0.553907
PRIMER_LEFT_25_SEQUENCE=CACAAAAGAATGTGTGGGATG
PRIMER_RIGHT_25_SEQUENCE=AATGTGCAGGGTGACACAAC
PRIMER_LEFT_25=907,21
PRIMER_RIGHT_25=1688,20
PRIMER_LEFT_25_TM=58.885
PRIMER_RIGHT_25_TM=59.446
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=782
PRIMER_PAIR_26_PENALTY=2.698105
PRIMER_LEFT_26_PENALTY=0.594713
PRIMER_RIGHT_26_PENALTY=2.103392
PRIMER_LEFT_26_SEQUENCE=TCCAAGTCAGGCTTCCTTTC
PRIMER_RIGHT_26_SEQUENCE=ATGGTACTTGTCTCCTCCCC
PRIMER_LEFT_26=626,20
PRIMER_RIGHT_26=1597,20
PRIMER_LEFT_26_TM=59.405
PRIMER_RIGHT_26_TM=57.897
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=10.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=972
PRIMER_PAIR_27_PENALTY=2.704799
PRIMER_LEFT_27_PENALTY=2.203971
PRIMER_RIGHT_27_PENALTY=0.500828
PRIMER_LEFT_27_SEQUENCE=CATCAGCATCCCCTTATTTTGT
PRIMER_RIGHT_27_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_27=603,22
PRIMER_RIGHT_27=1596,20
PRIMER_LEFT_27_TM=60.204
PRIMER_RIGHT_27_TM=60.501
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=11.2000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=994
PRIMER_PAIR_28_PENALTY=2.746162
PRIMER_LEFT_28_PENALTY=2.245334
PRIMER_RIGHT_28_PENALTY=0.500828
PRIMER_LEFT_28_SEQUENCE=AAAAGAATGTGTGGGATGGTC
PRIMER_RIGHT_28_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_28=910,21
PRIMER_RIGHT_28=1596,20
PRIMER_LEFT_28_TM=58.755
PRIMER_RIGHT_28_TM=60.501
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=11.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=687
PRIMER_PAIR_29_PENALTY=2.749503
PRIMER_LEFT_29_PENALTY=0.998653
PRIMER_RIGHT_29_PENALTY=1.750851
PRIMER_LEFT_29_SEQUENCE=GCATCAGCATCCCCTTATTT
PRIMER_RIGHT_29_SEQUENCE=ATGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_29=602,20
PRIMER_RIGHT_29=1597,21
PRIMER_LEFT_29_TM=59.001
PRIMER_RIGHT_29_TM=60.751
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.2000
PRIMER_RIGHT_29_END_STABILITY=11.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=996
PRIMER_PAIR_30_PENALTY=2.749503
PRIMER_LEFT_30_PENALTY=0.998653
PRIMER_RIGHT_30_PENALTY=1.750851
PRIMER_LEFT_30_SEQUENCE=GCATCAGCATCCCCTTATTT
PRIMER_RIGHT_30_SEQUENCE=CATGGTACTTGTCTCCTCCCC
PRIMER_LEFT_30=602,20
PRIMER_RIGHT_30=1598,21
PRIMER_LEFT_30_TM=59.001
PRIMER_RIGHT_30_TM=60.751
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=57.143
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.2000
PRIMER_RIGHT_30_END_STABILITY=10.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=997
PRIMER_PAIR_31_PENALTY=2.765881
PRIMER_LEFT_31_PENALTY=2.265053
PRIMER_RIGHT_31_PENALTY=0.500828
PRIMER_LEFT_31_SEQUENCE=AAAAGAATGTGTGGGATGGTCT
PRIMER_RIGHT_31_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_31=910,22
PRIMER_RIGHT_31=1596,20
PRIMER_LEFT_31_TM=59.735
PRIMER_RIGHT_31_TM=60.501
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=11.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=687
PRIMER_PAIR_32_PENALTY=2.796649
PRIMER_LEFT_32_PENALTY=2.242742
PRIMER_RIGHT_32_PENALTY=0.553907
PRIMER_LEFT_32_SEQUENCE=ACACAAAAGAATGTGTGGGATG
PRIMER_RIGHT_32_SEQUENCE=AATGTGCAGGGTGACACAAC
PRIMER_LEFT_32=906,22
PRIMER_RIGHT_32=1688,20
PRIMER_LEFT_32_TM=59.757
PRIMER_RIGHT_32_TM=59.446
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=783
PRIMER_PAIR_33_PENALTY=2.799241
PRIMER_LEFT_33_PENALTY=2.245334
PRIMER_RIGHT_33_PENALTY=0.553907
PRIMER_LEFT_33_SEQUENCE=AAAAGAATGTGTGGGATGGTC
PRIMER_RIGHT_33_SEQUENCE=AATGTGCAGGGTGACACAAC
PRIMER_LEFT_33=910,21
PRIMER_RIGHT_33=1688,20
PRIMER_LEFT_33_TM=58.755
PRIMER_RIGHT_33_TM=59.446
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=779
PRIMER_PAIR_34_PENALTY=2.818960
PRIMER_LEFT_34_PENALTY=2.265053
PRIMER_RIGHT_34_PENALTY=0.553907
PRIMER_LEFT_34_SEQUENCE=AAAAGAATGTGTGGGATGGTCT
PRIMER_RIGHT_34_SEQUENCE=AATGTGCAGGGTGACACAAC
PRIMER_LEFT_34=910,22
PRIMER_RIGHT_34=1688,20
PRIMER_LEFT_34_TM=59.735
PRIMER_RIGHT_34_TM=59.446
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.6000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=779
PRIMER_PAIR_35_PENALTY=2.883981
PRIMER_LEFT_35_PENALTY=0.232299
PRIMER_RIGHT_35_PENALTY=2.651681
PRIMER_LEFT_35_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_35_SEQUENCE=GGTACTTGTCTCCTCCCCATAA
PRIMER_LEFT_35=622,20
PRIMER_RIGHT_35=1595,22
PRIMER_LEFT_35_TM=60.232
PRIMER_RIGHT_35_TM=59.348
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=974
PRIMER_PAIR_36_PENALTY=2.905267
PRIMER_LEFT_36_PENALTY=0.232299
PRIMER_RIGHT_36_PENALTY=2.672968
PRIMER_LEFT_36_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_36_SEQUENCE=ATCATGGTACTTGTCTCCTCCC
PRIMER_LEFT_36=622,20
PRIMER_RIGHT_36=1600,22
PRIMER_LEFT_36_TM=60.232
PRIMER_RIGHT_36_TM=59.327
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=979
PRIMER_PAIR_37_PENALTY=2.927357
PRIMER_LEFT_37_PENALTY=1.176507
PRIMER_RIGHT_37_PENALTY=1.750851
PRIMER_LEFT_37_SEQUENCE=GGCATCAGCATCCCCTTATT
PRIMER_RIGHT_37_SEQUENCE=ATGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_37=601,20
PRIMER_RIGHT_37=1597,21
PRIMER_LEFT_37_TM=61.177
PRIMER_RIGHT_37_TM=60.751
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.2000
PRIMER_RIGHT_37_END_STABILITY=11.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=997
PRIMER_PAIR_38_PENALTY=2.927357
PRIMER_LEFT_38_PENALTY=1.176507
PRIMER_RIGHT_38_PENALTY=1.750851
PRIMER_LEFT_38_SEQUENCE=GGCATCAGCATCCCCTTATT
PRIMER_RIGHT_38_SEQUENCE=CATGGTACTTGTCTCCTCCCC
PRIMER_LEFT_38=601,20
PRIMER_RIGHT_38=1598,21
PRIMER_LEFT_38_TM=61.177
PRIMER_RIGHT_38_TM=60.751
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=57.143
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.2000
PRIMER_RIGHT_38_END_STABILITY=10.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=998
PRIMER_PAIR_39_PENALTY=2.929875
PRIMER_LEFT_39_PENALTY=1.179024
PRIMER_RIGHT_39_PENALTY=1.750851
PRIMER_LEFT_39_SEQUENCE=CAAAAGAATGTGTGGGATGG
PRIMER_RIGHT_39_SEQUENCE=CATGGTACTTGTCTCCTCCCC
PRIMER_LEFT_39=909,20
PRIMER_RIGHT_39=1598,21
PRIMER_LEFT_39_TM=58.821
PRIMER_RIGHT_39_TM=60.751
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=57.143
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.1000
PRIMER_RIGHT_39_END_STABILITY=10.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=690
PRIMER_PAIR_40_PENALTY=2.979067
PRIMER_LEFT_40_PENALTY=0.232299
PRIMER_RIGHT_40_PENALTY=2.746768
PRIMER_LEFT_40_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_40_SEQUENCE=TCCTCCCCATAAGAAAGAGTG
PRIMER_LEFT_40=622,20
PRIMER_RIGHT_40=1585,21
PRIMER_LEFT_40_TM=60.232
PRIMER_RIGHT_40_TM=58.253
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=964
PRIMER_PAIR_41_PENALTY=2.979067
PRIMER_LEFT_41_PENALTY=0.232299
PRIMER_RIGHT_41_PENALTY=2.746768
PRIMER_LEFT_41_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_41_SEQUENCE=TGTCTCCTCCCCATAAGAAAG
PRIMER_LEFT_41=622,20
PRIMER_RIGHT_41=1589,21
PRIMER_LEFT_41_TM=60.232
PRIMER_RIGHT_41_TM=58.253
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=968
PRIMER_PAIR_42_PENALTY=2.979067
PRIMER_LEFT_42_PENALTY=0.232299
PRIMER_RIGHT_42_PENALTY=2.746768
PRIMER_LEFT_42_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_42_SEQUENCE=CTTGTCTCCTCCCCATAAGAA
PRIMER_LEFT_42=622,20
PRIMER_RIGHT_42=1591,21
PRIMER_LEFT_42_TM=60.232
PRIMER_RIGHT_42_TM=58.253
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=970
PRIMER_PAIR_43_PENALTY=2.995331
PRIMER_LEFT_43_PENALTY=0.998653
PRIMER_RIGHT_43_PENALTY=1.996678
PRIMER_LEFT_43_SEQUENCE=GCATCAGCATCCCCTTATTT
PRIMER_RIGHT_43_SEQUENCE=TCATGGTACTTGTCTCCTCCC
PRIMER_LEFT_43=602,20
PRIMER_RIGHT_43=1599,21
PRIMER_LEFT_43_TM=59.001
PRIMER_RIGHT_43_TM=59.003
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.2000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=998
PRIMER_PAIR_44_PENALTY=2.999753
PRIMER_LEFT_44_PENALTY=0.232299
PRIMER_RIGHT_44_PENALTY=2.767453
PRIMER_LEFT_44_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_44_SEQUENCE=CCTCCCCATAAGAAAGAGTGAG
PRIMER_LEFT_44=622,20
PRIMER_RIGHT_44=1584,22
PRIMER_LEFT_44_TM=60.232
PRIMER_RIGHT_44_TM=59.233
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=963
PRIMER_PAIR_45_PENALTY=2.999753
PRIMER_LEFT_45_PENALTY=0.232299
PRIMER_RIGHT_45_PENALTY=2.767453
PRIMER_LEFT_45_SEQUENCE=TGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_45_SEQUENCE=CTCCTCCCCATAAGAAAGAGTG
PRIMER_LEFT_45=622,20
PRIMER_RIGHT_45=1586,22
PRIMER_LEFT_45_TM=60.232
PRIMER_RIGHT_45_TM=59.233
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=965
PRIMER_PAIR_46_PENALTY=3.017480
PRIMER_LEFT_46_PENALTY=2.516652
PRIMER_RIGHT_46_PENALTY=0.500828
PRIMER_LEFT_46_SEQUENCE=ACAAAAGAATGTGTGGGATGGT
PRIMER_RIGHT_46_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_46=908,22
PRIMER_RIGHT_46=1596,20
PRIMER_LEFT_46_TM=60.517
PRIMER_RIGHT_46_TM=60.501
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.8000
PRIMER_RIGHT_46_END_STABILITY=11.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=689
PRIMER_PAIR_47_PENALTY=3.024095
PRIMER_LEFT_47_PENALTY=2.523267
PRIMER_RIGHT_47_PENALTY=0.500828
PRIMER_LEFT_47_SEQUENCE=TTGTTTCCAAGTCAGGCTTCC
PRIMER_RIGHT_47_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_47=621,21
PRIMER_RIGHT_47=1596,20
PRIMER_LEFT_47_TM=61.523
PRIMER_RIGHT_47_TM=60.501
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=11.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=976
PRIMER_PAIR_48_PENALTY=3.027939
PRIMER_LEFT_48_PENALTY=2.527111
PRIMER_RIGHT_48_PENALTY=0.500828
PRIMER_LEFT_48_SEQUENCE=AGCATCCCCTTATTTTGTTTCC
PRIMER_RIGHT_48_SEQUENCE=TGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_48=607,22
PRIMER_RIGHT_48=1596,20
PRIMER_LEFT_48_TM=60.527
PRIMER_RIGHT_48_TM=60.501
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=11.2000
PRIMER_PAIR_48_COMPL_ANY=2.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=990
PRIMER_PAIR_49_PENALTY=3.044490
PRIMER_LEFT_49_PENALTY=1.293639
PRIMER_RIGHT_49_PENALTY=1.750851
PRIMER_LEFT_49_SEQUENCE=GCATCAGCATCCCCTTATTTT
PRIMER_RIGHT_49_SEQUENCE=ATGGTACTTGTCTCCTCCCCA
PRIMER_LEFT_49=602,21
PRIMER_RIGHT_49=1597,21
PRIMER_LEFT_49_TM=60.294
PRIMER_RIGHT_49_TM=60.751
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.2000
PRIMER_RIGHT_49_END_STABILITY=11.2000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=996
=
SEQUENCE_ID=MAGIC.105_4_10814158_10814425_po-0_ler-0_38957_4574_TRUE
SEQUENCE_TARGET=950,367
SEQUENCE_TEMPLATE=AATGTACATATGCAAGNNNNNNNNNNNNNNANAAAAAGAACGCANAAAAAGAAAAAGAAAANNNGAGAGAGAAANAAAGAAATCGAGTTTAACTGCTCGTGTCTCTCTNTGCCACTCGTCTTCAGTTTTGTCAGCGTCTTCATAACTTATAAAGTCCTATCGATCTCTCTTNTTCTTTCTTTGGCCACTCTCTGTGTCTTTCTTTGTTAATAATATCTCAAAGAATAANNAAAAAAAAAANAAAAGNNNNNNNAAAANCACAAGTTTNTTGAAACCGTGTTNTTTTCGTGTAATATACANAGTTGTTACAGATTCTCTCCTTTTGGGTTTCTTTTTGNNATCATCATTNGATTGACTGATTCTCTGTTCNTTTTTTCCCANAAAGAGAGTCCTTTTATTANAAAAAAANNATTAAGGTTCTGTTTTGTTTNGTTTAGCNTTTTTATCCAATTNCATCAGAACCAGAGTTGTTTNTTGCTGCTCTGTNATATACTCGAGTCAATNTTTATANTNANGNNAAAAAAAAGTTTCNATNTTTGGTTTCATCTCANGCGNAAANTGCTNCAAATGGTAATCGATTTCTAGGNCTCTGACTGNTTTTGNTTAAAGGAATCAATTCAGGGNNNNNNNNNNNNNNNTTGTTACNAAAATGGCGGAAAGNGTNGAANCATCATCTGTACCAGAAGGAGAAAATACAATAGAAGAAAGAGAAGTAGGAGCTATGTGGGAATTAGANCAGAAACTTGATCAACCCATGGATGAAGAAGCTAATAAGCTCAACAACATGTATAGAGAAAAGGTNNTTACTTTCTCATTNAAATTCATNTGTAGATTTTGAGAAATGNAGNAAAAGTTGANGNTTTTAAAGTTTGCTCTNTTTTTTNTTTNATCAGGGNTTATCAATGTTGATGCTTCTAAGGCTNTCTTTTCAAAGTCTNGGGATAGTTTATGGAGATTTAGGGACNTCTCCNTTGTATGTGTTNTACAATACATTCCCTGANGGNATTGATGATAGTGAAGATGTAATCGGAGCTCTTTCTTTGATCATTTACTCTCTTTTNCTTATTCCTCTCATCAAGTATGTGTTCATTGTCTGCAAAGCTAATGATAATGGTCAAGGTAAGGTTTAGACCGCGGTGTTTTGAACNNANCGGTTTTGGTNTATNCTCATTCNGANTGATNAATGTTNNTNTGCAGGTGGGACTTTAGCTATATANTCGTTGCTTTGTAGACATGCTAAAGTGAAACTAATNCCGAATCAGCACCGNAGTGATGAGGATCTCACGACTTATAGTCGAACTGTATCCGCTGAAGGGTCTTTTGCTGCTAAAACCAAGAANTGGTTGGAGGGTAAAGANTGGAGGAAGAGAGCTCTTCTTGTCGTTGTTCTTCTCGGTACTTGTATGATGATCGGTGATGGTATCTTAACTCCAGCTATCTCCGGTAAAGCTTCTTNNCTTTNTTTCNNCTTNAGGTTNANCAAAAAAGATGATAATNGTTTGCAAAANCANCNTCTTACAGTTCTTTCAGCAACTGGTGGGATCAAAGTNAACAATCCAAAAATGAGCGGCGGTAACATTCNCNNNNNNNNNNNNNNNNNNNNNNGTTTCGAATCNGTTCTTGTTTTGCTAACTTGCTACTTTTCCNGTTTGCAGATATCGTCGTGCTTGTGGCAATTGNCATTTTNATAGGATTGTTNAGTATGCAACACTANGGTACAGACAAAGTCGGATGGCTCTTTGCNCCTATNGTGCTTATTTGGTTCCTCTTCATTGGAGCCACTGGGATGTACAACATCTGCAAGTATGACACAAGNGTTTTAAAAGCGTTTTCGCCTACATATATATACTTGTACTTCAAAAGACGAGGTCGAGATGGTTGGATATCGCTCGGTGGCATTCTTCTCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANAGCCATNTCTAAATGTTACTTCTTGAATATTGCAGGCACNGAGGCACTATACGCAGACATTGCNTATTTCCCNTTACTAGCGATACAGCTCGCCTTTACGTTTTTTGTGTTCCCTTGTCTTCTTNTAGCATACTGTGGACAAGCTGCNTATCTTGTGANTCATAAGGAACATTATCAAGACGCGTTCTATGCATCTATCCCGGGTTTGCCNTAAATCTTTTNAAAACATTACTGGTCTTGAAGNNNTCATNCNTAGTGCNNTTATAAAACANTNNANCTNTNNTTTGCTTTGACTCCNCAGATAGTGTCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.320611
PRIMER_LEFT_0_PENALTY=1.258650
PRIMER_RIGHT_0_PENALTY=0.061961
PRIMER_LEFT_0_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_0_SEQUENCE=CCGCTCATTTTTGGATTGTT
PRIMER_LEFT_0=745,21
PRIMER_RIGHT_0=1569,20
PRIMER_LEFT_0_TM=59.741
PRIMER_RIGHT_0_TM=59.938
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=825
PRIMER_PAIR_1_PENALTY=1.369727
PRIMER_LEFT_1_PENALTY=1.258650
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_1_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_1=745,21
PRIMER_RIGHT_1=1542,20
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=798
PRIMER_PAIR_2_PENALTY=1.531317
PRIMER_LEFT_2_PENALTY=1.258650
PRIMER_RIGHT_2_PENALTY=0.272667
PRIMER_LEFT_2_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_2_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_2=745,21
PRIMER_RIGHT_2=1736,20
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=59.727
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=992
PRIMER_PAIR_3_PENALTY=1.574671
PRIMER_LEFT_3_PENALTY=1.512710
PRIMER_RIGHT_3_PENALTY=0.061961
PRIMER_LEFT_3_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=CCGCTCATTTTTGGATTGTT
PRIMER_LEFT_3=747,20
PRIMER_RIGHT_3=1569,20
PRIMER_LEFT_3_TM=58.487
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=823
PRIMER_PAIR_4_PENALTY=1.623787
PRIMER_LEFT_4_PENALTY=1.512710
PRIMER_RIGHT_4_PENALTY=0.111077
PRIMER_LEFT_4_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_4_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_4=747,20
PRIMER_RIGHT_4=1542,20
PRIMER_LEFT_4_TM=58.487
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=796
PRIMER_PAIR_5_PENALTY=1.785377
PRIMER_LEFT_5_PENALTY=1.512710
PRIMER_RIGHT_5_PENALTY=0.272667
PRIMER_LEFT_5_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_5_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_5=747,20
PRIMER_RIGHT_5=1736,20
PRIMER_LEFT_5_TM=58.487
PRIMER_RIGHT_5_TM=59.727
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=990
PRIMER_PAIR_6_PENALTY=1.837000
PRIMER_LEFT_6_PENALTY=1.725923
PRIMER_RIGHT_6_PENALTY=0.111077
PRIMER_LEFT_6_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_6_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_6=745,20
PRIMER_RIGHT_6=1542,20
PRIMER_LEFT_6_TM=58.274
PRIMER_RIGHT_6_TM=60.111
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=798
PRIMER_PAIR_7_PENALTY=1.998591
PRIMER_LEFT_7_PENALTY=1.725923
PRIMER_RIGHT_7_PENALTY=0.272667
PRIMER_LEFT_7_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_7_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_7=745,20
PRIMER_RIGHT_7=1736,20
PRIMER_LEFT_7_TM=58.274
PRIMER_RIGHT_7_TM=59.727
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=992
PRIMER_PAIR_8_PENALTY=2.145179
PRIMER_LEFT_8_PENALTY=2.034102
PRIMER_RIGHT_8_PENALTY=0.111077
PRIMER_LEFT_8_SEQUENCE=AACCCATGGATGAAGAAGCTAA
PRIMER_RIGHT_8_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_8=749,22
PRIMER_RIGHT_8=1542,20
PRIMER_LEFT_8_TM=59.966
PRIMER_RIGHT_8_TM=60.111
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.5000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=794
PRIMER_PAIR_9_PENALTY=2.261228
PRIMER_LEFT_9_PENALTY=2.150151
PRIMER_RIGHT_9_PENALTY=0.111077
PRIMER_LEFT_9_SEQUENCE=ATCAACCCATGGATGAAGAAG
PRIMER_RIGHT_9_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_9=746,21
PRIMER_RIGHT_9=1542,20
PRIMER_LEFT_9_TM=58.850
PRIMER_RIGHT_9_TM=60.111
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=797
PRIMER_PAIR_10_PENALTY=2.287023
PRIMER_LEFT_10_PENALTY=2.225062
PRIMER_RIGHT_10_PENALTY=0.061961
PRIMER_LEFT_10_SEQUENCE=ATCAACCCATGGATGAAGAA
PRIMER_RIGHT_10_SEQUENCE=CCGCTCATTTTTGGATTGTT
PRIMER_LEFT_10=746,20
PRIMER_RIGHT_10=1569,20
PRIMER_LEFT_10_TM=57.775
PRIMER_RIGHT_10_TM=59.938
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=824
PRIMER_PAIR_11_PENALTY=2.306769
PRIMER_LEFT_11_PENALTY=2.034102
PRIMER_RIGHT_11_PENALTY=0.272667
PRIMER_LEFT_11_SEQUENCE=AACCCATGGATGAAGAAGCTAA
PRIMER_RIGHT_11_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_11=749,22
PRIMER_RIGHT_11=1736,20
PRIMER_LEFT_11_TM=59.966
PRIMER_RIGHT_11_TM=59.727
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.5000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=988
PRIMER_PAIR_12_PENALTY=2.336139
PRIMER_LEFT_12_PENALTY=2.225062
PRIMER_RIGHT_12_PENALTY=0.111077
PRIMER_LEFT_12_SEQUENCE=ATCAACCCATGGATGAAGAA
PRIMER_RIGHT_12_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_12=746,20
PRIMER_RIGHT_12=1542,20
PRIMER_LEFT_12_TM=57.775
PRIMER_RIGHT_12_TM=60.111
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=797
PRIMER_PAIR_13_PENALTY=2.346301
PRIMER_LEFT_13_PENALTY=2.235224
PRIMER_RIGHT_13_PENALTY=0.111077
PRIMER_LEFT_13_SEQUENCE=CCCATGGATGAAGAAGCTAA
PRIMER_RIGHT_13_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_13=751,20
PRIMER_RIGHT_13=1542,20
PRIMER_LEFT_13_TM=57.765
PRIMER_RIGHT_13_TM=60.111
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.5000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=792
PRIMER_PAIR_14_PENALTY=2.364367
PRIMER_LEFT_14_PENALTY=1.258650
PRIMER_RIGHT_14_PENALTY=1.105717
PRIMER_LEFT_14_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_14_SEQUENCE=AGAGCCATCCGACTTTGTCT
PRIMER_LEFT_14=745,21
PRIMER_RIGHT_14=1738,20
PRIMER_LEFT_14_TM=59.741
PRIMER_RIGHT_14_TM=58.894
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=994
PRIMER_PAIR_15_PENALTY=2.422818
PRIMER_LEFT_15_PENALTY=2.150151
PRIMER_RIGHT_15_PENALTY=0.272667
PRIMER_LEFT_15_SEQUENCE=ATCAACCCATGGATGAAGAAG
PRIMER_RIGHT_15_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_15=746,21
PRIMER_RIGHT_15=1736,20
PRIMER_LEFT_15_TM=58.850
PRIMER_RIGHT_15_TM=59.727
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=991
PRIMER_PAIR_16_PENALTY=2.452109
PRIMER_LEFT_16_PENALTY=2.341032
PRIMER_RIGHT_16_PENALTY=0.111077
PRIMER_LEFT_16_SEQUENCE=ACCCATGGATGAAGAAGCTAA
PRIMER_RIGHT_16_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_16=750,21
PRIMER_RIGHT_16=1542,20
PRIMER_LEFT_16_TM=58.659
PRIMER_RIGHT_16_TM=60.111
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.5000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=793
PRIMER_PAIR_17_PENALTY=2.462552
PRIMER_LEFT_17_PENALTY=1.258650
PRIMER_RIGHT_17_PENALTY=1.203901
PRIMER_LEFT_17_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_17_SEQUENCE=CCCACCAGTTGCTGAAAGAA
PRIMER_LEFT_17=745,21
PRIMER_RIGHT_17=1540,20
PRIMER_LEFT_17_TM=59.741
PRIMER_RIGHT_17_TM=61.204
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=796
PRIMER_PAIR_18_PENALTY=2.466882
PRIMER_LEFT_18_PENALTY=1.258650
PRIMER_RIGHT_18_PENALTY=1.208232
PRIMER_LEFT_18_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_18_SEQUENCE=AAAGAGCCATCCGACTTTGT
PRIMER_LEFT_18=745,21
PRIMER_RIGHT_18=1740,20
PRIMER_LEFT_18_TM=59.741
PRIMER_RIGHT_18_TM=58.792
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=996
PRIMER_PAIR_19_PENALTY=2.497729
PRIMER_LEFT_19_PENALTY=2.225062
PRIMER_RIGHT_19_PENALTY=0.272667
PRIMER_LEFT_19_SEQUENCE=ATCAACCCATGGATGAAGAA
PRIMER_RIGHT_19_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_19=746,20
PRIMER_RIGHT_19=1736,20
PRIMER_LEFT_19_TM=57.775
PRIMER_RIGHT_19_TM=59.727
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=991
PRIMER_PAIR_20_PENALTY=2.507891
PRIMER_LEFT_20_PENALTY=2.235224
PRIMER_RIGHT_20_PENALTY=0.272667
PRIMER_LEFT_20_SEQUENCE=CCCATGGATGAAGAAGCTAA
PRIMER_RIGHT_20_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_20=751,20
PRIMER_RIGHT_20=1736,20
PRIMER_LEFT_20_TM=57.765
PRIMER_RIGHT_20_TM=59.727
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.5000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=986
PRIMER_PAIR_21_PENALTY=2.519876
PRIMER_LEFT_21_PENALTY=1.258650
PRIMER_RIGHT_21_PENALTY=1.261226
PRIMER_LEFT_21_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_21_SEQUENCE=AAGAGCCATCCGACTTTGTCT
PRIMER_LEFT_21=745,21
PRIMER_RIGHT_21=1739,21
PRIMER_LEFT_21_TM=59.741
PRIMER_RIGHT_21_TM=60.261
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=995
PRIMER_PAIR_22_PENALTY=2.613699
PRIMER_LEFT_22_PENALTY=2.341032
PRIMER_RIGHT_22_PENALTY=0.272667
PRIMER_LEFT_22_SEQUENCE=ACCCATGGATGAAGAAGCTAA
PRIMER_RIGHT_22_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_22=750,21
PRIMER_RIGHT_22=1736,20
PRIMER_LEFT_22_TM=58.659
PRIMER_RIGHT_22_TM=59.727
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.5000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=987
PRIMER_PAIR_23_PENALTY=2.618427
PRIMER_LEFT_23_PENALTY=1.512710
PRIMER_RIGHT_23_PENALTY=1.105717
PRIMER_LEFT_23_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_23_SEQUENCE=AGAGCCATCCGACTTTGTCT
PRIMER_LEFT_23=747,20
PRIMER_RIGHT_23=1738,20
PRIMER_LEFT_23_TM=58.487
PRIMER_RIGHT_23_TM=58.894
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=992
PRIMER_PAIR_24_PENALTY=2.674993
PRIMER_LEFT_24_PENALTY=1.258650
PRIMER_RIGHT_24_PENALTY=1.416342
PRIMER_LEFT_24_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_24_SEQUENCE=CCGAGAAGAACAACGACAAGA
PRIMER_LEFT_24=745,21
PRIMER_RIGHT_24=1395,21
PRIMER_LEFT_24_TM=59.741
PRIMER_RIGHT_24_TM=60.416
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=651
PRIMER_PAIR_25_PENALTY=2.716611
PRIMER_LEFT_25_PENALTY=1.512710
PRIMER_RIGHT_25_PENALTY=1.203901
PRIMER_LEFT_25_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_25_SEQUENCE=CCCACCAGTTGCTGAAAGAA
PRIMER_LEFT_25=747,20
PRIMER_RIGHT_25=1540,20
PRIMER_LEFT_25_TM=58.487
PRIMER_RIGHT_25_TM=61.204
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=794
PRIMER_PAIR_26_PENALTY=2.720941
PRIMER_LEFT_26_PENALTY=1.512710
PRIMER_RIGHT_26_PENALTY=1.208232
PRIMER_LEFT_26_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_26_SEQUENCE=AAAGAGCCATCCGACTTTGT
PRIMER_LEFT_26=747,20
PRIMER_RIGHT_26=1740,20
PRIMER_LEFT_26_TM=58.487
PRIMER_RIGHT_26_TM=58.792
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=994
PRIMER_PAIR_27_PENALTY=2.763065
PRIMER_LEFT_27_PENALTY=1.258650
PRIMER_RIGHT_27_PENALTY=1.504414
PRIMER_LEFT_27_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_27_SEQUENCE=CCGAGAAGAACAACGACAAG
PRIMER_LEFT_27=745,21
PRIMER_RIGHT_27=1395,20
PRIMER_LEFT_27_TM=59.741
PRIMER_RIGHT_27_TM=58.496
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=651
PRIMER_PAIR_28_PENALTY=2.773935
PRIMER_LEFT_28_PENALTY=1.512710
PRIMER_RIGHT_28_PENALTY=1.261226
PRIMER_LEFT_28_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_28_SEQUENCE=AAGAGCCATCCGACTTTGTCT
PRIMER_LEFT_28=747,20
PRIMER_RIGHT_28=1739,21
PRIMER_LEFT_28_TM=58.487
PRIMER_RIGHT_28_TM=60.261
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=993
PRIMER_PAIR_29_PENALTY=2.803775
PRIMER_LEFT_29_PENALTY=2.692698
PRIMER_RIGHT_29_PENALTY=0.111077
PRIMER_LEFT_29_SEQUENCE=GATCAACCCATGGATGAAGAAG
PRIMER_RIGHT_29_SEQUENCE=ATCCCACCAGTTGCTGAAAG
PRIMER_LEFT_29=745,22
PRIMER_RIGHT_29=1542,20
PRIMER_LEFT_29_TM=60.693
PRIMER_RIGHT_29_TM=60.111
PRIMER_LEFT_29_GC_PERCENT=45.455
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=798
PRIMER_PAIR_30_PENALTY=2.831640
PRIMER_LEFT_30_PENALTY=1.725923
PRIMER_RIGHT_30_PENALTY=1.105717
PRIMER_LEFT_30_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_30_SEQUENCE=AGAGCCATCCGACTTTGTCT
PRIMER_LEFT_30=745,20
PRIMER_RIGHT_30=1738,20
PRIMER_LEFT_30_TM=58.274
PRIMER_RIGHT_30_TM=58.894
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=994
PRIMER_PAIR_31_PENALTY=2.872230
PRIMER_LEFT_31_PENALTY=1.258650
PRIMER_RIGHT_31_PENALTY=1.613579
PRIMER_LEFT_31_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_31_SEQUENCE=ACCGAGAAGAACAACGACAAG
PRIMER_LEFT_31=745,21
PRIMER_RIGHT_31=1396,21
PRIMER_LEFT_31_TM=59.741
PRIMER_RIGHT_31_TM=59.386
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=652
PRIMER_PAIR_32_PENALTY=2.916160
PRIMER_LEFT_32_PENALTY=1.258650
PRIMER_RIGHT_32_PENALTY=1.657509
PRIMER_LEFT_32_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_32_SEQUENCE=ACCGAGAAGAACAACGACAA
PRIMER_LEFT_32=745,21
PRIMER_RIGHT_32=1396,20
PRIMER_LEFT_32_TM=59.741
PRIMER_RIGHT_32_TM=58.342
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=2.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=652
PRIMER_PAIR_33_PENALTY=2.929052
PRIMER_LEFT_33_PENALTY=1.512710
PRIMER_RIGHT_33_PENALTY=1.416342
PRIMER_LEFT_33_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_33_SEQUENCE=CCGAGAAGAACAACGACAAGA
PRIMER_LEFT_33=747,20
PRIMER_RIGHT_33=1395,21
PRIMER_LEFT_33_TM=58.487
PRIMER_RIGHT_33_TM=60.416
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=2.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=649
PRIMER_PAIR_34_PENALTY=2.929825
PRIMER_LEFT_34_PENALTY=1.725923
PRIMER_RIGHT_34_PENALTY=1.203901
PRIMER_LEFT_34_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_34_SEQUENCE=CCCACCAGTTGCTGAAAGAA
PRIMER_LEFT_34=745,20
PRIMER_RIGHT_34=1540,20
PRIMER_LEFT_34_TM=58.274
PRIMER_RIGHT_34_TM=61.204
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=796
PRIMER_PAIR_35_PENALTY=2.934155
PRIMER_LEFT_35_PENALTY=1.725923
PRIMER_RIGHT_35_PENALTY=1.208232
PRIMER_LEFT_35_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_35_SEQUENCE=AAAGAGCCATCCGACTTTGT
PRIMER_LEFT_35=745,20
PRIMER_RIGHT_35=1740,20
PRIMER_LEFT_35_TM=58.274
PRIMER_RIGHT_35_TM=58.792
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=996
PRIMER_PAIR_36_PENALTY=2.965365
PRIMER_LEFT_36_PENALTY=2.692698
PRIMER_RIGHT_36_PENALTY=0.272667
PRIMER_LEFT_36_SEQUENCE=GATCAACCCATGGATGAAGAAG
PRIMER_RIGHT_36_SEQUENCE=AGCCATCCGACTTTGTCTGT
PRIMER_LEFT_36=745,22
PRIMER_RIGHT_36=1736,20
PRIMER_LEFT_36_TM=60.693
PRIMER_RIGHT_36_TM=59.727
PRIMER_LEFT_36_GC_PERCENT=45.455
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=992
PRIMER_PAIR_37_PENALTY=2.975089
PRIMER_LEFT_37_PENALTY=1.258650
PRIMER_RIGHT_37_PENALTY=1.716438
PRIMER_LEFT_37_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_37_SEQUENCE=GCTTTACCGGAGATAGCTGGA
PRIMER_LEFT_37=745,21
PRIMER_RIGHT_37=1449,21
PRIMER_LEFT_37_TM=59.741
PRIMER_RIGHT_37_TM=60.716
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=705
PRIMER_PAIR_38_PENALTY=2.979032
PRIMER_LEFT_38_PENALTY=1.258650
PRIMER_RIGHT_38_PENALTY=1.720382
PRIMER_LEFT_38_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_38_SEQUENCE=ATGTTACCGCCGCTCATTTT
PRIMER_LEFT_38=745,21
PRIMER_RIGHT_38=1578,20
PRIMER_LEFT_38_TM=59.741
PRIMER_RIGHT_38_TM=61.720
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=834
PRIMER_PAIR_39_PENALTY=2.979032
PRIMER_LEFT_39_PENALTY=1.258650
PRIMER_RIGHT_39_PENALTY=1.720382
PRIMER_LEFT_39_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_39_SEQUENCE=AATGTTACCGCCGCTCATTT
PRIMER_LEFT_39=745,21
PRIMER_RIGHT_39=1579,20
PRIMER_LEFT_39_TM=59.741
PRIMER_RIGHT_39_TM=61.720
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=835
PRIMER_PAIR_40_PENALTY=2.987149
PRIMER_LEFT_40_PENALTY=1.725923
PRIMER_RIGHT_40_PENALTY=1.261226
PRIMER_LEFT_40_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_40_SEQUENCE=AAGAGCCATCCGACTTTGTCT
PRIMER_LEFT_40=745,20
PRIMER_RIGHT_40=1739,21
PRIMER_LEFT_40_TM=58.274
PRIMER_RIGHT_40_TM=60.261
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=995
PRIMER_PAIR_41_PENALTY=3.017124
PRIMER_LEFT_41_PENALTY=1.512710
PRIMER_RIGHT_41_PENALTY=1.504414
PRIMER_LEFT_41_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_41_SEQUENCE=CCGAGAAGAACAACGACAAG
PRIMER_LEFT_41=747,20
PRIMER_RIGHT_41=1395,20
PRIMER_LEFT_41_TM=58.487
PRIMER_RIGHT_41_TM=58.496
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=649
PRIMER_PAIR_42_PENALTY=3.035415
PRIMER_LEFT_42_PENALTY=1.258650
PRIMER_RIGHT_42_PENALTY=1.776765
PRIMER_LEFT_42_SEQUENCE=GATCAACCCATGGATGAAGAA
PRIMER_RIGHT_42_SEQUENCE=TCCCACCAGTTGCTGAAAGA
PRIMER_LEFT_42=745,21
PRIMER_RIGHT_42=1541,20
PRIMER_LEFT_42_TM=59.741
PRIMER_RIGHT_42_TM=61.777
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=797
PRIMER_PAIR_43_PENALTY=3.126289
PRIMER_LEFT_43_PENALTY=1.512710
PRIMER_RIGHT_43_PENALTY=1.613579
PRIMER_LEFT_43_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_43_SEQUENCE=ACCGAGAAGAACAACGACAAG
PRIMER_LEFT_43=747,20
PRIMER_RIGHT_43=1396,21
PRIMER_LEFT_43_TM=58.487
PRIMER_RIGHT_43_TM=59.386
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=650
PRIMER_PAIR_44_PENALTY=3.139819
PRIMER_LEFT_44_PENALTY=2.034102
PRIMER_RIGHT_44_PENALTY=1.105717
PRIMER_LEFT_44_SEQUENCE=AACCCATGGATGAAGAAGCTAA
PRIMER_RIGHT_44_SEQUENCE=AGAGCCATCCGACTTTGTCT
PRIMER_LEFT_44=749,22
PRIMER_RIGHT_44=1738,20
PRIMER_LEFT_44_TM=59.966
PRIMER_RIGHT_44_TM=58.894
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.5000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=990
PRIMER_PAIR_45_PENALTY=3.142266
PRIMER_LEFT_45_PENALTY=1.725923
PRIMER_RIGHT_45_PENALTY=1.416342
PRIMER_LEFT_45_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_45_SEQUENCE=CCGAGAAGAACAACGACAAGA
PRIMER_LEFT_45=745,20
PRIMER_RIGHT_45=1395,21
PRIMER_LEFT_45_TM=58.274
PRIMER_RIGHT_45_TM=60.416
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=651
PRIMER_PAIR_46_PENALTY=3.170219
PRIMER_LEFT_46_PENALTY=1.512710
PRIMER_RIGHT_46_PENALTY=1.657509
PRIMER_LEFT_46_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_46_SEQUENCE=ACCGAGAAGAACAACGACAA
PRIMER_LEFT_46=747,20
PRIMER_RIGHT_46=1396,20
PRIMER_LEFT_46_TM=58.487
PRIMER_RIGHT_46_TM=58.342
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=650
PRIMER_PAIR_47_PENALTY=3.229148
PRIMER_LEFT_47_PENALTY=1.512710
PRIMER_RIGHT_47_PENALTY=1.716438
PRIMER_LEFT_47_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_47_SEQUENCE=GCTTTACCGGAGATAGCTGGA
PRIMER_LEFT_47=747,20
PRIMER_RIGHT_47=1449,21
PRIMER_LEFT_47_TM=58.487
PRIMER_RIGHT_47_TM=60.716
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=703
PRIMER_PAIR_48_PENALTY=3.230338
PRIMER_LEFT_48_PENALTY=1.725923
PRIMER_RIGHT_48_PENALTY=1.504414
PRIMER_LEFT_48_SEQUENCE=GATCAACCCATGGATGAAGA
PRIMER_RIGHT_48_SEQUENCE=CCGAGAAGAACAACGACAAG
PRIMER_LEFT_48=745,20
PRIMER_RIGHT_48=1395,20
PRIMER_LEFT_48_TM=58.274
PRIMER_RIGHT_48_TM=58.496
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=2.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=651
PRIMER_PAIR_49_PENALTY=3.233092
PRIMER_LEFT_49_PENALTY=1.512710
PRIMER_RIGHT_49_PENALTY=1.720382
PRIMER_LEFT_49_SEQUENCE=TCAACCCATGGATGAAGAAG
PRIMER_RIGHT_49_SEQUENCE=ATGTTACCGCCGCTCATTTT
PRIMER_LEFT_49=747,20
PRIMER_RIGHT_49=1578,20
PRIMER_LEFT_49_TM=58.487
PRIMER_RIGHT_49_TM=61.720
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=832
=
SEQUENCE_ID=MAGIC.433_4_4763742_4763996_wu-0_oy-0_9636_184192_TRUE
SEQUENCE_TARGET=950,354
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_3093824_3094159_oy-0_kn-0_168128_62532_TRUE
SEQUENCE_TARGET=950,435
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACTGCANCNGANCCNCATGCCNCCTNTANNNAAGAAAACTTGGTTAAAGAGGAAGAGATGAGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGNTGACAATGACGAAGGANCCACCATTAGNGTTGTCATTNNGGTCTTAAAACCANGTGCTTTTTNATAATCGTGTTGANTCCANCTGCTGATNAATGAGATGANGATTNNNCGANAAATAATCTNAGCTTTTNNNNNNNNAANAAAAAAGAATCAAACCTAATTTTTGGTTTTCGAGTTGAACCATGTAAACTAGGTGANNTTCATTCTTANNNNGTCACAAATGCNAGATANNAGCCNTNATGTCTTTNTTAAANNGGGATAAACNNGTANNTTTGGGNCACTCAATTCACATGNTTNAGATGGTTATTTGTGGTTTTCTATCNGGTTTTATCTGGATCAATATGTTTGTTGACGGTACTACTCTNAAGCCAATATNTCATAAACACAAATCAAGAACTCAAACAAACCATAAGAAAACTCAGTAGNTTGTTTTATTTACTNTTNAAAAAGAAACATAAANAAAATTGGGAATNTACTTTTCACATAATGAACNAATAGCAAGTAANAAACATTACCATCATCATCTACANATATCTNCCCATCTTAATTCTCTATAGCAACCCTNNATTTANCAGAAGANGCATTCCTACAAAGAACCTATAAAAACTTGTGNAGAGAATCAAAAATGAANGTAAAAGGTCNNACCGAGGTTGTGATATCATTGTATTTAGGACGTNGTNGATCCGTAGATATCACTTNTTCCATTGCTTTTCTCTTATTACTACTCAACATTGGTTCAAGTTGTTCCGATTCAACTTCTTNGTGGAGAGGCAANATNTCAATCACTNATGACTCTTCTTCTATCTCCGGTTTAGAAACCACAATCTCTTCTATGGCAACTGGTTTCTCACTTTCTTTCTTNAACTTTGTTTTCATCATATGAGGTTTCAACTTGGTANGATCANCTAACTTTACTGGTTTCAAGAAAAACTCTTCAGCTCCTTNTTCNAAACATCTGTTGGAAGAAACCACCACAANTTTGTNCATAGTTCAAAGACTTATTCAAANCAAAGAATCAAATAANACCCACNTGCCAATTNAAAAATCAATAAGACTCATATTAATGTTAAATAANCAGAGCTCTTANNTGGAGATTCTTGTAGGAACGTTCTCAGATGANATTANAACTACANGTNTACTTCTAAATGCNGCTGATTCCTATACACAACNATCATGTNAATTAATCAGAAGNAAATAAAANTGAAGNNTTTATNTTGGGCTTGTCCAAAAGCNGNCCATAATNAAATGGACATCGACANTNCATGATTTTTAATATTGAACTTNTGAAGTATTNAACTTATGTTNTTNANAAGTTCTTAATATTGANCTTATGAAGTATTGAAATTATGTCANGTNTCATTGTGNTAAACAATTAAAANTTAAGTATGAAGTTTTGAATAAATGTNTTCATGNTATCCATGTATTANTTTGGACTCATGGACNGCCCAANTNACTATGGTTCTTTTTNAATTTGGTGTAATTTGGGTCTGNTCCTATTTGGGCTTAGACCAACAATGTCCAAAAAATGGGGTTGTAATGGANAGCCCATTTGACATCCCTAGCCATCATCGACGAAGCAAAATCCAGCAATTGAGAGAGNAGGAGTTGAAGAACCAANCCTGACCATAAGTNCGGTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=20
PRIMER_RIGHT_NUM_RETURNED=20
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=20
PRIMER_PAIR_0_PENALTY=6.927605
PRIMER_LEFT_0_PENALTY=2.794652
PRIMER_RIGHT_0_PENALTY=4.132953
PRIMER_LEFT_0_SEQUENCE=GGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_0_SEQUENCE=AACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_0=691,22
PRIMER_RIGHT_0=1467,22
PRIMER_LEFT_0_TM=59.205
PRIMER_RIGHT_0_TM=57.867
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=777
PRIMER_PAIR_1_PENALTY=7.393048
PRIMER_LEFT_1_PENALTY=2.794652
PRIMER_RIGHT_1_PENALTY=4.598396
PRIMER_LEFT_1_SEQUENCE=GGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_1_SEQUENCE=ACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_1=691,22
PRIMER_RIGHT_1=1466,21
PRIMER_LEFT_1_TM=59.205
PRIMER_RIGHT_1_TM=56.402
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=776
PRIMER_PAIR_2_PENALTY=8.733421
PRIMER_LEFT_2_PENALTY=2.794652
PRIMER_RIGHT_2_PENALTY=5.938769
PRIMER_LEFT_2_SEQUENCE=GGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_2_SEQUENCE=AACGTTCCTACAAGAATCTCC
PRIMER_LEFT_2=691,22
PRIMER_RIGHT_2=1467,21
PRIMER_LEFT_2_TM=59.205
PRIMER_RIGHT_2_TM=55.061
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=777
PRIMER_PAIR_3_PENALTY=10.260555
PRIMER_LEFT_3_PENALTY=6.276397
PRIMER_RIGHT_3_PENALTY=3.984159
PRIMER_LEFT_3_SEQUENCE=CCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_3_SEQUENCE=CAGACCCAAATTACACCAAA
PRIMER_LEFT_3=896,25
PRIMER_RIGHT_3=1847,20
PRIMER_LEFT_3_TM=58.724
PRIMER_RIGHT_3_TM=56.016
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=952
PRIMER_PAIR_4_PENALTY=10.260555
PRIMER_LEFT_4_PENALTY=6.276397
PRIMER_RIGHT_4_PENALTY=3.984159
PRIMER_LEFT_4_SEQUENCE=CCCATCTTAATTCTCTATAGCAACC
PRIMER_RIGHT_4_SEQUENCE=CAGACCCAAATTACACCAAA
PRIMER_LEFT_4=895,25
PRIMER_RIGHT_4=1847,20
PRIMER_LEFT_4_TM=58.724
PRIMER_RIGHT_4_TM=56.016
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=953
PRIMER_PAIR_5_PENALTY=10.409349
PRIMER_LEFT_5_PENALTY=6.276397
PRIMER_RIGHT_5_PENALTY=4.132953
PRIMER_LEFT_5_SEQUENCE=CCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_5_SEQUENCE=AACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_5=896,25
PRIMER_RIGHT_5=1467,22
PRIMER_LEFT_5_TM=58.724
PRIMER_RIGHT_5_TM=57.867
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=8.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=572
PRIMER_PAIR_6_PENALTY=10.748344
PRIMER_LEFT_6_PENALTY=6.615391
PRIMER_RIGHT_6_PENALTY=4.132953
PRIMER_LEFT_6_SEQUENCE=CTGGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_6_SEQUENCE=AACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_6=689,24
PRIMER_RIGHT_6=1467,22
PRIMER_LEFT_6_TM=62.615
PRIMER_RIGHT_6_TM=57.867
PRIMER_LEFT_6_GC_PERCENT=41.667
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=779
PRIMER_PAIR_7_PENALTY=10.874793
PRIMER_LEFT_7_PENALTY=6.276397
PRIMER_RIGHT_7_PENALTY=4.598396
PRIMER_LEFT_7_SEQUENCE=CCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_7_SEQUENCE=ACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_7=896,25
PRIMER_RIGHT_7=1466,21
PRIMER_LEFT_7_TM=58.724
PRIMER_RIGHT_7_TM=56.402
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=8.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=571
PRIMER_PAIR_8_PENALTY=11.213787
PRIMER_LEFT_8_PENALTY=6.615391
PRIMER_RIGHT_8_PENALTY=4.598396
PRIMER_LEFT_8_SEQUENCE=CTGGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_8_SEQUENCE=ACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_8=689,24
PRIMER_RIGHT_8=1466,21
PRIMER_LEFT_8_TM=62.615
PRIMER_RIGHT_8_TM=56.402
PRIMER_LEFT_8_GC_PERCENT=41.667
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=778
PRIMER_PAIR_9_PENALTY=11.534220
PRIMER_LEFT_9_PENALTY=7.550061
PRIMER_RIGHT_9_PENALTY=3.984159
PRIMER_LEFT_9_SEQUENCE=CCCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_9_SEQUENCE=CAGACCCAAATTACACCAAA
PRIMER_LEFT_9=895,26
PRIMER_RIGHT_9=1847,20
PRIMER_LEFT_9_TM=61.550
PRIMER_RIGHT_9_TM=56.016
PRIMER_LEFT_9_GC_PERCENT=42.308
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=8.8000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=953
PRIMER_PAIR_10_PENALTY=11.683014
PRIMER_LEFT_10_PENALTY=7.550061
PRIMER_RIGHT_10_PENALTY=4.132953
PRIMER_LEFT_10_SEQUENCE=CCCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_10_SEQUENCE=AACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_10=895,26
PRIMER_RIGHT_10=1467,22
PRIMER_LEFT_10_TM=61.550
PRIMER_RIGHT_10_TM=57.867
PRIMER_LEFT_10_GC_PERCENT=42.308
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=8.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=573
PRIMER_PAIR_11_PENALTY=12.148457
PRIMER_LEFT_11_PENALTY=7.550061
PRIMER_RIGHT_11_PENALTY=4.598396
PRIMER_LEFT_11_SEQUENCE=CCCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_11_SEQUENCE=ACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_11=895,26
PRIMER_RIGHT_11=1466,21
PRIMER_LEFT_11_TM=61.550
PRIMER_RIGHT_11_TM=56.402
PRIMER_LEFT_11_GC_PERCENT=42.308
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=8.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=572
PRIMER_PAIR_12_PENALTY=12.215165
PRIMER_LEFT_12_PENALTY=6.276397
PRIMER_RIGHT_12_PENALTY=5.938769
PRIMER_LEFT_12_SEQUENCE=CCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_12_SEQUENCE=AACGTTCCTACAAGAATCTCC
PRIMER_LEFT_12=896,25
PRIMER_RIGHT_12=1467,21
PRIMER_LEFT_12_TM=58.724
PRIMER_RIGHT_12_TM=55.061
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=8.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=572
PRIMER_PAIR_13_PENALTY=12.554160
PRIMER_LEFT_13_PENALTY=6.615391
PRIMER_RIGHT_13_PENALTY=5.938769
PRIMER_LEFT_13_SEQUENCE=CTGGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_13_SEQUENCE=AACGTTCCTACAAGAATCTCC
PRIMER_LEFT_13=689,24
PRIMER_RIGHT_13=1467,21
PRIMER_LEFT_13_TM=62.615
PRIMER_RIGHT_13_TM=55.061
PRIMER_LEFT_13_GC_PERCENT=41.667
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=779
PRIMER_PAIR_14_PENALTY=13.198009
PRIMER_LEFT_14_PENALTY=9.213850
PRIMER_RIGHT_14_PENALTY=3.984159
PRIMER_LEFT_14_SEQUENCE=CCCATCTTAATTCTCTATAGCAACCCT
PRIMER_RIGHT_14_SEQUENCE=CAGACCCAAATTACACCAAA
PRIMER_LEFT_14=895,27
PRIMER_RIGHT_14=1847,20
PRIMER_LEFT_14_TM=62.214
PRIMER_RIGHT_14_TM=56.016
PRIMER_LEFT_14_GC_PERCENT=40.741
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=8.8000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=953
PRIMER_PAIR_15_PENALTY=13.287983
PRIMER_LEFT_15_PENALTY=9.155030
PRIMER_RIGHT_15_PENALTY=4.132953
PRIMER_LEFT_15_SEQUENCE=TCTGGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_15_SEQUENCE=AACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_15=688,25
PRIMER_RIGHT_15=1467,22
PRIMER_LEFT_15_TM=64.155
PRIMER_RIGHT_15_TM=57.867
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=780
PRIMER_PAIR_16_PENALTY=13.488830
PRIMER_LEFT_16_PENALTY=7.550061
PRIMER_RIGHT_16_PENALTY=5.938769
PRIMER_LEFT_16_SEQUENCE=CCCATCTTAATTCTCTATAGCAACCC
PRIMER_RIGHT_16_SEQUENCE=AACGTTCCTACAAGAATCTCC
PRIMER_LEFT_16=895,26
PRIMER_RIGHT_16=1467,21
PRIMER_LEFT_16_TM=61.550
PRIMER_RIGHT_16_TM=55.061
PRIMER_LEFT_16_GC_PERCENT=42.308
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=8.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=573
PRIMER_PAIR_17_PENALTY=13.753426
PRIMER_LEFT_17_PENALTY=9.155030
PRIMER_RIGHT_17_PENALTY=4.598396
PRIMER_LEFT_17_SEQUENCE=TCTGGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_17_SEQUENCE=ACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_17=688,25
PRIMER_RIGHT_17=1466,21
PRIMER_LEFT_17_TM=64.155
PRIMER_RIGHT_17_TM=56.402
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=779
PRIMER_PAIR_18_PENALTY=13.812246
PRIMER_LEFT_18_PENALTY=9.213850
PRIMER_RIGHT_18_PENALTY=4.598396
PRIMER_LEFT_18_SEQUENCE=CCCATCTTAATTCTCTATAGCAACCCT
PRIMER_RIGHT_18_SEQUENCE=ACGTTCCTACAAGAATCTCCA
PRIMER_LEFT_18=895,27
PRIMER_RIGHT_18=1466,21
PRIMER_LEFT_18_TM=62.214
PRIMER_RIGHT_18_TM=56.402
PRIMER_LEFT_18_GC_PERCENT=40.741
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.1000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=8.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=572
PRIMER_PAIR_19_PENALTY=15.093799
PRIMER_LEFT_19_PENALTY=9.155030
PRIMER_RIGHT_19_PENALTY=5.938769
PRIMER_LEFT_19_SEQUENCE=TCTGGATCAATATGTTTGTTGACGG
PRIMER_RIGHT_19_SEQUENCE=AACGTTCCTACAAGAATCTCC
PRIMER_LEFT_19=688,25
PRIMER_RIGHT_19=1467,21
PRIMER_LEFT_19_TM=64.155
PRIMER_RIGHT_19_TM=55.061
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=780
=
SEQUENCE_ID=MAGIC.446_4_2780200_2780227_zu-0_po-0_189637_36065_TRUE
SEQUENCE_TARGET=950,127
SEQUENCE_TEMPLATE=CCAAAAGGATTGAACNNAAANAAAANATATTACNAAAAGGAAAAGTTATTTACAATCTTNTTTCNTTTTATCACCTTATATNCTNTTCTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGATAGTCAATNATACAGTTAGGTGATCACGTAAACAATCATTAAGAGTNTNCTAGCTAGTGATTATTGATGATGNTGTGATTTGCATGGGGACACCATACCTNGTNTTTTGGACATTGTTGATANAAAGATCAAATGAAATTGGTGATGAAACACCAANGGAAAAGTGTNACACGNTTATATTCTCTNNNNNNCTACNGGTCTACTCAANATTTTNTTTNAGCTATCAATCGCAAGGCTTTGNATTGATGCTAAAATTAGTGGTTTTCATAAGTAAATTTAATTAATTATAATNTTATTANTNTTGATAGTATGTAACTAAANAATCGTGAAAGTAATGTACCAACTNAAAAAAATATAAAGACCCNTGNCATCNTTCTCGANCGACTTANAATCNCGCNCTCAAAACTTCTTNNAAAAGTTCAAAGNAAANNCNCTNANACTNNNNNNNNNNNNNNNNNNNNNNNTTCATAAGTCAAANAACAAAGGTGTCNATTGTCNAANNNANAAAANAAANNAACAACAACAAAGNTATNTCCGCAAGATTCAGAAAGATTNNATATCTAGTACCAATGATAGTCGGNNTCNTTANANTGTGACCCAAATGTTGTCACCGCACCNTNATNNNAGNCANCATCTGAANCGTCATCTGAGTCATTGGGGCANCTCAAGTCTTTGATNCTCTCAAAACATCGTTTCTTGAGATCCCCAACAAGGTTGATGTTGAAACACTGGCGTAAATCAAGGTGTTCNAGGTGAGGACAACCATCAAGAATGGCGTTCAAGCCCTTNTTGGTTAGCCCGTTCCCTAAAAGCTGGAGGTGGCGTAGTTCAGGCATGCTTTCTGCAATTGCTTTGGCATTATCATCAAACCCACTATTTGAAAACATGATTTCAGGGGCTCGGTTTAGCTTCAGTGTCTTCAGATNCGGACAAGACCGGCCTATATCTCTCAGAGACTCTCCTGAAAACAAGCAGTATGAAACCTCAAGGTATTCAAGCAACGGTACTTTCGAAACTGCTTTGGCAACTCCCTCATCTGTTATTGGAAAGCACCTTACAAGTCCAAGGCTTTTNAGATTACTTGACCTGCAAATANTTGTTACTAAAAAAGTCAAAAACATAACCTTATTGAGAACACTTCGTTGACAAAACCACTAAGCAAACCANGGTGCNTTATCAGANATTCAGTAGAAGGANATAGAANAGATAAAAATAAATCTAGTTTAAAAAGAGTGACACAAAATCNTCCAATCACTTATANCTNCANAATTAGCAATCACTCNAACCCTTGGATTAGCACCGATCACTCTTAGCCCNNNNNNTAGCAAACACTCTAACCCTAGTATTAGCANCNACCAAATTNNNTTTAACATGGAAAGAGAGCTAGNGAGAAACAGAACCTGTCNGCTATGTACNTGATGAGATCATTAGTACCNTAGTACCAAATCTCGATCTCAANCAAACCACCNTGGCTACGATCAACTGCGNGACGGCACATTGACTCGAGGTCGTACTTGAAGGCTNCCCTATCATTGCGGTTATCTATGTCGATCTTCCGCCACATCGAGGGGTCTTTGCAGACTCGATGCCACGGTTTGCACACTTTTTGAGCGTTTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_16092525_16092548_tsu-0_zu-0_8692_343066_TRUE
SEQUENCE_TARGET=950,123
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_3124694_3124776_ler-0_wil-2_428119_93793_TRUE
SEQUENCE_TARGET=950,182
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_4759124_4759180_edi-0_tsu-0_30600_32543_TRUE
SEQUENCE_TARGET=950,156
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_6379206_6379956_ler-0_ws-0_217047_366282_TRUE
SEQUENCE_TARGET=950,850
SEQUENCE_TEMPLATE=ATCCANANCCANTCTAATACTAGATCTAGCGGAATCAAACTCAAATGAAAATTAAANACCANTCGNAGAAGAAGATGGATAANTACTTTAGCTTGGTAGGCTTAGGCTTGCGTCTGTTAACAAGCGGCTTCTTNACGTCTTCCGCCGGATAAAACTTGGCAGGCTTCTCAACCGGAGCGTCGACCTTTGGCTGAGCATCGTGACGAGGGAAAACGCCACCGTTCTTGGCCTTGATAGCCCACAAACCTCTCTTGTGGTACATCTGAGACCTCGANTATTTTCCAACTCCCCTGATTAGGTCAGGGTTACGGTTAACCTTGGGAGTCCTCTTCGCNGCCGGCATTTTTTCGGCTTCGGTGGTNNAAAATCTATCAGAGNCATAAACCCTAGATTGAGNTTTTATATAGCAGAAANNAAAAACCTAATGGGCCCNTATGGTNNANAATNNTTTAATATGGGCTTNNTNTNNGNNTNTAGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCGTCGGCATCTNTTAGTGGCGTAAACCCTAANTGACAGTCGAAAGCTTTTAAATATAAGGACGATACCGGCNNNNNNAAAANCTAATGGGCCTTATATGGGTNTAATTCAACACNGCTNCTCTAATGCTTCCAAAANNAAACTCCATTCGATNAGGATTTGCTTCCCAGTTCAGGNAGAGGTGAATATTTTTCAAACTAACTCCAATAGTTTGGCATCTTCCNAGAGAGGAAACACNCCGNCTTAGCCACTAGAATATTTCCGACGTAGTTTGGTATCCGAANTAAANTTTCCTCAACTCCATCCATTGTANCTCTAAACACCCTAAATTGAGCTTTTANATAGCANAAANTAAAAAANCTAATGGGCCCTNATATGGTTNNNAAAANTTTAATATNGGGGTCNNNNNNNNNNTAGTNCNNAAGTGTTTTTATTGCAAAACATTGGGNCTTACATGAAGTATTTTTGTACTTACTATTTGGCNNGTAAGTAAAAGTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGNGTTCTCTNNTTNGCCGNCNGCATCTCTTAGTGGCGTAAACCNTAANTGACAGTCGAAAGNTTTTAAATATAANGACGATANCGGCNAAAATANNNNNTAATGGNCCTTATATGGGTNTAATGCAACANCGCTNCTCTAATGNTTCCAAAANGAAACTNNATTCGATNAGGANTTGCTTCCCAGTTNAGNNAGAGGTGAATNATTTTTCAAANTAACTCNAATANTTTGGCATNTTCCCAGANAGGAAACACCCCGCCTTAGCCACTANAATNTTTNNNCGTAGTTTGGNAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_7252216_7252582_edi-0_wil-2_147974_801595_TRUE
SEQUENCE_TARGET=950,466
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGTCTTTTGTTTTGTATCCTCTTTAAAAAAACATCGTTCCCTTAATTATTTACCTTGACTAGCTACATCCCTTGATGATGACTGCTATNTTTGTCCTNGA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_5_12705816_12706518_ws-0_ct-1_13107_382204_TRUE
SEQUENCE_TARGET=950,802
SEQUENCE_TEMPLATE=CAATNTCAATTGATGNGCTATTTAGTTTAATAANATGAAATGTTGNAGCCCATTAAACNAAGGAGAGCAAANTTATNAAAACGACAAGGGTTNNNNNNNNNNNNNTNGCCNTANATGNGTNTAAGCGTAANCATCTCCNNNNTNNTTTAGAAATGNCGCCAANAGAAGAAACGAGAGAGNGAGNGAGAGACAANNACNGAGACAAAGNAGGATCNTAGTGGGNAAAGTCACNATNGTNGCTTNNTNCGTGGNCNTNTTTNATTNAAGTTGTGGCCNGTGTTANCCGGAGTNGAGCNTTGTCATCATTGNNTTGTTGGGTAAGGAAANCTANCNTGNTTATGTCTTCTTCTTNTGNAANTTTNTGTTTTGNNNNNATANANNNNNNNTTTAAANCTNGGTTTTGTTNGTNNNGANGANGANGAGNAAACTGTGNCANGNAAGGGTTTNGAGGACTANTTNCCCGTNNAACCNAAGAGANTTACATCGAGNTTTTANNGTCNCNGGATANATCTNGNTGCAAGGANTCCANAGNANTTTACGGTTTATNAAACNGANGTATATTGCATNCANAATTCNTCTCGNAAGTTNNTGANAAATCANTNGTTCTGTTTTCAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTANGNAAGNTNTCTNANCNNTNAAANTCNTNGGAAAAGGTAANNCATAATCACCANGANGATTNCTNCNTNNNCAAGAAANTGNTGTTTCTGCNAATTCNTGAAACCCTNNNNNNNNAGATTCAATGTGTCATCTTTNNNNNNNNNNNNNNNNNTCTNNACNNNNNCTNNNNNNNNNNNNNNNTTNTGGGCAGTTTCNTGGCNCTATAGGGTNGANAATNAACNNTNNGATTAANAAGTGAANNGTCCATNNTNAAGTNACTAGNGNNNTTTNGTNNCANTTAANTGAGTTGTTCTTCNTCTTNAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTTNAATTNATTACTTGAATTTTAAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNANATGAANANATTGTTATTNNNANNAAAAAANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCCAAAAGTGATGTCTTCCCTNTTGGTTNTGACTCACAAATGGTTNAATNTCANNATTCTCTCCAAAATTATCGATNNTTTTTCAATCTCGTACACATTCATTAGTATATGTANACGATTCTTTTAATTAACTGAATTTTCATATTAGCTCCACGGTAAGTAGAGTGTCACCAACATCTCTTACACNTCATGTCCCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17284571_17284609_kn-0_can-0_99802_484_TRUE
SEQUENCE_TARGET=950,138
SEQUENCE_TEMPLATE=TGCTGGCTCGCTTATCATCTCCGCTGGGTTCATGACACTGGCAGTTCCAAACAGCCAAGGCTGTCTNAATTTTGGCTGNTTATCTTCTTTGCCTTTNGGTTGTTGGNANGTATGATCNANAGTTTCGATTGGTTTNNCAGANGGCTTGTCNNTNTCCACNAAACTAGGTTCAGGTGGNTCCACGGCTCTAGGAGTTGTTGAGCGGGTTGTGGAGATTGTGGTTGTGGGAAATGCTTTCTCTGGTGGCACAGCTGGTGTGTANTTAGATGGAAGTAGATCGTCCCAATTCTCTAGCAGTTCTTTATAGACTTTGCGTAGAGTTACCTCAGTTAGTCCGGTTATTTTACATATCTCTGCTTGTGTCTTTCGCTTGTCTTCAAGTTGACATGCCAGATAGATAGCAGCTGCAGAAATGCTGATCGGNTTTCTACGGGTGCAGAAGCATTTGTTGATCACAACTTCTCCAATNTGTGTTGCTAGTTCCTAGGAAGATTNAAAACNNACAAATACTTAGGGGGATCATAATAGGCATCAGAGTAGTGCATCNGAGATNTTGCAAAGTGTCACATTGGATNNNNNNAAGTCATTGGAAGCGGGAAAAAGTCCATGCATTGATACACTATTNACGTTGCATTTTAGGCCTTTCAAAAAGAAGNATAAGTNTAGTAGCACACCTGAGCAGATTTGTTTAGTTGAAGGAGGGTACAGAATCTTGGCATATGAACTGATATAGAGTTACTGTTGATGGGTTGACTTAGTTGTAGAGCTTCTCCTAAAATCTTGATGTACTTCCCAATTTCCTTCTGCTGTACATTGGCTGCAATGGAGATTTCCTATTGTGCAAAGNAAGACAAATACATTAGACACAAATCAAGTCTCAATAATAAACTGAAAGAAGTGCTGCTCCTGTATAAGTTCATAAGTGATGGTCCACATTTGGTTGTAACAAAATCTGCCTCTAGAAAAGATAATTCTGTATTCTACGATANGATATCCATNTNAAAGCTAATTTACGAACAAAACATGTCAAAAATATCANCAAACAACNGAGACAAATGATAAGAACAGTTATNNNNNNNNNTAAAGTACACCAACATGGAAGCTTNATTCCTTCAAGNAAACACACAAGCTTNAGAAACCTAGNATAGACTGGTNNTANTTCTTCAATTCGAAAACNNNGNNAGCNGTTCAAGAAANTACANTGATNANTTATCCAGAATNAACCANATAGTCACAACTTCTTTCAACNCAAAACGATGAGAACGAANTNGNTTTNTAACATAAACNNNNCATCTAGCAATGTAACTTCAANNNATTGAGNCTNANNAGCTNAATCTGCCATTANAACTTGTAATGANATAGGTTCTCTAATTACCTGAAGAGTTCTAGGTTCTTGAGCCTCCCTAATAGCTTGAACAAGGCAAGCAGTGGCTAAAGCTTCAACGCTTCTGTTTCGCAAGCAAGTAGCTGAGCAACAATCCCTAAACAACTGGAAAGCATGCTCAGAGATATCGCAATCCAANCCTAAAATCGANGCNACATCGATAATCTGCATATAAGCCCTAAGATTATCCACCACAACAGTCGACGAATTCGAATTCGATGTTGACGAAGCGAGTTCTAGGGTTCGTTCGAGTTCAGCGAGATGACCGGAGAAAGAGAGGGAAGAGCGAGCGAANATNGGGCTTGGCTCGAGNGACCAGGTAGAGAAGGCGGTNATGAATCCGGTNGGNTCNAACGGATCTTCCTCGTCTTCAGGAGACGGTTGCGCCGCNNTTTGGAGATCNGAAGTNACNANNCAGAGNGGAGTGTCTTGGGCNCGGAGATGNAANAGGTGGTGGTTTTGCGTCTGNCGTTCTTCCATTACGCGACCGCAAGATGAGCACTCCGTTATTGATCTACCGGAGCTNGTGGTGGNACACCGTCCCTGCGCCGATGAACAGTACGGACACTTCATTTTTGNGTTTCTTTACNTTTNNNTTTTTTTTTTNTNNNCTCTCANGTGNGAGNGTGNTAAAGCTNTTTTATACTACAAGAG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.413143
PRIMER_LEFT_0_PENALTY=0.216240
PRIMER_RIGHT_0_PENALTY=0.196903
PRIMER_LEFT_0_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_0_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_0=923,20
PRIMER_RIGHT_0=1756,20
PRIMER_LEFT_0_TM=60.216
PRIMER_RIGHT_0_TM=59.803
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=834
PRIMER_PAIR_1_PENALTY=0.449463
PRIMER_LEFT_1_PENALTY=0.216240
PRIMER_RIGHT_1_PENALTY=0.233224
PRIMER_LEFT_1_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_1_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_1=923,20
PRIMER_RIGHT_1=1674,20
PRIMER_LEFT_1_TM=60.216
PRIMER_RIGHT_1_TM=60.233
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=752
PRIMER_PAIR_2_PENALTY=0.526453
PRIMER_LEFT_2_PENALTY=0.329550
PRIMER_RIGHT_2_PENALTY=0.196903
PRIMER_LEFT_2_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_2_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_2=790,20
PRIMER_RIGHT_2=1756,20
PRIMER_LEFT_2_TM=59.670
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=967
PRIMER_PAIR_3_PENALTY=0.556459
PRIMER_LEFT_3_PENALTY=0.216240
PRIMER_RIGHT_3_PENALTY=0.340219
PRIMER_LEFT_3_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_3_SEQUENCE=GCGTTGAAGCTTTAGCCACT
PRIMER_LEFT_3=923,20
PRIMER_RIGHT_3=1445,20
PRIMER_LEFT_3_TM=60.216
PRIMER_RIGHT_3_TM=59.660
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=523
PRIMER_PAIR_4_PENALTY=0.561958
PRIMER_LEFT_4_PENALTY=0.216240
PRIMER_RIGHT_4_PENALTY=0.345718
PRIMER_LEFT_4_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_4_SEQUENCE=CTTTAGCCACTGCTTGCCTT
PRIMER_LEFT_4=923,20
PRIMER_RIGHT_4=1436,20
PRIMER_LEFT_4_TM=60.216
PRIMER_RIGHT_4_TM=59.654
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=514
PRIMER_PAIR_5_PENALTY=0.562774
PRIMER_LEFT_5_PENALTY=0.329550
PRIMER_RIGHT_5_PENALTY=0.233224
PRIMER_LEFT_5_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_5_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_5=790,20
PRIMER_RIGHT_5=1674,20
PRIMER_LEFT_5_TM=59.670
PRIMER_RIGHT_5_TM=60.233
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=885
PRIMER_PAIR_6_PENALTY=0.619510
PRIMER_LEFT_6_PENALTY=0.216240
PRIMER_RIGHT_6_PENALTY=0.403270
PRIMER_LEFT_6_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_6_SEQUENCE=ATTCGTCGACTGTTGTGGTG
PRIMER_LEFT_6=923,20
PRIMER_RIGHT_6=1592,20
PRIMER_LEFT_6_TM=60.216
PRIMER_RIGHT_6_TM=59.597
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=670
PRIMER_PAIR_7_PENALTY=0.619815
PRIMER_LEFT_7_PENALTY=0.279595
PRIMER_RIGHT_7_PENALTY=0.340219
PRIMER_LEFT_7_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_7_SEQUENCE=GCGTTGAAGCTTTAGCCACT
PRIMER_LEFT_7=626,20
PRIMER_RIGHT_7=1445,20
PRIMER_LEFT_7_TM=59.720
PRIMER_RIGHT_7_TM=59.660
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=820
PRIMER_PAIR_8_PENALTY=0.625313
PRIMER_LEFT_8_PENALTY=0.279595
PRIMER_RIGHT_8_PENALTY=0.345718
PRIMER_LEFT_8_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_8_SEQUENCE=CTTTAGCCACTGCTTGCCTT
PRIMER_LEFT_8=626,20
PRIMER_RIGHT_8=1436,20
PRIMER_LEFT_8_TM=59.720
PRIMER_RIGHT_8_TM=59.654
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=811
PRIMER_PAIR_9_PENALTY=0.669769
PRIMER_LEFT_9_PENALTY=0.329550
PRIMER_RIGHT_9_PENALTY=0.340219
PRIMER_LEFT_9_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_9_SEQUENCE=GCGTTGAAGCTTTAGCCACT
PRIMER_LEFT_9=790,20
PRIMER_RIGHT_9=1445,20
PRIMER_LEFT_9_TM=59.670
PRIMER_RIGHT_9_TM=59.660
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=656
PRIMER_PAIR_10_PENALTY=0.675268
PRIMER_LEFT_10_PENALTY=0.329550
PRIMER_RIGHT_10_PENALTY=0.345718
PRIMER_LEFT_10_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_10_SEQUENCE=CTTTAGCCACTGCTTGCCTT
PRIMER_LEFT_10=790,20
PRIMER_RIGHT_10=1436,20
PRIMER_LEFT_10_TM=59.670
PRIMER_RIGHT_10_TM=59.654
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=647
PRIMER_PAIR_11_PENALTY=0.682866
PRIMER_LEFT_11_PENALTY=0.279595
PRIMER_RIGHT_11_PENALTY=0.403270
PRIMER_LEFT_11_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_11_SEQUENCE=ATTCGTCGACTGTTGTGGTG
PRIMER_LEFT_11=626,20
PRIMER_RIGHT_11=1592,20
PRIMER_LEFT_11_TM=59.720
PRIMER_RIGHT_11_TM=59.597
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=967
PRIMER_PAIR_12_PENALTY=0.732820
PRIMER_LEFT_12_PENALTY=0.329550
PRIMER_RIGHT_12_PENALTY=0.403270
PRIMER_LEFT_12_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_12_SEQUENCE=ATTCGTCGACTGTTGTGGTG
PRIMER_LEFT_12=790,20
PRIMER_RIGHT_12=1592,20
PRIMER_LEFT_12_TM=59.670
PRIMER_RIGHT_12_TM=59.597
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=803
PRIMER_PAIR_13_PENALTY=0.745100
PRIMER_LEFT_13_PENALTY=0.216240
PRIMER_RIGHT_13_PENALTY=0.528861
PRIMER_LEFT_13_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_13_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_13=923,20
PRIMER_RIGHT_13=1625,20
PRIMER_LEFT_13_TM=60.216
PRIMER_RIGHT_13_TM=60.529
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=703
PRIMER_PAIR_14_PENALTY=0.771318
PRIMER_LEFT_14_PENALTY=0.431098
PRIMER_RIGHT_14_PENALTY=0.340219
PRIMER_LEFT_14_SEQUENCE=ATTGGAAGCGGGAAAAAGTC
PRIMER_RIGHT_14_SEQUENCE=GCGTTGAAGCTTTAGCCACT
PRIMER_LEFT_14=585,20
PRIMER_RIGHT_14=1445,20
PRIMER_LEFT_14_TM=60.431
PRIMER_RIGHT_14_TM=59.660
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=861
PRIMER_PAIR_15_PENALTY=0.808456
PRIMER_LEFT_15_PENALTY=0.279595
PRIMER_RIGHT_15_PENALTY=0.528861
PRIMER_LEFT_15_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_15_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_15=626,20
PRIMER_RIGHT_15=1625,20
PRIMER_LEFT_15_TM=59.720
PRIMER_RIGHT_15_TM=60.529
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=1000
PRIMER_PAIR_16_PENALTY=0.858410
PRIMER_LEFT_16_PENALTY=0.329550
PRIMER_RIGHT_16_PENALTY=0.528861
PRIMER_LEFT_16_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_16_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_16=790,20
PRIMER_RIGHT_16=1625,20
PRIMER_LEFT_16_TM=59.670
PRIMER_RIGHT_16_TM=60.529
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=836
PRIMER_PAIR_17_PENALTY=0.883159
PRIMER_LEFT_17_PENALTY=0.686256
PRIMER_RIGHT_17_PENALTY=0.196903
PRIMER_LEFT_17_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_17_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_17=889,20
PRIMER_RIGHT_17=1756,20
PRIMER_LEFT_17_TM=59.314
PRIMER_RIGHT_17_TM=59.803
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=868
PRIMER_PAIR_18_PENALTY=0.919480
PRIMER_LEFT_18_PENALTY=0.686256
PRIMER_RIGHT_18_PENALTY=0.233224
PRIMER_LEFT_18_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_18_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_18=889,20
PRIMER_RIGHT_18=1674,20
PRIMER_LEFT_18_TM=59.314
PRIMER_RIGHT_18_TM=60.233
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=786
PRIMER_PAIR_19_PENALTY=0.920600
PRIMER_LEFT_19_PENALTY=0.216240
PRIMER_RIGHT_19_PENALTY=0.704360
PRIMER_LEFT_19_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_19_SEQUENCE=TTCCCTCTCTTTCTCCGGTC
PRIMER_LEFT_19=923,20
PRIMER_RIGHT_19=1666,20
PRIMER_LEFT_19_TM=60.216
PRIMER_RIGHT_19_TM=60.704
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=744
PRIMER_PAIR_20_PENALTY=1.026475
PRIMER_LEFT_20_PENALTY=0.686256
PRIMER_RIGHT_20_PENALTY=0.340219
PRIMER_LEFT_20_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_20_SEQUENCE=GCGTTGAAGCTTTAGCCACT
PRIMER_LEFT_20=889,20
PRIMER_RIGHT_20=1445,20
PRIMER_LEFT_20_TM=59.314
PRIMER_RIGHT_20_TM=59.660
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=557
PRIMER_PAIR_21_PENALTY=1.031974
PRIMER_LEFT_21_PENALTY=0.686256
PRIMER_RIGHT_21_PENALTY=0.345718
PRIMER_LEFT_21_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_21_SEQUENCE=CTTTAGCCACTGCTTGCCTT
PRIMER_LEFT_21=889,20
PRIMER_RIGHT_21=1436,20
PRIMER_LEFT_21_TM=59.314
PRIMER_RIGHT_21_TM=59.654
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=548
PRIMER_PAIR_22_PENALTY=1.033910
PRIMER_LEFT_22_PENALTY=0.329550
PRIMER_RIGHT_22_PENALTY=0.704360
PRIMER_LEFT_22_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_22_SEQUENCE=TTCCCTCTCTTTCTCCGGTC
PRIMER_LEFT_22=790,20
PRIMER_RIGHT_22=1666,20
PRIMER_LEFT_22_TM=59.670
PRIMER_RIGHT_22_TM=60.704
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=9.6000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=877
PRIMER_PAIR_23_PENALTY=1.077403
PRIMER_LEFT_23_PENALTY=0.216240
PRIMER_RIGHT_23_PENALTY=0.861164
PRIMER_LEFT_23_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_23_SEQUENCE=GAATTCGTCGACTGTTGTGG
PRIMER_LEFT_23=923,20
PRIMER_RIGHT_23=1594,20
PRIMER_LEFT_23_TM=60.216
PRIMER_RIGHT_23_TM=59.139
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=672
PRIMER_PAIR_24_PENALTY=1.089526
PRIMER_LEFT_24_PENALTY=0.686256
PRIMER_RIGHT_24_PENALTY=0.403270
PRIMER_LEFT_24_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_24_SEQUENCE=ATTCGTCGACTGTTGTGGTG
PRIMER_LEFT_24=889,20
PRIMER_RIGHT_24=1592,20
PRIMER_LEFT_24_TM=59.314
PRIMER_RIGHT_24_TM=59.597
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=704
PRIMER_PAIR_25_PENALTY=1.099923
PRIMER_LEFT_25_PENALTY=0.216240
PRIMER_RIGHT_25_PENALTY=0.883683
PRIMER_LEFT_25_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_25_SEQUENCE=TCATCTCGCTGAACTCGAAC
PRIMER_LEFT_25=923,20
PRIMER_RIGHT_25=1648,20
PRIMER_LEFT_25_TM=60.216
PRIMER_RIGHT_25_TM=59.116
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=726
PRIMER_PAIR_26_PENALTY=1.140759
PRIMER_LEFT_26_PENALTY=0.279595
PRIMER_RIGHT_26_PENALTY=0.861164
PRIMER_LEFT_26_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_26_SEQUENCE=GAATTCGTCGACTGTTGTGG
PRIMER_LEFT_26=626,20
PRIMER_RIGHT_26=1594,20
PRIMER_LEFT_26_TM=59.720
PRIMER_RIGHT_26_TM=59.139
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=969
PRIMER_PAIR_27_PENALTY=1.179948
PRIMER_LEFT_27_PENALTY=0.216240
PRIMER_RIGHT_27_PENALTY=0.963709
PRIMER_LEFT_27_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_27_SEQUENCE=TAGCCACTGCTTGCCTTGTT
PRIMER_LEFT_27=923,20
PRIMER_RIGHT_27=1433,20
PRIMER_LEFT_27_TM=60.216
PRIMER_RIGHT_27_TM=60.964
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=511
PRIMER_PAIR_28_PENALTY=1.179948
PRIMER_LEFT_28_PENALTY=0.216240
PRIMER_RIGHT_28_PENALTY=0.963709
PRIMER_LEFT_28_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_28_SEQUENCE=TTAGCCACTGCTTGCCTTGT
PRIMER_LEFT_28=923,20
PRIMER_RIGHT_28=1434,20
PRIMER_LEFT_28_TM=60.216
PRIMER_RIGHT_28_TM=60.964
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=512
PRIMER_PAIR_29_PENALTY=1.190713
PRIMER_LEFT_29_PENALTY=0.329550
PRIMER_RIGHT_29_PENALTY=0.861164
PRIMER_LEFT_29_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_29_SEQUENCE=GAATTCGTCGACTGTTGTGG
PRIMER_LEFT_29=790,20
PRIMER_RIGHT_29=1594,20
PRIMER_LEFT_29_TM=59.670
PRIMER_RIGHT_29_TM=59.139
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=805
PRIMER_PAIR_30_PENALTY=1.213233
PRIMER_LEFT_30_PENALTY=0.329550
PRIMER_RIGHT_30_PENALTY=0.883683
PRIMER_LEFT_30_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_30_SEQUENCE=TCATCTCGCTGAACTCGAAC
PRIMER_LEFT_30=790,20
PRIMER_RIGHT_30=1648,20
PRIMER_LEFT_30_TM=59.670
PRIMER_RIGHT_30_TM=59.116
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=859
PRIMER_PAIR_31_PENALTY=1.215116
PRIMER_LEFT_31_PENALTY=0.686256
PRIMER_RIGHT_31_PENALTY=0.528861
PRIMER_LEFT_31_SEQUENCE=TGAAAGAAGTGCTGCTCCTG
PRIMER_RIGHT_31_SEQUENCE=CCCTAGAACTCGCTTCGTCA
PRIMER_LEFT_31=889,20
PRIMER_RIGHT_31=1625,20
PRIMER_LEFT_31_TM=59.314
PRIMER_RIGHT_31_TM=60.529
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=737
PRIMER_PAIR_32_PENALTY=1.228129
PRIMER_LEFT_32_PENALTY=0.216240
PRIMER_RIGHT_32_PENALTY=1.011889
PRIMER_LEFT_32_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_32_SEQUENCE=GTCATCTCGCTGAACTCGAAC
PRIMER_LEFT_32=923,20
PRIMER_RIGHT_32=1649,21
PRIMER_LEFT_32_TM=60.216
PRIMER_RIGHT_32_TM=60.012
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=727
PRIMER_PAIR_33_PENALTY=1.243304
PRIMER_LEFT_33_PENALTY=0.279595
PRIMER_RIGHT_33_PENALTY=0.963709
PRIMER_LEFT_33_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_33_SEQUENCE=TAGCCACTGCTTGCCTTGTT
PRIMER_LEFT_33=626,20
PRIMER_RIGHT_33=1433,20
PRIMER_LEFT_33_TM=59.720
PRIMER_RIGHT_33_TM=60.964
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=808
PRIMER_PAIR_34_PENALTY=1.243304
PRIMER_LEFT_34_PENALTY=0.279595
PRIMER_RIGHT_34_PENALTY=0.963709
PRIMER_LEFT_34_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_34_SEQUENCE=TTAGCCACTGCTTGCCTTGT
PRIMER_LEFT_34=626,20
PRIMER_RIGHT_34=1434,20
PRIMER_LEFT_34_TM=59.720
PRIMER_RIGHT_34_TM=60.964
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=809
PRIMER_PAIR_35_PENALTY=1.243832
PRIMER_LEFT_35_PENALTY=0.216240
PRIMER_RIGHT_35_PENALTY=1.027592
PRIMER_LEFT_35_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_35_SEQUENCE=GAATTCGTCGACTGTTGTGGT
PRIMER_LEFT_35=923,20
PRIMER_RIGHT_35=1594,21
PRIMER_LEFT_35_TM=60.216
PRIMER_RIGHT_35_TM=60.028
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=672
PRIMER_PAIR_36_PENALTY=1.293258
PRIMER_LEFT_36_PENALTY=0.329550
PRIMER_RIGHT_36_PENALTY=0.963709
PRIMER_LEFT_36_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_36_SEQUENCE=TAGCCACTGCTTGCCTTGTT
PRIMER_LEFT_36=790,20
PRIMER_RIGHT_36=1433,20
PRIMER_LEFT_36_TM=59.670
PRIMER_RIGHT_36_TM=60.964
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=644
PRIMER_PAIR_37_PENALTY=1.293258
PRIMER_LEFT_37_PENALTY=0.329550
PRIMER_RIGHT_37_PENALTY=0.963709
PRIMER_LEFT_37_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_37_SEQUENCE=TTAGCCACTGCTTGCCTTGT
PRIMER_LEFT_37=790,20
PRIMER_RIGHT_37=1434,20
PRIMER_LEFT_37_TM=59.670
PRIMER_RIGHT_37_TM=60.964
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=645
PRIMER_PAIR_38_PENALTY=1.296058
PRIMER_LEFT_38_PENALTY=0.216240
PRIMER_RIGHT_38_PENALTY=1.079819
PRIMER_LEFT_38_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_38_SEQUENCE=GCGAAACAGAAGCGTTGAAG
PRIMER_LEFT_38=923,20
PRIMER_RIGHT_38=1456,20
PRIMER_LEFT_38_TM=60.216
PRIMER_RIGHT_38_TM=61.080
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=534
PRIMER_PAIR_39_PENALTY=1.307002
PRIMER_LEFT_39_PENALTY=1.110099
PRIMER_RIGHT_39_PENALTY=0.196903
PRIMER_LEFT_39_SEQUENCE=TCCTTCTGCTGTACATTGGCT
PRIMER_RIGHT_39_SEQUENCE=TGAAGACGAGGAAGATCCGT
PRIMER_LEFT_39=798,21
PRIMER_RIGHT_39=1756,20
PRIMER_LEFT_39_TM=59.890
PRIMER_RIGHT_39_TM=59.803
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=959
PRIMER_PAIR_40_PENALTY=1.307188
PRIMER_LEFT_40_PENALTY=0.279595
PRIMER_RIGHT_40_PENALTY=1.027592
PRIMER_LEFT_40_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_40_SEQUENCE=GAATTCGTCGACTGTTGTGGT
PRIMER_LEFT_40=626,20
PRIMER_RIGHT_40=1594,21
PRIMER_LEFT_40_TM=59.720
PRIMER_RIGHT_40_TM=60.028
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=969
PRIMER_PAIR_41_PENALTY=1.316833
PRIMER_LEFT_41_PENALTY=0.216240
PRIMER_RIGHT_41_PENALTY=1.100593
PRIMER_LEFT_41_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_41_SEQUENCE=AACCCTAGAACTCGCTTCGTC
PRIMER_LEFT_41=923,20
PRIMER_RIGHT_41=1627,21
PRIMER_LEFT_41_TM=60.216
PRIMER_RIGHT_41_TM=59.899
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=705
PRIMER_PAIR_42_PENALTY=1.316833
PRIMER_LEFT_42_PENALTY=0.216240
PRIMER_RIGHT_42_PENALTY=1.100593
PRIMER_LEFT_42_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_42_SEQUENCE=GAACCCTAGAACTCGCTTCGT
PRIMER_LEFT_42=923,20
PRIMER_RIGHT_42=1628,21
PRIMER_LEFT_42_TM=60.216
PRIMER_RIGHT_42_TM=59.899
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=706
PRIMER_PAIR_43_PENALTY=1.318485
PRIMER_LEFT_43_PENALTY=0.152999
PRIMER_RIGHT_43_PENALTY=1.165486
PRIMER_LEFT_43_SEQUENCE=GGTGCAGAAGCATTTGTTGA
PRIMER_RIGHT_43_SEQUENCE=GGGAGGCTCAAGAACCTAGAA
PRIMER_LEFT_43=432,20
PRIMER_RIGHT_43=1403,21
PRIMER_LEFT_43_TM=59.847
PRIMER_RIGHT_43_TM=59.835
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.0000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=972
PRIMER_PAIR_44_PENALTY=1.332205
PRIMER_LEFT_44_PENALTY=0.216240
PRIMER_RIGHT_44_PENALTY=1.115965
PRIMER_LEFT_44_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_44_SEQUENCE=CTCGCTGAACTCGAACGAAC
PRIMER_LEFT_44=923,20
PRIMER_RIGHT_44=1644,20
PRIMER_LEFT_44_TM=60.216
PRIMER_RIGHT_44_TM=61.116
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=722
PRIMER_PAIR_45_PENALTY=1.341439
PRIMER_LEFT_45_PENALTY=0.329550
PRIMER_RIGHT_45_PENALTY=1.011889
PRIMER_LEFT_45_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_45_SEQUENCE=GTCATCTCGCTGAACTCGAAC
PRIMER_LEFT_45=790,20
PRIMER_RIGHT_45=1649,21
PRIMER_LEFT_45_TM=59.670
PRIMER_RIGHT_45_TM=60.012
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=860
PRIMER_PAIR_46_PENALTY=1.343323
PRIMER_LEFT_46_PENALTY=1.110099
PRIMER_RIGHT_46_PENALTY=0.233224
PRIMER_LEFT_46_SEQUENCE=TCCTTCTGCTGTACATTGGCT
PRIMER_RIGHT_46_SEQUENCE=GCTCGCTCTTCCCTCTCTTT
PRIMER_LEFT_46=798,21
PRIMER_RIGHT_46=1674,20
PRIMER_LEFT_46_TM=59.890
PRIMER_RIGHT_46_TM=60.233
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=877
PRIMER_PAIR_47_PENALTY=1.357142
PRIMER_LEFT_47_PENALTY=0.329550
PRIMER_RIGHT_47_PENALTY=1.027592
PRIMER_LEFT_47_SEQUENCE=TCCCAATTTCCTTCTGCTGT
PRIMER_RIGHT_47_SEQUENCE=GAATTCGTCGACTGTTGTGGT
PRIMER_LEFT_47=790,20
PRIMER_RIGHT_47=1594,21
PRIMER_LEFT_47_TM=59.670
PRIMER_RIGHT_47_TM=60.028
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=7.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=805
PRIMER_PAIR_48_PENALTY=1.359414
PRIMER_LEFT_48_PENALTY=0.279595
PRIMER_RIGHT_48_PENALTY=1.079819
PRIMER_LEFT_48_SEQUENCE=CGTTGCATTTTAGGCCTTTC
PRIMER_RIGHT_48_SEQUENCE=GCGAAACAGAAGCGTTGAAG
PRIMER_LEFT_48=626,20
PRIMER_RIGHT_48=1456,20
PRIMER_LEFT_48_TM=59.720
PRIMER_RIGHT_48_TM=61.080
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=831
PRIMER_PAIR_49_PENALTY=1.381726
PRIMER_LEFT_49_PENALTY=0.216240
PRIMER_RIGHT_49_PENALTY=1.165486
PRIMER_LEFT_49_SEQUENCE=TGATGGTCCACATTTGGTTG
PRIMER_RIGHT_49_SEQUENCE=GGGAGGCTCAAGAACCTAGAA
PRIMER_LEFT_49=923,20
PRIMER_RIGHT_49=1403,21
PRIMER_LEFT_49_TM=60.216
PRIMER_RIGHT_49_TM=59.835
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=481
=
SEQUENCE_ID=MAGIC.149_4_3145244_3145375_edi-0_wil-2_327930_125575_TRUE
SEQUENCE_TARGET=950,231
SEQUENCE_TEMPLATE=CAANTNCTTTNTAGAATCNAANAGGAAATTNNANACTGTNATTTGCGATAAAGATGAGTGAGAGAAACTNGTTCTTAGATTTNCTTTTATAACGAGAAGTTTCCTTTGGAATCAGAGCAGTTGCATCACGAAGTGGGGANATCCACGACATGGAGATAACCGTCGCNNNNNNNNNNNNNANGAACTGCCCNCATCNTCGACAAAACCGTCNGAGATCNACTCCNGGTAACCGTTANAGCAGATCCAAAANTAANTGTGTATCGCATNCACGATCGCCTTTGCAATCGNNNCCATTNCNTTNNNNNNNATNTGATCCTATGAGATTTNTTTTTTTATATAAAATGATACAAAAGATTAGATTTGAATCTAAATAAACATTTTTCATAAAAACATTTGAGTATNANAANNNCNTCAGCTTTTNNTCTACTGTAAAAAGCTTCTAAAACATTCTCANCCTTCTCCATATTTTNNAGTTCNATGATGATGTATCTTTGTGGAAGANAAACCACCGAACNAGTAATCTNGACGTTAGTACANCTNTNTACCCACAAAGGAGNGAATNCAAAATAGACTACTTTATACTCACNTCNAGANTTAAGATTTGATTGNTNTTCTACTCACACCAAANCAAAAACCAAAGATCATAAAGATCAGATTATTCGTCCTTTNNTCGAANCTTATANATTTATTATTTTATTGANCANTATCTGAAATAANAACAACATNATCANATATTTAACNANAAAAGGTTAATAATAAGGATTNNNNCTGGGGACAAANCCAATCATNCTTGAACGCCATTTCATATGTGTTCTCATTNAAGTANAAANTCGTTACCAATGANAACAAATTTAGCAATGAAACTCATATGGNTGTAATNACNANAAGCAAAATTAATTAAAAACACTTTACCTTCCAAATACAAATAACTCNGAGAGGATCCNCTACCAAGTTCTTCATTGTTTGTTGAAAGAGCNTTTATTGACTCCNNTGGGACACNNTGATGTATNTGTGCNTGGAATGTGTTGAAACATTTTTNNAGAACCTGCAACAAAATTAATATTAGTGATGNTANCTAATTNGCAATAGATAGAANCTAATTCTAGTANAGAATCTTTACTTGAATTCCNNNAACCGATTNTCTTANACANAATCCTTANANAAGTTGTAGAGAATGAAAGATATGCAAGCNTTCTGTTCGAAACATCGAGAATGANCTCATNAGAAAACCCANNCGGTCAAACAGATGCCTGCAANTATTTTACAATCAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGAAANTATTNTGTTTCNAAAGTTAATAATATTTTCTNTTGNNACATTTTGCTATGTTTAGGCTATAACACTNTGAAGGNAGATTNTTGAGAAAGGCGGGTTTACGCAGTTTAGACCTCATTATATTACTTGGTATTGTCCTCATACTATCACGTTGAGTCGACAGTNTAAGTCTCANTGTATCAATNAAGGAAGGTATTGTCCTCCTGATCTAGAGCANGACTTTAGCTCGGGATACGATGGAAAAGACGTGGTTATGGAAAATTTGAGACAGCTTTGTGTTTACAAGGTGGCGAATGCAACCGCCACCTTGGGTGTAGTGGNATTATGTTACTGATTTCCAGATCAGATATCCCATGAAGGAGNAAAAAATACAATAAGGAGTGTGCTGAGTCTGTAATCAAATCTCTTGGTGAGTGTTTCTTCAAACATGTCTTGATAGTTAAAAGAAAAGCAATTTTTGGGAGAATGAGGTATTGAGGTCATATGGGAGACCCTAATGTTGACTTGGACAATAATNCAGCTTTAAAGGAAGAACAAGATGCTCAAGTAAATTACTCTTCTCACATCTGAATTTGACTCTTCTTTACGTAACCTGTTTTGGGTTTGACATATTATATTATGTAGGTTGGCAAGGGTANAAGGGGTGATGTTACCATATTGCCTACCTTAGTTGTCAACAANAGACAGTACCGAGGTTTGTTCCGTAGTATTCAACTTGAGACTTCAATATATGTAGTTTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.946454
PRIMER_LEFT_0_PENALTY=2.893725
PRIMER_RIGHT_0_PENALTY=0.052729
PRIMER_LEFT_0_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_0_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_0=795,21
PRIMER_RIGHT_0=1630,20
PRIMER_LEFT_0_TM=58.106
PRIMER_RIGHT_0_TM=60.053
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=836
PRIMER_PAIR_1_PENALTY=3.073967
PRIMER_LEFT_1_PENALTY=3.021238
PRIMER_RIGHT_1_PENALTY=0.052729
PRIMER_LEFT_1_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_1_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_1=794,22
PRIMER_RIGHT_1=1630,20
PRIMER_LEFT_1_TM=58.979
PRIMER_RIGHT_1_TM=60.053
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=837
PRIMER_PAIR_2_PENALTY=3.440136
PRIMER_LEFT_2_PENALTY=2.893725
PRIMER_RIGHT_2_PENALTY=0.546410
PRIMER_LEFT_2_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_2_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_2=795,21
PRIMER_RIGHT_2=1643,20
PRIMER_LEFT_2_TM=58.106
PRIMER_RIGHT_2_TM=59.454
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=849
PRIMER_PAIR_3_PENALTY=3.506944
PRIMER_LEFT_3_PENALTY=2.893725
PRIMER_RIGHT_3_PENALTY=0.613219
PRIMER_LEFT_3_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_3_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_3=795,21
PRIMER_RIGHT_3=1638,20
PRIMER_LEFT_3_TM=58.106
PRIMER_RIGHT_3_TM=59.387
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=844
PRIMER_PAIR_4_PENALTY=3.567649
PRIMER_LEFT_4_PENALTY=3.021238
PRIMER_RIGHT_4_PENALTY=0.546410
PRIMER_LEFT_4_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_4_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_4=794,22
PRIMER_RIGHT_4=1643,20
PRIMER_LEFT_4_TM=58.979
PRIMER_RIGHT_4_TM=59.454
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=850
PRIMER_PAIR_5_PENALTY=3.634457
PRIMER_LEFT_5_PENALTY=3.021238
PRIMER_RIGHT_5_PENALTY=0.613219
PRIMER_LEFT_5_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_5_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_5=794,22
PRIMER_RIGHT_5=1638,20
PRIMER_LEFT_5_TM=58.979
PRIMER_RIGHT_5_TM=59.387
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=9.3000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=845
PRIMER_PAIR_6_PENALTY=3.925545
PRIMER_LEFT_6_PENALTY=3.872816
PRIMER_RIGHT_6_PENALTY=0.052729
PRIMER_LEFT_6_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_6_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_6=795,20
PRIMER_RIGHT_6=1630,20
PRIMER_LEFT_6_TM=56.127
PRIMER_RIGHT_6_TM=60.053
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=836
PRIMER_PAIR_7_PENALTY=4.094803
PRIMER_LEFT_7_PENALTY=2.893725
PRIMER_RIGHT_7_PENALTY=1.201078
PRIMER_LEFT_7_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_7_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_7=795,21
PRIMER_RIGHT_7=1639,21
PRIMER_LEFT_7_TM=58.106
PRIMER_RIGHT_7_TM=60.201
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=9.3000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=845
PRIMER_PAIR_8_PENALTY=4.097174
PRIMER_LEFT_8_PENALTY=4.044445
PRIMER_RIGHT_8_PENALTY=0.052729
PRIMER_LEFT_8_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_8_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_8=794,20
PRIMER_RIGHT_8=1630,20
PRIMER_LEFT_8_TM=55.956
PRIMER_RIGHT_8_TM=60.053
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.3000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=837
PRIMER_PAIR_9_PENALTY=4.222316
PRIMER_LEFT_9_PENALTY=3.021238
PRIMER_RIGHT_9_PENALTY=1.201078
PRIMER_LEFT_9_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_9_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_9=794,22
PRIMER_RIGHT_9=1639,21
PRIMER_LEFT_9_TM=58.979
PRIMER_RIGHT_9_TM=60.201
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=9.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=846
PRIMER_PAIR_10_PENALTY=4.244156
PRIMER_LEFT_10_PENALTY=2.893725
PRIMER_RIGHT_10_PENALTY=1.350430
PRIMER_LEFT_10_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_10_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_10=795,21
PRIMER_RIGHT_10=1648,21
PRIMER_LEFT_10_TM=58.106
PRIMER_RIGHT_10_TM=60.350
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=854
PRIMER_PAIR_11_PENALTY=4.371669
PRIMER_LEFT_11_PENALTY=3.021238
PRIMER_RIGHT_11_PENALTY=1.350430
PRIMER_LEFT_11_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_11_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_11=794,22
PRIMER_RIGHT_11=1648,21
PRIMER_LEFT_11_TM=58.979
PRIMER_RIGHT_11_TM=60.350
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=855
PRIMER_PAIR_12_PENALTY=4.408927
PRIMER_LEFT_12_PENALTY=2.893725
PRIMER_RIGHT_12_PENALTY=1.515201
PRIMER_LEFT_12_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_12_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_12=795,21
PRIMER_RIGHT_12=1644,21
PRIMER_LEFT_12_TM=58.106
PRIMER_RIGHT_12_TM=59.485
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=850
PRIMER_PAIR_13_PENALTY=4.419194
PRIMER_LEFT_13_PENALTY=2.893725
PRIMER_RIGHT_13_PENALTY=1.525468
PRIMER_LEFT_13_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_13_SEQUENCE=CCATAACCACGTCTTTTCCA
PRIMER_LEFT_13=795,21
PRIMER_RIGHT_13=1647,20
PRIMER_LEFT_13_TM=58.106
PRIMER_RIGHT_13_TM=58.475
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=853
PRIMER_PAIR_14_PENALTY=4.419226
PRIMER_LEFT_14_PENALTY=3.872816
PRIMER_RIGHT_14_PENALTY=0.546410
PRIMER_LEFT_14_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_14_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_14=795,20
PRIMER_RIGHT_14=1643,20
PRIMER_LEFT_14_TM=56.127
PRIMER_RIGHT_14_TM=59.454
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=849
PRIMER_PAIR_15_PENALTY=4.450494
PRIMER_LEFT_15_PENALTY=2.893725
PRIMER_RIGHT_15_PENALTY=1.556768
PRIMER_LEFT_15_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_15_SEQUENCE=CCACGTCTTTTCCATCGTATC
PRIMER_LEFT_15=795,21
PRIMER_RIGHT_15=1641,21
PRIMER_LEFT_15_TM=58.106
PRIMER_RIGHT_15_TM=59.443
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=5.3000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=847
PRIMER_PAIR_16_PENALTY=4.486035
PRIMER_LEFT_16_PENALTY=3.872816
PRIMER_RIGHT_16_PENALTY=0.613219
PRIMER_LEFT_16_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_16_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_16=795,20
PRIMER_RIGHT_16=1638,20
PRIMER_LEFT_16_TM=56.127
PRIMER_RIGHT_16_TM=59.387
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=9.3000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=844
PRIMER_PAIR_17_PENALTY=4.536440
PRIMER_LEFT_17_PENALTY=3.021238
PRIMER_RIGHT_17_PENALTY=1.515201
PRIMER_LEFT_17_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_17_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_17=794,22
PRIMER_RIGHT_17=1644,21
PRIMER_LEFT_17_TM=58.979
PRIMER_RIGHT_17_TM=59.485
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=851
PRIMER_PAIR_18_PENALTY=4.546707
PRIMER_LEFT_18_PENALTY=3.021238
PRIMER_RIGHT_18_PENALTY=1.525468
PRIMER_LEFT_18_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_18_SEQUENCE=CCATAACCACGTCTTTTCCA
PRIMER_LEFT_18=794,22
PRIMER_RIGHT_18=1647,20
PRIMER_LEFT_18_TM=58.979
PRIMER_RIGHT_18_TM=58.475
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=854
PRIMER_PAIR_19_PENALTY=4.578007
PRIMER_LEFT_19_PENALTY=3.021238
PRIMER_RIGHT_19_PENALTY=1.556768
PRIMER_LEFT_19_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_19_SEQUENCE=CCACGTCTTTTCCATCGTATC
PRIMER_LEFT_19=794,22
PRIMER_RIGHT_19=1641,21
PRIMER_LEFT_19_TM=58.979
PRIMER_RIGHT_19_TM=59.443
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=5.3000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=848
PRIMER_PAIR_20_PENALTY=4.590856
PRIMER_LEFT_20_PENALTY=4.044445
PRIMER_RIGHT_20_PENALTY=0.546410
PRIMER_LEFT_20_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_20_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_20=794,20
PRIMER_RIGHT_20=1643,20
PRIMER_LEFT_20_TM=55.956
PRIMER_RIGHT_20_TM=59.454
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=8.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=850
PRIMER_PAIR_21_PENALTY=4.657664
PRIMER_LEFT_21_PENALTY=4.044445
PRIMER_RIGHT_21_PENALTY=0.613219
PRIMER_LEFT_21_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_21_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_21=794,20
PRIMER_RIGHT_21=1638,20
PRIMER_LEFT_21_TM=55.956
PRIMER_RIGHT_21_TM=59.387
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.3000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=845
PRIMER_PAIR_22_PENALTY=4.745773
PRIMER_LEFT_22_PENALTY=2.893725
PRIMER_RIGHT_22_PENALTY=1.852047
PRIMER_LEFT_22_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_22_SEQUENCE=TAAACTGCGTAAACCCGCCT
PRIMER_LEFT_22=795,21
PRIMER_RIGHT_22=1500,20
PRIMER_LEFT_22_TM=58.106
PRIMER_RIGHT_22_TM=61.852
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=11.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=706
PRIMER_PAIR_23_PENALTY=4.745773
PRIMER_LEFT_23_PENALTY=2.893725
PRIMER_RIGHT_23_PENALTY=1.852047
PRIMER_LEFT_23_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_23_SEQUENCE=CTAAACTGCGTAAACCCGCC
PRIMER_LEFT_23=795,21
PRIMER_RIGHT_23=1501,20
PRIMER_LEFT_23_TM=58.106
PRIMER_RIGHT_23_TM=61.852
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=12.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=707
PRIMER_PAIR_24_PENALTY=4.873286
PRIMER_LEFT_24_PENALTY=3.021238
PRIMER_RIGHT_24_PENALTY=1.852047
PRIMER_LEFT_24_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_24_SEQUENCE=TAAACTGCGTAAACCCGCCT
PRIMER_LEFT_24=794,22
PRIMER_RIGHT_24=1500,20
PRIMER_LEFT_24_TM=58.979
PRIMER_RIGHT_24_TM=61.852
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=11.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=707
PRIMER_PAIR_25_PENALTY=4.873286
PRIMER_LEFT_25_PENALTY=3.021238
PRIMER_RIGHT_25_PENALTY=1.852047
PRIMER_LEFT_25_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_25_SEQUENCE=CTAAACTGCGTAAACCCGCC
PRIMER_LEFT_25=794,22
PRIMER_RIGHT_25=1501,20
PRIMER_LEFT_25_TM=58.979
PRIMER_RIGHT_25_TM=61.852
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=12.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=708
PRIMER_PAIR_26_PENALTY=4.955357
PRIMER_LEFT_26_PENALTY=2.893725
PRIMER_RIGHT_26_PENALTY=2.061632
PRIMER_LEFT_26_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_26_SEQUENCE=TTCCATAACCACGTCTTTTCC
PRIMER_LEFT_26=795,21
PRIMER_RIGHT_26=1649,21
PRIMER_LEFT_26_TM=58.106
PRIMER_RIGHT_26_TM=58.938
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=855
PRIMER_PAIR_27_PENALTY=5.073893
PRIMER_LEFT_27_PENALTY=3.872816
PRIMER_RIGHT_27_PENALTY=1.201078
PRIMER_LEFT_27_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_27_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_27=795,20
PRIMER_RIGHT_27=1639,21
PRIMER_LEFT_27_TM=56.127
PRIMER_RIGHT_27_TM=60.201
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=9.3000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=845
PRIMER_PAIR_28_PENALTY=5.082870
PRIMER_LEFT_28_PENALTY=3.021238
PRIMER_RIGHT_28_PENALTY=2.061632
PRIMER_LEFT_28_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_28_SEQUENCE=TTCCATAACCACGTCTTTTCC
PRIMER_LEFT_28=794,22
PRIMER_RIGHT_28=1649,21
PRIMER_LEFT_28_TM=58.979
PRIMER_RIGHT_28_TM=58.938
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=856
PRIMER_PAIR_29_PENALTY=5.085291
PRIMER_LEFT_29_PENALTY=2.893725
PRIMER_RIGHT_29_PENALTY=2.191566
PRIMER_LEFT_29_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_29_SEQUENCE=TTGCAGGCATCTGTTTGACC
PRIMER_LEFT_29=795,21
PRIMER_RIGHT_29=1255,20
PRIMER_LEFT_29_TM=58.106
PRIMER_RIGHT_29_TM=62.192
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=461
PRIMER_PAIR_30_PENALTY=5.110525
PRIMER_LEFT_30_PENALTY=2.893725
PRIMER_RIGHT_30_PENALTY=2.216800
PRIMER_LEFT_30_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_30_SEQUENCE=TTTCCATAACCACGTCTTTTCC
PRIMER_LEFT_30=795,21
PRIMER_RIGHT_30=1650,22
PRIMER_LEFT_30_TM=58.106
PRIMER_RIGHT_30_TM=60.217
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=856
PRIMER_PAIR_31_PENALTY=5.125785
PRIMER_LEFT_31_PENALTY=2.893725
PRIMER_RIGHT_31_PENALTY=2.232060
PRIMER_LEFT_31_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_31_SEQUENCE=ATAACCACGTCTTTTCCATCGT
PRIMER_LEFT_31=795,21
PRIMER_RIGHT_31=1645,22
PRIMER_LEFT_31_TM=58.106
PRIMER_RIGHT_31_TM=59.768
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=8.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=851
PRIMER_PAIR_32_PENALTY=5.142799
PRIMER_LEFT_32_PENALTY=2.893725
PRIMER_RIGHT_32_PENALTY=2.249074
PRIMER_LEFT_32_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_32_SEQUENCE=ACCACGTCTTTTCCATCGTATC
PRIMER_LEFT_32=795,21
PRIMER_RIGHT_32=1642,22
PRIMER_LEFT_32_TM=58.106
PRIMER_RIGHT_32_TM=60.249
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=5.3000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=848
PRIMER_PAIR_33_PENALTY=5.212804
PRIMER_LEFT_33_PENALTY=3.021238
PRIMER_RIGHT_33_PENALTY=2.191566
PRIMER_LEFT_33_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_33_SEQUENCE=TTGCAGGCATCTGTTTGACC
PRIMER_LEFT_33=794,22
PRIMER_RIGHT_33=1255,20
PRIMER_LEFT_33_TM=58.979
PRIMER_RIGHT_33_TM=62.192
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=462
PRIMER_PAIR_34_PENALTY=5.223246
PRIMER_LEFT_34_PENALTY=3.872816
PRIMER_RIGHT_34_PENALTY=1.350430
PRIMER_LEFT_34_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_34_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_34=795,20
PRIMER_RIGHT_34=1648,21
PRIMER_LEFT_34_TM=56.127
PRIMER_RIGHT_34_TM=60.350
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=854
PRIMER_PAIR_35_PENALTY=5.238038
PRIMER_LEFT_35_PENALTY=3.021238
PRIMER_RIGHT_35_PENALTY=2.216800
PRIMER_LEFT_35_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_35_SEQUENCE=TTTCCATAACCACGTCTTTTCC
PRIMER_LEFT_35=794,22
PRIMER_RIGHT_35=1650,22
PRIMER_LEFT_35_TM=58.979
PRIMER_RIGHT_35_TM=60.217
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=857
PRIMER_PAIR_36_PENALTY=5.244263
PRIMER_LEFT_36_PENALTY=2.893725
PRIMER_RIGHT_36_PENALTY=2.350537
PRIMER_LEFT_36_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_36_SEQUENCE=CGTAAACCCGCCTTTCTCAA
PRIMER_LEFT_36=795,21
PRIMER_RIGHT_36=1493,20
PRIMER_LEFT_36_TM=58.106
PRIMER_RIGHT_36_TM=62.351
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=699
PRIMER_PAIR_37_PENALTY=5.245523
PRIMER_LEFT_37_PENALTY=4.044445
PRIMER_RIGHT_37_PENALTY=1.201078
PRIMER_LEFT_37_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_37_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_37=794,20
PRIMER_RIGHT_37=1639,21
PRIMER_LEFT_37_TM=55.956
PRIMER_RIGHT_37_TM=60.201
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=9.3000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=846
PRIMER_PAIR_38_PENALTY=5.253298
PRIMER_LEFT_38_PENALTY=3.021238
PRIMER_RIGHT_38_PENALTY=2.232060
PRIMER_LEFT_38_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_38_SEQUENCE=ATAACCACGTCTTTTCCATCGT
PRIMER_LEFT_38=794,22
PRIMER_RIGHT_38=1645,22
PRIMER_LEFT_38_TM=58.979
PRIMER_RIGHT_38_TM=59.768
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=8.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=852
PRIMER_PAIR_39_PENALTY=5.270312
PRIMER_LEFT_39_PENALTY=3.021238
PRIMER_RIGHT_39_PENALTY=2.249074
PRIMER_LEFT_39_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_39_SEQUENCE=ACCACGTCTTTTCCATCGTATC
PRIMER_LEFT_39=794,22
PRIMER_RIGHT_39=1642,22
PRIMER_LEFT_39_TM=58.979
PRIMER_RIGHT_39_TM=60.249
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=5.3000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=849
PRIMER_PAIR_40_PENALTY=5.371776
PRIMER_LEFT_40_PENALTY=3.021238
PRIMER_RIGHT_40_PENALTY=2.350537
PRIMER_LEFT_40_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_40_SEQUENCE=CGTAAACCCGCCTTTCTCAA
PRIMER_LEFT_40=794,22
PRIMER_RIGHT_40=1493,20
PRIMER_LEFT_40_TM=58.979
PRIMER_RIGHT_40_TM=62.351
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=700
PRIMER_PAIR_41_PENALTY=5.376379
PRIMER_LEFT_41_PENALTY=2.893725
PRIMER_RIGHT_41_PENALTY=2.482654
PRIMER_LEFT_41_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_41_SEQUENCE=TCCATAACCACGTCTTTTCC
PRIMER_LEFT_41=795,21
PRIMER_RIGHT_41=1648,20
PRIMER_LEFT_41_TM=58.106
PRIMER_RIGHT_41_TM=57.517
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=854
PRIMER_PAIR_42_PENALTY=5.388017
PRIMER_LEFT_42_PENALTY=3.872816
PRIMER_RIGHT_42_PENALTY=1.515201
PRIMER_LEFT_42_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_42_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_42=795,20
PRIMER_RIGHT_42=1644,21
PRIMER_LEFT_42_TM=56.127
PRIMER_RIGHT_42_TM=59.485
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=8.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=850
PRIMER_PAIR_43_PENALTY=5.394876
PRIMER_LEFT_43_PENALTY=4.044445
PRIMER_RIGHT_43_PENALTY=1.350430
PRIMER_LEFT_43_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_43_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_43=794,20
PRIMER_RIGHT_43=1648,21
PRIMER_LEFT_43_TM=55.956
PRIMER_RIGHT_43_TM=60.350
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=855
PRIMER_PAIR_44_PENALTY=5.398284
PRIMER_LEFT_44_PENALTY=3.872816
PRIMER_RIGHT_44_PENALTY=1.525468
PRIMER_LEFT_44_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_44_SEQUENCE=CCATAACCACGTCTTTTCCA
PRIMER_LEFT_44=795,20
PRIMER_RIGHT_44=1647,20
PRIMER_LEFT_44_TM=56.127
PRIMER_RIGHT_44_TM=58.475
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=853
PRIMER_PAIR_45_PENALTY=5.429584
PRIMER_LEFT_45_PENALTY=3.872816
PRIMER_RIGHT_45_PENALTY=1.556768
PRIMER_LEFT_45_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_45_SEQUENCE=CCACGTCTTTTCCATCGTATC
PRIMER_LEFT_45=795,20
PRIMER_RIGHT_45=1641,21
PRIMER_LEFT_45_TM=56.127
PRIMER_RIGHT_45_TM=59.443
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=5.3000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=847
PRIMER_PAIR_46_PENALTY=5.486405
PRIMER_LEFT_46_PENALTY=2.893725
PRIMER_RIGHT_46_PENALTY=2.592680
PRIMER_LEFT_46_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_46_SEQUENCE=CCATAACCACGTCTTTTCCATC
PRIMER_LEFT_46=795,21
PRIMER_RIGHT_46=1647,22
PRIMER_LEFT_46_TM=58.106
PRIMER_RIGHT_46_TM=60.593
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=853
PRIMER_PAIR_47_PENALTY=5.503892
PRIMER_LEFT_47_PENALTY=3.021238
PRIMER_RIGHT_47_PENALTY=2.482654
PRIMER_LEFT_47_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_47_SEQUENCE=TCCATAACCACGTCTTTTCC
PRIMER_LEFT_47=794,22
PRIMER_RIGHT_47=1648,20
PRIMER_LEFT_47_TM=58.979
PRIMER_RIGHT_47_TM=57.517
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=855
PRIMER_PAIR_48_PENALTY=5.528062
PRIMER_LEFT_48_PENALTY=2.893725
PRIMER_RIGHT_48_PENALTY=2.634337
PRIMER_LEFT_48_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_48_SEQUENCE=CATCGTATCCCGAGCTAAAGT
PRIMER_LEFT_48=795,21
PRIMER_RIGHT_48=1629,21
PRIMER_LEFT_48_TM=58.106
PRIMER_RIGHT_48_TM=58.366
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=835
PRIMER_PAIR_49_PENALTY=5.559647
PRIMER_LEFT_49_PENALTY=4.044445
PRIMER_RIGHT_49_PENALTY=1.515201
PRIMER_LEFT_49_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_49_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_49=794,20
PRIMER_RIGHT_49=1644,21
PRIMER_LEFT_49_TM=55.956
PRIMER_RIGHT_49_TM=59.485
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=851
=
SEQUENCE_ID=MAGIC.446_3_6490573_6490952_bur-0_hi-0_24442_6748_TRUE
SEQUENCE_TARGET=950,479
SEQUENCE_TEMPLATE=GACTAGTATGAANGATGTCTTCACTACTCCTACNGCTACAGTCGCTAGAAGCTGTTGGTGATCGGTTTTGAGTCCGGCGGTTTTGAAAATTCGCGGTGAGAAGAGAACCACCGCGTCGATACCGGAAGCTTGCTGGAAGAAATGGATTCCGATTGCGGCGATCATNACACGACGCACNGCTGGNGTTGGTCGGATTAANAGCTCTCTCCAAACTCCTTCTCCATGACTGTTCCTCCTCGACACCTGAANNNNNTGNGAAATTAGGTNNAAATCTGGCCGNANNNNNNNNNNNNNNNAANNNNNNNNNTTNAAATTANGTCAAAATGTGNCCNGAGAAATNNCGANTTCNAANTCAATNNATNNNAATCTCGATTCGGATTTCAATTNGTGNNNNGTGTNACCTGAACGACGTCGTCGTGGCAATCGGCNGGAATACCAGCNGCGTGTTTGATGTCTTCGAGACGGAGAGTTGCTTCGGTGGGGGAATCAGAGGTTTTGTCNAGAACGCGNTTGGCATCNCCGAGACGGCCTTGCATAACGAGCCACCGTGGAGATTCCGGCATAGCGAGAACGCCGATAGCGAGTATCACGGAAGGTACGGCTCCGATTCCGAGCATCAGTCTCCATCCTACCTTCAACGGCAAGTTNGAGAAAGCCAGATTTGATACGTACCCGAGCATAATTCCGGCGTTGATAAACACCTGTACACGTCACCANAANCACTGCCACGTCAGATATTACTTTCATTCNTNTTTTTAGTNTGAATNAAAGTCAAAATAAACCGCCAAATTGTTTGGTNATTTTTTTNAATGATAATTAAATTTTATTTCAAGGTTNATAAAANAATTATAATCTGGTCAACTTTAACAGTTTAGTAACACTNAAAAGCGTNTAANTNAAAAAAAANTAGTTTACGATTATTATGTTTTATGNNNNNNNNNNNNNNNNNNNTTGTTTAAGTAANNNNNNNNNNNNNNATGTTTTGAAATTTTTANGTAANNTATTATTGNNNTTTTTACCTCGGGGAAGGAATTNAGGAAGCCGCGGGAAGATGCCGGAGAGACTTCGGCGGTATAAACTGGTGCGATCATGAGAGCATATCCGACGCCGATACCGGCNATNAACCTTCCGAACATGAGGAAAGCGTAGTTAGGGGACAATCCCATAAGGATAGCTCCGGCGAAGAATATGGCTCCAGCCAACACGATAGTGTAACGCCGACCAATCCANTCAGAGGTTCTTCCGGCGGCGCAAGANCCTATAAGTGAATAGATNTTGAGGCTTCCAGCGAGAATCCCGATTTGTAAATCATTAATCTTCAAATCTCTCTTTATATAAATCATTGCTCCACTCATCACTCCTATATCTGCAAAATATACNCTTAANNNAGAATCAATAAAGANTATNTNTGNGTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGAGTTCNACGAGAAAACTANACCATAACCAAGGAGGATAGAAGTCATGGAAGCTAAGATAGCACACGCNAATGCATAATTGTTCCTCTTTGGTGGTTTAGCCGGCAGAACTGACTCNNGNACATGTTTCANCGGTGGTGGCGACGGGGCCGTTCGGTTTTCCGGTGTGGCACCTNTCATTATGATAGAGAATGGGNNGNGAGAGAGATAGAGANTTGTGAGGATTAATGAGTAGACTAACAACAACTTGTGGTAAGGAGCNNTGTGTCTTATATAGAACANACGTGTGTGTATTCAGAGTCCAATGAGACAAGGGCAAAATCGTCCGAGAGTATGGGAAAACTGTCCTNAAATATCGGTATCATTNCATTNGNTTTTTGTTCTTGGTTAAAGAACATTTGCACGTTTCGTTTAGAGACAAAACAAAAACATTTTATCAGTAGAACAAACGGCAACTAATGATACATAAATTTCAGTTTAATATGNAANNAACTNTNNATTATTGAATTATACTCTACCAACAAAAAGGACGACCACNAATAATAATAATAAATTGNAGGGCCTAAAGGTAATTACGAAAGGATACTTGTTGTCCATGACTCCATGTGAGCTTGTTGAATGTTGACTGTTGTTGTCGACTTGATCTTGAGGATTGAGCTCATANNACANCTCAAATANTTTTANTTTATTTCAATTTGTAATATNNTATATCCTTCTGTAACATATTTAATGGTGAAAACAAGANTTTTNTNNNNAAAACACAACGAGATAATTAATATGTTTGTTTGANATGATAAGTGCTANTTTATGATATACNTATACACTGCCAATAATTTACACTGAGATTCGNTCCAATGACATTACAGNTTATTTATTTAANTNCCTGATGAACCCATAAATATTAATTGNCTNTTGTTTTCTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.925332
PRIMER_LEFT_0_PENALTY=0.095771
PRIMER_RIGHT_0_PENALTY=0.829561
PRIMER_LEFT_0_SEQUENCE=TATCACGGAAGGTACGGCTC
PRIMER_RIGHT_0_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_0=584,20
PRIMER_RIGHT_0=1559,20
PRIMER_LEFT_0_TM=60.096
PRIMER_RIGHT_0_TM=59.170
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=976
PRIMER_PAIR_1_PENALTY=1.061622
PRIMER_LEFT_1_PENALTY=0.232061
PRIMER_RIGHT_1_PENALTY=0.829561
PRIMER_LEFT_1_SEQUENCE=GATTCCGAGCATCAGTCTCC
PRIMER_RIGHT_1_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_1=605,20
PRIMER_RIGHT_1=1559,20
PRIMER_LEFT_1_TM=59.768
PRIMER_RIGHT_1_TM=59.170
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=955
PRIMER_PAIR_2_PENALTY=1.095276
PRIMER_LEFT_2_PENALTY=0.265715
PRIMER_RIGHT_2_PENALTY=0.829561
PRIMER_LEFT_2_SEQUENCE=TCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_2_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_2=626,20
PRIMER_RIGHT_2=1559,20
PRIMER_LEFT_2_TM=59.734
PRIMER_RIGHT_2_TM=59.170
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=934
PRIMER_PAIR_3_PENALTY=1.564481
PRIMER_LEFT_3_PENALTY=0.095771
PRIMER_RIGHT_3_PENALTY=1.468710
PRIMER_LEFT_3_SEQUENCE=TATCACGGAAGGTACGGCTC
PRIMER_RIGHT_3_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_3=584,20
PRIMER_RIGHT_3=1559,21
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.469
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=976
PRIMER_PAIR_4_PENALTY=1.598350
PRIMER_LEFT_4_PENALTY=0.768789
PRIMER_RIGHT_4_PENALTY=0.829561
PRIMER_LEFT_4_SEQUENCE=ATTCCGAGCATCAGTCTCCA
PRIMER_RIGHT_4_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_4=606,20
PRIMER_RIGHT_4=1559,20
PRIMER_LEFT_4_TM=60.769
PRIMER_RIGHT_4_TM=59.170
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=954
PRIMER_PAIR_5_PENALTY=1.700771
PRIMER_LEFT_5_PENALTY=0.232061
PRIMER_RIGHT_5_PENALTY=1.468710
PRIMER_LEFT_5_SEQUENCE=GATTCCGAGCATCAGTCTCC
PRIMER_RIGHT_5_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_5=605,20
PRIMER_RIGHT_5=1559,21
PRIMER_LEFT_5_TM=59.768
PRIMER_RIGHT_5_TM=60.469
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=955
PRIMER_PAIR_6_PENALTY=1.734425
PRIMER_LEFT_6_PENALTY=0.265715
PRIMER_RIGHT_6_PENALTY=1.468710
PRIMER_LEFT_6_SEQUENCE=TCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_6_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_6=626,20
PRIMER_RIGHT_6=1559,21
PRIMER_LEFT_6_TM=59.734
PRIMER_RIGHT_6_TM=60.469
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=934
PRIMER_PAIR_7_PENALTY=1.749521
PRIMER_LEFT_7_PENALTY=0.919960
PRIMER_RIGHT_7_PENALTY=0.829561
PRIMER_LEFT_7_SEQUENCE=GTATCACGGAAGGTACGGCT
PRIMER_RIGHT_7_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_7=583,20
PRIMER_RIGHT_7=1559,20
PRIMER_LEFT_7_TM=59.080
PRIMER_RIGHT_7_TM=59.170
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=11.4000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=977
PRIMER_PAIR_8_PENALTY=1.841180
PRIMER_LEFT_8_PENALTY=1.011619
PRIMER_RIGHT_8_PENALTY=0.829561
PRIMER_LEFT_8_SEQUENCE=ATCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_8_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_8=625,21
PRIMER_RIGHT_8=1559,20
PRIMER_LEFT_8_TM=60.012
PRIMER_RIGHT_8_TM=59.170
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=935
PRIMER_PAIR_9_PENALTY=1.850266
PRIMER_LEFT_9_PENALTY=1.020705
PRIMER_RIGHT_9_PENALTY=0.829561
PRIMER_LEFT_9_SEQUENCE=CATCCTACCTTCAACGGCAA
PRIMER_RIGHT_9_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_9=624,20
PRIMER_RIGHT_9=1559,20
PRIMER_LEFT_9_TM=61.021
PRIMER_RIGHT_9_TM=59.170
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=10.0000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=936
PRIMER_PAIR_10_PENALTY=1.862305
PRIMER_LEFT_10_PENALTY=1.032744
PRIMER_RIGHT_10_PENALTY=0.829561
PRIMER_LEFT_10_SEQUENCE=AGTATCACGGAAGGTACGGCT
PRIMER_RIGHT_10_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_10=582,21
PRIMER_RIGHT_10=1559,20
PRIMER_LEFT_10_TM=60.033
PRIMER_RIGHT_10_TM=59.170
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=978
PRIMER_PAIR_11_PENALTY=1.899116
PRIMER_LEFT_11_PENALTY=1.069554
PRIMER_RIGHT_11_PENALTY=0.829561
PRIMER_LEFT_11_SEQUENCE=ACGTACCCGAGCATAATTCC
PRIMER_RIGHT_11_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_11=666,20
PRIMER_RIGHT_11=1559,20
PRIMER_LEFT_11_TM=58.930
PRIMER_RIGHT_11_TM=59.170
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=894
PRIMER_PAIR_12_PENALTY=1.934953
PRIMER_LEFT_12_PENALTY=1.105391
PRIMER_RIGHT_12_PENALTY=0.829561
PRIMER_LEFT_12_SEQUENCE=CGATAGCGAGTATCACGGAA
PRIMER_RIGHT_12_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_12=574,20
PRIMER_RIGHT_12=1559,20
PRIMER_LEFT_12_TM=58.895
PRIMER_RIGHT_12_TM=59.170
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.2000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=986
PRIMER_PAIR_13_PENALTY=1.944475
PRIMER_LEFT_13_PENALTY=0.095771
PRIMER_RIGHT_13_PENALTY=1.848704
PRIMER_LEFT_13_SEQUENCE=TATCACGGAAGGTACGGCTC
PRIMER_RIGHT_13_SEQUENCE=GCGTGTGCTATCTTAGCTTCC
PRIMER_LEFT_13=584,20
PRIMER_RIGHT_13=1525,21
PRIMER_LEFT_13_TM=60.096
PRIMER_RIGHT_13_TM=59.151
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=942
PRIMER_PAIR_14_PENALTY=1.954606
PRIMER_LEFT_14_PENALTY=1.125045
PRIMER_RIGHT_14_PENALTY=0.829561
PRIMER_LEFT_14_SEQUENCE=CGATAGCGAGTATCACGGAAG
PRIMER_RIGHT_14_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_14=574,21
PRIMER_RIGHT_14=1559,20
PRIMER_LEFT_14_TM=59.875
PRIMER_RIGHT_14_TM=59.170
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=986
PRIMER_PAIR_15_PENALTY=1.976977
PRIMER_LEFT_15_PENALTY=0.095771
PRIMER_RIGHT_15_PENALTY=1.881206
PRIMER_LEFT_15_SEQUENCE=TATCACGGAAGGTACGGCTC
PRIMER_RIGHT_15_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_15=584,20
PRIMER_RIGHT_15=1560,20
PRIMER_LEFT_15_TM=60.096
PRIMER_RIGHT_15_TM=61.881
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=977
PRIMER_PAIR_16_PENALTY=1.976977
PRIMER_LEFT_16_PENALTY=0.095771
PRIMER_RIGHT_16_PENALTY=1.881206
PRIMER_LEFT_16_SEQUENCE=TATCACGGAAGGTACGGCTC
PRIMER_RIGHT_16_SEQUENCE=CCGGCTAAACCACCAAAGAG
PRIMER_LEFT_16=584,20
PRIMER_RIGHT_16=1561,20
PRIMER_LEFT_16_TM=60.096
PRIMER_RIGHT_16_TM=61.881
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=978
PRIMER_PAIR_17_PENALTY=2.080766
PRIMER_LEFT_17_PENALTY=0.232061
PRIMER_RIGHT_17_PENALTY=1.848704
PRIMER_LEFT_17_SEQUENCE=GATTCCGAGCATCAGTCTCC
PRIMER_RIGHT_17_SEQUENCE=GCGTGTGCTATCTTAGCTTCC
PRIMER_LEFT_17=605,20
PRIMER_RIGHT_17=1525,21
PRIMER_LEFT_17_TM=59.768
PRIMER_RIGHT_17_TM=59.151
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=921
PRIMER_PAIR_18_PENALTY=2.113267
PRIMER_LEFT_18_PENALTY=0.232061
PRIMER_RIGHT_18_PENALTY=1.881206
PRIMER_LEFT_18_SEQUENCE=GATTCCGAGCATCAGTCTCC
PRIMER_RIGHT_18_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_18=605,20
PRIMER_RIGHT_18=1560,20
PRIMER_LEFT_18_TM=59.768
PRIMER_RIGHT_18_TM=61.881
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=956
PRIMER_PAIR_19_PENALTY=2.113267
PRIMER_LEFT_19_PENALTY=0.232061
PRIMER_RIGHT_19_PENALTY=1.881206
PRIMER_LEFT_19_SEQUENCE=GATTCCGAGCATCAGTCTCC
PRIMER_RIGHT_19_SEQUENCE=CCGGCTAAACCACCAAAGAG
PRIMER_LEFT_19=605,20
PRIMER_RIGHT_19=1561,20
PRIMER_LEFT_19_TM=59.768
PRIMER_RIGHT_19_TM=61.881
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=957
PRIMER_PAIR_20_PENALTY=2.114420
PRIMER_LEFT_20_PENALTY=0.265715
PRIMER_RIGHT_20_PENALTY=1.848704
PRIMER_LEFT_20_SEQUENCE=TCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_20_SEQUENCE=GCGTGTGCTATCTTAGCTTCC
PRIMER_LEFT_20=626,20
PRIMER_RIGHT_20=1525,21
PRIMER_LEFT_20_TM=59.734
PRIMER_RIGHT_20_TM=59.151
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=900
PRIMER_PAIR_21_PENALTY=2.146921
PRIMER_LEFT_21_PENALTY=0.265715
PRIMER_RIGHT_21_PENALTY=1.881206
PRIMER_LEFT_21_SEQUENCE=TCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_21_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_21=626,20
PRIMER_RIGHT_21=1560,20
PRIMER_LEFT_21_TM=59.734
PRIMER_RIGHT_21_TM=61.881
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=935
PRIMER_PAIR_22_PENALTY=2.146921
PRIMER_LEFT_22_PENALTY=0.265715
PRIMER_RIGHT_22_PENALTY=1.881206
PRIMER_LEFT_22_SEQUENCE=TCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_22_SEQUENCE=CCGGCTAAACCACCAAAGAG
PRIMER_LEFT_22=626,20
PRIMER_RIGHT_22=1561,20
PRIMER_LEFT_22_TM=59.734
PRIMER_RIGHT_22_TM=61.881
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=936
PRIMER_PAIR_23_PENALTY=2.177949
PRIMER_LEFT_23_PENALTY=1.348388
PRIMER_RIGHT_23_PENALTY=0.829561
PRIMER_LEFT_23_SEQUENCE=TCCGAGCATCAGTCTCCATC
PRIMER_RIGHT_23_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_23=608,20
PRIMER_RIGHT_23=1559,20
PRIMER_LEFT_23_TM=61.348
PRIMER_RIGHT_23_TM=59.170
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=952
PRIMER_PAIR_24_PENALTY=2.237499
PRIMER_LEFT_24_PENALTY=0.768789
PRIMER_RIGHT_24_PENALTY=1.468710
PRIMER_LEFT_24_SEQUENCE=ATTCCGAGCATCAGTCTCCA
PRIMER_RIGHT_24_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_24=606,20
PRIMER_RIGHT_24=1559,21
PRIMER_LEFT_24_TM=60.769
PRIMER_RIGHT_24_TM=60.469
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=954
PRIMER_PAIR_25_PENALTY=2.388670
PRIMER_LEFT_25_PENALTY=0.919960
PRIMER_RIGHT_25_PENALTY=1.468710
PRIMER_LEFT_25_SEQUENCE=GTATCACGGAAGGTACGGCT
PRIMER_RIGHT_25_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_25=583,20
PRIMER_RIGHT_25=1559,21
PRIMER_LEFT_25_TM=59.080
PRIMER_RIGHT_25_TM=60.469
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=11.4000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=977
PRIMER_PAIR_26_PENALTY=2.480329
PRIMER_LEFT_26_PENALTY=1.011619
PRIMER_RIGHT_26_PENALTY=1.468710
PRIMER_LEFT_26_SEQUENCE=ATCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_26_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_26=625,21
PRIMER_RIGHT_26=1559,21
PRIMER_LEFT_26_TM=60.012
PRIMER_RIGHT_26_TM=60.469
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=935
PRIMER_PAIR_27_PENALTY=2.489415
PRIMER_LEFT_27_PENALTY=1.020705
PRIMER_RIGHT_27_PENALTY=1.468710
PRIMER_LEFT_27_SEQUENCE=CATCCTACCTTCAACGGCAA
PRIMER_RIGHT_27_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_27=624,20
PRIMER_RIGHT_27=1559,21
PRIMER_LEFT_27_TM=61.021
PRIMER_RIGHT_27_TM=60.469
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=10.0000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=936
PRIMER_PAIR_28_PENALTY=2.501454
PRIMER_LEFT_28_PENALTY=1.032744
PRIMER_RIGHT_28_PENALTY=1.468710
PRIMER_LEFT_28_SEQUENCE=AGTATCACGGAAGGTACGGCT
PRIMER_RIGHT_28_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_28=582,21
PRIMER_RIGHT_28=1559,21
PRIMER_LEFT_28_TM=60.033
PRIMER_RIGHT_28_TM=60.469
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=11.4000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=978
PRIMER_PAIR_29_PENALTY=2.574102
PRIMER_LEFT_29_PENALTY=1.105391
PRIMER_RIGHT_29_PENALTY=1.468710
PRIMER_LEFT_29_SEQUENCE=CGATAGCGAGTATCACGGAA
PRIMER_RIGHT_29_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_29=574,20
PRIMER_RIGHT_29=1559,21
PRIMER_LEFT_29_TM=58.895
PRIMER_RIGHT_29_TM=60.469
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=10.2000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=986
PRIMER_PAIR_30_PENALTY=2.593755
PRIMER_LEFT_30_PENALTY=1.125045
PRIMER_RIGHT_30_PENALTY=1.468710
PRIMER_LEFT_30_SEQUENCE=CGATAGCGAGTATCACGGAAG
PRIMER_RIGHT_30_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_30=574,21
PRIMER_RIGHT_30=1559,21
PRIMER_LEFT_30_TM=59.875
PRIMER_RIGHT_30_TM=60.469
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=986
PRIMER_PAIR_31_PENALTY=2.609602
PRIMER_LEFT_31_PENALTY=0.095771
PRIMER_RIGHT_31_PENALTY=2.513831
PRIMER_LEFT_31_SEQUENCE=TATCACGGAAGGTACGGCTC
PRIMER_RIGHT_31_SEQUENCE=GCTAAACCACCAAAGAGGAACA
PRIMER_LEFT_31=584,20
PRIMER_RIGHT_31=1558,22
PRIMER_LEFT_31_TM=60.096
PRIMER_RIGHT_31_TM=60.514
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=975
PRIMER_PAIR_32_PENALTY=2.617493
PRIMER_LEFT_32_PENALTY=0.768789
PRIMER_RIGHT_32_PENALTY=1.848704
PRIMER_LEFT_32_SEQUENCE=ATTCCGAGCATCAGTCTCCA
PRIMER_RIGHT_32_SEQUENCE=GCGTGTGCTATCTTAGCTTCC
PRIMER_LEFT_32=606,20
PRIMER_RIGHT_32=1525,21
PRIMER_LEFT_32_TM=60.769
PRIMER_RIGHT_32_TM=59.151
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=920
PRIMER_PAIR_33_PENALTY=2.649995
PRIMER_LEFT_33_PENALTY=0.768789
PRIMER_RIGHT_33_PENALTY=1.881206
PRIMER_LEFT_33_SEQUENCE=ATTCCGAGCATCAGTCTCCA
PRIMER_RIGHT_33_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_33=606,20
PRIMER_RIGHT_33=1560,20
PRIMER_LEFT_33_TM=60.769
PRIMER_RIGHT_33_TM=61.881
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=955
PRIMER_PAIR_34_PENALTY=2.649995
PRIMER_LEFT_34_PENALTY=0.768789
PRIMER_RIGHT_34_PENALTY=1.881206
PRIMER_LEFT_34_SEQUENCE=ATTCCGAGCATCAGTCTCCA
PRIMER_RIGHT_34_SEQUENCE=CCGGCTAAACCACCAAAGAG
PRIMER_LEFT_34=606,20
PRIMER_RIGHT_34=1561,20
PRIMER_LEFT_34_TM=60.769
PRIMER_RIGHT_34_TM=61.881
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=956
PRIMER_PAIR_35_PENALTY=2.719291
PRIMER_LEFT_35_PENALTY=1.889730
PRIMER_RIGHT_35_PENALTY=0.829561
PRIMER_LEFT_35_SEQUENCE=GTATCACGGAAGGTACGGCTC
PRIMER_RIGHT_35_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_35=583,21
PRIMER_RIGHT_35=1559,20
PRIMER_LEFT_35_TM=60.890
PRIMER_RIGHT_35_TM=59.170
PRIMER_LEFT_35_GC_PERCENT=57.143
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=977
PRIMER_PAIR_36_PENALTY=2.745893
PRIMER_LEFT_36_PENALTY=0.232061
PRIMER_RIGHT_36_PENALTY=2.513831
PRIMER_LEFT_36_SEQUENCE=GATTCCGAGCATCAGTCTCC
PRIMER_RIGHT_36_SEQUENCE=GCTAAACCACCAAAGAGGAACA
PRIMER_LEFT_36=605,20
PRIMER_RIGHT_36=1558,22
PRIMER_LEFT_36_TM=59.768
PRIMER_RIGHT_36_TM=60.514
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=954
PRIMER_PAIR_37_PENALTY=2.779546
PRIMER_LEFT_37_PENALTY=0.265715
PRIMER_RIGHT_37_PENALTY=2.513831
PRIMER_LEFT_37_SEQUENCE=TCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_37_SEQUENCE=GCTAAACCACCAAAGAGGAACA
PRIMER_LEFT_37=626,20
PRIMER_RIGHT_37=1558,22
PRIMER_LEFT_37_TM=59.734
PRIMER_RIGHT_37_TM=60.514
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=933
PRIMER_PAIR_38_PENALTY=2.801166
PRIMER_LEFT_38_PENALTY=0.919960
PRIMER_RIGHT_38_PENALTY=1.881206
PRIMER_LEFT_38_SEQUENCE=GTATCACGGAAGGTACGGCT
PRIMER_RIGHT_38_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_38=583,20
PRIMER_RIGHT_38=1560,20
PRIMER_LEFT_38_TM=59.080
PRIMER_RIGHT_38_TM=61.881
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=11.4000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=978
PRIMER_PAIR_39_PENALTY=2.801166
PRIMER_LEFT_39_PENALTY=0.919960
PRIMER_RIGHT_39_PENALTY=1.881206
PRIMER_LEFT_39_SEQUENCE=GTATCACGGAAGGTACGGCT
PRIMER_RIGHT_39_SEQUENCE=CCGGCTAAACCACCAAAGAG
PRIMER_LEFT_39=583,20
PRIMER_RIGHT_39=1561,20
PRIMER_LEFT_39_TM=59.080
PRIMER_RIGHT_39_TM=61.881
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=11.4000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=979
PRIMER_PAIR_40_PENALTY=2.817098
PRIMER_LEFT_40_PENALTY=1.348388
PRIMER_RIGHT_40_PENALTY=1.468710
PRIMER_LEFT_40_SEQUENCE=TCCGAGCATCAGTCTCCATC
PRIMER_RIGHT_40_SEQUENCE=GGCTAAACCACCAAAGAGGAA
PRIMER_LEFT_40=608,20
PRIMER_RIGHT_40=1559,21
PRIMER_LEFT_40_TM=61.348
PRIMER_RIGHT_40_TM=60.469
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=952
PRIMER_PAIR_41_PENALTY=2.850308
PRIMER_LEFT_41_PENALTY=2.020747
PRIMER_RIGHT_41_PENALTY=0.829561
PRIMER_LEFT_41_SEQUENCE=TACGTACCCGAGCATAATTCC
PRIMER_RIGHT_41_SEQUENCE=GGCTAAACCACCAAAGAGGA
PRIMER_LEFT_41=665,21
PRIMER_RIGHT_41=1559,20
PRIMER_LEFT_41_TM=58.979
PRIMER_RIGHT_41_TM=59.170
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=895
PRIMER_PAIR_42_PENALTY=2.860323
PRIMER_LEFT_42_PENALTY=1.011619
PRIMER_RIGHT_42_PENALTY=1.848704
PRIMER_LEFT_42_SEQUENCE=ATCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_42_SEQUENCE=GCGTGTGCTATCTTAGCTTCC
PRIMER_LEFT_42=625,21
PRIMER_RIGHT_42=1525,21
PRIMER_LEFT_42_TM=60.012
PRIMER_RIGHT_42_TM=59.151
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=901
PRIMER_PAIR_43_PENALTY=2.869410
PRIMER_LEFT_43_PENALTY=1.020705
PRIMER_RIGHT_43_PENALTY=1.848704
PRIMER_LEFT_43_SEQUENCE=CATCCTACCTTCAACGGCAA
PRIMER_RIGHT_43_SEQUENCE=GCGTGTGCTATCTTAGCTTCC
PRIMER_LEFT_43=624,20
PRIMER_RIGHT_43=1525,21
PRIMER_LEFT_43_TM=61.021
PRIMER_RIGHT_43_TM=59.151
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=10.0000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=902
PRIMER_PAIR_44_PENALTY=2.892825
PRIMER_LEFT_44_PENALTY=1.011619
PRIMER_RIGHT_44_PENALTY=1.881206
PRIMER_LEFT_44_SEQUENCE=ATCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_44_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_44=625,21
PRIMER_RIGHT_44=1560,20
PRIMER_LEFT_44_TM=60.012
PRIMER_RIGHT_44_TM=61.881
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=936
PRIMER_PAIR_45_PENALTY=2.892825
PRIMER_LEFT_45_PENALTY=1.011619
PRIMER_RIGHT_45_PENALTY=1.881206
PRIMER_LEFT_45_SEQUENCE=ATCCTACCTTCAACGGCAAGT
PRIMER_RIGHT_45_SEQUENCE=CCGGCTAAACCACCAAAGAG
PRIMER_LEFT_45=625,21
PRIMER_RIGHT_45=1561,20
PRIMER_LEFT_45_TM=60.012
PRIMER_RIGHT_45_TM=61.881
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=937
PRIMER_PAIR_46_PENALTY=2.897898
PRIMER_LEFT_46_PENALTY=0.095771
PRIMER_RIGHT_46_PENALTY=2.802127
PRIMER_LEFT_46_SEQUENCE=TATCACGGAAGGTACGGCTC
PRIMER_RIGHT_46_SEQUENCE=GCTTCCATGACTTCTATCCTCC
PRIMER_LEFT_46=584,20
PRIMER_RIGHT_46=1510,22
PRIMER_LEFT_46_TM=60.096
PRIMER_RIGHT_46_TM=59.198
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=927
PRIMER_PAIR_47_PENALTY=2.901911
PRIMER_LEFT_47_PENALTY=1.020705
PRIMER_RIGHT_47_PENALTY=1.881206
PRIMER_LEFT_47_SEQUENCE=CATCCTACCTTCAACGGCAA
PRIMER_RIGHT_47_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_47=624,20
PRIMER_RIGHT_47=1560,20
PRIMER_LEFT_47_TM=61.021
PRIMER_RIGHT_47_TM=61.881
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=10.0000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=937
PRIMER_PAIR_48_PENALTY=2.901911
PRIMER_LEFT_48_PENALTY=1.020705
PRIMER_RIGHT_48_PENALTY=1.881206
PRIMER_LEFT_48_SEQUENCE=CATCCTACCTTCAACGGCAA
PRIMER_RIGHT_48_SEQUENCE=CCGGCTAAACCACCAAAGAG
PRIMER_LEFT_48=624,20
PRIMER_RIGHT_48=1561,20
PRIMER_LEFT_48_TM=61.021
PRIMER_RIGHT_48_TM=61.881
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=10.0000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=938
PRIMER_PAIR_49_PENALTY=2.913951
PRIMER_LEFT_49_PENALTY=1.032744
PRIMER_RIGHT_49_PENALTY=1.881206
PRIMER_LEFT_49_SEQUENCE=AGTATCACGGAAGGTACGGCT
PRIMER_RIGHT_49_SEQUENCE=CGGCTAAACCACCAAAGAGG
PRIMER_LEFT_49=582,21
PRIMER_RIGHT_49=1560,20
PRIMER_LEFT_49_TM=60.033
PRIMER_RIGHT_49_TM=61.881
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=11.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=979
=
SEQUENCE_ID=MAGIC.338_2_5541594_5541971_col-0_tsu-0_489332_118850_TRUE
SEQUENCE_TARGET=950,477
SEQUENCE_TEMPLATE=CTGCTGGTCAATCCAGAGCAAGCTAAATTGTCCAATCAGAAACGAAACAATCCTGTCTCCAGGAGATAACGTGTTGGTCAAGGCACTCTCCCAAGCACCAGTCCCGGTCGTGGGAAACAGAAAAGGTGTCCCTGATGTGGTCTTGAATATCTTCTTAACATCCTCCAACAATGTTTTCGTAAGCGCCGGAATGGCTGGTGACCGGTAATCCTCGTTGTTCCGGTTCATCGCCCGGATTACCGGTTCCGGTATGTTCACTGGTCCTGGTACAAACAGATGGTGTCTCCCTGGTCCATACATATAGTCCATTTTTTCCTCTTTTCTTTTGGATCCTCCTCTNCACTGTCAAAANCTTACAAACTCACAATCTCATCTTTCACCAGATAATCGAATATAACTCAAACATGTAGCCATATGCATATATGACCATGTAATATGTACTATTGCTNCTATCAACTCCGGTGAACAATAACAAATGTTNTTTTTTTTGTTAATAACAATAACAAATATGTTAGCCGATCATATTTCCAATAAGACAACATATATGTGTACTATAAAAAGATGTTTCATAGGCAAGATCNTAGACACAAACAAGCGGAGAAAACGAAGAAGTAAATTTGCAAGGAAGAGAGAAGTTTAACCTCTGAATAAGGCGGAAGCTCCTGAGGGAGGTTTTGGACTCCTTCGTCTTCACAAACCTCTTTAATATATCTTCNNNNNNNNNTTNTTTTTNTTNNNNNNNNNNATTTTNAATTACATGATGTTTTTGTAAGTAGTTTATTTTGTTATTTAGTTTATCCCAATCAAGTAGCTTGCCTTATTGGAATCTTGGCCGCGTGGCAGATAAGAGCAACGTNAAAAATCTTTGAGCTTACGTATGGCTCACAATGCATTTGTGTTACCTTTGCATATTTGCCCTTCTTCTCTCTTATATTTTTNAGTTTTTCCNCATTAGTTTGTTTTGTCGTGTTCAAAATAANAATAATCAAATCACTCTTTTNTTTNAATCAGAGATCAANANNCTCTTACCACTGACTAATCTTCTTAAAANCTNTTGGTGAGTGTNCCCGCCGTATAACCCGCTTACTAAATTNGTTTATNGACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATTAGTAAGTCATNCATANATGCAACCTATGTTTACTTAGCCACCACATTTTTTTCTGTAAAATTCAGAATGAAAANAGGATGATANTTGGATAAACCAAGAGGTTNTTGGTCTAATAGTTANTTTNAAAGATGANAATTTTGATTCGAATNCACTTAGTAGGAAAGTTGTTTCCAAAAAAGNNTCNGAACGGTGATCNAACCGCATTGTGCGGTGCAGTGTATGTAGATATTTTCGTNANTATTAGAANTGCATTGTACTTTATCCGCAGTTGAACNGNACGTTACTATTCTNATCCANGNANATTAGGATCCATCACGAATNTTTTGGCAAAATATATATTTATATAATGAGAGAGTGGGNTGGGAGACGGAGTTGACTTTGAAAAGGTTTGGGNATAAAACAAAANNTATAANGGAATTAGGGANGGATATTGTAATATAAGAAAGTAAGAAAGATAAGAGGTGGAAAAGGGAAGAGGAGAGATAAGGAGATAAAAGGGAGAGAGCCACACAACCTGTTTTNGCAACAAAGACCACACGACCAAACGCCGAATCCTCGGGGCACTTCTTCACTATCTCTCGCTCTCTTGTTTTATGTTTAGGATTATTATTGCACTAGACGACACATAATTCAAACTATATTTAGGTGAATATCTATTGAATGTATAGAATATGGAGACTTTGTAAAATACAAACTATATTTGGCCATACGATTAGGATGTTTGAATTTCTTTTGCATAAAAAAATTGNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.681697
PRIMER_LEFT_0_PENALTY=0.453004
PRIMER_RIGHT_0_PENALTY=0.228693
PRIMER_LEFT_0_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_0_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_0=901,20
PRIMER_RIGHT_0=1841,20
PRIMER_LEFT_0_TM=59.547
PRIMER_RIGHT_0_TM=59.771
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=941
PRIMER_PAIR_1_PENALTY=1.554565
PRIMER_LEFT_1_PENALTY=1.325871
PRIMER_RIGHT_1_PENALTY=0.228693
PRIMER_LEFT_1_SEQUENCE=ACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_1_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_1=900,21
PRIMER_RIGHT_1=1841,20
PRIMER_LEFT_1_TM=60.326
PRIMER_RIGHT_1_TM=59.771
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=942
PRIMER_PAIR_2_PENALTY=1.646795
PRIMER_LEFT_2_PENALTY=1.418101
PRIMER_RIGHT_2_PENALTY=0.228693
PRIMER_LEFT_2_SEQUENCE=ACCTTTGCATATTTGCCCTT
PRIMER_RIGHT_2_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_2=900,20
PRIMER_RIGHT_2=1841,20
PRIMER_LEFT_2_TM=58.582
PRIMER_RIGHT_2_TM=59.771
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=942
PRIMER_PAIR_3_PENALTY=1.649907
PRIMER_LEFT_3_PENALTY=0.453004
PRIMER_RIGHT_3_PENALTY=1.196903
PRIMER_LEFT_3_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_3_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_3=901,20
PRIMER_RIGHT_3=1843,21
PRIMER_LEFT_3_TM=59.547
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=943
PRIMER_PAIR_4_PENALTY=1.666257
PRIMER_LEFT_4_PENALTY=0.453004
PRIMER_RIGHT_4_PENALTY=1.213253
PRIMER_LEFT_4_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_4_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_4=901,20
PRIMER_RIGHT_4=1840,20
PRIMER_LEFT_4_TM=59.547
PRIMER_RIGHT_4_TM=61.213
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=940
PRIMER_PAIR_5_PENALTY=1.673959
PRIMER_LEFT_5_PENALTY=1.445265
PRIMER_RIGHT_5_PENALTY=0.228693
PRIMER_LEFT_5_SEQUENCE=CCTTTGCATATTTGCCCTTCT
PRIMER_RIGHT_5_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_5=901,21
PRIMER_RIGHT_5=1841,20
PRIMER_LEFT_5_TM=60.445
PRIMER_RIGHT_5_TM=59.771
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=941
PRIMER_PAIR_6_PENALTY=1.918562
PRIMER_LEFT_6_PENALTY=1.689868
PRIMER_RIGHT_6_PENALTY=0.228693
PRIMER_LEFT_6_SEQUENCE=TGCATATTTGCCCTTCTTCTC
PRIMER_RIGHT_6_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_6=905,21
PRIMER_RIGHT_6=1841,20
PRIMER_LEFT_6_TM=59.310
PRIMER_RIGHT_6_TM=59.771
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=937
PRIMER_PAIR_7_PENALTY=2.243591
PRIMER_LEFT_7_PENALTY=0.453004
PRIMER_RIGHT_7_PENALTY=1.790588
PRIMER_LEFT_7_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_7_SEQUENCE=CTACATACACTGCACCGCACA
PRIMER_LEFT_7=901,20
PRIMER_RIGHT_7=1842,21
PRIMER_LEFT_7_TM=59.547
PRIMER_RIGHT_7_TM=60.791
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=942
PRIMER_PAIR_8_PENALTY=2.324017
PRIMER_LEFT_8_PENALTY=2.095324
PRIMER_RIGHT_8_PENALTY=0.228693
PRIMER_LEFT_8_SEQUENCE=CTTTGCATATTTGCCCTTCTTC
PRIMER_RIGHT_8_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_8=902,22
PRIMER_RIGHT_8=1841,20
PRIMER_LEFT_8_TM=60.095
PRIMER_RIGHT_8_TM=59.771
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=940
PRIMER_PAIR_9_PENALTY=2.384610
PRIMER_LEFT_9_PENALTY=2.155917
PRIMER_RIGHT_9_PENALTY=0.228693
PRIMER_LEFT_9_SEQUENCE=TTGCATATTTGCCCTTCTTC
PRIMER_RIGHT_9_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_9=904,20
PRIMER_RIGHT_9=1841,20
PRIMER_LEFT_9_TM=57.844
PRIMER_RIGHT_9_TM=59.771
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=938
PRIMER_PAIR_10_PENALTY=2.452707
PRIMER_LEFT_10_PENALTY=2.224014
PRIMER_RIGHT_10_PENALTY=0.228693
PRIMER_LEFT_10_SEQUENCE=TGCATATTTGCCCTTCTTCTCT
PRIMER_RIGHT_10_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_10=905,22
PRIMER_RIGHT_10=1841,20
PRIMER_LEFT_10_TM=60.224
PRIMER_RIGHT_10_TM=59.771
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=937
PRIMER_PAIR_11_PENALTY=2.522774
PRIMER_LEFT_11_PENALTY=1.325871
PRIMER_RIGHT_11_PENALTY=1.196903
PRIMER_LEFT_11_SEQUENCE=ACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_11_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_11=900,21
PRIMER_RIGHT_11=1843,21
PRIMER_LEFT_11_TM=60.326
PRIMER_RIGHT_11_TM=59.803
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=944
PRIMER_PAIR_12_PENALTY=2.539125
PRIMER_LEFT_12_PENALTY=1.325871
PRIMER_RIGHT_12_PENALTY=1.213253
PRIMER_LEFT_12_SEQUENCE=ACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_12_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_12=900,21
PRIMER_RIGHT_12=1840,20
PRIMER_LEFT_12_TM=60.326
PRIMER_RIGHT_12_TM=61.213
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=941
PRIMER_PAIR_13_PENALTY=2.541439
PRIMER_LEFT_13_PENALTY=0.453004
PRIMER_RIGHT_13_PENALTY=2.088435
PRIMER_LEFT_13_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_13_SEQUENCE=ATCTACATACACTGCACCGCAC
PRIMER_LEFT_13=901,20
PRIMER_RIGHT_13=1844,22
PRIMER_LEFT_13_TM=59.547
PRIMER_RIGHT_13_TM=60.088
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=944
PRIMER_PAIR_14_PENALTY=2.553077
PRIMER_LEFT_14_PENALTY=2.324383
PRIMER_RIGHT_14_PENALTY=0.228693
PRIMER_LEFT_14_SEQUENCE=TACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_14_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_14=899,22
PRIMER_RIGHT_14=1841,20
PRIMER_LEFT_14_TM=60.324
PRIMER_RIGHT_14_TM=59.771
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=943
PRIMER_PAIR_15_PENALTY=2.615004
PRIMER_LEFT_15_PENALTY=1.418101
PRIMER_RIGHT_15_PENALTY=1.196903
PRIMER_LEFT_15_SEQUENCE=ACCTTTGCATATTTGCCCTT
PRIMER_RIGHT_15_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_15=900,20
PRIMER_RIGHT_15=1843,21
PRIMER_LEFT_15_TM=58.582
PRIMER_RIGHT_15_TM=59.803
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=944
PRIMER_PAIR_16_PENALTY=2.628908
PRIMER_LEFT_16_PENALTY=0.453004
PRIMER_RIGHT_16_PENALTY=2.175904
PRIMER_LEFT_16_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_16_SEQUENCE=TACATACACTGCACCGCACAA
PRIMER_LEFT_16=901,20
PRIMER_RIGHT_16=1841,21
PRIMER_LEFT_16_TM=59.547
PRIMER_RIGHT_16_TM=61.176
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=941
PRIMER_PAIR_17_PENALTY=2.631355
PRIMER_LEFT_17_PENALTY=1.418101
PRIMER_RIGHT_17_PENALTY=1.213253
PRIMER_LEFT_17_SEQUENCE=ACCTTTGCATATTTGCCCTT
PRIMER_RIGHT_17_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_17=900,20
PRIMER_RIGHT_17=1840,20
PRIMER_LEFT_17_TM=58.582
PRIMER_RIGHT_17_TM=61.213
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=941
PRIMER_PAIR_18_PENALTY=2.642168
PRIMER_LEFT_18_PENALTY=1.445265
PRIMER_RIGHT_18_PENALTY=1.196903
PRIMER_LEFT_18_SEQUENCE=CCTTTGCATATTTGCCCTTCT
PRIMER_RIGHT_18_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_18=901,21
PRIMER_RIGHT_18=1843,21
PRIMER_LEFT_18_TM=60.445
PRIMER_RIGHT_18_TM=59.803
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=943
PRIMER_PAIR_19_PENALTY=2.658437
PRIMER_LEFT_19_PENALTY=0.453004
PRIMER_RIGHT_19_PENALTY=2.205434
PRIMER_LEFT_19_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_19_SEQUENCE=CTACATACACTGCACCGCAC
PRIMER_LEFT_19=901,20
PRIMER_RIGHT_19=1842,20
PRIMER_LEFT_19_TM=59.547
PRIMER_RIGHT_19_TM=57.795
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=942
PRIMER_PAIR_20_PENALTY=2.658519
PRIMER_LEFT_20_PENALTY=1.445265
PRIMER_RIGHT_20_PENALTY=1.213253
PRIMER_LEFT_20_SEQUENCE=CCTTTGCATATTTGCCCTTCT
PRIMER_RIGHT_20_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_20=901,21
PRIMER_RIGHT_20=1840,20
PRIMER_LEFT_20_TM=60.445
PRIMER_RIGHT_20_TM=61.213
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=940
PRIMER_PAIR_21_PENALTY=2.772776
PRIMER_LEFT_21_PENALTY=2.544082
PRIMER_RIGHT_21_PENALTY=0.228693
PRIMER_LEFT_21_SEQUENCE=TGCATATTTGCCCTTCTTCT
PRIMER_RIGHT_21_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_21=905,20
PRIMER_RIGHT_21=1841,20
PRIMER_LEFT_21_TM=57.456
PRIMER_RIGHT_21_TM=59.771
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=937
PRIMER_PAIR_22_PENALTY=2.794667
PRIMER_LEFT_22_PENALTY=2.565974
PRIMER_RIGHT_22_PENALTY=0.228693
PRIMER_LEFT_22_SEQUENCE=TTGCATATTTGCCCTTCTTCTC
PRIMER_RIGHT_22_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_22=904,22
PRIMER_RIGHT_22=1841,20
PRIMER_LEFT_22_TM=60.566
PRIMER_RIGHT_22_TM=59.771
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=938
PRIMER_PAIR_23_PENALTY=2.808899
PRIMER_LEFT_23_PENALTY=2.580205
PRIMER_RIGHT_23_PENALTY=0.228693
PRIMER_LEFT_23_SEQUENCE=GTTACCTTTGCATATTTGCCCT
PRIMER_RIGHT_23_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_23=897,22
PRIMER_RIGHT_23=1841,20
PRIMER_LEFT_23_TM=59.420
PRIMER_RIGHT_23_TM=59.771
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=10.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=945
PRIMER_PAIR_24_PENALTY=2.870002
PRIMER_LEFT_24_PENALTY=2.641309
PRIMER_RIGHT_24_PENALTY=0.228693
PRIMER_LEFT_24_SEQUENCE=GCATATTTGCCCTTCTTCTCTC
PRIMER_RIGHT_24_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_24=906,22
PRIMER_RIGHT_24=1841,20
PRIMER_LEFT_24_TM=59.359
PRIMER_RIGHT_24_TM=59.771
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=936
PRIMER_PAIR_25_PENALTY=2.886771
PRIMER_LEFT_25_PENALTY=1.689868
PRIMER_RIGHT_25_PENALTY=1.196903
PRIMER_LEFT_25_SEQUENCE=TGCATATTTGCCCTTCTTCTC
PRIMER_RIGHT_25_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_25=905,21
PRIMER_RIGHT_25=1843,21
PRIMER_LEFT_25_TM=59.310
PRIMER_RIGHT_25_TM=59.803
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=52.381
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=939
PRIMER_PAIR_26_PENALTY=2.903122
PRIMER_LEFT_26_PENALTY=1.689868
PRIMER_RIGHT_26_PENALTY=1.213253
PRIMER_LEFT_26_SEQUENCE=TGCATATTTGCCCTTCTTCTC
PRIMER_RIGHT_26_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_26=905,21
PRIMER_RIGHT_26=1840,20
PRIMER_LEFT_26_TM=59.310
PRIMER_RIGHT_26_TM=61.213
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=936
PRIMER_PAIR_27_PENALTY=2.945473
PRIMER_LEFT_27_PENALTY=2.716780
PRIMER_RIGHT_27_PENALTY=0.228693
PRIMER_LEFT_27_SEQUENCE=TACCTTTGCATATTTGCCCT
PRIMER_RIGHT_27_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_27=899,20
PRIMER_RIGHT_27=1841,20
PRIMER_LEFT_27_TM=57.283
PRIMER_RIGHT_27_TM=59.771
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=10.9000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=943
PRIMER_PAIR_28_PENALTY=3.116459
PRIMER_LEFT_28_PENALTY=1.325871
PRIMER_RIGHT_28_PENALTY=1.790588
PRIMER_LEFT_28_SEQUENCE=ACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_28_SEQUENCE=CTACATACACTGCACCGCACA
PRIMER_LEFT_28=900,21
PRIMER_RIGHT_28=1842,21
PRIMER_LEFT_28_TM=60.326
PRIMER_RIGHT_28_TM=60.791
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=943
PRIMER_PAIR_29_PENALTY=3.208689
PRIMER_LEFT_29_PENALTY=1.418101
PRIMER_RIGHT_29_PENALTY=1.790588
PRIMER_LEFT_29_SEQUENCE=ACCTTTGCATATTTGCCCTT
PRIMER_RIGHT_29_SEQUENCE=CTACATACACTGCACCGCACA
PRIMER_LEFT_29=900,20
PRIMER_RIGHT_29=1842,21
PRIMER_LEFT_29_TM=58.582
PRIMER_RIGHT_29_TM=60.791
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=943
PRIMER_PAIR_30_PENALTY=3.210289
PRIMER_LEFT_30_PENALTY=2.981596
PRIMER_RIGHT_30_PENALTY=0.228693
PRIMER_LEFT_30_SEQUENCE=CTTTGCATATTTGCCCTTCT
PRIMER_RIGHT_30_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_30=902,20
PRIMER_RIGHT_30=1841,20
PRIMER_LEFT_30_TM=57.018
PRIMER_RIGHT_30_TM=59.771
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=940
PRIMER_PAIR_31_PENALTY=3.235853
PRIMER_LEFT_31_PENALTY=1.445265
PRIMER_RIGHT_31_PENALTY=1.790588
PRIMER_LEFT_31_SEQUENCE=CCTTTGCATATTTGCCCTTCT
PRIMER_RIGHT_31_SEQUENCE=CTACATACACTGCACCGCACA
PRIMER_LEFT_31=901,21
PRIMER_RIGHT_31=1842,21
PRIMER_LEFT_31_TM=60.445
PRIMER_RIGHT_31_TM=60.791
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=942
PRIMER_PAIR_32_PENALTY=3.292227
PRIMER_LEFT_32_PENALTY=2.095324
PRIMER_RIGHT_32_PENALTY=1.196903
PRIMER_LEFT_32_SEQUENCE=CTTTGCATATTTGCCCTTCTTC
PRIMER_RIGHT_32_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_32=902,22
PRIMER_RIGHT_32=1843,21
PRIMER_LEFT_32_TM=60.095
PRIMER_RIGHT_32_TM=59.803
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=942
PRIMER_PAIR_33_PENALTY=3.308577
PRIMER_LEFT_33_PENALTY=2.095324
PRIMER_RIGHT_33_PENALTY=1.213253
PRIMER_LEFT_33_SEQUENCE=CTTTGCATATTTGCCCTTCTTC
PRIMER_RIGHT_33_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_33=902,22
PRIMER_RIGHT_33=1840,20
PRIMER_LEFT_33_TM=60.095
PRIMER_RIGHT_33_TM=61.213
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=939
PRIMER_PAIR_34_PENALTY=3.352820
PRIMER_LEFT_34_PENALTY=2.155917
PRIMER_RIGHT_34_PENALTY=1.196903
PRIMER_LEFT_34_SEQUENCE=TTGCATATTTGCCCTTCTTC
PRIMER_RIGHT_34_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_34=904,20
PRIMER_RIGHT_34=1843,21
PRIMER_LEFT_34_TM=57.844
PRIMER_RIGHT_34_TM=59.803
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=9.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=940
PRIMER_PAIR_35_PENALTY=3.369170
PRIMER_LEFT_35_PENALTY=2.155917
PRIMER_RIGHT_35_PENALTY=1.213253
PRIMER_LEFT_35_SEQUENCE=TTGCATATTTGCCCTTCTTC
PRIMER_RIGHT_35_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_35=904,20
PRIMER_RIGHT_35=1840,20
PRIMER_LEFT_35_TM=57.844
PRIMER_RIGHT_35_TM=61.213
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=937
PRIMER_PAIR_36_PENALTY=3.388938
PRIMER_LEFT_36_PENALTY=3.160245
PRIMER_RIGHT_36_PENALTY=0.228693
PRIMER_LEFT_36_SEQUENCE=ACCTTTGCATATTTGCCCTTCT
PRIMER_RIGHT_36_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_36=900,22
PRIMER_RIGHT_36=1841,20
PRIMER_LEFT_36_TM=61.160
PRIMER_RIGHT_36_TM=59.771
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=942
PRIMER_PAIR_37_PENALTY=3.414306
PRIMER_LEFT_37_PENALTY=1.325871
PRIMER_RIGHT_37_PENALTY=2.088435
PRIMER_LEFT_37_SEQUENCE=ACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_37_SEQUENCE=ATCTACATACACTGCACCGCAC
PRIMER_LEFT_37=900,21
PRIMER_RIGHT_37=1844,22
PRIMER_LEFT_37_TM=60.326
PRIMER_RIGHT_37_TM=60.088
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=945
PRIMER_PAIR_38_PENALTY=3.420917
PRIMER_LEFT_38_PENALTY=2.224014
PRIMER_RIGHT_38_PENALTY=1.196903
PRIMER_LEFT_38_SEQUENCE=TGCATATTTGCCCTTCTTCTCT
PRIMER_RIGHT_38_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_38=905,22
PRIMER_RIGHT_38=1843,21
PRIMER_LEFT_38_TM=60.224
PRIMER_RIGHT_38_TM=59.803
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=9.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=939
PRIMER_PAIR_39_PENALTY=3.437267
PRIMER_LEFT_39_PENALTY=2.224014
PRIMER_RIGHT_39_PENALTY=1.213253
PRIMER_LEFT_39_SEQUENCE=TGCATATTTGCCCTTCTTCTCT
PRIMER_RIGHT_39_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_39=905,22
PRIMER_RIGHT_39=1840,20
PRIMER_LEFT_39_TM=60.224
PRIMER_RIGHT_39_TM=61.213
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=936
PRIMER_PAIR_40_PENALTY=3.459972
PRIMER_LEFT_40_PENALTY=3.231279
PRIMER_RIGHT_40_PENALTY=0.228693
PRIMER_LEFT_40_SEQUENCE=GCATATTTGCCCTTCTTCTCTCT
PRIMER_RIGHT_40_SEQUENCE=TACATACACTGCACCGCACA
PRIMER_LEFT_40=906,23
PRIMER_RIGHT_40=1841,20
PRIMER_LEFT_40_TM=60.231
PRIMER_RIGHT_40_TM=59.771
PRIMER_LEFT_40_GC_PERCENT=43.478
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=936
PRIMER_PAIR_41_PENALTY=3.480456
PRIMER_LEFT_41_PENALTY=1.689868
PRIMER_RIGHT_41_PENALTY=1.790588
PRIMER_LEFT_41_SEQUENCE=TGCATATTTGCCCTTCTTCTC
PRIMER_RIGHT_41_SEQUENCE=CTACATACACTGCACCGCACA
PRIMER_LEFT_41=905,21
PRIMER_RIGHT_41=1842,21
PRIMER_LEFT_41_TM=59.310
PRIMER_RIGHT_41_TM=60.791
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=938
PRIMER_PAIR_42_PENALTY=3.501775
PRIMER_LEFT_42_PENALTY=1.325871
PRIMER_RIGHT_42_PENALTY=2.175904
PRIMER_LEFT_42_SEQUENCE=ACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_42_SEQUENCE=TACATACACTGCACCGCACAA
PRIMER_LEFT_42=900,21
PRIMER_RIGHT_42=1841,21
PRIMER_LEFT_42_TM=60.326
PRIMER_RIGHT_42_TM=61.176
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=942
PRIMER_PAIR_43_PENALTY=3.506537
PRIMER_LEFT_43_PENALTY=1.418101
PRIMER_RIGHT_43_PENALTY=2.088435
PRIMER_LEFT_43_SEQUENCE=ACCTTTGCATATTTGCCCTT
PRIMER_RIGHT_43_SEQUENCE=ATCTACATACACTGCACCGCAC
PRIMER_LEFT_43=900,20
PRIMER_RIGHT_43=1844,22
PRIMER_LEFT_43_TM=58.582
PRIMER_RIGHT_43_TM=60.088
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=9.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=945
PRIMER_PAIR_44_PENALTY=3.521287
PRIMER_LEFT_44_PENALTY=2.324383
PRIMER_RIGHT_44_PENALTY=1.196903
PRIMER_LEFT_44_SEQUENCE=TACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_44_SEQUENCE=TCTACATACACTGCACCGCAC
PRIMER_LEFT_44=899,22
PRIMER_RIGHT_44=1843,21
PRIMER_LEFT_44_TM=60.324
PRIMER_RIGHT_44_TM=59.803
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=945
PRIMER_PAIR_45_PENALTY=3.531305
PRIMER_LEFT_45_PENALTY=1.325871
PRIMER_RIGHT_45_PENALTY=2.205434
PRIMER_LEFT_45_SEQUENCE=ACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_45_SEQUENCE=CTACATACACTGCACCGCAC
PRIMER_LEFT_45=900,21
PRIMER_RIGHT_45=1842,20
PRIMER_LEFT_45_TM=60.326
PRIMER_RIGHT_45_TM=57.795
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=943
PRIMER_PAIR_46_PENALTY=3.533701
PRIMER_LEFT_46_PENALTY=1.445265
PRIMER_RIGHT_46_PENALTY=2.088435
PRIMER_LEFT_46_SEQUENCE=CCTTTGCATATTTGCCCTTCT
PRIMER_RIGHT_46_SEQUENCE=ATCTACATACACTGCACCGCAC
PRIMER_LEFT_46=901,21
PRIMER_RIGHT_46=1844,22
PRIMER_LEFT_46_TM=60.445
PRIMER_RIGHT_46_TM=60.088
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=944
PRIMER_PAIR_47_PENALTY=3.537637
PRIMER_LEFT_47_PENALTY=2.324383
PRIMER_RIGHT_47_PENALTY=1.213253
PRIMER_LEFT_47_SEQUENCE=TACCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_47_SEQUENCE=ACATACACTGCACCGCACAA
PRIMER_LEFT_47=899,22
PRIMER_RIGHT_47=1840,20
PRIMER_LEFT_47_TM=60.324
PRIMER_RIGHT_47_TM=61.213
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=942
PRIMER_PAIR_48_PENALTY=3.548578
PRIMER_LEFT_48_PENALTY=0.453004
PRIMER_RIGHT_48_PENALTY=3.095574
PRIMER_LEFT_48_SEQUENCE=CCTTTGCATATTTGCCCTTC
PRIMER_RIGHT_48_SEQUENCE=TATCTACATACACTGCACCGCAC
PRIMER_LEFT_48=901,20
PRIMER_RIGHT_48=1845,23
PRIMER_LEFT_48_TM=59.547
PRIMER_RIGHT_48_TM=60.096
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=47.826
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=945
PRIMER_PAIR_49_PENALTY=3.594006
PRIMER_LEFT_49_PENALTY=1.418101
PRIMER_RIGHT_49_PENALTY=2.175904
PRIMER_LEFT_49_SEQUENCE=ACCTTTGCATATTTGCCCTT
PRIMER_RIGHT_49_SEQUENCE=TACATACACTGCACCGCACAA
PRIMER_LEFT_49=900,20
PRIMER_RIGHT_49=1841,21
PRIMER_LEFT_49_TM=58.582
PRIMER_RIGHT_49_TM=61.176
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=942
=
SEQUENCE_ID=MAGIC.329_2_5839019_5839443_col-0_zu-0_5829429_93736_TRUE
SEQUENCE_TARGET=950,524
SEQUENCE_TEMPLATE=AGCTGTAAGCTNCCTTTANTCCCTCTCTTTTGGTGCAACAATAAAAAACCTCATCTTTTCGATTTCATTTGCCGTGCNTGCACAACCNTAGAGCNAGNCACAAGCTATTACGTTTGTGTCNCATGTGGTGACCAGTTNCACAAAGAATGTATTNGTTCTCCACTTGAGTTCAAACACCCTTCATATCCNTCTCTTTCTCTCCAACTTTACAGTCCTNCNTCCGACCNCGTATNCTGCNCATGTTGTCAAAAACGTATCGATGGCNTGANTTATTATNGTCCTACCAACAANTNNNNNNNNNNTCTNTNNTGTGCATTCAAACCAATACCCTNTNTTATAGATCACCCNAAAAGGCATCCCCATCCTCTCACNNTTTTCCCCAAACANTCTNTCNTNCCTTGCNATGTTTGTAGCCTCATNAAAGNACATATTCCNACNTATGTNTGTGTTAGATGTGTNTTTGTAGTTCATCAAGATTGTATCTATTTCCCGTATGTCNTNAANATATNCCGTCACCACCACNGTATATCTTANACCTCCTCTCTTCCATCTGGAANATGGTCTTGCGGAGTCTGTCNTCAAATGGTNGACAANGACTATGGTGCCTACTCTTGCAACAAGTGTGATGACTATTTTGTTCATTCAAGATGCGCGNTGCGGAGAGACTTATGGGATGGGATAGAACTCGAGGGAGTNNNNNNNNNACCTGAGATAGTTGTTGAGCCATTTNTCACGATTTCTNATGGTATANTACTCCATNTTTCTCATGGCCATCATCTGAAACTCAAGATCAGTAGAGTCTACGACGAAAAAATNGTTTGTCAAGCGTGNANCCTTCCGATCTACGAGGGNGNCTATTATTCATGTATNGATNAATGTGACTTCATCCTCCACGNAACATGTGCNAATGCNCCTNGCAAGAAACATCATNCNTTGNATNCTCANCCACTTACGCTAANGGTNGTCACTANTGNATATGNNNNNAANATAGGACGTTTTTNTTGTAAGGCTTGTCNNCGTGAAAGTTGTGGTTTCGTCTATGANGNTNTCNGAGGAAAGATTGGTAATTATAGAAGAAATAAATNNCAACTAGATTTACGATGTGCCTCNGTTTCTGAACCATTTGAATATCTAGGCCATAAGCATCTCTTATACCTAGCTTTGAATCNAGAAGAAGANNNNNNNGCAATATGTCAAATCTGCNANNANAGNNNNNNNNNNNNNNNNNNTTGCAGAAAACTAAATTGCNTTGAATGTGATTTTGTGATATGCTTNAGGTGTGCTACCTTNCCATANAAGGCAAGGTATCAACATGANAAGCANTTCCTCAAATTTTATGNGANGAAAGAGGCAAATGATCATTCAGAGTGGTGTGATGCATGCGAAGGAAGAATTGCAGATTTAAGAACGAGAGGGATCTATTCNTGCAACGCAGATTTAAGAACGAAAGGGTTCTATTCATGCGACGACTGCTGCACCACTCTTCATATTGATTGTTTGCTTGGGGAAGACATGTATATGAAGNCTGGTCACACCATAATGTATAATATGANGGGTTTCGACGAACATTATGAGAAGAAGCTTCGTATTCTTCGCAACAATACTTTGTCTCGGCCATNTTGCANTACNTGCGGGNANCGTTGTCGACAGAAAATAGTTTTCAAATACAAAGAACACCATATATTTTGTGCTNTATCTTGCCAGAAGCATGTAATTAATTACTCATAGCCANNCGCTTTGCAATTTTTTAATCTCTCCATATTTCTTCANTAAAACGCATTGTAATCTTGTTTCTTGTCCTCTTCCTATATGTAANTTTGTTATGTTTTTTGATCGATGCTANATGNGCTCTGTTTTNACTTTTTGTTCTTTAAGAANANCATTTTTGATTTCTGTTTTATAATTTTATAATGTTTCTCATCTGTTTNGAAATTTGGAGAACTTCCANNNNTNCTGCCTTTGGTTAGAATTCATAGGCATNTACAGATAATACGAATTTGANAAAAAAAGATGNTTTTGCAGTTTTGTCTATCAAAAGGTCTTGGACTCTATGATNATNTCTTGTTNANGATTCTATGAGTAAGGATGAATCTAGTTCTCATTCAAAGAAATCTGTANAGACNAGTCATTTAAGAGTCCATTAATTCTTGATGGAAATCTATCATTATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.320680
PRIMER_LEFT_0_PENALTY=0.269335
PRIMER_RIGHT_0_PENALTY=0.051345
PRIMER_LEFT_0_SEQUENCE=ATGGTCTTGCGGAGTCTGTC
PRIMER_RIGHT_0_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_0=557,20
PRIMER_RIGHT_0=1510,20
PRIMER_LEFT_0_TM=60.269
PRIMER_RIGHT_0_TM=60.051
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=954
PRIMER_PAIR_1_PENALTY=0.398663
PRIMER_LEFT_1_PENALTY=0.347318
PRIMER_RIGHT_1_PENALTY=0.051345
PRIMER_LEFT_1_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_1_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_1=656,20
PRIMER_RIGHT_1=1510,20
PRIMER_LEFT_1_TM=59.653
PRIMER_RIGHT_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=855
PRIMER_PAIR_2_PENALTY=0.549027
PRIMER_LEFT_2_PENALTY=0.497682
PRIMER_RIGHT_2_PENALTY=0.051345
PRIMER_LEFT_2_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_2_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_2=657,20
PRIMER_RIGHT_2=1510,20
PRIMER_LEFT_2_TM=59.502
PRIMER_RIGHT_2_TM=60.051
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=854
PRIMER_PAIR_3_PENALTY=0.764508
PRIMER_LEFT_3_PENALTY=0.269335
PRIMER_RIGHT_3_PENALTY=0.495173
PRIMER_LEFT_3_SEQUENCE=ATGGTCTTGCGGAGTCTGTC
PRIMER_RIGHT_3_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_3=557,20
PRIMER_RIGHT_3=1514,20
PRIMER_LEFT_3_TM=60.269
PRIMER_RIGHT_3_TM=60.495
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=958
PRIMER_PAIR_4_PENALTY=0.842491
PRIMER_LEFT_4_PENALTY=0.347318
PRIMER_RIGHT_4_PENALTY=0.495173
PRIMER_LEFT_4_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_4_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_4=656,20
PRIMER_RIGHT_4=1514,20
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.495
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=859
PRIMER_PAIR_5_PENALTY=0.842491
PRIMER_LEFT_5_PENALTY=0.347318
PRIMER_RIGHT_5_PENALTY=0.495173
PRIMER_LEFT_5_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_5_SEQUENCE=ACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_5=656,20
PRIMER_RIGHT_5=1515,20
PRIMER_LEFT_5_TM=59.653
PRIMER_RIGHT_5_TM=60.495
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=860
PRIMER_PAIR_6_PENALTY=0.992855
PRIMER_LEFT_6_PENALTY=0.497682
PRIMER_RIGHT_6_PENALTY=0.495173
PRIMER_LEFT_6_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_6_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_6=657,20
PRIMER_RIGHT_6=1514,20
PRIMER_LEFT_6_TM=59.502
PRIMER_RIGHT_6_TM=60.495
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=858
PRIMER_PAIR_7_PENALTY=0.992855
PRIMER_LEFT_7_PENALTY=0.497682
PRIMER_RIGHT_7_PENALTY=0.495173
PRIMER_LEFT_7_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_7_SEQUENCE=ACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_7=657,20
PRIMER_RIGHT_7=1515,20
PRIMER_LEFT_7_TM=59.502
PRIMER_RIGHT_7_TM=60.495
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=859
PRIMER_PAIR_8_PENALTY=1.224354
PRIMER_LEFT_8_PENALTY=0.347318
PRIMER_RIGHT_8_PENALTY=0.877035
PRIMER_LEFT_8_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_8_SEQUENCE=TACATGTCTTCCCCAAGCAA
PRIMER_LEFT_8=656,20
PRIMER_RIGHT_8=1516,20
PRIMER_LEFT_8_TM=59.653
PRIMER_RIGHT_8_TM=59.123
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=861
PRIMER_PAIR_9_PENALTY=1.270136
PRIMER_LEFT_9_PENALTY=0.269335
PRIMER_RIGHT_9_PENALTY=1.000801
PRIMER_LEFT_9_SEQUENCE=ATGGTCTTGCGGAGTCTGTC
PRIMER_RIGHT_9_SEQUENCE=CTTCCCCAAGCAAACAATCA
PRIMER_LEFT_9=557,20
PRIMER_RIGHT_9=1509,20
PRIMER_LEFT_9_TM=60.269
PRIMER_RIGHT_9_TM=61.001
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=953
PRIMER_PAIR_10_PENALTY=1.329674
PRIMER_LEFT_10_PENALTY=1.278329
PRIMER_RIGHT_10_PENALTY=0.051345
PRIMER_LEFT_10_SEQUENCE=GGAGAGACTTATGGGATGGGA
PRIMER_RIGHT_10_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_10=658,21
PRIMER_RIGHT_10=1510,20
PRIMER_LEFT_10_TM=60.278
PRIMER_RIGHT_10_TM=60.051
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=853
PRIMER_PAIR_11_PENALTY=1.348119
PRIMER_LEFT_11_PENALTY=0.347318
PRIMER_RIGHT_11_PENALTY=1.000801
PRIMER_LEFT_11_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_11_SEQUENCE=CTTCCCCAAGCAAACAATCA
PRIMER_LEFT_11=656,20
PRIMER_RIGHT_11=1509,20
PRIMER_LEFT_11_TM=59.653
PRIMER_RIGHT_11_TM=61.001
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=854
PRIMER_PAIR_12_PENALTY=1.374718
PRIMER_LEFT_12_PENALTY=0.497682
PRIMER_RIGHT_12_PENALTY=0.877035
PRIMER_LEFT_12_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_12_SEQUENCE=TACATGTCTTCCCCAAGCAA
PRIMER_LEFT_12=657,20
PRIMER_RIGHT_12=1516,20
PRIMER_LEFT_12_TM=59.502
PRIMER_RIGHT_12_TM=59.123
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=860
PRIMER_PAIR_13_PENALTY=1.406751
PRIMER_LEFT_13_PENALTY=1.355407
PRIMER_RIGHT_13_PENALTY=0.051345
PRIMER_LEFT_13_SEQUENCE=GGCCATCATCTGAAACTCAA
PRIMER_RIGHT_13_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_13=768,20
PRIMER_RIGHT_13=1510,20
PRIMER_LEFT_13_TM=58.645
PRIMER_RIGHT_13_TM=60.051
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=743
PRIMER_PAIR_14_PENALTY=1.467126
PRIMER_LEFT_14_PENALTY=1.415781
PRIMER_RIGHT_14_PENALTY=0.051345
PRIMER_LEFT_14_SEQUENCE=GATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_14_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_14=672,21
PRIMER_RIGHT_14=1510,20
PRIMER_LEFT_14_TM=60.416
PRIMER_RIGHT_14_TM=60.051
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=839
PRIMER_PAIR_15_PENALTY=1.498483
PRIMER_LEFT_15_PENALTY=0.497682
PRIMER_RIGHT_15_PENALTY=1.000801
PRIMER_LEFT_15_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_15_SEQUENCE=CTTCCCCAAGCAAACAATCA
PRIMER_LEFT_15=657,20
PRIMER_RIGHT_15=1509,20
PRIMER_LEFT_15_TM=59.502
PRIMER_RIGHT_15_TM=61.001
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=853
PRIMER_PAIR_16_PENALTY=1.508538
PRIMER_LEFT_16_PENALTY=1.457193
PRIMER_RIGHT_16_PENALTY=0.051345
PRIMER_LEFT_16_SEQUENCE=ATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_16_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_16=673,20
PRIMER_RIGHT_16=1510,20
PRIMER_LEFT_16_TM=58.543
PRIMER_RIGHT_16_TM=60.051
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=838
PRIMER_PAIR_17_PENALTY=1.639052
PRIMER_LEFT_17_PENALTY=0.269335
PRIMER_RIGHT_17_PENALTY=1.369717
PRIMER_LEFT_17_SEQUENCE=ATGGTCTTGCGGAGTCTGTC
PRIMER_RIGHT_17_SEQUENCE=TTCCCCAAGCAAACAATCAA
PRIMER_LEFT_17=557,20
PRIMER_RIGHT_17=1508,20
PRIMER_LEFT_17_TM=60.269
PRIMER_RIGHT_17_TM=61.370
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=952
PRIMER_PAIR_18_PENALTY=1.658198
PRIMER_LEFT_18_PENALTY=1.606853
PRIMER_RIGHT_18_PENALTY=0.051345
PRIMER_LEFT_18_SEQUENCE=GGAGAGACTTATGGGATGGG
PRIMER_RIGHT_18_SEQUENCE=TCTTCCCCAAGCAAACAATC
PRIMER_LEFT_18=658,20
PRIMER_RIGHT_18=1510,20
PRIMER_LEFT_18_TM=58.393
PRIMER_RIGHT_18_TM=60.051
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.6000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=853
PRIMER_PAIR_19_PENALTY=1.717035
PRIMER_LEFT_19_PENALTY=0.347318
PRIMER_RIGHT_19_PENALTY=1.369717
PRIMER_LEFT_19_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_19_SEQUENCE=TTCCCCAAGCAAACAATCAA
PRIMER_LEFT_19=656,20
PRIMER_RIGHT_19=1508,20
PRIMER_LEFT_19_TM=59.653
PRIMER_RIGHT_19_TM=61.370
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=853
PRIMER_PAIR_20_PENALTY=1.756431
PRIMER_LEFT_20_PENALTY=0.269335
PRIMER_RIGHT_20_PENALTY=1.487097
PRIMER_LEFT_20_SEQUENCE=ATGGTCTTGCGGAGTCTGTC
PRIMER_RIGHT_20_SEQUENCE=TACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_20=557,20
PRIMER_RIGHT_20=1516,21
PRIMER_LEFT_20_TM=60.269
PRIMER_RIGHT_20_TM=60.487
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.8000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=960
PRIMER_PAIR_21_PENALTY=1.773502
PRIMER_LEFT_21_PENALTY=1.278329
PRIMER_RIGHT_21_PENALTY=0.495173
PRIMER_LEFT_21_SEQUENCE=GGAGAGACTTATGGGATGGGA
PRIMER_RIGHT_21_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_21=658,21
PRIMER_RIGHT_21=1514,20
PRIMER_LEFT_21_TM=60.278
PRIMER_RIGHT_21_TM=60.495
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=857
PRIMER_PAIR_22_PENALTY=1.773502
PRIMER_LEFT_22_PENALTY=1.278329
PRIMER_RIGHT_22_PENALTY=0.495173
PRIMER_LEFT_22_SEQUENCE=GGAGAGACTTATGGGATGGGA
PRIMER_RIGHT_22_SEQUENCE=ACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_22=658,21
PRIMER_RIGHT_22=1515,20
PRIMER_LEFT_22_TM=60.278
PRIMER_RIGHT_22_TM=60.495
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=8.8000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=858
PRIMER_PAIR_23_PENALTY=1.834415
PRIMER_LEFT_23_PENALTY=0.347318
PRIMER_RIGHT_23_PENALTY=1.487097
PRIMER_LEFT_23_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_23_SEQUENCE=TACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_23=656,20
PRIMER_RIGHT_23=1516,21
PRIMER_LEFT_23_TM=59.653
PRIMER_RIGHT_23_TM=60.487
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=861
PRIMER_PAIR_24_PENALTY=1.849076
PRIMER_LEFT_24_PENALTY=0.269335
PRIMER_RIGHT_24_PENALTY=1.579741
PRIMER_LEFT_24_SEQUENCE=ATGGTCTTGCGGAGTCTGTC
PRIMER_RIGHT_24_SEQUENCE=TGTCTTCCCCAAGCAAACAA
PRIMER_LEFT_24=557,20
PRIMER_RIGHT_24=1512,20
PRIMER_LEFT_24_TM=60.269
PRIMER_RIGHT_24_TM=61.580
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=956
PRIMER_PAIR_25_PENALTY=1.850579
PRIMER_LEFT_25_PENALTY=1.355407
PRIMER_RIGHT_25_PENALTY=0.495173
PRIMER_LEFT_25_SEQUENCE=GGCCATCATCTGAAACTCAA
PRIMER_RIGHT_25_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_25=768,20
PRIMER_RIGHT_25=1514,20
PRIMER_LEFT_25_TM=58.645
PRIMER_RIGHT_25_TM=60.495
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=747
PRIMER_PAIR_26_PENALTY=1.850579
PRIMER_LEFT_26_PENALTY=1.355407
PRIMER_RIGHT_26_PENALTY=0.495173
PRIMER_LEFT_26_SEQUENCE=GGCCATCATCTGAAACTCAA
PRIMER_RIGHT_26_SEQUENCE=ACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_26=768,20
PRIMER_RIGHT_26=1515,20
PRIMER_LEFT_26_TM=58.645
PRIMER_RIGHT_26_TM=60.495
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=748
PRIMER_PAIR_27_PENALTY=1.867399
PRIMER_LEFT_27_PENALTY=0.497682
PRIMER_RIGHT_27_PENALTY=1.369717
PRIMER_LEFT_27_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_27_SEQUENCE=TTCCCCAAGCAAACAATCAA
PRIMER_LEFT_27=657,20
PRIMER_RIGHT_27=1508,20
PRIMER_LEFT_27_TM=59.502
PRIMER_RIGHT_27_TM=61.370
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=852
PRIMER_PAIR_28_PENALTY=1.904087
PRIMER_LEFT_28_PENALTY=0.347318
PRIMER_RIGHT_28_PENALTY=1.556768
PRIMER_LEFT_28_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_28_SEQUENCE=ATACATGTCTTCCCCAAGCAA
PRIMER_LEFT_28=656,20
PRIMER_RIGHT_28=1517,21
PRIMER_LEFT_28_TM=59.653
PRIMER_RIGHT_28_TM=59.443
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.1000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=862
PRIMER_PAIR_29_PENALTY=1.910954
PRIMER_LEFT_29_PENALTY=1.415781
PRIMER_RIGHT_29_PENALTY=0.495173
PRIMER_LEFT_29_SEQUENCE=GATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_29_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_29=672,21
PRIMER_RIGHT_29=1514,20
PRIMER_LEFT_29_TM=60.416
PRIMER_RIGHT_29_TM=60.495
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=843
PRIMER_PAIR_30_PENALTY=1.910954
PRIMER_LEFT_30_PENALTY=1.415781
PRIMER_RIGHT_30_PENALTY=0.495173
PRIMER_LEFT_30_SEQUENCE=GATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_30_SEQUENCE=ACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_30=672,21
PRIMER_RIGHT_30=1515,20
PRIMER_LEFT_30_TM=60.416
PRIMER_RIGHT_30_TM=60.495
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=8.8000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=844
PRIMER_PAIR_31_PENALTY=1.927060
PRIMER_LEFT_31_PENALTY=0.347318
PRIMER_RIGHT_31_PENALTY=1.579741
PRIMER_LEFT_31_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_31_SEQUENCE=TGTCTTCCCCAAGCAAACAA
PRIMER_LEFT_31=656,20
PRIMER_RIGHT_31=1512,20
PRIMER_LEFT_31_TM=59.653
PRIMER_RIGHT_31_TM=61.580
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=857
PRIMER_PAIR_32_PENALTY=1.952365
PRIMER_LEFT_32_PENALTY=1.457193
PRIMER_RIGHT_32_PENALTY=0.495173
PRIMER_LEFT_32_SEQUENCE=ATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_32_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_32=673,20
PRIMER_RIGHT_32=1514,20
PRIMER_LEFT_32_TM=58.543
PRIMER_RIGHT_32_TM=60.495
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=842
PRIMER_PAIR_33_PENALTY=1.952365
PRIMER_LEFT_33_PENALTY=1.457193
PRIMER_RIGHT_33_PENALTY=0.495173
PRIMER_LEFT_33_SEQUENCE=ATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_33_SEQUENCE=ACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_33=673,20
PRIMER_RIGHT_33=1515,20
PRIMER_LEFT_33_TM=58.543
PRIMER_RIGHT_33_TM=60.495
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=8.8000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=843
PRIMER_PAIR_34_PENALTY=1.984779
PRIMER_LEFT_34_PENALTY=0.497682
PRIMER_RIGHT_34_PENALTY=1.487097
PRIMER_LEFT_34_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_34_SEQUENCE=TACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_34=657,20
PRIMER_RIGHT_34=1516,21
PRIMER_LEFT_34_TM=59.502
PRIMER_RIGHT_34_TM=60.487
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=8.8000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=860
PRIMER_PAIR_35_PENALTY=2.054451
PRIMER_LEFT_35_PENALTY=0.497682
PRIMER_RIGHT_35_PENALTY=1.556768
PRIMER_LEFT_35_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_35_SEQUENCE=ATACATGTCTTCCCCAAGCAA
PRIMER_LEFT_35=657,20
PRIMER_RIGHT_35=1517,21
PRIMER_LEFT_35_TM=59.502
PRIMER_RIGHT_35_TM=59.443
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=861
PRIMER_PAIR_36_PENALTY=2.077424
PRIMER_LEFT_36_PENALTY=0.497682
PRIMER_RIGHT_36_PENALTY=1.579741
PRIMER_LEFT_36_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_36_SEQUENCE=TGTCTTCCCCAAGCAAACAA
PRIMER_LEFT_36=657,20
PRIMER_RIGHT_36=1512,20
PRIMER_LEFT_36_TM=59.502
PRIMER_RIGHT_36_TM=61.580
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=856
PRIMER_PAIR_37_PENALTY=2.102025
PRIMER_LEFT_37_PENALTY=1.606853
PRIMER_RIGHT_37_PENALTY=0.495173
PRIMER_LEFT_37_SEQUENCE=GGAGAGACTTATGGGATGGG
PRIMER_RIGHT_37_SEQUENCE=CATGTCTTCCCCAAGCAAAC
PRIMER_LEFT_37=658,20
PRIMER_RIGHT_37=1514,20
PRIMER_LEFT_37_TM=58.393
PRIMER_RIGHT_37_TM=60.495
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=857
PRIMER_PAIR_38_PENALTY=2.102025
PRIMER_LEFT_38_PENALTY=1.606853
PRIMER_RIGHT_38_PENALTY=0.495173
PRIMER_LEFT_38_SEQUENCE=GGAGAGACTTATGGGATGGG
PRIMER_RIGHT_38_SEQUENCE=ACATGTCTTCCCCAAGCAAA
PRIMER_LEFT_38=658,20
PRIMER_RIGHT_38=1515,20
PRIMER_LEFT_38_TM=58.393
PRIMER_RIGHT_38_TM=60.495
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=8.8000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=858
PRIMER_PAIR_39_PENALTY=2.120194
PRIMER_LEFT_39_PENALTY=0.269335
PRIMER_RIGHT_39_PENALTY=1.850859
PRIMER_LEFT_39_SEQUENCE=ATGGTCTTGCGGAGTCTGTC
PRIMER_RIGHT_39_SEQUENCE=GTCTTCCCCAAGCAAACAATC
PRIMER_LEFT_39=557,20
PRIMER_RIGHT_39=1511,21
PRIMER_LEFT_39_TM=60.269
PRIMER_RIGHT_39_TM=60.851
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=955
PRIMER_PAIR_40_PENALTY=2.155365
PRIMER_LEFT_40_PENALTY=1.278329
PRIMER_RIGHT_40_PENALTY=0.877035
PRIMER_LEFT_40_SEQUENCE=GGAGAGACTTATGGGATGGGA
PRIMER_RIGHT_40_SEQUENCE=TACATGTCTTCCCCAAGCAA
PRIMER_LEFT_40=658,21
PRIMER_RIGHT_40=1516,20
PRIMER_LEFT_40_TM=60.278
PRIMER_RIGHT_40_TM=59.123
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=859
PRIMER_PAIR_41_PENALTY=2.198178
PRIMER_LEFT_41_PENALTY=0.347318
PRIMER_RIGHT_41_PENALTY=1.850859
PRIMER_LEFT_41_SEQUENCE=GCGGAGAGACTTATGGGATG
PRIMER_RIGHT_41_SEQUENCE=GTCTTCCCCAAGCAAACAATC
PRIMER_LEFT_41=656,20
PRIMER_RIGHT_41=1511,21
PRIMER_LEFT_41_TM=59.653
PRIMER_RIGHT_41_TM=60.851
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=856
PRIMER_PAIR_42_PENALTY=2.232442
PRIMER_LEFT_42_PENALTY=1.355407
PRIMER_RIGHT_42_PENALTY=0.877035
PRIMER_LEFT_42_SEQUENCE=GGCCATCATCTGAAACTCAA
PRIMER_RIGHT_42_SEQUENCE=TACATGTCTTCCCCAAGCAA
PRIMER_LEFT_42=768,20
PRIMER_RIGHT_42=1516,20
PRIMER_LEFT_42_TM=58.645
PRIMER_RIGHT_42_TM=59.123
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=749
PRIMER_PAIR_43_PENALTY=2.279131
PRIMER_LEFT_43_PENALTY=1.278329
PRIMER_RIGHT_43_PENALTY=1.000801
PRIMER_LEFT_43_SEQUENCE=GGAGAGACTTATGGGATGGGA
PRIMER_RIGHT_43_SEQUENCE=CTTCCCCAAGCAAACAATCA
PRIMER_LEFT_43=658,21
PRIMER_RIGHT_43=1509,20
PRIMER_LEFT_43_TM=60.278
PRIMER_RIGHT_43_TM=61.001
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=852
PRIMER_PAIR_44_PENALTY=2.292817
PRIMER_LEFT_44_PENALTY=1.415781
PRIMER_RIGHT_44_PENALTY=0.877035
PRIMER_LEFT_44_SEQUENCE=GATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_44_SEQUENCE=TACATGTCTTCCCCAAGCAA
PRIMER_LEFT_44=672,21
PRIMER_RIGHT_44=1516,20
PRIMER_LEFT_44_TM=60.416
PRIMER_RIGHT_44_TM=59.123
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=845
PRIMER_PAIR_45_PENALTY=2.334228
PRIMER_LEFT_45_PENALTY=1.457193
PRIMER_RIGHT_45_PENALTY=0.877035
PRIMER_LEFT_45_SEQUENCE=ATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_45_SEQUENCE=TACATGTCTTCCCCAAGCAA
PRIMER_LEFT_45=673,20
PRIMER_RIGHT_45=1516,20
PRIMER_LEFT_45_TM=58.543
PRIMER_RIGHT_45_TM=59.123
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=844
PRIMER_PAIR_46_PENALTY=2.348542
PRIMER_LEFT_46_PENALTY=0.497682
PRIMER_RIGHT_46_PENALTY=1.850859
PRIMER_LEFT_46_SEQUENCE=CGGAGAGACTTATGGGATGG
PRIMER_RIGHT_46_SEQUENCE=GTCTTCCCCAAGCAAACAATC
PRIMER_LEFT_46=657,20
PRIMER_RIGHT_46=1511,21
PRIMER_LEFT_46_TM=59.502
PRIMER_RIGHT_46_TM=60.851
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.1000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=855
PRIMER_PAIR_47_PENALTY=2.356208
PRIMER_LEFT_47_PENALTY=1.355407
PRIMER_RIGHT_47_PENALTY=1.000801
PRIMER_LEFT_47_SEQUENCE=GGCCATCATCTGAAACTCAA
PRIMER_RIGHT_47_SEQUENCE=CTTCCCCAAGCAAACAATCA
PRIMER_LEFT_47=768,20
PRIMER_RIGHT_47=1509,20
PRIMER_LEFT_47_TM=58.645
PRIMER_RIGHT_47_TM=61.001
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=742
PRIMER_PAIR_48_PENALTY=2.416582
PRIMER_LEFT_48_PENALTY=1.415781
PRIMER_RIGHT_48_PENALTY=1.000801
PRIMER_LEFT_48_SEQUENCE=GATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_48_SEQUENCE=CTTCCCCAAGCAAACAATCA
PRIMER_LEFT_48=672,21
PRIMER_RIGHT_48=1509,20
PRIMER_LEFT_48_TM=60.416
PRIMER_RIGHT_48_TM=61.001
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=838
PRIMER_PAIR_49_PENALTY=2.457994
PRIMER_LEFT_49_PENALTY=1.457193
PRIMER_RIGHT_49_PENALTY=1.000801
PRIMER_LEFT_49_SEQUENCE=ATGGGATAGAACTCGAGGGA
PRIMER_RIGHT_49_SEQUENCE=CTTCCCCAAGCAAACAATCA
PRIMER_LEFT_49=673,20
PRIMER_RIGHT_49=1509,20
PRIMER_LEFT_49_TM=58.543
PRIMER_RIGHT_49_TM=61.001
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=837
=
SEQUENCE_ID=MAGIC.446_2_5915965_5916282_kn-0_tsu-0_6514_4266_TRUE
SEQUENCE_TARGET=950,417
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNGAGGAGGCAATCNGATAACAACGACCAAACTTGGAANCAATCTTTGGATAAAGTNTNGGAAACNTTTCTTNCCACGAACNTTAGAACNATNTGTCCATTATNCACTANTTCTTCNGAACGCATTNTTAANAACNNNGNNAAATCNNTCTTGAACTGATTTAANTANCTCTNGTAGANATTTGNAGGGTANGNATNTTTAATATGAATGCTTTTNGTNTTGCTCTCAAGNCCATCTGGAATTNTAGAAAGCCAATGGANACTAAAAGCTGAATGAACAAAATGGAGAGACTTTCTTGGGAATANCCTTGAGTAAAAAGAACCCGGGACNCCCGAGACAAANCACTTTCCTTTAACNTCCGGTTTCAGCTTCTCTTGGAAAGAATGAAACANCTTGAAGGTGGTATTGAAATCGTTNTCTGGAAGNTCGTTCAAATAACAATCGATNTCTGGTNGGTTTTTACTCNTTTGTTGGTATGACTCCATGATCGTGTANGCGATTGCGGACATGGCCAACACCGTGTTTTGTCCAGTAGAACAACCCAAGTCTGCCACTTTGATGCAACCAGGAAACCNATTTTTCAGTAGCATTTCTCTTACGTTCTCTATGACCAAAGGTTGTATCTCATAGAAAACACTTCTCTATAAAACNCCAAAAATATCATANATTGTCANACCTTGATTTANNAGNACNTAGTTATTATATCATCACATTCGTNAGTNTTTANNTACCTGATAATGTGAATTNGTAGCATAACTATGGTCTCCTGTTCCTCCTTTCATNNTAAGAGCGTCCACCAAAGAGTCCCTNACGTATATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCATAAACACNNNNNNNNNNNNNNNNNNNNNNNNNNNACCTTAGNATTAGAAGCTTTAGTATANCAACAAAANGATGATTACAATCTAATCTACCCATTTNAGCTCTAAANAAAGANTTCTNNNNAACAANGAAANNNAAATTATACAAGTACAAAGTTACAATTACAAGAAATNAATCATCCCATTTACTTAAAATNTCGAAGAATTCAANNATTTGTTTGNATGAATGTAANATTTATTTATAGNAAAANNNGGTTTTACAATTAATGCAACATANAGNCGAAGATCAAAAAACATTTTAGAAGAAATCAATCATTACCTTGAGGGAGACATGATGTTCACAATATGTATGTATCAATTTTCTTTACCAAATGTATAAGGTCTANGATTAGAAGATCTTGNCCTTGAACTAGCTTGTATGCATGNGTACNTNAAANAAAGGNCACTTTAGTTTTTCTGGTAATTTTTTCNGATGGTANNTGTGTNCTGGAAACGAGNACTGTCGCATACTTAAGAGCCACTTGNATCAAGCACANACACACACACACANANCACTTGTATATCTTACACCTAATANNNTTATATTTGCATTTATTTTAATTTTACAACAGATCAAATTGGNAAATATNNAAAANNNNNCAAGAAAATGGGNAACATGATAACGTAATTAAAATAGTNCNGAGCTNTTCNNTTTNTTNTTTTTNATGTATGTAATNAAATAAGNNNNTTNTTTATTTTGGATTTAAATGTACATTTTCNGTCGNTTTTNNNNNNNNNNNNNNNNTTCTTATTTATTATNGANCNATTATNAAAAACTANAATAAAGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAGTNGAGGCNCGTCNCCAACCANTCGACCATCAGACAATGAAGANATGCCTGTTTNTGATTTNTTTNAGATAANAAAACTTCTGACTTAGTTTTCNGCTANGATNGGATNTAGTTTNTTTGTANGAGGANTAAATCTTNGGTCCNANANCTTTTNNNTANNNNNNNNNNNAAANNCATNCTTTAACCTANNNNNNAAATAGANATATTATATATACANATATGAACATNTTNTTATTGTAATTATGGAATGCAATATTTTNATGCAANGATCANATCTAATAACTTAAANGAGNTTCATANNNNNANAANAANNNNNNNNAACNATTTCACACANTGTAGCATTCATNANTTCTNAACAATACTTAGCCTCATGAGTTTATGATGTGCANCAATCATTATTATTNTTGTGGCCAAAGNAACCTCTATNTCCCTTGCCTCTNTNTGGTTCATCGANTNTTTCTTATNCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.094906
PRIMER_LEFT_0_PENALTY=0.308722
PRIMER_RIGHT_0_PENALTY=2.786184
PRIMER_LEFT_0_SEQUENCE=CACTTTGATGCAACCAGGAA
PRIMER_RIGHT_0_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_0=572,20
PRIMER_RIGHT_0=1404,21
PRIMER_LEFT_0_TM=59.691
PRIMER_RIGHT_0_TM=58.214
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=833
PRIMER_PAIR_1_PENALTY=3.340949
PRIMER_LEFT_1_PENALTY=0.554765
PRIMER_RIGHT_1_PENALTY=2.786184
PRIMER_LEFT_1_SEQUENCE=GAACAACCCAAGTCTGCCAC
PRIMER_RIGHT_1_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_1=555,20
PRIMER_RIGHT_1=1404,21
PRIMER_LEFT_1_TM=60.555
PRIMER_RIGHT_1_TM=58.214
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=850
PRIMER_PAIR_2_PENALTY=3.498052
PRIMER_LEFT_2_PENALTY=0.711868
PRIMER_RIGHT_2_PENALTY=2.786184
PRIMER_LEFT_2_SEQUENCE=CCAAGTCTGCCACTTTGATG
PRIMER_RIGHT_2_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_2=562,20
PRIMER_RIGHT_2=1404,21
PRIMER_LEFT_2_TM=59.288
PRIMER_RIGHT_2_TM=58.214
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=843
PRIMER_PAIR_3_PENALTY=3.659754
PRIMER_LEFT_3_PENALTY=0.873570
PRIMER_RIGHT_3_PENALTY=2.786184
PRIMER_LEFT_3_SEQUENCE=TTTGATGCAACCAGGAAACC
PRIMER_RIGHT_3_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_3=575,20
PRIMER_RIGHT_3=1404,21
PRIMER_LEFT_3_TM=60.874
PRIMER_RIGHT_3_TM=58.214
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=830
PRIMER_PAIR_4_PENALTY=3.843313
PRIMER_LEFT_4_PENALTY=1.057129
PRIMER_RIGHT_4_PENALTY=2.786184
PRIMER_LEFT_4_SEQUENCE=AAGAGCGTCCACCAAAGAGT
PRIMER_RIGHT_4_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_4=806,20
PRIMER_RIGHT_4=1404,21
PRIMER_LEFT_4_TM=58.943
PRIMER_RIGHT_4_TM=58.214
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=599
PRIMER_PAIR_5_PENALTY=4.070714
PRIMER_LEFT_5_PENALTY=1.284530
PRIMER_RIGHT_5_PENALTY=2.786184
PRIMER_LEFT_5_SEQUENCE=TGGTCTCCTGTTCCTCCTTT
PRIMER_RIGHT_5_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_5=780,20
PRIMER_RIGHT_5=1404,21
PRIMER_LEFT_5_TM=58.715
PRIMER_RIGHT_5_TM=58.214
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=625
PRIMER_PAIR_6_PENALTY=4.399327
PRIMER_LEFT_6_PENALTY=1.613143
PRIMER_RIGHT_6_PENALTY=2.786184
PRIMER_LEFT_6_SEQUENCE=GGTCTCCTGTTCCTCCTTTCA
PRIMER_RIGHT_6_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_6=781,21
PRIMER_RIGHT_6=1404,21
PRIMER_LEFT_6_TM=60.613
PRIMER_RIGHT_6_TM=58.214
PRIMER_LEFT_6_GC_PERCENT=52.381
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=624
PRIMER_PAIR_7_PENALTY=4.399327
PRIMER_LEFT_7_PENALTY=1.613143
PRIMER_RIGHT_7_PENALTY=2.786184
PRIMER_LEFT_7_SEQUENCE=TGGTCTCCTGTTCCTCCTTTC
PRIMER_RIGHT_7_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_7=780,21
PRIMER_RIGHT_7=1404,21
PRIMER_LEFT_7_TM=60.613
PRIMER_RIGHT_7_TM=58.214
PRIMER_LEFT_7_GC_PERCENT=52.381
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=625
PRIMER_PAIR_8_PENALTY=4.585338
PRIMER_LEFT_8_PENALTY=1.799154
PRIMER_RIGHT_8_PENALTY=2.786184
PRIMER_LEFT_8_SEQUENCE=CTTTGATGCAACCAGGAAAC
PRIMER_RIGHT_8_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_8=574,20
PRIMER_RIGHT_8=1404,21
PRIMER_LEFT_8_TM=58.201
PRIMER_RIGHT_8_TM=58.214
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=831
PRIMER_PAIR_9_PENALTY=4.681655
PRIMER_LEFT_9_PENALTY=1.895470
PRIMER_RIGHT_9_PENALTY=2.786184
PRIMER_LEFT_9_SEQUENCE=TAAGAGCGTCCACCAAAGAG
PRIMER_RIGHT_9_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_9=805,20
PRIMER_RIGHT_9=1404,21
PRIMER_LEFT_9_TM=58.105
PRIMER_RIGHT_9_TM=58.214
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=600
PRIMER_PAIR_10_PENALTY=4.695662
PRIMER_LEFT_10_PENALTY=1.909478
PRIMER_RIGHT_10_PENALTY=2.786184
PRIMER_LEFT_10_SEQUENCE=ACTTTGATGCAACCAGGAAAC
PRIMER_RIGHT_10_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_10=573,21
PRIMER_RIGHT_10=1404,21
PRIMER_LEFT_10_TM=59.091
PRIMER_RIGHT_10_TM=58.214
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=832
PRIMER_PAIR_11_PENALTY=4.730014
PRIMER_LEFT_11_PENALTY=1.943830
PRIMER_RIGHT_11_PENALTY=2.786184
PRIMER_LEFT_11_SEQUENCE=ATGGTCTCCTGTTCCTCCTTT
PRIMER_RIGHT_11_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_11=779,21
PRIMER_RIGHT_11=1404,21
PRIMER_LEFT_11_TM=59.056
PRIMER_RIGHT_11_TM=58.214
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=626
PRIMER_PAIR_12_PENALTY=4.792433
PRIMER_LEFT_12_PENALTY=2.006249
PRIMER_RIGHT_12_PENALTY=2.786184
PRIMER_LEFT_12_SEQUENCE=TAAGAGCGTCCACCAAAGAGT
PRIMER_RIGHT_12_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_12=805,21
PRIMER_RIGHT_12=1404,21
PRIMER_LEFT_12_TM=58.994
PRIMER_RIGHT_12_TM=58.214
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.1000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=600
PRIMER_PAIR_13_PENALTY=4.946997
PRIMER_LEFT_13_PENALTY=2.160813
PRIMER_RIGHT_13_PENALTY=2.786184
PRIMER_LEFT_13_SEQUENCE=GAGCGTCCACCAAAGAGTCC
PRIMER_RIGHT_13_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_13=808,20
PRIMER_RIGHT_13=1404,21
PRIMER_LEFT_13_TM=62.161
PRIMER_RIGHT_13_TM=58.214
PRIMER_LEFT_13_GC_PERCENT=60.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=597
PRIMER_PAIR_14_PENALTY=4.987331
PRIMER_LEFT_14_PENALTY=0.308722
PRIMER_RIGHT_14_PENALTY=4.678609
PRIMER_LEFT_14_SEQUENCE=CACTTTGATGCAACCAGGAA
PRIMER_RIGHT_14_SEQUENCE=GGCTCTTAAGTATGCGACAG
PRIMER_LEFT_14=572,20
PRIMER_RIGHT_14=1403,20
PRIMER_LEFT_14_TM=59.691
PRIMER_RIGHT_14_TM=55.321
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=832
PRIMER_PAIR_15_PENALTY=4.989181
PRIMER_LEFT_15_PENALTY=2.202997
PRIMER_RIGHT_15_PENALTY=2.786184
PRIMER_LEFT_15_SEQUENCE=TAGAACAACCCAAGTCTGCC
PRIMER_RIGHT_15_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_15=553,20
PRIMER_RIGHT_15=1404,21
PRIMER_LEFT_15_TM=57.797
PRIMER_RIGHT_15_TM=58.214
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=852
PRIMER_PAIR_16_PENALTY=5.043608
PRIMER_LEFT_16_PENALTY=2.257424
PRIMER_RIGHT_16_PENALTY=2.786184
PRIMER_LEFT_16_SEQUENCE=GGTCTCCTGTTCCTCCTTTC
PRIMER_RIGHT_16_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_16=781,20
PRIMER_RIGHT_16=1404,21
PRIMER_LEFT_16_TM=57.743
PRIMER_RIGHT_16_TM=58.214
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=624
PRIMER_PAIR_17_PENALTY=5.077139
PRIMER_LEFT_17_PENALTY=2.290955
PRIMER_RIGHT_17_PENALTY=2.786184
PRIMER_LEFT_17_SEQUENCE=GTAGAACAACCCAAGTCTGCC
PRIMER_RIGHT_17_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_17=552,21
PRIMER_RIGHT_17=1404,21
PRIMER_LEFT_17_TM=58.709
PRIMER_RIGHT_17_TM=58.214
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=853
PRIMER_PAIR_18_PENALTY=5.103952
PRIMER_LEFT_18_PENALTY=2.317768
PRIMER_RIGHT_18_PENALTY=2.786184
PRIMER_LEFT_18_SEQUENCE=AGTAGAACAACCCAAGTCTGCC
PRIMER_RIGHT_18_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_18=551,22
PRIMER_RIGHT_18=1404,21
PRIMER_LEFT_18_TM=59.682
PRIMER_RIGHT_18_TM=58.214
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=854
PRIMER_PAIR_19_PENALTY=5.233374
PRIMER_LEFT_19_PENALTY=0.554765
PRIMER_RIGHT_19_PENALTY=4.678609
PRIMER_LEFT_19_SEQUENCE=GAACAACCCAAGTCTGCCAC
PRIMER_RIGHT_19_SEQUENCE=GGCTCTTAAGTATGCGACAG
PRIMER_LEFT_19=555,20
PRIMER_RIGHT_19=1403,20
PRIMER_LEFT_19_TM=60.555
PRIMER_RIGHT_19_TM=55.321
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=849
PRIMER_PAIR_20_PENALTY=5.274400
PRIMER_LEFT_20_PENALTY=2.488216
PRIMER_RIGHT_20_PENALTY=2.786184
PRIMER_LEFT_20_SEQUENCE=TTTGTCCAGTAGAACAACCCAA
PRIMER_RIGHT_20_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_20=544,22
PRIMER_RIGHT_20=1404,21
PRIMER_LEFT_20_TM=59.512
PRIMER_RIGHT_20_TM=58.214
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=10.0000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=861
PRIMER_PAIR_21_PENALTY=5.274400
PRIMER_LEFT_21_PENALTY=2.488216
PRIMER_RIGHT_21_PENALTY=2.786184
PRIMER_LEFT_21_SEQUENCE=TTTTGTCCAGTAGAACAACCCA
PRIMER_RIGHT_21_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_21=543,22
PRIMER_RIGHT_21=1404,21
PRIMER_LEFT_21_TM=59.512
PRIMER_RIGHT_21_TM=58.214
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=862
PRIMER_PAIR_22_PENALTY=5.281413
PRIMER_LEFT_22_PENALTY=2.495229
PRIMER_RIGHT_22_PENALTY=2.786184
PRIMER_LEFT_22_SEQUENCE=AGAACAACCCAAGTCTGCCAC
PRIMER_RIGHT_22_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_22=554,21
PRIMER_RIGHT_22=1404,21
PRIMER_LEFT_22_TM=61.495
PRIMER_RIGHT_22_TM=58.214
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=851
PRIMER_PAIR_23_PENALTY=5.390477
PRIMER_LEFT_23_PENALTY=0.711868
PRIMER_RIGHT_23_PENALTY=4.678609
PRIMER_LEFT_23_SEQUENCE=CCAAGTCTGCCACTTTGATG
PRIMER_RIGHT_23_SEQUENCE=GGCTCTTAAGTATGCGACAG
PRIMER_LEFT_23=562,20
PRIMER_RIGHT_23=1403,20
PRIMER_LEFT_23_TM=59.288
PRIMER_RIGHT_23_TM=55.321
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=842
PRIMER_PAIR_24_PENALTY=5.551234
PRIMER_LEFT_24_PENALTY=2.765049
PRIMER_RIGHT_24_PENALTY=2.786184
PRIMER_LEFT_24_SEQUENCE=CTTTGATGCAACCAGGAAACC
PRIMER_RIGHT_24_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_24=574,21
PRIMER_RIGHT_24=1404,21
PRIMER_LEFT_24_TM=61.765
PRIMER_RIGHT_24_TM=58.214
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=831
PRIMER_PAIR_25_PENALTY=5.552179
PRIMER_LEFT_25_PENALTY=0.873570
PRIMER_RIGHT_25_PENALTY=4.678609
PRIMER_LEFT_25_SEQUENCE=TTTGATGCAACCAGGAAACC
PRIMER_RIGHT_25_SEQUENCE=GGCTCTTAAGTATGCGACAG
PRIMER_LEFT_25=575,20
PRIMER_RIGHT_25=1403,20
PRIMER_LEFT_25_TM=60.874
PRIMER_RIGHT_25_TM=55.321
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=829
PRIMER_PAIR_26_PENALTY=5.631517
PRIMER_LEFT_26_PENALTY=2.845332
PRIMER_RIGHT_26_PENALTY=2.786184
PRIMER_LEFT_26_SEQUENCE=ATGGTCTCCTGTTCCTCCTTTC
PRIMER_RIGHT_26_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_26=779,22
PRIMER_RIGHT_26=1404,21
PRIMER_LEFT_26_TM=60.845
PRIMER_RIGHT_26_TM=58.214
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=626
PRIMER_PAIR_27_PENALTY=5.644231
PRIMER_LEFT_27_PENALTY=2.858047
PRIMER_RIGHT_27_PENALTY=2.786184
PRIMER_LEFT_27_SEQUENCE=TTGTCCAGTAGAACAACCCAAG
PRIMER_RIGHT_27_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_27=545,22
PRIMER_RIGHT_27=1404,21
PRIMER_LEFT_27_TM=59.142
PRIMER_RIGHT_27_TM=58.214
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=860
PRIMER_PAIR_28_PENALTY=5.667653
PRIMER_LEFT_28_PENALTY=2.881468
PRIMER_RIGHT_28_PENALTY=2.786184
PRIMER_LEFT_28_SEQUENCE=TTGTCCAGTAGAACAACCCAA
PRIMER_RIGHT_28_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_28=545,21
PRIMER_RIGHT_28=1404,21
PRIMER_LEFT_28_TM=58.119
PRIMER_RIGHT_28_TM=58.214
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=10.0000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=860
PRIMER_PAIR_29_PENALTY=5.667653
PRIMER_LEFT_29_PENALTY=2.881468
PRIMER_RIGHT_29_PENALTY=2.786184
PRIMER_LEFT_29_SEQUENCE=TTTGTCCAGTAGAACAACCCA
PRIMER_RIGHT_29_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_29=544,21
PRIMER_RIGHT_29=1404,21
PRIMER_LEFT_29_TM=58.119
PRIMER_RIGHT_29_TM=58.214
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=861
PRIMER_PAIR_30_PENALTY=5.685325
PRIMER_LEFT_30_PENALTY=2.899141
PRIMER_RIGHT_30_PENALTY=2.786184
PRIMER_LEFT_30_SEQUENCE=TATGGTCTCCTGTTCCTCCTTT
PRIMER_RIGHT_30_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_30=778,22
PRIMER_RIGHT_30=1404,21
PRIMER_LEFT_30_TM=59.101
PRIMER_RIGHT_30_TM=58.214
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=627
PRIMER_PAIR_31_PENALTY=5.735738
PRIMER_LEFT_31_PENALTY=1.057129
PRIMER_RIGHT_31_PENALTY=4.678609
PRIMER_LEFT_31_SEQUENCE=AAGAGCGTCCACCAAAGAGT
PRIMER_RIGHT_31_SEQUENCE=GGCTCTTAAGTATGCGACAG
PRIMER_LEFT_31=806,20
PRIMER_RIGHT_31=1403,20
PRIMER_LEFT_31_TM=58.943
PRIMER_RIGHT_31_TM=55.321
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.1000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=598
PRIMER_PAIR_32_PENALTY=5.795009
PRIMER_LEFT_32_PENALTY=3.008825
PRIMER_RIGHT_32_PENALTY=2.786184
PRIMER_LEFT_32_SEQUENCE=CTATGGTCTCCTGTTCCTCCTTT
PRIMER_RIGHT_32_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_32=777,23
PRIMER_RIGHT_32=1404,21
PRIMER_LEFT_32_TM=60.009
PRIMER_RIGHT_32_TM=58.214
PRIMER_LEFT_32_GC_PERCENT=47.826
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=628
PRIMER_PAIR_33_PENALTY=5.835685
PRIMER_LEFT_33_PENALTY=3.049500
PRIMER_RIGHT_33_PENALTY=2.786184
PRIMER_LEFT_33_SEQUENCE=TTGTCCAGTAGAACAACCCAAGT
PRIMER_RIGHT_33_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_33=545,23
PRIMER_RIGHT_33=1404,21
PRIMER_LEFT_33_TM=59.950
PRIMER_RIGHT_33_TM=58.214
PRIMER_LEFT_33_GC_PERCENT=43.478
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=860
PRIMER_PAIR_34_PENALTY=5.948534
PRIMER_LEFT_34_PENALTY=3.162349
PRIMER_RIGHT_34_PENALTY=2.786184
PRIMER_LEFT_34_SEQUENCE=GTCTCCTGTTCCTCCTTTCA
PRIMER_RIGHT_34_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_34=782,20
PRIMER_RIGHT_34=1404,21
PRIMER_LEFT_34_TM=56.838
PRIMER_RIGHT_34_TM=58.214
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.3000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=623
PRIMER_PAIR_35_PENALTY=5.963138
PRIMER_LEFT_35_PENALTY=1.284530
PRIMER_RIGHT_35_PENALTY=4.678609
PRIMER_LEFT_35_SEQUENCE=TGGTCTCCTGTTCCTCCTTT
PRIMER_RIGHT_35_SEQUENCE=GGCTCTTAAGTATGCGACAG
PRIMER_LEFT_35=780,20
PRIMER_RIGHT_35=1403,20
PRIMER_LEFT_35_TM=58.715
PRIMER_RIGHT_35_TM=55.321
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=624
PRIMER_PAIR_36_PENALTY=6.019361
PRIMER_LEFT_36_PENALTY=3.233177
PRIMER_RIGHT_36_PENALTY=2.786184
PRIMER_LEFT_36_SEQUENCE=CCAGTAGAACAACCCAAGTCTG
PRIMER_RIGHT_36_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_36=549,22
PRIMER_RIGHT_36=1404,21
PRIMER_LEFT_36_TM=58.767
PRIMER_RIGHT_36_TM=58.214
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=856
PRIMER_PAIR_37_PENALTY=6.068795
PRIMER_LEFT_37_PENALTY=3.282611
PRIMER_RIGHT_37_PENALTY=2.786184
PRIMER_LEFT_37_SEQUENCE=TGTCCAGTAGAACAACCCAAG
PRIMER_RIGHT_37_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_37=546,21
PRIMER_RIGHT_37=1404,21
PRIMER_LEFT_37_TM=57.717
PRIMER_RIGHT_37_TM=58.214
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=859
PRIMER_PAIR_38_PENALTY=6.087412
PRIMER_LEFT_38_PENALTY=3.301227
PRIMER_RIGHT_38_PENALTY=2.786184
PRIMER_LEFT_38_SEQUENCE=GTTTTGTCCAGTAGAACAACCCA
PRIMER_RIGHT_38_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_38=542,23
PRIMER_RIGHT_38=1404,21
PRIMER_LEFT_38_TM=60.301
PRIMER_RIGHT_38_TM=58.214
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=863
PRIMER_PAIR_39_PENALTY=6.120014
PRIMER_LEFT_39_PENALTY=3.333829
PRIMER_RIGHT_39_PENALTY=2.786184
PRIMER_LEFT_39_SEQUENCE=CACCGTGTTTTGTCCAGTAG
PRIMER_RIGHT_39_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_39=536,20
PRIMER_RIGHT_39=1404,21
PRIMER_LEFT_39_TM=56.666
PRIMER_RIGHT_39_TM=58.214
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.4000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=869
PRIMER_PAIR_40_PENALTY=6.160848
PRIMER_LEFT_40_PENALTY=3.374664
PRIMER_RIGHT_40_PENALTY=2.786184
PRIMER_LEFT_40_SEQUENCE=TGTCCAGTAGAACAACCCAAGT
PRIMER_RIGHT_40_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_40=546,22
PRIMER_RIGHT_40=1404,21
PRIMER_LEFT_40_TM=58.625
PRIMER_RIGHT_40_TM=58.214
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=859
PRIMER_PAIR_41_PENALTY=6.186834
PRIMER_LEFT_41_PENALTY=3.400649
PRIMER_RIGHT_41_PENALTY=2.786184
PRIMER_LEFT_41_SEQUENCE=AACTATGGTCTCCTGTTCCTCC
PRIMER_RIGHT_41_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_41=775,22
PRIMER_RIGHT_41=1404,21
PRIMER_LEFT_41_TM=58.599
PRIMER_RIGHT_41_TM=58.214
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=630
PRIMER_PAIR_42_PENALTY=6.208399
PRIMER_LEFT_42_PENALTY=3.422215
PRIMER_RIGHT_42_PENALTY=2.786184
PRIMER_LEFT_42_SEQUENCE=TTTGTCCAGTAGAACAACCCAAG
PRIMER_RIGHT_42_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_42=544,23
PRIMER_RIGHT_42=1404,21
PRIMER_LEFT_42_TM=60.422
PRIMER_RIGHT_42_TM=58.214
PRIMER_LEFT_42_GC_PERCENT=43.478
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=861
PRIMER_PAIR_43_PENALTY=6.213051
PRIMER_LEFT_43_PENALTY=3.426867
PRIMER_RIGHT_43_PENALTY=2.786184
PRIMER_LEFT_43_SEQUENCE=CGTTCTCTATGACCAAAGGTTGT
PRIMER_RIGHT_43_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_43=619,23
PRIMER_RIGHT_43=1404,21
PRIMER_LEFT_43_TM=59.573
PRIMER_RIGHT_43_TM=58.214
PRIMER_LEFT_43_GC_PERCENT=43.478
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=786
PRIMER_PAIR_44_PENALTY=6.226008
PRIMER_LEFT_44_PENALTY=3.439823
PRIMER_RIGHT_44_PENALTY=2.786184
PRIMER_LEFT_44_SEQUENCE=TGTCCAGTAGAACAACCCAA
PRIMER_RIGHT_44_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_44=546,20
PRIMER_RIGHT_44=1404,21
PRIMER_LEFT_44_TM=56.560
PRIMER_RIGHT_44_TM=58.214
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=10.0000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=859
PRIMER_PAIR_45_PENALTY=6.226008
PRIMER_LEFT_45_PENALTY=3.439823
PRIMER_RIGHT_45_PENALTY=2.786184
PRIMER_LEFT_45_SEQUENCE=TTGTCCAGTAGAACAACCCA
PRIMER_RIGHT_45_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_45=545,20
PRIMER_RIGHT_45=1404,21
PRIMER_LEFT_45_TM=56.560
PRIMER_RIGHT_45_TM=58.214
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=860
PRIMER_PAIR_46_PENALTY=6.227915
PRIMER_LEFT_46_PENALTY=3.441731
PRIMER_RIGHT_46_PENALTY=2.786184
PRIMER_LEFT_46_SEQUENCE=TGTCCAGTAGAACAACCCAAGTC
PRIMER_RIGHT_46_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_46=546,23
PRIMER_RIGHT_46=1404,21
PRIMER_LEFT_46_TM=60.442
PRIMER_RIGHT_46_TM=58.214
PRIMER_LEFT_46_GC_PERCENT=47.826
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=859
PRIMER_PAIR_47_PENALTY=6.236126
PRIMER_LEFT_47_PENALTY=3.449941
PRIMER_RIGHT_47_PENALTY=2.786184
PRIMER_LEFT_47_SEQUENCE=TAGAACAACCCAAGTCTGCCAC
PRIMER_RIGHT_47_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_47=553,22
PRIMER_RIGHT_47=1404,21
PRIMER_LEFT_47_TM=61.450
PRIMER_RIGHT_47_TM=58.214
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=852
PRIMER_PAIR_48_PENALTY=6.244308
PRIMER_LEFT_48_PENALTY=3.458124
PRIMER_RIGHT_48_PENALTY=2.786184
PRIMER_LEFT_48_SEQUENCE=AACTATGGTCTCCTGTTCCTCCT
PRIMER_RIGHT_48_SEQUENCE=TGGCTCTTAAGTATGCGACAG
PRIMER_LEFT_48=775,23
PRIMER_RIGHT_48=1404,21
PRIMER_LEFT_48_TM=59.542
PRIMER_RIGHT_48_TM=58.214
PRIMER_LEFT_48_GC_PERCENT=47.826
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=630
PRIMER_PAIR_49_PENALTY=6.291752
PRIMER_LEFT_49_PENALTY=1.613143
PRIMER_RIGHT_49_PENALTY=4.678609
PRIMER_LEFT_49_SEQUENCE=GGTCTCCTGTTCCTCCTTTCA
PRIMER_RIGHT_49_SEQUENCE=GGCTCTTAAGTATGCGACAG
PRIMER_LEFT_49=781,21
PRIMER_RIGHT_49=1403,20
PRIMER_LEFT_49_TM=60.613
PRIMER_RIGHT_49_TM=55.321
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=623
=
SEQUENCE_ID=MAGIC.175_4_1771938_1772648_edi-0_hi-0_17552_13904_TRUE
SEQUENCE_TARGET=950,810
SEQUENCE_TEMPLATE=GGATCGAAAACCCAAATGATAACTCCACANNGCAGAGAAAATNCATCGGTTGATAACTGGAGATAATNCCGGTGTGAGAGACCACNGTATACAAACCTTATACAAGGACAATCAAAATTCAAANGAATCGATATAACACACGANCAGAAACATCGACAAATGGATGCATTTATCCGTGAAATCAAGTTGAATTGATAGGCTTTCCAGATTGAAGAATGAATCANAATCGATAGTAATGATGTACCACCGTCANGATGTGGCAAAGAACATAAATTTGAGATNTTCGGTTTGNCGTTATTTACCACAAGAAACTGAAAACTTTGCAGAAATTAGAGAAAAGNAATAACAAACACGAGAATAGCCGACGCNAGAGAGACCGACGCCGGCCAAAGTGATGAAGTTTTGTGAAGTTGCCTTCAATGGCAGATCGCTTGGNAGAGAGAACGGTTGTCGAGTCGTTTGNGTAACAAAGTNATATTGAAGGGAAGTTAAAANGAGTAATTTGGCTAACAAAATCNNAANTTTTATTAGATTTTGGACCTTAANTTTTAAATAATATATTCAAATTTGTTGTGTGTTTTCAACTTTTTATNTTATATATTNNNATNTNNTATAATATTATANNNNNAATAAANNAATAAAGNNNAAAAGTGACGAGANANTAATNAANTNNNNTTCTTATTNTGTTNTGATACTTACAATNNNNNNNNGAGAACCAANGTATATATACAAGAGTTGCTTGAAGAGAAAGTTAAATTTAGATAATAGGATAAGTTTTAGTTAATTGGAAAATAAGATACTTANGAAGTAAGTNANAAAAANAANAATAGTAGTATTTATCTAATCTCAANAGTATCTAAATANTTTNTGTNNNNNGTAAAGTTNTATNANACAANTTNCGAGAANATCANAAAACNATANTCATACATTTCACNCNNNGATTCATCAATNAGACCAAACATANNTTTNAAAAATCAGTNTTTACACACAATCAATAAANNTGATNNNNATACATATATATAACTCAAAAAGGATCAANNATTGATTTACATACACACNTTNTANATTTGTATNTATAGANGATCTACAAAAANAATCTGTTNNNTCGCCATTGTTGAAATNAAAACATCNTAGTACNAATCTCNTNGTNTGTGTTGTGTGACACTGNTTCNGTCTTCATGTTATGTTCACTGCCAAAAACCAAAAACATGAAGAATGTTNTCGGTTACAAGAGGAAAGTGATGAAAAATGAAAATATGGATTNANAGTAGAAANANATAAAATTACTGATTGTCTTTTTGAGCAGCCATAGAAGAAGAGCAAGTTCGATGCTGAAGATCATACGCAACCATGTTTTATCCACCAAGAATCCGTAAACAGTGATCCCTGCTCTGTTGTTCTCTAGATAGGTCACTNNATATATACACAATAGAGAAACCAAATCAGTTAAAAAGGTGAAAATGATTTATTCNAATCATNGTCTAATTCAAGATTTTATNTACCTAGAGCTTGACGTTTCTGAGAAGAAATGGCGCGGGCAAAGATTGGATGTATCTCAAGATCANTGTCATCTCCTTCTCCTTCTTCATCATCATCAGACGATTGAACAACATTACGACGACGCGATAAAATCTGGGAATGTTGTGTAGTTGTTGGACATTTAGGAGTCTCTCCGTCATAAAGAACATCAAATGTATCTAAAGACGCGCANACGTTCCACTTTGTGGCGATGCTCGTTACTGANTGAGCTTTGTGAGTCATTNGTGTTGCACTTTTCAAGCATATGAATAGTCCTGACACCAAGCTTNTGGAGCATAACTGTAAGAAACAGAGACATTGATCAATATAATGTTTCAAAAACTTGCATCCCTATATGAAAGTGAAGAGGAANTCTTACNGCGAGCTCGCCAACTTCGTAGATNTTAAANGGAACACTAGCTCTGATGGTAGTNAGCAATGCCATGAACTGAGTGGCNGTNACGAAAAACAANGACAAAANNNNGAATCNNCTGAANCGGTGACTGACAATCTTCAGTTCACGACGGATTTTGAGATGCTCNGCGAGTATGGAACTGAAATCTTTGATCTCAGAGGCGAAGCAGCGTGCGAATTCATCAAGACGGAGGACCTGNAGGTGGCAGATGTTTTGGTAGAGGATACAAGCAATGATGAAGAGTGATGTACGGTAGAGCCAAGAAGAGAGCTGGAGTGTGCAGGCTAAGNCATGGCTCAGCGTTGGGTTTATAATGTANGGGATTTGGTTNAAGCCTGAAGCATACCACCAAATGCGATAGATAGCTTGAAGCGTTGTCGATGGAAGGACAAAGATAGCNAGTAGCTTCATTGATCTCTGCAACAATAAAACAGAGGAGACTTTCAAAAACATCAGGATTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTNNAGTTCTNNGCCTGCGAGNTCAGTTAACCNAACCNAACNGAGTCGGTTATCTCAGTATTTCGGTTNATGNAATTCTCCGANTTGAACCNATTTTTTGGGTTTTNCCN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4218972_4219011_mt-0_ct-1_2567987_15086_TRUE
SEQUENCE_TARGET=950,139
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAACGTTTTAAAATTATGTATTGNAAANTTTATTTTTANTTTTNAAANANNAAAAAANAGTTTACGATAGAATTTGTTNAAAATCNCAAAAAAGTGGGTTTTGGATTTTNAGAACAAATGANTANAATCTATAAAAATCTAGATTCCNTATAATTTAGGNAATTAATNTTTTATNAAATCATNAATGNGTGAAAAANNGGATTTTNCAAAATCAAAAGCCANAANNNNCTTNAAAAACCAGTACNAGTCAAGGCNNNNNNNNTTTTNTTTTNTNAATCTGTTTATGTTTANGTNATACAATTAATAATAATANNAATAAATATAGTAATATNGATTTNAAAATGAAGNANAANTGTTATTATNNTATATNTTACAAAANNNNNNNNAAANATTTTTAATAAAATAGANAATGTGACAAATTAAAGAGAAATTAAANNNNAAAAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5913336_5913878_no-0_oy-0_38141_17990_TRUE
SEQUENCE_TARGET=950,642
SEQUENCE_TEMPLATE=CTNGTAACAACTCTTAGCACAAAGACAATCGTCTTCGGTGCAACNAATCCTAAAGGACAATATGATCCCGATCAGTGTATCCACTTCAGATTTAGTAGACNTTGCTTACATACATNCATGCTNTTTTTCAAATNNAAGTTGGTGATGTCTCTNAAAAAANATNNTTCTTCCCTCAAGCATCAGTNGGNCAAGCATTCCTGNTTTCCCTTTGNATTTNTTTNGATATTTCTCNTTAGAANNTCCTTNACTGNATCTAGCTCTTTCTGTCAATACTGCACTTTCNGGTCCCTTGTTGAGCAGGTTTGACATTGTACTTGTTCTCTTAGATACAAAAAATCCNGAATGGGATGCAGTTGTATCNTCTCACATNCTTGCCGAGGTANGTTTCTTTAAAGAATNTATNAATATTGTGTTGTATACTGATANTGAGNCTTCTTAAGNAGAGTATTTGTGACAAGATATATAACTNATNTGANTTTNNAANNATTGATGAAATGAACCNAGGTGCAAATAGAGCAAGNCAGAGAGGTCGATGATCTGACNACTATTTGGCCANTACCGATGCTTCAAAGGTATCATATNCGTACANAGATCATGAGATCCATAANCCANGAGAGTNNCAATTATTCCTTGCACNTAAATCNGNNANGACAAATGTNCTTGANCTTAGCTGATANTACATTCNTGGNTGCTANTAACTTNTTANATCCTGACCTTNGCAGATACNTTCAGTTTGTGAAAAAAAACTTCANACCTGTCCTATCAAAGGAAGCAGAAGAAATNATTTCAAGTTATTATCGNCTCCAAAGAAGATCTTCAACTCATAATGCAGGTATCTCNTNAGAAATACTTCGTNGTTCTNNAGATGCTAGTTTGGTATTAACATGGGGTGAGGGNAGCTGANTATAGTGGACCANTGTCCAGATTTGAATNNNNNTATTTNATGGCTGAGANNTNTTANTCNTTANNTNTATNNNAANTTNNNNCGANANCNCTTCNTNTTNNNGGNACNNGGTCTAGAAAATCNNTAGNTCNCATGNGAACTNTNANTAAGCATANCCTTNCTNAAANGGATTTTAGGACTNGNTTGGTGGGTANNNNNNNNNCAACTAAGATNGATCCNACAATGGTTNATATCTTNCATNTAATTTGATTNTNCTCTGAACTGNAGCAAGAACAACNGTGCGNATGTTGGAAAGCTTAATCCGTTTGGCTCAAGGTACCTCTNCTGTTATCNTCTTTATCNATCAAAATACTCCAANAACAGNNNCAANNNNNNGGATATTAANNGTNTTATGGTGTCACNCAGCTCATGCAAGGCTTATGTTCAGAAATGAGGTCACGCGATTAGATGCCATAACAGCNATCNTNTGCATTGAATCATCGATGACNATTTCAGCCATAGTAGACAGTATGGGGAATGCGCTACACTCAAATTTNTCNGAAGAACCAGATCAGGAATGTATCCTTTTTCGGCATTANNTAACTNTTGTTCCTANATATGTCCAAAGTCCGNCTTTNGNTACTNNNGANTCATCTTCTNGAGCATTGCGGCTTGNANNTTTTGTATNNTTTNCCCTAACNTAAAGAAACAAACAGACGNGAAGCAAGNNAGATCATCCTNGGAAANCTAAGANNNGANGACATCTATTAGNNNNTGGNTGCGATGAGTAGTGANCCNACAGNAAAGAGATAAGTTTGCAANAATCTCCNTCACGTTTCTCATNCAAGTTTTCNTTTGTATAAAGTGCATNATGTGTGATTNTGCAGTNGANATGGCTCNNNNNGTTCCTTTAGAGTATATCTAGAGAGCAAACTTANCTTNANAAGANNTATGTATTCCANCTCCNTGTCCNNTTCAATCAAATAGGTAAAGNNANNACAGANTTTNTTATCTTNCGACNGNTGATATCAACCGCTTTCCATAACATTCCACTTGCCATTGCAANACATNGTTNAACCNAGAANGCATTTTGAAAACGTATGTNNAAGTANTTCTTNAGAACTNTTAGTTGACNATGGAATTTTTGCAAGGTAGCTATGTACCCTGTGGATCATNTCTCGGTCCAANTCACTTATTAGNAANACAGTGATGATATTGATNGNTATGTGTANGTGACAATTGCTTAATTTATAATNGAATNAAAATTGCTCGTCCCACANTCNAAACAATGAACTTTATTTCCTAGTCAACNAANCTACGATGACGAGTGTCATATTANGGCGATGGTTTNAGACACTTAGATACTTNGCCATATAGTGTGNATACTTGTNGAACACCNGTTCNATAATTGCATCTCCNAAATGAGCAACGAGCAATGGTTNGGNGATGCATCTTGCCCANCTTGCATGCTTTATACCAGCTTCNANCTGAAGTGATTGCTCCTCATCTTCTTCNTCACNGTCAATNTTNTATGGGACAACATGNTCNAATTTCTNGATCTTGGTGANCTCGAANGAACCTTNNNTTAAGATAANTTCTNTTACCTCTTCTTCGTTTGGANTGTAGAANGNNATGTTAAATGATTNNACCATTNAANNG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_2_9253515_9253623_tsu-0_can-0_687550_1340_TRUE
SEQUENCE_TARGET=950,208
SEQUENCE_TEMPLATE=TATATTGTGGANACTNTATGGCTTATNTCATCTTATGTTCTTNGTAATCCATTCTAAGTAGTGATTNGAAGNNNCCANGTTTGAGAATTGGTNAGTGGTTTTGTACATGGCTAGACTGATCCNGACTATGGTCNNTAACTATTCCTTGGAAATNGCTATGTTAAGTATAGGAGTTGAGCAATGGNTTTGACCAAGAAGCAGCTGCTGTAGTAATGGCACGACTTATTTCNTNCAAAATGGAGACTCAGGTACTNGATGATGAGAGCTNGTTAGATTTNTNAAGCTGAATATCNGNTAGATGNTNTCNTGTTTTAGTGTTTCTTAGGTTTCTTATNTCTTCTTTTACAGCNTGAGTTTAATCCCCAAAGGTGGGTCGATAAAGCNTTGATAAATCTATGCACCTGGNTTGGAGATTACCAGAAAGGCAATCCNTCTTCCTTTAGCTTATCTTCTCAGTTATCAATTTTCCCGCAGTTTGTNTTTCACTTGCGCCGATCTCAGTTTGTCCAGGTACTTATCCACACTGTCCCTANNTGCANGATCGACTGAAATCTTTTTAACGCACAATTTTATCCTTTTGGGCTCATTTGGCTTTNCCTTNTGTAGGTCTTTAACAACAGCCCAGACGAGACAGCATATTTTCGAATGATATTGTANAGAGAGAACGTTTCTAATTCAGTCGTGATGATTCAACCTTCACTGANNTCTTTCTCATTCCATTCACCGCCAGAGCCAATTCTTCTTGATGTAGCATCCATTGCAGCTGACAGAATCNTACTGTTGGATTCATACTTCACCCTTGTAATATTTCACGGCTCTACAATCGCTCAATGGCGAAAAGCTGGATACCATAATCAACCTGAACACCAGGTAACTATNATCTATCATCTTAANAGATAATATGATANAAAGTNGTTGCCTTNTAGAGTCTGATCTTCATACAACTTGTCTTNTTTGTGCCAGGCATTTGGGCATCTGTTGCAGTCACCTCGTGACTACGNAGACACAATCATGAGTGAAAGGTTTCCCACTCCGCGTCTTGTTATATGCGATCAGTATGGTTCACAGGTACCTCNCCTTNTTTGCTCATGCCTCCATNNNNNNNNNTNANTNNTNCATTNNTTGNNNNTNATNGNTNNTTNTNGTTNTTTNTNNTNCTNTNTNTTNNTNTNTNTTNTNGCANNNNNGNNTNCTTNTNNNNNANNTGNNTCNTTNTNNNGGNNANNNTNANTTTTNCGGNNNANGTNANNTTNTCNNGNATNNNNTNANCNTNTNNNTANTNNTTGNTCATNTNANANGNNNCNNTGNNCATTNANNNNNGCNNNCNATTTNGNTANNNNNTTTGNNNNTTGTTNTNNNNGNGANANAGTNAGAAAAAGATTTAGGCAAGATTCGTNTTTTACATTGATCATACGAGATCAAAAAGGAAAANCACCAAAACCTTGATGAAGAAGTAAAACAGATGANGTTAGCTAAACCGAGAAAGTAAAACTATCTTAAACCTTGATGAAGAAGGAAAAACACCAAAACCTTAATNTTAANNNNNAAAAANAAAATCACCANAATAACTTNNATNTAAAAANNNNNAAAAGCAACAAACCCCATACTCNATATCATTCAGNCACAAAATATTTGTATAAGCGAAAATTGAAGATNGATNAAAGTTAAAGGNNNNNNNNNNNNNNNNGAAGAATGTACATACAATAANANAANGGTTACCTTTTACANTATNAAAAAGGTNAAGGGGTCAAATNTGAAGNAACAGTTAAATTAAGGTCCAANTATTNTTANTTNAAACGCTGCGTTTAGTAACTTGCTCTCCAAGGAGATCTAGCTTGTTCTACTCGCACGTGATTATAACCCNTNCATAACTGTNTATANANATAGACGTCGTCCTTGTGCTTGCAGAGAAAGATAAAAACAGAAAAAGANAAGGAACTTGCAGAGAAAGCAAAAAAGAAAGAAGAAGCGAAGAAGAGAAACTAAGATTCAGGTAACTGCAAGCTTTGAATCTCTTCNTTAATTTCTTGCTTATGAATTNCTTATGATCGATAATGAGNCTGGNGAGTTCTGTTTCACTGAATCCGCAGCAAATTTTGAGNAATTCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.891173
PRIMER_LEFT_0_PENALTY=0.306669
PRIMER_RIGHT_0_PENALTY=2.584504
PRIMER_LEFT_0_SEQUENCE=AACAACAGCCCAGACGAGAC
PRIMER_RIGHT_0_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_0=612,20
PRIMER_RIGHT_0=1608,22
PRIMER_LEFT_0_TM=60.307
PRIMER_RIGHT_0_TM=59.415
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.1000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=8.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=997
PRIMER_PAIR_1_PENALTY=3.144489
PRIMER_LEFT_1_PENALTY=0.559985
PRIMER_RIGHT_1_PENALTY=2.584504
PRIMER_LEFT_1_SEQUENCE=TAACAACAGCCCAGACGAGA
PRIMER_RIGHT_1_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_1=611,20
PRIMER_RIGHT_1=1608,22
PRIMER_LEFT_1_TM=59.440
PRIMER_RIGHT_1_TM=59.415
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=998
PRIMER_PAIR_2_PENALTY=3.195851
PRIMER_LEFT_2_PENALTY=0.306669
PRIMER_RIGHT_2_PENALTY=2.889182
PRIMER_LEFT_2_SEQUENCE=AACAACAGCCCAGACGAGAC
PRIMER_RIGHT_2_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_2=612,20
PRIMER_RIGHT_2=1608,21
PRIMER_LEFT_2_TM=60.307
PRIMER_RIGHT_2_TM=58.111
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=997
PRIMER_PAIR_3_PENALTY=3.267189
PRIMER_LEFT_3_PENALTY=0.682685
PRIMER_RIGHT_3_PENALTY=2.584504
PRIMER_LEFT_3_SEQUENCE=TTTAACAACAGCCCAGACGA
PRIMER_RIGHT_3_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_3=609,20
PRIMER_RIGHT_3=1608,22
PRIMER_LEFT_3_TM=59.317
PRIMER_RIGHT_3_TM=59.415
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=1000
PRIMER_PAIR_4_PENALTY=3.518930
PRIMER_LEFT_4_PENALTY=0.934426
PRIMER_RIGHT_4_PENALTY=2.584504
PRIMER_LEFT_4_SEQUENCE=ACGGCTCTACAATCGCTCAA
PRIMER_RIGHT_4_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_4=811,20
PRIMER_RIGHT_4=1608,22
PRIMER_LEFT_4_TM=60.934
PRIMER_RIGHT_4_TM=59.415
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=798
PRIMER_PAIR_5_PENALTY=3.571867
PRIMER_LEFT_5_PENALTY=0.682685
PRIMER_RIGHT_5_PENALTY=2.889182
PRIMER_LEFT_5_SEQUENCE=TTTAACAACAGCCCAGACGA
PRIMER_RIGHT_5_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_5=609,20
PRIMER_RIGHT_5=1608,21
PRIMER_LEFT_5_TM=59.317
PRIMER_RIGHT_5_TM=58.111
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=8.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=1000
PRIMER_PAIR_6_PENALTY=3.661453
PRIMER_LEFT_6_PENALTY=1.076949
PRIMER_RIGHT_6_PENALTY=2.584504
PRIMER_LEFT_6_SEQUENCE=TTAACAACAGCCCAGACGAG
PRIMER_RIGHT_6_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_6=610,20
PRIMER_RIGHT_6=1608,22
PRIMER_LEFT_6_TM=58.923
PRIMER_RIGHT_6_TM=59.415
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=8.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=999
PRIMER_PAIR_7_PENALTY=3.676004
PRIMER_LEFT_7_PENALTY=0.306669
PRIMER_RIGHT_7_PENALTY=3.369335
PRIMER_LEFT_7_SEQUENCE=AACAACAGCCCAGACGAGAC
PRIMER_RIGHT_7_SEQUENCE=GTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_7=612,20
PRIMER_RIGHT_7=1606,20
PRIMER_LEFT_7_TM=60.307
PRIMER_RIGHT_7_TM=56.631
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.1000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=8.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=995
PRIMER_PAIR_8_PENALTY=3.823608
PRIMER_LEFT_8_PENALTY=0.934426
PRIMER_RIGHT_8_PENALTY=2.889182
PRIMER_LEFT_8_SEQUENCE=ACGGCTCTACAATCGCTCAA
PRIMER_RIGHT_8_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_8=811,20
PRIMER_RIGHT_8=1608,21
PRIMER_LEFT_8_TM=60.934
PRIMER_RIGHT_8_TM=58.111
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=798
PRIMER_PAIR_9_PENALTY=3.874787
PRIMER_LEFT_9_PENALTY=1.290283
PRIMER_RIGHT_9_PENALTY=2.584504
PRIMER_LEFT_9_SEQUENCE=TTTAACAACAGCCCAGACGAG
PRIMER_RIGHT_9_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_9=609,21
PRIMER_RIGHT_9=1608,22
PRIMER_LEFT_9_TM=60.290
PRIMER_RIGHT_9_TM=59.415
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=8.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=1000
PRIMER_PAIR_10_PENALTY=3.890223
PRIMER_LEFT_10_PENALTY=1.305719
PRIMER_RIGHT_10_PENALTY=2.584504
PRIMER_LEFT_10_SEQUENCE=TAACAACAGCCCAGACGAGAC
PRIMER_RIGHT_10_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_10=611,21
PRIMER_RIGHT_10=1608,22
PRIMER_LEFT_10_TM=60.306
PRIMER_RIGHT_10_TM=59.415
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.1000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=8.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=998
PRIMER_PAIR_11_PENALTY=3.929320
PRIMER_LEFT_11_PENALTY=0.559985
PRIMER_RIGHT_11_PENALTY=3.369335
PRIMER_LEFT_11_SEQUENCE=TAACAACAGCCCAGACGAGA
PRIMER_RIGHT_11_SEQUENCE=GTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_11=611,20
PRIMER_RIGHT_11=1606,20
PRIMER_LEFT_11_TM=59.440
PRIMER_RIGHT_11_TM=56.631
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=8.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=996
PRIMER_PAIR_12_PENALTY=3.966131
PRIMER_LEFT_12_PENALTY=1.076949
PRIMER_RIGHT_12_PENALTY=2.889182
PRIMER_LEFT_12_SEQUENCE=TTAACAACAGCCCAGACGAG
PRIMER_RIGHT_12_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_12=610,20
PRIMER_RIGHT_12=1608,21
PRIMER_LEFT_12_TM=58.923
PRIMER_RIGHT_12_TM=58.111
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=8.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=999
PRIMER_PAIR_13_PENALTY=4.020193
PRIMER_LEFT_13_PENALTY=0.682685
PRIMER_RIGHT_13_PENALTY=3.337508
PRIMER_LEFT_13_SEQUENCE=TTTAACAACAGCCCAGACGA
PRIMER_RIGHT_13_SEQUENCE=GAGTATGGGGTTTGTTGCTT
PRIMER_LEFT_13=609,20
PRIMER_RIGHT_13=1608,20
PRIMER_LEFT_13_TM=59.317
PRIMER_RIGHT_13_TM=56.662
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=8.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=1000
PRIMER_PAIR_14_PENALTY=4.072157
PRIMER_LEFT_14_PENALTY=1.487653
PRIMER_RIGHT_14_PENALTY=2.584504
PRIMER_LEFT_14_SEQUENCE=TCACGGCTCTACAATCGCTC
PRIMER_RIGHT_14_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_14=809,20
PRIMER_RIGHT_14=1608,22
PRIMER_LEFT_14_TM=61.488
PRIMER_RIGHT_14_TM=59.415
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=800
PRIMER_PAIR_15_PENALTY=4.131955
PRIMER_LEFT_15_PENALTY=0.306669
PRIMER_RIGHT_15_PENALTY=3.825286
PRIMER_LEFT_15_SEQUENCE=AACAACAGCCCAGACGAGAC
PRIMER_RIGHT_15_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_15=612,20
PRIMER_RIGHT_15=1530,20
PRIMER_LEFT_15_TM=60.307
PRIMER_RIGHT_15_TM=56.175
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=919
PRIMER_PAIR_16_PENALTY=4.271934
PRIMER_LEFT_16_PENALTY=0.934426
PRIMER_RIGHT_16_PENALTY=3.337508
PRIMER_LEFT_16_SEQUENCE=ACGGCTCTACAATCGCTCAA
PRIMER_RIGHT_16_SEQUENCE=GAGTATGGGGTTTGTTGCTT
PRIMER_LEFT_16=811,20
PRIMER_RIGHT_16=1608,20
PRIMER_LEFT_16_TM=60.934
PRIMER_RIGHT_16_TM=56.662
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=798
PRIMER_PAIR_17_PENALTY=4.303761
PRIMER_LEFT_17_PENALTY=0.934426
PRIMER_RIGHT_17_PENALTY=3.369335
PRIMER_LEFT_17_SEQUENCE=ACGGCTCTACAATCGCTCAA
PRIMER_RIGHT_17_SEQUENCE=GTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_17=811,20
PRIMER_RIGHT_17=1606,20
PRIMER_LEFT_17_TM=60.934
PRIMER_RIGHT_17_TM=56.631
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=796
PRIMER_PAIR_18_PENALTY=4.347594
PRIMER_LEFT_18_PENALTY=1.763090
PRIMER_RIGHT_18_PENALTY=2.584504
PRIMER_LEFT_18_SEQUENCE=CGCCAGAGCCAATTCTTCTT
PRIMER_RIGHT_18_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_18=724,20
PRIMER_RIGHT_18=1608,22
PRIMER_LEFT_18_TM=61.763
PRIMER_RIGHT_18_TM=59.415
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=885
PRIMER_PAIR_19_PENALTY=4.376835
PRIMER_LEFT_19_PENALTY=1.487653
PRIMER_RIGHT_19_PENALTY=2.889182
PRIMER_LEFT_19_SEQUENCE=TCACGGCTCTACAATCGCTC
PRIMER_RIGHT_19_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_19=809,20
PRIMER_RIGHT_19=1608,21
PRIMER_LEFT_19_TM=61.488
PRIMER_RIGHT_19_TM=58.111
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=800
PRIMER_PAIR_20_PENALTY=4.385271
PRIMER_LEFT_20_PENALTY=0.559985
PRIMER_RIGHT_20_PENALTY=3.825286
PRIMER_LEFT_20_SEQUENCE=TAACAACAGCCCAGACGAGA
PRIMER_RIGHT_20_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_20=611,20
PRIMER_RIGHT_20=1530,20
PRIMER_LEFT_20_TM=59.440
PRIMER_RIGHT_20_TM=56.175
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=920
PRIMER_PAIR_21_PENALTY=4.396210
PRIMER_LEFT_21_PENALTY=1.811706
PRIMER_RIGHT_21_PENALTY=2.584504
PRIMER_LEFT_21_SEQUENCE=TTAACAACAGCCCAGACGAGA
PRIMER_RIGHT_21_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_21=610,21
PRIMER_RIGHT_21=1608,22
PRIMER_LEFT_21_TM=60.812
PRIMER_RIGHT_21_TM=59.415
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=8.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=999
PRIMER_PAIR_22_PENALTY=4.484520
PRIMER_LEFT_22_PENALTY=1.900016
PRIMER_RIGHT_22_PENALTY=2.584504
PRIMER_LEFT_22_SEQUENCE=ACAACAGCCCAGACGAGACA
PRIMER_RIGHT_22_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_22=613,20
PRIMER_RIGHT_22=1608,22
PRIMER_LEFT_22_TM=61.900
PRIMER_RIGHT_22_TM=59.415
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=996
PRIMER_PAIR_23_PENALTY=4.507971
PRIMER_LEFT_23_PENALTY=0.682685
PRIMER_RIGHT_23_PENALTY=3.825286
PRIMER_LEFT_23_SEQUENCE=TTTAACAACAGCCCAGACGA
PRIMER_RIGHT_23_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_23=609,20
PRIMER_RIGHT_23=1530,20
PRIMER_LEFT_23_TM=59.317
PRIMER_RIGHT_23_TM=56.175
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=922
PRIMER_PAIR_24_PENALTY=4.611991
PRIMER_LEFT_24_PENALTY=2.027487
PRIMER_RIGHT_24_PENALTY=2.584504
PRIMER_LEFT_24_SEQUENCE=CAACAGCCCAGACGAGACAG
PRIMER_RIGHT_24_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_24=614,20
PRIMER_RIGHT_24=1608,22
PRIMER_LEFT_24_TM=62.027
PRIMER_RIGHT_24_TM=59.415
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=995
PRIMER_PAIR_25_PENALTY=4.652272
PRIMER_LEFT_25_PENALTY=1.763090
PRIMER_RIGHT_25_PENALTY=2.889182
PRIMER_LEFT_25_SEQUENCE=CGCCAGAGCCAATTCTTCTT
PRIMER_RIGHT_25_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_25=724,20
PRIMER_RIGHT_25=1608,21
PRIMER_LEFT_25_TM=61.763
PRIMER_RIGHT_25_TM=58.111
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=885
PRIMER_PAIR_26_PENALTY=4.664137
PRIMER_LEFT_26_PENALTY=1.774955
PRIMER_RIGHT_26_PENALTY=2.889182
PRIMER_LEFT_26_SEQUENCE=GCGAAAAGCTGGATACCATAA
PRIMER_RIGHT_26_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_26=833,21
PRIMER_RIGHT_26=1608,21
PRIMER_LEFT_26_TM=59.225
PRIMER_RIGHT_26_TM=58.111
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.2000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=776
PRIMER_PAIR_27_PENALTY=4.675054
PRIMER_LEFT_27_PENALTY=1.305719
PRIMER_RIGHT_27_PENALTY=3.369335
PRIMER_LEFT_27_SEQUENCE=TAACAACAGCCCAGACGAGAC
PRIMER_RIGHT_27_SEQUENCE=GTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_27=611,21
PRIMER_RIGHT_27=1606,20
PRIMER_LEFT_27_TM=60.306
PRIMER_RIGHT_27_TM=56.631
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.1000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=8.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=996
PRIMER_PAIR_28_PENALTY=4.697059
PRIMER_LEFT_28_PENALTY=0.934426
PRIMER_RIGHT_28_PENALTY=3.762633
PRIMER_LEFT_28_SEQUENCE=ACGGCTCTACAATCGCTCAA
PRIMER_RIGHT_28_SEQUENCE=AGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_28=811,20
PRIMER_RIGHT_28=1607,20
PRIMER_LEFT_28_TM=60.934
PRIMER_RIGHT_28_TM=56.237
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=797
PRIMER_PAIR_29_PENALTY=4.759712
PRIMER_LEFT_29_PENALTY=0.934426
PRIMER_RIGHT_29_PENALTY=3.825286
PRIMER_LEFT_29_SEQUENCE=ACGGCTCTACAATCGCTCAA
PRIMER_RIGHT_29_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_29=811,20
PRIMER_RIGHT_29=1530,20
PRIMER_LEFT_29_TM=60.934
PRIMER_RIGHT_29_TM=56.175
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=720
PRIMER_PAIR_30_PENALTY=4.789198
PRIMER_LEFT_30_PENALTY=1.900016
PRIMER_RIGHT_30_PENALTY=2.889182
PRIMER_LEFT_30_SEQUENCE=ACAACAGCCCAGACGAGACA
PRIMER_RIGHT_30_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_30=613,20
PRIMER_RIGHT_30=1608,21
PRIMER_LEFT_30_TM=61.900
PRIMER_RIGHT_30_TM=58.111
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=7.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=996
PRIMER_PAIR_31_PENALTY=4.825161
PRIMER_LEFT_31_PENALTY=1.487653
PRIMER_RIGHT_31_PENALTY=3.337508
PRIMER_LEFT_31_SEQUENCE=TCACGGCTCTACAATCGCTC
PRIMER_RIGHT_31_SEQUENCE=GAGTATGGGGTTTGTTGCTT
PRIMER_LEFT_31=809,20
PRIMER_RIGHT_31=1608,20
PRIMER_LEFT_31_TM=61.488
PRIMER_RIGHT_31_TM=56.662
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.9000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=800
PRIMER_PAIR_32_PENALTY=4.856988
PRIMER_LEFT_32_PENALTY=1.487653
PRIMER_RIGHT_32_PENALTY=3.369335
PRIMER_LEFT_32_SEQUENCE=TCACGGCTCTACAATCGCTC
PRIMER_RIGHT_32_SEQUENCE=GTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_32=809,20
PRIMER_RIGHT_32=1606,20
PRIMER_LEFT_32_TM=61.488
PRIMER_RIGHT_32_TM=56.631
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.9000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=798
PRIMER_PAIR_33_PENALTY=4.902235
PRIMER_LEFT_33_PENALTY=1.076949
PRIMER_RIGHT_33_PENALTY=3.825286
PRIMER_LEFT_33_SEQUENCE=TTAACAACAGCCCAGACGAG
PRIMER_RIGHT_33_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_33=610,20
PRIMER_RIGHT_33=1530,20
PRIMER_LEFT_33_TM=58.923
PRIMER_RIGHT_33_TM=56.175
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=921
PRIMER_PAIR_34_PENALTY=4.916669
PRIMER_LEFT_34_PENALTY=2.027487
PRIMER_RIGHT_34_PENALTY=2.889182
PRIMER_LEFT_34_SEQUENCE=CAACAGCCCAGACGAGACAG
PRIMER_RIGHT_34_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_34=614,20
PRIMER_RIGHT_34=1608,21
PRIMER_LEFT_34_TM=62.027
PRIMER_RIGHT_34_TM=58.111
PRIMER_LEFT_34_GC_PERCENT=60.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=995
PRIMER_PAIR_35_PENALTY=4.986966
PRIMER_LEFT_35_PENALTY=2.402462
PRIMER_RIGHT_35_PENALTY=2.584504
PRIMER_LEFT_35_SEQUENCE=TTCAGTCGTGATGATTCAACCT
PRIMER_RIGHT_35_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_35=674,22
PRIMER_RIGHT_35=1608,22
PRIMER_LEFT_35_TM=59.598
PRIMER_RIGHT_35_TM=59.415
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=7.3000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=935
PRIMER_PAIR_36_PENALTY=5.048978
PRIMER_LEFT_36_PENALTY=2.464474
PRIMER_RIGHT_36_PENALTY=2.584504
PRIMER_LEFT_36_SEQUENCE=CACGGCTCTACAATCGCTCA
PRIMER_RIGHT_36_SEQUENCE=GAGTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_36=810,20
PRIMER_RIGHT_36=1608,22
PRIMER_LEFT_36_TM=62.464
PRIMER_RIGHT_36_TM=59.415
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=799
PRIMER_PAIR_37_PENALTY=5.051859
PRIMER_LEFT_37_PENALTY=1.774955
PRIMER_RIGHT_37_PENALTY=3.276904
PRIMER_LEFT_37_SEQUENCE=GCGAAAAGCTGGATACCATAA
PRIMER_RIGHT_37_SEQUENCE=GGAGAGCAAGTTACTAAACGCA
PRIMER_LEFT_37=833,21
PRIMER_RIGHT_37=1821,22
PRIMER_LEFT_37_TM=59.225
PRIMER_RIGHT_37_TM=58.723
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.2000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=989
PRIMER_PAIR_38_PENALTY=5.100598
PRIMER_LEFT_38_PENALTY=1.763090
PRIMER_RIGHT_38_PENALTY=3.337508
PRIMER_LEFT_38_SEQUENCE=CGCCAGAGCCAATTCTTCTT
PRIMER_RIGHT_38_SEQUENCE=GAGTATGGGGTTTGTTGCTT
PRIMER_LEFT_38=724,20
PRIMER_RIGHT_38=1608,20
PRIMER_LEFT_38_TM=61.763
PRIMER_RIGHT_38_TM=56.662
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=885
PRIMER_PAIR_39_PENALTY=5.112463
PRIMER_LEFT_39_PENALTY=1.774955
PRIMER_RIGHT_39_PENALTY=3.337508
PRIMER_LEFT_39_SEQUENCE=GCGAAAAGCTGGATACCATAA
PRIMER_RIGHT_39_SEQUENCE=GAGTATGGGGTTTGTTGCTT
PRIMER_LEFT_39=833,21
PRIMER_RIGHT_39=1608,20
PRIMER_LEFT_39_TM=59.225
PRIMER_RIGHT_39_TM=56.662
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.2000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=776
PRIMER_PAIR_40_PENALTY=5.115569
PRIMER_LEFT_40_PENALTY=1.290283
PRIMER_RIGHT_40_PENALTY=3.825286
PRIMER_LEFT_40_SEQUENCE=TTTAACAACAGCCCAGACGAG
PRIMER_RIGHT_40_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_40=609,21
PRIMER_RIGHT_40=1530,20
PRIMER_LEFT_40_TM=60.290
PRIMER_RIGHT_40_TM=56.175
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=922
PRIMER_PAIR_41_PENALTY=5.115569
PRIMER_LEFT_41_PENALTY=1.290283
PRIMER_RIGHT_41_PENALTY=3.825286
PRIMER_LEFT_41_SEQUENCE=CTTTAACAACAGCCCAGACGA
PRIMER_RIGHT_41_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_41=608,21
PRIMER_RIGHT_41=1530,20
PRIMER_LEFT_41_TM=60.290
PRIMER_RIGHT_41_TM=56.175
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=923
PRIMER_PAIR_42_PENALTY=5.131005
PRIMER_LEFT_42_PENALTY=1.305719
PRIMER_RIGHT_42_PENALTY=3.825286
PRIMER_LEFT_42_SEQUENCE=TAACAACAGCCCAGACGAGAC
PRIMER_RIGHT_42_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_42=611,21
PRIMER_RIGHT_42=1530,20
PRIMER_LEFT_42_TM=60.306
PRIMER_RIGHT_42_TM=56.175
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.1000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=920
PRIMER_PAIR_43_PENALTY=5.132426
PRIMER_LEFT_43_PENALTY=1.763090
PRIMER_RIGHT_43_PENALTY=3.369335
PRIMER_LEFT_43_SEQUENCE=CGCCAGAGCCAATTCTTCTT
PRIMER_RIGHT_43_SEQUENCE=GTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_43=724,20
PRIMER_RIGHT_43=1606,20
PRIMER_LEFT_43_TM=61.763
PRIMER_RIGHT_43_TM=56.631
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.3000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=883
PRIMER_PAIR_44_PENALTY=5.143978
PRIMER_LEFT_44_PENALTY=1.774955
PRIMER_RIGHT_44_PENALTY=3.369023
PRIMER_LEFT_44_SEQUENCE=GCGAAAAGCTGGATACCATAA
PRIMER_RIGHT_44_SEQUENCE=GGAGAGCAAGTTACTAAACGCAG
PRIMER_LEFT_44=833,21
PRIMER_RIGHT_44=1821,23
PRIMER_LEFT_44_TM=59.225
PRIMER_RIGHT_44_TM=59.631
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=47.826
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.2000
PRIMER_RIGHT_44_END_STABILITY=10.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=989
PRIMER_PAIR_45_PENALTY=5.237524
PRIMER_LEFT_45_PENALTY=1.900016
PRIMER_RIGHT_45_PENALTY=3.337508
PRIMER_LEFT_45_SEQUENCE=ACAACAGCCCAGACGAGACA
PRIMER_RIGHT_45_SEQUENCE=GAGTATGGGGTTTGTTGCTT
PRIMER_LEFT_45=613,20
PRIMER_RIGHT_45=1608,20
PRIMER_LEFT_45_TM=61.900
PRIMER_RIGHT_45_TM=56.662
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=996
PRIMER_PAIR_46_PENALTY=5.250286
PRIMER_LEFT_46_PENALTY=1.487653
PRIMER_RIGHT_46_PENALTY=3.762633
PRIMER_LEFT_46_SEQUENCE=TCACGGCTCTACAATCGCTC
PRIMER_RIGHT_46_SEQUENCE=AGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_46=809,20
PRIMER_RIGHT_46=1607,20
PRIMER_LEFT_46_TM=61.488
PRIMER_RIGHT_46_TM=56.237
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=799
PRIMER_PAIR_47_PENALTY=5.269351
PRIMER_LEFT_47_PENALTY=1.900016
PRIMER_RIGHT_47_PENALTY=3.369335
PRIMER_LEFT_47_SEQUENCE=ACAACAGCCCAGACGAGACA
PRIMER_RIGHT_47_SEQUENCE=GTATGGGGTTTGTTGCTTTT
PRIMER_LEFT_47=613,20
PRIMER_RIGHT_47=1606,20
PRIMER_LEFT_47_TM=61.900
PRIMER_RIGHT_47_TM=56.631
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=7.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=994
PRIMER_PAIR_48_PENALTY=5.280436
PRIMER_LEFT_48_PENALTY=1.455150
PRIMER_RIGHT_48_PENALTY=3.825286
PRIMER_LEFT_48_SEQUENCE=CTTTTGGGCTCATTTGGCTT
PRIMER_RIGHT_48_SEQUENCE=GGTTTTGGTGTTTTTCCTTC
PRIMER_LEFT_48=574,20
PRIMER_RIGHT_48=1530,20
PRIMER_LEFT_48_TM=61.455
PRIMER_RIGHT_48_TM=56.175
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=2.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=957
PRIMER_PAIR_49_PENALTY=5.291644
PRIMER_LEFT_49_PENALTY=2.402462
PRIMER_RIGHT_49_PENALTY=2.889182
PRIMER_LEFT_49_SEQUENCE=TTCAGTCGTGATGATTCAACCT
PRIMER_RIGHT_49_SEQUENCE=GAGTATGGGGTTTGTTGCTTT
PRIMER_LEFT_49=674,22
PRIMER_RIGHT_49=1608,21
PRIMER_LEFT_49_TM=59.598
PRIMER_RIGHT_49_TM=58.111
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=935
=
SEQUENCE_ID=MAGIC.105_4_10887982_10888068_wil-2_po-0_35166_10501_TRUE
SEQUENCE_TARGET=950,186
SEQUENCE_TEMPLATE=TTCACAAGCTACACACAAGCCTCTCTTTCGAAACGGAGCTTCAAAGTCCGATATCANGTGGGAGGACATCATGGAAGCTACANATAATCTAAGNGAGGAGTTCATGATTGGATCAGGNGGCTCTGGGAAGGTTTACAAGGCGGAGCTTGAAAATGGTGAGACTGTTGCNGTGAAAAAGATTCTCTGGAAGGATGATCTCATGTCAAACAAGAGCTTCAGNAGAGAAGTTAAGACGCTTGGAAGAATNAGGCATAGGCATCTGGTTAAGCTAATGGGTTACTGCAGCAGCAAGTCAGAAGGATTGAATCTGTTGATATATGAGTACATGAAGAACGGAAGCATCTGGGATTGGCTTCATGAGGANAAACCNGTGCTCGAAAAANNNANGAAGCTTTTAGACTGGGAAGCAAGATTGAGAATAGCAGTAGGATTGGCTCAAGGAGTAGAGTACCTTCATCATGACTGNGTTCCTCCTATCGTNCACCGTGATATNAAATCCAGTAATGTGCTCCTCGATTCAAACATGGAAGCNCATTTAGGAGATTTCGGTCTTGCCAAGGTCTTAACTGAGAACTGTGATACCAACACAGATTCAAATACCTGGTTTGCATGCTCTTATGGCTACATTGCTCCAGGTACTCATCTTCTCTTTCTTAGNTTTCTTGCATATTCACTGTTTTAATATCTAAAGATTTGAAATTGTGTNTCTAGAGTATGCTTACTCGTTGAAGGCGACTGAGAAGAGNGACGTTTANAGTATGGGGATCGTGTTGATGGAGATTGTGACTGGGAAAATGCCAACCGACTCAGTGTTTGGTGCAGAGATGGACATGGTGAGATGGGTTGAAACACATNTTGANGTAGCGGGTTCTGCGAGAGATAANCTCATAGATCCAAAGCTTAAGCCACTNNTGCCATTTGAGGAANACGCAGCTTNTCAGGTGCTTGAGATTGCACTTCAGTGCACAAAAACTTCTCCCCAAGAGAGACCATCTTCTAGNCAAGCTTGTGATAGTCTTCTCCATGTCTACAACAACAGAACGGCCGGTTANAAGAAGCTGTAAAAAAACTGACANCTGCAAATGTNTTTGGAGACATGAAAACTGCCNACTTTANNNTTTTTNTTCTGAAATTCTGTTTCTGCTTTGTNTAGATGACATGGNNNNTTTCAGTACAAAGATTGTTTCGAACTTGATCCATCTGCTANNAAAANACTTANTGTCNNTTGCATGTACTTGTAATACAACTTAAACCAAANTTTAAACANANTGATTCTGATTCTGATTCTGAAACTCTNTTCTCATAACAGAAGGTAGATTGCAAGGTTTATCATTTNGCCATTATTAACCAGATACAAACAGATTCAGNTTGTGAAAACACAAATCTTTAGGTTCATATTCGAAAACAGTGAATGAAAAGAACTCCAGAGCTGAAGTNAANGAGAAGGAGAAGAAACGCTTNTACAGGAAAGATCAGACAAAACAAGCACTTGAGATGAGTTCAGGGGAAGATATTTCTTTTCTGAAGACTTCAAAGATCACGCAAAANTAATCCCGATCTTCATCTGCATTACAAANACAGAANGAAAAGTTTGAATATTCNAANTNATTCAATCCAACAAAACNAGAAATGNAATACNNCAANTTCTNCCAGNCCCTNNCNAATATGCAATANCCAATGGNGATNAACAAACAGAGATTNTTGAACTNANCANANGTCACAANATTTATCNAAGAAGAGTTTNNGAAAGCTTAGCAACTNACNANCTAAAACGAGACACGTNTACATATGTGGTTCCCTTCAAATTCGCATCTAGTTTATATGTACTGGCACCTTGCAANNTATCTAACTCTAGAACCAAAGAACTAGNCGAAAGTATCTATCTTTCCAGACTCATTGAGATCATGTTCAAAGGAATAATTGTATTGCTCAAACTGTCTTAACTTTTNTAAGAGTGCCTGACTAAACAGATTTGCAACAAGCTAAGCCTGCANAGAGATTCGCTNAATTGAAAAACTTCCAAANGATNAAACCTTGAGCNAAGTTTCATTGCGGAGAATTCANTTACCATTGCAACCATTTCATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.410966
PRIMER_LEFT_0_PENALTY=0.056085
PRIMER_RIGHT_0_PENALTY=0.354881
PRIMER_LEFT_0_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_0_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_0=824,20
PRIMER_RIGHT_0=1813,20
PRIMER_LEFT_0_TM=60.056
PRIMER_RIGHT_0_TM=59.645
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=990
PRIMER_PAIR_1_PENALTY=0.476275
PRIMER_LEFT_1_PENALTY=0.121394
PRIMER_RIGHT_1_PENALTY=0.354881
PRIMER_LEFT_1_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_1_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_1=816,20
PRIMER_RIGHT_1=1813,20
PRIMER_LEFT_1_TM=60.121
PRIMER_RIGHT_1_TM=59.645
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=998
PRIMER_PAIR_2_PENALTY=0.546372
PRIMER_LEFT_2_PENALTY=0.056085
PRIMER_RIGHT_2_PENALTY=0.490287
PRIMER_LEFT_2_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_2_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_2=824,20
PRIMER_RIGHT_2=1812,20
PRIMER_LEFT_2_TM=60.056
PRIMER_RIGHT_2_TM=59.510
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=989
PRIMER_PAIR_3_PENALTY=0.611681
PRIMER_LEFT_3_PENALTY=0.121394
PRIMER_RIGHT_3_PENALTY=0.490287
PRIMER_LEFT_3_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_3_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_3=816,20
PRIMER_RIGHT_3=1812,20
PRIMER_LEFT_3_TM=60.121
PRIMER_RIGHT_3_TM=59.510
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=997
PRIMER_PAIR_4_PENALTY=0.919136
PRIMER_LEFT_4_PENALTY=0.056085
PRIMER_RIGHT_4_PENALTY=0.863051
PRIMER_LEFT_4_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_4_SEQUENCE=CGAATTTGAAGGGAACCACA
PRIMER_LEFT_4=824,20
PRIMER_RIGHT_4=1808,20
PRIMER_LEFT_4_TM=60.056
PRIMER_RIGHT_4_TM=60.863
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=985
PRIMER_PAIR_5_PENALTY=0.984445
PRIMER_LEFT_5_PENALTY=0.121394
PRIMER_RIGHT_5_PENALTY=0.863051
PRIMER_LEFT_5_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_5_SEQUENCE=CGAATTTGAAGGGAACCACA
PRIMER_LEFT_5=816,20
PRIMER_RIGHT_5=1808,20
PRIMER_LEFT_5_TM=60.121
PRIMER_RIGHT_5_TM=60.863
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=993
PRIMER_PAIR_6_PENALTY=1.036583
PRIMER_LEFT_6_PENALTY=0.681702
PRIMER_RIGHT_6_PENALTY=0.354881
PRIMER_LEFT_6_SEQUENCE=GACATGGTGAGATGGGTTGA
PRIMER_RIGHT_6_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_6=827,20
PRIMER_RIGHT_6=1813,20
PRIMER_LEFT_6_TM=59.318
PRIMER_RIGHT_6_TM=59.645
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=987
PRIMER_PAIR_7_PENALTY=1.143273
PRIMER_LEFT_7_PENALTY=0.788392
PRIMER_RIGHT_7_PENALTY=0.354881
PRIMER_LEFT_7_SEQUENCE=GCAGAGATGGACATGGTGAG
PRIMER_RIGHT_7_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_7=818,20
PRIMER_RIGHT_7=1813,20
PRIMER_LEFT_7_TM=59.212
PRIMER_RIGHT_7_TM=59.645
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=996
PRIMER_PAIR_8_PENALTY=1.171989
PRIMER_LEFT_8_PENALTY=0.681702
PRIMER_RIGHT_8_PENALTY=0.490287
PRIMER_LEFT_8_SEQUENCE=GACATGGTGAGATGGGTTGA
PRIMER_RIGHT_8_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_8=827,20
PRIMER_RIGHT_8=1812,20
PRIMER_LEFT_8_TM=59.318
PRIMER_RIGHT_8_TM=59.510
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=986
PRIMER_PAIR_9_PENALTY=1.180986
PRIMER_LEFT_9_PENALTY=0.317935
PRIMER_RIGHT_9_PENALTY=0.863051
PRIMER_LEFT_9_SEQUENCE=GTGTTTGGTGCAGAGATGGA
PRIMER_RIGHT_9_SEQUENCE=CGAATTTGAAGGGAACCACA
PRIMER_LEFT_9=809,20
PRIMER_RIGHT_9=1808,20
PRIMER_LEFT_9_TM=59.682
PRIMER_RIGHT_9_TM=60.863
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=1000
PRIMER_PAIR_10_PENALTY=1.278679
PRIMER_LEFT_10_PENALTY=0.788392
PRIMER_RIGHT_10_PENALTY=0.490287
PRIMER_LEFT_10_SEQUENCE=GCAGAGATGGACATGGTGAG
PRIMER_RIGHT_10_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_10=818,20
PRIMER_RIGHT_10=1812,20
PRIMER_LEFT_10_TM=59.212
PRIMER_RIGHT_10_TM=59.510
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=995
PRIMER_PAIR_11_PENALTY=1.385268
PRIMER_LEFT_11_PENALTY=0.056085
PRIMER_RIGHT_11_PENALTY=1.329183
PRIMER_LEFT_11_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_11_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_11=824,20
PRIMER_RIGHT_11=1571,20
PRIMER_LEFT_11_TM=60.056
PRIMER_RIGHT_11_TM=58.671
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=748
PRIMER_PAIR_12_PENALTY=1.450577
PRIMER_LEFT_12_PENALTY=0.121394
PRIMER_RIGHT_12_PENALTY=1.329183
PRIMER_LEFT_12_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_12_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_12=816,20
PRIMER_RIGHT_12=1571,20
PRIMER_LEFT_12_TM=60.121
PRIMER_RIGHT_12_TM=58.671
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=11.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=756
PRIMER_PAIR_13_PENALTY=1.501394
PRIMER_LEFT_13_PENALTY=0.056085
PRIMER_RIGHT_13_PENALTY=1.445309
PRIMER_LEFT_13_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_13_SEQUENCE=AGATGCGAATTTGAAGGGAAC
PRIMER_LEFT_13=824,20
PRIMER_RIGHT_13=1813,21
PRIMER_LEFT_13_TM=60.056
PRIMER_RIGHT_13_TM=60.445
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=990
PRIMER_PAIR_14_PENALTY=1.513951
PRIMER_LEFT_14_PENALTY=0.056085
PRIMER_RIGHT_14_PENALTY=1.457866
PRIMER_LEFT_14_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_14_SEQUENCE=GTAATGCAGATGAAGATCGGG
PRIMER_LEFT_14=824,20
PRIMER_RIGHT_14=1572,21
PRIMER_LEFT_14_TM=60.056
PRIMER_RIGHT_14_TM=59.542
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=11.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=749
PRIMER_PAIR_15_PENALTY=1.534522
PRIMER_LEFT_15_PENALTY=1.179641
PRIMER_RIGHT_15_PENALTY=0.354881
PRIMER_LEFT_15_SEQUENCE=GATGGACATGGTGAGATGGG
PRIMER_RIGHT_15_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_15=823,20
PRIMER_RIGHT_15=1813,20
PRIMER_LEFT_15_TM=61.180
PRIMER_RIGHT_15_TM=59.645
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=991
PRIMER_PAIR_16_PENALTY=1.544753
PRIMER_LEFT_16_PENALTY=0.681702
PRIMER_RIGHT_16_PENALTY=0.863051
PRIMER_LEFT_16_SEQUENCE=GACATGGTGAGATGGGTTGA
PRIMER_RIGHT_16_SEQUENCE=CGAATTTGAAGGGAACCACA
PRIMER_LEFT_16=827,20
PRIMER_RIGHT_16=1808,20
PRIMER_LEFT_16_TM=59.318
PRIMER_RIGHT_16_TM=60.863
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=982
PRIMER_PAIR_17_PENALTY=1.564025
PRIMER_LEFT_17_PENALTY=1.209144
PRIMER_RIGHT_17_PENALTY=0.354881
PRIMER_LEFT_17_SEQUENCE=GGACATGGTGAGATGGGTTG
PRIMER_RIGHT_17_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_17=826,20
PRIMER_RIGHT_17=1813,20
PRIMER_LEFT_17_TM=61.209
PRIMER_RIGHT_17_TM=59.645
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=988
PRIMER_PAIR_18_PENALTY=1.564025
PRIMER_LEFT_18_PENALTY=1.209144
PRIMER_RIGHT_18_PENALTY=0.354881
PRIMER_LEFT_18_SEQUENCE=TGGACATGGTGAGATGGGTT
PRIMER_RIGHT_18_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_18=825,20
PRIMER_RIGHT_18=1813,20
PRIMER_LEFT_18_TM=61.209
PRIMER_RIGHT_18_TM=59.645
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=989
PRIMER_PAIR_19_PENALTY=1.566703
PRIMER_LEFT_19_PENALTY=0.121394
PRIMER_RIGHT_19_PENALTY=1.445309
PRIMER_LEFT_19_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_19_SEQUENCE=AGATGCGAATTTGAAGGGAAC
PRIMER_LEFT_19=816,20
PRIMER_RIGHT_19=1813,21
PRIMER_LEFT_19_TM=60.121
PRIMER_RIGHT_19_TM=60.445
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=998
PRIMER_PAIR_20_PENALTY=1.579260
PRIMER_LEFT_20_PENALTY=0.121394
PRIMER_RIGHT_20_PENALTY=1.457866
PRIMER_LEFT_20_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_20_SEQUENCE=GTAATGCAGATGAAGATCGGG
PRIMER_LEFT_20=816,20
PRIMER_RIGHT_20=1572,21
PRIMER_LEFT_20_TM=60.121
PRIMER_RIGHT_20_TM=59.542
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=757
PRIMER_PAIR_21_PENALTY=1.641106
PRIMER_LEFT_21_PENALTY=0.311923
PRIMER_RIGHT_21_PENALTY=1.329183
PRIMER_LEFT_21_SEQUENCE=TGGTTTGCATGCTCTTATGG
PRIMER_RIGHT_21_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_21=601,20
PRIMER_RIGHT_21=1571,20
PRIMER_LEFT_21_TM=59.688
PRIMER_RIGHT_21_TM=58.671
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.4000
PRIMER_RIGHT_21_END_STABILITY=11.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=971
PRIMER_PAIR_22_PENALTY=1.647118
PRIMER_LEFT_22_PENALTY=0.317935
PRIMER_RIGHT_22_PENALTY=1.329183
PRIMER_LEFT_22_SEQUENCE=GTGTTTGGTGCAGAGATGGA
PRIMER_RIGHT_22_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_22=809,20
PRIMER_RIGHT_22=1571,20
PRIMER_LEFT_22_TM=59.682
PRIMER_RIGHT_22_TM=58.671
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=11.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=763
PRIMER_PAIR_23_PENALTY=1.649566
PRIMER_LEFT_23_PENALTY=1.294685
PRIMER_RIGHT_23_PENALTY=0.354881
PRIMER_LEFT_23_SEQUENCE=TGCAGAGATGGACATGGTGA
PRIMER_RIGHT_23_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_23=817,20
PRIMER_RIGHT_23=1813,20
PRIMER_LEFT_23_TM=61.295
PRIMER_RIGHT_23_TM=59.645
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=997
PRIMER_PAIR_24_PENALTY=1.651443
PRIMER_LEFT_24_PENALTY=0.788392
PRIMER_RIGHT_24_PENALTY=0.863051
PRIMER_LEFT_24_SEQUENCE=GCAGAGATGGACATGGTGAG
PRIMER_RIGHT_24_SEQUENCE=CGAATTTGAAGGGAACCACA
PRIMER_LEFT_24=818,20
PRIMER_RIGHT_24=1808,20
PRIMER_LEFT_24_TM=59.212
PRIMER_RIGHT_24_TM=60.863
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=991
PRIMER_PAIR_25_PENALTY=1.669928
PRIMER_LEFT_25_PENALTY=1.179641
PRIMER_RIGHT_25_PENALTY=0.490287
PRIMER_LEFT_25_SEQUENCE=GATGGACATGGTGAGATGGG
PRIMER_RIGHT_25_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_25=823,20
PRIMER_RIGHT_25=1812,20
PRIMER_LEFT_25_TM=61.180
PRIMER_RIGHT_25_TM=59.510
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=990
PRIMER_PAIR_26_PENALTY=1.690950
PRIMER_LEFT_26_PENALTY=0.361767
PRIMER_RIGHT_26_PENALTY=1.329183
PRIMER_LEFT_26_SEQUENCE=GTGACTGGGAAAATGCCAAC
PRIMER_RIGHT_26_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_26=782,20
PRIMER_RIGHT_26=1571,20
PRIMER_LEFT_26_TM=60.362
PRIMER_RIGHT_26_TM=58.671
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=11.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=790
PRIMER_PAIR_27_PENALTY=1.696876
PRIMER_LEFT_27_PENALTY=1.341995
PRIMER_RIGHT_27_PENALTY=0.354881
PRIMER_LEFT_27_SEQUENCE=GAGATGGACATGGTGAGATGG
PRIMER_RIGHT_27_SEQUENCE=AGATGCGAATTTGAAGGGAA
PRIMER_LEFT_27=821,21
PRIMER_RIGHT_27=1813,20
PRIMER_LEFT_27_TM=60.342
PRIMER_RIGHT_27_TM=59.645
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=993
PRIMER_PAIR_28_PENALTY=1.699431
PRIMER_LEFT_28_PENALTY=1.209144
PRIMER_RIGHT_28_PENALTY=0.490287
PRIMER_LEFT_28_SEQUENCE=GGACATGGTGAGATGGGTTG
PRIMER_RIGHT_28_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_28=826,20
PRIMER_RIGHT_28=1812,20
PRIMER_LEFT_28_TM=61.209
PRIMER_RIGHT_28_TM=59.510
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=987
PRIMER_PAIR_29_PENALTY=1.699431
PRIMER_LEFT_29_PENALTY=1.209144
PRIMER_RIGHT_29_PENALTY=0.490287
PRIMER_LEFT_29_SEQUENCE=TGGACATGGTGAGATGGGTT
PRIMER_RIGHT_29_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_29=825,20
PRIMER_RIGHT_29=1812,20
PRIMER_LEFT_29_TM=61.209
PRIMER_RIGHT_29_TM=59.510
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=988
PRIMER_PAIR_30_PENALTY=1.738603
PRIMER_LEFT_30_PENALTY=0.409420
PRIMER_RIGHT_30_PENALTY=1.329183
PRIMER_LEFT_30_SEQUENCE=TACTCGTTGAAGGCGACTGA
PRIMER_RIGHT_30_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_30=719,20
PRIMER_RIGHT_30=1571,20
PRIMER_LEFT_30_TM=59.591
PRIMER_RIGHT_30_TM=58.671
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=11.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=853
PRIMER_PAIR_31_PENALTY=1.747667
PRIMER_LEFT_31_PENALTY=0.056085
PRIMER_RIGHT_31_PENALTY=1.691582
PRIMER_LEFT_31_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_31_SEQUENCE=TAGATGCGAATTTGAAGGGA
PRIMER_LEFT_31=824,20
PRIMER_RIGHT_31=1814,20
PRIMER_LEFT_31_TM=60.056
PRIMER_RIGHT_31_TM=58.308
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=991
PRIMER_PAIR_32_PENALTY=1.750100
PRIMER_LEFT_32_PENALTY=0.056085
PRIMER_RIGHT_32_PENALTY=1.694015
PRIMER_LEFT_32_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_32_SEQUENCE=CTAGATGCGAATTTGAAGGGA
PRIMER_LEFT_32=824,20
PRIMER_RIGHT_32=1815,21
PRIMER_LEFT_32_TM=60.056
PRIMER_RIGHT_32_TM=59.306
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=992
PRIMER_PAIR_33_PENALTY=1.769789
PRIMER_LEFT_33_PENALTY=0.311923
PRIMER_RIGHT_33_PENALTY=1.457866
PRIMER_LEFT_33_SEQUENCE=TGGTTTGCATGCTCTTATGG
PRIMER_RIGHT_33_SEQUENCE=GTAATGCAGATGAAGATCGGG
PRIMER_LEFT_33=601,20
PRIMER_RIGHT_33=1572,21
PRIMER_LEFT_33_TM=59.688
PRIMER_RIGHT_33_TM=59.542
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.4000
PRIMER_RIGHT_33_END_STABILITY=11.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=972
PRIMER_PAIR_34_PENALTY=1.775801
PRIMER_LEFT_34_PENALTY=0.317935
PRIMER_RIGHT_34_PENALTY=1.457866
PRIMER_LEFT_34_SEQUENCE=GTGTTTGGTGCAGAGATGGA
PRIMER_RIGHT_34_SEQUENCE=GTAATGCAGATGAAGATCGGG
PRIMER_LEFT_34=809,20
PRIMER_RIGHT_34=1572,21
PRIMER_LEFT_34_TM=59.682
PRIMER_RIGHT_34_TM=59.542
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=11.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=764
PRIMER_PAIR_35_PENALTY=1.784972
PRIMER_LEFT_35_PENALTY=1.294685
PRIMER_RIGHT_35_PENALTY=0.490287
PRIMER_LEFT_35_SEQUENCE=TGCAGAGATGGACATGGTGA
PRIMER_RIGHT_35_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_35=817,20
PRIMER_RIGHT_35=1812,20
PRIMER_LEFT_35_TM=61.295
PRIMER_RIGHT_35_TM=59.510
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=996
PRIMER_PAIR_36_PENALTY=1.797669
PRIMER_LEFT_36_PENALTY=0.056085
PRIMER_RIGHT_36_PENALTY=1.741584
PRIMER_LEFT_36_SEQUENCE=ATGGACATGGTGAGATGGGT
PRIMER_RIGHT_36_SEQUENCE=GCGAATTTGAAGGGAACCAC
PRIMER_LEFT_36=824,20
PRIMER_RIGHT_36=1809,20
PRIMER_LEFT_36_TM=60.056
PRIMER_RIGHT_36_TM=61.742
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=986
PRIMER_PAIR_37_PENALTY=1.812976
PRIMER_LEFT_37_PENALTY=0.121394
PRIMER_RIGHT_37_PENALTY=1.691582
PRIMER_LEFT_37_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_37_SEQUENCE=TAGATGCGAATTTGAAGGGA
PRIMER_LEFT_37=816,20
PRIMER_RIGHT_37=1814,20
PRIMER_LEFT_37_TM=60.121
PRIMER_RIGHT_37_TM=58.308
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=999
PRIMER_PAIR_38_PENALTY=1.815409
PRIMER_LEFT_38_PENALTY=0.121394
PRIMER_RIGHT_38_PENALTY=1.694015
PRIMER_LEFT_38_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_38_SEQUENCE=CTAGATGCGAATTTGAAGGGA
PRIMER_LEFT_38=816,20
PRIMER_RIGHT_38=1815,21
PRIMER_LEFT_38_TM=60.121
PRIMER_RIGHT_38_TM=59.306
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=1000
PRIMER_PAIR_39_PENALTY=1.819633
PRIMER_LEFT_39_PENALTY=0.361767
PRIMER_RIGHT_39_PENALTY=1.457866
PRIMER_LEFT_39_SEQUENCE=GTGACTGGGAAAATGCCAAC
PRIMER_RIGHT_39_SEQUENCE=GTAATGCAGATGAAGATCGGG
PRIMER_LEFT_39=782,20
PRIMER_RIGHT_39=1572,21
PRIMER_LEFT_39_TM=60.362
PRIMER_RIGHT_39_TM=59.542
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=11.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=791
PRIMER_PAIR_40_PENALTY=1.832282
PRIMER_LEFT_40_PENALTY=1.341995
PRIMER_RIGHT_40_PENALTY=0.490287
PRIMER_LEFT_40_SEQUENCE=GAGATGGACATGGTGAGATGG
PRIMER_RIGHT_40_SEQUENCE=GATGCGAATTTGAAGGGAAC
PRIMER_LEFT_40=821,21
PRIMER_RIGHT_40=1812,20
PRIMER_LEFT_40_TM=60.342
PRIMER_RIGHT_40_TM=59.510
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=992
PRIMER_PAIR_41_PENALTY=1.851982
PRIMER_LEFT_41_PENALTY=0.121394
PRIMER_RIGHT_41_PENALTY=1.730588
PRIMER_LEFT_41_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_41_SEQUENCE=CTTCCCCTGAACTCATCTCAA
PRIMER_LEFT_41=816,20
PRIMER_RIGHT_41=1509,21
PRIMER_LEFT_41_TM=60.121
PRIMER_RIGHT_41_TM=59.269
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=694
PRIMER_PAIR_42_PENALTY=1.856353
PRIMER_LEFT_42_PENALTY=0.527169
PRIMER_RIGHT_42_PENALTY=1.329183
PRIMER_LEFT_42_SEQUENCE=TGATGGAGATTGTGACTGGG
PRIMER_RIGHT_42_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_42=771,20
PRIMER_RIGHT_42=1571,20
PRIMER_LEFT_42_TM=59.473
PRIMER_RIGHT_42_TM=58.671
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=11.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=801
PRIMER_PAIR_43_PENALTY=1.862978
PRIMER_LEFT_43_PENALTY=0.121394
PRIMER_RIGHT_43_PENALTY=1.741584
PRIMER_LEFT_43_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_43_SEQUENCE=GCGAATTTGAAGGGAACCAC
PRIMER_LEFT_43=816,20
PRIMER_RIGHT_43=1809,20
PRIMER_LEFT_43_TM=60.121
PRIMER_RIGHT_43_TM=61.742
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=994
PRIMER_PAIR_44_PENALTY=1.867286
PRIMER_LEFT_44_PENALTY=0.409420
PRIMER_RIGHT_44_PENALTY=1.457866
PRIMER_LEFT_44_SEQUENCE=TACTCGTTGAAGGCGACTGA
PRIMER_RIGHT_44_SEQUENCE=GTAATGCAGATGAAGATCGGG
PRIMER_LEFT_44=719,20
PRIMER_RIGHT_44=1572,21
PRIMER_LEFT_44_TM=59.591
PRIMER_RIGHT_44_TM=59.542
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=11.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=854
PRIMER_PAIR_45_PENALTY=1.920266
PRIMER_LEFT_45_PENALTY=0.121394
PRIMER_RIGHT_45_PENALTY=1.798872
PRIMER_LEFT_45_SEQUENCE=GTGCAGAGATGGACATGGTG
PRIMER_RIGHT_45_SEQUENCE=TTCCCCTGAACTCATCTCAA
PRIMER_LEFT_45=816,20
PRIMER_RIGHT_45=1508,20
PRIMER_LEFT_45_TM=60.121
PRIMER_RIGHT_45_TM=58.201
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=693
PRIMER_PAIR_46_PENALTY=1.985036
PRIMER_LEFT_46_PENALTY=0.527169
PRIMER_RIGHT_46_PENALTY=1.457866
PRIMER_LEFT_46_SEQUENCE=TGATGGAGATTGTGACTGGG
PRIMER_RIGHT_46_SEQUENCE=GTAATGCAGATGAAGATCGGG
PRIMER_LEFT_46=771,20
PRIMER_RIGHT_46=1572,21
PRIMER_LEFT_46_TM=59.473
PRIMER_RIGHT_46_TM=59.542
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=11.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=802
PRIMER_PAIR_47_PENALTY=2.010885
PRIMER_LEFT_47_PENALTY=0.681702
PRIMER_RIGHT_47_PENALTY=1.329183
PRIMER_LEFT_47_SEQUENCE=GACATGGTGAGATGGGTTGA
PRIMER_RIGHT_47_SEQUENCE=TAATGCAGATGAAGATCGGG
PRIMER_LEFT_47=827,20
PRIMER_RIGHT_47=1571,20
PRIMER_LEFT_47_TM=59.318
PRIMER_RIGHT_47_TM=58.671
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=11.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=745
PRIMER_PAIR_48_PENALTY=2.042511
PRIMER_LEFT_48_PENALTY=0.311923
PRIMER_RIGHT_48_PENALTY=1.730588
PRIMER_LEFT_48_SEQUENCE=TGGTTTGCATGCTCTTATGG
PRIMER_RIGHT_48_SEQUENCE=CTTCCCCTGAACTCATCTCAA
PRIMER_LEFT_48=601,20
PRIMER_RIGHT_48=1509,21
PRIMER_LEFT_48_TM=59.688
PRIMER_RIGHT_48_TM=59.269
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.4000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=909
PRIMER_PAIR_49_PENALTY=2.042692
PRIMER_LEFT_49_PENALTY=1.179641
PRIMER_RIGHT_49_PENALTY=0.863051
PRIMER_LEFT_49_SEQUENCE=GATGGACATGGTGAGATGGG
PRIMER_RIGHT_49_SEQUENCE=CGAATTTGAAGGGAACCACA
PRIMER_LEFT_49=823,20
PRIMER_RIGHT_49=1808,20
PRIMER_LEFT_49_TM=61.180
PRIMER_RIGHT_49_TM=60.863
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=986
=
SEQUENCE_ID=MAGIC.433_4_4234105_4234155_oy-0_wu-0_222186_86705_TRUE
SEQUENCE_TARGET=950,150
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_17945288_17945544_sf-2_kn-0_261726_91784_TRUE
SEQUENCE_TARGET=950,356
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAATACATACCANTTTCTNACTNTCGTCCACGGANANNNNNNTCTCTACAANGACTGTTTTGGTTTGAGAANNNNNNNNNNNNNNNNNNNNNNTGAATAGANTCTAAGTAGTCAATATTTTCTTANTAATGTTACNCGTCTCATNAANNTGGNTGTGGACTCTCTTTTAATTTTAGTNACTGCTCATTCNATNAAAANATAANAAACTNCAAGNTTATNNNNTNCTTAATNAAAATTTGAAANACNTTTGTATAAAGACAAATACTATTAGTANCANCATAACTCATTTNTTNCCNTATAAATAACAACTNATCTTNGNATTNTTNATCACTCAACAACAAACACAANAAANAATNATAAATTNNANTACTTCAAACATNTTTTTNCTTTNATNATGGCNATCAAAAANNTGATTTTTCTTCTATNGNTTNTATGNNTNNCAATGTCTNCAAATGCTCAATTACCTCAATTTCNGGCACCGTTTCNTTTTCCATTTNNANTNNNNNNNNNNNNNNNNNNNNTGNTATAACAAAATGTTTNTNATCCTTTATGAATATTNCANGGTGTATCGNAGAGNTTTCACAATCTATANTCANNGGAAAGTTTGGCAACATANNNNCNGNTTGTTNNAAAGCAATTTTNGAANCTNAAACCAANTGTATTCCNNAACTTCCATTCAANNNATTTTTTCCTCCTCTGCTAAANNAACAATGTTCAAAAANNNNTGGCNCAATTNNTCNTANNANAANNTAGAATTNAAAAANCAATAATNTAANNGTTGCNCTTTTTTCNANATTANAAAAATAANNTTAGGTTTACNNNTTGTATTCAAANTTTTCAAACATCTAATAATAGCATTATNNNAAAANAGNCNCNTTTTTGTNAACAGTCATNAAATAGTTCAAANTGNTAGCNATCTACATATTGNAAAAAGAAGCAATTCTTATGCAAATTCTANNAATATTGCTATTTTNATACTCANTAANTGGATATTCACATTNNAAANNNNTGAGCATCGTTNTNTAATATTCGTGGTNTGATTACAAGATCTATCTATGTAANTAAANTGAAAACGGANCAGNNATCCNCNNNNNNNNNNNNNNNNTNTNTGNNNNNTNNNTNTTTTTTTNNNANNAATNNNNNTTAATANTTNTTTTGCATNNTAGANTTACATATATCNAGACTTTTNANATCAAANTNAAACNGATAACAAATNGAANCGAANNTAACANATAATNNGTCTNATCCNTAATNTAATCTACATTTCCATCTTCTNTTTACACANTGANNTTTTAGATGATNAAAAANATAAAACNAACCAGAGTTTTTGTNGGTTCATACAANANACNNNNNNAAAANCAAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATTAAGAAATGGANAAAAAGAAGGAATANTAGNAANNNNNNGANTTCTGTAACGGCTAGTTCCAAAGTACCGTTNTTTTTTTNGTTTTAATTATTGTATTAAGGCACGCTCTGACACATTCACACTCANANANAGNGAGAAGAAAAGNNAAAAAAANNNTNNAAACTTTTGCTTNTACTCNATTCTCTNATTTAGGNNNNNNNNNNNNNTTTACACTATCTCGATTTGAGAAGNGAGAAGAGAAATCGCCATGAACATNTTCAAGAAGAAGACCACTCCCAAAGGTATNAAAAAGCTTNAAACTTTATCNCATTGTNTCTCTGAGATTNTGTTNNNNNNNNNNTTTTTTTCTTCATNTCTGGNGAAATCGCGAGGANTNGTTTNNNTATCTGTTGGGTAACANAAAANATGNNAGCTTTAAGTGTTCTTNGTGTTCAAAATTGAATCTTGTAGTGTGNGTNAGTGTGAATTTTTCTGGGTTCTNAGTTTCNANTNAANGNNNNNNTAGATAGATTCCTTAATTAGATGTGTAANTAAAGTNNNNNNNNNNNNNNNATTGNAAAATAGTNAGTTGNCNANANTTAGNTAACTTGTGNNNNNNNNAATTTTGCNTTTGATTCTNTTGAAAAANNTNTGANTTTTNNNTTCTAATNCTGCAGATGCTCTTCGTACTAGTAAGAGAGAAATGGCTGTTGCTACTCNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_9625340_9625664_zu-0_ct-1_12113_853901_TRUE
SEQUENCE_TARGET=950,424
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNAATATCANGCGNTTAANTTTTGTTTNTCNCNATTNNNTTTNTTATNATAATNNNNNNNNNNNNGTATNTAGGTGATATGANAAAAANATAAAAAANGTNAATGANAANAAAGNTGNTTTTAAANTATANTANNNGTTGATTAANAGTGGTTTTACNCANAAATAGAGAANTGAGAGATTATTCGAAGTTTTAANANGTTTGAANAAAATTGTTTGTACATGTTGAAAAANCANANCANGAATTGTTANGATNANAATTGATNGTTCGAACCAAAANTAATGTTANNAAACCATATATGAAACANGGTTTNCCATNATTCATTTATCNNNNNNNNGAANTAGTNNNNNNNTATTTTTCAAACTTCATAAGAAGTNAGTTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCANNTGTTCTTNAATGATACNACNTTGANGTTNTTTTCATNCTGCTTTNTTNAATTCAACTTTGAANGNACATAGTTATCAATTNGAATTAACNTTAAAANGANNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_17027683_17027698_oy-0_ws-0_787_654_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_5_14688546_14689062_tsu-0_wil-2_63963_903881_TRUE
SEQUENCE_TARGET=950,616
SEQUENCE_TEMPLATE=CAAATAATGATAATGANAANAAAGATTAAATANATATTNGAATGNAGTTCAGTNNTGATGAGNTTGCATATATANCTTATAAAAAGTATGGAGNCAATCATGGTTTCAATGTAAGAAAACAACGGAGGATGNAAANAANAGAGAAGGTAGTAAGGCTTCTNTATGTTTGCTCAAAACANGGGTATAGAAAAGAACNTNNTTGTTGTGCTTCTTTGAACACTACGAGAGGGTGTTGGNGGATCAACGNTATAAGGAATTANTTGNTGATTTNAATATGATGCATACCTCTCCGGTATTATGTGCTACTGTAGAGATGTTNNAACATGNAGAGGAANTGTATACACACAAAAGTTTTAGCTTGTTCCAGAAACAATACATAGTTATTGGTGATTATGTTGNCAACAAGGTTAGTAAGTNTGAGATNNTATATAAATATAAAGTATCTTATCGTGGTGTNGCANGTGAGCATTTGGTNAATTATGATGTTGCAAACNAGANAATATGTTGTAGTTGCATGAAGTTTAGTTTTGNTGGGATTTTATGTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCTTTGGATACTCANAAGAAANACATAANTATATACCTAGTGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTTNTGGTTGAGAGAGNNNNNNNNNNNNNNNNNNNNGGACAACTTGNGTCCACTACNATGAATGCNTTGTTCTCNAAANCCCGACCTTTCTTCTTCNCCATGCGAAAAATCAACATTACGGTTTATTAAATCTCTCTTGAAATAAAAAGAAACACAAAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_12744938_12745405_oy-0_wu-0_498051_17827_TRUE
SEQUENCE_TARGET=950,567
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4492892_4493036_can-0_kn-0_37530_20239_TRUE
SEQUENCE_TARGET=950,244
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_5_15591375_15591385_zu-0_rsch-4_108_637154_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=CAAACAAACAAACATATTTTGTGTAACTTGTCAACACTTGCCATTTCATACATCAACTGGATCATTTNNNNNNNNNNNNNNNNNTTCACTTATAGTTTCTTANTGTATTTAGNTAGATTGAAATATGACCANTATNAACATGGTGCGTTGAGGGAACACGNTTTTTTTNNNNNAAAAATCTTAATTATTTCNGACTTGTTATTGATACATTNTAGANTTTTTTGGACCGAGATCTTGGTTGTCCATCAAGGAACACTTTTTGTAGGGTTTGTNACGTCAAACCAAGTCGGAAACCGTCTNTTCACCAAAACGCTAAACATGGGAGATGTTTTTGTGTTTCCAGAGGGATTCATTCACTTCCAATTCAATGTGGNACGATCTCCAGCGGTTGCATTCGCTGCTTTGAGCAGCCAAAACCCCGGTGTCATCTCTATTGTCAACACCGTCTTTGGGTCTAACCCACCAACTAATCTGAATGTTCTTGCTAAGGNATTCCAGTTGGACCCTAGGGTGGCCATGCTCATGGATCTACAAGCCAAAATTCTGATCATGAAATACGAAATAGTGTGTTGCTATTTTANTTCATCCATGCATTTGTTTAAATAAGGACCATGAGTTACCGTTCATTGTAATGCCACGATGTGATAGTTGCCTTTACTTTCAAGTNTCTTCATGAACTTATGTTTCGTTNAAGTGGTGAGTGAANNTATTTACAGATTCAAAATAACATTTTATTAGCATGTTTTGCAGACAATGCATTCATATAACTTTCTATAATANNNNNTTTTNTTTAANAGTTTATNNNAANAAAANGNNNTTAGTCTCTTTNCATACACGAACGNTAGATTNTTACANAACTTTGATCAATTTCGTTNTNTGATAAAATAGCATANTATCAANTTNAATCTNTNTNTTTTTTCACACNTNCNTATTTAGGNGGGNCTNATCTANTGNCGATATANTTTTGNNTNGAATNTNAGTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATANNTTNATNAACATAATTTANAATGANTAATATTGNGCNCNANCGATGNCAACCAATGNTNATTGTTTACNANNNNGAATAAATNAAGATATCAAAATNCATAAAATGACTNAAATACCTTAAAGAGAANGTAATTTGTAANTTAATNATNCAAGATTAAGGAACAAGNCAATNNNNNTATAAATTCATANTGTNAGAATCTTTTAGTTTCTCTNTAAGGNATTTGATGCTGGTAATGTTAGTATTCANGATCTCTNTATCACGTTCAAGTTTGGTACAATCCTTATGTGGTTANTAATAGTACATTAAACCTAAACCAAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_11037064_11037436_ws-0_bur-0_481519_78618_TRUE
SEQUENCE_TARGET=950,472
SEQUENCE_TEMPLATE=GATAAATACCATTTCTCGAATTGTAANCTCAATAACTCTCAATAATAGAGGGTCAAAANCTCTTTTTCTTATGCCAATTGCCTTCAAGCTTTCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATATATATTTAATAGGAGCAATTAATCTCAACTCCAAANTCTCCTACTCTACAGAAATTATTACTANTTTGAGTAGTTTATATGTAATGGTATCAACCCANTCTTATCAACAAAGNATATTTCCCAAGNCAATTTTGAGATTTAAATNAGTTTTTNTTTNNNGNNNNAATTATTATTAANCTGTGGATTTNCAACTTNAAATCNAATNGNGTGGAGAGACTCATATCTTATATATNNNNNNNNNNATNNNACTCAATTTTNNATGTGGGACAATATCTCTAATNCGTCTNNTCGAGATAATGGTTCTTCTGGCCATTCATCTCGATATGTTCAGACATTGATCAGCGGGTCAACTACTGATTGAGCTGATNTGNATTNGGTTGAGCATGTGCAGATCGTGGTATGATANNNNNNNNGANTGTAGGNTTCCAANTNAAAACTAATTGGCAATANATAGAGNANNNNNNNTCTNNTANATACTTTTTAAAATTGTACTCAACTTCNAGTGCAAATNGTACTCAACTTCCATNNAAAGCCNTGCNGGCCACTAACCATAAACNATTTTTTTAGGCCACAATTNCAANANAATTGNGTTANNNNGGNTGGTTTNAGNTGNNNNNNNNNNTNTTTATGCTTCAAATTCAATCNNNTTTANTNACATTTATTCCAATATATAATTTGAACATTTGGTTTTGTATTNTTTTTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNTNNTTTAGTTTTCTNTANTTTATTNTNNANTTNTTNAACTAGACAAATTNGAANATANTTNTTTTTGCCNNAAATCTCAGCANGGCACNGTTCCAATGTGAGANANTATNNNNAATCTANCTATTTTGANGAAAAAANNATTCTTGTNANTACATAATATACTAAGACTACCTTCATTTCAGGTTGACACCGTTCGTGTCGTNTGACCATTTTATCGGCCCTCTCTTCTCGGCGNAAATNCCAAGTNTCAACGTCCAACTTCTCTCTTCTCTCTCACAGACANACTAATATAGAATATGTGTTATATATAGTAATGNAATGATCAATCGAATTTGTATACTAATAGTNATCAAATTATTAAAAAAGTATCAGCTCCACTTAAGATCCAAATCCCTCATCATCAACTCTCCTATATAAANNTTCNAACTCNTGACAATTCATCTCATAAAAAGNTTATNNANNAAAANGGAAAGAAAACCCNAACAATGTCTTNAANTATNTNNNNNNTGGTGGCCATGCAANNNCTCCTTATANGCANNGTTTCTTCCCAAAANGTTACGAATGAGTATTTGAACCNCCAATGCAACAACANACAAGGGACATACACGCGGGGTAGTACGTTTGNGAAGAATCTCAACCAAGTCATCCGTAANATCTCTCACCTCCATCTTCGCTACGGTTACACCTACAACTCCANTGTTGNGGCNTACGAAGTTAGTAANGATCCCAACATTGTCTTCGTTTTGCTCCAGTGNCGTGGTGACTCTTACGGGTCCAAGTGCCANTCCTGCCTCNACACAGCCTTCTCTGGGGTAACTTCACTTCAATATAAGTCACATATTTCCATATATGANCATATACACACGTCTAATCATAGTTAAGNTTGAGAGATAACTATATTAATTTGATTTCATNGATACAACTGATATACATNTTAANGNAAGTAGAAAAGAAAGCTATATGNTATATGTAGATTANTCCATGCANNATNNNNNTNTATATAATTNNATATATATATGNCNAGCTTCGTNAGAGATGTCGGGGAAANAAAGGAGCAATAATATGGTACGACCAATGTGTTTTGGAGATTTCTTCNGTNAATACCAAGGGGAGGATTCGTTACGATAGTTTTTTCAATATGACCAACGTGAAGAATGTGAGCAGCAATGCGGAGNAGTTTAAAAATAAAAGGAAGGATCTCTTCCACAAGCTATTGTTGGGAGCCACCAAGGATGTAAGCGACAGCAATGATGCGTANGCAGTGGGGGAGACNAGGATAGGAAGAAATAAGATGTATGCAATGATGCAGTGNGCACTAGACTTAACGACGAATGGATGCTATGTGTGTCTAGAATGGATTATAGGCAGGTATGATAGCTTCTACTTTGACCGTAGACAAGGANNGAGAGTTTTGAGTAGGANTTGTANCTTAAGATATGAGCTTTACCCTTTTCTTAGGA
PRIMER_LEFT_NUM_RETURNED=12
PRIMER_RIGHT_NUM_RETURNED=12
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=12
PRIMER_PAIR_0_PENALTY=3.660223
PRIMER_LEFT_0_PENALTY=1.555487
PRIMER_RIGHT_0_PENALTY=2.104736
PRIMER_LEFT_0_SEQUENCE=CATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_0_SEQUENCE=ACTACCCCGCGTGTATGTCC
PRIMER_LEFT_0=543,21
PRIMER_RIGHT_0=1528,20
PRIMER_LEFT_0_TM=60.555
PRIMER_RIGHT_0_TM=62.105
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=986
PRIMER_PAIR_1_PENALTY=4.412776
PRIMER_LEFT_1_PENALTY=2.308039
PRIMER_RIGHT_1_PENALTY=2.104736
PRIMER_LEFT_1_SEQUENCE=GAGCATGTGCAGATCGTGGT
PRIMER_RIGHT_1_SEQUENCE=ACTACCCCGCGTGTATGTCC
PRIMER_LEFT_1=540,20
PRIMER_RIGHT_1=1528,20
PRIMER_LEFT_1_TM=62.308
PRIMER_RIGHT_1_TM=62.105
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=989
PRIMER_PAIR_2_PENALTY=4.592815
PRIMER_LEFT_2_PENALTY=1.555487
PRIMER_RIGHT_2_PENALTY=3.037328
PRIMER_LEFT_2_SEQUENCE=CATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_2_SEQUENCE=TACTACCCCGCGTGTATGTCC
PRIMER_LEFT_2=543,21
PRIMER_RIGHT_2=1529,21
PRIMER_LEFT_2_TM=60.555
PRIMER_RIGHT_2_TM=62.037
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=987
PRIMER_PAIR_3_PENALTY=4.634251
PRIMER_LEFT_3_PENALTY=2.529515
PRIMER_RIGHT_3_PENALTY=2.104736
PRIMER_LEFT_3_SEQUENCE=ATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_3_SEQUENCE=ACTACCCCGCGTGTATGTCC
PRIMER_LEFT_3=544,20
PRIMER_RIGHT_3=1528,20
PRIMER_LEFT_3_TM=57.470
PRIMER_RIGHT_3_TM=62.105
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=985
PRIMER_PAIR_4_PENALTY=5.345367
PRIMER_LEFT_4_PENALTY=2.308039
PRIMER_RIGHT_4_PENALTY=3.037328
PRIMER_LEFT_4_SEQUENCE=GAGCATGTGCAGATCGTGGT
PRIMER_RIGHT_4_SEQUENCE=TACTACCCCGCGTGTATGTCC
PRIMER_LEFT_4=540,20
PRIMER_RIGHT_4=1529,21
PRIMER_LEFT_4_TM=62.308
PRIMER_RIGHT_4_TM=62.037
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=990
PRIMER_PAIR_5_PENALTY=5.566843
PRIMER_LEFT_5_PENALTY=2.529515
PRIMER_RIGHT_5_PENALTY=3.037328
PRIMER_LEFT_5_SEQUENCE=ATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_5_SEQUENCE=TACTACCCCGCGTGTATGTCC
PRIMER_LEFT_5=544,20
PRIMER_RIGHT_5=1529,21
PRIMER_LEFT_5_TM=57.470
PRIMER_RIGHT_5_TM=62.037
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=57.143
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=986
PRIMER_PAIR_6_PENALTY=6.289618
PRIMER_LEFT_6_PENALTY=1.555487
PRIMER_RIGHT_6_PENALTY=4.734131
PRIMER_LEFT_6_SEQUENCE=CATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_6_SEQUENCE=TACCCCGCGTGTATGTCCCT
PRIMER_LEFT_6=543,21
PRIMER_RIGHT_6=1526,20
PRIMER_LEFT_6_TM=60.555
PRIMER_RIGHT_6_TM=64.734
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.9000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=984
PRIMER_PAIR_7_PENALTY=7.042170
PRIMER_LEFT_7_PENALTY=2.308039
PRIMER_RIGHT_7_PENALTY=4.734131
PRIMER_LEFT_7_SEQUENCE=GAGCATGTGCAGATCGTGGT
PRIMER_RIGHT_7_SEQUENCE=TACCCCGCGTGTATGTCCCT
PRIMER_LEFT_7=540,20
PRIMER_RIGHT_7=1526,20
PRIMER_LEFT_7_TM=62.308
PRIMER_RIGHT_7_TM=64.734
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=60.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=987
PRIMER_PAIR_8_PENALTY=7.263646
PRIMER_LEFT_8_PENALTY=2.529515
PRIMER_RIGHT_8_PENALTY=4.734131
PRIMER_LEFT_8_SEQUENCE=ATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_8_SEQUENCE=TACCCCGCGTGTATGTCCCT
PRIMER_LEFT_8=544,20
PRIMER_RIGHT_8=1526,20
PRIMER_LEFT_8_TM=57.470
PRIMER_RIGHT_8_TM=64.734
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=60.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=5.9000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=983
PRIMER_PAIR_9_PENALTY=8.378139
PRIMER_LEFT_9_PENALTY=6.273402
PRIMER_RIGHT_9_PENALTY=2.104736
PRIMER_LEFT_9_SEQUENCE=GCATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_9_SEQUENCE=ACTACCCCGCGTGTATGTCC
PRIMER_LEFT_9=542,22
PRIMER_RIGHT_9=1528,20
PRIMER_LEFT_9_TM=64.273
PRIMER_RIGHT_9_TM=62.105
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=60.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=987
PRIMER_PAIR_10_PENALTY=9.310730
PRIMER_LEFT_10_PENALTY=6.273402
PRIMER_RIGHT_10_PENALTY=3.037328
PRIMER_LEFT_10_SEQUENCE=GCATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_10_SEQUENCE=TACTACCCCGCGTGTATGTCC
PRIMER_LEFT_10=542,22
PRIMER_RIGHT_10=1529,21
PRIMER_LEFT_10_TM=64.273
PRIMER_RIGHT_10_TM=62.037
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=57.143
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=988
PRIMER_PAIR_11_PENALTY=11.007533
PRIMER_LEFT_11_PENALTY=6.273402
PRIMER_RIGHT_11_PENALTY=4.734131
PRIMER_LEFT_11_SEQUENCE=GCATGTGCAGATCGTGGTATGA
PRIMER_RIGHT_11_SEQUENCE=TACCCCGCGTGTATGTCCCT
PRIMER_LEFT_11=542,22
PRIMER_RIGHT_11=1526,20
PRIMER_LEFT_11_TM=64.273
PRIMER_RIGHT_11_TM=64.734
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=60.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.9000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=985
=
SEQUENCE_ID=MAGIC.433_1_15081313_15081317_ct-1_wu-0_72504_340583_TRUE
SEQUENCE_TARGET=950,104
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTCTGANANGAGTGAAAAGTAANATCGANGGANTTCNATGTCNGATTGCAAACTAGGANATGAGTCTNATTTANTNGGCNAGGNANTAANGATTGCTAACCGACGAAACNGAGGTTAATTTCAACAAANACTGTTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTNGGTCAAAGAGATGAAGGTTTNGTCGGNTCGNTTTACTTCAGAAAGACTNTNAAAATATTATTTAATNNNNNNNNNNNNNNAAATTANCTATGGTTAANNTACTTTNGANGGGCTTATGGTNAAAAAACTNAAAAAAGTGCCTACTCCCNTCCAAGTGCTNCATTNCATAATNGAAATNCAAAAAAGTACCCTTCTGCCTAATATTCCCCTCTTATAATTAGTCAAATNCATTGGATTGTGAAACATTTTGACCATAAAAACACTAACAAAGCTATTTACTGCTTCTAANGAATTTTTTGTTAGTTTTAGCCTNTTTTGGGAGAAAATGAGTATAAGTGTTGTCTAAACACTCNCAATACATCTCTNACANAGGCGTAAGAATTGTATCCTTGTTAGAATACAGAAAGCCAAAGAATCATATGGATTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGATTGGTTAGTGTTTTGGAGTCAAATATGACTTGATGTCATGTGTATGATTGAGTATAAGAACATAAACCACAACCGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAGACACAAAGCCAAAGACTCATATGGACTTTGGCTACACCATGAAAGCTTGGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGAATATGACTTAATGTCATGTGTATGATTGAGTATAAGAATATAAACCGCAACCGGATCTTAAAGGTGTAAGAATTGTATCCTTGTTAGAAGACACAAAGCCAAAGACTCATATGGACTTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4455310_4455397_bur-0_can-0_87689_37530_TRUE
SEQUENCE_TARGET=950,187
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_12750462_12750779_no-0_wil-2_77222_11382_TRUE
SEQUENCE_TARGET=950,417
SEQUENCE_TEMPLATE=GCAACAAGAGAATCAAGNATTCGGGTTCCTCGGTANCGTGCCCGCATTNTCGAATTCAAAAGNAAGAGAGTGGGAAACCCATGAACTCCATACTTGGAAAGGGTNCTGCAGCANAACAANTAACCAAAAGATCATCACCANAACANANNNNNNNNNNNNNNNNNNNNNACCAGTTTAAANATNNTCACCTAGGCTTAANGGATGATTCCTTAATGGCAAAATGAGGAANGGAGGAATAGAGAGAAGAGATAACATCNAAACTNGGTCTAAATGACCTTGANAATGGACACCATGATGCATAGAAAAGCAAAGCCACATAATCACACTTGTTCNTATGAATCATATCCAATGCTATTTGCAACCAACGCTCATCACCCTNCANCAACATTTGANNACATTCATAAGAAACNAAAGANAGATTCTTNNTCAAGCAGAAACCCTAGAAATTGAAATCNNNNNNNNNNNNTNAATTACCTCGGTGACAGCAACAAAACGAGGACGCTCGTCTGATTCAACACCAGAAACAGAACAAGTCTGATCTCGNNGCCCAAAGATNGAATCTTTANCAGANTTCGTAGCACAAAACGGAACCCTAACGGCGTNGACGTCGGCNACGNTTAAAAACATGATGACCAATAGCAACAACNAGATCTCCTTNTCCATNAAANAANACCCAGAANCTCTCTCTCTCTCTATCTCNNNNNNTCTCCCTACGAAATTCACAGAACCCAGAATAGAAATTNGTTGTTGTTGCCGACAAATTGGAAACAGGTGAGAGGTTGATGTTTCTGGGAATTGAATCAATGNTGTTTCTTTANTGANGAGATNTTTATGCAGCGGTAANGGNNNNNNNNATTTGAGGGAGATATNGANGAAGAAGAANAGGTTGTGGTTGCTGCTGGGNACTGGGTTNTTGCTCGTAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGTCATGACAAAATTAGNNANAAAANAAANTCTAAACCAGNATTTTTANTAAACCGNNNNTTTTNTTTAGTANANTCAGAATTTTTGCTTCAATACGATTTTGATTTCGNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATATTTCAACACGTNGNAAGAAAGANTNTTATTNANNANNAGNNTTTCNNNAATNAAAANTTATATTGNTNNNNNNATACAGTTAATTGTTATTTNANATNGTNTNNNNNTNTNTNTNTTTATTAATATGAGCATTGAAACATATTAAGTTNTCGACACNGTNTATATTTGACCATAATNGNNTTAATANANNNNCATTATAAACACNATTCGGTTNGTTNNTNATGCNNNNNNNNNNAANATCTCTCCCTTTTNCNNNGTTCAAGTNNNCNNNANNACNNTTTNTCNGCAAAGTCNTNATATNCAGNNNTTTTTCCCACNATATCATGNTTTGNTTNNATNTCCNANNNNNNNNNNNANTCTTGTATNCAATTTGTTGATGTGGNGATTGTCNNAGTCCTGAAGTNTACTTANAGTTGGAATCCTNTCAAGGTAGANGNNNANNATCNNGNGNATATNTNTTNCNNTNNACNTTNGNNNTNNTGTNNNNNCATTTTTACCATGTANTAANNNNAAATGTTTATTTNAGAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9335010_9335217_bur-0_po-0_16959_94020_TRUE
SEQUENCE_TARGET=950,307
SEQUENCE_TEMPLATE=AAGCTAAAGGNAGTGCAACTGCAAGCTGTACTTCTATCTAACTACACAAGTTGCANGTGAAGTGTGAACAGAGAAGATTTGNTCTCAATGGCATCTAGTACTTAGATAAGCTCAGGTTTTTAGTTATGATCACCCAAAAGTCTTATCCANGCTATTGATACCTAAGGATCCCCCTACAAGGGANAACGAGTAATCTGAACCGCCTGAGTATAGGCNATAGTCTGAAACTTANNNNNNNNNNNNNNNNNNNNNTTGTTCCCCTAATCACAGTGCTTAATACCTCCTAAAATTTGTTAGCATGACCTATACTGTTTCCACCTCACANGATCACAACACAGANAGGTCACTACTAACGTACAAGTTCATTCAAGACGTGTTTACCTGTTATGTGGATAACTATGATGTCGCAAGAACACATCTGCACCNNNNAAGCTNGAANANGATGATGATGATGATCTCATGCACTGCAANCAGTACNANCCCNTACATTTTAGNGATGACATAGCATCAAAGCAACACAAGAAGGCCCACATATACCAGAGATCCTNTATGGAAGTAAGGGGTCTAAATCTCTGATTAAGTCTAAATNTCATGGCAATTATTCCAGAGCTTATATCAGAATNNTTCATGTATACGTCTCATACNCATAAAGCATGGAAACAATGAGATTGNNNNNNNGTGTTAACTATTGGACANTGGCNGAAATTACATATAATTACTTGACACATNCTTCTTTCATATACAAAATCTNTAATAATATTTGGCATTTGNTNACAGATTTGCCAGCAAAAGAATAGAAAAGAGATATTGCACAGTNTTAGCAAAGAGAAGCAACGCAATGCNGTTAAACAAGTTGGGTTCAGTTACACCTTTGAAAGAAGAGGCCTTATCTTCTCTANCAGTGTCTCNGCTTCAATAAATCCACCCAAAGGTCCCAAAGACTTGTACTTGATGGCTNTCCATCTGTTTTGATGACAATATTGATAACAAAGCTTATNNTNTCACAGNTTTCAAGCCATGGGAAAGTAAGAGAGAGACGCAAANAATTTCNCAAAAAAGATCAACNAGTATGAAGCATATATNTACCTGGGCNCATAAGGCAATGCAAAACAGTGGAGATGGAGATGATCAACACTGTTAAATGGTGGCTGGTGAAAACCAAATCTGTGAGANGAAAGTACAAATCAGGTTAANGCTACAGAAGTCANCAAAAGNANCCTTACTTACTCCAAATTCATAATTGATTTCATTNAGTGTATGTAACCAAGAANNCTCACCTATGAATGCTTTGAGGAGCATCTTTNTGCAACAGTTGTTGTCCCACACTAAGCATGTGTCTTACTGCTCAAATCATNAAACAAACATCCTNATCGAAACTTTACATAGGNNCAANAGAAAANGANGTATAAANATNTTTAGNNAACCAACCAAGTGAGTAGTCTTCATCTCTTCTCTGAAGGTCATTCACAGTAGGAATATGTTCTTTTGGAATCACCAANTAGTGCCTACATCATCAGTGCTTTNTTCNTAAATACNAGTAAATGNTAGCTAANTAAGNAANGANATNCANNNNNNNNNNNNNNNNNNNNNNNAAGNAAACGGAATCAAACTCAATTCANAAAANTNGATANAGCAAAGTGAGTGAACAGAGACACAAACCTCTGGGCTGCNGGNTTGATNTCTTGAAAGGCGATGACTTTCTCATCCTGAAAAANAGATAATCGCAGACGTAAGAAAACTTGTTCAATTTAGCCTCTGATGATGAACCAAACAAACAAGAGAAGGGAAGAAGAAGATAACGGTGTGAAGGAGACGAGTAGTGGTTGGATTTCGCACGATCTCACAGAAGATACACGCCTGATTCACNCCCGCCATAATTCTTACGTTTGGANTTTGAAATCGCTGCTAACCAAAATTCGAAATGGATTTCGATTTCCAAGTTGTGGAAATGGGAAAGTTGTCTTCTCCGGCCAAAAGCCTCTTNTTNTCTCCGAAACTAAATATTTGGATNTTTCATTTTAACCCCACAAATANTTTTANTGCTAAGTTAANCCCTTAANTCATATANGTTCCNATTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.800309
PRIMER_LEFT_0_PENALTY=0.104897
PRIMER_RIGHT_0_PENALTY=0.695412
PRIMER_LEFT_0_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_0_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_0=818,20
PRIMER_RIGHT_0=1667,20
PRIMER_LEFT_0_TM=59.895
PRIMER_RIGHT_0_TM=59.305
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=850
PRIMER_PAIR_1_PENALTY=0.862176
PRIMER_LEFT_1_PENALTY=0.104897
PRIMER_RIGHT_1_PENALTY=0.757279
PRIMER_LEFT_1_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_1_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_1=818,20
PRIMER_RIGHT_1=1707,20
PRIMER_LEFT_1_TM=59.895
PRIMER_RIGHT_1_TM=59.243
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=890
PRIMER_PAIR_2_PENALTY=1.480399
PRIMER_LEFT_2_PENALTY=0.104897
PRIMER_RIGHT_2_PENALTY=1.375502
PRIMER_LEFT_2_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_2_SEQUENCE=CACATGCTTAGTGTGGGACAA
PRIMER_LEFT_2=818,20
PRIMER_RIGHT_2=1335,21
PRIMER_LEFT_2_TM=59.895
PRIMER_RIGHT_2_TM=59.624
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=518
PRIMER_PAIR_3_PENALTY=1.904150
PRIMER_LEFT_3_PENALTY=0.104897
PRIMER_RIGHT_3_PENALTY=1.799253
PRIMER_LEFT_3_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_3_SEQUENCE=TCCCTTCTCTTGTTTGTTTGG
PRIMER_LEFT_3=818,20
PRIMER_RIGHT_3=1786,21
PRIMER_LEFT_3_TM=59.895
PRIMER_RIGHT_3_TM=59.201
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=969
PRIMER_PAIR_4_PENALTY=1.910160
PRIMER_LEFT_4_PENALTY=1.214748
PRIMER_RIGHT_4_PENALTY=0.695412
PRIMER_LEFT_4_SEQUENCE=AGCAAAGAGAAGCAACGCAA
PRIMER_RIGHT_4_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_4=819,20
PRIMER_RIGHT_4=1667,20
PRIMER_LEFT_4_TM=61.215
PRIMER_RIGHT_4_TM=59.305
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.5000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=849
PRIMER_PAIR_5_PENALTY=1.972027
PRIMER_LEFT_5_PENALTY=1.214748
PRIMER_RIGHT_5_PENALTY=0.757279
PRIMER_LEFT_5_SEQUENCE=AGCAAAGAGAAGCAACGCAA
PRIMER_RIGHT_5_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_5=819,20
PRIMER_RIGHT_5=1707,20
PRIMER_LEFT_5_TM=61.215
PRIMER_RIGHT_5_TM=59.243
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=10.5000
PRIMER_RIGHT_5_END_STABILITY=11.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=889
PRIMER_PAIR_6_PENALTY=2.117050
PRIMER_LEFT_6_PENALTY=0.104897
PRIMER_RIGHT_6_PENALTY=2.012153
PRIMER_LEFT_6_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_6_SEQUENCE=TCCCTTCTCTTGTTTGTTTGGT
PRIMER_LEFT_6=818,20
PRIMER_RIGHT_6=1786,22
PRIMER_LEFT_6_TM=59.895
PRIMER_RIGHT_6_TM=60.012
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=8.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=969
PRIMER_PAIR_7_PENALTY=2.214068
PRIMER_LEFT_7_PENALTY=1.674752
PRIMER_RIGHT_7_PENALTY=0.539315
PRIMER_LEFT_7_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_7_SEQUENCE=TTCTGTGAGATCGTGCGAAA
PRIMER_LEFT_7=922,20
PRIMER_RIGHT_7=1847,20
PRIMER_LEFT_7_TM=61.675
PRIMER_RIGHT_7_TM=60.539
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=9.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=926
PRIMER_PAIR_8_PENALTY=2.231263
PRIMER_LEFT_8_PENALTY=1.674752
PRIMER_RIGHT_8_PENALTY=0.556510
PRIMER_LEFT_8_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_8_SEQUENCE=TTTGGTTAGCAGCGATTTCA
PRIMER_LEFT_8=922,20
PRIMER_RIGHT_8=1913,20
PRIMER_LEFT_8_TM=61.675
PRIMER_RIGHT_8_TM=59.443
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=992
PRIMER_PAIR_9_PENALTY=2.352613
PRIMER_LEFT_9_PENALTY=0.104897
PRIMER_RIGHT_9_PENALTY=2.247716
PRIMER_LEFT_9_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_9_SEQUENCE=GCTCCTCAAAGCATTCATAGGT
PRIMER_LEFT_9=818,20
PRIMER_RIGHT_9=1296,22
PRIMER_LEFT_9_TM=59.895
PRIMER_RIGHT_9_TM=59.752
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=479
PRIMER_PAIR_10_PENALTY=2.370165
PRIMER_LEFT_10_PENALTY=1.674752
PRIMER_RIGHT_10_PENALTY=0.695412
PRIMER_LEFT_10_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_10_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_10=922,20
PRIMER_RIGHT_10=1667,20
PRIMER_LEFT_10_TM=61.675
PRIMER_RIGHT_10_TM=59.305
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=746
PRIMER_PAIR_11_PENALTY=2.432031
PRIMER_LEFT_11_PENALTY=1.674752
PRIMER_RIGHT_11_PENALTY=0.757279
PRIMER_LEFT_11_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_11_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_11=922,20
PRIMER_RIGHT_11=1707,20
PRIMER_LEFT_11_TM=61.675
PRIMER_RIGHT_11_TM=59.243
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=786
PRIMER_PAIR_12_PENALTY=2.442911
PRIMER_LEFT_12_PENALTY=0.104897
PRIMER_RIGHT_12_PENALTY=2.338015
PRIMER_LEFT_12_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_12_SEQUENCE=AAGACACATGCTTAGTGTGGGA
PRIMER_LEFT_12=818,20
PRIMER_RIGHT_12=1339,22
PRIMER_LEFT_12_TM=59.895
PRIMER_RIGHT_12_TM=59.662
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.455
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=522
PRIMER_PAIR_13_PENALTY=2.566610
PRIMER_LEFT_13_PENALTY=0.104897
PRIMER_RIGHT_13_PENALTY=2.461713
PRIMER_LEFT_13_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_13_SEQUENCE=CCCTTCTCTTGTTTGTTTGGTT
PRIMER_LEFT_13=818,20
PRIMER_RIGHT_13=1785,22
PRIMER_LEFT_13_TM=59.895
PRIMER_RIGHT_13_TM=59.538
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=8.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=968
PRIMER_PAIR_14_PENALTY=2.579191
PRIMER_LEFT_14_PENALTY=0.104897
PRIMER_RIGHT_14_PENALTY=2.474295
PRIMER_LEFT_14_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_14_SEQUENCE=ACACATGCTTAGTGTGGGACAA
PRIMER_LEFT_14=818,20
PRIMER_RIGHT_14=1336,22
PRIMER_LEFT_14_TM=59.895
PRIMER_RIGHT_14_TM=60.474
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=519
PRIMER_PAIR_15_PENALTY=2.590250
PRIMER_LEFT_15_PENALTY=1.214748
PRIMER_RIGHT_15_PENALTY=1.375502
PRIMER_LEFT_15_SEQUENCE=AGCAAAGAGAAGCAACGCAA
PRIMER_RIGHT_15_SEQUENCE=CACATGCTTAGTGTGGGACAA
PRIMER_LEFT_15=819,20
PRIMER_RIGHT_15=1335,21
PRIMER_LEFT_15_TM=61.215
PRIMER_RIGHT_15_TM=59.624
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=10.5000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=517
PRIMER_PAIR_16_PENALTY=2.590792
PRIMER_LEFT_16_PENALTY=0.104897
PRIMER_RIGHT_16_PENALTY=2.485895
PRIMER_LEFT_16_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_16_SEQUENCE=TTCCCTTCTCTTGTTTGTTTGG
PRIMER_LEFT_16=818,20
PRIMER_RIGHT_16=1787,22
PRIMER_LEFT_16_TM=59.895
PRIMER_RIGHT_16_TM=60.486
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=8.8000
PRIMER_PAIR_16_COMPL_ANY=8.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=970
PRIMER_PAIR_17_PENALTY=2.593830
PRIMER_LEFT_17_PENALTY=2.054514
PRIMER_RIGHT_17_PENALTY=0.539315
PRIMER_LEFT_17_SEQUENCE=TCAATAAATCCACCCAAAGGTC
PRIMER_RIGHT_17_SEQUENCE=TTCTGTGAGATCGTGCGAAA
PRIMER_LEFT_17=912,22
PRIMER_RIGHT_17=1847,20
PRIMER_LEFT_17_TM=60.055
PRIMER_RIGHT_17_TM=60.539
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=9.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=936
PRIMER_PAIR_18_PENALTY=2.653287
PRIMER_LEFT_18_PENALTY=0.104897
PRIMER_RIGHT_18_PENALTY=2.548390
PRIMER_LEFT_18_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_18_SEQUENCE=CGTCTCCTTCACACCGTTATCT
PRIMER_LEFT_18=818,20
PRIMER_RIGHT_18=1814,22
PRIMER_LEFT_18_TM=59.895
PRIMER_RIGHT_18_TM=60.548
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=5.6000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=997
PRIMER_PAIR_19_PENALTY=2.695795
PRIMER_LEFT_19_PENALTY=2.156479
PRIMER_RIGHT_19_PENALTY=0.539315
PRIMER_LEFT_19_SEQUENCE=GCTTCAATAAATCCACCCAAAG
PRIMER_RIGHT_19_SEQUENCE=TTCTGTGAGATCGTGCGAAA
PRIMER_LEFT_19=909,22
PRIMER_RIGHT_19=1847,20
PRIMER_LEFT_19_TM=59.844
PRIMER_RIGHT_19_TM=60.539
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.3000
PRIMER_RIGHT_19_END_STABILITY=9.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=939
PRIMER_PAIR_20_PENALTY=2.708559
PRIMER_LEFT_20_PENALTY=1.674752
PRIMER_RIGHT_20_PENALTY=1.033807
PRIMER_LEFT_20_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_20_SEQUENCE=AACGTAAGAATTATGGCGGG
PRIMER_LEFT_20=922,20
PRIMER_RIGHT_20=1886,20
PRIMER_LEFT_20_TM=61.675
PRIMER_RIGHT_20_TM=58.966
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=12.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=965
PRIMER_PAIR_21_PENALTY=2.711749
PRIMER_LEFT_21_PENALTY=0.104897
PRIMER_RIGHT_21_PENALTY=2.606853
PRIMER_LEFT_21_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_21_SEQUENCE=AAAGCACTGATGATGTAGGCA
PRIMER_LEFT_21=818,20
PRIMER_RIGHT_21=1522,21
PRIMER_LEFT_21_TM=59.895
PRIMER_RIGHT_21_TM=58.393
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=2.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=705
PRIMER_PAIR_22_PENALTY=2.749926
PRIMER_LEFT_22_PENALTY=2.054514
PRIMER_RIGHT_22_PENALTY=0.695412
PRIMER_LEFT_22_SEQUENCE=TCAATAAATCCACCCAAAGGTC
PRIMER_RIGHT_22_SEQUENCE=GCCCAGAGGTTTGTGTCTCT
PRIMER_LEFT_22=912,22
PRIMER_RIGHT_22=1666,20
PRIMER_LEFT_22_TM=60.055
PRIMER_RIGHT_22_TM=59.305
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.6000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=755
PRIMER_PAIR_23_PENALTY=2.749926
PRIMER_LEFT_23_PENALTY=2.054514
PRIMER_RIGHT_23_PENALTY=0.695412
PRIMER_LEFT_23_SEQUENCE=TCAATAAATCCACCCAAAGGTC
PRIMER_RIGHT_23_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_23=912,22
PRIMER_RIGHT_23=1667,20
PRIMER_LEFT_23_TM=60.055
PRIMER_RIGHT_23_TM=59.305
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=6.1000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=756
PRIMER_PAIR_24_PENALTY=2.799815
PRIMER_LEFT_24_PENALTY=1.674752
PRIMER_RIGHT_24_PENALTY=1.125062
PRIMER_LEFT_24_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_24_SEQUENCE=TGCGAAATCCAACCACTACTC
PRIMER_LEFT_24=922,20
PRIMER_RIGHT_24=1834,21
PRIMER_LEFT_24_TM=61.675
PRIMER_RIGHT_24_TM=60.125
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=5.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=913
PRIMER_PAIR_25_PENALTY=2.799842
PRIMER_LEFT_25_PENALTY=0.104897
PRIMER_RIGHT_25_PENALTY=2.694946
PRIMER_LEFT_25_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_25_SEQUENCE=CCCTTCTCTTGTTTGTTTGG
PRIMER_LEFT_25=818,20
PRIMER_RIGHT_25=1785,20
PRIMER_LEFT_25_TM=59.895
PRIMER_RIGHT_25_TM=57.305
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.9000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=8.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=968
PRIMER_PAIR_26_PENALTY=2.811793
PRIMER_LEFT_26_PENALTY=2.054514
PRIMER_RIGHT_26_PENALTY=0.757279
PRIMER_LEFT_26_SEQUENCE=TCAATAAATCCACCCAAAGGTC
PRIMER_RIGHT_26_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_26=912,22
PRIMER_RIGHT_26=1707,20
PRIMER_LEFT_26_TM=60.055
PRIMER_RIGHT_26_TM=59.243
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=11.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=796
PRIMER_PAIR_27_PENALTY=2.828415
PRIMER_LEFT_27_PENALTY=2.289100
PRIMER_RIGHT_27_PENALTY=0.539315
PRIMER_LEFT_27_SEQUENCE=CTTCAATAAATCCACCCAAAGG
PRIMER_RIGHT_27_SEQUENCE=TTCTGTGAGATCGTGCGAAA
PRIMER_LEFT_27=910,22
PRIMER_RIGHT_27=1847,20
PRIMER_LEFT_27_TM=59.711
PRIMER_RIGHT_27_TM=60.539
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=9.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=938
PRIMER_PAIR_28_PENALTY=2.834734
PRIMER_LEFT_28_PENALTY=0.104897
PRIMER_RIGHT_28_PENALTY=2.729838
PRIMER_LEFT_28_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_28_SEQUENCE=AAAGCACTGATGATGTAGGCAC
PRIMER_LEFT_28=818,20
PRIMER_RIGHT_28=1522,22
PRIMER_LEFT_28_TM=59.895
PRIMER_RIGHT_28_TM=59.270
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.9000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=2.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=705
PRIMER_PAIR_29_PENALTY=2.851891
PRIMER_LEFT_29_PENALTY=2.156479
PRIMER_RIGHT_29_PENALTY=0.695412
PRIMER_LEFT_29_SEQUENCE=GCTTCAATAAATCCACCCAAAG
PRIMER_RIGHT_29_SEQUENCE=GCCCAGAGGTTTGTGTCTCT
PRIMER_LEFT_29=909,22
PRIMER_RIGHT_29=1666,20
PRIMER_LEFT_29_TM=59.844
PRIMER_RIGHT_29_TM=59.305
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.3000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=758
PRIMER_PAIR_30_PENALTY=2.851891
PRIMER_LEFT_30_PENALTY=2.156479
PRIMER_RIGHT_30_PENALTY=0.695412
PRIMER_LEFT_30_SEQUENCE=GCTTCAATAAATCCACCCAAAG
PRIMER_RIGHT_30_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_30=909,22
PRIMER_RIGHT_30=1667,20
PRIMER_LEFT_30_TM=59.844
PRIMER_RIGHT_30_TM=59.305
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=6.1000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=759
PRIMER_PAIR_31_PENALTY=2.875509
PRIMER_LEFT_31_PENALTY=0.104897
PRIMER_RIGHT_31_PENALTY=2.770612
PRIMER_LEFT_31_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_31_SEQUENCE=AGACACATGCTTAGTGTGGGA
PRIMER_LEFT_31=818,20
PRIMER_RIGHT_31=1338,21
PRIMER_LEFT_31_TM=59.895
PRIMER_RIGHT_31_TM=58.229
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.9000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=521
PRIMER_PAIR_32_PENALTY=2.875757
PRIMER_LEFT_32_PENALTY=2.180345
PRIMER_RIGHT_32_PENALTY=0.695412
PRIMER_LEFT_32_SEQUENCE=TAGCAAAGAGAAGCAACGCAA
PRIMER_RIGHT_32_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_32=818,21
PRIMER_RIGHT_32=1667,20
PRIMER_LEFT_32_TM=61.180
PRIMER_RIGHT_32_TM=59.305
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=10.5000
PRIMER_RIGHT_32_END_STABILITY=6.1000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=850
PRIMER_PAIR_33_PENALTY=2.875757
PRIMER_LEFT_33_PENALTY=2.180345
PRIMER_RIGHT_33_PENALTY=0.695412
PRIMER_LEFT_33_SEQUENCE=TTAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_33_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_33=817,21
PRIMER_RIGHT_33=1667,20
PRIMER_LEFT_33_TM=61.180
PRIMER_RIGHT_33_TM=59.305
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.9000
PRIMER_RIGHT_33_END_STABILITY=6.1000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=851
PRIMER_PAIR_34_PENALTY=2.881417
PRIMER_LEFT_34_PENALTY=1.674752
PRIMER_RIGHT_34_PENALTY=1.206665
PRIMER_LEFT_34_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_34_SEQUENCE=AAACGTAAGAATTATGGCGGG
PRIMER_LEFT_34=922,20
PRIMER_RIGHT_34=1887,21
PRIMER_LEFT_34_TM=61.675
PRIMER_RIGHT_34_TM=60.207
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=12.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=966
PRIMER_PAIR_35_PENALTY=2.891379
PRIMER_LEFT_35_PENALTY=0.104897
PRIMER_RIGHT_35_PENALTY=2.786482
PRIMER_LEFT_35_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_35_SEQUENCE=CCCTTCTCTTGTTTGTTTGGT
PRIMER_LEFT_35=818,20
PRIMER_RIGHT_35=1785,21
PRIMER_LEFT_35_TM=59.895
PRIMER_RIGHT_35_TM=58.214
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.9000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=8.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=968
PRIMER_PAIR_36_PENALTY=2.913758
PRIMER_LEFT_36_PENALTY=2.156479
PRIMER_RIGHT_36_PENALTY=0.757279
PRIMER_LEFT_36_SEQUENCE=GCTTCAATAAATCCACCCAAAG
PRIMER_RIGHT_36_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_36=909,22
PRIMER_RIGHT_36=1707,20
PRIMER_LEFT_36_TM=59.844
PRIMER_RIGHT_36_TM=59.243
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=11.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=799
PRIMER_PAIR_37_PENALTY=2.937624
PRIMER_LEFT_37_PENALTY=2.180345
PRIMER_RIGHT_37_PENALTY=0.757279
PRIMER_LEFT_37_SEQUENCE=TAGCAAAGAGAAGCAACGCAA
PRIMER_RIGHT_37_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_37=818,21
PRIMER_RIGHT_37=1707,20
PRIMER_LEFT_37_TM=61.180
PRIMER_RIGHT_37_TM=59.243
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.5000
PRIMER_RIGHT_37_END_STABILITY=11.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=890
PRIMER_PAIR_38_PENALTY=2.937624
PRIMER_LEFT_38_PENALTY=2.180345
PRIMER_RIGHT_38_PENALTY=0.757279
PRIMER_LEFT_38_SEQUENCE=TTAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_38_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_38=817,21
PRIMER_RIGHT_38=1707,20
PRIMER_LEFT_38_TM=61.180
PRIMER_RIGHT_38_TM=59.243
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.9000
PRIMER_RIGHT_38_END_STABILITY=11.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=891
PRIMER_PAIR_39_PENALTY=2.984512
PRIMER_LEFT_39_PENALTY=2.289100
PRIMER_RIGHT_39_PENALTY=0.695412
PRIMER_LEFT_39_SEQUENCE=CTTCAATAAATCCACCCAAAGG
PRIMER_RIGHT_39_SEQUENCE=GCCCAGAGGTTTGTGTCTCT
PRIMER_LEFT_39=910,22
PRIMER_RIGHT_39=1666,20
PRIMER_LEFT_39_TM=59.711
PRIMER_RIGHT_39_TM=59.305
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=757
PRIMER_PAIR_40_PENALTY=2.984512
PRIMER_LEFT_40_PENALTY=2.289100
PRIMER_RIGHT_40_PENALTY=0.695412
PRIMER_LEFT_40_SEQUENCE=CTTCAATAAATCCACCCAAAGG
PRIMER_RIGHT_40_SEQUENCE=AGCCCAGAGGTTTGTGTCTC
PRIMER_LEFT_40=910,22
PRIMER_RIGHT_40=1667,20
PRIMER_LEFT_40_TM=59.711
PRIMER_RIGHT_40_TM=59.305
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=6.1000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=758
PRIMER_PAIR_41_PENALTY=2.990898
PRIMER_LEFT_41_PENALTY=2.451582
PRIMER_RIGHT_41_PENALTY=0.539315
PRIMER_LEFT_41_SEQUENCE=GCTTCAATAAATCCACCCAA
PRIMER_RIGHT_41_SEQUENCE=TTCTGTGAGATCGTGCGAAA
PRIMER_LEFT_41=909,20
PRIMER_RIGHT_41=1847,20
PRIMER_LEFT_41_TM=57.548
PRIMER_RIGHT_41_TM=60.539
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=10.0000
PRIMER_RIGHT_41_END_STABILITY=9.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=939
PRIMER_PAIR_42_PENALTY=3.003613
PRIMER_LEFT_42_PENALTY=1.214748
PRIMER_RIGHT_42_PENALTY=1.788865
PRIMER_LEFT_42_SEQUENCE=AGCAAAGAGAAGCAACGCAA
PRIMER_RIGHT_42_SEQUENCE=CCCAGAGGTTTGTGTCTCTGT
PRIMER_LEFT_42=819,20
PRIMER_RIGHT_42=1665,21
PRIMER_LEFT_42_TM=61.215
PRIMER_RIGHT_42_TM=59.211
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.5000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=847
PRIMER_PAIR_43_PENALTY=3.014001
PRIMER_LEFT_43_PENALTY=1.214748
PRIMER_RIGHT_43_PENALTY=1.799253
PRIMER_LEFT_43_SEQUENCE=AGCAAAGAGAAGCAACGCAA
PRIMER_RIGHT_43_SEQUENCE=TCCCTTCTCTTGTTTGTTTGG
PRIMER_LEFT_43=819,20
PRIMER_RIGHT_43=1786,21
PRIMER_LEFT_43_TM=61.215
PRIMER_RIGHT_43_TM=59.201
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.5000
PRIMER_RIGHT_43_END_STABILITY=8.8000
PRIMER_PAIR_43_COMPL_ANY=8.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=968
PRIMER_PAIR_44_PENALTY=3.027531
PRIMER_LEFT_44_PENALTY=2.488216
PRIMER_RIGHT_44_PENALTY=0.539315
PRIMER_LEFT_44_SEQUENCE=TTGGGTTCAGTTACACCTTTGA
PRIMER_RIGHT_44_SEQUENCE=TTCTGTGAGATCGTGCGAAA
PRIMER_LEFT_44=853,22
PRIMER_RIGHT_44=1847,20
PRIMER_LEFT_44_TM=59.512
PRIMER_RIGHT_44_TM=60.539
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=9.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=995
PRIMER_PAIR_45_PENALTY=3.038294
PRIMER_LEFT_45_PENALTY=0.104897
PRIMER_RIGHT_45_PENALTY=2.933397
PRIMER_LEFT_45_SEQUENCE=TAGCAAAGAGAAGCAACGCA
PRIMER_RIGHT_45_SEQUENCE=GACACATGCTTAGTGTGGGA
PRIMER_LEFT_45=818,20
PRIMER_RIGHT_45=1337,20
PRIMER_LEFT_45_TM=59.895
PRIMER_RIGHT_45_TM=57.067
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.9000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=520
PRIMER_PAIR_46_PENALTY=3.046379
PRIMER_LEFT_46_PENALTY=2.289100
PRIMER_RIGHT_46_PENALTY=0.757279
PRIMER_LEFT_46_SEQUENCE=CTTCAATAAATCCACCCAAAGG
PRIMER_RIGHT_46_SEQUENCE=AGGATGAGAAAGTCATCGCC
PRIMER_LEFT_46=910,22
PRIMER_RIGHT_46=1707,20
PRIMER_LEFT_46_TM=59.711
PRIMER_RIGHT_46_TM=59.243
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=11.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=798
PRIMER_PAIR_47_PENALTY=3.050254
PRIMER_LEFT_47_PENALTY=1.674752
PRIMER_RIGHT_47_PENALTY=1.375502
PRIMER_LEFT_47_SEQUENCE=CACCCAAAGGTCCCAAAGAC
PRIMER_RIGHT_47_SEQUENCE=CACATGCTTAGTGTGGGACAA
PRIMER_LEFT_47=922,20
PRIMER_RIGHT_47=1335,21
PRIMER_LEFT_47_TM=61.675
PRIMER_RIGHT_47_TM=59.624
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=414
PRIMER_PAIR_48_PENALTY=3.088321
PRIMER_LEFT_48_PENALTY=2.054514
PRIMER_RIGHT_48_PENALTY=1.033807
PRIMER_LEFT_48_SEQUENCE=TCAATAAATCCACCCAAAGGTC
PRIMER_RIGHT_48_SEQUENCE=AACGTAAGAATTATGGCGGG
PRIMER_LEFT_48=912,22
PRIMER_RIGHT_48=1886,20
PRIMER_LEFT_48_TM=60.055
PRIMER_RIGHT_48_TM=58.966
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=12.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=975
PRIMER_PAIR_49_PENALTY=3.137484
PRIMER_LEFT_49_PENALTY=2.598168
PRIMER_RIGHT_49_PENALTY=0.539315
PRIMER_LEFT_49_SEQUENCE=TCAATAAATCCACCCAAAGG
PRIMER_RIGHT_49_SEQUENCE=TTCTGTGAGATCGTGCGAAA
PRIMER_LEFT_49=912,20
PRIMER_RIGHT_49=1847,20
PRIMER_LEFT_49_TM=57.402
PRIMER_RIGHT_49_TM=60.539
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=9.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=936
=
SEQUENCE_ID=MAGIC.149_4_7009573_7009655_wil-2_edi-0_53179_64622_TRUE
SEQUENCE_TARGET=950,182
SEQUENCE_TEMPLATE=TTCTCTNAACAAGAGTGNGNGGTCAGGACCNTGACGACAAGTCAATTCAAGAAGANTAANNNAATAGAGAACAATTCTCAGTNTCTTGNAATCTTAAAAGAGAAGAACTTCAATNATGTTCCTTTGGACGTAGCCATAGAGATATTTATGAGATNGCCTGTGAAATCCGTAGCTAGGTTTCTCCTACTCTCAAAGTTTTGGGCCGAAATTATCCGAAGTAGACATTTCATCACGTCTTTCCAGGTTAGGTCTTCGTTGCAGCCGCGTCTACTAGTNGTGTTTATTGATTTAAATAAACAATGGAACTGCGANGATTGGTACTTCTTCTNGCTNTCTTCATCAACAANNTCTTNTCTATCACGTGTTACATGTCCCNTTCCAGATTNNGTGCACTATTCTCATTATGTTAATGGCTTGATNAGCCNTNGATNTGGTCTANAGAAANTCATAACTAACCCTAGCACCGGTAAATCCACAGTTTTAGGGAGAGTCCAAACCAATAGTAANGTTGCGNAATGTTTTTTGNGGTATGATCCGGTTAATGATGAATACAAACTATTGGTATTGTGCATGAAGGAAAAAATGCNACGTNATGACGGTCGAACTCGAATATTACAACTATCATCTCAACATCAAGTATTCNCATTGGGAGTTAAAAAGAAACCATGGAGAATGATTGATTATACNACTCCTCACGGTCCTGTNCTNAACNGTGTGTGCATAGATGGTGTTTTATATTATGTTGCTTTTACGGGCGAAGATTTGTCACAGCTNAGCTTNNTGAGATTCGNTTTGGGGTCTGAAAAGTTGGATCTTTTTACTAGTTTACCCGCGGACTTTCCAGCTGCATTTCTACATGGTTTCACTTTGATGAGGTACAAGGGGAAAGTAGNCTTAGCCACTAAAACTTTATTTANTGGTATTGAAGTGTGGNTTTTGGATCANCAAGCCGAAATTCATGGATGNTTGAAGAAAAGTTTCAGTATCAAAGGTGNNNNNANGTGGTTATTTTGTGATTTNTTTATCACAGGCACTACTCATACANGTGAGTTTATTTTGGCACCACGGTTNTATTCTAATAGNTTTTATGTTATCTATTACAATCCCGACACGNAAAGTTTAAGAAAAACCAAGGTTGNAGNANACGGAGGCTATGACTTCAAGCATCGGGAAACAAAAGCAATGGTTTTTNCGGATTANGTAGAGACTGTTNGGTTGTTGTAGGAGANACAGTTTCATGCTTTNGTAACNAAGACTGATCNAAATTNTAANGTAANGGGAAATCTGATATNTAGATCTACGATTATTACATAGTTTGGCCGCTAAAAANCGGATTNTAATTGTACGCGGTCCCAANGNCACACACTNNCGAACTCAGCTTACTACACAGTATTGGTAATACAATACTAATGAGCTTTTATTTGTCTAATTATGCAATCTNGCNTTGCTTAAGCAGTAACTCCCAACAACTTCTTCACATCCTTCTGCAATTGCGAAACAANGATTTGACAAGCAATTAGAAACTTTGTGGCTCTAAACAAAACTCAAGNTTTTGATGATTACATGTGANAGAGACATTACNTCAATGCTGAGAGGNGAGGTAGTTGGTGCGAAACGNTCGACAACATTTCCATCTTTGTCAACCANNAACTTTGCGAAGTTCCACTTNATGCCGTCTCCAAAGAGCCCGCCTTTGCTTGATTTCAGAAACTTGTAGACTGGGGCAGCTTTGTCACCGTTAACATCAACCTNCATTACATAGTGCNAAGNTAGAATCAANTCCAAGTAGAGAACTTATACTCAAAACGAAACATTNTCTNAAAATGNGTCACTGATACTNANACTANATCANNNNNNTCCNAAAATNATTCANTGATGCNNTAANAGCTNNCAACATTCTNNNNNNNNNNNNNNNNNNNNNNCAAGAAAAGTNGTTACCTTGTCGAAAATCGGGTACTCNGCCTTGAAACGAGTACAAGCAAACTGAACAATCTCTTCATTAGTACCAGGCTCTTGATTCCCAAACTGGTTACACGGNAANGCAAGAATCTCGAAACCTAAATNANAAATTGNAACCATCAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.183118
PRIMER_LEFT_0_PENALTY=0.058463
PRIMER_RIGHT_0_PENALTY=0.124655
PRIMER_LEFT_0_SEQUENCE=TTTGGGGTCTGAAAAGTTGG
PRIMER_RIGHT_0_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_0=791,20
PRIMER_RIGHT_0=1730,20
PRIMER_LEFT_0_TM=59.942
PRIMER_RIGHT_0_TM=59.875
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=940
PRIMER_PAIR_1_PENALTY=0.475504
PRIMER_LEFT_1_PENALTY=0.413543
PRIMER_RIGHT_1_PENALTY=0.061961
PRIMER_LEFT_1_SEQUENCE=TTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_1_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_1=746,20
PRIMER_RIGHT_1=1183,20
PRIMER_LEFT_1_TM=59.586
PRIMER_RIGHT_1_TM=59.938
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=438
PRIMER_PAIR_2_PENALTY=0.562159
PRIMER_LEFT_2_PENALTY=0.058463
PRIMER_RIGHT_2_PENALTY=0.503696
PRIMER_LEFT_2_SEQUENCE=TTTGGGGTCTGAAAAGTTGG
PRIMER_RIGHT_2_SEQUENCE=AAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_2=791,20
PRIMER_RIGHT_2=1729,20
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.496
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=939
PRIMER_PAIR_3_PENALTY=0.589169
PRIMER_LEFT_3_PENALTY=0.464514
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=TTGGGGTCTGAAAAGTTGGA
PRIMER_RIGHT_3_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_3=792,20
PRIMER_RIGHT_3=1730,20
PRIMER_LEFT_3_TM=60.465
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=939
PRIMER_PAIR_4_PENALTY=0.917239
PRIMER_LEFT_4_PENALTY=0.413543
PRIMER_RIGHT_4_PENALTY=0.503696
PRIMER_LEFT_4_SEQUENCE=TTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_4_SEQUENCE=AAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_4=746,20
PRIMER_RIGHT_4=1729,20
PRIMER_LEFT_4_TM=59.586
PRIMER_RIGHT_4_TM=59.496
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=984
PRIMER_PAIR_5_PENALTY=0.968210
PRIMER_LEFT_5_PENALTY=0.464514
PRIMER_RIGHT_5_PENALTY=0.503696
PRIMER_LEFT_5_SEQUENCE=TTGGGGTCTGAAAAGTTGGA
PRIMER_RIGHT_5_SEQUENCE=AAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_5=792,20
PRIMER_RIGHT_5=1729,20
PRIMER_LEFT_5_TM=60.465
PRIMER_RIGHT_5_TM=59.496
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=938
PRIMER_PAIR_6_PENALTY=1.021420
PRIMER_LEFT_6_PENALTY=0.413543
PRIMER_RIGHT_6_PENALTY=0.607877
PRIMER_LEFT_6_SEQUENCE=TTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_6_SEQUENCE=CTTTTGTTTCCCGATGCTTG
PRIMER_LEFT_6=746,20
PRIMER_RIGHT_6=1179,20
PRIMER_LEFT_6_TM=59.586
PRIMER_RIGHT_6_TM=60.608
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=434
PRIMER_PAIR_7_PENALTY=1.102648
PRIMER_LEFT_7_PENALTY=0.413543
PRIMER_RIGHT_7_PENALTY=0.689105
PRIMER_LEFT_7_SEQUENCE=TTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_7_SEQUENCE=CGTTTCGCACCAACTACCTC
PRIMER_LEFT_7=746,20
PRIMER_RIGHT_7=1616,20
PRIMER_LEFT_7_TM=59.586
PRIMER_RIGHT_7_TM=60.689
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=871
PRIMER_PAIR_8_PENALTY=1.119247
PRIMER_LEFT_8_PENALTY=1.057286
PRIMER_RIGHT_8_PENALTY=0.061961
PRIMER_LEFT_8_SEQUENCE=GGCCGAAATTATCCGAAGTAG
PRIMER_RIGHT_8_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_8=200,21
PRIMER_RIGHT_8=1183,20
PRIMER_LEFT_8_TM=59.943
PRIMER_RIGHT_8_TM=59.938
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=5.4000
PRIMER_RIGHT_8_END_STABILITY=8.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=984
PRIMER_PAIR_9_PENALTY=1.153619
PRIMER_LEFT_9_PENALTY=0.464514
PRIMER_RIGHT_9_PENALTY=0.689105
PRIMER_LEFT_9_SEQUENCE=TTGGGGTCTGAAAAGTTGGA
PRIMER_RIGHT_9_SEQUENCE=CGTTTCGCACCAACTACCTC
PRIMER_LEFT_9=792,20
PRIMER_RIGHT_9=1616,20
PRIMER_LEFT_9_TM=60.465
PRIMER_RIGHT_9_TM=60.689
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=825
PRIMER_PAIR_10_PENALTY=1.254023
PRIMER_LEFT_10_PENALTY=0.058463
PRIMER_RIGHT_10_PENALTY=1.195560
PRIMER_LEFT_10_SEQUENCE=TTTGGGGTCTGAAAAGTTGG
PRIMER_RIGHT_10_SEQUENCE=TTAACGGTGACAAAGCTGCC
PRIMER_LEFT_10=791,20
PRIMER_RIGHT_10=1741,20
PRIMER_LEFT_10_TM=59.942
PRIMER_RIGHT_10_TM=61.196
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=951
PRIMER_PAIR_11_PENALTY=1.278901
PRIMER_LEFT_11_PENALTY=0.775205
PRIMER_RIGHT_11_PENALTY=0.503696
PRIMER_LEFT_11_SEQUENCE=TAGTTTACCCGCGGACTTTC
PRIMER_RIGHT_11_SEQUENCE=AAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_11=821,20
PRIMER_RIGHT_11=1729,20
PRIMER_LEFT_11_TM=59.225
PRIMER_RIGHT_11_TM=59.496
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=909
PRIMER_PAIR_12_PENALTY=1.280074
PRIMER_LEFT_12_PENALTY=0.207756
PRIMER_RIGHT_12_PENALTY=1.072318
PRIMER_LEFT_12_SEQUENCE=ACCCTAGCACCGGTAAATCC
PRIMER_RIGHT_12_SEQUENCE=AACCATTGCTTTTGTTTCCC
PRIMER_LEFT_12=454,20
PRIMER_RIGHT_12=1187,20
PRIMER_LEFT_12_TM=60.208
PRIMER_RIGHT_12_TM=58.928
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=734
PRIMER_PAIR_13_PENALTY=1.464310
PRIMER_LEFT_13_PENALTY=0.775205
PRIMER_RIGHT_13_PENALTY=0.689105
PRIMER_LEFT_13_SEQUENCE=TAGTTTACCCGCGGACTTTC
PRIMER_RIGHT_13_SEQUENCE=CGTTTCGCACCAACTACCTC
PRIMER_LEFT_13=821,20
PRIMER_RIGHT_13=1616,20
PRIMER_LEFT_13_TM=59.225
PRIMER_RIGHT_13_TM=60.689
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=796
PRIMER_PAIR_14_PENALTY=1.478945
PRIMER_LEFT_14_PENALTY=1.416984
PRIMER_RIGHT_14_PENALTY=0.061961
PRIMER_LEFT_14_SEQUENCE=TTCATCACGTCTTTCCAGGTT
PRIMER_RIGHT_14_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_14=225,21
PRIMER_RIGHT_14=1183,20
PRIMER_LEFT_14_TM=59.583
PRIMER_RIGHT_14_TM=59.938
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=8.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=959
PRIMER_PAIR_15_PENALTY=1.478945
PRIMER_LEFT_15_PENALTY=1.416984
PRIMER_RIGHT_15_PENALTY=0.061961
PRIMER_LEFT_15_SEQUENCE=TTTCATCACGTCTTTCCAGGT
PRIMER_RIGHT_15_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_15=224,21
PRIMER_RIGHT_15=1183,20
PRIMER_LEFT_15_TM=59.583
PRIMER_RIGHT_15_TM=59.938
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=8.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=960
PRIMER_PAIR_16_PENALTY=1.485861
PRIMER_LEFT_16_PENALTY=0.413543
PRIMER_RIGHT_16_PENALTY=1.072318
PRIMER_LEFT_16_SEQUENCE=TTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_16_SEQUENCE=AACCATTGCTTTTGTTTCCC
PRIMER_LEFT_16=746,20
PRIMER_RIGHT_16=1187,20
PRIMER_LEFT_16_TM=59.586
PRIMER_RIGHT_16_TM=58.928
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=442
PRIMER_PAIR_17_PENALTY=1.537742
PRIMER_LEFT_17_PENALTY=1.475781
PRIMER_RIGHT_17_PENALTY=0.061961
PRIMER_LEFT_17_SEQUENCE=CTTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_17_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_17=745,21
PRIMER_RIGHT_17=1183,20
PRIMER_LEFT_17_TM=60.476
PRIMER_RIGHT_17_TM=59.938
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=439
PRIMER_PAIR_18_PENALTY=1.553459
PRIMER_LEFT_18_PENALTY=1.491498
PRIMER_RIGHT_18_PENALTY=0.061961
PRIMER_LEFT_18_SEQUENCE=TATGTTGCTTTTACGGGCGA
PRIMER_RIGHT_18_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_18=738,20
PRIMER_RIGHT_18=1183,20
PRIMER_LEFT_18_TM=61.491
PRIMER_RIGHT_18_TM=59.938
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=11.4000
PRIMER_RIGHT_18_END_STABILITY=8.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=446
PRIMER_PAIR_19_PENALTY=1.616154
PRIMER_LEFT_19_PENALTY=1.491498
PRIMER_RIGHT_19_PENALTY=0.124655
PRIMER_LEFT_19_SEQUENCE=TATGTTGCTTTTACGGGCGA
PRIMER_RIGHT_19_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_19=738,20
PRIMER_RIGHT_19=1730,20
PRIMER_LEFT_19_TM=61.491
PRIMER_RIGHT_19_TM=59.875
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=11.4000
PRIMER_RIGHT_19_END_STABILITY=6.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=993
PRIMER_PAIR_20_PENALTY=1.619356
PRIMER_LEFT_20_PENALTY=0.058463
PRIMER_RIGHT_20_PENALTY=1.560893
PRIMER_LEFT_20_SEQUENCE=TTTGGGGTCTGAAAAGTTGG
PRIMER_RIGHT_20_SEQUENCE=GGATGTGAAGAAGTTGTTGGG
PRIMER_LEFT_20=791,20
PRIMER_RIGHT_20=1481,21
PRIMER_LEFT_20_TM=59.942
PRIMER_RIGHT_20_TM=59.439
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=10.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=691
PRIMER_PAIR_21_PENALTY=1.660073
PRIMER_LEFT_21_PENALTY=0.464514
PRIMER_RIGHT_21_PENALTY=1.195560
PRIMER_LEFT_21_SEQUENCE=TTGGGGTCTGAAAAGTTGGA
PRIMER_RIGHT_21_SEQUENCE=TTAACGGTGACAAAGCTGCC
PRIMER_LEFT_21=792,20
PRIMER_RIGHT_21=1741,20
PRIMER_LEFT_21_TM=60.465
PRIMER_RIGHT_21_TM=61.196
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=950
PRIMER_PAIR_22_PENALTY=1.665164
PRIMER_LEFT_22_PENALTY=1.057286
PRIMER_RIGHT_22_PENALTY=0.607877
PRIMER_LEFT_22_SEQUENCE=GGCCGAAATTATCCGAAGTAG
PRIMER_RIGHT_22_SEQUENCE=CTTTTGTTTCCCGATGCTTG
PRIMER_LEFT_22=200,21
PRIMER_RIGHT_22=1179,20
PRIMER_LEFT_22_TM=59.943
PRIMER_RIGHT_22_TM=60.608
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.4000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=980
PRIMER_PAIR_23_PENALTY=1.709510
PRIMER_LEFT_23_PENALTY=1.584855
PRIMER_RIGHT_23_PENALTY=0.124655
PRIMER_LEFT_23_SEQUENCE=TTGATGAGGTACAAGGGGAAA
PRIMER_RIGHT_23_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_23=867,21
PRIMER_RIGHT_23=1730,20
PRIMER_LEFT_23_TM=59.415
PRIMER_RIGHT_23_TM=59.875
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=6.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=864
PRIMER_PAIR_24_PENALTY=1.709510
PRIMER_LEFT_24_PENALTY=1.584855
PRIMER_RIGHT_24_PENALTY=0.124655
PRIMER_LEFT_24_SEQUENCE=TTTGATGAGGTACAAGGGGAA
PRIMER_RIGHT_24_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_24=866,21
PRIMER_RIGHT_24=1730,20
PRIMER_LEFT_24_TM=59.415
PRIMER_RIGHT_24_TM=59.875
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=6.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=865
PRIMER_PAIR_25_PENALTY=1.722721
PRIMER_LEFT_25_PENALTY=1.598066
PRIMER_RIGHT_25_PENALTY=0.124655
PRIMER_LEFT_25_SEQUENCE=CTAGTTTACCCGCGGACTTT
PRIMER_RIGHT_25_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_25=820,20
PRIMER_RIGHT_25=1730,20
PRIMER_LEFT_25_TM=58.402
PRIMER_RIGHT_25_TM=59.875
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=6.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=911
PRIMER_PAIR_26_PENALTY=1.824817
PRIMER_LEFT_26_PENALTY=1.135712
PRIMER_RIGHT_26_PENALTY=0.689105
PRIMER_LEFT_26_SEQUENCE=CTAGTTTACCCGCGGACTTTC
PRIMER_RIGHT_26_SEQUENCE=CGTTTCGCACCAACTACCTC
PRIMER_LEFT_26=820,21
PRIMER_RIGHT_26=1616,20
PRIMER_LEFT_26_TM=60.136
PRIMER_RIGHT_26_TM=60.689
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=797
PRIMER_PAIR_27_PENALTY=1.837429
PRIMER_LEFT_27_PENALTY=1.775468
PRIMER_RIGHT_27_PENALTY=0.061961
PRIMER_LEFT_27_SEQUENCE=TTCCAGGTTAGGTCTTCGTTG
PRIMER_RIGHT_27_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_27=237,21
PRIMER_RIGHT_27=1183,20
PRIMER_LEFT_27_TM=59.225
PRIMER_RIGHT_27_TM=59.938
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.7000
PRIMER_RIGHT_27_END_STABILITY=8.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=947
PRIMER_PAIR_28_PENALTY=1.855511
PRIMER_LEFT_28_PENALTY=0.058463
PRIMER_RIGHT_28_PENALTY=1.797048
PRIMER_LEFT_28_SEQUENCE=TTTGGGGTCTGAAAAGTTGG
PRIMER_RIGHT_28_SEQUENCE=AAAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_28=791,20
PRIMER_RIGHT_28=1730,21
PRIMER_LEFT_28_TM=59.942
PRIMER_RIGHT_28_TM=60.797
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=940
PRIMER_PAIR_29_PENALTY=1.882304
PRIMER_LEFT_29_PENALTY=1.757649
PRIMER_RIGHT_29_PENALTY=0.124655
PRIMER_LEFT_29_SEQUENCE=GGACTTTCCAGCTGCATTTCT
PRIMER_RIGHT_29_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_29=833,21
PRIMER_RIGHT_29=1730,20
PRIMER_LEFT_29_TM=60.758
PRIMER_RIGHT_29_TM=59.875
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.0000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=898
PRIMER_PAIR_30_PENALTY=1.915789
PRIMER_LEFT_30_PENALTY=1.791133
PRIMER_RIGHT_30_PENALTY=0.124655
PRIMER_LEFT_30_SEQUENCE=ACTAGTTTACCCGCGGACTTT
PRIMER_RIGHT_30_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_30=819,21
PRIMER_RIGHT_30=1730,20
PRIMER_LEFT_30_TM=59.209
PRIMER_RIGHT_30_TM=59.875
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.0000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=912
PRIMER_PAIR_31_PENALTY=1.917370
PRIMER_LEFT_31_PENALTY=0.413543
PRIMER_RIGHT_31_PENALTY=1.503828
PRIMER_LEFT_31_SEQUENCE=TTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_31_SEQUENCE=TTTTGTTTCCCGATGCTTGA
PRIMER_LEFT_31=746,20
PRIMER_RIGHT_31=1178,20
PRIMER_LEFT_31_TM=59.586
PRIMER_RIGHT_31_TM=61.504
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=433
PRIMER_PAIR_32_PENALTY=1.932585
PRIMER_LEFT_32_PENALTY=1.870624
PRIMER_RIGHT_32_PENALTY=0.061961
PRIMER_LEFT_32_SEQUENCE=TCATCACGTCTTTCCAGGTT
PRIMER_RIGHT_32_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_32=226,20
PRIMER_RIGHT_32=1183,20
PRIMER_LEFT_32_TM=58.129
PRIMER_RIGHT_32_TM=59.938
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=8.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=958
PRIMER_PAIR_33_PENALTY=1.932585
PRIMER_LEFT_33_PENALTY=1.870624
PRIMER_RIGHT_33_PENALTY=0.061961
PRIMER_LEFT_33_SEQUENCE=TTCATCACGTCTTTCCAGGT
PRIMER_RIGHT_33_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_33=225,20
PRIMER_RIGHT_33=1183,20
PRIMER_LEFT_33_TM=58.129
PRIMER_RIGHT_33_TM=59.938
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=8.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=959
PRIMER_PAIR_34_PENALTY=1.970765
PRIMER_LEFT_34_PENALTY=0.775205
PRIMER_RIGHT_34_PENALTY=1.195560
PRIMER_LEFT_34_SEQUENCE=TAGTTTACCCGCGGACTTTC
PRIMER_RIGHT_34_SEQUENCE=TTAACGGTGACAAAGCTGCC
PRIMER_LEFT_34=821,20
PRIMER_RIGHT_34=1741,20
PRIMER_LEFT_34_TM=59.225
PRIMER_RIGHT_34_TM=61.196
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=921
PRIMER_PAIR_35_PENALTY=1.974436
PRIMER_LEFT_35_PENALTY=0.413543
PRIMER_RIGHT_35_PENALTY=1.560893
PRIMER_LEFT_35_SEQUENCE=TTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_35_SEQUENCE=GGATGTGAAGAAGTTGTTGGG
PRIMER_LEFT_35=746,20
PRIMER_RIGHT_35=1481,21
PRIMER_LEFT_35_TM=59.586
PRIMER_RIGHT_35_TM=59.439
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=10.0000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=736
PRIMER_PAIR_36_PENALTY=1.985096
PRIMER_LEFT_36_PENALTY=0.207756
PRIMER_RIGHT_36_PENALTY=1.777340
PRIMER_LEFT_36_SEQUENCE=ACCCTAGCACCGGTAAATCC
PRIMER_RIGHT_36_SEQUENCE=GCTTGAAGTCATAGCCTCCGT
PRIMER_LEFT_36=454,20
PRIMER_RIGHT_36=1164,21
PRIMER_LEFT_36_TM=60.208
PRIMER_RIGHT_36_TM=60.777
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=9.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=711
PRIMER_PAIR_37_PENALTY=1.995194
PRIMER_LEFT_37_PENALTY=1.491498
PRIMER_RIGHT_37_PENALTY=0.503696
PRIMER_LEFT_37_SEQUENCE=TATGTTGCTTTTACGGGCGA
PRIMER_RIGHT_37_SEQUENCE=AAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_37=738,20
PRIMER_RIGHT_37=1729,20
PRIMER_LEFT_37_TM=61.491
PRIMER_RIGHT_37_TM=59.496
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=11.4000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=992
PRIMER_PAIR_38_PENALTY=2.024861
PRIMER_LEFT_38_PENALTY=1.416984
PRIMER_RIGHT_38_PENALTY=0.607877
PRIMER_LEFT_38_SEQUENCE=TTCATCACGTCTTTCCAGGTT
PRIMER_RIGHT_38_SEQUENCE=CTTTTGTTTCCCGATGCTTG
PRIMER_LEFT_38=225,21
PRIMER_RIGHT_38=1179,20
PRIMER_LEFT_38_TM=59.583
PRIMER_RIGHT_38_TM=60.608
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=955
PRIMER_PAIR_39_PENALTY=2.024861
PRIMER_LEFT_39_PENALTY=1.416984
PRIMER_RIGHT_39_PENALTY=0.607877
PRIMER_LEFT_39_SEQUENCE=TTTCATCACGTCTTTCCAGGT
PRIMER_RIGHT_39_SEQUENCE=CTTTTGTTTCCCGATGCTTG
PRIMER_LEFT_39=224,21
PRIMER_RIGHT_39=1179,20
PRIMER_LEFT_39_TM=59.583
PRIMER_RIGHT_39_TM=60.608
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=956
PRIMER_PAIR_40_PENALTY=2.025406
PRIMER_LEFT_40_PENALTY=0.464514
PRIMER_RIGHT_40_PENALTY=1.560893
PRIMER_LEFT_40_SEQUENCE=TTGGGGTCTGAAAAGTTGGA
PRIMER_RIGHT_40_SEQUENCE=GGATGTGAAGAAGTTGTTGGG
PRIMER_LEFT_40=792,20
PRIMER_RIGHT_40=1481,21
PRIMER_LEFT_40_TM=60.465
PRIMER_RIGHT_40_TM=59.439
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=10.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=690
PRIMER_PAIR_41_PENALTY=2.083658
PRIMER_LEFT_41_PENALTY=1.475781
PRIMER_RIGHT_41_PENALTY=0.607877
PRIMER_LEFT_41_SEQUENCE=CTTTTACGGGCGAAGATTTGT
PRIMER_RIGHT_41_SEQUENCE=CTTTTGTTTCCCGATGCTTG
PRIMER_LEFT_41=745,21
PRIMER_RIGHT_41=1179,20
PRIMER_LEFT_41_TM=60.476
PRIMER_RIGHT_41_TM=60.608
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=435
PRIMER_PAIR_42_PENALTY=2.084302
PRIMER_LEFT_42_PENALTY=2.022341
PRIMER_RIGHT_42_PENALTY=0.061961
PRIMER_LEFT_42_SEQUENCE=GACATTTCATCACGTCTTTCCA
PRIMER_RIGHT_42_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_42=220,22
PRIMER_RIGHT_42=1183,20
PRIMER_LEFT_42_TM=59.978
PRIMER_RIGHT_42_TM=59.938
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=8.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=964
PRIMER_PAIR_43_PENALTY=2.086311
PRIMER_LEFT_43_PENALTY=1.961655
PRIMER_RIGHT_43_PENALTY=0.124655
PRIMER_LEFT_43_SEQUENCE=TGATGAGGTACAAGGGGAAAG
PRIMER_RIGHT_43_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_43=868,21
PRIMER_RIGHT_43=1730,20
PRIMER_LEFT_43_TM=59.038
PRIMER_RIGHT_43_TM=59.875
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=863
PRIMER_PAIR_44_PENALTY=2.086311
PRIMER_LEFT_44_PENALTY=1.961655
PRIMER_RIGHT_44_PENALTY=0.124655
PRIMER_LEFT_44_SEQUENCE=CTTTGATGAGGTACAAGGGGA
PRIMER_RIGHT_44_SEQUENCE=AAAGCTGCCCCAGTCTACAA
PRIMER_LEFT_44=865,21
PRIMER_RIGHT_44=1730,20
PRIMER_LEFT_44_TM=59.038
PRIMER_RIGHT_44_TM=59.875
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.9000
PRIMER_RIGHT_44_END_STABILITY=6.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=866
PRIMER_PAIR_45_PENALTY=2.088124
PRIMER_LEFT_45_PENALTY=2.026163
PRIMER_RIGHT_45_PENALTY=0.061961
PRIMER_LEFT_45_SEQUENCE=TTTTAGGGAGAGTCCAAACCAA
PRIMER_RIGHT_45_SEQUENCE=ATTGCTTTTGTTTCCCGATG
PRIMER_LEFT_45=478,22
PRIMER_RIGHT_45=1183,20
PRIMER_LEFT_45_TM=59.974
PRIMER_RIGHT_45_TM=59.938
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.6000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=706
PRIMER_PAIR_46_PENALTY=2.088551
PRIMER_LEFT_46_PENALTY=1.584855
PRIMER_RIGHT_46_PENALTY=0.503696
PRIMER_LEFT_46_SEQUENCE=TTGATGAGGTACAAGGGGAAA
PRIMER_RIGHT_46_SEQUENCE=AAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_46=867,21
PRIMER_RIGHT_46=1729,20
PRIMER_LEFT_46_TM=59.415
PRIMER_RIGHT_46_TM=59.496
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=863
PRIMER_PAIR_47_PENALTY=2.088551
PRIMER_LEFT_47_PENALTY=1.584855
PRIMER_RIGHT_47_PENALTY=0.503696
PRIMER_LEFT_47_SEQUENCE=TTTGATGAGGTACAAGGGGAA
PRIMER_RIGHT_47_SEQUENCE=AAGCTGCCCCAGTCTACAAG
PRIMER_LEFT_47=866,21
PRIMER_RIGHT_47=1729,20
PRIMER_LEFT_47_TM=59.415
PRIMER_RIGHT_47_TM=59.496
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=864
PRIMER_PAIR_48_PENALTY=2.099376
PRIMER_LEFT_48_PENALTY=1.491498
PRIMER_RIGHT_48_PENALTY=0.607877
PRIMER_LEFT_48_SEQUENCE=TATGTTGCTTTTACGGGCGA
PRIMER_RIGHT_48_SEQUENCE=CTTTTGTTTCCCGATGCTTG
PRIMER_LEFT_48=738,20
PRIMER_RIGHT_48=1179,20
PRIMER_LEFT_48_TM=61.491
PRIMER_RIGHT_48_TM=60.608
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=11.4000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=442
PRIMER_PAIR_49_PENALTY=2.129605
PRIMER_LEFT_49_PENALTY=1.057286
PRIMER_RIGHT_49_PENALTY=1.072318
PRIMER_LEFT_49_SEQUENCE=GGCCGAAATTATCCGAAGTAG
PRIMER_RIGHT_49_SEQUENCE=AACCATTGCTTTTGTTTCCC
PRIMER_LEFT_49=200,21
PRIMER_RIGHT_49=1187,20
PRIMER_LEFT_49_TM=59.943
PRIMER_RIGHT_49_TM=58.928
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.4000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=988
=
SEQUENCE_ID=MAGIC.175_3_13616606_13616887_sf-2_ws-0_445964_32541_TRUE
SEQUENCE_TARGET=950,381
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_17023878_17024422_zu-0_ws-0_587211_2936_TRUE
SEQUENCE_TARGET=950,644
SEQUENCE_TEMPLATE=CTTCTGNTTTGATTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCNAAACTAGNAAANNTAAAANNGNAACGTANNTAGTGCCTTCCNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_4969500_4969725_wil-2_sf-2_45367_21684_TRUE
SEQUENCE_TARGET=950,325
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14584053_14584290_mt-0_ws-0_32202_134047_TRUE
SEQUENCE_TARGET=950,337
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14830779_14831552_ws-0_mt-0_18361_73361_TRUE
SEQUENCE_TARGET=950,873
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8540020_8540037_wu-0_edi-0_28469_54402_TRUE
SEQUENCE_TARGET=950,117
SEQUENCE_TEMPLATE=AATCTCCTTTGGTATACGATCGAATCGNTCCTATATGATGGTTATGTACGAATTGAATTTNTCTAGATATCGATTTCGAGTTTCTGGNCTCATTTAGAAATGCTAATCGTTTGATATAATCTTCTCGAAGAAATNNNNTTATGTNTTTAGNTTTGTACATCAGATTCTTTCTCTTTTCATTGTTTTTGTTTTGCTTTNTGGAGGTACATGTGTAACNCTTACTTGTTTATGAAATTTTCCAGGGAGAAATCTGCTGTTAGTCACAGTATAATATGGAGTAGACCACTCACTGCAAANCGAGTAGAGATCGATGGAGCTACTGCTTATTCTGTTGATGGTTTGTTTCTATTGTTAATNATATGGTNTTGAATGATATGATATTGTTTGGCTTGTTTGTTACATTTGTTGGCTCTTGTCTTTTATCCTTTGGATAACATATGGTTTTGTATGCTCTGAAGGAACTCCTGCGGATTGCACTGGTTTGGGCTTATCGGAAGCACTNTTNCCTTCTCGTCCTGACCTGGTAAACCATATTATTTGTGTGTCCCTGTAAAAGCCTTCTGATGTAATGTTAGTTTTAAATGATGTGCCNATAGATCTTTCGATTTGTTTTTAATAACTTAAACTTCTGCTTAATTTTATAGGAAACATCTAAGATTTGTTGGTCTNTGTAGTNNTTTTTTTTCCTTCTATTGTCTTTTCTGGAGATTGTGAATTTGTGATGTATGATTNTTTTCTCCATGCAGGTTCTGAGTGGTATAAACGTGGGTAGCAACTGCGGTTATAACATGTCGGTGAACATTTCCTCCNCTGTTTCTCTTTGTTTTGNTTTCCTATTTCCTTACTATGTTATGNTTTTGTACAGATTATTAGGGTGCAACTGTTATTTGNCCGTTTTAAAAATTGCATTTTAAACTTTATAAAACTNCCAAAATTGCTGAAACTTTGTTTAGGATTAGAACAAGATTTTGAGAGTATAGTAAGAGAAACTCTAGTTAGANTGTTTCACTGGAAACCNGATTTCTTCNATGGCCAATTCAAATTGTTATANACCATGTTATGCTTCTATGGTTCCTGCCTGAGTTTATCAAACTAGCACTTACCTGNCTGTTAATTCCANCCCAGCGTCTATTCTGGAACTGTTGCTGGCGCTCGNGAAGCCTTCCTTTATGATGTGCCTTCTGCCTCCATATCATATGATTTTGATTGGTAAGAGCTTAATGTCTTACTTGCAGAGATTTTGCGTCTTACTTTTAAAAANTTTCATAACATGTTTGTTATTTCTTCTATGTTTGTGTTACACTCCTAANTTTTNTTAAGCCTNCATACGGTNTTATTGATACCATCTTCTCTTTTTGCCACTNAATGTCTTCTATTTTATTCACCCTTAATGCTATAGGAAACGTGGAGAGATGAATGCTAATGATTTTGCCCTTTCTGCCCAAGCNTGCTTGCCCATTATCAACGGCATACTCNGTGCAATCAAGAACAAAACTCACCCTNTGCAATGCTTTCTGAATATCGATTTGCCGACTGATATTGCTAACCACAAGGTAAGTCTCGTNGCAGCTTTCAACAGACATTCTCNAATGCCTNCACCTAACNATACCTCTGAGTTTTATATGACTGNCTCTTCTTTCCAAANNAAAAGGGATACAAACTAACTAGACAAGGTAAAAGCATGGGTAAAATGGGATGGAGACAAGTGGAAGAGNAAGCACAAGGGCCGAANATGTTATCAACAATGACAATGGATACAGAGTCAGGAGTANTCTCATCAGANAANGACACANCTNCTCATGCGGGAAAAGATAGCCNTTTATTCAAAAGAGAGGTATATCNTTAANGATTNATCACTATTGTTTCTGCTCTTACTTTCTTTTGNATTCATGAAATTGTGTACCTTCTTNCTCTNTTTGTTGTTAGTTAAGGGCAAGCATCAGTGAAGAAGGGAGTNATTCTCATTACCTAAAAGAAGGNTTTGTAAGCATANCTCCCATANTTGTCTNTTTANNTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.259500
PRIMER_LEFT_0_PENALTY=1.133960
PRIMER_RIGHT_0_PENALTY=0.125540
PRIMER_LEFT_0_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_0_SEQUENCE=TCTCCATCCCATTTTACCCA
PRIMER_LEFT_0=738,20
PRIMER_RIGHT_0=1701,20
PRIMER_LEFT_0_TM=61.134
PRIMER_RIGHT_0_TM=60.126
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=964
PRIMER_PAIR_1_PENALTY=1.266494
PRIMER_LEFT_1_PENALTY=1.140954
PRIMER_RIGHT_1_PENALTY=0.125540
PRIMER_LEFT_1_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_1_SEQUENCE=TCTCCATCCCATTTTACCCA
PRIMER_LEFT_1=734,21
PRIMER_RIGHT_1=1701,20
PRIMER_LEFT_1_TM=59.859
PRIMER_RIGHT_1_TM=60.126
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=968
PRIMER_PAIR_2_PENALTY=1.354805
PRIMER_LEFT_2_PENALTY=1.133960
PRIMER_RIGHT_2_PENALTY=0.220845
PRIMER_LEFT_2_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_2_SEQUENCE=CCACTTGTCTCCATCCCATT
PRIMER_LEFT_2=738,20
PRIMER_RIGHT_2=1708,20
PRIMER_LEFT_2_TM=61.134
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=971
PRIMER_PAIR_3_PENALTY=1.361799
PRIMER_LEFT_3_PENALTY=1.140954
PRIMER_RIGHT_3_PENALTY=0.220845
PRIMER_LEFT_3_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_3_SEQUENCE=CCACTTGTCTCCATCCCATT
PRIMER_LEFT_3=734,21
PRIMER_RIGHT_3=1708,20
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=59.779
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=975
PRIMER_PAIR_4_PENALTY=1.364310
PRIMER_LEFT_4_PENALTY=1.022788
PRIMER_RIGHT_4_PENALTY=0.341522
PRIMER_LEFT_4_SEQUENCE=TTGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_4_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_4=537,21
PRIMER_RIGHT_4=1193,20
PRIMER_LEFT_4_TM=60.023
PRIMER_RIGHT_4_TM=59.658
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=657
PRIMER_PAIR_5_PENALTY=1.373292
PRIMER_LEFT_5_PENALTY=1.031770
PRIMER_RIGHT_5_PENALTY=0.341522
PRIMER_LEFT_5_SEQUENCE=TGCACTGGTTTGGGCTTATC
PRIMER_RIGHT_5_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_5=472,20
PRIMER_RIGHT_5=1193,20
PRIMER_LEFT_5_TM=61.032
PRIMER_RIGHT_5_TM=59.658
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.9000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=722
PRIMER_PAIR_6_PENALTY=1.475482
PRIMER_LEFT_6_PENALTY=1.133960
PRIMER_RIGHT_6_PENALTY=0.341522
PRIMER_LEFT_6_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_6_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_6=738,20
PRIMER_RIGHT_6=1193,20
PRIMER_LEFT_6_TM=61.134
PRIMER_RIGHT_6_TM=59.658
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=456
PRIMER_PAIR_7_PENALTY=1.482476
PRIMER_LEFT_7_PENALTY=1.140954
PRIMER_RIGHT_7_PENALTY=0.341522
PRIMER_LEFT_7_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_7_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_7=734,21
PRIMER_RIGHT_7=1193,20
PRIMER_LEFT_7_TM=59.859
PRIMER_RIGHT_7_TM=59.658
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=460
PRIMER_PAIR_8_PENALTY=1.488841
PRIMER_LEFT_8_PENALTY=1.133960
PRIMER_RIGHT_8_PENALTY=0.354881
PRIMER_LEFT_8_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_8_SEQUENCE=CATCCCATTTTACCCATGCT
PRIMER_LEFT_8=738,20
PRIMER_RIGHT_8=1697,20
PRIMER_LEFT_8_TM=61.134
PRIMER_RIGHT_8_TM=59.645
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=960
PRIMER_PAIR_9_PENALTY=1.495835
PRIMER_LEFT_9_PENALTY=1.140954
PRIMER_RIGHT_9_PENALTY=0.354881
PRIMER_LEFT_9_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_9_SEQUENCE=CATCCCATTTTACCCATGCT
PRIMER_LEFT_9=734,21
PRIMER_RIGHT_9=1697,20
PRIMER_LEFT_9_TM=59.859
PRIMER_RIGHT_9_TM=59.645
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=964
PRIMER_PAIR_10_PENALTY=1.628771
PRIMER_LEFT_10_PENALTY=1.133960
PRIMER_RIGHT_10_PENALTY=0.494811
PRIMER_LEFT_10_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_10_SEQUENCE=CTTCCACTTGTCTCCATCCC
PRIMER_LEFT_10=738,20
PRIMER_RIGHT_10=1711,20
PRIMER_LEFT_10_TM=61.134
PRIMER_RIGHT_10_TM=59.505
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=9.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=974
PRIMER_PAIR_11_PENALTY=1.635765
PRIMER_LEFT_11_PENALTY=1.140954
PRIMER_RIGHT_11_PENALTY=0.494811
PRIMER_LEFT_11_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_11_SEQUENCE=CTTCCACTTGTCTCCATCCC
PRIMER_LEFT_11=734,21
PRIMER_RIGHT_11=1711,20
PRIMER_LEFT_11_TM=59.859
PRIMER_RIGHT_11_TM=59.505
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=9.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=978
PRIMER_PAIR_12_PENALTY=1.647286
PRIMER_LEFT_12_PENALTY=1.022788
PRIMER_RIGHT_12_PENALTY=0.624498
PRIMER_LEFT_12_SEQUENCE=TTGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_12_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_12=537,21
PRIMER_RIGHT_12=1192,20
PRIMER_LEFT_12_TM=60.023
PRIMER_RIGHT_12_TM=60.624
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=656
PRIMER_PAIR_13_PENALTY=1.656268
PRIMER_LEFT_13_PENALTY=1.031770
PRIMER_RIGHT_13_PENALTY=0.624498
PRIMER_LEFT_13_SEQUENCE=TGCACTGGTTTGGGCTTATC
PRIMER_RIGHT_13_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_13=472,20
PRIMER_RIGHT_13=1192,20
PRIMER_LEFT_13_TM=61.032
PRIMER_RIGHT_13_TM=60.624
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=5.9000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=721
PRIMER_PAIR_14_PENALTY=1.710607
PRIMER_LEFT_14_PENALTY=1.585067
PRIMER_RIGHT_14_PENALTY=0.125540
PRIMER_LEFT_14_SEQUENCE=CATGTCGGTGAACATTTCCT
PRIMER_RIGHT_14_SEQUENCE=TCTCCATCCCATTTTACCCA
PRIMER_LEFT_14=787,20
PRIMER_RIGHT_14=1701,20
PRIMER_LEFT_14_TM=58.415
PRIMER_RIGHT_14_TM=60.126
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=915
PRIMER_PAIR_15_PENALTY=1.718640
PRIMER_LEFT_15_PENALTY=1.377118
PRIMER_RIGHT_15_PENALTY=0.341522
PRIMER_LEFT_15_SEQUENCE=TGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_15_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_15=538,20
PRIMER_RIGHT_15=1193,20
PRIMER_LEFT_15_TM=58.623
PRIMER_RIGHT_15_TM=59.658
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=656
PRIMER_PAIR_16_PENALTY=1.746941
PRIMER_LEFT_16_PENALTY=1.621401
PRIMER_RIGHT_16_PENALTY=0.125540
PRIMER_LEFT_16_SEQUENCE=TCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_16_SEQUENCE=TCTCCATCCCATTTTACCCA
PRIMER_LEFT_16=735,20
PRIMER_RIGHT_16=1701,20
PRIMER_LEFT_16_TM=58.379
PRIMER_RIGHT_16_TM=60.126
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=967
PRIMER_PAIR_17_PENALTY=1.758458
PRIMER_LEFT_17_PENALTY=1.133960
PRIMER_RIGHT_17_PENALTY=0.624498
PRIMER_LEFT_17_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_17_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_17=738,20
PRIMER_RIGHT_17=1192,20
PRIMER_LEFT_17_TM=61.134
PRIMER_RIGHT_17_TM=60.624
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=455
PRIMER_PAIR_18_PENALTY=1.765452
PRIMER_LEFT_18_PENALTY=1.140954
PRIMER_RIGHT_18_PENALTY=0.624498
PRIMER_LEFT_18_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_18_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_18=734,21
PRIMER_RIGHT_18=1192,20
PRIMER_LEFT_18_TM=59.859
PRIMER_RIGHT_18_TM=60.624
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=459
PRIMER_PAIR_19_PENALTY=1.802641
PRIMER_LEFT_19_PENALTY=1.677101
PRIMER_RIGHT_19_PENALTY=0.125540
PRIMER_LEFT_19_SEQUENCE=ACATGTCGGTGAACATTTCCT
PRIMER_RIGHT_19_SEQUENCE=TCTCCATCCCATTTTACCCA
PRIMER_LEFT_19=786,21
PRIMER_RIGHT_19=1701,20
PRIMER_LEFT_19_TM=59.323
PRIMER_RIGHT_19_TM=60.126
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=916
PRIMER_PAIR_20_PENALTY=1.805912
PRIMER_LEFT_20_PENALTY=1.585067
PRIMER_RIGHT_20_PENALTY=0.220845
PRIMER_LEFT_20_SEQUENCE=CATGTCGGTGAACATTTCCT
PRIMER_RIGHT_20_SEQUENCE=CCACTTGTCTCCATCCCATT
PRIMER_LEFT_20=787,20
PRIMER_RIGHT_20=1708,20
PRIMER_LEFT_20_TM=58.415
PRIMER_RIGHT_20_TM=59.779
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=922
PRIMER_PAIR_21_PENALTY=1.842246
PRIMER_LEFT_21_PENALTY=1.621401
PRIMER_RIGHT_21_PENALTY=0.220845
PRIMER_LEFT_21_SEQUENCE=TCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_21_SEQUENCE=CCACTTGTCTCCATCCCATT
PRIMER_LEFT_21=735,20
PRIMER_RIGHT_21=1708,20
PRIMER_LEFT_21_TM=58.379
PRIMER_RIGHT_21_TM=59.779
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=974
PRIMER_PAIR_22_PENALTY=1.860646
PRIMER_LEFT_22_PENALTY=1.133960
PRIMER_RIGHT_22_PENALTY=0.726686
PRIMER_LEFT_22_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_22_SEQUENCE=TCCCATTTTACCCATGCTTT
PRIMER_LEFT_22=738,20
PRIMER_RIGHT_22=1695,20
PRIMER_LEFT_22_TM=61.134
PRIMER_RIGHT_22_TM=59.273
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.6000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=958
PRIMER_PAIR_23_PENALTY=1.867640
PRIMER_LEFT_23_PENALTY=1.140954
PRIMER_RIGHT_23_PENALTY=0.726686
PRIMER_LEFT_23_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_23_SEQUENCE=TCCCATTTTACCCATGCTTT
PRIMER_LEFT_23=734,21
PRIMER_RIGHT_23=1695,20
PRIMER_LEFT_23_TM=59.859
PRIMER_RIGHT_23_TM=59.273
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=962
PRIMER_PAIR_24_PENALTY=1.897946
PRIMER_LEFT_24_PENALTY=1.677101
PRIMER_RIGHT_24_PENALTY=0.220845
PRIMER_LEFT_24_SEQUENCE=ACATGTCGGTGAACATTTCCT
PRIMER_RIGHT_24_SEQUENCE=CCACTTGTCTCCATCCCATT
PRIMER_LEFT_24=786,21
PRIMER_RIGHT_24=1708,20
PRIMER_LEFT_24_TM=59.323
PRIMER_RIGHT_24_TM=59.779
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=923
PRIMER_PAIR_25_PENALTY=1.926589
PRIMER_LEFT_25_PENALTY=1.585067
PRIMER_RIGHT_25_PENALTY=0.341522
PRIMER_LEFT_25_SEQUENCE=CATGTCGGTGAACATTTCCT
PRIMER_RIGHT_25_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_25=787,20
PRIMER_RIGHT_25=1193,20
PRIMER_LEFT_25_TM=58.415
PRIMER_RIGHT_25_TM=59.658
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=407
PRIMER_PAIR_26_PENALTY=1.939948
PRIMER_LEFT_26_PENALTY=1.585067
PRIMER_RIGHT_26_PENALTY=0.354881
PRIMER_LEFT_26_SEQUENCE=CATGTCGGTGAACATTTCCT
PRIMER_RIGHT_26_SEQUENCE=CATCCCATTTTACCCATGCT
PRIMER_LEFT_26=787,20
PRIMER_RIGHT_26=1697,20
PRIMER_LEFT_26_TM=58.415
PRIMER_RIGHT_26_TM=59.645
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=911
PRIMER_PAIR_27_PENALTY=1.962923
PRIMER_LEFT_27_PENALTY=1.621401
PRIMER_RIGHT_27_PENALTY=0.341522
PRIMER_LEFT_27_SEQUENCE=TCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_27_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_27=735,20
PRIMER_RIGHT_27=1193,20
PRIMER_LEFT_27_TM=58.379
PRIMER_RIGHT_27_TM=59.658
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=459
PRIMER_PAIR_28_PENALTY=1.976282
PRIMER_LEFT_28_PENALTY=1.621401
PRIMER_RIGHT_28_PENALTY=0.354881
PRIMER_LEFT_28_SEQUENCE=TCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_28_SEQUENCE=CATCCCATTTTACCCATGCT
PRIMER_LEFT_28=735,20
PRIMER_RIGHT_28=1697,20
PRIMER_LEFT_28_TM=58.379
PRIMER_RIGHT_28_TM=59.645
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=963
PRIMER_PAIR_29_PENALTY=2.001616
PRIMER_LEFT_29_PENALTY=1.377118
PRIMER_RIGHT_29_PENALTY=0.624498
PRIMER_LEFT_29_SEQUENCE=TGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_29_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_29=538,20
PRIMER_RIGHT_29=1192,20
PRIMER_LEFT_29_TM=58.623
PRIMER_RIGHT_29_TM=60.624
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=655
PRIMER_PAIR_30_PENALTY=2.018623
PRIMER_LEFT_30_PENALTY=1.677101
PRIMER_RIGHT_30_PENALTY=0.341522
PRIMER_LEFT_30_SEQUENCE=ACATGTCGGTGAACATTTCCT
PRIMER_RIGHT_30_SEQUENCE=GATATGGAGGCAGAAGGCAC
PRIMER_LEFT_30=786,21
PRIMER_RIGHT_30=1193,20
PRIMER_LEFT_30_TM=59.323
PRIMER_RIGHT_30_TM=59.658
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=408
PRIMER_PAIR_31_PENALTY=2.031982
PRIMER_LEFT_31_PENALTY=1.677101
PRIMER_RIGHT_31_PENALTY=0.354881
PRIMER_LEFT_31_SEQUENCE=ACATGTCGGTGAACATTTCCT
PRIMER_RIGHT_31_SEQUENCE=CATCCCATTTTACCCATGCT
PRIMER_LEFT_31=786,21
PRIMER_RIGHT_31=1697,20
PRIMER_LEFT_31_TM=59.323
PRIMER_RIGHT_31_TM=59.645
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=912
PRIMER_PAIR_32_PENALTY=2.079878
PRIMER_LEFT_32_PENALTY=1.585067
PRIMER_RIGHT_32_PENALTY=0.494811
PRIMER_LEFT_32_SEQUENCE=CATGTCGGTGAACATTTCCT
PRIMER_RIGHT_32_SEQUENCE=CTTCCACTTGTCTCCATCCC
PRIMER_LEFT_32=787,20
PRIMER_RIGHT_32=1711,20
PRIMER_LEFT_32_TM=58.415
PRIMER_RIGHT_32_TM=59.505
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.3000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=925
PRIMER_PAIR_33_PENALTY=2.116212
PRIMER_LEFT_33_PENALTY=1.621401
PRIMER_RIGHT_33_PENALTY=0.494811
PRIMER_LEFT_33_SEQUENCE=TCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_33_SEQUENCE=CTTCCACTTGTCTCCATCCC
PRIMER_LEFT_33=735,20
PRIMER_RIGHT_33=1711,20
PRIMER_LEFT_33_TM=58.379
PRIMER_RIGHT_33_TM=59.505
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=9.3000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=977
PRIMER_PAIR_34_PENALTY=2.171912
PRIMER_LEFT_34_PENALTY=1.677101
PRIMER_RIGHT_34_PENALTY=0.494811
PRIMER_LEFT_34_SEQUENCE=ACATGTCGGTGAACATTTCCT
PRIMER_RIGHT_34_SEQUENCE=CTTCCACTTGTCTCCATCCC
PRIMER_LEFT_34=786,21
PRIMER_RIGHT_34=1711,20
PRIMER_LEFT_34_TM=59.323
PRIMER_RIGHT_34_TM=59.505
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=9.3000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=926
PRIMER_PAIR_35_PENALTY=2.209565
PRIMER_LEFT_35_PENALTY=1.585067
PRIMER_RIGHT_35_PENALTY=0.624498
PRIMER_LEFT_35_SEQUENCE=CATGTCGGTGAACATTTCCT
PRIMER_RIGHT_35_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_35=787,20
PRIMER_RIGHT_35=1192,20
PRIMER_LEFT_35_TM=58.415
PRIMER_RIGHT_35_TM=60.624
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=406
PRIMER_PAIR_36_PENALTY=2.243838
PRIMER_LEFT_36_PENALTY=1.133960
PRIMER_RIGHT_36_PENALTY=1.109878
PRIMER_LEFT_36_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_36_SEQUENCE=GGTTAGCAATATCAGTCGGCA
PRIMER_LEFT_36=738,20
PRIMER_RIGHT_36=1547,21
PRIMER_LEFT_36_TM=61.134
PRIMER_RIGHT_36_TM=60.110
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=11.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=810
PRIMER_PAIR_37_PENALTY=2.245899
PRIMER_LEFT_37_PENALTY=1.621401
PRIMER_RIGHT_37_PENALTY=0.624498
PRIMER_LEFT_37_SEQUENCE=TCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_37_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_37=735,20
PRIMER_RIGHT_37=1192,20
PRIMER_LEFT_37_TM=58.379
PRIMER_RIGHT_37_TM=60.624
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=458
PRIMER_PAIR_38_PENALTY=2.250832
PRIMER_LEFT_38_PENALTY=1.140954
PRIMER_RIGHT_38_PENALTY=1.109878
PRIMER_LEFT_38_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_38_SEQUENCE=GGTTAGCAATATCAGTCGGCA
PRIMER_LEFT_38=734,21
PRIMER_RIGHT_38=1547,21
PRIMER_LEFT_38_TM=59.859
PRIMER_RIGHT_38_TM=60.110
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=11.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=814
PRIMER_PAIR_39_PENALTY=2.299417
PRIMER_LEFT_39_PENALTY=1.022788
PRIMER_RIGHT_39_PENALTY=1.276629
PRIMER_LEFT_39_SEQUENCE=TTGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_39_SEQUENCE=GGCAGAAGGCACATCATAAA
PRIMER_LEFT_39=537,21
PRIMER_RIGHT_39=1185,20
PRIMER_LEFT_39_TM=60.023
PRIMER_RIGHT_39_TM=58.723
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=6.2000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=649
PRIMER_PAIR_40_PENALTY=2.301076
PRIMER_LEFT_40_PENALTY=1.022788
PRIMER_RIGHT_40_PENALTY=1.278288
PRIMER_LEFT_40_SEQUENCE=TTGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_40_SEQUENCE=GCAGAAGGCACATCATAAAGG
PRIMER_LEFT_40=537,21
PRIMER_RIGHT_40=1184,21
PRIMER_LEFT_40_TM=60.023
PRIMER_RIGHT_40_TM=59.722
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=648
PRIMER_PAIR_41_PENALTY=2.301076
PRIMER_LEFT_41_PENALTY=1.022788
PRIMER_RIGHT_41_PENALTY=1.278288
PRIMER_LEFT_41_SEQUENCE=TTGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_41_SEQUENCE=AGGCAGAAGGCACATCATAAA
PRIMER_LEFT_41=537,21
PRIMER_RIGHT_41=1186,21
PRIMER_LEFT_41_TM=60.023
PRIMER_RIGHT_41_TM=59.722
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=6.2000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=650
PRIMER_PAIR_42_PENALTY=2.301599
PRIMER_LEFT_42_PENALTY=1.677101
PRIMER_RIGHT_42_PENALTY=0.624498
PRIMER_LEFT_42_SEQUENCE=ACATGTCGGTGAACATTTCCT
PRIMER_RIGHT_42_SEQUENCE=ATATGGAGGCAGAAGGCACA
PRIMER_LEFT_42=786,21
PRIMER_RIGHT_42=1192,20
PRIMER_LEFT_42_TM=59.323
PRIMER_RIGHT_42_TM=60.624
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=407
PRIMER_PAIR_43_PENALTY=2.308399
PRIMER_LEFT_43_PENALTY=1.031770
PRIMER_RIGHT_43_PENALTY=1.276629
PRIMER_LEFT_43_SEQUENCE=TGCACTGGTTTGGGCTTATC
PRIMER_RIGHT_43_SEQUENCE=GGCAGAAGGCACATCATAAA
PRIMER_LEFT_43=472,20
PRIMER_RIGHT_43=1185,20
PRIMER_LEFT_43_TM=61.032
PRIMER_RIGHT_43_TM=58.723
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=5.9000
PRIMER_RIGHT_43_END_STABILITY=6.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=714
PRIMER_PAIR_44_PENALTY=2.310058
PRIMER_LEFT_44_PENALTY=1.031770
PRIMER_RIGHT_44_PENALTY=1.278288
PRIMER_LEFT_44_SEQUENCE=TGCACTGGTTTGGGCTTATC
PRIMER_RIGHT_44_SEQUENCE=GCAGAAGGCACATCATAAAGG
PRIMER_LEFT_44=472,20
PRIMER_RIGHT_44=1184,21
PRIMER_LEFT_44_TM=61.032
PRIMER_RIGHT_44_TM=59.722
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=5.9000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=713
PRIMER_PAIR_45_PENALTY=2.310058
PRIMER_LEFT_45_PENALTY=1.031770
PRIMER_RIGHT_45_PENALTY=1.278288
PRIMER_LEFT_45_SEQUENCE=TGCACTGGTTTGGGCTTATC
PRIMER_RIGHT_45_SEQUENCE=AGGCAGAAGGCACATCATAAA
PRIMER_LEFT_45=472,20
PRIMER_RIGHT_45=1186,21
PRIMER_LEFT_45_TM=61.032
PRIMER_RIGHT_45_TM=59.722
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=5.9000
PRIMER_RIGHT_45_END_STABILITY=6.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=715
PRIMER_PAIR_46_PENALTY=2.311753
PRIMER_LEFT_46_PENALTY=1.585067
PRIMER_RIGHT_46_PENALTY=0.726686
PRIMER_LEFT_46_SEQUENCE=CATGTCGGTGAACATTTCCT
PRIMER_RIGHT_46_SEQUENCE=TCCCATTTTACCCATGCTTT
PRIMER_LEFT_46=787,20
PRIMER_RIGHT_46=1695,20
PRIMER_LEFT_46_TM=58.415
PRIMER_RIGHT_46_TM=59.273
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=909
PRIMER_PAIR_47_PENALTY=2.327213
PRIMER_LEFT_47_PENALTY=1.133960
PRIMER_RIGHT_47_PENALTY=1.193253
PRIMER_LEFT_47_SEQUENCE=CCATGCAGGTTCTGAGTGGT
PRIMER_RIGHT_47_SEQUENCE=CCCATTTTACCCATGCTTTT
PRIMER_LEFT_47=738,20
PRIMER_RIGHT_47=1694,20
PRIMER_LEFT_47_TM=61.134
PRIMER_RIGHT_47_TM=58.807
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=957
PRIMER_PAIR_48_PENALTY=2.331804
PRIMER_LEFT_48_PENALTY=1.022788
PRIMER_RIGHT_48_PENALTY=1.309015
PRIMER_LEFT_48_SEQUENCE=TTGTGTGTCCCTGTAAAAGCC
PRIMER_RIGHT_48_SEQUENCE=GCATTCATCTCTCCACGTTTC
PRIMER_LEFT_48=537,21
PRIMER_RIGHT_48=1419,21
PRIMER_LEFT_48_TM=60.023
PRIMER_RIGHT_48_TM=59.691
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=883
PRIMER_PAIR_49_PENALTY=2.334207
PRIMER_LEFT_49_PENALTY=1.140954
PRIMER_RIGHT_49_PENALTY=1.193253
PRIMER_LEFT_49_SEQUENCE=TTCTCCATGCAGGTTCTGAGT
PRIMER_RIGHT_49_SEQUENCE=CCCATTTTACCCATGCTTTT
PRIMER_LEFT_49=734,21
PRIMER_RIGHT_49=1694,20
PRIMER_LEFT_49_TM=59.859
PRIMER_RIGHT_49_TM=58.807
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=961
=
SEQUENCE_ID=MAGIC.338_2_6271658_6271998_edi-0_sf-2_15829_14911_TRUE
SEQUENCE_TARGET=950,440
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTATATTATANCAGAACAAATTGAGTTCACAAAAGANAAAGAGTAAANAAGAAANATNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCGACATTGCNNNATATCTCANGCNNCGCATTTGTNAAGTAATCATTGTAAATGAAANNCANTAAGNTCTCTGCTNCTTTNTNACNANGATTTTTNCGTTGAGTTNAAANAGAATTCAANATGTCGAANTNNNGTAAACNTTTTACCATTGTNCATGTCTTGAATGTGGCATCAGAATATTCNATCTTGNTATTAGTTCCAAATNGAAGATTTTATCGTTGATAACNCANGAAGTGTCATACATTNTTTCACCCATAGCANNNNNNNNNTTTCTCTCAAGTATTCCCNTCTTTGGGTCAAANANNGTGCAATGAGTGNCATNGTTGAGGATGAAAANNATCNTTCAAATAAAAANCAAAGNTTNGCAAAGCTAATCNGTCNAANGTNTTTTGAATNATTATCTCCNAATTTNATCCAAAGATTNCTNTTTTTTATACCANNNNNNNNNNNNNNNNNNNNNNNNCTCTTTCAACTGNAGTNTTAACTNNGTAATCTCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTAATTCTGATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTNNNNNNNNNCTNCAATGTTNGACCNACCANATTTTTTGTGNNNTACAAAGNAATAACCAATCTTAGTNCACTTCAANCNACCTTNTTGCGGANNNNGTAAATNTTCATGAATCTCATTCGTATNNNNTTCGTGGNGTTCNTAACGGCACCGTNTTANNCNGNATTGAGTANNNNNNTTCTTCTAATAGATTTAATTGGGCTAATGGATCGGCCCACTTCGNCCNACGGGNATGAATATATATGATCCTANTAAAGCCAGNAAGGACACNNAGCCAANTTTNNNNNAATCTAAAGATGGGGAAANCGAAGCGATACTGGTTACTNAAAACNGAGCCAANTGAGTGGTCATGGTCAGACCAAGAATCNAACGGCGGNATCAGCAAATGGGACGGCGTCAAGAACAAACAAGCCCAGAANAATCTTAAANCCATGACTTTAGGAGATCTCTGTTTCTTCTACCATTCAGGNACCAAANCNAGATNCGTCGTTGGNGTGGTTGAAGTATCACNTGAATGGTACACTGNTGATGNTGAAGGAGTAGAAGGAGAAGGAGCGGTTGATGTNAAAGCGATTGGAGAAATGAGGAAATGTGTGGATTTGAAGGAAATGAAAGGAGACAAAGGGATTANTACTAANGGTTTTGTTTTGTTTAGACAGCCGAGGTTGTCTGTTGTNCCTGTTGANGAAGATGTTTGGAATANGATTTGTGAATTGGGAAATGGGTTTTGTGGAGATGGTAAGGAAGATTGTGAAAGTAGTGATGNNNNATGANNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=7
PRIMER_RIGHT_NUM_RETURNED=7
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=7
PRIMER_PAIR_0_PENALTY=3.537562
PRIMER_LEFT_0_PENALTY=1.813616
PRIMER_RIGHT_0_PENALTY=1.723946
PRIMER_LEFT_0_SEQUENCE=TGTCTTGAATGTGGCATCAG
PRIMER_RIGHT_0_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_0=852,20
PRIMER_RIGHT_0=1772,20
PRIMER_LEFT_0_TM=58.186
PRIMER_RIGHT_0_TM=61.724
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=5.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=921
PRIMER_PAIR_1_PENALTY=4.012350
PRIMER_LEFT_1_PENALTY=2.288404
PRIMER_RIGHT_1_PENALTY=1.723946
PRIMER_LEFT_1_SEQUENCE=GTCTTGAATGTGGCATCAGAA
PRIMER_RIGHT_1_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_1=853,21
PRIMER_RIGHT_1=1772,20
PRIMER_LEFT_1_TM=58.712
PRIMER_RIGHT_1_TM=61.724
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=920
PRIMER_PAIR_2_PENALTY=4.139334
PRIMER_LEFT_2_PENALTY=2.415388
PRIMER_RIGHT_2_PENALTY=1.723946
PRIMER_LEFT_2_SEQUENCE=ATGTCTTGAATGTGGCATCAG
PRIMER_RIGHT_2_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_2=851,21
PRIMER_RIGHT_2=1772,20
PRIMER_LEFT_2_TM=58.585
PRIMER_RIGHT_2_TM=61.724
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=922
PRIMER_PAIR_3_PENALTY=4.285401
PRIMER_LEFT_3_PENALTY=2.561455
PRIMER_RIGHT_3_PENALTY=1.723946
PRIMER_LEFT_3_SEQUENCE=CATGTCTTGAATGTGGCATC
PRIMER_RIGHT_3_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_3=850,20
PRIMER_RIGHT_3=1772,20
PRIMER_LEFT_3_TM=57.439
PRIMER_RIGHT_3_TM=61.724
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=923
PRIMER_PAIR_4_PENALTY=5.217554
PRIMER_LEFT_4_PENALTY=3.493609
PRIMER_RIGHT_4_PENALTY=1.723946
PRIMER_LEFT_4_SEQUENCE=CATGTCTTGAATGTGGCATCAG
PRIMER_RIGHT_4_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_4=850,22
PRIMER_RIGHT_4=1772,20
PRIMER_LEFT_4_TM=61.494
PRIMER_RIGHT_4_TM=61.724
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=923
PRIMER_PAIR_5_PENALTY=5.335947
PRIMER_LEFT_5_PENALTY=3.612001
PRIMER_RIGHT_5_PENALTY=1.723946
PRIMER_LEFT_5_SEQUENCE=TGTCTTGAATGTGGCATCAGAA
PRIMER_RIGHT_5_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_5=852,22
PRIMER_RIGHT_5=1772,20
PRIMER_LEFT_5_TM=61.612
PRIMER_RIGHT_5_TM=61.724
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=5.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=921
PRIMER_PAIR_6_PENALTY=10.104730
PRIMER_LEFT_6_PENALTY=8.380785
PRIMER_RIGHT_6_PENALTY=1.723946
PRIMER_LEFT_6_SEQUENCE=CATGTCTTGAATGTGGCATCAGAA
PRIMER_RIGHT_6_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_6=850,24
PRIMER_RIGHT_6=1772,20
PRIMER_LEFT_6_TM=64.381
PRIMER_RIGHT_6_TM=61.724
PRIMER_LEFT_6_GC_PERCENT=41.667
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=5.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.287_3_14470751_14471181_no-0_sf-2_15412_7695_TRUE
SEQUENCE_TARGET=950,530
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTGCAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCATNGGTGTTGTNTAGATGAGAACCTATCAACACANNAGGATNAAGNACTTGGTATATTATAATAAAATTATNTGTGNTTTNNNNGAAGAGAATTATGGTCNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_11087237_11087247_zu-0_sf-2_42383_9546_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNATAATTAATTCAANGACTANAAANGGATACATTTAAGGTAGAATAATTGTGTGTAAATGAGGATTAAATGTATTCTTTGGGTATAAATTATGGGTCAAANACTATGTAAAGACACACNCATTTTTGTGCAAATTNGNTATACACATTTTCTNTACANAACTCANAGTTTTCTCTATAATCATCTATNTATTTTCTAGTATATTTGTCNTCAAGAATAATCCTTCTCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGATTCTTGAGTCGTCAGATTTCTTCTGTGTATTACATANCCGAAGTAAGCCGCAATNTNTGNAGAGGATGCTACCAACGCTTCGATAGCCGAAACCCTCAAGACCATGCAACAAACTATGGGATATGGNGCAGAAGTTTNNAAACGTGGAAAAAANCGTCGAAACACTGCAAACAACCCAGGTATCGCTTAGCCAAAATGTTAGTACCATTCNGTCTCATGTCCGTTCNTTAAATGCNGGAAATTCTACTAGAGTTCGAAGGACTATCTTCGGATCCCCAAACTCCCTTCCACGAAATGCAACAAGCGATCAAACGACCCTGGATGGTACAAACGCGAATAGTCAACCGGAATATGGAGAAACNCATCCTAAAGATGATNACCAGTTCGATGATCAGCACGAGCAAGAAAACTTNNATCAGCTNGAAACCATGAGAGAANTAAGTCGGGAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_4328046_4328288_wil-2_ws-0_11541_30629_TRUE
SEQUENCE_TARGET=950,342
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13149578_13149620_ler-0_tsu-0_9533_1453_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_24037382_24037396_bur-0_sf-2_20720_11721_TRUE
SEQUENCE_TARGET=950,114
SEQUENCE_TEMPLATE=GGTTCCNTGANNNNNNNNNNNNNNANATATCTTAGCAAGTCAGGATCNGNNCTACGGTAAGTTAACTNNNNNNGATCATTGTTTGTTCTTTCTGCTCTGTTTCAATACAAGGGTTGTTTTCATTGACACTTAGAAAAGGTCATACATAAGGTTTTAAGGCAATCAATATGATCATTTTTNTNTGNNTTNNTNCTAAAACAACATTGNTNCCNNCCCCCAGNTACANGAAAGATNTNTATGATCGCACATGGNNNNNNNNTNGAGCCAGTTTTCGGACGGGATGGACCCAGANNNNNNNNNNNNNNGAAGTGAACAATTCCAACAATTATGCTCCACNNNNNNNNNCGCNNNNAAATGNNGNCANACNGANTAATGCCAGTGCACCATTGACGATTNAATGGCCTNNNNNNNNNNNNNNTCAGGAAGTCCCAGGGANCAATATTACTTNNNNNNACACTTTGCTGANATCCANGACTTGCNGNCNAATGATANCAGNGAATTCAACANCCTANNNNNCNGNGAAGTNTTNTCTGACCCTATCATTCCTAAGANANTTGACATAACTAGTGTCCAAAGTGNNNCNNCANAAACTTGCNAAGNAGGGAANNGNANTTNGCANCTAACAAGAACCAACCGATCAACTCTTCCACCTCTACTTAACNNNNNNNNNNNNNNNNNNGTNATCCAGTTNCCGCANTCNGAAACAANNGANANTGACGGNATGNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTAGAATNAACTGGCAAGGNGATCCATGTGTCCCTNAANNNNNNNNNNNNNNNNNNNNNNNTTGCAGNAANACGGATNNCTCCACACCACCAAGAATNACTTCTTTGNAANTNGTNNNNTNNNNNNNNNNNNNCANTNNCTCTTCAANANCNGTTNGTANTCATGATCNTTANNNNNNNNTTTGAATGTGGTTTTGAAAANNTNACCAGAAACTTGTCTTCNAGTGGTTTAANTGGAANCATAGCNGCTGCNATTCAAAANCTNACGCAACTAGAAAAACTGTAAGTATNNCNNNNNNNNNNNTTGNAAGCANGAAATTGTTCTATANATAATANCATANNAATTCTCTCTTNCTAACATNAGGGACTTGTCGAATAANAACTTGACNGGAGNNGTGCCGGAGTTTCTAGGCAACATNNAATCNTTGTNNNTCATGTAANTNNNTCTCNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAACAGAAANTTAAGNGGGAACAATCTTANTGGNTCAATNCCTCAANCTCTTCNNAANANGNGACTAGANCTATNGTAAGAANCTTTCTACNTAGNTTTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANANCCTTGNCTTNTTGTAGTGTTGAAGGAAACCCAAGGCTNTGTCTCTCTGATTCATGNAGNAAACNNCCTAAGNAGANANTTCNTNTGGCAATTGTTGCATCNGTTGNTTCTGCNGCNNTTGTCNTTGCTGTANTGATTNTTTTTCTTNTACTCAGAAAGANAAAGTCAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATCTTGTNAATGNNNNNNNNCCNTTGCATNAAANGAAGANANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTGNNGANGTNACNTTTGCTAATAAGAANAGNANAAGGTTCACNTATTNAGAGGTCNTCNAAATGACAAANNACTTNCAAAGAGNNTTAGGTAAAGGAGGGTTNGGCANGGTGTATCANGGTACTGTAAANGGTTCTGANCANGTGGCCGTTAANGTANNNTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.820920
PRIMER_LEFT_0_PENALTY=0.345214
PRIMER_RIGHT_0_PENALTY=0.475706
PRIMER_LEFT_0_SEQUENCE=CCGATCAACTCTTCCACCTC
PRIMER_RIGHT_0_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_0=633,20
PRIMER_RIGHT_0=1554,20
PRIMER_LEFT_0_TM=59.655
PRIMER_RIGHT_0_TM=60.476
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=922
PRIMER_PAIR_1_PENALTY=0.920569
PRIMER_LEFT_1_PENALTY=0.345214
PRIMER_RIGHT_1_PENALTY=0.575355
PRIMER_LEFT_1_SEQUENCE=CCGATCAACTCTTCCACCTC
PRIMER_RIGHT_1_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_1=633,20
PRIMER_RIGHT_1=1552,20
PRIMER_LEFT_1_TM=59.655
PRIMER_RIGHT_1_TM=59.425
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=920
PRIMER_PAIR_2_PENALTY=1.483592
PRIMER_LEFT_2_PENALTY=1.007886
PRIMER_RIGHT_2_PENALTY=0.475706
PRIMER_LEFT_2_SEQUENCE=AACCGATCAACTCTTCCACC
PRIMER_RIGHT_2_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_2=631,20
PRIMER_RIGHT_2=1554,20
PRIMER_LEFT_2_TM=58.992
PRIMER_RIGHT_2_TM=60.476
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=924
PRIMER_PAIR_3_PENALTY=1.483592
PRIMER_LEFT_3_PENALTY=1.007886
PRIMER_RIGHT_3_PENALTY=0.475706
PRIMER_LEFT_3_SEQUENCE=ACCAACCGATCAACTCTTCC
PRIMER_RIGHT_3_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_3=628,20
PRIMER_RIGHT_3=1554,20
PRIMER_LEFT_3_TM=58.992
PRIMER_RIGHT_3_TM=60.476
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=927
PRIMER_PAIR_4_PENALTY=1.488843
PRIMER_LEFT_4_PENALTY=1.013137
PRIMER_RIGHT_4_PENALTY=0.475706
PRIMER_LEFT_4_SEQUENCE=AACCGATCAACTCTTCCACCT
PRIMER_RIGHT_4_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_4=631,21
PRIMER_RIGHT_4=1554,20
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.476
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=924
PRIMER_PAIR_5_PENALTY=1.510112
PRIMER_LEFT_5_PENALTY=1.034406
PRIMER_RIGHT_5_PENALTY=0.475706
PRIMER_LEFT_5_SEQUENCE=CCAACCGATCAACTCTTCCA
PRIMER_RIGHT_5_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_5=629,20
PRIMER_RIGHT_5=1554,20
PRIMER_LEFT_5_TM=61.034
PRIMER_RIGHT_5_TM=60.476
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=926
PRIMER_PAIR_6_PENALTY=1.583242
PRIMER_LEFT_6_PENALTY=1.007886
PRIMER_RIGHT_6_PENALTY=0.575355
PRIMER_LEFT_6_SEQUENCE=AACCGATCAACTCTTCCACC
PRIMER_RIGHT_6_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_6=631,20
PRIMER_RIGHT_6=1552,20
PRIMER_LEFT_6_TM=58.992
PRIMER_RIGHT_6_TM=59.425
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=922
PRIMER_PAIR_7_PENALTY=1.583242
PRIMER_LEFT_7_PENALTY=1.007886
PRIMER_RIGHT_7_PENALTY=0.575355
PRIMER_LEFT_7_SEQUENCE=ACCAACCGATCAACTCTTCC
PRIMER_RIGHT_7_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_7=628,20
PRIMER_RIGHT_7=1552,20
PRIMER_LEFT_7_TM=58.992
PRIMER_RIGHT_7_TM=59.425
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=925
PRIMER_PAIR_8_PENALTY=1.588492
PRIMER_LEFT_8_PENALTY=1.013137
PRIMER_RIGHT_8_PENALTY=0.575355
PRIMER_LEFT_8_SEQUENCE=AACCGATCAACTCTTCCACCT
PRIMER_RIGHT_8_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_8=631,21
PRIMER_RIGHT_8=1552,20
PRIMER_LEFT_8_TM=59.987
PRIMER_RIGHT_8_TM=59.425
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=922
PRIMER_PAIR_9_PENALTY=1.609761
PRIMER_LEFT_9_PENALTY=1.034406
PRIMER_RIGHT_9_PENALTY=0.575355
PRIMER_LEFT_9_SEQUENCE=CCAACCGATCAACTCTTCCA
PRIMER_RIGHT_9_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_9=629,20
PRIMER_RIGHT_9=1552,20
PRIMER_LEFT_9_TM=61.034
PRIMER_RIGHT_9_TM=59.425
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=924
PRIMER_PAIR_10_PENALTY=1.725455
PRIMER_LEFT_10_PENALTY=0.345214
PRIMER_RIGHT_10_PENALTY=1.380241
PRIMER_LEFT_10_SEQUENCE=CCGATCAACTCTTCCACCTC
PRIMER_RIGHT_10_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_10=633,20
PRIMER_RIGHT_10=1552,21
PRIMER_LEFT_10_TM=59.655
PRIMER_RIGHT_10_TM=60.380
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=920
PRIMER_PAIR_11_PENALTY=1.830739
PRIMER_LEFT_11_PENALTY=1.355034
PRIMER_RIGHT_11_PENALTY=0.475706
PRIMER_LEFT_11_SEQUENCE=AACCAACCGATCAACTCTTCC
PRIMER_RIGHT_11_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_11=627,21
PRIMER_RIGHT_11=1554,20
PRIMER_LEFT_11_TM=60.355
PRIMER_RIGHT_11_TM=60.476
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=928
PRIMER_PAIR_12_PENALTY=1.883143
PRIMER_LEFT_12_PENALTY=1.407437
PRIMER_RIGHT_12_PENALTY=0.475706
PRIMER_LEFT_12_SEQUENCE=ACCGATCAACTCTTCCACCT
PRIMER_RIGHT_12_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_12=632,20
PRIMER_RIGHT_12=1554,20
PRIMER_LEFT_12_TM=58.593
PRIMER_RIGHT_12_TM=60.476
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=923
PRIMER_PAIR_13_PENALTY=1.892690
PRIMER_LEFT_13_PENALTY=1.416984
PRIMER_RIGHT_13_PENALTY=0.475706
PRIMER_LEFT_13_SEQUENCE=CAAGAACCAACCGATCAACTC
PRIMER_RIGHT_13_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_13=623,21
PRIMER_RIGHT_13=1554,20
PRIMER_LEFT_13_TM=59.583
PRIMER_RIGHT_13_TM=60.476
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=932
PRIMER_PAIR_14_PENALTY=1.930389
PRIMER_LEFT_14_PENALTY=1.355034
PRIMER_RIGHT_14_PENALTY=0.575355
PRIMER_LEFT_14_SEQUENCE=AACCAACCGATCAACTCTTCC
PRIMER_RIGHT_14_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_14=627,21
PRIMER_RIGHT_14=1552,20
PRIMER_LEFT_14_TM=60.355
PRIMER_RIGHT_14_TM=59.425
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=926
PRIMER_PAIR_15_PENALTY=1.980257
PRIMER_LEFT_15_PENALTY=1.504551
PRIMER_RIGHT_15_PENALTY=0.475706
PRIMER_LEFT_15_SEQUENCE=ACCGATCAACTCTTCCACCTC
PRIMER_RIGHT_15_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_15=632,21
PRIMER_RIGHT_15=1554,20
PRIMER_LEFT_15_TM=60.505
PRIMER_RIGHT_15_TM=60.476
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=923
PRIMER_PAIR_16_PENALTY=1.982792
PRIMER_LEFT_16_PENALTY=1.407437
PRIMER_RIGHT_16_PENALTY=0.575355
PRIMER_LEFT_16_SEQUENCE=ACCGATCAACTCTTCCACCT
PRIMER_RIGHT_16_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_16=632,20
PRIMER_RIGHT_16=1552,20
PRIMER_LEFT_16_TM=58.593
PRIMER_RIGHT_16_TM=59.425
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=921
PRIMER_PAIR_17_PENALTY=1.992339
PRIMER_LEFT_17_PENALTY=1.416984
PRIMER_RIGHT_17_PENALTY=0.575355
PRIMER_LEFT_17_SEQUENCE=CAAGAACCAACCGATCAACTC
PRIMER_RIGHT_17_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_17=623,21
PRIMER_RIGHT_17=1552,20
PRIMER_LEFT_17_TM=59.583
PRIMER_RIGHT_17_TM=59.425
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=930
PRIMER_PAIR_18_PENALTY=2.079906
PRIMER_LEFT_18_PENALTY=1.504551
PRIMER_RIGHT_18_PENALTY=0.575355
PRIMER_LEFT_18_SEQUENCE=ACCGATCAACTCTTCCACCTC
PRIMER_RIGHT_18_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_18=632,21
PRIMER_RIGHT_18=1552,20
PRIMER_LEFT_18_TM=60.505
PRIMER_RIGHT_18_TM=59.425
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=921
PRIMER_PAIR_19_PENALTY=2.109655
PRIMER_LEFT_19_PENALTY=1.633949
PRIMER_RIGHT_19_PENALTY=0.475706
PRIMER_LEFT_19_SEQUENCE=CCGATCAACTCTTCCACCTCT
PRIMER_RIGHT_19_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_19=633,21
PRIMER_RIGHT_19=1554,20
PRIMER_LEFT_19_TM=60.634
PRIMER_RIGHT_19_TM=60.476
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=922
PRIMER_PAIR_20_PENALTY=2.209304
PRIMER_LEFT_20_PENALTY=1.633949
PRIMER_RIGHT_20_PENALTY=0.575355
PRIMER_LEFT_20_SEQUENCE=CCGATCAACTCTTCCACCTCT
PRIMER_RIGHT_20_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_20=633,21
PRIMER_RIGHT_20=1552,20
PRIMER_LEFT_20_TM=60.634
PRIMER_RIGHT_20_TM=59.425
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=920
PRIMER_PAIR_21_PENALTY=2.346330
PRIMER_LEFT_21_PENALTY=1.870624
PRIMER_RIGHT_21_PENALTY=0.475706
PRIMER_LEFT_21_SEQUENCE=CAACCGATCAACTCTTCCAC
PRIMER_RIGHT_21_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_21=630,20
PRIMER_RIGHT_21=1554,20
PRIMER_LEFT_21_TM=58.129
PRIMER_RIGHT_21_TM=60.476
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=925
PRIMER_PAIR_22_PENALTY=2.388127
PRIMER_LEFT_22_PENALTY=1.007886
PRIMER_RIGHT_22_PENALTY=1.380241
PRIMER_LEFT_22_SEQUENCE=AACCGATCAACTCTTCCACC
PRIMER_RIGHT_22_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_22=631,20
PRIMER_RIGHT_22=1552,21
PRIMER_LEFT_22_TM=58.992
PRIMER_RIGHT_22_TM=60.380
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=922
PRIMER_PAIR_23_PENALTY=2.388127
PRIMER_LEFT_23_PENALTY=1.007886
PRIMER_RIGHT_23_PENALTY=1.380241
PRIMER_LEFT_23_SEQUENCE=ACCAACCGATCAACTCTTCC
PRIMER_RIGHT_23_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_23=628,20
PRIMER_RIGHT_23=1552,21
PRIMER_LEFT_23_TM=58.992
PRIMER_RIGHT_23_TM=60.380
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.1000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=925
PRIMER_PAIR_24_PENALTY=2.393378
PRIMER_LEFT_24_PENALTY=1.013137
PRIMER_RIGHT_24_PENALTY=1.380241
PRIMER_LEFT_24_SEQUENCE=AACCGATCAACTCTTCCACCT
PRIMER_RIGHT_24_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_24=631,21
PRIMER_RIGHT_24=1552,21
PRIMER_LEFT_24_TM=59.987
PRIMER_RIGHT_24_TM=60.380
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=922
PRIMER_PAIR_25_PENALTY=2.414647
PRIMER_LEFT_25_PENALTY=1.034406
PRIMER_RIGHT_25_PENALTY=1.380241
PRIMER_LEFT_25_SEQUENCE=CCAACCGATCAACTCTTCCA
PRIMER_RIGHT_25_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_25=629,20
PRIMER_RIGHT_25=1552,21
PRIMER_LEFT_25_TM=61.034
PRIMER_RIGHT_25_TM=60.380
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.1000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=924
PRIMER_PAIR_26_PENALTY=2.427526
PRIMER_LEFT_26_PENALTY=1.951820
PRIMER_RIGHT_26_PENALTY=0.475706
PRIMER_LEFT_26_SEQUENCE=AACAAGAACCAACCGATCAA
PRIMER_RIGHT_26_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_26=621,20
PRIMER_RIGHT_26=1554,20
PRIMER_LEFT_26_TM=58.048
PRIMER_RIGHT_26_TM=60.476
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=934
PRIMER_PAIR_27_PENALTY=2.445979
PRIMER_LEFT_27_PENALTY=1.870624
PRIMER_RIGHT_27_PENALTY=0.575355
PRIMER_LEFT_27_SEQUENCE=CAACCGATCAACTCTTCCAC
PRIMER_RIGHT_27_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_27=630,20
PRIMER_RIGHT_27=1552,20
PRIMER_LEFT_27_TM=58.129
PRIMER_RIGHT_27_TM=59.425
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=923
PRIMER_PAIR_28_PENALTY=2.527175
PRIMER_LEFT_28_PENALTY=1.951820
PRIMER_RIGHT_28_PENALTY=0.575355
PRIMER_LEFT_28_SEQUENCE=AACAAGAACCAACCGATCAA
PRIMER_RIGHT_28_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_28=621,20
PRIMER_RIGHT_28=1552,20
PRIMER_LEFT_28_TM=58.048
PRIMER_RIGHT_28_TM=59.425
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=932
PRIMER_PAIR_29_PENALTY=2.528231
PRIMER_LEFT_29_PENALTY=2.052525
PRIMER_RIGHT_29_PENALTY=0.475706
PRIMER_LEFT_29_SEQUENCE=AACAAGAACCAACCGATCAAC
PRIMER_RIGHT_29_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_29=621,21
PRIMER_RIGHT_29=1554,20
PRIMER_LEFT_29_TM=58.947
PRIMER_RIGHT_29_TM=60.476
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=934
PRIMER_PAIR_30_PENALTY=2.568732
PRIMER_LEFT_30_PENALTY=2.093026
PRIMER_RIGHT_30_PENALTY=0.475706
PRIMER_LEFT_30_SEQUENCE=AACAAGAACCAACCGATCAACT
PRIMER_RIGHT_30_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_30=621,22
PRIMER_RIGHT_30=1554,20
PRIMER_LEFT_30_TM=59.907
PRIMER_RIGHT_30_TM=60.476
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=934
PRIMER_PAIR_31_PENALTY=2.627880
PRIMER_LEFT_31_PENALTY=2.052525
PRIMER_RIGHT_31_PENALTY=0.575355
PRIMER_LEFT_31_SEQUENCE=AACAAGAACCAACCGATCAAC
PRIMER_RIGHT_31_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_31=621,21
PRIMER_RIGHT_31=1552,20
PRIMER_LEFT_31_TM=58.947
PRIMER_RIGHT_31_TM=59.425
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=932
PRIMER_PAIR_32_PENALTY=2.645209
PRIMER_LEFT_32_PENALTY=0.345214
PRIMER_RIGHT_32_PENALTY=2.299995
PRIMER_LEFT_32_SEQUENCE=CCGATCAACTCTTCCACCTC
PRIMER_RIGHT_32_SEQUENCE=GCCTTGGGTTTCCTTCAACA
PRIMER_LEFT_32=633,20
PRIMER_RIGHT_32=1553,20
PRIMER_LEFT_32_TM=59.655
PRIMER_RIGHT_32_TM=62.300
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=921
PRIMER_PAIR_33_PENALTY=2.668381
PRIMER_LEFT_33_PENALTY=2.093026
PRIMER_RIGHT_33_PENALTY=0.575355
PRIMER_LEFT_33_SEQUENCE=AACAAGAACCAACCGATCAACT
PRIMER_RIGHT_33_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_33=621,22
PRIMER_RIGHT_33=1552,20
PRIMER_LEFT_33_TM=59.907
PRIMER_RIGHT_33_TM=59.425
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=932
PRIMER_PAIR_34_PENALTY=2.735275
PRIMER_LEFT_34_PENALTY=1.355034
PRIMER_RIGHT_34_PENALTY=1.380241
PRIMER_LEFT_34_SEQUENCE=AACCAACCGATCAACTCTTCC
PRIMER_RIGHT_34_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_34=627,21
PRIMER_RIGHT_34=1552,21
PRIMER_LEFT_34_TM=60.355
PRIMER_RIGHT_34_TM=60.380
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.1000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=926
PRIMER_PAIR_35_PENALTY=2.787678
PRIMER_LEFT_35_PENALTY=1.407437
PRIMER_RIGHT_35_PENALTY=1.380241
PRIMER_LEFT_35_SEQUENCE=ACCGATCAACTCTTCCACCT
PRIMER_RIGHT_35_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_35=632,20
PRIMER_RIGHT_35=1552,21
PRIMER_LEFT_35_TM=58.593
PRIMER_RIGHT_35_TM=60.380
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=921
PRIMER_PAIR_36_PENALTY=2.797225
PRIMER_LEFT_36_PENALTY=1.416984
PRIMER_RIGHT_36_PENALTY=1.380241
PRIMER_LEFT_36_SEQUENCE=CAAGAACCAACCGATCAACTC
PRIMER_RIGHT_36_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_36=623,21
PRIMER_RIGHT_36=1552,21
PRIMER_LEFT_36_TM=59.583
PRIMER_RIGHT_36_TM=60.380
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=6.1000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=930
PRIMER_PAIR_37_PENALTY=2.833429
PRIMER_LEFT_37_PENALTY=0.345214
PRIMER_RIGHT_37_PENALTY=2.488216
PRIMER_LEFT_37_SEQUENCE=CCGATCAACTCTTCCACCTC
PRIMER_RIGHT_37_SEQUENCE=TGGGTTTCCTTCAACACTACAA
PRIMER_LEFT_37=633,20
PRIMER_RIGHT_37=1549,22
PRIMER_LEFT_37_TM=59.655
PRIMER_RIGHT_37_TM=59.512
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=6.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=917
PRIMER_PAIR_38_PENALTY=2.833429
PRIMER_LEFT_38_PENALTY=0.345214
PRIMER_RIGHT_38_PENALTY=2.488216
PRIMER_LEFT_38_SEQUENCE=CCGATCAACTCTTCCACCTC
PRIMER_RIGHT_38_SEQUENCE=TTGGGTTTCCTTCAACACTACA
PRIMER_LEFT_38=633,20
PRIMER_RIGHT_38=1550,22
PRIMER_LEFT_38_TM=59.655
PRIMER_RIGHT_38_TM=59.512
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=5.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=918
PRIMER_PAIR_39_PENALTY=2.834259
PRIMER_LEFT_39_PENALTY=2.358553
PRIMER_RIGHT_39_PENALTY=0.475706
PRIMER_LEFT_39_SEQUENCE=CAAGAACCAACCGATCAACT
PRIMER_RIGHT_39_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_39=623,20
PRIMER_RIGHT_39=1554,20
PRIMER_LEFT_39_TM=57.641
PRIMER_RIGHT_39_TM=60.476
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=932
PRIMER_PAIR_40_PENALTY=2.841937
PRIMER_LEFT_40_PENALTY=2.366231
PRIMER_RIGHT_40_PENALTY=0.475706
PRIMER_LEFT_40_SEQUENCE=GAACCAACCGATCAACTCTTC
PRIMER_RIGHT_40_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_40=626,21
PRIMER_RIGHT_40=1554,20
PRIMER_LEFT_40_TM=58.634
PRIMER_RIGHT_40_TM=60.476
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=929
PRIMER_PAIR_41_PENALTY=2.854628
PRIMER_LEFT_41_PENALTY=2.378922
PRIMER_RIGHT_41_PENALTY=0.475706
PRIMER_LEFT_41_SEQUENCE=AGAACCAACCGATCAACTCTTC
PRIMER_RIGHT_41_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_41=625,22
PRIMER_RIGHT_41=1554,20
PRIMER_LEFT_41_TM=59.621
PRIMER_RIGHT_41_TM=60.476
PRIMER_LEFT_41_GC_PERCENT=45.455
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=930
PRIMER_PAIR_42_PENALTY=2.878667
PRIMER_LEFT_42_PENALTY=2.402961
PRIMER_RIGHT_42_PENALTY=0.475706
PRIMER_LEFT_42_SEQUENCE=ACAAGAACCAACCGATCAACTC
PRIMER_RIGHT_42_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_42=622,22
PRIMER_RIGHT_42=1554,20
PRIMER_LEFT_42_TM=60.403
PRIMER_RIGHT_42_TM=60.476
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=933
PRIMER_PAIR_43_PENALTY=2.884792
PRIMER_LEFT_43_PENALTY=1.504551
PRIMER_RIGHT_43_PENALTY=1.380241
PRIMER_LEFT_43_SEQUENCE=ACCGATCAACTCTTCCACCTC
PRIMER_RIGHT_43_SEQUENCE=CCTTGGGTTTCCTTCAACACT
PRIMER_LEFT_43=632,21
PRIMER_RIGHT_43=1552,21
PRIMER_LEFT_43_TM=60.505
PRIMER_RIGHT_43_TM=60.380
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=6.1000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=921
PRIMER_PAIR_44_PENALTY=2.912322
PRIMER_LEFT_44_PENALTY=2.436616
PRIMER_RIGHT_44_PENALTY=0.475706
PRIMER_LEFT_44_SEQUENCE=ACAAGAACCAACCGATCAACT
PRIMER_RIGHT_44_SEQUENCE=AGCCTTGGGTTTCCTTCAAC
PRIMER_LEFT_44=622,21
PRIMER_RIGHT_44=1554,20
PRIMER_LEFT_44_TM=58.563
PRIMER_RIGHT_44_TM=60.476
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=933
PRIMER_PAIR_45_PENALTY=2.918076
PRIMER_LEFT_45_PENALTY=0.345214
PRIMER_RIGHT_45_PENALTY=2.572863
PRIMER_LEFT_45_SEQUENCE=CCGATCAACTCTTCCACCTC
PRIMER_RIGHT_45_SEQUENCE=TTGCCTAGAAACTCCGGCAC
PRIMER_LEFT_45=633,20
PRIMER_RIGHT_45=1276,20
PRIMER_LEFT_45_TM=59.655
PRIMER_RIGHT_45_TM=62.573
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=644
PRIMER_PAIR_46_PENALTY=2.933909
PRIMER_LEFT_46_PENALTY=2.358553
PRIMER_RIGHT_46_PENALTY=0.575355
PRIMER_LEFT_46_SEQUENCE=CAAGAACCAACCGATCAACT
PRIMER_RIGHT_46_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_46=623,20
PRIMER_RIGHT_46=1552,20
PRIMER_LEFT_46_TM=57.641
PRIMER_RIGHT_46_TM=59.425
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=930
PRIMER_PAIR_47_PENALTY=2.941586
PRIMER_LEFT_47_PENALTY=2.366231
PRIMER_RIGHT_47_PENALTY=0.575355
PRIMER_LEFT_47_SEQUENCE=GAACCAACCGATCAACTCTTC
PRIMER_RIGHT_47_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_47=626,21
PRIMER_RIGHT_47=1552,20
PRIMER_LEFT_47_TM=58.634
PRIMER_RIGHT_47_TM=59.425
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=927
PRIMER_PAIR_48_PENALTY=2.954278
PRIMER_LEFT_48_PENALTY=2.378922
PRIMER_RIGHT_48_PENALTY=0.575355
PRIMER_LEFT_48_SEQUENCE=AGAACCAACCGATCAACTCTTC
PRIMER_RIGHT_48_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_48=625,22
PRIMER_RIGHT_48=1552,20
PRIMER_LEFT_48_TM=59.621
PRIMER_RIGHT_48_TM=59.425
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=928
PRIMER_PAIR_49_PENALTY=2.978316
PRIMER_LEFT_49_PENALTY=2.402961
PRIMER_RIGHT_49_PENALTY=0.575355
PRIMER_LEFT_49_SEQUENCE=ACAAGAACCAACCGATCAACTC
PRIMER_RIGHT_49_SEQUENCE=CCTTGGGTTTCCTTCAACAC
PRIMER_LEFT_49=622,22
PRIMER_RIGHT_49=1552,20
PRIMER_LEFT_49_TM=60.403
PRIMER_RIGHT_49_TM=59.425
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=931
=
SEQUENCE_ID=MAGIC.329_3_10555509_10555545_zu-0_ws-0_51669_481519_TRUE
SEQUENCE_TARGET=950,136
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_10628352_10628493_ler-0_hi-0_248578_6816_TRUE
SEQUENCE_TARGET=950,241
SEQUENCE_TEMPLATE=GGCACTCGAACGATGACTGGTGTTCTTTCTGTGAGTTCCTCAGAAACCGTATACCCCCGTTGAATCCCTTNNANTGNAGTGCNANTNANNTNATTGATTTTCTTCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANCCTTTTCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANACATCTTTCATNGAANCCATNTCAAANGANTTNNNCAANNNNNNNNTCANCCNNNNNNNNNNNNNNNNNNNNNGCAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNGATCTAGNGTNGGTATNATGNTNCTTTCANNTNGTNATGNTTNTNCNAGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTGTNNTTATTCCTATATATTTTAANGNNNNANTTTCAGACATTTGTGGNTTGNAAGGCNGGTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNCAGAAATGGAANGCNGCTNTGNCTGAAATAGNGTCCATCGNTGGANNTNANTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGCCGAGGAAGTTGTAANNAANNNNNNNTTAANNCTNNNTNNNNNNNNNNNCANGAATNTNNNNNNNATCTTANCNTTNTTAAATCANTCCNANTCTNNNGNCGNNGANATTNTGGGNNTNTGGGGTATNGCNGGAATAGGTAAGACATCNATTGCNAGAGAAANATTNGNANTNNNNGCTCCNCANTATGATNNNTGTTACTTNNTNCAAGACTTTNATCTNATGNGTCANANGAAANGGCNNANGCAANTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANNNNAGTTTCANGANGNNNNGGTTCCANAANANAANGNTTNTNNTNNTTCTNGATGANGTGAGNNATGCNAGAGATGNAGAAGNTGTNNTNGGAGGGTTTGGCTGGTTTTCTCNNGGACANANAATNATCNTAACNTCTAGGNNNAANCAAGTTCTTGNANAGTGTAANGTTANANAGNCATANNAGATCCANANATTANNNNANNNNGAATCNTTNCGTCTCTGCAAACAATNNNNNNNNNNNNNNNNNNNNGTCATNTNNGAGCTNATNAGCTGCAGTAGTGGNATNCCATTGGCTCTCNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCTCCAAAGNTTGNGNAAANATCCTCCNACNCAGNTTCANGAAGCNTTTCGNAGAAGNTTTGATGGNCTAGATGAAAACGANAANAACATATTTTTGGANCTNGCNTGTTTTTTNANNNNNNNNNNNNNNNNNNNNNNGGTNNNANTACTTGATGCTTGTGGNTTTNTTACNTATNTGGGAATCTGTGANCTNATNGANGAGTCNCTCATTAGCCTTNTNGACAANANGATAGAGNTNCCTATTCCTTTTCAAGANNNNNNNNNNNNTNTTGTTCNTGAAGAAGANGANGATCCATGNGNNCGTAGNAGATTGTGGGANTNNNNNGACATNNNTGATGTNNTGANNAACAATTNAGTAANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGGAACAGANGCNATTGANGGCATNTTCCTNGATGCNTCTNACTTGACNTGNGAGNTNAGTCCTACTGTGTTTGGTAAGATGTATAANCTTAGATTGCTGAAGTTCTATTGTTCNNCCTCTGNGAANNNNNNNNNNNNNNNTNTACCTCANGGNCTNGACACTTTGCCTGATGANNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_371035_371107_tsu-0_rsch-4_244446_157649_TRUE
SEQUENCE_TARGET=950,172
SEQUENCE_TEMPLATE=CTTATGTGAGTTATATATACAGAGAGATAAAGTGATAGAAGATCTNAGAGAGAGAGAGAGAAAGAGATGGTGATGATGGTGTTATGATGAGGGAATGATGGGGATAAGAGGAAGAGATGGGCTTTTAAGAAGGCTGAAATAAGTCGTCATTCCGAAACCCTAGTCTTGTCTTCTTAGAAAAAAAANCAGNATTTACTCAGCCGACATGTAATTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_1288655_1288749_rsch-4_ct-1_418710_86097_TRUE
SEQUENCE_TARGET=950,194
SEQUENCE_TEMPLATE=TTCATGATGTTTGTTAAGTGTCATTGTTGTAGATGTTGGGGTACAAAAATTGGGTCCTTTGGATGTGTAATTGTTGTTTCATACNGTTGTTAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGCATTGACNAAANNGCCGAAGACGATGAACATTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATANGAAANACTTGNGNATAAATAATAGNAACANTTACGCTNTAACATACTTTTNAAACTTNATTTACATTTTNCCACACTTTNAANTTTTGAANTTTTTTACCCTTNTGTTATTACCNCTATANCCCCAACTAAANNNNATACCNCNCTCTCTCNTTCTAGAATTCATGTCATATGCAGCCNTTGGGCCATTATCCTCGGCAAANTTNGTTACCAATGATGATTTANCNACNCTAANAAAANAAAAAAAANAATTCAATTAAGATGANATATATGAAAAACAACAATGNGTAAGAAAAGAGAANANAAAAAAACTATNATGTCAAAGAAAAAATNATAGAAANANNGGATAATTTANNNTTNNNNNNTTTNAACTAGGCTGAGACGATGANNATTNATCTTGAGAAGANTTTTAANGANTAGAGAGATCTTANGAAAANATAAANTAGAAANATCTTACAAACAANCTANAAANNAAAAANAANCTNTANATCTAAGAAGATGAAAAANNGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTNGTGTCTCGTGTTTTTNATTTNACACAGNTATTAGTNCCCTCCCACANTTATNTTTAATCGTNNNNNACTGTGATTAANTTTNTAGATGAANANCACAATAAATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9475442_9475539_sf-2_po-0_6280_4034_TRUE
SEQUENCE_TARGET=950,197
SEQUENCE_TEMPLATE=GATCAAGNGATCGGATTCACCCNGAACTANANTNAATACANANGAAACTGAAGGAGTTGGAGAAGAAGATGAAACTAGCTGGATACAAACCGGANCTAGAGTTTGCTTTACACAATGTAGAGGAAGNGCANAANGAGAAGCTATTGTTATGGCACAGCGANAAGTTAGCTGTTGCNTTTGGTTGCATAANNNTCCCNNAAGGTTCACNNATACAAGTGTTCAAGAANTTGAGAATCTGTGGTGATTGTCATAANGCAANCAAATTTATNTCGGAGATAGAGAAACGAGAGATCATNGTAAGAGACACCACAAGGTTTCACCATTTNAAAGATGGGTCTTGCTCTTGTGGCGANTACTGGTNAAAANAGAANNGCTTTGACTCTCTCATTGGTCAAACCTGACTGTNTTTANATGCNTTATTGTGTGGTAAAGTTTCGACCTTTGACTTNACAAGTTGNCGTTAAGNAGAGAGATGCGTAGATCAGCGAGTGGTTCNAGAGTTTTGGATNATTTTCCNNCGACTTNANGGTCTCCNCNTCGATCTCAGAGNNTTNCANCNNTGGAAGATGATGNGGAGNTNCTTTTGCCTAGGTACGATCCGAATTCNCAAGCGGGGAAGAGAGAGAANTNAAGATTCAGATTTNCAGAAAACGTCATCCATTTGATTCCTCTCATTCTTCTTCTCTGTNTNGCAATCCTCTGGCTCTCCTCTTANTCAGGTNANCCGANANATTGNTTCAATCTCTATNAATCCATAATTGATNTGTGAAACTTAATTAGGNATTTNACAAAGNCTCATATNNATNTNNNNNNNNNNNNNNNNNNNNAANNTTNGAATCTTGTNTTNAANNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAANCNGTGATGGATNTGGAATTTGTCAGCAGCNTTNAGGAGTTNANTTCNANAAGCAANATGTTGTCTTGTCTCCATGNNANNTCNTATTNNGTTTTGGGAAAGGAAACANNNNTTTTACNNCCGGTGATTNTGTGCCGCAAACCATNCNNNTTNNNNNNNNNNNNNNCTNTAGACACANNAAANGATCTCTCGTTTTCATGANNTGTATGTTTAATANTTNANNNNNNNACATCANACAANTCANGTANAANNCACTGATGGTNATCNANTNNTCATTCTATTNGTCNAAANAANGNTNANTTTCAACTTNATNCNNCNTNANGATTANATGTNCNTNNNNNNNTCAGCTAGCCAANNCAACNANAGTNAAACAATGNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAANTNNTNTAATANTCACTNTACTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTTNAANTANTTANTGNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTNAAANTATGGGNNNNAGGCCCANACTTNANNAAAANNTTGAAANNCAAAGNNNNNNTNTNACTTNTNTTCTNTCTCANTGAATATCTCCCAATCAAGCTTCTTNNNTTTTGNNNNNNCTCTCTCTTACACNGCCANTCNNTGTTTTCNCAGCTTTCAGGTTTGTCTCAATCTCAAATNAAATCGGAGTCAAGTAATAACANTTGATAANCCTAATTGNTTCNATTNTATTGTAAGANTNGAANNTTTGNNGTAGATCCGNAATCGNATTCTAGTTCTGGAATCGTTGATCTCGATNNNNTTTTTTNNAAGATTTCTTCNNACACATTTGGTTCAAAAGATCACATANNTTTNNTTTAATTTGATAAGTNTGATGATTCTGCTAAGTGGCATTGGANAAAGTTTTCNNTTTTGCAATNCGNCTAAACTTGTCTNTGTCTTGAATGAACTCTCTGAGTTGCTTAAAANNTCTTGTGCTTTCTTTATTACAC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_13468898_13469040_wil-2_ws-0_5176870_4421_TRUE
SEQUENCE_TARGET=950,242
SEQUENCE_TEMPLATE=NATTTGGCNGCTAGAGACTTGAGATGTTGGTGNGTGTAGTNGAGAATAAATAGCCTGGAAACTCACATTGACAGACCAATCNAAAGNAAATTCACTGACNCGTGTAAAGCTCTGAGCATCGAATACNCNNATTTTACAGTCGTTATTTGCGGTTATAAGCCTAAGGGAGCCACTGCAANAAGNAGAGAAGAGAAGAAAATTTAGCTCGAGTTCAGGATGATANGTAAAACCTCTANAAATTGCTNATAACNCTCTGGCCCTCTTTATANNNTACTATACCTTGGGTCTCGNTATATATCAACTGTGTTTGTGATGGCATTATCTTCCGTTGATAATCTTGTGCAAAAAGCAACCCCAGGCTGATTTACANACTGGAGAAAGAGNANAGCAATACAAGTTAAACGATATAGGTTTTAAGGTTTATATTATCTACTNGNCNAAAGCAATGACTATTCGAAGAAAATGTGAACCTTGCAGAGAAGCTCCCCGTCGAACCCTCCTAAAAGGATCAGATTTTCTTTGACTGCCATGCTGCTAACTTGCACCCTAGAGAGTGNCTCAGAAAACAATCCAGGTAGTTTCTGAAANAAAAATNAAACNAGGANCTAAGANCAATCTGGAAAANTATANNTTTGNAATAGTTTTCATGNNTTNCNACNGCTCTCACCTGAGTGGGGGTAACTGATCTGGCGACATTAANTACTTCTTTCCCTCTNTGNAGTAGAGATGACCAGTGCATGAGTGAATANTTTTGCATTANATATACATCATGCTTNGATGTCGCCCATACCAAATTCCGAAGCTGTNAAAGNAANAATATGAANGNTCAATCACGCGCCATATACGCAGANAACATGAAAACAAGATTTTCTTGTATATGAATATGAGGTANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTAGANACTAAAATGANCAATGTTGTTTTACACAAAATGATTAAGTTNCANCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGANAAGTTAGTGNATGAATGAGTAAAGNACCNAAAACTNCTCAAGNTTNACAAANTATATGCATGNNNNNNNNNNNNNTNTNNTNNGAAANNNNNNANNNTNTNNGGNNNNANGGNNNNAACATTCTCCTATCATACCCATATCGTTATGCTGNNGGCTGCTCAGACNNTACTCCCAACTGCATCAGNGGTTNAAATTAGAGGAAAATANCCCNAANTNNCACAAATTATTGAGTNATATGTTNATAAGCCATNNTCNNATTAGAATAAACACACCNCANATCTGCANNANAGGATNTATGCAAANTACAGTGCANAAACGATNNNGTATATGATTNTTCNAACCNTCACTTTATTNANNNAANGTNCNTNANNACTTTAAANCCCNACNCTANTTTCATANTTAANTCTGTNNTTTTGTTNGATNTTGGCTGATAAAGTCNCTTGATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNATACAAGCATCCNCCGAGANCTCTGCNGAAAAAGGATTANAGNGAATAGAAAACCNGAGNGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGAGGATAGTATCACCATGGATGNTGGTANTCATGNANTGTACTCAGAGANCAATNTAGNCAACCTGAAGCANNTTCTTAGACAAAAGNTACCTGCNCATNTANNTNTNNNNNNNNNNNNNNNNCATTTTGNAANTCATAGNTTTTTCAAATGANATGCAATTTAACNTATGCTNTAATTATCTACCAACTAAACCANTATTNAACANAANNTTNANTNAATCCAATAATGATGCAAATGANCNGCNTATNAACTCTTAAANTNNAAGAAAGATTCATCTTTTANANNNNNNNNNNNNNTGAATNAGCAGANACAAAANCTNTACCTGAAAATGCACAATAGTGGANGTGACAAGCCTTGTATTGAANTGGAAGTCATAAAAGTTCTTNCCTTTCTCANNNACTTGNCNATGTTCCTGTCNATTNTAACANNNNNNNNNNNNNNNNNNNAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_6943186_6943201_wil-2_edi-0_125777_13118_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=CTCTAATTATCCTTAATAATTTTTCNTTTTATATGACATCTCTCTTAAAATCCTAGGTTAGAGACTCGCATTGTGCAGCGTGGAGGAACATTTGTTAAATTTTTCATGCAGTATTTTACANATTTGTGATATGATATAAAATAAATACCTTTTTGANNAAAAAAAATTAGATGATGTTGATATATGGTGNNNNNNTAGTATNATATANTNNNNNNTATATNTATANNNNATANTATANATATANGTATATNTTTTNTAACAATNTTTTTTATTCTATTATCGATAGTTGGGTTTGATACTAATATCCNCNTCTTCACTCCATNTGTATCACACCAACCACAAAGAAAGAATACTCTTGGTTTTTACATCGAGAATAGATAAGAATCGGGTATCNGTGCCAATTTNTCGTTAACCACCAANCTTGTTAACCTCATCGTTNCTCANATATTACATGANCCNTAAACAACTAGATTAGTACANTACNAATAGTACTTGATNATAATAAAAAANGACAACTATATAATTATATAAAACAATNTANANCTGTANNATTTANTCGGTAAGTTTTNNTTTTTTGGGGTNTAATGTANAGATTTATATCAAATATAAAANGTTTACATTTAACAATGAAACCCAAAAAANACACTTANGACTTTAAGNTCACAACACNCNTATNTNGCTANCTTCAAAANGGAAACCCTACATCTCTTTNTNNATTCTAAATAAGNCTTTCTCCAAGCAAANTTTTTTTTTNGNTTTTTTATCATTTAACANTTNTTNTGATCANTGAGAAGTGNTCNGGTAANCACTTTTGANNAAANTAANGAGTAGGAAANTCNTGTTCNTCNTTNTTCCTNAATTTGTTTTTACCANTTTNCAANNAANTTTTGTTCNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTCCTCATCATTCCTCANNNGAAANGAATGGTAGAACANAATNGTTNCTNCCCAAAATTGTNNNNNNNNNNAGAGGAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_15800681_15801225_wil-2_zu-0_14261_81781_TRUE
SEQUENCE_TARGET=950,644
SEQUENCE_TEMPLATE=ACATGGACCTCGGCNAGNAATATCTCCTCTCTACAATTATGATCTCANTTTGTGACAGATTNTANGNATTTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTAATCANATGGGNTTTTGGNAGANTCTTGNGATAGNTAATAACAAGCAAATTCTTCTTANTCAAATTTGACTGANANAACAAAAATACGAGAATAGCCAAAACATCATTNATCCAAAATAAGANATGCTTAANGANGTTCAACAACTTAATTAACTCCCAAAATAACAATGGTTCTCNAAAGTAAAAANTTNAAAAACTACAANAGCTTNAAAGCCANACCCATNGANGCCTTCATGTTTATATCANTAAGCATCAATCATGAAGTTGGTGCTTGATTAACGGTACTGTAGAAACGAGAAAACAAAATCAGAAACAACAGTNTTGTGTAATGATGTTAACACAATTCATAGAGTTTGGAATTTACCTTAATGAATCCAATCTCCTTAGCGTTGCTACGGAAACACTGTCTGCAGCAGTTCAAACCATACTTCCNGATCAGNCCGTGCGAGTTNCCGCACACACGGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACGATCAGATCCATTAGAGNNNNNNNNAATGGTGTTCAAGTTCNATACTTCTAAGANCCAAACAAATCAAAGCAAAACGCGAGAGNTAGANGAAGATGATTATACCATAAACGAGATCCAGGACCGTACTTCTTNGGATGAGAGTTCCANACATTAGAGTGACCCNTTTNGNCGATTTGCTNACAGAGGAATNACAAACGGCGACGAGAGANACNCTTCAAAACCCTAGAAATTTAGTCNNNNTGANGAAGNNGCTGAGNTTTTATAACTNGTNCANTTTTTATCTAAANNGGCCNNNNNNNNNNAGGCCCNTTAATTGGNGGNTTNNTTTTTTTNTNTNNTTCTTCTCCATCCACATGTTCTTTGCTTTGAATGATCNTNNNNNNNTTTTTTTTTTTTTTTTTTNGAGTGAATCCATGGCCANTCGACCTCTCATCTCTCTGCAACTTCNNNNNNCTTCTTCTTCATNTTCCTCGTTAAAANCAACAACAAAACTTNTCTTCTNTCATAAACCTGAACTAATTAGCCACCACCATAGAAACCGTAACAAGTTGTCNCGNACAAGNGNTCATCAANNNTCAGCTAATCTCATCATTCANCCTTCTGTTCTTTTCCTAAGTAGCGCCTTCGATGGCGGTGGAGGATTCATCGATACACAGACTTTNATTGTCACCATTAGTCTCGTTGTAGCCATTGCTCTCTCNCTATTCCTCGGCTTCAAGGTTTNGTATCNATTATTCTTCTCTACTTAATCGTTCAATAAGTNATTTTGGTGNGNTTTTGATCTTTTTANTGTCTTATTGATTTGTTTCAGGGAGATCCAGTTCCNTGTGAGAGATGTGGTGGAAACGGTGAATCNTTTACTCTGTCTCTCTNTGTTTGCATTATGATTAGNANATGTCTTATTAANTTTTTAGTCACCTATANCANGAAATCAAAATCCATTAGGTTNTATGTAANTTATNTTNNAAAAAATTNNAAACTAGTTTTACTNTGTAGCATGNTTGTCTTTNGATTGGATACTTATGCATTGNNCAATGNAAGTAAACAATGTANACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_5423755_5424246_bur-0_po-0_13985_353617_TRUE
SEQUENCE_TARGET=950,591
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNCCAAATTCNNGAGANAATCGAGATCGAATTGTTTAAGAANCTTCTNCNNAAGTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGTATANATTNGCATANNAATTAGNNAGAGNTTATTCGNAGAAAGATGCATTCNAGTTTCTATTGNAGCAACTTCACANNGTTTATCTCTAGNCTTAAGCATCATGTAAGTCGTTTTGTATTTTTCAAAAGATTGATTTNTGTNNNTATATATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTCTAGTGTNGCAAGTNGGTGGAGAAAANATAANTATATGTNTNTATGTGTGTGTTNNGTTNTGTTGTTNTGTGAGATNAAAAGTTATTAACAATGTAGACAATTNNAAGANAAAATCGTTTNTTGTGTTTCTCTCTAANAAGAACTACAAGAGAGTGCAACAATAANAGAGAGACATAGTTAAAGAATGAGAGATCTAACCTCTAGGAGTGATAAAGAGGTTAGATGTGAGAATGTCAACACTGTNTTTNTGTTTACGTNNTCGATNTCTTGTAGTTTCTATTGTACAAGCANATCNCTTAAANTTGGTCATGTTATATCTTCACGCAAAGGATCTTCGTTCANGAGATTTTGNCTTNCCTCANTAATTNTTAATCATGGGTTTAATGGNTACTATAGTCTACCNTATTCTTTATTACCAAATAATANTGTTAGTTTGACTAAAANTTCAGATCAAAAGATTTTGTGGACATGNTTAATTTNGGTAAATATCTAGTAATAGTGNATATGGATTGNNACTTTGNAGTAGTTAATNAGGATANCAAAAATTACTANCNNNNNNNNAAAAANTTANNCATAATTTTNAGAAACTTTTTNTAATACATAGAAAGTAATCNNTTTTTTGCTGAACATACNTAGAAAANANTNNNNNNNNNNNNNNNNNNNNNNNNNNCTTNTATCNNNNNNNNNNNNNAANNNNNTAGTNNNNNNNNNNNATTATTGCAAAAAANAANNAAGCCTTTGNAATGTTGCNCACCGAAAACCCTACCTCTAGNCCCTATCTGATTCCCNAGAATTNNATCAAAGCAACTTGNATTCGATAATCTCCTCCTTGNTTACATTTCNGGATTCTCCATCTNTCTCCATAAGCTCNTCTTTNGATCGAGTACTTGATAATCTTCTCTCCTCTTGTGATGTCTCCGTTCAAGACNAACTCNTCNATCGTACTNTCGAGCGACTCTCNNTTCTTCTCCAATCCACCAAACGNTGCTCTNANAAACNAGCCACACTCCNCAATTCCATNTCNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_6925725_6926217_wu-0_can-0_121083_16876_TRUE
SEQUENCE_TARGET=950,592
SEQUENCE_TEMPLATE=TTAAGAATATGAATCTCGTAGCATCATANTCAATCTCACTCCTTTTATATTCTAAGAGATTCATCCAGAAATCGCTGTTCTGCTAACTAAGCAGNAATTATCTGAGGATTCATNGAGAAGCGACTAAACATGAACAAGTGAGGGAGATAACCAGTGAAGCAGAGACTCAATTTAAGAATTTAGCAAGAAGTAGAGGGAGAGCGTAATCNTACCGCCGGAAGAGGTGGGGAAGTGCACGGCGNCGATGCGGAGGAGGAGATTAATAGATGGGGGACAAAGAGATATCACANATATCAGTGAATGAACGGTGAAGGAGGAAACAAAAATAAAACTCTTAAAACTTTGCGTATAGTTTTCATTTTCGCTNNNNNNNNNATATCATAAGATTTGCTAAAAATTATAGTAGAACNANAAAAAAAGATAGCATGNACAAAAACGTCTTAAATTAAAATACATGACTTATAAAAAGANAAAAAAAAACACATATTTATCCAAATTAGTTTAGATAAATATNATATTTAATTTTAAAATTGCTATGATANAAAAAANATATATAAATTTGGATTNAATAGTCAACAATTATGATGAATTAATTTTCGAGATAAAANTAAATAATTAAATATTGGATAGGTTATCAGAATAAACAAAANTAAGCCAAAGTTTTTTTCCATTAAAGTCAAGGTTTTGTAACTTGCACGCTTCAGCGAAGAAACGGTGGCGTCTCGGCNCTTCCACACATTGACACATAATATCTACTTGGTCTTTTGCTCGTCTCATTATCTGAATTTTTTTCTTNCAGCATCGACACATGAGATTTCAAGCTAAACCATCTTACATATCTTTTTGTTTTTGTTTTAATTAAATAAAATACCAAATTGATTCGACATTCCAATATGTAAACCAACATACCTGAATACNCNAAACTCTTTTGACTCAAAATCGAAGTTTTCTNTNATTTTTCTCAACAAGCAGAACTTATTTAAATCTCATACGTTTATAANATATAAACTGNCTTGATTAATAATATAGATCTACTATATCTTGTTAGCTTGTGAAAGAAATATTTGGATGCACAAAGATTAAGGTCTTGAATGGTTGTTNAAGCCATTCCAAATNTGACCAAAGTTAGACCAATCTTTTTTTCTTCTACCATTTAAACTGATCTACACAGGACGAATACAATGTAGATGAGTACACAAATCNCAAAGAACAACNATTACATACCANAATATCACTATCTATNTTTTNATTTCNTNTNCTTTANAATTAAAACGTACTCCAATATANAGTTTAGCATACCATAATATCGGTATGGTTGGTNTAGTATATACCACATAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNGGATTAACACATACTACCTANTGACATTATAAACGGAAAGGNGGAGGTGTAAATGATAATAAATATATATTAGTCGGTGAGTTTAGAAAGAAACAANAATTANAGGGGTGTGATTTCGAAGAAAATNTTGCATAGTTTTCAAANTTTGAAAATCTCAGCTAAAGAGAGAAAGAGAAGCACCATTTTTCTTTCTCGTCGTGTCATCGTTTTCTCAGATTCCTGAATAATCATTCCCTTCCGATTCCAAAATCCCCAAACCTCATCCACTCCTCCATCTCCCGGATTCGGATTCCCAGAATCATGCCCATGGAGAACGACAATGGCCCACACGTTGGCAACGTCGTCGTCACCGCTGAGCAAGCGACCAAGATTAACGAGACTGANGGACGGTTGCCGGAGAATCGGCAGACCGGTGTTGTCTCTGACACCGGAAGTGGTAGCGAGAGAGGAGAGCAAGGGGTTGGAGAGNCGGCAGTTGCTGTGGCGGTGCCGGTGGAGGAGAGCGGATCGATTTCGGTGGGAGAGCTACCTGCTCCCCGATCTTCCTCTGCTAGAGTTCCGTTTACTAATCTAAGCCAAATTGATGCGGATCTTGCTCTTGCTCGCACACTCCAAGAACAGGTGCGTCTTTCTTTCTCATCTCTCTATTTCGGATTTCTCCGTTTTTAGGTTATTCCATGCTTGTTAGGGCTTTGTTAGTGCTACTTCTCCGGCATAGGNGAAAATCTTAGGGCTTTCTTTCTAANTGCATTTGCAATGTTATTATGTAGGATTGTCTATTGAAAANTTGTTGTTTTGTATGTGTGCGTGTATGTTTCGGGNTTCAGGAGCGGGCGTATATGATGTTGACCATGAATAGTGAAATCAGTGACTATGGGAGCTGGGAAACTGGAAGCTATGTATACGATGAGGATGAGTTTGATGACCCCGAGAATGAGGATGAGGATGATGATGAAGATGAATATGAAACAGATGATGATCCTCAGGAAGATGGGCTTGATGTCAATGTGCACGCCAATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.438382
PRIMER_LEFT_0_PENALTY=0.434141
PRIMER_RIGHT_0_PENALTY=0.004242
PRIMER_LEFT_0_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_0_SEQUENCE=GGATGAGGTTTGGGGATTTT
PRIMER_LEFT_0=756,20
PRIMER_RIGHT_0=1736,20
PRIMER_LEFT_0_TM=59.566
PRIMER_RIGHT_0_TM=59.996
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=981
PRIMER_PAIR_1_PENALTY=0.441711
PRIMER_LEFT_1_PENALTY=0.434141
PRIMER_RIGHT_1_PENALTY=0.007570
PRIMER_LEFT_1_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_1_SEQUENCE=GGAGATGGAGGAGTGGATGA
PRIMER_LEFT_1=756,20
PRIMER_RIGHT_1=1750,20
PRIMER_LEFT_1_TM=59.566
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=995
PRIMER_PAIR_2_PENALTY=0.524296
PRIMER_LEFT_2_PENALTY=0.434141
PRIMER_RIGHT_2_PENALTY=0.090155
PRIMER_LEFT_2_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_2_SEQUENCE=TTTCTTCGAAATCACACCCC
PRIMER_LEFT_2=756,20
PRIMER_RIGHT_2=1595,20
PRIMER_LEFT_2_TM=59.566
PRIMER_RIGHT_2_TM=59.910
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=840
PRIMER_PAIR_3_PENALTY=0.907835
PRIMER_LEFT_3_PENALTY=0.434141
PRIMER_RIGHT_3_PENALTY=0.473695
PRIMER_LEFT_3_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_3_SEQUENCE=TTCTTCGAAATCACACCCCT
PRIMER_LEFT_3=756,20
PRIMER_RIGHT_3=1594,20
PRIMER_LEFT_3_TM=59.566
PRIMER_RIGHT_3_TM=59.526
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=839
PRIMER_PAIR_4_PENALTY=0.928951
PRIMER_LEFT_4_PENALTY=0.434141
PRIMER_RIGHT_4_PENALTY=0.494811
PRIMER_LEFT_4_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_4_SEQUENCE=GAGGAGTGGATGAGGTTTGG
PRIMER_LEFT_4=756,20
PRIMER_RIGHT_4=1743,20
PRIMER_LEFT_4_TM=59.566
PRIMER_RIGHT_4_TM=59.505
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=988
PRIMER_PAIR_5_PENALTY=0.928951
PRIMER_LEFT_5_PENALTY=0.434141
PRIMER_RIGHT_5_PENALTY=0.494811
PRIMER_LEFT_5_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_5_SEQUENCE=GGAGGAGTGGATGAGGTTTG
PRIMER_LEFT_5=756,20
PRIMER_RIGHT_5=1744,20
PRIMER_LEFT_5_TM=59.566
PRIMER_RIGHT_5_TM=59.505
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=989
PRIMER_PAIR_6_PENALTY=0.928951
PRIMER_LEFT_6_PENALTY=0.434141
PRIMER_RIGHT_6_PENALTY=0.494811
PRIMER_LEFT_6_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_6_SEQUENCE=TGGAGGAGTGGATGAGGTTT
PRIMER_LEFT_6=756,20
PRIMER_RIGHT_6=1745,20
PRIMER_LEFT_6_TM=59.566
PRIMER_RIGHT_6_TM=59.505
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=990
PRIMER_PAIR_7_PENALTY=1.047896
PRIMER_LEFT_7_PENALTY=0.434141
PRIMER_RIGHT_7_PENALTY=0.613756
PRIMER_LEFT_7_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_7_SEQUENCE=ATTTTGGAATCGGAAGGGAA
PRIMER_LEFT_7=756,20
PRIMER_RIGHT_7=1721,20
PRIMER_LEFT_7_TM=59.566
PRIMER_RIGHT_7_TM=60.614
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=966
PRIMER_PAIR_8_PENALTY=1.136959
PRIMER_LEFT_8_PENALTY=1.132718
PRIMER_RIGHT_8_PENALTY=0.004242
PRIMER_LEFT_8_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_8_SEQUENCE=GGATGAGGTTTGGGGATTTT
PRIMER_LEFT_8=757,21
PRIMER_RIGHT_8=1736,20
PRIMER_LEFT_8_TM=59.867
PRIMER_RIGHT_8_TM=59.996
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=980
PRIMER_PAIR_9_PENALTY=1.140288
PRIMER_LEFT_9_PENALTY=1.132718
PRIMER_RIGHT_9_PENALTY=0.007570
PRIMER_LEFT_9_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_9_SEQUENCE=GGAGATGGAGGAGTGGATGA
PRIMER_LEFT_9=757,21
PRIMER_RIGHT_9=1750,20
PRIMER_LEFT_9_TM=59.867
PRIMER_RIGHT_9_TM=60.008
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=994
PRIMER_PAIR_10_PENALTY=1.213879
PRIMER_LEFT_10_PENALTY=0.434141
PRIMER_RIGHT_10_PENALTY=0.779738
PRIMER_LEFT_10_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_10_SEQUENCE=GATGAGGTTTGGGGATTTTG
PRIMER_LEFT_10=756,20
PRIMER_RIGHT_10=1735,20
PRIMER_LEFT_10_TM=59.566
PRIMER_RIGHT_10_TM=59.220
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=980
PRIMER_PAIR_11_PENALTY=1.222873
PRIMER_LEFT_11_PENALTY=1.132718
PRIMER_RIGHT_11_PENALTY=0.090155
PRIMER_LEFT_11_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_11_SEQUENCE=TTTCTTCGAAATCACACCCC
PRIMER_LEFT_11=757,21
PRIMER_RIGHT_11=1595,20
PRIMER_LEFT_11_TM=59.867
PRIMER_RIGHT_11_TM=59.910
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=10.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=839
PRIMER_PAIR_12_PENALTY=1.344167
PRIMER_LEFT_12_PENALTY=0.434141
PRIMER_RIGHT_12_PENALTY=0.910027
PRIMER_LEFT_12_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_12_SEQUENCE=ATGAGGTTTGGGGATTTTGG
PRIMER_LEFT_12=756,20
PRIMER_RIGHT_12=1734,20
PRIMER_LEFT_12_TM=59.566
PRIMER_RIGHT_12_TM=60.910
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.8000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=979
PRIMER_PAIR_13_PENALTY=1.382071
PRIMER_LEFT_13_PENALTY=0.434141
PRIMER_RIGHT_13_PENALTY=0.947930
PRIMER_LEFT_13_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_13_SEQUENCE=AGATGGAGGAGTGGATGAGG
PRIMER_LEFT_13=756,20
PRIMER_RIGHT_13=1748,20
PRIMER_LEFT_13_TM=59.566
PRIMER_RIGHT_13_TM=59.052
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=993
PRIMER_PAIR_14_PENALTY=1.384002
PRIMER_LEFT_14_PENALTY=0.434141
PRIMER_RIGHT_14_PENALTY=0.949861
PRIMER_LEFT_14_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_14_SEQUENCE=GTGGATGAGGTTTGGGGATT
PRIMER_LEFT_14=756,20
PRIMER_RIGHT_14=1738,20
PRIMER_LEFT_14_TM=59.566
PRIMER_RIGHT_14_TM=60.950
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=983
PRIMER_PAIR_15_PENALTY=1.489993
PRIMER_LEFT_15_PENALTY=0.434141
PRIMER_RIGHT_15_PENALTY=1.055853
PRIMER_LEFT_15_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_15_SEQUENCE=AGATGGAGGAGTGGATGAGGT
PRIMER_LEFT_15=756,20
PRIMER_RIGHT_15=1748,21
PRIMER_LEFT_15_TM=59.566
PRIMER_RIGHT_15_TM=59.944
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=993
PRIMER_PAIR_16_PENALTY=1.534666
PRIMER_LEFT_16_PENALTY=0.434141
PRIMER_RIGHT_16_PENALTY=1.100525
PRIMER_LEFT_16_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_16_SEQUENCE=GATGGAGGAGTGGATGAGGT
PRIMER_LEFT_16=756,20
PRIMER_RIGHT_16=1747,20
PRIMER_LEFT_16_TM=59.566
PRIMER_RIGHT_16_TM=58.899
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=992
PRIMER_PAIR_17_PENALTY=1.560280
PRIMER_LEFT_17_PENALTY=1.556038
PRIMER_RIGHT_17_PENALTY=0.004242
PRIMER_LEFT_17_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_17_SEQUENCE=GGATGAGGTTTGGGGATTTT
PRIMER_LEFT_17=755,21
PRIMER_RIGHT_17=1736,20
PRIMER_LEFT_17_TM=60.556
PRIMER_RIGHT_17_TM=59.996
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=982
PRIMER_PAIR_18_PENALTY=1.561691
PRIMER_LEFT_18_PENALTY=0.434141
PRIMER_RIGHT_18_PENALTY=1.127550
PRIMER_LEFT_18_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_18_SEQUENCE=GATTTTGGAATCGGAAGGGA
PRIMER_LEFT_18=756,20
PRIMER_RIGHT_18=1722,20
PRIMER_LEFT_18_TM=59.566
PRIMER_RIGHT_18_TM=61.128
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=967
PRIMER_PAIR_19_PENALTY=1.563608
PRIMER_LEFT_19_PENALTY=1.556038
PRIMER_RIGHT_19_PENALTY=0.007570
PRIMER_LEFT_19_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_19_SEQUENCE=GGAGATGGAGGAGTGGATGA
PRIMER_LEFT_19=755,21
PRIMER_RIGHT_19=1750,20
PRIMER_LEFT_19_TM=60.556
PRIMER_RIGHT_19_TM=60.008
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=996
PRIMER_PAIR_20_PENALTY=1.606412
PRIMER_LEFT_20_PENALTY=1.132718
PRIMER_RIGHT_20_PENALTY=0.473695
PRIMER_LEFT_20_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_20_SEQUENCE=TTCTTCGAAATCACACCCCT
PRIMER_LEFT_20=757,21
PRIMER_RIGHT_20=1594,20
PRIMER_LEFT_20_TM=59.867
PRIMER_RIGHT_20_TM=59.526
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=10.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=838
PRIMER_PAIR_21_PENALTY=1.625049
PRIMER_LEFT_21_PENALTY=0.434141
PRIMER_RIGHT_21_PENALTY=1.190908
PRIMER_LEFT_21_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_21_SEQUENCE=ATGGAGGAGTGGATGAGGTTT
PRIMER_LEFT_21=756,20
PRIMER_RIGHT_21=1746,21
PRIMER_LEFT_21_TM=59.566
PRIMER_RIGHT_21_TM=59.809
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=991
PRIMER_PAIR_22_PENALTY=1.627528
PRIMER_LEFT_22_PENALTY=1.132718
PRIMER_RIGHT_22_PENALTY=0.494811
PRIMER_LEFT_22_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_22_SEQUENCE=GAGGAGTGGATGAGGTTTGG
PRIMER_LEFT_22=757,21
PRIMER_RIGHT_22=1743,20
PRIMER_LEFT_22_TM=59.867
PRIMER_RIGHT_22_TM=59.505
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.9000
PRIMER_RIGHT_22_END_STABILITY=8.8000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=987
PRIMER_PAIR_23_PENALTY=1.627528
PRIMER_LEFT_23_PENALTY=1.132718
PRIMER_RIGHT_23_PENALTY=0.494811
PRIMER_LEFT_23_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_23_SEQUENCE=GGAGGAGTGGATGAGGTTTG
PRIMER_LEFT_23=757,21
PRIMER_RIGHT_23=1744,20
PRIMER_LEFT_23_TM=59.867
PRIMER_RIGHT_23_TM=59.505
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=1.627528
PRIMER_LEFT_24_PENALTY=1.132718
PRIMER_RIGHT_24_PENALTY=0.494811
PRIMER_LEFT_24_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_24_SEQUENCE=TGGAGGAGTGGATGAGGTTT
PRIMER_LEFT_24=757,21
PRIMER_RIGHT_24=1745,20
PRIMER_LEFT_24_TM=59.867
PRIMER_RIGHT_24_TM=59.505
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=989
PRIMER_PAIR_25_PENALTY=1.646193
PRIMER_LEFT_25_PENALTY=1.556038
PRIMER_RIGHT_25_PENALTY=0.090155
PRIMER_LEFT_25_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_25_SEQUENCE=TTTCTTCGAAATCACACCCC
PRIMER_LEFT_25=755,21
PRIMER_RIGHT_25=1595,20
PRIMER_LEFT_25_TM=60.556
PRIMER_RIGHT_25_TM=59.910
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=10.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=841
PRIMER_PAIR_26_PENALTY=1.701634
PRIMER_LEFT_26_PENALTY=0.434141
PRIMER_RIGHT_26_PENALTY=1.267494
PRIMER_LEFT_26_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_26_SEQUENCE=CGACGAGAAAGAAAAATGGTG
PRIMER_LEFT_26=756,20
PRIMER_RIGHT_26=1669,21
PRIMER_LEFT_26_TM=59.566
PRIMER_RIGHT_26_TM=59.733
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=914
PRIMER_PAIR_27_PENALTY=1.713141
PRIMER_LEFT_27_PENALTY=0.434141
PRIMER_RIGHT_27_PENALTY=1.279001
PRIMER_LEFT_27_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_27_SEQUENCE=AGGAGTGGATGAGGTTTGGG
PRIMER_LEFT_27=756,20
PRIMER_RIGHT_27=1742,20
PRIMER_LEFT_27_TM=59.566
PRIMER_RIGHT_27_TM=61.279
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=10.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=987
PRIMER_PAIR_28_PENALTY=1.746473
PRIMER_LEFT_28_PENALTY=1.132718
PRIMER_RIGHT_28_PENALTY=0.613756
PRIMER_LEFT_28_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_28_SEQUENCE=ATTTTGGAATCGGAAGGGAA
PRIMER_LEFT_28=757,21
PRIMER_RIGHT_28=1721,20
PRIMER_LEFT_28_TM=59.867
PRIMER_RIGHT_28_TM=60.614
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=965
PRIMER_PAIR_29_PENALTY=1.763451
PRIMER_LEFT_29_PENALTY=0.434141
PRIMER_RIGHT_29_PENALTY=1.329311
PRIMER_LEFT_29_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_29_SEQUENCE=GATGGAGGAGTGGATGAGGTT
PRIMER_LEFT_29=756,20
PRIMER_RIGHT_29=1747,21
PRIMER_LEFT_29_TM=59.566
PRIMER_RIGHT_29_TM=60.329
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=992
PRIMER_PAIR_30_PENALTY=1.875616
PRIMER_LEFT_30_PENALTY=0.434141
PRIMER_RIGHT_30_PENALTY=1.441475
PRIMER_LEFT_30_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_30_SEQUENCE=TGGATGAGGTTTGGGGATTT
PRIMER_LEFT_30=756,20
PRIMER_RIGHT_30=1737,20
PRIMER_LEFT_30_TM=59.566
PRIMER_RIGHT_30_TM=61.441
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=982
PRIMER_PAIR_31_PENALTY=1.912456
PRIMER_LEFT_31_PENALTY=1.132718
PRIMER_RIGHT_31_PENALTY=0.779738
PRIMER_LEFT_31_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_31_SEQUENCE=GATGAGGTTTGGGGATTTTG
PRIMER_LEFT_31=757,21
PRIMER_RIGHT_31=1735,20
PRIMER_LEFT_31_TM=59.867
PRIMER_RIGHT_31_TM=59.220
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=2.029733
PRIMER_LEFT_32_PENALTY=1.556038
PRIMER_RIGHT_32_PENALTY=0.473695
PRIMER_LEFT_32_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_32_SEQUENCE=TTCTTCGAAATCACACCCCT
PRIMER_LEFT_32=755,21
PRIMER_RIGHT_32=1594,20
PRIMER_LEFT_32_TM=60.556
PRIMER_RIGHT_32_TM=59.526
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=10.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=840
PRIMER_PAIR_33_PENALTY=2.042744
PRIMER_LEFT_33_PENALTY=1.132718
PRIMER_RIGHT_33_PENALTY=0.910027
PRIMER_LEFT_33_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_33_SEQUENCE=ATGAGGTTTGGGGATTTTGG
PRIMER_LEFT_33=757,21
PRIMER_RIGHT_33=1734,20
PRIMER_LEFT_33_TM=59.867
PRIMER_RIGHT_33_TM=60.910
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=8.8000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=978
PRIMER_PAIR_34_PENALTY=2.048790
PRIMER_LEFT_34_PENALTY=0.434141
PRIMER_RIGHT_34_PENALTY=1.614650
PRIMER_LEFT_34_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_34_SEQUENCE=ATGGAGGAGTGGATGAGGTT
PRIMER_LEFT_34=756,20
PRIMER_RIGHT_34=1746,20
PRIMER_LEFT_34_TM=59.566
PRIMER_RIGHT_34_TM=58.385
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=991
PRIMER_PAIR_35_PENALTY=2.050849
PRIMER_LEFT_35_PENALTY=1.556038
PRIMER_RIGHT_35_PENALTY=0.494811
PRIMER_LEFT_35_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_35_SEQUENCE=GAGGAGTGGATGAGGTTTGG
PRIMER_LEFT_35=755,21
PRIMER_RIGHT_35=1743,20
PRIMER_LEFT_35_TM=60.556
PRIMER_RIGHT_35_TM=59.505
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=8.8000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=989
PRIMER_PAIR_36_PENALTY=2.050849
PRIMER_LEFT_36_PENALTY=1.556038
PRIMER_RIGHT_36_PENALTY=0.494811
PRIMER_LEFT_36_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_36_SEQUENCE=GGAGGAGTGGATGAGGTTTG
PRIMER_LEFT_36=755,21
PRIMER_RIGHT_36=1744,20
PRIMER_LEFT_36_TM=60.556
PRIMER_RIGHT_36_TM=59.505
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=990
PRIMER_PAIR_37_PENALTY=2.050849
PRIMER_LEFT_37_PENALTY=1.556038
PRIMER_RIGHT_37_PENALTY=0.494811
PRIMER_LEFT_37_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_37_SEQUENCE=TGGAGGAGTGGATGAGGTTT
PRIMER_LEFT_37=755,21
PRIMER_RIGHT_37=1745,20
PRIMER_LEFT_37_TM=60.556
PRIMER_RIGHT_37_TM=59.505
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=991
PRIMER_PAIR_38_PENALTY=2.080648
PRIMER_LEFT_38_PENALTY=1.132718
PRIMER_RIGHT_38_PENALTY=0.947930
PRIMER_LEFT_38_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_38_SEQUENCE=AGATGGAGGAGTGGATGAGG
PRIMER_LEFT_38=757,21
PRIMER_RIGHT_38=1748,20
PRIMER_LEFT_38_TM=59.867
PRIMER_RIGHT_38_TM=59.052
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=992
PRIMER_PAIR_39_PENALTY=2.082579
PRIMER_LEFT_39_PENALTY=1.132718
PRIMER_RIGHT_39_PENALTY=0.949861
PRIMER_LEFT_39_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_39_SEQUENCE=GTGGATGAGGTTTGGGGATT
PRIMER_LEFT_39=757,21
PRIMER_RIGHT_39=1738,20
PRIMER_LEFT_39_TM=59.867
PRIMER_RIGHT_39_TM=60.950
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=982
PRIMER_PAIR_40_PENALTY=2.145729
PRIMER_LEFT_40_PENALTY=2.141487
PRIMER_RIGHT_40_PENALTY=0.004242
PRIMER_LEFT_40_SEQUENCE=CCAAATTGATTCGACATTCC
PRIMER_RIGHT_40_SEQUENCE=GGATGAGGTTTGGGGATTTT
PRIMER_LEFT_40=870,20
PRIMER_RIGHT_40=1736,20
PRIMER_LEFT_40_TM=57.859
PRIMER_RIGHT_40_TM=59.996
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=7.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=867
PRIMER_PAIR_41_PENALTY=2.149058
PRIMER_LEFT_41_PENALTY=2.141487
PRIMER_RIGHT_41_PENALTY=0.007570
PRIMER_LEFT_41_SEQUENCE=CCAAATTGATTCGACATTCC
PRIMER_RIGHT_41_SEQUENCE=GGAGATGGAGGAGTGGATGA
PRIMER_LEFT_41=870,20
PRIMER_RIGHT_41=1750,20
PRIMER_LEFT_41_TM=57.859
PRIMER_RIGHT_41_TM=60.008
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=6.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=881
PRIMER_PAIR_42_PENALTY=2.157539
PRIMER_LEFT_42_PENALTY=2.141487
PRIMER_RIGHT_42_PENALTY=0.016052
PRIMER_LEFT_42_SEQUENCE=CCAAATTGATTCGACATTCC
PRIMER_RIGHT_42_SEQUENCE=CGATGACACGACGAGAAAGA
PRIMER_LEFT_42=870,20
PRIMER_RIGHT_42=1677,20
PRIMER_LEFT_42_TM=57.859
PRIMER_RIGHT_42_TM=59.984
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.1000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=808
PRIMER_PAIR_43_PENALTY=2.169793
PRIMER_LEFT_43_PENALTY=1.556038
PRIMER_RIGHT_43_PENALTY=0.613756
PRIMER_LEFT_43_SEQUENCE=CTTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_43_SEQUENCE=ATTTTGGAATCGGAAGGGAA
PRIMER_LEFT_43=755,21
PRIMER_RIGHT_43=1721,20
PRIMER_LEFT_43_TM=60.556
PRIMER_RIGHT_43_TM=60.614
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=967
PRIMER_PAIR_44_PENALTY=2.188571
PRIMER_LEFT_44_PENALTY=1.132718
PRIMER_RIGHT_44_PENALTY=1.055853
PRIMER_LEFT_44_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_44_SEQUENCE=AGATGGAGGAGTGGATGAGGT
PRIMER_LEFT_44=757,21
PRIMER_RIGHT_44=1748,21
PRIMER_LEFT_44_TM=59.867
PRIMER_RIGHT_44_TM=59.944
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=992
PRIMER_PAIR_45_PENALTY=2.231643
PRIMER_LEFT_45_PENALTY=2.141487
PRIMER_RIGHT_45_PENALTY=0.090155
PRIMER_LEFT_45_SEQUENCE=CCAAATTGATTCGACATTCC
PRIMER_RIGHT_45_SEQUENCE=TTTCTTCGAAATCACACCCC
PRIMER_LEFT_45=870,20
PRIMER_RIGHT_45=1595,20
PRIMER_LEFT_45_TM=57.859
PRIMER_RIGHT_45_TM=59.910
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=10.6000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=726
PRIMER_PAIR_46_PENALTY=2.233243
PRIMER_LEFT_46_PENALTY=1.132718
PRIMER_RIGHT_46_PENALTY=1.100525
PRIMER_LEFT_46_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_46_SEQUENCE=GATGGAGGAGTGGATGAGGT
PRIMER_LEFT_46=757,21
PRIMER_RIGHT_46=1747,20
PRIMER_LEFT_46_TM=59.867
PRIMER_RIGHT_46_TM=58.899
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=991
PRIMER_PAIR_47_PENALTY=2.260268
PRIMER_LEFT_47_PENALTY=1.132718
PRIMER_RIGHT_47_PENALTY=1.127550
PRIMER_LEFT_47_SEQUENCE=TGGTCTTTTGCTCGTCTCATT
PRIMER_RIGHT_47_SEQUENCE=GATTTTGGAATCGGAAGGGA
PRIMER_LEFT_47=757,21
PRIMER_RIGHT_47=1722,20
PRIMER_LEFT_47_TM=59.867
PRIMER_RIGHT_47_TM=61.128
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=966
PRIMER_PAIR_48_PENALTY=2.261361
PRIMER_LEFT_48_PENALTY=0.434141
PRIMER_RIGHT_48_PENALTY=1.827221
PRIMER_LEFT_48_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_48_SEQUENCE=GGGAGATGGAGGAGTGGATG
PRIMER_LEFT_48=756,20
PRIMER_RIGHT_48=1751,20
PRIMER_LEFT_48_TM=59.566
PRIMER_RIGHT_48_TM=61.827
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=60.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=996
PRIMER_PAIR_49_PENALTY=2.275062
PRIMER_LEFT_49_PENALTY=0.434141
PRIMER_RIGHT_49_PENALTY=1.840921
PRIMER_LEFT_49_SEQUENCE=TTGGTCTTTTGCTCGTCTCA
PRIMER_RIGHT_49_SEQUENCE=TTTCTTCGAAATCACACCCCT
PRIMER_LEFT_49=756,20
PRIMER_RIGHT_49=1595,21
PRIMER_LEFT_49_TM=59.566
PRIMER_RIGHT_49_TM=60.841
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=10.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=840
=
SEQUENCE_ID=MAGIC.433_3_13153159_13153197_oy-0_can-0_2081_6470_TRUE
SEQUENCE_TARGET=950,138
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAGAGTTTCGGCTANTGAAGCGTTGNTAGCNTCTTCTCCAGACNTTGCTNCTCACTTCGGAAATGTANTANATAGAANAAATNTNANNACTCAAGAATCCTNCGTCCCCACAGANGNCGCCAAAATNTGGATGTCGGATTCACACACTCAANTATTTATTTNTTTANTGAGAAGGATTATTCTTGAGGACAAATGTACTAGAAAATANANNAATGATTATAGAGAAAACTATGAGTTTTGTAAGAAAATNNNNNANAATCCANTATATAAAANCANATGTTCAAAAACAAATATGCCTAAGAAAAGGGAAATTTGGTCGTTTGTACCACATTCACNAAAAAAANCTANCCGTTTGTANCACATTCATGGAGACATNCAAGTTTNNTGGACCAATATGCCCTTGACNNAGACATGTGTCAGAGNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_13170982_13171184_can-0_mt-0_14133_314689_TRUE
SEQUENCE_TARGET=950,302
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTGTNAAANTANTAANNNNNNNNNNNNNNNNNNNNNNNNAGTTAGGCCGATNANTCNAACNATATAGCAAGTAANNANANTAAANNGTAATTTNTAACNNGTGAGTGANTTNGTTTGAGNAACCANAANGGNCTNAGNTTAGGGTTNGCATCTCNGGGCTCANNCTCTTCNTNNGANGATTTGNNNNNNNNNNNNNNNNNCANNCCNANATCTAGAAGAAGNNNNNNNNATGACTGGAGGAGGGGGACCCATTGATCGNGGTNGGGGAGATGATCGAGGAGAACNATCAGTTCNGGCTNTNNTCTTATCTCTCTTAGGANAGGAACTGCTGCGNGTTGNTCTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNCNGANGTGAGTCACGNNNNNNNNNNNNNNNNNNNCGAGGTGACCNGCNGCCATGACCTTNANGNTGAGGTGTNGGTCTNAANGCACNNGCAGATNTNNCCCCTTCGNCGCTACCATCNTCGACNAGNANNGTTCTACTGTANTCNTTCNTGTCGACAGCNGACTNNAGNAATGANTTGTTCNCCTCACANGCCTNTNTCATNGGCTTGGANAACNNNNNNNNNNNNGAAGCGATAGCCCTNNCAGAAGTTCCGTGAGGTANAGNTGGAGNTGGTGGAAGTTGNGTNGNNNNCCGGNTGGNCTCATTNTGNNNTTATNATTNGCCTNAANGAACCGNTCGANNGTGAAAGAGTTCNAGAGGGTCTCNCNNTCGATNACNATCTTNANGAANNNNNNNNNNNNNNNNNTNGGGAGAGTTGGAGNAATGAAAGCTNNNAANTNNAAATNATAAAATNNATTANNNNNNNNNNNNNNNNNNNCNAAAAGNCATATNTNACCTAANTTCNACCAGGTTTNNNAGACCACTTCGATGGAAGCANNNNNNNNNNNTTTCNAATCGAGGCAGGGGTTTTNTTAATCANNAACCAAGGATGGNGCCAANTCTCATCCTTGTTCGNNANATTCGNGATGANATTCNTGTTGGNGTTNGGNTACNCTGAAAANTACCNNGTTTTNTTCGNCGAACTCCTNANACTGAGAAGTTNGTTCAATTCNGGAACCCCGATGANANAANCGNNNNNNGNNNCGATGGTAATAATACNCANGATGGTTNGGAGGAGGTTANGGGTNANTTGAGGTANGGNGATTTCNAGNGCNGCCAAATATCNAACNANNGCTTNGGGAAGAGGNAAGGTCAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_14220064_14220081_hi-0_sf-2_7950_265576_TRUE
SEQUENCE_TARGET=950,117
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATNGNNGAACCNNNNNNNTNNTTTNTTNANNNNCNAGAAATGTATCTTTGCCACATATGAGTATTTTACCTTATTTCGGTTNGNNCTAAGTTGTTNTCTTAATTGNNCATTCTACCTAACCANGANATTGGTCAGTNNTACTNAAANTGTATNGAANANTTTAATCANACANNANNTCNTTTANTAGTGANCAATGGTTTNAAGGTTCTCANACTTNAAAAAATANCCNAANNNCTTNATAAAANTANNNAACCCCTTAGNCCTTAGCTNGACTTNATCTTACAAAGTTCAAAAATCCGAGAGCAGNTTNTTACANCTGACTATGTATGTNTAGGCCACAAAATAAGTCTTANNNNTNNTTTTGGGATCCCTCNTAACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTNACCAAGNAAAAANAGGTGTTCAAATAGAGTATCTTTATTAATGTTCCAAACCTTTTTACATTGAAAANCAAGANTTTTTTTCTNNNNTTTTTCTNTTTTTGGTCAAGNNAAACANGAANNTAAATGAACTTNTTACATTGAAAAANACAAGAAGGAGATAAATGAAANCAGTATAACACGGGTGATGAGCTTNANATGTATGGTCATCATCAAAGGAGCAACCGTATAGCCTTGGTGTAGTGTGTTAGTGCCCAAAGAGTGAAAGAGTTTCTAAAGGTTGGAAAATTTAGCATCACATTCATTTTGTAAACACCCCTTATTTCCTACAANATNGAANNTACAAATGAGCAATAATATCACAGTNAANTTGAAANTCTTAAATAAAATTAGGGTAANAAGAANCANACCTGNTGTGGAAAATCACCGTTATCCATTTGTGAATTGATTAATACTTTTGCAGCNCGGTGAACCGGTAAAGGATCTCTATCCATCTGACCACCCATTATTAGAACCATCAANGCTTGTCCTGTATTCACCACATNGGTCTTGTTCC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_2780200_2780227_zu-0_po-0_189637_36065_TRUE
SEQUENCE_TARGET=950,127
SEQUENCE_TEMPLATE=CCAAAAGGATTGAACNNAAANAAAANATATTACNAAAAGGAAAAGTTATTTACAATCTTNTTTCNTTTTATCACCTTATATNCTNTTCTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGATAGTCAATNATACAGTTAGGTGATCACGTAAACAATCATTAAGAGTNTNCTAGCTAGTGATTATTGATGATGNTGTGATTTGCATGGGGACACCATACCTNGTNTTTTGGACATTGTTGATANAAAGATCAAATGAAATTGGTGATGAAACACCAANGGAAAAGTGTNACACGNTTATATTCTCTNNNNNNCTACNGGTCTACTCAANATTTTNTTTNAGCTATCAATCGCAAGGCTTTGNATTGATGCTAAAATTAGTGGTTTTCATAAGTAAATTTAATTAATTATAATNTTATTANTNTTGATAGTATGTAACTAAANAATCGTGAAAGTAATGTACCAACTNAAAAAAATATAAAGACCCNTGNCATCNTTCTCGANCGACTTANAATCNCGCNCTCAAAACTTCTTNNAAAAGTTCAAAGNAAANNCNCTNANACTNNNNNNNNNNNNNNNNNNNNNNNTTCATAAGTCAAANAACAAAGGTGTCNATTGTCNAANNNANAAAANAAANNAACAACAACAAAGNTATNTCCGCAAGATTCAGAAAGATTNNATATCTAGTACCAATGATAGTCGGNNTCNTTANANTGTGACCCAAATGTTGTCACCGCACCNTNATNNNAGNCANCATCTGAANCGTCATCTGAGTCATTGGGGCANCTCAAGTCTTTGATNCTCTCAAAACATCGTTTCTTGAGATCCCCAACAAGGTTGATGTTGAAACACTGGCGTAAATCAAGGTGTTCNAGGTGAGGACAACCATCAAGAATGGCGTTCAAGCCCTTNTTGGTTAGCCCGTTCCCTAAAAGCTGGAGGTGGCGTAGTTCAGGCATGCTTTCTGCAATTGCTTTGGCATTATCATCAAACCCACTATTTGAAAACATGATTTCAGGGGCTCGGTTTAGCTTCAGTGTCTTCAGATNCGGACAAGACCGGCCTATATCTCTCAGAGACTCTCCTGAAAACAAGCAGTATGAAACCTCAAGGTATTCAAGCAACGGTACTTTCGAAACTGCTTTGGCAACTCCCTCATCTGTTATTGGAAAGCACCTTACAAGTCCAAGGCTTTTNAGATTACTTGACCTGCAAATANTTGTTACTAAAAAAGTCAAAAACATAACCTTATTGAGAACACTTCGTTGACAAAACCACTAAGCAAACCANGGTGCNTTATCAGANATTCAGTAGAAGGANATAGAANAGATAAAAATAAATCTAGTTTAAAAAGAGTGACACAAAATCNTCCAATCACTTATANCTNCANAATTAGCAATCACTCNAACCCTTGGATTAGCACCGATCACTCTTAGCCCNNNNNNTAGCAAACACTCTAACCCTAGTATTAGCANCNACCAAATTNNNTTTAACATGGAAAGAGAGCTAGNGAGAAACAGAACCTGTCNGCTATGTACNTGATGAGATCATTAGTACCNTAGTACCAAATCTCGATCTCAANCAAACCACCNTGGCTACGATCAACTGCGNGACGGCACATTGACTCGAGGTCGTACTTGAAGGCTNCCCTATCATTGCGGTTATCTATGTCGATCTTCCGCCACATCGAGGGGTCTTTGCAGACTCGATGCCACGGTTTGCACACTTTTTGAGCGTTTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_13507089_13507604_oy-0_ct-1_132367_51813_TRUE
SEQUENCE_TARGET=950,615
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNTGTTGATGATATNACAAATCTGATANAAGAGTGCTTNTTGCANGGGANNAAAGNANCTTTGGTCNAGGTTAGCTAGTTTTAATTAAGTTTANGACTAAGCTAATCATCACNTGTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTAGGGNNTACCCNTACGATNGTTGCCNATNTCATAGCATTTGTNAATNCGCTATGTTATATGTATGATAANAGATNCGCGNNAATAATGTCAANATTTCTTGTAGTGAAANGAAGCTGAANAAGAANNTNCNTNANNAACTNAACAAGAAGCTGCTGCNCANGAAGCTGAACNNNNNNNNNNNNANNAAGCTACAAAAGAANTTGACNAATATTTACACATTTTNNANANATTAAACATTNNATNAAGCTAACTNAANATATTATTGTNTTGGANGTGNAGANGAACAAAGGTGAGACAAATGCTGAGAACNAAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGAAACAATGATNANCTTGAGGTGAGTTGTNTATGTTTTNGAGCAAACTGATTTNNNAATGATTTATACACTACAANAAATNAGGACATTACTAGCACANGNAAAATGCTACTAAANNACTTTCGTANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4523766_4523983_zu-0_kn-0_20485_28227_TRUE
SEQUENCE_TARGET=950,317
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_8561977_8562148_wil-2_ct-1_48223_4975_TRUE
SEQUENCE_TARGET=950,271
SEQUENCE_TEMPLATE=TAACNNGTTTGACATATATATTTCAAAANCCACTAACNAAAATATTAATAAATACTAGTTTTTCGAAGNNAANAAAAAAACGTTTGCATTATTTAGTCTTGAAACAACNAATTNAAAAGGAAAGGTTGTCGGTGAACAAATTTGAAGGCCACTNGAATAATGCCTTNTATTTTTGTCAGAAGNTTACATTGNCTAGGTACTTGTTTAACTTAATTCNTACAATTTCTGATACCAATTGTGTAATATCACTGAGTTAGTTGTTNNTGTTGTTTCATTANCCAAAAAACAAAAGTTCTACACGTTGGCATNCCTTTCTTTTGGTCATATATGAGTTTGATAACAATATATAAACTTCAAAATCAGGATTTTCTTTGCTAAATCGAAAGAGTTTCGTANAANATTATTGATTGTTTTAAGTTTAATTAAGTAACTTGTAGGTTTAATTCAAAAACTTGTAGGTATCTCNGTGATATTANTGGTTTATGAAANNNNNTATTAANGCTTGTNTCGANTTTGTNTTNGGCCGGCCTTTCTTANCAGAATTGAACCTCAGANAGAAACTGTAATAAACCAAAGATACTGGACATGTNTATGTCGGCCTTTGGNGTCGTCTGTAACTAAAAANAAACTGGATANTTATCCNNGATATTACTAATCNTNTGAAATCTTNTTGTAATTATCTATAGATTNTTAGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTTGAATGTAAAATCCANNNTTGGATAGTATCCAATCNGTAGCCGNTATNNTCCCAACTTTTAATCTNAAATATGCATGATTANAANTATCAAAATATGATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4388465_4388585_ler-0_zu-0_13536_2140_TRUE
SEQUENCE_TARGET=950,220
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACTNCAAACTCTCCCTATCACTCTTCANTAATTCGTTCTCACTTNATCATACTTCACCAAANTTACTCGATCACTTCCAAGCTCATCCTCAAGCATTCAATNGGTNNCAAGCTCAAAATTTCATCACCNAAGAAANNNATTCCCTCCCCCTTTGATNCCCNTTGGAGAGTTGTTTTTAANTNCTTTNTTGAAACTCACCACCATGNATTCAAGAAGGAAGAGTTCGTCTNGNGCAAGAANNNNNNNNNNNGATCCTGANNAAGTNNNNNNNNNNNNNNTGAGTGNCATGACTGAACGCCAAATTCGCCAAGCTCGTCGTGNCAAAGATCAAGNTTGATTCAAGGAGGANGCTCAAGNCGNTCGACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_1460008_1460271_rsch-4_po-0_68737_79772_TRUE
SEQUENCE_TARGET=950,363
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5514037_5514069_oy-0_tsu-0_16753_19042_TRUE
SEQUENCE_TARGET=950,132
SEQUENCE_TEMPLATE=GACCCATCCCGCCTTCTGCGTCTGNNGTACATCTACCNGCCTTTTTGATTCAGAANGCTGCTCGCTTTCGGTTTCTNCTCCCNGGAGGTTGTCTCCGAGAACCCTAGCTGAAGATTAAGTACAAAATAGAGAAGCAATAGTTTCTTGTTANAAAGGTTTTTGGCGTCACACCAACTTAACNTGAAACAACNGATAAATAAANAAATAGANCTTATNACATGTTCTGTTTTCTTACAAANCTTATGGTGATTACAAGACCGGGCCAANAACCATCNTTAGTCTCACTCTAGNNNNNNNNTAACTCAACCTTATANACAAGCANATCTNNNTTAAACNCANTNGANAAAGAANAGGTAAGCCACATAGCNTATAGNNNGANANTCANTATGAACGTAAANANAGANTCAAGNAACAATATNACCANNAACCAGTGATTGTTNNAANGTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTNTTTCTTTNTCGTAANATGNCCNTNATGTTTTTGGCAAAANNNTTTTNCANNTATTGTTGTAATGTTTTCACAANNNTTCTTGTAANTTGTCNTNNTTTTCGAGTNTCNTCTTGCTTNTATNNNNNNNNNNNNNNTGAAATTAACAACTNNNAAAAATANTGTAAAATCAATGTGATNAAAAATCAAATTTATANCNAAAAATAGTTCTTAGCNAAATACANATCAACTATAGAACATTATTCATANTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGTCTANTGATANNTACCTCCGCAATAAGATCCAGGGNCTAAGANGTNGCCGCATGCATGAGAGAGTTTGGAGAGNTTATAAAAGTCAAAGTTTTGCGAAAACGTTTTTGTAACGACTTGGCATATGCATTTCATCTTGTTCANTNTTATAGTATCACAACAAACTTTCAATGGANAANCCCAAGGAACGCCACGCGNGAGAGAATCNCCACAATAATCATATTCTTCAGAAATCTCTGTCCAAGTACANGGAGCATTAACTTGGGAAGATGTTACTCTAGTCACCAACAGTGTTANCAAAAACATAGAGACTNTANTNTAAGCTNTCACCATTTNTTCTANTTTCTTTTTCCTTATATGGGCGTATTTGTCTTTAGAATCTTCATTGATGCTTTGANATATACTGCNAAACCCAATGTAACGAATATCAAATCTCACAAGTATAAAGGAAAAAGTATGGTCAATCACAAGAGAATATAAATTTACTCATTTACCATTTTGATAAAGTCAATAANACCATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAATAATAAAAATAGAACATATTNAAANGNNAAAAAACTTCACATTTNAANAAANCACTAAACGAATGAACGAAGAATGTTGAACACTATATATNGTGATAGTCAAGCAAGGTTACNATATNGACCAATGACCATCAATANNNNNNNNNTNANGTAGGGGANTNACNCGCANCTTGCGCGGCCAAGNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_1379310_1379664_rsch-4_oy-0_26428_149110_TRUE
SEQUENCE_TARGET=950,454
SEQUENCE_TEMPLATE=ACCAGTAAGACAAACCAATAGCCANGGCGGCTCCATTGCTTCCAAGACCAAACGCGTAAACCAGAATCAAACAAACTGGTATATGGAACAAAAGGGTGGTTATGGCACAGTAGAGAAGAGGAAGAACCAAACCCTGAGTCTGGAGAAACCGAGTCAAAGGTTGTTGCACCGCTTGAGCTAACAATGCCGGTATAAGACAAACCGCGTAAGAACCAGCTACCTTGGAGATGTCAGGATCTTGTCCAAGTGAAANNAAGAGTTTGTCCATGTAAAACCAGAGAATCGATATGAGAACAACTATNGGTACGTTTGAGACTATTGCAGAGAAAGTGTAAGTTCCGATTTTCGCGTATTGTTTTGCTCCATAAGCTTGGCCACATAGAGTTTCAAGTGCACCCNCTAAACCAAACTGCAAANAAANATTCAAGACTTTGAGATTCGAATGNATCTTGAAACTGAGGATTNATAACANNNNNNNNNTAGTTACCATGACACTGAAGCCGGANACGTTTGTAAAAGAAGTGGCAAGAGCAACGCCTGAGAGCTGGAGTTCGCTGCGGTGGCCGGCGACCATNACTGAGATAACGGGTAATAGATATTGAGCAACAGTCACGGTGGCCATAGGCAGCGCCATGCGGCTGAGGTTCTTGAGCTCCGCNGTGAAATTCATCCTCCATGTNGGCGTCGGCTTAAGACTTCCGGTGATTATCTGATTGTCTCCCAATAAAANTNGATCTTCCATTATCNNNNNNNNNNNNNNNNNNNNNNGTCNACGATNNTTTCTTNACCTTGGAACGTGTGAGATGAGAATGCACGACNNNNNNNAACANAAAACAGAGNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCTTTAATATAGATAAATTCACTAAAAATNATGTATAAATAATTTAATTTAATGGACCATAAATTCAATATTGGATTTTTCCACGGCTCTCTAAAAAGACTAAAATTCAAACAAAAATAACATTGAATNTTCCATTAAACATATTATTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTNAATAGTTTATAAAACNTATATAACATATATCCTCTATCACTAATTTATTTTNTTAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.587298
PRIMER_LEFT_0_PENALTY=0.324275
PRIMER_RIGHT_0_PENALTY=2.263023
PRIMER_LEFT_0_SEQUENCE=CCTTGGAACGTGTGAGATGA
PRIMER_RIGHT_0_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_0=787,20
PRIMER_RIGHT_0=1786,22
PRIMER_LEFT_0_TM=59.676
PRIMER_RIGHT_0_TM=59.737
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=1000
PRIMER_PAIR_1_PENALTY=2.687549
PRIMER_LEFT_1_PENALTY=0.324275
PRIMER_RIGHT_1_PENALTY=2.363274
PRIMER_LEFT_1_SEQUENCE=CCTTGGAACGTGTGAGATGA
PRIMER_RIGHT_1_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_1=787,20
PRIMER_RIGHT_1=1786,20
PRIMER_LEFT_1_TM=59.676
PRIMER_RIGHT_1_TM=57.637
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=1000
PRIMER_PAIR_2_PENALTY=2.869348
PRIMER_LEFT_2_PENALTY=0.324275
PRIMER_RIGHT_2_PENALTY=2.545073
PRIMER_LEFT_2_SEQUENCE=CCTTGGAACGTGTGAGATGA
PRIMER_RIGHT_2_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_2=787,20
PRIMER_RIGHT_2=1785,21
PRIMER_LEFT_2_TM=59.676
PRIMER_RIGHT_2_TM=58.455
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=999
PRIMER_PAIR_3_PENALTY=3.006914
PRIMER_LEFT_3_PENALTY=1.104440
PRIMER_RIGHT_3_PENALTY=1.902474
PRIMER_LEFT_3_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_3_SEQUENCE=TCTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_3=797,20
PRIMER_RIGHT_3=1790,21
PRIMER_LEFT_3_TM=58.896
PRIMER_RIGHT_3_TM=59.098
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=994
PRIMER_PAIR_4_PENALTY=3.048986
PRIMER_LEFT_4_PENALTY=1.146512
PRIMER_RIGHT_4_PENALTY=1.902474
PRIMER_LEFT_4_SEQUENCE=TGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_4_SEQUENCE=TCTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_4=797,21
PRIMER_RIGHT_4=1790,21
PRIMER_LEFT_4_TM=59.853
PRIMER_RIGHT_4_TM=59.098
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=994
PRIMER_PAIR_5_PENALTY=3.048986
PRIMER_LEFT_5_PENALTY=1.146512
PRIMER_RIGHT_5_PENALTY=1.902474
PRIMER_LEFT_5_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_5_SEQUENCE=TCTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_5=796,21
PRIMER_RIGHT_5=1790,21
PRIMER_LEFT_5_TM=59.853
PRIMER_RIGHT_5_TM=59.098
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=995
PRIMER_PAIR_6_PENALTY=3.212203
PRIMER_LEFT_6_PENALTY=1.104440
PRIMER_RIGHT_6_PENALTY=2.107763
PRIMER_LEFT_6_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_6_SEQUENCE=GTCTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_6=797,20
PRIMER_RIGHT_6=1791,22
PRIMER_LEFT_6_TM=58.896
PRIMER_RIGHT_6_TM=59.892
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=995
PRIMER_PAIR_7_PENALTY=3.254275
PRIMER_LEFT_7_PENALTY=1.146512
PRIMER_RIGHT_7_PENALTY=2.107763
PRIMER_LEFT_7_SEQUENCE=TGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_7_SEQUENCE=GTCTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_7=797,21
PRIMER_RIGHT_7=1791,22
PRIMER_LEFT_7_TM=59.853
PRIMER_RIGHT_7_TM=59.892
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.8000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=995
PRIMER_PAIR_8_PENALTY=3.254275
PRIMER_LEFT_8_PENALTY=1.146512
PRIMER_RIGHT_8_PENALTY=2.107763
PRIMER_LEFT_8_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_8_SEQUENCE=GTCTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_8=796,21
PRIMER_RIGHT_8=1791,22
PRIMER_LEFT_8_TM=59.853
PRIMER_RIGHT_8_TM=59.892
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=996
PRIMER_PAIR_9_PENALTY=3.291878
PRIMER_LEFT_9_PENALTY=0.324275
PRIMER_RIGHT_9_PENALTY=2.967603
PRIMER_LEFT_9_SEQUENCE=CCTTGGAACGTGTGAGATGA
PRIMER_RIGHT_9_SEQUENCE=TAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_9=787,20
PRIMER_RIGHT_9=1784,20
PRIMER_LEFT_9_TM=59.676
PRIMER_RIGHT_9_TM=57.032
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=998
PRIMER_PAIR_10_PENALTY=3.367463
PRIMER_LEFT_10_PENALTY=1.104440
PRIMER_RIGHT_10_PENALTY=2.263023
PRIMER_LEFT_10_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_10_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_10=797,20
PRIMER_RIGHT_10=1786,22
PRIMER_LEFT_10_TM=58.896
PRIMER_RIGHT_10_TM=59.737
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=990
PRIMER_PAIR_11_PENALTY=3.404126
PRIMER_LEFT_11_PENALTY=1.104440
PRIMER_RIGHT_11_PENALTY=2.299686
PRIMER_LEFT_11_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_11_SEQUENCE=TCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_11=797,20
PRIMER_RIGHT_11=1790,20
PRIMER_LEFT_11_TM=58.896
PRIMER_RIGHT_11_TM=57.700
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=994
PRIMER_PAIR_12_PENALTY=3.409535
PRIMER_LEFT_12_PENALTY=1.146512
PRIMER_RIGHT_12_PENALTY=2.263023
PRIMER_LEFT_12_SEQUENCE=TGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_12_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_12=797,21
PRIMER_RIGHT_12=1786,22
PRIMER_LEFT_12_TM=59.853
PRIMER_RIGHT_12_TM=59.737
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.8000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=990
PRIMER_PAIR_13_PENALTY=3.409535
PRIMER_LEFT_13_PENALTY=1.146512
PRIMER_RIGHT_13_PENALTY=2.263023
PRIMER_LEFT_13_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_13_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_13=796,21
PRIMER_RIGHT_13=1786,22
PRIMER_LEFT_13_TM=59.853
PRIMER_RIGHT_13_TM=59.737
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=991
PRIMER_PAIR_14_PENALTY=3.446198
PRIMER_LEFT_14_PENALTY=1.146512
PRIMER_RIGHT_14_PENALTY=2.299686
PRIMER_LEFT_14_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_14_SEQUENCE=TCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_14=796,21
PRIMER_RIGHT_14=1790,20
PRIMER_LEFT_14_TM=59.853
PRIMER_RIGHT_14_TM=57.700
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=995
PRIMER_PAIR_15_PENALTY=3.467715
PRIMER_LEFT_15_PENALTY=1.104440
PRIMER_RIGHT_15_PENALTY=2.363274
PRIMER_LEFT_15_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_15_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_15=797,20
PRIMER_RIGHT_15=1786,20
PRIMER_LEFT_15_TM=58.896
PRIMER_RIGHT_15_TM=57.637
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=990
PRIMER_PAIR_16_PENALTY=3.509786
PRIMER_LEFT_16_PENALTY=1.146512
PRIMER_RIGHT_16_PENALTY=2.363274
PRIMER_LEFT_16_SEQUENCE=TGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_16_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_16=797,21
PRIMER_RIGHT_16=1786,20
PRIMER_LEFT_16_TM=59.853
PRIMER_RIGHT_16_TM=57.637
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.8000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=990
PRIMER_PAIR_17_PENALTY=3.509786
PRIMER_LEFT_17_PENALTY=1.146512
PRIMER_RIGHT_17_PENALTY=2.363274
PRIMER_LEFT_17_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_17_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_17=796,21
PRIMER_RIGHT_17=1786,20
PRIMER_LEFT_17_TM=59.853
PRIMER_RIGHT_17_TM=57.637
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=991
PRIMER_PAIR_18_PENALTY=3.513795
PRIMER_LEFT_18_PENALTY=1.104440
PRIMER_RIGHT_18_PENALTY=2.409355
PRIMER_LEFT_18_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_18_SEQUENCE=GTCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_18=797,20
PRIMER_RIGHT_18=1791,21
PRIMER_LEFT_18_TM=58.896
PRIMER_RIGHT_18_TM=58.591
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=995
PRIMER_PAIR_19_PENALTY=3.555867
PRIMER_LEFT_19_PENALTY=1.146512
PRIMER_RIGHT_19_PENALTY=2.409355
PRIMER_LEFT_19_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_19_SEQUENCE=GTCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_19=796,21
PRIMER_RIGHT_19=1791,21
PRIMER_LEFT_19_TM=59.853
PRIMER_RIGHT_19_TM=58.591
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=996
PRIMER_PAIR_20_PENALTY=3.561323
PRIMER_LEFT_20_PENALTY=1.104440
PRIMER_RIGHT_20_PENALTY=2.456883
PRIMER_LEFT_20_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_20_SEQUENCE=AGTCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_20=797,20
PRIMER_RIGHT_20=1792,22
PRIMER_LEFT_20_TM=58.896
PRIMER_RIGHT_20_TM=59.543
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.4000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=996
PRIMER_PAIR_21_PENALTY=3.603395
PRIMER_LEFT_21_PENALTY=1.146512
PRIMER_RIGHT_21_PENALTY=2.456883
PRIMER_LEFT_21_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_21_SEQUENCE=AGTCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_21=796,21
PRIMER_RIGHT_21=1792,22
PRIMER_LEFT_21_TM=59.853
PRIMER_RIGHT_21_TM=59.543
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=997
PRIMER_PAIR_22_PENALTY=3.649513
PRIMER_LEFT_22_PENALTY=1.104440
PRIMER_RIGHT_22_PENALTY=2.545073
PRIMER_LEFT_22_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_22_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_22=797,20
PRIMER_RIGHT_22=1785,21
PRIMER_LEFT_22_TM=58.896
PRIMER_RIGHT_22_TM=58.455
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.4000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=989
PRIMER_PAIR_23_PENALTY=3.691585
PRIMER_LEFT_23_PENALTY=1.146512
PRIMER_RIGHT_23_PENALTY=2.545073
PRIMER_LEFT_23_SEQUENCE=TGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_23_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_23=797,21
PRIMER_RIGHT_23=1785,21
PRIMER_LEFT_23_TM=59.853
PRIMER_RIGHT_23_TM=58.455
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.8000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=989
PRIMER_PAIR_24_PENALTY=3.691585
PRIMER_LEFT_24_PENALTY=1.146512
PRIMER_RIGHT_24_PENALTY=2.545073
PRIMER_LEFT_24_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_24_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_24=796,21
PRIMER_RIGHT_24=1785,21
PRIMER_LEFT_24_TM=59.853
PRIMER_RIGHT_24_TM=58.455
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=990
PRIMER_PAIR_25_PENALTY=3.854668
PRIMER_LEFT_25_PENALTY=1.104440
PRIMER_RIGHT_25_PENALTY=2.750228
PRIMER_LEFT_25_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_25_SEQUENCE=CTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_25=797,20
PRIMER_RIGHT_25=1789,20
PRIMER_LEFT_25_TM=58.896
PRIMER_RIGHT_25_TM=57.250
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=993
PRIMER_PAIR_26_PENALTY=3.896740
PRIMER_LEFT_26_PENALTY=1.146512
PRIMER_RIGHT_26_PENALTY=2.750228
PRIMER_LEFT_26_SEQUENCE=TGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_26_SEQUENCE=CTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_26=797,21
PRIMER_RIGHT_26=1789,20
PRIMER_LEFT_26_TM=59.853
PRIMER_RIGHT_26_TM=57.250
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.8000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=993
PRIMER_PAIR_27_PENALTY=3.896740
PRIMER_LEFT_27_PENALTY=1.146512
PRIMER_RIGHT_27_PENALTY=2.750228
PRIMER_LEFT_27_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_27_SEQUENCE=CTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_27=796,21
PRIMER_RIGHT_27=1789,20
PRIMER_LEFT_27_TM=59.853
PRIMER_RIGHT_27_TM=57.250
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=994
PRIMER_PAIR_28_PENALTY=3.958394
PRIMER_LEFT_28_PENALTY=1.695371
PRIMER_RIGHT_28_PENALTY=2.263023
PRIMER_LEFT_28_SEQUENCE=CCTTGGAACGTGTGAGATGAG
PRIMER_RIGHT_28_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_28=787,21
PRIMER_RIGHT_28=1786,22
PRIMER_LEFT_28_TM=60.695
PRIMER_RIGHT_28_TM=59.737
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=1000
PRIMER_PAIR_29_PENALTY=3.978715
PRIMER_LEFT_29_PENALTY=1.433642
PRIMER_RIGHT_29_PENALTY=2.545073
PRIMER_LEFT_29_SEQUENCE=ACCTTGGAACGTGTGAGATG
PRIMER_RIGHT_29_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_29=786,20
PRIMER_RIGHT_29=1785,21
PRIMER_LEFT_29_TM=58.566
PRIMER_RIGHT_29_TM=58.455
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=1000
PRIMER_PAIR_30_PENALTY=3.985506
PRIMER_LEFT_30_PENALTY=1.722483
PRIMER_RIGHT_30_PENALTY=2.263023
PRIMER_LEFT_30_SEQUENCE=TTGGAACGTGTGAGATGAGAA
PRIMER_RIGHT_30_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_30=789,21
PRIMER_RIGHT_30=1786,22
PRIMER_LEFT_30_TM=59.278
PRIMER_RIGHT_30_TM=59.737
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=998
PRIMER_PAIR_31_PENALTY=4.058646
PRIMER_LEFT_31_PENALTY=1.695371
PRIMER_RIGHT_31_PENALTY=2.363274
PRIMER_LEFT_31_SEQUENCE=CCTTGGAACGTGTGAGATGAG
PRIMER_RIGHT_31_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_31=787,21
PRIMER_RIGHT_31=1786,20
PRIMER_LEFT_31_TM=60.695
PRIMER_RIGHT_31_TM=57.637
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=1000
PRIMER_PAIR_32_PENALTY=4.072044
PRIMER_LEFT_32_PENALTY=1.104440
PRIMER_RIGHT_32_PENALTY=2.967603
PRIMER_LEFT_32_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_32_SEQUENCE=TAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_32=797,20
PRIMER_RIGHT_32=1784,20
PRIMER_LEFT_32_TM=58.896
PRIMER_RIGHT_32_TM=57.032
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=988
PRIMER_PAIR_33_PENALTY=4.085757
PRIMER_LEFT_33_PENALTY=1.722483
PRIMER_RIGHT_33_PENALTY=2.363274
PRIMER_LEFT_33_SEQUENCE=TTGGAACGTGTGAGATGAGAA
PRIMER_RIGHT_33_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_33=789,21
PRIMER_RIGHT_33=1786,20
PRIMER_LEFT_33_TM=59.278
PRIMER_RIGHT_33_TM=57.637
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=998
PRIMER_PAIR_34_PENALTY=4.106030
PRIMER_LEFT_34_PENALTY=1.560956
PRIMER_RIGHT_34_PENALTY=2.545073
PRIMER_LEFT_34_SEQUENCE=ACCTTGGAACGTGTGAGATGA
PRIMER_RIGHT_34_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_34=786,21
PRIMER_RIGHT_34=1785,21
PRIMER_LEFT_34_TM=60.561
PRIMER_RIGHT_34_TM=58.455
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.6000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=1000
PRIMER_PAIR_35_PENALTY=4.114115
PRIMER_LEFT_35_PENALTY=1.146512
PRIMER_RIGHT_35_PENALTY=2.967603
PRIMER_LEFT_35_SEQUENCE=TGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_35_SEQUENCE=TAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_35=797,21
PRIMER_RIGHT_35=1784,20
PRIMER_LEFT_35_TM=59.853
PRIMER_RIGHT_35_TM=57.032
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.8000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=988
PRIMER_PAIR_36_PENALTY=4.114115
PRIMER_LEFT_36_PENALTY=1.146512
PRIMER_RIGHT_36_PENALTY=2.967603
PRIMER_LEFT_36_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_36_SEQUENCE=TAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_36=796,21
PRIMER_RIGHT_36=1784,20
PRIMER_LEFT_36_TM=59.853
PRIMER_RIGHT_36_TM=57.032
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.4000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=989
PRIMER_PAIR_37_PENALTY=4.240445
PRIMER_LEFT_37_PENALTY=1.695371
PRIMER_RIGHT_37_PENALTY=2.545073
PRIMER_LEFT_37_SEQUENCE=CCTTGGAACGTGTGAGATGAG
PRIMER_RIGHT_37_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_37=787,21
PRIMER_RIGHT_37=1785,21
PRIMER_LEFT_37_TM=60.695
PRIMER_RIGHT_37_TM=58.455
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=999
PRIMER_PAIR_38_PENALTY=4.267556
PRIMER_LEFT_38_PENALTY=1.722483
PRIMER_RIGHT_38_PENALTY=2.545073
PRIMER_LEFT_38_SEQUENCE=TTGGAACGTGTGAGATGAGAA
PRIMER_RIGHT_38_SEQUENCE=TTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_38=789,21
PRIMER_RIGHT_38=1785,21
PRIMER_LEFT_38_TM=59.278
PRIMER_RIGHT_38_TM=58.455
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=997
PRIMER_PAIR_39_PENALTY=4.396468
PRIMER_LEFT_39_PENALTY=2.133445
PRIMER_RIGHT_39_PENALTY=2.263023
PRIMER_LEFT_39_SEQUENCE=CTTGGAACGTGTGAGATGAGA
PRIMER_RIGHT_39_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_39=788,21
PRIMER_RIGHT_39=1786,22
PRIMER_LEFT_39_TM=58.867
PRIMER_RIGHT_39_TM=59.737
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=999
PRIMER_PAIR_40_PENALTY=4.401245
PRIMER_LEFT_40_PENALTY=1.433642
PRIMER_RIGHT_40_PENALTY=2.967603
PRIMER_LEFT_40_SEQUENCE=ACCTTGGAACGTGTGAGATG
PRIMER_RIGHT_40_SEQUENCE=TAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_40=786,20
PRIMER_RIGHT_40=1784,20
PRIMER_LEFT_40_TM=58.566
PRIMER_RIGHT_40_TM=57.032
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.6000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=999
PRIMER_PAIR_41_PENALTY=4.496719
PRIMER_LEFT_41_PENALTY=2.133445
PRIMER_RIGHT_41_PENALTY=2.363274
PRIMER_LEFT_41_SEQUENCE=CTTGGAACGTGTGAGATGAGA
PRIMER_RIGHT_41_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_41=788,21
PRIMER_RIGHT_41=1786,20
PRIMER_LEFT_41_TM=58.867
PRIMER_RIGHT_41_TM=57.637
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=999
PRIMER_PAIR_42_PENALTY=4.528560
PRIMER_LEFT_42_PENALTY=1.560956
PRIMER_RIGHT_42_PENALTY=2.967603
PRIMER_LEFT_42_SEQUENCE=ACCTTGGAACGTGTGAGATGA
PRIMER_RIGHT_42_SEQUENCE=TAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_42=786,21
PRIMER_RIGHT_42=1784,20
PRIMER_LEFT_42_TM=60.561
PRIMER_RIGHT_42_TM=57.032
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=999
PRIMER_PAIR_43_PENALTY=4.535820
PRIMER_LEFT_43_PENALTY=1.104440
PRIMER_RIGHT_43_PENALTY=3.431380
PRIMER_LEFT_43_SEQUENCE=TGTGAGATGAGAATGCACGA
PRIMER_RIGHT_43_SEQUENCE=TAGTCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_43=797,20
PRIMER_RIGHT_43=1793,23
PRIMER_LEFT_43_TM=58.896
PRIMER_RIGHT_43_TM=59.569
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=43.478
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=997
PRIMER_PAIR_44_PENALTY=4.549315
PRIMER_LEFT_44_PENALTY=2.286292
PRIMER_RIGHT_44_PENALTY=2.263023
PRIMER_LEFT_44_SEQUENCE=TGGAACGTGTGAGATGAGAA
PRIMER_RIGHT_44_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_44=790,20
PRIMER_RIGHT_44=1786,22
PRIMER_LEFT_44_TM=57.714
PRIMER_RIGHT_44_TM=59.737
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=997
PRIMER_PAIR_45_PENALTY=4.549315
PRIMER_LEFT_45_PENALTY=2.286292
PRIMER_RIGHT_45_PENALTY=2.263023
PRIMER_LEFT_45_SEQUENCE=TTGGAACGTGTGAGATGAGA
PRIMER_RIGHT_45_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_45=789,20
PRIMER_RIGHT_45=1786,22
PRIMER_LEFT_45_TM=57.714
PRIMER_RIGHT_45_TM=59.737
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=998
PRIMER_PAIR_46_PENALTY=4.553612
PRIMER_LEFT_46_PENALTY=2.290589
PRIMER_RIGHT_46_PENALTY=2.263023
PRIMER_LEFT_46_SEQUENCE=CTTGGAACGTGTGAGATGAGAA
PRIMER_RIGHT_46_SEQUENCE=TTTAGAGAGCCGTGGAAAAATC
PRIMER_LEFT_46=788,22
PRIMER_RIGHT_46=1786,22
PRIMER_LEFT_46_TM=60.291
PRIMER_RIGHT_46_TM=59.737
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=999
PRIMER_PAIR_47_PENALTY=4.577892
PRIMER_LEFT_47_PENALTY=1.146512
PRIMER_RIGHT_47_PENALTY=3.431380
PRIMER_LEFT_47_SEQUENCE=GTGTGAGATGAGAATGCACGA
PRIMER_RIGHT_47_SEQUENCE=TAGTCTTTTTAGAGAGCCGTGGA
PRIMER_LEFT_47=796,21
PRIMER_RIGHT_47=1793,23
PRIMER_LEFT_47_TM=59.853
PRIMER_RIGHT_47_TM=59.569
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=998
PRIMER_PAIR_48_PENALTY=4.634228
PRIMER_LEFT_48_PENALTY=2.731754
PRIMER_RIGHT_48_PENALTY=1.902474
PRIMER_LEFT_48_SEQUENCE=GTGTGAGATGAGAATGCACGAC
PRIMER_RIGHT_48_SEQUENCE=TCTTTTTAGAGAGCCGTGGAA
PRIMER_LEFT_48=796,22
PRIMER_RIGHT_48=1790,21
PRIMER_LEFT_48_TM=60.732
PRIMER_RIGHT_48_TM=59.098
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.8000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=995
PRIMER_PAIR_49_PENALTY=4.649567
PRIMER_LEFT_49_PENALTY=2.286292
PRIMER_RIGHT_49_PENALTY=2.363274
PRIMER_LEFT_49_SEQUENCE=TGGAACGTGTGAGATGAGAA
PRIMER_RIGHT_49_SEQUENCE=TTTAGAGAGCCGTGGAAAAA
PRIMER_LEFT_49=790,20
PRIMER_RIGHT_49=1786,20
PRIMER_LEFT_49_TM=57.714
PRIMER_RIGHT_49_TM=57.637
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=997
=
SEQUENCE_ID=MAGIC.426_2_10638913_10638951_rsch-4_oy-0_169912_120098_TRUE
SEQUENCE_TARGET=950,138
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAACTNAGNAACTGTAGTTTGGGCTCTAATTNTCNATAACTTTTCAATGGATGNAAAGAGGAAATNNTCANAAGAGAAAGGTACATTATAAATATTTCTTGNTATGCCTATACAATTTATATTNTTNNNAAATATTGTATACTGCAAAATCACTTNCAGTTTATNAATGTCTAAATCNTNNTTTTGATAATGATTGATTCATAAGAGNNNNNNNNGAGGATAACCATACCTCCCTCTAGTGAAAAAAACTTTAANTAGAATTANAATTTTATGATTTTNTGATATTTCACATTTTACTTATATATAATCANTNTATTTTATATTTTCAGACNTGTATCAAAACGACGATAAAANGAAAAAGNTCGTGGCANTAGAAAATCAAGAAGCTNGGGGAAGTTGTATCGATGGAAATGTGGCTCATTGGTTTTCGANAACTAAGTTCAATGGGTTGGTGAACGTACCAATTGTGTATTTGGCCTTGGAAGAAAATATAGTTAAGAATGATCTTACATTGAGAAGCTCACTTAAACATGTCATCTTTGGCGGTAGCAGNGGAGGCANTAANANTGGTAAAGGGCTAGCCAAGGATTTGTACTTAGATGTAAGTGTANATATCGAATAANAAATGTGTGGTAGTAAAATATTTCTGTTTGTTNTTGTTATGATTCNATCAAACATATATTCTTGATGAGTTAAATTTTCATATTTTTAGGGAAANGAATTGGTNGAAGNTCATGTGAGAAGTGATAGAGAAATCAATANGATGAAAGACAAAGGAAAANCAATATTCGACCTTAACGAAGNTGTTTGGCCNAGAGATGAANNAGTATCAAACACATTTGAGGTTACCATTTGTNACTGACAANTTTTTTAGGTAAACACATAGGGNAAGATTGTTATTAATGAGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17928109_17928167_po-0_tsu-0_94027_10337_TRUE
SEQUENCE_TARGET=950,158
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGAGATTCNACGNTTTANTAAAANTTTAAAGAGAATAATAATAATTTTTTCNCTCTTAGACAAAANNAAATCANTAAANTTTTTTCAATNAANAATAATAAANTNNNNNNNNTATACTATATAAATTAATTTGACAAATGGTGNNNAAAAAAAANAGGCAATANACCTATGTTATGTATAGCCNAACCCATTCTAACTAGGCCCAAATTGAAACAAAGCCCAANAAGAAGGAAAGTGTNATTCATTCTTTCNCGAATAAAACCNTAGAAATTCCCCCTNTTCCCCAATTCNACTGCCAAAACTCTTCNCTTAATTCTCCGGCGTATACAAGTCGCCGGCAAAAATGTCGATGTCGAAGAGCTCNAAGATGCTTCAGTTCATAAACTACAGGATGCGAGTAACAATCCAAGACGGAAGACAGCTAATCGGAAAATTCATGGCGTTCGATCGCCACATGAATCTTGTTCTNGGCGACTGCGAAGAGTTTCGAAAACTTCCTCCGGCGAAAGGTAANAAGAANACGANCGAAGAAANAGAAGAGCGTCGTACNCTNGGTTTGGTTTTACTTAGAGGAGAAGAAGTGATTTCGATGACTGTCGAAGGTCCACCGCCACCNGAAGAATCTCGCGCCAAATCTGGATCTGTNACCGCNGTGGCTGGTCCNGGAATCGGTCGTGCTGCTGGACGNGGAGTACCTACTGGTCCNTTNGTTCAAGCTCAGNCTGGTTTATCTGGTCCTGTTCGTGGAATTGGTGGACCTGCTCNTGGAATGATGCANCCTCAGATCTCTAGACCACCACAGATTATTCGTCCTCCGGGACAAATGCCACCACNNCCTCCTTTTGCTGGTCAAGGAGGTCCTCCTCCACCTTATGGTATGAGACCACCGTATCCTGGTCCACCACCGCCTCNNTATGGTGGACANCAAAGGCCAATGATGATTCCTCNACCTGGTGGTATGATGAGAGGACCTCCTCCACCTCATGGTATGCAAGGACCGCCTCCNNCTCGCCCTGGAATGCCTCCNCCNGGTGGTGCTCCGATGTTTGCTCCNCCTCATCCTGGCATGCCACCTGCTCCGCCAAATCACCATAATCAGCAACATTGATTAGNTAGCTGTTTATCAANTTTTGGNACACTNCTTGATTATCCTTTATTATCTGCTGATGGTTGNTTNTAAGTGTAGCTTGGCTATGATGTGAGATTTGGTTGAAATTGAACTTATTTTGATTTAGCTTGGACTTGTGTAGTGACTGAAGTTTATAAATCATAAGTTAGCTGCAGTCTCTACTAGTCTGCTGATAGTTTANTTGTGAAACCTTTAACTTACACAAAAACTTTCTTGTAACNTGAACTGATAACNTACACAGAAATTCTACTTAATTAGCCTTTATAATTTATCAGCAATTTCTAGTCTGCTTGGCCATGTGTGATTTGTNTGGAATTGAACTTGTCTTGATTAGCTTGGGCATGTGNTGTNAGTAGTAGTCTGCNCATGACTGTCTTAGCTTGCANAGTTTCNAGGTTGTTGATGAGTGCTTTGTTGTCAGTTGCTTTTCTTCTTATGTTTCCTTAAGAGTATGATCACTNNNNNNNNNNNNNNNNNNNNNNNNATTCTAGAATCATCACATNNNNAATCCTGTAACTTACACTCANAAACTCTNTTGCNTGTTGTTCTAANGAGTTTGAAAANNCNNNNNNNNNNNTTTAGAAGCATGAATAACTCATGATGAACTTCTCANTTCACTTTTAAACGTNTTTAANATCTTCCCAACTAGTANAACNTTTATGATCCTTGTTTAAGGTTTGTTCGTGAATTTTGTTTTGGAAAGCTGTAGATGTTGTAGGGTTCATTAGCTATATTGGAAGAACTTGTGNATACATCNTTTTGCCCTTGCTCTCTGTTGGCATATGATATTGGATGANTTTTTTTTNNNNATATCTCTGTTNGAAAGATCTGCTNAGTACAATTTTCTCGATCTGGATTGTAGGAGTCACTNCATCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.379209
PRIMER_LEFT_0_PENALTY=0.163658
PRIMER_RIGHT_0_PENALTY=0.215550
PRIMER_LEFT_0_SEQUENCE=GACGGAAGACAGCTAATCGG
PRIMER_RIGHT_0_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_0=462,20
PRIMER_RIGHT_0=1148,20
PRIMER_LEFT_0_TM=59.836
PRIMER_RIGHT_0_TM=59.784
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.8000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=687
PRIMER_PAIR_1_PENALTY=0.485009
PRIMER_LEFT_1_PENALTY=0.173087
PRIMER_RIGHT_1_PENALTY=0.311923
PRIMER_LEFT_1_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_1_SEQUENCE=CACATGCCCAAGCTAATCAA
PRIMER_LEFT_1=787,20
PRIMER_RIGHT_1=1527,20
PRIMER_LEFT_1_TM=59.827
PRIMER_RIGHT_1_TM=59.688
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=741
PRIMER_PAIR_2_PENALTY=0.559500
PRIMER_LEFT_2_PENALTY=0.343950
PRIMER_RIGHT_2_PENALTY=0.215550
PRIMER_LEFT_2_SEQUENCE=CGGAAGACAGCTAATCGGAA
PRIMER_RIGHT_2_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_2=464,20
PRIMER_RIGHT_2=1148,20
PRIMER_LEFT_2_TM=60.344
PRIMER_RIGHT_2_TM=59.784
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=685
PRIMER_PAIR_3_PENALTY=0.753253
PRIMER_LEFT_3_PENALTY=0.173087
PRIMER_RIGHT_3_PENALTY=0.580167
PRIMER_LEFT_3_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_3_SEQUENCE=TGCCCAAGCTAATCAAGACA
PRIMER_LEFT_3=787,20
PRIMER_RIGHT_3=1523,20
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=59.420
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=737
PRIMER_PAIR_4_PENALTY=0.833411
PRIMER_LEFT_4_PENALTY=0.163658
PRIMER_RIGHT_4_PENALTY=0.669753
PRIMER_LEFT_4_SEQUENCE=GACGGAAGACAGCTAATCGG
PRIMER_RIGHT_4_SEQUENCE=GATTATGGTGATTTGGCGGA
PRIMER_LEFT_4=462,20
PRIMER_RIGHT_4=1149,20
PRIMER_LEFT_4_TM=59.836
PRIMER_RIGHT_4_TM=60.670
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=688
PRIMER_PAIR_5_PENALTY=0.946617
PRIMER_LEFT_5_PENALTY=0.366450
PRIMER_RIGHT_5_PENALTY=0.580167
PRIMER_LEFT_5_SEQUENCE=TCCTTTTGCTGGTCAAGGAG
PRIMER_RIGHT_5_SEQUENCE=TGCCCAAGCTAATCAAGACA
PRIMER_LEFT_5=890,20
PRIMER_RIGHT_5=1523,20
PRIMER_LEFT_5_TM=60.366
PRIMER_RIGHT_5_TM=59.420
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=634
PRIMER_PAIR_6_PENALTY=0.983520
PRIMER_LEFT_6_PENALTY=0.671597
PRIMER_RIGHT_6_PENALTY=0.311923
PRIMER_LEFT_6_SEQUENCE=CTTTTGCTGGTCAAGGAGGT
PRIMER_RIGHT_6_SEQUENCE=CACATGCCCAAGCTAATCAA
PRIMER_LEFT_6=892,20
PRIMER_RIGHT_6=1527,20
PRIMER_LEFT_6_TM=59.328
PRIMER_RIGHT_6_TM=59.688
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=636
PRIMER_PAIR_7_PENALTY=1.013703
PRIMER_LEFT_7_PENALTY=0.343950
PRIMER_RIGHT_7_PENALTY=0.669753
PRIMER_LEFT_7_SEQUENCE=CGGAAGACAGCTAATCGGAA
PRIMER_RIGHT_7_SEQUENCE=GATTATGGTGATTTGGCGGA
PRIMER_LEFT_7=464,20
PRIMER_RIGHT_7=1149,20
PRIMER_LEFT_7_TM=60.344
PRIMER_RIGHT_7_TM=60.670
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=10.2000
PRIMER_RIGHT_7_END_STABILITY=11.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=686
PRIMER_PAIR_8_PENALTY=1.051944
PRIMER_LEFT_8_PENALTY=0.836394
PRIMER_RIGHT_8_PENALTY=0.215550
PRIMER_LEFT_8_SEQUENCE=CCAAATTGAAACAAAGCCCA
PRIMER_RIGHT_8_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_8=255,20
PRIMER_RIGHT_8=1148,20
PRIMER_LEFT_8_TM=60.836
PRIMER_RIGHT_8_TM=59.784
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=11.2000
PRIMER_RIGHT_8_END_STABILITY=9.9000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=894
PRIMER_PAIR_9_PENALTY=1.148704
PRIMER_LEFT_9_PENALTY=0.173087
PRIMER_RIGHT_9_PENALTY=0.975617
PRIMER_LEFT_9_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_9_SEQUENCE=CATGGCCAAGCAGACTAGAA
PRIMER_LEFT_9=787,20
PRIMER_RIGHT_9=1480,20
PRIMER_LEFT_9_TM=59.827
PRIMER_RIGHT_9_TM=59.024
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=694
PRIMER_PAIR_10_PENALTY=1.151737
PRIMER_LEFT_10_PENALTY=0.936187
PRIMER_RIGHT_10_PENALTY=0.215550
PRIMER_LEFT_10_SEQUENCE=AGGCCCAAATTGAAACAAAG
PRIMER_RIGHT_10_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_10=251,20
PRIMER_RIGHT_10=1148,20
PRIMER_LEFT_10_TM=59.064
PRIMER_RIGHT_10_TM=59.784
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=9.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=898
PRIMER_PAIR_11_PENALTY=1.216231
PRIMER_LEFT_11_PENALTY=1.000680
PRIMER_RIGHT_11_PENALTY=0.215550
PRIMER_LEFT_11_SEQUENCE=TCCAAGACGGAAGACAGCTAA
PRIMER_RIGHT_11_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_11=457,21
PRIMER_RIGHT_11=1148,20
PRIMER_LEFT_11_TM=60.001
PRIMER_RIGHT_11_TM=59.784
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.5000
PRIMER_RIGHT_11_END_STABILITY=9.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=692
PRIMER_PAIR_12_PENALTY=1.225979
PRIMER_LEFT_12_PENALTY=0.914056
PRIMER_RIGHT_12_PENALTY=0.311923
PRIMER_LEFT_12_SEQUENCE=TATCTGGTCCTGTTCGTGGA
PRIMER_RIGHT_12_SEQUENCE=CACATGCCCAAGCTAATCAA
PRIMER_LEFT_12=781,20
PRIMER_RIGHT_12=1527,20
PRIMER_LEFT_12_TM=59.086
PRIMER_RIGHT_12_TM=59.688
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=747
PRIMER_PAIR_13_PENALTY=1.251764
PRIMER_LEFT_13_PENALTY=0.671597
PRIMER_RIGHT_13_PENALTY=0.580167
PRIMER_LEFT_13_SEQUENCE=CTTTTGCTGGTCAAGGAGGT
PRIMER_RIGHT_13_SEQUENCE=TGCCCAAGCTAATCAAGACA
PRIMER_LEFT_13=892,20
PRIMER_RIGHT_13=1523,20
PRIMER_LEFT_13_TM=59.328
PRIMER_RIGHT_13_TM=59.420
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=632
PRIMER_PAIR_14_PENALTY=1.283186
PRIMER_LEFT_14_PENALTY=0.173087
PRIMER_RIGHT_14_PENALTY=1.110099
PRIMER_LEFT_14_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_14_SEQUENCE=ACATGGCCAAGCAGACTAGAA
PRIMER_LEFT_14=787,20
PRIMER_RIGHT_14=1481,21
PRIMER_LEFT_14_TM=59.827
PRIMER_RIGHT_14_TM=59.890
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=695
PRIMER_PAIR_15_PENALTY=1.333183
PRIMER_LEFT_15_PENALTY=1.117633
PRIMER_RIGHT_15_PENALTY=0.215550
PRIMER_LEFT_15_SEQUENCE=CGAGTAACAATCCAAGACGGA
PRIMER_RIGHT_15_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_15=447,21
PRIMER_RIGHT_15=1148,20
PRIMER_LEFT_15_TM=60.118
PRIMER_RIGHT_15_TM=59.784
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=702
PRIMER_PAIR_16_PENALTY=1.333461
PRIMER_LEFT_16_PENALTY=1.021538
PRIMER_RIGHT_16_PENALTY=0.311923
PRIMER_LEFT_16_SEQUENCE=TTTATCTGGTCCTGTTCGTGG
PRIMER_RIGHT_16_SEQUENCE=CACATGCCCAAGCTAATCAA
PRIMER_LEFT_16=779,21
PRIMER_RIGHT_16=1527,20
PRIMER_LEFT_16_TM=59.978
PRIMER_RIGHT_16_TM=59.688
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=749
PRIMER_PAIR_17_PENALTY=1.451374
PRIMER_LEFT_17_PENALTY=0.173087
PRIMER_RIGHT_17_PENALTY=1.278288
PRIMER_LEFT_17_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_17_SEQUENCE=ATGCCCAAGCTAATCAAGACA
PRIMER_LEFT_17=787,20
PRIMER_RIGHT_17=1524,21
PRIMER_LEFT_17_TM=59.827
PRIMER_RIGHT_17_TM=59.722
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=738
PRIMER_PAIR_18_PENALTY=1.451374
PRIMER_LEFT_18_PENALTY=0.173087
PRIMER_RIGHT_18_PENALTY=1.278288
PRIMER_LEFT_18_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_18_SEQUENCE=CATGCCCAAGCTAATCAAGAC
PRIMER_LEFT_18=787,20
PRIMER_RIGHT_18=1525,21
PRIMER_LEFT_18_TM=59.827
PRIMER_RIGHT_18_TM=59.722
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=739
PRIMER_PAIR_19_PENALTY=1.494223
PRIMER_LEFT_19_PENALTY=0.914056
PRIMER_RIGHT_19_PENALTY=0.580167
PRIMER_LEFT_19_SEQUENCE=TATCTGGTCCTGTTCGTGGA
PRIMER_RIGHT_19_SEQUENCE=TGCCCAAGCTAATCAAGACA
PRIMER_LEFT_19=781,20
PRIMER_RIGHT_19=1523,20
PRIMER_LEFT_19_TM=59.086
PRIMER_RIGHT_19_TM=59.420
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=743
PRIMER_PAIR_20_PENALTY=1.506147
PRIMER_LEFT_20_PENALTY=0.836394
PRIMER_RIGHT_20_PENALTY=0.669753
PRIMER_LEFT_20_SEQUENCE=CCAAATTGAAACAAAGCCCA
PRIMER_RIGHT_20_SEQUENCE=GATTATGGTGATTTGGCGGA
PRIMER_LEFT_20=255,20
PRIMER_RIGHT_20=1149,20
PRIMER_LEFT_20_TM=60.836
PRIMER_RIGHT_20_TM=60.670
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=11.2000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=895
PRIMER_PAIR_21_PENALTY=1.565688
PRIMER_LEFT_21_PENALTY=0.173087
PRIMER_RIGHT_21_PENALTY=1.392601
PRIMER_LEFT_21_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_21_SEQUENCE=CATGGCCAAGCAGACTAGAAA
PRIMER_LEFT_21=787,20
PRIMER_RIGHT_21=1480,21
PRIMER_LEFT_21_TM=59.827
PRIMER_RIGHT_21_TM=60.393
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=694
PRIMER_PAIR_22_PENALTY=1.601705
PRIMER_LEFT_22_PENALTY=1.021538
PRIMER_RIGHT_22_PENALTY=0.580167
PRIMER_LEFT_22_SEQUENCE=TTTATCTGGTCCTGTTCGTGG
PRIMER_RIGHT_22_SEQUENCE=TGCCCAAGCTAATCAAGACA
PRIMER_LEFT_22=779,21
PRIMER_RIGHT_22=1523,20
PRIMER_LEFT_22_TM=59.978
PRIMER_RIGHT_22_TM=59.420
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.9000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=745
PRIMER_PAIR_23_PENALTY=1.605940
PRIMER_LEFT_23_PENALTY=0.936187
PRIMER_RIGHT_23_PENALTY=0.669753
PRIMER_LEFT_23_SEQUENCE=AGGCCCAAATTGAAACAAAG
PRIMER_RIGHT_23_SEQUENCE=GATTATGGTGATTTGGCGGA
PRIMER_LEFT_23=251,20
PRIMER_RIGHT_23=1149,20
PRIMER_LEFT_23_TM=59.064
PRIMER_RIGHT_23_TM=60.670
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=11.4000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=899
PRIMER_PAIR_24_PENALTY=1.632517
PRIMER_LEFT_24_PENALTY=1.416967
PRIMER_RIGHT_24_PENALTY=0.215550
PRIMER_LEFT_24_SEQUENCE=GGATGCGAGTAACAATCCAAG
PRIMER_RIGHT_24_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_24=442,21
PRIMER_RIGHT_24=1148,20
PRIMER_LEFT_24_TM=59.583
PRIMER_RIGHT_24_TM=59.784
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=9.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=707
PRIMER_PAIR_25_PENALTY=1.632517
PRIMER_LEFT_25_PENALTY=1.416967
PRIMER_RIGHT_25_PENALTY=0.215550
PRIMER_LEFT_25_SEQUENCE=AGGATGCGAGTAACAATCCAA
PRIMER_RIGHT_25_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_25=441,21
PRIMER_RIGHT_25=1148,20
PRIMER_LEFT_25_TM=59.583
PRIMER_RIGHT_25_TM=59.784
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=708
PRIMER_PAIR_26_PENALTY=1.632534
PRIMER_LEFT_26_PENALTY=1.416984
PRIMER_RIGHT_26_PENALTY=0.215550
PRIMER_LEFT_26_SEQUENCE=AACAATCCAAGACGGAAGACA
PRIMER_RIGHT_26_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_26=452,21
PRIMER_RIGHT_26=1148,20
PRIMER_LEFT_26_TM=59.583
PRIMER_RIGHT_26_TM=59.784
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=697
PRIMER_PAIR_27_PENALTY=1.641849
PRIMER_LEFT_27_PENALTY=1.426299
PRIMER_RIGHT_27_PENALTY=0.215550
PRIMER_LEFT_27_SEQUENCE=GGATGCGAGTAACAATCCAA
PRIMER_RIGHT_27_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_27=442,20
PRIMER_RIGHT_27=1148,20
PRIMER_LEFT_27_TM=58.574
PRIMER_RIGHT_27_TM=59.784
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=9.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=707
PRIMER_PAIR_28_PENALTY=1.644738
PRIMER_LEFT_28_PENALTY=0.366450
PRIMER_RIGHT_28_PENALTY=1.278288
PRIMER_LEFT_28_SEQUENCE=TCCTTTTGCTGGTCAAGGAG
PRIMER_RIGHT_28_SEQUENCE=ATGCCCAAGCTAATCAAGACA
PRIMER_LEFT_28=890,20
PRIMER_RIGHT_28=1524,21
PRIMER_LEFT_28_TM=60.366
PRIMER_RIGHT_28_TM=59.722
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=635
PRIMER_PAIR_29_PENALTY=1.644738
PRIMER_LEFT_29_PENALTY=0.366450
PRIMER_RIGHT_29_PENALTY=1.278288
PRIMER_LEFT_29_SEQUENCE=TCCTTTTGCTGGTCAAGGAG
PRIMER_RIGHT_29_SEQUENCE=CATGCCCAAGCTAATCAAGAC
PRIMER_LEFT_29=890,20
PRIMER_RIGHT_29=1525,21
PRIMER_LEFT_29_TM=60.366
PRIMER_RIGHT_29_TM=59.722
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=636
PRIMER_PAIR_30_PENALTY=1.647214
PRIMER_LEFT_30_PENALTY=0.671597
PRIMER_RIGHT_30_PENALTY=0.975617
PRIMER_LEFT_30_SEQUENCE=CTTTTGCTGGTCAAGGAGGT
PRIMER_RIGHT_30_SEQUENCE=CATGGCCAAGCAGACTAGAA
PRIMER_LEFT_30=892,20
PRIMER_RIGHT_30=1480,20
PRIMER_LEFT_30_TM=59.328
PRIMER_RIGHT_30_TM=59.024
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=6.0000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=589
PRIMER_PAIR_31_PENALTY=1.670433
PRIMER_LEFT_31_PENALTY=1.000680
PRIMER_RIGHT_31_PENALTY=0.669753
PRIMER_LEFT_31_SEQUENCE=TCCAAGACGGAAGACAGCTAA
PRIMER_RIGHT_31_SEQUENCE=GATTATGGTGATTTGGCGGA
PRIMER_LEFT_31=457,21
PRIMER_RIGHT_31=1149,20
PRIMER_LEFT_31_TM=60.001
PRIMER_RIGHT_31_TM=60.670
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=11.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=693
PRIMER_PAIR_32_PENALTY=1.743327
PRIMER_LEFT_32_PENALTY=1.431404
PRIMER_RIGHT_32_PENALTY=0.311923
PRIMER_LEFT_32_SEQUENCE=TTATCTGGTCCTGTTCGTGG
PRIMER_RIGHT_32_SEQUENCE=CACATGCCCAAGCTAATCAA
PRIMER_LEFT_32=780,20
PRIMER_RIGHT_32=1527,20
PRIMER_LEFT_32_TM=58.569
PRIMER_RIGHT_32_TM=59.688
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.9000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=748
PRIMER_PAIR_33_PENALTY=1.759052
PRIMER_LEFT_33_PENALTY=0.366450
PRIMER_RIGHT_33_PENALTY=1.392601
PRIMER_LEFT_33_SEQUENCE=TCCTTTTGCTGGTCAAGGAG
PRIMER_RIGHT_33_SEQUENCE=CATGGCCAAGCAGACTAGAAA
PRIMER_LEFT_33=890,20
PRIMER_RIGHT_33=1480,21
PRIMER_LEFT_33_TM=60.366
PRIMER_RIGHT_33_TM=60.393
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=591
PRIMER_PAIR_34_PENALTY=1.762599
PRIMER_LEFT_34_PENALTY=1.547049
PRIMER_RIGHT_34_PENALTY=0.215550
PRIMER_LEFT_34_SEQUENCE=CCCAAATTGAAACAAAGCCC
PRIMER_RIGHT_34_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_34=254,20
PRIMER_RIGHT_34=1148,20
PRIMER_LEFT_34_TM=61.547
PRIMER_RIGHT_34_TM=59.784
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.9000
PRIMER_RIGHT_34_END_STABILITY=9.9000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=895
PRIMER_PAIR_35_PENALTY=1.769860
PRIMER_LEFT_35_PENALTY=1.554309
PRIMER_RIGHT_35_PENALTY=0.215550
PRIMER_LEFT_35_SEQUENCE=AATCGGAAAATTCATGGCGT
PRIMER_RIGHT_35_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_35=476,20
PRIMER_RIGHT_35=1148,20
PRIMER_LEFT_35_TM=61.554
PRIMER_RIGHT_35_TM=59.784
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=11.1000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=673
PRIMER_PAIR_36_PENALTY=1.781696
PRIMER_LEFT_36_PENALTY=0.671597
PRIMER_RIGHT_36_PENALTY=1.110099
PRIMER_LEFT_36_SEQUENCE=CTTTTGCTGGTCAAGGAGGT
PRIMER_RIGHT_36_SEQUENCE=ACATGGCCAAGCAGACTAGAA
PRIMER_LEFT_36=892,20
PRIMER_RIGHT_36=1481,21
PRIMER_LEFT_36_TM=59.328
PRIMER_RIGHT_36_TM=59.890
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=6.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=590
PRIMER_PAIR_37_PENALTY=1.787386
PRIMER_LEFT_37_PENALTY=1.117633
PRIMER_RIGHT_37_PENALTY=0.669753
PRIMER_LEFT_37_SEQUENCE=CGAGTAACAATCCAAGACGGA
PRIMER_RIGHT_37_SEQUENCE=GATTATGGTGATTTGGCGGA
PRIMER_LEFT_37=447,21
PRIMER_RIGHT_37=1149,20
PRIMER_LEFT_37_TM=60.118
PRIMER_RIGHT_37_TM=60.670
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=11.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=703
PRIMER_PAIR_38_PENALTY=1.813290
PRIMER_LEFT_38_PENALTY=1.501367
PRIMER_RIGHT_38_PENALTY=0.311923
PRIMER_LEFT_38_SEQUENCE=TTATCTGGTCCTGTTCGTGGA
PRIMER_RIGHT_38_SEQUENCE=CACATGCCCAAGCTAATCAA
PRIMER_LEFT_38=780,21
PRIMER_RIGHT_38=1527,20
PRIMER_LEFT_38_TM=60.501
PRIMER_RIGHT_38_TM=59.688
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=748
PRIMER_PAIR_39_PENALTY=1.816048
PRIMER_LEFT_39_PENALTY=0.173087
PRIMER_RIGHT_39_PENALTY=1.642961
PRIMER_LEFT_39_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_39_SEQUENCE=CACATGCCCAAGCTAATCAAG
PRIMER_LEFT_39=787,20
PRIMER_RIGHT_39=1527,21
PRIMER_LEFT_39_TM=59.827
PRIMER_RIGHT_39_TM=60.643
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=741
PRIMER_PAIR_40_PENALTY=1.889673
PRIMER_LEFT_40_PENALTY=0.914056
PRIMER_RIGHT_40_PENALTY=0.975617
PRIMER_LEFT_40_SEQUENCE=TATCTGGTCCTGTTCGTGGA
PRIMER_RIGHT_40_SEQUENCE=CATGGCCAAGCAGACTAGAA
PRIMER_LEFT_40=781,20
PRIMER_RIGHT_40=1480,20
PRIMER_LEFT_40_TM=59.086
PRIMER_RIGHT_40_TM=59.024
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.0000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=700
PRIMER_PAIR_41_PENALTY=1.901821
PRIMER_LEFT_41_PENALTY=1.686271
PRIMER_RIGHT_41_PENALTY=0.215550
PRIMER_LEFT_41_SEQUENCE=GCCCAAATTGAAACAAAGCC
PRIMER_RIGHT_41_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_41=253,20
PRIMER_RIGHT_41=1148,20
PRIMER_LEFT_41_TM=61.686
PRIMER_RIGHT_41_TM=59.784
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=9.9000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=896
PRIMER_PAIR_42_PENALTY=1.929165
PRIMER_LEFT_42_PENALTY=1.713615
PRIMER_RIGHT_42_PENALTY=0.215550
PRIMER_LEFT_42_SEQUENCE=CAATCCAAGACGGAAGACAG
PRIMER_RIGHT_42_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_42=454,20
PRIMER_RIGHT_42=1148,20
PRIMER_LEFT_42_TM=58.286
PRIMER_RIGHT_42_TM=59.784
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=9.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=695
PRIMER_PAIR_43_PENALTY=1.930226
PRIMER_LEFT_43_PENALTY=0.173087
PRIMER_RIGHT_43_PENALTY=1.757139
PRIMER_LEFT_43_SEQUENCE=GTCCTGTTCGTGGAATTGGT
PRIMER_RIGHT_43_SEQUENCE=TGCCCAAGCTAATCAAGACAA
PRIMER_LEFT_43=787,20
PRIMER_RIGHT_43=1523,21
PRIMER_LEFT_43_TM=59.827
PRIMER_RIGHT_43_TM=60.757
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=737
PRIMER_PAIR_44_PENALTY=1.949885
PRIMER_LEFT_44_PENALTY=0.671597
PRIMER_RIGHT_44_PENALTY=1.278288
PRIMER_LEFT_44_SEQUENCE=CTTTTGCTGGTCAAGGAGGT
PRIMER_RIGHT_44_SEQUENCE=ATGCCCAAGCTAATCAAGACA
PRIMER_LEFT_44=892,20
PRIMER_RIGHT_44=1524,21
PRIMER_LEFT_44_TM=59.328
PRIMER_RIGHT_44_TM=59.722
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=633
PRIMER_PAIR_45_PENALTY=1.949885
PRIMER_LEFT_45_PENALTY=0.671597
PRIMER_RIGHT_45_PENALTY=1.278288
PRIMER_LEFT_45_SEQUENCE=CTTTTGCTGGTCAAGGAGGT
PRIMER_RIGHT_45_SEQUENCE=CATGCCCAAGCTAATCAAGAC
PRIMER_LEFT_45=892,20
PRIMER_RIGHT_45=1525,21
PRIMER_LEFT_45_TM=59.328
PRIMER_RIGHT_45_TM=59.722
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=634
PRIMER_PAIR_46_PENALTY=1.957717
PRIMER_LEFT_46_PENALTY=0.343950
PRIMER_RIGHT_46_PENALTY=1.613767
PRIMER_LEFT_46_SEQUENCE=CGGAAGACAGCTAATCGGAA
PRIMER_RIGHT_46_SEQUENCE=TGATTATGGTGATTTGGCGG
PRIMER_LEFT_46=464,20
PRIMER_RIGHT_46=1150,20
PRIMER_LEFT_46_TM=60.344
PRIMER_RIGHT_46_TM=61.614
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=10.2000
PRIMER_RIGHT_46_END_STABILITY=12.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=687
PRIMER_PAIR_47_PENALTY=1.980504
PRIMER_LEFT_47_PENALTY=1.310751
PRIMER_RIGHT_47_PENALTY=0.669753
PRIMER_LEFT_47_SEQUENCE=CCCATTCTAACTAGGCCCAAA
PRIMER_RIGHT_47_SEQUENCE=GATTATGGTGATTTGGCGGA
PRIMER_LEFT_47=239,21
PRIMER_RIGHT_47=1149,20
PRIMER_LEFT_47_TM=60.311
PRIMER_RIGHT_47_TM=60.670
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.8000
PRIMER_RIGHT_47_END_STABILITY=11.4000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=911
PRIMER_PAIR_48_PENALTY=1.983258
PRIMER_LEFT_48_PENALTY=1.767708
PRIMER_RIGHT_48_PENALTY=0.215550
PRIMER_LEFT_48_SEQUENCE=AGACGGAAGACAGCTAATCGG
PRIMER_RIGHT_48_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_48=461,21
PRIMER_RIGHT_48=1148,20
PRIMER_LEFT_48_TM=60.768
PRIMER_RIGHT_48_TM=59.784
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.8000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=688
PRIMER_PAIR_49_PENALTY=1.987284
PRIMER_LEFT_49_PENALTY=1.771733
PRIMER_RIGHT_49_PENALTY=0.215550
PRIMER_LEFT_49_SEQUENCE=CGAGTAACAATCCAAGACGG
PRIMER_RIGHT_49_SEQUENCE=ATTATGGTGATTTGGCGGAG
PRIMER_LEFT_49=447,20
PRIMER_RIGHT_49=1148,20
PRIMER_LEFT_49_TM=58.228
PRIMER_RIGHT_49_TM=59.784
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=9.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=702
=
SEQUENCE_ID=MAGIC.149_1_29148165_29148364_kn-0_oy-0_351517_132276_TRUE
SEQUENCE_TARGET=950,299
SEQUENCE_TEMPLATE=TAAGGGAAAATCTACCAAAACCCCAAATCCAAGAGAAGGAAATAAAAGCTACGAGGGTAATGGAACAGTNATAAGTAAGTCTCAAACTACACTCCAAACATTGGTCTATTTCAGAGAACACTTAAGTAGATAAATNNNNAANAAACGAACAAAGGAAGAGAATATTGTGAAAGAGACCAAATGTTTGGTGAAGTAAATACCTTTTGGTTGGGGACTGTATGAAGAACTTTGAGATCCCAAAATGCCCCAATGGAAACAAAAAAAAATCAGCGATTAAACAAGCAAAAAGGAAGAAGCTTTCTTCTTCTTCTNNNNNNNNNNNNNNNACTATTACGATTTATTAACAGCGACAAGGCAGAGATATAGCGNNNNAGAGAGAGAGANAGAGAGNNGAGAGAATTCTCTTTATCTTGTTTTTTGCTATTTTTNAAATATTTCTCTAATTTTTCTTCTTTAATTCAAAAGATAGAAAGAGAGATAGAGCATGTGATCTTCTGTATGTGGAGCTGTGAACTNAAAAAACATTTAAATNTTAACTTTTTCNGCTTTTTTTAAGNAATTATTANTTTTNTGTATATAAATGTAAGGAATTAAAAGTTAAAACGTAATTTCGTACTAATTAATTTGAATTATTGACATTTAAAGTGCTTATAACAATTCCCGCCCNAGAATACATTATTGACAATAATTTAGGATAATTAAATTACGANTTTAGAAAGCAGCTTTTGAATTTAAGGAAGTTGTAACGGTTTNNNTAATAATAATGTGATNAAAGAAGAGCTCACGCGTCTCGCTGACTCTGGTCATTGGGTTCACTGACAAAATCAGTGAGATTCACAATTTATCCGACGGTTTAGATTGCTCCCATCTCTCACTCTGCTTTCTACNNNNNNTTTNTNTTTNNTNTGNTAAGTACAATATAATTAATTTATACACTAATCTCNAATTTATCTTTTCTTAATTGTTGGTAGTTGGTAAGATTAACTTTATTTTCACTCCANATTACAATTNATAGTCNCTTTCCTTTTGTCGTTTATCTCGAGTCCCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGACNTGGTTTGATAAAGGCCCATTAAGTTGNATAGANANNGTCTCAAAGCCTNNAAAGATTNNNNNNNNNNNNAGACCCATNACTNTNTTNTTTCNGTCTCGTNNNAGTNCCCNNANNANTTNCACGAAAAGACAGNNACATCANNGAGANAANAGATNACTATTCTTAGAATTAAGTTTTTGTGCNNCTGTTNNGTCTTTCGAATGCATCGAGACGTAANAAAAAANAAAGTAAAGGAAGAAGAAAANTTTAGGCATATAACTTGGTGAGATCTTCAGAGAATTCCCTCCTAATTACNTCTCTTTTNAGCTTTAGAGCTGCTGTGACTAATCCTGACTCTGGCGTCCATGGAGATGCCAANAATTTGATCTTTGCTGGTATCTCAAACTTCTCCAATCGTGATTGTTTANCCGCCTGCATAACANAGATNNTATAAGNAANTGACTNGGGGACTCAANNNNNNNNNNNNNNAGTAGCAATGAACATACCTTNACAAGNGANGCATACACTTCTTTCACGGCTTGCTCTTTCGTGCACAGTTCTTCGAAGTTGGCAAAGTCTATTCCTTGCTTTGAAGCCCAACCTTCAACTGTATGTTGGGACGCGACCACAAGAGCCACACAGTAACTGTAGAACGAATCAGCATGAACCATTATGTTTTCAACATAGGGACTTATACTTAGAGCAGCTTCAACCTGCNNAAAAAAAGAATGTTTTAANAAGCTTCTCCGGAGAATTGAGTGATGAAGANAAAAAANGGNATTCGGATTGAGAAAACTATACTTTGCCCAAGGAGACATATTCTCCNTGCTGAAGTTTAACGATATCCTTTTTTCGGTCTANTATCTCGAGGCAGCCATCAGGGTGAAATCGTCCTATGTCTCCTGTGTAGAACCANCTCATTCCCTTTTCATCAACCTTTGAACATTCAAGTGTATAAAACGGTTAGAGGAAGAACCAAACCAACCAATTCTTTTAACATTGCCNAAACCAATAATGTTGATTTGCCACCTTGTACACTTCTTTAGTTTTCTCCTCATTNTTGAAATACCCAAGCGTGATATTTGAGCCACCAATTACAATTTCACCACGGGGCATNGGCTTATCACTNG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.203945
PRIMER_LEFT_0_PENALTY=0.071684
PRIMER_RIGHT_0_PENALTY=0.132262
PRIMER_LEFT_0_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_0_SEQUENCE=AAGAGCAAGCCGTGAAAGAA
PRIMER_LEFT_0=645,20
PRIMER_RIGHT_0=1615,20
PRIMER_LEFT_0_TM=59.928
PRIMER_RIGHT_0_TM=60.132
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=12.9000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=971
PRIMER_PAIR_1_PENALTY=0.203945
PRIMER_LEFT_1_PENALTY=0.071684
PRIMER_RIGHT_1_PENALTY=0.132262
PRIMER_LEFT_1_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_1_SEQUENCE=AAAGAGCAAGCCGTGAAAGA
PRIMER_LEFT_1=645,20
PRIMER_RIGHT_1=1616,20
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=972
PRIMER_PAIR_2_PENALTY=0.203945
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_2_SEQUENCE=GAAAGAGCAAGCCGTGAAAG
PRIMER_LEFT_2=645,20
PRIMER_RIGHT_2=1617,20
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=973
PRIMER_PAIR_3_PENALTY=0.205876
PRIMER_LEFT_3_PENALTY=0.073614
PRIMER_RIGHT_3_PENALTY=0.132262
PRIMER_LEFT_3_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_3_SEQUENCE=AAGAGCAAGCCGTGAAAGAA
PRIMER_LEFT_3=847,20
PRIMER_RIGHT_3=1615,20
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=769
PRIMER_PAIR_4_PENALTY=0.205876
PRIMER_LEFT_4_PENALTY=0.073614
PRIMER_RIGHT_4_PENALTY=0.132262
PRIMER_LEFT_4_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_4_SEQUENCE=AAAGAGCAAGCCGTGAAAGA
PRIMER_LEFT_4=847,20
PRIMER_RIGHT_4=1616,20
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=770
PRIMER_PAIR_5_PENALTY=0.205876
PRIMER_LEFT_5_PENALTY=0.073614
PRIMER_RIGHT_5_PENALTY=0.132262
PRIMER_LEFT_5_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_5_SEQUENCE=GAAAGAGCAAGCCGTGAAAG
PRIMER_LEFT_5=847,20
PRIMER_RIGHT_5=1617,20
PRIMER_LEFT_5_TM=60.074
PRIMER_RIGHT_5_TM=60.132
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=771
PRIMER_PAIR_6_PENALTY=0.252071
PRIMER_LEFT_6_PENALTY=0.119809
PRIMER_RIGHT_6_PENALTY=0.132262
PRIMER_LEFT_6_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_6_SEQUENCE=AAGAGCAAGCCGTGAAAGAA
PRIMER_LEFT_6=792,20
PRIMER_RIGHT_6=1615,20
PRIMER_LEFT_6_TM=60.120
PRIMER_RIGHT_6_TM=60.132
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=824
PRIMER_PAIR_7_PENALTY=0.252071
PRIMER_LEFT_7_PENALTY=0.119809
PRIMER_RIGHT_7_PENALTY=0.132262
PRIMER_LEFT_7_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_7_SEQUENCE=AAAGAGCAAGCCGTGAAAGA
PRIMER_LEFT_7=792,20
PRIMER_RIGHT_7=1616,20
PRIMER_LEFT_7_TM=60.120
PRIMER_RIGHT_7_TM=60.132
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=825
PRIMER_PAIR_8_PENALTY=0.252071
PRIMER_LEFT_8_PENALTY=0.119809
PRIMER_RIGHT_8_PENALTY=0.132262
PRIMER_LEFT_8_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_8_SEQUENCE=GAAAGAGCAAGCCGTGAAAG
PRIMER_LEFT_8=792,20
PRIMER_RIGHT_8=1617,20
PRIMER_LEFT_8_TM=60.120
PRIMER_RIGHT_8_TM=60.132
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=826
PRIMER_PAIR_9_PENALTY=0.314426
PRIMER_LEFT_9_PENALTY=0.071684
PRIMER_RIGHT_9_PENALTY=0.242742
PRIMER_LEFT_9_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_9_SEQUENCE=AGAGCAAGCCGTGAAAGAAG
PRIMER_LEFT_9=645,20
PRIMER_RIGHT_9=1614,20
PRIMER_LEFT_9_TM=59.928
PRIMER_RIGHT_9_TM=59.757
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=12.9000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=970
PRIMER_PAIR_10_PENALTY=0.316356
PRIMER_LEFT_10_PENALTY=0.073614
PRIMER_RIGHT_10_PENALTY=0.242742
PRIMER_LEFT_10_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_10_SEQUENCE=AGAGCAAGCCGTGAAAGAAG
PRIMER_LEFT_10=847,20
PRIMER_RIGHT_10=1614,20
PRIMER_LEFT_10_TM=60.074
PRIMER_RIGHT_10_TM=59.757
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=768
PRIMER_PAIR_11_PENALTY=0.345767
PRIMER_LEFT_11_PENALTY=0.213506
PRIMER_RIGHT_11_PENALTY=0.132262
PRIMER_LEFT_11_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_11_SEQUENCE=AAGAGCAAGCCGTGAAAGAA
PRIMER_LEFT_11=844,20
PRIMER_RIGHT_11=1615,20
PRIMER_LEFT_11_TM=60.214
PRIMER_RIGHT_11_TM=60.132
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=772
PRIMER_PAIR_12_PENALTY=0.345767
PRIMER_LEFT_12_PENALTY=0.213506
PRIMER_RIGHT_12_PENALTY=0.132262
PRIMER_LEFT_12_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_12_SEQUENCE=AAAGAGCAAGCCGTGAAAGA
PRIMER_LEFT_12=844,20
PRIMER_RIGHT_12=1616,20
PRIMER_LEFT_12_TM=60.214
PRIMER_RIGHT_12_TM=60.132
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=773
PRIMER_PAIR_13_PENALTY=0.345767
PRIMER_LEFT_13_PENALTY=0.213506
PRIMER_RIGHT_13_PENALTY=0.132262
PRIMER_LEFT_13_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_13_SEQUENCE=GAAAGAGCAAGCCGTGAAAG
PRIMER_LEFT_13=844,20
PRIMER_RIGHT_13=1617,20
PRIMER_LEFT_13_TM=60.214
PRIMER_RIGHT_13_TM=60.132
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=774
PRIMER_PAIR_14_PENALTY=0.362551
PRIMER_LEFT_14_PENALTY=0.119809
PRIMER_RIGHT_14_PENALTY=0.242742
PRIMER_LEFT_14_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_14_SEQUENCE=AGAGCAAGCCGTGAAAGAAG
PRIMER_LEFT_14=792,20
PRIMER_RIGHT_14=1614,20
PRIMER_LEFT_14_TM=60.120
PRIMER_RIGHT_14_TM=59.757
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=823
PRIMER_PAIR_15_PENALTY=0.419378
PRIMER_LEFT_15_PENALTY=0.073614
PRIMER_RIGHT_15_PENALTY=0.345764
PRIMER_LEFT_15_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_15_SEQUENCE=TGGTTCATGCTGATTCGTTC
PRIMER_LEFT_15=847,20
PRIMER_RIGHT_15=1738,20
PRIMER_LEFT_15_TM=60.074
PRIMER_RIGHT_15_TM=59.654
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=892
PRIMER_PAIR_16_PENALTY=0.432052
PRIMER_LEFT_16_PENALTY=0.071684
PRIMER_RIGHT_16_PENALTY=0.360369
PRIMER_LEFT_16_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_16_SEQUENCE=AACTGTGCACGAAAGAGCAA
PRIMER_LEFT_16=645,20
PRIMER_RIGHT_16=1627,20
PRIMER_LEFT_16_TM=59.928
PRIMER_RIGHT_16_TM=59.640
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=12.9000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=983
PRIMER_PAIR_17_PENALTY=0.433983
PRIMER_LEFT_17_PENALTY=0.073614
PRIMER_RIGHT_17_PENALTY=0.360369
PRIMER_LEFT_17_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_17_SEQUENCE=AACTGTGCACGAAAGAGCAA
PRIMER_LEFT_17=847,20
PRIMER_RIGHT_17=1627,20
PRIMER_LEFT_17_TM=60.074
PRIMER_RIGHT_17_TM=59.640
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=781
PRIMER_PAIR_18_PENALTY=0.437921
PRIMER_LEFT_18_PENALTY=0.071684
PRIMER_RIGHT_18_PENALTY=0.366237
PRIMER_LEFT_18_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_18_SEQUENCE=CGAAGAACTGTGCACGAAAG
PRIMER_LEFT_18=645,20
PRIMER_RIGHT_18=1632,20
PRIMER_LEFT_18_TM=59.928
PRIMER_RIGHT_18_TM=59.634
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=12.9000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=988
PRIMER_PAIR_19_PENALTY=0.439851
PRIMER_LEFT_19_PENALTY=0.073614
PRIMER_RIGHT_19_PENALTY=0.366237
PRIMER_LEFT_19_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_19_SEQUENCE=CGAAGAACTGTGCACGAAAG
PRIMER_LEFT_19=847,20
PRIMER_RIGHT_19=1632,20
PRIMER_LEFT_19_TM=60.074
PRIMER_RIGHT_19_TM=59.634
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=786
PRIMER_PAIR_20_PENALTY=0.450642
PRIMER_LEFT_20_PENALTY=0.071684
PRIMER_RIGHT_20_PENALTY=0.378958
PRIMER_LEFT_20_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_20_SEQUENCE=GAGCAAGCCGTGAAAGAAGT
PRIMER_LEFT_20=645,20
PRIMER_RIGHT_20=1613,20
PRIMER_LEFT_20_TM=59.928
PRIMER_RIGHT_20_TM=59.621
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=12.9000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=969
PRIMER_PAIR_21_PENALTY=0.452572
PRIMER_LEFT_21_PENALTY=0.073614
PRIMER_RIGHT_21_PENALTY=0.378958
PRIMER_LEFT_21_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_21_SEQUENCE=GAGCAAGCCGTGAAAGAAGT
PRIMER_LEFT_21=847,20
PRIMER_RIGHT_21=1613,20
PRIMER_LEFT_21_TM=60.074
PRIMER_RIGHT_21_TM=59.621
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=767
PRIMER_PAIR_22_PENALTY=0.456248
PRIMER_LEFT_22_PENALTY=0.213506
PRIMER_RIGHT_22_PENALTY=0.242742
PRIMER_LEFT_22_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_22_SEQUENCE=AGAGCAAGCCGTGAAAGAAG
PRIMER_LEFT_22=844,20
PRIMER_RIGHT_22=1614,20
PRIMER_LEFT_22_TM=60.214
PRIMER_RIGHT_22_TM=59.757
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=771
PRIMER_PAIR_23_PENALTY=0.465573
PRIMER_LEFT_23_PENALTY=0.119809
PRIMER_RIGHT_23_PENALTY=0.345764
PRIMER_LEFT_23_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_23_SEQUENCE=TGGTTCATGCTGATTCGTTC
PRIMER_LEFT_23=792,20
PRIMER_RIGHT_23=1738,20
PRIMER_LEFT_23_TM=60.120
PRIMER_RIGHT_23_TM=59.654
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=947
PRIMER_PAIR_24_PENALTY=0.480178
PRIMER_LEFT_24_PENALTY=0.119809
PRIMER_RIGHT_24_PENALTY=0.360369
PRIMER_LEFT_24_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_24_SEQUENCE=AACTGTGCACGAAAGAGCAA
PRIMER_LEFT_24=792,20
PRIMER_RIGHT_24=1627,20
PRIMER_LEFT_24_TM=60.120
PRIMER_RIGHT_24_TM=59.640
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=836
PRIMER_PAIR_25_PENALTY=0.486046
PRIMER_LEFT_25_PENALTY=0.119809
PRIMER_RIGHT_25_PENALTY=0.366237
PRIMER_LEFT_25_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_25_SEQUENCE=CGAAGAACTGTGCACGAAAG
PRIMER_LEFT_25=792,20
PRIMER_RIGHT_25=1632,20
PRIMER_LEFT_25_TM=60.120
PRIMER_RIGHT_25_TM=59.634
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=841
PRIMER_PAIR_26_PENALTY=0.498767
PRIMER_LEFT_26_PENALTY=0.119809
PRIMER_RIGHT_26_PENALTY=0.378958
PRIMER_LEFT_26_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_26_SEQUENCE=GAGCAAGCCGTGAAAGAAGT
PRIMER_LEFT_26=792,20
PRIMER_RIGHT_26=1613,20
PRIMER_LEFT_26_TM=60.120
PRIMER_RIGHT_26_TM=59.621
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=822
PRIMER_PAIR_27_PENALTY=0.512000
PRIMER_LEFT_27_PENALTY=0.071684
PRIMER_RIGHT_27_PENALTY=0.440316
PRIMER_LEFT_27_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_27_SEQUENCE=GCAAGCCGTGAAAGAAGTGT
PRIMER_LEFT_27=645,20
PRIMER_RIGHT_27=1611,20
PRIMER_LEFT_27_TM=59.928
PRIMER_RIGHT_27_TM=60.440
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=12.9000
PRIMER_RIGHT_27_END_STABILITY=6.1000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=967
PRIMER_PAIR_28_PENALTY=0.513930
PRIMER_LEFT_28_PENALTY=0.073614
PRIMER_RIGHT_28_PENALTY=0.440316
PRIMER_LEFT_28_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_28_SEQUENCE=GCAAGCCGTGAAAGAAGTGT
PRIMER_LEFT_28=847,20
PRIMER_RIGHT_28=1611,20
PRIMER_LEFT_28_TM=60.074
PRIMER_RIGHT_28_TM=60.440
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=765
PRIMER_PAIR_29_PENALTY=0.559270
PRIMER_LEFT_29_PENALTY=0.213506
PRIMER_RIGHT_29_PENALTY=0.345764
PRIMER_LEFT_29_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_29_SEQUENCE=TGGTTCATGCTGATTCGTTC
PRIMER_LEFT_29=844,20
PRIMER_RIGHT_29=1738,20
PRIMER_LEFT_29_TM=60.214
PRIMER_RIGHT_29_TM=59.654
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=8.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=895
PRIMER_PAIR_30_PENALTY=0.560125
PRIMER_LEFT_30_PENALTY=0.119809
PRIMER_RIGHT_30_PENALTY=0.440316
PRIMER_LEFT_30_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_30_SEQUENCE=GCAAGCCGTGAAAGAAGTGT
PRIMER_LEFT_30=792,20
PRIMER_RIGHT_30=1611,20
PRIMER_LEFT_30_TM=60.120
PRIMER_RIGHT_30_TM=60.440
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=6.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=820
PRIMER_PAIR_31_PENALTY=0.579743
PRIMER_LEFT_31_PENALTY=0.213506
PRIMER_RIGHT_31_PENALTY=0.366237
PRIMER_LEFT_31_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_31_SEQUENCE=CGAAGAACTGTGCACGAAAG
PRIMER_LEFT_31=844,20
PRIMER_RIGHT_31=1632,20
PRIMER_LEFT_31_TM=60.214
PRIMER_RIGHT_31_TM=59.634
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=789
PRIMER_PAIR_32_PENALTY=0.592464
PRIMER_LEFT_32_PENALTY=0.213506
PRIMER_RIGHT_32_PENALTY=0.378958
PRIMER_LEFT_32_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_32_SEQUENCE=GAGCAAGCCGTGAAAGAAGT
PRIMER_LEFT_32=844,20
PRIMER_RIGHT_32=1613,20
PRIMER_LEFT_32_TM=60.214
PRIMER_RIGHT_32_TM=59.621
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=770
PRIMER_PAIR_33_PENALTY=0.642899
PRIMER_LEFT_33_PENALTY=0.071684
PRIMER_RIGHT_33_PENALTY=0.571215
PRIMER_LEFT_33_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_33_SEQUENCE=AGCAAGCCGTGAAAGAAGTG
PRIMER_LEFT_33=645,20
PRIMER_RIGHT_33=1612,20
PRIMER_LEFT_33_TM=59.928
PRIMER_RIGHT_33_TM=60.571
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=12.9000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=968
PRIMER_PAIR_34_PENALTY=0.644829
PRIMER_LEFT_34_PENALTY=0.073614
PRIMER_RIGHT_34_PENALTY=0.571215
PRIMER_LEFT_34_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_34_SEQUENCE=AGCAAGCCGTGAAAGAAGTG
PRIMER_LEFT_34=847,20
PRIMER_RIGHT_34=1612,20
PRIMER_LEFT_34_TM=60.074
PRIMER_RIGHT_34_TM=60.571
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=766
PRIMER_PAIR_35_PENALTY=0.645955
PRIMER_LEFT_35_PENALTY=0.071684
PRIMER_RIGHT_35_PENALTY=0.574272
PRIMER_LEFT_35_SEQUENCE=TGCTTATAACAATTCCCGCC
PRIMER_RIGHT_35_SEQUENCE=TCGAAGAACTGTGCACGAAA
PRIMER_LEFT_35=645,20
PRIMER_RIGHT_35=1633,20
PRIMER_LEFT_35_TM=59.928
PRIMER_RIGHT_35_TM=60.574
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=12.9000
PRIMER_RIGHT_35_END_STABILITY=9.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=989
PRIMER_PAIR_36_PENALTY=0.647886
PRIMER_LEFT_36_PENALTY=0.073614
PRIMER_RIGHT_36_PENALTY=0.574272
PRIMER_LEFT_36_SEQUENCE=GACGGTTTAGATTGCTCCCA
PRIMER_RIGHT_36_SEQUENCE=TCGAAGAACTGTGCACGAAA
PRIMER_LEFT_36=847,20
PRIMER_RIGHT_36=1633,20
PRIMER_LEFT_36_TM=60.074
PRIMER_RIGHT_36_TM=60.574
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=9.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=787
PRIMER_PAIR_37_PENALTY=0.653822
PRIMER_LEFT_37_PENALTY=0.213506
PRIMER_RIGHT_37_PENALTY=0.440316
PRIMER_LEFT_37_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_37_SEQUENCE=GCAAGCCGTGAAAGAAGTGT
PRIMER_LEFT_37=844,20
PRIMER_RIGHT_37=1611,20
PRIMER_LEFT_37_TM=60.214
PRIMER_RIGHT_37_TM=60.440
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.1000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=768
PRIMER_PAIR_38_PENALTY=0.691024
PRIMER_LEFT_38_PENALTY=0.119809
PRIMER_RIGHT_38_PENALTY=0.571215
PRIMER_LEFT_38_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_38_SEQUENCE=AGCAAGCCGTGAAAGAAGTG
PRIMER_LEFT_38=792,20
PRIMER_RIGHT_38=1612,20
PRIMER_LEFT_38_TM=60.120
PRIMER_RIGHT_38_TM=60.571
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=821
PRIMER_PAIR_39_PENALTY=0.694081
PRIMER_LEFT_39_PENALTY=0.119809
PRIMER_RIGHT_39_PENALTY=0.574272
PRIMER_LEFT_39_SEQUENCE=GCTGACTCTGGTCATTGGGT
PRIMER_RIGHT_39_SEQUENCE=TCGAAGAACTGTGCACGAAA
PRIMER_LEFT_39=792,20
PRIMER_RIGHT_39=1633,20
PRIMER_LEFT_39_TM=60.120
PRIMER_RIGHT_39_TM=60.574
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=9.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=842
PRIMER_PAIR_40_PENALTY=0.775863
PRIMER_LEFT_40_PENALTY=0.643602
PRIMER_RIGHT_40_PENALTY=0.132262
PRIMER_LEFT_40_SEQUENCE=GCTTATAACAATTCCCGCCC
PRIMER_RIGHT_40_SEQUENCE=AAGAGCAAGCCGTGAAAGAA
PRIMER_LEFT_40=646,20
PRIMER_RIGHT_40=1615,20
PRIMER_LEFT_40_TM=60.644
PRIMER_RIGHT_40_TM=60.132
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=12.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=970
PRIMER_PAIR_41_PENALTY=0.775863
PRIMER_LEFT_41_PENALTY=0.643602
PRIMER_RIGHT_41_PENALTY=0.132262
PRIMER_LEFT_41_SEQUENCE=GCTTATAACAATTCCCGCCC
PRIMER_RIGHT_41_SEQUENCE=AAAGAGCAAGCCGTGAAAGA
PRIMER_LEFT_41=646,20
PRIMER_RIGHT_41=1616,20
PRIMER_LEFT_41_TM=60.644
PRIMER_RIGHT_41_TM=60.132
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=12.9000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=971
PRIMER_PAIR_42_PENALTY=0.775863
PRIMER_LEFT_42_PENALTY=0.643602
PRIMER_RIGHT_42_PENALTY=0.132262
PRIMER_LEFT_42_SEQUENCE=GCTTATAACAATTCCCGCCC
PRIMER_RIGHT_42_SEQUENCE=GAAAGAGCAAGCCGTGAAAG
PRIMER_LEFT_42=646,20
PRIMER_RIGHT_42=1617,20
PRIMER_LEFT_42_TM=60.644
PRIMER_RIGHT_42_TM=60.132
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=12.9000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=972
PRIMER_PAIR_43_PENALTY=0.784721
PRIMER_LEFT_43_PENALTY=0.213506
PRIMER_RIGHT_43_PENALTY=0.571215
PRIMER_LEFT_43_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_43_SEQUENCE=AGCAAGCCGTGAAAGAAGTG
PRIMER_LEFT_43=844,20
PRIMER_RIGHT_43=1612,20
PRIMER_LEFT_43_TM=60.214
PRIMER_RIGHT_43_TM=60.571
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=769
PRIMER_PAIR_44_PENALTY=0.787777
PRIMER_LEFT_44_PENALTY=0.213506
PRIMER_RIGHT_44_PENALTY=0.574272
PRIMER_LEFT_44_SEQUENCE=TCCGACGGTTTAGATTGCTC
PRIMER_RIGHT_44_SEQUENCE=TCGAAGAACTGTGCACGAAA
PRIMER_LEFT_44=844,20
PRIMER_RIGHT_44=1633,20
PRIMER_LEFT_44_TM=60.214
PRIMER_RIGHT_44_TM=60.574
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=790
PRIMER_PAIR_45_PENALTY=0.886343
PRIMER_LEFT_45_PENALTY=0.643602
PRIMER_RIGHT_45_PENALTY=0.242742
PRIMER_LEFT_45_SEQUENCE=GCTTATAACAATTCCCGCCC
PRIMER_RIGHT_45_SEQUENCE=AGAGCAAGCCGTGAAAGAAG
PRIMER_LEFT_45=646,20
PRIMER_RIGHT_45=1614,20
PRIMER_LEFT_45_TM=60.644
PRIMER_RIGHT_45_TM=59.757
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=12.9000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=969
PRIMER_PAIR_46_PENALTY=1.003970
PRIMER_LEFT_46_PENALTY=0.643602
PRIMER_RIGHT_46_PENALTY=0.360369
PRIMER_LEFT_46_SEQUENCE=GCTTATAACAATTCCCGCCC
PRIMER_RIGHT_46_SEQUENCE=AACTGTGCACGAAAGAGCAA
PRIMER_LEFT_46=646,20
PRIMER_RIGHT_46=1627,20
PRIMER_LEFT_46_TM=60.644
PRIMER_RIGHT_46_TM=59.640
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=12.9000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=982
PRIMER_PAIR_47_PENALTY=1.009839
PRIMER_LEFT_47_PENALTY=0.643602
PRIMER_RIGHT_47_PENALTY=0.366237
PRIMER_LEFT_47_SEQUENCE=GCTTATAACAATTCCCGCCC
PRIMER_RIGHT_47_SEQUENCE=CGAAGAACTGTGCACGAAAG
PRIMER_LEFT_47=646,20
PRIMER_RIGHT_47=1632,20
PRIMER_LEFT_47_TM=60.644
PRIMER_RIGHT_47_TM=59.634
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=12.9000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=987
PRIMER_PAIR_48_PENALTY=1.022560
PRIMER_LEFT_48_PENALTY=0.643602
PRIMER_RIGHT_48_PENALTY=0.378958
PRIMER_LEFT_48_SEQUENCE=GCTTATAACAATTCCCGCCC
PRIMER_RIGHT_48_SEQUENCE=GAGCAAGCCGTGAAAGAAGT
PRIMER_LEFT_48=646,20
PRIMER_RIGHT_48=1613,20
PRIMER_LEFT_48_TM=60.644
PRIMER_RIGHT_48_TM=59.621
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=12.9000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=968
PRIMER_PAIR_49_PENALTY=1.058600
PRIMER_LEFT_49_PENALTY=0.926338
PRIMER_RIGHT_49_PENALTY=0.132262
PRIMER_LEFT_49_SEQUENCE=TGCTCCCATCTCTCACTCTG
PRIMER_RIGHT_49_SEQUENCE=AAGAGCAAGCCGTGAAAGAA
PRIMER_LEFT_49=859,20
PRIMER_RIGHT_49=1615,20
PRIMER_LEFT_49_TM=59.074
PRIMER_RIGHT_49_TM=60.132
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=757
=
SEQUENCE_ID=MAGIC.338_4_4529453_4529465_sf-2_kn-0_9656_22182_TRUE
SEQUENCE_TARGET=950,112
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_21556521_21556784_hi-0_ct-1_69373_159740_TRUE
SEQUENCE_TARGET=950,363
SEQUENCE_TEMPLATE=TTCCAAATTACACCTAAGAGGCGTAATATACAAAATGAGAAAAATATTGTTTTCTTAATAGACGAGTAATGTATGTGTATAAGCATGAACCGTAAGGATATATATTATCCACCAACACAAAANTTGAGTGATATATACATGATATATTAATACACAAGCATATAATAAGTAAATAAATTTATGTATAGCATGCTTGCATNGCGTTTCAAATAAATACAACATTTGCATATACATAAGTTCCTTCAAATATAAAACTTACTATAATTAGNCTAGAAACGAAAGTGTTAAGCTTCAAAAAATTACTTTATGTCTATTCTGATTTTAACGTACCTTTAGGGTTTGGATGTACCCTCGAATAAAAACTTAGGAATACAGTAAAAAAATATCTATATATACTTTGTACTTTTGTAGGATAATTGTTTATTTNTGAAGGGCTAATAGTTTGGCCACTAAAATTTATTTNNNTTTTTTTTTCCATATTTAAGTGAATCCACAACTTTAAATANCTTAAATAAAATTTGTTTGTTAATTATAAACGTGAAAACAGAAGAAAGTATTTGTGTTTNAAAAAAATAATTTGGCTGAATATTACATTTTCCTATTTCTATCTGTTTTAGCTATCGTAATGACTANTGAGCATATGATCCTTTTTGCACATATGATNTTATAAGAAAAAATATNTTTGCAATTTCACTTATGTAATTGTAAAAAGGGAAGTTTTGGTTAAATAATGCAGTTTTCTGTTCNTATGTGTGTTATGATCATTTTTGACATATGTTTTTTTAACGATAACATGAACACACGATCGAAAATGGACGTTTTTGAAACAAATTTGATACTCTAAAATATTCGGTGGCTTCAAACTTTAATTAAGTATCATGAGATAATATAGGTGGGTCTATATACACGCAGAAGCAAAAAGAGAAAGAAAAAAAAAATGAAAAATGAAAAACCAATCATCCAGAATTCGGAAAGGAAACACATGCTAATAAACACGAGGNAAAATGCGGCTAGGGAAAGACGAGTTTGTAACTACTAAGATGAAACTAATGTACGTGTGTGAATACATGATTCGTAGAAGTTTACGTCTGAGTTCTAAACACTTCATGAAGTGTCAAAGAGAACTAATATTCAAAATTGATAGTGATTAGCCTGAGAAACTAGGAGTCTCTCAACATTTCAATTTTGAGTTTTCTATATCAGTGTTATCAGACTTTGGCTAATTTTTAGGCTAATACAATTTTGTTTGTTCATAAAAAAAATNGTTTTCTGCATGTCGNAAAAAAACATTTCTACATCAAATGCAACATCACCACATGTATATTACAGATTACATATTTGCATATTGTATGAAATATCAATGTTTCTGATGGAGCTGCATAAGAGATAACACAATGAAGATATACTATACAGAATAATTCTATCAACTAAAATATTTTTTATTTTTATAACGAATTGTTTCTAATCGATGAAAAAAAGAAAACTAATGTATATGAACACAAATTAAGAGGNTGCTTTCCAATGGGTCTATGTAATTGCAATGCATGCTGTCTAGTTATTATCTCTTNGATAGTAAAATGAAANNNNNNNCNCCCCACTCTACTTGCGCTTGCGACCTATATATAGGCCTATAACCNTACTACAATTAGCGCTATCAATCAGTGAATTTACACCACTTTTCATCAAATCATTAATTTCACTTTCTAAAATTAANATTGAGTGGGTAAAAAAACAAATAAACCTAGGGTGAAACAAATTGAACTTTTTATTTATAAGGCTATTATTTATTTTTCTTTATATAACTATTCTGGGTTATGAAATCGATTTAATGAAGAAANTTTTTAAAATTATGAGATGTTAATTGATTTTTCTTTGAAATGGTTATAACTAATATACCAAAATATAATTAAATCGGATTATTATTCGTTTGACTCNNNNNNNNNNNNATGATTTTCACTTAAGACTGATCGACGAGTNCATCCTACTTGGATCATCATGTTTGGGTCAACAAACCATGCTTTGATATCATGTGACAAACNTAATTTTTTTGATTCTAAANGATTTGATAATACATAGATTAGTCTATACATCCATTATACATTANTTTATATATTNTAATTTAAAATATGGATCATATACAAACGTATCCCATTTAACAATTTCTAAACACATTTAGTTTATTTTTAAAAATTGTTTATAAATCGTATATACACAAGANTGGATGTAAAATCACATAATAACACCAATAATACTAATGCAAATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.187778
PRIMER_LEFT_0_PENALTY=0.658076
PRIMER_RIGHT_0_PENALTY=0.529702
PRIMER_LEFT_0_SEQUENCE=CACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_0_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_0=905,20
PRIMER_RIGHT_0=1622,20
PRIMER_LEFT_0_TM=59.342
PRIMER_RIGHT_0_TM=59.470
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=10.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=718
PRIMER_PAIR_1_PENALTY=1.398350
PRIMER_LEFT_1_PENALTY=0.658076
PRIMER_RIGHT_1_PENALTY=0.740274
PRIMER_LEFT_1_SEQUENCE=CACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_1_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_1=905,20
PRIMER_RIGHT_1=1615,20
PRIMER_LEFT_1_TM=59.342
PRIMER_RIGHT_1_TM=60.740
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=711
PRIMER_PAIR_2_PENALTY=1.553914
PRIMER_LEFT_2_PENALTY=1.024212
PRIMER_RIGHT_2_PENALTY=0.529702
PRIMER_LEFT_2_SEQUENCE=AACACACGATCGAAAATGGA
PRIMER_RIGHT_2_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_2=796,20
PRIMER_RIGHT_2=1622,20
PRIMER_LEFT_2_TM=58.976
PRIMER_RIGHT_2_TM=59.470
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=10.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=827
PRIMER_PAIR_3_PENALTY=1.553914
PRIMER_LEFT_3_PENALTY=1.024212
PRIMER_RIGHT_3_PENALTY=0.529702
PRIMER_LEFT_3_SEQUENCE=GAACACACGATCGAAAATGG
PRIMER_RIGHT_3_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_3=795,20
PRIMER_RIGHT_3=1622,20
PRIMER_LEFT_3_TM=58.976
PRIMER_RIGHT_3_TM=59.470
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=10.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=828
PRIMER_PAIR_4_PENALTY=1.582944
PRIMER_LEFT_4_PENALTY=0.658076
PRIMER_RIGHT_4_PENALTY=0.924868
PRIMER_LEFT_4_SEQUENCE=CACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_4_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_4=905,20
PRIMER_RIGHT_4=1620,20
PRIMER_LEFT_4_TM=59.342
PRIMER_RIGHT_4_TM=60.925
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=716
PRIMER_PAIR_5_PENALTY=1.678700
PRIMER_LEFT_5_PENALTY=1.148998
PRIMER_RIGHT_5_PENALTY=0.529702
PRIMER_LEFT_5_SEQUENCE=AACACACGATCGAAAATGGAC
PRIMER_RIGHT_5_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_5=796,21
PRIMER_RIGHT_5=1622,20
PRIMER_LEFT_5_TM=59.851
PRIMER_RIGHT_5_TM=59.470
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=10.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=827
PRIMER_PAIR_6_PENALTY=1.712360
PRIMER_LEFT_6_PENALTY=1.182658
PRIMER_RIGHT_6_PENALTY=0.529702
PRIMER_LEFT_6_SEQUENCE=ACACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_6_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_6=904,21
PRIMER_RIGHT_6=1622,20
PRIMER_LEFT_6_TM=60.183
PRIMER_RIGHT_6_TM=59.470
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=10.5000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=719
PRIMER_PAIR_7_PENALTY=1.764486
PRIMER_LEFT_7_PENALTY=1.024212
PRIMER_RIGHT_7_PENALTY=0.740274
PRIMER_LEFT_7_SEQUENCE=AACACACGATCGAAAATGGA
PRIMER_RIGHT_7_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_7=796,20
PRIMER_RIGHT_7=1615,20
PRIMER_LEFT_7_TM=58.976
PRIMER_RIGHT_7_TM=60.740
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=6.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=820
PRIMER_PAIR_8_PENALTY=1.764486
PRIMER_LEFT_8_PENALTY=1.024212
PRIMER_RIGHT_8_PENALTY=0.740274
PRIMER_LEFT_8_SEQUENCE=GAACACACGATCGAAAATGG
PRIMER_RIGHT_8_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_8=795,20
PRIMER_RIGHT_8=1615,20
PRIMER_LEFT_8_TM=58.976
PRIMER_RIGHT_8_TM=60.740
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=6.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=821
PRIMER_PAIR_9_PENALTY=1.889272
PRIMER_LEFT_9_PENALTY=1.148998
PRIMER_RIGHT_9_PENALTY=0.740274
PRIMER_LEFT_9_SEQUENCE=AACACACGATCGAAAATGGAC
PRIMER_RIGHT_9_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_9=796,21
PRIMER_RIGHT_9=1615,20
PRIMER_LEFT_9_TM=59.851
PRIMER_RIGHT_9_TM=60.740
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=820
PRIMER_PAIR_10_PENALTY=1.906933
PRIMER_LEFT_10_PENALTY=0.658076
PRIMER_RIGHT_10_PENALTY=1.248857
PRIMER_LEFT_10_SEQUENCE=CACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_10_SEQUENCE=ATATATAGGTCGCAAGCGCAA
PRIMER_LEFT_10=905,20
PRIMER_RIGHT_10=1623,21
PRIMER_LEFT_10_TM=59.342
PRIMER_RIGHT_10_TM=59.751
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=10.5000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=719
PRIMER_PAIR_11_PENALTY=1.922932
PRIMER_LEFT_11_PENALTY=1.182658
PRIMER_RIGHT_11_PENALTY=0.740274
PRIMER_LEFT_11_SEQUENCE=ACACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_11_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_11=904,21
PRIMER_RIGHT_11=1615,20
PRIMER_LEFT_11_TM=60.183
PRIMER_RIGHT_11_TM=60.740
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.1000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=712
PRIMER_PAIR_12_PENALTY=1.949080
PRIMER_LEFT_12_PENALTY=1.024212
PRIMER_RIGHT_12_PENALTY=0.924868
PRIMER_LEFT_12_SEQUENCE=AACACACGATCGAAAATGGA
PRIMER_RIGHT_12_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_12=796,20
PRIMER_RIGHT_12=1620,20
PRIMER_LEFT_12_TM=58.976
PRIMER_RIGHT_12_TM=60.925
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=825
PRIMER_PAIR_13_PENALTY=1.949080
PRIMER_LEFT_13_PENALTY=1.024212
PRIMER_RIGHT_13_PENALTY=0.924868
PRIMER_LEFT_13_SEQUENCE=GAACACACGATCGAAAATGG
PRIMER_RIGHT_13_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_13=795,20
PRIMER_RIGHT_13=1620,20
PRIMER_LEFT_13_TM=58.976
PRIMER_RIGHT_13_TM=60.925
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=826
PRIMER_PAIR_14_PENALTY=2.073866
PRIMER_LEFT_14_PENALTY=1.148998
PRIMER_RIGHT_14_PENALTY=0.924868
PRIMER_LEFT_14_SEQUENCE=AACACACGATCGAAAATGGAC
PRIMER_RIGHT_14_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_14=796,21
PRIMER_RIGHT_14=1620,20
PRIMER_LEFT_14_TM=59.851
PRIMER_RIGHT_14_TM=60.925
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=825
PRIMER_PAIR_15_PENALTY=2.085884
PRIMER_LEFT_15_PENALTY=0.658076
PRIMER_RIGHT_15_PENALTY=1.427808
PRIMER_LEFT_15_SEQUENCE=CACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_15_SEQUENCE=CATAGACCCATTGGAAAGCA
PRIMER_LEFT_15=905,20
PRIMER_RIGHT_15=1531,20
PRIMER_LEFT_15_TM=59.342
PRIMER_RIGHT_15_TM=58.572
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=627
PRIMER_PAIR_16_PENALTY=2.091575
PRIMER_LEFT_16_PENALTY=1.561873
PRIMER_RIGHT_16_PENALTY=0.529702
PRIMER_LEFT_16_SEQUENCE=CGCAGAAGCAAAAAGAGAAA
PRIMER_RIGHT_16_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_16=907,20
PRIMER_RIGHT_16=1622,20
PRIMER_LEFT_16_TM=58.438
PRIMER_RIGHT_16_TM=59.470
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=10.5000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=716
PRIMER_PAIR_17_PENALTY=2.107526
PRIMER_LEFT_17_PENALTY=1.182658
PRIMER_RIGHT_17_PENALTY=0.924868
PRIMER_LEFT_17_SEQUENCE=ACACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_17_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_17=904,21
PRIMER_RIGHT_17=1620,20
PRIMER_LEFT_17_TM=60.183
PRIMER_RIGHT_17_TM=60.925
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=717
PRIMER_PAIR_18_PENALTY=2.113855
PRIMER_LEFT_18_PENALTY=1.584153
PRIMER_RIGHT_18_PENALTY=0.529702
PRIMER_LEFT_18_SEQUENCE=ACACACGATCGAAAATGGAC
PRIMER_RIGHT_18_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_18=797,20
PRIMER_RIGHT_18=1622,20
PRIMER_LEFT_18_TM=58.416
PRIMER_RIGHT_18_TM=59.470
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=10.5000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=826
PRIMER_PAIR_19_PENALTY=2.118825
PRIMER_LEFT_19_PENALTY=1.589123
PRIMER_RIGHT_19_PENALTY=0.529702
PRIMER_LEFT_19_SEQUENCE=CGCAGAAGCAAAAAGAGAAAG
PRIMER_RIGHT_19_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_19=907,21
PRIMER_RIGHT_19=1622,20
PRIMER_LEFT_19_TM=59.411
PRIMER_RIGHT_19_TM=59.470
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=10.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=716
PRIMER_PAIR_20_PENALTY=2.203599
PRIMER_LEFT_20_PENALTY=1.673897
PRIMER_RIGHT_20_PENALTY=0.529702
PRIMER_LEFT_20_SEQUENCE=CACGCAGAAGCAAAAAGAGAA
PRIMER_RIGHT_20_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_20=905,21
PRIMER_RIGHT_20=1622,20
PRIMER_LEFT_20_TM=60.674
PRIMER_RIGHT_20_TM=59.470
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=10.5000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=718
PRIMER_PAIR_21_PENALTY=2.214845
PRIMER_LEFT_21_PENALTY=0.658076
PRIMER_RIGHT_21_PENALTY=1.556768
PRIMER_LEFT_21_SEQUENCE=CACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_21_SEQUENCE=ACATAGACCCATTGGAAAGCA
PRIMER_LEFT_21=905,20
PRIMER_RIGHT_21=1532,21
PRIMER_LEFT_21_TM=59.342
PRIMER_RIGHT_21_TM=59.443
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=628
PRIMER_PAIR_22_PENALTY=2.219933
PRIMER_LEFT_22_PENALTY=1.690231
PRIMER_RIGHT_22_PENALTY=0.529702
PRIMER_LEFT_22_SEQUENCE=ACACGCAGAAGCAAAAAGAG
PRIMER_RIGHT_22_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_22=904,20
PRIMER_RIGHT_22=1622,20
PRIMER_LEFT_22_TM=58.310
PRIMER_RIGHT_22_TM=59.470
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=10.5000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=719
PRIMER_PAIR_23_PENALTY=2.258114
PRIMER_LEFT_23_PENALTY=0.658076
PRIMER_RIGHT_23_PENALTY=1.600038
PRIMER_LEFT_23_SEQUENCE=CACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_23_SEQUENCE=TAGGTCGCAAGCGCAAGTAG
PRIMER_LEFT_23=905,20
PRIMER_RIGHT_23=1618,20
PRIMER_LEFT_23_TM=59.342
PRIMER_RIGHT_23_TM=61.600
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=5.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=714
PRIMER_PAIR_24_PENALTY=2.273069
PRIMER_LEFT_24_PENALTY=1.024212
PRIMER_RIGHT_24_PENALTY=1.248857
PRIMER_LEFT_24_SEQUENCE=AACACACGATCGAAAATGGA
PRIMER_RIGHT_24_SEQUENCE=ATATATAGGTCGCAAGCGCAA
PRIMER_LEFT_24=796,20
PRIMER_RIGHT_24=1623,21
PRIMER_LEFT_24_TM=58.976
PRIMER_RIGHT_24_TM=59.751
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=10.5000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=828
PRIMER_PAIR_25_PENALTY=2.273069
PRIMER_LEFT_25_PENALTY=1.024212
PRIMER_RIGHT_25_PENALTY=1.248857
PRIMER_LEFT_25_SEQUENCE=GAACACACGATCGAAAATGG
PRIMER_RIGHT_25_SEQUENCE=ATATATAGGTCGCAAGCGCAA
PRIMER_LEFT_25=795,20
PRIMER_RIGHT_25=1623,21
PRIMER_LEFT_25_TM=58.976
PRIMER_RIGHT_25_TM=59.751
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=10.5000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=829
PRIMER_PAIR_26_PENALTY=2.302147
PRIMER_LEFT_26_PENALTY=1.561873
PRIMER_RIGHT_26_PENALTY=0.740274
PRIMER_LEFT_26_SEQUENCE=CGCAGAAGCAAAAAGAGAAA
PRIMER_RIGHT_26_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_26=907,20
PRIMER_RIGHT_26=1615,20
PRIMER_LEFT_26_TM=58.438
PRIMER_RIGHT_26_TM=60.740
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=709
PRIMER_PAIR_27_PENALTY=2.324427
PRIMER_LEFT_27_PENALTY=1.584153
PRIMER_RIGHT_27_PENALTY=0.740274
PRIMER_LEFT_27_SEQUENCE=ACACACGATCGAAAATGGAC
PRIMER_RIGHT_27_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_27=797,20
PRIMER_RIGHT_27=1615,20
PRIMER_LEFT_27_TM=58.416
PRIMER_RIGHT_27_TM=60.740
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.1000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=819
PRIMER_PAIR_28_PENALTY=2.329397
PRIMER_LEFT_28_PENALTY=1.589123
PRIMER_RIGHT_28_PENALTY=0.740274
PRIMER_LEFT_28_SEQUENCE=CGCAGAAGCAAAAAGAGAAAG
PRIMER_RIGHT_28_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_28=907,21
PRIMER_RIGHT_28=1615,20
PRIMER_LEFT_28_TM=59.411
PRIMER_RIGHT_28_TM=60.740
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=709
PRIMER_PAIR_29_PENALTY=2.397855
PRIMER_LEFT_29_PENALTY=1.148998
PRIMER_RIGHT_29_PENALTY=1.248857
PRIMER_LEFT_29_SEQUENCE=AACACACGATCGAAAATGGAC
PRIMER_RIGHT_29_SEQUENCE=ATATATAGGTCGCAAGCGCAA
PRIMER_LEFT_29=796,21
PRIMER_RIGHT_29=1623,21
PRIMER_LEFT_29_TM=59.851
PRIMER_RIGHT_29_TM=59.751
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=10.5000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=828
PRIMER_PAIR_30_PENALTY=2.414171
PRIMER_LEFT_30_PENALTY=1.673897
PRIMER_RIGHT_30_PENALTY=0.740274
PRIMER_LEFT_30_SEQUENCE=CACGCAGAAGCAAAAAGAGAA
PRIMER_RIGHT_30_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_30=905,21
PRIMER_RIGHT_30=1615,20
PRIMER_LEFT_30_TM=60.674
PRIMER_RIGHT_30_TM=60.740
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=711
PRIMER_PAIR_31_PENALTY=2.418783
PRIMER_LEFT_31_PENALTY=1.889081
PRIMER_RIGHT_31_PENALTY=0.529702
PRIMER_LEFT_31_SEQUENCE=GAACACACGATCGAAAATGGA
PRIMER_RIGHT_31_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_31=795,21
PRIMER_RIGHT_31=1622,20
PRIMER_LEFT_31_TM=60.889
PRIMER_RIGHT_31_TM=59.470
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=10.5000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=828
PRIMER_PAIR_32_PENALTY=2.430450
PRIMER_LEFT_32_PENALTY=1.900748
PRIMER_RIGHT_32_PENALTY=0.529702
PRIMER_LEFT_32_SEQUENCE=CATGAACACACGATCGAAAA
PRIMER_RIGHT_32_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_32=792,20
PRIMER_RIGHT_32=1622,20
PRIMER_LEFT_32_TM=58.099
PRIMER_RIGHT_32_TM=59.470
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=10.5000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=831
PRIMER_PAIR_33_PENALTY=2.430505
PRIMER_LEFT_33_PENALTY=1.690231
PRIMER_RIGHT_33_PENALTY=0.740274
PRIMER_LEFT_33_SEQUENCE=ACACGCAGAAGCAAAAAGAG
PRIMER_RIGHT_33_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_33=904,20
PRIMER_RIGHT_33=1615,20
PRIMER_LEFT_33_TM=58.310
PRIMER_RIGHT_33_TM=60.740
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.1000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=712
PRIMER_PAIR_34_PENALTY=2.431515
PRIMER_LEFT_34_PENALTY=1.182658
PRIMER_RIGHT_34_PENALTY=1.248857
PRIMER_LEFT_34_SEQUENCE=ACACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_34_SEQUENCE=ATATATAGGTCGCAAGCGCAA
PRIMER_LEFT_34=904,21
PRIMER_RIGHT_34=1623,21
PRIMER_LEFT_34_TM=60.183
PRIMER_RIGHT_34_TM=59.751
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=10.5000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=720
PRIMER_PAIR_35_PENALTY=2.452019
PRIMER_LEFT_35_PENALTY=1.024212
PRIMER_RIGHT_35_PENALTY=1.427808
PRIMER_LEFT_35_SEQUENCE=AACACACGATCGAAAATGGA
PRIMER_RIGHT_35_SEQUENCE=CATAGACCCATTGGAAAGCA
PRIMER_LEFT_35=796,20
PRIMER_RIGHT_35=1531,20
PRIMER_LEFT_35_TM=58.976
PRIMER_RIGHT_35_TM=58.572
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=736
PRIMER_PAIR_36_PENALTY=2.452019
PRIMER_LEFT_36_PENALTY=1.024212
PRIMER_RIGHT_36_PENALTY=1.427808
PRIMER_LEFT_36_SEQUENCE=GAACACACGATCGAAAATGG
PRIMER_RIGHT_36_SEQUENCE=CATAGACCCATTGGAAAGCA
PRIMER_LEFT_36=795,20
PRIMER_RIGHT_36=1531,20
PRIMER_LEFT_36_TM=58.976
PRIMER_RIGHT_36_TM=58.572
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.4000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=737
PRIMER_PAIR_37_PENALTY=2.486740
PRIMER_LEFT_37_PENALTY=1.561873
PRIMER_RIGHT_37_PENALTY=0.924868
PRIMER_LEFT_37_SEQUENCE=CGCAGAAGCAAAAAGAGAAA
PRIMER_RIGHT_37_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_37=907,20
PRIMER_RIGHT_37=1620,20
PRIMER_LEFT_37_TM=58.438
PRIMER_RIGHT_37_TM=60.925
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=714
PRIMER_PAIR_38_PENALTY=2.509021
PRIMER_LEFT_38_PENALTY=1.584153
PRIMER_RIGHT_38_PENALTY=0.924868
PRIMER_LEFT_38_SEQUENCE=ACACACGATCGAAAATGGAC
PRIMER_RIGHT_38_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_38=797,20
PRIMER_RIGHT_38=1620,20
PRIMER_LEFT_38_TM=58.416
PRIMER_RIGHT_38_TM=60.925
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=824
PRIMER_PAIR_39_PENALTY=2.513991
PRIMER_LEFT_39_PENALTY=1.589123
PRIMER_RIGHT_39_PENALTY=0.924868
PRIMER_LEFT_39_SEQUENCE=CGCAGAAGCAAAAAGAGAAAG
PRIMER_RIGHT_39_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_39=907,21
PRIMER_RIGHT_39=1620,20
PRIMER_LEFT_39_TM=59.411
PRIMER_RIGHT_39_TM=60.925
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=714
PRIMER_PAIR_40_PENALTY=2.576806
PRIMER_LEFT_40_PENALTY=1.148998
PRIMER_RIGHT_40_PENALTY=1.427808
PRIMER_LEFT_40_SEQUENCE=AACACACGATCGAAAATGGAC
PRIMER_RIGHT_40_SEQUENCE=CATAGACCCATTGGAAAGCA
PRIMER_LEFT_40=796,21
PRIMER_RIGHT_40=1531,20
PRIMER_LEFT_40_TM=59.851
PRIMER_RIGHT_40_TM=58.572
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=736
PRIMER_PAIR_41_PENALTY=2.580980
PRIMER_LEFT_41_PENALTY=1.024212
PRIMER_RIGHT_41_PENALTY=1.556768
PRIMER_LEFT_41_SEQUENCE=AACACACGATCGAAAATGGA
PRIMER_RIGHT_41_SEQUENCE=ACATAGACCCATTGGAAAGCA
PRIMER_LEFT_41=796,20
PRIMER_RIGHT_41=1532,21
PRIMER_LEFT_41_TM=58.976
PRIMER_RIGHT_41_TM=59.443
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=737
PRIMER_PAIR_42_PENALTY=2.580980
PRIMER_LEFT_42_PENALTY=1.024212
PRIMER_RIGHT_42_PENALTY=1.556768
PRIMER_LEFT_42_SEQUENCE=GAACACACGATCGAAAATGG
PRIMER_RIGHT_42_SEQUENCE=ACATAGACCCATTGGAAAGCA
PRIMER_LEFT_42=795,20
PRIMER_RIGHT_42=1532,21
PRIMER_LEFT_42_TM=58.976
PRIMER_RIGHT_42_TM=59.443
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=738
PRIMER_PAIR_43_PENALTY=2.598765
PRIMER_LEFT_43_PENALTY=1.673897
PRIMER_RIGHT_43_PENALTY=0.924868
PRIMER_LEFT_43_SEQUENCE=CACGCAGAAGCAAAAAGAGAA
PRIMER_RIGHT_43_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_43=905,21
PRIMER_RIGHT_43=1620,20
PRIMER_LEFT_43_TM=60.674
PRIMER_RIGHT_43_TM=60.925
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=716
PRIMER_PAIR_44_PENALTY=2.610466
PRIMER_LEFT_44_PENALTY=1.182658
PRIMER_RIGHT_44_PENALTY=1.427808
PRIMER_LEFT_44_SEQUENCE=ACACGCAGAAGCAAAAAGAGA
PRIMER_RIGHT_44_SEQUENCE=CATAGACCCATTGGAAAGCA
PRIMER_LEFT_44=904,21
PRIMER_RIGHT_44=1531,20
PRIMER_LEFT_44_TM=60.183
PRIMER_RIGHT_44_TM=58.572
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=628
PRIMER_PAIR_45_PENALTY=2.615098
PRIMER_LEFT_45_PENALTY=1.690231
PRIMER_RIGHT_45_PENALTY=0.924868
PRIMER_LEFT_45_SEQUENCE=ACACGCAGAAGCAAAAAGAG
PRIMER_RIGHT_45_SEQUENCE=TATAGGTCGCAAGCGCAAGT
PRIMER_LEFT_45=904,20
PRIMER_RIGHT_45=1620,20
PRIMER_LEFT_45_TM=58.310
PRIMER_RIGHT_45_TM=60.925
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=717
PRIMER_PAIR_46_PENALTY=2.623878
PRIMER_LEFT_46_PENALTY=2.094176
PRIMER_RIGHT_46_PENALTY=0.529702
PRIMER_LEFT_46_SEQUENCE=ACGCAGAAGCAAAAAGAGAA
PRIMER_RIGHT_46_SEQUENCE=TATATAGGTCGCAAGCGCAA
PRIMER_LEFT_46=906,20
PRIMER_RIGHT_46=1622,20
PRIMER_LEFT_46_TM=57.906
PRIMER_RIGHT_46_TM=59.470
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=10.5000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=717
PRIMER_PAIR_47_PENALTY=2.624249
PRIMER_LEFT_47_PENALTY=1.024212
PRIMER_RIGHT_47_PENALTY=1.600038
PRIMER_LEFT_47_SEQUENCE=AACACACGATCGAAAATGGA
PRIMER_RIGHT_47_SEQUENCE=TAGGTCGCAAGCGCAAGTAG
PRIMER_LEFT_47=796,20
PRIMER_RIGHT_47=1618,20
PRIMER_LEFT_47_TM=58.976
PRIMER_RIGHT_47_TM=61.600
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=5.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=823
PRIMER_PAIR_48_PENALTY=2.624249
PRIMER_LEFT_48_PENALTY=1.024212
PRIMER_RIGHT_48_PENALTY=1.600038
PRIMER_LEFT_48_SEQUENCE=GAACACACGATCGAAAATGG
PRIMER_RIGHT_48_SEQUENCE=TAGGTCGCAAGCGCAAGTAG
PRIMER_LEFT_48=795,20
PRIMER_RIGHT_48=1618,20
PRIMER_LEFT_48_TM=58.976
PRIMER_RIGHT_48_TM=61.600
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=5.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=824
PRIMER_PAIR_49_PENALTY=2.629355
PRIMER_LEFT_49_PENALTY=1.889081
PRIMER_RIGHT_49_PENALTY=0.740274
PRIMER_LEFT_49_SEQUENCE=GAACACACGATCGAAAATGGA
PRIMER_RIGHT_49_SEQUENCE=GTCGCAAGCGCAAGTAGAGT
PRIMER_LEFT_49=795,21
PRIMER_RIGHT_49=1615,20
PRIMER_LEFT_49_TM=60.889
PRIMER_RIGHT_49_TM=60.740
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=6.1000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=821
=
SEQUENCE_ID=MAGIC.426_5_19591917_19592642_sf-2_po-0_925616_255404_TRUE
SEQUENCE_TARGET=950,825
SEQUENCE_TEMPLATE=TCAGAAGCTAGGCTTTGGCTTTGTAGTGCTCTTTCCACTATTTCTATATCTCCTAGNAAGCAACTTAATATCTTCATGAAGTTATTGAGATCAAAACCTAGAAAGATGCAGTTNTTATCTCAGNTTTTGACNATGATGTTTGAGAAGAGACCAAGGAAACTTGGATNTTTGNTAGCTAAAAGAAGTTATATACTTGAGAAGTTCTTTGAAGGTAAAAGAGTCTTTGGTTTCACATTGTGTATTTACATTATCTTAAGATTGTGCTTGATGTTNNGTTTTTGATATTTGTAGGGAATCAGAAGCGTATACTAGAGTGGTTTTCTGAGTTTGCTTATGATGGTGGNTCTGATCACAAGAGAGGTGCAAAAGCATTGGCGCAGTTTGCGTTTGCGAATCGGGATATTTGTTGGGAAGAGCTTGAGTGGCGAGGTAAACANGGGCAATCGCCTGCNGTTGTAGCTACAAAGCCTCATTATCTTCTTGATTTAGACGTTGAAAGAACTATTNAGAATTTTCTTGACAATGTCCCTGAGTTCTGGTCTTCCAATGAGTTTGCTGAGTCGCTTAAAGATGGACAGATTTTGTTTCTTGANACCAAATTCTTTATAGATCTCTTTATTCGTNTCATGTATGAAGAAGATATGTATGATGTGTGGGATGNTGTTGAGGAGTTTCTTAGGGAGGAGTCTTTCTCTTCNTTAACACAGCATCTCCTTATCACTCTTGAAGAACGTGACTTGTGTCGNTTTNTTGAATTGCTTGGTAACTATTTTGAGCCANGTATTGAATCTTGGGATAGTGGTGANTCGTCTNGCTGGCTTGGGGTTNTTCTTTCAAGATATGTTGACACAGAATCTATCGACGAGCTNCTGCTGCTGAGTTCTATTATCAATCAAGGTCGTCAGTTGNTGCGGCTTGTACGTGATGAAAACGGGAATNNTGAGGGAGAACTGTTGAAAGAAACNATGGCAGAGATATGCAGAGGTTTGNAAAACGNNNNNAGCTNTTCTNTNATNCTGAGAGAGCTNTNGAAGATGAAACACATANAGGTGATAAAATTACTCGGGCTACTTTCTTGGACTATTCACTTCAGACTATCAGAGGAATGTCAGACNCCAGATTCTTGGGAGCTTCTATTTNNAGAAAATGGGATTGAGTTCCGCAGGTCTAGTGACNATTCGTTGCTAAGCTATAATGGANTNTCTGAAGAAANTGAATCTGACTCAGATAGTAGAAGCCGTGTNTCTAANAAGAGGCACAAGAGGGAGAAGAANAAAAGAAAGAAGAAAAAGAAGAGGGCTTTNNNTGATGATGATGATCNTGGTGATGATGAGCTTTTAGNTCTGCATTCAATAAGTCGAAGTTGGTTGCTATNTACTGATGGGTTNTCTGCAACATGGACCAGTGTGANNNNNNNNNNNNNNNNNNNNNNNNTTNCGTATANTNNATGTNTTTCTTATTTGTGTTGTNTTANNATCTGAGATNGNTTNTTTCAGGTGGATTTACCGGAATACATCGCAAAGNATTGTTTGTCGACATGGATGAAGNGGTTATTAGCTAGACAAAAATGATTCACANACAAGNNNNNNNTCAGATGACAAAATAGTAAGGTATTGGTNACAACCTCAACTTGNACGTTTTCTTGTACTAAANAAAAGNGATTTCAAAGATGNNNNNATCAACCTAATGGTTNCTACTTGCTCTTCTTTACAGATCCTACCANNANGTACAANGTGCNAAACTCGAANATATTCACCATNNATAGGNATAACATATGNTCANGAAGGTGTNGAAGCAATGNAAGAGAAGAGGNATGANACTGAATCAGTCATNATCACTCTATGTNNANNNTGTANNGTTTTTGGCTGGATTAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACCCTTTATTGCTTCTGATNNTCAGAACTAATGTANAGTANNCTNTTGCATTGTNTAGCTTTTGATAGTGAAAAATATTTTTGAATANAAAGCTTATCTACTGTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACAGTAGCTGTANCGTGNTTAAAGCCTCGTGAAACCAAAAGGTCGACATGCATTTAAGTTTNGTCTCTTCGTTTTCCTCTAGAAAGGTTGATGGTAACAANNATTNATGCCTCGTTAATCTAAAAGTTGGAGACAGCTNNNATCATCATACTTTCGACTCNTTGTAGTCCTCTCTAANGGTTGTGACTTTTGCAATTCCCTTTCATGTCTATATAAAGACACGTATGTTCAGAATGTTATCTANCACTTACCCTCNTTATTTCAGNGATGTCTTCTTCCATTGTTAAAATCGGAATAGATANCATCAAAACAACGGTAACTGAAAAAGAGCGAGATATCAACC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_12160869_12161462_wil-2_po-0_7830_93283_TRUE
SEQUENCE_TARGET=950,693
SEQUENCE_TEMPLATE=TCCATCGTATGCAAGGGTCGAGAGAATATTCTGGTCAGAGTATCTGAGCATACACTCATCATAATAAAACANNGATTTTCTCTCACTCGNACACTGAACGAGTGTGTCGTTGATGGAGAAGGCGACGCAGTTACGACAAACTNCCGGTGAGACGTCTCCGCGGCAAAGGAAAAGTCCGGTGACTCTATCAGGGGCTTGTCCCGCCCTGGCGCTTTGGAATCCAGTAGAGTAAGAGGCGTTGGGGGCAGAAAGAGAAGACAAAAGGGTTCTGAGATTAGTCAAGTAAGGGCTGTTTCTTGAATAAGTTGTCGTATTTGGGCAAATATGCTTTATATAGAAAACATTTTCAACAGAAGCTGTGAAGCTAGTGAGGAAGAAGAAAAGGAAGATGAAAGAGGCACCANAAGACATTGTTCGATTTCCTCAGCTTTTGTTTCAGATCATGAATCTCCTAATTATTATGAATATTTTCTTCTTCTCTCTGTTACGTGAAACAGAGGATTGACTACTACTATAAGTCAACTGCTTTTGTCTTTTTAAGTGGGAAAACAAGACAGGAAGAGTTNATAATGNNAGTGACCATTANATTTTTGGCTTATNANANNNNNNNCNTATNATCATTAACCTCTAANNTCANCGANGATNAACACTCGTGATNGATGCATCATNAATGGAACAAGCAAACGACATGGTAGTAGACGGACCAGGCTCNANTCTCTCGGCTAATGGGTTTGGCTCGGATCTGACCCTGAANACAAATCCAGGTAATTGAGGCACGGGCANAGTAAGAAAAGTATTAGTGAGCATNTGAAANACTGTGGACATNGTTGGNCGATCTGCAGGATTTNCTTGAACGCACAATAANCNTATATGGATGCATCTNATGNCTTCATCTTTATCATAANTCTCCCCCATGGCNGGGTCTACNAGTTCCAAGAATGATTCGTTGTTCCAAAGCCTCCAAACCTGNTAAGTNACAATAAACCAATGTTCNNTCNNNNATCAAAGTTTAAAGAGGTCAAAAATAAGTTCAAGATTGCATTGGCTTTGTAGGACTAACATATGTGACCAAGTTTCCTACTGAACCATCAATCTCATGGAAGCTACTACTCTTTTTGCCAACAATAATCTCCAGANTCANTACTCCNAAACTATAAACATCAGATTTCATCGAGAACTGCCCNTTCGCCACATANTCNGGAGGCATGTAACCGCTGCAAAACACAGCACATATCACTTTAAGTTTTATTAGGGAAAGAAAAGGGAATAGACAGAGTGTAATTGAAGTTAACAATNACTTACAATGTTCCAACTACTCTTCCTGTGGTAGCTTCAGTNTGGTCCACTCTNAAATTCCTAGCCACACCAAAATCCGCGATTTTCGGNTTCATATCNNCATCTAAGAGAATGTTACCCGCTTTGAGGTCACGGTGTATGATTGTAAGCCGTGAATCTTGATGGAGATATAAAATCCCTCGAGTCACTCCCCTGATTATGTTGTATCGTGTTCTCCAATCCAGNTGACCCCTCCTTCTATGGTCTGCACATCCAACGTATAGAGCNATTAGACTATGGGTAAGAGATCAAAAACAANTGTTTCAGAGAATCTAATCCTATATATCATATGNTTCTTACCAAAGAGNAANTAGTCAAGACTTTTATTTGGCANATACTCGTAGACAAGTATCTTTTCTTCTCCTTCAACAGAGAACCCNAGAAGTCTAACGAGATTTCTATGTTGGAGCTTTGCTACAAGAAGCACCTCGTTCTTNAACTCTTCTTCACCTTGACCTGAAATTTTNGACAGCCTCTTCACAGCAACTTCNGTTCCATTCGGNAANGTTCCCTGTCAATTCNTATGATNTTCACTNACTNTCAGAATTTCTTATNTTTTTTTTTGGCAACTAAGTTTATAACACAAACTAGTAGNTGAGAAAGTTTAAGAACATTNTGCCTTAAAAANTTCACCAAATCCACCATGACCAAGCTTGTTNCTCTTCTNAAAATTANTTGTTGCAGCTTCAATTGCTTTAAAATCGAATTGAAGTGAACCTGANGTTGTAATATCATCTGCAGCAGAAAGCATANATACATAGTGTTATGGTGGAAACGTATCCAAAGGGACATTAACAGTTTTAATGCAAATAGCTGTATAGTCTCTTNCCATCATCTGGNGGTGCAGTACCATAAGTNGTCATTGANCTCCCTGCAAGANAAATATAACCATCGTTGAAGAACAGAGAAAGAGACAACTGAATTGACATATTTAAAAACGTTTATGCCCTGAACTCATAATTGTTAAAANAAANAAAAAAACTCACTTTCAGTTGCAAACTCTTGGTAGGCTTTTCTCCTCCTANAAAGAGNATACCCTAGAACAAGCAGTACCGTGATAAAAAAGGCAAGAGCAACAGTGATTCCGAGGATATTTCTTGTAGAAATNTTTCCGCCATCTGAACAAGGCCAAATNACCTACGATTATTGCGCTTAAGGGAAAATATGAGACTANAAAGCTAGTACCGTCAGATAGTTCTGAGATTCTACCTTTTTCACGAGGAAGCGTGGGAGAAGCCCTATACTCAATAAATCCATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=5.308335
PRIMER_LEFT_0_PENALTY=1.054343
PRIMER_RIGHT_0_PENALTY=4.253992
PRIMER_LEFT_0_SEQUENCE=CAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_0_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_0=755,21
PRIMER_RIGHT_0=1753,22
PRIMER_LEFT_0_TM=59.946
PRIMER_RIGHT_0_TM=57.746
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=5.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=999
PRIMER_PAIR_1_PENALTY=5.352361
PRIMER_LEFT_1_PENALTY=1.054343
PRIMER_RIGHT_1_PENALTY=4.298018
PRIMER_LEFT_1_SEQUENCE=CAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_1_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_1=755,21
PRIMER_RIGHT_1=1749,20
PRIMER_LEFT_1_TM=59.946
PRIMER_RIGHT_1_TM=55.702
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=995
PRIMER_PAIR_2_PENALTY=5.767664
PRIMER_LEFT_2_PENALTY=1.054343
PRIMER_RIGHT_2_PENALTY=4.713321
PRIMER_LEFT_2_SEQUENCE=CAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_2_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_2=755,21
PRIMER_RIGHT_2=1750,20
PRIMER_LEFT_2_TM=59.946
PRIMER_RIGHT_2_TM=55.287
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=996
PRIMER_PAIR_3_PENALTY=6.222399
PRIMER_LEFT_3_PENALTY=1.054343
PRIMER_RIGHT_3_PENALTY=5.168056
PRIMER_LEFT_3_SEQUENCE=CAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_3_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_3=755,21
PRIMER_RIGHT_3=1753,21
PRIMER_LEFT_3_TM=59.946
PRIMER_RIGHT_3_TM=55.832
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=999
PRIMER_PAIR_4_PENALTY=6.628301
PRIMER_LEFT_4_PENALTY=1.054343
PRIMER_RIGHT_4_PENALTY=5.573958
PRIMER_LEFT_4_SEQUENCE=CAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_4_SEQUENCE=GTAGCAAAGCTCCAACATAGAA
PRIMER_LEFT_4=755,21
PRIMER_RIGHT_4=1752,22
PRIMER_LEFT_4_TM=59.946
PRIMER_RIGHT_4_TM=56.426
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=998
PRIMER_PAIR_5_PENALTY=6.975444
PRIMER_LEFT_5_PENALTY=2.721451
PRIMER_RIGHT_5_PENALTY=4.253992
PRIMER_LEFT_5_SEQUENCE=CAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_5_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_5=755,22
PRIMER_RIGHT_5=1753,22
PRIMER_LEFT_5_TM=60.721
PRIMER_RIGHT_5_TM=57.746
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=5.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=999
PRIMER_PAIR_6_PENALTY=6.975444
PRIMER_LEFT_6_PENALTY=2.721451
PRIMER_RIGHT_6_PENALTY=4.253992
PRIMER_LEFT_6_SEQUENCE=ACAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_6_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_6=754,22
PRIMER_RIGHT_6=1753,22
PRIMER_LEFT_6_TM=60.721
PRIMER_RIGHT_6_TM=57.746
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=5.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=1000
PRIMER_PAIR_7_PENALTY=7.019470
PRIMER_LEFT_7_PENALTY=2.721451
PRIMER_RIGHT_7_PENALTY=4.298018
PRIMER_LEFT_7_SEQUENCE=CAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_7_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_7=755,22
PRIMER_RIGHT_7=1749,20
PRIMER_LEFT_7_TM=60.721
PRIMER_RIGHT_7_TM=55.702
PRIMER_LEFT_7_GC_PERCENT=45.455
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=995
PRIMER_PAIR_8_PENALTY=7.019470
PRIMER_LEFT_8_PENALTY=2.721451
PRIMER_RIGHT_8_PENALTY=4.298018
PRIMER_LEFT_8_SEQUENCE=ACAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_8_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_8=754,22
PRIMER_RIGHT_8=1749,20
PRIMER_LEFT_8_TM=60.721
PRIMER_RIGHT_8_TM=55.702
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=996
PRIMER_PAIR_9_PENALTY=7.031126
PRIMER_LEFT_9_PENALTY=0.205704
PRIMER_RIGHT_9_PENALTY=6.825421
PRIMER_LEFT_9_SEQUENCE=TCTCTCGGCTAATGGGTTTG
PRIMER_RIGHT_9_SEQUENCE=CTCTGTTGAAGGAGAAGAAAAG
PRIMER_LEFT_9=714,20
PRIMER_RIGHT_9=1708,22
PRIMER_LEFT_9_TM=60.206
PRIMER_RIGHT_9_TM=55.175
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=995
PRIMER_PAIR_10_PENALTY=7.099081
PRIMER_LEFT_10_PENALTY=2.845089
PRIMER_RIGHT_10_PENALTY=4.253992
PRIMER_LEFT_10_SEQUENCE=AAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_10_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_10=756,20
PRIMER_RIGHT_10=1753,22
PRIMER_LEFT_10_TM=57.155
PRIMER_RIGHT_10_TM=57.746
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=5.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=998
PRIMER_PAIR_11_PENALTY=7.143107
PRIMER_LEFT_11_PENALTY=2.845089
PRIMER_RIGHT_11_PENALTY=4.298018
PRIMER_LEFT_11_SEQUENCE=AAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_11_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_11=756,20
PRIMER_RIGHT_11=1749,20
PRIMER_LEFT_11_TM=57.155
PRIMER_RIGHT_11_TM=55.702
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=994
PRIMER_PAIR_12_PENALTY=7.195479
PRIMER_LEFT_12_PENALTY=2.941487
PRIMER_RIGHT_12_PENALTY=4.253992
PRIMER_LEFT_12_SEQUENCE=AAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_12_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_12=756,21
PRIMER_RIGHT_12=1753,22
PRIMER_LEFT_12_TM=58.059
PRIMER_RIGHT_12_TM=57.746
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=5.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=998
PRIMER_PAIR_13_PENALTY=7.239505
PRIMER_LEFT_13_PENALTY=2.941487
PRIMER_RIGHT_13_PENALTY=4.298018
PRIMER_LEFT_13_SEQUENCE=AAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_13_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_13=756,21
PRIMER_RIGHT_13=1749,20
PRIMER_LEFT_13_TM=58.059
PRIMER_RIGHT_13_TM=55.702
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=994
PRIMER_PAIR_14_PENALTY=7.434772
PRIMER_LEFT_14_PENALTY=2.721451
PRIMER_RIGHT_14_PENALTY=4.713321
PRIMER_LEFT_14_SEQUENCE=CAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_14_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_14=755,22
PRIMER_RIGHT_14=1750,20
PRIMER_LEFT_14_TM=60.721
PRIMER_RIGHT_14_TM=55.287
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=6.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=996
PRIMER_PAIR_15_PENALTY=7.434772
PRIMER_LEFT_15_PENALTY=2.721451
PRIMER_RIGHT_15_PENALTY=4.713321
PRIMER_LEFT_15_SEQUENCE=ACAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_15_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_15=754,22
PRIMER_RIGHT_15=1750,20
PRIMER_LEFT_15_TM=60.721
PRIMER_RIGHT_15_TM=55.287
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=6.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=997
PRIMER_PAIR_16_PENALTY=7.497898
PRIMER_LEFT_16_PENALTY=3.243906
PRIMER_RIGHT_16_PENALTY=4.253992
PRIMER_LEFT_16_SEQUENCE=TCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_16_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_16=759,20
PRIMER_RIGHT_16=1753,22
PRIMER_LEFT_16_TM=63.244
PRIMER_RIGHT_16_TM=57.746
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=5.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=995
PRIMER_PAIR_17_PENALTY=7.541924
PRIMER_LEFT_17_PENALTY=3.243906
PRIMER_RIGHT_17_PENALTY=4.298018
PRIMER_LEFT_17_SEQUENCE=TCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_17_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_17=759,20
PRIMER_RIGHT_17=1749,20
PRIMER_LEFT_17_TM=63.244
PRIMER_RIGHT_17_TM=55.702
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=991
PRIMER_PAIR_18_PENALTY=7.558409
PRIMER_LEFT_18_PENALTY=2.845089
PRIMER_RIGHT_18_PENALTY=4.713321
PRIMER_LEFT_18_SEQUENCE=AAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_18_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_18=756,20
PRIMER_RIGHT_18=1750,20
PRIMER_LEFT_18_TM=57.155
PRIMER_RIGHT_18_TM=55.287
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=6.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=995
PRIMER_PAIR_19_PENALTY=7.654808
PRIMER_LEFT_19_PENALTY=2.941487
PRIMER_RIGHT_19_PENALTY=4.713321
PRIMER_LEFT_19_SEQUENCE=AAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_19_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_19=756,21
PRIMER_RIGHT_19=1750,20
PRIMER_LEFT_19_TM=58.059
PRIMER_RIGHT_19_TM=55.287
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=995
PRIMER_PAIR_20_PENALTY=7.659784
PRIMER_LEFT_20_PENALTY=3.405792
PRIMER_RIGHT_20_PENALTY=4.253992
PRIMER_LEFT_20_SEQUENCE=AATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_20_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_20=757,20
PRIMER_RIGHT_20=1753,22
PRIMER_LEFT_20_TM=56.594
PRIMER_RIGHT_20_TM=57.746
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=5.6000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=997
PRIMER_PAIR_21_PENALTY=7.703810
PRIMER_LEFT_21_PENALTY=3.405792
PRIMER_RIGHT_21_PENALTY=4.298018
PRIMER_LEFT_21_SEQUENCE=AATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_21_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_21=757,20
PRIMER_RIGHT_21=1749,20
PRIMER_LEFT_21_TM=56.594
PRIMER_RIGHT_21_TM=55.702
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=993
PRIMER_PAIR_22_PENALTY=7.879764
PRIMER_LEFT_22_PENALTY=1.054343
PRIMER_RIGHT_22_PENALTY=6.825421
PRIMER_LEFT_22_SEQUENCE=CAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_22_SEQUENCE=CTCTGTTGAAGGAGAAGAAAAG
PRIMER_LEFT_22=755,21
PRIMER_RIGHT_22=1708,22
PRIMER_LEFT_22_TM=59.946
PRIMER_RIGHT_22_TM=55.175
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=954
PRIMER_PAIR_23_PENALTY=7.889508
PRIMER_LEFT_23_PENALTY=2.721451
PRIMER_RIGHT_23_PENALTY=5.168056
PRIMER_LEFT_23_SEQUENCE=CAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_23_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_23=755,22
PRIMER_RIGHT_23=1753,21
PRIMER_LEFT_23_TM=60.721
PRIMER_RIGHT_23_TM=55.832
PRIMER_LEFT_23_GC_PERCENT=45.455
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=5.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=999
PRIMER_PAIR_24_PENALTY=7.889508
PRIMER_LEFT_24_PENALTY=2.721451
PRIMER_RIGHT_24_PENALTY=5.168056
PRIMER_LEFT_24_SEQUENCE=ACAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_24_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_24=754,22
PRIMER_RIGHT_24=1753,21
PRIMER_LEFT_24_TM=60.721
PRIMER_RIGHT_24_TM=55.832
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=5.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=1000
PRIMER_PAIR_25_PENALTY=7.957227
PRIMER_LEFT_25_PENALTY=3.243906
PRIMER_RIGHT_25_PENALTY=4.713321
PRIMER_LEFT_25_SEQUENCE=TCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_25_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_25=759,20
PRIMER_RIGHT_25=1750,20
PRIMER_LEFT_25_TM=63.244
PRIMER_RIGHT_25_TM=55.287
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.9000
PRIMER_RIGHT_25_END_STABILITY=6.0000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=992
PRIMER_PAIR_26_PENALTY=8.013145
PRIMER_LEFT_26_PENALTY=2.845089
PRIMER_RIGHT_26_PENALTY=5.168056
PRIMER_LEFT_26_SEQUENCE=AAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_26_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_26=756,20
PRIMER_RIGHT_26=1753,21
PRIMER_LEFT_26_TM=57.155
PRIMER_RIGHT_26_TM=55.832
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=5.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=998
PRIMER_PAIR_27_PENALTY=8.109543
PRIMER_LEFT_27_PENALTY=2.941487
PRIMER_RIGHT_27_PENALTY=5.168056
PRIMER_LEFT_27_SEQUENCE=AAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_27_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_27=756,21
PRIMER_RIGHT_27=1753,21
PRIMER_LEFT_27_TM=58.059
PRIMER_RIGHT_27_TM=55.832
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=5.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=998
PRIMER_PAIR_28_PENALTY=8.119113
PRIMER_LEFT_28_PENALTY=3.405792
PRIMER_RIGHT_28_PENALTY=4.713321
PRIMER_LEFT_28_SEQUENCE=AATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_28_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_28=757,20
PRIMER_RIGHT_28=1750,20
PRIMER_LEFT_28_TM=56.594
PRIMER_RIGHT_28_TM=55.287
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=994
PRIMER_PAIR_29_PENALTY=8.295410
PRIMER_LEFT_29_PENALTY=2.721451
PRIMER_RIGHT_29_PENALTY=5.573958
PRIMER_LEFT_29_SEQUENCE=CAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_29_SEQUENCE=GTAGCAAAGCTCCAACATAGAA
PRIMER_LEFT_29=755,22
PRIMER_RIGHT_29=1752,22
PRIMER_LEFT_29_TM=60.721
PRIMER_RIGHT_29_TM=56.426
PRIMER_LEFT_29_GC_PERCENT=45.455
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=6.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=998
PRIMER_PAIR_30_PENALTY=8.295410
PRIMER_LEFT_30_PENALTY=2.721451
PRIMER_RIGHT_30_PENALTY=5.573958
PRIMER_LEFT_30_SEQUENCE=ACAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_30_SEQUENCE=GTAGCAAAGCTCCAACATAGAA
PRIMER_LEFT_30=754,22
PRIMER_RIGHT_30=1752,22
PRIMER_LEFT_30_TM=60.721
PRIMER_RIGHT_30_TM=56.426
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=6.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=999
PRIMER_PAIR_31_PENALTY=8.411962
PRIMER_LEFT_31_PENALTY=3.243906
PRIMER_RIGHT_31_PENALTY=5.168056
PRIMER_LEFT_31_SEQUENCE=TCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_31_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_31=759,20
PRIMER_RIGHT_31=1753,21
PRIMER_LEFT_31_TM=63.244
PRIMER_RIGHT_31_TM=55.832
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.9000
PRIMER_RIGHT_31_END_STABILITY=5.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=995
PRIMER_PAIR_32_PENALTY=8.419047
PRIMER_LEFT_32_PENALTY=2.845089
PRIMER_RIGHT_32_PENALTY=5.573958
PRIMER_LEFT_32_SEQUENCE=AAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_32_SEQUENCE=GTAGCAAAGCTCCAACATAGAA
PRIMER_LEFT_32=756,20
PRIMER_RIGHT_32=1752,22
PRIMER_LEFT_32_TM=57.155
PRIMER_RIGHT_32_TM=56.426
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=6.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=997
PRIMER_PAIR_33_PENALTY=8.515445
PRIMER_LEFT_33_PENALTY=2.941487
PRIMER_RIGHT_33_PENALTY=5.573958
PRIMER_LEFT_33_SEQUENCE=AAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_33_SEQUENCE=GTAGCAAAGCTCCAACATAGAA
PRIMER_LEFT_33=756,21
PRIMER_RIGHT_33=1752,22
PRIMER_LEFT_33_TM=58.059
PRIMER_RIGHT_33_TM=56.426
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=40.909
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=6.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=997
PRIMER_PAIR_34_PENALTY=8.573848
PRIMER_LEFT_34_PENALTY=3.405792
PRIMER_RIGHT_34_PENALTY=5.168056
PRIMER_LEFT_34_SEQUENCE=AATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_34_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_34=757,20
PRIMER_RIGHT_34=1753,21
PRIMER_LEFT_34_TM=56.594
PRIMER_RIGHT_34_TM=55.832
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=5.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=997
PRIMER_PAIR_35_PENALTY=8.593074
PRIMER_LEFT_35_PENALTY=4.339082
PRIMER_RIGHT_35_PENALTY=4.253992
PRIMER_LEFT_35_SEQUENCE=ATCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_35_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_35=758,21
PRIMER_RIGHT_35=1753,22
PRIMER_LEFT_35_TM=63.339
PRIMER_RIGHT_35_TM=57.746
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.9000
PRIMER_RIGHT_35_END_STABILITY=5.6000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=996
PRIMER_PAIR_36_PENALTY=8.637100
PRIMER_LEFT_36_PENALTY=4.339082
PRIMER_RIGHT_36_PENALTY=4.298018
PRIMER_LEFT_36_SEQUENCE=ATCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_36_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_36=758,21
PRIMER_RIGHT_36=1749,20
PRIMER_LEFT_36_TM=63.339
PRIMER_RIGHT_36_TM=55.702
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=992
PRIMER_PAIR_37_PENALTY=8.686437
PRIMER_LEFT_37_PENALTY=1.861015
PRIMER_RIGHT_37_PENALTY=6.825421
PRIMER_LEFT_37_SEQUENCE=CTCTCGGCTAATGGGTTTGG
PRIMER_RIGHT_37_SEQUENCE=CTCTGTTGAAGGAGAAGAAAAG
PRIMER_LEFT_37=715,20
PRIMER_RIGHT_37=1708,22
PRIMER_LEFT_37_TM=61.861
PRIMER_RIGHT_37_TM=55.175
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.8000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=994
PRIMER_PAIR_38_PENALTY=8.696036
PRIMER_LEFT_38_PENALTY=4.442044
PRIMER_RIGHT_38_PENALTY=4.253992
PRIMER_LEFT_38_SEQUENCE=ACAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_38_SEQUENCE=TGTAGCAAAGCTCCAACATAGA
PRIMER_LEFT_38=754,23
PRIMER_RIGHT_38=1753,22
PRIMER_LEFT_38_TM=61.442
PRIMER_RIGHT_38_TM=57.746
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=5.6000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=1000
PRIMER_PAIR_39_PENALTY=8.740062
PRIMER_LEFT_39_PENALTY=4.442044
PRIMER_RIGHT_39_PENALTY=4.298018
PRIMER_LEFT_39_SEQUENCE=ACAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_39_SEQUENCE=GCAAAGCTCCAACATAGAAA
PRIMER_LEFT_39=754,23
PRIMER_RIGHT_39=1749,20
PRIMER_LEFT_39_TM=61.442
PRIMER_RIGHT_39_TM=55.702
PRIMER_LEFT_39_GC_PERCENT=43.478
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=996
PRIMER_PAIR_40_PENALTY=8.817864
PRIMER_LEFT_40_PENALTY=3.243906
PRIMER_RIGHT_40_PENALTY=5.573958
PRIMER_LEFT_40_SEQUENCE=TCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_40_SEQUENCE=GTAGCAAAGCTCCAACATAGAA
PRIMER_LEFT_40=759,20
PRIMER_RIGHT_40=1752,22
PRIMER_LEFT_40_TM=63.244
PRIMER_RIGHT_40_TM=56.426
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=6.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=994
PRIMER_PAIR_41_PENALTY=8.979750
PRIMER_LEFT_41_PENALTY=3.405792
PRIMER_RIGHT_41_PENALTY=5.573958
PRIMER_LEFT_41_SEQUENCE=AATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_41_SEQUENCE=GTAGCAAAGCTCCAACATAGAA
PRIMER_LEFT_41=757,20
PRIMER_RIGHT_41=1752,22
PRIMER_LEFT_41_TM=56.594
PRIMER_RIGHT_41_TM=56.426
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=6.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=996
PRIMER_PAIR_42_PENALTY=9.052403
PRIMER_LEFT_42_PENALTY=4.339082
PRIMER_RIGHT_42_PENALTY=4.713321
PRIMER_LEFT_42_SEQUENCE=ATCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_42_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_42=758,21
PRIMER_RIGHT_42=1750,20
PRIMER_LEFT_42_TM=63.339
PRIMER_RIGHT_42_TM=55.287
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=6.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=993
PRIMER_PAIR_43_PENALTY=9.155365
PRIMER_LEFT_43_PENALTY=4.442044
PRIMER_RIGHT_43_PENALTY=4.713321
PRIMER_LEFT_43_SEQUENCE=ACAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_43_SEQUENCE=AGCAAAGCTCCAACATAGAA
PRIMER_LEFT_43=754,23
PRIMER_RIGHT_43=1750,20
PRIMER_LEFT_43_TM=61.442
PRIMER_RIGHT_43_TM=55.287
PRIMER_LEFT_43_GC_PERCENT=43.478
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=6.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=997
PRIMER_PAIR_44_PENALTY=9.507138
PRIMER_LEFT_44_PENALTY=4.339082
PRIMER_RIGHT_44_PENALTY=5.168056
PRIMER_LEFT_44_SEQUENCE=ATCCAGGTAATTGAGGCACGG
PRIMER_RIGHT_44_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_44=758,21
PRIMER_RIGHT_44=1753,21
PRIMER_LEFT_44_TM=63.339
PRIMER_RIGHT_44_TM=55.832
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.9000
PRIMER_RIGHT_44_END_STABILITY=5.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=996
PRIMER_PAIR_45_PENALTY=9.546873
PRIMER_LEFT_45_PENALTY=2.721451
PRIMER_RIGHT_45_PENALTY=6.825421
PRIMER_LEFT_45_SEQUENCE=CAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_45_SEQUENCE=CTCTGTTGAAGGAGAAGAAAAG
PRIMER_LEFT_45=755,22
PRIMER_RIGHT_45=1708,22
PRIMER_LEFT_45_TM=60.721
PRIMER_RIGHT_45_TM=55.175
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=954
PRIMER_PAIR_46_PENALTY=9.546873
PRIMER_LEFT_46_PENALTY=2.721451
PRIMER_RIGHT_46_PENALTY=6.825421
PRIMER_LEFT_46_SEQUENCE=ACAAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_46_SEQUENCE=CTCTGTTGAAGGAGAAGAAAAG
PRIMER_LEFT_46=754,22
PRIMER_RIGHT_46=1708,22
PRIMER_LEFT_46_TM=60.721
PRIMER_RIGHT_46_TM=55.175
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=955
PRIMER_PAIR_47_PENALTY=9.610100
PRIMER_LEFT_47_PENALTY=4.442044
PRIMER_RIGHT_47_PENALTY=5.168056
PRIMER_LEFT_47_SEQUENCE=ACAAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_47_SEQUENCE=TGTAGCAAAGCTCCAACATAG
PRIMER_LEFT_47=754,23
PRIMER_RIGHT_47=1753,21
PRIMER_LEFT_47_TM=61.442
PRIMER_RIGHT_47_TM=55.832
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=5.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=1000
PRIMER_PAIR_48_PENALTY=9.670510
PRIMER_LEFT_48_PENALTY=2.845089
PRIMER_RIGHT_48_PENALTY=6.825421
PRIMER_LEFT_48_SEQUENCE=AAATCCAGGTAATTGAGGCA
PRIMER_RIGHT_48_SEQUENCE=CTCTGTTGAAGGAGAAGAAAAG
PRIMER_LEFT_48=756,20
PRIMER_RIGHT_48=1708,22
PRIMER_LEFT_48_TM=57.155
PRIMER_RIGHT_48_TM=55.175
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=953
PRIMER_PAIR_49_PENALTY=9.766908
PRIMER_LEFT_49_PENALTY=2.941487
PRIMER_RIGHT_49_PENALTY=6.825421
PRIMER_LEFT_49_SEQUENCE=AAATCCAGGTAATTGAGGCAC
PRIMER_RIGHT_49_SEQUENCE=CTCTGTTGAAGGAGAAGAAAAG
PRIMER_LEFT_49=756,21
PRIMER_RIGHT_49=1708,22
PRIMER_LEFT_49_TM=58.059
PRIMER_RIGHT_49_TM=55.175
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=953
=
SEQUENCE_ID=MAGIC.338_3_15555727_15555733_no-0_oy-0_918146_7760_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTTACCAANTACTTACGATTTTNCTACNAAATTTCCTNCAAAACANANGGTAGNAAAATGGNAGGAAATTTTCTACGAAAATCNNATAAAATNTANAGTCGTAGGAATATTTGTAGGAAATNGGAANNAATTTGATGGGAAAATANTTGGTANNAATTTGGTAGGAAACTTTCTTTCNAATTTTCTACGAAANNTTTANCANANNNNNNNNNNNANNAAATTGGTAGGACATACTCCTTCATATTTCCTANGAAAACAAAGTCGTAGGATNNNNNNNNNNNNTTTGTAGGAATAATATTAATAGAGTTATAACTATTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_5653128_5653547_po-0_bur-0_22681_40769_TRUE
SEQUENCE_TARGET=950,519
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACTANTANTTNAAACAAAATTTGANTTATTATAATTACTTACNTATATTTTTCCANTATTAACACATTGNATCCGAAATTTNTTTCCATAAAGTNAGGTTTCACAANNACTCACNAAAANAATCTGAGTTATTATAATTTTNCCCNATTATTATTNTNTTACACTTTGAGTCCAAAATTTATAANNTTCTTAATTATTGCAANAAANAAAAACTGAGTTATTAATTTACAAATATCCTTTTNACANTTNTTNATCAAAACTCANCNGGGANAAAAATATCTCTTTCTTCTTTTGTCNGATTTTAANTTTGGTGATACAATTTAATTTTTGCATTACTATAAAATCTAACCAACATGTAAAAATGTNATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNCACACTTNTGACTTATNAAATAGCTANAGGTTTNATTCNTCTTATNAANNAAANCTCNGGGATATTCTACACACCAAAAAACANATATTCGAGATCTTCTAGTGTTAGTTGAATCGNAGAACTTTTGCCANTNTTTTTCACCCAATGCAGATGCAACAATGTTCCTTTTACGTTCCTGAAATAAAANTAACAATAGAGTGGAGTGAGTCNNGTACACTATACAAAGAAAAAGATCTTGATAGCTTTGGCCTTTGGAACNAAAAANNCAATTAAATNCNTGAAGAGTCAAAATTCGTTCCTCAACGGTATTTTTGATGGCAATACGAGTNACCNTAACAGCNCGAGTTTGTCCGATACGATGAGCTCGATCAATAGCTTGATCCTCTGTTGTTGGATTCCACCATAGGTCCAGTAGAATGACATGACTTGCAGCTACCATATTCAATCNAAGGTTTCCAGCTNNAAGAGACATCAACATNGCANCTGTTTCATAATCATTACATTTGTCTTTGAGAAACGTAAAAATGAGATTCTTTGAATGCAAAACATATAATATNTCTGGATCATTGTTGAATTCTTTTACAGCTATGTCTCTTGCTACTAGAGACATTGTACCATCTAATCTTCTGAATTTTATGCGGTTTTGGNCAAAAGAATGCTCAACAAAGTCAAGCATACCAGTCCACTNATACTANTGTCTTTATTGGTNGANTGTCTGGACTGCCTTGCTCTGCAAGCGACTGAAGAATCTCTATCACAGCTCGGATTTTCGATGAAATAAACTCGCCTTGANGNAACTAAAGCATTTTTATCTTCAGGATCATCATAATCGTTGATGCAACTTCTAACTGCAGATTCAGTGAAAACAACATCATGTTTAAGCTGGCTGTGGCAATTAAGTGCAGGGCNCGTATTGNTATCCCCGTTAATGTTTACAGACACACACTCATAACAAAACACATGGCCACACAAAGTAACAACAGGGNCTTTTGGTGGATCCTGCAGAGAANATCATAAGAGTAAAAAAAAGATTATTAATTAGAACATAATGAGAGAGCTTGAAGAGACAAACAATGCTCAATCATTACAATGTACTTACNNTACAAACAGAGCAGGTGGTNGAGGANAATTTCAAGAGATCGAGGAACATGATTAGATTCTCTCTAGGNGCTACTCGAACTCCATCTGACATTTTTCTTGTAGTATCTGAGNGACNATATCCGTTAACAAGTTGTGGATGGTTACAAGCTTGGCGTAGTCGCAAAAGCATCACCAAAAGATAAGCCATGTGTTCATGCAAAGTCCCATCAGCAGCNTATTCCTATAGAATAAGAGTAGAAAATGTCATATAACAAGGTAGAAAGTCACCATTTATTCACATATATATGATAATNNTATTATTGAAGAATCACCTCAAACTTCCAACGTGAATTCAATTCAAGCTTCCTGTAGAAAGACCATTCNACTACTGNAAGGTCCTTTTTGCTCAAATTAACTTNCTTNAAGGTAGATTNATTATAGGTTGTCCATCAAGTAATGTTCCTAAATAGCAAAACATAAAAGAAAATTAGCACAAACTACAAAAGNANNNNNNTATATATATATATAGTAAGAGCAAACGAATACCTTTGGTGCGGCGCAACATTATACCCCTTAGAATAGCTTGAAGCTTCTTGTAACNATGAAGAGGCTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.982980
PRIMER_LEFT_0_PENALTY=2.448092
PRIMER_RIGHT_0_PENALTY=0.534888
PRIMER_LEFT_0_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_0_SEQUENCE=CTTTGTGTGGCCATGTGTTT
PRIMER_LEFT_0=864,20
PRIMER_RIGHT_0=1698,20
PRIMER_LEFT_0_TM=62.448
PRIMER_RIGHT_0_TM=59.465
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=835
PRIMER_PAIR_1_PENALTY=3.106168
PRIMER_LEFT_1_PENALTY=2.448092
PRIMER_RIGHT_1_PENALTY=0.658076
PRIMER_LEFT_1_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_1_SEQUENCE=CACTTAATTGCCACAGCCAG
PRIMER_LEFT_1=864,20
PRIMER_RIGHT_1=1626,20
PRIMER_LEFT_1_TM=62.448
PRIMER_RIGHT_1_TM=59.342
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=763
PRIMER_PAIR_2_PENALTY=3.192349
PRIMER_LEFT_2_PENALTY=2.448092
PRIMER_RIGHT_2_PENALTY=0.744257
PRIMER_LEFT_2_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_2_SEQUENCE=TCATCGAAAATCCGAGCTGT
PRIMER_LEFT_2=864,20
PRIMER_RIGHT_2=1500,20
PRIMER_LEFT_2_TM=62.448
PRIMER_RIGHT_2_TM=60.744
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=637
PRIMER_PAIR_3_PENALTY=3.462098
PRIMER_LEFT_3_PENALTY=3.326198
PRIMER_RIGHT_3_PENALTY=0.135900
PRIMER_LEFT_3_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_3_SEQUENCE=TTTGTGTGGCCATGTGTTTT
PRIMER_LEFT_3=881,22
PRIMER_RIGHT_3=1697,20
PRIMER_LEFT_3_TM=58.674
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=817
PRIMER_PAIR_4_PENALTY=3.539547
PRIMER_LEFT_4_PENALTY=2.448092
PRIMER_RIGHT_4_PENALTY=1.091455
PRIMER_LEFT_4_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_4_SEQUENCE=ACTTTGTGTGGCCATGTGTT
PRIMER_LEFT_4=864,20
PRIMER_RIGHT_4=1699,20
PRIMER_LEFT_4_TM=62.448
PRIMER_RIGHT_4_TM=58.909
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=836
PRIMER_PAIR_5_PENALTY=3.623997
PRIMER_LEFT_5_PENALTY=2.448092
PRIMER_RIGHT_5_PENALTY=1.175904
PRIMER_LEFT_5_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_5_SEQUENCE=TCTGCAGGATCCACCAAAAG
PRIMER_LEFT_5=864,20
PRIMER_RIGHT_5=1730,20
PRIMER_LEFT_5_TM=62.448
PRIMER_RIGHT_5_TM=61.176
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=867
PRIMER_PAIR_6_PENALTY=3.775695
PRIMER_LEFT_6_PENALTY=2.448092
PRIMER_RIGHT_6_PENALTY=1.327603
PRIMER_LEFT_6_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_6_SEQUENCE=ACTTTGTGTGGCCATGTGTTT
PRIMER_LEFT_6=864,20
PRIMER_RIGHT_6=1699,21
PRIMER_LEFT_6_TM=62.448
PRIMER_RIGHT_6_TM=60.328
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=836
PRIMER_PAIR_7_PENALTY=3.861086
PRIMER_LEFT_7_PENALTY=3.326198
PRIMER_RIGHT_7_PENALTY=0.534888
PRIMER_LEFT_7_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_7_SEQUENCE=CTTTGTGTGGCCATGTGTTT
PRIMER_LEFT_7=881,22
PRIMER_RIGHT_7=1698,20
PRIMER_LEFT_7_TM=58.674
PRIMER_RIGHT_7_TM=59.465
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=818
PRIMER_PAIR_8_PENALTY=3.984274
PRIMER_LEFT_8_PENALTY=3.326198
PRIMER_RIGHT_8_PENALTY=0.658076
PRIMER_LEFT_8_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_8_SEQUENCE=CACTTAATTGCCACAGCCAG
PRIMER_LEFT_8=881,22
PRIMER_RIGHT_8=1626,20
PRIMER_LEFT_8_TM=58.674
PRIMER_RIGHT_8_TM=59.342
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=746
PRIMER_PAIR_9_PENALTY=4.007819
PRIMER_LEFT_9_PENALTY=3.326198
PRIMER_RIGHT_9_PENALTY=0.681621
PRIMER_LEFT_9_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_9_SEQUENCE=GTGTGGCCATGTGTTTTGTT
PRIMER_LEFT_9=881,22
PRIMER_RIGHT_9=1694,20
PRIMER_LEFT_9_TM=58.674
PRIMER_RIGHT_9_TM=59.318
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=814
PRIMER_PAIR_10_PENALTY=4.070455
PRIMER_LEFT_10_PENALTY=3.326198
PRIMER_RIGHT_10_PENALTY=0.744257
PRIMER_LEFT_10_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_10_SEQUENCE=TCATCGAAAATCCGAGCTGT
PRIMER_LEFT_10=881,22
PRIMER_RIGHT_10=1500,20
PRIMER_LEFT_10_TM=58.674
PRIMER_RIGHT_10_TM=60.744
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=620
PRIMER_PAIR_11_PENALTY=4.221635
PRIMER_LEFT_11_PENALTY=3.326198
PRIMER_RIGHT_11_PENALTY=0.895437
PRIMER_LEFT_11_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_11_SEQUENCE=TGTGTGGCCATGTGTTTTGT
PRIMER_LEFT_11=881,22
PRIMER_RIGHT_11=1695,20
PRIMER_LEFT_11_TM=58.674
PRIMER_RIGHT_11_TM=60.895
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=815
PRIMER_PAIR_12_PENALTY=4.417653
PRIMER_LEFT_12_PENALTY=3.326198
PRIMER_RIGHT_12_PENALTY=1.091455
PRIMER_LEFT_12_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_12_SEQUENCE=ACTTTGTGTGGCCATGTGTT
PRIMER_LEFT_12=881,22
PRIMER_RIGHT_12=1699,20
PRIMER_LEFT_12_TM=58.674
PRIMER_RIGHT_12_TM=58.909
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=819
PRIMER_PAIR_13_PENALTY=4.484830
PRIMER_LEFT_13_PENALTY=2.448092
PRIMER_RIGHT_13_PENALTY=2.036738
PRIMER_LEFT_13_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_13_SEQUENCE=TACTTTGTGTGGCCATGTGTT
PRIMER_LEFT_13=864,20
PRIMER_RIGHT_13=1700,21
PRIMER_LEFT_13_TM=62.448
PRIMER_RIGHT_13_TM=58.963
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=837
PRIMER_PAIR_14_PENALTY=4.484830
PRIMER_LEFT_14_PENALTY=2.448092
PRIMER_RIGHT_14_PENALTY=2.036738
PRIMER_LEFT_14_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_14_SEQUENCE=TTACTTTGTGTGGCCATGTGT
PRIMER_LEFT_14=864,20
PRIMER_RIGHT_14=1701,21
PRIMER_LEFT_14_TM=62.448
PRIMER_RIGHT_14_TM=58.963
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=838
PRIMER_PAIR_15_PENALTY=4.557550
PRIMER_LEFT_15_PENALTY=2.448092
PRIMER_RIGHT_15_PENALTY=2.109458
PRIMER_LEFT_15_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_15_SEQUENCE=TGGCCATGTGTTTTGTTATG
PRIMER_LEFT_15=864,20
PRIMER_RIGHT_15=1691,20
PRIMER_LEFT_15_TM=62.448
PRIMER_RIGHT_15_TM=57.891
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=828
PRIMER_PAIR_16_PENALTY=4.622601
PRIMER_LEFT_16_PENALTY=2.448092
PRIMER_RIGHT_16_PENALTY=2.174509
PRIMER_LEFT_16_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_16_SEQUENCE=GTTACTTTGTGTGGCCATGTGT
PRIMER_LEFT_16=864,20
PRIMER_RIGHT_16=1702,22
PRIMER_LEFT_16_TM=62.448
PRIMER_RIGHT_16_TM=59.825
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=839
PRIMER_PAIR_17_PENALTY=4.637681
PRIMER_LEFT_17_PENALTY=2.448092
PRIMER_RIGHT_17_PENALTY=2.189589
PRIMER_LEFT_17_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_17_SEQUENCE=GTGGCCATGTGTTTTGTTATG
PRIMER_LEFT_17=864,20
PRIMER_RIGHT_17=1692,21
PRIMER_LEFT_17_TM=62.448
PRIMER_RIGHT_17_TM=58.810
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=829
PRIMER_PAIR_18_PENALTY=4.653801
PRIMER_LEFT_18_PENALTY=3.326198
PRIMER_RIGHT_18_PENALTY=1.327603
PRIMER_LEFT_18_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_18_SEQUENCE=ACTTTGTGTGGCCATGTGTTT
PRIMER_LEFT_18=881,22
PRIMER_RIGHT_18=1699,21
PRIMER_LEFT_18_TM=58.674
PRIMER_RIGHT_18_TM=60.328
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=819
PRIMER_PAIR_19_PENALTY=4.756258
PRIMER_LEFT_19_PENALTY=3.326198
PRIMER_RIGHT_19_PENALTY=1.430060
PRIMER_LEFT_19_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_19_SEQUENCE=TTGTGTGGCCATGTGTTTTG
PRIMER_LEFT_19=881,22
PRIMER_RIGHT_19=1696,20
PRIMER_LEFT_19_TM=58.674
PRIMER_RIGHT_19_TM=61.430
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=816
PRIMER_PAIR_20_PENALTY=4.773994
PRIMER_LEFT_20_PENALTY=2.448092
PRIMER_RIGHT_20_PENALTY=2.325902
PRIMER_LEFT_20_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_20_SEQUENCE=TACTTTGTGTGGCCATGTGTTT
PRIMER_LEFT_20=864,20
PRIMER_RIGHT_20=1700,22
PRIMER_LEFT_20_TM=62.448
PRIMER_RIGHT_20_TM=60.326
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=837
PRIMER_PAIR_21_PENALTY=4.773994
PRIMER_LEFT_21_PENALTY=2.448092
PRIMER_RIGHT_21_PENALTY=2.325902
PRIMER_LEFT_21_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_21_SEQUENCE=TTACTTTGTGTGGCCATGTGTT
PRIMER_LEFT_21=864,20
PRIMER_RIGHT_21=1701,22
PRIMER_LEFT_21_TM=62.448
PRIMER_RIGHT_21_TM=60.326
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=838
PRIMER_PAIR_22_PENALTY=5.010841
PRIMER_LEFT_22_PENALTY=2.448092
PRIMER_RIGHT_22_PENALTY=2.562749
PRIMER_LEFT_22_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_22_SEQUENCE=TACTTTGTGTGGCCATGTGT
PRIMER_LEFT_22=864,20
PRIMER_RIGHT_22=1700,20
PRIMER_LEFT_22_TM=62.448
PRIMER_RIGHT_22_TM=57.437
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=837
PRIMER_PAIR_23_PENALTY=5.158183
PRIMER_LEFT_23_PENALTY=3.326198
PRIMER_RIGHT_23_PENALTY=1.831985
PRIMER_LEFT_23_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_23_SEQUENCE=CTTTGTGTGGCCATGTGTTTT
PRIMER_LEFT_23=881,22
PRIMER_RIGHT_23=1698,21
PRIMER_LEFT_23_TM=58.674
PRIMER_RIGHT_23_TM=60.832
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=818
PRIMER_PAIR_24_PENALTY=5.230144
PRIMER_LEFT_24_PENALTY=2.448092
PRIMER_RIGHT_24_PENALTY=2.782051
PRIMER_LEFT_24_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_24_SEQUENCE=TGGCCATGTGTTTTGTTATGAG
PRIMER_LEFT_24=864,20
PRIMER_RIGHT_24=1691,22
PRIMER_LEFT_24_TM=62.448
PRIMER_RIGHT_24_TM=60.782
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=828
PRIMER_PAIR_25_PENALTY=5.362936
PRIMER_LEFT_25_PENALTY=3.326198
PRIMER_RIGHT_25_PENALTY=2.036738
PRIMER_LEFT_25_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_25_SEQUENCE=TACTTTGTGTGGCCATGTGTT
PRIMER_LEFT_25=881,22
PRIMER_RIGHT_25=1700,21
PRIMER_LEFT_25_TM=58.674
PRIMER_RIGHT_25_TM=58.963
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=820
PRIMER_PAIR_26_PENALTY=5.362936
PRIMER_LEFT_26_PENALTY=3.326198
PRIMER_RIGHT_26_PENALTY=2.036738
PRIMER_LEFT_26_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_26_SEQUENCE=TTACTTTGTGTGGCCATGTGT
PRIMER_LEFT_26=881,22
PRIMER_RIGHT_26=1701,21
PRIMER_LEFT_26_TM=58.674
PRIMER_RIGHT_26_TM=58.963
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=821
PRIMER_PAIR_27_PENALTY=5.435352
PRIMER_LEFT_27_PENALTY=2.448092
PRIMER_RIGHT_27_PENALTY=2.987259
PRIMER_LEFT_27_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_27_SEQUENCE=GGCCATGTGTTTTGTTATGAG
PRIMER_LEFT_27=864,20
PRIMER_RIGHT_27=1690,21
PRIMER_LEFT_27_TM=62.448
PRIMER_RIGHT_27_TM=58.013
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=827
PRIMER_PAIR_28_PENALTY=5.435656
PRIMER_LEFT_28_PENALTY=3.326198
PRIMER_RIGHT_28_PENALTY=2.109458
PRIMER_LEFT_28_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_28_SEQUENCE=TGGCCATGTGTTTTGTTATG
PRIMER_LEFT_28=881,22
PRIMER_RIGHT_28=1691,20
PRIMER_LEFT_28_TM=58.674
PRIMER_RIGHT_28_TM=57.891
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=811
PRIMER_PAIR_29_PENALTY=5.449839
PRIMER_LEFT_29_PENALTY=2.448092
PRIMER_RIGHT_29_PENALTY=3.001746
PRIMER_LEFT_29_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_29_SEQUENCE=TTCATCGAAAATCCGAGCTGT
PRIMER_LEFT_29=864,20
PRIMER_RIGHT_29=1501,21
PRIMER_LEFT_29_TM=62.448
PRIMER_RIGHT_29_TM=62.002
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=638
PRIMER_PAIR_30_PENALTY=5.500707
PRIMER_LEFT_30_PENALTY=3.326198
PRIMER_RIGHT_30_PENALTY=2.174509
PRIMER_LEFT_30_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_30_SEQUENCE=GTTACTTTGTGTGGCCATGTGT
PRIMER_LEFT_30=881,22
PRIMER_RIGHT_30=1702,22
PRIMER_LEFT_30_TM=58.674
PRIMER_RIGHT_30_TM=59.825
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=822
PRIMER_PAIR_31_PENALTY=5.515787
PRIMER_LEFT_31_PENALTY=3.326198
PRIMER_RIGHT_31_PENALTY=2.189589
PRIMER_LEFT_31_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_31_SEQUENCE=GTGGCCATGTGTTTTGTTATG
PRIMER_LEFT_31=881,22
PRIMER_RIGHT_31=1692,21
PRIMER_LEFT_31_TM=58.674
PRIMER_RIGHT_31_TM=58.810
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.2000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=812
PRIMER_PAIR_32_PENALTY=5.563334
PRIMER_LEFT_32_PENALTY=2.448092
PRIMER_RIGHT_32_PENALTY=3.115242
PRIMER_LEFT_32_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_32_SEQUENCE=GGCCATGTGTTTTGTTATGAGT
PRIMER_LEFT_32=864,20
PRIMER_RIGHT_32=1690,22
PRIMER_LEFT_32_TM=62.448
PRIMER_RIGHT_32_TM=58.885
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=827
PRIMER_PAIR_33_PENALTY=5.578130
PRIMER_LEFT_33_PENALTY=2.448092
PRIMER_RIGHT_33_PENALTY=3.130037
PRIMER_LEFT_33_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_33_SEQUENCE=GAAAATCCGAGCTGTGATAGAGA
PRIMER_LEFT_33=864,20
PRIMER_RIGHT_33=1495,23
PRIMER_LEFT_33_TM=62.448
PRIMER_RIGHT_33_TM=59.870
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=43.478
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=632
PRIMER_PAIR_34_PENALTY=5.652100
PRIMER_LEFT_34_PENALTY=3.326198
PRIMER_RIGHT_34_PENALTY=2.325902
PRIMER_LEFT_34_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_34_SEQUENCE=TACTTTGTGTGGCCATGTGTTT
PRIMER_LEFT_34=881,22
PRIMER_RIGHT_34=1700,22
PRIMER_LEFT_34_TM=58.674
PRIMER_RIGHT_34_TM=60.326
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=820
PRIMER_PAIR_35_PENALTY=5.652100
PRIMER_LEFT_35_PENALTY=3.326198
PRIMER_RIGHT_35_PENALTY=2.325902
PRIMER_LEFT_35_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_35_SEQUENCE=TTACTTTGTGTGGCCATGTGTT
PRIMER_LEFT_35=881,22
PRIMER_RIGHT_35=1701,22
PRIMER_LEFT_35_TM=58.674
PRIMER_RIGHT_35_TM=60.326
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=821
PRIMER_PAIR_36_PENALTY=5.887491
PRIMER_LEFT_36_PENALTY=2.448092
PRIMER_RIGHT_36_PENALTY=3.439399
PRIMER_LEFT_36_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_36_SEQUENCE=TGTCTGTAAACATTAACGGGG
PRIMER_LEFT_36=864,20
PRIMER_RIGHT_36=1665,21
PRIMER_LEFT_36_TM=62.448
PRIMER_RIGHT_36_TM=57.561
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=12.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=802
PRIMER_PAIR_37_PENALTY=5.888947
PRIMER_LEFT_37_PENALTY=3.326198
PRIMER_RIGHT_37_PENALTY=2.562749
PRIMER_LEFT_37_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_37_SEQUENCE=TACTTTGTGTGGCCATGTGT
PRIMER_LEFT_37=881,22
PRIMER_RIGHT_37=1700,20
PRIMER_LEFT_37_TM=58.674
PRIMER_RIGHT_37_TM=57.437
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=820
PRIMER_PAIR_38_PENALTY=6.021495
PRIMER_LEFT_38_PENALTY=2.448092
PRIMER_RIGHT_38_PENALTY=3.573403
PRIMER_LEFT_38_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_38_SEQUENCE=GTGTCTGTAAACATTAACGGGG
PRIMER_LEFT_38=864,20
PRIMER_RIGHT_38=1666,22
PRIMER_LEFT_38_TM=62.448
PRIMER_RIGHT_38_TM=58.427
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=12.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=803
PRIMER_PAIR_39_PENALTY=6.030194
PRIMER_LEFT_39_PENALTY=2.448092
PRIMER_RIGHT_39_PENALTY=3.582102
PRIMER_LEFT_39_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_39_SEQUENCE=TGTGGCCATGTGTTTTGTTATG
PRIMER_LEFT_39=864,20
PRIMER_RIGHT_39=1693,22
PRIMER_LEFT_39_TM=62.448
PRIMER_RIGHT_39_TM=61.582
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.2000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=830
PRIMER_PAIR_40_PENALTY=6.047184
PRIMER_LEFT_40_PENALTY=2.448092
PRIMER_RIGHT_40_PENALTY=3.599092
PRIMER_LEFT_40_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_40_SEQUENCE=CGAAAATCCGAGCTGTGATAGA
PRIMER_LEFT_40=864,20
PRIMER_RIGHT_40=1496,22
PRIMER_LEFT_40_TM=62.448
PRIMER_RIGHT_40_TM=61.599
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=5.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=633
PRIMER_PAIR_41_PENALTY=6.108250
PRIMER_LEFT_41_PENALTY=3.326198
PRIMER_RIGHT_41_PENALTY=2.782051
PRIMER_LEFT_41_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_41_SEQUENCE=TGGCCATGTGTTTTGTTATGAG
PRIMER_LEFT_41=881,22
PRIMER_RIGHT_41=1691,22
PRIMER_LEFT_41_TM=58.674
PRIMER_RIGHT_41_TM=60.782
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=811
PRIMER_PAIR_42_PENALTY=6.275149
PRIMER_LEFT_42_PENALTY=3.326198
PRIMER_RIGHT_42_PENALTY=2.948951
PRIMER_LEFT_42_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_42_SEQUENCE=CCCTGTTGTTACTTTGTGTGG
PRIMER_LEFT_42=881,22
PRIMER_RIGHT_42=1709,21
PRIMER_LEFT_42_TM=58.674
PRIMER_RIGHT_42_TM=58.051
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=829
PRIMER_PAIR_43_PENALTY=6.313457
PRIMER_LEFT_43_PENALTY=3.326198
PRIMER_RIGHT_43_PENALTY=2.987259
PRIMER_LEFT_43_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_43_SEQUENCE=GGCCATGTGTTTTGTTATGAG
PRIMER_LEFT_43=881,22
PRIMER_RIGHT_43=1690,21
PRIMER_LEFT_43_TM=58.674
PRIMER_RIGHT_43_TM=58.013
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=810
PRIMER_PAIR_44_PENALTY=6.327945
PRIMER_LEFT_44_PENALTY=3.326198
PRIMER_RIGHT_44_PENALTY=3.001746
PRIMER_LEFT_44_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_44_SEQUENCE=TTCATCGAAAATCCGAGCTGT
PRIMER_LEFT_44=881,22
PRIMER_RIGHT_44=1501,21
PRIMER_LEFT_44_TM=58.674
PRIMER_RIGHT_44_TM=62.002
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=621
PRIMER_PAIR_45_PENALTY=6.350356
PRIMER_LEFT_45_PENALTY=2.448092
PRIMER_RIGHT_45_PENALTY=3.902264
PRIMER_LEFT_45_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_45_SEQUENCE=AAAATCCGAGCTGTGATAGAGA
PRIMER_LEFT_45=864,20
PRIMER_RIGHT_45=1494,22
PRIMER_LEFT_45_TM=62.448
PRIMER_RIGHT_45_TM=58.098
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=631
PRIMER_PAIR_46_PENALTY=6.367310
PRIMER_LEFT_46_PENALTY=2.448092
PRIMER_RIGHT_46_PENALTY=3.919218
PRIMER_LEFT_46_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_46_SEQUENCE=GCCATGTGTTTTGTTATGAGTG
PRIMER_LEFT_46=864,20
PRIMER_RIGHT_46=1689,22
PRIMER_LEFT_46_TM=62.448
PRIMER_RIGHT_46_TM=58.081
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=826
PRIMER_PAIR_47_PENALTY=6.403054
PRIMER_LEFT_47_PENALTY=2.448092
PRIMER_RIGHT_47_PENALTY=3.954962
PRIMER_LEFT_47_SEQUENCE=ACCCAATGCAGATGCAACAA
PRIMER_RIGHT_47_SEQUENCE=GAAAATCCGAGCTGTGATAGA
PRIMER_LEFT_47=864,20
PRIMER_RIGHT_47=1495,21
PRIMER_LEFT_47_TM=62.448
PRIMER_RIGHT_47_TM=57.045
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=5.6000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=632
PRIMER_PAIR_48_PENALTY=6.441440
PRIMER_LEFT_48_PENALTY=3.326198
PRIMER_RIGHT_48_PENALTY=3.115242
PRIMER_LEFT_48_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_48_SEQUENCE=GGCCATGTGTTTTGTTATGAGT
PRIMER_LEFT_48=881,22
PRIMER_RIGHT_48=1690,22
PRIMER_LEFT_48_TM=58.674
PRIMER_RIGHT_48_TM=58.885
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=810
PRIMER_PAIR_49_PENALTY=6.456235
PRIMER_LEFT_49_PENALTY=3.326198
PRIMER_RIGHT_49_PENALTY=3.130037
PRIMER_LEFT_49_SEQUENCE=CAATGTTCCTTTTACGTTCCTG
PRIMER_RIGHT_49_SEQUENCE=GAAAATCCGAGCTGTGATAGAGA
PRIMER_LEFT_49=881,22
PRIMER_RIGHT_49=1495,23
PRIMER_LEFT_49_TM=58.674
PRIMER_RIGHT_49_TM=59.870
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=43.478
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=615
=
SEQUENCE_ID=MAGIC.338_5_9622601_9623355_rsch-4_po-0_60578_441999_TRUE
SEQUENCE_TARGET=950,854
SEQUENCE_TEMPLATE=TNTTGGTAACGAGAATCTTTACAGTTCGATCATCTTCTATTTCACGAAAGCNGGGAAGTTGATACGTGCTGTGAATANATTTAGACATATGGTGAATAGTAGGAACTTGGAATGTAGACCAACGATGAGAACGTATCATATTCTCTTCAAAGCATTGTTGGGTAGAGGTAACAACTCTTTCANAANCCATNTGTATATGGAGACAGTAAGANCTTTGTTTCGANAAATGGTGGATAGTGGTNTTGAACCAGANGTATTTGCTTTGAACTGTTTGGTTAAAGGTTACGTGCTTTCGCTTCACGAGTCATCAGATGAGTGTGGTTTACNGTTGTGAACCGAATTNGTTTACTNATGATTATCTAATACATGGATTATGTGCTCAAGGTANGACCATNANTNCTAGAGAATTGCTTAGTGAGATGAAAGGNNAAGGCTTTGTTCCTAATGGGAAATCTTATAACTCTCTGGTTAATGCTTTTGCGCTTAGTGGTNAGATTGATGATGCGGTGAAATGTTTGTGGGAGATGATTGAGAATGGTCGTGTGGTTGATTTTATTANCTATAGNACACTTGTTGATGAGAGTTNTAGGAAAGGGAAGTANGATGAAGCGACGAGATTGTTGGNGATGTTGCGAGAGAAACAGCTTGTGGATATAGATTCTNATGATAAGCTTGTGAATGTTCTTCACAAGGATTTATANAAGNTGTACAAAATTGTGNTTCTTGGTTTGTTGTAATNTAGAAATTTTGGTGCAATTTATTTTATTGTTTTTGGTTTATGGTAGTGAACAAATAGTGAGTTAATGTAAAGTTTTGTAGAAGATGTACCAAATGGTGATTCTTGGTTTGTTGTNATNTCGAAATTTTGGTGCAATTTATTTTATTGTTTTTGTTATATGGTATTGGAGAAATAGTGAGTTTATGTAANTTTNCAGTTCTTCTTTTCTCCTNAATGNTGCCTTCANTTTGCGATGAATCAAGGTCTGNTTTTNACTTTGTTNTTNTTGCTTTNTGGGATCNGTTTATTGAAATTTTGAACCNTTTTGTTGGTTTTTCAGATACATGATAGTACGTAATGTGCCAGCTTTGGGTTGTGGTGATGATCTCATGAGATTATTCATGACTTATGGAGNAGTTGAAGAGTGAGTCTCTTTTTTGTTTCCTGCATTGTTCTTATGGACTATGATATTGTTTATTTGTTTGTTTGGATTTGATTCGTGTAGATGTAAACCTATGGATGNAGAAGACTGTGCGGAGTTCACGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCTAGGTTTTGATTCTTCTTTCTTTGATGATCAACTTGGCNTCACTGAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAAGAGGAAGTTGGATGAATCAAGTTTTTTGGGAAATCGGCTCCAAATCTCATATGCTCCTGAATACGAGAGCGTCAGTGACACAAAGGAGAAGTTANAAACTAGNAGGAAAGAAGTGCTTGCAAGACTGAACCGTAAGNTTTCTATTTACACTTCANCTNNTTTTACTGTCAGTTATTCTTCTTGCTACAACAAGAAAGATTATCATTGGGGAAAGTGGACAGAAACTGTGTTTCCTCAGTAGACTTAAAGCTGTCAATTAGTAATTGGGATTTATCATTGTACTCAAAGTGGATCATACCCTTGAACTGATTGCCATGTATGTTGCAGAAGCTAACATTTTGTTTATAGCATTGTATTAGTAAAGTTTTTGAGGTTAGAAAAGNNNNNNNNNNNNNNNNNNNNNNCAGTATAACTTCACA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_5666104_5666174_mt-0_hi-0_266_240956_TRUE
SEQUENCE_TARGET=950,170
SEQUENCE_TEMPLATE=TTTCTTTANAAGATTTCANTCTGNAACATGCATAGAAACTTGTCTCATNACCCTCGTAGTCNTATAGCAAGCATTAGTTTTATGATAGAGATGTTTCNGTTACGAAAGAAANTTTAAACCAACTTAAGTGGTTGTAAATCACGTTGTANTTTTTATGTTTATGTACAANTNCAAATTACAAATCTTCNTTTTTAATTATAATCTTTGCCACTGAAATTCACGATAATATCACTAAGGTTCGATGCAATTCGTCAAACATNTCACGTACTAAAATTTGTTNGGCGANNGANGGAGACATAGATGTAAATGGAAACGTNNACTCGTAAATTGTGTCATCTTTCGTGGCCCATNAANTNGAGATGGAGTTTTATCGTTTTCTATATCNNNNNNNNNNNNNNNNNNNNNNAANAAGGTTTTATTATAAAAAGTACCAAAGAAATGGACCGTAGTCACCATNNNNNNNNTTACATGGTTACATGGCGCGACACGTAGTGTCACTTAATGCTGCAAGTGTTTTTTCCTCTTTGTTTTTTGCCTCCCTGAATATTTTAAATTTGNTATATAAAATNTTTCTGCTGACGAGTTTTAGTTACTCTAGTTTCTAAAATGGTCTAGAAAAAGTTGTGNTATATAANTATAATAACTCGGTAANGGCTACGTACAGAGTCAGCCACGTAACANTAAATGGATGTGTCATGGACCCGTATTACACTCNNCATTATACACGNNTNNNGNNTANATNNNNTANNNNNNGATNANNNANNTTTNNNNTNTCAANNNNGANANANNTANTNNNGTANNNNNTNTTCNAAANNCATAGTNANTNTANNTNATNANAAATANGTNTTNANTNGTTTNNAAACNANTNNNCANNNNNNNANTTATTNTTNTNNCNNNNANTNANTNTGANTNNTTTNNNANNNGNAANTANCTATNTNNNNNNTGNNNNNNNAANNNNNNTTNTTTNNTNAANANTANNTNTNATTNTNNANNNTACANNNANNCCACAACTAGAAAGACGANTCCAAAAACNAAAGNAAATAGGAANTACTCGACCTTTNTTTTCTAACNTCAATCCACCACATNATATTTGTNGTTTTTAGCGNAATAATTNAGTTCTAACACACCACATGATTTGCTAAGCTACCAGTACTACTTTCTTTGTTAGGCCNTGAGAAATTGAATATTCCTTCCTCTTATTCTGCATATTCTTTGTATTTGTTTTGTTACCTACCTCTTCTAGTGTGTAGCCTACGTAAATATACACTCAAGTTGTTTTGATGTAACCNGTTTTCTCCACCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACACTACATATCAATTCNTACTTATTATAANTACACTNATTANATNTNAATGCATGAANNNAAACTCGAATTCGATATNNTNCGATTCGTCAAAAAANNNNNNNNNNNNNNNNNNTGGTATCAATTAGAGGTCTGAATTTGTTCAATATCGTCCACAATTCCCTACCTAAAAAATTAGAGCTTTTTGGACCAAATCCAAAAATGGATCTAAAAAGTGAAAACCAAAATATTCCTTTAAGAATCTACACAGATTCGAACCAAACCGGATTAAACCGNATAGTCNACGGCACGTGTGGAAACGCGNNNCGTTTCAAAAACATTTGGGGTANTNNNNNNNNNNNNNNNNNNNNNNNNATCAGGGTTTTAGGAGAAACCCAGGANGAAGAAGAAGATCGANATGGACTTAGAACACCAAACCATGGAGACGTTCCTGCGATGGGCAGCAGAGATTGGCATTTCAGATTCNATTGATTCTTCTCGATTTCGCGATTCGTGTCTTGGNCATTCTCTTTCCGTCNCCGACTTCNCTNACGCCGGCGGGTGCGTGGAATTGAATTCGAACTCTTTCATTTCTAAATCCNTNAAATNTGTCTTCTCATTNCTCGATTGATGNAATCAGGAGAGGTTTGGGAGCTGNTCGTGAGCTCAAGAAAGGAGAATTGNTTTTGAAAGTCCCCAGAAAAGCCTTGATGACTACAGAATCCATAATTGCTAAAG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.915209
PRIMER_LEFT_0_PENALTY=1.453747
PRIMER_RIGHT_0_PENALTY=1.461462
PRIMER_LEFT_0_SEQUENCE=GATGTGTCATGGACCCGTATT
PRIMER_RIGHT_0_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_0=687,21
PRIMER_RIGHT_0=1617,20
PRIMER_LEFT_0_TM=59.546
PRIMER_RIGHT_0_TM=61.461
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=931
PRIMER_PAIR_1_PENALTY=3.176275
PRIMER_LEFT_1_PENALTY=1.453747
PRIMER_RIGHT_1_PENALTY=1.722528
PRIMER_LEFT_1_SEQUENCE=GATGTGTCATGGACCCGTATT
PRIMER_RIGHT_1_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_1=687,21
PRIMER_RIGHT_1=1626,20
PRIMER_LEFT_1_TM=59.546
PRIMER_RIGHT_1_TM=58.277
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=940
PRIMER_PAIR_2_PENALTY=3.190614
PRIMER_LEFT_2_PENALTY=1.468086
PRIMER_RIGHT_2_PENALTY=1.722528
PRIMER_LEFT_2_SEQUENCE=GGCTACGTACAGAGTCAGCCA
PRIMER_RIGHT_2_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_2=652,21
PRIMER_RIGHT_2=1626,20
PRIMER_LEFT_2_TM=60.468
PRIMER_RIGHT_2_TM=58.277
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=975
PRIMER_PAIR_3_PENALTY=3.450263
PRIMER_LEFT_3_PENALTY=1.988801
PRIMER_RIGHT_3_PENALTY=1.461462
PRIMER_LEFT_3_SEQUENCE=CGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_3_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_3=657,21
PRIMER_RIGHT_3=1617,20
PRIMER_LEFT_3_TM=59.011
PRIMER_RIGHT_3_TM=61.461
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=961
PRIMER_PAIR_4_PENALTY=3.597442
PRIMER_LEFT_4_PENALTY=2.135980
PRIMER_RIGHT_4_PENALTY=1.461462
PRIMER_LEFT_4_SEQUENCE=ACGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_4_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_4=656,22
PRIMER_RIGHT_4=1617,20
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=61.461
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=962
PRIMER_PAIR_5_PENALTY=3.640278
PRIMER_LEFT_5_PENALTY=1.453747
PRIMER_RIGHT_5_PENALTY=2.186531
PRIMER_LEFT_5_SEQUENCE=GATGTGTCATGGACCCGTATT
PRIMER_RIGHT_5_SEQUENCE=CCGGTTTGGTTCGAATCTGT
PRIMER_LEFT_5=687,21
PRIMER_RIGHT_5=1618,20
PRIMER_LEFT_5_TM=59.546
PRIMER_RIGHT_5_TM=62.187
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.6000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=932
PRIMER_PAIR_6_PENALTY=3.711328
PRIMER_LEFT_6_PENALTY=1.988801
PRIMER_RIGHT_6_PENALTY=1.722528
PRIMER_LEFT_6_SEQUENCE=CGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_6_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_6=657,21
PRIMER_RIGHT_6=1626,20
PRIMER_LEFT_6_TM=59.011
PRIMER_RIGHT_6_TM=58.277
PRIMER_LEFT_6_GC_PERCENT=52.381
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.7000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=970
PRIMER_PAIR_7_PENALTY=3.858508
PRIMER_LEFT_7_PENALTY=2.135980
PRIMER_RIGHT_7_PENALTY=1.722528
PRIMER_LEFT_7_SEQUENCE=ACGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_7_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_7=656,22
PRIMER_RIGHT_7=1626,20
PRIMER_LEFT_7_TM=59.864
PRIMER_RIGHT_7_TM=58.277
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.7000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=971
PRIMER_PAIR_8_PENALTY=3.884177
PRIMER_LEFT_8_PENALTY=2.422714
PRIMER_RIGHT_8_PENALTY=1.461462
PRIMER_LEFT_8_SEQUENCE=ATGTGTCATGGACCCGTATT
PRIMER_RIGHT_8_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_8=688,20
PRIMER_RIGHT_8=1617,20
PRIMER_LEFT_8_TM=57.577
PRIMER_RIGHT_8_TM=61.461
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=5.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=930
PRIMER_PAIR_9_PENALTY=3.972086
PRIMER_LEFT_9_PENALTY=1.453747
PRIMER_RIGHT_9_PENALTY=2.518339
PRIMER_LEFT_9_SEQUENCE=GATGTGTCATGGACCCGTATT
PRIMER_RIGHT_9_SEQUENCE=GGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_9=687,21
PRIMER_RIGHT_9=1616,20
PRIMER_LEFT_9_TM=59.546
PRIMER_RIGHT_9_TM=57.482
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.6000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=930
PRIMER_PAIR_10_PENALTY=4.145242
PRIMER_LEFT_10_PENALTY=2.422714
PRIMER_RIGHT_10_PENALTY=1.722528
PRIMER_LEFT_10_SEQUENCE=ATGTGTCATGGACCCGTATT
PRIMER_RIGHT_10_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_10=688,20
PRIMER_RIGHT_10=1626,20
PRIMER_LEFT_10_TM=57.577
PRIMER_RIGHT_10_TM=58.277
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.6000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=939
PRIMER_PAIR_11_PENALTY=4.219122
PRIMER_LEFT_11_PENALTY=2.757660
PRIMER_RIGHT_11_PENALTY=1.461462
PRIMER_LEFT_11_SEQUENCE=TGTCATGGACCCGTATTACACT
PRIMER_RIGHT_11_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_11=691,22
PRIMER_RIGHT_11=1617,20
PRIMER_LEFT_11_TM=59.242
PRIMER_RIGHT_11_TM=61.461
PRIMER_LEFT_11_GC_PERCENT=45.455
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=927
PRIMER_PAIR_12_PENALTY=4.480188
PRIMER_LEFT_12_PENALTY=2.757660
PRIMER_RIGHT_12_PENALTY=1.722528
PRIMER_LEFT_12_SEQUENCE=TGTCATGGACCCGTATTACACT
PRIMER_RIGHT_12_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_12=691,22
PRIMER_RIGHT_12=1626,20
PRIMER_LEFT_12_TM=59.242
PRIMER_RIGHT_12_TM=58.277
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.1000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=936
PRIMER_PAIR_13_PENALTY=4.507140
PRIMER_LEFT_13_PENALTY=1.988801
PRIMER_RIGHT_13_PENALTY=2.518339
PRIMER_LEFT_13_SEQUENCE=CGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_13_SEQUENCE=GGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_13=657,21
PRIMER_RIGHT_13=1616,20
PRIMER_LEFT_13_TM=59.011
PRIMER_RIGHT_13_TM=57.482
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.7000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=7.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=960
PRIMER_PAIR_14_PENALTY=4.515232
PRIMER_LEFT_14_PENALTY=3.053769
PRIMER_RIGHT_14_PENALTY=1.461462
PRIMER_LEFT_14_SEQUENCE=GTGTCATGGACCCGTATTACACT
PRIMER_RIGHT_14_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_14=690,23
PRIMER_RIGHT_14=1617,20
PRIMER_LEFT_14_TM=60.054
PRIMER_RIGHT_14_TM=61.461
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.1000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=928
PRIMER_PAIR_15_PENALTY=4.609245
PRIMER_LEFT_15_PENALTY=2.422714
PRIMER_RIGHT_15_PENALTY=2.186531
PRIMER_LEFT_15_SEQUENCE=ATGTGTCATGGACCCGTATT
PRIMER_RIGHT_15_SEQUENCE=CCGGTTTGGTTCGAATCTGT
PRIMER_LEFT_15=688,20
PRIMER_RIGHT_15=1618,20
PRIMER_LEFT_15_TM=57.577
PRIMER_RIGHT_15_TM=62.187
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=5.6000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=931
PRIMER_PAIR_16_PENALTY=4.654319
PRIMER_LEFT_16_PENALTY=2.135980
PRIMER_RIGHT_16_PENALTY=2.518339
PRIMER_LEFT_16_SEQUENCE=ACGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_16_SEQUENCE=GGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_16=656,22
PRIMER_RIGHT_16=1616,20
PRIMER_LEFT_16_TM=59.864
PRIMER_RIGHT_16_TM=57.482
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.7000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=961
PRIMER_PAIR_17_PENALTY=4.674084
PRIMER_LEFT_17_PENALTY=1.453747
PRIMER_RIGHT_17_PENALTY=3.220337
PRIMER_LEFT_17_SEQUENCE=GATGTGTCATGGACCCGTATT
PRIMER_RIGHT_17_SEQUENCE=CGGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_17=687,21
PRIMER_RIGHT_17=1617,21
PRIMER_LEFT_17_TM=59.546
PRIMER_RIGHT_17_TM=62.220
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.6000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=931
PRIMER_PAIR_18_PENALTY=4.776297
PRIMER_LEFT_18_PENALTY=3.053769
PRIMER_RIGHT_18_PENALTY=1.722528
PRIMER_LEFT_18_SEQUENCE=GTGTCATGGACCCGTATTACACT
PRIMER_RIGHT_18_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_18=690,23
PRIMER_RIGHT_18=1626,20
PRIMER_LEFT_18_TM=60.054
PRIMER_RIGHT_18_TM=58.277
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.1000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=937
PRIMER_PAIR_19_PENALTY=4.826476
PRIMER_LEFT_19_PENALTY=1.453747
PRIMER_RIGHT_19_PENALTY=3.372729
PRIMER_LEFT_19_SEQUENCE=GATGTGTCATGGACCCGTATT
PRIMER_RIGHT_19_SEQUENCE=GGTTTGGTTCGAATCTGTGTAG
PRIMER_LEFT_19=687,21
PRIMER_RIGHT_19=1616,22
PRIMER_LEFT_19_TM=59.546
PRIMER_RIGHT_19_TM=58.627
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=930
PRIMER_PAIR_20_PENALTY=4.856537
PRIMER_LEFT_20_PENALTY=3.134009
PRIMER_RIGHT_20_PENALTY=1.722528
PRIMER_LEFT_20_SEQUENCE=ACAGAGTCAGCCACGTAACA
PRIMER_RIGHT_20_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_20=660,20
PRIMER_RIGHT_20=1626,20
PRIMER_LEFT_20_TM=56.866
PRIMER_RIGHT_20_TM=58.277
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.0000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=967
PRIMER_PAIR_21_PENALTY=4.941054
PRIMER_LEFT_21_PENALTY=2.422714
PRIMER_RIGHT_21_PENALTY=2.518339
PRIMER_LEFT_21_SEQUENCE=ATGTGTCATGGACCCGTATT
PRIMER_RIGHT_21_SEQUENCE=GGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_21=688,20
PRIMER_RIGHT_21=1616,20
PRIMER_LEFT_21_TM=57.577
PRIMER_RIGHT_21_TM=57.482
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=5.6000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=929
PRIMER_PAIR_22_PENALTY=4.944191
PRIMER_LEFT_22_PENALTY=2.757660
PRIMER_RIGHT_22_PENALTY=2.186531
PRIMER_LEFT_22_SEQUENCE=TGTCATGGACCCGTATTACACT
PRIMER_RIGHT_22_SEQUENCE=CCGGTTTGGTTCGAATCTGT
PRIMER_LEFT_22=691,22
PRIMER_RIGHT_22=1618,20
PRIMER_LEFT_22_TM=59.242
PRIMER_RIGHT_22_TM=62.187
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.1000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=928
PRIMER_PAIR_23_PENALTY=4.978030
PRIMER_LEFT_23_PENALTY=3.255503
PRIMER_RIGHT_23_PENALTY=1.722528
PRIMER_LEFT_23_SEQUENCE=GGCTACGTACAGAGTCAGCCAC
PRIMER_RIGHT_23_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_23=652,22
PRIMER_RIGHT_23=1626,20
PRIMER_LEFT_23_TM=61.256
PRIMER_RIGHT_23_TM=58.277
PRIMER_LEFT_23_GC_PERCENT=59.091
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=975
PRIMER_PAIR_24_PENALTY=5.055295
PRIMER_LEFT_24_PENALTY=3.593833
PRIMER_RIGHT_24_PENALTY=1.461462
PRIMER_LEFT_24_SEQUENCE=TCATGGACCCGTATTACACTC
PRIMER_RIGHT_24_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_24=693,21
PRIMER_RIGHT_24=1617,20
PRIMER_LEFT_24_TM=57.406
PRIMER_RIGHT_24_TM=61.461
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=925
PRIMER_PAIR_25_PENALTY=5.069132
PRIMER_LEFT_25_PENALTY=3.346604
PRIMER_RIGHT_25_PENALTY=1.722528
PRIMER_LEFT_25_SEQUENCE=GCTACGTACAGAGTCAGCCA
PRIMER_RIGHT_25_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_25=653,20
PRIMER_RIGHT_25=1626,20
PRIMER_LEFT_25_TM=56.653
PRIMER_RIGHT_25_TM=58.277
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.7000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=974
PRIMER_PAIR_26_PENALTY=5.076649
PRIMER_LEFT_26_PENALTY=3.354121
PRIMER_RIGHT_26_PENALTY=1.722528
PRIMER_LEFT_26_SEQUENCE=GCTACGTACAGAGTCAGCCAC
PRIMER_RIGHT_26_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_26=653,21
PRIMER_RIGHT_26=1626,20
PRIMER_LEFT_26_TM=57.646
PRIMER_RIGHT_26_TM=58.277
PRIMER_LEFT_26_GC_PERCENT=57.143
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=974
PRIMER_PAIR_27_PENALTY=5.142409
PRIMER_LEFT_27_PENALTY=3.680946
PRIMER_RIGHT_27_PENALTY=1.461462
PRIMER_LEFT_27_SEQUENCE=GTCATGGACCCGTATTACACTC
PRIMER_RIGHT_27_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_27=692,22
PRIMER_RIGHT_27=1617,20
PRIMER_LEFT_27_TM=58.319
PRIMER_RIGHT_27_TM=61.461
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=926
PRIMER_PAIR_28_PENALTY=5.209138
PRIMER_LEFT_28_PENALTY=1.988801
PRIMER_RIGHT_28_PENALTY=3.220337
PRIMER_LEFT_28_SEQUENCE=CGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_28_SEQUENCE=CGGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_28=657,21
PRIMER_RIGHT_28=1617,21
PRIMER_LEFT_28_TM=59.011
PRIMER_RIGHT_28_TM=62.220
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.7000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=961
PRIMER_PAIR_29_PENALTY=5.240301
PRIMER_LEFT_29_PENALTY=3.053769
PRIMER_RIGHT_29_PENALTY=2.186531
PRIMER_LEFT_29_SEQUENCE=GTGTCATGGACCCGTATTACACT
PRIMER_RIGHT_29_SEQUENCE=CCGGTTTGGTTCGAATCTGT
PRIMER_LEFT_29=690,23
PRIMER_RIGHT_29=1618,20
PRIMER_LEFT_29_TM=60.054
PRIMER_RIGHT_29_TM=62.187
PRIMER_LEFT_29_GC_PERCENT=47.826
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.1000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=929
PRIMER_PAIR_30_PENALTY=5.275999
PRIMER_LEFT_30_PENALTY=2.757660
PRIMER_RIGHT_30_PENALTY=2.518339
PRIMER_LEFT_30_SEQUENCE=TGTCATGGACCCGTATTACACT
PRIMER_RIGHT_30_SEQUENCE=GGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_30=691,22
PRIMER_RIGHT_30=1616,20
PRIMER_LEFT_30_TM=59.242
PRIMER_RIGHT_30_TM=57.482
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.1000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=926
PRIMER_PAIR_31_PENALTY=5.316360
PRIMER_LEFT_31_PENALTY=3.593833
PRIMER_RIGHT_31_PENALTY=1.722528
PRIMER_LEFT_31_SEQUENCE=TCATGGACCCGTATTACACTC
PRIMER_RIGHT_31_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_31=693,21
PRIMER_RIGHT_31=1626,20
PRIMER_LEFT_31_TM=57.406
PRIMER_RIGHT_31_TM=58.277
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=7.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=934
PRIMER_PAIR_32_PENALTY=5.356317
PRIMER_LEFT_32_PENALTY=2.135980
PRIMER_RIGHT_32_PENALTY=3.220337
PRIMER_LEFT_32_SEQUENCE=ACGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_32_SEQUENCE=CGGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_32=656,22
PRIMER_RIGHT_32=1617,21
PRIMER_LEFT_32_TM=59.864
PRIMER_RIGHT_32_TM=62.220
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.7000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=962
PRIMER_PAIR_33_PENALTY=5.361530
PRIMER_LEFT_33_PENALTY=1.988801
PRIMER_RIGHT_33_PENALTY=3.372729
PRIMER_LEFT_33_SEQUENCE=CGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_33_SEQUENCE=GGTTTGGTTCGAATCTGTGTAG
PRIMER_LEFT_33=657,21
PRIMER_RIGHT_33=1616,22
PRIMER_LEFT_33_TM=59.011
PRIMER_RIGHT_33_TM=58.627
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.7000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=960
PRIMER_PAIR_34_PENALTY=5.403474
PRIMER_LEFT_34_PENALTY=3.680946
PRIMER_RIGHT_34_PENALTY=1.722528
PRIMER_LEFT_34_SEQUENCE=GTCATGGACCCGTATTACACTC
PRIMER_RIGHT_34_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_34=692,22
PRIMER_RIGHT_34=1626,20
PRIMER_LEFT_34_TM=58.319
PRIMER_RIGHT_34_TM=58.277
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=7.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=935
PRIMER_PAIR_35_PENALTY=5.455789
PRIMER_LEFT_35_PENALTY=3.994327
PRIMER_RIGHT_35_PENALTY=1.461462
PRIMER_LEFT_35_SEQUENCE=TACAGAGTCAGCCACGTAACA
PRIMER_RIGHT_35_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_35=659,21
PRIMER_RIGHT_35=1617,20
PRIMER_LEFT_35_TM=57.006
PRIMER_RIGHT_35_TM=61.461
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.0000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=7.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=959
PRIMER_PAIR_36_PENALTY=5.490610
PRIMER_LEFT_36_PENALTY=4.029148
PRIMER_RIGHT_36_PENALTY=1.461462
PRIMER_LEFT_36_SEQUENCE=TGTCATGGACCCGTATTACACTC
PRIMER_RIGHT_36_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_36=691,23
PRIMER_RIGHT_36=1617,20
PRIMER_LEFT_36_TM=61.029
PRIMER_RIGHT_36_TM=61.461
PRIMER_LEFT_36_GC_PERCENT=47.826
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=927
PRIMER_PAIR_37_PENALTY=5.492899
PRIMER_LEFT_37_PENALTY=4.031436
PRIMER_RIGHT_37_PENALTY=1.461462
PRIMER_LEFT_37_SEQUENCE=GTACAGAGTCAGCCACGTAACA
PRIMER_RIGHT_37_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_37=658,22
PRIMER_RIGHT_37=1617,20
PRIMER_LEFT_37_TM=57.969
PRIMER_RIGHT_37_TM=61.461
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.0000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=7.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=960
PRIMER_PAIR_38_PENALTY=5.508710
PRIMER_LEFT_38_PENALTY=2.135980
PRIMER_RIGHT_38_PENALTY=3.372729
PRIMER_LEFT_38_SEQUENCE=ACGTACAGAGTCAGCCACGTAA
PRIMER_RIGHT_38_SEQUENCE=GGTTTGGTTCGAATCTGTGTAG
PRIMER_LEFT_38=656,22
PRIMER_RIGHT_38=1616,22
PRIMER_LEFT_38_TM=59.864
PRIMER_RIGHT_38_TM=58.627
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.7000
PRIMER_RIGHT_38_END_STABILITY=5.7000
PRIMER_PAIR_38_COMPL_ANY=7.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=961
PRIMER_PAIR_39_PENALTY=5.572109
PRIMER_LEFT_39_PENALTY=3.053769
PRIMER_RIGHT_39_PENALTY=2.518339
PRIMER_LEFT_39_SEQUENCE=GTGTCATGGACCCGTATTACACT
PRIMER_RIGHT_39_SEQUENCE=GGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_39=690,23
PRIMER_RIGHT_39=1616,20
PRIMER_LEFT_39_TM=60.054
PRIMER_RIGHT_39_TM=57.482
PRIMER_LEFT_39_GC_PERCENT=47.826
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.1000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=927
PRIMER_PAIR_40_PENALTY=5.643051
PRIMER_LEFT_40_PENALTY=2.422714
PRIMER_RIGHT_40_PENALTY=3.220337
PRIMER_LEFT_40_SEQUENCE=ATGTGTCATGGACCCGTATT
PRIMER_RIGHT_40_SEQUENCE=CGGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_40=688,20
PRIMER_RIGHT_40=1617,21
PRIMER_LEFT_40_TM=57.577
PRIMER_RIGHT_40_TM=62.220
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.6000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=930
PRIMER_PAIR_41_PENALTY=5.716855
PRIMER_LEFT_41_PENALTY=3.994327
PRIMER_RIGHT_41_PENALTY=1.722528
PRIMER_LEFT_41_SEQUENCE=TACAGAGTCAGCCACGTAACA
PRIMER_RIGHT_41_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_41=659,21
PRIMER_RIGHT_41=1626,20
PRIMER_LEFT_41_TM=57.006
PRIMER_RIGHT_41_TM=58.277
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.0000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=968
PRIMER_PAIR_42_PENALTY=5.751675
PRIMER_LEFT_42_PENALTY=4.029148
PRIMER_RIGHT_42_PENALTY=1.722528
PRIMER_LEFT_42_SEQUENCE=TGTCATGGACCCGTATTACACTC
PRIMER_RIGHT_42_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_42=691,23
PRIMER_RIGHT_42=1626,20
PRIMER_LEFT_42_TM=61.029
PRIMER_RIGHT_42_TM=58.277
PRIMER_LEFT_42_GC_PERCENT=47.826
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=936
PRIMER_PAIR_43_PENALTY=5.753964
PRIMER_LEFT_43_PENALTY=4.031436
PRIMER_RIGHT_43_PENALTY=1.722528
PRIMER_LEFT_43_SEQUENCE=GTACAGAGTCAGCCACGTAACA
PRIMER_RIGHT_43_SEQUENCE=GGTTTAATCCGGTTTGGTTC
PRIMER_LEFT_43=658,22
PRIMER_RIGHT_43=1626,20
PRIMER_LEFT_43_TM=57.969
PRIMER_RIGHT_43_TM=58.277
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.0000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=969
PRIMER_PAIR_44_PENALTY=5.780364
PRIMER_LEFT_44_PENALTY=3.593833
PRIMER_RIGHT_44_PENALTY=2.186531
PRIMER_LEFT_44_SEQUENCE=TCATGGACCCGTATTACACTC
PRIMER_RIGHT_44_SEQUENCE=CCGGTTTGGTTCGAATCTGT
PRIMER_LEFT_44=693,21
PRIMER_RIGHT_44=1618,20
PRIMER_LEFT_44_TM=57.406
PRIMER_RIGHT_44_TM=62.187
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=926
PRIMER_PAIR_45_PENALTY=5.795444
PRIMER_LEFT_45_PENALTY=2.422714
PRIMER_RIGHT_45_PENALTY=3.372729
PRIMER_LEFT_45_SEQUENCE=ATGTGTCATGGACCCGTATT
PRIMER_RIGHT_45_SEQUENCE=GGTTTGGTTCGAATCTGTGTAG
PRIMER_LEFT_45=688,20
PRIMER_RIGHT_45=1616,22
PRIMER_LEFT_45_TM=57.577
PRIMER_RIGHT_45_TM=58.627
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=5.6000
PRIMER_RIGHT_45_END_STABILITY=5.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=929
PRIMER_PAIR_46_PENALTY=5.867477
PRIMER_LEFT_46_PENALTY=3.680946
PRIMER_RIGHT_46_PENALTY=2.186531
PRIMER_LEFT_46_SEQUENCE=GTCATGGACCCGTATTACACTC
PRIMER_RIGHT_46_SEQUENCE=CCGGTTTGGTTCGAATCTGT
PRIMER_LEFT_46=692,22
PRIMER_RIGHT_46=1618,20
PRIMER_LEFT_46_TM=58.319
PRIMER_RIGHT_46_TM=62.187
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=927
PRIMER_PAIR_47_PENALTY=5.977997
PRIMER_LEFT_47_PENALTY=2.757660
PRIMER_RIGHT_47_PENALTY=3.220337
PRIMER_LEFT_47_SEQUENCE=TGTCATGGACCCGTATTACACT
PRIMER_RIGHT_47_SEQUENCE=CGGTTTGGTTCGAATCTGTGT
PRIMER_LEFT_47=691,22
PRIMER_RIGHT_47=1617,21
PRIMER_LEFT_47_TM=59.242
PRIMER_RIGHT_47_TM=62.220
PRIMER_LEFT_47_GC_PERCENT=45.455
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.1000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=927
PRIMER_PAIR_48_PENALTY=6.022968
PRIMER_LEFT_48_PENALTY=1.204438
PRIMER_RIGHT_48_PENALTY=4.818530
PRIMER_LEFT_48_SEQUENCE=TCGATGCAATTCGTCAAACA
PRIMER_RIGHT_48_SEQUENCE=TGCAGAATAAGAGGAAGGAA
PRIMER_LEFT_48=238,20
PRIMER_RIGHT_48=1205,20
PRIMER_LEFT_48_TM=61.204
PRIMER_RIGHT_48_TM=55.181
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=968
PRIMER_PAIR_49_PENALTY=6.066893
PRIMER_LEFT_49_PENALTY=4.605431
PRIMER_RIGHT_49_PENALTY=1.461462
PRIMER_LEFT_49_SEQUENCE=GTCATGGACCCGTATTACACT
PRIMER_RIGHT_49_SEQUENCE=CGGTTTGGTTCGAATCTGTG
PRIMER_LEFT_49=692,21
PRIMER_RIGHT_49=1617,20
PRIMER_LEFT_49_TM=56.395
PRIMER_RIGHT_49_TM=61.461
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.1000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=926
=
SEQUENCE_ID=MAGIC.329_4_4692194_4692593_edi-0_wil-2_65086_20282_TRUE
SEQUENCE_TARGET=950,499
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGAATATACCTGNATATTNTGCAGNGGAANTATGAANCATGNTAATTCGNNNNTGGTTTAGNANTCCAAGNATTGTGCCTCTACTGGTANCCACACGCATNCAAANTNGATCAANCCCNNAATGCTANTTCCTTTGAGATCTAATCTCAAAACTTAGAAAAACAATNTTTCTCTTTGTTTTTATGNGTTGAAGATAATTTATCAAATTTTNAGAGAACATNACCGTAATAACNGTAACCGTGGTCATGCCTANNTNTNNNNNNTATTNATCATNCCAAAATAGACATACCATCATCAGTCGTTAAAACAANTTGANAANTTATCNNCGACTTTCTTTCTCTTCTCTCTCNTAATCCTTNAGAGAGAGAATGTCTCCGATGGTTGTTTTTGGTGGCAACGACATGTTTCTTCACTTGCGTTGGCTCTCATATGCCCAACACANTTGGTTACCTCAACGACGGTGACTGGTTTTCTCGTCCGNTTTNGGTGGATTCATCTCCGCCGTTGGNNGGCTCCCGTCCGGAATTGTCTGGCTCACATAGTAGAGNTGTCCGGCTTTCGTCCAGCACCGTCGCGTTTCCACGGTGGCATGTGGCTAGCTTTCTCCTATGCATCCTCTTTCCTTTGTTTATCTGTGTTTTTACTTTTGTTTGCNTTTAATAATCTCACATACATGGGTTGGTAATGACANTGTAAGTAATCTAGCTTTNTACTGTTNTGTTCCGATCTATTTCATGNTGAGACTTAATTGCAGAAATNAAATAAGAGATCTNNGAATGANGATGAAGATAAGAAGTGGAATCCGTCGCTTATTAATCATTGCCAATCTGCTGATGTATCTTTAATTGGAGCCTCTTTACTNTCTACCGGTGAGAAANCCTTAAGAATCCATGTTAGATAGTCCTATCANCANTGATTCTGTCACCGGAANTGAAGCTCCNACGGTGGAGGTTGAAGTCGGTTTGCTTAAGATTGCTCATCTGACACGTGGTTATGTCGACGTGTGTTTCAGGATTGAGTCAAATCCAGTTTTCTTCTTCTGTGAGCAAGATGAAATCTTCTGATGGGCCTANNNNNNCNANNAAACATTTTGTAATCNTAAGGGNTCAAAAACGGNTGCATCTCAAAAGTCATAATGCATCACTTTTATAATAGGTTCACNNAACCTAATGCATATAAAGTCAATGGGCCAAGCCCAATCCATGCAANGTNACTTTCTNTACNAGAATAAACCACATATTCGTATTAGTGCACATATGTATATTTTATATACATATATAACATTATTTATTTAAGTGCTCAAATACAAATATAAGTATATCTATATATNNCATATATATTATATACTTTTACTTTAATTTATATATNATATATATANANNNNNAATCTTTTAACCTAATATTAGTTTAATCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAACTCATAGATTATAAGCGNTCTCTAGCAAAACATTATAACCATCTAATNNNNNNNNNNNNNNNNNNNNNNNNNNTANAACTTTAACTAGAGTNNAGTACAAACGATTTCAAGATATTCCCAACATTATTTAGNTTTGATGATGTGTACAATGTCTNGAGTNNTGTGTGTGTGGATTGGTAGANNNNNNNNNNNNNNNTTCCCTGATAGTTTTTCCCCTTTCCTCTTGNNTTATTTGTTTTATAGGTGTCTTCTTTGTATCGCACATGTACGTGCTGATGGGCNGTAGAATCTATCNNGGCACGGAAGNAAAAATACGATTAGGAAGTTTTACGGTAAGACTAAGAAGCCCTTTTGATGCCANTGNTTTTTTTTTATTAGTGGGTTAATCTTACAACATGGCNNTGGCTAAGTGCAAGTGTTATATTACCGTTACTTGAGAGACTTCATATCAACTTTGTTGACNTATTGGATGAAAGCTTGTGGTATCCGANTCCAAAAGCCATCACCTAAAAGCAGTNTGACATTGAAGGTAGCAGATTTATGAATAGCATTGACTTGGAAAGAGAAGACGAATGCGCGANTTGCTTAGAGCCTTGCACCAAAATGGTGTTGCCAAAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9581907_9582151_kn-0_po-0_92592_210844_TRUE
SEQUENCE_TARGET=950,344
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANACATAAANTAACCTGAGAATNGCTTCGCANATAAAGACATTGTTGTANAGTTTGTCAAGCTTGATGGCTGGAAGTGACGCCACCATGTCCATGAANTTTTTAGCAATGATCTTAACTTGCGGCATCTTCACCAATTGGATTCCCTTACTCACTGGACCGGTTCTAAAAGNGCACAGTTTCCACGAAATTAGGGTTTANGCCACACAGCGATCAGCTCNAANCAAACCTAGTTAACTAAAGCATCTCCTNAAAAAAAACAAATCCAAAACAGTNCTNAANAAGAACTCCANAAAACNAATAAGGAAGGANTTAAAGTTTCNGGTTCNANTTTGAGAANAANACAGAATTGAATTTANNGTTTANGTTNTCTCTTNNNTCGANGANCTCNNNNNNNNNNNNNNNNNNNNNNTTANTACCCAGAATCNAAGAAAACTGANGNAATCGATCGAAGAGGATGNCCAAGATAANNNNANNTTTAATGGGCCTNNNNNATACTACTAATGGGCCTNTCTNTNTAACAAAAATCGCGCCAATTTGTTTTCATGAAAATGGTTNNNTNGGGAGAGATATATGCTTGANTGACCAAAATGTAAANAAAAAAAGCNTANNNNNNNNNNNNNNNNNNNNTGATAAATTGATTAGTACTGATTTANTCCATNNANATCNAACTCATACNANNCATTACAATCTTCTTANNGAGNCACAATACANANATTATATATNAATCTNCTACGATTATTTATATTATCATCNNNNATTATATTGTCCTAGAGGGCATTATTTAAATATATNAGCCATTGCATANAGAAACCATNATNAATACTATATGNTANAAACACACTGATGAAAAGTGAATCAATTAAGTAAGTAGAATGTTGGAGTCGTAGGTGGCTCCGGCNNTCCAATCAGCGGGAATAGCGTTNGGNGATTGGATCCAANTGANTCCNGCNCTTCCGTAGACTAAGAACCTCAATGTGAGAGTGCCTCTAGGTGGNTTCTGCAAATCATGAACCGCTCCAAACACTCTTCTCATACGTCTCCACTCTTTGCAATCCTCCTAAAATCATACATATACATGCATACCATNTAATAACGTTTAACTTTGTTTCACAAAATCATCTAAGAGTAAACTATATATTTTGATCCTCAAAAACANATATCTAAGTTTCTNAACATCTTTGATTAAATTTACCNGCCAGACTTCAACGGCGAGNATGTCATTAACACCACCAACGTACAAGACAAGGATGGCAAGATAATGAGGATTNTAGCTTTTCTCATGGATCTTATANACNAGATTATACCCTGCGTATCGGCAAGGGATCCTTTGGTACTCAACGTTGACTACACCGAAGGAGTAGAGCTGATTTTCTGTGCCGGGTCGCGCCATACGTCCGTACGCCTTAGGGCTTAAGATGAAGTCCGTNCCATCTCCTTCTCCNGAGTCCGTAGCCACTACGTATACTCCTTCCTCACTGCAGTGTGGTGGNATCTTGCACCTCACCNTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTTTAGNNNNTANTGACATAGNATANACTATNATAATATTTGATGAAANATTNATTGAGATAAGTAGATAANNNTATANAANAACCTGGTAACAAGCACCACAGCCAGTTCCATTGTTCCATAGNCGCCNTGAAACACCACTCACTTCACCGTTATTGATATCTCTTCCAAATTCTCCATACCCACAATGTCCNCCTGCANCATCNAAAAATTAATTNANTATNNNNNNNNNNNCGACAAANNTTNTNTATATTCTCATAATNGAAAGTTNTNATTACTAATTACGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAAAGAATTAAGGTACACGANNTATATAGCAATAACANTTTTAATACGTACGAGGATTTGCTTTGCAATCGGGGCTGCCATAATAAGTAGCTCTAGAGTTAACAAAATCGTCGGATAGNCAAAGGAGAGGCAAAANGANAATGACTTGAACAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.703593
PRIMER_LEFT_0_PENALTY=0.272667
PRIMER_RIGHT_0_PENALTY=0.430926
PRIMER_LEFT_0_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_0_SEQUENCE=ACAATGGAACTGGCTGTGGT
PRIMER_LEFT_0=925,20
PRIMER_RIGHT_0=1717,20
PRIMER_LEFT_0_TM=59.727
PRIMER_RIGHT_0_TM=60.431
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=793
PRIMER_PAIR_1_PENALTY=0.862570
PRIMER_LEFT_1_PENALTY=0.272667
PRIMER_RIGHT_1_PENALTY=0.589903
PRIMER_LEFT_1_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_1_SEQUENCE=ATGGAACAATGGAACTGGCT
PRIMER_LEFT_1=925,20
PRIMER_RIGHT_1=1722,20
PRIMER_LEFT_1_TM=59.727
PRIMER_RIGHT_1_TM=59.410
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=798
PRIMER_PAIR_2_PENALTY=1.026520
PRIMER_LEFT_2_PENALTY=0.272667
PRIMER_RIGHT_2_PENALTY=0.753853
PRIMER_LEFT_2_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_2_SEQUENCE=AACTGGCTGTGGTGCTTGTT
PRIMER_LEFT_2=925,20
PRIMER_RIGHT_2=1710,20
PRIMER_LEFT_2_TM=59.727
PRIMER_RIGHT_2_TM=60.754
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=786
PRIMER_PAIR_3_PENALTY=1.133831
PRIMER_LEFT_3_PENALTY=0.272667
PRIMER_RIGHT_3_PENALTY=0.861164
PRIMER_LEFT_3_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_3_SEQUENCE=GAACAATGGAACTGGCTGTG
PRIMER_LEFT_3=925,20
PRIMER_RIGHT_3=1719,20
PRIMER_LEFT_3_TM=59.727
PRIMER_RIGHT_3_TM=59.139
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=795
PRIMER_PAIR_4_PENALTY=1.142110
PRIMER_LEFT_4_PENALTY=0.711184
PRIMER_RIGHT_4_PENALTY=0.430926
PRIMER_LEFT_4_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_4_SEQUENCE=ACAATGGAACTGGCTGTGGT
PRIMER_LEFT_4=924,20
PRIMER_RIGHT_4=1717,20
PRIMER_LEFT_4_TM=60.711
PRIMER_RIGHT_4_TM=60.431
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=794
PRIMER_PAIR_5_PENALTY=1.227064
PRIMER_LEFT_5_PENALTY=0.272667
PRIMER_RIGHT_5_PENALTY=0.954397
PRIMER_LEFT_5_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_5_SEQUENCE=AACAATGGAACTGGCTGTGG
PRIMER_LEFT_5=925,20
PRIMER_RIGHT_5=1718,20
PRIMER_LEFT_5_TM=59.727
PRIMER_RIGHT_5_TM=60.954
PRIMER_LEFT_5_GC_PERCENT=60.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=794
PRIMER_PAIR_6_PENALTY=1.301087
PRIMER_LEFT_6_PENALTY=0.711184
PRIMER_RIGHT_6_PENALTY=0.589903
PRIMER_LEFT_6_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_6_SEQUENCE=ATGGAACAATGGAACTGGCT
PRIMER_LEFT_6=924,20
PRIMER_RIGHT_6=1722,20
PRIMER_LEFT_6_TM=60.711
PRIMER_RIGHT_6_TM=59.410
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=799
PRIMER_PAIR_7_PENALTY=1.449534
PRIMER_LEFT_7_PENALTY=0.272667
PRIMER_RIGHT_7_PENALTY=1.176867
PRIMER_LEFT_7_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_7_SEQUENCE=TGTGGGTATGGAGAATTTGGA
PRIMER_LEFT_7=925,20
PRIMER_RIGHT_7=1787,21
PRIMER_LEFT_7_TM=59.727
PRIMER_RIGHT_7_TM=60.177
PRIMER_LEFT_7_GC_PERCENT=60.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=863
PRIMER_PAIR_8_PENALTY=1.465037
PRIMER_LEFT_8_PENALTY=0.711184
PRIMER_RIGHT_8_PENALTY=0.753853
PRIMER_LEFT_8_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_8_SEQUENCE=AACTGGCTGTGGTGCTTGTT
PRIMER_LEFT_8=924,20
PRIMER_RIGHT_8=1710,20
PRIMER_LEFT_8_TM=60.711
PRIMER_RIGHT_8_TM=60.754
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=787
PRIMER_PAIR_9_PENALTY=1.572348
PRIMER_LEFT_9_PENALTY=0.711184
PRIMER_RIGHT_9_PENALTY=0.861164
PRIMER_LEFT_9_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_9_SEQUENCE=GAACAATGGAACTGGCTGTG
PRIMER_LEFT_9=924,20
PRIMER_RIGHT_9=1719,20
PRIMER_LEFT_9_TM=60.711
PRIMER_RIGHT_9_TM=59.139
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=796
PRIMER_PAIR_10_PENALTY=1.592628
PRIMER_LEFT_10_PENALTY=0.272667
PRIMER_RIGHT_10_PENALTY=1.319961
PRIMER_LEFT_10_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_10_SEQUENCE=GAACTGGCTGTGGTGCTTGT
PRIMER_LEFT_10=925,20
PRIMER_RIGHT_10=1711,20
PRIMER_LEFT_10_TM=59.727
PRIMER_RIGHT_10_TM=61.320
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=787
PRIMER_PAIR_11_PENALTY=1.601764
PRIMER_LEFT_11_PENALTY=0.272667
PRIMER_RIGHT_11_PENALTY=1.329097
PRIMER_LEFT_11_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_11_SEQUENCE=TTGTGGGTATGGAGAATTTGG
PRIMER_LEFT_11=925,20
PRIMER_RIGHT_11=1788,21
PRIMER_LEFT_11_TM=59.727
PRIMER_RIGHT_11_TM=59.671
PRIMER_LEFT_11_GC_PERCENT=60.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=864
PRIMER_PAIR_12_PENALTY=1.611072
PRIMER_LEFT_12_PENALTY=0.272667
PRIMER_RIGHT_12_PENALTY=1.338405
PRIMER_LEFT_12_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_12_SEQUENCE=GGACATTGTGGGTATGGAGAA
PRIMER_LEFT_12=925,20
PRIMER_RIGHT_12=1793,21
PRIMER_LEFT_12_TM=59.727
PRIMER_RIGHT_12_TM=59.662
PRIMER_LEFT_12_GC_PERCENT=60.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=869
PRIMER_PAIR_13_PENALTY=1.652018
PRIMER_LEFT_13_PENALTY=0.272667
PRIMER_RIGHT_13_PENALTY=1.379351
PRIMER_LEFT_13_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_13_SEQUENCE=CGGTGAAGTGAGTGGTGTTT
PRIMER_LEFT_13=925,20
PRIMER_RIGHT_13=1752,20
PRIMER_LEFT_13_TM=59.727
PRIMER_RIGHT_13_TM=58.621
PRIMER_LEFT_13_GC_PERCENT=60.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=828
PRIMER_PAIR_14_PENALTY=1.665581
PRIMER_LEFT_14_PENALTY=0.711184
PRIMER_RIGHT_14_PENALTY=0.954397
PRIMER_LEFT_14_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_14_SEQUENCE=AACAATGGAACTGGCTGTGG
PRIMER_LEFT_14=924,20
PRIMER_RIGHT_14=1718,20
PRIMER_LEFT_14_TM=60.711
PRIMER_RIGHT_14_TM=60.954
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=795
PRIMER_PAIR_15_PENALTY=1.739811
PRIMER_LEFT_15_PENALTY=0.272667
PRIMER_RIGHT_15_PENALTY=1.467144
PRIMER_LEFT_15_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_15_SEQUENCE=ACGGTGAAGTGAGTGGTGTTT
PRIMER_LEFT_15=925,20
PRIMER_RIGHT_15=1753,21
PRIMER_LEFT_15_TM=59.727
PRIMER_RIGHT_15_TM=59.533
PRIMER_LEFT_15_GC_PERCENT=60.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=829
PRIMER_PAIR_16_PENALTY=1.739811
PRIMER_LEFT_16_PENALTY=0.272667
PRIMER_RIGHT_16_PENALTY=1.467144
PRIMER_LEFT_16_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_16_SEQUENCE=AACGGTGAAGTGAGTGGTGTT
PRIMER_LEFT_16=925,20
PRIMER_RIGHT_16=1754,21
PRIMER_LEFT_16_TM=59.727
PRIMER_RIGHT_16_TM=59.533
PRIMER_LEFT_16_GC_PERCENT=60.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=830
PRIMER_PAIR_17_PENALTY=1.829435
PRIMER_LEFT_17_PENALTY=0.272667
PRIMER_RIGHT_17_PENALTY=1.556768
PRIMER_LEFT_17_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_17_SEQUENCE=TATGGAACAATGGAACTGGCT
PRIMER_LEFT_17=925,20
PRIMER_RIGHT_17=1723,21
PRIMER_LEFT_17_TM=59.727
PRIMER_RIGHT_17_TM=59.443
PRIMER_LEFT_17_GC_PERCENT=60.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=799
PRIMER_PAIR_18_PENALTY=1.867655
PRIMER_LEFT_18_PENALTY=0.272667
PRIMER_RIGHT_18_PENALTY=1.594988
PRIMER_LEFT_18_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_18_SEQUENCE=CGGTGAAGTGAGTGGTGTTTC
PRIMER_LEFT_18=925,20
PRIMER_RIGHT_18=1752,21
PRIMER_LEFT_18_TM=59.727
PRIMER_RIGHT_18_TM=60.595
PRIMER_LEFT_18_GC_PERCENT=60.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=828
PRIMER_PAIR_19_PENALTY=1.888051
PRIMER_LEFT_19_PENALTY=0.711184
PRIMER_RIGHT_19_PENALTY=1.176867
PRIMER_LEFT_19_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_19_SEQUENCE=TGTGGGTATGGAGAATTTGGA
PRIMER_LEFT_19=924,20
PRIMER_RIGHT_19=1787,21
PRIMER_LEFT_19_TM=60.711
PRIMER_RIGHT_19_TM=60.177
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=2.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=864
PRIMER_PAIR_20_PENALTY=1.967832
PRIMER_LEFT_20_PENALTY=1.536905
PRIMER_RIGHT_20_PENALTY=0.430926
PRIMER_LEFT_20_SEQUENCE=GAATGTTGGAGTCGTAGGTGG
PRIMER_RIGHT_20_SEQUENCE=ACAATGGAACTGGCTGTGGT
PRIMER_LEFT_20=921,21
PRIMER_RIGHT_20=1717,20
PRIMER_LEFT_20_TM=59.463
PRIMER_RIGHT_20_TM=60.431
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=797
PRIMER_PAIR_21_PENALTY=2.002439
PRIMER_LEFT_21_PENALTY=0.272667
PRIMER_RIGHT_21_PENALTY=1.729772
PRIMER_LEFT_21_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_21_SEQUENCE=TGTGGGTATGGAGAATTTGG
PRIMER_LEFT_21=925,20
PRIMER_RIGHT_21=1787,20
PRIMER_LEFT_21_TM=59.727
PRIMER_RIGHT_21_TM=58.270
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=863
PRIMER_PAIR_22_PENALTY=2.031145
PRIMER_LEFT_22_PENALTY=0.711184
PRIMER_RIGHT_22_PENALTY=1.319961
PRIMER_LEFT_22_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_22_SEQUENCE=GAACTGGCTGTGGTGCTTGT
PRIMER_LEFT_22=924,20
PRIMER_RIGHT_22=1711,20
PRIMER_LEFT_22_TM=60.711
PRIMER_RIGHT_22_TM=61.320
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=788
PRIMER_PAIR_23_PENALTY=2.040281
PRIMER_LEFT_23_PENALTY=0.711184
PRIMER_RIGHT_23_PENALTY=1.329097
PRIMER_LEFT_23_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_23_SEQUENCE=TTGTGGGTATGGAGAATTTGG
PRIMER_LEFT_23=924,20
PRIMER_RIGHT_23=1788,21
PRIMER_LEFT_23_TM=60.711
PRIMER_RIGHT_23_TM=59.671
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=865
PRIMER_PAIR_24_PENALTY=2.049589
PRIMER_LEFT_24_PENALTY=0.711184
PRIMER_RIGHT_24_PENALTY=1.338405
PRIMER_LEFT_24_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_24_SEQUENCE=GGACATTGTGGGTATGGAGAA
PRIMER_LEFT_24=924,20
PRIMER_RIGHT_24=1793,21
PRIMER_LEFT_24_TM=60.711
PRIMER_RIGHT_24_TM=59.662
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=870
PRIMER_PAIR_25_PENALTY=2.071510
PRIMER_LEFT_25_PENALTY=0.272667
PRIMER_RIGHT_25_PENALTY=1.798843
PRIMER_LEFT_25_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_25_SEQUENCE=GGACATTGTGGGTATGGAGA
PRIMER_LEFT_25=925,20
PRIMER_RIGHT_25=1793,20
PRIMER_LEFT_25_TM=59.727
PRIMER_RIGHT_25_TM=58.201
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=869
PRIMER_PAIR_26_PENALTY=2.085841
PRIMER_LEFT_26_PENALTY=0.272667
PRIMER_RIGHT_26_PENALTY=1.813174
PRIMER_LEFT_26_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_26_SEQUENCE=TCAGCTCTACTCCTTCGGTG
PRIMER_LEFT_26=925,20
PRIMER_RIGHT_26=1417,20
PRIMER_LEFT_26_TM=59.727
PRIMER_RIGHT_26_TM=58.187
PRIMER_LEFT_26_GC_PERCENT=60.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=493
PRIMER_PAIR_27_PENALTY=2.090535
PRIMER_LEFT_27_PENALTY=0.711184
PRIMER_RIGHT_27_PENALTY=1.379351
PRIMER_LEFT_27_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_27_SEQUENCE=CGGTGAAGTGAGTGGTGTTT
PRIMER_LEFT_27=924,20
PRIMER_RIGHT_27=1752,20
PRIMER_LEFT_27_TM=60.711
PRIMER_RIGHT_27_TM=58.621
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=829
PRIMER_PAIR_28_PENALTY=2.126808
PRIMER_LEFT_28_PENALTY=1.536905
PRIMER_RIGHT_28_PENALTY=0.589903
PRIMER_LEFT_28_SEQUENCE=GAATGTTGGAGTCGTAGGTGG
PRIMER_RIGHT_28_SEQUENCE=ATGGAACAATGGAACTGGCT
PRIMER_LEFT_28=921,21
PRIMER_RIGHT_28=1722,20
PRIMER_LEFT_28_TM=59.463
PRIMER_RIGHT_28_TM=59.410
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=802
PRIMER_PAIR_29_PENALTY=2.178328
PRIMER_LEFT_29_PENALTY=0.711184
PRIMER_RIGHT_29_PENALTY=1.467144
PRIMER_LEFT_29_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_29_SEQUENCE=ACGGTGAAGTGAGTGGTGTTT
PRIMER_LEFT_29=924,20
PRIMER_RIGHT_29=1753,21
PRIMER_LEFT_29_TM=60.711
PRIMER_RIGHT_29_TM=59.533
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=830
PRIMER_PAIR_30_PENALTY=2.178328
PRIMER_LEFT_30_PENALTY=0.711184
PRIMER_RIGHT_30_PENALTY=1.467144
PRIMER_LEFT_30_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_30_SEQUENCE=AACGGTGAAGTGAGTGGTGTT
PRIMER_LEFT_30=924,20
PRIMER_RIGHT_30=1754,21
PRIMER_LEFT_30_TM=60.711
PRIMER_RIGHT_30_TM=59.533
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=831
PRIMER_PAIR_31_PENALTY=2.233169
PRIMER_LEFT_31_PENALTY=0.272667
PRIMER_RIGHT_31_PENALTY=1.960502
PRIMER_LEFT_31_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_31_SEQUENCE=ACGGTGAAGTGAGTGGTGTT
PRIMER_LEFT_31=925,20
PRIMER_RIGHT_31=1753,20
PRIMER_LEFT_31_TM=59.727
PRIMER_RIGHT_31_TM=58.039
PRIMER_LEFT_31_GC_PERCENT=60.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=829
PRIMER_PAIR_32_PENALTY=2.233169
PRIMER_LEFT_32_PENALTY=0.272667
PRIMER_RIGHT_32_PENALTY=1.960502
PRIMER_LEFT_32_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_32_SEQUENCE=AACGGTGAAGTGAGTGGTGT
PRIMER_LEFT_32=925,20
PRIMER_RIGHT_32=1754,20
PRIMER_LEFT_32_TM=59.727
PRIMER_RIGHT_32_TM=58.039
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=830
PRIMER_PAIR_33_PENALTY=2.267952
PRIMER_LEFT_33_PENALTY=0.711184
PRIMER_RIGHT_33_PENALTY=1.556768
PRIMER_LEFT_33_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_33_SEQUENCE=TATGGAACAATGGAACTGGCT
PRIMER_LEFT_33=924,20
PRIMER_RIGHT_33=1723,21
PRIMER_LEFT_33_TM=60.711
PRIMER_RIGHT_33_TM=59.443
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=800
PRIMER_PAIR_34_PENALTY=2.290758
PRIMER_LEFT_34_PENALTY=1.536905
PRIMER_RIGHT_34_PENALTY=0.753853
PRIMER_LEFT_34_SEQUENCE=GAATGTTGGAGTCGTAGGTGG
PRIMER_RIGHT_34_SEQUENCE=AACTGGCTGTGGTGCTTGTT
PRIMER_LEFT_34=921,21
PRIMER_RIGHT_34=1710,20
PRIMER_LEFT_34_TM=59.463
PRIMER_RIGHT_34_TM=60.754
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=790
PRIMER_PAIR_35_PENALTY=2.302943
PRIMER_LEFT_35_PENALTY=0.272667
PRIMER_RIGHT_35_PENALTY=2.030276
PRIMER_LEFT_35_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_35_SEQUENCE=TCAATAACGGTGAAGTGAGTGG
PRIMER_LEFT_35=925,20
PRIMER_RIGHT_35=1759,22
PRIMER_LEFT_35_TM=59.727
PRIMER_RIGHT_35_TM=60.030
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=835
PRIMER_PAIR_36_PENALTY=2.306172
PRIMER_LEFT_36_PENALTY=0.711184
PRIMER_RIGHT_36_PENALTY=1.594988
PRIMER_LEFT_36_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_36_SEQUENCE=CGGTGAAGTGAGTGGTGTTTC
PRIMER_LEFT_36=924,20
PRIMER_RIGHT_36=1752,21
PRIMER_LEFT_36_TM=60.711
PRIMER_RIGHT_36_TM=60.595
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=2.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=829
PRIMER_PAIR_37_PENALTY=2.310044
PRIMER_LEFT_37_PENALTY=0.272667
PRIMER_RIGHT_37_PENALTY=2.037376
PRIMER_LEFT_37_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_37_SEQUENCE=TGGAACAATGGAACTGGCTG
PRIMER_LEFT_37=925,20
PRIMER_RIGHT_37=1721,20
PRIMER_LEFT_37_TM=59.727
PRIMER_RIGHT_37_TM=62.037
PRIMER_LEFT_37_GC_PERCENT=60.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=797
PRIMER_PAIR_38_PENALTY=2.327567
PRIMER_LEFT_38_PENALTY=0.272667
PRIMER_RIGHT_38_PENALTY=2.054900
PRIMER_LEFT_38_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_38_SEQUENCE=ATTGTGGGTATGGAGAATTTGG
PRIMER_LEFT_38=925,20
PRIMER_RIGHT_38=1789,22
PRIMER_LEFT_38_TM=59.727
PRIMER_RIGHT_38_TM=59.945
PRIMER_LEFT_38_GC_PERCENT=60.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=8.8000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=865
PRIMER_PAIR_39_PENALTY=2.365693
PRIMER_LEFT_39_PENALTY=0.272667
PRIMER_RIGHT_39_PENALTY=2.093026
PRIMER_LEFT_39_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_39_SEQUENCE=AATCAGCTCTACTCCTTCGGTG
PRIMER_LEFT_39=925,20
PRIMER_RIGHT_39=1419,22
PRIMER_LEFT_39_TM=59.727
PRIMER_RIGHT_39_TM=59.907
PRIMER_LEFT_39_GC_PERCENT=60.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=9.9000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=495
PRIMER_PAIR_40_PENALTY=2.377917
PRIMER_LEFT_40_PENALTY=1.946991
PRIMER_RIGHT_40_PENALTY=0.430926
PRIMER_LEFT_40_SEQUENCE=ATGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_40_SEQUENCE=ACAATGGAACTGGCTGTGGT
PRIMER_LEFT_40=923,21
PRIMER_RIGHT_40=1717,20
PRIMER_LEFT_40_TM=60.947
PRIMER_RIGHT_40_TM=60.431
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=795
PRIMER_PAIR_41_PENALTY=2.382716
PRIMER_LEFT_41_PENALTY=0.272667
PRIMER_RIGHT_41_PENALTY=2.110049
PRIMER_LEFT_41_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_41_SEQUENCE=TGGGTATGGAGAATTTGGAAG
PRIMER_LEFT_41=925,20
PRIMER_RIGHT_41=1785,21
PRIMER_LEFT_41_TM=59.727
PRIMER_RIGHT_41_TM=58.890
PRIMER_LEFT_41_GC_PERCENT=60.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=861
PRIMER_PAIR_42_PENALTY=2.398069
PRIMER_LEFT_42_PENALTY=1.536905
PRIMER_RIGHT_42_PENALTY=0.861164
PRIMER_LEFT_42_SEQUENCE=GAATGTTGGAGTCGTAGGTGG
PRIMER_RIGHT_42_SEQUENCE=GAACAATGGAACTGGCTGTG
PRIMER_LEFT_42=921,21
PRIMER_RIGHT_42=1719,20
PRIMER_LEFT_42_TM=59.463
PRIMER_RIGHT_42_TM=59.139
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=799
PRIMER_PAIR_43_PENALTY=2.440956
PRIMER_LEFT_43_PENALTY=0.711184
PRIMER_RIGHT_43_PENALTY=1.729772
PRIMER_LEFT_43_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_43_SEQUENCE=TGTGGGTATGGAGAATTTGG
PRIMER_LEFT_43=924,20
PRIMER_RIGHT_43=1787,20
PRIMER_LEFT_43_TM=60.711
PRIMER_RIGHT_43_TM=58.270
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=8.8000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=864
PRIMER_PAIR_44_PENALTY=2.491303
PRIMER_LEFT_44_PENALTY=1.536905
PRIMER_RIGHT_44_PENALTY=0.954397
PRIMER_LEFT_44_SEQUENCE=GAATGTTGGAGTCGTAGGTGG
PRIMER_RIGHT_44_SEQUENCE=AACAATGGAACTGGCTGTGG
PRIMER_LEFT_44=921,21
PRIMER_RIGHT_44=1718,20
PRIMER_LEFT_44_TM=59.463
PRIMER_RIGHT_44_TM=60.954
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=798
PRIMER_PAIR_45_PENALTY=2.510027
PRIMER_LEFT_45_PENALTY=0.711184
PRIMER_RIGHT_45_PENALTY=1.798843
PRIMER_LEFT_45_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_45_SEQUENCE=GGACATTGTGGGTATGGAGA
PRIMER_LEFT_45=924,20
PRIMER_RIGHT_45=1793,20
PRIMER_LEFT_45_TM=60.711
PRIMER_RIGHT_45_TM=58.201
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.7000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=870
PRIMER_PAIR_46_PENALTY=2.524358
PRIMER_LEFT_46_PENALTY=0.711184
PRIMER_RIGHT_46_PENALTY=1.813174
PRIMER_LEFT_46_SEQUENCE=TGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_46_SEQUENCE=TCAGCTCTACTCCTTCGGTG
PRIMER_LEFT_46=924,20
PRIMER_RIGHT_46=1417,20
PRIMER_LEFT_46_TM=60.711
PRIMER_RIGHT_46_TM=58.187
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=494
PRIMER_PAIR_47_PENALTY=2.536894
PRIMER_LEFT_47_PENALTY=1.946991
PRIMER_RIGHT_47_PENALTY=0.589903
PRIMER_LEFT_47_SEQUENCE=ATGTTGGAGTCGTAGGTGGCT
PRIMER_RIGHT_47_SEQUENCE=ATGGAACAATGGAACTGGCT
PRIMER_LEFT_47=923,21
PRIMER_RIGHT_47=1722,20
PRIMER_LEFT_47_TM=60.947
PRIMER_RIGHT_47_TM=59.410
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=800
PRIMER_PAIR_48_PENALTY=2.568844
PRIMER_LEFT_48_PENALTY=0.272667
PRIMER_RIGHT_48_PENALTY=2.296177
PRIMER_LEFT_48_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_48_SEQUENCE=GTGGGTATGGAGAATTTGGAAG
PRIMER_LEFT_48=925,20
PRIMER_RIGHT_48=1786,22
PRIMER_LEFT_48_TM=59.727
PRIMER_RIGHT_48_TM=59.704
PRIMER_LEFT_48_GC_PERCENT=60.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=862
PRIMER_PAIR_49_PENALTY=2.645224
PRIMER_LEFT_49_PENALTY=0.272667
PRIMER_RIGHT_49_PENALTY=2.372557
PRIMER_LEFT_49_SEQUENCE=GTTGGAGTCGTAGGTGGCTC
PRIMER_RIGHT_49_SEQUENCE=CTATGGAACAATGGAACTGGCT
PRIMER_LEFT_49=925,20
PRIMER_RIGHT_49=1724,22
PRIMER_LEFT_49_TM=59.727
PRIMER_RIGHT_49_TM=60.373
PRIMER_LEFT_49_GC_PERCENT=60.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=800
=
SEQUENCE_ID=MAGIC.426_3_10472514_10473098_sf-2_oy-0_290957_7937_TRUE
SEQUENCE_TARGET=950,684
SEQUENCE_TEMPLATE=GAGCNTCTTNATATNTTATATTTGNTTTTGAAGAATTTGCTATCATCTGTTNTCAANTGTGATNNNNNNNNCTGGAGTATAAGTTACACNTGGTGGAAGAATATANAAGCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAATGCTCGAAGGCATTTCTCTGTNNNNNNNNNNNNNNNNNNNNNNTGAAANAACTCGGTGAATACATGAAACNGTACTGTACTNTCTGCNGAAGATTNNGAGCAACNATATGAGGTAAACGAAAGTTTTGTNTTCTATTNANNNATTGTGTATCATTGANAAAANAATCATATCAGTAATATGCTTNNAAAGAAGAAAGGTAAAAGAATGTTTATATCCACAAATCATNTCATCTTAACTGTCTAATTATNGCCATTANAAANTCNTTCCACGCATCNATTGGCCCGGGCAAACACCAATGNACGCAATCNTTNTAACCTTTCATCCATTTATTTCCCCAGTAACCATTNGGATGCCCATCNGGTCTCATCTGCATAACNCTNGTCACNTCCAANACCGCAAANTTCTTTGCNTTGTTGNCTNGTTTTGTTATACCTTCNAGCTGTTCAATCTGAGATTTCCTGATCTTCATCTCATTGCTCTGNAGGTCAATTTTGTTTTCTCCGAAAGGACTCGTCCTGCTGCAAGTTCCNCCGGTGTCCCAAGTNCCATTNTCGAAATGCGCAGGTGAAATAGTCCTCAGTACCGTCACTAAATCTTNCTTACACNTCTCACACTCNTTGATTTGCNTNANGACAGCNGAGTAAACAAGCTTAAACCCTTNTTCCGGGCTAATCTGAGTCATGTTTGGTAAGTTACAGTAAATGCAACCNAGTGTNTCATCTCCTCTATGTATANATATNGGTCTGAANAACCAATGAGCAGCCGAGACAATAGCAATGTNTGTATTCGGTAGACCATTAAACCACCCTTCNTCGATCTTGCNNATATCTAGATCAAACAATCCAGTACCTGTATTGTTACTATCTCTNCTCTCTCGTTCCTCNACAAGAAACTTAGTCCACNAGGTCGAGAGNGTAAAATCGTGCTTCGGAAAGTACCAAATCCTGTTTCTATCTTCTCCATCCTTGTAGATGTNCTTAGGAGTTTCTTCCTGNACAAGCAAACNCNNNAAAAAAGCTTAAGCAAAAGCAANTATTNNCTTCTTGCAAANAAAGCAANNTATNTNCATACCATTGACAACAAGCAAAGAAGAGATTCCATATGGTTCCTAGCNACAGAATCACCNATNAANTTCATCTTCTTCCCTCGAACCATACTGAGAAACGCCTTTGGATTAAACCTCGGAAGATCGCANCCATCGGGTTTCCATCTCCAGAACAAGAAATCTNTGTCGGGTCTCCCTTGTTTTATGCAGTTCTTCGAGTCGGGAATCGTAGNACAGCTCGAGTTTGTGTATAAAGATCCTCTTTTGTCTGGAACCCAATGTCCCTTGAACAAATCACACTCAGGATCTAACCNAAAAANCNCAANACAAAAGACAAACAAATCANTANANTTTCAAACACAAACTAAAGAACACANAATAGGAATTAAACAAAACTGANANATTNNNNNNAAAAAANACAGCAAGNTTGTGGGAATANTTAAATATNATAATCAAAAGTTTTNTAAGTTNACAAATACAAATANTCCCACGAAATTTTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATGTTNNNNNNNNCTCATATTTTACAAAATNTCAAGAACAAAAAACAGCAAAAACGTACGTGGGAANAAATAAANNNNNNNNCATAATTTTNAAANACTTNANAGNATACAAATAATCAAAATAAATCCNAAGAACAAGTTCGTGGANANAAAAAAAGAAAGTNACAAAANCANAANANTTTGATGATCATNATNTNTNTTTTCTATGGTTTTACCTTACCGATTTGAGGTTGGAANGAAAGAACATTTTGATCNGCNATGGATTTAAAAGGATTCTGATAAACAAAGAAACCGGTGAAGAAAAGAGTTATACANAAGGAAGACAAGAAAAATGGAGAAAATCTTCCAATGTTCATCATCATCCATCTCTCTGATTTTTTCTCTGAAGTTTCACGAAAAANCGATGACGATGATTTCATCGTTGCATGTCGATCATCCCTAGAAACATTACCAACGAATNAACTTATATATGTCTATAATTCTATATATACACGCGTNGAGATTTTTCTTTTNATTCTCTTTCCTTANAAAAGTTTAAATTTGGGTNGTGAAAATGAAAANTTGTGAAGGANAAAANGTTATAACGGCGATTTTTNATGATATTTTTGTTTNTTTTNTTCAATNATGANGTAACGGTAGCCGTCAGATGAATACACGTGGCNTGCTACGAGGTGGTNTGTGTTTGCATGTGAATTGTGGATTGGTAAGNTTTTTCTAGTTTTCTTGCACTNAACCANATTTCACGGCGTAATCTNTTTTNTTTCCTTTGTTGAAATTNTGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_10908069_10908075_wu-0_bur-0_135331_164_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=GATGCTTTTNGAATTCGCNGTATTGCTATTGCTTCTGTTGCTTTTGTTAGACTCTGTTTTGGAGAAGAAAGANTTGTTTCCACTACTCTTGTGAGAGAGACTCTTAGAGTTAGAGGAAGAGAAGCCACGATGATGATTTTGATTATGATGNGCATTTCTTGATTTATGCAATAGCATGTCGAAATCGAGCGATGGTGTGCAAGCACGTCCTTCGCCGCAAGATTTGCTTCTTCGAAACGCAGTGGCNGCATCTTGAACGAGCATTAGGTCAAGAACTTGCTCAGTNGTTCTGTCCTTCCAGTTGTGAATAGAGCTATTTCTAGGTGACGTNGCTGCTGAGTTTGGGAGGCTGCAGCTCACAAACTTCGTCTTCATTTGATGATCNNNNNNNNNGTCATCGATGTCTCTCGGACGTGAGACTATCATATCGAAGCTCCCATTCCTNGACAATAGCGATTGCGGATCAACCGAGATCCGCCTCTCGGATACGGATTGCATACTCGTCTTCTTGATAAGAATCTTGGGCTCGTCTCCACCACCTAGAACCTCAAAGTCATGCCAGTCCTCGAGACTACTATCGTCCCTGAAGCTGAGAACATCTGTNNCCATTTGTTNCTCTTTGGCCTGNAANNGAATGAGGACTAATAAGAAAATGAAAATATTGGCAAAANGNCTAANACAGATTGATTGATACTTAAATACGGAGCAGGATGGGTTGCACGTCGGTGNCTACNTTAGTCATGACTTGCACGTTTAATATAATATAACCCAAAGTGTTGTTGACTTCATCTNCTAAAAAANGAAAAATGNNTCATNNTTTTTTTATTNGTTAANTATCNCANTATCGTTGACATGAGACCATNCCCANGATCAAGACAAGATCAAAAAGATAGATCTGCCTGCACGAACTACCTAATTATAATATTACCAAAGNATTTGGAGGGTNGTAATCTGCATCAGTGAAGACCATAAGTAGAAGCCATAAATCTTGAATACNTCTNCNNNTNNATGNACNTNGTNCTNTATNNNNTACNTNNTACTTGGNNANTTNTTTNTNNTTGGTNACNTNTNNTTNTCNTTGNTNACTNTNCNCGNNANACTTNGNTATANNNCTTNNTNATANNNCTTNTNNNTNNNTCTNNTATTTTTNNNTTNNNNTNNNTTTTNCNNNNNNNNNNNNNNNNNCTTTGAATTTTNNTGTGTCTTTTNCTACAAATATTTCTTCACTTTCATTGAAATTCATCNCCTTCAATGGATGCACNTACGTCCGTTAGTCATNNNTGGCTTTAGGCCTCATATNTATTCTAGNTGCTCTAGTATATCCCTCNAAAATGGACGGTGAGAAACAATACCAATTTTTNATCCCAAGGGATTTGAACCGGTTATGTTTGAATAACATAGNGATTTGAACGTCAAATCAACAANTTACGAAACGATTAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGCTAAAACATTTTTGAANGTTTTCAAATATGGATTTGATCGACAAANATAGAAGTCATATACTCCTTGATTTTTCCTTGGGGATTGGTGNATCCTTCATTTNCATTGTAGGNGACGNTCATCATNCGTGNAAGATCAATGGTTGCCTTAAGAAGAAAATGTGGCACGCCAGTTGTTGNCAAGAACTCCTCATTTAGTGTCTTATTGGCANCTNCAACNATTTTANNAAGCTCCCTAAAAGNTTCCTCTTTGGTAACCCCATATTGCTTCATATANCAGTTNACCGCGTTCGNGANATATCCTCTACTCATGTCNTCCTACTCAATATTTGNGTAAGTNGGTAAGATTTACAAGAAAGCAGTTATACGCGAGATGTAAAGAAANNATTTATGTATTTGTACCTCATAACCCATNAAATCATTNATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.350503
PRIMER_LEFT_0_PENALTY=0.083883
PRIMER_RIGHT_0_PENALTY=0.266620
PRIMER_LEFT_0_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_0_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_0=695,20
PRIMER_RIGHT_0=1666,20
PRIMER_LEFT_0_TM=59.916
PRIMER_RIGHT_0_TM=59.733
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=972
PRIMER_PAIR_1_PENALTY=0.541950
PRIMER_LEFT_1_PENALTY=0.285121
PRIMER_RIGHT_1_PENALTY=0.256829
PRIMER_LEFT_1_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_1_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_1=893,20
PRIMER_RIGHT_1=1748,20
PRIMER_LEFT_1_TM=60.285
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=0.551741
PRIMER_LEFT_2_PENALTY=0.285121
PRIMER_RIGHT_2_PENALTY=0.266620
PRIMER_LEFT_2_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_2_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_2=893,20
PRIMER_RIGHT_2=1666,20
PRIMER_LEFT_2_TM=60.285
PRIMER_RIGHT_2_TM=59.733
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=774
PRIMER_PAIR_3_PENALTY=0.566115
PRIMER_LEFT_3_PENALTY=0.083883
PRIMER_RIGHT_3_PENALTY=0.482233
PRIMER_LEFT_3_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_3_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_3=695,20
PRIMER_RIGHT_3=1667,20
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=60.482
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=973
PRIMER_PAIR_4_PENALTY=0.767353
PRIMER_LEFT_4_PENALTY=0.285121
PRIMER_RIGHT_4_PENALTY=0.482233
PRIMER_LEFT_4_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_4_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_4=893,20
PRIMER_RIGHT_4=1667,20
PRIMER_LEFT_4_TM=60.285
PRIMER_RIGHT_4_TM=60.482
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=775
PRIMER_PAIR_5_PENALTY=0.769116
PRIMER_LEFT_5_PENALTY=0.512286
PRIMER_RIGHT_5_PENALTY=0.256829
PRIMER_LEFT_5_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_5_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_5=896,20
PRIMER_RIGHT_5=1748,20
PRIMER_LEFT_5_TM=59.488
PRIMER_RIGHT_5_TM=60.257
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.5000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=853
PRIMER_PAIR_6_PENALTY=0.778907
PRIMER_LEFT_6_PENALTY=0.512286
PRIMER_RIGHT_6_PENALTY=0.266620
PRIMER_LEFT_6_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_6_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_6=896,20
PRIMER_RIGHT_6=1666,20
PRIMER_LEFT_6_TM=59.488
PRIMER_RIGHT_6_TM=59.733
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=771
PRIMER_PAIR_7_PENALTY=0.979374
PRIMER_LEFT_7_PENALTY=0.712754
PRIMER_RIGHT_7_PENALTY=0.266620
PRIMER_LEFT_7_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_7_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_7=697,20
PRIMER_RIGHT_7=1666,20
PRIMER_LEFT_7_TM=60.713
PRIMER_RIGHT_7_TM=59.733
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=970
PRIMER_PAIR_8_PENALTY=0.979374
PRIMER_LEFT_8_PENALTY=0.712754
PRIMER_RIGHT_8_PENALTY=0.266620
PRIMER_LEFT_8_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_8_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_8=696,20
PRIMER_RIGHT_8=1666,20
PRIMER_LEFT_8_TM=60.713
PRIMER_RIGHT_8_TM=59.733
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=0.994519
PRIMER_LEFT_9_PENALTY=0.512286
PRIMER_RIGHT_9_PENALTY=0.482233
PRIMER_LEFT_9_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_9_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_9=896,20
PRIMER_RIGHT_9=1667,20
PRIMER_LEFT_9_TM=59.488
PRIMER_RIGHT_9_TM=60.482
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.5000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=772
PRIMER_PAIR_10_PENALTY=1.194986
PRIMER_LEFT_10_PENALTY=0.712754
PRIMER_RIGHT_10_PENALTY=0.482233
PRIMER_LEFT_10_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_10_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_10=697,20
PRIMER_RIGHT_10=1667,20
PRIMER_LEFT_10_TM=60.713
PRIMER_RIGHT_10_TM=60.482
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=971
PRIMER_PAIR_11_PENALTY=1.194986
PRIMER_LEFT_11_PENALTY=0.712754
PRIMER_RIGHT_11_PENALTY=0.482233
PRIMER_LEFT_11_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_11_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_11=696,20
PRIMER_RIGHT_11=1667,20
PRIMER_LEFT_11_TM=60.713
PRIMER_RIGHT_11_TM=60.482
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=1.227016
PRIMER_LEFT_12_PENALTY=0.083883
PRIMER_RIGHT_12_PENALTY=1.143133
PRIMER_LEFT_12_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_12_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_12=695,20
PRIMER_RIGHT_12=1662,20
PRIMER_LEFT_12_TM=59.916
PRIMER_RIGHT_12_TM=61.143
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=968
PRIMER_PAIR_13_PENALTY=1.227016
PRIMER_LEFT_13_PENALTY=0.083883
PRIMER_RIGHT_13_PENALTY=1.143133
PRIMER_LEFT_13_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_13_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_13=695,20
PRIMER_RIGHT_13=1663,20
PRIMER_LEFT_13_TM=59.916
PRIMER_RIGHT_13_TM=61.143
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=969
PRIMER_PAIR_14_PENALTY=1.239333
PRIMER_LEFT_14_PENALTY=0.083883
PRIMER_RIGHT_14_PENALTY=1.155450
PRIMER_LEFT_14_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_14_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_14=695,20
PRIMER_RIGHT_14=1352,21
PRIMER_LEFT_14_TM=59.916
PRIMER_RIGHT_14_TM=59.845
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=658
PRIMER_PAIR_15_PENALTY=1.239333
PRIMER_LEFT_15_PENALTY=0.083883
PRIMER_RIGHT_15_PENALTY=1.155450
PRIMER_LEFT_15_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_15_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_15=695,20
PRIMER_RIGHT_15=1353,21
PRIMER_LEFT_15_TM=59.916
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=659
PRIMER_PAIR_16_PENALTY=1.252490
PRIMER_LEFT_16_PENALTY=0.995660
PRIMER_RIGHT_16_PENALTY=0.256829
PRIMER_LEFT_16_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_16_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_16=894,20
PRIMER_RIGHT_16=1748,20
PRIMER_LEFT_16_TM=60.996
PRIMER_RIGHT_16_TM=60.257
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=855
PRIMER_PAIR_17_PENALTY=1.262281
PRIMER_LEFT_17_PENALTY=0.995660
PRIMER_RIGHT_17_PENALTY=0.266620
PRIMER_LEFT_17_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_17_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_17=894,20
PRIMER_RIGHT_17=1666,20
PRIMER_LEFT_17_TM=60.996
PRIMER_RIGHT_17_TM=59.733
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=773
PRIMER_PAIR_18_PENALTY=1.428253
PRIMER_LEFT_18_PENALTY=0.285121
PRIMER_RIGHT_18_PENALTY=1.143133
PRIMER_LEFT_18_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_18_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_18=893,20
PRIMER_RIGHT_18=1662,20
PRIMER_LEFT_18_TM=60.285
PRIMER_RIGHT_18_TM=61.143
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=770
PRIMER_PAIR_19_PENALTY=1.428253
PRIMER_LEFT_19_PENALTY=0.285121
PRIMER_RIGHT_19_PENALTY=1.143133
PRIMER_LEFT_19_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_19_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_19=893,20
PRIMER_RIGHT_19=1663,20
PRIMER_LEFT_19_TM=60.285
PRIMER_RIGHT_19_TM=61.143
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=771
PRIMER_PAIR_20_PENALTY=1.440571
PRIMER_LEFT_20_PENALTY=0.285121
PRIMER_RIGHT_20_PENALTY=1.155450
PRIMER_LEFT_20_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_20_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_20=893,20
PRIMER_RIGHT_20=1352,21
PRIMER_LEFT_20_TM=60.285
PRIMER_RIGHT_20_TM=59.845
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=460
PRIMER_PAIR_21_PENALTY=1.440571
PRIMER_LEFT_21_PENALTY=0.285121
PRIMER_RIGHT_21_PENALTY=1.155450
PRIMER_LEFT_21_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_21_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_21=893,20
PRIMER_RIGHT_21=1353,21
PRIMER_LEFT_21_TM=60.285
PRIMER_RIGHT_21_TM=59.845
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=461
PRIMER_PAIR_22_PENALTY=1.465064
PRIMER_LEFT_22_PENALTY=0.083883
PRIMER_RIGHT_22_PENALTY=1.381181
PRIMER_LEFT_22_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_22_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_22=695,20
PRIMER_RIGHT_22=1661,20
PRIMER_LEFT_22_TM=59.916
PRIMER_RIGHT_22_TM=58.619
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=967
PRIMER_PAIR_23_PENALTY=1.477893
PRIMER_LEFT_23_PENALTY=0.995660
PRIMER_RIGHT_23_PENALTY=0.482233
PRIMER_LEFT_23_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_23_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_23=894,20
PRIMER_RIGHT_23=1667,20
PRIMER_LEFT_23_TM=60.996
PRIMER_RIGHT_23_TM=60.482
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=774
PRIMER_PAIR_24_PENALTY=1.581829
PRIMER_LEFT_24_PENALTY=0.426379
PRIMER_RIGHT_24_PENALTY=1.155450
PRIMER_LEFT_24_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_24_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_24=527,20
PRIMER_RIGHT_24=1352,21
PRIMER_LEFT_24_TM=59.574
PRIMER_RIGHT_24_TM=59.845
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=826
PRIMER_PAIR_25_PENALTY=1.581829
PRIMER_LEFT_25_PENALTY=0.426379
PRIMER_RIGHT_25_PENALTY=1.155450
PRIMER_LEFT_25_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_25_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_25=527,20
PRIMER_RIGHT_25=1353,21
PRIMER_LEFT_25_TM=59.574
PRIMER_RIGHT_25_TM=59.845
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=827
PRIMER_PAIR_26_PENALTY=1.637322
PRIMER_LEFT_26_PENALTY=0.083883
PRIMER_RIGHT_26_PENALTY=1.553439
PRIMER_LEFT_26_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_26_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_26=695,20
PRIMER_RIGHT_26=1661,21
PRIMER_LEFT_26_TM=59.916
PRIMER_RIGHT_26_TM=59.447
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=967
PRIMER_PAIR_27_PENALTY=1.655419
PRIMER_LEFT_27_PENALTY=0.512286
PRIMER_RIGHT_27_PENALTY=1.143133
PRIMER_LEFT_27_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_27_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_27=896,20
PRIMER_RIGHT_27=1662,20
PRIMER_LEFT_27_TM=59.488
PRIMER_RIGHT_27_TM=61.143
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.5000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=767
PRIMER_PAIR_28_PENALTY=1.655419
PRIMER_LEFT_28_PENALTY=0.512286
PRIMER_RIGHT_28_PENALTY=1.143133
PRIMER_LEFT_28_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_28_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_28=896,20
PRIMER_RIGHT_28=1663,20
PRIMER_LEFT_28_TM=59.488
PRIMER_RIGHT_28_TM=61.143
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.5000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=768
PRIMER_PAIR_29_PENALTY=1.666302
PRIMER_LEFT_29_PENALTY=0.285121
PRIMER_RIGHT_29_PENALTY=1.381181
PRIMER_LEFT_29_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_29_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_29=893,20
PRIMER_RIGHT_29=1661,20
PRIMER_LEFT_29_TM=60.285
PRIMER_RIGHT_29_TM=58.619
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=769
PRIMER_PAIR_30_PENALTY=1.667737
PRIMER_LEFT_30_PENALTY=0.512286
PRIMER_RIGHT_30_PENALTY=1.155450
PRIMER_LEFT_30_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_30_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_30=896,20
PRIMER_RIGHT_30=1352,21
PRIMER_LEFT_30_TM=59.488
PRIMER_RIGHT_30_TM=59.845
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.5000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=457
PRIMER_PAIR_31_PENALTY=1.667737
PRIMER_LEFT_31_PENALTY=0.512286
PRIMER_RIGHT_31_PENALTY=1.155450
PRIMER_LEFT_31_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_31_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_31=896,20
PRIMER_RIGHT_31=1353,21
PRIMER_LEFT_31_TM=59.488
PRIMER_RIGHT_31_TM=59.845
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=458
PRIMER_PAIR_32_PENALTY=1.669226
PRIMER_LEFT_32_PENALTY=0.513776
PRIMER_RIGHT_32_PENALTY=1.155450
PRIMER_LEFT_32_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_32_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_32=423,20
PRIMER_RIGHT_32=1352,21
PRIMER_LEFT_32_TM=59.486
PRIMER_RIGHT_32_TM=59.845
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=930
PRIMER_PAIR_33_PENALTY=1.669226
PRIMER_LEFT_33_PENALTY=0.513776
PRIMER_RIGHT_33_PENALTY=1.155450
PRIMER_LEFT_33_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_33_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_33=423,20
PRIMER_RIGHT_33=1353,21
PRIMER_LEFT_33_TM=59.486
PRIMER_RIGHT_33_TM=59.845
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=1.703379
PRIMER_LEFT_34_PENALTY=1.446550
PRIMER_RIGHT_34_PENALTY=0.256829
PRIMER_LEFT_34_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_34_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_34=895,21
PRIMER_RIGHT_34=1748,20
PRIMER_LEFT_34_TM=60.447
PRIMER_RIGHT_34_TM=60.257
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=854
PRIMER_PAIR_35_PENALTY=1.713170
PRIMER_LEFT_35_PENALTY=1.446550
PRIMER_RIGHT_35_PENALTY=0.266620
PRIMER_LEFT_35_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_35_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_35=895,21
PRIMER_RIGHT_35=1666,20
PRIMER_LEFT_35_TM=60.447
PRIMER_RIGHT_35_TM=59.733
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.5000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=772
PRIMER_PAIR_36_PENALTY=1.740964
PRIMER_LEFT_36_PENALTY=1.484135
PRIMER_RIGHT_36_PENALTY=0.256829
PRIMER_LEFT_36_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_36_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_36=891,21
PRIMER_RIGHT_36=1748,20
PRIMER_LEFT_36_TM=59.516
PRIMER_RIGHT_36_TM=60.257
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.1000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=858
PRIMER_PAIR_37_PENALTY=1.750755
PRIMER_LEFT_37_PENALTY=1.484135
PRIMER_RIGHT_37_PENALTY=0.266620
PRIMER_LEFT_37_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_37_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_37=891,21
PRIMER_RIGHT_37=1666,20
PRIMER_LEFT_37_TM=59.516
PRIMER_RIGHT_37_TM=59.733
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.1000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=776
PRIMER_PAIR_38_PENALTY=1.785200
PRIMER_LEFT_38_PENALTY=1.528370
PRIMER_RIGHT_38_PENALTY=0.256829
PRIMER_LEFT_38_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_38_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_38=892,20
PRIMER_RIGHT_38=1748,20
PRIMER_LEFT_38_TM=58.472
PRIMER_RIGHT_38_TM=60.257
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.1000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=857
PRIMER_PAIR_39_PENALTY=1.794991
PRIMER_LEFT_39_PENALTY=1.528370
PRIMER_RIGHT_39_PENALTY=0.266620
PRIMER_LEFT_39_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_39_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_39=892,20
PRIMER_RIGHT_39=1666,20
PRIMER_LEFT_39_TM=58.472
PRIMER_RIGHT_39_TM=59.733
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.1000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=775
PRIMER_PAIR_40_PENALTY=1.811843
PRIMER_LEFT_40_PENALTY=0.656393
PRIMER_RIGHT_40_PENALTY=1.155450
PRIMER_LEFT_40_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_40_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_40=545,20
PRIMER_RIGHT_40=1352,21
PRIMER_LEFT_40_TM=60.656
PRIMER_RIGHT_40_TM=59.845
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=808
PRIMER_PAIR_41_PENALTY=1.811843
PRIMER_LEFT_41_PENALTY=0.656393
PRIMER_RIGHT_41_PENALTY=1.155450
PRIMER_LEFT_41_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_41_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_41=545,20
PRIMER_RIGHT_41=1353,21
PRIMER_LEFT_41_TM=60.656
PRIMER_RIGHT_41_TM=59.845
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=809
PRIMER_PAIR_42_PENALTY=1.838560
PRIMER_LEFT_42_PENALTY=0.285121
PRIMER_RIGHT_42_PENALTY=1.553439
PRIMER_LEFT_42_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_42_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_42=893,20
PRIMER_RIGHT_42=1661,21
PRIMER_LEFT_42_TM=60.285
PRIMER_RIGHT_42_TM=59.447
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=769
PRIMER_PAIR_43_PENALTY=1.855887
PRIMER_LEFT_43_PENALTY=0.712754
PRIMER_RIGHT_43_PENALTY=1.143133
PRIMER_LEFT_43_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_43_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_43=697,20
PRIMER_RIGHT_43=1662,20
PRIMER_LEFT_43_TM=60.713
PRIMER_RIGHT_43_TM=61.143
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=1.855887
PRIMER_LEFT_44_PENALTY=0.712754
PRIMER_RIGHT_44_PENALTY=1.143133
PRIMER_LEFT_44_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_44_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_44=697,20
PRIMER_RIGHT_44=1663,20
PRIMER_LEFT_44_TM=60.713
PRIMER_RIGHT_44_TM=61.143
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=967
PRIMER_PAIR_45_PENALTY=1.855887
PRIMER_LEFT_45_PENALTY=0.712754
PRIMER_RIGHT_45_PENALTY=1.143133
PRIMER_LEFT_45_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_45_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_45=696,20
PRIMER_RIGHT_45=1662,20
PRIMER_LEFT_45_TM=60.713
PRIMER_RIGHT_45_TM=61.143
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=967
PRIMER_PAIR_46_PENALTY=1.855887
PRIMER_LEFT_46_PENALTY=0.712754
PRIMER_RIGHT_46_PENALTY=1.143133
PRIMER_LEFT_46_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_46_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_46=696,20
PRIMER_RIGHT_46=1663,20
PRIMER_LEFT_46_TM=60.713
PRIMER_RIGHT_46_TM=61.143
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=968
PRIMER_PAIR_47_PENALTY=1.868204
PRIMER_LEFT_47_PENALTY=0.712754
PRIMER_RIGHT_47_PENALTY=1.155450
PRIMER_LEFT_47_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_47_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_47=697,20
PRIMER_RIGHT_47=1352,21
PRIMER_LEFT_47_TM=60.713
PRIMER_RIGHT_47_TM=59.845
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=656
PRIMER_PAIR_48_PENALTY=1.868204
PRIMER_LEFT_48_PENALTY=0.712754
PRIMER_RIGHT_48_PENALTY=1.155450
PRIMER_LEFT_48_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_48_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_48=697,20
PRIMER_RIGHT_48=1353,21
PRIMER_LEFT_48_TM=60.713
PRIMER_RIGHT_48_TM=59.845
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=657
PRIMER_PAIR_49_PENALTY=1.868204
PRIMER_LEFT_49_PENALTY=0.712754
PRIMER_RIGHT_49_PENALTY=1.155450
PRIMER_LEFT_49_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_49_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_49=696,20
PRIMER_RIGHT_49=1352,21
PRIMER_LEFT_49_TM=60.713
PRIMER_RIGHT_49_TM=59.845
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=657
=
SEQUENCE_ID=MAGIC.149_1_12664668_12664718_no-0_kn-0_43042_8797_TRUE
SEQUENCE_TARGET=950,150
SEQUENCE_TEMPLATE=TCGAAGTTGCATTNGTAACTTGGAACAAGTATNNGTTNGAGAACATNTTGTTTTAACCACGNANCTACGTGGTAAGNNGGGNGGTNNNNNNNNNNCNANAGATTTTNTAAATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNGTGAANTNNTTANTAATGTTTATNATGANCCCTATANCTTTTGTTCNAAATCCTNTATTGANCCCTTNANTTTTANTTTTGTATCACTAATTTCATNACATNAAATNNNNNCNNATANTANGTCATTTTATTATGTCAGGTTGGCTTGNGAGGATCGTAATATNNNANTANTNTGCTAAAATNTNCTCTNATNANTTGGCATTNACATGCNGAAGTNNAATATACAAGCNNATNTNTCATGTAAATTCTNCATTNAACGGGTCNAAACTAGAAACCACCTNTTTTNCAATGNTGTTATTNAANGGAAATATGNNAAAAGNNNNNNNNNNNNNNNNNNNNNAGTNGGATTGGGANCAACTNTCANTGCTCATCACAAATCCGANTNTTGAAANGAANGNGTTGNTNCTTCTGCNATACGCACTNNNAATCNCAGTCCACACCATNTNNCNAGAGAGAANTAACANATGNCATAGAGAAGANNCGGTCACTGCNACNACNNTANNGAGAACCNTNGACNAAAACANTNGCAACCAAATNACACTNCTTCGAAAAACNGGGAACCCGAAGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNCATTAAAATGTANNNTTNNNNNNNGTNNTTANATTTTTCANAAACTNAGAAAANATGCNTNTGATGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_4_9954027_9954185_edi-0_mt-0_4142343_3811_TRUE
SEQUENCE_TARGET=950,258
SEQUENCE_TEMPLATE=GCATGTAATAAGCATCTACAAGGAAAAGATCATTCAGAGTAGATGGAAGATAAAAGCAGAGTTGGCTTAAACTAAAACTAAGCAGTGTCATATACCAGAGCAACTCTATAAGAAGATACACTTGCTCTAAACGAGAGAGACTGTCCTCTCTGCAACCCTGAAAAAGATCTAGTTATAAAACCTTAAGTTAGCTAAAGTTAAACCCTTAACTAACANATGACTTACAAGTTATACCTTTTGGCAGTCACAGAAAGAATCAGTACCAACAACGACAACAAGNCTGTCCCATGATAAACCCATTCTGATAGAACCTGTAATATATAAGTTTTGGTGATCATCAGAGAACATTAAAAGTAGGCACTGGTGANTCAAAAAGTTTGAAGAGAGCGTACAGTGAAAAAGAGGAGCATGGGGAAGACAATGAAGATGAAATCCAATGATATGGCAGCCTTCCAATTTCTGGAAATAACAATTTTCTCATCGTCGTTTNTCTTCGATGTAACGGNTTTAACACCATTGTTATCTATAACAAACAAGTNAACATCTTTAGCACTAATCGAACAAAAGTTACATCACTCACTTCAAAAAGCACTCACCAATTTTACAGTGGAAGCCAATGGAGTATCGTAATAGAACCAAGAGCTGAAACAAGAGAAACGTCATTATTATCAGGAATTGAGCTAAACTGAGTCAAAAGAAACTTACAGGGACAATGGTCAATAGTGCTGCGATTTCCAGAATTGAAGATCCATCGAGATTGCTCACAAATCTGTCAAAAGTCAATTTGAACAAAAAAANCTTGAGGAATCACAAATTCTAAAANNCTGACGAANGAGAGNTCATTGAGNGAGTAAACAAAAAGAGAGNNGNNTTTTACTGTTCTTTGAGATGTTTGTTTGGATTTAGAGTCTGCTTTGGTANAGAATCCATCAANACTCNGAGATCAAANAAAGTTGTNAACTTNGAACAGTCGTNCTCGTTGATNAACTATANANATTTANTTTGGTTTGGATCACGAACCGGCCGGTTTANNCNNNATAATCTCAGNTTNCTTTTGTTATTGTTACGAATCTATGCTCGATTTCACATTAAACCAAACATGGACTCACTAATCTGCNTCANACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_19171742_19172025_ws-0_po-0_85675_67951_TRUE
SEQUENCE_TARGET=950,383
SEQUENCE_TEMPLATE=CAGTAGAAGCTGGGAAGCTCATTCTCTCTGTTGACAATACTCTCTCTCGCAAGAAGAAAGTTGCTGCTTACCGTTACACTGTCCGGAAATCTACTACAACCGTCTAATCTATAACCGGTTTTTCAGAAGATTGGTTGCTTTCTTACTACTACTTTTTGTGGGTGTGTTGAGGGGTTTTCTTTCTTGGGTTTGAGTACTAGGTTTGTTTTGCTTACTCAANGGTTCANNNNNNNNNNNANATATCGATNNNNNNNNNAGGAAATCATATGTATAAAAACTATGATTTGTATGACATTTGATGTTTGNCCTTAATATTTTAACTAGAGATTGAAGTTTTAGTCTGCAAAGTGTAAGCTTATGTACTCTCTTGTACCATGTTTAGCTATTGAAGACCAGTGGTCCATTGCTTATGTTTGTTGCATTCTCAGTTATTGTATTCAGTTGTTTTGTATCAGAAGCTGAACTAAGCAATGGAATTTCATACACAATACGACTCTTTAACAGCCAAAATAGGCACCCAAACTTGAAGAATCAATCATTGTTNCATCATATTACCAATCATTGAATAAACTTGAAGATCCAAATTTACTAATTGAATTGTGGGAAGAAAATGTACATCTTCATTTCCCAAATGAGCAGTTTCCATTTTCAGCAGCGAACCTACGTCAAGACCGNTTCGCACGGATGAATAATGACTACAATGATTGTNNNNNNNNNATCTGAACTTATCANAGAACACAGTGACTACGGCCCGACCCAAAATCCCTGTGTATTTTTAATTAGTCGCTGCTACACAGCCCGGCCCAATATAAAGTCTGGCTATGNGTGAGAGGAAAATTTGTTTTATGGTTGACTTTTGAATGTGAGGTTAGGCAGTTCAGTTTTTTAATTTCAATTTGAGTGTGTATTTTATTTTATTTTCAAATAAACTCACAATGTTAATAAATTTTAAATTTTGTTAACTGAAAATCCAAACACCAAACACAAACAGANAAAAAAANCNTTTTTNTTTATATTTTGTGAAATTGTTTTTATGAAACTATGAGAATTTTTGTATCCTTCAAATCTGAAATCTTTTTGAATTCTTTTTATTACTTTACCCTGTGTTATCAAGTTAAATGAGAAATAAAAGGAAATAAGTATAAACCTAAAGAGAACTCATGAGTTGATTTTTTTATTAGNAAAAAAGTACTAGAATCCGATCTTGGTTGTACTCCTAATTAATTGTANTTGATTGAGCTTATAATAGTGTAAAANNNNNNNNNNNAAAAATACGAGATTATNANATNTTTTGAGTCTTAGACCTAAACTCATCTCCTCTTCCTTACATTTCTAAGAAATCCGAGTTAACAAATCAAATCTTAAATAATAAATATTCTTATTCCTAACAATAGCCAATAGGTAATGTTGAAGAACTTGGAGTTTTTGTACAATGTTTTGTTCTGACATGATCACAACTTTTTGGTTAGGACTTGGGAGGTTTCTACGATCGAACCTCTTTAAACATTACATACNTTAACATAAGCAAATCAATTCCAATGANNTTTTTTTTTTTTTAATTTNGAGAAACAACGTGTTATCAGTATGNAATTGGTGGTGAAGAAGTGAAGAGATGTTTCACATCTATGCTTTTGAGGCTTATNTATTTCAGGTTCGNTGGGGATAAAGAACTTTTACAAAAGTGAATAATAGACCAAATAGTCAAATCTAGCTATCGAAGAAAGCNAATTCGTCGGTTTCAAGAAATGGAGGCTACACAAAATTTAATATTATACGTTCCTCTTTCTTTAAATGAAGAAAAGTGTCTATATAGCTGTGGGTTTGACCAATTGGGAACCAATCTTTACTNNTTGATTTTCTTATAATTTTCCAATCTCAGCTAATGATTCCACAATTACGACATAATCGCATCTGATTTTTAAATATTTTCGTTGATCTCATATGTGTTACAACCCAATACACCCTTGCATTCATTAAATTATATCGCTAACATCGAACCCTTTTACTTTATCAAGAAACAAAAAAATNTCGAACCTTTAGCTTCGACTATAAAACTCAATTTGCATTTAAAAACTAAAGTGTAAAATCAAGAATAATACGTGGATTATCAAAAAAGAATAATCGGTGGATGTTAAAAGGAATAAAAGAAAGTACAAAACGTTGTATATTATTGCTCAGTATAACAAAACAATAAATTCTGAGATATTCGTATTCCATCCTTCAAATTAATTATTNTATTCTTTTCCAGANAAANTGTTCCTAAAAAAAAGATTGCATAGGTCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.117668
PRIMER_LEFT_0_PENALTY=0.051345
PRIMER_RIGHT_0_PENALTY=0.066323
PRIMER_LEFT_0_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_0_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_0=624,20
PRIMER_RIGHT_0=1491,20
PRIMER_LEFT_0_TM=60.051
PRIMER_RIGHT_0_TM=60.066
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=868
PRIMER_PAIR_1_PENALTY=0.355127
PRIMER_LEFT_1_PENALTY=0.288804
PRIMER_RIGHT_1_PENALTY=0.066323
PRIMER_LEFT_1_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_1_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_1=511,20
PRIMER_RIGHT_1=1491,20
PRIMER_LEFT_1_TM=59.711
PRIMER_RIGHT_1_TM=60.066
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=981
PRIMER_PAIR_2_PENALTY=0.562113
PRIMER_LEFT_2_PENALTY=0.495790
PRIMER_RIGHT_2_PENALTY=0.066323
PRIMER_LEFT_2_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_2_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_2=676,20
PRIMER_RIGHT_2=1491,20
PRIMER_LEFT_2_TM=59.504
PRIMER_RIGHT_2_TM=60.066
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=816
PRIMER_PAIR_3_PENALTY=0.637445
PRIMER_LEFT_3_PENALTY=0.571122
PRIMER_RIGHT_3_PENALTY=0.066323
PRIMER_LEFT_3_SEQUENCE=CCGGCCCAATATAAAGTCTG
PRIMER_RIGHT_3_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_3=798,20
PRIMER_RIGHT_3=1491,20
PRIMER_LEFT_3_TM=59.429
PRIMER_RIGHT_3_TM=60.066
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=694
PRIMER_PAIR_4_PENALTY=0.882390
PRIMER_LEFT_4_PENALTY=0.288804
PRIMER_RIGHT_4_PENALTY=0.593585
PRIMER_LEFT_4_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_4_SEQUENCE=TCGATCGTAGAAACCTCCCA
PRIMER_LEFT_4=511,20
PRIMER_RIGHT_4=1492,20
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=60.594
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=982
PRIMER_PAIR_5_PENALTY=0.925844
PRIMER_LEFT_5_PENALTY=0.051345
PRIMER_RIGHT_5_PENALTY=0.874500
PRIMER_LEFT_5_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_5_SEQUENCE=TTCGATCGTAGAAACCTCCC
PRIMER_LEFT_5=624,20
PRIMER_RIGHT_5=1493,20
PRIMER_LEFT_5_TM=60.051
PRIMER_RIGHT_5_TM=59.126
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=870
PRIMER_PAIR_6_PENALTY=1.026861
PRIMER_LEFT_6_PENALTY=0.051345
PRIMER_RIGHT_6_PENALTY=0.975516
PRIMER_LEFT_6_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_6_SEQUENCE=CCTCCCAAGTCCTAACCAAA
PRIMER_LEFT_6=624,20
PRIMER_RIGHT_6=1479,20
PRIMER_LEFT_6_TM=60.051
PRIMER_RIGHT_6_TM=59.024
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=856
PRIMER_PAIR_7_PENALTY=1.089375
PRIMER_LEFT_7_PENALTY=0.495790
PRIMER_RIGHT_7_PENALTY=0.593585
PRIMER_LEFT_7_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_7_SEQUENCE=TCGATCGTAGAAACCTCCCA
PRIMER_LEFT_7=676,20
PRIMER_RIGHT_7=1492,20
PRIMER_LEFT_7_TM=59.504
PRIMER_RIGHT_7_TM=60.594
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=817
PRIMER_PAIR_8_PENALTY=1.097176
PRIMER_LEFT_8_PENALTY=0.051345
PRIMER_RIGHT_8_PENALTY=1.045831
PRIMER_LEFT_8_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_8_SEQUENCE=GTTCGATCGTAGAAACCTCCC
PRIMER_LEFT_8=624,20
PRIMER_RIGHT_8=1494,21
PRIMER_LEFT_8_TM=60.051
PRIMER_RIGHT_8_TM=59.954
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=871
PRIMER_PAIR_9_PENALTY=1.097176
PRIMER_LEFT_9_PENALTY=0.051345
PRIMER_RIGHT_9_PENALTY=1.045831
PRIMER_LEFT_9_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_9_SEQUENCE=GGTTCGATCGTAGAAACCTCC
PRIMER_LEFT_9=624,20
PRIMER_RIGHT_9=1495,21
PRIMER_LEFT_9_TM=60.051
PRIMER_RIGHT_9_TM=59.954
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=872
PRIMER_PAIR_10_PENALTY=1.117335
PRIMER_LEFT_10_PENALTY=1.051012
PRIMER_RIGHT_10_PENALTY=0.066323
PRIMER_LEFT_10_SEQUENCE=CCAAAATAGGCACCCAAACT
PRIMER_RIGHT_10_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_10=504,20
PRIMER_RIGHT_10=1491,20
PRIMER_LEFT_10_TM=58.949
PRIMER_RIGHT_10_TM=60.066
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=10.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=988
PRIMER_PAIR_11_PENALTY=1.163304
PRIMER_LEFT_11_PENALTY=0.288804
PRIMER_RIGHT_11_PENALTY=0.874500
PRIMER_LEFT_11_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_11_SEQUENCE=TTCGATCGTAGAAACCTCCC
PRIMER_LEFT_11=511,20
PRIMER_RIGHT_11=1493,20
PRIMER_LEFT_11_TM=59.711
PRIMER_RIGHT_11_TM=59.126
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=983
PRIMER_PAIR_12_PENALTY=1.164708
PRIMER_LEFT_12_PENALTY=0.571122
PRIMER_RIGHT_12_PENALTY=0.593585
PRIMER_LEFT_12_SEQUENCE=CCGGCCCAATATAAAGTCTG
PRIMER_RIGHT_12_SEQUENCE=TCGATCGTAGAAACCTCCCA
PRIMER_LEFT_12=798,20
PRIMER_RIGHT_12=1492,20
PRIMER_LEFT_12_TM=59.429
PRIMER_RIGHT_12_TM=60.594
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=695
PRIMER_PAIR_13_PENALTY=1.169539
PRIMER_LEFT_13_PENALTY=1.103216
PRIMER_RIGHT_13_PENALTY=0.066323
PRIMER_LEFT_13_SEQUENCE=CATTTTCAGCAGCGAACCTAC
PRIMER_RIGHT_13_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_13=643,21
PRIMER_RIGHT_13=1491,20
PRIMER_LEFT_13_TM=59.897
PRIMER_RIGHT_13_TM=60.066
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=10.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=849
PRIMER_PAIR_14_PENALTY=1.201240
PRIMER_LEFT_14_PENALTY=0.051345
PRIMER_RIGHT_14_PENALTY=1.149895
PRIMER_LEFT_14_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_14_SEQUENCE=ACCTCCCAAGTCCTAACCAAA
PRIMER_LEFT_14=624,20
PRIMER_RIGHT_14=1480,21
PRIMER_LEFT_14_TM=60.051
PRIMER_RIGHT_14_TM=59.850
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.8000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=857
PRIMER_PAIR_15_PENALTY=1.201240
PRIMER_LEFT_15_PENALTY=0.051345
PRIMER_RIGHT_15_PENALTY=1.149895
PRIMER_LEFT_15_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_15_SEQUENCE=AACCTCCCAAGTCCTAACCAA
PRIMER_LEFT_15=624,20
PRIMER_RIGHT_15=1481,21
PRIMER_LEFT_15_TM=60.051
PRIMER_RIGHT_15_TM=59.850
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=858
PRIMER_PAIR_16_PENALTY=1.201240
PRIMER_LEFT_16_PENALTY=0.051345
PRIMER_RIGHT_16_PENALTY=1.149895
PRIMER_LEFT_16_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_16_SEQUENCE=AAACCTCCCAAGTCCTAACCA
PRIMER_LEFT_16=624,20
PRIMER_RIGHT_16=1482,21
PRIMER_LEFT_16_TM=60.051
PRIMER_RIGHT_16_TM=59.850
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=859
PRIMER_PAIR_17_PENALTY=1.264320
PRIMER_LEFT_17_PENALTY=0.288804
PRIMER_RIGHT_17_PENALTY=0.975516
PRIMER_LEFT_17_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_17_SEQUENCE=CCTCCCAAGTCCTAACCAAA
PRIMER_LEFT_17=511,20
PRIMER_RIGHT_17=1479,20
PRIMER_LEFT_17_TM=59.711
PRIMER_RIGHT_17_TM=59.024
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.8000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=969
PRIMER_PAIR_18_PENALTY=1.294734
PRIMER_LEFT_18_PENALTY=1.228411
PRIMER_RIGHT_18_PENALTY=0.066323
PRIMER_LEFT_18_SEQUENCE=AACAGCCAAAATAGGCACCC
PRIMER_RIGHT_18_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_18=499,20
PRIMER_RIGHT_18=1491,20
PRIMER_LEFT_18_TM=61.228
PRIMER_RIGHT_18_TM=60.066
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=10.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=993
PRIMER_PAIR_19_PENALTY=1.333817
PRIMER_LEFT_19_PENALTY=1.267494
PRIMER_RIGHT_19_PENALTY=0.066323
PRIMER_LEFT_19_SEQUENCE=TAGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_19_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_19=510,21
PRIMER_RIGHT_19=1491,20
PRIMER_LEFT_19_TM=59.733
PRIMER_RIGHT_19_TM=60.066
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=10.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=982
PRIMER_PAIR_20_PENALTY=1.334635
PRIMER_LEFT_20_PENALTY=0.288804
PRIMER_RIGHT_20_PENALTY=1.045831
PRIMER_LEFT_20_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_20_SEQUENCE=GTTCGATCGTAGAAACCTCCC
PRIMER_LEFT_20=511,20
PRIMER_RIGHT_20=1494,21
PRIMER_LEFT_20_TM=59.711
PRIMER_RIGHT_20_TM=59.954
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=984
PRIMER_PAIR_21_PENALTY=1.334635
PRIMER_LEFT_21_PENALTY=0.288804
PRIMER_RIGHT_21_PENALTY=1.045831
PRIMER_LEFT_21_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_21_SEQUENCE=GGTTCGATCGTAGAAACCTCC
PRIMER_LEFT_21=511,20
PRIMER_RIGHT_21=1495,21
PRIMER_LEFT_21_TM=59.711
PRIMER_RIGHT_21_TM=59.954
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=985
PRIMER_PAIR_22_PENALTY=1.370289
PRIMER_LEFT_22_PENALTY=0.495790
PRIMER_RIGHT_22_PENALTY=0.874500
PRIMER_LEFT_22_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_22_SEQUENCE=TTCGATCGTAGAAACCTCCC
PRIMER_LEFT_22=676,20
PRIMER_RIGHT_22=1493,20
PRIMER_LEFT_22_TM=59.504
PRIMER_RIGHT_22_TM=59.126
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.3000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=818
PRIMER_PAIR_23_PENALTY=1.381885
PRIMER_LEFT_23_PENALTY=0.051345
PRIMER_RIGHT_23_PENALTY=1.330540
PRIMER_LEFT_23_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_23_SEQUENCE=CCTCCCAAGTCCTAACCAAAA
PRIMER_LEFT_23=624,20
PRIMER_RIGHT_23=1479,21
PRIMER_LEFT_23_TM=60.051
PRIMER_RIGHT_23_TM=60.331
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=856
PRIMER_PAIR_24_PENALTY=1.407252
PRIMER_LEFT_24_PENALTY=0.051345
PRIMER_RIGHT_24_PENALTY=1.355907
PRIMER_LEFT_24_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_24_SEQUENCE=CGTAGAAACCTCCCAAGTCC
PRIMER_LEFT_24=624,20
PRIMER_RIGHT_24=1487,20
PRIMER_LEFT_24_TM=60.051
PRIMER_RIGHT_24_TM=58.644
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=864
PRIMER_PAIR_25_PENALTY=1.438699
PRIMER_LEFT_25_PENALTY=0.288804
PRIMER_RIGHT_25_PENALTY=1.149895
PRIMER_LEFT_25_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_25_SEQUENCE=ACCTCCCAAGTCCTAACCAAA
PRIMER_LEFT_25=511,20
PRIMER_RIGHT_25=1480,21
PRIMER_LEFT_25_TM=59.711
PRIMER_RIGHT_25_TM=59.850
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=970
PRIMER_PAIR_26_PENALTY=1.438699
PRIMER_LEFT_26_PENALTY=0.288804
PRIMER_RIGHT_26_PENALTY=1.149895
PRIMER_LEFT_26_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_26_SEQUENCE=AACCTCCCAAGTCCTAACCAA
PRIMER_LEFT_26=511,20
PRIMER_RIGHT_26=1481,21
PRIMER_LEFT_26_TM=59.711
PRIMER_RIGHT_26_TM=59.850
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=971
PRIMER_PAIR_27_PENALTY=1.438699
PRIMER_LEFT_27_PENALTY=0.288804
PRIMER_RIGHT_27_PENALTY=1.149895
PRIMER_LEFT_27_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_27_SEQUENCE=AAACCTCCCAAGTCCTAACCA
PRIMER_LEFT_27=511,20
PRIMER_RIGHT_27=1482,21
PRIMER_LEFT_27_TM=59.711
PRIMER_RIGHT_27_TM=59.850
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=972
PRIMER_PAIR_28_PENALTY=1.445622
PRIMER_LEFT_28_PENALTY=0.571122
PRIMER_RIGHT_28_PENALTY=0.874500
PRIMER_LEFT_28_SEQUENCE=CCGGCCCAATATAAAGTCTG
PRIMER_RIGHT_28_SEQUENCE=TTCGATCGTAGAAACCTCCC
PRIMER_LEFT_28=798,20
PRIMER_RIGHT_28=1493,20
PRIMER_LEFT_28_TM=59.429
PRIMER_RIGHT_28_TM=59.126
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=696
PRIMER_PAIR_29_PENALTY=1.446254
PRIMER_LEFT_29_PENALTY=1.379931
PRIMER_RIGHT_29_PENALTY=0.066323
PRIMER_LEFT_29_SEQUENCE=GAGCAGTTTCCATTTTCAGCA
PRIMER_RIGHT_29_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_29=633,21
PRIMER_RIGHT_29=1491,20
PRIMER_LEFT_29_TM=60.380
PRIMER_RIGHT_29_TM=60.066
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=10.0000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=859
PRIMER_PAIR_30_PENALTY=1.471306
PRIMER_LEFT_30_PENALTY=0.495790
PRIMER_RIGHT_30_PENALTY=0.975516
PRIMER_LEFT_30_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_30_SEQUENCE=CCTCCCAAGTCCTAACCAAA
PRIMER_LEFT_30=676,20
PRIMER_RIGHT_30=1479,20
PRIMER_LEFT_30_TM=59.504
PRIMER_RIGHT_30_TM=59.024
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.3000
PRIMER_RIGHT_30_END_STABILITY=8.8000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=804
PRIMER_PAIR_31_PENALTY=1.533008
PRIMER_LEFT_31_PENALTY=0.051345
PRIMER_RIGHT_31_PENALTY=1.481663
PRIMER_LEFT_31_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_31_SEQUENCE=TCGTAGAAACCTCCCAAGTCC
PRIMER_LEFT_31=624,20
PRIMER_RIGHT_31=1488,21
PRIMER_LEFT_31_TM=60.051
PRIMER_RIGHT_31_TM=60.482
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=865
PRIMER_PAIR_32_PENALTY=1.541621
PRIMER_LEFT_32_PENALTY=0.495790
PRIMER_RIGHT_32_PENALTY=1.045831
PRIMER_LEFT_32_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_32_SEQUENCE=GTTCGATCGTAGAAACCTCCC
PRIMER_LEFT_32=676,20
PRIMER_RIGHT_32=1494,21
PRIMER_LEFT_32_TM=59.504
PRIMER_RIGHT_32_TM=59.954
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.3000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=819
PRIMER_PAIR_33_PENALTY=1.541621
PRIMER_LEFT_33_PENALTY=0.495790
PRIMER_RIGHT_33_PENALTY=1.045831
PRIMER_LEFT_33_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_33_SEQUENCE=GGTTCGATCGTAGAAACCTCC
PRIMER_LEFT_33=676,20
PRIMER_RIGHT_33=1495,21
PRIMER_LEFT_33_TM=59.504
PRIMER_RIGHT_33_TM=59.954
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.3000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=820
PRIMER_PAIR_34_PENALTY=1.546638
PRIMER_LEFT_34_PENALTY=0.571122
PRIMER_RIGHT_34_PENALTY=0.975516
PRIMER_LEFT_34_SEQUENCE=CCGGCCCAATATAAAGTCTG
PRIMER_RIGHT_34_SEQUENCE=CCTCCCAAGTCCTAACCAAA
PRIMER_LEFT_34=798,20
PRIMER_RIGHT_34=1479,20
PRIMER_LEFT_34_TM=59.429
PRIMER_RIGHT_34_TM=59.024
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.8000
PRIMER_PAIR_34_COMPL_ANY=2.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=682
PRIMER_PAIR_35_PENALTY=1.553816
PRIMER_LEFT_35_PENALTY=0.051345
PRIMER_RIGHT_35_PENALTY=1.502471
PRIMER_LEFT_35_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_35_SEQUENCE=ACCTCCCAAGTCCTAACCAA
PRIMER_LEFT_35=624,20
PRIMER_RIGHT_35=1480,20
PRIMER_LEFT_35_TM=60.051
PRIMER_RIGHT_35_TM=58.498
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=857
PRIMER_PAIR_36_PENALTY=1.553816
PRIMER_LEFT_36_PENALTY=0.051345
PRIMER_RIGHT_36_PENALTY=1.502471
PRIMER_LEFT_36_SEQUENCE=TTCCCAAATGAGCAGTTTCC
PRIMER_RIGHT_36_SEQUENCE=AACCTCCCAAGTCCTAACCA
PRIMER_LEFT_36=624,20
PRIMER_RIGHT_36=1481,20
PRIMER_LEFT_36_TM=60.051
PRIMER_RIGHT_36_TM=58.498
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=858
PRIMER_PAIR_37_PENALTY=1.563986
PRIMER_LEFT_37_PENALTY=1.497663
PRIMER_RIGHT_37_PENALTY=0.066323
PRIMER_LEFT_37_SEQUENCE=AGCAGTTTCCATTTTCAGCA
PRIMER_RIGHT_37_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_37=634,20
PRIMER_RIGHT_37=1491,20
PRIMER_LEFT_37_TM=58.502
PRIMER_RIGHT_37_TM=60.066
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=10.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=858
PRIMER_PAIR_38_PENALTY=1.571923
PRIMER_LEFT_38_PENALTY=0.571122
PRIMER_RIGHT_38_PENALTY=1.000801
PRIMER_LEFT_38_SEQUENCE=CCGGCCCAATATAAAGTCTG
PRIMER_RIGHT_38_SEQUENCE=CCATTTCTTGAAACCGACGA
PRIMER_LEFT_38=798,20
PRIMER_RIGHT_38=1748,20
PRIMER_LEFT_38_TM=59.429
PRIMER_RIGHT_38_TM=61.001
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=951
PRIMER_PAIR_39_PENALTY=1.597844
PRIMER_LEFT_39_PENALTY=1.531521
PRIMER_RIGHT_39_PENALTY=0.066323
PRIMER_LEFT_39_SEQUENCE=TAGTCGCTGCTACACAGCCC
PRIMER_RIGHT_39_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_39=780,20
PRIMER_RIGHT_39=1491,20
PRIMER_LEFT_39_TM=61.532
PRIMER_RIGHT_39_TM=60.066
PRIMER_LEFT_39_GC_PERCENT=60.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=10.9000
PRIMER_RIGHT_39_END_STABILITY=10.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=712
PRIMER_PAIR_40_PENALTY=1.616954
PRIMER_LEFT_40_PENALTY=0.571122
PRIMER_RIGHT_40_PENALTY=1.045831
PRIMER_LEFT_40_SEQUENCE=CCGGCCCAATATAAAGTCTG
PRIMER_RIGHT_40_SEQUENCE=GTTCGATCGTAGAAACCTCCC
PRIMER_LEFT_40=798,20
PRIMER_RIGHT_40=1494,21
PRIMER_LEFT_40_TM=59.429
PRIMER_RIGHT_40_TM=59.954
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=9.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=697
PRIMER_PAIR_41_PENALTY=1.616954
PRIMER_LEFT_41_PENALTY=0.571122
PRIMER_RIGHT_41_PENALTY=1.045831
PRIMER_LEFT_41_SEQUENCE=CCGGCCCAATATAAAGTCTG
PRIMER_RIGHT_41_SEQUENCE=GGTTCGATCGTAGAAACCTCC
PRIMER_LEFT_41=798,20
PRIMER_RIGHT_41=1495,21
PRIMER_LEFT_41_TM=59.429
PRIMER_RIGHT_41_TM=59.954
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=698
PRIMER_PAIR_42_PENALTY=1.619344
PRIMER_LEFT_42_PENALTY=0.288804
PRIMER_RIGHT_42_PENALTY=1.330540
PRIMER_LEFT_42_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_42_SEQUENCE=CCTCCCAAGTCCTAACCAAAA
PRIMER_LEFT_42=511,20
PRIMER_RIGHT_42=1479,21
PRIMER_LEFT_42_TM=59.711
PRIMER_RIGHT_42_TM=60.331
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=969
PRIMER_PAIR_43_PENALTY=1.643213
PRIMER_LEFT_43_PENALTY=1.576890
PRIMER_RIGHT_43_PENALTY=0.066323
PRIMER_LEFT_43_SEQUENCE=GCCAAAATAGGCACCCAAAC
PRIMER_RIGHT_43_SEQUENCE=CGATCGTAGAAACCTCCCAA
PRIMER_LEFT_43=503,20
PRIMER_RIGHT_43=1491,20
PRIMER_LEFT_43_TM=61.577
PRIMER_RIGHT_43_TM=60.066
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=10.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=989
PRIMER_PAIR_44_PENALTY=1.644597
PRIMER_LEFT_44_PENALTY=1.051012
PRIMER_RIGHT_44_PENALTY=0.593585
PRIMER_LEFT_44_SEQUENCE=CCAAAATAGGCACCCAAACT
PRIMER_RIGHT_44_SEQUENCE=TCGATCGTAGAAACCTCCCA
PRIMER_LEFT_44=504,20
PRIMER_RIGHT_44=1492,20
PRIMER_LEFT_44_TM=58.949
PRIMER_RIGHT_44_TM=60.594
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=9.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=989
PRIMER_PAIR_45_PENALTY=1.644711
PRIMER_LEFT_45_PENALTY=0.288804
PRIMER_RIGHT_45_PENALTY=1.355907
PRIMER_LEFT_45_SEQUENCE=AGGCACCCAAACTTGAAGAA
PRIMER_RIGHT_45_SEQUENCE=CGTAGAAACCTCCCAAGTCC
PRIMER_LEFT_45=511,20
PRIMER_RIGHT_45=1487,20
PRIMER_LEFT_45_TM=59.711
PRIMER_RIGHT_45_TM=58.644
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=977
PRIMER_PAIR_46_PENALTY=1.645684
PRIMER_LEFT_46_PENALTY=0.495790
PRIMER_RIGHT_46_PENALTY=1.149895
PRIMER_LEFT_46_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_46_SEQUENCE=ACCTCCCAAGTCCTAACCAAA
PRIMER_LEFT_46=676,20
PRIMER_RIGHT_46=1480,21
PRIMER_LEFT_46_TM=59.504
PRIMER_RIGHT_46_TM=59.850
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.3000
PRIMER_RIGHT_46_END_STABILITY=8.8000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=805
PRIMER_PAIR_47_PENALTY=1.645684
PRIMER_LEFT_47_PENALTY=0.495790
PRIMER_RIGHT_47_PENALTY=1.149895
PRIMER_LEFT_47_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_47_SEQUENCE=AACCTCCCAAGTCCTAACCAA
PRIMER_LEFT_47=676,20
PRIMER_RIGHT_47=1481,21
PRIMER_LEFT_47_TM=59.504
PRIMER_RIGHT_47_TM=59.850
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.3000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=806
PRIMER_PAIR_48_PENALTY=1.645684
PRIMER_LEFT_48_PENALTY=0.495790
PRIMER_RIGHT_48_PENALTY=1.149895
PRIMER_LEFT_48_SEQUENCE=TCGCACGGATGAATAATGAC
PRIMER_RIGHT_48_SEQUENCE=AAACCTCCCAAGTCCTAACCA
PRIMER_LEFT_48=676,20
PRIMER_RIGHT_48=1482,21
PRIMER_LEFT_48_TM=59.504
PRIMER_RIGHT_48_TM=59.850
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.3000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=807
PRIMER_PAIR_49_PENALTY=1.696802
PRIMER_LEFT_49_PENALTY=1.103216
PRIMER_RIGHT_49_PENALTY=0.593585
PRIMER_LEFT_49_SEQUENCE=CATTTTCAGCAGCGAACCTAC
PRIMER_RIGHT_49_SEQUENCE=TCGATCGTAGAAACCTCCCA
PRIMER_LEFT_49=643,21
PRIMER_RIGHT_49=1492,20
PRIMER_LEFT_49_TM=59.897
PRIMER_RIGHT_49_TM=60.594
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=850
=
SEQUENCE_ID=MAGIC.287_3_22816278_22816509_edi-0_bur-0_9667_642830_TRUE
SEQUENCE_TARGET=950,331
SEQUENCE_TEMPLATE=GGCCCGTTTTGTNNTTCTTCAGCCCAATAAATCTTNAAAAGGAANAATTTTGCANGTAAAGGANGGANTAATAACTTTTAACTTTAAGATTAATTTTTAGAGTNTTTTNNNNNNNNNNTGNGAAANATGTCATTTAAATNATTAGTTTANCATTTTNGTTANTTTAAANNATGAATTTTGNNTATGNTNATTTAAATNNNNNATTTTATGTTGNTTTTCAATTTAATCANGAATTNTCGTTGAGTAANCTAATTGAGATACAAATTCTTNTTTTTCGATGCNATCNGTCGANTTAGAGACGNNNAATTANTTTAAATNNAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTTCAAGATCTAANNTTTTTTATTNGNAGAATTTCTAACNACATGTCTTAANNAANTTANTNAACNAAAATTNNTGATTAAATNNAAANNNAACATAAAATTAAAANTTTAAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTNATTCTTTAAACCAACTAAAATGGTAAAATGATAATNACATNNTNCCCTTTANCAAATTCGTAGTGTATCTATNTTCTCCAATTTCCGGATTCATTAACGAGTAATTNNNAANNNAAAAAANNNNNTCCNTTAAAGGTAAATAATCGTNAAATAAACATAAACAAATCTNGCCAGCTTGACTGTTATAAGTCCATTCGACCANAAACAAAGACTAGAGGAAGNAAACNAAAACCTTAAAACACTTTCAATAGAAGACACGCACTCACTGTCAGAAATCTACATCGAGAAACACACTNNNNNNTTCCAAAAACATCATTCATGAANAANACCACAGANAAAACGAAGAATATGGTTGATGATGATGATCGTATACTATGGGATTGTGGAAGTCCACTCTATGATTCCTACGAGCTCGTTTCTCTTACTCACATCATNGAACGCCATTTCATGTCTCTNCCTTCTCTTGCNGGCGCCNGAAAAGTTTGCTGCTCTNGAANACCGTCTGATCTGGAAGAAAAGGCCAATCGTGGGGCCAACACCGTCGTTGATNTGTGTTGTTNCTCTGATTCGACGACTGGTAAACTGTTGTGCGGTGCTTTTGGAACCAAATGGTGGAAGAGAAAGCAAAACACCGAAGCTGTCGTCGATAATTACAACAACAANAAGAAGAATATGTTTTGTCGGATCCTCTCTAAGATAAGGATTNCTACAACAAGATCTNGTTCTTCGAAGATNTGATTTTCATATCTTCTTTTTCTGTTTTTANTATTTGTTTGATCCTTCGTAAAATAATAATTAAGTATATTACTTGCTCATGTTTTTNAATTTGAAAATNNAATCGAATAAACTGTAATGAAACGGGAAATACATTTTATTGACTTATGGATTAAATGCAGAGTAATTAGTTTAAGTTGAAGAATTTCATGGATTTAATATCCATCACATCATACGTCTCTGAAACGTTTAGGCAAAGAGTGAAACGTGGATCAACATGCAATCAACGTTTACATTGGGGAATATGAGATATATAGCGATAGAATGATATAGGAGTCGTTTAGCAATTGCCATTTTACTAACAGTTCATCGGGGTGTCAGAAGAAAAATNTGAACTTCATAGAAAATCCTCAAGTGAATAACAAAGGACAAAACATGTGAAGCAATTTGAAAATTTCATGATTCTTCTGACAATATTCTAACATGNAAATTGCAAATGATTCTTGACTCAGTCAATGATTTAGGTCTTGGGAACTTGGTAAATTTGTGTAATTTGTTACTCTTTNAAAAAGTGTTGAGTATGCGNAGATGGGCTTCNTGNATAAAAGTCTGTAACGGCCCATCTCTNTAACTTTGAACAGAATTAAAGGNTATATTGTTTCCGTTAGAATTTACCTTTTCTTGTTGAGTATAGTTTAGCCGTTACNACATAAAATGCTTCCTCAATAAAGTATTAAAGCTTATGATAACAACGAGCATCTTCNAATTTGTTCCTCCATATACTTTAGTTTATATCATATAAGTAGACTATTTTTGAAACACTTATATTAACAACATTGANGGGTGTTATATTACACTCCAATTCACATCATCAAGAGTACAAAAGGCTGCTGAAGANAGAAAAGAGAGGAACAAATCACAATAATTAAAATCACANGAAAGTGTAAAATNAATGCGAAATAGACCAATAGCTATNATTGCATAATCCATT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.674615
PRIMER_LEFT_0_PENALTY=0.384026
PRIMER_RIGHT_0_PENALTY=0.290589
PRIMER_LEFT_0_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_0_SEQUENCE=CCACGTTTCACTCTTTGCCT
PRIMER_LEFT_0=599,20
PRIMER_RIGHT_0=1510,20
PRIMER_LEFT_0_TM=60.384
PRIMER_RIGHT_0_TM=60.291
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=912
PRIMER_PAIR_1_PENALTY=1.378623
PRIMER_LEFT_1_PENALTY=1.088034
PRIMER_RIGHT_1_PENALTY=0.290589
PRIMER_LEFT_1_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_1_SEQUENCE=CCACGTTTCACTCTTTGCCT
PRIMER_LEFT_1=779,21
PRIMER_RIGHT_1=1510,20
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=60.291
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=732
PRIMER_PAIR_2_PENALTY=1.598365
PRIMER_LEFT_2_PENALTY=1.088034
PRIMER_RIGHT_2_PENALTY=0.510331
PRIMER_LEFT_2_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_2_SEQUENCE=TTCTGACACCCCGATGAACT
PRIMER_LEFT_2=779,21
PRIMER_RIGHT_2=1622,20
PRIMER_LEFT_2_TM=60.088
PRIMER_RIGHT_2_TM=60.510
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=844
PRIMER_PAIR_3_PENALTY=1.598365
PRIMER_LEFT_3_PENALTY=1.088034
PRIMER_RIGHT_3_PENALTY=0.510331
PRIMER_LEFT_3_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_3_SEQUENCE=CTTCTGACACCCCGATGAAC
PRIMER_LEFT_3=779,21
PRIMER_RIGHT_3=1623,20
PRIMER_LEFT_3_TM=60.088
PRIMER_RIGHT_3_TM=60.510
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=845
PRIMER_PAIR_4_PENALTY=1.600013
PRIMER_LEFT_4_PENALTY=0.384026
PRIMER_RIGHT_4_PENALTY=1.215987
PRIMER_LEFT_4_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_4_SEQUENCE=TCCACGTTTCACTCTTTGCC
PRIMER_LEFT_4=599,20
PRIMER_RIGHT_4=1511,20
PRIMER_LEFT_4_TM=60.384
PRIMER_RIGHT_4_TM=61.216
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=913
PRIMER_PAIR_5_PENALTY=2.304021
PRIMER_LEFT_5_PENALTY=1.088034
PRIMER_RIGHT_5_PENALTY=1.215987
PRIMER_LEFT_5_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_5_SEQUENCE=TCCACGTTTCACTCTTTGCC
PRIMER_LEFT_5=779,21
PRIMER_RIGHT_5=1511,20
PRIMER_LEFT_5_TM=60.088
PRIMER_RIGHT_5_TM=61.216
PRIMER_LEFT_5_GC_PERCENT=52.381
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=10.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=733
PRIMER_PAIR_6_PENALTY=2.337015
PRIMER_LEFT_6_PENALTY=2.046426
PRIMER_RIGHT_6_PENALTY=0.290589
PRIMER_LEFT_6_SEQUENCE=ACACGCACTCACTGTCAGAA
PRIMER_RIGHT_6_SEQUENCE=CCACGTTTCACTCTTTGCCT
PRIMER_LEFT_6=780,20
PRIMER_RIGHT_6=1510,20
PRIMER_LEFT_6_TM=57.954
PRIMER_RIGHT_6_TM=60.291
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=731
PRIMER_PAIR_7_PENALTY=2.530551
PRIMER_LEFT_7_PENALTY=0.384026
PRIMER_RIGHT_7_PENALTY=2.146525
PRIMER_LEFT_7_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_7_SEQUENCE=TTGATCCACGTTTCACTCTTTG
PRIMER_LEFT_7=599,20
PRIMER_RIGHT_7=1515,22
PRIMER_LEFT_7_TM=60.384
PRIMER_RIGHT_7_TM=60.147
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=917
PRIMER_PAIR_8_PENALTY=2.556757
PRIMER_LEFT_8_PENALTY=2.046426
PRIMER_RIGHT_8_PENALTY=0.510331
PRIMER_LEFT_8_SEQUENCE=ACACGCACTCACTGTCAGAA
PRIMER_RIGHT_8_SEQUENCE=TTCTGACACCCCGATGAACT
PRIMER_LEFT_8=780,20
PRIMER_RIGHT_8=1622,20
PRIMER_LEFT_8_TM=57.954
PRIMER_RIGHT_8_TM=60.510
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=8.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=843
PRIMER_PAIR_9_PENALTY=2.556757
PRIMER_LEFT_9_PENALTY=2.046426
PRIMER_RIGHT_9_PENALTY=0.510331
PRIMER_LEFT_9_SEQUENCE=ACACGCACTCACTGTCAGAA
PRIMER_RIGHT_9_SEQUENCE=CTTCTGACACCCCGATGAAC
PRIMER_LEFT_9=780,20
PRIMER_RIGHT_9=1623,20
PRIMER_LEFT_9_TM=57.954
PRIMER_RIGHT_9_TM=60.510
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=8.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=844
PRIMER_PAIR_10_PENALTY=2.623350
PRIMER_LEFT_10_PENALTY=0.384026
PRIMER_RIGHT_10_PENALTY=2.239324
PRIMER_LEFT_10_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_10_SEQUENCE=TGATCCACGTTTCACTCTTTG
PRIMER_LEFT_10=599,20
PRIMER_RIGHT_10=1514,21
PRIMER_LEFT_10_TM=60.384
PRIMER_RIGHT_10_TM=58.761
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=916
PRIMER_PAIR_11_PENALTY=2.624940
PRIMER_LEFT_11_PENALTY=1.088034
PRIMER_RIGHT_11_PENALTY=1.536905
PRIMER_LEFT_11_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_11_SEQUENCE=GACACCCCGATGAACTGTTAG
PRIMER_LEFT_11=779,21
PRIMER_RIGHT_11=1618,21
PRIMER_LEFT_11_TM=60.088
PRIMER_RIGHT_11_TM=59.463
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=5.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=840
PRIMER_PAIR_12_PENALTY=2.698937
PRIMER_LEFT_12_PENALTY=1.088034
PRIMER_RIGHT_12_PENALTY=1.610903
PRIMER_LEFT_12_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_12_SEQUENCE=TCTTCTGACACCCCGATGAA
PRIMER_LEFT_12=779,21
PRIMER_RIGHT_12=1624,20
PRIMER_LEFT_12_TM=60.088
PRIMER_RIGHT_12_TM=61.611
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=8.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=846
PRIMER_PAIR_13_PENALTY=2.745233
PRIMER_LEFT_13_PENALTY=0.384026
PRIMER_RIGHT_13_PENALTY=2.361206
PRIMER_LEFT_13_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_13_SEQUENCE=TGTTGATCCACGTTTCACTCTT
PRIMER_LEFT_13=599,20
PRIMER_RIGHT_13=1517,22
PRIMER_LEFT_13_TM=60.384
PRIMER_RIGHT_13_TM=59.639
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=919
PRIMER_PAIR_14_PENALTY=2.748694
PRIMER_LEFT_14_PENALTY=0.384026
PRIMER_RIGHT_14_PENALTY=2.364668
PRIMER_LEFT_14_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_14_SEQUENCE=GCATGTTGATCCACGTTTCAC
PRIMER_LEFT_14=599,20
PRIMER_RIGHT_14=1520,21
PRIMER_LEFT_14_TM=60.384
PRIMER_RIGHT_14_TM=61.365
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=922
PRIMER_PAIR_15_PENALTY=2.802712
PRIMER_LEFT_15_PENALTY=0.384026
PRIMER_RIGHT_15_PENALTY=2.418685
PRIMER_LEFT_15_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_15_SEQUENCE=CATGTTGATCCACGTTTCACTC
PRIMER_LEFT_15=599,20
PRIMER_RIGHT_15=1519,22
PRIMER_LEFT_15_TM=60.384
PRIMER_RIGHT_15_TM=60.419
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=45.455
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=921
PRIMER_PAIR_16_PENALTY=2.806789
PRIMER_LEFT_16_PENALTY=0.384026
PRIMER_RIGHT_16_PENALTY=2.422763
PRIMER_LEFT_16_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_16_SEQUENCE=ATCCACGTTTCACTCTTTGCC
PRIMER_LEFT_16=599,20
PRIMER_RIGHT_16=1512,21
PRIMER_LEFT_16_TM=60.384
PRIMER_RIGHT_16_TM=61.423
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=10.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=914
PRIMER_PAIR_17_PENALTY=2.900329
PRIMER_LEFT_17_PENALTY=0.384026
PRIMER_RIGHT_17_PENALTY=2.516303
PRIMER_LEFT_17_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_17_SEQUENCE=CATGTTGATCCACGTTTCACT
PRIMER_LEFT_17=599,20
PRIMER_RIGHT_17=1519,21
PRIMER_LEFT_17_TM=60.384
PRIMER_RIGHT_17_TM=58.484
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=921
PRIMER_PAIR_18_PENALTY=3.045618
PRIMER_LEFT_18_PENALTY=0.384026
PRIMER_RIGHT_18_PENALTY=2.661591
PRIMER_LEFT_18_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_18_SEQUENCE=CATGTTGATCCACGTTTCAC
PRIMER_LEFT_18=599,20
PRIMER_RIGHT_18=1519,20
PRIMER_LEFT_18_TM=60.384
PRIMER_RIGHT_18_TM=57.338
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=921
PRIMER_PAIR_19_PENALTY=3.182985
PRIMER_LEFT_19_PENALTY=0.384026
PRIMER_RIGHT_19_PENALTY=2.798959
PRIMER_LEFT_19_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_19_SEQUENCE=TGTTGATCCACGTTTCACTCT
PRIMER_LEFT_19=599,20
PRIMER_RIGHT_19=1517,21
PRIMER_LEFT_19_TM=60.384
PRIMER_RIGHT_19_TM=58.201
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=6.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=919
PRIMER_PAIR_20_PENALTY=3.234559
PRIMER_LEFT_20_PENALTY=1.088034
PRIMER_RIGHT_20_PENALTY=2.146525
PRIMER_LEFT_20_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_20_SEQUENCE=TTGATCCACGTTTCACTCTTTG
PRIMER_LEFT_20=779,21
PRIMER_RIGHT_20=1515,22
PRIMER_LEFT_20_TM=60.088
PRIMER_RIGHT_20_TM=60.147
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=737
PRIMER_PAIR_21_PENALTY=3.262413
PRIMER_LEFT_21_PENALTY=2.046426
PRIMER_RIGHT_21_PENALTY=1.215987
PRIMER_LEFT_21_SEQUENCE=ACACGCACTCACTGTCAGAA
PRIMER_RIGHT_21_SEQUENCE=TCCACGTTTCACTCTTTGCC
PRIMER_LEFT_21=780,20
PRIMER_RIGHT_21=1511,20
PRIMER_LEFT_21_TM=57.954
PRIMER_RIGHT_21_TM=61.216
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=10.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=732
PRIMER_PAIR_22_PENALTY=3.327358
PRIMER_LEFT_22_PENALTY=1.088034
PRIMER_RIGHT_22_PENALTY=2.239324
PRIMER_LEFT_22_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_22_SEQUENCE=TGATCCACGTTTCACTCTTTG
PRIMER_LEFT_22=779,21
PRIMER_RIGHT_22=1514,21
PRIMER_LEFT_22_TM=60.088
PRIMER_RIGHT_22_TM=58.761
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=736
PRIMER_PAIR_23_PENALTY=3.350527
PRIMER_LEFT_23_PENALTY=0.384026
PRIMER_RIGHT_23_PENALTY=2.966501
PRIMER_LEFT_23_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_23_SEQUENCE=TGTTGATCCACGTTTCACTC
PRIMER_LEFT_23=599,20
PRIMER_RIGHT_23=1517,20
PRIMER_LEFT_23_TM=60.384
PRIMER_RIGHT_23_TM=57.033
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=919
PRIMER_PAIR_24_PENALTY=3.391695
PRIMER_LEFT_24_PENALTY=0.384026
PRIMER_RIGHT_24_PENALTY=3.007669
PRIMER_LEFT_24_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_24_SEQUENCE=TTCAGAGACGTATGATGTGATGG
PRIMER_LEFT_24=599,20
PRIMER_RIGHT_24=1484,23
PRIMER_LEFT_24_TM=60.384
PRIMER_RIGHT_24_TM=60.008
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=43.478
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=886
PRIMER_PAIR_25_PENALTY=3.392574
PRIMER_LEFT_25_PENALTY=3.101985
PRIMER_RIGHT_25_PENALTY=0.290589
PRIMER_LEFT_25_SEQUENCE=AGACACGCACTCACTGTCAGAA
PRIMER_RIGHT_25_SEQUENCE=CCACGTTTCACTCTTTGCCT
PRIMER_LEFT_25=778,22
PRIMER_RIGHT_25=1510,20
PRIMER_LEFT_25_TM=61.102
PRIMER_RIGHT_25_TM=60.291
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=733
PRIMER_PAIR_26_PENALTY=3.433752
PRIMER_LEFT_26_PENALTY=1.088034
PRIMER_RIGHT_26_PENALTY=2.345718
PRIMER_LEFT_26_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_26_SEQUENCE=GCTTCACATGTTTTGTCCTTTG
PRIMER_LEFT_26=779,21
PRIMER_RIGHT_26=1683,22
PRIMER_LEFT_26_TM=60.088
PRIMER_RIGHT_26_TM=59.654
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.3000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=905
PRIMER_PAIR_27_PENALTY=3.449240
PRIMER_LEFT_27_PENALTY=1.088034
PRIMER_RIGHT_27_PENALTY=2.361206
PRIMER_LEFT_27_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_27_SEQUENCE=TGTTGATCCACGTTTCACTCTT
PRIMER_LEFT_27=779,21
PRIMER_RIGHT_27=1517,22
PRIMER_LEFT_27_TM=60.088
PRIMER_RIGHT_27_TM=59.639
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=739
PRIMER_PAIR_28_PENALTY=3.452702
PRIMER_LEFT_28_PENALTY=1.088034
PRIMER_RIGHT_28_PENALTY=2.364668
PRIMER_LEFT_28_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_28_SEQUENCE=GCATGTTGATCCACGTTTCAC
PRIMER_LEFT_28=779,21
PRIMER_RIGHT_28=1520,21
PRIMER_LEFT_28_TM=60.088
PRIMER_RIGHT_28_TM=61.365
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=742
PRIMER_PAIR_29_PENALTY=3.491514
PRIMER_LEFT_29_PENALTY=0.384026
PRIMER_RIGHT_29_PENALTY=3.107488
PRIMER_LEFT_29_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_29_SEQUENCE=TCCACGTTTCACTCTTTGCCT
PRIMER_LEFT_29=599,20
PRIMER_RIGHT_29=1511,21
PRIMER_LEFT_29_TM=60.384
PRIMER_RIGHT_29_TM=62.107
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=913
PRIMER_PAIR_30_PENALTY=3.506720
PRIMER_LEFT_30_PENALTY=1.088034
PRIMER_RIGHT_30_PENALTY=2.418685
PRIMER_LEFT_30_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_30_SEQUENCE=CATGTTGATCCACGTTTCACTC
PRIMER_LEFT_30=779,21
PRIMER_RIGHT_30=1519,22
PRIMER_LEFT_30_TM=60.088
PRIMER_RIGHT_30_TM=60.419
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=741
PRIMER_PAIR_31_PENALTY=3.510797
PRIMER_LEFT_31_PENALTY=1.088034
PRIMER_RIGHT_31_PENALTY=2.422763
PRIMER_LEFT_31_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_31_SEQUENCE=ATCCACGTTTCACTCTTTGCC
PRIMER_LEFT_31=779,21
PRIMER_RIGHT_31=1512,21
PRIMER_LEFT_31_TM=60.088
PRIMER_RIGHT_31_TM=61.423
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=10.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=734
PRIMER_PAIR_32_PENALTY=3.545735
PRIMER_LEFT_32_PENALTY=1.088034
PRIMER_RIGHT_32_PENALTY=2.457701
PRIMER_LEFT_32_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_32_SEQUENCE=CTTCTGACACCCCGATGAACT
PRIMER_LEFT_32=779,21
PRIMER_RIGHT_32=1623,21
PRIMER_LEFT_32_TM=60.088
PRIMER_RIGHT_32_TM=61.458
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=8.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=845
PRIMER_PAIR_33_PENALTY=3.573602
PRIMER_LEFT_33_PENALTY=1.088034
PRIMER_RIGHT_33_PENALTY=2.485568
PRIMER_LEFT_33_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_33_SEQUENCE=ACACCCCGATGAACTGTTAG
PRIMER_LEFT_33=779,21
PRIMER_RIGHT_33=1617,20
PRIMER_LEFT_33_TM=60.088
PRIMER_RIGHT_33_TM=57.514
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=839
PRIMER_PAIR_34_PENALTY=3.583332
PRIMER_LEFT_34_PENALTY=2.046426
PRIMER_RIGHT_34_PENALTY=1.536905
PRIMER_LEFT_34_SEQUENCE=ACACGCACTCACTGTCAGAA
PRIMER_RIGHT_34_SEQUENCE=GACACCCCGATGAACTGTTAG
PRIMER_LEFT_34=780,20
PRIMER_RIGHT_34=1618,21
PRIMER_LEFT_34_TM=57.954
PRIMER_RIGHT_34_TM=59.463
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=5.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=839
PRIMER_PAIR_35_PENALTY=3.592158
PRIMER_LEFT_35_PENALTY=0.384026
PRIMER_RIGHT_35_PENALTY=3.208132
PRIMER_LEFT_35_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_35_SEQUENCE=CACGTTTCACTCTTTGCCTAAAC
PRIMER_LEFT_35=599,20
PRIMER_RIGHT_35=1509,23
PRIMER_LEFT_35_TM=60.384
PRIMER_RIGHT_35_TM=60.208
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=43.478
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=6.0000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=911
PRIMER_PAIR_36_PENALTY=3.604337
PRIMER_LEFT_36_PENALTY=1.088034
PRIMER_RIGHT_36_PENALTY=2.516303
PRIMER_LEFT_36_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_36_SEQUENCE=CATGTTGATCCACGTTTCACT
PRIMER_LEFT_36=779,21
PRIMER_RIGHT_36=1519,21
PRIMER_LEFT_36_TM=60.088
PRIMER_RIGHT_36_TM=58.484
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=741
PRIMER_PAIR_37_PENALTY=3.612316
PRIMER_LEFT_37_PENALTY=3.101985
PRIMER_RIGHT_37_PENALTY=0.510331
PRIMER_LEFT_37_SEQUENCE=AGACACGCACTCACTGTCAGAA
PRIMER_RIGHT_37_SEQUENCE=TTCTGACACCCCGATGAACT
PRIMER_LEFT_37=778,22
PRIMER_RIGHT_37=1622,20
PRIMER_LEFT_37_TM=61.102
PRIMER_RIGHT_37_TM=60.510
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=8.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=845
PRIMER_PAIR_38_PENALTY=3.612316
PRIMER_LEFT_38_PENALTY=3.101985
PRIMER_RIGHT_38_PENALTY=0.510331
PRIMER_LEFT_38_SEQUENCE=AGACACGCACTCACTGTCAGAA
PRIMER_RIGHT_38_SEQUENCE=CTTCTGACACCCCGATGAAC
PRIMER_LEFT_38=778,22
PRIMER_RIGHT_38=1623,20
PRIMER_LEFT_38_TM=61.102
PRIMER_RIGHT_38_TM=60.510
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=8.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=846
PRIMER_PAIR_39_PENALTY=3.657329
PRIMER_LEFT_39_PENALTY=2.046426
PRIMER_RIGHT_39_PENALTY=1.610903
PRIMER_LEFT_39_SEQUENCE=ACACGCACTCACTGTCAGAA
PRIMER_RIGHT_39_SEQUENCE=TCTTCTGACACCCCGATGAA
PRIMER_LEFT_39=780,20
PRIMER_RIGHT_39=1624,20
PRIMER_LEFT_39_TM=57.954
PRIMER_RIGHT_39_TM=61.611
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=8.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=845
PRIMER_PAIR_40_PENALTY=3.710509
PRIMER_LEFT_40_PENALTY=3.419920
PRIMER_RIGHT_40_PENALTY=0.290589
PRIMER_LEFT_40_SEQUENCE=TTCAATAGAAGACACGCACTCA
PRIMER_RIGHT_40_SEQUENCE=CCACGTTTCACTCTTTGCCT
PRIMER_LEFT_40=769,22
PRIMER_RIGHT_40=1510,20
PRIMER_LEFT_40_TM=58.580
PRIMER_RIGHT_40_TM=60.291
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=742
PRIMER_PAIR_41_PENALTY=3.749625
PRIMER_LEFT_41_PENALTY=1.088034
PRIMER_RIGHT_41_PENALTY=2.661591
PRIMER_LEFT_41_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_41_SEQUENCE=CATGTTGATCCACGTTTCAC
PRIMER_LEFT_41=779,21
PRIMER_RIGHT_41=1519,20
PRIMER_LEFT_41_TM=60.088
PRIMER_RIGHT_41_TM=57.338
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=741
PRIMER_PAIR_42_PENALTY=3.786572
PRIMER_LEFT_42_PENALTY=0.384026
PRIMER_RIGHT_42_PENALTY=3.402545
PRIMER_LEFT_42_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_42_SEQUENCE=CAGAGACGTATGATGTGATGGA
PRIMER_LEFT_42=599,20
PRIMER_RIGHT_42=1482,22
PRIMER_LEFT_42_TM=60.384
PRIMER_RIGHT_42_TM=58.597
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=8.1000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=884
PRIMER_PAIR_43_PENALTY=3.786572
PRIMER_LEFT_43_PENALTY=0.384026
PRIMER_RIGHT_43_PENALTY=3.402545
PRIMER_LEFT_43_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_43_SEQUENCE=TCAGAGACGTATGATGTGATGG
PRIMER_LEFT_43=599,20
PRIMER_RIGHT_43=1483,22
PRIMER_LEFT_43_TM=60.384
PRIMER_RIGHT_43_TM=58.597
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=8.1000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=885
PRIMER_PAIR_44_PENALTY=3.806945
PRIMER_LEFT_44_PENALTY=0.384026
PRIMER_RIGHT_44_PENALTY=3.422919
PRIMER_LEFT_44_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_44_SEQUENCE=ATGTTGATCCACGTTTCACTCT
PRIMER_LEFT_44=599,20
PRIMER_RIGHT_44=1518,22
PRIMER_LEFT_44_TM=60.384
PRIMER_RIGHT_44_TM=58.577
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=6.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=920
PRIMER_PAIR_45_PENALTY=3.852313
PRIMER_LEFT_45_PENALTY=3.561724
PRIMER_RIGHT_45_PENALTY=0.290589
PRIMER_LEFT_45_SEQUENCE=TTCAATAGAAGACACGCACTCAC
PRIMER_RIGHT_45_SEQUENCE=CCACGTTTCACTCTTTGCCT
PRIMER_LEFT_45=769,23
PRIMER_RIGHT_45=1510,20
PRIMER_LEFT_45_TM=59.438
PRIMER_RIGHT_45_TM=60.291
PRIMER_LEFT_45_GC_PERCENT=43.478
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=742
PRIMER_PAIR_46_PENALTY=3.886993
PRIMER_LEFT_46_PENALTY=1.088034
PRIMER_RIGHT_46_PENALTY=2.798959
PRIMER_LEFT_46_SEQUENCE=GACACGCACTCACTGTCAGAA
PRIMER_RIGHT_46_SEQUENCE=TGTTGATCCACGTTTCACTCT
PRIMER_LEFT_46=779,21
PRIMER_RIGHT_46=1517,21
PRIMER_LEFT_46_TM=60.088
PRIMER_RIGHT_46_TM=58.201
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.1000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=739
PRIMER_PAIR_47_PENALTY=3.888764
PRIMER_LEFT_47_PENALTY=0.384026
PRIMER_RIGHT_47_PENALTY=3.504738
PRIMER_LEFT_47_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_47_SEQUENCE=ATGTTGATCCACGTTTCACTC
PRIMER_LEFT_47=599,20
PRIMER_RIGHT_47=1518,21
PRIMER_LEFT_47_TM=60.384
PRIMER_RIGHT_47_TM=57.495
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=920
PRIMER_PAIR_48_PENALTY=3.916047
PRIMER_LEFT_48_PENALTY=0.384026
PRIMER_RIGHT_48_PENALTY=3.532021
PRIMER_LEFT_48_SEQUENCE=TTCTCCAATTTCCGGATTCA
PRIMER_RIGHT_48_SEQUENCE=TCAGAGACGTATGATGTGATGGA
PRIMER_LEFT_48=599,20
PRIMER_RIGHT_48=1483,23
PRIMER_LEFT_48_TM=60.384
PRIMER_RIGHT_48_TM=60.532
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=43.478
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=885
PRIMER_PAIR_49_PENALTY=3.930251
PRIMER_LEFT_49_PENALTY=3.419920
PRIMER_RIGHT_49_PENALTY=0.510331
PRIMER_LEFT_49_SEQUENCE=TTCAATAGAAGACACGCACTCA
PRIMER_RIGHT_49_SEQUENCE=TTCTGACACCCCGATGAACT
PRIMER_LEFT_49=769,22
PRIMER_RIGHT_49=1622,20
PRIMER_LEFT_49_TM=58.580
PRIMER_RIGHT_49_TM=60.510
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=854
=
SEQUENCE_ID=MAGIC.329_3_13891092_13891093_tsu-0_ct-1_218485_80346_TRUE
SEQUENCE_TARGET=950,101
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14067816_14067993_ler-0_po-0_30152_260864_TRUE
SEQUENCE_TARGET=950,277
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_853518_853685_wil-2_hi-0_205990_72270_TRUE
SEQUENCE_TARGET=950,267
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAGTCGCCNATGCGTTGCACTACTGCATGGGCNTNGCTCANCCCGCCANTTTGAAANCCCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGTTNAANNNNTACTTTGCGTCGATACTTATCATTAACCACTCTTTAGGTGCCACGTGTGGAATATGTCNNNCCTCAATCAAATTCAAAGGTGAAATTTTTACACGGCGCCGTCTCCTTGATCTNGCCGTGGACCGTCGTCATTGATCACAATTTAGTGATTCTCTTGGAGCGGTNTTAGATGATGAATTGATTTGCTTTTGTTATGTTTATGTGGNGCTTNTTCGTTTTGTGTTTANGAAAGAGACACAAAGAAGNGNNNNNNNTNTNTTTTTTNTTTTTCCAAAGAAGAGACTTTGTTTTCGATGTTTTGAATGTTGATTNATGGGCACATTGCCACATTCCTGTTGATTTCATTGAATTGGATANTTTAGAGAGACAGAGATTTCAGACGAAACTGGACTACTTANGACNTCTAAACAATGGGTTCTTCAAAAAAGAGTGTAGAGTTTATGATCTTCTTGAAATAACTGGTAATGGTTTCACTCGGGGCAATTGAACATTAAACGTTACTTGGTAATCAGAGGCAGANCACTGTGATTGTTATCTTTGTGAAAATCGAGTGATNTTTCCTTTAAAGNTTNATNTTTCTCATGTTATGATANACNNNNGNGTGCTTTAAAAAGATTTGATTTTCTTTATGTTGGCATATGATCATTATTTCCTTCTCTCTGTGGAAGATATTAGGAACTGACACATAGCTAGAGACAGTCAAGNATCCTAGTTTTGATGTACTGTTACNACTTTGTTGTCAGATAATATAAGATTGTTATTTCTATAATCATCATCTGTTCCTGAACCTATGTTTGGNNTTTTTTTGTATTCTTTTGGTAACATGAAGTAATGGAGATGCAGCAAACGACCACACAAGANTCTTCTCCTTCTCTTGGTGGGCTCCTGAGGAACGAACCCNTTTCACATCAAGACAGTGAATGTTTCACCNCAAANTCTATGGAATTAAATAGAAACTTCTCTCAGCCATTTTTTGTCATGTGGGATCAGAGTCTCATCTCATTACCAACTTGCCTTTATTCACTTGACGAAAGAGAGCAAGCGGATGCTTTGAGGCAATGTCCAACCAAAGAATTCAANGATGAGTAATCTTGTTTTGCTTTCNTAACACAAAATGTNTTCTTGCTTTTGTTGTTCTTCTCTCATCCACATATGAAAACTAAGTAGTTAGATGATTAGATTTNTATATATAGTGTGGTCAATTATTTTCTCATATCTTTTGAGAAGGGTACAATACTTATCTTCAAGATCTGTATGTTTCTTNGTTTTAAGCCCATACTAGCTATACTAGCTTTGATGTCATCATCTTGGTTTCATTTATGTNTTTTCTTGAAATCTAATTAGCCCTTTTTGAAATATCTTACAGAGAAATATCAGTGAACTGGATAAGANCATTTGGGGTAAATTCGATCATTTCCCTTATGAGTAGGCTACNNAGACANACATCNNNNNNNNTTCTTTNCATTTATTGTCTTAGTAANGCACTTTGGTTCTTTTCTCTNATATTNTCAACNANCNATTNNNNTTTTGTCTACCTNANTTTTNTTTCAGACNNATACATGGCCTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.011891
PRIMER_LEFT_0_PENALTY=0.516718
PRIMER_RIGHT_0_PENALTY=0.495173
PRIMER_LEFT_0_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_0_SEQUENCE=CTTTGGTTGGACATTGCCTC
PRIMER_LEFT_0=918,20
PRIMER_RIGHT_0=1670,20
PRIMER_LEFT_0_TM=60.517
PRIMER_RIGHT_0_TM=60.495
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=753
PRIMER_PAIR_1_PENALTY=1.011891
PRIMER_LEFT_1_PENALTY=0.516718
PRIMER_RIGHT_1_PENALTY=0.495173
PRIMER_LEFT_1_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_1_SEQUENCE=TCTTTGGTTGGACATTGCCT
PRIMER_LEFT_1=918,20
PRIMER_RIGHT_1=1671,20
PRIMER_LEFT_1_TM=60.517
PRIMER_RIGHT_1_TM=60.495
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=754
PRIMER_PAIR_2_PENALTY=1.075330
PRIMER_LEFT_2_PENALTY=0.580157
PRIMER_RIGHT_2_PENALTY=0.495173
PRIMER_LEFT_2_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_2_SEQUENCE=CTTTGGTTGGACATTGCCTC
PRIMER_LEFT_2=916,20
PRIMER_RIGHT_2=1670,20
PRIMER_LEFT_2_TM=59.420
PRIMER_RIGHT_2_TM=60.495
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=755
PRIMER_PAIR_3_PENALTY=1.075330
PRIMER_LEFT_3_PENALTY=0.580157
PRIMER_RIGHT_3_PENALTY=0.495173
PRIMER_LEFT_3_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_3_SEQUENCE=TCTTTGGTTGGACATTGCCT
PRIMER_LEFT_3=916,20
PRIMER_RIGHT_3=1671,20
PRIMER_LEFT_3_TM=59.420
PRIMER_RIGHT_3_TM=60.495
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=756
PRIMER_PAIR_4_PENALTY=1.129529
PRIMER_LEFT_4_PENALTY=0.516718
PRIMER_RIGHT_4_PENALTY=0.612811
PRIMER_LEFT_4_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_4_SEQUENCE=GGACATTGCCTCAAAGCATC
PRIMER_LEFT_4=918,20
PRIMER_RIGHT_4=1662,20
PRIMER_LEFT_4_TM=60.517
PRIMER_RIGHT_4_TM=60.613
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=745
PRIMER_PAIR_5_PENALTY=1.192968
PRIMER_LEFT_5_PENALTY=0.580157
PRIMER_RIGHT_5_PENALTY=0.612811
PRIMER_LEFT_5_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_5_SEQUENCE=GGACATTGCCTCAAAGCATC
PRIMER_LEFT_5=916,20
PRIMER_RIGHT_5=1662,20
PRIMER_LEFT_5_TM=59.420
PRIMER_RIGHT_5_TM=60.613
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=747
PRIMER_PAIR_6_PENALTY=1.422596
PRIMER_LEFT_6_PENALTY=0.516718
PRIMER_RIGHT_6_PENALTY=0.905878
PRIMER_LEFT_6_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_6_SEQUENCE=TCTTGTGTGGTCGTTTGCTG
PRIMER_LEFT_6=918,20
PRIMER_RIGHT_6=1458,20
PRIMER_LEFT_6_TM=60.517
PRIMER_RIGHT_6_TM=60.906
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=541
PRIMER_PAIR_7_PENALTY=1.486035
PRIMER_LEFT_7_PENALTY=0.580157
PRIMER_RIGHT_7_PENALTY=0.905878
PRIMER_LEFT_7_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_7_SEQUENCE=TCTTGTGTGGTCGTTTGCTG
PRIMER_LEFT_7=916,20
PRIMER_RIGHT_7=1458,20
PRIMER_LEFT_7_TM=59.420
PRIMER_RIGHT_7_TM=60.906
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=543
PRIMER_PAIR_8_PENALTY=1.519385
PRIMER_LEFT_8_PENALTY=1.024212
PRIMER_RIGHT_8_PENALTY=0.495173
PRIMER_LEFT_8_SEQUENCE=TGCCACATTCCTGTTGATTT
PRIMER_RIGHT_8_SEQUENCE=CTTTGGTTGGACATTGCCTC
PRIMER_LEFT_8=921,20
PRIMER_RIGHT_8=1670,20
PRIMER_LEFT_8_TM=58.976
PRIMER_RIGHT_8_TM=60.495
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=750
PRIMER_PAIR_9_PENALTY=1.519385
PRIMER_LEFT_9_PENALTY=1.024212
PRIMER_RIGHT_9_PENALTY=0.495173
PRIMER_LEFT_9_SEQUENCE=TGCCACATTCCTGTTGATTT
PRIMER_RIGHT_9_SEQUENCE=TCTTTGGTTGGACATTGCCT
PRIMER_LEFT_9=921,20
PRIMER_RIGHT_9=1671,20
PRIMER_LEFT_9_TM=58.976
PRIMER_RIGHT_9_TM=60.495
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=751
PRIMER_PAIR_10_PENALTY=1.519385
PRIMER_LEFT_10_PENALTY=1.024212
PRIMER_RIGHT_10_PENALTY=0.495173
PRIMER_LEFT_10_SEQUENCE=TTGCCACATTCCTGTTGATT
PRIMER_RIGHT_10_SEQUENCE=CTTTGGTTGGACATTGCCTC
PRIMER_LEFT_10=920,20
PRIMER_RIGHT_10=1670,20
PRIMER_LEFT_10_TM=58.976
PRIMER_RIGHT_10_TM=60.495
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=751
PRIMER_PAIR_11_PENALTY=1.519385
PRIMER_LEFT_11_PENALTY=1.024212
PRIMER_RIGHT_11_PENALTY=0.495173
PRIMER_LEFT_11_SEQUENCE=TTGCCACATTCCTGTTGATT
PRIMER_RIGHT_11_SEQUENCE=TCTTTGGTTGGACATTGCCT
PRIMER_LEFT_11=920,20
PRIMER_RIGHT_11=1671,20
PRIMER_LEFT_11_TM=58.976
PRIMER_RIGHT_11_TM=60.495
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=752
PRIMER_PAIR_12_PENALTY=1.637022
PRIMER_LEFT_12_PENALTY=1.024212
PRIMER_RIGHT_12_PENALTY=0.612811
PRIMER_LEFT_12_SEQUENCE=TTGCCACATTCCTGTTGATT
PRIMER_RIGHT_12_SEQUENCE=GGACATTGCCTCAAAGCATC
PRIMER_LEFT_12=920,20
PRIMER_RIGHT_12=1662,20
PRIMER_LEFT_12_TM=58.976
PRIMER_RIGHT_12_TM=60.613
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=743
PRIMER_PAIR_13_PENALTY=1.653798
PRIMER_LEFT_13_PENALTY=0.516718
PRIMER_RIGHT_13_PENALTY=1.137080
PRIMER_LEFT_13_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_13_SEQUENCE=CCCACCAAGAGAAGGAGAAG
PRIMER_LEFT_13=918,20
PRIMER_RIGHT_13=1480,20
PRIMER_LEFT_13_TM=60.517
PRIMER_RIGHT_13_TM=58.863
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=563
PRIMER_PAIR_14_PENALTY=1.717238
PRIMER_LEFT_14_PENALTY=0.580157
PRIMER_RIGHT_14_PENALTY=1.137080
PRIMER_LEFT_14_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_14_SEQUENCE=CCCACCAAGAGAAGGAGAAG
PRIMER_LEFT_14=916,20
PRIMER_RIGHT_14=1480,20
PRIMER_LEFT_14_TM=59.420
PRIMER_RIGHT_14_TM=58.863
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=565
PRIMER_PAIR_15_PENALTY=1.815225
PRIMER_LEFT_15_PENALTY=0.516718
PRIMER_RIGHT_15_PENALTY=1.298507
PRIMER_LEFT_15_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_15_SEQUENCE=AGCCCACCAAGAGAAGGAGA
PRIMER_LEFT_15=918,20
PRIMER_RIGHT_15=1482,20
PRIMER_LEFT_15_TM=60.517
PRIMER_RIGHT_15_TM=61.299
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=565
PRIMER_PAIR_16_PENALTY=1.815225
PRIMER_LEFT_16_PENALTY=0.516718
PRIMER_RIGHT_16_PENALTY=1.298507
PRIMER_LEFT_16_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_16_SEQUENCE=GAGCCCACCAAGAGAAGGAG
PRIMER_LEFT_16=918,20
PRIMER_RIGHT_16=1483,20
PRIMER_LEFT_16_TM=60.517
PRIMER_RIGHT_16_TM=61.299
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=60.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=566
PRIMER_PAIR_17_PENALTY=1.878664
PRIMER_LEFT_17_PENALTY=0.580157
PRIMER_RIGHT_17_PENALTY=1.298507
PRIMER_LEFT_17_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_17_SEQUENCE=AGCCCACCAAGAGAAGGAGA
PRIMER_LEFT_17=916,20
PRIMER_RIGHT_17=1482,20
PRIMER_LEFT_17_TM=59.420
PRIMER_RIGHT_17_TM=61.299
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=567
PRIMER_PAIR_18_PENALTY=1.878664
PRIMER_LEFT_18_PENALTY=0.580157
PRIMER_RIGHT_18_PENALTY=1.298507
PRIMER_LEFT_18_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_18_SEQUENCE=GAGCCCACCAAGAGAAGGAG
PRIMER_LEFT_18=916,20
PRIMER_RIGHT_18=1483,20
PRIMER_LEFT_18_TM=59.420
PRIMER_RIGHT_18_TM=61.299
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=60.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=568
PRIMER_PAIR_19_PENALTY=1.930089
PRIMER_LEFT_19_PENALTY=1.024212
PRIMER_RIGHT_19_PENALTY=0.905878
PRIMER_LEFT_19_SEQUENCE=TGCCACATTCCTGTTGATTT
PRIMER_RIGHT_19_SEQUENCE=TCTTGTGTGGTCGTTTGCTG
PRIMER_LEFT_19=921,20
PRIMER_RIGHT_19=1458,20
PRIMER_LEFT_19_TM=58.976
PRIMER_RIGHT_19_TM=60.906
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=538
PRIMER_PAIR_20_PENALTY=1.930089
PRIMER_LEFT_20_PENALTY=1.024212
PRIMER_RIGHT_20_PENALTY=0.905878
PRIMER_LEFT_20_SEQUENCE=TTGCCACATTCCTGTTGATT
PRIMER_RIGHT_20_SEQUENCE=TCTTGTGTGGTCGTTTGCTG
PRIMER_LEFT_20=920,20
PRIMER_RIGHT_20=1458,20
PRIMER_LEFT_20_TM=58.976
PRIMER_RIGHT_20_TM=60.906
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=539
PRIMER_PAIR_21_PENALTY=1.936426
PRIMER_LEFT_21_PENALTY=0.516718
PRIMER_RIGHT_21_PENALTY=1.419708
PRIMER_LEFT_21_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_21_SEQUENCE=ATCCGCTTGCTCTCTTTCGT
PRIMER_LEFT_21=918,20
PRIMER_RIGHT_21=1645,20
PRIMER_LEFT_21_TM=60.517
PRIMER_RIGHT_21_TM=61.420
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=2.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=728
PRIMER_PAIR_22_PENALTY=1.999865
PRIMER_LEFT_22_PENALTY=0.580157
PRIMER_RIGHT_22_PENALTY=1.419708
PRIMER_LEFT_22_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_22_SEQUENCE=ATCCGCTTGCTCTCTTTCGT
PRIMER_LEFT_22=916,20
PRIMER_RIGHT_22=1645,20
PRIMER_LEFT_22_TM=59.420
PRIMER_RIGHT_22_TM=61.420
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=730
PRIMER_PAIR_23_PENALTY=2.033478
PRIMER_LEFT_23_PENALTY=1.538305
PRIMER_RIGHT_23_PENALTY=0.495173
PRIMER_LEFT_23_SEQUENCE=TTAGTGATTCTCTTGGAGCGG
PRIMER_RIGHT_23_SEQUENCE=CTTTGGTTGGACATTGCCTC
PRIMER_LEFT_23=741,21
PRIMER_RIGHT_23=1670,20
PRIMER_LEFT_23_TM=59.462
PRIMER_RIGHT_23_TM=60.495
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=11.4000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=930
PRIMER_PAIR_24_PENALTY=2.033478
PRIMER_LEFT_24_PENALTY=1.538305
PRIMER_RIGHT_24_PENALTY=0.495173
PRIMER_LEFT_24_SEQUENCE=TTAGTGATTCTCTTGGAGCGG
PRIMER_RIGHT_24_SEQUENCE=TCTTTGGTTGGACATTGCCT
PRIMER_LEFT_24=741,21
PRIMER_RIGHT_24=1671,20
PRIMER_LEFT_24_TM=59.462
PRIMER_RIGHT_24_TM=60.495
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=11.4000
PRIMER_RIGHT_24_END_STABILITY=9.7000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=931
PRIMER_PAIR_25_PENALTY=2.067999
PRIMER_LEFT_25_PENALTY=0.516718
PRIMER_RIGHT_25_PENALTY=1.551281
PRIMER_LEFT_25_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_25_SEQUENCE=AGCATCCGCTTGCTCTCTTT
PRIMER_LEFT_25=918,20
PRIMER_RIGHT_25=1648,20
PRIMER_LEFT_25_TM=60.517
PRIMER_RIGHT_25_TM=61.551
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=731
PRIMER_PAIR_26_PENALTY=2.067999
PRIMER_LEFT_26_PENALTY=0.516718
PRIMER_RIGHT_26_PENALTY=1.551281
PRIMER_LEFT_26_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_26_SEQUENCE=AAGCATCCGCTTGCTCTCTT
PRIMER_LEFT_26=918,20
PRIMER_RIGHT_26=1649,20
PRIMER_LEFT_26_TM=60.517
PRIMER_RIGHT_26_TM=61.551
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=732
PRIMER_PAIR_27_PENALTY=2.082156
PRIMER_LEFT_27_PENALTY=0.516718
PRIMER_RIGHT_27_PENALTY=1.565438
PRIMER_LEFT_27_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_27_SEQUENCE=TGCTGCATCTCCATTACTTCA
PRIMER_LEFT_27=918,20
PRIMER_RIGHT_27=1443,21
PRIMER_LEFT_27_TM=60.517
PRIMER_RIGHT_27_TM=59.435
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=526
PRIMER_PAIR_28_PENALTY=2.131438
PRIMER_LEFT_28_PENALTY=0.580157
PRIMER_RIGHT_28_PENALTY=1.551281
PRIMER_LEFT_28_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_28_SEQUENCE=AGCATCCGCTTGCTCTCTTT
PRIMER_LEFT_28=916,20
PRIMER_RIGHT_28=1648,20
PRIMER_LEFT_28_TM=59.420
PRIMER_RIGHT_28_TM=61.551
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=733
PRIMER_PAIR_29_PENALTY=2.131438
PRIMER_LEFT_29_PENALTY=0.580157
PRIMER_RIGHT_29_PENALTY=1.551281
PRIMER_LEFT_29_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_29_SEQUENCE=AAGCATCCGCTTGCTCTCTT
PRIMER_LEFT_29=916,20
PRIMER_RIGHT_29=1649,20
PRIMER_LEFT_29_TM=59.420
PRIMER_RIGHT_29_TM=61.551
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=734
PRIMER_PAIR_30_PENALTY=2.145596
PRIMER_LEFT_30_PENALTY=0.580157
PRIMER_RIGHT_30_PENALTY=1.565438
PRIMER_LEFT_30_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_30_SEQUENCE=TGCTGCATCTCCATTACTTCA
PRIMER_LEFT_30=916,20
PRIMER_RIGHT_30=1443,21
PRIMER_LEFT_30_TM=59.420
PRIMER_RIGHT_30_TM=59.435
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=528
PRIMER_PAIR_31_PENALTY=2.151116
PRIMER_LEFT_31_PENALTY=1.538305
PRIMER_RIGHT_31_PENALTY=0.612811
PRIMER_LEFT_31_SEQUENCE=TTAGTGATTCTCTTGGAGCGG
PRIMER_RIGHT_31_SEQUENCE=GGACATTGCCTCAAAGCATC
PRIMER_LEFT_31=741,21
PRIMER_RIGHT_31=1662,20
PRIMER_LEFT_31_TM=59.462
PRIMER_RIGHT_31_TM=60.613
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=11.4000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=922
PRIMER_PAIR_32_PENALTY=2.161292
PRIMER_LEFT_32_PENALTY=1.024212
PRIMER_RIGHT_32_PENALTY=1.137080
PRIMER_LEFT_32_SEQUENCE=TGCCACATTCCTGTTGATTT
PRIMER_RIGHT_32_SEQUENCE=CCCACCAAGAGAAGGAGAAG
PRIMER_LEFT_32=921,20
PRIMER_RIGHT_32=1480,20
PRIMER_LEFT_32_TM=58.976
PRIMER_RIGHT_32_TM=58.863
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=560
PRIMER_PAIR_33_PENALTY=2.161292
PRIMER_LEFT_33_PENALTY=1.024212
PRIMER_RIGHT_33_PENALTY=1.137080
PRIMER_LEFT_33_SEQUENCE=TTGCCACATTCCTGTTGATT
PRIMER_RIGHT_33_SEQUENCE=CCCACCAAGAGAAGGAGAAG
PRIMER_LEFT_33=920,20
PRIMER_RIGHT_33=1480,20
PRIMER_LEFT_33_TM=58.976
PRIMER_RIGHT_33_TM=58.863
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=561
PRIMER_PAIR_34_PENALTY=2.169008
PRIMER_LEFT_34_PENALTY=0.516718
PRIMER_RIGHT_34_PENALTY=1.652290
PRIMER_LEFT_34_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_34_SEQUENCE=CGTTTGCTGCATCTCCATTAC
PRIMER_LEFT_34=918,20
PRIMER_RIGHT_34=1447,21
PRIMER_LEFT_34_TM=60.517
PRIMER_RIGHT_34_TM=60.652
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=5.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=530
PRIMER_PAIR_35_PENALTY=2.186801
PRIMER_LEFT_35_PENALTY=0.516718
PRIMER_RIGHT_35_PENALTY=1.670083
PRIMER_LEFT_35_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_35_SEQUENCE=GCCCACCAAGAGAAGGAGAA
PRIMER_LEFT_35=918,20
PRIMER_RIGHT_35=1481,20
PRIMER_LEFT_35_TM=60.517
PRIMER_RIGHT_35_TM=61.670
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=564
PRIMER_PAIR_36_PENALTY=2.232447
PRIMER_LEFT_36_PENALTY=0.580157
PRIMER_RIGHT_36_PENALTY=1.652290
PRIMER_LEFT_36_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_36_SEQUENCE=CGTTTGCTGCATCTCCATTAC
PRIMER_LEFT_36=916,20
PRIMER_RIGHT_36=1447,21
PRIMER_LEFT_36_TM=59.420
PRIMER_RIGHT_36_TM=60.652
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=5.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=532
PRIMER_PAIR_37_PENALTY=2.242344
PRIMER_LEFT_37_PENALTY=0.516718
PRIMER_RIGHT_37_PENALTY=1.725626
PRIMER_LEFT_37_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_37_SEQUENCE=TCGTTTGCTGCATCTCCATT
PRIMER_LEFT_37=918,20
PRIMER_RIGHT_37=1448,20
PRIMER_LEFT_37_TM=60.517
PRIMER_RIGHT_37_TM=61.726
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=531
PRIMER_PAIR_38_PENALTY=2.250240
PRIMER_LEFT_38_PENALTY=0.580157
PRIMER_RIGHT_38_PENALTY=1.670083
PRIMER_LEFT_38_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_38_SEQUENCE=GCCCACCAAGAGAAGGAGAA
PRIMER_LEFT_38=916,20
PRIMER_RIGHT_38=1481,20
PRIMER_LEFT_38_TM=59.420
PRIMER_RIGHT_38_TM=61.670
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=566
PRIMER_PAIR_39_PENALTY=2.257689
PRIMER_LEFT_39_PENALTY=0.516718
PRIMER_RIGHT_39_PENALTY=1.740971
PRIMER_LEFT_39_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_39_SEQUENCE=CCCACCAAGAGAAGGAGAAGA
PRIMER_LEFT_39=918,20
PRIMER_RIGHT_39=1480,21
PRIMER_LEFT_39_TM=60.517
PRIMER_RIGHT_39_TM=60.741
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=563
PRIMER_PAIR_40_PENALTY=2.305784
PRIMER_LEFT_40_PENALTY=0.580157
PRIMER_RIGHT_40_PENALTY=1.725626
PRIMER_LEFT_40_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_40_SEQUENCE=TCGTTTGCTGCATCTCCATT
PRIMER_LEFT_40=916,20
PRIMER_RIGHT_40=1448,20
PRIMER_LEFT_40_TM=59.420
PRIMER_RIGHT_40_TM=61.726
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=533
PRIMER_PAIR_41_PENALTY=2.321129
PRIMER_LEFT_41_PENALTY=0.580157
PRIMER_RIGHT_41_PENALTY=1.740971
PRIMER_LEFT_41_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_41_SEQUENCE=CCCACCAAGAGAAGGAGAAGA
PRIMER_LEFT_41=916,20
PRIMER_RIGHT_41=1480,21
PRIMER_LEFT_41_TM=59.420
PRIMER_RIGHT_41_TM=60.741
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=565
PRIMER_PAIR_42_PENALTY=2.322719
PRIMER_LEFT_42_PENALTY=1.024212
PRIMER_RIGHT_42_PENALTY=1.298507
PRIMER_LEFT_42_SEQUENCE=TGCCACATTCCTGTTGATTT
PRIMER_RIGHT_42_SEQUENCE=AGCCCACCAAGAGAAGGAGA
PRIMER_LEFT_42=921,20
PRIMER_RIGHT_42=1482,20
PRIMER_LEFT_42_TM=58.976
PRIMER_RIGHT_42_TM=61.299
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=562
PRIMER_PAIR_43_PENALTY=2.322719
PRIMER_LEFT_43_PENALTY=1.024212
PRIMER_RIGHT_43_PENALTY=1.298507
PRIMER_LEFT_43_SEQUENCE=TGCCACATTCCTGTTGATTT
PRIMER_RIGHT_43_SEQUENCE=GAGCCCACCAAGAGAAGGAG
PRIMER_LEFT_43=921,20
PRIMER_RIGHT_43=1483,20
PRIMER_LEFT_43_TM=58.976
PRIMER_RIGHT_43_TM=61.299
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=60.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=563
PRIMER_PAIR_44_PENALTY=2.322719
PRIMER_LEFT_44_PENALTY=1.024212
PRIMER_RIGHT_44_PENALTY=1.298507
PRIMER_LEFT_44_SEQUENCE=TTGCCACATTCCTGTTGATT
PRIMER_RIGHT_44_SEQUENCE=AGCCCACCAAGAGAAGGAGA
PRIMER_LEFT_44=920,20
PRIMER_RIGHT_44=1482,20
PRIMER_LEFT_44_TM=58.976
PRIMER_RIGHT_44_TM=61.299
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=563
PRIMER_PAIR_45_PENALTY=2.322719
PRIMER_LEFT_45_PENALTY=1.024212
PRIMER_RIGHT_45_PENALTY=1.298507
PRIMER_LEFT_45_SEQUENCE=TTGCCACATTCCTGTTGATT
PRIMER_RIGHT_45_SEQUENCE=GAGCCCACCAAGAGAAGGAG
PRIMER_LEFT_45=920,20
PRIMER_RIGHT_45=1483,20
PRIMER_LEFT_45_TM=58.976
PRIMER_RIGHT_45_TM=61.299
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=60.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=564
PRIMER_PAIR_46_PENALTY=2.332695
PRIMER_LEFT_46_PENALTY=0.580157
PRIMER_RIGHT_46_PENALTY=1.752538
PRIMER_LEFT_46_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_46_SEQUENCE=CGCTTGCTCTCTTTCGTCAA
PRIMER_LEFT_46=916,20
PRIMER_RIGHT_46=1642,20
PRIMER_LEFT_46_TM=59.420
PRIMER_RIGHT_46_TM=61.753
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=727
PRIMER_PAIR_47_PENALTY=2.342008
PRIMER_LEFT_47_PENALTY=0.516718
PRIMER_RIGHT_47_PENALTY=1.825290
PRIMER_LEFT_47_SEQUENCE=CATTGCCACATTCCTGTTGA
PRIMER_RIGHT_47_SEQUENCE=GTGGTCGTTTGCTGCATCTC
PRIMER_LEFT_47=918,20
PRIMER_RIGHT_47=1452,20
PRIMER_LEFT_47_TM=60.517
PRIMER_RIGHT_47_TM=61.825
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=535
PRIMER_PAIR_48_PENALTY=2.405447
PRIMER_LEFT_48_PENALTY=0.580157
PRIMER_RIGHT_48_PENALTY=1.825290
PRIMER_LEFT_48_SEQUENCE=CACATTGCCACATTCCTGTT
PRIMER_RIGHT_48_SEQUENCE=GTGGTCGTTTGCTGCATCTC
PRIMER_LEFT_48=916,20
PRIMER_RIGHT_48=1452,20
PRIMER_LEFT_48_TM=59.420
PRIMER_RIGHT_48_TM=61.825
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=537
PRIMER_PAIR_49_PENALTY=2.443920
PRIMER_LEFT_49_PENALTY=1.024212
PRIMER_RIGHT_49_PENALTY=1.419708
PRIMER_LEFT_49_SEQUENCE=TGCCACATTCCTGTTGATTT
PRIMER_RIGHT_49_SEQUENCE=ATCCGCTTGCTCTCTTTCGT
PRIMER_LEFT_49=921,20
PRIMER_RIGHT_49=1645,20
PRIMER_LEFT_49_TM=58.976
PRIMER_RIGHT_49_TM=61.420
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=725
=
SEQUENCE_ID=MAGIC.175_1_13490861_13490871_wil-2_hi-0_17261_468_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGGGTGAGCTTGNTNNNANNAATNNNAGGTGGANGNAGGAGGTGGCTGAGGGNNNGGAGGAGGTTTGGGGAGATGGTCCANCCATAGCGGCGGAGGCGGTGGAGGAGNAGATTNGAGANNNNNNNNNNNNNNNNNNNAGANGATNGTTGCTNCTNGANAGCNTTTTTTTCTTNGCTCAAAGTTATTGTNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAACACNATGTNANTNAGCAGNTNTTGAGCCAANTNGNTTCCTTTNNTTGGTATGGAGATGTGAAAANACANTCNAANTTCTCTCANAACCANTCGNANTANNCGTGAGACNAAGAATATTGCAAAGTNTNTANGTGTTGGTGTTGTGNCTCCATACGCAGANCACCANGTAGCTNGTGATTNCNTTTTTNNNNNNNNNNNNNNNCANTNGAATCCCAAAGAGTCCANAAGCACTNTTAAACTNNTNTANTTCGTCGCTTAACCNATNTTGAGTTGCANCANNAANAACCAACCTNTCAAATGTNTNNATAGNCCTCTNNCAACCTCTTCACAATGCACTNNNGACTNTGGATTATANTNAAATTNNNNATTCAAGANGTAGCCANNANGCAAAGGCTNATNGAGTTGANAATCTCANNGCNTATCGATAATTTCAANTGNATCTTTGTATTTNTCTTTTTTACCATNGAAGGTTTTANNAATGGCCNCTTTAGCCTNATCCATTNCTTCATAGATGTATCNNNTAGCTGNNTTNTTNTCTCCGTCAACCATNCTTGTGCCANAAANNNTCTTGCATAATGATCNTCTTCANAGTCCTTGCTCNTATTTCCTTTNNCNATTTGGAATCTNCCCATTNTTGANAAGTCNCNNAATTCCTCAAGTTTTTTCTNTGNNTATNATACTGNCCAANTGTNTNAAAGACGTAGCAAACCNTGTTACANCCNNTCTTTGCNGATTTCCATCGCTTNTGANNTTTNCTCATCNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAANCATTCNTGANGATGCATTGTNTGATAACAGTCTTNANTTCNNNAATCTTNCCTATATCCTCAAGCATGAGNTNTATGCAATGAGCANCANAAGGCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATAAANNAAACACAAAACGAGTTAGAAGNAGCNATGAAAAAACCAAACTCNTAAAGCTAAATNTAAANGATTNNAAANNCACTAGCTTTNACATAGTTTGATGNN
PRIMER_LEFT_NUM_RETURNED=6
PRIMER_RIGHT_NUM_RETURNED=6
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=6
PRIMER_PAIR_0_PENALTY=5.724748
PRIMER_LEFT_0_PENALTY=2.008907
PRIMER_RIGHT_0_PENALTY=3.715840
PRIMER_LEFT_0_SEQUENCE=AGGAGGTTTGGGGAGATGGT
PRIMER_RIGHT_0_SEQUENCE=AGTGCATTGTGAAGAGGTTG
PRIMER_LEFT_0=626,20
PRIMER_RIGHT_0=1304,20
PRIMER_LEFT_0_TM=62.009
PRIMER_RIGHT_0_TM=56.284
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.8000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=679
PRIMER_PAIR_1_PENALTY=6.655375
PRIMER_LEFT_1_PENALTY=2.939534
PRIMER_RIGHT_1_PENALTY=3.715840
PRIMER_LEFT_1_SEQUENCE=GGAGGTTTGGGGAGATGGTC
PRIMER_RIGHT_1_SEQUENCE=AGTGCATTGTGAAGAGGTTG
PRIMER_LEFT_1=627,20
PRIMER_RIGHT_1=1304,20
PRIMER_LEFT_1_TM=62.940
PRIMER_RIGHT_1_TM=56.284
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=678
PRIMER_PAIR_2_PENALTY=6.765195
PRIMER_LEFT_2_PENALTY=3.049355
PRIMER_RIGHT_2_PENALTY=3.715840
PRIMER_LEFT_2_SEQUENCE=GAGGAGGTTTGGGGAGATGG
PRIMER_RIGHT_2_SEQUENCE=AGTGCATTGTGAAGAGGTTG
PRIMER_LEFT_2=625,20
PRIMER_RIGHT_2=1304,20
PRIMER_LEFT_2_TM=63.049
PRIMER_RIGHT_2_TM=56.284
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=680
PRIMER_PAIR_3_PENALTY=6.765195
PRIMER_LEFT_3_PENALTY=3.049355
PRIMER_RIGHT_3_PENALTY=3.715840
PRIMER_LEFT_3_SEQUENCE=GGAGGAGGTTTGGGGAGATG
PRIMER_RIGHT_3_SEQUENCE=AGTGCATTGTGAAGAGGTTG
PRIMER_LEFT_3=624,20
PRIMER_RIGHT_3=1304,20
PRIMER_LEFT_3_TM=63.049
PRIMER_RIGHT_3_TM=56.284
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=681
PRIMER_PAIR_4_PENALTY=8.446393
PRIMER_LEFT_4_PENALTY=4.730553
PRIMER_RIGHT_4_PENALTY=3.715840
PRIMER_LEFT_4_SEQUENCE=AGGAGGTTTGGGGAGATGGTC
PRIMER_RIGHT_4_SEQUENCE=AGTGCATTGTGAAGAGGTTG
PRIMER_LEFT_4=626,21
PRIMER_RIGHT_4=1304,20
PRIMER_LEFT_4_TM=63.731
PRIMER_RIGHT_4_TM=56.284
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=679
PRIMER_PAIR_5_PENALTY=8.446393
PRIMER_LEFT_5_PENALTY=4.730553
PRIMER_RIGHT_5_PENALTY=3.715840
PRIMER_LEFT_5_SEQUENCE=GAGGAGGTTTGGGGAGATGGT
PRIMER_RIGHT_5_SEQUENCE=AGTGCATTGTGAAGAGGTTG
PRIMER_LEFT_5=625,21
PRIMER_RIGHT_5=1304,20
PRIMER_LEFT_5_TM=63.731
PRIMER_RIGHT_5_TM=56.284
PRIMER_LEFT_5_GC_PERCENT=57.143
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.8000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=680
=
SEQUENCE_ID=MAGIC.175_4_1869176_1869893_oy-0_hi-0_82575_3429830_TRUE
SEQUENCE_TARGET=950,817
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAAAATAATAGTTTCATATTAAATTTTACACGTTGGTCATATAGGGGNTCCTTGCNTANTGCATTCTGNATGGCNTGTCAGCACAGTGNATCAGCATGTGATATAATTTTACNNTAAGTAGAGCATTCATCGGNCGTNAATTTTNGNAATATTCAGTGTATTTATTTTCTNTTTTTTTCNGTTTTCTATTATTNNTGTATTTCTCAATACAATNNAAATTCAAACTAACCNAAAATTCAGAAAAGATCACTATATTATATATAAAATCAATTGCCGCCNAAAAAGAAATCTGTTTTACAATGAATATAANTGAACAATTATACATATATCTAGCAAATCTATCTNTTGGCATTTNTAAANTANTAATCTCCATTGAAGAGCTGCAACTTTGTGCTTGCTAAAACTTTTTCACNTGCAACAAAATGAGTATTATGGGAGATTTAAAAATCAACATAGGCATANAAAAATATTCCAGAAANANNAGAGAGACAATAAGGANAACAAATTTTGAGTCATATCATATTTGTTGTGATGGGCTCCATCCCATTGATAAATACCTTAATCAACCGANNGTCAGCTGAATTNCTCATGCTAATACNTAACTATACAATANTATAATTGCCATATACTGGAAAACTAGCAGATTNNACAACANTNTAGAAAAGACACAAGCATAANAAAGAAGCNTACGCGACAATTACTACANCATCTCCATATTGAATGGTCTTCTGATATACAATACCATTCCATGCGCGCAGACTGAGACTTTGTAAGTATGTGAAGCATNTCACGAGAGTTCTCAGTCAAATGCAACTCAACAAAGAAATCATAAGAAAGNATNAAATATTGAAAGNATTGAGAAGCAAATTAGTTCATGAACCACATCACATNAGCATCCTGNAGACCAAAAAAGGAAGACAATGAATCACTCAATAGAAGAACTGATAAGNTNTTGCTTTCACTGCCAAGAGTGTAANAAACATACCGCAAAAACAGACTACTAAACCCNTTTCAAGAACAATCCGAAGGAAATTGATTCTTCTCCACATCATTTAGCATCCAAAGCAATGACCACCATCTTTAGTTGCAGCTCTACGCAGCACATCTAAAGCTTTNTTGTTCTTTTACCAATTCTAAAAATCTCAAAGAAGAGATCGATTCCAGATACAANAGCTGCANNNNNNTCATTNANAGTTGTCAAAATTACAGTACCATTTTTTTACGGAGGAANGACAAGTTATGAGAGTGCTTTTNTTATATCTTTTNGGGTAAACATTGTANCGTAGTGANAGTTGAGAGTGAATTGCCTAACAACCGATAGAATCAACCAAAAANAAAAAAAGAACATCTTTTCTAACGACCNNAAAAAANNCCTAGGGGTTTTNTATTTNTTAGGTGTATATTGTCTTTTCTAGTACTAAAACAATANNNNNNNNNNNNNATCCTCACACGNANNAGATTAANNTTGAGAATATATTGTATTGTTGTTAGCATAANCTANAANGGATNAAACCAATTTCTACCAAATTCNTTTTTNTTTAGCCATTTAGANTACANTGNAAAACAAGTANTTCAANTAAAGTACTCTAAGACAATTCTTCTTCTATCTTTTATGAGCTGAAAATTTTGGACCACTTTTCTTTTNTGTTCTTTGTGGATAATAAAACCAAACTTCACNNNNNNNNNNNNNNNNNNNNNNNNNCACTGAANTTTTAGATTGATCTCAANATAAAGTTTTTTTCCTAAATATTGTCATTTATTGAATACAATTAATTAANTGTAAATTGATNNNNNNNNNNNNNNNNNNNNNTAAGACCACCATCGCTCCCTCNTCTTTCNTCAGTTAANATAATATAAANTCANTAATTAAGAGAGAAGAAATAAACANAAAAATGTTGGTTAAAGTTTTCTCTTTTTTCATCCTTATGATAACAANNNTGGTTATTGGNGTTAGCAAAGAATATTGTGATGACAAACANTCATGTCAAAACTTTTTGTTGGAGCTTAAGACAGCTNCNTCGTNACTCTCGGAGATTAGACGTNGTNACTTATTGNTCATCGTTTTGAAGAATTCCGTNNNGAAGATNGACATGGCTATGATTNGTGTGATGGAAGATACAAAACAACATGAGGAGATGGAGAATGATAANNTATGTTTNAAGGAGGACACAAATCTATTTGAGGAGATGATGGAGNCGNCAAAAGATANGATNATCCGTTNAGTGGAGGAACTCTNANGAGGAGAATTCCCTNATCTTGGATCTTACGAAAACATCCACACTTGGCTCAGTGGCGTTCTTACTAGCTACATCACATGTATCGACGAAATTGGTGACGGTGCCTATAAACGTCGGGTCGAGCCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_8344004_8344211_zu-0_mt-0_982150_148073_TRUE
SEQUENCE_TARGET=950,307
SEQUENCE_TEMPLATE=GCTCTGTTGTAATTTGTGATTGTGTTTACTGTTGCAGCTGAGCTNAAGAAGAAGAGCACAAAGCCAAAGCGGCCATCAATTAAATCCCTCTTTGGTACTTATCTAAAAGACATTNATCACAATCTATTTCAACTCATTGTCTGATATTAGCATATGGTTTTAGCACTAACAAGAATTGTTATAAAAACATTTGGCAGAGGATGAATCAGCCAGTAGCACCACAAGCCACCAAGGAAGCTTTATGAAACTCTTGCCAACACAAATCGAAGTTCCAGANAAAGAAGACAAATCAAGCTCATCTAGTGAAACCCTATCATCTTCCACCACACGTTTCGACAATGCCACATCCTAAACCAGACTCTTTGCTACANTTTGGTNCCGGTTAACACACCAAAACACGGTTTGAGGTGGCNGATTTACGGTTGGANATAGTACAGTTTCCATTGTGGCGGTGAAGGCTATTGTGTGAGAAGATTAAGGATTTGTTCAACAGTTTCTACATAGGATAATATTTGGTTGTTCAAAAGGTTTAGTTTGAAGCAAAAGGAAGTAAATAGTCATTTTCAGAATNTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_5407246_5407391_ws-0_can-0_41414_107990_TRUE
SEQUENCE_TARGET=950,245
SEQUENCE_TEMPLATE=GTGTAATTGTTATTTGGTAAATGTCNTTTTNAAAANCTTCCAANATCTAATTGTCTTCTTTCGCCGGATATTCTTTCTTACACCNNNTATTCTTTCATATGACATGCGATTGGTTTTGAAATGGGNTGNTANTGTTTCGCAACATGNNNNNNNNNNNNNNNNNNNNNTATTTTCTGAAATGGCATGCANTTGTATCTTTTGACAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_11485057_11485720_ler-0_ws-0_190993_38002_TRUE
SEQUENCE_TARGET=950,763
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNAATTTCAAATNTNATTTGTCNTATNCCGAGNGGATTNNNNNNNCAATGGCTTGTCAAAANTGNTTAAAACTTNGNAAGCAAAANAAATTTATTNTTTGCNNTTTTCNANTGATANNTANCTTACNATANAAANNCANAAGAAAAAATCNTCCAATAGGCAAACAANATAANTCTTTATTCNATGTTTTNTTTTCNATANACAATGGAGTCTNAACTCCTACACACTTNNAANCACANANCAGANAAGGAAACGTGGGTTNNGCCCTNAGAGATAGGAACATAAAGCCACTNTATTATTTGCANAANAAAACAGANATAAAAGGGAAAACGAGANNTAGTNATATGACTNAATCANTCTNNCNNNTACNTTTGGGTTGCGCCNTCTCCTNCTTCNNNNNNATCTTTAGTANNCTCANNNNNNNNNNTAANTCCTTNAAGTGTAAGNCCTCCTCCNTCTNNNATTGTTGCANNAATAGNATAATAATATNNTATAGANCCATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNAGAATCNGCAACACCAGAANTCTNGCCAANNATATCATTAAGATANATAGTCTGATTCGNANNGGTGTACTTNANCCGATATCCTNTAGNNNNATCTGCTGNATCCTCTATATTAATCTNCGNNNNNGNANCNGNNNTACTGAGAGAATTCNANNAAGAATTCGAACGCATNNNNNCNAGGTGTCGATCCCTAATTNGTATCCAAAATATGAANTCATCACGGTATAAGGAAGCAAGACACNTACTCGCTNTATTATTATTTTTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATTTACTAATATGACTCTNTGAGTTACATAGATAAGTNNNNNNNNNNNNNNNNCGNGNNTTGGTTCNNTCNNANAACATATCGAGNGGNTCAANAAAATNNCTTTGGNAAANNGNNACTCTTNTTTTGGCGTNAAGCTGGTAANNNNNNNNNNNNACGCCCATNTATGNTNACATTTCCTCCCGGTTTTCATTAATNNNNNNNNNNNNNNNNNNNNNNNNCNNNTTCNCTCNNNANAANNNGTGGAGAGCAANNNNNNNNNNNNNNNNTTTGNNNNNNNNNCCNCGATNTTTNNNNNNNNTNCGTCCNGNTNATTNNNAAACAAGNCGNCGAANNAGCTTNCCTTNCCAANGAACGGTCATNCCANAACCAAACCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTGGTGGAGCTTGG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_15744822_15745067_no-0_edi-0_93443_13523_TRUE
SEQUENCE_TARGET=950,345
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_16593234_16593298_ler-0_ws-0_54137_211051_TRUE
SEQUENCE_TARGET=950,164
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTTNAACACTTNNATTGNAAANATTAAACAGNTGAATCTTTTATTCAACATGNCCATTGTACATANTCTTAGCCTNTCTACGATNGTTACCCATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTAAACAGNTGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13894739_13894752_sf-2_oy-0_278390_79450_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_4316712_4317214_ws-0_wil-2_107728_11541_TRUE
SEQUENCE_TARGET=950,602
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCCTAGTTGTGGGNTNGGAGTCTAATGAACTTTTTCTACAANTATTACATGAATTTAAAAGTAATATTTNTATAAAATTCANAAAGAGAAATGATTNNGTTTNCTACAGAAANAGATTTNGTACANCCTTTNGAANNTTTTCTTTAGTTNAGAATNGAAGTTTTGTGTAGATTTTGAAAACAAAAAGCNGTTNTTAGNGTTAAGAGNTTNTGGTTTAANAGCATCATTCTTGAGGGTTCCTNAGGTTGANTCTTAGGATAATTGAGTTAGAAATTTAATTNTTTTAATGANAATTTGNTAAGAGCGTCTCTAATATAAGAGACAATTAATNAGCTAAGAGCGGGATAGGTGTTNAAATTAGAGTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAACCCAATTTCTCACCGATTTCTCNCCGACTTNNTCGAAACCACAGAGTAAAACCNAATTNNCGAATCATCTNCAATCTGAAANTAAACTANTNNGCTNGAAAATTNTGNNAGNTGGGACTATNANGTTCCGTNGAGCTAGACCAATTCATCACAAGCGAGAAAGAAGGAGAANNANAGGAAATAGGAGAAGGGNTTTTTNGAAGCGATTNAGGCATTAGAGAGGATGANTAGAGAACCATCAATNTACGTTTAGTTTNAAAATCGTTAANGNTGTAGNAATCTCGANTGAAANNATTATTANTGTTTGACATAATATAAGAATTATACCCATAANTNTATATATACCTATAAAACTGCAAAGAANCANTTTTTCTAAATGATGGAAAANCNTGAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13212458_13212686_hi-0_po-0_8338_55890_TRUE
SEQUENCE_TARGET=950,328
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_15563815_15564108_bur-0_sf-2_33021_1429103_TRUE
SEQUENCE_TARGET=950,393
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_17025092_17025102_ws-0_tsu-0_282575_2962_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_6119115_6119116_po-0_ler-0_425201_3087_TRUE
SEQUENCE_TARGET=950,101
SEQUENCE_TEMPLATE=CTTTGCCATTATTTACTCTCATTCGATTTTAATANAAAATTATTTAAGTTGTTGTTGTAAACAGACNNNNNNGTTGCTGTTGAGTTTGATTTAGAATAGTTAAGTTTGGTTTTGGTTGGAGAAAAGTTTTCGTTGTTAAGCCNACTCGTGATCATTTAATCTGTTCTTTTTTTGGTAGGGGGTGATCATTGAATCTTTTGNTTTGAGTTTTGTATGTCTATATANAGTCTAATACCATTAAAACAAAAAAANTGTCGNGGTNANCAGAATTAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGATGNTTTATNTNGATNATAGCGTGAGAGATNCGTTTATGGCATCTTTTGATATAATCTTCGCTTTGTAGGTCCACTTTTGATCTGATCTGATCTATTATCTATATGGAATGTTGTGTATATAATAAACTGCAGTGTAGTATTTTCTTAATAATGTGATATTAATATCTAAATTCTAGGCTGGAGAAAATGACGTGTGCTGCATATCCCCNAGACTCGAAATAACGATGTTATCGTGGGAGATGATCGTCANTTTTTTTTTTNTTNAGAGATCNNNNNNNNGTTGTGATCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATGTTTATCTTGGTCAAGAGAACGTGCACGAAAGTAGNGTAACTAATTTTACTATTTTAGATTGAGCGGTGAGACAAAAANGGGGTTAGACTCACGCGCTACACTTAGCGCGTCAGCCGACAAAANAAAGTGTTGTCATTGAATAAAAANATAAATTAAAACTGCCGCTTCGCATCTCGTGAATCATTCACGTTAAAATAGCGCACGTGCCTCGCCACGCGTCTCACCACNAAAAGAAACGCCACACACTGCACGACGATGCACGTGCCTTTTTNACCGACTATATCCGGTGACGTAACCGGGACAACCTGTAGCTGGTTTTTGCGCCAATAGGATATGAGAAAAAAGAATCNTTTTTTTCTTGTTATAGTTATAAATTAATGGTACGATTCACATGTCGCATGCGATTTGTTAATAAAACTAGAAATTGCGGTGAATNAACTTCATTCCACGTTTTTTCATTTTCTCCCTCAAAAGTCTCTGTTTNTTTNCCTTTTNCCGGCGAAGCTCTATTTAGCTTGNTNCCGGCGTTTAACACGCGTTTTAATCGAAACAGACATTTGAGATCGAATTANTTTTGTAGCGGGCTGTGTCTTTATTATAGATGGAGAGAGATTTTCTCGGGTTGGGTTCGAAAAATTCTCCGATCACTGTCAAGGAGGAAACCAGCGAAAGCTCTAGAGATTCAGGTTATTTATTNCTCTTCTCAATTTTTCTGATTCTGATTGTTTTNAAATNGTAGATTNGTTTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.840044
PRIMER_LEFT_0_PENALTY=0.707326
PRIMER_RIGHT_0_PENALTY=1.132718
PRIMER_LEFT_0_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_0_SEQUENCE=TTTTGTCTCACCGCTCAATCT
PRIMER_LEFT_0=514,20
PRIMER_RIGHT_0=1327,21
PRIMER_LEFT_0_TM=59.293
PRIMER_RIGHT_0_TM=59.867
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=814
PRIMER_PAIR_1_PENALTY=1.857226
PRIMER_LEFT_1_PENALTY=0.707326
PRIMER_RIGHT_1_PENALTY=1.149900
PRIMER_LEFT_1_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_1_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_1=514,20
PRIMER_RIGHT_1=1327,20
PRIMER_LEFT_1_TM=59.293
PRIMER_RIGHT_1_TM=58.850
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=814
PRIMER_PAIR_2_PENALTY=1.857226
PRIMER_LEFT_2_PENALTY=0.707326
PRIMER_RIGHT_2_PENALTY=1.149900
PRIMER_LEFT_2_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_2_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_2=511,20
PRIMER_RIGHT_2=1327,20
PRIMER_LEFT_2_TM=59.293
PRIMER_RIGHT_2_TM=58.850
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=817
PRIMER_PAIR_3_PENALTY=1.881203
PRIMER_LEFT_3_PENALTY=0.707326
PRIMER_RIGHT_3_PENALTY=1.173876
PRIMER_LEFT_3_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_3_SEQUENCE=ATGATTCACGAGATGCGAAG
PRIMER_LEFT_3=514,20
PRIMER_RIGHT_3=1435,20
PRIMER_LEFT_3_TM=59.293
PRIMER_RIGHT_3_TM=58.826
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=922
PRIMER_PAIR_4_PENALTY=1.881203
PRIMER_LEFT_4_PENALTY=0.707326
PRIMER_RIGHT_4_PENALTY=1.173876
PRIMER_LEFT_4_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_4_SEQUENCE=ATGATTCACGAGATGCGAAG
PRIMER_LEFT_4=511,20
PRIMER_RIGHT_4=1435,20
PRIMER_LEFT_4_TM=59.293
PRIMER_RIGHT_4_TM=58.826
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=925
PRIMER_PAIR_5_PENALTY=1.882087
PRIMER_LEFT_5_PENALTY=0.707326
PRIMER_RIGHT_5_PENALTY=1.174761
PRIMER_LEFT_5_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_5_SEQUENCE=CAGTGTGTGGCGTTTCTTTT
PRIMER_LEFT_5=514,20
PRIMER_RIGHT_5=1499,20
PRIMER_LEFT_5_TM=59.293
PRIMER_RIGHT_5_TM=58.825
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=986
PRIMER_PAIR_6_PENALTY=1.882087
PRIMER_LEFT_6_PENALTY=0.707326
PRIMER_RIGHT_6_PENALTY=1.174761
PRIMER_LEFT_6_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_6_SEQUENCE=CAGTGTGTGGCGTTTCTTTT
PRIMER_LEFT_6=511,20
PRIMER_RIGHT_6=1499,20
PRIMER_LEFT_6_TM=59.293
PRIMER_RIGHT_6_TM=58.825
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=989
PRIMER_PAIR_7_PENALTY=1.935643
PRIMER_LEFT_7_PENALTY=0.707326
PRIMER_RIGHT_7_PENALTY=1.228317
PRIMER_LEFT_7_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_7_SEQUENCE=AATGATTCACGAGATGCGAAG
PRIMER_LEFT_7=514,20
PRIMER_RIGHT_7=1436,21
PRIMER_LEFT_7_TM=59.293
PRIMER_RIGHT_7_TM=60.228
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=923
PRIMER_PAIR_8_PENALTY=1.935643
PRIMER_LEFT_8_PENALTY=0.707326
PRIMER_RIGHT_8_PENALTY=1.228317
PRIMER_LEFT_8_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_8_SEQUENCE=AATGATTCACGAGATGCGAAG
PRIMER_LEFT_8=511,20
PRIMER_RIGHT_8=1436,21
PRIMER_LEFT_8_TM=59.293
PRIMER_RIGHT_8_TM=60.228
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=8.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=926
PRIMER_PAIR_9_PENALTY=1.949409
PRIMER_LEFT_9_PENALTY=0.707326
PRIMER_RIGHT_9_PENALTY=1.242083
PRIMER_LEFT_9_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_9_SEQUENCE=TTTTTGTCTCACCGCTCAATC
PRIMER_LEFT_9=514,20
PRIMER_RIGHT_9=1328,21
PRIMER_LEFT_9_TM=59.293
PRIMER_RIGHT_9_TM=60.242
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=815
PRIMER_PAIR_10_PENALTY=1.949409
PRIMER_LEFT_10_PENALTY=0.707326
PRIMER_RIGHT_10_PENALTY=1.242083
PRIMER_LEFT_10_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_10_SEQUENCE=TTTTTGTCTCACCGCTCAATC
PRIMER_LEFT_10=511,20
PRIMER_RIGHT_10=1328,21
PRIMER_LEFT_10_TM=59.293
PRIMER_RIGHT_10_TM=60.242
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=818
PRIMER_PAIR_11_PENALTY=1.982663
PRIMER_LEFT_11_PENALTY=0.707326
PRIMER_RIGHT_11_PENALTY=1.275336
PRIMER_LEFT_11_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_11_SEQUENCE=GCAGTGTGTGGCGTTTCTTT
PRIMER_LEFT_11=514,20
PRIMER_RIGHT_11=1500,20
PRIMER_LEFT_11_TM=59.293
PRIMER_RIGHT_11_TM=61.275
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=987
PRIMER_PAIR_12_PENALTY=1.982663
PRIMER_LEFT_12_PENALTY=0.707326
PRIMER_RIGHT_12_PENALTY=1.275336
PRIMER_LEFT_12_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_12_SEQUENCE=GCAGTGTGTGGCGTTTCTTT
PRIMER_LEFT_12=511,20
PRIMER_RIGHT_12=1500,20
PRIMER_LEFT_12_TM=59.293
PRIMER_RIGHT_12_TM=61.275
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=990
PRIMER_PAIR_13_PENALTY=2.183114
PRIMER_LEFT_13_PENALTY=1.050396
PRIMER_RIGHT_13_PENALTY=1.132718
PRIMER_LEFT_13_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_13_SEQUENCE=TTTTGTCTCACCGCTCAATCT
PRIMER_LEFT_13=557,20
PRIMER_RIGHT_13=1327,21
PRIMER_LEFT_13_TM=58.950
PRIMER_RIGHT_13_TM=59.867
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=771
PRIMER_PAIR_14_PENALTY=2.200296
PRIMER_LEFT_14_PENALTY=1.050396
PRIMER_RIGHT_14_PENALTY=1.149900
PRIMER_LEFT_14_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_14_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_14=557,20
PRIMER_RIGHT_14=1327,20
PRIMER_LEFT_14_TM=58.950
PRIMER_RIGHT_14_TM=58.850
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=771
PRIMER_PAIR_15_PENALTY=2.224273
PRIMER_LEFT_15_PENALTY=1.050396
PRIMER_RIGHT_15_PENALTY=1.173876
PRIMER_LEFT_15_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_15_SEQUENCE=ATGATTCACGAGATGCGAAG
PRIMER_LEFT_15=557,20
PRIMER_RIGHT_15=1435,20
PRIMER_LEFT_15_TM=58.950
PRIMER_RIGHT_15_TM=58.826
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=8.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=879
PRIMER_PAIR_16_PENALTY=2.225157
PRIMER_LEFT_16_PENALTY=1.050396
PRIMER_RIGHT_16_PENALTY=1.174761
PRIMER_LEFT_16_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_16_SEQUENCE=CAGTGTGTGGCGTTTCTTTT
PRIMER_LEFT_16=557,20
PRIMER_RIGHT_16=1499,20
PRIMER_LEFT_16_TM=58.950
PRIMER_RIGHT_16_TM=58.825
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=943
PRIMER_PAIR_17_PENALTY=2.264540
PRIMER_LEFT_17_PENALTY=0.707326
PRIMER_RIGHT_17_PENALTY=1.557214
PRIMER_LEFT_17_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_17_SEQUENCE=TTTGTCTCACCGCTCAATCT
PRIMER_LEFT_17=514,20
PRIMER_RIGHT_17=1326,20
PRIMER_LEFT_17_TM=59.293
PRIMER_RIGHT_17_TM=58.443
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=813
PRIMER_PAIR_18_PENALTY=2.278713
PRIMER_LEFT_18_PENALTY=1.050396
PRIMER_RIGHT_18_PENALTY=1.228317
PRIMER_LEFT_18_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_18_SEQUENCE=AATGATTCACGAGATGCGAAG
PRIMER_LEFT_18=557,20
PRIMER_RIGHT_18=1436,21
PRIMER_LEFT_18_TM=58.950
PRIMER_RIGHT_18_TM=60.228
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=8.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=880
PRIMER_PAIR_19_PENALTY=2.292479
PRIMER_LEFT_19_PENALTY=1.050396
PRIMER_RIGHT_19_PENALTY=1.242083
PRIMER_LEFT_19_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_19_SEQUENCE=TTTTTGTCTCACCGCTCAATC
PRIMER_LEFT_19=557,20
PRIMER_RIGHT_19=1328,21
PRIMER_LEFT_19_TM=58.950
PRIMER_RIGHT_19_TM=60.242
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=772
PRIMER_PAIR_20_PENALTY=2.325733
PRIMER_LEFT_20_PENALTY=1.050396
PRIMER_RIGHT_20_PENALTY=1.275336
PRIMER_LEFT_20_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_20_SEQUENCE=GCAGTGTGTGGCGTTTCTTT
PRIMER_LEFT_20=557,20
PRIMER_RIGHT_20=1500,20
PRIMER_LEFT_20_TM=58.950
PRIMER_RIGHT_20_TM=61.275
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=944
PRIMER_PAIR_21_PENALTY=2.339953
PRIMER_LEFT_21_PENALTY=1.207235
PRIMER_RIGHT_21_PENALTY=1.132718
PRIMER_LEFT_21_SEQUENCE=CGCTTTGTAGGTCCACTTTTG
PRIMER_RIGHT_21_SEQUENCE=TTTTGTCTCACCGCTCAATCT
PRIMER_LEFT_21=393,21
PRIMER_RIGHT_21=1327,21
PRIMER_LEFT_21_TM=59.793
PRIMER_RIGHT_21_TM=59.867
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=6.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=935
PRIMER_PAIR_22_PENALTY=2.343245
PRIMER_LEFT_22_PENALTY=0.707326
PRIMER_RIGHT_22_PENALTY=1.635918
PRIMER_LEFT_22_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_22_SEQUENCE=GCTAAGTGTAGCGCGTGAGT
PRIMER_LEFT_22=514,20
PRIMER_RIGHT_22=1357,20
PRIMER_LEFT_22_TM=59.293
PRIMER_RIGHT_22_TM=58.364
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=844
PRIMER_PAIR_23_PENALTY=2.343245
PRIMER_LEFT_23_PENALTY=0.707326
PRIMER_RIGHT_23_PENALTY=1.635918
PRIMER_LEFT_23_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_23_SEQUENCE=GCTAAGTGTAGCGCGTGAGT
PRIMER_LEFT_23=511,20
PRIMER_RIGHT_23=1357,20
PRIMER_LEFT_23_TM=59.293
PRIMER_RIGHT_23_TM=58.364
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=847
PRIMER_PAIR_24_PENALTY=2.357135
PRIMER_LEFT_24_PENALTY=1.207235
PRIMER_RIGHT_24_PENALTY=1.149900
PRIMER_LEFT_24_SEQUENCE=CGCTTTGTAGGTCCACTTTTG
PRIMER_RIGHT_24_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_24=393,21
PRIMER_RIGHT_24=1327,20
PRIMER_LEFT_24_TM=59.793
PRIMER_RIGHT_24_TM=58.850
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=935
PRIMER_PAIR_25_PENALTY=2.449318
PRIMER_LEFT_25_PENALTY=1.207235
PRIMER_RIGHT_25_PENALTY=1.242083
PRIMER_LEFT_25_SEQUENCE=CGCTTTGTAGGTCCACTTTTG
PRIMER_RIGHT_25_SEQUENCE=TTTTTGTCTCACCGCTCAATC
PRIMER_LEFT_25=393,21
PRIMER_RIGHT_25=1328,21
PRIMER_LEFT_25_TM=59.793
PRIMER_RIGHT_25_TM=60.242
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=936
PRIMER_PAIR_26_PENALTY=2.607610
PRIMER_LEFT_26_PENALTY=1.050396
PRIMER_RIGHT_26_PENALTY=1.557214
PRIMER_LEFT_26_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_26_SEQUENCE=TTTGTCTCACCGCTCAATCT
PRIMER_LEFT_26=557,20
PRIMER_RIGHT_26=1326,20
PRIMER_LEFT_26_TM=58.950
PRIMER_RIGHT_26_TM=58.443
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=6.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=770
PRIMER_PAIR_27_PENALTY=2.686315
PRIMER_LEFT_27_PENALTY=1.050396
PRIMER_RIGHT_27_PENALTY=1.635918
PRIMER_LEFT_27_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_27_SEQUENCE=GCTAAGTGTAGCGCGTGAGT
PRIMER_LEFT_27=557,20
PRIMER_RIGHT_27=1357,20
PRIMER_LEFT_27_TM=58.950
PRIMER_RIGHT_27_TM=58.364
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.6000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=801
PRIMER_PAIR_28_PENALTY=2.764449
PRIMER_LEFT_28_PENALTY=1.207235
PRIMER_RIGHT_28_PENALTY=1.557214
PRIMER_LEFT_28_SEQUENCE=CGCTTTGTAGGTCCACTTTTG
PRIMER_RIGHT_28_SEQUENCE=TTTGTCTCACCGCTCAATCT
PRIMER_LEFT_28=393,21
PRIMER_RIGHT_28=1326,20
PRIMER_LEFT_28_TM=59.793
PRIMER_RIGHT_28_TM=58.443
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=6.6000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=934
PRIMER_PAIR_29_PENALTY=2.824607
PRIMER_LEFT_29_PENALTY=0.707326
PRIMER_RIGHT_29_PENALTY=2.117281
PRIMER_LEFT_29_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_29_SEQUENCE=TGTCTCACCGCTCAATCTAAAA
PRIMER_LEFT_29=514,20
PRIMER_RIGHT_29=1324,22
PRIMER_LEFT_29_TM=59.293
PRIMER_RIGHT_29_TM=59.883
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=811
PRIMER_PAIR_30_PENALTY=2.824607
PRIMER_LEFT_30_PENALTY=0.707326
PRIMER_RIGHT_30_PENALTY=2.117281
PRIMER_LEFT_30_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_30_SEQUENCE=TGTCTCACCGCTCAATCTAAAA
PRIMER_LEFT_30=511,20
PRIMER_RIGHT_30=1324,22
PRIMER_LEFT_30_TM=59.293
PRIMER_RIGHT_30_TM=59.883
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=814
PRIMER_PAIR_31_PENALTY=2.843153
PRIMER_LEFT_31_PENALTY=1.207235
PRIMER_RIGHT_31_PENALTY=1.635918
PRIMER_LEFT_31_SEQUENCE=CGCTTTGTAGGTCCACTTTTG
PRIMER_RIGHT_31_SEQUENCE=GCTAAGTGTAGCGCGTGAGT
PRIMER_LEFT_31=393,21
PRIMER_RIGHT_31=1357,20
PRIMER_LEFT_31_TM=59.793
PRIMER_RIGHT_31_TM=58.364
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=965
PRIMER_PAIR_32_PENALTY=3.042490
PRIMER_LEFT_32_PENALTY=1.868613
PRIMER_RIGHT_32_PENALTY=1.173876
PRIMER_LEFT_32_SEQUENCE=CGATGTTATCGTGGGAGATGA
PRIMER_RIGHT_32_SEQUENCE=ATGATTCACGAGATGCGAAG
PRIMER_LEFT_32=557,21
PRIMER_RIGHT_32=1435,20
PRIMER_LEFT_32_TM=60.869
PRIMER_RIGHT_32_TM=58.826
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=8.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=879
PRIMER_PAIR_33_PENALTY=3.043374
PRIMER_LEFT_33_PENALTY=1.868613
PRIMER_RIGHT_33_PENALTY=1.174761
PRIMER_LEFT_33_SEQUENCE=CGATGTTATCGTGGGAGATGA
PRIMER_RIGHT_33_SEQUENCE=CAGTGTGTGGCGTTTCTTTT
PRIMER_LEFT_33=557,21
PRIMER_RIGHT_33=1499,20
PRIMER_LEFT_33_TM=60.869
PRIMER_RIGHT_33_TM=58.825
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.6000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=943
PRIMER_PAIR_34_PENALTY=3.051980
PRIMER_LEFT_34_PENALTY=0.707326
PRIMER_RIGHT_34_PENALTY=2.344653
PRIMER_LEFT_34_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_34_SEQUENCE=GTGCAGTGTGTGGCGTTTCT
PRIMER_LEFT_34=514,20
PRIMER_RIGHT_34=1502,20
PRIMER_LEFT_34_TM=59.293
PRIMER_RIGHT_34_TM=62.345
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=989
PRIMER_PAIR_35_PENALTY=3.051980
PRIMER_LEFT_35_PENALTY=0.707326
PRIMER_RIGHT_35_PENALTY=2.344653
PRIMER_LEFT_35_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_35_SEQUENCE=GTGCAGTGTGTGGCGTTTCT
PRIMER_LEFT_35=511,20
PRIMER_RIGHT_35=1502,20
PRIMER_LEFT_35_TM=59.293
PRIMER_RIGHT_35_TM=62.345
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=3.096930
PRIMER_LEFT_36_PENALTY=1.868613
PRIMER_RIGHT_36_PENALTY=1.228317
PRIMER_LEFT_36_SEQUENCE=CGATGTTATCGTGGGAGATGA
PRIMER_RIGHT_36_SEQUENCE=AATGATTCACGAGATGCGAAG
PRIMER_LEFT_36=557,21
PRIMER_RIGHT_36=1436,21
PRIMER_LEFT_36_TM=60.869
PRIMER_RIGHT_36_TM=60.228
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=8.7000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=880
PRIMER_PAIR_37_PENALTY=3.138966
PRIMER_LEFT_37_PENALTY=2.006249
PRIMER_RIGHT_37_PENALTY=1.132718
PRIMER_LEFT_37_SEQUENCE=TCTTCGCTTTGTAGGTCCACT
PRIMER_RIGHT_37_SEQUENCE=TTTTGTCTCACCGCTCAATCT
PRIMER_LEFT_37=389,21
PRIMER_RIGHT_37=1327,21
PRIMER_LEFT_37_TM=58.994
PRIMER_RIGHT_37_TM=59.867
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=939
PRIMER_PAIR_38_PENALTY=3.143949
PRIMER_LEFT_38_PENALTY=1.868613
PRIMER_RIGHT_38_PENALTY=1.275336
PRIMER_LEFT_38_SEQUENCE=CGATGTTATCGTGGGAGATGA
PRIMER_RIGHT_38_SEQUENCE=GCAGTGTGTGGCGTTTCTTT
PRIMER_LEFT_38=557,21
PRIMER_RIGHT_38=1500,20
PRIMER_LEFT_38_TM=60.869
PRIMER_RIGHT_38_TM=61.275
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=944
PRIMER_PAIR_39_PENALTY=3.156148
PRIMER_LEFT_39_PENALTY=2.006249
PRIMER_RIGHT_39_PENALTY=1.149900
PRIMER_LEFT_39_SEQUENCE=TCTTCGCTTTGTAGGTCCACT
PRIMER_RIGHT_39_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_39=389,21
PRIMER_RIGHT_39=1327,20
PRIMER_LEFT_39_TM=58.994
PRIMER_RIGHT_39_TM=58.850
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=939
PRIMER_PAIR_40_PENALTY=3.167677
PRIMER_LEFT_40_PENALTY=1.050396
PRIMER_RIGHT_40_PENALTY=2.117281
PRIMER_LEFT_40_SEQUENCE=CGATGTTATCGTGGGAGATG
PRIMER_RIGHT_40_SEQUENCE=TGTCTCACCGCTCAATCTAAAA
PRIMER_LEFT_40=557,20
PRIMER_RIGHT_40=1324,22
PRIMER_LEFT_40_TM=58.950
PRIMER_RIGHT_40_TM=59.883
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.6000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=768
PRIMER_PAIR_41_PENALTY=3.192329
PRIMER_LEFT_41_PENALTY=0.707326
PRIMER_RIGHT_41_PENALTY=2.485003
PRIMER_LEFT_41_SEQUENCE=GGAGAAAATGACGTGTGCTG
PRIMER_RIGHT_41_SEQUENCE=TTGTCTCACCGCTCAATCTAA
PRIMER_LEFT_41=514,20
PRIMER_RIGHT_41=1325,21
PRIMER_LEFT_41_TM=59.293
PRIMER_RIGHT_41_TM=58.515
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.0000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=812
PRIMER_PAIR_42_PENALTY=3.192329
PRIMER_LEFT_42_PENALTY=0.707326
PRIMER_RIGHT_42_PENALTY=2.485003
PRIMER_LEFT_42_SEQUENCE=GCTGGAGAAAATGACGTGTG
PRIMER_RIGHT_42_SEQUENCE=TTGTCTCACCGCTCAATCTAA
PRIMER_LEFT_42=511,20
PRIMER_RIGHT_42=1325,21
PRIMER_LEFT_42_TM=59.293
PRIMER_RIGHT_42_TM=58.515
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.0000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=815
PRIMER_PAIR_43_PENALTY=3.239803
PRIMER_LEFT_43_PENALTY=2.107085
PRIMER_RIGHT_43_PENALTY=1.132718
PRIMER_LEFT_43_SEQUENCE=TCGCTTTGTAGGTCCACTTTT
PRIMER_RIGHT_43_SEQUENCE=TTTTGTCTCACCGCTCAATCT
PRIMER_LEFT_43=392,21
PRIMER_RIGHT_43=1327,21
PRIMER_LEFT_43_TM=58.893
PRIMER_RIGHT_43_TM=59.867
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=936
PRIMER_PAIR_44_PENALTY=3.239803
PRIMER_LEFT_44_PENALTY=2.107085
PRIMER_RIGHT_44_PENALTY=1.132718
PRIMER_LEFT_44_SEQUENCE=TTCGCTTTGTAGGTCCACTTT
PRIMER_RIGHT_44_SEQUENCE=TTTTGTCTCACCGCTCAATCT
PRIMER_LEFT_44=391,21
PRIMER_RIGHT_44=1327,21
PRIMER_LEFT_44_TM=58.893
PRIMER_RIGHT_44_TM=59.867
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=937
PRIMER_PAIR_45_PENALTY=3.248331
PRIMER_LEFT_45_PENALTY=2.006249
PRIMER_RIGHT_45_PENALTY=1.242083
PRIMER_LEFT_45_SEQUENCE=TCTTCGCTTTGTAGGTCCACT
PRIMER_RIGHT_45_SEQUENCE=TTTTTGTCTCACCGCTCAATC
PRIMER_LEFT_45=389,21
PRIMER_RIGHT_45=1328,21
PRIMER_LEFT_45_TM=58.994
PRIMER_RIGHT_45_TM=60.242
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=940
PRIMER_PAIR_46_PENALTY=3.256985
PRIMER_LEFT_46_PENALTY=2.107085
PRIMER_RIGHT_46_PENALTY=1.149900
PRIMER_LEFT_46_SEQUENCE=TCGCTTTGTAGGTCCACTTTT
PRIMER_RIGHT_46_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_46=392,21
PRIMER_RIGHT_46=1327,20
PRIMER_LEFT_46_TM=58.893
PRIMER_RIGHT_46_TM=58.850
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=936
PRIMER_PAIR_47_PENALTY=3.256985
PRIMER_LEFT_47_PENALTY=2.107085
PRIMER_RIGHT_47_PENALTY=1.149900
PRIMER_LEFT_47_SEQUENCE=TTCGCTTTGTAGGTCCACTTT
PRIMER_RIGHT_47_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_47=391,21
PRIMER_RIGHT_47=1327,20
PRIMER_LEFT_47_TM=58.893
PRIMER_RIGHT_47_TM=58.850
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=937
PRIMER_PAIR_48_PENALTY=3.294935
PRIMER_LEFT_48_PENALTY=2.162217
PRIMER_RIGHT_48_PENALTY=1.132718
PRIMER_LEFT_48_SEQUENCE=TTCGCTTTGTAGGTCCACTTTT
PRIMER_RIGHT_48_SEQUENCE=TTTTGTCTCACCGCTCAATCT
PRIMER_LEFT_48=391,22
PRIMER_RIGHT_48=1327,21
PRIMER_LEFT_48_TM=60.162
PRIMER_RIGHT_48_TM=59.867
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=6.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=937
PRIMER_PAIR_49_PENALTY=3.312117
PRIMER_LEFT_49_PENALTY=2.162217
PRIMER_RIGHT_49_PENALTY=1.149900
PRIMER_LEFT_49_SEQUENCE=TTCGCTTTGTAGGTCCACTTTT
PRIMER_RIGHT_49_SEQUENCE=TTTTGTCTCACCGCTCAATC
PRIMER_LEFT_49=391,22
PRIMER_RIGHT_49=1327,20
PRIMER_LEFT_49_TM=60.162
PRIMER_RIGHT_49_TM=58.850
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=937
=
SEQUENCE_ID=MAGIC.329_4_1778445_1778988_wil-2_ws-0_282240_254081_TRUE
SEQUENCE_TARGET=950,643
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTTATANGATAACGGAGANAAGTCACCTTAACTTGATCTCTTTATTGTGTTTTCAACGTTTTGTTTNATGNCCACCATATTCAATTATATAATAATTATTTNNTTTTTAGTNTCATTCTTCTNCATATACCTTTNTTTATGNNAATTTNTTTNAATATAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_12981604_12981793_wil-2_zu-0_195370_210253_TRUE
SEQUENCE_TARGET=950,289
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGATCTAATATTTCAAAGATATTCGTNANNATCTTGGAGTATAAGTTGCAAATCAAAGATGGGATAATGACTAATGATTCGNAAATATGGGATAATGANTAATGATTCGNAAATTGTTTAGTTCATAATGNTNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANTCGAATATACAGAAGATTATAAAGNAATATGCNTGTAACAATGTANATTTNGTTNNGATATAGAACTAAACNTGTTNTNCCTNAGATTTTGGTAAGATTGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_13649428_13649437_ws-0_no-0_32541_156934_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_11769949_11770412_oy-0_no-0_118879_153502_TRUE
SEQUENCE_TARGET=950,563
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_5_10064799_10064972_edi-0_tsu-0_4760_12373_TRUE
SEQUENCE_TARGET=950,273
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNGTNGTTATACNCTGAAAAAGCNGGATATCCGGCTCCGTTACAGGGNTNANCCANTTGGTTATTCATTTACAACATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGCCANNNCTCACCTANTTTTTNATTACAACACANNATTNTAGTGNAAATNTNTTTTCNCCAGTTGTNAAATNGTTTACNACAAACCATNNNNTTANTCTTANGAGAANNCACNNCAGCACTCTCCCCAAAGNTCTNANAATCTCTCTTCTAAGTGCCATGTGGCATAAGNCAAATGTCANTTCNACNTCATNTGAGATNGAANGCNTCCTCTTCTTCANGAAANTTCNTNGGAGTTGNGTTCNATTCAGGNTANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_9015836_9015842_edi-0_wil-2_4986_882_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14472179_14472372_sf-2_ws-0_16591_11733_TRUE
SEQUENCE_TARGET=950,293
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCATNGGTGTTGTNTAGATGAGAACCTATCAACACANNAGGATNAAGNACTTGGTATATTATAATAAAATTATNTGTGNTTTNNNNGAAGAGAATTATGGTCNTTTTACGAATCTTTGTAATGTTTGGTATCTACGATGGTGCATACCGCGAGTTAGCCAAATGTTATGTATTTANTNGGAAACGCGTTTCCATGACGTTTTTTGTGTTTCCAACCTCAGAGCGAATTTCCNTGCNNCCNTNNCNATCGTCNTTGGNATCTGCTCCACCGNCNATTNANATCCCATGCATTCTTGNTGGGAATCANTGTCCAAGCAATNNNNNNNNNNNNTNTCTTGCATCATTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTNTNNTTNTATNTTATTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14648862_14649099_zu-0_tsu-0_107888_24051_TRUE
SEQUENCE_TARGET=950,337
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTTTGGATTGGATNGAGNATNGATGNNNGTGGGGGACCNCAACAAAGATNTTAAAGTGTTAAAGAATCCTAGCCNAGCTCNGCAACGNGGGCTTGTAACTCAAGTGAGGNCTAGTGGGGAAGCGGGTGAGTTGTTTCAGAGAAAACTAAGGAATCGTGAAAGAGAAGTTGAGNNNNNNNNNNNNNNNNNNNTAAGAACCAAGGTGGGTGATGAGATGCGAGTTGTAGNGAACAAGAGTNGAAGGTGTAACTATCATTTTTGAGGAAGNAGGAAAACGGAAGATGAATNACATTAATTGGAAGAANGATGAGAAAGACTTATTAATCGGAGNAAGGTATGCGGGAAGTNATGAAGACGAGAAGNAGTAAGTNATTGGAAGGAGNNNNNNNNNNNNNNNNNNNNAACCGGTTTTAAACTTAAGAACCAGTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACCAATCNGATTTTGATCAATTCNNNCTNNNNTNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGGCTNTNTAGGAAGGTATAGATNAACCCCTAATTCTCCCCAAAGCTCTTTGGANGGNTGAAAACAATTCAGCACNAAACCANNAACAACANATGCCACAACCAACCAAAAGTTNCAAATTGACANTGGATGCGGCTTNTAATTNGTTGGGGCTTTAANANGCATATATATACAACATATTNAGGTTAANAAACGAAGNNNNAANTTGCCAAAAACTCNCCTTCGTTTTNGGAAAGGATCAACNCGNTTNNTACCTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_8025103_8025285_po-0_tsu-0_704311_5446_TRUE
SEQUENCE_TARGET=950,282
SEQUENCE_TEMPLATE=ATCGATGATCAATATGATGGTCTGACACATAGNCTACCATATGAGAAANACGATCTATATACATGTCTTAAATGCNACAATATATTTGATATTTCTNAAAATTTTGATGTACATATGTCATCTCACTACAAGTATTATATGGATAAAAAANGAGATTTTGAGACTTCGGACAAAAAATAAATAATNAAAAATTGTACGAGATATAATGGTGGAAANAGTGTNGATGAACCTACTCAACATTTTGAGATAGTTAAAGAAGAGATTATTTAGGATTTTCTTTCCTTGANATTTCTTTTTAACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGCATGAATCAGATTATATNNTCTCCTTGGATCCTTTGGTCGTTCNNNNNNNNNNNNCNNNANNNNTCANAAATGGATCTTTTTTGTCTTATCATACAACTTTTCACCTTCTNTTAAATATAATATATAATTTATTATTGCAAAATTGGCTTTTTTAAATCCCGAAAGTTTTGAAACCGTATCTTTCNATCTCATAAAAGAGCAGTGCTAATTAATACCCAACACNAAAAAAACTCAAATAATTTAGGTAAACTTTTGACGAAGTGTATAATTACGAGTCAATATTAACAAATGTTAGTTAATTCAGCTGACAAGTGTNTGTCGAAGTGATATGTTCTTCACGTAAAATGCCTTTTTAAAATTNTTAGGTTTTTCGCGCGGTAACTTNAAAAATTTGTTTGATAGTAGTTAAGTTAAAATATTATAGTAAGACTAAGAGTATACATTTTCTCAATACATGTGATTGGGATGGTAGGAAAATAGTTACTACTTTCAAAAACCTGATAAGACAATGGATTATCNCANATTCTTTCTTTTAAGGTAAGTTGACTATTCTGGACTTTGCTAAATGTCTTNCAAGATTTTGGACTACTCTTTTGCTTGTTGGTTGCTAGCTTCAAATGCAAGCTAACAAGAACTGTGAGTTCTTTTTGGACTAGTCTTATTATGCTTGTTGGTTGCTAGATCCAAATGCAAGCTAATTCGAGCTAAGTAGAACTTTTGTATCGGTCAAACTCGAGTTACATCAATCTTTGATGCCTANGGGTTAGGGTTTGTATAANTTTCTTAGGATTAGCTTGTCAATTTAAGTCATAAGTATATACAGNANAAAAAAATAGGTATCAACATTGACATCATACATGTGATNACGTTACAAAANTTATTTTAATTACATGTAATTTATTACAAGANTAGGCTNAACATGATTNTGNGCGATACTACGATATGTAATCANAATTNNNNNNNNNNNNNNNNNNNNTCTTTTCGATTTTTGAAAATATGTANTAACAAAAAAAATTGATCTAAATATANAGAAAGCAAGAAAGTATTTAGGAATA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4449362_4449380_tsu-0_mt-0_58616_31871_TRUE
SEQUENCE_TARGET=950,118
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTCGATATTATTCACNAAATCTTGNGTTTTACTTNGAGNTAACTCCAAATCTNATGAAAACCCTAGNACTAAGCTAAANNCTACTNTATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNACACATTTTCTTCCCACATNNNTAAATTTGAATTTATTANAGNTANNCACCANAAGCAAACNATGCCAAGANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTACATTTGATGAAGTAGCTGATCNTTTTACTTNGANCAGAAAANNNNAAAAGTTCCAACTAAGGNAAAGATCTNCCAAAATTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTNTCNNAGNTCCAAAAAATNACAAAGAGNTGAGGACGTGNGATGGTGTTGTGTATGAGACNTACACTGAAGTNTGTGCNGNNCGAGNATTTATGTAANAAGAGNGNNNNNANNCCNTNNNNNNNAANNTNTNCNATTCAATTTNCTTTAAGTTCTATNTNTTTATTTTCAATTNCTNCTTTGTTATTCANACNNNNNNNTAATTCATGTTTNANTTATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4712351_4712649_rsch-4_wu-0_68949_9861_TRUE
SEQUENCE_TARGET=950,398
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_13170254_13170901_can-0_sf-2_6896_445964_TRUE
SEQUENCE_TARGET=950,747
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTGTNAAANTANTAANNNNNNNNNNNNNNNNNNNNNNNNAGTTAGGCCGATNANTCNAACNATATAGCAAGTAANNANANTAAANNGTAATTTNTAACNNGTGAGTGANTTNGTTTGAGNAACCANAANGGNCTNAGNTTAGGGTTNGCATCTCNGGGCTCANNCTCTTCNTNNGANGATTTGNNNNNNNNNNNNNNNNNCANNCCNANATCTAGAAGAAGNNNNNNNNATGACTGGAGGAGGGGGACCCATTGATCGNGGTNGGGGAGATGATCGAGGAGAACNATCAGTTCNGGCTNTNNTCTTATCTCTCTTAGGANAGGAACTGCTGCGNGTTGNTCTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNCNGANGTGAGTCACGNNNNNNNNNNNNNNNNNNNCGAGGTGACCNGCNGCCATGACCTTNANGNTGAGGTGTNGGTCTNAANGCACNNGCAGATNTNNCCCCTTCGNCGCTACCATCNTCGACNAGNANNGTTCTACTGTANTCNTTCNTGTCGACAGCNGACTNNAGNAATGANTTGTTCNCCTCACANGCCTNTNTCATNGGCTTGGANAACNNNNNNNNNNNNGAAGCGATAGCCCTNNCAGAAGTTCCGTGAGGTANAGNTGGAGNTGGTGGAAGTTGNGTNGNNNNCCGGNTGGNCTCATTNTGNNNTTATNATTNGCCTNAANGAACCGNTCGANNGTGAAAGAGTTCNAGAGGGTCTCNCNNTCGATNACNATCTTNANGAANNNNNNNNNNNNNNNNNTNGGGAGAGTTGGAGNAATGAAAGCTNNNAANTNNAAATNATAAAATNNATTANNNNNNNNNNNNNNNNNNNCNAAAAGNCATATNTNACCTAANTTCNACCAGGTTTNNNAGACCACTTCGATGGAAGCANNNNNNNNNNNTTTCNAATCGAGGCAGGGGTTTTNTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_6860418_6860964_ws-0_oy-0_63528_210319_TRUE
SEQUENCE_TARGET=950,646
SEQUENCE_TEMPLATE=CCTCGAATGGGCCAGTGAACGCAAAGTAGTTTTAACAAGACACAATGTCGAGTTGGGCACTTGCCTGTTGTAACAGTCAAGGTAAGAATNCAGACGATATTCTNAATCCTTGNTCCTCATTCTGCTCGAGTTTTGTTTTAGAGGACTCATATCGAGTCCATGTTNTAGGTTCTCNGGAGGCTATCNAACGTTCCTTTTTTTGTGTNNTANTTGAGAACGAAATTGACTGTTCTTGTTTTGTATATGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGCCTCGACTCTAAGCGCTGCCAAACTGATTTATGGTTTTTATCCTTCTCGTAGGAATCCCTAGAGTGATAGGATGCTCGATGCTCATAGTGTTTGTCACGGGATTGGACTTTCTTAGCATAAGCTTGGTTTCTGGAGTTAACATTCCCATGAGAACTTTCATAGGAGAAGCTCTCTGATCTTTTCCTATCCCTTGGCGAGTAGTTTCCTCTCTTCCTTTCATTATGTTTGTCATCCAATGCTTTATTATACCTATCAGCCGAAACATCGGATGAGAGAGTTCNNTNTNNCNTNTNGAATTCANTCAATATNCCNNTAAATANGTGCCAACAAGATCTTAGANNATGNGTNTGATTGGTTNTCCTAGTTNNCNNNNAAAAAANNTGAAAAANAATTNTGCNCAATTNATCTNTTTTTACANCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAAAAACACNANAACAGTGTAAATTGTTNNACTNTANATTTATTTTAGTGGCTGAAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAATCACACTGCCGTTANATTATGTGNCANAGTAAAATANAATANGTNAANNNNNNNNNTATNNATNNTAAATCTTNNNAAAANNCTTAAANCACAATGAAATGNCACATTANCAACCNAAAAAAAATGGTTCCTNNTTCCTACTCTTTTAATANACTTTGATTTTTATACGTGTTTTNGTAGTTCATTCGANCTATTCTAGGTAGATTAACCAACCATATGTAAATGATCTAGTCATCCTCAAAATTAGTCAAACGCTAGTNCAATATAAATTTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAATAGACCGCCTTNACAGANGCCAAATTCTATTTTTCAGTACATNAAGACAATATTGANATTATTGTTTTGACTTTTAGACNAGGAATTACNATGCNTNTGNNNNCTACGATATTACNAATATAATTTTCTGGTCAAATGGNNAAAAANNNTATTAAAAANGGAGAGAGAGATGNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_15098294_15098500_bur-0_wu-0_23918_7175_TRUE
SEQUENCE_TARGET=950,306
SEQUENCE_TEMPLATE=CTTCCGAAATTGAAGCTNGCACCATGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAGAGGGTTTNTTCAAAACTTTGATACAAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTATGATAATTAACTCTTAAACNNNNNNGATGGAGAGATCTCNGTTTATAGANGNNGAATTTTAGNNAACACCTCTTAGAGTCTTAGGAATTGGAAANAAAATNTNNAAANNCCCNAANAGGAAAAAGGNAACNAACTAGAAAAANGNNANNCAANNAAACAGAAGGGAAAGCTAGACTTGAAGANNACACNTGTATGTTNTNTTTTTTAATTAGGGTTTTGTGAACCTTTGTNTNTTAAATATCTTNTATTGTAAANGTTGAAGANGTGTTAAAATCATANNNNNNNNNNNNNNNNNNNNNNNNNCAATCAAANAGACATNGACATGTNTAAAAATATTTTANTTTTCNTTTGACATTATTTTGTNTACGTCTTCCNATTAAACTACNNNNNNNNNNNTGNNATTTGAAANTATGTCTTATGNNNNNNNNNNNNNNNTTGAGGTGCAAGTTTCTNTTTGTNNNNTATATAAGGGAGACATAAACTTATGAGAAGTTGGNTTTTGATANAANAAAAATCCCNANGAGATTNGATGTGAGAGATTNTGGGTGATCATNTGNNANGTAGNTTCGCAAATTTGGTGNNTGTGTGATCAANCTTATTTGTGTNTTGGTNNTTACNNTTCGTGTTGGAGAGTTTTGGTGATNGTTGGNTTNGTTNGTNCGNAGAATTGAANTCTNGGTTNAGNGGGAGTTTAGNTCNGGTTAGNTTATCNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAGTTAANGGTNGAGATTTATNCTTAAGANTTNATNTTAATAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGTTTGNGAATAANTTGTATTTGTTTTNTNGNTNTANTGAAATCNAAATCTNGANGTGGTCNCGGAGAGTANAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTCTCGATAGAGATTTAGTNTTNTTTCGGGTANANNNNNNNNNNNNNNNNNNNNNNNNTNANGAGATCANTCATGAGATCATNAAGGAAGTAAAACGGANCTAAGAATCGTGNGTTATNGATTNATACAANTCANGAATACTTGGTCTAGGAGANTTAATCATTNNAGAGATGNTGCAATGGNNNNNNNNNNNNGGNTTCGANGGAGNCGATNCTTGAATTAGTTTTTGNCATNATGTGACTCAATTAAGGGGANNTTGGNGTTGCTNGGTTGATGTGTTANTGGTGATGAAGTCATNGAANNTAGAGTTGNTGAGTTCANATACATANTCAAAANNGGNNAGATTGNANATGCAATTATNTCGTTGTTCTGTACTAGTCGTCNNNNNNNNNNNNGAAGTAGTNCAAGAANTGATTCAAGAAGTTGANATGCATAAATGATNANNAGGANNTCNAGTACTTGGATTTNGGANNGTGTGTNACGTCAAGAAAAANNNAAATTATNNNNNCTNNAAGANAAANNGNNNNNNNNAAGTTTGCTTNAAGAGAAAAAGAAANTGGCANAANTGTGGCTCGAGTNNTACNNANCGGTTAGTCTTCTTAGCCAAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAATCTCGTAGATGTACATTCCANAGGAAAGNNNNNNNNNNNNNNNNNNNNATNCATCCNTGTATTAAGAAATCCNACAATTNAAACTTCTAGAGATGNCACAAGAGATTGTGTTAAANATAGACANANANACTTAGNCNATAGTTCNAATTCTACTGCTACTACNANCNNNNNNCNNTGCAAATNNNNTANATACACCAAAAANTAGAGTCTGCACAGNNTTTNAGNCTATCCACNAAAACACTNATTNTTTCTATTCAACATCCTCATCAGTAAGTAAGGTTGAGCCAATCAAACTCATTNTCTGGCAGCTCTTTCTCCATTTCTTTCACTCCTCTCTCNACAATCACATTGATTCTCCACAACACAATCTTACTTNCCCATTTCATGCCTTTGANTACATAGAGTCTCCAAAGCAGACCCTTAAC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_5792963_5793265_kn-0_po-0_15269_255030_TRUE
SEQUENCE_TARGET=950,402
SEQUENCE_TEMPLATE=TATGAAACAAAATTNNATTTGTCTTTATGTTGCTTNCATATAGAAAAGTNCAAAACTTTGTTAGAGCTCATGTAACTTCNTNNNNNNNNAAAACAAAACAAATCAGATCCAAAGTGTAATAAAGAGAAACTCTAGGGACTTCTTTATAAATAAAACAANAANCATTTACACGTCTTCGTGTAATTTCGAAGAAACATCNATTCTCTTCTCTTATATAAACCCTCTTCGTCTCCTCCTCCTCTCTGCTCGAAACAAGATCCTTCACTTCTCTCAAACTACGAAAACTATGGCTTCAAAATCCNTCGTCGTTTTNCTCTTCCTCNNTCTCATAGCTTCNTCAGCCATAGCTCAAGCTCCAGGACCAGCNCCAACAAGATCTCCTCTTCCATCTCCTGCTCAACCTCCAAGAACCGCCGCACCAACTCCTTCAATCACACCAACNCCAACTCCAACACCATCCGCTACTCCCACGGCGGCTCCNGTTTCTCCTCCGGCTGGTTCACCACTTCCCTCNTCCGCTNCACCACCCGCTCCTCCAACTTCTCTCACTCCNGATGGTGCNCCTGTCGCTGGTCCGACTGGATCNACTCCCGTCGANAACAACAACGCCGCCACTCTCGCNGCTGGATCTTTAGCCGGATTTGTATTCGTCGCTTCTTTGTTACTGTAATTTGGTTATATTTTAGAATCATCTATTGAGATAGNGTTTTTCTATTTAAGGATTTTTTTGTTCGTGTTATTTTTAATCTTTGGTTTTATGTTGTATTAAGAATTGAGTTAGCGNTTGGACGATAGAATTAAANCANTAATCNNNTGTNTTTTNTATTATAGTCTTTAGTAAACANNNNNNNGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTTATCACTAATGTTTTATTATGGTATTNNANANTNCGTATGGATTTGAATTAATTGATTTAGTGAAGCCTTGTGGAATTCGAAATGGGTGGGGTTAAGTGGTTAGTGAAATAGAAGGTGATTAGTGATGACTGATGAGGTCAGCAAACGTGTGCGTGTGTAAAGACACGTGTCGACNTATATAAGAACGGTGAATTTGTCGTTTTATTGGTAGTGGCGAAAAAACAGCTGTTTGGTTTTCTGTCTGATTCTTCATCTCTTTTTTTTACACTTTTATTTGAGCTAAACGATNNAAATGAGATTGGATAACGAAGATTGAAAAGTATGTAATATTAACGAGACTAATGGCGTTTAGAATTGAAAAATCGANTTAAAAAAGTCTTTGGTTTGGTGAGAAAGGTAAGAGTAGGTTTTGTCACGTGGAGACAGCTGTAAGAATACATATAAAGCNTTTTTCAGGATTCTTTCATTGGCTCAGCCATGTGGGCTTTCCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTAAAGGTGGCGTGACAAAATATTATACTCAATACTATAAGTTACTTAAAATTANGAAAAANTTTAATTAAATCAATGCGTTTGCTATAAAAGTTAGANGAAGAAGATATACTATCGTTTTTTTGTTAACATAAATCGAAAAGAACTCTCAAGTCTTTTTAGGTTTTTGCACAAAAGGAGGATAAAGAGAAAAAAAGGTAAAAAGAAAGTTGAAGAAATTGTTCCNTTTTAAAGGGAAAGGCTGGAGAGCATTTGCATTCNTTAGCTGTGAATTCTGATTCATTTTCATTCCCTCCTTAATATTCCAACTCAACGAAAATGAAAAGTANCCACAATTTGTCCTTTTTCATATACCAATGTCATTTATTCTTTAGCCATTAATAACCTNTAAAANGTACCACATAAAATTTTATANATAAAGAAAAAGNATGGAAGACAAAGTACAAGAAGCCTTTTTTGAATTAGTACTTTAGTACCAAATGCTCGAGGTGANATCTAACTGAACCATAGTGTTGTTGCCNCGCGAAAAGGANAANGTCTCAAGCAAGAAATCTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.432188
PRIMER_LEFT_0_PENALTY=0.003322
PRIMER_RIGHT_0_PENALTY=0.428866
PRIMER_LEFT_0_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_0_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_0=445,20
PRIMER_RIGHT_0=1438,20
PRIMER_LEFT_0_TM=59.997
PRIMER_RIGHT_0_TM=59.571
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=994
PRIMER_PAIR_1_PENALTY=0.524494
PRIMER_LEFT_1_PENALTY=0.095629
PRIMER_RIGHT_1_PENALTY=0.428866
PRIMER_LEFT_1_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_1_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_1=636,20
PRIMER_RIGHT_1=1438,20
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.571
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=803
PRIMER_PAIR_2_PENALTY=0.562606
PRIMER_LEFT_2_PENALTY=0.133741
PRIMER_RIGHT_2_PENALTY=0.428866
PRIMER_LEFT_2_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_2_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_2=446,20
PRIMER_RIGHT_2=1438,20
PRIMER_LEFT_2_TM=60.134
PRIMER_RIGHT_2_TM=59.571
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=993
PRIMER_PAIR_3_PENALTY=1.096931
PRIMER_LEFT_3_PENALTY=0.668065
PRIMER_RIGHT_3_PENALTY=0.428866
PRIMER_LEFT_3_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_3_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_3=528,20
PRIMER_RIGHT_3=1438,20
PRIMER_LEFT_3_TM=60.668
PRIMER_RIGHT_3_TM=59.571
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=911
PRIMER_PAIR_4_PENALTY=1.495435
PRIMER_LEFT_4_PENALTY=1.066570
PRIMER_RIGHT_4_PENALTY=0.428866
PRIMER_LEFT_4_SEQUENCE=TCCAACACCATCCGCTACTC
PRIMER_RIGHT_4_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_4=447,20
PRIMER_RIGHT_4=1438,20
PRIMER_LEFT_4_TM=61.067
PRIMER_RIGHT_4_TM=59.571
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=992
PRIMER_PAIR_5_PENALTY=1.598310
PRIMER_LEFT_5_PENALTY=0.003322
PRIMER_RIGHT_5_PENALTY=1.594988
PRIMER_LEFT_5_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_5_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_5=445,20
PRIMER_RIGHT_5=1438,21
PRIMER_LEFT_5_TM=59.997
PRIMER_RIGHT_5_TM=60.595
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=994
PRIMER_PAIR_6_PENALTY=1.598310
PRIMER_LEFT_6_PENALTY=0.003322
PRIMER_RIGHT_6_PENALTY=1.594988
PRIMER_LEFT_6_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_6_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_6=445,20
PRIMER_RIGHT_6=1439,21
PRIMER_LEFT_6_TM=59.997
PRIMER_RIGHT_6_TM=60.595
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=995
PRIMER_PAIR_7_PENALTY=1.690616
PRIMER_LEFT_7_PENALTY=0.095629
PRIMER_RIGHT_7_PENALTY=1.594988
PRIMER_LEFT_7_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_7_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_7=636,20
PRIMER_RIGHT_7=1438,21
PRIMER_LEFT_7_TM=60.096
PRIMER_RIGHT_7_TM=60.595
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=10.2000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=803
PRIMER_PAIR_8_PENALTY=1.690616
PRIMER_LEFT_8_PENALTY=0.095629
PRIMER_RIGHT_8_PENALTY=1.594988
PRIMER_LEFT_8_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_8_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_8=636,20
PRIMER_RIGHT_8=1439,21
PRIMER_LEFT_8_TM=60.096
PRIMER_RIGHT_8_TM=60.595
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=804
PRIMER_PAIR_9_PENALTY=1.728728
PRIMER_LEFT_9_PENALTY=0.133741
PRIMER_RIGHT_9_PENALTY=1.594988
PRIMER_LEFT_9_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_9_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_9=446,20
PRIMER_RIGHT_9=1438,21
PRIMER_LEFT_9_TM=60.134
PRIMER_RIGHT_9_TM=60.595
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=993
PRIMER_PAIR_10_PENALTY=1.728728
PRIMER_LEFT_10_PENALTY=0.133741
PRIMER_RIGHT_10_PENALTY=1.594988
PRIMER_LEFT_10_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_10_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_10=446,20
PRIMER_RIGHT_10=1439,21
PRIMER_LEFT_10_TM=60.134
PRIMER_RIGHT_10_TM=60.595
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=994
PRIMER_PAIR_11_PENALTY=1.802576
PRIMER_LEFT_11_PENALTY=0.003322
PRIMER_RIGHT_11_PENALTY=1.799253
PRIMER_LEFT_11_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_11_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_11=445,20
PRIMER_RIGHT_11=1410,21
PRIMER_LEFT_11_TM=59.997
PRIMER_RIGHT_11_TM=59.201
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=966
PRIMER_PAIR_12_PENALTY=1.830375
PRIMER_LEFT_12_PENALTY=0.031122
PRIMER_RIGHT_12_PENALTY=1.799253
PRIMER_LEFT_12_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_12_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_12=624,20
PRIMER_RIGHT_12=1410,21
PRIMER_LEFT_12_TM=60.031
PRIMER_RIGHT_12_TM=59.201
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=787
PRIMER_PAIR_13_PENALTY=1.864550
PRIMER_LEFT_13_PENALTY=1.435685
PRIMER_RIGHT_13_PENALTY=0.428866
PRIMER_LEFT_13_SEQUENCE=CCGCTCCTCCAACTTCTCTC
PRIMER_RIGHT_13_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_13=527,20
PRIMER_RIGHT_13=1438,20
PRIMER_LEFT_13_TM=61.436
PRIMER_RIGHT_13_TM=59.571
PRIMER_LEFT_13_GC_PERCENT=60.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=912
PRIMER_PAIR_14_PENALTY=1.883935
PRIMER_LEFT_14_PENALTY=0.031122
PRIMER_RIGHT_14_PENALTY=1.852814
PRIMER_LEFT_14_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_14_SEQUENCE=TATTTTGTCACGCCACCTTT
PRIMER_LEFT_14=624,20
PRIMER_RIGHT_14=1556,20
PRIMER_LEFT_14_TM=60.031
PRIMER_RIGHT_14_TM=58.147
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.2000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=933
PRIMER_PAIR_15_PENALTY=1.894882
PRIMER_LEFT_15_PENALTY=0.095629
PRIMER_RIGHT_15_PENALTY=1.799253
PRIMER_LEFT_15_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_15_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_15=636,20
PRIMER_RIGHT_15=1410,21
PRIMER_LEFT_15_TM=60.096
PRIMER_RIGHT_15_TM=59.201
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.2000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=775
PRIMER_PAIR_16_PENALTY=1.932994
PRIMER_LEFT_16_PENALTY=0.133741
PRIMER_RIGHT_16_PENALTY=1.799253
PRIMER_LEFT_16_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_16_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_16=446,20
PRIMER_RIGHT_16=1410,21
PRIMER_LEFT_16_TM=60.134
PRIMER_RIGHT_16_TM=59.201
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=2.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=965
PRIMER_PAIR_17_PENALTY=1.948442
PRIMER_LEFT_17_PENALTY=0.095629
PRIMER_RIGHT_17_PENALTY=1.852814
PRIMER_LEFT_17_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_17_SEQUENCE=TATTTTGTCACGCCACCTTT
PRIMER_LEFT_17=636,20
PRIMER_RIGHT_17=1556,20
PRIMER_LEFT_17_TM=60.096
PRIMER_RIGHT_17_TM=58.147
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=10.2000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=921
PRIMER_PAIR_18_PENALTY=2.015475
PRIMER_LEFT_18_PENALTY=0.003322
PRIMER_RIGHT_18_PENALTY=2.012153
PRIMER_LEFT_18_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_18_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_18=445,20
PRIMER_RIGHT_18=1410,22
PRIMER_LEFT_18_TM=59.997
PRIMER_RIGHT_18_TM=60.012
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=2.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=966
PRIMER_PAIR_19_PENALTY=2.015475
PRIMER_LEFT_19_PENALTY=0.003322
PRIMER_RIGHT_19_PENALTY=2.012153
PRIMER_LEFT_19_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_19_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_19=445,20
PRIMER_RIGHT_19=1411,22
PRIMER_LEFT_19_TM=59.997
PRIMER_RIGHT_19_TM=60.012
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=2.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=967
PRIMER_PAIR_20_PENALTY=2.043275
PRIMER_LEFT_20_PENALTY=0.031122
PRIMER_RIGHT_20_PENALTY=2.012153
PRIMER_LEFT_20_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_20_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_20=624,20
PRIMER_RIGHT_20=1410,22
PRIMER_LEFT_20_TM=60.031
PRIMER_RIGHT_20_TM=60.012
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=787
PRIMER_PAIR_21_PENALTY=2.043275
PRIMER_LEFT_21_PENALTY=0.031122
PRIMER_RIGHT_21_PENALTY=2.012153
PRIMER_LEFT_21_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_21_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_21=624,20
PRIMER_RIGHT_21=1411,22
PRIMER_LEFT_21_TM=60.031
PRIMER_RIGHT_21_TM=60.012
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.2000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=788
PRIMER_PAIR_22_PENALTY=2.107782
PRIMER_LEFT_22_PENALTY=0.095629
PRIMER_RIGHT_22_PENALTY=2.012153
PRIMER_LEFT_22_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_22_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_22=636,20
PRIMER_RIGHT_22=1410,22
PRIMER_LEFT_22_TM=60.096
PRIMER_RIGHT_22_TM=60.012
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=10.2000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=775
PRIMER_PAIR_23_PENALTY=2.107782
PRIMER_LEFT_23_PENALTY=0.095629
PRIMER_RIGHT_23_PENALTY=2.012153
PRIMER_LEFT_23_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_23_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_23=636,20
PRIMER_RIGHT_23=1411,22
PRIMER_LEFT_23_TM=60.096
PRIMER_RIGHT_23_TM=60.012
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.2000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=776
PRIMER_PAIR_24_PENALTY=2.145894
PRIMER_LEFT_24_PENALTY=0.133741
PRIMER_RIGHT_24_PENALTY=2.012153
PRIMER_LEFT_24_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_24_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_24=446,20
PRIMER_RIGHT_24=1410,22
PRIMER_LEFT_24_TM=60.134
PRIMER_RIGHT_24_TM=60.012
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=5.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=965
PRIMER_PAIR_25_PENALTY=2.145894
PRIMER_LEFT_25_PENALTY=0.133741
PRIMER_RIGHT_25_PENALTY=2.012153
PRIMER_LEFT_25_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_25_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_25=446,20
PRIMER_RIGHT_25=1411,22
PRIMER_LEFT_25_TM=60.134
PRIMER_RIGHT_25_TM=60.012
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=5.4000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=966
PRIMER_PAIR_26_PENALTY=2.263053
PRIMER_LEFT_26_PENALTY=0.668065
PRIMER_RIGHT_26_PENALTY=1.594988
PRIMER_LEFT_26_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_26_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_26=528,20
PRIMER_RIGHT_26=1438,21
PRIMER_LEFT_26_TM=60.668
PRIMER_RIGHT_26_TM=60.595
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=911
PRIMER_PAIR_27_PENALTY=2.263053
PRIMER_LEFT_27_PENALTY=0.668065
PRIMER_RIGHT_27_PENALTY=1.594988
PRIMER_LEFT_27_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_27_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_27=528,20
PRIMER_RIGHT_27=1439,21
PRIMER_LEFT_27_TM=60.668
PRIMER_RIGHT_27_TM=60.595
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=912
PRIMER_PAIR_28_PENALTY=2.296575
PRIMER_LEFT_28_PENALTY=0.031122
PRIMER_RIGHT_28_PENALTY=2.265453
PRIMER_LEFT_28_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_28_SEQUENCE=GCCAATGAAAGAATCCTGAA
PRIMER_LEFT_28=624,20
PRIMER_RIGHT_28=1484,20
PRIMER_LEFT_28_TM=60.031
PRIMER_RIGHT_28_TM=57.735
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=861
PRIMER_PAIR_29_PENALTY=2.361082
PRIMER_LEFT_29_PENALTY=0.095629
PRIMER_RIGHT_29_PENALTY=2.265453
PRIMER_LEFT_29_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_29_SEQUENCE=GCCAATGAAAGAATCCTGAA
PRIMER_LEFT_29=636,20
PRIMER_RIGHT_29=1484,20
PRIMER_LEFT_29_TM=60.096
PRIMER_RIGHT_29_TM=57.735
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=10.2000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=849
PRIMER_PAIR_30_PENALTY=2.375857
PRIMER_LEFT_30_PENALTY=1.946991
PRIMER_RIGHT_30_PENALTY=0.428866
PRIMER_LEFT_30_SEQUENCE=ACTCCAACACCATCCGCTACT
PRIMER_RIGHT_30_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_30=445,21
PRIMER_RIGHT_30=1438,20
PRIMER_LEFT_30_TM=60.947
PRIMER_RIGHT_30_TM=59.571
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=5.4000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=994
PRIMER_PAIR_31_PENALTY=2.394367
PRIMER_LEFT_31_PENALTY=0.003322
PRIMER_RIGHT_31_PENALTY=2.391045
PRIMER_LEFT_31_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_31_SEQUENCE=GTCTCCACGTGACAAAACCT
PRIMER_LEFT_31=445,20
PRIMER_RIGHT_31=1437,20
PRIMER_LEFT_31_TM=59.997
PRIMER_RIGHT_31_TM=57.609
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=993
PRIMER_PAIR_32_PENALTY=2.453124
PRIMER_LEFT_32_PENALTY=2.024258
PRIMER_RIGHT_32_PENALTY=0.428866
PRIMER_LEFT_32_SEQUENCE=GCTGGTTCACCACTTCCCTC
PRIMER_RIGHT_32_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_32=493,20
PRIMER_RIGHT_32=1438,20
PRIMER_LEFT_32_TM=62.024
PRIMER_RIGHT_32_TM=59.571
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=946
PRIMER_PAIR_33_PENALTY=2.467318
PRIMER_LEFT_33_PENALTY=0.668065
PRIMER_RIGHT_33_PENALTY=1.799253
PRIMER_LEFT_33_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_33_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_33=528,20
PRIMER_RIGHT_33=1410,21
PRIMER_LEFT_33_TM=60.668
PRIMER_RIGHT_33_TM=59.201
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=883
PRIMER_PAIR_34_PENALTY=2.486673
PRIMER_LEFT_34_PENALTY=0.095629
PRIMER_RIGHT_34_PENALTY=2.391045
PRIMER_LEFT_34_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_34_SEQUENCE=GTCTCCACGTGACAAAACCT
PRIMER_LEFT_34=636,20
PRIMER_RIGHT_34=1437,20
PRIMER_LEFT_34_TM=60.096
PRIMER_RIGHT_34_TM=57.609
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.2000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=802
PRIMER_PAIR_35_PENALTY=2.524785
PRIMER_LEFT_35_PENALTY=0.133741
PRIMER_RIGHT_35_PENALTY=2.391045
PRIMER_LEFT_35_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_35_SEQUENCE=GTCTCCACGTGACAAAACCT
PRIMER_LEFT_35=446,20
PRIMER_RIGHT_35=1437,20
PRIMER_LEFT_35_TM=60.134
PRIMER_RIGHT_35_TM=57.609
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=5.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=2.593204
PRIMER_LEFT_36_PENALTY=0.031122
PRIMER_RIGHT_36_PENALTY=2.562082
PRIMER_LEFT_36_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_36_SEQUENCE=GAGCCAATGAAAGAATCCTGAA
PRIMER_LEFT_36=624,20
PRIMER_RIGHT_36=1486,22
PRIMER_LEFT_36_TM=60.031
PRIMER_RIGHT_36_TM=60.562
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.2000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=863
PRIMER_PAIR_37_PENALTY=2.657711
PRIMER_LEFT_37_PENALTY=0.095629
PRIMER_RIGHT_37_PENALTY=2.562082
PRIMER_LEFT_37_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_37_SEQUENCE=GAGCCAATGAAAGAATCCTGAA
PRIMER_LEFT_37=636,20
PRIMER_RIGHT_37=1486,22
PRIMER_LEFT_37_TM=60.096
PRIMER_RIGHT_37_TM=60.562
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.2000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=851
PRIMER_PAIR_38_PENALTY=2.661557
PRIMER_LEFT_38_PENALTY=1.066570
PRIMER_RIGHT_38_PENALTY=1.594988
PRIMER_LEFT_38_SEQUENCE=TCCAACACCATCCGCTACTC
PRIMER_RIGHT_38_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_38=447,20
PRIMER_RIGHT_38=1438,21
PRIMER_LEFT_38_TM=61.067
PRIMER_RIGHT_38_TM=60.595
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.4000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=992
PRIMER_PAIR_39_PENALTY=2.661557
PRIMER_LEFT_39_PENALTY=1.066570
PRIMER_RIGHT_39_PENALTY=1.594988
PRIMER_LEFT_39_SEQUENCE=TCCAACACCATCCGCTACTC
PRIMER_RIGHT_39_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_39=447,20
PRIMER_RIGHT_39=1439,21
PRIMER_LEFT_39_TM=61.067
PRIMER_RIGHT_39_TM=60.595
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=5.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=993
PRIMER_PAIR_40_PENALTY=2.669071
PRIMER_LEFT_40_PENALTY=2.240205
PRIMER_RIGHT_40_PENALTY=0.428866
PRIMER_LEFT_40_SEQUENCE=CCGGATTTGTATTCGTCGCT
PRIMER_RIGHT_40_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_40=635,20
PRIMER_RIGHT_40=1438,20
PRIMER_LEFT_40_TM=62.240
PRIMER_RIGHT_40_TM=59.571
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=804
PRIMER_PAIR_41_PENALTY=2.680218
PRIMER_LEFT_41_PENALTY=0.668065
PRIMER_RIGHT_41_PENALTY=2.012153
PRIMER_LEFT_41_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_41_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_41=528,20
PRIMER_RIGHT_41=1410,22
PRIMER_LEFT_41_TM=60.668
PRIMER_RIGHT_41_TM=60.012
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=883
PRIMER_PAIR_42_PENALTY=2.680218
PRIMER_LEFT_42_PENALTY=0.668065
PRIMER_RIGHT_42_PENALTY=2.012153
PRIMER_LEFT_42_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_42_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_42=528,20
PRIMER_RIGHT_42=1411,22
PRIMER_LEFT_42_TM=60.668
PRIMER_RIGHT_42_TM=60.012
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=884
PRIMER_PAIR_43_PENALTY=2.698268
PRIMER_LEFT_43_PENALTY=0.003322
PRIMER_RIGHT_43_PENALTY=2.694946
PRIMER_LEFT_43_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_43_SEQUENCE=CCTTTCTCACCAAACCAAAG
PRIMER_LEFT_43=445,20
PRIMER_RIGHT_43=1410,20
PRIMER_LEFT_43_TM=59.997
PRIMER_RIGHT_43_TM=57.305
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=7.3000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=2.725077
PRIMER_LEFT_44_PENALTY=2.296211
PRIMER_RIGHT_44_PENALTY=0.428866
PRIMER_LEFT_44_SEQUENCE=ACACCATCCGCTACTCCCAC
PRIMER_RIGHT_44_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_44=451,20
PRIMER_RIGHT_44=1438,20
PRIMER_LEFT_44_TM=62.296
PRIMER_RIGHT_44_TM=59.571
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=988
PRIMER_PAIR_45_PENALTY=2.726068
PRIMER_LEFT_45_PENALTY=0.031122
PRIMER_RIGHT_45_PENALTY=2.694946
PRIMER_LEFT_45_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_45_SEQUENCE=CCTTTCTCACCAAACCAAAG
PRIMER_LEFT_45=624,20
PRIMER_RIGHT_45=1410,20
PRIMER_LEFT_45_TM=60.031
PRIMER_RIGHT_45_TM=57.305
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.2000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=787
PRIMER_PAIR_46_PENALTY=2.738136
PRIMER_LEFT_46_PENALTY=2.309270
PRIMER_RIGHT_46_PENALTY=0.428866
PRIMER_LEFT_46_SEQUENCE=AACTCCAACACCATCCGCTAC
PRIMER_RIGHT_46_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_46=444,21
PRIMER_RIGHT_46=1438,20
PRIMER_LEFT_46_TM=61.309
PRIMER_RIGHT_46_TM=59.571
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=995
PRIMER_PAIR_47_PENALTY=2.789804
PRIMER_LEFT_47_PENALTY=0.003322
PRIMER_RIGHT_47_PENALTY=2.786482
PRIMER_LEFT_47_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_47_SEQUENCE=ACCTTTCTCACCAAACCAAAG
PRIMER_LEFT_47=445,20
PRIMER_RIGHT_47=1411,21
PRIMER_LEFT_47_TM=59.997
PRIMER_RIGHT_47_TM=58.214
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=2.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=967
PRIMER_PAIR_48_PENALTY=2.790575
PRIMER_LEFT_48_PENALTY=0.095629
PRIMER_RIGHT_48_PENALTY=2.694946
PRIMER_LEFT_48_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_48_SEQUENCE=CCTTTCTCACCAAACCAAAG
PRIMER_LEFT_48=636,20
PRIMER_RIGHT_48=1410,20
PRIMER_LEFT_48_TM=60.096
PRIMER_RIGHT_48_TM=57.305
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=10.2000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=775
PRIMER_PAIR_49_PENALTY=2.793692
PRIMER_LEFT_49_PENALTY=2.364827
PRIMER_RIGHT_49_PENALTY=0.428866
PRIMER_LEFT_49_SEQUENCE=GGATTTGTATTCGTCGCTTCTT
PRIMER_RIGHT_49_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_49=637,22
PRIMER_RIGHT_49=1438,20
PRIMER_LEFT_49_TM=59.635
PRIMER_RIGHT_49_TM=59.571
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=802
=
SEQUENCE_ID=MAGIC.175_1_14251801_14252199_wil-2_ct-1_760452_13907_TRUE
SEQUENCE_TARGET=950,498
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_14005267_14005282_ct-1_bur-0_21982_8746_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_7765474_7766115_edi-0_po-0_117333_385064_TRUE
SEQUENCE_TARGET=950,741
SEQUENCE_TEMPLATE=CATTTACCCAAANGTAGNATGTTCAATAAATGGTNATGCTGANTGATGCTATTACAAAAATTGTTTGNCNACAANAANNNNNNNNAAAAANAAAAANAAAGATGGAANTTCCTNTTTGCTAATTTGCNTCTATNAGATTTGGATTTNNACGCAACATTCTACATAAGATATGATTTGCCACAACNAATTTTGATCCGATTTTTTGAAANTTTTCTAAAAATATNACTATGATTTAACATTTCCTNTTTNTNTTNTATATTAAANTTTTTATCATTANTTTANTCTTTTTTNNNNTTTTNGTTAAATTTATCATTGTTTTACTTGAAAGTTGGAAACTATNGCGTCGAATGGAGACAGAAAACTTAACGGCGGGAAAGCAAAAAATAATCATANGAATTTGGCGACAAAACAAGATTGTAACTTGTAAGAANAAAANTAACTTTTTATCAAAAAGACAAAATNANNNNNTAAAANANGAAGAAAAATGGAAAGATGATCTTCATTATTGANTATTCTCACAAATTAGGAAACATAAATAAATAAANNANNANNNNANNANANTTCANAATNTATACACTACGCATGCCATACACAACACATATATAAAGAAAGAAAGTGTTTTAAAAGAGAGCAAACTCAGAAGAAACAACAAAACCTGAAATCATTNANNTTCTTCTTCTTCACAATTTTNTCTCTGATCGTTTTTGGGTTTCTCATTTNTAAGTAAAACCTCTAATAAGAAACNTTTTTCACATCACCAGATCCGATCTTGAGCTTTNGCACCAGCNNNAAAAAANNAGGTTNTCTTCTATCTTTNCTTCTACCTTTCTTGATCTATCAGATTTGGTAACATGCATGTGAGNNNNNNTACTTGTTAANAATAATCATACATAAGAATCTTTCGATTCACAAATGTTTCTTGGGAAAANAAAAGTGGGGATTNGAGTTTTGTATTTTCATNCGTTTTCTGGAATCCANACACTAAATTTCTTNNTGCAAANCCCNAAATGTTAAATTAGTTTTTCAAAAGATTTGAGGCAGAGAGGGACCAGTCAATANTCACTTTTCCTTTNTTTTTTTNTCACTGTCTTTGCTTTCTGCTAATTATCTAACTTTTCCAACAGATTAGACGTAACTTTAACANAATTTAAACCTNNNNNNNNNNNNNATTAAGNTAGATATATGTAGTTTTTTAGTAATAACNTATGCNAGACTTCCANGTACATGATTCTTCTCTATGCTTCATTTATAAGTTTTCTTTCCTTTTCNGTTTTTACTTACATAACNAAATTCAANTANNNNNNNNTTTTTNNNTAANANAAAAGCTTATTTTATTGATNGGAACAACAACCNNNNNNNCCAGGGCATNCTTGNNNNNNNNTGATGATGAGCTTAGGATTAGAATAATTACCACTACNANNTTTNTATCTTATTTCTCTTANAATTNAATATTTNTTCTAATTAAAATANTTTTAGNGAATTTNAGTTTNTTACAAAAAAAGCTGTTGTNNNNNNNNNNNNAACNNNNNNNNNNNTTTGGNATTTATNTAANTTCTGCTTCATCACCATCANTATGTTAAATTANTTNTTTTTTTCTTTGTCGCCGGTTTTTAAACTCTTGTTAATTTNNAAGAATTTANCNTTTTAATAANTAACTAGTACATCGANTGAGNTATTTACANATGTCAAATTNGNTNNCATTCTTTAACATANAANATAGNTTTTTCATATCAAATTTCNGCTGATCCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTAACCACTAACCACGGGTCTCATAAAGTGGACTTGTGAAGCTCCAAAAATCAAGTTCGCTTTTCTCAATTTCTGGTNNNNNNNNNNNATAAAAAGNAAAAAGTGTATGAATAGCATTAACAATTTGCTCACTTGTATAAAGATTCGTTAGTCCAATAGCGTCCGTACACGTTTCATTTAGTTTGCAATTTGTATACATTCATCCCCCAAAACAATCTCTAAGGATTTGTTTCNTTTTTTATCTCTTCTTTAAGCTTTCGTTTCTCTNTCAATTATAAAGTTTCTTCGCCACTTCTTAGTTTTATTATTGATATCTCTNTTCAAAAAGATTCTTCCTTTTCTATNNGAGTTCAATAATTTTAAGTTTGATATTTNTGTCTTATGNTTATACAATTTCATTCANATTATATAATGGAGATTTCGTTGACGTTTATGTGAGTAACATCAATAAAAGCATGCCTTNNAAAGGTTTTTTGTTAGGTAAACTTGCACTCCACGTATTCTTTGTTATGTTCTACTTAANAAAAAAAAAGATACATAACAAATTCTTTCTGTTGAGAAACGAATTAAGTGTGAATTAATTAAGAGAATAAAACACGTNTGTTTCTCATGTTATACATGATTTCTCTCATCTCATCTAATGCAAGNGCTTTAATTTTCATNGNTTCTTGTTTTAGGTTCTTTGTTCTACTGAAGGAAGACAACTAAACGCTACTNGAGTGTCTTTCTTNTGTTTAGTCCGGTCATCTATTNATTACCAACAATCTCCCAAGNACCGAGAGGTTCNAGAGACTGACAAATCTCCTNGTTCCATTCGTTCGAGAGAACAAAAATCTCTTTTTCTTTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.742764
PRIMER_LEFT_0_PENALTY=3.586704
PRIMER_RIGHT_0_PENALTY=0.156061
PRIMER_LEFT_0_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_0_SEQUENCE=ATGAGACCCGTGGTTAGTGG
PRIMER_LEFT_0=902,22
PRIMER_RIGHT_0=1817,20
PRIMER_LEFT_0_TM=61.587
PRIMER_RIGHT_0_TM=59.844
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=916
PRIMER_PAIR_1_PENALTY=4.008486
PRIMER_LEFT_1_PENALTY=3.586704
PRIMER_RIGHT_1_PENALTY=0.421782
PRIMER_LEFT_1_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_1_SEQUENCE=TGAGACCCGTGGTTAGTGGT
PRIMER_LEFT_1=902,22
PRIMER_RIGHT_1=1816,20
PRIMER_LEFT_1_TM=61.587
PRIMER_RIGHT_1_TM=60.422
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=915
PRIMER_PAIR_2_PENALTY=4.669458
PRIMER_LEFT_2_PENALTY=3.586704
PRIMER_RIGHT_2_PENALTY=1.082754
PRIMER_LEFT_2_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_2_SEQUENCE=GAGACCCGTGGTTAGTGGTT
PRIMER_LEFT_2=902,22
PRIMER_RIGHT_2=1815,20
PRIMER_LEFT_2_TM=61.587
PRIMER_RIGHT_2_TM=58.917
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=914
PRIMER_PAIR_3_PENALTY=4.725896
PRIMER_LEFT_3_PENALTY=3.586704
PRIMER_RIGHT_3_PENALTY=1.139192
PRIMER_LEFT_3_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_3_SEQUENCE=TATGAGACCCGTGGTTAGTGG
PRIMER_LEFT_3=902,22
PRIMER_RIGHT_3=1818,21
PRIMER_LEFT_3_TM=61.587
PRIMER_RIGHT_3_TM=59.861
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=917
PRIMER_PAIR_4_PENALTY=5.261952
PRIMER_LEFT_4_PENALTY=3.586704
PRIMER_RIGHT_4_PENALTY=1.675248
PRIMER_LEFT_4_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_4_SEQUENCE=ATGAGACCCGTGGTTAGTGGT
PRIMER_LEFT_4=902,22
PRIMER_RIGHT_4=1817,21
PRIMER_LEFT_4_TM=61.587
PRIMER_RIGHT_4_TM=60.675
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=916
PRIMER_PAIR_5_PENALTY=5.493454
PRIMER_LEFT_5_PENALTY=3.586704
PRIMER_RIGHT_5_PENALTY=1.906750
PRIMER_LEFT_5_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_5_SEQUENCE=CCAGAAATTGAGAAAAGCGA
PRIMER_LEFT_5=902,22
PRIMER_RIGHT_5=1869,20
PRIMER_LEFT_5_TM=61.587
PRIMER_RIGHT_5_TM=58.093
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=10.0000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=968
PRIMER_PAIR_6_PENALTY=5.823401
PRIMER_LEFT_6_PENALTY=3.586704
PRIMER_RIGHT_6_PENALTY=2.236697
PRIMER_LEFT_6_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_6_SEQUENCE=CCAGAAATTGAGAAAAGCGAAC
PRIMER_LEFT_6=902,22
PRIMER_RIGHT_6=1869,22
PRIMER_LEFT_6_TM=61.587
PRIMER_RIGHT_6_TM=60.237
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.0000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=968
PRIMER_PAIR_7_PENALTY=6.247417
PRIMER_LEFT_7_PENALTY=3.586704
PRIMER_RIGHT_7_PENALTY=2.660713
PRIMER_LEFT_7_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_7_SEQUENCE=TATGAGACCCGTGGTTAGTGGT
PRIMER_LEFT_7=902,22
PRIMER_RIGHT_7=1818,22
PRIMER_LEFT_7_TM=61.587
PRIMER_RIGHT_7_TM=60.661
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=10.0000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=917
PRIMER_PAIR_8_PENALTY=6.264072
PRIMER_LEFT_8_PENALTY=3.586704
PRIMER_RIGHT_8_PENALTY=2.677368
PRIMER_LEFT_8_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_8_SEQUENCE=GACCCGTGGTTAGTGGTTACTT
PRIMER_LEFT_8=902,22
PRIMER_RIGHT_8=1813,22
PRIMER_LEFT_8_TM=61.587
PRIMER_RIGHT_8_TM=59.323
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.0000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=912
PRIMER_PAIR_9_PENALTY=6.317877
PRIMER_LEFT_9_PENALTY=3.586704
PRIMER_RIGHT_9_PENALTY=2.731173
PRIMER_LEFT_9_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_9_SEQUENCE=TGAGACCCGTGGTTAGTGGTT
PRIMER_LEFT_9=902,22
PRIMER_RIGHT_9=1816,21
PRIMER_LEFT_9_TM=61.587
PRIMER_RIGHT_9_TM=61.731
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=10.0000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=915
PRIMER_PAIR_10_PENALTY=6.490817
PRIMER_LEFT_10_PENALTY=3.586704
PRIMER_RIGHT_10_PENALTY=2.904113
PRIMER_LEFT_10_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_10_SEQUENCE=AGCGAACTTGATTTTTGGAG
PRIMER_LEFT_10=902,22
PRIMER_RIGHT_10=1854,20
PRIMER_LEFT_10_TM=61.587
PRIMER_RIGHT_10_TM=57.096
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=10.0000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=953
PRIMER_PAIR_11_PENALTY=6.569308
PRIMER_LEFT_11_PENALTY=3.586704
PRIMER_RIGHT_11_PENALTY=2.982604
PRIMER_LEFT_11_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_11_SEQUENCE=TTTATGAGACCCGTGGTTAGTG
PRIMER_LEFT_11=902,22
PRIMER_RIGHT_11=1820,22
PRIMER_LEFT_11_TM=61.587
PRIMER_RIGHT_11_TM=59.017
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.0000
PRIMER_RIGHT_11_END_STABILITY=5.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=919
PRIMER_PAIR_12_PENALTY=6.652080
PRIMER_LEFT_12_PENALTY=3.586704
PRIMER_RIGHT_12_PENALTY=3.065377
PRIMER_LEFT_12_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_12_SEQUENCE=CTTTATGAGACCCGTGGTTAGTG
PRIMER_LEFT_12=902,22
PRIMER_RIGHT_12=1821,23
PRIMER_LEFT_12_TM=61.587
PRIMER_RIGHT_12_TM=59.935
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.0000
PRIMER_RIGHT_12_END_STABILITY=5.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=920
PRIMER_PAIR_13_PENALTY=6.652080
PRIMER_LEFT_13_PENALTY=3.586704
PRIMER_RIGHT_13_PENALTY=3.065377
PRIMER_LEFT_13_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_13_SEQUENCE=CACTTTATGAGACCCGTGGTTAG
PRIMER_LEFT_13=902,22
PRIMER_RIGHT_13=1823,23
PRIMER_LEFT_13_TM=61.587
PRIMER_RIGHT_13_TM=59.935
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=5.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=922
PRIMER_PAIR_14_PENALTY=6.672208
PRIMER_LEFT_14_PENALTY=3.586704
PRIMER_RIGHT_14_PENALTY=3.085505
PRIMER_LEFT_14_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_14_SEQUENCE=CACAAGTCCACTTTATGAGACCC
PRIMER_LEFT_14=902,22
PRIMER_RIGHT_14=1831,23
PRIMER_LEFT_14_TM=61.587
PRIMER_RIGHT_14_TM=59.914
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=10.0000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=930
PRIMER_PAIR_15_PENALTY=6.719551
PRIMER_LEFT_15_PENALTY=3.586704
PRIMER_RIGHT_15_PENALTY=3.132848
PRIMER_LEFT_15_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_15_SEQUENCE=TTATGAGACCCGTGGTTAGTGG
PRIMER_LEFT_15=902,22
PRIMER_RIGHT_15=1819,22
PRIMER_LEFT_15_TM=61.587
PRIMER_RIGHT_15_TM=61.133
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=918
PRIMER_PAIR_16_PENALTY=6.793369
PRIMER_LEFT_16_PENALTY=3.586704
PRIMER_RIGHT_16_PENALTY=3.206665
PRIMER_LEFT_16_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_16_SEQUENCE=AGACCCGTGGTTAGTGGTTACTT
PRIMER_LEFT_16=902,22
PRIMER_RIGHT_16=1814,23
PRIMER_LEFT_16_TM=61.587
PRIMER_RIGHT_16_TM=60.207
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=10.0000
PRIMER_RIGHT_16_END_STABILITY=5.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=913
PRIMER_PAIR_17_PENALTY=6.965164
PRIMER_LEFT_17_PENALTY=3.586704
PRIMER_RIGHT_17_PENALTY=3.378460
PRIMER_LEFT_17_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_17_SEQUENCE=TTATGAGACCCGTGGTTAGTG
PRIMER_LEFT_17=902,22
PRIMER_RIGHT_17=1819,21
PRIMER_LEFT_17_TM=61.587
PRIMER_RIGHT_17_TM=57.622
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=10.0000
PRIMER_RIGHT_17_END_STABILITY=5.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=918
PRIMER_PAIR_18_PENALTY=6.973903
PRIMER_LEFT_18_PENALTY=3.586704
PRIMER_RIGHT_18_PENALTY=3.387200
PRIMER_LEFT_18_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_18_SEQUENCE=CCCGTGGTTAGTGGTTACTT
PRIMER_LEFT_18=902,22
PRIMER_RIGHT_18=1811,20
PRIMER_LEFT_18_TM=61.587
PRIMER_RIGHT_18_TM=56.613
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=10.0000
PRIMER_RIGHT_18_END_STABILITY=5.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=910
PRIMER_PAIR_19_PENALTY=7.053973
PRIMER_LEFT_19_PENALTY=3.586704
PRIMER_RIGHT_19_PENALTY=3.467270
PRIMER_LEFT_19_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_19_SEQUENCE=ACCCGTGGTTAGTGGTTACTT
PRIMER_LEFT_19=902,22
PRIMER_RIGHT_19=1812,21
PRIMER_LEFT_19_TM=61.587
PRIMER_RIGHT_19_TM=57.533
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=10.0000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=911
PRIMER_PAIR_20_PENALTY=7.490746
PRIMER_LEFT_20_PENALTY=3.586704
PRIMER_RIGHT_20_PENALTY=3.904042
PRIMER_LEFT_20_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_20_SEQUENCE=ATGAGACCCGTGGTTAGTGGTT
PRIMER_LEFT_20=902,22
PRIMER_RIGHT_20=1817,22
PRIMER_LEFT_20_TM=61.587
PRIMER_RIGHT_20_TM=61.904
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=10.0000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=916
PRIMER_PAIR_21_PENALTY=7.523345
PRIMER_LEFT_21_PENALTY=3.586704
PRIMER_RIGHT_21_PENALTY=3.936642
PRIMER_LEFT_21_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_21_SEQUENCE=TATGAGACCCGTGGTTAGTG
PRIMER_LEFT_21=902,22
PRIMER_RIGHT_21=1818,20
PRIMER_LEFT_21_TM=61.587
PRIMER_RIGHT_21_TM=56.063
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=10.0000
PRIMER_RIGHT_21_END_STABILITY=5.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=917
PRIMER_PAIR_22_PENALTY=8.171704
PRIMER_LEFT_22_PENALTY=3.586704
PRIMER_RIGHT_22_PENALTY=4.585000
PRIMER_LEFT_22_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_22_SEQUENCE=CTTTATGAGACCCGTGGTTAG
PRIMER_LEFT_22=902,22
PRIMER_RIGHT_22=1821,21
PRIMER_LEFT_22_TM=61.587
PRIMER_RIGHT_22_TM=56.415
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=10.0000
PRIMER_RIGHT_22_END_STABILITY=5.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=920
PRIMER_PAIR_23_PENALTY=8.251006
PRIMER_LEFT_23_PENALTY=3.586704
PRIMER_RIGHT_23_PENALTY=4.664302
PRIMER_LEFT_23_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_23_SEQUENCE=ACTTTATGAGACCCGTGGTTAGTG
PRIMER_LEFT_23=902,22
PRIMER_RIGHT_23=1822,24
PRIMER_LEFT_23_TM=61.587
PRIMER_RIGHT_23_TM=60.664
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.833
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.0000
PRIMER_RIGHT_23_END_STABILITY=5.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=921
PRIMER_PAIR_24_PENALTY=8.251006
PRIMER_LEFT_24_PENALTY=3.586704
PRIMER_RIGHT_24_PENALTY=4.664302
PRIMER_LEFT_24_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_24_SEQUENCE=CACTTTATGAGACCCGTGGTTAGT
PRIMER_LEFT_24=902,22
PRIMER_RIGHT_24=1823,24
PRIMER_LEFT_24_TM=61.587
PRIMER_RIGHT_24_TM=60.664
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=45.833
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=10.0000
PRIMER_RIGHT_24_END_STABILITY=5.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=922
PRIMER_PAIR_25_PENALTY=8.265083
PRIMER_LEFT_25_PENALTY=3.586704
PRIMER_RIGHT_25_PENALTY=4.678379
PRIMER_LEFT_25_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_25_SEQUENCE=CTTTATGAGACCCGTGGTTAGT
PRIMER_LEFT_25=902,22
PRIMER_RIGHT_25=1821,22
PRIMER_LEFT_25_TM=61.587
PRIMER_RIGHT_25_TM=57.322
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=10.0000
PRIMER_RIGHT_25_END_STABILITY=5.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=920
PRIMER_PAIR_26_PENALTY=8.265083
PRIMER_LEFT_26_PENALTY=3.586704
PRIMER_RIGHT_26_PENALTY=4.678379
PRIMER_LEFT_26_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_26_SEQUENCE=ACTTTATGAGACCCGTGGTTAG
PRIMER_LEFT_26=902,22
PRIMER_RIGHT_26=1822,22
PRIMER_LEFT_26_TM=61.587
PRIMER_RIGHT_26_TM=57.322
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.0000
PRIMER_RIGHT_26_END_STABILITY=5.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=921
PRIMER_PAIR_27_PENALTY=8.323297
PRIMER_LEFT_27_PENALTY=3.586704
PRIMER_RIGHT_27_PENALTY=4.736594
PRIMER_LEFT_27_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_27_SEQUENCE=TTTATGAGACCCGTGGTTAG
PRIMER_LEFT_27=902,22
PRIMER_RIGHT_27=1820,20
PRIMER_LEFT_27_TM=61.587
PRIMER_RIGHT_27_TM=55.263
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=10.0000
PRIMER_RIGHT_27_END_STABILITY=5.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=919
PRIMER_PAIR_28_PENALTY=8.330086
PRIMER_LEFT_28_PENALTY=3.586704
PRIMER_RIGHT_28_PENALTY=4.743382
PRIMER_LEFT_28_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_28_SEQUENCE=TTTATGAGACCCGTGGTTAGT
PRIMER_LEFT_28=902,22
PRIMER_RIGHT_28=1820,21
PRIMER_LEFT_28_TM=61.587
PRIMER_RIGHT_28_TM=56.257
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=10.0000
PRIMER_RIGHT_28_END_STABILITY=5.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=919
PRIMER_PAIR_29_PENALTY=8.341966
PRIMER_LEFT_29_PENALTY=3.586704
PRIMER_RIGHT_29_PENALTY=4.755263
PRIMER_LEFT_29_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_29_SEQUENCE=CAAGTCCACTTTATGAGACCC
PRIMER_LEFT_29=902,22
PRIMER_RIGHT_29=1829,21
PRIMER_LEFT_29_TM=61.587
PRIMER_RIGHT_29_TM=56.245
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=10.0000
PRIMER_RIGHT_29_END_STABILITY=9.1000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=928
PRIMER_PAIR_30_PENALTY=8.391965
PRIMER_LEFT_30_PENALTY=3.586704
PRIMER_RIGHT_30_PENALTY=4.805261
PRIMER_LEFT_30_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_30_SEQUENCE=ACAAGTCCACTTTATGAGACCC
PRIMER_LEFT_30=902,22
PRIMER_RIGHT_30=1830,22
PRIMER_LEFT_30_TM=61.587
PRIMER_RIGHT_30_TM=57.195
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=10.0000
PRIMER_RIGHT_30_END_STABILITY=9.1000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=929
PRIMER_PAIR_31_PENALTY=8.423013
PRIMER_LEFT_31_PENALTY=3.586704
PRIMER_RIGHT_31_PENALTY=4.836309
PRIMER_LEFT_31_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_31_SEQUENCE=ACTTTATGAGACCCGTGGTTAGT
PRIMER_LEFT_31=902,22
PRIMER_RIGHT_31=1822,23
PRIMER_LEFT_31_TM=61.587
PRIMER_RIGHT_31_TM=58.164
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=43.478
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=10.0000
PRIMER_RIGHT_31_END_STABILITY=5.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=921
PRIMER_PAIR_32_PENALTY=8.433732
PRIMER_LEFT_32_PENALTY=3.586704
PRIMER_RIGHT_32_PENALTY=4.847028
PRIMER_LEFT_32_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_32_SEQUENCE=TATGAGACCCGTGGTTAGTGGTT
PRIMER_LEFT_32=902,22
PRIMER_RIGHT_32=1818,23
PRIMER_LEFT_32_TM=61.587
PRIMER_RIGHT_32_TM=61.847
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=47.826
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=10.0000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=917
PRIMER_PAIR_33_PENALTY=8.433732
PRIMER_LEFT_33_PENALTY=3.586704
PRIMER_RIGHT_33_PENALTY=4.847028
PRIMER_LEFT_33_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_33_SEQUENCE=TTATGAGACCCGTGGTTAGTGGT
PRIMER_LEFT_33=902,22
PRIMER_RIGHT_33=1819,23
PRIMER_LEFT_33_TM=61.587
PRIMER_RIGHT_33_TM=61.847
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=47.826
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=10.0000
PRIMER_RIGHT_33_END_STABILITY=7.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=918
PRIMER_PAIR_34_PENALTY=8.866307
PRIMER_LEFT_34_PENALTY=3.586704
PRIMER_RIGHT_34_PENALTY=5.279604
PRIMER_LEFT_34_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_34_SEQUENCE=TTTATGAGACCCGTGGTTAGTGG
PRIMER_LEFT_34=902,22
PRIMER_RIGHT_34=1820,23
PRIMER_LEFT_34_TM=61.587
PRIMER_RIGHT_34_TM=62.280
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=47.826
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.0000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=919
PRIMER_PAIR_35_PENALTY=9.204511
PRIMER_LEFT_35_PENALTY=3.586704
PRIMER_RIGHT_35_PENALTY=5.617807
PRIMER_LEFT_35_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_35_SEQUENCE=TCACAAGTCCACTTTATGAGACCC
PRIMER_LEFT_35=902,22
PRIMER_RIGHT_35=1832,24
PRIMER_LEFT_35_TM=61.587
PRIMER_RIGHT_35_TM=61.618
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=45.833
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=10.0000
PRIMER_RIGHT_35_END_STABILITY=9.1000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=931
PRIMER_PAIR_36_PENALTY=9.390583
PRIMER_LEFT_36_PENALTY=3.586704
PRIMER_RIGHT_36_PENALTY=5.803880
PRIMER_LEFT_36_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_36_SEQUENCE=GAGACCCGTGGTTAGTGGTTACTT
PRIMER_LEFT_36=902,22
PRIMER_RIGHT_36=1815,24
PRIMER_LEFT_36_TM=61.587
PRIMER_RIGHT_36_TM=61.804
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.0000
PRIMER_RIGHT_36_END_STABILITY=5.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=914
PRIMER_PAIR_37_PENALTY=10.507212
PRIMER_LEFT_37_PENALTY=3.586704
PRIMER_RIGHT_37_PENALTY=6.920508
PRIMER_LEFT_37_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_37_SEQUENCE=TTATGAGACCCGTGGTTAGTGGTT
PRIMER_LEFT_37=902,22
PRIMER_RIGHT_37=1819,24
PRIMER_LEFT_37_TM=61.587
PRIMER_RIGHT_37_TM=62.921
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=45.833
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=10.0000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=918
PRIMER_PAIR_38_PENALTY=10.507212
PRIMER_LEFT_38_PENALTY=3.586704
PRIMER_RIGHT_38_PENALTY=6.920508
PRIMER_LEFT_38_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_38_SEQUENCE=TTTATGAGACCCGTGGTTAGTGGT
PRIMER_LEFT_38=902,22
PRIMER_RIGHT_38=1820,24
PRIMER_LEFT_38_TM=61.587
PRIMER_RIGHT_38_TM=62.921
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=45.833
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=10.0000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=919
PRIMER_PAIR_39_PENALTY=10.601992
PRIMER_LEFT_39_PENALTY=3.586704
PRIMER_RIGHT_39_PENALTY=7.015289
PRIMER_LEFT_39_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_39_SEQUENCE=CTTTATGAGACCCGTGGTTAGTGG
PRIMER_LEFT_39=902,22
PRIMER_RIGHT_39=1821,24
PRIMER_LEFT_39_TM=61.587
PRIMER_RIGHT_39_TM=63.015
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=10.0000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=920
PRIMER_PAIR_40_PENALTY=10.601992
PRIMER_LEFT_40_PENALTY=3.586704
PRIMER_RIGHT_40_PENALTY=7.015289
PRIMER_LEFT_40_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_40_SEQUENCE=CCACTTTATGAGACCCGTGGTTAG
PRIMER_LEFT_40=902,22
PRIMER_RIGHT_40=1824,24
PRIMER_LEFT_40_TM=61.587
PRIMER_RIGHT_40_TM=63.015
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=10.0000
PRIMER_RIGHT_40_END_STABILITY=5.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=923
PRIMER_PAIR_41_PENALTY=11.297338
PRIMER_LEFT_41_PENALTY=3.586704
PRIMER_RIGHT_41_PENALTY=7.710634
PRIMER_LEFT_41_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_41_SEQUENCE=TTCACAAGTCCACTTTATGAGACCC
PRIMER_LEFT_41=902,22
PRIMER_RIGHT_41=1833,25
PRIMER_LEFT_41_TM=61.587
PRIMER_RIGHT_41_TM=62.711
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=44.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=10.0000
PRIMER_RIGHT_41_END_STABILITY=9.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=932
PRIMER_PAIR_42_PENALTY=11.625549
PRIMER_LEFT_42_PENALTY=3.586704
PRIMER_RIGHT_42_PENALTY=8.038845
PRIMER_LEFT_42_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_42_SEQUENCE=CACTTTATGAGACCCGTGGTTAGTG
PRIMER_LEFT_42=902,22
PRIMER_RIGHT_42=1823,25
PRIMER_LEFT_42_TM=61.587
PRIMER_RIGHT_42_TM=63.039
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=48.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.0000
PRIMER_RIGHT_42_END_STABILITY=5.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=922
PRIMER_PAIR_43_PENALTY=12.193306
PRIMER_LEFT_43_PENALTY=3.586704
PRIMER_RIGHT_43_PENALTY=8.606602
PRIMER_LEFT_43_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_43_SEQUENCE=CTTTATGAGACCCGTGGTTAGTGGT
PRIMER_LEFT_43=902,22
PRIMER_RIGHT_43=1821,25
PRIMER_LEFT_43_TM=61.587
PRIMER_RIGHT_43_TM=63.607
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=48.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.0000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=920
PRIMER_PAIR_44_PENALTY=12.193306
PRIMER_LEFT_44_PENALTY=3.586704
PRIMER_RIGHT_44_PENALTY=8.606602
PRIMER_LEFT_44_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_44_SEQUENCE=ACTTTATGAGACCCGTGGTTAGTGG
PRIMER_LEFT_44=902,22
PRIMER_RIGHT_44=1822,25
PRIMER_LEFT_44_TM=61.587
PRIMER_RIGHT_44_TM=63.607
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=48.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.0000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=921
PRIMER_PAIR_45_PENALTY=12.193306
PRIMER_LEFT_45_PENALTY=3.586704
PRIMER_RIGHT_45_PENALTY=8.606602
PRIMER_LEFT_45_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_45_SEQUENCE=CCACTTTATGAGACCCGTGGTTAGT
PRIMER_LEFT_45=902,22
PRIMER_RIGHT_45=1824,25
PRIMER_LEFT_45_TM=61.587
PRIMER_RIGHT_45_TM=63.607
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=48.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=10.0000
PRIMER_RIGHT_45_END_STABILITY=5.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=923
PRIMER_PAIR_46_PENALTY=12.483225
PRIMER_LEFT_46_PENALTY=3.586704
PRIMER_RIGHT_46_PENALTY=8.896521
PRIMER_LEFT_46_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_46_SEQUENCE=TTTATGAGACCCGTGGTTAGTGGTT
PRIMER_LEFT_46=902,22
PRIMER_RIGHT_46=1820,25
PRIMER_LEFT_46_TM=61.587
PRIMER_RIGHT_46_TM=63.897
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=44.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=10.0000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=919
PRIMER_PAIR_47_PENALTY=12.704302
PRIMER_LEFT_47_PENALTY=3.586704
PRIMER_RIGHT_47_PENALTY=9.117598
PRIMER_LEFT_47_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_47_SEQUENCE=TGAGACCCGTGGTTAGTGGTTACTT
PRIMER_LEFT_47=902,22
PRIMER_RIGHT_47=1816,25
PRIMER_LEFT_47_TM=61.587
PRIMER_RIGHT_47_TM=64.118
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=48.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=10.0000
PRIMER_RIGHT_47_END_STABILITY=5.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=915
PRIMER_PAIR_48_PENALTY=12.951484
PRIMER_LEFT_48_PENALTY=3.586704
PRIMER_RIGHT_48_PENALTY=9.364780
PRIMER_LEFT_48_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_48_SEQUENCE=CCAGAAATTGAGAAAAGCGAACTTG
PRIMER_LEFT_48=902,22
PRIMER_RIGHT_48=1869,25
PRIMER_LEFT_48_TM=61.587
PRIMER_RIGHT_48_TM=64.365
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=10.0000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=968
PRIMER_PAIR_49_PENALTY=12.996932
PRIMER_LEFT_49_PENALTY=3.586704
PRIMER_RIGHT_49_PENALTY=9.410228
PRIMER_LEFT_49_SEQUENCE=CGATTCACAAATGTTTCTTGGG
PRIMER_RIGHT_49_SEQUENCE=CTTCACAAGTCCACTTTATGAGACCC
PRIMER_LEFT_49=902,22
PRIMER_RIGHT_49=1834,26
PRIMER_LEFT_49_TM=61.587
PRIMER_RIGHT_49_TM=63.410
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=46.154
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=10.0000
PRIMER_RIGHT_49_END_STABILITY=9.1000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=933
=
SEQUENCE_ID=MAGIC.433_2_4991258_4991566_sf-2_kn-0_21684_112212_TRUE
SEQUENCE_TARGET=950,408
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGNTACTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_4515236_4515249_edi-0_ct-1_529450_6468_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_10774888_10775201_edi-0_po-0_26208_38957_TRUE
SEQUENCE_TARGET=950,413
SEQUENCE_TEMPLATE=TTAAGGGAGCACCTANTAGTGGTGTCCACGCGAAGCACCGAATCATNTTTCTTTCCTTTTTATTTTATAAGGAAAGTATAAAAATGANGGTACACATATTCTAGCTTTTGAGAGGNNNNNNNTCAGAAGTGGATGGAATTTGATTTGGATTGGGTTGGGATNACGATAGTTGCTGCCAAAACCTTTCTAGTGGAAACAAGAATAAGCTACATATTGTATTCCCTTATCAANCTTTAGTTTTGTGANTAAAGTGTTACNAAAGTGTNATAAGATAGATNAAAAAAANAAAAGAGGANAAAGTAGGANATAAAANATGATAGAATAAATTCTTGATCAAACCCCTTTACCTAGGGCTATATATNTANACNTTTAANAGCTTTTCTCTGTCAAGTTGAATGTACTGATCAAGATAACANATTNATCAAAAATAANTACATCATACTGGTTTATGAGCAAGAAACATTGCTCAGAATTATTACCANATAAGATGTTTAGAANCCAAGACTCGAAATACTTNATCCCGGTGCAGAAAGAANCGCCACCGGTGACNACTTTACCGNTGAAAGCTTCAACGGTGAANTCTCCACACAACTGTGAGGCCATTCTCAGAGACGCAGATCCTCCGATCTCTNTCTCCTCTGTTAATCTCTCNGAANAGCTACGGTNTGGTNTTTTCTTGAAACCCAAGAAACAGGTTCATATTCTTTTTAAGAATTAGCTCACTTGTGTTTCTATTTTCAGAAAANNNNNNNNNNNNNNNNNNCCTTTANGTAAACAAAATTGCAAACACNNNANTTCTNNNNNNNNAAANTTGAATCTCTNATTTNNNNNNNNNAAAGTAGCTNANANAAANNNNNNNATCACAAAACAAACGTTNCCNTTCTGCTTGGTTTAGAAAGACATGATTGCTACTTCCNCATAGGTTTCAAATNCTTCGAAAGATAANATAAGNTAGAACTAATGGACTAAAGNAACACTATCAAAATGGTAACTTTTTGNTNAGAAAATCAATCNNAATATATTTAAGACGNACCATGAATTNATTTGNTTTGNTGATCTGCNTATAATATATATTNAACAGATCAAATATTGGGTGGACGAGAGAAACAGCAACTGCTTCATGCTCTTTGCAAAGAACCTCTCTATAACTTGGTCNGATGACGTCAACTATTGGACTTGGTTTACCGANAAAGAGTCACCNAAGTAAGATACGTTTCATTTAAATCCCATACTANGAATNTTACCACAAAATGAATATCTTTGTTACATATTTGATTTACAATANAGTNGAGTATNAATCAAAGTATCCTTGANAAANTCGAAACAGGTNACGACGTTTTNAGTTTNTNNGTNTATATTATAGCAATATTAATACNANNNNTGACCTATAATTATTAATGCAAAGTNACAAACTTTAGTNTGTTTTNGATNATTTTTAAAGCGAGAATGTGGAAGCTGTGNGATTGAAAAACGTTTGTTGGCTCGACATCACGGGAAAATTCGACACGAGNAACCTCACTCCGGGGATTGTTTACGAGGTNGTCTTTAAGGTGAAGCTAGAGGATCCGGCNTATGGATGGGACACGCCGGTGAACCTAAAGCTAGTCTTGCCTNACGGTAAGGANANNCCACAAGAGNAAAAGNTGAGTTTGAGGGAACTTCCAAGGTATAAATGGGTCGATGTCAGAGTTGGCGAGTTCGTACCTGAGAAATCCGCTGCCGGNGAGATCACTTTCTCAATGNATGAGCATGNGGCTGGTGTTTGGAAGAAAGGGCTCTCCCTCAAAGGTGTTGCAATTCGTCCCAAACAGTAATAATTGTTATGGCATAGAAAAACATAAATCTCCATATGCACCNATGGACCTTCTCAACATATANNTCACATCATGTCATGTGTGTATAATAAGGTTGTTCTATCGTANNNNNNNTTTTAATAATAACTTTAATGTTGGTGTTGAATAAACTCAAAACGAGTGNNNNNNNNNNNNNNNNNNNNGAATCAATTNATTTACAATGTTCTAACTTTTAACGAGCTGACATGAATTNATTNTTGGNNANAAAANAAAAAGTAGAAAGATTTTCTATGAACCTTAAANCTANTTAGATCCTATCAAAACCCATATTTANTTTGGCNTAAACAATATTTTNTANACATGTGTAAAATATCCACATAATCTCNCAATTAGGCATTTTTGATAGATAATCTTAAAGGNATATGANCNAACAGTCTATATGCGTGGATCATGTTCACNTTTTAAAAGAGTATTCNAAAATTCCATTTTGGTGGAGAAAATGGAAGAATAAAGCTAGCTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.423037
PRIMER_LEFT_0_PENALTY=0.403270
PRIMER_RIGHT_0_PENALTY=0.019767
PRIMER_LEFT_0_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_0_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_0=584,20
PRIMER_RIGHT_0=1458,20
PRIMER_LEFT_0_TM=59.597
PRIMER_RIGHT_0_TM=60.020
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=875
PRIMER_PAIR_1_PENALTY=0.606637
PRIMER_LEFT_1_PENALTY=0.403270
PRIMER_RIGHT_1_PENALTY=0.203367
PRIMER_LEFT_1_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_1_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_1=584,20
PRIMER_RIGHT_1=1504,20
PRIMER_LEFT_1_TM=59.597
PRIMER_RIGHT_1_TM=59.797
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=921
PRIMER_PAIR_2_PENALTY=0.941354
PRIMER_LEFT_2_PENALTY=0.403270
PRIMER_RIGHT_2_PENALTY=0.538083
PRIMER_LEFT_2_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_2_SEQUENCE=CAGCTTCCACATTCTCGCTT
PRIMER_LEFT_2=584,20
PRIMER_RIGHT_2=1457,20
PRIMER_LEFT_2_TM=59.597
PRIMER_RIGHT_2_TM=60.538
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=874
PRIMER_PAIR_3_PENALTY=1.295123
PRIMER_LEFT_3_PENALTY=1.275356
PRIMER_RIGHT_3_PENALTY=0.019767
PRIMER_LEFT_3_SEQUENCE=CACAACTGTGAGGCCATTCT
PRIMER_RIGHT_3_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_3=586,20
PRIMER_RIGHT_3=1458,20
PRIMER_LEFT_3_TM=58.725
PRIMER_RIGHT_3_TM=60.020
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=873
PRIMER_PAIR_4_PENALTY=1.323230
PRIMER_LEFT_4_PENALTY=0.403270
PRIMER_RIGHT_4_PENALTY=0.919960
PRIMER_LEFT_4_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_4_SEQUENCE=AGCTTCCACATTCTCGCTTT
PRIMER_LEFT_4=584,20
PRIMER_RIGHT_4=1456,20
PRIMER_LEFT_4_TM=59.597
PRIMER_RIGHT_4_TM=59.080
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=873
PRIMER_PAIR_5_PENALTY=1.382639
PRIMER_LEFT_5_PENALTY=1.362873
PRIMER_RIGHT_5_PENALTY=0.019767
PRIMER_LEFT_5_SEQUENCE=ACACAACTGTGAGGCCATTCT
PRIMER_RIGHT_5_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_5=585,21
PRIMER_RIGHT_5=1458,20
PRIMER_LEFT_5_TM=59.637
PRIMER_RIGHT_5_TM=60.020
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=874
PRIMER_PAIR_6_PENALTY=1.451830
PRIMER_LEFT_6_PENALTY=1.432063
PRIMER_RIGHT_6_PENALTY=0.019767
PRIMER_LEFT_6_SEQUENCE=ACACAACTGTGAGGCCATTC
PRIMER_RIGHT_6_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_6=585,20
PRIMER_RIGHT_6=1458,20
PRIMER_LEFT_6_TM=58.568
PRIMER_RIGHT_6_TM=60.020
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=9.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=874
PRIMER_PAIR_7_PENALTY=1.478723
PRIMER_LEFT_7_PENALTY=1.275356
PRIMER_RIGHT_7_PENALTY=0.203367
PRIMER_LEFT_7_SEQUENCE=CACAACTGTGAGGCCATTCT
PRIMER_RIGHT_7_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_7=586,20
PRIMER_RIGHT_7=1504,20
PRIMER_LEFT_7_TM=58.725
PRIMER_RIGHT_7_TM=59.797
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=6.3000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=919
PRIMER_PAIR_8_PENALTY=1.517945
PRIMER_LEFT_8_PENALTY=0.403270
PRIMER_RIGHT_8_PENALTY=1.114674
PRIMER_LEFT_8_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_8_SEQUENCE=CCGGATCCTCTAGCTTCACC
PRIMER_LEFT_8=584,20
PRIMER_RIGHT_8=1568,20
PRIMER_LEFT_8_TM=59.597
PRIMER_RIGHT_8_TM=61.115
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=60.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=985
PRIMER_PAIR_9_PENALTY=1.547021
PRIMER_LEFT_9_PENALTY=0.403270
PRIMER_RIGHT_9_PENALTY=1.143751
PRIMER_LEFT_9_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_9_SEQUENCE=CGGATCCTCTAGCTTCACCTT
PRIMER_LEFT_9=584,20
PRIMER_RIGHT_9=1567,21
PRIMER_LEFT_9_TM=59.597
PRIMER_RIGHT_9_TM=59.856
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=984
PRIMER_PAIR_10_PENALTY=1.566239
PRIMER_LEFT_10_PENALTY=1.362873
PRIMER_RIGHT_10_PENALTY=0.203367
PRIMER_LEFT_10_SEQUENCE=ACACAACTGTGAGGCCATTCT
PRIMER_RIGHT_10_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_10=585,21
PRIMER_RIGHT_10=1504,20
PRIMER_LEFT_10_TM=59.637
PRIMER_RIGHT_10_TM=59.797
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=920
PRIMER_PAIR_11_PENALTY=1.635430
PRIMER_LEFT_11_PENALTY=1.432063
PRIMER_RIGHT_11_PENALTY=0.203367
PRIMER_LEFT_11_SEQUENCE=ACACAACTGTGAGGCCATTC
PRIMER_RIGHT_11_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_11=585,20
PRIMER_RIGHT_11=1504,20
PRIMER_LEFT_11_TM=58.568
PRIMER_RIGHT_11_TM=59.797
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=920
PRIMER_PAIR_12_PENALTY=1.664287
PRIMER_LEFT_12_PENALTY=0.403270
PRIMER_RIGHT_12_PENALTY=1.261017
PRIMER_LEFT_12_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_12_SEQUENCE=GCCGGATCCTCTAGCTTCAC
PRIMER_LEFT_12=584,20
PRIMER_RIGHT_12=1569,20
PRIMER_LEFT_12_TM=59.597
PRIMER_RIGHT_12_TM=61.261
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=60.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=986
PRIMER_PAIR_13_PENALTY=1.725309
PRIMER_LEFT_13_PENALTY=1.705543
PRIMER_RIGHT_13_PENALTY=0.019767
PRIMER_LEFT_13_SEQUENCE=CACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_13_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_13=586,21
PRIMER_RIGHT_13=1458,20
PRIMER_LEFT_13_TM=60.706
PRIMER_RIGHT_13_TM=60.020
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=873
PRIMER_PAIR_14_PENALTY=1.813439
PRIMER_LEFT_14_PENALTY=1.275356
PRIMER_RIGHT_14_PENALTY=0.538083
PRIMER_LEFT_14_SEQUENCE=CACAACTGTGAGGCCATTCT
PRIMER_RIGHT_14_SEQUENCE=CAGCTTCCACATTCTCGCTT
PRIMER_LEFT_14=586,20
PRIMER_RIGHT_14=1457,20
PRIMER_LEFT_14_TM=58.725
PRIMER_RIGHT_14_TM=60.538
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=10.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=872
PRIMER_PAIR_15_PENALTY=1.819020
PRIMER_LEFT_15_PENALTY=1.799253
PRIMER_RIGHT_15_PENALTY=0.019767
PRIMER_LEFT_15_SEQUENCE=TCTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_15_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_15=674,21
PRIMER_RIGHT_15=1458,20
PRIMER_LEFT_15_TM=59.201
PRIMER_RIGHT_15_TM=60.020
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=785
PRIMER_PAIR_16_PENALTY=1.900956
PRIMER_LEFT_16_PENALTY=1.362873
PRIMER_RIGHT_16_PENALTY=0.538083
PRIMER_LEFT_16_SEQUENCE=ACACAACTGTGAGGCCATTCT
PRIMER_RIGHT_16_SEQUENCE=CAGCTTCCACATTCTCGCTT
PRIMER_LEFT_16=585,21
PRIMER_RIGHT_16=1457,20
PRIMER_LEFT_16_TM=59.637
PRIMER_RIGHT_16_TM=60.538
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=10.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=873
PRIMER_PAIR_17_PENALTY=1.908909
PRIMER_LEFT_17_PENALTY=1.705543
PRIMER_RIGHT_17_PENALTY=0.203367
PRIMER_LEFT_17_SEQUENCE=CACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_17_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_17=586,21
PRIMER_RIGHT_17=1504,20
PRIMER_LEFT_17_TM=60.706
PRIMER_RIGHT_17_TM=59.797
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.3000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=919
PRIMER_PAIR_18_PENALTY=1.970147
PRIMER_LEFT_18_PENALTY=1.432063
PRIMER_RIGHT_18_PENALTY=0.538083
PRIMER_LEFT_18_SEQUENCE=ACACAACTGTGAGGCCATTC
PRIMER_RIGHT_18_SEQUENCE=CAGCTTCCACATTCTCGCTT
PRIMER_LEFT_18=585,20
PRIMER_RIGHT_18=1457,20
PRIMER_LEFT_18_TM=58.568
PRIMER_RIGHT_18_TM=60.538
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=10.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=873
PRIMER_PAIR_19_PENALTY=2.002620
PRIMER_LEFT_19_PENALTY=1.799253
PRIMER_RIGHT_19_PENALTY=0.203367
PRIMER_LEFT_19_SEQUENCE=TCTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_19_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_19=674,21
PRIMER_RIGHT_19=1504,20
PRIMER_LEFT_19_TM=59.201
PRIMER_RIGHT_19_TM=59.797
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=831
PRIMER_PAIR_20_PENALTY=2.195316
PRIMER_LEFT_20_PENALTY=1.275356
PRIMER_RIGHT_20_PENALTY=0.919960
PRIMER_LEFT_20_SEQUENCE=CACAACTGTGAGGCCATTCT
PRIMER_RIGHT_20_SEQUENCE=AGCTTCCACATTCTCGCTTT
PRIMER_LEFT_20=586,20
PRIMER_RIGHT_20=1456,20
PRIMER_LEFT_20_TM=58.725
PRIMER_RIGHT_20_TM=59.080
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=871
PRIMER_PAIR_21_PENALTY=2.214605
PRIMER_LEFT_21_PENALTY=0.403270
PRIMER_RIGHT_21_PENALTY=1.811335
PRIMER_LEFT_21_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_21_SEQUENCE=TGTCGAATTTTCCCGTGATG
PRIMER_LEFT_21=584,20
PRIMER_RIGHT_21=1505,20
PRIMER_LEFT_21_TM=59.597
PRIMER_RIGHT_21_TM=61.811
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=922
PRIMER_PAIR_22_PENALTY=2.243626
PRIMER_LEFT_22_PENALTY=1.705543
PRIMER_RIGHT_22_PENALTY=0.538083
PRIMER_LEFT_22_SEQUENCE=CACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_22_SEQUENCE=CAGCTTCCACATTCTCGCTT
PRIMER_LEFT_22=586,21
PRIMER_RIGHT_22=1457,20
PRIMER_LEFT_22_TM=60.706
PRIMER_RIGHT_22_TM=60.538
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=10.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=872
PRIMER_PAIR_23_PENALTY=2.282832
PRIMER_LEFT_23_PENALTY=1.362873
PRIMER_RIGHT_23_PENALTY=0.919960
PRIMER_LEFT_23_SEQUENCE=ACACAACTGTGAGGCCATTCT
PRIMER_RIGHT_23_SEQUENCE=AGCTTCCACATTCTCGCTTT
PRIMER_LEFT_23=585,21
PRIMER_RIGHT_23=1456,20
PRIMER_LEFT_23_TM=59.637
PRIMER_RIGHT_23_TM=59.080
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=872
PRIMER_PAIR_24_PENALTY=2.312214
PRIMER_LEFT_24_PENALTY=2.292447
PRIMER_RIGHT_24_PENALTY=0.019767
PRIMER_LEFT_24_SEQUENCE=ACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_24_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_24=587,20
PRIMER_RIGHT_24=1458,20
PRIMER_LEFT_24_TM=57.708
PRIMER_RIGHT_24_TM=60.020
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=9.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=872
PRIMER_PAIR_25_PENALTY=2.337337
PRIMER_LEFT_25_PENALTY=1.799253
PRIMER_RIGHT_25_PENALTY=0.538083
PRIMER_LEFT_25_SEQUENCE=TCTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_25_SEQUENCE=CAGCTTCCACATTCTCGCTT
PRIMER_LEFT_25=674,21
PRIMER_RIGHT_25=1457,20
PRIMER_LEFT_25_TM=59.201
PRIMER_RIGHT_25_TM=60.538
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=10.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=784
PRIMER_PAIR_26_PENALTY=2.352023
PRIMER_LEFT_26_PENALTY=1.432063
PRIMER_RIGHT_26_PENALTY=0.919960
PRIMER_LEFT_26_SEQUENCE=ACACAACTGTGAGGCCATTC
PRIMER_RIGHT_26_SEQUENCE=AGCTTCCACATTCTCGCTTT
PRIMER_LEFT_26=585,20
PRIMER_RIGHT_26=1456,20
PRIMER_LEFT_26_TM=58.568
PRIMER_RIGHT_26_TM=59.080
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.9000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=872
PRIMER_PAIR_27_PENALTY=2.390030
PRIMER_LEFT_27_PENALTY=1.275356
PRIMER_RIGHT_27_PENALTY=1.114674
PRIMER_LEFT_27_SEQUENCE=CACAACTGTGAGGCCATTCT
PRIMER_RIGHT_27_SEQUENCE=CCGGATCCTCTAGCTTCACC
PRIMER_LEFT_27=586,20
PRIMER_RIGHT_27=1568,20
PRIMER_LEFT_27_TM=58.725
PRIMER_RIGHT_27_TM=61.115
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=60.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.6000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=983
PRIMER_PAIR_28_PENALTY=2.419107
PRIMER_LEFT_28_PENALTY=1.275356
PRIMER_RIGHT_28_PENALTY=1.143751
PRIMER_LEFT_28_SEQUENCE=CACAACTGTGAGGCCATTCT
PRIMER_RIGHT_28_SEQUENCE=CGGATCCTCTAGCTTCACCTT
PRIMER_LEFT_28=586,20
PRIMER_RIGHT_28=1567,21
PRIMER_LEFT_28_TM=58.725
PRIMER_RIGHT_28_TM=59.856
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=982
PRIMER_PAIR_29_PENALTY=2.477547
PRIMER_LEFT_29_PENALTY=1.362873
PRIMER_RIGHT_29_PENALTY=1.114674
PRIMER_LEFT_29_SEQUENCE=ACACAACTGTGAGGCCATTCT
PRIMER_RIGHT_29_SEQUENCE=CCGGATCCTCTAGCTTCACC
PRIMER_LEFT_29=585,21
PRIMER_RIGHT_29=1568,20
PRIMER_LEFT_29_TM=59.637
PRIMER_RIGHT_29_TM=61.115
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=60.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=984
PRIMER_PAIR_30_PENALTY=2.495814
PRIMER_LEFT_30_PENALTY=2.292447
PRIMER_RIGHT_30_PENALTY=0.203367
PRIMER_LEFT_30_SEQUENCE=ACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_30_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_30=587,20
PRIMER_RIGHT_30=1504,20
PRIMER_LEFT_30_TM=57.708
PRIMER_RIGHT_30_TM=59.797
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.3000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=918
PRIMER_PAIR_31_PENALTY=2.506624
PRIMER_LEFT_31_PENALTY=1.362873
PRIMER_RIGHT_31_PENALTY=1.143751
PRIMER_LEFT_31_SEQUENCE=ACACAACTGTGAGGCCATTCT
PRIMER_RIGHT_31_SEQUENCE=CGGATCCTCTAGCTTCACCTT
PRIMER_LEFT_31=585,21
PRIMER_RIGHT_31=1567,21
PRIMER_LEFT_31_TM=59.637
PRIMER_RIGHT_31_TM=59.856
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=983
PRIMER_PAIR_32_PENALTY=2.536373
PRIMER_LEFT_32_PENALTY=1.275356
PRIMER_RIGHT_32_PENALTY=1.261017
PRIMER_LEFT_32_SEQUENCE=CACAACTGTGAGGCCATTCT
PRIMER_RIGHT_32_SEQUENCE=GCCGGATCCTCTAGCTTCAC
PRIMER_LEFT_32=586,20
PRIMER_RIGHT_32=1569,20
PRIMER_LEFT_32_TM=58.725
PRIMER_RIGHT_32_TM=61.261
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=60.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=984
PRIMER_PAIR_33_PENALTY=2.546738
PRIMER_LEFT_33_PENALTY=1.432063
PRIMER_RIGHT_33_PENALTY=1.114674
PRIMER_LEFT_33_SEQUENCE=ACACAACTGTGAGGCCATTC
PRIMER_RIGHT_33_SEQUENCE=CCGGATCCTCTAGCTTCACC
PRIMER_LEFT_33=585,20
PRIMER_RIGHT_33=1568,20
PRIMER_LEFT_33_TM=58.568
PRIMER_RIGHT_33_TM=61.115
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=60.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=984
PRIMER_PAIR_34_PENALTY=2.575815
PRIMER_LEFT_34_PENALTY=1.432063
PRIMER_RIGHT_34_PENALTY=1.143751
PRIMER_LEFT_34_SEQUENCE=ACACAACTGTGAGGCCATTC
PRIMER_RIGHT_34_SEQUENCE=CGGATCCTCTAGCTTCACCTT
PRIMER_LEFT_34=585,20
PRIMER_RIGHT_34=1567,21
PRIMER_LEFT_34_TM=58.568
PRIMER_RIGHT_34_TM=59.856
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=983
PRIMER_PAIR_35_PENALTY=2.588574
PRIMER_LEFT_35_PENALTY=2.568807
PRIMER_RIGHT_35_PENALTY=0.019767
PRIMER_LEFT_35_SEQUENCE=CACACAACTGTGAGGCCATTC
PRIMER_RIGHT_35_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_35=584,21
PRIMER_RIGHT_35=1458,20
PRIMER_LEFT_35_TM=61.569
PRIMER_RIGHT_35_TM=60.020
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=875
PRIMER_PAIR_36_PENALTY=2.623889
PRIMER_LEFT_36_PENALTY=1.362873
PRIMER_RIGHT_36_PENALTY=1.261017
PRIMER_LEFT_36_SEQUENCE=ACACAACTGTGAGGCCATTCT
PRIMER_RIGHT_36_SEQUENCE=GCCGGATCCTCTAGCTTCAC
PRIMER_LEFT_36=585,21
PRIMER_RIGHT_36=1569,20
PRIMER_LEFT_36_TM=59.637
PRIMER_RIGHT_36_TM=61.261
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=60.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=985
PRIMER_PAIR_37_PENALTY=2.625502
PRIMER_LEFT_37_PENALTY=1.705543
PRIMER_RIGHT_37_PENALTY=0.919960
PRIMER_LEFT_37_SEQUENCE=CACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_37_SEQUENCE=AGCTTCCACATTCTCGCTTT
PRIMER_LEFT_37=586,21
PRIMER_RIGHT_37=1456,20
PRIMER_LEFT_37_TM=60.706
PRIMER_RIGHT_37_TM=59.080
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=871
PRIMER_PAIR_38_PENALTY=2.693080
PRIMER_LEFT_38_PENALTY=1.432063
PRIMER_RIGHT_38_PENALTY=1.261017
PRIMER_LEFT_38_SEQUENCE=ACACAACTGTGAGGCCATTC
PRIMER_RIGHT_38_SEQUENCE=GCCGGATCCTCTAGCTTCAC
PRIMER_LEFT_38=585,20
PRIMER_RIGHT_38=1569,20
PRIMER_LEFT_38_TM=58.568
PRIMER_RIGHT_38_TM=61.261
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=60.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=985
PRIMER_PAIR_39_PENALTY=2.714713
PRIMER_LEFT_39_PENALTY=2.694946
PRIMER_RIGHT_39_PENALTY=0.019767
PRIMER_LEFT_39_SEQUENCE=CTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_39_SEQUENCE=ACAGCTTCCACATTCTCGCT
PRIMER_LEFT_39=675,20
PRIMER_RIGHT_39=1458,20
PRIMER_LEFT_39_TM=57.305
PRIMER_RIGHT_39_TM=60.020
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=9.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=784
PRIMER_PAIR_40_PENALTY=2.719213
PRIMER_LEFT_40_PENALTY=1.799253
PRIMER_RIGHT_40_PENALTY=0.919960
PRIMER_LEFT_40_SEQUENCE=TCTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_40_SEQUENCE=AGCTTCCACATTCTCGCTTT
PRIMER_LEFT_40=674,21
PRIMER_RIGHT_40=1456,20
PRIMER_LEFT_40_TM=59.201
PRIMER_RIGHT_40_TM=59.080
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=783
PRIMER_PAIR_41_PENALTY=2.719625
PRIMER_LEFT_41_PENALTY=0.403270
PRIMER_RIGHT_41_PENALTY=2.316355
PRIMER_LEFT_41_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_41_SEQUENCE=CTCGTGTCGAATTTTCCCGT
PRIMER_LEFT_41=584,20
PRIMER_RIGHT_41=1509,20
PRIMER_LEFT_41_TM=59.597
PRIMER_RIGHT_41_TM=62.316
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.4000
PRIMER_RIGHT_41_END_STABILITY=11.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=926
PRIMER_PAIR_42_PENALTY=2.731166
PRIMER_LEFT_42_PENALTY=0.403270
PRIMER_RIGHT_42_PENALTY=2.327896
PRIMER_LEFT_42_SEQUENCE=CACACAACTGTGAGGCCATT
PRIMER_RIGHT_42_SEQUENCE=ACAGCTTCCACATTCTCGCTT
PRIMER_LEFT_42=584,20
PRIMER_RIGHT_42=1458,21
PRIMER_LEFT_42_TM=59.597
PRIMER_RIGHT_42_TM=61.328
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=10.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=875
PRIMER_PAIR_43_PENALTY=2.772174
PRIMER_LEFT_43_PENALTY=2.568807
PRIMER_RIGHT_43_PENALTY=0.203367
PRIMER_LEFT_43_SEQUENCE=CACACAACTGTGAGGCCATTC
PRIMER_RIGHT_43_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_43=584,21
PRIMER_RIGHT_43=1504,20
PRIMER_LEFT_43_TM=61.569
PRIMER_RIGHT_43_TM=59.797
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=921
PRIMER_PAIR_44_PENALTY=2.820217
PRIMER_LEFT_44_PENALTY=1.705543
PRIMER_RIGHT_44_PENALTY=1.114674
PRIMER_LEFT_44_SEQUENCE=CACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_44_SEQUENCE=CCGGATCCTCTAGCTTCACC
PRIMER_LEFT_44=586,21
PRIMER_RIGHT_44=1568,20
PRIMER_LEFT_44_TM=60.706
PRIMER_RIGHT_44_TM=61.115
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=60.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=983
PRIMER_PAIR_45_PENALTY=2.830530
PRIMER_LEFT_45_PENALTY=2.292447
PRIMER_RIGHT_45_PENALTY=0.538083
PRIMER_LEFT_45_SEQUENCE=ACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_45_SEQUENCE=CAGCTTCCACATTCTCGCTT
PRIMER_LEFT_45=587,20
PRIMER_RIGHT_45=1457,20
PRIMER_LEFT_45_TM=57.708
PRIMER_RIGHT_45_TM=60.538
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=10.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=871
PRIMER_PAIR_46_PENALTY=2.849294
PRIMER_LEFT_46_PENALTY=1.705543
PRIMER_RIGHT_46_PENALTY=1.143751
PRIMER_LEFT_46_SEQUENCE=CACAACTGTGAGGCCATTCTC
PRIMER_RIGHT_46_SEQUENCE=CGGATCCTCTAGCTTCACCTT
PRIMER_LEFT_46=586,21
PRIMER_RIGHT_46=1567,21
PRIMER_LEFT_46_TM=60.706
PRIMER_RIGHT_46_TM=59.856
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=982
PRIMER_PAIR_47_PENALTY=2.898313
PRIMER_LEFT_47_PENALTY=2.694946
PRIMER_RIGHT_47_PENALTY=0.203367
PRIMER_LEFT_47_SEQUENCE=CTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_47_SEQUENCE=GTCGAATTTTCCCGTGATGT
PRIMER_LEFT_47=675,20
PRIMER_RIGHT_47=1504,20
PRIMER_LEFT_47_TM=57.305
PRIMER_RIGHT_47_TM=59.797
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=830
PRIMER_PAIR_48_PENALTY=2.913928
PRIMER_LEFT_48_PENALTY=1.799253
PRIMER_RIGHT_48_PENALTY=1.114674
PRIMER_LEFT_48_SEQUENCE=TCTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_48_SEQUENCE=CCGGATCCTCTAGCTTCACC
PRIMER_LEFT_48=674,21
PRIMER_RIGHT_48=1568,20
PRIMER_LEFT_48_TM=59.201
PRIMER_RIGHT_48_TM=61.115
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=60.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=895
PRIMER_PAIR_49_PENALTY=2.943005
PRIMER_LEFT_49_PENALTY=1.799253
PRIMER_RIGHT_49_PENALTY=1.143751
PRIMER_LEFT_49_SEQUENCE=TCTTGAAACCCAAGAAACAGG
PRIMER_RIGHT_49_SEQUENCE=CGGATCCTCTAGCTTCACCTT
PRIMER_LEFT_49=674,21
PRIMER_RIGHT_49=1567,21
PRIMER_LEFT_49_TM=59.201
PRIMER_RIGHT_49_TM=59.856
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=894
=
SEQUENCE_ID=MAGIC.433_4_1867713_1868029_wu-0_oy-0_240492_353475_TRUE
SEQUENCE_TARGET=950,416
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCATTNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTCCCATGGGATGGCGAGTTTNCGCTCTCCACATTGTATTATGGAGAGTATGNNAANNNTGGTCCTGGAGCGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAAAATAATAGTTTCATATTAAATTTTACACGTTGGTCATATAGGGGNTCCTTGCNTANTGCATTCTGNATGGCNTGTCAGCACAGTGNATCAGCATGTGATATAATTTTACNNTAAGTAGAGCATTCATCGGNCGTNAATTTTNGNAATATTCAGTGTATTTATTTTCTNTTTTTTTCNGTTTTCTATTATTNNTGTATTTCTCAATACAATNNAAATTCAAACTAACCNAAAATTCAGAAAAGATCACTATATTATATATAAAATCAATTGCCGCCNAAAAAGAAATCTGTTTTACAATGAATATAANTGAACAATTATACATATATCTAGCAAATCTATCTNTTGGCATTTNTAAANTANTAATCTCCATTGAAGAGCTGCAACTTTGTGCTTGCTAAAACTTTTTCACNTGCAACAAAATGAGTATTATGGGAGATTTAAAAATCAACATAGGCATANAAAAATATTCCAGAAANANNAGAGAGACAATAAGGAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17500033_17500211_ct-1_po-0_17886_41010_TRUE
SEQUENCE_TARGET=950,278
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCGCTGTGTTTNTTCTTCTTTCTTACAANCTCTCCATTTCTCTCTCTTCCGTCTCTTTTNTNATCTNTCTCTCTAGAATCTTGAAGAGAGCGAGCTTCCATTAAANTCTCATTCTAACGATCTNCAAGCTCTTTCTTCAATCATCTCCAGGTTTGCTTCGATCTACACGGCGTCGTTTACACGTCTCCTTCNAATCTTTCNTCCTTACTTCTCCGATTTCAATTTNNTGCCNTTTNTNTNNNNAGTTTTNCGATCGCTGATTTCTTCAAATATTTATCNNANTTTTTNTCNATGNTTTCTNTTTGTAAATTCTNTGTAATTCAATTAATTGAATCGAATANTTTTGTCTCTCTTGCATTTCCTCAGCTCCAGCTTTTGTTTTTATTAGTTTCTAAGATTGCTTTTTGTTTTTAGGGTTTATTCTCAATTCTTATATAGTTTTTGAGNTTTTTGNTNTATTGATTTGATTCATGTGAGTGANTGATTGATTTAGAATCTTTNTTNAGCTGTGACTGTGATTTAGATTTGATNCACTTCAGCTNTAATTCTTTGTTTTGATGATGATTATGATNAATTCGTAATTTTGAGCTTATTTGAATTCATTAACTAATTTAGTATGATGAAATCTTAATCAGGGGAATGTCAGAGAGTACTAATGAAGATAGTGGAGCAGGAAGATCATCANTGGAAGAGAATTCAAATGGACAAAGAAGTCAATCAGAAGAAGCAATCGCNGAGTGGCGNTCNTCTGAACAAGTTGAGAATGGAACTCCTTCTACATCTCCACCGTATTGGGACATCGANGAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_22140861_22140966_bur-0_oy-0_32732_46615_TRUE
SEQUENCE_TARGET=950,205
SEQUENCE_TEMPLATE=CTAAGCAATCAGCATTTGAACCTGTAAACGGCTCCGGCCCGCACTCGCCTAAGAACGTGAAGCATGCAGCATCGCTCTTCAGGATTTCTACATTTTTACATTTCATTAAATTCATCANCAAAAGNTTATTATGTAANTGNAANTTTTTGCATATACATATNNACACAAAGTATAATAGAGATTANAGAATATATTCGTACCGGTGGAAGCCACCAAAAGAACCAGNAAGAGAACGGTGAAGCTAACGGAGTTGAGGTTCTTTGCCATTTCTTTAATGCTTTGCGTTTAGTAAGACTTCTTTTTTTGCTCTTTCAATTTAATAAACTTTACTTCGATGAGACAAATCCANATGTGTAGTGATCTATTTATAGTCAAAANTTTGGTAGATTTGTTGTAATTGTTTAATCTCTCCCTCTTTTCTTTAAAGTTTTCCCTTATTGANCAAGTTNATAATGTCGCCACAATTGTGTATTGCCGCCAGTTTTTATAACATTTTATTTTCCATTAGTGGGATCTTCTTCTTATCACGGACTTGTTTTAAATAATCAANCAAAGNGAAAGTCGTTGTTGTCTTTATCGTTGTAACTCATTTTGTATACCCATTTTTAGTTTAGTTTAATCAACTATTGTTTGAAAAATGATAAAGTTGTTGTCTTTGACAGAGTGAGCACGAATCTTGGCAACTTTTTAATCATAAAATAAAAATGATTTGGCTTCATTAGTTCATTAATAAAGTTNAGGGTTTCAGCAGCATTGCAGTTATCATGGCGACACTATCTCGTTTTTTTAGCATTTNCGTAGTATGTGTTAACTTCTATGCATGTCAAAACGAAATAGNAAGGGATTTGTNTTCACAGAACCTANAAAATCTCGTNTGCCTTTTATCTTAAGTTTCANATTNNTTTTATTTANTTNTCTGGCAAGGTAANCTAATCACCTATTTTATCTNTTTTGGAGGAATACTTACGTTTCTAAAAATTCTTATTTTGTTGGATTAATTNCTACTACCATNGTTTTGTTTGCTAAATAGTATGTATAATCTTAATTGGGAAAAGCCAAAAGGCATGAAAGATTTGAGGCGTAGGANTACGCCTTCGTTTGTTTCNAGCCTTAAAGNCGAAAAATACGAAAAAGAGATATCGATGTTCTTCTAGAAAGCACAGCTCATTTCTCGTTTATTTATTTATTATTCTANTTTTGAATACAATCATTTTCTTATAATACGTTCATATTCCGAATGATAACAAAGATCTCTTGTCATGCTAGACTCCTTAAAAAAAGGAAGAAGGTAAAACTGAAATTTATCAGTGTTAAACACTATAACAACATGTGCACGTTTTCATTTCTTTTTTCGATTTAGTAATATTTCGAGAAATAAATCCAGTTTCTTCAACGAACTAAAATAGAAATATACTACTAATTTGGGTTATGTTCTTTCAAAATGTTATGAATATCAACTGTTTTCCTCATCCGTCTTAAACAACGAGATNAAGACAAGACCTGATTTTCCCTCNAACGTTGATATAACATTTTTTCTTATATGNCAAACATATATCAAATAAATTAGGGAATTAATTTACTAATAAATGTTAACACACTAAANAATTGCGAAAAAATACTAGTCATAAACAGAAAAGCGAATGAAATGATAAACTAATATATAAATCGNAAAACATAATGTAAGCTTAATTTGTTAATAAGCTTGGAAAATAATAAGTAATTGTACATGAACCATGTTTAAAACAGGGCACGAANCCGTTTCAATNTACGTTTGTAAATCTTTATCAGAAAAACAAAAAAAAAGATACGTACGTTTGTAATAAGCACGTTTATAAGAGACGTACGTATCNTAATGGAATNNNTCATCAGGGAGGAGAAATAAAGAACAGGTTGGCAGATGGTAGGAGTTAGGGATTGGCTGAAAAGGANCATGTCTTTTAATGTTGTCACACCTTATGATCCCACCACACTCGTTATTCAATTGCATTATNTTGGACATTATTCATTGCATTTTGGATAATGTTCATTGCCACTTCATCCCCGCATCGTTTGGGTTTGTTTCGTTTTGGCTCAATCAGTTTTCTTAGAGCAAAGTTATACTTAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.787864
PRIMER_LEFT_0_PENALTY=0.405717
PRIMER_RIGHT_0_PENALTY=1.382147
PRIMER_LEFT_0_SEQUENCE=GAGCACGAATCTTGGCAACT
PRIMER_RIGHT_0_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_0=665,20
PRIMER_RIGHT_0=1484,21
PRIMER_LEFT_0_TM=60.406
PRIMER_RIGHT_0_TM=59.618
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=820
PRIMER_PAIR_1_PENALTY=1.864207
PRIMER_LEFT_1_PENALTY=0.003166
PRIMER_RIGHT_1_PENALTY=1.861041
PRIMER_LEFT_1_SEQUENCE=GTGTATTGCCGCCAGTTTTT
PRIMER_RIGHT_1_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_1=466,20
PRIMER_RIGHT_1=1175,21
PRIMER_LEFT_1_TM=60.003
PRIMER_RIGHT_1_TM=59.139
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=710
PRIMER_PAIR_2_PENALTY=1.985696
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=1.861041
PRIMER_LEFT_2_SEQUENCE=GAACGGTGAAGCTAACGGAG
PRIMER_RIGHT_2_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_2=230,20
PRIMER_RIGHT_2=1175,21
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=59.139
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=946
PRIMER_PAIR_3_PENALTY=1.985696
PRIMER_LEFT_3_PENALTY=0.124655
PRIMER_RIGHT_3_PENALTY=1.861041
PRIMER_LEFT_3_SEQUENCE=AGAACGGTGAAGCTAACGGA
PRIMER_RIGHT_3_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_3=229,20
PRIMER_RIGHT_3=1175,21
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.139
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=947
PRIMER_PAIR_4_PENALTY=2.266758
PRIMER_LEFT_4_PENALTY=0.405717
PRIMER_RIGHT_4_PENALTY=1.861041
PRIMER_LEFT_4_SEQUENCE=GAGCACGAATCTTGGCAACT
PRIMER_RIGHT_4_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_4=665,20
PRIMER_RIGHT_4=1175,21
PRIMER_LEFT_4_TM=60.406
PRIMER_RIGHT_4_TM=59.139
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=511
PRIMER_PAIR_5_PENALTY=2.305182
PRIMER_LEFT_5_PENALTY=0.003166
PRIMER_RIGHT_5_PENALTY=2.302015
PRIMER_LEFT_5_SEQUENCE=GTGTATTGCCGCCAGTTTTT
PRIMER_RIGHT_5_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_5=466,20
PRIMER_RIGHT_5=1174,20
PRIMER_LEFT_5_TM=60.003
PRIMER_RIGHT_5_TM=57.698
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=709
PRIMER_PAIR_6_PENALTY=2.426671
PRIMER_LEFT_6_PENALTY=0.124655
PRIMER_RIGHT_6_PENALTY=2.302015
PRIMER_LEFT_6_SEQUENCE=GAACGGTGAAGCTAACGGAG
PRIMER_RIGHT_6_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_6=230,20
PRIMER_RIGHT_6=1174,20
PRIMER_LEFT_6_TM=59.875
PRIMER_RIGHT_6_TM=57.698
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=945
PRIMER_PAIR_7_PENALTY=2.426671
PRIMER_LEFT_7_PENALTY=0.124655
PRIMER_RIGHT_7_PENALTY=2.302015
PRIMER_LEFT_7_SEQUENCE=AGAACGGTGAAGCTAACGGA
PRIMER_RIGHT_7_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_7=229,20
PRIMER_RIGHT_7=1174,20
PRIMER_LEFT_7_TM=59.875
PRIMER_RIGHT_7_TM=57.698
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=946
PRIMER_PAIR_8_PENALTY=2.435641
PRIMER_LEFT_8_PENALTY=0.003166
PRIMER_RIGHT_8_PENALTY=2.432475
PRIMER_LEFT_8_SEQUENCE=GTGTATTGCCGCCAGTTTTT
PRIMER_RIGHT_8_SEQUENCE=AAACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_8=466,20
PRIMER_RIGHT_8=1176,22
PRIMER_LEFT_8_TM=60.003
PRIMER_RIGHT_8_TM=60.432
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=711
PRIMER_PAIR_9_PENALTY=2.544454
PRIMER_LEFT_9_PENALTY=1.239452
PRIMER_RIGHT_9_PENALTY=1.305002
PRIMER_LEFT_9_SEQUENCE=TGGCGACACTATCTCGTTTTT
PRIMER_RIGHT_9_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_9=765,21
PRIMER_RIGHT_9=1472,20
PRIMER_LEFT_9_TM=59.761
PRIMER_RIGHT_9_TM=58.695
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=708
PRIMER_PAIR_10_PENALTY=2.544521
PRIMER_LEFT_10_PENALTY=1.239519
PRIMER_RIGHT_10_PENALTY=1.305002
PRIMER_LEFT_10_SEQUENCE=TCTTTGACAGAGTGAGCACGA
PRIMER_RIGHT_10_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_10=652,21
PRIMER_RIGHT_10=1472,20
PRIMER_LEFT_10_TM=59.760
PRIMER_RIGHT_10_TM=58.695
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=821
PRIMER_PAIR_11_PENALTY=2.554214
PRIMER_LEFT_11_PENALTY=1.249212
PRIMER_RIGHT_11_PENALTY=1.305002
PRIMER_LEFT_11_SEQUENCE=CATGGCGACACTATCTCGTT
PRIMER_RIGHT_11_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_11=763,20
PRIMER_RIGHT_11=1472,20
PRIMER_LEFT_11_TM=58.751
PRIMER_RIGHT_11_TM=58.695
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=710
PRIMER_PAIR_12_PENALTY=2.557130
PRIMER_LEFT_12_PENALTY=0.124655
PRIMER_RIGHT_12_PENALTY=2.432475
PRIMER_LEFT_12_SEQUENCE=GAACGGTGAAGCTAACGGAG
PRIMER_RIGHT_12_SEQUENCE=AAACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_12=230,20
PRIMER_RIGHT_12=1176,22
PRIMER_LEFT_12_TM=59.875
PRIMER_RIGHT_12_TM=60.432
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=947
PRIMER_PAIR_13_PENALTY=2.557130
PRIMER_LEFT_13_PENALTY=0.124655
PRIMER_RIGHT_13_PENALTY=2.432475
PRIMER_LEFT_13_SEQUENCE=AGAACGGTGAAGCTAACGGA
PRIMER_RIGHT_13_SEQUENCE=AAACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_13=229,20
PRIMER_RIGHT_13=1176,22
PRIMER_LEFT_13_TM=59.875
PRIMER_RIGHT_13_TM=60.432
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=948
PRIMER_PAIR_14_PENALTY=2.621599
PRIMER_LEFT_14_PENALTY=1.239452
PRIMER_RIGHT_14_PENALTY=1.382147
PRIMER_LEFT_14_SEQUENCE=TGGCGACACTATCTCGTTTTT
PRIMER_RIGHT_14_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_14=765,21
PRIMER_RIGHT_14=1484,21
PRIMER_LEFT_14_TM=59.761
PRIMER_RIGHT_14_TM=59.618
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=720
PRIMER_PAIR_15_PENALTY=2.621665
PRIMER_LEFT_15_PENALTY=1.239519
PRIMER_RIGHT_15_PENALTY=1.382147
PRIMER_LEFT_15_SEQUENCE=TCTTTGACAGAGTGAGCACGA
PRIMER_RIGHT_15_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_15=652,21
PRIMER_RIGHT_15=1484,21
PRIMER_LEFT_15_TM=59.760
PRIMER_RIGHT_15_TM=59.618
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=833
PRIMER_PAIR_16_PENALTY=2.631359
PRIMER_LEFT_16_PENALTY=1.249212
PRIMER_RIGHT_16_PENALTY=1.382147
PRIMER_LEFT_16_SEQUENCE=CATGGCGACACTATCTCGTT
PRIMER_RIGHT_16_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_16=763,20
PRIMER_RIGHT_16=1484,21
PRIMER_LEFT_16_TM=58.751
PRIMER_RIGHT_16_TM=59.618
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=722
PRIMER_PAIR_17_PENALTY=2.656436
PRIMER_LEFT_17_PENALTY=1.239452
PRIMER_RIGHT_17_PENALTY=1.416984
PRIMER_LEFT_17_SEQUENCE=TGGCGACACTATCTCGTTTTT
PRIMER_RIGHT_17_SEQUENCE=ACGGATGAGGAAAACAGTTGA
PRIMER_LEFT_17=765,21
PRIMER_RIGHT_17=1473,21
PRIMER_LEFT_17_TM=59.761
PRIMER_RIGHT_17_TM=59.583
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=709
PRIMER_PAIR_18_PENALTY=2.656503
PRIMER_LEFT_18_PENALTY=1.239519
PRIMER_RIGHT_18_PENALTY=1.416984
PRIMER_LEFT_18_SEQUENCE=TCTTTGACAGAGTGAGCACGA
PRIMER_RIGHT_18_SEQUENCE=ACGGATGAGGAAAACAGTTGA
PRIMER_LEFT_18=652,21
PRIMER_RIGHT_18=1473,21
PRIMER_LEFT_18_TM=59.760
PRIMER_RIGHT_18_TM=59.583
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=822
PRIMER_PAIR_19_PENALTY=2.666196
PRIMER_LEFT_19_PENALTY=1.249212
PRIMER_RIGHT_19_PENALTY=1.416984
PRIMER_LEFT_19_SEQUENCE=CATGGCGACACTATCTCGTT
PRIMER_RIGHT_19_SEQUENCE=ACGGATGAGGAAAACAGTTGA
PRIMER_LEFT_19=763,20
PRIMER_RIGHT_19=1473,21
PRIMER_LEFT_19_TM=58.751
PRIMER_RIGHT_19_TM=59.583
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=711
PRIMER_PAIR_20_PENALTY=2.677805
PRIMER_LEFT_20_PENALTY=0.816764
PRIMER_RIGHT_20_PENALTY=1.861041
PRIMER_LEFT_20_SEQUENCE=CGGTGAAGCTAACGGAGTTG
PRIMER_RIGHT_20_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_20=233,20
PRIMER_RIGHT_20=1175,21
PRIMER_LEFT_20_TM=60.817
PRIMER_RIGHT_20_TM=59.139
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=943
PRIMER_PAIR_21_PENALTY=2.707732
PRIMER_LEFT_21_PENALTY=0.405717
PRIMER_RIGHT_21_PENALTY=2.302015
PRIMER_LEFT_21_SEQUENCE=GAGCACGAATCTTGGCAACT
PRIMER_RIGHT_21_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_21=665,20
PRIMER_RIGHT_21=1174,20
PRIMER_LEFT_21_TM=60.406
PRIMER_RIGHT_21_TM=57.698
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=510
PRIMER_PAIR_22_PENALTY=2.838192
PRIMER_LEFT_22_PENALTY=0.405717
PRIMER_RIGHT_22_PENALTY=2.432475
PRIMER_LEFT_22_SEQUENCE=GAGCACGAATCTTGGCAACT
PRIMER_RIGHT_22_SEQUENCE=AAACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_22=665,20
PRIMER_RIGHT_22=1176,22
PRIMER_LEFT_22_TM=60.406
PRIMER_RIGHT_22_TM=60.432
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=512
PRIMER_PAIR_23_PENALTY=2.977467
PRIMER_LEFT_23_PENALTY=1.672465
PRIMER_RIGHT_23_PENALTY=1.305002
PRIMER_LEFT_23_SEQUENCE=TCATGGCGACACTATCTCGTT
PRIMER_RIGHT_23_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_23=762,21
PRIMER_RIGHT_23=1472,20
PRIMER_LEFT_23_TM=60.672
PRIMER_RIGHT_23_TM=58.695
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=711
PRIMER_PAIR_24_PENALTY=2.996324
PRIMER_LEFT_24_PENALTY=1.135283
PRIMER_RIGHT_24_PENALTY=1.861041
PRIMER_LEFT_24_SEQUENCE=AACGGTGAAGCTAACGGAGT
PRIMER_RIGHT_24_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_24=231,20
PRIMER_RIGHT_24=1175,21
PRIMER_LEFT_24_TM=58.865
PRIMER_RIGHT_24_TM=59.139
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=945
PRIMER_PAIR_25_PENALTY=3.054612
PRIMER_LEFT_25_PENALTY=1.672465
PRIMER_RIGHT_25_PENALTY=1.382147
PRIMER_LEFT_25_SEQUENCE=TCATGGCGACACTATCTCGTT
PRIMER_RIGHT_25_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_25=762,21
PRIMER_RIGHT_25=1484,21
PRIMER_LEFT_25_TM=60.672
PRIMER_RIGHT_25_TM=59.618
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=723
PRIMER_PAIR_26_PENALTY=3.081343
PRIMER_LEFT_26_PENALTY=1.776341
PRIMER_RIGHT_26_PENALTY=1.305002
PRIMER_LEFT_26_SEQUENCE=CTTTGACAGAGTGAGCACGAA
PRIMER_RIGHT_26_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_26=653,21
PRIMER_RIGHT_26=1472,20
PRIMER_LEFT_26_TM=59.224
PRIMER_RIGHT_26_TM=58.695
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=820
PRIMER_PAIR_27_PENALTY=3.089449
PRIMER_LEFT_27_PENALTY=1.672465
PRIMER_RIGHT_27_PENALTY=1.416984
PRIMER_LEFT_27_SEQUENCE=TCATGGCGACACTATCTCGTT
PRIMER_RIGHT_27_SEQUENCE=ACGGATGAGGAAAACAGTTGA
PRIMER_LEFT_27=762,21
PRIMER_RIGHT_27=1473,21
PRIMER_LEFT_27_TM=60.672
PRIMER_RIGHT_27_TM=59.583
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=712
PRIMER_PAIR_28_PENALTY=3.100559
PRIMER_LEFT_28_PENALTY=1.239519
PRIMER_RIGHT_28_PENALTY=1.861041
PRIMER_LEFT_28_SEQUENCE=TCTTTGACAGAGTGAGCACGA
PRIMER_RIGHT_28_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_28=652,21
PRIMER_RIGHT_28=1175,21
PRIMER_LEFT_28_TM=59.760
PRIMER_RIGHT_28_TM=59.139
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=524
PRIMER_PAIR_29_PENALTY=3.118780
PRIMER_LEFT_29_PENALTY=0.816764
PRIMER_RIGHT_29_PENALTY=2.302015
PRIMER_LEFT_29_SEQUENCE=CGGTGAAGCTAACGGAGTTG
PRIMER_RIGHT_29_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_29=233,20
PRIMER_RIGHT_29=1174,20
PRIMER_LEFT_29_TM=60.817
PRIMER_RIGHT_29_TM=57.698
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=942
PRIMER_PAIR_30_PENALTY=3.158488
PRIMER_LEFT_30_PENALTY=1.776341
PRIMER_RIGHT_30_PENALTY=1.382147
PRIMER_LEFT_30_SEQUENCE=CTTTGACAGAGTGAGCACGAA
PRIMER_RIGHT_30_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_30=653,21
PRIMER_RIGHT_30=1484,21
PRIMER_LEFT_30_TM=59.224
PRIMER_RIGHT_30_TM=59.618
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.4000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=832
PRIMER_PAIR_31_PENALTY=3.193325
PRIMER_LEFT_31_PENALTY=1.776341
PRIMER_RIGHT_31_PENALTY=1.416984
PRIMER_LEFT_31_SEQUENCE=CTTTGACAGAGTGAGCACGAA
PRIMER_RIGHT_31_SEQUENCE=ACGGATGAGGAAAACAGTTGA
PRIMER_LEFT_31=653,21
PRIMER_RIGHT_31=1473,21
PRIMER_LEFT_31_TM=59.224
PRIMER_RIGHT_31_TM=59.583
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=821
PRIMER_PAIR_32_PENALTY=3.193327
PRIMER_LEFT_32_PENALTY=1.888325
PRIMER_RIGHT_32_PENALTY=1.305002
PRIMER_LEFT_32_SEQUENCE=TTTGACAGAGTGAGCACGAA
PRIMER_RIGHT_32_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_32=654,20
PRIMER_RIGHT_32=1472,20
PRIMER_LEFT_32_TM=58.112
PRIMER_RIGHT_32_TM=58.695
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=819
PRIMER_PAIR_33_PENALTY=3.249239
PRIMER_LEFT_33_PENALTY=0.816764
PRIMER_RIGHT_33_PENALTY=2.432475
PRIMER_LEFT_33_SEQUENCE=CGGTGAAGCTAACGGAGTTG
PRIMER_RIGHT_33_SEQUENCE=AAACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_33=233,20
PRIMER_RIGHT_33=1176,22
PRIMER_LEFT_33_TM=60.817
PRIMER_RIGHT_33_TM=60.432
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=40.909
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=944
PRIMER_PAIR_34_PENALTY=3.270472
PRIMER_LEFT_34_PENALTY=1.888325
PRIMER_RIGHT_34_PENALTY=1.382147
PRIMER_LEFT_34_SEQUENCE=TTTGACAGAGTGAGCACGAA
PRIMER_RIGHT_34_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_34=654,20
PRIMER_RIGHT_34=1484,21
PRIMER_LEFT_34_TM=58.112
PRIMER_RIGHT_34_TM=59.618
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.4000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=831
PRIMER_PAIR_35_PENALTY=3.305309
PRIMER_LEFT_35_PENALTY=1.888325
PRIMER_RIGHT_35_PENALTY=1.416984
PRIMER_LEFT_35_SEQUENCE=TTTGACAGAGTGAGCACGAA
PRIMER_RIGHT_35_SEQUENCE=ACGGATGAGGAAAACAGTTGA
PRIMER_LEFT_35=654,20
PRIMER_RIGHT_35=1473,21
PRIMER_LEFT_35_TM=58.112
PRIMER_RIGHT_35_TM=59.583
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.4000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=820
PRIMER_PAIR_36_PENALTY=3.305535
PRIMER_LEFT_36_PENALTY=1.444495
PRIMER_RIGHT_36_PENALTY=1.861041
PRIMER_LEFT_36_SEQUENCE=TGTGTATTGCCGCCAGTTTT
PRIMER_RIGHT_36_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_36=465,20
PRIMER_RIGHT_36=1175,21
PRIMER_LEFT_36_TM=61.444
PRIMER_RIGHT_36_TM=59.139
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=711
PRIMER_PAIR_37_PENALTY=3.305535
PRIMER_LEFT_37_PENALTY=1.444495
PRIMER_RIGHT_37_PENALTY=1.861041
PRIMER_LEFT_37_SEQUENCE=TTGTGTATTGCCGCCAGTTT
PRIMER_RIGHT_37_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_37=464,20
PRIMER_RIGHT_37=1175,21
PRIMER_LEFT_37_TM=61.444
PRIMER_RIGHT_37_TM=59.139
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=712
PRIMER_PAIR_38_PENALTY=3.332717
PRIMER_LEFT_38_PENALTY=2.027715
PRIMER_RIGHT_38_PENALTY=1.305002
PRIMER_LEFT_38_SEQUENCE=ATGGCGACACTATCTCGTTTTT
PRIMER_RIGHT_38_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_38=764,22
PRIMER_RIGHT_38=1472,20
PRIMER_LEFT_38_TM=60.028
PRIMER_RIGHT_38_TM=58.695
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=709
PRIMER_PAIR_39_PENALTY=3.409862
PRIMER_LEFT_39_PENALTY=2.027715
PRIMER_RIGHT_39_PENALTY=1.382147
PRIMER_LEFT_39_SEQUENCE=ATGGCGACACTATCTCGTTTTT
PRIMER_RIGHT_39_SEQUENCE=CGTTGTTTAAGACGGATGAGG
PRIMER_LEFT_39=764,22
PRIMER_RIGHT_39=1484,21
PRIMER_LEFT_39_TM=60.028
PRIMER_RIGHT_39_TM=59.618
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=721
PRIMER_PAIR_40_PENALTY=3.437299
PRIMER_LEFT_40_PENALTY=1.135283
PRIMER_RIGHT_40_PENALTY=2.302015
PRIMER_LEFT_40_SEQUENCE=AACGGTGAAGCTAACGGAGT
PRIMER_RIGHT_40_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_40=231,20
PRIMER_RIGHT_40=1174,20
PRIMER_LEFT_40_TM=58.865
PRIMER_RIGHT_40_TM=57.698
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=944
PRIMER_PAIR_41_PENALTY=3.444699
PRIMER_LEFT_41_PENALTY=2.027715
PRIMER_RIGHT_41_PENALTY=1.416984
PRIMER_LEFT_41_SEQUENCE=ATGGCGACACTATCTCGTTTTT
PRIMER_RIGHT_41_SEQUENCE=ACGGATGAGGAAAACAGTTGA
PRIMER_LEFT_41=764,22
PRIMER_RIGHT_41=1473,21
PRIMER_LEFT_41_TM=60.028
PRIMER_RIGHT_41_TM=59.583
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=710
PRIMER_PAIR_42_PENALTY=3.481762
PRIMER_LEFT_42_PENALTY=1.620721
PRIMER_RIGHT_42_PENALTY=1.861041
PRIMER_LEFT_42_SEQUENCE=TGGCGACACTATCTCGTTTT
PRIMER_RIGHT_42_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_42=765,20
PRIMER_RIGHT_42=1175,21
PRIMER_LEFT_42_TM=58.379
PRIMER_RIGHT_42_TM=59.139
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=411
PRIMER_PAIR_43_PENALTY=3.513087
PRIMER_LEFT_43_PENALTY=0.405717
PRIMER_RIGHT_43_PENALTY=3.107370
PRIMER_LEFT_43_SEQUENCE=GAGCACGAATCTTGGCAACT
PRIMER_RIGHT_43_SEQUENCE=TCGTTGTTTAAGACGGATGAG
PRIMER_LEFT_43=665,20
PRIMER_RIGHT_43=1485,21
PRIMER_LEFT_43_TM=60.406
PRIMER_RIGHT_43_TM=57.893
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=821
PRIMER_PAIR_44_PENALTY=3.536265
PRIMER_LEFT_44_PENALTY=1.675224
PRIMER_RIGHT_44_PENALTY=1.861041
PRIMER_LEFT_44_SEQUENCE=GAACGGTGAAGCTAACGGAGT
PRIMER_RIGHT_44_SEQUENCE=AACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_44=230,21
PRIMER_RIGHT_44=1175,21
PRIMER_LEFT_44_TM=60.675
PRIMER_RIGHT_44_TM=59.139
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=946
PRIMER_PAIR_45_PENALTY=3.541534
PRIMER_LEFT_45_PENALTY=1.239519
PRIMER_RIGHT_45_PENALTY=2.302015
PRIMER_LEFT_45_SEQUENCE=TCTTTGACAGAGTGAGCACGA
PRIMER_RIGHT_45_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_45=652,21
PRIMER_RIGHT_45=1174,20
PRIMER_LEFT_45_TM=59.760
PRIMER_RIGHT_45_TM=57.698
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.4000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=523
PRIMER_PAIR_46_PENALTY=3.551227
PRIMER_LEFT_46_PENALTY=1.249212
PRIMER_RIGHT_46_PENALTY=2.302015
PRIMER_LEFT_46_SEQUENCE=CATGGCGACACTATCTCGTT
PRIMER_RIGHT_46_SEQUENCE=ACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_46=763,20
PRIMER_RIGHT_46=1174,20
PRIMER_LEFT_46_TM=58.751
PRIMER_RIGHT_46_TM=57.698
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=412
PRIMER_PAIR_47_PENALTY=3.567759
PRIMER_LEFT_47_PENALTY=1.135283
PRIMER_RIGHT_47_PENALTY=2.432475
PRIMER_LEFT_47_SEQUENCE=AACGGTGAAGCTAACGGAGT
PRIMER_RIGHT_47_SEQUENCE=AAACGAGAAATGAGCTGTGCTT
PRIMER_LEFT_47=231,20
PRIMER_RIGHT_47=1176,22
PRIMER_LEFT_47_TM=58.865
PRIMER_RIGHT_47_TM=60.432
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=946
PRIMER_PAIR_48_PENALTY=3.576200
PRIMER_LEFT_48_PENALTY=0.405717
PRIMER_RIGHT_48_PENALTY=3.170483
PRIMER_LEFT_48_SEQUENCE=GAGCACGAATCTTGGCAACT
PRIMER_RIGHT_48_SEQUENCE=AAGACGGATGAGGAAAACAG
PRIMER_LEFT_48=665,20
PRIMER_RIGHT_48=1476,20
PRIMER_LEFT_48_TM=60.406
PRIMER_RIGHT_48_TM=56.830
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=812
PRIMER_PAIR_49_PENALTY=3.631231
PRIMER_LEFT_49_PENALTY=2.326229
PRIMER_RIGHT_49_PENALTY=1.305002
PRIMER_LEFT_49_SEQUENCE=CTTTGACAGAGTGAGCACGA
PRIMER_RIGHT_49_SEQUENCE=CGGATGAGGAAAACAGTTGA
PRIMER_LEFT_49=653,20
PRIMER_RIGHT_49=1472,20
PRIMER_LEFT_49_TM=57.674
PRIMER_RIGHT_49_TM=58.695
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.4000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=820
=
SEQUENCE_ID=MAGIC.446_4_2590253_2590563_tsu-0_zu-0_4826_189637_TRUE
SEQUENCE_TARGET=950,410
SEQUENCE_TEMPLATE=CGAAACAAATCGAAGAAATACAATAAGAANTCAGANATNTACCTTGATCACGGTNGAGANATTTGAGAGATTGATTTNAGAGTTNGAGNAANNTGGAATCTGAATTGTGAAGAAGAAGAGCTCTTTGGGTATTTGATTTATAGAAGCAANAAAGACGAGNACGNCTCGGTCGGTCACGAGAAAGCTAANGCGTGGAAGCAATAGCCAATTATANATTGACACGTATGTGTAGGGACGTGTACGCGTTGTTCACACGCANAGTTGNTTTTGNCTAATTGCCTTATTTTTAGNTTNAGGAAAAGTANTTTGTGCATTGAGTTGTNAAANATTNGATGTAAANATAGTTTGCTGCGAGACATATACTGAANGTTGAATTACNATTNNNNNAGNTCATTAGATNATCTNGATANGTATGCCAAGAAGTTCTCTGNGTNGAAANTTGAATTAAGCANAACTGAGAGACATNTACTGAAAGAGATGGGTTTTGTTTGTCATGTTGNANANTAGAAACACCTCCNGAATTGAGGCNNNAAGCTTGGAATTTNGCTAATGATAGTCTNCGTANANCCNTTTGTGTAAGGTTCACAAGTGAGGTTGTGGNTTGTGGGGTCGTGTATGCTGCTGCNCGTAGGTTTCAAGTACCTCTCCCNGAGAATNCACCCTGNTGNAANGCATTTGATGCAGATAAATCTANNNNNNNNNNNNNNNGNANNNTTNTNGNTCATCTATACACTCTTCCAAAGGCTTANTATATCTCNTNTGTTTGNAAGGATTCAGGTGGACANTCAGATAAATCAAGACATCACTCTTTTNCAAANATCCTTTTCANNNNNNNNNNNNNNNNNNNTCCTCNGCAAATNATTTTGGGATTACTAAGANAANTGATCATAAATACAATCTTCTATCTCNTCACACAACANATTTTGANTCGGCACAAANTNAAAAAANAAAAANACATNNACACACTTTTTGGTCTTGGTGGTNTTTAAGTTTCAAGCAGNGTCTACTCCTCCTTGTTGNTGTGCAAGATATCTCTCTCGGCGTTGCTTCTGGTCATTAGTACAGAAGATAAACACAGTNANCATCANTATCACANTTTCTTTGTTGTCTTNTAAAACAAAACANAAAAANAAGACNNGAGTAAGGCATGAAAANAATGGCATNCGTCTCTCACCCATTCATCGATCACAAGCCCTTCTCCAATTGGNTTTGGACCAACAATACCNTCTGATGCTTTNTTCCGTGCTTCATCTGCAGCATCCGCTTCAGCCAATTGACTCTTCAATTTCTCCATCATCTGTAAAGAGAGANGTAACAAAGNTTAAAGAATCAGTGATATCTANNATTNCTTAAAGCNNNAAATGATCCCAATCAAGTTCACAAATGATGGCGANAAGAANCAGAAAAAGAAGAAGAAGCNGACNGACTCACGGNGTTAGGGTTTTCGGGATCCAAGAATACCACACGCATTACTTGCTCCTCAGAGACATNATCTTTTTGCTCCTCAAACTGCAAAACACAAGAGCATCCACACTTCTTAGAATCCAANAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAGTTACTANAAGTTTCACATACCAGCTCAGGCATATAGTCTGTTGGNACTTTCATCTTCANGCTCATGATTTTAAACTTAACCCCGCTCTTTTGTTCCATNGGCTTTTCATTATTCTCAGGAGGCTCAACAAACTTGAAAACTAAAGAATTCAACAAGACATAGAAACNAACAGTCACCACANAATCNTNGAGGCCAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAGAAGCAAATACCAGTTGCNATAATTTCTTCCCCNGGAGCAAGAATAGCACCAGCNGGNCGCATGAAGCANCTCTTTGGTACTGTTGTTTGAAACTGNNNAAACCAAGATAANCAGCAGANATAAACACCAATNACAACAACAACAACCATAATCAAAACGTTTNTGAATCCATTNCAAACCTTAAACGCTACATGTGACTTGCTCGTATTCTTAATCTTAATNGCACTCCTGACTTGCTTCCCAGGCTCATCTACANACAAAGTTCATAAACTAAATNACTAATCTTNGATCATCNATAAAGAGTGAATCTTGAAATTGACAAGACTCCACATTTTATTCCTAAATTTGCTCAATGGCNNNNNNNNNTTTATNGTAACGTATCAATAAGTCAAATTACAAGTCAACATCATCATNNNCTTCAATATATATCCAATTTCATGATTCNCAAAGATAAAGCTAAACCAG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.043189
PRIMER_LEFT_0_PENALTY=2.931824
PRIMER_RIGHT_0_PENALTY=0.111365
PRIMER_LEFT_0_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_0_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_0=601,20
PRIMER_RIGHT_0=1391,20
PRIMER_LEFT_0_TM=62.932
PRIMER_RIGHT_0_TM=60.111
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=791
PRIMER_PAIR_1_PENALTY=3.346980
PRIMER_LEFT_1_PENALTY=2.931824
PRIMER_RIGHT_1_PENALTY=0.415156
PRIMER_LEFT_1_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_1_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_1=601,20
PRIMER_RIGHT_1=1513,20
PRIMER_LEFT_1_TM=62.932
PRIMER_RIGHT_1_TM=59.585
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=913
PRIMER_PAIR_2_PENALTY=3.346980
PRIMER_LEFT_2_PENALTY=2.931824
PRIMER_RIGHT_2_PENALTY=0.415156
PRIMER_LEFT_2_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_2_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_2=601,20
PRIMER_RIGHT_2=1514,20
PRIMER_LEFT_2_TM=62.932
PRIMER_RIGHT_2_TM=59.585
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=914
PRIMER_PAIR_3_PENALTY=3.507807
PRIMER_LEFT_3_PENALTY=3.396441
PRIMER_RIGHT_3_PENALTY=0.111365
PRIMER_LEFT_3_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_3_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_3=626,23
PRIMER_RIGHT_3=1391,20
PRIMER_LEFT_3_TM=60.396
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=766
PRIMER_PAIR_4_PENALTY=3.534415
PRIMER_LEFT_4_PENALTY=3.423050
PRIMER_RIGHT_4_PENALTY=0.111365
PRIMER_LEFT_4_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_4_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_4=603,20
PRIMER_RIGHT_4=1391,20
PRIMER_LEFT_4_TM=63.423
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=789
PRIMER_PAIR_5_PENALTY=3.751869
PRIMER_LEFT_5_PENALTY=3.640503
PRIMER_RIGHT_5_PENALTY=0.111365
PRIMER_LEFT_5_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_5_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_5=479,20
PRIMER_RIGHT_5=1391,20
PRIMER_LEFT_5_TM=56.359
PRIMER_RIGHT_5_TM=60.111
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=913
PRIMER_PAIR_6_PENALTY=3.811598
PRIMER_LEFT_6_PENALTY=3.396441
PRIMER_RIGHT_6_PENALTY=0.415156
PRIMER_LEFT_6_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_6_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_6=626,23
PRIMER_RIGHT_6=1513,20
PRIMER_LEFT_6_TM=60.396
PRIMER_RIGHT_6_TM=59.585
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=888
PRIMER_PAIR_7_PENALTY=3.811598
PRIMER_LEFT_7_PENALTY=3.396441
PRIMER_RIGHT_7_PENALTY=0.415156
PRIMER_LEFT_7_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_7_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_7=626,23
PRIMER_RIGHT_7=1514,20
PRIMER_LEFT_7_TM=60.396
PRIMER_RIGHT_7_TM=59.585
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=889
PRIMER_PAIR_8_PENALTY=3.881760
PRIMER_LEFT_8_PENALTY=2.931824
PRIMER_RIGHT_8_PENALTY=0.949937
PRIMER_LEFT_8_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_8_SEQUENCE=GTTTTGCAGTTTGAGGAGCA
PRIMER_LEFT_8=601,20
PRIMER_RIGHT_8=1517,20
PRIMER_LEFT_8_TM=62.932
PRIMER_RIGHT_8_TM=59.050
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=917
PRIMER_PAIR_9_PENALTY=3.929988
PRIMER_LEFT_9_PENALTY=2.931824
PRIMER_RIGHT_9_PENALTY=0.998165
PRIMER_LEFT_9_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_9_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_9=601,20
PRIMER_RIGHT_9=1469,20
PRIMER_LEFT_9_TM=62.932
PRIMER_RIGHT_9_TM=59.002
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=2.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=869
PRIMER_PAIR_10_PENALTY=3.973793
PRIMER_LEFT_10_PENALTY=2.931824
PRIMER_RIGHT_10_PENALTY=1.041969
PRIMER_LEFT_10_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_10_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_10=601,20
PRIMER_RIGHT_10=1453,20
PRIMER_LEFT_10_TM=62.932
PRIMER_RIGHT_10_TM=61.042
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=5.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=853
PRIMER_PAIR_11_PENALTY=3.979923
PRIMER_LEFT_11_PENALTY=2.931824
PRIMER_RIGHT_11_PENALTY=1.048100
PRIMER_LEFT_11_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_11_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_11=601,20
PRIMER_RIGHT_11=1480,20
PRIMER_LEFT_11_TM=62.932
PRIMER_RIGHT_11_TM=58.952
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=880
PRIMER_PAIR_12_PENALTY=4.127860
PRIMER_LEFT_12_PENALTY=2.931824
PRIMER_RIGHT_12_PENALTY=1.196037
PRIMER_LEFT_12_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_12_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_12=601,20
PRIMER_RIGHT_12=1478,20
PRIMER_LEFT_12_TM=62.932
PRIMER_RIGHT_12_TM=58.804
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=878
PRIMER_PAIR_13_PENALTY=4.183085
PRIMER_LEFT_13_PENALTY=2.931824
PRIMER_RIGHT_13_PENALTY=1.251261
PRIMER_LEFT_13_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_13_SEQUENCE=TGTGGATGCTCTTGTGTTTTG
PRIMER_LEFT_13=601,20
PRIMER_RIGHT_13=1532,21
PRIMER_LEFT_13_TM=62.932
PRIMER_RIGHT_13_TM=59.749
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=932
PRIMER_PAIR_14_PENALTY=4.215867
PRIMER_LEFT_14_PENALTY=3.800711
PRIMER_RIGHT_14_PENALTY=0.415156
PRIMER_LEFT_14_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_14_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_14=574,23
PRIMER_RIGHT_14=1513,20
PRIMER_LEFT_14_TM=59.199
PRIMER_RIGHT_14_TM=59.585
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=940
PRIMER_PAIR_15_PENALTY=4.215867
PRIMER_LEFT_15_PENALTY=3.800711
PRIMER_RIGHT_15_PENALTY=0.415156
PRIMER_LEFT_15_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_15_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_15=574,23
PRIMER_RIGHT_15=1514,20
PRIMER_LEFT_15_TM=59.199
PRIMER_RIGHT_15_TM=59.585
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=941
PRIMER_PAIR_16_PENALTY=4.308600
PRIMER_LEFT_16_PENALTY=2.931824
PRIMER_RIGHT_16_PENALTY=1.376776
PRIMER_LEFT_16_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_16_SEQUENCE=GCAGTTTGAGGAGCAAAAAGA
PRIMER_LEFT_16=601,20
PRIMER_RIGHT_16=1512,21
PRIMER_LEFT_16_TM=62.932
PRIMER_RIGHT_16_TM=59.623
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=912
PRIMER_PAIR_17_PENALTY=4.311754
PRIMER_LEFT_17_PENALTY=2.931824
PRIMER_RIGHT_17_PENALTY=1.379931
PRIMER_LEFT_17_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_17_SEQUENCE=AATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_17=601,20
PRIMER_RIGHT_17=1470,21
PRIMER_LEFT_17_TM=62.932
PRIMER_RIGHT_17_TM=60.380
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=2.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=870
PRIMER_PAIR_18_PENALTY=4.346378
PRIMER_LEFT_18_PENALTY=3.396441
PRIMER_RIGHT_18_PENALTY=0.949937
PRIMER_LEFT_18_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_18_SEQUENCE=GTTTTGCAGTTTGAGGAGCA
PRIMER_LEFT_18=626,23
PRIMER_RIGHT_18=1517,20
PRIMER_LEFT_18_TM=60.396
PRIMER_RIGHT_18_TM=59.050
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=892
PRIMER_PAIR_19_PENALTY=4.394606
PRIMER_LEFT_19_PENALTY=3.396441
PRIMER_RIGHT_19_PENALTY=0.998165
PRIMER_LEFT_19_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_19_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_19=626,23
PRIMER_RIGHT_19=1469,20
PRIMER_LEFT_19_TM=60.396
PRIMER_RIGHT_19_TM=59.002
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=844
PRIMER_PAIR_20_PENALTY=4.415611
PRIMER_LEFT_20_PENALTY=4.000455
PRIMER_RIGHT_20_PENALTY=0.415156
PRIMER_LEFT_20_SEQUENCE=GTGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_20_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_20=573,24
PRIMER_RIGHT_20=1513,20
PRIMER_LEFT_20_TM=60.000
PRIMER_RIGHT_20_TM=59.585
PRIMER_LEFT_20_GC_PERCENT=45.833
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=941
PRIMER_PAIR_21_PENALTY=4.415611
PRIMER_LEFT_21_PENALTY=4.000455
PRIMER_RIGHT_21_PENALTY=0.415156
PRIMER_LEFT_21_SEQUENCE=GTGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_21_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_21=573,24
PRIMER_RIGHT_21=1514,20
PRIMER_LEFT_21_TM=60.000
PRIMER_RIGHT_21_TM=59.585
PRIMER_LEFT_21_GC_PERCENT=45.833
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=942
PRIMER_PAIR_22_PENALTY=4.421215
PRIMER_LEFT_22_PENALTY=3.423050
PRIMER_RIGHT_22_PENALTY=0.998165
PRIMER_LEFT_22_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_22_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_22=603,20
PRIMER_RIGHT_22=1469,20
PRIMER_LEFT_22_TM=63.423
PRIMER_RIGHT_22_TM=59.002
PRIMER_LEFT_22_GC_PERCENT=60.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=867
PRIMER_PAIR_23_PENALTY=4.429486
PRIMER_LEFT_23_PENALTY=2.931824
PRIMER_RIGHT_23_PENALTY=1.497663
PRIMER_LEFT_23_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_23_SEQUENCE=AATGCGTGTGGTATTCTTGG
PRIMER_LEFT_23=601,20
PRIMER_RIGHT_23=1470,20
PRIMER_LEFT_23_TM=62.932
PRIMER_RIGHT_23_TM=58.502
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=870
PRIMER_PAIR_24_PENALTY=4.438410
PRIMER_LEFT_24_PENALTY=3.396441
PRIMER_RIGHT_24_PENALTY=1.041969
PRIMER_LEFT_24_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_24_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_24=626,23
PRIMER_RIGHT_24=1453,20
PRIMER_LEFT_24_TM=60.396
PRIMER_RIGHT_24_TM=61.042
PRIMER_LEFT_24_GC_PERCENT=52.174
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=5.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=828
PRIMER_PAIR_25_PENALTY=4.443762
PRIMER_LEFT_25_PENALTY=2.931824
PRIMER_RIGHT_25_PENALTY=1.511938
PRIMER_LEFT_25_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_25_SEQUENCE=TTGGATCCCGAAAACCCTAA
PRIMER_LEFT_25=601,20
PRIMER_RIGHT_25=1454,20
PRIMER_LEFT_25_TM=62.932
PRIMER_RIGHT_25_TM=61.512
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=7.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=854
PRIMER_PAIR_26_PENALTY=4.444541
PRIMER_LEFT_26_PENALTY=3.396441
PRIMER_RIGHT_26_PENALTY=1.048100
PRIMER_LEFT_26_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_26_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_26=626,23
PRIMER_RIGHT_26=1480,20
PRIMER_LEFT_26_TM=60.396
PRIMER_RIGHT_26_TM=58.952
PRIMER_LEFT_26_GC_PERCENT=52.174
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=855
PRIMER_PAIR_27_PENALTY=4.465019
PRIMER_LEFT_27_PENALTY=3.423050
PRIMER_RIGHT_27_PENALTY=1.041969
PRIMER_LEFT_27_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_27_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_27=603,20
PRIMER_RIGHT_27=1453,20
PRIMER_LEFT_27_TM=63.423
PRIMER_RIGHT_27_TM=61.042
PRIMER_LEFT_27_GC_PERCENT=60.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=5.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=851
PRIMER_PAIR_28_PENALTY=4.471150
PRIMER_LEFT_28_PENALTY=3.423050
PRIMER_RIGHT_28_PENALTY=1.048100
PRIMER_LEFT_28_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_28_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_28=603,20
PRIMER_RIGHT_28=1480,20
PRIMER_LEFT_28_TM=63.423
PRIMER_RIGHT_28_TM=58.952
PRIMER_LEFT_28_GC_PERCENT=60.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=878
PRIMER_PAIR_29_PENALTY=4.471867
PRIMER_LEFT_29_PENALTY=2.931824
PRIMER_RIGHT_29_PENALTY=1.540043
PRIMER_LEFT_29_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_29_SEQUENCE=TGCAGTTTGAGGAGCAAAAAG
PRIMER_LEFT_29=601,20
PRIMER_RIGHT_29=1513,21
PRIMER_LEFT_29_TM=62.932
PRIMER_RIGHT_29_TM=60.540
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=913
PRIMER_PAIR_30_PENALTY=4.562495
PRIMER_LEFT_30_PENALTY=2.931824
PRIMER_RIGHT_30_PENALTY=1.630671
PRIMER_LEFT_30_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_30_SEQUENCE=GGAGCAAGTAATGCGTGTGG
PRIMER_LEFT_30=601,20
PRIMER_RIGHT_30=1479,20
PRIMER_LEFT_30_TM=62.932
PRIMER_RIGHT_30_TM=61.631
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=879
PRIMER_PAIR_31_PENALTY=4.592478
PRIMER_LEFT_31_PENALTY=3.396441
PRIMER_RIGHT_31_PENALTY=1.196037
PRIMER_LEFT_31_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_31_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_31=626,23
PRIMER_RIGHT_31=1478,20
PRIMER_LEFT_31_TM=60.396
PRIMER_RIGHT_31_TM=58.804
PRIMER_LEFT_31_GC_PERCENT=52.174
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=853
PRIMER_PAIR_32_PENALTY=4.619087
PRIMER_LEFT_32_PENALTY=3.423050
PRIMER_RIGHT_32_PENALTY=1.196037
PRIMER_LEFT_32_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_32_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_32=603,20
PRIMER_RIGHT_32=1478,20
PRIMER_LEFT_32_TM=63.423
PRIMER_RIGHT_32_TM=58.804
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=876
PRIMER_PAIR_33_PENALTY=4.638668
PRIMER_LEFT_33_PENALTY=3.640503
PRIMER_RIGHT_33_PENALTY=0.998165
PRIMER_LEFT_33_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_33_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_33=479,20
PRIMER_RIGHT_33=1469,20
PRIMER_LEFT_33_TM=56.359
PRIMER_RIGHT_33_TM=59.002
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=991
PRIMER_PAIR_34_PENALTY=4.647703
PRIMER_LEFT_34_PENALTY=3.396441
PRIMER_RIGHT_34_PENALTY=1.251261
PRIMER_LEFT_34_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_34_SEQUENCE=TGTGGATGCTCTTGTGTTTTG
PRIMER_LEFT_34=626,23
PRIMER_RIGHT_34=1532,21
PRIMER_LEFT_34_TM=60.396
PRIMER_RIGHT_34_TM=59.749
PRIMER_LEFT_34_GC_PERCENT=52.174
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=907
PRIMER_PAIR_35_PENALTY=4.674311
PRIMER_LEFT_35_PENALTY=3.423050
PRIMER_RIGHT_35_PENALTY=1.251261
PRIMER_LEFT_35_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_35_SEQUENCE=TGTGGATGCTCTTGTGTTTTG
PRIMER_LEFT_35=603,20
PRIMER_RIGHT_35=1532,21
PRIMER_LEFT_35_TM=63.423
PRIMER_RIGHT_35_TM=59.749
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=930
PRIMER_PAIR_36_PENALTY=4.682473
PRIMER_LEFT_36_PENALTY=3.640503
PRIMER_RIGHT_36_PENALTY=1.041969
PRIMER_LEFT_36_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_36_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_36=479,20
PRIMER_RIGHT_36=1453,20
PRIMER_LEFT_36_TM=56.359
PRIMER_RIGHT_36_TM=61.042
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=5.7000
PRIMER_PAIR_36_COMPL_ANY=7.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=975
PRIMER_PAIR_37_PENALTY=4.727462
PRIMER_LEFT_37_PENALTY=4.312305
PRIMER_RIGHT_37_PENALTY=0.415156
PRIMER_LEFT_37_SEQUENCE=TGTGTAAGGTTCACAAGTGAGGT
PRIMER_RIGHT_37_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_37=572,23
PRIMER_RIGHT_37=1513,20
PRIMER_LEFT_37_TM=58.688
PRIMER_RIGHT_37_TM=59.585
PRIMER_LEFT_37_GC_PERCENT=43.478
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=942
PRIMER_PAIR_38_PENALTY=4.727462
PRIMER_LEFT_38_PENALTY=4.312305
PRIMER_RIGHT_38_PENALTY=0.415156
PRIMER_LEFT_38_SEQUENCE=TGTGTAAGGTTCACAAGTGAGGT
PRIMER_RIGHT_38_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_38=572,23
PRIMER_RIGHT_38=1514,20
PRIMER_LEFT_38_TM=58.688
PRIMER_RIGHT_38_TM=59.585
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=943
PRIMER_PAIR_39_PENALTY=4.750648
PRIMER_LEFT_39_PENALTY=3.800711
PRIMER_RIGHT_39_PENALTY=0.949937
PRIMER_LEFT_39_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_39_SEQUENCE=GTTTTGCAGTTTGAGGAGCA
PRIMER_LEFT_39=574,23
PRIMER_RIGHT_39=1517,20
PRIMER_LEFT_39_TM=59.199
PRIMER_RIGHT_39_TM=59.050
PRIMER_LEFT_39_GC_PERCENT=43.478
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=944
PRIMER_PAIR_40_PENALTY=4.773217
PRIMER_LEFT_40_PENALTY=3.396441
PRIMER_RIGHT_40_PENALTY=1.376776
PRIMER_LEFT_40_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_40_SEQUENCE=GCAGTTTGAGGAGCAAAAAGA
PRIMER_LEFT_40=626,23
PRIMER_RIGHT_40=1512,21
PRIMER_LEFT_40_TM=60.396
PRIMER_RIGHT_40_TM=59.623
PRIMER_LEFT_40_GC_PERCENT=52.174
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=887
PRIMER_PAIR_41_PENALTY=4.774083
PRIMER_LEFT_41_PENALTY=2.931824
PRIMER_RIGHT_41_PENALTY=1.842260
PRIMER_LEFT_41_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_41_SEQUENCE=GAGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_41=601,20
PRIMER_RIGHT_41=1481,21
PRIMER_LEFT_41_TM=62.932
PRIMER_RIGHT_41_TM=60.842
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=881
PRIMER_PAIR_42_PENALTY=4.774083
PRIMER_LEFT_42_PENALTY=2.931824
PRIMER_RIGHT_42_PENALTY=1.842260
PRIMER_LEFT_42_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_42_SEQUENCE=TGAGGAGCAAGTAATGCGTGT
PRIMER_LEFT_42=601,20
PRIMER_RIGHT_42=1482,21
PRIMER_LEFT_42_TM=62.932
PRIMER_RIGHT_42_TM=60.842
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=8.1000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=882
PRIMER_PAIR_43_PENALTY=4.776372
PRIMER_LEFT_43_PENALTY=3.396441
PRIMER_RIGHT_43_PENALTY=1.379931
PRIMER_LEFT_43_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_43_SEQUENCE=AATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_43=626,23
PRIMER_RIGHT_43=1470,21
PRIMER_LEFT_43_TM=60.396
PRIMER_RIGHT_43_TM=60.380
PRIMER_LEFT_43_GC_PERCENT=52.174
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=845
PRIMER_PAIR_44_PENALTY=4.798876
PRIMER_LEFT_44_PENALTY=3.800711
PRIMER_RIGHT_44_PENALTY=0.998165
PRIMER_LEFT_44_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_44_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_44=574,23
PRIMER_RIGHT_44=1469,20
PRIMER_LEFT_44_TM=59.199
PRIMER_RIGHT_44_TM=59.002
PRIMER_LEFT_44_GC_PERCENT=43.478
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=896
PRIMER_PAIR_45_PENALTY=4.802980
PRIMER_LEFT_45_PENALTY=3.423050
PRIMER_RIGHT_45_PENALTY=1.379931
PRIMER_LEFT_45_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_45_SEQUENCE=AATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_45=603,20
PRIMER_RIGHT_45=1470,21
PRIMER_LEFT_45_TM=63.423
PRIMER_RIGHT_45_TM=60.380
PRIMER_LEFT_45_GC_PERCENT=60.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=868
PRIMER_PAIR_46_PENALTY=4.836540
PRIMER_LEFT_46_PENALTY=3.640503
PRIMER_RIGHT_46_PENALTY=1.196037
PRIMER_LEFT_46_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_46_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_46=479,20
PRIMER_RIGHT_46=1478,20
PRIMER_LEFT_46_TM=56.359
PRIMER_RIGHT_46_TM=58.804
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=1000
PRIMER_PAIR_47_PENALTY=4.842680
PRIMER_LEFT_47_PENALTY=3.800711
PRIMER_RIGHT_47_PENALTY=1.041969
PRIMER_LEFT_47_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_47_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_47=574,23
PRIMER_RIGHT_47=1453,20
PRIMER_LEFT_47_TM=59.199
PRIMER_RIGHT_47_TM=61.042
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=5.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=880
PRIMER_PAIR_48_PENALTY=4.848811
PRIMER_LEFT_48_PENALTY=3.800711
PRIMER_RIGHT_48_PENALTY=1.048100
PRIMER_LEFT_48_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_48_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_48=574,23
PRIMER_RIGHT_48=1480,20
PRIMER_LEFT_48_TM=59.199
PRIMER_RIGHT_48_TM=58.952
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=907
PRIMER_PAIR_49_PENALTY=4.894104
PRIMER_LEFT_49_PENALTY=3.396441
PRIMER_RIGHT_49_PENALTY=1.497663
PRIMER_LEFT_49_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_49_SEQUENCE=AATGCGTGTGGTATTCTTGG
PRIMER_LEFT_49=626,23
PRIMER_RIGHT_49=1470,20
PRIMER_LEFT_49_TM=60.396
PRIMER_RIGHT_49_TM=58.502
PRIMER_LEFT_49_GC_PERCENT=52.174
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=845
=
SEQUENCE_ID=MAGIC.175_5_16228539_16228550_rsch-4_can-0_637154_252_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=CTTTGATTCCCCTGAGTAGTTTAAATCAATACATTTTTTAGATTATAATTATCTCGGCGTAACAATGCCAGATTATTTNTTACNCTCATGTGACATCTTTAGAGTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTATTAGAAGAAGACTCTTGTTTGTACCATTGCATNTTTTTCATCANAAGCCTCTGTATGTGTCCGAAGGTTTGATTGTCTTGTTCTTCTCCNTNAAGTGAGANCCACAAATAGACNTAAAAAATATCTTGTGGTCAATNATCTGTNTCTCTANTCTCTGTTTCGTGTTCCANTTCACGANNNNNNNNNNACTTTNCTAAGTTTATNAAGAACATTTCTTTTCAATTTNTCGATTCTTTCTGGCTCATTCTCTCATCTCTNTTTCATATCNAAATTNTTGATTGAGAATTCGACTTGTGGAAATGGCAACAGTTGCATCAAATCTCNCTTGTATCGGAAAAGTAACGNTNTCTAGAGCTANTTCAATGNCAAGTCNTTTTTTNTATNATGCTNGCCAAAGAANTGGNATAAAGAGTGGATTTGGAATGGATTTTGCAGGTAGGANGGAGGTTGCAGATAAATTGCAGAAAGAAAGAGAAAGGAAGAGATCAGAGTCATTATCCTTACAAAGTCATTGAAATCACTCCTCCTCCTAAATCTCTCGGCATTCGTTGCCTTCCTCATGTGAGTCTCAAATCTTTCTCAATGCTCAACTACACTTCATAAGNATTTCANTACAGATTCCAAGTGAGAATGAGAAGCTTTTTTGATTTGCAGAANCTTCANTGTGGAGAGAACGTGATGATCGAAGGGCAAACGTACACAATCTCNGCNGTNACTCANCGGTATCAGCTCCGGAAAGGCAAATACGAGCCGAGTGAGAGGCGGCTCGATGTTCTCTCTGCNGCTAGATACGTTTTAAACCTTTACTTCGACAATTTGNTCCAAAACTCNTGNNAACTTTNATTTAGCNAAAANGTTTGGTCTANNNGATTTTCATTGTATCCATGAATGCTTGTGAATTTGTTAAGCAATNATTTGTANTTCTCTTAATCATTNCACNTTNTATCNCNNATATNNNNCAANNTNNTNGNNTTACNTNTNGNAANNNNNNNNNNNNTANTTTTGNNNATTNNTNNNNNATNNACNANNNATTCNTGNTTTNNCNNAANAANNTNAANACNNANTNNTNNNAANTNNNTTAATGATTTAACNATNCAACAACNCCCCAAAGTTCTTTNTAAAGGAAACNACGACTTCGNTAAACTACCAGGTTAAGGAAATGTATATAGCTAAGAANTCAAGCCAANTGCGTAAATTCTNCATCTTACGCCTCATGGTTTAGCTTCTATAATAAACAAACATTTTGAGTCATCNTCAGGCAATTAGCTTGCAAANAAAATGATATAATACGTCTAATTGCTNCCATAANTTTACAATTTAGTTGACCAACGTNGCTTGATTTAACTAAAACAACTGTCTGTCACCNAACAAACCAAGACGGACGAGTAGATTTGTATGTAANCTCTANACGAATCCGTTTGTGANCTTNATTTGCGTCACCGCCTTGTCACATTCGTGTGGACGCCTCACGACGTGTTTGCATAATGTTANTGAAACAGAAATATTNCACTCTTCCTCCANTTTTTGATTACTTCCATTTCCTCAAAAGTCAAAGCTCTCTTTCTTCTCTCTCGAGATTTGCTAACTTGTCAATGGCGGATGTTGAGAATCTGAACTCTGTTNCGGCGTCGGAAGACACAGAGTATGGATTTAAACGGCCGGAGATGTACAGCACAAACATAGCCAATTCCATAACTTCCTACGCTCGTCATGTTTTTGTTCTTTACAAAACTCCTGAAGCTTGGCTCTCTCACGTCGAAGAAGAAGGTTTACCTCAACGTTTCGCTACGTTGCTTAAAGATCGCAAATCTGATCTTCTTGTTCAGGTTTATACTAAAACGTTTGACATTTTCTTACGTTGTATC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.172529
PRIMER_LEFT_0_PENALTY=0.072951
PRIMER_RIGHT_0_PENALTY=0.099578
PRIMER_LEFT_0_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_0_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_0=816,20
PRIMER_RIGHT_0=1756,20
PRIMER_LEFT_0_TM=60.073
PRIMER_RIGHT_0_TM=59.900
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=941
PRIMER_PAIR_1_PENALTY=0.221461
PRIMER_LEFT_1_PENALTY=0.072951
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_1_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_1=816,20
PRIMER_RIGHT_1=1531,20
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=716
PRIMER_PAIR_2_PENALTY=0.238665
PRIMER_LEFT_2_PENALTY=0.090155
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_2_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_2=670,20
PRIMER_RIGHT_2=1531,20
PRIMER_LEFT_2_TM=59.910
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=862
PRIMER_PAIR_3_PENALTY=0.362015
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_3_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_3=677,20
PRIMER_RIGHT_3=1531,20
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=855
PRIMER_PAIR_4_PENALTY=0.450783
PRIMER_LEFT_4_PENALTY=0.072951
PRIMER_RIGHT_4_PENALTY=0.377832
PRIMER_LEFT_4_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_4_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_4=816,20
PRIMER_RIGHT_4=1532,20
PRIMER_LEFT_4_TM=60.073
PRIMER_RIGHT_4_TM=59.622
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=717
PRIMER_PAIR_5_PENALTY=0.457878
PRIMER_LEFT_5_PENALTY=0.309369
PRIMER_RIGHT_5_PENALTY=0.148509
PRIMER_LEFT_5_SEQUENCE=TTGGAATGGATTTTGCAGGT
PRIMER_RIGHT_5_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_5=563,20
PRIMER_RIGHT_5=1531,20
PRIMER_LEFT_5_TM=60.309
PRIMER_RIGHT_5_TM=60.149
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=969
PRIMER_PAIR_6_PENALTY=0.467987
PRIMER_LEFT_6_PENALTY=0.090155
PRIMER_RIGHT_6_PENALTY=0.377832
PRIMER_LEFT_6_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_6_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_6=670,20
PRIMER_RIGHT_6=1532,20
PRIMER_LEFT_6_TM=59.910
PRIMER_RIGHT_6_TM=59.622
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=11.7000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=863
PRIMER_PAIR_7_PENALTY=0.569563
PRIMER_LEFT_7_PENALTY=0.469985
PRIMER_RIGHT_7_PENALTY=0.099578
PRIMER_LEFT_7_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_7_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_7=821,20
PRIMER_RIGHT_7=1756,20
PRIMER_LEFT_7_TM=60.470
PRIMER_RIGHT_7_TM=59.900
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=936
PRIMER_PAIR_8_PENALTY=0.591338
PRIMER_LEFT_8_PENALTY=0.213506
PRIMER_RIGHT_8_PENALTY=0.377832
PRIMER_LEFT_8_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_8_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_8=677,20
PRIMER_RIGHT_8=1532,20
PRIMER_LEFT_8_TM=60.214
PRIMER_RIGHT_8_TM=59.622
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.7000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=856
PRIMER_PAIR_9_PENALTY=0.618494
PRIMER_LEFT_9_PENALTY=0.469985
PRIMER_RIGHT_9_PENALTY=0.148509
PRIMER_LEFT_9_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_9_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_9=821,20
PRIMER_RIGHT_9=1531,20
PRIMER_LEFT_9_TM=60.470
PRIMER_RIGHT_9_TM=60.149
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=711
PRIMER_PAIR_10_PENALTY=0.643006
PRIMER_LEFT_10_PENALTY=0.072951
PRIMER_RIGHT_10_PENALTY=0.570054
PRIMER_LEFT_10_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_10_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_10=816,20
PRIMER_RIGHT_10=1607,20
PRIMER_LEFT_10_TM=60.073
PRIMER_RIGHT_10_TM=60.570
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=792
PRIMER_PAIR_11_PENALTY=0.645988
PRIMER_LEFT_11_PENALTY=0.546410
PRIMER_RIGHT_11_PENALTY=0.099578
PRIMER_LEFT_11_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_11_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_11=888,20
PRIMER_RIGHT_11=1756,20
PRIMER_LEFT_11_TM=59.454
PRIMER_RIGHT_11_TM=59.900
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=869
PRIMER_PAIR_12_PENALTY=0.660210
PRIMER_LEFT_12_PENALTY=0.090155
PRIMER_RIGHT_12_PENALTY=0.570054
PRIMER_LEFT_12_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_12_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_12=670,20
PRIMER_RIGHT_12=1607,20
PRIMER_LEFT_12_TM=59.910
PRIMER_RIGHT_12_TM=60.570
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=11.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=938
PRIMER_PAIR_13_PENALTY=0.681386
PRIMER_LEFT_13_PENALTY=0.581808
PRIMER_RIGHT_13_PENALTY=0.099578
PRIMER_LEFT_13_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_13_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_13=873,20
PRIMER_RIGHT_13=1756,20
PRIMER_LEFT_13_TM=60.582
PRIMER_RIGHT_13_TM=59.900
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=5.6000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=884
PRIMER_PAIR_14_PENALTY=0.687201
PRIMER_LEFT_14_PENALTY=0.309369
PRIMER_RIGHT_14_PENALTY=0.377832
PRIMER_LEFT_14_SEQUENCE=TTGGAATGGATTTTGCAGGT
PRIMER_RIGHT_14_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_14=563,20
PRIMER_RIGHT_14=1532,20
PRIMER_LEFT_14_TM=60.309
PRIMER_RIGHT_14_TM=59.622
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=970
PRIMER_PAIR_15_PENALTY=0.694919
PRIMER_LEFT_15_PENALTY=0.546410
PRIMER_RIGHT_15_PENALTY=0.148509
PRIMER_LEFT_15_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_15_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_15=888,20
PRIMER_RIGHT_15=1531,20
PRIMER_LEFT_15_TM=59.454
PRIMER_RIGHT_15_TM=60.149
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=644
PRIMER_PAIR_16_PENALTY=0.730317
PRIMER_LEFT_16_PENALTY=0.581808
PRIMER_RIGHT_16_PENALTY=0.148509
PRIMER_LEFT_16_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_16_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_16=873,20
PRIMER_RIGHT_16=1531,20
PRIMER_LEFT_16_TM=60.582
PRIMER_RIGHT_16_TM=60.149
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.6000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=659
PRIMER_PAIR_17_PENALTY=0.924242
PRIMER_LEFT_17_PENALTY=0.546410
PRIMER_RIGHT_17_PENALTY=0.377832
PRIMER_LEFT_17_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_17_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_17=888,20
PRIMER_RIGHT_17=1532,20
PRIMER_LEFT_17_TM=59.454
PRIMER_RIGHT_17_TM=59.622
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=645
PRIMER_PAIR_18_PENALTY=0.955163
PRIMER_LEFT_18_PENALTY=0.806653
PRIMER_RIGHT_18_PENALTY=0.148509
PRIMER_LEFT_18_SEQUENCE=TTTGGAATGGATTTTGCAGG
PRIMER_RIGHT_18_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_18=562,20
PRIMER_RIGHT_18=1531,20
PRIMER_LEFT_18_TM=60.807
PRIMER_RIGHT_18_TM=60.149
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=970
PRIMER_PAIR_19_PENALTY=0.959640
PRIMER_LEFT_19_PENALTY=0.581808
PRIMER_RIGHT_19_PENALTY=0.377832
PRIMER_LEFT_19_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_19_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_19=873,20
PRIMER_RIGHT_19=1532,20
PRIMER_LEFT_19_TM=60.582
PRIMER_RIGHT_19_TM=59.622
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=660
PRIMER_PAIR_20_PENALTY=1.034004
PRIMER_LEFT_20_PENALTY=0.934426
PRIMER_RIGHT_20_PENALTY=0.099578
PRIMER_LEFT_20_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_20_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_20=885,20
PRIMER_RIGHT_20=1756,20
PRIMER_LEFT_20_TM=60.934
PRIMER_RIGHT_20_TM=59.900
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=872
PRIMER_PAIR_21_PENALTY=1.040039
PRIMER_LEFT_21_PENALTY=0.469985
PRIMER_RIGHT_21_PENALTY=0.570054
PRIMER_LEFT_21_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_21_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_21=821,20
PRIMER_RIGHT_21=1607,20
PRIMER_LEFT_21_TM=60.470
PRIMER_RIGHT_21_TM=60.570
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=787
PRIMER_PAIR_22_PENALTY=1.075195
PRIMER_LEFT_22_PENALTY=0.975617
PRIMER_RIGHT_22_PENALTY=0.099578
PRIMER_LEFT_22_SEQUENCE=CAAATACGAGCCGAGTGAGA
PRIMER_RIGHT_22_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_22=886,20
PRIMER_RIGHT_22=1756,20
PRIMER_LEFT_22_TM=59.024
PRIMER_RIGHT_22_TM=59.900
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=871
PRIMER_PAIR_23_PENALTY=1.082935
PRIMER_LEFT_23_PENALTY=0.934426
PRIMER_RIGHT_23_PENALTY=0.148509
PRIMER_LEFT_23_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_23_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_23=885,20
PRIMER_RIGHT_23=1531,20
PRIMER_LEFT_23_TM=60.934
PRIMER_RIGHT_23_TM=60.149
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=647
PRIMER_PAIR_24_PENALTY=1.106758
PRIMER_LEFT_24_PENALTY=0.072951
PRIMER_RIGHT_24_PENALTY=1.033807
PRIMER_LEFT_24_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_24_SEQUENCE=CGGCCGTTTAAATCCATACT
PRIMER_LEFT_24=816,20
PRIMER_RIGHT_24=1806,20
PRIMER_LEFT_24_TM=60.073
PRIMER_RIGHT_24_TM=58.966
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=5.3000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=991
PRIMER_PAIR_25_PENALTY=1.116465
PRIMER_LEFT_25_PENALTY=0.546410
PRIMER_RIGHT_25_PENALTY=0.570054
PRIMER_LEFT_25_SEQUENCE=AATACGAGCCGAGTGAGAGG
PRIMER_RIGHT_25_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_25=888,20
PRIMER_RIGHT_25=1607,20
PRIMER_LEFT_25_TM=59.454
PRIMER_RIGHT_25_TM=60.570
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=720
PRIMER_PAIR_26_PENALTY=1.122366
PRIMER_LEFT_26_PENALTY=1.022788
PRIMER_RIGHT_26_PENALTY=0.099578
PRIMER_LEFT_26_SEQUENCE=CAAATACGAGCCGAGTGAGAG
PRIMER_RIGHT_26_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_26=886,21
PRIMER_RIGHT_26=1756,20
PRIMER_LEFT_26_TM=60.023
PRIMER_RIGHT_26_TM=59.900
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=871
PRIMER_PAIR_27_PENALTY=1.124126
PRIMER_LEFT_27_PENALTY=0.975617
PRIMER_RIGHT_27_PENALTY=0.148509
PRIMER_LEFT_27_SEQUENCE=CAAATACGAGCCGAGTGAGA
PRIMER_RIGHT_27_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_27=886,20
PRIMER_RIGHT_27=1531,20
PRIMER_LEFT_27_TM=59.024
PRIMER_RIGHT_27_TM=60.149
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=646
PRIMER_PAIR_28_PENALTY=1.151862
PRIMER_LEFT_28_PENALTY=0.581808
PRIMER_RIGHT_28_PENALTY=0.570054
PRIMER_LEFT_28_SEQUENCE=AGCTCCGGAAAGGCAAATAC
PRIMER_RIGHT_28_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_28=873,20
PRIMER_RIGHT_28=1607,20
PRIMER_LEFT_28_TM=60.582
PRIMER_RIGHT_28_TM=60.570
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=5.6000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=735
PRIMER_PAIR_29_PENALTY=1.171297
PRIMER_LEFT_29_PENALTY=1.022788
PRIMER_RIGHT_29_PENALTY=0.148509
PRIMER_LEFT_29_SEQUENCE=CAAATACGAGCCGAGTGAGAG
PRIMER_RIGHT_29_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_29=886,21
PRIMER_RIGHT_29=1531,20
PRIMER_LEFT_29_TM=60.023
PRIMER_RIGHT_29_TM=60.149
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=646
PRIMER_PAIR_30_PENALTY=1.184485
PRIMER_LEFT_30_PENALTY=0.806653
PRIMER_RIGHT_30_PENALTY=0.377832
PRIMER_LEFT_30_SEQUENCE=TTTGGAATGGATTTTGCAGG
PRIMER_RIGHT_30_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_30=562,20
PRIMER_RIGHT_30=1532,20
PRIMER_LEFT_30_TM=60.807
PRIMER_RIGHT_30_TM=59.622
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=971
PRIMER_PAIR_31_PENALTY=1.202852
PRIMER_LEFT_31_PENALTY=1.054343
PRIMER_RIGHT_31_PENALTY=0.148509
PRIMER_LEFT_31_SEQUENCE=TGGAATGGATTTTGCAGGTAG
PRIMER_RIGHT_31_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_31=564,21
PRIMER_RIGHT_31=1531,20
PRIMER_LEFT_31_TM=59.946
PRIMER_RIGHT_31_TM=60.149
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=968
PRIMER_PAIR_32_PENALTY=1.232417
PRIMER_LEFT_32_PENALTY=0.072951
PRIMER_RIGHT_32_PENALTY=1.159466
PRIMER_LEFT_32_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_32_SEQUENCE=TCAGATTCTCAACATCCGCC
PRIMER_LEFT_32=816,20
PRIMER_RIGHT_32=1759,20
PRIMER_LEFT_32_TM=60.073
PRIMER_RIGHT_32_TM=61.159
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=12.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=944
PRIMER_PAIR_33_PENALTY=1.235607
PRIMER_LEFT_33_PENALTY=1.136029
PRIMER_RIGHT_33_PENALTY=0.099578
PRIMER_LEFT_33_SEQUENCE=AAGGCAAATACGAGCCGAGT
PRIMER_RIGHT_33_SEQUENCE=GATTCTCAACATCCGCCATT
PRIMER_LEFT_33=882,20
PRIMER_RIGHT_33=1756,20
PRIMER_LEFT_33_TM=61.136
PRIMER_RIGHT_33_TM=59.900
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=875
PRIMER_PAIR_34_PENALTY=1.284538
PRIMER_LEFT_34_PENALTY=1.136029
PRIMER_RIGHT_34_PENALTY=0.148509
PRIMER_LEFT_34_SEQUENCE=AAGGCAAATACGAGCCGAGT
PRIMER_RIGHT_34_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_34=882,20
PRIMER_RIGHT_34=1531,20
PRIMER_LEFT_34_TM=61.136
PRIMER_RIGHT_34_TM=60.149
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=650
PRIMER_PAIR_35_PENALTY=1.290653
PRIMER_LEFT_35_PENALTY=0.072951
PRIMER_RIGHT_35_PENALTY=1.217702
PRIMER_LEFT_35_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_35_SEQUENCE=CTACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_35=816,20
PRIMER_RIGHT_35=1534,21
PRIMER_LEFT_35_TM=60.073
PRIMER_RIGHT_35_TM=59.782
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=719
PRIMER_PAIR_36_PENALTY=1.300209
PRIMER_LEFT_36_PENALTY=0.072951
PRIMER_RIGHT_36_PENALTY=1.227257
PRIMER_LEFT_36_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_36_SEQUENCE=TACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_36=816,20
PRIMER_RIGHT_36=1533,20
PRIMER_LEFT_36_TM=60.073
PRIMER_RIGHT_36_TM=58.773
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=718
PRIMER_PAIR_37_PENALTY=1.307857
PRIMER_LEFT_37_PENALTY=0.090155
PRIMER_RIGHT_37_PENALTY=1.217702
PRIMER_LEFT_37_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_37_SEQUENCE=CTACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_37=670,20
PRIMER_RIGHT_37=1534,21
PRIMER_LEFT_37_TM=59.910
PRIMER_RIGHT_37_TM=59.782
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=11.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=865
PRIMER_PAIR_38_PENALTY=1.312258
PRIMER_LEFT_38_PENALTY=0.934426
PRIMER_RIGHT_38_PENALTY=0.377832
PRIMER_LEFT_38_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_38_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_38=885,20
PRIMER_RIGHT_38=1532,20
PRIMER_LEFT_38_TM=60.934
PRIMER_RIGHT_38_TM=59.622
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=7.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=648
PRIMER_PAIR_39_PENALTY=1.317413
PRIMER_LEFT_39_PENALTY=0.090155
PRIMER_RIGHT_39_PENALTY=1.227257
PRIMER_LEFT_39_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_39_SEQUENCE=TACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_39=670,20
PRIMER_RIGHT_39=1533,20
PRIMER_LEFT_39_TM=59.910
PRIMER_RIGHT_39_TM=58.773
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=11.7000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=864
PRIMER_PAIR_40_PENALTY=1.353449
PRIMER_LEFT_40_PENALTY=0.975617
PRIMER_RIGHT_40_PENALTY=0.377832
PRIMER_LEFT_40_SEQUENCE=CAAATACGAGCCGAGTGAGA
PRIMER_RIGHT_40_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_40=886,20
PRIMER_RIGHT_40=1532,20
PRIMER_LEFT_40_TM=59.024
PRIMER_RIGHT_40_TM=59.622
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=647
PRIMER_PAIR_41_PENALTY=1.364576
PRIMER_LEFT_41_PENALTY=1.216066
PRIMER_RIGHT_41_PENALTY=0.148509
PRIMER_LEFT_41_SEQUENCE=TAAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_41_SEQUENCE=CTCGTCCGTCTTGGTTTGTT
PRIMER_LEFT_41=676,21
PRIMER_RIGHT_41=1531,20
PRIMER_LEFT_41_TM=60.216
PRIMER_RIGHT_41_TM=60.149
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.7000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=856
PRIMER_PAIR_42_PENALTY=1.400620
PRIMER_LEFT_42_PENALTY=1.022788
PRIMER_RIGHT_42_PENALTY=0.377832
PRIMER_LEFT_42_SEQUENCE=CAAATACGAGCCGAGTGAGAG
PRIMER_RIGHT_42_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_42=886,21
PRIMER_RIGHT_42=1532,20
PRIMER_LEFT_42_TM=60.023
PRIMER_RIGHT_42_TM=59.622
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=647
PRIMER_PAIR_43_PENALTY=1.425367
PRIMER_LEFT_43_PENALTY=0.072951
PRIMER_RIGHT_43_PENALTY=1.352415
PRIMER_LEFT_43_SEQUENCE=AGAACGTGATGATCGAAGGG
PRIMER_RIGHT_43_SEQUENCE=TACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_43=816,20
PRIMER_RIGHT_43=1533,21
PRIMER_LEFT_43_TM=60.073
PRIMER_RIGHT_43_TM=59.648
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=718
PRIMER_PAIR_44_PENALTY=1.431207
PRIMER_LEFT_44_PENALTY=0.213506
PRIMER_RIGHT_44_PENALTY=1.217702
PRIMER_LEFT_44_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_44_SEQUENCE=CTACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_44=677,20
PRIMER_RIGHT_44=1534,21
PRIMER_LEFT_44_TM=60.214
PRIMER_RIGHT_44_TM=59.782
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.7000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=858
PRIMER_PAIR_45_PENALTY=1.432175
PRIMER_LEFT_45_PENALTY=1.054343
PRIMER_RIGHT_45_PENALTY=0.377832
PRIMER_LEFT_45_SEQUENCE=TGGAATGGATTTTGCAGGTAG
PRIMER_RIGHT_45_SEQUENCE=ACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_45=564,21
PRIMER_RIGHT_45=1532,20
PRIMER_LEFT_45_TM=59.946
PRIMER_RIGHT_45_TM=59.622
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=969
PRIMER_PAIR_46_PENALTY=1.440763
PRIMER_LEFT_46_PENALTY=0.213506
PRIMER_RIGHT_46_PENALTY=1.227257
PRIMER_LEFT_46_SEQUENCE=AAATCTCTCGGCATTCGTTG
PRIMER_RIGHT_46_SEQUENCE=TACTCGTCCGTCTTGGTTTG
PRIMER_LEFT_46=677,20
PRIMER_RIGHT_46=1533,20
PRIMER_LEFT_46_TM=60.214
PRIMER_RIGHT_46_TM=58.773
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=857
PRIMER_PAIR_47_PENALTY=1.442571
PRIMER_LEFT_47_PENALTY=0.090155
PRIMER_RIGHT_47_PENALTY=1.352415
PRIMER_LEFT_47_SEQUENCE=TCCTCCTAAATCTCTCGGCA
PRIMER_RIGHT_47_SEQUENCE=TACTCGTCCGTCTTGGTTTGT
PRIMER_LEFT_47=670,20
PRIMER_RIGHT_47=1533,21
PRIMER_LEFT_47_TM=59.910
PRIMER_RIGHT_47_TM=59.648
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=11.7000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=864
PRIMER_PAIR_48_PENALTY=1.503792
PRIMER_LEFT_48_PENALTY=0.469985
PRIMER_RIGHT_48_PENALTY=1.033807
PRIMER_LEFT_48_SEQUENCE=GTGATGATCGAAGGGCAAAC
PRIMER_RIGHT_48_SEQUENCE=CGGCCGTTTAAATCCATACT
PRIMER_LEFT_48=821,20
PRIMER_RIGHT_48=1806,20
PRIMER_LEFT_48_TM=60.470
PRIMER_RIGHT_48_TM=58.966
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=5.3000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=986
PRIMER_PAIR_49_PENALTY=1.504480
PRIMER_LEFT_49_PENALTY=0.934426
PRIMER_RIGHT_49_PENALTY=0.570054
PRIMER_LEFT_49_SEQUENCE=GCAAATACGAGCCGAGTGAG
PRIMER_RIGHT_49_SEQUENCE=TCCACACGAATGTGACAAGG
PRIMER_LEFT_49=885,20
PRIMER_RIGHT_49=1607,20
PRIMER_LEFT_49_TM=60.934
PRIMER_RIGHT_49_TM=60.570
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=723
=
SEQUENCE_ID=MAGIC.329_4_8803005_8803075_ws-0_ler-0_510134_145019_TRUE
SEQUENCE_TARGET=950,170
SEQUENCE_TEMPLATE=CNAAAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAACGACTTGCCAATTCACCTNCGTACTACTAGTTAATCGACNAGA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17510520_17510555_bur-0_can-0_9632_3957_TRUE
SEQUENCE_TARGET=950,135
SEQUENCE_TEMPLATE=TTAAAACGAATATGTTGTATCATCTTTAAAGATTTTTTCTGNTTTGGTTTGAAACTTTATGGTCAAACCCGTTGTTGATTGACTTATCTTCTTTGTGTAAGTNGCTTGAGAGAGTTGATATTGGAGGCATAGGAGAGAAGCATTGTCAAACAAGAACAAACCGATATCAACATTTATAGTTCCAATGGGATTCTTTTGATCAATTTGAAACATTGCNACTTTCCGACTTTCCTTTCCTGTCTACTTTTTTTGTGCACNATAGAGTTAGAAACAACTTCTATCATCTGCCAGAAGCATGATAGTGATGCCACTCTCTTTATCCATGGCTTAAGGGATCAGTAACCTGATGCACCTTACGAGACTCTGAGCTTGAGTACTGTCCCACGGCTCGGGCATAAGGAATTGATTGTTTCCCGGCTGCAAGCGCATTCACATTTTCAACTAAATACTGTCTTGGGAGCCTCCCGACCACATTACCTTCTTCGTTCCCTTCTCTATCGAGGAAGGCGAAATGAGGAATACCCTCAACACCAAACTCATCCAACTCTTGCTCCCATTTCGTGTTGTCCACATTTAGCATCACAAAGTTAACTTTGTCCCTGCGAAATCACAACACCACAAGTAAACATCAACTTTAGCCTACAAAACGTCAAATTAGTGTCTAAAAAGCTCCATGTTCCATTAAACTAACATGAGATTAATGGTTTGCCTCTGCAACTAAAATCTCAAAGCTCTACCTTTTTCATNAGNGTCTATCTACATAGCCAACAATCCAATCCTNAAGATAGTTCCCTAATCCATTAAACCAACACAAAATAAGAACTCTTCTGCAACTAGAATCTTTAAAGCTCTANCTAGTTCATTAGCTAAATCCATTCCAAGACAAGATTCCTTAATCCATGTTTTTCGAAGCTCTAGCAATGCTCANCTTAATCTCCAGACAAGTTCCCTAATCCGTTCTGCAACTAGAAAGCNCTAGCTTGTCTTTCATCTATATATANAGGCTTCATTTTGAAAGGAGATTGATTTTGATCANAAATCACATAAAAGACGATNCATTACTTGTATTGCTGNTCGATTTTATAAACATCAGGAGCTAGTTCTCGACAAACTTCACACCAATCTGCATAGAACTCAACAACAGTCGGCTTCCCATTCGATAANGCCTAAATATCCATTCACAAAACTCATTAAGCATTCTATGAGAATAGAAACACAGATGAAGAATCCAAAAACAAACCTCCTCGTAAGGCAACGCAGAAGCGGTTAAATCCTTCAAAGAAATCCCAAAATCGAGCCTTGTCGATACAAATAACGACAAAGCCGCGATTACAGTAACAGCCGCAACTCTTCTATTGATATCCTTATTGGGAGANTCAGGGAATCCCNNNGAAGAAGAAGAAGAAGAGTCAGCAACAGATGATGATGATTCCACTTCCTTCGATGCAGATGAAGTTTCACCATTATCTATTACCAATTTCTCCTGAATCAGGTAAAGGCTANAACTTTAGCTAATTTNCCTTCGACCCATTTCAAAAAGAATCGAAATTTTCACCTGTTTGGTTTCGGAAGATTCTGGGTTTGGTTTGCAACGAACAGCGCGAAATCGTGGAGCTCCTGTTTGGGTAACGAAGAAAGATTGAAGATTTCGAGGACTGAGATTGAATCCATGACTAGATGGAANGTTTAANGAAAATACTAAGCGAGCCATCGTCGNGAGAGAGTGAGAGAGATGACAAAACTGCTACTCATCAGATCGTTATTATCGANGGAGTAGTAGCTTCTTCGTCTNACCAAGCTATTAGAGCCACACGCTTTTTGTTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.132456
PRIMER_LEFT_0_PENALTY=0.051345
PRIMER_RIGHT_0_PENALTY=0.081111
PRIMER_LEFT_0_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_0_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_0=491,20
PRIMER_RIGHT_0=1246,20
PRIMER_LEFT_0_TM=60.051
PRIMER_RIGHT_0_TM=60.081
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=756
PRIMER_PAIR_1_PENALTY=0.132456
PRIMER_LEFT_1_PENALTY=0.051345
PRIMER_RIGHT_1_PENALTY=0.081111
PRIMER_LEFT_1_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_1_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_1=490,20
PRIMER_RIGHT_1=1246,20
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=757
PRIMER_PAIR_2_PENALTY=0.141925
PRIMER_LEFT_2_PENALTY=0.051345
PRIMER_RIGHT_2_PENALTY=0.090580
PRIMER_LEFT_2_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_2_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_2=491,20
PRIMER_RIGHT_2=1300,20
PRIMER_LEFT_2_TM=60.051
PRIMER_RIGHT_2_TM=59.909
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=810
PRIMER_PAIR_3_PENALTY=0.141925
PRIMER_LEFT_3_PENALTY=0.051345
PRIMER_RIGHT_3_PENALTY=0.090580
PRIMER_LEFT_3_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_3_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_3=490,20
PRIMER_RIGHT_3=1300,20
PRIMER_LEFT_3_TM=60.051
PRIMER_RIGHT_3_TM=59.909
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=811
PRIMER_PAIR_4_PENALTY=0.189459
PRIMER_LEFT_4_PENALTY=0.051345
PRIMER_RIGHT_4_PENALTY=0.138114
PRIMER_LEFT_4_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_4_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_4=491,20
PRIMER_RIGHT_4=1326,20
PRIMER_LEFT_4_TM=60.051
PRIMER_RIGHT_4_TM=60.138
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=836
PRIMER_PAIR_5_PENALTY=0.189459
PRIMER_LEFT_5_PENALTY=0.051345
PRIMER_RIGHT_5_PENALTY=0.138114
PRIMER_LEFT_5_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_5_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_5=490,20
PRIMER_RIGHT_5=1326,20
PRIMER_LEFT_5_TM=60.051
PRIMER_RIGHT_5_TM=60.138
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=11.4000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=837
PRIMER_PAIR_6_PENALTY=0.203871
PRIMER_LEFT_6_PENALTY=0.159749
PRIMER_RIGHT_6_PENALTY=0.044122
PRIMER_LEFT_6_SEQUENCE=CTGCGAAATCACAACACCAC
PRIMER_RIGHT_6_SEQUENCE=CCAGAATCTTCCGAAACCAA
PRIMER_LEFT_6=599,20
PRIMER_RIGHT_6=1578,20
PRIMER_LEFT_6_TM=60.160
PRIMER_RIGHT_6_TM=60.044
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=980
PRIMER_PAIR_7_PENALTY=0.250125
PRIMER_LEFT_7_PENALTY=0.159749
PRIMER_RIGHT_7_PENALTY=0.090376
PRIMER_LEFT_7_SEQUENCE=CTGCGAAATCACAACACCAC
PRIMER_RIGHT_7_SEQUENCE=TTTTTGAAATGGGTCGAAGG
PRIMER_LEFT_7=599,20
PRIMER_RIGHT_7=1538,20
PRIMER_LEFT_7_TM=60.160
PRIMER_RIGHT_7_TM=59.910
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=940
PRIMER_PAIR_8_PENALTY=0.269319
PRIMER_LEFT_8_PENALTY=0.051345
PRIMER_RIGHT_8_PENALTY=0.217974
PRIMER_LEFT_8_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_8_SEQUENCE=ATCTGCATCGAAGGAAGTGG
PRIMER_LEFT_8=491,20
PRIMER_RIGHT_8=1450,20
PRIMER_LEFT_8_TM=60.051
PRIMER_RIGHT_8_TM=60.218
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.2000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=960
PRIMER_PAIR_9_PENALTY=0.269319
PRIMER_LEFT_9_PENALTY=0.051345
PRIMER_RIGHT_9_PENALTY=0.217974
PRIMER_LEFT_9_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_9_SEQUENCE=ATCTGCATCGAAGGAAGTGG
PRIMER_LEFT_9=490,20
PRIMER_RIGHT_9=1450,20
PRIMER_LEFT_9_TM=60.051
PRIMER_RIGHT_9_TM=60.218
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=11.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=961
PRIMER_PAIR_10_PENALTY=0.278014
PRIMER_LEFT_10_PENALTY=0.196903
PRIMER_RIGHT_10_PENALTY=0.081111
PRIMER_LEFT_10_SEQUENCE=CTCATCCAACTCTTGCTCCC
PRIMER_RIGHT_10_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_10=535,20
PRIMER_RIGHT_10=1246,20
PRIMER_LEFT_10_TM=59.803
PRIMER_RIGHT_10_TM=60.081
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=712
PRIMER_PAIR_11_PENALTY=0.280358
PRIMER_LEFT_11_PENALTY=0.199246
PRIMER_RIGHT_11_PENALTY=0.081111
PRIMER_LEFT_11_SEQUENCE=CTATCGAGGAAGGCGAAATG
PRIMER_RIGHT_11_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_11=494,20
PRIMER_RIGHT_11=1246,20
PRIMER_LEFT_11_TM=59.801
PRIMER_RIGHT_11_TM=60.081
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.2000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=753
PRIMER_PAIR_12_PENALTY=0.287483
PRIMER_LEFT_12_PENALTY=0.196903
PRIMER_RIGHT_12_PENALTY=0.090580
PRIMER_LEFT_12_SEQUENCE=CTCATCCAACTCTTGCTCCC
PRIMER_RIGHT_12_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_12=535,20
PRIMER_RIGHT_12=1300,20
PRIMER_LEFT_12_TM=59.803
PRIMER_RIGHT_12_TM=59.909
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=766
PRIMER_PAIR_13_PENALTY=0.289826
PRIMER_LEFT_13_PENALTY=0.199246
PRIMER_RIGHT_13_PENALTY=0.090580
PRIMER_LEFT_13_SEQUENCE=CTATCGAGGAAGGCGAAATG
PRIMER_RIGHT_13_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_13=494,20
PRIMER_RIGHT_13=1300,20
PRIMER_LEFT_13_TM=59.801
PRIMER_RIGHT_13_TM=59.909
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.2000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=807
PRIMER_PAIR_14_PENALTY=0.292377
PRIMER_LEFT_14_PENALTY=0.051345
PRIMER_RIGHT_14_PENALTY=0.241032
PRIMER_LEFT_14_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_14_SEQUENCE=TTGCCTTACGAGGAGGTTTG
PRIMER_LEFT_14=491,20
PRIMER_RIGHT_14=1254,20
PRIMER_LEFT_14_TM=60.051
PRIMER_RIGHT_14_TM=60.241
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=10.2000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=764
PRIMER_PAIR_15_PENALTY=0.292377
PRIMER_LEFT_15_PENALTY=0.051345
PRIMER_RIGHT_15_PENALTY=0.241032
PRIMER_LEFT_15_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_15_SEQUENCE=TTGCCTTACGAGGAGGTTTG
PRIMER_LEFT_15=490,20
PRIMER_RIGHT_15=1254,20
PRIMER_LEFT_15_TM=60.051
PRIMER_RIGHT_15_TM=60.241
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=765
PRIMER_PAIR_16_PENALTY=0.297863
PRIMER_LEFT_16_PENALTY=0.159749
PRIMER_RIGHT_16_PENALTY=0.138114
PRIMER_LEFT_16_SEQUENCE=CTGCGAAATCACAACACCAC
PRIMER_RIGHT_16_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_16=599,20
PRIMER_RIGHT_16=1326,20
PRIMER_LEFT_16_TM=60.160
PRIMER_RIGHT_16_TM=60.138
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=728
PRIMER_PAIR_17_PENALTY=0.303826
PRIMER_LEFT_17_PENALTY=0.259704
PRIMER_RIGHT_17_PENALTY=0.044122
PRIMER_LEFT_17_SEQUENCE=AATGGTTTGCCTCTGCAACT
PRIMER_RIGHT_17_SEQUENCE=CCAGAATCTTCCGAAACCAA
PRIMER_LEFT_17=699,20
PRIMER_RIGHT_17=1578,20
PRIMER_LEFT_17_TM=59.740
PRIMER_RIGHT_17_TM=60.044
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=880
PRIMER_PAIR_18_PENALTY=0.315645
PRIMER_LEFT_18_PENALTY=0.051345
PRIMER_RIGHT_18_PENALTY=0.264300
PRIMER_LEFT_18_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_18_SEQUENCE=ATTTAACCGCTTCTGCGTTG
PRIMER_LEFT_18=491,20
PRIMER_RIGHT_18=1271,20
PRIMER_LEFT_18_TM=60.051
PRIMER_RIGHT_18_TM=60.264
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=10.2000
PRIMER_RIGHT_18_END_STABILITY=8.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=781
PRIMER_PAIR_19_PENALTY=0.315645
PRIMER_LEFT_19_PENALTY=0.051345
PRIMER_RIGHT_19_PENALTY=0.264300
PRIMER_LEFT_19_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_19_SEQUENCE=ATTTAACCGCTTCTGCGTTG
PRIMER_LEFT_19=490,20
PRIMER_RIGHT_19=1271,20
PRIMER_LEFT_19_TM=60.051
PRIMER_RIGHT_19_TM=60.264
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=11.4000
PRIMER_RIGHT_19_END_STABILITY=8.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=782
PRIMER_PAIR_20_PENALTY=0.317060
PRIMER_LEFT_20_PENALTY=0.051345
PRIMER_RIGHT_20_PENALTY=0.265715
PRIMER_LEFT_20_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_20_SEQUENCE=TGCCTTACGAGGAGGTTTGT
PRIMER_LEFT_20=491,20
PRIMER_RIGHT_20=1253,20
PRIMER_LEFT_20_TM=60.051
PRIMER_RIGHT_20_TM=59.734
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=10.2000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=763
PRIMER_PAIR_21_PENALTY=0.317060
PRIMER_LEFT_21_PENALTY=0.051345
PRIMER_RIGHT_21_PENALTY=0.265715
PRIMER_LEFT_21_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_21_SEQUENCE=TGCCTTACGAGGAGGTTTGT
PRIMER_LEFT_21=490,20
PRIMER_RIGHT_21=1253,20
PRIMER_LEFT_21_TM=60.051
PRIMER_RIGHT_21_TM=59.734
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=11.4000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=764
PRIMER_PAIR_22_PENALTY=0.329674
PRIMER_LEFT_22_PENALTY=0.051345
PRIMER_RIGHT_22_PENALTY=0.278329
PRIMER_LEFT_22_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_22_SEQUENCE=ACTGTAATCGCGGCTTTGTC
PRIMER_LEFT_22=491,20
PRIMER_RIGHT_22=1335,20
PRIMER_LEFT_22_TM=60.051
PRIMER_RIGHT_22_TM=60.278
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=10.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=845
PRIMER_PAIR_23_PENALTY=0.329674
PRIMER_LEFT_23_PENALTY=0.051345
PRIMER_RIGHT_23_PENALTY=0.278329
PRIMER_LEFT_23_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_23_SEQUENCE=ACTGTAATCGCGGCTTTGTC
PRIMER_LEFT_23=490,20
PRIMER_RIGHT_23=1335,20
PRIMER_LEFT_23_TM=60.051
PRIMER_RIGHT_23_TM=60.278
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=11.4000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=846
PRIMER_PAIR_24_PENALTY=0.335017
PRIMER_LEFT_24_PENALTY=0.196903
PRIMER_RIGHT_24_PENALTY=0.138114
PRIMER_LEFT_24_SEQUENCE=CTCATCCAACTCTTGCTCCC
PRIMER_RIGHT_24_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_24=535,20
PRIMER_RIGHT_24=1326,20
PRIMER_LEFT_24_TM=59.803
PRIMER_RIGHT_24_TM=60.138
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=792
PRIMER_PAIR_25_PENALTY=0.335724
PRIMER_LEFT_25_PENALTY=0.254613
PRIMER_RIGHT_25_PENALTY=0.081111
PRIMER_LEFT_25_SEQUENCE=CTCTTGCTCCCATTTCGTGT
PRIMER_RIGHT_25_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_25=544,20
PRIMER_RIGHT_25=1246,20
PRIMER_LEFT_25_TM=60.255
PRIMER_RIGHT_25_TM=60.081
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=703
PRIMER_PAIR_26_PENALTY=0.335724
PRIMER_LEFT_26_PENALTY=0.254613
PRIMER_RIGHT_26_PENALTY=0.081111
PRIMER_LEFT_26_SEQUENCE=ACTCTTGCTCCCATTTCGTG
PRIMER_RIGHT_26_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_26=543,20
PRIMER_RIGHT_26=1246,20
PRIMER_LEFT_26_TM=60.255
PRIMER_RIGHT_26_TM=60.081
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=704
PRIMER_PAIR_27_PENALTY=0.337360
PRIMER_LEFT_27_PENALTY=0.199246
PRIMER_RIGHT_27_PENALTY=0.138114
PRIMER_LEFT_27_SEQUENCE=CTATCGAGGAAGGCGAAATG
PRIMER_RIGHT_27_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_27=494,20
PRIMER_RIGHT_27=1326,20
PRIMER_LEFT_27_TM=59.801
PRIMER_RIGHT_27_TM=60.138
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.2000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=833
PRIMER_PAIR_28_PENALTY=0.340815
PRIMER_LEFT_28_PENALTY=0.259704
PRIMER_RIGHT_28_PENALTY=0.081111
PRIMER_LEFT_28_SEQUENCE=AATGGTTTGCCTCTGCAACT
PRIMER_RIGHT_28_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_28=699,20
PRIMER_RIGHT_28=1246,20
PRIMER_LEFT_28_TM=59.740
PRIMER_RIGHT_28_TM=60.081
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=548
PRIMER_PAIR_29_PENALTY=0.344989
PRIMER_LEFT_29_PENALTY=0.254613
PRIMER_RIGHT_29_PENALTY=0.090376
PRIMER_LEFT_29_SEQUENCE=CTCTTGCTCCCATTTCGTGT
PRIMER_RIGHT_29_SEQUENCE=TTTTTGAAATGGGTCGAAGG
PRIMER_LEFT_29=544,20
PRIMER_RIGHT_29=1538,20
PRIMER_LEFT_29_TM=60.255
PRIMER_RIGHT_29_TM=59.910
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=8.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=995
PRIMER_PAIR_30_PENALTY=0.345193
PRIMER_LEFT_30_PENALTY=0.254613
PRIMER_RIGHT_30_PENALTY=0.090580
PRIMER_LEFT_30_SEQUENCE=CTCTTGCTCCCATTTCGTGT
PRIMER_RIGHT_30_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_30=544,20
PRIMER_RIGHT_30=1300,20
PRIMER_LEFT_30_TM=60.255
PRIMER_RIGHT_30_TM=59.909
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=757
PRIMER_PAIR_31_PENALTY=0.345193
PRIMER_LEFT_31_PENALTY=0.254613
PRIMER_RIGHT_31_PENALTY=0.090580
PRIMER_LEFT_31_SEQUENCE=ACTCTTGCTCCCATTTCGTG
PRIMER_RIGHT_31_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_31=543,20
PRIMER_RIGHT_31=1300,20
PRIMER_LEFT_31_TM=60.255
PRIMER_RIGHT_31_TM=59.909
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=758
PRIMER_PAIR_32_PENALTY=0.350079
PRIMER_LEFT_32_PENALTY=0.259704
PRIMER_RIGHT_32_PENALTY=0.090376
PRIMER_LEFT_32_SEQUENCE=AATGGTTTGCCTCTGCAACT
PRIMER_RIGHT_32_SEQUENCE=TTTTTGAAATGGGTCGAAGG
PRIMER_LEFT_32=699,20
PRIMER_RIGHT_32=1538,20
PRIMER_LEFT_32_TM=59.740
PRIMER_RIGHT_32_TM=59.910
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=840
PRIMER_PAIR_33_PENALTY=0.350284
PRIMER_LEFT_33_PENALTY=0.259704
PRIMER_RIGHT_33_PENALTY=0.090580
PRIMER_LEFT_33_SEQUENCE=AATGGTTTGCCTCTGCAACT
PRIMER_RIGHT_33_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_33=699,20
PRIMER_RIGHT_33=1300,20
PRIMER_LEFT_33_TM=59.740
PRIMER_RIGHT_33_TM=59.909
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=602
PRIMER_PAIR_34_PENALTY=0.392727
PRIMER_LEFT_34_PENALTY=0.254613
PRIMER_RIGHT_34_PENALTY=0.138114
PRIMER_LEFT_34_SEQUENCE=CTCTTGCTCCCATTTCGTGT
PRIMER_RIGHT_34_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_34=544,20
PRIMER_RIGHT_34=1326,20
PRIMER_LEFT_34_TM=60.255
PRIMER_RIGHT_34_TM=60.138
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=2.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=783
PRIMER_PAIR_35_PENALTY=0.392727
PRIMER_LEFT_35_PENALTY=0.254613
PRIMER_RIGHT_35_PENALTY=0.138114
PRIMER_LEFT_35_SEQUENCE=ACTCTTGCTCCCATTTCGTG
PRIMER_RIGHT_35_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_35=543,20
PRIMER_RIGHT_35=1326,20
PRIMER_LEFT_35_TM=60.255
PRIMER_RIGHT_35_TM=60.138
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.4000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=784
PRIMER_PAIR_36_PENALTY=0.397818
PRIMER_LEFT_36_PENALTY=0.259704
PRIMER_RIGHT_36_PENALTY=0.138114
PRIMER_LEFT_36_SEQUENCE=AATGGTTTGCCTCTGCAACT
PRIMER_RIGHT_36_SEQUENCE=GCGGCTTTGTCGTTATTTGT
PRIMER_LEFT_36=699,20
PRIMER_RIGHT_36=1326,20
PRIMER_LEFT_36_TM=59.740
PRIMER_RIGHT_36_TM=60.138
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=628
PRIMER_PAIR_37_PENALTY=0.410661
PRIMER_LEFT_37_PENALTY=0.329550
PRIMER_RIGHT_37_PENALTY=0.081111
PRIMER_LEFT_37_SEQUENCE=CCAACTCTTGCTCCCATTTC
PRIMER_RIGHT_37_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_37=540,20
PRIMER_RIGHT_37=1246,20
PRIMER_LEFT_37_TM=59.670
PRIMER_RIGHT_37_TM=60.081
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=707
PRIMER_PAIR_38_PENALTY=0.410661
PRIMER_LEFT_38_PENALTY=0.329550
PRIMER_RIGHT_38_PENALTY=0.081111
PRIMER_LEFT_38_SEQUENCE=TCCAACTCTTGCTCCCATTT
PRIMER_RIGHT_38_SEQUENCE=CGAGGAGGTTTGTTTTTGGA
PRIMER_LEFT_38=539,20
PRIMER_RIGHT_38=1246,20
PRIMER_LEFT_38_TM=59.670
PRIMER_RIGHT_38_TM=60.081
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.2000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=708
PRIMER_PAIR_39_PENALTY=0.414877
PRIMER_LEFT_39_PENALTY=0.196903
PRIMER_RIGHT_39_PENALTY=0.217974
PRIMER_LEFT_39_SEQUENCE=CTCATCCAACTCTTGCTCCC
PRIMER_RIGHT_39_SEQUENCE=ATCTGCATCGAAGGAAGTGG
PRIMER_LEFT_39=535,20
PRIMER_RIGHT_39=1450,20
PRIMER_LEFT_39_TM=59.803
PRIMER_RIGHT_39_TM=60.218
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=916
PRIMER_PAIR_40_PENALTY=0.417220
PRIMER_LEFT_40_PENALTY=0.199246
PRIMER_RIGHT_40_PENALTY=0.217974
PRIMER_LEFT_40_SEQUENCE=CTATCGAGGAAGGCGAAATG
PRIMER_RIGHT_40_SEQUENCE=ATCTGCATCGAAGGAAGTGG
PRIMER_LEFT_40=494,20
PRIMER_RIGHT_40=1450,20
PRIMER_LEFT_40_TM=59.801
PRIMER_RIGHT_40_TM=60.218
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.2000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=957
PRIMER_PAIR_41_PENALTY=0.420130
PRIMER_LEFT_41_PENALTY=0.329550
PRIMER_RIGHT_41_PENALTY=0.090580
PRIMER_LEFT_41_SEQUENCE=CCAACTCTTGCTCCCATTTC
PRIMER_RIGHT_41_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_41=540,20
PRIMER_RIGHT_41=1300,20
PRIMER_LEFT_41_TM=59.670
PRIMER_RIGHT_41_TM=59.909
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=761
PRIMER_PAIR_42_PENALTY=0.420130
PRIMER_LEFT_42_PENALTY=0.329550
PRIMER_RIGHT_42_PENALTY=0.090580
PRIMER_LEFT_42_SEQUENCE=TCCAACTCTTGCTCCCATTT
PRIMER_RIGHT_42_SEQUENCE=AAGGCTCGATTTTGGGATTT
PRIMER_LEFT_42=539,20
PRIMER_RIGHT_42=1300,20
PRIMER_LEFT_42_TM=59.670
PRIMER_RIGHT_42_TM=59.909
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.2000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=762
PRIMER_PAIR_43_PENALTY=0.424049
PRIMER_LEFT_43_PENALTY=0.159749
PRIMER_RIGHT_43_PENALTY=0.264300
PRIMER_LEFT_43_SEQUENCE=CTGCGAAATCACAACACCAC
PRIMER_RIGHT_43_SEQUENCE=ATTTAACCGCTTCTGCGTTG
PRIMER_LEFT_43=599,20
PRIMER_RIGHT_43=1271,20
PRIMER_LEFT_43_TM=60.160
PRIMER_RIGHT_43_TM=60.264
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=8.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=673
PRIMER_PAIR_44_PENALTY=0.437935
PRIMER_LEFT_44_PENALTY=0.196903
PRIMER_RIGHT_44_PENALTY=0.241032
PRIMER_LEFT_44_SEQUENCE=CTCATCCAACTCTTGCTCCC
PRIMER_RIGHT_44_SEQUENCE=TTGCCTTACGAGGAGGTTTG
PRIMER_LEFT_44=535,20
PRIMER_RIGHT_44=1254,20
PRIMER_LEFT_44_TM=59.803
PRIMER_RIGHT_44_TM=60.241
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=720
PRIMER_PAIR_45_PENALTY=0.438078
PRIMER_LEFT_45_PENALTY=0.159749
PRIMER_RIGHT_45_PENALTY=0.278329
PRIMER_LEFT_45_SEQUENCE=CTGCGAAATCACAACACCAC
PRIMER_RIGHT_45_SEQUENCE=ACTGTAATCGCGGCTTTGTC
PRIMER_LEFT_45=599,20
PRIMER_RIGHT_45=1335,20
PRIMER_LEFT_45_TM=60.160
PRIMER_RIGHT_45_TM=60.278
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=737
PRIMER_PAIR_46_PENALTY=0.440278
PRIMER_LEFT_46_PENALTY=0.199246
PRIMER_RIGHT_46_PENALTY=0.241032
PRIMER_LEFT_46_SEQUENCE=CTATCGAGGAAGGCGAAATG
PRIMER_RIGHT_46_SEQUENCE=TTGCCTTACGAGGAGGTTTG
PRIMER_LEFT_46=494,20
PRIMER_RIGHT_46=1254,20
PRIMER_LEFT_46_TM=59.801
PRIMER_RIGHT_46_TM=60.241
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.2000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=761
PRIMER_PAIR_47_PENALTY=0.450087
PRIMER_LEFT_47_PENALTY=0.051345
PRIMER_RIGHT_47_PENALTY=0.398742
PRIMER_LEFT_47_SEQUENCE=TCTCTATCGAGGAAGGCGAA
PRIMER_RIGHT_47_SEQUENCE=GGCTCGATTTTGGGATTTCT
PRIMER_LEFT_47=491,20
PRIMER_RIGHT_47=1298,20
PRIMER_LEFT_47_TM=60.051
PRIMER_RIGHT_47_TM=60.399
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=10.2000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=808
PRIMER_PAIR_48_PENALTY=0.450087
PRIMER_LEFT_48_PENALTY=0.051345
PRIMER_RIGHT_48_PENALTY=0.398742
PRIMER_LEFT_48_SEQUENCE=TTCTCTATCGAGGAAGGCGA
PRIMER_RIGHT_48_SEQUENCE=GGCTCGATTTTGGGATTTCT
PRIMER_LEFT_48=490,20
PRIMER_RIGHT_48=1298,20
PRIMER_LEFT_48_TM=60.051
PRIMER_RIGHT_48_TM=60.399
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=11.4000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=809
PRIMER_PAIR_49_PENALTY=0.461203
PRIMER_LEFT_49_PENALTY=0.196903
PRIMER_RIGHT_49_PENALTY=0.264300
PRIMER_LEFT_49_SEQUENCE=CTCATCCAACTCTTGCTCCC
PRIMER_RIGHT_49_SEQUENCE=ATTTAACCGCTTCTGCGTTG
PRIMER_LEFT_49=535,20
PRIMER_RIGHT_49=1271,20
PRIMER_LEFT_49_TM=59.803
PRIMER_RIGHT_49_TM=60.264
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=8.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=737
=
SEQUENCE_ID=MAGIC.446_5_18272481_18273224_rsch-4_po-0_39591_58844_TRUE
SEQUENCE_TARGET=950,843
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTAANTACTTACAGGGCTACTTTGTCTCCACTCTTGCAGTAGGCTGTTACGATAGCAACNGGTACTNTCTCNGNGTANGCATTATCGATAAANCTANAGCTCAAAAAGGTNAAANNNTTTGAANTTACNNNNNNCAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACANTTTGAAAGANATATCTANGNGACTCACTCTTGNACTCCAGATCTCAGAGNTAANCTTTTCGANATGAGAAACTCATCCATTGCATTTTTCTGACAACAAACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCTTAANTNNAAAGANAATATNGATAGCAGAAAATAACACTATGTTNAAGTANGCANGNAACCTTTTCTCGCAATACACTCTTTACAAAACTTGCTTTCTCACTGGTTGGCAACGANGAAGGCCAACCAATCTGAANNGAGGNNNNNNNNCAAGTGAGNANANAANCTGNCANATTTTGANAACTGTNTGNTCGCANNTCNNTAATGATGTTTANGCNNNNAANNNTGATAANTTACCTGGTTGAAATACAAAAGCAACATTTTCTCTTCATCNGCAGCACCNTTTCTGGTNNNAACNAACTNAACACTNTCAGACAACGAAATGNAATTGTANNATGAACNNNNTCTAANNNNNNNNNNNNNNNNNNNNGNAATCGAANNGGAAANAGCCGTTGATTATGANNGGAAGCANAAANNGTTTGAATGNAACNAAATTATCAANTTAGTATCTANNNACTNNTANAAGANNTNGANCTTTTTNAATCCCTAGNAACAGAATNTAAGAGATGGAAATGGAGATGANTGAAAACAAATAGATTCTTCAAGGTCACAGATAGATGNATTATAAACCNATNGCAACAAAANAATCAATNCTTAAGTATTNNAANGTAAACTTCAATGAACCNNNNNNNNNNNNNAAAACACAAGTCACAAACTTNGATATATATACCTCAACAAGTCTGAATAAACTGGTTCNGGCCAATTTGCACAATCNANCCCAAGCTTTTGAAAAGCTNGTANTCACAATAAGAAGCAACAACATCAGCACAATAAAATAAAAAAACAATCACTGAGCNCAAAAGAATGGGTGNTTTAAAAGCCGCACCTACATTGAACGCTTGGCGATTACTTGACCATGTATCAATCATAACCCTGACATTAAAACAGAGCCAANCAAANAANCAAAAGTTACCNATCACTGAATCNAAAACCAACAACTTGCTTCNAAACTCNGAAAGTTTGAAGCTTTTTCATAATCNGAACTTACTTACCNATCAACCTCAAGTATCACAGCGAATTCTTCAGATGGGTTTACGTCTTCTCTGCTAGCTGATAAGCAAATCGAAGAGAATCTCTGCAATCGCAAGCATTTTCCGAGAATCTGAGATTTTCCAGGGAAATTTGAAAAAGGCAAGAAATTGCGTCTTCCAGTGTCGTNTGAAGANCGCTCGCTCAAATACTGAGTGAAANNAGAAGTCTTTGTACAAGAAATTTGAAGATTGTTAAGAATTGAACAAGAAGCGATTTCCATGGCTCTCTTTTTTCTGCNGGTTCATACACAACACTAGTATACAAANATCGTGTCCAATGATNAAATGATTATTGGGATTAGCATAAAAATAAAGNACACAACTTTACNGGACTATTCCGAATTTCAGACTTTNGGTCCCTNNCACTCGTTTAACTCTNNNNNNNNNNNNNNNNNNNNNNNNATNNNTNTTNNNNNAAAAAATCGGATTTTTATTCTAATNAAATCNTGTTANTCCGCATAATAAANNTTTANTTATGNATCGGATTTNTANTGTTTTCTTGTATTGGAGATTACAGAACAAATTGTAGTATTTGTAGNTTAATGCATNNAAAAGTNTTGTCNTTAACTCATCCTCTTCNGTTTTAGTNTNGTTCTATTGTANATTCAAATTTCTCNGCTTTATGTNGNATTTNNNNNNAAANCGTNNTNTTTTTNNTNAATAAATTTTACATTCATANAAAANAAAANTNCNGAANAACANGANTTNCATAAAANTNTTTGTAGTGTTTTNTTNTTTNGAGNNTGTTCTTTGGANNNNNNNNTANTCTCTGGATAGGTTCGGTGGNNNNNGGAAGAANGTAGNTGAAACTTGAGTANATTGCTGATAGNGGATTTTTAGTGGACTATTGNCNNNNNNATNTTACTCTTCACAATGAAAAGGTTTNNTCTACGTATAATTTGGTGTCACGNNNNNNNNNNNNNNTTAAGCTTTTGCTGCCAACTCACAAACGCANNTAAACGTGGGTAAGATTNNNNNNTGANTATGATGCGCTGTGTTGTGCNTATTTCATAGCATCTTGATGGCANTGAAATACGATTTTGGAANCAACCTCCAATGCACTTCTCATATAACCATNATAGAGNAGGNAAANGGAGAAAANNATTATTTCATTTATTTAAAAGCAAACNATACAANAAACTNAAACATATAAAANCAAATTAGGATGANAAANNGTCNTGTGCATTATTNATCTGAGGATNNAAAGACAATCCNANATCTNTNACNNNNNNNNTTATTTTAATGAAGNNGATNATCNTTTTGAATTACGCTAAGGCTNGAGATTCATGTCNTTAGACAAATCATCNGCATGTTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_16742266_16742517_wil-2_ws-0_752143_282575_TRUE
SEQUENCE_TARGET=950,351
SEQUENCE_TEMPLATE=ATGATTGTAGCACTGCTCTTCATGTTGATTGTTTGCTTGGTGTAGACATGTACATGAAACCTATTACAGACTACATTTCTGTCATCANGCTCACATCTAAGGGTANGAAACGGAAAGATCTTTGGATTTTTCTTAACAANTCTCTCACTCGACCAATTTGCACTACATGCTTAAGTCGTTGTCCATTCCCAATATTTTTCAAAGGGCACACCAAAATCTTCTGTTCTTTGTATTGTAGTGAGGANTGATCATCAAACTAGTTATCTCTTTTCTCTGTCAATCTAGTTATCCCTTNTCTNTGNCAATTTGAAGTATTTTTGTTTTGGGTTTGTTGTANTTCTTAATTGGTGGATATGTNAAAATACAAGATATTTNTGGTTGTGTCTTCTCATCGCATCTTTTTGTTAAAATTTNNCATTTTTNGGTAAANTTTTAGTAATAATTCAAATATTTGTNTATATCATAATAATTTAATACATAAATACTAGGTTCAANGATTCNGTTTNATTCANTTTTTCGGTTCTAGCCTCTTAGGATCCATAAGAGAACCAAAAAAGTNTCGGGTTNAGGTTCGCATACTTAGAAAGAGAGCNGAAAATTAACTNAACTCCATTAATAGATATCCNTTNCGTTTGGTTCCCGTTATTTTACANAAAATACNAAAAAAANNCGAAAAANCCAAANATCNAAAACNTTTTTGACGAAATATCTATGAACAAAAATATTANAATATTGACAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNATGGAANCAAAANTGTTTGGTCNACCCTATACAAAACTACATATAAGCGCAANACATTTTGTTCCAACTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_6016402_6016904_bur-0_hi-0_91460_2273748_TRUE
SEQUENCE_TARGET=950,602
SEQUENCE_TEMPLATE=CCGACTGATTTCAAATCCAAATCTCCCCAGAACCCTAATCCCAAACCTTCCTCATGCCCATTTTGCATCGGTCGTGAGCAAGAGTGTGCCCCGGAGNTATTTCGTGTGCCAGATCATGACCCGAATTGGAAACTCCGGGTCATCGAGAATCTGTACCCGGCTCTTAGTAGGAATCTCGAGACCCAATCGACCCAANCTGAGACGGGAACAAGTCGGACAATAGTCGGATTCGGATTCCACGACGTAGTGATCGAATCCCCTGTTCATTCGATTCAGCTCTCCGATATNGATCCGGTGGGTATCGGCGATNTCTTGATCGCTTATAAGAAGAGGATCAATCAGATTGCGCAACATGATTCCATCAATTACATCCAGGTAAGCATTNTCCATAGATCCCAATGTAGTAGCTNNATTAGTGTGNATTTATACATTGTTTAGCCGTCAATACTTGTGTTATGTGTGGATATTTTGTGTTGCTTGCATAGTTACATTCATCTGCTACGTTTGTTTTCGTATGTTGAAGGTTTTCAAGAACCAAGGTGCGTCGGCTGGAGCATCAATGAGTCACTCCCACAGTCAAATGATGGCTCTACCAGTTGTTCCTCCAACGGTTTCTTCACGCCTTGATGGTACTAAAGATTATTTCGAGGAGACTGGGAAATGTTGTCTCTGTGAAGCTANATCAAAACATTTTGTGATCGATGAGTCGTCTCATTTTGTTTCTGTTGNTCCTTTTGCTGCTACGTATCCCTTTGAGATTTGGATTATTCCGAAAGATCATTCTTCCCACTTCCATCATCTCGNTGACGTTAAGGTTACCTTTTACTGATCTACTATGTTCCATTTATGGTTTGGTTAGATGATCACCTGACTCNCTTTGTTTTGATGATTATCAGGCTGTTGACCTTGGAGGACTTCTGAAACNCATGCTTCAGAAGATANCAAAGCANCTNAANGATCCNCCGTATAACTATATGATCCATACTTCTCCNCTCAAGGTNACAGAATCACAANTACCTTATACGCACTGGTTCTTACAGATTGTACCTCAGCTTTCTGGGGTAGGTGGGTTTGAGATTGGCACAGGTTGTTACATAAACCCTGTTTTCCCAGAGGATGTAGCAAAGGTTATGCGGGAAGTTAGCCTTACTTGAACCCGNTTTTTTAGTCTCAACCTAAAGTTCAGCTGGAAGTGATTAGTGTTGAGTTATTGGCCTNATNTGCAAGTCTCTANAAAACACTATTCAATTGTNTTTTTNTCCTCTGTTGAAGAGGAAGATGTTTCAGCTTGATTCAAATAACACTGATGTNAACAATGCATGTACTGTAGTTCTTTGTAAAACAAGCTCATATCAGGGNATTAAGAAGATTCTTTGATCAATCAGCTTTTAAATTCAGCATCAAACCAATTAACCNNNNNNNNNNNNNNNNNNCGAACCAAACCAGACGATGCTTGANGGCGTCGTTNAAAATGNTTNTAAAGGGNAATTNTNAGAGCNNCTNTCTCAAAAACTCNAANCAGCGAGTGAAAAAAACACAGAGAGCTTCCTCACTTGCTTATAGCTTAGGGTTTACGTTCAAGGCNTAGATCTTCGTCGGAAACAATGGGNATCTCTACGCAGTCTCATGGAATTCAATCGATGCTTAAAGNAGGTTANAGACATCTCGCTGGTCTTGATGAGGCCGTCATCAAAAACANTGAAGCTTGCAAGGAGCTTTCCACCNTTACCCGCACTTCTCTTNGCCCCAATGGTTTGCCTCTCGTCGTCGGATTCGAAATCTATCGTTTCTGTAATGTTAGAATCTGTTTATGATTATTGTTCTGATTTAGGAATGAATAAAGACTGAGCCGATGCTTATATGCAAAGTGTGGCTAACTATGGTGCTTATAAATGGCAGATTATAATGGCTAAACCAGCAGGTGGGCCGAGGAGAGATGCAGCTGCAGCAGCCGGTGCAGGCATGGACGAAGACTAAGCTGTTATAACAATCACCTNTTAGCCTAACANCTCTTCTTCTATATTCATTAGTTTCTTTTTTNATCTTTCTTAGAAAAGTNTTTCTTATAAAAGTTTAATCTTTCTTCTGCCTGTCATTGGTTCCTCTCAACATTTGACANTGAATAGTTGTAATGACAAAGTTTTAGTTGGGTTTTAGACAAAAATGATCAANATTCTAACAAAACAATATAAGAACTAGTTNNATTCTGGCTCTAGTNNTCATTCTGGTTACACAGTAAAATTAAAGATGGAGATAACCTCCCNTAAGTAAAATGTACTAGAATTTTGAGAAGTCCAAAATCTTAAACCCCNAACGGCGTCGNTTNNNAACACTAACGCCGNCGTTTAGCAACACTAACGNCGNCTTGTTCAGTTCCACAGNNNNNNNTCAAAACAGAGTACAGTCACACAGTCAACGAAGATACNNNTAAGTCAATTTTCGTTTGACCAAANTTGATTCTTCATGGCTTCCTCAGTNTCCTCTCTCGCCGGACGAGTCGCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.163344
PRIMER_LEFT_0_PENALTY=0.656393
PRIMER_RIGHT_0_PENALTY=1.506951
PRIMER_LEFT_0_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_0_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_0=891,20
PRIMER_RIGHT_0=1682,20
PRIMER_LEFT_0_TM=60.656
PRIMER_RIGHT_0_TM=61.507
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=792
PRIMER_PAIR_1_PENALTY=2.295925
PRIMER_LEFT_1_PENALTY=0.656393
PRIMER_RIGHT_1_PENALTY=1.639532
PRIMER_LEFT_1_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_1_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_1=891,20
PRIMER_RIGHT_1=1863,21
PRIMER_LEFT_1_TM=60.656
PRIMER_RIGHT_1_TM=59.360
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=2.564671
PRIMER_LEFT_2_PENALTY=1.057719
PRIMER_RIGHT_2_PENALTY=1.506951
PRIMER_LEFT_2_SEQUENCE=TGCTGCTACGTATCCCTTTG
PRIMER_RIGHT_2_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_2=735,20
PRIMER_RIGHT_2=1682,20
PRIMER_LEFT_2_TM=58.942
PRIMER_RIGHT_2_TM=61.507
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=948
PRIMER_PAIR_3_PENALTY=2.577113
PRIMER_LEFT_3_PENALTY=0.187324
PRIMER_RIGHT_3_PENALTY=2.389789
PRIMER_LEFT_3_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_3_SEQUENCE=TTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_3=643,20
PRIMER_RIGHT_3=1631,21
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=58.610
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=989
PRIMER_PAIR_4_PENALTY=2.629518
PRIMER_LEFT_4_PENALTY=0.656393
PRIMER_RIGHT_4_PENALTY=1.973125
PRIMER_LEFT_4_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_4_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_4=891,20
PRIMER_RIGHT_4=1767,20
PRIMER_LEFT_4_TM=60.656
PRIMER_RIGHT_4_TM=61.973
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=877
PRIMER_PAIR_5_PENALTY=2.712413
PRIMER_LEFT_5_PENALTY=0.656393
PRIMER_RIGHT_5_PENALTY=2.056021
PRIMER_LEFT_5_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_5_SEQUENCE=GCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_5=891,20
PRIMER_RIGHT_5=1855,21
PRIMER_LEFT_5_TM=60.656
PRIMER_RIGHT_5_TM=58.944
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=5.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=965
PRIMER_PAIR_6_PENALTY=2.751838
PRIMER_LEFT_6_PENALTY=0.656393
PRIMER_RIGHT_6_PENALTY=2.095445
PRIMER_LEFT_6_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_6_SEQUENCE=CGGCTCAGTCTTTATTCATTCC
PRIMER_LEFT_6=891,20
PRIMER_RIGHT_6=1851,22
PRIMER_LEFT_6_TM=60.656
PRIMER_RIGHT_6_TM=60.095
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=961
PRIMER_PAIR_7_PENALTY=2.772896
PRIMER_LEFT_7_PENALTY=0.656393
PRIMER_RIGHT_7_PENALTY=2.116503
PRIMER_LEFT_7_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_7_SEQUENCE=AGCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_7=891,20
PRIMER_RIGHT_7=1856,22
PRIMER_LEFT_7_TM=60.656
PRIMER_RIGHT_7_TM=59.883
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=5.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=966
PRIMER_PAIR_8_PENALTY=2.777735
PRIMER_LEFT_8_PENALTY=1.270784
PRIMER_RIGHT_8_PENALTY=1.506951
PRIMER_LEFT_8_SEQUENCE=TTGCTGCTACGTATCCCTTTG
PRIMER_RIGHT_8_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_8=734,21
PRIMER_RIGHT_8=1682,20
PRIMER_LEFT_8_TM=60.271
PRIMER_RIGHT_8_TM=61.507
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=949
PRIMER_PAIR_9_PENALTY=2.896891
PRIMER_LEFT_9_PENALTY=1.389940
PRIMER_RIGHT_9_PENALTY=1.506951
PRIMER_LEFT_9_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_9_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_9=892,20
PRIMER_RIGHT_9=1682,20
PRIMER_LEFT_9_TM=61.390
PRIMER_RIGHT_9_TM=61.507
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=791
PRIMER_PAIR_10_PENALTY=2.929675
PRIMER_LEFT_10_PENALTY=0.656393
PRIMER_RIGHT_10_PENALTY=2.273283
PRIMER_LEFT_10_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_10_SEQUENCE=TAAGCATCGGCTCAGTCTTT
PRIMER_LEFT_10=891,20
PRIMER_RIGHT_10=1858,20
PRIMER_LEFT_10_TM=60.656
PRIMER_RIGHT_10_TM=57.727
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=968
PRIMER_PAIR_11_PENALTY=2.952360
PRIMER_LEFT_11_PENALTY=1.445408
PRIMER_RIGHT_11_PENALTY=1.506951
PRIMER_LEFT_11_SEQUENCE=TCATTCTTCCCACTTCCATCA
PRIMER_RIGHT_11_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_11=777,21
PRIMER_RIGHT_11=1682,20
PRIMER_LEFT_11_TM=60.445
PRIMER_RIGHT_11_TM=61.507
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=906
PRIMER_PAIR_12_PENALTY=3.019661
PRIMER_LEFT_12_PENALTY=1.512710
PRIMER_RIGHT_12_PENALTY=1.506951
PRIMER_LEFT_12_SEQUENCE=CATTCTTCCCACTTCCATCA
PRIMER_RIGHT_12_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_12=778,20
PRIMER_RIGHT_12=1682,20
PRIMER_LEFT_12_TM=58.487
PRIMER_RIGHT_12_TM=61.507
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=905
PRIMER_PAIR_13_PENALTY=3.029472
PRIMER_LEFT_13_PENALTY=1.389940
PRIMER_RIGHT_13_PENALTY=1.639532
PRIMER_LEFT_13_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_13_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_13=892,20
PRIMER_RIGHT_13=1863,21
PRIMER_LEFT_13_TM=61.390
PRIMER_RIGHT_13_TM=59.360
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=972
PRIMER_PAIR_14_PENALTY=3.046182
PRIMER_LEFT_14_PENALTY=0.656393
PRIMER_RIGHT_14_PENALTY=2.389789
PRIMER_LEFT_14_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_14_SEQUENCE=TTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_14=891,20
PRIMER_RIGHT_14=1631,21
PRIMER_LEFT_14_TM=60.656
PRIMER_RIGHT_14_TM=58.610
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=741
PRIMER_PAIR_15_PENALTY=3.148695
PRIMER_LEFT_15_PENALTY=1.641744
PRIMER_RIGHT_15_PENALTY=1.506951
PRIMER_LEFT_15_SEQUENCE=TATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_15_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_15=890,21
PRIMER_RIGHT_15=1682,20
PRIMER_LEFT_15_TM=60.642
PRIMER_RIGHT_15_TM=61.507
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=793
PRIMER_PAIR_16_PENALTY=3.153824
PRIMER_LEFT_16_PENALTY=0.187324
PRIMER_RIGHT_16_PENALTY=2.966501
PRIMER_LEFT_16_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_16_SEQUENCE=TCCATGAGACTGCGTAGAGA
PRIMER_LEFT_16=643,20
PRIMER_RIGHT_16=1630,20
PRIMER_LEFT_16_TM=60.187
PRIMER_RIGHT_16_TM=57.033
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=988
PRIMER_PAIR_17_PENALTY=3.154971
PRIMER_LEFT_17_PENALTY=0.088296
PRIMER_RIGHT_17_PENALTY=3.066675
PRIMER_LEFT_17_SEQUENCE=TCCTCCAACGGTTTCTTCAC
PRIMER_RIGHT_17_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_17=600,20
PRIMER_RIGHT_17=1583,20
PRIMER_LEFT_17_TM=60.088
PRIMER_RIGHT_17_TM=56.933
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.5000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=984
PRIMER_PAIR_18_PENALTY=3.182199
PRIMER_LEFT_18_PENALTY=1.675248
PRIMER_RIGHT_18_PENALTY=1.506951
PRIMER_LEFT_18_SEQUENCE=GCTGTTGACCTTGGAGGACTT
PRIMER_RIGHT_18_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_18=896,21
PRIMER_RIGHT_18=1682,20
PRIMER_LEFT_18_TM=60.675
PRIMER_RIGHT_18_TM=61.507
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=787
PRIMER_PAIR_19_PENALTY=3.225990
PRIMER_LEFT_19_PENALTY=0.187324
PRIMER_RIGHT_19_PENALTY=3.038666
PRIMER_LEFT_19_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_19_SEQUENCE=ATTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_19=643,20
PRIMER_RIGHT_19=1632,22
PRIMER_LEFT_19_TM=60.187
PRIMER_RIGHT_19_TM=58.961
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=990
PRIMER_PAIR_20_PENALTY=3.236591
PRIMER_LEFT_20_PENALTY=0.656393
PRIMER_RIGHT_20_PENALTY=2.580198
PRIMER_LEFT_20_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_20_SEQUENCE=AAGCATCGGCTCAGTCTTTATT
PRIMER_LEFT_20=891,20
PRIMER_RIGHT_20=1857,22
PRIMER_LEFT_20_TM=60.656
PRIMER_RIGHT_20_TM=59.420
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=6.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=967
PRIMER_PAIR_21_PENALTY=3.253998
PRIMER_LEFT_21_PENALTY=0.187324
PRIMER_RIGHT_21_PENALTY=3.066675
PRIMER_LEFT_21_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_21_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_21=643,20
PRIMER_RIGHT_21=1583,20
PRIMER_LEFT_21_TM=60.187
PRIMER_RIGHT_21_TM=56.933
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.2000
PRIMER_RIGHT_21_END_STABILITY=7.5000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=941
PRIMER_PAIR_22_PENALTY=3.281276
PRIMER_LEFT_22_PENALTY=1.641744
PRIMER_RIGHT_22_PENALTY=1.639532
PRIMER_LEFT_22_SEQUENCE=TATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_22_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_22=890,21
PRIMER_RIGHT_22=1863,21
PRIMER_LEFT_22_TM=60.642
PRIMER_RIGHT_22_TM=59.360
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=974
PRIMER_PAIR_23_PENALTY=3.283979
PRIMER_LEFT_23_PENALTY=1.777028
PRIMER_RIGHT_23_PENALTY=1.506951
PRIMER_LEFT_23_SEQUENCE=TGCTGCTACGTATCCCTTTGA
PRIMER_RIGHT_23_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_23=735,21
PRIMER_RIGHT_23=1682,20
PRIMER_LEFT_23_TM=60.777
PRIMER_RIGHT_23_TM=61.507
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=948
PRIMER_PAIR_24_PENALTY=3.314780
PRIMER_LEFT_24_PENALTY=1.675248
PRIMER_RIGHT_24_PENALTY=1.639532
PRIMER_LEFT_24_SEQUENCE=GCTGTTGACCTTGGAGGACTT
PRIMER_RIGHT_24_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_24=896,21
PRIMER_RIGHT_24=1863,21
PRIMER_LEFT_24_TM=60.675
PRIMER_RIGHT_24_TM=59.360
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=968
PRIMER_PAIR_25_PENALTY=3.363065
PRIMER_LEFT_25_PENALTY=1.389940
PRIMER_RIGHT_25_PENALTY=1.973125
PRIMER_LEFT_25_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_25_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_25=892,20
PRIMER_RIGHT_25=1767,20
PRIMER_LEFT_25_TM=61.390
PRIMER_RIGHT_25_TM=61.973
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=876
PRIMER_PAIR_26_PENALTY=3.418533
PRIMER_LEFT_26_PENALTY=1.445408
PRIMER_RIGHT_26_PENALTY=1.973125
PRIMER_LEFT_26_SEQUENCE=TCATTCTTCCCACTTCCATCA
PRIMER_RIGHT_26_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_26=777,21
PRIMER_RIGHT_26=1767,20
PRIMER_LEFT_26_TM=60.445
PRIMER_RIGHT_26_TM=61.973
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.9000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=991
PRIMER_PAIR_27_PENALTY=3.445961
PRIMER_LEFT_27_PENALTY=1.389940
PRIMER_RIGHT_27_PENALTY=2.056021
PRIMER_LEFT_27_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_27_SEQUENCE=GCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_27=892,20
PRIMER_RIGHT_27=1855,21
PRIMER_LEFT_27_TM=61.390
PRIMER_RIGHT_27_TM=58.944
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=5.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=964
PRIMER_PAIR_28_PENALTY=3.463313
PRIMER_LEFT_28_PENALTY=0.187324
PRIMER_RIGHT_28_PENALTY=3.275990
PRIMER_LEFT_28_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_28_SEQUENCE=AATTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_28=643,20
PRIMER_RIGHT_28=1633,23
PRIMER_LEFT_28_TM=60.187
PRIMER_RIGHT_28_TM=60.276
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=991
PRIMER_PAIR_29_PENALTY=3.463313
PRIMER_LEFT_29_PENALTY=0.187324
PRIMER_RIGHT_29_PENALTY=3.275990
PRIMER_LEFT_29_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_29_SEQUENCE=GAATTCCATGAGACTGCGTAGAG
PRIMER_LEFT_29=643,20
PRIMER_RIGHT_29=1634,23
PRIMER_LEFT_29_TM=60.187
PRIMER_RIGHT_29_TM=60.276
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.826
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.2000
PRIMER_RIGHT_29_END_STABILITY=5.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=992
PRIMER_PAIR_30_PENALTY=3.477222
PRIMER_LEFT_30_PENALTY=1.970271
PRIMER_RIGHT_30_PENALTY=1.506951
PRIMER_LEFT_30_SEQUENCE=CGTATCCCTTTGAGATTTGGA
PRIMER_RIGHT_30_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_30=743,21
PRIMER_RIGHT_30=1682,20
PRIMER_LEFT_30_TM=59.030
PRIMER_RIGHT_30_TM=61.507
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=940
PRIMER_PAIR_31_PENALTY=3.485385
PRIMER_LEFT_31_PENALTY=1.389940
PRIMER_RIGHT_31_PENALTY=2.095445
PRIMER_LEFT_31_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_31_SEQUENCE=CGGCTCAGTCTTTATTCATTCC
PRIMER_LEFT_31=892,20
PRIMER_RIGHT_31=1851,22
PRIMER_LEFT_31_TM=61.390
PRIMER_RIGHT_31_TM=60.095
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=960
PRIMER_PAIR_32_PENALTY=3.485835
PRIMER_LEFT_32_PENALTY=1.512710
PRIMER_RIGHT_32_PENALTY=1.973125
PRIMER_LEFT_32_SEQUENCE=CATTCTTCCCACTTCCATCA
PRIMER_RIGHT_32_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_32=778,20
PRIMER_RIGHT_32=1767,20
PRIMER_LEFT_32_TM=58.487
PRIMER_RIGHT_32_TM=61.973
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=990
PRIMER_PAIR_33_PENALTY=3.496831
PRIMER_LEFT_33_PENALTY=1.989880
PRIMER_RIGHT_33_PENALTY=1.506951
PRIMER_LEFT_33_SEQUENCE=GCTGCTACGTATCCCTTTGAG
PRIMER_RIGHT_33_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_33=736,21
PRIMER_RIGHT_33=1682,20
PRIMER_LEFT_33_TM=59.010
PRIMER_RIGHT_33_TM=61.507
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=947
PRIMER_PAIR_34_PENALTY=3.498161
PRIMER_LEFT_34_PENALTY=0.431486
PRIMER_RIGHT_34_PENALTY=3.066675
PRIMER_LEFT_34_SEQUENCE=TGTTCCTCCAACGGTTTCTT
PRIMER_RIGHT_34_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_34=597,20
PRIMER_RIGHT_34=1583,20
PRIMER_LEFT_34_TM=59.569
PRIMER_RIGHT_34_TM=56.933
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.5000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=987
PRIMER_PAIR_35_PENALTY=3.498161
PRIMER_LEFT_35_PENALTY=0.431486
PRIMER_RIGHT_35_PENALTY=3.066675
PRIMER_LEFT_35_SEQUENCE=TTGTTCCTCCAACGGTTTCT
PRIMER_RIGHT_35_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_35=596,20
PRIMER_RIGHT_35=1583,20
PRIMER_LEFT_35_TM=59.569
PRIMER_RIGHT_35_TM=56.933
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.5000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=988
PRIMER_PAIR_36_PENALTY=3.506443
PRIMER_LEFT_36_PENALTY=1.389940
PRIMER_RIGHT_36_PENALTY=2.116503
PRIMER_LEFT_36_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_36_SEQUENCE=AGCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_36=892,20
PRIMER_RIGHT_36=1856,22
PRIMER_LEFT_36_TM=61.390
PRIMER_RIGHT_36_TM=59.883
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=5.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=965
PRIMER_PAIR_37_PENALTY=3.508257
PRIMER_LEFT_37_PENALTY=2.001306
PRIMER_RIGHT_37_PENALTY=1.506951
PRIMER_LEFT_37_SEQUENCE=GCTGCTACGTATCCCTTTGA
PRIMER_RIGHT_37_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_37=736,20
PRIMER_RIGHT_37=1682,20
PRIMER_LEFT_37_TM=57.999
PRIMER_RIGHT_37_TM=61.507
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.3000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=947
PRIMER_PAIR_38_PENALTY=3.545884
PRIMER_LEFT_38_PENALTY=0.656393
PRIMER_RIGHT_38_PENALTY=2.889491
PRIMER_LEFT_38_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_38_SEQUENCE=AGCATCGGCTCAGTCTTTATT
PRIMER_LEFT_38=891,20
PRIMER_RIGHT_38=1856,21
PRIMER_LEFT_38_TM=60.656
PRIMER_RIGHT_38_TM=58.111
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=6.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=966
PRIMER_PAIR_39_PENALTY=3.545884
PRIMER_LEFT_39_PENALTY=0.656393
PRIMER_RIGHT_39_PENALTY=2.889491
PRIMER_LEFT_39_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_39_SEQUENCE=ATAAGCATCGGCTCAGTCTTT
PRIMER_LEFT_39=891,20
PRIMER_RIGHT_39=1859,21
PRIMER_LEFT_39_TM=60.656
PRIMER_RIGHT_39_TM=58.111
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=969
PRIMER_PAIR_40_PENALTY=3.566826
PRIMER_LEFT_40_PENALTY=2.059875
PRIMER_RIGHT_40_PENALTY=1.506951
PRIMER_LEFT_40_SEQUENCE=AAGATCATTCTTCCCACTTCCA
PRIMER_RIGHT_40_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_40=773,22
PRIMER_RIGHT_40=1682,20
PRIMER_LEFT_40_TM=59.940
PRIMER_RIGHT_40_TM=61.507
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=910
PRIMER_PAIR_41_PENALTY=3.595427
PRIMER_LEFT_41_PENALTY=2.088476
PRIMER_RIGHT_41_PENALTY=1.506951
PRIMER_LEFT_41_SEQUENCE=TTTGCTGCTACGTATCCCTTT
PRIMER_RIGHT_41_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_41=733,21
PRIMER_RIGHT_41=1682,20
PRIMER_LEFT_41_TM=58.912
PRIMER_RIGHT_41_TM=61.507
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=950
PRIMER_PAIR_42_PENALTY=3.595427
PRIMER_LEFT_42_PENALTY=2.088476
PRIMER_RIGHT_42_PENALTY=1.506951
PRIMER_LEFT_42_SEQUENCE=TTTTGCTGCTACGTATCCCTT
PRIMER_RIGHT_42_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_42=732,21
PRIMER_RIGHT_42=1682,20
PRIMER_LEFT_42_TM=58.912
PRIMER_RIGHT_42_TM=61.507
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=951
PRIMER_PAIR_43_PENALTY=3.598696
PRIMER_LEFT_43_PENALTY=0.656393
PRIMER_RIGHT_43_PENALTY=2.942303
PRIMER_LEFT_43_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_43_SEQUENCE=GCATCGGCTCAGTCTTTATT
PRIMER_LEFT_43=891,20
PRIMER_RIGHT_43=1855,20
PRIMER_LEFT_43_TM=60.656
PRIMER_RIGHT_43_TM=57.058
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=965
PRIMER_PAIR_44_PENALTY=3.614869
PRIMER_LEFT_44_PENALTY=1.641744
PRIMER_RIGHT_44_PENALTY=1.973125
PRIMER_LEFT_44_SEQUENCE=TATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_44_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_44=890,21
PRIMER_RIGHT_44=1767,20
PRIMER_LEFT_44_TM=60.642
PRIMER_RIGHT_44_TM=61.973
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=878
PRIMER_PAIR_45_PENALTY=3.622894
PRIMER_LEFT_45_PENALTY=0.656393
PRIMER_RIGHT_45_PENALTY=2.966501
PRIMER_LEFT_45_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_45_SEQUENCE=TCCATGAGACTGCGTAGAGA
PRIMER_LEFT_45=891,20
PRIMER_RIGHT_45=1630,20
PRIMER_LEFT_45_TM=60.656
PRIMER_RIGHT_45_TM=57.033
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=740
PRIMER_PAIR_46_PENALTY=3.634033
PRIMER_LEFT_46_PENALTY=0.187324
PRIMER_RIGHT_46_PENALTY=3.446709
PRIMER_LEFT_46_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_46_SEQUENCE=TTCCATGAGACTGCGTAGAG
PRIMER_LEFT_46=643,20
PRIMER_RIGHT_46=1631,20
PRIMER_LEFT_46_TM=60.187
PRIMER_RIGHT_46_TM=56.553
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.2000
PRIMER_RIGHT_46_END_STABILITY=5.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=989
PRIMER_PAIR_47_PENALTY=3.637175
PRIMER_LEFT_47_PENALTY=0.570500
PRIMER_RIGHT_47_PENALTY=3.066675
PRIMER_LEFT_47_SEQUENCE=GTTGTTCCTCCAACGGTTTC
PRIMER_RIGHT_47_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_47=595,20
PRIMER_RIGHT_47=1583,20
PRIMER_LEFT_47_TM=59.430
PRIMER_RIGHT_47_TM=56.933
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.5000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=989
PRIMER_PAIR_48_PENALTY=3.648373
PRIMER_LEFT_48_PENALTY=1.675248
PRIMER_RIGHT_48_PENALTY=1.973125
PRIMER_LEFT_48_SEQUENCE=GCTGTTGACCTTGGAGGACTT
PRIMER_RIGHT_48_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_48=896,21
PRIMER_RIGHT_48=1767,20
PRIMER_LEFT_48_TM=60.675
PRIMER_RIGHT_48_TM=61.973
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=872
PRIMER_PAIR_49_PENALTY=3.651899
PRIMER_LEFT_49_PENALTY=2.144948
PRIMER_RIGHT_49_PENALTY=1.506951
PRIMER_LEFT_49_SEQUENCE=AGGCTGTTGACCTTGGAGGA
PRIMER_RIGHT_49_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_49=894,20
PRIMER_RIGHT_49=1682,20
PRIMER_LEFT_49_TM=62.145
PRIMER_RIGHT_49_TM=61.507
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=789
=
SEQUENCE_ID=MAGIC.338_3_114112_114841_oy-0_po-0_114683_854098_TRUE
SEQUENCE_TARGET=950,829
SEQUENCE_TEMPLATE=GGAAGAAGAAGAAAAAGAAGACTGAACAGTTATGGCAACGTAACAAGGTGAGAGTAGAAAGGTTCCATAGGTTTCTCTCAGCTTAAAGATGACCACAACAAAGTTTCCTCTTGGGTTTTCTTCTTTCCTTCAAACCAACAACACACAAGAAAAAGGTAAAACTGNCAAAGTCGCACAANTAAAGTTCTCTGCNACAATTCCCAACTTTGGTCTTCAAGTTTCTTACTATGCTGATTATAAGATGAGATATAGAGAGATGCACAAAGGANCAAAACCATAAGAAGAACAATGGTGGTTTAGAGTTACTGTGTGTATCTTTAGGTTTCAAGTGNNAAGTCTGTTAGTGTTACATGTAGTGTGTGTCTGTGCTCTTTAGGAGAATGTTGTTGTAAATATGGGAAAGCACTTGAAGATGCCTTGGTTAATAGATAGATAGGCCCAAAAGAAAAATTATTATTATATGCTTCAAATTTCAAGATTATGAAATCAACTAAATTTTTTATTGGTNACTAAGCGGTAACAACAACATTTTTGTTTTGGTGAATTAAGAACAAGAAAACAATGAGACATACAGAGACAGAGAAATCATATTTCGTGTTAANTAAGTTTACGAAAATGACTAAACCTAACCCTAATTAGGTTTCATCTTTCCTAAAGAGTAAAAGACCAAAGAATATGTGTNGAAAACCCCTGCATCCANTATATGGATGTTTCATACGTTCAACAATGATGATTTTTTNATCATCTCATAATCGATGGAATAATACTACTTAGTTGTTTTCAACGCATTGAAACACTCCTTAGTGAGGCCGTCGTGATTTGTCTTTAAGACAAGACTTCAAACAAACGGGATGCCTATGAAACATTCAACGAGACANAAATGATTGTTTGCGATTTGTGAAATGTTAAGATTCATTTTTGCTTACGGACTAACTAACCANTGGATGCTTAGGCGCGGTTAGTTTTCAACNTATTTAAAATTTACCANTTTTCCATAAATNAGTATATGTAACTTCCAAATNTTGTATACGTGGCCTTTCTTTACTCACTCTCTTCTTATAGAANGAAATCCTTAGAATACATTNCACGCAGAAGCAATCTCACGTCAATTCTGTACAACTACACAAACTCTATAAACCATNCATCTTCCGTCTTCTGTTGNAAAAAAAAAGCAGAAAATGAAATCAAAGCAAATCAAAATGATGTTNTTTTTATTAANTGTAGTTTCAGCTTTGATTTTGCGACCGTNCAAAGCTCAGATAAAAACGAGCNTATGTTCAAGAACACAGACAAGGCCGATAGATAACGTGGATGGATGTTTCGATGCGGTGAGATTAGCNGCGGATGTAGATATCANATGGCTATCAAGAGACTGTTGCAAAGCAGTGAAAACACTTGATGATTGCCTTTTGCTTGTATTTCCCAACAGAGCTTACAATACTAATCTCTTCAAAGAAATTTGTTTCGAAATTTTCAATAACTNAATTTTATGATTTCTCTGTATTTTATAATGATATAATCAACGAATCAAATTTANTTTTTTAATTCTGATACGAAAATATTAACATTTCACAAATCGCAAACAATCCTATTATATATCTATACATGTCTCGTTCTTTGTTTCATGCTCCTCCNTTTGTTTGAAGTCTTATCACTAAAGAATGCTTCAATGCTTTGTAAACAACCNAGTATTTAGAGATTCCATCGATNANGAGATAACAAATAATCCNNATTGTTGAATGTATGAACATCCATATCGGATGCACGGGTAATCNACANTATTCTTTGGTCAAAGTGAAACCTAATTAGGGTNAGGTTNNNTAGTGATTTTNTTTNAGTTGTTATTTCCAACNTAACTAATCCGAAATATGATCTCGCTGTCTCTAATTATTTTGNTGGTCTTAAGTCACCAAAAATGTTGTTACTGCTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCAAAGATNATCTCTTTTTAANAAAANNAAATNAAAAAGTCCATTGGTCANNNNNNNNNAAGCATATAAAATTACTTTTCCNNNNNNNNNNNNNNNNNNGGGTCAAANCTTGTATATTTAATTAANTACGTCTTTCTTTCTTTACTCNATCTTCTTATAGAAGGANATCCTGANAAANANANNCACAAAAAAAAAANGCATCACGNNNNTTNTCTACANCTACACAAACTCTATAAACCNNNNNNNNNTTTTTCGTCTTCTATTGTAAAACAAAAACAGANAAAATGAAAACAAAGCAAATCANAATGATGTTCTTCGTACTAATNGTAGTTTNAGCTTTGATTTTCCGACCATCCGAAGCTCAGATGAAGATCCAATCGTGGATATGTTCAAGTGAACAGATAGCACCGATAGTAAACGTGAGTGGATGTTTCGATGCGTTGAAATTAGCGTTAGGGTCGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_12766290_12766538_wil-2_no-0_51062_19907_TRUE
SEQUENCE_TARGET=950,348
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAGGATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17367896_17367996_tsu-0_po-0_11918_4803_TRUE
SEQUENCE_TARGET=950,200
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_14381109_14381192_tsu-0_bur-0_51355_16810_TRUE
SEQUENCE_TARGET=950,183
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_8108997_8109297_mt-0_ct-1_164195_1609_TRUE
SEQUENCE_TARGET=950,400
SEQUENCE_TEMPLATE=GATACCATTTTACAGTTCATTCAAAAGTCCCCGGATGATATCGTNTGCAAGATCACTATGACCTGGGAGAAGCGANCCGATGACTCGCCTGAACCCAGCAACTACATGAAGTTGGTCAAGAGTCTCGCTGCTGACATGGACGCCCACGTTCTCAAAGCCTAATCCCTCTCACCTAAACCCATCATCATCGTGTGTGTAACTTTGTATGATGTTTTCAATATAATAAATGGGGGTTTNCATGTATGGTTTCATCTACTNTAATATGCTTTGATGTTGTTTANAATAATTAATGATTTANAGNGTAANTAATCAATATTGTCCTAANTAGTGTTTCACTATATATTGTACTTAGTGGTTGTATTATCCATCATTTTATATGTTCATTTAATGGTTGTATTATTGCTCTCTTTNTTCGTTGACATCTNNNNNTTNTATTNTATTTTATATAATNTTTGTATTCTTTGCACATCNAGTGATNCCNTCANNNNNNATTCCCTGGNGCAACTACTATNTAGGACTACATGCCTATCNACCGGNTACCGCTNCAATACCATATTACCATCTACNAAGTTATCTCTCAGGTTCTGGTTTCANNNNAGGCTNNNNNNNNNNNNNNNNNNTGGTTGTATTATATTCATCAATCATTNTTTTTCTAACTGCATGTTCTTTCTTCTTGAGTACTGTNTCATTATTAATTGGCCNTATATACANAAATAAATATTAGTATTTTTGNTTGGTTTTTCATTTTAAAAATATCATTAAAATTCTAAGATCATATTTNGAAAANNAAAAAANAAAAAGATCGTGATTATTAGAGATTCAAGATAGTTTTCATCGATTTATATTATNGGGTTCAGCCTATCAACATAATCNCATTTAATTNGGAATGAATCCNNNNNACGTNAGGATAGANAGATTGAAGTTGACTAACANAAAACGGAAAGAGTGACTCGAATTTACGTCCAATATTACATAATATTGAGANGGAAAANANAAANGANGAAGATCTTGGATNCGAGAATNTGGAAGAAAATCATATTCGAAGACNTAATAGAAGAANATAAATNGAACAGTCACAAAGCNANGGANNNNNNANTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNCTAACTTGCTATNAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTCTATTGANAAGGCNAGTTGTTGTTGCTTGNATANCTTTCATTCNGGATACCANTNGNGCNATGCCNNTTNGGNNTNNNNNTCTCCCCAANNNNNNNNNNNNNNNNNAGTTGTTGTTGCTTGNATAGCTTTCATTCTGGATACCANTNGTGCNATGCCTGCCCTNNNATNCANANNNNNNNNNNNTTGTGAAAAAGACCATTTCTNCCNCAAACTNAACNACNCATTNCTTTATCTGANTAAGGAAAATGTTCATCCNATCATGATCATTTGACTGCGTAGAGGTCTCNGAATCATCGAGACAAAACCCGCTTNCATCATCTTNNGCCANCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_18316734_18317070_tsu-0_oy-0_506749_75103_TRUE
SEQUENCE_TARGET=950,436
SEQUENCE_TEMPLATE=TTTCTTTCCATGTTGATTTAGCCCATTCGTAGACTTCCTCCGCGCCGCCGTTAGACGCCGCCTCGGAAGTATCAGCAACCGCNGTGGTGTTCGTCGCCTTCAAAGNCTCGTTTCTCTCCTCTCTCTGGTANNNNTTTTNTTATNNNTGGGTTTACTNGTGAAGATTCGGTTATACTTCGGAGATCATTGTTATAATTCACTTGTTCTGATTTAGTCCTACTTGTCAAATTTCTGGATTGTCGAAATTGGCAATTCATTGTCTCAGATCGTANNATTATTGGATCTGATTTTGAATTTTAGAGTTAGAGAGTTCATGTTGGAGTTTTGGGATTAAAATCGTTTCTATNTTCTTCTGGATCTAAAGCATTTTTAGATCGTGGAATTGTGAAACAGCTAATANNNNTTTNTTTNTTTTTTTGCGTTTTGATGATTTTCAATTGCGATTGCANAGGNTTGATCATAAGATCTANACTTGATTCTACTCACTTTATTTGTTGTTTCCTCTAANTTGNAAAATTGAAATCTGCAGAGATCTGAATTTGAGATGTTTAAAAAAATGAGTGCTACTACAAGCAAGACGGATCAGAAAGCGGCATTAGACATTGCATCATGGCTATTCAATGTGNTTACTTCCGTTGGAATCATCCTCGTTAACAAAGCTTTAATGGCTACTTATGGCTTTAGTTTCGGTGAGTTTTTCTTCTTTTATCACAAACTCTNNNNNNNNNNNNTTAGTCAACGGGGTTGACTTGTTTCAANNCACTNCTNAAACTGATTTAAGATCTGTTTCTTAATNTGATACAGCTACTACATTAACCGGTTTGCATTTCGGAACAACGACATTGTTGACNACTTTCTTGACATGGCTTGGTTATATCCAACCTTCTCAGCTTCCTTGGCCTGATCTTTTGAAATTCGTTTTGTTCGCTAACTTCTCTATCGTTGGGATGAACGTCAGTCTCATGTGGAATTCTGTTGGCTTCTACCAGGTCNGNNNNNNNTCATCNCGATGCGGANTTACGTTTTCCTTNGTTTAAAGTTTTTTTTAGTAAGATCTTGAAACGAGTGTGTTGGCATTNNAGATTGCGAAGCTGAGTATGATCCCGGTTTCTTGTCTTCTAGAAGTTATGCTTGACAATGTNAGGTATTCAAGAGATACAAAGCTTAGCATTTTGTTAGTTCTTGCTGGTGTTGCCGTCTGTACTGTAACCGATGTTAGTGTCAATCTCAACGGATTTCTNGCTGCCGCTATTGCTGTCTGGAGCACCGCGCTTCAGCAATACGTAAGTAGTGCCTGAAACGTTAATCTATTGTCAAATTTGGTCAGNTCTTGGTCNAATTCATCCTGATATCGTGATCCTGATCCCACACATTCATTNTATATCATCTAACTTTACTTGTAAACCGAAAAGGAAAATCTGTTTTCTAACAATTGTAACTACACCCTGCTGCAGTATGTACATTATCTACAACGGAAATATNCACTCGGTTCTTTCAATTTACTGGCGCATACCGCTCCAGTTCAAGCTGCGTCATTGTTGTTGGTCGGACCATTTCTAGACTACTGGTTGACTAACCAAAGAGTGGATGCATACAACTTTTCATTTGTATCTCTGGTAAAGTTTCTNCTCNNTTTCTTTACAAAGTCTCCTANNNNNCAAAACNNNNNNNNNAGNATTTGGAACATAGTGTAGTATTAGTAGTCATANNNNNNNGCATTTATTCCATCTCAAGAACTGATTGTTATACTCTGTTGGATTTGTCTTTCCTAAGCTCTATCTAACACANTTNTTTGGGCAGTTCTTCTTAATACTCTCGTGCAGTATCGCGGTNGGGACCAATCTCAGCCAATTCATCTGTATTGGAAGATTCACGGCGGTGTCTTTCCAAGTACTTGGTCACATGAAGACAATTCTGGTTTTGGTTTTGGGATTCACTTTCTTCGGTAAGGAAGGATTAAACCTACAAGTAGTGCTTGGAATGCTGATTGCTATACTCGGTATGATCTGGTACGGTAATGCATCTTCTAAACCGGGTGGTAAAGAACGTCGTAGCCTGTCTATTCCCATAACCAAGTCACAGAAACTATCTGAAACAAACGAATCTGATGAAAAGGTGTAGAAGAAAGAAGTGAATCGCATTGACATAGAAACAAAAACAAGATACTCAGATACAAACAGGTAGAGTTATCAAATGGGATATTATTTATAAANNAAAAAAAAGAAAGGGATTTAATTATTTAATTTCAAGAACTATAATTTTCTTTTCTTACAGAGCTTTTTGTTTTTATGAATCTTACATATTGGTAAATCCTTTTTTGGCCTTTTGCATTTTGC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.343693
PRIMER_LEFT_0_PENALTY=0.183944
PRIMER_RIGHT_0_PENALTY=0.159749
PRIMER_LEFT_0_SEQUENCE=TTCCTTGGCCTGATCTTTTG
PRIMER_RIGHT_0_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_0=891,20
PRIMER_RIGHT_0=1546,20
PRIMER_LEFT_0_TM=60.184
PRIMER_RIGHT_0_TM=60.160
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=10.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=656
PRIMER_PAIR_1_PENALTY=0.352881
PRIMER_LEFT_1_PENALTY=0.193133
PRIMER_RIGHT_1_PENALTY=0.159749
PRIMER_LEFT_1_SEQUENCE=GGATCAGAAAGCGGCATTAG
PRIMER_RIGHT_1_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_1=579,20
PRIMER_RIGHT_1=1546,20
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=60.160
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=968
PRIMER_PAIR_2_PENALTY=0.508382
PRIMER_LEFT_2_PENALTY=0.348633
PRIMER_RIGHT_2_PENALTY=0.159749
PRIMER_LEFT_2_SEQUENCE=AGCTTCCTTGGCCTGATCTT
PRIMER_RIGHT_2_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_2=888,20
PRIMER_RIGHT_2=1546,20
PRIMER_LEFT_2_TM=60.349
PRIMER_RIGHT_2_TM=60.160
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=659
PRIMER_PAIR_3_PENALTY=0.512002
PRIMER_LEFT_3_PENALTY=0.352253
PRIMER_RIGHT_3_PENALTY=0.159749
PRIMER_LEFT_3_SEQUENCE=GGTTTGCATTTCGGAACAAC
PRIMER_RIGHT_3_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_3=818,20
PRIMER_RIGHT_3=1546,20
PRIMER_LEFT_3_TM=60.352
PRIMER_RIGHT_3_TM=60.160
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=729
PRIMER_PAIR_4_PENALTY=0.547989
PRIMER_LEFT_4_PENALTY=0.388240
PRIMER_RIGHT_4_PENALTY=0.159749
PRIMER_LEFT_4_SEQUENCE=AACGGGGTTGACTTGTTTCA
PRIMER_RIGHT_4_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_4=737,20
PRIMER_RIGHT_4=1546,20
PRIMER_LEFT_4_TM=60.388
PRIMER_RIGHT_4_TM=60.160
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=810
PRIMER_PAIR_5_PENALTY=0.547989
PRIMER_LEFT_5_PENALTY=0.388240
PRIMER_RIGHT_5_PENALTY=0.159749
PRIMER_LEFT_5_SEQUENCE=CAACGGGGTTGACTTGTTTC
PRIMER_RIGHT_5_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_5=736,20
PRIMER_RIGHT_5=1546,20
PRIMER_LEFT_5_TM=60.388
PRIMER_RIGHT_5_TM=60.160
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=10.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=811
PRIMER_PAIR_6_PENALTY=0.593361
PRIMER_LEFT_6_PENALTY=0.433612
PRIMER_RIGHT_6_PENALTY=0.159749
PRIMER_LEFT_6_SEQUENCE=TCAGAAAGCGGCATTAGACA
PRIMER_RIGHT_6_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_6=582,20
PRIMER_RIGHT_6=1546,20
PRIMER_LEFT_6_TM=59.566
PRIMER_RIGHT_6_TM=60.160
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=10.2000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=965
PRIMER_PAIR_7_PENALTY=0.652857
PRIMER_LEFT_7_PENALTY=0.183944
PRIMER_RIGHT_7_PENALTY=0.468913
PRIMER_LEFT_7_SEQUENCE=TTCCTTGGCCTGATCTTTTG
PRIMER_RIGHT_7_SEQUENCE=CGCCGTGAATCTTCCAATAC
PRIMER_LEFT_7=891,20
PRIMER_RIGHT_7=1877,20
PRIMER_LEFT_7_TM=60.184
PRIMER_RIGHT_7_TM=60.469
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=5.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=987
PRIMER_PAIR_8_PENALTY=0.676424
PRIMER_LEFT_8_PENALTY=0.516675
PRIMER_RIGHT_8_PENALTY=0.159749
PRIMER_LEFT_8_SEQUENCE=CTTCCGTTGGAATCATCCTC
PRIMER_RIGHT_8_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_8=629,20
PRIMER_RIGHT_8=1546,20
PRIMER_LEFT_8_TM=59.483
PRIMER_RIGHT_8_TM=60.160
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=10.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=918
PRIMER_PAIR_9_PENALTY=0.740454
PRIMER_LEFT_9_PENALTY=0.183944
PRIMER_RIGHT_9_PENALTY=0.556510
PRIMER_LEFT_9_SEQUENCE=TTCCTTGGCCTGATCTTTTG
PRIMER_RIGHT_9_SEQUENCE=TGCGCCAGTAAATTGAAAGA
PRIMER_LEFT_9=891,20
PRIMER_RIGHT_9=1509,20
PRIMER_LEFT_9_TM=60.184
PRIMER_RIGHT_9_TM=59.443
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=619
PRIMER_PAIR_10_PENALTY=0.749643
PRIMER_LEFT_10_PENALTY=0.193133
PRIMER_RIGHT_10_PENALTY=0.556510
PRIMER_LEFT_10_SEQUENCE=GGATCAGAAAGCGGCATTAG
PRIMER_RIGHT_10_SEQUENCE=TGCGCCAGTAAATTGAAAGA
PRIMER_LEFT_10=579,20
PRIMER_RIGHT_10=1509,20
PRIMER_LEFT_10_TM=59.807
PRIMER_RIGHT_10_TM=59.443
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=931
PRIMER_PAIR_11_PENALTY=0.817546
PRIMER_LEFT_11_PENALTY=0.348633
PRIMER_RIGHT_11_PENALTY=0.468913
PRIMER_LEFT_11_SEQUENCE=AGCTTCCTTGGCCTGATCTT
PRIMER_RIGHT_11_SEQUENCE=CGCCGTGAATCTTCCAATAC
PRIMER_LEFT_11=888,20
PRIMER_RIGHT_11=1877,20
PRIMER_LEFT_11_TM=60.349
PRIMER_RIGHT_11_TM=60.469
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=5.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=990
PRIMER_PAIR_12_PENALTY=0.872503
PRIMER_LEFT_12_PENALTY=0.712754
PRIMER_RIGHT_12_PENALTY=0.159749
PRIMER_LEFT_12_SEQUENCE=GCTTCCTTGGCCTGATCTTT
PRIMER_RIGHT_12_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_12=889,20
PRIMER_RIGHT_12=1546,20
PRIMER_LEFT_12_TM=60.713
PRIMER_RIGHT_12_TM=60.160
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=10.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=658
PRIMER_PAIR_13_PENALTY=0.872976
PRIMER_LEFT_13_PENALTY=0.713227
PRIMER_RIGHT_13_PENALTY=0.159749
PRIMER_LEFT_13_SEQUENCE=TCCTTGGCCTGATCTTTTGA
PRIMER_RIGHT_13_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_13=892,20
PRIMER_RIGHT_13=1546,20
PRIMER_LEFT_13_TM=60.713
PRIMER_RIGHT_13_TM=60.160
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=10.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=655
PRIMER_PAIR_14_PENALTY=0.980280
PRIMER_LEFT_14_PENALTY=0.183944
PRIMER_RIGHT_14_PENALTY=0.796337
PRIMER_LEFT_14_SEQUENCE=TTCCTTGGCCTGATCTTTTG
PRIMER_RIGHT_14_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_14=891,20
PRIMER_RIGHT_14=1527,20
PRIMER_LEFT_14_TM=60.184
PRIMER_RIGHT_14_TM=60.796
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=5.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=637
PRIMER_PAIR_15_PENALTY=0.989469
PRIMER_LEFT_15_PENALTY=0.193133
PRIMER_RIGHT_15_PENALTY=0.796337
PRIMER_LEFT_15_SEQUENCE=GGATCAGAAAGCGGCATTAG
PRIMER_RIGHT_15_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_15=579,20
PRIMER_RIGHT_15=1527,20
PRIMER_LEFT_15_TM=59.807
PRIMER_RIGHT_15_TM=60.796
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=5.9000
PRIMER_RIGHT_15_END_STABILITY=5.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=949
PRIMER_PAIR_16_PENALTY=0.990122
PRIMER_LEFT_16_PENALTY=0.433612
PRIMER_RIGHT_16_PENALTY=0.556510
PRIMER_LEFT_16_SEQUENCE=TCAGAAAGCGGCATTAGACA
PRIMER_RIGHT_16_SEQUENCE=TGCGCCAGTAAATTGAAAGA
PRIMER_LEFT_16=582,20
PRIMER_RIGHT_16=1509,20
PRIMER_LEFT_16_TM=59.566
PRIMER_RIGHT_16_TM=59.443
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=928
PRIMER_PAIR_17_PENALTY=1.073186
PRIMER_LEFT_17_PENALTY=0.516675
PRIMER_RIGHT_17_PENALTY=0.556510
PRIMER_LEFT_17_SEQUENCE=CTTCCGTTGGAATCATCCTC
PRIMER_RIGHT_17_SEQUENCE=TGCGCCAGTAAATTGAAAGA
PRIMER_LEFT_17=629,20
PRIMER_RIGHT_17=1509,20
PRIMER_LEFT_17_TM=59.483
PRIMER_RIGHT_17_TM=59.443
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=881
PRIMER_PAIR_18_PENALTY=1.144969
PRIMER_LEFT_18_PENALTY=0.348633
PRIMER_RIGHT_18_PENALTY=0.796337
PRIMER_LEFT_18_SEQUENCE=AGCTTCCTTGGCCTGATCTT
PRIMER_RIGHT_18_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_18=888,20
PRIMER_RIGHT_18=1527,20
PRIMER_LEFT_18_TM=60.349
PRIMER_RIGHT_18_TM=60.796
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=5.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=640
PRIMER_PAIR_19_PENALTY=1.145293
PRIMER_LEFT_19_PENALTY=0.985544
PRIMER_RIGHT_19_PENALTY=0.159749
PRIMER_LEFT_19_SEQUENCE=TCCAACCTTCTCAGCTTCCT
PRIMER_RIGHT_19_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_19=876,20
PRIMER_RIGHT_19=1546,20
PRIMER_LEFT_19_TM=59.014
PRIMER_RIGHT_19_TM=60.160
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=10.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=671
PRIMER_PAIR_20_PENALTY=1.148590
PRIMER_LEFT_20_PENALTY=0.352253
PRIMER_RIGHT_20_PENALTY=0.796337
PRIMER_LEFT_20_SEQUENCE=GGTTTGCATTTCGGAACAAC
PRIMER_RIGHT_20_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_20=818,20
PRIMER_RIGHT_20=1527,20
PRIMER_LEFT_20_TM=60.352
PRIMER_RIGHT_20_TM=60.796
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=5.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=710
PRIMER_PAIR_21_PENALTY=1.160429
PRIMER_LEFT_21_PENALTY=1.000680
PRIMER_RIGHT_21_PENALTY=0.159749
PRIMER_LEFT_21_SEQUENCE=CAAGCAAGACGGATCAGAAAG
PRIMER_RIGHT_21_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_21=569,21
PRIMER_RIGHT_21=1546,20
PRIMER_LEFT_21_TM=60.001
PRIMER_RIGHT_21_TM=60.160
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=10.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=978
PRIMER_PAIR_22_PENALTY=1.161180
PRIMER_LEFT_22_PENALTY=1.001431
PRIMER_RIGHT_22_PENALTY=0.159749
PRIMER_LEFT_22_SEQUENCE=CAAGCAAGACGGATCAGAAA
PRIMER_RIGHT_22_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_22=569,20
PRIMER_RIGHT_22=1546,20
PRIMER_LEFT_22_TM=58.999
PRIMER_RIGHT_22_TM=60.160
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=10.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=978
PRIMER_PAIR_23_PENALTY=1.181667
PRIMER_LEFT_23_PENALTY=0.712754
PRIMER_RIGHT_23_PENALTY=0.468913
PRIMER_LEFT_23_SEQUENCE=GCTTCCTTGGCCTGATCTTT
PRIMER_RIGHT_23_SEQUENCE=CGCCGTGAATCTTCCAATAC
PRIMER_LEFT_23=889,20
PRIMER_RIGHT_23=1877,20
PRIMER_LEFT_23_TM=60.713
PRIMER_RIGHT_23_TM=60.469
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=5.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=989
PRIMER_PAIR_24_PENALTY=1.182140
PRIMER_LEFT_24_PENALTY=0.713227
PRIMER_RIGHT_24_PENALTY=0.468913
PRIMER_LEFT_24_SEQUENCE=TCCTTGGCCTGATCTTTTGA
PRIMER_RIGHT_24_SEQUENCE=CGCCGTGAATCTTCCAATAC
PRIMER_LEFT_24=892,20
PRIMER_RIGHT_24=1877,20
PRIMER_LEFT_24_TM=60.713
PRIMER_RIGHT_24_TM=60.469
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.3000
PRIMER_RIGHT_24_END_STABILITY=5.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=986
PRIMER_PAIR_25_PENALTY=1.184576
PRIMER_LEFT_25_PENALTY=0.388240
PRIMER_RIGHT_25_PENALTY=0.796337
PRIMER_LEFT_25_SEQUENCE=AACGGGGTTGACTTGTTTCA
PRIMER_RIGHT_25_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_25=737,20
PRIMER_RIGHT_25=1527,20
PRIMER_LEFT_25_TM=60.388
PRIMER_RIGHT_25_TM=60.796
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=5.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=791
PRIMER_PAIR_26_PENALTY=1.184576
PRIMER_LEFT_26_PENALTY=0.388240
PRIMER_RIGHT_26_PENALTY=0.796337
PRIMER_LEFT_26_SEQUENCE=CAACGGGGTTGACTTGTTTC
PRIMER_RIGHT_26_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_26=736,20
PRIMER_RIGHT_26=1527,20
PRIMER_LEFT_26_TM=60.388
PRIMER_RIGHT_26_TM=60.796
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=5.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=792
PRIMER_PAIR_27_PENALTY=1.229949
PRIMER_LEFT_27_PENALTY=0.433612
PRIMER_RIGHT_27_PENALTY=0.796337
PRIMER_LEFT_27_SEQUENCE=TCAGAAAGCGGCATTAGACA
PRIMER_RIGHT_27_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_27=582,20
PRIMER_RIGHT_27=1527,20
PRIMER_LEFT_27_TM=59.566
PRIMER_RIGHT_27_TM=60.796
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=5.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=946
PRIMER_PAIR_28_PENALTY=1.254994
PRIMER_LEFT_28_PENALTY=0.183944
PRIMER_RIGHT_28_PENALTY=1.071050
PRIMER_LEFT_28_SEQUENCE=TTCCTTGGCCTGATCTTTTG
PRIMER_RIGHT_28_SEQUENCE=AACAATGACGCAGCTTGAAC
PRIMER_LEFT_28=891,20
PRIMER_RIGHT_28=1539,20
PRIMER_LEFT_28_TM=60.184
PRIMER_RIGHT_28_TM=58.929
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=649
PRIMER_PAIR_29_PENALTY=1.292467
PRIMER_LEFT_29_PENALTY=1.132718
PRIMER_RIGHT_29_PENALTY=0.159749
PRIMER_LEFT_29_SEQUENCE=ACAAGCAAGACGGATCAGAAA
PRIMER_RIGHT_29_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_29=568,21
PRIMER_RIGHT_29=1546,20
PRIMER_LEFT_29_TM=59.867
PRIMER_RIGHT_29_TM=60.160
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=10.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=979
PRIMER_PAIR_30_PENALTY=1.300466
PRIMER_LEFT_30_PENALTY=1.140717
PRIMER_RIGHT_30_PENALTY=0.159749
PRIMER_LEFT_30_SEQUENCE=ATCAGAAAGCGGCATTAGACA
PRIMER_RIGHT_30_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_30=581,21
PRIMER_RIGHT_30=1546,20
PRIMER_LEFT_30_TM=59.859
PRIMER_RIGHT_30_TM=60.160
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=10.2000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=966
PRIMER_PAIR_31_PENALTY=1.313012
PRIMER_LEFT_31_PENALTY=0.516675
PRIMER_RIGHT_31_PENALTY=0.796337
PRIMER_LEFT_31_SEQUENCE=CTTCCGTTGGAATCATCCTC
PRIMER_RIGHT_31_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_31=629,20
PRIMER_RIGHT_31=1527,20
PRIMER_LEFT_31_TM=59.483
PRIMER_RIGHT_31_TM=60.796
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=5.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=899
PRIMER_PAIR_32_PENALTY=1.354219
PRIMER_LEFT_32_PENALTY=0.183944
PRIMER_RIGHT_32_PENALTY=1.170275
PRIMER_LEFT_32_SEQUENCE=TTCCTTGGCCTGATCTTTTG
PRIMER_RIGHT_32_SEQUENCE=GTCCGACCAACAACAATGAC
PRIMER_LEFT_32=891,20
PRIMER_RIGHT_32=1550,20
PRIMER_LEFT_32_TM=60.184
PRIMER_RIGHT_32_TM=58.830
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=6.3000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=660
PRIMER_PAIR_33_PENALTY=1.363408
PRIMER_LEFT_33_PENALTY=0.193133
PRIMER_RIGHT_33_PENALTY=1.170275
PRIMER_LEFT_33_SEQUENCE=GGATCAGAAAGCGGCATTAG
PRIMER_RIGHT_33_SEQUENCE=GTCCGACCAACAACAATGAC
PRIMER_LEFT_33=579,20
PRIMER_RIGHT_33=1550,20
PRIMER_LEFT_33_TM=59.807
PRIMER_RIGHT_33_TM=58.830
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=5.9000
PRIMER_RIGHT_33_END_STABILITY=6.3000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=972
PRIMER_PAIR_34_PENALTY=1.419683
PRIMER_LEFT_34_PENALTY=0.348633
PRIMER_RIGHT_34_PENALTY=1.071050
PRIMER_LEFT_34_SEQUENCE=AGCTTCCTTGGCCTGATCTT
PRIMER_RIGHT_34_SEQUENCE=AACAATGACGCAGCTTGAAC
PRIMER_LEFT_34=888,20
PRIMER_RIGHT_34=1539,20
PRIMER_LEFT_34_TM=60.349
PRIMER_RIGHT_34_TM=58.929
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.6000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=652
PRIMER_PAIR_35_PENALTY=1.423303
PRIMER_LEFT_35_PENALTY=0.352253
PRIMER_RIGHT_35_PENALTY=1.071050
PRIMER_LEFT_35_SEQUENCE=GGTTTGCATTTCGGAACAAC
PRIMER_RIGHT_35_SEQUENCE=AACAATGACGCAGCTTGAAC
PRIMER_LEFT_35=818,20
PRIMER_RIGHT_35=1539,20
PRIMER_LEFT_35_TM=60.352
PRIMER_RIGHT_35_TM=58.929
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=722
PRIMER_PAIR_36_PENALTY=1.459290
PRIMER_LEFT_36_PENALTY=0.388240
PRIMER_RIGHT_36_PENALTY=1.071050
PRIMER_LEFT_36_SEQUENCE=AACGGGGTTGACTTGTTTCA
PRIMER_RIGHT_36_SEQUENCE=AACAATGACGCAGCTTGAAC
PRIMER_LEFT_36=737,20
PRIMER_RIGHT_36=1539,20
PRIMER_LEFT_36_TM=60.388
PRIMER_RIGHT_36_TM=58.929
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=803
PRIMER_PAIR_37_PENALTY=1.459290
PRIMER_LEFT_37_PENALTY=0.388240
PRIMER_RIGHT_37_PENALTY=1.071050
PRIMER_LEFT_37_SEQUENCE=CAACGGGGTTGACTTGTTTC
PRIMER_RIGHT_37_SEQUENCE=AACAATGACGCAGCTTGAAC
PRIMER_LEFT_37=736,20
PRIMER_RIGHT_37=1539,20
PRIMER_LEFT_37_TM=60.388
PRIMER_RIGHT_37_TM=58.929
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=804
PRIMER_PAIR_38_PENALTY=1.480759
PRIMER_LEFT_38_PENALTY=1.321010
PRIMER_RIGHT_38_PENALTY=0.159749
PRIMER_LEFT_38_SEQUENCE=ACTTCCGTTGGAATCATCCTC
PRIMER_RIGHT_38_SEQUENCE=GACCAACAACAATGACGCAG
PRIMER_LEFT_38=628,21
PRIMER_RIGHT_38=1546,20
PRIMER_LEFT_38_TM=60.321
PRIMER_RIGHT_38_TM=60.160
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=10.2000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=919
PRIMER_PAIR_39_PENALTY=1.504662
PRIMER_LEFT_39_PENALTY=0.433612
PRIMER_RIGHT_39_PENALTY=1.071050
PRIMER_LEFT_39_SEQUENCE=TCAGAAAGCGGCATTAGACA
PRIMER_RIGHT_39_SEQUENCE=AACAATGACGCAGCTTGAAC
PRIMER_LEFT_39=582,20
PRIMER_RIGHT_39=1539,20
PRIMER_LEFT_39_TM=59.566
PRIMER_RIGHT_39_TM=58.929
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=958
PRIMER_PAIR_40_PENALTY=1.509090
PRIMER_LEFT_40_PENALTY=0.712754
PRIMER_RIGHT_40_PENALTY=0.796337
PRIMER_LEFT_40_SEQUENCE=GCTTCCTTGGCCTGATCTTT
PRIMER_RIGHT_40_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_40=889,20
PRIMER_RIGHT_40=1527,20
PRIMER_LEFT_40_TM=60.713
PRIMER_RIGHT_40_TM=60.796
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=5.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=639
PRIMER_PAIR_41_PENALTY=1.509564
PRIMER_LEFT_41_PENALTY=0.713227
PRIMER_RIGHT_41_PENALTY=0.796337
PRIMER_LEFT_41_SEQUENCE=TCCTTGGCCTGATCTTTTGA
PRIMER_RIGHT_41_SEQUENCE=GCTTGAACTGGAGCGGTATG
PRIMER_LEFT_41=892,20
PRIMER_RIGHT_41=1527,20
PRIMER_LEFT_41_TM=60.713
PRIMER_RIGHT_41_TM=60.796
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=5.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=636
PRIMER_PAIR_42_PENALTY=1.518908
PRIMER_LEFT_42_PENALTY=0.348633
PRIMER_RIGHT_42_PENALTY=1.170275
PRIMER_LEFT_42_SEQUENCE=AGCTTCCTTGGCCTGATCTT
PRIMER_RIGHT_42_SEQUENCE=GTCCGACCAACAACAATGAC
PRIMER_LEFT_42=888,20
PRIMER_RIGHT_42=1550,20
PRIMER_LEFT_42_TM=60.349
PRIMER_RIGHT_42_TM=58.830
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=663
PRIMER_PAIR_43_PENALTY=1.522528
PRIMER_LEFT_43_PENALTY=0.352253
PRIMER_RIGHT_43_PENALTY=1.170275
PRIMER_LEFT_43_SEQUENCE=GGTTTGCATTTCGGAACAAC
PRIMER_RIGHT_43_SEQUENCE=GTCCGACCAACAACAATGAC
PRIMER_LEFT_43=818,20
PRIMER_RIGHT_43=1550,20
PRIMER_LEFT_43_TM=60.352
PRIMER_RIGHT_43_TM=58.830
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=733
PRIMER_PAIR_44_PENALTY=1.539350
PRIMER_LEFT_44_PENALTY=0.183944
PRIMER_RIGHT_44_PENALTY=1.355407
PRIMER_LEFT_44_SEQUENCE=TTCCTTGGCCTGATCTTTTG
PRIMER_RIGHT_44_SEQUENCE=TGAATTGGCTGAGATTGGTC
PRIMER_LEFT_44=891,20
PRIMER_RIGHT_44=1854,20
PRIMER_LEFT_44_TM=60.184
PRIMER_RIGHT_44_TM=58.645
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=964
PRIMER_PAIR_45_PENALTY=1.557191
PRIMER_LEFT_45_PENALTY=1.000680
PRIMER_RIGHT_45_PENALTY=0.556510
PRIMER_LEFT_45_SEQUENCE=CAAGCAAGACGGATCAGAAAG
PRIMER_RIGHT_45_SEQUENCE=TGCGCCAGTAAATTGAAAGA
PRIMER_LEFT_45=569,21
PRIMER_RIGHT_45=1509,20
PRIMER_LEFT_45_TM=60.001
PRIMER_RIGHT_45_TM=59.443
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=941
PRIMER_PAIR_46_PENALTY=1.557942
PRIMER_LEFT_46_PENALTY=1.001431
PRIMER_RIGHT_46_PENALTY=0.556510
PRIMER_LEFT_46_SEQUENCE=CAAGCAAGACGGATCAGAAA
PRIMER_RIGHT_46_SEQUENCE=TGCGCCAGTAAATTGAAAGA
PRIMER_LEFT_46=569,20
PRIMER_RIGHT_46=1509,20
PRIMER_LEFT_46_TM=58.999
PRIMER_RIGHT_46_TM=59.443
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=941
PRIMER_PAIR_47_PENALTY=1.558515
PRIMER_LEFT_47_PENALTY=0.388240
PRIMER_RIGHT_47_PENALTY=1.170275
PRIMER_LEFT_47_SEQUENCE=AACGGGGTTGACTTGTTTCA
PRIMER_RIGHT_47_SEQUENCE=GTCCGACCAACAACAATGAC
PRIMER_LEFT_47=737,20
PRIMER_RIGHT_47=1550,20
PRIMER_LEFT_47_TM=60.388
PRIMER_RIGHT_47_TM=58.830
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.3000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=814
PRIMER_PAIR_48_PENALTY=1.558515
PRIMER_LEFT_48_PENALTY=0.388240
PRIMER_RIGHT_48_PENALTY=1.170275
PRIMER_LEFT_48_SEQUENCE=CAACGGGGTTGACTTGTTTC
PRIMER_RIGHT_48_SEQUENCE=GTCCGACCAACAACAATGAC
PRIMER_LEFT_48=736,20
PRIMER_RIGHT_48=1550,20
PRIMER_LEFT_48_TM=60.388
PRIMER_RIGHT_48_TM=58.830
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=815
PRIMER_PAIR_49_PENALTY=1.587725
PRIMER_LEFT_49_PENALTY=0.516675
PRIMER_RIGHT_49_PENALTY=1.071050
PRIMER_LEFT_49_SEQUENCE=CTTCCGTTGGAATCATCCTC
PRIMER_RIGHT_49_SEQUENCE=AACAATGACGCAGCTTGAAC
PRIMER_LEFT_49=629,20
PRIMER_RIGHT_49=1539,20
PRIMER_LEFT_49_TM=59.483
PRIMER_RIGHT_49_TM=58.929
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=911
=
SEQUENCE_ID=MAGIC.446_4_1626847_1627221_edi-0_wu-0_798_282082_TRUE
SEQUENCE_TARGET=950,474
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_22252745_22252850_ler-0_can-0_392454_371892_TRUE
SEQUENCE_TARGET=950,205
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACATANTTTGGTTTCAGTATTTGTAGGNCCAACTTGGACAACAAATACTCAAAGCTTGAACANCCNGTGCTCTAGTGAAACCGTCCCGAAGATAGTGAGACCGTTTTCTGTTTTCTTGTNNNNNNNNCTCGGACAATAAAATNAATTCATTCTGGCTACTTATCAAAGAAGTCTTATNATAGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAACTTCNTATCTAGAAGAATTAAGCATCNAACTGATAGANTAANTTATATCTTCATCTTCCTTAAAATTCACTGTGACNAGGGCNTAANGTCTTGGCGAGGCTCTTTNTATNTAAAGNNTTTTNCTGGTTTGTCATTGATNTCNATTTGTTTACATCAAATCAAACCAACAGAGNNNNTTATAAGGAGAAAANTATATGTTAATTNNNNNAATNGAATCACTNTTTATTTCAATATCTATCCAAAANTTTGAAATAAGAAAANNCATTCAANATTAAATTTTNTATCCACTGGTCTTAAACAAANTTNTGTCAATACAACCATACATTCTATAATCTAATTGACTACATACTAATTNNNNNNNNNNNNTATATATATNNCGTGATCTTATTATGAAGTGATTGCCGGATGNTATAGGAAAGTTGCAACGTCTAAAACATCTCAATTTGGANATGACGAAGAGTATTGATGAGATATCTTNAATTCAAACTTTTCGTCCGATGCTAGCACAATAGAGGAGNTGCATTAACGATTGGAACTACTAGAAATCTGAAACATAATATGTCCTAAGTTNAAGAATTTATAAACGCACCTTATAACTTATAACNTTTANATAAAATCTAAACTTNAGGAAAACTGCGGACTATCTAACTACAAAAAGAACAATGAGACAAATATTTTATCTGGGAAACAAATATGCAAANATAACATTNTTGGAATGCTCTAAAAAAACTAATTATTATTTTTGAATATAAGGACACAATTCTCNTTTTGAANCATAAACGANTGACAAGTTGAGATTGAGTTGCCGACGACTTNACTATGATTAAACGAGTGTNTGGGNTTAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.329177
PRIMER_LEFT_0_PENALTY=0.066323
PRIMER_RIGHT_0_PENALTY=0.262854
PRIMER_LEFT_0_SEQUENCE=TGAAACCGTCCCGAAGATAG
PRIMER_RIGHT_0_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_0=832,20
PRIMER_RIGHT_0=1810,20
PRIMER_LEFT_0_TM=60.066
PRIMER_RIGHT_0_TM=60.263
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=979
PRIMER_PAIR_1_PENALTY=0.335696
PRIMER_LEFT_1_PENALTY=0.209870
PRIMER_RIGHT_1_PENALTY=0.125826
PRIMER_LEFT_1_SEQUENCE=CCGTCCCGAAGATAGTGAGA
PRIMER_RIGHT_1_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_1=837,20
PRIMER_RIGHT_1=1809,20
PRIMER_LEFT_1_TM=60.210
PRIMER_RIGHT_1_TM=59.874
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=0.380439
PRIMER_LEFT_2_PENALTY=0.254613
PRIMER_RIGHT_2_PENALTY=0.125826
PRIMER_LEFT_2_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_2_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_2=824,20
PRIMER_RIGHT_2=1809,20
PRIMER_LEFT_2_TM=60.255
PRIMER_RIGHT_2_TM=59.874
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=986
PRIMER_PAIR_3_PENALTY=0.472724
PRIMER_LEFT_3_PENALTY=0.209870
PRIMER_RIGHT_3_PENALTY=0.262854
PRIMER_LEFT_3_SEQUENCE=CCGTCCCGAAGATAGTGAGA
PRIMER_RIGHT_3_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_3=837,20
PRIMER_RIGHT_3=1810,20
PRIMER_LEFT_3_TM=60.210
PRIMER_RIGHT_3_TM=60.263
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=974
PRIMER_PAIR_4_PENALTY=0.517467
PRIMER_LEFT_4_PENALTY=0.254613
PRIMER_RIGHT_4_PENALTY=0.262854
PRIMER_LEFT_4_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_4_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_4=824,20
PRIMER_RIGHT_4=1810,20
PRIMER_LEFT_4_TM=60.255
PRIMER_RIGHT_4_TM=60.263
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=987
PRIMER_PAIR_5_PENALTY=0.575656
PRIMER_LEFT_5_PENALTY=0.449829
PRIMER_RIGHT_5_PENALTY=0.125826
PRIMER_LEFT_5_SEQUENCE=AACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_5_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_5=835,20
PRIMER_RIGHT_5=1809,20
PRIMER_LEFT_5_TM=59.550
PRIMER_RIGHT_5_TM=59.874
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=975
PRIMER_PAIR_6_PENALTY=0.585745
PRIMER_LEFT_6_PENALTY=0.066323
PRIMER_RIGHT_6_PENALTY=0.519421
PRIMER_LEFT_6_SEQUENCE=TGAAACCGTCCCGAAGATAG
PRIMER_RIGHT_6_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_6=832,20
PRIMER_RIGHT_6=1500,20
PRIMER_LEFT_6_TM=60.066
PRIMER_RIGHT_6_TM=59.481
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=669
PRIMER_PAIR_7_PENALTY=0.712684
PRIMER_LEFT_7_PENALTY=0.449829
PRIMER_RIGHT_7_PENALTY=0.262854
PRIMER_LEFT_7_SEQUENCE=AACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_7_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_7=835,20
PRIMER_RIGHT_7=1810,20
PRIMER_LEFT_7_TM=59.550
PRIMER_RIGHT_7_TM=60.263
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=976
PRIMER_PAIR_8_PENALTY=0.729291
PRIMER_LEFT_8_PENALTY=0.209870
PRIMER_RIGHT_8_PENALTY=0.519421
PRIMER_LEFT_8_SEQUENCE=CCGTCCCGAAGATAGTGAGA
PRIMER_RIGHT_8_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_8=837,20
PRIMER_RIGHT_8=1500,20
PRIMER_LEFT_8_TM=60.210
PRIMER_RIGHT_8_TM=59.481
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=7.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=664
PRIMER_PAIR_9_PENALTY=0.752242
PRIMER_LEFT_9_PENALTY=0.626415
PRIMER_RIGHT_9_PENALTY=0.125826
PRIMER_LEFT_9_SEQUENCE=TAGTGAAACCGTCCCGAAGA
PRIMER_RIGHT_9_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_9=829,20
PRIMER_RIGHT_9=1809,20
PRIMER_LEFT_9_TM=60.626
PRIMER_RIGHT_9_TM=59.874
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=981
PRIMER_PAIR_10_PENALTY=0.752242
PRIMER_LEFT_10_PENALTY=0.626415
PRIMER_RIGHT_10_PENALTY=0.125826
PRIMER_LEFT_10_SEQUENCE=TCTAGTGAAACCGTCCCGAA
PRIMER_RIGHT_10_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_10=827,20
PRIMER_RIGHT_10=1809,20
PRIMER_LEFT_10_TM=60.626
PRIMER_RIGHT_10_TM=59.874
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=10.2000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=983
PRIMER_PAIR_11_PENALTY=0.774035
PRIMER_LEFT_11_PENALTY=0.254613
PRIMER_RIGHT_11_PENALTY=0.519421
PRIMER_LEFT_11_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_11_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_11=824,20
PRIMER_RIGHT_11=1500,20
PRIMER_LEFT_11_TM=60.255
PRIMER_RIGHT_11_TM=59.481
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.1000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=677
PRIMER_PAIR_12_PENALTY=0.878206
PRIMER_LEFT_12_PENALTY=0.066323
PRIMER_RIGHT_12_PENALTY=0.811883
PRIMER_LEFT_12_SEQUENCE=TGAAACCGTCCCGAAGATAG
PRIMER_RIGHT_12_SEQUENCE=GTCGTCGGCAACTCAATCTC
PRIMER_LEFT_12=832,20
PRIMER_RIGHT_12=1808,20
PRIMER_LEFT_12_TM=60.066
PRIMER_RIGHT_12_TM=60.812
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=5.6000
PRIMER_RIGHT_12_END_STABILITY=6.3000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=977
PRIMER_PAIR_13_PENALTY=0.889270
PRIMER_LEFT_13_PENALTY=0.626415
PRIMER_RIGHT_13_PENALTY=0.262854
PRIMER_LEFT_13_SEQUENCE=TAGTGAAACCGTCCCGAAGA
PRIMER_RIGHT_13_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_13=829,20
PRIMER_RIGHT_13=1810,20
PRIMER_LEFT_13_TM=60.626
PRIMER_RIGHT_13_TM=60.263
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=982
PRIMER_PAIR_14_PENALTY=0.889270
PRIMER_LEFT_14_PENALTY=0.626415
PRIMER_RIGHT_14_PENALTY=0.262854
PRIMER_LEFT_14_SEQUENCE=TCTAGTGAAACCGTCCCGAA
PRIMER_RIGHT_14_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_14=827,20
PRIMER_RIGHT_14=1810,20
PRIMER_LEFT_14_TM=60.626
PRIMER_RIGHT_14_TM=60.263
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=10.2000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=984
PRIMER_PAIR_15_PENALTY=0.969251
PRIMER_LEFT_15_PENALTY=0.449829
PRIMER_RIGHT_15_PENALTY=0.519421
PRIMER_LEFT_15_SEQUENCE=AACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_15_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_15=835,20
PRIMER_RIGHT_15=1500,20
PRIMER_LEFT_15_TM=59.550
PRIMER_RIGHT_15_TM=59.481
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=666
PRIMER_PAIR_16_PENALTY=1.066496
PRIMER_LEFT_16_PENALTY=0.254613
PRIMER_RIGHT_16_PENALTY=0.811883
PRIMER_LEFT_16_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_16_SEQUENCE=GTCGTCGGCAACTCAATCTC
PRIMER_LEFT_16=824,20
PRIMER_RIGHT_16=1808,20
PRIMER_LEFT_16_TM=60.255
PRIMER_RIGHT_16_TM=60.812
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.1000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=985
PRIMER_PAIR_17_PENALTY=1.100790
PRIMER_LEFT_17_PENALTY=0.837936
PRIMER_RIGHT_17_PENALTY=0.262854
PRIMER_LEFT_17_SEQUENCE=ACCGTCCCGAAGATAGTGAG
PRIMER_RIGHT_17_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_17=836,20
PRIMER_RIGHT_17=1810,20
PRIMER_LEFT_17_TM=59.162
PRIMER_RIGHT_17_TM=60.263
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=975
PRIMER_PAIR_18_PENALTY=1.145837
PRIMER_LEFT_18_PENALTY=0.626415
PRIMER_RIGHT_18_PENALTY=0.519421
PRIMER_LEFT_18_SEQUENCE=TAGTGAAACCGTCCCGAAGA
PRIMER_RIGHT_18_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_18=829,20
PRIMER_RIGHT_18=1500,20
PRIMER_LEFT_18_TM=60.626
PRIMER_RIGHT_18_TM=59.481
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=672
PRIMER_PAIR_19_PENALTY=1.145837
PRIMER_LEFT_19_PENALTY=0.626415
PRIMER_RIGHT_19_PENALTY=0.519421
PRIMER_LEFT_19_SEQUENCE=TCTAGTGAAACCGTCCCGAA
PRIMER_RIGHT_19_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_19=827,20
PRIMER_RIGHT_19=1500,20
PRIMER_LEFT_19_TM=60.626
PRIMER_RIGHT_19_TM=59.481
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=10.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=674
PRIMER_PAIR_20_PENALTY=1.210630
PRIMER_LEFT_20_PENALTY=0.947776
PRIMER_RIGHT_20_PENALTY=0.262854
PRIMER_LEFT_20_SEQUENCE=AAACCGTCCCGAAGATAGTG
PRIMER_RIGHT_20_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_20=834,20
PRIMER_RIGHT_20=1810,20
PRIMER_LEFT_20_TM=59.052
PRIMER_RIGHT_20_TM=60.263
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=5.7000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=977
PRIMER_PAIR_21_PENALTY=1.215242
PRIMER_LEFT_21_PENALTY=0.066323
PRIMER_RIGHT_21_PENALTY=1.148919
PRIMER_LEFT_21_SEQUENCE=TGAAACCGTCCCGAAGATAG
PRIMER_RIGHT_21_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_21=832,20
PRIMER_RIGHT_21=1496,20
PRIMER_LEFT_21_TM=60.066
PRIMER_RIGHT_21_TM=61.149
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=5.6000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=665
PRIMER_PAIR_22_PENALTY=1.357357
PRIMER_LEFT_22_PENALTY=0.837936
PRIMER_RIGHT_22_PENALTY=0.519421
PRIMER_LEFT_22_SEQUENCE=ACCGTCCCGAAGATAGTGAG
PRIMER_RIGHT_22_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_22=836,20
PRIMER_RIGHT_22=1500,20
PRIMER_LEFT_22_TM=59.162
PRIMER_RIGHT_22_TM=59.481
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=665
PRIMER_PAIR_23_PENALTY=1.358789
PRIMER_LEFT_23_PENALTY=0.209870
PRIMER_RIGHT_23_PENALTY=1.148919
PRIMER_LEFT_23_SEQUENCE=CCGTCCCGAAGATAGTGAGA
PRIMER_RIGHT_23_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_23=837,20
PRIMER_RIGHT_23=1496,20
PRIMER_LEFT_23_TM=60.210
PRIMER_RIGHT_23_TM=61.149
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=660
PRIMER_PAIR_24_PENALTY=1.403532
PRIMER_LEFT_24_PENALTY=0.254613
PRIMER_RIGHT_24_PENALTY=1.148919
PRIMER_LEFT_24_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_24_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_24=824,20
PRIMER_RIGHT_24=1496,20
PRIMER_LEFT_24_TM=60.255
PRIMER_RIGHT_24_TM=61.149
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.1000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=673
PRIMER_PAIR_25_PENALTY=1.438298
PRIMER_LEFT_25_PENALTY=0.626415
PRIMER_RIGHT_25_PENALTY=0.811883
PRIMER_LEFT_25_SEQUENCE=TAGTGAAACCGTCCCGAAGA
PRIMER_RIGHT_25_SEQUENCE=GTCGTCGGCAACTCAATCTC
PRIMER_LEFT_25=829,20
PRIMER_RIGHT_25=1808,20
PRIMER_LEFT_25_TM=60.626
PRIMER_RIGHT_25_TM=60.812
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=6.3000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=980
PRIMER_PAIR_26_PENALTY=1.438298
PRIMER_LEFT_26_PENALTY=0.626415
PRIMER_RIGHT_26_PENALTY=0.811883
PRIMER_LEFT_26_SEQUENCE=TCTAGTGAAACCGTCCCGAA
PRIMER_RIGHT_26_SEQUENCE=GTCGTCGGCAACTCAATCTC
PRIMER_LEFT_26=827,20
PRIMER_RIGHT_26=1808,20
PRIMER_LEFT_26_TM=60.626
PRIMER_RIGHT_26_TM=60.812
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.2000
PRIMER_RIGHT_26_END_STABILITY=6.3000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=982
PRIMER_PAIR_27_PENALTY=1.467197
PRIMER_LEFT_27_PENALTY=0.947776
PRIMER_RIGHT_27_PENALTY=0.519421
PRIMER_LEFT_27_SEQUENCE=AAACCGTCCCGAAGATAGTG
PRIMER_RIGHT_27_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_27=834,20
PRIMER_RIGHT_27=1500,20
PRIMER_LEFT_27_TM=59.052
PRIMER_RIGHT_27_TM=59.481
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=5.7000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=667
PRIMER_PAIR_28_PENALTY=1.598749
PRIMER_LEFT_28_PENALTY=0.449829
PRIMER_RIGHT_28_PENALTY=1.148919
PRIMER_LEFT_28_SEQUENCE=AACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_28_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_28=835,20
PRIMER_RIGHT_28=1496,20
PRIMER_LEFT_28_TM=59.550
PRIMER_RIGHT_28_TM=61.149
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=662
PRIMER_PAIR_29_PENALTY=1.623537
PRIMER_LEFT_29_PENALTY=0.066323
PRIMER_RIGHT_29_PENALTY=1.557214
PRIMER_LEFT_29_SEQUENCE=TGAAACCGTCCCGAAGATAG
PRIMER_RIGHT_29_SEQUENCE=TCGGCAACTCAATCTCAACT
PRIMER_LEFT_29=832,20
PRIMER_RIGHT_29=1804,20
PRIMER_LEFT_29_TM=60.066
PRIMER_RIGHT_29_TM=58.443
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=5.6000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=973
PRIMER_PAIR_30_PENALTY=1.719007
PRIMER_LEFT_30_PENALTY=0.066323
PRIMER_RIGHT_30_PENALTY=1.652683
PRIMER_LEFT_30_SEQUENCE=TGAAACCGTCCCGAAGATAG
PRIMER_RIGHT_30_SEQUENCE=GTCGGCAACTCAATCTCAACT
PRIMER_LEFT_30=832,20
PRIMER_RIGHT_30=1805,21
PRIMER_LEFT_30_TM=60.066
PRIMER_RIGHT_30_TM=59.347
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=5.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=974
PRIMER_PAIR_31_PENALTY=1.759658
PRIMER_LEFT_31_PENALTY=0.947776
PRIMER_RIGHT_31_PENALTY=0.811883
PRIMER_LEFT_31_SEQUENCE=AAACCGTCCCGAAGATAGTG
PRIMER_RIGHT_31_SEQUENCE=GTCGTCGGCAACTCAATCTC
PRIMER_LEFT_31=834,20
PRIMER_RIGHT_31=1808,20
PRIMER_LEFT_31_TM=59.052
PRIMER_RIGHT_31_TM=60.812
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.7000
PRIMER_RIGHT_31_END_STABILITY=6.3000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=975
PRIMER_PAIR_32_PENALTY=1.764962
PRIMER_LEFT_32_PENALTY=1.502108
PRIMER_RIGHT_32_PENALTY=0.262854
PRIMER_LEFT_32_SEQUENCE=AACCGTCCCGAAGATAGTGAG
PRIMER_RIGHT_32_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_32=835,21
PRIMER_RIGHT_32=1810,20
PRIMER_LEFT_32_TM=60.502
PRIMER_RIGHT_32_TM=60.263
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=976
PRIMER_PAIR_33_PENALTY=1.775335
PRIMER_LEFT_33_PENALTY=0.626415
PRIMER_RIGHT_33_PENALTY=1.148919
PRIMER_LEFT_33_SEQUENCE=TAGTGAAACCGTCCCGAAGA
PRIMER_RIGHT_33_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_33=829,20
PRIMER_RIGHT_33=1496,20
PRIMER_LEFT_33_TM=60.626
PRIMER_RIGHT_33_TM=61.149
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=668
PRIMER_PAIR_34_PENALTY=1.775335
PRIMER_LEFT_34_PENALTY=0.626415
PRIMER_RIGHT_34_PENALTY=1.148919
PRIMER_LEFT_34_SEQUENCE=TCTAGTGAAACCGTCCCGAA
PRIMER_RIGHT_34_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_34=827,20
PRIMER_RIGHT_34=1496,20
PRIMER_LEFT_34_TM=60.626
PRIMER_RIGHT_34_TM=61.149
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=10.2000
PRIMER_RIGHT_34_END_STABILITY=7.3000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=670
PRIMER_PAIR_35_PENALTY=1.779173
PRIMER_LEFT_35_PENALTY=0.066323
PRIMER_RIGHT_35_PENALTY=1.712850
PRIMER_LEFT_35_SEQUENCE=TGAAACCGTCCCGAAGATAG
PRIMER_RIGHT_35_SEQUENCE=GTCGGCAACTCAATCTCAAC
PRIMER_LEFT_35=832,20
PRIMER_RIGHT_35=1805,20
PRIMER_LEFT_35_TM=60.066
PRIMER_RIGHT_35_TM=58.287
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=5.6000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=974
PRIMER_PAIR_36_PENALTY=1.811827
PRIMER_LEFT_36_PENALTY=0.254613
PRIMER_RIGHT_36_PENALTY=1.557214
PRIMER_LEFT_36_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_36_SEQUENCE=TCGGCAACTCAATCTCAACT
PRIMER_LEFT_36=824,20
PRIMER_RIGHT_36=1804,20
PRIMER_LEFT_36_TM=60.255
PRIMER_RIGHT_36_TM=58.443
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.1000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=981
PRIMER_PAIR_37_PENALTY=1.907297
PRIMER_LEFT_37_PENALTY=0.254613
PRIMER_RIGHT_37_PENALTY=1.652683
PRIMER_LEFT_37_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_37_SEQUENCE=GTCGGCAACTCAATCTCAACT
PRIMER_LEFT_37=824,20
PRIMER_RIGHT_37=1805,21
PRIMER_LEFT_37_TM=60.255
PRIMER_RIGHT_37_TM=59.347
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.1000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=982
PRIMER_PAIR_38_PENALTY=1.967463
PRIMER_LEFT_38_PENALTY=0.254613
PRIMER_RIGHT_38_PENALTY=1.712850
PRIMER_LEFT_38_SEQUENCE=TGCTCTAGTGAAACCGTCCC
PRIMER_RIGHT_38_SEQUENCE=GTCGGCAACTCAATCTCAAC
PRIMER_LEFT_38=824,20
PRIMER_RIGHT_38=1805,20
PRIMER_LEFT_38_TM=60.255
PRIMER_RIGHT_38_TM=58.287
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.1000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=982
PRIMER_PAIR_39_PENALTY=1.986181
PRIMER_LEFT_39_PENALTY=1.860355
PRIMER_RIGHT_39_PENALTY=0.125826
PRIMER_LEFT_39_SEQUENCE=AAACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_39_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_39=834,21
PRIMER_RIGHT_39=1809,20
PRIMER_LEFT_39_TM=60.860
PRIMER_RIGHT_39_TM=59.874
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=976
PRIMER_PAIR_40_PENALTY=1.986181
PRIMER_LEFT_40_PENALTY=1.860355
PRIMER_RIGHT_40_PENALTY=0.125826
PRIMER_LEFT_40_SEQUENCE=TGAAACCGTCCCGAAGATAGT
PRIMER_RIGHT_40_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_40=832,21
PRIMER_RIGHT_40=1809,20
PRIMER_LEFT_40_TM=60.860
PRIMER_RIGHT_40_TM=59.874
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.3000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=978
PRIMER_PAIR_41_PENALTY=1.986855
PRIMER_LEFT_41_PENALTY=0.837936
PRIMER_RIGHT_41_PENALTY=1.148919
PRIMER_LEFT_41_SEQUENCE=ACCGTCCCGAAGATAGTGAG
PRIMER_RIGHT_41_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_41=836,20
PRIMER_RIGHT_41=1496,20
PRIMER_LEFT_41_TM=59.162
PRIMER_RIGHT_41_TM=61.149
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.3000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=661
PRIMER_PAIR_42_PENALTY=2.004974
PRIMER_LEFT_42_PENALTY=1.879147
PRIMER_RIGHT_42_PENALTY=0.125826
PRIMER_LEFT_42_SEQUENCE=GAAACCGTCCCGAAGATAGT
PRIMER_RIGHT_42_SEQUENCE=AGTCGTCGGCAACTCAATCT
PRIMER_LEFT_42=833,20
PRIMER_RIGHT_42=1809,20
PRIMER_LEFT_42_TM=58.121
PRIMER_RIGHT_42_TM=59.874
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.3000
PRIMER_RIGHT_42_END_STABILITY=6.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=977
PRIMER_PAIR_43_PENALTY=2.007043
PRIMER_LEFT_43_PENALTY=0.449829
PRIMER_RIGHT_43_PENALTY=1.557214
PRIMER_LEFT_43_SEQUENCE=AACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_43_SEQUENCE=TCGGCAACTCAATCTCAACT
PRIMER_LEFT_43=835,20
PRIMER_RIGHT_43=1804,20
PRIMER_LEFT_43_TM=59.550
PRIMER_RIGHT_43_TM=58.443
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=970
PRIMER_PAIR_44_PENALTY=2.021529
PRIMER_LEFT_44_PENALTY=1.502108
PRIMER_RIGHT_44_PENALTY=0.519421
PRIMER_LEFT_44_SEQUENCE=AACCGTCCCGAAGATAGTGAG
PRIMER_RIGHT_44_SEQUENCE=GCTAGCATCGGACGAAAAGT
PRIMER_LEFT_44=835,21
PRIMER_RIGHT_44=1500,20
PRIMER_LEFT_44_TM=60.502
PRIMER_RIGHT_44_TM=59.481
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=7.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=666
PRIMER_PAIR_45_PENALTY=2.096695
PRIMER_LEFT_45_PENALTY=0.947776
PRIMER_RIGHT_45_PENALTY=1.148919
PRIMER_LEFT_45_SEQUENCE=AAACCGTCCCGAAGATAGTG
PRIMER_RIGHT_45_SEQUENCE=GCATCGGACGAAAAGTTTGA
PRIMER_LEFT_45=834,20
PRIMER_RIGHT_45=1496,20
PRIMER_LEFT_45_TM=59.052
PRIMER_RIGHT_45_TM=61.149
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=5.7000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=663
PRIMER_PAIR_46_PENALTY=2.102513
PRIMER_LEFT_46_PENALTY=0.449829
PRIMER_RIGHT_46_PENALTY=1.652683
PRIMER_LEFT_46_SEQUENCE=AACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_46_SEQUENCE=GTCGGCAACTCAATCTCAACT
PRIMER_LEFT_46=835,20
PRIMER_RIGHT_46=1805,21
PRIMER_LEFT_46_TM=59.550
PRIMER_RIGHT_46_TM=59.347
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=971
PRIMER_PAIR_47_PENALTY=2.123209
PRIMER_LEFT_47_PENALTY=1.860355
PRIMER_RIGHT_47_PENALTY=0.262854
PRIMER_LEFT_47_SEQUENCE=AAACCGTCCCGAAGATAGTGA
PRIMER_RIGHT_47_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_47=834,21
PRIMER_RIGHT_47=1810,20
PRIMER_LEFT_47_TM=60.860
PRIMER_RIGHT_47_TM=60.263
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=977
PRIMER_PAIR_48_PENALTY=2.123209
PRIMER_LEFT_48_PENALTY=1.860355
PRIMER_RIGHT_48_PENALTY=0.262854
PRIMER_LEFT_48_SEQUENCE=GAAACCGTCCCGAAGATAGTG
PRIMER_RIGHT_48_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_48=833,21
PRIMER_RIGHT_48=1810,20
PRIMER_LEFT_48_TM=60.860
PRIMER_RIGHT_48_TM=60.263
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.7000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=978
PRIMER_PAIR_49_PENALTY=2.123209
PRIMER_LEFT_49_PENALTY=1.860355
PRIMER_RIGHT_49_PENALTY=0.262854
PRIMER_LEFT_49_SEQUENCE=TGAAACCGTCCCGAAGATAGT
PRIMER_RIGHT_49_SEQUENCE=AAGTCGTCGGCAACTCAATC
PRIMER_LEFT_49=832,21
PRIMER_RIGHT_49=1810,20
PRIMER_LEFT_49_TM=60.860
PRIMER_RIGHT_49_TM=60.263
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.3000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=979
=
SEQUENCE_ID=MAGIC.105_3_11693052_11693364_wu-0_tsu-0_15214_2208013_TRUE
SEQUENCE_TARGET=950,412
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACTGCTTCTAACAATTAAACTTGATCTTNTGTAGCTTTTGNTGAACTTGTTAGGATGCTNATCNTNTGTNGCTTTTNTTNAACTTGTTAAGATGNTANTANTTAGGTTTCACTATGANTTTGTGAATGTAAACTTNATTACTGTTNTTAATGGTATTTAACTAGCATNATTGGCTTTTTAATTCAATATNAATNANGTTTATNATTCCAAATATACNANAATTTGTGANTAAACCAAAACANAAAATGTGACTAACTAGTGANNNAACNACTTNNNNAACNNGTGACNNNTNNGTNACNAAANTTANNCNNANTATGTGANAANTNTGTGANGAATTTGTGACTAAATCAAATTATACAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACNATTTTGCGATGAATTNTANANCGCCTTAACTTCGTCGCAAAAAGGNAAGACTATTTTNCGACNNAATTGTAACNAAGTGTATGAACCGNACCTNNGTNTANCTCGTTGCANTTTAGTCGCATATCTGNNNNNNNNNNNNNNNNNNNNNNNNCAGTCGCATNTTTACGACTANCAGAANGCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNGTAGTCTNNTNCTNTNTCTNAGGTTTTCNGAAAATACTGCNACCTTTTCTCTTAGGTTTTCCNCAANTACTGCANTNTTCATCTATNTCNNTNTCTTCAACGACGAGATTCANAATATCTTCTGTTAGAACATGAAATAAAACAAGTAAAATTCAGGCTNCTTGACCACAAACTTATTGAGTCANTTTANTAGTAAAGAAATNTCTTTACNCANCTGAAAANTCAACTTGCNACTGAGACATATCTTTAGCNTCAGAAGAGACTAATTNCATTTCCTTTGTACCNTTACTTTCTNCAAGAAAAGNAAACTCAAGACCATCGCTTGTATNNAGCATCGGTGACNCATAGACATAACTCGAACACTACTTACTTAGATCATGAACTTCTGAGANTTNTGAAAATAGAAGTGACGAACCACATAAGTAAAATGAACCAATAGATCTAGGGATGTATAATCATAGGAAAAGAGNGAAGAGGTGAAGGAGAGCTAAGGAGAAGGGAGGAATGAAGNAAGAATCAAGGAAGAGGAAGAGNTAANNGAAGAGAGCGAAGAGGATGNAGAAAATAGAAANTAGGGTTTGATTTTTATTTTGAGCTTTTTTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.394719
PRIMER_LEFT_0_PENALTY=0.376347
PRIMER_RIGHT_0_PENALTY=2.018372
PRIMER_LEFT_0_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_0_SEQUENCE=CCCTTCTCCTTAGCTCTCCTTC
PRIMER_LEFT_0=922,20
PRIMER_RIGHT_0=1735,22
PRIMER_LEFT_0_TM=60.376
PRIMER_RIGHT_0_TM=59.982
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=54.545
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=814
PRIMER_PAIR_1_PENALTY=2.394719
PRIMER_LEFT_1_PENALTY=0.376347
PRIMER_RIGHT_1_PENALTY=2.018372
PRIMER_LEFT_1_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_1_SEQUENCE=TCCCTTCTCCTTAGCTCTCCTT
PRIMER_LEFT_1=922,20
PRIMER_RIGHT_1=1736,22
PRIMER_LEFT_1_TM=60.376
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=815
PRIMER_PAIR_2_PENALTY=2.425108
PRIMER_LEFT_2_PENALTY=0.376347
PRIMER_RIGHT_2_PENALTY=2.048761
PRIMER_LEFT_2_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_2_SEQUENCE=CAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_2=922,20
PRIMER_RIGHT_2=1560,20
PRIMER_LEFT_2_TM=60.376
PRIMER_RIGHT_2_TM=57.951
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=639
PRIMER_PAIR_3_PENALTY=2.522829
PRIMER_LEFT_3_PENALTY=0.376347
PRIMER_RIGHT_3_PENALTY=2.146481
PRIMER_LEFT_3_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_3_SEQUENCE=ACAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_3=922,20
PRIMER_RIGHT_3=1561,21
PRIMER_LEFT_3_TM=60.376
PRIMER_RIGHT_3_TM=58.854
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=640
PRIMER_PAIR_4_PENALTY=2.584731
PRIMER_LEFT_4_PENALTY=0.376347
PRIMER_RIGHT_4_PENALTY=2.208384
PRIMER_LEFT_4_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_4_SEQUENCE=CTTCATTCCTCCCTTCTCCTT
PRIMER_LEFT_4=922,20
PRIMER_RIGHT_4=1745,21
PRIMER_LEFT_4_TM=60.376
PRIMER_RIGHT_4_TM=58.792
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=824
PRIMER_PAIR_5_PENALTY=2.606560
PRIMER_LEFT_5_PENALTY=0.376347
PRIMER_RIGHT_5_PENALTY=2.230213
PRIMER_LEFT_5_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_5_SEQUENCE=TTCATTCCTCCCTTCTCCTT
PRIMER_LEFT_5=922,20
PRIMER_RIGHT_5=1744,20
PRIMER_LEFT_5_TM=60.376
PRIMER_RIGHT_5_TM=57.770
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=823
PRIMER_PAIR_6_PENALTY=2.675146
PRIMER_LEFT_6_PENALTY=0.376347
PRIMER_RIGHT_6_PENALTY=2.298799
PRIMER_LEFT_6_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_6_SEQUENCE=TCCCTTCTCCTTAGCTCTCCT
PRIMER_LEFT_6=922,20
PRIMER_RIGHT_6=1736,21
PRIMER_LEFT_6_TM=60.376
PRIMER_RIGHT_6_TM=58.701
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=815
PRIMER_PAIR_7_PENALTY=2.675146
PRIMER_LEFT_7_PENALTY=0.376347
PRIMER_RIGHT_7_PENALTY=2.298799
PRIMER_LEFT_7_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_7_SEQUENCE=CTCCCTTCTCCTTAGCTCTCC
PRIMER_LEFT_7=922,20
PRIMER_RIGHT_7=1737,21
PRIMER_LEFT_7_TM=60.376
PRIMER_RIGHT_7_TM=58.701
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=57.143
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=816
PRIMER_PAIR_8_PENALTY=2.675146
PRIMER_LEFT_8_PENALTY=0.376347
PRIMER_RIGHT_8_PENALTY=2.298799
PRIMER_LEFT_8_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_8_SEQUENCE=CCTCCCTTCTCCTTAGCTCTC
PRIMER_LEFT_8=922,20
PRIMER_RIGHT_8=1738,21
PRIMER_LEFT_8_TM=60.376
PRIMER_RIGHT_8_TM=58.701
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=57.143
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=817
PRIMER_PAIR_9_PENALTY=2.700341
PRIMER_LEFT_9_PENALTY=0.376347
PRIMER_RIGHT_9_PENALTY=2.323994
PRIMER_LEFT_9_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_9_SEQUENCE=TCCCTTCTCCTTAGCTCTCC
PRIMER_LEFT_9=922,20
PRIMER_RIGHT_9=1736,20
PRIMER_LEFT_9_TM=60.376
PRIMER_RIGHT_9_TM=57.676
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=815
PRIMER_PAIR_10_PENALTY=2.739013
PRIMER_LEFT_10_PENALTY=0.376347
PRIMER_RIGHT_10_PENALTY=2.362666
PRIMER_LEFT_10_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_10_SEQUENCE=CTCCCTTCTCCTTAGCTCTCCT
PRIMER_LEFT_10=922,20
PRIMER_RIGHT_10=1737,22
PRIMER_LEFT_10_TM=60.376
PRIMER_RIGHT_10_TM=59.637
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=54.545
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=816
PRIMER_PAIR_11_PENALTY=2.834378
PRIMER_LEFT_11_PENALTY=0.376347
PRIMER_RIGHT_11_PENALTY=2.458031
PRIMER_LEFT_11_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_11_SEQUENCE=TCCTCCCTTCTCCTTAGCTCTC
PRIMER_LEFT_11=922,20
PRIMER_RIGHT_11=1739,22
PRIMER_LEFT_11_TM=60.376
PRIMER_RIGHT_11_TM=60.458
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=54.545
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=818
PRIMER_PAIR_12_PENALTY=2.992640
PRIMER_LEFT_12_PENALTY=0.376347
PRIMER_RIGHT_12_PENALTY=2.616293
PRIMER_LEFT_12_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_12_SEQUENCE=ACAAGCGATGGTCTTGAGTT
PRIMER_LEFT_12=922,20
PRIMER_RIGHT_12=1561,20
PRIMER_LEFT_12_TM=60.376
PRIMER_RIGHT_12_TM=57.384
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=640
PRIMER_PAIR_13_PENALTY=3.003061
PRIMER_LEFT_13_PENALTY=0.376347
PRIMER_RIGHT_13_PENALTY=2.626713
PRIMER_LEFT_13_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_13_SEQUENCE=CTTCATTCCTCCCTTCTCCT
PRIMER_LEFT_13=922,20
PRIMER_RIGHT_13=1745,20
PRIMER_LEFT_13_TM=60.376
PRIMER_RIGHT_13_TM=57.373
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=824
PRIMER_PAIR_14_PENALTY=3.121120
PRIMER_LEFT_14_PENALTY=0.376347
PRIMER_RIGHT_14_PENALTY=2.744773
PRIMER_LEFT_14_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_14_SEQUENCE=CCTTCTCCTTAGCTCTCCTTCA
PRIMER_LEFT_14=922,20
PRIMER_RIGHT_14=1734,22
PRIMER_LEFT_14_TM=60.376
PRIMER_RIGHT_14_TM=59.255
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=813
PRIMER_PAIR_15_PENALTY=3.125905
PRIMER_LEFT_15_PENALTY=0.376347
PRIMER_RIGHT_15_PENALTY=2.749558
PRIMER_LEFT_15_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_15_SEQUENCE=CCCTTCTCCTTAGCTCTCCTT
PRIMER_LEFT_15=922,20
PRIMER_RIGHT_15=1735,21
PRIMER_LEFT_15_TM=60.376
PRIMER_RIGHT_15_TM=58.250
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=814
PRIMER_PAIR_16_PENALTY=3.252326
PRIMER_LEFT_16_PENALTY=0.376347
PRIMER_RIGHT_16_PENALTY=2.875979
PRIMER_LEFT_16_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_16_SEQUENCE=TTCTCCTTAGCTCTCCTTCACC
PRIMER_LEFT_16=922,20
PRIMER_RIGHT_16=1732,22
PRIMER_LEFT_16_TM=60.376
PRIMER_RIGHT_16_TM=59.124
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=811
PRIMER_PAIR_17_PENALTY=3.405286
PRIMER_LEFT_17_PENALTY=0.376347
PRIMER_RIGHT_17_PENALTY=3.028939
PRIMER_LEFT_17_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_17_SEQUENCE=CCTTAGCTCTCCTTCACCTCTTC
PRIMER_LEFT_17=922,20
PRIMER_RIGHT_17=1728,23
PRIMER_LEFT_17_TM=60.376
PRIMER_RIGHT_17_TM=60.029
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=807
PRIMER_PAIR_18_PENALTY=3.405286
PRIMER_LEFT_18_PENALTY=0.376347
PRIMER_RIGHT_18_PENALTY=3.028939
PRIMER_LEFT_18_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_18_SEQUENCE=TCCTTAGCTCTCCTTCACCTCTT
PRIMER_LEFT_18=922,20
PRIMER_RIGHT_18=1729,23
PRIMER_LEFT_18_TM=60.376
PRIMER_RIGHT_18_TM=60.029
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=808
PRIMER_PAIR_19_PENALTY=3.405286
PRIMER_LEFT_19_PENALTY=0.376347
PRIMER_RIGHT_19_PENALTY=3.028939
PRIMER_LEFT_19_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_19_SEQUENCE=TTCTCCTTAGCTCTCCTTCACCT
PRIMER_LEFT_19=922,20
PRIMER_RIGHT_19=1732,23
PRIMER_LEFT_19_TM=60.376
PRIMER_RIGHT_19_TM=60.029
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=811
PRIMER_PAIR_20_PENALTY=3.405286
PRIMER_LEFT_20_PENALTY=0.376347
PRIMER_RIGHT_20_PENALTY=3.028939
PRIMER_LEFT_20_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_20_SEQUENCE=CTTCTCCTTAGCTCTCCTTCACC
PRIMER_LEFT_20=922,20
PRIMER_RIGHT_20=1733,23
PRIMER_LEFT_20_TM=60.376
PRIMER_RIGHT_20_TM=60.029
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=52.174
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=812
PRIMER_PAIR_21_PENALTY=3.405286
PRIMER_LEFT_21_PENALTY=0.376347
PRIMER_RIGHT_21_PENALTY=3.028939
PRIMER_LEFT_21_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_21_SEQUENCE=CCTTCTCCTTAGCTCTCCTTCAC
PRIMER_LEFT_21=922,20
PRIMER_RIGHT_21=1734,23
PRIMER_LEFT_21_TM=60.376
PRIMER_RIGHT_21_TM=60.029
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=52.174
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=813
PRIMER_PAIR_22_PENALTY=3.470142
PRIMER_LEFT_22_PENALTY=0.376347
PRIMER_RIGHT_22_PENALTY=3.093794
PRIMER_LEFT_22_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_22_SEQUENCE=TACAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_22=922,20
PRIMER_RIGHT_22=1562,22
PRIMER_LEFT_22_TM=60.376
PRIMER_RIGHT_22_TM=58.906
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=641
PRIMER_PAIR_23_PENALTY=3.538676
PRIMER_LEFT_23_PENALTY=0.376347
PRIMER_RIGHT_23_PENALTY=3.162328
PRIMER_LEFT_23_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_23_SEQUENCE=CCCTTCTCCTTAGCTCTCCT
PRIMER_LEFT_23=922,20
PRIMER_RIGHT_23=1735,20
PRIMER_LEFT_23_TM=60.376
PRIMER_RIGHT_23_TM=56.838
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=814
PRIMER_PAIR_24_PENALTY=3.615392
PRIMER_LEFT_24_PENALTY=0.376347
PRIMER_RIGHT_24_PENALTY=3.239044
PRIMER_LEFT_24_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_24_SEQUENCE=TCCTTAGCTCTCCTTCACCTCT
PRIMER_LEFT_24=922,20
PRIMER_RIGHT_24=1729,22
PRIMER_LEFT_24_TM=60.376
PRIMER_RIGHT_24_TM=58.761
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=808
PRIMER_PAIR_25_PENALTY=3.615392
PRIMER_LEFT_25_PENALTY=0.376347
PRIMER_RIGHT_25_PENALTY=3.239044
PRIMER_LEFT_25_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_25_SEQUENCE=CTCCTTAGCTCTCCTTCACCTC
PRIMER_LEFT_25=922,20
PRIMER_RIGHT_25=1730,22
PRIMER_LEFT_25_TM=60.376
PRIMER_RIGHT_25_TM=58.761
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=54.545
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=809
PRIMER_PAIR_26_PENALTY=3.615392
PRIMER_LEFT_26_PENALTY=0.376347
PRIMER_RIGHT_26_PENALTY=3.239044
PRIMER_LEFT_26_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_26_SEQUENCE=TCTCCTTAGCTCTCCTTCACCT
PRIMER_LEFT_26=922,20
PRIMER_RIGHT_26=1731,22
PRIMER_LEFT_26_TM=60.376
PRIMER_RIGHT_26_TM=58.761
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=810
PRIMER_PAIR_27_PENALTY=3.629396
PRIMER_LEFT_27_PENALTY=0.376347
PRIMER_RIGHT_27_PENALTY=3.253049
PRIMER_LEFT_27_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_27_SEQUENCE=TCCTTAGCTCTCCTTCACCTC
PRIMER_LEFT_27=922,20
PRIMER_RIGHT_27=1729,21
PRIMER_LEFT_27_TM=60.376
PRIMER_RIGHT_27_TM=57.747
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=808
PRIMER_PAIR_28_PENALTY=3.629396
PRIMER_LEFT_28_PENALTY=0.376347
PRIMER_RIGHT_28_PENALTY=3.253049
PRIMER_LEFT_28_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_28_SEQUENCE=TCTCCTTAGCTCTCCTTCACC
PRIMER_LEFT_28=922,20
PRIMER_RIGHT_28=1731,21
PRIMER_LEFT_28_TM=60.376
PRIMER_RIGHT_28_TM=57.747
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=810
PRIMER_PAIR_29_PENALTY=3.688820
PRIMER_LEFT_29_PENALTY=0.376347
PRIMER_RIGHT_29_PENALTY=3.312473
PRIMER_LEFT_29_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_29_SEQUENCE=CTCCTTAGCTCTCCTTCACCTCT
PRIMER_LEFT_29=922,20
PRIMER_RIGHT_29=1730,23
PRIMER_LEFT_29_TM=60.376
PRIMER_RIGHT_29_TM=59.688
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=52.174
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=809
PRIMER_PAIR_30_PENALTY=3.879124
PRIMER_LEFT_30_PENALTY=0.376347
PRIMER_RIGHT_30_PENALTY=3.502777
PRIMER_LEFT_30_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_30_SEQUENCE=TCTCCTTAGCTCTCCTTCACCTC
PRIMER_LEFT_30=922,20
PRIMER_RIGHT_30=1731,23
PRIMER_LEFT_30_TM=60.376
PRIMER_RIGHT_30_TM=60.503
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=52.174
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=810
PRIMER_PAIR_31_PENALTY=3.879854
PRIMER_LEFT_31_PENALTY=0.376347
PRIMER_RIGHT_31_PENALTY=3.503507
PRIMER_LEFT_31_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_31_SEQUENCE=TACAAGCGATGGTCTTGAGTT
PRIMER_LEFT_31=922,20
PRIMER_RIGHT_31=1562,21
PRIMER_LEFT_31_TM=60.376
PRIMER_RIGHT_31_TM=57.496
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=641
PRIMER_PAIR_32_PENALTY=3.920994
PRIMER_LEFT_32_PENALTY=1.902623
PRIMER_RIGHT_32_PENALTY=2.018372
PRIMER_LEFT_32_SEQUENCE=TTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_32_SEQUENCE=CCCTTCTCCTTAGCTCTCCTTC
PRIMER_LEFT_32=925,20
PRIMER_RIGHT_32=1735,22
PRIMER_LEFT_32_TM=58.097
PRIMER_RIGHT_32_TM=59.982
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=54.545
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=811
PRIMER_PAIR_33_PENALTY=3.920994
PRIMER_LEFT_33_PENALTY=1.902623
PRIMER_RIGHT_33_PENALTY=2.018372
PRIMER_LEFT_33_SEQUENCE=CCTTAACTTCGTCGCAAAAA
PRIMER_RIGHT_33_SEQUENCE=CCCTTCTCCTTAGCTCTCCTTC
PRIMER_LEFT_33=923,20
PRIMER_RIGHT_33=1735,22
PRIMER_LEFT_33_TM=58.097
PRIMER_RIGHT_33_TM=59.982
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=54.545
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.6000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=813
PRIMER_PAIR_34_PENALTY=3.920994
PRIMER_LEFT_34_PENALTY=1.902623
PRIMER_RIGHT_34_PENALTY=2.018372
PRIMER_LEFT_34_SEQUENCE=CCTTAACTTCGTCGCAAAAA
PRIMER_RIGHT_34_SEQUENCE=TCCCTTCTCCTTAGCTCTCCTT
PRIMER_LEFT_34=923,20
PRIMER_RIGHT_34=1736,22
PRIMER_LEFT_34_TM=58.097
PRIMER_RIGHT_34_TM=59.982
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.6000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=814
PRIMER_PAIR_35_PENALTY=3.951383
PRIMER_LEFT_35_PENALTY=1.902623
PRIMER_RIGHT_35_PENALTY=2.048761
PRIMER_LEFT_35_SEQUENCE=TTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_35_SEQUENCE=CAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_35=925,20
PRIMER_RIGHT_35=1560,20
PRIMER_LEFT_35_TM=58.097
PRIMER_RIGHT_35_TM=57.951
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=636
PRIMER_PAIR_36_PENALTY=3.951383
PRIMER_LEFT_36_PENALTY=1.902623
PRIMER_RIGHT_36_PENALTY=2.048761
PRIMER_LEFT_36_SEQUENCE=CCTTAACTTCGTCGCAAAAA
PRIMER_RIGHT_36_SEQUENCE=CAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_36=923,20
PRIMER_RIGHT_36=1560,20
PRIMER_LEFT_36_TM=58.097
PRIMER_RIGHT_36_TM=57.951
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=638
PRIMER_PAIR_37_PENALTY=3.956215
PRIMER_LEFT_37_PENALTY=1.937844
PRIMER_RIGHT_37_PENALTY=2.018372
PRIMER_LEFT_37_SEQUENCE=CTTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_37_SEQUENCE=CCCTTCTCCTTAGCTCTCCTTC
PRIMER_LEFT_37=924,21
PRIMER_RIGHT_37=1735,22
PRIMER_LEFT_37_TM=59.062
PRIMER_RIGHT_37_TM=59.982
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=54.545
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=812
PRIMER_PAIR_38_PENALTY=3.986604
PRIMER_LEFT_38_PENALTY=1.937844
PRIMER_RIGHT_38_PENALTY=2.048761
PRIMER_LEFT_38_SEQUENCE=CTTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_38_SEQUENCE=CAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_38=924,21
PRIMER_RIGHT_38=1560,20
PRIMER_LEFT_38_TM=59.062
PRIMER_RIGHT_38_TM=57.951
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=637
PRIMER_PAIR_39_PENALTY=4.049104
PRIMER_LEFT_39_PENALTY=1.902623
PRIMER_RIGHT_39_PENALTY=2.146481
PRIMER_LEFT_39_SEQUENCE=TTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_39_SEQUENCE=ACAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_39=925,20
PRIMER_RIGHT_39=1561,21
PRIMER_LEFT_39_TM=58.097
PRIMER_RIGHT_39_TM=58.854
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=637
PRIMER_PAIR_40_PENALTY=4.049104
PRIMER_LEFT_40_PENALTY=1.902623
PRIMER_RIGHT_40_PENALTY=2.146481
PRIMER_LEFT_40_SEQUENCE=CCTTAACTTCGTCGCAAAAA
PRIMER_RIGHT_40_SEQUENCE=ACAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_40=923,20
PRIMER_RIGHT_40=1561,21
PRIMER_LEFT_40_TM=58.097
PRIMER_RIGHT_40_TM=58.854
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=639
PRIMER_PAIR_41_PENALTY=4.064043
PRIMER_LEFT_41_PENALTY=0.376347
PRIMER_RIGHT_41_PENALTY=3.687696
PRIMER_LEFT_41_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_41_SEQUENCE=CCTTAGCTCTCCTTCACCTCTT
PRIMER_LEFT_41=922,20
PRIMER_RIGHT_41=1728,22
PRIMER_LEFT_41_TM=60.376
PRIMER_RIGHT_41_TM=58.312
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=807
PRIMER_PAIR_42_PENALTY=4.084325
PRIMER_LEFT_42_PENALTY=1.937844
PRIMER_RIGHT_42_PENALTY=2.146481
PRIMER_LEFT_42_SEQUENCE=CTTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_42_SEQUENCE=ACAAGCGATGGTCTTGAGTTT
PRIMER_LEFT_42=924,21
PRIMER_RIGHT_42=1561,21
PRIMER_LEFT_42_TM=59.062
PRIMER_RIGHT_42_TM=58.854
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=638
PRIMER_PAIR_43_PENALTY=4.111006
PRIMER_LEFT_43_PENALTY=1.902623
PRIMER_RIGHT_43_PENALTY=2.208384
PRIMER_LEFT_43_SEQUENCE=CCTTAACTTCGTCGCAAAAA
PRIMER_RIGHT_43_SEQUENCE=CTTCATTCCTCCCTTCTCCTT
PRIMER_LEFT_43=923,20
PRIMER_RIGHT_43=1745,21
PRIMER_LEFT_43_TM=58.097
PRIMER_RIGHT_43_TM=58.792
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=823
PRIMER_PAIR_44_PENALTY=4.132835
PRIMER_LEFT_44_PENALTY=1.902623
PRIMER_RIGHT_44_PENALTY=2.230213
PRIMER_LEFT_44_SEQUENCE=CCTTAACTTCGTCGCAAAAA
PRIMER_RIGHT_44_SEQUENCE=TTCATTCCTCCCTTCTCCTT
PRIMER_LEFT_44=923,20
PRIMER_RIGHT_44=1744,20
PRIMER_LEFT_44_TM=58.097
PRIMER_RIGHT_44_TM=57.770
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=2.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=822
PRIMER_PAIR_45_PENALTY=4.196467
PRIMER_LEFT_45_PENALTY=0.376347
PRIMER_RIGHT_45_PENALTY=3.820120
PRIMER_LEFT_45_SEQUENCE=GCCTTAACTTCGTCGCAAAA
PRIMER_RIGHT_45_SEQUENCE=CTCCCTTCTCCTTAGCTCTCCTT
PRIMER_LEFT_45=922,20
PRIMER_RIGHT_45=1737,23
PRIMER_LEFT_45_TM=60.376
PRIMER_RIGHT_45_TM=60.820
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=52.174
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=816
PRIMER_PAIR_46_PENALTY=4.201421
PRIMER_LEFT_46_PENALTY=1.902623
PRIMER_RIGHT_46_PENALTY=2.298799
PRIMER_LEFT_46_SEQUENCE=TTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_46_SEQUENCE=TCCCTTCTCCTTAGCTCTCCT
PRIMER_LEFT_46=925,20
PRIMER_RIGHT_46=1736,21
PRIMER_LEFT_46_TM=58.097
PRIMER_RIGHT_46_TM=58.701
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=812
PRIMER_PAIR_47_PENALTY=4.201421
PRIMER_LEFT_47_PENALTY=1.902623
PRIMER_RIGHT_47_PENALTY=2.298799
PRIMER_LEFT_47_SEQUENCE=TTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_47_SEQUENCE=CTCCCTTCTCCTTAGCTCTCC
PRIMER_LEFT_47=925,20
PRIMER_RIGHT_47=1737,21
PRIMER_LEFT_47_TM=58.097
PRIMER_RIGHT_47_TM=58.701
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=57.143
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=813
PRIMER_PAIR_48_PENALTY=4.201421
PRIMER_LEFT_48_PENALTY=1.902623
PRIMER_RIGHT_48_PENALTY=2.298799
PRIMER_LEFT_48_SEQUENCE=TTAACTTCGTCGCAAAAAGG
PRIMER_RIGHT_48_SEQUENCE=CCTCCCTTCTCCTTAGCTCTC
PRIMER_LEFT_48=925,20
PRIMER_RIGHT_48=1738,21
PRIMER_LEFT_48_TM=58.097
PRIMER_RIGHT_48_TM=58.701
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=57.143
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=814
PRIMER_PAIR_49_PENALTY=4.201421
PRIMER_LEFT_49_PENALTY=1.902623
PRIMER_RIGHT_49_PENALTY=2.298799
PRIMER_LEFT_49_SEQUENCE=CCTTAACTTCGTCGCAAAAA
PRIMER_RIGHT_49_SEQUENCE=TCCCTTCTCCTTAGCTCTCCT
PRIMER_LEFT_49=923,20
PRIMER_RIGHT_49=1736,21
PRIMER_LEFT_49_TM=58.097
PRIMER_RIGHT_49_TM=58.701
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=814
=
SEQUENCE_ID=MAGIC.338_3_13982789_13983576_hi-0_no-0_11952_393282_TRUE
SEQUENCE_TARGET=950,887
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_9290275_9290276_kn-0_no-0_700534_137265_TRUE
SEQUENCE_TARGET=950,101
SEQUENCE_TEMPLATE=GTCAAGAACATACACATTGATACAACAAGAAGCCCTCCATAAAATCTCTTCATTTTTCACAGCCGAAAATCACTCAAACGAGAAATCGCGCGGCGAGATCATCAATATTCAATCTTACAGTACAATGTAGCTATTTTTTAACTTCAATCAATCCCTGAAACNCNAAAAAAAAAGTTATGGNTTTTAATCAACATAAGTCATTTTCAAATTTAAGTATAGATTCAGCAGANCCATGATAATGTTAGATTCACGAACTTTATACTAAATNTTNACAAGATCNAANAANATCATCAAANCTTAANAATAANAAGAAGNNNNAAAATCAGATTTTTNTCAGTTAAGTCTNAGAAAATTGAAATTCAACAAAATCNCAATAGACTAGTTAAAATCACTAGAGACNANAAAATCAGAAGTGCCCAGAATTGTTCTCANTTCATAGAAGAACTTACTANACCAACTATATAAGAAAATCAAACNCAGAGCAAAAANNGNAAATCTGCTTACAGGCCAAAGGGAAGCAAAATTAAGCAATGTCTGTTCCGGNATCAGAATCCGGTACACCGGNAAACNGAGAAGTAGAGACGGAAANNTTTGCTGANTGAAATGGGATTCAGATCTCNGAGGCTTTACTAGTTNGCTGCTCTTTGTCGTAGTATCGAAGCACCTTCCACCAGCANAGAAAGCGAAGGTGGTTTCATNTTTCTTTCTNTTTTTTTTNAACTGTAAAANAAGAACGAAAATNGTAAAAACTGTANAGGTNAAAAACANANNNNNAAAGTGAANAAAAGTTACCGATANTTNTTTAGCTANTTTCATACAAAATTATACACAAGGTACTNTAAGAAAATACACAGANATAGTTTCTAACTTNATTCAATACTAATTTAGTAATTTACNANNAAAANAAAAANAANAGTAATTTTANTTACAACNGTANTTTTAAAANATTACNTTTATATTTTGATTTCCTCTGTTTGCATGCTTTGGAAGAAGATNNNNNNNAAAAAAAANTATCACTTGATCACTAAAGTTTTTTGGTTGAGTCTATGACTAAATTACTAAANACATATAAACGAGATCTTTGAATTATCANGATTTGACAANTATGCCTAATCANNNATATTTTTGATGACCCTCNAGNAACATCATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAACAGTAAGNAAAAGTTGNCAAACGAAACTTGAAGGGGTAAAANCAAGTTCCCAAGTCGTGATAGCGNGAGATANGAGGGTGCTTTCATTTGTAACGCACCACCATNNNNNNNNNNNNNACATNCCAGTCATAANTAACNTCTCAATATNTTGATTCCCATTTTTTGCTTTTATGTGCTTTTTTCTTTTGCTGGTTGNNTCGTAGGNANANAANCANAATTGGACTGAAATNTTCNCGTTGTTTTTTTTGTTTTGTNTAATTCACNTAAGNGACTCAAANCAACCTACTCATGATNNNNNNNNNNNNNNACANTCTTTGTTAAACTTACTCATGATGAAAAAANNTAGCCTTGTTGTACGTATATGCTTAAAAANAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.804389
PRIMER_LEFT_0_PENALTY=0.147295
PRIMER_RIGHT_0_PENALTY=0.657094
PRIMER_LEFT_0_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_0_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_0=677,20
PRIMER_RIGHT_0=1277,20
PRIMER_LEFT_0_TM=59.853
PRIMER_RIGHT_0_TM=60.657
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=601
PRIMER_PAIR_1_PENALTY=1.074026
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.926731
PRIMER_LEFT_1_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_1_SEQUENCE=ACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_1=677,20
PRIMER_RIGHT_1=1249,20
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.073
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=573
PRIMER_PAIR_2_PENALTY=1.101560
PRIMER_LEFT_2_PENALTY=0.444466
PRIMER_RIGHT_2_PENALTY=0.657094
PRIMER_LEFT_2_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_2_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_2=652,20
PRIMER_RIGHT_2=1277,20
PRIMER_LEFT_2_TM=59.556
PRIMER_RIGHT_2_TM=60.657
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=626
PRIMER_PAIR_3_PENALTY=1.114593
PRIMER_LEFT_3_PENALTY=0.147295
PRIMER_RIGHT_3_PENALTY=0.967298
PRIMER_LEFT_3_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_3_SEQUENCE=TGGTGGTGCGTTACAAATGA
PRIMER_LEFT_3=677,20
PRIMER_RIGHT_3=1315,20
PRIMER_LEFT_3_TM=59.853
PRIMER_RIGHT_3_TM=60.967
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=639
PRIMER_PAIR_4_PENALTY=1.321418
PRIMER_LEFT_4_PENALTY=0.664324
PRIMER_RIGHT_4_PENALTY=0.657094
PRIMER_LEFT_4_SEQUENCE=ATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_4_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_4=494,20
PRIMER_RIGHT_4=1277,20
PRIMER_LEFT_4_TM=59.336
PRIMER_RIGHT_4_TM=60.657
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=784
PRIMER_PAIR_5_PENALTY=1.371197
PRIMER_LEFT_5_PENALTY=0.444466
PRIMER_RIGHT_5_PENALTY=0.926731
PRIMER_LEFT_5_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_5_SEQUENCE=ACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_5=652,20
PRIMER_RIGHT_5=1249,20
PRIMER_LEFT_5_TM=59.556
PRIMER_RIGHT_5_TM=59.073
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=598
PRIMER_PAIR_6_PENALTY=1.631622
PRIMER_LEFT_6_PENALTY=0.664324
PRIMER_RIGHT_6_PENALTY=0.967298
PRIMER_LEFT_6_SEQUENCE=ATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_6_SEQUENCE=TGGTGGTGCGTTACAAATGA
PRIMER_LEFT_6=494,20
PRIMER_RIGHT_6=1315,20
PRIMER_LEFT_6_TM=59.336
PRIMER_RIGHT_6_TM=60.967
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=822
PRIMER_PAIR_7_PENALTY=1.763675
PRIMER_LEFT_7_PENALTY=1.106581
PRIMER_RIGHT_7_PENALTY=0.657094
PRIMER_LEFT_7_SEQUENCE=GCTTACAGGCCAAAGGGAAG
PRIMER_RIGHT_7_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_7=498,20
PRIMER_RIGHT_7=1277,20
PRIMER_LEFT_7_TM=61.107
PRIMER_RIGHT_7_TM=60.657
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=780
PRIMER_PAIR_8_PENALTY=2.029770
PRIMER_LEFT_8_PENALTY=0.147295
PRIMER_RIGHT_8_PENALTY=1.882474
PRIMER_LEFT_8_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_8_SEQUENCE=TACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_8=677,20
PRIMER_RIGHT_8=1250,21
PRIMER_LEFT_8_TM=59.853
PRIMER_RIGHT_8_TM=59.118
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=574
PRIMER_PAIR_9_PENALTY=2.073879
PRIMER_LEFT_9_PENALTY=1.106581
PRIMER_RIGHT_9_PENALTY=0.967298
PRIMER_LEFT_9_SEQUENCE=GCTTACAGGCCAAAGGGAAG
PRIMER_RIGHT_9_SEQUENCE=TGGTGGTGCGTTACAAATGA
PRIMER_LEFT_9=498,20
PRIMER_RIGHT_9=1315,20
PRIMER_LEFT_9_TM=61.107
PRIMER_RIGHT_9_TM=60.967
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=818
PRIMER_PAIR_10_PENALTY=2.150364
PRIMER_LEFT_10_PENALTY=0.444466
PRIMER_RIGHT_10_PENALTY=1.705897
PRIMER_LEFT_10_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_10_SEQUENCE=GCGTTACAAATGAAAGCACC
PRIMER_LEFT_10=652,20
PRIMER_RIGHT_10=1308,20
PRIMER_LEFT_10_TM=59.556
PRIMER_RIGHT_10_TM=58.294
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=657
PRIMER_PAIR_11_PENALTY=2.167908
PRIMER_LEFT_11_PENALTY=1.510814
PRIMER_RIGHT_11_PENALTY=0.657094
PRIMER_LEFT_11_SEQUENCE=AGTATCGAAGCACCTTCCACC
PRIMER_RIGHT_11_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_11=651,21
PRIMER_RIGHT_11=1277,20
PRIMER_LEFT_11_TM=60.511
PRIMER_RIGHT_11_TM=60.657
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=627
PRIMER_PAIR_12_PENALTY=2.231465
PRIMER_LEFT_12_PENALTY=1.574371
PRIMER_RIGHT_12_PENALTY=0.657094
PRIMER_LEFT_12_SEQUENCE=TATCGAAGCACCTTCCACCA
PRIMER_RIGHT_12_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_12=653,20
PRIMER_RIGHT_12=1277,20
PRIMER_LEFT_12_TM=61.574
PRIMER_RIGHT_12_TM=60.657
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=625
PRIMER_PAIR_13_PENALTY=2.274580
PRIMER_LEFT_13_PENALTY=1.617487
PRIMER_RIGHT_13_PENALTY=0.657094
PRIMER_LEFT_13_SEQUENCE=AATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_13_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_13=493,21
PRIMER_RIGHT_13=1277,20
PRIMER_LEFT_13_TM=60.617
PRIMER_RIGHT_13_TM=60.657
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=785
PRIMER_PAIR_14_PENALTY=2.297280
PRIMER_LEFT_14_PENALTY=0.444466
PRIMER_RIGHT_14_PENALTY=1.852814
PRIMER_LEFT_14_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_14_SEQUENCE=CGTTACAAATGAAAGCACCC
PRIMER_LEFT_14=652,20
PRIMER_RIGHT_14=1307,20
PRIMER_LEFT_14_TM=59.556
PRIMER_RIGHT_14_TM=58.147
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=9.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=656
PRIMER_PAIR_15_PENALTY=2.300228
PRIMER_LEFT_15_PENALTY=0.444466
PRIMER_RIGHT_15_PENALTY=1.855762
PRIMER_LEFT_15_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_15_SEQUENCE=CGTTACAAATGAAAGCACCCT
PRIMER_LEFT_15=652,20
PRIMER_RIGHT_15=1307,21
PRIMER_LEFT_15_TM=59.556
PRIMER_RIGHT_15_TM=59.144
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=9.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=656
PRIMER_PAIR_16_PENALTY=2.326941
PRIMER_LEFT_16_PENALTY=0.444466
PRIMER_RIGHT_16_PENALTY=1.882474
PRIMER_LEFT_16_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_16_SEQUENCE=TACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_16=652,20
PRIMER_RIGHT_16=1250,21
PRIMER_LEFT_16_TM=59.556
PRIMER_RIGHT_16_TM=59.118
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=599
PRIMER_PAIR_17_PENALTY=2.339023
PRIMER_LEFT_17_PENALTY=0.147295
PRIMER_RIGHT_17_PENALTY=2.191728
PRIMER_LEFT_17_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_17_SEQUENCE=ATGGTGGTGCGTTACAAATGA
PRIMER_LEFT_17=677,20
PRIMER_RIGHT_17=1316,21
PRIMER_LEFT_17_TM=59.853
PRIMER_RIGHT_17_TM=61.192
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=640
PRIMER_PAIR_18_PENALTY=2.370222
PRIMER_LEFT_18_PENALTY=0.664324
PRIMER_RIGHT_18_PENALTY=1.705897
PRIMER_LEFT_18_SEQUENCE=ATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_18_SEQUENCE=GCGTTACAAATGAAAGCACC
PRIMER_LEFT_18=494,20
PRIMER_RIGHT_18=1308,20
PRIMER_LEFT_18_TM=59.336
PRIMER_RIGHT_18_TM=58.294
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=815
PRIMER_PAIR_19_PENALTY=2.437545
PRIMER_LEFT_19_PENALTY=1.510814
PRIMER_RIGHT_19_PENALTY=0.926731
PRIMER_LEFT_19_SEQUENCE=AGTATCGAAGCACCTTCCACC
PRIMER_RIGHT_19_SEQUENCE=ACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_19=651,21
PRIMER_RIGHT_19=1249,20
PRIMER_LEFT_19_TM=60.511
PRIMER_RIGHT_19_TM=59.073
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=599
PRIMER_PAIR_20_PENALTY=2.501102
PRIMER_LEFT_20_PENALTY=1.574371
PRIMER_RIGHT_20_PENALTY=0.926731
PRIMER_LEFT_20_SEQUENCE=TATCGAAGCACCTTCCACCA
PRIMER_RIGHT_20_SEQUENCE=ACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_20=653,20
PRIMER_RIGHT_20=1249,20
PRIMER_LEFT_20_TM=61.574
PRIMER_RIGHT_20_TM=59.073
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=597
PRIMER_PAIR_21_PENALTY=2.514925
PRIMER_LEFT_21_PENALTY=0.147295
PRIMER_RIGHT_21_PENALTY=2.367630
PRIMER_LEFT_21_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_21_SEQUENCE=TTACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_21=677,20
PRIMER_RIGHT_21=1251,22
PRIMER_LEFT_21_TM=59.853
PRIMER_RIGHT_21_TM=60.368
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=575
PRIMER_PAIR_22_PENALTY=2.517138
PRIMER_LEFT_22_PENALTY=0.664324
PRIMER_RIGHT_22_PENALTY=1.852814
PRIMER_LEFT_22_SEQUENCE=ATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_22_SEQUENCE=CGTTACAAATGAAAGCACCC
PRIMER_LEFT_22=494,20
PRIMER_RIGHT_22=1307,20
PRIMER_LEFT_22_TM=59.336
PRIMER_RIGHT_22_TM=58.147
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=814
PRIMER_PAIR_23_PENALTY=2.520086
PRIMER_LEFT_23_PENALTY=0.664324
PRIMER_RIGHT_23_PENALTY=1.855762
PRIMER_LEFT_23_SEQUENCE=ATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_23_SEQUENCE=CGTTACAAATGAAAGCACCCT
PRIMER_LEFT_23=494,20
PRIMER_RIGHT_23=1307,21
PRIMER_LEFT_23_TM=59.336
PRIMER_RIGHT_23_TM=59.144
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=9.1000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=814
PRIMER_PAIR_24_PENALTY=2.584784
PRIMER_LEFT_24_PENALTY=1.617487
PRIMER_RIGHT_24_PENALTY=0.967298
PRIMER_LEFT_24_SEQUENCE=AATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_24_SEQUENCE=TGGTGGTGCGTTACAAATGA
PRIMER_LEFT_24=493,21
PRIMER_RIGHT_24=1315,20
PRIMER_LEFT_24_TM=60.617
PRIMER_RIGHT_24_TM=60.967
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=823
PRIMER_PAIR_25_PENALTY=2.636195
PRIMER_LEFT_25_PENALTY=0.444466
PRIMER_RIGHT_25_PENALTY=2.191728
PRIMER_LEFT_25_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_25_SEQUENCE=ATGGTGGTGCGTTACAAATGA
PRIMER_LEFT_25=652,20
PRIMER_RIGHT_25=1316,21
PRIMER_LEFT_25_TM=59.556
PRIMER_RIGHT_25_TM=61.192
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=7.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=665
PRIMER_PAIR_26_PENALTY=2.706425
PRIMER_LEFT_26_PENALTY=0.147295
PRIMER_RIGHT_26_PENALTY=2.559130
PRIMER_LEFT_26_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_26_SEQUENCE=CGCTATCACGACTTGGGAACT
PRIMER_LEFT_26=677,20
PRIMER_RIGHT_26=1277,21
PRIMER_LEFT_26_TM=59.853
PRIMER_RIGHT_26_TM=61.559
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=601
PRIMER_PAIR_27_PENALTY=2.812097
PRIMER_LEFT_27_PENALTY=0.444466
PRIMER_RIGHT_27_PENALTY=2.367630
PRIMER_LEFT_27_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_27_SEQUENCE=TTACCCCTTCAAGTTTCGTTTG
PRIMER_LEFT_27=652,20
PRIMER_RIGHT_27=1251,22
PRIMER_LEFT_27_TM=59.556
PRIMER_RIGHT_27_TM=60.368
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=600
PRIMER_PAIR_28_PENALTY=2.812479
PRIMER_LEFT_28_PENALTY=1.106581
PRIMER_RIGHT_28_PENALTY=1.705897
PRIMER_LEFT_28_SEQUENCE=GCTTACAGGCCAAAGGGAAG
PRIMER_RIGHT_28_SEQUENCE=GCGTTACAAATGAAAGCACC
PRIMER_LEFT_28=498,20
PRIMER_RIGHT_28=1308,20
PRIMER_LEFT_28_TM=61.107
PRIMER_RIGHT_28_TM=58.294
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=811
PRIMER_PAIR_29_PENALTY=2.856053
PRIMER_LEFT_29_PENALTY=0.664324
PRIMER_RIGHT_29_PENALTY=2.191728
PRIMER_LEFT_29_SEQUENCE=ATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_29_SEQUENCE=ATGGTGGTGCGTTACAAATGA
PRIMER_LEFT_29=494,20
PRIMER_RIGHT_29=1316,21
PRIMER_LEFT_29_TM=59.336
PRIMER_RIGHT_29_TM=61.192
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=823
PRIMER_PAIR_30_PENALTY=2.959395
PRIMER_LEFT_30_PENALTY=1.106581
PRIMER_RIGHT_30_PENALTY=1.852814
PRIMER_LEFT_30_SEQUENCE=GCTTACAGGCCAAAGGGAAG
PRIMER_RIGHT_30_SEQUENCE=CGTTACAAATGAAAGCACCC
PRIMER_LEFT_30=498,20
PRIMER_RIGHT_30=1307,20
PRIMER_LEFT_30_TM=61.107
PRIMER_RIGHT_30_TM=58.147
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=810
PRIMER_PAIR_31_PENALTY=2.962343
PRIMER_LEFT_31_PENALTY=1.106581
PRIMER_RIGHT_31_PENALTY=1.855762
PRIMER_LEFT_31_SEQUENCE=GCTTACAGGCCAAAGGGAAG
PRIMER_RIGHT_31_SEQUENCE=CGTTACAAATGAAAGCACCCT
PRIMER_LEFT_31=498,20
PRIMER_RIGHT_31=1307,21
PRIMER_LEFT_31_TM=61.107
PRIMER_RIGHT_31_TM=59.144
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=9.1000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=810
PRIMER_PAIR_32_PENALTY=3.003596
PRIMER_LEFT_32_PENALTY=0.444466
PRIMER_RIGHT_32_PENALTY=2.559130
PRIMER_LEFT_32_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_32_SEQUENCE=CGCTATCACGACTTGGGAACT
PRIMER_LEFT_32=652,20
PRIMER_RIGHT_32=1277,21
PRIMER_LEFT_32_TM=59.556
PRIMER_RIGHT_32_TM=61.559
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=626
PRIMER_PAIR_33_PENALTY=3.129393
PRIMER_LEFT_33_PENALTY=2.472299
PRIMER_RIGHT_33_PENALTY=0.657094
PRIMER_LEFT_33_SEQUENCE=AAATCTGCTTACAGGCCAAAG
PRIMER_RIGHT_33_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_33=492,21
PRIMER_RIGHT_33=1277,20
PRIMER_LEFT_33_TM=58.528
PRIMER_RIGHT_33_TM=60.657
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=786
PRIMER_PAIR_34_PENALTY=3.141999
PRIMER_LEFT_34_PENALTY=2.484905
PRIMER_RIGHT_34_PENALTY=0.657094
PRIMER_LEFT_34_SEQUENCE=AAATCTGCTTACAGGCCAAA
PRIMER_RIGHT_34_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_34=492,20
PRIMER_RIGHT_34=1277,20
PRIMER_LEFT_34_TM=57.515
PRIMER_RIGHT_34_TM=60.657
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=786
PRIMER_PAIR_35_PENALTY=3.159655
PRIMER_LEFT_35_PENALTY=2.502561
PRIMER_RIGHT_35_PENALTY=0.657094
PRIMER_LEFT_35_SEQUENCE=TAGTATCGAAGCACCTTCCACC
PRIMER_RIGHT_35_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_35=650,22
PRIMER_RIGHT_35=1277,20
PRIMER_LEFT_35_TM=60.503
PRIMER_RIGHT_35_TM=60.657
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=628
PRIMER_PAIR_36_PENALTY=3.180593
PRIMER_LEFT_36_PENALTY=0.147295
PRIMER_RIGHT_36_PENALTY=3.033298
PRIMER_LEFT_36_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_36_SEQUENCE=GCTATCACGACTTGGGAACTTG
PRIMER_LEFT_36=677,20
PRIMER_RIGHT_36=1276,22
PRIMER_LEFT_36_TM=59.853
PRIMER_RIGHT_36_TM=61.033
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=600
PRIMER_PAIR_37_PENALTY=3.183757
PRIMER_LEFT_37_PENALTY=0.444466
PRIMER_RIGHT_37_PENALTY=2.739290
PRIMER_LEFT_37_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_37_SEQUENCE=GCGTTACAAATGAAAGCACCC
PRIMER_LEFT_37=652,20
PRIMER_RIGHT_37=1308,21
PRIMER_LEFT_37_TM=59.556
PRIMER_RIGHT_37_TM=61.739
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=657
PRIMER_PAIR_38_PENALTY=3.187184
PRIMER_LEFT_38_PENALTY=2.530091
PRIMER_RIGHT_38_PENALTY=0.657094
PRIMER_LEFT_38_SEQUENCE=ATCGAAGCACCTTCCACCAG
PRIMER_RIGHT_38_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_38=654,20
PRIMER_RIGHT_38=1277,20
PRIMER_LEFT_38_TM=62.530
PRIMER_RIGHT_38_TM=60.657
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=624
PRIMER_PAIR_39_PENALTY=3.213354
PRIMER_LEFT_39_PENALTY=2.556260
PRIMER_RIGHT_39_PENALTY=0.657094
PRIMER_LEFT_39_SEQUENCE=CTGCTTACAGGCCAAAGGGA
PRIMER_RIGHT_39_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_39=496,20
PRIMER_RIGHT_39=1277,20
PRIMER_LEFT_39_TM=62.556
PRIMER_RIGHT_39_TM=60.657
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=782
PRIMER_PAIR_40_PENALTY=3.213354
PRIMER_LEFT_40_PENALTY=2.556260
PRIMER_RIGHT_40_PENALTY=0.657094
PRIMER_LEFT_40_SEQUENCE=TCTGCTTACAGGCCAAAGGG
PRIMER_RIGHT_40_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_40=495,20
PRIMER_RIGHT_40=1277,20
PRIMER_LEFT_40_TM=62.556
PRIMER_RIGHT_40_TM=60.657
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=783
PRIMER_PAIR_41_PENALTY=3.216711
PRIMER_LEFT_41_PENALTY=1.510814
PRIMER_RIGHT_41_PENALTY=1.705897
PRIMER_LEFT_41_SEQUENCE=AGTATCGAAGCACCTTCCACC
PRIMER_RIGHT_41_SEQUENCE=GCGTTACAAATGAAAGCACC
PRIMER_LEFT_41=651,21
PRIMER_RIGHT_41=1308,20
PRIMER_LEFT_41_TM=60.511
PRIMER_RIGHT_41_TM=58.294
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=658
PRIMER_PAIR_42_PENALTY=3.223454
PRIMER_LEFT_42_PENALTY=0.664324
PRIMER_RIGHT_42_PENALTY=2.559130
PRIMER_LEFT_42_SEQUENCE=ATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_42_SEQUENCE=CGCTATCACGACTTGGGAACT
PRIMER_LEFT_42=494,20
PRIMER_RIGHT_42=1277,21
PRIMER_LEFT_42_TM=59.336
PRIMER_RIGHT_42_TM=61.559
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=784
PRIMER_PAIR_43_PENALTY=3.264067
PRIMER_LEFT_43_PENALTY=2.606973
PRIMER_RIGHT_43_PENALTY=0.657094
PRIMER_LEFT_43_SEQUENCE=AAAATCAGAAGTGCCCAGAA
PRIMER_RIGHT_43_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_43=402,20
PRIMER_RIGHT_43=1277,20
PRIMER_LEFT_43_TM=57.393
PRIMER_RIGHT_43_TM=60.657
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=876
PRIMER_PAIR_44_PENALTY=3.280268
PRIMER_LEFT_44_PENALTY=1.574371
PRIMER_RIGHT_44_PENALTY=1.705897
PRIMER_LEFT_44_SEQUENCE=TATCGAAGCACCTTCCACCA
PRIMER_RIGHT_44_SEQUENCE=GCGTTACAAATGAAAGCACC
PRIMER_LEFT_44=653,20
PRIMER_RIGHT_44=1308,20
PRIMER_LEFT_44_TM=61.574
PRIMER_RIGHT_44_TM=58.294
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=656
PRIMER_PAIR_45_PENALTY=3.291966
PRIMER_LEFT_45_PENALTY=0.147295
PRIMER_RIGHT_45_PENALTY=3.144671
PRIMER_LEFT_45_SEQUENCE=AGAAAGCGAAGGTGGTTTCA
PRIMER_RIGHT_45_SEQUENCE=GCTATCACGACTTGGGAACT
PRIMER_LEFT_45=677,20
PRIMER_RIGHT_45=1276,20
PRIMER_LEFT_45_TM=59.853
PRIMER_RIGHT_45_TM=56.855
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.3000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=600
PRIMER_PAIR_46_PENALTY=3.298310
PRIMER_LEFT_46_PENALTY=1.106581
PRIMER_RIGHT_46_PENALTY=2.191728
PRIMER_LEFT_46_SEQUENCE=GCTTACAGGCCAAAGGGAAG
PRIMER_RIGHT_46_SEQUENCE=ATGGTGGTGCGTTACAAATGA
PRIMER_LEFT_46=498,20
PRIMER_RIGHT_46=1316,21
PRIMER_LEFT_46_TM=61.107
PRIMER_RIGHT_46_TM=61.192
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=819
PRIMER_PAIR_47_PENALTY=3.321460
PRIMER_LEFT_47_PENALTY=0.444466
PRIMER_RIGHT_47_PENALTY=2.876993
PRIMER_LEFT_47_SEQUENCE=GTATCGAAGCACCTTCCACC
PRIMER_RIGHT_47_SEQUENCE=CGTTACAAATGAAAGCACCCTC
PRIMER_LEFT_47=652,20
PRIMER_RIGHT_47=1307,22
PRIMER_LEFT_47_TM=59.556
PRIMER_RIGHT_47_TM=60.877
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=656
PRIMER_PAIR_48_PENALTY=3.323384
PRIMER_LEFT_48_PENALTY=1.617487
PRIMER_RIGHT_48_PENALTY=1.705897
PRIMER_LEFT_48_SEQUENCE=AATCTGCTTACAGGCCAAAGG
PRIMER_RIGHT_48_SEQUENCE=GCGTTACAAATGAAAGCACC
PRIMER_LEFT_48=493,21
PRIMER_RIGHT_48=1308,20
PRIMER_LEFT_48_TM=60.617
PRIMER_RIGHT_48_TM=58.294
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=816
PRIMER_PAIR_49_PENALTY=3.363365
PRIMER_LEFT_49_PENALTY=2.706271
PRIMER_RIGHT_49_PENALTY=0.657094
PRIMER_LEFT_49_SEQUENCE=TTTGTCGTAGTATCGAAGCACC
PRIMER_RIGHT_49_SEQUENCE=CGCTATCACGACTTGGGAAC
PRIMER_LEFT_49=643,22
PRIMER_RIGHT_49=1277,20
PRIMER_LEFT_49_TM=59.294
PRIMER_RIGHT_49_TM=60.657
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=635
=
SEQUENCE_ID=MAGIC.433_4_4739499_4739625_ler-0_zu-0_15024_14475_TRUE
SEQUENCE_TARGET=950,226
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4375038_4375070_ws-0_ler-0_46750_5310_TRUE
SEQUENCE_TARGET=950,132
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAAATATATNCANTTCGGTGAAATCTAAGTGATGAGTTCGCTCTTCAAAAGACTAGATTTCATCCCTCAAGGATTTTTCCAAGTTTCACCTCTTGGAAAAATATATTCATTTCGGTGAAATCTAAGTGATGAGTTTGCTCTTCACAAGACTAGATTTCATCCCTCAAGGATTTTTCCAAGTTTCACCTCTGGGAAAAGTATATTCATTTCGGTGAAATCTAAGTGATGAGTTCGCTCTTCACAAGACTAGATTTCATCCCTCAAAGATTTTTCCGAGTTCCATCTCTTGGAAAAATATANTTATTTCAGTGAAATTTAAGTNTTGAGTTCGCTCTTCACAAAACTAGATTTCATCCCTCAAGGATTTTTCCGAGTTCCATCTCTTGGAAAAATATATTCATTTCGGTGAAATTTAAGTGTTGAGTTCGCTCTTCACAAAATTAGATTTCATCCCTCAAAGATTTTTCCAAGTTCCACTGCTTGGAAAAATATATTCATTTCGGTGAAATCTAAGTGTTGAGTTCGCTCTTCACAAGACTAGATTTCATTCCTCATCGANTTTTCCAAGTTCCACCTCTTNNAAATATATTCATTTCGNTGAAATTAAGTTTTGAGCTCGCTCTTCACAAGACAAGATTTCATCCCTCAAGNNGTTTTANAAGNTCCACCTCTCANAAAAATGNATTCATTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4127594_4127968_oy-0_wu-0_17673_197919_TRUE
SEQUENCE_TARGET=950,474
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGNAAGANTTGNTNGNNCGGGTAAAGTTTATANTCTTAATCATGTAGAGCGGCAACAAGCANATCGACATGTATTGGTCAACTGCNNACTACTAGATCACCTNCGAGAGTAAGTTATCTAATTCAAGAACAACTAATTATATTTATGTTTTATCGAAATTCTCTAATGTAGACNTTATGGTTGTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATTNNCATGTTTCCCAANGGACGCACGTAACGGCACGTTGAATTTTTTCACACNNNNNACACATGCATGTAAACCGGTTTGAACNAGTCTTAACCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_20828292_20828305_sf-2_po-0_822097_8539_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNANNNNTNATATCATCTGTCANGAAATANNNNNACAANANAAAAACACGTTTNAGCAGAGATANNNNNNNNNNNNNNNGACACNGNNNNNNNNNNNNNNAGCAAATTCAACACTAACCNTGNTGAGGATNTTCGTGTACAAAGAANCTNGCAGCGACTANCAAATAGCAAAGAATGAGCATCAATCNTTTGAAGTAATTCGATGTCCCTTCCTGCATTACAACAAACACAGCTTCATGNAAGAAAANCTCAAGANNNNATGGTTTCGTGATTAGCNNTCATGAAGATAAAATCAACATTNGTTTCATAATTAGCNTCAAGATTAACTANCTGGAGAAAGAAAGCTACAACAATAACAGTTATGANCAGAGTAGCCGTCTCAAATANCTGGANATTTAGNTCCATTTGTGCACCCATCATCNATCCGATCACCACACAAAAAGGAACCTAAATCCAATNAAACTATCAGACCAAANCTTTTAGAGAATGTTNAGTACTAGACCCAANTTCACATNTCATATNCATACNGCGAACATAGANATNTGAATGGAGGATCCAATAGCCACTCCCAAAGACNGATCCTGTAGAGNAGANTANNAAANTTATAACTAAAGNAAANAAGCAAAGACATCAAAATGAAAGAGACTTNTNTACGTACCAGCTTGTCTTTCATNGCAAACATAATCGCACCTGCATGCTCAGCCGCATTCCCAACTATAGGAAGCAAGATNACACTGATAAAAGAGATTGGTATCTTCCATGAGACCGAAGTACCCTATTACAGATTCAAAAACAANATAACANNNNNNGACAACGTAATAATATAAGATCTTCTTAAGAATGAAATACAGGGTCTTTAGTTACCTCTATGGCATCGACAAGATAGCCCGAAAGAAGAGAGACCCAAGCCGTAAAGATTGAAAGCCAGATGATAGCTTCCCACTTGGAGATCTCAGGATCTTCATNATCGTTTGAAGTTTCTTCGTTNNGGTTAGTTTTCTATAATTTACAGCAGAGATAANTTTTAAGTAGAACTGGCAAGAATCNNNNNNA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4453569_4453832_zu-0_tsu-0_4773_37658_TRUE
SEQUENCE_TARGET=950,363
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_5_10077345_10078079_tsu-0_hi-0_12373_10881_TRUE
SEQUENCE_TARGET=950,834
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_15348914_15349702_hi-0_oy-0_164760_4508_TRUE
SEQUENCE_TARGET=950,888
SEQUENCE_TEMPLATE=CTTGATCAATTNTTAAATTCAATAAACTGAAAGATATATGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_8451775_8452501_po-0_wu-0_9854_279595_TRUE
SEQUENCE_TARGET=950,826
SEQUENCE_TEMPLATE=GAGCCTTGTACAATGTGAGTTTCTACATACNTATTACCCTTNTAANNTCNGCAAGTNTGTGTATCTAATAAAGTCGTGATATNTTCNNAAGGTGTGCTATGGCGCTTGTGCATCAAAGAATAAAACGGATTTTCTATGCTTTTCCAAACNCCACGGCAGGNNGTCTCGGGAGTGTTCATAGACTTCAAGGGGAAAAGAGTTTGAACCATCATTATGCAGTGTTTANAGTTTTGCTGCCTGATGACGCACTTAGACAAATGACCACGGTCTAATGCATTGTTGTAGTCGATTGATAANCAGGTTCTGGGTGAGNNAGCTAATTCTTTGTTCTTGTTCNTTCCAANAATCTNGNATCTTATTGAATTATTATGTAGACCAAAAANTTTCGACTTCTATTACTNTGTNNGGCTCANTACTCTNTAGTCAGAAANTTGTGTTTTAGNGAAACAANAGAATGCAANAAACNAACCGNCNCNAANCAATCCTCACATGAAATAATGCATCTCTACTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAACCTANNTTTCNCACAAANGACTCGTTCGNACAGGNTNACCAACCCCTAATATGAAATATCTTCATGTTAGTCAACACTTTTCANCGAATTTTGTTGAANAANATTNAACTCAATAACTNTTNTTTTGGNGACTTTTTCTAGAGATGANCATAACAACGGAAAAAACNGTCNTTCTTNTTCTTGTTACTACACTTTTCNTGATGATCCAATCGACAANTNTCAAAANNAAACNATTCAAGTATTGGGATCTTGTGATGGATTGGTCTTGATTCAGATCCATGACGATTTTNGGCACATTTACTTGATCAATCCTTNGACTAAAGAACACATNAAACTTTNCAAAGTGNTGCGTNGTTGGTACGCACCTTGNTTACTTTCTTAGNTTACATGACCGTGNATACATGATATTTGTTATTATTAGTTAAACTTATACATATCANCTTGATTANTTTTTTTAGCTTTGTNNTATCCGAAANAACTTTTCATGCATGCTCCGTTAGACCTTTCGATCTGCGGTAATTTCCGTAACGTTNGCGTANCCNGGTTGCAAATTAAATCGCAACCTCAGTATCTTGAAGGCAAATCAANCNTTGNATGNGTTTAGAAACATTTCAAAGTGGTGTTTCGTTGNTACNCATCTNTTGTTGACCGGAAGACAAGAACAATGCNTCCTCCTCTTGACAAANAGANTGAAACAANGTTTCGGGTTTGTTTTGGTAGATTGGTAAANNATTGGTGACGAACAANANTCGANGCNTTNTCATNGATCNNCTCAAGTAANTTTCTCTGAATTNTACGGANCNNATAAATGATGCACATTGCTTTGAGANGCANANNCATTTNGCCNAGACTTTTAGAGGCATCNGTAACANAATCTTCATTTGTAGATCATAAGGNNNNNNNNNNAAGCTTGAATTCTCCATTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTTTGAGACATAGAGACCTACTAAACACACATGTGATANTTATAAGAGATTCTCANAAATTGGTTTGTTACCAACGTTGTGANTTGTNGAGTCGTAAAACNTNTACATGCATCTCTGATTTATATNTCNAAAAGAGAAATATTAGTTCCTCACNTTAAAACTTACCATAATTATTGGCCAATTAAGATTCTTTATTTTCTACTTTCTCTCGTTTAAATTTATCTTTTAACACAAAAATAAAATAAAAATCANTAAGAATTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_7579279_7579672_oy-0_hi-0_359439_102075_TRUE
SEQUENCE_TARGET=950,493
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNTATTTGGNTTTTGTTTAAATCGTCTTNTNATATATTTTGGATCTTGTAAGTTTTGTTTNTGTAAGCCTAGGTGAGATCTGGATTCTAAATCTATTACCGAAAACAGAATCTTTGAAAGTGGATCAACGTGTCCATAAATTTGGTAGCGATNCATATATTATAAATCTAAATACTGTAGGATACAATTTGACACGTAATGTATTGNTGAATTTTAATAGGAATTATTAAACCGCAGGTANACGTCTANTCTATNTNCTTTTGATATTCGTCACTTTAAATANNNNNNNNNNNNNNNNNNNACTTNACTCTTTTATGACTAAATCAAGCNNNNNNNNNNNTNTAAACCGTGTCTAAACCATANATAANNNNAATNTCAAATTCTCAATAATCNTTCTCTTTACTTATAAGTTTNTACCAAACCATATTGGTTCAAACATATNTTAATTGTCACTTGGAACCAATACCCTCAACNTAAACTTTTAATTACTAGANCATGCATGATGNTTTTTTTAATAAGAATATCTATCNAAATATATTGGTTTGATATATGTACCTTTAAAACTAAATGATACATTAGACNCTATAAGATATAATATATTAGCATCTTTTTGAAAATGTCGTCACTTGTTTATAAACTGATAATGTTTTCCTCTTCTGTTAGCATTTTGGGTAGCTCATAACATTCTATAGGAAAAGAAAGAAATACAACTTTAACCAACTCNAGTTTGTGACTTTAGCAGTATAAGGACACCCNAAATGGAATCTCNTTAGCTAAAATGNTTTTTTAAACGTCTCTTTATCTGAATGCAAGACATGTGAATCTTACTAAAATACAAAGCAAAAGACAAANNTAAAATAAACGAAAATCAATAATGGATGAAAGNAAAAAAAGCCGTTTTANCCAAAGTTTTTTGGTTTCAGCTTTAATTGGTCTTGGACAGAAGAGTTTANGTAACGTTAATAGCTTTNAAATTNTNNTTTGANATNTTTTCATATGTTATGCTTTCGGAGAAATTCTATCAAATTGAGTTTCTATTAAATCTCCTTAATTANGAAAACTGGGTGCACATGTGCTTGTCTGTCTCTTCGACTTTNTTTTTTTTGTTAATTAACCAATCTTTATTGTTGTTGTGTTTAAAGTGAAAATGCCTTTTAGTTTTAAGTTATCTGTGAAGTACCAATNACAGGGATTTTGTAAGGAAGCCTTTTATCATCTTTTAGATATCAAAATGATATTTGTATATCCCATGTGCNCCCCTCTCTGNGCANAGCCTANCCATCAACTATCTCAATTATTTAGNACCATTATAATTTCATATATAATGCCCAGTCCNAAAATATAGACAATAAAGCAAAGCGAAAATTATNNNTNTTTTNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_9016894_9017227_wil-2_kn-0_2092_596402_TRUE
SEQUENCE_TARGET=950,433
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATANTGAATATAGNTACNAAATAATATAATGATANATTATAATATGACAAATCACAATTTATATNTTTATGTTGTTCGATTTGTGACTACNTATTTGTGTTTGAAAAGTAGTATGATATGTTTTATTNACTACAAGAAAGACAACATTNAATTCNTTTTTGTTTGAATAACGTAACTNAATCGACCACTAAACTATCATACACGTNCTTAAAAAATGATGTTAAGTTTAATGTGATCCCCAAGTGTTGNAATTGGGTTGAGTAATAAATAGAAGTGGACAAATGGTTANTNANTTATTTTCCTAGCTAAATTCAAGTATTTTTCAAAATTATTTTGTTTCAAANTAAAACTTNAAAGTCATAACAAATTCTANNGAGTTTTAAAAATGACCAACAAATTGAAAGTGTGGTTGTTGCAAATGNAACTTNGTGGAGATACGGTCAGTGACAGCCNNACACGACTCCATCGGTTCGATCAAATACCTCACGTAACCCGCCGATTCACTCGATCGTCTTTATTATTCTTTTCNATTTTCAATCATATTGAGCCTGATTGATAATTACAAGTCTCATTACAAACGTATTAGCAACGGTATACAGTCAATATCCGTCACTAAAAATGCTAATTGCAACGAAAAGTATTCTTCTTTCNCTATGTAACTATAATGTGATATAGAATTAGCCCAAAANAAATTGTNTATGATTAATTAGATCTAAAATAAACNATGGTCGTCATGTGACGTTGATCAANCGATAAAGAGTAGTCTGAAATTTACTAATNAAAGTCTTAATTTGTACTTTTTACGAATTATAGTTTAAATGGACACAAAAGTAAATAGATTAANAAAACTAGAAANACGTGGAAATTTACACAAGCACTATGCATGACTGCCTGCATTCCCTCCAAGAGTTTTCTTACCCTAATNAGTACCGGTTCANGAAAATGAAGGTTCTACAAATTATCAATTATAAGTTCCTAAAGTTCTTTTCTTTCTGTGTGTCCTAGAAGANNGATNCTAATTGATTTATACAATG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_14438409_14438933_no-0_tsu-0_41480_16390_TRUE
SEQUENCE_TARGET=950,624
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_14602027_14602033_sf-2_edi-0_102485_562716_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_14486011_14486019_hi-0_wu-0_382412_423622_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=GATTTCAAGAACCTNANGAAATCCCCTGTTCTCGTGAATATCAGAACAGAGACAGCGCCTTGGAAAATGGCCGTGAGGACTGTGGCTACCATGTGTGAACCGTANAATCTATCGCTCGCGGTGGCGGATGTGGAGGAGGCGAAGATCGAGCAGCCGGATACCGCGGTNGCGATTGTGAGAGTGTAGAGGAAGATAAGGGAGAGACCGGAGAGAGATGGGAAGAGACGGAGGGAGATTGTGAGGAAGATGCAACTTGAGGCTGAGCCTAAGAGGATGTAGTTGCAGAGAAGGAAGATGTTGTGTGTGTTGCCTTTCGATTTCGACATTCCCATTTTCTCGAATTCAAGAAGAGAACTTTGGTTTTTTTTTGGGGTTTTGATGTTTGTTTTTGTGTTTCTTGATAAGTTCACTTTGATTTGGGGTTTATATGCATACATAAGAAACAAAGTTGAGGTAAGAGAAAGTAGAGATTTTGTTGGGATTAACGGTCGTTTTTGTTTTTGAAAATATGACCGTTATGACTTAAGGACGAAGACAGAATAATTCAATTAAGGTGTAACGGCTCGATNACTTTTTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAATATGGAAGCATACACATTTGAGGATCAAATCATCACTAAATCCTTAAGCGGTGAAACTTTAGGCAATGTTATGAAATTAACTTGATATAGAAGACTCCCCTAAACTAAACATGGCTAGAAACAAGAGCAATAAGGGAAAGAGAGATCTTCAAACTGACTAGAAAGCGTAGTGGAAGTCCCTTCACATCAATAAGCTTTATATATAGTTTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4744619_4744623_zu-0_wu-0_4784_37915_TRUE
SEQUENCE_TARGET=950,104
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_8619302_8619720_bur-0_oy-0_347614_522416_TRUE
SEQUENCE_TARGET=950,518
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTGGGTTGTTAAAGCAGCAGCTGGGAAATTTGGTGCATTTGTTGCTGGGAAGGCTATGAAGTGTANTTATNATAGAGGANATAATTATTTTGAGGTTGATGTTGATATAAGTANCTCGGCGATTATNNNNNNNNNNNNTAGGTTTATGTTGGGGTATGTTACGTATTTAATGGTTGATNTTGGTTTTGTTGTGGAAGCGCAAACGGNGGAGGAGTTGCCGGAGAGATTGATTGGTGGTGCTAGGATTTGTCATATGGAGTTNTCNTCGNCGNTTNTGNNNNNNGNTGATGNTGANGNGAAGANGAGTTNNCAGCNACGGCGGAGAATGATGGGTGNGGCGGAAGNNNNNNANANCGANGATGAGNNTTGAGATTTGAAACGAAGATACGTCAACGCTTACNNTTTTTTTTTGTTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGNGTGATACAATGCTAGCTTGTCAACACGATAAGCTGCGAAGTTTCATTGAACTGAAGAGTATAGCTTGTGCGATACATTCAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACNAAGTAAACAAAAACATAATCAGACATATATTNGAGAATCAGCTATTTCTTAAACATGTAANTGATAGCTGATCCGATAGGATATATAAAATCACAATTACTTNCACTTNCTCTNTTGTTGGAGGAACTNGNGAATGCCCATTTAATGAATTGCNTNNNCTGGATTAAACGCAAACCNNTTTCTTCAGCTAGAGCCTTTAAATCCCANTTGTTAAATGGGTTTAGTGTGTTTAGAGTGATATGAATCTCTCCATCTTCGTCTTTCATCATCTCTCTTGCACTCTCCATAAATCCTCCAAATACCTTATTGCGTTTCCTGTGAATTGCACACTCAAATGTTGAATATACANTCATATGNANAATATATATATANNNNNNNNNNNNNNTATACNTATNTATTTTTAAAANTATATATATGAAAGTGGTTCCAAACATATATGGANGTATCAGGAATCCAAGATCTTGTTATNAANTTCNAAATATCAAACTTTTGATATATAAGATCTGAAANAAGCTCATCAAAAACCANACCCTGCATGAGGAAAANTAAAGATGATTATGTCGTAATGANCTAAGCGATCGTCTGATTTCATGGAGTGTACATTAACACCACGGACCACNGTGCAACCAAGTCTCTCCAACTCTTCCACNTTNNCTTTTCCANTGTTGTANTTACGCC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.531372
PRIMER_LEFT_0_PENALTY=0.163658
PRIMER_RIGHT_0_PENALTY=0.367713
PRIMER_LEFT_0_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_0_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_0=837,20
PRIMER_RIGHT_0=1628,20
PRIMER_LEFT_0_TM=59.836
PRIMER_RIGHT_0_TM=59.632
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=792
PRIMER_PAIR_1_PENALTY=0.531372
PRIMER_LEFT_1_PENALTY=0.163658
PRIMER_RIGHT_1_PENALTY=0.367713
PRIMER_LEFT_1_SEQUENCE=TGCTGGGAAGGCTATGAAGT
PRIMER_RIGHT_1_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_1=836,20
PRIMER_RIGHT_1=1628,20
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=59.632
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=793
PRIMER_PAIR_2_PENALTY=0.566960
PRIMER_LEFT_2_PENALTY=0.199246
PRIMER_RIGHT_2_PENALTY=0.367713
PRIMER_LEFT_2_SEQUENCE=CTGGGAAATTTGGTGCATTT
PRIMER_RIGHT_2_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_2=814,20
PRIMER_RIGHT_2=1628,20
PRIMER_LEFT_2_TM=59.801
PRIMER_RIGHT_2_TM=59.632
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=815
PRIMER_PAIR_3_PENALTY=0.581219
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=0.367713
PRIMER_LEFT_3_SEQUENCE=GTTGCTGGGAAGGCTATGAA
PRIMER_RIGHT_3_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_3=834,20
PRIMER_RIGHT_3=1628,20
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=59.632
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=795
PRIMER_PAIR_4_PENALTY=0.699742
PRIMER_LEFT_4_PENALTY=0.332028
PRIMER_RIGHT_4_PENALTY=0.367713
PRIMER_LEFT_4_SEQUENCE=GGGAAATTTGGTGCATTTGT
PRIMER_RIGHT_4_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_4=816,20
PRIMER_RIGHT_4=1628,20
PRIMER_LEFT_4_TM=59.668
PRIMER_RIGHT_4_TM=59.632
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=813
PRIMER_PAIR_5_PENALTY=0.708268
PRIMER_LEFT_5_PENALTY=0.163658
PRIMER_RIGHT_5_PENALTY=0.544610
PRIMER_LEFT_5_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_5_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_5=837,20
PRIMER_RIGHT_5=1627,20
PRIMER_LEFT_5_TM=59.836
PRIMER_RIGHT_5_TM=60.545
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=791
PRIMER_PAIR_6_PENALTY=0.708268
PRIMER_LEFT_6_PENALTY=0.163658
PRIMER_RIGHT_6_PENALTY=0.544610
PRIMER_LEFT_6_SEQUENCE=TGCTGGGAAGGCTATGAAGT
PRIMER_RIGHT_6_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_6=836,20
PRIMER_RIGHT_6=1627,20
PRIMER_LEFT_6_TM=59.836
PRIMER_RIGHT_6_TM=60.545
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=792
PRIMER_PAIR_7_PENALTY=0.711663
PRIMER_LEFT_7_PENALTY=0.343950
PRIMER_RIGHT_7_PENALTY=0.367713
PRIMER_LEFT_7_SEQUENCE=TTGCTGGGAAGGCTATGAAG
PRIMER_RIGHT_7_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_7=835,20
PRIMER_RIGHT_7=1628,20
PRIMER_LEFT_7_TM=60.344
PRIMER_RIGHT_7_TM=59.632
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=794
PRIMER_PAIR_8_PENALTY=0.743856
PRIMER_LEFT_8_PENALTY=0.199246
PRIMER_RIGHT_8_PENALTY=0.544610
PRIMER_LEFT_8_SEQUENCE=CTGGGAAATTTGGTGCATTT
PRIMER_RIGHT_8_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_8=814,20
PRIMER_RIGHT_8=1627,20
PRIMER_LEFT_8_TM=59.801
PRIMER_RIGHT_8_TM=60.545
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=814
PRIMER_PAIR_9_PENALTY=0.758115
PRIMER_LEFT_9_PENALTY=0.213506
PRIMER_RIGHT_9_PENALTY=0.544610
PRIMER_LEFT_9_SEQUENCE=GTTGCTGGGAAGGCTATGAA
PRIMER_RIGHT_9_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_9=834,20
PRIMER_RIGHT_9=1627,20
PRIMER_LEFT_9_TM=60.214
PRIMER_RIGHT_9_TM=60.545
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=794
PRIMER_PAIR_10_PENALTY=0.777340
PRIMER_LEFT_10_PENALTY=0.409627
PRIMER_RIGHT_10_PENALTY=0.367713
PRIMER_LEFT_10_SEQUENCE=ATTTGGTGCATTTGTTGCTG
PRIMER_RIGHT_10_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_10=821,20
PRIMER_RIGHT_10=1628,20
PRIMER_LEFT_10_TM=59.590
PRIMER_RIGHT_10_TM=59.632
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=808
PRIMER_PAIR_11_PENALTY=0.876638
PRIMER_LEFT_11_PENALTY=0.332028
PRIMER_RIGHT_11_PENALTY=0.544610
PRIMER_LEFT_11_SEQUENCE=GGGAAATTTGGTGCATTTGT
PRIMER_RIGHT_11_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_11=816,20
PRIMER_RIGHT_11=1627,20
PRIMER_LEFT_11_TM=59.668
PRIMER_RIGHT_11_TM=60.545
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=812
PRIMER_PAIR_12_PENALTY=0.888559
PRIMER_LEFT_12_PENALTY=0.343950
PRIMER_RIGHT_12_PENALTY=0.544610
PRIMER_LEFT_12_SEQUENCE=TTGCTGGGAAGGCTATGAAG
PRIMER_RIGHT_12_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_12=835,20
PRIMER_RIGHT_12=1627,20
PRIMER_LEFT_12_TM=60.344
PRIMER_RIGHT_12_TM=60.545
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=793
PRIMER_PAIR_13_PENALTY=0.954236
PRIMER_LEFT_13_PENALTY=0.409627
PRIMER_RIGHT_13_PENALTY=0.544610
PRIMER_LEFT_13_SEQUENCE=ATTTGGTGCATTTGTTGCTG
PRIMER_RIGHT_13_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_13=821,20
PRIMER_RIGHT_13=1627,20
PRIMER_LEFT_13_TM=59.590
PRIMER_RIGHT_13_TM=60.545
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=807
PRIMER_PAIR_14_PENALTY=1.195602
PRIMER_LEFT_14_PENALTY=0.163658
PRIMER_RIGHT_14_PENALTY=1.031944
PRIMER_LEFT_14_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_14_SEQUENCE=GAAACTTCGCAGCTTATCGTG
PRIMER_LEFT_14=837,20
PRIMER_RIGHT_14=1632,21
PRIMER_LEFT_14_TM=59.836
PRIMER_RIGHT_14_TM=60.032
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=796
PRIMER_PAIR_15_PENALTY=1.195602
PRIMER_LEFT_15_PENALTY=0.163658
PRIMER_RIGHT_15_PENALTY=1.031944
PRIMER_LEFT_15_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_15_SEQUENCE=TGAAACTTCGCAGCTTATCGT
PRIMER_LEFT_15=837,20
PRIMER_RIGHT_15=1633,21
PRIMER_LEFT_15_TM=59.836
PRIMER_RIGHT_15_TM=60.032
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.0000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=797
PRIMER_PAIR_16_PENALTY=1.231190
PRIMER_LEFT_16_PENALTY=0.199246
PRIMER_RIGHT_16_PENALTY=1.031944
PRIMER_LEFT_16_SEQUENCE=CTGGGAAATTTGGTGCATTT
PRIMER_RIGHT_16_SEQUENCE=GAAACTTCGCAGCTTATCGTG
PRIMER_LEFT_16=814,20
PRIMER_RIGHT_16=1632,21
PRIMER_LEFT_16_TM=59.801
PRIMER_RIGHT_16_TM=60.032
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=819
PRIMER_PAIR_17_PENALTY=1.231190
PRIMER_LEFT_17_PENALTY=0.199246
PRIMER_RIGHT_17_PENALTY=1.031944
PRIMER_LEFT_17_SEQUENCE=CTGGGAAATTTGGTGCATTT
PRIMER_RIGHT_17_SEQUENCE=TGAAACTTCGCAGCTTATCGT
PRIMER_LEFT_17=814,20
PRIMER_RIGHT_17=1633,21
PRIMER_LEFT_17_TM=59.801
PRIMER_RIGHT_17_TM=60.032
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.2000
PRIMER_RIGHT_17_END_STABILITY=8.0000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=820
PRIMER_PAIR_18_PENALTY=1.245450
PRIMER_LEFT_18_PENALTY=0.213506
PRIMER_RIGHT_18_PENALTY=1.031944
PRIMER_LEFT_18_SEQUENCE=GTTGCTGGGAAGGCTATGAA
PRIMER_RIGHT_18_SEQUENCE=GAAACTTCGCAGCTTATCGTG
PRIMER_LEFT_18=834,20
PRIMER_RIGHT_18=1632,21
PRIMER_LEFT_18_TM=60.214
PRIMER_RIGHT_18_TM=60.032
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=799
PRIMER_PAIR_19_PENALTY=1.245450
PRIMER_LEFT_19_PENALTY=0.213506
PRIMER_RIGHT_19_PENALTY=1.031944
PRIMER_LEFT_19_SEQUENCE=GTTGCTGGGAAGGCTATGAA
PRIMER_RIGHT_19_SEQUENCE=TGAAACTTCGCAGCTTATCGT
PRIMER_LEFT_19=834,20
PRIMER_RIGHT_19=1633,21
PRIMER_LEFT_19_TM=60.214
PRIMER_RIGHT_19_TM=60.032
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.0000
PRIMER_PAIR_19_COMPL_ANY=7.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=800
PRIMER_PAIR_20_PENALTY=1.363972
PRIMER_LEFT_20_PENALTY=0.332028
PRIMER_RIGHT_20_PENALTY=1.031944
PRIMER_LEFT_20_SEQUENCE=GGGAAATTTGGTGCATTTGT
PRIMER_RIGHT_20_SEQUENCE=GAAACTTCGCAGCTTATCGTG
PRIMER_LEFT_20=816,20
PRIMER_RIGHT_20=1632,21
PRIMER_LEFT_20_TM=59.668
PRIMER_RIGHT_20_TM=60.032
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=817
PRIMER_PAIR_21_PENALTY=1.363972
PRIMER_LEFT_21_PENALTY=0.332028
PRIMER_RIGHT_21_PENALTY=1.031944
PRIMER_LEFT_21_SEQUENCE=GGGAAATTTGGTGCATTTGT
PRIMER_RIGHT_21_SEQUENCE=TGAAACTTCGCAGCTTATCGT
PRIMER_LEFT_21=816,20
PRIMER_RIGHT_21=1633,21
PRIMER_LEFT_21_TM=59.668
PRIMER_RIGHT_21_TM=60.032
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=818
PRIMER_PAIR_22_PENALTY=1.375894
PRIMER_LEFT_22_PENALTY=0.343950
PRIMER_RIGHT_22_PENALTY=1.031944
PRIMER_LEFT_22_SEQUENCE=TTGCTGGGAAGGCTATGAAG
PRIMER_RIGHT_22_SEQUENCE=GAAACTTCGCAGCTTATCGTG
PRIMER_LEFT_22=835,20
PRIMER_RIGHT_22=1632,21
PRIMER_LEFT_22_TM=60.344
PRIMER_RIGHT_22_TM=60.032
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=798
PRIMER_PAIR_23_PENALTY=1.375894
PRIMER_LEFT_23_PENALTY=0.343950
PRIMER_RIGHT_23_PENALTY=1.031944
PRIMER_LEFT_23_SEQUENCE=TTGCTGGGAAGGCTATGAAG
PRIMER_RIGHT_23_SEQUENCE=TGAAACTTCGCAGCTTATCGT
PRIMER_LEFT_23=835,20
PRIMER_RIGHT_23=1633,21
PRIMER_LEFT_23_TM=60.344
PRIMER_RIGHT_23_TM=60.032
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.0000
PRIMER_PAIR_23_COMPL_ANY=7.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=799
PRIMER_PAIR_24_PENALTY=1.403409
PRIMER_LEFT_24_PENALTY=1.035696
PRIMER_RIGHT_24_PENALTY=0.367713
PRIMER_LEFT_24_SEQUENCE=CTTGGGTTGTTAAAGCAGCA
PRIMER_RIGHT_24_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_24=793,20
PRIMER_RIGHT_24=1628,20
PRIMER_LEFT_24_TM=58.964
PRIMER_RIGHT_24_TM=59.632
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=836
PRIMER_PAIR_25_PENALTY=1.441571
PRIMER_LEFT_25_PENALTY=0.409627
PRIMER_RIGHT_25_PENALTY=1.031944
PRIMER_LEFT_25_SEQUENCE=ATTTGGTGCATTTGTTGCTG
PRIMER_RIGHT_25_SEQUENCE=GAAACTTCGCAGCTTATCGTG
PRIMER_LEFT_25=821,20
PRIMER_RIGHT_25=1632,21
PRIMER_LEFT_25_TM=59.590
PRIMER_RIGHT_25_TM=60.032
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=812
PRIMER_PAIR_26_PENALTY=1.441571
PRIMER_LEFT_26_PENALTY=0.409627
PRIMER_RIGHT_26_PENALTY=1.031944
PRIMER_LEFT_26_SEQUENCE=ATTTGGTGCATTTGTTGCTG
PRIMER_RIGHT_26_SEQUENCE=TGAAACTTCGCAGCTTATCGT
PRIMER_LEFT_26=821,20
PRIMER_RIGHT_26=1633,21
PRIMER_LEFT_26_TM=59.590
PRIMER_RIGHT_26_TM=60.032
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=8.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=813
PRIMER_PAIR_27_PENALTY=1.524607
PRIMER_LEFT_27_PENALTY=1.156894
PRIMER_RIGHT_27_PENALTY=0.367713
PRIMER_LEFT_27_SEQUENCE=TTGTTGCTGGGAAGGCTATG
PRIMER_RIGHT_27_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_27=832,20
PRIMER_RIGHT_27=1628,20
PRIMER_LEFT_27_TM=61.157
PRIMER_RIGHT_27_TM=59.632
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.9000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=797
PRIMER_PAIR_28_PENALTY=1.580305
PRIMER_LEFT_28_PENALTY=1.035696
PRIMER_RIGHT_28_PENALTY=0.544610
PRIMER_LEFT_28_SEQUENCE=CTTGGGTTGTTAAAGCAGCA
PRIMER_RIGHT_28_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_28=793,20
PRIMER_RIGHT_28=1627,20
PRIMER_LEFT_28_TM=58.964
PRIMER_RIGHT_28_TM=60.545
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=835
PRIMER_PAIR_29_PENALTY=1.618741
PRIMER_LEFT_29_PENALTY=0.163658
PRIMER_RIGHT_29_PENALTY=1.455083
PRIMER_LEFT_29_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_29_SEQUENCE=ACTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_29=837,20
PRIMER_RIGHT_29=1629,21
PRIMER_LEFT_29_TM=59.836
PRIMER_RIGHT_29_TM=60.455
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=7.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=793
PRIMER_PAIR_30_PENALTY=1.618741
PRIMER_LEFT_30_PENALTY=0.163658
PRIMER_RIGHT_30_PENALTY=1.455083
PRIMER_LEFT_30_SEQUENCE=TGCTGGGAAGGCTATGAAGT
PRIMER_RIGHT_30_SEQUENCE=ACTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_30=836,20
PRIMER_RIGHT_30=1629,21
PRIMER_LEFT_30_TM=59.836
PRIMER_RIGHT_30_TM=60.455
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=7.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=794
PRIMER_PAIR_31_PENALTY=1.654329
PRIMER_LEFT_31_PENALTY=0.199246
PRIMER_RIGHT_31_PENALTY=1.455083
PRIMER_LEFT_31_SEQUENCE=CTGGGAAATTTGGTGCATTT
PRIMER_RIGHT_31_SEQUENCE=ACTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_31=814,20
PRIMER_RIGHT_31=1629,21
PRIMER_LEFT_31_TM=59.801
PRIMER_RIGHT_31_TM=60.455
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=816
PRIMER_PAIR_32_PENALTY=1.668588
PRIMER_LEFT_32_PENALTY=0.213506
PRIMER_RIGHT_32_PENALTY=1.455083
PRIMER_LEFT_32_SEQUENCE=GTTGCTGGGAAGGCTATGAA
PRIMER_RIGHT_32_SEQUENCE=ACTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_32=834,20
PRIMER_RIGHT_32=1629,21
PRIMER_LEFT_32_TM=60.214
PRIMER_RIGHT_32_TM=60.455
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=7.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=796
PRIMER_PAIR_33_PENALTY=1.701504
PRIMER_LEFT_33_PENALTY=1.156894
PRIMER_RIGHT_33_PENALTY=0.544610
PRIMER_LEFT_33_SEQUENCE=TTGTTGCTGGGAAGGCTATG
PRIMER_RIGHT_33_SEQUENCE=TTCGCAGCTTATCGTGTTGA
PRIMER_LEFT_33=832,20
PRIMER_RIGHT_33=1627,20
PRIMER_LEFT_33_TM=61.157
PRIMER_RIGHT_33_TM=60.545
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=5.9000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=796
PRIMER_PAIR_34_PENALTY=1.787111
PRIMER_LEFT_34_PENALTY=0.332028
PRIMER_RIGHT_34_PENALTY=1.455083
PRIMER_LEFT_34_SEQUENCE=GGGAAATTTGGTGCATTTGT
PRIMER_RIGHT_34_SEQUENCE=ACTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_34=816,20
PRIMER_RIGHT_34=1629,21
PRIMER_LEFT_34_TM=59.668
PRIMER_RIGHT_34_TM=60.455
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=814
PRIMER_PAIR_35_PENALTY=1.799032
PRIMER_LEFT_35_PENALTY=0.343950
PRIMER_RIGHT_35_PENALTY=1.455083
PRIMER_LEFT_35_SEQUENCE=TTGCTGGGAAGGCTATGAAG
PRIMER_RIGHT_35_SEQUENCE=ACTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_35=835,20
PRIMER_RIGHT_35=1629,21
PRIMER_LEFT_35_TM=60.344
PRIMER_RIGHT_35_TM=60.455
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=7.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=795
PRIMER_PAIR_36_PENALTY=1.864709
PRIMER_LEFT_36_PENALTY=0.409627
PRIMER_RIGHT_36_PENALTY=1.455083
PRIMER_LEFT_36_SEQUENCE=ATTTGGTGCATTTGTTGCTG
PRIMER_RIGHT_36_SEQUENCE=ACTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_36=821,20
PRIMER_RIGHT_36=1629,21
PRIMER_LEFT_36_TM=59.590
PRIMER_RIGHT_36_TM=60.455
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=809
PRIMER_PAIR_37_PENALTY=1.963072
PRIMER_LEFT_37_PENALTY=0.163658
PRIMER_RIGHT_37_PENALTY=1.799414
PRIMER_LEFT_37_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_37_SEQUENCE=AAACTTCGCAGCTTATCGTG
PRIMER_LEFT_37=837,20
PRIMER_RIGHT_37=1631,20
PRIMER_LEFT_37_TM=59.836
PRIMER_RIGHT_37_TM=58.201
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=7.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=795
PRIMER_PAIR_38_PENALTY=1.979714
PRIMER_LEFT_38_PENALTY=1.612001
PRIMER_RIGHT_38_PENALTY=0.367713
PRIMER_LEFT_38_SEQUENCE=GTGCATTTGTTGCTGGGAAG
PRIMER_RIGHT_38_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_38=826,20
PRIMER_RIGHT_38=1628,20
PRIMER_LEFT_38_TM=61.612
PRIMER_RIGHT_38_TM=59.632
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=7.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=803
PRIMER_PAIR_39_PENALTY=1.998660
PRIMER_LEFT_39_PENALTY=0.199246
PRIMER_RIGHT_39_PENALTY=1.799414
PRIMER_LEFT_39_SEQUENCE=CTGGGAAATTTGGTGCATTT
PRIMER_RIGHT_39_SEQUENCE=AAACTTCGCAGCTTATCGTG
PRIMER_LEFT_39=814,20
PRIMER_RIGHT_39=1631,20
PRIMER_LEFT_39_TM=59.801
PRIMER_RIGHT_39_TM=58.201
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=8.4000
PRIMER_PAIR_39_COMPL_ANY=7.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=818
PRIMER_PAIR_40_PENALTY=2.012320
PRIMER_LEFT_40_PENALTY=1.644607
PRIMER_RIGHT_40_PENALTY=0.367713
PRIMER_LEFT_40_SEQUENCE=GCTGGGAAGGCTATGAAGTGT
PRIMER_RIGHT_40_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_40=837,21
PRIMER_RIGHT_40=1628,20
PRIMER_LEFT_40_TM=60.645
PRIMER_RIGHT_40_TM=59.632
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.1000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=792
PRIMER_PAIR_41_PENALTY=2.012919
PRIMER_LEFT_41_PENALTY=0.213506
PRIMER_RIGHT_41_PENALTY=1.799414
PRIMER_LEFT_41_SEQUENCE=GTTGCTGGGAAGGCTATGAA
PRIMER_RIGHT_41_SEQUENCE=AAACTTCGCAGCTTATCGTG
PRIMER_LEFT_41=834,20
PRIMER_RIGHT_41=1631,20
PRIMER_LEFT_41_TM=60.214
PRIMER_RIGHT_41_TM=58.201
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=8.4000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=798
PRIMER_PAIR_42_PENALTY=2.067640
PRIMER_LEFT_42_PENALTY=1.035696
PRIMER_RIGHT_42_PENALTY=1.031944
PRIMER_LEFT_42_SEQUENCE=CTTGGGTTGTTAAAGCAGCA
PRIMER_RIGHT_42_SEQUENCE=GAAACTTCGCAGCTTATCGTG
PRIMER_LEFT_42=793,20
PRIMER_RIGHT_42=1632,21
PRIMER_LEFT_42_TM=58.964
PRIMER_RIGHT_42_TM=60.032
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=840
PRIMER_PAIR_43_PENALTY=2.067640
PRIMER_LEFT_43_PENALTY=1.035696
PRIMER_RIGHT_43_PENALTY=1.031944
PRIMER_LEFT_43_SEQUENCE=CTTGGGTTGTTAAAGCAGCA
PRIMER_RIGHT_43_SEQUENCE=TGAAACTTCGCAGCTTATCGT
PRIMER_LEFT_43=793,20
PRIMER_RIGHT_43=1633,21
PRIMER_LEFT_43_TM=58.964
PRIMER_RIGHT_43_TM=60.032
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=8.0000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=841
PRIMER_PAIR_44_PENALTY=2.079288
PRIMER_LEFT_44_PENALTY=1.711575
PRIMER_RIGHT_44_PENALTY=0.367713
PRIMER_LEFT_44_SEQUENCE=TGTTGCTGGGAAGGCTATGA
PRIMER_RIGHT_44_SEQUENCE=CTTCGCAGCTTATCGTGTTG
PRIMER_LEFT_44=833,20
PRIMER_RIGHT_44=1628,20
PRIMER_LEFT_44_TM=61.712
PRIMER_RIGHT_44_TM=59.632
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=5.9000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=7.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=796
PRIMER_PAIR_45_PENALTY=2.106276
PRIMER_LEFT_45_PENALTY=0.163658
PRIMER_RIGHT_45_PENALTY=1.942617
PRIMER_LEFT_45_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_45_SEQUENCE=AACTTCGCAGCTTATCGTGTT
PRIMER_LEFT_45=837,20
PRIMER_RIGHT_45=1630,21
PRIMER_LEFT_45_TM=59.836
PRIMER_RIGHT_45_TM=59.057
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=794
PRIMER_PAIR_46_PENALTY=2.106276
PRIMER_LEFT_46_PENALTY=0.163658
PRIMER_RIGHT_46_PENALTY=1.942617
PRIMER_LEFT_46_SEQUENCE=GCTGGGAAGGCTATGAAGTG
PRIMER_RIGHT_46_SEQUENCE=AAACTTCGCAGCTTATCGTGT
PRIMER_LEFT_46=837,20
PRIMER_RIGHT_46=1631,21
PRIMER_LEFT_46_TM=59.836
PRIMER_RIGHT_46_TM=59.057
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=795
PRIMER_PAIR_47_PENALTY=2.106276
PRIMER_LEFT_47_PENALTY=0.163658
PRIMER_RIGHT_47_PENALTY=1.942617
PRIMER_LEFT_47_SEQUENCE=TGCTGGGAAGGCTATGAAGT
PRIMER_RIGHT_47_SEQUENCE=AACTTCGCAGCTTATCGTGTT
PRIMER_LEFT_47=836,20
PRIMER_RIGHT_47=1630,21
PRIMER_LEFT_47_TM=59.836
PRIMER_RIGHT_47_TM=59.057
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=7.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=795
PRIMER_PAIR_48_PENALTY=2.106276
PRIMER_LEFT_48_PENALTY=0.163658
PRIMER_RIGHT_48_PENALTY=1.942617
PRIMER_LEFT_48_SEQUENCE=TGCTGGGAAGGCTATGAAGT
PRIMER_RIGHT_48_SEQUENCE=AAACTTCGCAGCTTATCGTGT
PRIMER_LEFT_48=836,20
PRIMER_RIGHT_48=1631,21
PRIMER_LEFT_48_TM=59.836
PRIMER_RIGHT_48_TM=59.057
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=7.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=796
PRIMER_PAIR_49_PENALTY=2.131442
PRIMER_LEFT_49_PENALTY=0.332028
PRIMER_RIGHT_49_PENALTY=1.799414
PRIMER_LEFT_49_SEQUENCE=GGGAAATTTGGTGCATTTGT
PRIMER_RIGHT_49_SEQUENCE=AAACTTCGCAGCTTATCGTG
PRIMER_LEFT_49=816,20
PRIMER_RIGHT_49=1631,20
PRIMER_LEFT_49_TM=59.668
PRIMER_RIGHT_49_TM=58.201
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=816
=
SEQUENCE_ID=MAGIC.105_4_126478_126646_zu-0_tsu-0_2879_244446_TRUE
SEQUENCE_TARGET=950,268
SEQUENCE_TEMPLATE=TGGAGAGACTTGAAGNTANCTAGATTCTTAGCCAAAATTCTCGCTGNGAGATAAAGAAAGAATTCTANATTTTGCTTGGCAAAGAGGGNAAGAAGATGANGAAGAAGAAGANACAGNTTTATTTCGTCAACTCTGCTTCTTATTCGGTAACANCGACGTCGTTTTAGTCACCTTCAACTTCTGGGCCTTTTGGGCCTANTGATTCAAATTTGTTGGGCTTNATGCAAGTTTGATTAATTTGGTGCAAAACCTTATTAANAAACACACNCACACAAANNNNNNNNNTTGTGAATTTACTTAACTACGTACATATATATNATCATTAATTAATCACGGTGNTTNTCAAATATATATGGTTACAAATTCAATATGTTTNGGACAAAATAATNTGTGCCTAAATCTGCTTATAAATNACAAAATATATACAACCACTAAAAATAGAAGAGATATTTATCATGAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATTTTGCAAATACTTGGCTACAATNNAAAAAACTGATAGATTTGTGTGNNNANNNNNNTTNTNANTNATTANNTNCNNTAATNNNTAANGNNNNNNTNNNNNNTTNNNTNNTNTNNNNTTNNTNTNNNNNNNANNNNNNNTNNNNNNANNNNNTTNNNNNNNANNAANNNGNNANNTNNNAAANNNNNNTTNNTTNNNANNNNNAANNTTNTTTNNAANNNNANNNNANTNNTNNNNNCNTNANNNNNANNTNNNTTNNNTTNNANTNNANTNNNGTNNNNNNNANNNNNTNTNNNNNNNTNNNNTANNNATNNNNNNNNNNNNANNNACNNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNANNNNNNANNNANTNNANTNNNNNTNANNNNNNANNAANNNNNNTTNNNTNNNNNANNANNANNNNNTCNNNNNCANCANNANCANNACNNTCNCNNNNCNNANNNNNAAANNNNAANNNNTCNTCNTCTATGTCAATCAGAGCCATGNGAGATCAAAAAACACTTTCTTACGACAAATCTAATAAACCCTTTTATTCTTCTTCTTCATCTCCATGTTCCTTCACNGCCATCGTCGTCTTCTTGATCTTCGTCTCATATCTCTTATACTCATTCAGTTTCATNTCTTTTCTAAACCCATATTCACCTTCTAAATCCCCCANTTCTCTCCTCGTACCCGTTATCCGTCTCGGGTCGGGTCAAACACCTGAGGAACAAACGGAGCTCAAACACATAGTCTTCGGAATCGCCGCGTCTTCAGATCTATGGAAGCATCGTAGAGAATACGTGAAAACATGGTGGAAACCTAACGGAGTCATGAACGGCGCCGTTTGGCTCGACAAACCCATCAACGACACCGTTTCTTCTTCATCAGCTTTGCCTCAGATCAGAATCTCTTCCGACACGTCGAGTTTCAAGTACCGTTACCGTAACGGTCACAGATCAGCGATCAGGATCACGAGAATCGTGTCCGAGACGGTGANGATGTTAAACGGAACGGAANCGGAGAGAAACGTGAGGTGGGTTGTGATGGGTGACGACGATACCGTGTTTTTCACGGAAAATCTTGTTAGGGTTTTGAGAAAGTACGATCATAAACAGTTTTATTACATCGGAGCTCCGTCCGAGAGTCATTTGCAGAATCTTCATCAGTTTTCTTATGGAATGGCGTACGGTGGAGGAGGGTTTGCTATAAGTTATCCATTAGCCAANGTTTTGGAGAAGATGCAAGATCGGTGTATCGAGAGATACTCCGATTTGTACGGATCCGATGATCGGATTCATGCTTGTATGGCTGAGCTTGGTGTTCCTCTCACTAAAGAAGTTGGATTCCATCAGGTTAGTTTTGTTATTACCGAGAAAAATCAGCCGGAAAAANACNAAGTAAATGTGAAATAGTAAAACTTAAAATCCGTGATTAGATTCATTTGTCATTGACTCTTGTTACTTCACTTACTNATTATTAATTTTATGGTATGGTTTATAACAGTTTGATGTATACGGGAATCTCTTAGGGCTATTATCTGTCCATCCACAAGCACCAATAGTATCNATCCATCACCTAGACGTAGTGGATCCNATATTCCCAAAAACAAACCGAGTCAACGCATTAAAGAAGCTAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_14749726_14749851_wu-0_mt-0_314425_134545_TRUE
SEQUENCE_TARGET=950,225
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAACGTAGAGTGCAAAAGAAAAAATGTTTGTTTTCATCAAGTTGTATTTTGTTGGTTAAATTCTGGTNAATTNCAGTTATTATAANTGTGGGTTAAATTCCTGTTAACATAGTAAACCAANTTNNTNTGAGATNTAATTGGTTTTGGAACGGTANTGAGAATGNACCAATAAATTGGTTTATNGTTTTATNCATANAAATCNNNNNNNNNNNNNAAGCTCAACTCTCTCCATNATTTTGNCACNTCAGCATTTTAAGCTTTTCTGGAGATGACACATCAGCTTTCTAATCTTNCAANAAAAACATTTCATTAATATGATTCAGCTTTTATTTTTTACACATCAGCTTCTAATTATAAGTGTCAAATANAGTAAACAAAATAGCNCATGTNAGATGNGCCATATNTGTTTTAGAATCTATCNGAGTGCAATANTAATAGGTNGGCCNCATTTCANTGNTGGNTTAATGNTTCTANACCNAANAACATAGTCTCNNTTTCTGTACATCATATTTATTTNNAAAAAANGTTTTATACCTTNTAANTGCCANAATGNANGCCACAGTANAAGNTACACANAACTGAANCAAGGAACAAGCANCCGTAANTGTCTTTTAAATAGATGCACAANTTATTTCACTAAAACATATTCACACTCCCAGCTTATGGGATTACAAGCTTTCACTACNNTAANCTCTNTAAATATTCAAACAGACATTGCTNNNNACGGGACAACATCATAACCAATTTATTTTTATATGTGTTTCAAACAACATCAGTAAATGTTTACCAAGNGACAAGTACCATAAATAGGATACAAAACCATCATCATGTATANCCTATACGGAAATTAAAAAATNAAAACATNANATACNANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNAAATAATGTGNAACGGTCTGATTTGTTNTCTGACTTNAGTTAATTAAAATTAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGATTGTTTCNTTTTTNTTTTTATNTTTACTNAATTCATGTTTTCCTTTTCTTTTCTTTTTTGGTCAAAGTTTTCCTTTNCTTAATTTCNTTTTTCGTCAACAAATTTGTTTATGATTTCCTTTTTTCCAACTGAAGCGTCGAAACGTAAAATATNNNNNNNTNTATATATTAATTCACTCAGCCTCACTTTAAGTCAATTTCATCGATCTCTTCTCTTTGATTTCACAATCTTATCTCTTCATATCAAAAGTTAGGCTTTGAATCTTCNNNTTCTTCTTCTTCTTCCACCATGAATACATATAGTAATTTGGTGTCCGTNAAGATTACTACAACAGCAATGATCCTACCACAAGATCAAGGGTTTAGAAACACCGTGTTCGTNAATCTACANGATGTGGTCAACGAGTTAGCTGAAGATCAAAACNGTGTCNTANTGTGTTTGGGAACGTTCGCGGCTCCACTTATGACTAAAATCGAATTGAAACCATCATNTCTCAGTTCACATCACATCTATCAAGACCTTGACNAGTTCATCACACATACTNAGTTTTGTCNNNTGTTGAGCGAAAGGATCGNTTTNGATTGTGCTCATATTCCCCAACCTTTTANNGTNTCCTTNTACGTNGAAGTGACAAGAGATGTTATGTTCTCGTCTATTGCTGTTAGGTCGANAGATACGTTTCANAGATTGCTTGAGGNNNNAACAATNGAATTGACTGATTTGGGAGATGANGAAGAAACCACTTGTTCGATTTGTTTGGAGGACTTTTCGGAGAGTCATGATGATAACATCAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.819406
PRIMER_LEFT_0_PENALTY=0.753052
PRIMER_RIGHT_0_PENALTY=1.066354
PRIMER_LEFT_0_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_0_SEQUENCE=TTACGTTTCGACGCTTCAGTT
PRIMER_LEFT_0=828,20
PRIMER_RIGHT_0=1477,21
PRIMER_LEFT_0_TM=59.247
PRIMER_RIGHT_0_TM=59.934
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=650
PRIMER_PAIR_1_PENALTY=1.819406
PRIMER_LEFT_1_PENALTY=0.753052
PRIMER_RIGHT_1_PENALTY=1.066354
PRIMER_LEFT_1_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_1_SEQUENCE=TTTACGTTTCGACGCTTCAGT
PRIMER_LEFT_1=828,20
PRIMER_RIGHT_1=1478,21
PRIMER_LEFT_1_TM=59.247
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=651
PRIMER_PAIR_2_PENALTY=2.052007
PRIMER_LEFT_2_PENALTY=0.753052
PRIMER_RIGHT_2_PENALTY=1.298955
PRIMER_LEFT_2_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_2_SEQUENCE=CGACGCTTCAGTTGGAAAAA
PRIMER_LEFT_2=828,20
PRIMER_RIGHT_2=1469,20
PRIMER_LEFT_2_TM=59.247
PRIMER_RIGHT_2_TM=61.299
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=642
PRIMER_PAIR_3_PENALTY=2.169459
PRIMER_LEFT_3_PENALTY=1.103105
PRIMER_RIGHT_3_PENALTY=1.066354
PRIMER_LEFT_3_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_3_SEQUENCE=TTACGTTTCGACGCTTCAGTT
PRIMER_LEFT_3=827,21
PRIMER_RIGHT_3=1477,21
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=651
PRIMER_PAIR_4_PENALTY=2.169459
PRIMER_LEFT_4_PENALTY=1.103105
PRIMER_RIGHT_4_PENALTY=1.066354
PRIMER_LEFT_4_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_4_SEQUENCE=TTTACGTTTCGACGCTTCAGT
PRIMER_LEFT_4=827,21
PRIMER_RIGHT_4=1478,21
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=652
PRIMER_PAIR_5_PENALTY=2.172972
PRIMER_LEFT_5_PENALTY=0.753052
PRIMER_RIGHT_5_PENALTY=1.419920
PRIMER_LEFT_5_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_5_SEQUENCE=TACGTTTCGACGCTTCAGTT
PRIMER_LEFT_5=828,20
PRIMER_RIGHT_5=1476,20
PRIMER_LEFT_5_TM=59.247
PRIMER_RIGHT_5_TM=58.580
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=649
PRIMER_PAIR_6_PENALTY=2.172972
PRIMER_LEFT_6_PENALTY=0.753052
PRIMER_RIGHT_6_PENALTY=1.419920
PRIMER_LEFT_6_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_6_SEQUENCE=TTACGTTTCGACGCTTCAGT
PRIMER_LEFT_6=828,20
PRIMER_RIGHT_6=1477,20
PRIMER_LEFT_6_TM=59.247
PRIMER_RIGHT_6_TM=58.580
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=650
PRIMER_PAIR_7_PENALTY=2.253212
PRIMER_LEFT_7_PENALTY=0.753052
PRIMER_RIGHT_7_PENALTY=1.500160
PRIMER_LEFT_7_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_7_SEQUENCE=TCAGCTAACTCGTTGACCACA
PRIMER_LEFT_7=828,20
PRIMER_RIGHT_7=1743,21
PRIMER_LEFT_7_TM=59.247
PRIMER_RIGHT_7_TM=59.500
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=916
PRIMER_PAIR_8_PENALTY=2.344520
PRIMER_LEFT_8_PENALTY=1.278166
PRIMER_RIGHT_8_PENALTY=1.066354
PRIMER_LEFT_8_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_8_SEQUENCE=TTACGTTTCGACGCTTCAGTT
PRIMER_LEFT_8=750,20
PRIMER_RIGHT_8=1477,21
PRIMER_LEFT_8_TM=58.722
PRIMER_RIGHT_8_TM=59.934
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=728
PRIMER_PAIR_9_PENALTY=2.344520
PRIMER_LEFT_9_PENALTY=1.278166
PRIMER_RIGHT_9_PENALTY=1.066354
PRIMER_LEFT_9_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_9_SEQUENCE=TTTACGTTTCGACGCTTCAGT
PRIMER_LEFT_9=750,20
PRIMER_RIGHT_9=1478,21
PRIMER_LEFT_9_TM=58.722
PRIMER_RIGHT_9_TM=59.934
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=729
PRIMER_PAIR_10_PENALTY=2.369304
PRIMER_LEFT_10_PENALTY=1.302950
PRIMER_RIGHT_10_PENALTY=1.066354
PRIMER_LEFT_10_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_10_SEQUENCE=TTACGTTTCGACGCTTCAGTT
PRIMER_LEFT_10=827,20
PRIMER_RIGHT_10=1477,21
PRIMER_LEFT_10_TM=58.697
PRIMER_RIGHT_10_TM=59.934
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=651
PRIMER_PAIR_11_PENALTY=2.369304
PRIMER_LEFT_11_PENALTY=1.302950
PRIMER_RIGHT_11_PENALTY=1.066354
PRIMER_LEFT_11_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_11_SEQUENCE=TTTACGTTTCGACGCTTCAGT
PRIMER_LEFT_11=827,20
PRIMER_RIGHT_11=1478,21
PRIMER_LEFT_11_TM=58.697
PRIMER_RIGHT_11_TM=59.934
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=652
PRIMER_PAIR_12_PENALTY=2.402059
PRIMER_LEFT_12_PENALTY=1.103105
PRIMER_RIGHT_12_PENALTY=1.298955
PRIMER_LEFT_12_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_12_SEQUENCE=CGACGCTTCAGTTGGAAAAA
PRIMER_LEFT_12=827,21
PRIMER_RIGHT_12=1469,20
PRIMER_LEFT_12_TM=60.103
PRIMER_RIGHT_12_TM=61.299
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=643
PRIMER_PAIR_13_PENALTY=2.523024
PRIMER_LEFT_13_PENALTY=1.103105
PRIMER_RIGHT_13_PENALTY=1.419920
PRIMER_LEFT_13_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_13_SEQUENCE=TACGTTTCGACGCTTCAGTT
PRIMER_LEFT_13=827,21
PRIMER_RIGHT_13=1476,20
PRIMER_LEFT_13_TM=60.103
PRIMER_RIGHT_13_TM=58.580
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=650
PRIMER_PAIR_14_PENALTY=2.523024
PRIMER_LEFT_14_PENALTY=1.103105
PRIMER_RIGHT_14_PENALTY=1.419920
PRIMER_LEFT_14_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_14_SEQUENCE=TTACGTTTCGACGCTTCAGT
PRIMER_LEFT_14=827,21
PRIMER_RIGHT_14=1477,20
PRIMER_LEFT_14_TM=60.103
PRIMER_RIGHT_14_TM=58.580
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=651
PRIMER_PAIR_15_PENALTY=2.577120
PRIMER_LEFT_15_PENALTY=1.278166
PRIMER_RIGHT_15_PENALTY=1.298955
PRIMER_LEFT_15_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_15_SEQUENCE=CGACGCTTCAGTTGGAAAAA
PRIMER_LEFT_15=750,20
PRIMER_RIGHT_15=1469,20
PRIMER_LEFT_15_TM=58.722
PRIMER_RIGHT_15_TM=61.299
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.4000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=720
PRIMER_PAIR_16_PENALTY=2.601905
PRIMER_LEFT_16_PENALTY=1.302950
PRIMER_RIGHT_16_PENALTY=1.298955
PRIMER_LEFT_16_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_16_SEQUENCE=CGACGCTTCAGTTGGAAAAA
PRIMER_LEFT_16=827,20
PRIMER_RIGHT_16=1469,20
PRIMER_LEFT_16_TM=58.697
PRIMER_RIGHT_16_TM=61.299
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=643
PRIMER_PAIR_17_PENALTY=2.603265
PRIMER_LEFT_17_PENALTY=1.103105
PRIMER_RIGHT_17_PENALTY=1.500160
PRIMER_LEFT_17_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_17_SEQUENCE=TCAGCTAACTCGTTGACCACA
PRIMER_LEFT_17=827,21
PRIMER_RIGHT_17=1743,21
PRIMER_LEFT_17_TM=60.103
PRIMER_RIGHT_17_TM=59.500
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=917
PRIMER_PAIR_18_PENALTY=2.604233
PRIMER_LEFT_18_PENALTY=0.753052
PRIMER_RIGHT_18_PENALTY=1.851181
PRIMER_LEFT_18_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_18_SEQUENCE=TCGACGCTTCAGTTGGAAAA
PRIMER_LEFT_18=828,20
PRIMER_RIGHT_18=1470,20
PRIMER_LEFT_18_TM=59.247
PRIMER_RIGHT_18_TM=61.851
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=643
PRIMER_PAIR_19_PENALTY=2.604233
PRIMER_LEFT_19_PENALTY=0.753052
PRIMER_RIGHT_19_PENALTY=1.851181
PRIMER_LEFT_19_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_19_SEQUENCE=TTCGACGCTTCAGTTGGAAA
PRIMER_LEFT_19=828,20
PRIMER_RIGHT_19=1471,20
PRIMER_LEFT_19_TM=59.247
PRIMER_RIGHT_19_TM=61.851
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=644
PRIMER_PAIR_20_PENALTY=2.698086
PRIMER_LEFT_20_PENALTY=1.278166
PRIMER_RIGHT_20_PENALTY=1.419920
PRIMER_LEFT_20_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_20_SEQUENCE=TACGTTTCGACGCTTCAGTT
PRIMER_LEFT_20=750,20
PRIMER_RIGHT_20=1476,20
PRIMER_LEFT_20_TM=58.722
PRIMER_RIGHT_20_TM=58.580
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.4000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=727
PRIMER_PAIR_21_PENALTY=2.698086
PRIMER_LEFT_21_PENALTY=1.278166
PRIMER_RIGHT_21_PENALTY=1.419920
PRIMER_LEFT_21_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_21_SEQUENCE=TTACGTTTCGACGCTTCAGT
PRIMER_LEFT_21=750,20
PRIMER_RIGHT_21=1477,20
PRIMER_LEFT_21_TM=58.722
PRIMER_RIGHT_21_TM=58.580
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.4000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=728
PRIMER_PAIR_22_PENALTY=2.722870
PRIMER_LEFT_22_PENALTY=1.302950
PRIMER_RIGHT_22_PENALTY=1.419920
PRIMER_LEFT_22_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_22_SEQUENCE=TACGTTTCGACGCTTCAGTT
PRIMER_LEFT_22=827,20
PRIMER_RIGHT_22=1476,20
PRIMER_LEFT_22_TM=58.697
PRIMER_RIGHT_22_TM=58.580
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=650
PRIMER_PAIR_23_PENALTY=2.722870
PRIMER_LEFT_23_PENALTY=1.302950
PRIMER_RIGHT_23_PENALTY=1.419920
PRIMER_LEFT_23_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_23_SEQUENCE=TTACGTTTCGACGCTTCAGT
PRIMER_LEFT_23=827,20
PRIMER_RIGHT_23=1477,20
PRIMER_LEFT_23_TM=58.697
PRIMER_RIGHT_23_TM=58.580
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=651
PRIMER_PAIR_24_PENALTY=2.778326
PRIMER_LEFT_24_PENALTY=1.278166
PRIMER_RIGHT_24_PENALTY=1.500160
PRIMER_LEFT_24_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_24_SEQUENCE=TCAGCTAACTCGTTGACCACA
PRIMER_LEFT_24=750,20
PRIMER_RIGHT_24=1743,21
PRIMER_LEFT_24_TM=58.722
PRIMER_RIGHT_24_TM=59.500
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=994
PRIMER_PAIR_25_PENALTY=2.803110
PRIMER_LEFT_25_PENALTY=1.302950
PRIMER_RIGHT_25_PENALTY=1.500160
PRIMER_LEFT_25_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_25_SEQUENCE=TCAGCTAACTCGTTGACCACA
PRIMER_LEFT_25=827,20
PRIMER_RIGHT_25=1743,21
PRIMER_LEFT_25_TM=58.697
PRIMER_RIGHT_25_TM=59.500
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=917
PRIMER_PAIR_26_PENALTY=2.885314
PRIMER_LEFT_26_PENALTY=0.753052
PRIMER_RIGHT_26_PENALTY=2.132262
PRIMER_LEFT_26_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_26_SEQUENCE=TCTTGTGGTAGGATCATTGCTG
PRIMER_LEFT_26=828,20
PRIMER_RIGHT_26=1683,22
PRIMER_LEFT_26_TM=59.247
PRIMER_RIGHT_26_TM=60.132
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=856
PRIMER_PAIR_27_PENALTY=2.950192
PRIMER_LEFT_27_PENALTY=0.753052
PRIMER_RIGHT_27_PENALTY=2.197140
PRIMER_LEFT_27_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_27_SEQUENCE=CAGCTAACTCGTTGACCACATC
PRIMER_LEFT_27=828,20
PRIMER_RIGHT_27=1742,22
PRIMER_LEFT_27_TM=59.247
PRIMER_RIGHT_27_TM=59.803
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.3000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=915
PRIMER_PAIR_28_PENALTY=2.954286
PRIMER_LEFT_28_PENALTY=1.103105
PRIMER_RIGHT_28_PENALTY=1.851181
PRIMER_LEFT_28_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_28_SEQUENCE=TCGACGCTTCAGTTGGAAAA
PRIMER_LEFT_28=827,21
PRIMER_RIGHT_28=1470,20
PRIMER_LEFT_28_TM=60.103
PRIMER_RIGHT_28_TM=61.851
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=644
PRIMER_PAIR_29_PENALTY=2.954286
PRIMER_LEFT_29_PENALTY=1.103105
PRIMER_RIGHT_29_PENALTY=1.851181
PRIMER_LEFT_29_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_29_SEQUENCE=TTCGACGCTTCAGTTGGAAA
PRIMER_LEFT_29=827,21
PRIMER_RIGHT_29=1471,20
PRIMER_LEFT_29_TM=60.103
PRIMER_RIGHT_29_TM=61.851
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=645
PRIMER_PAIR_30_PENALTY=3.000231
PRIMER_LEFT_30_PENALTY=1.933877
PRIMER_RIGHT_30_PENALTY=1.066354
PRIMER_LEFT_30_SEQUENCE=ATTCACACTCCCAGCTTATGG
PRIMER_RIGHT_30_SEQUENCE=TTACGTTTCGACGCTTCAGTT
PRIMER_LEFT_30=749,21
PRIMER_RIGHT_30=1477,21
PRIMER_LEFT_30_TM=59.066
PRIMER_RIGHT_30_TM=59.934
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.4000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=729
PRIMER_PAIR_31_PENALTY=3.000231
PRIMER_LEFT_31_PENALTY=1.933877
PRIMER_RIGHT_31_PENALTY=1.066354
PRIMER_LEFT_31_SEQUENCE=ATTCACACTCCCAGCTTATGG
PRIMER_RIGHT_31_SEQUENCE=TTTACGTTTCGACGCTTCAGT
PRIMER_LEFT_31=749,21
PRIMER_RIGHT_31=1478,21
PRIMER_LEFT_31_TM=59.066
PRIMER_RIGHT_31_TM=59.934
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.4000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=730
PRIMER_PAIR_32_PENALTY=3.022705
PRIMER_LEFT_32_PENALTY=0.753052
PRIMER_RIGHT_32_PENALTY=2.269653
PRIMER_LEFT_32_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_32_SEQUENCE=CGGTGTTTCTAAACCCTTGA
PRIMER_LEFT_32=828,20
PRIMER_RIGHT_32=1703,20
PRIMER_LEFT_32_TM=59.247
PRIMER_RIGHT_32_TM=57.730
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=876
PRIMER_PAIR_33_PENALTY=3.129347
PRIMER_LEFT_33_PENALTY=1.278166
PRIMER_RIGHT_33_PENALTY=1.851181
PRIMER_LEFT_33_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_33_SEQUENCE=TCGACGCTTCAGTTGGAAAA
PRIMER_LEFT_33=750,20
PRIMER_RIGHT_33=1470,20
PRIMER_LEFT_33_TM=58.722
PRIMER_RIGHT_33_TM=61.851
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.4000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=721
PRIMER_PAIR_34_PENALTY=3.129347
PRIMER_LEFT_34_PENALTY=1.278166
PRIMER_RIGHT_34_PENALTY=1.851181
PRIMER_LEFT_34_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_34_SEQUENCE=TTCGACGCTTCAGTTGGAAA
PRIMER_LEFT_34=750,20
PRIMER_RIGHT_34=1471,20
PRIMER_LEFT_34_TM=58.722
PRIMER_RIGHT_34_TM=61.851
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.4000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=722
PRIMER_PAIR_35_PENALTY=3.154131
PRIMER_LEFT_35_PENALTY=1.302950
PRIMER_RIGHT_35_PENALTY=1.851181
PRIMER_LEFT_35_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_35_SEQUENCE=TCGACGCTTCAGTTGGAAAA
PRIMER_LEFT_35=827,20
PRIMER_RIGHT_35=1470,20
PRIMER_LEFT_35_TM=58.697
PRIMER_RIGHT_35_TM=61.851
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.3000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=644
PRIMER_PAIR_36_PENALTY=3.154131
PRIMER_LEFT_36_PENALTY=1.302950
PRIMER_RIGHT_36_PENALTY=1.851181
PRIMER_LEFT_36_SEQUENCE=ACGGGACAACATCATAACCA
PRIMER_RIGHT_36_SEQUENCE=TTCGACGCTTCAGTTGGAAA
PRIMER_LEFT_36=827,20
PRIMER_RIGHT_36=1471,20
PRIMER_LEFT_36_TM=58.697
PRIMER_RIGHT_36_TM=61.851
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=645
PRIMER_PAIR_37_PENALTY=3.209909
PRIMER_LEFT_37_PENALTY=2.143555
PRIMER_RIGHT_37_PENALTY=1.066354
PRIMER_LEFT_37_SEQUENCE=CACTCCCAGCTTATGGGATTAC
PRIMER_RIGHT_37_SEQUENCE=TTACGTTTCGACGCTTCAGTT
PRIMER_LEFT_37=754,22
PRIMER_RIGHT_37=1477,21
PRIMER_LEFT_37_TM=59.856
PRIMER_RIGHT_37_TM=59.934
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=5.6000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=724
PRIMER_PAIR_38_PENALTY=3.209909
PRIMER_LEFT_38_PENALTY=2.143555
PRIMER_RIGHT_38_PENALTY=1.066354
PRIMER_LEFT_38_SEQUENCE=CACTCCCAGCTTATGGGATTAC
PRIMER_RIGHT_38_SEQUENCE=TTTACGTTTCGACGCTTCAGT
PRIMER_LEFT_38=754,22
PRIMER_RIGHT_38=1478,21
PRIMER_LEFT_38_TM=59.856
PRIMER_RIGHT_38_TM=59.934
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=5.6000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=725
PRIMER_PAIR_39_PENALTY=3.232832
PRIMER_LEFT_39_PENALTY=1.933877
PRIMER_RIGHT_39_PENALTY=1.298955
PRIMER_LEFT_39_SEQUENCE=ATTCACACTCCCAGCTTATGG
PRIMER_RIGHT_39_SEQUENCE=CGACGCTTCAGTTGGAAAAA
PRIMER_LEFT_39=749,21
PRIMER_RIGHT_39=1469,20
PRIMER_LEFT_39_TM=59.066
PRIMER_RIGHT_39_TM=61.299
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.4000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=721
PRIMER_PAIR_40_PENALTY=3.235367
PRIMER_LEFT_40_PENALTY=1.103105
PRIMER_RIGHT_40_PENALTY=2.132262
PRIMER_LEFT_40_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_40_SEQUENCE=TCTTGTGGTAGGATCATTGCTG
PRIMER_LEFT_40=827,21
PRIMER_RIGHT_40=1683,22
PRIMER_LEFT_40_TM=60.103
PRIMER_RIGHT_40_TM=60.132
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=857
PRIMER_PAIR_41_PENALTY=3.281363
PRIMER_LEFT_41_PENALTY=0.753052
PRIMER_RIGHT_41_PENALTY=2.528311
PRIMER_LEFT_41_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_41_SEQUENCE=CAGCTAACTCGTTGACCACA
PRIMER_LEFT_41=828,20
PRIMER_RIGHT_41=1742,20
PRIMER_LEFT_41_TM=59.247
PRIMER_RIGHT_41_TM=57.472
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=915
PRIMER_PAIR_42_PENALTY=3.300245
PRIMER_LEFT_42_PENALTY=1.103105
PRIMER_RIGHT_42_PENALTY=2.197140
PRIMER_LEFT_42_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_42_SEQUENCE=CAGCTAACTCGTTGACCACATC
PRIMER_LEFT_42=827,21
PRIMER_RIGHT_42=1742,22
PRIMER_LEFT_42_TM=60.103
PRIMER_RIGHT_42_TM=59.803
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=916
PRIMER_PAIR_43_PENALTY=3.353797
PRIMER_LEFT_43_PENALTY=1.933877
PRIMER_RIGHT_43_PENALTY=1.419920
PRIMER_LEFT_43_SEQUENCE=ATTCACACTCCCAGCTTATGG
PRIMER_RIGHT_43_SEQUENCE=TACGTTTCGACGCTTCAGTT
PRIMER_LEFT_43=749,21
PRIMER_RIGHT_43=1476,20
PRIMER_LEFT_43_TM=59.066
PRIMER_RIGHT_43_TM=58.580
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.4000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=728
PRIMER_PAIR_44_PENALTY=3.353797
PRIMER_LEFT_44_PENALTY=1.933877
PRIMER_RIGHT_44_PENALTY=1.419920
PRIMER_LEFT_44_SEQUENCE=ATTCACACTCCCAGCTTATGG
PRIMER_RIGHT_44_SEQUENCE=TTACGTTTCGACGCTTCAGT
PRIMER_LEFT_44=749,21
PRIMER_RIGHT_44=1477,20
PRIMER_LEFT_44_TM=59.066
PRIMER_RIGHT_44_TM=58.580
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.4000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=729
PRIMER_PAIR_45_PENALTY=3.364262
PRIMER_LEFT_45_PENALTY=0.753052
PRIMER_RIGHT_45_PENALTY=2.611210
PRIMER_LEFT_45_SEQUENCE=CGGGACAACATCATAACCAA
PRIMER_RIGHT_45_SEQUENCE=AAACCCTTGATCTTGTGGTAGG
PRIMER_LEFT_45=828,20
PRIMER_RIGHT_45=1693,22
PRIMER_LEFT_45_TM=59.247
PRIMER_RIGHT_45_TM=59.389
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=866
PRIMER_PAIR_46_PENALTY=3.372757
PRIMER_LEFT_46_PENALTY=1.103105
PRIMER_RIGHT_46_PENALTY=2.269653
PRIMER_LEFT_46_SEQUENCE=ACGGGACAACATCATAACCAA
PRIMER_RIGHT_46_SEQUENCE=CGGTGTTTCTAAACCCTTGA
PRIMER_LEFT_46=827,21
PRIMER_RIGHT_46=1703,20
PRIMER_LEFT_46_TM=60.103
PRIMER_RIGHT_46_TM=57.730
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=877
PRIMER_PAIR_47_PENALTY=3.390709
PRIMER_LEFT_47_PENALTY=2.324355
PRIMER_RIGHT_47_PENALTY=1.066354
PRIMER_LEFT_47_SEQUENCE=TCCCAGCTTATGGGATTACAAG
PRIMER_RIGHT_47_SEQUENCE=TTACGTTTCGACGCTTCAGTT
PRIMER_LEFT_47=757,22
PRIMER_RIGHT_47=1477,21
PRIMER_LEFT_47_TM=60.324
PRIMER_RIGHT_47_TM=59.934
PRIMER_LEFT_47_GC_PERCENT=45.455
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=721
PRIMER_PAIR_48_PENALTY=3.390709
PRIMER_LEFT_48_PENALTY=2.324355
PRIMER_RIGHT_48_PENALTY=1.066354
PRIMER_LEFT_48_SEQUENCE=TCCCAGCTTATGGGATTACAAG
PRIMER_RIGHT_48_SEQUENCE=TTTACGTTTCGACGCTTCAGT
PRIMER_LEFT_48=757,22
PRIMER_RIGHT_48=1478,21
PRIMER_LEFT_48_TM=60.324
PRIMER_RIGHT_48_TM=59.934
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=722
PRIMER_PAIR_49_PENALTY=3.410428
PRIMER_LEFT_49_PENALTY=1.278166
PRIMER_RIGHT_49_PENALTY=2.132262
PRIMER_LEFT_49_SEQUENCE=TTCACACTCCCAGCTTATGG
PRIMER_RIGHT_49_SEQUENCE=TCTTGTGGTAGGATCATTGCTG
PRIMER_LEFT_49=750,20
PRIMER_RIGHT_49=1683,22
PRIMER_LEFT_49_TM=58.722
PRIMER_RIGHT_49_TM=60.132
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.4000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=934
=
SEQUENCE_ID=MAGIC.446_4_4722510_4722533_wu-0_bur-0_9861_7607_TRUE
SEQUENCE_TARGET=950,123
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTNTACNGGTTNATAACNGATTTANAAANTAATNNNNNNNNNNNNNNNNNNNNNNNNTNTAANTNTTGAATTAAATNTATGTNATCAGNTTATNAATATANNTNGTTANTTNGCANNGNANAGATNAACTNAAATAAACCNANNTTNNNAAGCANTCCAANNGTNGNGNCTCTANTNNTANNNACACTCANACANACTGGANCNAACNGNATGCTAGNGNNGNNNAGANNNNNNNNNNNNNNNNNNNNGACAANNTNTTTTGTCTNTTATGNGTTTAGGNTTTNGGNGNNNNNNNNNNNNNNNNNNNNATTAANCGTACAACATNATTATATANATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCCNAATTACNAAACTCTCNACAANNTTNATTGTTTAATAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANGTNATNNATTATAAGANGNTTNTANCAAAACATTATAATCANCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNATANAANTTAAACTAGAGTNAGTACAAATGATTTNANGANATTCCCAANNANGTNCCNCTTGTANNNGAATCATCTATTCNNNTAATCCTTTNNNNNNNNNNTCTNGATCTTAGCATAAGGTAAGAAANNGTGTCAACCTTATTANACTNAATCATATTNATCNAACTCTGANTTNNNNTGANAAANAAACGATTGACTNTCAATCNNTTTNAGCACGGNCATGCATTTCTCNNTATCAANTNNTCGATAGGCCCAAAGATATTTANCTCCTNTTATATAGGAGGGANAAATCCCATNTTGTTCCATCCNTGTTCCTTATANAATTCATNATACNNCCAATNANTNTCTTTANAATCANCTGTTACNGNTGANGTTTGANANAGTCNANACNNACCAATCCATGNATAANANAACATGACNAACTNAGGTCAAAGGACTANACTCTATAGTNGNGANNNGAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14366390_14366695_kn-0_tsu-0_26304_14412_TRUE
SEQUENCE_TARGET=950,405
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_15360038_15360582_po-0_hi-0_6273_271123_TRUE
SEQUENCE_TARGET=950,644
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_5_12743567_12743916_edi-0_wil-2_12321_9032_TRUE
SEQUENCE_TARGET=950,449
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14329627_14329819_ws-0_hi-0_291214_10612_TRUE
SEQUENCE_TARGET=950,292
SEQUENCE_TEMPLATE=GNAAGAATNGTTTGACATGTTTTTNCTTTTGCTAAATGCTTTCTCTCGTTGTTCACTTTCATTTGGCCTCTTGAACCATTTATATGCGACNTCGAGCAGTTCATGTTTGACTTTGNGAGTTTTTTATACCAAAATAATATTTTTANACTAACAAAGCCNTTTTAGTCGACGTCATAGCTTCTTCCTAGACGCAAAATCTTTTNNAGGTGATTACTTTAGAATGGTNTAGTNTAATCCGNTGGTTTTNTCGGNNAATTAGANATTTAATTGNTATGGAAANNNNTAATGGCANATATANTCATGTATTTATGTGATTGNGAAGACAATAAAATAANTTTCANTANATATATATNCTANAATCAATTACTAATNNCAACAAATTTGNGAGGAAATTNGATTNAGTANGAAAANTTTGANAGATATATAACTTTNCATTTNNANNNANNNTNANNTGTTGGATCAAATTTTNTTAAGTCAANTGGGNTTNATAAAATTNAATTGTGTGAATGAGAANTGGNNNNNNNNNNNNNNGTTTGTTCTCACNGNAGCTCCANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANAANNNNNNTCNTTNGTTTCACGTNTCCTTGAGACTCGGNTTCNTTGTGAGATCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTTGTGGTTGATNGATCTTNAAAATNAAACCAATCTCAAACCNCNNTATATGGACGTTTGAGGTCTTTTTCTTAAGGGACTGAGAATTTCATATCGCTCATATAGAAACACATATNTCCAGCAATTTCTCTTTGNTAGAAAGTNNTNAANAGTNTCGTNNNNNNANTTGGTTCGTTAGAGCTGNTNNCCGNNNNNAGTTTATANAGGTTGTATCNTGNNATCCTTGAATCACACANAAACCGTCANACNANGGNTGATTCAAAGGGTTAAGGANAGAGTTCATNAACTCGACTNCTNTCTTNTCTTTNNGTANCATTTNTGNTTTNNATTNNAANNNNTTTACGTAGTGNTTNNTAATTTTAGCTCCTACNGNNTNAANCCTNTNNNNNNGNTTTGTTCTANAAACATNAACNGATTGNAATTNNANNTTNATAAACGTGGGNAGNNNGTGAGTGTGTACATTGAGTTTANTGGTTGCGANTGGTAAACGTCNAANNNNTTNTGNCNANTNGCGAGNNTNGACTTNGTTNTTCTTTNGGAAACAGAGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_19372776_19372959_wu-0_ler-0_337907_734124_TRUE
SEQUENCE_TARGET=950,283
SEQUENCE_TEMPLATE=AAAGAGCTGATTAAATTNGCAGATGCGCTTACGAAATCTCGTTCNACAAAGAAACTGCATAAGAAATCTGTGTCCTTCTTTTTCTGAAAGTAAGTAGAAACACTAACTTACTTGTTACNGTAACACGCATNGCCTAAAGCNACATGTATGTGTATATAGATNTAGCTTAAAGTTTGTAAATTTGTGAANCAAAACATAAGAAAAGNGTGGAGAGAATCTGATATGAAGCTTGAGAGAAATGAGTAACGCTTGNTGAGGAGCATTGAAACTTAATTTATGACATGTTATTGTNGATGTNGAAAGNCANTCGCGTTTATNCAAACATAGGAGNCATGATACATTACAGAGTTGAACAGAGTTTGGGANNNNNNNNNNNNNNNAGATATGAAAAATGATTAGTTGGTNATGGGNAGGACATGGACNCCAATTTNATGAAGCATGTTGTTGGNTTTCCATGAAACCCTACNATTTTATGACCTTCCTTGTGGAGTACGAAATTTGAAANCATATCGAGTCCAAAGGCTTNAGAGGNTCGNTTGTTGGTCTTGAANTTAAGCATNANTAAAACTTNANNTTCANTTCCATCNANTTTATCATANCTACCCTCCANTGATATCAAGTATTCACTNGGATACTCCANCTCGAACTGNCAAANCNNNNNNAAACANCNTGATCACTTCAAAAAATAATTGGCTAANNTTTAGGTTAATACNTACATTAACGTATAACAGTTACTTGAATCGGAACAACAATNGTTAGTATCTANTCAACGAGNNAAAATCAAANATAACANTANAAAACAAANGAATGNTTTATTAAATAGTTTANATCTAAATATTAGCGTACNATTGTAACNGTTTCTTCTGGANCANAATTAGCAACAAATATNTNNTGTATGCTAAAATTTAAGTTNTCGAATAAATNTTATTTATTGATCAAATCCANNCAAAANGACATNGTTATTAGAAAGTTGAATTCATGTGTTCTGAATCAGAGTTAGNATCTACTAATGTGGTTATGTTAATACGTAAATTAACGAATAACAGTTCNTTGAATCGGAACAAAAATAGTTAGCATCNATCCGAAANAGAAATTCAAAANGAACANTATTAAAAAATAAATGGNTTATTAAAATGGTTAGCATCTAAATGTTAGNGNACAANGGTAATAGNTTCTTCGNNANNAGGGTTAGCAANAAANATTTANTATATGCTAATATTTAACTNAACGTATAACANATGTTGGATCNAANNNAAAAAANAANCNAAAAATATTATNTTGCCGTCTGACACAATATAGNGNCACNNAGGTNATTATACTNAGCANNTAACAGTTGNNNNNNNGNANACTTAATTTTGGAGTCTTCNNNNNNNNGACNATGATNANNNCNTNTNTTTTTGTCACAAGTGTGTTTTNTTTAGTNTNAAAAAAAATAACATGACTATATTTTACAAACATTAAAACAAGTAAACCTAACAAAACAAAACAAAACCAAANAAAAAAAACTCTAAACTATAGGANAAGCTAACACAACATATTAGACACGTNGNAAGCNGGAAACANGTTAGNNGACANGTGCAAANTTNAAGATGTNNGAGTNTNNGTTGATCNGAAAGCTTCAATTNCGGCCAAGATCGCCGGAAAATTATTCAATCNACTTATCTNAATCTTCNCTCAAAGAAACAAANCGNAATTCNATTTTAGCTNTGATGAGATTATGACTCCACAANNNAAATTTGAAAATCTTCTTCATCTTCTTNTTCNATCAAGCNCTTTTCAATCTTNAANGAAATTATCAAATATAGGNTTNCTTTCATTCAACNCTGATNTTAGGNAAAGAAGATNAGCCTAAAACCATGATNCTGANATANACTGCACAATTTATTCATGAGAAAGACAANAAGNGGCAACANATANCCGNAANATNAGCCGNANCANACCGNAAACAGAGCCAGACCACACCNNAAACAAAGTCNNTTCTNATNNGGACAACGAAAGCCNNTAAGCATACCNAANGGTGGAACAAGGATTGAGTTTTCTCTCTCTNAANAATCNTANANAGAGAGANCTCANNCTTTGTGACCAGNCTANTCCAAACACTTACACCNAGAGAGACTTGAAATTCTATTTCTTTTTTGGATATCATTCTCTCAATANTGAATACTACAACAATGTNATTTCAT
PRIMER_LEFT_NUM_RETURNED=6
PRIMER_RIGHT_NUM_RETURNED=6
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=6
PRIMER_PAIR_0_PENALTY=6.508104
PRIMER_LEFT_0_PENALTY=5.542824
PRIMER_RIGHT_0_PENALTY=0.965280
PRIMER_LEFT_0_SEQUENCE=CAGTTACTTGAATCGGAACAAC
PRIMER_RIGHT_0_SEQUENCE=ATAATTTTCCGGCGATCTTG
PRIMER_LEFT_0=728,22
PRIMER_RIGHT_0=1648,20
PRIMER_LEFT_0_TM=56.457
PRIMER_RIGHT_0_TM=59.035
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=921
PRIMER_PAIR_1_PENALTY=7.664973
PRIMER_LEFT_1_PENALTY=5.542824
PRIMER_RIGHT_1_PENALTY=2.122149
PRIMER_LEFT_1_SEQUENCE=CAGTTACTTGAATCGGAACAAC
PRIMER_RIGHT_1_SEQUENCE=TAATTTTCCGGCGATCTTGG
PRIMER_LEFT_1=728,22
PRIMER_RIGHT_1=1647,20
PRIMER_LEFT_1_TM=56.457
PRIMER_RIGHT_1_TM=62.122
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=920
PRIMER_PAIR_2_PENALTY=8.808968
PRIMER_LEFT_2_PENALTY=5.542824
PRIMER_RIGHT_2_PENALTY=3.266144
PRIMER_LEFT_2_SEQUENCE=CAGTTACTTGAATCGGAACAAC
PRIMER_RIGHT_2_SEQUENCE=ATAATTTTCCGGCGATCTTGG
PRIMER_LEFT_2=728,22
PRIMER_RIGHT_2=1648,21
PRIMER_LEFT_2_TM=56.457
PRIMER_RIGHT_2_TM=62.266
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=921
PRIMER_PAIR_3_PENALTY=9.428180
PRIMER_LEFT_3_PENALTY=5.542824
PRIMER_RIGHT_3_PENALTY=3.885356
PRIMER_LEFT_3_SEQUENCE=CAGTTACTTGAATCGGAACAAC
PRIMER_RIGHT_3_SEQUENCE=GAATAATTTTCCGGCGATCTTG
PRIMER_LEFT_3=728,22
PRIMER_RIGHT_3=1650,22
PRIMER_LEFT_3_TM=56.457
PRIMER_RIGHT_3_TM=61.885
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=923
PRIMER_PAIR_4_PENALTY=10.839642
PRIMER_LEFT_4_PENALTY=5.542824
PRIMER_RIGHT_4_PENALTY=5.296819
PRIMER_LEFT_4_SEQUENCE=CAGTTACTTGAATCGGAACAAC
PRIMER_RIGHT_4_SEQUENCE=AATAATTTTCCGGCGATCTTGG
PRIMER_LEFT_4=728,22
PRIMER_RIGHT_4=1649,22
PRIMER_LEFT_4_TM=56.457
PRIMER_RIGHT_4_TM=63.297
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=922
PRIMER_PAIR_5_PENALTY=13.287446
PRIMER_LEFT_5_PENALTY=5.542824
PRIMER_RIGHT_5_PENALTY=7.744622
PRIMER_LEFT_5_SEQUENCE=CAGTTACTTGAATCGGAACAAC
PRIMER_RIGHT_5_SEQUENCE=GAATAATTTTCCGGCGATCTTGG
PRIMER_LEFT_5=728,22
PRIMER_RIGHT_5=1650,23
PRIMER_LEFT_5_TM=56.457
PRIMER_RIGHT_5_TM=64.745
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.446_4_5183343_5183372_po-0_can-0_409676_2302_TRUE
SEQUENCE_TARGET=950,129
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGATTTATCAANAATTNNTNGGANTTTTTATTTTTATACCATCATAATATATTTATTTAAATTAGTAATANAAGGCATTTTATTTACTATAAAANNTNTTTTATTAAAACTATTATATTTACTNATCCATTTTNTCTAAATATTNAAGGTNAAAAACTTACAACAATAATAAGTTACTNCTACANCAAANACAANTACAACAAAATNTCTAGAGCAAAANTTATATAGTTANAGCTCAACCAATNACTCNCATANAANNCANNNNNNCATTGGAAACTAACAAATGATACNNNNNNTTAGANGAACTTTAAATTTCATATTTTCAATAAAATGAGAGAGTTTGTAAAAGAATATACAATGATTTGTTTAACCAGCATTAGAGGTTTTTGAAGTTTTTCATNGCCAACTAAAAAGAAAAGGGTAAACATATGTTNAAGAACCTTAAAGAATNTTTGACNTGGAGTCATCCAGAGAGACATGAGGTCGAAGAAGCTTTTCGAATATATTTGGGAGAGNATCACGTCACGGCGGTTGCTCATGAAATAAACGTCCGGTGGTGCAAAAAATNTAGTGACGAATCACATATACTGTAGCTGNCTGAAGCAAAATGTATATGGTNNGAGCGACTAGTNNNATCATCTAATGTACAAGCTTCATCTTNGTCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATGGATCAAAGTTCAAACACNCTTAGAATTCATACCATCAATTTTCTAGACCTGGCAAGTGATNACTTCCTAGNCCTCACAAACNTAATTGTTGCGCNNNCNATAGTTCANAAGCTTGTGGCATNAANAGCTAAANNATCCAACTCATTCCAGATTCTTTTGCCCCTAGCTTCTTAGCAGNTTCTCTCACTTNACTTAACTGATCCCACATGCCTAACGTAGCATAAGCATTCGAAAGAGATGTATANACCGATGGATCATCCGGAAACNCCTTGAGTAATTTCTCAGCAANTACAGTAACCAGTCCGGCATTCTTGTGAGTTCCGCAAGCAGTGAGCAGAGACGTCCATATTGTTTTGTCGGGNTCCAACGGCATTTTNTTTATCAATTCNTNNGCNTCTTGGAACANCCCNGCTTGACCAAGAAGATCAACTACACAATAATAATGCTCNAGATACGGTTCAAGACCATATTCAGNTTTCATCGNTTCNAGGNTTGATCTTGCTTCTTCAAGNAGTCNAGAATGTCGGCAAGCNGAAAGAANCCCCAAAAAAGTTACCTNATTTGGTTCAANTCCANNANTNATCATCTTGTGGAAATACNGAAACGCTTCTTCGACTCGTCCGTTTTGTCCNAATCCAACAATTATCCCTGTCCAAGAGACAACATCTCTTTCNAACATACCATCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.217889
PRIMER_LEFT_0_PENALTY=1.108135
PRIMER_RIGHT_0_PENALTY=0.109754
PRIMER_LEFT_0_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_0_SEQUENCE=TGCTAAGAAGCTAGGGGCAA
PRIMER_LEFT_0=905,20
PRIMER_RIGHT_0=1518,20
PRIMER_LEFT_0_TM=58.892
PRIMER_RIGHT_0_TM=60.110
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=614
PRIMER_PAIR_1_PENALTY=1.267885
PRIMER_LEFT_1_PENALTY=1.108135
PRIMER_RIGHT_1_PENALTY=0.159750
PRIMER_LEFT_1_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_1_SEQUENCE=TTGCGGAACTCACAAGAATG
PRIMER_LEFT_1=905,20
PRIMER_RIGHT_1=1668,20
PRIMER_LEFT_1_TM=58.892
PRIMER_RIGHT_1_TM=59.840
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=764
PRIMER_PAIR_2_PENALTY=1.338537
PRIMER_LEFT_2_PENALTY=1.228784
PRIMER_RIGHT_2_PENALTY=0.109754
PRIMER_LEFT_2_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_2_SEQUENCE=TGCTAAGAAGCTAGGGGCAA
PRIMER_LEFT_2=896,20
PRIMER_RIGHT_2=1518,20
PRIMER_LEFT_2_TM=58.771
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=623
PRIMER_PAIR_3_PENALTY=1.361617
PRIMER_LEFT_3_PENALTY=1.108135
PRIMER_RIGHT_3_PENALTY=0.253482
PRIMER_LEFT_3_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_3_SEQUENCE=CTGCTAAGAAGCTAGGGGCA
PRIMER_LEFT_3=905,20
PRIMER_RIGHT_3=1519,20
PRIMER_LEFT_3_TM=58.892
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=615
PRIMER_PAIR_4_PENALTY=1.388533
PRIMER_LEFT_4_PENALTY=1.228784
PRIMER_RIGHT_4_PENALTY=0.159750
PRIMER_LEFT_4_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_4_SEQUENCE=TTGCGGAACTCACAAGAATG
PRIMER_LEFT_4=896,20
PRIMER_RIGHT_4=1668,20
PRIMER_LEFT_4_TM=58.771
PRIMER_RIGHT_4_TM=59.840
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=773
PRIMER_PAIR_5_PENALTY=1.482266
PRIMER_LEFT_5_PENALTY=1.228784
PRIMER_RIGHT_5_PENALTY=0.253482
PRIMER_LEFT_5_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_5_SEQUENCE=CTGCTAAGAAGCTAGGGGCA
PRIMER_LEFT_5=896,20
PRIMER_RIGHT_5=1519,20
PRIMER_LEFT_5_TM=58.771
PRIMER_RIGHT_5_TM=59.747
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.2000
PRIMER_RIGHT_5_END_STABILITY=11.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=624
PRIMER_PAIR_6_PENALTY=1.603482
PRIMER_LEFT_6_PENALTY=1.108135
PRIMER_RIGHT_6_PENALTY=0.495347
PRIMER_LEFT_6_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_6_SEQUENCE=GGGCAAAAGAATCTGGAATG
PRIMER_LEFT_6=905,20
PRIMER_RIGHT_6=1504,20
PRIMER_LEFT_6_TM=58.892
PRIMER_RIGHT_6_TM=59.505
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=600
PRIMER_PAIR_7_PENALTY=1.724130
PRIMER_LEFT_7_PENALTY=1.228784
PRIMER_RIGHT_7_PENALTY=0.495347
PRIMER_LEFT_7_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_7_SEQUENCE=GGGCAAAAGAATCTGGAATG
PRIMER_LEFT_7=896,20
PRIMER_RIGHT_7=1504,20
PRIMER_LEFT_7_TM=58.771
PRIMER_RIGHT_7_TM=59.505
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.2000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=609
PRIMER_PAIR_8_PENALTY=1.745467
PRIMER_LEFT_8_PENALTY=1.228784
PRIMER_RIGHT_8_PENALTY=0.516683
PRIMER_LEFT_8_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_8_SEQUENCE=GAATGCCGGACTGGTTACTG
PRIMER_LEFT_8=896,20
PRIMER_RIGHT_8=1653,20
PRIMER_LEFT_8_TM=58.771
PRIMER_RIGHT_8_TM=60.517
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.2000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=758
PRIMER_PAIR_9_PENALTY=1.745555
PRIMER_LEFT_9_PENALTY=1.108135
PRIMER_RIGHT_9_PENALTY=0.637420
PRIMER_LEFT_9_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_9_SEQUENCE=ACGTCTCTGCTCACTGCTTG
PRIMER_LEFT_9=905,20
PRIMER_RIGHT_9=1685,20
PRIMER_LEFT_9_TM=58.892
PRIMER_RIGHT_9_TM=59.363
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=781
PRIMER_PAIR_10_PENALTY=1.846906
PRIMER_LEFT_10_PENALTY=1.737152
PRIMER_RIGHT_10_PENALTY=0.109754
PRIMER_LEFT_10_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_10_SEQUENCE=TGCTAAGAAGCTAGGGGCAA
PRIMER_LEFT_10=904,21
PRIMER_RIGHT_10=1518,20
PRIMER_LEFT_10_TM=60.737
PRIMER_RIGHT_10_TM=60.110
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.9000
PRIMER_RIGHT_10_END_STABILITY=10.0000
PRIMER_PAIR_10_COMPL_ANY=2.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=615
PRIMER_PAIR_11_PENALTY=1.866203
PRIMER_LEFT_11_PENALTY=1.228784
PRIMER_RIGHT_11_PENALTY=0.637420
PRIMER_LEFT_11_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_11_SEQUENCE=ACGTCTCTGCTCACTGCTTG
PRIMER_LEFT_11=896,20
PRIMER_RIGHT_11=1685,20
PRIMER_LEFT_11_TM=58.771
PRIMER_RIGHT_11_TM=59.363
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.2000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=790
PRIMER_PAIR_12_PENALTY=1.876039
PRIMER_LEFT_12_PENALTY=1.108135
PRIMER_RIGHT_12_PENALTY=0.767904
PRIMER_LEFT_12_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_12_SEQUENCE=GGACGTCTCTGCTCACTGCT
PRIMER_LEFT_12=905,20
PRIMER_RIGHT_12=1687,20
PRIMER_LEFT_12_TM=58.892
PRIMER_RIGHT_12_TM=60.768
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=60.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=783
PRIMER_PAIR_13_PENALTY=1.896902
PRIMER_LEFT_13_PENALTY=1.737152
PRIMER_RIGHT_13_PENALTY=0.159750
PRIMER_LEFT_13_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_13_SEQUENCE=TTGCGGAACTCACAAGAATG
PRIMER_LEFT_13=904,21
PRIMER_RIGHT_13=1668,20
PRIMER_LEFT_13_TM=60.737
PRIMER_RIGHT_13_TM=59.840
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=765
PRIMER_PAIR_14_PENALTY=1.990634
PRIMER_LEFT_14_PENALTY=1.737152
PRIMER_RIGHT_14_PENALTY=0.253482
PRIMER_LEFT_14_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_14_SEQUENCE=CTGCTAAGAAGCTAGGGGCA
PRIMER_LEFT_14=904,21
PRIMER_RIGHT_14=1519,20
PRIMER_LEFT_14_TM=60.737
PRIMER_RIGHT_14_TM=59.747
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=11.2000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=616
PRIMER_PAIR_15_PENALTY=1.996687
PRIMER_LEFT_15_PENALTY=1.228784
PRIMER_RIGHT_15_PENALTY=0.767904
PRIMER_LEFT_15_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_15_SEQUENCE=GGACGTCTCTGCTCACTGCT
PRIMER_LEFT_15=896,20
PRIMER_RIGHT_15=1687,20
PRIMER_LEFT_15_TM=58.771
PRIMER_RIGHT_15_TM=60.768
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=60.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=792
PRIMER_PAIR_16_PENALTY=2.037552
PRIMER_LEFT_16_PENALTY=1.108135
PRIMER_RIGHT_16_PENALTY=0.929417
PRIMER_LEFT_16_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_16_SEQUENCE=AGGGGCAAAAGAATCTGGAA
PRIMER_LEFT_16=905,20
PRIMER_RIGHT_16=1506,20
PRIMER_LEFT_16_TM=58.892
PRIMER_RIGHT_16_TM=60.929
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=602
PRIMER_PAIR_17_PENALTY=2.111353
PRIMER_LEFT_17_PENALTY=1.108135
PRIMER_RIGHT_17_PENALTY=1.003218
PRIMER_LEFT_17_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_17_SEQUENCE=GCTAAGAAGCTAGGGGCAAAA
PRIMER_LEFT_17=905,20
PRIMER_RIGHT_17=1517,21
PRIMER_LEFT_17_TM=58.892
PRIMER_RIGHT_17_TM=59.997
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=2.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=613
PRIMER_PAIR_18_PENALTY=2.158201
PRIMER_LEFT_18_PENALTY=1.228784
PRIMER_RIGHT_18_PENALTY=0.929417
PRIMER_LEFT_18_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_18_SEQUENCE=AGGGGCAAAAGAATCTGGAA
PRIMER_LEFT_18=896,20
PRIMER_RIGHT_18=1506,20
PRIMER_LEFT_18_TM=58.771
PRIMER_RIGHT_18_TM=60.929
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.2000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=611
PRIMER_PAIR_19_PENALTY=2.159759
PRIMER_LEFT_19_PENALTY=1.108135
PRIMER_RIGHT_19_PENALTY=1.051624
PRIMER_LEFT_19_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_19_SEQUENCE=GCTACGTTAGGCATGTGGGA
PRIMER_LEFT_19=905,20
PRIMER_RIGHT_19=1562,20
PRIMER_LEFT_19_TM=58.892
PRIMER_RIGHT_19_TM=61.052
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=658
PRIMER_PAIR_20_PENALTY=2.232001
PRIMER_LEFT_20_PENALTY=1.228784
PRIMER_RIGHT_20_PENALTY=1.003218
PRIMER_LEFT_20_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_20_SEQUENCE=GCTAAGAAGCTAGGGGCAAAA
PRIMER_LEFT_20=896,20
PRIMER_RIGHT_20=1517,21
PRIMER_LEFT_20_TM=58.771
PRIMER_RIGHT_20_TM=59.997
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.2000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=622
PRIMER_PAIR_21_PENALTY=2.232499
PRIMER_LEFT_21_PENALTY=1.737152
PRIMER_RIGHT_21_PENALTY=0.495347
PRIMER_LEFT_21_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_21_SEQUENCE=GGGCAAAAGAATCTGGAATG
PRIMER_LEFT_21=904,21
PRIMER_RIGHT_21=1504,20
PRIMER_LEFT_21_TM=60.737
PRIMER_RIGHT_21_TM=59.505
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=601
PRIMER_PAIR_22_PENALTY=2.240403
PRIMER_LEFT_22_PENALTY=1.228784
PRIMER_RIGHT_22_PENALTY=1.011619
PRIMER_LEFT_22_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_22_SEQUENCE=AAGAATGCCGGACTGGTTACT
PRIMER_LEFT_22=896,20
PRIMER_RIGHT_22=1655,21
PRIMER_LEFT_22_TM=58.771
PRIMER_RIGHT_22_TM=60.012
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.2000
PRIMER_RIGHT_22_END_STABILITY=5.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=760
PRIMER_PAIR_23_PENALTY=2.280407
PRIMER_LEFT_23_PENALTY=1.228784
PRIMER_RIGHT_23_PENALTY=1.051624
PRIMER_LEFT_23_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_23_SEQUENCE=GCTACGTTAGGCATGTGGGA
PRIMER_LEFT_23=896,20
PRIMER_RIGHT_23=1562,20
PRIMER_LEFT_23_TM=58.771
PRIMER_RIGHT_23_TM=61.052
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.2000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=667
PRIMER_PAIR_24_PENALTY=2.323175
PRIMER_LEFT_24_PENALTY=1.108135
PRIMER_RIGHT_24_PENALTY=1.215039
PRIMER_LEFT_24_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_24_SEQUENCE=CTAGGGGCAAAAGAATCTGG
PRIMER_LEFT_24=905,20
PRIMER_RIGHT_24=1508,20
PRIMER_LEFT_24_TM=58.892
PRIMER_RIGHT_24_TM=58.785
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.9000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=604
PRIMER_PAIR_25_PENALTY=2.366328
PRIMER_LEFT_25_PENALTY=1.108135
PRIMER_RIGHT_25_PENALTY=1.258193
PRIMER_LEFT_25_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_25_SEQUENCE=GCTAAGAAGCTAGGGGCAAA
PRIMER_LEFT_25=905,20
PRIMER_RIGHT_25=1517,20
PRIMER_LEFT_25_TM=58.892
PRIMER_RIGHT_25_TM=58.742
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.9000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=613
PRIMER_PAIR_26_PENALTY=2.374572
PRIMER_LEFT_26_PENALTY=1.737152
PRIMER_RIGHT_26_PENALTY=0.637420
PRIMER_LEFT_26_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_26_SEQUENCE=ACGTCTCTGCTCACTGCTTG
PRIMER_LEFT_26=904,21
PRIMER_RIGHT_26=1685,20
PRIMER_LEFT_26_TM=60.737
PRIMER_RIGHT_26_TM=59.363
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=782
PRIMER_PAIR_27_PENALTY=2.430876
PRIMER_LEFT_27_PENALTY=1.108135
PRIMER_RIGHT_27_PENALTY=1.322740
PRIMER_LEFT_27_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_27_SEQUENCE=ATGCTACGTTAGGCATGTGG
PRIMER_LEFT_27=905,20
PRIMER_RIGHT_27=1564,20
PRIMER_LEFT_27_TM=58.892
PRIMER_RIGHT_27_TM=58.677
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=660
PRIMER_PAIR_28_PENALTY=2.443823
PRIMER_LEFT_28_PENALTY=1.228784
PRIMER_RIGHT_28_PENALTY=1.215039
PRIMER_LEFT_28_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_28_SEQUENCE=CTAGGGGCAAAAGAATCTGG
PRIMER_LEFT_28=896,20
PRIMER_RIGHT_28=1508,20
PRIMER_LEFT_28_TM=58.771
PRIMER_RIGHT_28_TM=58.785
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.2000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=613
PRIMER_PAIR_29_PENALTY=2.486977
PRIMER_LEFT_29_PENALTY=1.228784
PRIMER_RIGHT_29_PENALTY=1.258193
PRIMER_LEFT_29_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_29_SEQUENCE=GCTAAGAAGCTAGGGGCAAA
PRIMER_LEFT_29=896,20
PRIMER_RIGHT_29=1517,20
PRIMER_LEFT_29_TM=58.771
PRIMER_RIGHT_29_TM=58.742
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.2000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=622
PRIMER_PAIR_30_PENALTY=2.511796
PRIMER_LEFT_30_PENALTY=2.402042
PRIMER_RIGHT_30_PENALTY=0.109754
PRIMER_LEFT_30_SEQUENCE=CAGAGAGACATGAGGTCGAAGA
PRIMER_RIGHT_30_SEQUENCE=TGCTAAGAAGCTAGGGGCAA
PRIMER_LEFT_30=837,22
PRIMER_RIGHT_30=1518,20
PRIMER_LEFT_30_TM=59.598
PRIMER_RIGHT_30_TM=60.110
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=10.0000
PRIMER_PAIR_30_COMPL_ANY=2.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=682
PRIMER_PAIR_31_PENALTY=2.551524
PRIMER_LEFT_31_PENALTY=1.228784
PRIMER_RIGHT_31_PENALTY=1.322740
PRIMER_LEFT_31_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_31_SEQUENCE=ATGCTACGTTAGGCATGTGG
PRIMER_LEFT_31=896,20
PRIMER_RIGHT_31=1564,20
PRIMER_LEFT_31_TM=58.771
PRIMER_RIGHT_31_TM=58.677
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.2000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=669
PRIMER_PAIR_32_PENALTY=2.557161
PRIMER_LEFT_32_PENALTY=1.228784
PRIMER_RIGHT_32_PENALTY=1.328377
PRIMER_LEFT_32_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_32_SEQUENCE=AGAATGCCGGACTGGTTACT
PRIMER_LEFT_32=896,20
PRIMER_RIGHT_32=1654,20
PRIMER_LEFT_32_TM=58.771
PRIMER_RIGHT_32_TM=58.672
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.2000
PRIMER_RIGHT_32_END_STABILITY=5.7000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=759
PRIMER_PAIR_33_PENALTY=2.561792
PRIMER_LEFT_33_PENALTY=2.402042
PRIMER_RIGHT_33_PENALTY=0.159750
PRIMER_LEFT_33_SEQUENCE=CAGAGAGACATGAGGTCGAAGA
PRIMER_RIGHT_33_SEQUENCE=TTGCGGAACTCACAAGAATG
PRIMER_LEFT_33=837,22
PRIMER_RIGHT_33=1668,20
PRIMER_LEFT_33_TM=59.598
PRIMER_RIGHT_33_TM=59.840
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=832
PRIMER_PAIR_34_PENALTY=2.563439
PRIMER_LEFT_34_PENALTY=1.108135
PRIMER_RIGHT_34_PENALTY=1.455303
PRIMER_LEFT_34_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_34_SEQUENCE=CGTTAGGCATGTGGGATCAG
PRIMER_LEFT_34=905,20
PRIMER_RIGHT_34=1558,20
PRIMER_LEFT_34_TM=58.892
PRIMER_RIGHT_34_TM=61.455
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.9000
PRIMER_RIGHT_34_END_STABILITY=6.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=654
PRIMER_PAIR_35_PENALTY=2.598121
PRIMER_LEFT_35_PENALTY=1.108135
PRIMER_RIGHT_35_PENALTY=1.489986
PRIMER_LEFT_35_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_35_SEQUENCE=AAGAAGCTAGGGGCAAAAGAA
PRIMER_LEFT_35=905,20
PRIMER_RIGHT_35=1514,21
PRIMER_LEFT_35_TM=58.892
PRIMER_RIGHT_35_TM=59.510
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.9000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=610
PRIMER_PAIR_36_PENALTY=2.601744
PRIMER_LEFT_36_PENALTY=1.108135
PRIMER_RIGHT_36_PENALTY=1.493609
PRIMER_LEFT_36_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_36_SEQUENCE=GCTTGCGGAACTCACAAGAA
PRIMER_LEFT_36=905,20
PRIMER_RIGHT_36=1670,20
PRIMER_LEFT_36_TM=58.892
PRIMER_RIGHT_36_TM=61.494
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=766
PRIMER_PAIR_37_PENALTY=2.655524
PRIMER_LEFT_37_PENALTY=2.402042
PRIMER_RIGHT_37_PENALTY=0.253482
PRIMER_LEFT_37_SEQUENCE=CAGAGAGACATGAGGTCGAAGA
PRIMER_RIGHT_37_SEQUENCE=CTGCTAAGAAGCTAGGGGCA
PRIMER_LEFT_37=837,22
PRIMER_RIGHT_37=1519,20
PRIMER_LEFT_37_TM=59.598
PRIMER_RIGHT_37_TM=59.747
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=11.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=683
PRIMER_PAIR_38_PENALTY=2.666569
PRIMER_LEFT_38_PENALTY=1.737152
PRIMER_RIGHT_38_PENALTY=0.929417
PRIMER_LEFT_38_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_38_SEQUENCE=AGGGGCAAAAGAATCTGGAA
PRIMER_LEFT_38=904,21
PRIMER_RIGHT_38=1506,20
PRIMER_LEFT_38_TM=60.737
PRIMER_RIGHT_38_TM=60.929
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.9000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=603
PRIMER_PAIR_39_PENALTY=2.684087
PRIMER_LEFT_39_PENALTY=1.228784
PRIMER_RIGHT_39_PENALTY=1.455303
PRIMER_LEFT_39_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_39_SEQUENCE=CGTTAGGCATGTGGGATCAG
PRIMER_LEFT_39=896,20
PRIMER_RIGHT_39=1558,20
PRIMER_LEFT_39_TM=58.771
PRIMER_RIGHT_39_TM=61.455
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.2000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=663
PRIMER_PAIR_40_PENALTY=2.718769
PRIMER_LEFT_40_PENALTY=1.228784
PRIMER_RIGHT_40_PENALTY=1.489986
PRIMER_LEFT_40_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_40_SEQUENCE=AAGAAGCTAGGGGCAAAAGAA
PRIMER_LEFT_40=896,20
PRIMER_RIGHT_40=1514,21
PRIMER_LEFT_40_TM=58.771
PRIMER_RIGHT_40_TM=59.510
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.2000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=619
PRIMER_PAIR_41_PENALTY=2.722392
PRIMER_LEFT_41_PENALTY=1.228784
PRIMER_RIGHT_41_PENALTY=1.493609
PRIMER_LEFT_41_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_41_SEQUENCE=GCTTGCGGAACTCACAAGAA
PRIMER_LEFT_41=896,20
PRIMER_RIGHT_41=1670,20
PRIMER_LEFT_41_TM=58.771
PRIMER_RIGHT_41_TM=61.494
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.2000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=775
PRIMER_PAIR_42_PENALTY=2.740370
PRIMER_LEFT_42_PENALTY=1.737152
PRIMER_RIGHT_42_PENALTY=1.003218
PRIMER_LEFT_42_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_42_SEQUENCE=GCTAAGAAGCTAGGGGCAAAA
PRIMER_LEFT_42=904,21
PRIMER_RIGHT_42=1517,21
PRIMER_LEFT_42_TM=60.737
PRIMER_RIGHT_42_TM=59.997
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=2.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=614
PRIMER_PAIR_43_PENALTY=2.774768
PRIMER_LEFT_43_PENALTY=1.108135
PRIMER_RIGHT_43_PENALTY=1.666632
PRIMER_LEFT_43_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_43_SEQUENCE=GACGTCTCTGCTCACTGCTT
PRIMER_LEFT_43=905,20
PRIMER_RIGHT_43=1686,20
PRIMER_LEFT_43_TM=58.892
PRIMER_RIGHT_43_TM=58.333
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=782
PRIMER_PAIR_44_PENALTY=2.788776
PRIMER_LEFT_44_PENALTY=1.737152
PRIMER_RIGHT_44_PENALTY=1.051624
PRIMER_LEFT_44_SEQUENCE=TCATGAAATAAACGTCCGGTG
PRIMER_RIGHT_44_SEQUENCE=GCTACGTTAGGCATGTGGGA
PRIMER_LEFT_44=904,21
PRIMER_RIGHT_44=1562,20
PRIMER_LEFT_44_TM=60.737
PRIMER_RIGHT_44_TM=61.052
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.9000
PRIMER_RIGHT_44_END_STABILITY=9.7000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=659
PRIMER_PAIR_45_PENALTY=2.895047
PRIMER_LEFT_45_PENALTY=1.108135
PRIMER_RIGHT_45_PENALTY=1.786912
PRIMER_LEFT_45_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_45_SEQUENCE=AGAAGCTAGGGGCAAAAGAA
PRIMER_LEFT_45=905,20
PRIMER_RIGHT_45=1513,20
PRIMER_LEFT_45_TM=58.892
PRIMER_RIGHT_45_TM=58.213
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.9000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=609
PRIMER_PAIR_46_PENALTY=2.895047
PRIMER_LEFT_46_PENALTY=1.108135
PRIMER_RIGHT_46_PENALTY=1.786912
PRIMER_LEFT_46_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_46_SEQUENCE=AAGAAGCTAGGGGCAAAAGA
PRIMER_LEFT_46=905,20
PRIMER_RIGHT_46=1514,20
PRIMER_LEFT_46_TM=58.892
PRIMER_RIGHT_46_TM=58.213
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=610
PRIMER_PAIR_47_PENALTY=2.895416
PRIMER_LEFT_47_PENALTY=1.228784
PRIMER_RIGHT_47_PENALTY=1.666632
PRIMER_LEFT_47_SEQUENCE=GCGGTTGCTCATGAAATAAA
PRIMER_RIGHT_47_SEQUENCE=GACGTCTCTGCTCACTGCTT
PRIMER_LEFT_47=896,20
PRIMER_RIGHT_47=1686,20
PRIMER_LEFT_47_TM=58.771
PRIMER_RIGHT_47_TM=58.333
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.2000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=791
PRIMER_PAIR_48_PENALTY=2.897389
PRIMER_LEFT_48_PENALTY=2.402042
PRIMER_RIGHT_48_PENALTY=0.495347
PRIMER_LEFT_48_SEQUENCE=CAGAGAGACATGAGGTCGAAGA
PRIMER_RIGHT_48_SEQUENCE=GGGCAAAAGAATCTGGAATG
PRIMER_LEFT_48=837,22
PRIMER_RIGHT_48=1504,20
PRIMER_LEFT_48_TM=59.598
PRIMER_RIGHT_48_TM=59.505
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=668
PRIMER_PAIR_49_PENALTY=2.903722
PRIMER_LEFT_49_PENALTY=1.108135
PRIMER_RIGHT_49_PENALTY=1.795587
PRIMER_LEFT_49_SEQUENCE=CATGAAATAAACGTCCGGTG
PRIMER_RIGHT_49_SEQUENCE=CTCACAAGAATGCCGGACTG
PRIMER_LEFT_49=905,20
PRIMER_RIGHT_49=1660,20
PRIMER_LEFT_49_TM=58.892
PRIMER_RIGHT_49_TM=61.796
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=756
=
SEQUENCE_ID=MAGIC.446_2_5499866_5499887_ler-0_tsu-0_31903_160934_TRUE
SEQUENCE_TARGET=950,121
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANCGATCCTGATTTGAATAAGTATTAGAACAAGTCGNCTTTGATCTGAATTGGTATCAGATGGAACCTAAATCAAGATAAGGTGATCTTGATTTGGGAAANAACATGGTTANTTCCACTNTTCATTNANTTCTTTNACAATTTCTTCTATTTGAGTCAACAGTTTTATTGGAATTACTTTTGTATATACTGTGTAAAACTCCTTATGATAAAATATAATTGAATTATTTNTGTAGTGTGTAATTAGTTAATTCCNTGAGTATTTAGAANCTTTCGTTAAATAACTTTTAGTACTCANTTTTTGAAAATTTTAAATAGAATTATAATTTAGATTCAAATACAATNNNTATTTTTAGTNGGTTAGTTTGGTGTAGTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_24049117_24049517_sf-2_oy-0_11721_617728_TRUE
SEQUENCE_TARGET=950,500
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANCTGATAAGAACCAACAGATCAACTCTTCCACCTCTNCTNANCGCTNNTGAGGTNTACANAGTTATTCAGTTTCCGCAGTTNGAAACNAATGAAACCGACGGTANNNNTGNTNCNAGGTTATNNTANNACGATCTATGCTTTCTTATGATAGTATGGAAAGAAATNNNTNATAAACTCTNAAAGAGTATTCAAAACGTTTTATNTATTCTTTCTAACATCACNAAAACANTTNTATNNNNNNNNNNNCTGTGNNAANNNNNNNAGCNNCCTATGNATTGAGTANAATNAACTGGCAANGNGATCCATGNNTCCCTCNACANNTTANGTGGGANGGNTTAAACTGCAGCANCNNANNTNNNNNNNNNNCACCAAGAATCACTNCTTTGTAANNNANNAGTTCACNAGNNNNNNCNNNNNNNNNNNNNNNTNATCATGATCNNATTNTTGAATNTGGTTTTGNAAATNTCACCAGAAACTTGTCNTCNAGTGGTTTAACTGNAACNNNNNCAGCTGCCATTCAAAATCTTACGANACTAGAAAAGCTGTAAGTATGTNTTGTNGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANATTCTCTNTTNNTNNCTNTAGGGACTTGTCNAATAANAANTTGACNGGAGAGGTGCCAGAATTTCTANGCAACATNANATCGNTGNTGNTCATGTAANTNTNTNNNNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCTTAANGGTNCAATTCCTCAATCNCTACANAGGAANGGACTNGAGCTNNTGTAAGAANNTTCCCACATNNNNNNNNNNNNNNNNNNNNCATTATCCACAACCNTNNTGTNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANCANNTCTGGCAAGAGCTTTCCAGTNACNATNGTTGCATCAGTTGGTTCTGCAGCCATNCTNATTGTTGTGNTGGTTCTNNTNCTTTTTCTCCGAAAGAAAAANNNNNCGNCTGNTGAAGGTATTTGCAAGAGAGNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGATCTAACAGTTNTTCNCCCGCGTCCAAGTAGGCCNACGATGAATGTTCCTTATGNTAANTCACCTGAGCCNTCAATCGAGATGAAAAAGAGAANGTTTACTTATTNAGAGGTTANNAAAATGACNAATNANTTCGNAAGAGTTGTAGGGGAAGGAGGATTTGGCGTNGTCTGNCANGGNACTGTAAATGGNTCTGAACAGGTNGCTGTTAAANTACTNTCNCAATCATCAACTCAAGGNTATAAAGAGTTCAAGGCAGAGGTATTTCCANTACAATCTTTCCAACTAGTATCTGTTTTNAACANCANAAANCTAANNNNNTTNNNNNNNNNNCNTTGCATGANTGCNTTAGGTTGATCTTCTTCTGAGAGTTCACCATACNAATTTGGTAAGCCTTGTCGGATANTGTGATGANGGAGANCACTTGGCCCTNATCTANGAGTTTGTNCCCAATGGANACTTAAGACAACATTTGTCTGGTAANACTNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATANAGGAAAAGGAGGTNAACCCATCGTCAATTGGGGTACTCGGCTAAGAATAGCNGNNGAGGCAGCACTAGGTTTGTACCAAGTTACANNGTAGAACTNNNATNCANTCNATTGGTTTTACAANNNNNNNNNNNNNNNNNNGAATCATTNTGCATAGGTTTGGAGTACTTACACATTGGATGCACACCACCAATGNTTCATAGNGATGTCAAAACTACAAACATATTGNTGGNCGAGCATTATAAGGCCAANCTNGCTGATTTTGGNCTCTCNAGNTCTTTCCCNGTTGGAGGNGAANCTCATGTTTCNACNGTGATTGNTGGTACNCCTGGNTACCTTGATCCAGAGTAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGAGAAAAGNGATGTNTACAGTTTCGGGATTGTTTTATTNGANATGATCNCAAACCAAGCAGTNATTGATCGAAACCGCANAAAGTCTCACATAACACAA
PRIMER_LEFT_NUM_RETURNED=38
PRIMER_RIGHT_NUM_RETURNED=38
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=38
PRIMER_PAIR_0_PENALTY=4.801153
PRIMER_LEFT_0_PENALTY=3.098855
PRIMER_RIGHT_0_PENALTY=1.702298
PRIMER_LEFT_0_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_0_SEQUENCE=TGGAAATACCTCTGCCTTGA
PRIMER_LEFT_0=577,22
PRIMER_RIGHT_0=1554,20
PRIMER_LEFT_0_TM=58.901
PRIMER_RIGHT_0_TM=58.298
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=978
PRIMER_PAIR_1_PENALTY=5.581639
PRIMER_LEFT_1_PENALTY=3.098855
PRIMER_RIGHT_1_PENALTY=2.482784
PRIMER_LEFT_1_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_1_SEQUENCE=TGGAAATACCTCTGCCTTGAAC
PRIMER_LEFT_1=577,22
PRIMER_RIGHT_1=1554,22
PRIMER_LEFT_1_TM=58.901
PRIMER_RIGHT_1_TM=60.483
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=978
PRIMER_PAIR_2_PENALTY=6.300476
PRIMER_LEFT_2_PENALTY=3.098855
PRIMER_RIGHT_2_PENALTY=3.201621
PRIMER_LEFT_2_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_2_SEQUENCE=GGAAATACCTCTGCCTTGAAC
PRIMER_LEFT_2=577,22
PRIMER_RIGHT_2=1553,21
PRIMER_LEFT_2_TM=58.901
PRIMER_RIGHT_2_TM=57.798
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=977
PRIMER_PAIR_3_PENALTY=6.315875
PRIMER_LEFT_3_PENALTY=3.098855
PRIMER_RIGHT_3_PENALTY=3.217019
PRIMER_LEFT_3_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_3_SEQUENCE=GGAAATACCTCTGCCTTGAACT
PRIMER_LEFT_3=577,22
PRIMER_RIGHT_3=1553,22
PRIMER_LEFT_3_TM=58.901
PRIMER_RIGHT_3_TM=58.783
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=977
PRIMER_PAIR_4_PENALTY=6.656789
PRIMER_LEFT_4_PENALTY=4.954492
PRIMER_RIGHT_4_PENALTY=1.702298
PRIMER_LEFT_4_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_4_SEQUENCE=TGGAAATACCTCTGCCTTGA
PRIMER_LEFT_4=579,20
PRIMER_RIGHT_4=1554,20
PRIMER_LEFT_4_TM=55.046
PRIMER_RIGHT_4_TM=58.298
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=976
PRIMER_PAIR_5_PENALTY=7.414582
PRIMER_LEFT_5_PENALTY=3.098855
PRIMER_RIGHT_5_PENALTY=4.315727
PRIMER_LEFT_5_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_5_SEQUENCE=ACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_5=577,22
PRIMER_RIGHT_5=1547,20
PRIMER_LEFT_5_TM=58.901
PRIMER_RIGHT_5_TM=55.684
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=971
PRIMER_PAIR_6_PENALTY=7.425582
PRIMER_LEFT_6_PENALTY=3.098855
PRIMER_RIGHT_6_PENALTY=4.326727
PRIMER_LEFT_6_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_6_SEQUENCE=TGGAAATACCTCTGCCTTGAACT
PRIMER_LEFT_6=577,22
PRIMER_RIGHT_6=1554,23
PRIMER_LEFT_6_TM=58.901
PRIMER_RIGHT_6_TM=61.327
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=978
PRIMER_PAIR_7_PENALTY=7.437275
PRIMER_LEFT_7_PENALTY=4.954492
PRIMER_RIGHT_7_PENALTY=2.482784
PRIMER_LEFT_7_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_7_SEQUENCE=TGGAAATACCTCTGCCTTGAAC
PRIMER_LEFT_7=579,20
PRIMER_RIGHT_7=1554,22
PRIMER_LEFT_7_TM=55.046
PRIMER_RIGHT_7_TM=60.483
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=976
PRIMER_PAIR_8_PENALTY=7.736699
PRIMER_LEFT_8_PENALTY=3.098855
PRIMER_RIGHT_8_PENALTY=4.637844
PRIMER_LEFT_8_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_8_SEQUENCE=GAAATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_8=577,22
PRIMER_RIGHT_8=1552,24
PRIMER_LEFT_8_TM=58.901
PRIMER_RIGHT_8_TM=59.362
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=41.667
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=5.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=976
PRIMER_PAIR_9_PENALTY=7.984220
PRIMER_LEFT_9_PENALTY=3.098855
PRIMER_RIGHT_9_PENALTY=4.885365
PRIMER_LEFT_9_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_9_SEQUENCE=GAAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_9=577,22
PRIMER_RIGHT_9=1552,23
PRIMER_LEFT_9_TM=58.901
PRIMER_RIGHT_9_TM=58.115
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=976
PRIMER_PAIR_10_PENALTY=8.156112
PRIMER_LEFT_10_PENALTY=4.954492
PRIMER_RIGHT_10_PENALTY=3.201621
PRIMER_LEFT_10_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_10_SEQUENCE=GGAAATACCTCTGCCTTGAAC
PRIMER_LEFT_10=579,20
PRIMER_RIGHT_10=1553,21
PRIMER_LEFT_10_TM=55.046
PRIMER_RIGHT_10_TM=57.798
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=5.7000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=975
PRIMER_PAIR_11_PENALTY=8.171511
PRIMER_LEFT_11_PENALTY=4.954492
PRIMER_RIGHT_11_PENALTY=3.217019
PRIMER_LEFT_11_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_11_SEQUENCE=GGAAATACCTCTGCCTTGAACT
PRIMER_LEFT_11=579,20
PRIMER_RIGHT_11=1553,22
PRIMER_LEFT_11_TM=55.046
PRIMER_RIGHT_11_TM=58.783
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=975
PRIMER_PAIR_12_PENALTY=8.239819
PRIMER_LEFT_12_PENALTY=3.098855
PRIMER_RIGHT_12_PENALTY=5.140964
PRIMER_LEFT_12_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_12_SEQUENCE=TACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_12=577,22
PRIMER_RIGHT_12=1548,21
PRIMER_LEFT_12_TM=58.901
PRIMER_RIGHT_12_TM=55.859
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=972
PRIMER_PAIR_13_PENALTY=8.386302
PRIMER_LEFT_13_PENALTY=3.098855
PRIMER_RIGHT_13_PENALTY=5.287447
PRIMER_LEFT_13_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_13_SEQUENCE=GGAAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_13=577,22
PRIMER_RIGHT_13=1553,24
PRIMER_LEFT_13_TM=58.901
PRIMER_RIGHT_13_TM=61.287
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=45.833
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=6.1000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=977
PRIMER_PAIR_14_PENALTY=8.595561
PRIMER_LEFT_14_PENALTY=3.098855
PRIMER_RIGHT_14_PENALTY=5.496706
PRIMER_LEFT_14_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_14_SEQUENCE=GAAATACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_14=577,22
PRIMER_RIGHT_14=1552,25
PRIMER_LEFT_14_TM=58.901
PRIMER_RIGHT_14_TM=60.497
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=5.7000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=976
PRIMER_PAIR_15_PENALTY=8.769198
PRIMER_LEFT_15_PENALTY=3.098855
PRIMER_RIGHT_15_PENALTY=5.670343
PRIMER_LEFT_15_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_15_SEQUENCE=ATACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_15=577,22
PRIMER_RIGHT_15=1549,22
PRIMER_LEFT_15_TM=58.901
PRIMER_RIGHT_15_TM=56.330
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=5.7000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=973
PRIMER_PAIR_16_PENALTY=8.769198
PRIMER_LEFT_16_PENALTY=3.098855
PRIMER_RIGHT_16_PENALTY=5.670343
PRIMER_LEFT_16_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_16_SEQUENCE=AATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_16=577,22
PRIMER_RIGHT_16=1550,22
PRIMER_LEFT_16_TM=58.901
PRIMER_RIGHT_16_TM=56.330
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=974
PRIMER_PAIR_17_PENALTY=8.769198
PRIMER_LEFT_17_PENALTY=3.098855
PRIMER_RIGHT_17_PENALTY=5.670343
PRIMER_LEFT_17_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_17_SEQUENCE=AAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_17=577,22
PRIMER_RIGHT_17=1551,22
PRIMER_LEFT_17_TM=58.901
PRIMER_RIGHT_17_TM=56.330
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=40.909
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=6.1000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=975
PRIMER_PAIR_18_PENALTY=8.880519
PRIMER_LEFT_18_PENALTY=3.098855
PRIMER_RIGHT_18_PENALTY=5.781664
PRIMER_LEFT_18_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_18_SEQUENCE=GAAATACCTCTGCCTTGAACT
PRIMER_LEFT_18=577,22
PRIMER_RIGHT_18=1552,21
PRIMER_LEFT_18_TM=58.901
PRIMER_RIGHT_18_TM=55.218
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=5.7000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=976
PRIMER_PAIR_19_PENALTY=9.270218
PRIMER_LEFT_19_PENALTY=4.954492
PRIMER_RIGHT_19_PENALTY=4.315727
PRIMER_LEFT_19_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_19_SEQUENCE=ACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_19=579,20
PRIMER_RIGHT_19=1547,20
PRIMER_LEFT_19_TM=55.046
PRIMER_RIGHT_19_TM=55.684
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.7000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=969
PRIMER_PAIR_20_PENALTY=9.281218
PRIMER_LEFT_20_PENALTY=4.954492
PRIMER_RIGHT_20_PENALTY=4.326727
PRIMER_LEFT_20_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_20_SEQUENCE=TGGAAATACCTCTGCCTTGAACT
PRIMER_LEFT_20=579,20
PRIMER_RIGHT_20=1554,23
PRIMER_LEFT_20_TM=55.046
PRIMER_RIGHT_20_TM=61.327
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=43.478
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=5.7000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=976
PRIMER_PAIR_21_PENALTY=9.592336
PRIMER_LEFT_21_PENALTY=4.954492
PRIMER_RIGHT_21_PENALTY=4.637844
PRIMER_LEFT_21_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_21_SEQUENCE=GAAATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_21=579,20
PRIMER_RIGHT_21=1552,24
PRIMER_LEFT_21_TM=55.046
PRIMER_RIGHT_21_TM=59.362
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=41.667
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=5.7000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=974
PRIMER_PAIR_22_PENALTY=9.839856
PRIMER_LEFT_22_PENALTY=4.954492
PRIMER_RIGHT_22_PENALTY=4.885365
PRIMER_LEFT_22_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_22_SEQUENCE=GAAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_22=579,20
PRIMER_RIGHT_22=1552,23
PRIMER_LEFT_22_TM=55.046
PRIMER_RIGHT_22_TM=58.115
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=43.478
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.7000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=974
PRIMER_PAIR_23_PENALTY=10.095455
PRIMER_LEFT_23_PENALTY=4.954492
PRIMER_RIGHT_23_PENALTY=5.140964
PRIMER_LEFT_23_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_23_SEQUENCE=TACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_23=579,20
PRIMER_RIGHT_23=1548,21
PRIMER_LEFT_23_TM=55.046
PRIMER_RIGHT_23_TM=55.859
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=5.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=970
PRIMER_PAIR_24_PENALTY=10.241939
PRIMER_LEFT_24_PENALTY=4.954492
PRIMER_RIGHT_24_PENALTY=5.287447
PRIMER_LEFT_24_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_24_SEQUENCE=GGAAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_24=579,20
PRIMER_RIGHT_24=1553,24
PRIMER_LEFT_24_TM=55.046
PRIMER_RIGHT_24_TM=61.287
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.833
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=5.7000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=975
PRIMER_PAIR_25_PENALTY=10.428567
PRIMER_LEFT_25_PENALTY=3.098855
PRIMER_RIGHT_25_PENALTY=7.329712
PRIMER_LEFT_25_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_25_SEQUENCE=GGAAATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_25=577,22
PRIMER_RIGHT_25=1553,25
PRIMER_LEFT_25_TM=58.901
PRIMER_RIGHT_25_TM=62.330
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=44.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=5.7000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=977
PRIMER_PAIR_26_PENALTY=10.451197
PRIMER_LEFT_26_PENALTY=4.954492
PRIMER_RIGHT_26_PENALTY=5.496706
PRIMER_LEFT_26_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_26_SEQUENCE=GAAATACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_26=579,20
PRIMER_RIGHT_26=1552,25
PRIMER_LEFT_26_TM=55.046
PRIMER_RIGHT_26_TM=60.497
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=5.7000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=974
PRIMER_PAIR_27_PENALTY=10.624834
PRIMER_LEFT_27_PENALTY=4.954492
PRIMER_RIGHT_27_PENALTY=5.670343
PRIMER_LEFT_27_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_27_SEQUENCE=ATACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_27=579,20
PRIMER_RIGHT_27=1549,22
PRIMER_LEFT_27_TM=55.046
PRIMER_RIGHT_27_TM=56.330
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=971
PRIMER_PAIR_28_PENALTY=10.624834
PRIMER_LEFT_28_PENALTY=4.954492
PRIMER_RIGHT_28_PENALTY=5.670343
PRIMER_LEFT_28_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_28_SEQUENCE=AATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_28=579,20
PRIMER_RIGHT_28=1550,22
PRIMER_LEFT_28_TM=55.046
PRIMER_RIGHT_28_TM=56.330
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=5.7000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=972
PRIMER_PAIR_29_PENALTY=10.624834
PRIMER_LEFT_29_PENALTY=4.954492
PRIMER_RIGHT_29_PENALTY=5.670343
PRIMER_LEFT_29_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_29_SEQUENCE=AAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_29=579,20
PRIMER_RIGHT_29=1551,22
PRIMER_LEFT_29_TM=55.046
PRIMER_RIGHT_29_TM=56.330
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=5.7000
PRIMER_RIGHT_29_END_STABILITY=6.1000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=973
PRIMER_PAIR_30_PENALTY=10.736156
PRIMER_LEFT_30_PENALTY=4.954492
PRIMER_RIGHT_30_PENALTY=5.781664
PRIMER_LEFT_30_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_30_SEQUENCE=GAAATACCTCTGCCTTGAACT
PRIMER_LEFT_30=579,20
PRIMER_RIGHT_30=1552,21
PRIMER_LEFT_30_TM=55.046
PRIMER_RIGHT_30_TM=55.218
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=5.7000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=974
PRIMER_PAIR_31_PENALTY=11.700816
PRIMER_LEFT_31_PENALTY=3.098855
PRIMER_RIGHT_31_PENALTY=8.601961
PRIMER_LEFT_31_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_31_SEQUENCE=TGGAAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_31=577,22
PRIMER_RIGHT_31=1554,25
PRIMER_LEFT_31_TM=58.901
PRIMER_RIGHT_31_TM=63.602
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=44.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.7000
PRIMER_RIGHT_31_END_STABILITY=6.1000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=978
PRIMER_PAIR_32_PENALTY=12.284203
PRIMER_LEFT_32_PENALTY=4.954492
PRIMER_RIGHT_32_PENALTY=7.329712
PRIMER_LEFT_32_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_32_SEQUENCE=GGAAATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_32=579,20
PRIMER_RIGHT_32=1553,25
PRIMER_LEFT_32_TM=55.046
PRIMER_RIGHT_32_TM=62.330
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=44.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=5.7000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=975
PRIMER_PAIR_33_PENALTY=12.380958
PRIMER_LEFT_33_PENALTY=3.098855
PRIMER_RIGHT_33_PENALTY=9.282103
PRIMER_LEFT_33_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_33_SEQUENCE=GGAAATACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_33=577,22
PRIMER_RIGHT_33=1553,26
PRIMER_LEFT_33_TM=58.901
PRIMER_RIGHT_33_TM=63.282
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=42.308
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=5.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=977
PRIMER_PAIR_34_PENALTY=13.556453
PRIMER_LEFT_34_PENALTY=4.954492
PRIMER_RIGHT_34_PENALTY=8.601961
PRIMER_LEFT_34_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_34_SEQUENCE=TGGAAATACCTCTGCCTTGAACTCT
PRIMER_LEFT_34=579,20
PRIMER_RIGHT_34=1554,25
PRIMER_LEFT_34_TM=55.046
PRIMER_RIGHT_34_TM=63.602
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=44.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=5.7000
PRIMER_RIGHT_34_END_STABILITY=6.1000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=976
PRIMER_PAIR_35_PENALTY=13.616616
PRIMER_LEFT_35_PENALTY=3.098855
PRIMER_RIGHT_35_PENALTY=10.517761
PRIMER_LEFT_35_SEQUENCE=CAGCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_35_SEQUENCE=TGGAAATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_35=577,22
PRIMER_RIGHT_35=1554,26
PRIMER_LEFT_35_TM=58.901
PRIMER_RIGHT_35_TM=64.518
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=42.308
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=5.7000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=978
PRIMER_PAIR_36_PENALTY=14.236594
PRIMER_LEFT_36_PENALTY=4.954492
PRIMER_RIGHT_36_PENALTY=9.282103
PRIMER_LEFT_36_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_36_SEQUENCE=GGAAATACCTCTGCCTTGAACTCTTT
PRIMER_LEFT_36=579,20
PRIMER_RIGHT_36=1553,26
PRIMER_LEFT_36_TM=55.046
PRIMER_RIGHT_36_TM=63.282
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=42.308
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=5.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=975
PRIMER_PAIR_37_PENALTY=15.472253
PRIMER_LEFT_37_PENALTY=4.954492
PRIMER_RIGHT_37_PENALTY=10.517761
PRIMER_LEFT_37_SEQUENCE=GCTGCCATTCAAAATCTTAC
PRIMER_RIGHT_37_SEQUENCE=TGGAAATACCTCTGCCTTGAACTCTT
PRIMER_LEFT_37=579,20
PRIMER_RIGHT_37=1554,26
PRIMER_LEFT_37_TM=55.046
PRIMER_RIGHT_37_TM=64.518
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=42.308
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.7000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=976
=
SEQUENCE_ID=MAGIC.105_4_11309959_11309962_wu-0_rsch-4_84449_131260_TRUE
SEQUENCE_TARGET=950,103
SEQUENCE_TEMPLATE=CTNTTTNCNNAANNNTNNNNNNNNNNNNNANANGNCANNNNNNNNNNNNNNANNNNCAANANANNNNNTTTGATCATGGCCTCGTTCCTCNNNTGAAATATTTTTNNNNNNNNNNNNNNTTNTNANNANAANTNGNNANTNNANNAGNTTNNTTTGANCANNATNNNTNNTNAANNNNATNNNNNNTNNNNNTGACNANTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNTNNNNNNNNNNNATNNANANCNNNNANNANCTANNNCCNNANCNNNTANNNNANTTNNNNNNNNNNANCNANANNNCANNNTNNATNNNAGNTTTGGNNTTGNTNNGCAANANTTCAANATNANNNNNNNNNNNNNNNNNNNNNNANTTTNNTTGATAGNNGNNNNNTTNANNATNAANNACATNANGNAANNNNNCNNNTAANANATNNTANNNNNTAGCNTANGNNANTNNGGTGNTGNNNTTTATNNTNNNACNCNTGNNGNANNNTNNATNTGNTAANANNNTNNNTNNANNGCANGTNNTGANNTTNATTNTTCNANNCTNNNGTAGNTNNNANTTNNTAAGNNNGNCNNTNTATNANNCAANTNTGATNNTNTANTNNNNNNNNNNANNANAANTNCANNNNNNNCNANNNANNNTNNAATNNNANGNNNNTNTNTNTGNNANANNNNNTNTAANAANATNANNNNNGNTNGTNANNAANNANTNNTTNATNNNANGAAANNTTNNNNNGAANCAAACNANNNNNANANTANTNNNNCNNGTNANANNNAANTGATNANANNNGNNANNNNNTNTTTTNNANANNTNCNNACNNNNNNTNTTNNNNTNTTNGACTTNNCNNNNNATGNNNNAANTNGANANCACNNNTNNNACNNANGTCCNTACGCNNNTNCANTNTNGANNNNNGTATNNGNCNNGTNNNNATNACNNNCNANNACNNCNAATTNNTNCTNANNGNNCNANTNNNTNNTNTANTANTTCAAGCNTTCATTCATCATTTGTGNANAGAGATATGGGAAGNNGCACCACCAGNCNTCTCANTCTNCTCTTCANCAGCCATCGNCAAAGTNATTAGGANTNGAATAGTATCCCGCGCCAATCANGCACNACCNGCAACAACAANNNNNNCTTCTTAANANCAACATGTGCGNCATAANAATGGGCTNNTCNNNNNNTTTGTTTTTGCTCTGTTTNNNTAATGAAANTTTAAGTTCATTTGTTTTTCNTTTTTCCATAANTAATCTNTTNTTTTTTGTTGATNAAATAAATATCAANTTNNNNNNNNNNNNNNNNNNNNNTCCTGCAAGATANNNNNNNNNNNNNNNNNNTNGNAAAAANATTATATATAACACNAANGGTGTTATGTAAACTCAACNTAATTCNATCGTAAGAGAGTTTCTCNATATNTTTGGAAAAGGTNAAACNAAACGAAACTAAAATNACTCACCAGTCACCNCAATTGNTATGTACACATAGAGAACANATNGAAGACAATCGAAACACATACTCGANAAANAAANTTAAAGAAAAAACAGAGAAAAAAGAATTTNAAAGAACAGAAGAGAAGATTCTCTGTCTTTGTCACAATAGTCATAAACTCTTGTCTTAAGCTTTCTTTTCCAATCTCCCATATGATCTCGTGCGTGCCCACTTCCCACTTTCTCTCTCTCTTACTGTTNNNNNNTTNTTTTTTTTNATTNAAAAAAAATCATACGAAATAAAACTATTATCACAACCTTACCCTCTTCATTTTTTGTAATGTTCTTCTGTCTGATACATCTCCTTAAAATGTAGGTCGGTGAGACTGNGGCATGTTCTATGGCCACAAACTTAGGTCGGTCGATGATATATATGCGNTATNTATAATGCTCTTCNTGTGTTTACNATTANTCTAGAGGCATATCTCCGGGANTGGACTTGCTGATCGAGAGTGTGAATATGATTAACAAGATTGATCC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_10117207_10117288_kn-0_oy-0_33556_1319644_TRUE
SEQUENCE_TARGET=950,181
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATNAAATGCAANGGAGATAGAGATCNATGCTCTAGAATCTACNAATACTTGNTCAGTTANTAGCTTNCATTTTTATNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_14538534_14538802_sf-2_oy-0_10864_112200_TRUE
SEQUENCE_TARGET=950,368
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATATAATGGAAGTTCGATTAAGNNTNTCGCATACNNGAGGTGGTTGGCTCGGGTNCNNNNNNNNNNNNNNAAAGTTGNATTTAGTATANTTTGTTNNNNNNNNNNNNNNNNNNTACCANGTTATTCNGGATAACTNGATTTCTAANCACNCAGNNNNNNNNNNATAAGAAACTNNNNNNNNNNNNNNNNNNNNNNNGACACCAGTGTTTATTTCAANNNNNNNNNNNNNNNNNNNNNCNNNNGNTTAGCACCAATGTTTATGATAACTGNGTTTTAGTTTCCNANGNGNTNGNCNCNNTACNAGTTAGGANNNNGNTTTTGTAGGAGTGAATGTATTNGAGCNTTTTCGGGCGAAGCTTTGTCTTTCATGCNCGAGGTNGTTAGCTNNGTACCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGGAGCTNTTTTTTNACTNNNNNGAAGNNTNCNTTTNNTNGGTAGANGAGNCTTTTTGCTCGGTAGACGAGTCNNTTTNCTCGGTAGACNAGTGTTTTTGCNAGTTTATGNAAAANATANAANAGAGANTGCATATATATGATTGAGNTGTGCTTGTTANATTATAATNNATAACANTTATCTTAGNGGTTTGGANNTAGAGATGATGACATCTACGACATGGACGATCTGAATCNNTNAGATTNTAAANGTNGTTGTATGGAANNCTANATTTGAGATGTTNGTAGTNNGGAGGTCNCNTCTTTGCGNTAGAANNAGAGANGCAANGATAAANGCNNNNNNNNNNNNNGAGTATGTCAANCAAGCTTAATTAATATGTNAGAANGTAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANGCGNTAGAGNGAGGGGTCCATGAACGATTNTAAGTNATATTCCATAGTNGCTTGNTGGTGTACTNNTTTTTGCTGATTTGGAGAATAGCTGATAACTTTTGNTTNAATNNTTAGGCTTTNNATCNCACCGTTNAGTCTTTANATGGTAGTTCCATGAANGTCTCCTCCANATTTNAGTCANATCNGATATTGCATGAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNCCTTTGTTTTGCTGATTCAGAGANGCNNNGNTAACTTTAGTCTGGACNGTTGANNNNTNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_5929114_5929209_bur-0_mt-0_4770_4009_TRUE
SEQUENCE_TARGET=950,195
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNATTAANNTNGAGNNNNTNATACTNGCNNGATGCNNAAGTTTGNNGGAGCTCCCTTCCTTTATTGGAAAAGNCACTNATCTCANGATTCTNNATNTTNGANATNTCNNATGTCTGGTNGAGTTACCNTCCTTTATNGGAAACCTTCATAAGTTGNCTNAANTNAGNCTGANAGGATGCNAAAAGCTACAAGTTCTTCCGACNAATATCAACTTGGAATTTCTTNATGAACTTGATCTCACAGATTGCATCTTGTTGAAAACATTTCCNNNGATCTCNACAAANATTNAACGTCTNNATCTTANGGGGACTCNNATAGAAGAAGTTNCTTNGTCCNTCANGTCATGGCCTCGCCTTNNGNATTTGCANATGNTATACAGNNAAAACCTNAGTGAATTCTCACATGTTCTTGAGANGATCACNGTGCTNGAATTGAGNGATATAAATATACGNGAAATGACNCCATGGCTCAATAGAATCNCTCNTCTACGTCGATTNANACTCAGNGGATGCNGAAAACTNGTATCACTCCCACAACTTNCNGATTCCCTAANAATTCTNGATGCAGANAATTGTGNCTCTCTTGAGAGACTAGNTTGCTCTTTTAACAATCCAAATATTNNANNTCTCGACTTTACCAACTGNCTCAAACTAGATAAAGAAGCAAGAGANNTTATCATTCAAGCAACGGCTCGNNATTATTCNATCTTACCNAGTAGAGAAGTTCATGAGTACATCANTAANCGAGCCATTGGAANTTCTCTTANNNTGAAGTTGAATNAAAGGGNNNTTCCTACATCCATGANATTTAAGGCTTGCATNGTTTTGGCTGNTAANNNTGNNCNTGAAGCTNNTANNNNNNNNNNNNNNNNNNNNNNNNTGACCNTNATGGAANGGCAGAATGATTTNATCNCTTCTACGTNTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGAGCATNTGTACNCANTCGAAGTTNNAGTTGANGTTGAGGTNACTTCNGATGAGCTTGTCTTTGATTTCNAGCTCAACANTGAGAAATGGGAGATAGNAGAATGTGGGGTACNNGAANTGAAGAANCATGTTCNAANNTTTTCNGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACANTATGAACGTCTNGGCNAGAGCNTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNANACATATCTCTACAGGCNAAAACNAACAATNGAANTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTATTNAAGNANGTGAGTCCACTTGTGGAAAAGGAAAANNNNNNNNNNNNNNNNNNNGAAATTACTTGGATNNTGCATTTANNTGAAGCNACTCTAGGAAGNATCTGAAGTTGCTTGGAAACTNCAAGCACATCGTNATCGTATGGTGTATNGTAGTATNATATCATTTGNTCAANATTCNGCATTNTCTCNA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_20836789_20836848_po-0_sf-2_8539_353547_TRUE
SEQUENCE_TARGET=950,159
SEQUENCE_TEMPLATE=GGAAAATTCCTTCACCGGAACCACCGTGAAAACTTCGTNTTCACGTGGCNTCAATGTCACTGGTAAAGATGTATCTTTTGGGAGATACACTAACTCTCCTGATAGAAAAGNAAAAAAAAAGTGAAGGTTTTAGTATAATCAGAGTAATTTAGACTGATGTTTGAGTTTGTGTACTACCTCTGAGATGNGAATAGACAATGGAATCTCCTGTCCATTCAAAGGCTGCTACTTTATGAAGATAATGAACATCATTGGTTCGGACACAACCAGAAATGGTCCCAGGTTCTTGGTCATGGATTAGGTATCTCTTCTCGTTCTTACACCATCCAGCGCCTTGGCAGTTGAAAACTCCAATAACGCCAGTGAATTCGTTCAGGTTCCATATTTTCAGAAGACTTGCAAAGTATAATACAACAGTTAGTTAAGATTTAAGCTTTCTTCTCTTCCAAGTAAAGGAAAACGAGATACCTTTTATTGTCTCTGACTGGATCACTGAAGAAGCAATCACTGGTTGGTCTTCCGGGAAGCTTTGCTCTGAGTATGGAACCGTCTCGAAGTACTAGCTTTCTTAAAAGGTTGAAGTCATGTTGTCCCGGTTTGTCGCTGTTTNACAAGTTTNAATGGATCTAGAGTTACTTACTTGCAGCTCATTTTACTATGATAATTTGTTTGTCAAATGGTATTGAATCTTGTGATTACCTGACATATATGGCACATCCTCCAACCGCGCGAGCTGCAGCATGATATTCAGCCATCGGATGCAAACTCTGCAATGAGAAACAGATAGATTCATTCACAANTTCAGAAAAGTTTGTTAAAGCAATTCAACGTACATGAAACATGTCCCAGTCCGGCTGCATAAACTCTCCAAGGAAAAGTGTGTTATATGCAACAGAAGCAATGTGGATCGTGTGTGAAGCGGGGTCTCTAGGCCAGAAATCGTCTGATGCTCTTATAACAGCAGTCTTTTTTGCNCTANAGTATCATTACAAGAAGAAATNAGAAGGGANANNNNNNNNNNNNNNNNNNNNNNNNTGAGAANAACGTTGNAGNTCNAGANGCTAACCTGTAGAGACCATCTGTGTTATGACTCATGCAAGAGATAATACCATTGTCAGGGAAGTTTCTTGAAATAGAAGCTTCCAAAGCCTGGTGATACTTCTTTGCAAGTTTGACACGACCACCATGTCCAGCTCCAAGAGTTTCNAGAATGTTTTGGACGTCTACTTTGACACCATCGANCCCAANGGATGCAAGGTANGAGTGAAGNTCNTTATAGAAGCTAAAGACTTTCTCAGGATTCACCAAACCAAGTCCATTCTTAGTTATNCTTTCTANNCATCCACAATTCTCANTGGACATAACTCCTGGTGAAGAAACTGGATACGCNACCTTNGATTCGTAGTGNTCCATACCAGAAACACCTGGTTTGACTCCTCCCCAATATCCNGTTATCGCATGCCAAACATAAACATACTTGAGNGAATTATTACTCTTAATGTCTGTGATAACATGTCCAAGACTCAATGNAGGGTCATCCACCCGGTGGCCTTCTTTGCCATCCTTTTGAAACTTNTGATTNTCCTTGATGTGAGTTAACCGGTTTGCNAAACTGAAGGNAANCANAAAAGATAAAGAGNGGTTTATTNNNNNNNNNNNNNNAACNNNNNNNNNNNNNNNNNNNNNNNNATTACTTACTTGGCAGCATTATCAGCGTTNAATTCTACACTGGTCTCATCCATGCCAACAGATTGCCATCCATCATCAATTATGACAAACTTTGGNGTAACCCCACCAGCTTTGAGGCTANAGAGAATCANTGCAGGTTTGGTTAAGTCACAGTTANTAAAGCCATCTTNAAAAGTAAAGANTCAGGAGNACCTCTCAAGNCCTTGCTTGACGTCTTTGGCTGTGACATTGGTNTAGAATGCATCCCAAGTGCACCAACCAAACCANTTCANCATATCTGGCATCTGAACACAACCAAAACAGGGGAAATTTTCAGAGTTANTTTTGNNATGNNTATAANCAAAGATCANATAAAANGTCTCAATGTTTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.440854
PRIMER_LEFT_0_PENALTY=0.120245
PRIMER_RIGHT_0_PENALTY=0.320610
PRIMER_LEFT_0_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_0_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_0=894,20
PRIMER_RIGHT_0=1568,20
PRIMER_LEFT_0_TM=60.120
PRIMER_RIGHT_0_TM=59.679
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=675
PRIMER_PAIR_1_PENALTY=0.477827
PRIMER_LEFT_1_PENALTY=0.157217
PRIMER_RIGHT_1_PENALTY=0.320610
PRIMER_LEFT_1_SEQUENCE=GTCCGGCTGCATAAACTCTC
PRIMER_RIGHT_1_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_1=848,20
PRIMER_RIGHT_1=1568,20
PRIMER_LEFT_1_TM=59.843
PRIMER_RIGHT_1_TM=59.679
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=721
PRIMER_PAIR_2_PENALTY=0.483243
PRIMER_LEFT_2_PENALTY=0.120245
PRIMER_RIGHT_2_PENALTY=0.362999
PRIMER_LEFT_2_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_2_SEQUENCE=TCTGTTGGCATGGATGAGAC
PRIMER_LEFT_2=894,20
PRIMER_RIGHT_2=1750,20
PRIMER_LEFT_2_TM=60.120
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=857
PRIMER_PAIR_3_PENALTY=0.493696
PRIMER_LEFT_3_PENALTY=0.173087
PRIMER_RIGHT_3_PENALTY=0.320610
PRIMER_LEFT_3_SEQUENCE=GAAGTCATGTTGTCCCGGTT
PRIMER_RIGHT_3_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_3=578,20
PRIMER_RIGHT_3=1568,20
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=59.679
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=991
PRIMER_PAIR_4_PENALTY=0.520216
PRIMER_LEFT_4_PENALTY=0.157217
PRIMER_RIGHT_4_PENALTY=0.362999
PRIMER_LEFT_4_SEQUENCE=GTCCGGCTGCATAAACTCTC
PRIMER_RIGHT_4_SEQUENCE=TCTGTTGGCATGGATGAGAC
PRIMER_LEFT_4=848,20
PRIMER_RIGHT_4=1750,20
PRIMER_LEFT_4_TM=59.843
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=903
PRIMER_PAIR_5_PENALTY=0.544743
PRIMER_LEFT_5_PENALTY=0.118777
PRIMER_RIGHT_5_PENALTY=0.425966
PRIMER_LEFT_5_SEQUENCE=GCCAGTGAATTCGTTCAGGT
PRIMER_RIGHT_5_SEQUENCE=GACATGGTGGTCGTGTCAAA
PRIMER_LEFT_5=358,20
PRIMER_RIGHT_5=1189,20
PRIMER_LEFT_5_TM=60.119
PRIMER_RIGHT_5_TM=60.426
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=832
PRIMER_PAIR_6_PENALTY=0.598734
PRIMER_LEFT_6_PENALTY=0.278124
PRIMER_RIGHT_6_PENALTY=0.320610
PRIMER_LEFT_6_SEQUENCE=ATGCAACAGAAGCAATGTGG
PRIMER_RIGHT_6_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_6=886,20
PRIMER_RIGHT_6=1568,20
PRIMER_LEFT_6_TM=59.722
PRIMER_RIGHT_6_TM=59.679
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=683
PRIMER_PAIR_7_PENALTY=0.599053
PRIMER_LEFT_7_PENALTY=0.173087
PRIMER_RIGHT_7_PENALTY=0.425966
PRIMER_LEFT_7_SEQUENCE=GAAGTCATGTTGTCCCGGTT
PRIMER_RIGHT_7_SEQUENCE=GACATGGTGGTCGTGTCAAA
PRIMER_LEFT_7=578,20
PRIMER_RIGHT_7=1189,20
PRIMER_LEFT_7_TM=59.827
PRIMER_RIGHT_7_TM=60.426
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.9000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=612
PRIMER_PAIR_8_PENALTY=0.641123
PRIMER_LEFT_8_PENALTY=0.278124
PRIMER_RIGHT_8_PENALTY=0.362999
PRIMER_LEFT_8_SEQUENCE=ATGCAACAGAAGCAATGTGG
PRIMER_RIGHT_8_SEQUENCE=TCTGTTGGCATGGATGAGAC
PRIMER_LEFT_8=886,20
PRIMER_RIGHT_8=1750,20
PRIMER_LEFT_8_TM=59.722
PRIMER_RIGHT_8_TM=59.637
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.1000
PRIMER_PAIR_8_COMPL_ANY=8.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=865
PRIMER_PAIR_9_PENALTY=0.678023
PRIMER_LEFT_9_PENALTY=0.118777
PRIMER_RIGHT_9_PENALTY=0.559246
PRIMER_LEFT_9_SEQUENCE=GCCAGTGAATTCGTTCAGGT
PRIMER_RIGHT_9_SEQUENCE=CATGGTGGTCGTGTCAAACT
PRIMER_LEFT_9=358,20
PRIMER_RIGHT_9=1187,20
PRIMER_LEFT_9_TM=60.119
PRIMER_RIGHT_9_TM=59.441
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=830
PRIMER_PAIR_10_PENALTY=0.685930
PRIMER_LEFT_10_PENALTY=0.365320
PRIMER_RIGHT_10_PENALTY=0.320610
PRIMER_LEFT_10_SEQUENCE=ATATGGCACATCCTCCAACC
PRIMER_RIGHT_10_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_10=706,20
PRIMER_RIGHT_10=1568,20
PRIMER_LEFT_10_TM=59.635
PRIMER_RIGHT_10_TM=59.679
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=863
PRIMER_PAIR_11_PENALTY=0.732333
PRIMER_LEFT_11_PENALTY=0.173087
PRIMER_RIGHT_11_PENALTY=0.559246
PRIMER_LEFT_11_SEQUENCE=GAAGTCATGTTGTCCCGGTT
PRIMER_RIGHT_11_SEQUENCE=CATGGTGGTCGTGTCAAACT
PRIMER_LEFT_11=578,20
PRIMER_RIGHT_11=1187,20
PRIMER_LEFT_11_TM=59.827
PRIMER_RIGHT_11_TM=59.441
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=610
PRIMER_PAIR_12_PENALTY=0.791287
PRIMER_LEFT_12_PENALTY=0.365320
PRIMER_RIGHT_12_PENALTY=0.425966
PRIMER_LEFT_12_SEQUENCE=ATATGGCACATCCTCCAACC
PRIMER_RIGHT_12_SEQUENCE=GACATGGTGGTCGTGTCAAA
PRIMER_LEFT_12=706,20
PRIMER_RIGHT_12=1189,20
PRIMER_LEFT_12_TM=59.635
PRIMER_RIGHT_12_TM=60.426
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=484
PRIMER_PAIR_13_PENALTY=0.822910
PRIMER_LEFT_13_PENALTY=0.396944
PRIMER_RIGHT_13_PENALTY=0.425966
PRIMER_LEFT_13_SEQUENCE=CTGTCCATTCAAAGGCTGCT
PRIMER_RIGHT_13_SEQUENCE=GACATGGTGGTCGTGTCAAA
PRIMER_LEFT_13=207,20
PRIMER_RIGHT_13=1189,20
PRIMER_LEFT_13_TM=60.397
PRIMER_RIGHT_13_TM=60.426
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=983
PRIMER_PAIR_14_PENALTY=0.924120
PRIMER_LEFT_14_PENALTY=0.603510
PRIMER_RIGHT_14_PENALTY=0.320610
PRIMER_LEFT_14_SEQUENCE=AGCAATGTGGATCGTGTGTG
PRIMER_RIGHT_14_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_14=896,20
PRIMER_RIGHT_14=1568,20
PRIMER_LEFT_14_TM=60.604
PRIMER_RIGHT_14_TM=59.679
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=673
PRIMER_PAIR_15_PENALTY=0.956190
PRIMER_LEFT_15_PENALTY=0.396944
PRIMER_RIGHT_15_PENALTY=0.559246
PRIMER_LEFT_15_SEQUENCE=CTGTCCATTCAAAGGCTGCT
PRIMER_RIGHT_15_SEQUENCE=CATGGTGGTCGTGTCAAACT
PRIMER_LEFT_15=207,20
PRIMER_RIGHT_15=1187,20
PRIMER_LEFT_15_TM=60.397
PRIMER_RIGHT_15_TM=59.441
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=981
PRIMER_PAIR_16_PENALTY=0.966509
PRIMER_LEFT_16_PENALTY=0.603510
PRIMER_RIGHT_16_PENALTY=0.362999
PRIMER_LEFT_16_SEQUENCE=AGCAATGTGGATCGTGTGTG
PRIMER_RIGHT_16_SEQUENCE=TCTGTTGGCATGGATGAGAC
PRIMER_LEFT_16=896,20
PRIMER_RIGHT_16=1750,20
PRIMER_LEFT_16_TM=60.604
PRIMER_RIGHT_16_TM=59.637
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=6.1000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=855
PRIMER_PAIR_17_PENALTY=0.998795
PRIMER_LEFT_17_PENALTY=0.678186
PRIMER_RIGHT_17_PENALTY=0.320610
PRIMER_LEFT_17_SEQUENCE=GTCATGTTGTCCCGGTTTGT
PRIMER_RIGHT_17_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_17=581,20
PRIMER_RIGHT_17=1568,20
PRIMER_LEFT_17_TM=60.678
PRIMER_RIGHT_17_TM=59.679
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=988
PRIMER_PAIR_18_PENALTY=1.012173
PRIMER_LEFT_18_PENALTY=0.691563
PRIMER_RIGHT_18_PENALTY=0.320610
PRIMER_LEFT_18_SEQUENCE=AAGTCATGTTGTCCCGGTTT
PRIMER_RIGHT_18_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_18=579,20
PRIMER_RIGHT_18=1568,20
PRIMER_LEFT_18_TM=59.308
PRIMER_RIGHT_18_TM=59.679
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=990
PRIMER_PAIR_19_PENALTY=1.059494
PRIMER_LEFT_19_PENALTY=0.118777
PRIMER_RIGHT_19_PENALTY=0.940718
PRIMER_LEFT_19_SEQUENCE=GCCAGTGAATTCGTTCAGGT
PRIMER_RIGHT_19_SEQUENCE=TGGTTTGGTGAATCCTGAGA
PRIMER_LEFT_19=358,20
PRIMER_RIGHT_19=1311,20
PRIMER_LEFT_19_TM=60.119
PRIMER_RIGHT_19_TM=59.059
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=954
PRIMER_PAIR_20_PENALTY=1.060962
PRIMER_LEFT_20_PENALTY=0.120245
PRIMER_RIGHT_20_PENALTY=0.940718
PRIMER_LEFT_20_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_20_SEQUENCE=TGGTTTGGTGAATCCTGAGA
PRIMER_LEFT_20=894,20
PRIMER_RIGHT_20=1311,20
PRIMER_LEFT_20_TM=60.120
PRIMER_RIGHT_20_TM=59.059
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=418
PRIMER_PAIR_21_PENALTY=1.104152
PRIMER_LEFT_21_PENALTY=0.678186
PRIMER_RIGHT_21_PENALTY=0.425966
PRIMER_LEFT_21_SEQUENCE=GTCATGTTGTCCCGGTTTGT
PRIMER_RIGHT_21_SEQUENCE=GACATGGTGGTCGTGTCAAA
PRIMER_LEFT_21=581,20
PRIMER_RIGHT_21=1189,20
PRIMER_LEFT_21_TM=60.678
PRIMER_RIGHT_21_TM=60.426
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=609
PRIMER_PAIR_22_PENALTY=1.113805
PRIMER_LEFT_22_PENALTY=0.173087
PRIMER_RIGHT_22_PENALTY=0.940718
PRIMER_LEFT_22_SEQUENCE=GAAGTCATGTTGTCCCGGTT
PRIMER_RIGHT_22_SEQUENCE=TGGTTTGGTGAATCCTGAGA
PRIMER_LEFT_22=578,20
PRIMER_RIGHT_22=1311,20
PRIMER_LEFT_22_TM=59.827
PRIMER_RIGHT_22_TM=59.059
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.9000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=734
PRIMER_PAIR_23_PENALTY=1.116946
PRIMER_LEFT_23_PENALTY=0.796337
PRIMER_RIGHT_23_PENALTY=0.320610
PRIMER_LEFT_23_SEQUENCE=CAGTCCGGCTGCATAAACTC
PRIMER_RIGHT_23_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_23=846,20
PRIMER_RIGHT_23=1568,20
PRIMER_LEFT_23_TM=60.796
PRIMER_RIGHT_23_TM=59.679
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=723
PRIMER_PAIR_24_PENALTY=1.117530
PRIMER_LEFT_24_PENALTY=0.691563
PRIMER_RIGHT_24_PENALTY=0.425966
PRIMER_LEFT_24_SEQUENCE=AAGTCATGTTGTCCCGGTTT
PRIMER_RIGHT_24_SEQUENCE=GACATGGTGGTCGTGTCAAA
PRIMER_LEFT_24=579,20
PRIMER_RIGHT_24=1189,20
PRIMER_LEFT_24_TM=59.308
PRIMER_RIGHT_24_TM=60.426
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=611
PRIMER_PAIR_25_PENALTY=1.132456
PRIMER_LEFT_25_PENALTY=0.811846
PRIMER_RIGHT_25_PENALTY=0.320610
PRIMER_LEFT_25_SEQUENCE=AGTCATGTTGTCCCGGTTTG
PRIMER_RIGHT_25_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_25=580,20
PRIMER_RIGHT_25=1568,20
PRIMER_LEFT_25_TM=60.812
PRIMER_RIGHT_25_TM=59.679
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=989
PRIMER_PAIR_26_PENALTY=1.141783
PRIMER_LEFT_26_PENALTY=0.120245
PRIMER_RIGHT_26_PENALTY=1.021538
PRIMER_LEFT_26_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_26_SEQUENCE=CCGGTTAACTCACATCAAGGA
PRIMER_LEFT_26=894,20
PRIMER_RIGHT_26=1602,21
PRIMER_LEFT_26_TM=60.120
PRIMER_RIGHT_26_TM=59.978
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=709
PRIMER_PAIR_27_PENALTY=1.159335
PRIMER_LEFT_27_PENALTY=0.796337
PRIMER_RIGHT_27_PENALTY=0.362999
PRIMER_LEFT_27_SEQUENCE=CAGTCCGGCTGCATAAACTC
PRIMER_RIGHT_27_SEQUENCE=TCTGTTGGCATGGATGAGAC
PRIMER_LEFT_27=846,20
PRIMER_RIGHT_27=1750,20
PRIMER_LEFT_27_TM=60.796
PRIMER_RIGHT_27_TM=59.637
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.1000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=905
PRIMER_PAIR_28_PENALTY=1.171158
PRIMER_LEFT_28_PENALTY=0.120245
PRIMER_RIGHT_28_PENALTY=1.050914
PRIMER_LEFT_28_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_28_SEQUENCE=ATCTGTTGGCATGGATGAGAC
PRIMER_LEFT_28=894,20
PRIMER_RIGHT_28=1751,21
PRIMER_LEFT_28_TM=60.120
PRIMER_RIGHT_28_TM=59.949
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=858
PRIMER_PAIR_29_PENALTY=1.178756
PRIMER_LEFT_29_PENALTY=0.157217
PRIMER_RIGHT_29_PENALTY=1.021538
PRIMER_LEFT_29_SEQUENCE=GTCCGGCTGCATAAACTCTC
PRIMER_RIGHT_29_SEQUENCE=CCGGTTAACTCACATCAAGGA
PRIMER_LEFT_29=848,20
PRIMER_RIGHT_29=1602,21
PRIMER_LEFT_29_TM=59.843
PRIMER_RIGHT_29_TM=59.978
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=755
PRIMER_PAIR_30_PENALTY=1.208131
PRIMER_LEFT_30_PENALTY=0.157217
PRIMER_RIGHT_30_PENALTY=1.050914
PRIMER_LEFT_30_SEQUENCE=GTCCGGCTGCATAAACTCTC
PRIMER_RIGHT_30_SEQUENCE=ATCTGTTGGCATGGATGAGAC
PRIMER_LEFT_30=848,20
PRIMER_RIGHT_30=1751,21
PRIMER_LEFT_30_TM=59.843
PRIMER_RIGHT_30_TM=59.949
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=904
PRIMER_PAIR_31_PENALTY=1.218842
PRIMER_LEFT_31_PENALTY=0.278124
PRIMER_RIGHT_31_PENALTY=0.940718
PRIMER_LEFT_31_SEQUENCE=ATGCAACAGAAGCAATGTGG
PRIMER_RIGHT_31_SEQUENCE=TGGTTTGGTGAATCCTGAGA
PRIMER_LEFT_31=886,20
PRIMER_RIGHT_31=1311,20
PRIMER_LEFT_31_TM=59.722
PRIMER_RIGHT_31_TM=59.059
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=426
PRIMER_PAIR_32_PENALTY=1.237432
PRIMER_LEFT_32_PENALTY=0.678186
PRIMER_RIGHT_32_PENALTY=0.559246
PRIMER_LEFT_32_SEQUENCE=GTCATGTTGTCCCGGTTTGT
PRIMER_RIGHT_32_SEQUENCE=CATGGTGGTCGTGTCAAACT
PRIMER_LEFT_32=581,20
PRIMER_RIGHT_32=1187,20
PRIMER_LEFT_32_TM=60.678
PRIMER_RIGHT_32_TM=59.441
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=607
PRIMER_PAIR_33_PENALTY=1.245307
PRIMER_LEFT_33_PENALTY=0.120245
PRIMER_RIGHT_33_PENALTY=1.125062
PRIMER_LEFT_33_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_33_SEQUENCE=GCGTATCCAGTTTCTTCACCA
PRIMER_LEFT_33=894,20
PRIMER_RIGHT_33=1388,21
PRIMER_LEFT_33_TM=60.120
PRIMER_RIGHT_33_TM=60.125
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=495
PRIMER_PAIR_34_PENALTY=1.250809
PRIMER_LEFT_34_PENALTY=0.691563
PRIMER_RIGHT_34_PENALTY=0.559246
PRIMER_LEFT_34_SEQUENCE=AAGTCATGTTGTCCCGGTTT
PRIMER_RIGHT_34_SEQUENCE=CATGGTGGTCGTGTCAAACT
PRIMER_LEFT_34=579,20
PRIMER_RIGHT_34=1187,20
PRIMER_LEFT_34_TM=59.308
PRIMER_RIGHT_34_TM=59.441
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=609
PRIMER_PAIR_35_PENALTY=1.258358
PRIMER_LEFT_35_PENALTY=0.120245
PRIMER_RIGHT_35_PENALTY=1.138114
PRIMER_LEFT_35_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_35_SEQUENCE=GGATATTGGGGAGGAGTCAAA
PRIMER_LEFT_35=894,20
PRIMER_RIGHT_35=1448,21
PRIMER_LEFT_35_TM=60.120
PRIMER_RIGHT_35_TM=60.138
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.3000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=555
PRIMER_PAIR_36_PENALTY=1.268610
PRIMER_LEFT_36_PENALTY=0.842644
PRIMER_RIGHT_36_PENALTY=0.425966
PRIMER_LEFT_36_SEQUENCE=TGAAAACTCCAATAACGCCA
PRIMER_RIGHT_36_SEQUENCE=GACATGGTGGTCGTGTCAAA
PRIMER_LEFT_36=342,20
PRIMER_RIGHT_36=1189,20
PRIMER_LEFT_36_TM=59.157
PRIMER_RIGHT_36_TM=60.426
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=11.7000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=848
PRIMER_PAIR_37_PENALTY=1.282280
PRIMER_LEFT_37_PENALTY=0.157217
PRIMER_RIGHT_37_PENALTY=1.125062
PRIMER_LEFT_37_SEQUENCE=GTCCGGCTGCATAAACTCTC
PRIMER_RIGHT_37_SEQUENCE=GCGTATCCAGTTTCTTCACCA
PRIMER_LEFT_37=848,20
PRIMER_RIGHT_37=1388,21
PRIMER_LEFT_37_TM=59.843
PRIMER_RIGHT_37_TM=60.125
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=541
PRIMER_PAIR_38_PENALTY=1.284362
PRIMER_LEFT_38_PENALTY=0.120245
PRIMER_RIGHT_38_PENALTY=1.164117
PRIMER_LEFT_38_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_38_SEQUENCE=TATTGGGGAGGAGTCAAACC
PRIMER_LEFT_38=894,20
PRIMER_RIGHT_38=1445,20
PRIMER_LEFT_38_TM=60.120
PRIMER_RIGHT_38_TM=58.836
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=552
PRIMER_PAIR_39_PENALTY=1.295331
PRIMER_LEFT_39_PENALTY=0.157217
PRIMER_RIGHT_39_PENALTY=1.138114
PRIMER_LEFT_39_SEQUENCE=GTCCGGCTGCATAAACTCTC
PRIMER_RIGHT_39_SEQUENCE=GGATATTGGGGAGGAGTCAAA
PRIMER_LEFT_39=848,20
PRIMER_RIGHT_39=1448,21
PRIMER_LEFT_39_TM=59.843
PRIMER_RIGHT_39_TM=60.138
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=601
PRIMER_PAIR_40_PENALTY=1.297075
PRIMER_LEFT_40_PENALTY=0.118777
PRIMER_RIGHT_40_PENALTY=1.178298
PRIMER_LEFT_40_SEQUENCE=GCCAGTGAATTCGTTCAGGT
PRIMER_RIGHT_40_SEQUENCE=GAATGGACTTGGTTTGGTGAA
PRIMER_LEFT_40=358,20
PRIMER_RIGHT_40=1320,21
PRIMER_LEFT_40_TM=60.119
PRIMER_RIGHT_40_TM=59.822
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=963
PRIMER_PAIR_41_PENALTY=1.298149
PRIMER_LEFT_41_PENALTY=0.173087
PRIMER_RIGHT_41_PENALTY=1.125062
PRIMER_LEFT_41_SEQUENCE=GAAGTCATGTTGTCCCGGTT
PRIMER_RIGHT_41_SEQUENCE=GCGTATCCAGTTTCTTCACCA
PRIMER_LEFT_41=578,20
PRIMER_RIGHT_41=1388,21
PRIMER_LEFT_41_TM=59.827
PRIMER_RIGHT_41_TM=60.125
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=811
PRIMER_PAIR_42_PENALTY=1.298543
PRIMER_LEFT_42_PENALTY=0.120245
PRIMER_RIGHT_42_PENALTY=1.178298
PRIMER_LEFT_42_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_42_SEQUENCE=GAATGGACTTGGTTTGGTGAA
PRIMER_LEFT_42=894,20
PRIMER_RIGHT_42=1320,21
PRIMER_LEFT_42_TM=60.120
PRIMER_RIGHT_42_TM=59.822
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=427
PRIMER_PAIR_43_PENALTY=1.299662
PRIMER_LEFT_43_PENALTY=0.278124
PRIMER_RIGHT_43_PENALTY=1.021538
PRIMER_LEFT_43_SEQUENCE=ATGCAACAGAAGCAATGTGG
PRIMER_RIGHT_43_SEQUENCE=CCGGTTAACTCACATCAAGGA
PRIMER_LEFT_43=886,20
PRIMER_RIGHT_43=1602,21
PRIMER_LEFT_43_TM=59.722
PRIMER_RIGHT_43_TM=59.978
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=717
PRIMER_PAIR_44_PENALTY=1.306038
PRIMER_LEFT_44_PENALTY=0.365320
PRIMER_RIGHT_44_PENALTY=0.940718
PRIMER_LEFT_44_SEQUENCE=ATATGGCACATCCTCCAACC
PRIMER_RIGHT_44_SEQUENCE=TGGTTTGGTGAATCCTGAGA
PRIMER_LEFT_44=706,20
PRIMER_RIGHT_44=1311,20
PRIMER_LEFT_44_TM=59.635
PRIMER_RIGHT_44_TM=59.059
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=606
PRIMER_PAIR_45_PENALTY=1.306783
PRIMER_LEFT_45_PENALTY=0.986173
PRIMER_RIGHT_45_PENALTY=0.320610
PRIMER_LEFT_45_SEQUENCE=AAGCAATGTGGATCGTGTGT
PRIMER_RIGHT_45_SEQUENCE=CAAAAGGATGGCAAAGAAGG
PRIMER_LEFT_45=895,20
PRIMER_RIGHT_45=1568,20
PRIMER_LEFT_45_TM=59.014
PRIMER_RIGHT_45_TM=59.679
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=674
PRIMER_PAIR_46_PENALTY=1.311201
PRIMER_LEFT_46_PENALTY=0.173087
PRIMER_RIGHT_46_PENALTY=1.138114
PRIMER_LEFT_46_SEQUENCE=GAAGTCATGTTGTCCCGGTT
PRIMER_RIGHT_46_SEQUENCE=GGATATTGGGGAGGAGTCAAA
PRIMER_LEFT_46=578,20
PRIMER_RIGHT_46=1448,21
PRIMER_LEFT_46_TM=59.827
PRIMER_RIGHT_46_TM=60.138
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=871
PRIMER_PAIR_47_PENALTY=1.321335
PRIMER_LEFT_47_PENALTY=0.157217
PRIMER_RIGHT_47_PENALTY=1.164117
PRIMER_LEFT_47_SEQUENCE=GTCCGGCTGCATAAACTCTC
PRIMER_RIGHT_47_SEQUENCE=TATTGGGGAGGAGTCAAACC
PRIMER_LEFT_47=848,20
PRIMER_RIGHT_47=1445,20
PRIMER_LEFT_47_TM=59.843
PRIMER_RIGHT_47_TM=58.836
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=598
PRIMER_PAIR_48_PENALTY=1.329038
PRIMER_LEFT_48_PENALTY=0.278124
PRIMER_RIGHT_48_PENALTY=1.050914
PRIMER_LEFT_48_SEQUENCE=ATGCAACAGAAGCAATGTGG
PRIMER_RIGHT_48_SEQUENCE=ATCTGTTGGCATGGATGAGAC
PRIMER_LEFT_48=886,20
PRIMER_RIGHT_48=1751,21
PRIMER_LEFT_48_TM=59.722
PRIMER_RIGHT_48_TM=59.949
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.1000
PRIMER_PAIR_48_COMPL_ANY=8.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=866
PRIMER_PAIR_49_PENALTY=1.333106
PRIMER_LEFT_49_PENALTY=0.120245
PRIMER_RIGHT_49_PENALTY=1.212862
PRIMER_LEFT_49_SEQUENCE=GAAGCAATGTGGATCGTGTG
PRIMER_RIGHT_49_SEQUENCE=GGATATTGGGGAGGAGTCAA
PRIMER_LEFT_49=894,20
PRIMER_RIGHT_49=1448,20
PRIMER_LEFT_49_TM=60.120
PRIMER_RIGHT_49_TM=58.787
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=555
=
SEQUENCE_ID=MAGIC.426_3_11103633_11103966_wil-2_zu-0_622314_230889_TRUE
SEQUENCE_TARGET=950,433
SEQUENCE_TEMPLATE=CTTACTTTTAATGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGANTAGCATACCTTAGTTTCAAGAAGATGTAATCGAGGTCTGCTTTCATCGACTTCAAAAGACGGGTACTTCTAGCGAATTCCAATGATACATCACTAAAGCAATTCTCTGCAAATTCGTTGTAATGGGAGAGAACTGCATTGCTATCCTGTAGCCTCCCCAATCTANNNNNNNNNNNNAACATANCAAGTTCTTCANATAAACCAAGTCACCAAGANATAAAAAGATGAAANNGATTCAAACAAAAGACTTGTCACCACCTATNAACNCCGTAACNATTAACCNCAACCGCATATANNGAACTCACNTCGGTNGNGTCTAGTGTGATCAATGTNAANANNCTANATTAGATTCCACCAGTNTTTCNTCAATTNTTATCACTTNNNNNNNNNNGATTAAGCTTNAGAGAGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNAGGACAAAAATGAGAAACTTNAAAAGCTTACATACATGAGATGCTGCAAATGTCTAAGAGAATTCANATCNTCTACTTTAACAAGAGTTTTGAATTCNTNAGACACTTCTTGTGAAGCTGCTCTTATGGTTTCTTCCATCNGACACTTCTTATGAACNTGCTCTNATNGNNNTNTTCNACCTAAATGACAAAGANCATAANAGNCAAAGTAATGTCTTAAATCAGACAGANNCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAANATTCTACATTACCCAGAAAGCATNAGAGNTANAGATTCAAAATCCTCAACTANNANAACGAAATCACCTNTTCGAGAATTTCTTCAAGAGATCTAAANCAAACGTANTTTGAAACAANTNAATCAANATCNCAAAGAGGNAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACAGAATCAGCAGNCCTTGTGGNTGAGACTTGATTCGGTAATNTCGGACTTNGACATTTTTCGATTATAAAAGNTTTANNTTNCAGTTTTNTACNTAAAGAGANAAAGAGAGNGTTAATTTTNTTAAAAGGCCGAGATCTATGAAGAATTAAATNGNTGATGGGCCTGCTTTTGGNGTATAAAGCAACATNTNTAGCCACTATGANAGTAGACNCACTGAANACNNNANAGCAACGTACTGATNTCAAATNAAANNTTNCAGAAGTTGGGAAGCCNNNNNNNNNNNNNNNNANAAAANAAAAAANCNTGACATTTGAAGACCANNCCAANTATGAATTTTANNNCNNTNTGAAANAACCANNATNATGTNTGGNGTATTGNGANGTTAGNNANNTTNTNGGNGNTTTTATAGATATANNNATATTTTNGCCNAANAATTANAAAANAGACNNATTTNACTTNNNNTTATAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCNCNNGATTTTTCNNACCAACAATCTTCTNCTTCTNANCNCCGACTCAACANNNTATCAAAATCNTAATTTNCAGNTCCAACAAAATNGTTCCTTCACCTCNNACAANNNATACAACATNTTNTNCACCANNCTCNTCCNNTAAAATCNGATANATCTANTTAAAANAGNGTTGATGCAAANAATCTAAACTNNATNNGNATAATGTNTANNNNANCCACNGATTCTAATTTTAGACNCANATNAAATCAATTCATTCTAAACCAANTTTANTTGNTAGTGTNGACNAANCTTCCCATCACGGTTTGTAACATGAGATCTTGAAAGCAGNCAGTCAAAGGCTGGTCGATTANGCCTTCNAACTGNNATTTTNAAATCTCAAATTGGANNTTNGAAATCAGATANATATAACNTTTATGGTCGNATTTTGGNGNAANAANAAAAAAAATGATNGATTTAAAGAGACAAAGAGNTGANTNATTATGGTAGTCATTGNNCAATANCANTTANGAGTATTCTTCTNTATGGTTTGTGTGTGTTTCNAACCTGNGTTTTGGATTCNNTNTGGTTGTGTGGGTGAGAGATAATNCNACNNNAAAAAATTAANAAAGAGAGAANANNAAATTATTAGGTTTNTNTN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_10908235_10908277_bur-0_wu-0_164_7676585_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=TGCAATAGCATGTCGAAATCGAGCGATGGTGTGCAAGCACGTCCTTCGCCGCAAGATTTGCTTCTTCGAAACGCAGTGGCNGCATCTTGAACGAGCATTAGGTCAAGAACTTGCTCAGTNGTTCTGTCCTTCCAGTTGTGAATAGAGCTATTTCTAGGTGACGTNGCTGCTGAGTTTGGGAGGCTGCAGCTCACAAACTTCGTCTTCATTTGATGATCNNNNNNNNNGTCATCGATGTCTCTCGGACGTGAGACTATCATATCGAAGCTCCCATTCCTNGACAATAGCGATTGCGGATCAACCGAGATCCGCCTCTCGGATACGGATTGCATACTCGTCTTCTTGATAAGAATCTTGGGCTCGTCTCCACCACCTAGAACCTCAAAGTCATGCCAGTCCTCGAGACTACTATCGTCCCTGAAGCTGAGAACATCTGTNNCCATTTGTTNCTCTTTGGCCTGNAANNGAATGAGGACTAATAAGAAAATGAAAATATTGGCAAAANGNCTAANACAGATTGATTGATACTTAAATACGGAGCAGGATGGGTTGCACGTCGGTGNCTACNTTAGTCATGACTTGCACGTTTAATATAATATAACCCAAAGTGTTGTTGACTTCATCTNCTAAAAAANGAAAAATGNNTCATNNTTTTTTTATTNGTTAANTATCNCANTATCGTTGACATGAGACCATNCCCANGATCAAGACAAGATCAAAAAGATAGATCTGCCTGCACGAACTACCTAATTATAATATTACCAAAGNATTTGGAGGGTNGTAATCTGCATCAGTGAAGACCATAAGTAGAAGCCATAAATCTTGAATACNTCTNCNNNTNNATGNACNTNGTNCTNTATNNNNTACNTNNTACTTGGNNANTTNTTTNTNNTTGGTNACNTNTNNTTNTCNTTGNTNACTNTNCNCGNNANACTTNGNTATANNNCTTNNTNATANNNCTTNTNNNTNNNTCTNNTATTTTTNNNTTNNNNTNNNTTTTNCNNNNNNNNNNNNNNNNNCTTTGAATTTTNNTGTGTCTTTTNCTACAAATATTTCTTCACTTTCATTGAAATTCATCNCCTTCAATGGATGCACNTACGTCCGTTAGTCATNNNTGGCTTTAGGCCTCATATNTATTCTAGNTGCTCTAGTATATCCCTCNAAAATGGACGGTGAGAAACAATACCAATTTTTNATCCCAAGGGATTTGAACCGGTTATGTTTGAATAACATAGNGATTTGAACGTCAAATCAACAANTTACGAAACGATTAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGCTAAAACATTTTTGAANGTTTTCAAATATGGATTTGATCGACAAANATAGAAGTCATATACTCCTTGATTTTTCCTTGGGGATTGGTGNATCCTTCATTTNCATTGTAGGNGACGNTCATCATNCGTGNAAGATCAATGGTTGCCTTAAGAAGAAAATGTGGCACGCCAGTTGTTGNCAAGAACTCCTCATTTAGTGTCTTATTGGCANCTNCAACNATTTTANNAAGCTCCCTAAAAGNTTCCTCTTTGGTAACCCCATATTGCTTCATATANCAGTTNACCGCGTTCGNGANATATCCTCTACTCATGTCNTCCTACTCAATATTTGNGTAAGTNGGTAAGATTTACAAGAAAGCAGTTATACGCGAGATGTAAAGAAANNATTTATGTATTTGTACCTCATAACCCATNAAATCATTNATAAGACGACCTTTGATAGACAAATATTGGACGAGTTTTGGTCTGGATTTCAGCCNNTCAAAAGCTTCCTTTGTNGCCATCTTTCCNATAGACATGAAAATNCCTGCCAAGGACGCATACACTGTGACCTCCACCTCACCAACCTCCATATACTNCTCAAAGCTAGGAATGTGATCNGCTTGTGNCCATTTTTCGAGATCAATGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.350503
PRIMER_LEFT_0_PENALTY=0.083883
PRIMER_RIGHT_0_PENALTY=0.266620
PRIMER_LEFT_0_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_0_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_0=529,20
PRIMER_RIGHT_0=1500,20
PRIMER_LEFT_0_TM=59.916
PRIMER_RIGHT_0_TM=59.733
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=972
PRIMER_PAIR_1_PENALTY=0.541950
PRIMER_LEFT_1_PENALTY=0.285121
PRIMER_RIGHT_1_PENALTY=0.256829
PRIMER_LEFT_1_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_1_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_1=727,20
PRIMER_RIGHT_1=1582,20
PRIMER_LEFT_1_TM=60.285
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=0.551741
PRIMER_LEFT_2_PENALTY=0.285121
PRIMER_RIGHT_2_PENALTY=0.266620
PRIMER_LEFT_2_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_2_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_2=727,20
PRIMER_RIGHT_2=1500,20
PRIMER_LEFT_2_TM=60.285
PRIMER_RIGHT_2_TM=59.733
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=774
PRIMER_PAIR_3_PENALTY=0.566115
PRIMER_LEFT_3_PENALTY=0.083883
PRIMER_RIGHT_3_PENALTY=0.482233
PRIMER_LEFT_3_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_3_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_3=529,20
PRIMER_RIGHT_3=1501,20
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=60.482
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=973
PRIMER_PAIR_4_PENALTY=0.767353
PRIMER_LEFT_4_PENALTY=0.285121
PRIMER_RIGHT_4_PENALTY=0.482233
PRIMER_LEFT_4_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_4_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_4=727,20
PRIMER_RIGHT_4=1501,20
PRIMER_LEFT_4_TM=60.285
PRIMER_RIGHT_4_TM=60.482
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=775
PRIMER_PAIR_5_PENALTY=0.769116
PRIMER_LEFT_5_PENALTY=0.512286
PRIMER_RIGHT_5_PENALTY=0.256829
PRIMER_LEFT_5_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_5_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_5=730,20
PRIMER_RIGHT_5=1582,20
PRIMER_LEFT_5_TM=59.488
PRIMER_RIGHT_5_TM=60.257
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.5000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=853
PRIMER_PAIR_6_PENALTY=0.778907
PRIMER_LEFT_6_PENALTY=0.512286
PRIMER_RIGHT_6_PENALTY=0.266620
PRIMER_LEFT_6_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_6_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_6=730,20
PRIMER_RIGHT_6=1500,20
PRIMER_LEFT_6_TM=59.488
PRIMER_RIGHT_6_TM=59.733
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=771
PRIMER_PAIR_7_PENALTY=0.979374
PRIMER_LEFT_7_PENALTY=0.712754
PRIMER_RIGHT_7_PENALTY=0.266620
PRIMER_LEFT_7_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_7_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_7=531,20
PRIMER_RIGHT_7=1500,20
PRIMER_LEFT_7_TM=60.713
PRIMER_RIGHT_7_TM=59.733
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=970
PRIMER_PAIR_8_PENALTY=0.979374
PRIMER_LEFT_8_PENALTY=0.712754
PRIMER_RIGHT_8_PENALTY=0.266620
PRIMER_LEFT_8_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_8_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_8=530,20
PRIMER_RIGHT_8=1500,20
PRIMER_LEFT_8_TM=60.713
PRIMER_RIGHT_8_TM=59.733
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=0.994519
PRIMER_LEFT_9_PENALTY=0.512286
PRIMER_RIGHT_9_PENALTY=0.482233
PRIMER_LEFT_9_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_9_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_9=730,20
PRIMER_RIGHT_9=1501,20
PRIMER_LEFT_9_TM=59.488
PRIMER_RIGHT_9_TM=60.482
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.5000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=772
PRIMER_PAIR_10_PENALTY=1.194986
PRIMER_LEFT_10_PENALTY=0.712754
PRIMER_RIGHT_10_PENALTY=0.482233
PRIMER_LEFT_10_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_10_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_10=531,20
PRIMER_RIGHT_10=1501,20
PRIMER_LEFT_10_TM=60.713
PRIMER_RIGHT_10_TM=60.482
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=971
PRIMER_PAIR_11_PENALTY=1.194986
PRIMER_LEFT_11_PENALTY=0.712754
PRIMER_RIGHT_11_PENALTY=0.482233
PRIMER_LEFT_11_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_11_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_11=530,20
PRIMER_RIGHT_11=1501,20
PRIMER_LEFT_11_TM=60.713
PRIMER_RIGHT_11_TM=60.482
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=1.227016
PRIMER_LEFT_12_PENALTY=0.083883
PRIMER_RIGHT_12_PENALTY=1.143133
PRIMER_LEFT_12_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_12_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_12=529,20
PRIMER_RIGHT_12=1496,20
PRIMER_LEFT_12_TM=59.916
PRIMER_RIGHT_12_TM=61.143
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=968
PRIMER_PAIR_13_PENALTY=1.227016
PRIMER_LEFT_13_PENALTY=0.083883
PRIMER_RIGHT_13_PENALTY=1.143133
PRIMER_LEFT_13_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_13_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_13=529,20
PRIMER_RIGHT_13=1497,20
PRIMER_LEFT_13_TM=59.916
PRIMER_RIGHT_13_TM=61.143
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=969
PRIMER_PAIR_14_PENALTY=1.239333
PRIMER_LEFT_14_PENALTY=0.083883
PRIMER_RIGHT_14_PENALTY=1.155450
PRIMER_LEFT_14_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_14_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_14=529,20
PRIMER_RIGHT_14=1186,21
PRIMER_LEFT_14_TM=59.916
PRIMER_RIGHT_14_TM=59.845
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=658
PRIMER_PAIR_15_PENALTY=1.239333
PRIMER_LEFT_15_PENALTY=0.083883
PRIMER_RIGHT_15_PENALTY=1.155450
PRIMER_LEFT_15_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_15_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_15=529,20
PRIMER_RIGHT_15=1187,21
PRIMER_LEFT_15_TM=59.916
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=659
PRIMER_PAIR_16_PENALTY=1.252490
PRIMER_LEFT_16_PENALTY=0.995660
PRIMER_RIGHT_16_PENALTY=0.256829
PRIMER_LEFT_16_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_16_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_16=728,20
PRIMER_RIGHT_16=1582,20
PRIMER_LEFT_16_TM=60.996
PRIMER_RIGHT_16_TM=60.257
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=855
PRIMER_PAIR_17_PENALTY=1.262281
PRIMER_LEFT_17_PENALTY=0.995660
PRIMER_RIGHT_17_PENALTY=0.266620
PRIMER_LEFT_17_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_17_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_17=728,20
PRIMER_RIGHT_17=1500,20
PRIMER_LEFT_17_TM=60.996
PRIMER_RIGHT_17_TM=59.733
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=773
PRIMER_PAIR_18_PENALTY=1.428253
PRIMER_LEFT_18_PENALTY=0.285121
PRIMER_RIGHT_18_PENALTY=1.143133
PRIMER_LEFT_18_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_18_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_18=727,20
PRIMER_RIGHT_18=1496,20
PRIMER_LEFT_18_TM=60.285
PRIMER_RIGHT_18_TM=61.143
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=770
PRIMER_PAIR_19_PENALTY=1.428253
PRIMER_LEFT_19_PENALTY=0.285121
PRIMER_RIGHT_19_PENALTY=1.143133
PRIMER_LEFT_19_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_19_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_19=727,20
PRIMER_RIGHT_19=1497,20
PRIMER_LEFT_19_TM=60.285
PRIMER_RIGHT_19_TM=61.143
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=771
PRIMER_PAIR_20_PENALTY=1.440571
PRIMER_LEFT_20_PENALTY=0.285121
PRIMER_RIGHT_20_PENALTY=1.155450
PRIMER_LEFT_20_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_20_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_20=727,20
PRIMER_RIGHT_20=1186,21
PRIMER_LEFT_20_TM=60.285
PRIMER_RIGHT_20_TM=59.845
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=460
PRIMER_PAIR_21_PENALTY=1.440571
PRIMER_LEFT_21_PENALTY=0.285121
PRIMER_RIGHT_21_PENALTY=1.155450
PRIMER_LEFT_21_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_21_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_21=727,20
PRIMER_RIGHT_21=1187,21
PRIMER_LEFT_21_TM=60.285
PRIMER_RIGHT_21_TM=59.845
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=461
PRIMER_PAIR_22_PENALTY=1.465064
PRIMER_LEFT_22_PENALTY=0.083883
PRIMER_RIGHT_22_PENALTY=1.381181
PRIMER_LEFT_22_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_22_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_22=529,20
PRIMER_RIGHT_22=1495,20
PRIMER_LEFT_22_TM=59.916
PRIMER_RIGHT_22_TM=58.619
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=967
PRIMER_PAIR_23_PENALTY=1.477893
PRIMER_LEFT_23_PENALTY=0.995660
PRIMER_RIGHT_23_PENALTY=0.482233
PRIMER_LEFT_23_SEQUENCE=TCTGCCTGCACGAACTACCT
PRIMER_RIGHT_23_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_23=728,20
PRIMER_RIGHT_23=1501,20
PRIMER_LEFT_23_TM=60.996
PRIMER_RIGHT_23_TM=60.482
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=774
PRIMER_PAIR_24_PENALTY=1.581829
PRIMER_LEFT_24_PENALTY=0.426379
PRIMER_RIGHT_24_PENALTY=1.155450
PRIMER_LEFT_24_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_24_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_24=361,20
PRIMER_RIGHT_24=1186,21
PRIMER_LEFT_24_TM=59.574
PRIMER_RIGHT_24_TM=59.845
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=826
PRIMER_PAIR_25_PENALTY=1.581829
PRIMER_LEFT_25_PENALTY=0.426379
PRIMER_RIGHT_25_PENALTY=1.155450
PRIMER_LEFT_25_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_25_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_25=361,20
PRIMER_RIGHT_25=1187,21
PRIMER_LEFT_25_TM=59.574
PRIMER_RIGHT_25_TM=59.845
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=827
PRIMER_PAIR_26_PENALTY=1.637322
PRIMER_LEFT_26_PENALTY=0.083883
PRIMER_RIGHT_26_PENALTY=1.553439
PRIMER_LEFT_26_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_26_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_26=529,20
PRIMER_RIGHT_26=1495,21
PRIMER_LEFT_26_TM=59.916
PRIMER_RIGHT_26_TM=59.447
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=967
PRIMER_PAIR_27_PENALTY=1.655419
PRIMER_LEFT_27_PENALTY=0.512286
PRIMER_RIGHT_27_PENALTY=1.143133
PRIMER_LEFT_27_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_27_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_27=730,20
PRIMER_RIGHT_27=1496,20
PRIMER_LEFT_27_TM=59.488
PRIMER_RIGHT_27_TM=61.143
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.5000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=767
PRIMER_PAIR_28_PENALTY=1.655419
PRIMER_LEFT_28_PENALTY=0.512286
PRIMER_RIGHT_28_PENALTY=1.143133
PRIMER_LEFT_28_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_28_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_28=730,20
PRIMER_RIGHT_28=1497,20
PRIMER_LEFT_28_TM=59.488
PRIMER_RIGHT_28_TM=61.143
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.5000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=768
PRIMER_PAIR_29_PENALTY=1.666302
PRIMER_LEFT_29_PENALTY=0.285121
PRIMER_RIGHT_29_PENALTY=1.381181
PRIMER_LEFT_29_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_29_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_29=727,20
PRIMER_RIGHT_29=1495,20
PRIMER_LEFT_29_TM=60.285
PRIMER_RIGHT_29_TM=58.619
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=769
PRIMER_PAIR_30_PENALTY=1.667737
PRIMER_LEFT_30_PENALTY=0.512286
PRIMER_RIGHT_30_PENALTY=1.155450
PRIMER_LEFT_30_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_30_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_30=730,20
PRIMER_RIGHT_30=1186,21
PRIMER_LEFT_30_TM=59.488
PRIMER_RIGHT_30_TM=59.845
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.5000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=457
PRIMER_PAIR_31_PENALTY=1.667737
PRIMER_LEFT_31_PENALTY=0.512286
PRIMER_RIGHT_31_PENALTY=1.155450
PRIMER_LEFT_31_SEQUENCE=TGCCTGCACGAACTACCTAA
PRIMER_RIGHT_31_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_31=730,20
PRIMER_RIGHT_31=1187,21
PRIMER_LEFT_31_TM=59.488
PRIMER_RIGHT_31_TM=59.845
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=458
PRIMER_PAIR_32_PENALTY=1.669226
PRIMER_LEFT_32_PENALTY=0.513776
PRIMER_RIGHT_32_PENALTY=1.155450
PRIMER_LEFT_32_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_32_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_32=257,20
PRIMER_RIGHT_32=1186,21
PRIMER_LEFT_32_TM=59.486
PRIMER_RIGHT_32_TM=59.845
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=930
PRIMER_PAIR_33_PENALTY=1.669226
PRIMER_LEFT_33_PENALTY=0.513776
PRIMER_RIGHT_33_PENALTY=1.155450
PRIMER_LEFT_33_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_33_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_33=257,20
PRIMER_RIGHT_33=1187,21
PRIMER_LEFT_33_TM=59.486
PRIMER_RIGHT_33_TM=59.845
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=1.703379
PRIMER_LEFT_34_PENALTY=1.446550
PRIMER_RIGHT_34_PENALTY=0.256829
PRIMER_LEFT_34_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_34_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_34=729,21
PRIMER_RIGHT_34=1582,20
PRIMER_LEFT_34_TM=60.447
PRIMER_RIGHT_34_TM=60.257
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=854
PRIMER_PAIR_35_PENALTY=1.713170
PRIMER_LEFT_35_PENALTY=1.446550
PRIMER_RIGHT_35_PENALTY=0.266620
PRIMER_LEFT_35_SEQUENCE=CTGCCTGCACGAACTACCTAA
PRIMER_RIGHT_35_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_35=729,21
PRIMER_RIGHT_35=1500,20
PRIMER_LEFT_35_TM=60.447
PRIMER_RIGHT_35_TM=59.733
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.5000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=772
PRIMER_PAIR_36_PENALTY=1.740964
PRIMER_LEFT_36_PENALTY=1.484135
PRIMER_RIGHT_36_PENALTY=0.256829
PRIMER_LEFT_36_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_36_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_36=725,21
PRIMER_RIGHT_36=1582,20
PRIMER_LEFT_36_TM=59.516
PRIMER_RIGHT_36_TM=60.257
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.1000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=858
PRIMER_PAIR_37_PENALTY=1.750755
PRIMER_LEFT_37_PENALTY=1.484135
PRIMER_RIGHT_37_PENALTY=0.266620
PRIMER_LEFT_37_SEQUENCE=AGATCTGCCTGCACGAACTAC
PRIMER_RIGHT_37_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_37=725,21
PRIMER_RIGHT_37=1500,20
PRIMER_LEFT_37_TM=59.516
PRIMER_RIGHT_37_TM=59.733
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.1000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=776
PRIMER_PAIR_38_PENALTY=1.785200
PRIMER_LEFT_38_PENALTY=1.528370
PRIMER_RIGHT_38_PENALTY=0.256829
PRIMER_LEFT_38_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_38_SEQUENCE=GCGTGCCACATTTTCTTCTT
PRIMER_LEFT_38=726,20
PRIMER_RIGHT_38=1582,20
PRIMER_LEFT_38_TM=58.472
PRIMER_RIGHT_38_TM=60.257
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.1000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=857
PRIMER_PAIR_39_PENALTY=1.794991
PRIMER_LEFT_39_PENALTY=1.528370
PRIMER_RIGHT_39_PENALTY=0.266620
PRIMER_LEFT_39_SEQUENCE=GATCTGCCTGCACGAACTAC
PRIMER_RIGHT_39_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_39=726,20
PRIMER_RIGHT_39=1500,20
PRIMER_LEFT_39_TM=58.472
PRIMER_RIGHT_39_TM=59.733
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.1000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=775
PRIMER_PAIR_40_PENALTY=1.811843
PRIMER_LEFT_40_PENALTY=0.656393
PRIMER_RIGHT_40_PENALTY=1.155450
PRIMER_LEFT_40_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_40_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_40=379,20
PRIMER_RIGHT_40=1186,21
PRIMER_LEFT_40_TM=60.656
PRIMER_RIGHT_40_TM=59.845
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=808
PRIMER_PAIR_41_PENALTY=1.811843
PRIMER_LEFT_41_PENALTY=0.656393
PRIMER_RIGHT_41_PENALTY=1.155450
PRIMER_LEFT_41_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_41_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_41=379,20
PRIMER_RIGHT_41=1187,21
PRIMER_LEFT_41_TM=60.656
PRIMER_RIGHT_41_TM=59.845
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=809
PRIMER_PAIR_42_PENALTY=1.838560
PRIMER_LEFT_42_PENALTY=0.285121
PRIMER_RIGHT_42_PENALTY=1.553439
PRIMER_LEFT_42_SEQUENCE=ATCTGCCTGCACGAACTACC
PRIMER_RIGHT_42_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_42=727,20
PRIMER_RIGHT_42=1495,21
PRIMER_LEFT_42_TM=60.285
PRIMER_RIGHT_42_TM=59.447
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=769
PRIMER_PAIR_43_PENALTY=1.855887
PRIMER_LEFT_43_PENALTY=0.712754
PRIMER_RIGHT_43_PENALTY=1.143133
PRIMER_LEFT_43_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_43_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_43=531,20
PRIMER_RIGHT_43=1496,20
PRIMER_LEFT_43_TM=60.713
PRIMER_RIGHT_43_TM=61.143
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=1.855887
PRIMER_LEFT_44_PENALTY=0.712754
PRIMER_RIGHT_44_PENALTY=1.143133
PRIMER_LEFT_44_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_44_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_44=531,20
PRIMER_RIGHT_44=1497,20
PRIMER_LEFT_44_TM=60.713
PRIMER_RIGHT_44_TM=61.143
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=967
PRIMER_PAIR_45_PENALTY=1.855887
PRIMER_LEFT_45_PENALTY=0.712754
PRIMER_RIGHT_45_PENALTY=1.143133
PRIMER_LEFT_45_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_45_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_45=530,20
PRIMER_RIGHT_45=1496,20
PRIMER_LEFT_45_TM=60.713
PRIMER_RIGHT_45_TM=61.143
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=967
PRIMER_PAIR_46_PENALTY=1.855887
PRIMER_LEFT_46_PENALTY=0.712754
PRIMER_RIGHT_46_PENALTY=1.143133
PRIMER_LEFT_46_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_46_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_46=530,20
PRIMER_RIGHT_46=1497,20
PRIMER_LEFT_46_TM=60.713
PRIMER_RIGHT_46_TM=61.143
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=968
PRIMER_PAIR_47_PENALTY=1.868204
PRIMER_LEFT_47_PENALTY=0.712754
PRIMER_RIGHT_47_PENALTY=1.155450
PRIMER_LEFT_47_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_47_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_47=531,20
PRIMER_RIGHT_47=1186,21
PRIMER_LEFT_47_TM=60.713
PRIMER_RIGHT_47_TM=59.845
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=656
PRIMER_PAIR_48_PENALTY=1.868204
PRIMER_LEFT_48_PENALTY=0.712754
PRIMER_RIGHT_48_PENALTY=1.155450
PRIMER_LEFT_48_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_48_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_48=531,20
PRIMER_RIGHT_48=1187,21
PRIMER_LEFT_48_TM=60.713
PRIMER_RIGHT_48_TM=59.845
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=657
PRIMER_PAIR_49_PENALTY=1.868204
PRIMER_LEFT_49_PENALTY=0.712754
PRIMER_RIGHT_49_PENALTY=1.155450
PRIMER_LEFT_49_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_49_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_49=530,20
PRIMER_RIGHT_49=1186,21
PRIMER_LEFT_49_TM=60.713
PRIMER_RIGHT_49_TM=59.845
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=657
=
SEQUENCE_ID=MAGIC.433_4_11602000_11602078_ler-0_can-0_217630_11138_TRUE
SEQUENCE_TARGET=950,178
SEQUENCE_TEMPLATE=TAAGGATCAGGTAACAACATTNATTCCANAAAACTCAATTTTCAAGNTCAATGCATCAAATCTCTNNAACACCAATCACCAAGTAAACTTGAAACCNANAAAGCATTCTCTTTTATANATTTATNATCATNAATTNCCAAATCAANACATGCNANAACACTANCTGAAGCAGAACANTNAAAACCAGANCAAAANNGAGNCACGNNANCACTTACTCAGTGCCTTTCTGTCTGAGAATCCTAAATTGTTCAGGAGAAAGAATCGNACNCCACTCTTCTTCCGGCTTATTGACCGATTCAGGAGCAGAAGAACCCATTGNCACAATACGACCACCGTGNAAACCTCGACGGAGCGACNGAAATGACAAAGGAGATGATGAGAAACGAAGGAGGTTACGANGAGACNGAGAGGCNAAANNAGGTTTAACGAAAGCNGCTTTAATGGTGGAAACGAANGTGANAGACGTGGCTGAATAAACACGACCAGGTGTTATGATATTGAACGCCATTTGTTTGGCTTTTTATCTNCTCTCTTCCTTCTTCTCTTGACAACAGCTTTTTNTGACTCTCTCTCAATCNTTTCTCAGATTTTTCNTTTTCTCTGGAACACTCNTCTAANTTGGGCCTTACNTTTNGGNCNTTTANCCCAATATACATNCACTCGAAATTGCATTANTCTCTGTTCAAGTTTAGCTATGNCANCCTTGTCGGAATCATTCGTGTACCTGGTTCAAACCCTANNNNTGGTTTNATGAGGCNATGTGATGAGNCTCTGACAAAAGANAANTTNGAAGTTAAAGTCACATGGAAAAAANNAANCAACTAACGGCTGGTGAGAGAAGAAGAGTCGTTCGTATTTAAAACATGAGCATGAATATTTTGACCTCAATAAAGGGACTTTGNNTCANTAACATTCATATTCTTNTTTTTCCAACCTAACGCAATTNTGNAGACCGATCAATCAGACGGTTGTTTCTATACCACACAAGGNAANANNNNTANANTTTGGATCGANATTGGAACCAAAACTAGTAAGTGGGTTTNNTTATGCTTNGATTTNTTTTNNTTTNTGTTNTTTGNNNNTNGANNNNNTCGNTTCNATNNTTTGTTTNTNNNGANNNANNAANNNNCTNNNNCNNNNNNNANNNNNNNNANNNTNTNTCNAAANTTTNCNTNTTTTTNNTNCNTGGAATNTTTGTAGTGTACCCGAAAGTGAAAATTGACNATGANNCTGGTANATACAAGCAGANGGGTTCTTGTCTGAACTCNAACTATCTCCAAACTCGTGTCTCTGGTAATTTCTCTCTNCTTCNNTTTTTNTTTTNCTTTGAGTTTGACTCTCATTTAGATTTTGAGCTAGAANTTTAGGGTTGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTTTAGCTATATGTTTAGGAATTAGGAACATTGACTTGGTTTTTGANAGAGATCNTNNNNNNNNNNNNNATATGGGTTCTTTGTTGTTGCCAATTNAANCGAATGCAAANTTTNAANAGAATCTNTTCTGATGTTCTCTCTTTCTCGTTNGGACAGANAATAANCAAGAAGATGATTTACAAATATACGTGGCTAAGATTCCTAAGATTTACATACCAAGTGTCCTCATNTCTCANAGTGAATCCAAAGAAATGAATAAGCCTATNAGAGGAGCTGACATTGAAGTAAAACCAANAACCAAAGCCAGTCCAANCCTACGTCCACGCGCTNTTGTATCAAGTCCCGGTATGGNNNNTTNTTAGCTGNTTTNCATCTCNAANGTTTTCATTTNCTGGACTGTTTCCATGTGNTTTAGCTATAGTGTCACACAATGTTGAACTCAAATATNTCNAAAACAATGACTTNTGCTTTTGAATTTTCTCTTCTTTTAGATAATGATGCNATGATCGGCAACATGAACATGATTGAAGAAAGGAAAGCTAAGAAGGGTTTGAAGAGTAANGATCATATTAAGAGTAGGGCTTCTCAGCTTAAGAATGTTNATACCAATGTGAAGATTTCTCATCGTATAGTCGCTAAGCTACCGGGAGTCAATTCAAGGNATCACA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.914165
PRIMER_LEFT_0_PENALTY=0.010545
PRIMER_RIGHT_0_PENALTY=0.903619
PRIMER_LEFT_0_SEQUENCE=AACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_0_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_0=823,20
PRIMER_RIGHT_0=1502,20
PRIMER_LEFT_0_TM=59.989
PRIMER_RIGHT_0_TM=60.904
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=680
PRIMER_PAIR_1_PENALTY=1.305661
PRIMER_LEFT_1_PENALTY=0.402042
PRIMER_RIGHT_1_PENALTY=0.903619
PRIMER_LEFT_1_SEQUENCE=ACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_1_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_1=824,20
PRIMER_RIGHT_1=1502,20
PRIMER_LEFT_1_TM=59.598
PRIMER_RIGHT_1_TM=60.904
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=679
PRIMER_PAIR_2_PENALTY=1.438951
PRIMER_LEFT_2_PENALTY=0.010545
PRIMER_RIGHT_2_PENALTY=1.428405
PRIMER_LEFT_2_SEQUENCE=AACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_2_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_2=823,20
PRIMER_RIGHT_2=1293,21
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=60.428
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=471
PRIMER_PAIR_3_PENALTY=1.571684
PRIMER_LEFT_3_PENALTY=0.668065
PRIMER_RIGHT_3_PENALTY=0.903619
PRIMER_LEFT_3_SEQUENCE=CGGCTGGTGAGAGAAGAAGA
PRIMER_RIGHT_3_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_3=825,20
PRIMER_RIGHT_3=1502,20
PRIMER_LEFT_3_TM=60.668
PRIMER_RIGHT_3_TM=60.904
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=678
PRIMER_PAIR_4_PENALTY=1.650084
PRIMER_LEFT_4_PENALTY=0.010545
PRIMER_RIGHT_4_PENALTY=1.639539
PRIMER_LEFT_4_SEQUENCE=AACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_4_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_4=823,20
PRIMER_RIGHT_4=1294,21
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=59.360
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=472
PRIMER_PAIR_5_PENALTY=1.830447
PRIMER_LEFT_5_PENALTY=0.402042
PRIMER_RIGHT_5_PENALTY=1.428405
PRIMER_LEFT_5_SEQUENCE=ACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_5_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_5=824,20
PRIMER_RIGHT_5=1293,21
PRIMER_LEFT_5_TM=59.598
PRIMER_RIGHT_5_TM=60.428
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=52.381
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=470
PRIMER_PAIR_6_PENALTY=1.904300
PRIMER_LEFT_6_PENALTY=1.000680
PRIMER_RIGHT_6_PENALTY=0.903619
PRIMER_LEFT_6_SEQUENCE=TAACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_6_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_6=822,21
PRIMER_RIGHT_6=1502,20
PRIMER_LEFT_6_TM=60.001
PRIMER_RIGHT_6_TM=60.904
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=681
PRIMER_PAIR_7_PENALTY=2.041581
PRIMER_LEFT_7_PENALTY=0.402042
PRIMER_RIGHT_7_PENALTY=1.639539
PRIMER_LEFT_7_SEQUENCE=ACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_7_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_7=824,20
PRIMER_RIGHT_7=1294,21
PRIMER_LEFT_7_TM=59.598
PRIMER_RIGHT_7_TM=59.360
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=471
PRIMER_PAIR_8_PENALTY=2.096470
PRIMER_LEFT_8_PENALTY=0.668065
PRIMER_RIGHT_8_PENALTY=1.428405
PRIMER_LEFT_8_SEQUENCE=CGGCTGGTGAGAGAAGAAGA
PRIMER_RIGHT_8_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_8=825,20
PRIMER_RIGHT_8=1293,21
PRIMER_LEFT_8_TM=60.668
PRIMER_RIGHT_8_TM=60.428
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=469
PRIMER_PAIR_9_PENALTY=2.131841
PRIMER_LEFT_9_PENALTY=0.010545
PRIMER_RIGHT_9_PENALTY=2.121295
PRIMER_LEFT_9_SEQUENCE=AACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_9_SEQUENCE=ATTGGCAACAACAAAGAACCC
PRIMER_LEFT_9=823,20
PRIMER_RIGHT_9=1503,21
PRIMER_LEFT_9_TM=59.989
PRIMER_RIGHT_9_TM=61.121
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=681
PRIMER_PAIR_10_PENALTY=2.144531
PRIMER_LEFT_10_PENALTY=0.716126
PRIMER_RIGHT_10_PENALTY=1.428405
PRIMER_LEFT_10_SEQUENCE=CAGGAGCAGAAGAACCCATT
PRIMER_RIGHT_10_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_10=297,20
PRIMER_RIGHT_10=1293,21
PRIMER_LEFT_10_TM=59.284
PRIMER_RIGHT_10_TM=60.428
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=997
PRIMER_PAIR_11_PENALTY=2.160353
PRIMER_LEFT_11_PENALTY=0.731948
PRIMER_RIGHT_11_PENALTY=1.428405
PRIMER_LEFT_11_SEQUENCE=ATTGAACGCCATTTGTTTGG
PRIMER_RIGHT_11_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_11=496,20
PRIMER_RIGHT_11=1293,21
PRIMER_LEFT_11_TM=60.732
PRIMER_RIGHT_11_TM=60.428
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.8000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=798
PRIMER_PAIR_12_PENALTY=2.229494
PRIMER_LEFT_12_PENALTY=0.801088
PRIMER_RIGHT_12_PENALTY=1.428405
PRIMER_LEFT_12_SEQUENCE=ACGTGGCTGAATAAACACGA
PRIMER_RIGHT_12_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_12=462,20
PRIMER_RIGHT_12=1293,21
PRIMER_LEFT_12_TM=59.199
PRIMER_RIGHT_12_TM=60.428
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=832
PRIMER_PAIR_13_PENALTY=2.279247
PRIMER_LEFT_13_PENALTY=1.375627
PRIMER_RIGHT_13_PENALTY=0.903619
PRIMER_LEFT_13_SEQUENCE=CTAACGGCTGGTGAGAGAAGA
PRIMER_RIGHT_13_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_13=821,21
PRIMER_RIGHT_13=1502,20
PRIMER_LEFT_13_TM=59.624
PRIMER_RIGHT_13_TM=60.904
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=682
PRIMER_PAIR_14_PENALTY=2.307604
PRIMER_LEFT_14_PENALTY=0.668065
PRIMER_RIGHT_14_PENALTY=1.639539
PRIMER_LEFT_14_SEQUENCE=CGGCTGGTGAGAGAAGAAGA
PRIMER_RIGHT_14_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_14=825,20
PRIMER_RIGHT_14=1294,21
PRIMER_LEFT_14_TM=60.668
PRIMER_RIGHT_14_TM=59.360
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=470
PRIMER_PAIR_15_PENALTY=2.307809
PRIMER_LEFT_15_PENALTY=1.404189
PRIMER_RIGHT_15_PENALTY=0.903619
PRIMER_LEFT_15_SEQUENCE=TAACGGCTGGTGAGAGAAGA
PRIMER_RIGHT_15_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_15=822,20
PRIMER_RIGHT_15=1502,20
PRIMER_LEFT_15_TM=58.596
PRIMER_RIGHT_15_TM=60.904
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=681
PRIMER_PAIR_16_PENALTY=2.341106
PRIMER_LEFT_16_PENALTY=0.912701
PRIMER_RIGHT_16_PENALTY=1.428405
PRIMER_LEFT_16_SEQUENCE=TTCAGGAGCAGAAGAACCCA
PRIMER_RIGHT_16_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_16=295,20
PRIMER_RIGHT_16=1293,21
PRIMER_LEFT_16_TM=60.913
PRIMER_RIGHT_16_TM=60.428
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=999
PRIMER_PAIR_17_PENALTY=2.341444
PRIMER_LEFT_17_PENALTY=1.437825
PRIMER_RIGHT_17_PENALTY=0.903619
PRIMER_LEFT_17_SEQUENCE=CAACTAACGGCTGGTGAGAGA
PRIMER_RIGHT_17_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_17=818,21
PRIMER_RIGHT_17=1502,20
PRIMER_LEFT_17_TM=60.438
PRIMER_RIGHT_17_TM=60.904
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=685
PRIMER_PAIR_18_PENALTY=2.355665
PRIMER_LEFT_18_PENALTY=0.716126
PRIMER_RIGHT_18_PENALTY=1.639539
PRIMER_LEFT_18_SEQUENCE=CAGGAGCAGAAGAACCCATT
PRIMER_RIGHT_18_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_18=297,20
PRIMER_RIGHT_18=1294,21
PRIMER_LEFT_18_TM=59.284
PRIMER_RIGHT_18_TM=59.360
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=998
PRIMER_PAIR_19_PENALTY=2.371486
PRIMER_LEFT_19_PENALTY=0.731948
PRIMER_RIGHT_19_PENALTY=1.639539
PRIMER_LEFT_19_SEQUENCE=ATTGAACGCCATTTGTTTGG
PRIMER_RIGHT_19_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_19=496,20
PRIMER_RIGHT_19=1294,21
PRIMER_LEFT_19_TM=60.732
PRIMER_RIGHT_19_TM=59.360
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.8000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=799
PRIMER_PAIR_20_PENALTY=2.380835
PRIMER_LEFT_20_PENALTY=1.477216
PRIMER_RIGHT_20_PENALTY=0.903619
PRIMER_LEFT_20_SEQUENCE=CAACTAACGGCTGGTGAGAG
PRIMER_RIGHT_20_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_20=818,20
PRIMER_RIGHT_20=1502,20
PRIMER_LEFT_20_TM=58.523
PRIMER_RIGHT_20_TM=60.904
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=685
PRIMER_PAIR_21_PENALTY=2.429086
PRIMER_LEFT_21_PENALTY=1.000680
PRIMER_RIGHT_21_PENALTY=1.428405
PRIMER_LEFT_21_SEQUENCE=TAACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_21_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_21=822,21
PRIMER_RIGHT_21=1293,21
PRIMER_LEFT_21_TM=60.001
PRIMER_RIGHT_21_TM=60.428
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=472
PRIMER_PAIR_22_PENALTY=2.435361
PRIMER_LEFT_22_PENALTY=0.010545
PRIMER_RIGHT_22_PENALTY=2.424816
PRIMER_LEFT_22_SEQUENCE=AACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_22_SEQUENCE=ATTGGCAACAACAAAGAACC
PRIMER_LEFT_22=823,20
PRIMER_RIGHT_22=1503,20
PRIMER_LEFT_22_TM=59.989
PRIMER_RIGHT_22_TM=57.575
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=681
PRIMER_PAIR_23_PENALTY=2.440627
PRIMER_LEFT_23_PENALTY=0.801088
PRIMER_RIGHT_23_PENALTY=1.639539
PRIMER_LEFT_23_SEQUENCE=ACGTGGCTGAATAAACACGA
PRIMER_RIGHT_23_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_23=462,20
PRIMER_RIGHT_23=1294,21
PRIMER_LEFT_23_TM=59.199
PRIMER_RIGHT_23_TM=59.360
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=833
PRIMER_PAIR_24_PENALTY=2.441242
PRIMER_LEFT_24_PENALTY=0.010545
PRIMER_RIGHT_24_PENALTY=2.430697
PRIMER_LEFT_24_SEQUENCE=AACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_24_SEQUENCE=TGGCAACAACAAAGAACCCA
PRIMER_LEFT_24=823,20
PRIMER_RIGHT_24=1501,20
PRIMER_LEFT_24_TM=59.989
PRIMER_RIGHT_24_TM=62.431
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=679
PRIMER_PAIR_25_PENALTY=2.523337
PRIMER_LEFT_25_PENALTY=0.402042
PRIMER_RIGHT_25_PENALTY=2.121295
PRIMER_LEFT_25_SEQUENCE=ACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_25_SEQUENCE=ATTGGCAACAACAAAGAACCC
PRIMER_LEFT_25=824,20
PRIMER_RIGHT_25=1503,21
PRIMER_LEFT_25_TM=59.598
PRIMER_RIGHT_25_TM=61.121
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=680
PRIMER_PAIR_26_PENALTY=2.552239
PRIMER_LEFT_26_PENALTY=0.912701
PRIMER_RIGHT_26_PENALTY=1.639539
PRIMER_LEFT_26_SEQUENCE=TTCAGGAGCAGAAGAACCCA
PRIMER_RIGHT_26_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_26=295,20
PRIMER_RIGHT_26=1294,21
PRIMER_LEFT_26_TM=60.913
PRIMER_RIGHT_26_TM=59.360
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=1000
PRIMER_PAIR_27_PENALTY=2.614505
PRIMER_LEFT_27_PENALTY=0.010545
PRIMER_RIGHT_27_PENALTY=2.603959
PRIMER_LEFT_27_SEQUENCE=AACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_27_SEQUENCE=TACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_27=823,20
PRIMER_RIGHT_27=1295,22
PRIMER_LEFT_27_TM=59.989
PRIMER_RIGHT_27_TM=59.396
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=473
PRIMER_PAIR_28_PENALTY=2.640219
PRIMER_LEFT_28_PENALTY=1.000680
PRIMER_RIGHT_28_PENALTY=1.639539
PRIMER_LEFT_28_SEQUENCE=TAACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_28_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_28=822,21
PRIMER_RIGHT_28=1294,21
PRIMER_LEFT_28_TM=60.001
PRIMER_RIGHT_28_TM=59.360
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=473
PRIMER_PAIR_29_PENALTY=2.789360
PRIMER_LEFT_29_PENALTY=0.668065
PRIMER_RIGHT_29_PENALTY=2.121295
PRIMER_LEFT_29_SEQUENCE=CGGCTGGTGAGAGAAGAAGA
PRIMER_RIGHT_29_SEQUENCE=ATTGGCAACAACAAAGAACCC
PRIMER_LEFT_29=825,20
PRIMER_RIGHT_29=1503,21
PRIMER_LEFT_29_TM=60.668
PRIMER_RIGHT_29_TM=61.121
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=9.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=679
PRIMER_PAIR_30_PENALTY=2.804033
PRIMER_LEFT_30_PENALTY=1.375627
PRIMER_RIGHT_30_PENALTY=1.428405
PRIMER_LEFT_30_SEQUENCE=CTAACGGCTGGTGAGAGAAGA
PRIMER_RIGHT_30_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_30=821,21
PRIMER_RIGHT_30=1293,21
PRIMER_LEFT_30_TM=59.624
PRIMER_RIGHT_30_TM=60.428
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=473
PRIMER_PAIR_31_PENALTY=2.826858
PRIMER_LEFT_31_PENALTY=0.402042
PRIMER_RIGHT_31_PENALTY=2.424816
PRIMER_LEFT_31_SEQUENCE=ACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_31_SEQUENCE=ATTGGCAACAACAAAGAACC
PRIMER_LEFT_31=824,20
PRIMER_RIGHT_31=1503,20
PRIMER_LEFT_31_TM=59.598
PRIMER_RIGHT_31_TM=57.575
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=680
PRIMER_PAIR_32_PENALTY=2.832595
PRIMER_LEFT_32_PENALTY=1.404189
PRIMER_RIGHT_32_PENALTY=1.428405
PRIMER_LEFT_32_SEQUENCE=TAACGGCTGGTGAGAGAAGA
PRIMER_RIGHT_32_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_32=822,20
PRIMER_RIGHT_32=1293,21
PRIMER_LEFT_32_TM=58.596
PRIMER_RIGHT_32_TM=60.428
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=472
PRIMER_PAIR_33_PENALTY=2.832739
PRIMER_LEFT_33_PENALTY=0.402042
PRIMER_RIGHT_33_PENALTY=2.430697
PRIMER_LEFT_33_SEQUENCE=ACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_33_SEQUENCE=TGGCAACAACAAAGAACCCA
PRIMER_LEFT_33=824,20
PRIMER_RIGHT_33=1501,20
PRIMER_LEFT_33_TM=59.598
PRIMER_RIGHT_33_TM=62.431
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=678
PRIMER_PAIR_34_PENALTY=2.840095
PRIMER_LEFT_34_PENALTY=1.936476
PRIMER_RIGHT_34_PENALTY=0.903619
PRIMER_LEFT_34_SEQUENCE=AACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_34_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_34=823,21
PRIMER_RIGHT_34=1502,20
PRIMER_LEFT_34_TM=60.936
PRIMER_RIGHT_34_TM=60.904
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=680
PRIMER_PAIR_35_PENALTY=2.866230
PRIMER_LEFT_35_PENALTY=1.437825
PRIMER_RIGHT_35_PENALTY=1.428405
PRIMER_LEFT_35_SEQUENCE=CAACTAACGGCTGGTGAGAGA
PRIMER_RIGHT_35_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_35=818,21
PRIMER_RIGHT_35=1293,21
PRIMER_LEFT_35_TM=60.438
PRIMER_RIGHT_35_TM=60.428
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=476
PRIMER_PAIR_36_PENALTY=2.905621
PRIMER_LEFT_36_PENALTY=1.477216
PRIMER_RIGHT_36_PENALTY=1.428405
PRIMER_LEFT_36_SEQUENCE=CAACTAACGGCTGGTGAGAG
PRIMER_RIGHT_36_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_36=818,20
PRIMER_RIGHT_36=1293,21
PRIMER_LEFT_36_TM=58.523
PRIMER_RIGHT_36_TM=60.428
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=476
PRIMER_PAIR_37_PENALTY=2.909868
PRIMER_LEFT_37_PENALTY=2.006249
PRIMER_RIGHT_37_PENALTY=0.903619
PRIMER_LEFT_37_SEQUENCE=AACTAACGGCTGGTGAGAGAA
PRIMER_RIGHT_37_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_37=819,21
PRIMER_RIGHT_37=1502,20
PRIMER_LEFT_37_TM=58.994
PRIMER_RIGHT_37_TM=60.904
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=684
PRIMER_PAIR_38_PENALTY=2.960470
PRIMER_LEFT_38_PENALTY=2.056850
PRIMER_RIGHT_38_PENALTY=0.903619
PRIMER_LEFT_38_SEQUENCE=AACTAACGGCTGGTGAGAGAAG
PRIMER_RIGHT_38_SEQUENCE=TTGGCAACAACAAAGAACCC
PRIMER_LEFT_38=819,22
PRIMER_RIGHT_38=1502,20
PRIMER_LEFT_38_TM=59.943
PRIMER_RIGHT_38_TM=60.904
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=684
PRIMER_PAIR_39_PENALTY=3.006001
PRIMER_LEFT_39_PENALTY=0.402042
PRIMER_RIGHT_39_PENALTY=2.603959
PRIMER_LEFT_39_SEQUENCE=ACGGCTGGTGAGAGAAGAAG
PRIMER_RIGHT_39_SEQUENCE=TACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_39=824,20
PRIMER_RIGHT_39=1295,22
PRIMER_LEFT_39_TM=59.598
PRIMER_RIGHT_39_TM=59.396
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=472
PRIMER_PAIR_40_PENALTY=3.015166
PRIMER_LEFT_40_PENALTY=1.375627
PRIMER_RIGHT_40_PENALTY=1.639539
PRIMER_LEFT_40_SEQUENCE=CTAACGGCTGGTGAGAGAAGA
PRIMER_RIGHT_40_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_40=821,21
PRIMER_RIGHT_40=1294,21
PRIMER_LEFT_40_TM=59.624
PRIMER_RIGHT_40_TM=59.360
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=474
PRIMER_PAIR_41_PENALTY=3.043728
PRIMER_LEFT_41_PENALTY=1.404189
PRIMER_RIGHT_41_PENALTY=1.639539
PRIMER_LEFT_41_SEQUENCE=TAACGGCTGGTGAGAGAAGA
PRIMER_RIGHT_41_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_41=822,20
PRIMER_RIGHT_41=1294,21
PRIMER_LEFT_41_TM=58.596
PRIMER_RIGHT_41_TM=59.360
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=473
PRIMER_PAIR_42_PENALTY=3.077363
PRIMER_LEFT_42_PENALTY=1.437825
PRIMER_RIGHT_42_PENALTY=1.639539
PRIMER_LEFT_42_SEQUENCE=CAACTAACGGCTGGTGAGAGA
PRIMER_RIGHT_42_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_42=818,21
PRIMER_RIGHT_42=1294,21
PRIMER_LEFT_42_TM=60.438
PRIMER_RIGHT_42_TM=59.360
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=477
PRIMER_PAIR_43_PENALTY=3.092881
PRIMER_LEFT_43_PENALTY=0.668065
PRIMER_RIGHT_43_PENALTY=2.424816
PRIMER_LEFT_43_SEQUENCE=CGGCTGGTGAGAGAAGAAGA
PRIMER_RIGHT_43_SEQUENCE=ATTGGCAACAACAAAGAACC
PRIMER_LEFT_43=825,20
PRIMER_RIGHT_43=1503,20
PRIMER_LEFT_43_TM=60.668
PRIMER_RIGHT_43_TM=57.575
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=679
PRIMER_PAIR_44_PENALTY=3.098762
PRIMER_LEFT_44_PENALTY=0.668065
PRIMER_RIGHT_44_PENALTY=2.430697
PRIMER_LEFT_44_SEQUENCE=CGGCTGGTGAGAGAAGAAGA
PRIMER_RIGHT_44_SEQUENCE=TGGCAACAACAAAGAACCCA
PRIMER_LEFT_44=825,20
PRIMER_RIGHT_44=1501,20
PRIMER_LEFT_44_TM=60.668
PRIMER_RIGHT_44_TM=62.431
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=677
PRIMER_PAIR_45_PENALTY=3.104407
PRIMER_LEFT_45_PENALTY=1.676001
PRIMER_RIGHT_45_PENALTY=1.428405
PRIMER_LEFT_45_SEQUENCE=ATTCAGGAGCAGAAGAACCC
PRIMER_RIGHT_45_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_45=294,20
PRIMER_RIGHT_45=1293,21
PRIMER_LEFT_45_TM=58.324
PRIMER_RIGHT_45_TM=60.428
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=1000
PRIMER_PAIR_46_PENALTY=3.116755
PRIMER_LEFT_46_PENALTY=1.477216
PRIMER_RIGHT_46_PENALTY=1.639539
PRIMER_LEFT_46_SEQUENCE=CAACTAACGGCTGGTGAGAG
PRIMER_RIGHT_46_SEQUENCE=ACCAGAGACACGAGTTTGGAG
PRIMER_LEFT_46=818,20
PRIMER_RIGHT_46=1294,21
PRIMER_LEFT_46_TM=58.523
PRIMER_RIGHT_46_TM=59.360
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=477
PRIMER_PAIR_47_PENALTY=3.121976
PRIMER_LEFT_47_PENALTY=1.000680
PRIMER_RIGHT_47_PENALTY=2.121295
PRIMER_LEFT_47_SEQUENCE=TAACGGCTGGTGAGAGAAGAA
PRIMER_RIGHT_47_SEQUENCE=ATTGGCAACAACAAAGAACCC
PRIMER_LEFT_47=822,21
PRIMER_RIGHT_47=1503,21
PRIMER_LEFT_47_TM=60.001
PRIMER_RIGHT_47_TM=61.121
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=682
PRIMER_PAIR_48_PENALTY=3.152833
PRIMER_LEFT_48_PENALTY=1.724428
PRIMER_RIGHT_48_PENALTY=1.428405
PRIMER_LEFT_48_SEQUENCE=ACGCCATTTGTTTGGCTTTT
PRIMER_RIGHT_48_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_48=501,20
PRIMER_RIGHT_48=1293,21
PRIMER_LEFT_48_TM=61.724
PRIMER_RIGHT_48_TM=60.428
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=793
PRIMER_PAIR_49_PENALTY=3.200002
PRIMER_LEFT_49_PENALTY=1.771597
PRIMER_RIGHT_49_PENALTY=1.428405
PRIMER_LEFT_49_SEQUENCE=GGCTGAATAAACACGACCAG
PRIMER_RIGHT_49_SEQUENCE=CCAGAGACACGAGTTTGGAGA
PRIMER_LEFT_49=466,20
PRIMER_RIGHT_49=1293,21
PRIMER_LEFT_49_TM=58.228
PRIMER_RIGHT_49_TM=60.428
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=828
=
SEQUENCE_ID=MAGIC.433_2_5766113_5766391_ct-1_kn-0_27924_116104_TRUE
SEQUENCE_TARGET=950,378
SEQUENCE_TEMPLATE=NNNNNNGATGTGAATATGTGTTGATATTACCATGGCAGTTCTTTAAATCCAACAGATCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTCCTTGTCTTGTCCAACTCCTAAATNANNTCTGTCAAGAANNANTNNNNNNNNNNTNTTTNNNNNAAAANANAGANGNGTTNTCTAATATTATATAGTAACAAGTTTAGAGTTTTCGTGTTCGTTGAGGANTTTTCGTTTCCACATTGTCAAATACTATAATAAGTTAGAATTGTTNTTTTTTATTTCTTCTTCTTTTTATTGATTTTCTTGACAAATGGGTGGATGACAATAAGGAGTGTTGAGTTTNTCTATNTGGTAGTCGTAAGCCAATAAAAACTATATAACAGTCTNTATCACTTGAATTCTGACTGATTAAATTCTTTTAAAACGAATTATTCTCTTTGGNAATNTTTCTCNAAAACTTATTTTAATATTATNCCTTGACTATTTGATTATTCTTCNCTTTTATTATTGACTTTTCTCCTGAGAAGAACAAAACCTTGTCTGATTCGGTTTGTCAAGGTGTANNNNNNNNNNNTNTTTTTTTATCANTTCTCATATATANNNNNNNGTTGANATGTATTAGTCAAGAATTTTCTATACTTTTTAAGCAAAATCATGAAGGTTTCTATGAGGCTTCTNAGTCATTTCTTGAGCATGTTCATANNTGANGTNCCTGTAGCCCTCATAGATTAAGACCACAAAACTTGGTCTACACAAGTTTTAACAAAANAAATAGCATNAAAANCTTTGTCNGGTAAACTTANATACTTTGAATTCTGNCATAACTANNNNNTATGGTATAAGTTATGAGTGGTNAATAAGGTTCCTCATAANTTGGTTAANGAACATTTCTTAATTAG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_8403075_8403209_oy-0_edi-0_81751_52465_TRUE
SEQUENCE_TARGET=950,234
SEQUENCE_TEMPLATE=TTTGTTCTAGCACCCAACGCCTGCAAATCAAAAGAACCCTATTTCCATTAATACTTTAGAGAAAGACCTCTCAGTGTTTAACAAAAANATTACAGCTCCCTAAAAACAACACCATTCAGATAACAGAAGAAGATGAACGAGAAATTTCGTGAAANTCTGCAACAGATCAAATTGTATTCTTCTTTTAGGAATAGAAACAAACNGATAATGTGCAAACAAAAACGGTACCTTTCTTTACGTTGTTGAATGTGTCTCATGCGTCTATTGTAATGGTTTTCAATATACTTCTTCGCTGCAGCAACTTTCTCCAATGTTGAATTTGAAACCAACCCTTCATCCTCAAAATTATCCTCAACCTCCGACGTCACCTTCGCCAGCACCACCTCATCTTCCTCTTGATTCTCCANTTTTGGTTCTCGNAAAAAAAAAAAACTCTTTTGCTTCTCTCTAAATTCAGAGAATCTCTCGCATTTCTCTTTCCTTGGTCAATTGTGAGAACAATATGATTGCGAGAACGNNNNNAAGTCTCTCTTCATCTCCATCGATCAATGGCAATTTACAAGTTTGGATTTTTGAAATNTAGAAAAAGGAATCACGAATNNTNACNTNGANATTTTTGGTTTTANTANTTTTGCTATCNTCNGGNAAAAGGAAGATTAAACCACAAATCAAAACCNNNNNNNNGGATTGAAGGAAAATTTTGACAAACCGGAAACNACAGTAAAACCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAANGGNCACAACTTGTAGTACACAAAATGTNATAGTAGACTTCATTTGCNGACATGCATCACCAGTGTTCNAAAACTGTCAAGTTGANGCCTGAAACGTGACATAGNAATGTTATAATTGNTCTGTCAATNNAANNANAGAGCAATGTTATAACTGCTCAACAACGTGATAAGTCNGGCGAAAGTTTNNGATTTTCAATTCAAAAATGNGAAAATATACTANATATGTACTCATATACTAAATATACTAAAAAGTNTTTTTGTGAATTTATTTGAGTTTANGTATGTTAACGTGTATGGCCGTTTGATATGGGAAGTGGATGTNTATTTTTNGCCAAGGGAGATNGTAGTGTTGCTAATATTGTTTTTTTGAACGACTTGATCCCTCATTGAAGGTTTAGTTCTATATTAAACTTGTCTGTAATTTGAAGAAGTCATCGAGNAAAGCCTTGGGTGGCTTTGTTTGTATGTATCTTTGTTTGGTTTGGTTAAATGANTTTTAAAGGTAATATGTTGAATTGATCAATGGATGTGAAAGGATGATCAAGAAACTATGTTCATGAAAATTTTGAAATTTGTGTTCAACTTTTATGCAAAAGATGAGCAGAATTACGATCC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14472179_14472372_sf-2_ws-0_2891_17484_TRUE
SEQUENCE_TARGET=950,293
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCATNGGTGTTGTNTAGATGAGAACCTATCAACACANNAGGATNAAGNACTTGGTATATTATAATAAAATTATNTGTGNTTTNNNNGAAGAGAATTATGGTCNTTTTACGAATCTTTGTAATGTTTGGTATCTACGATGGTGCATACCGCGAGTTAGCCAAATGTTATGTATTTANTNGGAAACGCGTTTCCATGACGTTTTTTGTGTTTCCAACCTCAGAGCGAATTTCCNTGCNNCCNTNNCNATCGTCNTTGGNATCTGCTCCACCGNCNATTNANATCCCATGCATTCTTGNTGGGAATCANTGTCCAAGCAATNNNNNNNNNNNNTNTCTTGCATCATTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTNTNNTTNTATNTTATTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_10479262_10479366_ct-1_tsu-0_853901_2047_TRUE
SEQUENCE_TARGET=950,204
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_18174547_18174977_no-0_po-0_9490_4739_TRUE
SEQUENCE_TARGET=950,530
SEQUENCE_TEMPLATE=ACTTTTANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANGTTGNCTTNTGGAATCCNNNTTNNNNNNNNNNNNNNNNNNNNNTGANAGGGTCCTGGAGGAGTTTGGTGTGATGTAGATGTTGTTGAGTTTCAATATTATGGTGCACCTGCGCAAACNCCTAAAGAGCAGGTGTATACAGAGCTTGTTGATGATCTGAGAGGCAGTGGTTCGTCCATNGGATCTGGTTCACAGGTAANTTNTGTACTAAACTTGCANATANGCTTGTATTATCGANNNNNNNNNNNNNNNCATCTGGCCATGCCTCTCNTCTTNTAGGTGGCTAGNCAAGAGACATATGGAACCTTAGGTGCAATTGTAAAGAGTAAAACTGGTATNAGACAGGTTGGNTTCCTAACAAACCGGCATGTTGCAGTTGATTTAGACTACCCAAGCCAGAAGATGTTTCATCCATTGCCACCTAGCCTCGGACCCGGAGTCTACTTAGGTGCAGTGGAAAGAGCAACTTCATTCATTACCGATGACCTTTGGTATGGCATTTTCGCTGGAACTAACCCAGGTAGCCNAGTAANAATNTATANNNNTAACTCTNATGTATAATGGNGTAGCACNANTTTAATTATCGGTTTCTCTNTTTCTGCAGAAACATTTGTTCGAGCTGATGGAGCNTTCATTCCATTTGCNGAGGACTTCAATANGAACNATGTAACCACAACNGTCAAAGGGNTTGGTGAGATTGGTGATATCCATGCTACTGATCTACAGTCTCCAGTTAACAGTCTAATAGGAAGAAAAGTTGTCAAAGTTGGAAGAAGCTCGGGTTTGACCACTGGGACCATAATGGCTTACGCGTTGGAATACAATGACGAGAAAGGNATTTGTTTCNTAACNGATTTTCTTGTTGTTGGTGAAAACCAGCAGACTTTTGATCTTGAGGGTGATAGTGGAAGCCTTATTCTGTTGGCTGCGNGCNATGAGAAGAANGAGAAACCGCGACCTGTTGGAATNATNTGGGGAGGAACAGCAAACAGGGGACGGTTGAAGCTAAAAGTGGGGGAACAACCTGAGAATTGGACAAGTGGAGTTGATCTTGGCAGAGTTCTTAATCTTCTTGAGCTTGATCTCATTACTTCCAATGAGGGTCTTCAAGGTCTGTGTNATTNCTTAGAGTCTTTTTGCAGTACTTTTAGTATACACTGTTTCCTACAAAGTCTCTTTTGCTGAGTGCAGCTGCGGTGCTGGAACAGAGAAACGGTATTATNTGTGCTGCGGTTGATTCCACCGTCGTGGAATCATCTCCTGGTGTGTGCAATATTTCCAGGTGTAAAACGGGTGANAACTTTGAGCCTATTAACTTGAATGTCCAGCAAGTTCTTATAGAAGACGACAACTCAAACATTCANCCAGAGTTCCAAATAGAAGANGTGTTAGAATCAGTGGCTGTGNTCGAAGAACACCAGTTCATTCCAAGTTCAAGCAACAACGGATCCNCACTNCATCAGAAACNTAATGGACCAGAGAATCTNGAATCCAAGAACCTGTCTTNGCTTAAGACTAGTAGCTCAGGTGATGAGANNGGCTTTTCTTTGCAGTTAGGTGAGTCAGATACAAAGAAAAGAAAGCGAACNGATTCACCNNATGGTTCTCAAGAANATGAAGAGTGAGGGNNATGCTCATGAGACTTCATAANATACCAGAGGAGTCAATAGTAACATCACTATTATTTTTCCACTGCNTTTTTTCTCCAGAGGAAAGATATTCAGAATAGCATATTTGTANTGGCTAATGCCAAGGGATAGTCAAACGTTATGAGCATATATGTGTTGGTGATTTTATGAACACCTATAATTTTGTTGTNCCTTANGAACATCTNGGAGCATAAGCAATTGCAATGAAACNTCCCAGAAGGAATNGTTATAAGTGTAAGTAGATACANCCCTATGGATTAATTGGANTACAAAAACGCTCACAAGACNNNGANNGCCTAGTNGCTAGTCNGTAGATACAGNTGGCCAGCCTTGGTCAGCTTGAAACTTTACAAATTCAGCGAGAAGCTTGATTTCTTNGTCCTGCAACCGNGGTCCAAAGGTACATTGTCCTCTCGGTGTGCATTTCTCACCNAATCCCTGANGTAAATCATCAGACATACTTCAGATCATCNAGCTAAGTGGAAGACAATTCTCAGTTTTATACAAGGAATTTCTTCAAGGAGATCTCACCGGCATNCTTCCNTTTCCAAAGTAGGTAACACGATATATTTCTTCCTCAGTATCAACTCCATTTCTGTAGAAAAGTAAATAAACCATTGCAAATCTTAAGAGATAANGATCTGTGTAACCGATTTTTCCTGTCAGAATATTATAGATGCACACAGCACACAGTTCTTTGAGTGTCCAGCATAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.132901
PRIMER_LEFT_0_PENALTY=0.013872
PRIMER_RIGHT_0_PENALTY=0.119028
PRIMER_LEFT_0_SEQUENCE=CAAAGTTGGAAGAAGCTCGG
PRIMER_RIGHT_0_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_0=828,20
PRIMER_RIGHT_0=1512,20
PRIMER_LEFT_0_TM=59.986
PRIMER_RIGHT_0_TM=59.881
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=685
PRIMER_PAIR_1_PENALTY=0.365754
PRIMER_LEFT_1_PENALTY=0.246726
PRIMER_RIGHT_1_PENALTY=0.119028
PRIMER_LEFT_1_SEQUENCE=AAGTTGGAAGAAGCTCGGGT
PRIMER_RIGHT_1_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_1=830,20
PRIMER_RIGHT_1=1512,20
PRIMER_LEFT_1_TM=60.247
PRIMER_RIGHT_1_TM=59.881
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=683
PRIMER_PAIR_2_PENALTY=0.365754
PRIMER_LEFT_2_PENALTY=0.246726
PRIMER_RIGHT_2_PENALTY=0.119028
PRIMER_LEFT_2_SEQUENCE=AAGTTGGAAGAAGCTCGGGT
PRIMER_RIGHT_2_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_2=830,20
PRIMER_RIGHT_2=1514,20
PRIMER_LEFT_2_TM=60.247
PRIMER_RIGHT_2_TM=59.881
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=685
PRIMER_PAIR_3_PENALTY=0.397013
PRIMER_LEFT_3_PENALTY=0.277985
PRIMER_RIGHT_3_PENALTY=0.119028
PRIMER_LEFT_3_SEQUENCE=TGTTGGTGAAAACCAGCAGA
PRIMER_RIGHT_3_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_3=930,20
PRIMER_RIGHT_3=1512,20
PRIMER_LEFT_3_TM=60.278
PRIMER_RIGHT_3_TM=59.881
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=583
PRIMER_PAIR_4_PENALTY=0.397013
PRIMER_LEFT_4_PENALTY=0.277985
PRIMER_RIGHT_4_PENALTY=0.119028
PRIMER_LEFT_4_SEQUENCE=TGTTGGTGAAAACCAGCAGA
PRIMER_RIGHT_4_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_4=930,20
PRIMER_RIGHT_4=1514,20
PRIMER_LEFT_4_TM=60.278
PRIMER_RIGHT_4_TM=59.881
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=585
PRIMER_PAIR_5_PENALTY=0.571340
PRIMER_LEFT_5_PENALTY=0.452312
PRIMER_RIGHT_5_PENALTY=0.119028
PRIMER_LEFT_5_SEQUENCE=GCGTTGGAATACAATGACGA
PRIMER_RIGHT_5_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_5=877,20
PRIMER_RIGHT_5=1512,20
PRIMER_LEFT_5_TM=59.548
PRIMER_RIGHT_5_TM=59.881
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=636
PRIMER_PAIR_6_PENALTY=0.571340
PRIMER_LEFT_6_PENALTY=0.452312
PRIMER_RIGHT_6_PENALTY=0.119028
PRIMER_LEFT_6_SEQUENCE=GCGTTGGAATACAATGACGA
PRIMER_RIGHT_6_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_6=877,20
PRIMER_RIGHT_6=1514,20
PRIMER_LEFT_6_TM=59.548
PRIMER_RIGHT_6_TM=59.881
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=638
PRIMER_PAIR_7_PENALTY=0.575127
PRIMER_LEFT_7_PENALTY=0.456098
PRIMER_RIGHT_7_PENALTY=0.119028
PRIMER_LEFT_7_SEQUENCE=TTTGGTATGGCATTTTCGCT
PRIMER_RIGHT_7_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_7=554,20
PRIMER_RIGHT_7=1512,20
PRIMER_LEFT_7_TM=60.456
PRIMER_RIGHT_7_TM=59.881
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.9000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=959
PRIMER_PAIR_8_PENALTY=0.575127
PRIMER_LEFT_8_PENALTY=0.456098
PRIMER_RIGHT_8_PENALTY=0.119028
PRIMER_LEFT_8_SEQUENCE=TTTGGTATGGCATTTTCGCT
PRIMER_RIGHT_8_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_8=554,20
PRIMER_RIGHT_8=1514,20
PRIMER_LEFT_8_TM=60.456
PRIMER_RIGHT_8_TM=59.881
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.9000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=961
PRIMER_PAIR_9_PENALTY=0.726906
PRIMER_LEFT_9_PENALTY=0.607877
PRIMER_RIGHT_9_PENALTY=0.119028
PRIMER_LEFT_9_SEQUENCE=TTTTCGCTGGAACTAACCCA
PRIMER_RIGHT_9_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_9=566,20
PRIMER_RIGHT_9=1512,20
PRIMER_LEFT_9_TM=60.608
PRIMER_RIGHT_9_TM=59.881
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=947
PRIMER_PAIR_10_PENALTY=0.726906
PRIMER_LEFT_10_PENALTY=0.607877
PRIMER_RIGHT_10_PENALTY=0.119028
PRIMER_LEFT_10_SEQUENCE=TTTTCGCTGGAACTAACCCA
PRIMER_RIGHT_10_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_10=566,20
PRIMER_RIGHT_10=1514,20
PRIMER_LEFT_10_TM=60.608
PRIMER_RIGHT_10_TM=59.881
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=949
PRIMER_PAIR_11_PENALTY=1.090163
PRIMER_LEFT_11_PENALTY=0.971135
PRIMER_RIGHT_11_PENALTY=0.119028
PRIMER_LEFT_11_SEQUENCE=TATGGCATTTTCGCTGGAAC
PRIMER_RIGHT_11_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_11=559,20
PRIMER_RIGHT_11=1512,20
PRIMER_LEFT_11_TM=60.971
PRIMER_RIGHT_11_TM=59.881
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=954
PRIMER_PAIR_12_PENALTY=1.090163
PRIMER_LEFT_12_PENALTY=0.971135
PRIMER_RIGHT_12_PENALTY=0.119028
PRIMER_LEFT_12_SEQUENCE=TATGGCATTTTCGCTGGAAC
PRIMER_RIGHT_12_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_12=559,20
PRIMER_RIGHT_12=1514,20
PRIMER_LEFT_12_TM=60.971
PRIMER_RIGHT_12_TM=59.881
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=956
PRIMER_PAIR_13_PENALTY=1.094111
PRIMER_LEFT_13_PENALTY=0.975083
PRIMER_RIGHT_13_PENALTY=0.119028
PRIMER_LEFT_13_SEQUENCE=AGGTGCAGTGGAAAGAGCAA
PRIMER_RIGHT_13_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_13=513,20
PRIMER_RIGHT_13=1512,20
PRIMER_LEFT_13_TM=60.975
PRIMER_RIGHT_13_TM=59.881
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=1000
PRIMER_PAIR_14_PENALTY=1.253088
PRIMER_LEFT_14_PENALTY=1.134060
PRIMER_RIGHT_14_PENALTY=0.119028
PRIMER_LEFT_14_SEQUENCE=GGAAGAAGCTCGGGTTTGAC
PRIMER_RIGHT_14_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_14=835,20
PRIMER_RIGHT_14=1512,20
PRIMER_LEFT_14_TM=61.134
PRIMER_RIGHT_14_TM=59.881
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=678
PRIMER_PAIR_15_PENALTY=1.253088
PRIMER_LEFT_15_PENALTY=1.134060
PRIMER_RIGHT_15_PENALTY=0.119028
PRIMER_LEFT_15_SEQUENCE=GGAAGAAGCTCGGGTTTGAC
PRIMER_RIGHT_15_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_15=835,20
PRIMER_RIGHT_15=1514,20
PRIMER_LEFT_15_TM=61.134
PRIMER_RIGHT_15_TM=59.881
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=680
PRIMER_PAIR_16_PENALTY=1.298053
PRIMER_LEFT_16_PENALTY=1.179024
PRIMER_RIGHT_16_PENALTY=0.119028
PRIMER_LEFT_16_SEQUENCE=TTTGACCACTGGGACCATAA
PRIMER_RIGHT_16_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_16=849,20
PRIMER_RIGHT_16=1512,20
PRIMER_LEFT_16_TM=58.821
PRIMER_RIGHT_16_TM=59.881
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.2000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=664
PRIMER_PAIR_17_PENALTY=1.298053
PRIMER_LEFT_17_PENALTY=1.179024
PRIMER_RIGHT_17_PENALTY=0.119028
PRIMER_LEFT_17_SEQUENCE=TTTGACCACTGGGACCATAA
PRIMER_RIGHT_17_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_17=849,20
PRIMER_RIGHT_17=1514,20
PRIMER_LEFT_17_TM=58.821
PRIMER_RIGHT_17_TM=59.881
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.2000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=666
PRIMER_PAIR_18_PENALTY=1.416477
PRIMER_LEFT_18_PENALTY=1.297449
PRIMER_RIGHT_18_PENALTY=0.119028
PRIMER_LEFT_18_SEQUENCE=GTTTGACCACTGGGACCATAA
PRIMER_RIGHT_18_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_18=848,21
PRIMER_RIGHT_18=1512,20
PRIMER_LEFT_18_TM=59.703
PRIMER_RIGHT_18_TM=59.881
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.2000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=665
PRIMER_PAIR_19_PENALTY=1.416477
PRIMER_LEFT_19_PENALTY=1.297449
PRIMER_RIGHT_19_PENALTY=0.119028
PRIMER_LEFT_19_SEQUENCE=GTTTGACCACTGGGACCATAA
PRIMER_RIGHT_19_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_19=848,21
PRIMER_RIGHT_19=1514,20
PRIMER_LEFT_19_TM=59.703
PRIMER_RIGHT_19_TM=59.881
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=667
PRIMER_PAIR_20_PENALTY=1.439075
PRIMER_LEFT_20_PENALTY=0.277985
PRIMER_RIGHT_20_PENALTY=1.161090
PRIMER_LEFT_20_SEQUENCE=TGTTGGTGAAAACCAGCAGA
PRIMER_RIGHT_20_SEQUENCE=ATCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_20=930,20
PRIMER_RIGHT_20=1515,21
PRIMER_LEFT_20_TM=60.278
PRIMER_RIGHT_20_TM=60.161
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=586
PRIMER_PAIR_21_PENALTY=1.613402
PRIMER_LEFT_21_PENALTY=0.452312
PRIMER_RIGHT_21_PENALTY=1.161090
PRIMER_LEFT_21_SEQUENCE=GCGTTGGAATACAATGACGA
PRIMER_RIGHT_21_SEQUENCE=ATCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_21=877,20
PRIMER_RIGHT_21=1515,21
PRIMER_LEFT_21_TM=59.548
PRIMER_RIGHT_21_TM=60.161
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=639
PRIMER_PAIR_22_PENALTY=1.617188
PRIMER_LEFT_22_PENALTY=0.456098
PRIMER_RIGHT_22_PENALTY=1.161090
PRIMER_LEFT_22_SEQUENCE=TTTGGTATGGCATTTTCGCT
PRIMER_RIGHT_22_SEQUENCE=ATCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_22=554,20
PRIMER_RIGHT_22=1515,21
PRIMER_LEFT_22_TM=60.456
PRIMER_RIGHT_22_TM=60.161
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.9000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=962
PRIMER_PAIR_23_PENALTY=1.635690
PRIMER_LEFT_23_PENALTY=1.516662
PRIMER_RIGHT_23_PENALTY=0.119028
PRIMER_LEFT_23_SEQUENCE=GCGTTGGAATACAATGACGAG
PRIMER_RIGHT_23_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_23=877,21
PRIMER_RIGHT_23=1512,20
PRIMER_LEFT_23_TM=60.517
PRIMER_RIGHT_23_TM=59.881
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=636
PRIMER_PAIR_24_PENALTY=1.635690
PRIMER_LEFT_24_PENALTY=1.516662
PRIMER_RIGHT_24_PENALTY=0.119028
PRIMER_LEFT_24_SEQUENCE=GCGTTGGAATACAATGACGAG
PRIMER_RIGHT_24_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_24=877,21
PRIMER_RIGHT_24=1514,20
PRIMER_LEFT_24_TM=60.517
PRIMER_RIGHT_24_TM=59.881
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=638
PRIMER_PAIR_25_PENALTY=1.709303
PRIMER_LEFT_25_PENALTY=1.590274
PRIMER_RIGHT_25_PENALTY=0.119028
PRIMER_LEFT_25_SEQUENCE=GAAGCTCGGGTTTGACCACT
PRIMER_RIGHT_25_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_25=839,20
PRIMER_RIGHT_25=1512,20
PRIMER_LEFT_25_TM=61.590
PRIMER_RIGHT_25_TM=59.881
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=674
PRIMER_PAIR_26_PENALTY=1.709303
PRIMER_LEFT_26_PENALTY=1.590274
PRIMER_RIGHT_26_PENALTY=0.119028
PRIMER_LEFT_26_SEQUENCE=GAAGCTCGGGTTTGACCACT
PRIMER_RIGHT_26_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_26=839,20
PRIMER_RIGHT_26=1514,20
PRIMER_LEFT_26_TM=61.590
PRIMER_RIGHT_26_TM=59.881
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=676
PRIMER_PAIR_27_PENALTY=1.709303
PRIMER_LEFT_27_PENALTY=1.590274
PRIMER_RIGHT_27_PENALTY=0.119028
PRIMER_LEFT_27_SEQUENCE=AGAAGCTCGGGTTTGACCAC
PRIMER_RIGHT_27_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_27=838,20
PRIMER_RIGHT_27=1512,20
PRIMER_LEFT_27_TM=61.590
PRIMER_RIGHT_27_TM=59.881
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=675
PRIMER_PAIR_28_PENALTY=1.709303
PRIMER_LEFT_28_PENALTY=1.590274
PRIMER_RIGHT_28_PENALTY=0.119028
PRIMER_LEFT_28_SEQUENCE=AGAAGCTCGGGTTTGACCAC
PRIMER_RIGHT_28_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_28=838,20
PRIMER_RIGHT_28=1514,20
PRIMER_LEFT_28_TM=61.590
PRIMER_RIGHT_28_TM=59.881
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=677
PRIMER_PAIR_29_PENALTY=1.768968
PRIMER_LEFT_29_PENALTY=0.607877
PRIMER_RIGHT_29_PENALTY=1.161090
PRIMER_LEFT_29_SEQUENCE=TTTTCGCTGGAACTAACCCA
PRIMER_RIGHT_29_SEQUENCE=ATCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_29=566,20
PRIMER_RIGHT_29=1515,21
PRIMER_LEFT_29_TM=60.608
PRIMER_RIGHT_29_TM=60.161
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=950
PRIMER_PAIR_30_PENALTY=1.868857
PRIMER_LEFT_30_PENALTY=0.246726
PRIMER_RIGHT_30_PENALTY=1.622131
PRIMER_LEFT_30_SEQUENCE=AAGTTGGAAGAAGCTCGGGT
PRIMER_RIGHT_30_SEQUENCE=CCGTTGTTGCTTGAACTTGG
PRIMER_LEFT_30=830,20
PRIMER_RIGHT_30=1513,20
PRIMER_LEFT_30_TM=60.247
PRIMER_RIGHT_30_TM=61.622
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=11.1000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=684
PRIMER_PAIR_31_PENALTY=1.899983
PRIMER_LEFT_31_PENALTY=1.780954
PRIMER_RIGHT_31_PENALTY=0.119028
PRIMER_LEFT_31_SEQUENCE=CATTTGTTCGAGCTGATGGAG
PRIMER_RIGHT_31_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_31=674,21
PRIMER_RIGHT_31=1512,20
PRIMER_LEFT_31_TM=60.781
PRIMER_RIGHT_31_TM=59.881
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=839
PRIMER_PAIR_32_PENALTY=1.899983
PRIMER_LEFT_32_PENALTY=1.780954
PRIMER_RIGHT_32_PENALTY=0.119028
PRIMER_LEFT_32_SEQUENCE=CATTTGTTCGAGCTGATGGAG
PRIMER_RIGHT_32_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_32=674,21
PRIMER_RIGHT_32=1514,20
PRIMER_LEFT_32_TM=60.781
PRIMER_RIGHT_32_TM=59.881
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=841
PRIMER_PAIR_33_PENALTY=1.900116
PRIMER_LEFT_33_PENALTY=0.277985
PRIMER_RIGHT_33_PENALTY=1.622131
PRIMER_LEFT_33_SEQUENCE=TGTTGGTGAAAACCAGCAGA
PRIMER_RIGHT_33_SEQUENCE=CCGTTGTTGCTTGAACTTGG
PRIMER_LEFT_33=930,20
PRIMER_RIGHT_33=1513,20
PRIMER_LEFT_33_TM=60.278
PRIMER_RIGHT_33_TM=61.622
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=584
PRIMER_PAIR_34_PENALTY=1.931503
PRIMER_LEFT_34_PENALTY=1.812474
PRIMER_RIGHT_34_PENALTY=0.119028
PRIMER_LEFT_34_SEQUENCE=ATTTTCGCTGGAACTAACCC
PRIMER_RIGHT_34_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_34=565,20
PRIMER_RIGHT_34=1512,20
PRIMER_LEFT_34_TM=58.188
PRIMER_RIGHT_34_TM=59.881
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=948
PRIMER_PAIR_35_PENALTY=1.931503
PRIMER_LEFT_35_PENALTY=1.812474
PRIMER_RIGHT_35_PENALTY=0.119028
PRIMER_LEFT_35_SEQUENCE=ATTTTCGCTGGAACTAACCC
PRIMER_RIGHT_35_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_35=565,20
PRIMER_RIGHT_35=1514,20
PRIMER_LEFT_35_TM=58.188
PRIMER_RIGHT_35_TM=59.881
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=950
PRIMER_PAIR_36_PENALTY=1.954088
PRIMER_LEFT_36_PENALTY=1.835060
PRIMER_RIGHT_36_PENALTY=0.119028
PRIMER_LEFT_36_SEQUENCE=ATTTTCGCTGGAACTAACCCA
PRIMER_RIGHT_36_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_36=565,21
PRIMER_RIGHT_36=1512,20
PRIMER_LEFT_36_TM=60.835
PRIMER_RIGHT_36_TM=59.881
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=948
PRIMER_PAIR_37_PENALTY=1.954088
PRIMER_LEFT_37_PENALTY=1.835060
PRIMER_RIGHT_37_PENALTY=0.119028
PRIMER_LEFT_37_SEQUENCE=ATTTTCGCTGGAACTAACCCA
PRIMER_RIGHT_37_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_37=565,21
PRIMER_RIGHT_37=1514,20
PRIMER_LEFT_37_TM=60.835
PRIMER_RIGHT_37_TM=59.881
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=950
PRIMER_PAIR_38_PENALTY=1.954088
PRIMER_LEFT_38_PENALTY=1.835060
PRIMER_RIGHT_38_PENALTY=0.119028
PRIMER_LEFT_38_SEQUENCE=CATTTTCGCTGGAACTAACCC
PRIMER_RIGHT_38_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_38=564,21
PRIMER_RIGHT_38=1512,20
PRIMER_LEFT_38_TM=60.835
PRIMER_RIGHT_38_TM=59.881
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=949
PRIMER_PAIR_39_PENALTY=1.954088
PRIMER_LEFT_39_PENALTY=1.835060
PRIMER_RIGHT_39_PENALTY=0.119028
PRIMER_LEFT_39_SEQUENCE=CATTTTCGCTGGAACTAACCC
PRIMER_RIGHT_39_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_39=564,21
PRIMER_RIGHT_39=1514,20
PRIMER_LEFT_39_TM=60.835
PRIMER_RIGHT_39_TM=59.881
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=951
PRIMER_PAIR_40_PENALTY=2.018682
PRIMER_LEFT_40_PENALTY=1.899654
PRIMER_RIGHT_40_PENALTY=0.119028
PRIMER_LEFT_40_SEQUENCE=TTGGTATGGCATTTTCGCTG
PRIMER_RIGHT_40_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_40=555,20
PRIMER_RIGHT_40=1512,20
PRIMER_LEFT_40_TM=61.900
PRIMER_RIGHT_40_TM=59.881
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=10.2000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=958
PRIMER_PAIR_41_PENALTY=2.018682
PRIMER_LEFT_41_PENALTY=1.899654
PRIMER_RIGHT_41_PENALTY=0.119028
PRIMER_LEFT_41_SEQUENCE=TTGGTATGGCATTTTCGCTG
PRIMER_RIGHT_41_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_41=555,20
PRIMER_RIGHT_41=1514,20
PRIMER_LEFT_41_TM=61.900
PRIMER_RIGHT_41_TM=59.881
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=10.2000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=960
PRIMER_PAIR_42_PENALTY=2.022129
PRIMER_LEFT_42_PENALTY=0.013872
PRIMER_RIGHT_42_PENALTY=2.008257
PRIMER_LEFT_42_SEQUENCE=CAAAGTTGGAAGAAGCTCGG
PRIMER_RIGHT_42_SEQUENCE=TTGAACTTGGAATGAACTGGTG
PRIMER_LEFT_42=828,20
PRIMER_RIGHT_42=1503,22
PRIMER_LEFT_42_TM=59.986
PRIMER_RIGHT_42_TM=60.008
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=676
PRIMER_PAIR_43_PENALTY=2.032009
PRIMER_LEFT_43_PENALTY=1.912980
PRIMER_RIGHT_43_PENALTY=0.119028
PRIMER_LEFT_43_SEQUENCE=GTGCAGTGGAAAGAGCAACT
PRIMER_RIGHT_43_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_43=515,20
PRIMER_RIGHT_43=1512,20
PRIMER_LEFT_43_TM=58.087
PRIMER_RIGHT_43_TM=59.881
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=998
PRIMER_PAIR_44_PENALTY=2.032009
PRIMER_LEFT_44_PENALTY=1.912980
PRIMER_RIGHT_44_PENALTY=0.119028
PRIMER_LEFT_44_SEQUENCE=GTGCAGTGGAAAGAGCAACT
PRIMER_RIGHT_44_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_44=515,20
PRIMER_RIGHT_44=1514,20
PRIMER_LEFT_44_TM=58.087
PRIMER_RIGHT_44_TM=59.881
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=1000
PRIMER_PAIR_45_PENALTY=2.074443
PRIMER_LEFT_45_PENALTY=0.452312
PRIMER_RIGHT_45_PENALTY=1.622131
PRIMER_LEFT_45_SEQUENCE=GCGTTGGAATACAATGACGA
PRIMER_RIGHT_45_SEQUENCE=CCGTTGTTGCTTGAACTTGG
PRIMER_LEFT_45=877,20
PRIMER_RIGHT_45=1513,20
PRIMER_LEFT_45_TM=59.548
PRIMER_RIGHT_45_TM=61.622
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=637
PRIMER_PAIR_46_PENALTY=2.078229
PRIMER_LEFT_46_PENALTY=0.456098
PRIMER_RIGHT_46_PENALTY=1.622131
PRIMER_LEFT_46_SEQUENCE=TTTGGTATGGCATTTTCGCT
PRIMER_RIGHT_46_SEQUENCE=CCGTTGTTGCTTGAACTTGG
PRIMER_LEFT_46=554,20
PRIMER_RIGHT_46=1513,20
PRIMER_LEFT_46_TM=60.456
PRIMER_RIGHT_46_TM=61.622
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=960
PRIMER_PAIR_47_PENALTY=2.090861
PRIMER_LEFT_47_PENALTY=1.971833
PRIMER_RIGHT_47_PENALTY=0.119028
PRIMER_LEFT_47_SEQUENCE=GCATTTTCGCTGGAACTAACC
PRIMER_RIGHT_47_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_47=563,21
PRIMER_RIGHT_47=1512,20
PRIMER_LEFT_47_TM=60.972
PRIMER_RIGHT_47_TM=59.881
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.2000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=950
PRIMER_PAIR_48_PENALTY=2.090861
PRIMER_LEFT_48_PENALTY=1.971833
PRIMER_RIGHT_48_PENALTY=0.119028
PRIMER_LEFT_48_SEQUENCE=GCATTTTCGCTGGAACTAACC
PRIMER_RIGHT_48_SEQUENCE=TCCGTTGTTGCTTGAACTTG
PRIMER_LEFT_48=563,21
PRIMER_RIGHT_48=1514,20
PRIMER_LEFT_48_TM=60.972
PRIMER_RIGHT_48_TM=59.881
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.2000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=952
PRIMER_PAIR_49_PENALTY=2.090861
PRIMER_LEFT_49_PENALTY=1.971833
PRIMER_RIGHT_49_PENALTY=0.119028
PRIMER_LEFT_49_SEQUENCE=GGCATTTTCGCTGGAACTAAC
PRIMER_RIGHT_49_SEQUENCE=CGTTGTTGCTTGAACTTGGA
PRIMER_LEFT_49=562,21
PRIMER_RIGHT_49=1512,20
PRIMER_LEFT_49_TM=60.972
PRIMER_RIGHT_49_TM=59.881
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=5.7000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=951
=
SEQUENCE_ID=MAGIC.338_1_22753079_22753208_can-0_edi-0_164_902170_TRUE
SEQUENCE_TARGET=950,229
SEQUENCE_TEMPLATE=ATAAGCCGTGTGTCTTTGTNATACTCGNAANCTTAAANNATATTAGAATCAANGTGATTTATTTATAGNAAAAGGAATAAGGCTTTTAAGCTTCAATCAAACGAAGCTTATAGCTGAAGATATTAAGAACTTGTTGTATTCACATAAAGCAAAAAAGANGAAACTTGTTCGTCCTTTATTGTACATCTNAAAGACTAAANATTTATGCTCANAATGTTTTCTTTTGCCTTTTATATTCATATTACATTATACATGATCCTCCTTTCTTTTGGCGCATGGCATATTTTGTCAAACTCTATTACAACATTGATTCTAATCGATCTACAGTTGAAACTAGTGAATCGAATTNTCAGGTAGAAGTTAAATTTGATTTGANATGTNNTCAAGTTTATGCTANCTTTCTCATATAATATCCAATATTTTAGACNAGTTATATGTTGTTTATGTCGAAGATTNTTATTTGTTAGGTACGANAAATTTATATGAGCCACCANATATATAGTTGTAATCGACCATTTTCTNTAATTTNTTGGTTAAGATTTAAGAAGACTAGAAGAGGGACCGATGTCACTAGATNCACAAACTTAGCNACNTANNTTTACATGAATCCAAGTTACATATTAAACCAATGNCAAGAGCCGAAGCACCTCATGAATCCAAAAAATCTACGTAGGACGGTAACTTTTGTTGATCNTGAGACAAAGGATNTAGCCGAATATGANTNGCAACCAANTNTTAACTTCTCTCTATCAACGACGATAATCTTNGTTGCAACATGAAAAACATTCATAAACAAACATTTGCCGGTTCTCCTTTAGTCGCGGAATGATCTCTTTCCNATGGCCAGAGACATGTTGTGGTAANCNAGCCTAGTANTTCNNNNNTTTATGGTTCCGAGAACNATNTTTGNNNNTNTNGANTNACCNNTCCANNNNTTNANTNTNNANNNTNANTCNATNTTCACTNTTTNNANTNTNGAATCNTGANTCNANNCNATGNTNTNNNNNTTTGTTTGTACTTTGGAGAAAACCAACNNNNATTTCCAATAGGGCATAATTTGAGATNNNNNTNTTNANTNATAAATACGTACTAATCATGCATGGGACTTNTTGAGTATTTTATCGATATTNAGATCTNANGTANNNATTCTGTTCTTGCATTGCATNTATTTTTNAGTGGCATTTGATAGATTAGACACACATATATGTCTTTATAGTAANATATGAATAATNTTTTTTCTGTAAACATNAATGATGTTGAATNCAAATNAGTATATATTTATCGGTATCTATACTAAAATCATATATGTATATTCAAATCATAAAGTAGGAAGCAGTGTGGGCCGCTATTCTACTGGCTTCCACTNCTGAATTTCCATAATATATAGTTCTTAATCGAACCAACAANAAGTTGANTCCNNATGGANACATATTTATACATGGATATGTACGAACATAAATACATGCATGTANNCAGAAAATGNNNNNNNNTGCAAGTTTGGACATAANNNNNNNNATGTAANAGTAACGGCACTATCACNAACAATTAGGTTNTAAGTGTTGNTTTTNTGATTGTTAAANTTANAATTTATNATTNANNATGAAAAAAACAANTNTATAAAAACAACNAAGAACTTCATATGACAAACCACCAATTATTATGTTCTCTAACTTTAATAAGTGANTAACAAAGATGTTACCAACGACAAAAATATGNNNNTATATATATTCAAGAACAATGACAGGAACAAAAGGTTNTTCAACATAAAAACAACCCATATATGCTAAATTCAGCAATAAACCCCCCTTAAAATNTTNAAAGAAGAAGAAAATACATTNAAAATTATTGTTATGCTTTAGAGTCTCCAAGCTGAATTTAGCATATGTTCCCANCCGTCTTGACAACCACAACCCTCTCTCTTTAATCACCTATATATAATTAAAAAGTGAAATTTAGGTTAATNATTACATATNTAACATGAGAGTATGTAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTATCCCAAANTTTCTAAGCCCTAAATCATTCTTGCTTTNGTAGTTGGTCCCCTCCAACACTATNGNTGCCTCTGCCCACCTTTGAAGAGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.665153
PRIMER_LEFT_0_PENALTY=0.022341
PRIMER_RIGHT_0_PENALTY=0.642812
PRIMER_LEFT_0_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_0_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_0=792,20
PRIMER_RIGHT_0=1342,20
PRIMER_LEFT_0_TM=59.978
PRIMER_RIGHT_0_TM=59.357
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=5.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=551
PRIMER_PAIR_1_PENALTY=0.797691
PRIMER_LEFT_1_PENALTY=0.154878
PRIMER_RIGHT_1_PENALTY=0.642812
PRIMER_LEFT_1_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_1_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_1=790,20
PRIMER_RIGHT_1=1342,20
PRIMER_LEFT_1_TM=59.845
PRIMER_RIGHT_1_TM=59.357
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=553
PRIMER_PAIR_2_PENALTY=0.799921
PRIMER_LEFT_2_PENALTY=0.022341
PRIMER_RIGHT_2_PENALTY=0.777580
PRIMER_LEFT_2_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_2_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_2=792,20
PRIMER_RIGHT_2=1353,20
PRIMER_LEFT_2_TM=59.978
PRIMER_RIGHT_2_TM=59.222
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=562
PRIMER_PAIR_3_PENALTY=0.799921
PRIMER_LEFT_3_PENALTY=0.022341
PRIMER_RIGHT_3_PENALTY=0.777580
PRIMER_LEFT_3_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_3_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_3=792,20
PRIMER_RIGHT_3=1354,20
PRIMER_LEFT_3_TM=59.978
PRIMER_RIGHT_3_TM=59.222
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=563
PRIMER_PAIR_4_PENALTY=0.932458
PRIMER_LEFT_4_PENALTY=0.154878
PRIMER_RIGHT_4_PENALTY=0.777580
PRIMER_LEFT_4_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_4_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_4=790,20
PRIMER_RIGHT_4=1353,20
PRIMER_LEFT_4_TM=59.845
PRIMER_RIGHT_4_TM=59.222
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=564
PRIMER_PAIR_5_PENALTY=0.932458
PRIMER_LEFT_5_PENALTY=0.154878
PRIMER_RIGHT_5_PENALTY=0.777580
PRIMER_LEFT_5_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_5_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_5=790,20
PRIMER_RIGHT_5=1354,20
PRIMER_LEFT_5_TM=59.845
PRIMER_RIGHT_5_TM=59.222
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=565
PRIMER_PAIR_6_PENALTY=0.986762
PRIMER_LEFT_6_PENALTY=0.343950
PRIMER_RIGHT_6_PENALTY=0.642812
PRIMER_LEFT_6_SEQUENCE=TCTCCTTTAGTCGCGGAATG
PRIMER_RIGHT_6_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_6=808,20
PRIMER_RIGHT_6=1342,20
PRIMER_LEFT_6_TM=60.344
PRIMER_RIGHT_6_TM=59.357
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=535
PRIMER_PAIR_7_PENALTY=1.070526
PRIMER_LEFT_7_PENALTY=0.022341
PRIMER_RIGHT_7_PENALTY=1.048185
PRIMER_LEFT_7_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_7_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_7=792,20
PRIMER_RIGHT_7=1361,20
PRIMER_LEFT_7_TM=59.978
PRIMER_RIGHT_7_TM=58.952
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=11.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=570
PRIMER_PAIR_8_PENALTY=1.121529
PRIMER_LEFT_8_PENALTY=0.343950
PRIMER_RIGHT_8_PENALTY=0.777580
PRIMER_LEFT_8_SEQUENCE=TCTCCTTTAGTCGCGGAATG
PRIMER_RIGHT_8_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_8=808,20
PRIMER_RIGHT_8=1353,20
PRIMER_LEFT_8_TM=60.344
PRIMER_RIGHT_8_TM=59.222
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=6.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=546
PRIMER_PAIR_9_PENALTY=1.121529
PRIMER_LEFT_9_PENALTY=0.343950
PRIMER_RIGHT_9_PENALTY=0.777580
PRIMER_LEFT_9_SEQUENCE=TCTCCTTTAGTCGCGGAATG
PRIMER_RIGHT_9_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_9=808,20
PRIMER_RIGHT_9=1354,20
PRIMER_LEFT_9_TM=60.344
PRIMER_RIGHT_9_TM=59.222
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=547
PRIMER_PAIR_10_PENALTY=1.167122
PRIMER_LEFT_10_PENALTY=0.524309
PRIMER_RIGHT_10_PENALTY=0.642812
PRIMER_LEFT_10_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_10_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_10=791,20
PRIMER_RIGHT_10=1342,20
PRIMER_LEFT_10_TM=59.476
PRIMER_RIGHT_10_TM=59.357
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=5.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=552
PRIMER_PAIR_11_PENALTY=1.187388
PRIMER_LEFT_11_PENALTY=0.022341
PRIMER_RIGHT_11_PENALTY=1.165047
PRIMER_LEFT_11_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_11_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_11=792,20
PRIMER_RIGHT_11=1354,21
PRIMER_LEFT_11_TM=59.978
PRIMER_RIGHT_11_TM=60.165
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=563
PRIMER_PAIR_12_PENALTY=1.190320
PRIMER_LEFT_12_PENALTY=0.547508
PRIMER_RIGHT_12_PENALTY=0.642812
PRIMER_LEFT_12_SEQUENCE=GTTCTCCTTTAGTCGCGGAA
PRIMER_RIGHT_12_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_12=806,20
PRIMER_RIGHT_12=1342,20
PRIMER_LEFT_12_TM=59.452
PRIMER_RIGHT_12_TM=59.357
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.2000
PRIMER_RIGHT_12_END_STABILITY=5.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=537
PRIMER_PAIR_13_PENALTY=1.198616
PRIMER_LEFT_13_PENALTY=0.022341
PRIMER_RIGHT_13_PENALTY=1.176275
PRIMER_LEFT_13_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_13_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_13=792,20
PRIMER_RIGHT_13=1355,20
PRIMER_LEFT_13_TM=59.978
PRIMER_RIGHT_13_TM=61.176
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=564
PRIMER_PAIR_14_PENALTY=1.203064
PRIMER_LEFT_14_PENALTY=0.154878
PRIMER_RIGHT_14_PENALTY=1.048185
PRIMER_LEFT_14_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_14_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_14=790,20
PRIMER_RIGHT_14=1361,20
PRIMER_LEFT_14_TM=59.845
PRIMER_RIGHT_14_TM=58.952
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=11.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=572
PRIMER_PAIR_15_PENALTY=1.301889
PRIMER_LEFT_15_PENALTY=0.524309
PRIMER_RIGHT_15_PENALTY=0.777580
PRIMER_LEFT_15_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_15_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_15=791,20
PRIMER_RIGHT_15=1353,20
PRIMER_LEFT_15_TM=59.476
PRIMER_RIGHT_15_TM=59.222
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=563
PRIMER_PAIR_16_PENALTY=1.301889
PRIMER_LEFT_16_PENALTY=0.524309
PRIMER_RIGHT_16_PENALTY=0.777580
PRIMER_LEFT_16_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_16_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_16=791,20
PRIMER_RIGHT_16=1354,20
PRIMER_LEFT_16_TM=59.476
PRIMER_RIGHT_16_TM=59.222
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=564
PRIMER_PAIR_17_PENALTY=1.319926
PRIMER_LEFT_17_PENALTY=0.154878
PRIMER_RIGHT_17_PENALTY=1.165047
PRIMER_LEFT_17_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_17_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_17=790,20
PRIMER_RIGHT_17=1354,21
PRIMER_LEFT_17_TM=59.845
PRIMER_RIGHT_17_TM=60.165
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.1000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=565
PRIMER_PAIR_18_PENALTY=1.325087
PRIMER_LEFT_18_PENALTY=0.547508
PRIMER_RIGHT_18_PENALTY=0.777580
PRIMER_LEFT_18_SEQUENCE=GTTCTCCTTTAGTCGCGGAA
PRIMER_RIGHT_18_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_18=806,20
PRIMER_RIGHT_18=1353,20
PRIMER_LEFT_18_TM=59.452
PRIMER_RIGHT_18_TM=59.222
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=10.2000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=548
PRIMER_PAIR_19_PENALTY=1.325087
PRIMER_LEFT_19_PENALTY=0.547508
PRIMER_RIGHT_19_PENALTY=0.777580
PRIMER_LEFT_19_SEQUENCE=GTTCTCCTTTAGTCGCGGAA
PRIMER_RIGHT_19_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_19=806,20
PRIMER_RIGHT_19=1354,20
PRIMER_LEFT_19_TM=59.452
PRIMER_RIGHT_19_TM=59.222
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=10.2000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=549
PRIMER_PAIR_20_PENALTY=1.330776
PRIMER_LEFT_20_PENALTY=0.687963
PRIMER_RIGHT_20_PENALTY=0.642812
PRIMER_LEFT_20_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_20_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_20=800,20
PRIMER_RIGHT_20=1342,20
PRIMER_LEFT_20_TM=59.312
PRIMER_RIGHT_20_TM=59.357
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=5.4000
PRIMER_RIGHT_20_END_STABILITY=5.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=543
PRIMER_PAIR_21_PENALTY=1.331154
PRIMER_LEFT_21_PENALTY=0.154878
PRIMER_RIGHT_21_PENALTY=1.176275
PRIMER_LEFT_21_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_21_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_21=790,20
PRIMER_RIGHT_21=1355,20
PRIMER_LEFT_21_TM=59.845
PRIMER_RIGHT_21_TM=61.176
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=566
PRIMER_PAIR_22_PENALTY=1.392135
PRIMER_LEFT_22_PENALTY=0.343950
PRIMER_RIGHT_22_PENALTY=1.048185
PRIMER_LEFT_22_SEQUENCE=TCTCCTTTAGTCGCGGAATG
PRIMER_RIGHT_22_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_22=808,20
PRIMER_RIGHT_22=1361,20
PRIMER_LEFT_22_TM=60.344
PRIMER_RIGHT_22_TM=58.952
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.9000
PRIMER_RIGHT_22_END_STABILITY=11.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=554
PRIMER_PAIR_23_PENALTY=1.465543
PRIMER_LEFT_23_PENALTY=0.687963
PRIMER_RIGHT_23_PENALTY=0.777580
PRIMER_LEFT_23_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_23_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_23=800,20
PRIMER_RIGHT_23=1353,20
PRIMER_LEFT_23_TM=59.312
PRIMER_RIGHT_23_TM=59.222
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=5.4000
PRIMER_RIGHT_23_END_STABILITY=6.1000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=554
PRIMER_PAIR_24_PENALTY=1.465543
PRIMER_LEFT_24_PENALTY=0.687963
PRIMER_RIGHT_24_PENALTY=0.777580
PRIMER_LEFT_24_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_24_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_24=800,20
PRIMER_RIGHT_24=1354,20
PRIMER_LEFT_24_TM=59.312
PRIMER_RIGHT_24_TM=59.222
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=5.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=555
PRIMER_PAIR_25_PENALTY=1.498434
PRIMER_LEFT_25_PENALTY=0.855622
PRIMER_RIGHT_25_PENALTY=0.642812
PRIMER_LEFT_25_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_25_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_25=796,20
PRIMER_RIGHT_25=1342,20
PRIMER_LEFT_25_TM=60.856
PRIMER_RIGHT_25_TM=59.357
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=5.7000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=547
PRIMER_PAIR_26_PENALTY=1.498434
PRIMER_LEFT_26_PENALTY=0.855622
PRIMER_RIGHT_26_PENALTY=0.642812
PRIMER_LEFT_26_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_26_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_26=795,20
PRIMER_RIGHT_26=1342,20
PRIMER_LEFT_26_TM=60.856
PRIMER_RIGHT_26_TM=59.357
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=5.7000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=548
PRIMER_PAIR_27_PENALTY=1.498434
PRIMER_LEFT_27_PENALTY=0.855622
PRIMER_RIGHT_27_PENALTY=0.642812
PRIMER_LEFT_27_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_27_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_27=794,20
PRIMER_RIGHT_27=1342,20
PRIMER_LEFT_27_TM=60.856
PRIMER_RIGHT_27_TM=59.357
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=5.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=549
PRIMER_PAIR_28_PENALTY=1.508997
PRIMER_LEFT_28_PENALTY=0.343950
PRIMER_RIGHT_28_PENALTY=1.165047
PRIMER_LEFT_28_SEQUENCE=TCTCCTTTAGTCGCGGAATG
PRIMER_RIGHT_28_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_28=808,20
PRIMER_RIGHT_28=1354,21
PRIMER_LEFT_28_TM=60.344
PRIMER_RIGHT_28_TM=60.165
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=547
PRIMER_PAIR_29_PENALTY=1.520225
PRIMER_LEFT_29_PENALTY=0.343950
PRIMER_RIGHT_29_PENALTY=1.176275
PRIMER_LEFT_29_SEQUENCE=TCTCCTTTAGTCGCGGAATG
PRIMER_RIGHT_29_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_29=808,20
PRIMER_RIGHT_29=1355,20
PRIMER_LEFT_29_TM=60.344
PRIMER_RIGHT_29_TM=61.176
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=548
PRIMER_PAIR_30_PENALTY=1.572494
PRIMER_LEFT_30_PENALTY=0.524309
PRIMER_RIGHT_30_PENALTY=1.048185
PRIMER_LEFT_30_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_30_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_30=791,20
PRIMER_RIGHT_30=1361,20
PRIMER_LEFT_30_TM=59.476
PRIMER_RIGHT_30_TM=58.952
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=11.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=571
PRIMER_PAIR_31_PENALTY=1.595693
PRIMER_LEFT_31_PENALTY=0.547508
PRIMER_RIGHT_31_PENALTY=1.048185
PRIMER_LEFT_31_SEQUENCE=GTTCTCCTTTAGTCGCGGAA
PRIMER_RIGHT_31_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_31=806,20
PRIMER_RIGHT_31=1361,20
PRIMER_LEFT_31_TM=59.452
PRIMER_RIGHT_31_TM=58.952
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=10.2000
PRIMER_RIGHT_31_END_STABILITY=11.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=556
PRIMER_PAIR_32_PENALTY=1.631074
PRIMER_LEFT_32_PENALTY=0.022341
PRIMER_RIGHT_32_PENALTY=1.608733
PRIMER_LEFT_32_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_32_SEQUENCE=GGCCCACACTGCTTCCTACT
PRIMER_LEFT_32=792,20
PRIMER_RIGHT_32=1343,20
PRIMER_LEFT_32_TM=59.978
PRIMER_RIGHT_32_TM=61.609
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=60.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=5.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=552
PRIMER_PAIR_33_PENALTY=1.633201
PRIMER_LEFT_33_PENALTY=0.855622
PRIMER_RIGHT_33_PENALTY=0.777580
PRIMER_LEFT_33_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_33_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_33=796,20
PRIMER_RIGHT_33=1353,20
PRIMER_LEFT_33_TM=60.856
PRIMER_RIGHT_33_TM=59.222
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.1000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=558
PRIMER_PAIR_34_PENALTY=1.633201
PRIMER_LEFT_34_PENALTY=0.855622
PRIMER_RIGHT_34_PENALTY=0.777580
PRIMER_LEFT_34_SEQUENCE=ACATTTGCCGGTTCTCCTTT
PRIMER_RIGHT_34_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_34=796,20
PRIMER_RIGHT_34=1354,20
PRIMER_LEFT_34_TM=60.856
PRIMER_RIGHT_34_TM=59.222
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=559
PRIMER_PAIR_35_PENALTY=1.633201
PRIMER_LEFT_35_PENALTY=0.855622
PRIMER_RIGHT_35_PENALTY=0.777580
PRIMER_LEFT_35_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_35_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_35=795,20
PRIMER_RIGHT_35=1353,20
PRIMER_LEFT_35_TM=60.856
PRIMER_RIGHT_35_TM=59.222
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=559
PRIMER_PAIR_36_PENALTY=1.633201
PRIMER_LEFT_36_PENALTY=0.855622
PRIMER_RIGHT_36_PENALTY=0.777580
PRIMER_LEFT_36_SEQUENCE=AACATTTGCCGGTTCTCCTT
PRIMER_RIGHT_36_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_36=795,20
PRIMER_RIGHT_36=1354,20
PRIMER_LEFT_36_TM=60.856
PRIMER_RIGHT_36_TM=59.222
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=560
PRIMER_PAIR_37_PENALTY=1.633201
PRIMER_LEFT_37_PENALTY=0.855622
PRIMER_RIGHT_37_PENALTY=0.777580
PRIMER_LEFT_37_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_37_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_37=794,20
PRIMER_RIGHT_37=1353,20
PRIMER_LEFT_37_TM=60.856
PRIMER_RIGHT_37_TM=59.222
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.1000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=560
PRIMER_PAIR_38_PENALTY=1.633201
PRIMER_LEFT_38_PENALTY=0.855622
PRIMER_RIGHT_38_PENALTY=0.777580
PRIMER_LEFT_38_SEQUENCE=AAACATTTGCCGGTTCTCCT
PRIMER_RIGHT_38_SEQUENCE=AGTAGAATAGCGGCCCACAC
PRIMER_LEFT_38=794,20
PRIMER_RIGHT_38=1354,20
PRIMER_LEFT_38_TM=60.856
PRIMER_RIGHT_38_TM=59.222
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=561
PRIMER_PAIR_39_PENALTY=1.689356
PRIMER_LEFT_39_PENALTY=0.524309
PRIMER_RIGHT_39_PENALTY=1.165047
PRIMER_LEFT_39_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_39_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_39=791,20
PRIMER_RIGHT_39=1354,21
PRIMER_LEFT_39_TM=59.476
PRIMER_RIGHT_39_TM=60.165
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=564
PRIMER_PAIR_40_PENALTY=1.697565
PRIMER_LEFT_40_PENALTY=0.022341
PRIMER_RIGHT_40_PENALTY=1.675224
PRIMER_LEFT_40_SEQUENCE=ACAAACATTTGCCGGTTCTC
PRIMER_RIGHT_40_SEQUENCE=GCCCACACTGCTTCCTACTTT
PRIMER_LEFT_40=792,20
PRIMER_RIGHT_40=1342,21
PRIMER_LEFT_40_TM=59.978
PRIMER_RIGHT_40_TM=60.675
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=551
PRIMER_PAIR_41_PENALTY=1.700585
PRIMER_LEFT_41_PENALTY=0.524309
PRIMER_RIGHT_41_PENALTY=1.176275
PRIMER_LEFT_41_SEQUENCE=AACAAACATTTGCCGGTTCT
PRIMER_RIGHT_41_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_41=791,20
PRIMER_RIGHT_41=1355,20
PRIMER_LEFT_41_TM=59.476
PRIMER_RIGHT_41_TM=61.176
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=565
PRIMER_PAIR_42_PENALTY=1.708955
PRIMER_LEFT_42_PENALTY=1.066143
PRIMER_RIGHT_42_PENALTY=0.642812
PRIMER_LEFT_42_SEQUENCE=GAAGCACCTCATGAATCCAAA
PRIMER_RIGHT_42_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_42=640,21
PRIMER_RIGHT_42=1342,20
PRIMER_LEFT_42_TM=60.066
PRIMER_RIGHT_42_TM=59.357
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.8000
PRIMER_RIGHT_42_END_STABILITY=5.7000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=703
PRIMER_PAIR_43_PENALTY=1.712555
PRIMER_LEFT_43_PENALTY=0.547508
PRIMER_RIGHT_43_PENALTY=1.165047
PRIMER_LEFT_43_SEQUENCE=GTTCTCCTTTAGTCGCGGAA
PRIMER_RIGHT_43_SEQUENCE=AGTAGAATAGCGGCCCACACT
PRIMER_LEFT_43=806,20
PRIMER_RIGHT_43=1354,21
PRIMER_LEFT_43_TM=59.452
PRIMER_RIGHT_43_TM=60.165
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=10.2000
PRIMER_RIGHT_43_END_STABILITY=6.1000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=549
PRIMER_PAIR_44_PENALTY=1.723783
PRIMER_LEFT_44_PENALTY=0.547508
PRIMER_RIGHT_44_PENALTY=1.176275
PRIMER_LEFT_44_SEQUENCE=GTTCTCCTTTAGTCGCGGAA
PRIMER_RIGHT_44_SEQUENCE=CAGTAGAATAGCGGCCCACA
PRIMER_LEFT_44=806,20
PRIMER_RIGHT_44=1355,20
PRIMER_LEFT_44_TM=59.452
PRIMER_RIGHT_44_TM=61.176
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=10.2000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=550
PRIMER_PAIR_45_PENALTY=1.736149
PRIMER_LEFT_45_PENALTY=0.687963
PRIMER_RIGHT_45_PENALTY=1.048185
PRIMER_LEFT_45_SEQUENCE=TTGCCGGTTCTCCTTTAGTC
PRIMER_RIGHT_45_SEQUENCE=GGAAGCCAGTAGAATAGCGG
PRIMER_LEFT_45=800,20
PRIMER_RIGHT_45=1361,20
PRIMER_LEFT_45_TM=59.312
PRIMER_RIGHT_45_TM=58.952
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=5.4000
PRIMER_RIGHT_45_END_STABILITY=11.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=562
PRIMER_PAIR_46_PENALTY=1.763612
PRIMER_LEFT_46_PENALTY=0.154878
PRIMER_RIGHT_46_PENALTY=1.608733
PRIMER_LEFT_46_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_46_SEQUENCE=GGCCCACACTGCTTCCTACT
PRIMER_LEFT_46=790,20
PRIMER_RIGHT_46=1343,20
PRIMER_LEFT_46_TM=59.845
PRIMER_RIGHT_46_TM=61.609
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=60.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=5.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=554
PRIMER_PAIR_47_PENALTY=1.809002
PRIMER_LEFT_47_PENALTY=1.166190
PRIMER_RIGHT_47_PENALTY=0.642812
PRIMER_LEFT_47_SEQUENCE=TTTGCCGGTTCTCCTTTAGT
PRIMER_RIGHT_47_SEQUENCE=GCCCACACTGCTTCCTACTT
PRIMER_LEFT_47=799,20
PRIMER_RIGHT_47=1342,20
PRIMER_LEFT_47_TM=58.834
PRIMER_RIGHT_47_TM=59.357
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=5.7000
PRIMER_RIGHT_47_END_STABILITY=5.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=544
PRIMER_PAIR_48_PENALTY=1.830103
PRIMER_LEFT_48_PENALTY=0.154878
PRIMER_RIGHT_48_PENALTY=1.675224
PRIMER_LEFT_48_SEQUENCE=AAACAAACATTTGCCGGTTC
PRIMER_RIGHT_48_SEQUENCE=GCCCACACTGCTTCCTACTTT
PRIMER_LEFT_48=790,20
PRIMER_RIGHT_48=1342,21
PRIMER_LEFT_48_TM=59.845
PRIMER_RIGHT_48_TM=60.675
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=553
PRIMER_PAIR_49_PENALTY=1.843723
PRIMER_LEFT_49_PENALTY=1.066143
PRIMER_RIGHT_49_PENALTY=0.777580
PRIMER_LEFT_49_SEQUENCE=GAAGCACCTCATGAATCCAAA
PRIMER_RIGHT_49_SEQUENCE=GTAGAATAGCGGCCCACACT
PRIMER_LEFT_49=640,21
PRIMER_RIGHT_49=1353,20
PRIMER_LEFT_49_TM=60.066
PRIMER_RIGHT_49_TM=59.222
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.8000
PRIMER_RIGHT_49_END_STABILITY=6.1000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=714
=
SEQUENCE_ID=MAGIC.446_5_17392218_17392698_po-0_bur-0_12540_64227_TRUE
SEQUENCE_TARGET=950,580
SEQUENCE_TEMPLATE=TTAATGTTGCTGTCATGGTTGGTGGTTTCCCGAGGAAGGAAGGAATGGAGAGGAAGGATGTTATGTCCAAGAACGTTTCCATTTACAAGTCTCAAGCTTCTGCCTTGGAGAAGCATGCTGCACCAAACTGCAAGGTGATAAGTCTTTTCGAATCGTTCTTATGATCATTATGCAGAAATNAAGCAAGTTAANCTCTTATTTTGTTGTATGNGATATTTCATATTTGCTATCTCTTCGTTTTGTAGGTTCTGGTTGTTGCAAACCCTGCAAACACCAATGCATTGATCCTGAAGGAATTTGCACCATCGATCCCAGAGAAGAACATTACTTGCTTGACCAGGCTTGACCACAACAGGGCATTGGGACAAGTGTCTGAGAGGTTAAGCGTACCAGTNTCTGATGTGAAGAACGTGATTATCTGGGGAAACCACTCNTCAACCCAATACCCAGATGTCAACCANGCTACCGTNAAGACTTCNNNTGGAGAGAAGCCAGTGCGTGAGCTAGTCAAGAATGACGAGTGGTAAAAATCTNTAAAACACCNTTTTAATATCTCAATCTAGTTTCNTGNATGNGATGCTCATNTATGTTAGCAACTNATGTTGTAGGTTGAATGGAGAGTTCATCTCTNCCGTTCAACAACGTGGTGCTGCCATTATCAAGGCCAGGAAGCTNTCTAGTGCACTNTCTGCAGCTAGCTCNGCTTGTGACCATATCCGTGATTGGGTTGTTGGAACNCCNGAGGTTCGTCATTACTACTGTTTNATCTCGAATCAATNGGTTNTTTTGTGTACTAANTAAAACTANTATGTTCTTNGANTTGATTTCTCAGGGCACATTTGTTTCAATGGGAGTATACTCAGATGGATCCTACAATGTTCCAGCTGGACTTATCTACTCCTTCCCCGTANCCTGCCGTAATGGAGAGTGGACCATTGTTCAAGGTAAATATATTTCTCGTAACCAAAAGTNNNACACAATGTTCTTAAGAGAATCCTAGNCTAAAGCTGACCCGTGTAACGGAACCGTAAACTGGATTGTCTACTTCTAATCCTAAGTGTGCAAGGCTGGTTCTCGCTGNGATGATTAGCTCATAGAACTAGATATTGGTCTAATATGTGTTGTTGTTATTCACAGGTTTACCGATTGATGACGNATCGAGGAAGAAGATGGATTTGACAGCAGAGGAGTTGAANGAAGAGAAGGACCTNGCTTACTCATGCCTCTCTTAAACCNGTCTTTTCTCAGCTGGTTTAATCAGTGANAGCTTTGTTTCGAATAAAGAAGTTTTGTTATAAAATGAACTTTTAATAACCTCATGAGCTTGAGGTTTAGTTTTCCTAATGTNCCCATGATGTGTCGGTACTGATTTGCTGACCANATATAAACAATCATTTTGATAATTGACCTCTTTTGGTTTNCTTTCGTAACGGGAGAGTGGAGAGTGGAGANNNNACAANNNNNNGANTTTTTTNGTAGANTTTTNNAGATATGGGCCAAAGCTTTGTTGGGTATTCTTTTGGCCCATATNANAAAAATACNAAAGATTTCGCANTTGTGAAATAAANAGCTTAAGAATAGTATTATANAATAAAATCTCGCAATAATATACTCCANGGTTTTTATGCGATTTAGAACAATGGGATAATACTAAATTAATNATTTGAATNNNNNNNNNNNNNTAATTTTACGTAACATTTATTTTTTAATTTACTATTATTATTTAATTATGCTATTTTTACTATTTACCAGTTNATCCAAATATAAAACCTTATTAGCCTATNAAAAAAAGNAAACGTAAAACCCTATTNNATAGNTTTTNNTCTAAAANTAAAACCNTATTCTATGNGTTTTTTCTCCANNNNNTGCAGTCACTTTGATTTNTCTTGTAATTTTATTTNTAAATATANNNTTTTTTCTATAAAAGAGCAAATATAGCNGCNGANGATAATGATTTGTTGGGNNNNNNNNNNNNCTNTTTTTTTTNNTTCTAATTCCTGATGAATTATCGACGGCTTAATTCATGTCCTCTNTTTTTTCCTGGACATTGAATGTTNGTGACAANTCTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAACTCATAGTGCAACTAAANANATCTAAACAAAANATCTAAAAGTCTGACAAAACACAAAAAGAAATCCTAACNAAATNCGATAATTAAGCNNAANAAACAAAAGGAAAATNAAAAAATCTAAACAACAATCTTCTNCTNGATTTTCTCCGGNTCAGTTTCGCCGGAAAGCTCCTCAAGTGATTTCCCATTAGTCTCCGGAATTGTAAACGTGAGAAGC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.197066
PRIMER_LEFT_0_PENALTY=0.308722
PRIMER_RIGHT_0_PENALTY=0.888344
PRIMER_LEFT_0_SEQUENCE=CTCAGGGCACATTTGTTTCA
PRIMER_RIGHT_0_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_0=827,20
PRIMER_RIGHT_0=1644,20
PRIMER_LEFT_0_TM=59.691
PRIMER_RIGHT_0_TM=59.112
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=10.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=818
PRIMER_PAIR_1_PENALTY=1.365494
PRIMER_LEFT_1_PENALTY=0.477150
PRIMER_RIGHT_1_PENALTY=0.888344
PRIMER_LEFT_1_SEQUENCE=CCATTATCAAGGCCAGGAAG
PRIMER_RIGHT_1_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_1=652,20
PRIMER_RIGHT_1=1644,20
PRIMER_LEFT_1_TM=59.523
PRIMER_RIGHT_1_TM=59.112
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=993
PRIMER_PAIR_2_PENALTY=1.641396
PRIMER_LEFT_2_PENALTY=0.753052
PRIMER_RIGHT_2_PENALTY=0.888344
PRIMER_LEFT_2_SEQUENCE=TATCCGTGATTGGGTTGTTG
PRIMER_RIGHT_2_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_2=713,20
PRIMER_RIGHT_2=1644,20
PRIMER_LEFT_2_TM=59.247
PRIMER_RIGHT_2_TM=59.112
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=932
PRIMER_PAIR_3_PENALTY=1.859947
PRIMER_LEFT_3_PENALTY=0.971603
PRIMER_RIGHT_3_PENALTY=0.888344
PRIMER_LEFT_3_SEQUENCE=CCATATCCGTGATTGGGTTG
PRIMER_RIGHT_3_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_3=710,20
PRIMER_RIGHT_3=1644,20
PRIMER_LEFT_3_TM=60.972
PRIMER_RIGHT_3_TM=59.112
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=935
PRIMER_PAIR_4_PENALTY=2.084342
PRIMER_LEFT_4_PENALTY=1.195998
PRIMER_RIGHT_4_PENALTY=0.888344
PRIMER_LEFT_4_SEQUENCE=CTGCCGTAATGGAGAGTGGA
PRIMER_RIGHT_4_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_4=912,20
PRIMER_RIGHT_4=1644,20
PRIMER_LEFT_4_TM=61.196
PRIMER_RIGHT_4_TM=59.112
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=733
PRIMER_PAIR_5_PENALTY=2.328281
PRIMER_LEFT_5_PENALTY=1.439937
PRIMER_RIGHT_5_PENALTY=0.888344
PRIMER_LEFT_5_SEQUENCE=ATATCCGTGATTGGGTTGTTG
PRIMER_RIGHT_5_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_5=712,21
PRIMER_RIGHT_5=1644,20
PRIMER_LEFT_5_TM=59.560
PRIMER_RIGHT_5_TM=59.112
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=10.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=933
PRIMER_PAIR_6_PENALTY=2.328281
PRIMER_LEFT_6_PENALTY=1.439937
PRIMER_RIGHT_6_PENALTY=0.888344
PRIMER_LEFT_6_SEQUENCE=CATATCCGTGATTGGGTTGTT
PRIMER_RIGHT_6_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_6=711,21
PRIMER_RIGHT_6=1644,20
PRIMER_LEFT_6_TM=59.560
PRIMER_RIGHT_6_TM=59.112
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=10.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=934
PRIMER_PAIR_7_PENALTY=2.687498
PRIMER_LEFT_7_PENALTY=1.799154
PRIMER_RIGHT_7_PENALTY=0.888344
PRIMER_LEFT_7_SEQUENCE=TTCTCAGGGCACATTTGTTT
PRIMER_RIGHT_7_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_7=825,20
PRIMER_RIGHT_7=1644,20
PRIMER_LEFT_7_TM=58.201
PRIMER_RIGHT_7_TM=59.112
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=10.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=820
PRIMER_PAIR_8_PENALTY=2.743237
PRIMER_LEFT_8_PENALTY=1.854893
PRIMER_RIGHT_8_PENALTY=0.888344
PRIMER_LEFT_8_SEQUENCE=TTGATTTCTCAGGGCACATT
PRIMER_RIGHT_8_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_8=820,20
PRIMER_RIGHT_8=1644,20
PRIMER_LEFT_8_TM=58.145
PRIMER_RIGHT_8_TM=59.112
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=10.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=825
PRIMER_PAIR_9_PENALTY=2.743237
PRIMER_LEFT_9_PENALTY=1.854893
PRIMER_RIGHT_9_PENALTY=0.888344
PRIMER_LEFT_9_SEQUENCE=CATATCCGTGATTGGGTTGT
PRIMER_RIGHT_9_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_9=711,20
PRIMER_RIGHT_9=1644,20
PRIMER_LEFT_9_TM=58.145
PRIMER_RIGHT_9_TM=59.112
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=10.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=934
PRIMER_PAIR_10_PENALTY=3.484204
PRIMER_LEFT_10_PENALTY=2.595860
PRIMER_RIGHT_10_PENALTY=0.888344
PRIMER_LEFT_10_SEQUENCE=TCTCAGGGCACATTTGTTTCA
PRIMER_RIGHT_10_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_10=826,21
PRIMER_RIGHT_10=1644,20
PRIMER_LEFT_10_TM=61.596
PRIMER_RIGHT_10_TM=59.112
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=10.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=819
PRIMER_PAIR_11_PENALTY=3.631971
PRIMER_LEFT_11_PENALTY=2.743627
PRIMER_RIGHT_11_PENALTY=0.888344
PRIMER_LEFT_11_SEQUENCE=CCATATCCGTGATTGGGTTGT
PRIMER_RIGHT_11_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_11=710,21
PRIMER_RIGHT_11=1644,20
PRIMER_LEFT_11_TM=61.744
PRIMER_RIGHT_11_TM=59.112
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=10.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=935
PRIMER_PAIR_12_PENALTY=3.631971
PRIMER_LEFT_12_PENALTY=2.743627
PRIMER_RIGHT_12_PENALTY=0.888344
PRIMER_LEFT_12_SEQUENCE=ACCATATCCGTGATTGGGTTG
PRIMER_RIGHT_12_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_12=709,21
PRIMER_RIGHT_12=1644,20
PRIMER_LEFT_12_TM=61.744
PRIMER_RIGHT_12_TM=59.112
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=10.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=936
PRIMER_PAIR_13_PENALTY=3.765439
PRIMER_LEFT_13_PENALTY=2.877095
PRIMER_RIGHT_13_PENALTY=0.888344
PRIMER_LEFT_13_SEQUENCE=ATCCGTGATTGGGTTGTTGG
PRIMER_RIGHT_13_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_13=714,20
PRIMER_RIGHT_13=1644,20
PRIMER_LEFT_13_TM=62.877
PRIMER_RIGHT_13_TM=59.112
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=10.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=931
PRIMER_PAIR_14_PENALTY=3.777534
PRIMER_LEFT_14_PENALTY=2.889190
PRIMER_RIGHT_14_PENALTY=0.888344
PRIMER_LEFT_14_SEQUENCE=CCTGCCGTAATGGAGAGTGG
PRIMER_RIGHT_14_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_14=911,20
PRIMER_RIGHT_14=1644,20
PRIMER_LEFT_14_TM=62.889
PRIMER_RIGHT_14_TM=59.112
PRIMER_LEFT_14_GC_PERCENT=60.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=10.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=734
PRIMER_PAIR_15_PENALTY=4.169214
PRIMER_LEFT_15_PENALTY=3.280870
PRIMER_RIGHT_15_PENALTY=0.888344
PRIMER_LEFT_15_SEQUENCE=ATATCCGTGATTGGGTTGTT
PRIMER_RIGHT_15_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_15=712,20
PRIMER_RIGHT_15=1644,20
PRIMER_LEFT_15_TM=56.719
PRIMER_RIGHT_15_TM=59.112
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=10.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=933
PRIMER_PAIR_16_PENALTY=4.239543
PRIMER_LEFT_16_PENALTY=3.351199
PRIMER_RIGHT_16_PENALTY=0.888344
PRIMER_LEFT_16_SEQUENCE=GCTGGACTTATCTACTCCTTCCC
PRIMER_RIGHT_16_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_16=883,23
PRIMER_RIGHT_16=1644,20
PRIMER_LEFT_16_TM=59.649
PRIMER_RIGHT_16_TM=59.112
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=10.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=762
PRIMER_PAIR_17_PENALTY=4.287947
PRIMER_LEFT_17_PENALTY=3.399603
PRIMER_RIGHT_17_PENALTY=0.888344
PRIMER_LEFT_17_SEQUENCE=TGGACTTATCTACTCCTTCCCC
PRIMER_RIGHT_17_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_17=885,22
PRIMER_RIGHT_17=1644,20
PRIMER_LEFT_17_TM=58.600
PRIMER_RIGHT_17_TM=59.112
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=10.9000
PRIMER_RIGHT_17_END_STABILITY=10.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=760
PRIMER_PAIR_18_PENALTY=4.370988
PRIMER_LEFT_18_PENALTY=3.482644
PRIMER_RIGHT_18_PENALTY=0.888344
PRIMER_LEFT_18_SEQUENCE=CTGGACTTATCTACTCCTTCCCC
PRIMER_RIGHT_18_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_18=884,23
PRIMER_RIGHT_18=1644,20
PRIMER_LEFT_18_TM=59.517
PRIMER_RIGHT_18_TM=59.112
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=10.9000
PRIMER_RIGHT_18_END_STABILITY=10.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=761
PRIMER_PAIR_19_PENALTY=4.667640
PRIMER_LEFT_19_PENALTY=3.779296
PRIMER_RIGHT_19_PENALTY=0.888344
PRIMER_LEFT_19_SEQUENCE=TATCCGTGATTGGGTTGTTGG
PRIMER_RIGHT_19_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_19=713,21
PRIMER_RIGHT_19=1644,20
PRIMER_LEFT_19_TM=62.779
PRIMER_RIGHT_19_TM=59.112
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=10.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=932
PRIMER_PAIR_20_PENALTY=4.891128
PRIMER_LEFT_20_PENALTY=4.002784
PRIMER_RIGHT_20_PENALTY=0.888344
PRIMER_LEFT_20_SEQUENCE=GCCATTATCAAGGCCAGGAAG
PRIMER_RIGHT_20_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_20=651,21
PRIMER_RIGHT_20=1644,20
PRIMER_LEFT_20_TM=63.003
PRIMER_RIGHT_20_TM=59.112
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=10.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=994
PRIMER_PAIR_21_PENALTY=4.920718
PRIMER_LEFT_21_PENALTY=4.032374
PRIMER_RIGHT_21_PENALTY=0.888344
PRIMER_LEFT_21_SEQUENCE=GGACTTATCTACTCCTTCCCCGT
PRIMER_RIGHT_21_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_21=886,23
PRIMER_RIGHT_21=1644,20
PRIMER_LEFT_21_TM=61.032
PRIMER_RIGHT_21_TM=59.112
PRIMER_LEFT_21_GC_PERCENT=52.174
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=11.1000
PRIMER_RIGHT_21_END_STABILITY=10.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=759
PRIMER_PAIR_22_PENALTY=5.071413
PRIMER_LEFT_22_PENALTY=4.183069
PRIMER_RIGHT_22_PENALTY=0.888344
PRIMER_LEFT_22_SEQUENCE=CAGCTGGACTTATCTACTCCTTCC
PRIMER_RIGHT_22_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_22=881,24
PRIMER_RIGHT_22=1644,20
PRIMER_LEFT_22_TM=59.817
PRIMER_RIGHT_22_TM=59.112
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=10.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=764
PRIMER_PAIR_23_PENALTY=5.083701
PRIMER_LEFT_23_PENALTY=4.195357
PRIMER_RIGHT_23_PENALTY=0.888344
PRIMER_LEFT_23_SEQUENCE=GACTTATCTACTCCTTCCCCGT
PRIMER_RIGHT_23_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_23=887,22
PRIMER_RIGHT_23=1644,20
PRIMER_LEFT_23_TM=57.805
PRIMER_RIGHT_23_TM=59.112
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=11.1000
PRIMER_RIGHT_23_END_STABILITY=10.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=758
PRIMER_PAIR_24_PENALTY=5.129499
PRIMER_LEFT_24_PENALTY=4.241155
PRIMER_RIGHT_24_PENALTY=0.888344
PRIMER_LEFT_24_SEQUENCE=CATATCCGTGATTGGGTTGTTG
PRIMER_RIGHT_24_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_24=711,22
PRIMER_RIGHT_24=1644,20
PRIMER_LEFT_24_TM=62.241
PRIMER_RIGHT_24_TM=59.112
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=10.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=934
PRIMER_PAIR_25_PENALTY=5.346619
PRIMER_LEFT_25_PENALTY=4.458275
PRIMER_RIGHT_25_PENALTY=0.888344
PRIMER_LEFT_25_SEQUENCE=ACCATATCCGTGATTGGGTTGT
PRIMER_RIGHT_25_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_25=709,22
PRIMER_RIGHT_25=1644,20
PRIMER_LEFT_25_TM=62.458
PRIMER_RIGHT_25_TM=59.112
PRIMER_LEFT_25_GC_PERCENT=45.455
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=10.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=936
PRIMER_PAIR_26_PENALTY=5.374091
PRIMER_LEFT_26_PENALTY=4.485747
PRIMER_RIGHT_26_PENALTY=0.888344
PRIMER_LEFT_26_SEQUENCE=AGCTGGACTTATCTACTCCTTCCC
PRIMER_RIGHT_26_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_26=882,24
PRIMER_RIGHT_26=1644,20
PRIMER_LEFT_26_TM=60.486
PRIMER_RIGHT_26_TM=59.112
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=10.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=763
PRIMER_PAIR_27_PENALTY=5.473732
PRIMER_LEFT_27_PENALTY=4.585388
PRIMER_RIGHT_27_PENALTY=0.888344
PRIMER_LEFT_27_SEQUENCE=TCCGTGATTGGGTTGTTGGA
PRIMER_RIGHT_27_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_27=715,20
PRIMER_RIGHT_27=1644,20
PRIMER_LEFT_27_TM=64.585
PRIMER_RIGHT_27_TM=59.112
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=10.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=930
PRIMER_PAIR_28_PENALTY=5.696193
PRIMER_LEFT_28_PENALTY=4.807850
PRIMER_RIGHT_28_PENALTY=0.888344
PRIMER_LEFT_28_SEQUENCE=TTCTCAGGGCACATTTGTTTCA
PRIMER_RIGHT_28_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_28=825,22
PRIMER_RIGHT_28=1644,20
PRIMER_LEFT_28_TM=62.808
PRIMER_RIGHT_28_TM=59.112
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=10.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=820
PRIMER_PAIR_29_PENALTY=5.784354
PRIMER_LEFT_29_PENALTY=4.896010
PRIMER_RIGHT_29_PENALTY=0.888344
PRIMER_LEFT_29_SEQUENCE=ATATCCGTGATTGGGTTGTTGG
PRIMER_RIGHT_29_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_29=712,22
PRIMER_RIGHT_29=1644,20
PRIMER_LEFT_29_TM=62.896
PRIMER_RIGHT_29_TM=59.112
PRIMER_LEFT_29_GC_PERCENT=45.455
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=10.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=933
PRIMER_PAIR_30_PENALTY=5.784354
PRIMER_LEFT_30_PENALTY=4.896010
PRIMER_RIGHT_30_PENALTY=0.888344
PRIMER_LEFT_30_SEQUENCE=CCATATCCGTGATTGGGTTGTT
PRIMER_RIGHT_30_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_30=710,22
PRIMER_RIGHT_30=1644,20
PRIMER_LEFT_30_TM=62.896
PRIMER_RIGHT_30_TM=59.112
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=10.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=935
PRIMER_PAIR_31_PENALTY=5.836112
PRIMER_LEFT_31_PENALTY=4.947768
PRIMER_RIGHT_31_PENALTY=0.888344
PRIMER_LEFT_31_SEQUENCE=GGACCATTGTTCAAGGTAAA
PRIMER_RIGHT_31_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_31=929,20
PRIMER_RIGHT_31=1644,20
PRIMER_LEFT_31_TM=55.052
PRIMER_RIGHT_31_TM=59.112
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.0000
PRIMER_RIGHT_31_END_STABILITY=10.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=716
PRIMER_PAIR_32_PENALTY=5.872385
PRIMER_LEFT_32_PENALTY=4.984041
PRIMER_RIGHT_32_PENALTY=0.888344
PRIMER_LEFT_32_SEQUENCE=CTTATCTACTCCTTCCCCGT
PRIMER_RIGHT_32_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_32=889,20
PRIMER_RIGHT_32=1644,20
PRIMER_LEFT_32_TM=55.016
PRIMER_RIGHT_32_TM=59.112
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=11.1000
PRIMER_RIGHT_32_END_STABILITY=10.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=756
PRIMER_PAIR_33_PENALTY=5.901273
PRIMER_LEFT_33_PENALTY=5.012929
PRIMER_RIGHT_33_PENALTY=0.888344
PRIMER_LEFT_33_SEQUENCE=ACTTATCTACTCCTTCCCCGT
PRIMER_RIGHT_33_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_33=888,21
PRIMER_RIGHT_33=1644,20
PRIMER_LEFT_33_TM=55.987
PRIMER_RIGHT_33_TM=59.112
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=11.1000
PRIMER_RIGHT_33_END_STABILITY=10.2000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=757
PRIMER_PAIR_34_PENALTY=6.002548
PRIMER_LEFT_34_PENALTY=5.114204
PRIMER_RIGHT_34_PENALTY=0.888344
PRIMER_LEFT_34_SEQUENCE=GGACTTATCTACTCCTTCCCC
PRIMER_RIGHT_34_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_34=886,21
PRIMER_RIGHT_34=1644,20
PRIMER_LEFT_34_TM=55.886
PRIMER_RIGHT_34_TM=59.112
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=10.9000
PRIMER_RIGHT_34_END_STABILITY=10.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=759
PRIMER_PAIR_35_PENALTY=6.207481
PRIMER_LEFT_35_PENALTY=5.319137
PRIMER_RIGHT_35_PENALTY=0.888344
PRIMER_LEFT_35_SEQUENCE=CTCAGATGGATCCTACAATGTTC
PRIMER_RIGHT_35_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_35=858,23
PRIMER_RIGHT_35=1644,20
PRIMER_LEFT_35_TM=57.681
PRIMER_RIGHT_35_TM=59.112
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=10.2000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=787
PRIMER_PAIR_36_PENALTY=6.294136
PRIMER_LEFT_36_PENALTY=5.405792
PRIMER_RIGHT_36_PENALTY=0.888344
PRIMER_LEFT_36_SEQUENCE=TCAGATGGATCCTACAATGTTC
PRIMER_RIGHT_36_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_36=859,22
PRIMER_RIGHT_36=1644,20
PRIMER_LEFT_36_TM=56.594
PRIMER_RIGHT_36_TM=59.112
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=10.2000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=786
PRIMER_PAIR_37_PENALTY=6.350331
PRIMER_LEFT_37_PENALTY=5.461987
PRIMER_RIGHT_37_PENALTY=0.888344
PRIMER_LEFT_37_SEQUENCE=ACTCAGATGGATCCTACAATGTTC
PRIMER_RIGHT_37_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_37=857,24
PRIMER_RIGHT_37=1644,20
PRIMER_LEFT_37_TM=58.538
PRIMER_RIGHT_37_TM=59.112
PRIMER_LEFT_37_GC_PERCENT=41.667
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=10.2000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=788
PRIMER_PAIR_38_PENALTY=6.497252
PRIMER_LEFT_38_PENALTY=5.608908
PRIMER_RIGHT_38_PENALTY=0.888344
PRIMER_LEFT_38_SEQUENCE=CCTGCCGTAATGGAGAGTGGA
PRIMER_RIGHT_38_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_38=911,21
PRIMER_RIGHT_38=1644,20
PRIMER_LEFT_38_TM=64.609
PRIMER_RIGHT_38_TM=59.112
PRIMER_LEFT_38_GC_PERCENT=57.143
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=10.2000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=734
PRIMER_PAIR_39_PENALTY=6.500597
PRIMER_LEFT_39_PENALTY=5.612253
PRIMER_RIGHT_39_PENALTY=0.888344
PRIMER_LEFT_39_SEQUENCE=ATCCGTGATTGGGTTGTTGGA
PRIMER_RIGHT_39_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_39=714,21
PRIMER_RIGHT_39=1644,20
PRIMER_LEFT_39_TM=64.612
PRIMER_RIGHT_39_TM=59.112
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=10.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=931
PRIMER_PAIR_40_PENALTY=6.597116
PRIMER_LEFT_40_PENALTY=5.708772
PRIMER_RIGHT_40_PENALTY=0.888344
PRIMER_LEFT_40_SEQUENCE=AGCTGGACTTATCTACTCCTTCC
PRIMER_RIGHT_40_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_40=882,23
PRIMER_RIGHT_40=1644,20
PRIMER_LEFT_40_TM=57.291
PRIMER_RIGHT_40_TM=59.112
PRIMER_LEFT_40_GC_PERCENT=47.826
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=10.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=763
PRIMER_PAIR_41_PENALTY=6.624973
PRIMER_LEFT_41_PENALTY=5.736629
PRIMER_RIGHT_41_PENALTY=0.888344
PRIMER_LEFT_41_SEQUENCE=GCTGGACTTATCTACTCCTTCC
PRIMER_RIGHT_41_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_41=883,22
PRIMER_RIGHT_41=1644,20
PRIMER_LEFT_41_TM=56.263
PRIMER_RIGHT_41_TM=59.112
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=10.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=762
PRIMER_PAIR_42_PENALTY=6.764674
PRIMER_LEFT_42_PENALTY=5.876330
PRIMER_RIGHT_42_PENALTY=0.888344
PRIMER_LEFT_42_SEQUENCE=CTGGACTTATCTACTCCTTCCC
PRIMER_RIGHT_42_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_42=884,22
PRIMER_RIGHT_42=1644,20
PRIMER_LEFT_42_TM=56.124
PRIMER_RIGHT_42_TM=59.112
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=10.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=761
PRIMER_PAIR_43_PENALTY=7.289801
PRIMER_LEFT_43_PENALTY=6.401457
PRIMER_RIGHT_43_PENALTY=0.888344
PRIMER_LEFT_43_SEQUENCE=TACTCAGATGGATCCTACAATGTTC
PRIMER_RIGHT_43_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_43=856,25
PRIMER_RIGHT_43=1644,20
PRIMER_LEFT_43_TM=58.599
PRIMER_RIGHT_43_TM=59.112
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=10.2000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=789
PRIMER_PAIR_44_PENALTY=7.342165
PRIMER_LEFT_44_PENALTY=6.453821
PRIMER_RIGHT_44_PENALTY=0.888344
PRIMER_LEFT_44_SEQUENCE=TATCCGTGATTGGGTTGTTGGA
PRIMER_RIGHT_44_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_44=713,22
PRIMER_RIGHT_44=1644,20
PRIMER_LEFT_44_TM=64.454
PRIMER_RIGHT_44_TM=59.112
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=10.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=932
PRIMER_PAIR_45_PENALTY=7.396412
PRIMER_LEFT_45_PENALTY=6.508068
PRIMER_RIGHT_45_PENALTY=0.888344
PRIMER_LEFT_45_SEQUENCE=CAGCTGGACTTATCTACTCCTTC
PRIMER_RIGHT_45_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_45=881,23
PRIMER_RIGHT_45=1644,20
PRIMER_LEFT_45_TM=56.492
PRIMER_RIGHT_45_TM=59.112
PRIMER_LEFT_45_GC_PERCENT=47.826
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=10.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=764
PRIMER_PAIR_46_PENALTY=7.423244
PRIMER_LEFT_46_PENALTY=6.534900
PRIMER_RIGHT_46_PENALTY=0.888344
PRIMER_LEFT_46_SEQUENCE=ACCATATCCGTGATTGGGTTGTT
PRIMER_RIGHT_46_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_46=709,23
PRIMER_RIGHT_46=1644,20
PRIMER_LEFT_46_TM=63.535
PRIMER_RIGHT_46_TM=59.112
PRIMER_LEFT_46_GC_PERCENT=43.478
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=10.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=936
PRIMER_PAIR_47_PENALTY=7.574788
PRIMER_LEFT_47_PENALTY=6.686444
PRIMER_RIGHT_47_PENALTY=0.888344
PRIMER_LEFT_47_SEQUENCE=GCTGGACTTATCTACTCCTTCCCC
PRIMER_RIGHT_47_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_47=883,24
PRIMER_RIGHT_47=1644,20
PRIMER_LEFT_47_TM=62.686
PRIMER_RIGHT_47_TM=59.112
PRIMER_LEFT_47_GC_PERCENT=54.167
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=10.9000
PRIMER_RIGHT_47_END_STABILITY=10.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=762
PRIMER_PAIR_48_PENALTY=7.970069
PRIMER_LEFT_48_PENALTY=7.081725
PRIMER_RIGHT_48_PENALTY=0.888344
PRIMER_LEFT_48_SEQUENCE=GACCATATCCGTGATTGGGTTGT
PRIMER_RIGHT_48_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_48=708,23
PRIMER_RIGHT_48=1644,20
PRIMER_LEFT_48_TM=64.082
PRIMER_RIGHT_48_TM=59.112
PRIMER_LEFT_48_GC_PERCENT=47.826
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=10.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=937
PRIMER_PAIR_49_PENALTY=8.269005
PRIMER_LEFT_49_PENALTY=7.380662
PRIMER_RIGHT_49_PENALTY=0.888344
PRIMER_LEFT_49_SEQUENCE=TGGACTTATCTACTCCTTCCCCGT
PRIMER_RIGHT_49_SEQUENCE=TCCCATTGTTCTAAATCGCA
PRIMER_LEFT_49=885,24
PRIMER_RIGHT_49=1644,20
PRIMER_LEFT_49_TM=63.381
PRIMER_RIGHT_49_TM=59.112
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=11.1000
PRIMER_RIGHT_49_END_STABILITY=10.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=760
=
SEQUENCE_ID=MAGIC.105_4_17775027_17775260_edi-0_oy-0_8532_1694_TRUE
SEQUENCE_TARGET=950,333
SEQUENCE_TEMPLATE=ANNAAAAAAACTTGCAAANTTTNAATGAAAGATTGCATGCATTTAAGTGATCGTTNNNNNNNNNAAACACGCGGGTCTTATGAGTTTCATTTTCCATCGCATCACATGCTCTTCTAATCATGGTCAAAGCATTTTTCCGTTAGTCATGAAATTGACACGTGGAAAATACTNTTTTCGGAACACGTGGATGTTCCTCNANTGATCACATCNTTNTAATAGAATACTCTTTTCAGATACATACATAGTTAGAAGATACGTCTAAATTTNAACTANCGAAACTTAAAGAGTACAGAACATTATATATGATTAACATTTGTCTCGAGTCTCACGGCTTTAAAGATACAGTATTGAGCNGGTNTTNAATCANNNTACATCATCACTTTTCATTTCNTTATCGCTATNNNNNNNNNNNNNNNNNNNNNNATTTAATAAAAGATGGAAACAGCCAAACAACAGGTCAATATGGAANTTTCAAGTTGTATTTAAAAGAAAAGAAAAAGCCNTTAGCTTAGTCATCACTCATCAATTCGTTCCCAGAAAGTTACAAATTTGGACCATTCCAATTATCTCTAATGGACCCCAGTAATCTTATCTTTTCGCTANTTTATCCCATAACTTTACTTTTTTAATCAAATCAAACTCTGTTTTGTAGCCATCTCATGTGCTGGCCTCATCCGAGAAATTAAACCCCATTNAATTACCATCATCACAATCATCATCTTTTGCCATCTGCNTCGTGGGCTTTGTTTTTCTTGCCATCAATATCAACAAAGGACTAAGACACTTTTTACCAACTTTACGTACGGACTATTCCATATTTGATCTTTAACCTTTTAGAATTCAGATTTTGGTTTTGTAANTTTACGGACCAGGCTATGGTTAACTACAAAAAGTAAANTAAAAATAATGGCCTCATGGGCTTGNCGGNCTAACCTAATTTGANTGGCTCTCGCATGGTTGATCATCAATGGATCAGACCGAAATCTAAAGCCTAATGTTANTTTTCTTTGGTTTAAAACCCTNATTTTTTCAAAACCTAAATTGCGCACAAGACTATGTAAATTGGATCGACCGGACTATATGAAAACCTCAAAGCATATTATTGCCTCAAAGCCCAAGACAATACTGTTAAAGAGAAGAAGGNAAAGGTCACCAGGCCCATTAAGATGAAAGTAATTTTCAGACCCAGGGGACCAAACAACCCCAATCATGAAGACCAAATTTCTGTTTTAGNGGAAAAGAGNANGGAATATTAAATTCAACTGNANAAAACAATTATAANACATGGTCNNTAACCAAGAAATATACTGCAGGNTTTGTTATAATCAATAGTCTCATTCTCAATACTGATGNATTTTAGCTTAATATTGATTTCTTTTTCTCTTGTGTTAGATTTAATATTCCATGTGAAACTGTTCCATACCNANCGAGGGCCAAAATACCCACTCCAACAAACAAACATGGTTCTCTTCAATAGATCAACAAAACATAAGACATCATGAAAACAAAGTAAAATTCAAAGATATANATATATATATGAACATTTGACCACAATCANATCGATTATGAGCAATTCACCACATACCACAAGACTCATTAATCNCTTCANATACATTGNAACNTTGCTTANGGAACCACTTNGCACTTGTNTTGCTTTNAAGACAATAATGNTTGGATCANATTCCNGCCGAGCATTCTGGAGGTGGAACAGGAAAACGAGATTTGTCNGTGCAATAATCGTAGACCATATGGTTCACTCGGACCCATCTATAACTTCGAGCTTCCACCGGACTCAACTGGTGGTACGCACTGCCTTCCCACCAATTNTTCGAATTAGCGGGACAATNTGNGGGTCCTGGAACCGGACATCCTTCTATATCAAAATCTTTGTAATAAGCATAAAATGGCGCTCTCGACCAATTGATTTTCTCTATTCCTCCACGTGTCGCCCAATCATCGGCTTCCCATAACGTGGAATATACACCCATTGGTTGGAATCTTGGGTATGGTACTTTCCTTGCCTCATTGTTCTTGNAAACCCTGATGGGCACATTGTCTACGTAGAAGCTACACACCACACATATTTAGACTATTAGAAAGTGAATAACAGTTCTACATATATTGTTANTGCAAATAGAAATATCTTTGATCATTNNTTTTTTTNTTCTTATAAACTNATGGCNNNTGCCTAATNGATTATNAAAATGTCATTACNAAAAAAAATGGNTTATNA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.332685
PRIMER_LEFT_0_PENALTY=0.275749
PRIMER_RIGHT_0_PENALTY=0.056936
PRIMER_LEFT_0_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_0_SEQUENCE=GTTTTCCTGTTCCACCTCCA
PRIMER_LEFT_0=734,20
PRIMER_RIGHT_0=1716,20
PRIMER_LEFT_0_TM=59.724
PRIMER_RIGHT_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=983
PRIMER_PAIR_1_PENALTY=0.364045
PRIMER_LEFT_1_PENALTY=0.275749
PRIMER_RIGHT_1_PENALTY=0.088296
PRIMER_LEFT_1_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_1_SEQUENCE=TCGTTTTCCTGTTCCACCTC
PRIMER_LEFT_1=734,20
PRIMER_RIGHT_1=1718,20
PRIMER_LEFT_1_TM=59.724
PRIMER_RIGHT_1_TM=60.088
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=985
PRIMER_PAIR_2_PENALTY=0.364045
PRIMER_LEFT_2_PENALTY=0.275749
PRIMER_RIGHT_2_PENALTY=0.088296
PRIMER_LEFT_2_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_2_SEQUENCE=TCTCGTTTTCCTGTTCCACC
PRIMER_LEFT_2=734,20
PRIMER_RIGHT_2=1720,20
PRIMER_LEFT_2_TM=59.724
PRIMER_RIGHT_2_TM=60.088
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=987
PRIMER_PAIR_3_PENALTY=0.696108
PRIMER_LEFT_3_PENALTY=0.639172
PRIMER_RIGHT_3_PENALTY=0.056936
PRIMER_LEFT_3_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_3_SEQUENCE=GTTTTCCTGTTCCACCTCCA
PRIMER_LEFT_3=735,20
PRIMER_RIGHT_3=1716,20
PRIMER_LEFT_3_TM=60.639
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=982
PRIMER_PAIR_4_PENALTY=0.727468
PRIMER_LEFT_4_PENALTY=0.639172
PRIMER_RIGHT_4_PENALTY=0.088296
PRIMER_LEFT_4_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_4_SEQUENCE=TCGTTTTCCTGTTCCACCTC
PRIMER_LEFT_4=735,20
PRIMER_RIGHT_4=1718,20
PRIMER_LEFT_4_TM=60.639
PRIMER_RIGHT_4_TM=60.088
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=984
PRIMER_PAIR_5_PENALTY=0.727468
PRIMER_LEFT_5_PENALTY=0.639172
PRIMER_RIGHT_5_PENALTY=0.088296
PRIMER_LEFT_5_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_5_SEQUENCE=TCTCGTTTTCCTGTTCCACC
PRIMER_LEFT_5=735,20
PRIMER_RIGHT_5=1720,20
PRIMER_LEFT_5_TM=60.639
PRIMER_RIGHT_5_TM=60.088
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=986
PRIMER_PAIR_6_PENALTY=0.776577
PRIMER_LEFT_6_PENALTY=0.275749
PRIMER_RIGHT_6_PENALTY=0.500828
PRIMER_LEFT_6_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_6_SEQUENCE=CCTGTTCCACCTCCAGAATG
PRIMER_LEFT_6=734,20
PRIMER_RIGHT_6=1711,20
PRIMER_LEFT_6_TM=59.724
PRIMER_RIGHT_6_TM=60.501
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=978
PRIMER_PAIR_7_PENALTY=0.840840
PRIMER_LEFT_7_PENALTY=0.275749
PRIMER_RIGHT_7_PENALTY=0.565091
PRIMER_LEFT_7_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_7_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_7=734,20
PRIMER_RIGHT_7=1460,20
PRIMER_LEFT_7_TM=59.724
PRIMER_RIGHT_7_TM=59.435
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=1.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=727
PRIMER_PAIR_8_PENALTY=0.900238
PRIMER_LEFT_8_PENALTY=0.275749
PRIMER_RIGHT_8_PENALTY=0.624489
PRIMER_LEFT_8_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_8_SEQUENCE=TTCCTGTTCCACCTCCAGAA
PRIMER_LEFT_8=734,20
PRIMER_RIGHT_8=1713,20
PRIMER_LEFT_8_TM=59.724
PRIMER_RIGHT_8_TM=60.624
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=980
PRIMER_PAIR_9_PENALTY=0.968447
PRIMER_LEFT_9_PENALTY=0.275749
PRIMER_RIGHT_9_PENALTY=0.692698
PRIMER_LEFT_9_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_9_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_9=734,20
PRIMER_RIGHT_9=1447,20
PRIMER_LEFT_9_TM=59.724
PRIMER_RIGHT_9_TM=60.693
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=714
PRIMER_PAIR_10_PENALTY=1.092685
PRIMER_LEFT_10_PENALTY=0.275749
PRIMER_RIGHT_10_PENALTY=0.816936
PRIMER_LEFT_10_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_10_SEQUENCE=CTCGTTTTCCTGTTCCACCT
PRIMER_LEFT_10=734,20
PRIMER_RIGHT_10=1719,20
PRIMER_LEFT_10_TM=59.724
PRIMER_RIGHT_10_TM=59.183
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=986
PRIMER_PAIR_11_PENALTY=1.140000
PRIMER_LEFT_11_PENALTY=0.639172
PRIMER_RIGHT_11_PENALTY=0.500828
PRIMER_LEFT_11_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_11_SEQUENCE=CCTGTTCCACCTCCAGAATG
PRIMER_LEFT_11=735,20
PRIMER_RIGHT_11=1711,20
PRIMER_LEFT_11_TM=60.639
PRIMER_RIGHT_11_TM=60.501
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=977
PRIMER_PAIR_12_PENALTY=1.196403
PRIMER_LEFT_12_PENALTY=0.503705
PRIMER_RIGHT_12_PENALTY=0.692698
PRIMER_LEFT_12_SEQUENCE=CACTCATCAATTCGTTCCCA
PRIMER_RIGHT_12_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_12=516,20
PRIMER_RIGHT_12=1447,20
PRIMER_LEFT_12_TM=59.496
PRIMER_RIGHT_12_TM=60.693
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=932
PRIMER_PAIR_13_PENALTY=1.204263
PRIMER_LEFT_13_PENALTY=0.639172
PRIMER_RIGHT_13_PENALTY=0.565091
PRIMER_LEFT_13_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_13_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_13=735,20
PRIMER_RIGHT_13=1460,20
PRIMER_LEFT_13_TM=60.639
PRIMER_RIGHT_13_TM=59.435
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=1.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=726
PRIMER_PAIR_14_PENALTY=1.331870
PRIMER_LEFT_14_PENALTY=0.639172
PRIMER_RIGHT_14_PENALTY=0.692698
PRIMER_LEFT_14_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_14_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_14=735,20
PRIMER_RIGHT_14=1447,20
PRIMER_LEFT_14_TM=60.639
PRIMER_RIGHT_14_TM=60.693
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=9.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=713
PRIMER_PAIR_15_PENALTY=1.456108
PRIMER_LEFT_15_PENALTY=0.639172
PRIMER_RIGHT_15_PENALTY=0.816936
PRIMER_LEFT_15_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_15_SEQUENCE=CTCGTTTTCCTGTTCCACCT
PRIMER_LEFT_15=735,20
PRIMER_RIGHT_15=1719,20
PRIMER_LEFT_15_TM=60.639
PRIMER_RIGHT_15_TM=59.183
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=985
PRIMER_PAIR_16_PENALTY=1.532970
PRIMER_LEFT_16_PENALTY=0.275749
PRIMER_RIGHT_16_PENALTY=1.257221
PRIMER_LEFT_16_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_16_SEQUENCE=ATGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_16=734,20
PRIMER_RIGHT_16=1461,21
PRIMER_LEFT_16_TM=59.724
PRIMER_RIGHT_16_TM=59.743
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=2.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=728
PRIMER_PAIR_17_PENALTY=1.565529
PRIMER_LEFT_17_PENALTY=0.275749
PRIMER_RIGHT_17_PENALTY=1.289780
PRIMER_LEFT_17_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_17_SEQUENCE=TTGTTGGAGTGGGTATTTTGG
PRIMER_LEFT_17=734,20
PRIMER_RIGHT_17=1453,21
PRIMER_LEFT_17_TM=59.724
PRIMER_RIGHT_17_TM=59.710
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.8000
PRIMER_PAIR_17_COMPL_ANY=2.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=720
PRIMER_PAIR_18_PENALTY=1.573160
PRIMER_LEFT_18_PENALTY=0.275749
PRIMER_RIGHT_18_PENALTY=1.297410
PRIMER_LEFT_18_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_18_SEQUENCE=TGTTTGTTTGTTGGAGTGGGT
PRIMER_LEFT_18=734,20
PRIMER_RIGHT_18=1460,21
PRIMER_LEFT_18_TM=59.724
PRIMER_RIGHT_18_TM=60.297
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=1.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=2.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=727
PRIMER_PAIR_19_PENALTY=1.630783
PRIMER_LEFT_19_PENALTY=0.275749
PRIMER_RIGHT_19_PENALTY=1.355034
PRIMER_LEFT_19_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_19_SEQUENCE=ATCTCGTTTTCCTGTTCCACC
PRIMER_LEFT_19=734,20
PRIMER_RIGHT_19=1721,21
PRIMER_LEFT_19_TM=59.724
PRIMER_RIGHT_19_TM=60.355
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=988
PRIMER_PAIR_20_PENALTY=1.701675
PRIMER_LEFT_20_PENALTY=1.136583
PRIMER_RIGHT_20_PENALTY=0.565091
PRIMER_LEFT_20_SEQUENCE=TCATCCGAGAAATTAAACCCC
PRIMER_RIGHT_20_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_20=670,21
PRIMER_RIGHT_20=1460,20
PRIMER_LEFT_20_TM=60.137
PRIMER_RIGHT_20_TM=59.435
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=1.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=10.6000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=791
PRIMER_PAIR_21_PENALTY=1.793485
PRIMER_LEFT_21_PENALTY=0.503705
PRIMER_RIGHT_21_PENALTY=1.289780
PRIMER_LEFT_21_SEQUENCE=CACTCATCAATTCGTTCCCA
PRIMER_RIGHT_21_SEQUENCE=TTGTTGGAGTGGGTATTTTGG
PRIMER_LEFT_21=516,20
PRIMER_RIGHT_21=1453,21
PRIMER_LEFT_21_TM=59.496
PRIMER_RIGHT_21_TM=59.710
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=938
PRIMER_PAIR_22_PENALTY=1.801116
PRIMER_LEFT_22_PENALTY=0.503705
PRIMER_RIGHT_22_PENALTY=1.297410
PRIMER_LEFT_22_SEQUENCE=CACTCATCAATTCGTTCCCA
PRIMER_RIGHT_22_SEQUENCE=TGTTTGTTTGTTGGAGTGGGT
PRIMER_LEFT_22=516,20
PRIMER_RIGHT_22=1460,21
PRIMER_LEFT_22_TM=59.496
PRIMER_RIGHT_22_TM=60.297
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=1.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=945
PRIMER_PAIR_23_PENALTY=1.829281
PRIMER_LEFT_23_PENALTY=1.136583
PRIMER_RIGHT_23_PENALTY=0.692698
PRIMER_LEFT_23_SEQUENCE=TCATCCGAGAAATTAAACCCC
PRIMER_RIGHT_23_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_23=670,21
PRIMER_RIGHT_23=1447,20
PRIMER_LEFT_23_TM=60.137
PRIMER_RIGHT_23_TM=60.693
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=10.6000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=778
PRIMER_PAIR_24_PENALTY=1.896393
PRIMER_LEFT_24_PENALTY=0.639172
PRIMER_RIGHT_24_PENALTY=1.257221
PRIMER_LEFT_24_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_24_SEQUENCE=ATGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_24=735,20
PRIMER_RIGHT_24=1461,21
PRIMER_LEFT_24_TM=60.639
PRIMER_RIGHT_24_TM=59.743
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=727
PRIMER_PAIR_25_PENALTY=1.922353
PRIMER_LEFT_25_PENALTY=1.357262
PRIMER_RIGHT_25_PENALTY=0.565091
PRIMER_LEFT_25_SEQUENCE=CTCATCAATTCGTTCCCAGA
PRIMER_RIGHT_25_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_25=518,20
PRIMER_RIGHT_25=1460,20
PRIMER_LEFT_25_TM=58.643
PRIMER_RIGHT_25_TM=59.435
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=1.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=943
PRIMER_PAIR_26_PENALTY=1.928952
PRIMER_LEFT_26_PENALTY=0.639172
PRIMER_RIGHT_26_PENALTY=1.289780
PRIMER_LEFT_26_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_26_SEQUENCE=TTGTTGGAGTGGGTATTTTGG
PRIMER_LEFT_26=735,20
PRIMER_RIGHT_26=1453,21
PRIMER_LEFT_26_TM=60.639
PRIMER_RIGHT_26_TM=59.710
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=719
PRIMER_PAIR_27_PENALTY=1.936582
PRIMER_LEFT_27_PENALTY=0.639172
PRIMER_RIGHT_27_PENALTY=1.297410
PRIMER_LEFT_27_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_27_SEQUENCE=TGTTTGTTTGTTGGAGTGGGT
PRIMER_LEFT_27=735,20
PRIMER_RIGHT_27=1460,21
PRIMER_LEFT_27_TM=60.639
PRIMER_RIGHT_27_TM=60.297
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=1.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=726
PRIMER_PAIR_28_PENALTY=1.951234
PRIMER_LEFT_28_PENALTY=0.275749
PRIMER_RIGHT_28_PENALTY=1.675485
PRIMER_LEFT_28_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_28_SEQUENCE=TGTTGGAGTGGGTATTTTGG
PRIMER_LEFT_28=734,20
PRIMER_RIGHT_28=1452,20
PRIMER_LEFT_28_TM=59.724
PRIMER_RIGHT_28_TM=58.325
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=8.8000
PRIMER_PAIR_28_COMPL_ANY=2.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=719
PRIMER_PAIR_29_PENALTY=1.994205
PRIMER_LEFT_29_PENALTY=0.639172
PRIMER_RIGHT_29_PENALTY=1.355034
PRIMER_LEFT_29_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_29_SEQUENCE=ATCTCGTTTTCCTGTTCCACC
PRIMER_LEFT_29=735,20
PRIMER_RIGHT_29=1721,21
PRIMER_LEFT_29_TM=60.639
PRIMER_RIGHT_29_TM=60.355
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=9.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=987
PRIMER_PAIR_30_PENALTY=2.010241
PRIMER_LEFT_30_PENALTY=1.953305
PRIMER_RIGHT_30_PENALTY=0.056936
PRIMER_LEFT_30_SEQUENCE=GGCTTTGTTTTTCTTGCCATC
PRIMER_RIGHT_30_SEQUENCE=GTTTTCCTGTTCCACCTCCA
PRIMER_LEFT_30=739,21
PRIMER_RIGHT_30=1716,20
PRIMER_LEFT_30_TM=60.953
PRIMER_RIGHT_30_TM=59.943
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=2.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=978
PRIMER_PAIR_31_PENALTY=2.024182
PRIMER_LEFT_31_PENALTY=1.459091
PRIMER_RIGHT_31_PENALTY=0.565091
PRIMER_LEFT_31_SEQUENCE=ACTCATCAATTCGTTCCCAGA
PRIMER_RIGHT_31_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_31=517,21
PRIMER_RIGHT_31=1460,20
PRIMER_LEFT_31_TM=59.541
PRIMER_RIGHT_31_TM=59.435
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=1.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=944
PRIMER_PAIR_32_PENALTY=2.041601
PRIMER_LEFT_32_PENALTY=1.953305
PRIMER_RIGHT_32_PENALTY=0.088296
PRIMER_LEFT_32_SEQUENCE=GGCTTTGTTTTTCTTGCCATC
PRIMER_RIGHT_32_SEQUENCE=TCGTTTTCCTGTTCCACCTC
PRIMER_LEFT_32=739,21
PRIMER_RIGHT_32=1718,20
PRIMER_LEFT_32_TM=60.953
PRIMER_RIGHT_32_TM=60.088
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=2.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=980
PRIMER_PAIR_33_PENALTY=2.041601
PRIMER_LEFT_33_PENALTY=1.953305
PRIMER_RIGHT_33_PENALTY=0.088296
PRIMER_LEFT_33_SEQUENCE=GGCTTTGTTTTTCTTGCCATC
PRIMER_RIGHT_33_SEQUENCE=TCTCGTTTTCCTGTTCCACC
PRIMER_LEFT_33=739,21
PRIMER_RIGHT_33=1720,20
PRIMER_LEFT_33_TM=60.953
PRIMER_RIGHT_33_TM=60.088
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=2.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=982
PRIMER_PAIR_34_PENALTY=2.049960
PRIMER_LEFT_34_PENALTY=1.357262
PRIMER_RIGHT_34_PENALTY=0.692698
PRIMER_LEFT_34_SEQUENCE=CTCATCAATTCGTTCCCAGA
PRIMER_RIGHT_34_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_34=518,20
PRIMER_RIGHT_34=1447,20
PRIMER_LEFT_34_TM=58.643
PRIMER_RIGHT_34_TM=60.693
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=930
PRIMER_PAIR_35_PENALTY=2.066458
PRIMER_LEFT_35_PENALTY=1.501367
PRIMER_RIGHT_35_PENALTY=0.565091
PRIMER_LEFT_35_SEQUENCE=CACTCATCAATTCGTTCCCAG
PRIMER_RIGHT_35_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_35=516,21
PRIMER_RIGHT_35=1460,20
PRIMER_LEFT_35_TM=60.501
PRIMER_RIGHT_35_TM=59.435
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=1.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=945
PRIMER_PAIR_36_PENALTY=2.076584
PRIMER_LEFT_36_PENALTY=1.511492
PRIMER_RIGHT_36_PENALTY=0.565091
PRIMER_LEFT_36_SEQUENCE=TCACTCATCAATTCGTTCCC
PRIMER_RIGHT_36_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_36=515,20
PRIMER_RIGHT_36=1460,20
PRIMER_LEFT_36_TM=58.489
PRIMER_RIGHT_36_TM=59.435
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=1.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=946
PRIMER_PAIR_37_PENALTY=2.084246
PRIMER_LEFT_37_PENALTY=0.275749
PRIMER_RIGHT_37_PENALTY=1.808497
PRIMER_LEFT_37_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_37_SEQUENCE=CGTTTTCCTGTTCCACCTCC
PRIMER_LEFT_37=734,20
PRIMER_RIGHT_37=1717,20
PRIMER_LEFT_37_TM=59.724
PRIMER_RIGHT_37_TM=61.808
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=984
PRIMER_PAIR_38_PENALTY=2.151789
PRIMER_LEFT_38_PENALTY=1.459091
PRIMER_RIGHT_38_PENALTY=0.692698
PRIMER_LEFT_38_SEQUENCE=ACTCATCAATTCGTTCCCAGA
PRIMER_RIGHT_38_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_38=517,21
PRIMER_RIGHT_38=1447,20
PRIMER_LEFT_38_TM=59.541
PRIMER_RIGHT_38_TM=60.693
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=931
PRIMER_PAIR_39_PENALTY=2.179191
PRIMER_LEFT_39_PENALTY=0.503705
PRIMER_RIGHT_39_PENALTY=1.675485
PRIMER_LEFT_39_SEQUENCE=CACTCATCAATTCGTTCCCA
PRIMER_RIGHT_39_SEQUENCE=TGTTGGAGTGGGTATTTTGG
PRIMER_LEFT_39=516,20
PRIMER_RIGHT_39=1452,20
PRIMER_LEFT_39_TM=59.496
PRIMER_RIGHT_39_TM=58.325
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=8.8000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=937
PRIMER_PAIR_40_PENALTY=2.194065
PRIMER_LEFT_40_PENALTY=1.501367
PRIMER_RIGHT_40_PENALTY=0.692698
PRIMER_LEFT_40_SEQUENCE=CACTCATCAATTCGTTCCCAG
PRIMER_RIGHT_40_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_40=516,21
PRIMER_RIGHT_40=1447,20
PRIMER_LEFT_40_TM=60.501
PRIMER_RIGHT_40_TM=60.693
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=9.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=932
PRIMER_PAIR_41_PENALTY=2.197515
PRIMER_LEFT_41_PENALTY=1.632424
PRIMER_RIGHT_41_PENALTY=0.565091
PRIMER_LEFT_41_SEQUENCE=CATCCGAGAAATTAAACCCC
PRIMER_RIGHT_41_SEQUENCE=TGTTTGTTTGTTGGAGTGGG
PRIMER_LEFT_41=671,20
PRIMER_RIGHT_41=1460,20
PRIMER_LEFT_41_TM=58.368
PRIMER_RIGHT_41_TM=59.435
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=1.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=10.6000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=790
PRIMER_PAIR_42_PENALTY=2.204190
PRIMER_LEFT_42_PENALTY=1.511492
PRIMER_RIGHT_42_PENALTY=0.692698
PRIMER_LEFT_42_SEQUENCE=TCACTCATCAATTCGTTCCC
PRIMER_RIGHT_42_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_42=515,20
PRIMER_RIGHT_42=1447,20
PRIMER_LEFT_42_TM=58.489
PRIMER_RIGHT_42_TM=60.693
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=933
PRIMER_PAIR_43_PENALTY=2.307294
PRIMER_LEFT_43_PENALTY=0.275749
PRIMER_RIGHT_43_PENALTY=2.031545
PRIMER_LEFT_43_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_43_SEQUENCE=CTCGTTTTCCTGTTCCACCTC
PRIMER_LEFT_43=734,20
PRIMER_RIGHT_43=1719,21
PRIMER_LEFT_43_TM=59.724
PRIMER_RIGHT_43_TM=61.032
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=986
PRIMER_PAIR_44_PENALTY=2.307294
PRIMER_LEFT_44_PENALTY=0.275749
PRIMER_RIGHT_44_PENALTY=2.031545
PRIMER_LEFT_44_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_44_SEQUENCE=TCTCGTTTTCCTGTTCCACCT
PRIMER_LEFT_44=734,20
PRIMER_RIGHT_44=1720,21
PRIMER_LEFT_44_TM=59.724
PRIMER_RIGHT_44_TM=61.032
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=987
PRIMER_PAIR_45_PENALTY=2.314657
PRIMER_LEFT_45_PENALTY=0.639172
PRIMER_RIGHT_45_PENALTY=1.675485
PRIMER_LEFT_45_SEQUENCE=CGTGGGCTTTGTTTTTCTTG
PRIMER_RIGHT_45_SEQUENCE=TGTTGGAGTGGGTATTTTGG
PRIMER_LEFT_45=735,20
PRIMER_RIGHT_45=1452,20
PRIMER_LEFT_45_TM=60.639
PRIMER_RIGHT_45_TM=58.325
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=718
PRIMER_PAIR_46_PENALTY=2.325122
PRIMER_LEFT_46_PENALTY=1.632424
PRIMER_RIGHT_46_PENALTY=0.692698
PRIMER_LEFT_46_SEQUENCE=ATCCGAGAAATTAAACCCCA
PRIMER_RIGHT_46_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_46=672,20
PRIMER_RIGHT_46=1447,20
PRIMER_LEFT_46_TM=58.368
PRIMER_RIGHT_46_TM=60.693
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=11.2000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=776
PRIMER_PAIR_47_PENALTY=2.325122
PRIMER_LEFT_47_PENALTY=1.632424
PRIMER_RIGHT_47_PENALTY=0.692698
PRIMER_LEFT_47_SEQUENCE=CATCCGAGAAATTAAACCCC
PRIMER_RIGHT_47_SEQUENCE=GAGTGGGTATTTTGGCCCTC
PRIMER_LEFT_47=671,20
PRIMER_RIGHT_47=1447,20
PRIMER_LEFT_47_TM=58.368
PRIMER_RIGHT_47_TM=60.693
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=10.6000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=777
PRIMER_PAIR_48_PENALTY=2.341258
PRIMER_LEFT_48_PENALTY=0.275749
PRIMER_RIGHT_48_PENALTY=2.065508
PRIMER_LEFT_48_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_48_SEQUENCE=CATGTTTGTTTGTTGGAGTGG
PRIMER_LEFT_48=734,20
PRIMER_RIGHT_48=1462,21
PRIMER_LEFT_48_TM=59.724
PRIMER_RIGHT_48_TM=58.934
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=2.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=729
PRIMER_PAIR_49_PENALTY=2.341258
PRIMER_LEFT_49_PENALTY=0.275749
PRIMER_RIGHT_49_PENALTY=2.065508
PRIMER_LEFT_49_SEQUENCE=TCGTGGGCTTTGTTTTTCTT
PRIMER_RIGHT_49_SEQUENCE=CCATGTTTGTTTGTTGGAGTG
PRIMER_LEFT_49=734,20
PRIMER_RIGHT_49=1463,21
PRIMER_LEFT_49_TM=59.724
PRIMER_RIGHT_49_TM=58.934
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=730
=
SEQUENCE_ID=MAGIC.433_4_9182654_9182860_wu-0_ler-0_240801_994967_TRUE
SEQUENCE_TARGET=950,306
SEQUENCE_TEMPLATE=ATGAAGAATANGCATANAAAGCGGATAAGGTTGGNNTGGTTAGAAGAATTTCGGACTAAACTTGAANTCAAATGTCTCCCCGTTCGNNTGAGTCCAACCAAATTGTATCGGANANTTTNGACAAATGCAANANNNNTCATNGATTAATNTNGTTTNGGATAATAANNNNATTCANCANAATTATTTTATTATTTTTCATATTACNTNNNNNNATGCATGGAGCTTTGNAGGTNNNNNNNNAGCCATNAATATTTAATACNACCGCAGCCAACTTTTGCAGACTCTGANGGNCTAACCTTTTCTTATANAAAACAGTGTGGATCTNTATAAANNNNACTCTACAATTCGTTATTGGTCATTNCCANAGTAATTTAATTTTNAAAGTTCNGANNNAAACTAAGAGTGGTTCNGTGCCNTTNTCAATGAANGACTCTATTTGTGACAAANCCCTAATATAAACCNTTCCTTCGTTAACTTTCNNNTATCTTTTNTNCNCACACGCATNTCTTCTTNAATGTTCTNTTCTTAGAAAATCATTATTATTCGTCTTTAGTTCTTCACAAAAGAGTCCCGTTTTTCTAGTTNATATTTTGTCCGGGAAAATCAGTTTAAAACTGNAAANATCAGTAATGTCGTCGCTTGTTTCTCNTTGCCAAGTTATAGNATTATTGGTCTTGTTCTNTTTCGNGGTGTNTCTTGCCGGTNAAGACATTCCGGCGAATTTTGTGTTCGGAGATTCATTGGTGGAAGTCGGAAACAACAACTACTTAGCCACATTGGCAAAGGCTAATAATNTTCCTAATGGGATCGATTTTGGATCACCTACTGGTNGATTCACCAACGGAAGAACAATNGTCGATATCNTATGTAAGTNNAGTNGTNACNNAAAAGTTNATAATTCTACGAGTAATANNTTNAANTTAATACACACAAGTTTCCTTGCAATGTTTNAANCCGTAANAATAAAGATTGTATTTNATGTGAAAATNTTANTATNNACNGATNNTTGACTTCCAANATGAAACAACGTTTTCAAATTGTAGCANNNTTTTCGANNGGTTCGTTTTAAAACNAAATTTTNGTTTACTTCATATACGGCTCGATAACNGGAAATTTTGAAAANCGCAGATCAAGCATTAGGTTCCGACGAATTAACTCCACCGTACTTAGCACCAACGACACGCGGACCCCTAATTCTCAACGGCGNCAATTATGCNNCCGGTGGNAGTGGANNCCTCAATTCCNCCGGGNAATTATATGTANGNATATATNTNNNNANNNNNNNNNNGANAAATATATATATATTTNNAAAAATATTATAGATNANTNACGACTCNGTTGGAATAAAGANCTCGTACCGACTCCCTATTGCAACGGTTGTTGATGGNGGTGANNNNNNNNCTNTAATCTTTAGTTCCTNAGGTANNNNATTTAATTTGGGCCATTNTANTCTTGTAATNTTNAGNCNCTTTTACTCGTTAAACACTATGAGGATTGTAANNNNNNTGGAGTTTAGACAAAANAATAANAAAAGNACACGAATGGANNCTCTCGAANGNTGNANTTTTGTTGGAGTTTTGACAAAACAAANCNNTTATCTTNGCGATTGCTTTTGTCATTTGTTANTTTNGTTTCNTTAGTTAACAGGGNCCGCNCAATTAATTTAATTTCNTTACACTTGTACCAACTNCTTGATTCTGNTTAGGACATGATAGGTATTANTTCTGAAATTTTAGACTCGAGTGTGTGTTGAATTNGANCTTCAGTTCCACAAGTTTCTCTACAATAGACTCATTTCAGACTNGTTCTATGTCTTATTTGATCGGTTTACAGCAAATACTAATGGAGTCAAGATGNTANGNNNNNTTNTTTTTTTNCGGTTCNATATAGATCAACGAATTTAGATTCGTGGAGCCCTGAGCAGCTTAAGGCAATGATGTTTGGTGNAAANTACCTTGCTCNGGTGTTNTTCAAGANAANNNATGGANAGACGATNGCAATANCGAGTCTAANTACACTTTCAAGAGCTGCTAATNTGTATNGGCAGATTCTTGCTAANGAAGTTGCTAAAGCCATTGCACAAGATNCTACCACTGATCTACCAAAAGCATCTCATGGGGNTTCTTCNTCTTCTGTCAAGGAAGAGTTANGTCCCAAAGAAACTTNTTTCTGTGACTTTTANGGTCGANCCATAGTTTNCCCCATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.649992
PRIMER_LEFT_0_PENALTY=0.317359
PRIMER_RIGHT_0_PENALTY=2.332633
PRIMER_LEFT_0_SEQUENCE=GATTCATTGGTGGAAGTCGG
PRIMER_RIGHT_0_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_0=734,20
PRIMER_RIGHT_0=1375,20
PRIMER_LEFT_0_TM=60.317
PRIMER_RIGHT_0_TM=62.333
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=9.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=642
PRIMER_PAIR_1_PENALTY=2.649992
PRIMER_LEFT_1_PENALTY=0.317359
PRIMER_RIGHT_1_PENALTY=2.332633
PRIMER_LEFT_1_SEQUENCE=GTTCGGAGATTCATTGGTGG
PRIMER_RIGHT_1_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_1=727,20
PRIMER_RIGHT_1=1375,20
PRIMER_LEFT_1_TM=60.317
PRIMER_RIGHT_1_TM=62.333
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=649
PRIMER_PAIR_2_PENALTY=2.685757
PRIMER_LEFT_2_PENALTY=0.353123
PRIMER_RIGHT_2_PENALTY=2.332633
PRIMER_LEFT_2_SEQUENCE=ATTGGTGGAAGTCGGAAACA
PRIMER_RIGHT_2_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_2=739,20
PRIMER_RIGHT_2=1375,20
PRIMER_LEFT_2_TM=60.353
PRIMER_RIGHT_2_TM=62.333
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=637
PRIMER_PAIR_3_PENALTY=2.685757
PRIMER_LEFT_3_PENALTY=0.353123
PRIMER_RIGHT_3_PENALTY=2.332633
PRIMER_LEFT_3_SEQUENCE=CATTGGTGGAAGTCGGAAAC
PRIMER_RIGHT_3_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_3=738,20
PRIMER_RIGHT_3=1375,20
PRIMER_LEFT_3_TM=60.353
PRIMER_RIGHT_3_TM=62.333
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=638
PRIMER_PAIR_4_PENALTY=2.784452
PRIMER_LEFT_4_PENALTY=0.451819
PRIMER_RIGHT_4_PENALTY=2.332633
PRIMER_LEFT_4_SEQUENCE=CGGAGATTCATTGGTGGAAG
PRIMER_RIGHT_4_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_4=730,20
PRIMER_RIGHT_4=1375,20
PRIMER_LEFT_4_TM=60.452
PRIMER_RIGHT_4_TM=62.333
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=646
PRIMER_PAIR_5_PENALTY=2.836339
PRIMER_LEFT_5_PENALTY=0.503705
PRIMER_RIGHT_5_PENALTY=2.332633
PRIMER_LEFT_5_SEQUENCE=TGTTCGGAGATTCATTGGTG
PRIMER_RIGHT_5_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_5=726,20
PRIMER_RIGHT_5=1375,20
PRIMER_LEFT_5_TM=59.496
PRIMER_RIGHT_5_TM=62.333
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=650
PRIMER_PAIR_6_PENALTY=2.836339
PRIMER_LEFT_6_PENALTY=0.503705
PRIMER_RIGHT_6_PENALTY=2.332633
PRIMER_LEFT_6_SEQUENCE=TGTGTTCGGAGATTCATTGG
PRIMER_RIGHT_6_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_6=724,20
PRIMER_RIGHT_6=1375,20
PRIMER_LEFT_6_TM=59.496
PRIMER_RIGHT_6_TM=62.333
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=652
PRIMER_PAIR_7_PENALTY=3.266237
PRIMER_LEFT_7_PENALTY=0.933604
PRIMER_RIGHT_7_PENALTY=2.332633
PRIMER_LEFT_7_SEQUENCE=GGTGGAAGTCGGAAACAACA
PRIMER_RIGHT_7_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_7=742,20
PRIMER_RIGHT_7=1375,20
PRIMER_LEFT_7_TM=60.934
PRIMER_RIGHT_7_TM=62.333
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=634
PRIMER_PAIR_8_PENALTY=3.266237
PRIMER_LEFT_8_PENALTY=0.933604
PRIMER_RIGHT_8_PENALTY=2.332633
PRIMER_LEFT_8_SEQUENCE=TGGTGGAAGTCGGAAACAAC
PRIMER_RIGHT_8_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_8=741,20
PRIMER_RIGHT_8=1375,20
PRIMER_LEFT_8_TM=60.934
PRIMER_RIGHT_8_TM=62.333
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=635
PRIMER_PAIR_9_PENALTY=3.369651
PRIMER_LEFT_9_PENALTY=1.037017
PRIMER_RIGHT_9_PENALTY=2.332633
PRIMER_LEFT_9_SEQUENCE=GATTCACCAACGGAAGAACAA
PRIMER_RIGHT_9_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_9=831,21
PRIMER_RIGHT_9=1375,20
PRIMER_LEFT_9_TM=59.963
PRIMER_RIGHT_9_TM=62.333
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=545
PRIMER_PAIR_10_PENALTY=3.543149
PRIMER_LEFT_10_PENALTY=1.210515
PRIMER_RIGHT_10_PENALTY=2.332633
PRIMER_LEFT_10_SEQUENCE=GGATCGATTTTGGATCACCT
PRIMER_RIGHT_10_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_10=804,20
PRIMER_RIGHT_10=1375,20
PRIMER_LEFT_10_TM=58.789
PRIMER_RIGHT_10_TM=62.333
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=572
PRIMER_PAIR_11_PENALTY=3.701083
PRIMER_LEFT_11_PENALTY=1.368450
PRIMER_RIGHT_11_PENALTY=2.332633
PRIMER_LEFT_11_SEQUENCE=GTGTTCGGAGATTCATTGGTG
PRIMER_RIGHT_11_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_11=725,21
PRIMER_RIGHT_11=1375,20
PRIMER_LEFT_11_TM=60.368
PRIMER_RIGHT_11_TM=62.333
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=651
PRIMER_PAIR_12_PENALTY=3.701083
PRIMER_LEFT_12_PENALTY=1.368450
PRIMER_RIGHT_12_PENALTY=2.332633
PRIMER_LEFT_12_SEQUENCE=TGTGTTCGGAGATTCATTGGT
PRIMER_RIGHT_12_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_12=724,21
PRIMER_RIGHT_12=1375,20
PRIMER_LEFT_12_TM=60.368
PRIMER_RIGHT_12_TM=62.333
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=652
PRIMER_PAIR_13_PENALTY=3.708260
PRIMER_LEFT_13_PENALTY=1.375627
PRIMER_RIGHT_13_PENALTY=2.332633
PRIMER_LEFT_13_SEQUENCE=TGGAAGTCGGAAACAACAACT
PRIMER_RIGHT_13_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_13=744,21
PRIMER_RIGHT_13=1375,20
PRIMER_LEFT_13_TM=59.624
PRIMER_RIGHT_13_TM=62.333
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=632
PRIMER_PAIR_14_PENALTY=3.723050
PRIMER_LEFT_14_PENALTY=1.390417
PRIMER_RIGHT_14_PENALTY=2.332633
PRIMER_LEFT_14_SEQUENCE=TCGGAGATTCATTGGTGGAA
PRIMER_RIGHT_14_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_14=729,20
PRIMER_RIGHT_14=1375,20
PRIMER_LEFT_14_TM=61.390
PRIMER_RIGHT_14_TM=62.333
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=647
PRIMER_PAIR_15_PENALTY=3.723050
PRIMER_LEFT_15_PENALTY=1.390417
PRIMER_RIGHT_15_PENALTY=2.332633
PRIMER_LEFT_15_SEQUENCE=TTCGGAGATTCATTGGTGGA
PRIMER_RIGHT_15_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_15=728,20
PRIMER_RIGHT_15=1375,20
PRIMER_LEFT_15_TM=61.390
PRIMER_RIGHT_15_TM=62.333
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=648
PRIMER_PAIR_16_PENALTY=3.736822
PRIMER_LEFT_16_PENALTY=1.404189
PRIMER_RIGHT_16_PENALTY=2.332633
PRIMER_LEFT_16_SEQUENCE=TGGAAGTCGGAAACAACAAC
PRIMER_RIGHT_16_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_16=744,20
PRIMER_RIGHT_16=1375,20
PRIMER_LEFT_16_TM=58.596
PRIMER_RIGHT_16_TM=62.333
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=632
PRIMER_PAIR_17_PENALTY=3.736822
PRIMER_LEFT_17_PENALTY=1.404189
PRIMER_RIGHT_17_PENALTY=2.332633
PRIMER_LEFT_17_SEQUENCE=GTGGAAGTCGGAAACAACAA
PRIMER_RIGHT_17_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_17=743,20
PRIMER_RIGHT_17=1375,20
PRIMER_LEFT_17_TM=58.596
PRIMER_RIGHT_17_TM=62.333
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=633
PRIMER_PAIR_18_PENALTY=3.749233
PRIMER_LEFT_18_PENALTY=1.416599
PRIMER_RIGHT_18_PENALTY=2.332633
PRIMER_LEFT_18_SEQUENCE=TCATTGGTGGAAGTCGGAAA
PRIMER_RIGHT_18_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_18=737,20
PRIMER_RIGHT_18=1375,20
PRIMER_LEFT_18_TM=61.417
PRIMER_RIGHT_18_TM=62.333
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=9.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=639
PRIMER_PAIR_19_PENALTY=3.749233
PRIMER_LEFT_19_PENALTY=1.416599
PRIMER_RIGHT_19_PENALTY=2.332633
PRIMER_LEFT_19_SEQUENCE=TTCATTGGTGGAAGTCGGAA
PRIMER_RIGHT_19_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_19=736,20
PRIMER_RIGHT_19=1375,20
PRIMER_LEFT_19_TM=61.417
PRIMER_RIGHT_19_TM=62.333
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=10.2000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=640
PRIMER_PAIR_20_PENALTY=3.774936
PRIMER_LEFT_20_PENALTY=1.442303
PRIMER_RIGHT_20_PENALTY=2.332633
PRIMER_LEFT_20_SEQUENCE=TTGGTGGAAGTCGGAAACAA
PRIMER_RIGHT_20_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_20=740,20
PRIMER_RIGHT_20=1375,20
PRIMER_LEFT_20_TM=61.442
PRIMER_RIGHT_20_TM=62.333
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=9.6000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=636
PRIMER_PAIR_21_PENALTY=3.789968
PRIMER_LEFT_21_PENALTY=1.457334
PRIMER_RIGHT_21_PENALTY=2.332633
PRIMER_LEFT_21_SEQUENCE=GATTCACCAACGGAAGAACA
PRIMER_RIGHT_21_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_21=831,20
PRIMER_RIGHT_21=1375,20
PRIMER_LEFT_21_TM=58.543
PRIMER_RIGHT_21_TM=62.333
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=545
PRIMER_PAIR_22_PENALTY=3.845672
PRIMER_LEFT_22_PENALTY=1.513039
PRIMER_RIGHT_22_PENALTY=2.332633
PRIMER_LEFT_22_SEQUENCE=GTGGAAGTCGGAAACAACAAC
PRIMER_RIGHT_22_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_22=743,21
PRIMER_RIGHT_22=1375,20
PRIMER_LEFT_22_TM=59.487
PRIMER_RIGHT_22_TM=62.333
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=9.6000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=633
PRIMER_PAIR_23_PENALTY=3.894156
PRIMER_LEFT_23_PENALTY=1.561523
PRIMER_RIGHT_23_PENALTY=2.332633
PRIMER_LEFT_23_SEQUENCE=CGATTTTGGATCACCTACTGG
PRIMER_RIGHT_23_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_23=808,21
PRIMER_RIGHT_23=1375,20
PRIMER_LEFT_23_TM=59.438
PRIMER_RIGHT_23_TM=62.333
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=568
PRIMER_PAIR_24_PENALTY=3.909523
PRIMER_LEFT_24_PENALTY=1.576890
PRIMER_RIGHT_24_PENALTY=2.332633
PRIMER_LEFT_24_SEQUENCE=ATTCCGGCGAATTTTGTGTT
PRIMER_RIGHT_24_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_24=710,20
PRIMER_RIGHT_24=1375,20
PRIMER_LEFT_24_TM=61.577
PRIMER_RIGHT_24_TM=62.333
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=666
PRIMER_PAIR_25_PENALTY=4.211127
PRIMER_LEFT_25_PENALTY=1.878494
PRIMER_RIGHT_25_PENALTY=2.332633
PRIMER_LEFT_25_SEQUENCE=TTGTGTTCGGAGATTCATTGG
PRIMER_RIGHT_25_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_25=723,21
PRIMER_RIGHT_25=1375,20
PRIMER_LEFT_25_TM=60.878
PRIMER_RIGHT_25_TM=62.333
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=653
PRIMER_PAIR_26_PENALTY=4.284453
PRIMER_LEFT_26_PENALTY=1.951820
PRIMER_RIGHT_26_PENALTY=2.332633
PRIMER_LEFT_26_SEQUENCE=ATTCACCAACGGAAGAACAA
PRIMER_RIGHT_26_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_26=832,20
PRIMER_RIGHT_26=1375,20
PRIMER_LEFT_26_TM=58.048
PRIMER_RIGHT_26_TM=62.333
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=544
PRIMER_PAIR_27_PENALTY=4.320442
PRIMER_LEFT_27_PENALTY=1.987809
PRIMER_RIGHT_27_PENALTY=2.332633
PRIMER_LEFT_27_SEQUENCE=CCTAATGGGATCGATTTTGGA
PRIMER_RIGHT_27_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_27=797,21
PRIMER_RIGHT_27=1375,20
PRIMER_LEFT_27_TM=60.988
PRIMER_RIGHT_27_TM=62.333
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=579
PRIMER_PAIR_28_PENALTY=4.382371
PRIMER_LEFT_28_PENALTY=2.049738
PRIMER_RIGHT_28_PENALTY=2.332633
PRIMER_LEFT_28_SEQUENCE=AATGTCGTCGCTTGTTTCTC
PRIMER_RIGHT_28_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_28=628,20
PRIMER_RIGHT_28=1375,20
PRIMER_LEFT_28_TM=57.950
PRIMER_RIGHT_28_TM=62.333
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=748
PRIMER_PAIR_29_PENALTY=4.444594
PRIMER_LEFT_29_PENALTY=2.111961
PRIMER_RIGHT_29_PENALTY=2.332633
PRIMER_LEFT_29_SEQUENCE=GACATTCCGGCGAATTTTGT
PRIMER_RIGHT_29_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_29=707,20
PRIMER_RIGHT_29=1375,20
PRIMER_LEFT_29_TM=62.112
PRIMER_RIGHT_29_TM=62.333
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=669
PRIMER_PAIR_30_PENALTY=4.506623
PRIMER_LEFT_30_PENALTY=2.173990
PRIMER_RIGHT_30_PENALTY=2.332633
PRIMER_LEFT_30_SEQUENCE=TTCTTCACAAAAGAGTCCCGT
PRIMER_RIGHT_30_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_30=553,21
PRIMER_RIGHT_30=1375,20
PRIMER_LEFT_30_TM=58.826
PRIMER_RIGHT_30_TM=62.333
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=11.1000
PRIMER_RIGHT_30_END_STABILITY=9.6000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=823
PRIMER_PAIR_31_PENALTY=4.519959
PRIMER_LEFT_31_PENALTY=2.187326
PRIMER_RIGHT_31_PENALTY=2.332633
PRIMER_LEFT_31_SEQUENCE=CAGTAATGTCGTCGCTTGTTTC
PRIMER_RIGHT_31_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_31=624,22
PRIMER_RIGHT_31=1375,20
PRIMER_LEFT_31_TM=59.813
PRIMER_RIGHT_31_TM=62.333
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=752
PRIMER_PAIR_32_PENALTY=4.519959
PRIMER_LEFT_32_PENALTY=2.187326
PRIMER_RIGHT_32_PENALTY=2.332633
PRIMER_LEFT_32_SEQUENCE=TCAGTAATGTCGTCGCTTGTTT
PRIMER_RIGHT_32_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_32=623,22
PRIMER_RIGHT_32=1375,20
PRIMER_LEFT_32_TM=59.813
PRIMER_RIGHT_32_TM=62.333
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=753
PRIMER_PAIR_33_PENALTY=4.585471
PRIMER_LEFT_33_PENALTY=2.252838
PRIMER_RIGHT_33_PENALTY=2.332633
PRIMER_LEFT_33_SEQUENCE=AGATTCATTGGTGGAAGTCGG
PRIMER_RIGHT_33_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_33=733,21
PRIMER_RIGHT_33=1375,20
PRIMER_LEFT_33_TM=61.253
PRIMER_RIGHT_33_TM=62.333
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.6000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=643
PRIMER_PAIR_34_PENALTY=4.585471
PRIMER_LEFT_34_PENALTY=2.252838
PRIMER_RIGHT_34_PENALTY=2.332633
PRIMER_LEFT_34_SEQUENCE=CGGAGATTCATTGGTGGAAGT
PRIMER_RIGHT_34_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_34=730,21
PRIMER_RIGHT_34=1375,20
PRIMER_LEFT_34_TM=61.253
PRIMER_RIGHT_34_TM=62.333
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=646
PRIMER_PAIR_35_PENALTY=4.672448
PRIMER_LEFT_35_PENALTY=2.339815
PRIMER_RIGHT_35_PENALTY=2.332633
PRIMER_LEFT_35_SEQUENCE=GTTCTTCACAAAAGAGTCCCGT
PRIMER_RIGHT_35_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_35=552,22
PRIMER_RIGHT_35=1375,20
PRIMER_LEFT_35_TM=59.660
PRIMER_RIGHT_35_TM=62.333
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=11.1000
PRIMER_RIGHT_35_END_STABILITY=9.6000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=824
PRIMER_PAIR_36_PENALTY=4.768355
PRIMER_LEFT_36_PENALTY=2.435722
PRIMER_RIGHT_36_PENALTY=2.332633
PRIMER_LEFT_36_SEQUENCE=GTGGAAGTCGGAAACAACAACT
PRIMER_RIGHT_36_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_36=743,22
PRIMER_RIGHT_36=1375,20
PRIMER_LEFT_36_TM=60.436
PRIMER_RIGHT_36_TM=62.333
PRIMER_LEFT_36_GC_PERCENT=45.455
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=9.6000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=633
PRIMER_PAIR_37_PENALTY=4.840951
PRIMER_LEFT_37_PENALTY=2.508317
PRIMER_RIGHT_37_PENALTY=2.332633
PRIMER_LEFT_37_SEQUENCE=CTAATGGGATCGATTTTGGA
PRIMER_RIGHT_37_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_37=798,20
PRIMER_RIGHT_37=1375,20
PRIMER_LEFT_37_TM=57.492
PRIMER_RIGHT_37_TM=62.333
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=578
PRIMER_PAIR_38_PENALTY=4.886824
PRIMER_LEFT_38_PENALTY=2.554191
PRIMER_RIGHT_38_PENALTY=2.332633
PRIMER_LEFT_38_SEQUENCE=TCAGTAATGTCGTCGCTTGTT
PRIMER_RIGHT_38_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_38=623,21
PRIMER_RIGHT_38=1375,20
PRIMER_LEFT_38_TM=58.446
PRIMER_RIGHT_38_TM=62.333
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=753
PRIMER_PAIR_39_PENALTY=4.914647
PRIMER_LEFT_39_PENALTY=2.582014
PRIMER_RIGHT_39_PENALTY=2.332633
PRIMER_LEFT_39_SEQUENCE=GTGTTCGGAGATTCATTGGT
PRIMER_RIGHT_39_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_39=725,20
PRIMER_RIGHT_39=1375,20
PRIMER_LEFT_39_TM=57.418
PRIMER_RIGHT_39_TM=62.333
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=651
PRIMER_PAIR_40_PENALTY=4.945640
PRIMER_LEFT_40_PENALTY=2.613007
PRIMER_RIGHT_40_PENALTY=2.332633
PRIMER_LEFT_40_SEQUENCE=ATTCATTGGTGGAAGTCGGAA
PRIMER_RIGHT_40_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_40=735,21
PRIMER_RIGHT_40=1375,20
PRIMER_LEFT_40_TM=61.613
PRIMER_RIGHT_40_TM=62.333
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=10.2000
PRIMER_RIGHT_40_END_STABILITY=9.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=641
PRIMER_PAIR_41_PENALTY=4.968279
PRIMER_LEFT_41_PENALTY=2.635645
PRIMER_RIGHT_41_PENALTY=2.332633
PRIMER_LEFT_41_SEQUENCE=ATTGGTGGAAGTCGGAAACAA
PRIMER_RIGHT_41_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_41=739,21
PRIMER_RIGHT_41=1375,20
PRIMER_LEFT_41_TM=61.636
PRIMER_RIGHT_41_TM=62.333
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=637
PRIMER_PAIR_42_PENALTY=4.975904
PRIMER_LEFT_42_PENALTY=2.643271
PRIMER_RIGHT_42_PENALTY=2.332633
PRIMER_LEFT_42_SEQUENCE=TCTTCACAAAAGAGTCCCGT
PRIMER_RIGHT_42_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_42=554,20
PRIMER_RIGHT_42=1375,20
PRIMER_LEFT_42_TM=57.357
PRIMER_RIGHT_42_TM=62.333
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=11.1000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=822
PRIMER_PAIR_43_PENALTY=5.128128
PRIMER_LEFT_43_PENALTY=2.795495
PRIMER_RIGHT_43_PENALTY=2.332633
PRIMER_LEFT_43_SEQUENCE=GGCGAATTTTGTGTTCGGAG
PRIMER_RIGHT_43_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_43=715,20
PRIMER_RIGHT_43=1375,20
PRIMER_LEFT_43_TM=62.795
PRIMER_RIGHT_43_TM=62.333
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=661
PRIMER_PAIR_44_PENALTY=5.250359
PRIMER_LEFT_44_PENALTY=0.317359
PRIMER_RIGHT_44_PENALTY=4.933000
PRIMER_LEFT_44_SEQUENCE=GATTCATTGGTGGAAGTCGG
PRIMER_RIGHT_44_SEQUENCE=CCGTTGCAATAGGGAGTCGG
PRIMER_LEFT_44=734,20
PRIMER_RIGHT_44=1376,20
PRIMER_LEFT_44_TM=60.317
PRIMER_RIGHT_44_TM=64.933
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=60.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=9.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=643
PRIMER_PAIR_45_PENALTY=5.250359
PRIMER_LEFT_45_PENALTY=0.317359
PRIMER_RIGHT_45_PENALTY=4.933000
PRIMER_LEFT_45_SEQUENCE=GTTCGGAGATTCATTGGTGG
PRIMER_RIGHT_45_SEQUENCE=CCGTTGCAATAGGGAGTCGG
PRIMER_LEFT_45=727,20
PRIMER_RIGHT_45=1376,20
PRIMER_LEFT_45_TM=60.317
PRIMER_RIGHT_45_TM=64.933
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=60.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=9.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=650
PRIMER_PAIR_46_PENALTY=5.286123
PRIMER_LEFT_46_PENALTY=0.353123
PRIMER_RIGHT_46_PENALTY=4.933000
PRIMER_LEFT_46_SEQUENCE=ATTGGTGGAAGTCGGAAACA
PRIMER_RIGHT_46_SEQUENCE=CCGTTGCAATAGGGAGTCGG
PRIMER_LEFT_46=739,20
PRIMER_RIGHT_46=1376,20
PRIMER_LEFT_46_TM=60.353
PRIMER_RIGHT_46_TM=64.933
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=60.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=9.6000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=638
PRIMER_PAIR_47_PENALTY=5.286123
PRIMER_LEFT_47_PENALTY=0.353123
PRIMER_RIGHT_47_PENALTY=4.933000
PRIMER_LEFT_47_SEQUENCE=CATTGGTGGAAGTCGGAAAC
PRIMER_RIGHT_47_SEQUENCE=CCGTTGCAATAGGGAGTCGG
PRIMER_LEFT_47=738,20
PRIMER_RIGHT_47=1376,20
PRIMER_LEFT_47_TM=60.353
PRIMER_RIGHT_47_TM=64.933
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=60.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=9.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=639
PRIMER_PAIR_48_PENALTY=5.292661
PRIMER_LEFT_48_PENALTY=2.960028
PRIMER_RIGHT_48_PENALTY=2.332633
PRIMER_LEFT_48_SEQUENCE=TAATGTCGTCGCTTGTTTCTC
PRIMER_RIGHT_48_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_48=627,21
PRIMER_RIGHT_48=1375,20
PRIMER_LEFT_48_TM=58.040
PRIMER_RIGHT_48_TM=62.333
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=749
PRIMER_PAIR_49_PENALTY=5.358006
PRIMER_LEFT_49_PENALTY=3.025372
PRIMER_RIGHT_49_PENALTY=2.332633
PRIMER_LEFT_49_SEQUENCE=CAGTAATGTCGTCGCTTGTTT
PRIMER_RIGHT_49_SEQUENCE=CGTTGCAATAGGGAGTCGGT
PRIMER_LEFT_49=624,21
PRIMER_RIGHT_49=1375,20
PRIMER_LEFT_49_TM=57.975
PRIMER_RIGHT_49_TM=62.333
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=752
=
SEQUENCE_ID=MAGIC.446_5_17372085_17372725_po-0_tsu-0_4803_6905_TRUE
SEQUENCE_TARGET=950,740
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCAAGAAGCTGTCAAAACACTGTCCCTGGTAGGANTATTTTTCATTGCCGGAGCTACCACATCTTCTAGAAGCCCTTGTTGCGGCCGACACTGATCAANCGGNAAAAACAATCCCTCTTCGAGATTTGCCGGTGTGTTTTCTTGGGCTTTATTGACTGGATGATTTTGGTTGGNGAGCTCACCTAATTGTGCTTCTTTGCTCTCTTCTTGGGNTTGAGATTTTTTGTTCATATCTTGAGCATTATGTTTATGTATTTTCGTTTTTTTATTGGGCCATTGACGTGTCTTATCGGGTAGGAAGAAAGGGTAAGTATAGTGGAAGTGGAAACCTTCGTAGGTAANGATATATGTGTTAGACTCATCAATTGATCTCTCCACTTGCTTCTTTGCATTGCATATTGGGTTTGTGCACTTGTAATAACTCCTAGACATANATCACAAATATAGTAACATTAGGNTTGTNACCGAAAACTAAGTATACTAAATAAAACTTCCATATGTTGATTTTAATACGTATCATTTTGGCCGAACCAAAGAAGTTCGATNTGCATATACAATTNTAAAACGGAGAATGCATANNNNTACCTTGGGTTAGGGCTATTTTTGATTGATTTTTGGCCATATTTTCTCCATTTGTATCCATCATCACACATTCCATTACTATTGTTCTTCACCTTTAAAGTGTACCTATCCACCNTGCTCAAAGTACTTCTTTNCAAAAGAGGAACTCTATACATANNTATNANGNAACNCATNAGTACTTATTATTAAAGGGCTATACTAAACATAAGGNAAAGAGATGTANNNNNNNNNNNNNNNNNNNNNNNNTCNATTACTTACGTTGATTTNGAGATTTCTNGGACTGGGTGGGTCNGGGGTTCNACNAAGGCCAGAGCGTTTGCAATATCTTNGATTCGTGGACCGGAGTAAAGTGTTGATGTGACGAANTTGNTGGCAATGAAATCTTCATTTGGCATGAGCCCATGCATATGATGTTGGTCTTGAGGCAGAAANAAAAGAGGTGGCTCNTAAANANAAGGATCTTCAACGGCGTTNTTTCCACACCTTCTTGNCCACTGANAACCTTCTTCTTCCATATGAGTATGGNGACAAGAAAANTTATGCGAGCTGGTGGTNNNNNNNNGAGGGGGAGAGNGAGAGAGNGATTCTTTNTTCTTTTAACNNTTAAGAGTAACGTGTGTTNAATTTCTATTNNNTTNNNTTTTTTTGAGGAGGGGATCTACACGAGTTATATATAATACCAAAATTAGNCATGTGNATTCANTTTTGTTATANTTGAGTTATATGCCATATTATTGTTTNNAATAAATTATCTACANTAATTANTGTGTTGAATTTATATCAANTTATCTTTTATCTCTAAGTTNTGNNTNGCACAACATTATTATAATCGTTAANNAACATAAAAACACTATTATCATTGATTTTTACTTTNTGGAATGTCCACCATCTTTTCACACGNATCGATTTACACTAGTTTAGNGATAGNNTTGGAGGAATCCAAATAGTTTAGATATGAAATGTATATATGTATAAATGNNTTTTTTTTTTGAGCTTCTAACTTGTTGCTTTAANAAANNNNNNNNTATCNAGAACTATTNTTTATACCTAATNNAAANNTTNACCTCATTAGATTTGGCATCNANANTTNGGACGCNGNAAANTNAAGTTTTCTAACTTCAGAGAGTGAGNCANANNTAGGATTCCGTGNTTGAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.858593
PRIMER_LEFT_0_PENALTY=1.450652
PRIMER_RIGHT_0_PENALTY=0.407941
PRIMER_LEFT_0_SEQUENCE=TGTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_0_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_0=911,21
PRIMER_RIGHT_0=1834,20
PRIMER_LEFT_0_TM=60.451
PRIMER_RIGHT_0_TM=59.592
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=924
PRIMER_PAIR_1_PENALTY=2.146034
PRIMER_LEFT_1_PENALTY=1.738092
PRIMER_RIGHT_1_PENALTY=0.407941
PRIMER_LEFT_1_SEQUENCE=GCTGTCAAAACACTGTCCCTG
PRIMER_RIGHT_1_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_1=909,21
PRIMER_RIGHT_1=1834,20
PRIMER_LEFT_1_TM=60.738
PRIMER_RIGHT_1_TM=59.592
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=926
PRIMER_PAIR_2_PENALTY=2.161300
PRIMER_LEFT_2_PENALTY=1.753359
PRIMER_RIGHT_2_PENALTY=0.407941
PRIMER_LEFT_2_SEQUENCE=AAGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_2_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_2=907,21
PRIMER_RIGHT_2=1834,20
PRIMER_LEFT_2_TM=59.247
PRIMER_RIGHT_2_TM=59.592
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=928
PRIMER_PAIR_3_PENALTY=2.534762
PRIMER_LEFT_3_PENALTY=1.450652
PRIMER_RIGHT_3_PENALTY=1.084110
PRIMER_LEFT_3_SEQUENCE=TGTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_3_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_3=911,21
PRIMER_RIGHT_3=1835,20
PRIMER_LEFT_3_TM=60.451
PRIMER_RIGHT_3_TM=61.084
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=925
PRIMER_PAIR_4_PENALTY=2.550312
PRIMER_LEFT_4_PENALTY=2.142370
PRIMER_RIGHT_4_PENALTY=0.407941
PRIMER_LEFT_4_SEQUENCE=AGCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_4_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_4=908,21
PRIMER_RIGHT_4=1834,20
PRIMER_LEFT_4_TM=58.858
PRIMER_RIGHT_4_TM=59.592
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=927
PRIMER_PAIR_5_PENALTY=2.607516
PRIMER_LEFT_5_PENALTY=2.438044
PRIMER_RIGHT_5_PENALTY=0.169471
PRIMER_LEFT_5_SEQUENCE=AAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_5_SEQUENCE=CTGCCTCAAGACCAACATCA
PRIMER_LEFT_5=915,21
PRIMER_RIGHT_5=1911,20
PRIMER_LEFT_5_TM=58.562
PRIMER_RIGHT_5_TM=59.831
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=997
PRIMER_PAIR_6_PENALTY=2.617284
PRIMER_LEFT_6_PENALTY=2.209343
PRIMER_RIGHT_6_PENALTY=0.407941
PRIMER_LEFT_6_SEQUENCE=AAGCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_6_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_6=907,22
PRIMER_RIGHT_6=1834,20
PRIMER_LEFT_6_TM=60.209
PRIMER_RIGHT_6_TM=59.592
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=928
PRIMER_PAIR_7_PENALTY=2.638228
PRIMER_LEFT_7_PENALTY=2.230287
PRIMER_RIGHT_7_PENALTY=0.407941
PRIMER_LEFT_7_SEQUENCE=GCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_7_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_7=909,20
PRIMER_RIGHT_7=1834,20
PRIMER_LEFT_7_TM=57.770
PRIMER_RIGHT_7_TM=59.592
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=926
PRIMER_PAIR_8_PENALTY=2.638228
PRIMER_LEFT_8_PENALTY=2.230287
PRIMER_RIGHT_8_PENALTY=0.407941
PRIMER_LEFT_8_SEQUENCE=AGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_8_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_8=908,20
PRIMER_RIGHT_8=1834,20
PRIMER_LEFT_8_TM=57.770
PRIMER_RIGHT_8_TM=59.592
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.1000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=927
PRIMER_PAIR_9_PENALTY=2.722701
PRIMER_LEFT_9_PENALTY=2.553229
PRIMER_RIGHT_9_PENALTY=0.169471
PRIMER_LEFT_9_SEQUENCE=GTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_9_SEQUENCE=CTGCCTCAAGACCAACATCA
PRIMER_LEFT_9=912,20
PRIMER_RIGHT_9=1911,20
PRIMER_LEFT_9_TM=57.447
PRIMER_RIGHT_9_TM=59.831
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=1000
PRIMER_PAIR_10_PENALTY=2.822203
PRIMER_LEFT_10_PENALTY=1.738092
PRIMER_RIGHT_10_PENALTY=1.084110
PRIMER_LEFT_10_SEQUENCE=GCTGTCAAAACACTGTCCCTG
PRIMER_RIGHT_10_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_10=909,21
PRIMER_RIGHT_10=1835,20
PRIMER_LEFT_10_TM=60.738
PRIMER_RIGHT_10_TM=61.084
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=927
PRIMER_PAIR_11_PENALTY=2.829506
PRIMER_LEFT_11_PENALTY=1.450652
PRIMER_RIGHT_11_PENALTY=1.378854
PRIMER_LEFT_11_SEQUENCE=TGTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_11_SEQUENCE=CAACACTTTACTCCGGTCCA
PRIMER_LEFT_11=911,21
PRIMER_RIGHT_11=1838,20
PRIMER_LEFT_11_TM=60.451
PRIMER_RIGHT_11_TM=58.621
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=928
PRIMER_PAIR_12_PENALTY=2.837469
PRIMER_LEFT_12_PENALTY=1.753359
PRIMER_RIGHT_12_PENALTY=1.084110
PRIMER_LEFT_12_SEQUENCE=AAGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_12_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_12=907,21
PRIMER_RIGHT_12=1835,20
PRIMER_LEFT_12_TM=59.247
PRIMER_RIGHT_12_TM=61.084
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.1000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=929
PRIMER_PAIR_13_PENALTY=2.845985
PRIMER_LEFT_13_PENALTY=2.438044
PRIMER_RIGHT_13_PENALTY=0.407941
PRIMER_LEFT_13_SEQUENCE=AAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_13_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_13=915,21
PRIMER_RIGHT_13=1834,20
PRIMER_LEFT_13_TM=58.562
PRIMER_RIGHT_13_TM=59.592
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.2000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=920
PRIMER_PAIR_14_PENALTY=2.961170
PRIMER_LEFT_14_PENALTY=2.553229
PRIMER_RIGHT_14_PENALTY=0.407941
PRIMER_LEFT_14_SEQUENCE=GTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_14_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_14=912,20
PRIMER_RIGHT_14=1834,20
PRIMER_LEFT_14_TM=57.447
PRIMER_RIGHT_14_TM=59.592
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=923
PRIMER_PAIR_15_PENALTY=2.985542
PRIMER_LEFT_15_PENALTY=1.450652
PRIMER_RIGHT_15_PENALTY=1.534890
PRIMER_LEFT_15_SEQUENCE=TGTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_15_SEQUENCE=TCAACACTTTACTCCGGTCCA
PRIMER_LEFT_15=911,21
PRIMER_RIGHT_15=1839,21
PRIMER_LEFT_15_TM=60.451
PRIMER_RIGHT_15_TM=60.535
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=929
PRIMER_PAIR_16_PENALTY=3.023260
PRIMER_LEFT_16_PENALTY=2.438044
PRIMER_RIGHT_16_PENALTY=0.585216
PRIMER_LEFT_16_SEQUENCE=AAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_16_SEQUENCE=TTTCTGCCTCAAGACCAACA
PRIMER_LEFT_16=915,21
PRIMER_RIGHT_16=1914,20
PRIMER_LEFT_16_TM=58.562
PRIMER_RIGHT_16_TM=59.415
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=1000
PRIMER_PAIR_17_PENALTY=3.076045
PRIMER_LEFT_17_PENALTY=1.450652
PRIMER_RIGHT_17_PENALTY=1.625393
PRIMER_LEFT_17_SEQUENCE=TGTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_17_SEQUENCE=AAGATATTGCAAACGCTCTGG
PRIMER_LEFT_17=911,21
PRIMER_RIGHT_17=1811,21
PRIMER_LEFT_17_TM=60.451
PRIMER_RIGHT_17_TM=59.375
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=901
PRIMER_PAIR_18_PENALTY=3.100886
PRIMER_LEFT_18_PENALTY=2.931414
PRIMER_RIGHT_18_PENALTY=0.169471
PRIMER_LEFT_18_SEQUENCE=AAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_18_SEQUENCE=CTGCCTCAAGACCAACATCA
PRIMER_LEFT_18=916,20
PRIMER_RIGHT_18=1911,20
PRIMER_LEFT_18_TM=57.069
PRIMER_RIGHT_18_TM=59.831
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=996
PRIMER_PAIR_19_PENALTY=3.116946
PRIMER_LEFT_19_PENALTY=1.738092
PRIMER_RIGHT_19_PENALTY=1.378854
PRIMER_LEFT_19_SEQUENCE=GCTGTCAAAACACTGTCCCTG
PRIMER_RIGHT_19_SEQUENCE=CAACACTTTACTCCGGTCCA
PRIMER_LEFT_19=909,21
PRIMER_RIGHT_19=1838,20
PRIMER_LEFT_19_TM=60.738
PRIMER_RIGHT_19_TM=58.621
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=930
PRIMER_PAIR_20_PENALTY=3.132212
PRIMER_LEFT_20_PENALTY=1.753359
PRIMER_RIGHT_20_PENALTY=1.378854
PRIMER_LEFT_20_SEQUENCE=AAGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_20_SEQUENCE=CAACACTTTACTCCGGTCCA
PRIMER_LEFT_20=907,21
PRIMER_RIGHT_20=1838,20
PRIMER_LEFT_20_TM=59.247
PRIMER_RIGHT_20_TM=58.621
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.1000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=932
PRIMER_PAIR_21_PENALTY=3.226481
PRIMER_LEFT_21_PENALTY=2.142370
PRIMER_RIGHT_21_PENALTY=1.084110
PRIMER_LEFT_21_SEQUENCE=AGCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_21_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_21=908,21
PRIMER_RIGHT_21=1835,20
PRIMER_LEFT_21_TM=58.858
PRIMER_RIGHT_21_TM=61.084
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=928
PRIMER_PAIR_22_PENALTY=3.272983
PRIMER_LEFT_22_PENALTY=1.738092
PRIMER_RIGHT_22_PENALTY=1.534890
PRIMER_LEFT_22_SEQUENCE=GCTGTCAAAACACTGTCCCTG
PRIMER_RIGHT_22_SEQUENCE=TCAACACTTTACTCCGGTCCA
PRIMER_LEFT_22=909,21
PRIMER_RIGHT_22=1839,21
PRIMER_LEFT_22_TM=60.738
PRIMER_RIGHT_22_TM=60.535
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=931
PRIMER_PAIR_23_PENALTY=3.288249
PRIMER_LEFT_23_PENALTY=1.753359
PRIMER_RIGHT_23_PENALTY=1.534890
PRIMER_LEFT_23_SEQUENCE=AAGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_23_SEQUENCE=TCAACACTTTACTCCGGTCCA
PRIMER_LEFT_23=907,21
PRIMER_RIGHT_23=1839,21
PRIMER_LEFT_23_TM=59.247
PRIMER_RIGHT_23_TM=60.535
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.1000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=933
PRIMER_PAIR_24_PENALTY=3.293453
PRIMER_LEFT_24_PENALTY=2.209343
PRIMER_RIGHT_24_PENALTY=1.084110
PRIMER_LEFT_24_SEQUENCE=AAGCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_24_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_24=907,22
PRIMER_RIGHT_24=1835,20
PRIMER_LEFT_24_TM=60.209
PRIMER_RIGHT_24_TM=61.084
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=929
PRIMER_PAIR_25_PENALTY=3.314398
PRIMER_LEFT_25_PENALTY=2.230287
PRIMER_RIGHT_25_PENALTY=1.084110
PRIMER_LEFT_25_SEQUENCE=GCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_25_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_25=909,20
PRIMER_RIGHT_25=1835,20
PRIMER_LEFT_25_TM=57.770
PRIMER_RIGHT_25_TM=61.084
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=927
PRIMER_PAIR_26_PENALTY=3.314398
PRIMER_LEFT_26_PENALTY=2.230287
PRIMER_RIGHT_26_PENALTY=1.084110
PRIMER_LEFT_26_SEQUENCE=AGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_26_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_26=908,20
PRIMER_RIGHT_26=1835,20
PRIMER_LEFT_26_TM=57.770
PRIMER_RIGHT_26_TM=61.084
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.1000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=928
PRIMER_PAIR_27_PENALTY=3.339355
PRIMER_LEFT_27_PENALTY=2.931414
PRIMER_RIGHT_27_PENALTY=0.407941
PRIMER_LEFT_27_SEQUENCE=AAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_27_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_27=916,20
PRIMER_RIGHT_27=1834,20
PRIMER_LEFT_27_TM=57.069
PRIMER_RIGHT_27_TM=59.592
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.2000
PRIMER_RIGHT_27_END_STABILITY=8.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=919
PRIMER_PAIR_28_PENALTY=3.363486
PRIMER_LEFT_28_PENALTY=1.738092
PRIMER_RIGHT_28_PENALTY=1.625393
PRIMER_LEFT_28_SEQUENCE=GCTGTCAAAACACTGTCCCTG
PRIMER_RIGHT_28_SEQUENCE=AAGATATTGCAAACGCTCTGG
PRIMER_LEFT_28=909,21
PRIMER_RIGHT_28=1811,21
PRIMER_LEFT_28_TM=60.738
PRIMER_RIGHT_28_TM=59.375
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=903
PRIMER_PAIR_29_PENALTY=3.378752
PRIMER_LEFT_29_PENALTY=1.753359
PRIMER_RIGHT_29_PENALTY=1.625393
PRIMER_LEFT_29_SEQUENCE=AAGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_29_SEQUENCE=AAGATATTGCAAACGCTCTGG
PRIMER_LEFT_29=907,21
PRIMER_RIGHT_29=1811,21
PRIMER_LEFT_29_TM=59.247
PRIMER_RIGHT_29_TM=59.375
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.1000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=905
PRIMER_PAIR_30_PENALTY=3.451957
PRIMER_LEFT_30_PENALTY=1.450652
PRIMER_RIGHT_30_PENALTY=2.001306
PRIMER_LEFT_30_SEQUENCE=TGTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_30_SEQUENCE=AGATATTGCAAACGCTCTGG
PRIMER_LEFT_30=911,21
PRIMER_RIGHT_30=1810,20
PRIMER_LEFT_30_TM=60.451
PRIMER_RIGHT_30_TM=57.999
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=900
PRIMER_PAIR_31_PENALTY=3.452082
PRIMER_LEFT_31_PENALTY=3.282611
PRIMER_RIGHT_31_PENALTY=0.169471
PRIMER_LEFT_31_SEQUENCE=TCAAAACACTGTCCCTGGTAG
PRIMER_RIGHT_31_SEQUENCE=CTGCCTCAAGACCAACATCA
PRIMER_LEFT_31=913,21
PRIMER_RIGHT_31=1911,20
PRIMER_LEFT_31_TM=57.717
PRIMER_RIGHT_31_TM=59.831
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=999
PRIMER_PAIR_32_PENALTY=3.477123
PRIMER_LEFT_32_PENALTY=3.307651
PRIMER_RIGHT_32_PENALTY=0.169471
PRIMER_LEFT_32_SEQUENCE=CAAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_32_SEQUENCE=CTGCCTCAAGACCAACATCA
PRIMER_LEFT_32=914,22
PRIMER_RIGHT_32=1911,20
PRIMER_LEFT_32_TM=61.308
PRIMER_RIGHT_32_TM=59.831
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.2000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=998
PRIMER_PAIR_33_PENALTY=3.493277
PRIMER_LEFT_33_PENALTY=3.085335
PRIMER_RIGHT_33_PENALTY=0.407941
PRIMER_LEFT_33_SEQUENCE=GAAGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_33_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_33=906,22
PRIMER_RIGHT_33=1834,20
PRIMER_LEFT_33_TM=61.085
PRIMER_RIGHT_33_TM=59.592
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.1000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=929
PRIMER_PAIR_34_PENALTY=3.516630
PRIMER_LEFT_34_PENALTY=2.931414
PRIMER_RIGHT_34_PENALTY=0.585216
PRIMER_LEFT_34_SEQUENCE=AAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_34_SEQUENCE=TTTCTGCCTCAAGACCAACA
PRIMER_LEFT_34=916,20
PRIMER_RIGHT_34=1914,20
PRIMER_LEFT_34_TM=57.069
PRIMER_RIGHT_34_TM=59.415
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.2000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=999
PRIMER_PAIR_35_PENALTY=3.521224
PRIMER_LEFT_35_PENALTY=2.142370
PRIMER_RIGHT_35_PENALTY=1.378854
PRIMER_LEFT_35_SEQUENCE=AGCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_35_SEQUENCE=CAACACTTTACTCCGGTCCA
PRIMER_LEFT_35=908,21
PRIMER_RIGHT_35=1838,20
PRIMER_LEFT_35_TM=58.858
PRIMER_RIGHT_35_TM=58.621
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=931
PRIMER_PAIR_36_PENALTY=3.522154
PRIMER_LEFT_36_PENALTY=2.438044
PRIMER_RIGHT_36_PENALTY=1.084110
PRIMER_LEFT_36_SEQUENCE=AAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_36_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_36=915,21
PRIMER_RIGHT_36=1835,20
PRIMER_LEFT_36_TM=58.562
PRIMER_RIGHT_36_TM=61.084
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.2000
PRIMER_RIGHT_36_END_STABILITY=8.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=921
PRIMER_PAIR_37_PENALTY=3.544135
PRIMER_LEFT_37_PENALTY=3.374664
PRIMER_RIGHT_37_PENALTY=0.169471
PRIMER_LEFT_37_SEQUENCE=GTCAAAACACTGTCCCTGGTAG
PRIMER_RIGHT_37_SEQUENCE=CTGCCTCAAGACCAACATCA
PRIMER_LEFT_37=912,22
PRIMER_RIGHT_37=1911,20
PRIMER_LEFT_37_TM=58.625
PRIMER_RIGHT_37_TM=59.831
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=1000
PRIMER_PAIR_38_PENALTY=3.562282
PRIMER_LEFT_38_PENALTY=3.154341
PRIMER_RIGHT_38_PENALTY=0.407941
PRIMER_LEFT_38_SEQUENCE=AAGAAGCTGTCAAAACACTGTCC
PRIMER_RIGHT_38_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_38=904,23
PRIMER_RIGHT_38=1834,20
PRIMER_LEFT_38_TM=59.846
PRIMER_RIGHT_38_TM=59.592
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=931
PRIMER_PAIR_39_PENALTY=3.588197
PRIMER_LEFT_39_PENALTY=2.209343
PRIMER_RIGHT_39_PENALTY=1.378854
PRIMER_LEFT_39_SEQUENCE=AAGCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_39_SEQUENCE=CAACACTTTACTCCGGTCCA
PRIMER_LEFT_39=907,22
PRIMER_RIGHT_39=1838,20
PRIMER_LEFT_39_TM=60.209
PRIMER_RIGHT_39_TM=58.621
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=932
PRIMER_PAIR_40_PENALTY=3.609141
PRIMER_LEFT_40_PENALTY=2.230287
PRIMER_RIGHT_40_PENALTY=1.378854
PRIMER_LEFT_40_SEQUENCE=GCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_40_SEQUENCE=CAACACTTTACTCCGGTCCA
PRIMER_LEFT_40=909,20
PRIMER_RIGHT_40=1838,20
PRIMER_LEFT_40_TM=57.770
PRIMER_RIGHT_40_TM=58.621
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=930
PRIMER_PAIR_41_PENALTY=3.609141
PRIMER_LEFT_41_PENALTY=2.230287
PRIMER_RIGHT_41_PENALTY=1.378854
PRIMER_LEFT_41_SEQUENCE=AGCTGTCAAAACACTGTCCC
PRIMER_RIGHT_41_SEQUENCE=CAACACTTTACTCCGGTCCA
PRIMER_LEFT_41=908,20
PRIMER_RIGHT_41=1838,20
PRIMER_LEFT_41_TM=57.770
PRIMER_RIGHT_41_TM=58.621
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.1000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=931
PRIMER_PAIR_42_PENALTY=3.635889
PRIMER_LEFT_42_PENALTY=2.438044
PRIMER_RIGHT_42_PENALTY=1.197845
PRIMER_LEFT_42_SEQUENCE=AAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_42_SEQUENCE=TCTGCCTCAAGACCAACATC
PRIMER_LEFT_42=915,21
PRIMER_RIGHT_42=1912,20
PRIMER_LEFT_42_TM=58.562
PRIMER_RIGHT_42_TM=58.802
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.2000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=998
PRIMER_PAIR_43_PENALTY=3.637339
PRIMER_LEFT_43_PENALTY=2.553229
PRIMER_RIGHT_43_PENALTY=1.084110
PRIMER_LEFT_43_SEQUENCE=GTCAAAACACTGTCCCTGGT
PRIMER_RIGHT_43_SEQUENCE=CACTTTACTCCGGTCCACGA
PRIMER_LEFT_43=912,20
PRIMER_RIGHT_43=1835,20
PRIMER_LEFT_43_TM=57.447
PRIMER_RIGHT_43_TM=61.084
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=8.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=924
PRIMER_PAIR_44_PENALTY=3.677261
PRIMER_LEFT_44_PENALTY=2.142370
PRIMER_RIGHT_44_PENALTY=1.534890
PRIMER_LEFT_44_SEQUENCE=AGCTGTCAAAACACTGTCCCT
PRIMER_RIGHT_44_SEQUENCE=TCAACACTTTACTCCGGTCCA
PRIMER_LEFT_44=908,21
PRIMER_RIGHT_44=1839,21
PRIMER_LEFT_44_TM=58.858
PRIMER_RIGHT_44_TM=60.535
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=932
PRIMER_PAIR_45_PENALTY=3.685610
PRIMER_LEFT_45_PENALTY=2.438044
PRIMER_RIGHT_45_PENALTY=1.247566
PRIMER_LEFT_45_SEQUENCE=AAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_45_SEQUENCE=TTCTGCCTCAAGACCAACATC
PRIMER_LEFT_45=915,21
PRIMER_RIGHT_45=1913,21
PRIMER_LEFT_45_TM=58.562
PRIMER_RIGHT_45_TM=60.248
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.2000
PRIMER_RIGHT_45_END_STABILITY=6.3000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=999
PRIMER_PAIR_46_PENALTY=3.690552
PRIMER_LEFT_46_PENALTY=3.282611
PRIMER_RIGHT_46_PENALTY=0.407941
PRIMER_LEFT_46_SEQUENCE=TCAAAACACTGTCCCTGGTAG
PRIMER_RIGHT_46_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_46=913,21
PRIMER_RIGHT_46=1834,20
PRIMER_LEFT_46_TM=57.717
PRIMER_RIGHT_46_TM=59.592
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=922
PRIMER_PAIR_47_PENALTY=3.715592
PRIMER_LEFT_47_PENALTY=3.307651
PRIMER_RIGHT_47_PENALTY=0.407941
PRIMER_LEFT_47_SEQUENCE=CAAAACACTGTCCCTGGTAGGA
PRIMER_RIGHT_47_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_47=914,22
PRIMER_RIGHT_47=1834,20
PRIMER_LEFT_47_TM=61.308
PRIMER_RIGHT_47_TM=59.592
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.2000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=921
PRIMER_PAIR_48_PENALTY=3.739398
PRIMER_LEFT_48_PENALTY=1.738092
PRIMER_RIGHT_48_PENALTY=2.001306
PRIMER_LEFT_48_SEQUENCE=GCTGTCAAAACACTGTCCCTG
PRIMER_RIGHT_48_SEQUENCE=AGATATTGCAAACGCTCTGG
PRIMER_LEFT_48=909,21
PRIMER_RIGHT_48=1810,20
PRIMER_LEFT_48_TM=60.738
PRIMER_RIGHT_48_TM=57.999
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=902
PRIMER_PAIR_49_PENALTY=3.739452
PRIMER_LEFT_49_PENALTY=3.331511
PRIMER_RIGHT_49_PENALTY=0.407941
PRIMER_LEFT_49_SEQUENCE=CTGTCAAAACACTGTCCCTG
PRIMER_RIGHT_49_SEQUENCE=ACTTTACTCCGGTCCACGAA
PRIMER_LEFT_49=910,20
PRIMER_RIGHT_49=1834,20
PRIMER_LEFT_49_TM=56.668
PRIMER_RIGHT_49_TM=59.592
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=925
=
SEQUENCE_ID=MAGIC.149_4_5539924_5540291_edi-0_wil-2_114320_161784_TRUE
SEQUENCE_TARGET=950,467
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_14380885_14381052_tsu-0_can-0_40890_7075_TRUE
SEQUENCE_TARGET=950,267
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_14044843_14045483_zu-0_ct-1_5861_105612_TRUE
SEQUENCE_TARGET=950,740
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_10635169_10635194_hi-0_ler-0_6816_177099_TRUE
SEQUENCE_TARGET=950,125
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNGCTCTGAAGACNGCTTCCAATATNNGNNNCNTTGNNNATNCTGNNANCNGGTNCCNGAAATAGCGATACTGTTCTTAAATTGCATAGATTCATCTTCTAGAATCTAGAGGCCACGGTATATTTTTGAACATTTGTTTCCAGCTACCTCTTGTTCAATTTGTTTTAAACTTGTATATTCGTGCAATCTGCAGCTCGGAGCCTGATTTTTTAGATNNANNCNTNANGNANACTTTCNGGATGCTGAATGANTTGTCTCCATGNGNNNNNNNNNNNNNNNNNNNNNNNATTGAATCNCGTTCNAAGGAACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATTATTNNATGNAGAAAATNTNGATGTNAGAGCGCANGGAAGANNGNAANANTTTCTAAGGAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNAAGGAAGNAGGATTGTTATAANAACNAGAGACAAGANACTGCTGCAGAANAANGCNGATGCNACNTANGTNGTTCCNAGATTAAATGACAGGGAAGCTATGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNAGAATTNNTNGATCTANCAAACNANTTTGTNTNNTANGCNAANGGNCNNCCNTTAGCTTTGAAGTTGTTAGGTNNNGGTCTANNNNNNNANGANANNANNTACTGGNNGNNNAAATNGGAGNNNTTANNGGTAANNCCAGACAAGGAGNTNCANANAGNGCTNAAANNNAGNTATNAAGNACTNGANGATGANCAGAAGNGCNTATTTCTNGACATNGCATGTTTTTTCAGNNCAGANAAAGCAGATTTNGTNTCNANCATNNNNNNNTCAGACNNNNNNNNNNNNNNNNNNNNNATGANNGANCTNGANGANAAGTGCNTNGTAACNNNNNNNNNNNNNNNNNTTGAGATGCATGATCTANTGCATNCAATGGGGAANGAAATTGGANNNNNNNNATCCATCANAANGGCAGGCNAACGTNNNAGGTTNTGGAACCACAAAGATATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNNTNCNACCAGGGNACTGAATNTGTTAGAGGCATCTTCTTNAACNTGTNNGANGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCNACANTTCTCANTGTTCNCAGTNGTGTGATAANGACCATANATTTCAGTNCTNNNAAGNNNNNNNNNNNNNNNNNNNNGAGCTNGTNTACCTTCACTGGCANNNNNNNNNNNNNGANTNNCTNCCATCAGANTTCNANCCNNAGGAACTTGTNGATCTTANTCTNCGTTATAGNNACATCAAACAACTGTGGGAAGATGAGAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNCNGAATACAGAAANTTTAAGATGGGTCGANCTNNGTCANTCAANANACTTGCNNANTTTATCAGGNTTNTCCAGGGCNAAAANTCTTGAAAGATTGGATCTNGAAGGCTGTACGAGTTTGNNNNNNNNNNNNNNNNNNNNNCNNATGAACNANCTTATTTACCTNAACCTCNGAGANTGCACA
PRIMER_LEFT_NUM_RETURNED=25
PRIMER_RIGHT_NUM_RETURNED=25
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=25
PRIMER_PAIR_0_PENALTY=5.099564
PRIMER_LEFT_0_PENALTY=2.341032
PRIMER_RIGHT_0_PENALTY=2.758532
PRIMER_LEFT_0_SEQUENCE=AAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_0_SEQUENCE=CCTTCTCATCTTCCCACAGTT
PRIMER_LEFT_0=814,21
PRIMER_RIGHT_0=1753,21
PRIMER_LEFT_0_TM=58.659
PRIMER_RIGHT_0_TM=58.241
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=940
PRIMER_PAIR_1_PENALTY=5.554080
PRIMER_LEFT_1_PENALTY=2.795548
PRIMER_RIGHT_1_PENALTY=2.758532
PRIMER_LEFT_1_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_1_SEQUENCE=CCTTCTCATCTTCCCACAGTT
PRIMER_LEFT_1=815,20
PRIMER_RIGHT_1=1753,21
PRIMER_LEFT_1_TM=57.204
PRIMER_RIGHT_1_TM=58.241
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=939
PRIMER_PAIR_2_PENALTY=5.645708
PRIMER_LEFT_2_PENALTY=2.341032
PRIMER_RIGHT_2_PENALTY=3.304676
PRIMER_LEFT_2_SEQUENCE=AAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_2_SEQUENCE=TCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_2=814,21
PRIMER_RIGHT_2=1750,22
PRIMER_LEFT_2_TM=58.659
PRIMER_RIGHT_2_TM=58.695
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=937
PRIMER_PAIR_3_PENALTY=5.780795
PRIMER_LEFT_3_PENALTY=2.341032
PRIMER_RIGHT_3_PENALTY=3.439762
PRIMER_LEFT_3_SEQUENCE=AAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_3_SEQUENCE=CTTCCCACAGTTGTTTGATGT
PRIMER_LEFT_3=814,21
PRIMER_RIGHT_3=1744,21
PRIMER_LEFT_3_TM=58.659
PRIMER_RIGHT_3_TM=57.560
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=931
PRIMER_PAIR_4_PENALTY=5.956233
PRIMER_LEFT_4_PENALTY=2.341032
PRIMER_RIGHT_4_PENALTY=3.615201
PRIMER_LEFT_4_SEQUENCE=AAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_4_SEQUENCE=TTCCCACAGTTGTTTGATGT
PRIMER_LEFT_4=814,21
PRIMER_RIGHT_4=1743,20
PRIMER_LEFT_4_TM=58.659
PRIMER_RIGHT_4_TM=56.385
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=930
PRIMER_PAIR_5_PENALTY=6.040680
PRIMER_LEFT_5_PENALTY=3.282148
PRIMER_RIGHT_5_PENALTY=2.758532
PRIMER_LEFT_5_SEQUENCE=TAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_5_SEQUENCE=CCTTCTCATCTTCCCACAGTT
PRIMER_LEFT_5=813,22
PRIMER_RIGHT_5=1753,21
PRIMER_LEFT_5_TM=58.718
PRIMER_RIGHT_5_TM=58.241
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=941
PRIMER_PAIR_6_PENALTY=6.100224
PRIMER_LEFT_6_PENALTY=2.795548
PRIMER_RIGHT_6_PENALTY=3.304676
PRIMER_LEFT_6_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_6_SEQUENCE=TCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_6=815,20
PRIMER_RIGHT_6=1750,22
PRIMER_LEFT_6_TM=57.204
PRIMER_RIGHT_6_TM=58.695
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=936
PRIMER_PAIR_7_PENALTY=6.235311
PRIMER_LEFT_7_PENALTY=2.795548
PRIMER_RIGHT_7_PENALTY=3.439762
PRIMER_LEFT_7_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_7_SEQUENCE=CTTCCCACAGTTGTTTGATGT
PRIMER_LEFT_7=815,20
PRIMER_RIGHT_7=1744,21
PRIMER_LEFT_7_TM=57.204
PRIMER_RIGHT_7_TM=57.560
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=6.3000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=930
PRIMER_PAIR_8_PENALTY=6.410750
PRIMER_LEFT_8_PENALTY=2.795548
PRIMER_RIGHT_8_PENALTY=3.615201
PRIMER_LEFT_8_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_8_SEQUENCE=TTCCCACAGTTGTTTGATGT
PRIMER_LEFT_8=815,20
PRIMER_RIGHT_8=1743,20
PRIMER_LEFT_8_TM=57.204
PRIMER_RIGHT_8_TM=56.385
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=6.3000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=929
PRIMER_PAIR_9_PENALTY=6.586824
PRIMER_LEFT_9_PENALTY=3.282148
PRIMER_RIGHT_9_PENALTY=3.304676
PRIMER_LEFT_9_SEQUENCE=TAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_9_SEQUENCE=TCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_9=813,22
PRIMER_RIGHT_9=1750,22
PRIMER_LEFT_9_TM=58.718
PRIMER_RIGHT_9_TM=58.695
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=938
PRIMER_PAIR_10_PENALTY=6.721911
PRIMER_LEFT_10_PENALTY=3.282148
PRIMER_RIGHT_10_PENALTY=3.439762
PRIMER_LEFT_10_SEQUENCE=TAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_10_SEQUENCE=CTTCCCACAGTTGTTTGATGT
PRIMER_LEFT_10=813,22
PRIMER_RIGHT_10=1744,21
PRIMER_LEFT_10_TM=58.718
PRIMER_RIGHT_10_TM=57.560
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=932
PRIMER_PAIR_11_PENALTY=6.897349
PRIMER_LEFT_11_PENALTY=3.282148
PRIMER_RIGHT_11_PENALTY=3.615201
PRIMER_LEFT_11_SEQUENCE=TAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_11_SEQUENCE=TTCCCACAGTTGTTTGATGT
PRIMER_LEFT_11=813,22
PRIMER_RIGHT_11=1743,20
PRIMER_LEFT_11_TM=58.718
PRIMER_RIGHT_11_TM=56.385
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.3000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=931
PRIMER_PAIR_12_PENALTY=7.043033
PRIMER_LEFT_12_PENALTY=2.795548
PRIMER_RIGHT_12_PENALTY=4.247485
PRIMER_LEFT_12_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_12_SEQUENCE=CTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_12=815,20
PRIMER_RIGHT_12=1749,21
PRIMER_LEFT_12_TM=57.204
PRIMER_RIGHT_12_TM=56.753
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=935
PRIMER_PAIR_13_PENALTY=7.234934
PRIMER_LEFT_13_PENALTY=2.341032
PRIMER_RIGHT_13_PENALTY=4.893902
PRIMER_LEFT_13_SEQUENCE=AAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_13_SEQUENCE=CTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_13=814,21
PRIMER_RIGHT_13=1752,24
PRIMER_LEFT_13_TM=58.659
PRIMER_RIGHT_13_TM=60.894
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=41.667
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=939
PRIMER_PAIR_14_PENALTY=7.248007
PRIMER_LEFT_14_PENALTY=2.795548
PRIMER_RIGHT_14_PENALTY=4.452459
PRIMER_LEFT_14_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_14_SEQUENCE=TCATCTTCCCACAGTTGTTT
PRIMER_LEFT_14=815,20
PRIMER_RIGHT_14=1748,20
PRIMER_LEFT_14_TM=57.204
PRIMER_RIGHT_14_TM=55.548
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=934
PRIMER_PAIR_15_PENALTY=7.689450
PRIMER_LEFT_15_PENALTY=2.795548
PRIMER_RIGHT_15_PENALTY=4.893902
PRIMER_LEFT_15_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_15_SEQUENCE=CTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_15=815,20
PRIMER_RIGHT_15=1752,24
PRIMER_LEFT_15_TM=57.204
PRIMER_RIGHT_15_TM=60.894
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=41.667
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=938
PRIMER_PAIR_16_PENALTY=8.176050
PRIMER_LEFT_16_PENALTY=3.282148
PRIMER_RIGHT_16_PENALTY=4.893902
PRIMER_LEFT_16_SEQUENCE=TAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_16_SEQUENCE=CTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_16=813,22
PRIMER_RIGHT_16=1752,24
PRIMER_LEFT_16_TM=58.718
PRIMER_RIGHT_16_TM=60.894
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=940
PRIMER_PAIR_17_PENALTY=9.505162
PRIMER_LEFT_17_PENALTY=6.746630
PRIMER_RIGHT_17_PENALTY=2.758532
PRIMER_LEFT_17_SEQUENCE=GATTAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_17_SEQUENCE=CCTTCTCATCTTCCCACAGTT
PRIMER_LEFT_17=810,25
PRIMER_RIGHT_17=1753,21
PRIMER_LEFT_17_TM=61.747
PRIMER_RIGHT_17_TM=58.241
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=944
PRIMER_PAIR_18_PENALTY=10.051306
PRIMER_LEFT_18_PENALTY=6.746630
PRIMER_RIGHT_18_PENALTY=3.304676
PRIMER_LEFT_18_SEQUENCE=GATTAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_18_SEQUENCE=TCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_18=810,25
PRIMER_RIGHT_18=1750,22
PRIMER_LEFT_18_TM=61.747
PRIMER_RIGHT_18_TM=58.695
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=941
PRIMER_PAIR_19_PENALTY=10.361831
PRIMER_LEFT_19_PENALTY=6.746630
PRIMER_RIGHT_19_PENALTY=3.615201
PRIMER_LEFT_19_SEQUENCE=GATTAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_19_SEQUENCE=TTCCCACAGTTGTTTGATGT
PRIMER_LEFT_19=810,25
PRIMER_RIGHT_19=1743,20
PRIMER_LEFT_19_TM=61.747
PRIMER_RIGHT_19_TM=56.385
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=934
PRIMER_PAIR_20_PENALTY=11.246421
PRIMER_LEFT_20_PENALTY=2.341032
PRIMER_RIGHT_20_PENALTY=8.905389
PRIMER_LEFT_20_SEQUENCE=AAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_20_SEQUENCE=CCTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_20=814,21
PRIMER_RIGHT_20=1753,25
PRIMER_LEFT_20_TM=58.659
PRIMER_RIGHT_20_TM=63.905
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=44.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=940
PRIMER_PAIR_21_PENALTY=11.640532
PRIMER_LEFT_21_PENALTY=6.746630
PRIMER_RIGHT_21_PENALTY=4.893902
PRIMER_LEFT_21_SEQUENCE=GATTAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_21_SEQUENCE=CTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_21=810,25
PRIMER_RIGHT_21=1752,24
PRIMER_LEFT_21_TM=61.747
PRIMER_RIGHT_21_TM=60.894
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=41.667
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=943
PRIMER_PAIR_22_PENALTY=11.700937
PRIMER_LEFT_22_PENALTY=2.795548
PRIMER_RIGHT_22_PENALTY=8.905389
PRIMER_LEFT_22_SEQUENCE=AATGACAGGGAAGCTATGGA
PRIMER_RIGHT_22_SEQUENCE=CCTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_22=815,20
PRIMER_RIGHT_22=1753,25
PRIMER_LEFT_22_TM=57.204
PRIMER_RIGHT_22_TM=63.905
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=44.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=939
PRIMER_PAIR_23_PENALTY=12.187537
PRIMER_LEFT_23_PENALTY=3.282148
PRIMER_RIGHT_23_PENALTY=8.905389
PRIMER_LEFT_23_SEQUENCE=TAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_23_SEQUENCE=CCTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_23=813,22
PRIMER_RIGHT_23=1753,25
PRIMER_LEFT_23_TM=58.718
PRIMER_RIGHT_23_TM=63.905
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=44.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=941
PRIMER_PAIR_24_PENALTY=15.652019
PRIMER_LEFT_24_PENALTY=6.746630
PRIMER_RIGHT_24_PENALTY=8.905389
PRIMER_LEFT_24_SEQUENCE=GATTAAATGACAGGGAAGCTATGGA
PRIMER_RIGHT_24_SEQUENCE=CCTTCTCATCTTCCCACAGTTGTTT
PRIMER_LEFT_24=810,25
PRIMER_RIGHT_24=1753,25
PRIMER_LEFT_24_TM=61.747
PRIMER_RIGHT_24_TM=63.905
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=44.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=944
=
SEQUENCE_ID=MAGIC.329_3_12725371_12725848_mt-0_wil-2_14266_3693_TRUE
SEQUENCE_TARGET=950,577
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_5286478_5287033_col-0_sf-2_8700_86159_TRUE
SEQUENCE_TARGET=950,655
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCCAAGGAAATAGTACTCTTTTTTNCCCAAACACGTGTGAGGTGGTTTAAAGGGTATAATTGTCAATTTCAAAATAAGAGGTTTATTTGGTGCGAATCTCAAACGTCCAAAATGTTTGAAATCATATAACCATGGGTTGTATTGTAACTATAGCTTACCATCCAAATCATAAAAACAATAAATGATGTGTACATTTTTCCAACAGCGATCATGCTTATTTCTATGAAAAATGATTTTGACATTATTTGAATTGATCTTTATACAAATAAATAAAGTTTTCTACTAGTTTAAAAAAAAANATAAATAAACAAATGAATTATGAATTTATGATTACGTCTCTCTATAATCATATTTGTTCNTTTTTAATCATTGAAAGCTNAACTTATGGGGAATGAATNATCTGAAATGACAGCTTCCTAATTTTTACAGCTACGGTAATATAGGGTAATGGGTCAAGTAAATGTAACGTGTTAAAGATTAAAAAGTAAAAGACGTGCGTCAAAGAAACAGAGACGTCAAGATTAGTTTAAAACTTACTAATTACAGTAGTTAATTCGGAATTAAGCTAAGTCCAGAAAATTCCATGAAGCAGCATCCATCGTCAGCATCTTCAAGTCACANTTTTTCTTATTTTCGCAAAANCCTTCAAAAACCCGTTCCGGTCTGTTTTCTCCTCTCGAAACCCTCGATCGTTGCCGCGAAACAGCTCCGGTGACCTAAAATCGTTCGACGAAACGGTTTCTCTTCCTCCTTGTCGTTTTCACTTTTCACGTCGTTCATTGCATCTATAGGTCTTCGTTTGTTCTGCATTGTACGGATTCTCTTCCGATCGACGATTCAGCTTCCGGTATAGCTTCTCCTCTCTTCCATGGTCTTTAAATCGTCGTTTCTGTTTGATTTTTAGTAACGTTGAGCTGCTTTTGTTTTATCGGAAGCAACGTCCGTACAGGTTTGCATTTTTGTAGCTTAACGGTGAGTGTTTTCCGGTATATTTGTTGGATTTTCTGATCAACGTTTGCTAAAAAACACCGTGTTTTATACAGATTATCTTTGGTATTTCGAATTAACTGTTACATCTGTTGCTTTTGTGTGGAGCTTATGAACAGTAAGCGNTTATTTTCANGTATTTGAGTCTCTGAAGTTTAGGTGGCTTGATTTTGTGGGTAAAGTTTCATATACAGTGATGACATTTTGTTGTTGCTTCGTAAAATGGCTTTGTTATACAGTAATGACTTCAATTTCTGACAAGTTTATACTTGTGAATGGTGGCAACTGTTTAGGTTAGTAGATGAAATGTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9445779_9446101_wil-2_po-0_1202_23319_TRUE
SEQUENCE_TARGET=950,422
SEQUENCE_TEMPLATE=TATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGTTGAGTGNAATCCTTTCACAAGNTTTTCTTAAGAAANTGAAAAACAATCATANATGTACAATACTAGAGATATATNATGAGTCATGANTCATATTAATCGCGGTTTTTGTAATATCATAAAGCTTTCTACTTTAGGCTTGNCAGTGATTNAATACAGTAACATATCACATAAATATGAGAAATGGAGGCTCTTTAATTAGGCCTTTACAATATACATATATATTAGAAATAGAAAGAAAAGNAAAAANTCGNCCGACAACAAGATTGGTGAAGAAACGCNATAATTGAACCATTAAGTACAGTCTAATACCCGCAGAAAATATNACACCACCTACTCTTAGTGGCAATGCTGTACTTTAACATNTTGTCGAGACAAGGAGNCCAGGTTNCGTGAAATCATGNAGATGANGNNGCATAAACAATCACCAAAGTTCACCCTATGAATCCAANCCCTTCTAGCTTAGTGGCTATGATCATAGCCGACATCTCATANAGTCANANTCCATTTCGGAGTAAATNATGCGNTGAACAANANTNAGCTTGTTCNCATTNCAANATANCAAATTGGATACTATATNNNAAAAANNNNNGANTGTNANNNNTTNGATCAAATCTAACTNTTAGAGANNNATNATANAANAAACATACATGATTTNAAGTAGTTTACTATAATTTNNATANANTTAGAATTTGGAAANAGNGAACGATTATAGATTNTGNTAGTAGANTTTGGAAANAGAAATGCTNTNAAANAACNNNNNNNNNNCTTNTTNTTTNANNTTTNATTNATNTAACAANCNNAAATATANTTTNNGATATTGNCCANNNNNNNNNCGGGCTACAAAACCGGAAATANACATTNCNGNTANAGCTTAATCCGTATTTTCNNTNTATTTCGCGTTNAATACTNTGTTTGTTTANTATTAAGATCACCATACTATATNACAAANAAACAAAAANNNTNNCACAACTACAATAAAATGCTAACAAGCCGAAAGTGTACNGCANCAGTGTGTAATNGAANAAGTATCATTANAGAAGGNCATGCTCGATTCATTANTCTATACCATTCCTTANTANNNNNNTATTACATGGGCAANAACGAAACAGGATAAANANGAGAGAGTGATCAGTGATGATGNATGGTCATGGAGTTTGCTGTTTTTAGGGAAGAACAAGAAGTATCGTATGATTGTCAAGACTTCTTCTGTCGGCGCGTGACCNGACAAATCNACACGCGCGTGTGTGTGAGTCACGTGGTTCGACGTCTCTCANTGGCTNATNNTATCATNTTGGGTCCACGTGAGTTTCTTCTTTACCTTTTTTTNTTNTNTTTTTNTTCTCTCCNNTTNTTTTNNAAGAACCCAAANNANCAGTATATTNCTACTTGANATCTTATGTTTTATATTTATAAANTTTTACCCTTNGNCANAAAATGTNTCNATAATTTTCTGCATCCTCACGTGAAGTCCACNTCTNTNNAAAANANNTATAGANAAAAGNTTTTAAAGTAAATTTNNNNNNNNNNATAAGATCTACNAATTGTGAAGNTTTATAGAATTAAATCTCAAAANTAATTTATAATCNTTTNGATAATNTATANTTNATTTANTAGAGTTACATNGGAGTTTNNTTCNANNNNNATATAAAACTTNTNANAATATTTNTANATAAATTTTNCANTTTNAAACTANCANCTTNNTAATAATAAANTATCNNAAGATACANCTTTTCCTAATAATTTAACAAAACAAAGTTTACTCAAATCNCAACTACACACGTTNTNNAATTTNACATTGCGTATNAANTTNGTCGANTCGTCAACTAATTCTAGAGTAAACAANATCANTGGCNANACTCTNAAGGAAAAANNGATNCATTTGATTATGTTACAAGTGATNAGAGTTTNGAANTCGTTTTGTGTCAAATGTTNCGGTCTCCNATATGTAAAAGTGGAAGGNTTNTCATGCTTCTNNTTNGTTTGTTCCAANNNNNNNNNNNNTATNGCCATGGAAAACATCNACCATATNGTCAAAATGACCNGTTAAATAANNAANNATTGTAANAACTATTNTTAAGTTNTCCTTNCNTTTCATGGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANNAAAAAAANTCAATCTGATTTTNAACNTTTNAAAGNATAATTNGGTTGANNTTTNTTTTNTTTTATTCTGATTNNTNGAAACTGGAAAGTAAGATGAATTNGACATNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_20837428_20837659_ler-0_rsch-4_132141_551398_TRUE
SEQUENCE_TARGET=950,331
SEQUENCE_TEMPLATE=CTTGCAGCTCATTTTACTATGATAATTTGTTTGTCAAATGGTATTGAATCTTGTGATTACCTGACATATATGGCACATCCTCCAACCGCGCGAGCTGCAGCATGATATTCAGCCATCGGATGCAAACTCTGCAATGAGAAACAGATAGATTCATTCACAANTTCAGAAAAGTTTGTTAAAGCAATTCAACGTACATGAAACATGTCCCAGTCCGGCTGCATAAACTCTCCAAGGAAAAGTGTGTTATATGCAACAGAAGCAATGTGGATCGTGTGTGAAGCGGGGTCTCTAGGCCAGAAATCGTCTGATGCTCTTATAACAGCAGTCTTTTTTGCNCTANAGTATCATTACAAGAAGAAATNAGAAGGGANANNNNNNNNNNNNNNNNNNNNNNNNTGAGAANAACGTTGNAGNTCNAGANGCTAACCTGTAGAGACCATCTGTGTTATGACTCATGCAAGAGATAATACCATTGTCAGGGAAGTTTCTTGAAATAGAAGCTTCCAAAGCCTGGTGATACTTCTTTGCAAGTTTGACACGACCACCATGTCCAGCTCCAAGAGTTTCNAGAATGTTTTGGACGTCTACTTTGACACCATCGANCCCAANGGATGCAAGGTANGAGTGAAGNTCNTTATAGAAGCTAAAGACTTTCTCAGGATTCACCAAACCAAGTCCATTCTTAGTTATNCTTTCTANNCATCCACAATTCTCANTGGACATAACTCCTGGTGAAGAAACTGGATACGCNACCTTNGATTCGTAGTGNTCCATACCAGAAACACCTGGTTTGACTCCTCCCCAATATCCNGTTATCGCATGCCAAACATAAACATACTTGAGNGAATTATTACTCTTAATGTCTGTGATAACATGTCCAAGACTCAATGNAGGGTCATCCACCCGGTGGCCTTCTTTGCCATCCTTTTGAAACTTNTGATTNTCCTTGATGTGAGTTAACCGGTTTGCNAAACTGAAGGNAANCANAAAAGATAAAGAGNGGTTTATTNNNNNNNNNNNNNNAACNNNNNNNNNNNNNNNNNNNNNNNNATTACTTACTTGGCAGCATTATCAGCGTTNAATTCTACACTGGTCTCATCCATGCCAACAGATTGCCATCCATCATCAATTATGACAAACTTTGGNGTAACCCCACCAGCTTTGAGGCTANAGAGAATCANTGCAGGTTTGGTTAAGTCACAGTTANTAAAGCCATCTTNAAAAGTAAAGANTCAGGAGNACCTCTCAAGNCCTTGCTTGACGTCTTTGGCTGTGACATTGGTNTAGAATGCATCCCAAGTGCACCAACCAAACCANTTCANCATATCTGGCATCTGAACACAACCAAAACAGGGGAAATTTTCAGAGTTANTTTTGNNATGNNTATAANCAAAGATCANATAAAANGTCTCAATGTTTCCTTACTTTCTTTCTCTCTCGGTGTGAAAATGTTTGAAGATGTTGTTCAACAGCCCTGCATAATCATTGAAAGGCANAGATTCTTAACNCTAAANAANTTTGGACATAAAGAAGTCTTNAGTTGAGACTTGAGAAACNTTACATACTTGACAGCTTTGGTAATGACATCAAAAGGGTCGGAGCCANCAGCCACAAAAACCAAATGACTCCCTTCGAATTGGTCAACGGTTGGATCACCTTGACCATACANAAACCATATAAATCTCAGAGAGAATCTAACNNAANNNANNCAACTCAAATCTAGCTANCTGTGTCAACTTACCACTTTCAAGACAAATCTCTAGNTCATTAGCCTCGTTCCCTTGAAGAACAGCTCTAAAATCTCCTTCAAGTATAGGNAAGAATACAACGTACGAAGAAGATTGATCTCNTCCTCCTAAATCAGAACCTTGGTTTGCTTCTACGATTAGAAACTGAGTCTCACAAGGAATCTCTTTCCCATTAGTCCCCATTCTTTGAGTCATCCACCATAANTTAAANCGGAAGACANACATAAACCTTAGGTCTCTGCCAAGNAAAAAAAAAACATTTATACCCTAAGCCTCAAGTAATTCAACAAATTATGTGACATCTAACAACANAAGTGTAGGAAAGGGATTNTGAACAGTACTCGAGTTTGCCAAGGGAGAAGACACGGTGGCTTCCGGTTTGATCTGACGTGACGCCGATGAACGCGCCGTCGATTAAGGCGTTTCCCGAAGCTGGGGTAACTAAGACATTCTCCGGAACGCCGTGAAGAACACGGTGACCTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.480009
PRIMER_LEFT_0_PENALTY=0.260155
PRIMER_RIGHT_0_PENALTY=0.219854
PRIMER_LEFT_0_SEQUENCE=TTTGCAAGTTTGACACGACC
PRIMER_RIGHT_0_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_0=523,20
PRIMER_RIGHT_0=1484,20
PRIMER_LEFT_0_TM=59.740
PRIMER_RIGHT_0_TM=60.220
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=962
PRIMER_PAIR_1_PENALTY=0.480611
PRIMER_LEFT_1_PENALTY=0.260757
PRIMER_RIGHT_1_PENALTY=0.219854
PRIMER_LEFT_1_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_1_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_1=544,20
PRIMER_RIGHT_1=1484,20
PRIMER_LEFT_1_TM=60.261
PRIMER_RIGHT_1_TM=60.220
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=941
PRIMER_PAIR_2_PENALTY=0.507194
PRIMER_LEFT_2_PENALTY=0.260757
PRIMER_RIGHT_2_PENALTY=0.246438
PRIMER_LEFT_2_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_2_SEQUENCE=CCCCTGTTTTGGTTGTGTTC
PRIMER_LEFT_2=544,20
PRIMER_RIGHT_2=1355,20
PRIMER_LEFT_2_TM=60.261
PRIMER_RIGHT_2_TM=60.246
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=812
PRIMER_PAIR_3_PENALTY=0.507194
PRIMER_LEFT_3_PENALTY=0.260757
PRIMER_RIGHT_3_PENALTY=0.246438
PRIMER_LEFT_3_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_3_SEQUENCE=TCCCCTGTTTTGGTTGTGTT
PRIMER_LEFT_3=544,20
PRIMER_RIGHT_3=1356,20
PRIMER_LEFT_3_TM=60.261
PRIMER_RIGHT_3_TM=60.246
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=813
PRIMER_PAIR_4_PENALTY=0.507194
PRIMER_LEFT_4_PENALTY=0.260757
PRIMER_RIGHT_4_PENALTY=0.246438
PRIMER_LEFT_4_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_4_SEQUENCE=TTCCCCTGTTTTGGTTGTGT
PRIMER_LEFT_4=544,20
PRIMER_RIGHT_4=1357,20
PRIMER_LEFT_4_TM=60.261
PRIMER_RIGHT_4_TM=60.246
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=814
PRIMER_PAIR_5_PENALTY=0.526314
PRIMER_LEFT_5_PENALTY=0.320610
PRIMER_RIGHT_5_PENALTY=0.205704
PRIMER_LEFT_5_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_5_SEQUENCE=TCAAGGGAACGAGGCTAATG
PRIMER_LEFT_5=910,20
PRIMER_RIGHT_5=1784,20
PRIMER_LEFT_5_TM=59.679
PRIMER_RIGHT_5_TM=60.206
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=6.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=875
PRIMER_PAIR_6_PENALTY=0.540464
PRIMER_LEFT_6_PENALTY=0.320610
PRIMER_RIGHT_6_PENALTY=0.219854
PRIMER_LEFT_6_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_6_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_6=910,20
PRIMER_RIGHT_6=1484,20
PRIMER_LEFT_6_TM=59.679
PRIMER_RIGHT_6_TM=60.220
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=575
PRIMER_PAIR_7_PENALTY=0.544801
PRIMER_LEFT_7_PENALTY=0.320610
PRIMER_RIGHT_7_PENALTY=0.224191
PRIMER_LEFT_7_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_7_SEQUENCE=TGTTCTTCAAGGGAACGAGG
PRIMER_LEFT_7=910,20
PRIMER_RIGHT_7=1790,20
PRIMER_LEFT_7_TM=59.679
PRIMER_RIGHT_7_TM=60.224
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=9.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=881
PRIMER_PAIR_8_PENALTY=0.567047
PRIMER_LEFT_8_PENALTY=0.320610
PRIMER_RIGHT_8_PENALTY=0.246438
PRIMER_LEFT_8_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_8_SEQUENCE=CCCCTGTTTTGGTTGTGTTC
PRIMER_LEFT_8=910,20
PRIMER_RIGHT_8=1355,20
PRIMER_LEFT_8_TM=59.679
PRIMER_RIGHT_8_TM=60.246
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=446
PRIMER_PAIR_9_PENALTY=0.567047
PRIMER_LEFT_9_PENALTY=0.320610
PRIMER_RIGHT_9_PENALTY=0.246438
PRIMER_LEFT_9_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_9_SEQUENCE=TCCCCTGTTTTGGTTGTGTT
PRIMER_LEFT_9=910,20
PRIMER_RIGHT_9=1356,20
PRIMER_LEFT_9_TM=59.679
PRIMER_RIGHT_9_TM=60.246
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=447
PRIMER_PAIR_10_PENALTY=0.567047
PRIMER_LEFT_10_PENALTY=0.320610
PRIMER_RIGHT_10_PENALTY=0.246438
PRIMER_LEFT_10_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_10_SEQUENCE=TTCCCCTGTTTTGGTTGTGT
PRIMER_LEFT_10=910,20
PRIMER_RIGHT_10=1357,20
PRIMER_LEFT_10_TM=59.679
PRIMER_RIGHT_10_TM=60.246
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=448
PRIMER_PAIR_11_PENALTY=0.578691
PRIMER_LEFT_11_PENALTY=0.260757
PRIMER_RIGHT_11_PENALTY=0.317935
PRIMER_LEFT_11_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_11_SEQUENCE=GGTTGTGTTCAGATGCCAGA
PRIMER_LEFT_11=544,20
PRIMER_RIGHT_11=1345,20
PRIMER_LEFT_11_TM=60.261
PRIMER_RIGHT_11_TM=59.682
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=802
PRIMER_PAIR_12_PENALTY=0.638544
PRIMER_LEFT_12_PENALTY=0.320610
PRIMER_RIGHT_12_PENALTY=0.317935
PRIMER_LEFT_12_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_12_SEQUENCE=GGTTGTGTTCAGATGCCAGA
PRIMER_LEFT_12=910,20
PRIMER_RIGHT_12=1345,20
PRIMER_LEFT_12_TM=59.679
PRIMER_RIGHT_12_TM=59.682
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=436
PRIMER_PAIR_13_PENALTY=0.714178
PRIMER_LEFT_13_PENALTY=0.260757
PRIMER_RIGHT_13_PENALTY=0.453422
PRIMER_LEFT_13_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_13_SEQUENCE=TTTGGTTGTGTTCAGATGCC
PRIMER_LEFT_13=544,20
PRIMER_RIGHT_13=1348,20
PRIMER_LEFT_13_TM=60.261
PRIMER_RIGHT_13_TM=59.547
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=805
PRIMER_PAIR_14_PENALTY=0.759671
PRIMER_LEFT_14_PENALTY=0.320610
PRIMER_RIGHT_14_PENALTY=0.439061
PRIMER_LEFT_14_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_14_SEQUENCE=TCATTTGGTTTTTGTGGCTG
PRIMER_LEFT_14=910,20
PRIMER_RIGHT_14=1624,20
PRIMER_LEFT_14_TM=59.679
PRIMER_RIGHT_14_TM=59.561
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=715
PRIMER_PAIR_15_PENALTY=0.767786
PRIMER_LEFT_15_PENALTY=0.562082
PRIMER_RIGHT_15_PENALTY=0.205704
PRIMER_LEFT_15_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_15_SEQUENCE=TCAAGGGAACGAGGCTAATG
PRIMER_LEFT_15=909,20
PRIMER_RIGHT_15=1784,20
PRIMER_LEFT_15_TM=60.562
PRIMER_RIGHT_15_TM=60.206
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=6.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=876
PRIMER_PAIR_16_PENALTY=0.774031
PRIMER_LEFT_16_PENALTY=0.320610
PRIMER_RIGHT_16_PENALTY=0.453422
PRIMER_LEFT_16_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_16_SEQUENCE=TTTGGTTGTGTTCAGATGCC
PRIMER_LEFT_16=910,20
PRIMER_RIGHT_16=1348,20
PRIMER_LEFT_16_TM=59.679
PRIMER_RIGHT_16_TM=59.547
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=439
PRIMER_PAIR_17_PENALTY=0.781936
PRIMER_LEFT_17_PENALTY=0.562082
PRIMER_RIGHT_17_PENALTY=0.219854
PRIMER_LEFT_17_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_17_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_17=909,20
PRIMER_RIGHT_17=1484,20
PRIMER_LEFT_17_TM=60.562
PRIMER_RIGHT_17_TM=60.220
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=576
PRIMER_PAIR_18_PENALTY=0.786273
PRIMER_LEFT_18_PENALTY=0.562082
PRIMER_RIGHT_18_PENALTY=0.224191
PRIMER_LEFT_18_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_18_SEQUENCE=TGTTCTTCAAGGGAACGAGG
PRIMER_LEFT_18=909,20
PRIMER_RIGHT_18=1790,20
PRIMER_LEFT_18_TM=60.562
PRIMER_RIGHT_18_TM=60.224
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=882
PRIMER_PAIR_19_PENALTY=0.794304
PRIMER_LEFT_19_PENALTY=0.320610
PRIMER_RIGHT_19_PENALTY=0.473695
PRIMER_LEFT_19_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_19_SEQUENCE=TCGAAGGGAGTCATTTGGTT
PRIMER_LEFT_19=910,20
PRIMER_RIGHT_19=1634,20
PRIMER_LEFT_19_TM=59.679
PRIMER_RIGHT_19_TM=59.526
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=725
PRIMER_PAIR_20_PENALTY=0.794304
PRIMER_LEFT_20_PENALTY=0.320610
PRIMER_RIGHT_20_PENALTY=0.473695
PRIMER_LEFT_20_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_20_SEQUENCE=TTCGAAGGGAGTCATTTGGT
PRIMER_LEFT_20=910,20
PRIMER_RIGHT_20=1635,20
PRIMER_LEFT_20_TM=59.679
PRIMER_RIGHT_20_TM=59.526
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=726
PRIMER_PAIR_21_PENALTY=0.808520
PRIMER_LEFT_21_PENALTY=0.562082
PRIMER_RIGHT_21_PENALTY=0.246438
PRIMER_LEFT_21_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_21_SEQUENCE=CCCCTGTTTTGGTTGTGTTC
PRIMER_LEFT_21=909,20
PRIMER_RIGHT_21=1355,20
PRIMER_LEFT_21_TM=60.562
PRIMER_RIGHT_21_TM=60.246
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=447
PRIMER_PAIR_22_PENALTY=0.808520
PRIMER_LEFT_22_PENALTY=0.562082
PRIMER_RIGHT_22_PENALTY=0.246438
PRIMER_LEFT_22_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_22_SEQUENCE=TCCCCTGTTTTGGTTGTGTT
PRIMER_LEFT_22=909,20
PRIMER_RIGHT_22=1356,20
PRIMER_LEFT_22_TM=60.562
PRIMER_RIGHT_22_TM=60.246
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.3000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=448
PRIMER_PAIR_23_PENALTY=0.808520
PRIMER_LEFT_23_PENALTY=0.562082
PRIMER_RIGHT_23_PENALTY=0.246438
PRIMER_LEFT_23_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_23_SEQUENCE=TTCCCCTGTTTTGGTTGTGT
PRIMER_LEFT_23=909,20
PRIMER_RIGHT_23=1357,20
PRIMER_LEFT_23_TM=60.562
PRIMER_RIGHT_23_TM=60.246
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=449
PRIMER_PAIR_24_PENALTY=0.825677
PRIMER_LEFT_24_PENALTY=0.605823
PRIMER_RIGHT_24_PENALTY=0.219854
PRIMER_LEFT_24_SEQUENCE=CAAGTTTGACACGACCACCA
PRIMER_RIGHT_24_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_24=527,20
PRIMER_RIGHT_24=1484,20
PRIMER_LEFT_24_TM=60.606
PRIMER_RIGHT_24_TM=60.220
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=958
PRIMER_PAIR_25_PENALTY=0.872892
PRIMER_LEFT_25_PENALTY=0.320610
PRIMER_RIGHT_25_PENALTY=0.552282
PRIMER_LEFT_25_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_25_SEQUENCE=CTTCAAGGGAACGAGGCTAA
PRIMER_LEFT_25=910,20
PRIMER_RIGHT_25=1786,20
PRIMER_LEFT_25_TM=59.679
PRIMER_RIGHT_25_TM=59.448
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=7.5000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=877
PRIMER_PAIR_26_PENALTY=0.880017
PRIMER_LEFT_26_PENALTY=0.562082
PRIMER_RIGHT_26_PENALTY=0.317935
PRIMER_LEFT_26_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_26_SEQUENCE=GGTTGTGTTCAGATGCCAGA
PRIMER_LEFT_26=909,20
PRIMER_RIGHT_26=1345,20
PRIMER_LEFT_26_TM=60.562
PRIMER_RIGHT_26_TM=59.682
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=437
PRIMER_PAIR_27_PENALTY=0.981326
PRIMER_LEFT_27_PENALTY=0.761472
PRIMER_RIGHT_27_PENALTY=0.219854
PRIMER_LEFT_27_SEQUENCE=GGATTCACCAAACCAAGTCC
PRIMER_RIGHT_27_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_27=658,20
PRIMER_RIGHT_27=1484,20
PRIMER_LEFT_27_TM=59.239
PRIMER_RIGHT_27_TM=60.220
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=827
PRIMER_PAIR_28_PENALTY=0.983156
PRIMER_LEFT_28_PENALTY=0.260757
PRIMER_RIGHT_28_PENALTY=0.722400
PRIMER_LEFT_28_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_28_SEQUENCE=TGGTTGTGTTCAGATGCCAG
PRIMER_LEFT_28=544,20
PRIMER_RIGHT_28=1346,20
PRIMER_LEFT_28_TM=60.261
PRIMER_RIGHT_28_TM=60.722
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.1000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=803
PRIMER_PAIR_29_PENALTY=0.987300
PRIMER_LEFT_29_PENALTY=0.767446
PRIMER_RIGHT_29_PENALTY=0.219854
PRIMER_LEFT_29_SEQUENCE=TGCAAGTTTGACACGACCAC
PRIMER_RIGHT_29_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_29=525,20
PRIMER_RIGHT_29=1484,20
PRIMER_LEFT_29_TM=60.767
PRIMER_RIGHT_29_TM=60.220
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=960
PRIMER_PAIR_30_PENALTY=1.001143
PRIMER_LEFT_30_PENALTY=0.562082
PRIMER_RIGHT_30_PENALTY=0.439061
PRIMER_LEFT_30_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_30_SEQUENCE=TCATTTGGTTTTTGTGGCTG
PRIMER_LEFT_30=909,20
PRIMER_RIGHT_30=1624,20
PRIMER_LEFT_30_TM=60.562
PRIMER_RIGHT_30_TM=59.561
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=716
PRIMER_PAIR_31_PENALTY=1.007910
PRIMER_LEFT_31_PENALTY=0.761472
PRIMER_RIGHT_31_PENALTY=0.246438
PRIMER_LEFT_31_SEQUENCE=GGATTCACCAAACCAAGTCC
PRIMER_RIGHT_31_SEQUENCE=CCCCTGTTTTGGTTGTGTTC
PRIMER_LEFT_31=658,20
PRIMER_RIGHT_31=1355,20
PRIMER_LEFT_31_TM=59.239
PRIMER_RIGHT_31_TM=60.246
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=7.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=698
PRIMER_PAIR_32_PENALTY=1.015504
PRIMER_LEFT_32_PENALTY=0.562082
PRIMER_RIGHT_32_PENALTY=0.453422
PRIMER_LEFT_32_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_32_SEQUENCE=TTTGGTTGTGTTCAGATGCC
PRIMER_LEFT_32=909,20
PRIMER_RIGHT_32=1348,20
PRIMER_LEFT_32_TM=60.562
PRIMER_RIGHT_32_TM=59.547
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=440
PRIMER_PAIR_33_PENALTY=1.016304
PRIMER_LEFT_33_PENALTY=0.260757
PRIMER_RIGHT_33_PENALTY=0.755547
PRIMER_LEFT_33_SEQUENCE=CCATGTCCAGCTCCAAGAGT
PRIMER_RIGHT_33_SEQUENCE=TTTCCCCTGTTTTGGTTGTG
PRIMER_LEFT_33=544,20
PRIMER_RIGHT_33=1358,20
PRIMER_LEFT_33_TM=60.261
PRIMER_RIGHT_33_TM=60.756
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.1000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=815
PRIMER_PAIR_34_PENALTY=1.035777
PRIMER_LEFT_34_PENALTY=0.562082
PRIMER_RIGHT_34_PENALTY=0.473695
PRIMER_LEFT_34_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_34_SEQUENCE=TCGAAGGGAGTCATTTGGTT
PRIMER_LEFT_34=909,20
PRIMER_RIGHT_34=1634,20
PRIMER_LEFT_34_TM=60.562
PRIMER_RIGHT_34_TM=59.526
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.3000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=726
PRIMER_PAIR_35_PENALTY=1.035777
PRIMER_LEFT_35_PENALTY=0.562082
PRIMER_RIGHT_35_PENALTY=0.473695
PRIMER_LEFT_35_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_35_SEQUENCE=TTCGAAGGGAGTCATTTGGT
PRIMER_LEFT_35=909,20
PRIMER_RIGHT_35=1635,20
PRIMER_LEFT_35_TM=60.562
PRIMER_RIGHT_35_TM=59.526
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.3000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=727
PRIMER_PAIR_36_PENALTY=1.043009
PRIMER_LEFT_36_PENALTY=0.320610
PRIMER_RIGHT_36_PENALTY=0.722400
PRIMER_LEFT_36_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_36_SEQUENCE=TGGTTGTGTTCAGATGCCAG
PRIMER_LEFT_36=910,20
PRIMER_RIGHT_36=1346,20
PRIMER_LEFT_36_TM=59.679
PRIMER_RIGHT_36_TM=60.722
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=437
PRIMER_PAIR_37_PENALTY=1.076157
PRIMER_LEFT_37_PENALTY=0.320610
PRIMER_RIGHT_37_PENALTY=0.755547
PRIMER_LEFT_37_SEQUENCE=CCTTCTTTGCCATCCTTTTG
PRIMER_RIGHT_37_SEQUENCE=TTTCCCCTGTTTTGGTTGTG
PRIMER_LEFT_37=910,20
PRIMER_RIGHT_37=1358,20
PRIMER_LEFT_37_TM=59.679
PRIMER_RIGHT_37_TM=60.756
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=449
PRIMER_PAIR_38_PENALTY=1.079407
PRIMER_LEFT_38_PENALTY=0.761472
PRIMER_RIGHT_38_PENALTY=0.317935
PRIMER_LEFT_38_SEQUENCE=GGATTCACCAAACCAAGTCC
PRIMER_RIGHT_38_SEQUENCE=GGTTGTGTTCAGATGCCAGA
PRIMER_LEFT_38=658,20
PRIMER_RIGHT_38=1345,20
PRIMER_LEFT_38_TM=59.239
PRIMER_RIGHT_38_TM=59.682
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=688
PRIMER_PAIR_39_PENALTY=1.114364
PRIMER_LEFT_39_PENALTY=0.562082
PRIMER_RIGHT_39_PENALTY=0.552282
PRIMER_LEFT_39_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_39_SEQUENCE=CTTCAAGGGAACGAGGCTAA
PRIMER_LEFT_39=909,20
PRIMER_RIGHT_39=1786,20
PRIMER_LEFT_39_TM=60.562
PRIMER_RIGHT_39_TM=59.448
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=7.5000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=878
PRIMER_PAIR_40_PENALTY=1.160572
PRIMER_LEFT_40_PENALTY=0.940718
PRIMER_RIGHT_40_PENALTY=0.219854
PRIMER_LEFT_40_SEQUENCE=TCTCAGGATTCACCAAACCA
PRIMER_RIGHT_40_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_40=653,20
PRIMER_RIGHT_40=1484,20
PRIMER_LEFT_40_TM=59.059
PRIMER_RIGHT_40_TM=60.220
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=832
PRIMER_PAIR_41_PENALTY=1.200533
PRIMER_LEFT_41_PENALTY=0.761472
PRIMER_RIGHT_41_PENALTY=0.439061
PRIMER_LEFT_41_SEQUENCE=GGATTCACCAAACCAAGTCC
PRIMER_RIGHT_41_SEQUENCE=TCATTTGGTTTTTGTGGCTG
PRIMER_LEFT_41=658,20
PRIMER_RIGHT_41=1624,20
PRIMER_LEFT_41_TM=59.239
PRIMER_RIGHT_41_TM=59.561
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=967
PRIMER_PAIR_42_PENALTY=1.219552
PRIMER_LEFT_42_PENALTY=0.999698
PRIMER_RIGHT_42_PENALTY=0.219854
PRIMER_LEFT_42_SEQUENCE=ACACCTGGTTTGACTCCTCC
PRIMER_RIGHT_42_SEQUENCE=TGATTATGCAGGGCTGTTGA
PRIMER_LEFT_42=781,20
PRIMER_RIGHT_42=1484,20
PRIMER_LEFT_42_TM=59.000
PRIMER_RIGHT_42_TM=60.220
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=704
PRIMER_PAIR_43_PENALTY=1.246136
PRIMER_LEFT_43_PENALTY=0.999698
PRIMER_RIGHT_43_PENALTY=0.246438
PRIMER_LEFT_43_SEQUENCE=ACACCTGGTTTGACTCCTCC
PRIMER_RIGHT_43_SEQUENCE=CCCCTGTTTTGGTTGTGTTC
PRIMER_LEFT_43=781,20
PRIMER_RIGHT_43=1355,20
PRIMER_LEFT_43_TM=59.000
PRIMER_RIGHT_43_TM=60.246
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=575
PRIMER_PAIR_44_PENALTY=1.246136
PRIMER_LEFT_44_PENALTY=0.999698
PRIMER_RIGHT_44_PENALTY=0.246438
PRIMER_LEFT_44_SEQUENCE=ACACCTGGTTTGACTCCTCC
PRIMER_RIGHT_44_SEQUENCE=TCCCCTGTTTTGGTTGTGTT
PRIMER_LEFT_44=781,20
PRIMER_RIGHT_44=1356,20
PRIMER_LEFT_44_TM=59.000
PRIMER_RIGHT_44_TM=60.246
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=576
PRIMER_PAIR_45_PENALTY=1.246136
PRIMER_LEFT_45_PENALTY=0.999698
PRIMER_RIGHT_45_PENALTY=0.246438
PRIMER_LEFT_45_SEQUENCE=ACACCTGGTTTGACTCCTCC
PRIMER_RIGHT_45_SEQUENCE=TTCCCCTGTTTTGGTTGTGT
PRIMER_LEFT_45=781,20
PRIMER_RIGHT_45=1357,20
PRIMER_LEFT_45_TM=59.000
PRIMER_RIGHT_45_TM=60.246
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=577
PRIMER_PAIR_46_PENALTY=1.251092
PRIMER_LEFT_46_PENALTY=1.045388
PRIMER_RIGHT_46_PENALTY=0.205704
PRIMER_LEFT_46_SEQUENCE=TCGCATGCCAAACATAAACA
PRIMER_RIGHT_46_SEQUENCE=TCAAGGGAACGAGGCTAATG
PRIMER_LEFT_46=815,20
PRIMER_RIGHT_46=1784,20
PRIMER_LEFT_46_TM=61.045
PRIMER_RIGHT_46_TM=60.206
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=970
PRIMER_PAIR_47_PENALTY=1.269579
PRIMER_LEFT_47_PENALTY=1.045388
PRIMER_RIGHT_47_PENALTY=0.224191
PRIMER_LEFT_47_SEQUENCE=TCGCATGCCAAACATAAACA
PRIMER_RIGHT_47_SEQUENCE=TGTTCTTCAAGGGAACGAGG
PRIMER_LEFT_47=815,20
PRIMER_RIGHT_47=1790,20
PRIMER_LEFT_47_TM=61.045
PRIMER_RIGHT_47_TM=60.224
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=976
PRIMER_PAIR_48_PENALTY=1.284482
PRIMER_LEFT_48_PENALTY=0.562082
PRIMER_RIGHT_48_PENALTY=0.722400
PRIMER_LEFT_48_SEQUENCE=GCCTTCTTTGCCATCCTTTT
PRIMER_RIGHT_48_SEQUENCE=TGGTTGTGTTCAGATGCCAG
PRIMER_LEFT_48=909,20
PRIMER_RIGHT_48=1346,20
PRIMER_LEFT_48_TM=60.562
PRIMER_RIGHT_48_TM=60.722
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=438
PRIMER_PAIR_49_PENALTY=1.286086
PRIMER_LEFT_49_PENALTY=0.260155
PRIMER_RIGHT_49_PENALTY=1.025930
PRIMER_LEFT_49_SEQUENCE=TTTGCAAGTTTGACACGACC
PRIMER_RIGHT_49_SEQUENCE=AATGATTATGCAGGGCTGTTG
PRIMER_LEFT_49=523,20
PRIMER_RIGHT_49=1486,21
PRIMER_LEFT_49_TM=59.740
PRIMER_RIGHT_49_TM=59.974
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=964
=
SEQUENCE_ID=MAGIC.175_1_17028043_17028058_tsu-0_ws-0_2962_831066_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_16991696_16992216_mt-0_zu-0_266667_45581_TRUE
SEQUENCE_TARGET=950,620
SEQUENCE_TEMPLATE=TTTAAGAAACTCAATACACTTTTTTGGGTATTTTTCTTCAGTGTTCANATAATACCCAACCTTTTAGAATTCGNTTGTAGANGTTTATTTCTGTCATTTTCNTCACTTTTTTANCGTCAGAAAATNACTACACTATTCATTANCTTCAAAATATTGCTTCTNTAAATTNATACGTTTTGGTTGTTTNNAAAAACATGGNAAAATTTGGTGAATATTTTGTGTGANTTAGTTTGTTTGATTGATNTGGAAGANANAAAGAAGTTATCTANATAAAAATCATATACAGTACATAGGNTNAATAGAAACTTACCATTATTACNNCCACCGCTTTGNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACCGTTATCACCACCACCGCTTTTTCCTCCGCTTCCACNGTGCTGGTTGTTACTTATGGAATATAGCTCCAAACCATTGATCATGGTNGGCAANACCGAGTCANCAGTAGACTTTAAGACTATTTGGGACCCCGAAGTGGCCTCTACATCTCTCAAAACGAGTTGAGTCGCTTTCCCAAAAACCGGTACGATGGGATGTGAACCCACTTGCATGTCGTCCAAGAAAACGTTGAACGATCGTTTCTGGGTCCGACCTAGACTCTGAGGCTCCGAGAAGTAAAGAGCCAAATATACCAGTGTAGCAGTTNAGGGGAGAGGCAGGTCGGAGATGATTAAGCCGTCTCCTGACAANGCCTTNGACATAACAATCTCTGGCGGCTTGTTAGACGCCCCGGTGATGTCTATAGATGGGGCTGAGGTCGATAGATCNGTCAGTGCGGTGTTGTCTCCGGACGCTGACGGAGACCATAGCCTACCGAAAGGATCTAACGGATAGCTAAAATAAGTTTCCAANAAACCAAGTTAACCAACAATAGAAAANTTATAATTACTAAACAAAATTTTGATATATGTACTTGAAATTATATAAAGAGTNANATATATATATGAATCAGTAAATGTTATCTNTAGCAANGATCGAAATCAAATANCAAGAAAATCAATGTTAAGGATGAAAAACATATGCTATTTTATCAAAAAAGAAAACAATATGNTAATTGCTACATTGCTAACGAGAACCCCAAACNTTGCATGACTAANNTTTTTNTTTTTTGCATGACTAATTAACTTTGTGTATACCTTATAAGCTTTTTGGCTCCGTACGTAATTCTTTGATAGAGAATGAGACCTTCTTCAGGACCAAGATCTTTGTACATGCCGTCATNGAAGCTGTAAACTTCAATGGAAGATANAAAGGGTTATCGGACGGAGACGTACGNAGAAGGCAAACGCTGATATTCTCACTCGCCGGAGCAAAAATCACCTCAGAGAAATAAAATGTGTCCTCGTCGTCGAGTNACGACTCAGTAATATCAANAGAATCACGGTGTTTCCCGTCNTAAACTATGTCGAAAGNNNNNNNNNNNNNNNNNNNNTTGTCATCGTAGTTCTNNNNNNNNNNCTTCGTCCTCACGAGGGTTTTCCTGCNTTTTGTCACCGGAATGTTGGTGTAGCANTTTGTTTGACCGGTTGGAAANTACCGGAGAGTATTGATCGGCTTTGTGGTGACGTCCGTAATATTAATGGANTCGCCGGTTGTGACGAAGCCNTTATCTCCGACCCATGTTANGTTATTGGAGTCCACGTAGGATCCCGTTGTTCCACAGTCAATGCTTATANCTNACGANAAAAAAAANAANAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.412954
PRIMER_LEFT_0_PENALTY=0.022341
PRIMER_RIGHT_0_PENALTY=0.390614
PRIMER_LEFT_0_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_0_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_0=741,20
PRIMER_RIGHT_0=1609,20
PRIMER_LEFT_0_TM=59.978
PRIMER_RIGHT_0_TM=59.609
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.0000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=869
PRIMER_PAIR_1_PENALTY=0.625835
PRIMER_LEFT_1_PENALTY=0.235222
PRIMER_RIGHT_1_PENALTY=0.390614
PRIMER_LEFT_1_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_1_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_1=734,20
PRIMER_RIGHT_1=1609,20
PRIMER_LEFT_1_TM=60.235
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=876
PRIMER_PAIR_2_PENALTY=0.834187
PRIMER_LEFT_2_PENALTY=0.022341
PRIMER_RIGHT_2_PENALTY=0.811846
PRIMER_LEFT_2_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_2_SEQUENCE=ATTGACTGTGGAACAACGGG
PRIMER_LEFT_2=741,20
PRIMER_RIGHT_2=1703,20
PRIMER_LEFT_2_TM=59.978
PRIMER_RIGHT_2_TM=60.812
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=963
PRIMER_PAIR_3_PENALTY=0.914923
PRIMER_LEFT_3_PENALTY=0.524309
PRIMER_RIGHT_3_PENALTY=0.390614
PRIMER_LEFT_3_SEQUENCE=AATCTCTGGCGGCTTGTTAG
PRIMER_RIGHT_3_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_3=740,20
PRIMER_RIGHT_3=1609,20
PRIMER_LEFT_3_TM=59.476
PRIMER_RIGHT_3_TM=59.609
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=870
PRIMER_PAIR_4_PENALTY=1.047068
PRIMER_LEFT_4_PENALTY=0.235222
PRIMER_RIGHT_4_PENALTY=0.811846
PRIMER_LEFT_4_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_4_SEQUENCE=ATTGACTGTGGAACAACGGG
PRIMER_LEFT_4=734,20
PRIMER_RIGHT_4=1703,20
PRIMER_LEFT_4_TM=60.235
PRIMER_RIGHT_4_TM=60.812
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=970
PRIMER_PAIR_5_PENALTY=1.116550
PRIMER_LEFT_5_PENALTY=0.022341
PRIMER_RIGHT_5_PENALTY=1.094209
PRIMER_LEFT_5_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_5_SEQUENCE=ATTACGGACGTCACCACAAAG
PRIMER_LEFT_5=741,20
PRIMER_RIGHT_5=1610,21
PRIMER_LEFT_5_TM=59.978
PRIMER_RIGHT_5_TM=59.906
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.0000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=870
PRIMER_PAIR_6_PENALTY=1.122400
PRIMER_LEFT_6_PENALTY=0.022341
PRIMER_RIGHT_6_PENALTY=1.100059
PRIMER_LEFT_6_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_6_SEQUENCE=ATTACGGACGTCACCACAAA
PRIMER_LEFT_6=741,20
PRIMER_RIGHT_6=1610,20
PRIMER_LEFT_6_TM=59.978
PRIMER_RIGHT_6_TM=58.900
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.0000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=870
PRIMER_PAIR_7_PENALTY=1.236994
PRIMER_LEFT_7_PENALTY=0.846380
PRIMER_RIGHT_7_PENALTY=0.390614
PRIMER_LEFT_7_SEQUENCE=GTCGGAGATGATTAAGCCGT
PRIMER_RIGHT_7_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_7=695,20
PRIMER_RIGHT_7=1609,20
PRIMER_LEFT_7_TM=59.154
PRIMER_RIGHT_7_TM=59.609
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=11.1000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=915
PRIMER_PAIR_8_PENALTY=1.240701
PRIMER_LEFT_8_PENALTY=0.850088
PRIMER_RIGHT_8_PENALTY=0.390614
PRIMER_LEFT_8_SEQUENCE=GGAGACCATAGCCTACCGAA
PRIMER_RIGHT_8_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_8=834,20
PRIMER_RIGHT_8=1609,20
PRIMER_LEFT_8_TM=59.150
PRIMER_RIGHT_8_TM=59.609
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.2000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=776
PRIMER_PAIR_9_PENALTY=1.329431
PRIMER_LEFT_9_PENALTY=0.235222
PRIMER_RIGHT_9_PENALTY=1.094209
PRIMER_LEFT_9_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_9_SEQUENCE=ATTACGGACGTCACCACAAAG
PRIMER_LEFT_9=734,20
PRIMER_RIGHT_9=1610,21
PRIMER_LEFT_9_TM=60.235
PRIMER_RIGHT_9_TM=59.906
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=11.4000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=877
PRIMER_PAIR_10_PENALTY=1.335280
PRIMER_LEFT_10_PENALTY=0.235222
PRIMER_RIGHT_10_PENALTY=1.100059
PRIMER_LEFT_10_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_10_SEQUENCE=ATTACGGACGTCACCACAAA
PRIMER_LEFT_10=734,20
PRIMER_RIGHT_10=1610,20
PRIMER_LEFT_10_TM=60.235
PRIMER_RIGHT_10_TM=58.900
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.4000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=877
PRIMER_PAIR_11_PENALTY=1.336155
PRIMER_LEFT_11_PENALTY=0.524309
PRIMER_RIGHT_11_PENALTY=0.811846
PRIMER_LEFT_11_SEQUENCE=AATCTCTGGCGGCTTGTTAG
PRIMER_RIGHT_11_SEQUENCE=ATTGACTGTGGAACAACGGG
PRIMER_LEFT_11=740,20
PRIMER_RIGHT_11=1703,20
PRIMER_LEFT_11_TM=59.476
PRIMER_RIGHT_11_TM=60.812
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=11.1000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=964
PRIMER_PAIR_12_PENALTY=1.402271
PRIMER_LEFT_12_PENALTY=0.022341
PRIMER_RIGHT_12_PENALTY=1.379931
PRIMER_LEFT_12_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_12_SEQUENCE=CACCACAAAGCCGATCAATAC
PRIMER_LEFT_12=741,20
PRIMER_RIGHT_12=1599,21
PRIMER_LEFT_12_TM=59.978
PRIMER_RIGHT_12_TM=60.380
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.0000
PRIMER_RIGHT_12_END_STABILITY=5.6000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=859
PRIMER_PAIR_13_PENALTY=1.439308
PRIMER_LEFT_13_PENALTY=0.022341
PRIMER_RIGHT_13_PENALTY=1.416967
PRIMER_LEFT_13_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_13_SEQUENCE=CCACAAAGCCGATCAATACTC
PRIMER_LEFT_13=741,20
PRIMER_RIGHT_13=1597,21
PRIMER_LEFT_13_TM=59.978
PRIMER_RIGHT_13_TM=59.583
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.0000
PRIMER_RIGHT_13_END_STABILITY=5.4000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=857
PRIMER_PAIR_14_PENALTY=1.576525
PRIMER_LEFT_14_PENALTY=1.185912
PRIMER_RIGHT_14_PENALTY=0.390614
PRIMER_LEFT_14_SEQUENCE=ATAACAATCTCTGGCGGCTT
PRIMER_RIGHT_14_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_14=735,20
PRIMER_RIGHT_14=1609,20
PRIMER_LEFT_14_TM=58.814
PRIMER_RIGHT_14_TM=59.609
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=875
PRIMER_PAIR_15_PENALTY=1.615152
PRIMER_LEFT_15_PENALTY=0.235222
PRIMER_RIGHT_15_PENALTY=1.379931
PRIMER_LEFT_15_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_15_SEQUENCE=CACCACAAAGCCGATCAATAC
PRIMER_LEFT_15=734,20
PRIMER_RIGHT_15=1599,21
PRIMER_LEFT_15_TM=60.235
PRIMER_RIGHT_15_TM=60.380
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=5.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=866
PRIMER_PAIR_16_PENALTY=1.618518
PRIMER_LEFT_16_PENALTY=0.524309
PRIMER_RIGHT_16_PENALTY=1.094209
PRIMER_LEFT_16_SEQUENCE=AATCTCTGGCGGCTTGTTAG
PRIMER_RIGHT_16_SEQUENCE=ATTACGGACGTCACCACAAAG
PRIMER_LEFT_16=740,20
PRIMER_RIGHT_16=1610,21
PRIMER_LEFT_16_TM=59.476
PRIMER_RIGHT_16_TM=59.906
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=871
PRIMER_PAIR_17_PENALTY=1.624368
PRIMER_LEFT_17_PENALTY=0.524309
PRIMER_RIGHT_17_PENALTY=1.100059
PRIMER_LEFT_17_SEQUENCE=AATCTCTGGCGGCTTGTTAG
PRIMER_RIGHT_17_SEQUENCE=ATTACGGACGTCACCACAAA
PRIMER_LEFT_17=740,20
PRIMER_RIGHT_17=1610,20
PRIMER_LEFT_17_TM=59.476
PRIMER_RIGHT_17_TM=58.900
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=871
PRIMER_PAIR_18_PENALTY=1.652189
PRIMER_LEFT_18_PENALTY=0.235222
PRIMER_RIGHT_18_PENALTY=1.416967
PRIMER_LEFT_18_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_18_SEQUENCE=CCACAAAGCCGATCAATACTC
PRIMER_LEFT_18=734,20
PRIMER_RIGHT_18=1597,21
PRIMER_LEFT_18_TM=60.235
PRIMER_RIGHT_18_TM=59.583
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=11.4000
PRIMER_RIGHT_18_END_STABILITY=5.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=864
PRIMER_PAIR_19_PENALTY=1.661934
PRIMER_LEFT_19_PENALTY=0.850088
PRIMER_RIGHT_19_PENALTY=0.811846
PRIMER_LEFT_19_SEQUENCE=GGAGACCATAGCCTACCGAA
PRIMER_RIGHT_19_SEQUENCE=ATTGACTGTGGAACAACGGG
PRIMER_LEFT_19=834,20
PRIMER_RIGHT_19=1703,20
PRIMER_LEFT_19_TM=59.150
PRIMER_RIGHT_19_TM=60.812
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=10.2000
PRIMER_RIGHT_19_END_STABILITY=11.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=870
PRIMER_PAIR_20_PENALTY=1.682581
PRIMER_LEFT_20_PENALTY=1.291968
PRIMER_RIGHT_20_PENALTY=0.390614
PRIMER_LEFT_20_SEQUENCE=TAACAATCTCTGGCGGCTTG
PRIMER_RIGHT_20_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_20=736,20
PRIMER_RIGHT_20=1609,20
PRIMER_LEFT_20_TM=61.292
PRIMER_RIGHT_20_TM=59.609
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=874
PRIMER_PAIR_21_PENALTY=1.847694
PRIMER_LEFT_21_PENALTY=1.457080
PRIMER_RIGHT_21_PENALTY=0.390614
PRIMER_LEFT_21_SEQUENCE=GGAGACCATAGCCTACCGAAA
PRIMER_RIGHT_21_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_21=834,21
PRIMER_RIGHT_21=1609,20
PRIMER_LEFT_21_TM=60.457
PRIMER_RIGHT_21_TM=59.609
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.0000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=776
PRIMER_PAIR_22_PENALTY=1.904240
PRIMER_LEFT_22_PENALTY=0.524309
PRIMER_RIGHT_22_PENALTY=1.379931
PRIMER_LEFT_22_SEQUENCE=AATCTCTGGCGGCTTGTTAG
PRIMER_RIGHT_22_SEQUENCE=CACCACAAAGCCGATCAATAC
PRIMER_LEFT_22=740,20
PRIMER_RIGHT_22=1599,21
PRIMER_LEFT_22_TM=59.476
PRIMER_RIGHT_22_TM=60.380
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.7000
PRIMER_RIGHT_22_END_STABILITY=5.6000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=860
PRIMER_PAIR_23_PENALTY=1.932611
PRIMER_LEFT_23_PENALTY=1.541998
PRIMER_RIGHT_23_PENALTY=0.390614
PRIMER_LEFT_23_SEQUENCE=GAGGCAGGTCGGAGATGATT
PRIMER_RIGHT_23_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_23=688,20
PRIMER_RIGHT_23=1609,20
PRIMER_LEFT_23_TM=61.542
PRIMER_RIGHT_23_TM=59.609
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=922
PRIMER_PAIR_24_PENALTY=1.940589
PRIMER_LEFT_24_PENALTY=0.846380
PRIMER_RIGHT_24_PENALTY=1.094209
PRIMER_LEFT_24_SEQUENCE=GTCGGAGATGATTAAGCCGT
PRIMER_RIGHT_24_SEQUENCE=ATTACGGACGTCACCACAAAG
PRIMER_LEFT_24=695,20
PRIMER_RIGHT_24=1610,21
PRIMER_LEFT_24_TM=59.154
PRIMER_RIGHT_24_TM=59.906
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=11.1000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=916
PRIMER_PAIR_25_PENALTY=1.941276
PRIMER_LEFT_25_PENALTY=0.524309
PRIMER_RIGHT_25_PENALTY=1.416967
PRIMER_LEFT_25_SEQUENCE=AATCTCTGGCGGCTTGTTAG
PRIMER_RIGHT_25_SEQUENCE=CCACAAAGCCGATCAATACTC
PRIMER_LEFT_25=740,20
PRIMER_RIGHT_25=1597,21
PRIMER_LEFT_25_TM=59.476
PRIMER_RIGHT_25_TM=59.583
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=5.7000
PRIMER_RIGHT_25_END_STABILITY=5.4000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=858
PRIMER_PAIR_26_PENALTY=1.944297
PRIMER_LEFT_26_PENALTY=0.850088
PRIMER_RIGHT_26_PENALTY=1.094209
PRIMER_LEFT_26_SEQUENCE=GGAGACCATAGCCTACCGAA
PRIMER_RIGHT_26_SEQUENCE=ATTACGGACGTCACCACAAAG
PRIMER_LEFT_26=834,20
PRIMER_RIGHT_26=1610,21
PRIMER_LEFT_26_TM=59.150
PRIMER_RIGHT_26_TM=59.906
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.2000
PRIMER_RIGHT_26_END_STABILITY=7.3000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=777
PRIMER_PAIR_27_PENALTY=1.946439
PRIMER_LEFT_27_PENALTY=0.846380
PRIMER_RIGHT_27_PENALTY=1.100059
PRIMER_LEFT_27_SEQUENCE=GTCGGAGATGATTAAGCCGT
PRIMER_RIGHT_27_SEQUENCE=ATTACGGACGTCACCACAAA
PRIMER_LEFT_27=695,20
PRIMER_RIGHT_27=1610,20
PRIMER_LEFT_27_TM=59.154
PRIMER_RIGHT_27_TM=58.900
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=11.1000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=916
PRIMER_PAIR_28_PENALTY=1.950147
PRIMER_LEFT_28_PENALTY=0.850088
PRIMER_RIGHT_28_PENALTY=1.100059
PRIMER_LEFT_28_SEQUENCE=GGAGACCATAGCCTACCGAA
PRIMER_RIGHT_28_SEQUENCE=ATTACGGACGTCACCACAAA
PRIMER_LEFT_28=834,20
PRIMER_RIGHT_28=1610,20
PRIMER_LEFT_28_TM=59.150
PRIMER_RIGHT_28_TM=58.900
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=10.2000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=777
PRIMER_PAIR_29_PENALTY=1.997758
PRIMER_LEFT_29_PENALTY=1.185912
PRIMER_RIGHT_29_PENALTY=0.811846
PRIMER_LEFT_29_SEQUENCE=ATAACAATCTCTGGCGGCTT
PRIMER_RIGHT_29_SEQUENCE=ATTGACTGTGGAACAACGGG
PRIMER_LEFT_29=735,20
PRIMER_RIGHT_29=1703,20
PRIMER_LEFT_29_TM=58.814
PRIMER_RIGHT_29_TM=60.812
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=11.1000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=969
PRIMER_PAIR_30_PENALTY=2.064581
PRIMER_LEFT_30_PENALTY=1.673968
PRIMER_RIGHT_30_PENALTY=0.390614
PRIMER_LEFT_30_SEQUENCE=GATTAAGCCGTCTCCTGACAA
PRIMER_RIGHT_30_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_30=704,21
PRIMER_RIGHT_30=1609,20
PRIMER_LEFT_30_TM=59.326
PRIMER_RIGHT_30_TM=59.609
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.3000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=906
PRIMER_PAIR_31_PENALTY=2.069013
PRIMER_LEFT_31_PENALTY=0.022341
PRIMER_RIGHT_31_PENALTY=2.046672
PRIMER_LEFT_31_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_31_SEQUENCE=TATTACGGACGTCACCACAAA
PRIMER_LEFT_31=741,20
PRIMER_RIGHT_31=1611,21
PRIMER_LEFT_31_TM=59.978
PRIMER_RIGHT_31_TM=58.953
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.0000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=871
PRIMER_PAIR_32_PENALTY=2.075447
PRIMER_LEFT_32_PENALTY=0.022341
PRIMER_RIGHT_32_PENALTY=2.053107
PRIMER_LEFT_32_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_32_SEQUENCE=GTCACCACAAAGCCGATCAA
PRIMER_LEFT_32=741,20
PRIMER_RIGHT_32=1601,20
PRIMER_LEFT_32_TM=59.978
PRIMER_RIGHT_32_TM=62.053
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.0000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=861
PRIMER_PAIR_33_PENALTY=2.097656
PRIMER_LEFT_33_PENALTY=1.707042
PRIMER_RIGHT_33_PENALTY=0.390614
PRIMER_LEFT_33_SEQUENCE=GCTCCGAGAAGTAAAGAGCC
PRIMER_RIGHT_33_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_33=640,20
PRIMER_RIGHT_33=1609,20
PRIMER_LEFT_33_TM=58.293
PRIMER_RIGHT_33_TM=59.609
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=970
PRIMER_PAIR_34_PENALTY=2.102664
PRIMER_LEFT_34_PENALTY=0.022341
PRIMER_RIGHT_34_PENALTY=2.080323
PRIMER_LEFT_34_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_34_SEQUENCE=TATTACGGACGTCACCACAAAG
PRIMER_LEFT_34=741,20
PRIMER_RIGHT_34=1611,22
PRIMER_LEFT_34_TM=59.978
PRIMER_RIGHT_34_TM=59.920
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.0000
PRIMER_RIGHT_34_END_STABILITY=7.3000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=871
PRIMER_PAIR_35_PENALTY=2.103814
PRIMER_LEFT_35_PENALTY=1.291968
PRIMER_RIGHT_35_PENALTY=0.811846
PRIMER_LEFT_35_SEQUENCE=TAACAATCTCTGGCGGCTTG
PRIMER_RIGHT_35_SEQUENCE=ATTGACTGTGGAACAACGGG
PRIMER_LEFT_35=736,20
PRIMER_RIGHT_35=1703,20
PRIMER_LEFT_35_TM=61.292
PRIMER_RIGHT_35_TM=60.812
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=11.1000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=968
PRIMER_PAIR_36_PENALTY=2.160921
PRIMER_LEFT_36_PENALTY=1.770308
PRIMER_RIGHT_36_PENALTY=0.390614
PRIMER_LEFT_36_SEQUENCE=GTCAGTGCGGTGTTGTCTCC
PRIMER_RIGHT_36_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_36=804,20
PRIMER_RIGHT_36=1609,20
PRIMER_LEFT_36_TM=61.770
PRIMER_RIGHT_36_TM=59.609
PRIMER_LEFT_36_GC_PERCENT=60.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=806
PRIMER_PAIR_37_PENALTY=2.186023
PRIMER_LEFT_37_PENALTY=1.795410
PRIMER_RIGHT_37_PENALTY=0.390614
PRIMER_LEFT_37_SEQUENCE=TGAGGCTCCGAGAAGTAAAGA
PRIMER_RIGHT_37_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_37=636,21
PRIMER_RIGHT_37=1609,20
PRIMER_LEFT_37_TM=59.205
PRIMER_RIGHT_37_TM=59.609
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=974
PRIMER_PAIR_38_PENALTY=2.217551
PRIMER_LEFT_38_PENALTY=1.826937
PRIMER_RIGHT_38_PENALTY=0.390614
PRIMER_LEFT_38_SEQUENCE=GACATAACAATCTCTGGCGG
PRIMER_RIGHT_38_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_38=732,20
PRIMER_RIGHT_38=1609,20
PRIMER_LEFT_38_TM=58.173
PRIMER_RIGHT_38_TM=59.609
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=12.9000
PRIMER_RIGHT_38_END_STABILITY=7.3000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=878
PRIMER_PAIR_39_PENALTY=2.221712
PRIMER_LEFT_39_PENALTY=0.022341
PRIMER_RIGHT_39_PENALTY=2.199371
PRIMER_LEFT_39_SEQUENCE=ATCTCTGGCGGCTTGTTAGA
PRIMER_RIGHT_39_SEQUENCE=CAAAGCCGATCAATACTCTCC
PRIMER_LEFT_39=741,20
PRIMER_RIGHT_39=1594,21
PRIMER_LEFT_39_TM=59.978
PRIMER_RIGHT_39_TM=58.801
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.0000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=854
PRIMER_PAIR_40_PENALTY=2.226311
PRIMER_LEFT_40_PENALTY=0.846380
PRIMER_RIGHT_40_PENALTY=1.379931
PRIMER_LEFT_40_SEQUENCE=GTCGGAGATGATTAAGCCGT
PRIMER_RIGHT_40_SEQUENCE=CACCACAAAGCCGATCAATAC
PRIMER_LEFT_40=695,20
PRIMER_RIGHT_40=1599,21
PRIMER_LEFT_40_TM=59.154
PRIMER_RIGHT_40_TM=60.380
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.1000
PRIMER_RIGHT_40_END_STABILITY=5.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=905
PRIMER_PAIR_41_PENALTY=2.230018
PRIMER_LEFT_41_PENALTY=0.850088
PRIMER_RIGHT_41_PENALTY=1.379931
PRIMER_LEFT_41_SEQUENCE=GGAGACCATAGCCTACCGAA
PRIMER_RIGHT_41_SEQUENCE=CACCACAAAGCCGATCAATAC
PRIMER_LEFT_41=834,20
PRIMER_RIGHT_41=1599,21
PRIMER_LEFT_41_TM=59.150
PRIMER_RIGHT_41_TM=60.380
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=10.2000
PRIMER_RIGHT_41_END_STABILITY=5.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=766
PRIMER_PAIR_42_PENALTY=2.263347
PRIMER_LEFT_42_PENALTY=0.846380
PRIMER_RIGHT_42_PENALTY=1.416967
PRIMER_LEFT_42_SEQUENCE=GTCGGAGATGATTAAGCCGT
PRIMER_RIGHT_42_SEQUENCE=CCACAAAGCCGATCAATACTC
PRIMER_LEFT_42=695,20
PRIMER_RIGHT_42=1597,21
PRIMER_LEFT_42_TM=59.154
PRIMER_RIGHT_42_TM=59.583
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=11.1000
PRIMER_RIGHT_42_END_STABILITY=5.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=903
PRIMER_PAIR_43_PENALTY=2.267055
PRIMER_LEFT_43_PENALTY=0.850088
PRIMER_RIGHT_43_PENALTY=1.416967
PRIMER_LEFT_43_SEQUENCE=GGAGACCATAGCCTACCGAA
PRIMER_RIGHT_43_SEQUENCE=CCACAAAGCCGATCAATACTC
PRIMER_LEFT_43=834,20
PRIMER_RIGHT_43=1597,21
PRIMER_LEFT_43_TM=59.150
PRIMER_RIGHT_43_TM=59.583
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=10.2000
PRIMER_RIGHT_43_END_STABILITY=5.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=764
PRIMER_PAIR_44_PENALTY=2.268926
PRIMER_LEFT_44_PENALTY=1.457080
PRIMER_RIGHT_44_PENALTY=0.811846
PRIMER_LEFT_44_SEQUENCE=GGAGACCATAGCCTACCGAAA
PRIMER_RIGHT_44_SEQUENCE=ATTGACTGTGGAACAACGGG
PRIMER_LEFT_44=834,21
PRIMER_RIGHT_44=1703,20
PRIMER_LEFT_44_TM=60.457
PRIMER_RIGHT_44_TM=60.812
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.0000
PRIMER_RIGHT_44_END_STABILITY=11.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=870
PRIMER_PAIR_45_PENALTY=2.280121
PRIMER_LEFT_45_PENALTY=1.185912
PRIMER_RIGHT_45_PENALTY=1.094209
PRIMER_LEFT_45_SEQUENCE=ATAACAATCTCTGGCGGCTT
PRIMER_RIGHT_45_SEQUENCE=ATTACGGACGTCACCACAAAG
PRIMER_LEFT_45=735,20
PRIMER_RIGHT_45=1610,21
PRIMER_LEFT_45_TM=58.814
PRIMER_RIGHT_45_TM=59.906
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.7000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=876
PRIMER_PAIR_46_PENALTY=2.281894
PRIMER_LEFT_46_PENALTY=0.235222
PRIMER_RIGHT_46_PENALTY=2.046672
PRIMER_LEFT_46_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_46_SEQUENCE=TATTACGGACGTCACCACAAA
PRIMER_LEFT_46=734,20
PRIMER_RIGHT_46=1611,21
PRIMER_LEFT_46_TM=60.235
PRIMER_RIGHT_46_TM=58.953
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=11.4000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=878
PRIMER_PAIR_47_PENALTY=2.285970
PRIMER_LEFT_47_PENALTY=1.185912
PRIMER_RIGHT_47_PENALTY=1.100059
PRIMER_LEFT_47_SEQUENCE=ATAACAATCTCTGGCGGCTT
PRIMER_RIGHT_47_SEQUENCE=ATTACGGACGTCACCACAAA
PRIMER_LEFT_47=735,20
PRIMER_RIGHT_47=1610,20
PRIMER_LEFT_47_TM=58.814
PRIMER_RIGHT_47_TM=58.900
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=876
PRIMER_PAIR_48_PENALTY=2.288328
PRIMER_LEFT_48_PENALTY=0.235222
PRIMER_RIGHT_48_PENALTY=2.053107
PRIMER_LEFT_48_SEQUENCE=CATAACAATCTCTGGCGGCT
PRIMER_RIGHT_48_SEQUENCE=GTCACCACAAAGCCGATCAA
PRIMER_LEFT_48=734,20
PRIMER_RIGHT_48=1601,20
PRIMER_LEFT_48_TM=60.235
PRIMER_RIGHT_48_TM=62.053
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=11.4000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=868
PRIMER_PAIR_49_PENALTY=2.293088
PRIMER_LEFT_49_PENALTY=1.902474
PRIMER_RIGHT_49_PENALTY=0.390614
PRIMER_LEFT_49_SEQUENCE=TCCGAGAAGTAAAGAGCCAAA
PRIMER_RIGHT_49_SEQUENCE=TTACGGACGTCACCACAAAG
PRIMER_LEFT_49=642,21
PRIMER_RIGHT_49=1609,20
PRIMER_LEFT_49_TM=59.098
PRIMER_RIGHT_49_TM=59.609
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.8000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=968
=
SEQUENCE_ID=MAGIC.105_4_10026113_10026177_ct-1_col-0_7143_120505_TRUE
SEQUENCE_TARGET=950,164
SEQUENCE_TEMPLATE=ATTCTTTCTCTCTCGCTTCCAATATNTTCTTTCCGGGAGTTAACGTCAACCAAACCGACGAGATGGAAGACGAGGAAAACAACGTGAGGCACAANAAAGTGTCATTAAAACGACTTGCTCACCTTAACAAACCGGAAATTCCGGTTTTGGTACTCGGTTCAATTGCTGCAATGGTTCATGGAACGGTGTTTCCCATCTTCGGTTTANTGCTTTCCANCTCAATCAATATGTTCTATGAACCAGCAAAGATACTGAAGAAAGATTCACATTTTTGGGCGCTGATTTATATCGCTCTCGGTTTAACCAATTTCGTCATGATCCCGGTCCAAAACTATTTCTTTGGAATCGCTGGAGGGAAATTGATTAAACGCATCCGATCAATGTGTTTTGATAAAGTGGTTCATCAAGAAATCAGTTGGTTCGATGACACAGCCAATTCCAGGTACTATAACTTTATATATATAANTAANCGNNGTATTTTGTATGTTTTGATTCTAATATTTATTTGCGTTTTGTTANCNNCGGTGCGATTGGAGCGAGANTGNTCCACGGATGCTTCAACAGTGAGGAGTTTGGTAGGAGATGCATTGGCGTTAATAGTACAAAACATAGCTACTGTAACCACTGGGTTGATAATAGCGTTTACAGCGAATTGGATCCTAGCTCTCATAGTTCTTGCTCTCTCACCATTTATTGTCATCCAAGGATATGCTCAAACCAAGTTCTTGACTGGTTTCAGCGCTGATGCTAAGGTTTTAATATAGTGGTTACAACATTTTTTTGAATATATCCCGGTTTAGTCGCGATTTCGGGTTTACTGATCGATTTTTTTTCTGGTTTAGGCGATGTATGAAGAAGCGAGTCAAGTGGCGAATGATGCAGTGAGCAGCATAAGAACGGTTGCATCTTTCTGTGCAGAGGAGAAAGTGATGGATTTATACCAACAAAAATGCGATGGACCGAAGAAGAACGGTNTCCGGTTAGGTCTTTTGAGCGGTGCNGGTTTCGGTTTCTCATTCTTTTTCCTNTATTGCATCAATTGTGTATGTTTCGTCAGTGGTGCCNGGTTGATTCAAATTGGTAAAGCCACATTTGGTGAAGTCTTCAAGGTAAGATGAGAAACATTTCTCGGTTTAATTAAGATCTGGTTCGGTTTAGTGATCTTGAGAGTGTATTTTTTACAGGTTTTCTTTGCATTGACGATTATGGCGATTGGAGTTTCTCAAACGAGTGCTATGGCACCTGATTCAAACAAAGCTAAAGACTCTGCAGCTTCGATTTTCGATATTCTCGATAGTACACCGAAGATNGATTCTAGCTCTGATGAAGGTACAACGTTACAAAACGTTAATGGTGATATCGAATTCCGACATGTTAGTTTCCGATACCCAATGCGACCGGACGTTCAGATTTTCAGAGATTTGTGCTTGACTATCCCTTCTGGAAAGGTAAAAAACAGAGCTTTTTANTTACCGGTTNAATCTGGTTCACCGGTTAATATTGGTTATTGGTTGTTTTTTCAGACCGTTGCACTTGTTGGAGAGAGCGGGAGCGGGAAATCAACGGTGATAAGCATGATTGAGAGGTTTTATAATCCAGATTCAGGCAAGATTTTGATCGATCAGGTGGAGATTCAGANATTTAAATTGAGTTGGTTAAGGCAACAGATGGGTTTGGTTAGTCAAGAACCGATTTTGTTCAACGAGACGATCAGATCAAACATAGCTTATGGTAAAACCGGTGGAGCCACAGAGGAAGAGATTATAGCAGCAGCAAAAGCCGCAAACGCACATAACTTCATCAGCTCATTGCCTCAGGGTTACGNTACGNCGGTTGGAGAACGCGGTGTTCAGCTATCGGGCGGTCAGAAACAGAGGATTGCGATTGCGCGCGCGATTTTGAAAGATCCTAAGATTTTGTTGCTTGATGAAGCGACGAGTGCATTGGACGCAGAATCTGAGCGTGTTGTTCAGGATGCACTTGATCGAGTTATGGTTAATCGAACCACTGTAGTTGTGGCTCATCGGCTTACGACGATTAAGAACGCAGACGTGATCGCGGTTG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.222215
PRIMER_LEFT_0_PENALTY=0.103187
PRIMER_RIGHT_0_PENALTY=0.119028
PRIMER_LEFT_0_SEQUENCE=GTAGGAGATGCATTGGCGTT
PRIMER_RIGHT_0_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_0=575,20
PRIMER_RIGHT_0=1535,20
PRIMER_LEFT_0_TM=60.103
PRIMER_RIGHT_0_TM=59.881
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=961
PRIMER_PAIR_1_PENALTY=0.546561
PRIMER_LEFT_1_PENALTY=0.354881
PRIMER_RIGHT_1_PENALTY=0.191680
PRIMER_LEFT_1_SEQUENCE=ATTTCTTTGGAATCGCTGGA
PRIMER_RIGHT_1_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_1=333,20
PRIMER_RIGHT_1=1226,20
PRIMER_LEFT_1_TM=59.645
PRIMER_RIGHT_1_TM=60.192
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=894
PRIMER_PAIR_2_PENALTY=0.580922
PRIMER_LEFT_2_PENALTY=0.389242
PRIMER_RIGHT_2_PENALTY=0.191680
PRIMER_LEFT_2_SEQUENCE=ACCAATTTCGTCATGATCCC
PRIMER_RIGHT_2_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_2=302,20
PRIMER_RIGHT_2=1226,20
PRIMER_LEFT_2_TM=59.611
PRIMER_RIGHT_2_TM=60.192
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=925
PRIMER_PAIR_3_PENALTY=0.763967
PRIMER_LEFT_3_PENALTY=0.103187
PRIMER_RIGHT_3_PENALTY=0.660780
PRIMER_LEFT_3_SEQUENCE=GTAGGAGATGCATTGGCGTT
PRIMER_RIGHT_3_SEQUENCE=ACAAGTGCAACGGTCTGAAA
PRIMER_LEFT_3=575,20
PRIMER_RIGHT_3=1536,20
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=59.339
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=962
PRIMER_PAIR_4_PENALTY=0.790250
PRIMER_LEFT_4_PENALTY=0.354881
PRIMER_RIGHT_4_PENALTY=0.435368
PRIMER_LEFT_4_SEQUENCE=ATTTCTTTGGAATCGCTGGA
PRIMER_RIGHT_4_SEQUENCE=CTCCAATCGCCATAATCGTC
PRIMER_LEFT_4=333,20
PRIMER_RIGHT_4=1217,20
PRIMER_LEFT_4_TM=59.645
PRIMER_RIGHT_4_TM=60.435
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=885
PRIMER_PAIR_5_PENALTY=0.824610
PRIMER_LEFT_5_PENALTY=0.389242
PRIMER_RIGHT_5_PENALTY=0.435368
PRIMER_LEFT_5_SEQUENCE=ACCAATTTCGTCATGATCCC
PRIMER_RIGHT_5_SEQUENCE=CTCCAATCGCCATAATCGTC
PRIMER_LEFT_5=302,20
PRIMER_RIGHT_5=1217,20
PRIMER_LEFT_5_TM=59.611
PRIMER_RIGHT_5_TM=60.435
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.3000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=916
PRIMER_PAIR_6_PENALTY=0.829753
PRIMER_LEFT_6_PENALTY=0.354881
PRIMER_RIGHT_6_PENALTY=0.474872
PRIMER_LEFT_6_SEQUENCE=ATTTCTTTGGAATCGCTGGA
PRIMER_RIGHT_6_SEQUENCE=GCCATAATCGTCAATGCAAA
PRIMER_LEFT_6=333,20
PRIMER_RIGHT_6=1209,20
PRIMER_LEFT_6_TM=59.645
PRIMER_RIGHT_6_TM=59.525
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=877
PRIMER_PAIR_7_PENALTY=0.864114
PRIMER_LEFT_7_PENALTY=0.389242
PRIMER_RIGHT_7_PENALTY=0.474872
PRIMER_LEFT_7_SEQUENCE=ACCAATTTCGTCATGATCCC
PRIMER_RIGHT_7_SEQUENCE=GCCATAATCGTCAATGCAAA
PRIMER_LEFT_7=302,20
PRIMER_RIGHT_7=1209,20
PRIMER_LEFT_7_TM=59.611
PRIMER_RIGHT_7_TM=59.525
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.3000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=908
PRIMER_PAIR_8_PENALTY=0.873796
PRIMER_LEFT_8_PENALTY=0.754768
PRIMER_RIGHT_8_PENALTY=0.119028
PRIMER_LEFT_8_SEQUENCE=GAGTCAAGTGGCGAATGATG
PRIMER_RIGHT_8_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_8=859,20
PRIMER_RIGHT_8=1535,20
PRIMER_LEFT_8_TM=59.245
PRIMER_RIGHT_8_TM=59.881
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=677
PRIMER_PAIR_9_PENALTY=0.939784
PRIMER_LEFT_9_PENALTY=0.354881
PRIMER_RIGHT_9_PENALTY=0.584903
PRIMER_LEFT_9_SEQUENCE=ATTTCTTTGGAATCGCTGGA
PRIMER_RIGHT_9_SEQUENCE=ACTCCAATCGCCATAATCGT
PRIMER_LEFT_9=333,20
PRIMER_RIGHT_9=1218,20
PRIMER_LEFT_9_TM=59.645
PRIMER_RIGHT_9_TM=59.415
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=886
PRIMER_PAIR_10_PENALTY=0.957980
PRIMER_LEFT_10_PENALTY=0.838952
PRIMER_RIGHT_10_PENALTY=0.119028
PRIMER_LEFT_10_SEQUENCE=TGAAGAAGCGAGTCAAGTGG
PRIMER_RIGHT_10_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_10=850,20
PRIMER_RIGHT_10=1535,20
PRIMER_LEFT_10_TM=59.161
PRIMER_RIGHT_10_TM=59.881
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=686
PRIMER_PAIR_11_PENALTY=0.974145
PRIMER_LEFT_11_PENALTY=0.389242
PRIMER_RIGHT_11_PENALTY=0.584903
PRIMER_LEFT_11_SEQUENCE=ACCAATTTCGTCATGATCCC
PRIMER_RIGHT_11_SEQUENCE=ACTCCAATCGCCATAATCGT
PRIMER_LEFT_11=302,20
PRIMER_RIGHT_11=1218,20
PRIMER_LEFT_11_TM=59.611
PRIMER_RIGHT_11_TM=59.415
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.3000
PRIMER_RIGHT_11_END_STABILITY=8.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=917
PRIMER_PAIR_12_PENALTY=1.053854
PRIMER_LEFT_12_PENALTY=0.862173
PRIMER_RIGHT_12_PENALTY=0.191680
PRIMER_LEFT_12_SEQUENCE=AGCGCTGATGCTAAGGTTTT
PRIMER_RIGHT_12_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_12=737,20
PRIMER_RIGHT_12=1226,20
PRIMER_LEFT_12_TM=59.138
PRIMER_RIGHT_12_TM=60.192
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=11.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=490
PRIMER_PAIR_13_PENALTY=1.075441
PRIMER_LEFT_13_PENALTY=0.103187
PRIMER_RIGHT_13_PENALTY=0.972254
PRIMER_LEFT_13_SEQUENCE=GTAGGAGATGCATTGGCGTT
PRIMER_RIGHT_13_SEQUENCE=GCCATAGCACTCGTTTGAGA
PRIMER_LEFT_13=575,20
PRIMER_RIGHT_13=1239,20
PRIMER_LEFT_13_TM=60.103
PRIMER_RIGHT_13_TM=59.028
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=665
PRIMER_PAIR_14_PENALTY=1.101982
PRIMER_LEFT_14_PENALTY=0.910302
PRIMER_RIGHT_14_PENALTY=0.191680
PRIMER_LEFT_14_SEQUENCE=GATGACACAGCCAATTCCAG
PRIMER_RIGHT_14_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_14=422,20
PRIMER_RIGHT_14=1226,20
PRIMER_LEFT_14_TM=59.090
PRIMER_RIGHT_14_TM=60.192
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=11.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=805
PRIMER_PAIR_15_PENALTY=1.162307
PRIMER_LEFT_15_PENALTY=1.043279
PRIMER_RIGHT_15_PENALTY=0.119028
PRIMER_LEFT_15_SEQUENCE=TGCAGAGGAGAAAGTGATGG
PRIMER_RIGHT_15_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_15=913,20
PRIMER_RIGHT_15=1535,20
PRIMER_LEFT_15_TM=58.957
PRIMER_RIGHT_15_TM=59.881
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=7.3000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=623
PRIMER_PAIR_16_PENALTY=1.173371
PRIMER_LEFT_16_PENALTY=1.054343
PRIMER_RIGHT_16_PENALTY=0.119028
PRIMER_LEFT_16_SEQUENCE=CCAAGGATATGCTCAAACCAA
PRIMER_RIGHT_16_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_16=700,21
PRIMER_RIGHT_16=1535,20
PRIMER_LEFT_16_TM=59.946
PRIMER_RIGHT_16_TM=59.881
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=836
PRIMER_PAIR_17_PENALTY=1.237501
PRIMER_LEFT_17_PENALTY=1.118473
PRIMER_RIGHT_17_PENALTY=0.119028
PRIMER_LEFT_17_SEQUENCE=ACGGATGCTTCAACAGTGAG
PRIMER_RIGHT_17_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_17=548,20
PRIMER_RIGHT_17=1535,20
PRIMER_LEFT_17_TM=58.882
PRIMER_RIGHT_17_TM=59.881
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=988
PRIMER_PAIR_18_PENALTY=1.246023
PRIMER_LEFT_18_PENALTY=1.054343
PRIMER_RIGHT_18_PENALTY=0.191680
PRIMER_LEFT_18_SEQUENCE=CCAAGGATATGCTCAAACCAA
PRIMER_RIGHT_18_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_18=700,21
PRIMER_RIGHT_18=1226,20
PRIMER_LEFT_18_TM=59.946
PRIMER_RIGHT_18_TM=60.192
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=11.4000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=527
PRIMER_PAIR_19_PENALTY=1.258452
PRIMER_LEFT_19_PENALTY=0.754768
PRIMER_RIGHT_19_PENALTY=0.503684
PRIMER_LEFT_19_SEQUENCE=GAGTCAAGTGGCGAATGATG
PRIMER_RIGHT_19_SEQUENCE=TGAGGCAATGAGCTGATGAA
PRIMER_LEFT_19=859,20
PRIMER_RIGHT_19=1815,20
PRIMER_LEFT_19_TM=59.245
PRIMER_RIGHT_19_TM=60.504
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=957
PRIMER_PAIR_20_PENALTY=1.259983
PRIMER_LEFT_20_PENALTY=1.140954
PRIMER_RIGHT_20_PENALTY=0.119028
PRIMER_LEFT_20_SEQUENCE=GTGCAGAGGAGAAAGTGATGG
PRIMER_RIGHT_20_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_20=912,21
PRIMER_RIGHT_20=1535,20
PRIMER_LEFT_20_TM=59.859
PRIMER_RIGHT_20_TM=59.881
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=624
PRIMER_PAIR_21_PENALTY=1.259983
PRIMER_LEFT_21_PENALTY=1.140954
PRIMER_RIGHT_21_PENALTY=0.119028
PRIMER_LEFT_21_SEQUENCE=GGATGCTTCAACAGTGAGGAG
PRIMER_RIGHT_21_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_21=550,21
PRIMER_RIGHT_21=1535,20
PRIMER_LEFT_21_TM=59.859
PRIMER_RIGHT_21_TM=59.881
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=986
PRIMER_PAIR_22_PENALTY=1.264646
PRIMER_LEFT_22_PENALTY=1.043279
PRIMER_RIGHT_22_PENALTY=0.221368
PRIMER_LEFT_22_SEQUENCE=TGCAGAGGAGAAAGTGATGG
PRIMER_RIGHT_22_SEQUENCE=GCAATCGCAATCCTCTGTTT
PRIMER_LEFT_22=913,20
PRIMER_RIGHT_22=1887,20
PRIMER_LEFT_22_TM=58.957
PRIMER_RIGHT_22_TM=60.221
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=975
PRIMER_PAIR_23_PENALTY=1.281485
PRIMER_LEFT_23_PENALTY=0.103187
PRIMER_RIGHT_23_PENALTY=1.178298
PRIMER_LEFT_23_SEQUENCE=GTAGGAGATGCATTGGCGTT
PRIMER_RIGHT_23_SEQUENCE=TCCAGAAGGGATAGTCAAGCA
PRIMER_LEFT_23=575,20
PRIMER_RIGHT_23=1444,21
PRIMER_LEFT_23_TM=60.103
PRIMER_RIGHT_23_TM=59.822
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.9000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=870
PRIMER_PAIR_24_PENALTY=1.297542
PRIMER_LEFT_24_PENALTY=0.862173
PRIMER_RIGHT_24_PENALTY=0.435368
PRIMER_LEFT_24_SEQUENCE=AGCGCTGATGCTAAGGTTTT
PRIMER_RIGHT_24_SEQUENCE=CTCCAATCGCCATAATCGTC
PRIMER_LEFT_24=737,20
PRIMER_RIGHT_24=1217,20
PRIMER_LEFT_24_TM=59.138
PRIMER_RIGHT_24_TM=60.435
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=481
PRIMER_PAIR_25_PENALTY=1.310153
PRIMER_LEFT_25_PENALTY=1.118473
PRIMER_RIGHT_25_PENALTY=0.191680
PRIMER_LEFT_25_SEQUENCE=ACGGATGCTTCAACAGTGAG
PRIMER_RIGHT_25_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_25=548,20
PRIMER_RIGHT_25=1226,20
PRIMER_LEFT_25_TM=58.882
PRIMER_RIGHT_25_TM=60.192
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=11.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=679
PRIMER_PAIR_26_PENALTY=1.327135
PRIMER_LEFT_26_PENALTY=0.354881
PRIMER_RIGHT_26_PENALTY=0.972254
PRIMER_LEFT_26_SEQUENCE=ATTTCTTTGGAATCGCTGGA
PRIMER_RIGHT_26_SEQUENCE=GCCATAGCACTCGTTTGAGA
PRIMER_LEFT_26=333,20
PRIMER_RIGHT_26=1239,20
PRIMER_LEFT_26_TM=59.645
PRIMER_RIGHT_26_TM=59.028
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=907
PRIMER_PAIR_27_PENALTY=1.332634
PRIMER_LEFT_27_PENALTY=1.140954
PRIMER_RIGHT_27_PENALTY=0.191680
PRIMER_LEFT_27_SEQUENCE=GGATGCTTCAACAGTGAGGAG
PRIMER_RIGHT_27_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_27=550,21
PRIMER_RIGHT_27=1226,20
PRIMER_LEFT_27_TM=59.859
PRIMER_RIGHT_27_TM=60.192
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=11.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=677
PRIMER_PAIR_28_PENALTY=1.337045
PRIMER_LEFT_28_PENALTY=0.862173
PRIMER_RIGHT_28_PENALTY=0.474872
PRIMER_LEFT_28_SEQUENCE=AGCGCTGATGCTAAGGTTTT
PRIMER_RIGHT_28_SEQUENCE=GCCATAATCGTCAATGCAAA
PRIMER_LEFT_28=737,20
PRIMER_RIGHT_28=1209,20
PRIMER_LEFT_28_TM=59.138
PRIMER_RIGHT_28_TM=59.525
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=8.8000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=473
PRIMER_PAIR_29_PENALTY=1.342635
PRIMER_LEFT_29_PENALTY=0.838952
PRIMER_RIGHT_29_PENALTY=0.503684
PRIMER_LEFT_29_SEQUENCE=TGAAGAAGCGAGTCAAGTGG
PRIMER_RIGHT_29_SEQUENCE=TGAGGCAATGAGCTGATGAA
PRIMER_LEFT_29=850,20
PRIMER_RIGHT_29=1815,20
PRIMER_LEFT_29_TM=59.161
PRIMER_RIGHT_29_TM=60.504
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=966
PRIMER_PAIR_30_PENALTY=1.343944
PRIMER_LEFT_30_PENALTY=1.224915
PRIMER_RIGHT_30_PENALTY=0.119028
PRIMER_LEFT_30_SEQUENCE=AACGGTTGCATCTTTCTGTG
PRIMER_RIGHT_30_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_30=895,20
PRIMER_RIGHT_30=1535,20
PRIMER_LEFT_30_TM=58.775
PRIMER_RIGHT_30_TM=59.881
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.3000
PRIMER_PAIR_30_COMPL_ANY=8.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=641
PRIMER_PAIR_31_PENALTY=1.345670
PRIMER_LEFT_31_PENALTY=0.910302
PRIMER_RIGHT_31_PENALTY=0.435368
PRIMER_LEFT_31_SEQUENCE=GATGACACAGCCAATTCCAG
PRIMER_RIGHT_31_SEQUENCE=CTCCAATCGCCATAATCGTC
PRIMER_LEFT_31=422,20
PRIMER_RIGHT_31=1217,20
PRIMER_LEFT_31_TM=59.090
PRIMER_RIGHT_31_TM=60.435
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=796
PRIMER_PAIR_32_PENALTY=1.355933
PRIMER_LEFT_32_PENALTY=0.103187
PRIMER_RIGHT_32_PENALTY=1.252746
PRIMER_LEFT_32_SEQUENCE=GTAGGAGATGCATTGGCGTT
PRIMER_RIGHT_32_SEQUENCE=TCGTCAATGCAAAGAAAACC
PRIMER_LEFT_32=575,20
PRIMER_RIGHT_32=1202,20
PRIMER_LEFT_32_TM=60.103
PRIMER_RIGHT_32_TM=58.747
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.9000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=8.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=628
PRIMER_PAIR_33_PENALTY=1.361496
PRIMER_LEFT_33_PENALTY=0.389242
PRIMER_RIGHT_33_PENALTY=0.972254
PRIMER_LEFT_33_SEQUENCE=ACCAATTTCGTCATGATCCC
PRIMER_RIGHT_33_SEQUENCE=GCCATAGCACTCGTTTGAGA
PRIMER_LEFT_33=302,20
PRIMER_RIGHT_33=1239,20
PRIMER_LEFT_33_TM=59.611
PRIMER_RIGHT_33_TM=59.028
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.3000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=938
PRIMER_PAIR_34_PENALTY=1.362322
PRIMER_LEFT_34_PENALTY=1.140954
PRIMER_RIGHT_34_PENALTY=0.221368
PRIMER_LEFT_34_SEQUENCE=GTGCAGAGGAGAAAGTGATGG
PRIMER_RIGHT_34_SEQUENCE=GCAATCGCAATCCTCTGTTT
PRIMER_LEFT_34=912,21
PRIMER_RIGHT_34=1887,20
PRIMER_LEFT_34_TM=59.859
PRIMER_RIGHT_34_TM=60.221
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=7.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=976
PRIMER_PAIR_35_PENALTY=1.385174
PRIMER_LEFT_35_PENALTY=0.910302
PRIMER_RIGHT_35_PENALTY=0.474872
PRIMER_LEFT_35_SEQUENCE=GATGACACAGCCAATTCCAG
PRIMER_RIGHT_35_SEQUENCE=GCCATAATCGTCAATGCAAA
PRIMER_LEFT_35=422,20
PRIMER_RIGHT_35=1209,20
PRIMER_LEFT_35_TM=59.090
PRIMER_RIGHT_35_TM=59.525
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.8000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=788
PRIMER_PAIR_36_PENALTY=1.396753
PRIMER_LEFT_36_PENALTY=1.277724
PRIMER_RIGHT_36_PENALTY=0.119028
PRIMER_LEFT_36_SEQUENCE=GGCGATGTATGAAGAAGCGA
PRIMER_RIGHT_36_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_36=841,20
PRIMER_RIGHT_36=1535,20
PRIMER_LEFT_36_TM=61.278
PRIMER_RIGHT_36_TM=59.881
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.9000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=695
PRIMER_PAIR_37_PENALTY=1.402186
PRIMER_LEFT_37_PENALTY=0.754768
PRIMER_RIGHT_37_PENALTY=0.647418
PRIMER_LEFT_37_SEQUENCE=GAGTCAAGTGGCGAATGATG
PRIMER_RIGHT_37_SEQUENCE=CGTAACCCTGAGGCAATGAG
PRIMER_LEFT_37=859,20
PRIMER_RIGHT_37=1823,20
PRIMER_LEFT_37_TM=59.245
PRIMER_RIGHT_37_TM=60.647
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=965
PRIMER_PAIR_38_PENALTY=1.415548
PRIMER_LEFT_38_PENALTY=0.754768
PRIMER_RIGHT_38_PENALTY=0.660780
PRIMER_LEFT_38_SEQUENCE=GAGTCAAGTGGCGAATGATG
PRIMER_RIGHT_38_SEQUENCE=ACAAGTGCAACGGTCTGAAA
PRIMER_LEFT_38=859,20
PRIMER_RIGHT_38=1536,20
PRIMER_LEFT_38_TM=59.245
PRIMER_RIGHT_38_TM=59.339
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.3000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=678
PRIMER_PAIR_39_PENALTY=1.446283
PRIMER_LEFT_39_PENALTY=1.224915
PRIMER_RIGHT_39_PENALTY=0.221368
PRIMER_LEFT_39_SEQUENCE=AACGGTTGCATCTTTCTGTG
PRIMER_RIGHT_39_SEQUENCE=GCAATCGCAATCCTCTGTTT
PRIMER_LEFT_39=895,20
PRIMER_RIGHT_39=1887,20
PRIMER_LEFT_39_TM=58.775
PRIMER_RIGHT_39_TM=60.221
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=993
PRIMER_PAIR_40_PENALTY=1.447076
PRIMER_LEFT_40_PENALTY=0.862173
PRIMER_RIGHT_40_PENALTY=0.584903
PRIMER_LEFT_40_SEQUENCE=AGCGCTGATGCTAAGGTTTT
PRIMER_RIGHT_40_SEQUENCE=ACTCCAATCGCCATAATCGT
PRIMER_LEFT_40=737,20
PRIMER_RIGHT_40=1218,20
PRIMER_LEFT_40_TM=59.138
PRIMER_RIGHT_40_TM=59.415
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=8.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=482
PRIMER_PAIR_41_PENALTY=1.456143
PRIMER_LEFT_41_PENALTY=1.264463
PRIMER_RIGHT_41_PENALTY=0.191680
PRIMER_LEFT_41_SEQUENCE=TCAGTTGGTTCGATGACACAG
PRIMER_RIGHT_41_SEQUENCE=TTTGAGAAACTCCAATCGCC
PRIMER_LEFT_41=411,21
PRIMER_RIGHT_41=1226,20
PRIMER_LEFT_41_TM=59.736
PRIMER_RIGHT_41_TM=60.192
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=11.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=816
PRIMER_PAIR_42_PENALTY=1.476158
PRIMER_LEFT_42_PENALTY=1.357130
PRIMER_RIGHT_42_PENALTY=0.119028
PRIMER_LEFT_42_SEQUENCE=GTCGCGATTTCGGGTTTACT
PRIMER_RIGHT_42_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_42=799,20
PRIMER_RIGHT_42=1535,20
PRIMER_LEFT_42_TM=61.357
PRIMER_RIGHT_42_TM=59.881
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.7000
PRIMER_RIGHT_42_END_STABILITY=7.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=737
PRIMER_PAIR_43_PENALTY=1.479889
PRIMER_LEFT_43_PENALTY=0.103187
PRIMER_RIGHT_43_PENALTY=1.376703
PRIMER_LEFT_43_SEQUENCE=GTAGGAGATGCATTGGCGTT
PRIMER_RIGHT_43_SEQUENCE=CGGAAACTAACATGTCGGAA
PRIMER_LEFT_43=575,20
PRIMER_RIGHT_43=1383,20
PRIMER_LEFT_43_TM=60.103
PRIMER_RIGHT_43_TM=58.623
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=10.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=809
PRIMER_PAIR_44_PENALTY=1.486370
PRIMER_LEFT_44_PENALTY=0.838952
PRIMER_RIGHT_44_PENALTY=0.647418
PRIMER_LEFT_44_SEQUENCE=TGAAGAAGCGAGTCAAGTGG
PRIMER_RIGHT_44_SEQUENCE=CGTAACCCTGAGGCAATGAG
PRIMER_LEFT_44=850,20
PRIMER_RIGHT_44=1823,20
PRIMER_LEFT_44_TM=59.161
PRIMER_RIGHT_44_TM=60.647
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=974
PRIMER_PAIR_45_PENALTY=1.489711
PRIMER_LEFT_45_PENALTY=1.054343
PRIMER_RIGHT_45_PENALTY=0.435368
PRIMER_LEFT_45_SEQUENCE=CCAAGGATATGCTCAAACCAA
PRIMER_RIGHT_45_SEQUENCE=CTCCAATCGCCATAATCGTC
PRIMER_LEFT_45=700,21
PRIMER_RIGHT_45=1217,20
PRIMER_LEFT_45_TM=59.946
PRIMER_RIGHT_45_TM=60.435
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.1000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=518
PRIMER_PAIR_46_PENALTY=1.492323
PRIMER_LEFT_46_PENALTY=1.373294
PRIMER_RIGHT_46_PENALTY=0.119028
PRIMER_LEFT_46_SEQUENCE=GCGATGTATGAAGAAGCGAG
PRIMER_RIGHT_46_SEQUENCE=CAAGTGCAACGGTCTGAAAA
PRIMER_LEFT_46=842,20
PRIMER_RIGHT_46=1535,20
PRIMER_LEFT_46_TM=58.627
PRIMER_RIGHT_46_TM=59.881
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.9000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=694
PRIMER_PAIR_47_PENALTY=1.495205
PRIMER_LEFT_47_PENALTY=0.910302
PRIMER_RIGHT_47_PENALTY=0.584903
PRIMER_LEFT_47_SEQUENCE=GATGACACAGCCAATTCCAG
PRIMER_RIGHT_47_SEQUENCE=ACTCCAATCGCCATAATCGT
PRIMER_LEFT_47=422,20
PRIMER_RIGHT_47=1218,20
PRIMER_LEFT_47_TM=59.090
PRIMER_RIGHT_47_TM=59.415
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=797
PRIMER_PAIR_48_PENALTY=1.499732
PRIMER_LEFT_48_PENALTY=0.838952
PRIMER_RIGHT_48_PENALTY=0.660780
PRIMER_LEFT_48_SEQUENCE=TGAAGAAGCGAGTCAAGTGG
PRIMER_RIGHT_48_SEQUENCE=ACAAGTGCAACGGTCTGAAA
PRIMER_LEFT_48=850,20
PRIMER_RIGHT_48=1536,20
PRIMER_LEFT_48_TM=59.161
PRIMER_RIGHT_48_TM=59.339
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=687
PRIMER_PAIR_49_PENALTY=1.504210
PRIMER_LEFT_49_PENALTY=0.103187
PRIMER_RIGHT_49_PENALTY=1.401024
PRIMER_LEFT_49_SEQUENCE=GTAGGAGATGCATTGGCGTT
PRIMER_RIGHT_49_SEQUENCE=GCCATAGCACTCGTTTGAGAA
PRIMER_LEFT_49=575,20
PRIMER_RIGHT_49=1239,21
PRIMER_LEFT_49_TM=60.103
PRIMER_RIGHT_49_TM=60.401
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=665
=
SEQUENCE_ID=MAGIC.446_3_1131740_1132120_tsu-0_ler-0_151734_38873_TRUE
SEQUENCE_TARGET=950,480
SEQUENCE_TEMPLATE=TAAAAATGANATTAAACTGTATAGAAATTATTGCGTGTTTATGTTTCTATGTGTGTGCGTGTGTGATCACACCGTGCATGTATGGTTTCGTGTTTAATTTGTATATAGATGTATCTGTATCACATATATTACATAAGTATAATGAAATAGTGGCCTACGATTTTGGATTTTAAAGAAGATCTTATGTTCTAAGAAGGTAGGCATTGCATCAAGATTTATAGTTAAGAAAGAAAAAAAGTGAAAAGAAAGCATTTTANNAACATCACAAAGAAAAGCATCTTTTACATGAATTTGGCTGAAATGTAATCTACATTATTCTAAGTGAGTTGTATAACAAACTAATATGAGAACAAGTNAATCAAAATGGTGAACAAATTTAAAGAATGNATAAGAAACATGAGTAGAAATNNNAANANANATATATATANNTCTTTAATTAATTCTTCTAGATCATAATAAGATTCTATGTTCTTTTTATTTATTTCTTTCATATCGTTTATAAGTAAACAAAAGAGAGTATGTTATTTGATAATTTAGACAAGAAATGTATAATCAAGTTTAGATACTTTCATTCTTTNGTTTTTTGGGTTTAAAGGTTTTGTTGAACGGTTGTAAAATCACTATCGAACCCATCTGCTANTATAGAACGAGGAATATTCTCATGAAGTACTTTTATAAAAGCATTGAAGTTCATGCTTAAGCAAATTAAGCATANAAGTTAACCCATCAAAATCTACTACTTTATATGTCAGTAGTGTTCAATGTCGTATTCGTAATGCTTTACTATGAAAAATGGCTTANACTCACGGAAAAAGGAAGAAANATAAATAAAATCATAATTTAGGTTCTTTACGCATTACGTATTTTGTTATTGTCTAGTGTTTTNTAGAGCATTNGTTTTTCAACAGTTCTACCTTTTTGTAAATGTGAGGTAATTTGAAGATATTTTAACTCGAAATTNAAAAATTCAACAATCTATCGTGAAATTTTTTACATAATTNATAGCNAAATATCCCACATACATATTAAAANNNNNNNAAAAAAAAAAAAAAAAACAATCGTGAAACTGTTTTAGTAGCCCATTGGGNCCAATACNAATGANGCCTAATTTATNAAAAAATCTAAAAGTACACCCACTGGCCCATTAATATCTGAAATGTCTTANTTTTTTTCTTTTTTTGGGAACAAAGAAATGTCTTACATTAATTTAGTGATNAAATTTTGGTAGTTACCTTGTCTGANATGTCTTTGATTTTAGAGATTTATCCACNGGTAAAACTTCAAAAAATAGTTTTTTCTTTTCTTTTCTTTTTTCCAAAAACTTCAAGAATATAGATTAAAGGCTCAATACCTCAAATTATCAATAATAAGGTCGTTTCGNGTTTTCATAAACGAGGAATGTCACAATTTAAAATTGGCTTATGGTTTGACGCTATGATAAATTAATAAGGGTCCATCAATTGTNNNNAAAAAAAAAAAAAAAAATGNCAATTGTGAAAGAATATATATACTATATTTTTAATAGTGAAAGAATATTAAACTTCTCTATGTCTCTCTANTAAATTTTAAATACAATGTACATACAAGTTTCACCCACTTCACCAACTAGAACTCAATTCCTTTATTATTATTCACATTTCAATGTCTGCNGAATACCTCAACAAACTTTCTTCTGTACTCTTCCCATCCTTCACATGTTAATGATCCGTTGCCACAGTAATGTATAGNGACAAGGATTTTTNCNANAAGTACAGAGACACATATCATTACAATAACATGCAACAGTNTTAATTCTTCGTGTATATAATATAGTCCGGCAAAGAAATAAATAAGAGTTAAACTGTGACTCCTCATANTTTAAAATAGACTTNTTCTTCTTTTNTTTTTTTTCAAATTAAAGGNNNNAAAAAAAAATCGGTTGCGTTTTAACTGGAGAGTTAAGTCATCTGCTGCCACTCTTAAATTTTCNCACATATATTCATTTTTCGCACGTGATTTCGCCATATCATCATAATATCGATAATTATTTTAGTAATCAATTTTCTTAAGNGCTGCGAGGGTTTATTACTCTTCTGAACCTAAATACAGAATACAGTACATTTAGAGCAAATAATTAATGGCTCATGGTTGACNGAAAACAATNATTATTGGCTCATAAGTCATATATACNCTTTAAATGATTNCTGNATTAATTGAGANGAAACCNTTTGTGTATGTATGTTATGCTTTTTGTTAGTGAAGTACTATCGTTTATAGCCATGAGTTTTCAGTTACCATGCATGCATTTTGGTTGGTTTTGATTAAACTGAATCAATTAATGTGGATTAGGNTTATCTCAATTTTGGTTTCAATCGAGNAGATCCTCAATTTCCAGANANAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.260167
PRIMER_LEFT_0_PENALTY=1.291527
PRIMER_RIGHT_0_PENALTY=1.968639
PRIMER_LEFT_0_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_0_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_0=617,21
PRIMER_RIGHT_0=1603,21
PRIMER_LEFT_0_TM=59.708
PRIMER_RIGHT_0_TM=60.969
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=987
PRIMER_PAIR_1_PENALTY=3.261726
PRIMER_LEFT_1_PENALTY=1.291527
PRIMER_RIGHT_1_PENALTY=1.970198
PRIMER_LEFT_1_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_1_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_1=617,21
PRIMER_RIGHT_1=1602,20
PRIMER_LEFT_1_TM=59.708
PRIMER_RIGHT_1_TM=58.030
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=986
PRIMER_PAIR_2_PENALTY=3.261726
PRIMER_LEFT_2_PENALTY=1.291527
PRIMER_RIGHT_2_PENALTY=1.970198
PRIMER_LEFT_2_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_2_SEQUENCE=TTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_2=617,21
PRIMER_RIGHT_2=1604,20
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=58.030
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=988
PRIMER_PAIR_3_PENALTY=3.261726
PRIMER_LEFT_3_PENALTY=1.291527
PRIMER_RIGHT_3_PENALTY=1.970198
PRIMER_LEFT_3_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_3_SEQUENCE=AGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_3=617,21
PRIMER_RIGHT_3=1606,20
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=58.030
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=990
PRIMER_PAIR_4_PENALTY=3.337400
PRIMER_LEFT_4_PENALTY=1.291527
PRIMER_RIGHT_4_PENALTY=2.045872
PRIMER_LEFT_4_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_4_SEQUENCE=GTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_4=617,21
PRIMER_RIGHT_4=1605,21
PRIMER_LEFT_4_TM=59.708
PRIMER_RIGHT_4_TM=58.954
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=989
PRIMER_PAIR_5_PENALTY=3.337400
PRIMER_LEFT_5_PENALTY=1.291527
PRIMER_RIGHT_5_PENALTY=2.045872
PRIMER_LEFT_5_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_5_SEQUENCE=AGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_5=617,21
PRIMER_RIGHT_5=1606,21
PRIMER_LEFT_5_TM=59.708
PRIMER_RIGHT_5_TM=58.954
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=990
PRIMER_PAIR_6_PENALTY=3.353488
PRIMER_LEFT_6_PENALTY=1.291527
PRIMER_RIGHT_6_PENALTY=2.061961
PRIMER_LEFT_6_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_6_SEQUENCE=AGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_6=617,21
PRIMER_RIGHT_6=1606,22
PRIMER_LEFT_6_TM=59.708
PRIMER_RIGHT_6_TM=59.938
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=990
PRIMER_PAIR_7_PENALTY=3.421470
PRIMER_LEFT_7_PENALTY=1.291527
PRIMER_RIGHT_7_PENALTY=2.129943
PRIMER_LEFT_7_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_7_SEQUENCE=GTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_7=617,21
PRIMER_RIGHT_7=1605,20
PRIMER_LEFT_7_TM=59.708
PRIMER_RIGHT_7_TM=57.870
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=989
PRIMER_PAIR_8_PENALTY=3.658324
PRIMER_LEFT_8_PENALTY=1.291527
PRIMER_RIGHT_8_PENALTY=2.366796
PRIMER_LEFT_8_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_8_SEQUENCE=TCTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_8=617,21
PRIMER_RIGHT_8=1609,22
PRIMER_LEFT_8_TM=59.708
PRIMER_RIGHT_8_TM=59.633
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=993
PRIMER_PAIR_9_PENALTY=3.658324
PRIMER_LEFT_9_PENALTY=1.291527
PRIMER_RIGHT_9_PENALTY=2.366796
PRIMER_LEFT_9_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_9_SEQUENCE=TTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_9=617,21
PRIMER_RIGHT_9=1610,22
PRIMER_LEFT_9_TM=59.708
PRIMER_RIGHT_9_TM=59.633
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=994
PRIMER_PAIR_10_PENALTY=3.978934
PRIMER_LEFT_10_PENALTY=2.010294
PRIMER_RIGHT_10_PENALTY=1.968639
PRIMER_LEFT_10_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_10_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_10=616,22
PRIMER_RIGHT_10=1603,21
PRIMER_LEFT_10_TM=59.990
PRIMER_RIGHT_10_TM=60.969
PRIMER_LEFT_10_GC_PERCENT=45.455
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=988
PRIMER_PAIR_11_PENALTY=3.980493
PRIMER_LEFT_11_PENALTY=2.010294
PRIMER_RIGHT_11_PENALTY=1.970198
PRIMER_LEFT_11_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_11_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_11=616,22
PRIMER_RIGHT_11=1602,20
PRIMER_LEFT_11_TM=59.990
PRIMER_RIGHT_11_TM=58.030
PRIMER_LEFT_11_GC_PERCENT=45.455
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=987
PRIMER_PAIR_12_PENALTY=3.980493
PRIMER_LEFT_12_PENALTY=2.010294
PRIMER_RIGHT_12_PENALTY=1.970198
PRIMER_LEFT_12_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_12_SEQUENCE=TTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_12=616,22
PRIMER_RIGHT_12=1604,20
PRIMER_LEFT_12_TM=59.990
PRIMER_RIGHT_12_TM=58.030
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=989
PRIMER_PAIR_13_PENALTY=3.980493
PRIMER_LEFT_13_PENALTY=2.010294
PRIMER_RIGHT_13_PENALTY=1.970198
PRIMER_LEFT_13_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_13_SEQUENCE=AGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_13=616,22
PRIMER_RIGHT_13=1606,20
PRIMER_LEFT_13_TM=59.990
PRIMER_RIGHT_13_TM=58.030
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=991
PRIMER_PAIR_14_PENALTY=4.056167
PRIMER_LEFT_14_PENALTY=2.010294
PRIMER_RIGHT_14_PENALTY=2.045872
PRIMER_LEFT_14_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_14_SEQUENCE=GTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_14=616,22
PRIMER_RIGHT_14=1605,21
PRIMER_LEFT_14_TM=59.990
PRIMER_RIGHT_14_TM=58.954
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=990
PRIMER_PAIR_15_PENALTY=4.056167
PRIMER_LEFT_15_PENALTY=2.010294
PRIMER_RIGHT_15_PENALTY=2.045872
PRIMER_LEFT_15_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_15_SEQUENCE=AGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_15=616,22
PRIMER_RIGHT_15=1606,21
PRIMER_LEFT_15_TM=59.990
PRIMER_RIGHT_15_TM=58.954
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=991
PRIMER_PAIR_16_PENALTY=4.072255
PRIMER_LEFT_16_PENALTY=2.010294
PRIMER_RIGHT_16_PENALTY=2.061961
PRIMER_LEFT_16_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_16_SEQUENCE=AGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_16=616,22
PRIMER_RIGHT_16=1606,22
PRIMER_LEFT_16_TM=59.990
PRIMER_RIGHT_16_TM=59.938
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=991
PRIMER_PAIR_17_PENALTY=4.090515
PRIMER_LEFT_17_PENALTY=1.291527
PRIMER_RIGHT_17_PENALTY=2.798987
PRIMER_LEFT_17_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_17_SEQUENCE=TCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_17=617,21
PRIMER_RIGHT_17=1609,21
PRIMER_LEFT_17_TM=59.708
PRIMER_RIGHT_17_TM=58.201
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=993
PRIMER_PAIR_18_PENALTY=4.140237
PRIMER_LEFT_18_PENALTY=2.010294
PRIMER_RIGHT_18_PENALTY=2.129943
PRIMER_LEFT_18_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_18_SEQUENCE=GTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_18=616,22
PRIMER_RIGHT_18=1605,20
PRIMER_LEFT_18_TM=59.990
PRIMER_RIGHT_18_TM=57.870
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=990
PRIMER_PAIR_19_PENALTY=4.149574
PRIMER_LEFT_19_PENALTY=1.291527
PRIMER_RIGHT_19_PENALTY=2.858047
PRIMER_LEFT_19_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_19_SEQUENCE=CTAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_19=617,21
PRIMER_RIGHT_19=1608,22
PRIMER_LEFT_19_TM=59.708
PRIMER_RIGHT_19_TM=59.142
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=992
PRIMER_PAIR_20_PENALTY=4.172996
PRIMER_LEFT_20_PENALTY=1.291527
PRIMER_RIGHT_20_PENALTY=2.881468
PRIMER_LEFT_20_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_20_SEQUENCE=TAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_20=617,21
PRIMER_RIGHT_20=1607,21
PRIMER_LEFT_20_TM=59.708
PRIMER_RIGHT_20_TM=58.119
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=991
PRIMER_PAIR_21_PENALTY=4.202327
PRIMER_LEFT_21_PENALTY=2.233687
PRIMER_RIGHT_21_PENALTY=1.968639
PRIMER_LEFT_21_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_21_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_21=618,20
PRIMER_RIGHT_21=1603,21
PRIMER_LEFT_21_TM=57.766
PRIMER_RIGHT_21_TM=60.969
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=986
PRIMER_PAIR_22_PENALTY=4.203886
PRIMER_LEFT_22_PENALTY=2.233687
PRIMER_RIGHT_22_PENALTY=1.970198
PRIMER_LEFT_22_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_22_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_22=618,20
PRIMER_RIGHT_22=1602,20
PRIMER_LEFT_22_TM=57.766
PRIMER_RIGHT_22_TM=58.030
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=985
PRIMER_PAIR_23_PENALTY=4.203886
PRIMER_LEFT_23_PENALTY=2.233687
PRIMER_RIGHT_23_PENALTY=1.970198
PRIMER_LEFT_23_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_23_SEQUENCE=TTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_23=618,20
PRIMER_RIGHT_23=1604,20
PRIMER_LEFT_23_TM=57.766
PRIMER_RIGHT_23_TM=58.030
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=987
PRIMER_PAIR_24_PENALTY=4.203886
PRIMER_LEFT_24_PENALTY=2.233687
PRIMER_RIGHT_24_PENALTY=1.970198
PRIMER_LEFT_24_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_24_SEQUENCE=AGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_24=618,20
PRIMER_RIGHT_24=1606,20
PRIMER_LEFT_24_TM=57.766
PRIMER_RIGHT_24_TM=58.030
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=989
PRIMER_PAIR_25_PENALTY=4.279560
PRIMER_LEFT_25_PENALTY=2.233687
PRIMER_RIGHT_25_PENALTY=2.045872
PRIMER_LEFT_25_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_25_SEQUENCE=GTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_25=618,20
PRIMER_RIGHT_25=1605,21
PRIMER_LEFT_25_TM=57.766
PRIMER_RIGHT_25_TM=58.954
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=988
PRIMER_PAIR_26_PENALTY=4.279560
PRIMER_LEFT_26_PENALTY=2.233687
PRIMER_RIGHT_26_PENALTY=2.045872
PRIMER_LEFT_26_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_26_SEQUENCE=AGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_26=618,20
PRIMER_RIGHT_26=1606,21
PRIMER_LEFT_26_TM=57.766
PRIMER_RIGHT_26_TM=58.954
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=989
PRIMER_PAIR_27_PENALTY=4.287105
PRIMER_LEFT_27_PENALTY=1.291527
PRIMER_RIGHT_27_PENALTY=2.995578
PRIMER_LEFT_27_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_27_SEQUENCE=TAGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_27=617,21
PRIMER_RIGHT_27=1607,22
PRIMER_LEFT_27_TM=59.708
PRIMER_RIGHT_27_TM=59.004
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.455
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=991
PRIMER_PAIR_28_PENALTY=4.295648
PRIMER_LEFT_28_PENALTY=2.233687
PRIMER_RIGHT_28_PENALTY=2.061961
PRIMER_LEFT_28_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_28_SEQUENCE=AGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_28=618,20
PRIMER_RIGHT_28=1606,22
PRIMER_LEFT_28_TM=57.766
PRIMER_RIGHT_28_TM=59.938
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=989
PRIMER_PAIR_29_PENALTY=4.341027
PRIMER_LEFT_29_PENALTY=1.291527
PRIMER_RIGHT_29_PENALTY=3.049500
PRIMER_LEFT_29_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_29_SEQUENCE=TAGTTGGTGAAGTGGGTGAAACT
PRIMER_LEFT_29=617,21
PRIMER_RIGHT_29=1607,23
PRIMER_LEFT_29_TM=59.708
PRIMER_RIGHT_29_TM=59.950
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=43.478
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=991
PRIMER_PAIR_30_PENALTY=4.341027
PRIMER_LEFT_30_PENALTY=1.291527
PRIMER_RIGHT_30_PENALTY=3.049500
PRIMER_LEFT_30_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_30_SEQUENCE=CTAGTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_30=617,21
PRIMER_RIGHT_30=1608,23
PRIMER_LEFT_30_TM=59.708
PRIMER_RIGHT_30_TM=59.950
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=47.826
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=992
PRIMER_PAIR_31_PENALTY=4.363630
PRIMER_LEFT_31_PENALTY=2.233687
PRIMER_RIGHT_31_PENALTY=2.129943
PRIMER_LEFT_31_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_31_SEQUENCE=GTTGGTGAAGTGGGTGAAAC
PRIMER_LEFT_31=618,20
PRIMER_RIGHT_31=1605,20
PRIMER_LEFT_31_TM=57.766
PRIMER_RIGHT_31_TM=57.870
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=988
PRIMER_PAIR_32_PENALTY=4.377091
PRIMER_LEFT_32_PENALTY=2.010294
PRIMER_RIGHT_32_PENALTY=2.366796
PRIMER_LEFT_32_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_32_SEQUENCE=TCTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_32=616,22
PRIMER_RIGHT_32=1609,22
PRIMER_LEFT_32_TM=59.990
PRIMER_RIGHT_32_TM=59.633
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=994
PRIMER_PAIR_33_PENALTY=4.377091
PRIMER_LEFT_33_PENALTY=2.010294
PRIMER_RIGHT_33_PENALTY=2.366796
PRIMER_LEFT_33_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_33_SEQUENCE=TTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_33=616,22
PRIMER_RIGHT_33=1610,22
PRIMER_LEFT_33_TM=59.990
PRIMER_RIGHT_33_TM=59.633
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=995
PRIMER_PAIR_34_PENALTY=4.533694
PRIMER_LEFT_34_PENALTY=2.929208
PRIMER_RIGHT_34_PENALTY=1.604487
PRIMER_LEFT_34_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_34_SEQUENCE=AACATGTGAAGGATGGGAAGA
PRIMER_LEFT_34=802,20
PRIMER_RIGHT_34=1698,21
PRIMER_LEFT_34_TM=57.071
PRIMER_RIGHT_34_TM=59.396
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=897
PRIMER_PAIR_35_PENALTY=4.574138
PRIMER_LEFT_35_PENALTY=1.291527
PRIMER_RIGHT_35_PENALTY=3.282611
PRIMER_LEFT_35_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_35_SEQUENCE=CTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_35=617,21
PRIMER_RIGHT_35=1608,21
PRIMER_LEFT_35_TM=59.708
PRIMER_RIGHT_35_TM=57.717
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=4.574138
PRIMER_LEFT_36_PENALTY=1.291527
PRIMER_RIGHT_36_PENALTY=3.282611
PRIMER_LEFT_36_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_36_SEQUENCE=TTCTAGTTGGTGAAGTGGGTG
PRIMER_LEFT_36=617,21
PRIMER_RIGHT_36=1610,21
PRIMER_LEFT_36_TM=59.708
PRIMER_RIGHT_36_TM=57.717
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=994
PRIMER_PAIR_37_PENALTY=4.600484
PRIMER_LEFT_37_PENALTY=2.233687
PRIMER_RIGHT_37_PENALTY=2.366796
PRIMER_LEFT_37_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_37_SEQUENCE=TCTAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_37=618,20
PRIMER_RIGHT_37=1609,22
PRIMER_LEFT_37_TM=57.766
PRIMER_RIGHT_37_TM=59.633
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=992
PRIMER_PAIR_38_PENALTY=4.600484
PRIMER_LEFT_38_PENALTY=2.233687
PRIMER_RIGHT_38_PENALTY=2.366796
PRIMER_LEFT_38_SEQUENCE=CACTATCGAACCCATCTGCT
PRIMER_RIGHT_38_SEQUENCE=TTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_38=618,20
PRIMER_RIGHT_38=1610,22
PRIMER_LEFT_38_TM=57.766
PRIMER_RIGHT_38_TM=59.633
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=993
PRIMER_PAIR_39_PENALTY=4.666191
PRIMER_LEFT_39_PENALTY=1.291527
PRIMER_RIGHT_39_PENALTY=3.374664
PRIMER_LEFT_39_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_39_SEQUENCE=GTTCTAGTTGGTGAAGTGGGTG
PRIMER_LEFT_39=617,21
PRIMER_RIGHT_39=1611,22
PRIMER_LEFT_39_TM=59.708
PRIMER_RIGHT_39_TM=58.625
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=9.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=995
PRIMER_PAIR_40_PENALTY=4.696593
PRIMER_LEFT_40_PENALTY=1.291527
PRIMER_RIGHT_40_PENALTY=3.405066
PRIMER_LEFT_40_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_40_SEQUENCE=AGTTCTAGTTGGTGAAGTGGGTG
PRIMER_LEFT_40=617,21
PRIMER_RIGHT_40=1612,23
PRIMER_LEFT_40_TM=59.708
PRIMER_RIGHT_40_TM=59.595
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=47.826
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=9.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=996
PRIMER_PAIR_41_PENALTY=4.731350
PRIMER_LEFT_41_PENALTY=1.291527
PRIMER_RIGHT_41_PENALTY=3.439823
PRIMER_LEFT_41_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_41_SEQUENCE=TAGTTGGTGAAGTGGGTGAA
PRIMER_LEFT_41=617,21
PRIMER_RIGHT_41=1607,20
PRIMER_LEFT_41_TM=59.708
PRIMER_RIGHT_41_TM=56.560
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=991
PRIMER_PAIR_42_PENALTY=4.733258
PRIMER_LEFT_42_PENALTY=1.291527
PRIMER_RIGHT_42_PENALTY=3.441731
PRIMER_LEFT_42_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_42_SEQUENCE=GTTCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_42=617,21
PRIMER_RIGHT_42=1611,23
PRIMER_LEFT_42_TM=59.708
PRIMER_RIGHT_42_TM=60.442
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=47.826
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=995
PRIMER_PAIR_43_PENALTY=4.809282
PRIMER_LEFT_43_PENALTY=2.010294
PRIMER_RIGHT_43_PENALTY=2.798987
PRIMER_LEFT_43_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_43_SEQUENCE=TCTAGTTGGTGAAGTGGGTGA
PRIMER_LEFT_43=616,22
PRIMER_RIGHT_43=1609,21
PRIMER_LEFT_43_TM=59.990
PRIMER_RIGHT_43_TM=58.201
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=994
PRIMER_PAIR_44_PENALTY=4.856779
PRIMER_LEFT_44_PENALTY=1.291527
PRIMER_RIGHT_44_PENALTY=3.565251
PRIMER_LEFT_44_SEQUENCE=TCACTATCGAACCCATCTGCT
PRIMER_RIGHT_44_SEQUENCE=TGAGTTCTAGTTGGTGAAGTGGG
PRIMER_LEFT_44=617,21
PRIMER_RIGHT_44=1614,23
PRIMER_LEFT_44_TM=59.708
PRIMER_RIGHT_44_TM=60.565
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=47.826
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=998
PRIMER_PAIR_45_PENALTY=4.857871
PRIMER_LEFT_45_PENALTY=2.929208
PRIMER_RIGHT_45_PENALTY=1.928663
PRIMER_LEFT_45_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_45_SEQUENCE=CATGTGAAGGATGGGAAGAG
PRIMER_LEFT_45=802,20
PRIMER_RIGHT_45=1696,20
PRIMER_LEFT_45_TM=57.071
PRIMER_RIGHT_45_TM=58.071
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=895
PRIMER_PAIR_46_PENALTY=4.868341
PRIMER_LEFT_46_PENALTY=2.010294
PRIMER_RIGHT_46_PENALTY=2.858047
PRIMER_LEFT_46_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_46_SEQUENCE=CTAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_46=616,22
PRIMER_RIGHT_46=1608,22
PRIMER_LEFT_46_TM=59.990
PRIMER_RIGHT_46_TM=59.142
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=993
PRIMER_PAIR_47_PENALTY=4.891763
PRIMER_LEFT_47_PENALTY=2.010294
PRIMER_RIGHT_47_PENALTY=2.881468
PRIMER_LEFT_47_SEQUENCE=ATCACTATCGAACCCATCTGCT
PRIMER_RIGHT_47_SEQUENCE=TAGTTGGTGAAGTGGGTGAAA
PRIMER_LEFT_47=616,22
PRIMER_RIGHT_47=1607,21
PRIMER_LEFT_47_TM=59.990
PRIMER_RIGHT_47_TM=58.119
PRIMER_LEFT_47_GC_PERCENT=45.455
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=992
PRIMER_PAIR_48_PENALTY=4.897847
PRIMER_LEFT_48_PENALTY=2.929208
PRIMER_RIGHT_48_PENALTY=1.968639
PRIMER_LEFT_48_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_48_SEQUENCE=TGGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_48=802,20
PRIMER_RIGHT_48=1603,21
PRIMER_LEFT_48_TM=57.071
PRIMER_RIGHT_48_TM=60.969
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=802
PRIMER_PAIR_49_PENALTY=4.899406
PRIMER_LEFT_49_PENALTY=2.929208
PRIMER_RIGHT_49_PENALTY=1.970198
PRIMER_LEFT_49_SEQUENCE=CTCACGGAAAAAGGAAGAAA
PRIMER_RIGHT_49_SEQUENCE=GGTGAAGTGGGTGAAACTTG
PRIMER_LEFT_49=802,20
PRIMER_RIGHT_49=1602,20
PRIMER_LEFT_49_TM=57.071
PRIMER_RIGHT_49_TM=58.030
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=801
=
SEQUENCE_ID=MAGIC.287_4_4603961_4604061_oy-0_mt-0_12453_6961_TRUE
SEQUENCE_TARGET=950,200
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNCANATCTCTGTTCAAACATAAAGCTGTNTTAANACANGAACAANAATCNAATCTATTGAAATATNAGGAATCNTTAACANATTCANATTGNNGGTTACNNCTTTGATNAATACNAATTTGCCGNTTTAGAGATGATTNACGAANNNNNNNNNNNNNGATNCCCCNTANGAATCTGGNTTCTNCTANNACTTTGACGCAGATCTATATNTTAANAANNNAATCAAANTCTAGAANTNTANTNNACCAATTTNAAAGNACNAAAANCATAAATCCGANGNNNGAANNTAACTTACNTCTTTGTAGATCTNACAATTTGCTAACNACAAGTNTAGGATGANNANGAAATCATAAGNNNNNNNNNNNNTTTTGTGATTGAANCTTAGATCGACNGANNANNAANNNTTTTTGGNTTCGAATNATTNTTNTTTTTANAGACAACNTTNGTTNACNATNGAATANNNGTATATTAATATACAAAANTNNANAANCAATTCNNNATATAGTTNNGGCCNATNNTNACANNAAANTNGGCCNATATTACAATAAAANATNTTTNNNNNNATCTTATNNNNNNNNNNTTATATNTACNTTNTATNANCTTATGAGNTGTNAAATATGTCTTNGTGTATAAAATTTTAGATGGTTNATGTTGTTTTGTAAAAGTTTAGGTGTTACAAAANGAAAAATGACAATCATCATTGGTCAATANTGNTTAACAATCGGAAGACCATAATTCNATGTTTTTTNCCTTNNACTTTTGGGGTTTTCTACAANTTNCTATTAGTTATANNNNTAGTTTNAAAANTTTANAGCATNNAAGAATTTGANTNGAAAANGTTCAATATGTGTGGAAGTTAAACCATGTATATGTGTGAAAGTATCAATAATTGAGTGAAGATGGTGGCAACNCTGATCTTAAAACGCACAAATCTTATGAGNAAAANCAACAATGCTGNTATTAGAGGTTGNAGGAGATTTGTTTGGAACAAATTATTTGCTACTCTAAAATAAATTATTTTGAATGGTTATTCAGTANTTTTGACGAATGCAACATCAATTTCTTGTGTCCAAAAAGNNNNNNNNNNNNNNANCNTAANGAAGTGGTGGTGGCGGCGNGANTTGNGTGANCTTACATGNNNNNNNNNNNNAGAAAGCTCTCATGGGAAAAATGTCTAGACCAATAAGTTAAANTTGTTTACGTTAAACN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4642228_4642280_ler-0_bur-0_43789_7054_TRUE
SEQUENCE_TARGET=950,152
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNAAAGAATAAANAGAATAGNCNTTNACGAATANTATGANAGNNNAAAAAAACNTTTGCACNTNATTTATGACANTTTTACNCTAAANAAAAGNCAAACCNTCNTTNTTCCTNCTTCCATNTCTTTCTCTTTCTNTTTCTTNTTTCGACAAANTACATCTCTATTTTCTACCATTGTGCCTTATTCTTTGATNTCTNANANCATCATCACCTTCTCCATAACCACCTCCATTACAACAACCACCACAAGCTCTAGTAGATCATNTGACTATAGCCGTNAACNGTGTTGNTTTTCATCAATTTGGGATTCNGTGGTTCAACCATTGAGNTNTTCNGTTTATTTGGTCTATATANNATTTAGCTATTTCNGATATCGTTTCTTTTTANAATCTGAGATTTGCNTTCACCCTCAAGAGTTCAAGGTTTCNTTTCAATGTTATGTTATCATTTCATATATCANGTTANTTTNANNTTAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_1245093_1245370_col-0_ct-1_207721_145794_TRUE
SEQUENCE_TARGET=950,377
SEQUENCE_TEMPLATE=AGAGTATACACATTTGCATATCGAATTAGAGGAATACATTCTTTCGAAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATCAACAGAGACCTAATAACACCAAAACACATTTTACNTTTTTTNTTTTTGGTGGTNAAAACAGAGAAAAACCCATTTCGTAGTTGAGTCAAAGAACCCCAAATATCGAAAAAAGATAAACAAATCGAATTTTTGGGAAANAAAAAAAAAACAGAGAGAATTTTGGAGAGTGGTTAATGATTCTATAATGCTTTGAAGAAAGANAAAANAAAAAAANAAAANTCGAAAGGAAGATGATGATGAGAGATATTTCACGCGTGGGTGTTGAGAGAACATAAAAACAGAGTCATCACTCTAATTTTTGTTTTGTTCTCTAGGGAAAATCAAAAAGACCAAACAAAAATTTCAGTCAAATAATGGATCATGATTAAACCCACAAGAGAATTTCGATTACAAAACTCATTAGATAAAAAAAGTTTGAGACTTTATATAAACAAGTGTGTGATCGTATGTCTCTGACAAGACNAAGAGANAGAGGATGACTCACCTGAGATGGTAAGGAAAGAAGAAGATATTGTTGTTTTAGTAGAACTGAGAAAGGTTGTACTNGGAATGGAGTTTAGAACATGATANTGAAGAGACATGGCNTTAAAAAGCTCTGTTTTCTCTGTCTAAGTANTTANTGAAGAAGAGATGATGAAACTATTTGGNGANANAGAGAGAGAGAGAGAAGTCTTANNGGGTATTTATAAGGACAGAACCGACTTCACATCAGAGATTGTGTTTCATTTTACTCTTTGAGAAAGNAGAGAGAGAGAGNNNNGGATATGATGCATGGAATNTGTTTTTGTGGGATCTTCCAAAGCTACGGAATTGAAGGGTANATTTGGCTTTTTCTTCTATTTCNTATNCTCTTGTTTATATCTCTTTTACAACTTGNTTTTTTTTNATAAAAATAATGGNNTAAGNTNGATATCTTTGAAAGNNNNCACACACATACACATACGAGGGCTTCTAATCATATCATATCACAAACAATAATTTTTAAGGTCGTAAAAGATAATTAGGAAACAATATTTGTCGTGTGANAAGTCATATATGACTATTGCAATNGAATTATTATAAATTTGTCTTGGTGTAAAAAACAAAAAAAANNATGGTACTTATCTNGTGTCATATGGTACTAATCTATTTGGGATTCCNTTTTTTGTCAAGTTTAAGATTATTTTGTTTTAATCTTAAGTAACNAATNAGTTTCTGTTTACCGGAAACTCTAAATACATGGATTNATATATTTGTCGTGTGGACAGGTAACAATACATTACTAATATTGCAGTAAATNTTTATAAATGGTATTTGTCTCGGTCTAGATAAGAATTAGAAGTAGATGGTATATTACAATCTCTTGTCAAAACAGTATCAATCTGGTAGTAATTCCTAGTTTGTCAAGTTCAAAGAGGAGTTTTACGATTTCTTCNTTTTACTTNCATCTTATCTTAAGANTCTANANTCTCTCTATCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.277635
PRIMER_LEFT_0_PENALTY=1.246251
PRIMER_RIGHT_0_PENALTY=3.031385
PRIMER_LEFT_0_SEQUENCE=AAGGACAGAACCGACTTCACA
PRIMER_RIGHT_0_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_0=780,21
PRIMER_RIGHT_0=1346,20
PRIMER_LEFT_0_TM=59.754
PRIMER_RIGHT_0_TM=56.969
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.8000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=567
PRIMER_PAIR_1_PENALTY=4.461616
PRIMER_LEFT_1_PENALTY=1.430231
PRIMER_RIGHT_1_PENALTY=3.031385
PRIMER_LEFT_1_SEQUENCE=GGACAGAACCGACTTCACATC
PRIMER_RIGHT_1_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_1=782,21
PRIMER_RIGHT_1=1346,20
PRIMER_LEFT_1_TM=59.570
PRIMER_RIGHT_1_TM=56.969
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=565
PRIMER_PAIR_2_PENALTY=4.683807
PRIMER_LEFT_2_PENALTY=1.652423
PRIMER_RIGHT_2_PENALTY=3.031385
PRIMER_LEFT_2_SEQUENCE=TCCAAAGCTACGGAATTGAAG
PRIMER_RIGHT_2_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_2=888,21
PRIMER_RIGHT_2=1346,20
PRIMER_LEFT_2_TM=59.348
PRIMER_RIGHT_2_TM=56.969
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=459
PRIMER_PAIR_3_PENALTY=4.703650
PRIMER_LEFT_3_PENALTY=1.672265
PRIMER_RIGHT_3_PENALTY=3.031385
PRIMER_LEFT_3_SEQUENCE=AAAGCTACGGAATTGAAGGG
PRIMER_RIGHT_3_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_3=891,20
PRIMER_RIGHT_3=1346,20
PRIMER_LEFT_3_TM=58.328
PRIMER_RIGHT_3_TM=56.969
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=456
PRIMER_PAIR_4_PENALTY=4.756629
PRIMER_LEFT_4_PENALTY=1.725244
PRIMER_RIGHT_4_PENALTY=3.031385
PRIMER_LEFT_4_SEQUENCE=AGGACAGAACCGACTTCACA
PRIMER_RIGHT_4_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_4=781,20
PRIMER_RIGHT_4=1346,20
PRIMER_LEFT_4_TM=58.275
PRIMER_RIGHT_4_TM=56.969
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=566
PRIMER_PAIR_5_PENALTY=4.815698
PRIMER_LEFT_5_PENALTY=1.246251
PRIMER_RIGHT_5_PENALTY=3.569448
PRIMER_LEFT_5_SEQUENCE=AAGGACAGAACCGACTTCACA
PRIMER_RIGHT_5_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_5=780,21
PRIMER_RIGHT_5=1347,21
PRIMER_LEFT_5_TM=59.754
PRIMER_RIGHT_5_TM=57.431
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.8000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=568
PRIMER_PAIR_6_PENALTY=4.885614
PRIMER_LEFT_6_PENALTY=1.854229
PRIMER_RIGHT_6_PENALTY=3.031385
PRIMER_LEFT_6_SEQUENCE=AAAGCTACGGAATTGAAGGGT
PRIMER_RIGHT_6_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_6=891,21
PRIMER_RIGHT_6=1346,20
PRIMER_LEFT_6_TM=59.146
PRIMER_RIGHT_6_TM=56.969
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.1000
PRIMER_RIGHT_6_END_STABILITY=7.8000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=456
PRIMER_PAIR_7_PENALTY=4.983316
PRIMER_LEFT_7_PENALTY=1.951931
PRIMER_RIGHT_7_PENALTY=3.031385
PRIMER_LEFT_7_SEQUENCE=CAAAGCTACGGAATTGAAGGG
PRIMER_RIGHT_7_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_7=890,21
PRIMER_RIGHT_7=1346,20
PRIMER_LEFT_7_TM=60.952
PRIMER_RIGHT_7_TM=56.969
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=7.8000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=457
PRIMER_PAIR_8_PENALTY=4.983316
PRIMER_LEFT_8_PENALTY=1.951931
PRIMER_RIGHT_8_PENALTY=3.031385
PRIMER_LEFT_8_SEQUENCE=CCAAAGCTACGGAATTGAAGG
PRIMER_RIGHT_8_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_8=889,21
PRIMER_RIGHT_8=1346,20
PRIMER_LEFT_8_TM=60.952
PRIMER_RIGHT_8_TM=56.969
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.8000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=458
PRIMER_PAIR_9_PENALTY=4.999679
PRIMER_LEFT_9_PENALTY=1.430231
PRIMER_RIGHT_9_PENALTY=3.569448
PRIMER_LEFT_9_SEQUENCE=GGACAGAACCGACTTCACATC
PRIMER_RIGHT_9_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_9=782,21
PRIMER_RIGHT_9=1347,21
PRIMER_LEFT_9_TM=59.570
PRIMER_RIGHT_9_TM=57.431
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.3000
PRIMER_RIGHT_9_END_STABILITY=7.8000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=566
PRIMER_PAIR_10_PENALTY=5.124177
PRIMER_LEFT_10_PENALTY=2.092792
PRIMER_RIGHT_10_PENALTY=3.031385
PRIMER_LEFT_10_SEQUENCE=CAGAACCGACTTCACATCAGAG
PRIMER_RIGHT_10_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_10=785,22
PRIMER_RIGHT_10=1346,20
PRIMER_LEFT_10_TM=59.907
PRIMER_RIGHT_10_TM=56.969
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.8000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=562
PRIMER_PAIR_11_PENALTY=5.218859
PRIMER_LEFT_11_PENALTY=2.187474
PRIMER_RIGHT_11_PENALTY=3.031385
PRIMER_LEFT_11_SEQUENCE=AAGCTACGGAATTGAAGGGT
PRIMER_RIGHT_11_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_11=892,20
PRIMER_RIGHT_11=1346,20
PRIMER_LEFT_11_TM=57.813
PRIMER_RIGHT_11_TM=56.969
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.1000
PRIMER_RIGHT_11_END_STABILITY=7.8000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=455
PRIMER_PAIR_12_PENALTY=5.221870
PRIMER_LEFT_12_PENALTY=1.652423
PRIMER_RIGHT_12_PENALTY=3.569448
PRIMER_LEFT_12_SEQUENCE=TCCAAAGCTACGGAATTGAAG
PRIMER_RIGHT_12_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_12=888,21
PRIMER_RIGHT_12=1347,21
PRIMER_LEFT_12_TM=59.348
PRIMER_RIGHT_12_TM=57.431
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.8000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=460
PRIMER_PAIR_13_PENALTY=5.241713
PRIMER_LEFT_13_PENALTY=1.672265
PRIMER_RIGHT_13_PENALTY=3.569448
PRIMER_LEFT_13_SEQUENCE=AAAGCTACGGAATTGAAGGG
PRIMER_RIGHT_13_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_13=891,20
PRIMER_RIGHT_13=1347,21
PRIMER_LEFT_13_TM=58.328
PRIMER_RIGHT_13_TM=57.431
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=7.8000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=457
PRIMER_PAIR_14_PENALTY=5.257380
PRIMER_LEFT_14_PENALTY=2.225996
PRIMER_RIGHT_14_PENALTY=3.031385
PRIMER_LEFT_14_SEQUENCE=TAAGGACAGAACCGACTTCACA
PRIMER_RIGHT_14_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_14=779,22
PRIMER_RIGHT_14=1346,20
PRIMER_LEFT_14_TM=59.774
PRIMER_RIGHT_14_TM=56.969
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.8000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=568
PRIMER_PAIR_15_PENALTY=5.262657
PRIMER_LEFT_15_PENALTY=2.231272
PRIMER_RIGHT_15_PENALTY=3.031385
PRIMER_LEFT_15_SEQUENCE=GACAGAACCGACTTCACATCAG
PRIMER_RIGHT_15_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_15=783,22
PRIMER_RIGHT_15=1346,20
PRIMER_LEFT_15_TM=59.769
PRIMER_RIGHT_15_TM=56.969
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.8000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=564
PRIMER_PAIR_16_PENALTY=5.294692
PRIMER_LEFT_16_PENALTY=1.725244
PRIMER_RIGHT_16_PENALTY=3.569448
PRIMER_LEFT_16_SEQUENCE=AGGACAGAACCGACTTCACA
PRIMER_RIGHT_16_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_16=781,20
PRIMER_RIGHT_16=1347,21
PRIMER_LEFT_16_TM=58.275
PRIMER_RIGHT_16_TM=57.431
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.8000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=567
PRIMER_PAIR_17_PENALTY=5.319489
PRIMER_LEFT_17_PENALTY=2.288104
PRIMER_RIGHT_17_PENALTY=3.031385
PRIMER_LEFT_17_SEQUENCE=GACAGAACCGACTTCACATCA
PRIMER_RIGHT_17_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_17=783,21
PRIMER_RIGHT_17=1346,20
PRIMER_LEFT_17_TM=58.712
PRIMER_RIGHT_17_TM=56.969
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=7.8000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=564
PRIMER_PAIR_18_PENALTY=5.423676
PRIMER_LEFT_18_PENALTY=1.854229
PRIMER_RIGHT_18_PENALTY=3.569448
PRIMER_LEFT_18_SEQUENCE=AAAGCTACGGAATTGAAGGGT
PRIMER_RIGHT_18_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_18=891,21
PRIMER_RIGHT_18=1347,21
PRIMER_LEFT_18_TM=59.146
PRIMER_RIGHT_18_TM=57.431
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.1000
PRIMER_RIGHT_18_END_STABILITY=7.8000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=457
PRIMER_PAIR_19_PENALTY=5.513713
PRIMER_LEFT_19_PENALTY=2.482328
PRIMER_RIGHT_19_PENALTY=3.031385
PRIMER_LEFT_19_SEQUENCE=CAAAGCTACGGAATTGAAGG
PRIMER_RIGHT_19_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_19=890,20
PRIMER_RIGHT_19=1346,20
PRIMER_LEFT_19_TM=57.518
PRIMER_RIGHT_19_TM=56.969
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.8000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=457
PRIMER_PAIR_20_PENALTY=5.513713
PRIMER_LEFT_20_PENALTY=2.482328
PRIMER_RIGHT_20_PENALTY=3.031385
PRIMER_LEFT_20_SEQUENCE=CCAAAGCTACGGAATTGAAG
PRIMER_RIGHT_20_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_20=889,20
PRIMER_RIGHT_20=1346,20
PRIMER_LEFT_20_TM=57.518
PRIMER_RIGHT_20_TM=56.969
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.8000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=458
PRIMER_PAIR_21_PENALTY=5.521379
PRIMER_LEFT_21_PENALTY=1.951931
PRIMER_RIGHT_21_PENALTY=3.569448
PRIMER_LEFT_21_SEQUENCE=CAAAGCTACGGAATTGAAGGG
PRIMER_RIGHT_21_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_21=890,21
PRIMER_RIGHT_21=1347,21
PRIMER_LEFT_21_TM=60.952
PRIMER_RIGHT_21_TM=57.431
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=7.8000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=458
PRIMER_PAIR_22_PENALTY=5.521379
PRIMER_LEFT_22_PENALTY=1.951931
PRIMER_RIGHT_22_PENALTY=3.569448
PRIMER_LEFT_22_SEQUENCE=CCAAAGCTACGGAATTGAAGG
PRIMER_RIGHT_22_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_22=889,21
PRIMER_RIGHT_22=1347,21
PRIMER_LEFT_22_TM=60.952
PRIMER_RIGHT_22_TM=57.431
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.8000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=459
PRIMER_PAIR_23_PENALTY=5.584133
PRIMER_LEFT_23_PENALTY=2.552748
PRIMER_RIGHT_23_PENALTY=3.031385
PRIMER_LEFT_23_SEQUENCE=AGGACAGAACCGACTTCACATC
PRIMER_RIGHT_23_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_23=781,22
PRIMER_RIGHT_23=1346,20
PRIMER_LEFT_23_TM=60.553
PRIMER_RIGHT_23_TM=56.969
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.3000
PRIMER_RIGHT_23_END_STABILITY=7.8000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=566
PRIMER_PAIR_24_PENALTY=5.662239
PRIMER_LEFT_24_PENALTY=2.092792
PRIMER_RIGHT_24_PENALTY=3.569448
PRIMER_LEFT_24_SEQUENCE=CAGAACCGACTTCACATCAGAG
PRIMER_RIGHT_24_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_24=785,22
PRIMER_RIGHT_24=1347,21
PRIMER_LEFT_24_TM=59.907
PRIMER_RIGHT_24_TM=57.431
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=7.8000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=563
PRIMER_PAIR_25_PENALTY=5.705370
PRIMER_LEFT_25_PENALTY=1.246251
PRIMER_RIGHT_25_PENALTY=4.459120
PRIMER_LEFT_25_SEQUENCE=AAGGACAGAACCGACTTCACA
PRIMER_RIGHT_25_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_25=780,21
PRIMER_RIGHT_25=1348,22
PRIMER_LEFT_25_TM=59.754
PRIMER_RIGHT_25_TM=57.541
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.8000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=569
PRIMER_PAIR_26_PENALTY=5.756921
PRIMER_LEFT_26_PENALTY=2.187474
PRIMER_RIGHT_26_PENALTY=3.569448
PRIMER_LEFT_26_SEQUENCE=AAGCTACGGAATTGAAGGGT
PRIMER_RIGHT_26_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_26=892,20
PRIMER_RIGHT_26=1347,21
PRIMER_LEFT_26_TM=57.813
PRIMER_RIGHT_26_TM=57.431
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.1000
PRIMER_RIGHT_26_END_STABILITY=7.8000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=456
PRIMER_PAIR_27_PENALTY=5.795443
PRIMER_LEFT_27_PENALTY=2.225996
PRIMER_RIGHT_27_PENALTY=3.569448
PRIMER_LEFT_27_SEQUENCE=TAAGGACAGAACCGACTTCACA
PRIMER_RIGHT_27_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_27=779,22
PRIMER_RIGHT_27=1347,21
PRIMER_LEFT_27_TM=59.774
PRIMER_RIGHT_27_TM=57.431
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.8000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=569
PRIMER_PAIR_28_PENALTY=5.800720
PRIMER_LEFT_28_PENALTY=2.231272
PRIMER_RIGHT_28_PENALTY=3.569448
PRIMER_LEFT_28_SEQUENCE=GACAGAACCGACTTCACATCAG
PRIMER_RIGHT_28_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_28=783,22
PRIMER_RIGHT_28=1347,21
PRIMER_LEFT_28_TM=59.769
PRIMER_RIGHT_28_TM=57.431
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=7.8000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=565
PRIMER_PAIR_29_PENALTY=5.857552
PRIMER_LEFT_29_PENALTY=2.288104
PRIMER_RIGHT_29_PENALTY=3.569448
PRIMER_LEFT_29_SEQUENCE=GACAGAACCGACTTCACATCA
PRIMER_RIGHT_29_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_29=783,21
PRIMER_RIGHT_29=1347,21
PRIMER_LEFT_29_TM=58.712
PRIMER_RIGHT_29_TM=57.431
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.8000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=565
PRIMER_PAIR_30_PENALTY=5.889351
PRIMER_LEFT_30_PENALTY=1.430231
PRIMER_RIGHT_30_PENALTY=4.459120
PRIMER_LEFT_30_SEQUENCE=GGACAGAACCGACTTCACATC
PRIMER_RIGHT_30_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_30=782,21
PRIMER_RIGHT_30=1348,22
PRIMER_LEFT_30_TM=59.570
PRIMER_RIGHT_30_TM=57.541
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.3000
PRIMER_RIGHT_30_END_STABILITY=7.8000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=567
PRIMER_PAIR_31_PENALTY=6.051776
PRIMER_LEFT_31_PENALTY=2.482328
PRIMER_RIGHT_31_PENALTY=3.569448
PRIMER_LEFT_31_SEQUENCE=CAAAGCTACGGAATTGAAGG
PRIMER_RIGHT_31_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_31=890,20
PRIMER_RIGHT_31=1347,21
PRIMER_LEFT_31_TM=57.518
PRIMER_RIGHT_31_TM=57.431
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.8000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=458
PRIMER_PAIR_32_PENALTY=6.051776
PRIMER_LEFT_32_PENALTY=2.482328
PRIMER_RIGHT_32_PENALTY=3.569448
PRIMER_LEFT_32_SEQUENCE=CCAAAGCTACGGAATTGAAG
PRIMER_RIGHT_32_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_32=889,20
PRIMER_RIGHT_32=1347,21
PRIMER_LEFT_32_TM=57.518
PRIMER_RIGHT_32_TM=57.431
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=7.8000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=459
PRIMER_PAIR_33_PENALTY=6.078356
PRIMER_LEFT_33_PENALTY=3.046971
PRIMER_RIGHT_33_PENALTY=3.031385
PRIMER_LEFT_33_SEQUENCE=ATAAGGACAGAACCGACTTCACA
PRIMER_RIGHT_33_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_33=778,23
PRIMER_RIGHT_33=1346,20
PRIMER_LEFT_33_TM=60.047
PRIMER_RIGHT_33_TM=56.969
PRIMER_LEFT_33_GC_PERCENT=43.478
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.8000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=569
PRIMER_PAIR_34_PENALTY=6.088513
PRIMER_LEFT_34_PENALTY=3.057129
PRIMER_RIGHT_34_PENALTY=3.031385
PRIMER_LEFT_34_SEQUENCE=AGAACCGACTTCACATCAGAGA
PRIMER_RIGHT_34_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_34=786,22
PRIMER_RIGHT_34=1346,20
PRIMER_LEFT_34_TM=58.943
PRIMER_RIGHT_34_TM=56.969
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.8000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=561
PRIMER_PAIR_35_PENALTY=6.111542
PRIMER_LEFT_35_PENALTY=1.652423
PRIMER_RIGHT_35_PENALTY=4.459120
PRIMER_LEFT_35_SEQUENCE=TCCAAAGCTACGGAATTGAAG
PRIMER_RIGHT_35_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_35=888,21
PRIMER_RIGHT_35=1348,22
PRIMER_LEFT_35_TM=59.348
PRIMER_RIGHT_35_TM=57.541
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.8000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=461
PRIMER_PAIR_36_PENALTY=6.122196
PRIMER_LEFT_36_PENALTY=2.552748
PRIMER_RIGHT_36_PENALTY=3.569448
PRIMER_LEFT_36_SEQUENCE=AGGACAGAACCGACTTCACATC
PRIMER_RIGHT_36_SEQUENCE=ATTGTTACCTGTCCACACGAC
PRIMER_LEFT_36=781,22
PRIMER_RIGHT_36=1347,21
PRIMER_LEFT_36_TM=60.553
PRIMER_RIGHT_36_TM=57.431
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.3000
PRIMER_RIGHT_36_END_STABILITY=7.8000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=567
PRIMER_PAIR_37_PENALTY=6.131385
PRIMER_LEFT_37_PENALTY=1.672265
PRIMER_RIGHT_37_PENALTY=4.459120
PRIMER_LEFT_37_SEQUENCE=AAAGCTACGGAATTGAAGGG
PRIMER_RIGHT_37_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_37=891,20
PRIMER_RIGHT_37=1348,22
PRIMER_LEFT_37_TM=58.328
PRIMER_RIGHT_37_TM=57.541
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=7.8000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=458
PRIMER_PAIR_38_PENALTY=6.147800
PRIMER_LEFT_38_PENALTY=3.116415
PRIMER_RIGHT_38_PENALTY=3.031385
PRIMER_LEFT_38_SEQUENCE=TCACCTGAGATGGTAAGGAAAGA
PRIMER_RIGHT_38_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_38=573,23
PRIMER_RIGHT_38=1346,20
PRIMER_LEFT_38_TM=60.116
PRIMER_RIGHT_38_TM=56.969
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.8000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=774
PRIMER_PAIR_39_PENALTY=6.176885
PRIMER_LEFT_39_PENALTY=3.145500
PRIMER_RIGHT_39_PENALTY=3.031385
PRIMER_LEFT_39_SEQUENCE=GAACCGACTTCACATCAGAGA
PRIMER_RIGHT_39_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_39=787,21
PRIMER_RIGHT_39=1346,20
PRIMER_LEFT_39_TM=57.854
PRIMER_RIGHT_39_TM=56.969
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.8000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=560
PRIMER_PAIR_40_PENALTY=6.184364
PRIMER_LEFT_40_PENALTY=1.725244
PRIMER_RIGHT_40_PENALTY=4.459120
PRIMER_LEFT_40_SEQUENCE=AGGACAGAACCGACTTCACA
PRIMER_RIGHT_40_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_40=781,20
PRIMER_RIGHT_40=1348,22
PRIMER_LEFT_40_TM=58.275
PRIMER_RIGHT_40_TM=57.541
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.8000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=568
PRIMER_PAIR_41_PENALTY=6.245894
PRIMER_LEFT_41_PENALTY=3.214509
PRIMER_RIGHT_41_PENALTY=3.031385
PRIMER_LEFT_41_SEQUENCE=ACAGAACCGACTTCACATCAG
PRIMER_RIGHT_41_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_41=784,21
PRIMER_RIGHT_41=1346,20
PRIMER_LEFT_41_TM=57.785
PRIMER_RIGHT_41_TM=56.969
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.6000
PRIMER_RIGHT_41_END_STABILITY=7.8000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=563
PRIMER_PAIR_42_PENALTY=6.260287
PRIMER_LEFT_42_PENALTY=3.228902
PRIMER_RIGHT_42_PENALTY=3.031385
PRIMER_LEFT_42_SEQUENCE=AAGGACAGAACCGACTTCAC
PRIMER_RIGHT_42_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_42=780,20
PRIMER_RIGHT_42=1346,20
PRIMER_LEFT_42_TM=56.771
PRIMER_RIGHT_42_TM=56.969
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.8000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=567
PRIMER_PAIR_43_PENALTY=6.266312
PRIMER_LEFT_43_PENALTY=3.234927
PRIMER_RIGHT_43_PENALTY=3.031385
PRIMER_LEFT_43_SEQUENCE=CAGAACCGACTTCACATCAG
PRIMER_RIGHT_43_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_43=785,20
PRIMER_RIGHT_43=1346,20
PRIMER_LEFT_43_TM=56.765
PRIMER_RIGHT_43_TM=56.969
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=7.8000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=562
PRIMER_PAIR_44_PENALTY=6.313349
PRIMER_LEFT_44_PENALTY=1.854229
PRIMER_RIGHT_44_PENALTY=4.459120
PRIMER_LEFT_44_SEQUENCE=AAAGCTACGGAATTGAAGGGT
PRIMER_RIGHT_44_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_44=891,21
PRIMER_RIGHT_44=1348,22
PRIMER_LEFT_44_TM=59.146
PRIMER_RIGHT_44_TM=57.541
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=45.455
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.1000
PRIMER_RIGHT_44_END_STABILITY=7.8000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=458
PRIMER_PAIR_45_PENALTY=6.391845
PRIMER_LEFT_45_PENALTY=3.360461
PRIMER_RIGHT_45_PENALTY=3.031385
PRIMER_LEFT_45_SEQUENCE=CACCTGAGATGGTAAGGAAAGAA
PRIMER_RIGHT_45_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_45=574,23
PRIMER_RIGHT_45=1346,20
PRIMER_LEFT_45_TM=59.640
PRIMER_RIGHT_45_TM=56.969
PRIMER_LEFT_45_GC_PERCENT=43.478
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.8000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=773
PRIMER_PAIR_46_PENALTY=6.396443
PRIMER_LEFT_46_PENALTY=3.365058
PRIMER_RIGHT_46_PENALTY=3.031385
PRIMER_LEFT_46_SEQUENCE=GAACCGACTTCACATCAGAGATT
PRIMER_RIGHT_46_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_46=787,23
PRIMER_RIGHT_46=1346,20
PRIMER_LEFT_46_TM=59.635
PRIMER_RIGHT_46_TM=56.969
PRIMER_LEFT_46_GC_PERCENT=43.478
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.6000
PRIMER_RIGHT_46_END_STABILITY=7.8000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=560
PRIMER_PAIR_47_PENALTY=6.401804
PRIMER_LEFT_47_PENALTY=3.370420
PRIMER_RIGHT_47_PENALTY=3.031385
PRIMER_LEFT_47_SEQUENCE=GACTCACCTGAGATGGTAAGGAA
PRIMER_RIGHT_47_SEQUENCE=TTGTTACCTGTCCACACGAC
PRIMER_LEFT_47=570,23
PRIMER_RIGHT_47=1346,20
PRIMER_LEFT_47_TM=59.630
PRIMER_RIGHT_47_TM=56.969
PRIMER_LEFT_47_GC_PERCENT=47.826
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.8000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=777
PRIMER_PAIR_48_PENALTY=6.411051
PRIMER_LEFT_48_PENALTY=1.951931
PRIMER_RIGHT_48_PENALTY=4.459120
PRIMER_LEFT_48_SEQUENCE=CAAAGCTACGGAATTGAAGGG
PRIMER_RIGHT_48_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_48=890,21
PRIMER_RIGHT_48=1348,22
PRIMER_LEFT_48_TM=60.952
PRIMER_RIGHT_48_TM=57.541
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=7.8000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=459
PRIMER_PAIR_49_PENALTY=6.411051
PRIMER_LEFT_49_PENALTY=1.951931
PRIMER_RIGHT_49_PENALTY=4.459120
PRIMER_LEFT_49_SEQUENCE=CCAAAGCTACGGAATTGAAGG
PRIMER_RIGHT_49_SEQUENCE=TATTGTTACCTGTCCACACGAC
PRIMER_LEFT_49=889,21
PRIMER_RIGHT_49=1348,22
PRIMER_LEFT_49_TM=60.952
PRIMER_RIGHT_49_TM=57.541
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.8000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=460
=
SEQUENCE_ID=MAGIC.446_2_5933218_5933232_mt-0_ler-0_4009_272905_TRUE
SEQUENCE_TARGET=950,114
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTAGTTTTAAAGTGGCNTTTTTTTNGTGTGGTTCTAGNGTNTNTTNAGTNATTGGNTNTNCTATACANNANATCNTTANNNNNNNNNNNNNNNNNNNNNNCNATANAATGAAANATATACNNTANCNTTTNAAAGAATGAAAAAATAAATTAANAGACAGTTTCGNAGAAGANTTTTTTNNNNNNNNNNNAANTANATGANANATATGAAGAGATTAGTGNCGTGAAGGCNACTCATGGTTTGCNTCTTCTTCTTTAGTTTGTTGCTTTGTTTCAGCATTNGCCACTTTTTTGTCTATCAAAGGCTTTATCATTGCCTCCAAGTCATCTATATCTTGTGTGACTTTTTCCACCTGCNATTTNTNNGTTTTGACTTAGTCAATCACANTCANANNTNTATATATGTTTGNNNATTATTNGGTTCCAAAANATCCCCCAAATGATAAACCAATAAAAGNNACCCAAANANATANATCATATNTTCAAAATGTAATCNTTTACNTTAGCCTTTGTTAGTGTTNNNNNNNNNNNAGATTTTTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNATNAAGTGTNGTGGTNTACCTTGTGAAGGAAATCTTGGGTTANATGTGCTTCATGGGCAGCAACCTCTGAAATNTGTTCTAGAACCAAAGCCACTTGTTTTAGTTTANTTTTCTCAGGCAGTTTCTCTGCCATTTCTTCAGCCATTTCGTCAGTCGCNGTNGCTACTTTCTCCACCATTTCNNCTACAGCCTCTACCCCTTCCACAACCAACTCCGCCTCTCCTGCANNCANNNNATCATTCNANTTNNNNNNGTNCTNNNNNNANNNNANNNNNNNNNNNNNNNNNANNANNNNNNTANNANTNNNANACANNANNNNANCNTANANTNNNATNNAAGNNNNTTNTAGNNAANNNNNATGNNAGNTNTNANANNTNNATTACCTTCNATNCGTTTNAGTTTTTGNANCCTTTNGTTGTTCCAGAAAGATAACACTAANGATATCGCAGAACCTATAACCCATCTTCCCCTGCCNNTTANTCTCCANANNNNANNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNNNNNNNNNNNNNNNNNNACCANTGGTTTGGAGGAGANGGTTNCATCTTGGAGTCANTNGAAACATTACTNAAGTATCTCCCGGCATTANGNGGATTTGATAACTTGAGACCTTGTGGANANATTTGGTTATGATAGTCTCTTCCAAATCGAATAATCGNNGGACATGGATTCANANNTGTNTTCCTCTCNACTTTGTTNTATATTTGTAAGACNTCNGCAAGGCCCTGNCNATACACCGCAAAACCTCCGCTCNGCATTCTCCGACTTAGATACATNNTCAATCNGTTTTATNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNATTCAGTTTNTGTGTGANATTGGTTAAGCGTNTGNTTAAGATANAAGGCTTTTANGTGTAGTAANGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAAATNNNNNNNNNNNNNNNNAACAGAATTTACTTCAAAANGCNATTGAATTAAATAGGAAANCNTCNTTAAAAAATTNCATATTGNAANNTGGANNTATNNNNNNNNNNNATATTAGAATAAATAATTTTCAGCATNTTCNTCAAAGATTTTTTTAAAATAAAAATTAAATAAATTGACATTTTCTTCCTTTCTGGACATAAACCTAAATTAAGGAACATAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.343665
PRIMER_LEFT_0_PENALTY=0.012411
PRIMER_RIGHT_0_PENALTY=1.331254
PRIMER_LEFT_0_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_0_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_0=504,20
PRIMER_RIGHT_0=1114,21
PRIMER_LEFT_0_TM=59.988
PRIMER_RIGHT_0_TM=59.669
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=611
PRIMER_PAIR_1_PENALTY=1.663282
PRIMER_LEFT_1_PENALTY=0.332028
PRIMER_RIGHT_1_PENALTY=1.331254
PRIMER_LEFT_1_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_1_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_1=375,20
PRIMER_RIGHT_1=1114,21
PRIMER_LEFT_1_TM=59.668
PRIMER_RIGHT_1_TM=59.669
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=740
PRIMER_PAIR_2_PENALTY=2.094919
PRIMER_LEFT_2_PENALTY=0.012411
PRIMER_RIGHT_2_PENALTY=2.082508
PRIMER_LEFT_2_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_2_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_2=504,20
PRIMER_RIGHT_2=1112,22
PRIMER_LEFT_2_TM=59.988
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=609
PRIMER_PAIR_3_PENALTY=2.414536
PRIMER_LEFT_3_PENALTY=0.332028
PRIMER_RIGHT_3_PENALTY=2.082508
PRIMER_LEFT_3_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_3_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_3=375,20
PRIMER_RIGHT_3=1112,22
PRIMER_LEFT_3_TM=59.668
PRIMER_RIGHT_3_TM=59.917
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=738
PRIMER_PAIR_4_PENALTY=2.439670
PRIMER_LEFT_4_PENALTY=0.012411
PRIMER_RIGHT_4_PENALTY=2.427259
PRIMER_LEFT_4_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_4_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_4=504,20
PRIMER_RIGHT_4=1114,22
PRIMER_LEFT_4_TM=59.988
PRIMER_RIGHT_4_TM=60.427
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=611
PRIMER_PAIR_5_PENALTY=2.759287
PRIMER_LEFT_5_PENALTY=0.332028
PRIMER_RIGHT_5_PENALTY=2.427259
PRIMER_LEFT_5_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_5_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_5=375,20
PRIMER_RIGHT_5=1114,22
PRIMER_LEFT_5_TM=59.668
PRIMER_RIGHT_5_TM=60.427
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=740
PRIMER_PAIR_6_PENALTY=3.038153
PRIMER_LEFT_6_PENALTY=0.012411
PRIMER_RIGHT_6_PENALTY=3.025742
PRIMER_LEFT_6_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_6_SEQUENCE=AGGGGAAGATGGGTTATAGG
PRIMER_LEFT_6=504,20
PRIMER_RIGHT_6=1113,20
PRIMER_LEFT_6_TM=59.988
PRIMER_RIGHT_6_TM=56.974
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.1000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=610
PRIMER_PAIR_7_PENALTY=3.053471
PRIMER_LEFT_7_PENALTY=1.722217
PRIMER_RIGHT_7_PENALTY=1.331254
PRIMER_LEFT_7_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_7_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_7=374,20
PRIMER_RIGHT_7=1114,21
PRIMER_LEFT_7_TM=58.278
PRIMER_RIGHT_7_TM=59.669
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=741
PRIMER_PAIR_8_PENALTY=3.094702
PRIMER_LEFT_8_PENALTY=0.012411
PRIMER_RIGHT_8_PENALTY=3.082291
PRIMER_LEFT_8_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_8_SEQUENCE=GGGAAGATGGGTTATAGGTTCTG
PRIMER_LEFT_8=504,20
PRIMER_RIGHT_8=1111,23
PRIMER_LEFT_8_TM=59.988
PRIMER_RIGHT_8_TM=60.082
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=608
PRIMER_PAIR_9_PENALTY=3.119551
PRIMER_LEFT_9_PENALTY=0.332028
PRIMER_RIGHT_9_PENALTY=2.787523
PRIMER_LEFT_9_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_9_SEQUENCE=GGGGAAGATGGGTTATAGGTT
PRIMER_LEFT_9=375,20
PRIMER_RIGHT_9=1112,21
PRIMER_LEFT_9_TM=59.668
PRIMER_RIGHT_9_TM=58.212
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=738
PRIMER_PAIR_10_PENALTY=3.160417
PRIMER_LEFT_10_PENALTY=0.012411
PRIMER_RIGHT_10_PENALTY=3.148006
PRIMER_LEFT_10_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_10_SEQUENCE=AGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_10=504,20
PRIMER_RIGHT_10=1113,21
PRIMER_LEFT_10_TM=59.988
PRIMER_RIGHT_10_TM=57.852
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=610
PRIMER_PAIR_11_PENALTY=3.162311
PRIMER_LEFT_11_PENALTY=1.831057
PRIMER_RIGHT_11_PENALTY=1.331254
PRIMER_LEFT_11_SEQUENCE=CCTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_11_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_11=661,21
PRIMER_RIGHT_11=1114,21
PRIMER_LEFT_11_TM=60.831
PRIMER_RIGHT_11_TM=59.669
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.0000
PRIMER_RIGHT_11_END_STABILITY=7.1000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=454
PRIMER_PAIR_12_PENALTY=3.190666
PRIMER_LEFT_12_PENALTY=0.012411
PRIMER_RIGHT_12_PENALTY=3.178255
PRIMER_LEFT_12_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_12_SEQUENCE=GGGGAAGATGGGTTATAGGT
PRIMER_LEFT_12=504,20
PRIMER_RIGHT_12=1112,20
PRIMER_LEFT_12_TM=59.988
PRIMER_RIGHT_12_TM=56.822
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=609
PRIMER_PAIR_13_PENALTY=3.197244
PRIMER_LEFT_13_PENALTY=0.332028
PRIMER_RIGHT_13_PENALTY=2.865215
PRIMER_LEFT_13_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_13_SEQUENCE=AGGGGAAGATGGGTTATAGGTT
PRIMER_LEFT_13=375,20
PRIMER_RIGHT_13=1113,22
PRIMER_LEFT_13_TM=59.668
PRIMER_RIGHT_13_TM=59.135
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=739
PRIMER_PAIR_14_PENALTY=3.265944
PRIMER_LEFT_14_PENALTY=1.934690
PRIMER_RIGHT_14_PENALTY=1.331254
PRIMER_LEFT_14_SEQUENCE=GGCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_14_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_14=376,21
PRIMER_RIGHT_14=1114,21
PRIMER_LEFT_14_TM=60.935
PRIMER_RIGHT_14_TM=59.669
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=7.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=739
PRIMER_PAIR_15_PENALTY=3.265944
PRIMER_LEFT_15_PENALTY=1.934690
PRIMER_RIGHT_15_PENALTY=1.331254
PRIMER_LEFT_15_SEQUENCE=AAGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_15_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_15=374,21
PRIMER_RIGHT_15=1114,21
PRIMER_LEFT_15_TM=60.935
PRIMER_RIGHT_15_TM=59.669
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=741
PRIMER_PAIR_16_PENALTY=3.357770
PRIMER_LEFT_16_PENALTY=0.332028
PRIMER_RIGHT_16_PENALTY=3.025742
PRIMER_LEFT_16_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_16_SEQUENCE=AGGGGAAGATGGGTTATAGG
PRIMER_LEFT_16=375,20
PRIMER_RIGHT_16=1113,20
PRIMER_LEFT_16_TM=59.668
PRIMER_RIGHT_16_TM=56.974
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.1000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=739
PRIMER_PAIR_17_PENALTY=3.414319
PRIMER_LEFT_17_PENALTY=0.332028
PRIMER_RIGHT_17_PENALTY=3.082291
PRIMER_LEFT_17_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_17_SEQUENCE=GGGAAGATGGGTTATAGGTTCTG
PRIMER_LEFT_17=375,20
PRIMER_RIGHT_17=1111,23
PRIMER_LEFT_17_TM=59.668
PRIMER_RIGHT_17_TM=60.082
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=737
PRIMER_PAIR_18_PENALTY=3.433531
PRIMER_LEFT_18_PENALTY=2.102276
PRIMER_RIGHT_18_PENALTY=1.331254
PRIMER_LEFT_18_SEQUENCE=GCTTTATCATTGCCTCCAAGTC
PRIMER_RIGHT_18_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_18=377,22
PRIMER_RIGHT_18=1114,21
PRIMER_LEFT_18_TM=60.102
PRIMER_RIGHT_18_TM=59.669
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=738
PRIMER_PAIR_19_PENALTY=3.480034
PRIMER_LEFT_19_PENALTY=0.332028
PRIMER_RIGHT_19_PENALTY=3.148006
PRIMER_LEFT_19_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_19_SEQUENCE=AGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_19=375,20
PRIMER_RIGHT_19=1113,21
PRIMER_LEFT_19_TM=59.668
PRIMER_RIGHT_19_TM=57.852
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=739
PRIMER_PAIR_20_PENALTY=3.510283
PRIMER_LEFT_20_PENALTY=0.332028
PRIMER_RIGHT_20_PENALTY=3.178255
PRIMER_LEFT_20_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_20_SEQUENCE=GGGGAAGATGGGTTATAGGT
PRIMER_LEFT_20=375,20
PRIMER_RIGHT_20=1112,20
PRIMER_LEFT_20_TM=59.668
PRIMER_RIGHT_20_TM=56.822
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=738
PRIMER_PAIR_21_PENALTY=3.758362
PRIMER_LEFT_21_PENALTY=0.012411
PRIMER_RIGHT_21_PENALTY=3.745951
PRIMER_LEFT_21_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_21_SEQUENCE=GGGGAAGATGGGTTATAGGTTCT
PRIMER_LEFT_21=504,20
PRIMER_RIGHT_21=1112,23
PRIMER_LEFT_21_TM=59.988
PRIMER_RIGHT_21_TM=60.746
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=47.826
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=609
PRIMER_PAIR_22_PENALTY=3.758362
PRIMER_LEFT_22_PENALTY=0.012411
PRIMER_RIGHT_22_PENALTY=3.745951
PRIMER_LEFT_22_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_22_SEQUENCE=AGGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_22=504,20
PRIMER_RIGHT_22=1113,23
PRIMER_LEFT_22_TM=59.988
PRIMER_RIGHT_22_TM=60.746
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=47.826
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=610
PRIMER_PAIR_23_PENALTY=3.804724
PRIMER_LEFT_23_PENALTY=1.722217
PRIMER_RIGHT_23_PENALTY=2.082508
PRIMER_LEFT_23_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_23_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_23=374,20
PRIMER_RIGHT_23=1112,22
PRIMER_LEFT_23_TM=58.278
PRIMER_RIGHT_23_TM=59.917
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=739
PRIMER_PAIR_24_PENALTY=3.831805
PRIMER_LEFT_24_PENALTY=2.500551
PRIMER_RIGHT_24_PENALTY=1.331254
PRIMER_LEFT_24_SEQUENCE=CTTGTGAAGGAAATCTTGGGTT
PRIMER_RIGHT_24_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_24=662,22
PRIMER_RIGHT_24=1114,21
PRIMER_LEFT_24_TM=59.499
PRIMER_RIGHT_24_TM=59.669
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=7.1000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=453
PRIMER_PAIR_25_PENALTY=3.852531
PRIMER_LEFT_25_PENALTY=1.770024
PRIMER_RIGHT_25_PENALTY=2.082508
PRIMER_LEFT_25_SEQUENCE=TCATTGCCTCCAAGTCATCT
PRIMER_RIGHT_25_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_25=383,20
PRIMER_RIGHT_25=1112,22
PRIMER_LEFT_25_TM=58.230
PRIMER_RIGHT_25_TM=59.917
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=730
PRIMER_PAIR_26_PENALTY=3.875336
PRIMER_LEFT_26_PENALTY=2.544082
PRIMER_RIGHT_26_PENALTY=1.331254
PRIMER_LEFT_26_SEQUENCE=GCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_26_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_26=377,20
PRIMER_RIGHT_26=1114,21
PRIMER_LEFT_26_TM=57.456
PRIMER_RIGHT_26_TM=59.669
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=7.1000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=738
PRIMER_PAIR_27_PENALTY=3.913565
PRIMER_LEFT_27_PENALTY=1.831057
PRIMER_RIGHT_27_PENALTY=2.082508
PRIMER_LEFT_27_SEQUENCE=CCTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_27_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_27=661,21
PRIMER_RIGHT_27=1112,22
PRIMER_LEFT_27_TM=60.831
PRIMER_RIGHT_27_TM=59.917
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=10.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=452
PRIMER_PAIR_28_PENALTY=3.990140
PRIMER_LEFT_28_PENALTY=2.658886
PRIMER_RIGHT_28_PENALTY=1.331254
PRIMER_LEFT_28_SEQUENCE=GCTTTATCATTGCCTCCAAGT
PRIMER_RIGHT_28_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_28=377,21
PRIMER_RIGHT_28=1114,21
PRIMER_LEFT_28_TM=58.341
PRIMER_RIGHT_28_TM=59.669
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=738
PRIMER_PAIR_29_PENALTY=3.993317
PRIMER_LEFT_29_PENALTY=2.662063
PRIMER_RIGHT_29_PENALTY=1.331254
PRIMER_LEFT_29_SEQUENCE=CTTCATGGGCAGCAACCTCT
PRIMER_RIGHT_29_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_29=691,20
PRIMER_RIGHT_29=1114,21
PRIMER_LEFT_29_TM=62.662
PRIMER_RIGHT_29_TM=59.669
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=7.1000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=424
PRIMER_PAIR_30_PENALTY=4.017198
PRIMER_LEFT_30_PENALTY=1.934690
PRIMER_RIGHT_30_PENALTY=2.082508
PRIMER_LEFT_30_SEQUENCE=GGCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_30_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_30=376,21
PRIMER_RIGHT_30=1112,22
PRIMER_LEFT_30_TM=60.935
PRIMER_RIGHT_30_TM=59.917
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=737
PRIMER_PAIR_31_PENALTY=4.017198
PRIMER_LEFT_31_PENALTY=1.934690
PRIMER_RIGHT_31_PENALTY=2.082508
PRIMER_LEFT_31_SEQUENCE=AAGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_31_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_31=374,21
PRIMER_RIGHT_31=1112,22
PRIMER_LEFT_31_TM=60.935
PRIMER_RIGHT_31_TM=59.917
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=739
PRIMER_PAIR_32_PENALTY=4.077979
PRIMER_LEFT_32_PENALTY=0.332028
PRIMER_RIGHT_32_PENALTY=3.745951
PRIMER_LEFT_32_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_32_SEQUENCE=GGGGAAGATGGGTTATAGGTTCT
PRIMER_LEFT_32=375,20
PRIMER_RIGHT_32=1112,23
PRIMER_LEFT_32_TM=59.668
PRIMER_RIGHT_32_TM=60.746
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.826
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=738
PRIMER_PAIR_33_PENALTY=4.077979
PRIMER_LEFT_33_PENALTY=0.332028
PRIMER_RIGHT_33_PENALTY=3.745951
PRIMER_LEFT_33_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_33_SEQUENCE=AGGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_33=375,20
PRIMER_RIGHT_33=1113,23
PRIMER_LEFT_33_TM=59.668
PRIMER_RIGHT_33_TM=60.746
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=47.826
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=739
PRIMER_PAIR_34_PENALTY=4.089047
PRIMER_LEFT_34_PENALTY=2.757793
PRIMER_RIGHT_34_PENALTY=1.331254
PRIMER_LEFT_34_SEQUENCE=CTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_34_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_34=662,20
PRIMER_RIGHT_34=1114,21
PRIMER_LEFT_34_TM=57.242
PRIMER_RIGHT_34_TM=59.669
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.0000
PRIMER_RIGHT_34_END_STABILITY=7.1000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=453
PRIMER_PAIR_35_PENALTY=4.089047
PRIMER_LEFT_35_PENALTY=2.757793
PRIMER_RIGHT_35_PENALTY=1.331254
PRIMER_LEFT_35_SEQUENCE=CCTTGTGAAGGAAATCTTGG
PRIMER_RIGHT_35_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_35=661,20
PRIMER_RIGHT_35=1114,21
PRIMER_LEFT_35_TM=57.242
PRIMER_RIGHT_35_TM=59.669
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=7.1000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=454
PRIMER_PAIR_36_PENALTY=4.149476
PRIMER_LEFT_36_PENALTY=1.722217
PRIMER_RIGHT_36_PENALTY=2.427259
PRIMER_LEFT_36_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_36_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_36=374,20
PRIMER_RIGHT_36=1114,22
PRIMER_LEFT_36_TM=58.278
PRIMER_RIGHT_36_TM=60.427
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=741
PRIMER_PAIR_37_PENALTY=4.170222
PRIMER_LEFT_37_PENALTY=2.838968
PRIMER_RIGHT_37_PENALTY=1.331254
PRIMER_LEFT_37_SEQUENCE=CTTGTGAAGGAAATCTTGGGT
PRIMER_RIGHT_37_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_37=662,21
PRIMER_RIGHT_37=1114,21
PRIMER_LEFT_37_TM=58.161
PRIMER_RIGHT_37_TM=59.669
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=7.1000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=453
PRIMER_PAIR_38_PENALTY=4.170222
PRIMER_LEFT_38_PENALTY=2.838968
PRIMER_RIGHT_38_PENALTY=1.331254
PRIMER_LEFT_38_SEQUENCE=ACCTTGTGAAGGAAATCTTGG
PRIMER_RIGHT_38_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_38=660,21
PRIMER_RIGHT_38=1114,21
PRIMER_LEFT_38_TM=58.161
PRIMER_RIGHT_38_TM=59.669
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=7.1000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=455
PRIMER_PAIR_39_PENALTY=4.184784
PRIMER_LEFT_39_PENALTY=2.102276
PRIMER_RIGHT_39_PENALTY=2.082508
PRIMER_LEFT_39_SEQUENCE=GCTTTATCATTGCCTCCAAGTC
PRIMER_RIGHT_39_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_39=377,22
PRIMER_RIGHT_39=1112,22
PRIMER_LEFT_39_TM=60.102
PRIMER_RIGHT_39_TM=59.917
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=736
PRIMER_PAIR_40_PENALTY=4.248738
PRIMER_LEFT_40_PENALTY=2.917484
PRIMER_RIGHT_40_PENALTY=1.331254
PRIMER_LEFT_40_SEQUENCE=TGTGAAGGAAATCTTGGGTT
PRIMER_RIGHT_40_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_40=664,20
PRIMER_RIGHT_40=1114,21
PRIMER_LEFT_40_TM=57.083
PRIMER_RIGHT_40_TM=59.669
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.1000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=451
PRIMER_PAIR_41_PENALTY=4.248738
PRIMER_LEFT_41_PENALTY=2.917484
PRIMER_RIGHT_41_PENALTY=1.331254
PRIMER_LEFT_41_SEQUENCE=TTGTGAAGGAAATCTTGGGT
PRIMER_RIGHT_41_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_41=663,20
PRIMER_RIGHT_41=1114,21
PRIMER_LEFT_41_TM=57.083
PRIMER_RIGHT_41_TM=59.669
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=7.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=452
PRIMER_PAIR_42_PENALTY=4.258316
PRIMER_LEFT_42_PENALTY=1.831057
PRIMER_RIGHT_42_PENALTY=2.427259
PRIMER_LEFT_42_SEQUENCE=CCTTGTGAAGGAAATCTTGGG
PRIMER_RIGHT_42_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_42=661,21
PRIMER_RIGHT_42=1114,22
PRIMER_LEFT_42_TM=60.831
PRIMER_RIGHT_42_TM=60.427
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.0000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=454
PRIMER_PAIR_43_PENALTY=4.353137
PRIMER_LEFT_43_PENALTY=3.021883
PRIMER_RIGHT_43_PENALTY=1.331254
PRIMER_LEFT_43_SEQUENCE=ATGGGCAGCAACCTCTGAAA
PRIMER_RIGHT_43_SEQUENCE=CAGGGGAAGATGGGTTATAGG
PRIMER_LEFT_43=695,20
PRIMER_RIGHT_43=1114,21
PRIMER_LEFT_43_TM=63.022
PRIMER_RIGHT_43_TM=59.669
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=7.1000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=420
PRIMER_PAIR_44_PENALTY=4.361949
PRIMER_LEFT_44_PENALTY=1.934690
PRIMER_RIGHT_44_PENALTY=2.427259
PRIMER_LEFT_44_SEQUENCE=GGCTTTATCATTGCCTCCAAG
PRIMER_RIGHT_44_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_44=376,21
PRIMER_RIGHT_44=1114,22
PRIMER_LEFT_44_TM=60.935
PRIMER_RIGHT_44_TM=60.427
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=739
PRIMER_PAIR_45_PENALTY=4.361949
PRIMER_LEFT_45_PENALTY=1.934690
PRIMER_RIGHT_45_PENALTY=2.427259
PRIMER_LEFT_45_SEQUENCE=AAGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_45_SEQUENCE=CAGGGGAAGATGGGTTATAGGT
PRIMER_LEFT_45=374,21
PRIMER_RIGHT_45=1114,22
PRIMER_LEFT_45_TM=60.935
PRIMER_RIGHT_45_TM=60.427
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=741
PRIMER_PAIR_46_PENALTY=4.456579
PRIMER_LEFT_46_PENALTY=0.012411
PRIMER_RIGHT_46_PENALTY=4.444168
PRIMER_LEFT_46_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_46_SEQUENCE=GGGAAGATGGGTTATAGGTTCT
PRIMER_LEFT_46=504,20
PRIMER_RIGHT_46=1111,22
PRIMER_LEFT_46_TM=59.988
PRIMER_RIGHT_46_TM=57.556
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=608
PRIMER_PAIR_47_PENALTY=4.473129
PRIMER_LEFT_47_PENALTY=2.390622
PRIMER_RIGHT_47_PENALTY=2.082508
PRIMER_LEFT_47_SEQUENCE=ATCATTGCCTCCAAGTCATCT
PRIMER_RIGHT_47_SEQUENCE=GGGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_47=382,21
PRIMER_RIGHT_47=1112,22
PRIMER_LEFT_47_TM=58.609
PRIMER_RIGHT_47_TM=59.917
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=731
PRIMER_PAIR_48_PENALTY=4.475882
PRIMER_LEFT_48_PENALTY=0.012411
PRIMER_RIGHT_48_PENALTY=4.463471
PRIMER_LEFT_48_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_48_SEQUENCE=GGGAAGATGGGTTATAGGTTC
PRIMER_LEFT_48=504,20
PRIMER_RIGHT_48=1111,21
PRIMER_LEFT_48_TM=59.988
PRIMER_RIGHT_48_TM=56.537
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=608
PRIMER_PAIR_49_PENALTY=4.509740
PRIMER_LEFT_49_PENALTY=1.722217
PRIMER_RIGHT_49_PENALTY=2.787523
PRIMER_LEFT_49_SEQUENCE=AAGGCTTTATCATTGCCTCC
PRIMER_RIGHT_49_SEQUENCE=GGGGAAGATGGGTTATAGGTT
PRIMER_LEFT_49=374,20
PRIMER_RIGHT_49=1112,21
PRIMER_LEFT_49_TM=58.278
PRIMER_RIGHT_49_TM=58.212
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=739
=
SEQUENCE_ID=MAGIC.433_4_8110663_8110686_ct-1_wu-0_57993_176070_TRUE
SEQUENCE_TARGET=950,123
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTCTATTGANAAGGCNAGTTGTTGTTGCTTGNATANCTTTCATTCNGGATACCANTNGNGCNATGCCNNTTNGGNNTNNNNNTCTCCCCAANNNNNNNNNNNNNNNNNAGTTGTTGTTGCTTGNATAGCTTTCATTCTGGATACCANTNGTGCNATGCCTGCCCTNNNATNCANANNNNNNNNNNNTTGTGAAAAAGACCATTTCTNCCNCAAACTNAACNACNCATTNCTTTATCTGANTAAGGAAAATGTTCATCCNATCATGATCATTTGACTGCGTAGAGGTCTCNGAATCATCGAGACAAAACCCGCTTNCATCATCTTNNGCCANCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTCTCCNANCTCGAGAGATATGNAACNTGAATCANAGTTGTAAAGANCNCAATTGTNAGGTGTTNAGACNTTTTTTGTTTTTNTNTAAATAGAANNNNNNNNNNNNNAAATTTNANAACGTTTAGGANTNGCAAAAATCTTGTGATACNNTGTACATANANTTTNATTTAATGTGATGANCTGTGATTTANNTTTTTNTTTTTATNTNAGTTGAAATTTANCTTTTGGAGAATATCNTAAATNCCATANTTCNANTAAATNATAGAAANATAAAGANTTTTTGTTGAAGATTNAAAGAAACTTTGACCAAATNTATNTAGAAGAGATNTTTNACCNTTTTAAACAATGAGATTATATCNTCCATAAACATNNNNNNNNNNNNNNNNAANNGGATTTCTTACCCAATNCTNCTTATTTATTTTCNAACTTAACATTTTAGGCCTTGANACAATATNAGAATCNGANATGACCAANACCAAACCANACTGNCCNGTTNACCANANNNNNNNNNNNNNNNNNNNNNNNNNNANGGTTGCTTACTCATATGCAAATCAACCCTAAGANNNNNNNNNNNNNAGGAGAAAANACAAATCAAAGATGGTCCTNGNCGACTCACCCGGACCACACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNAACNAAATTGAAGATTCATCAACAAACNACACATTTTAAGNTCGNTTGATCAANGAATAATCATAAANNAANGAGCNAGTCCACTAGAATGCCNTGATNACCCACNTNNATGAAGCCANTTTCACNTTCATCACCCCAAAGNCAACTTTCATCGNCCCATATTCGACATTNANTGATATNATNNNNNTTTATGAAGAATGGGCNAGTCATTNNNNNNNNNNACCGAACCATTCANCTAGTTNTTANGNGANCCTTCCCGAATCGTACNAAGTTNAGTTTCNNCAATCAAATGACANATTTCCTAGAACNNTATTGAAATTTCACCAACCAACAACCTATTTTATGAAGAATTGGTGAGGAANATTTGTGTGTTATCAAGGTATATCGAAGATGGTCGAGAGACACTCAATTCAGACTATACGAGCACCTACCTAGAGCTGTTTAAGGAAAGTTNTTTTTTTCTTTCCTTTGTATGCATCTCAAAGCCACATATGGAAACTAAGAGATCTTGTACACATTCTAGAAGCTTNTCCACACGTCCAGTGGTNCNCTTTCCCCACTTGATCATGCAAAGANCAAACTTGCCATATTTGGANCTNGAANGTTTAATTCACAAAAACTCTTTTCTTGTCACTGCTGCTGNGTTTTTAAGGCCCTCCNCATGTCCCACAATTTCCCAAGATANTACANAANT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_5198629_5198712_po-0_can-0_12947_12718_TRUE
SEQUENCE_TARGET=950,183
SEQUENCE_TEMPLATE=GCCATTTTCATATCANCNCTGTAAACTTGNCACCAATGCATTCAGCACATCATCTTTGAAACTTGTAAGAGGAGAAGNAGTGGTAATCAATTGTATGTTGCATCTTCCTCGGTTTAGTCACCAAACACCCANTTCNGTCATTTCCTTTTTGTCGGAAGCTAAAACTCTAAACCCNAAACTTGTTACNCTTGTTCACGAAGAAGTTGGTCTGATGGGNAACCAAGGTTTTCTATATNGTTTTATGGACTTGCTACATCAGTTCTCNGCCATATTCGACTCTCTAGAGGCAGGCCTTTCAATNNCNAACCCGGCCCGTGGCTTTGTTGAGCGTGTTTTTATCGGTCCTTGGGTTGCNNATTGGTTGANGCGTATCACCGCTNATGATGCAGAAGTCGAGAGCTNTGCTTCGTGGCNACAGTGGTTAGAGACTAATGGGTTCAAACCCTTGGAAGTGAGCTTTNCCAACCGTTGTCAAGCAAAACTCCTTTTGAGTTTGTTTAANGATNGATTTAGAGTGGAAGAATTGGGCCAANACGGNCTCGTTTTGGGATGGAAATCTCGGCGTCTTGTNTNNGCCTCCTTTTGGGCCTCGTGCNAGACGAATCAGTAAATTCTCGTTTNTGCATTCTCGAGTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTATGTGAATATCGAACGTGTAATTTCTCAAAAANGACAGTTTAGTTTAATATGANAAGTNATTGAGCCACGACATTGCATTGTATGTTGAATCTTTAATCNTNTATATAAATTANAATGANNAACTCGACANTCGTTTTACACAAGACTATGTTTGNANATACGTGTNAATATGTTGTAGTAATTTTGGTAGCGGACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNGCCATACTTGTAGCCGCAAACANCATAAATAATATTTTTGGTCAAAGTTTCATCATTGGAATTGGTAACTCTCTTANTTGAANTGNTGGAANTAGGGTTCCAACCNTCCTCTTTTCATAAAGCTTGTTTAATTTATTTATTTTANNTGNTTGCAGNCTANAATANNNTNAGCTGCATAGAAGNGGANACGGAAANGCTCNGAATTNCTCGAAATCGTCTCAGAATCGCGANTNCTAAAATGATGNGTTTGGANACGTGGANGAANCGGACAATTCAGCTTTNGAANCGTCGGTGGTTGAGATCGANAATGTCTCTACCGTAAATCNAAGATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.089485
PRIMER_LEFT_0_PENALTY=0.205314
PRIMER_RIGHT_0_PENALTY=0.884171
PRIMER_LEFT_0_SEQUENCE=TTTTATCGGTCCTTGGGTTG
PRIMER_RIGHT_0_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_0=333,20
PRIMER_RIGHT_0=1153,20
PRIMER_LEFT_0_TM=59.795
PRIMER_RIGHT_0_TM=60.884
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=821
PRIMER_PAIR_1_PENALTY=1.089485
PRIMER_LEFT_1_PENALTY=0.205314
PRIMER_RIGHT_1_PENALTY=0.884171
PRIMER_LEFT_1_SEQUENCE=TGTTTTTATCGGTCCTTGGG
PRIMER_RIGHT_1_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_1=330,20
PRIMER_RIGHT_1=1153,20
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=60.884
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=824
PRIMER_PAIR_2_PENALTY=1.175840
PRIMER_LEFT_2_PENALTY=0.291669
PRIMER_RIGHT_2_PENALTY=0.884171
PRIMER_LEFT_2_SEQUENCE=GGCTTTGTTGAGCGTGTTTT
PRIMER_RIGHT_2_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_2=316,20
PRIMER_RIGHT_2=1153,20
PRIMER_LEFT_2_TM=60.292
PRIMER_RIGHT_2_TM=60.884
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=838
PRIMER_PAIR_3_PENALTY=1.552486
PRIMER_LEFT_3_PENALTY=0.668315
PRIMER_RIGHT_3_PENALTY=0.884171
PRIMER_LEFT_3_SEQUENCE=CCGTTGTCAAGCAAAACTCC
PRIMER_RIGHT_3_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_3=465,20
PRIMER_RIGHT_3=1153,20
PRIMER_LEFT_3_TM=60.668
PRIMER_RIGHT_3_TM=60.884
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=689
PRIMER_PAIR_4_PENALTY=1.793406
PRIMER_LEFT_4_PENALTY=0.909234
PRIMER_RIGHT_4_PENALTY=0.884171
PRIMER_LEFT_4_SEQUENCE=GCGTGTTTTTATCGGTCCTT
PRIMER_RIGHT_4_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_4=327,20
PRIMER_RIGHT_4=1153,20
PRIMER_LEFT_4_TM=59.091
PRIMER_RIGHT_4_TM=60.884
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=827
PRIMER_PAIR_5_PENALTY=1.898392
PRIMER_LEFT_5_PENALTY=1.014220
PRIMER_RIGHT_5_PENALTY=0.884171
PRIMER_LEFT_5_SEQUENCE=AGCGTGTTTTTATCGGTCCTT
PRIMER_RIGHT_5_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_5=326,21
PRIMER_RIGHT_5=1153,20
PRIMER_LEFT_5_TM=60.014
PRIMER_RIGHT_5_TM=60.884
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=828
PRIMER_PAIR_6_PENALTY=2.008096
PRIMER_LEFT_6_PENALTY=1.123924
PRIMER_RIGHT_6_PENALTY=0.884171
PRIMER_LEFT_6_SEQUENCE=TTGAGCGTGTTTTTATCGGTC
PRIMER_RIGHT_6_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_6=323,21
PRIMER_RIGHT_6=1153,20
PRIMER_LEFT_6_TM=60.124
PRIMER_RIGHT_6_TM=60.884
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=831
PRIMER_PAIR_7_PENALTY=2.111073
PRIMER_LEFT_7_PENALTY=1.226901
PRIMER_RIGHT_7_PENALTY=0.884171
PRIMER_LEFT_7_SEQUENCE=TGAGCGTGTTTTTATCGGTC
PRIMER_RIGHT_7_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_7=324,20
PRIMER_RIGHT_7=1153,20
PRIMER_LEFT_7_TM=58.773
PRIMER_RIGHT_7_TM=60.884
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=830
PRIMER_PAIR_8_PENALTY=2.159508
PRIMER_LEFT_8_PENALTY=1.275336
PRIMER_RIGHT_8_PENALTY=0.884171
PRIMER_LEFT_8_SEQUENCE=GTGGCTTTGTTGAGCGTGTT
PRIMER_RIGHT_8_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_8=314,20
PRIMER_RIGHT_8=1153,20
PRIMER_LEFT_8_TM=61.275
PRIMER_RIGHT_8_TM=60.884
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=840
PRIMER_PAIR_9_PENALTY=2.163925
PRIMER_LEFT_9_PENALTY=1.279754
PRIMER_RIGHT_9_PENALTY=0.884171
PRIMER_LEFT_9_SEQUENCE=AGCGTGTTTTTATCGGTCCT
PRIMER_RIGHT_9_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_9=326,20
PRIMER_RIGHT_9=1153,20
PRIMER_LEFT_9_TM=58.720
PRIMER_RIGHT_9_TM=60.884
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=828
PRIMER_PAIR_10_PENALTY=2.375256
PRIMER_LEFT_10_PENALTY=1.491085
PRIMER_RIGHT_10_PENALTY=0.884171
PRIMER_LEFT_10_SEQUENCE=GATGGAAATCTCGGCGTCTT
PRIMER_RIGHT_10_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_10=548,20
PRIMER_RIGHT_10=1153,20
PRIMER_LEFT_10_TM=61.491
PRIMER_RIGHT_10_TM=60.884
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=606
PRIMER_PAIR_11_PENALTY=2.381047
PRIMER_LEFT_11_PENALTY=1.496875
PRIMER_RIGHT_11_PENALTY=0.884171
PRIMER_LEFT_11_SEQUENCE=GAGCGTGTTTTTATCGGTCCT
PRIMER_RIGHT_11_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_11=325,21
PRIMER_RIGHT_11=1153,20
PRIMER_LEFT_11_TM=60.497
PRIMER_RIGHT_11_TM=60.884
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=829
PRIMER_PAIR_12_PENALTY=2.462729
PRIMER_LEFT_12_PENALTY=1.578557
PRIMER_RIGHT_12_PENALTY=0.884171
PRIMER_LEFT_12_SEQUENCE=TGTTTTTATCGGTCCTTGGGT
PRIMER_RIGHT_12_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_12=330,21
PRIMER_RIGHT_12=1153,20
PRIMER_LEFT_12_TM=60.579
PRIMER_RIGHT_12_TM=60.884
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=824
PRIMER_PAIR_13_PENALTY=2.462729
PRIMER_LEFT_13_PENALTY=1.578557
PRIMER_RIGHT_13_PENALTY=0.884171
PRIMER_LEFT_13_SEQUENCE=GTGTTTTTATCGGTCCTTGGG
PRIMER_RIGHT_13_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_13=329,21
PRIMER_RIGHT_13=1153,20
PRIMER_LEFT_13_TM=60.579
PRIMER_RIGHT_13_TM=60.884
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=825
PRIMER_PAIR_14_PENALTY=2.467051
PRIMER_LEFT_14_PENALTY=1.582879
PRIMER_RIGHT_14_PENALTY=0.884171
PRIMER_LEFT_14_SEQUENCE=TTTTTATCGGTCCTTGGGTT
PRIMER_RIGHT_14_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_14=332,20
PRIMER_RIGHT_14=1153,20
PRIMER_LEFT_14_TM=58.417
PRIMER_RIGHT_14_TM=60.884
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=822
PRIMER_PAIR_15_PENALTY=2.590453
PRIMER_LEFT_15_PENALTY=1.706282
PRIMER_RIGHT_15_PENALTY=0.884171
PRIMER_LEFT_15_SEQUENCE=GTTGAGCGTGTTTTTATCGG
PRIMER_RIGHT_15_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_15=322,20
PRIMER_RIGHT_15=1153,20
PRIMER_LEFT_15_TM=58.294
PRIMER_RIGHT_15_TM=60.884
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.8000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=832
PRIMER_PAIR_16_PENALTY=2.597786
PRIMER_LEFT_16_PENALTY=1.713615
PRIMER_RIGHT_16_PENALTY=0.884171
PRIMER_LEFT_16_SEQUENCE=CGAAGAAGTTGGTCTGATGG
PRIMER_RIGHT_16_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_16=195,20
PRIMER_RIGHT_16=1153,20
PRIMER_LEFT_16_TM=58.286
PRIMER_RIGHT_16_TM=60.884
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=959
PRIMER_PAIR_17_PENALTY=2.608332
PRIMER_LEFT_17_PENALTY=1.724161
PRIMER_RIGHT_17_PENALTY=0.884171
PRIMER_LEFT_17_SEQUENCE=AACCGTTGTCAAGCAAAACTC
PRIMER_RIGHT_17_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_17=463,21
PRIMER_RIGHT_17=1153,20
PRIMER_LEFT_17_TM=59.276
PRIMER_RIGHT_17_TM=60.884
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=691
PRIMER_PAIR_18_PENALTY=2.651562
PRIMER_LEFT_18_PENALTY=1.767391
PRIMER_RIGHT_18_PENALTY=0.884171
PRIMER_LEFT_18_SEQUENCE=GTTTTTATCGGTCCTTGGGTT
PRIMER_RIGHT_18_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_18=331,21
PRIMER_RIGHT_18=1153,20
PRIMER_LEFT_18_TM=59.233
PRIMER_RIGHT_18_TM=60.884
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=823
PRIMER_PAIR_19_PENALTY=2.654824
PRIMER_LEFT_19_PENALTY=1.770653
PRIMER_RIGHT_19_PENALTY=0.884171
PRIMER_LEFT_19_SEQUENCE=TGGCTTTGTTGAGCGTGTTT
PRIMER_RIGHT_19_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_19=315,20
PRIMER_RIGHT_19=1153,20
PRIMER_LEFT_19_TM=61.771
PRIMER_RIGHT_19_TM=60.884
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=839
PRIMER_PAIR_20_PENALTY=2.686766
PRIMER_LEFT_20_PENALTY=1.802595
PRIMER_RIGHT_20_PENALTY=0.884171
PRIMER_LEFT_20_SEQUENCE=ACGAAGAAGTTGGTCTGATGG
PRIMER_RIGHT_20_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_20=194,21
PRIMER_RIGHT_20=1153,20
PRIMER_LEFT_20_TM=59.197
PRIMER_RIGHT_20_TM=60.884
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=960
PRIMER_PAIR_21_PENALTY=2.736805
PRIMER_LEFT_21_PENALTY=1.852633
PRIMER_RIGHT_21_PENALTY=0.884171
PRIMER_LEFT_21_SEQUENCE=CGTGTTTTTATCGGTCCTTG
PRIMER_RIGHT_21_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_21=328,20
PRIMER_RIGHT_21=1153,20
PRIMER_LEFT_21_TM=58.147
PRIMER_RIGHT_21_TM=60.884
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=826
PRIMER_PAIR_22_PENALTY=2.803389
PRIMER_LEFT_22_PENALTY=1.919218
PRIMER_RIGHT_22_PENALTY=0.884171
PRIMER_LEFT_22_SEQUENCE=GCTTTGTTGAGCGTGTTTTT
PRIMER_RIGHT_22_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_22=317,20
PRIMER_RIGHT_22=1153,20
PRIMER_LEFT_22_TM=58.081
PRIMER_RIGHT_22_TM=60.884
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=837
PRIMER_PAIR_23_PENALTY=2.875752
PRIMER_LEFT_23_PENALTY=1.991580
PRIMER_RIGHT_23_PENALTY=0.884171
PRIMER_LEFT_23_SEQUENCE=CGTTGTCAAGCAAAACTCCT
PRIMER_RIGHT_23_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_23=466,20
PRIMER_RIGHT_23=1153,20
PRIMER_LEFT_23_TM=58.008
PRIMER_RIGHT_23_TM=60.884
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=688
PRIMER_PAIR_24_PENALTY=2.915501
PRIMER_LEFT_24_PENALTY=2.031330
PRIMER_RIGHT_24_PENALTY=0.884171
PRIMER_LEFT_24_SEQUENCE=TGTTGAGCGTGTTTTTATCGG
PRIMER_RIGHT_24_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_24=321,21
PRIMER_RIGHT_24=1153,20
PRIMER_LEFT_24_TM=61.031
PRIMER_RIGHT_24_TM=60.884
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.8000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=833
PRIMER_PAIR_25_PENALTY=2.925270
PRIMER_LEFT_25_PENALTY=2.041099
PRIMER_RIGHT_25_PENALTY=0.884171
PRIMER_LEFT_25_SEQUENCE=TTTTTATCGGTCCTTGGGTTG
PRIMER_RIGHT_25_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_25=332,21
PRIMER_RIGHT_25=1153,20
PRIMER_LEFT_25_TM=61.041
PRIMER_RIGHT_25_TM=60.884
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=822
PRIMER_PAIR_26_PENALTY=2.931568
PRIMER_LEFT_26_PENALTY=2.047396
PRIMER_RIGHT_26_PENALTY=0.884171
PRIMER_LEFT_26_SEQUENCE=TTTTGGGATGGAAATCTCGG
PRIMER_RIGHT_26_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_26=542,20
PRIMER_RIGHT_26=1153,20
PRIMER_LEFT_26_TM=62.047
PRIMER_RIGHT_26_TM=60.884
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=612
PRIMER_PAIR_27_PENALTY=2.975037
PRIMER_LEFT_27_PENALTY=2.090865
PRIMER_RIGHT_27_PENALTY=0.884171
PRIMER_LEFT_27_SEQUENCE=AACCCTTGGAAGTGAGCTTT
PRIMER_RIGHT_27_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_27=440,20
PRIMER_RIGHT_27=1153,20
PRIMER_LEFT_27_TM=57.909
PRIMER_RIGHT_27_TM=60.884
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=714
PRIMER_PAIR_28_PENALTY=2.981395
PRIMER_LEFT_28_PENALTY=2.097224
PRIMER_RIGHT_28_PENALTY=0.884171
PRIMER_LEFT_28_SEQUENCE=GTTTTTATCGGTCCTTGGGT
PRIMER_RIGHT_28_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_28=331,20
PRIMER_RIGHT_28=1153,20
PRIMER_LEFT_28_TM=57.903
PRIMER_RIGHT_28_TM=60.884
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=823
PRIMER_PAIR_29_PENALTY=3.029690
PRIMER_LEFT_29_PENALTY=2.145518
PRIMER_RIGHT_29_PENALTY=0.884171
PRIMER_LEFT_29_SEQUENCE=ACCGTTGTCAAGCAAAACTC
PRIMER_RIGHT_29_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_29=464,20
PRIMER_RIGHT_29=1153,20
PRIMER_LEFT_29_TM=57.854
PRIMER_RIGHT_29_TM=60.884
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=690
PRIMER_PAIR_30_PENALTY=3.114420
PRIMER_LEFT_30_PENALTY=2.230249
PRIMER_RIGHT_30_PENALTY=0.884171
PRIMER_LEFT_30_SEQUENCE=TTCAAACCCTTGGAAGTGAG
PRIMER_RIGHT_30_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_30=436,20
PRIMER_RIGHT_30=1153,20
PRIMER_LEFT_30_TM=57.770
PRIMER_RIGHT_30_TM=60.884
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=718
PRIMER_PAIR_31_PENALTY=3.202550
PRIMER_LEFT_31_PENALTY=2.318379
PRIMER_RIGHT_31_PENALTY=0.884171
PRIMER_LEFT_31_SEQUENCE=GTTCAAACCCTTGGAAGTGAG
PRIMER_RIGHT_31_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_31=435,21
PRIMER_RIGHT_31=1153,20
PRIMER_LEFT_31_TM=58.682
PRIMER_RIGHT_31_TM=60.884
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=719
PRIMER_PAIR_32_PENALTY=3.313537
PRIMER_LEFT_32_PENALTY=2.429366
PRIMER_RIGHT_32_PENALTY=0.884171
PRIMER_LEFT_32_SEQUENCE=ATGGAAATCTCGGCGTCTTG
PRIMER_RIGHT_32_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_32=549,20
PRIMER_RIGHT_32=1153,20
PRIMER_LEFT_32_TM=62.429
PRIMER_RIGHT_32_TM=60.884
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=605
PRIMER_PAIR_33_PENALTY=3.331178
PRIMER_LEFT_33_PENALTY=2.447006
PRIMER_RIGHT_33_PENALTY=0.884171
PRIMER_LEFT_33_SEQUENCE=ACCGTTGTCAAGCAAAACTCC
PRIMER_RIGHT_33_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_33=464,21
PRIMER_RIGHT_33=1153,20
PRIMER_LEFT_33_TM=61.447
PRIMER_RIGHT_33_TM=60.884
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.6000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=690
PRIMER_PAIR_34_PENALTY=3.411957
PRIMER_LEFT_34_PENALTY=2.527786
PRIMER_RIGHT_34_PENALTY=0.884171
PRIMER_LEFT_34_SEQUENCE=GGCTTTGTTGAGCGTGTTTTT
PRIMER_RIGHT_34_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_34=316,21
PRIMER_RIGHT_34=1153,20
PRIMER_LEFT_34_TM=61.528
PRIMER_RIGHT_34_TM=60.884
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.6000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=838
PRIMER_PAIR_35_PENALTY=3.448454
PRIMER_LEFT_35_PENALTY=2.564282
PRIMER_RIGHT_35_PENALTY=0.884171
PRIMER_LEFT_35_SEQUENCE=CCGTTGTCAAGCAAAACTCCT
PRIMER_RIGHT_35_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_35=465,21
PRIMER_RIGHT_35=1153,20
PRIMER_LEFT_35_TM=61.564
PRIMER_RIGHT_35_TM=60.884
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=689
PRIMER_PAIR_36_PENALTY=3.494723
PRIMER_LEFT_36_PENALTY=2.610551
PRIMER_RIGHT_36_PENALTY=0.884171
PRIMER_LEFT_36_SEQUENCE=AACCGTTGTCAAGCAAAACT
PRIMER_RIGHT_36_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_36=463,20
PRIMER_RIGHT_36=1153,20
PRIMER_LEFT_36_TM=57.389
PRIMER_RIGHT_36_TM=60.884
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=691
PRIMER_PAIR_37_PENALTY=3.610605
PRIMER_LEFT_37_PENALTY=2.726433
PRIMER_RIGHT_37_PENALTY=0.884171
PRIMER_LEFT_37_SEQUENCE=GCGTGTTTTTATCGGTCCTTG
PRIMER_RIGHT_37_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_37=327,21
PRIMER_RIGHT_37=1153,20
PRIMER_LEFT_37_TM=61.726
PRIMER_RIGHT_37_TM=60.884
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=827
PRIMER_PAIR_38_PENALTY=3.752994
PRIMER_LEFT_38_PENALTY=2.868823
PRIMER_RIGHT_38_PENALTY=0.884171
PRIMER_LEFT_38_SEQUENCE=GAAGAAGTTGGTCTGATGGG
PRIMER_RIGHT_38_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_38=196,20
PRIMER_RIGHT_38=1153,20
PRIMER_LEFT_38_TM=57.131
PRIMER_RIGHT_38_TM=60.884
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=958
PRIMER_PAIR_39_PENALTY=3.770808
PRIMER_LEFT_39_PENALTY=2.886637
PRIMER_RIGHT_39_PENALTY=0.884171
PRIMER_LEFT_39_SEQUENCE=GTTGAGCGTGTTTTTATCGGTC
PRIMER_RIGHT_39_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_39=322,22
PRIMER_RIGHT_39=1153,20
PRIMER_LEFT_39_TM=60.887
PRIMER_RIGHT_39_TM=60.884
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=832
PRIMER_PAIR_40_PENALTY=3.826761
PRIMER_LEFT_40_PENALTY=2.942589
PRIMER_RIGHT_40_PENALTY=0.884171
PRIMER_LEFT_40_SEQUENCE=CGAAGAAGTTGGTCTGATGGG
PRIMER_RIGHT_40_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_40=195,21
PRIMER_RIGHT_40=1153,20
PRIMER_LEFT_40_TM=61.943
PRIMER_RIGHT_40_TM=60.884
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=959
PRIMER_PAIR_41_PENALTY=3.906054
PRIMER_LEFT_41_PENALTY=3.021883
PRIMER_RIGHT_41_PENALTY=0.884171
PRIMER_LEFT_41_SEQUENCE=TGGAAATCTCGGCGTCTTGT
PRIMER_RIGHT_41_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_41=550,20
PRIMER_RIGHT_41=1153,20
PRIMER_LEFT_41_TM=63.022
PRIMER_RIGHT_41_TM=60.884
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=604
PRIMER_PAIR_42_PENALTY=3.997270
PRIMER_LEFT_42_PENALTY=3.113098
PRIMER_RIGHT_42_PENALTY=0.884171
PRIMER_LEFT_42_SEQUENCE=TTGTTCACGAAGAAGTTGGTCT
PRIMER_RIGHT_42_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_42=188,22
PRIMER_RIGHT_42=1153,20
PRIMER_LEFT_42_TM=58.887
PRIMER_RIGHT_42_TM=60.884
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=966
PRIMER_PAIR_43_PENALTY=4.190650
PRIMER_LEFT_43_PENALTY=3.306479
PRIMER_RIGHT_43_PENALTY=0.884171
PRIMER_LEFT_43_SEQUENCE=GTGTTTTTATCGGTCCTTGGGT
PRIMER_RIGHT_43_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_43=329,22
PRIMER_RIGHT_43=1153,20
PRIMER_LEFT_43_TM=61.306
PRIMER_RIGHT_43_TM=60.884
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=825
PRIMER_PAIR_44_PENALTY=4.392063
PRIMER_LEFT_44_PENALTY=3.507892
PRIMER_RIGHT_44_PENALTY=0.884171
PRIMER_LEFT_44_SEQUENCE=GTGGCTTTGTTGAGCGTGTTT
PRIMER_RIGHT_44_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_44=314,21
PRIMER_RIGHT_44=1153,20
PRIMER_LEFT_44_TM=62.508
PRIMER_RIGHT_44_TM=60.884
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=840
PRIMER_PAIR_45_PENALTY=4.450541
PRIMER_LEFT_45_PENALTY=3.566369
PRIMER_RIGHT_45_PENALTY=0.884171
PRIMER_LEFT_45_SEQUENCE=TGTTCACGAAGAAGTTGGTCT
PRIMER_RIGHT_45_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_45=189,21
PRIMER_RIGHT_45=1153,20
PRIMER_LEFT_45_TM=57.434
PRIMER_RIGHT_45_TM=60.884
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=965
PRIMER_PAIR_46_PENALTY=4.552392
PRIMER_LEFT_46_PENALTY=3.668220
PRIMER_RIGHT_46_PENALTY=0.884171
PRIMER_LEFT_46_SEQUENCE=GAGCGTGTTTTTATCGGTCCTT
PRIMER_RIGHT_46_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_46=325,22
PRIMER_RIGHT_46=1153,20
PRIMER_LEFT_46_TM=61.668
PRIMER_RIGHT_46_TM=60.884
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=829
PRIMER_PAIR_47_PENALTY=4.567674
PRIMER_LEFT_47_PENALTY=3.683502
PRIMER_RIGHT_47_PENALTY=0.884171
PRIMER_LEFT_47_SEQUENCE=GGATGGAAATCTCGGCGTCT
PRIMER_RIGHT_47_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_47=547,20
PRIMER_RIGHT_47=1153,20
PRIMER_LEFT_47_TM=63.684
PRIMER_RIGHT_47_TM=60.884
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=607
PRIMER_PAIR_48_PENALTY=4.626986
PRIMER_LEFT_48_PENALTY=3.742815
PRIMER_RIGHT_48_PENALTY=0.884171
PRIMER_LEFT_48_SEQUENCE=GTTTTTATCGGTCCTTGGGTTG
PRIMER_RIGHT_48_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_48=331,22
PRIMER_RIGHT_48=1153,20
PRIMER_LEFT_48_TM=61.743
PRIMER_RIGHT_48_TM=60.884
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=823
PRIMER_PAIR_49_PENALTY=4.626986
PRIMER_LEFT_49_PENALTY=3.742815
PRIMER_RIGHT_49_PENALTY=0.884171
PRIMER_LEFT_49_SEQUENCE=TGTTTTTATCGGTCCTTGGGTT
PRIMER_RIGHT_49_SEQUENCE=TCGCGATTCTGAGACGATTT
PRIMER_LEFT_49=330,22
PRIMER_RIGHT_49=1153,20
PRIMER_LEFT_49_TM=61.743
PRIMER_RIGHT_49_TM=60.884
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=824
=
SEQUENCE_ID=MAGIC.175_2_4552009_4552076_oy-0_edi-0_3341_1381874_TRUE
SEQUENCE_TARGET=950,167
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_6483692_6483803_no-0_oy-0_12375_74257_TRUE
SEQUENCE_TARGET=950,211
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTTTGTTTTGCTCCACGACGANNAATCAANTATGTCTTCTTCGGCTTCTCTCGTATGTAAGGGTTTGATGTTACTTGTGTTTCATTTCTCCTCGTATGGCTTCCNTTCTCTTCCTTTTTATTCCATTTGGAAATCTGGACATCGACCTGAAATTTGTGCATGANTGNTNTGAGTGGANGAAATTTGTTCATTATTAGTATANAGANGATGTATATTTTTGAAGTTGTAACCAACNGATACATATTTCCANTTNCTTCCNAATNTTGTAAANCTAAACNTAGCCNCAAAAANCATGTTACTANATTNAGANTAANGAAACGAACAACAACCTGNGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANTTTNTTTTGTTTGCTCTATAANATTGACATGATAATAAAGTTAATTTGGTCTAGTNAAACAAATTAANGAGNTGGAGATTCAGTTTCCNATTAGGGAANCTCGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTAGACANTCGAACTAAGNTTCAACTAAATNTTCNAANAAAANAAAGATACTANAGAAACGCCNTTNANTTNNAAAANNNGGTAGGTGCATAGTAAATTGGTCAGCNATNCATGTGACAGTTATCGTGTAGANTCTATAGAGAGCCCTACATNAATGAAGCTTNTCGTGGCAAATTCTAGAAGTAGTATTTTAAGTCAGTCTAGNTTTGACCNATACATAGCTTATAACTAAAAGACTANANGTGTTTTCTCTNAATATACACTTTGTTATCAAANTACAAGTAATCAATCAAAAGATGGAAGNCAAAACTGTGATATTCAGTGTNCTCGTAATGGGTCTGGTCNTCTCGCAAATTCAAGTANAAGCNCAAAAAACTTGCTGCCCGAGCCAGTCAACTAGGAAGGAGTTTGAAGATTGCATTTCAGAAGGAAATTTGCAGATACTTTGTTCAGCTGAAAGTGGATGCCGAGACACTTACGTGGGTTATTGNCCTTCGGGNTTTCCTTACGGGAGTCTCACAAACTCTGGTAAAAACTGAACTCACTTGGTTATATGCTTTNTTAAGTTANATTTGTTAGANNATGGATCAAGATTTNATTTTTNATGCAAGGTGATGNTGTCAATGTATATTGTAAGTTNGGGTGTGTATCCTCTCTGTGTGGTGCCTTAACGAGTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.036635
PRIMER_LEFT_0_PENALTY=0.691563
PRIMER_RIGHT_0_PENALTY=3.345071
PRIMER_LEFT_0_SEQUENCE=TCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_0_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_0=604,20
PRIMER_RIGHT_0=1539,22
PRIMER_LEFT_0_TM=59.308
PRIMER_RIGHT_0_TM=58.655
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=936
PRIMER_PAIR_1_PENALTY=4.076537
PRIMER_LEFT_1_PENALTY=0.731465
PRIMER_RIGHT_1_PENALTY=3.345071
PRIMER_LEFT_1_SEQUENCE=TTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_1_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_1=654,20
PRIMER_RIGHT_1=1539,22
PRIMER_LEFT_1_TM=59.269
PRIMER_RIGHT_1_TM=58.655
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=886
PRIMER_PAIR_2_PENALTY=4.113338
PRIMER_LEFT_2_PENALTY=0.691563
PRIMER_RIGHT_2_PENALTY=3.421775
PRIMER_LEFT_2_SEQUENCE=TCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_2_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_2=604,20
PRIMER_RIGHT_2=1539,23
PRIMER_LEFT_2_TM=59.308
PRIMER_RIGHT_2_TM=59.578
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=936
PRIMER_PAIR_3_PENALTY=4.153240
PRIMER_LEFT_3_PENALTY=0.731465
PRIMER_RIGHT_3_PENALTY=3.421775
PRIMER_LEFT_3_SEQUENCE=TTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_3_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_3=654,20
PRIMER_RIGHT_3=1539,23
PRIMER_LEFT_3_TM=59.269
PRIMER_RIGHT_3_TM=59.578
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=886
PRIMER_PAIR_4_PENALTY=4.491216
PRIMER_LEFT_4_PENALTY=1.146144
PRIMER_RIGHT_4_PENALTY=3.345071
PRIMER_LEFT_4_SEQUENCE=GTCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_4_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_4=603,21
PRIMER_RIGHT_4=1539,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=58.655
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=937
PRIMER_PAIR_5_PENALTY=4.567919
PRIMER_LEFT_5_PENALTY=1.146144
PRIMER_RIGHT_5_PENALTY=3.421775
PRIMER_LEFT_5_SEQUENCE=GTCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_5_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_5=603,21
PRIMER_RIGHT_5=1539,23
PRIMER_LEFT_5_TM=60.146
PRIMER_RIGHT_5_TM=59.578
PRIMER_LEFT_5_GC_PERCENT=52.381
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=937
PRIMER_PAIR_6_PENALTY=4.777271
PRIMER_LEFT_6_PENALTY=1.432200
PRIMER_RIGHT_6_PENALTY=3.345071
PRIMER_LEFT_6_SEQUENCE=ATTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_6_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_6=653,21
PRIMER_RIGHT_6=1539,22
PRIMER_LEFT_6_TM=59.568
PRIMER_RIGHT_6_TM=58.655
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=887
PRIMER_PAIR_7_PENALTY=4.804162
PRIMER_LEFT_7_PENALTY=1.459091
PRIMER_RIGHT_7_PENALTY=3.345071
PRIMER_LEFT_7_SEQUENCE=AATCTGGACATCGACCTGAAA
PRIMER_RIGHT_7_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_7=701,21
PRIMER_RIGHT_7=1539,22
PRIMER_LEFT_7_TM=59.541
PRIMER_RIGHT_7_TM=58.655
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=839
PRIMER_PAIR_8_PENALTY=4.853974
PRIMER_LEFT_8_PENALTY=1.432200
PRIMER_RIGHT_8_PENALTY=3.421775
PRIMER_LEFT_8_SEQUENCE=ATTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_8_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_8=653,21
PRIMER_RIGHT_8=1539,23
PRIMER_LEFT_8_TM=59.568
PRIMER_RIGHT_8_TM=59.578
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=887
PRIMER_PAIR_9_PENALTY=4.880866
PRIMER_LEFT_9_PENALTY=1.459091
PRIMER_RIGHT_9_PENALTY=3.421775
PRIMER_LEFT_9_SEQUENCE=AATCTGGACATCGACCTGAAA
PRIMER_RIGHT_9_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_9=701,21
PRIMER_RIGHT_9=1539,23
PRIMER_LEFT_9_TM=59.541
PRIMER_RIGHT_9_TM=59.578
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=839
PRIMER_PAIR_10_PENALTY=5.254549
PRIMER_LEFT_10_PENALTY=1.909478
PRIMER_RIGHT_10_PENALTY=3.345071
PRIMER_LEFT_10_SEQUENCE=TCTTCGGCTTCTCTCGTATGT
PRIMER_RIGHT_10_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_10=607,21
PRIMER_RIGHT_10=1539,22
PRIMER_LEFT_10_TM=59.091
PRIMER_RIGHT_10_TM=58.655
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=45.455
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.6000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=933
PRIMER_PAIR_11_PENALTY=5.274248
PRIMER_LEFT_11_PENALTY=1.929176
PRIMER_RIGHT_11_PENALTY=3.345071
PRIMER_LEFT_11_SEQUENCE=ATCTGGACATCGACCTGAAA
PRIMER_RIGHT_11_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_11=702,20
PRIMER_RIGHT_11=1539,22
PRIMER_LEFT_11_TM=58.071
PRIMER_RIGHT_11_TM=58.655
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=838
PRIMER_PAIR_12_PENALTY=5.331253
PRIMER_LEFT_12_PENALTY=1.909478
PRIMER_RIGHT_12_PENALTY=3.421775
PRIMER_LEFT_12_SEQUENCE=TCTTCGGCTTCTCTCGTATGT
PRIMER_RIGHT_12_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_12=607,21
PRIMER_RIGHT_12=1539,23
PRIMER_LEFT_12_TM=59.091
PRIMER_RIGHT_12_TM=59.578
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=5.6000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=933
PRIMER_PAIR_13_PENALTY=5.337971
PRIMER_LEFT_13_PENALTY=1.992900
PRIMER_RIGHT_13_PENALTY=3.345071
PRIMER_LEFT_13_SEQUENCE=CGGCTTCTCTCGTATGTAAGG
PRIMER_RIGHT_13_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_13=611,21
PRIMER_RIGHT_13=1539,22
PRIMER_LEFT_13_TM=59.007
PRIMER_RIGHT_13_TM=58.655
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.5000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=929
PRIMER_PAIR_14_PENALTY=5.338455
PRIMER_LEFT_14_PENALTY=0.691563
PRIMER_RIGHT_14_PENALTY=4.646892
PRIMER_LEFT_14_SEQUENCE=TCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_14_SEQUENCE=TGACCAATTTACTATGCACCTACC
PRIMER_LEFT_14=604,20
PRIMER_RIGHT_14=1537,24
PRIMER_LEFT_14_TM=59.308
PRIMER_RIGHT_14_TM=59.353
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=41.667
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=934
PRIMER_PAIR_15_PENALTY=5.350951
PRIMER_LEFT_15_PENALTY=1.929176
PRIMER_RIGHT_15_PENALTY=3.421775
PRIMER_LEFT_15_SEQUENCE=ATCTGGACATCGACCTGAAA
PRIMER_RIGHT_15_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_15=702,20
PRIMER_RIGHT_15=1539,23
PRIMER_LEFT_15_TM=58.071
PRIMER_RIGHT_15_TM=59.578
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=838
PRIMER_PAIR_16_PENALTY=5.378357
PRIMER_LEFT_16_PENALTY=0.731465
PRIMER_RIGHT_16_PENALTY=4.646892
PRIMER_LEFT_16_SEQUENCE=TTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_16_SEQUENCE=TGACCAATTTACTATGCACCTACC
PRIMER_LEFT_16=654,20
PRIMER_RIGHT_16=1537,24
PRIMER_LEFT_16_TM=59.269
PRIMER_RIGHT_16_TM=59.353
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=884
PRIMER_PAIR_17_PENALTY=5.414675
PRIMER_LEFT_17_PENALTY=1.992900
PRIMER_RIGHT_17_PENALTY=3.421775
PRIMER_LEFT_17_SEQUENCE=CGGCTTCTCTCGTATGTAAGG
PRIMER_RIGHT_17_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_17=611,21
PRIMER_RIGHT_17=1539,23
PRIMER_LEFT_17_TM=59.007
PRIMER_RIGHT_17_TM=59.578
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.5000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=929
PRIMER_PAIR_18_PENALTY=5.440517
PRIMER_LEFT_18_PENALTY=2.095445
PRIMER_RIGHT_18_PENALTY=3.345071
PRIMER_LEFT_18_SEQUENCE=TTCATTTCTCCTCGTATGGCTT
PRIMER_RIGHT_18_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_18=650,22
PRIMER_RIGHT_18=1539,22
PRIMER_LEFT_18_TM=60.095
PRIMER_RIGHT_18_TM=58.655
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=890
PRIMER_PAIR_19_PENALTY=5.440517
PRIMER_LEFT_19_PENALTY=2.095445
PRIMER_RIGHT_19_PENALTY=3.345071
PRIMER_LEFT_19_SEQUENCE=TTTCATTTCTCCTCGTATGGCT
PRIMER_RIGHT_19_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_19=649,22
PRIMER_RIGHT_19=1539,22
PRIMER_LEFT_19_TM=60.095
PRIMER_RIGHT_19_TM=58.655
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=891
PRIMER_PAIR_20_PENALTY=5.517220
PRIMER_LEFT_20_PENALTY=2.095445
PRIMER_RIGHT_20_PENALTY=3.421775
PRIMER_LEFT_20_SEQUENCE=TTCATTTCTCCTCGTATGGCTT
PRIMER_RIGHT_20_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_20=650,22
PRIMER_RIGHT_20=1539,23
PRIMER_LEFT_20_TM=60.095
PRIMER_RIGHT_20_TM=59.578
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=43.478
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=890
PRIMER_PAIR_21_PENALTY=5.517220
PRIMER_LEFT_21_PENALTY=2.095445
PRIMER_RIGHT_21_PENALTY=3.421775
PRIMER_LEFT_21_SEQUENCE=TTTCATTTCTCCTCGTATGGCT
PRIMER_RIGHT_21_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_21=649,22
PRIMER_RIGHT_21=1539,23
PRIMER_LEFT_21_TM=60.095
PRIMER_RIGHT_21_TM=59.578
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=43.478
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=891
PRIMER_PAIR_22_PENALTY=5.544443
PRIMER_LEFT_22_PENALTY=2.199371
PRIMER_RIGHT_22_PENALTY=3.345071
PRIMER_LEFT_22_SEQUENCE=CATTTCTCCTCGTATGGCTTC
PRIMER_RIGHT_22_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_22=652,21
PRIMER_RIGHT_22=1539,22
PRIMER_LEFT_22_TM=58.801
PRIMER_RIGHT_22_TM=58.655
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=888
PRIMER_PAIR_23_PENALTY=5.544443
PRIMER_LEFT_23_PENALTY=2.199371
PRIMER_RIGHT_23_PENALTY=3.345071
PRIMER_LEFT_23_SEQUENCE=TCATTTCTCCTCGTATGGCTT
PRIMER_RIGHT_23_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_23=651,21
PRIMER_RIGHT_23=1539,22
PRIMER_LEFT_23_TM=58.801
PRIMER_RIGHT_23_TM=58.655
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=889
PRIMER_PAIR_24_PENALTY=5.544443
PRIMER_LEFT_24_PENALTY=2.199371
PRIMER_RIGHT_24_PENALTY=3.345071
PRIMER_LEFT_24_SEQUENCE=TTCATTTCTCCTCGTATGGCT
PRIMER_RIGHT_24_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_24=650,21
PRIMER_RIGHT_24=1539,22
PRIMER_LEFT_24_TM=58.801
PRIMER_RIGHT_24_TM=58.655
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=890
PRIMER_PAIR_25_PENALTY=5.621146
PRIMER_LEFT_25_PENALTY=2.199371
PRIMER_RIGHT_25_PENALTY=3.421775
PRIMER_LEFT_25_SEQUENCE=CATTTCTCCTCGTATGGCTTC
PRIMER_RIGHT_25_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_25=652,21
PRIMER_RIGHT_25=1539,23
PRIMER_LEFT_25_TM=58.801
PRIMER_RIGHT_25_TM=59.578
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=888
PRIMER_PAIR_26_PENALTY=5.621146
PRIMER_LEFT_26_PENALTY=2.199371
PRIMER_RIGHT_26_PENALTY=3.421775
PRIMER_LEFT_26_SEQUENCE=TCATTTCTCCTCGTATGGCTT
PRIMER_RIGHT_26_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_26=651,21
PRIMER_RIGHT_26=1539,23
PRIMER_LEFT_26_TM=58.801
PRIMER_RIGHT_26_TM=59.578
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=889
PRIMER_PAIR_27_PENALTY=5.621146
PRIMER_LEFT_27_PENALTY=2.199371
PRIMER_RIGHT_27_PENALTY=3.421775
PRIMER_LEFT_27_SEQUENCE=TTCATTTCTCCTCGTATGGCT
PRIMER_RIGHT_27_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_27=650,21
PRIMER_RIGHT_27=1539,23
PRIMER_LEFT_27_TM=58.801
PRIMER_RIGHT_27_TM=59.578
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=890
PRIMER_PAIR_28_PENALTY=5.737695
PRIMER_LEFT_28_PENALTY=2.392624
PRIMER_RIGHT_28_PENALTY=3.345071
PRIMER_LEFT_28_SEQUENCE=TTCTTCGGCTTCTCTCGTATGT
PRIMER_RIGHT_28_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_28=606,22
PRIMER_RIGHT_28=1539,22
PRIMER_LEFT_28_TM=60.393
PRIMER_RIGHT_28_TM=58.655
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=5.6000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=934
PRIMER_PAIR_29_PENALTY=5.793036
PRIMER_LEFT_29_PENALTY=1.146144
PRIMER_RIGHT_29_PENALTY=4.646892
PRIMER_LEFT_29_SEQUENCE=GTCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_29_SEQUENCE=TGACCAATTTACTATGCACCTACC
PRIMER_LEFT_29=603,21
PRIMER_RIGHT_29=1537,24
PRIMER_LEFT_29_TM=60.146
PRIMER_RIGHT_29_TM=59.353
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=41.667
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=935
PRIMER_PAIR_30_PENALTY=5.814398
PRIMER_LEFT_30_PENALTY=2.392624
PRIMER_RIGHT_30_PENALTY=3.421775
PRIMER_LEFT_30_SEQUENCE=TTCTTCGGCTTCTCTCGTATGT
PRIMER_RIGHT_30_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_30=606,22
PRIMER_RIGHT_30=1539,23
PRIMER_LEFT_30_TM=60.393
PRIMER_RIGHT_30_TM=59.578
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=43.478
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=5.6000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=934
PRIMER_PAIR_31_PENALTY=5.879606
PRIMER_LEFT_31_PENALTY=0.691563
PRIMER_RIGHT_31_PENALTY=5.188042
PRIMER_LEFT_31_SEQUENCE=TCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_31_SEQUENCE=CTGACCAATTTACTATGCACCTACC
PRIMER_LEFT_31=604,20
PRIMER_RIGHT_31=1538,25
PRIMER_LEFT_31_TM=59.308
PRIMER_RIGHT_31_TM=60.188
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=44.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=935
PRIMER_PAIR_32_PENALTY=5.919507
PRIMER_LEFT_32_PENALTY=0.731465
PRIMER_RIGHT_32_PENALTY=5.188042
PRIMER_LEFT_32_SEQUENCE=TTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_32_SEQUENCE=CTGACCAATTTACTATGCACCTACC
PRIMER_LEFT_32=654,20
PRIMER_RIGHT_32=1538,25
PRIMER_LEFT_32_TM=59.269
PRIMER_RIGHT_32_TM=60.188
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=44.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=885
PRIMER_PAIR_33_PENALTY=5.926879
PRIMER_LEFT_33_PENALTY=2.581808
PRIMER_RIGHT_33_PENALTY=3.345071
PRIMER_LEFT_33_SEQUENCE=TCATTTCTCCTCGTATGGCTTC
PRIMER_RIGHT_33_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_33=651,22
PRIMER_RIGHT_33=1539,22
PRIMER_LEFT_33_TM=60.582
PRIMER_RIGHT_33_TM=58.655
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=889
PRIMER_PAIR_34_PENALTY=5.984860
PRIMER_LEFT_34_PENALTY=2.639789
PRIMER_RIGHT_34_PENALTY=3.345071
PRIMER_LEFT_34_SEQUENCE=TCATTTCTCCTCGTATGGCT
PRIMER_RIGHT_34_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_34=651,20
PRIMER_RIGHT_34=1539,22
PRIMER_LEFT_34_TM=57.360
PRIMER_RIGHT_34_TM=58.655
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=889
PRIMER_PAIR_35_PENALTY=6.003476
PRIMER_LEFT_35_PENALTY=0.691563
PRIMER_RIGHT_35_PENALTY=5.311913
PRIMER_LEFT_35_SEQUENCE=TCTTCTTCGGCTTCTCTCGT
PRIMER_RIGHT_35_SEQUENCE=GCTGACCAATTTACTATGCACCTAC
PRIMER_LEFT_35=604,20
PRIMER_RIGHT_35=1539,25
PRIMER_LEFT_35_TM=59.308
PRIMER_RIGHT_35_TM=60.312
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=44.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=936
PRIMER_PAIR_36_PENALTY=6.003583
PRIMER_LEFT_36_PENALTY=2.581808
PRIMER_RIGHT_36_PENALTY=3.421775
PRIMER_LEFT_36_SEQUENCE=TCATTTCTCCTCGTATGGCTTC
PRIMER_RIGHT_36_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_36=651,22
PRIMER_RIGHT_36=1539,23
PRIMER_LEFT_36_TM=60.582
PRIMER_RIGHT_36_TM=59.578
PRIMER_LEFT_36_GC_PERCENT=45.455
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=889
PRIMER_PAIR_37_PENALTY=6.022320
PRIMER_LEFT_37_PENALTY=2.677248
PRIMER_RIGHT_37_PENALTY=3.345071
PRIMER_LEFT_37_SEQUENCE=TGTCTTCTTCGGCTTCTCTC
PRIMER_RIGHT_37_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_37=602,20
PRIMER_RIGHT_37=1539,22
PRIMER_LEFT_37_TM=57.323
PRIMER_RIGHT_37_TM=58.655
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=938
PRIMER_PAIR_38_PENALTY=6.043378
PRIMER_LEFT_38_PENALTY=0.731465
PRIMER_RIGHT_38_PENALTY=5.311913
PRIMER_LEFT_38_SEQUENCE=TTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_38_SEQUENCE=GCTGACCAATTTACTATGCACCTAC
PRIMER_LEFT_38=654,20
PRIMER_RIGHT_38=1539,25
PRIMER_LEFT_38_TM=59.269
PRIMER_RIGHT_38_TM=60.312
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=44.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=886
PRIMER_PAIR_39_PENALTY=6.061564
PRIMER_LEFT_39_PENALTY=2.639789
PRIMER_RIGHT_39_PENALTY=3.421775
PRIMER_LEFT_39_SEQUENCE=TCATTTCTCCTCGTATGGCT
PRIMER_RIGHT_39_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_39=651,20
PRIMER_RIGHT_39=1539,23
PRIMER_LEFT_39_TM=57.360
PRIMER_RIGHT_39_TM=59.578
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=43.478
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=889
PRIMER_PAIR_40_PENALTY=6.079092
PRIMER_LEFT_40_PENALTY=1.432200
PRIMER_RIGHT_40_PENALTY=4.646892
PRIMER_LEFT_40_SEQUENCE=ATTTCTCCTCGTATGGCTTCC
PRIMER_RIGHT_40_SEQUENCE=TGACCAATTTACTATGCACCTACC
PRIMER_LEFT_40=653,21
PRIMER_RIGHT_40=1537,24
PRIMER_LEFT_40_TM=59.568
PRIMER_RIGHT_40_TM=59.353
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=41.667
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=885
PRIMER_PAIR_41_PENALTY=6.095438
PRIMER_LEFT_41_PENALTY=2.750366
PRIMER_RIGHT_41_PENALTY=3.345071
PRIMER_LEFT_41_SEQUENCE=TCGGCTTCTCTCGTATGTAAGG
PRIMER_RIGHT_41_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_41=610,22
PRIMER_RIGHT_41=1539,22
PRIMER_LEFT_41_TM=60.750
PRIMER_RIGHT_41_TM=58.655
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.5000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=930
PRIMER_PAIR_42_PENALTY=6.099023
PRIMER_LEFT_42_PENALTY=2.677248
PRIMER_RIGHT_42_PENALTY=3.421775
PRIMER_LEFT_42_SEQUENCE=TGTCTTCTTCGGCTTCTCTC
PRIMER_RIGHT_42_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_42=602,20
PRIMER_RIGHT_42=1539,23
PRIMER_LEFT_42_TM=57.323
PRIMER_RIGHT_42_TM=59.578
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=938
PRIMER_PAIR_43_PENALTY=6.105983
PRIMER_LEFT_43_PENALTY=1.459091
PRIMER_RIGHT_43_PENALTY=4.646892
PRIMER_LEFT_43_SEQUENCE=AATCTGGACATCGACCTGAAA
PRIMER_RIGHT_43_SEQUENCE=TGACCAATTTACTATGCACCTACC
PRIMER_LEFT_43=701,21
PRIMER_RIGHT_43=1537,24
PRIMER_LEFT_43_TM=59.541
PRIMER_RIGHT_43_TM=59.353
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=41.667
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=837
PRIMER_PAIR_44_PENALTY=6.171810
PRIMER_LEFT_44_PENALTY=2.826738
PRIMER_RIGHT_44_PENALTY=3.345071
PRIMER_LEFT_44_SEQUENCE=CTTCGGCTTCTCTCGTATGT
PRIMER_RIGHT_44_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_44=608,20
PRIMER_RIGHT_44=1539,22
PRIMER_LEFT_44_TM=57.173
PRIMER_RIGHT_44_TM=58.655
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.455
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=5.6000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=932
PRIMER_PAIR_45_PENALTY=6.172141
PRIMER_LEFT_45_PENALTY=2.750366
PRIMER_RIGHT_45_PENALTY=3.421775
PRIMER_LEFT_45_SEQUENCE=TCGGCTTCTCTCGTATGTAAGG
PRIMER_RIGHT_45_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_45=610,22
PRIMER_RIGHT_45=1539,23
PRIMER_LEFT_45_TM=60.750
PRIMER_RIGHT_45_TM=59.578
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=43.478
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.5000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=930
PRIMER_PAIR_46_PENALTY=6.200693
PRIMER_LEFT_46_PENALTY=2.855622
PRIMER_RIGHT_46_PENALTY=3.345071
PRIMER_LEFT_46_SEQUENCE=AAATCTGGACATCGACCTGAAA
PRIMER_RIGHT_46_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_46=700,22
PRIMER_RIGHT_46=1539,22
PRIMER_LEFT_46_TM=60.856
PRIMER_RIGHT_46_TM=58.655
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=840
PRIMER_PAIR_47_PENALTY=6.248513
PRIMER_LEFT_47_PENALTY=2.826738
PRIMER_RIGHT_47_PENALTY=3.421775
PRIMER_LEFT_47_SEQUENCE=CTTCGGCTTCTCTCGTATGT
PRIMER_RIGHT_47_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_47=608,20
PRIMER_RIGHT_47=1539,23
PRIMER_LEFT_47_TM=57.173
PRIMER_RIGHT_47_TM=59.578
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=5.6000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=932
PRIMER_PAIR_48_PENALTY=6.257637
PRIMER_LEFT_48_PENALTY=2.912566
PRIMER_RIGHT_48_PENALTY=3.345071
PRIMER_LEFT_48_SEQUENCE=TGTTTCATTTCTCCTCGTATGG
PRIMER_RIGHT_48_SEQUENCE=GCTGACCAATTTACTATGCACC
PRIMER_LEFT_48=647,22
PRIMER_RIGHT_48=1539,22
PRIMER_LEFT_48_TM=59.087
PRIMER_RIGHT_48_TM=58.655
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.4000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=893
PRIMER_PAIR_49_PENALTY=6.277396
PRIMER_LEFT_49_PENALTY=2.855622
PRIMER_RIGHT_49_PENALTY=3.421775
PRIMER_LEFT_49_SEQUENCE=AAATCTGGACATCGACCTGAAA
PRIMER_RIGHT_49_SEQUENCE=GCTGACCAATTTACTATGCACCT
PRIMER_LEFT_49=700,22
PRIMER_RIGHT_49=1539,23
PRIMER_LEFT_49_TM=60.856
PRIMER_RIGHT_49_TM=59.578
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=43.478
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=840
=
SEQUENCE_ID=MAGIC.446_3_4633961_4634153_bur-0_ct-1_227237_353266_TRUE
SEQUENCE_TARGET=950,292
SEQUENCE_TEMPLATE=CCCAGAACACGGTTTGCAGCTTTTGTAAGCACCTGATGCTTTTACTGCCATGTCTTTAATCTGAAGATTCACATACAAGAATCACATGNTATTCACAAAATTAAATACACAGTGAAANNNNNNNNNAAAGTCACGGACTTAAATTGATTTGTTCTTAGCTTCCAAACAGAAACCAACAAGAGCAAAAAATGGTCCTTTTCTCATTTGTGTTTTTTTTTCGTGAAAGTGNNACAGAGGAAGAATGTTTATCTCTACCTGTGACGTCAGGGACTTAATCTGCTTAGACCTGGGTGTTTCAATAACTCTGTCTTCTTCTTCTTCATCCTCTTCTNGTTTCTTAGATCCACCATTGTTGGTGTTTAGCTGCTTCGTACAAGCTATGCATGTCAGCATCTCTATCACTCACTAAAGAGTTTCAATTTCGACCTGAGAATTTAGAAGAATGAAACCCAAGTCACTTCCTCCACCAAAAATAATCAAATCTAAGAAATGAGAACCCAAAAAGGCTCATAATTTGTACCTTTTTTTGCAACAGTAGTTACGCACTTCAACTTCTACCNTCCAATATATAAACNCAACACTCACTAAAGTCGATTGCTTTTCTCTCAACCACAGAGATTCAGATGTAAAACNCCACCNGTGAAATGACGGCNAAGGGAAGGAGGAGACCGTTGCGGCAGGGGAAAATTAGGGTACCAGGATCTGTAGATCAGACCTAGAAACGACTTCGCATTAAAGACTCTTCTTTTTTTTNAAACTCTCTGGGTCGGTTTACTTGTGGCTTNAAAGCTCTTATCAGAGAGATTGAGTGAGATACACTCTTAAATCTCAACACTTTATGAAATCCAAAGCCGAATGCAGAAGCAAAATGCAAAAAAGCTACGAATTTTTGGGGAGAGATANAGAGAGAGANGAGCTTTGATATTTGGGCTTTGAGATCGTCGGACACTNTGAAAGTTTGCGCACAAGTGTGGTGTTGTGTTCCTCCTCCTTCCTAANTNCTATANNNNNNAAAAAAGCTCNACTCTTTTCTTTTTCTTCAGAGAAAAAAAAACAGTAATGGCTATTTTCAGATTCGTCTTTAAGTTTGATANTTATGGATTATTATGACCNAAACCAAAAANAAACAAAAACGTAACACAGTTTTTACTAACATAGATTAACCAATTNAATAGTTCTACAGNTTTATTGCNANNTTTTAATATCCGCATTTTGTCAACTTTTGATTTATTATTTATANTAAATTACTTAGTTTTGAATCGTCTTGTATTTCATTTGCNAACANAACTAGTAGTATNTTCTTNAATTTTACTGCTTTGAGAGTGAAGAAATCGGACCAAGAAAATTTCAAATGTTATTCTCTTCTTTATTTCCTAGNTTTTTTTCTTTATTCTCTTTTCCCTTTTCTTGCNTTTTTNNNNANAAAAGAACAAATAAAACTATTTTCATAGAAATAATGAAGCCGTGCAAAATATCCATGGTGNGGCCANATATTGACACGTGCATTTTTAAATGATTTTGGCTGTCTAATTTTGCACCNAAATAAAGGAAAAGAAAAAGGAAACCAAATAACTAATCCATTTTGTTATCTGTCAATTTTCTNAATAACGAGTATTTCATAATTTAAGACAGTGACTCTGGGAAATGTGACAGAATGTTACTAGTTGGTAATTACTCCTAATTTATTCAAACATTTAGAGGCAACTAGTCGTTTAAATTATTTTAAATCAACTGCATTCAAATATGAATGCANAAACCNAAAAAGGAATATTTACGAGTCTATATAATGTCAGATTTGGTCACAGGAGCGCCGTGAAGCAACGGGCGAGAATTAACTACACTCTGTGGAGTTTCTTACCATACACTATTTTACAATTCGTAGTTAATGTTTTTANTTTTTTTNTTGGCTTGCTTTCTCTATTTTTTTCACTAATACAGCTGAAACATTTTTTTNAATTAGGACAAAAGAAAACAGTTTTTATAGCAATGAATGATCAGATCCGATACTGCAAAATATAAATTAATTTAATTTTTACCATACCACGTGAATGATCTGTGTTTTCTTGTTTGATATTGTTCTTTCTCTTTCCATTTTAATNTTAGCTNGTATCTTATAGCTATAACTATGACCTATCCGTTACAAATAATACTTTATGATGATCTCTCTTTCACCCCATGTTAAATTGAACATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.661358
PRIMER_LEFT_0_PENALTY=0.099438
PRIMER_RIGHT_0_PENALTY=0.561921
PRIMER_LEFT_0_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_0_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_0=838,20
PRIMER_RIGHT_0=1478,20
PRIMER_LEFT_0_TM=59.901
PRIMER_RIGHT_0_TM=59.438
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=641
PRIMER_PAIR_1_PENALTY=0.956013
PRIMER_LEFT_1_PENALTY=0.099438
PRIMER_RIGHT_1_PENALTY=0.856575
PRIMER_LEFT_1_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_1_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_1=838,20
PRIMER_RIGHT_1=1479,20
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.857
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=642
PRIMER_PAIR_2_PENALTY=0.963140
PRIMER_LEFT_2_PENALTY=0.401220
PRIMER_RIGHT_2_PENALTY=0.561921
PRIMER_LEFT_2_SEQUENCE=CTGGGTCGGTTTACTTGTGG
PRIMER_RIGHT_2_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_2=761,20
PRIMER_RIGHT_2=1478,20
PRIMER_LEFT_2_TM=60.401
PRIMER_RIGHT_2_TM=59.438
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=718
PRIMER_PAIR_3_PENALTY=1.070572
PRIMER_LEFT_3_PENALTY=0.099438
PRIMER_RIGHT_3_PENALTY=0.971135
PRIMER_LEFT_3_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_3_SEQUENCE=GGATATTTTGCACGGCTTCA
PRIMER_LEFT_3=838,20
PRIMER_RIGHT_3=1476,20
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=60.971
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=639
PRIMER_PAIR_4_PENALTY=1.070572
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.971135
PRIMER_LEFT_4_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_4_SEQUENCE=TGGATATTTTGCACGGCTTC
PRIMER_LEFT_4=838,20
PRIMER_RIGHT_4=1477,20
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=60.971
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=640
PRIMER_PAIR_5_PENALTY=1.223362
PRIMER_LEFT_5_PENALTY=0.099438
PRIMER_RIGHT_5_PENALTY=1.123924
PRIMER_LEFT_5_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_5_SEQUENCE=GGTGCAAAATTAGACAGCCAA
PRIMER_LEFT_5=838,20
PRIMER_RIGHT_5=1538,21
PRIMER_LEFT_5_TM=59.901
PRIMER_RIGHT_5_TM=60.124
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=10.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=701
PRIMER_PAIR_6_PENALTY=1.257795
PRIMER_LEFT_6_PENALTY=0.401220
PRIMER_RIGHT_6_PENALTY=0.856575
PRIMER_LEFT_6_SEQUENCE=CTGGGTCGGTTTACTTGTGG
PRIMER_RIGHT_6_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_6=761,20
PRIMER_RIGHT_6=1479,20
PRIMER_LEFT_6_TM=60.401
PRIMER_RIGHT_6_TM=60.857
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=11.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=719
PRIMER_PAIR_7_PENALTY=1.306845
PRIMER_LEFT_7_PENALTY=0.744925
PRIMER_RIGHT_7_PENALTY=0.561921
PRIMER_LEFT_7_SEQUENCE=TGAGAACCCAAAAAGGCTCA
PRIMER_RIGHT_7_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_7=490,20
PRIMER_RIGHT_7=1478,20
PRIMER_LEFT_7_TM=60.745
PRIMER_RIGHT_7_TM=59.438
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=989
PRIMER_PAIR_8_PENALTY=1.326339
PRIMER_LEFT_8_PENALTY=0.099438
PRIMER_RIGHT_8_PENALTY=1.226901
PRIMER_LEFT_8_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_8_SEQUENCE=GGTGCAAAATTAGACAGCCA
PRIMER_LEFT_8=838,20
PRIMER_RIGHT_8=1538,20
PRIMER_LEFT_8_TM=59.901
PRIMER_RIGHT_8_TM=58.773
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=701
PRIMER_PAIR_9_PENALTY=1.328221
PRIMER_LEFT_9_PENALTY=0.099438
PRIMER_RIGHT_9_PENALTY=1.228784
PRIMER_LEFT_9_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_9_SEQUENCE=TTGCACGGCTTCATTATTTC
PRIMER_LEFT_9=838,20
PRIMER_RIGHT_9=1469,20
PRIMER_LEFT_9_TM=59.901
PRIMER_RIGHT_9_TM=58.771
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=632
PRIMER_PAIR_10_PENALTY=1.372355
PRIMER_LEFT_10_PENALTY=0.401220
PRIMER_RIGHT_10_PENALTY=0.971135
PRIMER_LEFT_10_SEQUENCE=CTGGGTCGGTTTACTTGTGG
PRIMER_RIGHT_10_SEQUENCE=GGATATTTTGCACGGCTTCA
PRIMER_LEFT_10=761,20
PRIMER_RIGHT_10=1476,20
PRIMER_LEFT_10_TM=60.401
PRIMER_RIGHT_10_TM=60.971
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=716
PRIMER_PAIR_11_PENALTY=1.372355
PRIMER_LEFT_11_PENALTY=0.401220
PRIMER_RIGHT_11_PENALTY=0.971135
PRIMER_LEFT_11_SEQUENCE=CTGGGTCGGTTTACTTGTGG
PRIMER_RIGHT_11_SEQUENCE=TGGATATTTTGCACGGCTTC
PRIMER_LEFT_11=761,20
PRIMER_RIGHT_11=1477,20
PRIMER_LEFT_11_TM=60.401
PRIMER_RIGHT_11_TM=60.971
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=717
PRIMER_PAIR_12_PENALTY=1.373803
PRIMER_LEFT_12_PENALTY=0.811883
PRIMER_RIGHT_12_PENALTY=0.561921
PRIMER_LEFT_12_SEQUENCE=GCTTTGAGATCGTCGGACAC
PRIMER_RIGHT_12_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_12=929,20
PRIMER_RIGHT_12=1478,20
PRIMER_LEFT_12_TM=60.812
PRIMER_RIGHT_12_TM=59.438
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.1000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=550
PRIMER_PAIR_13_PENALTY=1.381028
PRIMER_LEFT_13_PENALTY=0.099438
PRIMER_RIGHT_13_PENALTY=1.281590
PRIMER_LEFT_13_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_13_SEQUENCE=GCTCCTGTGACCAAATCTGAC
PRIMER_LEFT_13=838,20
PRIMER_RIGHT_13=1808,21
PRIMER_LEFT_13_TM=59.901
PRIMER_RIGHT_13_TM=59.718
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=971
PRIMER_PAIR_14_PENALTY=1.403462
PRIMER_LEFT_14_PENALTY=0.841542
PRIMER_RIGHT_14_PENALTY=0.561921
PRIMER_LEFT_14_SEQUENCE=GCTACGAATTTTTGGGGAGA
PRIMER_RIGHT_14_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_14=878,20
PRIMER_RIGHT_14=1478,20
PRIMER_LEFT_14_TM=59.158
PRIMER_RIGHT_14_TM=59.438
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=601
PRIMER_PAIR_15_PENALTY=1.460982
PRIMER_LEFT_15_PENALTY=0.899062
PRIMER_RIGHT_15_PENALTY=0.561921
PRIMER_LEFT_15_SEQUENCE=GGTCGGTTTACTTGTGGCTT
PRIMER_RIGHT_15_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_15=764,20
PRIMER_RIGHT_15=1478,20
PRIMER_LEFT_15_TM=59.101
PRIMER_RIGHT_15_TM=59.438
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=715
PRIMER_PAIR_16_PENALTY=1.584284
PRIMER_LEFT_16_PENALTY=0.099438
PRIMER_RIGHT_16_PENALTY=1.484846
PRIMER_LEFT_16_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_16_SEQUENCE=GTTAATTCTCGCCCGTTGCT
PRIMER_LEFT_16=838,20
PRIMER_RIGHT_16=1835,20
PRIMER_LEFT_16_TM=59.901
PRIMER_RIGHT_16_TM=61.485
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=998
PRIMER_PAIR_17_PENALTY=1.601500
PRIMER_LEFT_17_PENALTY=0.744925
PRIMER_RIGHT_17_PENALTY=0.856575
PRIMER_LEFT_17_SEQUENCE=TGAGAACCCAAAAAGGCTCA
PRIMER_RIGHT_17_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_17=490,20
PRIMER_RIGHT_17=1479,20
PRIMER_LEFT_17_TM=60.745
PRIMER_RIGHT_17_TM=60.857
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=11.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=990
PRIMER_PAIR_18_PENALTY=1.628121
PRIMER_LEFT_18_PENALTY=0.401220
PRIMER_RIGHT_18_PENALTY=1.226901
PRIMER_LEFT_18_SEQUENCE=CTGGGTCGGTTTACTTGTGG
PRIMER_RIGHT_18_SEQUENCE=GGTGCAAAATTAGACAGCCA
PRIMER_LEFT_18=761,20
PRIMER_RIGHT_18=1538,20
PRIMER_LEFT_18_TM=60.401
PRIMER_RIGHT_18_TM=58.773
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=778
PRIMER_PAIR_19_PENALTY=1.630003
PRIMER_LEFT_19_PENALTY=0.401220
PRIMER_RIGHT_19_PENALTY=1.228784
PRIMER_LEFT_19_SEQUENCE=CTGGGTCGGTTTACTTGTGG
PRIMER_RIGHT_19_SEQUENCE=TTGCACGGCTTCATTATTTC
PRIMER_LEFT_19=761,20
PRIMER_RIGHT_19=1469,20
PRIMER_LEFT_19_TM=60.401
PRIMER_RIGHT_19_TM=58.771
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=709
PRIMER_PAIR_20_PENALTY=1.643785
PRIMER_LEFT_20_PENALTY=1.081865
PRIMER_RIGHT_20_PENALTY=0.561921
PRIMER_LEFT_20_SEQUENCE=GCTACGAATTTTTGGGGAGAG
PRIMER_RIGHT_20_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_20=878,21
PRIMER_RIGHT_20=1478,20
PRIMER_LEFT_20_TM=60.082
PRIMER_RIGHT_20_TM=59.438
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=601
PRIMER_PAIR_21_PENALTY=1.643785
PRIMER_LEFT_21_PENALTY=1.081865
PRIMER_RIGHT_21_PENALTY=0.561921
PRIMER_LEFT_21_SEQUENCE=AGCTACGAATTTTTGGGGAGA
PRIMER_RIGHT_21_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_21=877,21
PRIMER_RIGHT_21=1478,20
PRIMER_LEFT_21_TM=60.082
PRIMER_RIGHT_21_TM=59.438
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=602
PRIMER_PAIR_22_PENALTY=1.668458
PRIMER_LEFT_22_PENALTY=0.811883
PRIMER_RIGHT_22_PENALTY=0.856575
PRIMER_LEFT_22_SEQUENCE=GCTTTGAGATCGTCGGACAC
PRIMER_RIGHT_22_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_22=929,20
PRIMER_RIGHT_22=1479,20
PRIMER_LEFT_22_TM=60.812
PRIMER_RIGHT_22_TM=60.857
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.1000
PRIMER_RIGHT_22_END_STABILITY=11.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=551
PRIMER_PAIR_23_PENALTY=1.698117
PRIMER_LEFT_23_PENALTY=0.841542
PRIMER_RIGHT_23_PENALTY=0.856575
PRIMER_LEFT_23_SEQUENCE=GCTACGAATTTTTGGGGAGA
PRIMER_RIGHT_23_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_23=878,20
PRIMER_RIGHT_23=1479,20
PRIMER_LEFT_23_TM=59.158
PRIMER_RIGHT_23_TM=60.857
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=11.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=602
PRIMER_PAIR_24_PENALTY=1.711673
PRIMER_LEFT_24_PENALTY=0.099438
PRIMER_RIGHT_24_PENALTY=1.612236
PRIMER_LEFT_24_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_24_SEQUENCE=TGCACGGCTTCATTATTTCT
PRIMER_LEFT_24=838,20
PRIMER_RIGHT_24=1468,20
PRIMER_LEFT_24_TM=59.901
PRIMER_RIGHT_24_TM=58.388
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=631
PRIMER_PAIR_25_PENALTY=1.716060
PRIMER_LEFT_25_PENALTY=0.744925
PRIMER_RIGHT_25_PENALTY=0.971135
PRIMER_LEFT_25_SEQUENCE=TGAGAACCCAAAAAGGCTCA
PRIMER_RIGHT_25_SEQUENCE=GGATATTTTGCACGGCTTCA
PRIMER_LEFT_25=490,20
PRIMER_RIGHT_25=1476,20
PRIMER_LEFT_25_TM=60.745
PRIMER_RIGHT_25_TM=60.971
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=987
PRIMER_PAIR_26_PENALTY=1.716060
PRIMER_LEFT_26_PENALTY=0.744925
PRIMER_RIGHT_26_PENALTY=0.971135
PRIMER_LEFT_26_SEQUENCE=TGAGAACCCAAAAAGGCTCA
PRIMER_RIGHT_26_SEQUENCE=TGGATATTTTGCACGGCTTC
PRIMER_LEFT_26=490,20
PRIMER_RIGHT_26=1477,20
PRIMER_LEFT_26_TM=60.745
PRIMER_RIGHT_26_TM=60.971
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=988
PRIMER_PAIR_27_PENALTY=1.755637
PRIMER_LEFT_27_PENALTY=0.899062
PRIMER_RIGHT_27_PENALTY=0.856575
PRIMER_LEFT_27_SEQUENCE=GGTCGGTTTACTTGTGGCTT
PRIMER_RIGHT_27_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_27=764,20
PRIMER_RIGHT_27=1479,20
PRIMER_LEFT_27_TM=59.101
PRIMER_RIGHT_27_TM=60.857
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=11.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=716
PRIMER_PAIR_28_PENALTY=1.783018
PRIMER_LEFT_28_PENALTY=0.811883
PRIMER_RIGHT_28_PENALTY=0.971135
PRIMER_LEFT_28_SEQUENCE=GCTTTGAGATCGTCGGACAC
PRIMER_RIGHT_28_SEQUENCE=GGATATTTTGCACGGCTTCA
PRIMER_LEFT_28=929,20
PRIMER_RIGHT_28=1476,20
PRIMER_LEFT_28_TM=60.812
PRIMER_RIGHT_28_TM=60.971
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.1000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=548
PRIMER_PAIR_29_PENALTY=1.783018
PRIMER_LEFT_29_PENALTY=0.811883
PRIMER_RIGHT_29_PENALTY=0.971135
PRIMER_LEFT_29_SEQUENCE=GCTTTGAGATCGTCGGACAC
PRIMER_RIGHT_29_SEQUENCE=TGGATATTTTGCACGGCTTC
PRIMER_LEFT_29=929,20
PRIMER_RIGHT_29=1477,20
PRIMER_LEFT_29_TM=60.812
PRIMER_RIGHT_29_TM=60.971
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.1000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=549
PRIMER_PAIR_30_PENALTY=1.812676
PRIMER_LEFT_30_PENALTY=0.841542
PRIMER_RIGHT_30_PENALTY=0.971135
PRIMER_LEFT_30_SEQUENCE=GCTACGAATTTTTGGGGAGA
PRIMER_RIGHT_30_SEQUENCE=GGATATTTTGCACGGCTTCA
PRIMER_LEFT_30=878,20
PRIMER_RIGHT_30=1476,20
PRIMER_LEFT_30_TM=59.158
PRIMER_RIGHT_30_TM=60.971
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=599
PRIMER_PAIR_31_PENALTY=1.812676
PRIMER_LEFT_31_PENALTY=0.841542
PRIMER_RIGHT_31_PENALTY=0.971135
PRIMER_LEFT_31_SEQUENCE=GCTACGAATTTTTGGGGAGA
PRIMER_RIGHT_31_SEQUENCE=TGGATATTTTGCACGGCTTC
PRIMER_LEFT_31=878,20
PRIMER_RIGHT_31=1477,20
PRIMER_LEFT_31_TM=59.158
PRIMER_RIGHT_31_TM=60.971
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=600
PRIMER_PAIR_32_PENALTY=1.825751
PRIMER_LEFT_32_PENALTY=0.099438
PRIMER_RIGHT_32_PENALTY=1.726313
PRIMER_LEFT_32_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_32_SEQUENCE=GGTCCGATTTCTTCACTCTCA
PRIMER_LEFT_32=838,20
PRIMER_RIGHT_32=1337,21
PRIMER_LEFT_32_TM=59.901
PRIMER_RIGHT_32_TM=59.274
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=500
PRIMER_PAIR_33_PENALTY=1.825751
PRIMER_LEFT_33_PENALTY=0.099438
PRIMER_RIGHT_33_PENALTY=1.726313
PRIMER_LEFT_33_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_33_SEQUENCE=TGGTCCGATTTCTTCACTCTC
PRIMER_LEFT_33=838,20
PRIMER_RIGHT_33=1338,21
PRIMER_LEFT_33_TM=59.901
PRIMER_RIGHT_33_TM=59.274
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=501
PRIMER_PAIR_34_PENALTY=1.829209
PRIMER_LEFT_34_PENALTY=0.099438
PRIMER_RIGHT_34_PENALTY=1.729772
PRIMER_LEFT_34_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_34_SEQUENCE=TTCTTGGTCCGATTTCTTCA
PRIMER_LEFT_34=838,20
PRIMER_RIGHT_34=1342,20
PRIMER_LEFT_34_TM=59.901
PRIMER_RIGHT_34_TM=58.270
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=505
PRIMER_PAIR_35_PENALTY=1.855936
PRIMER_LEFT_35_PENALTY=1.294015
PRIMER_RIGHT_35_PENALTY=0.561921
PRIMER_LEFT_35_SEQUENCE=GGGTCGGTTTACTTGTGGCT
PRIMER_RIGHT_35_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_35=763,20
PRIMER_RIGHT_35=1478,20
PRIMER_LEFT_35_TM=61.294
PRIMER_RIGHT_35_TM=59.438
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=716
PRIMER_PAIR_36_PENALTY=1.868138
PRIMER_LEFT_36_PENALTY=1.306217
PRIMER_RIGHT_36_PENALTY=0.561921
PRIMER_LEFT_36_SEQUENCE=AAGCTACGAATTTTTGGGGA
PRIMER_RIGHT_36_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_36=876,20
PRIMER_RIGHT_36=1478,20
PRIMER_LEFT_36_TM=58.694
PRIMER_RIGHT_36_TM=59.438
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=603
PRIMER_PAIR_37_PENALTY=1.870197
PRIMER_LEFT_37_PENALTY=0.899062
PRIMER_RIGHT_37_PENALTY=0.971135
PRIMER_LEFT_37_SEQUENCE=GGTCGGTTTACTTGTGGCTT
PRIMER_RIGHT_37_SEQUENCE=GGATATTTTGCACGGCTTCA
PRIMER_LEFT_37=764,20
PRIMER_RIGHT_37=1476,20
PRIMER_LEFT_37_TM=59.101
PRIMER_RIGHT_37_TM=60.971
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=713
PRIMER_PAIR_38_PENALTY=1.870197
PRIMER_LEFT_38_PENALTY=0.899062
PRIMER_RIGHT_38_PENALTY=0.971135
PRIMER_LEFT_38_SEQUENCE=GGTCGGTTTACTTGTGGCTT
PRIMER_RIGHT_38_SEQUENCE=TGGATATTTTGCACGGCTTC
PRIMER_LEFT_38=764,20
PRIMER_RIGHT_38=1477,20
PRIMER_LEFT_38_TM=59.101
PRIMER_RIGHT_38_TM=60.971
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=714
PRIMER_PAIR_39_PENALTY=1.919942
PRIMER_LEFT_39_PENALTY=1.358022
PRIMER_RIGHT_39_PENALTY=0.561921
PRIMER_LEFT_39_SEQUENCE=CTCTGGGTCGGTTTACTTGTG
PRIMER_RIGHT_39_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_39=759,21
PRIMER_RIGHT_39=1478,20
PRIMER_LEFT_39_TM=59.642
PRIMER_RIGHT_39_TM=59.438
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=720
PRIMER_PAIR_40_PENALTY=1.933489
PRIMER_LEFT_40_PENALTY=1.371568
PRIMER_RIGHT_40_PENALTY=0.561921
PRIMER_LEFT_40_SEQUENCE=CGATTGCTTTTCTCTCAACCA
PRIMER_RIGHT_40_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_40=591,21
PRIMER_RIGHT_40=1478,20
PRIMER_LEFT_40_TM=60.372
PRIMER_RIGHT_40_TM=59.438
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=888
PRIMER_PAIR_41_PENALTY=1.935807
PRIMER_LEFT_41_PENALTY=0.811883
PRIMER_RIGHT_41_PENALTY=1.123924
PRIMER_LEFT_41_SEQUENCE=GCTTTGAGATCGTCGGACAC
PRIMER_RIGHT_41_SEQUENCE=GGTGCAAAATTAGACAGCCAA
PRIMER_LEFT_41=929,20
PRIMER_RIGHT_41=1538,21
PRIMER_LEFT_41_TM=60.812
PRIMER_RIGHT_41_TM=60.124
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.1000
PRIMER_RIGHT_41_END_STABILITY=10.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=610
PRIMER_PAIR_42_PENALTY=1.938440
PRIMER_LEFT_42_PENALTY=1.081865
PRIMER_RIGHT_42_PENALTY=0.856575
PRIMER_LEFT_42_SEQUENCE=GCTACGAATTTTTGGGGAGAG
PRIMER_RIGHT_42_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_42=878,21
PRIMER_RIGHT_42=1479,20
PRIMER_LEFT_42_TM=60.082
PRIMER_RIGHT_42_TM=60.857
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=11.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=602
PRIMER_PAIR_43_PENALTY=1.938440
PRIMER_LEFT_43_PENALTY=1.081865
PRIMER_RIGHT_43_PENALTY=0.856575
PRIMER_LEFT_43_SEQUENCE=AGCTACGAATTTTTGGGGAGA
PRIMER_RIGHT_43_SEQUENCE=CATGGATATTTTGCACGGCT
PRIMER_LEFT_43=877,21
PRIMER_RIGHT_43=1479,20
PRIMER_LEFT_43_TM=60.082
PRIMER_RIGHT_43_TM=60.857
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=11.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=603
PRIMER_PAIR_44_PENALTY=1.939229
PRIMER_LEFT_44_PENALTY=0.099438
PRIMER_RIGHT_44_PENALTY=1.839792
PRIMER_LEFT_44_SEQUENCE=ATGAAATCCAAAGCCGAATG
PRIMER_RIGHT_44_SEQUENCE=TTCTTGGTCCGATTTCTTCAC
PRIMER_LEFT_44=838,20
PRIMER_RIGHT_44=1342,21
PRIMER_LEFT_44_TM=59.901
PRIMER_RIGHT_44_TM=59.160
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=505
PRIMER_PAIR_45_PENALTY=1.940774
PRIMER_LEFT_45_PENALTY=1.378854
PRIMER_RIGHT_45_PENALTY=0.561921
PRIMER_LEFT_45_SEQUENCE=TCTGGGTCGGTTTACTTGTG
PRIMER_RIGHT_45_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_45=760,20
PRIMER_RIGHT_45=1478,20
PRIMER_LEFT_45_TM=58.621
PRIMER_RIGHT_45_TM=59.438
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=719
PRIMER_PAIR_46_PENALTY=1.944794
PRIMER_LEFT_46_PENALTY=1.382873
PRIMER_RIGHT_46_PENALTY=0.561921
PRIMER_LEFT_46_SEQUENCE=AAGCTACGAATTTTTGGGGAG
PRIMER_RIGHT_46_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_46=876,21
PRIMER_RIGHT_46=1478,20
PRIMER_LEFT_46_TM=59.617
PRIMER_RIGHT_46_TM=59.438
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=9.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=603
PRIMER_PAIR_47_PENALTY=1.965466
PRIMER_LEFT_47_PENALTY=0.841542
PRIMER_RIGHT_47_PENALTY=1.123924
PRIMER_LEFT_47_SEQUENCE=GCTACGAATTTTTGGGGAGA
PRIMER_RIGHT_47_SEQUENCE=GGTGCAAAATTAGACAGCCAA
PRIMER_LEFT_47=878,20
PRIMER_RIGHT_47=1538,21
PRIMER_LEFT_47_TM=59.158
PRIMER_RIGHT_47_TM=60.124
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=10.0000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=661
PRIMER_PAIR_48_PENALTY=1.973708
PRIMER_LEFT_48_PENALTY=0.744925
PRIMER_RIGHT_48_PENALTY=1.228784
PRIMER_LEFT_48_SEQUENCE=TGAGAACCCAAAAAGGCTCA
PRIMER_RIGHT_48_SEQUENCE=TTGCACGGCTTCATTATTTC
PRIMER_LEFT_48=490,20
PRIMER_RIGHT_48=1469,20
PRIMER_LEFT_48_TM=60.745
PRIMER_RIGHT_48_TM=58.771
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=980
PRIMER_PAIR_49_PENALTY=1.977177
PRIMER_LEFT_49_PENALTY=1.415257
PRIMER_RIGHT_49_PENALTY=0.561921
PRIMER_LEFT_49_SEQUENCE=AATGAGAACCCAAAAAGGCTC
PRIMER_RIGHT_49_SEQUENCE=ATGGATATTTTGCACGGCTT
PRIMER_LEFT_49=488,21
PRIMER_RIGHT_49=1478,20
PRIMER_LEFT_49_TM=59.585
PRIMER_RIGHT_49_TM=59.438
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=991
=
SEQUENCE_ID=MAGIC.433_2_1076109_1076318_sf-2_kn-0_282125_113780_TRUE
SEQUENCE_TARGET=950,309
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTGGCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGACCTANANNACCGCNTAAAACTAGTAAAACATTGAATGNCAATTGTGTGAAAGAAAACCCATTCCATGCTTTGCTNTCAATGGATATATAACCATGATTCATTTGANNATGTGATTTGCTTTCCTCNATATAATANTGAGGACATCGAATTGTCATNTNNNTGTCACATCCATATGAGTCAGGANACTTATNANAGTCCAACCATTTGCATNCAATTATTGTTAGTAGNANAGACCCCATCATCACTTTCACGCNCTACNTCTNCGCTTANAAAAANACTCTTCTTATCTTCTTCTCNTCGTCTTCACTCATCTCTAACGTTTCTTTCTATCTNNAATCCAAGCAACAANATGGATNCCGNNATNNCTTTCGACAGATCTCCGATGTTTCGAGTCTACAAGAGTGGCCGCATTGAGCGTCTCTTAGGAGAAACAACCGTCCCACCATCTCTTACACCACAAAACGGNGTCGTCTCCAAAGATATCATCCATTCACCGGAGAAAAACCTCTCCCTCCGCATCTACCTACCNGAGAAANTCNCNGNNAAAAANCTNCCAATCCTCATNTACTTCCACGGTGGTGGTTTCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_1952355_1952953_rsch-4_oy-0_99315_972477_TRUE
SEQUENCE_TARGET=950,698
SEQUENCE_TEMPLATE=GTGGGAAAAGGNATATAAACCGTATACCAAAATTTTTGCCANAGGGAGTNTGGNACACTATCAANAGATCCTTCAAAACATGAAGGAAGATGAGCTNNCNANTNAAATCTCATCTCTTTGTCANTATNCATTTGTTNTATATTNANTNCNTAGATTATATTNTTTCAGATTAAGTGGCAGCCTTATAGCAAAGTTGAATTGCNCGAATACACCTCTCAATTGCGTATGGTGTTTGCANAAGNNNCATNCATNNGCTTTAACACCATCNCATANAATTGTCNNGGTACTTTCTNNAANCAATTAGGACNGGAGGAGTCAGACATACGGNTGCCACCNGAAATAAAGCCGAANNNGNCGTCTAAGTTAAGCAGACACAAAGACAAGNACTGGAGAGAGCATAANAGTTTCTACTCACANATGAATTTGGCNTGGGAAAANCGAGATAATCGTTTGGTGTATCAACGACACAATNAGGAATNTGAGGNGAGGTGATTTTAATNTTTTTTTNNAGTCGATTGTGTTTACATGTAGGTAGGAGGAAACTNGTTTTTCTTTTTTAAACACAAGTGCTTCATGAANCACATGTGTTTTAGATAATGTGTTTNTTTTTCTAACAGAAGTGCCTCATGGAATCCACTTGGTCTATACTGANTNTTTTTTAACACAACTGCCTCATGAAACACCTGNNCGTTATATAATGTGNTTGTTTTTCTAACANAAGTGCCTCATGGAACCCGCTNGGTCTATATATTTANGTTTTTTNATGATTTTTTNTTTTGTTTTGATAGATGAATTTNGGCTNCCATTCCCGGTCTATCTCANGAAGAGGTGGAGTTGAGGCCATCACCAATGATNCGGTATTTGTTGTNTTTANATTTTTTTATCTTTTTATCNCNAAATGTTTNTATATTAAACTTATATAAATGGTGAATCTGGATAGGTTGATAATCAAGATGAATCAGATGAAATCCCTTTCCCTATCTCAGTTGAGAACNTCACANGTGCCANCAACAATGATCAGGTATTTGTTGTGTTTAAAGTTTTATCTTTTTATCTCCAAATGTTTATTTAATAAACTTATATAAATGGTGAGTAAGTCAATTGGGATTTANCTNTGGTTTTTCTTGAATTGTGTTTATGATTATTTGTTTTGTTTGATAGATGAATCTCGANGCCCNTNNGCCNNNNNNATGATACGTTTTAGAACACAAACTATAGCAAAAGCAATGTAAATGGAANNAANTTTATTGATAAAAGAAAGATACANGTTTTAAGNGNTNTGCNTNGNGNCNCTTCNCNCCCCCTTACACCTTGTTTTCACTCANATACAAGATAAACCCTATCTCTCTCTTCNTATTTTCTTATATAGTACTATCTGCCCTCATCATCTCACAGAGCACCNTTACAGTNATAAGCAATGAGAATGTGACAGCTAATAACTATGATTNAACCTTNACTCTAGTANAGTTCACCTTAANTTCTAGAGTCTTCNTTTGGTTCCTTCTTCTTNCTCTTTCTNNTGTTNGGTCTTGGGCCTTCTTTTATATTGCNNTNGAATAGGNGGGTGNTGGGNCTCATACGTANNNNTNTNNNNNCTGNAGACCAGTCNATCTCATGTANAGTAGAGGCCAGCAATAGTGATCAGGTATTTGTTGTGTTTAAAGTTNTNTNTTTTTATCTCCANATGTNTATATATTGTTTTGTGTNTATGGATTTAACNAATCTAGACCAATGGATAAANNTTTTTTTTATNATNTTTTGTTTTGTTTTGATAGATGAATTNNGGCTCCTANTTCCAGTCTATCTCNGGCAAAGGTAGAGTAGAGGCCAGCACCAAAGATCCGGTATTTGTTGTGTTTAAAGTTTTATCTTTTTATCTCNAAANGTTTATATNTTAAACTTATATAAATGGTGAATCTGGATAGGTTGATGATCAAGGTCCATCAGATGAAATTNATTCCCATAATGGCCCCTTATATATNAATGATGAGGAGGGAGCGAATGAAACGGTTTGTCATTCTCAATAATGCAAATGAATTTGGTTTTGTAACATATATTCTAGGATATTAGGAAACTAAATATNAATAACTNGCAGGAAAGAAGCTATCCTCGACCTCTTCTTAAAAGAGGAAGAGACGGAATACTTAGAAGTGAAGGAAAATTAAAACAAAGGTTATATTTACTCCATCTTCTCGCACCAGAAAAATGCAGGGGAACAAGAAACAAAAATACCGCTTGATAGCCNCGATTTTGAAGGAAACGAAGCCAAAAGGAAAANAAAATACAAATCAACCTTCTTCAATAGTTTANTTTTCTTATGTTCTTGTTACGTTTTTATTCAGATNTTTATTCAGTTNAGAAACNAATTCNNTGGAGTCTTTNATTAGTTTTATCAATNNNNNNNNNNNNNNNNTANAATTTATAACTTCGTATCCAAATTCCTNAANCAGTGACTTGCCTGATGAATTGCTGGTGGAGATATTAACGTTTCTTCCAACAAAAGNTGTTGTNTACCTTAAACATGCAANTTCCTTTTACATCTACCAATCAATTTTGAGTGTCTGATGGGTGCATTGATCTCTATGGCATTATCAAAGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.061795
PRIMER_LEFT_0_PENALTY=4.049325
PRIMER_RIGHT_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_0_SEQUENCE=TTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_0=924,20
PRIMER_RIGHT_0=1836,20
PRIMER_LEFT_0_TM=55.951
PRIMER_RIGHT_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.1000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=913
PRIMER_PAIR_1_PENALTY=4.083172
PRIMER_LEFT_1_PENALTY=4.070702
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_1_SEQUENCE=TTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_1=922,22
PRIMER_RIGHT_1=1836,20
PRIMER_LEFT_1_TM=57.929
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=915
PRIMER_PAIR_2_PENALTY=4.094157
PRIMER_LEFT_2_PENALTY=4.049325
PRIMER_RIGHT_2_PENALTY=0.044832
PRIMER_LEFT_2_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_2_SEQUENCE=ATACCGGATCTTTGGTGCTG
PRIMER_LEFT_2=924,20
PRIMER_RIGHT_2=1847,20
PRIMER_LEFT_2_TM=55.951
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=924
PRIMER_PAIR_3_PENALTY=4.115533
PRIMER_LEFT_3_PENALTY=4.070702
PRIMER_RIGHT_3_PENALTY=0.044832
PRIMER_LEFT_3_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_3_SEQUENCE=ATACCGGATCTTTGGTGCTG
PRIMER_LEFT_3=922,22
PRIMER_RIGHT_3=1847,20
PRIMER_LEFT_3_TM=57.929
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=926
PRIMER_PAIR_4_PENALTY=4.446269
PRIMER_LEFT_4_PENALTY=4.049325
PRIMER_RIGHT_4_PENALTY=0.396944
PRIMER_LEFT_4_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_4_SEQUENCE=TTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_4=924,20
PRIMER_RIGHT_4=1837,20
PRIMER_LEFT_4_TM=55.951
PRIMER_RIGHT_4_TM=60.397
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=914
PRIMER_PAIR_5_PENALTY=4.467645
PRIMER_LEFT_5_PENALTY=4.070702
PRIMER_RIGHT_5_PENALTY=0.396944
PRIMER_LEFT_5_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_5_SEQUENCE=TTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_5=922,22
PRIMER_RIGHT_5=1837,20
PRIMER_LEFT_5_TM=57.929
PRIMER_RIGHT_5_TM=60.397
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=5.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=916
PRIMER_PAIR_6_PENALTY=4.553021
PRIMER_LEFT_6_PENALTY=4.049325
PRIMER_RIGHT_6_PENALTY=0.503696
PRIMER_LEFT_6_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_6_SEQUENCE=CTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_6=924,20
PRIMER_RIGHT_6=1838,20
PRIMER_LEFT_6_TM=55.951
PRIMER_RIGHT_6_TM=59.496
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=5.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=915
PRIMER_PAIR_7_PENALTY=4.556018
PRIMER_LEFT_7_PENALTY=4.543548
PRIMER_RIGHT_7_PENALTY=0.012470
PRIMER_LEFT_7_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_7_SEQUENCE=TTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_7=923,21
PRIMER_RIGHT_7=1836,20
PRIMER_LEFT_7_TM=56.456
PRIMER_RIGHT_7_TM=60.012
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=914
PRIMER_PAIR_8_PENALTY=4.574398
PRIMER_LEFT_8_PENALTY=4.070702
PRIMER_RIGHT_8_PENALTY=0.503696
PRIMER_LEFT_8_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_8_SEQUENCE=CTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_8=922,22
PRIMER_RIGHT_8=1838,20
PRIMER_LEFT_8_TM=57.929
PRIMER_RIGHT_8_TM=59.496
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=5.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=917
PRIMER_PAIR_9_PENALTY=4.588379
PRIMER_LEFT_9_PENALTY=4.543548
PRIMER_RIGHT_9_PENALTY=0.044832
PRIMER_LEFT_9_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_9_SEQUENCE=ATACCGGATCTTTGGTGCTG
PRIMER_LEFT_9=923,21
PRIMER_RIGHT_9=1847,20
PRIMER_LEFT_9_TM=56.456
PRIMER_RIGHT_9_TM=59.955
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=925
PRIMER_PAIR_10_PENALTY=4.940491
PRIMER_LEFT_10_PENALTY=4.543548
PRIMER_RIGHT_10_PENALTY=0.396944
PRIMER_LEFT_10_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_10_SEQUENCE=TTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_10=923,21
PRIMER_RIGHT_10=1837,20
PRIMER_LEFT_10_TM=56.456
PRIMER_RIGHT_10_TM=60.397
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=5.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=915
PRIMER_PAIR_11_PENALTY=5.047244
PRIMER_LEFT_11_PENALTY=4.543548
PRIMER_RIGHT_11_PENALTY=0.503696
PRIMER_LEFT_11_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_11_SEQUENCE=CTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_11=923,21
PRIMER_RIGHT_11=1838,20
PRIMER_LEFT_11_TM=56.456
PRIMER_RIGHT_11_TM=59.496
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=5.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=916
PRIMER_PAIR_12_PENALTY=5.200429
PRIMER_LEFT_12_PENALTY=4.049325
PRIMER_RIGHT_12_PENALTY=1.151103
PRIMER_LEFT_12_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_12_SEQUENCE=AAATACCGGATCTTTGGTGCT
PRIMER_LEFT_12=924,20
PRIMER_RIGHT_12=1849,21
PRIMER_LEFT_12_TM=55.951
PRIMER_RIGHT_12_TM=59.849
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=926
PRIMER_PAIR_13_PENALTY=5.221805
PRIMER_LEFT_13_PENALTY=4.070702
PRIMER_RIGHT_13_PENALTY=1.151103
PRIMER_LEFT_13_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_13_SEQUENCE=AAATACCGGATCTTTGGTGCT
PRIMER_LEFT_13=922,22
PRIMER_RIGHT_13=1849,21
PRIMER_LEFT_13_TM=57.929
PRIMER_RIGHT_13_TM=59.849
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=928
PRIMER_PAIR_14_PENALTY=5.519782
PRIMER_LEFT_14_PENALTY=4.049325
PRIMER_RIGHT_14_PENALTY=1.470456
PRIMER_LEFT_14_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_14_SEQUENCE=AATACCGGATCTTTGGTGCT
PRIMER_LEFT_14=924,20
PRIMER_RIGHT_14=1848,20
PRIMER_LEFT_14_TM=55.951
PRIMER_RIGHT_14_TM=58.530
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=925
PRIMER_PAIR_15_PENALTY=5.538619
PRIMER_LEFT_15_PENALTY=4.049325
PRIMER_RIGHT_15_PENALTY=1.489294
PRIMER_LEFT_15_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_15_SEQUENCE=TGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_15=924,20
PRIMER_RIGHT_15=1835,21
PRIMER_LEFT_15_TM=55.951
PRIMER_RIGHT_15_TM=59.511
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=57.143
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=5.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=912
PRIMER_PAIR_16_PENALTY=5.541158
PRIMER_LEFT_16_PENALTY=4.070702
PRIMER_RIGHT_16_PENALTY=1.470456
PRIMER_LEFT_16_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_16_SEQUENCE=AATACCGGATCTTTGGTGCT
PRIMER_LEFT_16=922,22
PRIMER_RIGHT_16=1848,20
PRIMER_LEFT_16_TM=57.929
PRIMER_RIGHT_16_TM=58.530
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=927
PRIMER_PAIR_17_PENALTY=5.559995
PRIMER_LEFT_17_PENALTY=4.070702
PRIMER_RIGHT_17_PENALTY=1.489294
PRIMER_LEFT_17_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_17_SEQUENCE=TGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_17=922,22
PRIMER_RIGHT_17=1835,21
PRIMER_LEFT_17_TM=57.929
PRIMER_RIGHT_17_TM=59.511
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=57.143
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=5.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=914
PRIMER_PAIR_18_PENALTY=5.694651
PRIMER_LEFT_18_PENALTY=4.543548
PRIMER_RIGHT_18_PENALTY=1.151103
PRIMER_LEFT_18_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_18_SEQUENCE=AAATACCGGATCTTTGGTGCT
PRIMER_LEFT_18=923,21
PRIMER_RIGHT_18=1849,21
PRIMER_LEFT_18_TM=56.456
PRIMER_RIGHT_18_TM=59.849
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=927
PRIMER_PAIR_19_PENALTY=5.751487
PRIMER_LEFT_19_PENALTY=4.049325
PRIMER_RIGHT_19_PENALTY=1.702162
PRIMER_LEFT_19_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_19_SEQUENCE=GATCTTTGGTGCTGGCCTCT
PRIMER_LEFT_19=924,20
PRIMER_RIGHT_19=1841,20
PRIMER_LEFT_19_TM=55.951
PRIMER_RIGHT_19_TM=61.702
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=918
PRIMER_PAIR_20_PENALTY=5.772863
PRIMER_LEFT_20_PENALTY=4.070702
PRIMER_RIGHT_20_PENALTY=1.702162
PRIMER_LEFT_20_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_20_SEQUENCE=GATCTTTGGTGCTGGCCTCT
PRIMER_LEFT_20=922,22
PRIMER_RIGHT_20=1841,20
PRIMER_LEFT_20_TM=57.929
PRIMER_RIGHT_20_TM=61.702
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=920
PRIMER_PAIR_21_PENALTY=6.014004
PRIMER_LEFT_21_PENALTY=4.543548
PRIMER_RIGHT_21_PENALTY=1.470456
PRIMER_LEFT_21_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_21_SEQUENCE=AATACCGGATCTTTGGTGCT
PRIMER_LEFT_21=923,21
PRIMER_RIGHT_21=1848,20
PRIMER_LEFT_21_TM=56.456
PRIMER_RIGHT_21_TM=58.530
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=926
PRIMER_PAIR_22_PENALTY=6.032841
PRIMER_LEFT_22_PENALTY=4.543548
PRIMER_RIGHT_22_PENALTY=1.489294
PRIMER_LEFT_22_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_22_SEQUENCE=TGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_22=923,21
PRIMER_RIGHT_22=1835,21
PRIMER_LEFT_22_TM=56.456
PRIMER_RIGHT_22_TM=59.511
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=57.143
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=5.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=913
PRIMER_PAIR_23_PENALTY=6.245709
PRIMER_LEFT_23_PENALTY=4.543548
PRIMER_RIGHT_23_PENALTY=1.702162
PRIMER_LEFT_23_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_23_SEQUENCE=GATCTTTGGTGCTGGCCTCT
PRIMER_LEFT_23=923,21
PRIMER_RIGHT_23=1841,20
PRIMER_LEFT_23_TM=56.456
PRIMER_RIGHT_23_TM=61.702
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=919
PRIMER_PAIR_24_PENALTY=6.281290
PRIMER_LEFT_24_PENALTY=4.049325
PRIMER_RIGHT_24_PENALTY=2.231965
PRIMER_LEFT_24_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_24_SEQUENCE=AATACCGGATCTTTGGTGCTG
PRIMER_LEFT_24=924,20
PRIMER_RIGHT_24=1848,21
PRIMER_LEFT_24_TM=55.951
PRIMER_RIGHT_24_TM=61.232
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=925
PRIMER_PAIR_25_PENALTY=6.302666
PRIMER_LEFT_25_PENALTY=4.070702
PRIMER_RIGHT_25_PENALTY=2.231965
PRIMER_LEFT_25_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_25_SEQUENCE=AATACCGGATCTTTGGTGCTG
PRIMER_LEFT_25=922,22
PRIMER_RIGHT_25=1848,21
PRIMER_LEFT_25_TM=57.929
PRIMER_RIGHT_25_TM=61.232
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=927
PRIMER_PAIR_26_PENALTY=6.365366
PRIMER_LEFT_26_PENALTY=4.049325
PRIMER_RIGHT_26_PENALTY=2.316040
PRIMER_LEFT_26_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_26_SEQUENCE=TTTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_26=924,20
PRIMER_RIGHT_26=1837,21
PRIMER_LEFT_26_TM=55.951
PRIMER_RIGHT_26_TM=61.316
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=914
PRIMER_PAIR_27_PENALTY=6.365366
PRIMER_LEFT_27_PENALTY=4.049325
PRIMER_RIGHT_27_PENALTY=2.316040
PRIMER_LEFT_27_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_27_SEQUENCE=CTTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_27=924,20
PRIMER_RIGHT_27=1838,21
PRIMER_LEFT_27_TM=55.951
PRIMER_RIGHT_27_TM=61.316
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=57.143
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=5.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=915
PRIMER_PAIR_28_PENALTY=6.365366
PRIMER_LEFT_28_PENALTY=4.049325
PRIMER_RIGHT_28_PENALTY=2.316040
PRIMER_LEFT_28_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_28_SEQUENCE=TCTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_28=924,20
PRIMER_RIGHT_28=1839,21
PRIMER_LEFT_28_TM=55.951
PRIMER_RIGHT_28_TM=61.316
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=5.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=916
PRIMER_PAIR_29_PENALTY=6.386742
PRIMER_LEFT_29_PENALTY=4.070702
PRIMER_RIGHT_29_PENALTY=2.316040
PRIMER_LEFT_29_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_29_SEQUENCE=TTTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_29=922,22
PRIMER_RIGHT_29=1837,21
PRIMER_LEFT_29_TM=57.929
PRIMER_RIGHT_29_TM=61.316
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.1000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=916
PRIMER_PAIR_30_PENALTY=6.386742
PRIMER_LEFT_30_PENALTY=4.070702
PRIMER_RIGHT_30_PENALTY=2.316040
PRIMER_LEFT_30_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_30_SEQUENCE=CTTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_30=922,22
PRIMER_RIGHT_30=1838,21
PRIMER_LEFT_30_TM=57.929
PRIMER_RIGHT_30_TM=61.316
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=57.143
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=5.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=917
PRIMER_PAIR_31_PENALTY=6.386742
PRIMER_LEFT_31_PENALTY=4.070702
PRIMER_RIGHT_31_PENALTY=2.316040
PRIMER_LEFT_31_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_31_SEQUENCE=TCTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_31=922,22
PRIMER_RIGHT_31=1839,21
PRIMER_LEFT_31_TM=57.929
PRIMER_RIGHT_31_TM=61.316
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=5.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=918
PRIMER_PAIR_32_PENALTY=6.484526
PRIMER_LEFT_32_PENALTY=4.049325
PRIMER_RIGHT_32_PENALTY=2.435201
PRIMER_LEFT_32_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_32_SEQUENCE=GCTGGCCTCTACTCTACCTTTG
PRIMER_LEFT_32=924,20
PRIMER_RIGHT_32=1831,22
PRIMER_LEFT_32_TM=55.951
PRIMER_RIGHT_32_TM=59.565
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=54.545
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=908
PRIMER_PAIR_33_PENALTY=6.505902
PRIMER_LEFT_33_PENALTY=4.070702
PRIMER_RIGHT_33_PENALTY=2.435201
PRIMER_LEFT_33_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_33_SEQUENCE=GCTGGCCTCTACTCTACCTTTG
PRIMER_LEFT_33=922,22
PRIMER_RIGHT_33=1831,22
PRIMER_LEFT_33_TM=57.929
PRIMER_RIGHT_33_TM=59.565
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=54.545
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.3000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=910
PRIMER_PAIR_34_PENALTY=6.775512
PRIMER_LEFT_34_PENALTY=4.543548
PRIMER_RIGHT_34_PENALTY=2.231965
PRIMER_LEFT_34_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_34_SEQUENCE=AATACCGGATCTTTGGTGCTG
PRIMER_LEFT_34=923,21
PRIMER_RIGHT_34=1848,21
PRIMER_LEFT_34_TM=56.456
PRIMER_RIGHT_34_TM=61.232
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=926
PRIMER_PAIR_35_PENALTY=6.809647
PRIMER_LEFT_35_PENALTY=4.049325
PRIMER_RIGHT_35_PENALTY=2.760322
PRIMER_LEFT_35_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_35_SEQUENCE=TGCTGGCCTCTACTCTACCTT
PRIMER_LEFT_35=924,20
PRIMER_RIGHT_35=1832,21
PRIMER_LEFT_35_TM=55.951
PRIMER_RIGHT_35_TM=58.240
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=909
PRIMER_PAIR_36_PENALTY=6.831023
PRIMER_LEFT_36_PENALTY=4.070702
PRIMER_RIGHT_36_PENALTY=2.760322
PRIMER_LEFT_36_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_36_SEQUENCE=TGCTGGCCTCTACTCTACCTT
PRIMER_LEFT_36=922,22
PRIMER_RIGHT_36=1832,21
PRIMER_LEFT_36_TM=57.929
PRIMER_RIGHT_36_TM=58.240
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=911
PRIMER_PAIR_37_PENALTY=6.859588
PRIMER_LEFT_37_PENALTY=4.543548
PRIMER_RIGHT_37_PENALTY=2.316040
PRIMER_LEFT_37_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_37_SEQUENCE=TTTGGTGCTGGCCTCTACTCT
PRIMER_LEFT_37=923,21
PRIMER_RIGHT_37=1837,21
PRIMER_LEFT_37_TM=56.456
PRIMER_RIGHT_37_TM=61.316
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=6.1000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=915
PRIMER_PAIR_38_PENALTY=6.859588
PRIMER_LEFT_38_PENALTY=4.543548
PRIMER_RIGHT_38_PENALTY=2.316040
PRIMER_LEFT_38_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_38_SEQUENCE=CTTTGGTGCTGGCCTCTACTC
PRIMER_LEFT_38=923,21
PRIMER_RIGHT_38=1838,21
PRIMER_LEFT_38_TM=56.456
PRIMER_RIGHT_38_TM=61.316
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=57.143
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=5.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=916
PRIMER_PAIR_39_PENALTY=6.859588
PRIMER_LEFT_39_PENALTY=4.543548
PRIMER_RIGHT_39_PENALTY=2.316040
PRIMER_LEFT_39_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_39_SEQUENCE=TCTTTGGTGCTGGCCTCTACT
PRIMER_LEFT_39=923,21
PRIMER_RIGHT_39=1839,21
PRIMER_LEFT_39_TM=56.456
PRIMER_RIGHT_39_TM=61.316
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=5.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=917
PRIMER_PAIR_40_PENALTY=6.860873
PRIMER_LEFT_40_PENALTY=4.049325
PRIMER_RIGHT_40_PENALTY=2.811547
PRIMER_LEFT_40_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_40_SEQUENCE=TTGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_40=924,20
PRIMER_RIGHT_40=1836,22
PRIMER_LEFT_40_TM=55.951
PRIMER_RIGHT_40_TM=60.812
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=54.545
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=5.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=913
PRIMER_PAIR_41_PENALTY=6.882249
PRIMER_LEFT_41_PENALTY=4.070702
PRIMER_RIGHT_41_PENALTY=2.811547
PRIMER_LEFT_41_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_41_SEQUENCE=TTGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_41=922,22
PRIMER_RIGHT_41=1836,22
PRIMER_LEFT_41_TM=57.929
PRIMER_RIGHT_41_TM=60.812
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=54.545
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=5.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=915
PRIMER_PAIR_42_PENALTY=6.968227
PRIMER_LEFT_42_PENALTY=4.049325
PRIMER_RIGHT_42_PENALTY=2.918902
PRIMER_LEFT_42_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_42_SEQUENCE=GTGCTGGCCTCTACTCTACCTT
PRIMER_LEFT_42=924,20
PRIMER_RIGHT_42=1833,22
PRIMER_LEFT_42_TM=55.951
PRIMER_RIGHT_42_TM=59.081
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=54.545
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=910
PRIMER_PAIR_43_PENALTY=6.978748
PRIMER_LEFT_43_PENALTY=4.543548
PRIMER_RIGHT_43_PENALTY=2.435201
PRIMER_LEFT_43_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_43_SEQUENCE=GCTGGCCTCTACTCTACCTTTG
PRIMER_LEFT_43=923,21
PRIMER_RIGHT_43=1831,22
PRIMER_LEFT_43_TM=56.456
PRIMER_RIGHT_43_TM=59.565
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=54.545
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.3000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=909
PRIMER_PAIR_44_PENALTY=6.989604
PRIMER_LEFT_44_PENALTY=4.070702
PRIMER_RIGHT_44_PENALTY=2.918902
PRIMER_LEFT_44_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_44_SEQUENCE=GTGCTGGCCTCTACTCTACCTT
PRIMER_LEFT_44=922,22
PRIMER_RIGHT_44=1833,22
PRIMER_LEFT_44_TM=57.929
PRIMER_RIGHT_44_TM=59.081
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=54.545
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=912
PRIMER_PAIR_45_PENALTY=7.293231
PRIMER_LEFT_45_PENALTY=4.049325
PRIMER_RIGHT_45_PENALTY=3.243906
PRIMER_LEFT_45_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_45_SEQUENCE=TACCGGATCTTTGGTGCTGG
PRIMER_LEFT_45=924,20
PRIMER_RIGHT_45=1846,20
PRIMER_LEFT_45_TM=55.951
PRIMER_RIGHT_45_TM=63.244
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=923
PRIMER_PAIR_46_PENALTY=7.303869
PRIMER_LEFT_46_PENALTY=4.543548
PRIMER_RIGHT_46_PENALTY=2.760322
PRIMER_LEFT_46_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_46_SEQUENCE=TGCTGGCCTCTACTCTACCTT
PRIMER_LEFT_46=923,21
PRIMER_RIGHT_46=1832,21
PRIMER_LEFT_46_TM=56.456
PRIMER_RIGHT_46_TM=58.240
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=910
PRIMER_PAIR_47_PENALTY=7.314608
PRIMER_LEFT_47_PENALTY=4.070702
PRIMER_RIGHT_47_PENALTY=3.243906
PRIMER_LEFT_47_SEQUENCE=AATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_47_SEQUENCE=TACCGGATCTTTGGTGCTGG
PRIMER_LEFT_47=922,22
PRIMER_RIGHT_47=1846,20
PRIMER_LEFT_47_TM=57.929
PRIMER_RIGHT_47_TM=63.244
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=925
PRIMER_PAIR_48_PENALTY=7.355095
PRIMER_LEFT_48_PENALTY=4.543548
PRIMER_RIGHT_48_PENALTY=2.811547
PRIMER_LEFT_48_SEQUENCE=ATGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_48_SEQUENCE=TTGGTGCTGGCCTCTACTCTAC
PRIMER_LEFT_48=923,21
PRIMER_RIGHT_48=1836,22
PRIMER_LEFT_48_TM=56.456
PRIMER_RIGHT_48_TM=60.812
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=54.545
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=5.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=914
PRIMER_PAIR_49_PENALTY=7.448946
PRIMER_LEFT_49_PENALTY=4.049325
PRIMER_RIGHT_49_PENALTY=3.399621
PRIMER_LEFT_49_SEQUENCE=TGGTGAATCTGGATAGGTTG
PRIMER_RIGHT_49_SEQUENCE=GTGCTGGCCTCTACTCTACC
PRIMER_LEFT_49=924,20
PRIMER_RIGHT_49=1833,20
PRIMER_LEFT_49_TM=55.951
PRIMER_RIGHT_49_TM=56.600
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=60.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=910
=
SEQUENCE_ID=MAGIC.149_2_4499837_4500261_ct-1_oy-0_89336_1559_TRUE
SEQUENCE_TARGET=950,524
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17718852_17719077_edi-0_oy-0_51031_46917_TRUE
SEQUENCE_TARGET=950,325
SEQUENCE_TEMPLATE=TCACGTGGACNGCCACAGCTTTCTTTATTATAATATTTGATAAAGTATTAATTCAGTTTGATTAAAGAATCTTCCTCTTAATAGTTTAATCCTAATCANAAACTTCGNAAACTATCAAATCAATAAAATATTAAGAACACACACGTTGAAAATTNCCCTTTCACTTATGATTCTCATAAACAAAACTAAGAAAAACAAACAATGTTCTCAATAGTCAATTCCCTAATAAGAGTCAAAAACCAAAGGCTTGACCCTCGTTGAACATGTTGCACATNCATCATTATATATCAGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACATAACTTTCACATTCAAAGAATTTTAAGCGACANCNCAAGTNTCAATGGAANCAACATCACAACAATTTATGTCTCAGAGTTACCTTAACGCTCAAGAAACCNCCACAAGGGCAACGAAAAATTACCTAACTTCGCTCCATTCGACACGTAAACAACCTTCAAAGCCCTTGAAGAGGCCTGCAANATCATCACCTTTGAATCCAATGCATCCTCATGTCTACCGAGTCGAGCCAGTAAACTTCAAAGNGTTGGTTCAGAGGCTAACCGGTGNACCTGAGCACGAGACTGTGCANGCTANNNCGCTCAAGACTTCGGATGATGCAGCAAAGCAGAGTTCTTCNTCATTTGCGTTTGATCCATCATCNTCTTGGNGAGATTTCTCATTCCAAAATCCTGCAAATATTTCCAGATGGTAGCTTCTACTCTTGCANNNNNNNNNNNNNNNNNNAAATNCTTAACATTCTTGTATTTANTTTTAGTTNNTTTTTNATAAACCAGAAAGATGTAATCANATATTTTTCAGACTCGCATTCTAGAATTAATTTGTTATGTCATTTATCTGTANNACACACATAATTTGTGGGAGTTAAGACTTTTGATTAAAATAAAACATAATTGGATTNATTAANGATTCTAACTACATTGGGAATAATTTGANATTTNCATTAAAAGAATAAATTGAATTAATCAATGATTCTATNTACACATGTTTAACATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANAATAANTTTACAGCTAATTAATGATGATTATTCTTATGTGTGTNGATATAATCAAGATGAGCCGTCAATGTTCTTCTCACCAAGCAATCTTCGTCGTCTTCTTTATCGCAGCTCTCTCCCGCCACCTCATGTANTTTTGATTCTATCATTTCCNNNTGAANCANAATGAAACATNNAATTACANACGAAAATATATAACTACGCATNAATCAACATTCAACATGTGTTTACGCATATAATACTAACCAGACTAAGTGTATCCGCCGGAGAGGTTGTGAAGGAGGCGAAGTAGGCAGGTTCAGGGCGGGCGNTGCACGTTAGCATGGTGGCGCAAAGGAGGAGAGCTATCGTGATCAAAGTAGAGAGATTTGCCATTTTGTGATTATAGATTAATTAATTATGGATATATTGAAAACTAATGACCAAAGCATCTTTATATATAGTCAATCNTGGTCAACTCTTTTCTTAATCTANTTCTTATTTTAAGAAGAAAAAAATTAGAGTGTTTTGTTCTTATCTTTTTCATTTGTTAATGTTNTTATCTATNAAAAATNTAGTTGTAAAAGTNAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAAATGAATGTGTAATATTCAAAATTTTNTAGTNTTTTNAAATTATATATAAANAAAAGTGGATTTGATAAAAGTTAGGTTATACCAATATGTTTCTAAAGATTAACCATCACNCATTACCTCNATTTATATTGGAAACATCTAGAAGNATATTTATAGGGAGGTTANATGGACTGANTNTTTTTTGCTACTACTGATTATTANTATTTTGCCATAATTTGCCACTNNTNNNGTTANGGGTAAAGGAGATTGNTAATTGGANCAAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTAAGAATAATNAAAGANNTTTTTTTGTTTGGCATNAGTCTATCAAAAACGTTGACCTNTTTTTACNTGAAGTCAACCNTTGATTTTTTCATATTGGGCCAAANATACCAATATACTGGCCCAATGNAATTCANNCATGATGGNGGTACAGTCGGAAANAAAAAACTTGAAANTTGAAACANNNNNTANTTTAACCCGATTTAATTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.397717
PRIMER_LEFT_0_PENALTY=0.009477
PRIMER_RIGHT_0_PENALTY=0.388240
PRIMER_LEFT_0_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_0_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_0=532,20
PRIMER_RIGHT_0=1384,20
PRIMER_LEFT_0_TM=60.009
PRIMER_RIGHT_0_TM=60.388
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=853
PRIMER_PAIR_1_PENALTY=0.613049
PRIMER_LEFT_1_PENALTY=0.224809
PRIMER_RIGHT_1_PENALTY=0.388240
PRIMER_LEFT_1_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_1_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_1=542,20
PRIMER_RIGHT_1=1384,20
PRIMER_LEFT_1_TM=60.225
PRIMER_RIGHT_1_TM=60.388
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=843
PRIMER_PAIR_2_PENALTY=0.642063
PRIMER_LEFT_2_PENALTY=0.253823
PRIMER_RIGHT_2_PENALTY=0.388240
PRIMER_LEFT_2_SEQUENCE=TGATGCAGCAAAGCAGAGTT
PRIMER_RIGHT_2_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_2=654,20
PRIMER_RIGHT_2=1384,20
PRIMER_LEFT_2_TM=59.746
PRIMER_RIGHT_2_TM=60.388
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=731
PRIMER_PAIR_3_PENALTY=0.651094
PRIMER_LEFT_3_PENALTY=0.262854
PRIMER_RIGHT_3_PENALTY=0.388240
PRIMER_LEFT_3_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_3_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_3=465,20
PRIMER_RIGHT_3=1384,20
PRIMER_LEFT_3_TM=60.263
PRIMER_RIGHT_3_TM=60.388
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=920
PRIMER_PAIR_4_PENALTY=1.162826
PRIMER_LEFT_4_PENALTY=0.009477
PRIMER_RIGHT_4_PENALTY=1.153349
PRIMER_LEFT_4_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_4_SEQUENCE=GCCCTGAACCTGCCTACTTC
PRIMER_LEFT_4=532,20
PRIMER_RIGHT_4=1396,20
PRIMER_LEFT_4_TM=60.009
PRIMER_RIGHT_4_TM=61.153
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=865
PRIMER_PAIR_5_PENALTY=1.181497
PRIMER_LEFT_5_PENALTY=0.009477
PRIMER_RIGHT_5_PENALTY=1.172020
PRIMER_LEFT_5_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_5_SEQUENCE=GCCTCCTTCACAACCTCTCC
PRIMER_LEFT_5=532,20
PRIMER_RIGHT_5=1376,20
PRIMER_LEFT_5_TM=60.009
PRIMER_RIGHT_5_TM=61.172
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=60.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=845
PRIMER_PAIR_6_PENALTY=1.296545
PRIMER_LEFT_6_PENALTY=0.009477
PRIMER_RIGHT_6_PENALTY=1.287068
PRIMER_LEFT_6_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_6_SEQUENCE=GCCTACTTCGCCTCCTTCAC
PRIMER_LEFT_6=532,20
PRIMER_RIGHT_6=1385,20
PRIMER_LEFT_6_TM=60.009
PRIMER_RIGHT_6_TM=61.287
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=854
PRIMER_PAIR_7_PENALTY=1.296545
PRIMER_LEFT_7_PENALTY=0.009477
PRIMER_RIGHT_7_PENALTY=1.287068
PRIMER_LEFT_7_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_7_SEQUENCE=GAACCTGCCTACTTCGCCTC
PRIMER_LEFT_7=532,20
PRIMER_RIGHT_7=1391,20
PRIMER_LEFT_7_TM=60.009
PRIMER_RIGHT_7_TM=61.287
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=60.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=860
PRIMER_PAIR_8_PENALTY=1.348638
PRIMER_LEFT_8_PENALTY=0.960398
PRIMER_RIGHT_8_PENALTY=0.388240
PRIMER_LEFT_8_SEQUENCE=TCCAATGCATCCTCATGTCT
PRIMER_RIGHT_8_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_8=537,20
PRIMER_RIGHT_8=1384,20
PRIMER_LEFT_8_TM=59.040
PRIMER_RIGHT_8_TM=60.388
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=848
PRIMER_PAIR_9_PENALTY=1.378158
PRIMER_LEFT_9_PENALTY=0.224809
PRIMER_RIGHT_9_PENALTY=1.153349
PRIMER_LEFT_9_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_9_SEQUENCE=GCCCTGAACCTGCCTACTTC
PRIMER_LEFT_9=542,20
PRIMER_RIGHT_9=1396,20
PRIMER_LEFT_9_TM=60.225
PRIMER_RIGHT_9_TM=61.153
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=60.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=855
PRIMER_PAIR_10_PENALTY=1.396829
PRIMER_LEFT_10_PENALTY=0.224809
PRIMER_RIGHT_10_PENALTY=1.172020
PRIMER_LEFT_10_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_10_SEQUENCE=GCCTCCTTCACAACCTCTCC
PRIMER_LEFT_10=542,20
PRIMER_RIGHT_10=1376,20
PRIMER_LEFT_10_TM=60.225
PRIMER_RIGHT_10_TM=61.172
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=60.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=835
PRIMER_PAIR_11_PENALTY=1.416203
PRIMER_LEFT_11_PENALTY=0.262854
PRIMER_RIGHT_11_PENALTY=1.153349
PRIMER_LEFT_11_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_11_SEQUENCE=GCCCTGAACCTGCCTACTTC
PRIMER_LEFT_11=465,20
PRIMER_RIGHT_11=1396,20
PRIMER_LEFT_11_TM=60.263
PRIMER_RIGHT_11_TM=61.153
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=60.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=932
PRIMER_PAIR_12_PENALTY=1.416933
PRIMER_LEFT_12_PENALTY=0.009477
PRIMER_RIGHT_12_PENALTY=1.407456
PRIMER_LEFT_12_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_12_SEQUENCE=CTGCCTACTTCGCCTCCTTC
PRIMER_LEFT_12=532,20
PRIMER_RIGHT_12=1387,20
PRIMER_LEFT_12_TM=60.009
PRIMER_RIGHT_12_TM=61.407
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=60.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=856
PRIMER_PAIR_13_PENALTY=1.425196
PRIMER_LEFT_13_PENALTY=1.036956
PRIMER_RIGHT_13_PENALTY=0.388240
PRIMER_LEFT_13_SEQUENCE=ATGATGCAGCAAAGCAGAGTT
PRIMER_RIGHT_13_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_13=653,21
PRIMER_RIGHT_13=1384,20
PRIMER_LEFT_13_TM=60.037
PRIMER_RIGHT_13_TM=60.388
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=732
PRIMER_PAIR_14_PENALTY=1.434874
PRIMER_LEFT_14_PENALTY=0.262854
PRIMER_RIGHT_14_PENALTY=1.172020
PRIMER_LEFT_14_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_14_SEQUENCE=GCCTCCTTCACAACCTCTCC
PRIMER_LEFT_14=465,20
PRIMER_RIGHT_14=1376,20
PRIMER_LEFT_14_TM=60.263
PRIMER_RIGHT_14_TM=61.172
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=60.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.1000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=912
PRIMER_PAIR_15_PENALTY=1.449687
PRIMER_LEFT_15_PENALTY=0.296338
PRIMER_RIGHT_15_PENALTY=1.153349
PRIMER_LEFT_15_SEQUENCE=GATGATGCAGCAAAGCAGAG
PRIMER_RIGHT_15_SEQUENCE=GCCCTGAACCTGCCTACTTC
PRIMER_LEFT_15=652,20
PRIMER_RIGHT_15=1396,20
PRIMER_LEFT_15_TM=59.704
PRIMER_RIGHT_15_TM=61.153
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=60.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=745
PRIMER_PAIR_16_PENALTY=1.511877
PRIMER_LEFT_16_PENALTY=0.224809
PRIMER_RIGHT_16_PENALTY=1.287068
PRIMER_LEFT_16_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_16_SEQUENCE=GCCTACTTCGCCTCCTTCAC
PRIMER_LEFT_16=542,20
PRIMER_RIGHT_16=1385,20
PRIMER_LEFT_16_TM=60.225
PRIMER_RIGHT_16_TM=61.287
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=60.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=844
PRIMER_PAIR_17_PENALTY=1.511877
PRIMER_LEFT_17_PENALTY=0.224809
PRIMER_RIGHT_17_PENALTY=1.287068
PRIMER_LEFT_17_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_17_SEQUENCE=GAACCTGCCTACTTCGCCTC
PRIMER_LEFT_17=542,20
PRIMER_RIGHT_17=1391,20
PRIMER_LEFT_17_TM=60.225
PRIMER_RIGHT_17_TM=61.287
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=60.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=850
PRIMER_PAIR_18_PENALTY=1.540891
PRIMER_LEFT_18_PENALTY=0.253823
PRIMER_RIGHT_18_PENALTY=1.287068
PRIMER_LEFT_18_SEQUENCE=TGATGCAGCAAAGCAGAGTT
PRIMER_RIGHT_18_SEQUENCE=GCCTACTTCGCCTCCTTCAC
PRIMER_LEFT_18=654,20
PRIMER_RIGHT_18=1385,20
PRIMER_LEFT_18_TM=59.746
PRIMER_RIGHT_18_TM=61.287
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=60.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=732
PRIMER_PAIR_19_PENALTY=1.549922
PRIMER_LEFT_19_PENALTY=0.262854
PRIMER_RIGHT_19_PENALTY=1.287068
PRIMER_LEFT_19_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_19_SEQUENCE=GCCTACTTCGCCTCCTTCAC
PRIMER_LEFT_19=465,20
PRIMER_RIGHT_19=1385,20
PRIMER_LEFT_19_TM=60.263
PRIMER_RIGHT_19_TM=61.287
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=60.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.1000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=921
PRIMER_PAIR_20_PENALTY=1.549922
PRIMER_LEFT_20_PENALTY=0.262854
PRIMER_RIGHT_20_PENALTY=1.287068
PRIMER_LEFT_20_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_20_SEQUENCE=GAACCTGCCTACTTCGCCTC
PRIMER_LEFT_20=465,20
PRIMER_RIGHT_20=1391,20
PRIMER_LEFT_20_TM=60.263
PRIMER_RIGHT_20_TM=61.287
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=60.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.1000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=927
PRIMER_PAIR_21_PENALTY=1.583406
PRIMER_LEFT_21_PENALTY=0.296338
PRIMER_RIGHT_21_PENALTY=1.287068
PRIMER_LEFT_21_SEQUENCE=GATGATGCAGCAAAGCAGAG
PRIMER_RIGHT_21_SEQUENCE=GAACCTGCCTACTTCGCCTC
PRIMER_LEFT_21=652,20
PRIMER_RIGHT_21=1391,20
PRIMER_LEFT_21_TM=59.704
PRIMER_RIGHT_21_TM=61.287
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=60.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=740
PRIMER_PAIR_22_PENALTY=1.599751
PRIMER_LEFT_22_PENALTY=0.009477
PRIMER_RIGHT_22_PENALTY=1.590274
PRIMER_LEFT_22_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_22_SEQUENCE=ACTTCGCCTCCTTCACAACC
PRIMER_LEFT_22=532,20
PRIMER_RIGHT_22=1381,20
PRIMER_LEFT_22_TM=60.009
PRIMER_RIGHT_22_TM=61.590
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=850
PRIMER_PAIR_23_PENALTY=1.617168
PRIMER_LEFT_23_PENALTY=1.228928
PRIMER_RIGHT_23_PENALTY=0.388240
PRIMER_LEFT_23_SEQUENCE=AATCCAATGCATCCTCATGTC
PRIMER_RIGHT_23_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_23=535,21
PRIMER_RIGHT_23=1384,20
PRIMER_LEFT_23_TM=59.771
PRIMER_RIGHT_23_TM=60.388
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.3000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=850
PRIMER_PAIR_24_PENALTY=1.632265
PRIMER_LEFT_24_PENALTY=0.224809
PRIMER_RIGHT_24_PENALTY=1.407456
PRIMER_LEFT_24_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_24_SEQUENCE=CTGCCTACTTCGCCTCCTTC
PRIMER_LEFT_24=542,20
PRIMER_RIGHT_24=1387,20
PRIMER_LEFT_24_TM=60.225
PRIMER_RIGHT_24_TM=61.407
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=60.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.6000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=846
PRIMER_PAIR_25_PENALTY=1.651845
PRIMER_LEFT_25_PENALTY=1.263605
PRIMER_RIGHT_25_PENALTY=0.388240
PRIMER_LEFT_25_SEQUENCE=TAACTTCGCTCCATTCGACAC
PRIMER_RIGHT_25_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_25=464,21
PRIMER_RIGHT_25=1384,20
PRIMER_LEFT_25_TM=60.264
PRIMER_RIGHT_25_TM=60.388
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.1000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=921
PRIMER_PAIR_26_PENALTY=1.665268
PRIMER_LEFT_26_PENALTY=1.277028
PRIMER_RIGHT_26_PENALTY=0.388240
PRIMER_LEFT_26_SEQUENCE=CCATTCGACACGTAAACAACC
PRIMER_RIGHT_26_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_26=474,21
PRIMER_RIGHT_26=1384,20
PRIMER_LEFT_26_TM=60.277
PRIMER_RIGHT_26_TM=60.388
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=911
PRIMER_PAIR_27_PENALTY=1.670310
PRIMER_LEFT_27_PENALTY=0.262854
PRIMER_RIGHT_27_PENALTY=1.407456
PRIMER_LEFT_27_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_27_SEQUENCE=CTGCCTACTTCGCCTCCTTC
PRIMER_LEFT_27=465,20
PRIMER_RIGHT_27=1387,20
PRIMER_LEFT_27_TM=60.263
PRIMER_RIGHT_27_TM=61.407
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=60.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.1000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=923
PRIMER_PAIR_28_PENALTY=1.720520
PRIMER_LEFT_28_PENALTY=0.009477
PRIMER_RIGHT_28_PENALTY=1.711043
PRIMER_LEFT_28_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_28_SEQUENCE=CTTCGCCTCCTTCACAACCT
PRIMER_LEFT_28=532,20
PRIMER_RIGHT_28=1380,20
PRIMER_LEFT_28_TM=60.009
PRIMER_RIGHT_28_TM=61.711
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=849
PRIMER_PAIR_29_PENALTY=1.761844
PRIMER_LEFT_29_PENALTY=1.373604
PRIMER_RIGHT_29_PENALTY=0.388240
PRIMER_LEFT_29_SEQUENCE=ATGATGCAGCAAAGCAGAGT
PRIMER_RIGHT_29_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_29=653,20
PRIMER_RIGHT_29=1384,20
PRIMER_LEFT_29_TM=58.626
PRIMER_RIGHT_29_TM=60.388
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.1000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=732
PRIMER_PAIR_30_PENALTY=1.815083
PRIMER_LEFT_30_PENALTY=0.224809
PRIMER_RIGHT_30_PENALTY=1.590274
PRIMER_LEFT_30_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_30_SEQUENCE=ACTTCGCCTCCTTCACAACC
PRIMER_LEFT_30=542,20
PRIMER_RIGHT_30=1381,20
PRIMER_LEFT_30_TM=60.225
PRIMER_RIGHT_30_TM=61.590
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=840
PRIMER_PAIR_31_PENALTY=1.844097
PRIMER_LEFT_31_PENALTY=0.253823
PRIMER_RIGHT_31_PENALTY=1.590274
PRIMER_LEFT_31_SEQUENCE=TGATGCAGCAAAGCAGAGTT
PRIMER_RIGHT_31_SEQUENCE=ACTTCGCCTCCTTCACAACC
PRIMER_LEFT_31=654,20
PRIMER_RIGHT_31=1381,20
PRIMER_LEFT_31_TM=59.746
PRIMER_RIGHT_31_TM=61.590
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=728
PRIMER_PAIR_32_PENALTY=1.853128
PRIMER_LEFT_32_PENALTY=0.262854
PRIMER_RIGHT_32_PENALTY=1.590274
PRIMER_LEFT_32_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_32_SEQUENCE=ACTTCGCCTCCTTCACAACC
PRIMER_LEFT_32=465,20
PRIMER_RIGHT_32=1381,20
PRIMER_LEFT_32_TM=60.263
PRIMER_RIGHT_32_TM=61.590
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.1000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=917
PRIMER_PAIR_33_PENALTY=1.860665
PRIMER_LEFT_33_PENALTY=1.472425
PRIMER_RIGHT_33_PENALTY=0.388240
PRIMER_LEFT_33_SEQUENCE=TACCGAGTCGAGCCAGTAAA
PRIMER_RIGHT_33_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_33=556,20
PRIMER_RIGHT_33=1384,20
PRIMER_LEFT_33_TM=58.528
PRIMER_RIGHT_33_TM=60.388
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.0000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=829
PRIMER_PAIR_34_PENALTY=1.869381
PRIMER_LEFT_34_PENALTY=1.481141
PRIMER_RIGHT_34_PENALTY=0.388240
PRIMER_LEFT_34_SEQUENCE=CTTTGAATCCAATGCATCCTC
PRIMER_RIGHT_34_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_34=530,21
PRIMER_RIGHT_34=1384,20
PRIMER_LEFT_34_TM=59.519
PRIMER_RIGHT_34_TM=60.388
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=855
PRIMER_PAIR_35_PENALTY=1.886356
PRIMER_LEFT_35_PENALTY=1.498116
PRIMER_RIGHT_35_PENALTY=0.388240
PRIMER_LEFT_35_SEQUENCE=ATGCATCCTCATGTCTACCGA
PRIMER_RIGHT_35_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_35=541,21
PRIMER_RIGHT_35=1384,20
PRIMER_LEFT_35_TM=60.498
PRIMER_RIGHT_35_TM=60.388
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.6000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=844
PRIMER_PAIR_36_PENALTY=1.886612
PRIMER_LEFT_36_PENALTY=0.296338
PRIMER_RIGHT_36_PENALTY=1.590274
PRIMER_LEFT_36_SEQUENCE=GATGATGCAGCAAAGCAGAG
PRIMER_RIGHT_36_SEQUENCE=ACTTCGCCTCCTTCACAACC
PRIMER_LEFT_36=652,20
PRIMER_RIGHT_36=1381,20
PRIMER_LEFT_36_TM=59.704
PRIMER_RIGHT_36_TM=61.590
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=730
PRIMER_PAIR_37_PENALTY=1.893872
PRIMER_LEFT_37_PENALTY=1.505632
PRIMER_RIGHT_37_PENALTY=0.388240
PRIMER_LEFT_37_SEQUENCE=TTTGAATCCAATGCATCCTC
PRIMER_RIGHT_37_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_37=531,20
PRIMER_RIGHT_37=1384,20
PRIMER_LEFT_37_TM=58.494
PRIMER_RIGHT_37_TM=60.388
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=854
PRIMER_PAIR_38_PENALTY=1.904948
PRIMER_LEFT_38_PENALTY=0.009477
PRIMER_RIGHT_38_PENALTY=1.895470
PRIMER_LEFT_38_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_38_SEQUENCE=CTACTTCGCCTCCTTCACAA
PRIMER_LEFT_38=532,20
PRIMER_RIGHT_38=1383,20
PRIMER_LEFT_38_TM=60.009
PRIMER_RIGHT_38_TM=58.105
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=852
PRIMER_PAIR_39_PENALTY=1.922383
PRIMER_LEFT_39_PENALTY=0.009477
PRIMER_RIGHT_39_PENALTY=1.912906
PRIMER_LEFT_39_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_39_SEQUENCE=CACGATAGCTCTCCTCCTTTG
PRIMER_LEFT_39=532,20
PRIMER_RIGHT_39=1443,21
PRIMER_LEFT_39_TM=60.009
PRIMER_RIGHT_39_TM=59.087
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=912
PRIMER_PAIR_40_PENALTY=1.935852
PRIMER_LEFT_40_PENALTY=0.224809
PRIMER_RIGHT_40_PENALTY=1.711043
PRIMER_LEFT_40_SEQUENCE=TGCATCCTCATGTCTACCGA
PRIMER_RIGHT_40_SEQUENCE=CTTCGCCTCCTTCACAACCT
PRIMER_LEFT_40=542,20
PRIMER_RIGHT_40=1380,20
PRIMER_LEFT_40_TM=60.225
PRIMER_RIGHT_40_TM=61.711
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=839
PRIMER_PAIR_41_PENALTY=1.951000
PRIMER_LEFT_41_PENALTY=1.562760
PRIMER_RIGHT_41_PENALTY=0.388240
PRIMER_LEFT_41_SEQUENCE=GATGATGCAGCAAAGCAGAGT
PRIMER_RIGHT_41_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_41=652,21
PRIMER_RIGHT_41=1384,20
PRIMER_LEFT_41_TM=60.563
PRIMER_RIGHT_41_TM=60.388
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.1000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=733
PRIMER_PAIR_42_PENALTY=1.964865
PRIMER_LEFT_42_PENALTY=0.253823
PRIMER_RIGHT_42_PENALTY=1.711043
PRIMER_LEFT_42_SEQUENCE=TGATGCAGCAAAGCAGAGTT
PRIMER_RIGHT_42_SEQUENCE=CTTCGCCTCCTTCACAACCT
PRIMER_LEFT_42=654,20
PRIMER_RIGHT_42=1380,20
PRIMER_LEFT_42_TM=59.746
PRIMER_RIGHT_42_TM=61.711
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=727
PRIMER_PAIR_43_PENALTY=1.973897
PRIMER_LEFT_43_PENALTY=0.262854
PRIMER_RIGHT_43_PENALTY=1.711043
PRIMER_LEFT_43_SEQUENCE=AACTTCGCTCCATTCGACAC
PRIMER_RIGHT_43_SEQUENCE=CTTCGCCTCCTTCACAACCT
PRIMER_LEFT_43=465,20
PRIMER_RIGHT_43=1380,20
PRIMER_LEFT_43_TM=60.263
PRIMER_RIGHT_43_TM=61.711
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.1000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=916
PRIMER_PAIR_44_PENALTY=2.000509
PRIMER_LEFT_44_PENALTY=1.612269
PRIMER_RIGHT_44_PENALTY=0.388240
PRIMER_LEFT_44_SEQUENCE=GCTCAAGACTTCGGATGATG
PRIMER_RIGHT_44_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_44=639,20
PRIMER_RIGHT_44=1384,20
PRIMER_LEFT_44_TM=58.388
PRIMER_RIGHT_44_TM=60.388
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=746
PRIMER_PAIR_45_PENALTY=2.007381
PRIMER_LEFT_45_PENALTY=0.296338
PRIMER_RIGHT_45_PENALTY=1.711043
PRIMER_LEFT_45_SEQUENCE=GATGATGCAGCAAAGCAGAG
PRIMER_RIGHT_45_SEQUENCE=CTTCGCCTCCTTCACAACCT
PRIMER_LEFT_45=652,20
PRIMER_RIGHT_45=1380,20
PRIMER_LEFT_45_TM=59.704
PRIMER_RIGHT_45_TM=61.711
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=729
PRIMER_PAIR_46_PENALTY=2.007727
PRIMER_LEFT_46_PENALTY=1.619488
PRIMER_RIGHT_46_PENALTY=0.388240
PRIMER_LEFT_46_SEQUENCE=ATCCAATGCATCCTCATGTCT
PRIMER_RIGHT_46_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_46=536,21
PRIMER_RIGHT_46=1384,20
PRIMER_LEFT_46_TM=59.381
PRIMER_RIGHT_46_TM=60.388
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=849
PRIMER_PAIR_47_PENALTY=2.015726
PRIMER_LEFT_47_PENALTY=0.009477
PRIMER_RIGHT_47_PENALTY=2.006249
PRIMER_LEFT_47_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_47_SEQUENCE=CTACTTCGCCTCCTTCACAAC
PRIMER_LEFT_47=532,20
PRIMER_RIGHT_47=1383,21
PRIMER_LEFT_47_TM=60.009
PRIMER_RIGHT_47_TM=58.994
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=852
PRIMER_PAIR_48_PENALTY=2.058238
PRIMER_LEFT_48_PENALTY=0.009477
PRIMER_RIGHT_48_PENALTY=2.048761
PRIMER_LEFT_48_SEQUENCE=TTGAATCCAATGCATCCTCA
PRIMER_RIGHT_48_SEQUENCE=TACTTCGCCTCCTTCACAAC
PRIMER_LEFT_48=532,20
PRIMER_RIGHT_48=1382,20
PRIMER_LEFT_48_TM=60.009
PRIMER_RIGHT_48_TM=57.951
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=851
PRIMER_PAIR_49_PENALTY=2.075854
PRIMER_LEFT_49_PENALTY=1.687614
PRIMER_RIGHT_49_PENALTY=0.388240
PRIMER_LEFT_49_SEQUENCE=CAAGGGCAACGAAAAATTACC
PRIMER_RIGHT_49_SEQUENCE=CCTACTTCGCCTCCTTCACA
PRIMER_LEFT_49=443,21
PRIMER_RIGHT_49=1384,20
PRIMER_LEFT_49_TM=60.688
PRIMER_RIGHT_49_TM=60.388
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.2000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=942
=
SEQUENCE_ID=MAGIC.287_3_9303805_9304536_ct-1_hi-0_288270_333_TRUE
SEQUENCE_TARGET=950,831
SEQUENCE_TEMPLATE=AGATCGAGATCGCTTTGNAGNTTTCGNGGAAAAGATTTGTATTGTATCGGATCAGAAGAAGACGGTTCNTCGTATTCNGGTGCTTTNTCCGCCGCGAGGAGCTCAAGTGTTAATGGCGGATTAGGGTTATGCGAAACAGAGTATTCTCGGAAGAGTAACTTCGAAGGAAGGAAGAGNAACTTCAGCGAGACGACGGAGNATNGGAAGAGTAATTTCGAAGGAGGAAGGAAGAGTAATTNCAGTGAGACNACGACGGAGAATCGGAAGAGTAATTTCAGTGAATCGGAGCCGCCGAGAAGAAGCGGTTTCGAGGCTAGGAAGAGTAATTTCAGTGAAACAGAGTATCCTACTAGGAGGAGTAATTTTAGTGAAACAGAGTATAATACTCGAAGAGGAAACAATCCGGCGACGGCGGAGAATCATCCACGGAGGAGTAGTAATTACGAAGCAGCGAGGAAAAGCGATTCGGCGGCGATGANTTTTACGAGAAGGGTAATGTCNATGAAAGAGAGNAGCTACTTCACCGGNGGAGAGGAGCCTGGTTTCATTGATCTTAAGTTTGATTCTTCCGGTGGAGGAGGAGGAGATGTAAACGACGGCGTTTTGGAACACGGAGGAGGAGGATCTTGTCGGAAAGATGGAGGAGAGTTGCGTAAGAGTCGAAAGAGTTTCAAAGGATGGAAGTGGATATTTGGACATCATCAACATCAACTTCATCATCATAACAGAGATTCATGATGAATTTATGAATTATTGAAGGAAAGTGACAATTTTTATTGGATCNAGTAATGAAACATGGAGCTGATTTGTGTGATCCGATGTNAATCTTAGNNTTTTNNTTCATATAATTATGGTTTATCATTTTGTTTTTTGANNNNNNNNNNNNNNNNATATATANNNNNNNNTAGCNAANTTTTGACGATTTTCGGTTTNTTTNTACTCGGTTTNTTANAANTTTTANGATTTTCNNTTNNTTTNCATTCGNTTTCTTAGATNTATNNTTTTANACATATAGTTTTCANAGCCAAAATAATACCGATNTANTATANATTAATTTGATATAGTTATCGGCTTTCGATTGGNTTCAATAATTTTTCATTTTTGGCTCGATTCGAGCTNAGTATCAGTTCTTTTGTAGAAANATAACACTATTGTTGTATATGTATTNAAGTTTGATTGAGTTTCACCACTGAATCTAATGTTTTAATCTACTTTCTAATTTGCTTAATGAATTGCTAATTTAGTTTTGTTCATAAACTTCTTGTTTCACCAATTCTTTATTTGTACCNTCTTGGNTAANAAATGTNTTACACAATGACTATGTAACTTTACATTTCAATAGATTTAGTTTCGTCGGGAAAAAAAGACGATATCAAGAATAAATATNTGTGAATGAGNTTTTGGGCCTACGNGCTTTCTTTATCTCTGCTTCAATTTGATTNACTAGTGGACTTNNTCAAGAGTTNTCATGCTACACTNAAATATGTTGCATGAATCTTTAACAAAAGANANGTTGATANAGCAGCAAAAAGGTGATAAAACTCTTGTATGAAAANTGNACATACTAGTGATTAAATCAATTTGCATCCAAACTNACANNNNNTTTTTTTNNGTNGTCGATCCAAACTCACATATATTGATAAAAGANCCACANTTNTACGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNTNNTNNNGNTTTNNNNNTNAANTTNNNNNNNCCNANANTNNAANATACNNTNNCTTTTNTNAANCNNANTANCTANAANNNAATNTNCNNTCNANNNAGCCCAANANNTANNANNNNANNNCCAATCAAGTNAGCNNAAGTNNCNATNACTNNNNCCCNNAANANNNCAAGNNTCANATGNNNCNAANNCNANNATNAANANNTCNNNANTNNNAANAAAANANNTNNNCANNGTCAACNANNNACNNNAGNGNCAANNTANNTANNTNTNNTGNNANNNTANANATANTNNANNNTTANNNTANNCNNAANNANNNNNTNTTTCATTTAGNNNTNANNNNAANATTNNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCGANACACNTTTTCTNTCCNNNNNNNNNNNNNNNNGTCTCCCACGATTTATNTGNTTCTATTTTTCCACTANNNNTTTNTTTCTGATTTGAGNGTTGGAGAANNGNAAGAAAACATNTTCCAATCATATATTTNTCTGCTCNTGCGTGTNNNTTGTGATGTAGGAGCCTTGTCTTCAATAGACAAACCGCACGACAAAAACTCCCCGAAACTTANNNTAATAATNTTGNATTTTNGTNAGTTGNTTATTAACANTCAAGTAANNNNNNATATTCGTTNAAANTGGTANATGATTTCTATTTNAAAAGTGCTTTTATGATATGATATAAAAGATTGTTATTTGATTTCTCTNACTATTGATTTATANNTATGATCCTATAAACATTTTAAGCATTTCCAAAATTTCATNAGGCTAGNGTTTNTTTTCTTACTTACCAATTTGTTTCTATATNTTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCCTCCATANTTAGGCATGGTCCTTNAAAAAAATGGTCAATGCATATTTTTGCCAAACGTATCANTGTGTCATCNTNTCTTCATGTTCCGATTCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_5472637_5472647_ler-0_tsu-0_113518_3993_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_17479622_17479985_sf-2_ws-0_131883_332228_TRUE
SEQUENCE_TARGET=950,463
SEQUENCE_TEMPLATE=NNNNNNNNNCTCAAAACAAAACAAAATTCGCATATCGCCGATTATTCAAATCGGTAACGTACGGTCTCATTTGAGATATTCCGGTGTGTGCAACAGNTACACCAATTAAACCTTTTCNTTAAAAAATANAAATGGAGAGATTNTTCTACAGAGAGAAACCCTAAAGACCAAAATAGGAATCGTGCCTATCACTCTCTTTCTCGAATCATCTTCTTCCTCGCGAAGAGACTTCCTTTAGTTTCTGTTGAAATCTCTTATTTGCGGTGATGAACTTTGAAATTTGATTCCAGAAGTCCGATGACGACGACGGCGGAAACTGGGCAATCCTCTGGTTCGAAACCCTCTGATGATGATGCATGGGCCAAACTGGGTCAGTCTNGTTTCCTTGTGGTGCTTACTCATTTCTCTAATTGATTGNAANTTCTCANTTTTAGTTCTAAATTGACGAATTCACTTNAATTTGAGATTTATTGTGAAACTTTCATAATGAATTCTATACAAATTTGCAGTTCCTCTGGACACCAGATTCTCTGACATTGAGATTAGGTGCNNTGATATGNTAATATGCTCTGAGATTAAACCNTCTTCCCTGGAAAAACATGAATGGTGCAGAATAACTAAGAATTTGGGTCAAAGTTCTGCNACTNTACATAATNAGAGGTATGNCAATAACNATTGACCTTCNCTAGCAAATGTCTTGTTATAGGTAGACAAAGTTCTTGTTAAGTTTATTAAGACTATTNAAAAGGNTTGGTCTTTCTAACTTGAATATGAAAGATCGTTTTATCGAACCNGCANAGGNTAGTTGTAACTAGCTTTTAGTATCACTCNTTTGGTAGCNGNNCATTTTGATTAAATNNNNNNNNNNNNNNNNNNNNNACAATNNTCTGTTTTCGGTTTCTTTGTAATNTTCTNCGTGTTGGTGTTTTGTTTTGTTAGTTCACCATCACATCTTTCTNTATTTGTATTTGTTTGCGCTTTCTGAANTGTGNNGNNNTTNNTTAGTTCGGATGCTATACTTGTTGACNAGGCTGTTGTCCCGAAAGANGGTGCTGTTGATATCATTAGTGGATCTGAAATTGTTCCTGGCCCTGAAGAACAAGGTTAGANCTGCTTACNTTTTTATCTCTCTGAGTTCTGAGTTTATTTCCTAAANCNTAATTGTTTTCATCTTGAAAATNGTATANACGATTGTACTAGTACAAGGGCAGCGNNNNNNNNNNTGAAAATAAATTTCTANATTTTNTNAATTTGAANTTGTNCAATNTGTTAGGGTACTTGCAGTACAGATTTACAATAATGCCTGCTCCAGAATCCAGAACCCAGCTATTACAGGTGCTCTANGATCTCTACTTATAGTCCANAATCTTACATTTTTGAAACTTTNTGTCGAATTTCTTATNTCTCTTTTCCAGATTTCCATAGATCCTGAACATGCAAAATGCAGTATCTGCTTAAACATATGGCATGATGTTGTCACAGCTGCACCTTGCCTTCATAATTTCTGGTCTGTCATNTGTTGATTTTGTTTTAGTTCTCATNNNNNNNNNNNNTATTTCACTNTATTGGTAGTTGATGNTTGATGTGTTCTGCTNTCAGTAATGGCTGTTTTTCAGAATGGATGCGGAGATCGGAGGAAAAACATAAACATGTGTTATGTCCACAGTGCAGAACAACAGTACAATATGTGGGAAAAAATCATTTTCTGAAGAACATACAAGAGGTATAATTATCTGTTTGCAAGCATTTTCGTCATTTACATTTTACATATGAATGTTCTTTCCATTTGCTACTCTTCTCCTANAGAATCTTTCTCATTCTTGTGCTTCATTTATGCTTGAGCTTTNGATGAATAATTCAGCTCTTATGTTAGGANATATTAAAAGTGGATGCTGCACTGAGGCGACCAGNTGAAGATATTGCAGTGTTGGACTCATCTGCATCGATACAATCAAATCTTGTAAGTATTTGCTGCAATATCTTCTATTAGGTAACTATTTTTGGAAATATNTAATGCCGTTATTTAACAAGATGAGNTTGTCTTGCTCCACATTGACTTCGTGGTGTAATGNCTAATAAATCATTACCATATCTTAAACACGCTAATGGTATTTCTGGTTCAACTAGTCNTGAGTTATATGTACCTGCCATGCTATCTNAATACTACAATTGACAACATTNTAGATCATTGGGAGCAAGAGAAAACGTCGATTGAATATGCCNGCGCCCACACATGAAGAAAGAGATAGCTTGCGTTTGCAATGCCCACAATGTGGTAATAGAAANTTATCTGGTTGCGTCTTAATATCTCTTTACNGTAAAGAANANCTCTCTATTNAGTGGATAGGCTACTTATCAACATT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.983620
PRIMER_LEFT_0_PENALTY=2.428785
PRIMER_RIGHT_0_PENALTY=0.554836
PRIMER_LEFT_0_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_0_SEQUENCE=TCAGTGCAGCATCCACTTTT
PRIMER_LEFT_0=915,20
PRIMER_RIGHT_0=1900,20
PRIMER_LEFT_0_TM=57.571
PRIMER_RIGHT_0_TM=59.445
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=986
PRIMER_PAIR_1_PENALTY=3.181837
PRIMER_LEFT_1_PENALTY=2.428785
PRIMER_RIGHT_1_PENALTY=0.753052
PRIMER_LEFT_1_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_1_SEQUENCE=TCCATTCTGAAAAACAGCCA
PRIMER_LEFT_1=915,20
PRIMER_RIGHT_1=1621,20
PRIMER_LEFT_1_TM=57.571
PRIMER_RIGHT_1_TM=59.247
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=707
PRIMER_PAIR_2_PENALTY=3.868722
PRIMER_LEFT_2_PENALTY=2.428785
PRIMER_RIGHT_2_PENALTY=1.439937
PRIMER_LEFT_2_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_2_SEQUENCE=CATCCATTCTGAAAAACAGCC
PRIMER_LEFT_2=915,20
PRIMER_RIGHT_2=1623,21
PRIMER_LEFT_2_TM=57.571
PRIMER_RIGHT_2_TM=59.560
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=709
PRIMER_PAIR_3_PENALTY=3.875435
PRIMER_LEFT_3_PENALTY=2.428785
PRIMER_RIGHT_3_PENALTY=1.446651
PRIMER_LEFT_3_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_3_SEQUENCE=CTCAGTGCAGCATCCACTTTT
PRIMER_LEFT_3=915,20
PRIMER_RIGHT_3=1901,21
PRIMER_LEFT_3_TM=57.571
PRIMER_RIGHT_3_TM=60.447
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=987
PRIMER_PAIR_4_PENALTY=4.389411
PRIMER_LEFT_4_PENALTY=2.428785
PRIMER_RIGHT_4_PENALTY=1.960627
PRIMER_LEFT_4_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_4_SEQUENCE=ATGTTTTTCCTCCGATCTCC
PRIMER_LEFT_4=915,20
PRIMER_RIGHT_4=1644,20
PRIMER_LEFT_4_TM=57.571
PRIMER_RIGHT_4_TM=58.039
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=730
PRIMER_PAIR_5_PENALTY=4.651668
PRIMER_LEFT_5_PENALTY=2.356567
PRIMER_RIGHT_5_PENALTY=2.295101
PRIMER_LEFT_5_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_5_SEQUENCE=CCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_5=584,20
PRIMER_RIGHT_5=1507,21
PRIMER_LEFT_5_TM=57.643
PRIMER_RIGHT_5_TM=58.705
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=924
PRIMER_PAIR_6_PENALTY=4.723886
PRIMER_LEFT_6_PENALTY=2.428785
PRIMER_RIGHT_6_PENALTY=2.295101
PRIMER_LEFT_6_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_6_SEQUENCE=CCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_6=915,20
PRIMER_RIGHT_6=1507,21
PRIMER_LEFT_6_TM=57.571
PRIMER_RIGHT_6_TM=58.705
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=593
PRIMER_PAIR_7_PENALTY=4.854907
PRIMER_LEFT_7_PENALTY=2.356567
PRIMER_RIGHT_7_PENALTY=2.498341
PRIMER_LEFT_7_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_7_SEQUENCE=CCAGAAATTATGAAGGCAAGGT
PRIMER_LEFT_7=584,20
PRIMER_RIGHT_7=1507,22
PRIMER_LEFT_7_TM=57.643
PRIMER_RIGHT_7_TM=59.502
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=924
PRIMER_PAIR_8_PENALTY=4.854907
PRIMER_LEFT_8_PENALTY=2.356567
PRIMER_RIGHT_8_PENALTY=2.498341
PRIMER_LEFT_8_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_8_SEQUENCE=ACCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_8=584,20
PRIMER_RIGHT_8=1508,22
PRIMER_LEFT_8_TM=57.643
PRIMER_RIGHT_8_TM=59.502
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=925
PRIMER_PAIR_9_PENALTY=4.927125
PRIMER_LEFT_9_PENALTY=2.428785
PRIMER_RIGHT_9_PENALTY=2.498341
PRIMER_LEFT_9_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_9_SEQUENCE=CCAGAAATTATGAAGGCAAGGT
PRIMER_LEFT_9=915,20
PRIMER_RIGHT_9=1507,22
PRIMER_LEFT_9_TM=57.571
PRIMER_RIGHT_9_TM=59.502
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=593
PRIMER_PAIR_10_PENALTY=4.927125
PRIMER_LEFT_10_PENALTY=2.428785
PRIMER_RIGHT_10_PENALTY=2.498341
PRIMER_LEFT_10_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_10_SEQUENCE=ACCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_10=915,20
PRIMER_RIGHT_10=1508,22
PRIMER_LEFT_10_TM=57.571
PRIMER_RIGHT_10_TM=59.502
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=594
PRIMER_PAIR_11_PENALTY=4.987214
PRIMER_LEFT_11_PENALTY=2.428785
PRIMER_RIGHT_11_PENALTY=2.558430
PRIMER_LEFT_11_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_11_SEQUENCE=TTATGTTTTTCCTCCGATCTCC
PRIMER_LEFT_11=915,20
PRIMER_RIGHT_11=1646,22
PRIMER_LEFT_11_TM=57.571
PRIMER_RIGHT_11_TM=59.442
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=732
PRIMER_PAIR_12_PENALTY=5.131772
PRIMER_LEFT_12_PENALTY=2.356567
PRIMER_RIGHT_12_PENALTY=2.775205
PRIMER_LEFT_12_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_12_SEQUENCE=CAGACCAGAAATTATGAAGGCA
PRIMER_LEFT_12=584,20
PRIMER_RIGHT_12=1511,22
PRIMER_LEFT_12_TM=57.643
PRIMER_RIGHT_12_TM=59.225
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=928
PRIMER_PAIR_13_PENALTY=5.203990
PRIMER_LEFT_13_PENALTY=2.428785
PRIMER_RIGHT_13_PENALTY=2.775205
PRIMER_LEFT_13_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_13_SEQUENCE=CAGACCAGAAATTATGAAGGCA
PRIMER_LEFT_13=915,20
PRIMER_RIGHT_13=1511,22
PRIMER_LEFT_13_TM=57.571
PRIMER_RIGHT_13_TM=59.225
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=597
PRIMER_PAIR_14_PENALTY=5.306015
PRIMER_LEFT_14_PENALTY=2.428785
PRIMER_RIGHT_14_PENALTY=2.877230
PRIMER_LEFT_14_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_14_SEQUENCE=TATGTTTTTCCTCCGATCTCC
PRIMER_LEFT_14=915,20
PRIMER_RIGHT_14=1645,21
PRIMER_LEFT_14_TM=57.571
PRIMER_RIGHT_14_TM=58.123
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=731
PRIMER_PAIR_15_PENALTY=5.592985
PRIMER_LEFT_15_PENALTY=2.356567
PRIMER_RIGHT_15_PENALTY=3.236418
PRIMER_LEFT_15_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_15_SEQUENCE=CAGAAATTATGAAGGCAAGGTG
PRIMER_LEFT_15=584,20
PRIMER_RIGHT_15=1506,22
PRIMER_LEFT_15_TM=57.643
PRIMER_RIGHT_15_TM=58.764
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=923
PRIMER_PAIR_16_PENALTY=5.665203
PRIMER_LEFT_16_PENALTY=2.428785
PRIMER_RIGHT_16_PENALTY=3.236418
PRIMER_LEFT_16_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_16_SEQUENCE=CAGAAATTATGAAGGCAAGGTG
PRIMER_LEFT_16=915,20
PRIMER_RIGHT_16=1506,22
PRIMER_LEFT_16_TM=57.571
PRIMER_RIGHT_16_TM=58.764
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=592
PRIMER_PAIR_17_PENALTY=5.709654
PRIMER_LEFT_17_PENALTY=2.428785
PRIMER_RIGHT_17_PENALTY=3.280870
PRIMER_LEFT_17_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_17_SEQUENCE=ATCCATTCTGAAAAACAGCC
PRIMER_LEFT_17=915,20
PRIMER_RIGHT_17=1622,20
PRIMER_LEFT_17_TM=57.571
PRIMER_RIGHT_17_TM=56.719
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=708
PRIMER_PAIR_18_PENALTY=6.425191
PRIMER_LEFT_18_PENALTY=4.130090
PRIMER_RIGHT_18_PENALTY=2.295101
PRIMER_LEFT_18_SEQUENCE=GGAAAAACATGAATGGTGCAGA
PRIMER_RIGHT_18_SEQUENCE=CCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_18=591,22
PRIMER_RIGHT_18=1507,21
PRIMER_LEFT_18_TM=62.130
PRIMER_RIGHT_18_TM=58.705
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=917
PRIMER_PAIR_19_PENALTY=6.468952
PRIMER_LEFT_19_PENALTY=2.356567
PRIMER_RIGHT_19_PENALTY=4.112386
PRIMER_LEFT_19_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_19_SEQUENCE=GACCAGAAATTATGAAGGCAAG
PRIMER_LEFT_19=584,20
PRIMER_RIGHT_19=1509,22
PRIMER_LEFT_19_TM=57.643
PRIMER_RIGHT_19_TM=57.888
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=926
PRIMER_PAIR_20_PENALTY=6.503187
PRIMER_LEFT_20_PENALTY=2.356567
PRIMER_RIGHT_20_PENALTY=4.146620
PRIMER_LEFT_20_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_20_SEQUENCE=GACCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_20=584,20
PRIMER_RIGHT_20=1509,23
PRIMER_LEFT_20_TM=57.643
PRIMER_RIGHT_20_TM=61.147
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=43.478
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=926
PRIMER_PAIR_21_PENALTY=6.541170
PRIMER_LEFT_21_PENALTY=2.428785
PRIMER_RIGHT_21_PENALTY=4.112386
PRIMER_LEFT_21_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_21_SEQUENCE=GACCAGAAATTATGAAGGCAAG
PRIMER_LEFT_21=915,20
PRIMER_RIGHT_21=1509,22
PRIMER_LEFT_21_TM=57.571
PRIMER_RIGHT_21_TM=57.888
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=595
PRIMER_PAIR_22_PENALTY=6.575405
PRIMER_LEFT_22_PENALTY=2.428785
PRIMER_RIGHT_22_PENALTY=4.146620
PRIMER_LEFT_22_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_22_SEQUENCE=GACCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_22=915,20
PRIMER_RIGHT_22=1509,23
PRIMER_LEFT_22_TM=57.571
PRIMER_RIGHT_22_TM=61.147
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=43.478
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.6000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=595
PRIMER_PAIR_23_PENALTY=6.628430
PRIMER_LEFT_23_PENALTY=4.130090
PRIMER_RIGHT_23_PENALTY=2.498341
PRIMER_LEFT_23_SEQUENCE=GGAAAAACATGAATGGTGCAGA
PRIMER_RIGHT_23_SEQUENCE=CCAGAAATTATGAAGGCAAGGT
PRIMER_LEFT_23=591,22
PRIMER_RIGHT_23=1507,22
PRIMER_LEFT_23_TM=62.130
PRIMER_RIGHT_23_TM=59.502
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=917
PRIMER_PAIR_24_PENALTY=6.628430
PRIMER_LEFT_24_PENALTY=4.130090
PRIMER_RIGHT_24_PENALTY=2.498341
PRIMER_LEFT_24_SEQUENCE=GGAAAAACATGAATGGTGCAGA
PRIMER_RIGHT_24_SEQUENCE=ACCAGAAATTATGAAGGCAAGG
PRIMER_LEFT_24=591,22
PRIMER_RIGHT_24=1508,22
PRIMER_LEFT_24_TM=62.130
PRIMER_RIGHT_24_TM=59.502
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=918
PRIMER_PAIR_25_PENALTY=6.669939
PRIMER_LEFT_25_PENALTY=2.428785
PRIMER_RIGHT_25_PENALTY=4.241155
PRIMER_LEFT_25_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_25_SEQUENCE=CATCCATTCTGAAAAACAGCCA
PRIMER_LEFT_25=915,20
PRIMER_RIGHT_25=1623,22
PRIMER_LEFT_25_TM=57.571
PRIMER_RIGHT_25_TM=62.241
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=7.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=709
PRIMER_PAIR_26_PENALTY=6.831649
PRIMER_LEFT_26_PENALTY=2.428785
PRIMER_RIGHT_26_PENALTY=4.402864
PRIMER_LEFT_26_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_26_SEQUENCE=GGAGAAGAGTAGCAAATGGAAAG
PRIMER_LEFT_26=915,20
PRIMER_RIGHT_26=1800,23
PRIMER_LEFT_26_TM=57.571
PRIMER_RIGHT_26_TM=58.597
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=886
PRIMER_PAIR_27_PENALTY=6.905295
PRIMER_LEFT_27_PENALTY=4.130090
PRIMER_RIGHT_27_PENALTY=2.775205
PRIMER_LEFT_27_SEQUENCE=GGAAAAACATGAATGGTGCAGA
PRIMER_RIGHT_27_SEQUENCE=CAGACCAGAAATTATGAAGGCA
PRIMER_LEFT_27=591,22
PRIMER_RIGHT_27=1511,22
PRIMER_LEFT_27_TM=62.130
PRIMER_RIGHT_27_TM=59.225
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=921
PRIMER_PAIR_28_PENALTY=6.947668
PRIMER_LEFT_28_PENALTY=2.428785
PRIMER_RIGHT_28_PENALTY=4.518883
PRIMER_LEFT_28_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_28_SEQUENCE=AGGAGAAGAGTAGCAAATGGAAAG
PRIMER_LEFT_28=915,20
PRIMER_RIGHT_28=1801,24
PRIMER_LEFT_28_TM=57.571
PRIMER_RIGHT_28_TM=59.481
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=41.667
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=887
PRIMER_PAIR_29_PENALTY=7.051510
PRIMER_LEFT_29_PENALTY=2.428785
PRIMER_RIGHT_29_PENALTY=4.622726
PRIMER_LEFT_29_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_29_SEQUENCE=CCGATCTCCGCATCCATTCT
PRIMER_LEFT_29=915,20
PRIMER_RIGHT_29=1633,20
PRIMER_LEFT_29_TM=57.571
PRIMER_RIGHT_29_TM=64.623
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=719
PRIMER_PAIR_30_PENALTY=7.051510
PRIMER_LEFT_30_PENALTY=2.428785
PRIMER_RIGHT_30_PENALTY=4.622726
PRIMER_LEFT_30_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_30_SEQUENCE=CTCCGATCTCCGCATCCATT
PRIMER_LEFT_30=915,20
PRIMER_RIGHT_30=1635,20
PRIMER_LEFT_30_TM=57.571
PRIMER_RIGHT_30_TM=64.623
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=721
PRIMER_PAIR_31_PENALTY=7.057800
PRIMER_LEFT_31_PENALTY=2.356567
PRIMER_RIGHT_31_PENALTY=4.701233
PRIMER_LEFT_31_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_31_SEQUENCE=GACCAGAAATTATGAAGGCA
PRIMER_LEFT_31=584,20
PRIMER_RIGHT_31=1509,20
PRIMER_LEFT_31_TM=57.643
PRIMER_RIGHT_31_TM=55.299
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=926
PRIMER_PAIR_32_PENALTY=7.130018
PRIMER_LEFT_32_PENALTY=2.428785
PRIMER_RIGHT_32_PENALTY=4.701233
PRIMER_LEFT_32_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_32_SEQUENCE=GACCAGAAATTATGAAGGCA
PRIMER_LEFT_32=915,20
PRIMER_RIGHT_32=1509,20
PRIMER_LEFT_32_TM=57.571
PRIMER_RIGHT_32_TM=55.299
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=9.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=595
PRIMER_PAIR_33_PENALTY=7.161514
PRIMER_LEFT_33_PENALTY=2.428785
PRIMER_RIGHT_33_PENALTY=4.732730
PRIMER_LEFT_33_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_33_SEQUENCE=TTTCCTCCGATCTCCGCATC
PRIMER_LEFT_33=915,20
PRIMER_RIGHT_33=1639,20
PRIMER_LEFT_33_TM=57.571
PRIMER_RIGHT_33_TM=64.733
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.6000
PRIMER_RIGHT_33_END_STABILITY=8.1000
PRIMER_PAIR_33_COMPL_ANY=2.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=725
PRIMER_PAIR_34_PENALTY=7.165112
PRIMER_LEFT_34_PENALTY=2.428785
PRIMER_RIGHT_34_PENALTY=4.736327
PRIMER_LEFT_34_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_34_SEQUENCE=AGGAGAAGAGTAGCAAATGGAA
PRIMER_LEFT_34=915,20
PRIMER_RIGHT_34=1801,22
PRIMER_LEFT_34_TM=57.571
PRIMER_RIGHT_34_TM=57.264
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.6000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=887
PRIMER_PAIR_35_PENALTY=7.242185
PRIMER_LEFT_35_PENALTY=2.428785
PRIMER_RIGHT_35_PENALTY=4.813401
PRIMER_LEFT_35_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_35_SEQUENCE=GGAGAAGAGTAGCAAATGGAA
PRIMER_LEFT_35=915,20
PRIMER_RIGHT_35=1800,21
PRIMER_LEFT_35_TM=57.571
PRIMER_RIGHT_35_TM=56.187
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.6000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=886
PRIMER_PAIR_36_PENALTY=7.286709
PRIMER_LEFT_36_PENALTY=2.356567
PRIMER_RIGHT_36_PENALTY=4.930143
PRIMER_LEFT_36_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_36_SEQUENCE=CAGAAATTATGAAGGCAAGG
PRIMER_LEFT_36=584,20
PRIMER_RIGHT_36=1506,20
PRIMER_LEFT_36_TM=57.643
PRIMER_RIGHT_36_TM=55.070
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=923
PRIMER_PAIR_37_PENALTY=7.286709
PRIMER_LEFT_37_PENALTY=2.356567
PRIMER_RIGHT_37_PENALTY=4.930143
PRIMER_LEFT_37_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_37_SEQUENCE=CCAGAAATTATGAAGGCAAG
PRIMER_LEFT_37=584,20
PRIMER_RIGHT_37=1507,20
PRIMER_LEFT_37_TM=57.643
PRIMER_RIGHT_37_TM=55.070
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=924
PRIMER_PAIR_38_PENALTY=7.336159
PRIMER_LEFT_38_PENALTY=2.356567
PRIMER_RIGHT_38_PENALTY=4.979593
PRIMER_LEFT_38_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_38_SEQUENCE=CCAGAAATTATGAAGGCAAGGTG
PRIMER_LEFT_38=584,20
PRIMER_RIGHT_38=1507,23
PRIMER_LEFT_38_TM=57.643
PRIMER_RIGHT_38_TM=61.980
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=43.478
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=924
PRIMER_PAIR_39_PENALTY=7.358927
PRIMER_LEFT_39_PENALTY=2.428785
PRIMER_RIGHT_39_PENALTY=4.930143
PRIMER_LEFT_39_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_39_SEQUENCE=CAGAAATTATGAAGGCAAGG
PRIMER_LEFT_39=915,20
PRIMER_RIGHT_39=1506,20
PRIMER_LEFT_39_TM=57.571
PRIMER_RIGHT_39_TM=55.070
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=592
PRIMER_PAIR_40_PENALTY=7.358927
PRIMER_LEFT_40_PENALTY=2.428785
PRIMER_RIGHT_40_PENALTY=4.930143
PRIMER_LEFT_40_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_40_SEQUENCE=CCAGAAATTATGAAGGCAAG
PRIMER_LEFT_40=915,20
PRIMER_RIGHT_40=1507,20
PRIMER_LEFT_40_TM=57.571
PRIMER_RIGHT_40_TM=55.070
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=593
PRIMER_PAIR_41_PENALTY=7.366508
PRIMER_LEFT_41_PENALTY=4.130090
PRIMER_RIGHT_41_PENALTY=3.236418
PRIMER_LEFT_41_SEQUENCE=GGAAAAACATGAATGGTGCAGA
PRIMER_RIGHT_41_SEQUENCE=CAGAAATTATGAAGGCAAGGTG
PRIMER_LEFT_41=591,22
PRIMER_RIGHT_41=1506,22
PRIMER_LEFT_41_TM=62.130
PRIMER_RIGHT_41_TM=58.764
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=916
PRIMER_PAIR_42_PENALTY=7.408377
PRIMER_LEFT_42_PENALTY=2.428785
PRIMER_RIGHT_42_PENALTY=4.979593
PRIMER_LEFT_42_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_42_SEQUENCE=CCAGAAATTATGAAGGCAAGGTG
PRIMER_LEFT_42=915,20
PRIMER_RIGHT_42=1507,23
PRIMER_LEFT_42_TM=57.571
PRIMER_RIGHT_42_TM=61.980
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=593
PRIMER_PAIR_43_PENALTY=7.460784
PRIMER_LEFT_43_PENALTY=2.428785
PRIMER_RIGHT_43_PENALTY=5.031999
PRIMER_LEFT_43_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_43_SEQUENCE=GCATCCATTCTGAAAAACAGCC
PRIMER_LEFT_43=915,20
PRIMER_RIGHT_43=1624,22
PRIMER_LEFT_43_TM=57.571
PRIMER_RIGHT_43_TM=63.032
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=710
PRIMER_PAIR_44_PENALTY=7.636917
PRIMER_LEFT_44_PENALTY=2.428785
PRIMER_RIGHT_44_PENALTY=5.208132
PRIMER_LEFT_44_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_44_SEQUENCE=TCCCACATATTGTACTGTTGTTCTG
PRIMER_LEFT_44=915,20
PRIMER_RIGHT_44=1692,25
PRIMER_LEFT_44_TM=57.571
PRIMER_RIGHT_44_TM=60.208
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=778
PRIMER_PAIR_45_PENALTY=7.643793
PRIMER_LEFT_45_PENALTY=2.428785
PRIMER_RIGHT_45_PENALTY=5.215009
PRIMER_LEFT_45_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_45_SEQUENCE=AGGAGAAGAGTAGCAAATGGA
PRIMER_LEFT_45=915,20
PRIMER_RIGHT_45=1801,21
PRIMER_LEFT_45_TM=57.571
PRIMER_RIGHT_45_TM=55.785
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=8.1000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=887
PRIMER_PAIR_46_PENALTY=7.648289
PRIMER_LEFT_46_PENALTY=2.356567
PRIMER_RIGHT_46_PENALTY=5.291723
PRIMER_LEFT_46_SEQUENCE=CTTCCCTGGAAAAACATGAA
PRIMER_RIGHT_46_SEQUENCE=CAGACCAGAAATTATGAAGGCAAG
PRIMER_LEFT_46=584,20
PRIMER_RIGHT_46=1511,24
PRIMER_LEFT_46_TM=57.643
PRIMER_RIGHT_46_TM=61.292
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=41.667
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=928
PRIMER_PAIR_47_PENALTY=7.720507
PRIMER_LEFT_47_PENALTY=2.428785
PRIMER_RIGHT_47_PENALTY=5.291723
PRIMER_LEFT_47_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_47_SEQUENCE=CAGACCAGAAATTATGAAGGCAAG
PRIMER_LEFT_47=915,20
PRIMER_RIGHT_47=1511,24
PRIMER_LEFT_47_TM=57.571
PRIMER_RIGHT_47_TM=61.292
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=41.667
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=597
PRIMER_PAIR_48_PENALTY=7.740110
PRIMER_LEFT_48_PENALTY=2.428785
PRIMER_RIGHT_48_PENALTY=5.311325
PRIMER_LEFT_48_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_48_SEQUENCE=GTTTATGTTTTTCCTCCGATCTCC
PRIMER_LEFT_48=915,20
PRIMER_RIGHT_48=1648,24
PRIMER_LEFT_48_TM=57.571
PRIMER_RIGHT_48_TM=61.311
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=41.667
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=2.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=734
PRIMER_PAIR_49_PENALTY=7.921943
PRIMER_LEFT_49_PENALTY=2.428785
PRIMER_RIGHT_49_PENALTY=5.493158
PRIMER_LEFT_49_SEQUENCE=CGTGTTGGTGTTTTGTTTTG
PRIMER_RIGHT_49_SEQUENCE=TAGGAGAAGAGTAGCAAATGGAAAG
PRIMER_LEFT_49=915,20
PRIMER_RIGHT_49=1802,25
PRIMER_LEFT_49_TM=57.571
PRIMER_RIGHT_49_TM=59.507
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=888
=
SEQUENCE_ID=MAGIC.446_2_6244979_6245650_po-0_wu-0_39476_17200_TRUE
SEQUENCE_TARGET=950,771
SEQUENCE_TEMPLATE=TTGCAACTAACAATTACNTNTTTTTTTTNTCATCACACTATAGAAAAGCTTTNAAAAAGATAGTTGTCTNCTTTCGNAAAAACACGAAACCAATGTAAATTAGAAGATAGTTCGCATATTGATTTGGAAAANTCAGTCCTAAGATTGATGAAGCAAATTAGAGTGAGTGTTTCATGNNNNNNAGNCANTTTTTGGAAGCTTTTCTTTGGATTGAATAAATCCCCACGNGATGATATGTTATCTCTCTGCATGAAAGTAATGAATAAACCTTTTCAACAATCTATTAACAATGCAAAGACTTCTAAGCAAGAAAAGTCGTATAAGTTTTTTCTTTTTGGTANANTTTAGTTTAAAATCGTGTCCGTTGTTTATTTGCCAGATGNCATTTTCTNTTAGATTTAAAATATGTCAGCAATCTATTTAACTTTAANGCGAACGGATAAAATGAGATTTTAGCGGTTAACTTGATCAAAATCTAATAAAAAGTTACAAACTAAAATGAATGATAAATGAAGGTTACATGCATGGAGAAATTTTTTACTTTGTAAATGACTTGCTGCCTAATAAAAATTAGTTATTTTTAATATTTAAAAGNAAACATNATATGTAAGTTAATAAGTATGAATTGTAAGGAAGCAAACATTCTTACGTTTTGAAAAANGAAAACAAAAAGATTCCTACGTAAGTATGTATTNTCTTTGCTTCTCCACAAAGATATTCTTTGTATAGGACACAATTTACTTTAAACACACAATACAATATGTATATNTACTAATCAATGATCGTTATATATATTCTTTGTATAGGACACAATTTACTTTAAACACTTTTCATGCAATACAATATGTATATATACTAATCAATGATCGTTATATATGTCCATCGTACTCAACCTCAAACATCTTAAATAATGACTCTAATAAANCGNTCAAAACCATTTGTACTTCTCNTANGCTTCTNAANTATCTCAAGTTTGCTATTATGGGTCTCACAAGCTTCCNTTGTTTCATCAGNAGATTTTAACAAAGACTTCTTTGTGACATGGTCTCCTACCCACGTTAACACCTCCAATGATGGTCGATCAAGAACCCTNANACTCGATCAAGAATCTGGTATTTTCATATANANGTCCAAATGTCCTNGTTNTATTTTTCNGCATNNAGAACTTATGTTTTGATAATATTAGGAGCTTCTTTTTCTTCCATNCAGACGTTCTTGTTCGGCCAAATAGATATGAAGATCAAACTTATCCNTNGAAGTTCTCAAGGNACCGTGGTTGCTTATTATGTAAGAAACNGTCTCTATCTCTTCATCTTAACGGGTTGATGTGAATGAATCTCATAATTTCCANGATTTATCAGATGTCCTCAGATCAGCCNAACCGGGATGAGATTGACTTTGANTTCCTCGGAAACGTTANTGGGCAGCCTTACATTCTCCAGACAAATGTCTATGCCGANGGANTTGATAACCGCGAAGAAAGGATCCATCTCTGGTTCGACCCANCCAAGGACTTTCACACTTACTCTATCTTGTGGAACATTCACCAAATTGTGTAAGANTNNTCAACCAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGGTGGTTTCAGATTTATGGTGGATCAGATTCCNATAANNTTGTATAGAAACCANGNGGANAAAGGCGTAGCCTACCCAANGTTGCAACCTATGAGTGTACAGGCGAGTCTATGGAANGGTGAGAGCTGGGCTACNNGCGGTGGNCNTGACAAGATTGATTGGTCAAAGGGTCCATTTGTNGCTTNCTTTNGGGATTACAANNTAGACGCTTGTATTTGGANNGGCAATNCAAGTTTTTGTAATGGAGAGAGCACTGAAAACTGGTGGAACAAGAATGAGTTCAGTTCTTTGACAAGAGTGCAAAAGAGATGGTTTAAATGGGTGAGNAAATATCACTTGATTTATGATTATTGCCAAGATTATGGAAGGTTCANTAACAAGCTACCGAAGGAATGTTCTCTTCCTAAATATTNAATTTGCTCAAATCTTNGGTTCNCCTCCTTTTCTNTTAGCCAATAACATTATGCCATGTTANACTGAATTTAAATAAAAATAATTAANTAAAANTAAATCAGAAAAACCAAAAATGGAAAGAAAATNCTTNTATNTATTTAANGTACCTTAACGTCAAGGTTGACATCTTCTCCTNNNNTTNNNNNNTNTAATGTAANTNTTTTAATATGAGCAAATTTGAAAGAANGACNATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGNANGNCACTATGATTGGGGTAANCGAGATNCCGNGGATCTATCATCTCCCACGATCTTAANCGTTCATTTTNTGGCATGAACNATTANATCTCNAAGGGATAATAATTANNNATCCCTTNTGTAAATCACTATCNTGGCTTCATNTATNGGTATTAGTGNACAAGGTTTTGCGGANTGTCATTTGATTCANCTNAGNTAAACTTGCTTTCACANNTAAATAATCANCTCCTAAACAATTTTTTGTTTTGCAATAATCTCAGGNAACAAAGACATGTTTNAAACCATACANTCNTACCATGCANTCAAGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.158800
PRIMER_LEFT_0_PENALTY=1.850020
PRIMER_RIGHT_0_PENALTY=1.308781
PRIMER_LEFT_0_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_0_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_0=876,21
PRIMER_RIGHT_0=1793,20
PRIMER_LEFT_0_TM=59.150
PRIMER_RIGHT_0_TM=58.691
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=918
PRIMER_PAIR_1_PENALTY=3.339057
PRIMER_LEFT_1_PENALTY=2.030276
PRIMER_RIGHT_1_PENALTY=1.308781
PRIMER_LEFT_1_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_1_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_1=879,22
PRIMER_RIGHT_1=1793,20
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=58.691
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=915
PRIMER_PAIR_2_PENALTY=3.402142
PRIMER_LEFT_2_PENALTY=1.850020
PRIMER_RIGHT_2_PENALTY=1.552122
PRIMER_LEFT_2_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_2_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_2=876,21
PRIMER_RIGHT_2=1790,21
PRIMER_LEFT_2_TM=59.150
PRIMER_RIGHT_2_TM=60.552
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=915
PRIMER_PAIR_3_PENALTY=3.582398
PRIMER_LEFT_3_PENALTY=2.030276
PRIMER_RIGHT_3_PENALTY=1.552122
PRIMER_LEFT_3_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_3_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_3=879,22
PRIMER_RIGHT_3=1790,21
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=60.552
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=912
PRIMER_PAIR_4_PENALTY=3.831224
PRIMER_LEFT_4_PENALTY=2.522443
PRIMER_RIGHT_4_PENALTY=1.308781
PRIMER_LEFT_4_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_4_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_4=875,22
PRIMER_RIGHT_4=1793,20
PRIMER_LEFT_4_TM=59.478
PRIMER_RIGHT_4_TM=58.691
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=919
PRIMER_PAIR_5_PENALTY=3.858872
PRIMER_LEFT_5_PENALTY=2.550092
PRIMER_RIGHT_5_PENALTY=1.308781
PRIMER_LEFT_5_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_5_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_5=876,22
PRIMER_RIGHT_5=1793,20
PRIMER_LEFT_5_TM=60.550
PRIMER_RIGHT_5_TM=58.691
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=918
PRIMER_PAIR_6_PENALTY=4.074565
PRIMER_LEFT_6_PENALTY=2.522443
PRIMER_RIGHT_6_PENALTY=1.552122
PRIMER_LEFT_6_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_6_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_6=875,22
PRIMER_RIGHT_6=1790,21
PRIMER_LEFT_6_TM=59.478
PRIMER_RIGHT_6_TM=60.552
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=916
PRIMER_PAIR_7_PENALTY=4.094179
PRIMER_LEFT_7_PENALTY=2.785399
PRIMER_RIGHT_7_PENALTY=1.308781
PRIMER_LEFT_7_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_7_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_7=878,21
PRIMER_RIGHT_7=1793,20
PRIMER_LEFT_7_TM=58.215
PRIMER_RIGHT_7_TM=58.691
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=916
PRIMER_PAIR_8_PENALTY=4.102214
PRIMER_LEFT_8_PENALTY=2.550092
PRIMER_RIGHT_8_PENALTY=1.552122
PRIMER_LEFT_8_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_8_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_8=876,22
PRIMER_RIGHT_8=1790,21
PRIMER_LEFT_8_TM=60.550
PRIMER_RIGHT_8_TM=60.552
PRIMER_LEFT_8_GC_PERCENT=45.455
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=915
PRIMER_PAIR_9_PENALTY=4.207842
PRIMER_LEFT_9_PENALTY=2.899062
PRIMER_RIGHT_9_PENALTY=1.308781
PRIMER_LEFT_9_SEQUENCE=TCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_9_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_9=878,22
PRIMER_RIGHT_9=1793,20
PRIMER_LEFT_9_TM=59.101
PRIMER_RIGHT_9_TM=58.691
PRIMER_LEFT_9_GC_PERCENT=45.455
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=916
PRIMER_PAIR_10_PENALTY=4.207842
PRIMER_LEFT_10_PENALTY=2.899062
PRIMER_RIGHT_10_PENALTY=1.308781
PRIMER_LEFT_10_SEQUENCE=GTCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_10_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_10=877,22
PRIMER_RIGHT_10=1793,20
PRIMER_LEFT_10_TM=59.101
PRIMER_RIGHT_10_TM=58.691
PRIMER_LEFT_10_GC_PERCENT=45.455
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=917
PRIMER_PAIR_11_PENALTY=4.337521
PRIMER_LEFT_11_PENALTY=2.785399
PRIMER_RIGHT_11_PENALTY=1.552122
PRIMER_LEFT_11_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_11_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_11=878,21
PRIMER_RIGHT_11=1790,21
PRIMER_LEFT_11_TM=58.215
PRIMER_RIGHT_11_TM=60.552
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=913
PRIMER_PAIR_12_PENALTY=4.387949
PRIMER_LEFT_12_PENALTY=3.079168
PRIMER_RIGHT_12_PENALTY=1.308781
PRIMER_LEFT_12_SEQUENCE=GTCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_12_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_12=877,23
PRIMER_RIGHT_12=1793,20
PRIMER_LEFT_12_TM=59.921
PRIMER_RIGHT_12_TM=58.691
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=917
PRIMER_PAIR_13_PENALTY=4.451184
PRIMER_LEFT_13_PENALTY=2.899062
PRIMER_RIGHT_13_PENALTY=1.552122
PRIMER_LEFT_13_SEQUENCE=TCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_13_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_13=878,22
PRIMER_RIGHT_13=1790,21
PRIMER_LEFT_13_TM=59.101
PRIMER_RIGHT_13_TM=60.552
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=913
PRIMER_PAIR_14_PENALTY=4.451184
PRIMER_LEFT_14_PENALTY=2.899062
PRIMER_RIGHT_14_PENALTY=1.552122
PRIMER_LEFT_14_SEQUENCE=GTCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_14_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_14=877,22
PRIMER_RIGHT_14=1790,21
PRIMER_LEFT_14_TM=59.101
PRIMER_RIGHT_14_TM=60.552
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=914
PRIMER_PAIR_15_PENALTY=4.631290
PRIMER_LEFT_15_PENALTY=3.079168
PRIMER_RIGHT_15_PENALTY=1.552122
PRIMER_LEFT_15_SEQUENCE=GTCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_15_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_15=877,23
PRIMER_RIGHT_15=1790,21
PRIMER_LEFT_15_TM=59.921
PRIMER_RIGHT_15_TM=60.552
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=914
PRIMER_PAIR_16_PENALTY=4.653731
PRIMER_LEFT_16_PENALTY=3.344951
PRIMER_RIGHT_16_PENALTY=1.308781
PRIMER_LEFT_16_SEQUENCE=GTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_16_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_16=877,21
PRIMER_RIGHT_16=1793,20
PRIMER_LEFT_16_TM=57.655
PRIMER_RIGHT_16_TM=58.691
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=917
PRIMER_PAIR_17_PENALTY=4.654933
PRIMER_LEFT_17_PENALTY=3.346153
PRIMER_RIGHT_17_PENALTY=1.308781
PRIMER_LEFT_17_SEQUENCE=TCCATCGTACTCAACCTCAA
PRIMER_RIGHT_17_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_17=878,20
PRIMER_RIGHT_17=1793,20
PRIMER_LEFT_17_TM=56.654
PRIMER_RIGHT_17_TM=58.691
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=916
PRIMER_PAIR_18_PENALTY=4.800991
PRIMER_LEFT_18_PENALTY=3.492211
PRIMER_RIGHT_18_PENALTY=1.308781
PRIMER_LEFT_18_SEQUENCE=TATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_18_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_18=874,23
PRIMER_RIGHT_18=1793,20
PRIMER_LEFT_18_TM=59.508
PRIMER_RIGHT_18_TM=58.691
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=920
PRIMER_PAIR_19_PENALTY=4.890326
PRIMER_LEFT_19_PENALTY=1.850020
PRIMER_RIGHT_19_PENALTY=3.040306
PRIMER_LEFT_19_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_19_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_19=876,21
PRIMER_RIGHT_19=1789,21
PRIMER_LEFT_19_TM=59.150
PRIMER_RIGHT_19_TM=57.960
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=914
PRIMER_PAIR_20_PENALTY=4.897073
PRIMER_LEFT_20_PENALTY=3.344951
PRIMER_RIGHT_20_PENALTY=1.552122
PRIMER_LEFT_20_SEQUENCE=GTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_20_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_20=877,21
PRIMER_RIGHT_20=1790,21
PRIMER_LEFT_20_TM=57.655
PRIMER_RIGHT_20_TM=60.552
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=914
PRIMER_PAIR_21_PENALTY=4.898275
PRIMER_LEFT_21_PENALTY=3.346153
PRIMER_RIGHT_21_PENALTY=1.552122
PRIMER_LEFT_21_SEQUENCE=TCCATCGTACTCAACCTCAA
PRIMER_RIGHT_21_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_21=878,20
PRIMER_RIGHT_21=1790,21
PRIMER_LEFT_21_TM=56.654
PRIMER_RIGHT_21_TM=60.552
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=913
PRIMER_PAIR_22_PENALTY=4.997908
PRIMER_LEFT_22_PENALTY=1.850020
PRIMER_RIGHT_22_PENALTY=3.147888
PRIMER_LEFT_22_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_22_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_22=876,21
PRIMER_RIGHT_22=1789,20
PRIMER_LEFT_22_TM=59.150
PRIMER_RIGHT_22_TM=56.852
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=914
PRIMER_PAIR_23_PENALTY=5.044333
PRIMER_LEFT_23_PENALTY=3.492211
PRIMER_RIGHT_23_PENALTY=1.552122
PRIMER_LEFT_23_SEQUENCE=TATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_23_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_23=874,23
PRIMER_RIGHT_23=1790,21
PRIMER_LEFT_23_TM=59.508
PRIMER_RIGHT_23_TM=60.552
PRIMER_LEFT_23_GC_PERCENT=43.478
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=917
PRIMER_PAIR_24_PENALTY=5.070582
PRIMER_LEFT_24_PENALTY=2.030276
PRIMER_RIGHT_24_PENALTY=3.040306
PRIMER_LEFT_24_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_24_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_24=879,22
PRIMER_RIGHT_24=1789,21
PRIMER_LEFT_24_TM=60.030
PRIMER_RIGHT_24_TM=57.960
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=911
PRIMER_PAIR_25_PENALTY=5.096035
PRIMER_LEFT_25_PENALTY=3.787254
PRIMER_RIGHT_25_PENALTY=1.308781
PRIMER_LEFT_25_SEQUENCE=ATGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_25_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_25=875,23
PRIMER_RIGHT_25=1793,20
PRIMER_LEFT_25_TM=60.787
PRIMER_RIGHT_25_TM=58.691
PRIMER_LEFT_25_GC_PERCENT=43.478
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=919
PRIMER_PAIR_26_PENALTY=5.113770
PRIMER_LEFT_26_PENALTY=3.804989
PRIMER_RIGHT_26_PENALTY=1.308781
PRIMER_LEFT_26_SEQUENCE=CCATCGTACTCAACCTCAAA
PRIMER_RIGHT_26_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_26=879,20
PRIMER_RIGHT_26=1793,20
PRIMER_LEFT_26_TM=56.195
PRIMER_RIGHT_26_TM=58.691
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=915
PRIMER_PAIR_27_PENALTY=5.118545
PRIMER_LEFT_27_PENALTY=3.809765
PRIMER_RIGHT_27_PENALTY=1.308781
PRIMER_LEFT_27_SEQUENCE=CCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_27_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_27=879,21
PRIMER_RIGHT_27=1793,20
PRIMER_LEFT_27_TM=57.190
PRIMER_RIGHT_27_TM=58.691
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=915
PRIMER_PAIR_28_PENALTY=5.178165
PRIMER_LEFT_28_PENALTY=2.030276
PRIMER_RIGHT_28_PENALTY=3.147888
PRIMER_LEFT_28_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_28_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_28=879,22
PRIMER_RIGHT_28=1789,20
PRIMER_LEFT_28_TM=60.030
PRIMER_RIGHT_28_TM=56.852
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=911
PRIMER_PAIR_29_PENALTY=5.274765
PRIMER_LEFT_29_PENALTY=3.965985
PRIMER_RIGHT_29_PENALTY=1.308781
PRIMER_LEFT_29_SEQUENCE=GTCCATCGTACTCAACCTCA
PRIMER_RIGHT_29_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_29=877,20
PRIMER_RIGHT_29=1793,20
PRIMER_LEFT_29_TM=56.034
PRIMER_RIGHT_29_TM=58.691
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=917
PRIMER_PAIR_30_PENALTY=5.274765
PRIMER_LEFT_30_PENALTY=3.965985
PRIMER_RIGHT_30_PENALTY=1.308781
PRIMER_LEFT_30_SEQUENCE=TGTCCATCGTACTCAACCTC
PRIMER_RIGHT_30_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_30=876,20
PRIMER_RIGHT_30=1793,20
PRIMER_LEFT_30_TM=56.034
PRIMER_RIGHT_30_TM=58.691
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=918
PRIMER_PAIR_31_PENALTY=5.281379
PRIMER_LEFT_31_PENALTY=1.850020
PRIMER_RIGHT_31_PENALTY=3.431360
PRIMER_LEFT_31_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_31_SEQUENCE=GGACCCTTTGACCAATCAATCT
PRIMER_LEFT_31=876,21
PRIMER_RIGHT_31=1790,22
PRIMER_LEFT_31_TM=59.150
PRIMER_RIGHT_31_TM=61.431
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=915
PRIMER_PAIR_32_PENALTY=5.339376
PRIMER_LEFT_32_PENALTY=3.787254
PRIMER_RIGHT_32_PENALTY=1.552122
PRIMER_LEFT_32_SEQUENCE=ATGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_32_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_32=875,23
PRIMER_RIGHT_32=1790,21
PRIMER_LEFT_32_TM=60.787
PRIMER_RIGHT_32_TM=60.552
PRIMER_LEFT_32_GC_PERCENT=43.478
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=916
PRIMER_PAIR_33_PENALTY=5.357111
PRIMER_LEFT_33_PENALTY=3.804989
PRIMER_RIGHT_33_PENALTY=1.552122
PRIMER_LEFT_33_SEQUENCE=CCATCGTACTCAACCTCAAA
PRIMER_RIGHT_33_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_33=879,20
PRIMER_RIGHT_33=1790,21
PRIMER_LEFT_33_TM=56.195
PRIMER_RIGHT_33_TM=60.552
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=912
PRIMER_PAIR_34_PENALTY=5.361887
PRIMER_LEFT_34_PENALTY=3.809765
PRIMER_RIGHT_34_PENALTY=1.552122
PRIMER_LEFT_34_SEQUENCE=CCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_34_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_34=879,21
PRIMER_RIGHT_34=1790,21
PRIMER_LEFT_34_TM=57.190
PRIMER_RIGHT_34_TM=60.552
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=912
PRIMER_PAIR_35_PENALTY=5.461636
PRIMER_LEFT_35_PENALTY=2.030276
PRIMER_RIGHT_35_PENALTY=3.431360
PRIMER_LEFT_35_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_35_SEQUENCE=GGACCCTTTGACCAATCAATCT
PRIMER_LEFT_35=879,22
PRIMER_RIGHT_35=1790,22
PRIMER_LEFT_35_TM=60.030
PRIMER_RIGHT_35_TM=61.431
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=6.6000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=912
PRIMER_PAIR_36_PENALTY=5.518107
PRIMER_LEFT_36_PENALTY=3.965985
PRIMER_RIGHT_36_PENALTY=1.552122
PRIMER_LEFT_36_SEQUENCE=GTCCATCGTACTCAACCTCA
PRIMER_RIGHT_36_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_36=877,20
PRIMER_RIGHT_36=1790,21
PRIMER_LEFT_36_TM=56.034
PRIMER_RIGHT_36_TM=60.552
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=914
PRIMER_PAIR_37_PENALTY=5.518107
PRIMER_LEFT_37_PENALTY=3.965985
PRIMER_RIGHT_37_PENALTY=1.552122
PRIMER_LEFT_37_SEQUENCE=TGTCCATCGTACTCAACCTC
PRIMER_RIGHT_37_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_37=876,20
PRIMER_RIGHT_37=1790,21
PRIMER_LEFT_37_TM=56.034
PRIMER_RIGHT_37_TM=60.552
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=915
PRIMER_PAIR_38_PENALTY=5.519666
PRIMER_LEFT_38_PENALTY=4.210886
PRIMER_RIGHT_38_PENALTY=1.308781
PRIMER_LEFT_38_SEQUENCE=ATATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_38_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_38=873,24
PRIMER_RIGHT_38=1793,20
PRIMER_LEFT_38_TM=59.789
PRIMER_RIGHT_38_TM=58.691
PRIMER_LEFT_38_GC_PERCENT=41.667
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=921
PRIMER_PAIR_39_PENALTY=5.562749
PRIMER_LEFT_39_PENALTY=2.522443
PRIMER_RIGHT_39_PENALTY=3.040306
PRIMER_LEFT_39_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_39_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_39=875,22
PRIMER_RIGHT_39=1789,21
PRIMER_LEFT_39_TM=59.478
PRIMER_RIGHT_39_TM=57.960
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=915
PRIMER_PAIR_40_PENALTY=5.590398
PRIMER_LEFT_40_PENALTY=2.550092
PRIMER_RIGHT_40_PENALTY=3.040306
PRIMER_LEFT_40_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_40_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_40=876,22
PRIMER_RIGHT_40=1789,21
PRIMER_LEFT_40_TM=60.550
PRIMER_RIGHT_40_TM=57.960
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=914
PRIMER_PAIR_41_PENALTY=5.668280
PRIMER_LEFT_41_PENALTY=4.359500
PRIMER_RIGHT_41_PENALTY=1.308781
PRIMER_LEFT_41_SEQUENCE=CATCGTACTCAACCTCAAACATC
PRIMER_RIGHT_41_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_41=880,23
PRIMER_RIGHT_41=1793,20
PRIMER_LEFT_41_TM=58.641
PRIMER_RIGHT_41_TM=58.691
PRIMER_LEFT_41_GC_PERCENT=43.478
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.3000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=914
PRIMER_PAIR_42_PENALTY=5.670331
PRIMER_LEFT_42_PENALTY=2.522443
PRIMER_RIGHT_42_PENALTY=3.147888
PRIMER_LEFT_42_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_42_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_42=875,22
PRIMER_RIGHT_42=1789,20
PRIMER_LEFT_42_TM=59.478
PRIMER_RIGHT_42_TM=56.852
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=915
PRIMER_PAIR_43_PENALTY=5.697980
PRIMER_LEFT_43_PENALTY=2.550092
PRIMER_RIGHT_43_PENALTY=3.147888
PRIMER_LEFT_43_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_43_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_43=876,22
PRIMER_RIGHT_43=1789,20
PRIMER_LEFT_43_TM=60.550
PRIMER_RIGHT_43_TM=56.852
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=914
PRIMER_PAIR_44_PENALTY=5.759885
PRIMER_LEFT_44_PENALTY=4.451104
PRIMER_RIGHT_44_PENALTY=1.308781
PRIMER_LEFT_44_SEQUENCE=ATGTCCATCGTACTCAACCTC
PRIMER_RIGHT_44_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_44=875,21
PRIMER_RIGHT_44=1793,20
PRIMER_LEFT_44_TM=56.549
PRIMER_RIGHT_44_TM=58.691
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=919
PRIMER_PAIR_45_PENALTY=5.763008
PRIMER_LEFT_45_PENALTY=4.210886
PRIMER_RIGHT_45_PENALTY=1.552122
PRIMER_LEFT_45_SEQUENCE=ATATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_45_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_45=873,24
PRIMER_RIGHT_45=1790,21
PRIMER_LEFT_45_TM=59.789
PRIMER_RIGHT_45_TM=60.552
PRIMER_LEFT_45_GC_PERCENT=41.667
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=918
PRIMER_PAIR_46_PENALTY=5.825704
PRIMER_LEFT_46_PENALTY=2.785399
PRIMER_RIGHT_46_PENALTY=3.040306
PRIMER_LEFT_46_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_46_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_46=878,21
PRIMER_RIGHT_46=1789,21
PRIMER_LEFT_46_TM=58.215
PRIMER_RIGHT_46_TM=57.960
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=6.6000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=912
PRIMER_PAIR_47_PENALTY=5.858729
PRIMER_LEFT_47_PENALTY=1.850020
PRIMER_RIGHT_47_PENALTY=4.008710
PRIMER_LEFT_47_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_47_SEQUENCE=ACCCTTTGACCAATCAATCT
PRIMER_LEFT_47=876,21
PRIMER_RIGHT_47=1788,20
PRIMER_LEFT_47_TM=59.150
PRIMER_RIGHT_47_TM=55.991
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=913
PRIMER_PAIR_48_PENALTY=5.911622
PRIMER_LEFT_48_PENALTY=4.359500
PRIMER_RIGHT_48_PENALTY=1.552122
PRIMER_LEFT_48_SEQUENCE=CATCGTACTCAACCTCAAACATC
PRIMER_RIGHT_48_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_48=880,23
PRIMER_RIGHT_48=1790,21
PRIMER_LEFT_48_TM=58.641
PRIMER_RIGHT_48_TM=60.552
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.3000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=911
PRIMER_PAIR_49_PENALTY=5.933287
PRIMER_LEFT_49_PENALTY=2.785399
PRIMER_RIGHT_49_PENALTY=3.147888
PRIMER_LEFT_49_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_49_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_49=878,21
PRIMER_RIGHT_49=1789,20
PRIMER_LEFT_49_TM=58.215
PRIMER_RIGHT_49_TM=56.852
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=912
=
SEQUENCE_ID=MAGIC.446_5_15111967_15112389_mt-0_rsch-4_19659_51203_TRUE
SEQUENCE_TARGET=950,522
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGATCNTGTCCTTTCTNGTTANTTATTNCCTACAAAAACAANCAAATTTNACCAATAATTGTTCAGNGTTAGTTATTTAACNTTTTAGAGAAGATTNAGCATTAGAGCACAAAATAAGAGCCATCTATCGCTACTTTCTGTACCAAAAGGTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAGAAAGTTAGTTGGAAACGATNGAGGAATTGTNGATGTGGATGNGCGGACCATCACCNNNNNNCCGTTCATTGGAANNNATTGAGGAAAATCCATTGCTGATAATAAATTCTCAACAGTTNTATTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATTTAATTTGGAAGGNTTTTGCAGAAGTTNTTCGAATATGATATCTCATTCTCGCCAAGTCCAAANAGTTAAACNTACTANNNTTATGACTTTTGGCGTTAAACAANTGTAANTTTCTTCGATGTAANATGAATTCAGAATCACGTGTTTTGNNCCCTACCATTTCCATAATTTTGTTTCCTNCGTAAANATGTTTANNNNNNNNNAAAAANNNNNNNAANCTTTGTCCCCGCNNNNNNNNNNNNNNNNNNNNNNNATTCNAAATTCATGGTATGACTTTTGGCNNNNNNNGTTACGCTACTNTAATTAAGAATCACGNGTTTTGGTCCTACCATTCTATAATTNTGTTTCCTACGTNCANNNNNNNNNTACTGTTNAGCCAATAANTTNNAAANATNTTTGTCCCCGCATATCAATTATATAGCTGATTNCAACCTATTTTTAAATCTATTTNTGGTATTTTTNATAAAGAGNTNNCTTTTTCTTACAACGATTGTTCCNGACTNTNNNNNTTTTTTTGGANGGCAAGTATNGCTCNNNNNNNNNNNNNNNAATGTTTTNTCNAAATTNNNNNNNNNNNNNNNNNNATTGGCATTTNTAACAATGATTTNATNNNAAAAAANNNNNNNNNNNANACAAAACNACGGTNTTTTGTANTNNNNNNNATGTGGCATCCTAGTCACTNNNNNGNNNNNGTTTTGAATTATTATCTGTATGACATGCTGATCACCTTGGTGATGACAGCCAAGAAATGAGACAGGACCAGTCAGTTATGTTACGTTTGTAACCTGGCTGGCCGAGACGTCTAAGAATCTGATGCCAAATCTGAATATTATAATCACAGTAAATCNAAAGATGATCGCGTGTCTCCGATTGCTGGTTGCAAAGGAAACAAGATATGNCTGTAGTAATACCCATGCGATTCTTTATTCTTCTATGTTCGTGGCTTATAAATTGAAAATAGAAACATTTATATCCATGTTACGTAAAATATCAAACTAAAAAAGTATGCTAATAAGTTATTTGGGGCTGATTTTTCAATTGGATGGTAATTACAATTGAAATAATGAAGTAGATGAATCAATTGGAGCATGACATGACCGCGCACATGGAATGATATTTTTTTT
PRIMER_LEFT_NUM_RETURNED=8
PRIMER_RIGHT_NUM_RETURNED=8
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=8
PRIMER_PAIR_0_PENALTY=0.952696
PRIMER_LEFT_0_PENALTY=0.799535
PRIMER_RIGHT_0_PENALTY=0.153161
PRIMER_LEFT_0_SEQUENCE=TGAGGAAAATCCATTGCTGA
PRIMER_RIGHT_0_SEQUENCE=AATTGATATGCGGGGACAAA
PRIMER_LEFT_0=911,20
PRIMER_RIGHT_0=1712,20
PRIMER_LEFT_0_TM=59.200
PRIMER_RIGHT_0_TM=60.153
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=802
PRIMER_PAIR_1_PENALTY=1.457995
PRIMER_LEFT_1_PENALTY=1.304835
PRIMER_RIGHT_1_PENALTY=0.153161
PRIMER_LEFT_1_SEQUENCE=TTGAGGAAAATCCATTGCTG
PRIMER_RIGHT_1_SEQUENCE=AATTGATATGCGGGGACAAA
PRIMER_LEFT_1=910,20
PRIMER_RIGHT_1=1712,20
PRIMER_LEFT_1_TM=58.695
PRIMER_RIGHT_1_TM=60.153
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=803
PRIMER_PAIR_2_PENALTY=1.959780
PRIMER_LEFT_2_PENALTY=0.799535
PRIMER_RIGHT_2_PENALTY=1.160245
PRIMER_LEFT_2_SEQUENCE=TGAGGAAAATCCATTGCTGA
PRIMER_RIGHT_2_SEQUENCE=TAATTGATATGCGGGGACAA
PRIMER_LEFT_2=911,20
PRIMER_RIGHT_2=1713,20
PRIMER_LEFT_2_TM=59.200
PRIMER_RIGHT_2_TM=58.840
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=803
PRIMER_PAIR_3_PENALTY=2.465080
PRIMER_LEFT_3_PENALTY=1.304835
PRIMER_RIGHT_3_PENALTY=1.160245
PRIMER_LEFT_3_SEQUENCE=TTGAGGAAAATCCATTGCTG
PRIMER_RIGHT_3_SEQUENCE=TAATTGATATGCGGGGACAA
PRIMER_LEFT_3=910,20
PRIMER_RIGHT_3=1713,20
PRIMER_LEFT_3_TM=58.695
PRIMER_RIGHT_3_TM=58.840
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=804
PRIMER_PAIR_4_PENALTY=3.011818
PRIMER_LEFT_4_PENALTY=0.799535
PRIMER_RIGHT_4_PENALTY=2.212283
PRIMER_LEFT_4_SEQUENCE=TGAGGAAAATCCATTGCTGA
PRIMER_RIGHT_4_SEQUENCE=ATAATTGATATGCGGGGACA
PRIMER_LEFT_4=911,20
PRIMER_RIGHT_4=1714,20
PRIMER_LEFT_4_TM=59.200
PRIMER_RIGHT_4_TM=57.788
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=804
PRIMER_PAIR_5_PENALTY=3.517117
PRIMER_LEFT_5_PENALTY=1.304835
PRIMER_RIGHT_5_PENALTY=2.212283
PRIMER_LEFT_5_SEQUENCE=TTGAGGAAAATCCATTGCTG
PRIMER_RIGHT_5_SEQUENCE=ATAATTGATATGCGGGGACA
PRIMER_LEFT_5=910,20
PRIMER_RIGHT_5=1714,20
PRIMER_LEFT_5_TM=58.695
PRIMER_RIGHT_5_TM=57.788
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=805
PRIMER_PAIR_6_PENALTY=5.727213
PRIMER_LEFT_6_PENALTY=0.799535
PRIMER_RIGHT_6_PENALTY=4.927678
PRIMER_LEFT_6_SEQUENCE=TGAGGAAAATCCATTGCTGA
PRIMER_RIGHT_6_SEQUENCE=TATAATTGATATGCGGGGAC
PRIMER_LEFT_6=911,20
PRIMER_RIGHT_6=1715,20
PRIMER_LEFT_6_TM=59.200
PRIMER_RIGHT_6_TM=55.072
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=805
PRIMER_PAIR_7_PENALTY=6.232513
PRIMER_LEFT_7_PENALTY=1.304835
PRIMER_RIGHT_7_PENALTY=4.927678
PRIMER_LEFT_7_SEQUENCE=TTGAGGAAAATCCATTGCTG
PRIMER_RIGHT_7_SEQUENCE=TATAATTGATATGCGGGGAC
PRIMER_LEFT_7=910,20
PRIMER_RIGHT_7=1715,20
PRIMER_LEFT_7_TM=58.695
PRIMER_RIGHT_7_TM=55.072
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.1000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=806
=
SEQUENCE_ID=MAGIC.433_4_4702389_4702667_rsch-4_oy-0_58699_22139_TRUE
SEQUENCE_TARGET=950,378
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14529457_14529541_ler-0_oy-0_9620_11428_TRUE
SEQUENCE_TARGET=950,184
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_5643426_5643642_oy-0_wu-0_5372556_21751_TRUE
SEQUENCE_TARGET=950,316
SEQUENCE_TEMPLATE=ATTTCCGCGAAATCGAGAGAGAGTNAGAGAATTTTCTGGTTGGGNGAGAAATTGAGATTGGCAGAGAAAACAAGATGTTTTTTGCTATGAGTGTAAAGATGAGAGCAAATAGGGTATTTATATCTCGTTTCCAAGNGAAGCCATTGAATCCCTAAGATTGCAAGTGTGACCGTTGTTGGAGCGGAGCTTTTNAATTTTGAATTTTTNGGGAAATTAATATTTTAATTAGTTTCTCTTACACTCTCCACCTTGTCATGTATCTGCCNAAAAAAGGAAGAAATATTCCGANAAAGGACACCGGAATAAGAAAATTACAGTATTTCAAGAGCATTTTAGGAATTTTTAATTCAAATCATAGGATTTTAATAGTNNTTTTATNNAGTTATNGGCCATATTTGAGTAAANATAAAAGTATNGTTATGGGCCATNTAAACAATACAGCCCAGANAAGGTGTTANGGNCCATNCNTTGTGTANGGAGTAATCACATTGCNATATAACTTGTTTAGNNNTATGATCNAAANTTCANNNNNNNNTTTATCNTACCTTTCTCTTTNTTAGTTTAGTTAATATATGAACCAGAAGTAANNTTTCAAACTNNAACAAGTGATACATTCANACNTCATAACACCANAANTTAGTTCGTTTCGAAACATNGCATAACANTTTTACTTCAAATTTNATTTGNAAAACACAAATNAAAGGANGAGTTTGTTTGCTACGAATGNTGAGTCTTGTCCANATCNTCGAGACTTGTTGGACGGAACCANGCTTTCTCCTCCATCACTGAGTCCTCCTGACCGNCGCGAAATTCGTCCNTTGNGTNNGTCGANGAGGAGGAAGGTCCAAAGACACCGGTTTGNGGATGAGGNGACCAATATTNGTCAGAGTCAGGCTTTATCATNTCATCAGGCACTTNNCTNGNTTGCAATTCTTCCTCGACGTTCTTATCATACGCAGANGTGTGANCATTCCTGTTCAAGAAGANAACAAANTTGTTTNTAGCAATAATATTGGTGAATGAGTGAATCTAAGTGTTTGAGATTAAAATTTGNAANTCGTTAGGTGAGATATTGATCGACTGTACTAACCGTATGAATAAAACAAGAAAAATGGTTAGAAGATAAGATAACCCCACAAGAACAACTACATCTTTTATCACTTACCANCCCTAGCAGGTTTATCTTCAATTCCGATANTCNAAACATCCNATNTNTNNNAAAAGAGAATGGTATCNGAAATCNATGGCTATTGANATNTCTATCCACTAACATATTCTNACANGNTGTCGGTCCATTNATTTATATTTGATTTTATAAATAATNTNTACATGAACACNAAACTCTAGATTTTAGTTTTACTAATTAAATAGACATCTGTATTCGTCTANATTCAGAATGTGAAGTGAAGAANGTACANCTTGTTTACAAGGTCAAGATGATTACTTTTGAAACAAAAATATACATGGACCATGCCACCATGGCCNCTTCAACCAAGAAGATTNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_15521440_15521948_oy-0_rsch-4_99954_130684_TRUE
SEQUENCE_TARGET=950,608
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTGCCCGTTTTCATTTTTGAAGGNGAGAGAGAGACTGTAAGGCTGNCNGACAGATAACTNGTCNGNAACCNAGAAAACTTGCCNNAATTCAGTAAATCTTGCNCGTGNTNGAGGAAACTNTGTCCNNGNCTNGAGAATCGNCGCCTGTGGTCNAGAGAACTTGACCGTGAGTAAGGGATNGTCGACCGTNATNGAGGAATTTTGCCCGAGNCCCANAAANCTTCCNAATTACCGAATNTGTGAGTTGTGGTCGAATTGTGTGTTTGTTTGCATGTTTTCTAGAGCTAGTCTTTAGATCATATGNATGCATAGTCACTTAGACTTAACTAATCGGNATTCGGNTTAAGTTAGAACTTGAATCTTAAAATGATGNNNNCTTGATGAACTTGATANNTAATATCATTAAGGAATGNNATGGACTGGTNCNTGACTAGGCTAAGTGAATANGGACTTNACTAANTTTACTTGATGAATNGAGATTTGATTCNANGGATTACTTGCCTNGTTAG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_14473124_14473133_sf-2_ws-0_17360_11429_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NACTTGGTATATTATAATAAAATTATNTGTGNTTTNNNNGAAGAGAATTATGGTCNTTTTACGAATCTTTGTAATGTTTGGTATCTACGATGGTGCATACCGCGAGTTAGCCAAATGTTATGTATTTANTNGGAAACGCGTTTCCATGACGTTTTTTGTGTTTCCAACCTCAGAGCGAATTTCCNTGCNNCCNTNNCNATCGTCNTTGGNATCTGCTCCACCGNCNATTNANATCCCATGCATTCTTGNTGGGAATCANTGTCCAAGCAATNNNNNNNNNNNNTNTCTTGCATCATTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTNTNNTTNTATNTTATTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_9016724_9016753_wil-2_sf-2_882_596292_TRUE
SEQUENCE_TARGET=950,129
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATANTGAATATAGNTACNAAATAATATAATGATANATTATAATATGACAAATCACAATTTATATNTTTATGTTGTTCGATTTGTGACTACNTATTTGTGTTTGAAAAGTAGTATGATATGTTTTATTNACTACAAGAAAGACAACATTNAATTCNTTTTTGTTTGAATAACGTAACTNAATCGACCACTAAACTATCATACACGTNCTTAAAAAATGATGTTAAGTTTAATGTGATCCCCAAGTGTTGNAATTGGGTTGAGTAATAAATAGAAGTGGACAAATGGTTANTNANTTATTTTCCTAGCTAAATTCAAGTATTTTTCAAAATTATTTTGTTTCAAANTAAAACTTNAAAGTCATAACAAATTCTANNGAGTTTTAAAAATGACCAACAAATTGAAAGTGTGGTTGTTGCAAATGNAACTTNGTGGAGATACGGTCAGTGACAGCCNNACACGACTCCATCGGTTCGATCAAATACCTCACGTAACCCGCCGATTCACTCGATCGTCTTTATTATTCTTTTCNATTTTCAATCATATTGAGCCTGATTGATAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_13615879_13615966_zu-0_no-0_162239_40949_TRUE
SEQUENCE_TARGET=950,187
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_22150038_22150363_can-0_hi-0_397093_530047_TRUE
SEQUENCE_TARGET=950,425
SEQUENCE_TEMPLATE=GCTCAGGTTCGTCGTCGGANTCATTGGCATACGAATTGCAGATGAGATCCATCGGAAAGTTGAAGTCGANNCACCCGAAGCAGTGACCGGTGATCGGAAAAAAANTGAATTGGCCTTCGATNNGATAGAGAAGGCTAAATCTTTAGTGGGCTTTAATTGGGCNAGAAGAATGCTCCAGGCCCACGTGTATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACGCGTGTAAAACTGCATGAGCCACACGTGGGCCAAGCTAACCGGTTACCTGCTGCGTCTTAAACAGTAACACACAGAACAAACGACCGCTACGAACATAAGTGACAACTCATCTCCAGGTAACCGGTACAAATGGGAACCACAGAAAANNNACGTGGCANAAATCTATAAGCTACCGTCAAGTGAAACCGGTCCCCTGATTGGTTAACGTAATCATTTTCTCAATTCTGGACCCTACTGTAGTACTATGTATCGCGTGACTCTCTCANAGTGGCATATTTGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4325887_4326165_wu-0_tsu-0_197919_119823_TRUE
SEQUENCE_TARGET=950,378
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9193779_9194145_col-0_po-0_15161_124098_TRUE
SEQUENCE_TARGET=950,466
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAAGGCAGGTGGTAAAACTAANTAAGATTCCTCTCTTTTTTNCCATCGTCTCAACTACTTCNCCATTGAGTTTATACTAATCATGCAGTTNTTGTAAGAAAAANAAAAAAAAATACTAATCATNCAGTTTATATTCATCCACAAGTCATTATCACTATGATCAGCCGACGAATTTAATAGATAGTTTGACTATTGAGTCACTGACTAAAAAGGTTTGTATATCAATCTTATATCATTTGGAATNCATTGNTCACAACCTACAATTTGAATGAATCGAAGTATNTCACNTAAAGGTGCCGAATGACTTTAGGATAAGAATATAAGTCNAAGTCTAAGTANATCCGTATGAATGAAAAATACAAAGAAGCTGAGATTTTATATATAGACTAATATCATAGCTAACGGTCAAAAGACTACAATAAGGAAATAAAGACTCGAATAGGAAAGAATCCCAAGTGATGGCCATTGGCGCCTTTGATGATATCTTGAAATTTGAGAAGAAATATAAGTTTNAAAACNGTTTGCTTCAAAGANCTTCAAAATAGNNGTAANTTCCCAGAGAANCCTAGGGCTAATAAGTTTCCTCAACTTGAACAGACTTAAGAGAGATTGGAAAATTAAGGAGAAATCTTGAAGATGTGACAATTCTGNAACTATGACTGATNACCATTANTNTNATCGCNGTTTAGTTTTANTTGTTGGTTTTNACAGGTTAAGTTGG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_6862042_6862345_ler-0_po-0_2403_27750_TRUE
SEQUENCE_TARGET=950,403
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAAAAACACNANAACAGTGTAAATTGTTNNACTNTANATTTATTTTAGTGGCTGAAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAATCACACTGCCGTTANATTATGTGNCANAGTAAAATANAATANGTNAANNNNNNNNNTATNNATNNTAAATCTTNNNAAAANNCTTAAANCACAATGAAATGNCACATTANCAACCNAAAAAAAATGGTTCCTNNTTCCTACTCTTTTAATANACTTTGATTTTTATACGTGTTTTNGTAGTTCATTCGANCTATTCTAGGTAGATTAACCAACCATATGTAAATGATCTAGTCATCCTCAAAATTAGTCAAACGCTAGTNCAATATAAATTTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAATAGACCGCCTTNACAGANGCCAAATTCTATTTTTCAGTACATNAAGACAATATTGANATTATTGTTTTGACTTTTAGACNAGGAATTACNATGCNTNTGNNNNCTACGATATTACNAATATAATTTTCTGGTCAAATGGNNAAAAANNNTATTAAAAANGGAGAGAGAGATGNTTNATCGATAAAAGTAAAACTAGAGACAAAGGTCGTTGTTATTCATCGANCACCCACATGTAACTAANTANNANGAGTTNATTTGTTACTNTGTCAACTAAAACCGTTGACACTATCCAAAAATAATTTTGAATCTCCTTGTTATGACTTTTGCTTCAAATTAAGCAAATCAGTGGAAACTTAACATAATGATTTTCGTTGAGANAACATCAGTCTNATAAAAAAAAAGTTCTTTATATTCAGTTAATNTGAAAAAACTGAAGAAACAAAAATTACAGNAACAAATAAAAATANANAAAAAAGATATATANATGAACAAAGATAAGTATTAGTAGTACACTAATGTTTTGTTTGAGAAGAGGAAAAANACAGAAATTAGTCTNTAAGACAAAAGTCGTTGAGATTGTGTTGGTCAAAGTGAAGGCTTCAAAATCATGGTTTAACAGTTTTTGATATTGACTAGAAAAATCTTTGTGGAATGTTACTGGTCTTTAAGGCTGCCAAAAANNTCATTTATTTCNTTCACGACATTAAGTGGTCCAATGTCTGCCACTATCAACATTACTGATTCTAGCTTTCANCAATAAGAAAATTTTGAACCATATAATATTCGCNTTAATTCAAAAGCATTCTAAAAATGTAGAGACAGATGGAATTCTACAATTCTATTTAATACAAAAGCTTACACTTTTCATTTTTAGCTTATTCTAAGCTGTCAAGTGCTTAGCATTAATTNTATTGTTANTATGAATACGTGTTTTGAGTGCATTATTGNAAAGAAGGTGGAGATCTCAACCGTCTNCAATGAGTGATTTAGGAGTNTATCTTAAATAATTTGCATNNNNNNNNNNNNNNNNNTTTTAACATAAAGAATCCTATAATTAGGTTTTAAAATCAAAAGCAAGAACATTTCTAATATTAATGCCTATTAAAAGGGGCACATTTCAACTGTTCTGACCTCTAATTCTTGTTTATGCCGACATGATGTTTGCAGTAGNCAGTAATATCAGTATTTTTCAATAAANTCTAGCTCTTTAATACANGATCGGTAATCTAGGAAAAGCCCCAATTCTTACATGGATANTTNTTTTTGTCATCGTGACGATATNAAGANNACCTAATTGGCCTGNCAAAATTTACACAAATTTTNTTCTTNCATAAACTAAAANAGNTGACCAAATAATGACAATTTTATGTGTAATATTAGAAAATTGTTATTAAAATTTTCTTAGCAAATATTGANNTNNNTTTTCTCNACGATATTATATATATGAAATNTTATGNATAAGTTATTACAATNGCATGTTCTATAATTTGAACGGATAACNAAAAGNAAAATTTAGATTTNATTTATAANATAAAAAANAATGAAAATTCNAAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_17026872_17026986_ws-0_oy-0_2936_787_TRUE
SEQUENCE_TARGET=950,214
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5931868_5931932_oy-0_mt-0_17990_1313_TRUE
SEQUENCE_TARGET=950,164
SEQUENCE_TEMPLATE=NGGGTACTTNNCNGCAANNTANTCAGAGTATTCCAAGTANANNAGATTTGGTGTATCAAATGAAANANTATCCGGATTCTGATCNTAGNCTATACAACATAGCTTCAATCTNTTAAAGGTTTTGGAAGACACAGAGNGGTTCNTAAANTCAAAGTAGACATNATNAGAACCAATTCCTCTAAGANAAGACAACCNGATAGAATCTTGTCAANGTAATCANCNGCTTTTCCTANANNAANAGTATCAAGNTNAAGAGTCTTAAGCTTNGGAAGNNAANCATCTTNCACATCAATGNNAGTAACNTTTTGAGANTCTATCCTCAGACTAACAAGTGACTCACTCANAAAGACCTTAGAAGGAAGCTCANCACNATCNACACTAACGTGTAAATCANNTNCCAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGAGAACTTGNTTACNATNNANTTCTCTTGCAAACNCAAAACTCTNTTCANGAAATNNATAAANATTNTATTACNATCTTCACGATGATGTAGATTAACNAAGCCATCNAAATCAANATTTGGTCTGAAAGNAAAGAGNTAANGCCACTTTTTNGAGAGAAGTGAAGTCANAGCAGAATCTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGNTTGTTGATNNTGTCTTNGGAACTNAAGNNTATCTTTTTCGACTTCATAGTGACANATCTCAACANNAGTTNNAANCTAAAAGGGAAGATTTTTNAGAGAANNTCANAAGNTTAANCCNAAAAGANNAGANCCCANAAAAGNAAANNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNAAGAANTGAGGGANCNNNNNNNNTGNNAATTTTGAANAGCNNGATTCAGTNGNTNANNGTGNGAGANANGAAGTGAANGAAGAGCANANNAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNACGTCGNGTGNTNTTTTTTTTNNTNNCNNNTTAATTNNNCTGTNTTACAAAAGCAATTTNTAATTTTTTGTTGTNNGATAAAATTTAAGAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTAGTTTTAAAGTGGCNTTTTTTTNGTGTGGTTCTAGNGTNTNTTNAGTNATTGGNTNTNCTATACANNANATCNTTANNNNNNNNNNNNNNNNNNNNNNCNATANAATGAAANATATACNNTANCNTTTNAAAGAATGAAAAAATAAATTAANAGACAGTTTCGNAGAAGANTTTTTTNNNNNNNNNNNAANTANATGANANATATGAAGAGATTAGTGNCGTGAAGGCNACTCATGGTTTGCNTCTTCTTCTTTAGTTTGTTGCTTTGTTTCAGCATTNGCCACTTTTTTGTCTATCAAAGGCTTTATCATTGCCTCCAAGTCATCTATATCTTGTGTGACTTTTTCCACCTGCNATTTNTNNGTTTTGACTTAGTCAATCACANTCANANNTNTATATATGTTTGNNNATTATTNGGTTCCAAAANATCCCCCAAATGATAAACCAATAAAAGNNACCCAAANANATANATCATATNTTCAAAATGTAATCNTTTACNTTAGCCTTTGTTAGTGTTNNNNNNNNNNNAGATTTTTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNATNAAGTGTNGTGGTNTACCTTGTGAAGGAAATCTTGGGTTANATGTGCTTCATGGGCAGCAACCTCTGAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14381107_14381185_tsu-0_can-0_14412_6858_TRUE
SEQUENCE_TARGET=950,178
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_9445657_9445689_kn-0_ler-0_388509_404552_TRUE
SEQUENCE_TARGET=950,132
SEQUENCE_TEMPLATE=GTTNAACACGTAATATATGACTCAATGTTGGAGGATAATTTNTTGNACCAATATTCTGTATGATGGAAANNGAGNTTAAAAATNAAATTAATCACAACTTCCACAAGTNGAAAATTTAAAAATTTGTTTAGTGACCTAATCATGTAACTATACTTTTAGATNAATACTCAAGTCTATTTNTAANATTATATGTTGTTAGAAATTTTCAAAAANNATATATATGATGANAAGNTTGAAGATAAAGTGGTGAAAGTTTAACACACATGGTTGGTTGGGGAGTTATTGTAGCTACATGCTTTCCACATTTAATTTCTTTTTTCGAACTTTTATTTAATAATATATACAGAATATATACCCTGAAAANAACTAATTAATTGATTAACAATATTATTATTTAGATATAGAATTTTNTAGGAGCTAGAAAATAAAAACAAATGANTTGTTGATTCTAGGATTCGTTGAAAGGCCAATTTGCTTTCCAACNATTTNACGATCAAGTGAAAGGACCAATAACTCTTGGAATATGGCACCTTTTATATTATGAACATTTTANTTTNATTTGGACAAATATATTAAAAGCTAATTGCATNGAAATTCTCTCNNCNGTGCGTTTTCCNTTTNCNAAACATTTCANAAAACNGAAACTNNTGNAAATTNGATGNAAANTCACNAAAACNCGTTTCNTAAAATTTNATNTTTGGAAACACGATGAAANTTTTTATNTTTTNGAAACGCAAAAANTCTTNTTNTTTTACTTTGGAAACTTAATAATAATAAAAGTTTGGAAAGATAAANATTTAACTAATTTTGGGAGANTCNAGTTTTAAGATCTTTCTCTCGACGAATCAAATNTCATGTGNTTNATATNATTTTTNATNGAACTAATGAAAATNNTGGTCTAAATATTTGATATTANGCTTTTTAATCTTTATTTTTAAGAATTTTACTTTANNTATTTGATATTTATGTTTTAGATTTTTGTTGTTTAANNNNNNTATATNTATATATATANATNNNNNTANGTTTCCAAAANNTTTGCGTTCNTTAATTTTANAAAAAAANTNTTTTCGGTTTTNGAAACGTTTNGTTTCCGTGTTTCNGTTTCTGTTGNTATNCAACATANATTAAAANTACGAGGTAGATANAAAAAAATAGGAATANGTTATTGTTGGTTTATACTTTATANTNGTATTACGCCCATNATATGACAGTATATCGAACCGGNAATTGGAAAGTTAATTCCCAAAGAATANGATTATACTAAGATGAAAAGATGATTGTAGAGAGANAAAAAAAGAGCTTTGATGAGCTTTGTGCCNATTGACACAAAGAGAACTTTAGATNGANAAAANGAACGTTTTATAGAGAGAAAGCTTTGAAAGAACAAAAGGAAAAGCAATTGAACGTTGATTTGTGGTTGATTTCCAATCTCTACATTGATTACCAGTCAATTCCGTGANAAATTTGTTACTAAATNGGCTAAGATATGTGTTCNGGTTTAANGCTNCCGCATAATAGAATAACAATTATAATACTAATAACATCATCCATGTGAGTNTNTTTTTCTTCNNNNNNNTTTTTTTTTTGGAATGAAAATGATCCATGCGAATGTTGGTGTGACAAATAAATNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=33
PRIMER_RIGHT_NUM_RETURNED=33
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=33
PRIMER_PAIR_0_PENALTY=3.037709
PRIMER_LEFT_0_PENALTY=1.305002
PRIMER_RIGHT_0_PENALTY=1.732707
PRIMER_LEFT_0_SEQUENCE=CGATCAAGTGAAAGGACCAA
PRIMER_RIGHT_0_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_0=490,20
PRIMER_RIGHT_0=1408,20
PRIMER_LEFT_0_TM=58.695
PRIMER_RIGHT_0_TM=58.267
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=919
PRIMER_PAIR_1_PENALTY=3.071792
PRIMER_LEFT_1_PENALTY=1.339085
PRIMER_RIGHT_1_PENALTY=1.732707
PRIMER_LEFT_1_SEQUENCE=AGGCCAATTTGCTTTCCAAC
PRIMER_RIGHT_1_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_1=463,20
PRIMER_RIGHT_1=1408,20
PRIMER_LEFT_1_TM=61.339
PRIMER_RIGHT_1_TM=58.267
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=946
PRIMER_PAIR_2_PENALTY=3.149691
PRIMER_LEFT_2_PENALTY=1.416984
PRIMER_RIGHT_2_PENALTY=1.732707
PRIMER_LEFT_2_SEQUENCE=ACGATCAAGTGAAAGGACCAA
PRIMER_RIGHT_2_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_2=489,21
PRIMER_RIGHT_2=1408,20
PRIMER_LEFT_2_TM=59.583
PRIMER_RIGHT_2_TM=58.267
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=920
PRIMER_PAIR_3_PENALTY=3.603331
PRIMER_LEFT_3_PENALTY=1.870624
PRIMER_RIGHT_3_PENALTY=1.732707
PRIMER_LEFT_3_SEQUENCE=ACGATCAAGTGAAAGGACCA
PRIMER_RIGHT_3_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_3=489,20
PRIMER_RIGHT_3=1408,20
PRIMER_LEFT_3_TM=58.129
PRIMER_RIGHT_3_TM=58.267
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=920
PRIMER_PAIR_4_PENALTY=4.532361
PRIMER_LEFT_4_PENALTY=2.799654
PRIMER_RIGHT_4_PENALTY=1.732707
PRIMER_LEFT_4_SEQUENCE=CTCTTGGAATATGGCACCTTT
PRIMER_RIGHT_4_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_4=513,21
PRIMER_RIGHT_4=1408,20
PRIMER_LEFT_4_TM=58.200
PRIMER_RIGHT_4_TM=58.267
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=896
PRIMER_PAIR_5_PENALTY=4.577796
PRIMER_LEFT_5_PENALTY=2.845089
PRIMER_RIGHT_5_PENALTY=1.732707
PRIMER_LEFT_5_SEQUENCE=TCTTGGAATATGGCACCTTT
PRIMER_RIGHT_5_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_5=514,20
PRIMER_RIGHT_5=1408,20
PRIMER_LEFT_5_TM=57.155
PRIMER_RIGHT_5_TM=58.267
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=895
PRIMER_PAIR_6_PENALTY=4.706101
PRIMER_LEFT_6_PENALTY=2.973394
PRIMER_RIGHT_6_PENALTY=1.732707
PRIMER_LEFT_6_SEQUENCE=ACTCTTGGAATATGGCACCTTT
PRIMER_RIGHT_6_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_6=512,22
PRIMER_RIGHT_6=1408,20
PRIMER_LEFT_6_TM=59.027
PRIMER_RIGHT_6_TM=58.267
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=897
PRIMER_PAIR_7_PENALTY=5.051844
PRIMER_LEFT_7_PENALTY=3.319137
PRIMER_RIGHT_7_PENALTY=1.732707
PRIMER_LEFT_7_SEQUENCE=AACTCTTGGAATATGGCACCT
PRIMER_RIGHT_7_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_7=511,21
PRIMER_RIGHT_7=1408,20
PRIMER_LEFT_7_TM=57.681
PRIMER_RIGHT_7_TM=58.267
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=898
PRIMER_PAIR_8_PENALTY=5.398890
PRIMER_LEFT_8_PENALTY=1.305002
PRIMER_RIGHT_8_PENALTY=4.093888
PRIMER_LEFT_8_SEQUENCE=CGATCAAGTGAAAGGACCAA
PRIMER_RIGHT_8_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_8=490,20
PRIMER_RIGHT_8=1431,20
PRIMER_LEFT_8_TM=58.695
PRIMER_RIGHT_8_TM=55.906
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=942
PRIMER_PAIR_9_PENALTY=5.510872
PRIMER_LEFT_9_PENALTY=1.416984
PRIMER_RIGHT_9_PENALTY=4.093888
PRIMER_LEFT_9_SEQUENCE=ACGATCAAGTGAAAGGACCAA
PRIMER_RIGHT_9_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_9=489,21
PRIMER_RIGHT_9=1431,20
PRIMER_LEFT_9_TM=59.583
PRIMER_RIGHT_9_TM=55.906
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=943
PRIMER_PAIR_10_PENALTY=5.548209
PRIMER_LEFT_10_PENALTY=3.815502
PRIMER_RIGHT_10_PENALTY=1.732707
PRIMER_LEFT_10_SEQUENCE=ACTCTTGGAATATGGCACCT
PRIMER_RIGHT_10_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_10=512,20
PRIMER_RIGHT_10=1408,20
PRIMER_LEFT_10_TM=56.184
PRIMER_RIGHT_10_TM=58.267
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=897
PRIMER_PAIR_11_PENALTY=5.757644
PRIMER_LEFT_11_PENALTY=4.024937
PRIMER_RIGHT_11_PENALTY=1.732707
PRIMER_LEFT_11_SEQUENCE=GGACCAATAACTCTTGGAATATGG
PRIMER_RIGHT_11_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_11=503,24
PRIMER_RIGHT_11=1408,20
PRIMER_LEFT_11_TM=59.975
PRIMER_RIGHT_11_TM=58.267
PRIMER_LEFT_11_GC_PERCENT=41.667
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.4000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=906
PRIMER_PAIR_12_PENALTY=5.951579
PRIMER_LEFT_12_PENALTY=1.857691
PRIMER_RIGHT_12_PENALTY=4.093888
PRIMER_LEFT_12_SEQUENCE=AGGATTCGTTGAAAGGCCAA
PRIMER_RIGHT_12_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_12=450,20
PRIMER_RIGHT_12=1431,20
PRIMER_LEFT_12_TM=61.858
PRIMER_RIGHT_12_TM=55.906
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.0000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=982
PRIMER_PAIR_13_PENALTY=5.958248
PRIMER_LEFT_13_PENALTY=4.225541
PRIMER_RIGHT_13_PENALTY=1.732707
PRIMER_LEFT_13_SEQUENCE=TAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_13_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_13=510,22
PRIMER_RIGHT_13=1408,20
PRIMER_LEFT_13_TM=57.774
PRIMER_RIGHT_13_TM=58.267
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=899
PRIMER_PAIR_14_PENALTY=5.964512
PRIMER_LEFT_14_PENALTY=1.870624
PRIMER_RIGHT_14_PENALTY=4.093888
PRIMER_LEFT_14_SEQUENCE=ACGATCAAGTGAAAGGACCA
PRIMER_RIGHT_14_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_14=489,20
PRIMER_RIGHT_14=1431,20
PRIMER_LEFT_14_TM=58.129
PRIMER_RIGHT_14_TM=55.906
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=943
PRIMER_PAIR_15_PENALTY=6.893543
PRIMER_LEFT_15_PENALTY=2.799654
PRIMER_RIGHT_15_PENALTY=4.093888
PRIMER_LEFT_15_SEQUENCE=CTCTTGGAATATGGCACCTTT
PRIMER_RIGHT_15_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_15=513,21
PRIMER_RIGHT_15=1431,20
PRIMER_LEFT_15_TM=58.200
PRIMER_RIGHT_15_TM=55.906
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=919
PRIMER_PAIR_16_PENALTY=6.894607
PRIMER_LEFT_16_PENALTY=2.800718
PRIMER_RIGHT_16_PENALTY=4.093888
PRIMER_LEFT_16_SEQUENCE=TAGGATTCGTTGAAAGGCCAA
PRIMER_RIGHT_16_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_16=449,21
PRIMER_RIGHT_16=1431,20
PRIMER_LEFT_16_TM=61.801
PRIMER_RIGHT_16_TM=55.906
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=10.0000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=983
PRIMER_PAIR_17_PENALTY=6.938977
PRIMER_LEFT_17_PENALTY=2.845089
PRIMER_RIGHT_17_PENALTY=4.093888
PRIMER_LEFT_17_SEQUENCE=TCTTGGAATATGGCACCTTT
PRIMER_RIGHT_17_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_17=514,20
PRIMER_RIGHT_17=1431,20
PRIMER_LEFT_17_TM=57.155
PRIMER_RIGHT_17_TM=55.906
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=918
PRIMER_PAIR_18_PENALTY=7.067283
PRIMER_LEFT_18_PENALTY=2.973394
PRIMER_RIGHT_18_PENALTY=4.093888
PRIMER_LEFT_18_SEQUENCE=ACTCTTGGAATATGGCACCTTT
PRIMER_RIGHT_18_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_18=512,22
PRIMER_RIGHT_18=1431,20
PRIMER_LEFT_18_TM=59.027
PRIMER_RIGHT_18_TM=55.906
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=920
PRIMER_PAIR_19_PENALTY=7.413025
PRIMER_LEFT_19_PENALTY=3.319137
PRIMER_RIGHT_19_PENALTY=4.093888
PRIMER_LEFT_19_SEQUENCE=AACTCTTGGAATATGGCACCT
PRIMER_RIGHT_19_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_19=511,21
PRIMER_RIGHT_19=1431,20
PRIMER_LEFT_19_TM=57.681
PRIMER_RIGHT_19_TM=55.906
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=921
PRIMER_PAIR_20_PENALTY=7.501993
PRIMER_LEFT_20_PENALTY=5.769286
PRIMER_RIGHT_20_PENALTY=1.732707
PRIMER_LEFT_20_SEQUENCE=AGGACCAATAACTCTTGGAATATGG
PRIMER_RIGHT_20_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_20=502,25
PRIMER_RIGHT_20=1408,20
PRIMER_LEFT_20_TM=60.769
PRIMER_RIGHT_20_TM=58.267
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.4000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=907
PRIMER_PAIR_21_PENALTY=7.909391
PRIMER_LEFT_21_PENALTY=3.815502
PRIMER_RIGHT_21_PENALTY=4.093888
PRIMER_LEFT_21_SEQUENCE=ACTCTTGGAATATGGCACCT
PRIMER_RIGHT_21_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_21=512,20
PRIMER_RIGHT_21=1431,20
PRIMER_LEFT_21_TM=56.184
PRIMER_RIGHT_21_TM=55.906
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=920
PRIMER_PAIR_22_PENALTY=8.118825
PRIMER_LEFT_22_PENALTY=4.024937
PRIMER_RIGHT_22_PENALTY=4.093888
PRIMER_LEFT_22_SEQUENCE=GGACCAATAACTCTTGGAATATGG
PRIMER_RIGHT_22_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_22=503,24
PRIMER_RIGHT_22=1431,20
PRIMER_LEFT_22_TM=59.975
PRIMER_RIGHT_22_TM=55.906
PRIMER_LEFT_22_GC_PERCENT=41.667
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.4000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=929
PRIMER_PAIR_23_PENALTY=8.319430
PRIMER_LEFT_23_PENALTY=4.225541
PRIMER_RIGHT_23_PENALTY=4.093888
PRIMER_LEFT_23_SEQUENCE=TAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_23_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_23=510,22
PRIMER_RIGHT_23=1431,20
PRIMER_LEFT_23_TM=57.774
PRIMER_RIGHT_23_TM=55.906
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=922
PRIMER_PAIR_24_PENALTY=8.402475
PRIMER_LEFT_24_PENALTY=6.669768
PRIMER_RIGHT_24_PENALTY=1.732707
PRIMER_LEFT_24_SEQUENCE=CAATAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_24_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_24=507,25
PRIMER_RIGHT_24=1408,20
PRIMER_LEFT_24_TM=61.670
PRIMER_RIGHT_24_TM=58.267
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=902
PRIMER_PAIR_25_PENALTY=8.680999
PRIMER_LEFT_25_PENALTY=4.587110
PRIMER_RIGHT_25_PENALTY=4.093888
PRIMER_LEFT_25_SEQUENCE=CTAGGATTCGTTGAAAGGCCAA
PRIMER_RIGHT_25_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_25=448,22
PRIMER_RIGHT_25=1431,20
PRIMER_LEFT_25_TM=62.587
PRIMER_RIGHT_25_TM=55.906
PRIMER_LEFT_25_GC_PERCENT=45.455
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=10.0000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=984
PRIMER_PAIR_26_PENALTY=9.863175
PRIMER_LEFT_26_PENALTY=5.769286
PRIMER_RIGHT_26_PENALTY=4.093888
PRIMER_LEFT_26_SEQUENCE=AGGACCAATAACTCTTGGAATATGG
PRIMER_RIGHT_26_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_26=502,25
PRIMER_RIGHT_26=1431,20
PRIMER_LEFT_26_TM=60.769
PRIMER_RIGHT_26_TM=55.906
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=930
PRIMER_PAIR_27_PENALTY=10.763656
PRIMER_LEFT_27_PENALTY=6.669768
PRIMER_RIGHT_27_PENALTY=4.093888
PRIMER_LEFT_27_SEQUENCE=CAATAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_27_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_27=507,25
PRIMER_RIGHT_27=1431,20
PRIMER_LEFT_27_TM=61.670
PRIMER_RIGHT_27_TM=55.906
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=925
PRIMER_PAIR_28_PENALTY=11.228153
PRIMER_LEFT_28_PENALTY=7.134264
PRIMER_RIGHT_28_PENALTY=4.093888
PRIMER_LEFT_28_SEQUENCE=TCTAGGATTCGTTGAAAGGCCAA
PRIMER_RIGHT_28_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_28=447,23
PRIMER_RIGHT_28=1431,20
PRIMER_LEFT_28_TM=64.134
PRIMER_RIGHT_28_TM=55.906
PRIMER_LEFT_28_GC_PERCENT=43.478
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=10.0000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=985
PRIMER_PAIR_29_PENALTY=12.129367
PRIMER_LEFT_29_PENALTY=10.396660
PRIMER_RIGHT_29_PENALTY=1.732707
PRIMER_LEFT_29_SEQUENCE=CCAATAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_29_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_29=506,26
PRIMER_RIGHT_29=1408,20
PRIMER_LEFT_29_TM=64.397
PRIMER_RIGHT_29_TM=58.267
PRIMER_LEFT_29_GC_PERCENT=42.308
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=903
PRIMER_PAIR_30_PENALTY=13.628643
PRIMER_LEFT_30_PENALTY=11.895936
PRIMER_RIGHT_30_PENALTY=1.732707
PRIMER_LEFT_30_SEQUENCE=ACCAATAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_30_SEQUENCE=CAACGTTCAATTGCTTTTCC
PRIMER_LEFT_30=505,27
PRIMER_RIGHT_30=1408,20
PRIMER_LEFT_30_TM=64.896
PRIMER_RIGHT_30_TM=58.267
PRIMER_LEFT_30_GC_PERCENT=40.741
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=904
PRIMER_PAIR_31_PENALTY=14.490549
PRIMER_LEFT_31_PENALTY=10.396660
PRIMER_RIGHT_31_PENALTY=4.093888
PRIMER_LEFT_31_SEQUENCE=CCAATAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_31_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_31=506,26
PRIMER_RIGHT_31=1431,20
PRIMER_LEFT_31_TM=64.397
PRIMER_RIGHT_31_TM=55.906
PRIMER_LEFT_31_GC_PERCENT=42.308
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=926
PRIMER_PAIR_32_PENALTY=15.989824
PRIMER_LEFT_32_PENALTY=11.895936
PRIMER_RIGHT_32_PENALTY=4.093888
PRIMER_LEFT_32_SEQUENCE=ACCAATAACTCTTGGAATATGGCACCT
PRIMER_RIGHT_32_SEQUENCE=GAGATTGGAAATCAACCACA
PRIMER_LEFT_32=505,27
PRIMER_RIGHT_32=1431,20
PRIMER_LEFT_32_TM=64.896
PRIMER_RIGHT_32_TM=55.906
PRIMER_LEFT_32_GC_PERCENT=40.741
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=927
=
SEQUENCE_ID=MAGIC.149_1_12621185_12621626_kn-0_no-0_150359_43042_TRUE
SEQUENCE_TARGET=950,541
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAACTGGATCTATTTTTAAGCNATAATCATGTACCGTATCAACANTGNTTTACCATGTTTAACCATAATACAANNNNTATAGNTAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_10524208_10525000_ler-0_rsch-4_50344_37715_TRUE
SEQUENCE_TARGET=950,892
SEQUENCE_TEMPLATE=CCATTTGGCAATGGAACCCACTCGTGTCCTGGAAATGAATTAGCCAAGCTTGAGATGTCTATTATGATTCATCATCTCACCACCAAGTACAGNNGTGTGTGTGTGTACTACTTACTTATTACCTTCTCNNTCACTCATTTTGTTNTTTTCTTTCAAATTTATGTGTAGAATTGTCTTTGATANGGGGTATATTTGTAATTTCAGTTGGTCAATNGTTGGAGCGAGCGACGGGATTCAGTATGGGCCATTCGCGCTTCCCCAAAACGGACTGCCCATTGTGCTGGCCCGGAAGCCGGAGATCGAAGTGTAGAATGACAGAATTGCCTTTAGCTTTNCTATATTTGGAAAGAGNAGACTAGAGAAGAAGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAANATAGAGATGGAGGAAAACAGGTTCAAGATTTTGGTAGAAAAATATTCCCCAAATGGGATCAAGGAGAAAACACCAAGTGTACAAAANCTTCAATTTTATTTTTCTTGACTTNCTTTANTTTTTTTTTTTTGAGNTTTTAATTTTAATTTAAGATGGGAAAAGCTAGTAATTGAATTTNAAAAAACAGGGGAAAATGTTAGTACAAGTCTCTCATGTANTTTTTTTTTGTTTTTCCTGAAAATATCTTATGGTTATGTTATAACTATTTCCTCCTGGATTGAGACTTCCCTTGTTTTTTGAATNNATTTTTTTTGTTATATNNNNATATATATNTTNAAAATATCTGTTTTAATGAGCCGTATTGGTTATATACTTATATTGAAGANATCCCAAAGCAATATTCTACAATTTGGTGATTGCTTCTATATTACAAGAAATAACTTGTCTTACAAGTCTTGAATCTAAATATCTAAAAGTCAAACTATTAAAAATATTATTCAAATCAAGATTATTGTTCATATATGTTCTGTATACGATCGTTACTTATATAATGTAGAAATTATATATGTGTATGGGGTTTCCGNAATATTGGAATTNCAACATGCTTCAAAGTCCTGATGGCTAATTGGGTCAAGGAAACTACATTCCAAAATAAACTGGTTTTGACCNTACCGGCTACATGAATCTCTCAAGATGCACTAAGNACGTTATTAACNAGTAAATATGGGTATCTCCGTATCTGACTATCTGAGACAAAGTTAATTATCCTACACGATCGCAAAAGTTTTTACCTGNTTTTTTTTTTTACAACCAGTGTCCAGTGATTATTCAAAATTTTAGATTACTACAAATGTGATTGATCGTTACAAAAATAAAAGTTTTGNNNNNNNNNNAANAAAAAAAAAGAATTTCACNCTATATTTAACCACACNTACCACATTTTTTTCTATAGACTTAGGAACCACATAAATGATGACTATAGAATTGCCCTAATCATTATAGACAGTTTTTTTGNAAAAAGGTTAATTTATTAGNTTGACGTGAAAAAAAGAAACCCCGACTTTTNTTCAAATAATTTGACGGACGACTTTGACCATCATCACCCGTGAATTATGTTTGTGGCCATCNTCGCTGTAATTATATTACTTTTTCNACGTATTATTATTTTATCTAAATTTTCACTGGTGATATGTATATAAATCATCTTTTATGATATTATATATACCATGATTGAGAATATTGATTATTGAGGAACAGCTGTTATTGCTTCCTCGATGAACAAATATCAAACGGGCTATCAATTTCTAGTCACTTTCTTCCGTCTNNNNTATTNTCGTTTNNNNNNNNNTTATTTTTATATTCAGATATTTTCTTTCTGAAGAATTTGTNTTTAAAGCANATAGTNTAATATCTNAAAGGCTTAAAGGCATTGGACAAGNGAACCAACTACATAGNNNNNNNNNNNNNNNNNNNNNNAACATTTCTTCTCATATATAACCAAAAAATCCTATGCGTACGTTTTCTCTGTAAATTTTAACTAATTGTAGAACTATATCTTATATATTACAGATAGATGAAAAATGATGAGATATGATTANAAAAAAATCATACGTGATTAGTATATACTCCTAAACAAAGATCGTTTGAGCATTNTTATTATGTTTACTTGTACTTTTGACTTTGNATTACTTTACGTCATATTGTCGTCCTTTAAATGTACCATGCTCCATGATTTTCACGTTCATCTTCTAAATCTAGAATAATGTAGATATTACATTATCGATGGTCCCGNATATGCCGGTCTGTNTNATGAGATTGTATTATTATGNTTTTNTTACAAGTGTTTATATATGTNTGGAGAACTTGCATATGTGAATAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_24175133_24175755_can-0_mt-0_390128_1475_TRUE
SEQUENCE_TARGET=950,722
SEQUENCE_TEMPLATE=GTCTTAGTTGCCTGAAGCTTTAGNTTATTTCTTTCTTCCATAAGGGACTGAAAGAGTGAGCCTTTCCGCTCTACATCCTGCTCNGGATTCAATTTCTCCGTCCACTTATGCGCATTCTCTAATATTCTTTTATTGAAACCAATGGACTTAGCTANTCTCAATGCATTTGATAGACCAGTACTTCCCCAGAGTACTCGAAATGTAGGCTGCAAAGTTTCCATGGAGAACTCCATTGCAGCATTTTGGAATCGAGGNTCATTATNCTTCAANCGACTTAAGTCACCGTAATGTGTTGATACAACNGCCACATTAACACGGTTNTTAATATACTGTAGGATNCTAGTAGCAAGTGCAACCCCTTCTGAAGGATCAGTTCCACTACATATTTCGTCTAGTAANACAAGNGAATTTTCTGAAGCAATGTCAANTATCTGNCGTATCCGTGAGATGTGNCCACTAAAGGTTGANAGACTTTGCTCCAAAGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNTTGCAAGGAATTCANAANGTAATACCTGNGGATCNCCNATNTCAGCCAGAATAAGATCAAACCAAGGCAACCTTGGGCAGTTCTTAGCAGGCAAATACATNCCGGACTTNGACATAAGAGACAATAATCCCAATGTCTTTAANAAAGCNGTCTTNCCTCCTGTGTTTGGACCCGAGATGACGACTACTTTGGCACTGCTTTCAACTTTAATNTCTACTGGCACAGGGAAAATGTCTCCACCNTTTGGNCTGCCTAATACTGAACCAAGAAGTAGCGGGTGCTGCGCAGAATCAATGTCTACAGCTAAGCCCNGTGTTTTNGTGTGTTCTGAAGTTACGTTGGGATAGACACCATTAATCCANNTTGCATGTGATGCTCTTGCAAAAGCAATGTCAAGTTCTAATATTCTATCCAACAAATNCAATATNTCNCTCTGCGCCATNACCACCTCNGATGTCAAAATGCNCAAAATCGCCNTTTCCTCAGCTTTCTCNNAGTTNGCATGTCTCACTTCCATATTATTAAGCTCCACTGCCTCTTTCGGTTCTATNAANCATGTTGCCCTNGAGCTACTAACACTTAAAACAACACCACCTGGAAGTAAACTTTTGTGTGTNGCTCTAANAGCTACACACATCCTTGATCTCCTCTGCGTTATCAAAGGCTTGTTAATCCCCCCAGCTAGAAAAATCTTNGTNGATATTTTCTTCAAAAGAGAATCCAAANTCTCCATGTTTCTNCTCCGTTCAGATCTTATAATCTCTAAATCTTCACTAGCTCTATCAAGAATCATAGTCATNTTGCAGTCNATACAAAAACTAATCTTCTGCTGTAATGTATCCTTGAAATCACAACCCTGTAGTATATCTACAAGTGGAGTAACCCTACAAAATAANAGAAGNAANAGCTAGAACCATNAGCNTCTCAATAGCAATANGAACANCTAATAACTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNTNNCNNNNNNNNTNNNNNGNNNNNNNANNNNCNNNNNNCNNNNNNNNNANNNTNCNNNNNTNTCNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNCGNNNNNNNNNNNNNNNNNNNNNNGNNNNTNNNGNNNNNNNNNNGNNNNNGAGNNNNCNNNNNNNNNNNGGCNNNNNNNNNNNNNGANNNNNNNNNNNNNNNGTNNNNGNNNNNCNNNNNNNNGNNNNNGNNNNNGNNNNNNNNNNANNNNANNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNGNCNGNNNGNGNNNNGNNNNNNGNNGCGNCGCGNNNNNNNNNNNNNGNNNNNNNNCGNNNNNANNNNNNNNNGGNNNNNNGTGNNNANTTGNGNTGAATNNGNNTNNNNATNNNTTNNNNNNNNNNANNAGTNTTNANNNCTGTGGNNAANGANNNNTCNANNNTNCNNTTNNNNTNTGNNNNNNNTCNGNNNNTNNNNAGTTNNNNNNNNTTNNNTNNGGANNNNNNNNTTGNNCNNNTTAANNNANNTTGTTCNNGNTTNAANNNNTNNNNNANTNNNNTTCGNTNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNTNNNNNNNNNTNNNNNANNNNNNNNNNNNNTNNNNANCNANNNNNNNNNNNTNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNTNNNNAGNNNNNNNNNNNNNNTNNNNNTNNNNNTNTNNGNNNNANNNNNNNGNNNNNNNCNNNNNTNNNNGNNNNNNNNNNNTNNNNNCNCNNCNNCNCCNNTNNTCNNCNNTNNNCTNCTCNCNNNNNCNCCCNCNNCNTNNNNNNCTCNNNNTNNNNCTCNNNTNCNCNNNCNCNCNNNTNNCCNNNNNNNNCNNNNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_8974725_8974775_kn-0_ler-0_177664_82381_TRUE
SEQUENCE_TARGET=950,150
SEQUENCE_TEMPLATE=TGTGGATGTNGAAANTACCTTTCCAGAGTCAAGAGGAATATCCATTGTGGCACCACGGTCGTACGGCTCAGGGGGAAGAGGAGAAAGCTTTGACGTTGCAGCTGGNACACTTGTAGGATTCTGAAGGTTCAAANNCAAGTAAGTAAACCATTACACCATATCCAAGATCAGTANAANGTATCAATGGCCACATGTAAGCACTAAACTGGAAATGGAAAAAGATGTAAGTGANATTAATAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCAATTACATACTGAAACTTGTTATCACCCTTCCCAGAGACACTCACNTCACAAAGNCCAACAATCATACGAACACAAGAAANNNNNNNNNNNNGCGTAAATCAATCAANCGATCAACNACGTTTCNTCCTTGTGCATTGAAACCGAAAAGTCNNTCCCAAGAATTGCGAGTAACANTACGTACACGTTACAACANGAATGTGANAAATCAGATCAGAAACANTGCGATTCAACAAAANCGAATAAAAAGTANATCCCNNNNNNNNNNNNNTGATGATGNGATTGAATCAAGCTTACCGCAAAAGGATTAANNTCNCCGTCATCAAAGGAATGAGACTGGTAGCGAGACATTGCACCGTTACTCAGCANATNCAATCTCTCTACACGGATCACACCGAGCNNNNNNNNNNNNNNGAGAGAGAGAGCGATTGGAGATGAAGCAATNGGGTNNGCGTTTTAGAGTAATAAGGAAGTGTTTGGCGCGTCTGGGTTATACCCGGTTTTNTAANCCNGAGATAANCAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTCAGAGATTGCTAATCCTAATCCGGTGGAAATACATTNNGGGTGTTGTGGATAGACTAAAAGGCNAAAGCAGAGTAANCTGTGTGCCTGTGNNNNNNTNNCACACTCTACNNATNTAATAATACAAGCAAGGCTCGTTAAAGAACAATGGTTTCGACTTTCTANAGAATACTAATAATACCACTTGTTTTGTATAAGAGACAATCTCCTTAAACTATCTTCTTCNCGTATACTGATTATCCCCATTTGAGTCATTTCTCCGATGTTCTCTCTGGATCATGTCTGAGAGGTCTTGAACCACTTCTGACATTAGTGGCCT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_12153205_12153822_oy-0_ct-1_44917_168920_TRUE
SEQUENCE_TARGET=950,717
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17155009_17155473_mt-0_edi-0_48954_12670_TRUE
SEQUENCE_TARGET=950,564
SEQUENCE_TEMPLATE=ATATCTATTTATTTNNTTNTTTTTTGGGTACGTCGTCCTTACTTTGCCCCTCTTTATCTCCAATCTCTGCAACATTCCTCATTATTAATTTCATCTTTACATCTCTCCCCCNCTCTCTCNTNNNNNNNNNNNNTGTATCCTGCTTGAACTCANTTCCAACCAGAAACCATATNTCTTTTAGTTGTGTCCTTCACCCTTGGAGAGTTGNAAAAAAGGAGGATGTTTGACTTGCAATTCAAGGCACGTTCCAGAGGCTAAACTACGTGAACTTCAGGTGATCTAATCGTTTCTTTGTTTCCCCTTCTTGAATATTTTTTGGTTTACCAAAGTGAACGNGTCATATATGGATAATATTACATAGTAGTGTATAATTGCCCGGAAATTAGGATTAAGTAAGCTAATTACACATTGTTATGGTTGCCAAATTCCGGGTGGAGAACATGTGCGCAAGCAGATTAGCNAGAAAAGCTTACATATTTTATGAGTGAGTCATTTNTAATCAATTGATATTAATGCGCATACTGCGACCGATTTAGGCTCTAGGAAATATGATTACTATCTGATAAATTTANCAAAATGCTAATCTTTGGTATTAATTGCACACATAGATNAATGGCGTTAAACTGGTGTGACAAAGAAGATTAGTGTTTAATGTTTTCCTACATNTATATGCGATTCACCATAATATTTATAAGGTAANTAAAAGTACTATCAATCTGATAAACTTTTTCCAAGCCTTTGAGTGAGCACCTTTTTAGTTTACACTTGATAAGTCTTCTTGGATTACTCATAACAAAAACAAACAAAAATATTGGATTAGAAAACCATGATGATCTAGATGGTCTTGCGTGNGAAAACATACTAATTAATTAGGTTCTACGATCATATCTATCTAGATGATCAACATTTATTAACTCTAGGAAAGGTTTTATTGGGAGATTTTTTGAGTAAATCTTACGAATAATTAATTTTACATAACAGTAAGCTCTAAAACTAATTGNTTTTTTCTTCCATAGGCACGTGATGCAGTAACGTACGAATTCTATTATTAATCCACGAGTTGCTTCATATATNTATTCGTCATTTATTCTTCAGACACCAATTCATTGTGAAGNAGAACACTACGAGTGTAACAATAGGACCAATACTATAATCGTAAGTTCGTAACTTATATTTTTAACAACTTTGCCCTCATTTGCTTTCCTGTCNTTGTCACTATTTAATTCACGCTAATCAATTTACCTTCAAATCTATATAGATTAACTTTACAGTANCAATATAAAATGTTGATGCTATATGAAGATATTGTTCTGAGTATCAAATCATGTAGGCTTCACTAGACCTTATGATAATATTATAGTTTACCTATTATTATAACGTAGAATTTCTTGAGTTTATCCAATGGGAGAAGCAACCGGCAACATCGGCATAAACATGGNAAAAAAACGTATCGAGAACCAAAAANACTTACTTGTTTATATAAAACCCACAAATACTAAAATTTATAATAAAGATTCATTACCGTACAATTTAAATATTAAATNNACACATACATTATACATGACAACAAACAGACATACAAATGTTTAAATCGTCTAAGTATTACATATGATTGACCAGAGCAACAAAACAAAAGACACATTACATATATAGAATCACTATATAACAAAAATAAAAAGTNAAGTTCTTGATTTAATCAAGATCTTGGTGGATTTTGATAGAATTGCGTACCTGGTGGCATGAACACACTAGTCGTCATGTTACTTGAATAGTAACCACCAGAATTTGGATCTACAAACATGGCCGATGATACTCCTCCGCCACCGTCGGTCACAACTGGAGGATTAAACGGATGTCCTCCTCCTCCGCCTCCTGCGGATGAGCTCTGATGATCGCTTCCNCCACTACCGCCACTCATGTAGCTGCTTTCAGGGCCTTGGTCATACAGAATTCCTTTGAAGAGATGACCTCCAATATTGACCGTCGTTTGATAAGCGTACTGACCATCTCCTCCATCATCTACGCCACTCATTTTAACACACCGAAAAAGCGCATCTGAGCTCACTTCCCCCGGAAAACTTGCCTCCCCCATTTCTAACCCTAAAAANATATATACACAATTATACACATTAATCAACGTCCATAAACACAAGATTACGTTTATGTTTTAATGTTATGTTTCACTTCAAAACAGAACACAACATTNAAATGNNTTTTANCAAACATTTTTTCACACTGAAATTTGATTAAAAATAAATATAGAATGTTGTATTTTTAAAGTAAACAAAATTTGTGCAAGTAGTGAAAGGAAAATTAAACTCACATATATTTTTGTGTTGAAAAATTCGTGTTTCTTCTAACAAAAACATATGGTTCGNATATATGAAGTTGTCTGGATATNAAATTGTTTATAAAGANATNAAAAAAAAAAACCTGCTGAGTNGGATGGCAAGCGAGAAGAGGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.676466
PRIMER_LEFT_0_PENALTY=0.522443
PRIMER_RIGHT_0_PENALTY=2.154023
PRIMER_LEFT_0_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_0_SEQUENCE=TGTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_0=734,20
PRIMER_RIGHT_0=1631,22
PRIMER_LEFT_0_TM=59.478
PRIMER_RIGHT_0_TM=60.154
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=898
PRIMER_PAIR_1_PENALTY=3.063759
PRIMER_LEFT_1_PENALTY=0.909736
PRIMER_RIGHT_1_PENALTY=2.154023
PRIMER_LEFT_1_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_1_SEQUENCE=TGTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_1=733,20
PRIMER_RIGHT_1=1631,22
PRIMER_LEFT_1_TM=59.090
PRIMER_RIGHT_1_TM=60.154
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=899
PRIMER_PAIR_2_PENALTY=3.195128
PRIMER_LEFT_2_PENALTY=0.522443
PRIMER_RIGHT_2_PENALTY=2.672685
PRIMER_LEFT_2_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_2_SEQUENCE=CGCAATTCTATCAAAATCCACC
PRIMER_LEFT_2=734,20
PRIMER_RIGHT_2=1727,22
PRIMER_LEFT_2_TM=59.478
PRIMER_RIGHT_2_TM=60.673
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=994
PRIMER_PAIR_3_PENALTY=3.582421
PRIMER_LEFT_3_PENALTY=0.909736
PRIMER_RIGHT_3_PENALTY=2.672685
PRIMER_LEFT_3_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_3_SEQUENCE=CGCAATTCTATCAAAATCCACC
PRIMER_LEFT_3=733,20
PRIMER_RIGHT_3=1727,22
PRIMER_LEFT_3_TM=59.090
PRIMER_RIGHT_3_TM=60.673
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=995
PRIMER_PAIR_4_PENALTY=3.583698
PRIMER_LEFT_4_PENALTY=1.429676
PRIMER_RIGHT_4_PENALTY=2.154023
PRIMER_LEFT_4_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_4_SEQUENCE=TGTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_4=733,21
PRIMER_RIGHT_4=1631,22
PRIMER_LEFT_4_TM=60.430
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=899
PRIMER_PAIR_5_PENALTY=3.583698
PRIMER_LEFT_5_PENALTY=1.429676
PRIMER_RIGHT_5_PENALTY=2.154023
PRIMER_LEFT_5_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_5_SEQUENCE=TGTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_5=732,21
PRIMER_RIGHT_5=1631,22
PRIMER_LEFT_5_TM=60.430
PRIMER_RIGHT_5_TM=60.154
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=900
PRIMER_PAIR_6_PENALTY=3.595050
PRIMER_LEFT_6_PENALTY=0.522443
PRIMER_RIGHT_6_PENALTY=3.072607
PRIMER_LEFT_6_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_6_SEQUENCE=CTTTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_6=734,20
PRIMER_RIGHT_6=1635,22
PRIMER_LEFT_6_TM=59.478
PRIMER_RIGHT_6_TM=58.927
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=902
PRIMER_PAIR_7_PENALTY=3.595050
PRIMER_LEFT_7_PENALTY=0.522443
PRIMER_RIGHT_7_PENALTY=3.072607
PRIMER_LEFT_7_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_7_SEQUENCE=GTCTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_7=734,20
PRIMER_RIGHT_7=1637,22
PRIMER_LEFT_7_TM=59.478
PRIMER_RIGHT_7_TM=58.927
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=904
PRIMER_PAIR_8_PENALTY=3.941277
PRIMER_LEFT_8_PENALTY=1.787254
PRIMER_RIGHT_8_PENALTY=2.154023
PRIMER_LEFT_8_SEQUENCE=GCCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_8_SEQUENCE=TGTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_8=734,21
PRIMER_RIGHT_8=1631,22
PRIMER_LEFT_8_TM=60.787
PRIMER_RIGHT_8_TM=60.154
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=898
PRIMER_PAIR_9_PENALTY=3.982343
PRIMER_LEFT_9_PENALTY=0.909736
PRIMER_RIGHT_9_PENALTY=3.072607
PRIMER_LEFT_9_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_9_SEQUENCE=CTTTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_9=733,20
PRIMER_RIGHT_9=1635,22
PRIMER_LEFT_9_TM=59.090
PRIMER_RIGHT_9_TM=58.927
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=903
PRIMER_PAIR_10_PENALTY=3.982343
PRIMER_LEFT_10_PENALTY=0.909736
PRIMER_RIGHT_10_PENALTY=3.072607
PRIMER_LEFT_10_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_10_SEQUENCE=GTCTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_10=733,20
PRIMER_RIGHT_10=1637,22
PRIMER_LEFT_10_TM=59.090
PRIMER_RIGHT_10_TM=58.927
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=905
PRIMER_PAIR_11_PENALTY=4.102361
PRIMER_LEFT_11_PENALTY=1.429676
PRIMER_RIGHT_11_PENALTY=2.672685
PRIMER_LEFT_11_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_11_SEQUENCE=CGCAATTCTATCAAAATCCACC
PRIMER_LEFT_11=733,21
PRIMER_RIGHT_11=1727,22
PRIMER_LEFT_11_TM=60.430
PRIMER_RIGHT_11_TM=60.673
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=995
PRIMER_PAIR_12_PENALTY=4.102361
PRIMER_LEFT_12_PENALTY=1.429676
PRIMER_RIGHT_12_PENALTY=2.672685
PRIMER_LEFT_12_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_12_SEQUENCE=CGCAATTCTATCAAAATCCACC
PRIMER_LEFT_12=732,21
PRIMER_RIGHT_12=1727,22
PRIMER_LEFT_12_TM=60.430
PRIMER_RIGHT_12_TM=60.673
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=996
PRIMER_PAIR_13_PENALTY=4.129843
PRIMER_LEFT_13_PENALTY=0.522443
PRIMER_RIGHT_13_PENALTY=3.607400
PRIMER_LEFT_13_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_13_SEQUENCE=TTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_13=734,20
PRIMER_RIGHT_13=1633,20
PRIMER_LEFT_13_TM=59.478
PRIMER_RIGHT_13_TM=56.393
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=900
PRIMER_PAIR_14_PENALTY=4.203924
PRIMER_LEFT_14_PENALTY=0.522443
PRIMER_RIGHT_14_PENALTY=3.681481
PRIMER_LEFT_14_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_14_SEQUENCE=TTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_14=734,20
PRIMER_RIGHT_14=1629,20
PRIMER_LEFT_14_TM=59.478
PRIMER_RIGHT_14_TM=56.319
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=896
PRIMER_PAIR_15_PENALTY=4.211355
PRIMER_LEFT_15_PENALTY=0.522443
PRIMER_RIGHT_15_PENALTY=3.688912
PRIMER_LEFT_15_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_15_SEQUENCE=GTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_15=734,20
PRIMER_RIGHT_15=1630,21
PRIMER_LEFT_15_TM=59.478
PRIMER_RIGHT_15_TM=57.311
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=897
PRIMER_PAIR_16_PENALTY=4.246410
PRIMER_LEFT_16_PENALTY=4.215367
PRIMER_RIGHT_16_PENALTY=0.031043
PRIMER_LEFT_16_SEQUENCE=GGAAAGGTTTTATTGGGAGA
PRIMER_RIGHT_16_SEQUENCE=ATCCTCCAGTTGTGACCGAC
PRIMER_LEFT_16=919,20
PRIMER_RIGHT_16=1844,20
PRIMER_LEFT_16_TM=55.785
PRIMER_RIGHT_16_TM=59.969
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=926
PRIMER_PAIR_17_PENALTY=4.399283
PRIMER_LEFT_17_PENALTY=0.522443
PRIMER_RIGHT_17_PENALTY=3.876840
PRIMER_LEFT_17_SEQUENCE=GCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_17_SEQUENCE=CTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_17=734,20
PRIMER_RIGHT_17=1635,20
PRIMER_LEFT_17_TM=59.478
PRIMER_RIGHT_17_TM=56.123
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=902
PRIMER_PAIR_18_PENALTY=4.459939
PRIMER_LEFT_18_PENALTY=1.787254
PRIMER_RIGHT_18_PENALTY=2.672685
PRIMER_LEFT_18_SEQUENCE=GCCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_18_SEQUENCE=CGCAATTCTATCAAAATCCACC
PRIMER_LEFT_18=734,21
PRIMER_RIGHT_18=1727,22
PRIMER_LEFT_18_TM=60.787
PRIMER_RIGHT_18_TM=60.673
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=994
PRIMER_PAIR_19_PENALTY=4.502283
PRIMER_LEFT_19_PENALTY=1.429676
PRIMER_RIGHT_19_PENALTY=3.072607
PRIMER_LEFT_19_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_19_SEQUENCE=CTTTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_19=733,21
PRIMER_RIGHT_19=1635,22
PRIMER_LEFT_19_TM=60.430
PRIMER_RIGHT_19_TM=58.927
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=903
PRIMER_PAIR_20_PENALTY=4.502283
PRIMER_LEFT_20_PENALTY=1.429676
PRIMER_RIGHT_20_PENALTY=3.072607
PRIMER_LEFT_20_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_20_SEQUENCE=GTCTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_20=733,21
PRIMER_RIGHT_20=1637,22
PRIMER_LEFT_20_TM=60.430
PRIMER_RIGHT_20_TM=58.927
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=905
PRIMER_PAIR_21_PENALTY=4.502283
PRIMER_LEFT_21_PENALTY=1.429676
PRIMER_RIGHT_21_PENALTY=3.072607
PRIMER_LEFT_21_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_21_SEQUENCE=CTTTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_21=732,21
PRIMER_RIGHT_21=1635,22
PRIMER_LEFT_21_TM=60.430
PRIMER_RIGHT_21_TM=58.927
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=904
PRIMER_PAIR_22_PENALTY=4.502283
PRIMER_LEFT_22_PENALTY=1.429676
PRIMER_RIGHT_22_PENALTY=3.072607
PRIMER_LEFT_22_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_22_SEQUENCE=GTCTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_22=732,21
PRIMER_RIGHT_22=1637,22
PRIMER_LEFT_22_TM=60.430
PRIMER_RIGHT_22_TM=58.927
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=906
PRIMER_PAIR_23_PENALTY=4.517136
PRIMER_LEFT_23_PENALTY=0.909736
PRIMER_RIGHT_23_PENALTY=3.607400
PRIMER_LEFT_23_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_23_SEQUENCE=TTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_23=733,20
PRIMER_RIGHT_23=1633,20
PRIMER_LEFT_23_TM=59.090
PRIMER_RIGHT_23_TM=56.393
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=901
PRIMER_PAIR_24_PENALTY=4.591217
PRIMER_LEFT_24_PENALTY=0.909736
PRIMER_RIGHT_24_PENALTY=3.681481
PRIMER_LEFT_24_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_24_SEQUENCE=TTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_24=733,20
PRIMER_RIGHT_24=1629,20
PRIMER_LEFT_24_TM=59.090
PRIMER_RIGHT_24_TM=56.319
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=897
PRIMER_PAIR_25_PENALTY=4.598648
PRIMER_LEFT_25_PENALTY=0.909736
PRIMER_RIGHT_25_PENALTY=3.688912
PRIMER_LEFT_25_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_25_SEQUENCE=GTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_25=733,20
PRIMER_RIGHT_25=1630,21
PRIMER_LEFT_25_TM=59.090
PRIMER_RIGHT_25_TM=57.311
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=898
PRIMER_PAIR_26_PENALTY=4.655836
PRIMER_LEFT_26_PENALTY=2.501813
PRIMER_RIGHT_26_PENALTY=2.154023
PRIMER_LEFT_26_SEQUENCE=CCTTTGAGTGAGCACCTTTTT
PRIMER_RIGHT_26_SEQUENCE=TGTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_26=735,21
PRIMER_RIGHT_26=1631,22
PRIMER_LEFT_26_TM=58.498
PRIMER_RIGHT_26_TM=60.154
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.6000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=897
PRIMER_PAIR_27_PENALTY=4.670914
PRIMER_LEFT_27_PENALTY=4.215367
PRIMER_RIGHT_27_PENALTY=0.455547
PRIMER_LEFT_27_SEQUENCE=GGAAAGGTTTTATTGGGAGA
PRIMER_RIGHT_27_SEQUENCE=GAGGAGGAGGACATCCGTTT
PRIMER_LEFT_27=919,20
PRIMER_RIGHT_27=1865,20
PRIMER_LEFT_27_TM=55.785
PRIMER_RIGHT_27_TM=60.456
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=8.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=947
PRIMER_PAIR_28_PENALTY=4.725698
PRIMER_LEFT_28_PENALTY=4.215367
PRIMER_RIGHT_28_PENALTY=0.510331
PRIMER_LEFT_28_SEQUENCE=GGAAAGGTTTTATTGGGAGA
PRIMER_RIGHT_28_SEQUENCE=AATCCTCCAGTTGTGACCGA
PRIMER_LEFT_28=919,20
PRIMER_RIGHT_28=1845,20
PRIMER_LEFT_28_TM=55.785
PRIMER_RIGHT_28_TM=60.510
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=9.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=927
PRIMER_PAIR_29_PENALTY=4.786576
PRIMER_LEFT_29_PENALTY=0.909736
PRIMER_RIGHT_29_PENALTY=3.876840
PRIMER_LEFT_29_SEQUENCE=AGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_29_SEQUENCE=CTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_29=733,20
PRIMER_RIGHT_29=1635,20
PRIMER_LEFT_29_TM=59.090
PRIMER_RIGHT_29_TM=56.123
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=903
PRIMER_PAIR_30_PENALTY=4.859861
PRIMER_LEFT_30_PENALTY=1.787254
PRIMER_RIGHT_30_PENALTY=3.072607
PRIMER_LEFT_30_SEQUENCE=GCCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_30_SEQUENCE=CTTTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_30=734,21
PRIMER_RIGHT_30=1635,22
PRIMER_LEFT_30_TM=60.787
PRIMER_RIGHT_30_TM=58.927
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=902
PRIMER_PAIR_31_PENALTY=4.859861
PRIMER_LEFT_31_PENALTY=1.787254
PRIMER_RIGHT_31_PENALTY=3.072607
PRIMER_LEFT_31_SEQUENCE=GCCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_31_SEQUENCE=GTCTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_31=734,21
PRIMER_RIGHT_31=1637,22
PRIMER_LEFT_31_TM=60.787
PRIMER_RIGHT_31_TM=58.927
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.3000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=904
PRIMER_PAIR_32_PENALTY=5.030407
PRIMER_LEFT_32_PENALTY=4.999365
PRIMER_RIGHT_32_PENALTY=0.031043
PRIMER_LEFT_32_SEQUENCE=AGGAAAGGTTTTATTGGGAG
PRIMER_RIGHT_32_SEQUENCE=ATCCTCCAGTTGTGACCGAC
PRIMER_LEFT_32=918,20
PRIMER_RIGHT_32=1844,20
PRIMER_LEFT_32_TM=55.001
PRIMER_RIGHT_32_TM=59.969
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=927
PRIMER_PAIR_33_PENALTY=5.037076
PRIMER_LEFT_33_PENALTY=1.429676
PRIMER_RIGHT_33_PENALTY=3.607400
PRIMER_LEFT_33_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_33_SEQUENCE=TTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_33=733,21
PRIMER_RIGHT_33=1633,20
PRIMER_LEFT_33_TM=60.430
PRIMER_RIGHT_33_TM=56.393
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=901
PRIMER_PAIR_34_PENALTY=5.037076
PRIMER_LEFT_34_PENALTY=1.429676
PRIMER_RIGHT_34_PENALTY=3.607400
PRIMER_LEFT_34_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_34_SEQUENCE=TTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_34=732,21
PRIMER_RIGHT_34=1633,20
PRIMER_LEFT_34_TM=60.430
PRIMER_RIGHT_34_TM=56.393
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=902
PRIMER_PAIR_35_PENALTY=5.101405
PRIMER_LEFT_35_PENALTY=2.947383
PRIMER_RIGHT_35_PENALTY=2.154023
PRIMER_LEFT_35_SEQUENCE=CCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_35_SEQUENCE=TGTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_35=735,20
PRIMER_RIGHT_35=1631,22
PRIMER_LEFT_35_TM=57.053
PRIMER_RIGHT_35_TM=60.154
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.3000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=897
PRIMER_PAIR_36_PENALTY=5.108888
PRIMER_LEFT_36_PENALTY=4.215367
PRIMER_RIGHT_36_PENALTY=0.893521
PRIMER_LEFT_36_SEQUENCE=GGAAAGGTTTTATTGGGAGA
PRIMER_RIGHT_36_SEQUENCE=GCGATCATCAGAGCTCATCC
PRIMER_LEFT_36=919,20
PRIMER_RIGHT_36=1895,20
PRIMER_LEFT_36_TM=55.785
PRIMER_RIGHT_36_TM=60.894
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=8.1000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=977
PRIMER_PAIR_37_PENALTY=5.111157
PRIMER_LEFT_37_PENALTY=1.429676
PRIMER_RIGHT_37_PENALTY=3.681481
PRIMER_LEFT_37_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_37_SEQUENCE=TTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_37=733,21
PRIMER_RIGHT_37=1629,20
PRIMER_LEFT_37_TM=60.430
PRIMER_RIGHT_37_TM=56.319
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=897
PRIMER_PAIR_38_PENALTY=5.111157
PRIMER_LEFT_38_PENALTY=1.429676
PRIMER_RIGHT_38_PENALTY=3.681481
PRIMER_LEFT_38_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_38_SEQUENCE=TTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_38=732,21
PRIMER_RIGHT_38=1629,20
PRIMER_LEFT_38_TM=60.430
PRIMER_RIGHT_38_TM=56.319
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=898
PRIMER_PAIR_39_PENALTY=5.118588
PRIMER_LEFT_39_PENALTY=1.429676
PRIMER_RIGHT_39_PENALTY=3.688912
PRIMER_LEFT_39_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_39_SEQUENCE=GTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_39=733,21
PRIMER_RIGHT_39=1630,21
PRIMER_LEFT_39_TM=60.430
PRIMER_RIGHT_39_TM=57.311
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=898
PRIMER_PAIR_40_PENALTY=5.118588
PRIMER_LEFT_40_PENALTY=1.429676
PRIMER_RIGHT_40_PENALTY=3.688912
PRIMER_LEFT_40_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_40_SEQUENCE=GTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_40=732,21
PRIMER_RIGHT_40=1630,21
PRIMER_LEFT_40_TM=60.430
PRIMER_RIGHT_40_TM=57.311
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=899
PRIMER_PAIR_41_PENALTY=5.174498
PRIMER_LEFT_41_PENALTY=2.501813
PRIMER_RIGHT_41_PENALTY=2.672685
PRIMER_LEFT_41_SEQUENCE=CCTTTGAGTGAGCACCTTTTT
PRIMER_RIGHT_41_SEQUENCE=CGCAATTCTATCAAAATCCACC
PRIMER_LEFT_41=735,21
PRIMER_RIGHT_41=1727,22
PRIMER_LEFT_41_TM=58.498
PRIMER_RIGHT_41_TM=60.673
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=993
PRIMER_PAIR_42_PENALTY=5.220816
PRIMER_LEFT_42_PENALTY=5.189774
PRIMER_RIGHT_42_PENALTY=0.031043
PRIMER_LEFT_42_SEQUENCE=CTCTAGGAAAGGTTTTATTGGGAG
PRIMER_RIGHT_42_SEQUENCE=ATCCTCCAGTTGTGACCGAC
PRIMER_LEFT_42=914,24
PRIMER_RIGHT_42=1844,20
PRIMER_LEFT_42_TM=58.810
PRIMER_RIGHT_42_TM=59.969
PRIMER_LEFT_42_GC_PERCENT=41.667
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.4000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=931
PRIMER_PAIR_43_PENALTY=5.306516
PRIMER_LEFT_43_PENALTY=1.429676
PRIMER_RIGHT_43_PENALTY=3.876840
PRIMER_LEFT_43_SEQUENCE=AGCCTTTGAGTGAGCACCTTT
PRIMER_RIGHT_43_SEQUENCE=CTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_43=733,21
PRIMER_RIGHT_43=1635,20
PRIMER_LEFT_43_TM=60.430
PRIMER_RIGHT_43_TM=56.123
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=903
PRIMER_PAIR_44_PENALTY=5.306516
PRIMER_LEFT_44_PENALTY=1.429676
PRIMER_RIGHT_44_PENALTY=3.876840
PRIMER_LEFT_44_SEQUENCE=AAGCCTTTGAGTGAGCACCTT
PRIMER_RIGHT_44_SEQUENCE=CTTTTGTTTTGTTGCTCTGG
PRIMER_LEFT_44=732,21
PRIMER_RIGHT_44=1635,20
PRIMER_LEFT_44_TM=60.430
PRIMER_RIGHT_44_TM=56.123
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=904
PRIMER_PAIR_45_PENALTY=5.363521
PRIMER_LEFT_45_PENALTY=5.332478
PRIMER_RIGHT_45_PENALTY=0.031043
PRIMER_LEFT_45_SEQUENCE=CTCTAGGAAAGGTTTTATTGGGAGA
PRIMER_RIGHT_45_SEQUENCE=ATCCTCCAGTTGTGACCGAC
PRIMER_LEFT_45=914,25
PRIMER_RIGHT_45=1844,20
PRIMER_LEFT_45_TM=60.332
PRIMER_RIGHT_45_TM=59.969
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=9.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=931
PRIMER_PAIR_46_PENALTY=5.394655
PRIMER_LEFT_46_PENALTY=1.787254
PRIMER_RIGHT_46_PENALTY=3.607400
PRIMER_LEFT_46_SEQUENCE=GCCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_46_SEQUENCE=TTTGTTTTGTTGCTCTGGTC
PRIMER_LEFT_46=734,21
PRIMER_RIGHT_46=1633,20
PRIMER_LEFT_46_TM=60.787
PRIMER_RIGHT_46_TM=56.393
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=900
PRIMER_PAIR_47_PENALTY=5.454912
PRIMER_LEFT_47_PENALTY=4.999365
PRIMER_RIGHT_47_PENALTY=0.455547
PRIMER_LEFT_47_SEQUENCE=AGGAAAGGTTTTATTGGGAG
PRIMER_RIGHT_47_SEQUENCE=GAGGAGGAGGACATCCGTTT
PRIMER_LEFT_47=918,20
PRIMER_RIGHT_47=1865,20
PRIMER_LEFT_47_TM=55.001
PRIMER_RIGHT_47_TM=60.456
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=8.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=948
PRIMER_PAIR_48_PENALTY=5.468735
PRIMER_LEFT_48_PENALTY=1.787254
PRIMER_RIGHT_48_PENALTY=3.681481
PRIMER_LEFT_48_SEQUENCE=GCCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_48_SEQUENCE=TTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_48=734,21
PRIMER_RIGHT_48=1629,20
PRIMER_LEFT_48_TM=60.787
PRIMER_RIGHT_48_TM=56.319
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=896
PRIMER_PAIR_49_PENALTY=5.476167
PRIMER_LEFT_49_PENALTY=1.787254
PRIMER_RIGHT_49_PENALTY=3.688912
PRIMER_LEFT_49_SEQUENCE=GCCTTTGAGTGAGCACCTTTT
PRIMER_RIGHT_49_SEQUENCE=GTTTTGTTGCTCTGGTCAATC
PRIMER_LEFT_49=734,21
PRIMER_RIGHT_49=1630,21
PRIMER_LEFT_49_TM=60.787
PRIMER_RIGHT_49_TM=57.311
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=897
=
SEQUENCE_ID=MAGIC.149_2_5330804_5331445_oy-0_tsu-0_619930_8462_TRUE
SEQUENCE_TARGET=950,741
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTAACATCCCNGATCCNAANTAGGATCNAGGGGAAGGGCTGGNACNNGACTTGTAGNGANTCGGGTGAGAAAACTCGTCCCAAGNNCNGAAGAANGGNGNCNAATCGGGNAAAACANGAANGNCCAAGAGCCNGAGGGAAAGAAGATGGANTGAATAGCTCNGGAAGCTAAGTNGGAANCTANGGCAGNNNNNNNNNNNNNNNNNNNNNNNGGGGAAGCTCGGNNANCTCAAAGGGAAGCTCGNCCAGCTCNCNGGTAAGCTCGNCCAGCTCGGGANACTNNTAGCTAGNTCCNCNANCTNGACCTAGCTGGATGGGNAGTNNNNNNNNNNNTCGGCNGTTACCCGGACGGTTNCCGAANNGTTNNCCCNNCAGNTACCTTNANNNNNNNNNNNGCGGTTACCGNGCNATAAGGANGCNCCACGTGTGTGGTTGGGAAGGAAGNGCNNTGAAGGATCCNGAAGGACCTAGAGGCGCCCGTTCGNAAGNNCGGTTACTCGCNNCTTCCTTANNAATAANTNGCCCTTCGGCCAAGGAANGNACACAANTCCATACACCNAAACTCTCACNAAACTCANAAANNAAACGTGATNTTTCNAAGGGTGCNAGNNAACGGAACAANATGGNCGAGTTNGNGNCCATGGGCACTNNCGNNNGCNTTGGGCNNACNGANGCNGAGGAAGCGAANNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_5313817_5314190_mt-0_no-0_546318_435571_TRUE
SEQUENCE_TARGET=950,473
SEQUENCE_TEMPLATE=ACTAATGTTATCGTACNTGACTCATAANTTTGAGTTTTTCTTTTGAGTTAATTTTTCCAAATGGTGATAACTTTGACATTTTATTTCGTAATGTGTATAATACANCNACCGATNTTCNNTTTTTTNNNAAAGAATANTACTTACACTAACCATTTGATTTGGTTTAGTCATAATCTCTTCNTATTTCAGCCNTNTGGTCGTCGTCCGTTATATNGANNTTTTCTTATTCTATCGTTTTAACATTGTAATTACCGATTATNNTTTTTGTTGAATAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTAAANTCTAAANAAAAAANATATTTACTAANGNTTTTTGTGAAAGCAATACTAATTGCATTAACATTCATACATGATTTGTATANTTGTTTGGAGTTGGGTAAGATGCTCGACTTCCACATTAGGGAACAAACAAAGCTAGATTAANGCACGTCTAGTGAAAGATCCATCCCTNTGATTACTTAACTTCACATTAGGGAANAAACAAAGCTAGGTTCATGCACTTCTAGTGAATGATCCATCCCTTTGATTACTTAACTTCACATTTAGGGAACAAACAAAGCTAGATTCATGCACTTCTAGGTGAAGGATATCAGGATNTGCCACATTTCTATCAAAGAATACGGTTAAGAAAGTCTATAGGANGACAAAAATAGATATGTTTTCCCAAAAAAGATTCAAAGCTCAAGTTAAATGGAACAATGAGTTAGCAAAAAATGGTTTAACGGGAGGAGAAATAGTGGTAGTGTCAACCAAGTCGCCTTGGTCCAGTAGTAAAGAGAGGTAATTCCTGTCACATGGGTTCGAGTAGCTTTGGCCNCTGGNATTCGAGTAATTTAACATGNGTTTTTTCGGGTTCCAGAATTAGTCTTTGGNCCTAGAAACCCTTTAANATNNCCTGAATTATCNANANATATATATATAGTAGTAGTGTTGTGTCCTATGTTGAAAAGTAATATGGATCAGCTTTGGAATTCTCCAGGAAGCAATGGGATCATGTGGNATCAAAAAGAATAATAAAGAGACCACGATTGGGCAATTACTAGGCACTTATGTCATTGAGGTGATTAGTGGATCATAAGATTATGCCTACTGTTATCCTCAATTTGACCNTATACTNTTTTCTTCTCTTTTAGCCNTCCTTNNATACCTAACCGAGGTTCAAGAATCAGTTTATGNTTTNANGTTGGGTTGACCTTTGTTAAATAGGTTATCTAGTNANGAAGAGCGAATNAAGCCAAANTNNTTNTTTTTTTNNTNTTTAANTAAAATAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTANACACGTAGCAAGACCTAAACAGTTTTAATCTGATATAGCGNAAACTAATTTTGGCACCTTAGACAATTGATTGGTCAATTCAACNNTCAATAAGCCNAAGTGGAAAGACAAANAAATTTAGGGTATTTTGTCGAATGAGACATAATCAACTTGACTNCTCNAATAAACTGTTTTCACTAGATNATGGNTANTANTTCTNTTCTAAACATGGTTAATAGAGATCCAAAACNCAATTTNTNTATGGACTTTATTGGTGCAACTCTTTAAGTGANANTGAAACCTTACTTCAACACATTTCAAATNTATCTACCTAATTAACTCTTTATATNAAATNNANNAAAANCTTAGTGCATNAGTGGGTAGGTGANCTTNAGTCTCNTGGCAATATGTAAAGAGGCGAAATCAAAGTTGATCAACAATTAAANACAAAAACCCTTAAGTTGACGTGGCTGTATCTCTTCCAACTCTTNAAANTNAANTACTATTATACCCCTNANNAAAATGGCAGAGCGTGTGNCTCTCACACTCAACGGAACCCTCCTTNCTCCTCCTCCNNNAACAACAACAACAACAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.507366
PRIMER_LEFT_0_PENALTY=0.459180
PRIMER_RIGHT_0_PENALTY=3.048185
PRIMER_LEFT_0_SEQUENCE=TGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_0_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_0=825,20
PRIMER_RIGHT_0=1822,22
PRIMER_LEFT_0_TM=59.541
PRIMER_RIGHT_0_TM=58.952
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=998
PRIMER_PAIR_1_PENALTY=3.677996
PRIMER_LEFT_1_PENALTY=0.459180
PRIMER_RIGHT_1_PENALTY=3.218816
PRIMER_LEFT_1_SEQUENCE=TGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_1_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_1=825,20
PRIMER_RIGHT_1=1750,20
PRIMER_LEFT_1_TM=59.541
PRIMER_RIGHT_1_TM=56.781
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=926
PRIMER_PAIR_2_PENALTY=3.695553
PRIMER_LEFT_2_PENALTY=0.647368
PRIMER_RIGHT_2_PENALTY=3.048185
PRIMER_LEFT_2_SEQUENCE=AAGTCGCCTTGGTCCAGTAG
PRIMER_RIGHT_2_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_2=862,20
PRIMER_RIGHT_2=1822,22
PRIMER_LEFT_2_TM=59.353
PRIMER_RIGHT_2_TM=58.952
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=961
PRIMER_PAIR_3_PENALTY=3.853661
PRIMER_LEFT_3_PENALTY=0.805475
PRIMER_RIGHT_3_PENALTY=3.048185
PRIMER_LEFT_3_SEQUENCE=ATGGGTTCGAGTAGCTTTGG
PRIMER_RIGHT_3_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_3=905,20
PRIMER_RIGHT_3=1822,22
PRIMER_LEFT_3_TM=59.195
PRIMER_RIGHT_3_TM=58.952
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=918
PRIMER_PAIR_4_PENALTY=4.035574
PRIMER_LEFT_4_PENALTY=0.987388
PRIMER_RIGHT_4_PENALTY=3.048185
PRIMER_LEFT_4_SEQUENCE=CCTGTCACATGGGTTCGAGT
PRIMER_RIGHT_4_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_4=897,20
PRIMER_RIGHT_4=1822,22
PRIMER_LEFT_4_TM=60.987
PRIMER_RIGHT_4_TM=58.952
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=926
PRIMER_PAIR_5_PENALTY=4.123370
PRIMER_LEFT_5_PENALTY=0.459180
PRIMER_RIGHT_5_PENALTY=3.664190
PRIMER_LEFT_5_SEQUENCE=TGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_5_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_5=825,20
PRIMER_RIGHT_5=1749,20
PRIMER_LEFT_5_TM=59.541
PRIMER_RIGHT_5_TM=56.336
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=925
PRIMER_PAIR_6_PENALTY=4.206204
PRIMER_LEFT_6_PENALTY=0.987388
PRIMER_RIGHT_6_PENALTY=3.218816
PRIMER_LEFT_6_SEQUENCE=CCTGTCACATGGGTTCGAGT
PRIMER_RIGHT_6_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_6=897,20
PRIMER_RIGHT_6=1750,20
PRIMER_LEFT_6_TM=60.987
PRIMER_RIGHT_6_TM=56.781
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=10.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=854
PRIMER_PAIR_7_PENALTY=4.226343
PRIMER_LEFT_7_PENALTY=1.178157
PRIMER_RIGHT_7_PENALTY=3.048185
PRIMER_LEFT_7_SEQUENCE=ATGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_7_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_7=824,21
PRIMER_RIGHT_7=1822,22
PRIMER_LEFT_7_TM=59.822
PRIMER_RIGHT_7_TM=58.952
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=999
PRIMER_PAIR_8_PENALTY=4.226343
PRIMER_LEFT_8_PENALTY=1.178157
PRIMER_RIGHT_8_PENALTY=3.048185
PRIMER_LEFT_8_SEQUENCE=AATGGTTTAACGGGAGGAGAA
PRIMER_RIGHT_8_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_8=823,21
PRIMER_RIGHT_8=1822,22
PRIMER_LEFT_8_TM=59.822
PRIMER_RIGHT_8_TM=58.952
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=1000
PRIMER_PAIR_9_PENALTY=4.293501
PRIMER_LEFT_9_PENALTY=1.245316
PRIMER_RIGHT_9_PENALTY=3.048185
PRIMER_LEFT_9_SEQUENCE=TCGCCTTGGTCCAGTAGTAAA
PRIMER_RIGHT_9_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_9=865,21
PRIMER_RIGHT_9=1822,22
PRIMER_LEFT_9_TM=59.755
PRIMER_RIGHT_9_TM=58.952
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.0000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=958
PRIMER_PAIR_10_PENALTY=4.311557
PRIMER_LEFT_10_PENALTY=0.647368
PRIMER_RIGHT_10_PENALTY=3.664190
PRIMER_LEFT_10_SEQUENCE=AAGTCGCCTTGGTCCAGTAG
PRIMER_RIGHT_10_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_10=862,20
PRIMER_RIGHT_10=1749,20
PRIMER_LEFT_10_TM=59.353
PRIMER_RIGHT_10_TM=56.336
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.4000
PRIMER_RIGHT_10_END_STABILITY=8.8000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=888
PRIMER_PAIR_11_PENALTY=4.396973
PRIMER_LEFT_11_PENALTY=1.178157
PRIMER_RIGHT_11_PENALTY=3.218816
PRIMER_LEFT_11_SEQUENCE=ATGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_11_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_11=824,21
PRIMER_RIGHT_11=1750,20
PRIMER_LEFT_11_TM=59.822
PRIMER_RIGHT_11_TM=56.781
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=10.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=927
PRIMER_PAIR_12_PENALTY=4.396973
PRIMER_LEFT_12_PENALTY=1.178157
PRIMER_RIGHT_12_PENALTY=3.218816
PRIMER_LEFT_12_SEQUENCE=AATGGTTTAACGGGAGGAGAA
PRIMER_RIGHT_12_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_12=823,21
PRIMER_RIGHT_12=1750,20
PRIMER_LEFT_12_TM=59.822
PRIMER_RIGHT_12_TM=56.781
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=10.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=928
PRIMER_PAIR_13_PENALTY=4.396973
PRIMER_LEFT_13_PENALTY=1.178157
PRIMER_RIGHT_13_PENALTY=3.218816
PRIMER_LEFT_13_SEQUENCE=AAATGGTTTAACGGGAGGAGA
PRIMER_RIGHT_13_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_13=822,21
PRIMER_RIGHT_13=1750,20
PRIMER_LEFT_13_TM=59.822
PRIMER_RIGHT_13_TM=56.781
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=10.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=929
PRIMER_PAIR_14_PENALTY=4.545378
PRIMER_LEFT_14_PENALTY=1.497193
PRIMER_RIGHT_14_PENALTY=3.048185
PRIMER_LEFT_14_SEQUENCE=ATGGTTTAACGGGAGGAGAA
PRIMER_RIGHT_14_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_14=824,20
PRIMER_RIGHT_14=1822,22
PRIMER_LEFT_14_TM=58.503
PRIMER_RIGHT_14_TM=58.952
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=999
PRIMER_PAIR_15_PENALTY=4.545378
PRIMER_LEFT_15_PENALTY=1.497193
PRIMER_RIGHT_15_PENALTY=3.048185
PRIMER_LEFT_15_SEQUENCE=AATGGTTTAACGGGAGGAGA
PRIMER_RIGHT_15_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_15=823,20
PRIMER_RIGHT_15=1822,22
PRIMER_LEFT_15_TM=58.503
PRIMER_RIGHT_15_TM=58.952
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=1000
PRIMER_PAIR_16_PENALTY=4.651578
PRIMER_LEFT_16_PENALTY=0.987388
PRIMER_RIGHT_16_PENALTY=3.664190
PRIMER_LEFT_16_SEQUENCE=CCTGTCACATGGGTTCGAGT
PRIMER_RIGHT_16_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_16=897,20
PRIMER_RIGHT_16=1749,20
PRIMER_LEFT_16_TM=60.987
PRIMER_RIGHT_16_TM=56.336
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=8.8000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=853
PRIMER_PAIR_17_PENALTY=4.669638
PRIMER_LEFT_17_PENALTY=1.621453
PRIMER_RIGHT_17_PENALTY=3.048185
PRIMER_LEFT_17_SEQUENCE=TCGCCTTGGTCCAGTAGTAA
PRIMER_RIGHT_17_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_17=865,20
PRIMER_RIGHT_17=1822,22
PRIMER_LEFT_17_TM=58.379
PRIMER_RIGHT_17_TM=58.952
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.909
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=958
PRIMER_PAIR_18_PENALTY=4.716008
PRIMER_LEFT_18_PENALTY=1.497193
PRIMER_RIGHT_18_PENALTY=3.218816
PRIMER_LEFT_18_SEQUENCE=ATGGTTTAACGGGAGGAGAA
PRIMER_RIGHT_18_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_18=824,20
PRIMER_RIGHT_18=1750,20
PRIMER_LEFT_18_TM=58.503
PRIMER_RIGHT_18_TM=56.781
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=10.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=927
PRIMER_PAIR_19_PENALTY=4.716008
PRIMER_LEFT_19_PENALTY=1.497193
PRIMER_RIGHT_19_PENALTY=3.218816
PRIMER_LEFT_19_SEQUENCE=AATGGTTTAACGGGAGGAGA
PRIMER_RIGHT_19_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_19=823,20
PRIMER_RIGHT_19=1750,20
PRIMER_LEFT_19_TM=58.503
PRIMER_RIGHT_19_TM=56.781
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=10.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=928
PRIMER_PAIR_20_PENALTY=4.795979
PRIMER_LEFT_20_PENALTY=1.747794
PRIMER_RIGHT_20_PENALTY=3.048185
PRIMER_LEFT_20_SEQUENCE=CACATGGGTTCGAGTAGCTTT
PRIMER_RIGHT_20_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_20=902,21
PRIMER_RIGHT_20=1822,22
PRIMER_LEFT_20_TM=59.252
PRIMER_RIGHT_20_TM=58.952
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=921
PRIMER_PAIR_21_PENALTY=4.795979
PRIMER_LEFT_21_PENALTY=1.747794
PRIMER_RIGHT_21_PENALTY=3.048185
PRIMER_LEFT_21_SEQUENCE=GTCGCCTTGGTCCAGTAGTAA
PRIMER_RIGHT_21_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_21=864,21
PRIMER_RIGHT_21=1822,22
PRIMER_LEFT_21_TM=59.252
PRIMER_RIGHT_21_TM=58.952
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=5.7000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=959
PRIMER_PAIR_22_PENALTY=4.801695
PRIMER_LEFT_22_PENALTY=1.582879
PRIMER_RIGHT_22_PENALTY=3.218816
PRIMER_LEFT_22_SEQUENCE=AAAATGGTTTAACGGGAGGA
PRIMER_RIGHT_22_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_22=821,20
PRIMER_RIGHT_22=1750,20
PRIMER_LEFT_22_TM=58.417
PRIMER_RIGHT_22_TM=56.781
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=10.0000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=930
PRIMER_PAIR_23_PENALTY=4.840269
PRIMER_LEFT_23_PENALTY=1.621453
PRIMER_RIGHT_23_PENALTY=3.218816
PRIMER_LEFT_23_SEQUENCE=CATGGGTTCGAGTAGCTTTG
PRIMER_RIGHT_23_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_23=904,20
PRIMER_RIGHT_23=1750,20
PRIMER_LEFT_23_TM=58.379
PRIMER_RIGHT_23_TM=56.781
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=10.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=847
PRIMER_PAIR_24_PENALTY=4.840269
PRIMER_LEFT_24_PENALTY=1.621453
PRIMER_RIGHT_24_PENALTY=3.218816
PRIMER_LEFT_24_SEQUENCE=TCGCCTTGGTCCAGTAGTAA
PRIMER_RIGHT_24_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_24=865,20
PRIMER_RIGHT_24=1750,20
PRIMER_LEFT_24_TM=58.379
PRIMER_RIGHT_24_TM=56.781
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=5.7000
PRIMER_RIGHT_24_END_STABILITY=10.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=886
PRIMER_PAIR_25_PENALTY=4.842347
PRIMER_LEFT_25_PENALTY=1.178157
PRIMER_RIGHT_25_PENALTY=3.664190
PRIMER_LEFT_25_SEQUENCE=ATGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_25_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_25=824,21
PRIMER_RIGHT_25=1749,20
PRIMER_LEFT_25_TM=59.822
PRIMER_RIGHT_25_TM=56.336
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.8000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=926
PRIMER_PAIR_26_PENALTY=4.842347
PRIMER_LEFT_26_PENALTY=1.178157
PRIMER_RIGHT_26_PENALTY=3.664190
PRIMER_LEFT_26_SEQUENCE=AATGGTTTAACGGGAGGAGAA
PRIMER_RIGHT_26_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_26=823,21
PRIMER_RIGHT_26=1749,20
PRIMER_LEFT_26_TM=59.822
PRIMER_RIGHT_26_TM=56.336
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=927
PRIMER_PAIR_27_PENALTY=4.842347
PRIMER_LEFT_27_PENALTY=1.178157
PRIMER_RIGHT_27_PENALTY=3.664190
PRIMER_LEFT_27_SEQUENCE=AAATGGTTTAACGGGAGGAGA
PRIMER_RIGHT_27_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_27=822,21
PRIMER_RIGHT_27=1749,20
PRIMER_LEFT_27_TM=59.822
PRIMER_RIGHT_27_TM=56.336
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=8.8000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=928
PRIMER_PAIR_28_PENALTY=4.859518
PRIMER_LEFT_28_PENALTY=1.640703
PRIMER_RIGHT_28_PENALTY=3.218816
PRIMER_LEFT_28_SEQUENCE=AAAATGGTTTAACGGGAGGAG
PRIMER_RIGHT_28_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_28=821,21
PRIMER_RIGHT_28=1750,20
PRIMER_LEFT_28_TM=59.359
PRIMER_RIGHT_28_TM=56.781
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=930
PRIMER_PAIR_29_PENALTY=4.909505
PRIMER_LEFT_29_PENALTY=1.245316
PRIMER_RIGHT_29_PENALTY=3.664190
PRIMER_LEFT_29_SEQUENCE=TCGCCTTGGTCCAGTAGTAAA
PRIMER_RIGHT_29_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_29=865,21
PRIMER_RIGHT_29=1749,20
PRIMER_LEFT_29_TM=59.755
PRIMER_RIGHT_29_TM=56.336
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.0000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=885
PRIMER_PAIR_30_PENALTY=4.927052
PRIMER_LEFT_30_PENALTY=1.878867
PRIMER_RIGHT_30_PENALTY=3.048185
PRIMER_LEFT_30_SEQUENCE=AACGGGAGGAGAAATAGTGG
PRIMER_RIGHT_30_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_30=831,20
PRIMER_RIGHT_30=1822,22
PRIMER_LEFT_30_TM=58.121
PRIMER_RIGHT_30_TM=58.952
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=992
PRIMER_PAIR_31_PENALTY=4.966609
PRIMER_LEFT_31_PENALTY=1.747794
PRIMER_RIGHT_31_PENALTY=3.218816
PRIMER_LEFT_31_SEQUENCE=ACATGGGTTCGAGTAGCTTTG
PRIMER_RIGHT_31_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_31=903,21
PRIMER_RIGHT_31=1750,20
PRIMER_LEFT_31_TM=59.252
PRIMER_RIGHT_31_TM=56.781
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.3000
PRIMER_RIGHT_31_END_STABILITY=10.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=848
PRIMER_PAIR_32_PENALTY=4.966609
PRIMER_LEFT_32_PENALTY=1.747794
PRIMER_RIGHT_32_PENALTY=3.218816
PRIMER_LEFT_32_SEQUENCE=CACATGGGTTCGAGTAGCTTT
PRIMER_RIGHT_32_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_32=902,21
PRIMER_RIGHT_32=1750,20
PRIMER_LEFT_32_TM=59.252
PRIMER_RIGHT_32_TM=56.781
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=10.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=849
PRIMER_PAIR_33_PENALTY=4.966609
PRIMER_LEFT_33_PENALTY=1.747794
PRIMER_RIGHT_33_PENALTY=3.218816
PRIMER_LEFT_33_SEQUENCE=GTCGCCTTGGTCCAGTAGTAA
PRIMER_RIGHT_33_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_33=864,21
PRIMER_RIGHT_33=1750,20
PRIMER_LEFT_33_TM=59.252
PRIMER_RIGHT_33_TM=56.781
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=5.7000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=887
PRIMER_PAIR_34_PENALTY=5.070619
PRIMER_LEFT_34_PENALTY=2.022434
PRIMER_RIGHT_34_PENALTY=3.048185
PRIMER_LEFT_34_SEQUENCE=AACGGGAGGAGAAATAGTGGT
PRIMER_RIGHT_34_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_34=831,21
PRIMER_RIGHT_34=1822,22
PRIMER_LEFT_34_TM=58.978
PRIMER_RIGHT_34_TM=58.952
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.6000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=992
PRIMER_PAIR_35_PENALTY=5.097683
PRIMER_LEFT_35_PENALTY=1.878867
PRIMER_RIGHT_35_PENALTY=3.218816
PRIMER_LEFT_35_SEQUENCE=AACGGGAGGAGAAATAGTGG
PRIMER_RIGHT_35_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_35=831,20
PRIMER_RIGHT_35=1750,20
PRIMER_LEFT_35_TM=58.121
PRIMER_RIGHT_35_TM=56.781
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=10.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=920
PRIMER_PAIR_36_PENALTY=5.134016
PRIMER_LEFT_36_PENALTY=2.085831
PRIMER_RIGHT_36_PENALTY=3.048185
PRIMER_LEFT_36_SEQUENCE=CGCCTTGGTCCAGTAGTAAAG
PRIMER_RIGHT_36_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_36=866,21
PRIMER_RIGHT_36=1822,22
PRIMER_LEFT_36_TM=58.914
PRIMER_RIGHT_36_TM=58.952
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.3000
PRIMER_RIGHT_36_END_STABILITY=8.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=957
PRIMER_PAIR_37_PENALTY=5.141249
PRIMER_LEFT_37_PENALTY=2.093064
PRIMER_RIGHT_37_PENALTY=3.048185
PRIMER_LEFT_37_SEQUENCE=CGCCTTGGTCCAGTAGTAAA
PRIMER_RIGHT_37_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_37=866,20
PRIMER_RIGHT_37=1822,22
PRIMER_LEFT_37_TM=57.907
PRIMER_RIGHT_37_TM=58.952
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.0000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=957
PRIMER_PAIR_38_PENALTY=5.161382
PRIMER_LEFT_38_PENALTY=1.497193
PRIMER_RIGHT_38_PENALTY=3.664190
PRIMER_LEFT_38_SEQUENCE=ATGGTTTAACGGGAGGAGAA
PRIMER_RIGHT_38_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_38=824,20
PRIMER_RIGHT_38=1749,20
PRIMER_LEFT_38_TM=58.503
PRIMER_RIGHT_38_TM=56.336
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=8.8000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=926
PRIMER_PAIR_39_PENALTY=5.161382
PRIMER_LEFT_39_PENALTY=1.497193
PRIMER_RIGHT_39_PENALTY=3.664190
PRIMER_LEFT_39_SEQUENCE=AATGGTTTAACGGGAGGAGA
PRIMER_RIGHT_39_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_39=823,20
PRIMER_RIGHT_39=1749,20
PRIMER_LEFT_39_TM=58.503
PRIMER_RIGHT_39_TM=56.336
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=8.8000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=927
PRIMER_PAIR_40_PENALTY=5.172553
PRIMER_LEFT_40_PENALTY=1.953737
PRIMER_RIGHT_40_PENALTY=3.218816
PRIMER_LEFT_40_SEQUENCE=AAATGGTTTAACGGGAGGAG
PRIMER_RIGHT_40_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_40=822,20
PRIMER_RIGHT_40=1750,20
PRIMER_LEFT_40_TM=58.046
PRIMER_RIGHT_40_TM=56.781
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=10.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=929
PRIMER_PAIR_41_PENALTY=5.226614
PRIMER_LEFT_41_PENALTY=0.459180
PRIMER_RIGHT_41_PENALTY=4.767434
PRIMER_LEFT_41_SEQUENCE=TGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_41_SEQUENCE=GTCTCATTCGACAAAATACCCT
PRIMER_LEFT_41=825,20
PRIMER_RIGHT_41=1821,22
PRIMER_LEFT_41_TM=59.541
PRIMER_RIGHT_41_TM=57.233
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=9.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=997
PRIMER_PAIR_42_PENALTY=5.233241
PRIMER_LEFT_42_PENALTY=2.185055
PRIMER_RIGHT_42_PENALTY=3.048185
PRIMER_LEFT_42_SEQUENCE=AGTCGCCTTGGTCCAGTAGTAA
PRIMER_RIGHT_42_SEQUENCE=TGTCTCATTCGACAAAATACCC
PRIMER_LEFT_42=863,22
PRIMER_RIGHT_42=1822,22
PRIMER_LEFT_42_TM=60.185
PRIMER_RIGHT_42_TM=58.952
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.7000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=960
PRIMER_PAIR_43_PENALTY=5.241250
PRIMER_LEFT_43_PENALTY=2.022434
PRIMER_RIGHT_43_PENALTY=3.218816
PRIMER_LEFT_43_SEQUENCE=AACGGGAGGAGAAATAGTGGT
PRIMER_RIGHT_43_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_43=831,21
PRIMER_RIGHT_43=1750,20
PRIMER_LEFT_43_TM=58.978
PRIMER_RIGHT_43_TM=56.781
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=10.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=920
PRIMER_PAIR_44_PENALTY=5.243074
PRIMER_LEFT_44_PENALTY=2.024258
PRIMER_RIGHT_44_PENALTY=3.218816
PRIMER_LEFT_44_SEQUENCE=AAAAATGGTTTAACGGGAGG
PRIMER_RIGHT_44_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_44=820,20
PRIMER_RIGHT_44=1750,20
PRIMER_LEFT_44_TM=57.976
PRIMER_RIGHT_44_TM=56.781
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=10.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=931
PRIMER_PAIR_45_PENALTY=5.247069
PRIMER_LEFT_45_PENALTY=1.582879
PRIMER_RIGHT_45_PENALTY=3.664190
PRIMER_LEFT_45_SEQUENCE=AAAATGGTTTAACGGGAGGA
PRIMER_RIGHT_45_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_45=821,20
PRIMER_RIGHT_45=1749,20
PRIMER_LEFT_45_TM=58.417
PRIMER_RIGHT_45_TM=56.336
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=929
PRIMER_PAIR_46_PENALTY=5.285643
PRIMER_LEFT_46_PENALTY=1.621453
PRIMER_RIGHT_46_PENALTY=3.664190
PRIMER_LEFT_46_SEQUENCE=TCGCCTTGGTCCAGTAGTAA
PRIMER_RIGHT_46_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_46=865,20
PRIMER_RIGHT_46=1749,20
PRIMER_LEFT_46_TM=58.379
PRIMER_RIGHT_46_TM=56.336
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=5.7000
PRIMER_RIGHT_46_END_STABILITY=8.8000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=885
PRIMER_PAIR_47_PENALTY=5.304647
PRIMER_LEFT_47_PENALTY=2.085831
PRIMER_RIGHT_47_PENALTY=3.218816
PRIMER_LEFT_47_SEQUENCE=CGCCTTGGTCCAGTAGTAAAG
PRIMER_RIGHT_47_SEQUENCE=TCAATTGTCTAAGGTGCCAA
PRIMER_LEFT_47=866,21
PRIMER_RIGHT_47=1750,20
PRIMER_LEFT_47_TM=58.914
PRIMER_RIGHT_47_TM=56.781
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.3000
PRIMER_RIGHT_47_END_STABILITY=10.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=885
PRIMER_PAIR_48_PENALTY=5.304892
PRIMER_LEFT_48_PENALTY=1.640703
PRIMER_RIGHT_48_PENALTY=3.664190
PRIMER_LEFT_48_SEQUENCE=AAAATGGTTTAACGGGAGGAG
PRIMER_RIGHT_48_SEQUENCE=CAATTGTCTAAGGTGCCAAA
PRIMER_LEFT_48=821,21
PRIMER_RIGHT_48=1749,20
PRIMER_LEFT_48_TM=59.359
PRIMER_RIGHT_48_TM=56.336
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=8.8000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=929
PRIMER_PAIR_49_PENALTY=5.311990
PRIMER_LEFT_49_PENALTY=0.459180
PRIMER_RIGHT_49_PENALTY=4.852810
PRIMER_LEFT_49_SEQUENCE=TGGTTTAACGGGAGGAGAAA
PRIMER_RIGHT_49_SEQUENCE=GTCTCATTCGACAAAATACCC
PRIMER_LEFT_49=825,20
PRIMER_RIGHT_49=1821,21
PRIMER_LEFT_49_TM=59.541
PRIMER_RIGHT_49_TM=56.147
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=997
=
SEQUENCE_ID=MAGIC.287_4_4626751_4627051_tsu-0_po-0_15818_88054_TRUE
SEQUENCE_TARGET=950,400
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_15069509_15070046_zu-0_edi-0_63324_610197_TRUE
SEQUENCE_TARGET=950,637
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4739737_4739835_ler-0_zu-0_11293_4784_TRUE
SEQUENCE_TARGET=950,198
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_13684576_13685305_wil-2_ct-1_71471_506466_TRUE
SEQUENCE_TARGET=950,829
SEQUENCE_TEMPLATE=AANGATCNTTTNTTTTNTNNNTNNANATAGGANATTCATGTCTTGATTCAAATANTTTGNTATTCANTTTGAGGTGAACGAAAAANCNANNNNNNNNNNNNNNNTTTTATTTATAATAGAAAANNNNTCGACAATANCATAGGANAGGAAGCTGNTTATGTNTAATNNNTANGATATGANAAACTTCGAAANTAAGNNAAATAANNNNNNNNNNNNNNNNNATTAGCATGTCGAGCAGTGAATTCTCGNGCTCTCTGGTCAAATTGGAATCTGTTATTCTTGAAGAGCTGTCCAATNTCAGGTACAAGAGGATCATCTGGNTTTGGTTTTTCCAANAGTAAAANTATTGACAGAAGCACCTGTTTGTAAAATTANTTANGAACCAAAATTAATACNTAAATGCCTNAANTAATTCNAAAAACAATAAACAAAATATCTGAATCGAGTTTGAAANCTTTTCAACNNTAAGAGNAGGAGTCCANNTGTCTTTAAGAATGTNNATGCAAATNGATCCTTCATCATTGATATTTNGATGGTAAATCGGAGTTNTNAANGTAANCTGTAATANATNNANNAACATATTTACTTTTATAAACAACAAAATCTTTAGGAAANNATNNNNNNNNNNNNNNNNNNATAGCATATNTNTATAACCAACCTTGGGTGGTTTNAAAGGATAATCNGTAGGAAACTTAATACTGANATTAAACATGCCACCTTCATAGGGAGTNCCANNNNNNNNNNNNNNNNNNNNNNTCCATTNGTATATATNGTNCGAGTTNNNACCTNTANAAAANACATTTCATCANNAAATAGTATTGTTATANTTNANACAAAACAAATAAAANGTTAAAATAATTTNATTAGTTTGGTTATTGTTACAAATGAAAATATTAATAAGGTTTCANACANTAGATAGCCTNTTATTTTAAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTNTTTTAAANAATAGCANGTTATTTAAAAACAANNCNCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAACTAAATTACCCCTAAACTTAATGATCAAAATTTGGATCCTACAAAAACTCAACCGTAGATGTAATCAGAAAAGTCTCCTCGTTTAATAGCTTGCACTTGGCATGATTTCTTTAACTTTCTTGGTCATTAGTCATT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_5792963_5793265_kn-0_po-0_15269_255030_TRUE
SEQUENCE_TARGET=950,402
SEQUENCE_TEMPLATE=TATGAAACAAAATTNNATTTGTCTTTATGTTGCTTNCATATAGAAAAGTNCAAAACTTTGTTAGAGCTCATGTAACTTCNTNNNNNNNNAAAACAAAACAAATCAGATCCAAAGTGTAATAAAGAGAAACTCTAGGGACTTCTTTATAAATAAAACAANAANCATTTACACGTCTTCGTGTAATTTCGAAGAAACATCNATTCTCTTCTCTTATATAAACCCTCTTCGTCTCCTCCTCCTCTCTGCTCGAAACAAGATCCTTCACTTCTCTCAAACTACGAAAACTATGGCTTCAAAATCCNTCGTCGTTTTNCTCTTCCTCNNTCTCATAGCTTCNTCAGCCATAGCTCAAGCTCCAGGACCAGCNCCAACAAGATCTCCTCTTCCATCTCCTGCTCAACCTCCAAGAACCGCCGCACCAACTCCTTCAATCACACCAACNCCAACTCCAACACCATCCGCTACTCCCACGGCGGCTCCNGTTTCTCCTCCGGCTGGTTCACCACTTCCCTCNTCCGCTNCACCACCCGCTCCTCCAACTTCTCTCACTCCNGATGGTGCNCCTGTCGCTGGTCCGACTGGATCNACTCCCGTCGANAACAACAACGCCGCCACTCTCGCNGCTGGATCTTTAGCCGGATTTGTATTCGTCGCTTCTTTGTTACTGTAATTTGGTTATATTTTAGAATCATCTATTGAGATAGNGTTTTTCTATTTAAGGATTTTTTTGTTCGTGTTATTTTTAATCTTTGGTTTTATGTTGTATTAAGAATTGAGTTAGCGNTTGGACGATAGAATTAAANCANTAATCNNNTGTNTTTTNTATTATAGTCTTTAGTAAACANNNNNNNGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTTATCACTAATGTTTTATTATGGTATTNNANANTNCGTATGGATTTGAATTAATTGATTTAGTGAAGCCTTGTGGAATTCGAAATGGGTGGGGTTAAGTGGTTAGTGAAATAGAAGGTGATTAGTGATGACTGATGAGGTCAGCAAACGTGTGCGTGTGTAAAGACACGTGTCGACNTATATAAGAACGGTGAATTTGTCGTTTTATTGGTAGTGGCGAAAAAACAGCTGTTTGGTTTTCTGTCTGATTCTTCATCTCTTTTTTTTACACTTTTATTTGAGCTAAACGATNNAAATGAGATTGGATAACGAAGATTGAAAAGTATGTAATATTAACGAGACTAATGGCGTTTAGAATTGAAAAATCGANTTAAAAAAGTCTTTGGTTTGGTGAGAAAGGTAAGAGTAGGTTTTGTCACGTGGAGACAGCTGTAAGAATACATATAAAGCNTTTTTCAGGATTCTTTCATTGGCTCAGCCATGTGGGCTTTCCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTAAAGGTGGCGTGACAAAATATTATACTCAATACTATAAGTTACTTAAAATTANGAAAAANTTTAATTAAATCAATGCGTTTGCTATAAAAGTTAGANGAAGAAGATATACTATCGTTTTTTTGTTAACATAAATCGAAAAGAACTCTCAAGTCTTTTTAGGTTTTTGCACAAAAGGAGGATAAAGAGAAAAAAAGGTAAAAAGAAAGTTGAAGAAATTGTTCCNTTTTAAAGGGAAAGGCTGGAGAGCATTTGCATTCNTTAGCTGTGAATTCTGATTCATTTTCATTCCCTCCTTAATATTCCAACTCAACGAAAATGAAAAGTANCCACAATTTGTCCTTTTTCATATACCAATGTCATTTATTCTTTAGCCATTAATAACCTNTAAAANGTACCACATAAAATTTTATANATAAAGAAAAAGNATGGAAGACAAAGTACAAGAAGCCTTTTTTGAATTAGTACTTTAGTACCAAATGCTCGAGGTGANATCTAACTGAACCATAGTGTTGTTGCCNCGCGAAAAGGANAANGTCTCAAGCAAGAAATCTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.432188
PRIMER_LEFT_0_PENALTY=0.003322
PRIMER_RIGHT_0_PENALTY=0.428866
PRIMER_LEFT_0_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_0_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_0=445,20
PRIMER_RIGHT_0=1438,20
PRIMER_LEFT_0_TM=59.997
PRIMER_RIGHT_0_TM=59.571
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=994
PRIMER_PAIR_1_PENALTY=0.524494
PRIMER_LEFT_1_PENALTY=0.095629
PRIMER_RIGHT_1_PENALTY=0.428866
PRIMER_LEFT_1_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_1_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_1=636,20
PRIMER_RIGHT_1=1438,20
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.571
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=803
PRIMER_PAIR_2_PENALTY=0.562606
PRIMER_LEFT_2_PENALTY=0.133741
PRIMER_RIGHT_2_PENALTY=0.428866
PRIMER_LEFT_2_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_2_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_2=446,20
PRIMER_RIGHT_2=1438,20
PRIMER_LEFT_2_TM=60.134
PRIMER_RIGHT_2_TM=59.571
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=993
PRIMER_PAIR_3_PENALTY=1.096931
PRIMER_LEFT_3_PENALTY=0.668065
PRIMER_RIGHT_3_PENALTY=0.428866
PRIMER_LEFT_3_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_3_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_3=528,20
PRIMER_RIGHT_3=1438,20
PRIMER_LEFT_3_TM=60.668
PRIMER_RIGHT_3_TM=59.571
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=911
PRIMER_PAIR_4_PENALTY=1.495435
PRIMER_LEFT_4_PENALTY=1.066570
PRIMER_RIGHT_4_PENALTY=0.428866
PRIMER_LEFT_4_SEQUENCE=TCCAACACCATCCGCTACTC
PRIMER_RIGHT_4_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_4=447,20
PRIMER_RIGHT_4=1438,20
PRIMER_LEFT_4_TM=61.067
PRIMER_RIGHT_4_TM=59.571
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=992
PRIMER_PAIR_5_PENALTY=1.598310
PRIMER_LEFT_5_PENALTY=0.003322
PRIMER_RIGHT_5_PENALTY=1.594988
PRIMER_LEFT_5_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_5_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_5=445,20
PRIMER_RIGHT_5=1438,21
PRIMER_LEFT_5_TM=59.997
PRIMER_RIGHT_5_TM=60.595
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=994
PRIMER_PAIR_6_PENALTY=1.598310
PRIMER_LEFT_6_PENALTY=0.003322
PRIMER_RIGHT_6_PENALTY=1.594988
PRIMER_LEFT_6_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_6_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_6=445,20
PRIMER_RIGHT_6=1439,21
PRIMER_LEFT_6_TM=59.997
PRIMER_RIGHT_6_TM=60.595
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=995
PRIMER_PAIR_7_PENALTY=1.690616
PRIMER_LEFT_7_PENALTY=0.095629
PRIMER_RIGHT_7_PENALTY=1.594988
PRIMER_LEFT_7_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_7_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_7=636,20
PRIMER_RIGHT_7=1438,21
PRIMER_LEFT_7_TM=60.096
PRIMER_RIGHT_7_TM=60.595
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=10.2000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=803
PRIMER_PAIR_8_PENALTY=1.690616
PRIMER_LEFT_8_PENALTY=0.095629
PRIMER_RIGHT_8_PENALTY=1.594988
PRIMER_LEFT_8_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_8_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_8=636,20
PRIMER_RIGHT_8=1439,21
PRIMER_LEFT_8_TM=60.096
PRIMER_RIGHT_8_TM=60.595
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=804
PRIMER_PAIR_9_PENALTY=1.728728
PRIMER_LEFT_9_PENALTY=0.133741
PRIMER_RIGHT_9_PENALTY=1.594988
PRIMER_LEFT_9_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_9_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_9=446,20
PRIMER_RIGHT_9=1438,21
PRIMER_LEFT_9_TM=60.134
PRIMER_RIGHT_9_TM=60.595
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=993
PRIMER_PAIR_10_PENALTY=1.728728
PRIMER_LEFT_10_PENALTY=0.133741
PRIMER_RIGHT_10_PENALTY=1.594988
PRIMER_LEFT_10_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_10_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_10=446,20
PRIMER_RIGHT_10=1439,21
PRIMER_LEFT_10_TM=60.134
PRIMER_RIGHT_10_TM=60.595
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=994
PRIMER_PAIR_11_PENALTY=1.802576
PRIMER_LEFT_11_PENALTY=0.003322
PRIMER_RIGHT_11_PENALTY=1.799253
PRIMER_LEFT_11_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_11_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_11=445,20
PRIMER_RIGHT_11=1410,21
PRIMER_LEFT_11_TM=59.997
PRIMER_RIGHT_11_TM=59.201
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=966
PRIMER_PAIR_12_PENALTY=1.830375
PRIMER_LEFT_12_PENALTY=0.031122
PRIMER_RIGHT_12_PENALTY=1.799253
PRIMER_LEFT_12_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_12_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_12=624,20
PRIMER_RIGHT_12=1410,21
PRIMER_LEFT_12_TM=60.031
PRIMER_RIGHT_12_TM=59.201
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=787
PRIMER_PAIR_13_PENALTY=1.864550
PRIMER_LEFT_13_PENALTY=1.435685
PRIMER_RIGHT_13_PENALTY=0.428866
PRIMER_LEFT_13_SEQUENCE=CCGCTCCTCCAACTTCTCTC
PRIMER_RIGHT_13_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_13=527,20
PRIMER_RIGHT_13=1438,20
PRIMER_LEFT_13_TM=61.436
PRIMER_RIGHT_13_TM=59.571
PRIMER_LEFT_13_GC_PERCENT=60.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=912
PRIMER_PAIR_14_PENALTY=1.883935
PRIMER_LEFT_14_PENALTY=0.031122
PRIMER_RIGHT_14_PENALTY=1.852814
PRIMER_LEFT_14_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_14_SEQUENCE=TATTTTGTCACGCCACCTTT
PRIMER_LEFT_14=624,20
PRIMER_RIGHT_14=1556,20
PRIMER_LEFT_14_TM=60.031
PRIMER_RIGHT_14_TM=58.147
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.2000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=933
PRIMER_PAIR_15_PENALTY=1.894882
PRIMER_LEFT_15_PENALTY=0.095629
PRIMER_RIGHT_15_PENALTY=1.799253
PRIMER_LEFT_15_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_15_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_15=636,20
PRIMER_RIGHT_15=1410,21
PRIMER_LEFT_15_TM=60.096
PRIMER_RIGHT_15_TM=59.201
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.2000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=775
PRIMER_PAIR_16_PENALTY=1.932994
PRIMER_LEFT_16_PENALTY=0.133741
PRIMER_RIGHT_16_PENALTY=1.799253
PRIMER_LEFT_16_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_16_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_16=446,20
PRIMER_RIGHT_16=1410,21
PRIMER_LEFT_16_TM=60.134
PRIMER_RIGHT_16_TM=59.201
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=2.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=965
PRIMER_PAIR_17_PENALTY=1.948442
PRIMER_LEFT_17_PENALTY=0.095629
PRIMER_RIGHT_17_PENALTY=1.852814
PRIMER_LEFT_17_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_17_SEQUENCE=TATTTTGTCACGCCACCTTT
PRIMER_LEFT_17=636,20
PRIMER_RIGHT_17=1556,20
PRIMER_LEFT_17_TM=60.096
PRIMER_RIGHT_17_TM=58.147
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=10.2000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=921
PRIMER_PAIR_18_PENALTY=2.015475
PRIMER_LEFT_18_PENALTY=0.003322
PRIMER_RIGHT_18_PENALTY=2.012153
PRIMER_LEFT_18_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_18_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_18=445,20
PRIMER_RIGHT_18=1410,22
PRIMER_LEFT_18_TM=59.997
PRIMER_RIGHT_18_TM=60.012
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.455
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=2.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=966
PRIMER_PAIR_19_PENALTY=2.015475
PRIMER_LEFT_19_PENALTY=0.003322
PRIMER_RIGHT_19_PENALTY=2.012153
PRIMER_LEFT_19_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_19_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_19=445,20
PRIMER_RIGHT_19=1411,22
PRIMER_LEFT_19_TM=59.997
PRIMER_RIGHT_19_TM=60.012
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=2.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=967
PRIMER_PAIR_20_PENALTY=2.043275
PRIMER_LEFT_20_PENALTY=0.031122
PRIMER_RIGHT_20_PENALTY=2.012153
PRIMER_LEFT_20_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_20_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_20=624,20
PRIMER_RIGHT_20=1410,22
PRIMER_LEFT_20_TM=60.031
PRIMER_RIGHT_20_TM=60.012
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=787
PRIMER_PAIR_21_PENALTY=2.043275
PRIMER_LEFT_21_PENALTY=0.031122
PRIMER_RIGHT_21_PENALTY=2.012153
PRIMER_LEFT_21_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_21_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_21=624,20
PRIMER_RIGHT_21=1411,22
PRIMER_LEFT_21_TM=60.031
PRIMER_RIGHT_21_TM=60.012
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.2000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=788
PRIMER_PAIR_22_PENALTY=2.107782
PRIMER_LEFT_22_PENALTY=0.095629
PRIMER_RIGHT_22_PENALTY=2.012153
PRIMER_LEFT_22_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_22_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_22=636,20
PRIMER_RIGHT_22=1410,22
PRIMER_LEFT_22_TM=60.096
PRIMER_RIGHT_22_TM=60.012
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=10.2000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=775
PRIMER_PAIR_23_PENALTY=2.107782
PRIMER_LEFT_23_PENALTY=0.095629
PRIMER_RIGHT_23_PENALTY=2.012153
PRIMER_LEFT_23_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_23_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_23=636,20
PRIMER_RIGHT_23=1411,22
PRIMER_LEFT_23_TM=60.096
PRIMER_RIGHT_23_TM=60.012
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.2000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=776
PRIMER_PAIR_24_PENALTY=2.145894
PRIMER_LEFT_24_PENALTY=0.133741
PRIMER_RIGHT_24_PENALTY=2.012153
PRIMER_LEFT_24_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_24_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_24=446,20
PRIMER_RIGHT_24=1410,22
PRIMER_LEFT_24_TM=60.134
PRIMER_RIGHT_24_TM=60.012
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=5.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=965
PRIMER_PAIR_25_PENALTY=2.145894
PRIMER_LEFT_25_PENALTY=0.133741
PRIMER_RIGHT_25_PENALTY=2.012153
PRIMER_LEFT_25_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_25_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_25=446,20
PRIMER_RIGHT_25=1411,22
PRIMER_LEFT_25_TM=60.134
PRIMER_RIGHT_25_TM=60.012
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=5.4000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=966
PRIMER_PAIR_26_PENALTY=2.263053
PRIMER_LEFT_26_PENALTY=0.668065
PRIMER_RIGHT_26_PENALTY=1.594988
PRIMER_LEFT_26_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_26_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_26=528,20
PRIMER_RIGHT_26=1438,21
PRIMER_LEFT_26_TM=60.668
PRIMER_RIGHT_26_TM=60.595
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=911
PRIMER_PAIR_27_PENALTY=2.263053
PRIMER_LEFT_27_PENALTY=0.668065
PRIMER_RIGHT_27_PENALTY=1.594988
PRIMER_LEFT_27_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_27_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_27=528,20
PRIMER_RIGHT_27=1439,21
PRIMER_LEFT_27_TM=60.668
PRIMER_RIGHT_27_TM=60.595
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=912
PRIMER_PAIR_28_PENALTY=2.296575
PRIMER_LEFT_28_PENALTY=0.031122
PRIMER_RIGHT_28_PENALTY=2.265453
PRIMER_LEFT_28_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_28_SEQUENCE=GCCAATGAAAGAATCCTGAA
PRIMER_LEFT_28=624,20
PRIMER_RIGHT_28=1484,20
PRIMER_LEFT_28_TM=60.031
PRIMER_RIGHT_28_TM=57.735
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=861
PRIMER_PAIR_29_PENALTY=2.361082
PRIMER_LEFT_29_PENALTY=0.095629
PRIMER_RIGHT_29_PENALTY=2.265453
PRIMER_LEFT_29_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_29_SEQUENCE=GCCAATGAAAGAATCCTGAA
PRIMER_LEFT_29=636,20
PRIMER_RIGHT_29=1484,20
PRIMER_LEFT_29_TM=60.096
PRIMER_RIGHT_29_TM=57.735
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=10.2000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=849
PRIMER_PAIR_30_PENALTY=2.375857
PRIMER_LEFT_30_PENALTY=1.946991
PRIMER_RIGHT_30_PENALTY=0.428866
PRIMER_LEFT_30_SEQUENCE=ACTCCAACACCATCCGCTACT
PRIMER_RIGHT_30_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_30=445,21
PRIMER_RIGHT_30=1438,20
PRIMER_LEFT_30_TM=60.947
PRIMER_RIGHT_30_TM=59.571
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=5.4000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=994
PRIMER_PAIR_31_PENALTY=2.394367
PRIMER_LEFT_31_PENALTY=0.003322
PRIMER_RIGHT_31_PENALTY=2.391045
PRIMER_LEFT_31_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_31_SEQUENCE=GTCTCCACGTGACAAAACCT
PRIMER_LEFT_31=445,20
PRIMER_RIGHT_31=1437,20
PRIMER_LEFT_31_TM=59.997
PRIMER_RIGHT_31_TM=57.609
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=993
PRIMER_PAIR_32_PENALTY=2.453124
PRIMER_LEFT_32_PENALTY=2.024258
PRIMER_RIGHT_32_PENALTY=0.428866
PRIMER_LEFT_32_SEQUENCE=GCTGGTTCACCACTTCCCTC
PRIMER_RIGHT_32_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_32=493,20
PRIMER_RIGHT_32=1438,20
PRIMER_LEFT_32_TM=62.024
PRIMER_RIGHT_32_TM=59.571
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=946
PRIMER_PAIR_33_PENALTY=2.467318
PRIMER_LEFT_33_PENALTY=0.668065
PRIMER_RIGHT_33_PENALTY=1.799253
PRIMER_LEFT_33_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_33_SEQUENCE=CCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_33=528,20
PRIMER_RIGHT_33=1410,21
PRIMER_LEFT_33_TM=60.668
PRIMER_RIGHT_33_TM=59.201
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=883
PRIMER_PAIR_34_PENALTY=2.486673
PRIMER_LEFT_34_PENALTY=0.095629
PRIMER_RIGHT_34_PENALTY=2.391045
PRIMER_LEFT_34_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_34_SEQUENCE=GTCTCCACGTGACAAAACCT
PRIMER_LEFT_34=636,20
PRIMER_RIGHT_34=1437,20
PRIMER_LEFT_34_TM=60.096
PRIMER_RIGHT_34_TM=57.609
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.2000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=802
PRIMER_PAIR_35_PENALTY=2.524785
PRIMER_LEFT_35_PENALTY=0.133741
PRIMER_RIGHT_35_PENALTY=2.391045
PRIMER_LEFT_35_SEQUENCE=CTCCAACACCATCCGCTACT
PRIMER_RIGHT_35_SEQUENCE=GTCTCCACGTGACAAAACCT
PRIMER_LEFT_35=446,20
PRIMER_RIGHT_35=1437,20
PRIMER_LEFT_35_TM=60.134
PRIMER_RIGHT_35_TM=57.609
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=5.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=2.593204
PRIMER_LEFT_36_PENALTY=0.031122
PRIMER_RIGHT_36_PENALTY=2.562082
PRIMER_LEFT_36_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_36_SEQUENCE=GAGCCAATGAAAGAATCCTGAA
PRIMER_LEFT_36=624,20
PRIMER_RIGHT_36=1486,22
PRIMER_LEFT_36_TM=60.031
PRIMER_RIGHT_36_TM=60.562
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.2000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=863
PRIMER_PAIR_37_PENALTY=2.657711
PRIMER_LEFT_37_PENALTY=0.095629
PRIMER_RIGHT_37_PENALTY=2.562082
PRIMER_LEFT_37_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_37_SEQUENCE=GAGCCAATGAAAGAATCCTGAA
PRIMER_LEFT_37=636,20
PRIMER_RIGHT_37=1486,22
PRIMER_LEFT_37_TM=60.096
PRIMER_RIGHT_37_TM=60.562
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.2000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=851
PRIMER_PAIR_38_PENALTY=2.661557
PRIMER_LEFT_38_PENALTY=1.066570
PRIMER_RIGHT_38_PENALTY=1.594988
PRIMER_LEFT_38_SEQUENCE=TCCAACACCATCCGCTACTC
PRIMER_RIGHT_38_SEQUENCE=TGTCTCCACGTGACAAAACCT
PRIMER_LEFT_38=447,20
PRIMER_RIGHT_38=1438,21
PRIMER_LEFT_38_TM=61.067
PRIMER_RIGHT_38_TM=60.595
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.4000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=992
PRIMER_PAIR_39_PENALTY=2.661557
PRIMER_LEFT_39_PENALTY=1.066570
PRIMER_RIGHT_39_PENALTY=1.594988
PRIMER_LEFT_39_SEQUENCE=TCCAACACCATCCGCTACTC
PRIMER_RIGHT_39_SEQUENCE=CTGTCTCCACGTGACAAAACC
PRIMER_LEFT_39=447,20
PRIMER_RIGHT_39=1439,21
PRIMER_LEFT_39_TM=61.067
PRIMER_RIGHT_39_TM=60.595
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=5.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=993
PRIMER_PAIR_40_PENALTY=2.669071
PRIMER_LEFT_40_PENALTY=2.240205
PRIMER_RIGHT_40_PENALTY=0.428866
PRIMER_LEFT_40_SEQUENCE=CCGGATTTGTATTCGTCGCT
PRIMER_RIGHT_40_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_40=635,20
PRIMER_RIGHT_40=1438,20
PRIMER_LEFT_40_TM=62.240
PRIMER_RIGHT_40_TM=59.571
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=804
PRIMER_PAIR_41_PENALTY=2.680218
PRIMER_LEFT_41_PENALTY=0.668065
PRIMER_RIGHT_41_PENALTY=2.012153
PRIMER_LEFT_41_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_41_SEQUENCE=CCTTTCTCACCAAACCAAAGAC
PRIMER_LEFT_41=528,20
PRIMER_RIGHT_41=1410,22
PRIMER_LEFT_41_TM=60.668
PRIMER_RIGHT_41_TM=60.012
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=883
PRIMER_PAIR_42_PENALTY=2.680218
PRIMER_LEFT_42_PENALTY=0.668065
PRIMER_RIGHT_42_PENALTY=2.012153
PRIMER_LEFT_42_SEQUENCE=CGCTCCTCCAACTTCTCTCA
PRIMER_RIGHT_42_SEQUENCE=ACCTTTCTCACCAAACCAAAGA
PRIMER_LEFT_42=528,20
PRIMER_RIGHT_42=1411,22
PRIMER_LEFT_42_TM=60.668
PRIMER_RIGHT_42_TM=60.012
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=884
PRIMER_PAIR_43_PENALTY=2.698268
PRIMER_LEFT_43_PENALTY=0.003322
PRIMER_RIGHT_43_PENALTY=2.694946
PRIMER_LEFT_43_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_43_SEQUENCE=CCTTTCTCACCAAACCAAAG
PRIMER_LEFT_43=445,20
PRIMER_RIGHT_43=1410,20
PRIMER_LEFT_43_TM=59.997
PRIMER_RIGHT_43_TM=57.305
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=7.3000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=2.725077
PRIMER_LEFT_44_PENALTY=2.296211
PRIMER_RIGHT_44_PENALTY=0.428866
PRIMER_LEFT_44_SEQUENCE=ACACCATCCGCTACTCCCAC
PRIMER_RIGHT_44_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_44=451,20
PRIMER_RIGHT_44=1438,20
PRIMER_LEFT_44_TM=62.296
PRIMER_RIGHT_44_TM=59.571
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=988
PRIMER_PAIR_45_PENALTY=2.726068
PRIMER_LEFT_45_PENALTY=0.031122
PRIMER_RIGHT_45_PENALTY=2.694946
PRIMER_LEFT_45_SEQUENCE=TGGATCTTTAGCCGGATTTG
PRIMER_RIGHT_45_SEQUENCE=CCTTTCTCACCAAACCAAAG
PRIMER_LEFT_45=624,20
PRIMER_RIGHT_45=1410,20
PRIMER_LEFT_45_TM=60.031
PRIMER_RIGHT_45_TM=57.305
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.2000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=787
PRIMER_PAIR_46_PENALTY=2.738136
PRIMER_LEFT_46_PENALTY=2.309270
PRIMER_RIGHT_46_PENALTY=0.428866
PRIMER_LEFT_46_SEQUENCE=AACTCCAACACCATCCGCTAC
PRIMER_RIGHT_46_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_46=444,21
PRIMER_RIGHT_46=1438,20
PRIMER_LEFT_46_TM=61.309
PRIMER_RIGHT_46_TM=59.571
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=995
PRIMER_PAIR_47_PENALTY=2.789804
PRIMER_LEFT_47_PENALTY=0.003322
PRIMER_RIGHT_47_PENALTY=2.786482
PRIMER_LEFT_47_SEQUENCE=ACTCCAACACCATCCGCTAC
PRIMER_RIGHT_47_SEQUENCE=ACCTTTCTCACCAAACCAAAG
PRIMER_LEFT_47=445,20
PRIMER_RIGHT_47=1411,21
PRIMER_LEFT_47_TM=59.997
PRIMER_RIGHT_47_TM=58.214
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=2.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=967
PRIMER_PAIR_48_PENALTY=2.790575
PRIMER_LEFT_48_PENALTY=0.095629
PRIMER_RIGHT_48_PENALTY=2.694946
PRIMER_LEFT_48_SEQUENCE=CGGATTTGTATTCGTCGCTT
PRIMER_RIGHT_48_SEQUENCE=CCTTTCTCACCAAACCAAAG
PRIMER_LEFT_48=636,20
PRIMER_RIGHT_48=1410,20
PRIMER_LEFT_48_TM=60.096
PRIMER_RIGHT_48_TM=57.305
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=10.2000
PRIMER_RIGHT_48_END_STABILITY=7.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=775
PRIMER_PAIR_49_PENALTY=2.793692
PRIMER_LEFT_49_PENALTY=2.364827
PRIMER_RIGHT_49_PENALTY=0.428866
PRIMER_LEFT_49_SEQUENCE=GGATTTGTATTCGTCGCTTCTT
PRIMER_RIGHT_49_SEQUENCE=TGTCTCCACGTGACAAAACC
PRIMER_LEFT_49=637,22
PRIMER_RIGHT_49=1438,20
PRIMER_LEFT_49_TM=59.635
PRIMER_RIGHT_49_TM=59.571
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=802
=
SEQUENCE_ID=MAGIC.105_4_6580387_6580416_po-0_bur-0_159884_25551_TRUE
SEQUENCE_TARGET=950,129
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAAACNCTATNTAATATTAACATTAATTCTCAAAATCCTAATATTTTTNTCTATCAAAATCATATAGTCAACTCATAAGAATGANAAAATACTAACTAAGTATTGTAATAATAATATTAATCCGAAAGTTTTAAGTGATTCNAAACATATTTTTTNGTTGTAATTTTGTCATATTACNNNNNNNTGGTTTTTTATGAANNAAATGTATGAAGTAANAAACATTTAGCTTAACAATTTATTTANTTTGAATAATTGNGTAANCTTTTATATATGGTTTTTATTATAAATTATATTATATTATNTTATATTTGATCATATAATATAATNTATAANAAAAACNNNNNATATATATATATTAAAAATTACAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACTGGNTCACCGCNTGNATAACGCNNTGCTCTTTNTTNCTNNAACATTGNCTATAACTANAAGATTTACATTGTGTNGTTGTTCAACANAAATCNNTTNNTCCTATGANNTTTTTATACCTTTTATCTAACTATGAATCTCACACTATGAAACATGGAACTGGACATAGTANAGAAGGAGAAGGANAAATTCATNAGNGACTTCTTNAACGAAAATTAGCAAAAGTAAAAGTCAAAGCTACTTAGGAGCTCAAGTGGTGAGAANAAGTGGGGTTTGTTCATCGCCGANAANCTGTTATGGCATTTTTATAATGGAACCATTTGAAAATAAATTAGGGTANNNATATNTATATNATTAANTGTGACATTTGNAACCNGATTNATCGAGTTTGACANAAAACAGCNNNAACTTTTCATTNTTGAACANANAAANAGTGANCCAAACTGATTGTGGTAATCCANTTGGCCATAGCGGAGACTTGTCTTGTTTTTGACCTAAATACTATCTCCTGNTTTTNNNAAANAGGAAATTANAAAAGCAACAAAGAAGTGANTGTGGAATCATTTAAACTTAGAGAGAGTTGGNTTTCGAGAAGGANAAGACCTNGATTTGAAGTTTGAGGNTATCATTGACAAGGAAACCTTNGAAACCCCANCCANNNTTTGTTNCATTNGGCCATNCCCGCNCTNTAAGAACAAAACAAAAGTTAAGTTTAACAANTTNTATGTTTNAAATAANCCTAAATANTTTAGTANTAGACGATGAAAAGGAGATATCAAACCAAGACACCAACCTTGTTTCTCCACAANGGTAGATCGGCTCTNGTCAATCACTCGTAACTTGGCTTTCACGTAACCCTTTTCATTTGACTCACTCAACAAAAGAGTGACAACGAATNACCGATTCCNGATCTAACTCCATTTGGATACACTTTCGANACCCTACACAAGTAGTACANACNCTTCACATTAAACCCAATGTGAGCCCTCATGGTGAAGTAGAGGGTCTAACATTAAACCNATTTTTTNAAAACTCNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.812366
PRIMER_LEFT_0_PENALTY=2.421595
PRIMER_RIGHT_0_PENALTY=0.390771
PRIMER_LEFT_0_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_0_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_0=868,20
PRIMER_RIGHT_0=1484,20
PRIMER_LEFT_0_TM=57.578
PRIMER_RIGHT_0_TM=59.609
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=617
PRIMER_PAIR_1_PENALTY=3.183235
PRIMER_LEFT_1_PENALTY=2.421595
PRIMER_RIGHT_1_PENALTY=0.761640
PRIMER_LEFT_1_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_1_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_1=868,20
PRIMER_RIGHT_1=1483,20
PRIMER_LEFT_1_TM=57.578
PRIMER_RIGHT_1_TM=59.238
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=616
PRIMER_PAIR_2_PENALTY=3.269197
PRIMER_LEFT_2_PENALTY=2.878426
PRIMER_RIGHT_2_PENALTY=0.390771
PRIMER_LEFT_2_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_2_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_2=866,22
PRIMER_RIGHT_2=1484,20
PRIMER_LEFT_2_TM=59.122
PRIMER_RIGHT_2_TM=59.609
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=619
PRIMER_PAIR_3_PENALTY=3.320657
PRIMER_LEFT_3_PENALTY=2.421595
PRIMER_RIGHT_3_PENALTY=0.899062
PRIMER_LEFT_3_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_3_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_3=868,20
PRIMER_RIGHT_3=1482,20
PRIMER_LEFT_3_TM=57.578
PRIMER_RIGHT_3_TM=59.101
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=615
PRIMER_PAIR_4_PENALTY=3.451799
PRIMER_LEFT_4_PENALTY=3.061028
PRIMER_RIGHT_4_PENALTY=0.390771
PRIMER_LEFT_4_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_4_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_4=865,23
PRIMER_RIGHT_4=1484,20
PRIMER_LEFT_4_TM=60.061
PRIMER_RIGHT_4_TM=59.609
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=620
PRIMER_PAIR_5_PENALTY=3.468567
PRIMER_LEFT_5_PENALTY=2.421595
PRIMER_RIGHT_5_PENALTY=1.046971
PRIMER_LEFT_5_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_5_SEQUENCE=AGGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_5=868,20
PRIMER_RIGHT_5=1483,21
PRIMER_LEFT_5_TM=57.578
PRIMER_RIGHT_5_TM=60.047
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=616
PRIMER_PAIR_6_PENALTY=3.640066
PRIMER_LEFT_6_PENALTY=2.878426
PRIMER_RIGHT_6_PENALTY=0.761640
PRIMER_LEFT_6_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_6_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_6=866,22
PRIMER_RIGHT_6=1483,20
PRIMER_LEFT_6_TM=59.122
PRIMER_RIGHT_6_TM=59.238
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=618
PRIMER_PAIR_7_PENALTY=3.646840
PRIMER_LEFT_7_PENALTY=3.256069
PRIMER_RIGHT_7_PENALTY=0.390771
PRIMER_LEFT_7_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_7_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_7=865,22
PRIMER_RIGHT_7=1484,20
PRIMER_LEFT_7_TM=58.744
PRIMER_RIGHT_7_TM=59.609
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=10.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=620
PRIMER_PAIR_8_PENALTY=3.704345
PRIMER_LEFT_8_PENALTY=3.313575
PRIMER_RIGHT_8_PENALTY=0.390771
PRIMER_LEFT_8_SEQUENCE=TAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_8_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_8=867,21
PRIMER_RIGHT_8=1484,20
PRIMER_LEFT_8_TM=57.686
PRIMER_RIGHT_8_TM=59.609
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=618
PRIMER_PAIR_9_PENALTY=3.704345
PRIMER_LEFT_9_PENALTY=3.313575
PRIMER_RIGHT_9_PENALTY=0.390771
PRIMER_LEFT_9_SEQUENCE=TTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_9_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_9=866,21
PRIMER_RIGHT_9=1484,20
PRIMER_LEFT_9_TM=57.686
PRIMER_RIGHT_9_TM=59.609
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=10.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=619
PRIMER_PAIR_10_PENALTY=3.777488
PRIMER_LEFT_10_PENALTY=2.878426
PRIMER_RIGHT_10_PENALTY=0.899062
PRIMER_LEFT_10_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_10_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_10=866,22
PRIMER_RIGHT_10=1482,20
PRIMER_LEFT_10_TM=59.122
PRIMER_RIGHT_10_TM=59.101
PRIMER_LEFT_10_GC_PERCENT=45.455
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=617
PRIMER_PAIR_11_PENALTY=3.812842
PRIMER_LEFT_11_PENALTY=3.422071
PRIMER_RIGHT_11_PENALTY=0.390771
PRIMER_LEFT_11_SEQUENCE=ACTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_11_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_11=864,23
PRIMER_RIGHT_11=1484,20
PRIMER_LEFT_11_TM=59.578
PRIMER_RIGHT_11_TM=59.609
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=10.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=621
PRIMER_PAIR_12_PENALTY=3.822668
PRIMER_LEFT_12_PENALTY=3.061028
PRIMER_RIGHT_12_PENALTY=0.761640
PRIMER_LEFT_12_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_12_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_12=865,23
PRIMER_RIGHT_12=1483,20
PRIMER_LEFT_12_TM=60.061
PRIMER_RIGHT_12_TM=59.238
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=619
PRIMER_PAIR_13_PENALTY=3.843020
PRIMER_LEFT_13_PENALTY=2.421595
PRIMER_RIGHT_13_PENALTY=1.421425
PRIMER_LEFT_13_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_13_SEQUENCE=CGTGAAAGCCAAGTTACGAG
PRIMER_LEFT_13=868,20
PRIMER_RIGHT_13=1476,20
PRIMER_LEFT_13_TM=57.578
PRIMER_RIGHT_13_TM=58.579
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=609
PRIMER_PAIR_14_PENALTY=3.850662
PRIMER_LEFT_14_PENALTY=2.421595
PRIMER_RIGHT_14_PENALTY=1.429067
PRIMER_LEFT_14_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_14_SEQUENCE=CAAGGTTGGTGTCTTGGTTTG
PRIMER_LEFT_14=868,20
PRIMER_RIGHT_14=1421,21
PRIMER_LEFT_14_TM=57.578
PRIMER_RIGHT_14_TM=60.429
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=554
PRIMER_PAIR_15_PENALTY=3.925397
PRIMER_LEFT_15_PENALTY=2.878426
PRIMER_RIGHT_15_PENALTY=1.046971
PRIMER_LEFT_15_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_15_SEQUENCE=AGGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_15=866,22
PRIMER_RIGHT_15=1483,21
PRIMER_LEFT_15_TM=59.122
PRIMER_RIGHT_15_TM=60.047
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=618
PRIMER_PAIR_16_PENALTY=3.939674
PRIMER_LEFT_16_PENALTY=2.421595
PRIMER_RIGHT_16_PENALTY=1.518079
PRIMER_LEFT_16_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_16_SEQUENCE=AAGGTTGGTGTCTTGGTTTGA
PRIMER_LEFT_16=868,20
PRIMER_RIGHT_16=1420,21
PRIMER_LEFT_16_TM=57.578
PRIMER_RIGHT_16_TM=59.482
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=553
PRIMER_PAIR_17_PENALTY=3.939674
PRIMER_LEFT_17_PENALTY=2.421595
PRIMER_RIGHT_17_PENALTY=1.518079
PRIMER_LEFT_17_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_17_SEQUENCE=GAAACAAGGTTGGTGTCTTGG
PRIMER_LEFT_17=868,20
PRIMER_RIGHT_17=1425,21
PRIMER_LEFT_17_TM=57.578
PRIMER_RIGHT_17_TM=59.482
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=558
PRIMER_PAIR_18_PENALTY=3.941011
PRIMER_LEFT_18_PENALTY=2.421595
PRIMER_RIGHT_18_PENALTY=1.519416
PRIMER_LEFT_18_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_18_SEQUENCE=AAGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_18=868,20
PRIMER_RIGHT_18=1484,21
PRIMER_LEFT_18_TM=57.578
PRIMER_RIGHT_18_TM=60.519
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=617
PRIMER_PAIR_19_PENALTY=3.960090
PRIMER_LEFT_19_PENALTY=3.061028
PRIMER_RIGHT_19_PENALTY=0.899062
PRIMER_LEFT_19_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_19_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_19=865,23
PRIMER_RIGHT_19=1482,20
PRIMER_LEFT_19_TM=60.061
PRIMER_RIGHT_19_TM=59.101
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=618
PRIMER_PAIR_20_PENALTY=3.980171
PRIMER_LEFT_20_PENALTY=3.589400
PRIMER_RIGHT_20_PENALTY=0.390771
PRIMER_LEFT_20_SEQUENCE=CACACTATGAAACATGGAACTGG
PRIMER_RIGHT_20_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_20=765,23
PRIMER_RIGHT_20=1484,20
PRIMER_LEFT_20_TM=59.411
PRIMER_RIGHT_20_TM=59.609
PRIMER_LEFT_20_GC_PERCENT=43.478
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=10.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=720
PRIMER_PAIR_21_PENALTY=3.987986
PRIMER_LEFT_21_PENALTY=2.421595
PRIMER_RIGHT_21_PENALTY=1.566391
PRIMER_LEFT_21_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_21_SEQUENCE=ACGTGAAAGCCAAGTTACGAG
PRIMER_LEFT_21=868,20
PRIMER_RIGHT_21=1477,21
PRIMER_LEFT_21_TM=57.578
PRIMER_RIGHT_21_TM=59.434
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=610
PRIMER_PAIR_22_PENALTY=3.989566
PRIMER_LEFT_22_PENALTY=2.421595
PRIMER_RIGHT_22_PENALTY=1.567971
PRIMER_LEFT_22_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_22_SEQUENCE=ACGTGAAAGCCAAGTTACGA
PRIMER_LEFT_22=868,20
PRIMER_RIGHT_22=1477,20
PRIMER_LEFT_22_TM=57.578
PRIMER_RIGHT_22_TM=58.432
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=610
PRIMER_PAIR_23_PENALTY=4.017709
PRIMER_LEFT_23_PENALTY=3.256069
PRIMER_RIGHT_23_PENALTY=0.761640
PRIMER_LEFT_23_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_23_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_23=865,22
PRIMER_RIGHT_23=1483,20
PRIMER_LEFT_23_TM=58.744
PRIMER_RIGHT_23_TM=59.238
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=619
PRIMER_PAIR_24_PENALTY=4.075214
PRIMER_LEFT_24_PENALTY=3.313575
PRIMER_RIGHT_24_PENALTY=0.761640
PRIMER_LEFT_24_SEQUENCE=TAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_24_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_24=867,21
PRIMER_RIGHT_24=1483,20
PRIMER_LEFT_24_TM=57.686
PRIMER_RIGHT_24_TM=59.238
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=617
PRIMER_PAIR_25_PENALTY=4.075214
PRIMER_LEFT_25_PENALTY=3.313575
PRIMER_RIGHT_25_PENALTY=0.761640
PRIMER_LEFT_25_SEQUENCE=TTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_25_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_25=866,21
PRIMER_RIGHT_25=1483,20
PRIMER_LEFT_25_TM=57.686
PRIMER_RIGHT_25_TM=59.238
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=618
PRIMER_PAIR_26_PENALTY=4.105399
PRIMER_LEFT_26_PENALTY=2.421595
PRIMER_RIGHT_26_PENALTY=1.683804
PRIMER_LEFT_26_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_26_SEQUENCE=ATGAGGGCTCACATTGGGTT
PRIMER_LEFT_26=868,20
PRIMER_RIGHT_26=1615,20
PRIMER_LEFT_26_TM=57.578
PRIMER_RIGHT_26_TM=61.684
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=9.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=748
PRIMER_PAIR_27_PENALTY=4.108000
PRIMER_LEFT_27_PENALTY=3.061028
PRIMER_RIGHT_27_PENALTY=1.046971
PRIMER_LEFT_27_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_27_SEQUENCE=AGGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_27=865,23
PRIMER_RIGHT_27=1483,21
PRIMER_LEFT_27_TM=60.061
PRIMER_RIGHT_27_TM=60.047
PRIMER_LEFT_27_GC_PERCENT=47.826
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=619
PRIMER_PAIR_28_PENALTY=4.155131
PRIMER_LEFT_28_PENALTY=3.256069
PRIMER_RIGHT_28_PENALTY=0.899062
PRIMER_LEFT_28_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_28_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_28=865,22
PRIMER_RIGHT_28=1482,20
PRIMER_LEFT_28_TM=58.744
PRIMER_RIGHT_28_TM=59.101
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=618
PRIMER_PAIR_29_PENALTY=4.183711
PRIMER_LEFT_29_PENALTY=3.422071
PRIMER_RIGHT_29_PENALTY=0.761640
PRIMER_LEFT_29_SEQUENCE=ACTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_29_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_29=864,23
PRIMER_RIGHT_29=1483,20
PRIMER_LEFT_29_TM=59.578
PRIMER_RIGHT_29_TM=59.238
PRIMER_LEFT_29_GC_PERCENT=47.826
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=620
PRIMER_PAIR_30_PENALTY=4.212636
PRIMER_LEFT_30_PENALTY=3.313575
PRIMER_RIGHT_30_PENALTY=0.899062
PRIMER_LEFT_30_SEQUENCE=TAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_30_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_30=867,21
PRIMER_RIGHT_30=1482,20
PRIMER_LEFT_30_TM=57.686
PRIMER_RIGHT_30_TM=59.101
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=616
PRIMER_PAIR_31_PENALTY=4.212636
PRIMER_LEFT_31_PENALTY=3.313575
PRIMER_RIGHT_31_PENALTY=0.899062
PRIMER_LEFT_31_SEQUENCE=TTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_31_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_31=866,21
PRIMER_RIGHT_31=1482,20
PRIMER_LEFT_31_TM=57.686
PRIMER_RIGHT_31_TM=59.101
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=617
PRIMER_PAIR_32_PENALTY=4.284509
PRIMER_LEFT_32_PENALTY=2.421595
PRIMER_RIGHT_32_PENALTY=1.862914
PRIMER_LEFT_32_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_32_SEQUENCE=AAAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_32=868,20
PRIMER_RIGHT_32=1485,21
PRIMER_LEFT_32_TM=57.578
PRIMER_RIGHT_32_TM=60.863
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=10.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=618
PRIMER_PAIR_33_PENALTY=4.299851
PRIMER_LEFT_33_PENALTY=2.878426
PRIMER_RIGHT_33_PENALTY=1.421425
PRIMER_LEFT_33_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_33_SEQUENCE=CGTGAAAGCCAAGTTACGAG
PRIMER_LEFT_33=866,22
PRIMER_RIGHT_33=1476,20
PRIMER_LEFT_33_TM=59.122
PRIMER_RIGHT_33_TM=58.579
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=8.1000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=611
PRIMER_PAIR_34_PENALTY=4.303041
PRIMER_LEFT_34_PENALTY=3.256069
PRIMER_RIGHT_34_PENALTY=1.046971
PRIMER_LEFT_34_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_34_SEQUENCE=AGGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_34=865,22
PRIMER_RIGHT_34=1483,21
PRIMER_LEFT_34_TM=58.744
PRIMER_RIGHT_34_TM=60.047
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=619
PRIMER_PAIR_35_PENALTY=4.307493
PRIMER_LEFT_35_PENALTY=2.878426
PRIMER_RIGHT_35_PENALTY=1.429067
PRIMER_LEFT_35_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_35_SEQUENCE=CAAGGTTGGTGTCTTGGTTTG
PRIMER_LEFT_35=866,22
PRIMER_RIGHT_35=1421,21
PRIMER_LEFT_35_TM=59.122
PRIMER_RIGHT_35_TM=60.429
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=556
PRIMER_PAIR_36_PENALTY=4.312118
PRIMER_LEFT_36_PENALTY=3.921347
PRIMER_RIGHT_36_PENALTY=0.390771
PRIMER_LEFT_36_SEQUENCE=TAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_36_SEQUENCE=AAGGGTTACGTGAAAGCCAA
PRIMER_LEFT_36=867,20
PRIMER_RIGHT_36=1484,20
PRIMER_LEFT_36_TM=56.079
PRIMER_RIGHT_36_TM=59.609
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=10.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=618
PRIMER_PAIR_37_PENALTY=4.321133
PRIMER_LEFT_37_PENALTY=3.422071
PRIMER_RIGHT_37_PENALTY=0.899062
PRIMER_LEFT_37_SEQUENCE=ACTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_37_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_37=864,23
PRIMER_RIGHT_37=1482,20
PRIMER_LEFT_37_TM=59.578
PRIMER_RIGHT_37_TM=59.101
PRIMER_LEFT_37_GC_PERCENT=47.826
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=619
PRIMER_PAIR_38_PENALTY=4.351040
PRIMER_LEFT_38_PENALTY=3.589400
PRIMER_RIGHT_38_PENALTY=0.761640
PRIMER_LEFT_38_SEQUENCE=CACACTATGAAACATGGAACTGG
PRIMER_RIGHT_38_SEQUENCE=AGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_38=765,23
PRIMER_RIGHT_38=1483,20
PRIMER_LEFT_38_TM=59.411
PRIMER_RIGHT_38_TM=59.238
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=719
PRIMER_PAIR_39_PENALTY=4.360546
PRIMER_LEFT_39_PENALTY=3.313575
PRIMER_RIGHT_39_PENALTY=1.046971
PRIMER_LEFT_39_SEQUENCE=TAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_39_SEQUENCE=AGGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_39=867,21
PRIMER_RIGHT_39=1483,21
PRIMER_LEFT_39_TM=57.686
PRIMER_RIGHT_39_TM=60.047
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=617
PRIMER_PAIR_40_PENALTY=4.360546
PRIMER_LEFT_40_PENALTY=3.313575
PRIMER_RIGHT_40_PENALTY=1.046971
PRIMER_LEFT_40_SEQUENCE=TTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_40_SEQUENCE=AGGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_40=866,21
PRIMER_RIGHT_40=1483,21
PRIMER_LEFT_40_TM=57.686
PRIMER_RIGHT_40_TM=60.047
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=618
PRIMER_PAIR_41_PENALTY=4.391793
PRIMER_LEFT_41_PENALTY=2.421595
PRIMER_RIGHT_41_PENALTY=1.970198
PRIMER_LEFT_41_SEQUENCE=AGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_41_SEQUENCE=AGGTTGGTGTCTTGGTTTGA
PRIMER_LEFT_41=868,20
PRIMER_RIGHT_41=1419,20
PRIMER_LEFT_41_TM=57.578
PRIMER_RIGHT_41_TM=58.030
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.3000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=552
PRIMER_PAIR_42_PENALTY=4.396504
PRIMER_LEFT_42_PENALTY=2.878426
PRIMER_RIGHT_42_PENALTY=1.518079
PRIMER_LEFT_42_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_42_SEQUENCE=AAGGTTGGTGTCTTGGTTTGA
PRIMER_LEFT_42=866,22
PRIMER_RIGHT_42=1420,21
PRIMER_LEFT_42_TM=59.122
PRIMER_RIGHT_42_TM=59.482
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=555
PRIMER_PAIR_43_PENALTY=4.396504
PRIMER_LEFT_43_PENALTY=2.878426
PRIMER_RIGHT_43_PENALTY=1.518079
PRIMER_LEFT_43_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_43_SEQUENCE=GAAACAAGGTTGGTGTCTTGG
PRIMER_LEFT_43=866,22
PRIMER_RIGHT_43=1425,21
PRIMER_LEFT_43_TM=59.122
PRIMER_RIGHT_43_TM=59.482
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=560
PRIMER_PAIR_44_PENALTY=4.397842
PRIMER_LEFT_44_PENALTY=2.878426
PRIMER_RIGHT_44_PENALTY=1.519416
PRIMER_LEFT_44_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_44_SEQUENCE=AAGGGTTACGTGAAAGCCAAG
PRIMER_LEFT_44=866,22
PRIMER_RIGHT_44=1484,21
PRIMER_LEFT_44_TM=59.122
PRIMER_RIGHT_44_TM=60.519
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=619
PRIMER_PAIR_45_PENALTY=4.444817
PRIMER_LEFT_45_PENALTY=2.878426
PRIMER_RIGHT_45_PENALTY=1.566391
PRIMER_LEFT_45_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_45_SEQUENCE=ACGTGAAAGCCAAGTTACGAG
PRIMER_LEFT_45=866,22
PRIMER_RIGHT_45=1477,21
PRIMER_LEFT_45_TM=59.122
PRIMER_RIGHT_45_TM=59.434
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.1000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=612
PRIMER_PAIR_46_PENALTY=4.446396
PRIMER_LEFT_46_PENALTY=2.878426
PRIMER_RIGHT_46_PENALTY=1.567971
PRIMER_LEFT_46_SEQUENCE=TTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_46_SEQUENCE=ACGTGAAAGCCAAGTTACGA
PRIMER_LEFT_46=866,22
PRIMER_RIGHT_46=1477,20
PRIMER_LEFT_46_TM=59.122
PRIMER_RIGHT_46_TM=58.432
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=612
PRIMER_PAIR_47_PENALTY=4.469043
PRIMER_LEFT_47_PENALTY=3.422071
PRIMER_RIGHT_47_PENALTY=1.046971
PRIMER_LEFT_47_SEQUENCE=ACTTAGGAGCTCAAGTGGTGAGA
PRIMER_RIGHT_47_SEQUENCE=AGGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_47=864,23
PRIMER_RIGHT_47=1483,21
PRIMER_LEFT_47_TM=59.578
PRIMER_RIGHT_47_TM=60.047
PRIMER_LEFT_47_GC_PERCENT=47.826
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=620
PRIMER_PAIR_48_PENALTY=4.482453
PRIMER_LEFT_48_PENALTY=3.061028
PRIMER_RIGHT_48_PENALTY=1.421425
PRIMER_LEFT_48_SEQUENCE=CTTAGGAGCTCAAGTGGTGAGAA
PRIMER_RIGHT_48_SEQUENCE=CGTGAAAGCCAAGTTACGAG
PRIMER_LEFT_48=865,23
PRIMER_RIGHT_48=1476,20
PRIMER_LEFT_48_TM=60.061
PRIMER_RIGHT_48_TM=58.579
PRIMER_LEFT_48_GC_PERCENT=47.826
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=612
PRIMER_PAIR_49_PENALTY=4.488462
PRIMER_LEFT_49_PENALTY=3.589400
PRIMER_RIGHT_49_PENALTY=0.899062
PRIMER_LEFT_49_SEQUENCE=CACACTATGAAACATGGAACTGG
PRIMER_RIGHT_49_SEQUENCE=GGGTTACGTGAAAGCCAAGT
PRIMER_LEFT_49=765,23
PRIMER_RIGHT_49=1482,20
PRIMER_LEFT_49_TM=59.411
PRIMER_RIGHT_49_TM=59.101
PRIMER_LEFT_49_GC_PERCENT=43.478
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=718
=
SEQUENCE_ID=MAGIC.426_1_24177167_24177236_mt-0_rsch-4_1475_847402_TRUE
SEQUENCE_TARGET=950,169
SEQUENCE_TEMPLATE=NNANNNNNNNNNGGNNNNNNGTGNNNANTTGNGNTGAATNNGNNTNNNNATNNNTTNNNNNNNNNNANNAGTNTTNANNNCTGTGGNNAANGANNNNTCNANNNTNCNNTTNNNNTNTGNNNNNNNTCNGNNNNTNNNNAGTTNNNNNNNNTTNNNTNNGGANNNNNNNNTTGNNCNNNTTAANNNANNTTGTTCNNGNTTNAANNNNTNNNNNANTNNNNTTCGNTNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNTNNNNNNNNNTNNNNNANNNNNNNNNNNNNTNNNNANCNANNNNNNNNNNNTNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNTNNNNAGNNNNNNNNNNNNNNTNNNNNTNNNNNTNTNNGNNNNANNNNNNNGNNNNNNNCNNNNNTNNNNGNNNNNNNNNNNTNNNNNCNCNNCNNCNCCNNTNNTCNNCNNTNNNCTNCTCNCNNNNNCNCCCNCNNCNTNNNNNNCTCNNNNTNNNNCTCNNNTNCNCNNNCNCNCNNNTNNCCNNNNNNNNCNNNNTNTCCNNNNNNNNNCTNTCNTCNNNNCNGNNNNNNCNNTTNNNNNNCNGANNTNNNNNNNNNNTNNCNNNNNNTCNTNNNNNNNNATNNNNCNNCNTNNNNTNNTNTNGNNGNNNTNNATNNNNTNNNNNGNNTNNNNNNNTNNNNNNNNNNNNNNTNNNNNANNNNTNNTGNNNNTNNTANNNNNGNTNTNNNNNTNNNNTTNNNNCNTNNACTNNNNNNNNNNNTNNANNNATTNNAGNNNNNTNCNNNNNTGNTANNNNANTNNNNNNNTNNNNTNNCTANNNNNTNNNTNNTTNNTNTNNNTNGNNNNNNNNNNNNTNANANNNNNNTNNTNNNNNTNNTNNNNNGTNNNNNTNTNNNNATGNNNNNCNNNNNNNNNNNNNNNNTNNNNNNNNNNNNTTGNNNNNNANNNNNNTNNNNNNGTNNNNNCNATNNNANTNGTTCTNNATNTTCNTNNANNGTGNNNATNANGTTNAAANCNANTNNNANTNTTCTNCNTTTCNTANANAGNGNCNANGCNNNTNNNNGNNNCTCCANNNTTNNNNGGNTCNNNTNNCCANNNTTNTNNNTNNNNNNAAGANANNNNNNCNATTGNGTGCNNNNNNNTTNTGTNNTTNTTNCNANNNTTNTNNNTCCNGGNNAANANCNTCNTNNNTNGANNNNTTNNTNNNTCTGGTNTGTNTNNNANNTTNTNTNAAANTCTNNNCANGNNNNNTNNTNCTTNGNTTTNNNNTTANANNTGANNNNNNTNNNGNNNNNTCNATNNNNTACATNNTNNTANNTNTGNTNNNTNNNTTNNATNANANTCNTTCNNNTNACTTNANNNTTNGNTTTNNTNATNNNTGNNNNNNNCNNNTTTTCTNTGTTGATNNNNNNNANAAANANGTNANTNGNTNGNNNTCANNCTNNCTTNNNNTNTGNNNNTTGNNANCTGCNGCTNNNATNNTTNTTCTTNTTGATNGNNANNATCNGGANNANGTCANNNNNTNNTCNCNNNNNCTTTNNTTNTGNNNGTNGAGNNNGCATANNNNNNNNNTNTANNNTNTGNNCNTCTNNNTTNNNANGNGGNAANNNNNNTTNNNNNNGATNAANTNANACNNNTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNTNGNCNAGTTNNCNTNTNTNGGNATNTNANTNNGCNNGTNNCNNNNNATNANTNATANNANNNTNCNTNNNTNCNNTNTNNNTNNANNANNNTNNNNTANTNCNNCGNTNNNNNNANTNTTATATTGAAGNTAGACCTTANTNGTANANNNNNNNNNNACACCTGTATACTATGAAAACTGGTGNGCCNNGAGCATATTTGACCTGANTNTNTCCTNTCATATTTCCNTGNTGTTGCNTCTGAGACTGANCTGCNTCNTATNTTTGCAGGGTGGTGNCTTATGGTTCCAGAANTTGAGTGATCTTCCTGGNGGNTCTTTTGGACCAGTCTTNCCAATTTTAATTGCTAC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_6016402_6016904_bur-0_hi-0_90803_2273748_TRUE
SEQUENCE_TARGET=950,602
SEQUENCE_TEMPLATE=CCGACTGATTTCAAATCCAAATCTCCCCAGAACCCTAATCCCAAACCTTCCTCATGCCCATTTTGCATCGGTCGTGAGCAAGAGTGTGCCCCGGAGNTATTTCGTGTGCCAGATCATGACCCGAATTGGAAACTCCGGGTCATCGAGAATCTGTACCCGGCTCTTAGTAGGAATCTCGAGACCCAATCGACCCAANCTGAGACGGGAACAAGTCGGACAATAGTCGGATTCGGATTCCACGACGTAGTGATCGAATCCCCTGTTCATTCGATTCAGCTCTCCGATATNGATCCGGTGGGTATCGGCGATNTCTTGATCGCTTATAAGAAGAGGATCAATCAGATTGCGCAACATGATTCCATCAATTACATCCAGGTAAGCATTNTCCATAGATCCCAATGTAGTAGCTNNATTAGTGTGNATTTATACATTGTTTAGCCGTCAATACTTGTGTTATGTGTGGATATTTTGTGTTGCTTGCATAGTTACATTCATCTGCTACGTTTGTTTTCGTATGTTGAAGGTTTTCAAGAACCAAGGTGCGTCGGCTGGAGCATCAATGAGTCACTCCCACAGTCAAATGATGGCTCTACCAGTTGTTCCTCCAACGGTTTCTTCACGCCTTGATGGTACTAAAGATTATTTCGAGGAGACTGGGAAATGTTGTCTCTGTGAAGCTANATCAAAACATTTTGTGATCGATGAGTCGTCTCATTTTGTTTCTGTTGNTCCTTTTGCTGCTACGTATCCCTTTGAGATTTGGATTATTCCGAAAGATCATTCTTCCCACTTCCATCATCTCGNTGACGTTAAGGTTACCTTTTACTGATCTACTATGTTCCATTTATGGTTTGGTTAGATGATCACCTGACTCNCTTTGTTTTGATGATTATCAGGCTGTTGACCTTGGAGGACTTCTGAAACNCATGCTTCAGAAGATANCAAAGCANCTNAANGATCCNCCGTATAACTATATGATCCATACTTCTCCNCTCAAGGTNACAGAATCACAANTACCTTATACGCACTGGTTCTTACAGATTGTACCTCAGCTTTCTGGGGTAGGTGGGTTTGAGATTGGCACAGGTTGTTACATAAACCCTGTTTTCCCAGAGGATGTAGCAAAGGTTATGCGGGAAGTTAGCCTTACTTGAACCCGNTTTTTTAGTCTCAACCTAAAGTTCAGCTGGAAGTGATTAGTGTTGAGTTATTGGCCTNATNTGCAAGTCTCTANAAAACACTATTCAATTGTNTTTTTNTCCTCTGTTGAAGAGGAAGATGTTTCAGCTTGATTCAAATAACACTGATGTNAACAATGCATGTACTGTAGTTCTTTGTAAAACAAGCTCATATCAGGGNATTAAGAAGATTCTTTGATCAATCAGCTTTTAAATTCAGCATCAAACCAATTAACCNNNNNNNNNNNNNNNNNNCGAACCAAACCAGACGATGCTTGANGGCGTCGTTNAAAATGNTTNTAAAGGGNAATTNTNAGAGCNNCTNTCTCAAAAACTCNAANCAGCGAGTGAAAAAAACACAGAGAGCTTCCTCACTTGCTTATAGCTTAGGGTTTACGTTCAAGGCNTAGATCTTCGTCGGAAACAATGGGNATCTCTACGCAGTCTCATGGAATTCAATCGATGCTTAAAGNAGGTTANAGACATCTCGCTGGTCTTGATGAGGCCGTCATCAAAAACANTGAAGCTTGCAAGGAGCTTTCCACCNTTACCCGCACTTCTCTTNGCCCCAATGGTTTGCCTCTCGTCGTCGGATTCGAAATCTATCGTTTCTGTAATGTTAGAATCTGTTTATGATTATTGTTCTGATTTAGGAATGAATAAAGACTGAGCCGATGCTTATATGCAAAGTGTGGCTAACTATGGTGCTTATAAATGGCAGATTATAATGGCTAAACCAGCAGGTGGGCCGAGGAGAGATGCAGCTGCAGCAGCCGGTGCAGGCATGGACGAAGACTAAGCTGTTATAACAATCACCTNTTAGCCTAACANCTCTTCTTCTATATTCATTAGTTTCTTTTTTNATCTTTCTTAGAAAAGTNTTTCTTATAAAAGTTTAATCTTTCTTCTGCCTGTCATTGGTTCCTCTCAACATTTGACANTGAATAGTTGTAATGACAAAGTTTTAGTTGGGTTTTAGACAAAAATGATCAANATTCTAACAAAACAATATAAGAACTAGTTNNATTCTGGCTCTAGTNNTCATTCTGGTTACACAGTAAAATTAAAGATGGAGATAACCTCCCNTAAGTAAAATGTACTAGAATTTTGAGAAGTCCAAAATCTTAAACCCCNAACGGCGTCGNTTNNNAACACTAACGCCGNCGTTTAGCAACACTAACGNCGNCTTGTTCAGTTCCACAGNNNNNNNTCAAAACAGAGTACAGTCACACAGTCAACGAAGATACNNNTAAGTCAATTTTCGTTTGACCAAANTTGATTCTTCATGGCTTCCTCAGTNTCCTCTCTCGCCGGACGAGTCGCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.163344
PRIMER_LEFT_0_PENALTY=0.656393
PRIMER_RIGHT_0_PENALTY=1.506951
PRIMER_LEFT_0_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_0_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_0=891,20
PRIMER_RIGHT_0=1682,20
PRIMER_LEFT_0_TM=60.656
PRIMER_RIGHT_0_TM=61.507
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=792
PRIMER_PAIR_1_PENALTY=2.295925
PRIMER_LEFT_1_PENALTY=0.656393
PRIMER_RIGHT_1_PENALTY=1.639532
PRIMER_LEFT_1_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_1_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_1=891,20
PRIMER_RIGHT_1=1863,21
PRIMER_LEFT_1_TM=60.656
PRIMER_RIGHT_1_TM=59.360
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=2.564671
PRIMER_LEFT_2_PENALTY=1.057719
PRIMER_RIGHT_2_PENALTY=1.506951
PRIMER_LEFT_2_SEQUENCE=TGCTGCTACGTATCCCTTTG
PRIMER_RIGHT_2_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_2=735,20
PRIMER_RIGHT_2=1682,20
PRIMER_LEFT_2_TM=58.942
PRIMER_RIGHT_2_TM=61.507
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=948
PRIMER_PAIR_3_PENALTY=2.577113
PRIMER_LEFT_3_PENALTY=0.187324
PRIMER_RIGHT_3_PENALTY=2.389789
PRIMER_LEFT_3_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_3_SEQUENCE=TTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_3=643,20
PRIMER_RIGHT_3=1631,21
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=58.610
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=989
PRIMER_PAIR_4_PENALTY=2.629518
PRIMER_LEFT_4_PENALTY=0.656393
PRIMER_RIGHT_4_PENALTY=1.973125
PRIMER_LEFT_4_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_4_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_4=891,20
PRIMER_RIGHT_4=1767,20
PRIMER_LEFT_4_TM=60.656
PRIMER_RIGHT_4_TM=61.973
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=877
PRIMER_PAIR_5_PENALTY=2.712413
PRIMER_LEFT_5_PENALTY=0.656393
PRIMER_RIGHT_5_PENALTY=2.056021
PRIMER_LEFT_5_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_5_SEQUENCE=GCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_5=891,20
PRIMER_RIGHT_5=1855,21
PRIMER_LEFT_5_TM=60.656
PRIMER_RIGHT_5_TM=58.944
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=5.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=965
PRIMER_PAIR_6_PENALTY=2.751838
PRIMER_LEFT_6_PENALTY=0.656393
PRIMER_RIGHT_6_PENALTY=2.095445
PRIMER_LEFT_6_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_6_SEQUENCE=CGGCTCAGTCTTTATTCATTCC
PRIMER_LEFT_6=891,20
PRIMER_RIGHT_6=1851,22
PRIMER_LEFT_6_TM=60.656
PRIMER_RIGHT_6_TM=60.095
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=961
PRIMER_PAIR_7_PENALTY=2.772896
PRIMER_LEFT_7_PENALTY=0.656393
PRIMER_RIGHT_7_PENALTY=2.116503
PRIMER_LEFT_7_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_7_SEQUENCE=AGCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_7=891,20
PRIMER_RIGHT_7=1856,22
PRIMER_LEFT_7_TM=60.656
PRIMER_RIGHT_7_TM=59.883
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=5.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=966
PRIMER_PAIR_8_PENALTY=2.777735
PRIMER_LEFT_8_PENALTY=1.270784
PRIMER_RIGHT_8_PENALTY=1.506951
PRIMER_LEFT_8_SEQUENCE=TTGCTGCTACGTATCCCTTTG
PRIMER_RIGHT_8_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_8=734,21
PRIMER_RIGHT_8=1682,20
PRIMER_LEFT_8_TM=60.271
PRIMER_RIGHT_8_TM=61.507
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=949
PRIMER_PAIR_9_PENALTY=2.896891
PRIMER_LEFT_9_PENALTY=1.389940
PRIMER_RIGHT_9_PENALTY=1.506951
PRIMER_LEFT_9_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_9_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_9=892,20
PRIMER_RIGHT_9=1682,20
PRIMER_LEFT_9_TM=61.390
PRIMER_RIGHT_9_TM=61.507
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=791
PRIMER_PAIR_10_PENALTY=2.929675
PRIMER_LEFT_10_PENALTY=0.656393
PRIMER_RIGHT_10_PENALTY=2.273283
PRIMER_LEFT_10_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_10_SEQUENCE=TAAGCATCGGCTCAGTCTTT
PRIMER_LEFT_10=891,20
PRIMER_RIGHT_10=1858,20
PRIMER_LEFT_10_TM=60.656
PRIMER_RIGHT_10_TM=57.727
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=968
PRIMER_PAIR_11_PENALTY=2.952360
PRIMER_LEFT_11_PENALTY=1.445408
PRIMER_RIGHT_11_PENALTY=1.506951
PRIMER_LEFT_11_SEQUENCE=TCATTCTTCCCACTTCCATCA
PRIMER_RIGHT_11_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_11=777,21
PRIMER_RIGHT_11=1682,20
PRIMER_LEFT_11_TM=60.445
PRIMER_RIGHT_11_TM=61.507
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=906
PRIMER_PAIR_12_PENALTY=3.019661
PRIMER_LEFT_12_PENALTY=1.512710
PRIMER_RIGHT_12_PENALTY=1.506951
PRIMER_LEFT_12_SEQUENCE=CATTCTTCCCACTTCCATCA
PRIMER_RIGHT_12_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_12=778,20
PRIMER_RIGHT_12=1682,20
PRIMER_LEFT_12_TM=58.487
PRIMER_RIGHT_12_TM=61.507
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=905
PRIMER_PAIR_13_PENALTY=3.029472
PRIMER_LEFT_13_PENALTY=1.389940
PRIMER_RIGHT_13_PENALTY=1.639532
PRIMER_LEFT_13_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_13_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_13=892,20
PRIMER_RIGHT_13=1863,21
PRIMER_LEFT_13_TM=61.390
PRIMER_RIGHT_13_TM=59.360
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=972
PRIMER_PAIR_14_PENALTY=3.046182
PRIMER_LEFT_14_PENALTY=0.656393
PRIMER_RIGHT_14_PENALTY=2.389789
PRIMER_LEFT_14_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_14_SEQUENCE=TTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_14=891,20
PRIMER_RIGHT_14=1631,21
PRIMER_LEFT_14_TM=60.656
PRIMER_RIGHT_14_TM=58.610
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=741
PRIMER_PAIR_15_PENALTY=3.148695
PRIMER_LEFT_15_PENALTY=1.641744
PRIMER_RIGHT_15_PENALTY=1.506951
PRIMER_LEFT_15_SEQUENCE=TATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_15_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_15=890,21
PRIMER_RIGHT_15=1682,20
PRIMER_LEFT_15_TM=60.642
PRIMER_RIGHT_15_TM=61.507
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=793
PRIMER_PAIR_16_PENALTY=3.153824
PRIMER_LEFT_16_PENALTY=0.187324
PRIMER_RIGHT_16_PENALTY=2.966501
PRIMER_LEFT_16_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_16_SEQUENCE=TCCATGAGACTGCGTAGAGA
PRIMER_LEFT_16=643,20
PRIMER_RIGHT_16=1630,20
PRIMER_LEFT_16_TM=60.187
PRIMER_RIGHT_16_TM=57.033
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=988
PRIMER_PAIR_17_PENALTY=3.154971
PRIMER_LEFT_17_PENALTY=0.088296
PRIMER_RIGHT_17_PENALTY=3.066675
PRIMER_LEFT_17_SEQUENCE=TCCTCCAACGGTTTCTTCAC
PRIMER_RIGHT_17_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_17=600,20
PRIMER_RIGHT_17=1583,20
PRIMER_LEFT_17_TM=60.088
PRIMER_RIGHT_17_TM=56.933
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.5000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=984
PRIMER_PAIR_18_PENALTY=3.182199
PRIMER_LEFT_18_PENALTY=1.675248
PRIMER_RIGHT_18_PENALTY=1.506951
PRIMER_LEFT_18_SEQUENCE=GCTGTTGACCTTGGAGGACTT
PRIMER_RIGHT_18_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_18=896,21
PRIMER_RIGHT_18=1682,20
PRIMER_LEFT_18_TM=60.675
PRIMER_RIGHT_18_TM=61.507
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=787
PRIMER_PAIR_19_PENALTY=3.225990
PRIMER_LEFT_19_PENALTY=0.187324
PRIMER_RIGHT_19_PENALTY=3.038666
PRIMER_LEFT_19_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_19_SEQUENCE=ATTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_19=643,20
PRIMER_RIGHT_19=1632,22
PRIMER_LEFT_19_TM=60.187
PRIMER_RIGHT_19_TM=58.961
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=990
PRIMER_PAIR_20_PENALTY=3.236591
PRIMER_LEFT_20_PENALTY=0.656393
PRIMER_RIGHT_20_PENALTY=2.580198
PRIMER_LEFT_20_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_20_SEQUENCE=AAGCATCGGCTCAGTCTTTATT
PRIMER_LEFT_20=891,20
PRIMER_RIGHT_20=1857,22
PRIMER_LEFT_20_TM=60.656
PRIMER_RIGHT_20_TM=59.420
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=6.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=967
PRIMER_PAIR_21_PENALTY=3.253998
PRIMER_LEFT_21_PENALTY=0.187324
PRIMER_RIGHT_21_PENALTY=3.066675
PRIMER_LEFT_21_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_21_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_21=643,20
PRIMER_RIGHT_21=1583,20
PRIMER_LEFT_21_TM=60.187
PRIMER_RIGHT_21_TM=56.933
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.2000
PRIMER_RIGHT_21_END_STABILITY=7.5000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=941
PRIMER_PAIR_22_PENALTY=3.281276
PRIMER_LEFT_22_PENALTY=1.641744
PRIMER_RIGHT_22_PENALTY=1.639532
PRIMER_LEFT_22_SEQUENCE=TATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_22_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_22=890,21
PRIMER_RIGHT_22=1863,21
PRIMER_LEFT_22_TM=60.642
PRIMER_RIGHT_22_TM=59.360
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=974
PRIMER_PAIR_23_PENALTY=3.283979
PRIMER_LEFT_23_PENALTY=1.777028
PRIMER_RIGHT_23_PENALTY=1.506951
PRIMER_LEFT_23_SEQUENCE=TGCTGCTACGTATCCCTTTGA
PRIMER_RIGHT_23_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_23=735,21
PRIMER_RIGHT_23=1682,20
PRIMER_LEFT_23_TM=60.777
PRIMER_RIGHT_23_TM=61.507
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.3000
PRIMER_RIGHT_23_END_STABILITY=8.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=948
PRIMER_PAIR_24_PENALTY=3.314780
PRIMER_LEFT_24_PENALTY=1.675248
PRIMER_RIGHT_24_PENALTY=1.639532
PRIMER_LEFT_24_SEQUENCE=GCTGTTGACCTTGGAGGACTT
PRIMER_RIGHT_24_SEQUENCE=GCATATAAGCATCGGCTCAGT
PRIMER_LEFT_24=896,21
PRIMER_RIGHT_24=1863,21
PRIMER_LEFT_24_TM=60.675
PRIMER_RIGHT_24_TM=59.360
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=968
PRIMER_PAIR_25_PENALTY=3.363065
PRIMER_LEFT_25_PENALTY=1.389940
PRIMER_RIGHT_25_PENALTY=1.973125
PRIMER_LEFT_25_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_25_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_25=892,20
PRIMER_RIGHT_25=1767,20
PRIMER_LEFT_25_TM=61.390
PRIMER_RIGHT_25_TM=61.973
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=876
PRIMER_PAIR_26_PENALTY=3.418533
PRIMER_LEFT_26_PENALTY=1.445408
PRIMER_RIGHT_26_PENALTY=1.973125
PRIMER_LEFT_26_SEQUENCE=TCATTCTTCCCACTTCCATCA
PRIMER_RIGHT_26_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_26=777,21
PRIMER_RIGHT_26=1767,20
PRIMER_LEFT_26_TM=60.445
PRIMER_RIGHT_26_TM=61.973
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.9000
PRIMER_RIGHT_26_END_STABILITY=8.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=991
PRIMER_PAIR_27_PENALTY=3.445961
PRIMER_LEFT_27_PENALTY=1.389940
PRIMER_RIGHT_27_PENALTY=2.056021
PRIMER_LEFT_27_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_27_SEQUENCE=GCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_27=892,20
PRIMER_RIGHT_27=1855,21
PRIMER_LEFT_27_TM=61.390
PRIMER_RIGHT_27_TM=58.944
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=5.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=964
PRIMER_PAIR_28_PENALTY=3.463313
PRIMER_LEFT_28_PENALTY=0.187324
PRIMER_RIGHT_28_PENALTY=3.275990
PRIMER_LEFT_28_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_28_SEQUENCE=AATTCCATGAGACTGCGTAGAGA
PRIMER_LEFT_28=643,20
PRIMER_RIGHT_28=1633,23
PRIMER_LEFT_28_TM=60.187
PRIMER_RIGHT_28_TM=60.276
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=991
PRIMER_PAIR_29_PENALTY=3.463313
PRIMER_LEFT_29_PENALTY=0.187324
PRIMER_RIGHT_29_PENALTY=3.275990
PRIMER_LEFT_29_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_29_SEQUENCE=GAATTCCATGAGACTGCGTAGAG
PRIMER_LEFT_29=643,20
PRIMER_RIGHT_29=1634,23
PRIMER_LEFT_29_TM=60.187
PRIMER_RIGHT_29_TM=60.276
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.826
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.2000
PRIMER_RIGHT_29_END_STABILITY=5.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=992
PRIMER_PAIR_30_PENALTY=3.477222
PRIMER_LEFT_30_PENALTY=1.970271
PRIMER_RIGHT_30_PENALTY=1.506951
PRIMER_LEFT_30_SEQUENCE=CGTATCCCTTTGAGATTTGGA
PRIMER_RIGHT_30_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_30=743,21
PRIMER_RIGHT_30=1682,20
PRIMER_LEFT_30_TM=59.030
PRIMER_RIGHT_30_TM=61.507
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=940
PRIMER_PAIR_31_PENALTY=3.485385
PRIMER_LEFT_31_PENALTY=1.389940
PRIMER_RIGHT_31_PENALTY=2.095445
PRIMER_LEFT_31_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_31_SEQUENCE=CGGCTCAGTCTTTATTCATTCC
PRIMER_LEFT_31=892,20
PRIMER_RIGHT_31=1851,22
PRIMER_LEFT_31_TM=61.390
PRIMER_RIGHT_31_TM=60.095
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=960
PRIMER_PAIR_32_PENALTY=3.485835
PRIMER_LEFT_32_PENALTY=1.512710
PRIMER_RIGHT_32_PENALTY=1.973125
PRIMER_LEFT_32_SEQUENCE=CATTCTTCCCACTTCCATCA
PRIMER_RIGHT_32_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_32=778,20
PRIMER_RIGHT_32=1767,20
PRIMER_LEFT_32_TM=58.487
PRIMER_RIGHT_32_TM=61.973
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=990
PRIMER_PAIR_33_PENALTY=3.496831
PRIMER_LEFT_33_PENALTY=1.989880
PRIMER_RIGHT_33_PENALTY=1.506951
PRIMER_LEFT_33_SEQUENCE=GCTGCTACGTATCCCTTTGAG
PRIMER_RIGHT_33_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_33=736,21
PRIMER_RIGHT_33=1682,20
PRIMER_LEFT_33_TM=59.010
PRIMER_RIGHT_33_TM=61.507
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=947
PRIMER_PAIR_34_PENALTY=3.498161
PRIMER_LEFT_34_PENALTY=0.431486
PRIMER_RIGHT_34_PENALTY=3.066675
PRIMER_LEFT_34_SEQUENCE=TGTTCCTCCAACGGTTTCTT
PRIMER_RIGHT_34_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_34=597,20
PRIMER_RIGHT_34=1583,20
PRIMER_LEFT_34_TM=59.569
PRIMER_RIGHT_34_TM=56.933
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.5000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=987
PRIMER_PAIR_35_PENALTY=3.498161
PRIMER_LEFT_35_PENALTY=0.431486
PRIMER_RIGHT_35_PENALTY=3.066675
PRIMER_LEFT_35_SEQUENCE=TTGTTCCTCCAACGGTTTCT
PRIMER_RIGHT_35_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_35=596,20
PRIMER_RIGHT_35=1583,20
PRIMER_LEFT_35_TM=59.569
PRIMER_RIGHT_35_TM=56.933
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.5000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=988
PRIMER_PAIR_36_PENALTY=3.506443
PRIMER_LEFT_36_PENALTY=1.389940
PRIMER_RIGHT_36_PENALTY=2.116503
PRIMER_LEFT_36_SEQUENCE=TCAGGCTGTTGACCTTGGAG
PRIMER_RIGHT_36_SEQUENCE=AGCATCGGCTCAGTCTTTATTC
PRIMER_LEFT_36=892,20
PRIMER_RIGHT_36=1856,22
PRIMER_LEFT_36_TM=61.390
PRIMER_RIGHT_36_TM=59.883
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=5.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=965
PRIMER_PAIR_37_PENALTY=3.508257
PRIMER_LEFT_37_PENALTY=2.001306
PRIMER_RIGHT_37_PENALTY=1.506951
PRIMER_LEFT_37_SEQUENCE=GCTGCTACGTATCCCTTTGA
PRIMER_RIGHT_37_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_37=736,20
PRIMER_RIGHT_37=1682,20
PRIMER_LEFT_37_TM=57.999
PRIMER_RIGHT_37_TM=61.507
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.3000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=947
PRIMER_PAIR_38_PENALTY=3.545884
PRIMER_LEFT_38_PENALTY=0.656393
PRIMER_RIGHT_38_PENALTY=2.889491
PRIMER_LEFT_38_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_38_SEQUENCE=AGCATCGGCTCAGTCTTTATT
PRIMER_LEFT_38=891,20
PRIMER_RIGHT_38=1856,21
PRIMER_LEFT_38_TM=60.656
PRIMER_RIGHT_38_TM=58.111
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=6.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=966
PRIMER_PAIR_39_PENALTY=3.545884
PRIMER_LEFT_39_PENALTY=0.656393
PRIMER_RIGHT_39_PENALTY=2.889491
PRIMER_LEFT_39_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_39_SEQUENCE=ATAAGCATCGGCTCAGTCTTT
PRIMER_LEFT_39=891,20
PRIMER_RIGHT_39=1859,21
PRIMER_LEFT_39_TM=60.656
PRIMER_RIGHT_39_TM=58.111
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=969
PRIMER_PAIR_40_PENALTY=3.566826
PRIMER_LEFT_40_PENALTY=2.059875
PRIMER_RIGHT_40_PENALTY=1.506951
PRIMER_LEFT_40_SEQUENCE=AAGATCATTCTTCCCACTTCCA
PRIMER_RIGHT_40_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_40=773,22
PRIMER_RIGHT_40=1682,20
PRIMER_LEFT_40_TM=59.940
PRIMER_RIGHT_40_TM=61.507
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=910
PRIMER_PAIR_41_PENALTY=3.595427
PRIMER_LEFT_41_PENALTY=2.088476
PRIMER_RIGHT_41_PENALTY=1.506951
PRIMER_LEFT_41_SEQUENCE=TTTGCTGCTACGTATCCCTTT
PRIMER_RIGHT_41_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_41=733,21
PRIMER_RIGHT_41=1682,20
PRIMER_LEFT_41_TM=58.912
PRIMER_RIGHT_41_TM=61.507
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=950
PRIMER_PAIR_42_PENALTY=3.595427
PRIMER_LEFT_42_PENALTY=2.088476
PRIMER_RIGHT_42_PENALTY=1.506951
PRIMER_LEFT_42_SEQUENCE=TTTTGCTGCTACGTATCCCTT
PRIMER_RIGHT_42_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_42=732,21
PRIMER_RIGHT_42=1682,20
PRIMER_LEFT_42_TM=58.912
PRIMER_RIGHT_42_TM=61.507
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=951
PRIMER_PAIR_43_PENALTY=3.598696
PRIMER_LEFT_43_PENALTY=0.656393
PRIMER_RIGHT_43_PENALTY=2.942303
PRIMER_LEFT_43_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_43_SEQUENCE=GCATCGGCTCAGTCTTTATT
PRIMER_LEFT_43=891,20
PRIMER_RIGHT_43=1855,20
PRIMER_LEFT_43_TM=60.656
PRIMER_RIGHT_43_TM=57.058
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=965
PRIMER_PAIR_44_PENALTY=3.614869
PRIMER_LEFT_44_PENALTY=1.641744
PRIMER_RIGHT_44_PENALTY=1.973125
PRIMER_LEFT_44_SEQUENCE=TATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_44_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_44=890,21
PRIMER_RIGHT_44=1767,20
PRIMER_LEFT_44_TM=60.642
PRIMER_RIGHT_44_TM=61.973
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=878
PRIMER_PAIR_45_PENALTY=3.622894
PRIMER_LEFT_45_PENALTY=0.656393
PRIMER_RIGHT_45_PENALTY=2.966501
PRIMER_LEFT_45_SEQUENCE=ATCAGGCTGTTGACCTTGGA
PRIMER_RIGHT_45_SEQUENCE=TCCATGAGACTGCGTAGAGA
PRIMER_LEFT_45=891,20
PRIMER_RIGHT_45=1630,20
PRIMER_LEFT_45_TM=60.656
PRIMER_RIGHT_45_TM=57.033
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=740
PRIMER_PAIR_46_PENALTY=3.634033
PRIMER_LEFT_46_PENALTY=0.187324
PRIMER_RIGHT_46_PENALTY=3.446709
PRIMER_LEFT_46_SEQUENCE=TTCGAGGAGACTGGGAAATG
PRIMER_RIGHT_46_SEQUENCE=TTCCATGAGACTGCGTAGAG
PRIMER_LEFT_46=643,20
PRIMER_RIGHT_46=1631,20
PRIMER_LEFT_46_TM=60.187
PRIMER_RIGHT_46_TM=56.553
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.2000
PRIMER_RIGHT_46_END_STABILITY=5.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=989
PRIMER_PAIR_47_PENALTY=3.637175
PRIMER_LEFT_47_PENALTY=0.570500
PRIMER_RIGHT_47_PENALTY=3.066675
PRIMER_LEFT_47_SEQUENCE=GTTGTTCCTCCAACGGTTTC
PRIMER_RIGHT_47_SEQUENCE=GCCTTGAACGTAAACCCTAA
PRIMER_LEFT_47=595,20
PRIMER_RIGHT_47=1583,20
PRIMER_LEFT_47_TM=59.430
PRIMER_RIGHT_47_TM=56.933
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.5000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=989
PRIMER_PAIR_48_PENALTY=3.648373
PRIMER_LEFT_48_PENALTY=1.675248
PRIMER_RIGHT_48_PENALTY=1.973125
PRIMER_LEFT_48_SEQUENCE=GCTGTTGACCTTGGAGGACTT
PRIMER_RIGHT_48_SEQUENCE=ACGACGAGAGGCAAACCATT
PRIMER_LEFT_48=896,21
PRIMER_RIGHT_48=1767,20
PRIMER_LEFT_48_TM=60.675
PRIMER_RIGHT_48_TM=61.973
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=872
PRIMER_PAIR_49_PENALTY=3.651899
PRIMER_LEFT_49_PENALTY=2.144948
PRIMER_RIGHT_49_PENALTY=1.506951
PRIMER_LEFT_49_SEQUENCE=AGGCTGTTGACCTTGGAGGA
PRIMER_RIGHT_49_SEQUENCE=CCTCATCAAGACCAGCGAGA
PRIMER_LEFT_49=894,20
PRIMER_RIGHT_49=1682,20
PRIMER_LEFT_49_TM=62.145
PRIMER_RIGHT_49_TM=61.507
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=789
=
SEQUENCE_ID=MAGIC.433_4_1349635_1349848_wu-0_oy-0_474653_262712_TRUE
SEQUENCE_TARGET=950,313
SEQUENCE_TEMPLATE=CTAGGATTTTTATTCCGTGCTGAGAAAGAAATGAAGAAGGGTACTGAGATAATTAACTCCTAGTTTATCTAAATATAATTTGGAGTTGCTCAATAGTGTCCCAATTATAGAGAAACTTATCAACATTAAAACAGAGTGGTTATTAGTTTTCTTGCTTTTCAGATTTTTGTTGGCTNTTTAGANAAAAACACATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_18301196_18301475_oy-0_ler-0_645763_747205_TRUE
SEQUENCE_TARGET=950,379
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_15092544_15092715_hi-0_mt-0_182923_19659_TRUE
SEQUENCE_TARGET=950,271
SEQUENCE_TEMPLATE=CNACAGTTCCAACTATNTNCAGAGTTCTAACCNAAACAGAAACTNTCTGNNANATACGAAATGATTCANTTTNAGAAATTACGAAACCTGGAAGATGGNAATTGTGAAAGCTTCGTAGCAAATGGGACANTCGAGAATCTCCAGATCCATTAGCATNGTCGAGCGTTTCTTAGCGTCTCTCTTNGCTGCATCACTAGAAGAAAGTTGTCTCTTTTGCGAGAGAATGCTGTNNNNNNNNNNNNNNNNNNCGGNAGNTTCCAAAATCGCGGCANCAACCATGTCGGAGAGATGACTAAACCNTAAATTCGCTTTTANNNTTNTCGNAAACTGAGAGAGGNAANTAGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTTACAGAGAAANNAGNCAATGGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAGGAAATGATTNTTTGAAAGATTCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGATNTATAGGTTAGTATATGCTTAAACCAATATAATTTCAATAACCATANCGTTTNCAGAATTAANAATTTATGNGTTANNTAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNTNTTAGCATATTCATAGAGCATTCACCCNAATNATGAATTTGTGTNNNNNNNNNANTTTTTNAATTGTTANTTTTAATAANTAAAAATANNATAANNANTTGAATNCTNTTTTGTNCAAANAATNTNNNNNNNNTATTTNTNTNNNNAAACNNNAAATNNTGCTATTTATNNNAATTTCTNTTTTAANTTTTGCTATTATTTTTTCTNCTAAAGATTTAAGAATTTTGNTATTANNTTTTANATTNTTAACTAATATNTNAAATTGCAGTTAAAANTANCNTNTTNNAATTCTTTAATCATTGNATCACAATTCACAANTAANTTTTTTCTTCTGNTTTTAGACATCACACGTTCGTACGTTTACTTNNAATTAGATTTCNACTAAGAGNANNNNNNNNNNNGGNNNNNNTNATCATGACAAACGANAGAGCACGCATTACTCCAATTTGNNNNTNNANANNGTATNNNTNAGAAGTNNTNAGNNNAAAAAACTGTGAAATGTTTTGTTGGTCGATTCCATCNAAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_22442457_22443184_wil-2_bur-0_14887_23628_TRUE
SEQUENCE_TARGET=950,827
SEQUENCE_TEMPLATE=TCGTCGTTTTCTTTAAATCTCTNATGATCAATCTAATTAGCCCTTTTGNTNGTGGTCAGGATTTAAAGAAAGTTGTTTCTATCACTTGAAAATTCTCCACTAACTTGATGAGAATATGTGTGATGGAATCGATCTAGCGAACATATACTAATCNAANNNNNNNGCNTAAAATGGGACCCTCTTTGCACNAANNAAGTTTCAAGATATTATTATACCCACAAAACCAAATCTTCTTTAATCATGTCTAAATCATTTTAGGCACTCNTAACAAAANAATAATGTTATCNTCCAATTTGTCTACACTTTGGTATTGATTCGTTACAAGTGAATGTTCTAGACCACGTTTGTTGATCTATTATGTAGTTTTGTATTCATCCTAGCTACATTNNTATATATCCACTTATTTCTTAACTACGGTATGCATTATACATGTTTGTATTAATGTCGCCGATAAAANTTTCGGNCCCATAGTGATGANTAATCATTCTCGATTATTATTGTNAAANAAAACACTACAAGGAATNAGATAGATCAAAAACGAAAGCTCCNTNGAAAACAATCTCAAAGAACAANAAAATATGAGAAGACTCTTTCTCTTCTCTCTCCTCTTTTTATTCTTTTACTCATCCTCCTCCTCAAGATCTTCATCAGAATCTCACATCTTCATNTACGGAGGATGTTCACCGGAAAAATACACACCAAACACACCTTTTGAGTCAAACCGCGACACTTTCCTCTCCTCTGTCGTNACTTCCTCCTCTGACGCGTCCTTCAACAGCTTTGCCGTCGGCAACGACTCCTCCTCCTCCTCNTCCTCTTCCGCCGTCTTCGGCCTCTATCAATGCCGTGACGATCTCCNATCATCAGACTGCTCAAAATGCATCCAAACCTCAGTCGATCAAATCACTCTCATTTGTCCTTACTCTTACGGCGCTTCTCTCCAGCTCGAAGGCTGCTTCTTACGTTACGAAACAAACGATTTTCTAGGGAAACNAGACACNAGTCTTAGGTACAAGAAGTGTAGTTCCAAGAGCGTTGAGAATGACTACGATTTCTTTAAGCGGAGAGACGATGTGTTGTCGGATCTTGAGTCGACTCAACTCGGTTACAAAGTTAGCCGGTCCGGTTTAGTCGAAGGTTATGCTCAGTGTGTTGGTGACTTGAGTCCTAGTGACTGTACGGCTTGTCTTGCCGAGTCTGTCGGAAAATTAAAGAATCTTTGTGGCTCGGCGGTTGCAGCTGAGGTTTACTTGGCTCAGTGCTATGCTCGTTATTGGGGCTCTGGTTACTATGACTTCTCTTCAGGTCAGTCCATAAATTAAAATCTTTATTTCATTTANTAGCTATTGCTCTAATTTACGTAAGTTCTTTAATTANATTCGTGAATNATGNTTAGTGCCATGATNATATAATTGAAAANTGTAGTAATGAACTGTATAGAAATGTGTGAGAATTTGACAAAATTTAAATTTCCGCCGCTTATAATTGAGAAAATCACAAGTCTAGTCGCTCCTTNGTAGCACGAGAAAGTTGAAGAAATCATAAAATTTTCGTTGNTTTTTTTTTTTNGTAATTTCNANTGGAAAGACACCATTAAGTATTGAATAAAATAGAATTAATCTTTTGAATCATTCGTTGGAAAATTCGAGTATCATGATTATAATATCATTGGTCAGAAAAGAATGTTTTTGCAATATATTATTCNTTTCTTTATAGGCATAGCCACCNATGTCCCCATNTTCAACTTTATTTTATATATTCTTTATAGNTTNATCAATATCACATAGTCCAAAAGTCATTGCATCTTAGAAAATCTAATTCTTNNNNNNATACTANNNTGTCCTTATCTTATGTACTTTAATTTGTCACATACAATANTAAGATCTTTTTAAATTTGATCTAATTTTCGTTATTAAGATCCAACGAATGGAGATCACGTGGGGAAATCAATCGCGATCATCGTAGGAGTCATTGCTGGNTTCGCAATTCTTGTTGTTCTCCTCTCCCTTTGTAGAAACTCCATGCGTAAGTCTATACTTACTTGCCTAATNTTCACATGATTAAGATTTAAGATCAAATCACTCATTTTTAGTATATATTTAGAAATAAGTATCATGATCTNATAATTTTTCTTATCTGATTTGCAGATTGATATAATGAAAGGCATGATTCGGGATACAAGATTCTAGGGACCTNTTTTTTTTTTTTTNAANTCTCTTGTATTCCACTATTCCTTTTATTTTGTAGGAGGAACACATCCGTTACCGTTCCTTGCTGAAATTTTTGGTTTATCAAAAGGAAAACTATTAGTGNCTGATNATATAATNANNTTTAGCTTTGAGGTATTAAATATTTGTATAGTGTGACCCNTATTGTCTGCCTTAAAAAGATGCTAACTTTATTCCATTAACTAGTTTTAGACTAAAATCCACTTTCCTTAGCAGGCGCAATTATTCGATACTCATACTTAGATAAGTAGATTATCATTTCCGGAGAACAACGTGATTCGATCGATTGAGTATCATTNAACTTTAAAAGGTTCTGCCGGCAAAAAAAAANAAGAAGAAGGCTTATAAGACTTGAGAGGTTCCATTCAATCAATTCTTTGGTTTTCTACGATCCGGATCTACCCGGTTCTCATGGGTTTCACCACATATTCCATTGAGCCATTAAATTATTATGGACTGGGCCTCGGCCTACTAAGCTTCTAAGCCGTGTAACATATCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.887040
PRIMER_LEFT_0_PENALTY=0.190632
PRIMER_RIGHT_0_PENALTY=4.696408
PRIMER_LEFT_0_SEQUENCE=CTGCTCAAAATGCATCCAAA
PRIMER_RIGHT_0_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_0=868,20
PRIMER_RIGHT_0=1801,22
PRIMER_LEFT_0_TM=59.809
PRIMER_RIGHT_0_TM=57.304
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.8000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=934
PRIMER_PAIR_1_PENALTY=5.026196
PRIMER_LEFT_1_PENALTY=0.329789
PRIMER_RIGHT_1_PENALTY=4.696408
PRIMER_LEFT_1_SEQUENCE=TGCTCAAAATGCATCCAAAC
PRIMER_RIGHT_1_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_1=869,20
PRIMER_RIGHT_1=1801,22
PRIMER_LEFT_1_TM=59.670
PRIMER_RIGHT_1_TM=57.304
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=933
PRIMER_PAIR_2_PENALTY=5.153005
PRIMER_LEFT_2_PENALTY=0.456597
PRIMER_RIGHT_2_PENALTY=4.696408
PRIMER_LEFT_2_SEQUENCE=GCTCAAAATGCATCCAAACC
PRIMER_RIGHT_2_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_2=870,20
PRIMER_RIGHT_2=1801,22
PRIMER_LEFT_2_TM=60.457
PRIMER_RIGHT_2_TM=57.304
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=932
PRIMER_PAIR_3_PENALTY=5.265479
PRIMER_LEFT_3_PENALTY=0.456597
PRIMER_RIGHT_3_PENALTY=4.808882
PRIMER_LEFT_3_SEQUENCE=GCTCAAAATGCATCCAAACC
PRIMER_RIGHT_3_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_3=870,20
PRIMER_RIGHT_3=1804,20
PRIMER_LEFT_3_TM=60.457
PRIMER_RIGHT_3_TM=55.191
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=935
PRIMER_PAIR_4_PENALTY=5.265479
PRIMER_LEFT_4_PENALTY=0.456597
PRIMER_RIGHT_4_PENALTY=4.808882
PRIMER_LEFT_4_SEQUENCE=GCTCAAAATGCATCCAAACC
PRIMER_RIGHT_4_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_4=870,20
PRIMER_RIGHT_4=1805,20
PRIMER_LEFT_4_TM=60.457
PRIMER_RIGHT_4_TM=55.191
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=936
PRIMER_PAIR_5_PENALTY=5.562071
PRIMER_LEFT_5_PENALTY=0.865663
PRIMER_RIGHT_5_PENALTY=4.696408
PRIMER_LEFT_5_SEQUENCE=CCTTACTCTTACGGCGCTTC
PRIMER_RIGHT_5_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_5=917,20
PRIMER_RIGHT_5=1801,22
PRIMER_LEFT_5_TM=59.134
PRIMER_RIGHT_5_TM=57.304
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=885
PRIMER_PAIR_6_PENALTY=5.562071
PRIMER_LEFT_6_PENALTY=0.865663
PRIMER_RIGHT_6_PENALTY=4.696408
PRIMER_LEFT_6_SEQUENCE=TCCTTACTCTTACGGCGCTT
PRIMER_RIGHT_6_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_6=916,20
PRIMER_RIGHT_6=1801,22
PRIMER_LEFT_6_TM=59.134
PRIMER_RIGHT_6_TM=57.304
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.2000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=886
PRIMER_PAIR_7_PENALTY=5.674545
PRIMER_LEFT_7_PENALTY=0.865663
PRIMER_RIGHT_7_PENALTY=4.808882
PRIMER_LEFT_7_SEQUENCE=CCTTACTCTTACGGCGCTTC
PRIMER_RIGHT_7_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_7=917,20
PRIMER_RIGHT_7=1804,20
PRIMER_LEFT_7_TM=59.134
PRIMER_RIGHT_7_TM=55.191
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=888
PRIMER_PAIR_8_PENALTY=5.674545
PRIMER_LEFT_8_PENALTY=0.865663
PRIMER_RIGHT_8_PENALTY=4.808882
PRIMER_LEFT_8_SEQUENCE=CCTTACTCTTACGGCGCTTC
PRIMER_RIGHT_8_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_8=917,20
PRIMER_RIGHT_8=1805,20
PRIMER_LEFT_8_TM=59.134
PRIMER_RIGHT_8_TM=55.191
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=889
PRIMER_PAIR_9_PENALTY=5.674545
PRIMER_LEFT_9_PENALTY=0.865663
PRIMER_RIGHT_9_PENALTY=4.808882
PRIMER_LEFT_9_SEQUENCE=TCCTTACTCTTACGGCGCTT
PRIMER_RIGHT_9_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_9=916,20
PRIMER_RIGHT_9=1804,20
PRIMER_LEFT_9_TM=59.134
PRIMER_RIGHT_9_TM=55.191
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=10.2000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=889
PRIMER_PAIR_10_PENALTY=5.674545
PRIMER_LEFT_10_PENALTY=0.865663
PRIMER_RIGHT_10_PENALTY=4.808882
PRIMER_LEFT_10_SEQUENCE=TCCTTACTCTTACGGCGCTT
PRIMER_RIGHT_10_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_10=916,20
PRIMER_RIGHT_10=1805,20
PRIMER_LEFT_10_TM=59.134
PRIMER_RIGHT_10_TM=55.191
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=10.2000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=890
PRIMER_PAIR_11_PENALTY=5.744819
PRIMER_LEFT_11_PENALTY=1.048411
PRIMER_RIGHT_11_PENALTY=4.696408
PRIMER_LEFT_11_SEQUENCE=CCTTACTCTTACGGCGCTTCT
PRIMER_RIGHT_11_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_11=917,21
PRIMER_RIGHT_11=1801,22
PRIMER_LEFT_11_TM=60.048
PRIMER_RIGHT_11_TM=57.304
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=885
PRIMER_PAIR_12_PENALTY=5.770027
PRIMER_LEFT_12_PENALTY=1.073619
PRIMER_RIGHT_12_PENALTY=4.696408
PRIMER_LEFT_12_SEQUENCE=GTCCTTACTCTTACGGCGCTT
PRIMER_RIGHT_12_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_12=915,21
PRIMER_RIGHT_12=1801,22
PRIMER_LEFT_12_TM=59.926
PRIMER_RIGHT_12_TM=57.304
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.2000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=887
PRIMER_PAIR_13_PENALTY=5.854158
PRIMER_LEFT_13_PENALTY=1.157751
PRIMER_RIGHT_13_PENALTY=4.696408
PRIMER_LEFT_13_SEQUENCE=ACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_13_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_13=921,20
PRIMER_RIGHT_13=1801,22
PRIMER_LEFT_13_TM=58.842
PRIMER_RIGHT_13_TM=57.304
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=881
PRIMER_PAIR_14_PENALTY=5.857293
PRIMER_LEFT_14_PENALTY=1.048411
PRIMER_RIGHT_14_PENALTY=4.808882
PRIMER_LEFT_14_SEQUENCE=CCTTACTCTTACGGCGCTTCT
PRIMER_RIGHT_14_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_14=917,21
PRIMER_RIGHT_14=1804,20
PRIMER_LEFT_14_TM=60.048
PRIMER_RIGHT_14_TM=55.191
PRIMER_LEFT_14_GC_PERCENT=52.381
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=888
PRIMER_PAIR_15_PENALTY=5.857293
PRIMER_LEFT_15_PENALTY=1.048411
PRIMER_RIGHT_15_PENALTY=4.808882
PRIMER_LEFT_15_SEQUENCE=CCTTACTCTTACGGCGCTTCT
PRIMER_RIGHT_15_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_15=917,21
PRIMER_RIGHT_15=1805,20
PRIMER_LEFT_15_TM=60.048
PRIMER_RIGHT_15_TM=55.191
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=889
PRIMER_PAIR_16_PENALTY=5.882501
PRIMER_LEFT_16_PENALTY=1.073619
PRIMER_RIGHT_16_PENALTY=4.808882
PRIMER_LEFT_16_SEQUENCE=GTCCTTACTCTTACGGCGCTT
PRIMER_RIGHT_16_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_16=915,21
PRIMER_RIGHT_16=1804,20
PRIMER_LEFT_16_TM=59.926
PRIMER_RIGHT_16_TM=55.191
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=10.2000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=890
PRIMER_PAIR_17_PENALTY=5.882501
PRIMER_LEFT_17_PENALTY=1.073619
PRIMER_RIGHT_17_PENALTY=4.808882
PRIMER_LEFT_17_SEQUENCE=GTCCTTACTCTTACGGCGCTT
PRIMER_RIGHT_17_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_17=915,21
PRIMER_RIGHT_17=1805,20
PRIMER_LEFT_17_TM=59.926
PRIMER_RIGHT_17_TM=55.191
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=10.2000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=891
PRIMER_PAIR_18_PENALTY=5.966633
PRIMER_LEFT_18_PENALTY=1.157751
PRIMER_RIGHT_18_PENALTY=4.808882
PRIMER_LEFT_18_SEQUENCE=ACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_18_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_18=921,20
PRIMER_RIGHT_18=1804,20
PRIMER_LEFT_18_TM=58.842
PRIMER_RIGHT_18_TM=55.191
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=884
PRIMER_PAIR_19_PENALTY=5.966633
PRIMER_LEFT_19_PENALTY=1.157751
PRIMER_RIGHT_19_PENALTY=4.808882
PRIMER_LEFT_19_SEQUENCE=ACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_19_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_19=921,20
PRIMER_RIGHT_19=1805,20
PRIMER_LEFT_19_TM=58.842
PRIMER_RIGHT_19_TM=55.191
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=885
PRIMER_PAIR_20_PENALTY=6.136345
PRIMER_LEFT_20_PENALTY=1.439937
PRIMER_RIGHT_20_PENALTY=4.696408
PRIMER_LEFT_20_SEQUENCE=CTCAAAATGCATCCAAACCTC
PRIMER_RIGHT_20_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_20=871,21
PRIMER_RIGHT_20=1801,22
PRIMER_LEFT_20_TM=59.560
PRIMER_RIGHT_20_TM=57.304
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=931
PRIMER_PAIR_21_PENALTY=6.149414
PRIMER_LEFT_21_PENALTY=1.453007
PRIMER_RIGHT_21_PENALTY=4.696408
PRIMER_LEFT_21_SEQUENCE=TCAAAATGCATCCAAACCTC
PRIMER_RIGHT_21_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_21=872,20
PRIMER_RIGHT_21=1801,22
PRIMER_LEFT_21_TM=58.547
PRIMER_RIGHT_21_TM=57.304
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.6000
PRIMER_RIGHT_21_END_STABILITY=6.6000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=930
PRIMER_PAIR_22_PENALTY=6.234987
PRIMER_LEFT_22_PENALTY=1.538580
PRIMER_RIGHT_22_PENALTY=4.696408
PRIMER_LEFT_22_SEQUENCE=CTCTTACGGCGCTTCTCTCC
PRIMER_RIGHT_22_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_22=922,20
PRIMER_RIGHT_22=1801,22
PRIMER_LEFT_22_TM=61.539
PRIMER_RIGHT_22_TM=57.304
PRIMER_LEFT_22_GC_PERCENT=60.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=880
PRIMER_PAIR_23_PENALTY=6.328344
PRIMER_LEFT_23_PENALTY=1.631936
PRIMER_RIGHT_23_PENALTY=4.696408
PRIMER_LEFT_23_SEQUENCE=CTGCTCAAAATGCATCCAAAC
PRIMER_RIGHT_23_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_23=868,21
PRIMER_RIGHT_23=1801,22
PRIMER_LEFT_23_TM=60.632
PRIMER_RIGHT_23_TM=57.304
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=934
PRIMER_PAIR_24_PENALTY=6.347462
PRIMER_LEFT_24_PENALTY=1.538580
PRIMER_RIGHT_24_PENALTY=4.808882
PRIMER_LEFT_24_SEQUENCE=CTCTTACGGCGCTTCTCTCC
PRIMER_RIGHT_24_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_24=922,20
PRIMER_RIGHT_24=1804,20
PRIMER_LEFT_24_TM=61.539
PRIMER_RIGHT_24_TM=55.191
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=883
PRIMER_PAIR_25_PENALTY=6.347462
PRIMER_LEFT_25_PENALTY=1.538580
PRIMER_RIGHT_25_PENALTY=4.808882
PRIMER_LEFT_25_SEQUENCE=CTCTTACGGCGCTTCTCTCC
PRIMER_RIGHT_25_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_25=922,20
PRIMER_RIGHT_25=1805,20
PRIMER_LEFT_25_TM=61.539
PRIMER_RIGHT_25_TM=55.191
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=884
PRIMER_PAIR_26_PENALTY=6.568908
PRIMER_LEFT_26_PENALTY=1.872501
PRIMER_RIGHT_26_PENALTY=4.696408
PRIMER_LEFT_26_SEQUENCE=TCCTTACTCTTACGGCGCTTC
PRIMER_RIGHT_26_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_26=916,21
PRIMER_RIGHT_26=1801,22
PRIMER_LEFT_26_TM=60.873
PRIMER_RIGHT_26_TM=57.304
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=886
PRIMER_PAIR_27_PENALTY=6.681383
PRIMER_LEFT_27_PENALTY=1.872501
PRIMER_RIGHT_27_PENALTY=4.808882
PRIMER_LEFT_27_SEQUENCE=TCCTTACTCTTACGGCGCTTC
PRIMER_RIGHT_27_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_27=916,21
PRIMER_RIGHT_27=1804,20
PRIMER_LEFT_27_TM=60.873
PRIMER_RIGHT_27_TM=55.191
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=889
PRIMER_PAIR_28_PENALTY=6.681383
PRIMER_LEFT_28_PENALTY=1.872501
PRIMER_RIGHT_28_PENALTY=4.808882
PRIMER_LEFT_28_SEQUENCE=TCCTTACTCTTACGGCGCTTC
PRIMER_RIGHT_28_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_28=916,21
PRIMER_RIGHT_28=1805,20
PRIMER_LEFT_28_TM=60.873
PRIMER_RIGHT_28_TM=55.191
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=890
PRIMER_PAIR_29_PENALTY=6.800893
PRIMER_LEFT_29_PENALTY=2.104485
PRIMER_RIGHT_29_PENALTY=4.696408
PRIMER_LEFT_29_SEQUENCE=TACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_29_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_29=920,21
PRIMER_RIGHT_29=1801,22
PRIMER_LEFT_29_TM=58.896
PRIMER_RIGHT_29_TM=57.304
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=882
PRIMER_PAIR_30_PENALTY=6.865582
PRIMER_LEFT_30_PENALTY=2.169174
PRIMER_RIGHT_30_PENALTY=4.696408
PRIMER_LEFT_30_SEQUENCE=TTACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_30_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_30=919,22
PRIMER_RIGHT_30=1801,22
PRIMER_LEFT_30_TM=60.169
PRIMER_RIGHT_30_TM=57.304
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=883
PRIMER_PAIR_31_PENALTY=6.913368
PRIMER_LEFT_31_PENALTY=2.104485
PRIMER_RIGHT_31_PENALTY=4.808882
PRIMER_LEFT_31_SEQUENCE=TACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_31_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_31=920,21
PRIMER_RIGHT_31=1804,20
PRIMER_LEFT_31_TM=58.896
PRIMER_RIGHT_31_TM=55.191
PRIMER_LEFT_31_GC_PERCENT=52.381
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=885
PRIMER_PAIR_32_PENALTY=6.913368
PRIMER_LEFT_32_PENALTY=2.104485
PRIMER_RIGHT_32_PENALTY=4.808882
PRIMER_LEFT_32_SEQUENCE=TACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_32_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_32=920,21
PRIMER_RIGHT_32=1805,20
PRIMER_LEFT_32_TM=58.896
PRIMER_RIGHT_32_TM=55.191
PRIMER_LEFT_32_GC_PERCENT=52.381
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=886
PRIMER_PAIR_33_PENALTY=6.978056
PRIMER_LEFT_33_PENALTY=2.169174
PRIMER_RIGHT_33_PENALTY=4.808882
PRIMER_LEFT_33_SEQUENCE=TTACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_33_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_33=919,22
PRIMER_RIGHT_33=1804,20
PRIMER_LEFT_33_TM=60.169
PRIMER_RIGHT_33_TM=55.191
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=7.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=886
PRIMER_PAIR_34_PENALTY=6.978056
PRIMER_LEFT_34_PENALTY=2.169174
PRIMER_RIGHT_34_PENALTY=4.808882
PRIMER_LEFT_34_SEQUENCE=TTACTCTTACGGCGCTTCTCTC
PRIMER_RIGHT_34_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_34=919,22
PRIMER_RIGHT_34=1805,20
PRIMER_LEFT_34_TM=60.169
PRIMER_RIGHT_34_TM=55.191
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=887
PRIMER_PAIR_35_PENALTY=7.026516
PRIMER_LEFT_35_PENALTY=2.330108
PRIMER_RIGHT_35_PENALTY=4.696408
PRIMER_LEFT_35_SEQUENCE=CTCAAAATGCATCCAAACCT
PRIMER_RIGHT_35_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_35=871,20
PRIMER_RIGHT_35=1801,22
PRIMER_LEFT_35_TM=57.670
PRIMER_RIGHT_35_TM=57.304
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.6000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=931
PRIMER_PAIR_36_PENALTY=7.050369
PRIMER_LEFT_36_PENALTY=2.353961
PRIMER_RIGHT_36_PENALTY=4.696408
PRIMER_LEFT_36_SEQUENCE=GCTCAAAATGCATCCAAACCT
PRIMER_RIGHT_36_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_36=870,21
PRIMER_RIGHT_36=1801,22
PRIMER_LEFT_36_TM=61.354
PRIMER_RIGHT_36_TM=57.304
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=6.6000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=932
PRIMER_PAIR_37_PENALTY=7.056728
PRIMER_LEFT_37_PENALTY=2.360320
PRIMER_RIGHT_37_PENALTY=4.696408
PRIMER_LEFT_37_SEQUENCE=AAAATGCATCCAAACCTCAGTC
PRIMER_RIGHT_37_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_37=874,22
PRIMER_RIGHT_37=1801,22
PRIMER_LEFT_37_TM=60.360
PRIMER_RIGHT_37_TM=57.304
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=7.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=928
PRIMER_PAIR_38_PENALTY=7.112483
PRIMER_LEFT_38_PENALTY=0.190632
PRIMER_RIGHT_38_PENALTY=6.921851
PRIMER_LEFT_38_SEQUENCE=CTGCTCAAAATGCATCCAAA
PRIMER_RIGHT_38_SEQUENCE=GATGCAATGACTTTTGGACTATGTG
PRIMER_LEFT_38=868,20
PRIMER_RIGHT_38=1804,25
PRIMER_LEFT_38_TM=59.809
PRIMER_RIGHT_38_TM=61.922
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.8000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=937
PRIMER_PAIR_39_PENALTY=7.119122
PRIMER_LEFT_39_PENALTY=2.422714
PRIMER_RIGHT_39_PENALTY=4.696408
PRIMER_LEFT_39_SEQUENCE=AATGCATCCAAACCTCAGTC
PRIMER_RIGHT_39_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_39=876,20
PRIMER_RIGHT_39=1801,22
PRIMER_LEFT_39_TM=57.577
PRIMER_RIGHT_39_TM=57.304
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=7.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=926
PRIMER_PAIR_40_PENALTY=7.238005
PRIMER_LEFT_40_PENALTY=2.541598
PRIMER_RIGHT_40_PENALTY=4.696408
PRIMER_LEFT_40_SEQUENCE=CCGTCTTCGGCCTCTATCAA
PRIMER_RIGHT_40_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_40=822,20
PRIMER_RIGHT_40=1801,22
PRIMER_LEFT_40_TM=62.542
PRIMER_RIGHT_40_TM=57.304
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=980
PRIMER_PAIR_41_PENALTY=7.251640
PRIMER_LEFT_41_PENALTY=0.329789
PRIMER_RIGHT_41_PENALTY=6.921851
PRIMER_LEFT_41_SEQUENCE=TGCTCAAAATGCATCCAAAC
PRIMER_RIGHT_41_SEQUENCE=GATGCAATGACTTTTGGACTATGTG
PRIMER_LEFT_41=869,20
PRIMER_RIGHT_41=1804,25
PRIMER_LEFT_41_TM=59.670
PRIMER_RIGHT_41_TM=61.922
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=6.6000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=936
PRIMER_PAIR_42_PENALTY=7.257078
PRIMER_LEFT_42_PENALTY=2.560670
PRIMER_RIGHT_42_PENALTY=4.696408
PRIMER_LEFT_42_SEQUENCE=TTACTCTTACGGCGCTTCTCT
PRIMER_RIGHT_42_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_42=919,21
PRIMER_RIGHT_42=1801,22
PRIMER_LEFT_42_TM=58.439
PRIMER_RIGHT_42_TM=57.304
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=883
PRIMER_PAIR_43_PENALTY=7.257078
PRIMER_LEFT_43_PENALTY=2.560670
PRIMER_RIGHT_43_PENALTY=4.696408
PRIMER_LEFT_43_SEQUENCE=CTTACTCTTACGGCGCTTCTC
PRIMER_RIGHT_43_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_43=918,21
PRIMER_RIGHT_43=1801,22
PRIMER_LEFT_43_TM=58.439
PRIMER_RIGHT_43_TM=57.304
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=884
PRIMER_PAIR_44_PENALTY=7.272893
PRIMER_LEFT_44_PENALTY=2.576485
PRIMER_RIGHT_44_PENALTY=4.696408
PRIMER_LEFT_44_SEQUENCE=TTACTCTTACGGCGCTTCTC
PRIMER_RIGHT_44_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_44=919,20
PRIMER_RIGHT_44=1801,22
PRIMER_LEFT_44_TM=57.424
PRIMER_RIGHT_44_TM=57.304
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=883
PRIMER_PAIR_45_PENALTY=7.327811
PRIMER_LEFT_45_PENALTY=2.631404
PRIMER_RIGHT_45_PENALTY=4.696408
PRIMER_LEFT_45_SEQUENCE=CTTACTCTTACGGCGCTTCTCT
PRIMER_RIGHT_45_SEQUENCE=GCAATGACTTTTGGACTATGTG
PRIMER_LEFT_45=918,22
PRIMER_RIGHT_45=1801,22
PRIMER_LEFT_45_TM=59.369
PRIMER_RIGHT_45_TM=57.304
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.6000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=884
PRIMER_PAIR_46_PENALTY=7.350480
PRIMER_LEFT_46_PENALTY=2.541598
PRIMER_RIGHT_46_PENALTY=4.808882
PRIMER_LEFT_46_SEQUENCE=CCGTCTTCGGCCTCTATCAA
PRIMER_RIGHT_46_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_46=822,20
PRIMER_RIGHT_46=1804,20
PRIMER_LEFT_46_TM=62.542
PRIMER_RIGHT_46_TM=55.191
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=983
PRIMER_PAIR_47_PENALTY=7.350480
PRIMER_LEFT_47_PENALTY=2.541598
PRIMER_RIGHT_47_PENALTY=4.808882
PRIMER_LEFT_47_SEQUENCE=CCGTCTTCGGCCTCTATCAA
PRIMER_RIGHT_47_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_47=822,20
PRIMER_RIGHT_47=1805,20
PRIMER_LEFT_47_TM=62.542
PRIMER_RIGHT_47_TM=55.191
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=984
PRIMER_PAIR_48_PENALTY=7.369552
PRIMER_LEFT_48_PENALTY=2.560670
PRIMER_RIGHT_48_PENALTY=4.808882
PRIMER_LEFT_48_SEQUENCE=TTACTCTTACGGCGCTTCTCT
PRIMER_RIGHT_48_SEQUENCE=GATGCAATGACTTTTGGACT
PRIMER_LEFT_48=919,21
PRIMER_RIGHT_48=1804,20
PRIMER_LEFT_48_TM=58.439
PRIMER_RIGHT_48_TM=55.191
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=886
PRIMER_PAIR_49_PENALTY=7.369552
PRIMER_LEFT_49_PENALTY=2.560670
PRIMER_RIGHT_49_PENALTY=4.808882
PRIMER_LEFT_49_SEQUENCE=TTACTCTTACGGCGCTTCTCT
PRIMER_RIGHT_49_SEQUENCE=AGATGCAATGACTTTTGGAC
PRIMER_LEFT_49=919,21
PRIMER_RIGHT_49=1805,20
PRIMER_LEFT_49_TM=58.439
PRIMER_RIGHT_49_TM=55.191
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=887
=
SEQUENCE_ID=MAGIC.329_4_4358917_4359176_ws-0_ler-0_30629_83415_TRUE
SEQUENCE_TARGET=950,359
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_15163410_15163476_no-0_zu-0_22624_50488_TRUE
SEQUENCE_TARGET=950,166
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_13615977_13616041_ct-1_tsu-0_841268_275044_TRUE
SEQUENCE_TARGET=950,164
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_12771597_12771741_ct-1_no-0_814791_2996_TRUE
SEQUENCE_TARGET=950,244
SEQUENCE_TEMPLATE=AGGCGAGAGGGTTATGTTTCNGCTGCAANNAACCNNNNNTATTCCCCTCAANATGATTGCCANTANAAGTCTCTTAGAGCATCCTCAANGGGAGGATTTAAGATNTTTNTTAACTAAATAAATNATTAAAATTAANTCCAAAATAATGACTTTAGGTTAGANGCGCTCTTAGCNTAGAGGCGCTCTTAGGTAATTGCAATGGTNATNTGGCATTGTTTGATGTCTTTACNTNNCTNCTNTCTCTCTCTCTCTCTNNNNNNNNNNNNGTCTCCCTCNCACCGGAGATGGATTCTTCANTATCTTCTCCTCAATCACAAATCCTTTATTGATCANACTATTCANNNNNNNNNNNNTACACGCTGCTCCTCTTCTTTTCCTTAGATTCCATGGCTTCACNNGAGAAATNNNNTCCTTGATAGTTAAGAACTGGAGATCTCTGTTATATCGATGAAGATGGATTCATATTTGTGGTTAATCGGTTAAANGAACTGATCAAGTACTATGTTAACATAGATTGTTACTTTAATTNCTTTAGTAAAGATTTATACTGGTTTTGGATGTATACTTTGTGANTNAAGNGAGANAANNNANTTTACAACTACNGTANNTGATTGATTTTTTAAGAACTCCANCTTANTATATTATCATTGNCAGANGNGTATCAAGGANTTTTTTTACCCAGTTCAAAACGTAACAAATAAAATGTCTTTAATNNAAAAAAATCNTTTTTTTACAACTTCTCAAACNAAAANAAACGTCCTACAAAAGCATCCTACGATCCTTTATGTCNTGNAATCTCTTTATCACAATCTCATTTGTAATANTATTGAATACATCTTTCTCAATAAAACAAACTAAACAATCATTCAAAAATTNNTCTCCAATACAATTTCGTCGATGTGTCTTTACGATCNTCATGGCTGAAAAACATCTCTCAACAGTAGCANNAGCAACANGCAAAATCAAAACCAATTTCAAAAGCCTATAACCAAANGGATATNACAAANGTTTTCGTGTTTCCACCANCTTCTGANCAATGTCTCCAAGATTCTTTAAATTAGAAAATCTCTCATCATTTCGAANAGTATCAATGTAGATAGATAATTNTTNCTCAATAGATATGAACTCTCCTAAACTAAAGTNANCTTGGTAGAACTTAGATAACCTCACNAACTTTGAGTGATCAAACTCANAAAATGCATCAATGGGAGNTAAAGAAGCANCACAAATAAGCAATTCAGTGTTCACCTCATTAAAANGGTCGTCAAATTTCTGAAGNTNGAAATCCAAAACANAATATAAGCAATTGACCTTGTAGTGATGCATATTTGATACTCTTNTTTTGTTTCTTCGTCTCTTTGAGTCAACAAAATCTTCCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAAACNAANAAACTTTCANNATGAGAGAATCTCACCCATCATCCCTAAAACACTGAAGTTGTCTCTTAGTTGATTTCACCAATGANATNGCATTNAAAATATNNTGATCTCTTCTTTNGAGNNCCATAGACAAGTTCTNTGTGAGCNCTANAANGTGTATCATCATGTGNNNNNNNNNNAANNNNTCAAAGNTATGAAAGNANNTNAGGAGACTATGTGCTTGNCGTCTNNNNNNGTNATCTANGCCGTAATTCTCAAAGCATTCNAGNACCTTGATGATAGAANAGTACANCTNNNNNNNNNNNNNNNNNNNNNNNNNNTGAGAATTCNAACGAGTANNTNCTGNTCNTTGANGAGANANCTCTTGATTNAACCNTNTTNTAGTACTGANTTCANCNNNNATNNNNCCNNCTNNNNNNNNNNNNNNNNNNNNNNNTCGGATCAAATNTTTCCNCTTGCATGAAGCTCNAACNACATTTAACAACAAAGAAATCANATNNAAAAAGTCTCCAACATTNANATGNTTTTNTNCAACTNNNNNNACAACTAANTGAANNTNNNNNNNNNNNCAATGAACATAANATGNTGATGTGNTNTCCTTCAAAATTAATGANTNNNNNNNNNNNNNNNNNNNNTTCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.420442
PRIMER_LEFT_0_PENALTY=2.243575
PRIMER_RIGHT_0_PENALTY=1.176867
PRIMER_LEFT_0_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_0_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_0=880,22
PRIMER_RIGHT_0=1463,21
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=60.177
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=584
PRIMER_PAIR_1_PENALTY=3.870922
PRIMER_LEFT_1_PENALTY=2.243575
PRIMER_RIGHT_1_PENALTY=1.627348
PRIMER_LEFT_1_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_1_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_1=880,22
PRIMER_RIGHT_1=1461,21
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=59.373
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=582
PRIMER_PAIR_2_PENALTY=3.945515
PRIMER_LEFT_2_PENALTY=2.243575
PRIMER_RIGHT_2_PENALTY=1.701940
PRIMER_LEFT_2_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_2_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_2=880,22
PRIMER_RIGHT_2=1466,21
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=59.298
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=587
PRIMER_PAIR_3_PENALTY=3.973346
PRIMER_LEFT_3_PENALTY=2.243575
PRIMER_RIGHT_3_PENALTY=1.729772
PRIMER_LEFT_3_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_3_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_3=880,22
PRIMER_RIGHT_3=1463,20
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=58.270
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=584
PRIMER_PAIR_4_PENALTY=4.083366
PRIMER_LEFT_4_PENALTY=2.243575
PRIMER_RIGHT_4_PENALTY=1.839792
PRIMER_LEFT_4_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_4_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_4=880,22
PRIMER_RIGHT_4=1464,21
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=59.160
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=585
PRIMER_PAIR_5_PENALTY=4.310198
PRIMER_LEFT_5_PENALTY=2.243575
PRIMER_RIGHT_5_PENALTY=2.066624
PRIMER_LEFT_5_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_5_SEQUENCE=TTTAGGGATGATGGGTGAGA
PRIMER_LEFT_5=880,22
PRIMER_RIGHT_5=1460,20
PRIMER_LEFT_5_TM=60.244
PRIMER_RIGHT_5_TM=57.933
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=581
PRIMER_PAIR_6_PENALTY=4.353085
PRIMER_LEFT_6_PENALTY=2.243575
PRIMER_RIGHT_6_PENALTY=2.109510
PRIMER_LEFT_6_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_6_SEQUENCE=AGTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_6=880,22
PRIMER_RIGHT_6=1465,22
PRIMER_LEFT_6_TM=60.244
PRIMER_RIGHT_6_TM=60.110
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=586
PRIMER_PAIR_7_PENALTY=4.353085
PRIMER_LEFT_7_PENALTY=2.243575
PRIMER_RIGHT_7_PENALTY=2.109510
PRIMER_LEFT_7_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_7_SEQUENCE=CAGTGTTTTAGGGATGATGGGT
PRIMER_LEFT_7=880,22
PRIMER_RIGHT_7=1466,22
PRIMER_LEFT_7_TM=60.244
PRIMER_RIGHT_7_TM=60.110
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=587
PRIMER_PAIR_8_PENALTY=4.430523
PRIMER_LEFT_8_PENALTY=2.243575
PRIMER_RIGHT_8_PENALTY=2.186949
PRIMER_LEFT_8_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_8_SEQUENCE=GTTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_8=880,22
PRIMER_RIGHT_8=1462,22
PRIMER_LEFT_8_TM=60.244
PRIMER_RIGHT_8_TM=60.187
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=583
PRIMER_PAIR_9_PENALTY=4.577644
PRIMER_LEFT_9_PENALTY=2.243575
PRIMER_RIGHT_9_PENALTY=2.334070
PRIMER_LEFT_9_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_9_SEQUENCE=TTTAGGGATGATGGGTGAGATT
PRIMER_LEFT_9=880,22
PRIMER_RIGHT_9=1460,22
PRIMER_LEFT_9_TM=60.244
PRIMER_RIGHT_9_TM=59.666
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=581
PRIMER_PAIR_10_PENALTY=4.784661
PRIMER_LEFT_10_PENALTY=2.243575
PRIMER_RIGHT_10_PENALTY=2.541086
PRIMER_LEFT_10_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_10_SEQUENCE=TTTTAGGGATGATGGGTGAG
PRIMER_LEFT_10=880,22
PRIMER_RIGHT_10=1461,20
PRIMER_LEFT_10_TM=60.244
PRIMER_RIGHT_10_TM=57.459
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=582
PRIMER_PAIR_11_PENALTY=4.871363
PRIMER_LEFT_11_PENALTY=2.243575
PRIMER_RIGHT_11_PENALTY=2.627789
PRIMER_LEFT_11_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_11_SEQUENCE=GTTTTAGGGATGATGGGTGAG
PRIMER_LEFT_11=880,22
PRIMER_RIGHT_11=1462,21
PRIMER_LEFT_11_TM=60.244
PRIMER_RIGHT_11_TM=58.372
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=583
PRIMER_PAIR_12_PENALTY=4.923077
PRIMER_LEFT_12_PENALTY=2.243575
PRIMER_RIGHT_12_PENALTY=2.679503
PRIMER_LEFT_12_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_12_SEQUENCE=TTAGGGATGATGGGTGAGATT
PRIMER_LEFT_12=880,22
PRIMER_RIGHT_12=1459,21
PRIMER_LEFT_12_TM=60.244
PRIMER_RIGHT_12_TM=58.320
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=580
PRIMER_PAIR_13_PENALTY=4.927528
PRIMER_LEFT_13_PENALTY=3.750661
PRIMER_RIGHT_13_PENALTY=1.176867
PRIMER_LEFT_13_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_13_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_13=677,20
PRIMER_RIGHT_13=1463,21
PRIMER_LEFT_13_TM=56.249
PRIMER_RIGHT_13_TM=60.177
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=787
PRIMER_PAIR_14_PENALTY=4.933843
PRIMER_LEFT_14_PENALTY=3.756976
PRIMER_RIGHT_14_PENALTY=1.176867
PRIMER_LEFT_14_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_14_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_14=914,20
PRIMER_RIGHT_14=1463,21
PRIMER_LEFT_14_TM=56.243
PRIMER_RIGHT_14_TM=60.177
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.3000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=550
PRIMER_PAIR_15_PENALTY=4.943091
PRIMER_LEFT_15_PENALTY=2.243575
PRIMER_RIGHT_15_PENALTY=2.699517
PRIMER_LEFT_15_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_15_SEQUENCE=GTTTTAGGGATGATGGGTGA
PRIMER_LEFT_15=880,22
PRIMER_RIGHT_15=1462,20
PRIMER_LEFT_15_TM=60.244
PRIMER_RIGHT_15_TM=57.300
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=583
PRIMER_PAIR_16_PENALTY=4.990110
PRIMER_LEFT_16_PENALTY=3.813243
PRIMER_RIGHT_16_PENALTY=1.176867
PRIMER_LEFT_16_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_16_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_16=886,20
PRIMER_RIGHT_16=1463,21
PRIMER_LEFT_16_TM=56.187
PRIMER_RIGHT_16_TM=60.177
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=578
PRIMER_PAIR_17_PENALTY=5.211491
PRIMER_LEFT_17_PENALTY=2.243575
PRIMER_RIGHT_17_PENALTY=2.967916
PRIMER_LEFT_17_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_17_SEQUENCE=GTGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_17=880,22
PRIMER_RIGHT_17=1464,22
PRIMER_LEFT_17_TM=60.244
PRIMER_RIGHT_17_TM=60.968
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=585
PRIMER_PAIR_18_PENALTY=5.212823
PRIMER_LEFT_18_PENALTY=4.035956
PRIMER_RIGHT_18_PENALTY=1.176867
PRIMER_LEFT_18_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_18_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_18=752,24
PRIMER_RIGHT_18=1463,21
PRIMER_LEFT_18_TM=59.964
PRIMER_RIGHT_18_TM=60.177
PRIMER_LEFT_18_GC_PERCENT=41.667
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=712
PRIMER_PAIR_19_PENALTY=5.294586
PRIMER_LEFT_19_PENALTY=2.243575
PRIMER_RIGHT_19_PENALTY=3.051012
PRIMER_LEFT_19_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_19_SEQUENCE=TTCAGTGTTTTAGGGATGATGG
PRIMER_LEFT_19=880,22
PRIMER_RIGHT_19=1468,22
PRIMER_LEFT_19_TM=60.244
PRIMER_RIGHT_19_TM=58.949
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=589
PRIMER_PAIR_20_PENALTY=5.331478
PRIMER_LEFT_20_PENALTY=2.243575
PRIMER_RIGHT_20_PENALTY=3.087904
PRIMER_LEFT_20_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_20_SEQUENCE=TGTTTTAGGGATGATGGGTGAG
PRIMER_LEFT_20=880,22
PRIMER_RIGHT_20=1463,22
PRIMER_LEFT_20_TM=60.244
PRIMER_RIGHT_20_TM=61.088
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=584
PRIMER_PAIR_21_PENALTY=5.331478
PRIMER_LEFT_21_PENALTY=2.243575
PRIMER_RIGHT_21_PENALTY=3.087904
PRIMER_LEFT_21_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_21_SEQUENCE=TCAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_21=880,22
PRIMER_RIGHT_21=1467,22
PRIMER_LEFT_21_TM=60.244
PRIMER_RIGHT_21_TM=61.088
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=588
PRIMER_PAIR_22_PENALTY=5.364750
PRIMER_LEFT_22_PENALTY=2.243575
PRIMER_RIGHT_22_PENALTY=3.121175
PRIMER_LEFT_22_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_22_SEQUENCE=CTTCAGTGTTTTAGGGATGATGG
PRIMER_LEFT_22=880,22
PRIMER_RIGHT_22=1469,23
PRIMER_LEFT_22_TM=60.244
PRIMER_RIGHT_22_TM=59.879
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=43.478
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=590
PRIMER_PAIR_23_PENALTY=5.378008
PRIMER_LEFT_23_PENALTY=3.750661
PRIMER_RIGHT_23_PENALTY=1.627348
PRIMER_LEFT_23_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_23_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_23=677,20
PRIMER_RIGHT_23=1461,21
PRIMER_LEFT_23_TM=56.249
PRIMER_RIGHT_23_TM=59.373
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=785
PRIMER_PAIR_24_PENALTY=5.403939
PRIMER_LEFT_24_PENALTY=4.227072
PRIMER_RIGHT_24_PENALTY=1.176867
PRIMER_LEFT_24_SEQUENCE=AACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_24_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_24=753,23
PRIMER_RIGHT_24=1463,21
PRIMER_LEFT_24_TM=58.773
PRIMER_RIGHT_24_TM=60.177
PRIMER_LEFT_24_GC_PERCENT=43.478
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=711
PRIMER_PAIR_25_PENALTY=5.423173
PRIMER_LEFT_25_PENALTY=2.243575
PRIMER_RIGHT_25_PENALTY=3.179598
PRIMER_LEFT_25_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_25_SEQUENCE=TAGGGATGATGGGTGAGATT
PRIMER_LEFT_25=880,22
PRIMER_RIGHT_25=1458,20
PRIMER_LEFT_25_TM=60.244
PRIMER_RIGHT_25_TM=56.820
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=579
PRIMER_PAIR_26_PENALTY=5.440591
PRIMER_LEFT_26_PENALTY=3.813243
PRIMER_RIGHT_26_PENALTY=1.627348
PRIMER_LEFT_26_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_26_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_26=886,20
PRIMER_RIGHT_26=1461,21
PRIMER_LEFT_26_TM=56.187
PRIMER_RIGHT_26_TM=59.373
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=576
PRIMER_PAIR_27_PENALTY=5.452601
PRIMER_LEFT_27_PENALTY=3.750661
PRIMER_RIGHT_27_PENALTY=1.701940
PRIMER_LEFT_27_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_27_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_27=677,20
PRIMER_RIGHT_27=1466,21
PRIMER_LEFT_27_TM=56.249
PRIMER_RIGHT_27_TM=59.298
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=790
PRIMER_PAIR_28_PENALTY=5.458163
PRIMER_LEFT_28_PENALTY=4.281296
PRIMER_RIGHT_28_PENALTY=1.176867
PRIMER_LEFT_28_SEQUENCE=GCATCCTACGATCCTTTATGTC
PRIMER_RIGHT_28_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_28=767,22
PRIMER_RIGHT_28=1463,21
PRIMER_LEFT_28_TM=57.719
PRIMER_RIGHT_28_TM=60.177
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.3000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=697
PRIMER_PAIR_29_PENALTY=5.480433
PRIMER_LEFT_29_PENALTY=3.750661
PRIMER_RIGHT_29_PENALTY=1.729772
PRIMER_LEFT_29_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_29_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_29=677,20
PRIMER_RIGHT_29=1463,20
PRIMER_LEFT_29_TM=56.249
PRIMER_RIGHT_29_TM=58.270
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=9.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=787
PRIMER_PAIR_30_PENALTY=5.486748
PRIMER_LEFT_30_PENALTY=3.756976
PRIMER_RIGHT_30_PENALTY=1.729772
PRIMER_LEFT_30_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_30_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_30=914,20
PRIMER_RIGHT_30=1463,20
PRIMER_LEFT_30_TM=56.243
PRIMER_RIGHT_30_TM=58.270
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.3000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=550
PRIMER_PAIR_31_PENALTY=5.512702
PRIMER_LEFT_31_PENALTY=4.335835
PRIMER_RIGHT_31_PENALTY=1.176867
PRIMER_LEFT_31_SEQUENCE=ACCCAGTTCAAAACGTAACA
PRIMER_RIGHT_31_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_31=676,20
PRIMER_RIGHT_31=1463,21
PRIMER_LEFT_31_TM=55.664
PRIMER_RIGHT_31_TM=60.177
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.0000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=788
PRIMER_PAIR_32_PENALTY=5.515183
PRIMER_LEFT_32_PENALTY=3.813243
PRIMER_RIGHT_32_PENALTY=1.701940
PRIMER_LEFT_32_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_32_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_32=886,20
PRIMER_RIGHT_32=1466,21
PRIMER_LEFT_32_TM=56.187
PRIMER_RIGHT_32_TM=59.298
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=581
PRIMER_PAIR_33_PENALTY=5.543015
PRIMER_LEFT_33_PENALTY=3.813243
PRIMER_RIGHT_33_PENALTY=1.729772
PRIMER_LEFT_33_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_33_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_33=886,20
PRIMER_RIGHT_33=1463,20
PRIMER_LEFT_33_TM=56.187
PRIMER_RIGHT_33_TM=58.270
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=578
PRIMER_PAIR_34_PENALTY=5.590453
PRIMER_LEFT_34_PENALTY=3.750661
PRIMER_RIGHT_34_PENALTY=1.839792
PRIMER_LEFT_34_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_34_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_34=677,20
PRIMER_RIGHT_34=1464,21
PRIMER_LEFT_34_TM=56.249
PRIMER_RIGHT_34_TM=59.160
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=788
PRIMER_PAIR_35_PENALTY=5.596768
PRIMER_LEFT_35_PENALTY=3.756976
PRIMER_RIGHT_35_PENALTY=1.839792
PRIMER_LEFT_35_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_35_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_35=914,20
PRIMER_RIGHT_35=1464,21
PRIMER_LEFT_35_TM=56.243
PRIMER_RIGHT_35_TM=59.160
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.3000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=7.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=551
PRIMER_PAIR_36_PENALTY=5.618147
PRIMER_LEFT_36_PENALTY=4.441280
PRIMER_RIGHT_36_PENALTY=1.176867
PRIMER_LEFT_36_SEQUENCE=CGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_36_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_36=755,21
PRIMER_RIGHT_36=1463,21
PRIMER_LEFT_36_TM=56.559
PRIMER_RIGHT_36_TM=60.177
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=709
PRIMER_PAIR_37_PENALTY=5.653035
PRIMER_LEFT_37_PENALTY=3.813243
PRIMER_RIGHT_37_PENALTY=1.839792
PRIMER_LEFT_37_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_37_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_37=886,20
PRIMER_RIGHT_37=1464,21
PRIMER_LEFT_37_TM=56.187
PRIMER_RIGHT_37_TM=59.160
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=579
PRIMER_PAIR_38_PENALTY=5.663303
PRIMER_LEFT_38_PENALTY=4.035956
PRIMER_RIGHT_38_PENALTY=1.627348
PRIMER_LEFT_38_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_38_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_38=752,24
PRIMER_RIGHT_38=1461,21
PRIMER_LEFT_38_TM=59.964
PRIMER_RIGHT_38_TM=59.373
PRIMER_LEFT_38_GC_PERCENT=41.667
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=710
PRIMER_PAIR_39_PENALTY=5.710410
PRIMER_LEFT_39_PENALTY=2.243575
PRIMER_RIGHT_39_PENALTY=3.466835
PRIMER_LEFT_39_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_39_SEQUENCE=TCAGTGTTTTAGGGATGATGG
PRIMER_LEFT_39=880,22
PRIMER_RIGHT_39=1467,21
PRIMER_LEFT_39_TM=60.244
PRIMER_RIGHT_39_TM=57.533
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=588
PRIMER_PAIR_40_PENALTY=5.717023
PRIMER_LEFT_40_PENALTY=4.540156
PRIMER_RIGHT_40_PENALTY=1.176867
PRIMER_LEFT_40_SEQUENCE=ACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_40_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_40=754,22
PRIMER_RIGHT_40=1463,21
PRIMER_LEFT_40_TM=57.460
PRIMER_RIGHT_40_TM=60.177
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=710
PRIMER_PAIR_41_PENALTY=5.737896
PRIMER_LEFT_41_PENALTY=4.035956
PRIMER_RIGHT_41_PENALTY=1.701940
PRIMER_LEFT_41_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_41_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_41=752,24
PRIMER_RIGHT_41=1466,21
PRIMER_LEFT_41_TM=59.964
PRIMER_RIGHT_41_TM=59.298
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=715
PRIMER_PAIR_42_PENALTY=5.765728
PRIMER_LEFT_42_PENALTY=4.035956
PRIMER_RIGHT_42_PENALTY=1.729772
PRIMER_LEFT_42_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_42_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_42=752,24
PRIMER_RIGHT_42=1463,20
PRIMER_LEFT_42_TM=59.964
PRIMER_RIGHT_42_TM=58.270
PRIMER_LEFT_42_GC_PERCENT=41.667
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=712
PRIMER_PAIR_43_PENALTY=5.799533
PRIMER_LEFT_43_PENALTY=2.243575
PRIMER_RIGHT_43_PENALTY=3.555959
PRIMER_LEFT_43_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_43_SEQUENCE=AGTGTTTTAGGGATGATGGG
PRIMER_LEFT_43=880,22
PRIMER_RIGHT_43=1465,20
PRIMER_LEFT_43_TM=60.244
PRIMER_RIGHT_43_TM=56.444
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=586
PRIMER_PAIR_44_PENALTY=5.817285
PRIMER_LEFT_44_PENALTY=3.750661
PRIMER_RIGHT_44_PENALTY=2.066624
PRIMER_LEFT_44_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_44_SEQUENCE=TTTAGGGATGATGGGTGAGA
PRIMER_LEFT_44=677,20
PRIMER_RIGHT_44=1460,20
PRIMER_LEFT_44_TM=56.249
PRIMER_RIGHT_44_TM=57.933
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=784
PRIMER_PAIR_45_PENALTY=5.854420
PRIMER_LEFT_45_PENALTY=4.227072
PRIMER_RIGHT_45_PENALTY=1.627348
PRIMER_LEFT_45_SEQUENCE=AACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_45_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_45=753,23
PRIMER_RIGHT_45=1461,21
PRIMER_LEFT_45_TM=58.773
PRIMER_RIGHT_45_TM=59.373
PRIMER_LEFT_45_GC_PERCENT=43.478
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=709
PRIMER_PAIR_46_PENALTY=5.854880
PRIMER_LEFT_46_PENALTY=2.243575
PRIMER_RIGHT_46_PENALTY=3.611306
PRIMER_LEFT_46_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_46_SEQUENCE=AGTGTTTTAGGGATGATGGGT
PRIMER_LEFT_46=880,22
PRIMER_RIGHT_46=1465,21
PRIMER_LEFT_46_TM=60.244
PRIMER_RIGHT_46_TM=57.389
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.6000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=586
PRIMER_PAIR_47_PENALTY=5.860171
PRIMER_LEFT_47_PENALTY=3.750661
PRIMER_RIGHT_47_PENALTY=2.109510
PRIMER_LEFT_47_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_47_SEQUENCE=AGTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_47=677,20
PRIMER_RIGHT_47=1465,22
PRIMER_LEFT_47_TM=56.249
PRIMER_RIGHT_47_TM=60.110
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=789
PRIMER_PAIR_48_PENALTY=5.860171
PRIMER_LEFT_48_PENALTY=3.750661
PRIMER_RIGHT_48_PENALTY=2.109510
PRIMER_LEFT_48_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_48_SEQUENCE=CAGTGTTTTAGGGATGATGGGT
PRIMER_LEFT_48=677,20
PRIMER_RIGHT_48=1466,22
PRIMER_LEFT_48_TM=56.249
PRIMER_RIGHT_48_TM=60.110
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=790
PRIMER_PAIR_49_PENALTY=5.866486
PRIMER_LEFT_49_PENALTY=3.756976
PRIMER_RIGHT_49_PENALTY=2.109510
PRIMER_LEFT_49_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_49_SEQUENCE=AGTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_49=914,20
PRIMER_RIGHT_49=1465,22
PRIMER_LEFT_49_TM=56.243
PRIMER_RIGHT_49_TM=60.110
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.3000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=552
=
SEQUENCE_ID=MAGIC.338_2_6550882_6550902_po-0_ler-0_156544_6536_TRUE
SEQUENCE_TARGET=950,120
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_12846393_12846608_can-0_ler-0_161417_521_TRUE
SEQUENCE_TARGET=950,315
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNGTGNCTATAGNCAATAAAAAANCATTTGTTTCATGTTCCNAAAAATANAAANANANTTTTAGGNCCCCCNAATCTTGTAAATATAGTGTATNAGAGTGGTTTTAGANGACTCTTTTATTNCGTGAGCACCCAAGTTCGANTCTTTTGAACAANNNNNTTTTACNTATATACACAATTAGGNTTTANAAANAGAAAATTGAGAATGGATTNAGAGAGATAGTTTATTGAGACANAAGGANAGANAATTGTATCTTATTAGTTANTTNTTACTNTTTTGTNTTNAANANTTTATATGNTTTNAAAAANAAAATNGATTTATTTTATTTGAAAAGNTCTATNTATTTANTTTTTCTTCTTGATCCCAANTTTANCTTTGCCTTAANCNTNGGCACGTNTTGCAGCGNGATAANTTATGAAGAACCGAGACANTGCTTTTAAGGANAGAATACGAAANTAATNTTGAGTNTNAATNGATCTCAAGNANGACANTCGTAGCTCNNAATTNTANTNNNATNNNNCTTCAAAGACTTCGTTTGANAATACTNTTCTNAGTGCNTATCAAAANCTCTTNAATATTATTAATCAAATCAACAACNNAATAATACANTNGGCCTAAGANGGCTATATATAATAAAAGCTCAAAACCCTAAANATAGAATATTCTAGAATAANTAANTAAAAGATAANAATANTAGATNTTTGNNNATNTTCCAAATATCTNGCTGCATCANCACGACACNGGNAGTAGAGCACAACANTTNGACAATANGCTATNATTATAAACNANNCNAACAACACNTTGAATAATAGAGAACACAAACTACAGTTAGATTNCAAAGTACNNNNNNNNNNNNNNNNNNNNNNNNGTTAATTTTTACGTTTATTANAAAGTGGGTTAAGTTGAAGTTTGGCCACAAAACAAATNAAAAGNCCAACACANTAGACTNCATCTTCTCCAATCTACACAAGNACGATTCNGATCAACATCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_129599_129703_sf-2_can-0_62538_203134_TRUE
SEQUENCE_TARGET=950,204
SEQUENCE_TEMPLATE=AAGGNTGTGCATGGTTATAATAATAGTTATTAGAAACAAATAGAAGNACGANATAAAAAGACAAGGAGATCGATAACAGGTCATGAGTNNNNTATATGTGGTCTTACATATATACAAAATTGTGAGGAGGCTGATGTAGCTCCCAATGTNGTATATGATGGNCACATAAAGGATGGTCTGCCACATGAANNTTTATATAAATGAATCNTTAAANTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTAGTGAAGATTCACACCTTTAGAAANGTTTTGTTGTCTTTCCCATATGCAATCTCATAGAGCATGAGGAGATGCCCCTCCATCATTNAAAACAAGGNTTTAAGTGGNGAATCTGGTTGNTTTAACTCTTCTGGAGTTGGAGGTCTCCTGTGTAANATCACATAATTGGTGAAACGTTAAGTAAGAGNTNGCTCTATNTACTGTTATATTGTAAAGACGNNNGTATTTGGTAGGTACCGAGTGAAAAAGACTTCTCCGAACTTGNCCCAAAGGAAGAGAANGAGCATGAGGAGTAACAAGATTGAGCAGAGAAGCGGAACTAATCGAGCTTCCATGAACTCCAAGAGNAACCAGAAGAGTGTGGCCATTACAAGTGTTATCCCTGAGTTTATCTTGTCCTTCCACAGACATAGATCTGCCACTNCAACAACATTNCCAATTGGTTATTAATCGTAANGTNAAGCCGGTTAGTCTCTTCTTTGANTAGATTTTGTTTTCCTCTACAAGAATGTCAGGTCTTAATCATATTCTACTAAGTGAAAGNATTGTGCATGGATTCATATTNAAANANNATNAGTATTTGATAATGATGTAACCTGCTCCNTCGCCAAGTATTTGATCCACAAGAGACATNNTGGTTGTGTTGTGATAATTCCTTANTTCTTAAAGGAGACCTGCAATATAATCTCCNTAGANCATACTCAATCGGGATTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTAACACTTTATTACTTNATAANACACGAACTANTGACTTCTCAACCCGCNTGAAANTCTTGGCTCGCAAGACANTTTATTTAGTGCAATNANNNTTNNNTNNNNANAGTATTGTGTTGCATTCTATTCTTATTATGGAANNGCATTGCAGAGTTGCATGGGAAGCGTTTNANGANTNCNAATNTTTGTNNNTTGNGACAGAGAAGCNTTGTTAGGTATTTACGACTGTCATTATNCTNCGAGCTGATTAGAATCTGTAATCGNANT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4741819_4741920_can-0_bur-0_2734_49895_TRUE
SEQUENCE_TARGET=950,201
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNATTCTACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_4548657_4548668_can-0_oy-0_26887_3341_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4507001_4507080_rsch-4_ler-0_25702_84476_TRUE
SEQUENCE_TARGET=950,179
SEQUENCE_TEMPLATE=ANGGNNTTTTGCNNTTAANNTGTGGNTTTGCAAANTCCANNTTTTGGAAATAGNTTNNGTTAACCATGTGGNCAAGTTATGANATTCANAGGCTGATNAGACCTATCTCTTNAAANGTGAGTCTGAANTNGNTTTAANTNCGNGAATTCCCCAACTCGGGTTCNNTCAATTCGNCTTATNTNCTGGGCTTGGGNCTTANNNNNTTTTNTTNTATNTAATNAANNNTTTGTCTTTAATGCGACGGTTGCCATTTNNTTNCTNNGNAAATAGNAAAAGCNTCACNCCTNTNAGGCANTANTTNNNNNNNCCTCTATTTCCAAAAATGGTTTAAACCATTNGGACNCGCCANAATTATTANTTTTGTGGTTTNANNAANANCCCTAACTCNCTANANNTAAGAGCACAAACTNTNNNNNATGTNTCAAAANATGATCTCAGAAATTTACTTGGTAAANACTTCAAGNANGGAAAACGAANGANCCTCCACCNTNTGTCCTCTTGACCCNGNNAGCCCACTGNTTCTGTNCGGCGATGGCTTNGAGCCCGCCNNCGTNCTCTTTCCTCACNCNGNNTTTCACTTCCCNNCGAAGGANAANANNNNNNNNTACNATTTNATGGNAAANGTAGGTCTNTTACNCTTTTNCTTANCNNANNNNNTNCNNNNNNNNNNNNNNNNNNNNNNNNCTAGGTNNTCGACAAGCANCNCCGCCTATGCGATGNNNNNNNNNNNNNNNNNNNNNAGAGGNAAANGCTCNAGGCCCGAATTNCCCGNATNGAAAGGAAGGTGAGGGCCATNNAGAGNGATCCCTTCNAATNNAGGAACTTCGACTGTGTGGCGAAGATCCNGCACATGCTNCGGATGTANCTCNGTGCTCGAGGTNNAGTNNNNNNNNNNNNNNNNNNNNTGGTCCAAGTGATNCCGNCTGACNGNNAAGAGNNNGANGACGAGGANGANGACANCNACCCTTGGAAGNGNGTGAAGAATGAACCCAAAGTNGACNTCAANAACNAGGCATCNACCTCNAACCCNGAACCGGCGATGAANGAGCNTGTTCCCGAGCNNCTTCCCTAAATGTCCCNCNANTTCNNATNNNTNNNNNTTTTTTNNATTTTTGTTTGGACTTNNTNTTGGGNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNAAATATTCGTTTCCGAGATACTCTTTGAATGCAGGGNGATNNGGNNGACGTGAATGCTTCCGCNAATCACNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAAGTTACGAGTCNTTATAAATTGNATCNTNTATGTTTTCTTTATTCNTCATCTAACTTTNGTTTTTCCNTTTNTGNAGTTATGGCTTTTAACCNTCGNNNNNNNNNNNNNATGCCANACTTTTNCANNNNNNNGATGATNGATGANGATATNCNGAANGTGCCGGACAGAGNACGCTNNCCACTTNGNAACTTNAGATCCCAAACCCGAGCTCCGTTTGCANTAATCAAGGATNTTTCNANCAGAGANCNANAAATTGCNNTGGCCTTNATCAAAGCNCTCGNGGCTNAACACNANAAGNGTTCNTCAGAAAANAAGANACTTNGAAAGAANATCAANNANANGNNNNNNNNNNNNNNNATGATATCAAGGGAGNTACACACCCNCANNTCNCATCCNGGGGACTGGATNGANCNNNAANNNNNNNNNNNNNNNNNNNNNNNNNATTGTNNNNNNNNNNNNNNNNATCTTGCCAACCAAGGAGTCNAAGTNTTTTNCAAGACGAGAAGGATTTNCTAATCCGAAGTGTGTTTAAGACTTCNNCATCNTTTTATNGTTTTCGCCNTTGAANNNNNNTANCTTTGCTTCCTGNTTTGTATGTTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=5.191381
PRIMER_LEFT_0_PENALTY=1.706282
PRIMER_RIGHT_0_PENALTY=3.485099
PRIMER_LEFT_0_SEQUENCE=TTGTCTTTAATGCGACGGTT
PRIMER_RIGHT_0_SEQUENCE=CAAAGAGTATCTCGGAAACGAA
PRIMER_LEFT_0=226,20
PRIMER_RIGHT_0=1209,22
PRIMER_LEFT_0_TM=58.294
PRIMER_RIGHT_0_TM=58.515
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=984
=
SEQUENCE_ID=MAGIC.175_3_13163998_13164022_ws-0_can-0_2719_6896_TRUE
SEQUENCE_TARGET=950,124
SEQUENCE_TEMPLATE=GGCCNAAGCCCANTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACATGGTTAANNNNNNNNNNNNNNNANANGATGNACTTTGCAAATCCANANTTAAATATAGCNAAAANCNTCTTNGGATCNTNNTCTNAAAGGTTTNNNNNNNNNNNNNNNNNAACACTTGTTCAGAAANNAAAAAGNCTNCTTAGGCGATNANANTANACTNANAAATAAACTTCAAGTTNNTAAAGNGACAACCCCCATTAAGTGGACGCANCCACNANNGATCCTNAGCCACCTCNAANTCAGTNNGAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATNNNNNNNNNNNNNNNNNNNCNGTTCNNTNGGNATCCNAGNNTNCNAACCATNNTNANCCTNGATGACCACTGTTNCGNNNTTCNTGGATGGANGAATTTNNGNNGGNNNGGNNNNNNNNNNNNNNNNNNNNNNNNNCATGATAGNTACNTCNNGTTCCANGGGATCACTNGGACNTNNGATAATNATCTCGANTCAGGNAAACGNAAGTNTAANTTANTTNGGNTTGGTGTCANAGTTTGANGTTTTNAAANNTACNAGNCNNNNNNNNNNNNNNNNNNNNCAAAGNAGGCGACTCGGTAAAGAACTNNTNNAANTGTTNANGGTTGAATGANNAAGNGGGNAGAGCGGCCNANNGACNTNGCTTCTNTNNTAAAACGGNTTNCAATTCGGACCNNNNNTGNTNCTCNGAAAAATCCTCNTCNTGGGTTTGGTCNAGAAGGAGGAGGNTNCTTTCATANTCCTTGATNTCTGATTNNAGNTCNGTCNNAGTTTTCTCGNTTTGGATGAAAANGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACNTCTTTCCTGGCTTTNNNNNNNNNNNNNNCTAACTNAGCATCTCNNAGTTTGGNNATTGCTTTGTANNTGGCCTGCANTTTGGATAGCTGGTTGNACAAGTCGTAAGCANTNGATNNAGATTCNGAGAGTCTGCCNCGAGNATTCNAGAGTTCCTCCTGNATCTTGTCATACCNNTGATACTNATCCTGCAACTCGNNGTTNGACNNCATGAGTNTGNANNNNNNNNNNNNNANTTGATCTTCNNATNTTNNCTTGACTTCNGTGNAAGAGNGGAGATCGGACTNAAGAGAANAANACTTATCNTTCNAGGCCATAACNTCNTGTTCCCTNTCAAGCAACAACCTNTCGTAATACTNAATCATGTCATNGAGTTCGCTGTTTGTCTGAACCAANGNTTACAAAGGTGAGTTNGNGTATAANNNAAAGAGTNGAGCTAAANNGTCACAAATAAAGANANCTAACCCGAGATGAGTGATGGGCNNTGATATGACTATTGTCNGACAAGACTTGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_7399517_7399754_ler-0_ws-0_653204_715319_TRUE
SEQUENCE_TARGET=950,337
SEQUENCE_TEMPLATE=TNAAAAATATTGTTTCTGTAAAGATTAAACAGTATAATATCACTACANTTTAGCTTTTTGGCATATTTGCCTCTTTCTTTTCAANTGAAATATAGNTTTATATATTTAAAAGCTTTNAAACATGACNGCCGTTGTTACGTTTACAAGAGTAAAAAGTGGATAAGAATCCTGTATNTTTTTTTTNTTNTTACAATAAATAACTTCTATCAATAGTGATGAAATTAAGTCGAATCTATATATANTTTGAANTTTGACTAAATNCATTGACNNNNNNNNNTTTNNNNNNNNNATAAGAAATCCATTGACTTGTAAAAATCTTATNTCCTANGAAAATTTCCAAGATAAAGTTTCTCTCTAGCTTNTACNTTTGCAATTACAGTATAAGTTAGAGAGGTNGTCATATTTTCTGCTAGGGATACAATANGTAATGAAGTCANGAAGANAAAGCAGCCGACCATTGGGNAATGAATATGCACTTGTGACTTTGTNAGTTGTTGGTGAATAANTTCGTCTGAGTGAATAAAACTAGTTCTAAGAGANAATATGACATATTNAAACACTTTTATTACTCGNAGNNAAAGATATCAAGAAAGAATGNCAACAAAGATGNAAATANATAATTAACGACGATTTTGTTTTACATTTCACACATTTTATTTACAGAACTCAATAATCCAGCAGTGATAAGCTTGGCAAACGNANTTGCAATGGGTAAAGCCAGAAGCTGCAGCCAAAGCTTCAAACTCAGCTCTTGATCTCTCTTTTCCTCCGGAACATTGNGTGAACATTAACATGTCCATGTCAAAGGCAATGTTTGCGTTGATGTCTCCATTNTCTGCCTCNTCAGGAGTNACTAATTCTATNACAACNACTTTACCGNTTTCTGGTAGTGATNTCCAACAATTCTTAAGAATCTTGACACAATCTTCATCNGTCCAATCATGAAGTATACGCTATAAAAACATNATANNATTAGTATNAAGTNCTANTANCATGTATANACTTCCAAAACATATAAGTAATAAGTNNTNTTGAGCTTACTTTCAAGATCATGGCATCTCCGGTTGGNANATCCACAAACATATCTCCNGCAACATGNTCCACACCGGGATAAGANGGTGCTTGTGCCAAGGCACAGGTTAGATCAAANTTGATACCCTTAATATTTGGATACTTAGAAGNAACAACACCAAGAGTGTTACCAACTCCTCCTCCCACATCAACTAANACATTCACANCTTTGAANCCTTGGTAAACTTCAAGAGCCTTCTTCACNACCGCAATGGTAAAACCNGTCTGGTTAAATAGTTTGCTGAATCTCTCATCTGTACCCATATAGTCAAAGAGNTTCATACCACCATGTGCGCGGCCAAATGCATCTCCTCCTTCTAGAACCACGTCTTTCAATTGTGCCCTGAAATTTATGTATCGATNNAGGTAAGATTCATTGAATATAAANCCACTTCNTGATNAATTATAAGACATCCATGANTAATGAAAATTACCAAGTATTGAGGAAGACGCTATCGAAATTGACAATGACTTGAGAAGCAAGGGATCCAATATCTTGAATGTTATCCTTCAAGAAAAACCTGCAAATTGGCTCNGCTCTGTAGACCCTCTCACCCTTTCCTNCNGAGACCTTACCACACTTGACCACAGAATAGCTAGCGAGTAGACGAAGCATACGGTCCAANAAAACCGGAGCCTCAGGGTTACGAGGTGTAGTTGGTAGCTTACTCGCTATTTCNGANGGTGAAAGGAACGAGTCGCTGCGAGAGGCTTCAGCATAGAGAGTGTCAAAGACACCNAGCTCGAGGGCGGCTTTGAGAACCATCGGAAAGGCAGNAGCATTGGCTAGTCTCACGGCCATCAAACCNAACTCGTTATCATCATCGATAACAATTGGGGTTTTAGAGTTAGAGCTTAANGTTTCTTCTANAAGGTATCCCATTTTTATTTCTTTTTGTTTCNTTCTTCTCCTTCAAGGCACTCTACGTGATCACACNGATCAGAAGTGAGGAAANATGTATNGAGANAGAGAGANAGNNAGTGTGTTTCTTCTTTGTAGTGNTGATGATTTAGTATGTTTTTGATGTATATCCTTGTTTCTCCTTTTATAGCTAAAGCTTAGATATTTCTNCCTTTTNTTNATAAAATTTTCACATAAATCATTGTGGCTAGCGTTGCNGGTCCAGCTTGGTTGCTGAATAGTCTCTATNTNATGTACCGTAGAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.663451
PRIMER_LEFT_0_PENALTY=0.292414
PRIMER_RIGHT_0_PENALTY=0.371037
PRIMER_LEFT_0_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_0_SEQUENCE=ATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_0=704,20
PRIMER_RIGHT_0=1563,20
PRIMER_LEFT_0_TM=59.708
PRIMER_RIGHT_0_TM=59.629
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=860
PRIMER_PAIR_1_PENALTY=1.005641
PRIMER_LEFT_1_PENALTY=0.292414
PRIMER_RIGHT_1_PENALTY=0.713227
PRIMER_LEFT_1_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_1_SEQUENCE=TTGGATCCCTTGCTTCTCAA
PRIMER_LEFT_1=704,20
PRIMER_RIGHT_1=1562,20
PRIMER_LEFT_1_TM=59.708
PRIMER_RIGHT_1_TM=60.713
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=859
PRIMER_PAIR_2_PENALTY=1.045466
PRIMER_LEFT_2_PENALTY=0.292414
PRIMER_RIGHT_2_PENALTY=0.753052
PRIMER_LEFT_2_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_2_SEQUENCE=TTCAGGGCACAATTGAAAGA
PRIMER_LEFT_2=704,20
PRIMER_RIGHT_2=1418,20
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=59.247
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=715
PRIMER_PAIR_3_PENALTY=1.087066
PRIMER_LEFT_3_PENALTY=0.292414
PRIMER_RIGHT_3_PENALTY=0.794652
PRIMER_LEFT_3_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_3_SEQUENCE=GCCAATTTGCAGGTTTTTCT
PRIMER_LEFT_3=704,20
PRIMER_RIGHT_3=1603,20
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=59.205
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=900
PRIMER_PAIR_4_PENALTY=1.087066
PRIMER_LEFT_4_PENALTY=0.292414
PRIMER_RIGHT_4_PENALTY=0.794652
PRIMER_LEFT_4_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_4_SEQUENCE=AGCCAATTTGCAGGTTTTTC
PRIMER_LEFT_4=704,20
PRIMER_RIGHT_4=1604,20
PRIMER_LEFT_4_TM=59.708
PRIMER_RIGHT_4_TM=59.205
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=901
PRIMER_PAIR_5_PENALTY=1.087066
PRIMER_LEFT_5_PENALTY=0.292414
PRIMER_RIGHT_5_PENALTY=0.794652
PRIMER_LEFT_5_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_5_SEQUENCE=GAGCCAATTTGCAGGTTTTT
PRIMER_LEFT_5=704,20
PRIMER_RIGHT_5=1605,20
PRIMER_LEFT_5_TM=59.708
PRIMER_RIGHT_5_TM=59.205
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=902
PRIMER_PAIR_6_PENALTY=1.173812
PRIMER_LEFT_6_PENALTY=0.516718
PRIMER_RIGHT_6_PENALTY=0.657094
PRIMER_LEFT_6_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_6_SEQUENCE=TTGGACCGTATGCTTCGTCT
PRIMER_LEFT_6=791,20
PRIMER_RIGHT_6=1695,20
PRIMER_LEFT_6_TM=60.517
PRIMER_RIGHT_6_TM=60.657
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.0000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=905
PRIMER_PAIR_7_PENALTY=1.176135
PRIMER_LEFT_7_PENALTY=0.805098
PRIMER_RIGHT_7_PENALTY=0.371037
PRIMER_LEFT_7_SEQUENCE=CAGCCAAAGCTTCAAACTCA
PRIMER_RIGHT_7_SEQUENCE=ATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_7=727,20
PRIMER_RIGHT_7=1563,20
PRIMER_LEFT_7_TM=59.195
PRIMER_RIGHT_7_TM=59.629
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=837
PRIMER_PAIR_8_PENALTY=1.229945
PRIMER_LEFT_8_PENALTY=0.516718
PRIMER_RIGHT_8_PENALTY=0.713227
PRIMER_LEFT_8_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_8_SEQUENCE=TTGGATCCCTTGCTTCTCAA
PRIMER_LEFT_8=791,20
PRIMER_RIGHT_8=1562,20
PRIMER_LEFT_8_TM=60.517
PRIMER_RIGHT_8_TM=60.713
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=10.0000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=772
PRIMER_PAIR_9_PENALTY=1.244307
PRIMER_LEFT_9_PENALTY=0.873270
PRIMER_RIGHT_9_PENALTY=0.371037
PRIMER_LEFT_9_SEQUENCE=TTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_9_SEQUENCE=ATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_9=813,20
PRIMER_RIGHT_9=1563,20
PRIMER_LEFT_9_TM=59.127
PRIMER_RIGHT_9_TM=59.629
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=751
PRIMER_PAIR_10_PENALTY=1.269770
PRIMER_LEFT_10_PENALTY=0.516718
PRIMER_RIGHT_10_PENALTY=0.753052
PRIMER_LEFT_10_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_10_SEQUENCE=TTCAGGGCACAATTGAAAGA
PRIMER_LEFT_10=791,20
PRIMER_RIGHT_10=1418,20
PRIMER_LEFT_10_TM=60.517
PRIMER_RIGHT_10_TM=59.247
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=10.0000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=628
PRIMER_PAIR_11_PENALTY=1.311370
PRIMER_LEFT_11_PENALTY=0.516718
PRIMER_RIGHT_11_PENALTY=0.794652
PRIMER_LEFT_11_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_11_SEQUENCE=GCCAATTTGCAGGTTTTTCT
PRIMER_LEFT_11=791,20
PRIMER_RIGHT_11=1603,20
PRIMER_LEFT_11_TM=60.517
PRIMER_RIGHT_11_TM=59.205
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.0000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=813
PRIMER_PAIR_12_PENALTY=1.311370
PRIMER_LEFT_12_PENALTY=0.516718
PRIMER_RIGHT_12_PENALTY=0.794652
PRIMER_LEFT_12_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_12_SEQUENCE=AGCCAATTTGCAGGTTTTTC
PRIMER_LEFT_12=791,20
PRIMER_RIGHT_12=1604,20
PRIMER_LEFT_12_TM=60.517
PRIMER_RIGHT_12_TM=59.205
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.0000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=814
PRIMER_PAIR_13_PENALTY=1.311370
PRIMER_LEFT_13_PENALTY=0.516718
PRIMER_RIGHT_13_PENALTY=0.794652
PRIMER_LEFT_13_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_13_SEQUENCE=GAGCCAATTTGCAGGTTTTT
PRIMER_LEFT_13=791,20
PRIMER_RIGHT_13=1605,20
PRIMER_LEFT_13_TM=60.517
PRIMER_RIGHT_13_TM=59.205
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=815
PRIMER_PAIR_14_PENALTY=1.325028
PRIMER_LEFT_14_PENALTY=0.292414
PRIMER_RIGHT_14_PENALTY=1.032614
PRIMER_LEFT_14_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_14_SEQUENCE=TTTCGATAGCGTCTTCCTCAA
PRIMER_LEFT_14=704,20
PRIMER_RIGHT_14=1531,21
PRIMER_LEFT_14_TM=59.708
PRIMER_RIGHT_14_TM=59.967
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=828
PRIMER_PAIR_15_PENALTY=1.378682
PRIMER_LEFT_15_PENALTY=1.007644
PRIMER_RIGHT_15_PENALTY=0.371037
PRIMER_LEFT_15_SEQUENCE=GTTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_15_SEQUENCE=ATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_15=812,21
PRIMER_RIGHT_15=1563,20
PRIMER_LEFT_15_TM=59.992
PRIMER_RIGHT_15_TM=59.629
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=752
PRIMER_PAIR_16_PENALTY=1.378682
PRIMER_LEFT_16_PENALTY=1.007644
PRIMER_RIGHT_16_PENALTY=0.371037
PRIMER_LEFT_16_SEQUENCE=AATGTTTGCGTTGATGTCTCC
PRIMER_RIGHT_16_SEQUENCE=ATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_16=809,21
PRIMER_RIGHT_16=1563,20
PRIMER_LEFT_16_TM=59.992
PRIMER_RIGHT_16_TM=59.629
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=755
PRIMER_PAIR_17_PENALTY=1.395519
PRIMER_LEFT_17_PENALTY=0.292414
PRIMER_RIGHT_17_PENALTY=1.103105
PRIMER_LEFT_17_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_17_SEQUENCE=TTCAGGGCACAATTGAAAGAC
PRIMER_LEFT_17=704,20
PRIMER_RIGHT_17=1418,21
PRIMER_LEFT_17_TM=59.708
PRIMER_RIGHT_17_TM=60.103
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=715
PRIMER_PAIR_18_PENALTY=1.462191
PRIMER_LEFT_18_PENALTY=0.805098
PRIMER_RIGHT_18_PENALTY=0.657094
PRIMER_LEFT_18_SEQUENCE=CAGCCAAAGCTTCAAACTCA
PRIMER_RIGHT_18_SEQUENCE=TTGGACCGTATGCTTCGTCT
PRIMER_LEFT_18=727,20
PRIMER_RIGHT_18=1695,20
PRIMER_LEFT_18_TM=59.195
PRIMER_RIGHT_18_TM=60.657
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=969
PRIMER_PAIR_19_PENALTY=1.497320
PRIMER_LEFT_19_PENALTY=0.292414
PRIMER_RIGHT_19_PENALTY=1.204906
PRIMER_LEFT_19_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_19_SEQUENCE=GGATCCCTTGCTTCTCAAGTC
PRIMER_LEFT_19=704,20
PRIMER_RIGHT_19=1560,21
PRIMER_LEFT_19_TM=59.708
PRIMER_RIGHT_19_TM=60.205
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=857
PRIMER_PAIR_20_PENALTY=1.518325
PRIMER_LEFT_20_PENALTY=0.805098
PRIMER_RIGHT_20_PENALTY=0.713227
PRIMER_LEFT_20_SEQUENCE=CAGCCAAAGCTTCAAACTCA
PRIMER_RIGHT_20_SEQUENCE=TTGGATCCCTTGCTTCTCAA
PRIMER_LEFT_20=727,20
PRIMER_RIGHT_20=1562,20
PRIMER_LEFT_20_TM=59.195
PRIMER_RIGHT_20_TM=60.713
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=836
PRIMER_PAIR_21_PENALTY=1.530364
PRIMER_LEFT_21_PENALTY=0.873270
PRIMER_RIGHT_21_PENALTY=0.657094
PRIMER_LEFT_21_SEQUENCE=TTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_21_SEQUENCE=TTGGACCGTATGCTTCGTCT
PRIMER_LEFT_21=813,20
PRIMER_RIGHT_21=1695,20
PRIMER_LEFT_21_TM=59.127
PRIMER_RIGHT_21_TM=60.657
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=883
PRIMER_PAIR_22_PENALTY=1.547336
PRIMER_LEFT_22_PENALTY=0.292414
PRIMER_RIGHT_22_PENALTY=1.254922
PRIMER_LEFT_22_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_22_SEQUENCE=TTTCAGGGCACAATTGAAAG
PRIMER_LEFT_22=704,20
PRIMER_RIGHT_22=1419,20
PRIMER_LEFT_22_TM=59.708
PRIMER_RIGHT_22_TM=58.745
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=716
PRIMER_PAIR_23_PENALTY=1.549332
PRIMER_LEFT_23_PENALTY=0.516718
PRIMER_RIGHT_23_PENALTY=1.032614
PRIMER_LEFT_23_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_23_SEQUENCE=TTTCGATAGCGTCTTCCTCAA
PRIMER_LEFT_23=791,20
PRIMER_RIGHT_23=1531,21
PRIMER_LEFT_23_TM=60.517
PRIMER_RIGHT_23_TM=59.967
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=10.0000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=741
PRIMER_PAIR_24_PENALTY=1.558150
PRIMER_LEFT_24_PENALTY=0.805098
PRIMER_RIGHT_24_PENALTY=0.753052
PRIMER_LEFT_24_SEQUENCE=CAGCCAAAGCTTCAAACTCA
PRIMER_RIGHT_24_SEQUENCE=TTCAGGGCACAATTGAAAGA
PRIMER_LEFT_24=727,20
PRIMER_RIGHT_24=1418,20
PRIMER_LEFT_24_TM=59.195
PRIMER_RIGHT_24_TM=59.247
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=692
PRIMER_PAIR_25_PENALTY=1.586497
PRIMER_LEFT_25_PENALTY=0.873270
PRIMER_RIGHT_25_PENALTY=0.713227
PRIMER_LEFT_25_SEQUENCE=TTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_25_SEQUENCE=TTGGATCCCTTGCTTCTCAA
PRIMER_LEFT_25=813,20
PRIMER_RIGHT_25=1562,20
PRIMER_LEFT_25_TM=59.127
PRIMER_RIGHT_25_TM=60.713
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=750
PRIMER_PAIR_26_PENALTY=1.595364
PRIMER_LEFT_26_PENALTY=0.292414
PRIMER_RIGHT_26_PENALTY=1.302950
PRIMER_LEFT_26_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_26_SEQUENCE=TCAGGGCACAATTGAAAGAC
PRIMER_LEFT_26=704,20
PRIMER_RIGHT_26=1417,20
PRIMER_LEFT_26_TM=59.708
PRIMER_RIGHT_26_TM=58.697
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=714
PRIMER_PAIR_27_PENALTY=1.599750
PRIMER_LEFT_27_PENALTY=0.805098
PRIMER_RIGHT_27_PENALTY=0.794652
PRIMER_LEFT_27_SEQUENCE=CAGCCAAAGCTTCAAACTCA
PRIMER_RIGHT_27_SEQUENCE=GAGCCAATTTGCAGGTTTTT
PRIMER_LEFT_27=727,20
PRIMER_RIGHT_27=1605,20
PRIMER_LEFT_27_TM=59.195
PRIMER_RIGHT_27_TM=59.205
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=879
PRIMER_PAIR_28_PENALTY=1.619823
PRIMER_LEFT_28_PENALTY=0.516718
PRIMER_RIGHT_28_PENALTY=1.103105
PRIMER_LEFT_28_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_28_SEQUENCE=TTCAGGGCACAATTGAAAGAC
PRIMER_LEFT_28=791,20
PRIMER_RIGHT_28=1418,21
PRIMER_LEFT_28_TM=60.517
PRIMER_RIGHT_28_TM=60.103
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=10.0000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=628
PRIMER_PAIR_29_PENALTY=1.638647
PRIMER_LEFT_29_PENALTY=0.292414
PRIMER_RIGHT_29_PENALTY=1.346233
PRIMER_LEFT_29_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_29_SEQUENCE=TATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_29=704,20
PRIMER_RIGHT_29=1564,21
PRIMER_LEFT_29_TM=59.708
PRIMER_RIGHT_29_TM=59.654
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=861
PRIMER_PAIR_30_PENALTY=1.647770
PRIMER_LEFT_30_PENALTY=1.276733
PRIMER_RIGHT_30_PENALTY=0.371037
PRIMER_LEFT_30_SEQUENCE=TGTTTGCGTTGATGTCTCCA
PRIMER_RIGHT_30_SEQUENCE=ATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_30=811,20
PRIMER_RIGHT_30=1563,20
PRIMER_LEFT_30_TM=61.277
PRIMER_RIGHT_30_TM=59.629
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=753
PRIMER_PAIR_31_PENALTY=1.664738
PRIMER_LEFT_31_PENALTY=1.007644
PRIMER_RIGHT_31_PENALTY=0.657094
PRIMER_LEFT_31_SEQUENCE=GTTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_31_SEQUENCE=TTGGACCGTATGCTTCGTCT
PRIMER_LEFT_31=812,21
PRIMER_RIGHT_31=1695,20
PRIMER_LEFT_31_TM=59.992
PRIMER_RIGHT_31_TM=60.657
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=884
PRIMER_PAIR_32_PENALTY=1.664738
PRIMER_LEFT_32_PENALTY=1.007644
PRIMER_RIGHT_32_PENALTY=0.657094
PRIMER_LEFT_32_SEQUENCE=AATGTTTGCGTTGATGTCTCC
PRIMER_RIGHT_32_SEQUENCE=TTGGACCGTATGCTTCGTCT
PRIMER_LEFT_32=809,21
PRIMER_RIGHT_32=1695,20
PRIMER_LEFT_32_TM=59.992
PRIMER_RIGHT_32_TM=60.657
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=887
PRIMER_PAIR_33_PENALTY=1.667922
PRIMER_LEFT_33_PENALTY=0.873270
PRIMER_RIGHT_33_PENALTY=0.794652
PRIMER_LEFT_33_SEQUENCE=TTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_33_SEQUENCE=GCCAATTTGCAGGTTTTTCT
PRIMER_LEFT_33=813,20
PRIMER_RIGHT_33=1603,20
PRIMER_LEFT_33_TM=59.127
PRIMER_RIGHT_33_TM=59.205
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=791
PRIMER_PAIR_34_PENALTY=1.667922
PRIMER_LEFT_34_PENALTY=0.873270
PRIMER_RIGHT_34_PENALTY=0.794652
PRIMER_LEFT_34_SEQUENCE=TTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_34_SEQUENCE=AGCCAATTTGCAGGTTTTTC
PRIMER_LEFT_34=813,20
PRIMER_RIGHT_34=1604,20
PRIMER_LEFT_34_TM=59.127
PRIMER_RIGHT_34_TM=59.205
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.4000
PRIMER_RIGHT_34_END_STABILITY=7.3000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=792
PRIMER_PAIR_35_PENALTY=1.667922
PRIMER_LEFT_35_PENALTY=0.873270
PRIMER_RIGHT_35_PENALTY=0.794652
PRIMER_LEFT_35_SEQUENCE=TTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_35_SEQUENCE=GAGCCAATTTGCAGGTTTTT
PRIMER_LEFT_35=813,20
PRIMER_RIGHT_35=1605,20
PRIMER_LEFT_35_TM=59.127
PRIMER_RIGHT_35_TM=59.205
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.4000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=793
PRIMER_PAIR_36_PENALTY=1.693753
PRIMER_LEFT_36_PENALTY=0.292414
PRIMER_RIGHT_36_PENALTY=1.401339
PRIMER_LEFT_36_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_36_SEQUENCE=TTCGATAGCGTCTTCCTCAA
PRIMER_LEFT_36=704,20
PRIMER_RIGHT_36=1530,20
PRIMER_LEFT_36_TM=59.708
PRIMER_RIGHT_36_TM=58.599
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=827
PRIMER_PAIR_37_PENALTY=1.693753
PRIMER_LEFT_37_PENALTY=0.292414
PRIMER_RIGHT_37_PENALTY=1.401339
PRIMER_LEFT_37_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_37_SEQUENCE=TTTCGATAGCGTCTTCCTCA
PRIMER_LEFT_37=704,20
PRIMER_RIGHT_37=1531,20
PRIMER_LEFT_37_TM=59.708
PRIMER_RIGHT_37_TM=58.599
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=828
PRIMER_PAIR_38_PENALTY=1.720871
PRIMER_LEFT_38_PENALTY=1.007644
PRIMER_RIGHT_38_PENALTY=0.713227
PRIMER_LEFT_38_SEQUENCE=GTTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_38_SEQUENCE=TTGGATCCCTTGCTTCTCAA
PRIMER_LEFT_38=812,21
PRIMER_RIGHT_38=1562,20
PRIMER_LEFT_38_TM=59.992
PRIMER_RIGHT_38_TM=60.713
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.4000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=751
PRIMER_PAIR_39_PENALTY=1.720871
PRIMER_LEFT_39_PENALTY=1.007644
PRIMER_RIGHT_39_PENALTY=0.713227
PRIMER_LEFT_39_SEQUENCE=AATGTTTGCGTTGATGTCTCC
PRIMER_RIGHT_39_SEQUENCE=TTGGATCCCTTGCTTCTCAA
PRIMER_LEFT_39=809,21
PRIMER_RIGHT_39=1562,20
PRIMER_LEFT_39_TM=59.992
PRIMER_RIGHT_39_TM=60.713
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=754
PRIMER_PAIR_40_PENALTY=1.721624
PRIMER_LEFT_40_PENALTY=0.516718
PRIMER_RIGHT_40_PENALTY=1.204906
PRIMER_LEFT_40_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_40_SEQUENCE=GGATCCCTTGCTTCTCAAGTC
PRIMER_LEFT_40=791,20
PRIMER_RIGHT_40=1560,21
PRIMER_LEFT_40_TM=60.517
PRIMER_RIGHT_40_TM=60.205
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=10.0000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=770
PRIMER_PAIR_41_PENALTY=1.760696
PRIMER_LEFT_41_PENALTY=1.007644
PRIMER_RIGHT_41_PENALTY=0.753052
PRIMER_LEFT_41_SEQUENCE=AATGTTTGCGTTGATGTCTCC
PRIMER_RIGHT_41_SEQUENCE=TTCAGGGCACAATTGAAAGA
PRIMER_LEFT_41=809,21
PRIMER_RIGHT_41=1418,20
PRIMER_LEFT_41_TM=59.992
PRIMER_RIGHT_41_TM=59.247
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=610
PRIMER_PAIR_42_PENALTY=1.766920
PRIMER_LEFT_42_PENALTY=0.292414
PRIMER_RIGHT_42_PENALTY=1.474506
PRIMER_LEFT_42_SEQUENCE=GCAATGGGTAAAGCCAGAAG
PRIMER_RIGHT_42_SEQUENCE=GGCACAATTGAAAGACGTGG
PRIMER_LEFT_42=704,20
PRIMER_RIGHT_42=1413,20
PRIMER_LEFT_42_TM=59.708
PRIMER_RIGHT_42_TM=61.475
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=9.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=710
PRIMER_PAIR_43_PENALTY=1.771640
PRIMER_LEFT_43_PENALTY=0.516718
PRIMER_RIGHT_43_PENALTY=1.254922
PRIMER_LEFT_43_SEQUENCE=CATGTCCATGTCAAAGGCAA
PRIMER_RIGHT_43_SEQUENCE=TTTCAGGGCACAATTGAAAG
PRIMER_LEFT_43=791,20
PRIMER_RIGHT_43=1419,20
PRIMER_LEFT_43_TM=60.517
PRIMER_RIGHT_43_TM=58.745
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.0000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=629
PRIMER_PAIR_44_PENALTY=1.799355
PRIMER_LEFT_44_PENALTY=1.428318
PRIMER_RIGHT_44_PENALTY=0.371037
PRIMER_LEFT_44_SEQUENCE=ATGTTTGCGTTGATGTCTCC
PRIMER_RIGHT_44_SEQUENCE=ATTGGATCCCTTGCTTCTCA
PRIMER_LEFT_44=810,20
PRIMER_RIGHT_44=1563,20
PRIMER_LEFT_44_TM=58.572
PRIMER_RIGHT_44_TM=59.629
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=754
PRIMER_PAIR_45_PENALTY=1.802296
PRIMER_LEFT_45_PENALTY=1.007644
PRIMER_RIGHT_45_PENALTY=0.794652
PRIMER_LEFT_45_SEQUENCE=GTTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_45_SEQUENCE=GCCAATTTGCAGGTTTTTCT
PRIMER_LEFT_45=812,21
PRIMER_RIGHT_45=1603,20
PRIMER_LEFT_45_TM=59.992
PRIMER_RIGHT_45_TM=59.205
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.4000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=792
PRIMER_PAIR_46_PENALTY=1.802296
PRIMER_LEFT_46_PENALTY=1.007644
PRIMER_RIGHT_46_PENALTY=0.794652
PRIMER_LEFT_46_SEQUENCE=GTTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_46_SEQUENCE=AGCCAATTTGCAGGTTTTTC
PRIMER_LEFT_46=812,21
PRIMER_RIGHT_46=1604,20
PRIMER_LEFT_46_TM=59.992
PRIMER_RIGHT_46_TM=59.205
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.4000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=793
PRIMER_PAIR_47_PENALTY=1.802296
PRIMER_LEFT_47_PENALTY=1.007644
PRIMER_RIGHT_47_PENALTY=0.794652
PRIMER_LEFT_47_SEQUENCE=GTTTGCGTTGATGTCTCCATT
PRIMER_RIGHT_47_SEQUENCE=GAGCCAATTTGCAGGTTTTT
PRIMER_LEFT_47=812,21
PRIMER_RIGHT_47=1605,20
PRIMER_LEFT_47_TM=59.992
PRIMER_RIGHT_47_TM=59.205
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.4000
PRIMER_RIGHT_47_END_STABILITY=7.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=794
PRIMER_PAIR_48_PENALTY=1.802296
PRIMER_LEFT_48_PENALTY=1.007644
PRIMER_RIGHT_48_PENALTY=0.794652
PRIMER_LEFT_48_SEQUENCE=AATGTTTGCGTTGATGTCTCC
PRIMER_RIGHT_48_SEQUENCE=GCCAATTTGCAGGTTTTTCT
PRIMER_LEFT_48=809,21
PRIMER_RIGHT_48=1603,20
PRIMER_LEFT_48_TM=59.992
PRIMER_RIGHT_48_TM=59.205
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=795
PRIMER_PAIR_49_PENALTY=1.802296
PRIMER_LEFT_49_PENALTY=1.007644
PRIMER_RIGHT_49_PENALTY=0.794652
PRIMER_LEFT_49_SEQUENCE=AATGTTTGCGTTGATGTCTCC
PRIMER_RIGHT_49_SEQUENCE=AGCCAATTTGCAGGTTTTTC
PRIMER_LEFT_49=809,21
PRIMER_RIGHT_49=1604,20
PRIMER_LEFT_49_TM=59.992
PRIMER_RIGHT_49_TM=59.205
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=796
=
SEQUENCE_ID=MAGIC.105_4_13070741_13071514_zu-0_can-0_63148_187412_TRUE
SEQUENCE_TARGET=950,873
SEQUENCE_TEMPLATE=GAGAGTAACGCATATNTATANGTGATGAGTCCCCAATGCAGACAAATGGCTTTCTTTTTTTNAAAGGCAAAATTGATTGGAGTGACTTAAAAANNNAAAANAAACTTCAAGAAAANATGTTTATACCAANATTACCCTCGTTATTGTCGTGGTAATTACAGGGGTATGCCACTCAGTAATGGCTGGNGTCGCGAAACTCGTGAGCANTGCGAGTCTTTTAAGATAAATGCGCCGCTGATTTCATTCCGTTGGGTGGCGAGTGAGATCCTCGTGACTTACATTAGTTTTTTTCTCTAACTTATTTATTAGCTTTCATANCAACATAGTTATTGCTAAGGTCGGGTTTGGAATGATTTCTTTTAATNGTTTTTNNNTAANTTATAAAACGGCAATGTTTCTATTTTGCATGGTGTACACATGTGAAAGAACACGTCTATCTACTACANTCAAGTACGTATGTCCAATANATTCACTAGAGAGCTTTTAACAGCTGAAAAAGTNAATATTCTTTATCTAGTCAAGTTTATATTCTCGTGTAAGCTATGATGAGACTGACTCTATCAATTCTTTGGCGATTTTGGATTCTAGTGTGGTCAGTTTTGAGCTTCTTTTGCATGTTATGAGAATATTTCTCAATGAACTTGAAATGGAATATATTTGTTGGGNAAATTATACTTCTAAACATATAATGCTAATAAGATTANTNAAATCACCAAATAATAAGATGGTATAACATACTTTGTTAGCTAGAAAAAATCGAATGACTTCCATGTGATATATTTGAATATGCATATTCTAANAAATGCCAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTGATGTGAAGACTTGGAAATCTTTGATGGNTATCTTCGAAATTGATTAGTTTCTAATTTTAAATATCCTTAAAANACCAATTAGCTCTAGAAATTATCGACGTAAAAAGAGGTCTAAAAATTATCGATTNANTAGACNGNGGNAGTGGATAGTCAACTAAAAAGAGTTAAANAGATTCTTTAGAATCGAATCGAACGGTTGTAATTTGTAAACGNCCTTATCAAAATGGTTAACAACTATACTATTAGTTTGCCTAACCAGTAGTTGACTTTTTATTATTGTTATTTCCTTTTNNGGTTTTTTATACACATGAGACTANAATTAGGAGGAGAATTTCACTGCTCTTAGCCGCCATACTTTAGTAATNGCGGATCTTCGATGATGTATCGATTATGATTTTGTAGGGNTTGTGTTTTATNAATGATTAAACATTTAAACTAAACCAAAGCGACAAAGAAGATTTGTGAGGGTGGTTTCGATAAGGATTTTGTAGGGTTTGTCATTTGTCATTTGTGAATGATTAAACTAAACCAAAGCGACTAAAAAGATTTGAGAGGTTAGTTTAGTAAAATTGATTTTATAGGCCAATGAAAAAGGGAAGAAGAGAAGGGTTTTATGGGCTAAAAGAGAGCGCACGTGTGCATCGCATTCTCTTCTTCTTTTGCTTTTGTTGAGTTGCGTAATGACTTTTACGTGTATCCCTCGTGCGTGTGTACGAATGCGACCCTTTACCTTTCTTTGTCTCTTGAGAAGTTGGATATTCACNTTTACATTATTTAAACACTATATCAGTAACATAAATGAAGTATAGAAAAACCTATCAAACATATTTTGAATNTTTTTATTCTAATGCTTTGAAAAAGAAAAAGAAAANATATATATATCATAATACTTAAGTACTATGGAATACGTAATNTACAATTATTTTTGTATGTGAATATCCAATTTCATTTGCNATGTTAATATACATTTTTTAGATTTTATCATCAATCATGGNAAAATATAGTCTTTTGAATAGATGAGCATATCCTTANTAGAAGCCACTCNAANCAATCATGGATGTCTTCCAATGACCATCTAACATTTAAGTCATTATTGGAAACTTAAAGAAGTTGTATAACAAAATACTTAGATGTTATTATGAGGATGCTCTTAAATTAGTATATGTCAAGTGTTGTTATTGAGAATATAGCAATTACTTCTCTCTCTATTATGCTCATGTTGGATTGTGAATCCTTAAGAAAAATTCTTAAGACTGATATTTATAAGNNAAAAAAACTGAACATGGTTCAGATACCGGGTTTCAAAACATTATGGTGTTAAGTCCATACAAATGTACTTGTTTTAAAGTAGAAAACATGTGTGGTGTTGGTCAAGTTTTTCTATTACATCCTATCAAAGCATGTACACAATTTTAGGTAGACGTAATAATGTTGTTAGTATTCTCTTNACGGAAGATGTGTGTGATGCGTCCTATCACATATTNTCATTGCAAAGTGTGTTAGCTCANNATGNACATGGTGGTTTAGCTCTTTATGTAGATTGTTAGGATCTGGTTTGAAAAAATAAATAATAGTTACTTAACATTCATACNCAAAAAAATAATAAAGAATAGTTGATAATATTTCATGAAAATCGATTTGTTTTGGTGGACATACATCGTCGTCTCTACTCTATTGTCTCGATCGCAAGTAATATGACAAATAAAGCAGCAGCCNTAGCNATAAAACATGTGACTACTAAAATAGGAGGATTTATCTTTACTTGACNAACTATAAATTAAATATCTACTCTTTTACCTTGACTTTGAAAATTACTTTTTAAGATTGCCTTTTACAATTATGTGGAATAGCGGCNCACATTTACGTAAATTTAACTTCAATTTANAACANTNNTTTATTCATTTTCNTCATAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_8566433_8567012_ct-1_ler-0_4975_230042_TRUE
SEQUENCE_TARGET=950,679
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNTTTTTTNATANAAAAAATAGNANNTGTNCANTAATAGAGAAATCAATCATCTATAATAAGCAAATGANGTCAGACTACAGAAGACGATAGAAGAATCATCTTTCTCCACCGTTGTTTTCTAGAGAACTACTTGCGGACCACTTATTAATTTTAGGGATATGATATTGANAGCGACTAACCAAATCGATATGAACTAATATANAATGAAAATAAACAAAACTGTATATATATTATTTAAACTGTGAAAGTTCAATTTCATTCACGTCACTGAATTCAAAATATCAAACGATATGAATTTTATCACCTTTTCGTTTTCAGCAAAATTGGATTTAGAAATGTAATAGTATTTCCATATAACAAAACGTTTTTATTAAACATTAACGTTAATGGCACANACCGAAGAATATCCTAAAAAATTGCGCCACCTGGTTCAAGTCGCTATGGATGGGATGGTGTGTCGTCTGCTCATCAACGTACATTTANCGACTANTCGGGATCTATATCTTCTAGAGGNTTTTAGNCTTTAGCCCAACATTAAAAANAAAAAAAACTGNCGGTTACGTACGTTGTTTTCAGNTTTCCCTTAACAGAACGAGCATTTCATTTATTGGTTTTCTTAAAGAAATTTTCTGTCATTGTCAATGTCGTGGACCAAAACCATTAAACTGATATTGAGCTTTTATATGNTTTTTTGTTAGTTTGTAGGNAAATTGAACTTTTACTTGTTAGGTTATAAGTTGACGATNATTTAGCACGTCGTTTAGTGATTAATCTATTGATGGTGTCCATTGTCCAAGCTNNNNNNCCATACAAAAATCTATAGGTGGTGTCCGTCATTCCACTAAGTANGATTTTTTGTTTGAATCATTTCTATATAGTAAATTACATTCCATATAGTTGTAGAAAACNCTTTATTTANTTTGTATTTCCAGTATTTTAAGTTTCTCTTTTATAGANCATAGTTTCAGANAAATTTNGTTTTCTTTCGNCTATNTTTN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_17400274_17400284_sf-2_ler-0_775151_142468_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGTCCAGACCTATTTCTCGCAACAATAATCAGGTNNATTGGACTTCTACACCACGATAGAAGNTCGAACANAATCCACAATCTCCTTCTTGAGCGACATAAGAANNGATTCTTTGGAAGCTTTTATTATGGTGTAGAAGTCACATTCTTGTGTTTTATTTNGTTGCCCTAAGNNCTAGTACNGNGTAACTGTAAATGTTGCTCTCTTTGGATAGNNNAGATCTNAACTTTGAATGAAATTCATTAGNTTTCTNATATATTANNATAAGTAAACGACTAGATCTAATTTTAACCTTGCTTTTGGAGTGAATATATAAGCTTGTTTCCAGAATAGTGAGTCGATTTCGAAATATTNNACNANATTTAAATGATATCATAAATATTCTTATAACATCATAACTTAAGGTCANNGANCCAATAAAATGAAAACAAAACCAAANATCGCACTCCAAATAACAAAATAAANATACATAATTATTTCTCGTCATGGCTAACNTCAGCNTCACCAGANAGTTCTTCNAGGGACTTNCCNTTNGGCTCTGGGACAAGGAAGGTGAAGAGCATACCGATAAAGTTAAGAACACCAAGCATGATCAATGAGTTCTTAACNCCGATGCCTGGTGGGTATCCTGCNTCTACCTTGGCCTTGTCTTGTGATTGAGCCGCATATAGGAACCCAAAGGCTCCAACAATGGCTCCAGCCTTACCNGCCGCGGCTGATATTCCNTGACATGTNGACCTTAGCCTGGCNGGNAANATCTCAGCAGGGACAATAAANGTGGTTGCATTTGGACCAAAATTGGCNAAGAAGAAAGTNAGAGAGTACATNANCACNAATCCNATACGGTTNTCTGGTTNGATCCAGTGGTTGTAAGGGAANGCAATGGCAAACATAAACACGGTCATCATGAAAAANNNNNNNNNNNNNNNNNNNNNNCTTCCAATGNTATCAATAAACGCAACTGTGAACCAGTAGCCTGGGACNGTACTGCAAAGGGCGATAAGAGTNTGAGCCCTAGCAATCCTGAAAACNTCATGGGTGGCGTTCATGGTGGCTGCCTTTGGGATCCATCCGATGGCCGAGAAAATATCCTTCTGGAACAAGTTTTGGCTGTAGAAGGCAATGTCAAGCAAAAACCATGTGGAGGTAGTTCCAAGGAGATGAAGCCCATGGCGTCTAAGGAATTCCTTGGAGAACAAGCCATAGTTTTGTNTGGGGTCTTTGACGTCATCCTCCACCCTTTCCTCAAGCTCGATATCTGTTTGTAAGACCTTGGACATGTCGGCTGTGGCTTGTTTGATGTTCTTGGCAACCAAAGCGGTGTAACGGGCAGTTTCAGGCATCTTCATACGCCAGTAGTAAGTCAAAGCTGCGGGTAAAGCACCAAACATGACGATGATTCGCCAAATGTAGTCAACTTGAGGAGGCGTTGAGAGGGCCCTGTTTACTGCATATGTTGGAGCTGGGAACTTTTTGTCGAATATAGAAGATACTGCGAGTGCCACGAAACCTCCAGCCAAGATACCGACACCTTGCATGGCGAAGACAGCTGCGATGAAAGCCCCACGGGTCTTCTTGTTTGCGTATTCAGACATGATGGTGGCAGAAAGTGGGTAGTCACCTCCAATACCAAATCCCANCCAAAACCTACNTAATNGTNTATATNNANAAAANNTNNNNNNNNNNNNNNNNNNNNNATCAGTTCGAAGCGTGACTAACTTTTAAAACTTGCATTCGTTATNAGATTATTGGTTAATCANTGTGTATATATANTATTGTAATTNGATGAAAATTNTACCTGAAGAAGCAAAGGGTGGTCATGACACCCTTGGCTTNGNG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.258434
PRIMER_LEFT_0_PENALTY=0.229181
PRIMER_RIGHT_0_PENALTY=0.029253
PRIMER_LEFT_0_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_0_SEQUENCE=CCTCCACATGGTTTTTGCTT
PRIMER_LEFT_0=707,20
PRIMER_RIGHT_0=1317,20
PRIMER_LEFT_0_TM=60.229
PRIMER_RIGHT_0_TM=59.971
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=611
PRIMER_PAIR_1_PENALTY=0.269110
PRIMER_LEFT_1_PENALTY=0.239857
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_1_SEQUENCE=CCTCCACATGGTTTTTGCTT
PRIMER_LEFT_1=811,20
PRIMER_RIGHT_1=1317,20
PRIMER_LEFT_1_TM=60.240
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=507
PRIMER_PAIR_2_PENALTY=0.317427
PRIMER_LEFT_2_PENALTY=0.229181
PRIMER_RIGHT_2_PENALTY=0.088247
PRIMER_LEFT_2_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_2_SEQUENCE=TCCACATGGTTTTTGCTTGA
PRIMER_LEFT_2=707,20
PRIMER_RIGHT_2=1315,20
PRIMER_LEFT_2_TM=60.229
PRIMER_RIGHT_2_TM=60.088
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=609
PRIMER_PAIR_3_PENALTY=0.317427
PRIMER_LEFT_3_PENALTY=0.229181
PRIMER_RIGHT_3_PENALTY=0.088247
PRIMER_LEFT_3_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_3_SEQUENCE=TGGTTGCCAAGAACATCAAA
PRIMER_LEFT_3=707,20
PRIMER_RIGHT_3=1485,20
PRIMER_LEFT_3_TM=60.229
PRIMER_RIGHT_3_TM=60.088
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=779
PRIMER_PAIR_4_PENALTY=0.317427
PRIMER_LEFT_4_PENALTY=0.229181
PRIMER_RIGHT_4_PENALTY=0.088247
PRIMER_LEFT_4_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_4_SEQUENCE=TTGGTTGCCAAGAACATCAA
PRIMER_LEFT_4=707,20
PRIMER_RIGHT_4=1486,20
PRIMER_LEFT_4_TM=60.229
PRIMER_RIGHT_4_TM=60.088
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=780
PRIMER_PAIR_5_PENALTY=0.317427
PRIMER_LEFT_5_PENALTY=0.229181
PRIMER_RIGHT_5_PENALTY=0.088247
PRIMER_LEFT_5_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_5_SEQUENCE=TTTGGTTGCCAAGAACATCA
PRIMER_LEFT_5=707,20
PRIMER_RIGHT_5=1487,20
PRIMER_LEFT_5_TM=60.229
PRIMER_RIGHT_5_TM=60.088
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=781
PRIMER_PAIR_6_PENALTY=0.328103
PRIMER_LEFT_6_PENALTY=0.239857
PRIMER_RIGHT_6_PENALTY=0.088247
PRIMER_LEFT_6_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_6_SEQUENCE=TCCACATGGTTTTTGCTTGA
PRIMER_LEFT_6=811,20
PRIMER_RIGHT_6=1315,20
PRIMER_LEFT_6_TM=60.240
PRIMER_RIGHT_6_TM=60.088
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=505
PRIMER_PAIR_7_PENALTY=0.328103
PRIMER_LEFT_7_PENALTY=0.239857
PRIMER_RIGHT_7_PENALTY=0.088247
PRIMER_LEFT_7_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_7_SEQUENCE=TGGTTGCCAAGAACATCAAA
PRIMER_LEFT_7=811,20
PRIMER_RIGHT_7=1485,20
PRIMER_LEFT_7_TM=60.240
PRIMER_RIGHT_7_TM=60.088
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=675
PRIMER_PAIR_8_PENALTY=0.328103
PRIMER_LEFT_8_PENALTY=0.239857
PRIMER_RIGHT_8_PENALTY=0.088247
PRIMER_LEFT_8_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_8_SEQUENCE=TTTGGTTGCCAAGAACATCA
PRIMER_LEFT_8=811,20
PRIMER_RIGHT_8=1487,20
PRIMER_LEFT_8_TM=60.240
PRIMER_RIGHT_8_TM=60.088
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=677
PRIMER_PAIR_9_PENALTY=0.337975
PRIMER_LEFT_9_PENALTY=0.308722
PRIMER_RIGHT_9_PENALTY=0.029253
PRIMER_LEFT_9_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_9_SEQUENCE=CCTCCACATGGTTTTTGCTT
PRIMER_LEFT_9=810,20
PRIMER_RIGHT_9=1317,20
PRIMER_LEFT_9_TM=59.691
PRIMER_RIGHT_9_TM=59.971
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=508
PRIMER_PAIR_10_PENALTY=0.338124
PRIMER_LEFT_10_PENALTY=0.308871
PRIMER_RIGHT_10_PENALTY=0.029253
PRIMER_LEFT_10_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_10_SEQUENCE=CCTCCACATGGTTTTTGCTT
PRIMER_LEFT_10=709,20
PRIMER_RIGHT_10=1317,20
PRIMER_LEFT_10_TM=59.691
PRIMER_RIGHT_10_TM=59.971
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=609
PRIMER_PAIR_11_PENALTY=0.346462
PRIMER_LEFT_11_PENALTY=0.229181
PRIMER_RIGHT_11_PENALTY=0.117281
PRIMER_LEFT_11_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_11_SEQUENCE=TTTACCCGCAGCTTTGACTT
PRIMER_LEFT_11=707,20
PRIMER_RIGHT_11=1550,20
PRIMER_LEFT_11_TM=60.229
PRIMER_RIGHT_11_TM=59.883
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=844
PRIMER_PAIR_12_PENALTY=0.349425
PRIMER_LEFT_12_PENALTY=0.229181
PRIMER_RIGHT_12_PENALTY=0.120245
PRIMER_LEFT_12_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_12_SEQUENCE=TCATCGTCATGTTTGGTGCT
PRIMER_LEFT_12=707,20
PRIMER_RIGHT_12=1569,20
PRIMER_LEFT_12_TM=60.229
PRIMER_RIGHT_12_TM=60.120
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=863
PRIMER_PAIR_13_PENALTY=0.357138
PRIMER_LEFT_13_PENALTY=0.239857
PRIMER_RIGHT_13_PENALTY=0.117281
PRIMER_LEFT_13_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_13_SEQUENCE=TTTACCCGCAGCTTTGACTT
PRIMER_LEFT_13=811,20
PRIMER_RIGHT_13=1550,20
PRIMER_LEFT_13_TM=60.240
PRIMER_RIGHT_13_TM=59.883
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=740
PRIMER_PAIR_14_PENALTY=0.360101
PRIMER_LEFT_14_PENALTY=0.239857
PRIMER_RIGHT_14_PENALTY=0.120245
PRIMER_LEFT_14_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_14_SEQUENCE=TCATCGTCATGTTTGGTGCT
PRIMER_LEFT_14=811,20
PRIMER_RIGHT_14=1569,20
PRIMER_LEFT_14_TM=60.240
PRIMER_RIGHT_14_TM=60.120
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=759
PRIMER_PAIR_15_PENALTY=0.362921
PRIMER_LEFT_15_PENALTY=0.229181
PRIMER_RIGHT_15_PENALTY=0.133741
PRIMER_LEFT_15_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_15_SEQUENCE=AGGTGTCGGTATCTTGGCTG
PRIMER_LEFT_15=707,20
PRIMER_RIGHT_15=1703,20
PRIMER_LEFT_15_TM=60.229
PRIMER_RIGHT_15_TM=60.134
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=997
PRIMER_PAIR_16_PENALTY=0.373597
PRIMER_LEFT_16_PENALTY=0.239857
PRIMER_RIGHT_16_PENALTY=0.133741
PRIMER_LEFT_16_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_16_SEQUENCE=AGGTGTCGGTATCTTGGCTG
PRIMER_LEFT_16=811,20
PRIMER_RIGHT_16=1703,20
PRIMER_LEFT_16_TM=60.240
PRIMER_RIGHT_16_TM=60.134
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=893
PRIMER_PAIR_17_PENALTY=0.396968
PRIMER_LEFT_17_PENALTY=0.308722
PRIMER_RIGHT_17_PENALTY=0.088247
PRIMER_LEFT_17_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_17_SEQUENCE=TCCACATGGTTTTTGCTTGA
PRIMER_LEFT_17=810,20
PRIMER_RIGHT_17=1315,20
PRIMER_LEFT_17_TM=59.691
PRIMER_RIGHT_17_TM=60.088
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=506
PRIMER_PAIR_18_PENALTY=0.396968
PRIMER_LEFT_18_PENALTY=0.308722
PRIMER_RIGHT_18_PENALTY=0.088247
PRIMER_LEFT_18_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_18_SEQUENCE=TGGTTGCCAAGAACATCAAA
PRIMER_LEFT_18=810,20
PRIMER_RIGHT_18=1485,20
PRIMER_LEFT_18_TM=59.691
PRIMER_RIGHT_18_TM=60.088
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=676
PRIMER_PAIR_19_PENALTY=0.396968
PRIMER_LEFT_19_PENALTY=0.308722
PRIMER_RIGHT_19_PENALTY=0.088247
PRIMER_LEFT_19_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_19_SEQUENCE=TTGGTTGCCAAGAACATCAA
PRIMER_LEFT_19=810,20
PRIMER_RIGHT_19=1486,20
PRIMER_LEFT_19_TM=59.691
PRIMER_RIGHT_19_TM=60.088
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=677
PRIMER_PAIR_20_PENALTY=0.396968
PRIMER_LEFT_20_PENALTY=0.308722
PRIMER_RIGHT_20_PENALTY=0.088247
PRIMER_LEFT_20_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_20_SEQUENCE=TTTGGTTGCCAAGAACATCA
PRIMER_LEFT_20=810,20
PRIMER_RIGHT_20=1487,20
PRIMER_LEFT_20_TM=59.691
PRIMER_RIGHT_20_TM=60.088
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=678
PRIMER_PAIR_21_PENALTY=0.397118
PRIMER_LEFT_21_PENALTY=0.308871
PRIMER_RIGHT_21_PENALTY=0.088247
PRIMER_LEFT_21_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_21_SEQUENCE=TCCACATGGTTTTTGCTTGA
PRIMER_LEFT_21=709,20
PRIMER_RIGHT_21=1315,20
PRIMER_LEFT_21_TM=59.691
PRIMER_RIGHT_21_TM=60.088
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=607
PRIMER_PAIR_22_PENALTY=0.397118
PRIMER_LEFT_22_PENALTY=0.308871
PRIMER_RIGHT_22_PENALTY=0.088247
PRIMER_LEFT_22_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_22_SEQUENCE=TGGTTGCCAAGAACATCAAA
PRIMER_LEFT_22=709,20
PRIMER_RIGHT_22=1485,20
PRIMER_LEFT_22_TM=59.691
PRIMER_RIGHT_22_TM=60.088
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=777
PRIMER_PAIR_23_PENALTY=0.397118
PRIMER_LEFT_23_PENALTY=0.308871
PRIMER_RIGHT_23_PENALTY=0.088247
PRIMER_LEFT_23_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_23_SEQUENCE=TTGGTTGCCAAGAACATCAA
PRIMER_LEFT_23=709,20
PRIMER_RIGHT_23=1486,20
PRIMER_LEFT_23_TM=59.691
PRIMER_RIGHT_23_TM=60.088
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=778
PRIMER_PAIR_24_PENALTY=0.397118
PRIMER_LEFT_24_PENALTY=0.308871
PRIMER_RIGHT_24_PENALTY=0.088247
PRIMER_LEFT_24_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_24_SEQUENCE=TTTGGTTGCCAAGAACATCA
PRIMER_LEFT_24=709,20
PRIMER_RIGHT_24=1487,20
PRIMER_LEFT_24_TM=59.691
PRIMER_RIGHT_24_TM=60.088
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=779
PRIMER_PAIR_25_PENALTY=0.426003
PRIMER_LEFT_25_PENALTY=0.308722
PRIMER_RIGHT_25_PENALTY=0.117281
PRIMER_LEFT_25_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_25_SEQUENCE=TTTACCCGCAGCTTTGACTT
PRIMER_LEFT_25=810,20
PRIMER_RIGHT_25=1550,20
PRIMER_LEFT_25_TM=59.691
PRIMER_RIGHT_25_TM=59.883
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.9000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=741
PRIMER_PAIR_26_PENALTY=0.426152
PRIMER_LEFT_26_PENALTY=0.308871
PRIMER_RIGHT_26_PENALTY=0.117281
PRIMER_LEFT_26_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_26_SEQUENCE=TTTACCCGCAGCTTTGACTT
PRIMER_LEFT_26=709,20
PRIMER_RIGHT_26=1550,20
PRIMER_LEFT_26_TM=59.691
PRIMER_RIGHT_26_TM=59.883
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=842
PRIMER_PAIR_27_PENALTY=0.428966
PRIMER_LEFT_27_PENALTY=0.308722
PRIMER_RIGHT_27_PENALTY=0.120245
PRIMER_LEFT_27_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_27_SEQUENCE=TCATCGTCATGTTTGGTGCT
PRIMER_LEFT_27=810,20
PRIMER_RIGHT_27=1569,20
PRIMER_LEFT_27_TM=59.691
PRIMER_RIGHT_27_TM=60.120
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=760
PRIMER_PAIR_28_PENALTY=0.429116
PRIMER_LEFT_28_PENALTY=0.308871
PRIMER_RIGHT_28_PENALTY=0.120245
PRIMER_LEFT_28_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_28_SEQUENCE=TCATCGTCATGTTTGGTGCT
PRIMER_LEFT_28=709,20
PRIMER_RIGHT_28=1569,20
PRIMER_LEFT_28_TM=59.691
PRIMER_RIGHT_28_TM=60.120
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=861
PRIMER_PAIR_29_PENALTY=0.440987
PRIMER_LEFT_29_PENALTY=0.229181
PRIMER_RIGHT_29_PENALTY=0.211806
PRIMER_LEFT_29_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_29_SEQUENCE=ATCGAGCTTGAGGAAAGGGT
PRIMER_LEFT_29=707,20
PRIMER_RIGHT_29=1426,20
PRIMER_LEFT_29_TM=60.229
PRIMER_RIGHT_29_TM=60.212
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=9.1000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=720
PRIMER_PAIR_30_PENALTY=0.442462
PRIMER_LEFT_30_PENALTY=0.308722
PRIMER_RIGHT_30_PENALTY=0.133741
PRIMER_LEFT_30_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_30_SEQUENCE=AGGTGTCGGTATCTTGGCTG
PRIMER_LEFT_30=810,20
PRIMER_RIGHT_30=1703,20
PRIMER_LEFT_30_TM=59.691
PRIMER_RIGHT_30_TM=60.134
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=894
PRIMER_PAIR_31_PENALTY=0.442612
PRIMER_LEFT_31_PENALTY=0.308871
PRIMER_RIGHT_31_PENALTY=0.133741
PRIMER_LEFT_31_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_31_SEQUENCE=AGGTGTCGGTATCTTGGCTG
PRIMER_LEFT_31=709,20
PRIMER_RIGHT_31=1703,20
PRIMER_LEFT_31_TM=59.691
PRIMER_RIGHT_31_TM=60.134
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=995
PRIMER_PAIR_32_PENALTY=0.448732
PRIMER_LEFT_32_PENALTY=0.229181
PRIMER_RIGHT_32_PENALTY=0.219551
PRIMER_LEFT_32_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_32_SEQUENCE=CAAAAAGTTCCCAGCTCCAA
PRIMER_LEFT_32=707,20
PRIMER_RIGHT_32=1646,20
PRIMER_LEFT_32_TM=60.229
PRIMER_RIGHT_32_TM=60.220
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=940
PRIMER_PAIR_33_PENALTY=0.451662
PRIMER_LEFT_33_PENALTY=0.239857
PRIMER_RIGHT_33_PENALTY=0.211806
PRIMER_LEFT_33_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_33_SEQUENCE=ATCGAGCTTGAGGAAAGGGT
PRIMER_LEFT_33=811,20
PRIMER_RIGHT_33=1426,20
PRIMER_LEFT_33_TM=60.240
PRIMER_RIGHT_33_TM=60.212
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=9.1000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=616
PRIMER_PAIR_34_PENALTY=0.459408
PRIMER_LEFT_34_PENALTY=0.239857
PRIMER_RIGHT_34_PENALTY=0.219551
PRIMER_LEFT_34_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_34_SEQUENCE=CAAAAAGTTCCCAGCTCCAA
PRIMER_LEFT_34=811,20
PRIMER_RIGHT_34=1646,20
PRIMER_LEFT_34_TM=60.240
PRIMER_RIGHT_34_TM=60.220
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=836
PRIMER_PAIR_35_PENALTY=0.517985
PRIMER_LEFT_35_PENALTY=0.229181
PRIMER_RIGHT_35_PENALTY=0.288804
PRIMER_LEFT_35_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_35_SEQUENCE=AAAAGTTCCCAGCTCCAACA
PRIMER_LEFT_35=707,20
PRIMER_RIGHT_35=1644,20
PRIMER_LEFT_35_TM=60.229
PRIMER_RIGHT_35_TM=59.711
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=938
PRIMER_PAIR_36_PENALTY=0.517985
PRIMER_LEFT_36_PENALTY=0.229181
PRIMER_RIGHT_36_PENALTY=0.288804
PRIMER_LEFT_36_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_36_SEQUENCE=ACAAAAAGTTCCCAGCTCCA
PRIMER_LEFT_36=707,20
PRIMER_RIGHT_36=1647,20
PRIMER_LEFT_36_TM=60.229
PRIMER_RIGHT_36_TM=59.711
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=941
PRIMER_PAIR_37_PENALTY=0.520527
PRIMER_LEFT_37_PENALTY=0.308722
PRIMER_RIGHT_37_PENALTY=0.211806
PRIMER_LEFT_37_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_37_SEQUENCE=ATCGAGCTTGAGGAAAGGGT
PRIMER_LEFT_37=810,20
PRIMER_RIGHT_37=1426,20
PRIMER_LEFT_37_TM=59.691
PRIMER_RIGHT_37_TM=60.212
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=9.1000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=617
PRIMER_PAIR_38_PENALTY=0.520677
PRIMER_LEFT_38_PENALTY=0.308871
PRIMER_RIGHT_38_PENALTY=0.211806
PRIMER_LEFT_38_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_38_SEQUENCE=ATCGAGCTTGAGGAAAGGGT
PRIMER_LEFT_38=709,20
PRIMER_RIGHT_38=1426,20
PRIMER_LEFT_38_TM=59.691
PRIMER_RIGHT_38_TM=60.212
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=9.1000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=718
PRIMER_PAIR_39_PENALTY=0.528273
PRIMER_LEFT_39_PENALTY=0.308722
PRIMER_RIGHT_39_PENALTY=0.219551
PRIMER_LEFT_39_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_39_SEQUENCE=CAAAAAGTTCCCAGCTCCAA
PRIMER_LEFT_39=810,20
PRIMER_RIGHT_39=1646,20
PRIMER_LEFT_39_TM=59.691
PRIMER_RIGHT_39_TM=60.220
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=837
PRIMER_PAIR_40_PENALTY=0.528422
PRIMER_LEFT_40_PENALTY=0.308871
PRIMER_RIGHT_40_PENALTY=0.219551
PRIMER_LEFT_40_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_40_SEQUENCE=CAAAAAGTTCCCAGCTCCAA
PRIMER_LEFT_40=709,20
PRIMER_RIGHT_40=1646,20
PRIMER_LEFT_40_TM=59.691
PRIMER_RIGHT_40_TM=60.220
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=938
PRIMER_PAIR_41_PENALTY=0.528661
PRIMER_LEFT_41_PENALTY=0.239857
PRIMER_RIGHT_41_PENALTY=0.288804
PRIMER_LEFT_41_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_41_SEQUENCE=AAAAGTTCCCAGCTCCAACA
PRIMER_LEFT_41=811,20
PRIMER_RIGHT_41=1644,20
PRIMER_LEFT_41_TM=60.240
PRIMER_RIGHT_41_TM=59.711
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=834
PRIMER_PAIR_42_PENALTY=0.528661
PRIMER_LEFT_42_PENALTY=0.239857
PRIMER_RIGHT_42_PENALTY=0.288804
PRIMER_LEFT_42_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_42_SEQUENCE=ACAAAAAGTTCCCAGCTCCA
PRIMER_LEFT_42=811,20
PRIMER_RIGHT_42=1647,20
PRIMER_LEFT_42_TM=60.240
PRIMER_RIGHT_42_TM=59.711
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=837
PRIMER_PAIR_43_PENALTY=0.543274
PRIMER_LEFT_43_PENALTY=0.229181
PRIMER_RIGHT_43_PENALTY=0.314094
PRIMER_LEFT_43_SEQUENCE=CTCTGGGACAAGGAAGGTGA
PRIMER_RIGHT_43_SEQUENCE=AGGAAAGGGTGGAGGATGAC
PRIMER_LEFT_43=707,20
PRIMER_RIGHT_43=1416,20
PRIMER_LEFT_43_TM=60.229
PRIMER_RIGHT_43_TM=60.314
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=710
PRIMER_PAIR_44_PENALTY=0.553950
PRIMER_LEFT_44_PENALTY=0.239857
PRIMER_RIGHT_44_PENALTY=0.314094
PRIMER_LEFT_44_SEQUENCE=TGGCCTTGTCTTGTGATTGA
PRIMER_RIGHT_44_SEQUENCE=AGGAAAGGGTGGAGGATGAC
PRIMER_LEFT_44=811,20
PRIMER_RIGHT_44=1416,20
PRIMER_LEFT_44_TM=60.240
PRIMER_RIGHT_44_TM=60.314
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=6.3000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=606
PRIMER_PAIR_45_PENALTY=0.597526
PRIMER_LEFT_45_PENALTY=0.308722
PRIMER_RIGHT_45_PENALTY=0.288804
PRIMER_LEFT_45_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_45_SEQUENCE=AAAAGTTCCCAGCTCCAACA
PRIMER_LEFT_45=810,20
PRIMER_RIGHT_45=1644,20
PRIMER_LEFT_45_TM=59.691
PRIMER_RIGHT_45_TM=59.711
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=835
PRIMER_PAIR_46_PENALTY=0.597526
PRIMER_LEFT_46_PENALTY=0.308722
PRIMER_RIGHT_46_PENALTY=0.288804
PRIMER_LEFT_46_SEQUENCE=TTGGCCTTGTCTTGTGATTG
PRIMER_RIGHT_46_SEQUENCE=ACAAAAAGTTCCCAGCTCCA
PRIMER_LEFT_46=810,20
PRIMER_RIGHT_46=1647,20
PRIMER_LEFT_46_TM=59.691
PRIMER_RIGHT_46_TM=59.711
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=838
PRIMER_PAIR_47_PENALTY=0.597675
PRIMER_LEFT_47_PENALTY=0.308871
PRIMER_RIGHT_47_PENALTY=0.288804
PRIMER_LEFT_47_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_47_SEQUENCE=AAAAGTTCCCAGCTCCAACA
PRIMER_LEFT_47=709,20
PRIMER_RIGHT_47=1644,20
PRIMER_LEFT_47_TM=59.691
PRIMER_RIGHT_47_TM=59.711
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=936
PRIMER_PAIR_48_PENALTY=0.597675
PRIMER_LEFT_48_PENALTY=0.308871
PRIMER_RIGHT_48_PENALTY=0.288804
PRIMER_LEFT_48_SEQUENCE=CTGGGACAAGGAAGGTGAAG
PRIMER_RIGHT_48_SEQUENCE=ACAAAAAGTTCCCAGCTCCA
PRIMER_LEFT_48=709,20
PRIMER_RIGHT_48=1647,20
PRIMER_LEFT_48_TM=59.691
PRIMER_RIGHT_48_TM=59.711
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=939
PRIMER_PAIR_49_PENALTY=0.600375
PRIMER_LEFT_49_PENALTY=0.571122
PRIMER_RIGHT_49_PENALTY=0.029253
PRIMER_LEFT_49_SEQUENCE=CCGCATATAGGAACCCAAAG
PRIMER_RIGHT_49_SEQUENCE=CCTCCACATGGTTTTTGCTT
PRIMER_LEFT_49=832,20
PRIMER_RIGHT_49=1317,20
PRIMER_LEFT_49_TM=59.429
PRIMER_RIGHT_49_TM=59.971
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=486
=
SEQUENCE_ID=MAGIC.329_3_12812758_12812898_no-0_mt-0_40664_344158_TRUE
SEQUENCE_TARGET=950,240
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_7290612_7290701_no-0_oy-0_191472_157298_TRUE
SEQUENCE_TARGET=950,189
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_704080_704237_kn-0_hi-0_24195_148586_TRUE
SEQUENCE_TARGET=950,257
SEQUENCE_TEMPLATE=AAACAATAAAGATGTGTGATTTATCTTACCAGTCCAATCCCCATGCCATACGTAACTCGTCCAATTATCAGTATGGAAAAGACAGGTGCTACTACAGTCACAATGGCTCCAACNAGATACAAGAATGCAGCCAAAATCAGCTCCTTTCTCCTTCCTTGAAAATTTAGTCCAACAATTCAATTTCTTTCAGATGAAGGAGACTGCTGCTACATACTCAGATGAAGACAGTTGAGAAAAACCAACCTATAATGTCGGCAACACTAAATGCAACAATGGAGCCAATTAAGGCACCATACAGTGAGCCACTGGTCTGCACAAAANTATGAAAATTGTCGTAAGCAAATTTNTTTGTCCTTCCACAAGAAAGATCACAGCTTCCCAATTTAGGGAGAAAATAAGAACAAAAAATAGTGCAATAACCAACATGTAGAAGATCCAAGAAAGGTANNCTCACAATTATACCNACATCCACTGAAGACAAGTCGTACCATGAAATTCCACTTAGAGTAGGCGACNGAGGCAAAAGCAGATTANTCATTCAGAATTTAANAGGCTTAGACAATCGAACANATGTACATGCATAANAACTATGCAGGAGAAATCCANGGAAACGNAATGGCAGTGCAAATGCAGGCATGAACTATGAAGTAGCGTCATTTAACTGCTAAAGANATGAGAAAGCTAAAAAAATCAAACTTGAGTATAGAAGCACAGTTTTAGACAAGGTTAAACNATTACCTTAAGAGACATGATAGCACAAGATGTTGCACCAATTTCATAACCAAAAAGCAANGCTCCAAGAGCTGGAAAGAGAAANCTGCAATAAATGACTTGTNACGTTCTTACGTTCTTAGCATGCTTTTAGAAAAATCAGTATCATGTGCATATCCAAAAGATTCCCACTTGCTTTCAGCAGAAATAGTAGTAACAAACAATAAATAGCAAATAAACAAAACNATCTGGGACCACAAATTGATGANTAATAAACAAGNGANAACGNNAGAGACTCACGGAGGAATGGCTGCAAGAACAGAGTAGTTCTCTGGGCTGTGGTTTTCCTTTAACAAAGGTTCCTTTTCAGCAGTAATCCCACCTGAAGAAGAATCACCAACAACCTACATAATATATNGTTTCAGGAATTTTGCATCAGAAAGTGANTTGATACCAAATTAGAGAGATTTAGGATAGAATCGTTATTCCAAAACCCAGAAGCTAAGGTCTTAAAATTGAAATCCCAATTTTCACAAGCTCCACACATTACAGAGACATCAAAATCCACAATCACTTCATAAAAAGGATATCTAAAATCAAGCCAAACCCTAAGATATACATTGCAATCAAAAGTCTTCAAAGCCGACCGGGAGAAGAACCAACAAAAATCAGTACAATTTGCTATCGGAAACCTAAACCGAAAGAAGAATGGGGAAAAGAAGACGATAATGAGAAAAGCGAAACCTGTCCAACGGAAGAGATCGATTGGTTCTCGGGATCAAACCCCATGGCTACGGAAGCTTGGTATGGATCCCGTGACNGTGAGTGTGGTATGTTCAAGGAAACCCAGAAAANAATATATCGTTCTGGGGTTGTACGAAATAGCTACTTCTCTGATTCGAGCTCAACGGAGCTTCGAATTTGCAGAGGAGAGAAACGTGAGGAAGGGAGACGACAAGTATGTATGCCAGGGTGATTATAGATGTTAACGGAACAGTGGACAAGCTTTAAATAGTCGGAGCTTAATTTCAAATTAAGATTCTGATTTTCAATTAAAAAAATAGGAAATTAGTCTTAATAGTGTGTTAGCATAAATAGAGAGAGTCAAACTGTGAAACCCTTTTTGTTTTTAAATTGTAAAACCAATTAAATGTTGATATTGGATGTTTCTGTTAATCAAAATTCCAGTTTCGTTTTTTTTTNTTTAGAAAGATTATAANNTTTTTTTNTAACAAAGTTGACCATAAAAACGACAGAAGAAGGATAATTAAAAAACGTGGTGCTGATCTCGTTNNAATTTTTAATTACCATTAAAAACGTTTTGTTGCTNGCACTGCGTTCGATTTATCTGNTTATCATAATCCGTTTTGGGAAAAATCTCTCATTTAGATGTNTTAATAGTATTAAGATTTTGTTGGAAGANTTTTTTTTTGTTTTTTGTTTTTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.742908
PRIMER_LEFT_0_PENALTY=0.691563
PRIMER_RIGHT_0_PENALTY=0.051345
PRIMER_LEFT_0_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_0_SEQUENCE=GGAGCTTGTGAAAATTGGGA
PRIMER_LEFT_0=793,20
PRIMER_RIGHT_0=1251,20
PRIMER_LEFT_0_TM=59.308
PRIMER_RIGHT_0_TM=60.051
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=459
PRIMER_PAIR_1_PENALTY=0.749090
PRIMER_LEFT_1_PENALTY=0.697745
PRIMER_RIGHT_1_PENALTY=0.051345
PRIMER_LEFT_1_SEQUENCE=CGGCAACACTAAATGCAACA
PRIMER_RIGHT_1_SEQUENCE=GGAGCTTGTGAAAATTGGGA
PRIMER_LEFT_1=252,20
PRIMER_RIGHT_1=1251,20
PRIMER_LEFT_1_TM=60.698
PRIMER_RIGHT_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=1000
PRIMER_PAIR_2_PENALTY=0.838858
PRIMER_LEFT_2_PENALTY=0.691563
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_2_SEQUENCE=TGGACAGGTTTCGCTTTTCT
PRIMER_LEFT_2=793,20
PRIMER_RIGHT_2=1461,20
PRIMER_LEFT_2_TM=59.308
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=669
PRIMER_PAIR_3_PENALTY=0.922835
PRIMER_LEFT_3_PENALTY=0.691563
PRIMER_RIGHT_3_PENALTY=0.231272
PRIMER_LEFT_3_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_3_SEQUENCE=AGCTTGTCCACTGTTCCGTT
PRIMER_LEFT_3=793,20
PRIMER_RIGHT_3=1717,20
PRIMER_LEFT_3_TM=59.308
PRIMER_RIGHT_3_TM=59.769
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=925
PRIMER_PAIR_4_PENALTY=0.929179
PRIMER_LEFT_4_PENALTY=0.691563
PRIMER_RIGHT_4_PENALTY=0.237616
PRIMER_LEFT_4_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_4_SEQUENCE=TTGTCGTCTCCCTTCCTCAC
PRIMER_LEFT_4=793,20
PRIMER_RIGHT_4=1668,20
PRIMER_LEFT_4_TM=59.308
PRIMER_RIGHT_4_TM=60.238
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=876
PRIMER_PAIR_5_PENALTY=0.976856
PRIMER_LEFT_5_PENALTY=0.829561
PRIMER_RIGHT_5_PENALTY=0.147295
PRIMER_LEFT_5_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_5_SEQUENCE=TGGACAGGTTTCGCTTTTCT
PRIMER_LEFT_5=888,20
PRIMER_RIGHT_5=1461,20
PRIMER_LEFT_5_TM=59.170
PRIMER_RIGHT_5_TM=59.853
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=574
PRIMER_PAIR_6_PENALTY=0.982896
PRIMER_LEFT_6_PENALTY=0.691563
PRIMER_RIGHT_6_PENALTY=0.291333
PRIMER_LEFT_6_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_6_SEQUENCE=CCCCATTCTTCTTTCGGTTT
PRIMER_LEFT_6=793,20
PRIMER_RIGHT_6=1424,20
PRIMER_LEFT_6_TM=59.308
PRIMER_RIGHT_6_TM=60.291
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=632
PRIMER_PAIR_7_PENALTY=0.994385
PRIMER_LEFT_7_PENALTY=0.691563
PRIMER_RIGHT_7_PENALTY=0.302821
PRIMER_LEFT_7_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_7_SEQUENCE=TCTCTTCCGTTGGACAGGTT
PRIMER_LEFT_7=793,20
PRIMER_RIGHT_7=1471,20
PRIMER_LEFT_7_TM=59.308
PRIMER_RIGHT_7_TM=59.697
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=679
PRIMER_PAIR_8_PENALTY=1.004903
PRIMER_LEFT_8_PENALTY=0.953558
PRIMER_RIGHT_8_PENALTY=0.051345
PRIMER_LEFT_8_SEQUENCE=AATGGAGCCAATTAAGGCAC
PRIMER_RIGHT_8_SEQUENCE=GGAGCTTGTGAAAATTGGGA
PRIMER_LEFT_8=271,20
PRIMER_RIGHT_8=1251,20
PRIMER_LEFT_8_TM=59.046
PRIMER_RIGHT_8_TM=60.051
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=981
PRIMER_PAIR_9_PENALTY=1.011932
PRIMER_LEFT_9_PENALTY=0.960587
PRIMER_RIGHT_9_PENALTY=0.051345
PRIMER_LEFT_9_SEQUENCE=CAATGGAGCCAATTAAGGCA
PRIMER_RIGHT_9_SEQUENCE=GGAGCTTGTGAAAATTGGGA
PRIMER_LEFT_9=270,20
PRIMER_RIGHT_9=1251,20
PRIMER_LEFT_9_TM=60.961
PRIMER_RIGHT_9_TM=60.051
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=982
PRIMER_PAIR_10_PENALTY=1.036777
PRIMER_LEFT_10_PENALTY=0.691563
PRIMER_RIGHT_10_PENALTY=0.345214
PRIMER_LEFT_10_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_10_SEQUENCE=GATCTCTTCCGTTGGACAGG
PRIMER_LEFT_10=793,20
PRIMER_RIGHT_10=1473,20
PRIMER_LEFT_10_TM=59.308
PRIMER_RIGHT_10_TM=59.655
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=681
PRIMER_PAIR_11_PENALTY=1.056917
PRIMER_LEFT_11_PENALTY=0.829561
PRIMER_RIGHT_11_PENALTY=0.227356
PRIMER_LEFT_11_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_11_SEQUENCE=TTGGACAGGTTTCGCTTTTC
PRIMER_LEFT_11=888,20
PRIMER_RIGHT_11=1462,20
PRIMER_LEFT_11_TM=59.170
PRIMER_RIGHT_11_TM=60.227
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=575
PRIMER_PAIR_12_PENALTY=1.060833
PRIMER_LEFT_12_PENALTY=0.829561
PRIMER_RIGHT_12_PENALTY=0.231272
PRIMER_LEFT_12_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_12_SEQUENCE=AGCTTGTCCACTGTTCCGTT
PRIMER_LEFT_12=888,20
PRIMER_RIGHT_12=1717,20
PRIMER_LEFT_12_TM=59.170
PRIMER_RIGHT_12_TM=59.769
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=9.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=830
PRIMER_PAIR_13_PENALTY=1.067177
PRIMER_LEFT_13_PENALTY=0.829561
PRIMER_RIGHT_13_PENALTY=0.237616
PRIMER_LEFT_13_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_13_SEQUENCE=TTGTCGTCTCCCTTCCTCAC
PRIMER_LEFT_13=888,20
PRIMER_RIGHT_13=1668,20
PRIMER_LEFT_13_TM=59.170
PRIMER_RIGHT_13_TM=60.238
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=781
PRIMER_PAIR_14_PENALTY=1.068312
PRIMER_LEFT_14_PENALTY=0.691563
PRIMER_RIGHT_14_PENALTY=0.376749
PRIMER_LEFT_14_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_14_SEQUENCE=CTTGTCGTCTCCCTTCCTCA
PRIMER_LEFT_14=793,20
PRIMER_RIGHT_14=1669,20
PRIMER_LEFT_14_TM=59.308
PRIMER_RIGHT_14_TM=60.377
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=877
PRIMER_PAIR_15_PENALTY=1.120894
PRIMER_LEFT_15_PENALTY=0.829561
PRIMER_RIGHT_15_PENALTY=0.291333
PRIMER_LEFT_15_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_15_SEQUENCE=CCCCATTCTTCTTTCGGTTT
PRIMER_LEFT_15=888,20
PRIMER_RIGHT_15=1424,20
PRIMER_LEFT_15_TM=59.170
PRIMER_RIGHT_15_TM=60.291
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=537
PRIMER_PAIR_16_PENALTY=1.132382
PRIMER_LEFT_16_PENALTY=0.829561
PRIMER_RIGHT_16_PENALTY=0.302821
PRIMER_LEFT_16_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_16_SEQUENCE=TCTCTTCCGTTGGACAGGTT
PRIMER_LEFT_16=888,20
PRIMER_RIGHT_16=1471,20
PRIMER_LEFT_16_TM=59.170
PRIMER_RIGHT_16_TM=59.697
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=584
PRIMER_PAIR_17_PENALTY=1.174775
PRIMER_LEFT_17_PENALTY=0.829561
PRIMER_RIGHT_17_PENALTY=0.345214
PRIMER_LEFT_17_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_17_SEQUENCE=GATCTCTTCCGTTGGACAGG
PRIMER_LEFT_17=888,20
PRIMER_RIGHT_17=1473,20
PRIMER_LEFT_17_TM=59.170
PRIMER_RIGHT_17_TM=59.655
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=586
PRIMER_PAIR_18_PENALTY=1.206310
PRIMER_LEFT_18_PENALTY=0.829561
PRIMER_RIGHT_18_PENALTY=0.376749
PRIMER_LEFT_18_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_18_SEQUENCE=CTTGTCGTCTCCCTTCCTCA
PRIMER_LEFT_18=888,20
PRIMER_RIGHT_18=1669,20
PRIMER_LEFT_18_TM=59.170
PRIMER_RIGHT_18_TM=60.377
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=782
PRIMER_PAIR_19_PENALTY=1.285149
PRIMER_LEFT_19_PENALTY=0.691563
PRIMER_RIGHT_19_PENALTY=0.593585
PRIMER_LEFT_19_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_19_SEQUENCE=CAATCGATCTCTTCCGTTGG
PRIMER_LEFT_19=793,20
PRIMER_RIGHT_19=1478,20
PRIMER_LEFT_19_TM=59.308
PRIMER_RIGHT_19_TM=60.594
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=686
PRIMER_PAIR_20_PENALTY=1.285149
PRIMER_LEFT_20_PENALTY=0.691563
PRIMER_RIGHT_20_PENALTY=0.593585
PRIMER_LEFT_20_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_20_SEQUENCE=CCAATCGATCTCTTCCGTTG
PRIMER_LEFT_20=793,20
PRIMER_RIGHT_20=1479,20
PRIMER_LEFT_20_TM=59.308
PRIMER_RIGHT_20_TM=60.594
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=8.7000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=687
PRIMER_PAIR_21_PENALTY=1.423147
PRIMER_LEFT_21_PENALTY=0.829561
PRIMER_RIGHT_21_PENALTY=0.593585
PRIMER_LEFT_21_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_21_SEQUENCE=CAATCGATCTCTTCCGTTGG
PRIMER_LEFT_21=888,20
PRIMER_RIGHT_21=1478,20
PRIMER_LEFT_21_TM=59.170
PRIMER_RIGHT_21_TM=60.594
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=591
PRIMER_PAIR_22_PENALTY=1.423147
PRIMER_LEFT_22_PENALTY=0.829561
PRIMER_RIGHT_22_PENALTY=0.593585
PRIMER_LEFT_22_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_22_SEQUENCE=CCAATCGATCTCTTCCGTTG
PRIMER_LEFT_22=888,20
PRIMER_RIGHT_22=1479,20
PRIMER_LEFT_22_TM=59.170
PRIMER_RIGHT_22_TM=60.594
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=8.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=592
PRIMER_PAIR_23_PENALTY=1.473072
PRIMER_LEFT_23_PENALTY=0.691563
PRIMER_RIGHT_23_PENALTY=0.781508
PRIMER_LEFT_23_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_23_SEQUENCE=GTTGGACAGGTTTCGCTTTT
PRIMER_LEFT_23=793,20
PRIMER_RIGHT_23=1463,20
PRIMER_LEFT_23_TM=59.308
PRIMER_RIGHT_23_TM=59.218
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=671
PRIMER_PAIR_24_PENALTY=1.488384
PRIMER_LEFT_24_PENALTY=0.691563
PRIMER_RIGHT_24_PENALTY=0.796820
PRIMER_LEFT_24_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_24_SEQUENCE=TCCCCATTCTTCTTTCGGTT
PRIMER_LEFT_24=793,20
PRIMER_RIGHT_24=1425,20
PRIMER_LEFT_24_TM=59.308
PRIMER_RIGHT_24_TM=60.797
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=9.9000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=633
PRIMER_PAIR_25_PENALTY=1.488384
PRIMER_LEFT_25_PENALTY=0.691563
PRIMER_RIGHT_25_PENALTY=0.796820
PRIMER_LEFT_25_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_25_SEQUENCE=TTCCCCATTCTTCTTTCGGT
PRIMER_LEFT_25=793,20
PRIMER_RIGHT_25=1426,20
PRIMER_LEFT_25_TM=59.308
PRIMER_RIGHT_25_TM=60.797
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=634
PRIMER_PAIR_26_PENALTY=1.508499
PRIMER_LEFT_26_PENALTY=0.691563
PRIMER_RIGHT_26_PENALTY=0.816936
PRIMER_LEFT_26_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_26_SEQUENCE=CTCTTCCGTTGGACAGGTTT
PRIMER_LEFT_26=793,20
PRIMER_RIGHT_26=1470,20
PRIMER_LEFT_26_TM=59.308
PRIMER_RIGHT_26_TM=59.183
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=678
PRIMER_PAIR_27_PENALTY=1.563827
PRIMER_LEFT_27_PENALTY=1.512483
PRIMER_RIGHT_27_PENALTY=0.051345
PRIMER_LEFT_27_SEQUENCE=GCCAATTAAGGCACCATACAG
PRIMER_RIGHT_27_SEQUENCE=GGAGCTTGTGAAAATTGGGA
PRIMER_LEFT_27=277,21
PRIMER_RIGHT_27=1251,20
PRIMER_LEFT_27_TM=59.488
PRIMER_RIGHT_27_TM=60.051
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.7000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=975
PRIMER_PAIR_28_PENALTY=1.590597
PRIMER_LEFT_28_PENALTY=0.691563
PRIMER_RIGHT_28_PENALTY=0.899034
PRIMER_LEFT_28_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_28_SEQUENCE=TCGTCTTCTTTTCCCCATTC
PRIMER_LEFT_28=793,20
PRIMER_RIGHT_28=1436,20
PRIMER_LEFT_28_TM=59.308
PRIMER_RIGHT_28_TM=59.101
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=644
PRIMER_PAIR_29_PENALTY=1.590963
PRIMER_LEFT_29_PENALTY=1.539618
PRIMER_RIGHT_29_PENALTY=0.051345
PRIMER_LEFT_29_SEQUENCE=CAACAATGGAGCCAATTAAGG
PRIMER_RIGHT_29_SEQUENCE=GGAGCTTGTGAAAATTGGGA
PRIMER_LEFT_29=267,21
PRIMER_RIGHT_29=1251,20
PRIMER_LEFT_29_TM=59.460
PRIMER_RIGHT_29_TM=60.051
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.5000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=985
PRIMER_PAIR_30_PENALTY=1.597821
PRIMER_LEFT_30_PENALTY=0.691563
PRIMER_RIGHT_30_PENALTY=0.906258
PRIMER_LEFT_30_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_30_SEQUENCE=ATCGATCTCTTCCGTTGGAC
PRIMER_LEFT_30=793,20
PRIMER_RIGHT_30=1476,20
PRIMER_LEFT_30_TM=59.308
PRIMER_RIGHT_30_TM=59.094
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=684
PRIMER_PAIR_31_PENALTY=1.611070
PRIMER_LEFT_31_PENALTY=0.829561
PRIMER_RIGHT_31_PENALTY=0.781508
PRIMER_LEFT_31_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_31_SEQUENCE=GTTGGACAGGTTTCGCTTTT
PRIMER_LEFT_31=888,20
PRIMER_RIGHT_31=1463,20
PRIMER_LEFT_31_TM=59.170
PRIMER_RIGHT_31_TM=59.218
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=576
PRIMER_PAIR_32_PENALTY=1.616005
PRIMER_LEFT_32_PENALTY=1.468710
PRIMER_RIGHT_32_PENALTY=0.147295
PRIMER_LEFT_32_SEQUENCE=CCAAAAGATTCCCACTTGCTT
PRIMER_RIGHT_32_SEQUENCE=TGGACAGGTTTCGCTTTTCT
PRIMER_LEFT_32=888,21
PRIMER_RIGHT_32=1461,20
PRIMER_LEFT_32_TM=60.469
PRIMER_RIGHT_32_TM=59.853
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=574
PRIMER_PAIR_33_PENALTY=1.626382
PRIMER_LEFT_33_PENALTY=0.829561
PRIMER_RIGHT_33_PENALTY=0.796820
PRIMER_LEFT_33_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_33_SEQUENCE=TCCCCATTCTTCTTTCGGTT
PRIMER_LEFT_33=888,20
PRIMER_RIGHT_33=1425,20
PRIMER_LEFT_33_TM=59.170
PRIMER_RIGHT_33_TM=60.797
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=9.9000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=538
PRIMER_PAIR_34_PENALTY=1.626382
PRIMER_LEFT_34_PENALTY=0.829561
PRIMER_RIGHT_34_PENALTY=0.796820
PRIMER_LEFT_34_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_34_SEQUENCE=TTCCCCATTCTTCTTTCGGT
PRIMER_LEFT_34=888,20
PRIMER_RIGHT_34=1426,20
PRIMER_LEFT_34_TM=59.170
PRIMER_RIGHT_34_TM=60.797
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=539
PRIMER_PAIR_35_PENALTY=1.646497
PRIMER_LEFT_35_PENALTY=0.829561
PRIMER_RIGHT_35_PENALTY=0.816936
PRIMER_LEFT_35_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_35_SEQUENCE=CTCTTCCGTTGGACAGGTTT
PRIMER_LEFT_35=888,20
PRIMER_RIGHT_35=1470,20
PRIMER_LEFT_35_TM=59.170
PRIMER_RIGHT_35_TM=59.183
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=583
PRIMER_PAIR_36_PENALTY=1.692364
PRIMER_LEFT_36_PENALTY=0.691563
PRIMER_RIGHT_36_PENALTY=1.000801
PRIMER_LEFT_36_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_36_SEQUENCE=TGGAGCTTGTGAAAATTGGG
PRIMER_LEFT_36=793,20
PRIMER_RIGHT_36=1252,20
PRIMER_LEFT_36_TM=59.308
PRIMER_RIGHT_36_TM=61.001
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=10.0000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=460
PRIMER_PAIR_37_PENALTY=1.696066
PRIMER_LEFT_37_PENALTY=1.468710
PRIMER_RIGHT_37_PENALTY=0.227356
PRIMER_LEFT_37_SEQUENCE=CCAAAAGATTCCCACTTGCTT
PRIMER_RIGHT_37_SEQUENCE=TTGGACAGGTTTCGCTTTTC
PRIMER_LEFT_37=888,21
PRIMER_RIGHT_37=1462,20
PRIMER_LEFT_37_TM=60.469
PRIMER_RIGHT_37_TM=60.227
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=575
PRIMER_PAIR_38_PENALTY=1.699982
PRIMER_LEFT_38_PENALTY=1.468710
PRIMER_RIGHT_38_PENALTY=0.231272
PRIMER_LEFT_38_SEQUENCE=CCAAAAGATTCCCACTTGCTT
PRIMER_RIGHT_38_SEQUENCE=AGCTTGTCCACTGTTCCGTT
PRIMER_LEFT_38=888,21
PRIMER_RIGHT_38=1717,20
PRIMER_LEFT_38_TM=60.469
PRIMER_RIGHT_38_TM=59.769
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=9.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=830
PRIMER_PAIR_39_PENALTY=1.704700
PRIMER_LEFT_39_PENALTY=0.691563
PRIMER_RIGHT_39_PENALTY=1.013137
PRIMER_LEFT_39_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_39_SEQUENCE=ATCTCTTCCGTTGGACAGGTT
PRIMER_LEFT_39=793,20
PRIMER_RIGHT_39=1472,21
PRIMER_LEFT_39_TM=59.308
PRIMER_RIGHT_39_TM=59.987
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=680
PRIMER_PAIR_40_PENALTY=1.706326
PRIMER_LEFT_40_PENALTY=1.468710
PRIMER_RIGHT_40_PENALTY=0.237616
PRIMER_LEFT_40_SEQUENCE=CCAAAAGATTCCCACTTGCTT
PRIMER_RIGHT_40_SEQUENCE=TTGTCGTCTCCCTTCCTCAC
PRIMER_LEFT_40=888,21
PRIMER_RIGHT_40=1668,20
PRIMER_LEFT_40_TM=60.469
PRIMER_RIGHT_40_TM=60.238
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=781
PRIMER_PAIR_41_PENALTY=1.728595
PRIMER_LEFT_41_PENALTY=0.829561
PRIMER_RIGHT_41_PENALTY=0.899034
PRIMER_LEFT_41_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_41_SEQUENCE=TCGTCTTCTTTTCCCCATTC
PRIMER_LEFT_41=888,20
PRIMER_RIGHT_41=1436,20
PRIMER_LEFT_41_TM=59.170
PRIMER_RIGHT_41_TM=59.101
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=549
PRIMER_PAIR_42_PENALTY=1.735819
PRIMER_LEFT_42_PENALTY=0.829561
PRIMER_RIGHT_42_PENALTY=0.906258
PRIMER_LEFT_42_SEQUENCE=CCAAAAGATTCCCACTTGCT
PRIMER_RIGHT_42_SEQUENCE=ATCGATCTCTTCCGTTGGAC
PRIMER_LEFT_42=888,20
PRIMER_RIGHT_42=1476,20
PRIMER_LEFT_42_TM=59.170
PRIMER_RIGHT_42_TM=59.094
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=589
PRIMER_PAIR_43_PENALTY=1.760043
PRIMER_LEFT_43_PENALTY=1.468710
PRIMER_RIGHT_43_PENALTY=0.291333
PRIMER_LEFT_43_SEQUENCE=CCAAAAGATTCCCACTTGCTT
PRIMER_RIGHT_43_SEQUENCE=CCCCATTCTTCTTTCGGTTT
PRIMER_LEFT_43=888,21
PRIMER_RIGHT_43=1424,20
PRIMER_LEFT_43_TM=60.469
PRIMER_RIGHT_43_TM=60.291
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=537
PRIMER_PAIR_44_PENALTY=1.771531
PRIMER_LEFT_44_PENALTY=1.468710
PRIMER_RIGHT_44_PENALTY=0.302821
PRIMER_LEFT_44_SEQUENCE=CCAAAAGATTCCCACTTGCTT
PRIMER_RIGHT_44_SEQUENCE=TCTCTTCCGTTGGACAGGTT
PRIMER_LEFT_44=888,21
PRIMER_RIGHT_44=1471,20
PRIMER_LEFT_44_TM=60.469
PRIMER_RIGHT_44_TM=59.697
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=584
PRIMER_PAIR_45_PENALTY=1.780032
PRIMER_LEFT_45_PENALTY=0.691563
PRIMER_RIGHT_45_PENALTY=1.088469
PRIMER_LEFT_45_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_45_SEQUENCE=GGACAGGTTTCGCTTTTCTC
PRIMER_LEFT_45=793,20
PRIMER_RIGHT_45=1460,20
PRIMER_LEFT_45_TM=59.308
PRIMER_RIGHT_45_TM=58.912
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=668
PRIMER_PAIR_46_PENALTY=1.813924
PRIMER_LEFT_46_PENALTY=1.468710
PRIMER_RIGHT_46_PENALTY=0.345214
PRIMER_LEFT_46_SEQUENCE=CCAAAAGATTCCCACTTGCTT
PRIMER_RIGHT_46_SEQUENCE=GATCTCTTCCGTTGGACAGG
PRIMER_LEFT_46=888,21
PRIMER_RIGHT_46=1473,20
PRIMER_LEFT_46_TM=60.469
PRIMER_RIGHT_46_TM=59.655
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=586
PRIMER_PAIR_47_PENALTY=1.825967
PRIMER_LEFT_47_PENALTY=0.691563
PRIMER_RIGHT_47_PENALTY=1.134403
PRIMER_LEFT_47_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_47_SEQUENCE=CCCTTCCTCACGTTTCTCTC
PRIMER_LEFT_47=793,20
PRIMER_RIGHT_47=1659,20
PRIMER_LEFT_47_TM=59.308
PRIMER_RIGHT_47_TM=58.866
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=867
PRIMER_PAIR_48_PENALTY=1.825967
PRIMER_LEFT_48_PENALTY=0.691563
PRIMER_RIGHT_48_PENALTY=1.134403
PRIMER_LEFT_48_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_48_SEQUENCE=CTCCCTTCCTCACGTTTCTC
PRIMER_LEFT_48=793,20
PRIMER_RIGHT_48=1661,20
PRIMER_LEFT_48_TM=59.308
PRIMER_RIGHT_48_TM=58.866
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=869
PRIMER_PAIR_49_PENALTY=1.825967
PRIMER_LEFT_49_PENALTY=0.691563
PRIMER_RIGHT_49_PENALTY=1.134403
PRIMER_LEFT_49_SEQUENCE=GCTCCAAGAGCTGGAAAGAG
PRIMER_RIGHT_49_SEQUENCE=TCTCCCTTCCTCACGTTTCT
PRIMER_LEFT_49=793,20
PRIMER_RIGHT_49=1662,20
PRIMER_LEFT_49_TM=59.308
PRIMER_RIGHT_49_TM=58.866
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=870
=
SEQUENCE_ID=MAGIC.426_3_14272351_14272972_zu-0_hi-0_233349_14637_TRUE
SEQUENCE_TARGET=950,721
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGATNNNAAAAAANATGTATATTAGGATCGTAATCAAATTTTATACNAATTAATTAGTATTAAAATAAAANNNNNNNNTAAAACNTGTGTNAAGTTGATCCTATCTACGNNACAATATAGTTTCTAGAAGTTATCTCTGNCCTTTNTCGCTTACAACCAACTTGATAGTGATTTTTGGATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4380257_4380363_ler-0_tsu-0_5310_65625_TRUE
SEQUENCE_TARGET=950,206
SEQUENCE_TEMPLATE=NAAAGCCTCTNAGGTAACNCAAAGNAAAAANGTTNAAAAGCTCCCTNNGNCAAACAACCNNTAAGTGTTAANACCCCTTGGNAAAATANAAAATTCCCTCTCGNGAAANTCGTGTTNATAAAANAAATTGGGACAATCCCCNANTNTTATGGTCATAGCCATNAAAGAGTTAANTTGNGCCGNAAGCCTCATCAACATTCGGTGTGATTTCGTCTTGGTNNNNNNTAGCAATGGTCGTTCCTTGCTCGCGAGTTTNNGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANACATTTTGGCGANTTCGAGATCAGCTTGCAGGAACTTANNNNNNNNNNNNNNNNGNCACNTTNACNAGNAAATTAGNCCTCCGACCAAGTNAANTTACTCGAANNACNGGNGGCTTATGTTGATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNCNATGTTGCACAAAGGAGGTGGTCAAATCTTGACATNAATTTGTNGTTNAANNNNNNNNNNNNNNNNNNNNGTCAAAAGAATAAACTTGTNNNNNNNNNNNNTGAATTGATTGTGGAAGTTATTTTGTGAATTAATNCTATAATTAATAGCCAAATAATGGCACATTGGAAGAAGGAAAGAGACTATAAAAGAGAGGTGAAAACTCCANNAAAANGGGCTAGAGATTCTACATTTTCACCACAAATCTTAATATTAAACATTGTTATTTTCTGATAAATACACAAAAGATTATTTCTTCCCTTTTTATGTAAGATCTTTATACAAGAAATATTATACCATTTTTTATGTACAATTTCTTCGTACATTTCTATATACTTACTTATACTTTTATTATGCACATTTGTCTTGTACTTTTGTACTCGAGANNTTTNGCCCACAAATAATNNNNATACTTGAGTGTNTGAATCCNACATNCANNNNNCNNNNCTCCGGTNGATCAATATGTNGCTCAAACTNGATTTGNTGCTCATAGTCAGCTTGAGTAAAGTACTCATGTTGCTCATNTTCAGCTTNANTATATTCCGNTTNATNNTCTCGAGNTCCTCCAATGTCGATGTTCAAGGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4110260_4110554_wu-0_oy-0_1293942_17673_TRUE
SEQUENCE_TARGET=950,394
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATNCTNNAGTGTGTGAATCCGTCTTCCACATNTTGGCGCCGTCTGTNGGGANCGGAGGATTCTTGAGTCGTCAGATTTCTTCNGTGTATTACATACCCNAAGTNAGNCGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_5166171_5166421_sf-2_tsu-0_13316_301527_TRUE
SEQUENCE_TARGET=950,350
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNCGNTNTAANNTTTCCATTTATTTATNTAGGAGGGTTTTATACGTGAGACCTTGGCTTAAATATAGNNTTTTNTTTTTGATTANTAAAAGAGNAATNTATTTTTGAACCGCAAANNTNTTNTNNNCTTAAAAGTACGAATTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGTGGGTTTNTTTTNAGATTTTATANTAGTGAAANCACACAATTAANAAATTCATTTTNCTTCTNTTTTGGTTTAATTTGGNTGGTTGCNGTTGTTTTGCACACCCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACCACACNNACATTTTCATGCCCAGTNAAAGCTTGTAGTGNNNNTCNACCTCTCTGTTTANATCTCNCACATCTATTNTTGATGTGCCTNCGTATGGGACCNCTCGCTTAGNTTCACCTTTGTCTCCTCCCATATATATCNCGGANCGAANNNNNNNNNNNNNNNAACCANTAAGACTCNGTGTTTNTANTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAGAACANCATGAAAGATCCCACAGNTCGGAATTAGGAGCCACGTGAGTCACNANCAGAGCTGTCCAAAACAAATCTAATTTCATATTTCAGATTCTCCATATTATTTAATCTGAAACGANAATCTAAGCTATGAAAGCCGAAAATGTACAATTTCANATGTCAAAGACAAATTANATTGACTTCAACCTAGGTTAGATATATATTAAGTGACNAANNAAATACTNGGNTGAGGCCTTTTAGGGATATTGAGGNNACCANAGTAGAAGAAACATNTTATTATGNGATNAGATNANTTTTTTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANAAAACTGATTATAAACTTTATTAGAGAGACCGCACGACAAGAATCCATACATNTAACATATAAGACTATATGGTGAAAAGAAACTAATNAAGAAGATTANAAAACAAAAANAAANAANAAAAGAGTACGTTAACAGAGAGGAGATCGTTGAGGAACGCCTTTTCGCCGATTGATGCAAGCAGAAATATCGTCAGGATCCTCCGAAACTTCTTTATAACTATTAGCCGATTTNGCGCAAAGGCAAAAACCAGTCCTTTTTTTCCTCACGACATGGGTGGTGGTTGCGGCATAAGCAGCNGCCGCACCCGCATTGTTGTTATCCGAGGAAGCNGAGGACCAGCTCATGGACTCATCNCCGTCTACCACANTTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_14036255_14036330_tsu-0_ler-0_22057_9221_TRUE
SEQUENCE_TARGET=950,175
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGTTCGACTNTNNGAACGAACAAGGNNAAGNAGTTCGGNTGTGNGAACNTACNANNANAAGAAACAAAANNANNTTTNTTNANNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGCGNGCNCTTCGAANTANGNAACTNTTNTTGTCTTGCAAAACACTTGNACTCCTTNGCCGGNGAGGTCAAGAATNGGCATCATANAGTTTGGTATGCGANCAAACTCGGAGATNNNNNNNNNNNTCCANTCCCNGNNANNNGAGNTAAGGGTNTGAAGCTNCCTTNNTATCACNGNGNNNGTNGNCGGAGAGGAACAGAGGGCTGCCGGGNTGAAGAAGAGAAACNGTGGAGGCTGCTTCGTTCTCCATTTGTGTANTGTTTATCAAGTNAATTTCTGAAATCANACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAGGGNTTTTCCTTGGAACCACAAGTTTAATAACTGTGGCGTGTCCGAATGGTTTAAACCATTTTTGGGAATCNAGAAAAANCAATTATNNCCTCAAAGGNGTGAAGCTTTTTCNTGTTTNTCGAGAAAGGANATGNCAANNGTCGCATNAAANACNAAAANCCCATTAAATNNNNNNNNNANAGTTNAGGCNCAAGCCNANNNAANAAGCNGAANTGATTAAACCNGAGTNGGGGANTTCGNNNNNNNAANCGAGCTCGGTTCTCGCNTTTTAAGAGAAAGGTNNNNNNNNNNNNAAAATCTNGAAACTTAGNNACANGGCNANAAGNTNTNTAAAGACGGACTNTNGNAANATNATAGNNNNAAAAGGTTTAAAAGCCTCCTCNGGCAANTACTTNTTNAAAAATNNAGCNTCNNNNNNNNNCANCNNATTTATCCAAATTTAATTTCTAGTTTTGGAAGTGTTTGGGANAGCCCCNAATTAAAGTAGATACAACCACTAGGAATCCGTAATCTTCTCGGGGTCAACGATAGCCNGATCGGCTTCGGTTGGNTCAACAACAGCTGACTCAGTTTTGGTTGGTTCACNGGCAGAGAGTAGCTTAGAAGAGTTTCCCGGCTCAGTTCCGGCTTCATGTTCAACATCCGGGATGATCACTGTCTCACTNTTCACGANCGGACAGTAGTATCGACCANTTCACTAGAGCCTGAAGNGAACGTTTCAAGTCAGCAANAAACACAAATACAACAACTNNNGNNNATAANGNTN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14458324_14458335_tsu-0_wil-2_19391_11119_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGCTTGCTTAATTAGTTTTTGTTTATTAATTTCATGCTGAATAAGTTTTTCTAGATTGCATGAAATAGAAAAACAAGTNTNNATTGTGATTTAATAATCATATCAATATTCAGGTTTTATTGTAGTCGNACTANGCCTAAGTGTATGCTGGTCCGGGGAGCTATTACCATAGTTATTATAGCCATAAAACCTTCCACTCGTCTAATATTTAATAAGAAAGCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_11923150_11923571_no-0_oy-0_153502_296563_TRUE
SEQUENCE_TARGET=950,521
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4718918_4718923_edi-0_can-0_3160_10825_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACAATCNTTGTNNAAGGTCCCGCCTGTGAGTNGGCCCTGGATGTTCCTGCCCNTTTNACGNTTTTNNTTTCTTGAGNNGCTTGCTTCAGTTGGAGACTNGTATGG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_6483013_6483191_wu-0_ler-0_637805_166854_TRUE
SEQUENCE_TARGET=950,278
SEQUENCE_TEMPLATE=AAAAATNAATTTCANTANNTTATNGAAAANANACAGTCATCTGAAACGTNAATGCATGCATTCGATGACNACNNNNNNNNNNATTAGGTTTGGAANTGGAGANTANGTTTAATNGATCTNTTATCANTTTGTTTTGGTGAGATTNGATATAGNAACAGGNGGGAGGCGATCACTTTCNCTTTTTTCNGNAAGAAAAANCCNNATNTGCTCGNAAGGNAANGAGANNATTTGGTGACTNTACTNAANATGTATCATCATANATCTNTTGANTGAGATAAGATTTTGAGCGAGAGNTGGGTTNNAGATATGNATGTTNTCNATTGANGNTTTTNNNNNTTTGTTTGTTGATTNTGTCATGAANNTNTCACCCAATATANANAGNGATTTTTGGNAAANAAAACTTTTTATGNNCCTTNTNNNTTTTTTAGTAAAATNAATCNATTTTNNAAGTTTTTANTTNTTTTANTTTTNATGNGCCTNGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNTGCNTAGCCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATNTCCGTAGATAAAACATGTNACCAGATTTCTTACTTAANANNNACAAAGNNTAAAACTCGTGCGGTGTCCTTGTCCTTATATAACACATCTNTGAGACTATGACTCATGNGATATTGTACCATAANATNTCTCTCTCTTTCTCTCNNNATCGCTNTCACTTCAGATTTNANACCAATCATGGCAGCATCAAANCTCCTCGTATCCGACATNGCCTCTGTCGTTGATCACGTCCCTTCAAACTACGTNNGACCAGTCTCTGACCGTCCAAANATNTCNGANGTTNANANNTCCGGCGACTCTATTCCTCTAATCGATCTCCANGACCTCCATGGACCTAATCGANCNGACATTATCAATCAGTTTGCTCATGCATGCTCTTCTTNTGGCTTCTTCCAGGTAAGTGCACAAACAATCCACTTTNANTCTCCTATACTCTNGTTTCTTGAGTANCACTCNTATCTTTTCTTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_10562715_10562842_rsch-4_po-0_37715_17533_TRUE
SEQUENCE_TARGET=950,227
SEQUENCE_TEMPLATE=ATTNAAAATGTTTGTCCATTCAAATAAATAACCTTTACTAAATTATTTATTTGGTAGATTACAACCAAATATTGAACCAAAATTANAATAATTCTGTAAATAAGTGGTGCACTAAATAAGTAAATAATAAATGTCAAACCGGTTNTGANANNNNATAATAAAGAGAGATCATCAAANATNTCAAGAAAATNCNNNNTGATGATGGTTNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANATATGAACATCTTNTGTAACCTTNGAAAANATTTTGCATNAATTNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATTCTCAGTGGTTTACACANGAAGCAAGNNNNNNNNNNNNCTGACTAGNTTGTTAAGACTCTATNNTAAANTATATACAAATGCANGGAATGGAAATGACACGCTTCTTCATTGCAATTTTCTCGAAACTATATTGGAGGTAGCTTGTCTTTTCCTTCAAAGTAGCCNGAGACCCCGGCNTTCANCTGCAAAACGTTTGACATTTTNAGGACGATTNAAANGAAGAATAANTGGGTNNNNNNNNNNNNNNNTTAAAGAAGTTATAATACCTTTTCGATTCTGTCNGCTGTGCCTGAGAGATCTTGTGTTTGTTTTTTGAGCANGNTGATCCGGGCTTCCTTCTGGCGATTCTGTTCTTCAGCCTGGNNNNNNNNNNNNNACGAGAAGAAATTNTTTNTAAGAAATGGCAACATAAGCAAATGANNAGTTTCTTNTGCCTTCTTTTTGAGNTTACTTACCCTTTGGAGCAAAANTGTTAACTTTTGNATCTCGTTCNTCTTNGNTGTTGTAAGATCATNTGCNATNGCCATTACATCAGTCCTGAGATGGAGTCATGGTGACAGTAATATCAGAANTCCAAAATGTGGGGACTTGACTTACAATTGAGGCCAATACCATAAANGGANNATNGTAAACAAAATGGATTAACCAATATGCATGAGACATACTTATCNGAAAACAAGGNATCCAAACTCTGTTCTTCGACCAGCTCCTCNACTGTATCAAGAATTGGTCCGACCTGCAANNNGNAAANNNNNTCNGTCANAAGNNTNNTGANANAANCTCTNNCTTCACNAGTNTGTNGNTNNNAAANCAANTAAGATGTACCTGAGTTTCGTGACATTGTTCATCAAACTCTTCCTGTAACAATGATGAAAACATAACTAAGAGTTTNNNATTCATCACACNATGAATAGCCAAAAAATCACAAAAATTCATGTTTTANNATAAAAACAACAACTTGAGAAAANTTCAGAGCAAAGGGTTACTACCTCTATTGTCTGATGCAAATTCACAACAACCTAGAAACATGTCAGANAAAGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCAATGGATCGNAAGAGAATNATAAAGAGAGATACATACCCGAGTATACAACTGAAAAAGCAGTTCTTGCTCAGCCCCACTGAATTTCGAGAAAATNTCAACAAAATCCTACATAGTGATGGTTCACATAAAAANGGTTACTATTAGTTAGTTAGTACACNACACATAAACCNGAAACAAAATCAACAANACACTGNTANAGATAGATAGACAGTTCTCAAACCAGAAACNAAATCAAAAACCAAATTCAATTCTAATTAACTCAGGATCCAATCATCGAGAACACTTAAACCNTAAAACTTCAATTTTACCTAAATCAGACAGAACAAAACCCAAATTGCGNTTNAAAAAAANAAANNAACAATGGAAAATGATTATGACATTGAAGCATANACCTGTTTGGAGCAAGCAGTGAGAAGATGTCGAAGAGTGGATTTGAATGATTTCTTCAGATGGGTTCGTCTTGAACCCGNAATATCCGTTTCATGACCNGGTTTCTCCATCTTAACCCGAATGATAACNTTTTTTTGTTGGTTTAAAGATTCCGGAAGAAGAAGTTGCTAACTGGTTAAAAACAAATGTACAAGTTGNCAGAGANATCAAGGACGGNAAANAGAAAATATCCCGGGAAGGCCCAGTTATATAGGCCCANTTAAAACCCATAAGAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.230723
PRIMER_LEFT_0_PENALTY=0.186600
PRIMER_RIGHT_0_PENALTY=0.044122
PRIMER_LEFT_0_SEQUENCE=TTCCTTCTGGCGATTCTGTT
PRIMER_RIGHT_0_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_0=706,20
PRIMER_RIGHT_0=1552,20
PRIMER_LEFT_0_TM=59.813
PRIMER_RIGHT_0_TM=60.044
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=11.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=847
PRIMER_PAIR_1_PENALTY=0.383743
PRIMER_LEFT_1_PENALTY=0.339620
PRIMER_RIGHT_1_PENALTY=0.044122
PRIMER_LEFT_1_SEQUENCE=TCCTTCTGGCGATTCTGTTC
PRIMER_RIGHT_1_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_1=707,20
PRIMER_RIGHT_1=1552,20
PRIMER_LEFT_1_TM=60.340
PRIMER_RIGHT_1_TM=60.044
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=846
PRIMER_PAIR_2_PENALTY=0.522619
PRIMER_LEFT_2_PENALTY=0.478496
PRIMER_RIGHT_2_PENALTY=0.044122
PRIMER_LEFT_2_SEQUENCE=TCTGGCGATTCTGTTCTTCA
PRIMER_RIGHT_2_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_2=711,20
PRIMER_RIGHT_2=1552,20
PRIMER_LEFT_2_TM=59.522
PRIMER_RIGHT_2_TM=60.044
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=842
PRIMER_PAIR_3_PENALTY=0.614622
PRIMER_LEFT_3_PENALTY=0.570500
PRIMER_RIGHT_3_PENALTY=0.044122
PRIMER_LEFT_3_SEQUENCE=CCTTCTGGCGATTCTGTTCT
PRIMER_RIGHT_3_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_3=708,20
PRIMER_RIGHT_3=1552,20
PRIMER_LEFT_3_TM=59.430
PRIMER_RIGHT_3_TM=60.044
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=845
PRIMER_PAIR_4_PENALTY=0.614622
PRIMER_LEFT_4_PENALTY=0.570500
PRIMER_RIGHT_4_PENALTY=0.044122
PRIMER_LEFT_4_SEQUENCE=CTTCCTTCTGGCGATTCTGT
PRIMER_RIGHT_4_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_4=705,20
PRIMER_RIGHT_4=1552,20
PRIMER_LEFT_4_TM=59.430
PRIMER_RIGHT_4_TM=60.044
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=848
PRIMER_PAIR_5_PENALTY=0.979147
PRIMER_LEFT_5_PENALTY=0.954019
PRIMER_RIGHT_5_PENALTY=0.025128
PRIMER_LEFT_5_SEQUENCE=CCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_5_SEQUENCE=CGCAATTTGGGTTTTGTTCT
PRIMER_LEFT_5=910,20
PRIMER_RIGHT_5=1800,20
PRIMER_LEFT_5_TM=59.046
PRIMER_RIGHT_5_TM=59.975
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=891
PRIMER_PAIR_6_PENALTY=0.998141
PRIMER_LEFT_6_PENALTY=0.954019
PRIMER_RIGHT_6_PENALTY=0.044122
PRIMER_LEFT_6_SEQUENCE=CCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_6_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_6=910,20
PRIMER_RIGHT_6=1552,20
PRIMER_LEFT_6_TM=59.046
PRIMER_RIGHT_6_TM=60.044
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=11.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=643
PRIMER_PAIR_7_PENALTY=1.238393
PRIMER_LEFT_7_PENALTY=0.954019
PRIMER_RIGHT_7_PENALTY=0.284374
PRIMER_LEFT_7_SEQUENCE=CCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_7_SEQUENCE=TTCTCACTGCTTGCTCCAAA
PRIMER_LEFT_7=910,20
PRIMER_RIGHT_7=1876,20
PRIMER_LEFT_7_TM=59.046
PRIMER_RIGHT_7_TM=59.716
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=967
PRIMER_PAIR_8_PENALTY=1.792190
PRIMER_LEFT_8_PENALTY=1.748068
PRIMER_RIGHT_8_PENALTY=0.044122
PRIMER_LEFT_8_SEQUENCE=CCTTCTGGCGATTCTGTTCTT
PRIMER_RIGHT_8_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_8=708,21
PRIMER_RIGHT_8=1552,20
PRIMER_LEFT_8_TM=60.748
PRIMER_RIGHT_8_TM=60.044
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=11.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=845
PRIMER_PAIR_9_PENALTY=1.792190
PRIMER_LEFT_9_PENALTY=1.748068
PRIMER_RIGHT_9_PENALTY=0.044122
PRIMER_LEFT_9_SEQUENCE=CTTCCTTCTGGCGATTCTGTT
PRIMER_RIGHT_9_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_9=705,21
PRIMER_RIGHT_9=1552,20
PRIMER_LEFT_9_TM=60.748
PRIMER_RIGHT_9_TM=60.044
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=11.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=848
PRIMER_PAIR_10_PENALTY=1.942927
PRIMER_LEFT_10_PENALTY=1.898804
PRIMER_RIGHT_10_PENALTY=0.044122
PRIMER_LEFT_10_SEQUENCE=TTCTGGCGATTCTGTTCTTCA
PRIMER_RIGHT_10_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_10=710,21
PRIMER_RIGHT_10=1552,20
PRIMER_LEFT_10_TM=60.899
PRIMER_RIGHT_10_TM=60.044
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=11.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=843
PRIMER_PAIR_11_PENALTY=1.978000
PRIMER_LEFT_11_PENALTY=1.933877
PRIMER_RIGHT_11_PENALTY=0.044122
PRIMER_LEFT_11_SEQUENCE=CTTCTGGCGATTCTGTTCTTC
PRIMER_RIGHT_11_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_11=709,21
PRIMER_RIGHT_11=1552,20
PRIMER_LEFT_11_TM=59.066
PRIMER_RIGHT_11_TM=60.044
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=11.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=844
PRIMER_PAIR_12_PENALTY=2.024860
PRIMER_LEFT_12_PENALTY=1.980738
PRIMER_RIGHT_12_PENALTY=0.044122
PRIMER_LEFT_12_SEQUENCE=TTCTGGCGATTCTGTTCTTC
PRIMER_RIGHT_12_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_12=710,20
PRIMER_RIGHT_12=1552,20
PRIMER_LEFT_12_TM=58.019
PRIMER_RIGHT_12_TM=60.044
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=11.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=843
PRIMER_PAIR_13_PENALTY=2.027078
PRIMER_LEFT_13_PENALTY=2.001949
PRIMER_RIGHT_13_PENALTY=0.025128
PRIMER_LEFT_13_SEQUENCE=AGTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_13_SEQUENCE=CGCAATTTGGGTTTTGTTCT
PRIMER_LEFT_13=907,22
PRIMER_RIGHT_13=1800,20
PRIMER_LEFT_13_TM=59.998
PRIMER_RIGHT_13_TM=59.975
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.8000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=894
PRIMER_PAIR_14_PENALTY=2.034483
PRIMER_LEFT_14_PENALTY=2.009354
PRIMER_RIGHT_14_PENALTY=0.025128
PRIMER_LEFT_14_SEQUENCE=TCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_14_SEQUENCE=CGCAATTTGGGTTTTGTTCT
PRIMER_LEFT_14=909,20
PRIMER_RIGHT_14=1800,20
PRIMER_LEFT_14_TM=57.991
PRIMER_RIGHT_14_TM=59.975
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.8000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=892
PRIMER_PAIR_15_PENALTY=2.046072
PRIMER_LEFT_15_PENALTY=2.001949
PRIMER_RIGHT_15_PENALTY=0.044122
PRIMER_LEFT_15_SEQUENCE=AGTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_15_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_15=907,22
PRIMER_RIGHT_15=1552,20
PRIMER_LEFT_15_TM=59.998
PRIMER_RIGHT_15_TM=60.044
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.8000
PRIMER_RIGHT_15_END_STABILITY=11.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=646
PRIMER_PAIR_16_PENALTY=2.053477
PRIMER_LEFT_16_PENALTY=2.009354
PRIMER_RIGHT_16_PENALTY=0.044122
PRIMER_LEFT_16_SEQUENCE=TCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_16_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_16=909,20
PRIMER_RIGHT_16=1552,20
PRIMER_LEFT_16_TM=57.991
PRIMER_RIGHT_16_TM=60.044
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.8000
PRIMER_RIGHT_16_END_STABILITY=11.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=644
PRIMER_PAIR_17_PENALTY=2.059783
PRIMER_LEFT_17_PENALTY=2.034655
PRIMER_RIGHT_17_PENALTY=0.025128
PRIMER_LEFT_17_SEQUENCE=GTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_17_SEQUENCE=CGCAATTTGGGTTTTGTTCT
PRIMER_LEFT_17=908,21
PRIMER_RIGHT_17=1800,20
PRIMER_LEFT_17_TM=58.965
PRIMER_RIGHT_17_TM=59.975
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.8000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=893
PRIMER_PAIR_18_PENALTY=2.078777
PRIMER_LEFT_18_PENALTY=2.034655
PRIMER_RIGHT_18_PENALTY=0.044122
PRIMER_LEFT_18_SEQUENCE=GTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_18_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_18=908,21
PRIMER_RIGHT_18=1552,20
PRIMER_LEFT_18_TM=58.965
PRIMER_RIGHT_18_TM=60.044
PRIMER_LEFT_18_GC_PERCENT=52.381
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.8000
PRIMER_RIGHT_18_END_STABILITY=11.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=645
PRIMER_PAIR_19_PENALTY=2.102942
PRIMER_LEFT_19_PENALTY=0.954019
PRIMER_RIGHT_19_PENALTY=1.148923
PRIMER_LEFT_19_SEQUENCE=CCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_19_SEQUENCE=CATCTTCTCACTGCTTGCTCC
PRIMER_LEFT_19=910,20
PRIMER_RIGHT_19=1880,21
PRIMER_LEFT_19_TM=59.046
PRIMER_RIGHT_19_TM=60.149
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=971
PRIMER_PAIR_20_PENALTY=2.112433
PRIMER_LEFT_20_PENALTY=2.087305
PRIMER_RIGHT_20_PENALTY=0.025128
PRIMER_LEFT_20_SEQUENCE=TCCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_20_SEQUENCE=CGCAATTTGGGTTTTGTTCT
PRIMER_LEFT_20=909,21
PRIMER_RIGHT_20=1800,20
PRIMER_LEFT_20_TM=61.087
PRIMER_RIGHT_20_TM=59.975
PRIMER_LEFT_20_GC_PERCENT=52.381
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=892
PRIMER_PAIR_21_PENALTY=2.130074
PRIMER_LEFT_21_PENALTY=2.085951
PRIMER_RIGHT_21_PENALTY=0.044122
PRIMER_LEFT_21_SEQUENCE=TGTGCCTGAGAGATCTTGTGTT
PRIMER_RIGHT_21_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_21=658,22
PRIMER_RIGHT_21=1552,20
PRIMER_LEFT_21_TM=59.914
PRIMER_RIGHT_21_TM=60.044
PRIMER_LEFT_21_GC_PERCENT=45.455
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=11.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=895
PRIMER_PAIR_22_PENALTY=2.131427
PRIMER_LEFT_22_PENALTY=2.087305
PRIMER_RIGHT_22_PENALTY=0.044122
PRIMER_LEFT_22_SEQUENCE=TCCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_22_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_22=909,21
PRIMER_RIGHT_22=1552,20
PRIMER_LEFT_22_TM=61.087
PRIMER_RIGHT_22_TM=60.044
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=11.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=644
PRIMER_PAIR_23_PENALTY=2.146625
PRIMER_LEFT_23_PENALTY=2.001949
PRIMER_RIGHT_23_PENALTY=0.144675
PRIMER_LEFT_23_SEQUENCE=AGTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_23_SEQUENCE=TCTTCTCACTGCTTGCTCCA
PRIMER_LEFT_23=907,22
PRIMER_RIGHT_23=1878,20
PRIMER_LEFT_23_TM=59.998
PRIMER_RIGHT_23_TM=59.855
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.8000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=972
PRIMER_PAIR_24_PENALTY=2.154030
PRIMER_LEFT_24_PENALTY=2.009354
PRIMER_RIGHT_24_PENALTY=0.144675
PRIMER_LEFT_24_SEQUENCE=TCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_24_SEQUENCE=TCTTCTCACTGCTTGCTCCA
PRIMER_LEFT_24=909,20
PRIMER_RIGHT_24=1878,20
PRIMER_LEFT_24_TM=57.991
PRIMER_RIGHT_24_TM=59.855
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.8000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=970
PRIMER_PAIR_25_PENALTY=2.179330
PRIMER_LEFT_25_PENALTY=2.034655
PRIMER_RIGHT_25_PENALTY=0.144675
PRIMER_LEFT_25_SEQUENCE=GTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_25_SEQUENCE=TCTTCTCACTGCTTGCTCCA
PRIMER_LEFT_25=908,21
PRIMER_RIGHT_25=1878,20
PRIMER_LEFT_25_TM=58.965
PRIMER_RIGHT_25_TM=59.855
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.8000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=971
PRIMER_PAIR_26_PENALTY=2.240063
PRIMER_LEFT_26_PENALTY=0.186600
PRIMER_RIGHT_26_PENALTY=2.053463
PRIMER_LEFT_26_SEQUENCE=TTCCTTCTGGCGATTCTGTT
PRIMER_RIGHT_26_SEQUENCE=TCTCGAAATTCAGTGGGGCT
PRIMER_LEFT_26=706,20
PRIMER_RIGHT_26=1550,20
PRIMER_LEFT_26_TM=59.813
PRIMER_RIGHT_26_TM=62.053
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=10.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=845
PRIMER_PAIR_27_PENALTY=2.244852
PRIMER_LEFT_27_PENALTY=2.200730
PRIMER_RIGHT_27_PENALTY=0.044122
PRIMER_LEFT_27_SEQUENCE=GGCGATTCTGTTCTTCAGCC
PRIMER_RIGHT_27_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_27=714,20
PRIMER_RIGHT_27=1552,20
PRIMER_LEFT_27_TM=62.201
PRIMER_RIGHT_27_TM=60.044
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=11.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=839
PRIMER_PAIR_28_PENALTY=2.285885
PRIMER_LEFT_28_PENALTY=2.260757
PRIMER_RIGHT_28_PENALTY=0.025128
PRIMER_LEFT_28_SEQUENCE=CATCAGTCCTGAGATGGAGTCA
PRIMER_RIGHT_28_SEQUENCE=CGCAATTTGGGTTTTGTTCT
PRIMER_LEFT_28=903,22
PRIMER_RIGHT_28=1800,20
PRIMER_LEFT_28_TM=60.261
PRIMER_RIGHT_28_TM=59.975
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=898
PRIMER_PAIR_29_PENALTY=2.286324
PRIMER_LEFT_29_PENALTY=2.001949
PRIMER_RIGHT_29_PENALTY=0.284374
PRIMER_LEFT_29_SEQUENCE=AGTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_29_SEQUENCE=TTCTCACTGCTTGCTCCAAA
PRIMER_LEFT_29=907,22
PRIMER_RIGHT_29=1876,20
PRIMER_LEFT_29_TM=59.998
PRIMER_RIGHT_29_TM=59.716
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.8000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=970
PRIMER_PAIR_30_PENALTY=2.293729
PRIMER_LEFT_30_PENALTY=2.009354
PRIMER_RIGHT_30_PENALTY=0.284374
PRIMER_LEFT_30_SEQUENCE=TCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_30_SEQUENCE=TTCTCACTGCTTGCTCCAAA
PRIMER_LEFT_30=909,20
PRIMER_RIGHT_30=1876,20
PRIMER_LEFT_30_TM=57.991
PRIMER_RIGHT_30_TM=59.716
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.8000
PRIMER_RIGHT_30_END_STABILITY=8.8000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=968
PRIMER_PAIR_31_PENALTY=2.304879
PRIMER_LEFT_31_PENALTY=2.260757
PRIMER_RIGHT_31_PENALTY=0.044122
PRIMER_LEFT_31_SEQUENCE=CATCAGTCCTGAGATGGAGTCA
PRIMER_RIGHT_31_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_31=903,22
PRIMER_RIGHT_31=1552,20
PRIMER_LEFT_31_TM=60.261
PRIMER_RIGHT_31_TM=60.044
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=11.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=650
PRIMER_PAIR_32_PENALTY=2.315865
PRIMER_LEFT_32_PENALTY=2.271742
PRIMER_RIGHT_32_PENALTY=0.044122
PRIMER_LEFT_32_SEQUENCE=TCCTTCTGGCGATTCTGTTCT
PRIMER_RIGHT_32_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_32=707,21
PRIMER_RIGHT_32=1552,20
PRIMER_LEFT_32_TM=61.272
PRIMER_RIGHT_32_TM=60.044
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=11.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=846
PRIMER_PAIR_33_PENALTY=2.319029
PRIMER_LEFT_33_PENALTY=2.034655
PRIMER_RIGHT_33_PENALTY=0.284374
PRIMER_LEFT_33_SEQUENCE=GTCCTGAGATGGAGTCATGGT
PRIMER_RIGHT_33_SEQUENCE=TTCTCACTGCTTGCTCCAAA
PRIMER_LEFT_33=908,21
PRIMER_RIGHT_33=1876,20
PRIMER_LEFT_33_TM=58.965
PRIMER_RIGHT_33_TM=59.716
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.8000
PRIMER_RIGHT_33_END_STABILITY=8.8000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=969
PRIMER_PAIR_34_PENALTY=2.359972
PRIMER_LEFT_34_PENALTY=2.315850
PRIMER_RIGHT_34_PENALTY=0.044122
PRIMER_LEFT_34_SEQUENCE=GCTTCCTTCTGGCGATTCTG
PRIMER_RIGHT_34_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_34=704,20
PRIMER_RIGHT_34=1552,20
PRIMER_LEFT_34_TM=62.316
PRIMER_RIGHT_34_TM=60.044
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=11.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=849
PRIMER_PAIR_35_PENALTY=2.371679
PRIMER_LEFT_35_PENALTY=2.087305
PRIMER_RIGHT_35_PENALTY=0.284374
PRIMER_LEFT_35_SEQUENCE=TCCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_35_SEQUENCE=TTCTCACTGCTTGCTCCAAA
PRIMER_LEFT_35=909,21
PRIMER_RIGHT_35=1876,20
PRIMER_LEFT_35_TM=61.087
PRIMER_RIGHT_35_TM=59.716
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.8000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=968
PRIMER_PAIR_36_PENALTY=2.391986
PRIMER_LEFT_36_PENALTY=0.186600
PRIMER_RIGHT_36_PENALTY=2.205385
PRIMER_LEFT_36_SEQUENCE=TTCCTTCTGGCGATTCTGTT
PRIMER_RIGHT_36_SEQUENCE=TTTTCTCGAAATTCAGTGGG
PRIMER_LEFT_36=706,20
PRIMER_RIGHT_36=1553,20
PRIMER_LEFT_36_TM=59.813
PRIMER_RIGHT_36_TM=57.795
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=848
PRIMER_PAIR_37_PENALTY=2.393083
PRIMER_LEFT_37_PENALTY=0.339620
PRIMER_RIGHT_37_PENALTY=2.053463
PRIMER_LEFT_37_SEQUENCE=TCCTTCTGGCGATTCTGTTC
PRIMER_RIGHT_37_SEQUENCE=TCTCGAAATTCAGTGGGGCT
PRIMER_LEFT_37=707,20
PRIMER_RIGHT_37=1550,20
PRIMER_LEFT_37_TM=60.340
PRIMER_RIGHT_37_TM=62.053
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=10.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=844
PRIMER_PAIR_38_PENALTY=2.455987
PRIMER_LEFT_38_PENALTY=0.954019
PRIMER_RIGHT_38_PENALTY=1.501968
PRIMER_LEFT_38_SEQUENCE=CCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_38_SEQUENCE=TCGATGATTGGATCCTGAGTT
PRIMER_LEFT_38=910,20
PRIMER_RIGHT_38=1738,21
PRIMER_LEFT_38_TM=59.046
PRIMER_RIGHT_38_TM=59.498
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=829
PRIMER_PAIR_39_PENALTY=2.475609
PRIMER_LEFT_39_PENALTY=2.431486
PRIMER_RIGHT_39_PENALTY=0.044122
PRIMER_LEFT_39_SEQUENCE=TGCCTGAGAGATCTTGTGTTTG
PRIMER_RIGHT_39_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_39=660,22
PRIMER_RIGHT_39=1552,20
PRIMER_LEFT_39_TM=60.431
PRIMER_RIGHT_39_TM=60.044
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=11.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=893
PRIMER_PAIR_40_PENALTY=2.499290
PRIMER_LEFT_40_PENALTY=0.186600
PRIMER_RIGHT_40_PENALTY=2.312690
PRIMER_LEFT_40_SEQUENCE=TTCCTTCTGGCGATTCTGTT
PRIMER_RIGHT_40_SEQUENCE=TTTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_40=706,20
PRIMER_RIGHT_40=1553,21
PRIMER_LEFT_40_TM=59.813
PRIMER_RIGHT_40_TM=61.313
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=11.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=848
PRIMER_PAIR_41_PENALTY=2.520752
PRIMER_LEFT_41_PENALTY=2.476630
PRIMER_RIGHT_41_PENALTY=0.044122
PRIMER_LEFT_41_SEQUENCE=CTGTGCCTGAGAGATCTTGTGT
PRIMER_RIGHT_41_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_41=657,22
PRIMER_RIGHT_41=1552,20
PRIMER_LEFT_41_TM=59.523
PRIMER_RIGHT_41_TM=60.044
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=11.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=896
PRIMER_PAIR_42_PENALTY=2.531959
PRIMER_LEFT_42_PENALTY=0.478496
PRIMER_RIGHT_42_PENALTY=2.053463
PRIMER_LEFT_42_SEQUENCE=TCTGGCGATTCTGTTCTTCA
PRIMER_RIGHT_42_SEQUENCE=TCTCGAAATTCAGTGGGGCT
PRIMER_LEFT_42=711,20
PRIMER_RIGHT_42=1550,20
PRIMER_LEFT_42_TM=59.522
PRIMER_RIGHT_42_TM=62.053
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=10.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=840
PRIMER_PAIR_43_PENALTY=2.545006
PRIMER_LEFT_43_PENALTY=0.339620
PRIMER_RIGHT_43_PENALTY=2.205385
PRIMER_LEFT_43_SEQUENCE=TCCTTCTGGCGATTCTGTTC
PRIMER_RIGHT_43_SEQUENCE=TTTTCTCGAAATTCAGTGGG
PRIMER_LEFT_43=707,20
PRIMER_RIGHT_43=1553,20
PRIMER_LEFT_43_TM=60.340
PRIMER_RIGHT_43_TM=57.795
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=847
PRIMER_PAIR_44_PENALTY=2.545131
PRIMER_LEFT_44_PENALTY=2.260757
PRIMER_RIGHT_44_PENALTY=0.284374
PRIMER_LEFT_44_SEQUENCE=CATCAGTCCTGAGATGGAGTCA
PRIMER_RIGHT_44_SEQUENCE=TTCTCACTGCTTGCTCCAAA
PRIMER_LEFT_44=903,22
PRIMER_RIGHT_44=1876,20
PRIMER_LEFT_44_TM=60.261
PRIMER_RIGHT_44_TM=59.716
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=8.8000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=974
PRIMER_PAIR_45_PENALTY=2.547807
PRIMER_LEFT_45_PENALTY=0.186600
PRIMER_RIGHT_45_PENALTY=2.361206
PRIMER_LEFT_45_SEQUENCE=TTCCTTCTGGCGATTCTGTT
PRIMER_RIGHT_45_SEQUENCE=GAACAATGTCACGAAACTCAGG
PRIMER_LEFT_45=706,20
PRIMER_RIGHT_45=1219,22
PRIMER_LEFT_45_TM=59.813
PRIMER_RIGHT_45_TM=59.639
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=514
PRIMER_PAIR_46_PENALTY=2.593969
PRIMER_LEFT_46_PENALTY=2.549847
PRIMER_RIGHT_46_PENALTY=0.044122
PRIMER_LEFT_46_SEQUENCE=TGTGCCTGAGAGATCTTGTGT
PRIMER_RIGHT_46_SEQUENCE=TTTCTCGAAATTCAGTGGGG
PRIMER_LEFT_46=658,21
PRIMER_RIGHT_46=1552,20
PRIMER_LEFT_46_TM=58.450
PRIMER_RIGHT_46_TM=60.044
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=11.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=895
PRIMER_PAIR_47_PENALTY=2.623963
PRIMER_LEFT_47_PENALTY=0.570500
PRIMER_RIGHT_47_PENALTY=2.053463
PRIMER_LEFT_47_SEQUENCE=CCTTCTGGCGATTCTGTTCT
PRIMER_RIGHT_47_SEQUENCE=TCTCGAAATTCAGTGGGGCT
PRIMER_LEFT_47=708,20
PRIMER_RIGHT_47=1550,20
PRIMER_LEFT_47_TM=59.430
PRIMER_RIGHT_47_TM=62.053
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=10.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=843
PRIMER_PAIR_48_PENALTY=2.623963
PRIMER_LEFT_48_PENALTY=0.570500
PRIMER_RIGHT_48_PENALTY=2.053463
PRIMER_LEFT_48_SEQUENCE=CTTCCTTCTGGCGATTCTGT
PRIMER_RIGHT_48_SEQUENCE=TCTCGAAATTCAGTGGGGCT
PRIMER_LEFT_48=705,20
PRIMER_RIGHT_48=1550,20
PRIMER_LEFT_48_TM=59.430
PRIMER_RIGHT_48_TM=62.053
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=10.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=846
PRIMER_PAIR_49_PENALTY=2.648600
PRIMER_LEFT_49_PENALTY=0.954019
PRIMER_RIGHT_49_PENALTY=1.694581
PRIMER_LEFT_49_SEQUENCE=CCTGAGATGGAGTCATGGTG
PRIMER_RIGHT_49_SEQUENCE=CTTCTCACTGCTTGCTCCAAA
PRIMER_LEFT_49=910,20
PRIMER_RIGHT_49=1877,21
PRIMER_LEFT_49_TM=59.046
PRIMER_RIGHT_49_TM=60.695
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=8.8000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=968
=
SEQUENCE_ID=MAGIC.105_5_11088010_11088015_oy-0_sf-2_16583_8778_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4258926_4258989_mt-0_bur-0_24832_1475_TRUE
SEQUENCE_TARGET=950,163
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_22048627_22049407_bur-0_po-0_171569_58769_TRUE
SEQUENCE_TARGET=950,880
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAGGGGGACAAAATTTTGACTTAACTGACATAAGANATCTAGAAGGTGAAACCACGCGCGTGTGGTAGTCAGAAACGANCAAAGAATCAACCTCGTCCTTGANAATNAAATTCTTGAGATTGGTCACACCAAAANAAAAAAAAAANNNTTTATCATTGCTTCTTCAATAGNATCAACTGACGTAATCATGATAAGTCTACGACAATATCCCTCCACCAAACCTANCCGNTTTNNTTNAACATTTNACAATTATGATGAGTTNAATTTTTTTATTNGAGACTGTTACCACTNNTAGCTTTCATTATCTATAACCNAAGTCTGGTTAATTCATGATTATGTCAACAGTNTNTTTGGTCGAATTTTGATATCGAGTTTGGTTTCTAGGTTTTAGTTTCCTTAAATGTTTGTCTTTACAATGAAGTAAAGTTAAAACAACATTGAGTGAGAAAGTGGCCAACTAATTCTCTCTGTACCATCAATCCAAAATTAAATTGATTTGATTTGATTAATCAAAAAGGTNGTCAGAGAAAAACAAAAGAAAAAAAGGTTTTAAATTTCTTCCCGTACCAGCTAGACCCAGAATCAAAGTAGCATACTTTTGCTACATANNNAAAGTAATTTAACCCCNAAAAACCAAAAANAATGTGTTAGAAAAGCAACAAGAAGAAGAAGAAAGCAAATTAATAGACAAATAATCTTTCTTCTCTGGTTGCTTCTTTTNATTTCTTTCTCTGGAACAGCTTTTCTCTGTTACTTTACTTCTTTGAGAAACTCCATAATCAAAGAGGTAAGTGATTCATGTTGCTTGTGATTATCCCAATGGTTTTGTTCTTTTAGGTTTTCGGAAATAAATAATTATTCTTAATTACCGAGTTGNGTATAATTTGTTTTTGTTAGTTTCTCTGATCTATCCCTTGTCTAAGTTATCTTTTAGGGTAGTTTGTTTTCAGGGAACAANTTTTATCAGTATAATATAGGGAAAATTTTCCAAACAAAATCGATAATAATACTAATATCTGCATAAATACAATTCTAGCCAGTATGTGTTATGATCAGTTNACCTAATTGGGTTAGAAAGATGGAGTTCGATTTAAGATNTCTTAAAAGTTCAAANAAAAACTTTCCTTTTTCATGNAAAGCTCAACAACTTCAAAATCTTTGTTTTCTGGCTGTTTCCTCTTGTCTTCGATTGTTCTTGTTCTTTGGATNTTTTTNTTTTAACTTTTTTTTTGTCTGNAACTGCTGTTTCAATTTGGAATATGTTGCAGGAGTTTTTGAGGTTGAAGCTGTTCCTCCTTGNTAAAACAGAGCAATTATGCTAACTTGCGCCGTCTTTTCAAATCTTGACCATCATCAAAATCATCAGTAAGCTATAACCATCTGTTTATAACCATTTGCTTGAATCAAGAAAAAAAGTTTATCTTCTGTTTCTTGTTATGAACAGTTGAAAGGAATGTTCAAGAAATGGGGAAGAAAGGAAGTTGGTTTTCTGCAATCAAAAGGGTGTTCACACCACATTCCAAAGAGAAGCAGCTAAGCAACAACAACGTAAGAAATTCTTTTAAACACTTTCATTTTGTTTTATAAACTGAAATGATAAATTTTGAGATTGAAGTGTATATACATTTTGGTGTTTGGTTTTGTGTTTTGAAAGCAAGAACCAGAGATAAAGAGTGAAAACAAAGAAAAGAAGAAGAAAGGTTTTGGAAAGAAACTAAGGAATGGAGAAACCAATTCATTTCTTCCCATTTTTAGACAGCCTAGTAGTATCGAAAAGATCTTGTCTGAAGCCGAACGAGAGCATAACCTTGTCTTTAGGCCACCAACTCCCACTNNTCGAGCAAATTCATCATCAACCTCTGTTGCATCTCCTCTTGTTAGGCCTGCTTCTCCTAAAGTGCCTTCTCAAAGATATGTCTCTTCTCCAAAACCCATTTCCCCAAGAGTTGCTTACCCTCAAGTCCATTATCCNAAGCCCCCTTCTCCAAAGCCGCCTTCTCCAAGAGCGGNTTCGCCTAGGATTGTGCAGCGACGTGAGTTTGTGCATAGACCAGAGCCAAGTCTATTGGTCAAAAATGCTTATGCAATTAAGATTCAAGCAGCTTTTAGAGGTTACATGGTAATAATGTAAAACATTCTTGATTCTGTTTTGTCACTATTAATCAAGCAGTAAAATTCATAATTTAGGANATGGAAATTTGTTNGCAGGCAAGGAGGAGTTTCAGAGCTTTGAAAGGTCTAGTCAGGCTTCAAGGNGTGGTAAGAGGACACAGCGTGAAGCGTCAAACGATGAATGCTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_12729541_12729705_wil-2_mt-0_3693_13902_TRUE
SEQUENCE_TARGET=950,264
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNANAANATTACTTTTNTTATATATTTATAAANNAACAATNCNNNTTTTACNNACGTTNAANNNGAAATAATAACANTGTCANNTAATCCTGGTTGAACAATATNAGACGCTTCTAGTCCCATAGGAAACNCACNTTGTCATGTATTATTTTTNGTCAAATGATCTGANCAAACGTCNGTCTCATCGAACTCAAAGTNGTNTGTTTCTGANTCTGNATCNGTTGCGACAAAGTATACAAAATTTCCTTCGATATCAGGTAGCAAANCANCTCCTGATAAGTGNTATATTTNATATATAGTTNTTCGAAGTTTATTAATAANNGANATGTGCTAGAGAANATTNTAATGAACAATNAATATATAACAAATANCAACCATNTANTAGTTTCTTTGAAGCTGCTCNTTTCCTCTTAAGAACATTTATNCNAAACCTCCTCGCATCTTNNNNNNTGTTTNTCTTGCNGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14345522_14345570_no-0_can-0_16649_29350_TRUE
SEQUENCE_TARGET=950,148
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4947636_4947938_oy-0_wu-0_184192_437161_TRUE
SEQUENCE_TARGET=950,402
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_9304869_9304970_hi-0_ct-1_333_307933_TRUE
SEQUENCE_TARGET=950,201
SEQUENCE_TEMPLATE=TTATCGGCTTTCGATTGGNTTCAATAATTTTTCATTTTTGGCTCGATTCGAGCTNAGTATCAGTTCTTTTGTAGAAANATAACACTATTGTTGTATATGTATTNAAGTTTGATTGAGTTTCACCACTGAATCTAATGTTTTAATCTACTTTCTAATTTGCTTAATGAATTGCTAATTTAGTTTTGTTCATAAACTTCTTGTTTCACCAATTCTTTATTTGTACCNTCTTGGNTAANAAATGTNTTACACAATGACTATGTAACTTTACATTTCAATAGATTTAGTTTCGTCGGGAAAAAAAGACGATATCAAGAATAAATATNTGTGAATGAGNTTTTGGGCCTACGNGCTTTCTTTATCTCTGCTTCAATTTGATTNACTAGTGGACTTNNTCAAGAGTTNTCATGCTACACTNAAATATGTTGCATGAATCTTTAACAAAAGANANGTTGATANAGCAGCAAAAAGGTGATAAAACTCTTGTATGAAAANTGNACATACTAGTGATTAAATCAATTTGCATCCAAACTNACANNNNNTTTTTTTNNGTNGTCGATCCAAACTCACATATATTGATAAAAGANCCACANTTNTACGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNTNNTNNNGNTTTNNNNNTNAANTTNNNNNNNCCNANANTNNAANATACNNTNNCTTTTNTNAANCNNANTANCTANAANNNAATNTNCNNTCNANNNAGCCCAANANNTANNANNNNANNNCCAATCAAGTNAGCNNAAGTNNCNATNACTNNNNCCCNNAANANNNCAAGNNTCANATGNNNCNAANNCNANNATNAANANNTCNNNANTNNNAANAAAANANNTNNNCANNGTCAACNANNNACNNNAGNGNCAANNTANNTANNTNTNNTGNNANNNTANANATANTNNANNNTTANNNTANNCNNAANNANNNNNTNTTTCATTTAGNNNTNANNNNAANATTNNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCGANACACNTTTTCTNTCCNNNNNNNNNNNNNNNNGTCTCCCACGATTTATNTGNTTCTATTTTTCCACTANNNNTTTNTTTCTGATTTGAGNGTTGGAGAANNGNAAGAAAACATNTTCCAATCATATATTTNTCTGCTCNTGCGTGTNNNTTGTGATGTAGGAGCCTTGTCTTCAATAGACAAACCGCACGACAAAAACTCCCCGAAACTTANNNTAATAATNTTGNATTTTNGTNAGTTGNTTATTAACANTCAAGTAANNNNNNATATTCGTTNAAANTGGTANATGATTTCTATTTNAAAAGTGCTTTTATGATATGATATAAAAGATTGTTATTTGATTTCTCTNACTATTGATTTATANNTATGATCCTATAAACATTTTAAGCATTTCCAAAATTTCATNAGGCTAGNGTTTNTTTTCTTACTTACCAATTTGTTTCTATATNTTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCCTCCATANTTAGGCATGGTCCTTNAAAAAAATGGTCAATGCATATTTTTGCCAAACGTATCANTGTGTCATCNTNTCTTCATGTTCCGATTCACATAGCCTNTGCTTTTATGTTTNTAAAAAGATACTTGGCGTGTACACAANGGNCAGCTTTCCACAAAGAGNTTTTGAATTACGTCATATCTTTATAGGATTCACATCTATTCTATCAATTCATANGAACTTTTTCANANTTTATTTCATTTGCTAATCTCCAAAAAGATTTCTAATCACATGATCATTTAAGAAATGTGTTNTTTATTTATTTAATTTGAATCAAAATCCATTTTCTTCNTCCATACNCTACCAAAAGAATATCTTGATCGAATGGCTAATTTAATGTAGANTGTTATTCAAGGAAAATTAGCCACGATTATCGTAATCTCNAAATTCTGTTCACACANTTATNTTATCTAATANNNTAGTACTCNATATAACTTCATTTATTATATTTGCTTCTTTGATTAGCNTTTTCCCAATGTCTTCCTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.304343
PRIMER_LEFT_0_PENALTY=4.150321
PRIMER_RIGHT_0_PENALTY=0.154023
PRIMER_LEFT_0_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_0_SEQUENCE=GAGTTTTTGTCGTGCGGTTT
PRIMER_LEFT_0=272,22
PRIMER_RIGHT_0=1271,20
PRIMER_LEFT_0_TM=57.850
PRIMER_RIGHT_0_TM=60.154
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=11.4000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=1000
PRIMER_PAIR_1_PENALTY=4.951409
PRIMER_LEFT_1_PENALTY=4.150321
PRIMER_RIGHT_1_PENALTY=0.801088
PRIMER_LEFT_1_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_1_SEQUENCE=TGTCGTGCGGTTTGTCTATT
PRIMER_LEFT_1=272,22
PRIMER_RIGHT_1=1264,20
PRIMER_LEFT_1_TM=57.850
PRIMER_RIGHT_1_TM=59.199
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=993
PRIMER_PAIR_2_PENALTY=5.114029
PRIMER_LEFT_2_PENALTY=4.150321
PRIMER_RIGHT_2_PENALTY=0.963709
PRIMER_LEFT_2_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_2_SEQUENCE=GTTTTTGTCGTGCGGTTTGT
PRIMER_LEFT_2=272,22
PRIMER_RIGHT_2=1269,20
PRIMER_LEFT_2_TM=57.850
PRIMER_RIGHT_2_TM=60.964
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=998
PRIMER_PAIR_3_PENALTY=5.238475
PRIMER_LEFT_3_PENALTY=4.150321
PRIMER_RIGHT_3_PENALTY=1.088154
PRIMER_LEFT_3_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_3_SEQUENCE=AGTTTTTGTCGTGCGGTTTG
PRIMER_LEFT_3=272,22
PRIMER_RIGHT_3=1270,20
PRIMER_LEFT_3_TM=57.850
PRIMER_RIGHT_3_TM=61.088
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=999
PRIMER_PAIR_4_PENALTY=5.699259
PRIMER_LEFT_4_PENALTY=4.150321
PRIMER_RIGHT_4_PENALTY=1.548938
PRIMER_LEFT_4_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_4_SEQUENCE=TTGTCGTGCGGTTTGTCTATT
PRIMER_LEFT_4=272,22
PRIMER_RIGHT_4=1265,21
PRIMER_LEFT_4_TM=57.850
PRIMER_RIGHT_4_TM=60.549
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=994
PRIMER_PAIR_5_PENALTY=5.785956
PRIMER_LEFT_5_PENALTY=4.150321
PRIMER_RIGHT_5_PENALTY=1.635635
PRIMER_LEFT_5_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_5_SEQUENCE=TTTTGTCGTGCGGTTTGTCT
PRIMER_LEFT_5=272,22
PRIMER_RIGHT_5=1267,20
PRIMER_LEFT_5_TM=57.850
PRIMER_RIGHT_5_TM=61.636
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=11.4000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=996
PRIMER_PAIR_6_PENALTY=5.824218
PRIMER_LEFT_6_PENALTY=4.150321
PRIMER_RIGHT_6_PENALTY=1.673897
PRIMER_LEFT_6_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_6_SEQUENCE=CGTGCGGTTTGTCTATTGAAG
PRIMER_LEFT_6=272,22
PRIMER_RIGHT_6=1261,21
PRIMER_LEFT_6_TM=57.850
PRIMER_RIGHT_6_TM=60.674
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=11.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=990
PRIMER_PAIR_7_PENALTY=6.152999
PRIMER_LEFT_7_PENALTY=4.150321
PRIMER_RIGHT_7_PENALTY=2.002679
PRIMER_LEFT_7_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_7_SEQUENCE=TTTTTGTCGTGCGGTTTGTC
PRIMER_LEFT_7=272,22
PRIMER_RIGHT_7=1268,20
PRIMER_LEFT_7_TM=57.850
PRIMER_RIGHT_7_TM=62.003
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=11.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=997
PRIMER_PAIR_8_PENALTY=6.987454
PRIMER_LEFT_8_PENALTY=4.150321
PRIMER_RIGHT_8_PENALTY=2.837134
PRIMER_LEFT_8_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_8_SEQUENCE=AGTTTTTGTCGTGCGGTTTGT
PRIMER_LEFT_8=272,22
PRIMER_RIGHT_8=1270,21
PRIMER_LEFT_8_TM=57.850
PRIMER_RIGHT_8_TM=61.837
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=11.4000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=999
PRIMER_PAIR_9_PENALTY=7.285604
PRIMER_LEFT_9_PENALTY=4.150321
PRIMER_RIGHT_9_PENALTY=3.135283
PRIMER_LEFT_9_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_9_SEQUENCE=AGACAAGGCTCCTACATCACAA
PRIMER_LEFT_9=272,22
PRIMER_RIGHT_9=1242,22
PRIMER_LEFT_9_TM=57.850
PRIMER_RIGHT_9_TM=58.865
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=11.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=971
PRIMER_PAIR_10_PENALTY=7.285604
PRIMER_LEFT_10_PENALTY=4.150321
PRIMER_RIGHT_10_PENALTY=3.135283
PRIMER_LEFT_10_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_10_SEQUENCE=AAGACAAGGCTCCTACATCACA
PRIMER_LEFT_10=272,22
PRIMER_RIGHT_10=1243,22
PRIMER_LEFT_10_TM=57.850
PRIMER_RIGHT_10_TM=58.865
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=45.455
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=972
PRIMER_PAIR_11_PENALTY=7.328747
PRIMER_LEFT_11_PENALTY=4.150321
PRIMER_RIGHT_11_PENALTY=3.178426
PRIMER_LEFT_11_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_11_SEQUENCE=AAGACAAGGCTCCTACATCACAA
PRIMER_LEFT_11=272,22
PRIMER_RIGHT_11=1243,23
PRIMER_LEFT_11_TM=57.850
PRIMER_RIGHT_11_TM=60.178
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=11.4000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=7.339662
PRIMER_LEFT_12_PENALTY=4.150321
PRIMER_RIGHT_12_PENALTY=3.189342
PRIMER_LEFT_12_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_12_SEQUENCE=GACAAGGCTCCTACATCACAA
PRIMER_LEFT_12=272,22
PRIMER_RIGHT_12=1241,21
PRIMER_LEFT_12_TM=57.850
PRIMER_RIGHT_12_TM=57.811
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=11.4000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=970
PRIMER_PAIR_13_PENALTY=7.688498
PRIMER_LEFT_13_PENALTY=4.150321
PRIMER_RIGHT_13_PENALTY=3.538178
PRIMER_LEFT_13_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_13_SEQUENCE=TTGAAGACAAGGCTCCTACATC
PRIMER_LEFT_13=272,22
PRIMER_RIGHT_13=1246,22
PRIMER_LEFT_13_TM=57.850
PRIMER_RIGHT_13_TM=58.462
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=975
PRIMER_PAIR_14_PENALTY=7.749861
PRIMER_LEFT_14_PENALTY=4.150321
PRIMER_RIGHT_14_PENALTY=3.599540
PRIMER_LEFT_14_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_14_SEQUENCE=AGACAAGGCTCCTACATCACA
PRIMER_LEFT_14=272,22
PRIMER_RIGHT_14=1242,21
PRIMER_LEFT_14_TM=57.850
PRIMER_RIGHT_14_TM=57.400
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.4000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=971
PRIMER_PAIR_15_PENALTY=7.825545
PRIMER_LEFT_15_PENALTY=4.150321
PRIMER_RIGHT_15_PENALTY=3.675224
PRIMER_LEFT_15_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_15_SEQUENCE=GAAGACAAGGCTCCTACATCACA
PRIMER_LEFT_15=272,22
PRIMER_RIGHT_15=1244,23
PRIMER_LEFT_15_TM=57.850
PRIMER_RIGHT_15_TM=60.675
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=973
PRIMER_PAIR_16_PENALTY=7.825545
PRIMER_LEFT_16_PENALTY=4.150321
PRIMER_RIGHT_16_PENALTY=3.675224
PRIMER_LEFT_16_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_16_SEQUENCE=TGAAGACAAGGCTCCTACATCAC
PRIMER_LEFT_16=272,22
PRIMER_RIGHT_16=1245,23
PRIMER_LEFT_16_TM=57.850
PRIMER_RIGHT_16_TM=60.675
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=11.4000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=974
PRIMER_PAIR_17_PENALTY=7.879075
PRIMER_LEFT_17_PENALTY=4.150321
PRIMER_RIGHT_17_PENALTY=3.728755
PRIMER_LEFT_17_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_17_SEQUENCE=GTTTTTGTCGTGCGGTTTGTC
PRIMER_LEFT_17=272,22
PRIMER_RIGHT_17=1269,21
PRIMER_LEFT_17_TM=57.850
PRIMER_RIGHT_17_TM=62.729
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=11.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=998
PRIMER_PAIR_18_PENALTY=7.912098
PRIMER_LEFT_18_PENALTY=4.150321
PRIMER_RIGHT_18_PENALTY=3.761778
PRIMER_LEFT_18_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_18_SEQUENCE=TTTGTCGTGCGGTTTGTCTATT
PRIMER_LEFT_18=272,22
PRIMER_RIGHT_18=1266,22
PRIMER_LEFT_18_TM=57.850
PRIMER_RIGHT_18_TM=61.762
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=11.4000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=995
PRIMER_PAIR_19_PENALTY=7.943984
PRIMER_LEFT_19_PENALTY=4.150321
PRIMER_RIGHT_19_PENALTY=3.793663
PRIMER_LEFT_19_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_19_SEQUENCE=GACAAGGCTCCTACATCACA
PRIMER_LEFT_19=272,22
PRIMER_RIGHT_19=1241,20
PRIMER_LEFT_19_TM=57.850
PRIMER_RIGHT_19_TM=56.206
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=11.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=970
PRIMER_PAIR_20_PENALTY=7.986272
PRIMER_LEFT_20_PENALTY=4.150321
PRIMER_RIGHT_20_PENALTY=3.835952
PRIMER_LEFT_20_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_20_SEQUENCE=TTTTTGTCGTGCGGTTTGTCT
PRIMER_LEFT_20=272,22
PRIMER_RIGHT_20=1268,21
PRIMER_LEFT_20_TM=57.850
PRIMER_RIGHT_20_TM=62.836
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=11.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=997
PRIMER_PAIR_21_PENALTY=7.986272
PRIMER_LEFT_21_PENALTY=4.150321
PRIMER_RIGHT_21_PENALTY=3.835952
PRIMER_LEFT_21_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_21_SEQUENCE=GAGTTTTTGTCGTGCGGTTTG
PRIMER_LEFT_21=272,22
PRIMER_RIGHT_21=1271,21
PRIMER_LEFT_21_TM=57.850
PRIMER_RIGHT_21_TM=62.836
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=11.4000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=1000
PRIMER_PAIR_22_PENALTY=8.086962
PRIMER_LEFT_22_PENALTY=4.150321
PRIMER_RIGHT_22_PENALTY=3.936642
PRIMER_LEFT_22_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_22_SEQUENCE=TTGAAGACAAGGCTCCTACA
PRIMER_LEFT_22=272,22
PRIMER_RIGHT_22=1246,20
PRIMER_LEFT_22_TM=57.850
PRIMER_RIGHT_22_TM=56.063
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=11.4000
PRIMER_RIGHT_22_END_STABILITY=5.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=975
PRIMER_PAIR_23_PENALTY=8.150363
PRIMER_LEFT_23_PENALTY=4.150321
PRIMER_RIGHT_23_PENALTY=4.000042
PRIMER_LEFT_23_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_23_SEQUENCE=GAAGACAAGGCTCCTACATCA
PRIMER_LEFT_23=272,22
PRIMER_RIGHT_23=1244,21
PRIMER_LEFT_23_TM=57.850
PRIMER_RIGHT_23_TM=57.000
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=11.4000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=973
PRIMER_PAIR_24_PENALTY=8.150363
PRIMER_LEFT_24_PENALTY=4.150321
PRIMER_RIGHT_24_PENALTY=4.000042
PRIMER_LEFT_24_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_24_SEQUENCE=TGAAGACAAGGCTCCTACATC
PRIMER_LEFT_24=272,22
PRIMER_RIGHT_24=1245,21
PRIMER_LEFT_24_TM=57.850
PRIMER_RIGHT_24_TM=57.000
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=11.4000
PRIMER_RIGHT_24_END_STABILITY=6.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=974
PRIMER_PAIR_25_PENALTY=8.215042
PRIMER_LEFT_25_PENALTY=4.150321
PRIMER_RIGHT_25_PENALTY=4.064721
PRIMER_LEFT_25_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_25_SEQUENCE=GAAGACAAGGCTCCTACATCAC
PRIMER_LEFT_25=272,22
PRIMER_RIGHT_25=1244,22
PRIMER_LEFT_25_TM=57.850
PRIMER_RIGHT_25_TM=57.935
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=11.4000
PRIMER_RIGHT_25_END_STABILITY=6.3000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=973
PRIMER_PAIR_26_PENALTY=8.238258
PRIMER_LEFT_26_PENALTY=4.150321
PRIMER_RIGHT_26_PENALTY=4.087937
PRIMER_LEFT_26_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_26_SEQUENCE=GTGCGGTTTGTCTATTGAAG
PRIMER_LEFT_26=272,22
PRIMER_RIGHT_26=1260,20
PRIMER_LEFT_26_TM=57.850
PRIMER_RIGHT_26_TM=55.912
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=11.4000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=989
PRIMER_PAIR_27_PENALTY=8.354559
PRIMER_LEFT_27_PENALTY=4.150321
PRIMER_RIGHT_27_PENALTY=4.204238
PRIMER_LEFT_27_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_27_SEQUENCE=ATTGAAGACAAGGCTCCTACATC
PRIMER_LEFT_27=272,22
PRIMER_RIGHT_27=1247,23
PRIMER_LEFT_27_TM=57.850
PRIMER_RIGHT_27_TM=58.796
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=11.4000
PRIMER_RIGHT_27_END_STABILITY=6.3000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=976
PRIMER_PAIR_28_PENALTY=8.396823
PRIMER_LEFT_28_PENALTY=4.150321
PRIMER_RIGHT_28_PENALTY=4.246502
PRIMER_LEFT_28_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_28_SEQUENCE=ACAAGGCTCCTACATCACAA
PRIMER_LEFT_28=272,22
PRIMER_RIGHT_28=1240,20
PRIMER_LEFT_28_TM=57.850
PRIMER_RIGHT_28_TM=55.753
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=11.4000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=969
PRIMER_PAIR_29_PENALTY=8.547226
PRIMER_LEFT_29_PENALTY=4.150321
PRIMER_RIGHT_29_PENALTY=4.396905
PRIMER_LEFT_29_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_29_SEQUENCE=TCGTGCGGTTTGTCTATTGAAG
PRIMER_LEFT_29=272,22
PRIMER_RIGHT_29=1262,22
PRIMER_LEFT_29_TM=57.850
PRIMER_RIGHT_29_TM=62.397
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=11.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=7.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=991
PRIMER_PAIR_30_PENALTY=8.593144
PRIMER_LEFT_30_PENALTY=4.150321
PRIMER_RIGHT_30_PENALTY=4.442823
PRIMER_LEFT_30_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_30_SEQUENCE=ATTGAAGACAAGGCTCCTACA
PRIMER_LEFT_30=272,22
PRIMER_RIGHT_30=1247,21
PRIMER_LEFT_30_TM=57.850
PRIMER_RIGHT_30_TM=56.557
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=11.4000
PRIMER_RIGHT_30_END_STABILITY=5.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=976
PRIMER_PAIR_31_PENALTY=9.165900
PRIMER_LEFT_31_PENALTY=4.150321
PRIMER_RIGHT_31_PENALTY=5.015579
PRIMER_LEFT_31_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_31_SEQUENCE=AAGACAAGGCTCCTACATCAC
PRIMER_LEFT_31=272,22
PRIMER_RIGHT_31=1243,21
PRIMER_LEFT_31_TM=57.850
PRIMER_RIGHT_31_TM=55.984
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=11.4000
PRIMER_RIGHT_31_END_STABILITY=6.3000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=972
PRIMER_PAIR_32_PENALTY=9.303382
PRIMER_LEFT_32_PENALTY=4.150321
PRIMER_RIGHT_32_PENALTY=5.153061
PRIMER_LEFT_32_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_32_SEQUENCE=TATTGAAGACAAGGCTCCTACATC
PRIMER_LEFT_32=272,22
PRIMER_RIGHT_32=1248,24
PRIMER_LEFT_32_TM=57.850
PRIMER_RIGHT_32_TM=58.847
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=41.667
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=11.4000
PRIMER_RIGHT_32_END_STABILITY=6.3000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=977
PRIMER_PAIR_33_PENALTY=9.341322
PRIMER_LEFT_33_PENALTY=4.150321
PRIMER_RIGHT_33_PENALTY=5.191001
PRIMER_LEFT_33_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_33_SEQUENCE=TGTCTATTGAAGACAAGGCTCCTAC
PRIMER_LEFT_33=272,22
PRIMER_RIGHT_33=1252,25
PRIMER_LEFT_33_TM=57.850
PRIMER_RIGHT_33_TM=60.191
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=44.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=11.4000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=981
PRIMER_PAIR_34_PENALTY=9.433355
PRIMER_LEFT_34_PENALTY=4.150321
PRIMER_RIGHT_34_PENALTY=5.283034
PRIMER_LEFT_34_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_34_SEQUENCE=CTATTGAAGACAAGGCTCCTACATC
PRIMER_LEFT_34=272,22
PRIMER_RIGHT_34=1249,25
PRIMER_LEFT_34_TM=57.850
PRIMER_RIGHT_34_TM=59.717
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=44.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=11.4000
PRIMER_RIGHT_34_END_STABILITY=6.3000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=978
PRIMER_PAIR_35_PENALTY=9.649924
PRIMER_LEFT_35_PENALTY=4.150321
PRIMER_RIGHT_35_PENALTY=5.499603
PRIMER_LEFT_35_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_35_SEQUENCE=GTTTTTGTCGTGCGGTTTGTCT
PRIMER_LEFT_35=272,22
PRIMER_RIGHT_35=1269,22
PRIMER_LEFT_35_TM=57.850
PRIMER_RIGHT_35_TM=63.500
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=11.4000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=998
PRIMER_PAIR_36_PENALTY=9.649924
PRIMER_LEFT_36_PENALTY=4.150321
PRIMER_RIGHT_36_PENALTY=5.499603
PRIMER_LEFT_36_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_36_SEQUENCE=AGTTTTTGTCGTGCGGTTTGTC
PRIMER_LEFT_36=272,22
PRIMER_RIGHT_36=1270,22
PRIMER_LEFT_36_TM=57.850
PRIMER_RIGHT_36_TM=63.500
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=11.4000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=999
PRIMER_PAIR_37_PENALTY=9.649924
PRIMER_LEFT_37_PENALTY=4.150321
PRIMER_RIGHT_37_PENALTY=5.499603
PRIMER_LEFT_37_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_37_SEQUENCE=GAGTTTTTGTCGTGCGGTTTGT
PRIMER_LEFT_37=272,22
PRIMER_RIGHT_37=1271,22
PRIMER_LEFT_37_TM=57.850
PRIMER_RIGHT_37_TM=63.500
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=11.4000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=1000
PRIMER_PAIR_38_PENALTY=10.011876
PRIMER_LEFT_38_PENALTY=4.150321
PRIMER_RIGHT_38_PENALTY=5.861555
PRIMER_LEFT_38_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_38_SEQUENCE=GAAGACAAGGCTCCTACATCACAA
PRIMER_LEFT_38=272,22
PRIMER_RIGHT_38=1244,24
PRIMER_LEFT_38_TM=57.850
PRIMER_RIGHT_38_TM=61.862
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=45.833
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=11.4000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=973
PRIMER_PAIR_39_PENALTY=10.011876
PRIMER_LEFT_39_PENALTY=4.150321
PRIMER_RIGHT_39_PENALTY=5.861555
PRIMER_LEFT_39_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_39_SEQUENCE=TTGAAGACAAGGCTCCTACATCAC
PRIMER_LEFT_39=272,22
PRIMER_RIGHT_39=1246,24
PRIMER_LEFT_39_TM=57.850
PRIMER_RIGHT_39_TM=61.862
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=45.833
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=11.4000
PRIMER_RIGHT_39_END_STABILITY=6.3000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=975
PRIMER_PAIR_40_PENALTY=10.213140
PRIMER_LEFT_40_PENALTY=4.150321
PRIMER_RIGHT_40_PENALTY=6.062819
PRIMER_LEFT_40_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_40_SEQUENCE=GTCGTGCGGTTTGTCTATTGAAG
PRIMER_LEFT_40=272,22
PRIMER_RIGHT_40=1263,23
PRIMER_LEFT_40_TM=57.850
PRIMER_RIGHT_40_TM=63.063
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=47.826
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=11.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=992
PRIMER_PAIR_41_PENALTY=10.287756
PRIMER_LEFT_41_PENALTY=4.150321
PRIMER_RIGHT_41_PENALTY=6.137436
PRIMER_LEFT_41_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_41_SEQUENCE=TCTATTGAAGACAAGGCTCCTAC
PRIMER_LEFT_41=272,22
PRIMER_RIGHT_41=1250,23
PRIMER_LEFT_41_TM=57.850
PRIMER_RIGHT_41_TM=56.863
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=43.478
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=11.4000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=979
PRIMER_PAIR_42_PENALTY=10.440330
PRIMER_LEFT_42_PENALTY=4.150321
PRIMER_RIGHT_42_PENALTY=6.290009
PRIMER_LEFT_42_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_42_SEQUENCE=GTCTATTGAAGACAAGGCTCCTAC
PRIMER_LEFT_42=272,22
PRIMER_RIGHT_42=1251,24
PRIMER_LEFT_42_TM=57.850
PRIMER_RIGHT_42_TM=57.710
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=45.833
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=11.4000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=980
PRIMER_PAIR_43_PENALTY=11.136827
PRIMER_LEFT_43_PENALTY=4.150321
PRIMER_RIGHT_43_PENALTY=6.986507
PRIMER_LEFT_43_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_43_SEQUENCE=CTATTGAAGACAAGGCTCCTAC
PRIMER_LEFT_43=272,22
PRIMER_RIGHT_43=1249,22
PRIMER_LEFT_43_TM=57.850
PRIMER_RIGHT_43_TM=55.013
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.4000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=978
PRIMER_PAIR_44_PENALTY=11.164681
PRIMER_LEFT_44_PENALTY=4.150321
PRIMER_RIGHT_44_PENALTY=7.014360
PRIMER_LEFT_44_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_44_SEQUENCE=ATTGAAGACAAGGCTCCTACATCAC
PRIMER_LEFT_44=272,22
PRIMER_RIGHT_44=1247,25
PRIMER_LEFT_44_TM=57.850
PRIMER_RIGHT_44_TM=62.014
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=44.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=11.4000
PRIMER_RIGHT_44_END_STABILITY=6.3000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=976
PRIMER_PAIR_45_PENALTY=11.355814
PRIMER_LEFT_45_PENALTY=4.150321
PRIMER_RIGHT_45_PENALTY=7.205493
PRIMER_LEFT_45_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_45_SEQUENCE=AGTTTTTGTCGTGCGGTTTGTCT
PRIMER_LEFT_45=272,22
PRIMER_RIGHT_45=1270,23
PRIMER_LEFT_45_TM=57.850
PRIMER_RIGHT_45_TM=64.205
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=43.478
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=11.4000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=999
PRIMER_PAIR_46_PENALTY=11.414847
PRIMER_LEFT_46_PENALTY=4.150321
PRIMER_RIGHT_46_PENALTY=7.264526
PRIMER_LEFT_46_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_46_SEQUENCE=TGAAGACAAGGCTCCTACATCACA
PRIMER_LEFT_46=272,22
PRIMER_RIGHT_46=1245,24
PRIMER_LEFT_46_TM=57.850
PRIMER_RIGHT_46_TM=63.265
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=45.833
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=11.4000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=974
PRIMER_PAIR_47_PENALTY=11.418400
PRIMER_LEFT_47_PENALTY=4.150321
PRIMER_RIGHT_47_PENALTY=7.268079
PRIMER_LEFT_47_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_47_SEQUENCE=TCTATTGAAGACAAGGCTCCTACATC
PRIMER_LEFT_47=272,22
PRIMER_RIGHT_47=1250,26
PRIMER_LEFT_47_TM=57.850
PRIMER_RIGHT_47_TM=61.268
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=42.308
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=11.4000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=7.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=979
PRIMER_PAIR_48_PENALTY=12.108520
PRIMER_LEFT_48_PENALTY=4.150321
PRIMER_RIGHT_48_PENALTY=7.958199
PRIMER_LEFT_48_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_48_SEQUENCE=TATTGAAGACAAGGCTCCTACATCAC
PRIMER_LEFT_48=272,22
PRIMER_RIGHT_48=1248,26
PRIMER_LEFT_48_TM=57.850
PRIMER_RIGHT_48_TM=61.958
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=42.308
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=11.4000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=977
PRIMER_PAIR_49_PENALTY=13.046788
PRIMER_LEFT_49_PENALTY=4.150321
PRIMER_RIGHT_49_PENALTY=8.896467
PRIMER_LEFT_49_SEQUENCE=CAATAGATTTAGTTTCGTCGGG
PRIMER_RIGHT_49_SEQUENCE=GTCTATTGAAGACAAGGCTCCTACATC
PRIMER_LEFT_49=272,22
PRIMER_RIGHT_49=1251,27
PRIMER_LEFT_49_TM=57.850
PRIMER_RIGHT_49_TM=61.896
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=44.444
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=11.4000
PRIMER_RIGHT_49_END_STABILITY=6.3000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=980
=
SEQUENCE_ID=MAGIC.446_2_6666284_6666285_rsch-4_po-0_12372_31510_TRUE
SEQUENCE_TARGET=950,101
SEQUENCE_TEMPLATE=TAATANAATGGCNGACAAAACAAAAGCAAAATCGTTTCACGTAGTCATGGAAGCTATAACGGACGGTTCTGCATGTACATGGAAGCTATCTATTGTAATTGCTTATGTTGTAGTTAGTCTATCATCATANNNNNNNNNNNNNNNNNNNNNNNNTGATGANATAATATATGNCNAAAAAAATCAAAGAATACCTATAATTATTATTTTTGTTTTGCTTTGAANGAGCCAAAATAGTTTNTTTTTTNNCCAAAATCAGAGANNCTCAAGAGGGTCGATGTCAGANGAAGACTTGGTTTATACAAAATNACTTTGTTTTTAGACATCTATCGNTTAATTAANNATATNCATATGGTACAANNACTTGTTTAGAGATTCTCAANACTGCCTGGTCCACTAGAACTGGATAGTNTAAAATACACATATGTTTGATTAAGAAATTNAATATTTCATTTTATTAATNCNNTTTCANTTAGTTTATNNTNTNANGTTGCANTAGAATNATCNGTNAAGTTTAAATTTNNGTCATTNTANAGNCATTNATTNATATTTTGCTTTCANCCACAACTCATTTANNTTTTAAATNGTTCNANCTGTCNAAGNANACAAAANTNTCTNNANANCANATTGNTTTTTATTTANCATTCANNANCTATANTTNGTAATNGTGNANATCNAAAAAAATAAAAGAAAANCGNNNNNNNNNNNCTGCGAACCNTNTTTAAACNAANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNGACTNGACNNCAATTAGGNTTTGAATNNATNTTGTGGTTAGTGCNNACTAATCCNTNANCNGTCGANTCNATANNNNNNNNNNNNNNNNNNNNNNNNNCNANNAATTANTTTAAANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTNCGCACNANNATATANTATATATAANTTACNAATANTAAATAAAAANNNATNNNATCTATTGANNGTNCTNTNCNNNNAGANCNNNGTAACCATTCNAANCNTTANTATACTANCTAATTTAAATCTCAATAAATATATAAAANCACACAATNCAACNAAATAAATACTATAAGTTCCGNTCCAATNTTTGTAANTTTATTTGTGTACATACGAAGATCTCGANGAATATAGATATTAGTATTTCTCCAAGACTATAGAGTAAACTCATGACTAAAAGCATTATTTTACAAAATCACTGACACAAAAAGTCACCGACACCGACAAAACTAAGACTTTGGTTTTCCACCATAAGCTTGCTTACAAATGTAAAATGGTTTCTGCGATGGAGCACACATCTTAGCTTTCCCTGGACATCCCTGTAGACAATTATTCCCCAAACTTCCCATTACTTCTGCGTCTNTTCTCACAAATCTNGCCGGAGGTTTTCCCGTCAAGAAGTTCCCGTCTAAGTAAAGCCGTCTTAATGTCTTGCTCCGTTCGTATTCCGACGGTATAACACCGTGAAGCACGTTGTATCTCATAGANAGTGAAGAAAGTCTTGGATACGCCGCGAAACTCGCCGGAGCGTTTCCTCTGATTTGGTTGAATCCTAGCTCTACAGCTACGAGGTTGTTTTCTCCGGCGAGGTTTGGAGGGAGGACTTCGATGCCTGTGAGAGTGTTGTTCGCTAGATCGACTTGTTGGATTGATTCAAGTAGGAAGAACCAAGCTCCGAGTGTTCCGGTGAATGAGTTCTCAGCGANTTCNACGATTTCGAGCTGAGTTGACTCGGTGAATGAGTCTTTTGAGATGGGTCCTGATAAAGTATTCGCCTTTAAAGCTAAATCGATGAGGTTTTTCGGAAGCTTTGGGATGGCTCCGGTGAGCTTGTTGTAGCTAAGATCGAGTTGTCGGAGATTTGAAAGCGAGTTCATTGTTTTTGGAAGCGGCCCGGTGAGAGAGTTGTGAGATATGTCAATGGATTCGAGTGAGTTGAGTCGAGTTACTGAGTCGGGTAATGACCCGGAAAACGAGTTGGATCGGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.634656
PRIMER_LEFT_0_PENALTY=1.622186
PRIMER_RIGHT_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_0_SEQUENCE=GTCGGTGTCGGTGACTTTTT
PRIMER_LEFT_0=261,20
PRIMER_RIGHT_0=1238,20
PRIMER_LEFT_0_TM=58.378
PRIMER_RIGHT_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=978
PRIMER_PAIR_1_PENALTY=2.012799
PRIMER_LEFT_1_PENALTY=1.622186
PRIMER_RIGHT_1_PENALTY=0.390614
PRIMER_LEFT_1_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_1_SEQUENCE=TAGTTTTGTCGGTGTCGGTG
PRIMER_LEFT_1=261,20
PRIMER_RIGHT_1=1245,20
PRIMER_LEFT_1_TM=58.378
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=985
PRIMER_PAIR_2_PENALTY=2.588655
PRIMER_LEFT_2_PENALTY=1.622186
PRIMER_RIGHT_2_PENALTY=0.966469
PRIMER_LEFT_2_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_2_SEQUENCE=GGTGTCGGTGACTTTTTGTG
PRIMER_LEFT_2=261,20
PRIMER_RIGHT_2=1235,20
PRIMER_LEFT_2_TM=58.378
PRIMER_RIGHT_2_TM=59.034
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=975
PRIMER_PAIR_3_PENALTY=2.606633
PRIMER_LEFT_3_PENALTY=1.622186
PRIMER_RIGHT_3_PENALTY=0.984448
PRIMER_LEFT_3_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_3_SEQUENCE=CGGTGTCGGTGACTTTTTGT
PRIMER_LEFT_3=261,20
PRIMER_RIGHT_3=1236,20
PRIMER_LEFT_3_TM=58.378
PRIMER_RIGHT_3_TM=60.984
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=976
PRIMER_PAIR_4_PENALTY=2.700003
PRIMER_LEFT_4_PENALTY=1.622186
PRIMER_RIGHT_4_PENALTY=1.077818
PRIMER_LEFT_4_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_4_SEQUENCE=GGTGTCGGTGACTTTTTGTGT
PRIMER_LEFT_4=261,20
PRIMER_RIGHT_4=1235,21
PRIMER_LEFT_4_TM=58.378
PRIMER_RIGHT_4_TM=59.922
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=975
PRIMER_PAIR_5_PENALTY=2.775774
PRIMER_LEFT_5_PENALTY=1.622186
PRIMER_RIGHT_5_PENALTY=1.153589
PRIMER_LEFT_5_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_5_SEQUENCE=TCTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_5=261,20
PRIMER_RIGHT_5=1248,21
PRIMER_LEFT_5_TM=58.378
PRIMER_RIGHT_5_TM=60.154
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=988
PRIMER_PAIR_6_PENALTY=3.174349
PRIMER_LEFT_6_PENALTY=1.622186
PRIMER_RIGHT_6_PENALTY=1.552164
PRIMER_LEFT_6_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_6_SEQUENCE=TGTCGGTGTCGGTGACTTTT
PRIMER_LEFT_6=261,20
PRIMER_RIGHT_6=1239,20
PRIMER_LEFT_6_TM=58.378
PRIMER_RIGHT_6_TM=61.552
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=979
PRIMER_PAIR_7_PENALTY=3.174349
PRIMER_LEFT_7_PENALTY=1.622186
PRIMER_RIGHT_7_PENALTY=1.552164
PRIMER_LEFT_7_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_7_SEQUENCE=TTGTCGGTGTCGGTGACTTT
PRIMER_LEFT_7=261,20
PRIMER_RIGHT_7=1240,20
PRIMER_LEFT_7_TM=58.378
PRIMER_RIGHT_7_TM=61.552
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=980
PRIMER_PAIR_8_PENALTY=3.174349
PRIMER_LEFT_8_PENALTY=1.622186
PRIMER_RIGHT_8_PENALTY=1.552164
PRIMER_LEFT_8_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_8_SEQUENCE=TTTGTCGGTGTCGGTGACTT
PRIMER_LEFT_8=261,20
PRIMER_RIGHT_8=1241,20
PRIMER_LEFT_8_TM=58.378
PRIMER_RIGHT_8_TM=61.552
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=981
PRIMER_PAIR_9_PENALTY=3.174349
PRIMER_LEFT_9_PENALTY=1.622186
PRIMER_RIGHT_9_PENALTY=1.552164
PRIMER_LEFT_9_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_9_SEQUENCE=TTTTGTCGGTGTCGGTGACT
PRIMER_LEFT_9=261,20
PRIMER_RIGHT_9=1242,20
PRIMER_LEFT_9_TM=58.378
PRIMER_RIGHT_9_TM=61.552
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=982
PRIMER_PAIR_10_PENALTY=3.174349
PRIMER_LEFT_10_PENALTY=1.622186
PRIMER_RIGHT_10_PENALTY=1.552164
PRIMER_LEFT_10_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_10_SEQUENCE=AGTTTTGTCGGTGTCGGTGA
PRIMER_LEFT_10=261,20
PRIMER_RIGHT_10=1244,20
PRIMER_LEFT_10_TM=58.378
PRIMER_RIGHT_10_TM=61.552
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=984
PRIMER_PAIR_11_PENALTY=3.339794
PRIMER_LEFT_11_PENALTY=1.622186
PRIMER_RIGHT_11_PENALTY=1.717608
PRIMER_LEFT_11_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_11_SEQUENCE=CTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_11=261,20
PRIMER_RIGHT_11=1247,20
PRIMER_LEFT_11_TM=58.378
PRIMER_RIGHT_11_TM=58.282
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=987
PRIMER_PAIR_12_PENALTY=3.466515
PRIMER_LEFT_12_PENALTY=1.622186
PRIMER_RIGHT_12_PENALTY=1.844329
PRIMER_LEFT_12_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_12_SEQUENCE=CTTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_12=261,20
PRIMER_RIGHT_12=1247,21
PRIMER_LEFT_12_TM=58.378
PRIMER_RIGHT_12_TM=59.156
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=987
PRIMER_PAIR_13_PENALTY=3.489987
PRIMER_LEFT_13_PENALTY=1.622186
PRIMER_RIGHT_13_PENALTY=1.867802
PRIMER_LEFT_13_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_13_SEQUENCE=TTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_13=261,20
PRIMER_RIGHT_13=1246,20
PRIMER_LEFT_13_TM=58.378
PRIMER_RIGHT_13_TM=58.132
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=986
PRIMER_PAIR_14_PENALTY=3.579773
PRIMER_LEFT_14_PENALTY=1.622186
PRIMER_RIGHT_14_PENALTY=1.957587
PRIMER_LEFT_14_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_14_SEQUENCE=TTAGTTTTGTCGGTGTCGGTG
PRIMER_LEFT_14=261,20
PRIMER_RIGHT_14=1246,21
PRIMER_LEFT_14_TM=58.378
PRIMER_RIGHT_14_TM=60.958
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=986
PRIMER_PAIR_15_PENALTY=3.679850
PRIMER_LEFT_15_PENALTY=1.622186
PRIMER_RIGHT_15_PENALTY=2.057665
PRIMER_LEFT_15_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_15_SEQUENCE=TCGGTGTCGGTGACTTTTTG
PRIMER_LEFT_15=261,20
PRIMER_RIGHT_15=1237,20
PRIMER_LEFT_15_TM=58.378
PRIMER_RIGHT_15_TM=62.058
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=977
PRIMER_PAIR_16_PENALTY=4.124582
PRIMER_LEFT_16_PENALTY=1.622186
PRIMER_RIGHT_16_PENALTY=2.502397
PRIMER_LEFT_16_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_16_SEQUENCE=TAGTTTTGTCGGTGTCGGTGA
PRIMER_LEFT_16=261,20
PRIMER_RIGHT_16=1245,21
PRIMER_LEFT_16_TM=58.378
PRIMER_RIGHT_16_TM=61.502
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=985
PRIMER_PAIR_17_PENALTY=4.346784
PRIMER_LEFT_17_PENALTY=1.622186
PRIMER_RIGHT_17_PENALTY=2.724598
PRIMER_LEFT_17_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_17_SEQUENCE=TCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_17=261,20
PRIMER_RIGHT_17=1231,20
PRIMER_LEFT_17_TM=58.378
PRIMER_RIGHT_17_TM=57.275
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=971
PRIMER_PAIR_18_PENALTY=4.364860
PRIMER_LEFT_18_PENALTY=1.622186
PRIMER_RIGHT_18_PENALTY=2.742674
PRIMER_LEFT_18_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_18_SEQUENCE=TCGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_18=261,20
PRIMER_RIGHT_18=1231,21
PRIMER_LEFT_18_TM=58.378
PRIMER_RIGHT_18_TM=58.257
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=971
PRIMER_PAIR_19_PENALTY=4.364860
PRIMER_LEFT_19_PENALTY=1.622186
PRIMER_RIGHT_19_PENALTY=2.742674
PRIMER_LEFT_19_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_19_SEQUENCE=GTCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_19=261,20
PRIMER_RIGHT_19=1232,21
PRIMER_LEFT_19_TM=58.378
PRIMER_RIGHT_19_TM=58.257
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=972
PRIMER_PAIR_20_PENALTY=4.461063
PRIMER_LEFT_20_PENALTY=1.622186
PRIMER_RIGHT_20_PENALTY=2.838878
PRIMER_LEFT_20_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_20_SEQUENCE=GTCGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_20=261,20
PRIMER_RIGHT_20=1232,22
PRIMER_LEFT_20_TM=58.378
PRIMER_RIGHT_20_TM=59.161
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=972
PRIMER_PAIR_21_PENALTY=4.517974
PRIMER_LEFT_21_PENALTY=1.622186
PRIMER_RIGHT_21_PENALTY=2.895789
PRIMER_LEFT_21_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_21_SEQUENCE=TGTCGGTGACTTTTTGTGTC
PRIMER_LEFT_21=261,20
PRIMER_RIGHT_21=1233,20
PRIMER_LEFT_21_TM=58.378
PRIMER_RIGHT_21_TM=57.104
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=6.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=973
PRIMER_PAIR_22_PENALTY=4.520597
PRIMER_LEFT_22_PENALTY=1.622186
PRIMER_RIGHT_22_PENALTY=2.898412
PRIMER_LEFT_22_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_22_SEQUENCE=GTGTCGGTGACTTTTTGTGTC
PRIMER_LEFT_22=261,20
PRIMER_RIGHT_22=1234,21
PRIMER_LEFT_22_TM=58.378
PRIMER_RIGHT_22_TM=58.102
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=974
PRIMER_PAIR_23_PENALTY=4.553685
PRIMER_LEFT_23_PENALTY=1.622186
PRIMER_RIGHT_23_PENALTY=2.931499
PRIMER_LEFT_23_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_23_SEQUENCE=TCTTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_23=261,20
PRIMER_RIGHT_23=1248,22
PRIMER_LEFT_23_TM=58.378
PRIMER_RIGHT_23_TM=60.931
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=4.553685
PRIMER_LEFT_24_PENALTY=1.622186
PRIMER_RIGHT_24_PENALTY=2.931499
PRIMER_LEFT_24_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_24_SEQUENCE=GTCTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_24=261,20
PRIMER_RIGHT_24=1249,22
PRIMER_LEFT_24_TM=58.378
PRIMER_RIGHT_24_TM=60.931
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=989
PRIMER_PAIR_25_PENALTY=4.697636
PRIMER_LEFT_25_PENALTY=1.622186
PRIMER_RIGHT_25_PENALTY=3.075450
PRIMER_LEFT_25_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_25_SEQUENCE=CCAAAGTCTTAGTTTTGTCGGTG
PRIMER_LEFT_25=261,20
PRIMER_RIGHT_25=1254,23
PRIMER_LEFT_25_TM=58.378
PRIMER_RIGHT_25_TM=60.075
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=994
PRIMER_PAIR_26_PENALTY=4.774664
PRIMER_LEFT_26_PENALTY=1.622186
PRIMER_RIGHT_26_PENALTY=3.152478
PRIMER_LEFT_26_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_26_SEQUENCE=TGTCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_26=261,20
PRIMER_RIGHT_26=1233,22
PRIMER_LEFT_26_TM=58.378
PRIMER_RIGHT_26_TM=61.152
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=973
PRIMER_PAIR_27_PENALTY=4.952976
PRIMER_LEFT_27_PENALTY=1.622186
PRIMER_RIGHT_27_PENALTY=3.330790
PRIMER_LEFT_27_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_27_SEQUENCE=GTTTTGTCGGTGTCGGTGACT
PRIMER_LEFT_27=261,20
PRIMER_RIGHT_27=1243,21
PRIMER_LEFT_27_TM=58.378
PRIMER_RIGHT_27_TM=62.331
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=983
PRIMER_PAIR_28_PENALTY=5.386985
PRIMER_LEFT_28_PENALTY=1.622186
PRIMER_RIGHT_28_PENALTY=3.764799
PRIMER_LEFT_28_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_28_SEQUENCE=GGTGTCGGTGACTTTTTGTGTC
PRIMER_LEFT_28=261,20
PRIMER_RIGHT_28=1235,22
PRIMER_LEFT_28_TM=58.378
PRIMER_RIGHT_28_TM=61.765
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=975
PRIMER_PAIR_29_PENALTY=5.426619
PRIMER_LEFT_29_PENALTY=1.622186
PRIMER_RIGHT_29_PENALTY=3.804433
PRIMER_LEFT_29_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_29_SEQUENCE=TCGGTGTCGGTGACTTTTTGT
PRIMER_LEFT_29=261,20
PRIMER_RIGHT_29=1237,21
PRIMER_LEFT_29_TM=58.378
PRIMER_RIGHT_29_TM=62.804
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=977
PRIMER_PAIR_30_PENALTY=5.426619
PRIMER_LEFT_30_PENALTY=1.622186
PRIMER_RIGHT_30_PENALTY=3.804433
PRIMER_LEFT_30_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_30_SEQUENCE=GTCGGTGTCGGTGACTTTTTG
PRIMER_LEFT_30=261,20
PRIMER_RIGHT_30=1238,21
PRIMER_LEFT_30_TM=58.378
PRIMER_RIGHT_30_TM=62.804
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=978
PRIMER_PAIR_31_PENALTY=5.426619
PRIMER_LEFT_31_PENALTY=1.622186
PRIMER_RIGHT_31_PENALTY=3.804433
PRIMER_LEFT_31_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_31_SEQUENCE=TGTCGGTGTCGGTGACTTTTT
PRIMER_LEFT_31=261,20
PRIMER_RIGHT_31=1239,21
PRIMER_LEFT_31_TM=58.378
PRIMER_RIGHT_31_TM=62.804
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=5.426619
PRIMER_LEFT_32_PENALTY=1.622186
PRIMER_RIGHT_32_PENALTY=3.804433
PRIMER_LEFT_32_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_32_SEQUENCE=TTGTCGGTGTCGGTGACTTTT
PRIMER_LEFT_32=261,20
PRIMER_RIGHT_32=1240,21
PRIMER_LEFT_32_TM=58.378
PRIMER_RIGHT_32_TM=62.804
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=980
PRIMER_PAIR_33_PENALTY=5.426619
PRIMER_LEFT_33_PENALTY=1.622186
PRIMER_RIGHT_33_PENALTY=3.804433
PRIMER_LEFT_33_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_33_SEQUENCE=TTTGTCGGTGTCGGTGACTTT
PRIMER_LEFT_33=261,20
PRIMER_RIGHT_33=1241,21
PRIMER_LEFT_33_TM=58.378
PRIMER_RIGHT_33_TM=62.804
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=981
PRIMER_PAIR_34_PENALTY=5.426619
PRIMER_LEFT_34_PENALTY=1.622186
PRIMER_RIGHT_34_PENALTY=3.804433
PRIMER_LEFT_34_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_34_SEQUENCE=TTTTGTCGGTGTCGGTGACTT
PRIMER_LEFT_34=261,20
PRIMER_RIGHT_34=1242,21
PRIMER_LEFT_34_TM=58.378
PRIMER_RIGHT_34_TM=62.804
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=982
PRIMER_PAIR_35_PENALTY=5.430007
PRIMER_LEFT_35_PENALTY=1.622186
PRIMER_RIGHT_35_PENALTY=3.807821
PRIMER_LEFT_35_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_35_SEQUENCE=CGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_35=261,20
PRIMER_RIGHT_35=1230,20
PRIMER_LEFT_35_TM=58.378
PRIMER_RIGHT_35_TM=56.192
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=970
PRIMER_PAIR_36_PENALTY=5.447618
PRIMER_LEFT_36_PENALTY=1.622186
PRIMER_RIGHT_36_PENALTY=3.825433
PRIMER_LEFT_36_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_36_SEQUENCE=CTTAGTTTTGTCGGTGTCGGTG
PRIMER_LEFT_36=261,20
PRIMER_RIGHT_36=1247,22
PRIMER_LEFT_36_TM=58.378
PRIMER_RIGHT_36_TM=61.825
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=9.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=987
PRIMER_PAIR_37_PENALTY=5.609038
PRIMER_LEFT_37_PENALTY=1.622186
PRIMER_RIGHT_37_PENALTY=3.986853
PRIMER_LEFT_37_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_37_SEQUENCE=GTGTCGGTGACTTTTTGTGT
PRIMER_LEFT_37=261,20
PRIMER_RIGHT_37=1234,20
PRIMER_LEFT_37_TM=58.378
PRIMER_RIGHT_37_TM=56.013
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=974
PRIMER_PAIR_38_PENALTY=6.130501
PRIMER_LEFT_38_PENALTY=1.622186
PRIMER_RIGHT_38_PENALTY=4.508316
PRIMER_LEFT_38_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_38_SEQUENCE=CCAAAGTCTTAGTTTTGTCGGT
PRIMER_LEFT_38=261,20
PRIMER_RIGHT_38=1254,22
PRIMER_LEFT_38_TM=58.378
PRIMER_RIGHT_38_TM=57.492
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=994
PRIMER_PAIR_39_PENALTY=6.275563
PRIMER_LEFT_39_PENALTY=1.622186
PRIMER_RIGHT_39_PENALTY=4.653377
PRIMER_LEFT_39_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_39_SEQUENCE=GTCTTAGTTTTGTCGGTGTCGGT
PRIMER_LEFT_39=261,20
PRIMER_RIGHT_39=1249,23
PRIMER_LEFT_39_TM=58.378
PRIMER_RIGHT_39_TM=61.653
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=47.826
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=989
PRIMER_PAIR_40_PENALTY=6.332979
PRIMER_LEFT_40_PENALTY=1.622186
PRIMER_RIGHT_40_PENALTY=4.710794
PRIMER_LEFT_40_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_40_SEQUENCE=TTAGTTTTGTCGGTGTCGGTGA
PRIMER_LEFT_40=261,20
PRIMER_RIGHT_40=1246,22
PRIMER_LEFT_40_TM=58.378
PRIMER_RIGHT_40_TM=62.711
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=986
PRIMER_PAIR_41_PENALTY=6.342591
PRIMER_LEFT_41_PENALTY=1.622186
PRIMER_RIGHT_41_PENALTY=4.720406
PRIMER_LEFT_41_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_41_SEQUENCE=CAAAGTCTTAGTTTTGTCGGTGTC
PRIMER_LEFT_41=261,20
PRIMER_RIGHT_41=1253,24
PRIMER_LEFT_41_TM=58.378
PRIMER_RIGHT_41_TM=59.280
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=41.667
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=6.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=993
PRIMER_PAIR_42_PENALTY=6.373775
PRIMER_LEFT_42_PENALTY=1.622186
PRIMER_RIGHT_42_PENALTY=4.751589
PRIMER_LEFT_42_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_42_SEQUENCE=CGGTGTCGGTGACTTTTTGTG
PRIMER_LEFT_42=261,20
PRIMER_RIGHT_42=1236,21
PRIMER_LEFT_42_TM=58.378
PRIMER_RIGHT_42_TM=63.752
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=976
PRIMER_PAIR_43_PENALTY=6.384269
PRIMER_LEFT_43_PENALTY=1.622186
PRIMER_RIGHT_43_PENALTY=4.762083
PRIMER_LEFT_43_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_43_SEQUENCE=AGTCTTAGTTTTGTCGGTGTCGG
PRIMER_LEFT_43=261,20
PRIMER_RIGHT_43=1250,23
PRIMER_LEFT_43_TM=58.378
PRIMER_RIGHT_43_TM=61.762
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=47.826
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=990
PRIMER_PAIR_44_PENALTY=6.415808
PRIMER_LEFT_44_PENALTY=1.622186
PRIMER_RIGHT_44_PENALTY=4.793622
PRIMER_LEFT_44_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_44_SEQUENCE=CCAAAGTCTTAGTTTTGTCGGTGT
PRIMER_LEFT_44=261,20
PRIMER_RIGHT_44=1254,24
PRIMER_LEFT_44_TM=58.378
PRIMER_RIGHT_44_TM=60.794
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=994
PRIMER_PAIR_45_PENALTY=6.415808
PRIMER_LEFT_45_PENALTY=1.622186
PRIMER_RIGHT_45_PENALTY=4.793622
PRIMER_LEFT_45_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_45_SEQUENCE=ACCAAAGTCTTAGTTTTGTCGGTG
PRIMER_LEFT_45=261,20
PRIMER_RIGHT_45=1255,24
PRIMER_LEFT_45_TM=58.378
PRIMER_RIGHT_45_TM=60.794
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=41.667
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=995
PRIMER_PAIR_46_PENALTY=6.540562
PRIMER_LEFT_46_PENALTY=1.622186
PRIMER_RIGHT_46_PENALTY=4.918376
PRIMER_LEFT_46_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_46_SEQUENCE=TGTCGGTGACTTTTTGTGTCAGT
PRIMER_LEFT_46=261,20
PRIMER_RIGHT_46=1233,23
PRIMER_LEFT_46_TM=58.378
PRIMER_RIGHT_46_TM=61.918
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=43.478
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=973
PRIMER_PAIR_47_PENALTY=6.540562
PRIMER_LEFT_47_PENALTY=1.622186
PRIMER_RIGHT_47_PENALTY=4.918376
PRIMER_LEFT_47_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_47_SEQUENCE=GTGTCGGTGACTTTTTGTGTCAG
PRIMER_LEFT_47=261,20
PRIMER_RIGHT_47=1234,23
PRIMER_LEFT_47_TM=58.378
PRIMER_RIGHT_47_TM=61.918
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=47.826
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=974
PRIMER_PAIR_48_PENALTY=6.777056
PRIMER_LEFT_48_PENALTY=1.622186
PRIMER_RIGHT_48_PENALTY=5.154871
PRIMER_LEFT_48_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_48_SEQUENCE=AGTTTTGTCGGTGTCGGTGACT
PRIMER_LEFT_48=261,20
PRIMER_RIGHT_48=1244,22
PRIMER_LEFT_48_TM=58.378
PRIMER_RIGHT_48_TM=63.155
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=984
PRIMER_PAIR_49_PENALTY=6.945041
PRIMER_LEFT_49_PENALTY=1.622186
PRIMER_RIGHT_49_PENALTY=5.322855
PRIMER_LEFT_49_SEQUENCE=CTCAAGAGGGTCGATGTCAG
PRIMER_RIGHT_49_SEQUENCE=CAAAGTCTTAGTTTTGTCGGTG
PRIMER_LEFT_49=261,20
PRIMER_RIGHT_49=1253,22
PRIMER_LEFT_49_TM=58.378
PRIMER_RIGHT_49_TM=56.677
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=9.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=993
=
SEQUENCE_ID=MAGIC.287_3_13891085_13891092_tsu-0_oy-0_275044_80691_TRUE
SEQUENCE_TARGET=950,107
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_13613039_13613105_oy-0_wil-2_13324_71471_TRUE
SEQUENCE_TARGET=950,166
SEQUENCE_TEMPLATE=CTAAAGGGCTACTGNNAAACCATGCATCACAAGAGAACGNTGCTNCAGTTTCTTTGTAATGTCANACTGNTTGCGNACNTTTTNCCCAACTTTTACCATCCTTAATGNCCTCTCAAATGAATGTCTACCCNACANGTAGCTCAAGAAGAAGTCNAAANTCTTAACAATCTCAACNATNNGGACTGATGGNNGGATANGTTANGTCCCACATATTNTAATACNTTCCANAATAACAATTAANACAAGTCGCNACCTANGCCACCCCAGGATCTGCCTATCAGATTTCAAAAGCTTGANAATATNCTTAATAGTGAAGTTCTTATGANGACTGAGCAACATTTCCAAATACATANAACCACTNCGCGGAGACGATTGGTTTTTACNTNTGGATTTCTATCAGGGAAAGNGNCANAGTAGAGACCTNTAACTTGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACCNGACTTNACNGCAACTTCTCNGCGACATAAGTGTTTGNGGAGTGAATGTGGAGTGCATAAGATTCAGGAAAGTGATANTAAAATCTANAAGTATAATACTAAATNTACNCANCTTCANACAAAGAAACATCTCTATCAACACTTTTGTCACCTTCACACTTCTCATCNACATTTCTATNAGTTAAACNGTCATTATATGANCTNANACNGTTGNTATGCTGCGTTGGGCCTGCAAATANAGACATTAANATGACAAACATTANCCCANAGACATGTTAAGCAGNCNNCAAAANTAGGCACATCATTCANTAAATGCTGGTTCTAACCGTCTGATGNTAAAATATATNTNTCANCCCTCANGGCAGCTTCACTCCNATCATCTCCNTTTCCAACAGAACCACCTTGACTAGATGAGGNCAGCGGGNTAGAGATGTTNGTTGTNGCTACGAGAAGGAACAAATAAATAGAAATATTGATATAATGCAAACAACGTGAANAGGAANCAACAATAAAGCANGGTTTATAACCTTCAGAAGAACGATGTCCATTTTCAGATATGNCANCTACANTAGATGAGGTAAGANTGGGATCACCATCAGACGGANCTGCAAGNACACACATCATATATAAAACTTTTCAAGCAAATTGTGTACATACNCCAAATTTGTCACACCTAACCTTCAGACGACACATCACTATCAACACTTATCTCATGTGAAACANAAACATCTCNGTTNCANTTGGAGTTACTGGAATGGATGAGGNCACAATAAANNAAACNTTAGTAACACAAGAAGAATATTTTCAATATCACGTTAGATTNTNAAGAACACCATTACGCATAACTAGTTAAATTAGCTGTTAGNATATGTAGGCAAATTAAAGTGGTAAGAAGTAACNNATTTGAAGGAATGGGGATGTAATTNAGTCCCACTAATAANAGGANATGCAATCANAAATACTAGANGCAACATAATTNTTGTGCCAAATTATAGANAAATCGCNATGTTTGNAAAATTGANAAACGNGAGATGAACAAAATTAGTAGTAGGANAANGATGTATCNNAGGCAATACCTGCAACTGAGATCTTCTGCGTCGNGATCCNAAGCANAATTTGCGAANTTCGCNGGATCAACAACTGTACAGCTGTGTCCCCCTTTCAAGTACTACGACGCCCTCGAGAAGGAGTAGAAGTTGGCNGAAGTTTCCAAGAGAAGTANGGTTTGACTCGCTTGACNGAGGGTGGGAAGGAACGCGAGTTNGAATTCNAACNCCNGTAACTCNTTGTTGTCACCATCNAAGTCGATTAAAANCNAGATCACCTGATNGAGGCCAGAAATAAGAACAATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.207372
PRIMER_LEFT_0_PENALTY=2.000037
PRIMER_RIGHT_0_PENALTY=2.207335
PRIMER_LEFT_0_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_0_SEQUENCE=TGTCGTCTGAAGGTTAGGTGTG
PRIMER_LEFT_0=752,22
PRIMER_RIGHT_0=1403,22
PRIMER_LEFT_0_TM=60.000
PRIMER_RIGHT_0_TM=60.207
PRIMER_LEFT_0_GC_PERCENT=45.455
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=652
PRIMER_PAIR_1_PENALTY=4.207372
PRIMER_LEFT_1_PENALTY=2.000037
PRIMER_RIGHT_1_PENALTY=2.207335
PRIMER_LEFT_1_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_1_SEQUENCE=TGTGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_1=752,22
PRIMER_RIGHT_1=1405,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.207
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=654
PRIMER_PAIR_2_PENALTY=4.305616
PRIMER_LEFT_2_PENALTY=2.132262
PRIMER_RIGHT_2_PENALTY=2.173354
PRIMER_LEFT_2_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_2_SEQUENCE=CCTCATCCATTCCAGTAACTCC
PRIMER_LEFT_2=760,22
PRIMER_RIGHT_2=1477,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=59.827
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=718
PRIMER_PAIR_3_PENALTY=4.339596
PRIMER_LEFT_3_PENALTY=2.132262
PRIMER_RIGHT_3_PENALTY=2.207335
PRIMER_LEFT_3_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_3_SEQUENCE=TGTCGTCTGAAGGTTAGGTGTG
PRIMER_LEFT_3=760,22
PRIMER_RIGHT_3=1403,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=60.207
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=644
PRIMER_PAIR_4_PENALTY=4.339596
PRIMER_LEFT_4_PENALTY=2.132262
PRIMER_RIGHT_4_PENALTY=2.207335
PRIMER_LEFT_4_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_4_SEQUENCE=TGTGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_4=760,22
PRIMER_RIGHT_4=1405,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=60.207
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=646
PRIMER_PAIR_5_PENALTY=4.643943
PRIMER_LEFT_5_PENALTY=2.000037
PRIMER_RIGHT_5_PENALTY=2.643906
PRIMER_LEFT_5_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_5_SEQUENCE=TCGTCTGAAGGTTAGGTGTGA
PRIMER_LEFT_5=752,22
PRIMER_RIGHT_5=1401,21
PRIMER_LEFT_5_TM=60.000
PRIMER_RIGHT_5_TM=58.356
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=650
PRIMER_PAIR_6_PENALTY=4.743248
PRIMER_LEFT_6_PENALTY=2.000037
PRIMER_RIGHT_6_PENALTY=2.743211
PRIMER_LEFT_6_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_6_SEQUENCE=GTCGTCTGAAGGTTAGGTGTGA
PRIMER_LEFT_6=752,22
PRIMER_RIGHT_6=1402,22
PRIMER_LEFT_6_TM=60.000
PRIMER_RIGHT_6_TM=59.257
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.2000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=651
PRIMER_PAIR_7_PENALTY=4.751744
PRIMER_LEFT_7_PENALTY=3.602821
PRIMER_RIGHT_7_PENALTY=1.148923
PRIMER_LEFT_7_SEQUENCE=TTTGTCACCTTCACACTTCTCA
PRIMER_RIGHT_7_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_7=857,22
PRIMER_RIGHT_7=1809,21
PRIMER_LEFT_7_TM=58.397
PRIMER_RIGHT_7_TM=60.149
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=953
PRIMER_PAIR_8_PENALTY=4.776168
PRIMER_LEFT_8_PENALTY=2.132262
PRIMER_RIGHT_8_PENALTY=2.643906
PRIMER_LEFT_8_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_8_SEQUENCE=TCGTCTGAAGGTTAGGTGTGA
PRIMER_LEFT_8=760,22
PRIMER_RIGHT_8=1401,21
PRIMER_LEFT_8_TM=60.132
PRIMER_RIGHT_8_TM=58.356
PRIMER_LEFT_8_GC_PERCENT=45.455
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=642
PRIMER_PAIR_9_PENALTY=4.875473
PRIMER_LEFT_9_PENALTY=2.132262
PRIMER_RIGHT_9_PENALTY=2.743211
PRIMER_LEFT_9_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_9_SEQUENCE=GTCGTCTGAAGGTTAGGTGTGA
PRIMER_LEFT_9=760,22
PRIMER_RIGHT_9=1402,22
PRIMER_LEFT_9_TM=60.132
PRIMER_RIGHT_9_TM=59.257
PRIMER_LEFT_9_GC_PERCENT=45.455
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=643
PRIMER_PAIR_10_PENALTY=4.902282
PRIMER_LEFT_10_PENALTY=3.753359
PRIMER_RIGHT_10_PENALTY=1.148923
PRIMER_LEFT_10_SEQUENCE=TTGTCACCTTCACACTTCTCATC
PRIMER_RIGHT_10_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_10=858,23
PRIMER_RIGHT_10=1809,21
PRIMER_LEFT_10_TM=59.247
PRIMER_RIGHT_10_TM=60.149
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=952
PRIMER_PAIR_11_PENALTY=5.149151
PRIMER_LEFT_11_PENALTY=1.844625
PRIMER_RIGHT_11_PENALTY=3.304526
PRIMER_LEFT_11_SEQUENCE=GCGGAGACGATTGGTTTTTAC
PRIMER_RIGHT_11_SEQUENCE=GAAAATGGACATCGTTCTTCTG
PRIMER_LEFT_11=362,21
PRIMER_RIGHT_11=1265,22
PRIMER_LEFT_11_TM=60.845
PRIMER_RIGHT_11_TM=58.695
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.0000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=904
PRIMER_PAIR_12_PENALTY=5.279289
PRIMER_LEFT_12_PENALTY=2.000037
PRIMER_RIGHT_12_PENALTY=3.279252
PRIMER_LEFT_12_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_12_SEQUENCE=GATGTGTCGTCTGAAGGTTAGG
PRIMER_LEFT_12=752,22
PRIMER_RIGHT_12=1407,22
PRIMER_LEFT_12_TM=60.000
PRIMER_RIGHT_12_TM=58.721
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.2000
PRIMER_RIGHT_12_END_STABILITY=7.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=656
PRIMER_PAIR_13_PENALTY=5.304563
PRIMER_LEFT_13_PENALTY=2.000037
PRIMER_RIGHT_13_PENALTY=3.304526
PRIMER_LEFT_13_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_13_SEQUENCE=GAAAATGGACATCGTTCTTCTG
PRIMER_LEFT_13=752,22
PRIMER_RIGHT_13=1265,22
PRIMER_LEFT_13_TM=60.000
PRIMER_RIGHT_13_TM=58.695
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.2000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=514
PRIMER_PAIR_14_PENALTY=5.330220
PRIMER_LEFT_14_PENALTY=4.181296
PRIMER_RIGHT_14_PENALTY=1.148923
PRIMER_LEFT_14_SEQUENCE=TTGTCACCTTCACACTTCTCA
PRIMER_RIGHT_14_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_14=858,21
PRIMER_RIGHT_14=1809,21
PRIMER_LEFT_14_TM=56.819
PRIMER_RIGHT_14_TM=60.149
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=952
PRIMER_PAIR_15_PENALTY=5.343686
PRIMER_LEFT_15_PENALTY=4.194762
PRIMER_RIGHT_15_PENALTY=1.148923
PRIMER_LEFT_15_SEQUENCE=CACTTTTGTCACCTTCACACTTC
PRIMER_RIGHT_15_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_15=853,23
PRIMER_RIGHT_15=1809,21
PRIMER_LEFT_15_TM=58.805
PRIMER_RIGHT_15_TM=60.149
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=957
PRIMER_PAIR_16_PENALTY=5.350062
PRIMER_LEFT_16_PENALTY=2.132262
PRIMER_RIGHT_16_PENALTY=3.217800
PRIMER_LEFT_16_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_16_SEQUENCE=ATTACATCCCCATTCCTTCA
PRIMER_LEFT_16=760,22
PRIMER_RIGHT_16=1637,20
PRIMER_LEFT_16_TM=60.132
PRIMER_RIGHT_16_TM=56.782
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=878
PRIMER_PAIR_17_PENALTY=5.379211
PRIMER_LEFT_17_PENALTY=4.230287
PRIMER_RIGHT_17_PENALTY=1.148923
PRIMER_LEFT_17_SEQUENCE=TGTCACCTTCACACTTCTCATC
PRIMER_RIGHT_17_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_17=859,22
PRIMER_RIGHT_17=1809,21
PRIMER_LEFT_17_TM=57.770
PRIMER_RIGHT_17_TM=60.149
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=951
PRIMER_PAIR_18_PENALTY=5.389279
PRIMER_LEFT_18_PENALTY=4.240356
PRIMER_RIGHT_18_PENALTY=1.148923
PRIMER_LEFT_18_SEQUENCE=TCAACACTTTTGTCACCTTCAC
PRIMER_RIGHT_18_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_18=849,22
PRIMER_RIGHT_18=1809,21
PRIMER_LEFT_18_TM=57.760
PRIMER_RIGHT_18_TM=60.149
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=961
PRIMER_PAIR_19_PENALTY=5.390983
PRIMER_LEFT_19_PENALTY=4.242059
PRIMER_RIGHT_19_PENALTY=1.148923
PRIMER_LEFT_19_SEQUENCE=CACTTTTGTCACCTTCACACTTCT
PRIMER_RIGHT_19_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_19=853,24
PRIMER_RIGHT_19=1809,21
PRIMER_LEFT_19_TM=59.758
PRIMER_RIGHT_19_TM=60.149
PRIMER_LEFT_19_GC_PERCENT=41.667
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=957
PRIMER_PAIR_20_PENALTY=5.436787
PRIMER_LEFT_20_PENALTY=2.132262
PRIMER_RIGHT_20_PENALTY=3.304526
PRIMER_LEFT_20_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_20_SEQUENCE=GAAAATGGACATCGTTCTTCTG
PRIMER_LEFT_20=760,22
PRIMER_RIGHT_20=1265,22
PRIMER_LEFT_20_TM=60.132
PRIMER_RIGHT_20_TM=58.695
PRIMER_LEFT_20_GC_PERCENT=45.455
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=506
PRIMER_PAIR_21_PENALTY=5.439322
PRIMER_LEFT_21_PENALTY=2.000037
PRIMER_RIGHT_21_PENALTY=3.439285
PRIMER_LEFT_21_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_21_SEQUENCE=GATGTGTCGTCTGAAGGTTAGGT
PRIMER_LEFT_21=752,22
PRIMER_RIGHT_21=1407,23
PRIMER_LEFT_21_TM=60.000
PRIMER_RIGHT_21_TM=59.561
PRIMER_LEFT_21_GC_PERCENT=45.455
PRIMER_RIGHT_21_GC_PERCENT=47.826
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.2000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=656
PRIMER_PAIR_22_PENALTY=5.462067
PRIMER_LEFT_22_PENALTY=2.000037
PRIMER_RIGHT_22_PENALTY=3.462030
PRIMER_LEFT_22_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_22_SEQUENCE=ATGTGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_22=752,22
PRIMER_RIGHT_22=1406,23
PRIMER_LEFT_22_TM=60.000
PRIMER_RIGHT_22_TM=60.462
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=47.826
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=655
PRIMER_PAIR_23_PENALTY=5.518430
PRIMER_LEFT_23_PENALTY=4.369507
PRIMER_RIGHT_23_PENALTY=1.148923
PRIMER_LEFT_23_SEQUENCE=ACACTTTTGTCACCTTCACACTTC
PRIMER_RIGHT_23_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_23=852,24
PRIMER_RIGHT_23=1809,21
PRIMER_LEFT_23_TM=59.630
PRIMER_RIGHT_23_TM=60.149
PRIMER_LEFT_23_GC_PERCENT=41.667
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=958
PRIMER_PAIR_24_PENALTY=5.559953
PRIMER_LEFT_24_PENALTY=3.386599
PRIMER_RIGHT_24_PENALTY=2.173354
PRIMER_LEFT_24_SEQUENCE=ATGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_24_SEQUENCE=CCTCATCCATTCCAGTAACTCC
PRIMER_LEFT_24=759,23
PRIMER_RIGHT_24=1477,22
PRIMER_LEFT_24_TM=60.387
PRIMER_RIGHT_24_TM=59.827
PRIMER_LEFT_24_GC_PERCENT=43.478
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=7.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=719
PRIMER_PAIR_25_PENALTY=5.579669
PRIMER_LEFT_25_PENALTY=3.406316
PRIMER_RIGHT_25_PENALTY=2.173354
PRIMER_LEFT_25_SEQUENCE=CAACACTTTTGTCACCTTCACAC
PRIMER_RIGHT_25_SEQUENCE=CCTCATCCATTCCAGTAACTCC
PRIMER_LEFT_25=850,23
PRIMER_RIGHT_25=1477,22
PRIMER_LEFT_25_TM=59.594
PRIMER_RIGHT_25_TM=59.827
PRIMER_LEFT_25_GC_PERCENT=43.478
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=628
PRIMER_PAIR_26_PENALTY=5.593933
PRIMER_LEFT_26_PENALTY=3.386599
PRIMER_RIGHT_26_PENALTY=2.207335
PRIMER_LEFT_26_SEQUENCE=ATGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_26_SEQUENCE=TGTCGTCTGAAGGTTAGGTGTG
PRIMER_LEFT_26=759,23
PRIMER_RIGHT_26=1403,22
PRIMER_LEFT_26_TM=60.387
PRIMER_RIGHT_26_TM=60.207
PRIMER_LEFT_26_GC_PERCENT=43.478
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=645
PRIMER_PAIR_27_PENALTY=5.593933
PRIMER_LEFT_27_PENALTY=3.386599
PRIMER_RIGHT_27_PENALTY=2.207335
PRIMER_LEFT_27_SEQUENCE=ATGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_27_SEQUENCE=TGTGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_27=759,23
PRIMER_RIGHT_27=1405,22
PRIMER_LEFT_27_TM=60.387
PRIMER_RIGHT_27_TM=60.207
PRIMER_LEFT_27_GC_PERCENT=43.478
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=647
PRIMER_PAIR_28_PENALTY=5.594292
PRIMER_LEFT_28_PENALTY=2.132262
PRIMER_RIGHT_28_PENALTY=3.462030
PRIMER_LEFT_28_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_28_SEQUENCE=ATGTGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_28=760,22
PRIMER_RIGHT_28=1406,23
PRIMER_LEFT_28_TM=60.132
PRIMER_RIGHT_28_TM=60.462
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=47.826
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=647
PRIMER_PAIR_29_PENALTY=5.693434
PRIMER_LEFT_29_PENALTY=2.000037
PRIMER_RIGHT_29_PENALTY=3.693397
PRIMER_LEFT_29_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_29_SEQUENCE=GTCGTCTGAAGGTTAGGTGTG
PRIMER_LEFT_29=752,22
PRIMER_RIGHT_29=1402,21
PRIMER_LEFT_29_TM=60.000
PRIMER_RIGHT_29_TM=57.307
PRIMER_LEFT_29_GC_PERCENT=45.455
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.2000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=651
PRIMER_PAIR_30_PENALTY=5.693434
PRIMER_LEFT_30_PENALTY=2.000037
PRIMER_RIGHT_30_PENALTY=3.693397
PRIMER_LEFT_30_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_30_SEQUENCE=TGTCGTCTGAAGGTTAGGTGT
PRIMER_LEFT_30=752,22
PRIMER_RIGHT_30=1403,21
PRIMER_LEFT_30_TM=60.000
PRIMER_RIGHT_30_TM=57.307
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.2000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=652
PRIMER_PAIR_31_PENALTY=5.693434
PRIMER_LEFT_31_PENALTY=2.000037
PRIMER_RIGHT_31_PENALTY=3.693397
PRIMER_LEFT_31_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_31_SEQUENCE=GTGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_31=752,22
PRIMER_RIGHT_31=1404,21
PRIMER_LEFT_31_TM=60.000
PRIMER_RIGHT_31_TM=57.307
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.2000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=653
PRIMER_PAIR_32_PENALTY=5.693434
PRIMER_LEFT_32_PENALTY=2.000037
PRIMER_RIGHT_32_PENALTY=3.693397
PRIMER_LEFT_32_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_32_SEQUENCE=TGTGTCGTCTGAAGGTTAGGT
PRIMER_LEFT_32=752,22
PRIMER_RIGHT_32=1405,21
PRIMER_LEFT_32_TM=60.000
PRIMER_RIGHT_32_TM=57.307
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.2000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=654
PRIMER_PAIR_33_PENALTY=5.707834
PRIMER_LEFT_33_PENALTY=2.000037
PRIMER_RIGHT_33_PENALTY=3.707797
PRIMER_LEFT_33_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_33_SEQUENCE=CGTCTGAAGGTTAGGTGTGA
PRIMER_LEFT_33=752,22
PRIMER_RIGHT_33=1400,20
PRIMER_LEFT_33_TM=60.000
PRIMER_RIGHT_33_TM=56.292
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.2000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=649
PRIMER_PAIR_34_PENALTY=5.707834
PRIMER_LEFT_34_PENALTY=2.000037
PRIMER_RIGHT_34_PENALTY=3.707797
PRIMER_LEFT_34_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_34_SEQUENCE=TCGTCTGAAGGTTAGGTGTG
PRIMER_LEFT_34=752,22
PRIMER_RIGHT_34=1401,20
PRIMER_LEFT_34_TM=60.000
PRIMER_RIGHT_34_TM=56.292
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.2000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=650
PRIMER_PAIR_35_PENALTY=5.707834
PRIMER_LEFT_35_PENALTY=2.000037
PRIMER_RIGHT_35_PENALTY=3.707797
PRIMER_LEFT_35_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_35_SEQUENCE=TGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_35=752,22
PRIMER_RIGHT_35=1403,20
PRIMER_LEFT_35_TM=60.000
PRIMER_RIGHT_35_TM=56.292
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.2000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=652
PRIMER_PAIR_36_PENALTY=5.707834
PRIMER_LEFT_36_PENALTY=2.000037
PRIMER_RIGHT_36_PENALTY=3.707797
PRIMER_LEFT_36_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_36_SEQUENCE=TGTGTCGTCTGAAGGTTAGG
PRIMER_LEFT_36=752,22
PRIMER_RIGHT_36=1405,20
PRIMER_LEFT_36_TM=60.000
PRIMER_RIGHT_36_TM=56.292
PRIMER_LEFT_36_GC_PERCENT=45.455
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.2000
PRIMER_RIGHT_36_END_STABILITY=7.5000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=654
PRIMER_PAIR_37_PENALTY=5.717387
PRIMER_LEFT_37_PENALTY=4.568464
PRIMER_RIGHT_37_PENALTY=1.148923
PRIMER_LEFT_37_SEQUENCE=TTTGTCACCTTCACACTTCTCATC
PRIMER_RIGHT_37_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_37=857,24
PRIMER_RIGHT_37=1809,21
PRIMER_LEFT_37_TM=60.568
PRIMER_RIGHT_37_TM=60.149
PRIMER_LEFT_37_GC_PERCENT=41.667
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=953
PRIMER_PAIR_38_PENALTY=5.759120
PRIMER_LEFT_38_PENALTY=2.000037
PRIMER_RIGHT_38_PENALTY=3.759083
PRIMER_LEFT_38_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_38_SEQUENCE=GTGTCGTCTGAAGGTTAGGTGT
PRIMER_LEFT_38=752,22
PRIMER_RIGHT_38=1404,22
PRIMER_LEFT_38_TM=60.000
PRIMER_RIGHT_38_TM=58.241
PRIMER_LEFT_38_GC_PERCENT=45.455
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.2000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=653
PRIMER_PAIR_39_PENALTY=5.776174
PRIMER_LEFT_39_PENALTY=3.602821
PRIMER_RIGHT_39_PENALTY=2.173354
PRIMER_LEFT_39_SEQUENCE=TTTGTCACCTTCACACTTCTCA
PRIMER_RIGHT_39_SEQUENCE=CCTCATCCATTCCAGTAACTCC
PRIMER_LEFT_39=857,22
PRIMER_RIGHT_39=1477,22
PRIMER_LEFT_39_TM=58.397
PRIMER_RIGHT_39_TM=59.827
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=621
PRIMER_PAIR_40_PENALTY=5.804085
PRIMER_LEFT_40_PENALTY=2.000037
PRIMER_RIGHT_40_PENALTY=3.804048
PRIMER_LEFT_40_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_40_SEQUENCE=AGTGATGTGTCGTCTGAAGGT
PRIMER_LEFT_40=752,22
PRIMER_RIGHT_40=1410,21
PRIMER_LEFT_40_TM=60.000
PRIMER_RIGHT_40_TM=57.196
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.2000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=659
PRIMER_PAIR_41_PENALTY=5.825659
PRIMER_LEFT_41_PENALTY=2.132262
PRIMER_RIGHT_41_PENALTY=3.693397
PRIMER_LEFT_41_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_41_SEQUENCE=GTCGTCTGAAGGTTAGGTGTG
PRIMER_LEFT_41=760,22
PRIMER_RIGHT_41=1402,21
PRIMER_LEFT_41_TM=60.132
PRIMER_RIGHT_41_TM=57.307
PRIMER_LEFT_41_GC_PERCENT=45.455
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=643
PRIMER_PAIR_42_PENALTY=5.825659
PRIMER_LEFT_42_PENALTY=2.132262
PRIMER_RIGHT_42_PENALTY=3.693397
PRIMER_LEFT_42_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_42_SEQUENCE=TGTCGTCTGAAGGTTAGGTGT
PRIMER_LEFT_42=760,22
PRIMER_RIGHT_42=1403,21
PRIMER_LEFT_42_TM=60.132
PRIMER_RIGHT_42_TM=57.307
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=644
PRIMER_PAIR_43_PENALTY=5.825659
PRIMER_LEFT_43_PENALTY=2.132262
PRIMER_RIGHT_43_PENALTY=3.693397
PRIMER_LEFT_43_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_43_SEQUENCE=GTGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_43=760,22
PRIMER_RIGHT_43=1404,21
PRIMER_LEFT_43_TM=60.132
PRIMER_RIGHT_43_TM=57.307
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=645
PRIMER_PAIR_44_PENALTY=5.840058
PRIMER_LEFT_44_PENALTY=2.132262
PRIMER_RIGHT_44_PENALTY=3.707797
PRIMER_LEFT_44_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_44_SEQUENCE=CGTCTGAAGGTTAGGTGTGA
PRIMER_LEFT_44=760,22
PRIMER_RIGHT_44=1400,20
PRIMER_LEFT_44_TM=60.132
PRIMER_RIGHT_44_TM=56.292
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=641
PRIMER_PAIR_45_PENALTY=5.840058
PRIMER_LEFT_45_PENALTY=2.132262
PRIMER_RIGHT_45_PENALTY=3.707797
PRIMER_LEFT_45_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_45_SEQUENCE=TCGTCTGAAGGTTAGGTGTG
PRIMER_LEFT_45=760,22
PRIMER_RIGHT_45=1401,20
PRIMER_LEFT_45_TM=60.132
PRIMER_RIGHT_45_TM=56.292
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=642
PRIMER_PAIR_46_PENALTY=5.840058
PRIMER_LEFT_46_PENALTY=2.132262
PRIMER_RIGHT_46_PENALTY=3.707797
PRIMER_LEFT_46_SEQUENCE=TGTGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_46_SEQUENCE=TGTCGTCTGAAGGTTAGGTG
PRIMER_LEFT_46=760,22
PRIMER_RIGHT_46=1403,20
PRIMER_LEFT_46_TM=60.132
PRIMER_RIGHT_46_TM=56.292
PRIMER_LEFT_46_GC_PERCENT=45.455
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=644
PRIMER_PAIR_47_PENALTY=5.868586
PRIMER_LEFT_47_PENALTY=2.000037
PRIMER_RIGHT_47_PENALTY=3.868549
PRIMER_LEFT_47_SEQUENCE=GGAGTGAATGTGGAGTGCATAA
PRIMER_RIGHT_47_SEQUENCE=AGTGATGTGTCGTCTGAAGG
PRIMER_LEFT_47=752,22
PRIMER_RIGHT_47=1410,20
PRIMER_LEFT_47_TM=60.000
PRIMER_RIGHT_47_TM=56.131
PRIMER_LEFT_47_GC_PERCENT=45.455
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.2000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=659
PRIMER_PAIR_48_PENALTY=5.869532
PRIMER_LEFT_48_PENALTY=4.720608
PRIMER_RIGHT_48_PENALTY=1.148923
PRIMER_LEFT_48_SEQUENCE=CTTTTGTCACCTTCACACTTCTCA
PRIMER_RIGHT_48_SEQUENCE=GCAGAAGATCTCAGTTGCAGG
PRIMER_LEFT_48=855,24
PRIMER_RIGHT_48=1809,21
PRIMER_LEFT_48_TM=60.721
PRIMER_RIGHT_48_TM=60.149
PRIMER_LEFT_48_GC_PERCENT=41.667
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=955
PRIMER_PAIR_49_PENALTY=5.888450
PRIMER_LEFT_49_PENALTY=3.715096
PRIMER_RIGHT_49_PENALTY=2.173354
PRIMER_LEFT_49_SEQUENCE=TGGAGTGCATAAGATTCAGG
PRIMER_RIGHT_49_SEQUENCE=CCTCATCCATTCCAGTAACTCC
PRIMER_LEFT_49=762,20
PRIMER_RIGHT_49=1477,22
PRIMER_LEFT_49_TM=56.285
PRIMER_RIGHT_49_TM=59.827
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=716
=
SEQUENCE_ID=MAGIC.287_3_18982843_18983120_wil-2_oy-0_5716_103201_TRUE
SEQUENCE_TARGET=950,377
SEQUENCE_TEMPLATE=TCGTGGCCAATTNTGCGATAAGATAAGATTCAATTACAATAACAGAGAGCTACACNGTGGATTTNAAAACCCTAATATAGAAGAAGCGGCCATTANAATCATCAATCAAAGGGGAAATTGGAGCTTTCGANGACTCNAAAAAGACGAATACCTTGACGGAGAGTACGAGGGATTCCGACGCAGCGAGGGTTCTTAGACTTGGATTTGAGAGTGGAAGAGTAATATAAATAGCCTTTGCACGATCTTCCTTTCTCCAGAGATCCTCTTTGTTNCTGTTCTGGTTTCTCTTCCATTACCGTCCTCGTTACTTCTTCGCTCGAGCCTTCGTTCATCGCGGAACTTGTCTATCTCTCTCTCTCCCTTTCGTTTCTTCTTCGAAATCTCTAGCTCTACCTTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTCATCATTTNTATTGGNTTTTTATTGGTTTAAAGCATGAATTNTNNAATTAATAATTTAATTTTTNAATTTTATGTTGATATTTANTTTAATNTTGAANTTTTGTTGANTAAGNCAATNAAAAAANATANTTAAAAATTTNNCAAATCATAAACTCTAGATCCAAAAACCNATTTATTTAATAAAAANTTTNNNTGANNTTTTNNNAAANNNNAAGTCTCNNNTAGCTTTGGAATAAGAGNAAAATAAAAATTAAGACAAAANTTTNATTTTACAATTNACTCACCTTTTAATTGATTTATTTATATANAAATAATTTCTNTTTAANANTGAAATCGANCGTNTTTTNTTTAATTCTTCTTCTTNNNNNNNNNNNNTTTTTTCAANATTTTCATCAATTTGATCTTNGTACACCTCCNTTTTAATTNTAAATTTCTCGGCGCAGAAAATTACNGGNAAATTTNACGGAGAAACCTCACCGANGAAGCAAAGTTATGACGGNCAAGGCTTTCCAATTTTTGTTTAGACTAAATCTTGTCTTTCCAATTTTTTCAGGTGTTGATCCCAAAAGCGAAACTAATTGGTTACACAAGAACTGATCCTTTCCATAAGGAATGTATAGAAANAGCAACAAGAATTGTTTGGTTTCCGATTCTGAGGTCANCTAAGTTAAATGAGCTTGCTAGTNTCTTGCCAGCAAGACTCGAGATTCAATGGTACAAAGACCGTTGTGATGCATCAGAAGAGCATGCAGCTAGGTTACTACGATTTCTTCAAACGATATTCGTTGAAGAGAGACTTNAAAGTGAACATNAGTCGCATAAGACTAGCTAAGTTTTGGGACAAAGTGATTAAAATGGTGGAGACGAATGAGTTACCTTCTGATTTTCANTTAGGAAAGAATCAAAGAAATGAATTTACGCATCTCAACATTCCTTATGGGAAAAAGAACTANTTCTGCAACAACTATAGTTCTCTTCTTATNTTGGGCCTTATACAAAAACACTTCAACAGTTTGAGCCTTGAACATAACCGATGGCCCAGTTGANAAAATATAAACCCATTTAGCAAATAAAAAAATGGAAAACATAATTTACAGAAAAAAAGAATGAGATAATATCAAATTGATTTNNNNNNNNGNAAAAAGGAAAATTAGCAGATTGGTAATTAAGTTTGNCTATAAATNATAATTAGAGAANAAAGAAAATTGAGTTGAAACGAAGTAATTAGAGNNNNAAGAAAGAGACGAAGAAGATGATGATGATTCCAAGGATGGTTAAGAACAAAACCCCTGCTCCGNTTCAAATCACGGCGGAGCAGGTTTTGAAGGANGCGAGAGAGAGAGAGGACAGTCGCATCCTGCGGCCGCCTAAACAGAAGATCACAGATTCGGATGAGNTTGCCNAGTACAGACTCCGACGTCGGAAGGAGTTTGAGGACNAGATCCGCGGCGCAAAAACGAACTCACAAGTTTGGGTGAGGTATGCTGATTGGGAAGAGTCTCAGAAGGATCACGATCGTGCTCGGAGCGTGTGGGAACGAGCTTTGGAAGACGAGNGTTNCAGGAACCATACGCTCTGGCTCAANTACGCAGAGTTCGAGATGAGGAACAAANNCGTCAACCATGCGAGGAACGTTTGGGATCGAGCCGTGAAGATTCTNC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_12388897_12389055_ct-1_hi-0_39811_8898_TRUE
SEQUENCE_TARGET=950,258
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNAGNAAAATAATTGAATATTTAATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATATTAGTAGCTAATAAATAAAANTTCAACATGATNTGNTCATATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGANTGNTAGANAAGTTCCCANANCTAGNTTGGAATGNNGANGGAGAATTGTCAACNCCATTGCATNATGCGTGCAATGCCAANAACCTCGAGATAACNAANATGNTNCTANANATTNATGAAAGCCTNGNAGAGAGGGTCAATANAGATGGTNTCACACNTTTGCATTTGGCGGCNNNNCATNNAATGCTCAATTCCAATTCTCANTGANTTTTCGGATAAGGCTCCANGATNTTTCNACATNCTAACACNAGNAAAAGNAACCGTCTTNCATCTAGCTGNGGAACANAAAAATNTANCANCGTTCTATNTCNTGGCANANAGTCCCGATAGAAACAATCTTNTNCACCAGNNNNNTAGATATGNCAACACNGTACTACANNNNNNNNNNNNNNNNNNNNNNNATTNCGTGAGTTTTTTTNTTNCTCTCTTAATTTCNGTNTGGAAGCTTAAAANACNTTTAGGTTTATTAAATAAATTGAGCGGAGTNGACTAAACTTACCAAAAACGAGAGTGCATTTTGGCACTTCNTANTTTTTTGGTAAGATTCGTTTGCTCNGTTCCATATGATCTTATTCTTATCANTNAAAGNANAGTAAATGATACATGNGANNNNNNNNNNNTTTTNTANTCATTCAAAGCAGATNTNNTTNATCATTNTATTTTTATAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNCAACAATAGATNTCNANGNCAAAAACACTCGAGGACTGGAAGCNNTTGATCTTNTCAANGTCNACNATGAAGATTATGGTAAGATATNCGGCTGNCTTAGNTTTGATGCAAAACAANTNCGAAATCCTANTNGTCCTCAACAANGAAANCATAACATTGNGAATNNNNNNGAATANANNNNNCNAATGTTTGAAACCTATGAAGCACCTCNAAGCAANGAAANNAACTTTAATNTNAATGTANNNNNNTTTTTTTTTAAGCTTGTTCGTAAGTGTAGCCNCTGACCGGCNAGTCGCTCGTNTNATGGATTNCAACTTTGTCAGAGTAGAAGTTTAGGTTTTCTATCTTTGGGAGGTANTATTGATTTGNTATANGAGGTTTCTCTAGTTNNGTTTAGTGATTGACTTTGATCATCTTAGGTTTTGAGAGTATTGTTGACATTNTTAGATCTTTAAAATCTTGGTTTAGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17776954_17777137_oy-0_ct-1_1694_6593_TRUE
SEQUENCE_TARGET=950,283
SEQUENCE_TEMPLATE=TCTCTATTCCTCCACGTGTCGCCCAATCATCGGCTTCCCATAACGTGGAATATACACCCATTGGTTGGAATCTTGGGTATGGTACTTTCCTTGCCTCATTGTTCTTGNAAACCCTGATGGGCACATTGTCTACGTAGAAGCTACACACCACACATATTTAGACTATTAGAAAGTGAATAACAGTTCTACATATATTGTTANTGCAAATAGAAATATCTTTGATCATTNNTTTTTTTNTTCTTATAAACTNATGGCNNNTGCCTAATNGATTATNAAAATGTCATTACNAAAAAAAATGGNTTATNAAANTGTGGGACATTTATAAGTTCAAATCATGAACGCCTCNAAAACAAATATGCTGTCTCTTTCATTCTCTTNTTACATGGCCCATGGGTCGACAAAATATGAAACCAATCATGTCTCTCGTGACAATAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAGACAAGAATAGTTAANACGAATCTATAGTACAAAAGTATTNGTTAATTTGNTANNGTTGATTCAANTATATGTCACATTCCTTTTAATAATNAGACAGAGANTGTGAAGAATAATGATAATTGGCTGTATATGTNNNNNNNNNNNNNNACANTANNNAATGTGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAATGTGACTTACACAATACGGAGATGGTTCCATGAGATGGCATATTCGTGGAANTCACGAGAAGGGTCGAACCAAAGGTTAACTCTTTGCTCTCTATCGCCTTTACCATGAGCAAACACATTGGTTTGCACTGTGTAAGGTTGTCCACTTCGATTTCCTANGAACTCAAAATCAAGCTCGTCNCGTACCGAATCGGTATCTGAATTCATCTGCGCAAATTAGTTTCATATTATAACCAAAACTTTTGAAATGCAGNGACATAAACTTGAAATATATGTATATATAGTTACGTAGAAGGCAGTGACAGTCCCGGCAGAATCACCGGGGATCAGTTTGATTTTCATGCTCACACGGCCGAACAAGTATTGCTTCTTCGAAGCAAATCCACATCCTAAACCAATGTTAATGAAAATAAATTAAGAATATGAATTGAAACTTNATANTATGTCTGAATGTAATTAAGTATAACGTATGTAAGAACCTGAGCTAGGGTCCAATTTGAGCTGAATGGCTCTGCCTCCGTCAATTTGAGTGATATGAGTATCGGACCATGCGATCCTAAAGTCATCTTCAAACTTAGCCGGTCTACTCATAACCGGCTGGTATAATGCTGCAAATAAGCATAAGCTTAACGTATAGAAAACATTTCTNGAAGAGAAGAGTGACACCACCATTGTTGCTTATGATGATGAGTTTGTTTTGGAAGTNGAAGATCGAAGGCATATTTATATNGAGATGCATGCAGGCAAATGTATGTACAATTATTGAGGAACNGTCTCATTTAGAGCTTAGATTTTCAGTACAGCTAATCACTGATCGATTTGTCACAATGATTTTGGACCTACCAATTTTCTATTATTGTTGTTATTATTATGTTTTTAAATTCACTGAATATGTCTTTGTTTTCTTTCCAAAATCTTACTGTTCTTNCACTTTTTTTTTAGAATTCAATTTATTCTGTGTACTTTTTCTCCTAGAACTATTCACATTATTATTGTGATAAAAGCTAAAATATTTTCTTGGCTCTCAGCCTCGCNATGATTGAAGCATAAGAATTTCCCAGTGTATAGCATAACTGAAAGGCGTGGTGCGGTTTTACTGGGACACGTGTCAGCATTACATTACTCTGCTATGTCTCGCAGATATGATACGATCGTCGTATCTTTCGTAGATTCTTAGATTTTATTATTTGTTGCCACACGATCGTTTCTTAGATTAATTTTTCCTTAAATACATGTTTATATTCGTTTGTTATATAGANNNNNNNNNNNNNNNNATATACAGTTTATCTTGTTCTCATTCGAGATGATAAATTTGTAATNNTTTTTTAATTTTCTTACATATTTTGGCATTTTGCTCCTTTTCCAATAAATTATACNTTATTGCTTTAATCAATGAGAGTATAGGTGCAGTATTTATGATTGTGCATGAACTTTCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.884662
PRIMER_LEFT_0_PENALTY=0.614325
PRIMER_RIGHT_0_PENALTY=0.270338
PRIMER_LEFT_0_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_0_SEQUENCE=CCACGCCTTTCAGTTATGCT
PRIMER_LEFT_0=883,20
PRIMER_RIGHT_0=1832,20
PRIMER_LEFT_0_TM=59.386
PRIMER_RIGHT_0_TM=60.270
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=950
PRIMER_PAIR_1_PENALTY=0.920474
PRIMER_LEFT_1_PENALTY=0.614325
PRIMER_RIGHT_1_PENALTY=0.306150
PRIMER_LEFT_1_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_1_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_1=883,20
PRIMER_RIGHT_1=1778,20
PRIMER_LEFT_1_TM=59.386
PRIMER_RIGHT_1_TM=59.694
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=896
PRIMER_PAIR_2_PENALTY=1.033010
PRIMER_LEFT_2_PENALTY=0.614325
PRIMER_RIGHT_2_PENALTY=0.418685
PRIMER_LEFT_2_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_2_SEQUENCE=GTGTCCCAGTAAAACCGCAC
PRIMER_LEFT_2=883,20
PRIMER_RIGHT_2=1851,20
PRIMER_LEFT_2_TM=59.386
PRIMER_RIGHT_2_TM=60.419
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=969
PRIMER_PAIR_3_PENALTY=1.294170
PRIMER_LEFT_3_PENALTY=0.988020
PRIMER_RIGHT_3_PENALTY=0.306150
PRIMER_LEFT_3_SEQUENCE=GAGAAGGGTCGAACCAAAGG
PRIMER_RIGHT_3_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_3=803,20
PRIMER_RIGHT_3=1778,20
PRIMER_LEFT_3_TM=60.988
PRIMER_RIGHT_3_TM=59.694
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=976
PRIMER_PAIR_4_PENALTY=1.317140
PRIMER_LEFT_4_PENALTY=1.010990
PRIMER_RIGHT_4_PENALTY=0.306150
PRIMER_LEFT_4_SEQUENCE=TGCTCTCTATCGCCTTTACCA
PRIMER_RIGHT_4_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_4=832,21
PRIMER_RIGHT_4=1778,20
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=59.694
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=947
PRIMER_PAIR_5_PENALTY=1.419422
PRIMER_LEFT_5_PENALTY=0.614325
PRIMER_RIGHT_5_PENALTY=0.805098
PRIMER_LEFT_5_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_5_SEQUENCE=CACCACGCCTTTCAGTTATG
PRIMER_LEFT_5=883,20
PRIMER_RIGHT_5=1834,20
PRIMER_LEFT_5_TM=59.386
PRIMER_RIGHT_5_TM=59.195
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=952
PRIMER_PAIR_6_PENALTY=1.625371
PRIMER_LEFT_6_PENALTY=1.355034
PRIMER_RIGHT_6_PENALTY=0.270338
PRIMER_LEFT_6_SEQUENCE=GGTTGTCCACTTCGATTTCCT
PRIMER_RIGHT_6_SEQUENCE=CCACGCCTTTCAGTTATGCT
PRIMER_LEFT_6=883,21
PRIMER_RIGHT_6=1832,20
PRIMER_LEFT_6_TM=60.355
PRIMER_RIGHT_6_TM=60.270
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=950
PRIMER_PAIR_7_PENALTY=1.625371
PRIMER_LEFT_7_PENALTY=1.355034
PRIMER_RIGHT_7_PENALTY=0.270338
PRIMER_LEFT_7_SEQUENCE=AGGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_7_SEQUENCE=CCACGCCTTTCAGTTATGCT
PRIMER_LEFT_7=882,21
PRIMER_RIGHT_7=1832,20
PRIMER_LEFT_7_TM=60.355
PRIMER_RIGHT_7_TM=60.270
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=951
PRIMER_PAIR_8_PENALTY=1.661183
PRIMER_LEFT_8_PENALTY=1.355034
PRIMER_RIGHT_8_PENALTY=0.306150
PRIMER_LEFT_8_SEQUENCE=AGGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_8_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_8=882,21
PRIMER_RIGHT_8=1778,20
PRIMER_LEFT_8_TM=60.355
PRIMER_RIGHT_8_TM=59.694
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=897
PRIMER_PAIR_9_PENALTY=1.773719
PRIMER_LEFT_9_PENALTY=1.355034
PRIMER_RIGHT_9_PENALTY=0.418685
PRIMER_LEFT_9_SEQUENCE=GGTTGTCCACTTCGATTTCCT
PRIMER_RIGHT_9_SEQUENCE=GTGTCCCAGTAAAACCGCAC
PRIMER_LEFT_9=883,21
PRIMER_RIGHT_9=1851,20
PRIMER_LEFT_9_TM=60.355
PRIMER_RIGHT_9_TM=60.419
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=9.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=969
PRIMER_PAIR_10_PENALTY=1.773719
PRIMER_LEFT_10_PENALTY=1.355034
PRIMER_RIGHT_10_PENALTY=0.418685
PRIMER_LEFT_10_SEQUENCE=AGGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_10_SEQUENCE=GTGTCCCAGTAAAACCGCAC
PRIMER_LEFT_10=882,21
PRIMER_RIGHT_10=1851,20
PRIMER_LEFT_10_TM=60.355
PRIMER_RIGHT_10_TM=60.419
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=9.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=970
PRIMER_PAIR_11_PENALTY=1.818061
PRIMER_LEFT_11_PENALTY=0.614325
PRIMER_RIGHT_11_PENALTY=1.203737
PRIMER_LEFT_11_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_11_SEQUENCE=TTTGCAGCATTATACCAGCC
PRIMER_LEFT_11=883,20
PRIMER_RIGHT_11=1361,20
PRIMER_LEFT_11_TM=59.386
PRIMER_RIGHT_11_TM=58.796
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=479
PRIMER_PAIR_12_PENALTY=2.057063
PRIMER_LEFT_12_PENALTY=0.614325
PRIMER_RIGHT_12_PENALTY=1.442739
PRIMER_LEFT_12_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_12_SEQUENCE=ATCGCATGGTCCGATACTCA
PRIMER_LEFT_12=883,20
PRIMER_RIGHT_12=1301,20
PRIMER_LEFT_12_TM=59.386
PRIMER_RIGHT_12_TM=61.443
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=419
PRIMER_PAIR_13_PENALTY=2.061176
PRIMER_LEFT_13_PENALTY=0.033245
PRIMER_RIGHT_13_PENALTY=2.027931
PRIMER_LEFT_13_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_13_SEQUENCE=CCGGCTAAGTTTGAAGATGA
PRIMER_LEFT_13=777,20
PRIMER_RIGHT_13=1328,20
PRIMER_LEFT_13_TM=60.033
PRIMER_RIGHT_13_TM=57.972
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=552
PRIMER_PAIR_14_PENALTY=2.160131
PRIMER_LEFT_14_PENALTY=1.355034
PRIMER_RIGHT_14_PENALTY=0.805098
PRIMER_LEFT_14_SEQUENCE=GGTTGTCCACTTCGATTTCCT
PRIMER_RIGHT_14_SEQUENCE=CACCACGCCTTTCAGTTATG
PRIMER_LEFT_14=883,21
PRIMER_RIGHT_14=1834,20
PRIMER_LEFT_14_TM=60.355
PRIMER_RIGHT_14_TM=59.195
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=952
PRIMER_PAIR_15_PENALTY=2.160131
PRIMER_LEFT_15_PENALTY=1.355034
PRIMER_RIGHT_15_PENALTY=0.805098
PRIMER_LEFT_15_SEQUENCE=AGGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_15_SEQUENCE=CACCACGCCTTTCAGTTATG
PRIMER_LEFT_15=882,21
PRIMER_RIGHT_15=1834,20
PRIMER_LEFT_15_TM=60.355
PRIMER_RIGHT_15_TM=59.195
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=953
PRIMER_PAIR_16_PENALTY=2.186078
PRIMER_LEFT_16_PENALTY=0.033245
PRIMER_RIGHT_16_PENALTY=2.152833
PRIMER_LEFT_16_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_16_SEQUENCE=CCGGCTAAGTTTGAAGATGAC
PRIMER_LEFT_16=777,20
PRIMER_RIGHT_16=1328,21
PRIMER_LEFT_16_TM=60.033
PRIMER_RIGHT_16_TM=58.847
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=552
PRIMER_PAIR_17_PENALTY=2.186078
PRIMER_LEFT_17_PENALTY=0.033245
PRIMER_RIGHT_17_PENALTY=2.152833
PRIMER_LEFT_17_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_17_SEQUENCE=ACCGGCTAAGTTTGAAGATGA
PRIMER_LEFT_17=777,20
PRIMER_RIGHT_17=1329,21
PRIMER_LEFT_17_TM=60.033
PRIMER_RIGHT_17_TM=58.847
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=553
PRIMER_PAIR_18_PENALTY=2.186078
PRIMER_LEFT_18_PENALTY=0.033245
PRIMER_RIGHT_18_PENALTY=2.152833
PRIMER_LEFT_18_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_18_SEQUENCE=GACCGGCTAAGTTTGAAGATG
PRIMER_LEFT_18=777,20
PRIMER_RIGHT_18=1330,21
PRIMER_LEFT_18_TM=60.033
PRIMER_RIGHT_18_TM=58.847
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=554
PRIMER_PAIR_19_PENALTY=2.191757
PRIMER_LEFT_19_PENALTY=0.988020
PRIMER_RIGHT_19_PENALTY=1.203737
PRIMER_LEFT_19_SEQUENCE=GAGAAGGGTCGAACCAAAGG
PRIMER_RIGHT_19_SEQUENCE=TTTGCAGCATTATACCAGCC
PRIMER_LEFT_19=803,20
PRIMER_RIGHT_19=1361,20
PRIMER_LEFT_19_TM=60.988
PRIMER_RIGHT_19_TM=58.796
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=559
PRIMER_PAIR_20_PENALTY=2.214727
PRIMER_LEFT_20_PENALTY=1.010990
PRIMER_RIGHT_20_PENALTY=1.203737
PRIMER_LEFT_20_SEQUENCE=TGCTCTCTATCGCCTTTACCA
PRIMER_RIGHT_20_SEQUENCE=TTTGCAGCATTATACCAGCC
PRIMER_LEFT_20=832,21
PRIMER_RIGHT_20=1361,20
PRIMER_LEFT_20_TM=59.989
PRIMER_RIGHT_20_TM=58.796
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.2000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=530
PRIMER_PAIR_21_PENALTY=2.247293
PRIMER_LEFT_21_PENALTY=1.941143
PRIMER_RIGHT_21_PENALTY=0.306150
PRIMER_LEFT_21_SEQUENCE=ACGAGAAGGGTCGAACCAAA
PRIMER_RIGHT_21_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_21=801,20
PRIMER_RIGHT_21=1778,20
PRIMER_LEFT_21_TM=61.941
PRIMER_RIGHT_21_TM=59.694
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.8000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=978
PRIMER_PAIR_22_PENALTY=2.249833
PRIMER_LEFT_22_PENALTY=0.033245
PRIMER_RIGHT_22_PENALTY=2.216588
PRIMER_LEFT_22_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_22_SEQUENCE=CCGGCTAAGTTTGAAGATGACT
PRIMER_LEFT_22=777,20
PRIMER_RIGHT_22=1328,22
PRIMER_LEFT_22_TM=60.033
PRIMER_RIGHT_22_TM=59.783
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=552
PRIMER_PAIR_23_PENALTY=2.249833
PRIMER_LEFT_23_PENALTY=0.033245
PRIMER_RIGHT_23_PENALTY=2.216588
PRIMER_LEFT_23_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_23_SEQUENCE=AGACCGGCTAAGTTTGAAGATG
PRIMER_LEFT_23=777,20
PRIMER_RIGHT_23=1331,22
PRIMER_LEFT_23_TM=60.033
PRIMER_RIGHT_23_TM=59.783
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=555
PRIMER_PAIR_24_PENALTY=2.324914
PRIMER_LEFT_24_PENALTY=0.033245
PRIMER_RIGHT_24_PENALTY=2.291669
PRIMER_LEFT_24_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_24_SEQUENCE=CATAAGCAACAATGGTGGTGTC
PRIMER_LEFT_24=777,20
PRIMER_RIGHT_24=1429,22
PRIMER_LEFT_24_TM=60.033
PRIMER_RIGHT_24_TM=60.292
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=653
PRIMER_PAIR_25_PENALTY=2.363947
PRIMER_LEFT_25_PENALTY=2.057797
PRIMER_RIGHT_25_PENALTY=0.306150
PRIMER_LEFT_25_SEQUENCE=CGAGAAGGGTCGAACCAAAG
PRIMER_RIGHT_25_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_25=802,20
PRIMER_RIGHT_25=1778,20
PRIMER_LEFT_25_TM=62.058
PRIMER_RIGHT_25_TM=59.694
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=7.3000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=977
PRIMER_PAIR_26_PENALTY=2.375121
PRIMER_LEFT_26_PENALTY=0.033245
PRIMER_RIGHT_26_PENALTY=2.341875
PRIMER_LEFT_26_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_26_SEQUENCE=ACCGGCTAAGTTTGAAGATGAC
PRIMER_LEFT_26=777,20
PRIMER_RIGHT_26=1329,22
PRIMER_LEFT_26_TM=60.033
PRIMER_RIGHT_26_TM=59.658
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.3000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=553
PRIMER_PAIR_27_PENALTY=2.411077
PRIMER_LEFT_27_PENALTY=0.033245
PRIMER_RIGHT_27_PENALTY=2.377832
PRIMER_LEFT_27_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_27_SEQUENCE=CAATGGTGGTGTCACTCTTCTC
PRIMER_LEFT_27=777,20
PRIMER_RIGHT_27=1420,22
PRIMER_LEFT_27_TM=60.033
PRIMER_RIGHT_27_TM=59.622
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=644
PRIMER_PAIR_28_PENALTY=2.430759
PRIMER_LEFT_28_PENALTY=0.988020
PRIMER_RIGHT_28_PENALTY=1.442739
PRIMER_LEFT_28_SEQUENCE=GAGAAGGGTCGAACCAAAGG
PRIMER_RIGHT_28_SEQUENCE=ATCGCATGGTCCGATACTCA
PRIMER_LEFT_28=803,20
PRIMER_RIGHT_28=1301,20
PRIMER_LEFT_28_TM=60.988
PRIMER_RIGHT_28_TM=61.443
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=499
PRIMER_PAIR_29_PENALTY=2.453729
PRIMER_LEFT_29_PENALTY=1.010990
PRIMER_RIGHT_29_PENALTY=1.442739
PRIMER_LEFT_29_SEQUENCE=TGCTCTCTATCGCCTTTACCA
PRIMER_RIGHT_29_SEQUENCE=ATCGCATGGTCCGATACTCA
PRIMER_LEFT_29=832,21
PRIMER_RIGHT_29=1301,20
PRIMER_LEFT_29_TM=59.989
PRIMER_RIGHT_29_TM=61.443
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.2000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=470
PRIMER_PAIR_30_PENALTY=2.497055
PRIMER_LEFT_30_PENALTY=0.614325
PRIMER_RIGHT_30_PENALTY=1.882731
PRIMER_LEFT_30_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_30_SEQUENCE=ATTTGCAGCATTATACCAGCC
PRIMER_LEFT_30=883,20
PRIMER_RIGHT_30=1362,21
PRIMER_LEFT_30_TM=59.386
PRIMER_RIGHT_30_TM=59.117
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=480
PRIMER_PAIR_31_PENALTY=2.555784
PRIMER_LEFT_31_PENALTY=0.033245
PRIMER_RIGHT_31_PENALTY=2.522539
PRIMER_LEFT_31_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_31_SEQUENCE=CAACAATGGTGGTGTCACTCT
PRIMER_LEFT_31=777,20
PRIMER_RIGHT_31=1423,21
PRIMER_LEFT_31_TM=60.033
PRIMER_RIGHT_31_TM=58.477
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=6.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=647
PRIMER_PAIR_32_PENALTY=2.558770
PRIMER_LEFT_32_PENALTY=1.355034
PRIMER_RIGHT_32_PENALTY=1.203737
PRIMER_LEFT_32_SEQUENCE=GGTTGTCCACTTCGATTTCCT
PRIMER_RIGHT_32_SEQUENCE=TTTGCAGCATTATACCAGCC
PRIMER_LEFT_32=883,21
PRIMER_RIGHT_32=1361,20
PRIMER_LEFT_32_TM=60.355
PRIMER_RIGHT_32_TM=58.796
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.7000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=479
PRIMER_PAIR_33_PENALTY=2.558770
PRIMER_LEFT_33_PENALTY=1.355034
PRIMER_RIGHT_33_PENALTY=1.203737
PRIMER_LEFT_33_SEQUENCE=AGGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_33_SEQUENCE=TTTGCAGCATTATACCAGCC
PRIMER_LEFT_33=882,21
PRIMER_RIGHT_33=1361,20
PRIMER_LEFT_33_TM=60.355
PRIMER_RIGHT_33_TM=58.796
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=480
PRIMER_PAIR_34_PENALTY=2.618814
PRIMER_LEFT_34_PENALTY=2.312665
PRIMER_RIGHT_34_PENALTY=0.306150
PRIMER_LEFT_34_SEQUENCE=CCTTTACCATGAGCAAACACA
PRIMER_RIGHT_34_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_34=844,21
PRIMER_RIGHT_34=1778,20
PRIMER_LEFT_34_TM=58.687
PRIMER_RIGHT_34_TM=59.694
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.3000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=935
PRIMER_PAIR_35_PENALTY=2.642255
PRIMER_LEFT_35_PENALTY=0.614325
PRIMER_RIGHT_35_PENALTY=2.027931
PRIMER_LEFT_35_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_35_SEQUENCE=CCGGCTAAGTTTGAAGATGA
PRIMER_LEFT_35=883,20
PRIMER_RIGHT_35=1328,20
PRIMER_LEFT_35_TM=59.386
PRIMER_RIGHT_35_TM=57.972
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=6.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=446
PRIMER_PAIR_36_PENALTY=2.645415
PRIMER_LEFT_36_PENALTY=0.033245
PRIMER_RIGHT_36_PENALTY=2.612170
PRIMER_LEFT_36_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_36_SEQUENCE=GACCGGCTAAGTTTGAAGATGA
PRIMER_LEFT_36=777,20
PRIMER_RIGHT_36=1330,22
PRIMER_LEFT_36_TM=60.033
PRIMER_RIGHT_36_TM=60.612
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=6.6000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=554
PRIMER_PAIR_37_PENALTY=2.662241
PRIMER_LEFT_37_PENALTY=0.614325
PRIMER_RIGHT_37_PENALTY=2.047917
PRIMER_LEFT_37_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_37_SEQUENCE=ACCACGCCTTTCAGTTATGCT
PRIMER_LEFT_37=883,20
PRIMER_RIGHT_37=1833,21
PRIMER_LEFT_37_TM=59.386
PRIMER_RIGHT_37_TM=61.048
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=8.1000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=951
PRIMER_PAIR_38_PENALTY=2.682910
PRIMER_LEFT_38_PENALTY=0.033245
PRIMER_RIGHT_38_PENALTY=2.649665
PRIMER_LEFT_38_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_38_SEQUENCE=TGGTGGTGTCACTCTTCTCTTC
PRIMER_LEFT_38=777,20
PRIMER_RIGHT_38=1417,22
PRIMER_LEFT_38_TM=60.033
PRIMER_RIGHT_38_TM=59.350
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=641
PRIMER_PAIR_39_PENALTY=2.688382
PRIMER_LEFT_39_PENALTY=2.382233
PRIMER_RIGHT_39_PENALTY=0.306150
PRIMER_LEFT_39_SEQUENCE=TTGCTCTCTATCGCCTTTACC
PRIMER_RIGHT_39_SEQUENCE=GAGGCTGAGAGCCAAGAAAA
PRIMER_LEFT_39=831,21
PRIMER_RIGHT_39=1778,20
PRIMER_LEFT_39_TM=58.618
PRIMER_RIGHT_39_TM=59.694
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=948
PRIMER_PAIR_40_PENALTY=2.735220
PRIMER_LEFT_40_PENALTY=0.033245
PRIMER_RIGHT_40_PENALTY=2.701974
PRIMER_LEFT_40_SEQUENCE=TGAGATGGCATATTCGTGGA
PRIMER_RIGHT_40_SEQUENCE=ACCAGCCGGTTATGAGTAGA
PRIMER_LEFT_40=777,20
PRIMER_RIGHT_40=1348,20
PRIMER_LEFT_40_TM=60.033
PRIMER_RIGHT_40_TM=57.298
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=5.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=572
PRIMER_PAIR_41_PENALTY=2.767157
PRIMER_LEFT_41_PENALTY=0.614325
PRIMER_RIGHT_41_PENALTY=2.152833
PRIMER_LEFT_41_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_41_SEQUENCE=CCGGCTAAGTTTGAAGATGAC
PRIMER_LEFT_41=883,20
PRIMER_RIGHT_41=1328,21
PRIMER_LEFT_41_TM=59.386
PRIMER_RIGHT_41_TM=58.847
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.3000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=446
PRIMER_PAIR_42_PENALTY=2.767157
PRIMER_LEFT_42_PENALTY=0.614325
PRIMER_RIGHT_42_PENALTY=2.152833
PRIMER_LEFT_42_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_42_SEQUENCE=ACCGGCTAAGTTTGAAGATGA
PRIMER_LEFT_42=883,20
PRIMER_RIGHT_42=1329,21
PRIMER_LEFT_42_TM=59.386
PRIMER_RIGHT_42_TM=58.847
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=447
PRIMER_PAIR_43_PENALTY=2.767157
PRIMER_LEFT_43_PENALTY=0.614325
PRIMER_RIGHT_43_PENALTY=2.152833
PRIMER_LEFT_43_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_43_SEQUENCE=GACCGGCTAAGTTTGAAGATG
PRIMER_LEFT_43=883,20
PRIMER_RIGHT_43=1330,21
PRIMER_LEFT_43_TM=59.386
PRIMER_RIGHT_43_TM=58.847
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=448
PRIMER_PAIR_44_PENALTY=2.774003
PRIMER_LEFT_44_PENALTY=1.570267
PRIMER_RIGHT_44_PENALTY=1.203737
PRIMER_LEFT_44_SEQUENCE=CCATGGGTCGACAAAATATGA
PRIMER_RIGHT_44_SEQUENCE=TTTGCAGCATTATACCAGCC
PRIMER_LEFT_44=387,21
PRIMER_RIGHT_44=1361,20
PRIMER_LEFT_44_TM=60.570
PRIMER_RIGHT_44_TM=58.796
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=5.9000
PRIMER_RIGHT_44_END_STABILITY=9.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=975
PRIMER_PAIR_45_PENALTY=2.797772
PRIMER_LEFT_45_PENALTY=1.355034
PRIMER_RIGHT_45_PENALTY=1.442739
PRIMER_LEFT_45_SEQUENCE=GGTTGTCCACTTCGATTTCCT
PRIMER_RIGHT_45_SEQUENCE=ATCGCATGGTCCGATACTCA
PRIMER_LEFT_45=883,21
PRIMER_RIGHT_45=1301,20
PRIMER_LEFT_45_TM=60.355
PRIMER_RIGHT_45_TM=61.443
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=419
PRIMER_PAIR_46_PENALTY=2.797772
PRIMER_LEFT_46_PENALTY=1.355034
PRIMER_RIGHT_46_PENALTY=1.442739
PRIMER_LEFT_46_SEQUENCE=AGGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_46_SEQUENCE=ATCGCATGGTCCGATACTCA
PRIMER_LEFT_46=882,21
PRIMER_RIGHT_46=1301,20
PRIMER_LEFT_46_TM=60.355
PRIMER_RIGHT_46_TM=61.443
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=420
PRIMER_PAIR_47_PENALTY=2.807377
PRIMER_LEFT_47_PENALTY=1.603640
PRIMER_RIGHT_47_PENALTY=1.203737
PRIMER_LEFT_47_SEQUENCE=ATGAGATGGCATATTCGTGG
PRIMER_RIGHT_47_SEQUENCE=TTTGCAGCATTATACCAGCC
PRIMER_LEFT_47=776,20
PRIMER_RIGHT_47=1361,20
PRIMER_LEFT_47_TM=58.396
PRIMER_RIGHT_47_TM=58.796
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=586
PRIMER_PAIR_48_PENALTY=2.830912
PRIMER_LEFT_48_PENALTY=0.614325
PRIMER_RIGHT_48_PENALTY=2.216588
PRIMER_LEFT_48_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_48_SEQUENCE=CCGGCTAAGTTTGAAGATGACT
PRIMER_LEFT_48=883,20
PRIMER_RIGHT_48=1328,22
PRIMER_LEFT_48_TM=59.386
PRIMER_RIGHT_48_TM=59.783
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=446
PRIMER_PAIR_49_PENALTY=2.830912
PRIMER_LEFT_49_PENALTY=0.614325
PRIMER_RIGHT_49_PENALTY=2.216588
PRIMER_LEFT_49_SEQUENCE=GGTTGTCCACTTCGATTTCC
PRIMER_RIGHT_49_SEQUENCE=AGACCGGCTAAGTTTGAAGATG
PRIMER_LEFT_49=883,20
PRIMER_RIGHT_49=1331,22
PRIMER_LEFT_49_TM=59.386
PRIMER_RIGHT_49_TM=59.783
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=6.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=449
=
SEQUENCE_ID=MAGIC.426_2_10468870_10469001_oy-0_rsch-4_815032_169912_TRUE
SEQUENCE_TARGET=950,231
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5339907_5339998_tsu-0_oy-0_8462_130616_TRUE
SEQUENCE_TARGET=950,191
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_8232276_8232712_sf-2_bur-0_350216_825369_TRUE
SEQUENCE_TARGET=950,536
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGTTATAGTTAAANAGATTATTTTATGGGCATTNNTNTTTTTTTNNTCNACAATATATATTGATTCCACTCAAAAGAGCCAAACTTGGAAATGCTGGTNTTTTGGAAAATGATATTANTAATGAAGTTTTTGGAAAAGAAAAGAAAAANACATTTTAGTGGTAATTCTCTTCTTTTCAATNTATTTTTGTTTTATGTTTTGATCTTCTCATATTTGTGNNNNNTCANCGGATTGTAAAGANGAANAACATNTCCAAANGNANCTCCATGATCATCGAGAAGAANTTAATGAACAAGACTACTCATGTGAAGCAAATNAAAATTGTAAGGAAGAGATGCAACACGAGAGAGAGGTACTTGANCCATAACCTCTCTAGAATCATGACTTCACNNAATATTCCNGGANTTTNTCTNACTTTTGTTTGTTCNCCANNANATTNNGCAAAATGGNTTGTGGTGGAACCAAATGGGTGGGTTCTTGTTGACTNAGACAAGTTGGTACATAATGACNCNGGNCGTGAGTCCCTACCGGTTCACTCCCACACGGTTCACTNCCACCTTACTAATGTGTTGTCTCTAAAAACCATTCTTCTCNGCATGAAGAACCGAATGAAGACCATCAACACTATTGGGAGNAAGGACATTCNGAAAACTATGATTANAAAGATCAATTGCATCCAAGTAANTGAAGNTGCCATTGCAATCAACTNTAGAGACAGCGCGAACTGCTCCNTNGGCCAAATTATACNCTCGGAAANGAAGCTTTCTAAGTTCGAGGTAACAATCCATTAGATACTTTTTCGATTATTTTAGTAANTTTTTTATTTATTNTNGGNTTTNTTNTAAGTCCGAGGTAGCACTCCGTTACTTTTGCTTTAATANTGATTATCAAAACTTTCATATTCTAGCNAAATNTTAGTAGCAAGATCAGATTTTNTACAATTANTTTTTCAAATCAAAGACACAAACTACTAACATAATCGAGAACAGATGGAAGACTTCTTGGAGTGATGGAATGCCTCTCATCCCACGGGCTCAGTACTTATTCACAAGCTCATAAGACCTGCTAAGTCATTAGATTAAGGAGCTAATAGCTTGCAAGGAAAGTCACGTCCATG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4712600_4712991_wil-2_ler-0_20406_3015_TRUE
SEQUENCE_TARGET=950,491
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4724560_4724576_oy-0_ler-0_22139_15024_TRUE
SEQUENCE_TARGET=950,116
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_6147437_6147442_bur-0_wu-0_98_1719824_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=TGTGTTTTCTAAGCTACNCNNNNNNNNNNATGCTATAAAGTATCAACCTTTAATTAGAAAACTAGTTTAAAGTNCCAAAGGTTATGTTTTCAGAACTGCCCAGAAACATATATATGTACTTGTTAGAACTTGATTTNNTTTTTTNATTGGTGATGTGAGGATTTAGTCNACAGGATCGTTCTTCCATGACAGAAGCATCACACTTGGAACGCTTATGGGATTTAGTTTCACNGCAACTATGCCGATGCCATTNAGAGCNTCTTCACGCCGCCACGTGTCACCTTCCGTCGCAATCTCACGAGCTTCTAGCTCCAATGCAAGACGCAACCACCAGAGAAAGCGTCCTCCTTCAAATTCGGCTGAGCCTGAGCCGCATCGCCGCCGTAAATGGTGGCGGTTTTGCCGAGATGACGATGACGACGCCGCCGGAAATGGAATCCACCGTGGAACAGGAGATTCAAAGCGGTCATCGCTNGGGGAGTATNTNGAGGTGGAACGGAGGTTTGGAGATGAAGCCGTTNACAACTCCGCCGAGGCAGAGCTAGAGGATGCGGTGGTGGCACGGTACCAAGATCAGCAGCCGGTGATGGGGGAGCGGGCATTGTTCGCTGACGGAAGGGTTCTTCCTCCGGCTTCAGNTGAAGNGGTTACCGGAGAANGNACGCCGGTNGCCACGNCCCTTTGTAGATTTCCGGNNTCTTTAACCNGTATATGCAGCGGTGGNGGAGGNTGAATACGTCGGTTCATGTGTGNGNNNNNNATTTGTATTTAGACTTAAATTTTTGTATNNNNNNNNNNNNNNNNGAATCATTGTTTGTGGAAAATATAACATGTTGNATGTAGTCATTGTCAAANTAATTATTTGAGANNNAANNNNNNNTNNNAAGGAGAATTANNAANNANANTANNNANANNNGNAATANTNNNNNNTNNNCNTNNANNANNNNANACNTGNANANCTNTNGNTNNNAANTGGAGNNNNNCNCTNNATNANNNTNTTNATTTNAAATGNTAANNGTTGNTCTATCANATTGAGTTTTTGCTTCAAANNNNNNNNNNNNNNNNTCTTTATACTAGTCAGTCTTTGGACAATAATTTGACCAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANGAGTGTGCCACATTNGTGGTTANTTTTCCACTAGTATTTCTGAAAGTAAAACAATTAACCACAAATCATCCTCATAACATAAGAGGAGAAGATTCCTAACGACGAAGCAAAATTATCGAACAAGTGGACACACACAAGAAACCGAAAATACGGCAAAATGTTTGTTATGTGTATCCATATTTTATANCAATTGAATTAATAAAATAAACTGTANAAACAAGAAACAATTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAANCCTCGAGTGNTGTTAAAAAAAGGAAGAAGGAAAAAGAATAAAAACTCAAAGGGAACGCAACACACTNAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTNNNCAGCAGCAGCAGNAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGCTGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCACCGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCTGAATGATTTTTTCCTTGGGTAGAGTCAAAATTTTGTTTGACAAATGTTGGAATCTGCGTTGACTCGACTCGTAATCTTCTCCGGCGCACTACATGTCTGATTCTGAGCTTTCGAAAGTATGGTTGATTGATTTTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.106709
PRIMER_LEFT_0_PENALTY=0.049859
PRIMER_RIGHT_0_PENALTY=0.056850
PRIMER_LEFT_0_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_0_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_0=493,20
PRIMER_RIGHT_0=1298,20
PRIMER_LEFT_0_TM=60.050
PRIMER_RIGHT_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=806
PRIMER_PAIR_1_PENALTY=0.243451
PRIMER_LEFT_1_PENALTY=0.186600
PRIMER_RIGHT_1_PENALTY=0.056850
PRIMER_LEFT_1_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_1_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_1=446,20
PRIMER_RIGHT_1=1298,20
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=853
PRIMER_PAIR_2_PENALTY=0.311463
PRIMER_LEFT_2_PENALTY=0.254613
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_2_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_2=531,20
PRIMER_RIGHT_2=1298,20
PRIMER_LEFT_2_TM=60.255
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=768
PRIMER_PAIR_3_PENALTY=0.410901
PRIMER_LEFT_3_PENALTY=0.254613
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_LEFT_3_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_3_SEQUENCE=GTGTTGCGTTCCCTTTGAGT
PRIMER_LEFT_3=531,20
PRIMER_RIGHT_3=1500,20
PRIMER_LEFT_3_TM=60.255
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=970
PRIMER_PAIR_4_PENALTY=0.422170
PRIMER_LEFT_4_PENALTY=0.365320
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_4_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_4=451,20
PRIMER_RIGHT_4=1298,20
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=848
PRIMER_PAIR_5_PENALTY=0.463402
PRIMER_LEFT_5_PENALTY=0.049859
PRIMER_RIGHT_5_PENALTY=0.413543
PRIMER_LEFT_5_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_5_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_5=493,20
PRIMER_RIGHT_5=1295,20
PRIMER_LEFT_5_TM=60.050
PRIMER_RIGHT_5_TM=59.586
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=803
PRIMER_PAIR_6_PENALTY=0.463402
PRIMER_LEFT_6_PENALTY=0.049859
PRIMER_RIGHT_6_PENALTY=0.413543
PRIMER_LEFT_6_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_6_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_6=493,20
PRIMER_RIGHT_6=1296,20
PRIMER_LEFT_6_TM=60.050
PRIMER_RIGHT_6_TM=59.586
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=804
PRIMER_PAIR_7_PENALTY=0.463402
PRIMER_LEFT_7_PENALTY=0.049859
PRIMER_RIGHT_7_PENALTY=0.413543
PRIMER_LEFT_7_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_7_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_7=493,20
PRIMER_RIGHT_7=1297,20
PRIMER_LEFT_7_TM=60.050
PRIMER_RIGHT_7_TM=59.586
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=805
PRIMER_PAIR_8_PENALTY=0.478724
PRIMER_LEFT_8_PENALTY=0.049859
PRIMER_RIGHT_8_PENALTY=0.428866
PRIMER_LEFT_8_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_8_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_8=493,20
PRIMER_RIGHT_8=1286,20
PRIMER_LEFT_8_TM=60.050
PRIMER_RIGHT_8_TM=59.571
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=794
PRIMER_PAIR_9_PENALTY=0.530362
PRIMER_LEFT_9_PENALTY=0.254613
PRIMER_RIGHT_9_PENALTY=0.275749
PRIMER_LEFT_9_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_9_SEQUENCE=TGCGTTCCCTTTGAGTTTTT
PRIMER_LEFT_9=531,20
PRIMER_RIGHT_9=1496,20
PRIMER_LEFT_9_TM=60.255
PRIMER_RIGHT_9_TM=59.724
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=966
PRIMER_PAIR_10_PENALTY=0.530362
PRIMER_LEFT_10_PENALTY=0.254613
PRIMER_RIGHT_10_PENALTY=0.275749
PRIMER_LEFT_10_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_10_SEQUENCE=TTGCGTTCCCTTTGAGTTTT
PRIMER_LEFT_10=531,20
PRIMER_RIGHT_10=1497,20
PRIMER_LEFT_10_TM=60.255
PRIMER_RIGHT_10_TM=59.724
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=967
PRIMER_PAIR_11_PENALTY=0.530545
PRIMER_LEFT_11_PENALTY=0.473695
PRIMER_RIGHT_11_PENALTY=0.056850
PRIMER_LEFT_11_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_11_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_11=494,20
PRIMER_RIGHT_11=1298,20
PRIMER_LEFT_11_TM=59.526
PRIMER_RIGHT_11_TM=59.943
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=805
PRIMER_PAIR_12_PENALTY=0.567181
PRIMER_LEFT_12_PENALTY=0.510331
PRIMER_RIGHT_12_PENALTY=0.056850
PRIMER_LEFT_12_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_12_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_12=440,20
PRIMER_RIGHT_12=1298,20
PRIMER_LEFT_12_TM=60.510
PRIMER_RIGHT_12_TM=59.943
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=859
PRIMER_PAIR_13_PENALTY=0.600143
PRIMER_LEFT_13_PENALTY=0.186600
PRIMER_RIGHT_13_PENALTY=0.413543
PRIMER_LEFT_13_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_13_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_13=446,20
PRIMER_RIGHT_13=1295,20
PRIMER_LEFT_13_TM=59.813
PRIMER_RIGHT_13_TM=59.586
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=850
PRIMER_PAIR_14_PENALTY=0.600143
PRIMER_LEFT_14_PENALTY=0.186600
PRIMER_RIGHT_14_PENALTY=0.413543
PRIMER_LEFT_14_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_14_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_14=446,20
PRIMER_RIGHT_14=1296,20
PRIMER_LEFT_14_TM=59.813
PRIMER_RIGHT_14_TM=59.586
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=851
PRIMER_PAIR_15_PENALTY=0.600143
PRIMER_LEFT_15_PENALTY=0.186600
PRIMER_RIGHT_15_PENALTY=0.413543
PRIMER_LEFT_15_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_15_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_15=446,20
PRIMER_RIGHT_15=1297,20
PRIMER_LEFT_15_TM=59.813
PRIMER_RIGHT_15_TM=59.586
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=852
PRIMER_PAIR_16_PENALTY=0.615466
PRIMER_LEFT_16_PENALTY=0.186600
PRIMER_RIGHT_16_PENALTY=0.428866
PRIMER_LEFT_16_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_16_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_16=446,20
PRIMER_RIGHT_16=1286,20
PRIMER_LEFT_16_TM=59.813
PRIMER_RIGHT_16_TM=59.571
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=11.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=841
PRIMER_PAIR_17_PENALTY=0.635218
PRIMER_LEFT_17_PENALTY=0.578368
PRIMER_RIGHT_17_PENALTY=0.056850
PRIMER_LEFT_17_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_17_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_17=341,20
PRIMER_RIGHT_17=1298,20
PRIMER_LEFT_17_TM=60.578
PRIMER_RIGHT_17_TM=59.943
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=11.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=958
PRIMER_PAIR_18_PENALTY=0.668156
PRIMER_LEFT_18_PENALTY=0.254613
PRIMER_RIGHT_18_PENALTY=0.413543
PRIMER_LEFT_18_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_18_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_18=531,20
PRIMER_RIGHT_18=1295,20
PRIMER_LEFT_18_TM=60.255
PRIMER_RIGHT_18_TM=59.586
PRIMER_LEFT_18_GC_PERCENT=60.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=765
PRIMER_PAIR_19_PENALTY=0.668156
PRIMER_LEFT_19_PENALTY=0.254613
PRIMER_RIGHT_19_PENALTY=0.413543
PRIMER_LEFT_19_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_19_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_19=531,20
PRIMER_RIGHT_19=1296,20
PRIMER_LEFT_19_TM=60.255
PRIMER_RIGHT_19_TM=59.586
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=766
PRIMER_PAIR_20_PENALTY=0.668156
PRIMER_LEFT_20_PENALTY=0.254613
PRIMER_RIGHT_20_PENALTY=0.413543
PRIMER_LEFT_20_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_20_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_20=531,20
PRIMER_RIGHT_20=1297,20
PRIMER_LEFT_20_TM=60.255
PRIMER_RIGHT_20_TM=59.586
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=767
PRIMER_PAIR_21_PENALTY=0.683479
PRIMER_LEFT_21_PENALTY=0.254613
PRIMER_RIGHT_21_PENALTY=0.428866
PRIMER_LEFT_21_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_21_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_21=531,20
PRIMER_RIGHT_21=1286,20
PRIMER_LEFT_21_TM=60.255
PRIMER_RIGHT_21_TM=59.571
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=756
PRIMER_PAIR_22_PENALTY=0.768641
PRIMER_LEFT_22_PENALTY=0.711791
PRIMER_RIGHT_22_PENALTY=0.056850
PRIMER_LEFT_22_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_22_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_22=448,20
PRIMER_RIGHT_22=1298,20
PRIMER_LEFT_22_TM=59.288
PRIMER_RIGHT_22_TM=59.943
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=851
PRIMER_PAIR_23_PENALTY=0.778863
PRIMER_LEFT_23_PENALTY=0.365320
PRIMER_RIGHT_23_PENALTY=0.413543
PRIMER_LEFT_23_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_23_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_23=451,20
PRIMER_RIGHT_23=1295,20
PRIMER_LEFT_23_TM=59.635
PRIMER_RIGHT_23_TM=59.586
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=845
PRIMER_PAIR_24_PENALTY=0.778863
PRIMER_LEFT_24_PENALTY=0.365320
PRIMER_RIGHT_24_PENALTY=0.413543
PRIMER_LEFT_24_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_24_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_24=451,20
PRIMER_RIGHT_24=1296,20
PRIMER_LEFT_24_TM=59.635
PRIMER_RIGHT_24_TM=59.586
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=846
PRIMER_PAIR_25_PENALTY=0.778863
PRIMER_LEFT_25_PENALTY=0.365320
PRIMER_RIGHT_25_PENALTY=0.413543
PRIMER_LEFT_25_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_25_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_25=451,20
PRIMER_RIGHT_25=1297,20
PRIMER_LEFT_25_TM=59.635
PRIMER_RIGHT_25_TM=59.586
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=847
PRIMER_PAIR_26_PENALTY=0.794186
PRIMER_LEFT_26_PENALTY=0.365320
PRIMER_RIGHT_26_PENALTY=0.428866
PRIMER_LEFT_26_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_26_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_26=451,20
PRIMER_RIGHT_26=1286,20
PRIMER_LEFT_26_TM=59.635
PRIMER_RIGHT_26_TM=59.571
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=836
PRIMER_PAIR_27_PENALTY=0.887238
PRIMER_LEFT_27_PENALTY=0.473695
PRIMER_RIGHT_27_PENALTY=0.413543
PRIMER_LEFT_27_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_27_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_27=494,20
PRIMER_RIGHT_27=1295,20
PRIMER_LEFT_27_TM=59.526
PRIMER_RIGHT_27_TM=59.586
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=802
PRIMER_PAIR_28_PENALTY=0.887238
PRIMER_LEFT_28_PENALTY=0.473695
PRIMER_RIGHT_28_PENALTY=0.413543
PRIMER_LEFT_28_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_28_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_28=494,20
PRIMER_RIGHT_28=1296,20
PRIMER_LEFT_28_TM=59.526
PRIMER_RIGHT_28_TM=59.586
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=803
PRIMER_PAIR_29_PENALTY=0.887238
PRIMER_LEFT_29_PENALTY=0.473695
PRIMER_RIGHT_29_PENALTY=0.413543
PRIMER_LEFT_29_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_29_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_29=494,20
PRIMER_RIGHT_29=1297,20
PRIMER_LEFT_29_TM=59.526
PRIMER_RIGHT_29_TM=59.586
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=804
PRIMER_PAIR_30_PENALTY=0.889843
PRIMER_LEFT_30_PENALTY=0.832993
PRIMER_RIGHT_30_PENALTY=0.056850
PRIMER_LEFT_30_SEQUENCE=CAACCACCAGAGAAAGCGTC
PRIMER_RIGHT_30_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_30=324,20
PRIMER_RIGHT_30=1298,20
PRIMER_LEFT_30_TM=60.833
PRIMER_RIGHT_30_TM=59.943
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=975
PRIMER_PAIR_31_PENALTY=0.889843
PRIMER_LEFT_31_PENALTY=0.832993
PRIMER_RIGHT_31_PENALTY=0.056850
PRIMER_LEFT_31_SEQUENCE=GACGCAACCACCAGAGAAAG
PRIMER_RIGHT_31_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_31=320,20
PRIMER_RIGHT_31=1298,20
PRIMER_LEFT_31_TM=60.833
PRIMER_RIGHT_31_TM=59.943
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=0.889843
PRIMER_LEFT_32_PENALTY=0.832993
PRIMER_RIGHT_32_PENALTY=0.056850
PRIMER_LEFT_32_SEQUENCE=AAGACGCAACCACCAGAGAA
PRIMER_RIGHT_32_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_32=318,20
PRIMER_RIGHT_32=1298,20
PRIMER_LEFT_32_TM=60.833
PRIMER_RIGHT_32_TM=59.943
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=981
PRIMER_PAIR_33_PENALTY=0.902560
PRIMER_LEFT_33_PENALTY=0.473695
PRIMER_RIGHT_33_PENALTY=0.428866
PRIMER_LEFT_33_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_33_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_33=494,20
PRIMER_RIGHT_33=1286,20
PRIMER_LEFT_33_TM=59.526
PRIMER_RIGHT_33_TM=59.571
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=793
PRIMER_PAIR_34_PENALTY=0.919829
PRIMER_LEFT_34_PENALTY=0.862979
PRIMER_RIGHT_34_PENALTY=0.056850
PRIMER_LEFT_34_SEQUENCE=ACGGAGGTTTGGAGATGAAG
PRIMER_RIGHT_34_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_34=495,20
PRIMER_RIGHT_34=1298,20
PRIMER_LEFT_34_TM=59.137
PRIMER_RIGHT_34_TM=59.943
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=804
PRIMER_PAIR_35_PENALTY=0.922928
PRIMER_LEFT_35_PENALTY=0.254613
PRIMER_RIGHT_35_PENALTY=0.668315
PRIMER_LEFT_35_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_35_SEQUENCE=TGTTGCGTTCCCTTTGAGTT
PRIMER_LEFT_35=531,20
PRIMER_RIGHT_35=1499,20
PRIMER_LEFT_35_TM=60.255
PRIMER_RIGHT_35_TM=60.668
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=969
PRIMER_PAIR_36_PENALTY=0.923874
PRIMER_LEFT_36_PENALTY=0.510331
PRIMER_RIGHT_36_PENALTY=0.413543
PRIMER_LEFT_36_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_36_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_36=440,20
PRIMER_RIGHT_36=1295,20
PRIMER_LEFT_36_TM=60.510
PRIMER_RIGHT_36_TM=59.586
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=856
PRIMER_PAIR_37_PENALTY=0.923874
PRIMER_LEFT_37_PENALTY=0.510331
PRIMER_RIGHT_37_PENALTY=0.413543
PRIMER_LEFT_37_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_37_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_37=440,20
PRIMER_RIGHT_37=1296,20
PRIMER_LEFT_37_TM=60.510
PRIMER_RIGHT_37_TM=59.586
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=857
PRIMER_PAIR_38_PENALTY=0.923874
PRIMER_LEFT_38_PENALTY=0.510331
PRIMER_RIGHT_38_PENALTY=0.413543
PRIMER_LEFT_38_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_38_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_38=440,20
PRIMER_RIGHT_38=1297,20
PRIMER_LEFT_38_TM=60.510
PRIMER_RIGHT_38_TM=59.586
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=858
PRIMER_PAIR_39_PENALTY=0.939197
PRIMER_LEFT_39_PENALTY=0.510331
PRIMER_RIGHT_39_PENALTY=0.428866
PRIMER_LEFT_39_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_39_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_39=440,20
PRIMER_RIGHT_39=1286,20
PRIMER_LEFT_39_TM=60.510
PRIMER_RIGHT_39_TM=59.571
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=847
PRIMER_PAIR_40_PENALTY=0.991911
PRIMER_LEFT_40_PENALTY=0.578368
PRIMER_RIGHT_40_PENALTY=0.413543
PRIMER_LEFT_40_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_40_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_40=341,20
PRIMER_RIGHT_40=1295,20
PRIMER_LEFT_40_TM=60.578
PRIMER_RIGHT_40_TM=59.586
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=955
PRIMER_PAIR_41_PENALTY=0.991911
PRIMER_LEFT_41_PENALTY=0.578368
PRIMER_RIGHT_41_PENALTY=0.413543
PRIMER_LEFT_41_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_41_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_41=341,20
PRIMER_RIGHT_41=1296,20
PRIMER_LEFT_41_TM=60.578
PRIMER_RIGHT_41_TM=59.586
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=11.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=956
PRIMER_PAIR_42_PENALTY=0.991911
PRIMER_LEFT_42_PENALTY=0.578368
PRIMER_RIGHT_42_PENALTY=0.413543
PRIMER_LEFT_42_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_42_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_42=341,20
PRIMER_RIGHT_42=1297,20
PRIMER_LEFT_42_TM=60.578
PRIMER_RIGHT_42_TM=59.586
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=11.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=957
PRIMER_PAIR_43_PENALTY=1.007234
PRIMER_LEFT_43_PENALTY=0.578368
PRIMER_RIGHT_43_PENALTY=0.428866
PRIMER_LEFT_43_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_43_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_43=341,20
PRIMER_RIGHT_43=1286,20
PRIMER_LEFT_43_TM=60.578
PRIMER_RIGHT_43_TM=59.571
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=946
PRIMER_PAIR_44_PENALTY=1.036122
PRIMER_LEFT_44_PENALTY=0.254613
PRIMER_RIGHT_44_PENALTY=0.781508
PRIMER_LEFT_44_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_44_SEQUENCE=GTTGCGTTCCCTTTGAGTTT
PRIMER_LEFT_44=531,20
PRIMER_RIGHT_44=1498,20
PRIMER_LEFT_44_TM=60.255
PRIMER_RIGHT_44_TM=59.218
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=968
PRIMER_PAIR_45_PENALTY=1.125334
PRIMER_LEFT_45_PENALTY=0.711791
PRIMER_RIGHT_45_PENALTY=0.413543
PRIMER_LEFT_45_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_45_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_45=448,20
PRIMER_RIGHT_45=1295,20
PRIMER_LEFT_45_TM=59.288
PRIMER_RIGHT_45_TM=59.586
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=848
PRIMER_PAIR_46_PENALTY=1.125334
PRIMER_LEFT_46_PENALTY=0.711791
PRIMER_RIGHT_46_PENALTY=0.413543
PRIMER_LEFT_46_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_46_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_46=448,20
PRIMER_RIGHT_46=1296,20
PRIMER_LEFT_46_TM=59.288
PRIMER_RIGHT_46_TM=59.586
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=849
PRIMER_PAIR_47_PENALTY=1.125334
PRIMER_LEFT_47_PENALTY=0.711791
PRIMER_RIGHT_47_PENALTY=0.413543
PRIMER_LEFT_47_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_47_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_47=448,20
PRIMER_RIGHT_47=1297,20
PRIMER_LEFT_47_TM=59.288
PRIMER_RIGHT_47_TM=59.586
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=850
PRIMER_PAIR_48_PENALTY=1.140657
PRIMER_LEFT_48_PENALTY=0.711791
PRIMER_RIGHT_48_PENALTY=0.428866
PRIMER_LEFT_48_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_48_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_48=448,20
PRIMER_RIGHT_48=1286,20
PRIMER_LEFT_48_TM=59.288
PRIMER_RIGHT_48_TM=59.571
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=839
PRIMER_PAIR_49_PENALTY=1.152585
PRIMER_LEFT_49_PENALTY=1.095734
PRIMER_RIGHT_49_PENALTY=0.056850
PRIMER_LEFT_49_SEQUENCE=CAGAGCTAGAGGATGCGGTG
PRIMER_RIGHT_49_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_49=536,20
PRIMER_RIGHT_49=1298,20
PRIMER_LEFT_49_TM=61.096
PRIMER_RIGHT_49_TM=59.943
PRIMER_LEFT_49_GC_PERCENT=60.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=763
=
SEQUENCE_ID=MAGIC.433_4_4485512_4485778_oy-0_can-0_36132_17681_TRUE
SEQUENCE_TARGET=950,366
SEQUENCE_TEMPLATE=AAATGNTTAAAATAGTAAAATNATACATCNAAAATATTTGAACNGGGAANATGGGGAAATNTAATTCGGATGAGAGGNAAAATTGCCGCCAAAACACAACAAATATCAACTNNTTTTTTNGGAAACAAATATCCANGCNGATTGTNNTNTTTTNATTACTANACTGATNTAAAAAANTTAAATNAATTTTATANAAAAAAATCAAAACCAACGANAGANTTGCCAATATTATACATAGACCAAACNGGTTNTATACCANNCATCGATGTNTAAGTTATTGGTATAATAAAGTCTATAAATNGAACAAAACCTTNAAACATTCTTATAAAAAGATGAATCGTTTAACTGAAGATAAGGTGGGCGATTTACGGGAAAGCAAGGTTTATAANAATGTTAATTNNTATAATGATTGTGANNAAATTTCTTNATTTAATATGTAGGAGATTCCAANGATGGATATACACATTCCTCAGCCGTTGATGGAGATCGTCGTTAGTNAAGTTGCTGAAGATGGTGTNGATGCTTTAAAGAACTGGTTNNNAGNTNGGAGANANGNTCNAACCAAGANGAAGCTTNAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_4452729_4452769_wil-2_po-0_10164_50546_TRUE
SEQUENCE_TARGET=950,140
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4503041_4503113_can-0_ct-1_21879_1248_TRUE
SEQUENCE_TARGET=950,172
SEQUENCE_TEMPLATE=TACCCTANAATTCCCNTTGAGCTTACTTGTTNTACCACTACATTCTCGACAATCACCGATGTTTCTAGATCGGGGACCAAGCTTAAAAAACGTTTTGTGATATGCATTCTTTTTCTGAAATGGCCACAGCCTATGAGGAGTATTCCATACGCAAAATGGGCAAACAATGAATGTACCACACATAAACGTAGCATATGAAGAGCTTCTTTTGAGGATGGACTATCTTGAGTCAATATNCTCAACGATTCTACTTACCNTGCTGTGGATGTCAGATTCACACANTCAAGTATTTNTTTATTTAGTGAGAAGGATTATTCTTNAGGACAAATGTANTNGAAAATAGATTGATGATTATAGAGAAAACTATGAGNTTTNTAANAAAATGTGTATANNANNTTTGCACAAAAATGCNNGTNNNNCTCTACATGGTCTTTGACCCACAATTTATACTCAATGAATACATTTAATTACCATTTATACACAATTATTCTATATTAAATGTGTCTGTTTCAGTCTTCAAATTAACTATTCGAACGTTCGAATTTGAATNGNTTCCAAAATTTCTGGGCTTCGTCCTCTGGCCCANNNGACTCTCCAATCCGGGCAGTTCTGACATCGATGTCCAGTCCACCTNGCNNNNCNNTCACCTCACNTNTACTTTTACTCAGAAAAGCCCATGGGCCTTTCCTCTTTTAGATCNNTNCNNCTTACGNGGCGATCTTTGACCGNTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNATNCCTNAATGNNCAAAGATNNNNCNNAATGACTCTCTGANTTCCCGCCAANNNNNNNNNNNNNNNNNNNNNNNGGAATCACCCATAATTACTCTCGTGACATCTGTCANAGTTATGACCNCATANCAATTATNTACTATTNAANCGGNAAAAACTNTTCCACNTCCTCCANGACTNCCAAGGCNGTTNCGTCATNATCNCTAGCTTGCGTAAGGTAANTTCNCTTTNNNNNNNNNNNNNNNNNNNNCANNNTTNTTTTANAACAAAGCNCCTNTCTTCTTCCTNNNNNNNNNNNNNNNNNNNNGAACCCTCAGACNANNCATTTTTANGATNAATTCNNAAANTGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAANGANNTTTGCACNCGNNNGAATGANATNGCCGNNCCNCANTANCANTCCACNTGNACNCTNCAANCCCTNAATNGATTGNGAGAGCTNTGTCGNNNNNNNNNNNNNANTATGAGGGANTCNCANATGCCNNANCCNANAGANTCGCCGNAAGACCANNNTNCNGGGTTCTTCTNCNTGTNCGANATNTANTTCAAGGGTTGCGGTNTGANNTTTNCNCTTCCNGNAGCCCTNGTTCNGTACTTANCNGCCCTNNAAATTGCCNTGCCCNNNNTNACCCCTNACNTNCTNCGNNCTATNTTAGNGATTATTACNATCGCNNCNNANGCCGGATNTGTNATCNGNGTTCCNNAACTGAATGAACTTCTCAGCNTGAGGAGTTCTTCNAAGAAAGNNGNATACTTCTNGGCTTATNCGAANGCCNACANGAANCTCATCTNNNATCTTNCNAACAANGACGANAACTNGCANCANCCNTGGTTNNTAANNNNNNNNNNNNNNNNNNNNNTNGNAAATCTTTCCGACNTGCTCCCCNNGAAGNCNNGTAGNNCTTAGGTCATGCNTATGATTTTTATANANATNTTGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGAAACTCTTTGGAATTCCTTCACCNTNGACNGNTTCATANAGGCNAACCACAAGCTCANGANGAGTCNCCCTGNACAACTTCTTCAACTTCNGTTNCCTCCTCCTTTACCTCANNGANCNTCNNCTCGAGNCATCGCCNCCNGANGNAAGANGNTGTCCAAATCNATCGTGGAGGCTTGTGANGNGAAC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_12694795_12694805_mt-0_ct-1_25306_13246_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NCGATTCACCCNAGAACTCGATNTNCATCTNATTCTTCTTCTNNNNNNNNNNNAAAANAATGTCAGCTAGTCAGAGCTNNAAATCTGATGCCAACAACAACAAGATGAGTTTCGNGCAGGGAAGTTATGGACATTNGAGCTNNNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_15173025_15173173_zu-0_mt-0_51207_330990_TRUE
SEQUENCE_TARGET=950,248
SEQUENCE_TEMPLATE=TANNNNTTNTAATGAGNAGATCAACATATTCAGTTTGCTTTNATCATATCCAATGANCATCNTTTTCTNATTATTATTTNATGGAGATGCCANGGAAACACTCTCAGCCNTGNGNNCCTNACTCTACAACNNNGAAGNTGNACGACANAGACTAACCCTTNTGCTTTCNTCTACATCNAAGATNAAGACNCGGTTAGGAGGCAGGGGTATCNNTGAGTTAGTAGGGACACATAAAGGGTCAGACTTTGTGAGGTAAATGAAGAGATCGCNCATGTCTTGAGTGTAAACAGCATTTGCTTCTTCGTCTAGCTTGAACACCANTAAAGCNNTTGTTTTCNATTTCCCAATACCCTTGATGATCNTTCTAGAGGANTTTTTATACCACTTAACCAANAACATTTCANTGGTGGTTCTTAACCAAGTGTTCGCTCCTGCAGCTCGCGTTCGGTCTTAGTCAGTTTGGAAANNTTTTNGTNCCGCAGCNTCTGAAACTTGGGNNNNNNNNNNNNANTGTNGANATCCCATGATCCAATGAGGTGGCCTCCGGATCCAGGTATGCNAAACANGCCATTCTNNGAAANCANAACCGAAAAAGAGAAGAAGCANGGGTTTTTAATTTTGATATTGGTCCACTTGGAGTGGCNTTCATCNGGTCTGNANAGCTNAGTTGAGGTACCAAGAACTTGATAGCCANAACANAGTCGTCTTCCTCGGGAGAAGNTGNGGACATGGCCACGTTGGTGAGAATTTGGGTTTGANAATGAGGCAGCTTTACAAGTGGAGGCAGCGGAATGTGTTTCGGTTCAGATGTCAACCCTACCAAGTTTGGAGANGCAAAACTTGATGANAAAGCCAAANTTTTTTAGTGAATTAGCACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTNTATGTATATATTGNANCANATANATCNNTNTTTTTTTGTNTACTTTTTATGAANNTTCACACNTTATAATTAGTCNTATTAGTTATTATTNTTTATTTTNTGTTGTATATAANGGANAAAGTTGNNTATATACAATCTCAAANAAATANNTTATTGCATCATTTCNAGTATATTATAATTTTATGAAGAAGTANTGAAATAACATGTCTAACATAATCNNAAAATTTNAAATTCATTATATATACAAGAAACNATNTGTANAAAACTAANANTTTCGANTCNTTTCCGATTNTANNCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_9605694_9606202_sf-2_bur-0_4808_7989_TRUE
SEQUENCE_TARGET=950,608
SEQUENCE_TEMPLATE=AATACGTCATGNNACTTGTCTTCTTCCCATAAGAAAGAGGGAGTATGTGTTTATCATGCTTATACCTCACTTGTTGTGGTAAAGTAGCACATTTGAAACACAAAGCANAGTCACACTCCTNCTCNATGCAATTGAAGGTTTCCTTGGTGTACTCNCNNGGCGCTTTGGCGCAAACCGAACAGTTTCTTTCCTCATTTGGTTTGGATGTTAGGAATAAATGATGCATATGACTTGGATGAAGTAAAGGCTCNGAAATTGTGGCGCACTGCACATGTAGCCTAAAATCGCAATNTNTTTTACTGCANTNNTANAAGAAACCAGNTATACACAACGAAGGACAAGCTGAACANTAATCACGATCAGAATTANTCCAAGNNCCAACANNTCTGAGAGGGTTATNTGATANTAAAGTGAGTAGATGTGGATGTATTGGATGATGTAANCTNCGAGANAGATTTGCACATTCTTCNTGTANAATGAANTCACATTGCAAACAAGAGTANGAGTTACCAAAATAAATNGGCATGATGCATGCTTGACACAGCTTATTCTCGTCGTAATCTTTGCCGNTGTTCTCATCANGTGTCAAATGATGATGTTCATGACTGAAATGNTGTATGATTCCATCGCTTATCGTCANGAATGGCTCAAGTTCTTCCACTTCTTCTTCTGACTCTCCTTCACGTTCTATACCATCCCAAACATTTGNTTGAGTGGCACATTTTGAATGNACCGCATAAAGACAACCTTTCTTGATGNAATGATANCCCCCGTAATCATTGTTAATCTTCCGGCGNCAAANACCACAAATCCAATCTCCTTTTNGGAAAGAATGGGTAAAANAGATANAATGGAGGTGGCGAGAAATCCGTATGACATGAGGTAAGCTGAAACAGCTTTGATGGANNATAAAGTNGCAAGGGGGACATATATAGAANANCAAATTTGAATGTTTCAAGGCNCANAGGCTGCAAGTTTGGGGATCNNGACTTGGAAACAGNGCAAGTGTATGCTNATGCCACTTGGGTTGGTCNATAGACAAGACTGGTGATTTCTTNGCACATGTAAANTTGAGCCCAAAATCGNAAGNAGAGCAAAAATAAAACATACTTGCNGTATCGTCANGGAGANNTTCGTGACAGCAATANCATTGCCTGGTTTTGTCATCATTGAACAAGGCAAGCNNGAGAGAATGTTTNGGATGAAGAGGGTGTTTGATCTCNNCNGNNNNCTTNCNNNTATNTTCATGGTTCTCNCCNTAGCAACCTCGGCAGANNAAATNGGTNNNNNNNNNNNNNNNNNNNNNNATGANCTTNTCCCAGTTNAANGGNAGACAGTCCCANTTATTNNNTCTCCAGNANACTNCTCGTGCTNCAGTNNNNNNNNNNNNNNNNNNTNGATNGTCCNNCTTTTGATTCCTGNTCCATAAGCNTTNTTCAATCACATGAAATTTTCCCTNNTTCANNATGACTTGTACTTGAGGGCAAAGGGAAANAANANTCTTNNACNTTGAATTCATAGNCANGACGNGGGAGAATATACGATGGATGAGTGATATGAGCTTNGGCTCNGNNTCNAGACTCAATNTTTCCGNAANCANNANAANAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNNNNNNNNNNNNNNNNNNNNNNNNNGGCTGAGAATCAANATCCATAGAGTTGAAGACAGAGANAGATTGATTNNTGAGTGANATGAGCTCCGACTNNGGCTTCGGCTGCAAATTCCANTCNACTGAGNTGNCCNGAGAGATTATATATCTACTGAGTGACAAGANNNNNNNNNNNNNNNNNNNNNNCNGTCTNTNAGACANNTTATCGTCCANAAAGATTAGTTGANTNATGANTGATTTGAGNTTCAGCTCCGGAGANTTGTCGACGAGGGATATTATTTTNTNAATGAGTGATATGAGCTCCGGCTGCGGCTTNATNCTCCATGTNTCCNTGATTAGTGNCATGAGNNTANANTNCGGNNNNNGCTNNTCCCAANNTGNAGAGTTGACNAANNGCGTTCNAGAAANGAGTGATATGAGCNTCCTNAGCGNNTTNGGCTGGGACTCCAAATCCATNTCCNTGAAANAANCGATTGTTTCAGAGATGAGTGACATCAGCTCCNTCTCCGTCTNNNNNNNCNTTGACTTAACCAGAGNGATTNTTTGANTAATCANTNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNAANNCATTGANNNNNANGATNAGTAGTATGANCTTCNCCTCCGGATCCATCGNGANTTNCCGAGANNAAATTGTGAGANTANGATGAGAGAATTTTCANTATGAATGNAANTTANTGNTTGCTCTCTGTGTTTGTGAATGCTAATTGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_900903_900949_ler-0_zu-0_5716_79066_TRUE
SEQUENCE_TARGET=950,146
SEQUENCE_TEMPLATE=TTTAATAGTATGAATTCATTGACATCAATTNAGCATGATGAGGNGGTCTACACTTNAAAATATAAANTAATTAATNTGTATGAGACTATGGGTTTTAGACTNCTAGTGANTACGTTCATATTTAATTTTGGGTGCGTTCCAAANATATGTAATCATATAAGATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATACTATGAATATGACTTGTAATAAGGCCGACTAGTNTAACAAAGTAACGACTAACGAGGCCCATTTTAGGCTGTGTTACGTAGTTCAANTTTTAGGCCTACAATACGTATGTGANNNNNNNNNGCTACAACAACACAAACATTTATGTAATTCNACGATAACANANNNNNNNNNNNNNNNNNNTAACACCAAACTATGGCAGAGNCTGAATTTCTTTCAGAGTTACCGAGTCACNGGACTGAATCGATTGGATTATTTNTAGGTCATTTGATAAANTTAGTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATATATCAAAATTATTTATCATAAAATCAAATTTCAAATATATNATTTCAAAGACAATTAAAATAGCAACTCAAACACAACACAGGCCACCCAAATCTGAACCAACATAACATATNGACTTATTGACAATAATCGGGGTGGAAAAAACACACACGAACAAACAATCAAGAATCAGATTATATGAAAANNCAAAAAAAACAAATTTAACTATCGTTGAATTCCCAACAATTTGTTCTGATCTCACCGTCTTCACCTGTGAGGACACTAACGGCTCCCACTTTGAATAAGTCATTGNCGTCTGCNTATTGAGCCACCACACTAGAAGTGGCTTCGTCGGTTGTGAGCCGTTGGTCAAGTTGCAAAACTCCTCTCCTCGCTCGGATTTTCCCAAAGAATGCGTGTTATCAAAGTCAACGGTGTTGACCTGTCCAGTCNGGTTGACGGGTTCCTGAGGCCGCACATATGCTGGTTAAACTGTATNTATAAAGCTTTCAAATATTAATTGTTAAGTTCGTTTATTTCATCCATTGCTTATATACTCTGGTTGATAGGATAATTAATTAGCAAAGNTTGGACTATGAGTTATGAGAACTATGAGAATCTCATAATGAGATCCACTANAGATAACTATACATACGTGTAAGTCAAACTTATTTAAATTAGAGAGGTGAAACTATAAACGTAAAGGGAAACATTCCGGTGCCTTTGGATAAACAGTATATATATGATGTGTATTGATGATGTACTTAAAGAGATAATAATACTTATAAGCAAAAGTAGATATCGACAGAATCTTTAATCTTTTATTGTCNGTAAGATGTATGTTTCAACTAGTTTAGATTTTCCTATTTTCTTTGTAAAAATAATTTATTTTNTCGTAAAATTTATGTGGTAAACNATAAATGTCGGAAAATGTCAGCTGCTTGCTGCTAAATATTGAAAGTATCCATANTGCGTTTTAGTTGGTTTCGTCGTTTCGATATTGAAGAAAACAAAAATCAAGAAATAAAATATGTATTATATATTAGTAAAGCTAAGTTAGACTCTACAACTAATTCTATAACTTTGAATTGTTCTATTGGATTCTTCAACAGTGGTAAGACTTCCGCATGGTTCCGAAAGAGATGAATTCAATTATTGGACAGTTTTAACTCTTAAGATCTTATAAAAAATCTTTATAATCTTTACAAAATCTTGAGAGAATGGAACTTTCTATAATTCAAATTAATTGGATAGAGCCAGAAAATAAATTACGTANNNNNNNNNNNNTGAGCATGAAAAATATGCAACTCTGAAGTTAGTTTTGGACTCTAAAAAACTAAAATACATCACAATTCACTCAAAACTCGTACCAACATAACATATTNTTGTTACCAAATCGATTCACCAAACCTTATTTTAAAGATATACGTACANACATATATAGGCAAACAATCACCATTCACAATTCATCATCAGAAGCGGAAATTAAGGGAAAAAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.232211
PRIMER_LEFT_0_PENALTY=0.209870
PRIMER_RIGHT_0_PENALTY=0.022341
PRIMER_LEFT_0_SEQUENCE=CCACCCAAATCTGAACCAAC
PRIMER_RIGHT_0_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_0=652,20
PRIMER_RIGHT_0=1546,20
PRIMER_LEFT_0_TM=60.210
PRIMER_RIGHT_0_TM=59.978
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=895
PRIMER_PAIR_1_PENALTY=0.288056
PRIMER_LEFT_1_PENALTY=0.265715
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=ACTAACGGCTCCCACTTTGA
PRIMER_RIGHT_1_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_1=829,20
PRIMER_RIGHT_1=1546,20
PRIMER_LEFT_1_TM=59.734
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=718
PRIMER_PAIR_2_PENALTY=0.389646
PRIMER_LEFT_2_PENALTY=0.367306
PRIMER_RIGHT_2_PENALTY=0.022341
PRIMER_LEFT_2_SEQUENCE=CAATAATCGGGGTGGAAAAA
PRIMER_RIGHT_2_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_2=692,20
PRIMER_RIGHT_2=1546,20
PRIMER_LEFT_2_TM=59.633
PRIMER_RIGHT_2_TM=59.978
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=855
PRIMER_PAIR_3_PENALTY=0.400189
PRIMER_LEFT_3_PENALTY=0.377848
PRIMER_RIGHT_3_PENALTY=0.022341
PRIMER_LEFT_3_SEQUENCE=GACAATAATCGGGGTGGAAA
PRIMER_RIGHT_3_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_3=690,20
PRIMER_RIGHT_3=1546,20
PRIMER_LEFT_3_TM=59.622
PRIMER_RIGHT_3_TM=59.978
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=857
PRIMER_PAIR_4_PENALTY=0.453398
PRIMER_LEFT_4_PENALTY=0.431057
PRIMER_RIGHT_4_PENALTY=0.022341
PRIMER_LEFT_4_SEQUENCE=ACGGCTCCCACTTTGAATAA
PRIMER_RIGHT_4_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_4=833,20
PRIMER_RIGHT_4=1546,20
PRIMER_LEFT_4_TM=59.569
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=714
PRIMER_PAIR_5_PENALTY=0.530372
PRIMER_LEFT_5_PENALTY=0.124655
PRIMER_RIGHT_5_PENALTY=0.405717
PRIMER_LEFT_5_SEQUENCE=TAGAAGTGGCTTCGTCGGTT
PRIMER_RIGHT_5_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_5=885,20
PRIMER_RIGHT_5=1489,20
PRIMER_LEFT_5_TM=59.875
PRIMER_RIGHT_5_TM=60.406
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.9000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=605
PRIMER_PAIR_6_PENALTY=0.615586
PRIMER_LEFT_6_PENALTY=0.209870
PRIMER_RIGHT_6_PENALTY=0.405717
PRIMER_LEFT_6_SEQUENCE=CCACCCAAATCTGAACCAAC
PRIMER_RIGHT_6_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_6=652,20
PRIMER_RIGHT_6=1489,20
PRIMER_LEFT_6_TM=60.210
PRIMER_RIGHT_6_TM=60.406
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=838
PRIMER_PAIR_7_PENALTY=0.632923
PRIMER_LEFT_7_PENALTY=0.610582
PRIMER_RIGHT_7_PENALTY=0.022341
PRIMER_LEFT_7_SEQUENCE=CACCCAAATCTGAACCAACA
PRIMER_RIGHT_7_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_7=653,20
PRIMER_RIGHT_7=1546,20
PRIMER_LEFT_7_TM=59.389
PRIMER_RIGHT_7_TM=59.978
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=894
PRIMER_PAIR_8_PENALTY=0.638490
PRIMER_LEFT_8_PENALTY=0.616149
PRIMER_RIGHT_8_PENALTY=0.022341
PRIMER_LEFT_8_SEQUENCE=TCAAACACAACACAGGCCAC
PRIMER_RIGHT_8_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_8=636,20
PRIMER_RIGHT_8=1546,20
PRIMER_LEFT_8_TM=60.616
PRIMER_RIGHT_8_TM=59.978
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=2.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=911
PRIMER_PAIR_9_PENALTY=0.671432
PRIMER_LEFT_9_PENALTY=0.265715
PRIMER_RIGHT_9_PENALTY=0.405717
PRIMER_LEFT_9_SEQUENCE=ACTAACGGCTCCCACTTTGA
PRIMER_RIGHT_9_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_9=829,20
PRIMER_RIGHT_9=1489,20
PRIMER_LEFT_9_TM=59.734
PRIMER_RIGHT_9_TM=60.406
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=661
PRIMER_PAIR_10_PENALTY=0.701732
PRIMER_LEFT_10_PENALTY=0.679391
PRIMER_RIGHT_10_PENALTY=0.022341
PRIMER_LEFT_10_SEQUENCE=CACTAACGGCTCCCACTTTG
PRIMER_RIGHT_10_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_10=828,20
PRIMER_RIGHT_10=1546,20
PRIMER_LEFT_10_TM=60.679
PRIMER_RIGHT_10_TM=59.978
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=719
PRIMER_PAIR_11_PENALTY=0.810574
PRIMER_LEFT_11_PENALTY=0.788233
PRIMER_RIGHT_11_PENALTY=0.022341
PRIMER_LEFT_11_SEQUENCE=GACACTAACGGCTCCCACTT
PRIMER_RIGHT_11_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_11=826,20
PRIMER_RIGHT_11=1546,20
PRIMER_LEFT_11_TM=59.212
PRIMER_RIGHT_11_TM=59.978
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=721
PRIMER_PAIR_12_PENALTY=0.836774
PRIMER_LEFT_12_PENALTY=0.431057
PRIMER_RIGHT_12_PENALTY=0.405717
PRIMER_LEFT_12_SEQUENCE=ACGGCTCCCACTTTGAATAA
PRIMER_RIGHT_12_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_12=833,20
PRIMER_RIGHT_12=1489,20
PRIMER_LEFT_12_TM=59.569
PRIMER_RIGHT_12_TM=60.406
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.2000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=657
PRIMER_PAIR_13_PENALTY=0.860277
PRIMER_LEFT_13_PENALTY=0.837936
PRIMER_RIGHT_13_PENALTY=0.022341
PRIMER_LEFT_13_SEQUENCE=CAAAACTCCTCTCCTCGCTC
PRIMER_RIGHT_13_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_13=925,20
PRIMER_RIGHT_13=1546,20
PRIMER_LEFT_13_TM=59.162
PRIMER_RIGHT_13_TM=59.978
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=622
PRIMER_PAIR_14_PENALTY=0.878184
PRIMER_LEFT_14_PENALTY=0.209870
PRIMER_RIGHT_14_PENALTY=0.668315
PRIMER_LEFT_14_SEQUENCE=CCACCCAAATCTGAACCAAC
PRIMER_RIGHT_14_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_14=652,20
PRIMER_RIGHT_14=1545,20
PRIMER_LEFT_14_TM=60.210
PRIMER_RIGHT_14_TM=60.668
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=894
PRIMER_PAIR_15_PENALTY=0.887498
PRIMER_LEFT_15_PENALTY=0.865157
PRIMER_RIGHT_15_PENALTY=0.022341
PRIMER_LEFT_15_SEQUENCE=ACAATAATCGGGGTGGAAAA
PRIMER_RIGHT_15_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_15=691,20
PRIMER_RIGHT_15=1546,20
PRIMER_LEFT_15_TM=59.135
PRIMER_RIGHT_15_TM=59.978
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=856
PRIMER_PAIR_16_PENALTY=0.934030
PRIMER_LEFT_16_PENALTY=0.265715
PRIMER_RIGHT_16_PENALTY=0.668315
PRIMER_LEFT_16_SEQUENCE=ACTAACGGCTCCCACTTTGA
PRIMER_RIGHT_16_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_16=829,20
PRIMER_RIGHT_16=1545,20
PRIMER_LEFT_16_TM=59.734
PRIMER_RIGHT_16_TM=60.668
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=717
PRIMER_PAIR_17_PENALTY=1.016299
PRIMER_LEFT_17_PENALTY=0.610582
PRIMER_RIGHT_17_PENALTY=0.405717
PRIMER_LEFT_17_SEQUENCE=CACCCAAATCTGAACCAACA
PRIMER_RIGHT_17_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_17=653,20
PRIMER_RIGHT_17=1489,20
PRIMER_LEFT_17_TM=59.389
PRIMER_RIGHT_17_TM=60.406
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=837
PRIMER_PAIR_18_PENALTY=1.021866
PRIMER_LEFT_18_PENALTY=0.616149
PRIMER_RIGHT_18_PENALTY=0.405717
PRIMER_LEFT_18_SEQUENCE=TCAAACACAACACAGGCCAC
PRIMER_RIGHT_18_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_18=636,20
PRIMER_RIGHT_18=1489,20
PRIMER_LEFT_18_TM=60.616
PRIMER_RIGHT_18_TM=60.406
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=9.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=854
PRIMER_PAIR_19_PENALTY=1.035620
PRIMER_LEFT_19_PENALTY=0.367306
PRIMER_RIGHT_19_PENALTY=0.668315
PRIMER_LEFT_19_SEQUENCE=CAATAATCGGGGTGGAAAAA
PRIMER_RIGHT_19_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_19=692,20
PRIMER_RIGHT_19=1545,20
PRIMER_LEFT_19_TM=59.633
PRIMER_RIGHT_19_TM=60.668
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=854
PRIMER_PAIR_20_PENALTY=1.046162
PRIMER_LEFT_20_PENALTY=0.377848
PRIMER_RIGHT_20_PENALTY=0.668315
PRIMER_LEFT_20_SEQUENCE=GACAATAATCGGGGTGGAAA
PRIMER_RIGHT_20_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_20=690,20
PRIMER_RIGHT_20=1545,20
PRIMER_LEFT_20_TM=59.622
PRIMER_RIGHT_20_TM=60.668
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=856
PRIMER_PAIR_21_PENALTY=1.049607
PRIMER_LEFT_21_PENALTY=0.124655
PRIMER_RIGHT_21_PENALTY=0.924951
PRIMER_LEFT_21_SEQUENCE=TAGAAGTGGCTTCGTCGGTT
PRIMER_RIGHT_21_SEQUENCE=GCAAGCAGCTGACATTTTCC
PRIMER_LEFT_21=885,20
PRIMER_RIGHT_21=1492,20
PRIMER_LEFT_21_TM=59.875
PRIMER_RIGHT_21_TM=60.925
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=608
PRIMER_PAIR_22_PENALTY=1.052513
PRIMER_LEFT_22_PENALTY=0.209870
PRIMER_RIGHT_22_PENALTY=0.842644
PRIMER_LEFT_22_SEQUENCE=CCACCCAAATCTGAACCAAC
PRIMER_RIGHT_22_SEQUENCE=TATCGAAACGACGAAACCAA
PRIMER_LEFT_22=652,20
PRIMER_RIGHT_22=1547,20
PRIMER_LEFT_22_TM=60.210
PRIMER_RIGHT_22_TM=59.157
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=896
PRIMER_PAIR_23_PENALTY=1.060485
PRIMER_LEFT_23_PENALTY=1.038144
PRIMER_RIGHT_23_PENALTY=0.022341
PRIMER_LEFT_23_SEQUENCE=GCAAAACTCCTCTCCTCGCT
PRIMER_RIGHT_23_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_23=924,20
PRIMER_RIGHT_23=1546,20
PRIMER_LEFT_23_TM=61.038
PRIMER_RIGHT_23_TM=59.978
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=623
PRIMER_PAIR_24_PENALTY=1.085108
PRIMER_LEFT_24_PENALTY=0.679391
PRIMER_RIGHT_24_PENALTY=0.405717
PRIMER_LEFT_24_SEQUENCE=CACTAACGGCTCCCACTTTG
PRIMER_RIGHT_24_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_24=828,20
PRIMER_RIGHT_24=1489,20
PRIMER_LEFT_24_TM=60.679
PRIMER_RIGHT_24_TM=60.406
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.3000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=662
PRIMER_PAIR_25_PENALTY=1.099372
PRIMER_LEFT_25_PENALTY=0.431057
PRIMER_RIGHT_25_PENALTY=0.668315
PRIMER_LEFT_25_SEQUENCE=ACGGCTCCCACTTTGAATAA
PRIMER_RIGHT_25_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_25=833,20
PRIMER_RIGHT_25=1545,20
PRIMER_LEFT_25_TM=59.569
PRIMER_RIGHT_25_TM=60.668
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.2000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=713
PRIMER_PAIR_26_PENALTY=1.100159
PRIMER_LEFT_26_PENALTY=1.077818
PRIMER_RIGHT_26_PENALTY=0.022341
PRIMER_LEFT_26_SEQUENCE=CTGTGAGGACACTAACGGCTC
PRIMER_RIGHT_26_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_26=819,21
PRIMER_RIGHT_26=1546,20
PRIMER_LEFT_26_TM=59.922
PRIMER_RIGHT_26_TM=59.978
PRIMER_LEFT_26_GC_PERCENT=57.143
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=728
PRIMER_PAIR_27_PENALTY=1.108359
PRIMER_LEFT_27_PENALTY=0.265715
PRIMER_RIGHT_27_PENALTY=0.842644
PRIMER_LEFT_27_SEQUENCE=ACTAACGGCTCCCACTTTGA
PRIMER_RIGHT_27_SEQUENCE=TATCGAAACGACGAAACCAA
PRIMER_LEFT_27=829,20
PRIMER_RIGHT_27=1547,20
PRIMER_LEFT_27_TM=59.734
PRIMER_RIGHT_27_TM=59.157
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.3000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=719
PRIMER_PAIR_28_PENALTY=1.112086
PRIMER_LEFT_28_PENALTY=1.089745
PRIMER_RIGHT_28_PENALTY=0.022341
PRIMER_LEFT_28_SEQUENCE=TGACAATAATCGGGGTGGAA
PRIMER_RIGHT_28_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_28=689,20
PRIMER_RIGHT_28=1546,20
PRIMER_LEFT_28_TM=61.090
PRIMER_RIGHT_28_TM=59.978
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=858
PRIMER_PAIR_29_PENALTY=1.112086
PRIMER_LEFT_29_PENALTY=1.089745
PRIMER_RIGHT_29_PENALTY=0.022341
PRIMER_LEFT_29_SEQUENCE=TTGACAATAATCGGGGTGGA
PRIMER_RIGHT_29_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_29=688,20
PRIMER_RIGHT_29=1546,20
PRIMER_LEFT_29_TM=61.090
PRIMER_RIGHT_29_TM=59.978
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=859
PRIMER_PAIR_30_PENALTY=1.134821
PRIMER_LEFT_30_PENALTY=0.209870
PRIMER_RIGHT_30_PENALTY=0.924951
PRIMER_LEFT_30_SEQUENCE=CCACCCAAATCTGAACCAAC
PRIMER_RIGHT_30_SEQUENCE=GCAAGCAGCTGACATTTTCC
PRIMER_LEFT_30=652,20
PRIMER_RIGHT_30=1492,20
PRIMER_LEFT_30_TM=60.210
PRIMER_RIGHT_30_TM=60.925
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=841
PRIMER_PAIR_31_PENALTY=1.140814
PRIMER_LEFT_31_PENALTY=1.118473
PRIMER_RIGHT_31_PENALTY=0.022341
PRIMER_LEFT_31_SEQUENCE=TGTGAGGACACTAACGGCTC
PRIMER_RIGHT_31_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_31=820,20
PRIMER_RIGHT_31=1546,20
PRIMER_LEFT_31_TM=58.882
PRIMER_RIGHT_31_TM=59.978
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=727
PRIMER_PAIR_32_PENALTY=1.161757
PRIMER_LEFT_32_PENALTY=1.139416
PRIMER_RIGHT_32_PENALTY=0.022341
PRIMER_LEFT_32_SEQUENCE=TTGGTCAAGTTGCAAAACTCC
PRIMER_RIGHT_32_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_32=913,21
PRIMER_RIGHT_32=1546,20
PRIMER_LEFT_32_TM=60.139
PRIMER_RIGHT_32_TM=59.978
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=634
PRIMER_PAIR_33_PENALTY=1.190667
PRIMER_LEFT_33_PENALTY=0.265715
PRIMER_RIGHT_33_PENALTY=0.924951
PRIMER_LEFT_33_SEQUENCE=ACTAACGGCTCCCACTTTGA
PRIMER_RIGHT_33_SEQUENCE=GCAAGCAGCTGACATTTTCC
PRIMER_LEFT_33=829,20
PRIMER_RIGHT_33=1492,20
PRIMER_LEFT_33_TM=59.734
PRIMER_RIGHT_33_TM=60.925
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=664
PRIMER_PAIR_34_PENALTY=1.193950
PRIMER_LEFT_34_PENALTY=0.788233
PRIMER_RIGHT_34_PENALTY=0.405717
PRIMER_LEFT_34_SEQUENCE=GACACTAACGGCTCCCACTT
PRIMER_RIGHT_34_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_34=826,20
PRIMER_RIGHT_34=1489,20
PRIMER_LEFT_34_TM=59.212
PRIMER_RIGHT_34_TM=60.406
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=664
PRIMER_PAIR_35_PENALTY=1.209949
PRIMER_LEFT_35_PENALTY=0.367306
PRIMER_RIGHT_35_PENALTY=0.842644
PRIMER_LEFT_35_SEQUENCE=CAATAATCGGGGTGGAAAAA
PRIMER_RIGHT_35_SEQUENCE=TATCGAAACGACGAAACCAA
PRIMER_LEFT_35=692,20
PRIMER_RIGHT_35=1547,20
PRIMER_LEFT_35_TM=59.633
PRIMER_RIGHT_35_TM=59.157
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.6000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=856
PRIMER_PAIR_36_PENALTY=1.220491
PRIMER_LEFT_36_PENALTY=0.377848
PRIMER_RIGHT_36_PENALTY=0.842644
PRIMER_LEFT_36_SEQUENCE=GACAATAATCGGGGTGGAAA
PRIMER_RIGHT_36_SEQUENCE=TATCGAAACGACGAAACCAA
PRIMER_LEFT_36=690,20
PRIMER_RIGHT_36=1547,20
PRIMER_LEFT_36_TM=59.622
PRIMER_RIGHT_36_TM=59.157
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=858
PRIMER_PAIR_37_PENALTY=1.220860
PRIMER_LEFT_37_PENALTY=0.209870
PRIMER_RIGHT_37_PENALTY=1.010990
PRIMER_LEFT_37_SEQUENCE=CCACCCAAATCTGAACCAAC
PRIMER_RIGHT_37_SEQUENCE=TATCGAAACGACGAAACCAAC
PRIMER_LEFT_37=652,20
PRIMER_RIGHT_37=1547,21
PRIMER_LEFT_37_TM=60.210
PRIMER_RIGHT_37_TM=59.989
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=2.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=896
PRIMER_PAIR_38_PENALTY=1.243653
PRIMER_LEFT_38_PENALTY=0.837936
PRIMER_RIGHT_38_PENALTY=0.405717
PRIMER_LEFT_38_SEQUENCE=CAAAACTCCTCTCCTCGCTC
PRIMER_RIGHT_38_SEQUENCE=AGCAGCTGACATTTTCCGAC
PRIMER_LEFT_38=925,20
PRIMER_RIGHT_38=1489,20
PRIMER_LEFT_38_TM=59.162
PRIMER_RIGHT_38_TM=60.406
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.9000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=565
PRIMER_PAIR_39_PENALTY=1.256683
PRIMER_LEFT_39_PENALTY=1.234342
PRIMER_RIGHT_39_PENALTY=0.022341
PRIMER_LEFT_39_SEQUENCE=TGGTCAAGTTGCAAAACTCCT
PRIMER_RIGHT_39_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_39=914,21
PRIMER_RIGHT_39=1546,20
PRIMER_LEFT_39_TM=59.766
PRIMER_RIGHT_39_TM=59.978
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=633
PRIMER_PAIR_40_PENALTY=1.273701
PRIMER_LEFT_40_PENALTY=0.431057
PRIMER_RIGHT_40_PENALTY=0.842644
PRIMER_LEFT_40_SEQUENCE=ACGGCTCCCACTTTGAATAA
PRIMER_RIGHT_40_SEQUENCE=TATCGAAACGACGAAACCAA
PRIMER_LEFT_40=833,20
PRIMER_RIGHT_40=1547,20
PRIMER_LEFT_40_TM=59.569
PRIMER_RIGHT_40_TM=59.157
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.2000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=715
PRIMER_PAIR_41_PENALTY=1.273812
PRIMER_LEFT_41_PENALTY=1.251471
PRIMER_RIGHT_41_PENALTY=0.022341
PRIMER_LEFT_41_SEQUENCE=TGGTCAAGTTGCAAAACTCC
PRIMER_RIGHT_41_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_41=914,20
PRIMER_RIGHT_41=1546,20
PRIMER_LEFT_41_TM=58.749
PRIMER_RIGHT_41_TM=59.978
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=633
PRIMER_PAIR_42_PENALTY=1.275805
PRIMER_LEFT_42_PENALTY=1.253464
PRIMER_RIGHT_42_PENALTY=0.022341
PRIMER_LEFT_42_SEQUENCE=TCTGATCTCACCGTCTTCACC
PRIMER_RIGHT_42_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_42=799,21
PRIMER_RIGHT_42=1546,20
PRIMER_LEFT_42_TM=60.253
PRIMER_RIGHT_42_TM=59.978
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=748
PRIMER_PAIR_43_PENALTY=1.276705
PRIMER_LEFT_43_PENALTY=0.265715
PRIMER_RIGHT_43_PENALTY=1.010990
PRIMER_LEFT_43_SEQUENCE=ACTAACGGCTCCCACTTTGA
PRIMER_RIGHT_43_SEQUENCE=TATCGAAACGACGAAACCAAC
PRIMER_LEFT_43=829,20
PRIMER_RIGHT_43=1547,21
PRIMER_LEFT_43_TM=59.734
PRIMER_RIGHT_43_TM=59.989
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=719
PRIMER_PAIR_44_PENALTY=1.278897
PRIMER_LEFT_44_PENALTY=0.610582
PRIMER_RIGHT_44_PENALTY=0.668315
PRIMER_LEFT_44_SEQUENCE=CACCCAAATCTGAACCAACA
PRIMER_RIGHT_44_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_44=653,20
PRIMER_RIGHT_44=1545,20
PRIMER_LEFT_44_TM=59.389
PRIMER_RIGHT_44_TM=60.668
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=2.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=893
PRIMER_PAIR_45_PENALTY=1.284463
PRIMER_LEFT_45_PENALTY=0.616149
PRIMER_RIGHT_45_PENALTY=0.668315
PRIMER_LEFT_45_SEQUENCE=TCAAACACAACACAGGCCAC
PRIMER_RIGHT_45_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_45=636,20
PRIMER_RIGHT_45=1545,20
PRIMER_LEFT_45_TM=60.616
PRIMER_RIGHT_45_TM=60.668
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=910
PRIMER_PAIR_46_PENALTY=1.301593
PRIMER_LEFT_46_PENALTY=1.279252
PRIMER_RIGHT_46_PENALTY=0.022341
PRIMER_LEFT_46_SEQUENCE=ACACTAACGGCTCCCACTTT
PRIMER_RIGHT_46_SEQUENCE=ATCGAAACGACGAAACCAAC
PRIMER_LEFT_46=827,20
PRIMER_RIGHT_46=1546,20
PRIMER_LEFT_46_TM=58.721
PRIMER_RIGHT_46_TM=59.978
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=720
PRIMER_PAIR_47_PENALTY=1.347706
PRIMER_LEFT_47_PENALTY=0.679391
PRIMER_RIGHT_47_PENALTY=0.668315
PRIMER_LEFT_47_SEQUENCE=CACTAACGGCTCCCACTTTG
PRIMER_RIGHT_47_SEQUENCE=TCGAAACGACGAAACCAACT
PRIMER_LEFT_47=828,20
PRIMER_RIGHT_47=1545,20
PRIMER_LEFT_47_TM=60.679
PRIMER_RIGHT_47_TM=60.668
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.3000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=718
PRIMER_PAIR_48_PENALTY=1.356009
PRIMER_LEFT_48_PENALTY=0.431057
PRIMER_RIGHT_48_PENALTY=0.924951
PRIMER_LEFT_48_SEQUENCE=ACGGCTCCCACTTTGAATAA
PRIMER_RIGHT_48_SEQUENCE=GCAAGCAGCTGACATTTTCC
PRIMER_LEFT_48=833,20
PRIMER_RIGHT_48=1492,20
PRIMER_LEFT_48_TM=59.569
PRIMER_RIGHT_48_TM=60.925
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.2000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=660
PRIMER_PAIR_49_PENALTY=1.361376
PRIMER_LEFT_49_PENALTY=0.209870
PRIMER_RIGHT_49_PENALTY=1.151506
PRIMER_LEFT_49_SEQUENCE=CCACCCAAATCTGAACCAAC
PRIMER_RIGHT_49_SEQUENCE=CGAAACGACGAAACCAACTAA
PRIMER_LEFT_49=652,20
PRIMER_RIGHT_49=1544,21
PRIMER_LEFT_49_TM=60.210
PRIMER_RIGHT_49_TM=60.152
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=5.7000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=893
=
SEQUENCE_ID=MAGIC.329_2_6272181_6272236_wil-2_zu-0_23812_285482_TRUE
SEQUENCE_TARGET=950,155
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCGACATTGCNNNATATCTCANGCNNCGCATTTGTNAAGTAATCATTGTAAATGAAANNCANTAAGNTCTCTGCTNCTTTNTNACNANGATTTTTNCGTTGAGTTNAAANAGAATTCAANATGTCGAANTNNNGTAAACNTTTTACCATTGTNCATGTCTTGAATGTGGCATCAGAATATTCNATCTTGNTATTAGTTCCAAATNGAAGATTTTATCGTTGATAACNCANGAAGTGTCATACATTNTTTCACCCATAGCANNNNNNNNNTTTCTCTCAAGTATTCCCNTCTTTGGGTCAAANANNGTGCAATGAGTGNCATNGTTGAGGATGAAAANNATCNTTCAAATAAAAANCAAAGNTTNGCAAAGCTAATCNGTCNAANGTNTTTTGAATNATTATCTCCNAATTTNATCCAAAGATTNCTNTTTTTTATACCANNNNNNNNNNNNNNNNNNNNNNNNCTCTTTCAACTGNAGTNTTAACTNNGTAATCTCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTAATTCTGATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTNNNNNNNNNCTNCAATGTTNGACCNACCANATTTTTTGTGNNNTACAAAGNAATAACCAATCTTAGTNCACTTCAANCNACCTTNTTGCGGANNNNGTAAATNTTCATGAATCTCATTCGTATNNNNTTCGTGGNGTTCNTAACGGCACCGTNTTANNCNGNATTGAGTANNNNNNTTCTTCTAATAGATTTAATTGGGCTAATGGATCGGCCCACTTCGNCCNACGGGNATGAATATATATGATCCTANTAAAGCCAGNAAGGACACNNAGCCAANTTTNNNNNAATCTAAAGATGGGGAAANCGAAGCGATACTGGTTACTNAAAACNGAGCCAANTGAGTGGTCATGGTCAGACCAAGAATCNAACGGCGGNATCAGCAAATGGGACGGCGTCAAGAACAAACAAGCCCAGAANAATCTTAAANCCATGACTTTAGGAGATCTCTGTTTCTTCTACCATTCAGGNACCAAANCNAGATNCGTCGTTGGNGTGGTTGAAGTATCACNTGAATGGTACACTGNTGATGNTGAAGGAGTAGAAGGAGAAGGAGCGGTTGATGTNAAAGCGATTGGAGAAATGAGGAAATGTGTGGATTTGAAGGAAATGAAAGGAGACAAAGGGATTANTACTAANGGTTTTGTTTTGTTTAGACAGCCGAGGTTGTCTGTTGTNCCTGTTGANGAAGATGTTTGGAATANGATTTGTGAATTGGGAAATGGGTTTTGTGGAGATGGTAAGGAAGATTGTGAAAGTAGTGATGNNNNATGANNNNNNNNNNNNNNNNNNNNNNNGTNTNATGTGATTNAAAGNTNTNAACTTNGTNAGGNTTTTGNGGNNTTTGCTTAATGATTGATTCGTAGTGGTGATGCTNTTGNGTGGTGCATTTTGTCGAACATGTGGTGTGCAAGAAACTATGTTATATTTTGTGAGATTTAATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=7
PRIMER_RIGHT_NUM_RETURNED=7
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=7
PRIMER_PAIR_0_PENALTY=3.537562
PRIMER_LEFT_0_PENALTY=1.813616
PRIMER_RIGHT_0_PENALTY=1.723946
PRIMER_LEFT_0_SEQUENCE=TGTCTTGAATGTGGCATCAG
PRIMER_RIGHT_0_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_0=329,20
PRIMER_RIGHT_0=1249,20
PRIMER_LEFT_0_TM=58.186
PRIMER_RIGHT_0_TM=61.724
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=5.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=921
PRIMER_PAIR_1_PENALTY=4.012350
PRIMER_LEFT_1_PENALTY=2.288404
PRIMER_RIGHT_1_PENALTY=1.723946
PRIMER_LEFT_1_SEQUENCE=GTCTTGAATGTGGCATCAGAA
PRIMER_RIGHT_1_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_1=330,21
PRIMER_RIGHT_1=1249,20
PRIMER_LEFT_1_TM=58.712
PRIMER_RIGHT_1_TM=61.724
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=920
PRIMER_PAIR_2_PENALTY=4.139334
PRIMER_LEFT_2_PENALTY=2.415388
PRIMER_RIGHT_2_PENALTY=1.723946
PRIMER_LEFT_2_SEQUENCE=ATGTCTTGAATGTGGCATCAG
PRIMER_RIGHT_2_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_2=328,21
PRIMER_RIGHT_2=1249,20
PRIMER_LEFT_2_TM=58.585
PRIMER_RIGHT_2_TM=61.724
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=922
PRIMER_PAIR_3_PENALTY=4.285401
PRIMER_LEFT_3_PENALTY=2.561455
PRIMER_RIGHT_3_PENALTY=1.723946
PRIMER_LEFT_3_SEQUENCE=CATGTCTTGAATGTGGCATC
PRIMER_RIGHT_3_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_3=327,20
PRIMER_RIGHT_3=1249,20
PRIMER_LEFT_3_TM=57.439
PRIMER_RIGHT_3_TM=61.724
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=923
PRIMER_PAIR_4_PENALTY=5.217554
PRIMER_LEFT_4_PENALTY=3.493609
PRIMER_RIGHT_4_PENALTY=1.723946
PRIMER_LEFT_4_SEQUENCE=CATGTCTTGAATGTGGCATCAG
PRIMER_RIGHT_4_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_4=327,22
PRIMER_RIGHT_4=1249,20
PRIMER_LEFT_4_TM=61.494
PRIMER_RIGHT_4_TM=61.724
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=923
PRIMER_PAIR_5_PENALTY=5.335947
PRIMER_LEFT_5_PENALTY=3.612001
PRIMER_RIGHT_5_PENALTY=1.723946
PRIMER_LEFT_5_SEQUENCE=TGTCTTGAATGTGGCATCAGAA
PRIMER_RIGHT_5_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_5=329,22
PRIMER_RIGHT_5=1249,20
PRIMER_LEFT_5_TM=61.612
PRIMER_RIGHT_5_TM=61.724
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=5.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=921
PRIMER_PAIR_6_PENALTY=10.104730
PRIMER_LEFT_6_PENALTY=8.380785
PRIMER_RIGHT_6_PENALTY=1.723946
PRIMER_LEFT_6_SEQUENCE=CATGTCTTGAATGTGGCATCAGAA
PRIMER_RIGHT_6_SEQUENCE=GAAGTGGGCCGATCCATTAG
PRIMER_LEFT_6=327,24
PRIMER_RIGHT_6=1249,20
PRIMER_LEFT_6_TM=64.381
PRIMER_RIGHT_6_TM=61.724
PRIMER_LEFT_6_GC_PERCENT=41.667
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=5.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.433_1_17579775_17579875_ct-1_hi-0_842050_147630_TRUE
SEQUENCE_TARGET=950,200
SEQUENCE_TEMPLATE=TTNCACTAGGGTNACAGAAANATGTAACAAAACTCTCACTAATGCAAAACAGAACAAGTGTGGTNTAGTAGTTCTAAAGAAGCAGNACGAACATTGTAAATGTCGTTAAATGTAAAGACAAGTGGTGTAGAATANTNGTANTTTAGTGCTGGGNATGATGACTATGTNACTTTATTTTGTGTTAGAGAAGAGNCACTTGCGGATTTACTTCAGCACGACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNATTACGANTAAGGTTGAGGCCAAAATGNTGTTATGGAGANNAAATTTANAANAGAAAATACTTANAAACNAATTATACTGTTTTTNTTNAGTTGACATCANGCTTTNATTATAANAAAACGNATATGAGTAAGAANCAGNTTAATANNNNNNNNNTANNATTTNATTTNANNGTCGAATATAATAANANTGTGATCCTTGGCATTTGTATCCAAAAGCGCGCNGTAATGNTTTNTTATTCTTCATAACCATGATCAGANNAACNNATGGGTANACNTNCTCNNNNNNNNNAATNATAATCAACTGTTACTGNCANATCAAACGAATATNTNTTCTCCCATGTCATTTCAGANTNGTCNNNNTAATGNNCATATCTNTTNTNTNGANCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTGGTTTACANNNCGTTTAAACTNCTCTNGCATGTATGTNATTCTAANTCAAAAGCGGGAAACAAATTTTGAANACTCNGNGNCATAANCGTAAATTANNATGNTGATTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACTACTCTATTTCGCAACGTTAACTCTTCCCCTTCCACAAGTAACGGTTAGCGAANGTTGAANTTTCAAAATTCATTTNTTCAAATCCTCTTTCAGAAAATCGCGTTNGGGTTTACATAATCGCATGGAGAAGCGANTTTTCATNTTCTATCTTCTTCNGATTCTTNNCTNCGATCAATTGAATTGCGTCTCTGATTCNTTNGGTCAATTCAAANNGGGATCAAAGGGTTTTGATTTTGGGTCGNTGATNATCTGACGATTGATGCNAGGATTATTACTTGTCTATTGATTTGAGTCCTGATCGTGTGATAGTCCTACGAAATCGAAGAAGGTACGAAATGTTTCGATCTCTCTCTNTTCTTGGTTAATTTGGGAATCTAGGGTTTGGATTTGGAATTTNCTTTCGTTGATTTCTGTTGGAATTTAGAGGTTTGATNGTTCTGGGATCTTTCTATTAGGGTAATGTTTAACTTGTTCGATAGGTTTNAAATTTGGTGATCGGNTAACAGTTGAACAGAGTCTACATCAAAATTTCTCTCTTACNNNNNNNNNNGATGAGTCTAGATGGTNAAAAAAANCCAACGATTTAGANAATTTCCCTCANGTCCATGATTTTGATTGTACN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4692167_4692194_edi-0_wil-2_55687_20406_TRUE
SEQUENCE_TARGET=950,127
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGAATATACCTGNATATTNTGCAGNGGAANTATGAANCATGNTAATTCGNNNNTGGTTTAGNANTCCAAGNATTGTGCCTCTACTGGTANCCACACGCATNCAAANTNGATCAANCCCNNAATGCTANTTCCTTTGAGATCTAATCTCAAAACTTAGAAAAACAATNTTTCTCTTTGTTTTTATGNGTTGAAGATAATTTATCAAATTTTNAGAGAACATNACCGTAATAACNGTAACCGTGGTCATGCCTANNTNTNNNNNNTATTNATCATNCCAAAATAGACATACCATCATCAGTCGTTAAAACAANTTGANAANTTATCNNCGACTTTCTTTCTCTTCTCTCTCNTAATCCTTNAGAGAGAGAATGTCTCCGATGGTTGTTTTTGGTGGCAACGACATGTTTCTTCACTTGCGTTGGCTCTCATATGCCCAACACANTTGGTTACCTCAACGACGGTGACTGGTTTTCTCGTCCGNTTTNGGTGGATTCATCTCCGCCGTTGGNNGGCTCCCGTCCGGAATTGTCTGGCTCACATAGTAGAGNTGTCCGGCTTTCGTCCAGCACCGTCGCGTTTCCACGGTGGCATGTGGCTAGCTTTCTCCTATGCATCCTCTTTCCTTTGTTTATCTGTGTTTTTACTTTTGTTTGCNTTTAATAATCTCACATACATGGGTTGGTAATGACANTGTAAGTAATCTAGCTTTNTACTGTTNTGTTCCGATCTATTTCATGNTGAGACTTAATTGCAGAAATNAAATAAGAGATCTNNGAATGANGATGAAGATAAGAAGTGGAATCCGTCGCTTATTAATCATTGCCAATCTGCTGATGTATCTTTAATTGGAGCCTCTTTACTNTCTACCGGTGAGAAANCCTTAAGAATCCATGTTAGATAGTCCTATCANCANTGATTCTGTCACCGGAANTGAAGCTCCNACGGTGGAGGTTGAAGTCGGTTTGCTTAAGATTGCTCATCTGACACGTGGTTATGTCGACGTGTGTTTCAGGATTGAGTCAAATCCAGTTTTCTTCTTCTGTGAGCAAGATGAAATCTTCTGATGGGCCTANNNNNNCNANNAAACATTTTGTAATCNTAAGGGNTCAAAAACGGNTGCATCTCAAAAGTCATAATGCATCACTTTTATAATAGGTTCACNNAACCTAATGCATATAAAGTCAATGGGCCAAGCCCAATCCATGCAANGTNACTTTCTNTACNAGAATAAACCACATATTCGTATTAGTGCACATATGTATATTTTATATACATATATAACATTATTTATTTAAGTGCTCAAATACAAATATAAGTATATCTATATATNNCATATATATTATATACTTTTACTTTAATTTATATATNATATATATANANNNNNAATCTTTTAACCTAATATTAGTTTAATCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAACTCATAGATTATAAGCGNTCTCTAGCAAAACATTATAACCATCTAATNNNNNNNNNNNNNNNNNNNNNNNNNNTANAACTTTAACTAGAGTNNAGTACAAACGATTTCAAGATATTCCCAACATTATTTAGNTTTGATGATGTGTACAATGTCTNGAGTNNTGTGTGTGTGGATTGGTAGANNNNNNNNNNNNNNNTTCCCTGATAGTTTTTCCCCTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.332534
PRIMER_LEFT_0_PENALTY=0.119028
PRIMER_RIGHT_0_PENALTY=0.213506
PRIMER_LEFT_0_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_0_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_0=712,20
PRIMER_RIGHT_0=1121,20
PRIMER_LEFT_0_TM=59.881
PRIMER_RIGHT_0_TM=60.214
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=410
PRIMER_PAIR_1_PENALTY=0.383555
PRIMER_LEFT_1_PENALTY=0.170049
PRIMER_RIGHT_1_PENALTY=0.213506
PRIMER_LEFT_1_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_1_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_1=682,20
PRIMER_RIGHT_1=1121,20
PRIMER_LEFT_1_TM=59.830
PRIMER_RIGHT_1_TM=60.214
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=440
PRIMER_PAIR_2_PENALTY=0.516061
PRIMER_LEFT_2_PENALTY=0.302555
PRIMER_RIGHT_2_PENALTY=0.213506
PRIMER_LEFT_2_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_2_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_2=702,20
PRIMER_RIGHT_2=1121,20
PRIMER_LEFT_2_TM=59.697
PRIMER_RIGHT_2_TM=60.214
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=420
PRIMER_PAIR_3_PENALTY=0.786154
PRIMER_LEFT_3_PENALTY=0.119028
PRIMER_RIGHT_3_PENALTY=0.667126
PRIMER_LEFT_3_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_3_SEQUENCE=AGCAAACCGACTTCAACCTC
PRIMER_LEFT_3=712,20
PRIMER_RIGHT_3=1276,20
PRIMER_LEFT_3_TM=59.881
PRIMER_RIGHT_3_TM=59.333
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=565
PRIMER_PAIR_4_PENALTY=0.969681
PRIMER_LEFT_4_PENALTY=0.302555
PRIMER_RIGHT_4_PENALTY=0.667126
PRIMER_LEFT_4_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_4_SEQUENCE=AGCAAACCGACTTCAACCTC
PRIMER_LEFT_4=702,20
PRIMER_RIGHT_4=1276,20
PRIMER_LEFT_4_TM=59.697
PRIMER_RIGHT_4_TM=59.333
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=575
PRIMER_PAIR_5_PENALTY=0.988110
PRIMER_LEFT_5_PENALTY=0.320985
PRIMER_RIGHT_5_PENALTY=0.667126
PRIMER_LEFT_5_SEQUENCE=GCTCTCATATGCCCAACACA
PRIMER_RIGHT_5_SEQUENCE=AGCAAACCGACTTCAACCTC
PRIMER_LEFT_5=731,20
PRIMER_RIGHT_5=1276,20
PRIMER_LEFT_5_TM=59.679
PRIMER_RIGHT_5_TM=59.333
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=546
PRIMER_PAIR_6_PENALTY=1.076557
PRIMER_LEFT_6_PENALTY=0.863051
PRIMER_RIGHT_6_PENALTY=0.213506
PRIMER_LEFT_6_SEQUENCE=TCTCCGATGGTTGTTTTTGG
PRIMER_RIGHT_6_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_6=681,20
PRIMER_RIGHT_6=1121,20
PRIMER_LEFT_6_TM=60.863
PRIMER_RIGHT_6_TM=60.214
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.8000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=441
PRIMER_PAIR_7_PENALTY=1.079173
PRIMER_LEFT_7_PENALTY=0.119028
PRIMER_RIGHT_7_PENALTY=0.960145
PRIMER_LEFT_7_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_7_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_7=712,20
PRIMER_RIGHT_7=1273,20
PRIMER_LEFT_7_TM=59.881
PRIMER_RIGHT_7_TM=59.040
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=562
PRIMER_PAIR_8_PENALTY=1.112447
PRIMER_LEFT_8_PENALTY=0.445321
PRIMER_RIGHT_8_PENALTY=0.667126
PRIMER_LEFT_8_SEQUENCE=GGTGACTGGTTTTCTCGTCC
PRIMER_RIGHT_8_SEQUENCE=AGCAAACCGACTTCAACCTC
PRIMER_LEFT_8=769,20
PRIMER_RIGHT_8=1276,20
PRIMER_LEFT_8_TM=59.555
PRIMER_RIGHT_8_TM=59.333
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=508
PRIMER_PAIR_9_PENALTY=1.130194
PRIMER_LEFT_9_PENALTY=0.170049
PRIMER_RIGHT_9_PENALTY=0.960145
PRIMER_LEFT_9_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_9_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_9=682,20
PRIMER_RIGHT_9=1273,20
PRIMER_LEFT_9_TM=59.830
PRIMER_RIGHT_9_TM=59.040
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=592
PRIMER_PAIR_10_PENALTY=1.139564
PRIMER_LEFT_10_PENALTY=0.119028
PRIMER_RIGHT_10_PENALTY=1.020535
PRIMER_LEFT_10_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_10_SEQUENCE=AATAAGCGACGGATTCCACTT
PRIMER_LEFT_10=712,20
PRIMER_RIGHT_10=1123,21
PRIMER_LEFT_10_TM=59.881
PRIMER_RIGHT_10_TM=59.979
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=412
PRIMER_PAIR_11_PENALTY=1.157285
PRIMER_LEFT_11_PENALTY=0.943780
PRIMER_RIGHT_11_PENALTY=0.213506
PRIMER_LEFT_11_SEQUENCE=TTTCTTCACTTGCGTTGGCT
PRIMER_RIGHT_11_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_11=714,20
PRIMER_RIGHT_11=1121,20
PRIMER_LEFT_11_TM=60.944
PRIMER_RIGHT_11_TM=60.214
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=408
PRIMER_PAIR_12_PENALTY=1.158971
PRIMER_LEFT_12_PENALTY=0.491845
PRIMER_RIGHT_12_PENALTY=0.667126
PRIMER_LEFT_12_SEQUENCE=GGCTCTCATATGCCCAACAC
PRIMER_RIGHT_12_SEQUENCE=AGCAAACCGACTTCAACCTC
PRIMER_LEFT_12=730,20
PRIMER_RIGHT_12=1276,20
PRIMER_LEFT_12_TM=60.492
PRIMER_RIGHT_12_TM=59.333
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=547
PRIMER_PAIR_13_PENALTY=1.182854
PRIMER_LEFT_13_PENALTY=0.119028
PRIMER_RIGHT_13_PENALTY=1.063825
PRIMER_LEFT_13_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_13_SEQUENCE=CAAACCGACTTCAACCTCCA
PRIMER_LEFT_13=712,20
PRIMER_RIGHT_13=1274,20
PRIMER_LEFT_13_TM=59.881
PRIMER_RIGHT_13_TM=61.064
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=563
PRIMER_PAIR_14_PENALTY=1.190584
PRIMER_LEFT_14_PENALTY=0.170049
PRIMER_RIGHT_14_PENALTY=1.020535
PRIMER_LEFT_14_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_14_SEQUENCE=AATAAGCGACGGATTCCACTT
PRIMER_LEFT_14=682,20
PRIMER_RIGHT_14=1123,21
PRIMER_LEFT_14_TM=59.830
PRIMER_RIGHT_14_TM=59.979
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=442
PRIMER_PAIR_15_PENALTY=1.233874
PRIMER_LEFT_15_PENALTY=0.170049
PRIMER_RIGHT_15_PENALTY=1.063825
PRIMER_LEFT_15_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_15_SEQUENCE=CAAACCGACTTCAACCTCCA
PRIMER_LEFT_15=682,20
PRIMER_RIGHT_15=1274,20
PRIMER_LEFT_15_TM=59.830
PRIMER_RIGHT_15_TM=61.064
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=593
PRIMER_PAIR_16_PENALTY=1.262700
PRIMER_LEFT_16_PENALTY=0.302555
PRIMER_RIGHT_16_PENALTY=0.960145
PRIMER_LEFT_16_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_16_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_16=702,20
PRIMER_RIGHT_16=1273,20
PRIMER_LEFT_16_TM=59.697
PRIMER_RIGHT_16_TM=59.040
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=572
PRIMER_PAIR_17_PENALTY=1.272377
PRIMER_LEFT_17_PENALTY=0.119028
PRIMER_RIGHT_17_PENALTY=1.153349
PRIMER_LEFT_17_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_17_SEQUENCE=AGCGACGGATTCCACTTCTT
PRIMER_LEFT_17=712,20
PRIMER_RIGHT_17=1119,20
PRIMER_LEFT_17_TM=59.881
PRIMER_RIGHT_17_TM=61.153
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=408
PRIMER_PAIR_18_PENALTY=1.272377
PRIMER_LEFT_18_PENALTY=0.119028
PRIMER_RIGHT_18_PENALTY=1.153349
PRIMER_LEFT_18_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_18_SEQUENCE=AAGCGACGGATTCCACTTCT
PRIMER_LEFT_18=712,20
PRIMER_RIGHT_18=1120,20
PRIMER_LEFT_18_TM=59.881
PRIMER_RIGHT_18_TM=61.153
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=409
PRIMER_PAIR_19_PENALTY=1.279274
PRIMER_LEFT_19_PENALTY=0.119028
PRIMER_RIGHT_19_PENALTY=1.160245
PRIMER_LEFT_19_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_19_SEQUENCE=AAGCAAACCGACTTCAACCT
PRIMER_LEFT_19=712,20
PRIMER_RIGHT_19=1277,20
PRIMER_LEFT_19_TM=59.881
PRIMER_RIGHT_19_TM=58.840
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=566
PRIMER_PAIR_20_PENALTY=1.281129
PRIMER_LEFT_20_PENALTY=0.320985
PRIMER_RIGHT_20_PENALTY=0.960145
PRIMER_LEFT_20_SEQUENCE=GCTCTCATATGCCCAACACA
PRIMER_RIGHT_20_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_20=731,20
PRIMER_RIGHT_20=1273,20
PRIMER_LEFT_20_TM=59.679
PRIMER_RIGHT_20_TM=59.040
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=543
PRIMER_PAIR_21_PENALTY=1.323090
PRIMER_LEFT_21_PENALTY=0.302555
PRIMER_RIGHT_21_PENALTY=1.020535
PRIMER_LEFT_21_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_21_SEQUENCE=AATAAGCGACGGATTCCACTT
PRIMER_LEFT_21=702,20
PRIMER_RIGHT_21=1123,21
PRIMER_LEFT_21_TM=59.697
PRIMER_RIGHT_21_TM=59.979
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=422
PRIMER_PAIR_22_PENALTY=1.323398
PRIMER_LEFT_22_PENALTY=0.170049
PRIMER_RIGHT_22_PENALTY=1.153349
PRIMER_LEFT_22_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_22_SEQUENCE=AGCGACGGATTCCACTTCTT
PRIMER_LEFT_22=682,20
PRIMER_RIGHT_22=1119,20
PRIMER_LEFT_22_TM=59.830
PRIMER_RIGHT_22_TM=61.153
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=438
PRIMER_PAIR_23_PENALTY=1.323398
PRIMER_LEFT_23_PENALTY=0.170049
PRIMER_RIGHT_23_PENALTY=1.153349
PRIMER_LEFT_23_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_23_SEQUENCE=AAGCGACGGATTCCACTTCT
PRIMER_LEFT_23=682,20
PRIMER_RIGHT_23=1120,20
PRIMER_LEFT_23_TM=59.830
PRIMER_RIGHT_23_TM=61.153
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=439
PRIMER_PAIR_24_PENALTY=1.329471
PRIMER_LEFT_24_PENALTY=1.115965
PRIMER_RIGHT_24_PENALTY=0.213506
PRIMER_LEFT_24_SEQUENCE=CTTCACTTGCGTTGGCTCTC
PRIMER_RIGHT_24_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_24=717,20
PRIMER_RIGHT_24=1121,20
PRIMER_LEFT_24_TM=61.116
PRIMER_RIGHT_24_TM=60.214
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=405
PRIMER_PAIR_25_PENALTY=1.329471
PRIMER_LEFT_25_PENALTY=1.115965
PRIMER_RIGHT_25_PENALTY=0.213506
PRIMER_LEFT_25_SEQUENCE=TCTTCACTTGCGTTGGCTCT
PRIMER_RIGHT_25_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_25=716,20
PRIMER_RIGHT_25=1121,20
PRIMER_LEFT_25_TM=61.116
PRIMER_RIGHT_25_TM=60.214
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=406
PRIMER_PAIR_26_PENALTY=1.330384
PRIMER_LEFT_26_PENALTY=0.012470
PRIMER_RIGHT_26_PENALTY=1.317914
PRIMER_LEFT_26_SEQUENCE=AACGACGGTGACTGGTTTTC
PRIMER_RIGHT_26_SEQUENCE=AACCGACTTCAACCTCCACC
PRIMER_LEFT_26=763,20
PRIMER_RIGHT_26=1272,20
PRIMER_LEFT_26_TM=60.012
PRIMER_RIGHT_26_TM=61.318
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=9.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=510
PRIMER_PAIR_27_PENALTY=1.337977
PRIMER_LEFT_27_PENALTY=0.377832
PRIMER_RIGHT_27_PENALTY=0.960145
PRIMER_LEFT_27_SEQUENCE=ACGGTGACTGGTTTTCTCGT
PRIMER_RIGHT_27_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_27=767,20
PRIMER_RIGHT_27=1273,20
PRIMER_LEFT_27_TM=59.622
PRIMER_RIGHT_27_TM=59.040
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=507
PRIMER_PAIR_28_PENALTY=1.337977
PRIMER_LEFT_28_PENALTY=0.377832
PRIMER_RIGHT_28_PENALTY=0.960145
PRIMER_LEFT_28_SEQUENCE=ACGACGGTGACTGGTTTTCT
PRIMER_RIGHT_28_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_28=764,20
PRIMER_RIGHT_28=1273,20
PRIMER_LEFT_28_TM=59.622
PRIMER_RIGHT_28_TM=59.040
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=510
PRIMER_PAIR_29_PENALTY=1.366381
PRIMER_LEFT_29_PENALTY=0.302555
PRIMER_RIGHT_29_PENALTY=1.063825
PRIMER_LEFT_29_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_29_SEQUENCE=CAAACCGACTTCAACCTCCA
PRIMER_LEFT_29=702,20
PRIMER_RIGHT_29=1274,20
PRIMER_LEFT_29_TM=59.697
PRIMER_RIGHT_29_TM=61.064
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=573
PRIMER_PAIR_30_PENALTY=1.374596
PRIMER_LEFT_30_PENALTY=1.161090
PRIMER_RIGHT_30_PENALTY=0.213506
PRIMER_LEFT_30_SEQUENCE=ATGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_30_SEQUENCE=TAAGCGACGGATTCCACTTC
PRIMER_LEFT_30=711,21
PRIMER_RIGHT_30=1121,20
PRIMER_LEFT_30_TM=60.161
PRIMER_RIGHT_30_TM=60.214
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=411
PRIMER_PAIR_31_PENALTY=1.384810
PRIMER_LEFT_31_PENALTY=0.320985
PRIMER_RIGHT_31_PENALTY=1.063825
PRIMER_LEFT_31_SEQUENCE=GCTCTCATATGCCCAACACA
PRIMER_RIGHT_31_SEQUENCE=CAAACCGACTTCAACCTCCA
PRIMER_LEFT_31=731,20
PRIMER_RIGHT_31=1274,20
PRIMER_LEFT_31_TM=59.679
PRIMER_RIGHT_31_TM=61.064
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=544
PRIMER_PAIR_32_PENALTY=1.405466
PRIMER_LEFT_32_PENALTY=0.445321
PRIMER_RIGHT_32_PENALTY=0.960145
PRIMER_LEFT_32_SEQUENCE=GGTGACTGGTTTTCTCGTCC
PRIMER_RIGHT_32_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_32=769,20
PRIMER_RIGHT_32=1273,20
PRIMER_LEFT_32_TM=59.555
PRIMER_RIGHT_32_TM=59.040
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.6000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=505
PRIMER_PAIR_33_PENALTY=1.436942
PRIMER_LEFT_33_PENALTY=0.119028
PRIMER_RIGHT_33_PENALTY=1.317914
PRIMER_LEFT_33_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_33_SEQUENCE=AACCGACTTCAACCTCCACC
PRIMER_LEFT_33=712,20
PRIMER_RIGHT_33=1272,20
PRIMER_LEFT_33_TM=59.881
PRIMER_RIGHT_33_TM=61.318
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=561
PRIMER_PAIR_34_PENALTY=1.441658
PRIMER_LEFT_34_PENALTY=0.377832
PRIMER_RIGHT_34_PENALTY=1.063825
PRIMER_LEFT_34_SEQUENCE=ACGGTGACTGGTTTTCTCGT
PRIMER_RIGHT_34_SEQUENCE=CAAACCGACTTCAACCTCCA
PRIMER_LEFT_34=767,20
PRIMER_RIGHT_34=1274,20
PRIMER_LEFT_34_TM=59.622
PRIMER_RIGHT_34_TM=61.064
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=508
PRIMER_PAIR_35_PENALTY=1.446642
PRIMER_LEFT_35_PENALTY=0.119028
PRIMER_RIGHT_35_PENALTY=1.327613
PRIMER_LEFT_35_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_35_SEQUENCE=ATAAGCGACGGATTCCACTT
PRIMER_LEFT_35=712,20
PRIMER_RIGHT_35=1122,20
PRIMER_LEFT_35_TM=59.881
PRIMER_RIGHT_35_TM=58.672
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=411
PRIMER_PAIR_36_PENALTY=1.446642
PRIMER_LEFT_36_PENALTY=0.119028
PRIMER_RIGHT_36_PENALTY=1.327613
PRIMER_LEFT_36_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_36_SEQUENCE=AATAAGCGACGGATTCCACT
PRIMER_LEFT_36=712,20
PRIMER_RIGHT_36=1123,20
PRIMER_LEFT_36_TM=59.881
PRIMER_RIGHT_36_TM=58.672
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=412
PRIMER_PAIR_37_PENALTY=1.451990
PRIMER_LEFT_37_PENALTY=0.491845
PRIMER_RIGHT_37_PENALTY=0.960145
PRIMER_LEFT_37_SEQUENCE=GGCTCTCATATGCCCAACAC
PRIMER_RIGHT_37_SEQUENCE=AAACCGACTTCAACCTCCAC
PRIMER_LEFT_37=730,20
PRIMER_RIGHT_37=1273,20
PRIMER_LEFT_37_TM=60.492
PRIMER_RIGHT_37_TM=59.040
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=2.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=544
PRIMER_PAIR_38_PENALTY=1.455904
PRIMER_LEFT_38_PENALTY=0.302555
PRIMER_RIGHT_38_PENALTY=1.153349
PRIMER_LEFT_38_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_38_SEQUENCE=AGCGACGGATTCCACTTCTT
PRIMER_LEFT_38=702,20
PRIMER_RIGHT_38=1119,20
PRIMER_LEFT_38_TM=59.697
PRIMER_RIGHT_38_TM=61.153
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=2.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=418
PRIMER_PAIR_39_PENALTY=1.455904
PRIMER_LEFT_39_PENALTY=0.302555
PRIMER_RIGHT_39_PENALTY=1.153349
PRIMER_LEFT_39_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_39_SEQUENCE=AAGCGACGGATTCCACTTCT
PRIMER_LEFT_39=702,20
PRIMER_RIGHT_39=1120,20
PRIMER_LEFT_39_TM=59.697
PRIMER_RIGHT_39_TM=61.153
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=419
PRIMER_PAIR_40_PENALTY=1.462800
PRIMER_LEFT_40_PENALTY=0.302555
PRIMER_RIGHT_40_PENALTY=1.160245
PRIMER_LEFT_40_SEQUENCE=GGCAACGACATGTTTCTTCA
PRIMER_RIGHT_40_SEQUENCE=AAGCAAACCGACTTCAACCT
PRIMER_LEFT_40=702,20
PRIMER_RIGHT_40=1277,20
PRIMER_LEFT_40_TM=59.697
PRIMER_RIGHT_40_TM=58.840
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=576
PRIMER_PAIR_41_PENALTY=1.481230
PRIMER_LEFT_41_PENALTY=0.320985
PRIMER_RIGHT_41_PENALTY=1.160245
PRIMER_LEFT_41_SEQUENCE=GCTCTCATATGCCCAACACA
PRIMER_RIGHT_41_SEQUENCE=AAGCAAACCGACTTCAACCT
PRIMER_LEFT_41=731,20
PRIMER_RIGHT_41=1277,20
PRIMER_LEFT_41_TM=59.679
PRIMER_RIGHT_41_TM=58.840
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=547
PRIMER_PAIR_42_PENALTY=1.487963
PRIMER_LEFT_42_PENALTY=0.170049
PRIMER_RIGHT_42_PENALTY=1.317914
PRIMER_LEFT_42_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_42_SEQUENCE=AACCGACTTCAACCTCCACC
PRIMER_LEFT_42=682,20
PRIMER_RIGHT_42=1272,20
PRIMER_LEFT_42_TM=59.830
PRIMER_RIGHT_42_TM=61.318
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=591
PRIMER_PAIR_43_PENALTY=1.497662
PRIMER_LEFT_43_PENALTY=0.170049
PRIMER_RIGHT_43_PENALTY=1.327613
PRIMER_LEFT_43_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_43_SEQUENCE=ATAAGCGACGGATTCCACTT
PRIMER_LEFT_43=682,20
PRIMER_RIGHT_43=1122,20
PRIMER_LEFT_43_TM=59.830
PRIMER_RIGHT_43_TM=58.672
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=441
PRIMER_PAIR_44_PENALTY=1.497662
PRIMER_LEFT_44_PENALTY=0.170049
PRIMER_RIGHT_44_PENALTY=1.327613
PRIMER_LEFT_44_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_44_SEQUENCE=AATAAGCGACGGATTCCACT
PRIMER_LEFT_44=682,20
PRIMER_RIGHT_44=1123,20
PRIMER_LEFT_44_TM=59.830
PRIMER_RIGHT_44_TM=58.672
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=442
PRIMER_PAIR_45_PENALTY=1.509147
PRIMER_LEFT_45_PENALTY=0.445321
PRIMER_RIGHT_45_PENALTY=1.063825
PRIMER_LEFT_45_SEQUENCE=GGTGACTGGTTTTCTCGTCC
PRIMER_RIGHT_45_SEQUENCE=CAAACCGACTTCAACCTCCA
PRIMER_LEFT_45=769,20
PRIMER_RIGHT_45=1274,20
PRIMER_LEFT_45_TM=59.555
PRIMER_RIGHT_45_TM=61.064
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=506
PRIMER_PAIR_46_PENALTY=1.519383
PRIMER_LEFT_46_PENALTY=0.119028
PRIMER_RIGHT_46_PENALTY=1.400355
PRIMER_LEFT_46_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_46_SEQUENCE=GCAATGATTAATAAGCGACGG
PRIMER_LEFT_46=712,20
PRIMER_RIGHT_46=1132,21
PRIMER_LEFT_46_TM=59.881
PRIMER_RIGHT_46_TM=59.600
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=9.6000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=421
PRIMER_PAIR_47_PENALTY=1.555671
PRIMER_LEFT_47_PENALTY=0.491845
PRIMER_RIGHT_47_PENALTY=1.063825
PRIMER_LEFT_47_SEQUENCE=GGCTCTCATATGCCCAACAC
PRIMER_RIGHT_47_SEQUENCE=CAAACCGACTTCAACCTCCA
PRIMER_LEFT_47=730,20
PRIMER_RIGHT_47=1274,20
PRIMER_LEFT_47_TM=60.492
PRIMER_RIGHT_47_TM=61.064
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=2.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=545
PRIMER_PAIR_48_PENALTY=1.570404
PRIMER_LEFT_48_PENALTY=0.170049
PRIMER_RIGHT_48_PENALTY=1.400355
PRIMER_LEFT_48_SEQUENCE=CTCCGATGGTTGTTTTTGGT
PRIMER_RIGHT_48_SEQUENCE=GCAATGATTAATAAGCGACGG
PRIMER_LEFT_48=682,20
PRIMER_RIGHT_48=1132,21
PRIMER_LEFT_48_TM=59.830
PRIMER_RIGHT_48_TM=59.600
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=451
PRIMER_PAIR_49_PENALTY=1.584431
PRIMER_LEFT_49_PENALTY=0.119028
PRIMER_RIGHT_49_PENALTY=1.465403
PRIMER_LEFT_49_SEQUENCE=TGTTTCTTCACTTGCGTTGG
PRIMER_RIGHT_49_SEQUENCE=ATAAGCGACGGATTCCACTTC
PRIMER_LEFT_49=712,20
PRIMER_RIGHT_49=1122,21
PRIMER_LEFT_49_TM=59.881
PRIMER_RIGHT_49_TM=60.465
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=411
=
SEQUENCE_ID=MAGIC.105_2_8513683_8513691_bur-0_wil-2_116_48286_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=ATGTTATGGTNAAANGATATGATGCATAGCTGTGCAAAGANTAAAATTTAACTTCATATAAGGCTNCTTTTTGTGGCATATTGATGAAATATTAATNTTACACTTGGCTATTTTCATTGATCTGCTTAATGTTNCTGTTTATTGGTTTCATACGTGAATACAATCTAAACCAAATGATTGTGTAAATGTTTAGGTGGTGGACAACTACTACAGGCCGGACTTGAAGAAAGCTGCACTTGCTAGACTCAGCGCCATCAGCAAAGGTCTCAGGGTTGCCAAGTCNGGTGCCAAGCAAAGAAACAGACAAGCTTAAGCTTCTTCTTTCACAATCTGNTTTTTTTGAAGTAAAAGATTTTGTTGAACAGGTTTTATTTAAGTTGTTGCTTTGAAGTTGAACCATAANGCTATTCTCCTTTTTCNTTATCCTTATTATAAAGGAGTTAGAAGTTTATCTTNTTANNNNNNNNNNNNNNNNNNTGCTTGTGTTACATTTTGTTANATCNTTTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAACNAAGTCCTTATATNTNATGANNTTTGNAAGCCTANANNNNNNNNNNNNNNNNNNNNNNNNNNTTTANNTTNNTTGCATCANATGTGATGTTCTTGTGGACTNTAATGTTGACNTTNTCNTTNGATCCCATCAAAAACNAACTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACNGAAAGGTTTATATTAGGGTTTCNTAGACNTGNNTTAATTNTGTNCTCTNGCTGTNGCCGTCTCCAACNCCATTTCGAAGNNNNGNGAGANCGANGCNNNANNGAGNAAGNAAANNGNAGAAAGGAANNGGNNGNNANNANGGAGNNCNNNANCANNNNNNACANNNNNCTCCANAGNNNGTANNNNNTAAANCNTNAANNNTNNNNANCTTATNTNCANTNTCTCGTTTCCGNTTTNNTTAAGTTCGTTCTNGGNNTAGNNNNTAANNATTAGACTNAAATTGCAACTTNTNTTGATTNNAATTAGGTTTCTTGATGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANTCTNTAGATGAGTAANGATCATAAANATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTNGNNNCCTTTTATTGNATAGAAAGGTTGGCTTGTTGCTTAGGTNCATTTTNNATTNGAGNTTTTTTCGTTGTCANNATTGATCNNGTGGTNNNNNNNNNNNNNNNNNNNNTNNTTTCTAAATTGAACAGCACCTTCAAGAAGAAGGCACCCAATGCCATTAAAGAGATCAGGAAGTTTGCATTGAAAGCAATGGGAACNAANGACGTTAGAGTNGATGTTAAACTCAACAAGCAGATATGGAGCAAAGGTATCCGAGGTCCCCCGNGGAGNATNAGAGTCCGTGTTGCCCGTAAGAGAAACGATGATGAAGATGCCAAGGAAGAGTTCTTCTCNCTCGTCACNGTCGCTGAGATTCCGGCTGAAGGATTATCTGGTTTGGGTACTAAGGTCATTGAAGAAGAGGAGTGAAAATTGGTGAATGTACACTCACACANCCTTATCTTTNGATGNAAAGACGAAANNAAAAGACTTTGCTTGTTTTGATCACTTACCTATTTTGGTCATTTATCATNTCTTATGTTTTAAGTTCTATAGACAACAGATTTGGTACTACTCATACGAGTTTGATCTTTTTGTGACGCANTTTACTTCGCAATCATCTTTCTGATTCTGTACCAGACCGGTTTATTGTCTGGGTTGAAGTGTAACGAATTGGTTTGATACTCCACATTGTTAGGTTGGTGGAGTNTATCATAACTACTAAAAGGGTAGATAATCCNAAANTCCATATAAAATCAANGAGTATTACAAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_4_11038891_11039099_wil-2_edi-0_3120_2873214_TRUE
SEQUENCE_TARGET=950,308
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_19466843_19467573_hi-0_po-0_53436_384052_TRUE
SEQUENCE_TARGET=950,830
SEQUENCE_TEMPLATE=AATTCCAACTTCGCTCCACCTTTAAACTCAAAAATCAGCTCCGGCACCGTCACATCTCCCTTCCCGGAGATATTAAAACACGGTCCAAGTCCTGTTTCCTTCTCCAAGTCTTTCTCACGAGTGTAGTTCGACATCTGTGAAGCGAATTCTTCAGCAACGANCTCGAAAACGGGTCNCTCCATGAAAGTGAATGTAGATCCGGANTCAACAATGGATCCTCCGTNTCCGTTAGTTCCCGGCGCGAGATACTTATACGGAATCTTCACGTGCTTNNTTCCGACGTAGATTCGCCGGAGATTGAGGTAATAATACTCGAGAAAAGCTTTGTTGGAGACATTGGGATTTTTCCGAAACGGCGTGTANNTGAGACCCGGAGTTTTNGANCCGGAATTATGACCCGANCCGGTATCTAAATCAAGATCGGTGGTTACGTTGGTATCGTCAAACCGACGAGAAACCAAACAGTGAGAGAATCNCTTGAGATTCATTTGTGAAGGAAGCGATNCGGGTCCTCGCCCAAACCCAGCGATACCAGCGGGTTGTCGGNTGGAGATAATTGAACATCCGACTACGAAATCGGGTACGGTTAGATCCGGGAAATCAAGCTTTTCGGTTATTAAAACNCCAGCCGTTGATCCTAATCCGTATTGAAGAATGTACGGTGGACAACCGACGGTGCAGTTTCGGGTATTCGGGTCACATCCTCTGCATTGAACATTGGGTCCGTATAGAAACTNACATTTNGGANTCTGACACCCGATAATCTTCGAAGAGGAAGAGTTCTTNGGGATAAATCTAGGGATTAGAGTCGGATCCAAACCGGAGAAATCGCAACCGGAGCAGAGATATCTTGAGGTACAAGGTAGCCAAACGAGACTGCTTCCGGTATCGAAAACGAATGGGATTGTTTGCGACGGTGTGCCGAAGCTGAGAGAGACTGAGTATCCGCCGTAGCTTTTAGCAGANAGAGGAGATTTCACGACGGTGGCGGATGCGGTGNNGGTGGAGGAGAGAGCATCTTCGTCGGGTTTNATTGAGGTGCCGTGTTTGAGTTTATGAGCTCTAGCGATTGAGGATTCAGCGAGACGACGGAGAGAGAGGTAAGGGTCTTTNGGTGATTGGTCGGAGTGGGAGAAGGGAGAGAGAGGTAGTTTGACGGCGGAGACGACGGAGANGAAGATAANNNAGAAGAAGNNNNNAGNAGAAGNCATTGTNNTGGTGTGTTTGGGATTGATGGCTTATGGGTTAAGTAAGTGAGAAGGAGAAGAAAGAAGACAAAGAAGTGGAAAGTGGAGAATCATGTTCGATCTCTGTGACTTTCTTCTTCCTTTTATNAAAATGTTTTAGAATAATAATTTAGTTAAATGTGTCTGTTTTTACTTGGAAACTTATTACTAGTTACNACTATAAGTAAATCTTATACGTGGAAATGTNATTTTTGTTTAGTCGACATGATACATCTNTGTCTACGTAGACATGATGATACAGAGCCANCCAATACATTCTTGTATNTATAGAAAATGCCANNNNNNNNNNNNNNNNNNNNNNATNNTATATGTGTTTAATGNNNNNNNNNATACAAATAGTCAANTACTAATTATAGGATTATTTACGCCNTTCAAGTTGAAAAAAGAGCATGCTTATGTCGTTGAAAANTTCCAACTAAACAAATAAAACTAAGANAATTTGTGNGNAATCACCNTAGACCTATCTTTTTCAATTAATTGGCANGNNNATTGATTGGCCATGTGATGATCGCTCAAGGNGCTAACCATATTGACAAATCATGTGATGCATATTTATTAATCCATATCTTCTCTAACCAGATTTACNAGTTGCTTTAGTTGAATACATTTTGTCAGTGTTTTTGAGGAATGGAGTTTTTTCCCGTCCAAGTTNCATACTTCAGATTATTCACCANTTTTAAGTAATCATGTNCTCTGAANNTACTTANGAGGGAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTTTNCATAGTNAGATCNAGAATATCATATGAGATTNATATNNNNNNNNNATGTTCNNNTTGACTTCTTGTTTACATGTAACTTTTTGCNTATAGATTGATANAAANTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCGTAACATGAATCTCGACGTTATAAATCNANTGACNAAATAAAAAGCACGTTGAATACAATAGGTGTGAGNNATCGACCACATGNATATTTGTTGACTCTTATAAACTATATGACCTTAAACTTTTAAAAGTNGCAAACAGGTCATAAAACAAATAATTTNAAGGCTTTCCTTTCAAAGAAGTGACTTATTAATGCNNACATATTGATGAACAATNAANAAAAGTCAGTAAGTATATTTAACTNNNAAGACTTGAGANTATGTTTTAACAATTATGAACTTGAGAAAGTATTTTTGAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.023486
PRIMER_LEFT_0_PENALTY=0.238079
PRIMER_RIGHT_0_PENALTY=2.785407
PRIMER_LEFT_0_SEQUENCE=ATCGAAAACGAATGGGATTG
PRIMER_RIGHT_0_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_0=887,20
PRIMER_RIGHT_0=1879,22
PRIMER_LEFT_0_TM=59.762
PRIMER_RIGHT_0_TM=59.215
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=993
PRIMER_PAIR_1_PENALTY=3.023486
PRIMER_LEFT_1_PENALTY=0.238079
PRIMER_RIGHT_1_PENALTY=2.785407
PRIMER_LEFT_1_SEQUENCE=GGTATCGAAAACGAATGGGA
PRIMER_RIGHT_1_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_1=884,20
PRIMER_RIGHT_1=1879,22
PRIMER_LEFT_1_TM=59.762
PRIMER_RIGHT_1_TM=59.215
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=996
PRIMER_PAIR_2_PENALTY=3.077832
PRIMER_LEFT_2_PENALTY=0.292425
PRIMER_RIGHT_2_PENALTY=2.785407
PRIMER_LEFT_2_SEQUENCE=GTGCCGAAGCTGAGAGAGAC
PRIMER_RIGHT_2_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_2=918,20
PRIMER_RIGHT_2=1879,22
PRIMER_LEFT_2_TM=60.292
PRIMER_RIGHT_2_TM=59.215
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=962
PRIMER_PAIR_3_PENALTY=3.161437
PRIMER_LEFT_3_PENALTY=0.238079
PRIMER_RIGHT_3_PENALTY=2.923358
PRIMER_LEFT_3_SEQUENCE=ATCGAAAACGAATGGGATTG
PRIMER_RIGHT_3_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_3=887,20
PRIMER_RIGHT_3=1878,22
PRIMER_LEFT_3_TM=59.762
PRIMER_RIGHT_3_TM=59.077
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=992
PRIMER_PAIR_4_PENALTY=3.161437
PRIMER_LEFT_4_PENALTY=0.238079
PRIMER_RIGHT_4_PENALTY=2.923358
PRIMER_LEFT_4_SEQUENCE=GGTATCGAAAACGAATGGGA
PRIMER_RIGHT_4_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_4=884,20
PRIMER_RIGHT_4=1878,22
PRIMER_LEFT_4_TM=59.762
PRIMER_RIGHT_4_TM=59.077
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=995
PRIMER_PAIR_5_PENALTY=3.215783
PRIMER_LEFT_5_PENALTY=0.292425
PRIMER_RIGHT_5_PENALTY=2.923358
PRIMER_LEFT_5_SEQUENCE=GTGCCGAAGCTGAGAGAGAC
PRIMER_RIGHT_5_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_5=918,20
PRIMER_RIGHT_5=1878,22
PRIMER_LEFT_5_TM=60.292
PRIMER_RIGHT_5_TM=59.077
PRIMER_LEFT_5_GC_PERCENT=60.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.1000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=961
PRIMER_PAIR_6_PENALTY=3.216334
PRIMER_LEFT_6_PENALTY=0.430926
PRIMER_RIGHT_6_PENALTY=2.785407
PRIMER_LEFT_6_SEQUENCE=GCCGAAGCTGAGAGAGACTG
PRIMER_RIGHT_6_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_6=920,20
PRIMER_RIGHT_6=1879,22
PRIMER_LEFT_6_TM=60.431
PRIMER_RIGHT_6_TM=59.215
PRIMER_LEFT_6_GC_PERCENT=60.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=960
PRIMER_PAIR_7_PENALTY=3.264086
PRIMER_LEFT_7_PENALTY=0.238079
PRIMER_RIGHT_7_PENALTY=3.026007
PRIMER_LEFT_7_SEQUENCE=ATCGAAAACGAATGGGATTG
PRIMER_RIGHT_7_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_7=887,20
PRIMER_RIGHT_7=1879,23
PRIMER_LEFT_7_TM=59.762
PRIMER_RIGHT_7_TM=60.026
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=993
PRIMER_PAIR_8_PENALTY=3.264086
PRIMER_LEFT_8_PENALTY=0.238079
PRIMER_RIGHT_8_PENALTY=3.026007
PRIMER_LEFT_8_SEQUENCE=GGTATCGAAAACGAATGGGA
PRIMER_RIGHT_8_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_8=884,20
PRIMER_RIGHT_8=1879,23
PRIMER_LEFT_8_TM=59.762
PRIMER_RIGHT_8_TM=60.026
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=996
PRIMER_PAIR_9_PENALTY=3.318432
PRIMER_LEFT_9_PENALTY=0.292425
PRIMER_RIGHT_9_PENALTY=3.026007
PRIMER_LEFT_9_SEQUENCE=GTGCCGAAGCTGAGAGAGAC
PRIMER_RIGHT_9_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_9=918,20
PRIMER_RIGHT_9=1879,23
PRIMER_LEFT_9_TM=60.292
PRIMER_RIGHT_9_TM=60.026
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.1000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=962
PRIMER_PAIR_10_PENALTY=3.354285
PRIMER_LEFT_10_PENALTY=0.430926
PRIMER_RIGHT_10_PENALTY=2.923358
PRIMER_LEFT_10_SEQUENCE=GCCGAAGCTGAGAGAGACTG
PRIMER_RIGHT_10_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_10=920,20
PRIMER_RIGHT_10=1878,22
PRIMER_LEFT_10_TM=60.431
PRIMER_RIGHT_10_TM=59.077
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=959
PRIMER_PAIR_11_PENALTY=3.456934
PRIMER_LEFT_11_PENALTY=0.430926
PRIMER_RIGHT_11_PENALTY=3.026007
PRIMER_LEFT_11_SEQUENCE=GCCGAAGCTGAGAGAGACTG
PRIMER_RIGHT_11_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_11=920,20
PRIMER_RIGHT_11=1879,23
PRIMER_LEFT_11_TM=60.431
PRIMER_RIGHT_11_TM=60.026
PRIMER_LEFT_11_GC_PERCENT=60.000
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=960
PRIMER_PAIR_12_PENALTY=3.612006
PRIMER_LEFT_12_PENALTY=0.826599
PRIMER_RIGHT_12_PENALTY=2.785407
PRIMER_LEFT_12_SEQUENCE=TCCGGTATCGAAAACGAATG
PRIMER_RIGHT_12_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_12=881,20
PRIMER_RIGHT_12=1879,22
PRIMER_LEFT_12_TM=60.827
PRIMER_RIGHT_12_TM=59.215
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=999
PRIMER_PAIR_13_PENALTY=3.749957
PRIMER_LEFT_13_PENALTY=0.826599
PRIMER_RIGHT_13_PENALTY=2.923358
PRIMER_LEFT_13_SEQUENCE=TCCGGTATCGAAAACGAATG
PRIMER_RIGHT_13_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_13=881,20
PRIMER_RIGHT_13=1878,22
PRIMER_LEFT_13_TM=60.827
PRIMER_RIGHT_13_TM=59.077
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=998
PRIMER_PAIR_14_PENALTY=3.852606
PRIMER_LEFT_14_PENALTY=0.826599
PRIMER_RIGHT_14_PENALTY=3.026007
PRIMER_LEFT_14_SEQUENCE=TCCGGTATCGAAAACGAATG
PRIMER_RIGHT_14_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_14=881,20
PRIMER_RIGHT_14=1879,23
PRIMER_LEFT_14_TM=60.827
PRIMER_RIGHT_14_TM=60.026
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=999
PRIMER_PAIR_15_PENALTY=4.076931
PRIMER_LEFT_15_PENALTY=0.238079
PRIMER_RIGHT_15_PENALTY=3.838852
PRIMER_LEFT_15_SEQUENCE=ATCGAAAACGAATGGGATTG
PRIMER_RIGHT_15_SEQUENCE=CCATTCCTCAAAAACACTGA
PRIMER_LEFT_15=887,20
PRIMER_RIGHT_15=1877,20
PRIMER_LEFT_15_TM=59.762
PRIMER_RIGHT_15_TM=56.161
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=991
PRIMER_PAIR_16_PENALTY=4.076931
PRIMER_LEFT_16_PENALTY=0.238079
PRIMER_RIGHT_16_PENALTY=3.838852
PRIMER_LEFT_16_SEQUENCE=GGTATCGAAAACGAATGGGA
PRIMER_RIGHT_16_SEQUENCE=CCATTCCTCAAAAACACTGA
PRIMER_LEFT_16=884,20
PRIMER_RIGHT_16=1877,20
PRIMER_LEFT_16_TM=59.762
PRIMER_RIGHT_16_TM=56.161
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=994
PRIMER_PAIR_17_PENALTY=4.077795
PRIMER_LEFT_17_PENALTY=0.238079
PRIMER_RIGHT_17_PENALTY=3.839716
PRIMER_LEFT_17_SEQUENCE=ATCGAAAACGAATGGGATTG
PRIMER_RIGHT_17_SEQUENCE=CCATTCCTCAAAAACACTGAC
PRIMER_LEFT_17=887,20
PRIMER_RIGHT_17=1877,21
PRIMER_LEFT_17_TM=59.762
PRIMER_RIGHT_17_TM=57.160
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=991
PRIMER_PAIR_18_PENALTY=4.077795
PRIMER_LEFT_18_PENALTY=0.238079
PRIMER_RIGHT_18_PENALTY=3.839716
PRIMER_LEFT_18_SEQUENCE=GGTATCGAAAACGAATGGGA
PRIMER_RIGHT_18_SEQUENCE=CCATTCCTCAAAAACACTGAC
PRIMER_LEFT_18=884,20
PRIMER_RIGHT_18=1877,21
PRIMER_LEFT_18_TM=59.762
PRIMER_RIGHT_18_TM=57.160
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=994
PRIMER_PAIR_19_PENALTY=4.085063
PRIMER_LEFT_19_PENALTY=1.299656
PRIMER_RIGHT_19_PENALTY=2.785407
PRIMER_LEFT_19_SEQUENCE=GTGTGCCGAAGCTGAGAGAG
PRIMER_RIGHT_19_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_19=916,20
PRIMER_RIGHT_19=1879,22
PRIMER_LEFT_19_TM=61.300
PRIMER_RIGHT_19_TM=59.215
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=964
PRIMER_PAIR_20_PENALTY=4.131277
PRIMER_LEFT_20_PENALTY=0.292425
PRIMER_RIGHT_20_PENALTY=3.838852
PRIMER_LEFT_20_SEQUENCE=GTGCCGAAGCTGAGAGAGAC
PRIMER_RIGHT_20_SEQUENCE=CCATTCCTCAAAAACACTGA
PRIMER_LEFT_20=918,20
PRIMER_RIGHT_20=1877,20
PRIMER_LEFT_20_TM=60.292
PRIMER_RIGHT_20_TM=56.161
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.1000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=960
PRIMER_PAIR_21_PENALTY=4.132141
PRIMER_LEFT_21_PENALTY=0.292425
PRIMER_RIGHT_21_PENALTY=3.839716
PRIMER_LEFT_21_SEQUENCE=GTGCCGAAGCTGAGAGAGAC
PRIMER_RIGHT_21_SEQUENCE=CCATTCCTCAAAAACACTGAC
PRIMER_LEFT_21=918,20
PRIMER_RIGHT_21=1877,21
PRIMER_LEFT_21_TM=60.292
PRIMER_RIGHT_21_TM=57.160
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.1000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=960
PRIMER_PAIR_22_PENALTY=4.223014
PRIMER_LEFT_22_PENALTY=1.299656
PRIMER_RIGHT_22_PENALTY=2.923358
PRIMER_LEFT_22_SEQUENCE=GTGTGCCGAAGCTGAGAGAG
PRIMER_RIGHT_22_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_22=916,20
PRIMER_RIGHT_22=1878,22
PRIMER_LEFT_22_TM=61.300
PRIMER_RIGHT_22_TM=59.077
PRIMER_LEFT_22_GC_PERCENT=60.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=963
PRIMER_PAIR_23_PENALTY=4.269779
PRIMER_LEFT_23_PENALTY=0.430926
PRIMER_RIGHT_23_PENALTY=3.838852
PRIMER_LEFT_23_SEQUENCE=GCCGAAGCTGAGAGAGACTG
PRIMER_RIGHT_23_SEQUENCE=CCATTCCTCAAAAACACTGA
PRIMER_LEFT_23=920,20
PRIMER_RIGHT_23=1877,20
PRIMER_LEFT_23_TM=60.431
PRIMER_RIGHT_23_TM=56.161
PRIMER_LEFT_23_GC_PERCENT=60.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=958
PRIMER_PAIR_24_PENALTY=4.270643
PRIMER_LEFT_24_PENALTY=0.430926
PRIMER_RIGHT_24_PENALTY=3.839716
PRIMER_LEFT_24_SEQUENCE=GCCGAAGCTGAGAGAGACTG
PRIMER_RIGHT_24_SEQUENCE=CCATTCCTCAAAAACACTGAC
PRIMER_LEFT_24=920,20
PRIMER_RIGHT_24=1877,21
PRIMER_LEFT_24_TM=60.431
PRIMER_RIGHT_24_TM=57.160
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=958
PRIMER_PAIR_25_PENALTY=4.325663
PRIMER_LEFT_25_PENALTY=1.299656
PRIMER_RIGHT_25_PENALTY=3.026007
PRIMER_LEFT_25_SEQUENCE=GTGTGCCGAAGCTGAGAGAG
PRIMER_RIGHT_25_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_25=916,20
PRIMER_RIGHT_25=1879,23
PRIMER_LEFT_25_TM=61.300
PRIMER_RIGHT_25_TM=60.026
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=43.478
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=964
PRIMER_PAIR_26_PENALTY=4.378756
PRIMER_LEFT_26_PENALTY=1.593348
PRIMER_RIGHT_26_PENALTY=2.785407
PRIMER_LEFT_26_SEQUENCE=CCGAAGCTGAGAGAGACTGA
PRIMER_RIGHT_26_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_26=921,20
PRIMER_RIGHT_26=1879,22
PRIMER_LEFT_26_TM=58.407
PRIMER_RIGHT_26_TM=59.215
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=959
PRIMER_PAIR_27_PENALTY=4.516707
PRIMER_LEFT_27_PENALTY=1.593348
PRIMER_RIGHT_27_PENALTY=2.923358
PRIMER_LEFT_27_SEQUENCE=CCGAAGCTGAGAGAGACTGA
PRIMER_RIGHT_27_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_27=921,20
PRIMER_RIGHT_27=1878,22
PRIMER_LEFT_27_TM=58.407
PRIMER_RIGHT_27_TM=59.077
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=958
PRIMER_PAIR_28_PENALTY=4.619356
PRIMER_LEFT_28_PENALTY=1.593348
PRIMER_RIGHT_28_PENALTY=3.026007
PRIMER_LEFT_28_SEQUENCE=CCGAAGCTGAGAGAGACTGA
PRIMER_RIGHT_28_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_28=921,20
PRIMER_RIGHT_28=1879,23
PRIMER_LEFT_28_TM=58.407
PRIMER_RIGHT_28_TM=60.026
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=959
PRIMER_PAIR_29_PENALTY=4.665451
PRIMER_LEFT_29_PENALTY=0.826599
PRIMER_RIGHT_29_PENALTY=3.838852
PRIMER_LEFT_29_SEQUENCE=TCCGGTATCGAAAACGAATG
PRIMER_RIGHT_29_SEQUENCE=CCATTCCTCAAAAACACTGA
PRIMER_LEFT_29=881,20
PRIMER_RIGHT_29=1877,20
PRIMER_LEFT_29_TM=60.827
PRIMER_RIGHT_29_TM=56.161
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=997
PRIMER_PAIR_30_PENALTY=4.666315
PRIMER_LEFT_30_PENALTY=0.826599
PRIMER_RIGHT_30_PENALTY=3.839716
PRIMER_LEFT_30_SEQUENCE=TCCGGTATCGAAAACGAATG
PRIMER_RIGHT_30_SEQUENCE=CCATTCCTCAAAAACACTGAC
PRIMER_LEFT_30=881,20
PRIMER_RIGHT_30=1877,21
PRIMER_LEFT_30_TM=60.827
PRIMER_RIGHT_30_TM=57.160
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=997
PRIMER_PAIR_31_PENALTY=5.017164
PRIMER_LEFT_31_PENALTY=0.238079
PRIMER_RIGHT_31_PENALTY=4.779085
PRIMER_LEFT_31_SEQUENCE=ATCGAAAACGAATGGGATTG
PRIMER_RIGHT_31_SEQUENCE=ACTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_31=887,20
PRIMER_RIGHT_31=1880,24
PRIMER_LEFT_31_TM=59.762
PRIMER_RIGHT_31_TM=60.779
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=41.667
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=994
PRIMER_PAIR_32_PENALTY=5.017164
PRIMER_LEFT_32_PENALTY=0.238079
PRIMER_RIGHT_32_PENALTY=4.779085
PRIMER_LEFT_32_SEQUENCE=GGTATCGAAAACGAATGGGA
PRIMER_RIGHT_32_SEQUENCE=ACTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_32=884,20
PRIMER_RIGHT_32=1880,24
PRIMER_LEFT_32_TM=59.762
PRIMER_RIGHT_32_TM=60.779
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=41.667
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=997
PRIMER_PAIR_33_PENALTY=5.071510
PRIMER_LEFT_33_PENALTY=0.292425
PRIMER_RIGHT_33_PENALTY=4.779085
PRIMER_LEFT_33_SEQUENCE=GTGCCGAAGCTGAGAGAGAC
PRIMER_RIGHT_33_SEQUENCE=ACTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_33=918,20
PRIMER_RIGHT_33=1880,24
PRIMER_LEFT_33_TM=60.292
PRIMER_RIGHT_33_TM=60.779
PRIMER_LEFT_33_GC_PERCENT=60.000
PRIMER_RIGHT_33_GC_PERCENT=41.667
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.1000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=963
PRIMER_PAIR_34_PENALTY=5.138508
PRIMER_LEFT_34_PENALTY=1.299656
PRIMER_RIGHT_34_PENALTY=3.838852
PRIMER_LEFT_34_SEQUENCE=GTGTGCCGAAGCTGAGAGAG
PRIMER_RIGHT_34_SEQUENCE=CCATTCCTCAAAAACACTGA
PRIMER_LEFT_34=916,20
PRIMER_RIGHT_34=1877,20
PRIMER_LEFT_34_TM=61.300
PRIMER_RIGHT_34_TM=56.161
PRIMER_LEFT_34_GC_PERCENT=60.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=962
PRIMER_PAIR_35_PENALTY=5.139372
PRIMER_LEFT_35_PENALTY=1.299656
PRIMER_RIGHT_35_PENALTY=3.839716
PRIMER_LEFT_35_SEQUENCE=GTGTGCCGAAGCTGAGAGAG
PRIMER_RIGHT_35_SEQUENCE=CCATTCCTCAAAAACACTGAC
PRIMER_LEFT_35=916,20
PRIMER_RIGHT_35=1877,21
PRIMER_LEFT_35_TM=61.300
PRIMER_RIGHT_35_TM=57.160
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=962
PRIMER_PAIR_36_PENALTY=5.200062
PRIMER_LEFT_36_PENALTY=2.414654
PRIMER_RIGHT_36_PENALTY=2.785407
PRIMER_LEFT_36_SEQUENCE=TGTGCCGAAGCTGAGAGAGA
PRIMER_RIGHT_36_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_36=917,20
PRIMER_RIGHT_36=1879,22
PRIMER_LEFT_36_TM=62.415
PRIMER_RIGHT_36_TM=59.215
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=963
PRIMER_PAIR_37_PENALTY=5.210012
PRIMER_LEFT_37_PENALTY=0.430926
PRIMER_RIGHT_37_PENALTY=4.779085
PRIMER_LEFT_37_SEQUENCE=GCCGAAGCTGAGAGAGACTG
PRIMER_RIGHT_37_SEQUENCE=ACTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_37=920,20
PRIMER_RIGHT_37=1880,24
PRIMER_LEFT_37_TM=60.431
PRIMER_RIGHT_37_TM=60.779
PRIMER_LEFT_37_GC_PERCENT=60.000
PRIMER_RIGHT_37_GC_PERCENT=41.667
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=961
PRIMER_PAIR_38_PENALTY=5.229029
PRIMER_LEFT_38_PENALTY=2.443621
PRIMER_RIGHT_38_PENALTY=2.785407
PRIMER_LEFT_38_SEQUENCE=CGGTATCGAAAACGAATGGG
PRIMER_RIGHT_38_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_38=883,20
PRIMER_RIGHT_38=1879,22
PRIMER_LEFT_38_TM=62.444
PRIMER_RIGHT_38_TM=59.215
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=997
PRIMER_PAIR_39_PENALTY=5.229029
PRIMER_LEFT_39_PENALTY=2.443621
PRIMER_RIGHT_39_PENALTY=2.785407
PRIMER_LEFT_39_SEQUENCE=CCGGTATCGAAAACGAATGG
PRIMER_RIGHT_39_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_39=882,20
PRIMER_RIGHT_39=1879,22
PRIMER_LEFT_39_TM=62.444
PRIMER_RIGHT_39_TM=59.215
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.4000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=998
PRIMER_PAIR_40_PENALTY=5.332258
PRIMER_LEFT_40_PENALTY=2.546850
PRIMER_RIGHT_40_PENALTY=2.785407
PRIMER_LEFT_40_SEQUENCE=GTATCGAAAACGAATGGGATTG
PRIMER_RIGHT_40_SEQUENCE=CTCCATTCCTCAAAAACACTGA
PRIMER_LEFT_40=885,22
PRIMER_RIGHT_40=1879,22
PRIMER_LEFT_40_TM=60.547
PRIMER_RIGHT_40_TM=59.215
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=995
PRIMER_PAIR_41_PENALTY=5.338013
PRIMER_LEFT_41_PENALTY=2.414654
PRIMER_RIGHT_41_PENALTY=2.923358
PRIMER_LEFT_41_SEQUENCE=TGTGCCGAAGCTGAGAGAGA
PRIMER_RIGHT_41_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_41=917,20
PRIMER_RIGHT_41=1878,22
PRIMER_LEFT_41_TM=62.415
PRIMER_RIGHT_41_TM=59.077
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=962
PRIMER_PAIR_42_PENALTY=5.366980
PRIMER_LEFT_42_PENALTY=2.443621
PRIMER_RIGHT_42_PENALTY=2.923358
PRIMER_LEFT_42_SEQUENCE=CGGTATCGAAAACGAATGGG
PRIMER_RIGHT_42_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_42=883,20
PRIMER_RIGHT_42=1878,22
PRIMER_LEFT_42_TM=62.444
PRIMER_RIGHT_42_TM=59.077
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.6000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=996
PRIMER_PAIR_43_PENALTY=5.366980
PRIMER_LEFT_43_PENALTY=2.443621
PRIMER_RIGHT_43_PENALTY=2.923358
PRIMER_LEFT_43_SEQUENCE=CCGGTATCGAAAACGAATGG
PRIMER_RIGHT_43_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_43=882,20
PRIMER_RIGHT_43=1878,22
PRIMER_LEFT_43_TM=62.444
PRIMER_RIGHT_43_TM=59.077
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=997
PRIMER_PAIR_44_PENALTY=5.432201
PRIMER_LEFT_44_PENALTY=1.593348
PRIMER_RIGHT_44_PENALTY=3.838852
PRIMER_LEFT_44_SEQUENCE=CCGAAGCTGAGAGAGACTGA
PRIMER_RIGHT_44_SEQUENCE=CCATTCCTCAAAAACACTGA
PRIMER_LEFT_44=921,20
PRIMER_RIGHT_44=1877,20
PRIMER_LEFT_44_TM=58.407
PRIMER_RIGHT_44_TM=56.161
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=957
PRIMER_PAIR_45_PENALTY=5.433065
PRIMER_LEFT_45_PENALTY=1.593348
PRIMER_RIGHT_45_PENALTY=3.839716
PRIMER_LEFT_45_SEQUENCE=CCGAAGCTGAGAGAGACTGA
PRIMER_RIGHT_45_SEQUENCE=CCATTCCTCAAAAACACTGAC
PRIMER_LEFT_45=921,20
PRIMER_RIGHT_45=1877,21
PRIMER_LEFT_45_TM=58.407
PRIMER_RIGHT_45_TM=57.160
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=957
PRIMER_PAIR_46_PENALTY=5.440662
PRIMER_LEFT_46_PENALTY=2.414654
PRIMER_RIGHT_46_PENALTY=3.026007
PRIMER_LEFT_46_SEQUENCE=TGTGCCGAAGCTGAGAGAGA
PRIMER_RIGHT_46_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_46=917,20
PRIMER_RIGHT_46=1879,23
PRIMER_LEFT_46_TM=62.415
PRIMER_RIGHT_46_TM=60.026
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=43.478
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=963
PRIMER_PAIR_47_PENALTY=5.469629
PRIMER_LEFT_47_PENALTY=2.443621
PRIMER_RIGHT_47_PENALTY=3.026007
PRIMER_LEFT_47_SEQUENCE=CGGTATCGAAAACGAATGGG
PRIMER_RIGHT_47_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_47=883,20
PRIMER_RIGHT_47=1879,23
PRIMER_LEFT_47_TM=62.444
PRIMER_RIGHT_47_TM=60.026
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=997
PRIMER_PAIR_48_PENALTY=5.469629
PRIMER_LEFT_48_PENALTY=2.443621
PRIMER_RIGHT_48_PENALTY=3.026007
PRIMER_LEFT_48_SEQUENCE=CCGGTATCGAAAACGAATGG
PRIMER_RIGHT_48_SEQUENCE=CTCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_48=882,20
PRIMER_RIGHT_48=1879,23
PRIMER_LEFT_48_TM=62.444
PRIMER_RIGHT_48_TM=60.026
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=43.478
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=998
PRIMER_PAIR_49_PENALTY=5.470209
PRIMER_LEFT_49_PENALTY=2.546850
PRIMER_RIGHT_49_PENALTY=2.923358
PRIMER_LEFT_49_SEQUENCE=GTATCGAAAACGAATGGGATTG
PRIMER_RIGHT_49_SEQUENCE=TCCATTCCTCAAAAACACTGAC
PRIMER_LEFT_49=885,22
PRIMER_RIGHT_49=1878,22
PRIMER_LEFT_49_TM=60.547
PRIMER_RIGHT_49_TM=59.077
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=994
=
SEQUENCE_ID=MAGIC.433_2_5103465_5103475_kn-0_oy-0_112212_51188_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14637300_14637922_po-0_ws-0_68055_13657_TRUE
SEQUENCE_TARGET=950,722
SEQUENCE_TEMPLATE=GCTTCATGGTGCAAAGCATTCCATATTCNANTGTTNGTAGTTGAATTNATAAGAAGAACACNGAAACCAAGTGACTTTTTCTCATCATGNNANNNNNNNNTTGACAAAATAACAGAGATATTTGGATAAGTATCAACAGAAATAGANNCAAGAAGTAAAGGATTCAAGTCATCGANTCNTCTAGGCCTTTCCTTTGTNANAGCAATAAGATCTCCACATTTTGGCTGATACTTTTCTCTAATACTCAATGCATNANTTATTGACATATCGTAGAACAANTTGTTNGATNAGCTNCCACTTGATTCCTNNNNNNTTGTCTCCAAGGAACTNATTTCAANGAGANGAGCTTTTGACAAAGATTTGAAGCTTGNGGACAACTCGGTTCGCGTTTCCTCNAGTAGATGAGGAACNAAACACTTCAAGTATTCATTAACGGAACTAAACTTGTCTGGTATAGTCTTTATCTGTTACAAGAATAATAGACTATTGATTATAGCTATAAGTCTCNAAANATGTCAACTAANGAAGAAAGGTTAAAAGAAATGAAAACCTTTTGCTTGTAGAGATCTTTGTTGAGAATATCTTNCANAGACCAAGAAAATATTCTATCAAGNAGACTTGTTTTCTCGTTTCGTTCCATNATTCTTTCACTTTGACTAACTTTCTTTCCTGCAAGATTAAGACAAAGAAGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATACATAGATTAATAACACAAAATCAACCCTANAAATTGGAAACTTGGCTNNCCTTATGAACCTGNAACAAGCACAACAAGTNCAANAACAAAAANNANTAATGAAAAATTCATCTAAAATATTTGTCNNTCTNTCTATAATCCTTTTCTATGTCATNANTTCTTNTCATGGATACAATCCTTTTGCTAAAACCGTTGTCACAGTCACCAACAATATCAGCCCGCAAACAACCTTGACAATAAGTTGTAGATCTAAAGACGATGATCTNGGTGAGCATCTGTTGCTGCATNGGCAAGNGTTTCTATGNANGTTTAGACCAAGCTGGTTTAGAACAACGTTGTTCACNTGCAAGTTTCTTTGGAATAACAATGNCAAATGGTTTGATACATATAGATCTGACAGAGATCAAGGTCATTGTTATAGTTGTAATTGGTCTATCAANGCTGACAGTGCNTGTATTTCAGGAAACTTCAACAAAANNTTTGATAGGTGTTATCCATGGAATTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_14489856_14489949_ws-0_zu-0_14683_20951_TRUE
SEQUENCE_TARGET=950,193
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_16620673_16621088_ct-1_hi-0_27834_5355_TRUE
SEQUENCE_TARGET=950,515
SEQUENCE_TEMPLATE=AANNAANATNTATCNNAAATATATANNNTTAANTANNNTAATAANTAAGTTTNTNTNTTCNTAGAAATGTTTCTTCAGGCAATTTTACAACTCAGTGGCNAAAGTNGATTACCTCACACTTCGGCANGGGTTNATCTTTGTTAGTANCTCATTTTTTGTCAATGAATAATTANNTAAAAATANATAAATTTNAAATTTAATNTGTTNTTTTGANNATNGATNCAGGCTCATGTATCGTCAAATAATGCTTTCAACTTCCAAAACTATATACAGAGATCTTTACATGAAGATTTTAAAACTGTGGTTGGTATAAGGTAGTCANATTTGTGNATTTTACATGAATCTTAAGTAACAAAGTTAAATCAAATNAANTNNTTNANTTTNTTANAGTCCTTTAATGTGGCTNACTGTGGTCATCTTTATGCTCCTCGACGTTTNTGGTNACTTAATTTATATATTTNTTAGNTTAANTNNNTANNTTAAAATNNNTATTAGTGATTANTCGTTTTTATCTTATCTNNNNNNTTTNTTCTNAATCATCAGGTTGGAGAGTNTATTTTTATATGTCATTTGTGCCACTNATTGTAAGAGCTAATTNAATCTAGTNAANACTAANTNNNNNTATGTGTAACTCANATNATTNNNGAANTAATATTNTNNNNTAGATAGTTTTAGTGATCGGGACNAAACTAGAGATGATAGTAGCGAAAATGGCGGNCACAATTAAGGAGAATAACAGTGTAATTAGAGGAACCCCTCTCGTNGAGNCAAACGACACGCATTTCTGGTTTTCCAATCCTCGTTTTCTCTTAAGCATTCTACACTANACGCTGTTTCTGGTANTNTTTTNTAAACTACATGTNGNTTTTCCTANTGNTTCATCATANTNCNNNNNNNNNNNNNNNNNANACAGAACACNTTCGAGATGGCATTTATTGTGTGGATCACTGTAAGTTNNNANTTNNTNTAGGNATTTTAATATNTGGATTNATAAGAANNNNAAAAATAATATNGGAAANTGNTATANNNNNNNNNNNNNNNNNTTTAATTATGAAATTAGTGGCAATTTGGGATTAACTCTTGCTACCATGATAACCNAGGGATCATAATCACACGACTTGTANTAGCGTAAGCACTNCAATTTATTTATTTTATNCCACAAATTANTTACATTTTCNAAATTATATTTTNGTANTAAAAATCATATCGTTAATATTAAGNATANNNNNNNTTATAGGGTGACAGTCCAGTTCTTGAGCAGCTACATTACANTGCCTCTNTATGCCATTGTAACTCAGGTAATTNAGTCCNATATATNTAACACCANTTTTNAATTAAATCGTTNTNCATTAGTATANAAATTANTANTGTTAGTGTAANCTAGTGATCTGAATTTGNTTTGGATGCNNNNNNATNTGTGTNTAGATGGGGTCAAGCTACAAGAGAGCAATCTTAGAAGAACAACTAGCAAATGTGTTAAGGCACTGGCAAGGNATGGTTANAGANAAGNNNAAGACTANCCAAACGCCAGANACCNACAACAACAGTAACAATAACAATGGTGATATTGATTCTGGNGAAAGTCCNGTTCAGACAGAAGTTGCTTCTGAGTTTAGATTTTNGGGTAGACAATCGCCGATTTTACAAGAGATACAGATACAAGAGAAAACTGAAAGGTGATCAGATTGATGGAAAATGAGAAAGAGATTGTTNGTATTATTGTTANGAANTNTTTAGAGTTNCCTTNACTAATTATNGACGAATCAAATGACAAAATANTAANTTTAACCAGAAGTGTNAGTGTTANATTGGCATGNTGGTATCNNGATAACNTGTCACNCNNAATNTGANTAATNATTGATATNNTTTCTAGAGNATTGCTTGAAGCTATTTTTCTTATTGTGAGGTTATTAAGGACACTGTGAATTATATATACNTGTACAGAATGANAATGTNNCATTGGCAANGCAGAAAACAGACTTATCTCTATTCTTCTTTGAAAAGCTCGCGACCGATGACAATCCTTCTGATCTCGCTTGTCCCTGCACCGATTTCATATAGCTTTGCATCTCTTAGAAGGCGTCCTGTTGCATATTCGTTTATGTATCCATTTCCGCCTAAACATTGTATNGCCTGCAANATCAAACCAAATNTTTTAGTCTNAANGGNAAGGTAANNANNTNNNCAATATGTGTAGTTATGANCCNTTCNGAGGTNTGNTNTGCTGGTNACCATTAGAAAGACAAGAGNCAACGCTTAAAACNGNTTTNGGGNTTGATNTAGCTTTATGAGCTAGTGNCANAGTAACTNTAAGTANGAAGGAAANTAAGGGTANTTGACCTGTAAAGNGACCTGCGTTGCTCTCTCGGCNGCACAAAGAATAGTTCCGGCNCAGTCCTAGANAATAAGCAAATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=5.618626
PRIMER_LEFT_0_PENALTY=0.022341
PRIMER_RIGHT_0_PENALTY=5.596285
PRIMER_LEFT_0_SEQUENCE=ACACGCATTTCTGGTTTTCC
PRIMER_RIGHT_0_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_0=774,20
PRIMER_RIGHT_0=1691,24
PRIMER_LEFT_0_TM=59.978
PRIMER_RIGHT_0_TM=61.596
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=918
PRIMER_PAIR_1_PENALTY=5.673374
PRIMER_LEFT_1_PENALTY=0.022341
PRIMER_RIGHT_1_PENALTY=5.651033
PRIMER_LEFT_1_SEQUENCE=ACACGCATTTCTGGTTTTCC
PRIMER_RIGHT_1_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_1=774,20
PRIMER_RIGHT_1=1683,25
PRIMER_LEFT_1_TM=59.978
PRIMER_RIGHT_1_TM=60.651
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=910
PRIMER_PAIR_2_PENALTY=5.892111
PRIMER_LEFT_2_PENALTY=0.295825
PRIMER_RIGHT_2_PENALTY=5.596285
PRIMER_LEFT_2_SEQUENCE=GATAGTAGCGAAAATGGCGG
PRIMER_RIGHT_2_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_2=697,20
PRIMER_RIGHT_2=1691,24
PRIMER_LEFT_2_TM=59.704
PRIMER_RIGHT_2_TM=61.596
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=995
PRIMER_PAIR_3_PENALTY=5.946858
PRIMER_LEFT_3_PENALTY=0.295825
PRIMER_RIGHT_3_PENALTY=5.651033
PRIMER_LEFT_3_SEQUENCE=GATAGTAGCGAAAATGGCGG
PRIMER_RIGHT_3_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_3=697,20
PRIMER_RIGHT_3=1683,25
PRIMER_LEFT_3_TM=59.704
PRIMER_RIGHT_3_TM=60.651
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=987
PRIMER_PAIR_4_PENALTY=6.069980
PRIMER_LEFT_4_PENALTY=0.473695
PRIMER_RIGHT_4_PENALTY=5.596285
PRIMER_LEFT_4_SEQUENCE=TCTGGTTTTCCAATCCTCGT
PRIMER_RIGHT_4_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_4=783,20
PRIMER_RIGHT_4=1691,24
PRIMER_LEFT_4_TM=59.526
PRIMER_RIGHT_4_TM=61.596
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=909
PRIMER_PAIR_5_PENALTY=6.124727
PRIMER_LEFT_5_PENALTY=0.473695
PRIMER_RIGHT_5_PENALTY=5.651033
PRIMER_LEFT_5_SEQUENCE=TCTGGTTTTCCAATCCTCGT
PRIMER_RIGHT_5_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_5=783,20
PRIMER_RIGHT_5=1683,25
PRIMER_LEFT_5_TM=59.526
PRIMER_RIGHT_5_TM=60.651
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=901
PRIMER_PAIR_6_PENALTY=6.155660
PRIMER_LEFT_6_PENALTY=0.559375
PRIMER_RIGHT_6_PENALTY=5.596285
PRIMER_LEFT_6_SEQUENCE=ACGACACGCATTTCTGGTTT
PRIMER_RIGHT_6_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_6=771,20
PRIMER_RIGHT_6=1691,24
PRIMER_LEFT_6_TM=60.559
PRIMER_RIGHT_6_TM=61.596
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=41.667
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=921
PRIMER_PAIR_7_PENALTY=6.190535
PRIMER_LEFT_7_PENALTY=0.594250
PRIMER_RIGHT_7_PENALTY=5.596285
PRIMER_LEFT_7_SEQUENCE=TGGTTTTCCAATCCTCGTTT
PRIMER_RIGHT_7_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_7=785,20
PRIMER_RIGHT_7=1691,24
PRIMER_LEFT_7_TM=59.406
PRIMER_RIGHT_7_TM=61.596
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=41.667
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.7000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=907
PRIMER_PAIR_8_PENALTY=6.210407
PRIMER_LEFT_8_PENALTY=0.559375
PRIMER_RIGHT_8_PENALTY=5.651033
PRIMER_LEFT_8_SEQUENCE=ACGACACGCATTTCTGGTTT
PRIMER_RIGHT_8_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_8=771,20
PRIMER_RIGHT_8=1683,25
PRIMER_LEFT_8_TM=60.559
PRIMER_RIGHT_8_TM=60.651
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=913
PRIMER_PAIR_9_PENALTY=6.245283
PRIMER_LEFT_9_PENALTY=0.594250
PRIMER_RIGHT_9_PENALTY=5.651033
PRIMER_LEFT_9_SEQUENCE=TGGTTTTCCAATCCTCGTTT
PRIMER_RIGHT_9_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_9=785,20
PRIMER_RIGHT_9=1683,25
PRIMER_LEFT_9_TM=59.406
PRIMER_RIGHT_9_TM=60.651
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.7000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=899
PRIMER_PAIR_10_PENALTY=6.340128
PRIMER_LEFT_10_PENALTY=0.022341
PRIMER_RIGHT_10_PENALTY=6.317787
PRIMER_LEFT_10_SEQUENCE=ACACGCATTTCTGGTTTTCC
PRIMER_RIGHT_10_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_10=774,20
PRIMER_RIGHT_10=1683,22
PRIMER_LEFT_10_TM=59.978
PRIMER_RIGHT_10_TM=55.682
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=910
PRIMER_PAIR_11_PENALTY=6.568600
PRIMER_LEFT_11_PENALTY=0.972315
PRIMER_RIGHT_11_PENALTY=5.596285
PRIMER_LEFT_11_SEQUENCE=CTGGTTTTCCAATCCTCGTT
PRIMER_RIGHT_11_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_11=784,20
PRIMER_RIGHT_11=1691,24
PRIMER_LEFT_11_TM=59.028
PRIMER_RIGHT_11_TM=61.596
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=41.667
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=908
PRIMER_PAIR_12_PENALTY=6.613613
PRIMER_LEFT_12_PENALTY=0.295825
PRIMER_RIGHT_12_PENALTY=6.317787
PRIMER_LEFT_12_SEQUENCE=GATAGTAGCGAAAATGGCGG
PRIMER_RIGHT_12_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_12=697,20
PRIMER_RIGHT_12=1683,22
PRIMER_LEFT_12_TM=59.704
PRIMER_RIGHT_12_TM=55.682
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=12.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=987
PRIMER_PAIR_13_PENALTY=6.623347
PRIMER_LEFT_13_PENALTY=0.972315
PRIMER_RIGHT_13_PENALTY=5.651033
PRIMER_LEFT_13_SEQUENCE=CTGGTTTTCCAATCCTCGTT
PRIMER_RIGHT_13_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_13=784,20
PRIMER_RIGHT_13=1683,25
PRIMER_LEFT_13_TM=59.028
PRIMER_RIGHT_13_TM=60.651
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=900
PRIMER_PAIR_14_PENALTY=6.656499
PRIMER_LEFT_14_PENALTY=1.060213
PRIMER_RIGHT_14_PENALTY=5.596285
PRIMER_LEFT_14_SEQUENCE=CGACACGCATTTCTGGTTTT
PRIMER_RIGHT_14_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_14=772,20
PRIMER_RIGHT_14=1691,24
PRIMER_LEFT_14_TM=61.060
PRIMER_RIGHT_14_TM=61.596
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=41.667
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=920
PRIMER_PAIR_15_PENALTY=6.711246
PRIMER_LEFT_15_PENALTY=1.060213
PRIMER_RIGHT_15_PENALTY=5.651033
PRIMER_LEFT_15_SEQUENCE=CGACACGCATTTCTGGTTTT
PRIMER_RIGHT_15_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_15=772,20
PRIMER_RIGHT_15=1683,25
PRIMER_LEFT_15_TM=61.060
PRIMER_RIGHT_15_TM=60.651
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=912
PRIMER_PAIR_16_PENALTY=6.789817
PRIMER_LEFT_16_PENALTY=1.193532
PRIMER_RIGHT_16_PENALTY=5.596285
PRIMER_LEFT_16_SEQUENCE=GGTTTTCCAATCCTCGTTTTC
PRIMER_RIGHT_16_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_16=786,21
PRIMER_RIGHT_16=1691,24
PRIMER_LEFT_16_TM=59.806
PRIMER_RIGHT_16_TM=61.596
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=906
PRIMER_PAIR_17_PENALTY=6.791482
PRIMER_LEFT_17_PENALTY=0.473695
PRIMER_RIGHT_17_PENALTY=6.317787
PRIMER_LEFT_17_SEQUENCE=TCTGGTTTTCCAATCCTCGT
PRIMER_RIGHT_17_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_17=783,20
PRIMER_RIGHT_17=1683,22
PRIMER_LEFT_17_TM=59.526
PRIMER_RIGHT_17_TM=55.682
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=40.909
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=901
PRIMER_PAIR_18_PENALTY=6.844565
PRIMER_LEFT_18_PENALTY=1.193532
PRIMER_RIGHT_18_PENALTY=5.651033
PRIMER_LEFT_18_SEQUENCE=GGTTTTCCAATCCTCGTTTTC
PRIMER_RIGHT_18_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_18=786,21
PRIMER_RIGHT_18=1683,25
PRIMER_LEFT_18_TM=59.806
PRIMER_RIGHT_18_TM=60.651
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=898
PRIMER_PAIR_19_PENALTY=6.877162
PRIMER_LEFT_19_PENALTY=0.559375
PRIMER_RIGHT_19_PENALTY=6.317787
PRIMER_LEFT_19_SEQUENCE=ACGACACGCATTTCTGGTTT
PRIMER_RIGHT_19_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_19=771,20
PRIMER_RIGHT_19=1683,22
PRIMER_LEFT_19_TM=60.559
PRIMER_RIGHT_19_TM=55.682
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=913
PRIMER_PAIR_20_PENALTY=6.912037
PRIMER_LEFT_20_PENALTY=0.594250
PRIMER_RIGHT_20_PENALTY=6.317787
PRIMER_LEFT_20_SEQUENCE=TGGTTTTCCAATCCTCGTTT
PRIMER_RIGHT_20_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_20=785,20
PRIMER_RIGHT_20=1683,22
PRIMER_LEFT_20_TM=59.406
PRIMER_RIGHT_20_TM=55.682
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.7000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=899
PRIMER_PAIR_21_PENALTY=6.934636
PRIMER_LEFT_21_PENALTY=1.338351
PRIMER_RIGHT_21_PENALTY=5.596285
PRIMER_LEFT_21_SEQUENCE=CTGGTTTTCCAATCCTCGTTT
PRIMER_RIGHT_21_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_21=784,21
PRIMER_RIGHT_21=1691,24
PRIMER_LEFT_21_TM=60.338
PRIMER_RIGHT_21_TM=61.596
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=41.667
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.7000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=908
PRIMER_PAIR_22_PENALTY=6.989384
PRIMER_LEFT_22_PENALTY=1.338351
PRIMER_RIGHT_22_PENALTY=5.651033
PRIMER_LEFT_22_SEQUENCE=CTGGTTTTCCAATCCTCGTTT
PRIMER_RIGHT_22_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_22=784,21
PRIMER_RIGHT_22=1683,25
PRIMER_LEFT_22_TM=60.338
PRIMER_RIGHT_22_TM=60.651
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.7000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=900
PRIMER_PAIR_23_PENALTY=7.290102
PRIMER_LEFT_23_PENALTY=0.972315
PRIMER_RIGHT_23_PENALTY=6.317787
PRIMER_LEFT_23_SEQUENCE=CTGGTTTTCCAATCCTCGTT
PRIMER_RIGHT_23_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_23=784,20
PRIMER_RIGHT_23=1683,22
PRIMER_LEFT_23_TM=59.028
PRIMER_RIGHT_23_TM=55.682
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=900
PRIMER_PAIR_24_PENALTY=7.378001
PRIMER_LEFT_24_PENALTY=1.060213
PRIMER_RIGHT_24_PENALTY=6.317787
PRIMER_LEFT_24_SEQUENCE=CGACACGCATTTCTGGTTTT
PRIMER_RIGHT_24_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_24=772,20
PRIMER_RIGHT_24=1683,22
PRIMER_LEFT_24_TM=61.060
PRIMER_RIGHT_24_TM=55.682
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=912
PRIMER_PAIR_25_PENALTY=7.395412
PRIMER_LEFT_25_PENALTY=1.799127
PRIMER_RIGHT_25_PENALTY=5.596285
PRIMER_LEFT_25_SEQUENCE=GACACGCATTTCTGGTTTTC
PRIMER_RIGHT_25_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_25=773,20
PRIMER_RIGHT_25=1691,24
PRIMER_LEFT_25_TM=58.201
PRIMER_RIGHT_25_TM=61.596
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=41.667
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=919
PRIMER_PAIR_26_PENALTY=7.437206
PRIMER_LEFT_26_PENALTY=1.840921
PRIMER_RIGHT_26_PENALTY=5.596285
PRIMER_LEFT_26_SEQUENCE=TCTGGTTTTCCAATCCTCGTT
PRIMER_RIGHT_26_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_26=783,21
PRIMER_RIGHT_26=1691,24
PRIMER_LEFT_26_TM=60.841
PRIMER_RIGHT_26_TM=61.596
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=41.667
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=909
PRIMER_PAIR_27_PENALTY=7.437206
PRIMER_LEFT_27_PENALTY=1.840921
PRIMER_RIGHT_27_PENALTY=5.596285
PRIMER_LEFT_27_SEQUENCE=TTCTGGTTTTCCAATCCTCGT
PRIMER_RIGHT_27_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_27=782,21
PRIMER_RIGHT_27=1691,24
PRIMER_LEFT_27_TM=60.841
PRIMER_RIGHT_27_TM=61.596
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=41.667
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=910
PRIMER_PAIR_28_PENALTY=7.450159
PRIMER_LEFT_28_PENALTY=1.799127
PRIMER_RIGHT_28_PENALTY=5.651033
PRIMER_LEFT_28_SEQUENCE=GACACGCATTTCTGGTTTTC
PRIMER_RIGHT_28_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_28=773,20
PRIMER_RIGHT_28=1683,25
PRIMER_LEFT_28_TM=58.201
PRIMER_RIGHT_28_TM=60.651
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=911
PRIMER_PAIR_29_PENALTY=7.491954
PRIMER_LEFT_29_PENALTY=1.840921
PRIMER_RIGHT_29_PENALTY=5.651033
PRIMER_LEFT_29_SEQUENCE=TCTGGTTTTCCAATCCTCGTT
PRIMER_RIGHT_29_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_29=783,21
PRIMER_RIGHT_29=1683,25
PRIMER_LEFT_29_TM=60.841
PRIMER_RIGHT_29_TM=60.651
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=901
PRIMER_PAIR_30_PENALTY=7.491954
PRIMER_LEFT_30_PENALTY=1.840921
PRIMER_RIGHT_30_PENALTY=5.651033
PRIMER_LEFT_30_SEQUENCE=TTCTGGTTTTCCAATCCTCGT
PRIMER_RIGHT_30_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_30=782,21
PRIMER_RIGHT_30=1683,25
PRIMER_LEFT_30_TM=60.841
PRIMER_RIGHT_30_TM=60.651
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=902
PRIMER_PAIR_31_PENALTY=7.511319
PRIMER_LEFT_31_PENALTY=1.193532
PRIMER_RIGHT_31_PENALTY=6.317787
PRIMER_LEFT_31_SEQUENCE=GGTTTTCCAATCCTCGTTTTC
PRIMER_RIGHT_31_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_31=786,21
PRIMER_RIGHT_31=1683,22
PRIMER_LEFT_31_TM=59.806
PRIMER_RIGHT_31_TM=55.682
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.3000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=898
PRIMER_PAIR_32_PENALTY=7.544760
PRIMER_LEFT_32_PENALTY=1.948474
PRIMER_RIGHT_32_PENALTY=5.596285
PRIMER_LEFT_32_SEQUENCE=CGAGATGGCATTTATTGTGTG
PRIMER_RIGHT_32_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_32=921,21
PRIMER_RIGHT_32=1691,24
PRIMER_LEFT_32_TM=59.052
PRIMER_RIGHT_32_TM=61.596
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=41.667
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=771
PRIMER_PAIR_33_PENALTY=7.576773
PRIMER_LEFT_33_PENALTY=1.980488
PRIMER_RIGHT_33_PENALTY=5.596285
PRIMER_LEFT_33_SEQUENCE=GGTTTTCCAATCCTCGTTTT
PRIMER_RIGHT_33_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_33=786,20
PRIMER_RIGHT_33=1691,24
PRIMER_LEFT_33_TM=58.020
PRIMER_RIGHT_33_TM=61.596
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=41.667
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=906
PRIMER_PAIR_34_PENALTY=7.599507
PRIMER_LEFT_34_PENALTY=1.948474
PRIMER_RIGHT_34_PENALTY=5.651033
PRIMER_LEFT_34_SEQUENCE=CGAGATGGCATTTATTGTGTG
PRIMER_RIGHT_34_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_34=921,21
PRIMER_RIGHT_34=1683,25
PRIMER_LEFT_34_TM=59.052
PRIMER_RIGHT_34_TM=60.651
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=763
PRIMER_PAIR_35_PENALTY=7.631520
PRIMER_LEFT_35_PENALTY=1.980488
PRIMER_RIGHT_35_PENALTY=5.651033
PRIMER_LEFT_35_SEQUENCE=GGTTTTCCAATCCTCGTTTT
PRIMER_RIGHT_35_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_35=786,20
PRIMER_RIGHT_35=1683,25
PRIMER_LEFT_35_TM=58.020
PRIMER_RIGHT_35_TM=60.651
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=898
PRIMER_PAIR_36_PENALTY=7.656139
PRIMER_LEFT_36_PENALTY=1.338351
PRIMER_RIGHT_36_PENALTY=6.317787
PRIMER_LEFT_36_SEQUENCE=CTGGTTTTCCAATCCTCGTTT
PRIMER_RIGHT_36_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_36=784,21
PRIMER_RIGHT_36=1683,22
PRIMER_LEFT_36_TM=60.338
PRIMER_RIGHT_36_TM=55.682
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.7000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=900
PRIMER_PAIR_37_PENALTY=7.767110
PRIMER_LEFT_37_PENALTY=2.170825
PRIMER_RIGHT_37_PENALTY=5.596285
PRIMER_LEFT_37_SEQUENCE=GAGATGGCATTTATTGTGTGGA
PRIMER_RIGHT_37_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_37=922,22
PRIMER_RIGHT_37=1691,24
PRIMER_LEFT_37_TM=59.829
PRIMER_RIGHT_37_TM=61.596
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=41.667
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=770
PRIMER_PAIR_38_PENALTY=7.782107
PRIMER_LEFT_38_PENALTY=2.185822
PRIMER_RIGHT_38_PENALTY=5.596285
PRIMER_LEFT_38_SEQUENCE=CATTTCTGGTTTTCCAATCCTC
PRIMER_RIGHT_38_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_38=779,22
PRIMER_RIGHT_38=1691,24
PRIMER_LEFT_38_TM=59.814
PRIMER_RIGHT_38_TM=61.596
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=41.667
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=913
PRIMER_PAIR_39_PENALTY=7.821858
PRIMER_LEFT_39_PENALTY=2.170825
PRIMER_RIGHT_39_PENALTY=5.651033
PRIMER_LEFT_39_SEQUENCE=GAGATGGCATTTATTGTGTGGA
PRIMER_RIGHT_39_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_39=922,22
PRIMER_RIGHT_39=1683,25
PRIMER_LEFT_39_TM=59.829
PRIMER_RIGHT_39_TM=60.651
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=762
PRIMER_PAIR_40_PENALTY=7.836854
PRIMER_LEFT_40_PENALTY=2.185822
PRIMER_RIGHT_40_PENALTY=5.651033
PRIMER_LEFT_40_SEQUENCE=CATTTCTGGTTTTCCAATCCTC
PRIMER_RIGHT_40_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_40=779,22
PRIMER_RIGHT_40=1683,25
PRIMER_LEFT_40_TM=59.814
PRIMER_RIGHT_40_TM=60.651
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=905
PRIMER_PAIR_41_PENALTY=8.116914
PRIMER_LEFT_41_PENALTY=1.799127
PRIMER_RIGHT_41_PENALTY=6.317787
PRIMER_LEFT_41_SEQUENCE=GACACGCATTTCTGGTTTTC
PRIMER_RIGHT_41_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_41=773,20
PRIMER_RIGHT_41=1683,22
PRIMER_LEFT_41_TM=58.201
PRIMER_RIGHT_41_TM=55.682
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=911
PRIMER_PAIR_42_PENALTY=8.158709
PRIMER_LEFT_42_PENALTY=1.840921
PRIMER_RIGHT_42_PENALTY=6.317787
PRIMER_LEFT_42_SEQUENCE=TCTGGTTTTCCAATCCTCGTT
PRIMER_RIGHT_42_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_42=783,21
PRIMER_RIGHT_42=1683,22
PRIMER_LEFT_42_TM=60.841
PRIMER_RIGHT_42_TM=55.682
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=901
PRIMER_PAIR_43_PENALTY=8.158709
PRIMER_LEFT_43_PENALTY=1.840921
PRIMER_RIGHT_43_PENALTY=6.317787
PRIMER_LEFT_43_SEQUENCE=TTCTGGTTTTCCAATCCTCGT
PRIMER_RIGHT_43_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_43=782,21
PRIMER_RIGHT_43=1683,22
PRIMER_LEFT_43_TM=60.841
PRIMER_RIGHT_43_TM=55.682
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=902
PRIMER_PAIR_44_PENALTY=8.177276
PRIMER_LEFT_44_PENALTY=2.580991
PRIMER_RIGHT_44_PENALTY=5.596285
PRIMER_LEFT_44_SEQUENCE=CAAACGACACGCATTTCTGG
PRIMER_RIGHT_44_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_44=768,20
PRIMER_RIGHT_44=1691,24
PRIMER_LEFT_44_TM=62.581
PRIMER_RIGHT_44_TM=61.596
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=924
PRIMER_PAIR_45_PENALTY=8.232024
PRIMER_LEFT_45_PENALTY=2.580991
PRIMER_RIGHT_45_PENALTY=5.651033
PRIMER_LEFT_45_SEQUENCE=CAAACGACACGCATTTCTGG
PRIMER_RIGHT_45_SEQUENCE=CTGATCACCTTTCAGTTTTCTCTTG
PRIMER_LEFT_45=768,20
PRIMER_RIGHT_45=1683,25
PRIMER_LEFT_45_TM=62.581
PRIMER_RIGHT_45_TM=60.651
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=916
PRIMER_PAIR_46_PENALTY=8.241636
PRIMER_LEFT_46_PENALTY=0.022341
PRIMER_RIGHT_46_PENALTY=8.219295
PRIMER_LEFT_46_SEQUENCE=ACACGCATTTCTGGTTTTCC
PRIMER_RIGHT_46_SEQUENCE=TCCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_46=774,20
PRIMER_RIGHT_46=1692,25
PRIMER_LEFT_46_TM=59.978
PRIMER_RIGHT_46_TM=63.219
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=919
PRIMER_PAIR_47_PENALTY=8.266262
PRIMER_LEFT_47_PENALTY=1.948474
PRIMER_RIGHT_47_PENALTY=6.317787
PRIMER_LEFT_47_SEQUENCE=CGAGATGGCATTTATTGTGTG
PRIMER_RIGHT_47_SEQUENCE=CTGATCACCTTTCAGTTTTCTC
PRIMER_LEFT_47=921,21
PRIMER_RIGHT_47=1683,22
PRIMER_LEFT_47_TM=59.052
PRIMER_RIGHT_47_TM=55.682
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=763
PRIMER_PAIR_48_PENALTY=8.271466
PRIMER_LEFT_48_PENALTY=2.675181
PRIMER_RIGHT_48_PENALTY=5.596285
PRIMER_LEFT_48_SEQUENCE=GGTTTTCCAATCCTCGTTTTCT
PRIMER_RIGHT_48_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_48=786,22
PRIMER_RIGHT_48=1691,24
PRIMER_LEFT_48_TM=60.675
PRIMER_RIGHT_48_TM=61.596
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=41.667
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=906
PRIMER_PAIR_49_PENALTY=8.284698
PRIMER_LEFT_49_PENALTY=2.688413
PRIMER_RIGHT_49_PENALTY=5.596285
PRIMER_LEFT_49_SEQUENCE=CGCATTTCTGGTTTTCCAATC
PRIMER_RIGHT_49_SEQUENCE=CCATCAATCTGATCACCTTTCAGT
PRIMER_LEFT_49=777,21
PRIMER_RIGHT_49=1691,24
PRIMER_LEFT_49_TM=61.688
PRIMER_RIGHT_49_TM=61.596
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=41.667
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=915
=
SEQUENCE_ID=MAGIC.149_4_5341535_5341768_wil-2_edi-0_67019_15507_TRUE
SEQUENCE_TARGET=950,333
SEQUENCE_TEMPLATE=GGACTTGTAGTCAACCTTTGGAGTTGGACTGTAGTATGGTGGTGGTGGGGAGCTGTAGACGTATGGTGGTGGTGGAGACTTGTAGTCAACCTTTGGAGAAGGAGAGTAGTATGGTGGGGGTGGGGAACTGTAGACGTATGGGGGTGGTGGAGACTTGTAGTCACCCTTTGGAGATGGACTGTAGTATGGTGGTGGTGGAGAGCTGTAGACGTATGGTGGCGGTGGAGACTTGTAGTTAACCTTTGGTGAAGGAGAGTAGTATGGTGGGGGTGGGGAACTGTAGACGTATGGGGGTGGTAGGGACTTGTAGTCAACCTTTGGAGACGGAGAGTAGTATGGTGGTGGTGGGGAACTGTAAACGTATGATGGTGGTGGAGACTTGTAATCAACCTTTGGAGAAGGAGAGTAGTATGATGGAGGTGGTGAAGAGCTGTAAATAGATGGTTTTGGATGTGGTGTGTACTTAGGGATTTTGTGTTCATGGCTTGGAGAATTGTAATGTGGAGTTTGAGGGGATGTGTAAGGGTATGATGTCACTGTAGCCGCAATGGCACTTAGGACGACAACATAAACCATGCACTGTCCTAACCCCATCGTTCTTGAGGTTCTAATCACACCCATTGCAACTATCTTTTTTCTTTGTTTTGGTTTTATGTTATATTTGATCATGAACATCCTTTATATAGTTTTTNTTGGTGTAANTATAATATTATCTTCTCAACCTGGGTTCGTGTGTGGCACTTCCAAAAACATTAGAGGACGTGCTAAGTTGTNTGTTTGAAAAGTAGTGACTTGTGAGGAATATGAAGTATTATTAGATTCTCTAGCAACTCTCAAATTTNTTTAATACGTTTCCATATTCTTTTGTTTGAATTTGTCGACTCAAGTATTTTGAAGTACAAAACATATCCGTCAGTTGATGATTATTTTTAGCTATAAGTTTGTTAAATTTATTGTAACTTGNACGATTTACTTGGTTTTATAATNAAATANNNNNNANNAATTTAATTAATTTCATCGANATAAGAGTTCTATTGATTNAAAGTGAAACNAAAATAACCAATGTAGAAACAAAANACTCATGAAAACAAANGTTCAATAAAANTTTAAGGTGANCGATATACAAAGGTTCACANAAAGTTTCGATTTTTCCTCTCATAAGTCNTACCACACANTTTTTATATGCATNAAAAAATCTTAATGTANATTATNGGTNAAGTTTTTCTTGAAATTNGTACATAAATAATTTTGTAAACATCATGTATTTTCAANATATGANTATANTTAAGGTTTAAAANNTNTTTAAGCCGNCNAAAAATGATACAATTGATAAAANTATGGATGATATATCTTCTTTATATAAAAANATTTNAAACAANTCCAGATAANTATATGGNAGGGGAAGCATCAAAGCCAAACATTTACGGGANCAGCGTTGCAAAGCTGTAAAAAATGAAAGAAGAATTTGTTGAGGCCATTAAACATGTTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAAAATTTGTTTGGTTTCACGTTCATGCATTTCTATAACTCAATCNAANAAATCNTGTTNNNNTNAAATGTATATATNTGTAAAAGANTGTAAAATNNNNTATATNNCATAAAATAATTACTTTTATNCAAAATTGGATTATACATACGCTCGAATNAAAAGAAANAAAATTTGAATAGACTTCGGNTATATTATCTTCAATATGAAACCGATTGTCNGAGCNAATAAATTTAAATTGTTTAGACAAAAANATTTTAGTTNTNANAANANTGTTTTACTATATCTCAACTAGATNTGANATCACGGTTNTATATANTTGTTTTACATGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.102503
PRIMER_LEFT_0_PENALTY=0.074208
PRIMER_RIGHT_0_PENALTY=0.028296
PRIMER_LEFT_0_SEQUENCE=TAACCCCATCGTTCTTGAGG
PRIMER_RIGHT_0_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_0=585,20
PRIMER_RIGHT_0=1552,20
PRIMER_LEFT_0_TM=59.926
PRIMER_RIGHT_0_TM=59.972
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=968
PRIMER_PAIR_1_PENALTY=0.102503
PRIMER_LEFT_1_PENALTY=0.074208
PRIMER_RIGHT_1_PENALTY=0.028296
PRIMER_LEFT_1_SEQUENCE=TCCTAACCCCATCGTTCTTG
PRIMER_RIGHT_1_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_1=582,20
PRIMER_RIGHT_1=1552,20
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=59.972
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=971
PRIMER_PAIR_2_PENALTY=0.177690
PRIMER_LEFT_2_PENALTY=0.149395
PRIMER_RIGHT_2_PENALTY=0.028296
PRIMER_LEFT_2_SEQUENCE=ACCATGCACTGTCCTAACCC
PRIMER_RIGHT_2_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_2=571,20
PRIMER_RIGHT_2=1552,20
PRIMER_LEFT_2_TM=59.851
PRIMER_RIGHT_2_TM=59.972
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=982
PRIMER_PAIR_3_PENALTY=0.184356
PRIMER_LEFT_3_PENALTY=0.156061
PRIMER_RIGHT_3_PENALTY=0.028296
PRIMER_LEFT_3_SEQUENCE=CACTGTCCTAACCCCATCGT
PRIMER_RIGHT_3_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_3=577,20
PRIMER_RIGHT_3=1552,20
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=59.972
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=976
PRIMER_PAIR_4_PENALTY=0.422143
PRIMER_LEFT_4_PENALTY=0.074208
PRIMER_RIGHT_4_PENALTY=0.347935
PRIMER_LEFT_4_SEQUENCE=TAACCCCATCGTTCTTGAGG
PRIMER_RIGHT_4_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_4=585,20
PRIMER_RIGHT_4=1427,20
PRIMER_LEFT_4_TM=59.926
PRIMER_RIGHT_4_TM=60.348
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=843
PRIMER_PAIR_5_PENALTY=0.422143
PRIMER_LEFT_5_PENALTY=0.074208
PRIMER_RIGHT_5_PENALTY=0.347935
PRIMER_LEFT_5_SEQUENCE=TCCTAACCCCATCGTTCTTG
PRIMER_RIGHT_5_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_5=582,20
PRIMER_RIGHT_5=1427,20
PRIMER_LEFT_5_TM=59.926
PRIMER_RIGHT_5_TM=60.348
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=846
PRIMER_PAIR_6_PENALTY=0.437715
PRIMER_LEFT_6_PENALTY=0.409420
PRIMER_RIGHT_6_PENALTY=0.028296
PRIMER_LEFT_6_SEQUENCE=GTGTGGCACTTCCAAAAACA
PRIMER_RIGHT_6_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_6=732,20
PRIMER_RIGHT_6=1552,20
PRIMER_LEFT_6_TM=59.591
PRIMER_RIGHT_6_TM=59.972
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=821
PRIMER_PAIR_7_PENALTY=0.497330
PRIMER_LEFT_7_PENALTY=0.149395
PRIMER_RIGHT_7_PENALTY=0.347935
PRIMER_LEFT_7_SEQUENCE=ACCATGCACTGTCCTAACCC
PRIMER_RIGHT_7_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_7=571,20
PRIMER_RIGHT_7=1427,20
PRIMER_LEFT_7_TM=59.851
PRIMER_RIGHT_7_TM=60.348
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=857
PRIMER_PAIR_8_PENALTY=0.503996
PRIMER_LEFT_8_PENALTY=0.156061
PRIMER_RIGHT_8_PENALTY=0.347935
PRIMER_LEFT_8_SEQUENCE=CACTGTCCTAACCCCATCGT
PRIMER_RIGHT_8_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_8=577,20
PRIMER_RIGHT_8=1427,20
PRIMER_LEFT_8_TM=59.844
PRIMER_RIGHT_8_TM=60.348
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.0000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=851
PRIMER_PAIR_9_PENALTY=0.551302
PRIMER_LEFT_9_PENALTY=0.203367
PRIMER_RIGHT_9_PENALTY=0.347935
PRIMER_LEFT_9_SEQUENCE=GGGATTTTGTGTTCATGGCT
PRIMER_RIGHT_9_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_9=466,20
PRIMER_RIGHT_9=1427,20
PRIMER_LEFT_9_TM=59.797
PRIMER_RIGHT_9_TM=60.348
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=962
PRIMER_PAIR_10_PENALTY=0.563837
PRIMER_LEFT_10_PENALTY=0.215902
PRIMER_RIGHT_10_PENALTY=0.347935
PRIMER_LEFT_10_SEQUENCE=TTTGAGGGGATGTGTAAGGG
PRIMER_RIGHT_10_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_10=506,20
PRIMER_RIGHT_10=1427,20
PRIMER_LEFT_10_TM=59.784
PRIMER_RIGHT_10_TM=60.348
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=922
PRIMER_PAIR_11_PENALTY=0.568780
PRIMER_LEFT_11_PENALTY=0.220845
PRIMER_RIGHT_11_PENALTY=0.347935
PRIMER_LEFT_11_SEQUENCE=ATGTGGAGTTTGAGGGGATG
PRIMER_RIGHT_11_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_11=498,20
PRIMER_RIGHT_11=1427,20
PRIMER_LEFT_11_TM=59.779
PRIMER_RIGHT_11_TM=60.348
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=930
PRIMER_PAIR_12_PENALTY=0.587792
PRIMER_LEFT_12_PENALTY=0.239857
PRIMER_RIGHT_12_PENALTY=0.347935
PRIMER_LEFT_12_SEQUENCE=TGTGTTCATGGCTTGGAGAA
PRIMER_RIGHT_12_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_12=473,20
PRIMER_RIGHT_12=1427,20
PRIMER_LEFT_12_TM=60.240
PRIMER_RIGHT_12_TM=60.348
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=955
PRIMER_PAIR_13_PENALTY=0.622744
PRIMER_LEFT_13_PENALTY=0.074208
PRIMER_RIGHT_13_PENALTY=0.548537
PRIMER_LEFT_13_SEQUENCE=TAACCCCATCGTTCTTGAGG
PRIMER_RIGHT_13_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_13=585,20
PRIMER_RIGHT_13=1428,20
PRIMER_LEFT_13_TM=59.926
PRIMER_RIGHT_13_TM=59.451
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=844
PRIMER_PAIR_14_PENALTY=0.622744
PRIMER_LEFT_14_PENALTY=0.074208
PRIMER_RIGHT_14_PENALTY=0.548537
PRIMER_LEFT_14_SEQUENCE=TCCTAACCCCATCGTTCTTG
PRIMER_RIGHT_14_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_14=582,20
PRIMER_RIGHT_14=1428,20
PRIMER_LEFT_14_TM=59.926
PRIMER_RIGHT_14_TM=59.451
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=847
PRIMER_PAIR_15_PENALTY=0.623009
PRIMER_LEFT_15_PENALTY=0.594713
PRIMER_RIGHT_15_PENALTY=0.028296
PRIMER_LEFT_15_SEQUENCE=TGTCCTAACCCCATCGTTCT
PRIMER_RIGHT_15_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_15=580,20
PRIMER_RIGHT_15=1552,20
PRIMER_LEFT_15_TM=59.405
PRIMER_RIGHT_15_TM=59.972
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=973
PRIMER_PAIR_16_PENALTY=0.623009
PRIMER_LEFT_16_PENALTY=0.594713
PRIMER_RIGHT_16_PENALTY=0.028296
PRIMER_LEFT_16_SEQUENCE=CTGTCCTAACCCCATCGTTC
PRIMER_RIGHT_16_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_16=579,20
PRIMER_RIGHT_16=1552,20
PRIMER_LEFT_16_TM=59.405
PRIMER_RIGHT_16_TM=59.972
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=974
PRIMER_PAIR_17_PENALTY=0.632309
PRIMER_LEFT_17_PENALTY=0.284374
PRIMER_RIGHT_17_PENALTY=0.347935
PRIMER_LEFT_17_SEQUENCE=CAATGGCACTTAGGACGACA
PRIMER_RIGHT_17_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_17=545,20
PRIMER_RIGHT_17=1427,20
PRIMER_LEFT_17_TM=59.716
PRIMER_RIGHT_17_TM=60.348
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=883
PRIMER_PAIR_18_PENALTY=0.646877
PRIMER_LEFT_18_PENALTY=0.298942
PRIMER_RIGHT_18_PENALTY=0.347935
PRIMER_LEFT_18_SEQUENCE=TGGTTTTGGATGTGGTGTGT
PRIMER_RIGHT_18_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_18=441,20
PRIMER_RIGHT_18=1427,20
PRIMER_LEFT_18_TM=59.701
PRIMER_RIGHT_18_TM=60.348
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=987
PRIMER_PAIR_19_PENALTY=0.649082
PRIMER_LEFT_19_PENALTY=0.301147
PRIMER_RIGHT_19_PENALTY=0.347935
PRIMER_LEFT_19_SEQUENCE=TGGCACTTAGGACGACAACA
PRIMER_RIGHT_19_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_19=548,20
PRIMER_RIGHT_19=1427,20
PRIMER_LEFT_19_TM=60.301
PRIMER_RIGHT_19_TM=60.348
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=880
PRIMER_PAIR_20_PENALTY=0.656657
PRIMER_LEFT_20_PENALTY=0.308722
PRIMER_RIGHT_20_PENALTY=0.347935
PRIMER_LEFT_20_SEQUENCE=TTTGTGTTCATGGCTTGGAG
PRIMER_RIGHT_20_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_20=471,20
PRIMER_RIGHT_20=1427,20
PRIMER_LEFT_20_TM=59.691
PRIMER_RIGHT_20_TM=60.348
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=957
PRIMER_PAIR_21_PENALTY=0.697931
PRIMER_LEFT_21_PENALTY=0.149395
PRIMER_RIGHT_21_PENALTY=0.548537
PRIMER_LEFT_21_SEQUENCE=ACCATGCACTGTCCTAACCC
PRIMER_RIGHT_21_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_21=571,20
PRIMER_RIGHT_21=1428,20
PRIMER_LEFT_21_TM=59.851
PRIMER_RIGHT_21_TM=59.451
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=858
PRIMER_PAIR_22_PENALTY=0.704597
PRIMER_LEFT_22_PENALTY=0.156061
PRIMER_RIGHT_22_PENALTY=0.548537
PRIMER_LEFT_22_SEQUENCE=CACTGTCCTAACCCCATCGT
PRIMER_RIGHT_22_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_22=577,20
PRIMER_RIGHT_22=1428,20
PRIMER_LEFT_22_TM=59.844
PRIMER_RIGHT_22_TM=59.451
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.0000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=852
PRIMER_PAIR_23_PENALTY=0.710924
PRIMER_LEFT_23_PENALTY=0.362989
PRIMER_RIGHT_23_PENALTY=0.347935
PRIMER_LEFT_23_SEQUENCE=TGGAGTTTGAGGGGATGTGT
PRIMER_RIGHT_23_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_23=501,20
PRIMER_RIGHT_23=1427,20
PRIMER_LEFT_23_TM=60.363
PRIMER_RIGHT_23_TM=60.348
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=927
PRIMER_PAIR_24_PENALTY=0.710924
PRIMER_LEFT_24_PENALTY=0.362989
PRIMER_RIGHT_24_PENALTY=0.347935
PRIMER_LEFT_24_SEQUENCE=GTGGAGTTTGAGGGGATGTG
PRIMER_RIGHT_24_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_24=500,20
PRIMER_RIGHT_24=1427,20
PRIMER_LEFT_24_TM=60.363
PRIMER_RIGHT_24_TM=60.348
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=928
PRIMER_PAIR_25_PENALTY=0.710924
PRIMER_LEFT_25_PENALTY=0.362989
PRIMER_RIGHT_25_PENALTY=0.347935
PRIMER_LEFT_25_SEQUENCE=TGTGGAGTTTGAGGGGATGT
PRIMER_RIGHT_25_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_25=499,20
PRIMER_RIGHT_25=1427,20
PRIMER_LEFT_25_TM=60.363
PRIMER_RIGHT_25_TM=60.348
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.3000
PRIMER_RIGHT_25_END_STABILITY=7.3000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=929
PRIMER_PAIR_26_PENALTY=0.751903
PRIMER_LEFT_26_PENALTY=0.203367
PRIMER_RIGHT_26_PENALTY=0.548537
PRIMER_LEFT_26_SEQUENCE=GGGATTTTGTGTTCATGGCT
PRIMER_RIGHT_26_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_26=466,20
PRIMER_RIGHT_26=1428,20
PRIMER_LEFT_26_TM=59.797
PRIMER_RIGHT_26_TM=59.451
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=963
PRIMER_PAIR_27_PENALTY=0.764439
PRIMER_LEFT_27_PENALTY=0.215902
PRIMER_RIGHT_27_PENALTY=0.548537
PRIMER_LEFT_27_SEQUENCE=TTTGAGGGGATGTGTAAGGG
PRIMER_RIGHT_27_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_27=506,20
PRIMER_RIGHT_27=1428,20
PRIMER_LEFT_27_TM=59.784
PRIMER_RIGHT_27_TM=59.451
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=923
PRIMER_PAIR_28_PENALTY=0.769382
PRIMER_LEFT_28_PENALTY=0.220845
PRIMER_RIGHT_28_PENALTY=0.548537
PRIMER_LEFT_28_SEQUENCE=ATGTGGAGTTTGAGGGGATG
PRIMER_RIGHT_28_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_28=498,20
PRIMER_RIGHT_28=1428,20
PRIMER_LEFT_28_TM=59.779
PRIMER_RIGHT_28_TM=59.451
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.1000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=931
PRIMER_PAIR_29_PENALTY=0.773875
PRIMER_LEFT_29_PENALTY=0.745579
PRIMER_RIGHT_29_PENALTY=0.028296
PRIMER_LEFT_29_SEQUENCE=ACCCCATCGTTCTTGAGGTT
PRIMER_RIGHT_29_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_29=587,20
PRIMER_RIGHT_29=1552,20
PRIMER_LEFT_29_TM=60.746
PRIMER_RIGHT_29_TM=59.972
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=966
PRIMER_PAIR_30_PENALTY=0.788394
PRIMER_LEFT_30_PENALTY=0.239857
PRIMER_RIGHT_30_PENALTY=0.548537
PRIMER_LEFT_30_SEQUENCE=TGTGTTCATGGCTTGGAGAA
PRIMER_RIGHT_30_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_30=473,20
PRIMER_RIGHT_30=1428,20
PRIMER_LEFT_30_TM=60.240
PRIMER_RIGHT_30_TM=59.451
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=956
PRIMER_PAIR_31_PENALTY=0.832911
PRIMER_LEFT_31_PENALTY=0.284374
PRIMER_RIGHT_31_PENALTY=0.548537
PRIMER_LEFT_31_SEQUENCE=CAATGGCACTTAGGACGACA
PRIMER_RIGHT_31_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_31=545,20
PRIMER_RIGHT_31=1428,20
PRIMER_LEFT_31_TM=59.716
PRIMER_RIGHT_31_TM=59.451
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=884
PRIMER_PAIR_32_PENALTY=0.847478
PRIMER_LEFT_32_PENALTY=0.298942
PRIMER_RIGHT_32_PENALTY=0.548537
PRIMER_LEFT_32_SEQUENCE=TGGTTTTGGATGTGGTGTGT
PRIMER_RIGHT_32_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_32=441,20
PRIMER_RIGHT_32=1428,20
PRIMER_LEFT_32_TM=59.701
PRIMER_RIGHT_32_TM=59.451
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=988
PRIMER_PAIR_33_PENALTY=0.849684
PRIMER_LEFT_33_PENALTY=0.301147
PRIMER_RIGHT_33_PENALTY=0.548537
PRIMER_LEFT_33_SEQUENCE=TGGCACTTAGGACGACAACA
PRIMER_RIGHT_33_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_33=548,20
PRIMER_RIGHT_33=1428,20
PRIMER_LEFT_33_TM=60.301
PRIMER_RIGHT_33_TM=59.451
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=881
PRIMER_PAIR_34_PENALTY=0.857259
PRIMER_LEFT_34_PENALTY=0.308722
PRIMER_RIGHT_34_PENALTY=0.548537
PRIMER_LEFT_34_SEQUENCE=TTTGTGTTCATGGCTTGGAG
PRIMER_RIGHT_34_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_34=471,20
PRIMER_RIGHT_34=1428,20
PRIMER_LEFT_34_TM=59.691
PRIMER_RIGHT_34_TM=59.451
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=958
PRIMER_PAIR_35_PENALTY=0.891275
PRIMER_LEFT_35_PENALTY=0.862979
PRIMER_RIGHT_35_PENALTY=0.028296
PRIMER_LEFT_35_SEQUENCE=CCCATCGTTCTTGAGGTTCT
PRIMER_RIGHT_35_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_35=589,20
PRIMER_RIGHT_35=1552,20
PRIMER_LEFT_35_TM=59.137
PRIMER_RIGHT_35_TM=59.972
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=964
PRIMER_PAIR_36_PENALTY=0.911526
PRIMER_LEFT_36_PENALTY=0.362989
PRIMER_RIGHT_36_PENALTY=0.548537
PRIMER_LEFT_36_SEQUENCE=TGGAGTTTGAGGGGATGTGT
PRIMER_RIGHT_36_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_36=501,20
PRIMER_RIGHT_36=1428,20
PRIMER_LEFT_36_TM=60.363
PRIMER_RIGHT_36_TM=59.451
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=928
PRIMER_PAIR_37_PENALTY=0.911526
PRIMER_LEFT_37_PENALTY=0.362989
PRIMER_RIGHT_37_PENALTY=0.548537
PRIMER_LEFT_37_SEQUENCE=GTGGAGTTTGAGGGGATGTG
PRIMER_RIGHT_37_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_37=500,20
PRIMER_RIGHT_37=1428,20
PRIMER_LEFT_37_TM=60.363
PRIMER_RIGHT_37_TM=59.451
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=929
PRIMER_PAIR_38_PENALTY=0.911526
PRIMER_LEFT_38_PENALTY=0.362989
PRIMER_RIGHT_38_PENALTY=0.548537
PRIMER_LEFT_38_SEQUENCE=TGTGGAGTTTGAGGGGATGT
PRIMER_RIGHT_38_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_38=499,20
PRIMER_RIGHT_38=1428,20
PRIMER_LEFT_38_TM=60.363
PRIMER_RIGHT_38_TM=59.451
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.3000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=930
PRIMER_PAIR_39_PENALTY=0.942648
PRIMER_LEFT_39_PENALTY=0.594713
PRIMER_RIGHT_39_PENALTY=0.347935
PRIMER_LEFT_39_SEQUENCE=TGTCCTAACCCCATCGTTCT
PRIMER_RIGHT_39_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_39=580,20
PRIMER_RIGHT_39=1427,20
PRIMER_LEFT_39_TM=59.405
PRIMER_RIGHT_39_TM=60.348
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=848
PRIMER_PAIR_40_PENALTY=0.942648
PRIMER_LEFT_40_PENALTY=0.594713
PRIMER_RIGHT_40_PENALTY=0.347935
PRIMER_LEFT_40_SEQUENCE=CTGTCCTAACCCCATCGTTC
PRIMER_RIGHT_40_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_40=579,20
PRIMER_RIGHT_40=1427,20
PRIMER_LEFT_40_TM=59.405
PRIMER_RIGHT_40_TM=60.348
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.4000
PRIMER_RIGHT_40_END_STABILITY=7.3000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=849
PRIMER_PAIR_41_PENALTY=0.952080
PRIMER_LEFT_41_PENALTY=0.923785
PRIMER_RIGHT_41_PENALTY=0.028296
PRIMER_LEFT_41_SEQUENCE=TGCACTGTCCTAACCCCATC
PRIMER_RIGHT_41_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_41=575,20
PRIMER_RIGHT_41=1552,20
PRIMER_LEFT_41_TM=60.924
PRIMER_RIGHT_41_TM=59.972
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=978
PRIMER_PAIR_42_PENALTY=0.957956
PRIMER_LEFT_42_PENALTY=0.409420
PRIMER_RIGHT_42_PENALTY=0.548537
PRIMER_LEFT_42_SEQUENCE=GTGTGGCACTTCCAAAAACA
PRIMER_RIGHT_42_SEQUENCE=TCCCGTAAATGTTTGGCTTT
PRIMER_LEFT_42=732,20
PRIMER_RIGHT_42=1428,20
PRIMER_LEFT_42_TM=59.591
PRIMER_RIGHT_42_TM=59.451
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=697
PRIMER_PAIR_43_PENALTY=1.046462
PRIMER_LEFT_43_PENALTY=0.074208
PRIMER_RIGHT_43_PENALTY=0.972254
PRIMER_LEFT_43_SEQUENCE=TAACCCCATCGTTCTTGAGG
PRIMER_RIGHT_43_SEQUENCE=GCATGAACGTGAAACCAAAC
PRIMER_LEFT_43=585,20
PRIMER_RIGHT_43=1550,20
PRIMER_LEFT_43_TM=59.926
PRIMER_RIGHT_43_TM=59.028
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=1.046462
PRIMER_LEFT_44_PENALTY=0.074208
PRIMER_RIGHT_44_PENALTY=0.972254
PRIMER_LEFT_44_SEQUENCE=TCCTAACCCCATCGTTCTTG
PRIMER_RIGHT_44_SEQUENCE=GCATGAACGTGAAACCAAAC
PRIMER_LEFT_44=582,20
PRIMER_RIGHT_44=1550,20
PRIMER_LEFT_44_TM=59.926
PRIMER_RIGHT_44_TM=59.028
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=969
PRIMER_PAIR_45_PENALTY=1.046558
PRIMER_LEFT_45_PENALTY=1.018262
PRIMER_RIGHT_45_PENALTY=0.028296
PRIMER_LEFT_45_SEQUENCE=CTCAACCTGGGTTCGTGTGT
PRIMER_RIGHT_45_SEQUENCE=ATGCATGAACGTGAAACCAA
PRIMER_LEFT_45=716,20
PRIMER_RIGHT_45=1552,20
PRIMER_LEFT_45_TM=61.018
PRIMER_RIGHT_45_TM=59.972
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=837
PRIMER_PAIR_46_PENALTY=1.088750
PRIMER_LEFT_46_PENALTY=0.740815
PRIMER_RIGHT_46_PENALTY=0.347935
PRIMER_LEFT_46_SEQUENCE=TTGAGGGGATGTGTAAGGGT
PRIMER_RIGHT_46_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_46=507,20
PRIMER_RIGHT_46=1427,20
PRIMER_LEFT_46_TM=59.259
PRIMER_RIGHT_46_TM=60.348
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.1000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=921
PRIMER_PAIR_47_PENALTY=1.093514
PRIMER_LEFT_47_PENALTY=0.745579
PRIMER_RIGHT_47_PENALTY=0.347935
PRIMER_LEFT_47_SEQUENCE=ACCCCATCGTTCTTGAGGTT
PRIMER_RIGHT_47_SEQUENCE=CCCGTAAATGTTTGGCTTTG
PRIMER_LEFT_47=587,20
PRIMER_RIGHT_47=1427,20
PRIMER_LEFT_47_TM=60.746
PRIMER_RIGHT_47_TM=60.348
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=841
PRIMER_PAIR_48_PENALTY=1.121649
PRIMER_LEFT_48_PENALTY=0.149395
PRIMER_RIGHT_48_PENALTY=0.972254
PRIMER_LEFT_48_SEQUENCE=ACCATGCACTGTCCTAACCC
PRIMER_RIGHT_48_SEQUENCE=GCATGAACGTGAAACCAAAC
PRIMER_LEFT_48=571,20
PRIMER_RIGHT_48=1550,20
PRIMER_LEFT_48_TM=59.851
PRIMER_RIGHT_48_TM=59.028
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=980
PRIMER_PAIR_49_PENALTY=1.128315
PRIMER_LEFT_49_PENALTY=0.156061
PRIMER_RIGHT_49_PENALTY=0.972254
PRIMER_LEFT_49_SEQUENCE=CACTGTCCTAACCCCATCGT
PRIMER_RIGHT_49_SEQUENCE=GCATGAACGTGAAACCAAAC
PRIMER_LEFT_49=577,20
PRIMER_RIGHT_49=1550,20
PRIMER_LEFT_49_TM=59.844
PRIMER_RIGHT_49_TM=59.028
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.0000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=974
=
SEQUENCE_ID=MAGIC.175_3_10486874_10486994_kn-0_sf-2_5862_588413_TRUE
SEQUENCE_TARGET=950,220
SEQUENCE_TEMPLATE=NTAGATCATTCAAGTATTTGGTAAAAATGGCAGNCATTGANATTTTACAATGTAAAAGTATTTNCAANGCTTGANNTGTATCCCTAACCTTGCTCGCCTTCTTGACAGNTGGTCCCATGGCTGCTGCAACACCTCCAATGGTAGTCAATGTTTGCATCACAAGACTAAAAGTNGTCNNCGTGGACCACCGNATAATTCTACTACTGAACATGATANGAANGGTACATATAACAATTATAAGGATTCATGACTAATTATGTNATNAAAAANAAAACTTAAATAACAGAGAATTATAGAAAAATTCCTGAGTTCATACCAGTTCCATTTGGTTNGATACGTTTATTAGCNCCTTCCAGAATTTTNTTACAGCTTCAATTGGATCNNNACNTATCTACAAAAATTTNAAACACTCAGTTTCAACTCTACANCTTAGAATAANTTTTTCACAAAAGAATTGTTAGCAACAAAANAAGATTTCAAANNNNNNNNNNNNNNNNNNNNNNTACCNACGTCTANCTAANTCTCTTCGGNGCCGCAGTCCCCTGATNAACAACANACAAGTAAAAACAANAAATCTAAAAGACCATCCACAAAAATNAACCAAACAAACACATGTTAGGCAAAGCAGAAGTANNTTNAAAGGAATTCGTAAGTTTCAAAAGTGAGACACCAAAGTCATCAANTGCCGAAACAATCCANAATAAGCCTTCACTATGAACCTNGGGATTGTTGTNCTCTTNCATGATTNTGNAAAGCTACAANCNAAAGNCATGAATCATCANTGTGATCAGATNAAANTGAAAGTCGATAGAAAATTTANCGAACCANCACAAACCCTTTCGATCAGCTGCTTCACAAAAAGAAGTAGGCCACTTCATTGTAGAGGCTCATNTCTTTATATCTGAAACTGAGCCAAGAANNAATTNTGTAAGAAAAANATANAACTGACGTCTAAATACANCATTCAGTNNCTAAAAGTTNNNNNNNNNNNNNNTNNNANNNANNNNNNNNNNNNNNNATGACACATTTCTTTNGCCGCAGTTGAANNTATGAGNGATGATTTGNNNAANCTGCNTTTCCAAGTAATATTCTNAGGGAATAATTCGANAATTACATATAANAANATTTTANATGGANNAACAAGCATACNNCACANTTTTTCTCATTTTAACCTGGANCTGCACCTGCACACAACAACCGACCAAGATTTCCTCTNACTTGTCTAGTTCTNNNNNNNCTTCTATCTCTTCTTTCAAAGNCAAAGTTGATGCANGGCNNNNNAATATAATGCAAAATCATNAAACTCAGTAATANTTAACNNAAAAAAAGAGCTAAAAAAGAGCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGCATGTGCTAATGANGCTTTGATATGAATACAATGNANCATAGCATGGATATCAAATGCAGTAACAGAATAANAAANACTANAATTTAACAAGCANAAGAGATGCGTAAGGAAGATATCACCANCAATGTTGTCAAGATNCACAGNCCCACGACACAAANGAGCAGCNAAGGTACATTCTNTCACGTGCTTCTTGTTGTCACTTATNACCNAGAGNCAGAGAAATTANGAATGTGCAAGGGAAGNANNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.495457
PRIMER_LEFT_0_PENALTY=2.021762
PRIMER_RIGHT_0_PENALTY=0.473695
PRIMER_LEFT_0_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_0_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_0=609,21
PRIMER_RIGHT_0=1213,20
PRIMER_LEFT_0_TM=58.978
PRIMER_RIGHT_0_TM=59.526
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=605
PRIMER_PAIR_1_PENALTY=2.532548
PRIMER_LEFT_1_PENALTY=2.058853
PRIMER_RIGHT_1_PENALTY=0.473695
PRIMER_LEFT_1_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_1_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_1=609,22
PRIMER_RIGHT_1=1213,20
PRIMER_LEFT_1_TM=59.941
PRIMER_RIGHT_1_TM=59.526
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=605
PRIMER_PAIR_2_PENALTY=2.627585
PRIMER_LEFT_2_PENALTY=2.021762
PRIMER_RIGHT_2_PENALTY=0.605823
PRIMER_LEFT_2_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_2_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_2=609,21
PRIMER_RIGHT_2=1206,20
PRIMER_LEFT_2_TM=58.978
PRIMER_RIGHT_2_TM=60.606
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=598
PRIMER_PAIR_3_PENALTY=2.661021
PRIMER_LEFT_3_PENALTY=2.187326
PRIMER_RIGHT_3_PENALTY=0.473695
PRIMER_LEFT_3_SEQUENCE=ACACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_3_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_3=608,22
PRIMER_RIGHT_3=1213,20
PRIMER_LEFT_3_TM=59.813
PRIMER_RIGHT_3_TM=59.526
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=606
PRIMER_PAIR_4_PENALTY=2.661021
PRIMER_LEFT_4_PENALTY=2.187326
PRIMER_RIGHT_4_PENALTY=0.473695
PRIMER_LEFT_4_SEQUENCE=AACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_4_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_4=607,22
PRIMER_RIGHT_4=1213,20
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=607
PRIMER_PAIR_5_PENALTY=2.664676
PRIMER_LEFT_5_PENALTY=2.058853
PRIMER_RIGHT_5_PENALTY=0.605823
PRIMER_LEFT_5_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_5_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_5=609,22
PRIMER_RIGHT_5=1206,20
PRIMER_LEFT_5_TM=59.941
PRIMER_RIGHT_5_TM=60.606
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=598
PRIMER_PAIR_6_PENALTY=2.793149
PRIMER_LEFT_6_PENALTY=2.187326
PRIMER_RIGHT_6_PENALTY=0.605823
PRIMER_LEFT_6_SEQUENCE=ACACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_6_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_6=608,22
PRIMER_RIGHT_6=1206,20
PRIMER_LEFT_6_TM=59.813
PRIMER_RIGHT_6_TM=60.606
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=599
PRIMER_PAIR_7_PENALTY=2.793149
PRIMER_LEFT_7_PENALTY=2.187326
PRIMER_RIGHT_7_PENALTY=0.605823
PRIMER_LEFT_7_SEQUENCE=AACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_7_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_7=607,22
PRIMER_RIGHT_7=1206,20
PRIMER_LEFT_7_TM=59.813
PRIMER_RIGHT_7_TM=60.606
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=600
PRIMER_PAIR_8_PENALTY=2.881636
PRIMER_LEFT_8_PENALTY=2.407941
PRIMER_RIGHT_8_PENALTY=0.473695
PRIMER_LEFT_8_SEQUENCE=TCCTGAGTTCATACCAGTTCCA
PRIMER_RIGHT_8_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_8=302,22
PRIMER_RIGHT_8=1213,20
PRIMER_LEFT_8_TM=59.592
PRIMER_RIGHT_8_TM=59.526
PRIMER_LEFT_8_GC_PERCENT=45.455
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=912
PRIMER_PAIR_9_PENALTY=2.967619
PRIMER_LEFT_9_PENALTY=2.493925
PRIMER_RIGHT_9_PENALTY=0.473695
PRIMER_LEFT_9_SEQUENCE=CACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_9_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_9=609,20
PRIMER_RIGHT_9=1213,20
PRIMER_LEFT_9_TM=57.506
PRIMER_RIGHT_9_TM=59.526
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=605
PRIMER_PAIR_10_PENALTY=3.013764
PRIMER_LEFT_10_PENALTY=2.407941
PRIMER_RIGHT_10_PENALTY=0.605823
PRIMER_LEFT_10_SEQUENCE=TCCTGAGTTCATACCAGTTCCA
PRIMER_RIGHT_10_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_10=302,22
PRIMER_RIGHT_10=1206,20
PRIMER_LEFT_10_TM=59.592
PRIMER_RIGHT_10_TM=60.606
PRIMER_LEFT_10_GC_PERCENT=45.455
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=905
PRIMER_PAIR_11_PENALTY=3.027885
PRIMER_LEFT_11_PENALTY=2.554191
PRIMER_RIGHT_11_PENALTY=0.473695
PRIMER_LEFT_11_SEQUENCE=ACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_11_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_11=608,21
PRIMER_RIGHT_11=1213,20
PRIMER_LEFT_11_TM=58.446
PRIMER_RIGHT_11_TM=59.526
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=606
PRIMER_PAIR_12_PENALTY=3.099748
PRIMER_LEFT_12_PENALTY=2.493925
PRIMER_RIGHT_12_PENALTY=0.605823
PRIMER_LEFT_12_SEQUENCE=CACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_12_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_12=609,20
PRIMER_RIGHT_12=1206,20
PRIMER_LEFT_12_TM=57.506
PRIMER_RIGHT_12_TM=60.606
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=598
PRIMER_PAIR_13_PENALTY=3.135321
PRIMER_LEFT_13_PENALTY=2.021762
PRIMER_RIGHT_13_PENALTY=1.113558
PRIMER_LEFT_13_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_13_SEQUENCE=GGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_13=609,21
PRIMER_RIGHT_13=1211,20
PRIMER_LEFT_13_TM=58.978
PRIMER_RIGHT_13_TM=58.886
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=603
PRIMER_PAIR_14_PENALTY=3.138406
PRIMER_LEFT_14_PENALTY=2.021762
PRIMER_RIGHT_14_PENALTY=1.116644
PRIMER_LEFT_14_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_14_SEQUENCE=AAATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_14=609,21
PRIMER_RIGHT_14=1209,21
PRIMER_LEFT_14_TM=58.978
PRIMER_RIGHT_14_TM=59.883
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=601
PRIMER_PAIR_15_PENALTY=3.138726
PRIMER_LEFT_15_PENALTY=2.665032
PRIMER_RIGHT_15_PENALTY=0.473695
PRIMER_LEFT_15_SEQUENCE=AAACACATGTTAGGCAAAGCAG
PRIMER_RIGHT_15_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_15=606,22
PRIMER_RIGHT_15=1213,20
PRIMER_LEFT_15_TM=59.335
PRIMER_RIGHT_15_TM=59.526
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=608
PRIMER_PAIR_16_PENALTY=3.160014
PRIMER_LEFT_16_PENALTY=2.554191
PRIMER_RIGHT_16_PENALTY=0.605823
PRIMER_LEFT_16_SEQUENCE=ACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_16_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_16=608,21
PRIMER_RIGHT_16=1206,20
PRIMER_LEFT_16_TM=58.446
PRIMER_RIGHT_16_TM=60.606
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=599
PRIMER_PAIR_17_PENALTY=3.165513
PRIMER_LEFT_17_PENALTY=2.021762
PRIMER_RIGHT_17_PENALTY=1.143751
PRIMER_LEFT_17_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_17_SEQUENCE=AGGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_17=609,21
PRIMER_RIGHT_17=1212,21
PRIMER_LEFT_17_TM=58.978
PRIMER_RIGHT_17_TM=59.856
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=604
PRIMER_PAIR_18_PENALTY=3.172412
PRIMER_LEFT_18_PENALTY=2.058853
PRIMER_RIGHT_18_PENALTY=1.113558
PRIMER_LEFT_18_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_18_SEQUENCE=GGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_18=609,22
PRIMER_RIGHT_18=1211,20
PRIMER_LEFT_18_TM=59.941
PRIMER_RIGHT_18_TM=58.886
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=603
PRIMER_PAIR_19_PENALTY=3.175497
PRIMER_LEFT_19_PENALTY=2.058853
PRIMER_RIGHT_19_PENALTY=1.116644
PRIMER_LEFT_19_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_19_SEQUENCE=AAATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_19=609,22
PRIMER_RIGHT_19=1209,21
PRIMER_LEFT_19_TM=59.941
PRIMER_RIGHT_19_TM=59.883
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=601
PRIMER_PAIR_20_PENALTY=3.202604
PRIMER_LEFT_20_PENALTY=2.058853
PRIMER_RIGHT_20_PENALTY=1.143751
PRIMER_LEFT_20_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_20_SEQUENCE=AGGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_20=609,22
PRIMER_RIGHT_20=1212,21
PRIMER_LEFT_20_TM=59.941
PRIMER_RIGHT_20_TM=59.856
PRIMER_LEFT_20_GC_PERCENT=45.455
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=604
PRIMER_PAIR_21_PENALTY=3.270855
PRIMER_LEFT_21_PENALTY=2.665032
PRIMER_RIGHT_21_PENALTY=0.605823
PRIMER_LEFT_21_SEQUENCE=AAACACATGTTAGGCAAAGCAG
PRIMER_RIGHT_21_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_21=606,22
PRIMER_RIGHT_21=1206,20
PRIMER_LEFT_21_TM=59.335
PRIMER_RIGHT_21_TM=60.606
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=601
PRIMER_PAIR_22_PENALTY=3.285273
PRIMER_LEFT_22_PENALTY=2.811578
PRIMER_RIGHT_22_PENALTY=0.473695
PRIMER_LEFT_22_SEQUENCE=CCAAACAAACACATGTTAGGCA
PRIMER_RIGHT_22_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_22=600,22
PRIMER_RIGHT_22=1213,20
PRIMER_LEFT_22_TM=60.812
PRIMER_RIGHT_22_TM=59.526
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=614
PRIMER_PAIR_23_PENALTY=3.300884
PRIMER_LEFT_23_PENALTY=2.187326
PRIMER_RIGHT_23_PENALTY=1.113558
PRIMER_LEFT_23_SEQUENCE=ACACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_23_SEQUENCE=GGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_23=608,22
PRIMER_RIGHT_23=1211,20
PRIMER_LEFT_23_TM=59.813
PRIMER_RIGHT_23_TM=58.886
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=604
PRIMER_PAIR_24_PENALTY=3.300884
PRIMER_LEFT_24_PENALTY=2.187326
PRIMER_RIGHT_24_PENALTY=1.113558
PRIMER_LEFT_24_SEQUENCE=AACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_24_SEQUENCE=GGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_24=607,22
PRIMER_RIGHT_24=1211,20
PRIMER_LEFT_24_TM=59.813
PRIMER_RIGHT_24_TM=58.886
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=605
PRIMER_PAIR_25_PENALTY=3.303970
PRIMER_LEFT_25_PENALTY=2.187326
PRIMER_RIGHT_25_PENALTY=1.116644
PRIMER_LEFT_25_SEQUENCE=ACACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_25_SEQUENCE=AAATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_25=608,22
PRIMER_RIGHT_25=1209,21
PRIMER_LEFT_25_TM=59.813
PRIMER_RIGHT_25_TM=59.883
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=602
PRIMER_PAIR_26_PENALTY=3.303970
PRIMER_LEFT_26_PENALTY=2.187326
PRIMER_RIGHT_26_PENALTY=1.116644
PRIMER_LEFT_26_SEQUENCE=AACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_26_SEQUENCE=AAATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_26=607,22
PRIMER_RIGHT_26=1209,21
PRIMER_LEFT_26_TM=59.813
PRIMER_RIGHT_26_TM=59.883
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=603
PRIMER_PAIR_27_PENALTY=3.331077
PRIMER_LEFT_27_PENALTY=2.187326
PRIMER_RIGHT_27_PENALTY=1.143751
PRIMER_LEFT_27_SEQUENCE=ACACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_27_SEQUENCE=AGGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_27=608,22
PRIMER_RIGHT_27=1212,21
PRIMER_LEFT_27_TM=59.813
PRIMER_RIGHT_27_TM=59.856
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=605
PRIMER_PAIR_28_PENALTY=3.331077
PRIMER_LEFT_28_PENALTY=2.187326
PRIMER_RIGHT_28_PENALTY=1.143751
PRIMER_LEFT_28_SEQUENCE=AACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_28_SEQUENCE=AGGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_28=607,22
PRIMER_RIGHT_28=1212,21
PRIMER_LEFT_28_TM=59.813
PRIMER_RIGHT_28_TM=59.856
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=606
PRIMER_PAIR_29_PENALTY=3.376796
PRIMER_LEFT_29_PENALTY=2.021762
PRIMER_RIGHT_29_PENALTY=1.355034
PRIMER_LEFT_29_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_29_SEQUENCE=GAGGAAATCTTGGTCGGTTGT
PRIMER_LEFT_29=609,21
PRIMER_RIGHT_29=1213,21
PRIMER_LEFT_29_TM=58.978
PRIMER_RIGHT_29_TM=60.355
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=605
PRIMER_PAIR_30_PENALTY=3.413887
PRIMER_LEFT_30_PENALTY=2.058853
PRIMER_RIGHT_30_PENALTY=1.355034
PRIMER_LEFT_30_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_30_SEQUENCE=GAGGAAATCTTGGTCGGTTGT
PRIMER_LEFT_30=609,22
PRIMER_RIGHT_30=1213,21
PRIMER_LEFT_30_TM=59.941
PRIMER_RIGHT_30_TM=60.355
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=605
PRIMER_PAIR_31_PENALTY=3.417401
PRIMER_LEFT_31_PENALTY=2.811578
PRIMER_RIGHT_31_PENALTY=0.605823
PRIMER_LEFT_31_SEQUENCE=CCAAACAAACACATGTTAGGCA
PRIMER_RIGHT_31_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_31=600,22
PRIMER_RIGHT_31=1206,20
PRIMER_LEFT_31_TM=60.812
PRIMER_RIGHT_31_TM=60.606
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.7000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=607
PRIMER_PAIR_32_PENALTY=3.499067
PRIMER_LEFT_32_PENALTY=3.025372
PRIMER_RIGHT_32_PENALTY=0.473695
PRIMER_LEFT_32_SEQUENCE=AACACATGTTAGGCAAAGCAG
PRIMER_RIGHT_32_SEQUENCE=GAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_32=607,21
PRIMER_RIGHT_32=1213,20
PRIMER_LEFT_32_TM=57.975
PRIMER_RIGHT_32_TM=59.526
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=607
PRIMER_PAIR_33_PENALTY=3.503425
PRIMER_LEFT_33_PENALTY=2.021762
PRIMER_RIGHT_33_PENALTY=1.481663
PRIMER_LEFT_33_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_33_SEQUENCE=AGAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_33=609,21
PRIMER_RIGHT_33=1214,21
PRIMER_LEFT_33_TM=58.978
PRIMER_RIGHT_33_TM=60.482
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=606
PRIMER_PAIR_34_PENALTY=3.521500
PRIMER_LEFT_34_PENALTY=2.407941
PRIMER_RIGHT_34_PENALTY=1.113558
PRIMER_LEFT_34_SEQUENCE=TCCTGAGTTCATACCAGTTCCA
PRIMER_RIGHT_34_SEQUENCE=GGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_34=302,22
PRIMER_RIGHT_34=1211,20
PRIMER_LEFT_34_TM=59.592
PRIMER_RIGHT_34_TM=58.886
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=910
PRIMER_PAIR_35_PENALTY=3.523045
PRIMER_LEFT_35_PENALTY=2.021762
PRIMER_RIGHT_35_PENALTY=1.501283
PRIMER_LEFT_35_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_35_SEQUENCE=AGGAAATCTTGGTCGGTTGT
PRIMER_LEFT_35=609,21
PRIMER_RIGHT_35=1212,20
PRIMER_LEFT_35_TM=58.978
PRIMER_RIGHT_35_TM=58.499
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=604
PRIMER_PAIR_36_PENALTY=3.524585
PRIMER_LEFT_36_PENALTY=2.407941
PRIMER_RIGHT_36_PENALTY=1.116644
PRIMER_LEFT_36_SEQUENCE=TCCTGAGTTCATACCAGTTCCA
PRIMER_RIGHT_36_SEQUENCE=AAATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_36=302,22
PRIMER_RIGHT_36=1209,21
PRIMER_LEFT_36_TM=59.592
PRIMER_RIGHT_36_TM=59.883
PRIMER_LEFT_36_GC_PERCENT=45.455
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=908
PRIMER_PAIR_37_PENALTY=3.540516
PRIMER_LEFT_37_PENALTY=2.058853
PRIMER_RIGHT_37_PENALTY=1.481663
PRIMER_LEFT_37_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_37_SEQUENCE=AGAGGAAATCTTGGTCGGTTG
PRIMER_LEFT_37=609,22
PRIMER_RIGHT_37=1214,21
PRIMER_LEFT_37_TM=59.941
PRIMER_RIGHT_37_TM=60.482
PRIMER_LEFT_37_GC_PERCENT=45.455
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=606
PRIMER_PAIR_38_PENALTY=3.542360
PRIMER_LEFT_38_PENALTY=2.187326
PRIMER_RIGHT_38_PENALTY=1.355034
PRIMER_LEFT_38_SEQUENCE=ACACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_38_SEQUENCE=GAGGAAATCTTGGTCGGTTGT
PRIMER_LEFT_38=608,22
PRIMER_RIGHT_38=1213,21
PRIMER_LEFT_38_TM=59.813
PRIMER_RIGHT_38_TM=60.355
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=606
PRIMER_PAIR_39_PENALTY=3.542360
PRIMER_LEFT_39_PENALTY=2.187326
PRIMER_RIGHT_39_PENALTY=1.355034
PRIMER_LEFT_39_SEQUENCE=AACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_39_SEQUENCE=GAGGAAATCTTGGTCGGTTGT
PRIMER_LEFT_39=607,22
PRIMER_RIGHT_39=1213,21
PRIMER_LEFT_39_TM=59.813
PRIMER_RIGHT_39_TM=60.355
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=607
PRIMER_PAIR_40_PENALTY=3.551692
PRIMER_LEFT_40_PENALTY=2.407941
PRIMER_RIGHT_40_PENALTY=1.143751
PRIMER_LEFT_40_SEQUENCE=TCCTGAGTTCATACCAGTTCCA
PRIMER_RIGHT_40_SEQUENCE=AGGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_40=302,22
PRIMER_RIGHT_40=1212,21
PRIMER_LEFT_40_TM=59.592
PRIMER_RIGHT_40_TM=59.856
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=911
PRIMER_PAIR_41_PENALTY=3.552399
PRIMER_LEFT_41_PENALTY=2.021762
PRIMER_RIGHT_41_PENALTY=1.530636
PRIMER_LEFT_41_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_41_SEQUENCE=AATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_41=609,21
PRIMER_RIGHT_41=1208,20
PRIMER_LEFT_41_TM=58.978
PRIMER_RIGHT_41_TM=58.469
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=600
PRIMER_PAIR_42_PENALTY=3.560136
PRIMER_LEFT_42_PENALTY=2.058853
PRIMER_RIGHT_42_PENALTY=1.501283
PRIMER_LEFT_42_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_42_SEQUENCE=AGGAAATCTTGGTCGGTTGT
PRIMER_LEFT_42=609,22
PRIMER_RIGHT_42=1212,20
PRIMER_LEFT_42_TM=59.941
PRIMER_RIGHT_42_TM=58.499
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=604
PRIMER_PAIR_43_PENALTY=3.589490
PRIMER_LEFT_43_PENALTY=2.058853
PRIMER_RIGHT_43_PENALTY=1.530636
PRIMER_LEFT_43_SEQUENCE=CACATGTTAGGCAAAGCAGAAG
PRIMER_RIGHT_43_SEQUENCE=AATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_43=609,22
PRIMER_RIGHT_43=1208,20
PRIMER_LEFT_43_TM=59.941
PRIMER_RIGHT_43_TM=58.469
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=600
PRIMER_PAIR_44_PENALTY=3.607483
PRIMER_LEFT_44_PENALTY=2.493925
PRIMER_RIGHT_44_PENALTY=1.113558
PRIMER_LEFT_44_SEQUENCE=CACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_44_SEQUENCE=GGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_44=609,20
PRIMER_RIGHT_44=1211,20
PRIMER_LEFT_44_TM=57.506
PRIMER_RIGHT_44_TM=58.886
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=603
PRIMER_PAIR_45_PENALTY=3.610569
PRIMER_LEFT_45_PENALTY=2.493925
PRIMER_RIGHT_45_PENALTY=1.116644
PRIMER_LEFT_45_SEQUENCE=CACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_45_SEQUENCE=AAATCTTGGTCGGTTGTTGTG
PRIMER_LEFT_45=609,20
PRIMER_RIGHT_45=1209,21
PRIMER_LEFT_45_TM=57.506
PRIMER_RIGHT_45_TM=59.883
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=601
PRIMER_PAIR_46_PENALTY=3.631195
PRIMER_LEFT_46_PENALTY=3.025372
PRIMER_RIGHT_46_PENALTY=0.605823
PRIMER_LEFT_46_SEQUENCE=AACACATGTTAGGCAAAGCAG
PRIMER_RIGHT_46_SEQUENCE=TCTTGGTCGGTTGTTGTGTG
PRIMER_LEFT_46=607,21
PRIMER_RIGHT_46=1206,20
PRIMER_LEFT_46_TM=57.975
PRIMER_RIGHT_46_TM=60.606
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=600
PRIMER_PAIR_47_PENALTY=3.637676
PRIMER_LEFT_47_PENALTY=2.493925
PRIMER_RIGHT_47_PENALTY=1.143751
PRIMER_LEFT_47_SEQUENCE=CACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_47_SEQUENCE=AGGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_47=609,20
PRIMER_RIGHT_47=1212,21
PRIMER_LEFT_47_TM=57.506
PRIMER_RIGHT_47_TM=59.856
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=604
PRIMER_PAIR_48_PENALTY=3.654767
PRIMER_LEFT_48_PENALTY=2.021762
PRIMER_RIGHT_48_PENALTY=1.633005
PRIMER_LEFT_48_SEQUENCE=CACATGTTAGGCAAAGCAGAA
PRIMER_RIGHT_48_SEQUENCE=AATCTTGGTCGGTTGTTGTGT
PRIMER_LEFT_48=609,21
PRIMER_RIGHT_48=1208,21
PRIMER_LEFT_48_TM=58.978
PRIMER_RIGHT_48_TM=59.367
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=600
PRIMER_PAIR_49_PENALTY=3.667749
PRIMER_LEFT_49_PENALTY=2.554191
PRIMER_RIGHT_49_PENALTY=1.113558
PRIMER_LEFT_49_SEQUENCE=ACACATGTTAGGCAAAGCAGA
PRIMER_RIGHT_49_SEQUENCE=GGAAATCTTGGTCGGTTGTT
PRIMER_LEFT_49=608,21
PRIMER_RIGHT_49=1211,20
PRIMER_LEFT_49_TM=58.446
PRIMER_RIGHT_49_TM=58.886
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=604
=
SEQUENCE_ID=MAGIC.329_2_5933179_5933354_zu-0_hi-0_93736_131209_TRUE
SEQUENCE_TARGET=950,275
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTAGTTTTAAAGTGGCNTTTTTTTNGTGTGGTTCTAGNGTNTNTTNAGTNATTGGNTNTNCTATACANNANATCNTTANNNNNNNNNNNNNNNNNNNNNNCNATANAATGAAANATATACNNTANCNTTTNAAAGAATGAAAAAATAAATTAANAGACAGTTTCGNAGAAGANTTTTTTNNNNNNNNNNNAANTANATGANANATATGAAGAGATTAGTGNCGTGAAGGCNACTCATGGTTTGCNTCTTCTTCTTTAGTTTGTTGCTTTGTTTCAGCATTNGCCACTTTTTTGTCTATCAAAGGCTTTATCATTGCCTCCAAGTCATCTATATCTTGTGTGACTTTTTCCACCTGCNATTTNTNNGTTTTGACTTAGTCAATCACANTCANANNTNTATATATGTTTGNNNATTATTNGGTTCCAAAANATCCCCCAAATGATAAACCAATAAAAGNNACCCAAANANATANATCATATNTTCAAAATGTAATCNTTTACNTTAGCCTTTGTTAGTGTTNNNNNNNNNNNAGATTTTTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNATNAAGTGTNGTGGTNTACCTTGTGAAGGAAATCTTGGGTTANATGTGCTTCATGGGCAGCAACCTCTGAAATNTGTTCTAGAACCAAAGCCACTTGTTTTAGTTTANTTTTCTCAGGCAGTTTCTCTGCCATTTCTTCAGCCATTTCGTCAGTCGCNGTNGCTACTTTCTCCACCATTTCNNCTACAGCCTCTACCCCTTCCACAACCAACTCCGCCTCTCCTGCANNCANNNNATCATTCNANTTNNNNNNGTNCTNNNNNNANNNNANNNNNNNNNNNNNNNNNANNANNNNNNTANNANTNNNANACANNANNNNANCNTANANTNNNATNNAAGNNNNTTNTAGNNAANNNNNATGNNAGNTNTNANANNTNNATTACCTTCNATNCGTTTNAGTTTTTGNANCCTTTNGTTGTTCCAGAAAGATAACACTAANGATATCGCAGAACCTATAACCCATCTTCCCCTGCCNNTTANTCTCCANANNNNANNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNNNNNNNNNNNNNNNNNNACCANTGGTTTGGAGGAGANGGTTNCATCTTGGAGTCANTNGAAACATTACTNAAGTATCTCCCGGCATTANGNGGATTTGATAACTTGAGACCTTGTGGANANATTTGGTTATGATAGTCTCTTCCAAATCGAATAATCGNNGGACATGGATTCANANNTGTNTTCCTCTCNACTTTGTTNTATATTTGTAAGACNTCNGCAAGGCCCTGNCNATACACCGCAAAACCTCCGCTCNGCATTCTCCGACTTAGATACATNNTCAATCNGTTTTATNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNATTCAGTTTNTGTGTGANATTGGTTAAGCGTNTGNTTAAGATANAAGGCTTTTANGTGTAGTAANGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAAATNNNNNNNNNNNNNNNNAACAGAATTTACTTCAAAANGCNATTGAATTAAATAGGAAANCNTCNTTAAAAAATTNCATATTGNAANNTGGANNTATNNNNNNNNNNNATATTAGAATAAATAATTTTCAGCATNTTCNTCAAAGATTTTTTTAAAATAAAAATTAAATAAATTGACATTTTCTTCCTTTCTGGACATAAACCTAAATTAAGGAACATAATATTGAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=5.286571
PRIMER_LEFT_0_PENALTY=0.012411
PRIMER_RIGHT_0_PENALTY=5.274160
PRIMER_LEFT_0_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_0_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_0=543,20
PRIMER_RIGHT_0=1410,25
PRIMER_LEFT_0_TM=59.988
PRIMER_RIGHT_0_TM=60.274
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=868
PRIMER_PAIR_1_PENALTY=5.286571
PRIMER_LEFT_1_PENALTY=0.012411
PRIMER_RIGHT_1_PENALTY=5.274160
PRIMER_LEFT_1_SEQUENCE=TCCCCCAAATGATAAACCAA
PRIMER_RIGHT_1_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_1=543,20
PRIMER_RIGHT_1=1411,25
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=60.274
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=869
PRIMER_PAIR_2_PENALTY=5.314932
PRIMER_LEFT_2_PENALTY=0.040772
PRIMER_RIGHT_2_PENALTY=5.274160
PRIMER_LEFT_2_SEQUENCE=CTCTACCCCTTCCACAACCA
PRIMER_RIGHT_2_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_2=872,20
PRIMER_RIGHT_2=1410,25
PRIMER_LEFT_2_TM=59.959
PRIMER_RIGHT_2_TM=60.274
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=539
PRIMER_PAIR_3_PENALTY=5.314932
PRIMER_LEFT_3_PENALTY=0.040772
PRIMER_RIGHT_3_PENALTY=5.274160
PRIMER_LEFT_3_SEQUENCE=CTCTACCCCTTCCACAACCA
PRIMER_RIGHT_3_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_3=872,20
PRIMER_RIGHT_3=1411,25
PRIMER_LEFT_3_TM=59.959
PRIMER_RIGHT_3_TM=60.274
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=540
PRIMER_PAIR_4_PENALTY=5.454394
PRIMER_LEFT_4_PENALTY=0.180233
PRIMER_RIGHT_4_PENALTY=5.274160
PRIMER_LEFT_4_SEQUENCE=GGCAGTTTCTCTGCCATTTC
PRIMER_RIGHT_4_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_4=798,20
PRIMER_RIGHT_4=1410,25
PRIMER_LEFT_4_TM=59.820
PRIMER_RIGHT_4_TM=60.274
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=613
PRIMER_PAIR_5_PENALTY=5.454394
PRIMER_LEFT_5_PENALTY=0.180233
PRIMER_RIGHT_5_PENALTY=5.274160
PRIMER_LEFT_5_SEQUENCE=GGCAGTTTCTCTGCCATTTC
PRIMER_RIGHT_5_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_5=798,20
PRIMER_RIGHT_5=1411,25
PRIMER_LEFT_5_TM=59.820
PRIMER_RIGHT_5_TM=60.274
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=614
PRIMER_PAIR_6_PENALTY=5.487666
PRIMER_LEFT_6_PENALTY=0.213506
PRIMER_RIGHT_6_PENALTY=5.274160
PRIMER_LEFT_6_SEQUENCE=CTGCCATTTCTTCAGCCATT
PRIMER_RIGHT_6_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_6=808,20
PRIMER_RIGHT_6=1410,25
PRIMER_LEFT_6_TM=60.214
PRIMER_RIGHT_6_TM=60.274
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=603
PRIMER_PAIR_7_PENALTY=5.487666
PRIMER_LEFT_7_PENALTY=0.213506
PRIMER_RIGHT_7_PENALTY=5.274160
PRIMER_LEFT_7_SEQUENCE=CTGCCATTTCTTCAGCCATT
PRIMER_RIGHT_7_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_7=808,20
PRIMER_RIGHT_7=1411,25
PRIMER_LEFT_7_TM=60.214
PRIMER_RIGHT_7_TM=60.274
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=604
PRIMER_PAIR_8_PENALTY=5.586170
PRIMER_LEFT_8_PENALTY=0.312010
PRIMER_RIGHT_8_PENALTY=5.274160
PRIMER_LEFT_8_SEQUENCE=TACAGCCTCTACCCCTTCCA
PRIMER_RIGHT_8_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_8=866,20
PRIMER_RIGHT_8=1410,25
PRIMER_LEFT_8_TM=59.688
PRIMER_RIGHT_8_TM=60.274
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=545
PRIMER_PAIR_9_PENALTY=5.586170
PRIMER_LEFT_9_PENALTY=0.312010
PRIMER_RIGHT_9_PENALTY=5.274160
PRIMER_LEFT_9_SEQUENCE=TACAGCCTCTACCCCTTCCA
PRIMER_RIGHT_9_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_9=866,20
PRIMER_RIGHT_9=1411,25
PRIMER_LEFT_9_TM=59.688
PRIMER_RIGHT_9_TM=60.274
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=546
PRIMER_PAIR_10_PENALTY=5.606189
PRIMER_LEFT_10_PENALTY=0.332028
PRIMER_RIGHT_10_PENALTY=5.274160
PRIMER_LEFT_10_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_10_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_10=414,20
PRIMER_RIGHT_10=1410,25
PRIMER_LEFT_10_TM=59.668
PRIMER_RIGHT_10_TM=60.274
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=997
PRIMER_PAIR_11_PENALTY=5.606189
PRIMER_LEFT_11_PENALTY=0.332028
PRIMER_RIGHT_11_PENALTY=5.274160
PRIMER_LEFT_11_SEQUENCE=AGGCTTTATCATTGCCTCCA
PRIMER_RIGHT_11_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_11=414,20
PRIMER_RIGHT_11=1411,25
PRIMER_LEFT_11_TM=59.668
PRIMER_RIGHT_11_TM=60.274
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=998
PRIMER_PAIR_12_PENALTY=5.618709
PRIMER_LEFT_12_PENALTY=0.344549
PRIMER_RIGHT_12_PENALTY=5.274160
PRIMER_LEFT_12_SEQUENCE=TCTACCCCTTCCACAACCAA
PRIMER_RIGHT_12_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_12=873,20
PRIMER_RIGHT_12=1410,25
PRIMER_LEFT_12_TM=60.345
PRIMER_RIGHT_12_TM=60.274
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=538
PRIMER_PAIR_13_PENALTY=5.618709
PRIMER_LEFT_13_PENALTY=0.344549
PRIMER_RIGHT_13_PENALTY=5.274160
PRIMER_LEFT_13_SEQUENCE=TCTACCCCTTCCACAACCAA
PRIMER_RIGHT_13_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_13=873,20
PRIMER_RIGHT_13=1411,25
PRIMER_LEFT_13_TM=60.345
PRIMER_RIGHT_13_TM=60.274
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=539
PRIMER_PAIR_14_PENALTY=5.623462
PRIMER_LEFT_14_PENALTY=0.349302
PRIMER_RIGHT_14_PENALTY=5.274160
PRIMER_LEFT_14_SEQUENCE=GCCATTTCTTCAGCCATTTC
PRIMER_RIGHT_14_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_14=810,20
PRIMER_RIGHT_14=1410,25
PRIMER_LEFT_14_TM=59.651
PRIMER_RIGHT_14_TM=60.274
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=601
PRIMER_PAIR_15_PENALTY=5.623462
PRIMER_LEFT_15_PENALTY=0.349302
PRIMER_RIGHT_15_PENALTY=5.274160
PRIMER_LEFT_15_SEQUENCE=GCCATTTCTTCAGCCATTTC
PRIMER_RIGHT_15_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_15=810,20
PRIMER_RIGHT_15=1411,25
PRIMER_LEFT_15_TM=59.651
PRIMER_RIGHT_15_TM=60.274
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=602
PRIMER_PAIR_16_PENALTY=5.671104
PRIMER_LEFT_16_PENALTY=0.396944
PRIMER_RIGHT_16_PENALTY=5.274160
PRIMER_LEFT_16_SEQUENCE=CAGGCAGTTTCTCTGCCATT
PRIMER_RIGHT_16_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_16=796,20
PRIMER_RIGHT_16=1410,25
PRIMER_LEFT_16_TM=60.397
PRIMER_RIGHT_16_TM=60.274
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=615
PRIMER_PAIR_17_PENALTY=5.671104
PRIMER_LEFT_17_PENALTY=0.396944
PRIMER_RIGHT_17_PENALTY=5.274160
PRIMER_LEFT_17_SEQUENCE=CAGGCAGTTTCTCTGCCATT
PRIMER_RIGHT_17_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_17=796,20
PRIMER_RIGHT_17=1411,25
PRIMER_LEFT_17_TM=60.397
PRIMER_RIGHT_17_TM=60.274
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=616
PRIMER_PAIR_18_PENALTY=5.756822
PRIMER_LEFT_18_PENALTY=0.482662
PRIMER_RIGHT_18_PENALTY=5.274160
PRIMER_LEFT_18_SEQUENCE=TCTCTGCCATTTCTTCAGCC
PRIMER_RIGHT_18_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_18=805,20
PRIMER_RIGHT_18=1410,25
PRIMER_LEFT_18_TM=60.483
PRIMER_RIGHT_18_TM=60.274
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=606
PRIMER_PAIR_19_PENALTY=5.756822
PRIMER_LEFT_19_PENALTY=0.482662
PRIMER_RIGHT_19_PENALTY=5.274160
PRIMER_LEFT_19_SEQUENCE=TCTCTGCCATTTCTTCAGCC
PRIMER_RIGHT_19_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_19=805,20
PRIMER_RIGHT_19=1411,25
PRIMER_LEFT_19_TM=60.483
PRIMER_RIGHT_19_TM=60.274
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=607
PRIMER_PAIR_20_PENALTY=5.784791
PRIMER_LEFT_20_PENALTY=0.510631
PRIMER_RIGHT_20_PENALTY=5.274160
PRIMER_LEFT_20_SEQUENCE=ACAGCCTCTACCCCTTCCAC
PRIMER_RIGHT_20_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_20=867,20
PRIMER_RIGHT_20=1410,25
PRIMER_LEFT_20_TM=60.511
PRIMER_RIGHT_20_TM=60.274
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=544
PRIMER_PAIR_21_PENALTY=5.784791
PRIMER_LEFT_21_PENALTY=0.510631
PRIMER_RIGHT_21_PENALTY=5.274160
PRIMER_LEFT_21_SEQUENCE=ACAGCCTCTACCCCTTCCAC
PRIMER_RIGHT_21_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_21=867,20
PRIMER_RIGHT_21=1411,25
PRIMER_LEFT_21_TM=60.511
PRIMER_RIGHT_21_TM=60.274
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=545
PRIMER_PAIR_22_PENALTY=5.859363
PRIMER_LEFT_22_PENALTY=0.585203
PRIMER_RIGHT_22_PENALTY=5.274160
PRIMER_LEFT_22_SEQUENCE=TGCCATTTCTTCAGCCATTT
PRIMER_RIGHT_22_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_22=809,20
PRIMER_RIGHT_22=1410,25
PRIMER_LEFT_22_TM=60.585
PRIMER_RIGHT_22_TM=60.274
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.2000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=602
PRIMER_PAIR_23_PENALTY=5.859363
PRIMER_LEFT_23_PENALTY=0.585203
PRIMER_RIGHT_23_PENALTY=5.274160
PRIMER_LEFT_23_SEQUENCE=TGCCATTTCTTCAGCCATTT
PRIMER_RIGHT_23_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_23=809,20
PRIMER_RIGHT_23=1411,25
PRIMER_LEFT_23_TM=60.585
PRIMER_RIGHT_23_TM=60.274
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.2000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=603
PRIMER_PAIR_24_PENALTY=5.878920
PRIMER_LEFT_24_PENALTY=0.604759
PRIMER_RIGHT_24_PENALTY=5.274160
PRIMER_LEFT_24_SEQUENCE=TTTCTTCAGCCATTTCGTCA
PRIMER_RIGHT_24_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_24=814,20
PRIMER_RIGHT_24=1410,25
PRIMER_LEFT_24_TM=59.395
PRIMER_RIGHT_24_TM=60.274
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=597
PRIMER_PAIR_25_PENALTY=5.878920
PRIMER_LEFT_25_PENALTY=0.604759
PRIMER_RIGHT_25_PENALTY=5.274160
PRIMER_LEFT_25_SEQUENCE=TTTCTTCAGCCATTTCGTCA
PRIMER_RIGHT_25_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_25=814,20
PRIMER_RIGHT_25=1411,25
PRIMER_LEFT_25_TM=59.395
PRIMER_RIGHT_25_TM=60.274
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=598
PRIMER_PAIR_26_PENALTY=5.970110
PRIMER_LEFT_26_PENALTY=0.695950
PRIMER_RIGHT_26_PENALTY=5.274160
PRIMER_LEFT_26_SEQUENCE=TACCCCTTCCACAACCAACT
PRIMER_RIGHT_26_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_26=875,20
PRIMER_RIGHT_26=1410,25
PRIMER_LEFT_26_TM=59.304
PRIMER_RIGHT_26_TM=60.274
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=536
PRIMER_PAIR_27_PENALTY=5.970110
PRIMER_LEFT_27_PENALTY=0.695950
PRIMER_RIGHT_27_PENALTY=5.274160
PRIMER_LEFT_27_SEQUENCE=TACCCCTTCCACAACCAACT
PRIMER_RIGHT_27_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_27=875,20
PRIMER_RIGHT_27=1411,25
PRIMER_LEFT_27_TM=59.304
PRIMER_RIGHT_27_TM=60.274
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=537
PRIMER_PAIR_28_PENALTY=5.970110
PRIMER_LEFT_28_PENALTY=0.695950
PRIMER_RIGHT_28_PENALTY=5.274160
PRIMER_LEFT_28_SEQUENCE=CTACCCCTTCCACAACCAAC
PRIMER_RIGHT_28_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_28=874,20
PRIMER_RIGHT_28=1410,25
PRIMER_LEFT_28_TM=59.304
PRIMER_RIGHT_28_TM=60.274
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=537
PRIMER_PAIR_29_PENALTY=5.970110
PRIMER_LEFT_29_PENALTY=0.695950
PRIMER_RIGHT_29_PENALTY=5.274160
PRIMER_LEFT_29_SEQUENCE=CTACCCCTTCCACAACCAAC
PRIMER_RIGHT_29_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_29=874,20
PRIMER_RIGHT_29=1411,25
PRIMER_LEFT_29_TM=59.304
PRIMER_RIGHT_29_TM=60.274
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=538
PRIMER_PAIR_30_PENALTY=6.009849
PRIMER_LEFT_30_PENALTY=0.735689
PRIMER_RIGHT_30_PENALTY=5.274160
PRIMER_LEFT_30_SEQUENCE=CCTCTACCCCTTCCACAACC
PRIMER_RIGHT_30_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_30=871,20
PRIMER_RIGHT_30=1410,25
PRIMER_LEFT_30_TM=60.736
PRIMER_RIGHT_30_TM=60.274
PRIMER_LEFT_30_GC_PERCENT=60.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=540
PRIMER_PAIR_31_PENALTY=6.009849
PRIMER_LEFT_31_PENALTY=0.735689
PRIMER_RIGHT_31_PENALTY=5.274160
PRIMER_LEFT_31_SEQUENCE=CCTCTACCCCTTCCACAACC
PRIMER_RIGHT_31_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_31=871,20
PRIMER_RIGHT_31=1411,25
PRIMER_LEFT_31_TM=60.736
PRIMER_RIGHT_31_TM=60.274
PRIMER_LEFT_31_GC_PERCENT=60.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=541
PRIMER_PAIR_32_PENALTY=6.156778
PRIMER_LEFT_32_PENALTY=0.882618
PRIMER_RIGHT_32_PENALTY=5.274160
PRIMER_LEFT_32_SEQUENCE=GCCTCTACCCCTTCCACAAC
PRIMER_RIGHT_32_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_32=870,20
PRIMER_RIGHT_32=1410,25
PRIMER_LEFT_32_TM=60.883
PRIMER_RIGHT_32_TM=60.274
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=541
PRIMER_PAIR_33_PENALTY=6.156778
PRIMER_LEFT_33_PENALTY=0.882618
PRIMER_RIGHT_33_PENALTY=5.274160
PRIMER_LEFT_33_SEQUENCE=GCCTCTACCCCTTCCACAAC
PRIMER_RIGHT_33_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_33=870,20
PRIMER_RIGHT_33=1411,25
PRIMER_LEFT_33_TM=60.883
PRIMER_RIGHT_33_TM=60.274
PRIMER_LEFT_33_GC_PERCENT=60.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=542
PRIMER_PAIR_34_PENALTY=6.275592
PRIMER_LEFT_34_PENALTY=1.001431
PRIMER_RIGHT_34_PENALTY=5.274160
PRIMER_LEFT_34_SEQUENCE=TTCTTCAGCCATTTCGTCAG
PRIMER_RIGHT_34_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_34=815,20
PRIMER_RIGHT_34=1410,25
PRIMER_LEFT_34_TM=58.999
PRIMER_RIGHT_34_TM=60.274
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.1000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=596
PRIMER_PAIR_35_PENALTY=6.275592
PRIMER_LEFT_35_PENALTY=1.001431
PRIMER_RIGHT_35_PENALTY=5.274160
PRIMER_LEFT_35_SEQUENCE=TTCTTCAGCCATTTCGTCAG
PRIMER_RIGHT_35_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_35=815,20
PRIMER_RIGHT_35=1411,25
PRIMER_LEFT_35_TM=58.999
PRIMER_RIGHT_35_TM=60.274
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=597
PRIMER_PAIR_36_PENALTY=6.281837
PRIMER_LEFT_36_PENALTY=1.007677
PRIMER_RIGHT_36_PENALTY=5.274160
PRIMER_LEFT_36_SEQUENCE=AGCCTCTACCCCTTCCACAA
PRIMER_RIGHT_36_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_36=869,20
PRIMER_RIGHT_36=1410,25
PRIMER_LEFT_36_TM=61.008
PRIMER_RIGHT_36_TM=60.274
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.1000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=542
PRIMER_PAIR_37_PENALTY=6.281837
PRIMER_LEFT_37_PENALTY=1.007677
PRIMER_RIGHT_37_PENALTY=5.274160
PRIMER_LEFT_37_SEQUENCE=AGCCTCTACCCCTTCCACAA
PRIMER_RIGHT_37_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_37=869,20
PRIMER_RIGHT_37=1411,25
PRIMER_LEFT_37_TM=61.008
PRIMER_RIGHT_37_TM=60.274
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=543
PRIMER_PAIR_38_PENALTY=6.337666
PRIMER_LEFT_38_PENALTY=1.063505
PRIMER_RIGHT_38_PENALTY=5.274160
PRIMER_LEFT_38_SEQUENCE=AGGCAGTTTCTCTGCCATTT
PRIMER_RIGHT_38_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_38=797,20
PRIMER_RIGHT_38=1410,25
PRIMER_LEFT_38_TM=58.936
PRIMER_RIGHT_38_TM=60.274
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.2000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=614
PRIMER_PAIR_39_PENALTY=6.337666
PRIMER_LEFT_39_PENALTY=1.063505
PRIMER_RIGHT_39_PENALTY=5.274160
PRIMER_LEFT_39_SEQUENCE=AGGCAGTTTCTCTGCCATTT
PRIMER_RIGHT_39_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_39=797,20
PRIMER_RIGHT_39=1411,25
PRIMER_LEFT_39_TM=58.936
PRIMER_RIGHT_39_TM=60.274
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=615
PRIMER_PAIR_40_PENALTY=6.406878
PRIMER_LEFT_40_PENALTY=1.132718
PRIMER_RIGHT_40_PENALTY=5.274160
PRIMER_LEFT_40_SEQUENCE=TTCTTCAGCCATTTCGTCAGT
PRIMER_RIGHT_40_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_40=815,21
PRIMER_RIGHT_40=1410,25
PRIMER_LEFT_40_TM=59.867
PRIMER_RIGHT_40_TM=60.274
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=596
PRIMER_PAIR_41_PENALTY=6.406878
PRIMER_LEFT_41_PENALTY=1.132718
PRIMER_RIGHT_41_PENALTY=5.274160
PRIMER_LEFT_41_SEQUENCE=TTCTTCAGCCATTTCGTCAGT
PRIMER_RIGHT_41_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_41=815,21
PRIMER_RIGHT_41=1411,25
PRIMER_LEFT_41_TM=59.867
PRIMER_RIGHT_41_TM=60.274
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=597
PRIMER_PAIR_42_PENALTY=6.454177
PRIMER_LEFT_42_PENALTY=1.180017
PRIMER_RIGHT_42_PENALTY=5.274160
PRIMER_LEFT_42_SEQUENCE=ACCCCTTCCACAACCAACTC
PRIMER_RIGHT_42_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_42=876,20
PRIMER_RIGHT_42=1410,25
PRIMER_LEFT_42_TM=61.180
PRIMER_RIGHT_42_TM=60.274
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=8.1000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=535
PRIMER_PAIR_43_PENALTY=6.454177
PRIMER_LEFT_43_PENALTY=1.180017
PRIMER_RIGHT_43_PENALTY=5.274160
PRIMER_LEFT_43_SEQUENCE=ACCCCTTCCACAACCAACTC
PRIMER_RIGHT_43_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_43=876,20
PRIMER_RIGHT_43=1411,25
PRIMER_LEFT_43_TM=61.180
PRIMER_RIGHT_43_TM=60.274
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=536
PRIMER_PAIR_44_PENALTY=6.540826
PRIMER_LEFT_44_PENALTY=1.266666
PRIMER_RIGHT_44_PENALTY=5.274160
PRIMER_LEFT_44_SEQUENCE=CTACCCCTTCCACAACCAACT
PRIMER_RIGHT_44_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_44=874,21
PRIMER_RIGHT_44=1410,25
PRIMER_LEFT_44_TM=60.267
PRIMER_RIGHT_44_TM=60.274
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=8.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=537
PRIMER_PAIR_45_PENALTY=6.540826
PRIMER_LEFT_45_PENALTY=1.266666
PRIMER_RIGHT_45_PENALTY=5.274160
PRIMER_LEFT_45_SEQUENCE=CTACCCCTTCCACAACCAACT
PRIMER_RIGHT_45_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_45=874,21
PRIMER_RIGHT_45=1411,25
PRIMER_LEFT_45_TM=60.267
PRIMER_RIGHT_45_TM=60.274
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=538
PRIMER_PAIR_46_PENALTY=6.574985
PRIMER_LEFT_46_PENALTY=1.300825
PRIMER_RIGHT_46_PENALTY=5.274160
PRIMER_LEFT_46_SEQUENCE=CATTTCTTCAGCCATTTCGTC
PRIMER_RIGHT_46_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_46=812,21
PRIMER_RIGHT_46=1410,25
PRIMER_LEFT_46_TM=59.699
PRIMER_RIGHT_46_TM=60.274
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.1000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=599
PRIMER_PAIR_47_PENALTY=6.574985
PRIMER_LEFT_47_PENALTY=1.300825
PRIMER_RIGHT_47_PENALTY=5.274160
PRIMER_LEFT_47_SEQUENCE=CATTTCTTCAGCCATTTCGTC
PRIMER_RIGHT_47_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_47=812,21
PRIMER_RIGHT_47=1411,25
PRIMER_LEFT_47_TM=59.699
PRIMER_RIGHT_47_TM=60.274
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=600
PRIMER_PAIR_48_PENALTY=6.645728
PRIMER_LEFT_48_PENALTY=1.371568
PRIMER_RIGHT_48_PENALTY=5.274160
PRIMER_LEFT_48_SEQUENCE=TTTCTTCAGCCATTTCGTCAG
PRIMER_RIGHT_48_SEQUENCE=CACAAGGTCTCAAGTTATCAAATCC
PRIMER_LEFT_48=814,21
PRIMER_RIGHT_48=1410,25
PRIMER_LEFT_48_TM=60.372
PRIMER_RIGHT_48_TM=60.274
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=597
PRIMER_PAIR_49_PENALTY=6.645728
PRIMER_LEFT_49_PENALTY=1.371568
PRIMER_RIGHT_49_PENALTY=5.274160
PRIMER_LEFT_49_SEQUENCE=TTTCTTCAGCCATTTCGTCAG
PRIMER_RIGHT_49_SEQUENCE=CCACAAGGTCTCAAGTTATCAAATC
PRIMER_LEFT_49=814,21
PRIMER_RIGHT_49=1411,25
PRIMER_LEFT_49_TM=60.372
PRIMER_RIGHT_49_TM=60.274
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=598
=
SEQUENCE_ID=MAGIC.105_4_18391843_18392143_oy-0_no-0_75103_66569_TRUE
SEQUENCE_TARGET=950,400
SEQUENCE_TEMPLATE=TACGTAATTTGAAAAATAGTCTTTTGCATNGCCTTTTAATCAATTTTCAAACCCATCTTTCTCCTATTTCTTTCACACACACAACAAAAAACTAAAATACCAAACTGATTGTGTTCAGATTCCCGGAACATGCCTGCCCTGCATACCAAGAACAGTAGTAAACCAGTAAAAGTGACGAACATCTAGAGCANAAATCACTAAGATAGGCCAAAACACTTNCTTGTAATTAATGTGATTCATTGATCATATAGCCACATGACATGAGATGATACGTTTTAAAATGGGACTATTTGAAGTAATGAGCAATGCTGTTCCGAAGATGGTTTAGACAAAAGCTCTTTCACATTGATCTCNAAAAAAAAAAAGAGGTAAACGGTTTATATGATATTATGATGAAACATCCGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACATTCTGAAACTCACAATTTTAAAACTGAAAGAAANACCTTAAAATGTATAAAGCAGTGTTTTGGTTAATTACTTTGATTTTTCTCTTGTTTTCTGGATCATCAAACACAGCATTAGCCCGAANNGCATNTGAAACACCCANCACAAGTAAGATTACAATCTGAGAAATTATATAGTTATGTTTCTTACATGGCCATACTTTGGTGGGATANTACATCTATNATAGAGATCAANTTGNCGCGAATATATTTCTCAGCNAAATAAATTTGAATAACCTAATTGATCATTATTACATTGTTGNNNNNNNNNNNNNNNNNAAAACAGAAGAGGGACAATGGGAAGAAAAGGTGATAAGAGGGAGCAAAATTGAAACACCAGGNAGCATTTCAAGGCGGGCACCTGANCCACCGACAAACCCCAAACCCTANTGATGATCNTATTNAAAGCTTANTCNNTNATGNTGTTACNTAGCTCAGATGCGGAATAAAAAANCTNTTATTCGCAAANTAATGTTTATTTTGTCTTCTCNCGTATATATATCATCTTCACACGGGCCTGTCAANAAANAAAAAAAANNNNNANNATNTNTTTTTTTTTTNTGTNAGGCCAANCAAAAGATATANATCTATGGAATCTAATAGATACATAATACATGTACCGGATTNGGCTCAGAAAACCAACAGTTGAAGTATNTATAATTCNTCTACAATGNGTTTGAGTCTTGTCGNGTACGTTTATGTTTNGATTTGNTCAAAACCAGTATCGCTTAACCNAAATCGAACCGCATTATAATTNTAGTAAACANATCAATCTCATTATTTTGATGGCAAATAAATAAACTGAAACTGAAATCANNGTAACAACTTAATAAATGAAAATCCATCAATAAGATCTTTTGCTAGAATTNAAACAATATTGAGATTAATAAAAGATTGANTTAAATAAGATCTTGTCTTTCAAACNTTAGAAACAANAGGTNACTCNCTCGTTAAGATTTTTCTCTCNAAGANANCCTTAAAACGCATCTTCTTCGTTTGACCGTCTCTAAGAGTCTTACCAGACGAGAACATGGACTATTCTTCAAAAAGANTAGATAACGCATGCAAATTAAAAGAGTTGGGGACGACTTTTTCTTCGTTAGAGAAACCTTATNGTATGACTATAAATACACCTTCTCTCCAAAAGAAATCAAAAATCATTTTCTAAGACAAATACAAATTGACATAGACCAAAATATTTGGCTTATGAACGATTTATNCATTGTAAAACTCATTATTTAATTAAAANCAAANNNTATCACCGCCTGGTTTGGTTAATTGCTTTGATTGTTCTATTGTTCGTTGGATCATCAAACATAGAACAAGTAAGTTTCTAGTTATTGNTAAACTTTATTCATTTCTCAAACCAAATTAAAGTTATTTATTTTATTNATATATTNATN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_15563493_15563573_oy-0_no-0_7760_765027_TRUE
SEQUENCE_TARGET=950,180
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNCNANNATATTNAGTTGNGTCGTTAATGCGATGCTAATTCCACNCTAATTACATGNAATTTAGCGACACATTTATTTCCANGCAATATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACATATGCTCATAACTGCANCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_14038028_14038303_no-0_ler-0_4084_762_TRUE
SEQUENCE_TARGET=950,375
SEQUENCE_TEMPLATE=GGGANAGCCCCNAATTAAAGTAGATACAACCACTAGGAATCCGTAATCTTCTCGGGGTCAACGATAGCCNGATCGGCTTCGGTTGGNTCAACAACAGCTGACTCAGTTTTGGTTGGTTCACNGGCAGAGAGTAGCTTAGAAGAGTTTCCCGGCTCAGTTCCGGCTTCATGTTCAACATCCGGGATGATCACTGTCTCACTNTTCACGANCGGACAGTAGTATCGACCANTTCACTAGAGCCTGAAGNGAACGTTTCAAGTCAGCAANAAACACAAATACAACAACTNNNGNNNATAANGNTNCAGATNTAAAAGNTTANCANCCCAATCTAAGCCTGATTCACTCAAANNCANNGNNNNGNNNTAANANTCCTCGAAGTGTTGAGGGTTAAAGGACAAGGTGGGAAGAGNAGCAAGGTGGCCTCTTTTCTCAGCTAAAACAGCCTCCAGTTTGGACCTTTCTTGTTCCTCAGAGAAATCTTCCTCAAGGGTTTGGTCCAACANAAGCAGGNTNCTTTCGTGCTNNTNNNNNNNNNNNTNNAGCTCCGATNTGGCNNTCTCNGTTTGAATGANNAGGATAGCNCCTTGNATCANNTCCATNCCGCGACNTTTCACNTCTCTTNNNNNNNNNNNNNNNNNNNTGGCTAATTCAGCATCNNANNNNNNNNNNNNNNNNTTGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNAGTCGAGAGTTCNTCCTNNAGCTTGTCNTANNTTTGNTACTGATCNTGCAACTCGCCATTNGANNTCATGAGCTTNNNNNNNAGATGNTCGNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAGAGGAGGAGCNNNNNNNNNNNNNNNGNGAAGANAANTTGCCTTTCCAAGCATCNATNTCCNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNGATCATGTNNTTAAGCTCNNTGTTCGCCTNAGCAANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACCCGANATGAATGATGNGCNTGGAGTAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGATNGTGTNTTTCCNNGTTTTGAAGCNNNGNAANACGTTGCCCTCTTTCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCGAGNGAAATNAANCCNTNGTTNGAACCTNTAAGCNATTTNGTNTGAGGAACGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTNGATCAGNCTTCTCNCCACTAGGTNGNNGAGAAGGNNNNNNNNNNNNNNNNGAGGAGGAGNACCCATCGATCNAGATGGAGGAGATGATCGNNGAGAGCGATCAGTNCGAGCCNTTGACTTTTCCCTCCAAGGAGAAGAGCTGTTNCGAGCTGGTCTCTCNCTCNNTNTNNNNATNAAGTCAGCGATTGGCTTGCTGCTCAGTCCATCACGGGCTNAAGTCCCGTGTGGTNTGGGAGGANGNGGTGGAAGATGATGGAGTTGTCCAGGAGGACTCATNTNGAGNNNNNNNNNNNNNNNNNNNNNNNNGTCGAGGGTAAACAAATTCCAAAGGGTTTCACCTTCGATGACCACTTTGAAGAANNNNNNNNATTCTTCAGTCGGAAGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAACAGAAGANANATATGNTTNTGCCCTAATTTCTACCAGGTTTNNTGGNCCANTTTGATGGAAGCACTTCGNAAAGGCTNNCAATNGAAGCAANGGNNNNNTTNATTAAGAACTANGGATGGTGNNANNNNNNNTNTTTGTTCGGNAGATNGGANATGAGGTTNNTGTTNGCATTNNNGTANGCNNAGAANTAACCNANTTTCTTCGANNAACTTCTTACNCTNAGAAGCTCNTTCAATTNGGNAACTNCNATTATGTACCCAGCNNCCNCNGCGACGGTNATNANCCCTAGAATAGNTNGGAGAAGGTTGGNGGTTAANTGNGGNAGANCNATTTCAAGAGCNGCCAAGNACNGAACGAGNGCCTNGGGANNAGNANAGGNNAGTCNGNANACCGCGAGGTGAATGAGAAGTCNGGATTAAAGGTGGTTNGATTAAAGGGCCNTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_13806344_13806397_no-0_oy-0_156934_112955_TRUE
SEQUENCE_TARGET=950,153
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGGGGAANAAANCGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14387778_14388156_can-0_hi-0_6858_11074_TRUE
SEQUENCE_TARGET=950,478
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNAAGGCGACGGTGAGATGCGAACGAGTGTGAGAGTCGANNNNNNNNNNNNNNNNGGCGANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGAGNGTTTAGAGAGGAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_11602318_11602767_oy-0_ler-0_690398_179002_TRUE
SEQUENCE_TARGET=950,549
SEQUENCE_TEMPLATE=NCACAATACGACCACCGTGNAAACCTCGACGGAGCGACNGAAATGACAAAGGAGATGATGAGAAACGAAGGAGGTTACGANGAGACNGAGAGGCNAAANNAGGTTTAACGAAAGCNGCTTTAATGGTGGAAACGAANGTGANAGACGTGGCTGAATAAACACGACCAGGTGTTATGATATTGAACGCCATTTGTTTGGCTTTTTATCTNCTCTCTTCCTTCTTCTCTTGACAACAGCTTTTTNTGACTCTCTCTCAATCNTTTCTCAGATTTTTCNTTTTCTCTGGAACACTCNTCTAANTTGGGCCTTACNTTTNGGNCNTTTANCCCAATATACATNCACTCGAAATTGCATTANTCTCTGTTCAAGTTTAGCTATGNCANCCTTGTCGGAATCATTCGTGTACCTGGTTCAAACCCTANNNNTGGTTTNATGAGGCNATGTGATGAGNCTCTGACAAAAGANAANTTNGAAGTTAAAGTCACATGGAAAAAANNAANCAACTAACGGCTGGTGAGAGAAGAAGAGTCGTTCGTATTTAAAACATGAGCATGAATATTTTGACCTCAATAAAGGGACTTTGNNTCANTAACATTCATATTCTTNTTTTTCCAACCTAACGCAATTNTGNAGACCGATCAATCAGACGGTTGTTTCTATACCACACAAGGNAANANNNNTANANTTTGGATCGANATTGGAACCAAAACTAGTAAGTGGGTTTNNTTATGCTTNGATTTNTTTTNNTTTNTGTTNTTTGNNNNTNGANNNNNTCGNTTCNATNNTTTGTTTNTNNNGANNNANNAANNNNCTNNNNCNNNNNNNANNNNNNNNANNNTNTNTCNAAANTTTNCNTNTTTTTNNTNCNTGGAATNTTTGTAGTGTACCCGAAAGTGAAAATTGACNATGANNCTGGTANATACAAGCAGANGGGTTCTTGTCTGAACTCNAACTATCTCCAAACTCGTGTCTCTGGTAATTTCTCTCTNCTTCNNTTTTTNTTTTNCTTTGAGTTTGACTCTCATTTAGATTTTGAGCTAGAANTTTAGGGTTGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTTTAGCTATATGTTTAGGAATTAGGAACATTGACTTGGTTTTTGANAGAGATCNTNNNNNNNNNNNNNATATGGGTTCTTTGTTGTTGCCAATTNAANCGAATGCAAANTTTNAANAGAATCTNTTCTGATGTTCTCTCTTTCTCGTTNGGACAGANAATAANCAAGAAGATGATTTACAAATATACGTGGCTAAGATTCCTAAGATTTACATACCAAGTGTCCTCATNTCTCANAGTGAATCCAAAGAAATGAATAAGCCTATNAGAGGAGCTGACATTGAAGTAAAACCAANAACCAAAGCCAGTCCAANCCTACGTCCACGCGCTNTTGTATCAAGTCCCGGTATGGNNNNTTNTTAGCTGNTTTNCATCTCNAANGTTTTCATTTNCTGGACTGTTTCCATGTGNTTTAGCTATAGTGTCACACAATGTTGAACTCAAATATNTCNAAAACAATGACTTNTGCTTTTGAATTTTCTCTTCTTTTAGATAATGATGCNATGATCGGCAACATGAACATGATTGAAGAAAGGAAAGCTAAGAAGGGTTTGAAGAGTAANGATCATATTAAGAGTAGGGCTTCTCAGCTTAAGAATGTTNATACCAATGTGAAGATTTCTCATCGTATAGTCGCTAAGCTACCGGGAGTCAATTCAAGGNATCACAAATGATCAGCTTCTCTATTAATGATNCCGGTCAGATGGAAGTCATGGAACCACATAGTCACCGTTTTACNTTTTGAAAACGTAATTCCACTNTTTTTTACNTTTCNCAACATTTATATTCACATTTTTGANTAGTNGTANCTTATATATGATGCACATGTNAAAANTATAACTNAAATTAAANANAAGTCATTCATCCATANAATANTTAGTTTTTNTTTGGCATTATTTAGCATAAATTTTGTTCANTTCAAATGTTTCTATCTTATTTTTNNTGAGGATAAGCGANGATGTAGAAGCTGCTCTTTATCACATTAGNAAGGCCATTGAATATCTCAAAGAGATAAACAAATGCGGATCCANTATAGGAAAAGGAACNTATGTGCCTCTTCTTAAGTTCTTAGCTGANATGGGAATGGTAGAGGAATTCCAANTTTCCANGNATGTTATTTTAGAGGCAAANAATGGTTNGNTACNAAGATGGAACCACTCTCATATAACNNTTTTTTTNATTAGGNTTTAAACANAAACATTGGATTTGGATTAATATACTAACTACACATAATNGTTCGATACCAAGNAAAAAANAATANACATTCTTTTTTGNTTTNNTTTAAAGACGTTNAGATAAACAAAGATTGAACTCGTNGGGGACAAGGAAGAACANTGTAACGCGTTATTAAGCAGCTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.992426
PRIMER_LEFT_0_PENALTY=0.923785
PRIMER_RIGHT_0_PENALTY=0.068642
PRIMER_LEFT_0_SEQUENCE=CCGATCAATCAGACGGTTGT
PRIMER_RIGHT_0_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_0=634,20
PRIMER_RIGHT_0=1617,20
PRIMER_LEFT_0_TM=60.924
PRIMER_RIGHT_0_TM=59.931
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=11.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=984
PRIMER_PAIR_1_PENALTY=2.791245
PRIMER_LEFT_1_PENALTY=2.722603
PRIMER_RIGHT_1_PENALTY=0.068642
PRIMER_LEFT_1_SEQUENCE=ACCGATCAATCAGACGGTTGT
PRIMER_RIGHT_1_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_1=633,21
PRIMER_RIGHT_1=1617,20
PRIMER_LEFT_1_TM=61.723
PRIMER_RIGHT_1_TM=59.931
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=985
PRIMER_PAIR_2_PENALTY=3.894535
PRIMER_LEFT_2_PENALTY=3.825893
PRIMER_RIGHT_2_PENALTY=0.068642
PRIMER_LEFT_2_SEQUENCE=GATCAATCAGACGGTTGTTTCT
PRIMER_RIGHT_2_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_2=636,22
PRIMER_RIGHT_2=1617,20
PRIMER_LEFT_2_TM=58.174
PRIMER_RIGHT_2_TM=59.931
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=982
PRIMER_PAIR_3_PENALTY=4.350606
PRIMER_LEFT_3_PENALTY=4.281964
PRIMER_RIGHT_3_PENALTY=0.068642
PRIMER_LEFT_3_SEQUENCE=TCAATCAGACGGTTGTTTCT
PRIMER_RIGHT_3_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_3=638,20
PRIMER_RIGHT_3=1617,20
PRIMER_LEFT_3_TM=55.718
PRIMER_RIGHT_3_TM=59.931
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=980
PRIMER_PAIR_4_PENALTY=4.701899
PRIMER_LEFT_4_PENALTY=4.633257
PRIMER_RIGHT_4_PENALTY=0.068642
PRIMER_LEFT_4_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_4_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_4=877,22
PRIMER_RIGHT_4=1617,20
PRIMER_LEFT_4_TM=57.367
PRIMER_RIGHT_4_TM=59.931
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=741
PRIMER_PAIR_5_PENALTY=4.701899
PRIMER_LEFT_5_PENALTY=4.633257
PRIMER_RIGHT_5_PENALTY=0.068642
PRIMER_LEFT_5_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_5_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_5=876,22
PRIMER_RIGHT_5=1617,20
PRIMER_LEFT_5_TM=57.367
PRIMER_RIGHT_5_TM=59.931
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=11.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=742
PRIMER_PAIR_6_PENALTY=4.701899
PRIMER_LEFT_6_PENALTY=4.633257
PRIMER_RIGHT_6_PENALTY=0.068642
PRIMER_LEFT_6_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_6_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_6=875,22
PRIMER_RIGHT_6=1617,20
PRIMER_LEFT_6_TM=57.367
PRIMER_RIGHT_6_TM=59.931
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=11.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=743
PRIMER_PAIR_7_PENALTY=5.073451
PRIMER_LEFT_7_PENALTY=5.004810
PRIMER_RIGHT_7_PENALTY=0.068642
PRIMER_LEFT_7_SEQUENCE=CAATCAGACGGTTGTTTCTATACC
PRIMER_RIGHT_7_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_7=639,24
PRIMER_RIGHT_7=1617,20
PRIMER_LEFT_7_TM=58.995
PRIMER_RIGHT_7_TM=59.931
PRIMER_LEFT_7_GC_PERCENT=41.667
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.8000
PRIMER_RIGHT_7_END_STABILITY=11.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=979
PRIMER_PAIR_8_PENALTY=5.220446
PRIMER_LEFT_8_PENALTY=5.151805
PRIMER_RIGHT_8_PENALTY=0.068642
PRIMER_LEFT_8_SEQUENCE=TGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_8_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_8=877,21
PRIMER_RIGHT_8=1617,20
PRIMER_LEFT_8_TM=55.848
PRIMER_RIGHT_8_TM=59.931
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=11.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=741
PRIMER_PAIR_9_PENALTY=5.220446
PRIMER_LEFT_9_PENALTY=5.151805
PRIMER_RIGHT_9_PENALTY=0.068642
PRIMER_LEFT_9_SEQUENCE=TTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_9_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_9=876,21
PRIMER_RIGHT_9=1617,20
PRIMER_LEFT_9_TM=55.848
PRIMER_RIGHT_9_TM=59.931
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=11.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=742
PRIMER_PAIR_10_PENALTY=5.550361
PRIMER_LEFT_10_PENALTY=5.481719
PRIMER_RIGHT_10_PENALTY=0.068642
PRIMER_LEFT_10_SEQUENCE=GACCGATCAATCAGACGGTTGT
PRIMER_RIGHT_10_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_10=632,22
PRIMER_RIGHT_10=1617,20
PRIMER_LEFT_10_TM=63.482
PRIMER_RIGHT_10_TM=59.931
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=11.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=986
PRIMER_PAIR_11_PENALTY=5.634205
PRIMER_LEFT_11_PENALTY=5.565563
PRIMER_RIGHT_11_PENALTY=0.068642
PRIMER_LEFT_11_SEQUENCE=TTTGTAGTGTACCCGAAAGTG
PRIMER_RIGHT_11_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_11=875,21
PRIMER_RIGHT_11=1617,20
PRIMER_LEFT_11_TM=55.434
PRIMER_RIGHT_11_TM=59.931
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=743
PRIMER_PAIR_12_PENALTY=5.661987
PRIMER_LEFT_12_PENALTY=5.593345
PRIMER_RIGHT_12_PENALTY=0.068642
PRIMER_LEFT_12_SEQUENCE=CGATCAATCAGACGGTTGTTTCT
PRIMER_RIGHT_12_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_12=635,23
PRIMER_RIGHT_12=1617,20
PRIMER_LEFT_12_TM=62.593
PRIMER_RIGHT_12_TM=59.931
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=11.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=983
PRIMER_PAIR_13_PENALTY=5.683448
PRIMER_LEFT_13_PENALTY=5.614806
PRIMER_RIGHT_13_PENALTY=0.068642
PRIMER_LEFT_13_SEQUENCE=TCAATCAGACGGTTGTTTCTATACC
PRIMER_RIGHT_13_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_13=638,25
PRIMER_RIGHT_13=1617,20
PRIMER_LEFT_13_TM=60.615
PRIMER_RIGHT_13_TM=59.931
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.8000
PRIMER_RIGHT_13_END_STABILITY=11.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=980
PRIMER_PAIR_14_PENALTY=5.714846
PRIMER_LEFT_14_PENALTY=4.633257
PRIMER_RIGHT_14_PENALTY=1.081589
PRIMER_LEFT_14_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_14_SEQUENCE=TTCCATGACTTCCATCTGACC
PRIMER_LEFT_14=877,22
PRIMER_RIGHT_14=1808,21
PRIMER_LEFT_14_TM=57.367
PRIMER_RIGHT_14_TM=59.918
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=932
PRIMER_PAIR_15_PENALTY=5.714846
PRIMER_LEFT_15_PENALTY=4.633257
PRIMER_RIGHT_15_PENALTY=1.081589
PRIMER_LEFT_15_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_15_SEQUENCE=TTCCATGACTTCCATCTGACC
PRIMER_LEFT_15=876,22
PRIMER_RIGHT_15=1808,21
PRIMER_LEFT_15_TM=57.367
PRIMER_RIGHT_15_TM=59.918
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=933
PRIMER_PAIR_16_PENALTY=5.714846
PRIMER_LEFT_16_PENALTY=4.633257
PRIMER_RIGHT_16_PENALTY=1.081589
PRIMER_LEFT_16_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_16_SEQUENCE=TTCCATGACTTCCATCTGACC
PRIMER_LEFT_16=875,22
PRIMER_RIGHT_16=1808,21
PRIMER_LEFT_16_TM=57.367
PRIMER_RIGHT_16_TM=59.918
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=934
PRIMER_PAIR_17_PENALTY=5.858824
PRIMER_LEFT_17_PENALTY=4.633257
PRIMER_RIGHT_17_PENALTY=1.225567
PRIMER_LEFT_17_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_17_SEQUENCE=ATGTGGTTCCATGACTTCCA
PRIMER_LEFT_17=877,22
PRIMER_RIGHT_17=1814,20
PRIMER_LEFT_17_TM=57.367
PRIMER_RIGHT_17_TM=58.774
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=938
PRIMER_PAIR_18_PENALTY=5.858824
PRIMER_LEFT_18_PENALTY=4.633257
PRIMER_RIGHT_18_PENALTY=1.225567
PRIMER_LEFT_18_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_18_SEQUENCE=ATGTGGTTCCATGACTTCCA
PRIMER_LEFT_18=876,22
PRIMER_RIGHT_18=1814,20
PRIMER_LEFT_18_TM=57.367
PRIMER_RIGHT_18_TM=58.774
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=939
PRIMER_PAIR_19_PENALTY=5.858824
PRIMER_LEFT_19_PENALTY=4.633257
PRIMER_RIGHT_19_PENALTY=1.225567
PRIMER_LEFT_19_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_19_SEQUENCE=ATGTGGTTCCATGACTTCCA
PRIMER_LEFT_19=875,22
PRIMER_RIGHT_19=1814,20
PRIMER_LEFT_19_TM=57.367
PRIMER_RIGHT_19_TM=58.774
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=940
PRIMER_PAIR_20_PENALTY=6.017045
PRIMER_LEFT_20_PENALTY=5.948404
PRIMER_RIGHT_20_PENALTY=0.068642
PRIMER_LEFT_20_SEQUENCE=GTAGTGTACCCGAAAGTGAAAA
PRIMER_RIGHT_20_SEQUENCE=AATCATGTTCATGTTGCCGA
PRIMER_LEFT_20=878,22
PRIMER_RIGHT_20=1617,20
PRIMER_LEFT_20_TM=56.052
PRIMER_RIGHT_20_TM=59.931
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.3000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=740
PRIMER_PAIR_21_PENALTY=6.207838
PRIMER_LEFT_21_PENALTY=4.633257
PRIMER_RIGHT_21_PENALTY=1.574581
PRIMER_LEFT_21_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_21_SEQUENCE=TCCATGACTTCCATCTGACC
PRIMER_LEFT_21=877,22
PRIMER_RIGHT_21=1807,20
PRIMER_LEFT_21_TM=57.367
PRIMER_RIGHT_21_TM=58.425
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=931
PRIMER_PAIR_22_PENALTY=6.207838
PRIMER_LEFT_22_PENALTY=4.633257
PRIMER_RIGHT_22_PENALTY=1.574581
PRIMER_LEFT_22_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_22_SEQUENCE=TCCATGACTTCCATCTGACC
PRIMER_LEFT_22=876,22
PRIMER_RIGHT_22=1807,20
PRIMER_LEFT_22_TM=57.367
PRIMER_RIGHT_22_TM=58.425
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=932
PRIMER_PAIR_23_PENALTY=6.207838
PRIMER_LEFT_23_PENALTY=4.633257
PRIMER_RIGHT_23_PENALTY=1.574581
PRIMER_LEFT_23_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_23_SEQUENCE=TCCATGACTTCCATCTGACC
PRIMER_LEFT_23=875,22
PRIMER_RIGHT_23=1807,20
PRIMER_LEFT_23_TM=57.367
PRIMER_RIGHT_23_TM=58.425
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=933
PRIMER_PAIR_24_PENALTY=6.233393
PRIMER_LEFT_24_PENALTY=5.151805
PRIMER_RIGHT_24_PENALTY=1.081589
PRIMER_LEFT_24_SEQUENCE=TGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_24_SEQUENCE=TTCCATGACTTCCATCTGACC
PRIMER_LEFT_24=877,21
PRIMER_RIGHT_24=1808,21
PRIMER_LEFT_24_TM=55.848
PRIMER_RIGHT_24_TM=59.918
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=932
PRIMER_PAIR_25_PENALTY=6.233393
PRIMER_LEFT_25_PENALTY=5.151805
PRIMER_RIGHT_25_PENALTY=1.081589
PRIMER_LEFT_25_SEQUENCE=TTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_25_SEQUENCE=TTCCATGACTTCCATCTGACC
PRIMER_LEFT_25=876,21
PRIMER_RIGHT_25=1808,21
PRIMER_LEFT_25_TM=55.848
PRIMER_RIGHT_25_TM=59.918
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=933
PRIMER_PAIR_26_PENALTY=6.377371
PRIMER_LEFT_26_PENALTY=5.151805
PRIMER_RIGHT_26_PENALTY=1.225567
PRIMER_LEFT_26_SEQUENCE=TGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_26_SEQUENCE=ATGTGGTTCCATGACTTCCA
PRIMER_LEFT_26=877,21
PRIMER_RIGHT_26=1814,20
PRIMER_LEFT_26_TM=55.848
PRIMER_RIGHT_26_TM=58.774
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=938
PRIMER_PAIR_27_PENALTY=6.377371
PRIMER_LEFT_27_PENALTY=5.151805
PRIMER_RIGHT_27_PENALTY=1.225567
PRIMER_LEFT_27_SEQUENCE=TTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_27_SEQUENCE=ATGTGGTTCCATGACTTCCA
PRIMER_LEFT_27=876,21
PRIMER_RIGHT_27=1814,20
PRIMER_LEFT_27_TM=55.848
PRIMER_RIGHT_27_TM=58.774
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=939
PRIMER_PAIR_28_PENALTY=6.407551
PRIMER_LEFT_28_PENALTY=4.633257
PRIMER_RIGHT_28_PENALTY=1.774294
PRIMER_LEFT_28_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_28_SEQUENCE=TGTGGTTCCATGACTTCCATC
PRIMER_LEFT_28=877,22
PRIMER_RIGHT_28=1813,21
PRIMER_LEFT_28_TM=57.367
PRIMER_RIGHT_28_TM=60.774
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=937
PRIMER_PAIR_29_PENALTY=6.407551
PRIMER_LEFT_29_PENALTY=4.633257
PRIMER_RIGHT_29_PENALTY=1.774294
PRIMER_LEFT_29_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_29_SEQUENCE=TGTGGTTCCATGACTTCCATC
PRIMER_LEFT_29=876,22
PRIMER_RIGHT_29=1813,21
PRIMER_LEFT_29_TM=57.367
PRIMER_RIGHT_29_TM=60.774
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=8.1000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=938
PRIMER_PAIR_30_PENALTY=6.407551
PRIMER_LEFT_30_PENALTY=4.633257
PRIMER_RIGHT_30_PENALTY=1.774294
PRIMER_LEFT_30_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_30_SEQUENCE=TGTGGTTCCATGACTTCCATC
PRIMER_LEFT_30=875,22
PRIMER_RIGHT_30=1813,21
PRIMER_LEFT_30_TM=57.367
PRIMER_RIGHT_30_TM=60.774
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=939
PRIMER_PAIR_31_PENALTY=6.647152
PRIMER_LEFT_31_PENALTY=5.565563
PRIMER_RIGHT_31_PENALTY=1.081589
PRIMER_LEFT_31_SEQUENCE=TTTGTAGTGTACCCGAAAGTG
PRIMER_RIGHT_31_SEQUENCE=TTCCATGACTTCCATCTGACC
PRIMER_LEFT_31=875,21
PRIMER_RIGHT_31=1808,21
PRIMER_LEFT_31_TM=55.434
PRIMER_RIGHT_31_TM=59.918
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=934
PRIMER_PAIR_32_PENALTY=6.723003
PRIMER_LEFT_32_PENALTY=4.633257
PRIMER_RIGHT_32_PENALTY=2.089746
PRIMER_LEFT_32_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_32_SEQUENCE=TGGTTCCATGACTTCCATCT
PRIMER_LEFT_32=877,22
PRIMER_RIGHT_32=1811,20
PRIMER_LEFT_32_TM=57.367
PRIMER_RIGHT_32_TM=57.910
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=6.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=935
PRIMER_PAIR_33_PENALTY=6.723003
PRIMER_LEFT_33_PENALTY=4.633257
PRIMER_RIGHT_33_PENALTY=2.089746
PRIMER_LEFT_33_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_33_SEQUENCE=TGGTTCCATGACTTCCATCT
PRIMER_LEFT_33=876,22
PRIMER_RIGHT_33=1811,20
PRIMER_LEFT_33_TM=57.367
PRIMER_RIGHT_33_TM=57.910
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=936
PRIMER_PAIR_34_PENALTY=6.723003
PRIMER_LEFT_34_PENALTY=4.633257
PRIMER_RIGHT_34_PENALTY=2.089746
PRIMER_LEFT_34_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_34_SEQUENCE=TGGTTCCATGACTTCCATCT
PRIMER_LEFT_34=875,22
PRIMER_RIGHT_34=1811,20
PRIMER_LEFT_34_TM=57.367
PRIMER_RIGHT_34_TM=57.910
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=937
PRIMER_PAIR_35_PENALTY=6.726386
PRIMER_LEFT_35_PENALTY=5.151805
PRIMER_RIGHT_35_PENALTY=1.574581
PRIMER_LEFT_35_SEQUENCE=TGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_35_SEQUENCE=TCCATGACTTCCATCTGACC
PRIMER_LEFT_35=877,21
PRIMER_RIGHT_35=1807,20
PRIMER_LEFT_35_TM=55.848
PRIMER_RIGHT_35_TM=58.425
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=931
PRIMER_PAIR_36_PENALTY=6.726386
PRIMER_LEFT_36_PENALTY=5.151805
PRIMER_RIGHT_36_PENALTY=1.574581
PRIMER_LEFT_36_SEQUENCE=TTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_36_SEQUENCE=TCCATGACTTCCATCTGACC
PRIMER_LEFT_36=876,21
PRIMER_RIGHT_36=1807,20
PRIMER_LEFT_36_TM=55.848
PRIMER_RIGHT_36_TM=58.425
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=932
PRIMER_PAIR_37_PENALTY=6.776674
PRIMER_LEFT_37_PENALTY=4.633257
PRIMER_RIGHT_37_PENALTY=2.143417
PRIMER_LEFT_37_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_37_SEQUENCE=GTGGTTCCATGACTTCCATCT
PRIMER_LEFT_37=877,22
PRIMER_RIGHT_37=1812,21
PRIMER_LEFT_37_TM=57.367
PRIMER_RIGHT_37_TM=58.857
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.3000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=936
PRIMER_PAIR_38_PENALTY=6.776674
PRIMER_LEFT_38_PENALTY=4.633257
PRIMER_RIGHT_38_PENALTY=2.143417
PRIMER_LEFT_38_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_38_SEQUENCE=GTGGTTCCATGACTTCCATCT
PRIMER_LEFT_38=876,22
PRIMER_RIGHT_38=1812,21
PRIMER_LEFT_38_TM=57.367
PRIMER_RIGHT_38_TM=58.857
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=937
PRIMER_PAIR_39_PENALTY=6.776674
PRIMER_LEFT_39_PENALTY=4.633257
PRIMER_RIGHT_39_PENALTY=2.143417
PRIMER_LEFT_39_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_39_SEQUENCE=GTGGTTCCATGACTTCCATCT
PRIMER_LEFT_39=875,22
PRIMER_RIGHT_39=1812,21
PRIMER_LEFT_39_TM=57.367
PRIMER_RIGHT_39_TM=58.857
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=938
PRIMER_PAIR_40_PENALTY=6.780552
PRIMER_LEFT_40_PENALTY=4.633257
PRIMER_RIGHT_40_PENALTY=2.147295
PRIMER_LEFT_40_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_40_SEQUENCE=CTATGTGGTTCCATGACTTCCA
PRIMER_LEFT_40=877,22
PRIMER_RIGHT_40=1816,22
PRIMER_LEFT_40_TM=57.367
PRIMER_RIGHT_40_TM=59.853
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.3000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=940
PRIMER_PAIR_41_PENALTY=6.780552
PRIMER_LEFT_41_PENALTY=4.633257
PRIMER_RIGHT_41_PENALTY=2.147295
PRIMER_LEFT_41_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_41_SEQUENCE=CTATGTGGTTCCATGACTTCCA
PRIMER_LEFT_41=876,22
PRIMER_RIGHT_41=1816,22
PRIMER_LEFT_41_TM=57.367
PRIMER_RIGHT_41_TM=59.853
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=941
PRIMER_PAIR_42_PENALTY=6.780552
PRIMER_LEFT_42_PENALTY=4.633257
PRIMER_RIGHT_42_PENALTY=2.147295
PRIMER_LEFT_42_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_42_SEQUENCE=CTATGTGGTTCCATGACTTCCA
PRIMER_LEFT_42=875,22
PRIMER_RIGHT_42=1816,22
PRIMER_LEFT_42_TM=57.367
PRIMER_RIGHT_42_TM=59.853
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=942
PRIMER_PAIR_43_PENALTY=6.791130
PRIMER_LEFT_43_PENALTY=5.565563
PRIMER_RIGHT_43_PENALTY=1.225567
PRIMER_LEFT_43_SEQUENCE=TTTGTAGTGTACCCGAAAGTG
PRIMER_RIGHT_43_SEQUENCE=ATGTGGTTCCATGACTTCCA
PRIMER_LEFT_43=875,21
PRIMER_RIGHT_43=1814,20
PRIMER_LEFT_43_TM=55.434
PRIMER_RIGHT_43_TM=58.774
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=940
PRIMER_PAIR_44_PENALTY=6.797729
PRIMER_LEFT_44_PENALTY=4.633257
PRIMER_RIGHT_44_PENALTY=2.164472
PRIMER_LEFT_44_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_44_SEQUENCE=TATGTGGTTCCATGACTTCCA
PRIMER_LEFT_44=877,22
PRIMER_RIGHT_44=1815,21
PRIMER_LEFT_44_TM=57.367
PRIMER_RIGHT_44_TM=58.836
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=939
PRIMER_PAIR_45_PENALTY=6.797729
PRIMER_LEFT_45_PENALTY=4.633257
PRIMER_RIGHT_45_PENALTY=2.164472
PRIMER_LEFT_45_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_45_SEQUENCE=TATGTGGTTCCATGACTTCCA
PRIMER_LEFT_45=876,22
PRIMER_RIGHT_45=1815,21
PRIMER_LEFT_45_TM=57.367
PRIMER_RIGHT_45_TM=58.836
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=940
PRIMER_PAIR_46_PENALTY=6.797729
PRIMER_LEFT_46_PENALTY=4.633257
PRIMER_RIGHT_46_PENALTY=2.164472
PRIMER_LEFT_46_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_46_SEQUENCE=TATGTGGTTCCATGACTTCCA
PRIMER_LEFT_46=875,22
PRIMER_RIGHT_46=1815,21
PRIMER_LEFT_46_TM=57.367
PRIMER_RIGHT_46_TM=58.836
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=941
PRIMER_PAIR_47_PENALTY=6.886900
PRIMER_LEFT_47_PENALTY=4.633257
PRIMER_RIGHT_47_PENALTY=2.253643
PRIMER_LEFT_47_SEQUENCE=TGTAGTGTACCCGAAAGTGAAA
PRIMER_RIGHT_47_SEQUENCE=GTGGTTCCATGACTTCCATC
PRIMER_LEFT_47=877,22
PRIMER_RIGHT_47=1812,20
PRIMER_LEFT_47_TM=57.367
PRIMER_RIGHT_47_TM=57.746
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.3000
PRIMER_RIGHT_47_END_STABILITY=8.1000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=936
PRIMER_PAIR_48_PENALTY=6.886900
PRIMER_LEFT_48_PENALTY=4.633257
PRIMER_RIGHT_48_PENALTY=2.253643
PRIMER_LEFT_48_SEQUENCE=TTGTAGTGTACCCGAAAGTGAA
PRIMER_RIGHT_48_SEQUENCE=GTGGTTCCATGACTTCCATC
PRIMER_LEFT_48=876,22
PRIMER_RIGHT_48=1812,20
PRIMER_LEFT_48_TM=57.367
PRIMER_RIGHT_48_TM=57.746
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=8.1000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=937
PRIMER_PAIR_49_PENALTY=6.886900
PRIMER_LEFT_49_PENALTY=4.633257
PRIMER_RIGHT_49_PENALTY=2.253643
PRIMER_LEFT_49_SEQUENCE=TTTGTAGTGTACCCGAAAGTGA
PRIMER_RIGHT_49_SEQUENCE=GTGGTTCCATGACTTCCATC
PRIMER_LEFT_49=875,22
PRIMER_RIGHT_49=1812,20
PRIMER_LEFT_49_TM=57.367
PRIMER_RIGHT_49_TM=57.746
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=8.1000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=938
=
SEQUENCE_ID=MAGIC.433_3_14538534_14538802_sf-2_oy-0_30542_760040_TRUE
SEQUENCE_TARGET=950,368
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATATAATGGAAGTTCGATTAAGNNTNTCGCATACNNGAGGTGGTTGGCTCGGGTNCNNNNNNNNNNNNNNAAAGTTGNATTTAGTATANTTTGTTNNNNNNNNNNNNNNNNNNTACCANGTTATTCNGGATAACTNGATTTCTAANCACNCAGNNNNNNNNNNATAAGAAACTNNNNNNNNNNNNNNNNNNNNNNNGACACCAGTGTTTATTTCAANNNNNNNNNNNNNNNNNNNNNCNNNNGNTTAGCACCAATGTTTATGATAACTGNGTTTTAGTTTCCNANGNGNTNGNCNCNNTACNAGTTAGGANNNNGNTTTTGTAGGAGTGAATGTATTNGAGCNTTTTCGGGCGAAGCTTTGTCTTTCATGCNCGAGGTNGTTAGCTNNGTACCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGGAGCTNTTTTTTNACTNNNNNGAAGNNTNCNTTTNNTNGGTAGANGAGNCTTTTTGCTCGGTAGACGAGTCNNTTTNCTCGGTAGACNAGTGTTTTTGCNAGTTTATGNAAAANATANAANAGAGANTGCATATATATGATTGAGNTGTGCTTGTTANATTATAATNNATAACANTTATCTTAGNGGTTTGGANNTAGAGATGATGACATCTACGACATGGACGATCTGAATCNNTNAGATTNTAAANGTNGTTGTATGGAANNCTANATTTGAGATGTTNGTAGTNNGGAGGTCNCNTCTTTGCGNTAGAANNAGAGANGCAANGATAAANGCNNNNNNNNNNNNNGAGTATGTCAANCAAGCTTAATTAATATGTNAGAANGTAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANGCGNTAGAGNGAGGGGTCCATGAACGATTNTAAGTNATATTCCATAGTNGCTTGNTGGTGTACTNNTTTTTGCTGATTTGGAGAATAGCTGATAACTTTTGNTTNAATNNTTAGGCTTTNNATCNCACCGTTNAGTCTTTANATGGTAGTTCCATGAANGTCTCCTCCANATTTNAGTCANATCNGATATTGCATGAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTNCCTTTGTTTTGCTGATTCAGAGANGCNNNGNTAACTTTAGTCTGGACNGTTGANNNNTNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_13151972_13152074_tsu-0_hi-0_2352_9264_TRUE
SEQUENCE_TARGET=950,202
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAGAGTTTCGGCTANTGAAGCGTTGNTAGCNTCTTCTCCAGACNTTGCTNCTCACTTCGGAAATGTANTANATAGAANAAATNTNANNACTCAAGAATCCTNCGTCCCCACAGANGNCGCCAAAATNTGGATGTCGGATTCACACACTCAANTATTTATTTNTTTANTGAGAAGGATTATTCTTGAGGACAAATGTACTAGAAAATANANNAATGATTATAGAGAAAACTATGAGTTTTGTAAGAAAATNNNNNANAATCCANTATATAAAANCANATGTTCAAAAACAAATATGCCTAAGAAAAGGGAAATTTGGTCGTTTGTACCACATTCACNAAAAAAANCTANCCGTTTGTANCACATTCATGGAGACATNCAAGTTTNNTGGACCAATATGCCCTTGACNNAGACATGTGTCAGAGNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_1539793_1539806_po-0_rsch-4_79772_23981_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=CCTGTCCAGCTTCAACAGCTAAATACAAGGGAGAAANCCCATCGTTGTTTGNTACAAAAGACAAACTTTGCTCTGCANANACCAAACAGGAAGCCACNTTCANATGTTTTCNTTTTAANGNNACATGTAAAGCATTGTCTTGGTGNNTGTCCTTGGCAAAGTATATCTTTTTGGCAACACCAGGCTTATTACAAGATATATCTTTGATAAAACTAACCAGAGCCTCAACNACAGCGAGATGGCCTGCACCTGCTGCAACATGGAGAGCAACTTCCCCCATGGAATTTGACTTCATAAGCAGACCTGGGTACGCATTGAGTATATAACACACTAAGTCGGTGTGACCCGCGGCTGCAGCAAGATGAAGCATTGTATTNCCATGATCGTTCACTGAGCTTAAAGCAATGTTAACATCAANTTTCATATCTCTCANGTAAGTTTCATCACCTGCTCTTACAGCGGCGATGGTCTTTGGACCCATTGGCACAGTTTCACTTGTGTCATTGAAGAGANTTGACAAATCGAGAGAGTGCGTACGCCCGGTTTCGTCTGCATAAAACATCCNAGAGCTTGCGTCTTTCTTCNGACGGTCAAANTCCAGATCAGACNTTCCCATATTGATAATTTGGCNNCNAAACAATAAGGAANAANNNNNNNNNNAANANCTACATACGATGAATTGATGAAGTAGAAACAAATATGCTTNNATCATTGNGACTCAAAGNTTTTATATTTAATGATTGAACCGCAAGGTAATTGTATATCATGCACTAACATAGTTGCCTGAANCTTCANANAACAGAANCTCAAAGAGNAAGAAAACAATGAAAACAGAGTAAAAAGTAAGCTTGAGGTTTCTCACCTTATTCGTCAGCCGAAGCAACGACTTGATGCTGGAGGGCTCAGGGAAGGCTTTNCCCAACTTCAATATTTCCAAATGCTTGCAGAACCAACTCCACAATGCTTGAGATATAGATTGGTAAGTGGTATCTACAGNAAANAGAAGTCTTNAGNTTTTANNANTGTCCATCANGGAAGAAGAAATNGTCANNNNNNTAAAAGGAANCANACGCGNACCTTCTTTNTTTTTNCTTTCNCATCAGANNAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTATANTNTNTAANGGNTTTNTCAAGTCTACCTTATTTGTNNTATTACANAGAAAACAAGNCATGNTCCAAAAGAAGAAAGCANTNNGTGGGATACTNNNNNNNNNNNNTATATAGAAANNCAACGACTTNTGGGTTTNTTCAACTTCTAANGTCAACTATCTNNNNNNNNNTCAACATNTCTCNAAGCTACTATCCAAAAATTCCATTACTCTNGAAAGTCTTGAAGAAAANCGGACGCAATAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTCAACGAATTAGAAAAACAANCGGCGACAAGACCGATCTNTTGTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTNAGAGTAGTGCNCCGTCCTCAATNCTCCACNTCTGCNTNNCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCCGTCTCCGNCNATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCNNNNAAGGTNTGTAATATTNGGCCTATAANAGNTTACANATGGCCCNNNNNNNNNNNNNNNTTNNTNGTNTNTACAATGAAAAGTCGAACTCAACGTNGTGTTCACANTTGGGCCTATAAAGNTCAACAAAANNTNGGCNCAGTTTAATATAGNCTTTTCATCTCCGCGACGCNGTTTCGTAAGAGTGNNNANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=2
PRIMER_RIGHT_NUM_RETURNED=2
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=2
PRIMER_PAIR_0_PENALTY=5.829829
PRIMER_LEFT_0_PENALTY=1.795587
PRIMER_RIGHT_0_PENALTY=4.034241
PRIMER_LEFT_0_SEQUENCE=CAACGACTTGATGCTGGAGG
PRIMER_RIGHT_0_SEQUENCE=ACGTTGAGTTCGACTTTTCA
PRIMER_LEFT_0=880,20
PRIMER_RIGHT_0=1874,20
PRIMER_LEFT_0_TM=61.796
PRIMER_RIGHT_0_TM=55.966
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=995
PRIMER_PAIR_1_PENALTY=6.564332
PRIMER_LEFT_1_PENALTY=2.530091
PRIMER_RIGHT_1_PENALTY=4.034241
PRIMER_LEFT_1_SEQUENCE=AACGACTTGATGCTGGAGGG
PRIMER_RIGHT_1_SEQUENCE=ACGTTGAGTTCGACTTTTCA
PRIMER_LEFT_1=881,20
PRIMER_RIGHT_1=1874,20
PRIMER_LEFT_1_TM=62.530
PRIMER_RIGHT_1_TM=55.966
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=994
=
SEQUENCE_ID=MAGIC.338_5_12247131_12247265_rsch-4_oy-0_26996_498051_TRUE
SEQUENCE_TARGET=950,234
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13268576_13268773_po-0_oy-0_55890_347713_TRUE
SEQUENCE_TARGET=950,297
SEQUENCE_TEMPLATE=TTCTATGTAACAACTTGGCAGCTGTAAACCTGAATGGATGTATTAGCTCAAGCTCTTCTATATGAAAAGACGTTCTTGATGATCAACTTCATCTATGGTTACCTTGGAAAAAACCTCAACAGTAAACATATATTTGTGCTTCNAAAATGGATNTTTAACTTNTGGNAAAAAAAGTTTGATGAATATGTCATAAANTTAAATGTGCCAATGTCGTTTTGTTGCTTGACTTCTACACAGNCAAGNNGAATANCCGACCTTAGCAATTANAGGTTTAAAGTTGTATATCACAAGCTAGNNTTTTATTTTCTTGGGGACAAAANAGTTCCCCAATATGGTATTCNATTACAATGTTAAAGAAGTATGAGATGATTGTAGGGACNTTCTCTTAAAAGTNNNNNNNNTTGTTATATTTGAAAGAGGNACTGATCCAANNAACANATGATGATCTTTTTNNAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTACTCGNTAGGACACTCTACCAAAANAACATAAGGGNNNNNTTTNTAAAACNCTNTTGNTGTTGTTTTTCTTAAGTTTNTGCAACATATCNTACGTAGGNAAAATATCATAAANTTCTTGAACATTGATGCTGATANTGGTAGACCTTCAATGAATTTGAAGATGANCTTNNCTNAAAANAANNTNTAAGANGATTTTCTTTGNCNANGTTCTAATNANNGNGACAAGATCGNTTACTTCACCNAACTNATAATCTTAACAAATAANGTTNTCTACAATAAGTNTCTTCTTGAAGTGTAGTGATTGNTGGACTTCCTAGTTTTAAAACCNACATCAAANCAAAGGTCAACATNTGAGATTAATAATCTCGAAAGGAAAGAGTTTCATAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_109971_110040_oy-0_po-0_100157_871283_TRUE
SEQUENCE_TARGET=950,169
SEQUENCE_TEMPLATE=TGTCANATGATCCAGTTTTCAAACTACATAGGGGGTTTATAATTTCTTTTNTGGACTTAACTATTTACTAATATGTATCCATTACACACATATCCAGAGCCAGCCCATATCTAAAGCCCAGCAAATAGAATATATAACTTTTTAGAATACAACAAATTTGTGTAAAAATAGGAAAGAATCCAAAAATGGAACTTATTGAGGAAGAAAGTGGAAACTACTGTTTGTNGGTGCCAAATAGAAGATGNNNNAAGTAAGTACATAATGAGGTCGAAAAGTCACAAGTCTAAAGTCTTTGTCTGTTTTTTATTTTATTTTGGAGAGTGCATGGCAACTTGCTCCNACGTGAGAGATGCTTGAGGANTCGGAACCTTTCTTTGTTTATTGTAAAACGAAATTTCAGAAAGAAAAAAAAAGATGATGATGACTTGGACGTTCAAATTATCNTGCTTCTATTGACTGACAATTATGGTTGAACTAGAATAAGGGTTTATGGAGCACGCAATTAGACCTTTTCTCATATTCTTNGCATCTTTTACCAACCAATAATAATCCTACTTAAAAACTGGAATCCAGATATGTGTNNNNNNNNNAGAAAAAAGGTNGACTACTATAATTGTGTATGGGTGGAAATGGANNNNTATANCCTGAGTAGGGGTTAGTNGGTCCANTTTGGTNGTGGNNTTAAAANTTGGAAANNTCTTTTTTCNTCACAANTACACTTTTATGTGTGNTTTTTTTTTTTTGTTAATGATNTGTGATTTATTTGCCTACGCTTTTGCACNCATCAACANNCCAATTTTAATTTGATAGGGCNAAATCATGTNTTCTTNANTNANAAAATAGNATAACGGCTTTAATTTNTNANGTTTTGGTTTTNNATNNNAANCACTTAACACNTTAAAACAGTATAAANAGTTTNANATTTACCAATCACAAAATTNTTANCAAAAGNGTTATAAAANNTTTTATTNTNAGNTTTTNTNNANANTTTTTTATACAGNTNTTTCTAACAGTTTTNCACTATTNNNATAAAANTAATAGNTANAAAACTTGNACANCTTTTAGNTACNAANNANNTTNCTTTNATATNTTNTATNNATATANATATATANTTTTAANGTTAAAANGTAAAAGAANAAAAAAAANAANNNNAATAGAGAGAAAGAGAGAATGTNAATAATCAATATACATCTTGAATGTTTTGACGACATCACNGTCTCCNAAAAAAGTTACCAANAACCTTAAAGTAAAAACGAATTAATATTAATTAAATTTTAAAATGATTTAAATATTTTATTGCTGANNTGTTGTTTGNTTGCTTACTNTNNNNNNTTTCCTTGTTTCACTTTTGCATTTCTTGAAACATCATTTAGTTATCTCACTCGGTAATGCACAAACATCATCATTACGTCTCTCTATTTGCTAATTTGCATATTGATCGTAAGAAAAAGAGAGAAGATATACAAACTCAAAATTAATGTTTAATACCTAATTCCAGATATACACATTAACACGTCAATTCTGTAGAATGGTCTTTCGTACAAAAGANATTCTGTTTATACATTACACAAACTTTACGCCTGTCTTTCCAACAGCTAGATCTTCGAATTTCATTCCCAACCATAAAATGTAGTCAAGTCACCTCTTCTTATAACTTAGGTTTCATAGAGTTCTTATTATAAGGGNTTTTCTTGAAATCTCCTTTTAATCGTGTTTCTGTATAATATGAGATCGACAAACATAAANCAAATCACGATCAAACGAGAAGGTTGATTATATTCATCCTAATGGTCGAACANACCCCTTCGTACTCACCAAAGTTAAATNACGTTCTCGGTTCTCATANGAACAATCCTTCTCTAACAACATTACCTGNTGATGTATCCAAGATTATGTATGTCTCGTACATGTTTTATAGTTTACTANTTATTGATCAAACAAAANCATGTTGCGGAACAATGTCAACTTTTGGANTTTCCAANCTCCCATCTTTGTCNAAAACAACCTCTTTTGGATGCATCTTATCGTGTTCCTNTGNATGNAATTAANGAAAACGTTAGNTTTTGAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.059321
PRIMER_LEFT_0_PENALTY=0.376636
PRIMER_RIGHT_0_PENALTY=0.682685
PRIMER_LEFT_0_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_0_SEQUENCE=TGTTGGAAAGACAGGCGTAA
PRIMER_LEFT_0=614,20
PRIMER_RIGHT_0=1593,20
PRIMER_LEFT_0_TM=59.623
PRIMER_RIGHT_0_TM=59.317
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=7.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=980
PRIMER_PAIR_1_PENALTY=1.503657
PRIMER_LEFT_1_PENALTY=0.103187
PRIMER_RIGHT_1_PENALTY=1.400471
PRIMER_LEFT_1_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_1_SEQUENCE=TGATGTTTGTGCATTACCGAG
PRIMER_LEFT_1=489,20
PRIMER_RIGHT_1=1401,21
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=59.600
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=913
PRIMER_PAIR_2_PENALTY=1.556165
PRIMER_LEFT_2_PENALTY=0.103187
PRIMER_RIGHT_2_PENALTY=1.452979
PRIMER_LEFT_2_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_2_SEQUENCE=TGATGTTTGTGCATTACCGA
PRIMER_LEFT_2=489,20
PRIMER_RIGHT_2=1401,20
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=58.547
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=913
PRIMER_PAIR_3_PENALTY=1.585301
PRIMER_LEFT_3_PENALTY=0.902616
PRIMER_RIGHT_3_PENALTY=0.682685
PRIMER_LEFT_3_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_3_SEQUENCE=TGTTGGAAAGACAGGCGTAA
PRIMER_LEFT_3=613,20
PRIMER_RIGHT_3=1593,20
PRIMER_LEFT_3_TM=59.097
PRIMER_RIGHT_3_TM=59.317
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=981
PRIMER_PAIR_4_PENALTY=1.666919
PRIMER_LEFT_4_PENALTY=0.376636
PRIMER_RIGHT_4_PENALTY=1.290283
PRIMER_LEFT_4_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_4_SEQUENCE=CTGTTGGAAAGACAGGCGTAA
PRIMER_LEFT_4=614,20
PRIMER_RIGHT_4=1594,21
PRIMER_LEFT_4_TM=59.623
PRIMER_RIGHT_4_TM=60.290
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=981
PRIMER_PAIR_5_PENALTY=1.771288
PRIMER_LEFT_5_PENALTY=0.103187
PRIMER_RIGHT_5_PENALTY=1.668101
PRIMER_LEFT_5_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_5_SEQUENCE=TTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_5=489,20
PRIMER_RIGHT_5=1396,21
PRIMER_LEFT_5_TM=60.103
PRIMER_RIGHT_5_TM=59.332
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=908
PRIMER_PAIR_6_PENALTY=1.777107
PRIMER_LEFT_6_PENALTY=0.376636
PRIMER_RIGHT_6_PENALTY=1.400471
PRIMER_LEFT_6_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_6_SEQUENCE=TGATGTTTGTGCATTACCGAG
PRIMER_LEFT_6=614,20
PRIMER_RIGHT_6=1401,21
PRIMER_LEFT_6_TM=59.623
PRIMER_RIGHT_6_TM=59.600
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=9.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=788
PRIMER_PAIR_7_PENALTY=1.916451
PRIMER_LEFT_7_PENALTY=0.103187
PRIMER_RIGHT_7_PENALTY=1.813264
PRIMER_LEFT_7_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_7_SEQUENCE=TGTTTGTGCATTACCGAGTG
PRIMER_LEFT_7=489,20
PRIMER_RIGHT_7=1398,20
PRIMER_LEFT_7_TM=60.103
PRIMER_RIGHT_7_TM=58.187
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=910
PRIMER_PAIR_8_PENALTY=2.030485
PRIMER_LEFT_8_PENALTY=0.376636
PRIMER_RIGHT_8_PENALTY=1.653849
PRIMER_LEFT_8_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_8_SEQUENCE=TGTTGGAAAGACAGGCGTAAA
PRIMER_LEFT_8=614,20
PRIMER_RIGHT_8=1593,21
PRIMER_LEFT_8_TM=59.623
PRIMER_RIGHT_8_TM=60.654
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=6.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=980
PRIMER_PAIR_9_PENALTY=2.044738
PRIMER_LEFT_9_PENALTY=0.376636
PRIMER_RIGHT_9_PENALTY=1.668101
PRIMER_LEFT_9_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_9_SEQUENCE=TTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_9=614,20
PRIMER_RIGHT_9=1396,21
PRIMER_LEFT_9_TM=59.623
PRIMER_RIGHT_9_TM=59.332
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=783
PRIMER_PAIR_10_PENALTY=2.060424
PRIMER_LEFT_10_PENALTY=0.659953
PRIMER_RIGHT_10_PENALTY=1.400471
PRIMER_LEFT_10_SEQUENCE=GGAGCACGCAATTAGACCTT
PRIMER_RIGHT_10_SEQUENCE=TGATGTTTGTGCATTACCGAG
PRIMER_LEFT_10=491,20
PRIMER_RIGHT_10=1401,21
PRIMER_LEFT_10_TM=59.340
PRIMER_RIGHT_10_TM=59.600
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=911
PRIMER_PAIR_11_PENALTY=2.112932
PRIMER_LEFT_11_PENALTY=0.659953
PRIMER_RIGHT_11_PENALTY=1.452979
PRIMER_LEFT_11_SEQUENCE=GGAGCACGCAATTAGACCTT
PRIMER_RIGHT_11_SEQUENCE=TGATGTTTGTGCATTACCGA
PRIMER_LEFT_11=491,20
PRIMER_RIGHT_11=1401,20
PRIMER_LEFT_11_TM=59.340
PRIMER_RIGHT_11_TM=58.547
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=911
PRIMER_PAIR_12_PENALTY=2.189901
PRIMER_LEFT_12_PENALTY=0.376636
PRIMER_RIGHT_12_PENALTY=1.813264
PRIMER_LEFT_12_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_12_SEQUENCE=TGTTTGTGCATTACCGAGTG
PRIMER_LEFT_12=614,20
PRIMER_RIGHT_12=1398,20
PRIMER_LEFT_12_TM=59.623
PRIMER_RIGHT_12_TM=58.187
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=785
PRIMER_PAIR_13_PENALTY=2.192899
PRIMER_LEFT_13_PENALTY=0.902616
PRIMER_RIGHT_13_PENALTY=1.290283
PRIMER_LEFT_13_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_13_SEQUENCE=CTGTTGGAAAGACAGGCGTAA
PRIMER_LEFT_13=613,20
PRIMER_RIGHT_13=1594,21
PRIMER_LEFT_13_TM=59.097
PRIMER_RIGHT_13_TM=60.290
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=7.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=982
PRIMER_PAIR_14_PENALTY=2.214128
PRIMER_LEFT_14_PENALTY=0.103187
PRIMER_RIGHT_14_PENALTY=2.110942
PRIMER_LEFT_14_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_14_SEQUENCE=ATGATGTTTGTGCATTACCGAG
PRIMER_LEFT_14=489,20
PRIMER_RIGHT_14=1402,22
PRIMER_LEFT_14_TM=60.103
PRIMER_RIGHT_14_TM=59.889
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.909
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=914
PRIMER_PAIR_15_PENALTY=2.262863
PRIMER_LEFT_15_PENALTY=1.580178
PRIMER_RIGHT_15_PENALTY=0.682685
PRIMER_LEFT_15_SEQUENCE=ATTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_15_SEQUENCE=TGTTGGAAAGACAGGCGTAA
PRIMER_LEFT_15=612,21
PRIMER_RIGHT_15=1593,20
PRIMER_LEFT_15_TM=59.420
PRIMER_RIGHT_15_TM=59.317
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=7.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=982
PRIMER_PAIR_16_PENALTY=2.267353
PRIMER_LEFT_16_PENALTY=0.103187
PRIMER_RIGHT_16_PENALTY=2.164166
PRIMER_LEFT_16_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_16_SEQUENCE=TTGTGCATTACCGAGTGAGA
PRIMER_LEFT_16=489,20
PRIMER_RIGHT_16=1395,20
PRIMER_LEFT_16_TM=60.103
PRIMER_RIGHT_16_TM=57.836
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=907
PRIMER_PAIR_17_PENALTY=2.278862
PRIMER_LEFT_17_PENALTY=0.103187
PRIMER_RIGHT_17_PENALTY=2.175676
PRIMER_LEFT_17_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_17_SEQUENCE=GTTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_17=489,20
PRIMER_RIGHT_17=1397,22
PRIMER_LEFT_17_TM=60.103
PRIMER_RIGHT_17_TM=60.176
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=909
PRIMER_PAIR_18_PENALTY=2.303087
PRIMER_LEFT_18_PENALTY=0.902616
PRIMER_RIGHT_18_PENALTY=1.400471
PRIMER_LEFT_18_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_18_SEQUENCE=TGATGTTTGTGCATTACCGAG
PRIMER_LEFT_18=613,20
PRIMER_RIGHT_18=1401,21
PRIMER_LEFT_18_TM=59.097
PRIMER_RIGHT_18_TM=59.600
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=789
PRIMER_PAIR_19_PENALTY=2.328054
PRIMER_LEFT_19_PENALTY=0.659953
PRIMER_RIGHT_19_PENALTY=1.668101
PRIMER_LEFT_19_SEQUENCE=GGAGCACGCAATTAGACCTT
PRIMER_RIGHT_19_SEQUENCE=TTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_19=491,20
PRIMER_RIGHT_19=1396,21
PRIMER_LEFT_19_TM=59.340
PRIMER_RIGHT_19_TM=59.332
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=906
PRIMER_PAIR_20_PENALTY=2.349294
PRIMER_LEFT_20_PENALTY=0.376636
PRIMER_RIGHT_20_PENALTY=1.972658
PRIMER_LEFT_20_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_20_SEQUENCE=TTGGAAAGACAGGCGTAAAG
PRIMER_LEFT_20=614,20
PRIMER_RIGHT_20=1591,20
PRIMER_LEFT_20_TM=59.623
PRIMER_RIGHT_20_TM=58.027
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=6.3000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=978
PRIMER_PAIR_21_PENALTY=2.355595
PRIMER_LEFT_21_PENALTY=0.902616
PRIMER_RIGHT_21_PENALTY=1.452979
PRIMER_LEFT_21_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_21_SEQUENCE=TGATGTTTGTGCATTACCGA
PRIMER_LEFT_21=613,20
PRIMER_RIGHT_21=1401,20
PRIMER_LEFT_21_TM=59.097
PRIMER_RIGHT_21_TM=58.547
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=789
PRIMER_PAIR_22_PENALTY=2.473217
PRIMER_LEFT_22_PENALTY=0.659953
PRIMER_RIGHT_22_PENALTY=1.813264
PRIMER_LEFT_22_SEQUENCE=GGAGCACGCAATTAGACCTT
PRIMER_RIGHT_22_SEQUENCE=TGTTTGTGCATTACCGAGTG
PRIMER_LEFT_22=491,20
PRIMER_RIGHT_22=1398,20
PRIMER_LEFT_22_TM=59.340
PRIMER_RIGHT_22_TM=58.187
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=908
PRIMER_PAIR_23_PENALTY=2.483721
PRIMER_LEFT_23_PENALTY=0.376636
PRIMER_RIGHT_23_PENALTY=2.107085
PRIMER_LEFT_23_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_23_SEQUENCE=TTGGAAAGACAGGCGTAAAGT
PRIMER_LEFT_23=614,20
PRIMER_RIGHT_23=1591,21
PRIMER_LEFT_23_TM=59.623
PRIMER_RIGHT_23_TM=58.893
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=978
PRIMER_PAIR_24_PENALTY=2.483721
PRIMER_LEFT_24_PENALTY=0.376636
PRIMER_RIGHT_24_PENALTY=2.107085
PRIMER_LEFT_24_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_24_SEQUENCE=GTTGGAAAGACAGGCGTAAAG
PRIMER_LEFT_24=614,20
PRIMER_RIGHT_24=1592,21
PRIMER_LEFT_24_TM=59.623
PRIMER_RIGHT_24_TM=58.893
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=6.3000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=979
PRIMER_PAIR_25_PENALTY=2.487578
PRIMER_LEFT_25_PENALTY=0.376636
PRIMER_RIGHT_25_PENALTY=2.110942
PRIMER_LEFT_25_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_25_SEQUENCE=ATGATGTTTGTGCATTACCGAG
PRIMER_LEFT_25=614,20
PRIMER_RIGHT_25=1402,22
PRIMER_LEFT_25_TM=59.623
PRIMER_RIGHT_25_TM=59.889
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=789
PRIMER_PAIR_26_PENALTY=2.498405
PRIMER_LEFT_26_PENALTY=0.376636
PRIMER_RIGHT_26_PENALTY=2.121769
PRIMER_LEFT_26_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_26_SEQUENCE=GTTGGAAAGACAGGCGTAAA
PRIMER_LEFT_26=614,20
PRIMER_RIGHT_26=1592,20
PRIMER_LEFT_26_TM=59.623
PRIMER_RIGHT_26_TM=57.878
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.1000
PRIMER_RIGHT_26_END_STABILITY=6.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=979
PRIMER_PAIR_27_PENALTY=2.510348
PRIMER_LEFT_27_PENALTY=1.109878
PRIMER_RIGHT_27_PENALTY=1.400471
PRIMER_LEFT_27_SEQUENCE=TATGGAGCACGCAATTAGACC
PRIMER_RIGHT_27_SEQUENCE=TGATGTTTGTGCATTACCGAG
PRIMER_LEFT_27=488,21
PRIMER_RIGHT_27=1401,21
PRIMER_LEFT_27_TM=60.110
PRIMER_RIGHT_27_TM=59.600
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=9.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=914
PRIMER_PAIR_28_PENALTY=2.531391
PRIMER_LEFT_28_PENALTY=0.103187
PRIMER_RIGHT_28_PENALTY=2.428205
PRIMER_LEFT_28_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_28_SEQUENCE=GATGTTTGTGCATTACCGAGTG
PRIMER_LEFT_28=489,20
PRIMER_RIGHT_28=1400,22
PRIMER_LEFT_28_TM=60.103
PRIMER_RIGHT_28_TM=60.428
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=912
PRIMER_PAIR_29_PENALTY=2.535450
PRIMER_LEFT_29_PENALTY=0.103187
PRIMER_RIGHT_29_PENALTY=2.432264
PRIMER_LEFT_29_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_29_SEQUENCE=ATGTTTGTGCATTACCGAGTG
PRIMER_LEFT_29=489,20
PRIMER_RIGHT_29=1399,21
PRIMER_LEFT_29_TM=60.103
PRIMER_RIGHT_29_TM=58.568
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=911
PRIMER_PAIR_30_PENALTY=2.540803
PRIMER_LEFT_30_PENALTY=0.376636
PRIMER_RIGHT_30_PENALTY=2.164166
PRIMER_LEFT_30_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_30_SEQUENCE=TTGTGCATTACCGAGTGAGA
PRIMER_LEFT_30=614,20
PRIMER_RIGHT_30=1395,20
PRIMER_LEFT_30_TM=59.623
PRIMER_RIGHT_30_TM=57.836
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=782
PRIMER_PAIR_31_PENALTY=2.552312
PRIMER_LEFT_31_PENALTY=0.376636
PRIMER_RIGHT_31_PENALTY=2.175676
PRIMER_LEFT_31_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_31_SEQUENCE=GTTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_31=614,20
PRIMER_RIGHT_31=1397,22
PRIMER_LEFT_31_TM=59.623
PRIMER_RIGHT_31_TM=60.176
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.1000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=784
PRIMER_PAIR_32_PENALTY=2.556465
PRIMER_LEFT_32_PENALTY=0.902616
PRIMER_RIGHT_32_PENALTY=1.653849
PRIMER_LEFT_32_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_32_SEQUENCE=TGTTGGAAAGACAGGCGTAAA
PRIMER_LEFT_32=613,20
PRIMER_RIGHT_32=1593,21
PRIMER_LEFT_32_TM=59.097
PRIMER_RIGHT_32_TM=60.654
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=6.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=981
PRIMER_PAIR_33_PENALTY=2.562857
PRIMER_LEFT_33_PENALTY=1.109878
PRIMER_RIGHT_33_PENALTY=1.452979
PRIMER_LEFT_33_SEQUENCE=TATGGAGCACGCAATTAGACC
PRIMER_RIGHT_33_SEQUENCE=TGATGTTTGTGCATTACCGA
PRIMER_LEFT_33=488,21
PRIMER_RIGHT_33=1401,20
PRIMER_LEFT_33_TM=60.110
PRIMER_RIGHT_33_TM=58.547
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.6000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=914
PRIMER_PAIR_34_PENALTY=2.570718
PRIMER_LEFT_34_PENALTY=0.902616
PRIMER_RIGHT_34_PENALTY=1.668101
PRIMER_LEFT_34_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_34_SEQUENCE=TTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_34=613,20
PRIMER_RIGHT_34=1396,21
PRIMER_LEFT_34_TM=59.097
PRIMER_RIGHT_34_TM=59.332
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.4000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=784
PRIMER_PAIR_35_PENALTY=2.678981
PRIMER_LEFT_35_PENALTY=1.996296
PRIMER_RIGHT_35_PENALTY=0.682685
PRIMER_LEFT_35_SEQUENCE=TTGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_35_SEQUENCE=TGTTGGAAAGACAGGCGTAA
PRIMER_LEFT_35=613,21
PRIMER_RIGHT_35=1593,20
PRIMER_LEFT_35_TM=60.996
PRIMER_RIGHT_35_TM=59.317
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=7.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=981
PRIMER_PAIR_36_PENALTY=2.681072
PRIMER_LEFT_36_PENALTY=0.376636
PRIMER_RIGHT_36_PENALTY=2.304436
PRIMER_LEFT_36_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_36_SEQUENCE=GTTGGAAAGACAGGCGTAAAGT
PRIMER_LEFT_36=614,20
PRIMER_RIGHT_36=1592,22
PRIMER_LEFT_36_TM=59.623
PRIMER_RIGHT_36_TM=59.696
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=979
PRIMER_PAIR_37_PENALTY=2.715881
PRIMER_LEFT_37_PENALTY=0.902616
PRIMER_RIGHT_37_PENALTY=1.813264
PRIMER_LEFT_37_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_37_SEQUENCE=TGTTTGTGCATTACCGAGTG
PRIMER_LEFT_37=613,20
PRIMER_RIGHT_37=1398,20
PRIMER_LEFT_37_TM=59.097
PRIMER_RIGHT_37_TM=58.187
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=786
PRIMER_PAIR_38_PENALTY=2.756799
PRIMER_LEFT_38_PENALTY=0.103187
PRIMER_RIGHT_38_PENALTY=2.653612
PRIMER_LEFT_38_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_38_SEQUENCE=TTTGTGCATTACCGAGTGAG
PRIMER_LEFT_38=489,20
PRIMER_RIGHT_38=1396,20
PRIMER_LEFT_38_TM=60.103
PRIMER_RIGHT_38_TM=57.346
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=908
PRIMER_PAIR_39_PENALTY=2.770895
PRIMER_LEFT_39_PENALTY=0.659953
PRIMER_RIGHT_39_PENALTY=2.110942
PRIMER_LEFT_39_SEQUENCE=GGAGCACGCAATTAGACCTT
PRIMER_RIGHT_39_SEQUENCE=ATGATGTTTGTGCATTACCGAG
PRIMER_LEFT_39=491,20
PRIMER_RIGHT_39=1402,22
PRIMER_LEFT_39_TM=59.340
PRIMER_RIGHT_39_TM=59.889
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=9.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=912
PRIMER_PAIR_40_PENALTY=2.777979
PRIMER_LEFT_40_PENALTY=1.109878
PRIMER_RIGHT_40_PENALTY=1.668101
PRIMER_LEFT_40_SEQUENCE=TATGGAGCACGCAATTAGACC
PRIMER_RIGHT_40_SEQUENCE=TTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_40=488,21
PRIMER_RIGHT_40=1396,21
PRIMER_LEFT_40_TM=60.110
PRIMER_RIGHT_40_TM=59.332
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=909
PRIMER_PAIR_41_PENALTY=2.804841
PRIMER_LEFT_41_PENALTY=0.376636
PRIMER_RIGHT_41_PENALTY=2.428205
PRIMER_LEFT_41_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_41_SEQUENCE=GATGTTTGTGCATTACCGAGTG
PRIMER_LEFT_41=614,20
PRIMER_RIGHT_41=1400,22
PRIMER_LEFT_41_TM=59.623
PRIMER_RIGHT_41_TM=60.428
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=787
PRIMER_PAIR_42_PENALTY=2.806712
PRIMER_LEFT_42_PENALTY=0.103187
PRIMER_RIGHT_42_PENALTY=2.703526
PRIMER_LEFT_42_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_42_SEQUENCE=GTTTGTGCATTACCGAGTGAG
PRIMER_LEFT_42=489,20
PRIMER_RIGHT_42=1397,21
PRIMER_LEFT_42_TM=60.103
PRIMER_RIGHT_42_TM=58.296
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=909
PRIMER_PAIR_43_PENALTY=2.808900
PRIMER_LEFT_43_PENALTY=0.376636
PRIMER_RIGHT_43_PENALTY=2.432264
PRIMER_LEFT_43_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_43_SEQUENCE=ATGTTTGTGCATTACCGAGTG
PRIMER_LEFT_43=614,20
PRIMER_RIGHT_43=1399,21
PRIMER_LEFT_43_TM=59.623
PRIMER_RIGHT_43_TM=58.568
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=786
PRIMER_PAIR_44_PENALTY=2.824119
PRIMER_LEFT_44_PENALTY=0.659953
PRIMER_RIGHT_44_PENALTY=2.164166
PRIMER_LEFT_44_SEQUENCE=GGAGCACGCAATTAGACCTT
PRIMER_RIGHT_44_SEQUENCE=TTGTGCATTACCGAGTGAGA
PRIMER_LEFT_44=491,20
PRIMER_RIGHT_44=1395,20
PRIMER_LEFT_44_TM=59.340
PRIMER_RIGHT_44_TM=57.836
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=905
PRIMER_PAIR_45_PENALTY=2.835629
PRIMER_LEFT_45_PENALTY=0.659953
PRIMER_RIGHT_45_PENALTY=2.175676
PRIMER_LEFT_45_SEQUENCE=GGAGCACGCAATTAGACCTT
PRIMER_RIGHT_45_SEQUENCE=GTTTGTGCATTACCGAGTGAGA
PRIMER_LEFT_45=491,20
PRIMER_RIGHT_45=1397,22
PRIMER_LEFT_45_TM=59.340
PRIMER_RIGHT_45_TM=60.176
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=907
PRIMER_PAIR_46_PENALTY=2.864956
PRIMER_LEFT_46_PENALTY=0.103187
PRIMER_RIGHT_46_PENALTY=2.761770
PRIMER_LEFT_46_SEQUENCE=ATGGAGCACGCAATTAGACC
PRIMER_RIGHT_46_SEQUENCE=GATGATGTTTGTGCATTACCGA
PRIMER_LEFT_46=489,20
PRIMER_RIGHT_46=1403,22
PRIMER_LEFT_46_TM=60.103
PRIMER_RIGHT_46_TM=60.762
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.6000
PRIMER_RIGHT_46_END_STABILITY=9.6000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=915
PRIMER_PAIR_47_PENALTY=2.870461
PRIMER_LEFT_47_PENALTY=1.580178
PRIMER_RIGHT_47_PENALTY=1.290283
PRIMER_LEFT_47_SEQUENCE=ATTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_47_SEQUENCE=CTGTTGGAAAGACAGGCGTAA
PRIMER_LEFT_47=612,21
PRIMER_RIGHT_47=1594,21
PRIMER_LEFT_47_TM=59.420
PRIMER_RIGHT_47_TM=60.290
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.4000
PRIMER_RIGHT_47_END_STABILITY=7.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=983
PRIMER_PAIR_48_PENALTY=2.875274
PRIMER_LEFT_48_PENALTY=0.902616
PRIMER_RIGHT_48_PENALTY=1.972658
PRIMER_LEFT_48_SEQUENCE=TTGTGTATGGGTGGAAATGG
PRIMER_RIGHT_48_SEQUENCE=TTGGAAAGACAGGCGTAAAG
PRIMER_LEFT_48=613,20
PRIMER_RIGHT_48=1591,20
PRIMER_LEFT_48_TM=59.097
PRIMER_RIGHT_48_TM=58.027
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=979
PRIMER_PAIR_49_PENALTY=2.893195
PRIMER_LEFT_49_PENALTY=0.376636
PRIMER_RIGHT_49_PENALTY=2.516559
PRIMER_LEFT_49_SEQUENCE=TGTGTATGGGTGGAAATGGA
PRIMER_RIGHT_49_SEQUENCE=TGGAAAGACAGGCGTAAAGT
PRIMER_LEFT_49=614,20
PRIMER_RIGHT_49=1590,20
PRIMER_LEFT_49_TM=59.623
PRIMER_RIGHT_49_TM=57.483
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=977
=
SEQUENCE_ID=MAGIC.329_4_4574480_4574875_ler-0_edi-0_69707_29514_TRUE
SEQUENCE_TARGET=950,495
SEQUENCE_TEMPLATE=TTTTTGAAACCAAGTGGTCATATTCATNAGAATTAACACCAAGTATTGGTTTCACTTCAACCTNCAAATATATCNNTCGCAAAATTGTGAGGTANTTGCNAACCGAAAACCCTAAATATTGTTATAACAGAGAAGAGATTCGNTAAANAAANANAATCGAACAAACCCAAATATNAAACTTTAGTNTCATAAANTTNTAACCTTCAATTGATTCCTTTGCTGGAGAAACAAAATTTCTTCAANTCTAAGAAAGAAGACACANTGAGAGAGACGANANTTTTCATAGACCANTCCTATTTTCTCACCAAAGNACATTGANNNNNTNTTCTGCNNNGATACACAAGTGTTTCNANTGCAGGTNTCATTACATGATCAATTCTACCTTCANGTACTTGTTCCACTTCAATCCATCCNTGACTCTAATCCTTCTTGCTNATTTTATACCCTTTGATAACAAGATCAAAANNATGTAATAGATCCTTGTCTTTAGGATTCATNNGTGGNNNNNNNNNNTCATATTCATGAGAATTCACACCTNNNAAAAGGTCCCTCGNAAATTTGCTACAGAATTGNCNGGACTTTGCGAGCAGATAGGAGAACGTCGCTAATTCNTTNCAAATTTTGCGAGGAATATATCCCTCGCAAANNNNNNNNNNNNNNNNNNATTTTCTTCTTNTCTAGTAGAACTNGCAGAAACTCTTTCGATCGCTAAATAGTCCTCCANCTTCTAACTTTGTACCTAAGATCTTTACANTTTTCATAGACAAGTCCTATTTTCNCACCAAAGGACATTGATTTCCTTTTNNNNNNNNNNNCACAAGTGTTTCAATTGCAGGTTTCATTACATGATCAATTCTACCTTNATGTACTTGTTTNACTTCAATCCATCCNTGACTCCAATCNTTCNTGCTCATTTTATANCCTTTGTTCACAAGATCAAAAATANGTAACAGATNCTTGTCTTTANAATTCTTTTTTGAAACCAAGTGGTCATATTCATNAGAATTAATACCAACTATTGGTTTCACTTCAACCTGCAAATATATNNCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCNTAGATATTGNTATAACANAGAAGANATTCGACAAAAANAAATAAANTCGAACAAACCCAAATATCAAACTTTAGTGTCATAAATTTGTTACCTTCAATTGATTCCTTTGCTGGAGANACNAAATTTCTTCAAANTGTTCTAAGNAAGAAGACACANTGAGANAGACGANAGTTTCTCNNAAATTTTTNTTTTACTTTTTGTTACNNTCACANTTNCNNNNTTNTTTTTTTTTTTTTTNGTTTTNAGTGAANTTTNNGCTTTAGTGAATTTNTACTAAAACGGTTNTNTTTCGAAAATAAGAGGTCTAGGCNCAATTACTANCANTTAAAGTGTTAACATTANANTAACTTTTTCAACGCTTTAAAATTCTTTTAGAAACCTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_15164230_15164769_edi-0_wil-2_562716_346580_TRUE
SEQUENCE_TARGET=950,639
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_14469454_14470123_no-0_sf-2_14222_5021_TRUE
SEQUENCE_TARGET=950,769
SEQUENCE_TEMPLATE=ATGNTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANANTANAATTCAGGATGATGNNGCTGATAANAATCAATTGAGGANAAAGAGGAAGNGNAAAGTTNTTGACGNTTGCCCCNNGAAAGTNNNGACNTATCCNGAACAAGAAAAAAACAGTNGGAGTTTNGTCAAGTTTNNATTTTAANNAACTTAAGATTTTTGGTGTTGAATGAGTNAGAACNTGTTGAAGAAGACCTGGAAAATGANAAAAGAGAGGACNANATANAAGGTTTGGAAGAAGAGGANNANCTNGAAGATAAAGTANANGATAAAANAAGGNNGGGNNNTGTAACAGAGATGGANAAGGNTAATAAGGATGCANANATAACTCTNAANATGNAANTCAAANAGGNAAAGTCGCTCCNNCAAGNCCATCACTACACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTGCAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_5925330_5925500_ct-1_bur-0_18235_91460_TRUE
SEQUENCE_TARGET=950,270
SEQUENCE_TEMPLATE=TTAATNTTAAACATGGCGGCNGACCTTCTTCTTCTTCNTTAAGACAANACATAATGANAGAAGNAAACTGGGGAAGATGAAGATGGAGTGGTGAANAACAAAACNGNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATACCAATCCTGGTTTAAATTNAAGGGNNGAATAAACCAANNATTTCATAGGCCCATGAAAGCCCAATAGAAATTACTGTGNCATNNTTATGAGATTNTCGCGTGGCTTNTTGTTGANTTNTAACGTTTCGGCTTCNCGACCTCAACCGTCTTCTGCTCCTCCGCCCCGCCACAGTTTNTTAGGGTTCTTCCGCATTGTTTCTCCGAATTGCAATCCCCTNATTCTCCACAATNTAAGNTTCGTTTATTTTGTCTTCANTTTCTTCTNNANNNNNNNNNNNNNNNNNTNCNTTGTTCTCTCTTNCAGAATCATCGANNNNNNNNNNNNNNNNTTTATCCGATTCTGATTTGTATACCTNTCNNCTTCNTTTGTTAATNTGTGTGCTGATTTAGCTTCCTNACTCTNNTGNTNNATNNNGNNNANTAGCTCTTTTTCTTTTCGTGGTGAATACTAATGATGCAAAGCTTTGAATCTGATCCATGNATTNTTTTATATGCTGCAGAAACAGANAGCTAATATAATTGAGTTTGGNTACTCGCAAGTGAAAATATGTCGGTCACAGCGGGAGTGAGTGAATCTGCAATTGCTGTAAGGGAGAAACTAAAAGGTGGTATTGGACAGACNAAAGTNNGAAGGTATTGGCCAGGAAAAGCTCCGGAGTGGGCTGAGGAAGCTGAAGAAGATGATGATGTTAGGATGCAGAAGGTTTCTGTTTTGGATAGAGCTTTTCCAAAGNATGATGATTTGGGTGTTGCTAGGAAGGATGATCCAAGGCTGCGCCGTTTAGCTAAGACTAAAGTTGAAAACCGTGACGAAGTTAGAGCTGATCATCGGCGGATNAGACAGGCTGAGATTATTTNTACGGAAGAAGAAGAATCGAGGAATCAAGAGAATAGAGACGAGGANGATGATGAAGATGCNNTGGAAGANAGAAGAAGAAGNATTAGAGAGAAAAATCTTAGAAGAGCGCAAGAGGAGGCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATTGGGAGAGGAAGAATCCGAAACCTTNNTCAGCTCAACCNAAAAAGAAATGGAACTTTATGCAGAAATATTACCACAAGGGTGCCTTCTTCCAGGCAGATCCTGANGATGAGGCAGGTTCTGCTGGAACCGATGGTATATTTCAGCGCGACTTCTCTGCTCCAACTGGAGAAGATAGGTTGGANAAATCGATTCTCCCCAAAGTTATGCAAGTCAAGCACTTTGGTCGTAGTGGAAGAACTAAATGGACTCACCTTGTCAATGAGGACACAACAGATTGGAGTAACCCGTAAGTTTTCTGATTTCAGNGTTTCTTTTCTTGCNNTTNGTTAGGNANAGACTCGTTTAGGTNTCTGAAATATGATTAATAGCNTNTGAGTNGAAACATTAAGATGAGAACTGAANTAAATCAGTGCATGCAAATGTGAACCCGGGTNGTGTTGGGAGAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.816342
PRIMER_LEFT_0_PENALTY=0.408401
PRIMER_RIGHT_0_PENALTY=0.407941
PRIMER_LEFT_0_SEQUENCE=AGCGGGAGTGAGTGAATCTG
PRIMER_RIGHT_0_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_0=825,20
PRIMER_RIGHT_0=1810,20
PRIMER_LEFT_0_TM=60.408
PRIMER_RIGHT_0_TM=59.592
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=986
PRIMER_PAIR_1_PENALTY=0.816342
PRIMER_LEFT_1_PENALTY=0.408401
PRIMER_RIGHT_1_PENALTY=0.407941
PRIMER_LEFT_1_SEQUENCE=CAGCGGGAGTGAGTGAATCT
PRIMER_RIGHT_1_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_1=824,20
PRIMER_RIGHT_1=1810,20
PRIMER_LEFT_1_TM=60.408
PRIMER_RIGHT_1_TM=59.592
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=987
PRIMER_PAIR_2_PENALTY=1.099504
PRIMER_LEFT_2_PENALTY=0.691563
PRIMER_RIGHT_2_PENALTY=0.407941
PRIMER_LEFT_2_SEQUENCE=GGCTGAGGAAGCTGAAGAAG
PRIMER_RIGHT_2_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_2=927,20
PRIMER_RIGHT_2=1810,20
PRIMER_LEFT_2_TM=59.308
PRIMER_RIGHT_2_TM=59.592
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=884
PRIMER_PAIR_3_PENALTY=1.334845
PRIMER_LEFT_3_PENALTY=0.818184
PRIMER_RIGHT_3_PENALTY=0.516662
PRIMER_LEFT_3_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_3_SEQUENCE=TTTCGGATTCTTCCTCTCCC
PRIMER_LEFT_3=801,20
PRIMER_RIGHT_3=1743,20
PRIMER_LEFT_3_TM=59.182
PRIMER_RIGHT_3_TM=60.517
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=943
PRIMER_PAIR_4_PENALTY=1.455593
PRIMER_LEFT_4_PENALTY=1.047652
PRIMER_RIGHT_4_PENALTY=0.407941
PRIMER_LEFT_4_SEQUENCE=GAAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_4_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_4=896,21
PRIMER_RIGHT_4=1810,20
PRIMER_LEFT_4_TM=59.952
PRIMER_RIGHT_4_TM=59.592
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=915
PRIMER_PAIR_5_PENALTY=1.527191
PRIMER_LEFT_5_PENALTY=1.010529
PRIMER_RIGHT_5_PENALTY=0.516662
PRIMER_LEFT_5_SEQUENCE=CGCAAGTGAAAATATGTCGGT
PRIMER_RIGHT_5_SEQUENCE=TTTCGGATTCTTCCTCTCCC
PRIMER_LEFT_5=801,21
PRIMER_RIGHT_5=1743,20
PRIMER_LEFT_5_TM=60.011
PRIMER_RIGHT_5_TM=60.517
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=943
PRIMER_PAIR_6_PENALTY=1.564314
PRIMER_LEFT_6_PENALTY=1.047652
PRIMER_RIGHT_6_PENALTY=0.516662
PRIMER_LEFT_6_SEQUENCE=GAAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_6_SEQUENCE=TTTCGGATTCTTCCTCTCCC
PRIMER_LEFT_6=896,21
PRIMER_RIGHT_6=1743,20
PRIMER_LEFT_6_TM=59.952
PRIMER_RIGHT_6_TM=60.517
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=848
PRIMER_PAIR_7_PENALTY=1.839525
PRIMER_LEFT_7_PENALTY=1.431584
PRIMER_RIGHT_7_PENALTY=0.407941
PRIMER_LEFT_7_SEQUENCE=TATTGGCCAGGAAAAGCTCC
PRIMER_RIGHT_7_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_7=901,20
PRIMER_RIGHT_7=1810,20
PRIMER_LEFT_7_TM=61.432
PRIMER_RIGHT_7_TM=59.592
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=910
PRIMER_PAIR_8_PENALTY=2.220391
PRIMER_LEFT_8_PENALTY=1.812450
PRIMER_RIGHT_8_PENALTY=0.407941
PRIMER_LEFT_8_SEQUENCE=AAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_8_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_8=897,20
PRIMER_RIGHT_8=1810,20
PRIMER_LEFT_8_TM=58.188
PRIMER_RIGHT_8_TM=59.592
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=914
PRIMER_PAIR_9_PENALTY=2.297335
PRIMER_LEFT_9_PENALTY=0.408401
PRIMER_RIGHT_9_PENALTY=1.888934
PRIMER_LEFT_9_SEQUENCE=AGCGGGAGTGAGTGAATCTG
PRIMER_RIGHT_9_SEQUENCE=AAGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_9=825,20
PRIMER_RIGHT_9=1811,21
PRIMER_LEFT_9_TM=60.408
PRIMER_RIGHT_9_TM=60.889
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=987
PRIMER_PAIR_10_PENALTY=2.297335
PRIMER_LEFT_10_PENALTY=0.408401
PRIMER_RIGHT_10_PENALTY=1.888934
PRIMER_LEFT_10_SEQUENCE=CAGCGGGAGTGAGTGAATCT
PRIMER_RIGHT_10_SEQUENCE=AAGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_10=824,20
PRIMER_RIGHT_10=1811,21
PRIMER_LEFT_10_TM=60.408
PRIMER_RIGHT_10_TM=60.889
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=7.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=988
PRIMER_PAIR_11_PENALTY=2.329112
PRIMER_LEFT_11_PENALTY=1.812450
PRIMER_RIGHT_11_PENALTY=0.516662
PRIMER_LEFT_11_SEQUENCE=AAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_11_SEQUENCE=TTTCGGATTCTTCCTCTCCC
PRIMER_LEFT_11=897,20
PRIMER_RIGHT_11=1743,20
PRIMER_LEFT_11_TM=58.188
PRIMER_RIGHT_11_TM=60.517
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=847
PRIMER_PAIR_12_PENALTY=2.346165
PRIMER_LEFT_12_PENALTY=1.938224
PRIMER_RIGHT_12_PENALTY=0.407941
PRIMER_LEFT_12_SEQUENCE=GGGCTGAGGAAGCTGAAGAA
PRIMER_RIGHT_12_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_12=926,20
PRIMER_RIGHT_12=1810,20
PRIMER_LEFT_12_TM=61.938
PRIMER_RIGHT_12_TM=59.592
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=885
PRIMER_PAIR_13_PENALTY=2.381431
PRIMER_LEFT_13_PENALTY=0.691563
PRIMER_RIGHT_13_PENALTY=1.689868
PRIMER_LEFT_13_SEQUENCE=GGCTGAGGAAGCTGAAGAAG
PRIMER_RIGHT_13_SEQUENCE=AATATACCATCGGTTCCAGCA
PRIMER_LEFT_13=927,20
PRIMER_RIGHT_13=1862,21
PRIMER_LEFT_13_TM=59.308
PRIMER_RIGHT_13_TM=59.310
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=936
PRIMER_PAIR_14_PENALTY=2.520365
PRIMER_LEFT_14_PENALTY=2.003703
PRIMER_RIGHT_14_PENALTY=0.516662
PRIMER_LEFT_14_SEQUENCE=TCGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_14_SEQUENCE=TTTCGGATTCTTCCTCTCCC
PRIMER_LEFT_14=800,21
PRIMER_RIGHT_14=1743,20
PRIMER_LEFT_14_TM=61.004
PRIMER_RIGHT_14_TM=60.517
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=9.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=944
PRIMER_PAIR_15_PENALTY=2.565210
PRIMER_LEFT_15_PENALTY=2.157269
PRIMER_RIGHT_15_PENALTY=0.407941
PRIMER_LEFT_15_SEQUENCE=GGCTGAGGAAGCTGAAGAAGA
PRIMER_RIGHT_15_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_15=927,21
PRIMER_RIGHT_15=1810,20
PRIMER_LEFT_15_TM=61.157
PRIMER_RIGHT_15_TM=59.592
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=884
PRIMER_PAIR_16_PENALTY=2.580497
PRIMER_LEFT_16_PENALTY=0.691563
PRIMER_RIGHT_16_PENALTY=1.888934
PRIMER_LEFT_16_SEQUENCE=GGCTGAGGAAGCTGAAGAAG
PRIMER_RIGHT_16_SEQUENCE=AAGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_16=927,20
PRIMER_RIGHT_16=1811,21
PRIMER_LEFT_16_TM=59.308
PRIMER_RIGHT_16_TM=60.889
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=885
PRIMER_PAIR_17_PENALTY=2.643118
PRIMER_LEFT_17_PENALTY=2.235177
PRIMER_RIGHT_17_PENALTY=0.407941
PRIMER_LEFT_17_SEQUENCE=ACAGCGGGAGTGAGTGAATCT
PRIMER_RIGHT_17_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_17=823,21
PRIMER_RIGHT_17=1810,20
PRIMER_LEFT_17_TM=61.235
PRIMER_RIGHT_17_TM=59.592
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=988
PRIMER_PAIR_18_PENALTY=2.737520
PRIMER_LEFT_18_PENALTY=1.047652
PRIMER_RIGHT_18_PENALTY=1.689868
PRIMER_LEFT_18_SEQUENCE=GAAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_18_SEQUENCE=AATATACCATCGGTTCCAGCA
PRIMER_LEFT_18=896,21
PRIMER_RIGHT_18=1862,21
PRIMER_LEFT_18_TM=59.952
PRIMER_RIGHT_18_TM=59.310
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=967
PRIMER_PAIR_19_PENALTY=2.776857
PRIMER_LEFT_19_PENALTY=0.691563
PRIMER_RIGHT_19_PENALTY=2.085294
PRIMER_LEFT_19_SEQUENCE=GGCTGAGGAAGCTGAAGAAG
PRIMER_RIGHT_19_SEQUENCE=ATATACCATCGGTTCCAGCA
PRIMER_LEFT_19=927,20
PRIMER_RIGHT_19=1861,20
PRIMER_LEFT_19_TM=59.308
PRIMER_RIGHT_19_TM=57.915
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=935
PRIMER_PAIR_20_PENALTY=2.802930
PRIMER_LEFT_20_PENALTY=0.408401
PRIMER_RIGHT_20_PENALTY=2.394529
PRIMER_LEFT_20_SEQUENCE=AGCGGGAGTGAGTGAATCTG
PRIMER_RIGHT_20_SEQUENCE=GGATTCTTCCTCTCCCAATCT
PRIMER_LEFT_20=825,20
PRIMER_RIGHT_20=1739,21
PRIMER_LEFT_20_TM=60.408
PRIMER_RIGHT_20_TM=58.605
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=7.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=915
PRIMER_PAIR_21_PENALTY=2.819592
PRIMER_LEFT_21_PENALTY=0.818184
PRIMER_RIGHT_21_PENALTY=2.001409
PRIMER_LEFT_21_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_21_SEQUENCE=TCGGATTCTTCCTCTCCCAA
PRIMER_LEFT_21=801,20
PRIMER_RIGHT_21=1741,20
PRIMER_LEFT_21_TM=59.182
PRIMER_RIGHT_21_TM=62.001
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=10.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=941
PRIMER_PAIR_22_PENALTY=2.819592
PRIMER_LEFT_22_PENALTY=0.818184
PRIMER_RIGHT_22_PENALTY=2.001409
PRIMER_LEFT_22_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_22_SEQUENCE=TTCGGATTCTTCCTCTCCCA
PRIMER_LEFT_22=801,20
PRIMER_RIGHT_22=1742,20
PRIMER_LEFT_22_TM=59.182
PRIMER_RIGHT_22_TM=62.001
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=942
PRIMER_PAIR_23_PENALTY=2.821608
PRIMER_LEFT_23_PENALTY=2.413667
PRIMER_RIGHT_23_PENALTY=0.407941
PRIMER_LEFT_23_SEQUENCE=AGTGGGCTGAGGAAGCTGAA
PRIMER_RIGHT_23_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_23=923,20
PRIMER_RIGHT_23=1810,20
PRIMER_LEFT_23_TM=62.414
PRIMER_RIGHT_23_TM=59.592
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=888
PRIMER_PAIR_24_PENALTY=2.851382
PRIMER_LEFT_24_PENALTY=0.408401
PRIMER_RIGHT_24_PENALTY=2.442981
PRIMER_LEFT_24_SEQUENCE=AGCGGGAGTGAGTGAATCTG
PRIMER_RIGHT_24_SEQUENCE=GGATTCTTCCTCTCCCAATC
PRIMER_LEFT_24=825,20
PRIMER_RIGHT_24=1739,20
PRIMER_LEFT_24_TM=60.408
PRIMER_RIGHT_24_TM=57.557
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=7.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=915
PRIMER_PAIR_25_PENALTY=2.893425
PRIMER_LEFT_25_PENALTY=0.818184
PRIMER_RIGHT_25_PENALTY=2.075241
PRIMER_LEFT_25_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_25_SEQUENCE=AAGGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_25=801,20
PRIMER_RIGHT_25=1747,22
PRIMER_LEFT_25_TM=59.182
PRIMER_RIGHT_25_TM=60.075
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=947
PRIMER_PAIR_26_PENALTY=2.926280
PRIMER_LEFT_26_PENALTY=2.518339
PRIMER_RIGHT_26_PENALTY=0.407941
PRIMER_LEFT_26_SEQUENCE=GCTGAGGAAGCTGAAGAAGA
PRIMER_RIGHT_26_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_26=928,20
PRIMER_RIGHT_26=1810,20
PRIMER_LEFT_26_TM=57.482
PRIMER_RIGHT_26_TM=59.592
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=7.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=883
PRIMER_PAIR_27_PENALTY=2.932659
PRIMER_LEFT_27_PENALTY=2.524718
PRIMER_RIGHT_27_PENALTY=0.407941
PRIMER_LEFT_27_SEQUENCE=GTCACAGCGGGAGTGAGTGA
PRIMER_RIGHT_27_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_27=820,20
PRIMER_RIGHT_27=1810,20
PRIMER_LEFT_27_TM=62.525
PRIMER_RIGHT_27_TM=59.592
PRIMER_LEFT_27_GC_PERCENT=60.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=7.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=991
PRIMER_PAIR_28_PENALTY=2.936586
PRIMER_LEFT_28_PENALTY=1.047652
PRIMER_RIGHT_28_PENALTY=1.888934
PRIMER_LEFT_28_SEQUENCE=GAAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_28_SEQUENCE=AAGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_28=896,21
PRIMER_RIGHT_28=1811,21
PRIMER_LEFT_28_TM=59.952
PRIMER_RIGHT_28_TM=60.889
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=7.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=916
PRIMER_PAIR_29_PENALTY=2.987774
PRIMER_LEFT_29_PENALTY=0.691563
PRIMER_RIGHT_29_PENALTY=2.296211
PRIMER_LEFT_29_SEQUENCE=GGCTGAGGAAGCTGAAGAAG
PRIMER_RIGHT_29_SEQUENCE=GTTCCAGCAGAACCTGCCTC
PRIMER_LEFT_29=927,20
PRIMER_RIGHT_29=1850,20
PRIMER_LEFT_29_TM=59.308
PRIMER_RIGHT_29_TM=62.296
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=60.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=9.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=924
PRIMER_PAIR_30_PENALTY=3.011938
PRIMER_LEFT_30_PENALTY=1.010529
PRIMER_RIGHT_30_PENALTY=2.001409
PRIMER_LEFT_30_SEQUENCE=CGCAAGTGAAAATATGTCGGT
PRIMER_RIGHT_30_SEQUENCE=TCGGATTCTTCCTCTCCCAA
PRIMER_LEFT_30=801,21
PRIMER_RIGHT_30=1741,20
PRIMER_LEFT_30_TM=60.011
PRIMER_RIGHT_30_TM=62.001
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=10.0000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=941
PRIMER_PAIR_31_PENALTY=3.011938
PRIMER_LEFT_31_PENALTY=1.010529
PRIMER_RIGHT_31_PENALTY=2.001409
PRIMER_LEFT_31_SEQUENCE=CGCAAGTGAAAATATGTCGGT
PRIMER_RIGHT_31_SEQUENCE=TTCGGATTCTTCCTCTCCCA
PRIMER_LEFT_31=801,21
PRIMER_RIGHT_31=1742,20
PRIMER_LEFT_31_TM=60.011
PRIMER_RIGHT_31_TM=62.001
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=942
PRIMER_PAIR_32_PENALTY=3.024339
PRIMER_LEFT_32_PENALTY=0.818184
PRIMER_RIGHT_32_PENALTY=2.206156
PRIMER_LEFT_32_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_32_SEQUENCE=AGGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_32=801,20
PRIMER_RIGHT_32=1746,21
PRIMER_LEFT_32_TM=59.182
PRIMER_RIGHT_32_TM=58.794
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.6000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=946
PRIMER_PAIR_33_PENALTY=3.050054
PRIMER_LEFT_33_PENALTY=0.818184
PRIMER_RIGHT_33_PENALTY=2.231870
PRIMER_LEFT_33_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_33_SEQUENCE=GTTTCGGATTCTTCCTCTCC
PRIMER_LEFT_33=801,20
PRIMER_RIGHT_33=1744,20
PRIMER_LEFT_33_TM=59.182
PRIMER_RIGHT_33_TM=57.768
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.6000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=944
PRIMER_PAIR_34_PENALTY=3.050054
PRIMER_LEFT_34_PENALTY=0.818184
PRIMER_RIGHT_34_PENALTY=2.231870
PRIMER_LEFT_34_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_34_SEQUENCE=GGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_34=801,20
PRIMER_RIGHT_34=1745,20
PRIMER_LEFT_34_TM=59.182
PRIMER_RIGHT_34_TM=57.768
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.6000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=945
PRIMER_PAIR_35_PENALTY=3.057950
PRIMER_LEFT_35_PENALTY=2.650009
PRIMER_RIGHT_35_PENALTY=0.407941
PRIMER_LEFT_35_SEQUENCE=GCTGAGGAAGCTGAAGAAGATG
PRIMER_RIGHT_35_SEQUENCE=AGAAGGCACCCTTGTGGTAA
PRIMER_LEFT_35=928,22
PRIMER_RIGHT_35=1810,20
PRIMER_LEFT_35_TM=60.650
PRIMER_RIGHT_35_TM=59.592
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=7.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=883
PRIMER_PAIR_36_PENALTY=3.069431
PRIMER_LEFT_36_PENALTY=0.408401
PRIMER_RIGHT_36_PENALTY=2.661030
PRIMER_LEFT_36_SEQUENCE=CAGCGGGAGTGAGTGAATCT
PRIMER_RIGHT_36_SEQUENCE=AAGGTTTCGGATTCTTCCTCT
PRIMER_LEFT_36=824,20
PRIMER_RIGHT_36=1747,21
PRIMER_LEFT_36_TM=60.408
PRIMER_RIGHT_36_TM=58.339
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=924
PRIMER_PAIR_37_PENALTY=3.085770
PRIMER_LEFT_37_PENALTY=1.010529
PRIMER_RIGHT_37_PENALTY=2.075241
PRIMER_LEFT_37_SEQUENCE=CGCAAGTGAAAATATGTCGGT
PRIMER_RIGHT_37_SEQUENCE=AAGGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_37=801,21
PRIMER_RIGHT_37=1747,22
PRIMER_LEFT_37_TM=60.011
PRIMER_RIGHT_37_TM=60.075
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=947
PRIMER_PAIR_38_PENALTY=3.101343
PRIMER_LEFT_38_PENALTY=0.818184
PRIMER_RIGHT_38_PENALTY=2.283160
PRIMER_LEFT_38_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_38_SEQUENCE=GTTTCGGATTCTTCCTCTCCC
PRIMER_LEFT_38=801,20
PRIMER_RIGHT_38=1744,21
PRIMER_LEFT_38_TM=59.182
PRIMER_RIGHT_38_TM=61.283
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=944
PRIMER_PAIR_39_PENALTY=3.101343
PRIMER_LEFT_39_PENALTY=0.818184
PRIMER_RIGHT_39_PENALTY=2.283160
PRIMER_LEFT_39_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_39_SEQUENCE=GGTTTCGGATTCTTCCTCTCC
PRIMER_LEFT_39=801,20
PRIMER_RIGHT_39=1745,21
PRIMER_LEFT_39_TM=59.182
PRIMER_RIGHT_39_TM=61.283
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=945
PRIMER_PAIR_40_PENALTY=3.113158
PRIMER_LEFT_40_PENALTY=0.691563
PRIMER_RIGHT_40_PENALTY=2.421595
PRIMER_LEFT_40_SEQUENCE=GGCTGAGGAAGCTGAAGAAG
PRIMER_RIGHT_40_SEQUENCE=TCCAGTTGGAGCAGAGAAGT
PRIMER_LEFT_40=927,20
PRIMER_RIGHT_40=1890,20
PRIMER_LEFT_40_TM=59.308
PRIMER_RIGHT_40_TM=57.578
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=964
PRIMER_PAIR_41_PENALTY=3.121452
PRIMER_LEFT_41_PENALTY=1.431584
PRIMER_RIGHT_41_PENALTY=1.689868
PRIMER_LEFT_41_SEQUENCE=TATTGGCCAGGAAAAGCTCC
PRIMER_RIGHT_41_SEQUENCE=AATATACCATCGGTTCCAGCA
PRIMER_LEFT_41=901,20
PRIMER_RIGHT_41=1862,21
PRIMER_LEFT_41_TM=61.432
PRIMER_RIGHT_41_TM=59.310
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=962
PRIMER_PAIR_42_PENALTY=3.122893
PRIMER_LEFT_42_PENALTY=1.047652
PRIMER_RIGHT_42_PENALTY=2.075241
PRIMER_LEFT_42_SEQUENCE=GAAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_42_SEQUENCE=AAGGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_42=896,21
PRIMER_RIGHT_42=1747,22
PRIMER_LEFT_42_TM=59.952
PRIMER_RIGHT_42_TM=60.075
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=852
PRIMER_PAIR_43_PENALTY=3.132946
PRIMER_LEFT_43_PENALTY=1.047652
PRIMER_RIGHT_43_PENALTY=2.085294
PRIMER_LEFT_43_SEQUENCE=GAAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_43_SEQUENCE=ATATACCATCGGTTCCAGCA
PRIMER_LEFT_43=896,21
PRIMER_RIGHT_43=1861,20
PRIMER_LEFT_43_TM=59.952
PRIMER_RIGHT_43_TM=57.915
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=966
PRIMER_PAIR_44_PENALTY=3.212712
PRIMER_LEFT_44_PENALTY=0.818184
PRIMER_RIGHT_44_PENALTY=2.394529
PRIMER_LEFT_44_SEQUENCE=CGCAAGTGAAAATATGTCGG
PRIMER_RIGHT_44_SEQUENCE=GGATTCTTCCTCTCCCAATCT
PRIMER_LEFT_44=801,20
PRIMER_RIGHT_44=1739,21
PRIMER_LEFT_44_TM=59.182
PRIMER_RIGHT_44_TM=58.605
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=939
PRIMER_PAIR_45_PENALTY=3.216684
PRIMER_LEFT_45_PENALTY=1.010529
PRIMER_RIGHT_45_PENALTY=2.206156
PRIMER_LEFT_45_SEQUENCE=CGCAAGTGAAAATATGTCGGT
PRIMER_RIGHT_45_SEQUENCE=AGGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_45=801,21
PRIMER_RIGHT_45=1746,21
PRIMER_LEFT_45_TM=60.011
PRIMER_RIGHT_45_TM=58.794
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=946
PRIMER_PAIR_46_PENALTY=3.242399
PRIMER_LEFT_46_PENALTY=1.010529
PRIMER_RIGHT_46_PENALTY=2.231870
PRIMER_LEFT_46_SEQUENCE=CGCAAGTGAAAATATGTCGGT
PRIMER_RIGHT_46_SEQUENCE=GTTTCGGATTCTTCCTCTCC
PRIMER_LEFT_46=801,21
PRIMER_RIGHT_46=1744,20
PRIMER_LEFT_46_TM=60.011
PRIMER_RIGHT_46_TM=57.768
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=944
PRIMER_PAIR_47_PENALTY=3.242399
PRIMER_LEFT_47_PENALTY=1.010529
PRIMER_RIGHT_47_PENALTY=2.231870
PRIMER_LEFT_47_SEQUENCE=CGCAAGTGAAAATATGTCGGT
PRIMER_RIGHT_47_SEQUENCE=GGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_47=801,21
PRIMER_RIGHT_47=1745,20
PRIMER_LEFT_47_TM=60.011
PRIMER_RIGHT_47_TM=57.768
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=945
PRIMER_PAIR_48_PENALTY=3.253808
PRIMER_LEFT_48_PENALTY=1.047652
PRIMER_RIGHT_48_PENALTY=2.206156
PRIMER_LEFT_48_SEQUENCE=GAAGGTATTGGCCAGGAAAAG
PRIMER_RIGHT_48_SEQUENCE=AGGTTTCGGATTCTTCCTCTC
PRIMER_LEFT_48=896,21
PRIMER_RIGHT_48=1746,21
PRIMER_LEFT_48_TM=59.952
PRIMER_RIGHT_48_TM=58.794
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=851
PRIMER_PAIR_49_PENALTY=3.257537
PRIMER_LEFT_49_PENALTY=0.691563
PRIMER_RIGHT_49_PENALTY=2.565974
PRIMER_LEFT_49_SEQUENCE=GGCTGAGGAAGCTGAAGAAG
PRIMER_RIGHT_49_SEQUENCE=AAATATACCATCGGTTCCAGCA
PRIMER_LEFT_49=927,20
PRIMER_RIGHT_49=1863,22
PRIMER_LEFT_49_TM=59.308
PRIMER_RIGHT_49_TM=60.566
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=937
=
SEQUENCE_ID=MAGIC.338_5_5665393_5665838_wu-0_no-0_21751_2958_TRUE
SEQUENCE_TARGET=950,545
SEQUENCE_TEMPLATE=TTTTATCCTTTGTTATTTTTAGTCTGGTCCGTNTTTTCTCCGTATCTTGTTTCAAATACATTNTCTCGTAAAGAAACCGCNTTTTTTGGTTTAGTACTGAATGATAAAAGTATTGTTTTCNTACGAAATTTANGTCAACCGNCCTAGTNCTAGAGGATCACCTAGACCATATGCATGGGACTTNGTCGAGCTTCGATGTGGATTAGCCCNAGTTATCAATAGAATACTGAAAATTGGCNNNNNTAACTTTGATTTAATGGAAATGCNTTTTTATANNATACTNAAAATTTAANAGANATGATGATNGATGAGTAGTAAATTTCAAATGGTATAACAAGTTTAATTGTTTTTTTAGTAAGTTATATATACTGANCAAGACGTTTTTGTTTGAGTCAGTGACTATCNCTCTTTGTTTTTACTCAACTTAATTCCATANATCCTGGTACTTATAACGTGTACAATCTAGAATCACAAGCGTTTAGAGATAGTGATAATTAGCTTGGCTACTTTCTCAGCTGTGTTGTTGCTTGATCGANGNNGGAAAATGANAGTGGNCATATTGAAGTTCTTGCTTAGNGCTATTGNTAATAGAACGGTTNGAGAGCCGGCCAAGCTTATGGATAATATATTTATANATGGAGAATGAGAATAACATAAAGATGAGANATNGTTGAACCTAATTAATNAAAAAAACAATTTACATGAANTTTTTTTCTTTANAAGATTTCANTCTGNAACATGCATAGAAACTTGTCTCATNACCCTCGTAGTCNTATAGCAAGCATTAGTTTTATGATAGAGATGTTTCNGTTACGAAAGAAANTTTAAACCAACTTAAGTGGTTGTAAATCACGTTGTANTTTTTATGTTTATGTACAANTNCAAATTACAAATCTTCNTTTTTAATTATAATCTTTGCCACTGAAATTCACGATAATATCACTAAGGTTCGATGCAATTCGTCAAACATNTCACGTACTAAAATTTGTTNGGCGANNGANGGAGACATAGATGTAAATGGAAACGTNNACTCGTAAATTGTGTCATCTTTCGTGGCCCATNAANTNGAGATGGAGTTTTATCGTTTTCTATATCNNNNNNNNNNNNNNNNNNNNNNAANAAGGTTTTATTATAAAAAGTACCAAAGAAATGGACCGTAGTCACCATNNNNNNNNTTACATGGTTACATGGCGCGACACGTAGTGTCACTTAATGCTGCAAGTGTTTTTTCCTCTTTGTTTTTTGCCTCCCTGAATATTTTAAATTTGNTATATAAAATNTTTCTGCTGACGAGTTTTAGTTACTCTAGTTTCTAAAATGGTCTAGAAAAAGTTGTGNTATATAANTATAATAACTCGGTAANGGCTACGTACAGAGTCAGCCACGTAACANTAAATGGATGTGTCATGGACCCGTATTACACTCNNCATTATACACGNNTNNNGNNTANATNNNNTANNNNNNGATNANNNANNTTTNNNNTNTCAANNNNGANANANNTANTNNNGTANNNNNTNTTCNAAANNCATAGTNANTNTANNTNATNANAAATANGTNTTNANTNGTTTNNAAACNANTNNNCANNNNNNNANTTATTNTTNTNNCNNNNANTNANTNTGANTNNTTTNNNANNNGNAANTANCTATNTNNNNNNTGNNNNNNNAANNNNNNTTNTTTNNTNAANANTANNTNTNATTNTNNANNNTACANNNANNCCACAACTAGAAAGACGANTCCAAAAACNAAAGNAAATAGGAANTACTCGACCTTTNTTTTCTAACNTCAATCCACCACATNATATTTGTNGTTTTTAGCGNAATAATTNAGTTCTAACACACCACATGATTTGCTAAGCTACCAGTACTACTTTCTTTGTTAGGCCNTGAGAAATTGAATATTCCTTCCTCTTATTCTGCATATTCTTTGTATTTGTTTTGTTACCTACCTCTTCTAGTGTGTAGCCTACGTAAATATACACTCAAGTTGTTTTGATGTAACCNGTTTTCTCCACCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACACTACATATCAATTCNTACTTATTATAANTACACTNATTANATNTNAATGCATGAANNNAAACTCGAATTCGATATNNTNCGATTCGTCAAAAAANNNNNNNNNNNNNNNNNNTGGTATCAATTAGAGGTCTGAATTTGTTCAATATCGTCCACAATTCCCTACCTAAAAAATTAGAGCTTTTTGGACCAAATCCAAAAATGGATCTAAAAAGTGAAAACCAAAATATTCCTTTAAGAATCTACACAGATTCGAACCAAACCGGATTAAACCGNATAGTCNACGGCACGTGTGGAAACGCGNNNCGTTTCAAAAACATTTGGGGTANTNNNNNNNNNNNNNNNNNNNNNNNNATCAGGGTTTTAGGAGAAACCCAGGAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8831732_8832461_sf-2_po-0_3734_94434_TRUE
SEQUENCE_TARGET=950,829
SEQUENCE_TEMPLATE=NCTTCNGCTTCNTTTTTCCTCCAAAGCTCGAGCATTGTTAAGTAACAATGAATGCTTTATTTGCAGGTTATAAATGTGTATAACCAAAAGTATGAGAGCGCAGGGAAGTTCTGGCCTGACGTTCACAGGCGTGTTGTGACCGCATTGGTTGTTTCACAGCTGCTCTTGATGGGTCTTCTAAGCACCAAACACGCTTCTAAGTCCACTCCTTTGNTNCTTGTGCTTCCGTTGCTGACCATCGGGTTCCACAAACACTGCAAAAACCGTTACCAACCNGCCTTTGTCACATACCCGTTACAGGTCAGTTAATACAAAAAACAGAAATAACTGAAGATCTCTAGTAGCTATACGTAAAGCGTGTAAGCTAACCTTTTTCTTGTTTCGAGCCAAAGCAGGAAGCCATGATCAAAGATACACTGGACCGTATACGTGAACCAAACTTAAACCTCAAAGCTTTCCTTCGAGACGCCTACGCCCACCCGGAGTTCAGGGTTGGAGAAGATCCAGAGCCCGAGGAGAAGCTGGAGTCTGATATGTCACCTCCGGATTTAGTGGCCACTAAGCGTTGGTCATGGAGGAACACTCCTTTGCCTAGCAAAGATAGCTGCNGTGNNATACCCTAANCCTTGCTCTGTTCTACCTTTANNGGTGATTCTTTTATATTCTTACACAAGTTTTCNTCTCATTAGAAGAAATGTACATATATACGATTGTATTATATGCAANANAAAAAAATACCATTTGGAAAAGCATACGAGAAGTTNCTGTTAGAANTGTNTCATATNATTACAAAAANCAGAAAANANAATACTCAGGTCATGACTTCTATAAATCCTCNGGTCAATGCCATCTAATGNNNNAAGTATGGTACTCNNNNNNNNNTGCATCCTGTNGCAATCATCCTTCTATGNANATCTAGNGCAATGCCCATTTCGTCAGTCACACAAANCATACAGATAACNAGATAAGTGTCCCATAGAGAGAAGAAACANCACTCTCTNAGANATTTTATCAAACACTTNNAAATCACTTTCCCTTGAGAACACAAACCAGTCACCAAAATGTCAACTTTTACAGATCAGATTCCATACCTCTTGNAATCTTAATAACATCACAAAGAACACAANAATTCCAGAATCAAATTGCTTCAACCGAGGTTATCAGTCATAATCGTCCTACAACAAATGAAAAGTTTTTACTTGATTATTANTATTNGTAACTTGTAAGCAAGCCCATATCANAATTTGATTNTGGGCCTCTGGCCTTTAAGAATATCCAAATATTATATCTTTAGTATTTTTGCATGGAGTTTTTGACGGATGGGNCAAAATACTGNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTGGGTTGTTGAAAAACTCNAACCAANGAAAATTTNAAAAANCTACAACCATNNNNNNNNNGCCTTACTTATAAGCTTTTCAGTTTTTATTGAAGTANACTAAATGTTTTGATTGTTAGAAGAGATAGAACATGTACATATTGGACAATTTACTATCCACCGTTAGAACATTGCGTGANCGACTCAGTCATAGTACTAGTCAGGTTTGTAGTTATAGACANATCAAGATTTCTGTCTAGTTCATGTCTATATCAAAGGTTGTGGTNTCTCTGCATTCGAAGACTTATGTTTCTTTTTTTGTCCGTAAATTTTTAAGATTTATCTTTTCTTGATAGATANATATNTATCACAATGCATAGCAATATCGACGTGATTGGCCCAGAATCAAGTCTTATCTTTGGTTGTGTTCAATAATGTAAAGTCCCTTTATAAGCTGGTACAATATGATGGTGCAGTGTTTGGAACTATATTATAAAAGGAATGATGACTCAGGGACCATGTAAAGCGTTAGTACAAATGTGTCGTTGAGACNTGAAACTTACTTGGGACCATAATTATGTTTCTTGGGTTTCTTTTTTTCAGTGCTAGTAAACTTTCTCATCAGTTTCTCTCTCGTTGATTANATTCTAAAAATTTCTCCATATATGCTCATAGTGAAAACGTAAATCATGTCNTCTGAGTCGTGAAAGCTGAAGTCGAAAGNNNNNNNNNNNNNNNNNNNNNNNGAAATGGGCTTTGCTTCAGGTTGCTGNAGGTGGAATCCTAGGATATTTACATATTTGGAGAGAAGCAAAANTTATAGCTTATAAGATTTTNGTCATTGTTATGATTTNTCAATTCTTAANTTGTNNGTATAATTTATATTTGTCTTCNGTTAANNNNNNNNNNNNNNNNNNNNNNNNNNNANCTCTTTCTCACATTGCTCTGAACTGAATCNCCT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_24177167_24177236_mt-0_no-0_2085_6247808_TRUE
SEQUENCE_TARGET=950,169
SEQUENCE_TEMPLATE=NNANNNNNNNNNGGNNNNNNGTGNNNANTTGNGNTGAATNNGNNTNNNNATNNNTTNNNNNNNNNNANNAGTNTTNANNNCTGTGGNNAANGANNNNTCNANNNTNCNNTTNNNNTNTGNNNNNNNTCNGNNNNTNNNNAGTTNNNNNNNNTTNNNTNNGGANNNNNNNNTTGNNCNNNTTAANNNANNTTGTTCNNGNTTNAANNNNTNNNNNANTNNNNTTCGNTNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNTNNNNNNNNNTNNNNNANNNNNNNNNNNNNTNNNNANCNANNNNNNNNNNNTNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNTNNNNAGNNNNNNNNNNNNNNTNNNNNTNNNNNTNTNNGNNNNANNNNNNNGNNNNNNNCNNNNNTNNNNGNNNNNNNNNNNTNNNNNCNCNNCNNCNCCNNTNNTCNNCNNTNNNCTNCTCNCNNNNNCNCCCNCNNCNTNNNNNNCTCNNNNTNNNNCTCNNNTNCNCNNNCNCNCNNNTNNCCNNNNNNNNCNNNNTNTCCNNNNNNNNNCTNTCNTCNNNNCNGNNNNNNCNNTTNNNNNNCNGANNTNNNNNNNNNNTNNCNNNNNNTCNTNNNNNNNNATNNNNCNNCNTNNNNTNNTNTNGNNGNNNTNNATNNNNTNNNNNGNNTNNNNNNNTNNNNNNNNNNNNNNTNNNNNANNNNTNNTGNNNNTNNTANNNNNGNTNTNNNNNTNNNNTTNNNNCNTNNACTNNNNNNNNNNNTNNANNNATTNNAGNNNNNTNCNNNNNTGNTANNNNANTNNNNNNNTNNNNTNNCTANNNNNTNNNTNNTTNNTNTNNNTNGNNNNNNNNNNNNTNANANNNNNNTNNTNNNNNTNNTNNNNNGTNNNNNTNTNNNNATGNNNNNCNNNNNNNNNNNNNNNNTNNNNNNNNNNNNTTGNNNNNNANNNNNNTNNNNNNGTNNNNNCNATNNNANTNGTTCTNNATNTTCNTNNANNGTGNNNATNANGTTNAAANCNANTNNNANTNTTCTNCNTTTCNTANANAGNGNCNANGCNNNTNNNNGNNNCTCCANNNTTNNNNGGNTCNNNTNNCCANNNTTNTNNNTNNNNNNAAGANANNNNNNCNATTGNGTGCNNNNNNNTTNTGTNNTTNTTNCNANNNTTNTNNNTCCNGGNNAANANCNTCNTNNNTNGANNNNTTNNTNNNTCTGGTNTGTNTNNNANNTTNTNTNAAANTCTNNNCANGNNNNNTNNTNCTTNGNTTTNNNNTTANANNTGANNNNNNTNNNGNNNNNTCNATNNNNTACATNNTNNTANNTNTGNTNNNTNNNTTNNATNANANTCNTTCNNNTNACTTNANNNTTNGNTTTNNTNATNNNTGNNNNNNNCNNNTTTTCTNTGTTGATNNNNNNNANAAANANGTNANTNGNTNGNNNTCANNCTNNCTTNNNNTNTGNNNNTTGNNANCTGCNGCTNNNATNNTTNTTCTTNTTGATNGNNANNATCNGGANNANGTCANNNNNTNNTCNCNNNNNCTTTNNTTNTGNNNGTNGAGNNNGCATANNNNNNNNNTNTANNNTNTGNNCNTCTNNNTTNNNANGNGGNAANNNNNNTTNNNNNNGATNAANTNANACNNNTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNTNGNCNAGTTNNCNTNTNTNGGNATNTNANTNNGCNNGTNNCNNNNNATNANTNATANNANNNTNCNTNNNTNCNNTNTNNNTNNANNANNNTNNNNTANTNCNNCGNTNNNNNNANTNTTATATTGAAGNTAGACCTTANTNGTANANNNNNNNNNNACACCTGTATACTATGAAAACTGGTGNGCCNNGAGCATATTTGACCTGANTNTNTCCTNTCATATTTCCNTGNTGTTGCNTCTGAGACTGANCTGCNTCNTATNTTTGCAGGGTGGTGNCTTATGGTTCCAGAANTTGAGTGATCTTCCTGGNGGNTCTTTTGGACCAGTCTTNCCAATTTTAATTGCTAC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_1799604_1799912_sf-2_ler-0_445081_3094_TRUE
SEQUENCE_TARGET=950,408
SEQUENCE_TEMPLATE=TTATAATACGTTATACCATTGACAGAAACATCGAAGACTCTGGATCCATCACCCATCGANTCAGAATCATGAGCAAAGTAAAGCGCAATATAATAAGTAGCCATTTTCAATGGCATTGGAGGCCATGTGAACTCCAAAGGCTGAACTTGAGTAGCTCTTAGATCGGTGTTAAATATCCTCGACGGCGGAAGGTTCCAGAAACCAGAAACCTCTAGTTTCCTATTGTTTGGTACTGTCGAATTTAACGAATAAGGCTCCCAAAACCTATCAAACTGATCATCTGGAAACCTAAACAAGAAGAATGTTGATAATGGTGAATACATNGCCTTGGTTTCGAAAGATTTAGCATCGAAGGAACAATTTTTCTGAGAAAGTATCAAACTTTNNATCAAAGTAGTGAACNTTTTTACCTGATGATTGGTCCACTGTAACCAAAAGCATGCCTAGCAACAAGACTCATGCCAACAGTGGTAAAGTCAGTGGAATTGTAAAGAGTACCATCAAGCCTAACGAGCTCCAAAGCTGAGATAAATGGATCAGAAGTTGTATAAGAATTAGAAGCAACGCAGACGCTTATAGATTTCCCCTGCGCCAGAAAAACCCCTTCGTAATAAGAAGCGAGACCATCTGCGTAATCGGCGGTNGTATTGACGATCCCCCATAACGTGCCGTCGACAATCTGATCAAATACCGGCGGNGGAGTACCTTTACCGTTAACTCCTCCGTAATAGTATGTCGTCCTGATCATATACTTCCAGCCACGAGAAACGCTCATCACGTAGCAAAACTTGTGATGAGTTCCGTCGAGGGAAAGAGGGAAAGATCTNACAGTGAATAAGATTTCGTCGAGGACTTGATCGGANACGTTTTTNGGTGTGCCGCTGGAGACGAAANTCTCGTCGGGTTGCCATTGTCGGCCGTCGATTACGGAGGAGGAAGAGGCGCCACAATCGATCAGAATNGCTAAATTNTAAAATTAGAAATGTNACAAAATTGGAAANTGGAAGGGATANGAATTTAGGCNATGGNNNNAAGAGATTGAGGTGATACCTGAAGGAGNAGAGGGCTGAGAGAGGGAAGAGGAGANGAGGGAGAAGACNGNGAGGAGGAGGAGGAGGAGNGGGGACATGGCGGCGGAGACGNNGGTGGNAGAGAAGAAGTTAGCATNAAAGAGAGGTTTAGGTTGGAAGAAGGAGGGGGGTGGGTTGACCCTCCAGCTCCAGAGAATTTGAAGAATGAGAGAATTAGAGGAACAGGTCAGAGAGGTAGCNNNNGNNGANNNNNTTNNNNNNNNNNNNTTNATTNNTTNTNNGANNNNNANANNTTNTTNTNTTNNTCNNTTNNTTNTNTTNNNGTCNANNNNGNNANNNNNNAATTATNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNTNNNNNNNAANNANNNNTNNNTNTNNAATNNNNNCCNNNNNNNNNNNNNNNNNNANNNTNNNNTTNANNNNNNNNNNNNNNNNNNNTNANNNNNNNNTTTANNTAATNNTNNTTNNNNANNNNNNTNNNNNATNNNGNTTNNNNNNNNNNNNNTANNNNTTNNNANNNNNNNTTNNNAANNNNNNNNNNATNNNNTNNAANNNNNCNATNNNCNNNNNNNNTTTTCANNNNTNANNNNTNNNNANNNTNNNNCNNNTCNNTNNNNTNATANNTNTNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACACCTATGGATCGAGATCAAACATCANACCCTACATAACCAANCCTGTTCTAATTAACATTANTAACTTTAATTANNATATCTTAAACTTCATANNATTTGGAGNCTCNTNTTAAGTAGTGTTTACGTTTNCTCACTTTTCTTTGCNTCTTCAATGAAAAATTGCATTAAAGCTTTCGGATGCNAAGCGTGCTCGTGGTGAATCACAAGANTTCATTTCATGTGCTCGCATTAATAAAATATAACATTGGTATCTNTTTGCATTTTNTTCAAAGCTCATACACGTTTACTTTGTGGCCNACCACAACGGTCCAAAAAGTCTCTAAAATATTACTTATTTTGTAAACTATCGTNAAAATACGGAAAACATCCANCTCAATGAATATTTAGGATGAGTAGAGCTGTACGTAATAAGAGGATGTTGATGCTAAGATAACGTTTATTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.556888
PRIMER_LEFT_0_PENALTY=0.182658
PRIMER_RIGHT_0_PENALTY=3.374230
PRIMER_LEFT_0_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_0_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_0=802,20
PRIMER_RIGHT_0=1795,21
PRIMER_LEFT_0_TM=60.183
PRIMER_RIGHT_0_TM=57.626
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=994
PRIMER_PAIR_1_PENALTY=3.607066
PRIMER_LEFT_1_PENALTY=0.232835
PRIMER_RIGHT_1_PENALTY=3.374230
PRIMER_LEFT_1_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_1_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_1=920,20
PRIMER_RIGHT_1=1795,21
PRIMER_LEFT_1_TM=59.767
PRIMER_RIGHT_1_TM=57.626
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=876
PRIMER_PAIR_2_PENALTY=3.653114
PRIMER_LEFT_2_PENALTY=0.182658
PRIMER_RIGHT_2_PENALTY=3.470456
PRIMER_LEFT_2_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_2_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_2=802,20
PRIMER_RIGHT_2=1795,22
PRIMER_LEFT_2_TM=60.183
PRIMER_RIGHT_2_TM=58.530
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=994
PRIMER_PAIR_3_PENALTY=3.653114
PRIMER_LEFT_3_PENALTY=0.182658
PRIMER_RIGHT_3_PENALTY=3.470456
PRIMER_LEFT_3_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_3_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_3=802,20
PRIMER_RIGHT_3=1796,22
PRIMER_LEFT_3_TM=60.183
PRIMER_RIGHT_3_TM=58.530
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=995
PRIMER_PAIR_4_PENALTY=3.703292
PRIMER_LEFT_4_PENALTY=0.232835
PRIMER_RIGHT_4_PENALTY=3.470456
PRIMER_LEFT_4_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_4_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_4=920,20
PRIMER_RIGHT_4=1795,22
PRIMER_LEFT_4_TM=59.767
PRIMER_RIGHT_4_TM=58.530
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=876
PRIMER_PAIR_5_PENALTY=3.703292
PRIMER_LEFT_5_PENALTY=0.232835
PRIMER_RIGHT_5_PENALTY=3.470456
PRIMER_LEFT_5_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_5_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_5=920,20
PRIMER_RIGHT_5=1796,22
PRIMER_LEFT_5_TM=59.767
PRIMER_RIGHT_5_TM=58.530
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=877
PRIMER_PAIR_6_PENALTY=3.816697
PRIMER_LEFT_6_PENALTY=0.182658
PRIMER_RIGHT_6_PENALTY=3.634039
PRIMER_LEFT_6_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_6_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_6=802,20
PRIMER_RIGHT_6=1796,23
PRIMER_LEFT_6_TM=60.183
PRIMER_RIGHT_6_TM=59.366
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=995
PRIMER_PAIR_7_PENALTY=3.866874
PRIMER_LEFT_7_PENALTY=0.232835
PRIMER_RIGHT_7_PENALTY=3.634039
PRIMER_LEFT_7_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_7_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_7=920,20
PRIMER_RIGHT_7=1796,23
PRIMER_LEFT_7_TM=59.767
PRIMER_RIGHT_7_TM=59.366
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=877
PRIMER_PAIR_8_PENALTY=4.122196
PRIMER_LEFT_8_PENALTY=0.182658
PRIMER_RIGHT_8_PENALTY=3.939538
PRIMER_LEFT_8_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_8_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_8=802,20
PRIMER_RIGHT_8=1794,21
PRIMER_LEFT_8_TM=60.183
PRIMER_RIGHT_8_TM=57.060
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=993
PRIMER_PAIR_9_PENALTY=4.142091
PRIMER_LEFT_9_PENALTY=0.182658
PRIMER_RIGHT_9_PENALTY=3.959434
PRIMER_LEFT_9_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_9_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_9=802,20
PRIMER_RIGHT_9=1794,20
PRIMER_LEFT_9_TM=60.183
PRIMER_RIGHT_9_TM=56.041
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=993
PRIMER_PAIR_10_PENALTY=4.172373
PRIMER_LEFT_10_PENALTY=0.232835
PRIMER_RIGHT_10_PENALTY=3.939538
PRIMER_LEFT_10_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_10_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_10=920,20
PRIMER_RIGHT_10=1794,21
PRIMER_LEFT_10_TM=59.767
PRIMER_RIGHT_10_TM=57.060
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=875
PRIMER_PAIR_11_PENALTY=4.192269
PRIMER_LEFT_11_PENALTY=0.232835
PRIMER_RIGHT_11_PENALTY=3.959434
PRIMER_LEFT_11_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_11_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_11=920,20
PRIMER_RIGHT_11=1794,20
PRIMER_LEFT_11_TM=59.767
PRIMER_RIGHT_11_TM=56.041
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=875
PRIMER_PAIR_12_PENALTY=4.414623
PRIMER_LEFT_12_PENALTY=0.182658
PRIMER_RIGHT_12_PENALTY=4.231965
PRIMER_LEFT_12_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_12_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_12=802,20
PRIMER_RIGHT_12=1797,23
PRIMER_LEFT_12_TM=60.183
PRIMER_RIGHT_12_TM=61.232
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=996
PRIMER_PAIR_13_PENALTY=4.464800
PRIMER_LEFT_13_PENALTY=0.232835
PRIMER_RIGHT_13_PENALTY=4.231965
PRIMER_LEFT_13_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_13_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_13=920,20
PRIMER_RIGHT_13=1797,23
PRIMER_LEFT_13_TM=59.767
PRIMER_RIGHT_13_TM=61.232
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=878
PRIMER_PAIR_14_PENALTY=4.490469
PRIMER_LEFT_14_PENALTY=1.116239
PRIMER_RIGHT_14_PENALTY=3.374230
PRIMER_LEFT_14_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_14_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_14=921,20
PRIMER_RIGHT_14=1795,21
PRIMER_LEFT_14_TM=58.884
PRIMER_RIGHT_14_TM=57.626
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=875
PRIMER_PAIR_15_PENALTY=4.573597
PRIMER_LEFT_15_PENALTY=1.199367
PRIMER_RIGHT_15_PENALTY=3.374230
PRIMER_LEFT_15_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_15_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_15=803,20
PRIMER_RIGHT_15=1795,21
PRIMER_LEFT_15_TM=61.199
PRIMER_RIGHT_15_TM=57.626
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=993
PRIMER_PAIR_16_PENALTY=4.586695
PRIMER_LEFT_16_PENALTY=1.116239
PRIMER_RIGHT_16_PENALTY=3.470456
PRIMER_LEFT_16_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_16_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_16=921,20
PRIMER_RIGHT_16=1795,22
PRIMER_LEFT_16_TM=58.884
PRIMER_RIGHT_16_TM=58.530
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=875
PRIMER_PAIR_17_PENALTY=4.586695
PRIMER_LEFT_17_PENALTY=1.116239
PRIMER_RIGHT_17_PENALTY=3.470456
PRIMER_LEFT_17_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_17_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_17=921,20
PRIMER_RIGHT_17=1796,22
PRIMER_LEFT_17_TM=58.884
PRIMER_RIGHT_17_TM=58.530
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=876
PRIMER_PAIR_18_PENALTY=4.633452
PRIMER_LEFT_18_PENALTY=1.259222
PRIMER_RIGHT_18_PENALTY=3.374230
PRIMER_LEFT_18_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_18_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_18=919,20
PRIMER_RIGHT_18=1795,21
PRIMER_LEFT_18_TM=58.741
PRIMER_RIGHT_18_TM=57.626
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=877
PRIMER_PAIR_19_PENALTY=4.669823
PRIMER_LEFT_19_PENALTY=1.199367
PRIMER_RIGHT_19_PENALTY=3.470456
PRIMER_LEFT_19_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_19_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_19=803,20
PRIMER_RIGHT_19=1795,22
PRIMER_LEFT_19_TM=61.199
PRIMER_RIGHT_19_TM=58.530
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=993
PRIMER_PAIR_20_PENALTY=4.669823
PRIMER_LEFT_20_PENALTY=1.199367
PRIMER_RIGHT_20_PENALTY=3.470456
PRIMER_LEFT_20_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_20_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_20=803,20
PRIMER_RIGHT_20=1796,22
PRIMER_LEFT_20_TM=61.199
PRIMER_RIGHT_20_TM=58.530
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=994
PRIMER_PAIR_21_PENALTY=4.729679
PRIMER_LEFT_21_PENALTY=1.259222
PRIMER_RIGHT_21_PENALTY=3.470456
PRIMER_LEFT_21_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_21_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_21=919,20
PRIMER_RIGHT_21=1795,22
PRIMER_LEFT_21_TM=58.741
PRIMER_RIGHT_21_TM=58.530
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=877
PRIMER_PAIR_22_PENALTY=4.729679
PRIMER_LEFT_22_PENALTY=1.259222
PRIMER_RIGHT_22_PENALTY=3.470456
PRIMER_LEFT_22_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_22_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_22=919,20
PRIMER_RIGHT_22=1796,22
PRIMER_LEFT_22_TM=58.741
PRIMER_RIGHT_22_TM=58.530
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=878
PRIMER_PAIR_23_PENALTY=4.750277
PRIMER_LEFT_23_PENALTY=1.116239
PRIMER_RIGHT_23_PENALTY=3.634039
PRIMER_LEFT_23_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_23_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_23=921,20
PRIMER_RIGHT_23=1796,23
PRIMER_LEFT_23_TM=58.884
PRIMER_RIGHT_23_TM=59.366
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=876
PRIMER_PAIR_24_PENALTY=4.768028
PRIMER_LEFT_24_PENALTY=0.182658
PRIMER_RIGHT_24_PENALTY=4.585370
PRIMER_LEFT_24_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_24_SEQUENCE=TGATCTCGATCCATAGGTGT
PRIMER_LEFT_24=802,20
PRIMER_RIGHT_24=1793,20
PRIMER_LEFT_24_TM=60.183
PRIMER_RIGHT_24_TM=55.415
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=992
PRIMER_PAIR_25_PENALTY=4.818205
PRIMER_LEFT_25_PENALTY=0.232835
PRIMER_RIGHT_25_PENALTY=4.585370
PRIMER_LEFT_25_SEQUENCE=TCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_25_SEQUENCE=TGATCTCGATCCATAGGTGT
PRIMER_LEFT_25=920,20
PRIMER_RIGHT_25=1793,20
PRIMER_LEFT_25_TM=59.767
PRIMER_RIGHT_25_TM=55.415
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=874
PRIMER_PAIR_26_PENALTY=4.833405
PRIMER_LEFT_26_PENALTY=1.199367
PRIMER_RIGHT_26_PENALTY=3.634039
PRIMER_LEFT_26_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_26_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_26=803,20
PRIMER_RIGHT_26=1796,23
PRIMER_LEFT_26_TM=61.199
PRIMER_RIGHT_26_TM=59.366
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=994
PRIMER_PAIR_27_PENALTY=4.893261
PRIMER_LEFT_27_PENALTY=1.259222
PRIMER_RIGHT_27_PENALTY=3.634039
PRIMER_LEFT_27_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_27_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_27=919,20
PRIMER_RIGHT_27=1796,23
PRIMER_LEFT_27_TM=58.741
PRIMER_RIGHT_27_TM=59.366
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=878
PRIMER_PAIR_28_PENALTY=4.958263
PRIMER_LEFT_28_PENALTY=1.584033
PRIMER_RIGHT_28_PENALTY=3.374230
PRIMER_LEFT_28_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_28_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_28=919,21
PRIMER_RIGHT_28=1795,21
PRIMER_LEFT_28_TM=60.584
PRIMER_RIGHT_28_TM=57.626
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=877
PRIMER_PAIR_29_PENALTY=5.054489
PRIMER_LEFT_29_PENALTY=1.584033
PRIMER_RIGHT_29_PENALTY=3.470456
PRIMER_LEFT_29_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_29_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_29=919,21
PRIMER_RIGHT_29=1795,22
PRIMER_LEFT_29_TM=60.584
PRIMER_RIGHT_29_TM=58.530
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=877
PRIMER_PAIR_30_PENALTY=5.054489
PRIMER_LEFT_30_PENALTY=1.584033
PRIMER_RIGHT_30_PENALTY=3.470456
PRIMER_LEFT_30_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_30_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_30=919,21
PRIMER_RIGHT_30=1796,22
PRIMER_LEFT_30_TM=60.584
PRIMER_RIGHT_30_TM=58.530
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=878
PRIMER_PAIR_31_PENALTY=5.055777
PRIMER_LEFT_31_PENALTY=1.116239
PRIMER_RIGHT_31_PENALTY=3.939538
PRIMER_LEFT_31_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_31_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_31=921,20
PRIMER_RIGHT_31=1794,21
PRIMER_LEFT_31_TM=58.884
PRIMER_RIGHT_31_TM=57.060
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=874
PRIMER_PAIR_32_PENALTY=5.075672
PRIMER_LEFT_32_PENALTY=1.116239
PRIMER_RIGHT_32_PENALTY=3.959434
PRIMER_LEFT_32_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_32_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_32=921,20
PRIMER_RIGHT_32=1794,20
PRIMER_LEFT_32_TM=58.884
PRIMER_RIGHT_32_TM=56.041
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=874
PRIMER_PAIR_33_PENALTY=5.082730
PRIMER_LEFT_33_PENALTY=1.708500
PRIMER_RIGHT_33_PENALTY=3.374230
PRIMER_LEFT_33_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_33_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_33=920,21
PRIMER_RIGHT_33=1795,21
PRIMER_LEFT_33_TM=60.708
PRIMER_RIGHT_33_TM=57.626
PRIMER_LEFT_33_GC_PERCENT=52.381
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=876
PRIMER_PAIR_34_PENALTY=5.138905
PRIMER_LEFT_34_PENALTY=1.199367
PRIMER_RIGHT_34_PENALTY=3.939538
PRIMER_LEFT_34_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_34_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_34=803,20
PRIMER_RIGHT_34=1794,21
PRIMER_LEFT_34_TM=61.199
PRIMER_RIGHT_34_TM=57.060
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=992
PRIMER_PAIR_35_PENALTY=5.158800
PRIMER_LEFT_35_PENALTY=1.199367
PRIMER_RIGHT_35_PENALTY=3.959434
PRIMER_LEFT_35_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_35_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_35=803,20
PRIMER_RIGHT_35=1794,20
PRIMER_LEFT_35_TM=61.199
PRIMER_RIGHT_35_TM=56.041
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=992
PRIMER_PAIR_36_PENALTY=5.178956
PRIMER_LEFT_36_PENALTY=1.708500
PRIMER_RIGHT_36_PENALTY=3.470456
PRIMER_LEFT_36_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_36_SEQUENCE=TTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_36=920,21
PRIMER_RIGHT_36=1795,22
PRIMER_LEFT_36_TM=60.708
PRIMER_RIGHT_36_TM=58.530
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=876
PRIMER_PAIR_37_PENALTY=5.178956
PRIMER_LEFT_37_PENALTY=1.708500
PRIMER_RIGHT_37_PENALTY=3.470456
PRIMER_LEFT_37_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_37_SEQUENCE=GTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_37=920,21
PRIMER_RIGHT_37=1796,22
PRIMER_LEFT_37_TM=60.708
PRIMER_RIGHT_37_TM=58.530
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=877
PRIMER_PAIR_38_PENALTY=5.198760
PRIMER_LEFT_38_PENALTY=1.259222
PRIMER_RIGHT_38_PENALTY=3.939538
PRIMER_LEFT_38_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_38_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_38=919,20
PRIMER_RIGHT_38=1794,21
PRIMER_LEFT_38_TM=58.741
PRIMER_RIGHT_38_TM=57.060
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=876
PRIMER_PAIR_39_PENALTY=5.218072
PRIMER_LEFT_39_PENALTY=1.584033
PRIMER_RIGHT_39_PENALTY=3.634039
PRIMER_LEFT_39_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_39_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_39=919,21
PRIMER_RIGHT_39=1796,23
PRIMER_LEFT_39_TM=60.584
PRIMER_RIGHT_39_TM=59.366
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=43.478
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=878
PRIMER_PAIR_40_PENALTY=5.218656
PRIMER_LEFT_40_PENALTY=1.259222
PRIMER_RIGHT_40_PENALTY=3.959434
PRIMER_LEFT_40_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_40_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_40=919,20
PRIMER_RIGHT_40=1794,20
PRIMER_LEFT_40_TM=58.741
PRIMER_RIGHT_40_TM=56.041
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=876
PRIMER_PAIR_41_PENALTY=5.342539
PRIMER_LEFT_41_PENALTY=1.708500
PRIMER_RIGHT_41_PENALTY=3.634039
PRIMER_LEFT_41_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_41_SEQUENCE=GTTTGATCTCGATCCATAGGTGT
PRIMER_LEFT_41=920,21
PRIMER_RIGHT_41=1796,23
PRIMER_LEFT_41_TM=60.708
PRIMER_RIGHT_41_TM=59.366
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=43.478
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=877
PRIMER_PAIR_42_PENALTY=5.348203
PRIMER_LEFT_42_PENALTY=1.116239
PRIMER_RIGHT_42_PENALTY=4.231965
PRIMER_LEFT_42_SEQUENCE=CGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_42_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_42=921,20
PRIMER_RIGHT_42=1797,23
PRIMER_LEFT_42_TM=58.884
PRIMER_RIGHT_42_TM=61.232
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=877
PRIMER_PAIR_43_PENALTY=5.431331
PRIMER_LEFT_43_PENALTY=1.199367
PRIMER_RIGHT_43_PENALTY=4.231965
PRIMER_LEFT_43_SEQUENCE=TCGAGGGAAAGAGGGAAAGA
PRIMER_RIGHT_43_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_43=803,20
PRIMER_RIGHT_43=1797,23
PRIMER_LEFT_43_TM=61.199
PRIMER_RIGHT_43_TM=61.232
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=43.478
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=995
PRIMER_PAIR_44_PENALTY=5.491187
PRIMER_LEFT_44_PENALTY=1.259222
PRIMER_RIGHT_44_PENALTY=4.231965
PRIMER_LEFT_44_SEQUENCE=GTCGATTACGGAGGAGGAAG
PRIMER_RIGHT_44_SEQUENCE=TGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_44=919,20
PRIMER_RIGHT_44=1797,23
PRIMER_LEFT_44_TM=58.741
PRIMER_RIGHT_44_TM=61.232
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=43.478
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=879
PRIMER_PAIR_45_PENALTY=5.523571
PRIMER_LEFT_45_PENALTY=1.584033
PRIMER_RIGHT_45_PENALTY=3.939538
PRIMER_LEFT_45_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_45_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_45=919,21
PRIMER_RIGHT_45=1794,21
PRIMER_LEFT_45_TM=60.584
PRIMER_RIGHT_45_TM=57.060
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=876
PRIMER_PAIR_46_PENALTY=5.543466
PRIMER_LEFT_46_PENALTY=1.584033
PRIMER_RIGHT_46_PENALTY=3.959434
PRIMER_LEFT_46_SEQUENCE=GTCGATTACGGAGGAGGAAGA
PRIMER_RIGHT_46_SEQUENCE=TTGATCTCGATCCATAGGTG
PRIMER_LEFT_46=919,21
PRIMER_RIGHT_46=1794,20
PRIMER_LEFT_46_TM=60.584
PRIMER_RIGHT_46_TM=56.041
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=876
PRIMER_PAIR_47_PENALTY=5.576515
PRIMER_LEFT_47_PENALTY=2.202284
PRIMER_RIGHT_47_PENALTY=3.374230
PRIMER_LEFT_47_SEQUENCE=GATTACGGAGGAGGAAGAGG
PRIMER_RIGHT_47_SEQUENCE=TTTGATCTCGATCCATAGGTG
PRIMER_LEFT_47=922,20
PRIMER_RIGHT_47=1795,21
PRIMER_LEFT_47_TM=57.798
PRIMER_RIGHT_47_TM=57.626
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=874
PRIMER_PAIR_48_PENALTY=5.605008
PRIMER_LEFT_48_PENALTY=0.182658
PRIMER_RIGHT_48_PENALTY=5.422350
PRIMER_LEFT_48_SEQUENCE=GTCGAGGGAAAGAGGGAAAG
PRIMER_RIGHT_48_SEQUENCE=ATGTTTGATCTCGATCCATAGGTG
PRIMER_LEFT_48=802,20
PRIMER_RIGHT_48=1798,24
PRIMER_LEFT_48_TM=60.183
PRIMER_RIGHT_48_TM=61.422
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=41.667
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=997
PRIMER_PAIR_49_PENALTY=5.648038
PRIMER_LEFT_49_PENALTY=1.708500
PRIMER_RIGHT_49_PENALTY=3.939538
PRIMER_LEFT_49_SEQUENCE=TCGATTACGGAGGAGGAAGAG
PRIMER_RIGHT_49_SEQUENCE=TTGATCTCGATCCATAGGTGT
PRIMER_LEFT_49=920,21
PRIMER_RIGHT_49=1794,21
PRIMER_LEFT_49_TM=60.708
PRIMER_RIGHT_49_TM=57.060
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=875
=
SEQUENCE_ID=MAGIC.433_1_12349165_12349197_hi-0_ct-1_927_39811_TRUE
SEQUENCE_TARGET=950,132
SEQUENCE_TEMPLATE=AGCAGTGCANCATTCTNTGATCATTNNNNNNNNNNNNNNNNNNNCTNANACTTTGCCTTCTTAANGNCAANTANAGAACCANTGCNNAGACTTTGTGNTTCTANCNCGGAACANTTTCTATGTTCCTCGTTTGAGCATGNNCTTGGACTTTTGGCNTGTTNTGAGTCNNNTTTTGCTTTTNTTTCANTTTCATTTNNGTTNTTTNNNTAANTTGATTNNNCCANTCGTAGCCTAATCTTTGTTTGNTTTGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGNTNTNTNAGNTGACANGTTTTTATATNANTTAATANAGAGTCNAGAAAGAGNNNTTNTTTTCTCNATNAAAAGTTTCATNGTGTTGAGTTATGGNGCATAATCTCTNAAGACACACTCGACGNTNTCANTAAGGTATTTCACGTTTTAGTCTATNNTTGTTAANCCTAGNNTTTGTANACNTGATTTGANNNNNNNNNNNNNNNNNCTCATTNTAANAATGANNAGGCGNAAAGNGNNNAGATTTGNAANTTNTNANTANTACAAGAAAATGTTNGATTTNNNTANCGNGTANANNNTATNATAATNGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGTAANAANGTTNCCGACNAATTTCNTANCNTTCTNTTTGNANNGAAAANNGTTNCTCNCATTTTCNTNCCAANAATTATNTTTTTAATTAAANAAAGCTGCAATAGCNAAGTTNNNNNTTANCGTTTTACNTNTTTCNTTANNTCTCTTTTATNNNNNNNNNNNNNTTNNNNTTGCATTTANTNGTTAATTTGTGACTTNTTGAACTTTANAANTNCAANGNANTTATTTGTGGTATTTTNNTTGCNTTTTGGAGNCNTTTCATTNNCATTTGNCAANANAGNCNGATNNCNNACACNAGAGGATAAANNGAGNCANGAATGAGNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANTTGACCAGNATCANAAGATNGNNAATATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATATGACNNNNNNNNNNNNNNNNNAAAGAGNTTTCTAACTGTCACCAANCAAAACGTCATGTTTACACNAAAAAANTTGGACGTATTGNATCANTGATTTNGNTGCACCTAANATAAGGNGTNTNATTTAAGCAANNTTNNNNNNNNNNNNNNNNNNNNANAAAACCATNTTNTNAAAANNNNNNNNNAAANNNNNNNNNNNNNNNNNAAAACNTGACATGAATNATCTTATCAGNTTTCNCTNGAGCTACAACATCTCAANATATCATAGATTGATGCACTTTCCNAGTTTNTTCTGNAGTTCCTTCGTCTCTTTNTCCGCCCTCTCCAGATTCTCTCTGAGNTTCTTAATCTCATCGCTNGACCGCTTCTGAACTTCGTTTGCCCTCTTCTCCGCTTCAAGTCTTGCAGCTTGCTCTTCACCTAGTTGCTGCTCNAGTCTCTTAGCAGTGTCTCTCAGCTTAGTCTCCACCTNAACAACAACAANNNNNNNNNNNNNNNNTCAATGAAACTCTCCTGTGAGACCATAACTATTGATAAATNAAATTACCATCTCGGTGATGCGGCTAAGCTGCCTNTCATAGGACATATCNATTTGCTTCTTGAACTCATCTATCTCATTATTAGAGTAACCCTGAAGTAANTCAACCTCCTTTTTCTGGTCCCTTAGCTTGATAGCCTCTTCCTGAAANTGCAACCACANTTANAATCCTCNNCTNNAAAACAGANTAACTCANAAAAGTGAGTGAAAGNAACAATCTCCACCTGTAGCTCATGGAACAACTCATCTGAATACGGNTTTCCATTGTTCTGTTTNACAACAGATTCAACAAGNGAGAGAAGCTTCT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_5373046_5373192_can-0_edi-0_87151_122739_TRUE
SEQUENCE_TARGET=950,246
SEQUENCE_TEMPLATE=TTCGAAAGACGAATCCCCCNAAACCCAGGTGGCACGAGATGTGCACGTGCGAGAATATACGAGAGAGGCTCCCGAGAAAGAGATTAAAGNAAAAAAGGAAAGTTTATAAATATAAATACCNGTTGAATTTTGACTTTGAAGAAGGAGAGAGACAAAGCAGNNNNNNNNNNNNNNNNNNNNNNGAAGAAGAAGAAGAAGACTTTGGAGNAGAGAGAGAGAGAGACGTGGGGGCTATTTATGGCGATAATTTCTGATGCTGTCTCGTTTTCGGAGTTTCCNNTNTTTTTTTTTGGATTTATTGCTTTTTATTTTAATTTATGAGTTTTTATGATTTAATTAATTTGTTTTTGTGTGGTCGTTGGGNTTCTNTTTTTTTTAATAGAAGCGTGTATTTTGTAGATAGGAATTTACTCAAAAAGCTTAATCTCCCCACTATAATAAGTAACCTTCGTTTTAATTANTTTTTCTAAAATTTATTTTAATTTAATGTTTTCGTATTTTNTTTTAATTTAAGTGACGTATTGATGTTGTTGCGAATCTAGTACANCACTGCTGTCTAATTTAATGCTCTCTCGTTAGGTTTTGAACGTAGCTGGGGANNTTTTTTTGNTTTTNAAACTATACATTAANGTAGANTATCTCATAATTGGTTTATTTCTGTTTCTGTATAGAANTTAGTGCCATTATATTANTTTTATNACATCAACTAACCGTCAATTAATATTNNAAATANNTTTTNTNNNAATTAATTNTTTAAGTAATGGATTTTGAAGTTTTTAATTTAGAGAAGAATAGCAGTAAATCAGATGTGATATTTTTATTTCCAGTTATGTAGTACGCAAATTAATTAAATTTTATTTTCTTNATCTTTTNTTGTCNAAAAAACAAATTAAAGGTTTTNTGGATNATTTACTTTTGGAAAAATATAAATCTNATGTGGTAACAAATAAGTGAATTTTACAAATGTGTATTCTTNCNTTTTTGTNANAGTAATNCACAGNAAAAAGTGGAGTTTTATTCTATAATACGGNTTGTAACGCNTTTCCGTGAATATATTATGNTAATTTNTTAGGAATTTAANANNNATGGCCTATTCACATGTTTNGTTTTGTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTGTACTTNNNTTTTTTNNCCATGGATGTTTTGGCTANTAATTACTATGCNACTTCAACANNNNNNCCACNTTATGNTTCTAGTAGGAGTCATATCAAATCTTCTTANAAGTATTCCTACACCAGTATTGCTTACNTTGGTTTGGGCTTTTANGTCTANATTATNGGGNGAATTGACTGGCCCAATTGCTAAACATGATTGGGAATCANTTTCGTCCCAANCATNTTTTCCTTGAANAATCACTTTGATGACAATGACACACCCANANANTTATCANNGACTGCAATTAATCAGCAAATGGTTCCATAAGCCCATCTGCCCATGTTGGTGAAGAAGCATCACCTCAAATATATAATTTTAAGATGAGAGAGCAAACATTCCTTTCTGATCGCTCTGAATATTTTAGCAATTTACTAAACGTAAAGTNANNTTCATAGCTTTAATTCAAAATTTCTGTAANTAGANCTAGCAAAGAAACAAAGACNTGTGTTTAATATTATATTTTTGAAGAAGATAGTAGAANCTTCTGTCTNTTTGTTTAATATTTGAAACATCGAATAAATGGTTTCAATGAGTTGTCCTGANTCATCAGCCGACCCTTAACAATGACNACAACAACTTCAAATTTTAAAACATCAAGTATCTTCAACAACNTACCCTTTCCGTTTTATATTAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTATAATACANTANAGAAGAGTAAANNAAAAACTTTAAAACGACACATATAATAAAAAGGAAAAATAAATAAATAGATATGAGAAAAAAATACAGTTGGAACTTAATATATACACTTTTATACAACGGATACTAACGTTCAAAAATTTTATTCTTCTTTTGGTTAAAGTTTTATTTATTGTTTATGACTTTTCCATATCAGCTTCTTTTTTAATACNNTATNTATATNTTTAAAATTTGTTTATTTTATGCGAGCNAAAAAAAAAAAAAGTGTGCAAAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.147409
PRIMER_LEFT_0_PENALTY=0.110392
PRIMER_RIGHT_0_PENALTY=1.037017
PRIMER_LEFT_0_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_0_SEQUENCE=CAGAGCGATCAGAAAGGAATG
PRIMER_LEFT_0=579,20
PRIMER_RIGHT_0=1543,21
PRIMER_LEFT_0_TM=60.110
PRIMER_RIGHT_0_TM=59.963
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=965
PRIMER_PAIR_1_PENALTY=1.198893
PRIMER_LEFT_1_PENALTY=0.110392
PRIMER_RIGHT_1_PENALTY=1.088501
PRIMER_LEFT_1_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_1_SEQUENCE=GCTTATGGAACCATTTGCTGA
PRIMER_LEFT_1=579,20
PRIMER_RIGHT_1=1458,21
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=880
PRIMER_PAIR_2_PENALTY=1.411217
PRIMER_LEFT_2_PENALTY=0.110392
PRIMER_RIGHT_2_PENALTY=1.300825
PRIMER_LEFT_2_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_2_SEQUENCE=GCGATCAGAAAGGAATGTTTG
PRIMER_LEFT_2=579,20
PRIMER_RIGHT_2=1539,21
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.699
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=961
PRIMER_PAIR_3_PENALTY=1.440426
PRIMER_LEFT_3_PENALTY=0.110392
PRIMER_RIGHT_3_PENALTY=1.330034
PRIMER_LEFT_3_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_3_SEQUENCE=TCAGAGCGATCAGAAAGGAA
PRIMER_LEFT_3=579,20
PRIMER_RIGHT_3=1544,20
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=58.670
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=966
PRIMER_PAIR_4_PENALTY=1.632643
PRIMER_LEFT_4_PENALTY=0.110392
PRIMER_RIGHT_4_PENALTY=1.522251
PRIMER_LEFT_4_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_4_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_4=579,20
PRIMER_RIGHT_4=1339,20
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=61.522
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=761
PRIMER_PAIR_5_PENALTY=1.632643
PRIMER_LEFT_5_PENALTY=0.110392
PRIMER_RIGHT_5_PENALTY=1.522251
PRIMER_LEFT_5_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_5_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_5=579,20
PRIMER_RIGHT_5=1340,20
PRIMER_LEFT_5_TM=60.110
PRIMER_RIGHT_5_TM=61.522
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=10.9000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=762
PRIMER_PAIR_6_PENALTY=1.847851
PRIMER_LEFT_6_PENALTY=0.110392
PRIMER_RIGHT_6_PENALTY=1.737459
PRIMER_LEFT_6_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_6_SEQUENCE=GGCAGATGGGCTTATGGAAC
PRIMER_LEFT_6=579,20
PRIMER_RIGHT_6=1467,20
PRIMER_LEFT_6_TM=60.110
PRIMER_RIGHT_6_TM=61.737
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=10.9000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=889
PRIMER_PAIR_7_PENALTY=1.869475
PRIMER_LEFT_7_PENALTY=0.110392
PRIMER_RIGHT_7_PENALTY=1.759083
PRIMER_LEFT_7_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_7_SEQUENCE=GCTTATGGAACCATTTGCTG
PRIMER_LEFT_7=579,20
PRIMER_RIGHT_7=1458,20
PRIMER_LEFT_7_TM=60.110
PRIMER_RIGHT_7_TM=58.241
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=10.9000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=880
PRIMER_PAIR_8_PENALTY=1.962397
PRIMER_LEFT_8_PENALTY=0.440146
PRIMER_RIGHT_8_PENALTY=1.522251
PRIMER_LEFT_8_SEQUENCE=TGTTTTTGTGTGGTCGTTGG
PRIMER_RIGHT_8_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_8=342,20
PRIMER_RIGHT_8=1339,20
PRIMER_LEFT_8_TM=60.440
PRIMER_RIGHT_8_TM=61.522
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=998
PRIMER_PAIR_9_PENALTY=1.962397
PRIMER_LEFT_9_PENALTY=0.440146
PRIMER_RIGHT_9_PENALTY=1.522251
PRIMER_LEFT_9_SEQUENCE=TGTTTTTGTGTGGTCGTTGG
PRIMER_RIGHT_9_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_9=342,20
PRIMER_RIGHT_9=1340,20
PRIMER_LEFT_9_TM=60.440
PRIMER_RIGHT_9_TM=61.522
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=999
PRIMER_PAIR_10_PENALTY=2.379545
PRIMER_LEFT_10_PENALTY=0.110392
PRIMER_RIGHT_10_PENALTY=2.269153
PRIMER_LEFT_10_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_10_SEQUENCE=GAGCGATCAGAAAGGAATGTTT
PRIMER_LEFT_10=579,20
PRIMER_RIGHT_10=1541,22
PRIMER_LEFT_10_TM=60.110
PRIMER_RIGHT_10_TM=59.731
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=10.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=963
PRIMER_PAIR_11_PENALTY=2.722562
PRIMER_LEFT_11_PENALTY=0.110392
PRIMER_RIGHT_11_PENALTY=2.612170
PRIMER_LEFT_11_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_11_SEQUENCE=AGCGATCAGAAAGGAATGTTTG
PRIMER_LEFT_11=579,20
PRIMER_RIGHT_11=1540,22
PRIMER_LEFT_11_TM=60.110
PRIMER_RIGHT_11_TM=60.612
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.9000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=962
PRIMER_PAIR_12_PENALTY=2.745990
PRIMER_LEFT_12_PENALTY=1.223739
PRIMER_RIGHT_12_PENALTY=1.522251
PRIMER_LEFT_12_SEQUENCE=GTTTTTGTGTGGTCGTTGGG
PRIMER_RIGHT_12_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_12=343,20
PRIMER_RIGHT_12=1339,20
PRIMER_LEFT_12_TM=61.224
PRIMER_RIGHT_12_TM=61.522
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=10.0000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=997
PRIMER_PAIR_13_PENALTY=2.745990
PRIMER_LEFT_13_PENALTY=1.223739
PRIMER_RIGHT_13_PENALTY=1.522251
PRIMER_LEFT_13_SEQUENCE=GTTTTTGTGTGGTCGTTGGG
PRIMER_RIGHT_13_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_13=343,20
PRIMER_RIGHT_13=1340,20
PRIMER_LEFT_13_TM=61.224
PRIMER_RIGHT_13_TM=61.522
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=998
PRIMER_PAIR_14_PENALTY=2.804014
PRIMER_LEFT_14_PENALTY=1.766996
PRIMER_RIGHT_14_PENALTY=1.037017
PRIMER_LEFT_14_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_14_SEQUENCE=CAGAGCGATCAGAAAGGAATG
PRIMER_LEFT_14=578,20
PRIMER_RIGHT_14=1543,21
PRIMER_LEFT_14_TM=61.767
PRIMER_RIGHT_14_TM=59.963
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.2000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=966
PRIMER_PAIR_15_PENALTY=2.855497
PRIMER_LEFT_15_PENALTY=1.766996
PRIMER_RIGHT_15_PENALTY=1.088501
PRIMER_LEFT_15_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_15_SEQUENCE=GCTTATGGAACCATTTGCTGA
PRIMER_LEFT_15=578,20
PRIMER_RIGHT_15=1458,21
PRIMER_LEFT_15_TM=61.767
PRIMER_RIGHT_15_TM=60.089
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=11.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=881
PRIMER_PAIR_16_PENALTY=2.873086
PRIMER_LEFT_16_PENALTY=0.110392
PRIMER_RIGHT_16_PENALTY=2.762694
PRIMER_LEFT_16_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_16_SEQUENCE=CAGAGCGATCAGAAAGGAATGT
PRIMER_LEFT_16=579,20
PRIMER_RIGHT_16=1543,22
PRIMER_LEFT_16_TM=60.110
PRIMER_RIGHT_16_TM=60.763
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=10.9000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=965
PRIMER_PAIR_17_PENALTY=2.907645
PRIMER_LEFT_17_PENALTY=0.110392
PRIMER_RIGHT_17_PENALTY=2.797253
PRIMER_LEFT_17_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_17_SEQUENCE=GTGATGCTTCTTCACCAACA
PRIMER_LEFT_17=579,20
PRIMER_RIGHT_17=1489,20
PRIMER_LEFT_17_TM=60.110
PRIMER_RIGHT_17_TM=57.203
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=10.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=911
PRIMER_PAIR_18_PENALTY=3.032329
PRIMER_LEFT_18_PENALTY=0.110392
PRIMER_RIGHT_18_PENALTY=2.921937
PRIMER_LEFT_18_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_18_SEQUENCE=AGAGCGATCAGAAAGGAATG
PRIMER_LEFT_18=579,20
PRIMER_RIGHT_18=1542,20
PRIMER_LEFT_18_TM=60.110
PRIMER_RIGHT_18_TM=57.078
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=10.9000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=964
PRIMER_PAIR_19_PENALTY=3.067821
PRIMER_LEFT_19_PENALTY=1.766996
PRIMER_RIGHT_19_PENALTY=1.300825
PRIMER_LEFT_19_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_19_SEQUENCE=GCGATCAGAAAGGAATGTTTG
PRIMER_LEFT_19=578,20
PRIMER_RIGHT_19=1539,21
PRIMER_LEFT_19_TM=61.767
PRIMER_RIGHT_19_TM=59.699
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=11.2000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=962
PRIMER_PAIR_20_PENALTY=3.096255
PRIMER_LEFT_20_PENALTY=0.110392
PRIMER_RIGHT_20_PENALTY=2.985863
PRIMER_LEFT_20_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_20_SEQUENCE=AGAGCGATCAGAAAGGAATGT
PRIMER_LEFT_20=579,20
PRIMER_RIGHT_20=1542,21
PRIMER_LEFT_20_TM=60.110
PRIMER_RIGHT_20_TM=58.014
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=10.9000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=964
PRIMER_PAIR_21_PENALTY=3.097030
PRIMER_LEFT_21_PENALTY=1.766996
PRIMER_RIGHT_21_PENALTY=1.330034
PRIMER_LEFT_21_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_21_SEQUENCE=TCAGAGCGATCAGAAAGGAA
PRIMER_LEFT_21=578,20
PRIMER_RIGHT_21=1544,20
PRIMER_LEFT_21_TM=61.767
PRIMER_RIGHT_21_TM=58.670
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=11.2000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=967
PRIMER_PAIR_22_PENALTY=3.195357
PRIMER_LEFT_22_PENALTY=0.110392
PRIMER_RIGHT_22_PENALTY=3.084965
PRIMER_LEFT_22_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_22_SEQUENCE=GAGCGATCAGAAAGGAATGT
PRIMER_LEFT_22=579,20
PRIMER_RIGHT_22=1541,20
PRIMER_LEFT_22_TM=60.110
PRIMER_RIGHT_22_TM=56.915
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=10.9000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=963
PRIMER_PAIR_23_PENALTY=3.236252
PRIMER_LEFT_23_PENALTY=0.110392
PRIMER_RIGHT_23_PENALTY=3.125860
PRIMER_LEFT_23_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_23_SEQUENCE=GCGATCAGAAAGGAATGTTT
PRIMER_LEFT_23=579,20
PRIMER_RIGHT_23=1539,20
PRIMER_LEFT_23_TM=60.110
PRIMER_RIGHT_23_TM=56.874
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=10.9000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=961
PRIMER_PAIR_24_PENALTY=3.289247
PRIMER_LEFT_24_PENALTY=1.766996
PRIMER_RIGHT_24_PENALTY=1.522251
PRIMER_LEFT_24_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_24_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_24=578,20
PRIMER_RIGHT_24=1339,20
PRIMER_LEFT_24_TM=61.767
PRIMER_RIGHT_24_TM=61.522
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=11.2000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=762
PRIMER_PAIR_25_PENALTY=3.289247
PRIMER_LEFT_25_PENALTY=1.766996
PRIMER_RIGHT_25_PENALTY=1.522251
PRIMER_LEFT_25_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_25_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_25=578,20
PRIMER_RIGHT_25=1340,20
PRIMER_LEFT_25_TM=61.767
PRIMER_RIGHT_25_TM=61.522
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=11.2000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=763
PRIMER_PAIR_26_PENALTY=3.367033
PRIMER_LEFT_26_PENALTY=0.110392
PRIMER_RIGHT_26_PENALTY=3.256641
PRIMER_LEFT_26_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_26_SEQUENCE=GTTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_26=579,20
PRIMER_RIGHT_26=1341,21
PRIMER_LEFT_26_TM=60.110
PRIMER_RIGHT_26_TM=62.257
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=10.9000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=763
PRIMER_PAIR_27_PENALTY=3.464667
PRIMER_LEFT_27_PENALTY=1.942416
PRIMER_RIGHT_27_PENALTY=1.522251
PRIMER_LEFT_27_SEQUENCE=TTGTTTTTGTGTGGTCGTTG
PRIMER_RIGHT_27_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_27=341,20
PRIMER_RIGHT_27=1339,20
PRIMER_LEFT_27_TM=58.058
PRIMER_RIGHT_27_TM=61.522
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.7000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=999
PRIMER_PAIR_28_PENALTY=3.464667
PRIMER_LEFT_28_PENALTY=1.942416
PRIMER_RIGHT_28_PENALTY=1.522251
PRIMER_LEFT_28_SEQUENCE=TTGTTTTTGTGTGGTCGTTG
PRIMER_RIGHT_28_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_28=341,20
PRIMER_RIGHT_28=1340,20
PRIMER_LEFT_28_TM=58.058
PRIMER_RIGHT_28_TM=61.522
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.7000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=1000
PRIMER_PAIR_29_PENALTY=3.504455
PRIMER_LEFT_29_PENALTY=1.766996
PRIMER_RIGHT_29_PENALTY=1.737459
PRIMER_LEFT_29_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_29_SEQUENCE=GGCAGATGGGCTTATGGAAC
PRIMER_LEFT_29=578,20
PRIMER_RIGHT_29=1467,20
PRIMER_LEFT_29_TM=61.767
PRIMER_RIGHT_29_TM=61.737
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=11.2000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=890
PRIMER_PAIR_30_PENALTY=3.526080
PRIMER_LEFT_30_PENALTY=1.766996
PRIMER_RIGHT_30_PENALTY=1.759083
PRIMER_LEFT_30_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_30_SEQUENCE=GCTTATGGAACCATTTGCTG
PRIMER_LEFT_30=578,20
PRIMER_RIGHT_30=1458,20
PRIMER_LEFT_30_TM=61.767
PRIMER_RIGHT_30_TM=58.241
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=11.2000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=881
PRIMER_PAIR_31_PENALTY=3.531562
PRIMER_LEFT_31_PENALTY=0.110392
PRIMER_RIGHT_31_PENALTY=3.421170
PRIMER_LEFT_31_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_31_SEQUENCE=GAAAGGAATGTTTGCTCTCTCA
PRIMER_LEFT_31=579,20
PRIMER_RIGHT_31=1532,22
PRIMER_LEFT_31_TM=60.110
PRIMER_RIGHT_31_TM=58.579
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=10.9000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=954
PRIMER_PAIR_32_PENALTY=3.696786
PRIMER_LEFT_32_PENALTY=0.440146
PRIMER_RIGHT_32_PENALTY=3.256641
PRIMER_LEFT_32_SEQUENCE=TGTTTTTGTGTGGTCGTTGG
PRIMER_RIGHT_32_SEQUENCE=GTTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_32=342,20
PRIMER_RIGHT_32=1341,21
PRIMER_LEFT_32_TM=60.440
PRIMER_RIGHT_32_TM=62.257
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=1000
PRIMER_PAIR_33_PENALTY=3.790652
PRIMER_LEFT_33_PENALTY=2.268401
PRIMER_RIGHT_33_PENALTY=1.522251
PRIMER_LEFT_33_SEQUENCE=CTCAAAAAGCTTAATCTCCCCA
PRIMER_RIGHT_33_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_33=410,22
PRIMER_RIGHT_33=1339,20
PRIMER_LEFT_33_TM=59.732
PRIMER_RIGHT_33_TM=61.522
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=11.2000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=930
PRIMER_PAIR_34_PENALTY=3.790652
PRIMER_LEFT_34_PENALTY=2.268401
PRIMER_RIGHT_34_PENALTY=1.522251
PRIMER_LEFT_34_SEQUENCE=CTCAAAAAGCTTAATCTCCCCA
PRIMER_RIGHT_34_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_34=410,22
PRIMER_RIGHT_34=1340,20
PRIMER_LEFT_34_TM=59.732
PRIMER_RIGHT_34_TM=61.522
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=11.2000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=931
PRIMER_PAIR_35_PENALTY=3.814546
PRIMER_LEFT_35_PENALTY=0.110392
PRIMER_RIGHT_35_PENALTY=3.704154
PRIMER_LEFT_35_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_35_SEQUENCE=TTTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_35=579,20
PRIMER_RIGHT_35=1340,21
PRIMER_LEFT_35_TM=60.110
PRIMER_RIGHT_35_TM=62.704
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=10.9000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=762
PRIMER_PAIR_36_PENALTY=3.846987
PRIMER_LEFT_36_PENALTY=0.110392
PRIMER_RIGHT_36_PENALTY=3.736595
PRIMER_LEFT_36_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_36_SEQUENCE=CTTATGGAACCATTTGCTGA
PRIMER_LEFT_36=579,20
PRIMER_RIGHT_36=1457,20
PRIMER_LEFT_36_TM=60.110
PRIMER_RIGHT_36_TM=56.263
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=879
PRIMER_PAIR_37_PENALTY=3.873482
PRIMER_LEFT_37_PENALTY=0.110392
PRIMER_RIGHT_37_PENALTY=3.763090
PRIMER_LEFT_37_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_37_SEQUENCE=TCAGAGCGATCAGAAAGGAATG
PRIMER_LEFT_37=579,20
PRIMER_RIGHT_37=1544,22
PRIMER_LEFT_37_TM=60.110
PRIMER_RIGHT_37_TM=61.763
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=10.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=966
PRIMER_PAIR_38_PENALTY=3.913158
PRIMER_LEFT_38_PENALTY=2.390906
PRIMER_RIGHT_38_PENALTY=1.522251
PRIMER_LEFT_38_SEQUENCE=TCAAAAAGCTTAATCTCCCCAC
PRIMER_RIGHT_38_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_38=411,22
PRIMER_RIGHT_38=1339,20
PRIMER_LEFT_38_TM=59.609
PRIMER_RIGHT_38_TM=61.522
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=929
PRIMER_PAIR_39_PENALTY=3.913158
PRIMER_LEFT_39_PENALTY=2.390906
PRIMER_RIGHT_39_PENALTY=1.522251
PRIMER_LEFT_39_SEQUENCE=TCAAAAAGCTTAATCTCCCCAC
PRIMER_RIGHT_39_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_39=411,22
PRIMER_RIGHT_39=1340,20
PRIMER_LEFT_39_TM=59.609
PRIMER_RIGHT_39_TM=61.522
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=930
PRIMER_PAIR_40_PENALTY=4.036149
PRIMER_LEFT_40_PENALTY=1.766996
PRIMER_RIGHT_40_PENALTY=2.269153
PRIMER_LEFT_40_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_40_SEQUENCE=GAGCGATCAGAAAGGAATGTTT
PRIMER_LEFT_40=578,20
PRIMER_RIGHT_40=1541,22
PRIMER_LEFT_40_TM=61.767
PRIMER_RIGHT_40_TM=59.731
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=11.2000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=964
PRIMER_PAIR_41_PENALTY=4.144299
PRIMER_LEFT_41_PENALTY=0.440146
PRIMER_RIGHT_41_PENALTY=3.704154
PRIMER_LEFT_41_SEQUENCE=TGTTTTTGTGTGGTCGTTGG
PRIMER_RIGHT_41_SEQUENCE=TTTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_41=342,20
PRIMER_RIGHT_41=1340,21
PRIMER_LEFT_41_TM=60.440
PRIMER_RIGHT_41_TM=62.704
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=999
PRIMER_PAIR_42_PENALTY=4.265585
PRIMER_LEFT_42_PENALTY=3.228568
PRIMER_RIGHT_42_PENALTY=1.037017
PRIMER_LEFT_42_SEQUENCE=TGCTCTCTCGTTAGGTTTTGA
PRIMER_RIGHT_42_SEQUENCE=CAGAGCGATCAGAAAGGAATG
PRIMER_LEFT_42=565,21
PRIMER_RIGHT_42=1543,21
PRIMER_LEFT_42_TM=57.771
PRIMER_RIGHT_42_TM=59.963
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=979
PRIMER_PAIR_43_PENALTY=4.280978
PRIMER_LEFT_43_PENALTY=2.758727
PRIMER_RIGHT_43_PENALTY=1.522251
PRIMER_LEFT_43_SEQUENCE=TTGTTTTTGTGTGGTCGTTGG
PRIMER_RIGHT_43_SEQUENCE=TTAGCAATTGGGCCAGTCAA
PRIMER_LEFT_43=341,21
PRIMER_RIGHT_43=1339,20
PRIMER_LEFT_43_TM=61.759
PRIMER_RIGHT_43_TM=61.522
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=999
PRIMER_PAIR_44_PENALTY=4.280978
PRIMER_LEFT_44_PENALTY=2.758727
PRIMER_RIGHT_44_PENALTY=1.522251
PRIMER_LEFT_44_SEQUENCE=TTGTTTTTGTGTGGTCGTTGG
PRIMER_RIGHT_44_SEQUENCE=TTTAGCAATTGGGCCAGTCA
PRIMER_LEFT_44=341,21
PRIMER_RIGHT_44=1340,20
PRIMER_LEFT_44_TM=61.759
PRIMER_RIGHT_44_TM=61.522
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=1000
PRIMER_PAIR_45_PENALTY=4.300109
PRIMER_LEFT_45_PENALTY=0.110392
PRIMER_RIGHT_45_PENALTY=4.189717
PRIMER_LEFT_45_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_45_SEQUENCE=CGATCAGAAAGGAATGTTTG
PRIMER_LEFT_45=579,20
PRIMER_RIGHT_45=1538,20
PRIMER_LEFT_45_TM=60.110
PRIMER_RIGHT_45_TM=55.810
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=10.9000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=960
PRIMER_PAIR_46_PENALTY=4.317068
PRIMER_LEFT_46_PENALTY=3.228568
PRIMER_RIGHT_46_PENALTY=1.088501
PRIMER_LEFT_46_SEQUENCE=TGCTCTCTCGTTAGGTTTTGA
PRIMER_RIGHT_46_SEQUENCE=GCTTATGGAACCATTTGCTGA
PRIMER_LEFT_46=565,21
PRIMER_RIGHT_46=1458,21
PRIMER_LEFT_46_TM=57.771
PRIMER_RIGHT_46_TM=60.089
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=894
PRIMER_PAIR_47_PENALTY=4.353565
PRIMER_LEFT_47_PENALTY=0.110392
PRIMER_RIGHT_47_PENALTY=4.243173
PRIMER_LEFT_47_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_47_SEQUENCE=ATGGGCAGATGGGCTTATGG
PRIMER_LEFT_47=579,20
PRIMER_RIGHT_47=1470,20
PRIMER_LEFT_47_TM=60.110
PRIMER_RIGHT_47_TM=64.243
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=10.9000
PRIMER_RIGHT_47_END_STABILITY=7.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=892
PRIMER_PAIR_48_PENALTY=4.379166
PRIMER_LEFT_48_PENALTY=1.766996
PRIMER_RIGHT_48_PENALTY=2.612170
PRIMER_LEFT_48_SEQUENCE=GGTTTTGAACGTAGCTGGGG
PRIMER_RIGHT_48_SEQUENCE=AGCGATCAGAAAGGAATGTTTG
PRIMER_LEFT_48=578,20
PRIMER_RIGHT_48=1540,22
PRIMER_LEFT_48_TM=61.767
PRIMER_RIGHT_48_TM=60.612
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=11.2000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=963
PRIMER_PAIR_49_PENALTY=4.461001
PRIMER_LEFT_49_PENALTY=0.110392
PRIMER_RIGHT_49_PENALTY=4.350609
PRIMER_LEFT_49_SEQUENCE=GTTTTGAACGTAGCTGGGGA
PRIMER_RIGHT_49_SEQUENCE=GGGCAGATGGGCTTATGGAA
PRIMER_LEFT_49=579,20
PRIMER_RIGHT_49=1468,20
PRIMER_LEFT_49_TM=60.110
PRIMER_RIGHT_49_TM=64.351
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=10.9000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=890
=
SEQUENCE_ID=MAGIC.433_4_11384331_11384370_can-0_ler-0_3055_217630_TRUE
SEQUENCE_TARGET=950,139
SEQUENCE_TEMPLATE=GACAATTGCATTCAAAAGGGCGAGAGGGTCTGAAATTCTTAGCAATGTCTTNTTTCNTTTTTTTTTTTTNNTTTTTTTTTTTTTTTTTTTNATGNTNTAAAAAAGAAACTGAACATCATTATGTCTGAAAACACTGTTTCTTTTAATTGAAAAANTCATAATGTTGACNNNNNNNTTTCATCCTNNNNNNNNNNTATATGTAGCGGCTACATGTCTCCCGAATACGCAATGGAGGGGATATTCTCGGTAAAATCGGATGTTTTCNGCTTCGGGGTTTTGGTTCTTGAGATTGTCAGTGGAAAGAGGAACAGAGGATTCCACAACTCCGGCCAAGACAATAATCTTCTCGGCTACGNAAGCTTAAACCGCTTAAGNNNTTTATTTTGTCTCGTTCGATTGATAACACAATTTTTTTTATCTTTATTAACAGACGTGGGAGAATTGGAAAGAAGGAAAAGGGCTAGAGATCGTCGATTCGATAATTGTAGATTCCTCATCATCAATGTCATTGTTCCAACCACATGAAGTCTTAAGATGCATACAGATTGGTCTTTTATGTGTTCAAGANCGTGCNGAGGACAGACCAAAGATGNCGNCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGAACACATAANAGACCAATCTGTATGCATCTTAAGACTTCATGNGGTTNGAACANTGACANTGATGATGANNAATCTNCAATNANNGAATCGACGATNTCTAGCCCTTTTNCTTCTTTCCANTTNTCCCACGTCTGTNAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATNANAAACTTNAGNGGTTTAAGNTTACGTAGCCGAGAAGATTATTNTCNTGGCNNGAGTTGTNGAATCCTCTGTTCCTCTTTCCACTNACAATCTCAAGAANCANNACNCCNAAGCTGAAAACATCNGATTTTACCGAGAATATCCCNTCCATTGCGTATTCNGGAGACATGTAGCCGCTACATANNNNNNNNNNNAAGGATNNNNNNNAANAGTCAACATTATGANTTTTTNNNNNNNNNNNNNNNNNNNNNNCANACATAATGATGTTNAGTTNCTTACTAAGTTCCNACCACCTTCCTGGTNTTAGCTTCNGTCTCGTCCCTTTCAAATATCCTAGCCATNCCNAAATCCGAGATCTTTNGANTCATATTTTTATCAAGCAANACATTGCTTGCNTTCANATCTCTNNNNNNNNNNNNNNNNNTNGAATCTTGGTGAAGGTATAAAAGTCCTCGAGCAATACCATNGATAATGCTAAATCTCGTTTGCCANTTTAGCNNNTTNGAGCTTTGGGTTGTTTCTGTGTATAGATCACAACCAAATAAANTTAACTTATTNANATTAAAATTTAATTGTTGGTATACNACNACNAAAAAGGTNNGAGGCACTAACNGAAGAGATGAGANTCAAGGCTTCCANTNTCNAAGTANTCATANATCANTATCTTCTCANCCGCGTNGATGCAACANCNNAGAAGNCGGACAAGNTTNATNTGTTGAAGCNTNGCGATNAGCCTCACCTCNTTCNTGAANTCNNNNNNNNNNNNNNNNNNNNNCTNTGATAATCTTTTCACTGCNATCTNTTNCCCGTCANGTAANNTTCCCTGCTACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTACCTTGTATACAATACCGAAACCACCTCGTCCAAGNNNGTTGNAATCAGAGAAATTNTCNGTNGCCATGANAACAGNTTCAAACTCCGTGAGTGGAAGTTCCAANTCCTCNNCCTCNCCAANCAANNGTCTNNNACTTGANANNACCACNCCATTGGTTANANNNAATNCTTGAATTCTNTCTCGANANCNTGTTNNCATNGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.104140
PRIMER_LEFT_0_PENALTY=0.033680
PRIMER_RIGHT_0_PENALTY=2.070460
PRIMER_LEFT_0_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_0_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_0=425,20
PRIMER_RIGHT_0=1279,22
PRIMER_LEFT_0_TM=59.966
PRIMER_RIGHT_0_TM=59.930
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=855
PRIMER_PAIR_1_PENALTY=2.114582
PRIMER_LEFT_1_PENALTY=0.044122
PRIMER_RIGHT_1_PENALTY=2.070460
PRIMER_LEFT_1_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_1_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_1=431,20
PRIMER_RIGHT_1=1279,22
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=59.930
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=849
PRIMER_PAIR_2_PENALTY=2.186513
PRIMER_LEFT_2_PENALTY=0.033680
PRIMER_RIGHT_2_PENALTY=2.152833
PRIMER_LEFT_2_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_2_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_2=425,20
PRIMER_RIGHT_2=1404,21
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=58.847
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=980
PRIMER_PAIR_3_PENALTY=2.196955
PRIMER_LEFT_3_PENALTY=0.044122
PRIMER_RIGHT_3_PENALTY=2.152833
PRIMER_LEFT_3_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_3_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_3=431,20
PRIMER_RIGHT_3=1404,21
PRIMER_LEFT_3_TM=60.044
PRIMER_RIGHT_3_TM=58.847
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=974
PRIMER_PAIR_4_PENALTY=2.501517
PRIMER_LEFT_4_PENALTY=0.431057
PRIMER_RIGHT_4_PENALTY=2.070460
PRIMER_LEFT_4_SEQUENCE=AACTCCGGCCAAGACAATAA
PRIMER_RIGHT_4_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_4=321,20
PRIMER_RIGHT_4=1279,22
PRIMER_LEFT_4_TM=59.569
PRIMER_RIGHT_4_TM=59.930
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=959
PRIMER_PAIR_5_PENALTY=2.544154
PRIMER_LEFT_5_PENALTY=0.473695
PRIMER_RIGHT_5_PENALTY=2.070460
PRIMER_LEFT_5_SEQUENCE=ACGTGGGAGAATTGGAAAGA
PRIMER_RIGHT_5_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_5=430,20
PRIMER_RIGHT_5=1279,22
PRIMER_LEFT_5_TM=59.526
PRIMER_RIGHT_5_TM=59.930
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=850
PRIMER_PAIR_6_PENALTY=2.544154
PRIMER_LEFT_6_PENALTY=0.473695
PRIMER_RIGHT_6_PENALTY=2.070460
PRIMER_LEFT_6_SEQUENCE=GACGTGGGAGAATTGGAAAG
PRIMER_RIGHT_6_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_6=429,20
PRIMER_RIGHT_6=1279,22
PRIMER_LEFT_6_TM=59.526
PRIMER_RIGHT_6_TM=59.930
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=851
PRIMER_PAIR_7_PENALTY=2.544154
PRIMER_LEFT_7_PENALTY=0.473695
PRIMER_RIGHT_7_PENALTY=2.070460
PRIMER_LEFT_7_SEQUENCE=AGACGTGGGAGAATTGGAAA
PRIMER_RIGHT_7_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_7=428,20
PRIMER_RIGHT_7=1279,22
PRIMER_LEFT_7_TM=59.526
PRIMER_RIGHT_7_TM=59.930
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=852
PRIMER_PAIR_8_PENALTY=2.580791
PRIMER_LEFT_8_PENALTY=0.510331
PRIMER_RIGHT_8_PENALTY=2.070460
PRIMER_LEFT_8_SEQUENCE=ACAGACGTGGGAGAATTGGA
PRIMER_RIGHT_8_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_8=426,20
PRIMER_RIGHT_8=1279,22
PRIMER_LEFT_8_TM=60.510
PRIMER_RIGHT_8_TM=59.930
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=854
PRIMER_PAIR_9_PENALTY=2.626527
PRIMER_LEFT_9_PENALTY=0.473695
PRIMER_RIGHT_9_PENALTY=2.152833
PRIMER_LEFT_9_SEQUENCE=ACGTGGGAGAATTGGAAAGA
PRIMER_RIGHT_9_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_9=430,20
PRIMER_RIGHT_9=1404,21
PRIMER_LEFT_9_TM=59.526
PRIMER_RIGHT_9_TM=58.847
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=975
PRIMER_PAIR_10_PENALTY=2.626527
PRIMER_LEFT_10_PENALTY=0.473695
PRIMER_RIGHT_10_PENALTY=2.152833
PRIMER_LEFT_10_SEQUENCE=GACGTGGGAGAATTGGAAAG
PRIMER_RIGHT_10_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_10=429,20
PRIMER_RIGHT_10=1404,21
PRIMER_LEFT_10_TM=59.526
PRIMER_RIGHT_10_TM=58.847
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=976
PRIMER_PAIR_11_PENALTY=2.626527
PRIMER_LEFT_11_PENALTY=0.473695
PRIMER_RIGHT_11_PENALTY=2.152833
PRIMER_LEFT_11_SEQUENCE=AGACGTGGGAGAATTGGAAA
PRIMER_RIGHT_11_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_11=428,20
PRIMER_RIGHT_11=1404,21
PRIMER_LEFT_11_TM=59.526
PRIMER_RIGHT_11_TM=58.847
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=977
PRIMER_PAIR_12_PENALTY=2.663164
PRIMER_LEFT_12_PENALTY=0.510331
PRIMER_RIGHT_12_PENALTY=2.152833
PRIMER_LEFT_12_SEQUENCE=ACAGACGTGGGAGAATTGGA
PRIMER_RIGHT_12_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_12=426,20
PRIMER_RIGHT_12=1404,21
PRIMER_LEFT_12_TM=60.510
PRIMER_RIGHT_12_TM=58.847
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=979
PRIMER_PAIR_13_PENALTY=2.821071
PRIMER_LEFT_13_PENALTY=0.033680
PRIMER_RIGHT_13_PENALTY=2.787391
PRIMER_LEFT_13_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_13_SEQUENCE=TGGCTAGGATATTTGAAAGGG
PRIMER_LEFT_13=425,20
PRIMER_RIGHT_13=1279,21
PRIMER_LEFT_13_TM=59.966
PRIMER_RIGHT_13_TM=58.213
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.4000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=855
PRIMER_PAIR_14_PENALTY=2.831513
PRIMER_LEFT_14_PENALTY=0.044122
PRIMER_RIGHT_14_PENALTY=2.787391
PRIMER_LEFT_14_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_14_SEQUENCE=TGGCTAGGATATTTGAAAGGG
PRIMER_LEFT_14=431,20
PRIMER_RIGHT_14=1279,21
PRIMER_LEFT_14_TM=60.044
PRIMER_RIGHT_14_TM=58.213
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=849
PRIMER_PAIR_15_PENALTY=2.875023
PRIMER_LEFT_15_PENALTY=0.033680
PRIMER_RIGHT_15_PENALTY=2.841343
PRIMER_LEFT_15_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_15_SEQUENCE=ATGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_15=425,20
PRIMER_RIGHT_15=1405,22
PRIMER_LEFT_15_TM=59.966
PRIMER_RIGHT_15_TM=59.159
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=7.3000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=981
PRIMER_PAIR_16_PENALTY=2.885466
PRIMER_LEFT_16_PENALTY=0.044122
PRIMER_RIGHT_16_PENALTY=2.841343
PRIMER_LEFT_16_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_16_SEQUENCE=ATGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_16=431,20
PRIMER_RIGHT_16=1405,22
PRIMER_LEFT_16_TM=60.044
PRIMER_RIGHT_16_TM=59.159
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=975
PRIMER_PAIR_17_PENALTY=2.910911
PRIMER_LEFT_17_PENALTY=0.033680
PRIMER_RIGHT_17_PENALTY=2.877230
PRIMER_LEFT_17_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_17_SEQUENCE=GGATATTTGAAAGGGACGAGA
PRIMER_LEFT_17=425,20
PRIMER_RIGHT_17=1273,21
PRIMER_LEFT_17_TM=59.966
PRIMER_RIGHT_17_TM=58.123
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=849
PRIMER_PAIR_18_PENALTY=2.921353
PRIMER_LEFT_18_PENALTY=0.044122
PRIMER_RIGHT_18_PENALTY=2.877230
PRIMER_LEFT_18_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_18_SEQUENCE=GGATATTTGAAAGGGACGAGA
PRIMER_LEFT_18=431,20
PRIMER_RIGHT_18=1273,21
PRIMER_LEFT_18_TM=60.044
PRIMER_RIGHT_18_TM=58.123
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=843
PRIMER_PAIR_19_PENALTY=2.942575
PRIMER_LEFT_19_PENALTY=0.033680
PRIMER_RIGHT_19_PENALTY=2.908894
PRIMER_LEFT_19_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_19_SEQUENCE=AGGATATTTGAAAGGGACGAGA
PRIMER_LEFT_19=425,20
PRIMER_RIGHT_19=1274,22
PRIMER_LEFT_19_TM=59.966
PRIMER_RIGHT_19_TM=59.091
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=850
PRIMER_PAIR_20_PENALTY=2.953017
PRIMER_LEFT_20_PENALTY=0.044122
PRIMER_RIGHT_20_PENALTY=2.908894
PRIMER_LEFT_20_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_20_SEQUENCE=AGGATATTTGAAAGGGACGAGA
PRIMER_LEFT_20=431,20
PRIMER_RIGHT_20=1274,22
PRIMER_LEFT_20_TM=60.044
PRIMER_RIGHT_20_TM=59.091
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=844
PRIMER_PAIR_21_PENALTY=2.973002
PRIMER_LEFT_21_PENALTY=0.902542
PRIMER_RIGHT_21_PENALTY=2.070460
PRIMER_LEFT_21_SEQUENCE=TGGGAGAATTGGAAAGAAGG
PRIMER_RIGHT_21_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_21=433,20
PRIMER_RIGHT_21=1279,22
PRIMER_LEFT_21_TM=59.097
PRIMER_RIGHT_21_TM=59.930
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=847
PRIMER_PAIR_22_PENALTY=3.030148
PRIMER_LEFT_22_PENALTY=0.044122
PRIMER_RIGHT_22_PENALTY=2.986026
PRIMER_LEFT_22_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_22_SEQUENCE=GGCAAACGAGATTTAGCATT
PRIMER_LEFT_22=431,20
PRIMER_RIGHT_22=1429,20
PRIMER_LEFT_22_TM=60.044
PRIMER_RIGHT_22_TM=57.014
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=999
PRIMER_PAIR_23_PENALTY=3.055375
PRIMER_LEFT_23_PENALTY=0.902542
PRIMER_RIGHT_23_PENALTY=2.152833
PRIMER_LEFT_23_SEQUENCE=TGGGAGAATTGGAAAGAAGG
PRIMER_RIGHT_23_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_23=433,20
PRIMER_RIGHT_23=1404,21
PRIMER_LEFT_23_TM=59.097
PRIMER_RIGHT_23_TM=58.847
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=972
PRIMER_PAIR_24_PENALTY=3.073051
PRIMER_LEFT_24_PENALTY=0.033680
PRIMER_RIGHT_24_PENALTY=3.039370
PRIMER_LEFT_24_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_24_SEQUENCE=GGATATTTGAAAGGGACGAGAC
PRIMER_LEFT_24=425,20
PRIMER_RIGHT_24=1273,22
PRIMER_LEFT_24_TM=59.966
PRIMER_RIGHT_24_TM=58.961
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=849
PRIMER_PAIR_25_PENALTY=3.083493
PRIMER_LEFT_25_PENALTY=0.044122
PRIMER_RIGHT_25_PENALTY=3.039370
PRIMER_LEFT_25_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_25_SEQUENCE=GGATATTTGAAAGGGACGAGAC
PRIMER_LEFT_25=431,20
PRIMER_RIGHT_25=1273,22
PRIMER_LEFT_25_TM=60.044
PRIMER_RIGHT_25_TM=58.961
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=843
PRIMER_PAIR_26_PENALTY=3.091998
PRIMER_LEFT_26_PENALTY=1.021538
PRIMER_RIGHT_26_PENALTY=2.070460
PRIMER_LEFT_26_SEQUENCE=TAACAGACGTGGGAGAATTGG
PRIMER_RIGHT_26_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_26=424,21
PRIMER_RIGHT_26=1279,22
PRIMER_LEFT_26_TM=59.978
PRIMER_RIGHT_26_TM=59.930
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=9.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=856
PRIMER_PAIR_27_PENALTY=3.144360
PRIMER_LEFT_27_PENALTY=1.073900
PRIMER_RIGHT_27_PENALTY=2.070460
PRIMER_LEFT_27_SEQUENCE=GTGGGAGAATTGGAAAGAAGG
PRIMER_RIGHT_27_SEQUENCE=TGGCTAGGATATTTGAAAGGGA
PRIMER_LEFT_27=432,21
PRIMER_RIGHT_27=1279,22
PRIMER_LEFT_27_TM=59.926
PRIMER_RIGHT_27_TM=59.930
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=848
PRIMER_PAIR_28_PENALTY=3.174093
PRIMER_LEFT_28_PENALTY=0.033680
PRIMER_RIGHT_28_PENALTY=3.140413
PRIMER_LEFT_28_SEQUENCE=AACAGACGTGGGAGAATTGG
PRIMER_RIGHT_28_SEQUENCE=AGGATATTTGAAAGGGACGAGAC
PRIMER_LEFT_28=425,20
PRIMER_RIGHT_28=1274,23
PRIMER_LEFT_28_TM=59.966
PRIMER_RIGHT_28_TM=59.860
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=6.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=850
PRIMER_PAIR_29_PENALTY=3.174371
PRIMER_LEFT_29_PENALTY=1.021538
PRIMER_RIGHT_29_PENALTY=2.152833
PRIMER_LEFT_29_SEQUENCE=TAACAGACGTGGGAGAATTGG
PRIMER_RIGHT_29_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_29=424,21
PRIMER_RIGHT_29=1404,21
PRIMER_LEFT_29_TM=59.978
PRIMER_RIGHT_29_TM=58.847
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.4000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=981
PRIMER_PAIR_30_PENALTY=3.184535
PRIMER_LEFT_30_PENALTY=0.044122
PRIMER_RIGHT_30_PENALTY=3.140413
PRIMER_LEFT_30_SEQUENCE=CGTGGGAGAATTGGAAAGAA
PRIMER_RIGHT_30_SEQUENCE=AGGATATTTGAAAGGGACGAGAC
PRIMER_LEFT_30=431,20
PRIMER_RIGHT_30=1274,23
PRIMER_LEFT_30_TM=60.044
PRIMER_RIGHT_30_TM=59.860
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=43.478
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=844
PRIMER_PAIR_31_PENALTY=3.218448
PRIMER_LEFT_31_PENALTY=0.431057
PRIMER_RIGHT_31_PENALTY=2.787391
PRIMER_LEFT_31_SEQUENCE=AACTCCGGCCAAGACAATAA
PRIMER_RIGHT_31_SEQUENCE=TGGCTAGGATATTTGAAAGGG
PRIMER_LEFT_31=321,20
PRIMER_RIGHT_31=1279,21
PRIMER_LEFT_31_TM=59.569
PRIMER_RIGHT_31_TM=58.213
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.2000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=959
PRIMER_PAIR_32_PENALTY=3.226733
PRIMER_LEFT_32_PENALTY=1.073900
PRIMER_RIGHT_32_PENALTY=2.152833
PRIMER_LEFT_32_SEQUENCE=GTGGGAGAATTGGAAAGAAGG
PRIMER_RIGHT_32_SEQUENCE=TGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_32=432,21
PRIMER_RIGHT_32=1404,21
PRIMER_LEFT_32_TM=59.926
PRIMER_RIGHT_32_TM=58.847
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=973
PRIMER_PAIR_33_PENALTY=3.261085
PRIMER_LEFT_33_PENALTY=0.473695
PRIMER_RIGHT_33_PENALTY=2.787391
PRIMER_LEFT_33_SEQUENCE=ACGTGGGAGAATTGGAAAGA
PRIMER_RIGHT_33_SEQUENCE=TGGCTAGGATATTTGAAAGGG
PRIMER_LEFT_33=430,20
PRIMER_RIGHT_33=1279,21
PRIMER_LEFT_33_TM=59.526
PRIMER_RIGHT_33_TM=58.213
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=850
PRIMER_PAIR_34_PENALTY=3.261085
PRIMER_LEFT_34_PENALTY=0.473695
PRIMER_RIGHT_34_PENALTY=2.787391
PRIMER_LEFT_34_SEQUENCE=GACGTGGGAGAATTGGAAAG
PRIMER_RIGHT_34_SEQUENCE=TGGCTAGGATATTTGAAAGGG
PRIMER_LEFT_34=429,20
PRIMER_RIGHT_34=1279,21
PRIMER_LEFT_34_TM=59.526
PRIMER_RIGHT_34_TM=58.213
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=851
PRIMER_PAIR_35_PENALTY=3.261085
PRIMER_LEFT_35_PENALTY=0.473695
PRIMER_RIGHT_35_PENALTY=2.787391
PRIMER_LEFT_35_SEQUENCE=AGACGTGGGAGAATTGGAAA
PRIMER_RIGHT_35_SEQUENCE=TGGCTAGGATATTTGAAAGGG
PRIMER_LEFT_35=428,20
PRIMER_RIGHT_35=1279,21
PRIMER_LEFT_35_TM=59.526
PRIMER_RIGHT_35_TM=58.213
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=852
PRIMER_PAIR_36_PENALTY=3.297722
PRIMER_LEFT_36_PENALTY=0.510331
PRIMER_RIGHT_36_PENALTY=2.787391
PRIMER_LEFT_36_SEQUENCE=ACAGACGTGGGAGAATTGGA
PRIMER_RIGHT_36_SEQUENCE=TGGCTAGGATATTTGAAAGGG
PRIMER_LEFT_36=426,20
PRIMER_RIGHT_36=1279,21
PRIMER_LEFT_36_TM=60.510
PRIMER_RIGHT_36_TM=58.213
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=854
PRIMER_PAIR_37_PENALTY=3.308287
PRIMER_LEFT_37_PENALTY=0.431057
PRIMER_RIGHT_37_PENALTY=2.877230
PRIMER_LEFT_37_SEQUENCE=AACTCCGGCCAAGACAATAA
PRIMER_RIGHT_37_SEQUENCE=GGATATTTGAAAGGGACGAGA
PRIMER_LEFT_37=321,20
PRIMER_RIGHT_37=1273,21
PRIMER_LEFT_37_TM=59.569
PRIMER_RIGHT_37_TM=58.123
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.2000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=953
PRIMER_PAIR_38_PENALTY=3.315038
PRIMER_LEFT_38_PENALTY=0.473695
PRIMER_RIGHT_38_PENALTY=2.841343
PRIMER_LEFT_38_SEQUENCE=ACGTGGGAGAATTGGAAAGA
PRIMER_RIGHT_38_SEQUENCE=ATGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_38=430,20
PRIMER_RIGHT_38=1405,22
PRIMER_LEFT_38_TM=59.526
PRIMER_RIGHT_38_TM=59.159
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.3000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=976
PRIMER_PAIR_39_PENALTY=3.315038
PRIMER_LEFT_39_PENALTY=0.473695
PRIMER_RIGHT_39_PENALTY=2.841343
PRIMER_LEFT_39_SEQUENCE=GACGTGGGAGAATTGGAAAG
PRIMER_RIGHT_39_SEQUENCE=ATGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_39=429,20
PRIMER_RIGHT_39=1405,22
PRIMER_LEFT_39_TM=59.526
PRIMER_RIGHT_39_TM=59.159
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.3000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=977
PRIMER_PAIR_40_PENALTY=3.315038
PRIMER_LEFT_40_PENALTY=0.473695
PRIMER_RIGHT_40_PENALTY=2.841343
PRIMER_LEFT_40_SEQUENCE=AGACGTGGGAGAATTGGAAA
PRIMER_RIGHT_40_SEQUENCE=ATGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_40=428,20
PRIMER_RIGHT_40=1405,22
PRIMER_LEFT_40_TM=59.526
PRIMER_RIGHT_40_TM=59.159
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=7.3000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=978
PRIMER_PAIR_41_PENALTY=3.339951
PRIMER_LEFT_41_PENALTY=0.431057
PRIMER_RIGHT_41_PENALTY=2.908894
PRIMER_LEFT_41_SEQUENCE=AACTCCGGCCAAGACAATAA
PRIMER_RIGHT_41_SEQUENCE=AGGATATTTGAAAGGGACGAGA
PRIMER_LEFT_41=321,20
PRIMER_RIGHT_41=1274,22
PRIMER_LEFT_41_TM=59.569
PRIMER_RIGHT_41_TM=59.091
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.2000
PRIMER_RIGHT_41_END_STABILITY=8.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=954
PRIMER_PAIR_42_PENALTY=3.350925
PRIMER_LEFT_42_PENALTY=0.473695
PRIMER_RIGHT_42_PENALTY=2.877230
PRIMER_LEFT_42_SEQUENCE=ACGTGGGAGAATTGGAAAGA
PRIMER_RIGHT_42_SEQUENCE=GGATATTTGAAAGGGACGAGA
PRIMER_LEFT_42=430,20
PRIMER_RIGHT_42=1273,21
PRIMER_LEFT_42_TM=59.526
PRIMER_RIGHT_42_TM=58.123
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=844
PRIMER_PAIR_43_PENALTY=3.350925
PRIMER_LEFT_43_PENALTY=0.473695
PRIMER_RIGHT_43_PENALTY=2.877230
PRIMER_LEFT_43_SEQUENCE=GACGTGGGAGAATTGGAAAG
PRIMER_RIGHT_43_SEQUENCE=GGATATTTGAAAGGGACGAGA
PRIMER_LEFT_43=429,20
PRIMER_RIGHT_43=1273,21
PRIMER_LEFT_43_TM=59.526
PRIMER_RIGHT_43_TM=58.123
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=8.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=845
PRIMER_PAIR_44_PENALTY=3.350925
PRIMER_LEFT_44_PENALTY=0.473695
PRIMER_RIGHT_44_PENALTY=2.877230
PRIMER_LEFT_44_SEQUENCE=AGACGTGGGAGAATTGGAAA
PRIMER_RIGHT_44_SEQUENCE=GGATATTTGAAAGGGACGAGA
PRIMER_LEFT_44=428,20
PRIMER_RIGHT_44=1273,21
PRIMER_LEFT_44_TM=59.526
PRIMER_RIGHT_44_TM=58.123
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=846
PRIMER_PAIR_45_PENALTY=3.351674
PRIMER_LEFT_45_PENALTY=0.510331
PRIMER_RIGHT_45_PENALTY=2.841343
PRIMER_LEFT_45_SEQUENCE=ACAGACGTGGGAGAATTGGA
PRIMER_RIGHT_45_SEQUENCE=ATGGTATTGCTCGAGGACTTTT
PRIMER_LEFT_45=426,20
PRIMER_RIGHT_45=1405,22
PRIMER_LEFT_45_TM=60.510
PRIMER_RIGHT_45_TM=59.159
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=980
PRIMER_PAIR_46_PENALTY=3.382589
PRIMER_LEFT_46_PENALTY=0.473695
PRIMER_RIGHT_46_PENALTY=2.908894
PRIMER_LEFT_46_SEQUENCE=ACGTGGGAGAATTGGAAAGA
PRIMER_RIGHT_46_SEQUENCE=AGGATATTTGAAAGGGACGAGA
PRIMER_LEFT_46=430,20
PRIMER_RIGHT_46=1274,22
PRIMER_LEFT_46_TM=59.526
PRIMER_RIGHT_46_TM=59.091
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=845
PRIMER_PAIR_47_PENALTY=3.382589
PRIMER_LEFT_47_PENALTY=0.473695
PRIMER_RIGHT_47_PENALTY=2.908894
PRIMER_LEFT_47_SEQUENCE=GACGTGGGAGAATTGGAAAG
PRIMER_RIGHT_47_SEQUENCE=AGGATATTTGAAAGGGACGAGA
PRIMER_LEFT_47=429,20
PRIMER_RIGHT_47=1274,22
PRIMER_LEFT_47_TM=59.526
PRIMER_RIGHT_47_TM=59.091
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=846
PRIMER_PAIR_48_PENALTY=3.382589
PRIMER_LEFT_48_PENALTY=0.473695
PRIMER_RIGHT_48_PENALTY=2.908894
PRIMER_LEFT_48_SEQUENCE=AGACGTGGGAGAATTGGAAA
PRIMER_RIGHT_48_SEQUENCE=AGGATATTTGAAAGGGACGAGA
PRIMER_LEFT_48=428,20
PRIMER_RIGHT_48=1274,22
PRIMER_LEFT_48_TM=59.526
PRIMER_RIGHT_48_TM=59.091
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=847
PRIMER_PAIR_49_PENALTY=3.387561
PRIMER_LEFT_49_PENALTY=0.510331
PRIMER_RIGHT_49_PENALTY=2.877230
PRIMER_LEFT_49_SEQUENCE=ACAGACGTGGGAGAATTGGA
PRIMER_RIGHT_49_SEQUENCE=GGATATTTGAAAGGGACGAGA
PRIMER_LEFT_49=426,20
PRIMER_RIGHT_49=1273,21
PRIMER_LEFT_49_TM=60.510
PRIMER_RIGHT_49_TM=58.123
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=848
=
SEQUENCE_ID=MAGIC.446_5_18521276_18521583_po-0_zu-0_25223_18162_TRUE
SEQUENCE_TARGET=950,407
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTCGTTTNCATGTCGCTGATCCAGCGACCAAAANCAGNCGACTAATCGCTGAAACCAGTAANTGATCGNTAAAAATTTCAGCGACCAAAAATTNCGGTCGCTGNNNNNNNNNNNNNGTCGCTACTTTTGGTCGCTNNACCAGCNACAANCAAACNAACANCTTTTNGTCGCTGTNGCNGGTTTNAGTCGCNAGCGACANGNAAACGAACAGGNCNATNGTCTANANAAACCTANTTTGGGTTAATTAATAATTAATGTTCTANAATANANTANNNTATNNATAACCNTATNTATGTTGTACTGTGATAAATCNTACTGCATTTTCGCTATACAAGTTTTNATACGCTGGNNATTGTATAGTTGTATATACAACANTGATACGGNAANNANNATATTTTTAAAATGATTCGAAAACNNNNNNNATNTTNTTNTTTTNTCANAAANTNNTATTATTTTANTANTGTTTTCNATTTTTTAAATATTAGAATATTTTGATAACTGTTTTGTCAAGTTGGAAATTTTAAATATACAATGTCCTGCCGTAGATAATNTAATGTANNANCGTTATGTTATANNAACTGAATNTAAGAGTATTGNAAGTCCNACTNNGTCGAANAAATTGANNAAAAAGTGGNNAAAAATAATTGAGAGGCATCGACGTACATANTTGGCNACGAAAAAGTAGCTAAGCATTTGATTGGGAGATTCTGTTTCATCATATGCCTTCTGNTTTCTTGGGAAAATATAATNTCTNCTGCCCGTTTTTTGTTTGCTATGACAACTCTCAGTCTCNCATAACAGAATTCAGANTTTTTTTNTATATTTAGATAATCTGAAACTGATTTATATATTATCTTCTTCCATTTTGGGAGGTGATCATATAAGATAGAACAAGAANTAGAGNNNNNNNNNNNNNNNTTTTCAAGCAGAGAATANCTCNGNNTTTTTTACTGTAANTTNAGANCANATNNNNNNNNNTATTTNCANTNTGCACANAAAAAANNNNNNNATGTANNNNNNNNTTTCTATNTACCTTTTTATGTTAAAGTACCATTGAAGTATTTTTGTTTATGTCAACATGNTTGTGTNGATTTTACTNGNGAACAACNNATNNNNNNNNNNNNNNNNNNNCACTAGANNNNNNNNNCTNCAATGTTGTTGAGGTTNACGNGCTACNANAGTTGAATANNCATAAGAGATATTTTGAAAGTAAAANNTTCANTTTNTAAAGATAANATATGGGTNTNAAAAATATTTGTTTGCACGTTTATTCTTGGAGTATAAGAGATTTCATTCGACCATACTCATTTGTCCTTATCTTNNAATACTTTNTTTNGTTNTNGTCNTCATCCAAGAATCACCATATCTTATTATNNATTTTTTTNNGGTTCAGCTAGCAAGTAGCATCCCTTCGATAGACTGAGGAGAANAAANAAANAATTNTCTGGCTCTTACCNAATNTAAGATTTTNGCCATTTTCTCCCCNTTTTCTTTTGTGCAANCCATTACAATATCTATGGNTAGATCTTCANATTTCACATGTATGATATNTGAATGATTCCACTGCAANAAGTTATCAAACTAAGAGANNGCAATGNNTTTTNNNNTNNATTCATTTCACTGCAATGACGTTATTTTATTGTTGTTGGTGNNATANNCNGGCNGCGANTTTGAAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=26
PRIMER_RIGHT_NUM_RETURNED=26
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=26
PRIMER_PAIR_0_PENALTY=3.744997
PRIMER_LEFT_0_PENALTY=3.648142
PRIMER_RIGHT_0_PENALTY=0.096855
PRIMER_LEFT_0_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_0_SEQUENCE=CACCTCCCAAAATGGAAGAA
PRIMER_LEFT_0=592,23
PRIMER_RIGHT_0=1428,20
PRIMER_LEFT_0_TM=60.648
PRIMER_RIGHT_0_TM=59.903
PRIMER_LEFT_0_GC_PERCENT=43.478
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=5.7000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=837
PRIMER_PAIR_1_PENALTY=4.550684
PRIMER_LEFT_1_PENALTY=3.648142
PRIMER_RIGHT_1_PENALTY=0.902542
PRIMER_LEFT_1_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_1_SEQUENCE=CCTCCCAAAATGGAAGAAGA
PRIMER_LEFT_1=592,23
PRIMER_RIGHT_1=1426,20
PRIMER_LEFT_1_TM=60.648
PRIMER_RIGHT_1_TM=59.097
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=835
PRIMER_PAIR_2_PENALTY=4.722042
PRIMER_LEFT_2_PENALTY=3.648142
PRIMER_RIGHT_2_PENALTY=1.073900
PRIMER_LEFT_2_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_2_SEQUENCE=ACCTCCCAAAATGGAAGAAGA
PRIMER_LEFT_2=592,23
PRIMER_RIGHT_2=1427,21
PRIMER_LEFT_2_TM=60.648
PRIMER_RIGHT_2_TM=59.926
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=836
PRIMER_PAIR_3_PENALTY=4.897138
PRIMER_LEFT_3_PENALTY=3.648142
PRIMER_RIGHT_3_PENALTY=1.248996
PRIMER_LEFT_3_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_3_SEQUENCE=TGATCACCTCCCAAAATGGA
PRIMER_LEFT_3=592,23
PRIMER_RIGHT_3=1432,20
PRIMER_LEFT_3_TM=60.648
PRIMER_RIGHT_3_TM=61.249
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=841
PRIMER_PAIR_4_PENALTY=5.553732
PRIMER_LEFT_4_PENALTY=3.648142
PRIMER_RIGHT_4_PENALTY=1.905590
PRIMER_LEFT_4_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_4_SEQUENCE=ACCTCCCAAAATGGAAGAAG
PRIMER_LEFT_4=592,23
PRIMER_RIGHT_4=1427,20
PRIMER_LEFT_4_TM=60.648
PRIMER_RIGHT_4_TM=58.094
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=836
PRIMER_PAIR_5_PENALTY=5.817644
PRIMER_LEFT_5_PENALTY=5.720789
PRIMER_RIGHT_5_PENALTY=0.096855
PRIMER_LEFT_5_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_5_SEQUENCE=CACCTCCCAAAATGGAAGAA
PRIMER_LEFT_5=593,22
PRIMER_RIGHT_5=1428,20
PRIMER_LEFT_5_TM=56.279
PRIMER_RIGHT_5_TM=59.903
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=836
PRIMER_PAIR_6_PENALTY=6.105173
PRIMER_LEFT_6_PENALTY=3.648142
PRIMER_RIGHT_6_PENALTY=2.457031
PRIMER_LEFT_6_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_6_SEQUENCE=ATGATCACCTCCCAAAATGGA
PRIMER_LEFT_6=592,23
PRIMER_RIGHT_6=1433,21
PRIMER_LEFT_6_TM=60.648
PRIMER_RIGHT_6_TM=61.457
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=842
PRIMER_PAIR_7_PENALTY=6.360814
PRIMER_LEFT_7_PENALTY=3.648142
PRIMER_RIGHT_7_PENALTY=2.712672
PRIMER_LEFT_7_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_7_SEQUENCE=TCACCTCCCAAAATGGAAGAA
PRIMER_LEFT_7=592,23
PRIMER_RIGHT_7=1429,21
PRIMER_LEFT_7_TM=60.648
PRIMER_RIGHT_7_TM=61.713
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=838
PRIMER_PAIR_8_PENALTY=6.623331
PRIMER_LEFT_8_PENALTY=5.720789
PRIMER_RIGHT_8_PENALTY=0.902542
PRIMER_LEFT_8_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_8_SEQUENCE=CCTCCCAAAATGGAAGAAGA
PRIMER_LEFT_8=593,22
PRIMER_RIGHT_8=1426,20
PRIMER_LEFT_8_TM=56.279
PRIMER_RIGHT_8_TM=59.097
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=5.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=834
PRIMER_PAIR_9_PENALTY=6.794689
PRIMER_LEFT_9_PENALTY=5.720789
PRIMER_RIGHT_9_PENALTY=1.073900
PRIMER_LEFT_9_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_9_SEQUENCE=ACCTCCCAAAATGGAAGAAGA
PRIMER_LEFT_9=593,22
PRIMER_RIGHT_9=1427,21
PRIMER_LEFT_9_TM=56.279
PRIMER_RIGHT_9_TM=59.926
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=835
PRIMER_PAIR_10_PENALTY=6.969785
PRIMER_LEFT_10_PENALTY=5.720789
PRIMER_RIGHT_10_PENALTY=1.248996
PRIMER_LEFT_10_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_10_SEQUENCE=TGATCACCTCCCAAAATGGA
PRIMER_LEFT_10=593,22
PRIMER_RIGHT_10=1432,20
PRIMER_LEFT_10_TM=56.279
PRIMER_RIGHT_10_TM=61.249
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=5.7000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=840
PRIMER_PAIR_11_PENALTY=7.061801
PRIMER_LEFT_11_PENALTY=3.648142
PRIMER_RIGHT_11_PENALTY=3.413659
PRIMER_LEFT_11_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_11_SEQUENCE=TATGATCACCTCCCAAAATGGA
PRIMER_LEFT_11=592,23
PRIMER_RIGHT_11=1434,22
PRIMER_LEFT_11_TM=60.648
PRIMER_RIGHT_11_TM=61.414
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=843
PRIMER_PAIR_12_PENALTY=7.531327
PRIMER_LEFT_12_PENALTY=3.648142
PRIMER_RIGHT_12_PENALTY=3.883185
PRIMER_LEFT_12_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_12_SEQUENCE=ATCACCTCCCAAAATGGAAGAA
PRIMER_LEFT_12=592,23
PRIMER_RIGHT_12=1430,22
PRIMER_LEFT_12_TM=60.648
PRIMER_RIGHT_12_TM=61.883
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=839
PRIMER_PAIR_13_PENALTY=7.626378
PRIMER_LEFT_13_PENALTY=5.720789
PRIMER_RIGHT_13_PENALTY=1.905590
PRIMER_LEFT_13_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_13_SEQUENCE=ACCTCCCAAAATGGAAGAAG
PRIMER_LEFT_13=593,22
PRIMER_RIGHT_13=1427,20
PRIMER_LEFT_13_TM=56.279
PRIMER_RIGHT_13_TM=58.094
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=835
PRIMER_PAIR_14_PENALTY=7.785690
PRIMER_LEFT_14_PENALTY=3.648142
PRIMER_RIGHT_14_PENALTY=4.137548
PRIMER_LEFT_14_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_14_SEQUENCE=TATGATCACCTCCCAAAATG
PRIMER_LEFT_14=592,23
PRIMER_RIGHT_14=1434,20
PRIMER_LEFT_14_TM=60.648
PRIMER_RIGHT_14_TM=55.862
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=5.7000
PRIMER_RIGHT_14_END_STABILITY=7.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=843
PRIMER_PAIR_15_PENALTY=8.177820
PRIMER_LEFT_15_PENALTY=5.720789
PRIMER_RIGHT_15_PENALTY=2.457031
PRIMER_LEFT_15_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_15_SEQUENCE=ATGATCACCTCCCAAAATGGA
PRIMER_LEFT_15=593,22
PRIMER_RIGHT_15=1433,21
PRIMER_LEFT_15_TM=56.279
PRIMER_RIGHT_15_TM=61.457
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=5.7000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=841
PRIMER_PAIR_16_PENALTY=8.181915
PRIMER_LEFT_16_PENALTY=3.648142
PRIMER_RIGHT_16_PENALTY=4.533773
PRIMER_LEFT_16_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_16_SEQUENCE=CACCTCCCAAAATGGAAGAAGA
PRIMER_LEFT_16=592,23
PRIMER_RIGHT_16=1428,22
PRIMER_LEFT_16_TM=60.648
PRIMER_RIGHT_16_TM=62.534
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=837
PRIMER_PAIR_17_PENALTY=8.433461
PRIMER_LEFT_17_PENALTY=5.720789
PRIMER_RIGHT_17_PENALTY=2.712672
PRIMER_LEFT_17_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_17_SEQUENCE=TCACCTCCCAAAATGGAAGAA
PRIMER_LEFT_17=593,22
PRIMER_RIGHT_17=1429,21
PRIMER_LEFT_17_TM=56.279
PRIMER_RIGHT_17_TM=61.713
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=837
PRIMER_PAIR_18_PENALTY=9.134448
PRIMER_LEFT_18_PENALTY=5.720789
PRIMER_RIGHT_18_PENALTY=3.413659
PRIMER_LEFT_18_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_18_SEQUENCE=TATGATCACCTCCCAAAATGGA
PRIMER_LEFT_18=593,22
PRIMER_RIGHT_18=1434,22
PRIMER_LEFT_18_TM=56.279
PRIMER_RIGHT_18_TM=61.414
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=5.7000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=842
PRIMER_PAIR_19_PENALTY=9.603974
PRIMER_LEFT_19_PENALTY=5.720789
PRIMER_RIGHT_19_PENALTY=3.883185
PRIMER_LEFT_19_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_19_SEQUENCE=ATCACCTCCCAAAATGGAAGAA
PRIMER_LEFT_19=593,22
PRIMER_RIGHT_19=1430,22
PRIMER_LEFT_19_TM=56.279
PRIMER_RIGHT_19_TM=61.883
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=5.7000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=838
PRIMER_PAIR_20_PENALTY=9.858337
PRIMER_LEFT_20_PENALTY=5.720789
PRIMER_RIGHT_20_PENALTY=4.137548
PRIMER_LEFT_20_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_20_SEQUENCE=TATGATCACCTCCCAAAATG
PRIMER_LEFT_20=593,22
PRIMER_RIGHT_20=1434,20
PRIMER_LEFT_20_TM=56.279
PRIMER_RIGHT_20_TM=55.862
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=5.7000
PRIMER_RIGHT_20_END_STABILITY=7.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=842
PRIMER_PAIR_21_PENALTY=10.146705
PRIMER_LEFT_21_PENALTY=3.648142
PRIMER_RIGHT_21_PENALTY=6.498563
PRIMER_LEFT_21_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_21_SEQUENCE=GATCACCTCCCAAAATGGAAGAA
PRIMER_LEFT_21=592,23
PRIMER_RIGHT_21=1431,23
PRIMER_LEFT_21_TM=60.648
PRIMER_RIGHT_21_TM=63.499
PRIMER_LEFT_21_GC_PERCENT=43.478
PRIMER_RIGHT_21_GC_PERCENT=43.478
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=5.7000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=840
PRIMER_PAIR_22_PENALTY=10.254561
PRIMER_LEFT_22_PENALTY=5.720789
PRIMER_RIGHT_22_PENALTY=4.533773
PRIMER_LEFT_22_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_22_SEQUENCE=CACCTCCCAAAATGGAAGAAGA
PRIMER_LEFT_22=593,22
PRIMER_RIGHT_22=1428,22
PRIMER_LEFT_22_TM=56.279
PRIMER_RIGHT_22_TM=62.534
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=5.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=836
PRIMER_PAIR_23_PENALTY=12.207625
PRIMER_LEFT_23_PENALTY=3.648142
PRIMER_RIGHT_23_PENALTY=8.559483
PRIMER_LEFT_23_SEQUENCE=CGACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_23_SEQUENCE=CACCTCCCAAAATGGAAGAAGATAA
PRIMER_LEFT_23=592,23
PRIMER_RIGHT_23=1428,25
PRIMER_LEFT_23_TM=60.648
PRIMER_RIGHT_23_TM=63.559
PRIMER_LEFT_23_GC_PERCENT=43.478
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=5.7000
PRIMER_RIGHT_23_END_STABILITY=5.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=837
PRIMER_PAIR_24_PENALTY=12.219352
PRIMER_LEFT_24_PENALTY=5.720789
PRIMER_RIGHT_24_PENALTY=6.498563
PRIMER_LEFT_24_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_24_SEQUENCE=GATCACCTCCCAAAATGGAAGAA
PRIMER_LEFT_24=593,22
PRIMER_RIGHT_24=1431,23
PRIMER_LEFT_24_TM=56.279
PRIMER_RIGHT_24_TM=63.499
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=43.478
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=5.7000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=839
PRIMER_PAIR_25_PENALTY=14.280272
PRIMER_LEFT_25_PENALTY=5.720789
PRIMER_RIGHT_25_PENALTY=8.559483
PRIMER_LEFT_25_SEQUENCE=GACTAATCGCTGAAACCAGTAA
PRIMER_RIGHT_25_SEQUENCE=CACCTCCCAAAATGGAAGAAGATAA
PRIMER_LEFT_25=593,22
PRIMER_RIGHT_25=1428,25
PRIMER_LEFT_25_TM=56.279
PRIMER_RIGHT_25_TM=63.559
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=5.7000
PRIMER_RIGHT_25_END_STABILITY=5.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=836
=
SEQUENCE_ID=MAGIC.105_4_17560430_17560615_rsch-4_no-0_36672_107159_TRUE
SEQUENCE_TARGET=950,285
SEQUENCE_TEMPLATE=CAGACAGTNAAGAACATANNTATACNGATTTGTTAATGGGTATTTAATTTAAAATGCAGAGAAAGACANCANTTTGACGAGGCCCAACACATGCCNNCTACATGACCAAGTCACCAAACCANTTCTAAATTTCNNNAAACCAACATTGGANAGATGGAAACAGTGAAANTTTGNTTCTGTTGAACNTTNTANAGAAGCCGTGACATTGCTCTGCATCCAACTAATTGACCACANANTACTTTTANNTTCCANGAACACGTGGAATGTAAGAAACTNTATAGGTTTCTACACAATAGATCCNACATACGTCAATTATATCGTTTTACCAACAATGGCACGTGCCTTGCACATAGCTCGCAANGGAGTAGCTTTAGGCATAGTGATCCCTTCGCCTTCAGTCATNTCCACAAGTTCTNCACCAACTCTCTCCCACTCGAAAGACTGAATCAANCACCCAAGAGCAAGGCTCACTAACCGCTTCCCAAGCTCAGCTCCAGGACAAGCTCGTCGTCCCATCCCAAACGGCATTAGCTTTCGAGCCTCTCCTTCTTTCTCNNNNNNNNNNNNNNNGAACCTCTCTGGCTCTTCCCATAAACCTGGATCTCTATGCATCGCCCATACGTTTGTTAATAACAACGTGCGACGTGGCATATCGTAGCCCGCCACTTTACATTCATCCGATGAGAAATGAGGGAGNAGTAGTGGAACCGCAGGNTACAAACGTAATGTTTCTGACACAATGTTTTGGAGATAATGGAGATTTACAATATCTGATTCTTCAACTAACCGGTCTGAACCGNTTTTATCATCGATCTCCGCTCTCGCTTTCTCAAGTATCTCTGGATGGTTCANCAAATTTGACATTGCCCATTCCAACGTCACTGAAGATGTATCTGTCCCCGCAAGAACCANAGCCTGNNNNTATATATATCTCTNATAAGTAAANNCACTCCANATATANGGAGNATAATATTAGTTAATANGAACTTACNAGTATGATNCCTTTGATGATGANATCAGTATAGTACTCGGGTTCGGTTTCTTGGAAAGAAAGCAAATGATCGATCAAAGTTTGACCCTTTTCTTTCTCTGCACGTTTNTCATCAACCAGTTTTTGCAGNANTCTATCGAGTCGATTCCCCANGATTTTGGNCTGGTTCTCAAAGTTTGTGACCCAATTTATGGNCGGAAGATAATCAGCCAAATTTCCAGAACCGGCGCCGGCCATTATCTCCGCTATAAGTTCCCTCACGAGCTTGGCTTCATCATTNTCNTCTGTACCGNTACCNTANTATCNNTTNCCGGCTACCATCATGNTGATGTTGTTGAATGCCAAGTTGGTTANTAACGATTTCAACTCCACTTCAACAAATCCCTNNNGTGGGACGATTACTAGTCAAGTTTANNNNNNNNNNNNNNNNTTANTANATCTATATCAAANGTGTATNGTTCAGTTNCATCTANNNNNNNNNNNNNNNNTTATTTTATTTTCTGTNGTGACAAATTTGATGCATACAANTNANCTAACNGATAAATATANTNTTTTCCNGCCAATNANCGACAANTAANTAAATATNCGTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGAAAATCCTACTCACATGGACAAATTCANCCGCAATAGTCGATANATNAAANNNNNNNNNNNNNNNNNNNNNTTNTTTGGTAAATTNTAGTAATNCCAAAGNAAATNAAAGATAAATAATGAGTTAAATAAGAAGGTGGNTACGTACGTGNAAGGAGTCTCTAGAGAGATGTGTTATGAGCCGTCGGATCTCGTCTTTACGGATAGANNNAAAGGTACTNATTCTATGAGAAGAAAATATCTCGACGGCGGCGATGCGGCGGAGATTCCTCCAGTGGTCGCCGTAAGATGCTGCAATCATATTGGTAAAGTTATAGCCGACGTGTTTGGCCATGATGATATCAGGACGGTTCGCAAGAACGACGTCGTTTTTGGTGAAACATTCTTCGGCTATGGAGTGTGAGGAGATGACGTAAACGAGGCGGTTTCCCAGGNGGAGGTGGAAGATTGGAGCATTTCCAAGGGACTTNGAGATGGAGTGGAATGTTCGGTGGACCGGTTGCTTGAGGAGGTGGAGGTGGCCGATAACCGGTAAAGGGTAAGCCNGAGAAGGAGGAAGATTAGGTTTCCGGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.840752
PRIMER_LEFT_0_PENALTY=0.372343
PRIMER_RIGHT_0_PENALTY=1.468408
PRIMER_LEFT_0_SEQUENCE=AATTTGACATTGCCCATTCC
PRIMER_RIGHT_0_SEQUENCE=ATCCGACGGCTCATAACACA
PRIMER_LEFT_0=854,20
PRIMER_RIGHT_0=1801,20
PRIMER_LEFT_0_TM=59.628
PRIMER_RIGHT_0_TM=61.468
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=948
PRIMER_PAIR_1_PENALTY=1.908460
PRIMER_LEFT_1_PENALTY=0.066323
PRIMER_RIGHT_1_PENALTY=1.842136
PRIMER_LEFT_1_SEQUENCE=TGGCTCTTCCCATAAACCTG
PRIMER_RIGHT_1_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_1=579,20
PRIMER_RIGHT_1=1339,20
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=58.158
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=761
PRIMER_PAIR_2_PENALTY=1.937765
PRIMER_LEFT_2_PENALTY=0.095629
PRIMER_RIGHT_2_PENALTY=1.842136
PRIMER_LEFT_2_SEQUENCE=TAGCCCGCCACTTTACATTC
PRIMER_RIGHT_2_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_2=655,20
PRIMER_RIGHT_2=1339,20
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=58.158
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=685
PRIMER_PAIR_3_PENALTY=1.951070
PRIMER_LEFT_3_PENALTY=0.482662
PRIMER_RIGHT_3_PENALTY=1.468408
PRIMER_LEFT_3_SEQUENCE=GATGTATCTGTCCCCGCAAG
PRIMER_RIGHT_3_SEQUENCE=ATCCGACGGCTCATAACACA
PRIMER_LEFT_3=886,20
PRIMER_RIGHT_3=1801,20
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=61.468
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=916
PRIMER_PAIR_4_PENALTY=1.951070
PRIMER_LEFT_4_PENALTY=0.482662
PRIMER_RIGHT_4_PENALTY=1.468408
PRIMER_LEFT_4_SEQUENCE=AGATGTATCTGTCCCCGCAA
PRIMER_RIGHT_4_SEQUENCE=ATCCGACGGCTCATAACACA
PRIMER_LEFT_4=885,20
PRIMER_RIGHT_4=1801,20
PRIMER_LEFT_4_TM=60.483
PRIMER_RIGHT_4_TM=61.468
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=917
PRIMER_PAIR_5_PENALTY=1.951070
PRIMER_LEFT_5_PENALTY=0.482662
PRIMER_RIGHT_5_PENALTY=1.468408
PRIMER_LEFT_5_SEQUENCE=AAGATGTATCTGTCCCCGCA
PRIMER_RIGHT_5_SEQUENCE=ATCCGACGGCTCATAACACA
PRIMER_LEFT_5=884,20
PRIMER_RIGHT_5=1801,20
PRIMER_LEFT_5_TM=60.483
PRIMER_RIGHT_5_TM=61.468
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=11.7000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=918
PRIMER_PAIR_6_PENALTY=1.967071
PRIMER_LEFT_6_PENALTY=0.066323
PRIMER_RIGHT_6_PENALTY=1.900748
PRIMER_LEFT_6_SEQUENCE=TGGCTCTTCCCATAAACCTG
PRIMER_RIGHT_6_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_6=579,20
PRIMER_RIGHT_6=1336,20
PRIMER_LEFT_6_TM=60.066
PRIMER_RIGHT_6_TM=58.099
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=758
PRIMER_PAIR_7_PENALTY=1.991907
PRIMER_LEFT_7_PENALTY=0.149770
PRIMER_RIGHT_7_PENALTY=1.842136
PRIMER_LEFT_7_SEQUENCE=TCCGATGAGAAATGAGGGAG
PRIMER_RIGHT_7_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_7=676,20
PRIMER_RIGHT_7=1339,20
PRIMER_LEFT_7_TM=60.150
PRIMER_RIGHT_7_TM=58.158
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=664
PRIMER_PAIR_8_PENALTY=1.996376
PRIMER_LEFT_8_PENALTY=0.095629
PRIMER_RIGHT_8_PENALTY=1.900748
PRIMER_LEFT_8_SEQUENCE=TAGCCCGCCACTTTACATTC
PRIMER_RIGHT_8_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_8=655,20
PRIMER_RIGHT_8=1336,20
PRIMER_LEFT_8_TM=60.096
PRIMER_RIGHT_8_TM=58.099
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=682
PRIMER_PAIR_9_PENALTY=2.044808
PRIMER_LEFT_9_PENALTY=0.372343
PRIMER_RIGHT_9_PENALTY=1.672465
PRIMER_LEFT_9_SEQUENCE=AATTTGACATTGCCCATTCC
PRIMER_RIGHT_9_SEQUENCE=CGACGGCTCATAACACATCTC
PRIMER_LEFT_9=854,20
PRIMER_RIGHT_9=1798,21
PRIMER_LEFT_9_TM=59.628
PRIMER_RIGHT_9_TM=60.672
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=945
PRIMER_PAIR_10_PENALTY=2.050518
PRIMER_LEFT_10_PENALTY=0.149770
PRIMER_RIGHT_10_PENALTY=1.900748
PRIMER_LEFT_10_SEQUENCE=TCCGATGAGAAATGAGGGAG
PRIMER_RIGHT_10_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_10=676,20
PRIMER_RIGHT_10=1336,20
PRIMER_LEFT_10_TM=60.150
PRIMER_RIGHT_10_TM=58.099
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=661
PRIMER_PAIR_11_PENALTY=2.075360
PRIMER_LEFT_11_PENALTY=0.233224
PRIMER_RIGHT_11_PENALTY=1.842136
PRIMER_LEFT_11_SEQUENCE=GCTTTCGAGCCTCTCCTTCT
PRIMER_RIGHT_11_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_11=530,20
PRIMER_RIGHT_11=1339,20
PRIMER_LEFT_11_TM=60.233
PRIMER_RIGHT_11_TM=58.158
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=810
PRIMER_PAIR_12_PENALTY=2.075360
PRIMER_LEFT_12_PENALTY=0.233224
PRIMER_RIGHT_12_PENALTY=1.842136
PRIMER_LEFT_12_SEQUENCE=AGCTTTCGAGCCTCTCCTTC
PRIMER_RIGHT_12_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_12=529,20
PRIMER_RIGHT_12=1339,20
PRIMER_LEFT_12_TM=60.233
PRIMER_RIGHT_12_TM=58.158
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=811
PRIMER_PAIR_13_PENALTY=2.133972
PRIMER_LEFT_13_PENALTY=0.233224
PRIMER_RIGHT_13_PENALTY=1.900748
PRIMER_LEFT_13_SEQUENCE=GCTTTCGAGCCTCTCCTTCT
PRIMER_RIGHT_13_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_13=530,20
PRIMER_RIGHT_13=1336,20
PRIMER_LEFT_13_TM=60.233
PRIMER_RIGHT_13_TM=58.099
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=807
PRIMER_PAIR_14_PENALTY=2.133972
PRIMER_LEFT_14_PENALTY=0.233224
PRIMER_RIGHT_14_PENALTY=1.900748
PRIMER_LEFT_14_SEQUENCE=AGCTTTCGAGCCTCTCCTTC
PRIMER_RIGHT_14_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_14=529,20
PRIMER_RIGHT_14=1336,20
PRIMER_LEFT_14_TM=60.233
PRIMER_RIGHT_14_TM=58.099
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=808
PRIMER_PAIR_15_PENALTY=2.155127
PRIMER_LEFT_15_PENALTY=0.482662
PRIMER_RIGHT_15_PENALTY=1.672465
PRIMER_LEFT_15_SEQUENCE=GATGTATCTGTCCCCGCAAG
PRIMER_RIGHT_15_SEQUENCE=CGACGGCTCATAACACATCTC
PRIMER_LEFT_15=886,20
PRIMER_RIGHT_15=1798,21
PRIMER_LEFT_15_TM=60.483
PRIMER_RIGHT_15_TM=60.672
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.3000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=913
PRIMER_PAIR_16_PENALTY=2.155127
PRIMER_LEFT_16_PENALTY=0.482662
PRIMER_RIGHT_16_PENALTY=1.672465
PRIMER_LEFT_16_SEQUENCE=AGATGTATCTGTCCCCGCAA
PRIMER_RIGHT_16_SEQUENCE=CGACGGCTCATAACACATCTC
PRIMER_LEFT_16=885,20
PRIMER_RIGHT_16=1798,21
PRIMER_LEFT_16_TM=60.483
PRIMER_RIGHT_16_TM=60.672
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=10.5000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=914
PRIMER_PAIR_17_PENALTY=2.155127
PRIMER_LEFT_17_PENALTY=0.482662
PRIMER_RIGHT_17_PENALTY=1.672465
PRIMER_LEFT_17_SEQUENCE=AAGATGTATCTGTCCCCGCA
PRIMER_RIGHT_17_SEQUENCE=CGACGGCTCATAACACATCTC
PRIMER_LEFT_17=884,20
PRIMER_RIGHT_17=1798,21
PRIMER_LEFT_17_TM=60.483
PRIMER_RIGHT_17_TM=60.672
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=11.7000
PRIMER_RIGHT_17_END_STABILITY=6.3000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=915
PRIMER_PAIR_18_PENALTY=2.173001
PRIMER_LEFT_18_PENALTY=0.330865
PRIMER_RIGHT_18_PENALTY=1.842136
PRIMER_LEFT_18_SEQUENCE=CCGCCACTTTACATTCATCC
PRIMER_RIGHT_18_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_18=659,20
PRIMER_RIGHT_18=1339,20
PRIMER_LEFT_18_TM=60.331
PRIMER_RIGHT_18_TM=58.158
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=681
PRIMER_PAIR_19_PENALTY=2.173001
PRIMER_LEFT_19_PENALTY=0.330865
PRIMER_RIGHT_19_PENALTY=1.842136
PRIMER_LEFT_19_SEQUENCE=CCCGCCACTTTACATTCATC
PRIMER_RIGHT_19_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_19=658,20
PRIMER_RIGHT_19=1339,20
PRIMER_LEFT_19_TM=60.331
PRIMER_RIGHT_19_TM=58.158
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=682
PRIMER_PAIR_20_PENALTY=2.178179
PRIMER_LEFT_20_PENALTY=0.336042
PRIMER_RIGHT_20_PENALTY=1.842136
PRIMER_LEFT_20_SEQUENCE=GAACCTCTCTGGCTCTTCCC
PRIMER_RIGHT_20_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_20=570,20
PRIMER_RIGHT_20=1339,20
PRIMER_LEFT_20_TM=60.336
PRIMER_RIGHT_20_TM=58.158
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=770
PRIMER_PAIR_21_PENALTY=2.214480
PRIMER_LEFT_21_PENALTY=0.372343
PRIMER_RIGHT_21_PENALTY=1.842136
PRIMER_LEFT_21_SEQUENCE=AATTTGACATTGCCCATTCC
PRIMER_RIGHT_21_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_21=854,20
PRIMER_RIGHT_21=1339,20
PRIMER_LEFT_21_TM=59.628
PRIMER_RIGHT_21_TM=58.158
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=486
PRIMER_PAIR_22_PENALTY=2.231613
PRIMER_LEFT_22_PENALTY=0.330865
PRIMER_RIGHT_22_PENALTY=1.900748
PRIMER_LEFT_22_SEQUENCE=CCGCCACTTTACATTCATCC
PRIMER_RIGHT_22_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_22=659,20
PRIMER_RIGHT_22=1336,20
PRIMER_LEFT_22_TM=60.331
PRIMER_RIGHT_22_TM=58.099
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=678
PRIMER_PAIR_23_PENALTY=2.231613
PRIMER_LEFT_23_PENALTY=0.330865
PRIMER_RIGHT_23_PENALTY=1.900748
PRIMER_LEFT_23_SEQUENCE=CCCGCCACTTTACATTCATC
PRIMER_RIGHT_23_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_23=658,20
PRIMER_RIGHT_23=1336,20
PRIMER_LEFT_23_TM=60.331
PRIMER_RIGHT_23_TM=58.099
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=679
PRIMER_PAIR_24_PENALTY=2.236790
PRIMER_LEFT_24_PENALTY=0.336042
PRIMER_RIGHT_24_PENALTY=1.900748
PRIMER_LEFT_24_SEQUENCE=GAACCTCTCTGGCTCTTCCC
PRIMER_RIGHT_24_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_24=570,20
PRIMER_RIGHT_24=1336,20
PRIMER_LEFT_24_TM=60.336
PRIMER_RIGHT_24_TM=58.099
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=767
PRIMER_PAIR_25_PENALTY=2.273091
PRIMER_LEFT_25_PENALTY=0.372343
PRIMER_RIGHT_25_PENALTY=1.900748
PRIMER_LEFT_25_SEQUENCE=AATTTGACATTGCCCATTCC
PRIMER_RIGHT_25_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_25=854,20
PRIMER_RIGHT_25=1336,20
PRIMER_LEFT_25_TM=59.628
PRIMER_RIGHT_25_TM=58.099
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=483
PRIMER_PAIR_26_PENALTY=2.324798
PRIMER_LEFT_26_PENALTY=0.482662
PRIMER_RIGHT_26_PENALTY=1.842136
PRIMER_LEFT_26_SEQUENCE=GATGTATCTGTCCCCGCAAG
PRIMER_RIGHT_26_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_26=886,20
PRIMER_RIGHT_26=1339,20
PRIMER_LEFT_26_TM=60.483
PRIMER_RIGHT_26_TM=58.158
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=454
PRIMER_PAIR_27_PENALTY=2.324798
PRIMER_LEFT_27_PENALTY=0.482662
PRIMER_RIGHT_27_PENALTY=1.842136
PRIMER_LEFT_27_SEQUENCE=AGATGTATCTGTCCCCGCAA
PRIMER_RIGHT_27_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_27=885,20
PRIMER_RIGHT_27=1339,20
PRIMER_LEFT_27_TM=60.483
PRIMER_RIGHT_27_TM=58.158
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=10.5000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=455
PRIMER_PAIR_28_PENALTY=2.324798
PRIMER_LEFT_28_PENALTY=0.482662
PRIMER_RIGHT_28_PENALTY=1.842136
PRIMER_LEFT_28_SEQUENCE=AAGATGTATCTGTCCCCGCA
PRIMER_RIGHT_28_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_28=884,20
PRIMER_RIGHT_28=1339,20
PRIMER_LEFT_28_TM=60.483
PRIMER_RIGHT_28_TM=58.158
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=11.7000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=456
PRIMER_PAIR_29_PENALTY=2.383409
PRIMER_LEFT_29_PENALTY=0.482662
PRIMER_RIGHT_29_PENALTY=1.900748
PRIMER_LEFT_29_SEQUENCE=GATGTATCTGTCCCCGCAAG
PRIMER_RIGHT_29_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_29=886,20
PRIMER_RIGHT_29=1336,20
PRIMER_LEFT_29_TM=60.483
PRIMER_RIGHT_29_TM=58.099
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=451
PRIMER_PAIR_30_PENALTY=2.383409
PRIMER_LEFT_30_PENALTY=0.482662
PRIMER_RIGHT_30_PENALTY=1.900748
PRIMER_LEFT_30_SEQUENCE=AGATGTATCTGTCCCCGCAA
PRIMER_RIGHT_30_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_30=885,20
PRIMER_RIGHT_30=1336,20
PRIMER_LEFT_30_TM=60.483
PRIMER_RIGHT_30_TM=58.099
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=10.5000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=452
PRIMER_PAIR_31_PENALTY=2.383409
PRIMER_LEFT_31_PENALTY=0.482662
PRIMER_RIGHT_31_PENALTY=1.900748
PRIMER_LEFT_31_SEQUENCE=AAGATGTATCTGTCCCCGCA
PRIMER_RIGHT_31_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_31=884,20
PRIMER_RIGHT_31=1336,20
PRIMER_LEFT_31_TM=60.483
PRIMER_RIGHT_31_TM=58.099
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=11.7000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=453
PRIMER_PAIR_32_PENALTY=2.402585
PRIMER_LEFT_32_PENALTY=0.560449
PRIMER_RIGHT_32_PENALTY=1.842136
PRIMER_LEFT_32_SEQUENCE=ATCCGATGAGAAATGAGGGA
PRIMER_RIGHT_32_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_32=675,20
PRIMER_RIGHT_32=1339,20
PRIMER_LEFT_32_TM=59.440
PRIMER_RIGHT_32_TM=58.158
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=665
PRIMER_PAIR_33_PENALTY=2.461197
PRIMER_LEFT_33_PENALTY=0.560449
PRIMER_RIGHT_33_PENALTY=1.900748
PRIMER_LEFT_33_SEQUENCE=ATCCGATGAGAAATGAGGGA
PRIMER_RIGHT_33_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_33=675,20
PRIMER_RIGHT_33=1336,20
PRIMER_LEFT_33_TM=59.440
PRIMER_RIGHT_33_TM=58.099
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=662
PRIMER_PAIR_34_PENALTY=2.473620
PRIMER_LEFT_34_PENALTY=0.066323
PRIMER_RIGHT_34_PENALTY=2.407297
PRIMER_LEFT_34_SEQUENCE=TGGCTCTTCCCATAAACCTG
PRIMER_RIGHT_34_SEQUENCE=CAACTTGGCATTCAACAACATC
PRIMER_LEFT_34=579,20
PRIMER_RIGHT_34=1339,22
PRIMER_LEFT_34_TM=60.066
PRIMER_RIGHT_34_TM=60.407
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=6.3000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=761
PRIMER_PAIR_35_PENALTY=2.502926
PRIMER_LEFT_35_PENALTY=0.095629
PRIMER_RIGHT_35_PENALTY=2.407297
PRIMER_LEFT_35_SEQUENCE=TAGCCCGCCACTTTACATTC
PRIMER_RIGHT_35_SEQUENCE=CAACTTGGCATTCAACAACATC
PRIMER_LEFT_35=655,20
PRIMER_RIGHT_35=1339,22
PRIMER_LEFT_35_TM=60.096
PRIMER_RIGHT_35_TM=60.407
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=6.3000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=685
PRIMER_PAIR_36_PENALTY=2.557067
PRIMER_LEFT_36_PENALTY=0.149770
PRIMER_RIGHT_36_PENALTY=2.407297
PRIMER_LEFT_36_SEQUENCE=TCCGATGAGAAATGAGGGAG
PRIMER_RIGHT_36_SEQUENCE=CAACTTGGCATTCAACAACATC
PRIMER_LEFT_36=676,20
PRIMER_RIGHT_36=1339,22
PRIMER_LEFT_36_TM=60.150
PRIMER_RIGHT_36_TM=60.407
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.4000
PRIMER_RIGHT_36_END_STABILITY=6.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=664
PRIMER_PAIR_37_PENALTY=2.576301
PRIMER_LEFT_37_PENALTY=0.734164
PRIMER_RIGHT_37_PENALTY=1.842136
PRIMER_LEFT_37_SEQUENCE=TAGTGATCCCTTCGCCTTCA
PRIMER_RIGHT_37_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_37=377,20
PRIMER_RIGHT_37=1339,20
PRIMER_LEFT_37_TM=60.734
PRIMER_RIGHT_37_TM=58.158
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=963
PRIMER_PAIR_38_PENALTY=2.604830
PRIMER_LEFT_38_PENALTY=0.762694
PRIMER_RIGHT_38_PENALTY=1.842136
PRIMER_LEFT_38_SEQUENCE=AAGGCTCACTAACCGCTTCC
PRIMER_RIGHT_38_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_38=462,20
PRIMER_RIGHT_38=1339,20
PRIMER_LEFT_38_TM=60.763
PRIMER_RIGHT_38_TM=58.158
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=878
PRIMER_PAIR_39_PENALTY=2.613841
PRIMER_LEFT_39_PENALTY=1.145433
PRIMER_RIGHT_39_PENALTY=1.468408
PRIMER_LEFT_39_SEQUENCE=ATCTCCGCTCTCGCTTTCTC
PRIMER_RIGHT_39_SEQUENCE=ATCCGACGGCTCATAACACA
PRIMER_LEFT_39=811,20
PRIMER_RIGHT_39=1801,20
PRIMER_LEFT_39_TM=61.145
PRIMER_RIGHT_39_TM=61.468
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=991
PRIMER_PAIR_40_PENALTY=2.613841
PRIMER_LEFT_40_PENALTY=1.145433
PRIMER_RIGHT_40_PENALTY=1.468408
PRIMER_LEFT_40_SEQUENCE=GATCTCCGCTCTCGCTTTCT
PRIMER_RIGHT_40_SEQUENCE=ATCCGACGGCTCATAACACA
PRIMER_LEFT_40=810,20
PRIMER_RIGHT_40=1801,20
PRIMER_LEFT_40_TM=61.145
PRIMER_RIGHT_40_TM=61.468
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=992
PRIMER_PAIR_41_PENALTY=2.634912
PRIMER_LEFT_41_PENALTY=0.734164
PRIMER_RIGHT_41_PENALTY=1.900748
PRIMER_LEFT_41_SEQUENCE=TAGTGATCCCTTCGCCTTCA
PRIMER_RIGHT_41_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_41=377,20
PRIMER_RIGHT_41=1336,20
PRIMER_LEFT_41_TM=60.734
PRIMER_RIGHT_41_TM=58.099
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=960
PRIMER_PAIR_42_PENALTY=2.640521
PRIMER_LEFT_42_PENALTY=0.233224
PRIMER_RIGHT_42_PENALTY=2.407297
PRIMER_LEFT_42_SEQUENCE=GCTTTCGAGCCTCTCCTTCT
PRIMER_RIGHT_42_SEQUENCE=CAACTTGGCATTCAACAACATC
PRIMER_LEFT_42=530,20
PRIMER_RIGHT_42=1339,22
PRIMER_LEFT_42_TM=60.233
PRIMER_RIGHT_42_TM=60.407
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=810
PRIMER_PAIR_43_PENALTY=2.640521
PRIMER_LEFT_43_PENALTY=0.233224
PRIMER_RIGHT_43_PENALTY=2.407297
PRIMER_LEFT_43_SEQUENCE=AGCTTTCGAGCCTCTCCTTC
PRIMER_RIGHT_43_SEQUENCE=CAACTTGGCATTCAACAACATC
PRIMER_LEFT_43=529,20
PRIMER_RIGHT_43=1339,22
PRIMER_LEFT_43_TM=60.233
PRIMER_RIGHT_43_TM=60.407
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=811
PRIMER_PAIR_44_PENALTY=2.663442
PRIMER_LEFT_44_PENALTY=0.762694
PRIMER_RIGHT_44_PENALTY=1.900748
PRIMER_LEFT_44_SEQUENCE=AAGGCTCACTAACCGCTTCC
PRIMER_RIGHT_44_SEQUENCE=CTTGGCATTCAACAACATCA
PRIMER_LEFT_44=462,20
PRIMER_RIGHT_44=1336,20
PRIMER_LEFT_44_TM=60.763
PRIMER_RIGHT_44_TM=58.099
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=875
PRIMER_PAIR_45_PENALTY=2.666450
PRIMER_LEFT_45_PENALTY=0.095629
PRIMER_RIGHT_45_PENALTY=2.570821
PRIMER_LEFT_45_SEQUENCE=TAGCCCGCCACTTTACATTC
PRIMER_RIGHT_45_SEQUENCE=TCCATGTGAGTAGGATTTTCCA
PRIMER_LEFT_45=655,20
PRIMER_RIGHT_45=1632,22
PRIMER_LEFT_45_TM=60.096
PRIMER_RIGHT_45_TM=59.429
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=978
PRIMER_PAIR_46_PENALTY=2.692224
PRIMER_LEFT_46_PENALTY=0.850088
PRIMER_RIGHT_46_PENALTY=1.842136
PRIMER_LEFT_46_SEQUENCE=CATAGTGATCCCTTCGCCTT
PRIMER_RIGHT_46_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_46=375,20
PRIMER_RIGHT_46=1339,20
PRIMER_LEFT_46_TM=59.150
PRIMER_RIGHT_46_TM=58.158
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=965
PRIMER_PAIR_47_PENALTY=2.716636
PRIMER_LEFT_47_PENALTY=0.874500
PRIMER_RIGHT_47_PENALTY=1.842136
PRIMER_LEFT_47_SEQUENCE=GCTCTTCCCATAAACCTGGA
PRIMER_RIGHT_47_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_47=581,20
PRIMER_RIGHT_47=1339,20
PRIMER_LEFT_47_TM=59.126
PRIMER_RIGHT_47_TM=58.158
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=759
PRIMER_PAIR_48_PENALTY=2.716636
PRIMER_LEFT_48_PENALTY=0.874500
PRIMER_RIGHT_48_PENALTY=1.842136
PRIMER_LEFT_48_SEQUENCE=TCTGGCTCTTCCCATAAACC
PRIMER_RIGHT_48_SEQUENCE=CAACTTGGCATTCAACAACA
PRIMER_LEFT_48=577,20
PRIMER_RIGHT_48=1339,20
PRIMER_LEFT_48_TM=59.126
PRIMER_RIGHT_48_TM=58.158
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=763
PRIMER_PAIR_49_PENALTY=2.720592
PRIMER_LEFT_49_PENALTY=0.149770
PRIMER_RIGHT_49_PENALTY=2.570821
PRIMER_LEFT_49_SEQUENCE=TCCGATGAGAAATGAGGGAG
PRIMER_RIGHT_49_SEQUENCE=TCCATGTGAGTAGGATTTTCCA
PRIMER_LEFT_49=676,20
PRIMER_RIGHT_49=1632,22
PRIMER_LEFT_49_TM=60.150
PRIMER_RIGHT_49_TM=59.429
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=957
=
SEQUENCE_ID=MAGIC.426_3_12694644_12694775_wil-2_no-0_32566_20559_TRUE
SEQUENCE_TARGET=950,231
SEQUENCE_TEMPLATE=ATCNCTATTACTACATTATTATGTTGCNACTGCTACAAAAATTNTGTCGNCGCATCTATTAATTCNGNCGTACACACTTAAACTNTCNCAAATGGTAGCTACTTATTACTCAGTTATAAANTCGNNAAANGGTAACCAAGGGTTCTTGNCGNCGATTCACCCNAGAACTCGATNTNCATCTNATTCTTCTTCTNNNNNNNNNNNAAAANAATGTCAGCTAGTCAGAGCTNNAAATCTGATGCCAACAACAACAAGATGAGTTTCGNGCAGGGAAGTTATGGACATTNGAGCTNNNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4316712_4317214_ws-0_wil-2_64784_11541_TRUE
SEQUENCE_TARGET=950,602
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCCTAGTTGTGGGNTNGGAGTCTAATGAACTTTTTCTACAANTATTACATGAATTTAAAAGTAATATTTNTATAAAATTCANAAAGAGAAATGATTNNGTTTNCTACAGAAANAGATTTNGTACANCCTTTNGAANNTTTTCTTTAGTTNAGAATNGAAGTTTTGTGTAGATTTTGAAAACAAAAAGCNGTTNTTAGNGTTAAGAGNTTNTGGTTTAANAGCATCATTCTTGAGGGTTCCTNAGGTTGANTCTTAGGATAATTGAGTTAGAAATTTAATTNTTTTAATGANAATTTGNTAAGAGCGTCTCTAATATAAGAGACAATTAATNAGCTAAGAGCGGGATAGGTGTTNAAATTAGAGTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAACCCAATTTCTCACCGATTTCTCNCCGACTTNNTCGAAACCACAGAGTAAAACCNAATTNNCGAATCATCTNCAATCTGAAANTAAACTANTNNGCTNGAAAATTNTGNNAGNTGGGACTATNANGTTCCGTNGAGCTAGACCAATTCATCACAAGCGAGAAAGAAGGAGAANNANAGGAAATAGGAGAAGGGNTTTTTNGAAGCGATTNAGGCATTAGAGAGGATGANTAGAGAACCATCAATNTACGTTTAGTTTNAAAATCGTTAANGNTGTAGNAATCTCGANTGAAANNATTATTANTGTTTGACATAATATAAGAATTATACCCATAANTNTATATATACCTATAAAACTGCAAAGAANCANTTTTTCTAAATGATGGAAAANCNTGAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14340202_14340350_hi-0_kn-0_10612_26304_TRUE
SEQUENCE_TARGET=950,248
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_5909760_5910025_po-0_kn-0_137631_13830_TRUE
SEQUENCE_TARGET=950,365
SEQUENCE_TEMPLATE=NTTATTTTGAAATTTCNNTTTCNTNCGGTTNCNGTTCTTTCNGTTTTAAAATTTAGTATNNATTCGGTTATTTTGTTTTGAACAATTTNGNATATTTCAGTTATTGTTNAATATTTTCNNATANTTNTCNGNTANTTTCGANTANNNTTANTATTTTATATANNTTTTNAANTNTTTTTAATTATTNTGTNTAGAAATAACANATAGTTTTGAANATATTATTGTANTGCGGATATTTTANNCTACCCNAAATATTTNGGTTCGGNACCNATTNGNNTTNGNTTATTTNTAAAANTTTCGTNNNNNNNNGGTTCTATTATTTTTGGTGCGGTTCCAATTGGNTACATTCGNNNNNNTNNNTTTGCNNAATTGNNANNNATGAACTNAGAGGTCANNNNNNNTAAANTGCGAANNTNAANAATTGNCNNGTATTCATNCATTTCCCGCNTAAATCCTCCANCTTCTTCTTCGCCGGCNTNAATGGANTCNCCNACACANACGNCNGNNNACATAGAANCCNTGACGGAGTTTCTAGTAAAGCACTACNCCGATCAGCTCCGCGAAATCTCTCTCTCTTCNGANCCCAAACTTCACTACCCTCTCTTCNTCGAGTACGCAGAACTCGTTGACGATAATCCGTCTCTAGCNCGTCNAGTTTTCTCANATCCCGAACANTATCTTCGNCAATTCGACGANTCAGCCATTNTAGCTCACGTATGTACTCAATTTNCCCCNAAATTTGATTATACGAAATGGTGATTTCNANTAGCTTTGGGTGTAGAAAATTGCNNTGGAGCATATGAAGAAATTTNAGNAGAAGATTGGAATTGAGAAGAGATTTNTACATGTTCGTATAAACACNAGTGGATCTCCTCTAGAACGAAGCCCNNNTNTGTGTGNNNNNNTCTCTTGTTTTCGAGTTTGTTCTGCTTCTNTTAGNTGATTTTGATTTTGACACAGAGACNTTCCCGAGTATTGGCCGGGTTAGAGTAAAGCACCGNGGGATNCTGATGATGTTGAAAGGCACNGTGATTCGATCCGGGGCTGTNAAAATGTANGANGGAGAAAAGATGTATCGATGTCGAAAATGCAAGCACATGTNNNNNNNNNNNNNGTGGTTGAAACTGTATATTATNCATTTTNTCATCNNNNNNTNTTGTANTATNGCTGATTGAGTTNNTTTCTTCTATAGGTTTCCTATTTTCCCTGAGTTNGAATCAATCAANTCTATTGTGAAACCNCCNTTTTGTCCTTCTCAGGTTTGTNNNATTTCNTTCTTTGNCTAGCTAANCTNNTTTNNANTAGANNGTNTTCTGTTTTGCGANANCACTCNGACTANGGTCTCTNNNATTGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNACAGCGGTCAAAAGCNTGTGANGGCACAAACTTTGATCCTGTNGATGATACAGTAACACGNCATGATTATCAAGAAATCAAAATCCAAGAAAATACTCAGGTTTTAGGTGTTGGTGTTATCCCAAGGTCNATTCTAGTCGTCCTGAAAGATGACCTTGTTGACAATGTTAAAGCTGGAGGTAAACCAGTTTGTTTGTTTTTNTTCTGTGTCTTACATATNCANNATTGCGTNAATATTATGGCTCAGNGNAANCATTCNAAACGTATCAGCATAGCTGCTCCNCCTCCTGATATAACATATTNCNNTNNATGCAGATGATGTTGTTGTCTCTGGAATCTTGACTTCAAAATGGTCTCATGACTTNAAAGATGTTCGNTGNGACCTTGAACCTATGTTGATTGCCAATCATGTCAGGTAAATGGCTTGATAANTTTGATAGAGACCTACNANTCNATNTTATTAAGGANATCNTTANNANNNTCCTNAAANTTNTATNCANAANNGGTACTAGTTTGTTTGCCTGAAACAATTATTNAGGTCTTTGATGNGATTCTANTTTCTCNTNTTCTTAGTTACTATTNNTCATTANAGTTTNNTATTAACTNNCTTTCTNCCTTNTNTTCACCTGTTATCNTGCCACTNTCNAGAAGAACTAATGAGCTGAAATCAGAAATCGATATATCTGATGATTTGATCGAGAAGTTCAAGAACTTTTGGAGCCACTTCANAGATACCCCTTTGAAAGGTAAAAANNCANTGTTNNCTGNAANNTATTCCGCCTNTTTTNNTNGCNATTATTATNNACANNTATTCCCCTGCCNTNGANAANNNNTACGTATNNNTTTNGTNAANATTTATGCTAAATTCTTTANTGTTTTGGNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.910557
PRIMER_LEFT_0_PENALTY=0.736567
PRIMER_RIGHT_0_PENALTY=2.173990
PRIMER_LEFT_0_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_0_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_0=556,20
PRIMER_RIGHT_0=1507,21
PRIMER_LEFT_0_TM=59.263
PRIMER_RIGHT_0_TM=58.826
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=952
PRIMER_PAIR_1_PENALTY=2.910557
PRIMER_LEFT_1_PENALTY=0.736567
PRIMER_RIGHT_1_PENALTY=2.173990
PRIMER_LEFT_1_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_1=555,20
PRIMER_RIGHT_1=1507,21
PRIMER_LEFT_1_TM=59.263
PRIMER_RIGHT_1_TM=58.826
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=953
PRIMER_PAIR_2_PENALTY=2.968529
PRIMER_LEFT_2_PENALTY=0.736567
PRIMER_RIGHT_2_PENALTY=2.231961
PRIMER_LEFT_2_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_2_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_2=556,20
PRIMER_RIGHT_2=1506,20
PRIMER_LEFT_2_TM=59.263
PRIMER_RIGHT_2_TM=57.768
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=951
PRIMER_PAIR_3_PENALTY=2.968529
PRIMER_LEFT_3_PENALTY=0.736567
PRIMER_RIGHT_3_PENALTY=2.231961
PRIMER_LEFT_3_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_3_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_3=556,20
PRIMER_RIGHT_3=1507,20
PRIMER_LEFT_3_TM=59.263
PRIMER_RIGHT_3_TM=57.768
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=952
PRIMER_PAIR_4_PENALTY=2.968529
PRIMER_LEFT_4_PENALTY=0.736567
PRIMER_RIGHT_4_PENALTY=2.231961
PRIMER_LEFT_4_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_4_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_4=556,20
PRIMER_RIGHT_4=1508,20
PRIMER_LEFT_4_TM=59.263
PRIMER_RIGHT_4_TM=57.768
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=953
PRIMER_PAIR_5_PENALTY=2.968529
PRIMER_LEFT_5_PENALTY=0.736567
PRIMER_RIGHT_5_PENALTY=2.231961
PRIMER_LEFT_5_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_5_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_5=555,20
PRIMER_RIGHT_5=1506,20
PRIMER_LEFT_5_TM=59.263
PRIMER_RIGHT_5_TM=57.768
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=952
PRIMER_PAIR_6_PENALTY=2.968529
PRIMER_LEFT_6_PENALTY=0.736567
PRIMER_RIGHT_6_PENALTY=2.231961
PRIMER_LEFT_6_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_6_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_6=555,20
PRIMER_RIGHT_6=1507,20
PRIMER_LEFT_6_TM=59.263
PRIMER_RIGHT_6_TM=57.768
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=953
PRIMER_PAIR_7_PENALTY=2.968529
PRIMER_LEFT_7_PENALTY=0.736567
PRIMER_RIGHT_7_PENALTY=2.231961
PRIMER_LEFT_7_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_7_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_7=555,20
PRIMER_RIGHT_7=1508,20
PRIMER_LEFT_7_TM=59.263
PRIMER_RIGHT_7_TM=57.768
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=954
PRIMER_PAIR_8_PENALTY=3.053056
PRIMER_LEFT_8_PENALTY=0.736567
PRIMER_RIGHT_8_PENALTY=2.316489
PRIMER_LEFT_8_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_8_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_8=556,20
PRIMER_RIGHT_8=1509,21
PRIMER_LEFT_8_TM=59.263
PRIMER_RIGHT_8_TM=58.684
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=954
PRIMER_PAIR_9_PENALTY=3.053056
PRIMER_LEFT_9_PENALTY=0.736567
PRIMER_RIGHT_9_PENALTY=2.316489
PRIMER_LEFT_9_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_9_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_9=555,20
PRIMER_RIGHT_9=1509,21
PRIMER_LEFT_9_TM=59.263
PRIMER_RIGHT_9_TM=58.684
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=955
PRIMER_PAIR_10_PENALTY=3.128736
PRIMER_LEFT_10_PENALTY=0.736567
PRIMER_RIGHT_10_PENALTY=2.392169
PRIMER_LEFT_10_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_10_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_10=556,20
PRIMER_RIGHT_10=1508,21
PRIMER_LEFT_10_TM=59.263
PRIMER_RIGHT_10_TM=61.392
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=953
PRIMER_PAIR_11_PENALTY=3.128736
PRIMER_LEFT_11_PENALTY=0.736567
PRIMER_RIGHT_11_PENALTY=2.392169
PRIMER_LEFT_11_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_11_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_11=555,20
PRIMER_RIGHT_11=1508,21
PRIMER_LEFT_11_TM=59.263
PRIMER_RIGHT_11_TM=61.392
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=954
PRIMER_PAIR_12_PENALTY=3.253219
PRIMER_LEFT_12_PENALTY=0.736567
PRIMER_RIGHT_12_PENALTY=2.516652
PRIMER_LEFT_12_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_12_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_12=556,20
PRIMER_RIGHT_12=1510,22
PRIMER_LEFT_12_TM=59.263
PRIMER_RIGHT_12_TM=60.517
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=955
PRIMER_PAIR_13_PENALTY=3.253219
PRIMER_LEFT_13_PENALTY=0.736567
PRIMER_RIGHT_13_PENALTY=2.516652
PRIMER_LEFT_13_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_13_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_13=555,20
PRIMER_RIGHT_13=1510,22
PRIMER_LEFT_13_TM=59.263
PRIMER_RIGHT_13_TM=60.517
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=956
PRIMER_PAIR_14_PENALTY=3.319423
PRIMER_LEFT_14_PENALTY=1.145433
PRIMER_RIGHT_14_PENALTY=2.173990
PRIMER_LEFT_14_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_14_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_14=554,20
PRIMER_RIGHT_14=1507,21
PRIMER_LEFT_14_TM=61.145
PRIMER_RIGHT_14_TM=58.826
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=954
PRIMER_PAIR_15_PENALTY=3.377394
PRIMER_LEFT_15_PENALTY=1.145433
PRIMER_RIGHT_15_PENALTY=2.231961
PRIMER_LEFT_15_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_15_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_15=554,20
PRIMER_RIGHT_15=1506,20
PRIMER_LEFT_15_TM=61.145
PRIMER_RIGHT_15_TM=57.768
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=953
PRIMER_PAIR_16_PENALTY=3.377394
PRIMER_LEFT_16_PENALTY=1.145433
PRIMER_RIGHT_16_PENALTY=2.231961
PRIMER_LEFT_16_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_16_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_16=554,20
PRIMER_RIGHT_16=1507,20
PRIMER_LEFT_16_TM=61.145
PRIMER_RIGHT_16_TM=57.768
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=954
PRIMER_PAIR_17_PENALTY=3.377394
PRIMER_LEFT_17_PENALTY=1.145433
PRIMER_RIGHT_17_PENALTY=2.231961
PRIMER_LEFT_17_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_17_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_17=554,20
PRIMER_RIGHT_17=1508,20
PRIMER_LEFT_17_TM=61.145
PRIMER_RIGHT_17_TM=57.768
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=955
PRIMER_PAIR_18_PENALTY=3.407653
PRIMER_LEFT_18_PENALTY=1.233663
PRIMER_RIGHT_18_PENALTY=2.173990
PRIMER_LEFT_18_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_18_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_18=557,20
PRIMER_RIGHT_18=1507,21
PRIMER_LEFT_18_TM=58.766
PRIMER_RIGHT_18_TM=58.826
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=951
PRIMER_PAIR_19_PENALTY=3.413986
PRIMER_LEFT_19_PENALTY=1.239996
PRIMER_RIGHT_19_PENALTY=2.173990
PRIMER_LEFT_19_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_19_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_19=555,21
PRIMER_RIGHT_19=1507,21
PRIMER_LEFT_19_TM=60.240
PRIMER_RIGHT_19_TM=58.826
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=953
PRIMER_PAIR_20_PENALTY=3.461922
PRIMER_LEFT_20_PENALTY=1.145433
PRIMER_RIGHT_20_PENALTY=2.316489
PRIMER_LEFT_20_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_20_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_20=554,20
PRIMER_RIGHT_20=1509,21
PRIMER_LEFT_20_TM=61.145
PRIMER_RIGHT_20_TM=58.684
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=956
PRIMER_PAIR_21_PENALTY=3.465624
PRIMER_LEFT_21_PENALTY=1.233663
PRIMER_RIGHT_21_PENALTY=2.231961
PRIMER_LEFT_21_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_21_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_21=557,20
PRIMER_RIGHT_21=1506,20
PRIMER_LEFT_21_TM=58.766
PRIMER_RIGHT_21_TM=57.768
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=950
PRIMER_PAIR_22_PENALTY=3.465624
PRIMER_LEFT_22_PENALTY=1.233663
PRIMER_RIGHT_22_PENALTY=2.231961
PRIMER_LEFT_22_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_22_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_22=557,20
PRIMER_RIGHT_22=1507,20
PRIMER_LEFT_22_TM=58.766
PRIMER_RIGHT_22_TM=57.768
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=951
PRIMER_PAIR_23_PENALTY=3.465624
PRIMER_LEFT_23_PENALTY=1.233663
PRIMER_RIGHT_23_PENALTY=2.231961
PRIMER_LEFT_23_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_23_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_23=557,20
PRIMER_RIGHT_23=1508,20
PRIMER_LEFT_23_TM=58.766
PRIMER_RIGHT_23_TM=57.768
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=6.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=952
PRIMER_PAIR_24_PENALTY=3.471957
PRIMER_LEFT_24_PENALTY=1.239996
PRIMER_RIGHT_24_PENALTY=2.231961
PRIMER_LEFT_24_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_24_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_24=555,21
PRIMER_RIGHT_24=1506,20
PRIMER_LEFT_24_TM=60.240
PRIMER_RIGHT_24_TM=57.768
PRIMER_LEFT_24_GC_PERCENT=52.381
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=952
PRIMER_PAIR_25_PENALTY=3.471957
PRIMER_LEFT_25_PENALTY=1.239996
PRIMER_RIGHT_25_PENALTY=2.231961
PRIMER_LEFT_25_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_25_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_25=555,21
PRIMER_RIGHT_25=1507,20
PRIMER_LEFT_25_TM=60.240
PRIMER_RIGHT_25_TM=57.768
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=953
PRIMER_PAIR_26_PENALTY=3.471957
PRIMER_LEFT_26_PENALTY=1.239996
PRIMER_RIGHT_26_PENALTY=2.231961
PRIMER_LEFT_26_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_26_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_26=555,21
PRIMER_RIGHT_26=1508,20
PRIMER_LEFT_26_TM=60.240
PRIMER_RIGHT_26_TM=57.768
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=954
PRIMER_PAIR_27_PENALTY=3.492561
PRIMER_LEFT_27_PENALTY=0.736567
PRIMER_RIGHT_27_PENALTY=2.755994
PRIMER_LEFT_27_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_27_SEQUENCE=TGGGATAACACCAACACCTAAA
PRIMER_LEFT_27=556,20
PRIMER_RIGHT_27=1505,22
PRIMER_LEFT_27_TM=59.263
PRIMER_RIGHT_27_TM=59.244
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.3000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=950
PRIMER_PAIR_28_PENALTY=3.492561
PRIMER_LEFT_28_PENALTY=0.736567
PRIMER_RIGHT_28_PENALTY=2.755994
PRIMER_LEFT_28_SEQUENCE=TCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_28_SEQUENCE=TTGGGATAACACCAACACCTAA
PRIMER_LEFT_28=556,20
PRIMER_RIGHT_28=1506,22
PRIMER_LEFT_28_TM=59.263
PRIMER_RIGHT_28_TM=59.244
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=7.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=951
PRIMER_PAIR_29_PENALTY=3.492561
PRIMER_LEFT_29_PENALTY=0.736567
PRIMER_RIGHT_29_PENALTY=2.755994
PRIMER_LEFT_29_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_29_SEQUENCE=TGGGATAACACCAACACCTAAA
PRIMER_LEFT_29=555,20
PRIMER_RIGHT_29=1505,22
PRIMER_LEFT_29_TM=59.263
PRIMER_RIGHT_29_TM=59.244
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=6.3000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=951
PRIMER_PAIR_30_PENALTY=3.492561
PRIMER_LEFT_30_PENALTY=0.736567
PRIMER_RIGHT_30_PENALTY=2.755994
PRIMER_LEFT_30_SEQUENCE=CTCCGCGAAATCTCTCTCTC
PRIMER_RIGHT_30_SEQUENCE=TTGGGATAACACCAACACCTAA
PRIMER_LEFT_30=555,20
PRIMER_RIGHT_30=1506,22
PRIMER_LEFT_30_TM=59.263
PRIMER_RIGHT_30_TM=59.244
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.5000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=952
PRIMER_PAIR_31_PENALTY=3.537601
PRIMER_LEFT_31_PENALTY=1.145433
PRIMER_RIGHT_31_PENALTY=2.392169
PRIMER_LEFT_31_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_31_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_31=554,20
PRIMER_RIGHT_31=1508,21
PRIMER_LEFT_31_TM=61.145
PRIMER_RIGHT_31_TM=61.392
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=955
PRIMER_PAIR_32_PENALTY=3.550152
PRIMER_LEFT_32_PENALTY=1.233663
PRIMER_RIGHT_32_PENALTY=2.316489
PRIMER_LEFT_32_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_32_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_32=557,20
PRIMER_RIGHT_32=1509,21
PRIMER_LEFT_32_TM=58.766
PRIMER_RIGHT_32_TM=58.684
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=953
PRIMER_PAIR_33_PENALTY=3.554048
PRIMER_LEFT_33_PENALTY=1.380058
PRIMER_RIGHT_33_PENALTY=2.173990
PRIMER_LEFT_33_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_33_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_33=868,20
PRIMER_RIGHT_33=1507,21
PRIMER_LEFT_33_TM=58.620
PRIMER_RIGHT_33_TM=58.826
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=10.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=640
PRIMER_PAIR_34_PENALTY=3.556485
PRIMER_LEFT_34_PENALTY=1.239996
PRIMER_RIGHT_34_PENALTY=2.316489
PRIMER_LEFT_34_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_34_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_34=555,21
PRIMER_RIGHT_34=1509,21
PRIMER_LEFT_34_TM=60.240
PRIMER_RIGHT_34_TM=58.684
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=955
PRIMER_PAIR_35_PENALTY=3.612019
PRIMER_LEFT_35_PENALTY=1.380058
PRIMER_RIGHT_35_PENALTY=2.231961
PRIMER_LEFT_35_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_35_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_35=868,20
PRIMER_RIGHT_35=1506,20
PRIMER_LEFT_35_TM=58.620
PRIMER_RIGHT_35_TM=57.768
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=10.9000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=639
PRIMER_PAIR_36_PENALTY=3.612019
PRIMER_LEFT_36_PENALTY=1.380058
PRIMER_RIGHT_36_PENALTY=2.231961
PRIMER_LEFT_36_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_36_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_36=868,20
PRIMER_RIGHT_36=1507,20
PRIMER_LEFT_36_TM=58.620
PRIMER_RIGHT_36_TM=57.768
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=640
PRIMER_PAIR_37_PENALTY=3.612019
PRIMER_LEFT_37_PENALTY=1.380058
PRIMER_RIGHT_37_PENALTY=2.231961
PRIMER_LEFT_37_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_37_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_37=868,20
PRIMER_RIGHT_37=1508,20
PRIMER_LEFT_37_TM=58.620
PRIMER_RIGHT_37_TM=57.768
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=10.9000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=641
PRIMER_PAIR_38_PENALTY=3.625831
PRIMER_LEFT_38_PENALTY=1.233663
PRIMER_RIGHT_38_PENALTY=2.392169
PRIMER_LEFT_38_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_38_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_38=557,20
PRIMER_RIGHT_38=1508,21
PRIMER_LEFT_38_TM=58.766
PRIMER_RIGHT_38_TM=61.392
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=952
PRIMER_PAIR_39_PENALTY=3.632165
PRIMER_LEFT_39_PENALTY=1.239996
PRIMER_RIGHT_39_PENALTY=2.392169
PRIMER_LEFT_39_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_39_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_39=555,21
PRIMER_RIGHT_39=1508,21
PRIMER_LEFT_39_TM=60.240
PRIMER_RIGHT_39_TM=61.392
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=954
PRIMER_PAIR_40_PENALTY=3.662084
PRIMER_LEFT_40_PENALTY=1.145433
PRIMER_RIGHT_40_PENALTY=2.516652
PRIMER_LEFT_40_SEQUENCE=GCTCCGCGAAATCTCTCTCT
PRIMER_RIGHT_40_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_40=554,20
PRIMER_RIGHT_40=1510,22
PRIMER_LEFT_40_TM=61.145
PRIMER_RIGHT_40_TM=60.517
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=957
PRIMER_PAIR_41_PENALTY=3.696547
PRIMER_LEFT_41_PENALTY=1.380058
PRIMER_RIGHT_41_PENALTY=2.316489
PRIMER_LEFT_41_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_41_SEQUENCE=ACCTTGGGATAACACCAACAC
PRIMER_LEFT_41=868,20
PRIMER_RIGHT_41=1509,21
PRIMER_LEFT_41_TM=58.620
PRIMER_RIGHT_41_TM=58.684
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=10.9000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=642
PRIMER_PAIR_42_PENALTY=3.750314
PRIMER_LEFT_42_PENALTY=1.233663
PRIMER_RIGHT_42_PENALTY=2.516652
PRIMER_LEFT_42_SEQUENCE=CCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_42_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_42=557,20
PRIMER_RIGHT_42=1510,22
PRIMER_LEFT_42_TM=58.766
PRIMER_RIGHT_42_TM=60.517
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=954
PRIMER_PAIR_43_PENALTY=3.756648
PRIMER_LEFT_43_PENALTY=1.239996
PRIMER_RIGHT_43_PENALTY=2.516652
PRIMER_LEFT_43_SEQUENCE=CTCCGCGAAATCTCTCTCTCT
PRIMER_RIGHT_43_SEQUENCE=GACCTTGGGATAACACCAACAC
PRIMER_LEFT_43=555,21
PRIMER_RIGHT_43=1510,22
PRIMER_LEFT_43_TM=60.240
PRIMER_RIGHT_43_TM=60.517
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=956
PRIMER_PAIR_44_PENALTY=3.772226
PRIMER_LEFT_44_PENALTY=1.380058
PRIMER_RIGHT_44_PENALTY=2.392169
PRIMER_LEFT_44_SEQUENCE=TCTCCTCTAGAACGAAGCCC
PRIMER_RIGHT_44_SEQUENCE=CCTTGGGATAACACCAACACC
PRIMER_LEFT_44=868,20
PRIMER_RIGHT_44=1508,21
PRIMER_LEFT_44_TM=58.620
PRIMER_RIGHT_44_TM=61.392
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.9000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=641
PRIMER_PAIR_45_PENALTY=3.778313
PRIMER_LEFT_45_PENALTY=1.604323
PRIMER_RIGHT_45_PENALTY=2.173990
PRIMER_LEFT_45_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_45_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_45=557,21
PRIMER_RIGHT_45=1507,21
PRIMER_LEFT_45_TM=60.604
PRIMER_RIGHT_45_TM=58.826
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=951
PRIMER_PAIR_46_PENALTY=3.778313
PRIMER_LEFT_46_PENALTY=1.604323
PRIMER_RIGHT_46_PENALTY=2.173990
PRIMER_LEFT_46_SEQUENCE=TCCGCGAAATCTCTCTCTCTT
PRIMER_RIGHT_46_SEQUENCE=CTTGGGATAACACCAACACCT
PRIMER_LEFT_46=556,21
PRIMER_RIGHT_46=1507,21
PRIMER_LEFT_46_TM=60.604
PRIMER_RIGHT_46_TM=58.826
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=952
PRIMER_PAIR_47_PENALTY=3.836284
PRIMER_LEFT_47_PENALTY=1.604323
PRIMER_RIGHT_47_PENALTY=2.231961
PRIMER_LEFT_47_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_47_SEQUENCE=TTGGGATAACACCAACACCT
PRIMER_LEFT_47=557,21
PRIMER_RIGHT_47=1506,20
PRIMER_LEFT_47_TM=60.604
PRIMER_RIGHT_47_TM=57.768
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=950
PRIMER_PAIR_48_PENALTY=3.836284
PRIMER_LEFT_48_PENALTY=1.604323
PRIMER_RIGHT_48_PENALTY=2.231961
PRIMER_LEFT_48_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_48_SEQUENCE=CTTGGGATAACACCAACACC
PRIMER_LEFT_48=557,21
PRIMER_RIGHT_48=1507,20
PRIMER_LEFT_48_TM=60.604
PRIMER_RIGHT_48_TM=57.768
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=951
PRIMER_PAIR_49_PENALTY=3.836284
PRIMER_LEFT_49_PENALTY=1.604323
PRIMER_RIGHT_49_PENALTY=2.231961
PRIMER_LEFT_49_SEQUENCE=CCGCGAAATCTCTCTCTCTTC
PRIMER_RIGHT_49_SEQUENCE=CCTTGGGATAACACCAACAC
PRIMER_LEFT_49=557,21
PRIMER_RIGHT_49=1508,20
PRIMER_LEFT_49_TM=60.604
PRIMER_RIGHT_49_TM=57.768
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=952
=
SEQUENCE_ID=MAGIC.338_4_4598438_4598475_wu-0_ler-0_46770_43789_TRUE
SEQUENCE_TARGET=950,137
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTTGATTTCGTGGCAAAGTAAGTTGNTATATGANTGGNGAATTGTNTAATTATNNNNNNAAANNTNTATTNGTGTNTACATANTANATTATTGTNTANATTAACTACAATATTTNTAGTTTNCCTNCCNANGNTTTGNCNCTCACTATACAAGAAGCNNNTCCAAGAGAAGNGAATGAAATGATTAGANAGAANCAATANTNTGATCGTTTTCNANGGAAAACAATCTCNGACCTCTTTGAGCTTTCTNAGGTANTATTCTTNATAGTATAGACTAATTTANTTANATGNTTGAANCTTTTTAAATNATTGAANANNTNNNNNNNNNNNTANGTTNAGANGTGCAATATCATTTGTACCATANNNGAAAGTGGACANANACTATTCTTGNTTTTTCTTTTNTTGTCTGAAATGTAACAAAACAGNNNTTAAGATACCAAAANTNGAAAATGANATTNTTAAGAAAGGTAAGAAACAAATGTTTTGGTNTCCNNCATGCAAAGANGATACTCNTAAGGTTCNNCCAAGGNAAGTACCTAATAATTNGTCGTAANANATCATTTGTTAAATAANANTAGAGTCNATCAAANACNANTTTTTNTTCATTTCAANTATAGGTNNCTTTTAAANATTATNGTTATGNACAATACTNNAGACACNAAACTATTGGTGTTTGATAAAAATGCACAAGAGATAGTCGGTGTNTCTGCGGANGATNTNTTGGACGGTANNTNNGANGAGGTAACATCATTACAATTGCTANGTTATACNNNNNNNNNNNNNNNNNNNNNNNACANGAAGTATTGAANACTTTTGTATNNCTTTATAANAGATACATGATCNTANAAACTTGCCTCAAAGAATCCAAGNCTTAAAAGGGAAAANTTTCCNTNTGACNAAAAAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=15
PRIMER_RIGHT_NUM_RETURNED=15
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=15
PRIMER_PAIR_0_PENALTY=4.098892
PRIMER_LEFT_0_PENALTY=1.647773
PRIMER_RIGHT_0_PENALTY=2.451119
PRIMER_LEFT_0_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_0_SEQUENCE=CTTGGATTCTTTGAGGCAAG
PRIMER_LEFT_0=508,20
PRIMER_RIGHT_0=1376,20
PRIMER_LEFT_0_TM=58.352
PRIMER_RIGHT_0_TM=57.549
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=869
PRIMER_PAIR_1_PENALTY=4.199188
PRIMER_LEFT_1_PENALTY=1.647773
PRIMER_RIGHT_1_PENALTY=2.551415
PRIMER_LEFT_1_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_1_SEQUENCE=CTTGGATTCTTTGAGGCAAGT
PRIMER_LEFT_1=508,20
PRIMER_RIGHT_1=1376,21
PRIMER_LEFT_1_TM=58.352
PRIMER_RIGHT_1_TM=58.449
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=869
PRIMER_PAIR_2_PENALTY=4.254747
PRIMER_LEFT_2_PENALTY=1.647773
PRIMER_RIGHT_2_PENALTY=2.606973
PRIMER_LEFT_2_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_2_SEQUENCE=TTGGATTCTTTGAGGCAAGT
PRIMER_LEFT_2=508,20
PRIMER_RIGHT_2=1375,20
PRIMER_LEFT_2_TM=58.352
PRIMER_RIGHT_2_TM=57.393
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=868
PRIMER_PAIR_3_PENALTY=4.381702
PRIMER_LEFT_3_PENALTY=1.647773
PRIMER_RIGHT_3_PENALTY=2.733929
PRIMER_LEFT_3_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_3_SEQUENCE=CACCGACTATCTCTTGTGCATT
PRIMER_LEFT_3=508,20
PRIMER_RIGHT_3=1210,22
PRIMER_LEFT_3_TM=58.352
PRIMER_RIGHT_3_TM=59.266
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=703
PRIMER_PAIR_4_PENALTY=4.722468
PRIMER_LEFT_4_PENALTY=1.647773
PRIMER_RIGHT_4_PENALTY=3.074695
PRIMER_LEFT_4_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_4_SEQUENCE=ACACCGACTATCTCTTGTGCATT
PRIMER_LEFT_4=508,20
PRIMER_RIGHT_4=1211,23
PRIMER_LEFT_4_TM=58.352
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=704
PRIMER_PAIR_5_PENALTY=5.195120
PRIMER_LEFT_5_PENALTY=1.647773
PRIMER_RIGHT_5_PENALTY=3.547347
PRIMER_LEFT_5_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_5_SEQUENCE=CACCGACTATCTCTTGTGCATTT
PRIMER_LEFT_5=508,20
PRIMER_RIGHT_5=1210,23
PRIMER_LEFT_5_TM=58.352
PRIMER_RIGHT_5_TM=60.547
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=703
PRIMER_PAIR_6_PENALTY=5.245079
PRIMER_LEFT_6_PENALTY=1.647773
PRIMER_RIGHT_6_PENALTY=3.597306
PRIMER_LEFT_6_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_6_SEQUENCE=CCGACTATCTCTTGTGCATTTT
PRIMER_LEFT_6=508,20
PRIMER_RIGHT_6=1208,22
PRIMER_LEFT_6_TM=58.352
PRIMER_RIGHT_6_TM=58.403
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.909
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=701
PRIMER_PAIR_7_PENALTY=5.661096
PRIMER_LEFT_7_PENALTY=1.647773
PRIMER_RIGHT_7_PENALTY=4.013323
PRIMER_LEFT_7_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_7_SEQUENCE=CCGACTATCTCTTGTGCATTT
PRIMER_LEFT_7=508,20
PRIMER_RIGHT_7=1208,21
PRIMER_LEFT_7_TM=58.352
PRIMER_RIGHT_7_TM=56.987
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=701
PRIMER_PAIR_8_PENALTY=5.755482
PRIMER_LEFT_8_PENALTY=1.647773
PRIMER_RIGHT_8_PENALTY=4.107708
PRIMER_LEFT_8_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_8_SEQUENCE=ACCGACTATCTCTTGTGCATTT
PRIMER_LEFT_8=508,20
PRIMER_RIGHT_8=1209,22
PRIMER_LEFT_8_TM=58.352
PRIMER_RIGHT_8_TM=57.892
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=702
PRIMER_PAIR_9_PENALTY=6.221554
PRIMER_LEFT_9_PENALTY=1.647773
PRIMER_RIGHT_9_PENALTY=4.573781
PRIMER_LEFT_9_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_9_SEQUENCE=ACCGACTATCTCTTGTGCATT
PRIMER_LEFT_9=508,20
PRIMER_RIGHT_9=1209,21
PRIMER_LEFT_9_TM=58.352
PRIMER_RIGHT_9_TM=56.426
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=702
PRIMER_PAIR_10_PENALTY=6.240125
PRIMER_LEFT_10_PENALTY=1.647773
PRIMER_RIGHT_10_PENALTY=4.592352
PRIMER_LEFT_10_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_10_SEQUENCE=CCGACTATCTCTTGTGCATT
PRIMER_LEFT_10=508,20
PRIMER_RIGHT_10=1208,20
PRIMER_LEFT_10_TM=58.352
PRIMER_RIGHT_10_TM=55.408
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=701
PRIMER_PAIR_11_PENALTY=6.918633
PRIMER_LEFT_11_PENALTY=1.647773
PRIMER_RIGHT_11_PENALTY=5.270860
PRIMER_LEFT_11_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_11_SEQUENCE=ACACCGACTATCTCTTGTGCATTT
PRIMER_LEFT_11=508,20
PRIMER_RIGHT_11=1211,24
PRIMER_LEFT_11_TM=58.352
PRIMER_RIGHT_11_TM=61.271
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=41.667
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=704
PRIMER_PAIR_12_PENALTY=7.352191
PRIMER_LEFT_12_PENALTY=1.647773
PRIMER_RIGHT_12_PENALTY=5.704418
PRIMER_LEFT_12_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_12_SEQUENCE=CACCGACTATCTCTTGTGCATTTT
PRIMER_LEFT_12=508,20
PRIMER_RIGHT_12=1210,24
PRIMER_LEFT_12_TM=58.352
PRIMER_RIGHT_12_TM=61.704
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=41.667
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=703
PRIMER_PAIR_13_PENALTY=9.002758
PRIMER_LEFT_13_PENALTY=1.647773
PRIMER_RIGHT_13_PENALTY=7.354985
PRIMER_LEFT_13_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_13_SEQUENCE=ACACCGACTATCTCTTGTGCATTTT
PRIMER_LEFT_13=508,20
PRIMER_RIGHT_13=1211,25
PRIMER_LEFT_13_TM=58.352
PRIMER_RIGHT_13_TM=62.355
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=704
PRIMER_PAIR_14_PENALTY=9.402282
PRIMER_LEFT_14_PENALTY=1.647773
PRIMER_RIGHT_14_PENALTY=7.754508
PRIMER_LEFT_14_SEQUENCE=TCTTGATTTCGTGGCAAAGT
PRIMER_RIGHT_14_SEQUENCE=CACCGACTATCTCTTGTGCATTTTT
PRIMER_LEFT_14=508,20
PRIMER_RIGHT_14=1210,25
PRIMER_LEFT_14_TM=58.352
PRIMER_RIGHT_14_TM=62.755
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=703
=
SEQUENCE_ID=MAGIC.433_3_15385186_15385293_ws-0_oy-0_4123_752512_TRUE
SEQUENCE_TARGET=950,207
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4527578_4527704_po-0_edi-0_14288_24063_TRUE
SEQUENCE_TARGET=950,226
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCAGGACATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_6160418_6160559_ct-1_po-0_13086_69425_TRUE
SEQUENCE_TARGET=950,241
SEQUENCE_TEMPLATE=ATATCACAATTTCACTAATCGTCACNTGATATATGAATTCTTATTTGTAGTGACTAAATCTTATAGTACANATAGTATTATTACNTAGTAGTATCACACGTCACTGATGATATACTACGATGAATCAAAGGCCGGAGCAGACATAANAAAAATCATGATCCAGGGAAATAAATAACCAAAAGATTATTACCGTACGATTAGACTTTACAATTAATCTTCGTTACAATTCACGAGAAGATTCATGTTTCTTTATTTCCTAACAACAATATCTATTGTTTATAGTTCATAGGATCGTTCGTGTGGTAATCTGTTGACTGTACGCTTACCCATTAANATTTTCTNTTNTTGCTTTTTACTTTTTTCNTATAAAATTTATTAAAGTGATGTTTTTGCTTACTTCCATGCTCGCAAGAATCACAAACACACAATCATCATNAAAAAGAAATAAAGCAAATACAAAGAGAGGAATAGATAGGGAATGATAATNTATCAAAATAAGCAGTACAAACAAATAAAATTACCCACGATCTTTCTCCTAAAACTATTAATAAAGAAAAGGGTCTCACTGAATCGTACAAAGAGACATAAAAGAATGAATGAATCTTTGGGAAAAGAAAGGAAAGTGTCGGGGCTTCTTCTATGAATAAGCTATTCAGTTATTTTTCTTTTCTTTTGTCTTGTCTCTTCTCTACTCTAAAAGTCACTCTTCTTTCCTTTTCTCACCTTCCCACCCTCATATATATCTTGTCATTNTCCCCATTTGAANTTTATGGTGGCTAGGCTTTCTTCCTCATTCGTTAATCATACATCTTGTGATCTAATTCCTTTCATGCTTTCATCNAAAACCAAGCTTATNGAAGCTTTCAATTTAGTTTAAAATTTGTCAAAATGTTAAAATTCGTACGTAAGACATAGATGAATGAAANTTTAGCTAGTGTTTTATGGGAAATTTTTGTTTAAATAAATATTAATTGAATAATATGTTTTAAACTCTCATTNNNNAAAAATATAAAATTATCTCTTACTATAGGTAGAAGTAGAACCATGACCCGAATAGTCATTCAATTTTGAGGAATATACTGTATATATAATAGCAAATGTAAAGGCATATTGTTCGTATGCTAGTGAAGAGAAAAACAAANCAAGTGCCAAANCAATAATATTTTCCNACTCTCTTAGTCATTTCAACATCAACCCAATTCTTTTTGTCTCTCGCGCATTTATAACTCATAGAAACANATCCCCACATACATACACACACACNNNNNNNNATATATATAGATCATGCATGTGTGTGTAGATATTCTTTAGAAATAACGCATGAAATTAAAGGAAAGATGTAGAGTAATAACGAGTTCCCATTCTCGTAACAGATTATCCCATCACCAAAGCACCAAATAGCTTCATTCACCAACCAAATCAAGCAAGTNTCATGNTCTAAACCCTAAGTCACCACATCCACACAAATGAAAAAACTATTGGGAAAATCACANGTCTTTCTATTCTTTTCCTTTTNAAATTTGTTTGGTTCATTTTTAACAATTAAAGAAAGTGGTCCAAAGTGCATTTTAAGGCAAACCCTTATGAAATGTTAACATCAACACCATAAAGACACGCCTGCCCTTACTCCTANACAAAACCCTAATTATCCTTTTTCACTCTCCCTAACTCGGTTGAATAATTAAACTAATCATCTAATAACAAAAAGCTGTTTTTCTTGAAAGACTTCAATTAGGTTTAGTTCTTCAATGGCAGAAACTCATAGAAACAGTGAATGCCACTAATGTTCTTGTCTTTCTCACCATCGCTACAAAACCCTTCTTTTCTCAGTGGAAACAGCTCAAGTGTCTGTTCTTGATGATCCAATTCACCGTTGGAGCAATCTTCATGTTGTTCTTGATCTTTATTATCATCACCATTTCGATGACAAGTCTCTGTGTTCGTTGCTTCTCTCGTACGTAGAAATACNGGTGAAACCGTGACCGTATCTTTAGAAGCAGAGACAGCATTGTTGCTGTTGGACATGTTGGAGGAAACAGTAGNTGAANNNAGAATGAGGTTACGTTGATGATGATGATGTGAAGAAGAGTAGAAACTAGGTGGCAACTGCATCATCTGCCACGTGGTGTTTATCCCTAANA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.176659
PRIMER_LEFT_0_PENALTY=0.088413
PRIMER_RIGHT_0_PENALTY=0.088247
PRIMER_LEFT_0_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_0_SEQUENCE=TGCTTGATTTGGTTGGTGAA
PRIMER_LEFT_0=773,20
PRIMER_RIGHT_0=1425,20
PRIMER_LEFT_0_TM=60.088
PRIMER_RIGHT_0_TM=60.088
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=653
PRIMER_PAIR_1_PENALTY=0.176659
PRIMER_LEFT_1_PENALTY=0.088413
PRIMER_RIGHT_1_PENALTY=0.088247
PRIMER_LEFT_1_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCTTGATTTGGTTGGTGA
PRIMER_LEFT_1=773,20
PRIMER_RIGHT_1=1426,20
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=60.088
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=654
PRIMER_PAIR_2_PENALTY=0.239780
PRIMER_LEFT_2_PENALTY=0.151533
PRIMER_RIGHT_2_PENALTY=0.088247
PRIMER_LEFT_2_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_2_SEQUENCE=TGCTTGATTTGGTTGGTGAA
PRIMER_LEFT_2=771,20
PRIMER_RIGHT_2=1425,20
PRIMER_LEFT_2_TM=59.848
PRIMER_RIGHT_2_TM=60.088
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=655
PRIMER_PAIR_3_PENALTY=0.239780
PRIMER_LEFT_3_PENALTY=0.151533
PRIMER_RIGHT_3_PENALTY=0.088247
PRIMER_LEFT_3_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_3_SEQUENCE=TTGCTTGATTTGGTTGGTGA
PRIMER_LEFT_3=771,20
PRIMER_RIGHT_3=1426,20
PRIMER_LEFT_3_TM=59.848
PRIMER_RIGHT_3_TM=60.088
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=656
PRIMER_PAIR_4_PENALTY=0.334972
PRIMER_LEFT_4_PENALTY=0.246726
PRIMER_RIGHT_4_PENALTY=0.088247
PRIMER_LEFT_4_SEQUENCE=AAAGTGTCGGGGCTTCTTCT
PRIMER_RIGHT_4_SEQUENCE=TGCTTGATTTGGTTGGTGAA
PRIMER_LEFT_4=619,20
PRIMER_RIGHT_4=1425,20
PRIMER_LEFT_4_TM=60.247
PRIMER_RIGHT_4_TM=60.088
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=807
PRIMER_PAIR_5_PENALTY=0.334972
PRIMER_LEFT_5_PENALTY=0.246726
PRIMER_RIGHT_5_PENALTY=0.088247
PRIMER_LEFT_5_SEQUENCE=AAAGTGTCGGGGCTTCTTCT
PRIMER_RIGHT_5_SEQUENCE=TTGCTTGATTTGGTTGGTGA
PRIMER_LEFT_5=619,20
PRIMER_RIGHT_5=1426,20
PRIMER_LEFT_5_TM=60.247
PRIMER_RIGHT_5_TM=60.088
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=808
PRIMER_PAIR_6_PENALTY=0.394396
PRIMER_LEFT_6_PENALTY=0.306150
PRIMER_RIGHT_6_PENALTY=0.088247
PRIMER_LEFT_6_SEQUENCE=GTGTCGGGGCTTCTTCTATG
PRIMER_RIGHT_6_SEQUENCE=TGCTTGATTTGGTTGGTGAA
PRIMER_LEFT_6=622,20
PRIMER_RIGHT_6=1425,20
PRIMER_LEFT_6_TM=59.694
PRIMER_RIGHT_6_TM=60.088
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.9000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=804
PRIMER_PAIR_7_PENALTY=0.394396
PRIMER_LEFT_7_PENALTY=0.306150
PRIMER_RIGHT_7_PENALTY=0.088247
PRIMER_LEFT_7_SEQUENCE=GTGTCGGGGCTTCTTCTATG
PRIMER_RIGHT_7_SEQUENCE=TTGCTTGATTTGGTTGGTGA
PRIMER_LEFT_7=622,20
PRIMER_RIGHT_7=1426,20
PRIMER_LEFT_7_TM=59.694
PRIMER_RIGHT_7_TM=60.088
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=5.9000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=805
PRIMER_PAIR_8_PENALTY=0.419928
PRIMER_LEFT_8_PENALTY=0.004736
PRIMER_RIGHT_8_PENALTY=0.415192
PRIMER_LEFT_8_SEQUENCE=GCTCGCAAGAATCACAAACA
PRIMER_RIGHT_8_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_8=403,20
PRIMER_RIGHT_8=1222,20
PRIMER_LEFT_8_TM=59.995
PRIMER_RIGHT_8_TM=59.585
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=820
PRIMER_PAIR_9_PENALTY=0.419928
PRIMER_LEFT_9_PENALTY=0.004736
PRIMER_RIGHT_9_PENALTY=0.415192
PRIMER_LEFT_9_SEQUENCE=TGCTCGCAAGAATCACAAAC
PRIMER_RIGHT_9_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_9=402,20
PRIMER_RIGHT_9=1222,20
PRIMER_LEFT_9_TM=59.995
PRIMER_RIGHT_9_TM=59.585
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=821
PRIMER_PAIR_10_PENALTY=0.503605
PRIMER_LEFT_10_PENALTY=0.088413
PRIMER_RIGHT_10_PENALTY=0.415192
PRIMER_LEFT_10_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_10_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_10=773,20
PRIMER_RIGHT_10=1222,20
PRIMER_LEFT_10_TM=60.088
PRIMER_RIGHT_10_TM=59.585
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=450
PRIMER_PAIR_11_PENALTY=0.527298
PRIMER_LEFT_11_PENALTY=0.004736
PRIMER_RIGHT_11_PENALTY=0.522562
PRIMER_LEFT_11_SEQUENCE=GCTCGCAAGAATCACAAACA
PRIMER_RIGHT_11_SEQUENCE=AATGCGCGAGAGACAAAAAG
PRIMER_LEFT_11=403,20
PRIMER_RIGHT_11=1220,20
PRIMER_LEFT_11_TM=59.995
PRIMER_RIGHT_11_TM=60.523
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=818
PRIMER_PAIR_12_PENALTY=0.527298
PRIMER_LEFT_12_PENALTY=0.004736
PRIMER_RIGHT_12_PENALTY=0.522562
PRIMER_LEFT_12_SEQUENCE=TGCTCGCAAGAATCACAAAC
PRIMER_RIGHT_12_SEQUENCE=AATGCGCGAGAGACAAAAAG
PRIMER_LEFT_12=402,20
PRIMER_RIGHT_12=1220,20
PRIMER_LEFT_12_TM=59.995
PRIMER_RIGHT_12_TM=60.523
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=819
PRIMER_PAIR_13_PENALTY=0.555363
PRIMER_LEFT_13_PENALTY=0.467116
PRIMER_RIGHT_13_PENALTY=0.088247
PRIMER_LEFT_13_SEQUENCE=AAGAAAGGAAAGTGTCGGGG
PRIMER_RIGHT_13_SEQUENCE=TGCTTGATTTGGTTGGTGAA
PRIMER_LEFT_13=611,20
PRIMER_RIGHT_13=1425,20
PRIMER_LEFT_13_TM=60.467
PRIMER_RIGHT_13_TM=60.088
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=12.9000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=815
PRIMER_PAIR_14_PENALTY=0.555363
PRIMER_LEFT_14_PENALTY=0.467116
PRIMER_RIGHT_14_PENALTY=0.088247
PRIMER_LEFT_14_SEQUENCE=AAGAAAGGAAAGTGTCGGGG
PRIMER_RIGHT_14_SEQUENCE=TTGCTTGATTTGGTTGGTGA
PRIMER_LEFT_14=611,20
PRIMER_RIGHT_14=1426,20
PRIMER_LEFT_14_TM=60.467
PRIMER_RIGHT_14_TM=60.088
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=12.9000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=816
PRIMER_PAIR_15_PENALTY=0.566725
PRIMER_LEFT_15_PENALTY=0.151533
PRIMER_RIGHT_15_PENALTY=0.415192
PRIMER_LEFT_15_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_15_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_15=771,20
PRIMER_RIGHT_15=1222,20
PRIMER_LEFT_15_TM=59.848
PRIMER_RIGHT_15_TM=59.585
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=452
PRIMER_PAIR_16_PENALTY=0.661918
PRIMER_LEFT_16_PENALTY=0.246726
PRIMER_RIGHT_16_PENALTY=0.415192
PRIMER_LEFT_16_SEQUENCE=AAAGTGTCGGGGCTTCTTCT
PRIMER_RIGHT_16_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_16=619,20
PRIMER_RIGHT_16=1222,20
PRIMER_LEFT_16_TM=60.247
PRIMER_RIGHT_16_TM=59.585
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=604
PRIMER_PAIR_17_PENALTY=0.721341
PRIMER_LEFT_17_PENALTY=0.306150
PRIMER_RIGHT_17_PENALTY=0.415192
PRIMER_LEFT_17_SEQUENCE=GTGTCGGGGCTTCTTCTATG
PRIMER_RIGHT_17_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_17=622,20
PRIMER_RIGHT_17=1222,20
PRIMER_LEFT_17_TM=59.694
PRIMER_RIGHT_17_TM=59.585
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.9000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=601
PRIMER_PAIR_18_PENALTY=0.759730
PRIMER_LEFT_18_PENALTY=0.004736
PRIMER_RIGHT_18_PENALTY=0.754994
PRIMER_LEFT_18_SEQUENCE=TGCTCGCAAGAATCACAAAC
PRIMER_RIGHT_18_SEQUENCE=ATTTGGTGCTTTGGTGATGG
PRIMER_LEFT_18=402,20
PRIMER_RIGHT_18=1398,20
PRIMER_LEFT_18_TM=59.995
PRIMER_RIGHT_18_TM=60.755
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=997
PRIMER_PAIR_19_PENALTY=0.828712
PRIMER_LEFT_19_PENALTY=0.306150
PRIMER_RIGHT_19_PENALTY=0.522562
PRIMER_LEFT_19_SEQUENCE=GTGTCGGGGCTTCTTCTATG
PRIMER_RIGHT_19_SEQUENCE=AATGCGCGAGAGACAAAAAG
PRIMER_LEFT_19=622,20
PRIMER_RIGHT_19=1220,20
PRIMER_LEFT_19_TM=59.694
PRIMER_RIGHT_19_TM=60.523
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=599
PRIMER_PAIR_20_PENALTY=0.843407
PRIMER_LEFT_20_PENALTY=0.088413
PRIMER_RIGHT_20_PENALTY=0.754994
PRIMER_LEFT_20_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_20_SEQUENCE=ATTTGGTGCTTTGGTGATGG
PRIMER_LEFT_20=773,20
PRIMER_RIGHT_20=1398,20
PRIMER_LEFT_20_TM=60.088
PRIMER_RIGHT_20_TM=60.755
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=626
PRIMER_PAIR_21_PENALTY=0.882308
PRIMER_LEFT_21_PENALTY=0.467116
PRIMER_RIGHT_21_PENALTY=0.415192
PRIMER_LEFT_21_SEQUENCE=AAGAAAGGAAAGTGTCGGGG
PRIMER_RIGHT_21_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_21=611,20
PRIMER_RIGHT_21=1222,20
PRIMER_LEFT_21_TM=60.467
PRIMER_RIGHT_21_TM=59.585
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=12.9000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=612
PRIMER_PAIR_22_PENALTY=0.890585
PRIMER_LEFT_22_PENALTY=0.004736
PRIMER_RIGHT_22_PENALTY=0.885849
PRIMER_LEFT_22_SEQUENCE=GCTCGCAAGAATCACAAACA
PRIMER_RIGHT_22_SEQUENCE=AAATGCGCGAGAGACAAAAA
PRIMER_LEFT_22=403,20
PRIMER_RIGHT_22=1221,20
PRIMER_LEFT_22_TM=59.995
PRIMER_RIGHT_22_TM=60.886
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=819
PRIMER_PAIR_23_PENALTY=0.890585
PRIMER_LEFT_23_PENALTY=0.004736
PRIMER_RIGHT_23_PENALTY=0.885849
PRIMER_LEFT_23_SEQUENCE=TGCTCGCAAGAATCACAAAC
PRIMER_RIGHT_23_SEQUENCE=AAATGCGCGAGAGACAAAAA
PRIMER_LEFT_23=402,20
PRIMER_RIGHT_23=1221,20
PRIMER_LEFT_23_TM=59.995
PRIMER_RIGHT_23_TM=60.886
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=820
PRIMER_PAIR_24_PENALTY=0.906528
PRIMER_LEFT_24_PENALTY=0.151533
PRIMER_RIGHT_24_PENALTY=0.754994
PRIMER_LEFT_24_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_24_SEQUENCE=ATTTGGTGCTTTGGTGATGG
PRIMER_LEFT_24=771,20
PRIMER_RIGHT_24=1398,20
PRIMER_LEFT_24_TM=59.848
PRIMER_RIGHT_24_TM=60.755
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=628
PRIMER_PAIR_25_PENALTY=0.918604
PRIMER_LEFT_25_PENALTY=0.088413
PRIMER_RIGHT_25_PENALTY=0.830191
PRIMER_LEFT_25_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_25_SEQUENCE=CTTGCTTGATTTGGTTGGTG
PRIMER_LEFT_25=773,20
PRIMER_RIGHT_25=1427,20
PRIMER_LEFT_25_TM=60.088
PRIMER_RIGHT_25_TM=59.170
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=655
PRIMER_PAIR_26_PENALTY=0.940409
PRIMER_LEFT_26_PENALTY=0.852162
PRIMER_RIGHT_26_PENALTY=0.088247
PRIMER_LEFT_26_SEQUENCE=TTTTCTCACCTTCCCACCCT
PRIMER_RIGHT_26_SEQUENCE=TTGCTTGATTTGGTTGGTGA
PRIMER_LEFT_26=714,20
PRIMER_RIGHT_26=1426,20
PRIMER_LEFT_26_TM=60.852
PRIMER_RIGHT_26_TM=60.088
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=1.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.1000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=713
PRIMER_PAIR_27_PENALTY=0.940409
PRIMER_LEFT_27_PENALTY=0.852162
PRIMER_RIGHT_27_PENALTY=0.088247
PRIMER_LEFT_27_SEQUENCE=CCTTTTCTCACCTTCCCACC
PRIMER_RIGHT_27_SEQUENCE=TTGCTTGATTTGGTTGGTGA
PRIMER_LEFT_27=712,20
PRIMER_RIGHT_27=1426,20
PRIMER_LEFT_27_TM=60.852
PRIMER_RIGHT_27_TM=60.088
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=1.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=715
PRIMER_PAIR_28_PENALTY=0.974262
PRIMER_LEFT_28_PENALTY=0.088413
PRIMER_RIGHT_28_PENALTY=0.885849
PRIMER_LEFT_28_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_28_SEQUENCE=AAATGCGCGAGAGACAAAAA
PRIMER_LEFT_28=773,20
PRIMER_RIGHT_28=1221,20
PRIMER_LEFT_28_TM=60.088
PRIMER_RIGHT_28_TM=60.886
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=449
PRIMER_PAIR_29_PENALTY=0.976398
PRIMER_LEFT_29_PENALTY=0.888152
PRIMER_RIGHT_29_PENALTY=0.088247
PRIMER_LEFT_29_SEQUENCE=TCCTTTTCTCACCTTCCCAC
PRIMER_RIGHT_29_SEQUENCE=TGCTTGATTTGGTTGGTGAA
PRIMER_LEFT_29=711,20
PRIMER_RIGHT_29=1425,20
PRIMER_LEFT_29_TM=59.112
PRIMER_RIGHT_29_TM=60.088
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=1.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=715
PRIMER_PAIR_30_PENALTY=0.981725
PRIMER_LEFT_30_PENALTY=0.151533
PRIMER_RIGHT_30_PENALTY=0.830191
PRIMER_LEFT_30_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_30_SEQUENCE=CTTGCTTGATTTGGTTGGTG
PRIMER_LEFT_30=771,20
PRIMER_RIGHT_30=1427,20
PRIMER_LEFT_30_TM=59.848
PRIMER_RIGHT_30_TM=59.170
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=657
PRIMER_PAIR_31_PENALTY=0.989678
PRIMER_LEFT_31_PENALTY=0.467116
PRIMER_RIGHT_31_PENALTY=0.522562
PRIMER_LEFT_31_SEQUENCE=AAGAAAGGAAAGTGTCGGGG
PRIMER_RIGHT_31_SEQUENCE=AATGCGCGAGAGACAAAAAG
PRIMER_LEFT_31=611,20
PRIMER_RIGHT_31=1220,20
PRIMER_LEFT_31_TM=60.467
PRIMER_RIGHT_31_TM=60.523
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=12.9000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=610
PRIMER_PAIR_32_PENALTY=0.990432
PRIMER_LEFT_32_PENALTY=0.575240
PRIMER_RIGHT_32_PENALTY=0.415192
PRIMER_LEFT_32_SEQUENCE=ATGCTCGCAAGAATCACAAA
PRIMER_RIGHT_32_SEQUENCE=TAAATGCGCGAGAGACAAAA
PRIMER_LEFT_32=401,20
PRIMER_RIGHT_32=1222,20
PRIMER_LEFT_32_TM=59.425
PRIMER_RIGHT_32_TM=59.585
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=822
PRIMER_PAIR_33_PENALTY=1.001720
PRIMER_LEFT_33_PENALTY=0.246726
PRIMER_RIGHT_33_PENALTY=0.754994
PRIMER_LEFT_33_SEQUENCE=AAAGTGTCGGGGCTTCTTCT
PRIMER_RIGHT_33_SEQUENCE=ATTTGGTGCTTTGGTGATGG
PRIMER_LEFT_33=619,20
PRIMER_RIGHT_33=1398,20
PRIMER_LEFT_33_TM=60.247
PRIMER_RIGHT_33_TM=60.755
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=8.1000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=780
PRIMER_PAIR_34_PENALTY=1.037382
PRIMER_LEFT_34_PENALTY=0.151533
PRIMER_RIGHT_34_PENALTY=0.885849
PRIMER_LEFT_34_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_34_SEQUENCE=AAATGCGCGAGAGACAAAAA
PRIMER_LEFT_34=771,20
PRIMER_RIGHT_34=1221,20
PRIMER_LEFT_34_TM=59.848
PRIMER_RIGHT_34_TM=60.886
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=451
PRIMER_PAIR_35_PENALTY=1.038350
PRIMER_LEFT_35_PENALTY=0.088413
PRIMER_RIGHT_35_PENALTY=0.949937
PRIMER_LEFT_35_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_35_SEQUENCE=GGCGTGTCTTTATGGTGTTG
PRIMER_LEFT_35=773,20
PRIMER_RIGHT_35=1617,20
PRIMER_LEFT_35_TM=60.088
PRIMER_RIGHT_35_TM=59.050
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=845
PRIMER_PAIR_36_PENALTY=1.056360
PRIMER_LEFT_36_PENALTY=0.004736
PRIMER_RIGHT_36_PENALTY=1.051624
PRIMER_LEFT_36_SEQUENCE=GCTCGCAAGAATCACAAACA
PRIMER_RIGHT_36_SEQUENCE=ATGCGCGAGAGACAAAAAGA
PRIMER_LEFT_36=403,20
PRIMER_RIGHT_36=1219,20
PRIMER_LEFT_36_TM=59.995
PRIMER_RIGHT_36_TM=61.052
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=817
PRIMER_PAIR_37_PENALTY=1.056360
PRIMER_LEFT_37_PENALTY=0.004736
PRIMER_RIGHT_37_PENALTY=1.051624
PRIMER_LEFT_37_SEQUENCE=TGCTCGCAAGAATCACAAAC
PRIMER_RIGHT_37_SEQUENCE=ATGCGCGAGAGACAAAAAGA
PRIMER_LEFT_37=402,20
PRIMER_RIGHT_37=1219,20
PRIMER_LEFT_37_TM=59.995
PRIMER_RIGHT_37_TM=61.052
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=818
PRIMER_PAIR_38_PENALTY=1.061144
PRIMER_LEFT_38_PENALTY=0.306150
PRIMER_RIGHT_38_PENALTY=0.754994
PRIMER_LEFT_38_SEQUENCE=GTGTCGGGGCTTCTTCTATG
PRIMER_RIGHT_38_SEQUENCE=ATTTGGTGCTTTGGTGATGG
PRIMER_LEFT_38=622,20
PRIMER_RIGHT_38=1398,20
PRIMER_LEFT_38_TM=59.694
PRIMER_RIGHT_38_TM=60.755
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.9000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=2.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=777
PRIMER_PAIR_39_PENALTY=1.076917
PRIMER_LEFT_39_PENALTY=0.246726
PRIMER_RIGHT_39_PENALTY=0.830191
PRIMER_LEFT_39_SEQUENCE=AAAGTGTCGGGGCTTCTTCT
PRIMER_RIGHT_39_SEQUENCE=CTTGCTTGATTTGGTTGGTG
PRIMER_LEFT_39=619,20
PRIMER_RIGHT_39=1427,20
PRIMER_LEFT_39_TM=60.247
PRIMER_RIGHT_39_TM=59.170
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=809
PRIMER_PAIR_40_PENALTY=1.097802
PRIMER_LEFT_40_PENALTY=0.575240
PRIMER_RIGHT_40_PENALTY=0.522562
PRIMER_LEFT_40_SEQUENCE=ATGCTCGCAAGAATCACAAA
PRIMER_RIGHT_40_SEQUENCE=AATGCGCGAGAGACAAAAAG
PRIMER_LEFT_40=401,20
PRIMER_RIGHT_40=1220,20
PRIMER_LEFT_40_TM=59.425
PRIMER_RIGHT_40_TM=60.523
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.3000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=820
PRIMER_PAIR_41_PENALTY=1.101470
PRIMER_LEFT_41_PENALTY=0.151533
PRIMER_RIGHT_41_PENALTY=0.949937
PRIMER_LEFT_41_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_41_SEQUENCE=GGCGTGTCTTTATGGTGTTG
PRIMER_LEFT_41=771,20
PRIMER_RIGHT_41=1617,20
PRIMER_LEFT_41_TM=59.848
PRIMER_RIGHT_41_TM=59.050
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=847
PRIMER_PAIR_42_PENALTY=1.111201
PRIMER_LEFT_42_PENALTY=0.088413
PRIMER_RIGHT_42_PENALTY=1.022788
PRIMER_LEFT_42_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_42_SEQUENCE=ACTTGCTTGATTTGGTTGGTG
PRIMER_LEFT_42=773,20
PRIMER_RIGHT_42=1428,21
PRIMER_LEFT_42_TM=60.088
PRIMER_RIGHT_42_TM=60.023
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=656
PRIMER_PAIR_43_PENALTY=1.132575
PRIMER_LEFT_43_PENALTY=0.246726
PRIMER_RIGHT_43_PENALTY=0.885849
PRIMER_LEFT_43_SEQUENCE=AAAGTGTCGGGGCTTCTTCT
PRIMER_RIGHT_43_SEQUENCE=AAATGCGCGAGAGACAAAAA
PRIMER_LEFT_43=619,20
PRIMER_RIGHT_43=1221,20
PRIMER_LEFT_43_TM=60.247
PRIMER_RIGHT_43_TM=60.886
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=603
PRIMER_PAIR_44_PENALTY=1.133199
PRIMER_LEFT_44_PENALTY=0.088413
PRIMER_RIGHT_44_PENALTY=1.044787
PRIMER_LEFT_44_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_44_SEQUENCE=AGGCGTGTCTTTATGGTGTTG
PRIMER_LEFT_44=773,20
PRIMER_RIGHT_44=1618,21
PRIMER_LEFT_44_TM=60.088
PRIMER_RIGHT_44_TM=60.045
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=846
PRIMER_PAIR_45_PENALTY=1.133199
PRIMER_LEFT_45_PENALTY=0.088413
PRIMER_RIGHT_45_PENALTY=1.044787
PRIMER_LEFT_45_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_45_SEQUENCE=CAGGCGTGTCTTTATGGTGTT
PRIMER_LEFT_45=773,20
PRIMER_RIGHT_45=1619,21
PRIMER_LEFT_45_TM=60.088
PRIMER_RIGHT_45_TM=60.045
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=847
PRIMER_PAIR_46_PENALTY=1.136341
PRIMER_LEFT_46_PENALTY=0.306150
PRIMER_RIGHT_46_PENALTY=0.830191
PRIMER_LEFT_46_SEQUENCE=GTGTCGGGGCTTCTTCTATG
PRIMER_RIGHT_46_SEQUENCE=CTTGCTTGATTTGGTTGGTG
PRIMER_LEFT_46=622,20
PRIMER_RIGHT_46=1427,20
PRIMER_LEFT_46_TM=59.694
PRIMER_RIGHT_46_TM=59.170
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=5.9000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=806
PRIMER_PAIR_47_PENALTY=1.140036
PRIMER_LEFT_47_PENALTY=0.088413
PRIMER_RIGHT_47_PENALTY=1.051624
PRIMER_LEFT_47_SEQUENCE=TGGCTAGGCTTTCTTCCTCA
PRIMER_RIGHT_47_SEQUENCE=ATGCGCGAGAGACAAAAAGA
PRIMER_LEFT_47=773,20
PRIMER_RIGHT_47=1219,20
PRIMER_LEFT_47_TM=60.088
PRIMER_RIGHT_47_TM=61.052
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=447
PRIMER_PAIR_48_PENALTY=1.174322
PRIMER_LEFT_48_PENALTY=0.151533
PRIMER_RIGHT_48_PENALTY=1.022788
PRIMER_LEFT_48_SEQUENCE=GGTGGCTAGGCTTTCTTCCT
PRIMER_RIGHT_48_SEQUENCE=ACTTGCTTGATTTGGTTGGTG
PRIMER_LEFT_48=771,20
PRIMER_RIGHT_48=1428,21
PRIMER_LEFT_48_TM=59.848
PRIMER_RIGHT_48_TM=60.023
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=658
PRIMER_PAIR_49_PENALTY=1.191999
PRIMER_LEFT_49_PENALTY=0.306150
PRIMER_RIGHT_49_PENALTY=0.885849
PRIMER_LEFT_49_SEQUENCE=GTGTCGGGGCTTCTTCTATG
PRIMER_RIGHT_49_SEQUENCE=AAATGCGCGAGAGACAAAAA
PRIMER_LEFT_49=622,20
PRIMER_RIGHT_49=1221,20
PRIMER_LEFT_49_TM=59.694
PRIMER_RIGHT_49_TM=60.886
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.9000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=600
=
SEQUENCE_ID=MAGIC.433_4_2840319_2840486_wu-0_oy-0_260355_768839_TRUE
SEQUENCE_TARGET=950,267
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_13891303_13891412_tsu-0_oy-0_88181_80143_TRUE
SEQUENCE_TARGET=950,209
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_3145244_3145352_can-0_wil-2_1140_125598_TRUE
SEQUENCE_TARGET=950,208
SEQUENCE_TEMPLATE=CAANTNCTTTNTAGAATCNAANAGGAAATTNNANACTGTNATTTGCGATAAAGATGAGTGAGAGAAACTNGTTCTTAGATTTNCTTTTATAACGAGAAGTTTCCTTTGGAATCAGAGCAGTTGCATCACGAAGTGGGGANATCCACGACATGGAGATAACCGTCGCNNNNNNNNNNNNNANGAACTGCCCNCATCNTCGACAAAACCGTCNGAGATCNACTCCNGGTAACCGTTANAGCAGATCCAAAANTAANTGTGTATCGCATNCACGATCGCCTTTGCAATCGNNNCCATTNCNTTNNNNNNNATNTGATCCTATGAGATTTNTTTTTTTATATAAAATGATACAAAAGATTAGATTTGAATCTAAATAAACATTTTTCATAAAAACATTTGAGTATNANAANNNCNTCAGCTTTTNNTCTACTGTAAAAAGCTTCTAAAACATTCTCANCCTTCTCCATATTTTNNAGTTCNATGATGATGTATCTTTGTGGAAGANAAACCACCGAACNAGTAATCTNGACGTTAGTACANCTNTNTACCCACAAAGGAGNGAATNCAAAATAGACTACTTTATACTCACNTCNAGANTTAAGATTTGATTGNTNTTCTACTCACACCAAANCAAAAACCAAAGATCATAAAGATCAGATTATTCGTCCTTTNNTCGAANCTTATANATTTATTATTTTATTGANCANTATCTGAAATAANAACAACATNATCANATATTTAACNANAAAAGGTTAATAATAAGGATTNNNNCTGGGGACAAANCCAATCATNCTTGAACGCCATTTCATATGTGTTCTCATTNAAGTANAAANTCGTTACCAATGANAACAAATTTAGCAATGAAACTCATATGGNTGTAATNACNANAAGCAAAATTAATTAAAAACACTTTACCTTCCAAATACAAATAACTCNGAGAGGATCCNCTACCAAGTTCTTCATTGTTTGTTGAAAGAGCNTTTATTGACTCCNNTGGGACACNNTGATGTATNTGTGCNTGGAATGTGTTGAAACATTTTTNNAGAACCTGCAACAAAATTAATATTAGTGATGNTANCTAATTNGCAATAGATAGAANCTAATTCTAGTANAGAATCTTTACTTGAATTCCNNNAACCGATTNTCTTANACANAATCCTTANANAAGTTGTAGAGAATGAAAGATATGCAAGCNTTCTGTTCGAAACATCGAGAATGANCTCATNAGAAAACCCANNCGGTCAAACAGATGCCTGCAANTATTTTACAATCAANCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGAAANTATTNTGTTTCNAAAGTTAATAATATTTTCTNTTGNNACATTTTGCTATGTTTAGGCTATAACACTNTGAAGGNAGATTNTTGAGAAAGGCGGGTTTACGCAGTTTAGACCTCATTATATTACTTGGTATTGTCCTCATACTATCACGTTGAGTCGACAGTNTAAGTCTCANTGTATCAATNAAGGAAGGTATTGTCCTCCTGATCTAGAGCANGACTTTAGCTCGGGATACGATGGAAAAGACGTGGTTATGGAAAATTTGAGACAGCTTTGTGTTTACAAGGTGGCGAATGCAACCGCCACCTTGGGTGTAGTGGNATTATGTTACTGATTTCCAGATCAGATATCCCATGAAGGAGNAAAAAATACAATAAGGAGTGTGCTGAGTCTGTAATCAAATCTCTTGGTGAGTGTTTCTTCAAACATGTCTTGATAGTTAAAAGAAAAGCAATTTTTGGGAGAATGAGGTATTGAGGTCATATGGGAGACCCTAATGTTGACTTGGACAATAATNCAGCTTTAAAGGAAGAACAAGATGCTCAAGTAAATTACTCTTCTCACATCTGAATTTGACTCTTCTTTACGTAACCTGTTTTGGGTTTGACATATTATATTATGTAGGTTGGCAAGGGTANAAGGGGTGATGTTACCATATTGCCTACCTTAGTTGTCAACAANAGACAGTACCGAGGTTTGTTCCGTAGTATTCAACTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.946454
PRIMER_LEFT_0_PENALTY=2.893725
PRIMER_RIGHT_0_PENALTY=0.052729
PRIMER_LEFT_0_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_0_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_0=795,21
PRIMER_RIGHT_0=1630,20
PRIMER_LEFT_0_TM=58.106
PRIMER_RIGHT_0_TM=60.053
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=836
PRIMER_PAIR_1_PENALTY=3.073967
PRIMER_LEFT_1_PENALTY=3.021238
PRIMER_RIGHT_1_PENALTY=0.052729
PRIMER_LEFT_1_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_1_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_1=794,22
PRIMER_RIGHT_1=1630,20
PRIMER_LEFT_1_TM=58.979
PRIMER_RIGHT_1_TM=60.053
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=837
PRIMER_PAIR_2_PENALTY=3.440136
PRIMER_LEFT_2_PENALTY=2.893725
PRIMER_RIGHT_2_PENALTY=0.546410
PRIMER_LEFT_2_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_2_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_2=795,21
PRIMER_RIGHT_2=1643,20
PRIMER_LEFT_2_TM=58.106
PRIMER_RIGHT_2_TM=59.454
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=849
PRIMER_PAIR_3_PENALTY=3.506944
PRIMER_LEFT_3_PENALTY=2.893725
PRIMER_RIGHT_3_PENALTY=0.613219
PRIMER_LEFT_3_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_3_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_3=795,21
PRIMER_RIGHT_3=1638,20
PRIMER_LEFT_3_TM=58.106
PRIMER_RIGHT_3_TM=59.387
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=844
PRIMER_PAIR_4_PENALTY=3.567649
PRIMER_LEFT_4_PENALTY=3.021238
PRIMER_RIGHT_4_PENALTY=0.546410
PRIMER_LEFT_4_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_4_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_4=794,22
PRIMER_RIGHT_4=1643,20
PRIMER_LEFT_4_TM=58.979
PRIMER_RIGHT_4_TM=59.454
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=850
PRIMER_PAIR_5_PENALTY=3.634457
PRIMER_LEFT_5_PENALTY=3.021238
PRIMER_RIGHT_5_PENALTY=0.613219
PRIMER_LEFT_5_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_5_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_5=794,22
PRIMER_RIGHT_5=1638,20
PRIMER_LEFT_5_TM=58.979
PRIMER_RIGHT_5_TM=59.387
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=9.3000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=845
PRIMER_PAIR_6_PENALTY=3.925545
PRIMER_LEFT_6_PENALTY=3.872816
PRIMER_RIGHT_6_PENALTY=0.052729
PRIMER_LEFT_6_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_6_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_6=795,20
PRIMER_RIGHT_6=1630,20
PRIMER_LEFT_6_TM=56.127
PRIMER_RIGHT_6_TM=60.053
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=836
PRIMER_PAIR_7_PENALTY=4.094803
PRIMER_LEFT_7_PENALTY=2.893725
PRIMER_RIGHT_7_PENALTY=1.201078
PRIMER_LEFT_7_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_7_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_7=795,21
PRIMER_RIGHT_7=1639,21
PRIMER_LEFT_7_TM=58.106
PRIMER_RIGHT_7_TM=60.201
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=9.3000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=845
PRIMER_PAIR_8_PENALTY=4.097174
PRIMER_LEFT_8_PENALTY=4.044445
PRIMER_RIGHT_8_PENALTY=0.052729
PRIMER_LEFT_8_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_8_SEQUENCE=CCATCGTATCCCGAGCTAAA
PRIMER_LEFT_8=794,20
PRIMER_RIGHT_8=1630,20
PRIMER_LEFT_8_TM=55.956
PRIMER_RIGHT_8_TM=60.053
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.3000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=837
PRIMER_PAIR_9_PENALTY=4.222316
PRIMER_LEFT_9_PENALTY=3.021238
PRIMER_RIGHT_9_PENALTY=1.201078
PRIMER_LEFT_9_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_9_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_9=794,22
PRIMER_RIGHT_9=1639,21
PRIMER_LEFT_9_TM=58.979
PRIMER_RIGHT_9_TM=60.201
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=9.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=846
PRIMER_PAIR_10_PENALTY=4.244156
PRIMER_LEFT_10_PENALTY=2.893725
PRIMER_RIGHT_10_PENALTY=1.350430
PRIMER_LEFT_10_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_10_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_10=795,21
PRIMER_RIGHT_10=1648,21
PRIMER_LEFT_10_TM=58.106
PRIMER_RIGHT_10_TM=60.350
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=854
PRIMER_PAIR_11_PENALTY=4.371669
PRIMER_LEFT_11_PENALTY=3.021238
PRIMER_RIGHT_11_PENALTY=1.350430
PRIMER_LEFT_11_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_11_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_11=794,22
PRIMER_RIGHT_11=1648,21
PRIMER_LEFT_11_TM=58.979
PRIMER_RIGHT_11_TM=60.350
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=855
PRIMER_PAIR_12_PENALTY=4.408927
PRIMER_LEFT_12_PENALTY=2.893725
PRIMER_RIGHT_12_PENALTY=1.515201
PRIMER_LEFT_12_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_12_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_12=795,21
PRIMER_RIGHT_12=1644,21
PRIMER_LEFT_12_TM=58.106
PRIMER_RIGHT_12_TM=59.485
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=850
PRIMER_PAIR_13_PENALTY=4.419194
PRIMER_LEFT_13_PENALTY=2.893725
PRIMER_RIGHT_13_PENALTY=1.525468
PRIMER_LEFT_13_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_13_SEQUENCE=CCATAACCACGTCTTTTCCA
PRIMER_LEFT_13=795,21
PRIMER_RIGHT_13=1647,20
PRIMER_LEFT_13_TM=58.106
PRIMER_RIGHT_13_TM=58.475
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=853
PRIMER_PAIR_14_PENALTY=4.419226
PRIMER_LEFT_14_PENALTY=3.872816
PRIMER_RIGHT_14_PENALTY=0.546410
PRIMER_LEFT_14_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_14_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_14=795,20
PRIMER_RIGHT_14=1643,20
PRIMER_LEFT_14_TM=56.127
PRIMER_RIGHT_14_TM=59.454
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=849
PRIMER_PAIR_15_PENALTY=4.450494
PRIMER_LEFT_15_PENALTY=2.893725
PRIMER_RIGHT_15_PENALTY=1.556768
PRIMER_LEFT_15_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_15_SEQUENCE=CCACGTCTTTTCCATCGTATC
PRIMER_LEFT_15=795,21
PRIMER_RIGHT_15=1641,21
PRIMER_LEFT_15_TM=58.106
PRIMER_RIGHT_15_TM=59.443
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=5.3000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=847
PRIMER_PAIR_16_PENALTY=4.486035
PRIMER_LEFT_16_PENALTY=3.872816
PRIMER_RIGHT_16_PENALTY=0.613219
PRIMER_LEFT_16_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_16_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_16=795,20
PRIMER_RIGHT_16=1638,20
PRIMER_LEFT_16_TM=56.127
PRIMER_RIGHT_16_TM=59.387
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=9.3000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=844
PRIMER_PAIR_17_PENALTY=4.536440
PRIMER_LEFT_17_PENALTY=3.021238
PRIMER_RIGHT_17_PENALTY=1.515201
PRIMER_LEFT_17_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_17_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_17=794,22
PRIMER_RIGHT_17=1644,21
PRIMER_LEFT_17_TM=58.979
PRIMER_RIGHT_17_TM=59.485
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=851
PRIMER_PAIR_18_PENALTY=4.546707
PRIMER_LEFT_18_PENALTY=3.021238
PRIMER_RIGHT_18_PENALTY=1.525468
PRIMER_LEFT_18_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_18_SEQUENCE=CCATAACCACGTCTTTTCCA
PRIMER_LEFT_18=794,22
PRIMER_RIGHT_18=1647,20
PRIMER_LEFT_18_TM=58.979
PRIMER_RIGHT_18_TM=58.475
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=854
PRIMER_PAIR_19_PENALTY=4.578007
PRIMER_LEFT_19_PENALTY=3.021238
PRIMER_RIGHT_19_PENALTY=1.556768
PRIMER_LEFT_19_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_19_SEQUENCE=CCACGTCTTTTCCATCGTATC
PRIMER_LEFT_19=794,22
PRIMER_RIGHT_19=1641,21
PRIMER_LEFT_19_TM=58.979
PRIMER_RIGHT_19_TM=59.443
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=5.3000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=848
PRIMER_PAIR_20_PENALTY=4.590856
PRIMER_LEFT_20_PENALTY=4.044445
PRIMER_RIGHT_20_PENALTY=0.546410
PRIMER_LEFT_20_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_20_SEQUENCE=AACCACGTCTTTTCCATCGT
PRIMER_LEFT_20=794,20
PRIMER_RIGHT_20=1643,20
PRIMER_LEFT_20_TM=55.956
PRIMER_RIGHT_20_TM=59.454
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=8.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=850
PRIMER_PAIR_21_PENALTY=4.657664
PRIMER_LEFT_21_PENALTY=4.044445
PRIMER_RIGHT_21_PENALTY=0.613219
PRIMER_LEFT_21_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_21_SEQUENCE=CGTCTTTTCCATCGTATCCC
PRIMER_LEFT_21=794,20
PRIMER_RIGHT_21=1638,20
PRIMER_LEFT_21_TM=55.956
PRIMER_RIGHT_21_TM=59.387
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.3000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=845
PRIMER_PAIR_22_PENALTY=4.745773
PRIMER_LEFT_22_PENALTY=2.893725
PRIMER_RIGHT_22_PENALTY=1.852047
PRIMER_LEFT_22_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_22_SEQUENCE=TAAACTGCGTAAACCCGCCT
PRIMER_LEFT_22=795,21
PRIMER_RIGHT_22=1500,20
PRIMER_LEFT_22_TM=58.106
PRIMER_RIGHT_22_TM=61.852
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=11.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=706
PRIMER_PAIR_23_PENALTY=4.745773
PRIMER_LEFT_23_PENALTY=2.893725
PRIMER_RIGHT_23_PENALTY=1.852047
PRIMER_LEFT_23_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_23_SEQUENCE=CTAAACTGCGTAAACCCGCC
PRIMER_LEFT_23=795,21
PRIMER_RIGHT_23=1501,20
PRIMER_LEFT_23_TM=58.106
PRIMER_RIGHT_23_TM=61.852
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=12.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=707
PRIMER_PAIR_24_PENALTY=4.873286
PRIMER_LEFT_24_PENALTY=3.021238
PRIMER_RIGHT_24_PENALTY=1.852047
PRIMER_LEFT_24_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_24_SEQUENCE=TAAACTGCGTAAACCCGCCT
PRIMER_LEFT_24=794,22
PRIMER_RIGHT_24=1500,20
PRIMER_LEFT_24_TM=58.979
PRIMER_RIGHT_24_TM=61.852
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=11.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=707
PRIMER_PAIR_25_PENALTY=4.873286
PRIMER_LEFT_25_PENALTY=3.021238
PRIMER_RIGHT_25_PENALTY=1.852047
PRIMER_LEFT_25_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_25_SEQUENCE=CTAAACTGCGTAAACCCGCC
PRIMER_LEFT_25=794,22
PRIMER_RIGHT_25=1501,20
PRIMER_LEFT_25_TM=58.979
PRIMER_RIGHT_25_TM=61.852
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=12.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=708
PRIMER_PAIR_26_PENALTY=4.955357
PRIMER_LEFT_26_PENALTY=2.893725
PRIMER_RIGHT_26_PENALTY=2.061632
PRIMER_LEFT_26_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_26_SEQUENCE=TTCCATAACCACGTCTTTTCC
PRIMER_LEFT_26=795,21
PRIMER_RIGHT_26=1649,21
PRIMER_LEFT_26_TM=58.106
PRIMER_RIGHT_26_TM=58.938
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=855
PRIMER_PAIR_27_PENALTY=5.073893
PRIMER_LEFT_27_PENALTY=3.872816
PRIMER_RIGHT_27_PENALTY=1.201078
PRIMER_LEFT_27_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_27_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_27=795,20
PRIMER_RIGHT_27=1639,21
PRIMER_LEFT_27_TM=56.127
PRIMER_RIGHT_27_TM=60.201
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=9.3000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=845
PRIMER_PAIR_28_PENALTY=5.082870
PRIMER_LEFT_28_PENALTY=3.021238
PRIMER_RIGHT_28_PENALTY=2.061632
PRIMER_LEFT_28_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_28_SEQUENCE=TTCCATAACCACGTCTTTTCC
PRIMER_LEFT_28=794,22
PRIMER_RIGHT_28=1649,21
PRIMER_LEFT_28_TM=58.979
PRIMER_RIGHT_28_TM=58.938
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=856
PRIMER_PAIR_29_PENALTY=5.085291
PRIMER_LEFT_29_PENALTY=2.893725
PRIMER_RIGHT_29_PENALTY=2.191566
PRIMER_LEFT_29_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_29_SEQUENCE=TTGCAGGCATCTGTTTGACC
PRIMER_LEFT_29=795,21
PRIMER_RIGHT_29=1255,20
PRIMER_LEFT_29_TM=58.106
PRIMER_RIGHT_29_TM=62.192
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=461
PRIMER_PAIR_30_PENALTY=5.110525
PRIMER_LEFT_30_PENALTY=2.893725
PRIMER_RIGHT_30_PENALTY=2.216800
PRIMER_LEFT_30_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_30_SEQUENCE=TTTCCATAACCACGTCTTTTCC
PRIMER_LEFT_30=795,21
PRIMER_RIGHT_30=1650,22
PRIMER_LEFT_30_TM=58.106
PRIMER_RIGHT_30_TM=60.217
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=856
PRIMER_PAIR_31_PENALTY=5.125785
PRIMER_LEFT_31_PENALTY=2.893725
PRIMER_RIGHT_31_PENALTY=2.232060
PRIMER_LEFT_31_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_31_SEQUENCE=ATAACCACGTCTTTTCCATCGT
PRIMER_LEFT_31=795,21
PRIMER_RIGHT_31=1645,22
PRIMER_LEFT_31_TM=58.106
PRIMER_RIGHT_31_TM=59.768
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=8.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=851
PRIMER_PAIR_32_PENALTY=5.142799
PRIMER_LEFT_32_PENALTY=2.893725
PRIMER_RIGHT_32_PENALTY=2.249074
PRIMER_LEFT_32_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_32_SEQUENCE=ACCACGTCTTTTCCATCGTATC
PRIMER_LEFT_32=795,21
PRIMER_RIGHT_32=1642,22
PRIMER_LEFT_32_TM=58.106
PRIMER_RIGHT_32_TM=60.249
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=5.3000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=848
PRIMER_PAIR_33_PENALTY=5.212804
PRIMER_LEFT_33_PENALTY=3.021238
PRIMER_RIGHT_33_PENALTY=2.191566
PRIMER_LEFT_33_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_33_SEQUENCE=TTGCAGGCATCTGTTTGACC
PRIMER_LEFT_33=794,22
PRIMER_RIGHT_33=1255,20
PRIMER_LEFT_33_TM=58.979
PRIMER_RIGHT_33_TM=62.192
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=462
PRIMER_PAIR_34_PENALTY=5.223246
PRIMER_LEFT_34_PENALTY=3.872816
PRIMER_RIGHT_34_PENALTY=1.350430
PRIMER_LEFT_34_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_34_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_34=795,20
PRIMER_RIGHT_34=1648,21
PRIMER_LEFT_34_TM=56.127
PRIMER_RIGHT_34_TM=60.350
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=854
PRIMER_PAIR_35_PENALTY=5.238038
PRIMER_LEFT_35_PENALTY=3.021238
PRIMER_RIGHT_35_PENALTY=2.216800
PRIMER_LEFT_35_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_35_SEQUENCE=TTTCCATAACCACGTCTTTTCC
PRIMER_LEFT_35=794,22
PRIMER_RIGHT_35=1650,22
PRIMER_LEFT_35_TM=58.979
PRIMER_RIGHT_35_TM=60.217
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=857
PRIMER_PAIR_36_PENALTY=5.244263
PRIMER_LEFT_36_PENALTY=2.893725
PRIMER_RIGHT_36_PENALTY=2.350537
PRIMER_LEFT_36_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_36_SEQUENCE=CGTAAACCCGCCTTTCTCAA
PRIMER_LEFT_36=795,21
PRIMER_RIGHT_36=1493,20
PRIMER_LEFT_36_TM=58.106
PRIMER_RIGHT_36_TM=62.351
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=699
PRIMER_PAIR_37_PENALTY=5.245523
PRIMER_LEFT_37_PENALTY=4.044445
PRIMER_RIGHT_37_PENALTY=1.201078
PRIMER_LEFT_37_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_37_SEQUENCE=ACGTCTTTTCCATCGTATCCC
PRIMER_LEFT_37=794,20
PRIMER_RIGHT_37=1639,21
PRIMER_LEFT_37_TM=55.956
PRIMER_RIGHT_37_TM=60.201
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=9.3000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=846
PRIMER_PAIR_38_PENALTY=5.253298
PRIMER_LEFT_38_PENALTY=3.021238
PRIMER_RIGHT_38_PENALTY=2.232060
PRIMER_LEFT_38_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_38_SEQUENCE=ATAACCACGTCTTTTCCATCGT
PRIMER_LEFT_38=794,22
PRIMER_RIGHT_38=1645,22
PRIMER_LEFT_38_TM=58.979
PRIMER_RIGHT_38_TM=59.768
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=8.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=852
PRIMER_PAIR_39_PENALTY=5.270312
PRIMER_LEFT_39_PENALTY=3.021238
PRIMER_RIGHT_39_PENALTY=2.249074
PRIMER_LEFT_39_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_39_SEQUENCE=ACCACGTCTTTTCCATCGTATC
PRIMER_LEFT_39=794,22
PRIMER_RIGHT_39=1642,22
PRIMER_LEFT_39_TM=58.979
PRIMER_RIGHT_39_TM=60.249
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=5.3000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=849
PRIMER_PAIR_40_PENALTY=5.371776
PRIMER_LEFT_40_PENALTY=3.021238
PRIMER_RIGHT_40_PENALTY=2.350537
PRIMER_LEFT_40_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_40_SEQUENCE=CGTAAACCCGCCTTTCTCAA
PRIMER_LEFT_40=794,22
PRIMER_RIGHT_40=1493,20
PRIMER_LEFT_40_TM=58.979
PRIMER_RIGHT_40_TM=62.351
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=700
PRIMER_PAIR_41_PENALTY=5.376379
PRIMER_LEFT_41_PENALTY=2.893725
PRIMER_RIGHT_41_PENALTY=2.482654
PRIMER_LEFT_41_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_41_SEQUENCE=TCCATAACCACGTCTTTTCC
PRIMER_LEFT_41=795,21
PRIMER_RIGHT_41=1648,20
PRIMER_LEFT_41_TM=58.106
PRIMER_RIGHT_41_TM=57.517
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=854
PRIMER_PAIR_42_PENALTY=5.388017
PRIMER_LEFT_42_PENALTY=3.872816
PRIMER_RIGHT_42_PENALTY=1.515201
PRIMER_LEFT_42_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_42_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_42=795,20
PRIMER_RIGHT_42=1644,21
PRIMER_LEFT_42_TM=56.127
PRIMER_RIGHT_42_TM=59.485
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=8.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=850
PRIMER_PAIR_43_PENALTY=5.394876
PRIMER_LEFT_43_PENALTY=4.044445
PRIMER_RIGHT_43_PENALTY=1.350430
PRIMER_LEFT_43_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_43_SEQUENCE=TCCATAACCACGTCTTTTCCA
PRIMER_LEFT_43=794,20
PRIMER_RIGHT_43=1648,21
PRIMER_LEFT_43_TM=55.956
PRIMER_RIGHT_43_TM=60.350
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=855
PRIMER_PAIR_44_PENALTY=5.398284
PRIMER_LEFT_44_PENALTY=3.872816
PRIMER_RIGHT_44_PENALTY=1.525468
PRIMER_LEFT_44_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_44_SEQUENCE=CCATAACCACGTCTTTTCCA
PRIMER_LEFT_44=795,20
PRIMER_RIGHT_44=1647,20
PRIMER_LEFT_44_TM=56.127
PRIMER_RIGHT_44_TM=58.475
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=853
PRIMER_PAIR_45_PENALTY=5.429584
PRIMER_LEFT_45_PENALTY=3.872816
PRIMER_RIGHT_45_PENALTY=1.556768
PRIMER_LEFT_45_SEQUENCE=CGCCATTTCATATGTGTTCT
PRIMER_RIGHT_45_SEQUENCE=CCACGTCTTTTCCATCGTATC
PRIMER_LEFT_45=795,20
PRIMER_RIGHT_45=1641,21
PRIMER_LEFT_45_TM=56.127
PRIMER_RIGHT_45_TM=59.443
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=5.3000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=847
PRIMER_PAIR_46_PENALTY=5.486405
PRIMER_LEFT_46_PENALTY=2.893725
PRIMER_RIGHT_46_PENALTY=2.592680
PRIMER_LEFT_46_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_46_SEQUENCE=CCATAACCACGTCTTTTCCATC
PRIMER_LEFT_46=795,21
PRIMER_RIGHT_46=1647,22
PRIMER_LEFT_46_TM=58.106
PRIMER_RIGHT_46_TM=60.593
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=853
PRIMER_PAIR_47_PENALTY=5.503892
PRIMER_LEFT_47_PENALTY=3.021238
PRIMER_RIGHT_47_PENALTY=2.482654
PRIMER_LEFT_47_SEQUENCE=ACGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_47_SEQUENCE=TCCATAACCACGTCTTTTCC
PRIMER_LEFT_47=794,22
PRIMER_RIGHT_47=1648,20
PRIMER_LEFT_47_TM=58.979
PRIMER_RIGHT_47_TM=57.517
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=855
PRIMER_PAIR_48_PENALTY=5.528062
PRIMER_LEFT_48_PENALTY=2.893725
PRIMER_RIGHT_48_PENALTY=2.634337
PRIMER_LEFT_48_SEQUENCE=CGCCATTTCATATGTGTTCTC
PRIMER_RIGHT_48_SEQUENCE=CATCGTATCCCGAGCTAAAGT
PRIMER_LEFT_48=795,21
PRIMER_RIGHT_48=1629,21
PRIMER_LEFT_48_TM=58.106
PRIMER_RIGHT_48_TM=58.366
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=835
PRIMER_PAIR_49_PENALTY=5.559647
PRIMER_LEFT_49_PENALTY=4.044445
PRIMER_RIGHT_49_PENALTY=1.515201
PRIMER_LEFT_49_SEQUENCE=ACGCCATTTCATATGTGTTC
PRIMER_RIGHT_49_SEQUENCE=TAACCACGTCTTTTCCATCGT
PRIMER_LEFT_49=794,20
PRIMER_RIGHT_49=1644,21
PRIMER_LEFT_49_TM=55.956
PRIMER_RIGHT_49_TM=59.485
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=851
=
SEQUENCE_ID=MAGIC.149_4_4449285_4449574_wil-2_edi-0_3111_31780_TRUE
SEQUENCE_TARGET=950,389
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTCGATATTATTCACNAAATCTTGNGTTTTACTTNGAGNTAACTCCAAATCTNATGAAAACCCTAGNACTAAGCTAAANNCTACTNTATATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNACACATTTTCTTCCCACATNNNTAAATTTGAATTTATTANAGNTANNCACCANAAGCAAACNATGCCAAGANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTACATTTGATGAAGTAGCTGATCNTTTTACTTNGANCAGAAAANNNNAAAAGTTCCAACTAAGGNAAAGATCTNCCAAAATTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTNTCNNAGNTCCAAAAAATNACAAAGAGNTGAGGACGTGNGATGGTGTTGTGTATGAGACNTACACTGAAGTNTGTGCNGNNCGAGNATTTATGTAANAAGAGNGNNNNNANNCCNTNNNNNNNAANNTNTNCNATTCAATTTNCTTTAAGTTCTATNTNTTTATTTTCAATTNCTNCTTTGTTATTCANACNNNNNNNTAATTCATGTTTNANTTATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17337671_17337696_ct-1_po-0_36356_69855_TRUE
SEQUENCE_TARGET=950,125
SEQUENCE_TEMPLATE=CTGTTTTTGAACAGAACAGGAACGTTTTTGGGGGTTTCGATATCATTGATTCTTATAAACTTAGCTGCGATCATGGAACGTGCAGACGAGAATCTATTGCCTTCGGTTTATAAAGAAGTGAGTGAAGCATTTAACGCAGGACCATCAGATTTAGGCTACTTAACATTCGTTAGAAACTTTGTTCAAGGACTTGCATCACCATTAGCAGGAGTTCTTGTCATTACCTATGATCGTCCCATTGTTCTTGCAATAGGTACTNTCTGTTGGGCTTTATCAACTGCTGCAGTTGGAGCCAGCAGCTACTTCATTCAGGTACTTTTTGATCTGTTTCTTTAAGGACTTTGATTTTGAAGTTATAAGTTTTTGAATGGTCTTGTGATATAGGTTGCTTTATGGAGAGCAGTGAATGGTTTTGGATTGGCAATTGTTATACCCGCGCTTCAATCGTTTATTGCAGATAGTTATAAGGATGGTGCGAGAGGAGCTGGTTTCGGAATGTTGAACCTCATTGGTACAATCGGTGGTATAGGAGGAGGTGTTGTAGCAACTGTTATGGCTGGNTCAGAGTTTTGGGGCATACCGGGATGGCGTTGTGCTTTTATAATGATGGCAGCGCTCAGCGCAGTGATCGGATTACTTGTCTTTCTCTTCGTTGTTGACCCGAGAAAGAACATTGAACGGTAAACAAAACACTCAAGAATTGTACCCTGAATTTTAAGAGTAAGACCAAAGTTTGATGAGGTTTTCTGNTTGTAATTGTAGAGAGGAACTAATGGCTCATAAGATGAATTCGAACTCGGTTTGGAATGATTCATTAGCAGCTGCAAAATCTGTCGTCAAAGTAAGTACCTTTCAGATAATCGTCGCGCAAGGAATCATTGGTTCGTTTCCGTGGACCGCGATGGTTTTCTTTACAATGTGGTTTGAGCTTATTGGTAAACGGCATTTNCTACAAAAAACACTTTGAACATCCATTGTTTGTCAAGAACTGAGATCTTGANTGTAAACAGTCTTTTTTTTTNTTTNTTTTAGGCTTCGATCATAACCAGACTGCAGCTTTGCTTGGGGTATTTGCTACAGGAGGAGCGATAGGAACATTAATGGGAGGGATAATAGCNGATAAAATGTCGCGGATATATCCGAATTCGGGTAGNGTGATGTGTGCGCAGTTCAGTGCATTCATGGGAATCCCATTCTCTATTATTCTTCTGAAAGTAATCCCACAAAGCACAAGCAGCTACTCAATCTTCTCGATAACTCTCTTCCTGATGGGTCTTACCATAACTTGGTGCGGATCAGCGGTNAATGCACCNATGTTTGCAGAAGTNGTTCCTCCAAGGCACCGTACAATGATCTACGCGTTTGACCGTGCTTTCGAAGGGTCATTCTCATCTTTTGCTGCGCCTTTGGTNGGAATTTTGTCNGAGAAANTGTTTGGGTATGACTCAAGAGGTATTGATCCTTTGAAAGGTTCCTCTGTTCGTGAGGCTGATGCCCTTTCAAAGGGGCTTCTGTCAATGATGGCTGTTCCGTTTGGGCTCTGTTGTCTCTGTTACACTCCGTTGCATTTTGTTTTCCAGAAAGATCGAGAAAACGCGAAAATCGCGAGCTCTAAAGAAACTGAAATGATCTGAGAGACTTGCTCATGTAGTCATGTTNTGATTCTTTTTATACCACAAACGATTTTAAAACCACTATGTGAAGCAAAGGTGGACTTTTAACCGAGACCAACNGAATTTGAAAGCTGAAAACGCTTTTTGTTCTGATCAAACCGAATAAATCAAAACGTATGTATATATGATTTGAAATTTTGAATCTAAACCTGAAACTTGAATAAACTTGGAACACGTGACCGNAATTGCGGTTAGAAAAATAAGACCGTTGTAAACGGAGCGTAATCGTGATCAATTTCCNTTTTTCTTACAAGTCCAATCATGAAANATGGAAAATAATAATCTAATGTTTCTTTTTTTTGCGTGAACCTCTCTTTATAAACATTCATCTTTTCTTCTTCTAAACCCTAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.541919
PRIMER_LEFT_0_PENALTY=0.347318
PRIMER_RIGHT_0_PENALTY=0.194601
PRIMER_LEFT_0_SEQUENCE=ATAAGGATGGTGCGAGAGGA
PRIMER_RIGHT_0_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_0=463,20
PRIMER_RIGHT_0=1239,20
PRIMER_LEFT_0_TM=59.653
PRIMER_RIGHT_0_TM=60.195
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=777
PRIMER_PAIR_1_PENALTY=0.547724
PRIMER_LEFT_1_PENALTY=0.353123
PRIMER_RIGHT_1_PENALTY=0.194601
PRIMER_LEFT_1_SEQUENCE=TAAGGATGGTGCGAGAGGAG
PRIMER_RIGHT_1_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_1=464,20
PRIMER_RIGHT_1=1239,20
PRIMER_LEFT_1_TM=60.353
PRIMER_RIGHT_1_TM=60.195
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=776
PRIMER_PAIR_2_PENALTY=0.592980
PRIMER_LEFT_2_PENALTY=0.239857
PRIMER_RIGHT_2_PENALTY=0.353123
PRIMER_LEFT_2_SEQUENCE=TTCAAGGACTTGCATCACCA
PRIMER_RIGHT_2_SEQUENCE=TGTTCCTATCGCTCCTCCTG
PRIMER_LEFT_2=181,20
PRIMER_RIGHT_2=1096,20
PRIMER_LEFT_2_TM=60.240
PRIMER_RIGHT_2_TM=60.353
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=916
PRIMER_PAIR_3_PENALTY=0.670977
PRIMER_LEFT_3_PENALTY=0.476376
PRIMER_RIGHT_3_PENALTY=0.194601
PRIMER_LEFT_3_SEQUENCE=ATTGTTATACCCGCGCTTCA
PRIMER_RIGHT_3_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_3=423,20
PRIMER_RIGHT_3=1239,20
PRIMER_LEFT_3_TM=60.476
PRIMER_RIGHT_3_TM=60.195
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=817
PRIMER_PAIR_4_PENALTY=0.675323
PRIMER_LEFT_4_PENALTY=0.177056
PRIMER_RIGHT_4_PENALTY=0.498267
PRIMER_LEFT_4_SEQUENCE=TGCATCACCATTAGCAGGAG
PRIMER_RIGHT_4_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_4=191,20
PRIMER_RIGHT_4=1187,20
PRIMER_LEFT_4_TM=59.823
PRIMER_RIGHT_4_TM=59.502
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=997
PRIMER_PAIR_5_PENALTY=0.718121
PRIMER_LEFT_5_PENALTY=0.219854
PRIMER_RIGHT_5_PENALTY=0.498267
PRIMER_LEFT_5_SEQUENCE=TTGCATCACCATTAGCAGGA
PRIMER_RIGHT_5_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_5=190,20
PRIMER_RIGHT_5=1187,20
PRIMER_LEFT_5_TM=60.220
PRIMER_RIGHT_5_TM=59.502
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=998
PRIMER_PAIR_6_PENALTY=0.747655
PRIMER_LEFT_6_PENALTY=0.707509
PRIMER_RIGHT_6_PENALTY=0.040146
PRIMER_LEFT_6_SEQUENCE=AATCATTGGTTCGTTTCCGT
PRIMER_RIGHT_6_SEQUENCE=TTCGCGTTTTCTCGATCTTT
PRIMER_LEFT_6=873,20
PRIMER_RIGHT_6=1599,20
PRIMER_LEFT_6_TM=59.292
PRIMER_RIGHT_6_TM=59.960
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=727
PRIMER_PAIR_7_PENALTY=0.747655
PRIMER_LEFT_7_PENALTY=0.707509
PRIMER_RIGHT_7_PENALTY=0.040146
PRIMER_LEFT_7_SEQUENCE=AATCATTGGTTCGTTTCCGT
PRIMER_RIGHT_7_SEQUENCE=TTTCGCGTTTTCTCGATCTT
PRIMER_LEFT_7=873,20
PRIMER_RIGHT_7=1600,20
PRIMER_LEFT_7_TM=59.292
PRIMER_RIGHT_7_TM=59.960
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=728
PRIMER_PAIR_8_PENALTY=0.769956
PRIMER_LEFT_8_PENALTY=0.575355
PRIMER_RIGHT_8_PENALTY=0.194601
PRIMER_LEFT_8_SEQUENCE=CGGTGGTATAGGAGGAGGTG
PRIMER_RIGHT_8_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_8=518,20
PRIMER_RIGHT_8=1239,20
PRIMER_LEFT_8_TM=59.425
PRIMER_RIGHT_8_TM=60.195
PRIMER_LEFT_8_GC_PERCENT=60.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=722
PRIMER_PAIR_9_PENALTY=0.777763
PRIMER_LEFT_9_PENALTY=0.737617
PRIMER_RIGHT_9_PENALTY=0.040146
PRIMER_LEFT_9_SEQUENCE=TGTTGACCCGAGAAAGAACA
PRIMER_RIGHT_9_SEQUENCE=TTTCGCGTTTTCTCGATCTT
PRIMER_LEFT_9=653,20
PRIMER_RIGHT_9=1600,20
PRIMER_LEFT_9_TM=59.262
PRIMER_RIGHT_9_TM=59.960
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=948
PRIMER_PAIR_10_PENALTY=0.780930
PRIMER_LEFT_10_PENALTY=0.347318
PRIMER_RIGHT_10_PENALTY=0.433612
PRIMER_LEFT_10_SEQUENCE=ATAAGGATGGTGCGAGAGGA
PRIMER_RIGHT_10_SEQUENCE=CGCAGCAAAAGATGAGAATG
PRIMER_LEFT_10=463,20
PRIMER_RIGHT_10=1402,20
PRIMER_LEFT_10_TM=59.653
PRIMER_RIGHT_10_TM=59.566
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=940
PRIMER_PAIR_11_PENALTY=0.786735
PRIMER_LEFT_11_PENALTY=0.353123
PRIMER_RIGHT_11_PENALTY=0.433612
PRIMER_LEFT_11_SEQUENCE=TAAGGATGGTGCGAGAGGAG
PRIMER_RIGHT_11_SEQUENCE=CGCAGCAAAAGATGAGAATG
PRIMER_LEFT_11=464,20
PRIMER_RIGHT_11=1402,20
PRIMER_LEFT_11_TM=60.353
PRIMER_RIGHT_11_TM=59.566
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=939
PRIMER_PAIR_12_PENALTY=0.795140
PRIMER_LEFT_12_PENALTY=0.754994
PRIMER_RIGHT_12_PENALTY=0.040146
PRIMER_LEFT_12_SEQUENCE=ATCATTGGTTCGTTTCCGTG
PRIMER_RIGHT_12_SEQUENCE=TTCGCGTTTTCTCGATCTTT
PRIMER_LEFT_12=874,20
PRIMER_RIGHT_12=1599,20
PRIMER_LEFT_12_TM=60.755
PRIMER_RIGHT_12_TM=59.960
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=726
PRIMER_PAIR_13_PENALTY=0.795140
PRIMER_LEFT_13_PENALTY=0.754994
PRIMER_RIGHT_13_PENALTY=0.040146
PRIMER_LEFT_13_SEQUENCE=ATCATTGGTTCGTTTCCGTG
PRIMER_RIGHT_13_SEQUENCE=TTTCGCGTTTTCTCGATCTT
PRIMER_LEFT_13=874,20
PRIMER_RIGHT_13=1600,20
PRIMER_LEFT_13_TM=60.755
PRIMER_RIGHT_13_TM=59.960
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=727
PRIMER_PAIR_14_PENALTY=0.810845
PRIMER_LEFT_14_PENALTY=0.457721
PRIMER_RIGHT_14_PENALTY=0.353123
PRIMER_LEFT_14_SEQUENCE=CATTTAACGCAGGACCATCA
PRIMER_RIGHT_14_SEQUENCE=TGTTCCTATCGCTCCTCCTG
PRIMER_LEFT_14=127,20
PRIMER_RIGHT_14=1096,20
PRIMER_LEFT_14_TM=59.542
PRIMER_RIGHT_14_TM=60.353
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=970
PRIMER_PAIR_15_PENALTY=0.829500
PRIMER_LEFT_15_PENALTY=0.476376
PRIMER_RIGHT_15_PENALTY=0.353123
PRIMER_LEFT_15_SEQUENCE=ATTGTTATACCCGCGCTTCA
PRIMER_RIGHT_15_SEQUENCE=TGTTCCTATCGCTCCTCCTG
PRIMER_LEFT_15=423,20
PRIMER_RIGHT_15=1096,20
PRIMER_LEFT_15_TM=60.476
PRIMER_RIGHT_15_TM=60.353
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=674
PRIMER_PAIR_16_PENALTY=0.830633
PRIMER_LEFT_16_PENALTY=0.636033
PRIMER_RIGHT_16_PENALTY=0.194601
PRIMER_LEFT_16_SEQUENCE=AGCTGGTTTCGGAATGTTGA
PRIMER_RIGHT_16_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_16=482,20
PRIMER_RIGHT_16=1239,20
PRIMER_LEFT_16_TM=60.636
PRIMER_RIGHT_16_TM=60.195
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=758
PRIMER_PAIR_17_PENALTY=0.830633
PRIMER_LEFT_17_PENALTY=0.636033
PRIMER_RIGHT_17_PENALTY=0.194601
PRIMER_LEFT_17_SEQUENCE=GAGCTGGTTTCGGAATGTTG
PRIMER_RIGHT_17_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_17=481,20
PRIMER_RIGHT_17=1239,20
PRIMER_LEFT_17_TM=60.636
PRIMER_RIGHT_17_TM=60.195
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=759
PRIMER_PAIR_18_PENALTY=0.845585
PRIMER_LEFT_18_PENALTY=0.347318
PRIMER_RIGHT_18_PENALTY=0.498267
PRIMER_LEFT_18_SEQUENCE=ATAAGGATGGTGCGAGAGGA
PRIMER_RIGHT_18_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_18=463,20
PRIMER_RIGHT_18=1187,20
PRIMER_LEFT_18_TM=59.653
PRIMER_RIGHT_18_TM=59.502
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=725
PRIMER_PAIR_19_PENALTY=0.851390
PRIMER_LEFT_19_PENALTY=0.353123
PRIMER_RIGHT_19_PENALTY=0.498267
PRIMER_LEFT_19_SEQUENCE=TAAGGATGGTGCGAGAGGAG
PRIMER_RIGHT_19_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_19=464,20
PRIMER_RIGHT_19=1187,20
PRIMER_LEFT_19_TM=60.353
PRIMER_RIGHT_19_TM=59.502
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=724
PRIMER_PAIR_20_PENALTY=0.856829
PRIMER_LEFT_20_PENALTY=0.503705
PRIMER_RIGHT_20_PENALTY=0.353123
PRIMER_LEFT_20_SEQUENCE=TGATCGTCCCATTGTTCTTG
PRIMER_RIGHT_20_SEQUENCE=TGTTCCTATCGCTCCTCCTG
PRIMER_LEFT_20=227,20
PRIMER_RIGHT_20=1096,20
PRIMER_LEFT_20_TM=59.496
PRIMER_RIGHT_20_TM=60.353
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=870
PRIMER_PAIR_21_PENALTY=0.909988
PRIMER_LEFT_21_PENALTY=0.476376
PRIMER_RIGHT_21_PENALTY=0.433612
PRIMER_LEFT_21_SEQUENCE=ATTGTTATACCCGCGCTTCA
PRIMER_RIGHT_21_SEQUENCE=CGCAGCAAAAGATGAGAATG
PRIMER_LEFT_21=423,20
PRIMER_RIGHT_21=1402,20
PRIMER_LEFT_21_TM=60.476
PRIMER_RIGHT_21_TM=59.566
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=980
PRIMER_PAIR_22_PENALTY=0.933652
PRIMER_LEFT_22_PENALTY=0.707509
PRIMER_RIGHT_22_PENALTY=0.226143
PRIMER_LEFT_22_SEQUENCE=AATCATTGGTTCGTTTCCGT
PRIMER_RIGHT_22_SEQUENCE=AGCCATCATTGACAGAAGCC
PRIMER_LEFT_22=873,20
PRIMER_RIGHT_22=1525,20
PRIMER_LEFT_22_TM=59.292
PRIMER_RIGHT_22_TM=60.226
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=653
PRIMER_PAIR_23_PENALTY=0.949380
PRIMER_LEFT_23_PENALTY=0.909234
PRIMER_RIGHT_23_PENALTY=0.040146
PRIMER_LEFT_23_SEQUENCE=ACCGCGATGGTTTTCTTTAC
PRIMER_RIGHT_23_SEQUENCE=TTCGCGTTTTCTCGATCTTT
PRIMER_LEFT_23=895,20
PRIMER_RIGHT_23=1599,20
PRIMER_LEFT_23_TM=59.091
PRIMER_RIGHT_23_TM=59.960
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.0000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=705
PRIMER_PAIR_24_PENALTY=0.949380
PRIMER_LEFT_24_PENALTY=0.909234
PRIMER_RIGHT_24_PENALTY=0.040146
PRIMER_LEFT_24_SEQUENCE=ACCGCGATGGTTTTCTTTAC
PRIMER_RIGHT_24_SEQUENCE=TTTCGCGTTTTCTCGATCTT
PRIMER_LEFT_24=895,20
PRIMER_RIGHT_24=1600,20
PRIMER_LEFT_24_TM=59.091
PRIMER_RIGHT_24_TM=59.960
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.0000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=706
PRIMER_PAIR_25_PENALTY=0.963760
PRIMER_LEFT_25_PENALTY=0.737617
PRIMER_RIGHT_25_PENALTY=0.226143
PRIMER_LEFT_25_SEQUENCE=TGTTGACCCGAGAAAGAACA
PRIMER_RIGHT_25_SEQUENCE=AGCCATCATTGACAGAAGCC
PRIMER_LEFT_25=653,20
PRIMER_RIGHT_25=1525,20
PRIMER_LEFT_25_TM=59.262
PRIMER_RIGHT_25_TM=60.226
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=873
PRIMER_PAIR_26_PENALTY=0.974643
PRIMER_LEFT_26_PENALTY=0.476376
PRIMER_RIGHT_26_PENALTY=0.498267
PRIMER_LEFT_26_SEQUENCE=ATTGTTATACCCGCGCTTCA
PRIMER_RIGHT_26_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_26=423,20
PRIMER_RIGHT_26=1187,20
PRIMER_LEFT_26_TM=60.476
PRIMER_RIGHT_26_TM=59.502
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=765
PRIMER_PAIR_27_PENALTY=0.981137
PRIMER_LEFT_27_PENALTY=0.754994
PRIMER_RIGHT_27_PENALTY=0.226143
PRIMER_LEFT_27_SEQUENCE=ATCATTGGTTCGTTTCCGTG
PRIMER_RIGHT_27_SEQUENCE=AGCCATCATTGACAGAAGCC
PRIMER_LEFT_27=874,20
PRIMER_RIGHT_27=1525,20
PRIMER_LEFT_27_TM=60.755
PRIMER_RIGHT_27_TM=60.226
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=652
PRIMER_PAIR_28_PENALTY=0.989156
PRIMER_LEFT_28_PENALTY=0.636033
PRIMER_RIGHT_28_PENALTY=0.353123
PRIMER_LEFT_28_SEQUENCE=AGCTGGTTTCGGAATGTTGA
PRIMER_RIGHT_28_SEQUENCE=TGTTCCTATCGCTCCTCCTG
PRIMER_LEFT_28=482,20
PRIMER_RIGHT_28=1096,20
PRIMER_LEFT_28_TM=60.636
PRIMER_RIGHT_28_TM=60.353
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=615
PRIMER_PAIR_29_PENALTY=0.989156
PRIMER_LEFT_29_PENALTY=0.636033
PRIMER_RIGHT_29_PENALTY=0.353123
PRIMER_LEFT_29_SEQUENCE=GAGCTGGTTTCGGAATGTTG
PRIMER_RIGHT_29_SEQUENCE=TGTTCCTATCGCTCCTCCTG
PRIMER_LEFT_29=481,20
PRIMER_RIGHT_29=1096,20
PRIMER_LEFT_29_TM=60.636
PRIMER_RIGHT_29_TM=60.353
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=616
PRIMER_PAIR_30_PENALTY=1.001973
PRIMER_LEFT_30_PENALTY=0.503705
PRIMER_RIGHT_30_PENALTY=0.498267
PRIMER_LEFT_30_SEQUENCE=TGATCGTCCCATTGTTCTTG
PRIMER_RIGHT_30_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_30=227,20
PRIMER_RIGHT_30=1187,20
PRIMER_LEFT_30_TM=59.496
PRIMER_RIGHT_30_TM=59.502
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=961
PRIMER_PAIR_31_PENALTY=1.004221
PRIMER_LEFT_31_PENALTY=0.575355
PRIMER_RIGHT_31_PENALTY=0.428866
PRIMER_LEFT_31_SEQUENCE=CGGTGGTATAGGAGGAGGTG
PRIMER_RIGHT_31_SEQUENCE=CATCAGCCTCACGAACAGAG
PRIMER_LEFT_31=518,20
PRIMER_RIGHT_31=1493,20
PRIMER_LEFT_31_TM=59.425
PRIMER_RIGHT_31_TM=59.571
PRIMER_LEFT_31_GC_PERCENT=60.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=976
PRIMER_PAIR_32_PENALTY=1.008967
PRIMER_LEFT_32_PENALTY=0.575355
PRIMER_RIGHT_32_PENALTY=0.433612
PRIMER_LEFT_32_SEQUENCE=CGGTGGTATAGGAGGAGGTG
PRIMER_RIGHT_32_SEQUENCE=CGCAGCAAAAGATGAGAATG
PRIMER_LEFT_32=518,20
PRIMER_RIGHT_32=1402,20
PRIMER_LEFT_32_TM=59.425
PRIMER_RIGHT_32_TM=59.566
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=2.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=885
PRIMER_PAIR_33_PENALTY=1.016923
PRIMER_LEFT_33_PENALTY=0.822322
PRIMER_RIGHT_33_PENALTY=0.194601
PRIMER_LEFT_33_SEQUENCE=GCCAGCAGCTACTTCATTCA
PRIMER_RIGHT_33_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_33=291,20
PRIMER_RIGHT_33=1239,20
PRIMER_LEFT_33_TM=59.178
PRIMER_RIGHT_33_TM=60.195
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=8.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=949
PRIMER_PAIR_34_PENALTY=1.040947
PRIMER_LEFT_34_PENALTY=1.000801
PRIMER_RIGHT_34_PENALTY=0.040146
PRIMER_LEFT_34_SEQUENCE=TTCGAACTCGGTTTGGAATG
PRIMER_RIGHT_34_SEQUENCE=TTCGCGTTTTCTCGATCTTT
PRIMER_LEFT_34=789,20
PRIMER_RIGHT_34=1599,20
PRIMER_LEFT_34_TM=61.001
PRIMER_RIGHT_34_TM=59.960
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=811
PRIMER_PAIR_35_PENALTY=1.040947
PRIMER_LEFT_35_PENALTY=1.000801
PRIMER_RIGHT_35_PENALTY=0.040146
PRIMER_LEFT_35_SEQUENCE=TTCGAACTCGGTTTGGAATG
PRIMER_RIGHT_35_SEQUENCE=TTTCGCGTTTTCTCGATCTT
PRIMER_LEFT_35=789,20
PRIMER_RIGHT_35=1600,20
PRIMER_LEFT_35_TM=61.001
PRIMER_RIGHT_35_TM=59.960
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=6.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=812
PRIMER_PAIR_36_PENALTY=1.069645
PRIMER_LEFT_36_PENALTY=0.636033
PRIMER_RIGHT_36_PENALTY=0.433612
PRIMER_LEFT_36_SEQUENCE=AGCTGGTTTCGGAATGTTGA
PRIMER_RIGHT_36_SEQUENCE=CGCAGCAAAAGATGAGAATG
PRIMER_LEFT_36=482,20
PRIMER_RIGHT_36=1402,20
PRIMER_LEFT_36_TM=60.636
PRIMER_RIGHT_36_TM=59.566
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=921
PRIMER_PAIR_37_PENALTY=1.069645
PRIMER_LEFT_37_PENALTY=0.636033
PRIMER_RIGHT_37_PENALTY=0.433612
PRIMER_LEFT_37_SEQUENCE=GAGCTGGTTTCGGAATGTTG
PRIMER_RIGHT_37_SEQUENCE=CGCAGCAAAAGATGAGAATG
PRIMER_LEFT_37=481,20
PRIMER_RIGHT_37=1402,20
PRIMER_LEFT_37_TM=60.636
PRIMER_RIGHT_37_TM=59.566
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=922
PRIMER_PAIR_38_PENALTY=1.073622
PRIMER_LEFT_38_PENALTY=0.575355
PRIMER_RIGHT_38_PENALTY=0.498267
PRIMER_LEFT_38_SEQUENCE=CGGTGGTATAGGAGGAGGTG
PRIMER_RIGHT_38_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_38=518,20
PRIMER_RIGHT_38=1187,20
PRIMER_LEFT_38_TM=59.425
PRIMER_RIGHT_38_TM=59.502
PRIMER_LEFT_38_GC_PERCENT=60.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=670
PRIMER_PAIR_39_PENALTY=1.090477
PRIMER_LEFT_39_PENALTY=0.895876
PRIMER_RIGHT_39_PENALTY=0.194601
PRIMER_LEFT_39_SEQUENCE=AATTGTTATACCCGCGCTTC
PRIMER_RIGHT_39_SEQUENCE=TAGCTGCTTGTGCTTTGTGG
PRIMER_LEFT_39=422,20
PRIMER_RIGHT_39=1239,20
PRIMER_LEFT_39_TM=59.104
PRIMER_RIGHT_39_TM=60.195
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=818
PRIMER_PAIR_40_PENALTY=1.090740
PRIMER_LEFT_40_PENALTY=0.737617
PRIMER_RIGHT_40_PENALTY=0.353123
PRIMER_LEFT_40_SEQUENCE=TGTTGACCCGAGAAAGAACA
PRIMER_RIGHT_40_SEQUENCE=TGTTCCTATCGCTCCTCCTG
PRIMER_LEFT_40=653,20
PRIMER_RIGHT_40=1096,20
PRIMER_LEFT_40_TM=59.262
PRIMER_RIGHT_40_TM=60.353
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=444
PRIMER_PAIR_41_PENALTY=1.115690
PRIMER_LEFT_41_PENALTY=0.707509
PRIMER_RIGHT_41_PENALTY=0.408180
PRIMER_LEFT_41_SEQUENCE=AATCATTGGTTCGTTTCCGT
PRIMER_RIGHT_41_SEQUENCE=CGCGTTTTCTCGATCTTTCT
PRIMER_LEFT_41=873,20
PRIMER_RIGHT_41=1597,20
PRIMER_LEFT_41_TM=59.292
PRIMER_RIGHT_41_TM=59.592
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.6000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=725
PRIMER_PAIR_42_PENALTY=1.120592
PRIMER_LEFT_42_PENALTY=0.575355
PRIMER_RIGHT_42_PENALTY=0.545237
PRIMER_LEFT_42_SEQUENCE=CGGTGGTATAGGAGGAGGTG
PRIMER_RIGHT_42_SEQUENCE=AGCCTCACGAACAGAGGAAC
PRIMER_LEFT_42=518,20
PRIMER_RIGHT_42=1489,20
PRIMER_LEFT_42_TM=59.425
PRIMER_RIGHT_42_TM=59.455
PRIMER_LEFT_42_GC_PERCENT=60.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=972
PRIMER_PAIR_43_PENALTY=1.134300
PRIMER_LEFT_43_PENALTY=0.636033
PRIMER_RIGHT_43_PENALTY=0.498267
PRIMER_LEFT_43_SEQUENCE=AGCTGGTTTCGGAATGTTGA
PRIMER_RIGHT_43_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_43=482,20
PRIMER_RIGHT_43=1187,20
PRIMER_LEFT_43_TM=60.636
PRIMER_RIGHT_43_TM=59.502
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=706
PRIMER_PAIR_44_PENALTY=1.134300
PRIMER_LEFT_44_PENALTY=0.636033
PRIMER_RIGHT_44_PENALTY=0.498267
PRIMER_LEFT_44_SEQUENCE=GAGCTGGTTTCGGAATGTTG
PRIMER_RIGHT_44_SEQUENCE=TTCCCATGAATGCACTGAAC
PRIMER_LEFT_44=481,20
PRIMER_RIGHT_44=1187,20
PRIMER_LEFT_44_TM=60.636
PRIMER_RIGHT_44_TM=59.502
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=707
PRIMER_PAIR_45_PENALTY=1.135377
PRIMER_LEFT_45_PENALTY=0.909234
PRIMER_RIGHT_45_PENALTY=0.226143
PRIMER_LEFT_45_SEQUENCE=ACCGCGATGGTTTTCTTTAC
PRIMER_RIGHT_45_SEQUENCE=AGCCATCATTGACAGAAGCC
PRIMER_LEFT_45=895,20
PRIMER_RIGHT_45=1525,20
PRIMER_LEFT_45_TM=59.091
PRIMER_RIGHT_45_TM=60.226
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.0000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=631
PRIMER_PAIR_46_PENALTY=1.136375
PRIMER_LEFT_46_PENALTY=0.707509
PRIMER_RIGHT_46_PENALTY=0.428866
PRIMER_LEFT_46_SEQUENCE=AATCATTGGTTCGTTTCCGT
PRIMER_RIGHT_46_SEQUENCE=CATCAGCCTCACGAACAGAG
PRIMER_LEFT_46=873,20
PRIMER_RIGHT_46=1493,20
PRIMER_LEFT_46_TM=59.292
PRIMER_RIGHT_46_TM=59.571
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=621
PRIMER_PAIR_47_PENALTY=1.141121
PRIMER_LEFT_47_PENALTY=0.707509
PRIMER_RIGHT_47_PENALTY=0.433612
PRIMER_LEFT_47_SEQUENCE=AATCATTGGTTCGTTTCCGT
PRIMER_RIGHT_47_SEQUENCE=CGCAGCAAAAGATGAGAATG
PRIMER_LEFT_47=873,20
PRIMER_RIGHT_47=1402,20
PRIMER_LEFT_47_TM=59.292
PRIMER_RIGHT_47_TM=59.566
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=530
PRIMER_PAIR_48_PENALTY=1.153934
PRIMER_LEFT_48_PENALTY=0.347318
PRIMER_RIGHT_48_PENALTY=0.806616
PRIMER_LEFT_48_SEQUENCE=ATAAGGATGGTGCGAGAGGA
PRIMER_RIGHT_48_SEQUENCE=CTTCGAAAGCACGGTCAAAC
PRIMER_LEFT_48=463,20
PRIMER_RIGHT_48=1379,20
PRIMER_LEFT_48_TM=59.653
PRIMER_RIGHT_48_TM=60.807
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=917
PRIMER_PAIR_49_PENALTY=1.159740
PRIMER_LEFT_49_PENALTY=0.353123
PRIMER_RIGHT_49_PENALTY=0.806616
PRIMER_LEFT_49_SEQUENCE=TAAGGATGGTGCGAGAGGAG
PRIMER_RIGHT_49_SEQUENCE=CTTCGAAAGCACGGTCAAAC
PRIMER_LEFT_49=464,20
PRIMER_RIGHT_49=1379,20
PRIMER_LEFT_49_TM=60.353
PRIMER_RIGHT_49_TM=60.807
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=916
=
SEQUENCE_ID=MAGIC.338_2_6697795_6697978_po-0_edi-0_31532_13846_TRUE
SEQUENCE_TARGET=950,283
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTCNATTTTTTCTTTNNNACTCNAAACGNTTTTTCGATAATAAAATNTNGTAACGCATAACATNGATNAAANNNNNCTTTTTNNANTCTTTAAAGGTGGANNAGAGTNNNNNAACGANGACNNATGATANCTNNNNNNNNNNNNNNNNNNNNATCTTCNTNNCACATAAANTANNCTNGAGAANANATCANANTGATATAANNNAAATTAGAGNCTTAATGATTTTAAGTNATACAAGNNTATANGTAAATTTCATTTTANTTAATATTATAAACCATACAAACCTAGTAAANGTTNTAANAAAANTAACATNTTAATACATAAGAAGAGGACACAAGNGTAAACTNTAAAGAGGGANCGGNTAGCATTTTTTCCTTAGATTNTAAAAACTCAATAATCCAATNAAATCAAATGTGATAAAATAGAACTAAAATTCATCATATAGCTTAAGCCCCCCATATATAAGATCCTCACAATGCCTAACAAAAGGAAGAATCTTCCCCGAGTTAGTTCCCNAGTCAGCTTCACATCCATGAGACGGAGGAGTNAGGTAACATGGCTCCATCGACTTAGATCGAACACATGGCGGATTAGGAGTGGTGAAATTTGTTGGCTGATAAAGTAACCATGGCTTTAATCCTCCAAGACTATATGAAACATATCCAAATGTAGACCTTGCGCTCGTGACAATGTTATCGGTTAAGCTCAAAAGATACATCTCAGCAAGCGCCTTTTGGTCGTGACTTTTCTTGTCTGTTTGCTGAACTCTCTCTCCACTTGGNTGATNAACTTCAACAATTTCTCCTGTTGAACTTGGTCGCTTNGAAAACATNTTNGTTAAGTTGCCAGAGAACTCTGGATAGAGAGATGCGACAAGAACAGATTTGAGTTTCGNGNNTTTTGATATATTGACTTTTGATTCCTCTGGTGTAGCAAATTCAGGTAACAGTTTCTCTCTTTGTGTACATGNTANGACCTGGTCAATGACATGTTTGAAACGTCCATCAGGTTTNCCGAAAACCCTTATTTGAATCCCGAGTCTCTCGTCGGCTTTCGACAAATGAGCGTNGTAGTAGTCAGTGACCATATCCCAAACTTCATTTGTAGGGTGAAAAAGATANCGAGCCAAGTGGTGAAAGACGGTTTCTTTCTGCGGGAACAGNTTAACTAGTTCGGTTTGGAAAGTTGGATTAAACCATAACGATGGAACAAAGTAAACATTGGCTTGAANAATCAACCATGGGACTTTGTCAATCAAACTTTGATCCTTTTGGCANACAAACAGCTTATCACTATCCCTTGAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_4986651_4987430_ct-1_rsch-4_353266_225423_TRUE
SEQUENCE_TARGET=950,879
SEQUENCE_TEMPLATE=CGCTTTCGAGTTCTGAATCTTTCCAATATAACATAGGANAAAAAANACTCTGTCCATNTAGTCGTCATGTACATATANNNNTTTGAANAGACTGCTTTTATCCCTCGGTCACTTTTGTATATGNTAGTTTACTCAATTAACCCACAAGACAGTCTCTGTAATTCCAGCAAATATTTTCACCACGTAAAGATTACAGTTTCACAATTTACATGTTATTGTTTTCGTTTTTGTCTTGGTTTAAAGTTNGGAAAAGAAACTACATTATGTTATTTATATGTGACTAGTGTGGGTCTCTGACACTTATAGTTGGATCGTAGTCGTAGGTCCATAAAGACTAGCGTTACAAATTATATTAAAGTTGGTNCACGACATTAATTGCAAACCTCCTTTTTCTTTTTGTTTATTCCAATTTGACCATGTTTGACCTTCTGAAACTACTACATTTTCCGGATTTTGGGTNATATATATGCGATAATGTGATAACTGNTAGAAGAAATATCGTGGAACGTNTTCCAAATTTGTACANACTAATACACTATTATAATATGAAATAATTAGTGGACCCGATGCTTATTGAGAAATTGTTAGGTTAGAAAGTGATATTCTTTTGAATTGATTAAAGGTCCCACTGAANAATTTGACGACTTTTCATATAATTGTTAACTATCAAAATTATTCAGTGGTTTATCAAAATCTGTCTCGAGTCTAATAAGAGTGGCTTTGANTTGTCTTCGAGATTCGTTCTTTGATCNAAAGTTAATGTTTAAGTTCAAAGAATTAATAAGGATGACTAGGAAGTATAGAAGAAAAAGTATAATTGGCAAATGTGATAACAAATCTAACATTGTCTCTATTACTATGATACGCGATTTTTTAAAACATTATGAAATAAATTCAGATTTNAAAAATAACTACGGGACTAGAAAACAATCAGCATGANTTCATAAGAATCAGTAGGATNAAGTCCACCAGCGACGATACGGCGAGGACAATATTCTGTNCAAGAGTTAAATATTAGTATTTGGAAGAGTTATATAAACGAAACCCGATTAGAAATTTCCTCTCTTACAAATTCTAGAAGAAGTAGACAAACCAAAAGGAAATTGAAAAAAGAAACGAAATTCGAATAGACAAACTTTTCACGTTCCAAAACAACTTGTATTAACATATATTTGCTCGTACGTTTTAGAACAGTTGACGCAAATAACTACATAAACTGTGTTTAGATTTTCTGAGTCTCCTTCTCATCTTTCTATTTAACATACACAATATTTTTGTATAAAAATTTGTTGCCCCGTTCGCTTGAGTTGAGTGTAAAAAAACCTTTAAACAACAACCACATTAATATCCCTNAAAAACAACGTCAACATTCTTCTGTAAACTAAAAGTTAAAACTCTACCCGTTAATTAGTATTAGCTCATCATTCAAGTTGATATCTTATCATTATTTGTTTCCCTATACTTTTTAGGATTGAAATAATCAAAAGAAAATTCCTATATCCACGTCAAATTACGGGGTCTTACATTAATACATNNNNNNNNNGTATCAAACAAACTTTACTACTNTTTTCTACGTACGTACGTACCTACTTANCTAAAGCCAGAAACACACTAATCACGACCACGTACTTTTATTTGTTTGCTAAAAGAATTCTTAAAAGTTATTAATGTGNGGGAAAGAAAAATTAGGGTCCAGCCTGGAACTTGCTGCGTTAATTATTANTTTCCTTCGGTCGATGACTCTATTGATCTAGTATCGATGACGTTANACAATTGACTNACAATAATTTTACGAACATCGATCATGCCATAAATCCCACCAAGTAAACATATAGAAATGGTTCACAACTTAAAAACAACTATAATAGAAATGGTTTTAAATGTTGATAATGTCACACACGATTCAGTTATAGAAAACAAAACCTAACGATCTTATANTATATATNNNNNNNNNNNNNNNNNNNNATATATATATATATNTGCCGTACCATAACCAGTGTAGATNAATTTTGACTNAATTAAATTAGTCATTATATAAAAGCTATTATCCTTTTAACAAAGCTACCAAACAAAAACAAAACAATCAATATATACTTATCTATCCTTTTATTGTAACAATAATCTCATGACTTGATGAAAACTAAATAATTCAGTTATTTGTTTCATATATTTCTGGAAAAGAAAACATCAATTATTTCGTTCGAATNAATTATAGTTATCTACTTGNTTTTTTTCCTTAACAGATCTATGAATNATTTCGATGNNNNANACAATTCGCTGGCCCTCTTTGTTTTTCTTTTTCTCAACCTGGCCCACTATGTTTGTTCATGCAAGTAGCAAATTAAAATTCATATCTTCTTTATTAAAACAATAGAAAAGACNAAAANAAAANAAAAAAAATATTCTCTTTTGTTTTAATAATGTTGACTTACTTATCTCCTCTCCTTTTCGAAATGTCTTCATGAGTCTCTCTTCTTCTTCCCCATTCGTCACATAATTAAACATCGCTTCTGACTTCACTCTCACCAAATCTCTCNTCTTTCTCACACACAACAATAAAAATCTNGAAACTAAATAAGAGTTCTCCGACTTCAGATCTTCTCAAACCCTAAATGTTAAAATGTCGTTAAATCGACAACTTCTATTCACTTACTACTTCATCGTCTCTCTTANCCTCTTCTCCGATTTCGTATCCTCTGCAACATTACCAAAAGAAGAAGGTACCAACTTATACAAAAGTTTATGTCTTTGTACGTATATGTTTNATTCGGTGTAATGA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_22108176_22108241_po-0_bur-0_58769_32732_TRUE
SEQUENCE_TARGET=950,165
SEQUENCE_TEMPLATE=TCGAAAAACCCGGCGTAAGCAGTTAAACCTATCATACCTGTTATGAAAAAACAGANCTTTTAAGAGAAATAGGTATNGGTCCATAACACTATTACACTTTTTTTAGTTTCTGTGACTAACCGAGGATTCCAGTATAGTAGCTGAGAGGAACATTGATATTGACGTCGATTTTGAATATAGCAGCCGGGTTGATAGTACTAAACTCCTCCCAGTTCACAATTCCCCAAATCATATCGCCTTTCGTAAAAGATGGATCATCAGAATCTATTACTTGAGACACTCCANTTCCAGCTATAGGCTGCACAAAGAATCAACAACAATTTCTTATTAAGNCCTTGTGTATGTATGTATGTGTGTGGAGAGTTGTTACCTTTCCGGGGATGAAGGCATCCAAAAGTGCTAGAGTGGAAGGAGTTGNTTCTCTCATGAGAATCCCCATGTAAGGGTCACAAGACAAGTAAAGATTCCTCACCATAACCGCCGCTGATCCGGGCTTAACCTTGAGTTCCACGGGGTCTGAAGCTGTGATAATCATTAGGTCAGATTCTTTAGGGATACCCTTCACATAATTTCNCAGTATCACTTGCTTGTTTTTCACNGTGGTCTCCATATTCCTCTGTTTTACTCTTAGTAAAAGCCCTTCTTGCTTTGGTCAGATCTATATANCTTCAAAACTGAACAAAGTTTAAATTGGTGCATCTCCCATCATACATAGAATGGGCTCATCATAGTCATTATCACAGTACTATGATTATGAATTGCATGCATAAAGTATGTTTGGTAAGATAAAATTTAAAATNGTGNNTNGTCTAATTCATAATCATAGTACTGTGATAATGACTAGTGACCAACAAAATTACTANAAAATNTAATTCAAACCACGTATTAGTAGNTACTGTACCANCTCCATTATGTGATGCGACAACACTNTACTTTGTCTGANNCTCTAGATCTGAGGTNTCAGAGAANCTCACCAAATTTGTCTAAAANTCTCTTGAGTNTGACCTAATATCCACGCATTTTGTAGGAATTTTGGAAANAAGAAATTATATTCTAATAGTTTTCNCATGATTTATCATTGNTACATNATTTATTATTAGTCTAGTAGAGTTTGACGAGGTATGNTAAGGTTACTGGTTTAACAGCTGAGGGAGAGACTGCCAATTGTTAAAGAAAGATAGCTCCCTGCAATAAAAGAGGCTCCTGCAACAGTAAGAGCAATGCGACCTGTATTGCAATTCATGAGTTTCATGTTACACAGAATNATTCTCTAAGCTACATGAAATATTNAACTTGGATCTTGCGCTTCGATGAATCTGAAACTCCAAGATGCGTCTTTAGAAGTCTTTCATGTGGATGACTGACCGTGATGTGCGTCCCAAAACCCTGGAGTACCTACGACGAGTTGCCATACGCAGCATTATATGGCGTCACATGTATCATTTCCNAGCCAAGACCAACTCAAATCGGGCATTACAGATTAACGGAGTTTTCGATAGAGGGNCACCAAGACTCACTTTNGCTTNTGTTTAGACCNTAAACNCTCTAATATTCAACAATGTGCTGCCACGATTCATTGNGGTNCTTAATAAGAAANTCATCTTACACATGTCACTTATTTTTTTCAAAANNAATAAATTANTTGAANTAAATTCGATTGAACATNTGTTACTTTATTTTTTATANACTNNNNNNNNNNNNNNNNNNNNNNANTANCCAATNNAAATGTTCTNAGTATTAGCTAGATGAAATATNTCTCACCNTCTCCGTCNNNTNNNATCCNNNNANTTTNCTTNTATATATTGGATTTGTAACATTTCGATTTACTTAGACAACATTGTTTACACGTAATTCATTTATATAGCTGATAATACTATATTAGGCTTAGGCTATAGGCATTATGATGTACAATTTATCGATTTGATTCNTTTTATNAAACACAATTCCCATTCATTTTAAANNNNNNNNTACGTTNATAATCATTGGTTAGTNCGCATTGACTAATGCATAACAAATAGCATCATTAGAAATTGATTTNGNGAANATACTAGACCATAATTTCACG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.279513
PRIMER_LEFT_0_PENALTY=0.241544
PRIMER_RIGHT_0_PENALTY=0.037969
PRIMER_LEFT_0_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_0_SEQUENCE=ATCACGGTCAGTCATCCACA
PRIMER_LEFT_0=633,20
PRIMER_RIGHT_0=1370,20
PRIMER_LEFT_0_TM=60.242
PRIMER_RIGHT_0_TM=59.962
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=738
PRIMER_PAIR_1_PENALTY=0.279513
PRIMER_LEFT_1_PENALTY=0.241544
PRIMER_RIGHT_1_PENALTY=0.037969
PRIMER_LEFT_1_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_1_SEQUENCE=CATCACGGTCAGTCATCCAC
PRIMER_LEFT_1=633,20
PRIMER_RIGHT_1=1371,20
PRIMER_LEFT_1_TM=60.242
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=739
PRIMER_PAIR_2_PENALTY=0.279513
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.037969
PRIMER_LEFT_2_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_2_SEQUENCE=ACATCACGGTCAGTCATCCA
PRIMER_LEFT_2=633,20
PRIMER_RIGHT_2=1372,20
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=740
PRIMER_PAIR_3_PENALTY=0.279513
PRIMER_LEFT_3_PENALTY=0.241544
PRIMER_RIGHT_3_PENALTY=0.037969
PRIMER_LEFT_3_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_3_SEQUENCE=CACATCACGGTCAGTCATCC
PRIMER_LEFT_3=633,20
PRIMER_RIGHT_3=1373,20
PRIMER_LEFT_3_TM=60.242
PRIMER_RIGHT_3_TM=59.962
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=741
PRIMER_PAIR_4_PENALTY=0.293796
PRIMER_LEFT_4_PENALTY=0.096893
PRIMER_RIGHT_4_PENALTY=0.196903
PRIMER_LEFT_4_SEQUENCE=TCCTCCCAGTTCACAATTCC
PRIMER_RIGHT_4_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_4=203,20
PRIMER_RIGHT_4=1165,20
PRIMER_LEFT_4_TM=59.903
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=963
PRIMER_PAIR_5_PENALTY=0.293796
PRIMER_LEFT_5_PENALTY=0.096893
PRIMER_RIGHT_5_PENALTY=0.196903
PRIMER_LEFT_5_SEQUENCE=TCCTCCCAGTTCACAATTCC
PRIMER_RIGHT_5_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_5=203,20
PRIMER_RIGHT_5=1166,20
PRIMER_LEFT_5_TM=59.903
PRIMER_RIGHT_5_TM=59.803
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=964
PRIMER_PAIR_6_PENALTY=0.349902
PRIMER_LEFT_6_PENALTY=0.152999
PRIMER_RIGHT_6_PENALTY=0.196903
PRIMER_LEFT_6_SEQUENCE=GGCTGCACAAAGAATCAACA
PRIMER_RIGHT_6_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_6=296,20
PRIMER_RIGHT_6=1165,20
PRIMER_LEFT_6_TM=59.847
PRIMER_RIGHT_6_TM=59.803
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=870
PRIMER_PAIR_7_PENALTY=0.349902
PRIMER_LEFT_7_PENALTY=0.152999
PRIMER_RIGHT_7_PENALTY=0.196903
PRIMER_LEFT_7_SEQUENCE=GGCTGCACAAAGAATCAACA
PRIMER_RIGHT_7_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_7=296,20
PRIMER_RIGHT_7=1166,20
PRIMER_LEFT_7_TM=59.847
PRIMER_RIGHT_7_TM=59.803
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=871
PRIMER_PAIR_8_PENALTY=0.398725
PRIMER_LEFT_8_PENALTY=0.201822
PRIMER_RIGHT_8_PENALTY=0.196903
PRIMER_LEFT_8_SEQUENCE=CGCCTTTCGTAAAAGATGGA
PRIMER_RIGHT_8_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_8=234,20
PRIMER_RIGHT_8=1165,20
PRIMER_LEFT_8_TM=60.202
PRIMER_RIGHT_8_TM=59.803
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=932
PRIMER_PAIR_9_PENALTY=0.398725
PRIMER_LEFT_9_PENALTY=0.201822
PRIMER_RIGHT_9_PENALTY=0.196903
PRIMER_LEFT_9_SEQUENCE=TCGCCTTTCGTAAAAGATGG
PRIMER_RIGHT_9_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_9=233,20
PRIMER_RIGHT_9=1165,20
PRIMER_LEFT_9_TM=60.202
PRIMER_RIGHT_9_TM=59.803
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=933
PRIMER_PAIR_10_PENALTY=0.398725
PRIMER_LEFT_10_PENALTY=0.201822
PRIMER_RIGHT_10_PENALTY=0.196903
PRIMER_LEFT_10_SEQUENCE=TCGCCTTTCGTAAAAGATGG
PRIMER_RIGHT_10_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_10=233,20
PRIMER_RIGHT_10=1166,20
PRIMER_LEFT_10_TM=60.202
PRIMER_RIGHT_10_TM=59.803
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=934
PRIMER_PAIR_11_PENALTY=0.432848
PRIMER_LEFT_11_PENALTY=0.394879
PRIMER_RIGHT_11_PENALTY=0.037969
PRIMER_LEFT_11_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_11_SEQUENCE=ATCACGGTCAGTCATCCACA
PRIMER_LEFT_11=429,20
PRIMER_RIGHT_11=1370,20
PRIMER_LEFT_11_TM=59.605
PRIMER_RIGHT_11_TM=59.962
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=942
PRIMER_PAIR_12_PENALTY=0.432848
PRIMER_LEFT_12_PENALTY=0.394879
PRIMER_RIGHT_12_PENALTY=0.037969
PRIMER_LEFT_12_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_12_SEQUENCE=CATCACGGTCAGTCATCCAC
PRIMER_LEFT_12=429,20
PRIMER_RIGHT_12=1371,20
PRIMER_LEFT_12_TM=59.605
PRIMER_RIGHT_12_TM=59.962
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=943
PRIMER_PAIR_13_PENALTY=0.432848
PRIMER_LEFT_13_PENALTY=0.394879
PRIMER_RIGHT_13_PENALTY=0.037969
PRIMER_LEFT_13_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_13_SEQUENCE=ACATCACGGTCAGTCATCCA
PRIMER_LEFT_13=429,20
PRIMER_RIGHT_13=1372,20
PRIMER_LEFT_13_TM=59.605
PRIMER_RIGHT_13_TM=59.962
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=944
PRIMER_PAIR_14_PENALTY=0.432848
PRIMER_LEFT_14_PENALTY=0.394879
PRIMER_RIGHT_14_PENALTY=0.037969
PRIMER_LEFT_14_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_14_SEQUENCE=CACATCACGGTCAGTCATCC
PRIMER_LEFT_14=429,20
PRIMER_RIGHT_14=1373,20
PRIMER_LEFT_14_TM=59.605
PRIMER_RIGHT_14_TM=59.962
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=945
PRIMER_PAIR_15_PENALTY=0.438447
PRIMER_LEFT_15_PENALTY=0.241544
PRIMER_RIGHT_15_PENALTY=0.196903
PRIMER_LEFT_15_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_15_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_15=633,20
PRIMER_RIGHT_15=1165,20
PRIMER_LEFT_15_TM=60.242
PRIMER_RIGHT_15_TM=59.803
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=533
PRIMER_PAIR_16_PENALTY=0.438447
PRIMER_LEFT_16_PENALTY=0.241544
PRIMER_RIGHT_16_PENALTY=0.196903
PRIMER_LEFT_16_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_16_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_16=633,20
PRIMER_RIGHT_16=1166,20
PRIMER_LEFT_16_TM=60.242
PRIMER_RIGHT_16_TM=59.803
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=534
PRIMER_PAIR_17_PENALTY=0.459328
PRIMER_LEFT_17_PENALTY=0.241544
PRIMER_RIGHT_17_PENALTY=0.217785
PRIMER_LEFT_17_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_17_SEQUENCE=AGGTACTCCAGGGTTTTGGG
PRIMER_LEFT_17=633,20
PRIMER_RIGHT_17=1396,20
PRIMER_LEFT_17_TM=60.242
PRIMER_RIGHT_17_TM=60.218
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=10.0000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=764
PRIMER_PAIR_18_PENALTY=0.537514
PRIMER_LEFT_18_PENALTY=0.152999
PRIMER_RIGHT_18_PENALTY=0.384515
PRIMER_LEFT_18_SEQUENCE=GGCTGCACAAAGAATCAACA
PRIMER_RIGHT_18_SEQUENCE=CTGTTGCAGGAGCCTCTTTT
PRIMER_LEFT_18=296,20
PRIMER_RIGHT_18=1211,20
PRIMER_LEFT_18_TM=59.847
PRIMER_RIGHT_18_TM=59.615
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=916
PRIMER_PAIR_19_PENALTY=0.591782
PRIMER_LEFT_19_PENALTY=0.394879
PRIMER_RIGHT_19_PENALTY=0.196903
PRIMER_LEFT_19_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_19_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_19=429,20
PRIMER_RIGHT_19=1165,20
PRIMER_LEFT_19_TM=59.605
PRIMER_RIGHT_19_TM=59.803
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=737
PRIMER_PAIR_20_PENALTY=0.591782
PRIMER_LEFT_20_PENALTY=0.394879
PRIMER_RIGHT_20_PENALTY=0.196903
PRIMER_LEFT_20_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_20_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_20=429,20
PRIMER_RIGHT_20=1166,20
PRIMER_LEFT_20_TM=59.605
PRIMER_RIGHT_20_TM=59.803
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=738
PRIMER_PAIR_21_PENALTY=0.612663
PRIMER_LEFT_21_PENALTY=0.394879
PRIMER_RIGHT_21_PENALTY=0.217785
PRIMER_LEFT_21_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_21_SEQUENCE=AGGTACTCCAGGGTTTTGGG
PRIMER_LEFT_21=429,20
PRIMER_RIGHT_21=1396,20
PRIMER_LEFT_21_TM=59.605
PRIMER_RIGHT_21_TM=60.218
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=10.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=968
PRIMER_PAIR_22_PENALTY=0.626059
PRIMER_LEFT_22_PENALTY=0.241544
PRIMER_RIGHT_22_PENALTY=0.384515
PRIMER_LEFT_22_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_22_SEQUENCE=CTGTTGCAGGAGCCTCTTTT
PRIMER_LEFT_22=633,20
PRIMER_RIGHT_22=1211,20
PRIMER_LEFT_22_TM=60.242
PRIMER_RIGHT_22_TM=59.615
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=579
PRIMER_PAIR_23_PENALTY=0.711299
PRIMER_LEFT_23_PENALTY=0.673329
PRIMER_RIGHT_23_PENALTY=0.037969
PRIMER_LEFT_23_SEQUENCE=GGTGCATCTCCCATCATACA
PRIMER_RIGHT_23_SEQUENCE=ATCACGGTCAGTCATCCACA
PRIMER_LEFT_23=692,20
PRIMER_RIGHT_23=1370,20
PRIMER_LEFT_23_TM=59.327
PRIMER_RIGHT_23_TM=59.962
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=5.6000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=679
PRIMER_PAIR_24_PENALTY=0.711299
PRIMER_LEFT_24_PENALTY=0.673329
PRIMER_RIGHT_24_PENALTY=0.037969
PRIMER_LEFT_24_SEQUENCE=GGTGCATCTCCCATCATACA
PRIMER_RIGHT_24_SEQUENCE=CATCACGGTCAGTCATCCAC
PRIMER_LEFT_24=692,20
PRIMER_RIGHT_24=1371,20
PRIMER_LEFT_24_TM=59.327
PRIMER_RIGHT_24_TM=59.962
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=5.6000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=680
PRIMER_PAIR_25_PENALTY=0.711299
PRIMER_LEFT_25_PENALTY=0.673329
PRIMER_RIGHT_25_PENALTY=0.037969
PRIMER_LEFT_25_SEQUENCE=GGTGCATCTCCCATCATACA
PRIMER_RIGHT_25_SEQUENCE=ACATCACGGTCAGTCATCCA
PRIMER_LEFT_25=692,20
PRIMER_RIGHT_25=1372,20
PRIMER_LEFT_25_TM=59.327
PRIMER_RIGHT_25_TM=59.962
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=5.6000
PRIMER_RIGHT_25_END_STABILITY=8.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=681
PRIMER_PAIR_26_PENALTY=0.711299
PRIMER_LEFT_26_PENALTY=0.673329
PRIMER_RIGHT_26_PENALTY=0.037969
PRIMER_LEFT_26_SEQUENCE=GGTGCATCTCCCATCATACA
PRIMER_RIGHT_26_SEQUENCE=CACATCACGGTCAGTCATCC
PRIMER_LEFT_26=692,20
PRIMER_RIGHT_26=1373,20
PRIMER_LEFT_26_TM=59.327
PRIMER_RIGHT_26_TM=59.962
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=5.6000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=682
PRIMER_PAIR_27_PENALTY=0.711299
PRIMER_LEFT_27_PENALTY=0.673329
PRIMER_RIGHT_27_PENALTY=0.037969
PRIMER_LEFT_27_SEQUENCE=TGGTGCATCTCCCATCATAC
PRIMER_RIGHT_27_SEQUENCE=ATCACGGTCAGTCATCCACA
PRIMER_LEFT_27=691,20
PRIMER_RIGHT_27=1370,20
PRIMER_LEFT_27_TM=59.327
PRIMER_RIGHT_27_TM=59.962
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.6000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=680
PRIMER_PAIR_28_PENALTY=0.711299
PRIMER_LEFT_28_PENALTY=0.673329
PRIMER_RIGHT_28_PENALTY=0.037969
PRIMER_LEFT_28_SEQUENCE=TGGTGCATCTCCCATCATAC
PRIMER_RIGHT_28_SEQUENCE=CATCACGGTCAGTCATCCAC
PRIMER_LEFT_28=691,20
PRIMER_RIGHT_28=1371,20
PRIMER_LEFT_28_TM=59.327
PRIMER_RIGHT_28_TM=59.962
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=5.6000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=681
PRIMER_PAIR_29_PENALTY=0.711299
PRIMER_LEFT_29_PENALTY=0.673329
PRIMER_RIGHT_29_PENALTY=0.037969
PRIMER_LEFT_29_SEQUENCE=TGGTGCATCTCCCATCATAC
PRIMER_RIGHT_29_SEQUENCE=ACATCACGGTCAGTCATCCA
PRIMER_LEFT_29=691,20
PRIMER_RIGHT_29=1372,20
PRIMER_LEFT_29_TM=59.327
PRIMER_RIGHT_29_TM=59.962
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=5.6000
PRIMER_RIGHT_29_END_STABILITY=8.1000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=682
PRIMER_PAIR_30_PENALTY=0.711299
PRIMER_LEFT_30_PENALTY=0.673329
PRIMER_RIGHT_30_PENALTY=0.037969
PRIMER_LEFT_30_SEQUENCE=TGGTGCATCTCCCATCATAC
PRIMER_RIGHT_30_SEQUENCE=CACATCACGGTCAGTCATCC
PRIMER_LEFT_30=691,20
PRIMER_RIGHT_30=1373,20
PRIMER_LEFT_30_TM=59.327
PRIMER_RIGHT_30_TM=59.962
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=5.6000
PRIMER_RIGHT_30_END_STABILITY=8.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=683
PRIMER_PAIR_31_PENALTY=0.727616
PRIMER_LEFT_31_PENALTY=0.241544
PRIMER_RIGHT_31_PENALTY=0.486072
PRIMER_LEFT_31_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_31_SEQUENCE=CCGATTTGAGTTGGTCTTGG
PRIMER_LEFT_31=633,20
PRIMER_RIGHT_31=1469,20
PRIMER_LEFT_31_TM=60.242
PRIMER_RIGHT_31_TM=60.486
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=837
PRIMER_PAIR_32_PENALTY=0.727616
PRIMER_LEFT_32_PENALTY=0.241544
PRIMER_RIGHT_32_PENALTY=0.486072
PRIMER_LEFT_32_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_32_SEQUENCE=CCCGATTTGAGTTGGTCTTG
PRIMER_LEFT_32=633,20
PRIMER_RIGHT_32=1470,20
PRIMER_LEFT_32_TM=60.242
PRIMER_RIGHT_32_TM=60.486
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=838
PRIMER_PAIR_33_PENALTY=0.750292
PRIMER_LEFT_33_PENALTY=0.712323
PRIMER_RIGHT_33_PENALTY=0.037969
PRIMER_LEFT_33_SEQUENCE=AAAAGCCCTTCTTGCTTTGG
PRIMER_RIGHT_33_SEQUENCE=ATCACGGTCAGTCATCCACA
PRIMER_LEFT_33=632,20
PRIMER_RIGHT_33=1370,20
PRIMER_LEFT_33_TM=60.712
PRIMER_RIGHT_33_TM=59.962
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.8000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=739
PRIMER_PAIR_34_PENALTY=0.750292
PRIMER_LEFT_34_PENALTY=0.712323
PRIMER_RIGHT_34_PENALTY=0.037969
PRIMER_LEFT_34_SEQUENCE=AAAAGCCCTTCTTGCTTTGG
PRIMER_RIGHT_34_SEQUENCE=CATCACGGTCAGTCATCCAC
PRIMER_LEFT_34=632,20
PRIMER_RIGHT_34=1371,20
PRIMER_LEFT_34_TM=60.712
PRIMER_RIGHT_34_TM=59.962
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=740
PRIMER_PAIR_35_PENALTY=0.750292
PRIMER_LEFT_35_PENALTY=0.712323
PRIMER_RIGHT_35_PENALTY=0.037969
PRIMER_LEFT_35_SEQUENCE=AAAAGCCCTTCTTGCTTTGG
PRIMER_RIGHT_35_SEQUENCE=ACATCACGGTCAGTCATCCA
PRIMER_LEFT_35=632,20
PRIMER_RIGHT_35=1372,20
PRIMER_LEFT_35_TM=60.712
PRIMER_RIGHT_35_TM=59.962
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.8000
PRIMER_RIGHT_35_END_STABILITY=8.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=741
PRIMER_PAIR_36_PENALTY=0.750292
PRIMER_LEFT_36_PENALTY=0.712323
PRIMER_RIGHT_36_PENALTY=0.037969
PRIMER_LEFT_36_SEQUENCE=AAAAGCCCTTCTTGCTTTGG
PRIMER_RIGHT_36_SEQUENCE=CACATCACGGTCAGTCATCC
PRIMER_LEFT_36=632,20
PRIMER_RIGHT_36=1373,20
PRIMER_LEFT_36_TM=60.712
PRIMER_RIGHT_36_TM=59.962
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.8000
PRIMER_RIGHT_36_END_STABILITY=8.1000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=742
PRIMER_PAIR_37_PENALTY=0.779393
PRIMER_LEFT_37_PENALTY=0.394879
PRIMER_RIGHT_37_PENALTY=0.384515
PRIMER_LEFT_37_SEQUENCE=GAATCCCCATGTAAGGGTCA
PRIMER_RIGHT_37_SEQUENCE=CTGTTGCAGGAGCCTCTTTT
PRIMER_LEFT_37=429,20
PRIMER_RIGHT_37=1211,20
PRIMER_LEFT_37_TM=59.605
PRIMER_RIGHT_37_TM=59.615
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=783
PRIMER_PAIR_38_PENALTY=0.780950
PRIMER_LEFT_38_PENALTY=0.742980
PRIMER_RIGHT_38_PENALTY=0.037969
PRIMER_LEFT_38_SEQUENCE=AAGCCCTTCTTGCTTTGGTC
PRIMER_RIGHT_38_SEQUENCE=ATCACGGTCAGTCATCCACA
PRIMER_LEFT_38=634,20
PRIMER_RIGHT_38=1370,20
PRIMER_LEFT_38_TM=60.743
PRIMER_RIGHT_38_TM=59.962
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=737
PRIMER_PAIR_39_PENALTY=0.780950
PRIMER_LEFT_39_PENALTY=0.742980
PRIMER_RIGHT_39_PENALTY=0.037969
PRIMER_LEFT_39_SEQUENCE=AAGCCCTTCTTGCTTTGGTC
PRIMER_RIGHT_39_SEQUENCE=CATCACGGTCAGTCATCCAC
PRIMER_LEFT_39=634,20
PRIMER_RIGHT_39=1371,20
PRIMER_LEFT_39_TM=60.743
PRIMER_RIGHT_39_TM=59.962
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=738
PRIMER_PAIR_40_PENALTY=0.780950
PRIMER_LEFT_40_PENALTY=0.742980
PRIMER_RIGHT_40_PENALTY=0.037969
PRIMER_LEFT_40_SEQUENCE=AAGCCCTTCTTGCTTTGGTC
PRIMER_RIGHT_40_SEQUENCE=ACATCACGGTCAGTCATCCA
PRIMER_LEFT_40=634,20
PRIMER_RIGHT_40=1372,20
PRIMER_LEFT_40_TM=60.743
PRIMER_RIGHT_40_TM=59.962
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=739
PRIMER_PAIR_41_PENALTY=0.780950
PRIMER_LEFT_41_PENALTY=0.742980
PRIMER_RIGHT_41_PENALTY=0.037969
PRIMER_LEFT_41_SEQUENCE=AAGCCCTTCTTGCTTTGGTC
PRIMER_RIGHT_41_SEQUENCE=CACATCACGGTCAGTCATCC
PRIMER_LEFT_41=634,20
PRIMER_RIGHT_41=1373,20
PRIMER_LEFT_41_TM=60.743
PRIMER_RIGHT_41_TM=59.962
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=740
PRIMER_PAIR_42_PENALTY=0.827680
PRIMER_LEFT_42_PENALTY=0.630777
PRIMER_RIGHT_42_PENALTY=0.196903
PRIMER_LEFT_42_SEQUENCE=CCCAAATCATATCGCCTTTC
PRIMER_RIGHT_42_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_42=222,20
PRIMER_RIGHT_42=1165,20
PRIMER_LEFT_42_TM=59.369
PRIMER_RIGHT_42_TM=59.803
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=944
PRIMER_PAIR_43_PENALTY=0.827680
PRIMER_LEFT_43_PENALTY=0.630777
PRIMER_RIGHT_43_PENALTY=0.196903
PRIMER_LEFT_43_SEQUENCE=CCCAAATCATATCGCCTTTC
PRIMER_RIGHT_43_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_43=222,20
PRIMER_RIGHT_43=1166,20
PRIMER_LEFT_43_TM=59.369
PRIMER_RIGHT_43_TM=59.803
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=945
PRIMER_PAIR_44_PENALTY=0.851575
PRIMER_LEFT_44_PENALTY=0.152999
PRIMER_RIGHT_44_PENALTY=0.698576
PRIMER_LEFT_44_SEQUENCE=GGCTGCACAAAGAATCAACA
PRIMER_RIGHT_44_SEQUENCE=CAATACAGGTCGCATTGCTC
PRIMER_LEFT_44=296,20
PRIMER_RIGHT_44=1234,20
PRIMER_LEFT_44_TM=59.847
PRIMER_RIGHT_44_TM=59.301
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=939
PRIMER_PAIR_45_PENALTY=0.870233
PRIMER_LEFT_45_PENALTY=0.673329
PRIMER_RIGHT_45_PENALTY=0.196903
PRIMER_LEFT_45_SEQUENCE=GGTGCATCTCCCATCATACA
PRIMER_RIGHT_45_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_45=692,20
PRIMER_RIGHT_45=1165,20
PRIMER_LEFT_45_TM=59.327
PRIMER_RIGHT_45_TM=59.803
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=5.6000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=474
PRIMER_PAIR_46_PENALTY=0.870233
PRIMER_LEFT_46_PENALTY=0.673329
PRIMER_RIGHT_46_PENALTY=0.196903
PRIMER_LEFT_46_SEQUENCE=GGTGCATCTCCCATCATACA
PRIMER_RIGHT_46_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_46=692,20
PRIMER_RIGHT_46=1166,20
PRIMER_LEFT_46_TM=59.327
PRIMER_RIGHT_46_TM=59.803
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=5.6000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=475
PRIMER_PAIR_47_PENALTY=0.870233
PRIMER_LEFT_47_PENALTY=0.673329
PRIMER_RIGHT_47_PENALTY=0.196903
PRIMER_LEFT_47_SEQUENCE=TGGTGCATCTCCCATCATAC
PRIMER_RIGHT_47_SEQUENCE=AATTGGCAGTCTCTCCCTCA
PRIMER_LEFT_47=691,20
PRIMER_RIGHT_47=1165,20
PRIMER_LEFT_47_TM=59.327
PRIMER_RIGHT_47_TM=59.803
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=5.6000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=475
PRIMER_PAIR_48_PENALTY=0.870233
PRIMER_LEFT_48_PENALTY=0.673329
PRIMER_RIGHT_48_PENALTY=0.196903
PRIMER_LEFT_48_SEQUENCE=TGGTGCATCTCCCATCATAC
PRIMER_RIGHT_48_SEQUENCE=CAATTGGCAGTCTCTCCCTC
PRIMER_LEFT_48=691,20
PRIMER_RIGHT_48=1166,20
PRIMER_LEFT_48_TM=59.327
PRIMER_RIGHT_48_TM=59.803
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.6000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=476
PRIMER_PAIR_49_PENALTY=0.888179
PRIMER_LEFT_49_PENALTY=0.241544
PRIMER_RIGHT_49_PENALTY=0.646636
PRIMER_LEFT_49_SEQUENCE=AAAGCCCTTCTTGCTTTGGT
PRIMER_RIGHT_49_SEQUENCE=TAATGCTGCGTATGGCAACT
PRIMER_LEFT_49=633,20
PRIMER_RIGHT_49=1422,20
PRIMER_LEFT_49_TM=60.242
PRIMER_RIGHT_49_TM=59.353
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=790
=
SEQUENCE_ID=MAGIC.446_4_1390878_1391271_ct-1_rsch-4_145794_68737_TRUE
SEQUENCE_TARGET=950,493
SEQUENCE_TEMPLATE=AGATCAACTGGAGACAAATTTAGATCTTTGTCCATCAAAAAGTGGTCAACAAAACCAAGGGCATCTGCTTGTGTTGACTCTCCTGGTTCTTGAGAATCCAAAAAATCAAGCCCTGGACCATGGCTACTAGCAGTAATCCGCAATGAACCTTGGAAACCGGTCACTGTATCACAATTTGATTCTTGCTTGCCTTGAAGAAAGAGATGTAGTAAGATTTATCTGAGTTAATACAAAGAAGAAGATAGAGGCAACATAACAACACAAGGATATGTTTACCTGTTTGACATTCGTGATCATTTGATCCATCAGACTCCAAAAGTATATCTCCAGCTTTCGGNCTTAGCTCCGGCAAACACATCGGCTCACCAGCAGCGACACTTCCATCTTCATCATCACTATCATCCACCACCACTTGGTCGAGACCGTAACATTCCTGGTTCAGTGATTCCTCGTCACCACAATTTCCGTTATCTACTAGCTCCGTATCGTCGTAGAGCACTTGAAATGGGGTTTCAAGTAACGAGCTATTAAGTACTAATGCTTCATCAATCGCCTCTGTATCTTCAATTTCCCCACATCCCANAATCCGATTAACCTCAAACCCTAAGAGAATCCANAAATGAAATTGGTACTACGAATCTATCACCTCAAAAATCTACAATTATCAGCAAGAAAAAGAAAATGTAAATGAATCGTAATCTCAAAACTCACTATGATCCGAAGAANCNGAAGGAGACTGCAAATCGGAGGCGAAATCTTCGGTTTCCATAAGATTGACCATCGAAACTCGAATTTCAATGCTGTCCAAGAAGAAATGGAATCGCTAGAAGCTNCCGATTCGTATACGGTTGGAATCAGAGAAGAAACCCTAGAGGTTCCATTGGTGCTTTAGTCGAGGTGATTGGCGGGAGAGAGACGAGTACAGAGCTGTGTCGAATGGTTTTGAAAAGCTCAGTTCCGTGAAACGATATTTTAGTTANTATATATATTTANTTATGTCNAANGATACATCAAATAGGGCATTTGGTCTAGTGGTATGATTCTCGCTTAGGGTGCGAGAGGTCCCGAGTTCAATTCTCGGAATGCCCCTCTANTATCTTTTGTTTTTTGGGACTTGAAAATTCTTACCTCATTTTCTTCTCAGTATTTNCACTATGTTCTTATCCCTTTAATCTTCTTCACCTATGTTGCAGGAAAANAAAAGATNATNTATTATGTCTTCTAGAAGGAAACCAAACTACTTATTCGACACCTCATAAATGCTTTGNTATTTTTCTANTCTTTGTNTGCNATTTGGTGAAGAATCTTTGAGAATTTGGTCAAATGAACATTTAAGAATGTTACAATTTATCGACATGTCAAAGATTGGTAACACTTTGGTTTTCTTTTCCATNCCATGAATGTGAACTTGCTTACTTAAAATATTTAACTGTAATTTTATAACATTACTTTACGATATNAATTAATTTAGATTTACCAGCAACACATGGTATTAGGATTGTGTNCCTTTGTAATTGTTGATATATGAGTCACTTGTTCTAAGAACACATTTCATTACTTTGGNTTTTGAAATTCTAAGTTGAAAATCTTTGTTTTATTTTCTTGGCTAGATTTGATGCGTGTCATAATCTAATCCAATTTGAAAATATGTAATATGTTGTATAAAAAGTTAATATGCCAACAAAAGGATAGATCTTAGTTGGTGGAATCAACANCATTAGATATCAAATTGGTCGTATGCAATGTTTCTGTATAATATATCATTTGTCTACTGAATAATATATATCATAATGTTAATTGTTAGCTTTCTGTCGGGTTATGAAGATGGTATTGGTAGGTACGTTGTATAACATAAGAATTGAAAACGTAAATATTATTCATTAGTAGACAGCTCATAATAAGCACACCCACACATGCATGCCACAAGCAATAGAGGTTTTTTAAAAGGTTTTCTTCTTTTAACAAAAGAAAGGCCTTTCTTTGATTTCAAATTTTCAATGATATTAATATTGCAATAAAATACCATGATATAACATATTTTANATNTATATTAATGATTTATGACCCCAAAAGNAAAAANATATTAATTAAAATATGTAGTTCAACAATAACCTCAGCCTATNTGGCTATTATACAATCTTGTTTTGGGAAGAAATCAACACATGCTTATGAACTTTTATGTTCCTTTTTTTGTCTTCATTAGCAAAAGATAAANGGATCAANCCTTTTTTACAAACACTTGCATGCTAGTATGCTTCAGGTTCAATCACCGCCCCATTAGATTCTCTCTCCCTCTCTAAAGACCACACCTTTATGTTCCCTCTATATCTATATAAGACGCAAACACAGGGACCATTCTCTTCNAAGACGAGAAATTATAAGTTAGGAAACGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.503026
PRIMER_LEFT_0_PENALTY=0.335679
PRIMER_RIGHT_0_PENALTY=0.167348
PRIMER_LEFT_0_SEQUENCE=CGAATTTCAATGCTGTCCAA
PRIMER_RIGHT_0_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_0=788,20
PRIMER_RIGHT_0=1621,20
PRIMER_LEFT_0_TM=59.664
PRIMER_RIGHT_0_TM=59.833
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=834
PRIMER_PAIR_1_PENALTY=0.727332
PRIMER_LEFT_1_PENALTY=0.559985
PRIMER_RIGHT_1_PENALTY=0.167348
PRIMER_LEFT_1_SEQUENCE=TGGTGCTTTAGTCGAGGTGA
PRIMER_RIGHT_1_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_1=881,20
PRIMER_RIGHT_1=1621,20
PRIMER_LEFT_1_TM=59.440
PRIMER_RIGHT_1_TM=59.833
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=741
PRIMER_PAIR_2_PENALTY=0.766833
PRIMER_LEFT_2_PENALTY=0.599485
PRIMER_RIGHT_2_PENALTY=0.167348
PRIMER_LEFT_2_SEQUENCE=CCCTAGAGGTTCCATTGGTG
PRIMER_RIGHT_2_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_2=866,20
PRIMER_RIGHT_2=1621,20
PRIMER_LEFT_2_TM=59.401
PRIMER_RIGHT_2_TM=59.833
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=756
PRIMER_PAIR_3_PENALTY=1.285820
PRIMER_LEFT_3_PENALTY=1.118473
PRIMER_RIGHT_3_PENALTY=0.167348
PRIMER_LEFT_3_SEQUENCE=CAGAGCTGTGTCGAATGGTT
PRIMER_RIGHT_3_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_3=922,20
PRIMER_RIGHT_3=1621,20
PRIMER_LEFT_3_TM=58.882
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=700
PRIMER_PAIR_4_PENALTY=1.319912
PRIMER_LEFT_4_PENALTY=1.152564
PRIMER_RIGHT_4_PENALTY=0.167348
PRIMER_LEFT_4_SEQUENCE=TGCTGTCCAAGAAGAAATGG
PRIMER_RIGHT_4_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_4=798,20
PRIMER_RIGHT_4=1621,20
PRIMER_LEFT_4_TM=58.847
PRIMER_RIGHT_4_TM=59.833
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=824
PRIMER_PAIR_5_PENALTY=1.392263
PRIMER_LEFT_5_PENALTY=1.224915
PRIMER_RIGHT_5_PENALTY=0.167348
PRIMER_LEFT_5_SEQUENCE=AGCTGTGTCGAATGGTTTTG
PRIMER_RIGHT_5_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_5=925,20
PRIMER_RIGHT_5=1621,20
PRIMER_LEFT_5_TM=58.775
PRIMER_RIGHT_5_TM=59.833
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=10.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=697
PRIMER_PAIR_6_PENALTY=1.399593
PRIMER_LEFT_6_PENALTY=1.232246
PRIMER_RIGHT_6_PENALTY=0.167348
PRIMER_LEFT_6_SEQUENCE=ACCCTAGAGGTTCCATTGGTG
PRIMER_RIGHT_6_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_6=865,21
PRIMER_RIGHT_6=1621,20
PRIMER_LEFT_6_TM=60.232
PRIMER_RIGHT_6_TM=59.833
PRIMER_LEFT_6_GC_PERCENT=52.381
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=10.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=757
PRIMER_PAIR_7_PENALTY=1.473067
PRIMER_LEFT_7_PENALTY=1.305719
PRIMER_RIGHT_7_PENALTY=0.167348
PRIMER_LEFT_7_SEQUENCE=CAGAGCTGTGTCGAATGGTTT
PRIMER_RIGHT_7_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_7=922,21
PRIMER_RIGHT_7=1621,20
PRIMER_LEFT_7_TM=60.306
PRIMER_RIGHT_7_TM=59.833
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=10.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=700
PRIMER_PAIR_8_PENALTY=1.560118
PRIMER_LEFT_8_PENALTY=1.392770
PRIMER_RIGHT_8_PENALTY=0.167348
PRIMER_LEFT_8_SEQUENCE=CGGGAGAGAGACGAGTACAGA
PRIMER_RIGHT_8_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_8=905,21
PRIMER_RIGHT_8=1621,20
PRIMER_LEFT_8_TM=59.607
PRIMER_RIGHT_8_TM=59.833
PRIMER_LEFT_8_GC_PERCENT=57.143
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=717
PRIMER_PAIR_9_PENALTY=1.659015
PRIMER_LEFT_9_PENALTY=0.599485
PRIMER_RIGHT_9_PENALTY=1.059530
PRIMER_LEFT_9_SEQUENCE=CCCTAGAGGTTCCATTGGTG
PRIMER_RIGHT_9_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_9=866,20
PRIMER_RIGHT_9=1827,21
PRIMER_LEFT_9_TM=59.401
PRIMER_RIGHT_9_TM=59.940
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=962
PRIMER_PAIR_10_PENALTY=1.669455
PRIMER_LEFT_10_PENALTY=1.502108
PRIMER_RIGHT_10_PENALTY=0.167348
PRIMER_LEFT_10_SEQUENCE=CCTAGAGGTTCCATTGGTGCT
PRIMER_RIGHT_10_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_10=867,21
PRIMER_RIGHT_10=1621,20
PRIMER_LEFT_10_TM=60.502
PRIMER_RIGHT_10_TM=59.833
PRIMER_LEFT_10_GC_PERCENT=52.381
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=10.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=755
PRIMER_PAIR_11_PENALTY=1.736149
PRIMER_LEFT_11_PENALTY=0.335679
PRIMER_RIGHT_11_PENALTY=1.400471
PRIMER_LEFT_11_SEQUENCE=CGAATTTCAATGCTGTCCAA
PRIMER_RIGHT_11_SEQUENCE=CAGAAACATTGCATACGACCA
PRIMER_LEFT_11=788,20
PRIMER_RIGHT_11=1750,21
PRIMER_LEFT_11_TM=59.664
PRIMER_RIGHT_11_TM=59.600
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=963
PRIMER_PAIR_12_PENALTY=1.790010
PRIMER_LEFT_12_PENALTY=1.622662
PRIMER_RIGHT_12_PENALTY=0.167348
PRIMER_LEFT_12_SEQUENCE=CGAATTTCAATGCTGTCCAAG
PRIMER_RIGHT_12_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_12=788,21
PRIMER_RIGHT_12=1621,20
PRIMER_LEFT_12_TM=60.623
PRIMER_RIGHT_12_TM=59.833
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=10.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=834
PRIMER_PAIR_13_PENALTY=1.850942
PRIMER_LEFT_13_PENALTY=1.683594
PRIMER_RIGHT_13_PENALTY=0.167348
PRIMER_LEFT_13_SEQUENCE=GAGCTGTGTCGAATGGTTTTG
PRIMER_RIGHT_13_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_13=924,21
PRIMER_RIGHT_13=1621,20
PRIMER_LEFT_13_TM=60.684
PRIMER_RIGHT_13_TM=59.833
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=10.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=698
PRIMER_PAIR_14_PENALTY=1.855751
PRIMER_LEFT_14_PENALTY=1.688403
PRIMER_RIGHT_14_PENALTY=0.167348
PRIMER_LEFT_14_SEQUENCE=AGGAGACTGCAAATCGGAGG
PRIMER_RIGHT_14_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_14=730,20
PRIMER_RIGHT_14=1621,20
PRIMER_LEFT_14_TM=61.688
PRIMER_RIGHT_14_TM=59.833
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=10.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=892
PRIMER_PAIR_15_PENALTY=1.865053
PRIMER_LEFT_15_PENALTY=1.697705
PRIMER_RIGHT_15_PENALTY=0.167348
PRIMER_LEFT_15_SEQUENCE=GCTGTCCAAGAAGAAATGGAA
PRIMER_RIGHT_15_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_15=799,21
PRIMER_RIGHT_15=1621,20
PRIMER_LEFT_15_TM=59.302
PRIMER_RIGHT_15_TM=59.833
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=10.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=823
PRIMER_PAIR_16_PENALTY=1.869229
PRIMER_LEFT_16_PENALTY=1.701882
PRIMER_RIGHT_16_PENALTY=0.167348
PRIMER_LEFT_16_SEQUENCE=CTCGAATTTCAATGCTGTCC
PRIMER_RIGHT_16_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_16=786,20
PRIMER_RIGHT_16=1621,20
PRIMER_LEFT_16_TM=58.298
PRIMER_RIGHT_16_TM=59.833
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=10.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=836
PRIMER_PAIR_17_PENALTY=1.869288
PRIMER_LEFT_17_PENALTY=1.701940
PRIMER_RIGHT_17_PENALTY=0.167348
PRIMER_LEFT_17_SEQUENCE=CGGTTGGAATCAGAGAAGAAA
PRIMER_RIGHT_17_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_17=845,21
PRIMER_RIGHT_17=1621,20
PRIMER_LEFT_17_TM=59.298
PRIMER_RIGHT_17_TM=59.833
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=10.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=777
PRIMER_PAIR_18_PENALTY=1.927659
PRIMER_LEFT_18_PENALTY=1.760311
PRIMER_RIGHT_18_PENALTY=0.167348
PRIMER_LEFT_18_SEQUENCE=TGCTGTCCAAGAAGAAATGGA
PRIMER_RIGHT_18_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_18=798,21
PRIMER_RIGHT_18=1621,20
PRIMER_LEFT_18_TM=60.760
PRIMER_RIGHT_18_TM=59.833
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=10.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=824
PRIMER_PAIR_19_PENALTY=1.960455
PRIMER_LEFT_19_PENALTY=0.559985
PRIMER_RIGHT_19_PENALTY=1.400471
PRIMER_LEFT_19_SEQUENCE=TGGTGCTTTAGTCGAGGTGA
PRIMER_RIGHT_19_SEQUENCE=CAGAAACATTGCATACGACCA
PRIMER_LEFT_19=881,20
PRIMER_RIGHT_19=1750,21
PRIMER_LEFT_19_TM=59.440
PRIMER_RIGHT_19_TM=59.600
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=870
PRIMER_PAIR_20_PENALTY=1.979054
PRIMER_LEFT_20_PENALTY=1.811706
PRIMER_RIGHT_20_PENALTY=0.167348
PRIMER_LEFT_20_SEQUENCE=TTGGTGCTTTAGTCGAGGTGA
PRIMER_RIGHT_20_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_20=880,21
PRIMER_RIGHT_20=1621,20
PRIMER_LEFT_20_TM=60.812
PRIMER_RIGHT_20_TM=59.833
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=10.0000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=742
PRIMER_PAIR_21_PENALTY=1.979133
PRIMER_LEFT_21_PENALTY=1.811785
PRIMER_RIGHT_21_PENALTY=0.167348
PRIMER_LEFT_21_SEQUENCE=ACTCGAATTTCAATGCTGTCC
PRIMER_RIGHT_21_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_21=785,21
PRIMER_RIGHT_21=1621,20
PRIMER_LEFT_21_TM=59.188
PRIMER_RIGHT_21_TM=59.833
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=10.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=837
PRIMER_PAIR_22_PENALTY=1.982952
PRIMER_LEFT_22_PENALTY=1.815604
PRIMER_RIGHT_22_PENALTY=0.167348
PRIMER_LEFT_22_SEQUENCE=ATGCTGTCCAAGAAGAAATGG
PRIMER_RIGHT_22_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_22=797,21
PRIMER_RIGHT_22=1621,20
PRIMER_LEFT_22_TM=59.184
PRIMER_RIGHT_22_TM=59.833
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.4000
PRIMER_RIGHT_22_END_STABILITY=10.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=825
PRIMER_PAIR_23_PENALTY=1.987968
PRIMER_LEFT_23_PENALTY=0.335679
PRIMER_RIGHT_23_PENALTY=1.652290
PRIMER_LEFT_23_SEQUENCE=CGAATTTCAATGCTGTCCAA
PRIMER_RIGHT_23_SEQUENCE=ACACGCATCAAATCTAGCCAA
PRIMER_LEFT_23=788,20
PRIMER_RIGHT_23=1622,21
PRIMER_LEFT_23_TM=59.664
PRIMER_RIGHT_23_TM=60.652
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=10.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=835
PRIMER_PAIR_24_PENALTY=1.999956
PRIMER_LEFT_24_PENALTY=0.599485
PRIMER_RIGHT_24_PENALTY=1.400471
PRIMER_LEFT_24_SEQUENCE=CCCTAGAGGTTCCATTGGTG
PRIMER_RIGHT_24_SEQUENCE=CAGAAACATTGCATACGACCA
PRIMER_LEFT_24=866,20
PRIMER_RIGHT_24=1750,21
PRIMER_LEFT_24_TM=59.401
PRIMER_RIGHT_24_TM=59.600
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=885
PRIMER_PAIR_25_PENALTY=2.009591
PRIMER_LEFT_25_PENALTY=0.335679
PRIMER_RIGHT_25_PENALTY=1.673912
PRIMER_LEFT_25_SEQUENCE=CGAATTTCAATGCTGTCCAA
PRIMER_RIGHT_25_SEQUENCE=GACACGCATCAAATCTAGCC
PRIMER_LEFT_25=788,20
PRIMER_RIGHT_25=1623,20
PRIMER_LEFT_25_TM=59.664
PRIMER_RIGHT_25_TM=58.326
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.7000
PRIMER_PAIR_25_COMPL_ANY=7.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=836
PRIMER_PAIR_26_PENALTY=2.136479
PRIMER_LEFT_26_PENALTY=1.076949
PRIMER_RIGHT_26_PENALTY=1.059530
PRIMER_LEFT_26_SEQUENCE=TTGGTGCTTTAGTCGAGGTG
PRIMER_RIGHT_26_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_26=880,20
PRIMER_RIGHT_26=1827,21
PRIMER_LEFT_26_TM=58.923
PRIMER_RIGHT_26_TM=59.940
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=948
PRIMER_PAIR_27_PENALTY=2.178002
PRIMER_LEFT_27_PENALTY=1.118473
PRIMER_RIGHT_27_PENALTY=1.059530
PRIMER_LEFT_27_SEQUENCE=CAGAGCTGTGTCGAATGGTT
PRIMER_RIGHT_27_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_27=922,20
PRIMER_RIGHT_27=1827,21
PRIMER_LEFT_27_TM=58.882
PRIMER_RIGHT_27_TM=59.940
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=906
PRIMER_PAIR_28_PENALTY=2.212275
PRIMER_LEFT_28_PENALTY=0.559985
PRIMER_RIGHT_28_PENALTY=1.652290
PRIMER_LEFT_28_SEQUENCE=TGGTGCTTTAGTCGAGGTGA
PRIMER_RIGHT_28_SEQUENCE=ACACGCATCAAATCTAGCCAA
PRIMER_LEFT_28=881,20
PRIMER_RIGHT_28=1622,21
PRIMER_LEFT_28_TM=59.440
PRIMER_RIGHT_28_TM=60.652
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=742
PRIMER_PAIR_29_PENALTY=2.233897
PRIMER_LEFT_29_PENALTY=0.559985
PRIMER_RIGHT_29_PENALTY=1.673912
PRIMER_LEFT_29_SEQUENCE=TGGTGCTTTAGTCGAGGTGA
PRIMER_RIGHT_29_SEQUENCE=GACACGCATCAAATCTAGCC
PRIMER_LEFT_29=881,20
PRIMER_RIGHT_29=1623,20
PRIMER_LEFT_29_TM=59.440
PRIMER_RIGHT_29_TM=58.326
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=743
PRIMER_PAIR_30_PENALTY=2.251775
PRIMER_LEFT_30_PENALTY=0.599485
PRIMER_RIGHT_30_PENALTY=1.652290
PRIMER_LEFT_30_SEQUENCE=CCCTAGAGGTTCCATTGGTG
PRIMER_RIGHT_30_SEQUENCE=ACACGCATCAAATCTAGCCAA
PRIMER_LEFT_30=866,20
PRIMER_RIGHT_30=1622,21
PRIMER_LEFT_30_TM=59.401
PRIMER_RIGHT_30_TM=60.652
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=10.0000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=757
PRIMER_PAIR_31_PENALTY=2.273397
PRIMER_LEFT_31_PENALTY=0.599485
PRIMER_RIGHT_31_PENALTY=1.673912
PRIMER_LEFT_31_SEQUENCE=CCCTAGAGGTTCCATTGGTG
PRIMER_RIGHT_31_SEQUENCE=GACACGCATCAAATCTAGCC
PRIMER_LEFT_31=866,20
PRIMER_RIGHT_31=1623,20
PRIMER_LEFT_31_TM=59.401
PRIMER_RIGHT_31_TM=58.326
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=758
PRIMER_PAIR_32_PENALTY=2.274770
PRIMER_LEFT_32_PENALTY=2.107422
PRIMER_RIGHT_32_PENALTY=0.167348
PRIMER_LEFT_32_SEQUENCE=TCGGTTTCCATAAGATTGACC
PRIMER_RIGHT_32_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_32=758,21
PRIMER_RIGHT_32=1621,20
PRIMER_LEFT_32_TM=58.893
PRIMER_RIGHT_32_TM=59.833
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=10.0000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=864
PRIMER_PAIR_33_PENALTY=2.276858
PRIMER_LEFT_33_PENALTY=2.109510
PRIMER_RIGHT_33_PENALTY=0.167348
PRIMER_LEFT_33_SEQUENCE=CGGTTGGAATCAGAGAAGAAAC
PRIMER_RIGHT_33_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_33=845,22
PRIMER_RIGHT_33=1621,20
PRIMER_LEFT_33_TM=60.110
PRIMER_RIGHT_33_TM=59.833
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=777
PRIMER_PAIR_34_PENALTY=2.276858
PRIMER_LEFT_34_PENALTY=2.109510
PRIMER_RIGHT_34_PENALTY=0.167348
PRIMER_LEFT_34_SEQUENCE=ACGGTTGGAATCAGAGAAGAAA
PRIMER_RIGHT_34_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_34=844,22
PRIMER_RIGHT_34=1621,20
PRIMER_LEFT_34_TM=60.110
PRIMER_RIGHT_34_TM=59.833
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=10.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=778
PRIMER_PAIR_35_PENALTY=2.284445
PRIMER_LEFT_35_PENALTY=1.224915
PRIMER_RIGHT_35_PENALTY=1.059530
PRIMER_LEFT_35_SEQUENCE=AGCTGTGTCGAATGGTTTTG
PRIMER_RIGHT_35_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_35=925,20
PRIMER_RIGHT_35=1827,21
PRIMER_LEFT_35_TM=58.775
PRIMER_RIGHT_35_TM=59.940
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.6000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=903
PRIMER_PAIR_36_PENALTY=2.291775
PRIMER_LEFT_36_PENALTY=1.232246
PRIMER_RIGHT_36_PENALTY=1.059530
PRIMER_LEFT_36_SEQUENCE=ACCCTAGAGGTTCCATTGGTG
PRIMER_RIGHT_36_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_36=865,21
PRIMER_RIGHT_36=1827,21
PRIMER_LEFT_36_TM=60.232
PRIMER_RIGHT_36_TM=59.940
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=963
PRIMER_PAIR_37_PENALTY=2.301875
PRIMER_LEFT_37_PENALTY=2.134527
PRIMER_RIGHT_37_PENALTY=0.167348
PRIMER_LEFT_37_SEQUENCE=CGGTTGGAATCAGAGAAGAA
PRIMER_RIGHT_37_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_37=845,20
PRIMER_RIGHT_37=1621,20
PRIMER_LEFT_37_TM=57.865
PRIMER_RIGHT_37_TM=59.833
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=10.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=777
PRIMER_PAIR_38_PENALTY=2.302548
PRIMER_LEFT_38_PENALTY=0.185267
PRIMER_RIGHT_38_PENALTY=2.117281
PRIMER_LEFT_38_SEQUENCE=AATGCTTCATCAATCGCCTC
PRIMER_RIGHT_38_SEQUENCE=TCCTAATACCATGTGTTGCTGG
PRIMER_LEFT_38=536,20
PRIMER_RIGHT_38=1497,22
PRIMER_LEFT_38_TM=60.185
PRIMER_RIGHT_38_TM=59.883
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=962
PRIMER_PAIR_39_PENALTY=2.303207
PRIMER_LEFT_39_PENALTY=2.135860
PRIMER_RIGHT_39_PENALTY=0.167348
PRIMER_LEFT_39_SEQUENCE=GCTGTCCAAGAAGAAATGGA
PRIMER_RIGHT_39_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_39=799,20
PRIMER_RIGHT_39=1621,20
PRIMER_LEFT_39_TM=57.864
PRIMER_RIGHT_39_TM=59.833
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.1000
PRIMER_RIGHT_39_END_STABILITY=10.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=823
PRIMER_PAIR_40_PENALTY=2.308947
PRIMER_LEFT_40_PENALTY=2.141600
PRIMER_RIGHT_40_PENALTY=0.167348
PRIMER_LEFT_40_SEQUENCE=GTGATTGGCGGGAGAGAGAC
PRIMER_RIGHT_40_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_40=897,20
PRIMER_RIGHT_40=1621,20
PRIMER_LEFT_40_TM=62.142
PRIMER_RIGHT_40_TM=59.833
PRIMER_LEFT_40_GC_PERCENT=60.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.1000
PRIMER_RIGHT_40_END_STABILITY=10.0000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=725
PRIMER_PAIR_41_PENALTY=2.313829
PRIMER_LEFT_41_PENALTY=2.146481
PRIMER_RIGHT_41_PENALTY=0.167348
PRIMER_LEFT_41_SEQUENCE=AGAGCTGTGTCGAATGGTTTT
PRIMER_RIGHT_41_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_41=923,21
PRIMER_RIGHT_41=1621,20
PRIMER_LEFT_41_TM=58.854
PRIMER_RIGHT_41_TM=59.833
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=10.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=699
PRIMER_PAIR_42_PENALTY=2.314305
PRIMER_LEFT_42_PENALTY=0.197024
PRIMER_RIGHT_42_PENALTY=2.117281
PRIMER_LEFT_42_SEQUENCE=ATGCTTCATCAATCGCCTCT
PRIMER_RIGHT_42_SEQUENCE=TCCTAATACCATGTGTTGCTGG
PRIMER_LEFT_42=537,20
PRIMER_RIGHT_42=1497,22
PRIMER_LEFT_42_TM=59.803
PRIMER_RIGHT_42_TM=59.883
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=9.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=961
PRIMER_PAIR_43_PENALTY=2.315205
PRIMER_LEFT_43_PENALTY=1.255675
PRIMER_RIGHT_43_PENALTY=1.059530
PRIMER_LEFT_43_SEQUENCE=TGCTTTAGTCGAGGTGATTGG
PRIMER_RIGHT_43_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_43=884,21
PRIMER_RIGHT_43=1827,21
PRIMER_LEFT_43_TM=60.256
PRIMER_RIGHT_43_TM=59.940
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=944
PRIMER_PAIR_44_PENALTY=2.351182
PRIMER_LEFT_44_PENALTY=2.183834
PRIMER_RIGHT_44_PENALTY=0.167348
PRIMER_LEFT_44_SEQUENCE=TTCGGTTTCCATAAGATTGACC
PRIMER_RIGHT_44_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_44=757,22
PRIMER_RIGHT_44=1621,20
PRIMER_LEFT_44_TM=60.184
PRIMER_RIGHT_44_TM=59.833
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=10.0000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=865
PRIMER_PAIR_45_PENALTY=2.365249
PRIMER_LEFT_45_PENALTY=1.305719
PRIMER_RIGHT_45_PENALTY=1.059530
PRIMER_LEFT_45_SEQUENCE=CAGAGCTGTGTCGAATGGTTT
PRIMER_RIGHT_45_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_45=922,21
PRIMER_RIGHT_45=1827,21
PRIMER_LEFT_45_TM=60.306
PRIMER_RIGHT_45_TM=59.940
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=906
PRIMER_PAIR_46_PENALTY=2.371949
PRIMER_LEFT_46_PENALTY=2.204601
PRIMER_RIGHT_46_PENALTY=0.167348
PRIMER_LEFT_46_SEQUENCE=GAGCTGTGTCGAATGGTTTT
PRIMER_RIGHT_46_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_46=924,20
PRIMER_RIGHT_46=1621,20
PRIMER_LEFT_46_TM=57.795
PRIMER_RIGHT_46_TM=59.833
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=10.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=698
PRIMER_PAIR_47_PENALTY=2.389414
PRIMER_LEFT_47_PENALTY=2.222066
PRIMER_RIGHT_47_PENALTY=0.167348
PRIMER_LEFT_47_SEQUENCE=ACGGTTGGAATCAGAGAAGAA
PRIMER_RIGHT_47_SEQUENCE=CACGCATCAAATCTAGCCAA
PRIMER_LEFT_47=844,21
PRIMER_RIGHT_47=1621,20
PRIMER_LEFT_47_TM=58.778
PRIMER_RIGHT_47_TM=59.833
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=10.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=778
PRIMER_PAIR_48_PENALTY=2.390761
PRIMER_LEFT_48_PENALTY=0.273480
PRIMER_RIGHT_48_PENALTY=2.117281
PRIMER_LEFT_48_SEQUENCE=TCTTCAATTTCCCCACATCC
PRIMER_RIGHT_48_SEQUENCE=TCCTAATACCATGTGTTGCTGG
PRIMER_LEFT_48=560,20
PRIMER_RIGHT_48=1497,22
PRIMER_LEFT_48_TM=59.727
PRIMER_RIGHT_48_TM=59.883
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.1000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=938
PRIMER_PAIR_49_PENALTY=2.413682
PRIMER_LEFT_49_PENALTY=1.354152
PRIMER_RIGHT_49_PENALTY=1.059530
PRIMER_LEFT_49_SEQUENCE=AGAGGTTCCATTGGTGCTTT
PRIMER_RIGHT_49_SEQUENCE=CCATCTTCATAACCCGACAGA
PRIMER_LEFT_49=870,20
PRIMER_RIGHT_49=1827,21
PRIMER_LEFT_49_TM=58.646
PRIMER_RIGHT_49_TM=59.940
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=958
=
SEQUENCE_ID=MAGIC.329_3_9016955_9017161_wil-2_zu-0_1258_1320552_TRUE
SEQUENCE_TARGET=950,306
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATANTGAATATAGNTACNAAATAATATAATGATANATTATAATATGACAAATCACAATTTATATNTTTATGTTGTTCGATTTGTGACTACNTATTTGTGTTTGAAAAGTAGTATGATATGTTTTATTNACTACAAGAAAGACAACATTNAATTCNTTTTTGTTTGAATAACGTAACTNAATCGACCACTAAACTATCATACACGTNCTTAAAAAATGATGTTAAGTTTAATGTGATCCCCAAGTGTTGNAATTGGGTTGAGTAATAAATAGAAGTGGACAAATGGTTANTNANTTATTTTCCTAGCTAAATTCAAGTATTTTTCAAAATTATTTTGTTTCAAANTAAAACTTNAAAGTCATAACAAATTCTANNGAGTTTTAAAAATGACCAACAAATTGAAAGTGTGGTTGTTGCAAATGNAACTTNGTGGAGATACGGTCAGTGACAGCCNNACACGACTCCATCGGTTCGATCAAATACCTCACGTAACCCGCCGATTCACTCGATCGTCTTTATTATTCTTTTCNATTTTCAATCATATTGAGCCTGATTGATAATTACAAGTCTCATTACAAACGTATTAGCAACGGTATACAGTCAATATCCGTCACTAAAAATGCTAATTGCAACGAAAAGTATTCTTCTTTCNCTATGTAACTATAATGTGATATAGAATTAGCCCAAAANAAATTGTNTATGATTAATTAGATCTAAAATAAACNATGGTCGTCATGTGACGTTGATCAANCGATAAAGAGTAGTCTGAAATTTACTAATNAAAGTCTTAATTTGTACTTTTTACGAATTATAGTTTAAATGGACACAAAAGTAAATAGATTAANAAAACTAGAAANACGTGGAAATTTACACAAGCACTATGCATGACTGCCTGCATTCCCTCCAAGAGTTTTCTTACCCTAATNAGTACCGGTTCANGAAAATGAAGGTTCTACAAATTATCAATTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_3977853_3978206_edi-0_sf-2_476741_7562_TRUE
SEQUENCE_TARGET=950,453
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_10481106_10481119_oy-0_kn-0_2601_5862_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_126465_126646_zu-0_ct-1_2866_673170_TRUE
SEQUENCE_TARGET=950,281
SEQUENCE_TEMPLATE=CTTNTATATTACCTGGAGAGACTTGAAGNTANCTAGATTCTTAGCCAAAATTCTCGCTGNGAGATAAAGAAAGAATTCTANATTTTGCTTGGCAAAGAGGGNAAGAAGATGANGAAGAAGAAGANACAGNTTTATTTCGTCAACTCTGCTTCTTATTCGGTAACANCGACGTCGTTTTAGTCACCTTCAACTTCTGGGCCTTTTGGGCCTANTGATTCAAATTTGTTGGGCTTNATGCAAGTTTGATTAATTTGGTGCAAAACCTTATTAANAAACACACNCACACAAANNNNNNNNNTTGTGAATTTACTTAACTACGTACATATATATNATCATTAATTAATCACGGTGNTTNTCAAATATATATGGTTACAAATTCAATATGTTTNGGACAAAATAATNTGTGCCTAAATCTGCTTATAAATNACAAAATATATACAACCACTAAAAATAGAAGAGATATTTATCATGAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATTTTGCAAATACTTGGCTACAATNNAAAAAACTGATAGATTTGTGTGNNNANNNNNNTTNTNANTNATTANNTNCNNTAATNNNTAANGNNNNNNTNNNNNNTTNNNTNNTNTNNNNTTNNTNTNNNNNNNANNNNNNNTNNNNNNANNNNNTTNNNNNNNANNAANNNGNNANNTNNNAAANNNNNNTTNNTTNNNANNNNNAANNTTNTTTNNAANNNNANNNNANTNNTNNNNNCNTNANNNNNANNTNNNTTNNNTTNNANTNNANTNNNGTNNNNNNNANNNNNTNTNNNNNNNTNNNNTANNNATNNNNNNNNNNNNANNNACNNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNANNNNNNANNNANTNNANTNNNNNTNANNNNNNANNAANNNNNNTTNNNTNNNNNANNANNANNNNNTCNNNNNCANCANNANCANNACNNTCNCNNNNCNNANNNNNAAANNNNAANNNNTCNTCNTCTATGTCAATCAGAGCCATGNGAGATCAAAAAACACTTTCTTACGACAAATCTAATAAACCCTTTTATTCTTCTTCTTCATCTCCATGTTCCTTCACNGCCATCGTCGTCTTCTTGATCTTCGTCTCATATCTCTTATACTCATTCAGTTTCATNTCTTTTCTAAACCCATATTCACCTTCTAAATCCCCCANTTCTCTCCTCGTACCCGTTATCCGTCTCGGGTCGGGTCAAACACCTGAGGAACAAACGGAGCTCAAACACATAGTCTTCGGAATCGCCGCGTCTTCAGATCTATGGAAGCATCGTAGAGAATACGTGAAAACATGGTGGAAACCTAACGGAGTCATGAACGGCGCCGTTTGGCTCGACAAACCCATCAACGACACCGTTTCTTCTTCATCAGCTTTGCCTCAGATCAGAATCTCTTCCGACACGTCGAGTTTCAAGTACCGTTACCGTAACGGTCACAGATCAGCGATCAGGATCACGAGAATCGTGTCCGAGACGGTGANGATGTTAAACGGAACGGAANCGGAGAGAAACGTGAGGTGGGTTGTGATGGGTGACGACGATACCGTGTTTTTCACGGAAAATCTTGTTAGGGTTTTGAGAAAGTACGATCATAAACAGTTTTATTACATCGGAGCTCCGTCCGAGAGTCATTTGCAGAATCTTCATCAGTTTTCTTATGGAATGGCGTACGGTGGAGGAGGGTTTGCTATAAGTTATCCATTAGCCAANGTTTTGGAGAAGATGCAAGATCGGTGTATCGAGAGATACTCCGATTTGTACGGATCCGATGATCGGATTCATGCTTGTATGGCTGAGCTTGGTGTTCCTCTCACTAAAGAAGTTGGATTCCATCAGGTTAGTTTTGTTATTACCGAGAAAAATCAGCCGGAAAAANACNAAGTAAATGTGAAATAGTAAAACTTAAAATCCGTGATTAGATTCATTTGTCATTGACTCTTGTTACTTCACTTACTNATTATTAATTTTATGGTATGGTTTATAACAGTTTGATGTATACGGGAATCTCTTAGGGCTATTATCTGTCCATCCACAAGCACCAATAGTATCNATCCATCACCTAGACGTAGTGGATCCNATATTCCCAAAAACAAACCGAGTCAACGCATTAAAGAAGCTAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_15058516_15058579_oy-0_edi-0_456448_82220_TRUE
SEQUENCE_TARGET=950,163
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8511168_8511551_po-0_wu-0_57728_28469_TRUE
SEQUENCE_TARGET=950,483
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGANGGGANGGNGGTTTCCCTATGTNTTGACGAAAAATGTACAGTTTAAAACCTTTCTCTCAGTCTGTTGGTTGGCTCCATTCGCAGCTNACTNTTTTCGTCAACCNAATTNTGTTTATCCATTTCTTNAATCATCACTTAATNANTGCCTAGTTCTAATCCCAAAGTTGGATCAGAAATCCAAATTTCAAAGTCGNTATACAGAGCCGTAGCTTTCTGGCAATTTTGATTCCCACCAAAAAGGTGATCACTTTATATTNGATTCCNCNTCTTTTCTNCTATTAATTTTGACCCATTCGCTTCTTATCACTCTCACANNNNAAANAAAAANACCCATGAAAATCCACCCATTACCCAGAAACGAAAATAACAACAATCTGATCCATCACGCTCGTGATCCGACCCGAGAACCCGGAAAGAAGCTCCGTCGTCTCCCACATATCTTCAGCCGTGTTCTAGAGCTNCCCTTGAAGTCAGACGCTGACGTGGCTGTTGAAGAGAGTCATGACTGCTTCAGGTTNGTNGCGGAAACAGACGGNGGAGGAGGAGGAGGTGTGAGGGCTTACATGGTTGAGATTCATCCTGGTGTGACCAAGATTCTTGTGAGAACCAATGGTTCATCGTCTCTTGGTTTGTCGCTTGATGAGCTTGAGCTTGACGTGTGGCGTTTCAGGNTACCGGAATCAACTAGGCCTGAGCTCGTTACCGTGGATTGCGACGGCGATGGAGAATTGATTGTCACTGTGCCTAAGATTGAAGACAATGGTAGAGATTTGANAGTGCTTGTACAGTAATCTAATGTGTTTGTGATCAAATGAGAAAAATCTTGNCTTTTTTTATCTAATGGTTATCAAAGTNAAGATCTTTTTGTTTCTTTATGAAAATATGGATACAGAGTAGGTTNTGCCTTATGTAACGTAACATCAATTGGTTTTAGTTTTAGAGATAAAATCCTTTGAGAGGGGCAAATTTATTGAGGATCTCTGATTCACGTTNAAGAGCATTGTCTTTCTTCATTTCCCATTCAGGTTTCTTCAAGTTAGCTGCTTGANCTTCTTGAACCACTTTAGGGATTGAGTTCGCTACCATCTTCTGACCAAAGATTAGATCAACCTTCTCTTTCCCCTTGTTACTGATGCAGCGGGTAAGCTCGGTTCGTTTAGCTTGAAGCTGAGCGATTTGCTCGTCAATNNNACGGATTGTCTCTGAGTTTCTATNGACTTCCGTTTGGATATTGCCTAGCTTGTCCTTTAAGTCGCTGTATTCGTACTTGAGAGATGCGACCTTCGCTTTGTTAAGCTCGAGAGCGGAGAAGAACCTATCTGCTTCTACTATCATGCTTTGGTTCTCTTGAAAAACTTCGCTGAAAGTTGGAATTTCTTCGATTAGCTTCAACCTCACTATTTNTTCAGCTGATAGGCTTGGATCTTTCTTGANTTTAGCTGCCAAGCTTGCCATCTCCGTAGCTTCATTAGACATGAACAAGGAAGAGAAATCTTTCTCCAGAAGCGATGAAAGCTTCATTCGAGCTTCTGTCCATTCGTAATCTCTCACTGGAGAGCTTGCACTAANGGGAAGTGATGATGAAGACTCCTGTCCTGCTTCTGAAGGCTGTTGCTGAAGAAGATTCTCTAGAGAGTTCAGATAACTGTCAAACTCTTCTGGGTTTATATCCACAGGAGGGTGAGAGACTGCAATGACACCGTTCATAGAAGAAACATAGATATGCGGAACAAAAGCCNNNTCTTCTTCTTCTTCGTCTGCATAGTTACCTCTGCGTTTCTTCATCCTCTTGCTGCCTTTCTTTGCANCTGATTCCTTNTTTTCTTCAGTCAAGTCAAACACATCACCTTGTTGCTGTCTTGTGTANCGTNTCGAGGAAGATTCCCNAGCTGACTTATGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.349987
PRIMER_LEFT_0_PENALTY=0.205314
PRIMER_RIGHT_0_PENALTY=0.144674
PRIMER_LEFT_0_SEQUENCE=CCCATTACCCAGAAACGAAA
PRIMER_RIGHT_0_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_0=826,20
PRIMER_RIGHT_0=1782,20
PRIMER_LEFT_0_TM=59.795
PRIMER_RIGHT_0_TM=60.145
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=957
PRIMER_PAIR_1_PENALTY=0.484849
PRIMER_LEFT_1_PENALTY=0.205314
PRIMER_RIGHT_1_PENALTY=0.279536
PRIMER_LEFT_1_SEQUENCE=CCCATTACCCAGAAACGAAA
PRIMER_RIGHT_1_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_1=826,20
PRIMER_RIGHT_1=1626,20
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=60.280
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=801
PRIMER_PAIR_2_PENALTY=0.497743
PRIMER_LEFT_2_PENALTY=0.353069
PRIMER_RIGHT_2_PENALTY=0.144674
PRIMER_LEFT_2_SEQUENCE=TCCACCCATTACCCAGAAAC
PRIMER_RIGHT_2_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_2=822,20
PRIMER_RIGHT_2=1782,20
PRIMER_LEFT_2_TM=59.647
PRIMER_RIGHT_2_TM=60.145
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=961
PRIMER_PAIR_3_PENALTY=0.507127
PRIMER_LEFT_3_PENALTY=0.227592
PRIMER_RIGHT_3_PENALTY=0.279536
PRIMER_LEFT_3_SEQUENCE=TTTGATTCCCACCAAAAAGG
PRIMER_RIGHT_3_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_3=704,20
PRIMER_RIGHT_3=1626,20
PRIMER_LEFT_3_TM=59.772
PRIMER_RIGHT_3_TM=60.280
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=923
PRIMER_PAIR_4_PENALTY=0.511463
PRIMER_LEFT_4_PENALTY=0.205314
PRIMER_RIGHT_4_PENALTY=0.306150
PRIMER_LEFT_4_SEQUENCE=CCCATTACCCAGAAACGAAA
PRIMER_RIGHT_4_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_4=826,20
PRIMER_RIGHT_4=1567,20
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=59.694
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=742
PRIMER_PAIR_5_PENALTY=0.512371
PRIMER_LEFT_5_PENALTY=0.232835
PRIMER_RIGHT_5_PENALTY=0.279536
PRIMER_LEFT_5_SEQUENCE=GATTCCCACCAAAAAGGTGA
PRIMER_RIGHT_5_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_5=707,20
PRIMER_RIGHT_5=1626,20
PRIMER_LEFT_5_TM=59.767
PRIMER_RIGHT_5_TM=60.280
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=920
PRIMER_PAIR_6_PENALTY=0.533741
PRIMER_LEFT_6_PENALTY=0.227592
PRIMER_RIGHT_6_PENALTY=0.306150
PRIMER_LEFT_6_SEQUENCE=TTTGATTCCCACCAAAAAGG
PRIMER_RIGHT_6_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_6=704,20
PRIMER_RIGHT_6=1567,20
PRIMER_LEFT_6_TM=59.772
PRIMER_RIGHT_6_TM=59.694
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=864
PRIMER_PAIR_7_PENALTY=0.538985
PRIMER_LEFT_7_PENALTY=0.232835
PRIMER_RIGHT_7_PENALTY=0.306150
PRIMER_LEFT_7_SEQUENCE=GATTCCCACCAAAAAGGTGA
PRIMER_RIGHT_7_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_7=707,20
PRIMER_RIGHT_7=1567,20
PRIMER_LEFT_7_TM=59.767
PRIMER_RIGHT_7_TM=59.694
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=861
PRIMER_PAIR_8_PENALTY=0.594506
PRIMER_LEFT_8_PENALTY=0.314970
PRIMER_RIGHT_8_PENALTY=0.279536
PRIMER_LEFT_8_SEQUENCE=TTTTGACCCATTCGCTTCTT
PRIMER_RIGHT_8_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_8=765,20
PRIMER_RIGHT_8=1626,20
PRIMER_LEFT_8_TM=59.685
PRIMER_RIGHT_8_TM=60.280
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=862
PRIMER_PAIR_9_PENALTY=0.621120
PRIMER_LEFT_9_PENALTY=0.314970
PRIMER_RIGHT_9_PENALTY=0.306150
PRIMER_LEFT_9_SEQUENCE=TTTTGACCCATTCGCTTCTT
PRIMER_RIGHT_9_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_9=765,20
PRIMER_RIGHT_9=1567,20
PRIMER_LEFT_9_TM=59.685
PRIMER_RIGHT_9_TM=59.694
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=803
PRIMER_PAIR_10_PENALTY=0.627335
PRIMER_LEFT_10_PENALTY=0.482662
PRIMER_RIGHT_10_PENALTY=0.144674
PRIMER_LEFT_10_SEQUENCE=TCCCACATATCTTCAGCCGT
PRIMER_RIGHT_10_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_10=912,20
PRIMER_RIGHT_10=1782,20
PRIMER_LEFT_10_TM=60.483
PRIMER_RIGHT_10_TM=60.145
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.1000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=871
PRIMER_PAIR_11_PENALTY=0.632605
PRIMER_LEFT_11_PENALTY=0.353069
PRIMER_RIGHT_11_PENALTY=0.279536
PRIMER_LEFT_11_SEQUENCE=TCCACCCATTACCCAGAAAC
PRIMER_RIGHT_11_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_11=822,20
PRIMER_RIGHT_11=1626,20
PRIMER_LEFT_11_TM=59.647
PRIMER_RIGHT_11_TM=60.280
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=805
PRIMER_PAIR_12_PENALTY=0.635966
PRIMER_LEFT_12_PENALTY=0.205314
PRIMER_RIGHT_12_PENALTY=0.430652
PRIMER_LEFT_12_SEQUENCE=CCCATTACCCAGAAACGAAA
PRIMER_RIGHT_12_SEQUENCE=GCTAGGCAATATCCAAACGG
PRIMER_LEFT_12=826,20
PRIMER_RIGHT_12=1722,20
PRIMER_LEFT_12_TM=59.795
PRIMER_RIGHT_12_TM=59.569
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.0000
PRIMER_RIGHT_12_END_STABILITY=9.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=897
PRIMER_PAIR_13_PENALTY=0.640742
PRIMER_LEFT_13_PENALTY=0.361206
PRIMER_RIGHT_13_PENALTY=0.279536
PRIMER_LEFT_13_SEQUENCE=ACAGAGCCGTAGCTTTCTGG
PRIMER_RIGHT_13_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_13=680,20
PRIMER_RIGHT_13=1626,20
PRIMER_LEFT_13_TM=59.639
PRIMER_RIGHT_13_TM=60.280
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=947
PRIMER_PAIR_14_PENALTY=0.659219
PRIMER_LEFT_14_PENALTY=0.353069
PRIMER_RIGHT_14_PENALTY=0.306150
PRIMER_LEFT_14_SEQUENCE=TCCACCCATTACCCAGAAAC
PRIMER_RIGHT_14_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_14=822,20
PRIMER_RIGHT_14=1567,20
PRIMER_LEFT_14_TM=59.647
PRIMER_RIGHT_14_TM=59.694
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=9.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=746
PRIMER_PAIR_15_PENALTY=0.663431
PRIMER_LEFT_15_PENALTY=0.518757
PRIMER_RIGHT_15_PENALTY=0.144674
PRIMER_LEFT_15_SEQUENCE=GAAAATCCACCCATTACCCA
PRIMER_RIGHT_15_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_15=817,20
PRIMER_RIGHT_15=1782,20
PRIMER_LEFT_15_TM=59.481
PRIMER_RIGHT_15_TM=60.145
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=966
PRIMER_PAIR_16_PENALTY=0.663431
PRIMER_LEFT_16_PENALTY=0.518757
PRIMER_RIGHT_16_PENALTY=0.144674
PRIMER_LEFT_16_SEQUENCE=TGAAAATCCACCCATTACCC
PRIMER_RIGHT_16_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_16=816,20
PRIMER_RIGHT_16=1782,20
PRIMER_LEFT_16_TM=59.481
PRIMER_RIGHT_16_TM=60.145
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=967
PRIMER_PAIR_17_PENALTY=0.667356
PRIMER_LEFT_17_PENALTY=0.361206
PRIMER_RIGHT_17_PENALTY=0.306150
PRIMER_LEFT_17_SEQUENCE=ACAGAGCCGTAGCTTTCTGG
PRIMER_RIGHT_17_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_17=680,20
PRIMER_RIGHT_17=1567,20
PRIMER_LEFT_17_TM=59.639
PRIMER_RIGHT_17_TM=59.694
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=888
PRIMER_PAIR_18_PENALTY=0.745622
PRIMER_LEFT_18_PENALTY=0.314970
PRIMER_RIGHT_18_PENALTY=0.430652
PRIMER_LEFT_18_SEQUENCE=TTTTGACCCATTCGCTTCTT
PRIMER_RIGHT_18_SEQUENCE=GCTAGGCAATATCCAAACGG
PRIMER_LEFT_18=765,20
PRIMER_RIGHT_18=1722,20
PRIMER_LEFT_18_TM=59.685
PRIMER_RIGHT_18_TM=59.569
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=958
PRIMER_PAIR_19_PENALTY=0.762197
PRIMER_LEFT_19_PENALTY=0.482662
PRIMER_RIGHT_19_PENALTY=0.279536
PRIMER_LEFT_19_SEQUENCE=TCCCACATATCTTCAGCCGT
PRIMER_RIGHT_19_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_19=912,20
PRIMER_RIGHT_19=1626,20
PRIMER_LEFT_19_TM=60.483
PRIMER_RIGHT_19_TM=60.280
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=11.1000
PRIMER_RIGHT_19_END_STABILITY=6.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=715
PRIMER_PAIR_20_PENALTY=0.783721
PRIMER_LEFT_20_PENALTY=0.353069
PRIMER_RIGHT_20_PENALTY=0.430652
PRIMER_LEFT_20_SEQUENCE=TCCACCCATTACCCAGAAAC
PRIMER_RIGHT_20_SEQUENCE=GCTAGGCAATATCCAAACGG
PRIMER_LEFT_20=822,20
PRIMER_RIGHT_20=1722,20
PRIMER_LEFT_20_TM=59.647
PRIMER_RIGHT_20_TM=59.569
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=9.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=901
PRIMER_PAIR_21_PENALTY=0.788811
PRIMER_LEFT_21_PENALTY=0.482662
PRIMER_RIGHT_21_PENALTY=0.306150
PRIMER_LEFT_21_SEQUENCE=TCCCACATATCTTCAGCCGT
PRIMER_RIGHT_21_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_21=912,20
PRIMER_RIGHT_21=1567,20
PRIMER_LEFT_21_TM=60.483
PRIMER_RIGHT_21_TM=59.694
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=11.1000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=656
PRIMER_PAIR_22_PENALTY=0.798293
PRIMER_LEFT_22_PENALTY=0.518757
PRIMER_RIGHT_22_PENALTY=0.279536
PRIMER_LEFT_22_SEQUENCE=GAAAATCCACCCATTACCCA
PRIMER_RIGHT_22_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_22=817,20
PRIMER_RIGHT_22=1626,20
PRIMER_LEFT_22_TM=59.481
PRIMER_RIGHT_22_TM=60.280
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=6.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=810
PRIMER_PAIR_23_PENALTY=0.798293
PRIMER_LEFT_23_PENALTY=0.518757
PRIMER_RIGHT_23_PENALTY=0.279536
PRIMER_LEFT_23_SEQUENCE=TGAAAATCCACCCATTACCC
PRIMER_RIGHT_23_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_23=816,20
PRIMER_RIGHT_23=1626,20
PRIMER_LEFT_23_TM=59.481
PRIMER_RIGHT_23_TM=60.280
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=6.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=811
PRIMER_PAIR_24_PENALTY=0.824907
PRIMER_LEFT_24_PENALTY=0.518757
PRIMER_RIGHT_24_PENALTY=0.306150
PRIMER_LEFT_24_SEQUENCE=GAAAATCCACCCATTACCCA
PRIMER_RIGHT_24_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_24=817,20
PRIMER_RIGHT_24=1567,20
PRIMER_LEFT_24_TM=59.481
PRIMER_RIGHT_24_TM=59.694
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=751
PRIMER_PAIR_25_PENALTY=0.824907
PRIMER_LEFT_25_PENALTY=0.518757
PRIMER_RIGHT_25_PENALTY=0.306150
PRIMER_LEFT_25_SEQUENCE=TGAAAATCCACCCATTACCC
PRIMER_RIGHT_25_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_25=816,20
PRIMER_RIGHT_25=1567,20
PRIMER_LEFT_25_TM=59.481
PRIMER_RIGHT_25_TM=59.694
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=752
PRIMER_PAIR_26_PENALTY=0.853736
PRIMER_LEFT_26_PENALTY=0.205314
PRIMER_RIGHT_26_PENALTY=0.648422
PRIMER_LEFT_26_SEQUENCE=CCCATTACCCAGAAACGAAA
PRIMER_RIGHT_26_SEQUENCE=CCGCTCTCGAGCTTAACAAA
PRIMER_LEFT_26=826,20
PRIMER_RIGHT_26=1789,20
PRIMER_LEFT_26_TM=59.795
PRIMER_RIGHT_26_TM=60.648
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.0000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=964
PRIMER_PAIR_27_PENALTY=0.856393
PRIMER_LEFT_27_PENALTY=0.711720
PRIMER_RIGHT_27_PENALTY=0.144674
PRIMER_LEFT_27_SEQUENCE=ACCCATTACCCAGAAACGAA
PRIMER_RIGHT_27_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_27=825,20
PRIMER_RIGHT_27=1782,20
PRIMER_LEFT_27_TM=59.288
PRIMER_RIGHT_27_TM=60.145
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=958
PRIMER_PAIR_28_PENALTY=0.862407
PRIMER_LEFT_28_PENALTY=0.205314
PRIMER_RIGHT_28_PENALTY=0.657094
PRIMER_LEFT_28_SEQUENCE=CCCATTACCCAGAAACGAAA
PRIMER_RIGHT_28_SEQUENCE=GCTGCATCAGTAACAAGGGG
PRIMER_LEFT_28=826,20
PRIMER_RIGHT_28=1621,20
PRIMER_LEFT_28_TM=59.795
PRIMER_RIGHT_28_TM=60.657
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.0000
PRIMER_RIGHT_28_END_STABILITY=10.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=796
PRIMER_PAIR_29_PENALTY=0.884685
PRIMER_LEFT_29_PENALTY=0.227592
PRIMER_RIGHT_29_PENALTY=0.657094
PRIMER_LEFT_29_SEQUENCE=TTTGATTCCCACCAAAAAGG
PRIMER_RIGHT_29_SEQUENCE=GCTGCATCAGTAACAAGGGG
PRIMER_LEFT_29=704,20
PRIMER_RIGHT_29=1621,20
PRIMER_LEFT_29_TM=59.772
PRIMER_RIGHT_29_TM=60.657
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=10.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=918
PRIMER_PAIR_30_PENALTY=0.889598
PRIMER_LEFT_30_PENALTY=0.744925
PRIMER_RIGHT_30_PENALTY=0.144674
PRIMER_LEFT_30_SEQUENCE=CACCCATTACCCAGAAACGA
PRIMER_RIGHT_30_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_30=824,20
PRIMER_RIGHT_30=1782,20
PRIMER_LEFT_30_TM=60.745
PRIMER_RIGHT_30_TM=60.145
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.4000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=959
PRIMER_PAIR_31_PENALTY=0.889929
PRIMER_LEFT_31_PENALTY=0.232835
PRIMER_RIGHT_31_PENALTY=0.657094
PRIMER_LEFT_31_SEQUENCE=GATTCCCACCAAAAAGGTGA
PRIMER_RIGHT_31_SEQUENCE=GCTGCATCAGTAACAAGGGG
PRIMER_LEFT_31=707,20
PRIMER_RIGHT_31=1621,20
PRIMER_LEFT_31_TM=59.767
PRIMER_RIGHT_31_TM=60.657
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=10.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=915
PRIMER_PAIR_32_PENALTY=0.949409
PRIMER_LEFT_32_PENALTY=0.518757
PRIMER_RIGHT_32_PENALTY=0.430652
PRIMER_LEFT_32_SEQUENCE=GAAAATCCACCCATTACCCA
PRIMER_RIGHT_32_SEQUENCE=GCTAGGCAATATCCAAACGG
PRIMER_LEFT_32=817,20
PRIMER_RIGHT_32=1722,20
PRIMER_LEFT_32_TM=59.481
PRIMER_RIGHT_32_TM=59.569
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=906
PRIMER_PAIR_33_PENALTY=0.949409
PRIMER_LEFT_33_PENALTY=0.518757
PRIMER_RIGHT_33_PENALTY=0.430652
PRIMER_LEFT_33_SEQUENCE=TGAAAATCCACCCATTACCC
PRIMER_RIGHT_33_SEQUENCE=GCTAGGCAATATCCAAACGG
PRIMER_LEFT_33=816,20
PRIMER_RIGHT_33=1722,20
PRIMER_LEFT_33_TM=59.481
PRIMER_RIGHT_33_TM=59.569
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=9.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=907
PRIMER_PAIR_34_PENALTY=0.972064
PRIMER_LEFT_34_PENALTY=0.314970
PRIMER_RIGHT_34_PENALTY=0.657094
PRIMER_LEFT_34_SEQUENCE=TTTTGACCCATTCGCTTCTT
PRIMER_RIGHT_34_SEQUENCE=GCTGCATCAGTAACAAGGGG
PRIMER_LEFT_34=765,20
PRIMER_RIGHT_34=1621,20
PRIMER_LEFT_34_TM=59.685
PRIMER_RIGHT_34_TM=60.657
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=10.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=857
PRIMER_PAIR_35_PENALTY=0.978753
PRIMER_LEFT_35_PENALTY=0.834079
PRIMER_RIGHT_35_PENALTY=0.144674
PRIMER_LEFT_35_SEQUENCE=GAAAGAAGCTCCGTCGTCTC
PRIMER_RIGHT_35_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_35=894,20
PRIMER_RIGHT_35=1782,20
PRIMER_LEFT_35_TM=59.166
PRIMER_RIGHT_35_TM=60.145
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.1000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=889
PRIMER_PAIR_36_PENALTY=0.980519
PRIMER_LEFT_36_PENALTY=0.205314
PRIMER_RIGHT_36_PENALTY=0.775205
PRIMER_LEFT_36_SEQUENCE=CCCATTACCCAGAAACGAAA
PRIMER_RIGHT_36_SEQUENCE=TAAACGAACCGAGCTTACCC
PRIMER_LEFT_36=826,20
PRIMER_RIGHT_36=1641,20
PRIMER_LEFT_36_TM=59.795
PRIMER_RIGHT_36_TM=59.225
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.0000
PRIMER_RIGHT_36_END_STABILITY=8.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=816
PRIMER_PAIR_37_PENALTY=0.991255
PRIMER_LEFT_37_PENALTY=0.711720
PRIMER_RIGHT_37_PENALTY=0.279536
PRIMER_LEFT_37_SEQUENCE=ACCCATTACCCAGAAACGAA
PRIMER_RIGHT_37_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_37=825,20
PRIMER_RIGHT_37=1626,20
PRIMER_LEFT_37_TM=59.288
PRIMER_RIGHT_37_TM=60.280
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=6.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=802
PRIMER_PAIR_38_PENALTY=1.001491
PRIMER_LEFT_38_PENALTY=0.353069
PRIMER_RIGHT_38_PENALTY=0.648422
PRIMER_LEFT_38_SEQUENCE=TCCACCCATTACCCAGAAAC
PRIMER_RIGHT_38_SEQUENCE=CCGCTCTCGAGCTTAACAAA
PRIMER_LEFT_38=822,20
PRIMER_RIGHT_38=1789,20
PRIMER_LEFT_38_TM=59.647
PRIMER_RIGHT_38_TM=60.648
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=968
PRIMER_PAIR_39_PENALTY=1.002797
PRIMER_LEFT_39_PENALTY=0.227592
PRIMER_RIGHT_39_PENALTY=0.775205
PRIMER_LEFT_39_SEQUENCE=TTTGATTCCCACCAAAAAGG
PRIMER_RIGHT_39_SEQUENCE=TAAACGAACCGAGCTTACCC
PRIMER_LEFT_39=704,20
PRIMER_RIGHT_39=1641,20
PRIMER_LEFT_39_TM=59.772
PRIMER_RIGHT_39_TM=59.225
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=8.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=938
PRIMER_PAIR_40_PENALTY=1.008040
PRIMER_LEFT_40_PENALTY=0.232835
PRIMER_RIGHT_40_PENALTY=0.775205
PRIMER_LEFT_40_SEQUENCE=GATTCCCACCAAAAAGGTGA
PRIMER_RIGHT_40_SEQUENCE=TAAACGAACCGAGCTTACCC
PRIMER_LEFT_40=707,20
PRIMER_RIGHT_40=1641,20
PRIMER_LEFT_40_TM=59.767
PRIMER_RIGHT_40_TM=59.225
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=935
PRIMER_PAIR_41_PENALTY=1.010163
PRIMER_LEFT_41_PENALTY=0.353069
PRIMER_RIGHT_41_PENALTY=0.657094
PRIMER_LEFT_41_SEQUENCE=TCCACCCATTACCCAGAAAC
PRIMER_RIGHT_41_SEQUENCE=GCTGCATCAGTAACAAGGGG
PRIMER_LEFT_41=822,20
PRIMER_RIGHT_41=1621,20
PRIMER_LEFT_41_TM=59.647
PRIMER_RIGHT_41_TM=60.657
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=10.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=800
PRIMER_PAIR_42_PENALTY=1.015130
PRIMER_LEFT_42_PENALTY=0.735594
PRIMER_RIGHT_42_PENALTY=0.279536
PRIMER_LEFT_42_SEQUENCE=TTGATTCCCACCAAAAAGGT
PRIMER_RIGHT_42_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_42=705,20
PRIMER_RIGHT_42=1626,20
PRIMER_LEFT_42_TM=59.264
PRIMER_RIGHT_42_TM=60.280
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=922
PRIMER_PAIR_43_PENALTY=1.017869
PRIMER_LEFT_43_PENALTY=0.711720
PRIMER_RIGHT_43_PENALTY=0.306150
PRIMER_LEFT_43_SEQUENCE=ACCCATTACCCAGAAACGAA
PRIMER_RIGHT_43_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_43=825,20
PRIMER_RIGHT_43=1567,20
PRIMER_LEFT_43_TM=59.288
PRIMER_RIGHT_43_TM=59.694
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=8.4000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=743
PRIMER_PAIR_44_PENALTY=1.018300
PRIMER_LEFT_44_PENALTY=0.361206
PRIMER_RIGHT_44_PENALTY=0.657094
PRIMER_LEFT_44_SEQUENCE=ACAGAGCCGTAGCTTTCTGG
PRIMER_RIGHT_44_SEQUENCE=GCTGCATCAGTAACAAGGGG
PRIMER_LEFT_44=680,20
PRIMER_RIGHT_44=1621,20
PRIMER_LEFT_44_TM=59.639
PRIMER_RIGHT_44_TM=60.657
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=10.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=942
PRIMER_PAIR_45_PENALTY=1.024460
PRIMER_LEFT_45_PENALTY=0.744925
PRIMER_RIGHT_45_PENALTY=0.279536
PRIMER_LEFT_45_SEQUENCE=CACCCATTACCCAGAAACGA
PRIMER_RIGHT_45_SEQUENCE=TACCCGCTGCATCAGTAACA
PRIMER_LEFT_45=824,20
PRIMER_RIGHT_45=1626,20
PRIMER_LEFT_45_TM=60.745
PRIMER_RIGHT_45_TM=60.280
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.4000
PRIMER_RIGHT_45_END_STABILITY=6.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=803
PRIMER_PAIR_46_PENALTY=1.041744
PRIMER_LEFT_46_PENALTY=0.735594
PRIMER_RIGHT_46_PENALTY=0.306150
PRIMER_LEFT_46_SEQUENCE=TTGATTCCCACCAAAAAGGT
PRIMER_RIGHT_46_SEQUENCE=TGGTAGCGAACTCAATCCCT
PRIMER_LEFT_46=705,20
PRIMER_RIGHT_46=1567,20
PRIMER_LEFT_46_TM=59.264
PRIMER_RIGHT_46_TM=59.694
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=863
PRIMER_PAIR_47_PENALTY=1.043708
PRIMER_LEFT_47_PENALTY=0.899034
PRIMER_RIGHT_47_PENALTY=0.144674
PRIMER_LEFT_47_SEQUENCE=ATCCACCCATTACCCAGAAA
PRIMER_RIGHT_47_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_47=821,20
PRIMER_RIGHT_47=1782,20
PRIMER_LEFT_47_TM=59.101
PRIMER_RIGHT_47_TM=60.145
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=962
PRIMER_PAIR_48_PENALTY=1.043708
PRIMER_LEFT_48_PENALTY=0.899034
PRIMER_RIGHT_48_PENALTY=0.144674
PRIMER_LEFT_48_SEQUENCE=AATCCACCCATTACCCAGAA
PRIMER_RIGHT_48_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_48=820,20
PRIMER_RIGHT_48=1782,20
PRIMER_LEFT_48_TM=59.101
PRIMER_RIGHT_48_TM=60.145
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=963
PRIMER_PAIR_49_PENALTY=1.043708
PRIMER_LEFT_49_PENALTY=0.899034
PRIMER_RIGHT_49_PENALTY=0.144674
PRIMER_LEFT_49_SEQUENCE=AAATCCACCCATTACCCAGA
PRIMER_RIGHT_49_SEQUENCE=CGAGCTTAACAAAGCGAAGG
PRIMER_LEFT_49=819,20
PRIMER_RIGHT_49=1782,20
PRIMER_LEFT_49_TM=59.101
PRIMER_RIGHT_49_TM=60.145
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=964
=
SEQUENCE_ID=MAGIC.446_5_18767254_18767346_po-0_bur-0_136024_10245_TRUE
SEQUENCE_TARGET=950,192
SEQUENCE_TEMPLATE=TTAGAAGNTNNATACCTTCACTCCATCCCATTGTTTCCCATCACTCTCTTCTTTTGATAAGCTAAGCTCAACAAGATTCTCGAGGTTAGGGAATTCTTCAATGTTTGTTCCAAANAGNNNGAGAACGGAGATGTTGGTTGAGAATTCGGGAAAAGTCCTCAACTCCNAGCAATATCGGAAGTTGAGGTGGTCGANAGATTTNAGGTTGAAGCCGNTTGGGAGAATTTCCAGACTATNGCAGAATNCCATGTCCAATTTCAANAGTTTATTGAGATTTCNTATAGAGGANGGAAGCTCCACCAAACTCTTNCAAAAACNAAGTTTAAGTNTCTCNAGATTAGTAGNCATNGAAAGATCTGGAATTNCTTTNAGGNTTGATGATCCAANCANATCCATTTCCTTTAGGCATGTAAGCGACTNTACTCAATAAAANTTAAACACANTGCTTAGTANAAATTACACATCACGTGANATATTGTCATNCAAANATTANAATACTTACCGCAACTCCNTCCCACAGCTTATGTANCTTGCTATTCGGCATTTTGAGCGTNACAAGNTTTTCAGGACGAAAATTANAAGGCATACANCTCATNGGAAAATTGGGCCAACANAATAGTTTGAGTCTAGGGGGNAANTAGTCCAAACTTTNNGGTAAGTACAATCTNCCTGCTTTTCCAAAGTTCTTNGAATCAATTTCTAAGAAACGGAGATNACNCATCCCTTTGAANGCACTCTCATGTACATNCAACTCATCAATNTCACNTGTATTTAAAGATATACCTAAAACNTTTTGAGTACCCTGNNNAAAAATATGATATTAATGAGAAANAGACAAANCCATACAATTCATCATTTATCGGATGACATTCTCTTAACCTCACATACTGTGTNNNNNNNNNNNNNNNNNNNNNNNAAAAGAGAACTTACAATGNCTTCACTGAGTACATCANAAATATCATTTGGATCCACCAGAAATTCTCGTTTCCCAGGCTTGTCANTGGACTGTGTGCGAACAATTTTTCTNCCCATTTCTTGTANCATACGGTGCATCTCCACANNACCCCGTCTGACATGAATGATGGACTTATCNACNAGGTTTTTNANCCCAATATTAATACCCAAGTCTGNGAGCAACGACGTGATNGNTGTGACTTCCATATGATTGAAAANACATGCNATATGACGAAATATCGCTTTATCNTCTTCGCTACCTAANCCATCATAGCTGATTCTTAGTATTTTCTCAATNTTATCGTGTNGACCATTCTCAAGCCTTGGCAGCATTTCCATCCANTACTCCTTATCCNNNCCCCTCAAATATGAACCCAAAACATTAAGACCCAAAGGAAGACTATCCACATGTCTTGCAACTTGAACTACAAGCATCTCAAAACCTTCTGGTGNAGATTTTTTCCTAAAAGCAGATTGACATAACATTGCAAGAGCGTGTTNCTGAGTTGGGAGACTAACTTCATAAATATGATCAATCNCATGAGCCCTTAAGAAATGCTTATTATTTGTAACCGCAATGATNNTGCTCCCACTTCCAAACCATTGAGTTTGACCCACCAANGAATCTAAGATCACTTGGTCATCCAAATCATCAACGATAATAAGAACTTTTTGGTGTTGTAGCCTCTCTCCTAGTACACCTANNTGNTCNATCTTTATGTCNGGCANNNTNAGAATTTCNGAGAGGAGTTTCTCTTGTAAATGTAACTTCATGTTGTGGTCGTCNGGATTGGCTCTACTAAAAATTTCTCTACTCTTATATACAAAAGCTCTGTCTATGAATTTGCTAACTNGGAAATGCCGAGAAAGTTGGTTAAACAGAGCTCTTGCAATGGTAGTCTTACCAATCCCCGAGGAACCCCATATACCAACCATTCTCACTTCCTCAGCTTCCAATTTCAACAATACACTCATATTTGCAATATGATCTTCGATGCCAACANAATTTTCAAAGTCCTTGGGTGTNGTTAAAAGCAGTTTAGNCAAAACATCATTGGCTATTTCTTCAATCATTTTTGCTTCGTCATCCCTAATAANGNTAAGGACAATTTTAAAGNNNNNNNNNNAANNNNATCTN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.613639
PRIMER_LEFT_0_PENALTY=0.230329
PRIMER_RIGHT_0_PENALTY=0.383310
PRIMER_LEFT_0_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_0_SEQUENCE=TTGGGTTCATATTTGAGGGG
PRIMER_LEFT_0=533,20
PRIMER_RIGHT_0=1331,20
PRIMER_LEFT_0_TM=60.230
PRIMER_RIGHT_0_TM=59.617
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=10.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=799
PRIMER_PAIR_1_PENALTY=1.471781
PRIMER_LEFT_1_PENALTY=1.088471
PRIMER_RIGHT_1_PENALTY=0.383310
PRIMER_LEFT_1_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_1_SEQUENCE=TTGGGTTCATATTTGAGGGG
PRIMER_LEFT_1=530,20
PRIMER_RIGHT_1=1331,20
PRIMER_LEFT_1_TM=58.912
PRIMER_RIGHT_1_TM=59.617
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=802
PRIMER_PAIR_2_PENALTY=1.471781
PRIMER_LEFT_2_PENALTY=1.088471
PRIMER_RIGHT_2_PENALTY=0.383310
PRIMER_LEFT_2_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_2_SEQUENCE=TTGGGTTCATATTTGAGGGG
PRIMER_LEFT_2=529,20
PRIMER_RIGHT_2=1331,20
PRIMER_LEFT_2_TM=58.912
PRIMER_RIGHT_2_TM=59.617
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=803
PRIMER_PAIR_3_PENALTY=1.522380
PRIMER_LEFT_3_PENALTY=1.139070
PRIMER_RIGHT_3_PENALTY=0.383310
PRIMER_LEFT_3_SEQUENCE=CTTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_3_SEQUENCE=TTGGGTTCATATTTGAGGGG
PRIMER_LEFT_3=529,21
PRIMER_RIGHT_3=1331,20
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=59.617
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=803
PRIMER_PAIR_4_PENALTY=1.524281
PRIMER_LEFT_4_PENALTY=0.230329
PRIMER_RIGHT_4_PENALTY=1.293952
PRIMER_LEFT_4_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_4_SEQUENCE=TCAAGTTGCAAGACATGTGGA
PRIMER_LEFT_4=533,20
PRIMER_RIGHT_4=1377,21
PRIMER_LEFT_4_TM=60.230
PRIMER_RIGHT_4_TM=60.294
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=845
PRIMER_PAIR_5_PENALTY=1.984159
PRIMER_LEFT_5_PENALTY=0.230329
PRIMER_RIGHT_5_PENALTY=1.753830
PRIMER_LEFT_5_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_5_SEQUENCE=CAAGTTGCAAGACATGTGGA
PRIMER_LEFT_5=533,20
PRIMER_RIGHT_5=1376,20
PRIMER_LEFT_5_TM=60.230
PRIMER_RIGHT_5_TM=58.246
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=844
PRIMER_PAIR_6_PENALTY=2.008703
PRIMER_LEFT_6_PENALTY=1.625393
PRIMER_RIGHT_6_PENALTY=0.383310
PRIMER_LEFT_6_SEQUENCE=TCCTTTAGGCATGTAAGCGAC
PRIMER_RIGHT_6_SEQUENCE=TTGGGTTCATATTTGAGGGG
PRIMER_LEFT_6=397,21
PRIMER_RIGHT_6=1331,20
PRIMER_LEFT_6_TM=59.375
PRIMER_RIGHT_6_TM=59.617
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=10.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=935
PRIMER_PAIR_7_PENALTY=2.117717
PRIMER_LEFT_7_PENALTY=0.230329
PRIMER_RIGHT_7_PENALTY=1.887388
PRIMER_LEFT_7_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_7_SEQUENCE=TTTGGGTTCATATTTGAGGGG
PRIMER_LEFT_7=533,20
PRIMER_RIGHT_7=1332,21
PRIMER_LEFT_7_TM=60.230
PRIMER_RIGHT_7_TM=60.887
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=10.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=800
PRIMER_PAIR_8_PENALTY=2.191984
PRIMER_LEFT_8_PENALTY=0.230329
PRIMER_RIGHT_8_PENALTY=1.961655
PRIMER_LEFT_8_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_8_SEQUENCE=TGTGGATAGTCTTCCTTTGGG
PRIMER_LEFT_8=533,20
PRIMER_RIGHT_8=1362,21
PRIMER_LEFT_8_TM=60.230
PRIMER_RIGHT_8_TM=59.038
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=830
PRIMER_PAIR_9_PENALTY=2.282045
PRIMER_LEFT_9_PENALTY=0.230329
PRIMER_RIGHT_9_PENALTY=2.051716
PRIMER_LEFT_9_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_9_SEQUENCE=CACGCTCTTGCAATGTTATG
PRIMER_LEFT_9=533,20
PRIMER_RIGHT_9=1451,20
PRIMER_LEFT_9_TM=60.230
PRIMER_RIGHT_9_TM=57.948
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=6.2000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=919
PRIMER_PAIR_10_PENALTY=2.318992
PRIMER_LEFT_10_PENALTY=0.230329
PRIMER_RIGHT_10_PENALTY=2.088662
PRIMER_LEFT_10_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_10_SEQUENCE=GGGTCTTAATGTTTTGGGTTCA
PRIMER_LEFT_10=533,20
PRIMER_RIGHT_10=1344,22
PRIMER_LEFT_10_TM=60.230
PRIMER_RIGHT_10_TM=60.089
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=812
PRIMER_PAIR_11_PENALTY=2.318992
PRIMER_LEFT_11_PENALTY=0.230329
PRIMER_RIGHT_11_PENALTY=2.088662
PRIMER_LEFT_11_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_11_SEQUENCE=TGGGTCTTAATGTTTTGGGTTC
PRIMER_LEFT_11=533,20
PRIMER_RIGHT_11=1345,22
PRIMER_LEFT_11_TM=60.230
PRIMER_RIGHT_11_TM=60.089
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.6000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=813
PRIMER_PAIR_12_PENALTY=2.366346
PRIMER_LEFT_12_PENALTY=0.230329
PRIMER_RIGHT_12_PENALTY=2.136017
PRIMER_LEFT_12_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_12_SEQUENCE=TGTGGATAGTCTTCCTTTGGGT
PRIMER_LEFT_12=533,20
PRIMER_RIGHT_12=1362,22
PRIMER_LEFT_12_TM=60.230
PRIMER_RIGHT_12_TM=59.864
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.455
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=830
PRIMER_PAIR_13_PENALTY=2.369103
PRIMER_LEFT_13_PENALTY=0.230329
PRIMER_RIGHT_13_PENALTY=2.138774
PRIMER_LEFT_13_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_13_SEQUENCE=CACGCTCTTGCAATGTTATGT
PRIMER_LEFT_13=533,20
PRIMER_RIGHT_13=1451,21
PRIMER_LEFT_13_TM=60.230
PRIMER_RIGHT_13_TM=58.861
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=5.6000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=919
PRIMER_PAIR_14_PENALTY=2.369103
PRIMER_LEFT_14_PENALTY=0.230329
PRIMER_RIGHT_14_PENALTY=2.138774
PRIMER_LEFT_14_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_14_SEQUENCE=ACACGCTCTTGCAATGTTATG
PRIMER_LEFT_14=533,20
PRIMER_RIGHT_14=1452,21
PRIMER_LEFT_14_TM=60.230
PRIMER_RIGHT_14_TM=58.861
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=6.2000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=920
PRIMER_PAIR_15_PENALTY=2.382423
PRIMER_LEFT_15_PENALTY=1.088471
PRIMER_RIGHT_15_PENALTY=1.293952
PRIMER_LEFT_15_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_15_SEQUENCE=TCAAGTTGCAAGACATGTGGA
PRIMER_LEFT_15=529,20
PRIMER_RIGHT_15=1377,21
PRIMER_LEFT_15_TM=58.912
PRIMER_RIGHT_15_TM=60.294
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=849
PRIMER_PAIR_16_PENALTY=2.423995
PRIMER_LEFT_16_PENALTY=0.230329
PRIMER_RIGHT_16_PENALTY=2.193666
PRIMER_LEFT_16_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_16_SEQUENCE=AACACGCTCTTGCAATGTTATG
PRIMER_LEFT_16=533,20
PRIMER_RIGHT_16=1453,22
PRIMER_LEFT_16_TM=60.230
PRIMER_RIGHT_16_TM=60.194
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=6.2000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=921
PRIMER_PAIR_17_PENALTY=2.483523
PRIMER_LEFT_17_PENALTY=0.230329
PRIMER_RIGHT_17_PENALTY=2.253194
PRIMER_LEFT_17_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_17_SEQUENCE=CTTTGGGTCTTAATGTTTTGGG
PRIMER_LEFT_17=533,20
PRIMER_RIGHT_17=1348,22
PRIMER_LEFT_17_TM=60.230
PRIMER_RIGHT_17_TM=59.747
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=40.909
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=10.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=816
PRIMER_PAIR_18_PENALTY=2.483523
PRIMER_LEFT_18_PENALTY=0.230329
PRIMER_RIGHT_18_PENALTY=2.253194
PRIMER_LEFT_18_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_18_SEQUENCE=CCTTTGGGTCTTAATGTTTTGG
PRIMER_LEFT_18=533,20
PRIMER_RIGHT_18=1349,22
PRIMER_LEFT_18_TM=60.230
PRIMER_RIGHT_18_TM=59.747
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.6000
PRIMER_RIGHT_18_END_STABILITY=8.8000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=817
PRIMER_PAIR_19_PENALTY=2.642364
PRIMER_LEFT_19_PENALTY=0.230329
PRIMER_RIGHT_19_PENALTY=2.412035
PRIMER_LEFT_19_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_19_SEQUENCE=GTTTTGGGTTCATATTTGAGGG
PRIMER_LEFT_19=533,20
PRIMER_RIGHT_19=1334,22
PRIMER_LEFT_19_TM=60.230
PRIMER_RIGHT_19_TM=59.588
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=802
PRIMER_PAIR_20_PENALTY=2.768781
PRIMER_LEFT_20_PENALTY=0.230329
PRIMER_RIGHT_20_PENALTY=2.538452
PRIMER_LEFT_20_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_20_SEQUENCE=TTGGGTCTTAATGTTTTGGG
PRIMER_LEFT_20=533,20
PRIMER_RIGHT_20=1346,20
PRIMER_LEFT_20_TM=60.230
PRIMER_RIGHT_20_TM=57.462
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.6000
PRIMER_RIGHT_20_END_STABILITY=10.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=814
PRIMER_PAIR_21_PENALTY=2.828498
PRIMER_LEFT_21_PENALTY=0.230329
PRIMER_RIGHT_21_PENALTY=2.598168
PRIMER_LEFT_21_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_21_SEQUENCE=TTTGGGTTCATATTTGAGGG
PRIMER_LEFT_21=533,20
PRIMER_RIGHT_21=1332,20
PRIMER_LEFT_21_TM=60.230
PRIMER_RIGHT_21_TM=57.402
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=800
PRIMER_PAIR_22_PENALTY=2.840247
PRIMER_LEFT_22_PENALTY=2.456937
PRIMER_RIGHT_22_PENALTY=0.383310
PRIMER_LEFT_22_SEQUENCE=CCTTTAGGCATGTAAGCGAC
PRIMER_RIGHT_22_SEQUENCE=TTGGGTTCATATTTGAGGGG
PRIMER_LEFT_22=398,20
PRIMER_RIGHT_22=1331,20
PRIMER_LEFT_22_TM=57.543
PRIMER_RIGHT_22_TM=59.617
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=10.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=934
PRIMER_PAIR_23_PENALTY=2.842301
PRIMER_LEFT_23_PENALTY=1.088471
PRIMER_RIGHT_23_PENALTY=1.753830
PRIMER_LEFT_23_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_23_SEQUENCE=CAAGTTGCAAGACATGTGGA
PRIMER_LEFT_23=530,20
PRIMER_RIGHT_23=1376,20
PRIMER_LEFT_23_TM=58.912
PRIMER_RIGHT_23_TM=58.246
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=847
PRIMER_PAIR_24_PENALTY=2.842301
PRIMER_LEFT_24_PENALTY=1.088471
PRIMER_RIGHT_24_PENALTY=1.753830
PRIMER_LEFT_24_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_24_SEQUENCE=CAAGTTGCAAGACATGTGGA
PRIMER_LEFT_24=529,20
PRIMER_RIGHT_24=1376,20
PRIMER_LEFT_24_TM=58.912
PRIMER_RIGHT_24_TM=58.246
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.3000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=848
PRIMER_PAIR_25_PENALTY=2.882011
PRIMER_LEFT_25_PENALTY=0.230329
PRIMER_RIGHT_25_PENALTY=2.651681
PRIMER_LEFT_25_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_25_SEQUENCE=ATGTGGATAGTCTTCCTTTGGG
PRIMER_LEFT_25=533,20
PRIMER_RIGHT_25=1363,22
PRIMER_LEFT_25_TM=60.230
PRIMER_RIGHT_25_TM=59.348
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=10.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=831
PRIMER_PAIR_26_PENALTY=2.892900
PRIMER_LEFT_26_PENALTY=1.139070
PRIMER_RIGHT_26_PENALTY=1.753830
PRIMER_LEFT_26_SEQUENCE=CTTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_26_SEQUENCE=CAAGTTGCAAGACATGTGGA
PRIMER_LEFT_26=529,21
PRIMER_RIGHT_26=1376,20
PRIMER_LEFT_26_TM=59.861
PRIMER_RIGHT_26_TM=58.246
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=848
PRIMER_PAIR_27_PENALTY=2.919345
PRIMER_LEFT_27_PENALTY=1.625393
PRIMER_RIGHT_27_PENALTY=1.293952
PRIMER_LEFT_27_SEQUENCE=TCCTTTAGGCATGTAAGCGAC
PRIMER_RIGHT_27_SEQUENCE=TCAAGTTGCAAGACATGTGGA
PRIMER_LEFT_27=397,21
PRIMER_RIGHT_27=1377,21
PRIMER_LEFT_27_TM=59.375
PRIMER_RIGHT_27_TM=60.294
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.6000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=981
PRIMER_PAIR_28_PENALTY=2.928327
PRIMER_LEFT_28_PENALTY=0.230329
PRIMER_RIGHT_28_PENALTY=2.697998
PRIMER_LEFT_28_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_28_SEQUENCE=CACGCTCTTGCAATGTTATGTC
PRIMER_LEFT_28=533,20
PRIMER_RIGHT_28=1451,22
PRIMER_LEFT_28_TM=60.230
PRIMER_RIGHT_28_TM=60.698
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.6000
PRIMER_RIGHT_28_END_STABILITY=6.3000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=919
PRIMER_PAIR_29_PENALTY=2.975859
PRIMER_LEFT_29_PENALTY=1.088471
PRIMER_RIGHT_29_PENALTY=1.887388
PRIMER_LEFT_29_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_29_SEQUENCE=TTTGGGTTCATATTTGAGGGG
PRIMER_LEFT_29=530,20
PRIMER_RIGHT_29=1332,21
PRIMER_LEFT_29_TM=58.912
PRIMER_RIGHT_29_TM=60.887
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=10.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=803
PRIMER_PAIR_30_PENALTY=2.975859
PRIMER_LEFT_30_PENALTY=1.088471
PRIMER_RIGHT_30_PENALTY=1.887388
PRIMER_LEFT_30_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_30_SEQUENCE=TTTGGGTTCATATTTGAGGGG
PRIMER_LEFT_30=529,20
PRIMER_RIGHT_30=1332,21
PRIMER_LEFT_30_TM=58.912
PRIMER_RIGHT_30_TM=60.887
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=10.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=804
PRIMER_PAIR_31_PENALTY=2.997617
PRIMER_LEFT_31_PENALTY=2.614307
PRIMER_RIGHT_31_PENALTY=0.383310
PRIMER_LEFT_31_SEQUENCE=TTCCTTTAGGCATGTAAGCGAC
PRIMER_RIGHT_31_SEQUENCE=TTGGGTTCATATTTGAGGGG
PRIMER_LEFT_31=396,22
PRIMER_RIGHT_31=1331,20
PRIMER_LEFT_31_TM=60.614
PRIMER_RIGHT_31_TM=59.617
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=10.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=936
PRIMER_PAIR_32_PENALTY=3.026458
PRIMER_LEFT_32_PENALTY=1.139070
PRIMER_RIGHT_32_PENALTY=1.887388
PRIMER_LEFT_32_SEQUENCE=CTTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_32_SEQUENCE=TTTGGGTTCATATTTGAGGGG
PRIMER_LEFT_32=529,21
PRIMER_RIGHT_32=1332,21
PRIMER_LEFT_32_TM=59.861
PRIMER_RIGHT_32_TM=60.887
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=10.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=804
PRIMER_PAIR_33_PENALTY=3.050126
PRIMER_LEFT_33_PENALTY=1.088471
PRIMER_RIGHT_33_PENALTY=1.961655
PRIMER_LEFT_33_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_33_SEQUENCE=TGTGGATAGTCTTCCTTTGGG
PRIMER_LEFT_33=530,20
PRIMER_RIGHT_33=1362,21
PRIMER_LEFT_33_TM=58.912
PRIMER_RIGHT_33_TM=59.038
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=833
PRIMER_PAIR_34_PENALTY=3.050126
PRIMER_LEFT_34_PENALTY=1.088471
PRIMER_RIGHT_34_PENALTY=1.961655
PRIMER_LEFT_34_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_34_SEQUENCE=TGTGGATAGTCTTCCTTTGGG
PRIMER_LEFT_34=529,20
PRIMER_RIGHT_34=1362,21
PRIMER_LEFT_34_TM=58.912
PRIMER_RIGHT_34_TM=59.038
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.3000
PRIMER_RIGHT_34_END_STABILITY=10.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=834
PRIMER_PAIR_35_PENALTY=3.100725
PRIMER_LEFT_35_PENALTY=1.139070
PRIMER_RIGHT_35_PENALTY=1.961655
PRIMER_LEFT_35_SEQUENCE=CTTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_35_SEQUENCE=TGTGGATAGTCTTCCTTTGGG
PRIMER_LEFT_35=529,21
PRIMER_RIGHT_35=1362,21
PRIMER_LEFT_35_TM=59.861
PRIMER_RIGHT_35_TM=59.038
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=10.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=834
PRIMER_PAIR_36_PENALTY=3.122706
PRIMER_LEFT_36_PENALTY=0.230329
PRIMER_RIGHT_36_PENALTY=2.892377
PRIMER_LEFT_36_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_36_SEQUENCE=TGGATAGTCTTCCTTTGGGTC
PRIMER_LEFT_36=533,20
PRIMER_RIGHT_36=1360,21
PRIMER_LEFT_36_TM=60.230
PRIMER_RIGHT_36_TM=58.108
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.6000
PRIMER_RIGHT_36_END_STABILITY=9.1000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=828
PRIMER_PAIR_37_PENALTY=3.129470
PRIMER_LEFT_37_PENALTY=0.230329
PRIMER_RIGHT_37_PENALTY=2.899141
PRIMER_LEFT_37_SEQUENCE=CTATTCGGCATTTTGAGCGT
PRIMER_RIGHT_37_SEQUENCE=TGGATAGTCTTCCTTTGGGTCT
PRIMER_LEFT_37=533,20
PRIMER_RIGHT_37=1360,22
PRIMER_LEFT_37_TM=60.230
PRIMER_RIGHT_37_TM=59.101
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=828
PRIMER_PAIR_38_PENALTY=3.140187
PRIMER_LEFT_38_PENALTY=1.088471
PRIMER_RIGHT_38_PENALTY=2.051716
PRIMER_LEFT_38_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_38_SEQUENCE=CACGCTCTTGCAATGTTATG
PRIMER_LEFT_38=530,20
PRIMER_RIGHT_38=1451,20
PRIMER_LEFT_38_TM=58.912
PRIMER_RIGHT_38_TM=57.948
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.2000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=922
PRIMER_PAIR_39_PENALTY=3.140187
PRIMER_LEFT_39_PENALTY=1.088471
PRIMER_RIGHT_39_PENALTY=2.051716
PRIMER_LEFT_39_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_39_SEQUENCE=CACGCTCTTGCAATGTTATG
PRIMER_LEFT_39=529,20
PRIMER_RIGHT_39=1451,20
PRIMER_LEFT_39_TM=58.912
PRIMER_RIGHT_39_TM=57.948
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=6.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=923
PRIMER_PAIR_40_PENALTY=3.177133
PRIMER_LEFT_40_PENALTY=1.088471
PRIMER_RIGHT_40_PENALTY=2.088662
PRIMER_LEFT_40_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_40_SEQUENCE=GGGTCTTAATGTTTTGGGTTCA
PRIMER_LEFT_40=530,20
PRIMER_RIGHT_40=1344,22
PRIMER_LEFT_40_TM=58.912
PRIMER_RIGHT_40_TM=60.089
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=815
PRIMER_PAIR_41_PENALTY=3.177133
PRIMER_LEFT_41_PENALTY=1.088471
PRIMER_RIGHT_41_PENALTY=2.088662
PRIMER_LEFT_41_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_41_SEQUENCE=TGGGTCTTAATGTTTTGGGTTC
PRIMER_LEFT_41=530,20
PRIMER_RIGHT_41=1345,22
PRIMER_LEFT_41_TM=58.912
PRIMER_RIGHT_41_TM=60.089
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=816
PRIMER_PAIR_42_PENALTY=3.177133
PRIMER_LEFT_42_PENALTY=1.088471
PRIMER_RIGHT_42_PENALTY=2.088662
PRIMER_LEFT_42_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_42_SEQUENCE=GGGTCTTAATGTTTTGGGTTCA
PRIMER_LEFT_42=529,20
PRIMER_RIGHT_42=1344,22
PRIMER_LEFT_42_TM=58.912
PRIMER_RIGHT_42_TM=60.089
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=816
PRIMER_PAIR_43_PENALTY=3.177133
PRIMER_LEFT_43_PENALTY=1.088471
PRIMER_RIGHT_43_PENALTY=2.088662
PRIMER_LEFT_43_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_43_SEQUENCE=TGGGTCTTAATGTTTTGGGTTC
PRIMER_LEFT_43=529,20
PRIMER_RIGHT_43=1345,22
PRIMER_LEFT_43_TM=58.912
PRIMER_RIGHT_43_TM=60.089
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=817
PRIMER_PAIR_44_PENALTY=3.190786
PRIMER_LEFT_44_PENALTY=1.139070
PRIMER_RIGHT_44_PENALTY=2.051716
PRIMER_LEFT_44_SEQUENCE=CTTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_44_SEQUENCE=CACGCTCTTGCAATGTTATG
PRIMER_LEFT_44=529,21
PRIMER_RIGHT_44=1451,20
PRIMER_LEFT_44_TM=59.861
PRIMER_RIGHT_44_TM=57.948
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=923
PRIMER_PAIR_45_PENALTY=3.224487
PRIMER_LEFT_45_PENALTY=1.088471
PRIMER_RIGHT_45_PENALTY=2.136017
PRIMER_LEFT_45_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_45_SEQUENCE=TGTGGATAGTCTTCCTTTGGGT
PRIMER_LEFT_45=530,20
PRIMER_RIGHT_45=1362,22
PRIMER_LEFT_45_TM=58.912
PRIMER_RIGHT_45_TM=59.864
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=833
PRIMER_PAIR_46_PENALTY=3.224487
PRIMER_LEFT_46_PENALTY=1.088471
PRIMER_RIGHT_46_PENALTY=2.136017
PRIMER_LEFT_46_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_46_SEQUENCE=TGTGGATAGTCTTCCTTTGGGT
PRIMER_LEFT_46=529,20
PRIMER_RIGHT_46=1362,22
PRIMER_LEFT_46_TM=58.912
PRIMER_RIGHT_46_TM=59.864
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=834
PRIMER_PAIR_47_PENALTY=3.227245
PRIMER_LEFT_47_PENALTY=1.088471
PRIMER_RIGHT_47_PENALTY=2.138774
PRIMER_LEFT_47_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_47_SEQUENCE=CACGCTCTTGCAATGTTATGT
PRIMER_LEFT_47=530,20
PRIMER_RIGHT_47=1451,21
PRIMER_LEFT_47_TM=58.912
PRIMER_RIGHT_47_TM=58.861
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=5.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=922
PRIMER_PAIR_48_PENALTY=3.227245
PRIMER_LEFT_48_PENALTY=1.088471
PRIMER_RIGHT_48_PENALTY=2.138774
PRIMER_LEFT_48_SEQUENCE=TTGCTATTCGGCATTTTGAG
PRIMER_RIGHT_48_SEQUENCE=ACACGCTCTTGCAATGTTATG
PRIMER_LEFT_48=530,20
PRIMER_RIGHT_48=1452,21
PRIMER_LEFT_48_TM=58.912
PRIMER_RIGHT_48_TM=58.861
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=923
PRIMER_PAIR_49_PENALTY=3.227245
PRIMER_LEFT_49_PENALTY=1.088471
PRIMER_RIGHT_49_PENALTY=2.138774
PRIMER_LEFT_49_SEQUENCE=CTTGCTATTCGGCATTTTGA
PRIMER_RIGHT_49_SEQUENCE=CACGCTCTTGCAATGTTATGT
PRIMER_LEFT_49=529,20
PRIMER_RIGHT_49=1451,21
PRIMER_LEFT_49_TM=58.912
PRIMER_RIGHT_49_TM=58.861
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=5.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.433_1_15879801_15879828_zu-0_mt-0_8991_109072_TRUE
SEQUENCE_TARGET=950,127
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_14103569_14104244_zu-0_hi-0_9259_382412_TRUE
SEQUENCE_TARGET=950,775
SEQUENCE_TEMPLATE=CAGTTTTGTGNGTGTCCTCCTCTTTCAAACAAACTTTTCTNCCACATATTGGGTTGATGCTTTAAACATGATTGTCCATCTATTTAATATTCTTCCCTCGAGCNTCAAAAACAANAANATTNTTTTTACTTTGCTNTTTAACTNAAACATTTTNNTTNTGATCATCTTCGNGNTTTTAGTTNNNTNTNCNNNNCATATCGTCTACNAAATTTGGCTAACAAGCTCTTACCTTGCTTCAATCAATGNNNNTATCTTTCTCGGTNNNNTATCCTTTGAACTATCGCGGTNGCTATTTCATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTAATCATCATCTCTCCTTACTTACNNACTAATCCNTCTTATTCTATCTATAATTTANGCTCTTTCTCANACAGCTACAATCCCTCATGTATATCNCTTTGTCTCACNATCACCAATCACTCCACCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGATTATACATCANCACTCCACATTGCCACATTTGATTCTTCAGCTCTCNTTATNNCTCTATCGCAGCATAATAGGTATTATTCGTTATAGTAGTTAAAACAGTTTACTTTTAACCCAAGTAACATATNCNTACTTTGATACTAGAAATGATTCTNNNNNNNNNNNNNNNNNNNNNNNNNNGATTCAAACTAANTAATGACTAGATTCGAATAGCTACTACGTACGNGATGGCACTAATGGTGAAAGCTGAAGGTGGAAATGGAGGGAAGCNATGGGATGATGGATTCGATTATGAGGGTGTAACAAAGATATATGTACGAGGAGGTCTTGAAGGCATNCAGTTCATCNAGTTNNACTATNTCAAAGATGGTAAAACTATTNCTGGACCAATNCATGGTGTCTCTGGTCGTGGTTTGACACAAACGGTACATANGTAANNNNNNNNNNNNNNNNNTNTATATCTNATTTNACAATCNTTTTTNTATATATATNNNNNNNNNNTTTTTGCAGTTTGAGATTAACNATTNG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_11677765_11677838_tsu-0_wu-0_1194617_15214_TRUE
SEQUENCE_TARGET=950,173
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5470486_5470571_oy-0_tsu-0_130616_6380_TRUE
SEQUENCE_TARGET=950,185
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17945492_17945567_ct-1_po-0_7035_18854_TRUE
SEQUENCE_TARGET=950,175
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNTGAATAGANTCTAAGTAGTCAATATTTTCTTANTAATGTTACNCGTCTCATNAANNTGGNTGTGGACTCTCTTTTAATTTTAGTNACTGCTCATTCNATNAAAANATAANAAACTNCAAGNTTATNNNNTNCTTAATNAAAATTTGAAANACNTTTGTATAAAGACAAATACTATTAGTANCANCATAACTCATTTNTTNCCNTATAAATAACAACTNATCTTNGNATTNTTNATCACTCAACAACAAACACAANAAANAATNATAAATTNNANTACTTCAAACATNTTTTTNCTTTNATNATGGCNATCAAAAANNTGATTTTTCTTCTATNGNTTNTATGNNTNNCAATGTCTNCAAATGCTCAATTACCTCAATTTCNGGCACCGTTTCNTTTTCCATTTNNANTNNNNNNNNNNNNNNNNNNNNTGNTATAACAAAATGTTTNTNATCCTTTATGAATATTNCANGGTGTATCGNAGAGNTTTCACAATCTATANTCANNGGAAAGTTTGGCAACATANNNNCNGNTTGTTNNAAAGCAATTTTNGAANCTNAAACCAANTGTATTCCNNAACTTCCATTCAANNNATTTTTTCCTCCTCTGCTAAANNAACAATGTTCAAAAANNNNTGGCNCAATTNNTCNTANNANAANNTAGAATTNAAAAANCAATAATNTAANNGTTGCNCTTTTTTCNANATTANAAAAATAANNTTAGGTTTACNNNTTGTATTCAAANTTTTCAAACATCTAATAATAGCATTATNNNAAAANAGNCNCNTTTTTGTNAACAGTCATNAAATAGTTCAAANTGNTAGCNATCTACATATTGNAAAAAGAAGCAATTCTTATGCAAATTCTANNAATATTGCTATTTTNATACTCANTAANTGGATATTCACATTNNAAANNNNTGAGCATCGTTNTNTAATATTCGTGGTNTGATTACAAGATCTATCTATGTAANTAAANTGAAAACGGANCAGNNATCCNCNNNNNNNNNNNNNNNNTNTNTGNNNNNTNNNTNTTTTTTTNNNANNAATNNNNNTTAATANTTNTTTTGCATNNTAGANTTACATATATCNAGACTTTTNANATCAAANTNAAACNGATAACAAATNGAANCGAANNTAACANATAATNNGTCTNATCCNTAATNTAATCTACATTTCCATCTTCTNTTTACACANTGANNTTTTAGATGATNAAAAANATAAAACNAACCAGAGTTTTTGTNGGTTCATACAANANACNNNNNNAAAANCAAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATTAAGAAATGGANAAAAAGAAGGAATANTAGNAANNNNNNGANTTCTGTAACGGCTAGTTCCAAAGTACCGTTNTTTTTTTNGTTTTAATTATTGTATTAAGGCACGCTCTGACACATTCACACTCANANANAGNGAGAAGAAAAGNNAAAAAAANNNTNNAAACTTTTGCTTNTACTCNATTCTCTNATTTAGGNNNNNNNNNNNNNTTTACACTATCTCGATTTGAGAAGNGAGAAGAGAAATCGCCATGAACATNTTCAAGAAGAAGACCACTCCCAAAGGTATNAAAAAGCTTNAAACTTTATCNCATTGTNTCTCTGAGATTNTGTTNNNNNNNNNNTTTTTTTCTTCATNTCTGGNGAAATCGCGAGGANTNGTTTNNNTATCTGTTGGGTAACANAAAANATGNNAGCTTTAAGTGTTCTTNGTGTTCAAAATTGAATCTTGTAGTGTGNGTNAGTGTGAATTTTTCTGGGTTCTNAGTTTCNANTNAANGNNNNNNTAGATAGATTCCTTAATTAGATGTGTAANTAAAGTNNNNNNNNNNNNNNNATTGNAAAATAGTNAGTTGNCNANANTTAGNTAACTTGTGNNNNNNNNAATTTTGCNTTTGATTCTNTTGAAAAANNTNTGANTTTTNNNTTCTAATNCTGCAGATGCTCTTCGTACTAGTAAGAGAGAAATGGCTGTTGCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14812051_14812418_mt-0_ws-0_94050_18361_TRUE
SEQUENCE_TARGET=950,467
SEQUENCE_TEMPLATE=AAATTGTTGGTAGATTCGAATNAACAACTTAACAGCCGTAACGGACGTTACANAATTGTTGGATGGNGTTAATTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANTCGATNNTTGTACCANNACATANNNNNNNNNNNNNCNTCAANNNNTTTTTNTTTTTNNNNCCNNNNNNNNNNNNNNNNCTCCTTCACAAAAANCCTAGGGAGCATNNNNNNNNNCGCCGGTTTGAGCTTGANATTNGANNGACATAGCTTTTTATTTTTGTTATTNAAGGACATAATTTCATCATCNTNTTTTNCAATNAACCTCTAATTCTTAAAAATGNTTTTAAAGGAAACGTNAACCANTGGTGGGATNTCTNGGAATTGATCNAATTTTAACTTNTTAATCTAGAGAGNAGTTTAATTAATGGGTGAATTAGGGAAGTCCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACNNNAGGAACGTCCATGGNTCGAACCTNAGTTATGTGGGTTTCAAAACAGACATTTACCACTAGACTAANNTAATTATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGTGAAAAGTNAGTTGGAGGGTTAAAGTATGCANCTTTANTGATGGAGCNGATGAATATTTTTCTTGTTTCAGACAGAAAGCAACCAAGTTATAGGAGATGAATTGGAAACTANGNAGAAAGANTNGAAAGAACTTAAGAANCGTTAAAGACAATGTAGGTAAAATAAGAGNAAGAGGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_13258073_13258276_can-0_po-0_187412_261761_TRUE
SEQUENCE_TARGET=950,303
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANCATTTCTGAGCTTGAGAACTTGNAGTTGAGGAGAATTCAGTAGCAAATTCGTTAGTAGCTTCCACCNCTCGTTTNNCCTAGTATTNTAGCTCCAAATGTACATCATTAATAGATCNNATAAAAGAAAANTATAATTTCAAAANANGCNCAAGTNGAAGTATCCAACGGTGGTCAANTTANNNNNNNACNAACGCCTGNNNNTCAAAAGTTTCTNNAANTATTCATTAGCAGTGACGAATTCAAATTTGAAAAAANAANCTTTTNTCTGTAAATTNTCCATCCTTAACGGTCAAATTTAGTCGGGCAGCAGACAAACTCTCAAGTCCGATCAGNAAACGAAACGAGAACAAAAGAAAAGAGATTGGATTTTGGTGACGGCGTTTCCTTCGCNGTCGTGTTCACCATGTTCAAGAGAGACGACTACATTCGAANTAATNATGACCCCTTNTNTTCNAAGTGGCAGGNACTCTCGCATTTCAAGAAATTTANAGNTTCTGGGTTTAGGGTTTTGNTCCATCTTTCATTCCGTTGAAAACCCTTTCGAGATCAACAGAATTTCGAATGAATTCTCTTGGAAATATCNGNAATTTTTATTTAAAGATCTAANTTTTAACGCCTACCACACTTTTCTTCAACGTTTCTTTGTTGGATCTCTGGAGATTCTGAGTAATTNCATTGATGATTCTTGGGTTTCTTTCAGGGTTTCNCCCGATNTATGTTTCTGCGTAAACCCATTTCAGAAACTGCAGAATNCGCAAGACTTTTGCGGACTACTCCTTAATTTCTCGAGATTTAGGTNNNNNNCCAAAGGTCACATTTTTAATCTCNCTTCATCACATTGTAACTTGTATNTTTGNTNGATAACTAGGTGGCNTAGTCTCATGTTTGTCATCAATATAAGCTCTTTATNTNNNNTNATGAAGTTGNAAAACACACATTTTTAAATGTCAAACTTATTANACTTGAAAACAGATTCTGNNAGGAGCCAACGANAAGGAAAATTTCAGAGAAGGNAAAGATCNTGTTGGTAGAAACAGAGTCCAAGGAGCCTTCCAGGGTCTGTATGAGCTCAGTCATNACCATGGCAGAAAAGATGATGTTCTTGTGGCTAATCTTGGTCAACCTGAAAGCATTAGATCNNGGCTCCGGTCTTATAGTAGATCTTTTGCTCATCATGACTTGCTTAAGCAGGGTTTNTCTCAAACNATTCTTCCTACTACTCAGAACAAAAGTGACAANCNAACCGAAGAGAAGAAGTCTGATTCTGAAGAAGAGAGAGAGGTCAGTTCTGATGCTGCTGANAAGGAGAGTANTTCNCTTCCATCTATACTTAGACTCAGTCGGTCTAGACCTCAACCGGTTTCAGAGAAACATGATGACATAGTTGATGAGTCTGATTCTGCTTCTGCTTGTGGAGTCTTACTAGAAGATGGTACTACTTGCACTACAACTCCAGTCAAAGGNAGAAAGAGATGCACAGAGCATAAAGGGAAGAGACTTTCNCNTGTTTCCCCCGGNANNCACATACCGTGTGAAGTTCCTACCGTAAGAGAATGTGAAGAAANTGAGAATATATGTGGAGTGATTTTACCTGACATGNTACGTTGCAGAAGTAAACCTGTTTCAAGGAGAAAACGATGCGAGGATCACAAGGGAATGAGAGTNAACGCCTTCTTNTTTCTGCTGAACCCAACAGAACGTGNTAAAGCCGTCAANGAGGANAAATCNAAANCNGAGACNAGTACAGGCATGAACCAAGAGGGTTCNGGTCTTTTATGTGAAGCTACAACAAAGAATGGNTTGNCTTGTACAAGAAGTGCTCCTGAGGGNAGCANAAGATGTTGGCAGCACAAAGATAAAACNTTGAACCACGGATCATCNGAAAATGTNCAGTCAGCAACTGCAAGTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.907850
PRIMER_LEFT_0_PENALTY=0.221594
PRIMER_RIGHT_0_PENALTY=0.686256
PRIMER_LEFT_0_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_0_SEQUENCE=CTCCACAAGCAGAAGCAGAA
PRIMER_LEFT_0=812,20
PRIMER_RIGHT_0=1710,20
PRIMER_LEFT_0_TM=59.778
PRIMER_RIGHT_0_TM=59.314
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=899
PRIMER_PAIR_1_PENALTY=1.280506
PRIMER_LEFT_1_PENALTY=0.594250
PRIMER_RIGHT_1_PENALTY=0.686256
PRIMER_LEFT_1_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_1_SEQUENCE=CTCCACAAGCAGAAGCAGAA
PRIMER_LEFT_1=814,20
PRIMER_RIGHT_1=1710,20
PRIMER_LEFT_1_TM=59.406
PRIMER_RIGHT_1_TM=59.314
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=897
PRIMER_PAIR_2_PENALTY=1.280506
PRIMER_LEFT_2_PENALTY=0.594250
PRIMER_RIGHT_2_PENALTY=0.686256
PRIMER_LEFT_2_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_2_SEQUENCE=CTCCACAAGCAGAAGCAGAA
PRIMER_LEFT_2=813,20
PRIMER_RIGHT_2=1710,20
PRIMER_LEFT_2_TM=59.406
PRIMER_RIGHT_2_TM=59.314
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=898
PRIMER_PAIR_3_PENALTY=1.284463
PRIMER_LEFT_3_PENALTY=0.154878
PRIMER_RIGHT_3_PENALTY=1.129584
PRIMER_LEFT_3_SEQUENCE=AAATTTAGTCGGGCAGCAGA
PRIMER_RIGHT_3_SEQUENCE=GGCTCCTTGGACTCTGTTTCT
PRIMER_LEFT_3=585,20
PRIMER_RIGHT_3=1343,21
PRIMER_LEFT_3_TM=59.845
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=759
PRIMER_PAIR_4_PENALTY=1.339243
PRIMER_LEFT_4_PENALTY=0.209659
PRIMER_RIGHT_4_PENALTY=1.129584
PRIMER_LEFT_4_SEQUENCE=AGATTGGATTTTGGTGACGG
PRIMER_RIGHT_4_SEQUENCE=GGCTCCTTGGACTCTGTTTCT
PRIMER_LEFT_4=652,20
PRIMER_RIGHT_4=1343,21
PRIMER_LEFT_4_TM=59.790
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=692
PRIMER_PAIR_5_PENALTY=1.339243
PRIMER_LEFT_5_PENALTY=0.209659
PRIMER_RIGHT_5_PENALTY=1.129584
PRIMER_LEFT_5_SEQUENCE=AGATTGGATTTTGGTGACGG
PRIMER_RIGHT_5_SEQUENCE=AGGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_5=652,20
PRIMER_RIGHT_5=1344,21
PRIMER_LEFT_5_TM=59.790
PRIMER_RIGHT_5_TM=59.870
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=52.381
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=693
PRIMER_PAIR_6_PENALTY=1.339243
PRIMER_LEFT_6_PENALTY=0.209659
PRIMER_RIGHT_6_PENALTY=1.129584
PRIMER_LEFT_6_SEQUENCE=AGATTGGATTTTGGTGACGG
PRIMER_RIGHT_6_SEQUENCE=GAAGGCTCCTTGGACTCTGTT
PRIMER_LEFT_6=652,20
PRIMER_RIGHT_6=1346,21
PRIMER_LEFT_6_TM=59.790
PRIMER_RIGHT_6_TM=59.870
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=695
PRIMER_PAIR_7_PENALTY=1.341364
PRIMER_LEFT_7_PENALTY=0.209659
PRIMER_RIGHT_7_PENALTY=1.131705
PRIMER_LEFT_7_SEQUENCE=AGATTGGATTTTGGTGACGG
PRIMER_RIGHT_7_SEQUENCE=GGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_7=652,20
PRIMER_RIGHT_7=1343,20
PRIMER_LEFT_7_TM=59.790
PRIMER_RIGHT_7_TM=58.868
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=692
PRIMER_PAIR_8_PENALTY=1.351178
PRIMER_LEFT_8_PENALTY=0.221594
PRIMER_RIGHT_8_PENALTY=1.129584
PRIMER_LEFT_8_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_8_SEQUENCE=GGCTCCTTGGACTCTGTTTCT
PRIMER_LEFT_8=812,20
PRIMER_RIGHT_8=1343,21
PRIMER_LEFT_8_TM=59.778
PRIMER_RIGHT_8_TM=59.870
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=532
PRIMER_PAIR_9_PENALTY=1.351178
PRIMER_LEFT_9_PENALTY=0.221594
PRIMER_RIGHT_9_PENALTY=1.129584
PRIMER_LEFT_9_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_9_SEQUENCE=AGGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_9=812,20
PRIMER_RIGHT_9=1344,21
PRIMER_LEFT_9_TM=59.778
PRIMER_RIGHT_9_TM=59.870
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=52.381
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=533
PRIMER_PAIR_10_PENALTY=1.351178
PRIMER_LEFT_10_PENALTY=0.221594
PRIMER_RIGHT_10_PENALTY=1.129584
PRIMER_LEFT_10_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_10_SEQUENCE=GAAGGCTCCTTGGACTCTGTT
PRIMER_LEFT_10=812,20
PRIMER_RIGHT_10=1346,21
PRIMER_LEFT_10_TM=59.778
PRIMER_RIGHT_10_TM=59.870
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=535
PRIMER_PAIR_11_PENALTY=1.353299
PRIMER_LEFT_11_PENALTY=0.221594
PRIMER_RIGHT_11_PENALTY=1.131705
PRIMER_LEFT_11_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_11_SEQUENCE=GGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_11=812,20
PRIMER_RIGHT_11=1343,20
PRIMER_LEFT_11_TM=59.778
PRIMER_RIGHT_11_TM=58.868
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=532
PRIMER_PAIR_12_PENALTY=1.407536
PRIMER_LEFT_12_PENALTY=0.221594
PRIMER_RIGHT_12_PENALTY=1.185942
PRIMER_LEFT_12_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_12_SEQUENCE=ACTCCACAAGCAGAAGCAGAA
PRIMER_LEFT_12=812,20
PRIMER_RIGHT_12=1711,21
PRIMER_LEFT_12_TM=59.778
PRIMER_RIGHT_12_TM=60.186
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=900
PRIMER_PAIR_13_PENALTY=1.407536
PRIMER_LEFT_13_PENALTY=0.221594
PRIMER_RIGHT_13_PENALTY=1.185942
PRIMER_LEFT_13_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_13_SEQUENCE=AAGACTCCACAAGCAGAAGCA
PRIMER_LEFT_13=812,20
PRIMER_RIGHT_13=1714,21
PRIMER_LEFT_13_TM=59.778
PRIMER_RIGHT_13_TM=60.186
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=903
PRIMER_PAIR_14_PENALTY=1.420716
PRIMER_LEFT_14_PENALTY=0.221594
PRIMER_RIGHT_14_PENALTY=1.199123
PRIMER_LEFT_14_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_14_SEQUENCE=AGACTCCACAAGCAGAAGCAG
PRIMER_LEFT_14=812,20
PRIMER_RIGHT_14=1713,21
PRIMER_LEFT_14_TM=59.778
PRIMER_RIGHT_14_TM=59.801
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=52.381
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=902
PRIMER_PAIR_15_PENALTY=1.469387
PRIMER_LEFT_15_PENALTY=0.221594
PRIMER_RIGHT_15_PENALTY=1.247793
PRIMER_LEFT_15_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_15_SEQUENCE=ACTCCACAAGCAGAAGCAGA
PRIMER_LEFT_15=812,20
PRIMER_RIGHT_15=1711,20
PRIMER_LEFT_15_TM=59.778
PRIMER_RIGHT_15_TM=58.752
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=900
PRIMER_PAIR_16_PENALTY=1.469387
PRIMER_LEFT_16_PENALTY=0.221594
PRIMER_RIGHT_16_PENALTY=1.247793
PRIMER_LEFT_16_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_16_SEQUENCE=GACTCCACAAGCAGAAGCAG
PRIMER_LEFT_16=812,20
PRIMER_RIGHT_16=1712,20
PRIMER_LEFT_16_TM=59.778
PRIMER_RIGHT_16_TM=58.752
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=901
PRIMER_PAIR_17_PENALTY=1.469387
PRIMER_LEFT_17_PENALTY=0.221594
PRIMER_RIGHT_17_PENALTY=1.247793
PRIMER_LEFT_17_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_17_SEQUENCE=AGACTCCACAAGCAGAAGCA
PRIMER_LEFT_17=812,20
PRIMER_RIGHT_17=1713,20
PRIMER_LEFT_17_TM=59.778
PRIMER_RIGHT_17_TM=58.752
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=902
PRIMER_PAIR_18_PENALTY=1.560641
PRIMER_LEFT_18_PENALTY=0.431057
PRIMER_RIGHT_18_PENALTY=1.129584
PRIMER_LEFT_18_SEQUENCE=GGTCAAATTTAGTCGGGCAG
PRIMER_RIGHT_18_SEQUENCE=GGCTCCTTGGACTCTGTTTCT
PRIMER_LEFT_18=581,20
PRIMER_RIGHT_18=1343,21
PRIMER_LEFT_18_TM=59.569
PRIMER_RIGHT_18_TM=59.870
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=763
PRIMER_PAIR_19_PENALTY=1.560641
PRIMER_LEFT_19_PENALTY=0.431057
PRIMER_RIGHT_19_PENALTY=1.129584
PRIMER_LEFT_19_SEQUENCE=GGTCAAATTTAGTCGGGCAG
PRIMER_RIGHT_19_SEQUENCE=AGGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_19=581,20
PRIMER_RIGHT_19=1344,21
PRIMER_LEFT_19_TM=59.569
PRIMER_RIGHT_19_TM=59.870
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=764
PRIMER_PAIR_20_PENALTY=1.560641
PRIMER_LEFT_20_PENALTY=0.431057
PRIMER_RIGHT_20_PENALTY=1.129584
PRIMER_LEFT_20_SEQUENCE=GGTCAAATTTAGTCGGGCAG
PRIMER_RIGHT_20_SEQUENCE=GAAGGCTCCTTGGACTCTGTT
PRIMER_LEFT_20=581,20
PRIMER_RIGHT_20=1346,21
PRIMER_LEFT_20_TM=59.569
PRIMER_RIGHT_20_TM=59.870
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=766
PRIMER_PAIR_21_PENALTY=1.562762
PRIMER_LEFT_21_PENALTY=0.431057
PRIMER_RIGHT_21_PENALTY=1.131705
PRIMER_LEFT_21_SEQUENCE=GGTCAAATTTAGTCGGGCAG
PRIMER_RIGHT_21_SEQUENCE=GGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_21=581,20
PRIMER_RIGHT_21=1343,20
PRIMER_LEFT_21_TM=59.569
PRIMER_RIGHT_21_TM=58.868
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=763
PRIMER_PAIR_22_PENALTY=1.636958
PRIMER_LEFT_22_PENALTY=0.950702
PRIMER_RIGHT_22_PENALTY=0.686256
PRIMER_LEFT_22_SEQUENCE=TCCATCTTTCATTCCGTTGA
PRIMER_RIGHT_22_SEQUENCE=CTCCACAAGCAGAAGCAGAA
PRIMER_LEFT_22=806,20
PRIMER_RIGHT_22=1710,20
PRIMER_LEFT_22_TM=59.049
PRIMER_RIGHT_22_TM=59.314
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=905
PRIMER_PAIR_23_PENALTY=1.723834
PRIMER_LEFT_23_PENALTY=0.594250
PRIMER_RIGHT_23_PENALTY=1.129584
PRIMER_LEFT_23_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_23_SEQUENCE=GGCTCCTTGGACTCTGTTTCT
PRIMER_LEFT_23=814,20
PRIMER_RIGHT_23=1343,21
PRIMER_LEFT_23_TM=59.406
PRIMER_RIGHT_23_TM=59.870
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=530
PRIMER_PAIR_24_PENALTY=1.723834
PRIMER_LEFT_24_PENALTY=0.594250
PRIMER_RIGHT_24_PENALTY=1.129584
PRIMER_LEFT_24_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_24_SEQUENCE=AGGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_24=814,20
PRIMER_RIGHT_24=1344,21
PRIMER_LEFT_24_TM=59.406
PRIMER_RIGHT_24_TM=59.870
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=531
PRIMER_PAIR_25_PENALTY=1.723834
PRIMER_LEFT_25_PENALTY=0.594250
PRIMER_RIGHT_25_PENALTY=1.129584
PRIMER_LEFT_25_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_25_SEQUENCE=GAAGGCTCCTTGGACTCTGTT
PRIMER_LEFT_25=814,20
PRIMER_RIGHT_25=1346,21
PRIMER_LEFT_25_TM=59.406
PRIMER_RIGHT_25_TM=59.870
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=52.381
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.7000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=533
PRIMER_PAIR_26_PENALTY=1.723834
PRIMER_LEFT_26_PENALTY=0.594250
PRIMER_RIGHT_26_PENALTY=1.129584
PRIMER_LEFT_26_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_26_SEQUENCE=GGCTCCTTGGACTCTGTTTCT
PRIMER_LEFT_26=813,20
PRIMER_RIGHT_26=1343,21
PRIMER_LEFT_26_TM=59.406
PRIMER_RIGHT_26_TM=59.870
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.1000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=531
PRIMER_PAIR_27_PENALTY=1.723834
PRIMER_LEFT_27_PENALTY=0.594250
PRIMER_RIGHT_27_PENALTY=1.129584
PRIMER_LEFT_27_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_27_SEQUENCE=AGGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_27=813,20
PRIMER_RIGHT_27=1344,21
PRIMER_LEFT_27_TM=59.406
PRIMER_RIGHT_27_TM=59.870
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.1000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=532
PRIMER_PAIR_28_PENALTY=1.723834
PRIMER_LEFT_28_PENALTY=0.594250
PRIMER_RIGHT_28_PENALTY=1.129584
PRIMER_LEFT_28_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_28_SEQUENCE=GAAGGCTCCTTGGACTCTGTT
PRIMER_LEFT_28=813,20
PRIMER_RIGHT_28=1346,21
PRIMER_LEFT_28_TM=59.406
PRIMER_RIGHT_28_TM=59.870
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.1000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=534
PRIMER_PAIR_29_PENALTY=1.725955
PRIMER_LEFT_29_PENALTY=0.594250
PRIMER_RIGHT_29_PENALTY=1.131705
PRIMER_LEFT_29_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_29_SEQUENCE=GGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_29=814,20
PRIMER_RIGHT_29=1343,20
PRIMER_LEFT_29_TM=59.406
PRIMER_RIGHT_29_TM=58.868
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=530
PRIMER_PAIR_30_PENALTY=1.725955
PRIMER_LEFT_30_PENALTY=0.594250
PRIMER_RIGHT_30_PENALTY=1.131705
PRIMER_LEFT_30_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_30_SEQUENCE=GGCTCCTTGGACTCTGTTTC
PRIMER_LEFT_30=813,20
PRIMER_RIGHT_30=1343,20
PRIMER_LEFT_30_TM=59.406
PRIMER_RIGHT_30_TM=58.868
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.1000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=531
PRIMER_PAIR_31_PENALTY=1.776330
PRIMER_LEFT_31_PENALTY=0.154878
PRIMER_RIGHT_31_PENALTY=1.621451
PRIMER_LEFT_31_SEQUENCE=AAATTTAGTCGGGCAGCAGA
PRIMER_RIGHT_31_SEQUENCE=AAGGCTCCTTGGACTCTGTTT
PRIMER_LEFT_31=585,20
PRIMER_RIGHT_31=1345,21
PRIMER_LEFT_31_TM=59.845
PRIMER_RIGHT_31_TM=59.379
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=761
PRIMER_PAIR_32_PENALTY=1.780192
PRIMER_LEFT_32_PENALTY=0.594250
PRIMER_RIGHT_32_PENALTY=1.185942
PRIMER_LEFT_32_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_32_SEQUENCE=ACTCCACAAGCAGAAGCAGAA
PRIMER_LEFT_32=814,20
PRIMER_RIGHT_32=1711,21
PRIMER_LEFT_32_TM=59.406
PRIMER_RIGHT_32_TM=60.186
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=898
PRIMER_PAIR_33_PENALTY=1.780192
PRIMER_LEFT_33_PENALTY=0.594250
PRIMER_RIGHT_33_PENALTY=1.185942
PRIMER_LEFT_33_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_33_SEQUENCE=AAGACTCCACAAGCAGAAGCA
PRIMER_LEFT_33=814,20
PRIMER_RIGHT_33=1714,21
PRIMER_LEFT_33_TM=59.406
PRIMER_RIGHT_33_TM=60.186
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=901
PRIMER_PAIR_34_PENALTY=1.780192
PRIMER_LEFT_34_PENALTY=0.594250
PRIMER_RIGHT_34_PENALTY=1.185942
PRIMER_LEFT_34_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_34_SEQUENCE=ACTCCACAAGCAGAAGCAGAA
PRIMER_LEFT_34=813,20
PRIMER_RIGHT_34=1711,21
PRIMER_LEFT_34_TM=59.406
PRIMER_RIGHT_34_TM=60.186
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.1000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=899
PRIMER_PAIR_35_PENALTY=1.780192
PRIMER_LEFT_35_PENALTY=0.594250
PRIMER_RIGHT_35_PENALTY=1.185942
PRIMER_LEFT_35_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_35_SEQUENCE=AAGACTCCACAAGCAGAAGCA
PRIMER_LEFT_35=813,20
PRIMER_RIGHT_35=1714,21
PRIMER_LEFT_35_TM=59.406
PRIMER_RIGHT_35_TM=60.186
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.1000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=902
PRIMER_PAIR_36_PENALTY=1.793373
PRIMER_LEFT_36_PENALTY=0.594250
PRIMER_RIGHT_36_PENALTY=1.199123
PRIMER_LEFT_36_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_36_SEQUENCE=AGACTCCACAAGCAGAAGCAG
PRIMER_LEFT_36=814,20
PRIMER_RIGHT_36=1713,21
PRIMER_LEFT_36_TM=59.406
PRIMER_RIGHT_36_TM=59.801
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=900
PRIMER_PAIR_37_PENALTY=1.793373
PRIMER_LEFT_37_PENALTY=0.594250
PRIMER_RIGHT_37_PENALTY=1.199123
PRIMER_LEFT_37_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_37_SEQUENCE=AGACTCCACAAGCAGAAGCAG
PRIMER_LEFT_37=813,20
PRIMER_RIGHT_37=1713,21
PRIMER_LEFT_37_TM=59.406
PRIMER_RIGHT_37_TM=59.801
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.1000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=901
PRIMER_PAIR_38_PENALTY=1.831110
PRIMER_LEFT_38_PENALTY=0.209659
PRIMER_RIGHT_38_PENALTY=1.621451
PRIMER_LEFT_38_SEQUENCE=AGATTGGATTTTGGTGACGG
PRIMER_RIGHT_38_SEQUENCE=AAGGCTCCTTGGACTCTGTTT
PRIMER_LEFT_38=652,20
PRIMER_RIGHT_38=1345,21
PRIMER_LEFT_38_TM=59.790
PRIMER_RIGHT_38_TM=59.379
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=694
PRIMER_PAIR_39_PENALTY=1.842043
PRIMER_LEFT_39_PENALTY=0.594250
PRIMER_RIGHT_39_PENALTY=1.247793
PRIMER_LEFT_39_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_39_SEQUENCE=ACTCCACAAGCAGAAGCAGA
PRIMER_LEFT_39=814,20
PRIMER_RIGHT_39=1711,20
PRIMER_LEFT_39_TM=59.406
PRIMER_RIGHT_39_TM=58.752
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=898
PRIMER_PAIR_40_PENALTY=1.842043
PRIMER_LEFT_40_PENALTY=0.594250
PRIMER_RIGHT_40_PENALTY=1.247793
PRIMER_LEFT_40_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_40_SEQUENCE=GACTCCACAAGCAGAAGCAG
PRIMER_LEFT_40=814,20
PRIMER_RIGHT_40=1712,20
PRIMER_LEFT_40_TM=59.406
PRIMER_RIGHT_40_TM=58.752
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=899
PRIMER_PAIR_41_PENALTY=1.842043
PRIMER_LEFT_41_PENALTY=0.594250
PRIMER_RIGHT_41_PENALTY=1.247793
PRIMER_LEFT_41_SEQUENCE=TCATTCCGTTGAAAACCCTT
PRIMER_RIGHT_41_SEQUENCE=AGACTCCACAAGCAGAAGCA
PRIMER_LEFT_41=814,20
PRIMER_RIGHT_41=1713,20
PRIMER_LEFT_41_TM=59.406
PRIMER_RIGHT_41_TM=58.752
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=900
PRIMER_PAIR_42_PENALTY=1.842043
PRIMER_LEFT_42_PENALTY=0.594250
PRIMER_RIGHT_42_PENALTY=1.247793
PRIMER_LEFT_42_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_42_SEQUENCE=ACTCCACAAGCAGAAGCAGA
PRIMER_LEFT_42=813,20
PRIMER_RIGHT_42=1711,20
PRIMER_LEFT_42_TM=59.406
PRIMER_RIGHT_42_TM=58.752
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.1000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=899
PRIMER_PAIR_43_PENALTY=1.842043
PRIMER_LEFT_43_PENALTY=0.594250
PRIMER_RIGHT_43_PENALTY=1.247793
PRIMER_LEFT_43_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_43_SEQUENCE=GACTCCACAAGCAGAAGCAG
PRIMER_LEFT_43=813,20
PRIMER_RIGHT_43=1712,20
PRIMER_LEFT_43_TM=59.406
PRIMER_RIGHT_43_TM=58.752
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.1000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=900
PRIMER_PAIR_44_PENALTY=1.842043
PRIMER_LEFT_44_PENALTY=0.594250
PRIMER_RIGHT_44_PENALTY=1.247793
PRIMER_LEFT_44_SEQUENCE=TTCATTCCGTTGAAAACCCT
PRIMER_RIGHT_44_SEQUENCE=AGACTCCACAAGCAGAAGCA
PRIMER_LEFT_44=813,20
PRIMER_RIGHT_44=1713,20
PRIMER_LEFT_44_TM=59.406
PRIMER_RIGHT_44_TM=58.752
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.1000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=901
PRIMER_PAIR_45_PENALTY=1.843045
PRIMER_LEFT_45_PENALTY=0.221594
PRIMER_RIGHT_45_PENALTY=1.621451
PRIMER_LEFT_45_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_45_SEQUENCE=AAGGCTCCTTGGACTCTGTTT
PRIMER_LEFT_45=812,20
PRIMER_RIGHT_45=1345,21
PRIMER_LEFT_45_TM=59.778
PRIMER_RIGHT_45_TM=59.379
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=534
PRIMER_PAIR_46_PENALTY=1.856523
PRIMER_LEFT_46_PENALTY=0.154878
PRIMER_RIGHT_46_PENALTY=1.701644
PRIMER_LEFT_46_SEQUENCE=AAATTTAGTCGGGCAGCAGA
PRIMER_RIGHT_46_SEQUENCE=GAGCTCATACAGACCCTGGAA
PRIMER_LEFT_46=585,20
PRIMER_RIGHT_46=1364,21
PRIMER_LEFT_46_TM=59.845
PRIMER_RIGHT_46_TM=59.298
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=780
PRIMER_PAIR_47_PENALTY=1.911303
PRIMER_LEFT_47_PENALTY=0.209659
PRIMER_RIGHT_47_PENALTY=1.701644
PRIMER_LEFT_47_SEQUENCE=AGATTGGATTTTGGTGACGG
PRIMER_RIGHT_47_SEQUENCE=GAGCTCATACAGACCCTGGAA
PRIMER_LEFT_47=652,20
PRIMER_RIGHT_47=1364,21
PRIMER_LEFT_47_TM=59.790
PRIMER_RIGHT_47_TM=59.298
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=713
PRIMER_PAIR_48_PENALTY=1.923238
PRIMER_LEFT_48_PENALTY=0.221594
PRIMER_RIGHT_48_PENALTY=1.701644
PRIMER_LEFT_48_SEQUENCE=TTTCATTCCGTTGAAAACCC
PRIMER_RIGHT_48_SEQUENCE=GAGCTCATACAGACCCTGGAA
PRIMER_LEFT_48=812,20
PRIMER_RIGHT_48=1364,21
PRIMER_LEFT_48_TM=59.778
PRIMER_RIGHT_48_TM=59.298
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=553
PRIMER_PAIR_49_PENALTY=1.925921
PRIMER_LEFT_49_PENALTY=0.796337
PRIMER_RIGHT_49_PENALTY=1.129584
PRIMER_LEFT_49_SEQUENCE=ATTTAGTCGGGCAGCAGACA
PRIMER_RIGHT_49_SEQUENCE=GGCTCCTTGGACTCTGTTTCT
PRIMER_LEFT_49=587,20
PRIMER_RIGHT_49=1343,21
PRIMER_LEFT_49_TM=60.796
PRIMER_RIGHT_49_TM=59.870
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=757
=
SEQUENCE_ID=MAGIC.329_1_12666813_12667363_ws-0_ler-0_63164_83956_TRUE
SEQUENCE_TARGET=950,650
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_16485019_16485028_can-0_bur-0_13908_311_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=ATATTCGATTAGTTACATAATTCCTTCAACTTTTGATCCTTGTANACANATGTTACAATGTACAAAATATAATCTGTNGAAGTAAACCGNAACATNATNCAATGTGAGAACCGGATAAGATTATNNNTATTATTTATTTATANNNNNNNNNNNNNNNAAAGTAAATAAGTTGGTAAATATCGAGAATATAGGTTTTCAGACCAAACACATCACNTTGGTCCTACTTAGTACTTACCTGCCCCTCTCGACAAAATTTCTTNTGTACTTTCACATTTCTNTGTAATAAACTCGGTAGGTTTGCGAAAACCTNGCNGCCGGNAAAAAAAAAAATCAATGGAAGGAGGAGGNAGAGNACCAAATCAAACGATTCTCAGTGAAATAGAACATATGCCTGAAGCTCCACGTCAACGTATCTCTCATCACCGTCGAGCTCGCTCTGAAACCTTCTTCTCCGGCGAATCAATCGACGATCTCCTCTTATTCGATCCTTCCGANATCGATTTCTCTTCTCTAGACTTCCTCAACGCTCCACCACCACCACNACAATCACAACAACAACCNCAAGCTTCTCCCATGTCCGTTGATTCNGAAGAAACCTCATCNAACGGTGTTGTTCCTCCNAANNCTCTTCCTCCNAAACCCGAAGCTAGATTCGGTCGCCATGTTCGTAGCTTCTCGGTTGATTNCGATTTCTTCGATGATTTGGGTGTTACTGAGGAGAAGTNTNTAGCTACAAGTTCAGGAGAGAAGAAGAAAGGGNATCATCATCATAGCAGGAGTAATTCTATGGATGGAGAGATGAGTTCGGCGTCGTTTAATATCGAATCGATTTTAGCTTCTGTGAGTGGTAAAGATAGTGGGAAGAAGAATATGGGTATGGGTGGTGATAGACTTGCTGAGCTTGCTTTGCTTGATCCTAAAAGAGCTAAAAGGTATNTAATTTAGTGTTGNNNTTTACTTAAANNTTGTAACTTNTTTGNGATTTGATTGAGGTTTGAANNAGTGTTTTNNGNTGNNNTTTTGATAGGATTTTAGCGAATAGACAATCTGCGGCGAGGTCGAAAGAGAGGAAGATTAGGTATACTGGTGAGTTAGAGAGGAAGGTTCAGACACTTCAGAATGAAGCTACTACATTGTCTGCTCAAGTNACTATGTTACAGGTAATGTTTTNTATTACTTNGTCATNGTGTTTATGCAATTGGTGTGACTAGTGAAGGTTGGTGAGTANATTACNNNNNNTCTNNNNANCTNTNTNNNNNNNNNNNCTGATNTNNTNANTNNAGNTTNNNNNNNNGNACTNNNNNNNNTNNCTTGATTNGNNCNTGGCAGCTTNTANGCTTGTANTGNTGCTTAGTNTGTTTAAGATTGCTTCTTNGTGTNTAGAGAGGACATGTGTTTGCTACTAGNNNNNNNNATAGATGCAGNNTTATAAGATATNNTATTNTNATGTTAATTTAGCNNATNAATCAACATGTGAGATATGTCANTAGTGTAATGTATACANTTGCAAGAGTGTAANNTTTGTTTNCACTTTAGTGATAAAAGCAACATAGGAGTAAGATNAAATGATTCATGTNTCTTGCATCTCTAATCTGTGCTTTTTCTTATATTGTGTAGAGAGGAACATCAGAGCTGAACACTGAAAATAAACACCTCAAAATGCNGCTTCAAGCTTTAGAGCAACAAGCTGAACTTAGGGATGGTNAAGCCATTTTCTTNTTCATTGGNTCGTTCTCAATATGTANACCNAAGTCNNCNTTACGATATNTATTGTTCGGTGTAATTTTCAGCTTTGAATGAAGCNCTGCGGGATGAACTNAACCGACTTAAGGTGGTAGCTGGAGAAATTCCTCAGGGGAANGGAAATTCNTACAACCGTGCTCAATTCTCATCTCAGCAATCGGCAATGAATCAGTTTGGGAACAANANGAACCANCAGATGAGTACAAACGGGCAGCCATCGCTCCCAAGCTACATGGATTTCACCAAGAGAGGCTGAGTTCGTGTCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.823411
PRIMER_LEFT_0_PENALTY=0.777580
PRIMER_RIGHT_0_PENALTY=1.045831
PRIMER_LEFT_0_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_0_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_0=898,20
PRIMER_RIGHT_0=1850,21
PRIMER_LEFT_0_TM=59.222
PRIMER_RIGHT_0_TM=59.954
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=52.381
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.8000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=953
PRIMER_PAIR_1_PENALTY=2.053590
PRIMER_LEFT_1_PENALTY=0.777580
PRIMER_RIGHT_1_PENALTY=1.276010
PRIMER_LEFT_1_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_1_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_1=898,20
PRIMER_RIGHT_1=1891,20
PRIMER_LEFT_1_TM=59.222
PRIMER_RIGHT_1_TM=58.724
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=994
PRIMER_PAIR_2_PENALTY=2.161731
PRIMER_LEFT_2_PENALTY=0.095771
PRIMER_RIGHT_2_PENALTY=2.065960
PRIMER_LEFT_2_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_2_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_2=646,20
PRIMER_RIGHT_2=1220,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.066
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=575
PRIMER_PAIR_3_PENALTY=2.181722
PRIMER_LEFT_3_PENALTY=0.095771
PRIMER_RIGHT_3_PENALTY=2.085951
PRIMER_LEFT_3_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_3_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_3=646,20
PRIMER_RIGHT_3=1641,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=996
PRIMER_PAIR_4_PENALTY=2.383319
PRIMER_LEFT_4_PENALTY=0.317359
PRIMER_RIGHT_4_PENALTY=2.065960
PRIMER_LEFT_4_SEQUENCE=TTCTCCCATGTCCGTTGATT
PRIMER_RIGHT_4_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_4=566,20
PRIMER_RIGHT_4=1220,22
PRIMER_LEFT_4_TM=60.317
PRIMER_RIGHT_4_TM=60.066
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=655
PRIMER_PAIR_5_PENALTY=2.394411
PRIMER_LEFT_5_PENALTY=1.348580
PRIMER_RIGHT_5_PENALTY=1.045831
PRIMER_LEFT_5_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_5_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_5=892,20
PRIMER_RIGHT_5=1850,21
PRIMER_LEFT_5_TM=61.349
PRIMER_RIGHT_5_TM=59.954
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=52.381
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=959
PRIMER_PAIR_6_PENALTY=2.423693
PRIMER_LEFT_6_PENALTY=1.377862
PRIMER_RIGHT_6_PENALTY=1.045831
PRIMER_LEFT_6_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_6_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_6=899,21
PRIMER_RIGHT_6=1850,21
PRIMER_LEFT_6_TM=59.622
PRIMER_RIGHT_6_TM=59.954
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.5000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=952
PRIMER_PAIR_7_PENALTY=2.429008
PRIMER_LEFT_7_PENALTY=0.777580
PRIMER_RIGHT_7_PENALTY=1.651428
PRIMER_LEFT_7_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_7_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_7=898,20
PRIMER_RIGHT_7=1892,21
PRIMER_LEFT_7_TM=59.222
PRIMER_RIGHT_7_TM=60.651
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.8000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=995
PRIMER_PAIR_8_PENALTY=2.454367
PRIMER_LEFT_8_PENALTY=0.388407
PRIMER_RIGHT_8_PENALTY=2.065960
PRIMER_LEFT_8_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_8_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_8=788,20
PRIMER_RIGHT_8=1220,22
PRIMER_LEFT_8_TM=59.612
PRIMER_RIGHT_8_TM=60.066
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=10.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=433
PRIMER_PAIR_9_PENALTY=2.474358
PRIMER_LEFT_9_PENALTY=0.388407
PRIMER_RIGHT_9_PENALTY=2.085951
PRIMER_LEFT_9_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_9_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_9=788,20
PRIMER_RIGHT_9=1641,22
PRIMER_LEFT_9_TM=59.612
PRIMER_RIGHT_9_TM=59.914
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=10.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=854
PRIMER_PAIR_10_PENALTY=2.611197
PRIMER_LEFT_10_PENALTY=0.545237
PRIMER_RIGHT_10_PENALTY=2.065960
PRIMER_LEFT_10_SEQUENCE=AGACTTCCTCAACGCTCCAC
PRIMER_RIGHT_10_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_10=512,20
PRIMER_RIGHT_10=1220,22
PRIMER_LEFT_10_TM=59.455
PRIMER_RIGHT_10_TM=60.066
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=709
PRIMER_PAIR_11_PENALTY=2.624590
PRIMER_LEFT_11_PENALTY=1.348580
PRIMER_RIGHT_11_PENALTY=1.276010
PRIMER_LEFT_11_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_11_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_11=892,20
PRIMER_RIGHT_11=1891,20
PRIMER_LEFT_11_TM=61.349
PRIMER_RIGHT_11_TM=58.724
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=1000
PRIMER_PAIR_12_PENALTY=2.653872
PRIMER_LEFT_12_PENALTY=1.377862
PRIMER_RIGHT_12_PENALTY=1.276010
PRIMER_LEFT_12_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_12_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_12=899,21
PRIMER_RIGHT_12=1891,20
PRIMER_LEFT_12_TM=59.622
PRIMER_RIGHT_12_TM=58.724
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.5000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=993
PRIMER_PAIR_13_PENALTY=2.724911
PRIMER_LEFT_13_PENALTY=0.388407
PRIMER_RIGHT_13_PENALTY=2.336505
PRIMER_LEFT_13_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_13_SEQUENCE=CCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_13=788,20
PRIMER_RIGHT_13=1698,21
PRIMER_LEFT_13_TM=59.612
PRIMER_RIGHT_13_TM=58.663
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=10.2000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=911
PRIMER_PAIR_14_PENALTY=2.735536
PRIMER_LEFT_14_PENALTY=0.388407
PRIMER_RIGHT_14_PENALTY=2.347129
PRIMER_LEFT_14_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_14_SEQUENCE=CCATCCCTAAGTTCAGCTTGTT
PRIMER_LEFT_14=788,20
PRIMER_RIGHT_14=1702,22
PRIMER_LEFT_14_TM=59.612
PRIMER_RIGHT_14_TM=59.653
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=10.2000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=915
PRIMER_PAIR_15_PENALTY=2.812798
PRIMER_LEFT_15_PENALTY=0.388407
PRIMER_RIGHT_15_PENALTY=2.424392
PRIMER_LEFT_15_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_15_SEQUENCE=CCCTAAGTTCAGCTTGTTGCTC
PRIMER_LEFT_15=788,20
PRIMER_RIGHT_15=1698,22
PRIMER_LEFT_15_TM=59.612
PRIMER_RIGHT_15_TM=60.424
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=10.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=911
PRIMER_PAIR_16_PENALTY=2.812798
PRIMER_LEFT_16_PENALTY=0.388407
PRIMER_RIGHT_16_PENALTY=2.424392
PRIMER_LEFT_16_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_16_SEQUENCE=TCCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_16=788,20
PRIMER_RIGHT_16=1699,22
PRIMER_LEFT_16_TM=59.612
PRIMER_RIGHT_16_TM=60.424
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=10.2000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=912
PRIMER_PAIR_17_PENALTY=2.852117
PRIMER_LEFT_17_PENALTY=0.786156
PRIMER_RIGHT_17_PENALTY=2.065960
PRIMER_LEFT_17_SEQUENCE=CCATGTTCGTAGCTTCTCGG
PRIMER_RIGHT_17_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_17=659,20
PRIMER_RIGHT_17=1220,22
PRIMER_LEFT_17_TM=60.786
PRIMER_RIGHT_17_TM=60.066
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=562
PRIMER_PAIR_18_PENALTY=2.863531
PRIMER_LEFT_18_PENALTY=0.777580
PRIMER_RIGHT_18_PENALTY=2.085951
PRIMER_LEFT_18_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_18_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_18=898,20
PRIMER_RIGHT_18=1641,22
PRIMER_LEFT_18_TM=59.222
PRIMER_RIGHT_18_TM=59.914
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.8000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=744
PRIMER_PAIR_19_PENALTY=2.872108
PRIMER_LEFT_19_PENALTY=0.786156
PRIMER_RIGHT_19_PENALTY=2.085951
PRIMER_LEFT_19_SEQUENCE=CCATGTTCGTAGCTTCTCGG
PRIMER_RIGHT_19_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_19=659,20
PRIMER_RIGHT_19=1641,22
PRIMER_LEFT_19_TM=60.786
PRIMER_RIGHT_19_TM=59.914
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=983
PRIMER_PAIR_20_PENALTY=2.947204
PRIMER_LEFT_20_PENALTY=1.901373
PRIMER_RIGHT_20_PENALTY=1.045831
PRIMER_LEFT_20_SEQUENCE=GCTGAGCTTGCTTTGCTTGA
PRIMER_RIGHT_20_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_20=894,20
PRIMER_RIGHT_20=1850,21
PRIMER_LEFT_20_TM=61.901
PRIMER_RIGHT_20_TM=59.954
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=52.381
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=957
PRIMER_PAIR_21_PENALTY=2.993873
PRIMER_LEFT_21_PENALTY=0.927913
PRIMER_RIGHT_21_PENALTY=2.065960
PRIMER_LEFT_21_SEQUENCE=TGTTCGTAGCTTCTCGGTTG
PRIMER_RIGHT_21_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_21=662,20
PRIMER_RIGHT_21=1220,22
PRIMER_LEFT_21_TM=59.072
PRIMER_RIGHT_21_TM=60.066
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=559
PRIMER_PAIR_22_PENALTY=3.000386
PRIMER_LEFT_22_PENALTY=0.934426
PRIMER_RIGHT_22_PENALTY=2.065960
PRIMER_LEFT_22_SEQUENCE=CGCCATGTTCGTAGCTTCTC
PRIMER_RIGHT_22_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_22=657,20
PRIMER_RIGHT_22=1220,22
PRIMER_LEFT_22_TM=60.934
PRIMER_RIGHT_22_TM=60.066
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=564
PRIMER_PAIR_23_PENALTY=3.005507
PRIMER_LEFT_23_PENALTY=0.095771
PRIMER_RIGHT_23_PENALTY=2.909736
PRIMER_LEFT_23_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_23_SEQUENCE=TGTTCAGCTCTGATGTTCCTCT
PRIMER_LEFT_23=646,20
PRIMER_RIGHT_23=1638,22
PRIMER_LEFT_23_TM=60.096
PRIMER_RIGHT_23_TM=59.090
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=993
PRIMER_PAIR_24_PENALTY=3.013865
PRIMER_LEFT_24_PENALTY=0.927913
PRIMER_RIGHT_24_PENALTY=2.085951
PRIMER_LEFT_24_SEQUENCE=TGTTCGTAGCTTCTCGGTTG
PRIMER_RIGHT_24_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_24=662,20
PRIMER_RIGHT_24=1641,22
PRIMER_LEFT_24_TM=59.072
PRIMER_RIGHT_24_TM=59.914
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=980
PRIMER_PAIR_25_PENALTY=3.020378
PRIMER_LEFT_25_PENALTY=0.934426
PRIMER_RIGHT_25_PENALTY=2.085951
PRIMER_LEFT_25_SEQUENCE=CGCCATGTTCGTAGCTTCTC
PRIMER_RIGHT_25_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_25=657,20
PRIMER_RIGHT_25=1641,22
PRIMER_LEFT_25_TM=60.934
PRIMER_RIGHT_25_TM=59.914
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=985
PRIMER_PAIR_26_PENALTY=3.029290
PRIMER_LEFT_26_PENALTY=1.377862
PRIMER_RIGHT_26_PENALTY=1.651428
PRIMER_LEFT_26_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_26_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_26=899,21
PRIMER_RIGHT_26=1892,21
PRIMER_LEFT_26_TM=59.622
PRIMER_RIGHT_26_TM=60.651
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.5000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=994
PRIMER_PAIR_27_PENALTY=3.057135
PRIMER_LEFT_27_PENALTY=0.388407
PRIMER_RIGHT_27_PENALTY=2.668729
PRIMER_LEFT_27_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_27_SEQUENCE=CCATCCCTAAGTTCAGCTTGT
PRIMER_LEFT_27=788,20
PRIMER_RIGHT_27=1702,21
PRIMER_LEFT_27_TM=59.612
PRIMER_RIGHT_27_TM=58.331
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=10.2000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=915
PRIMER_PAIR_28_PENALTY=3.066601
PRIMER_LEFT_28_PENALTY=2.020770
PRIMER_RIGHT_28_PENALTY=1.045831
PRIMER_LEFT_28_SEQUENCE=GAGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_28_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_28=897,21
PRIMER_RIGHT_28=1850,21
PRIMER_LEFT_28_TM=61.021
PRIMER_RIGHT_28_TM=59.954
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.8000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=954
PRIMER_PAIR_29_PENALTY=3.068773
PRIMER_LEFT_29_PENALTY=1.002812
PRIMER_RIGHT_29_PENALTY=2.065960
PRIMER_LEFT_29_SEQUENCE=TAGATTCGGTCGCCATGTTC
PRIMER_RIGHT_29_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_29=647,20
PRIMER_RIGHT_29=1220,22
PRIMER_LEFT_29_TM=61.003
PRIMER_RIGHT_29_TM=60.066
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=574
PRIMER_PAIR_30_PENALTY=3.084497
PRIMER_LEFT_30_PENALTY=1.018537
PRIMER_RIGHT_30_PENALTY=2.065960
PRIMER_LEFT_30_SEQUENCE=AAGCTTCTCCCATGTCCGTT
PRIMER_RIGHT_30_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_30=562,20
PRIMER_RIGHT_30=1220,22
PRIMER_LEFT_30_TM=61.019
PRIMER_RIGHT_30_TM=60.066
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.9000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=659
PRIMER_PAIR_31_PENALTY=3.088764
PRIMER_LEFT_31_PENALTY=1.002812
PRIMER_RIGHT_31_PENALTY=2.085951
PRIMER_LEFT_31_SEQUENCE=TAGATTCGGTCGCCATGTTC
PRIMER_RIGHT_31_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_31=647,20
PRIMER_RIGHT_31=1641,22
PRIMER_LEFT_31_TM=61.003
PRIMER_RIGHT_31_TM=59.914
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=995
PRIMER_PAIR_32_PENALTY=3.114084
PRIMER_LEFT_32_PENALTY=0.777580
PRIMER_RIGHT_32_PENALTY=2.336505
PRIMER_LEFT_32_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_32_SEQUENCE=CCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_32=898,20
PRIMER_RIGHT_32=1698,21
PRIMER_LEFT_32_TM=59.222
PRIMER_RIGHT_32_TM=58.663
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.8000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=801
PRIMER_PAIR_33_PENALTY=3.124709
PRIMER_LEFT_33_PENALTY=0.777580
PRIMER_RIGHT_33_PENALTY=2.347129
PRIMER_LEFT_33_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_33_SEQUENCE=CCATCCCTAAGTTCAGCTTGTT
PRIMER_LEFT_33=898,20
PRIMER_RIGHT_33=1702,22
PRIMER_LEFT_33_TM=59.222
PRIMER_RIGHT_33_TM=59.653
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.8000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=805
PRIMER_PAIR_34_PENALTY=3.158797
PRIMER_LEFT_34_PENALTY=0.095771
PRIMER_RIGHT_34_PENALTY=3.063026
PRIMER_LEFT_34_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_34_SEQUENCE=GTGTTCAGCTCTGATGTTCCTCT
PRIMER_LEFT_34=646,20
PRIMER_RIGHT_34=1639,23
PRIMER_LEFT_34_TM=60.096
PRIMER_RIGHT_34_TM=59.937
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=47.826
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.6000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=994
PRIMER_PAIR_35_PENALTY=3.177383
PRIMER_LEFT_35_PENALTY=1.901373
PRIMER_RIGHT_35_PENALTY=1.276010
PRIMER_LEFT_35_SEQUENCE=GCTGAGCTTGCTTTGCTTGA
PRIMER_RIGHT_35_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_35=894,20
PRIMER_RIGHT_35=1891,20
PRIMER_LEFT_35_TM=61.901
PRIMER_RIGHT_35_TM=58.724
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=998
PRIMER_PAIR_36_PENALTY=3.201971
PRIMER_LEFT_36_PENALTY=0.777580
PRIMER_RIGHT_36_PENALTY=2.424392
PRIMER_LEFT_36_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_36_SEQUENCE=CCCTAAGTTCAGCTTGTTGCTC
PRIMER_LEFT_36=898,20
PRIMER_RIGHT_36=1698,22
PRIMER_LEFT_36_TM=59.222
PRIMER_RIGHT_36_TM=60.424
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.8000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=801
PRIMER_PAIR_37_PENALTY=3.201971
PRIMER_LEFT_37_PENALTY=0.777580
PRIMER_RIGHT_37_PENALTY=2.424392
PRIMER_LEFT_37_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_37_SEQUENCE=TCCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_37=898,20
PRIMER_RIGHT_37=1699,22
PRIMER_LEFT_37_TM=59.222
PRIMER_RIGHT_37_TM=60.424
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.8000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=802
PRIMER_PAIR_38_PENALTY=3.212235
PRIMER_LEFT_38_PENALTY=1.126283
PRIMER_RIGHT_38_PENALTY=2.085951
PRIMER_LEFT_38_SEQUENCE=GGGTGGTGATAGACTTGCTGA
PRIMER_RIGHT_38_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_38=878,21
PRIMER_RIGHT_38=1641,22
PRIMER_LEFT_38_TM=60.126
PRIMER_RIGHT_38_TM=59.914
PRIMER_LEFT_38_GC_PERCENT=52.381
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=764
PRIMER_PAIR_39_PENALTY=3.218388
PRIMER_LEFT_39_PENALTY=0.388407
PRIMER_RIGHT_39_PENALTY=2.829981
PRIMER_LEFT_39_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_39_SEQUENCE=ACCATCCCTAAGTTCAGCTTGT
PRIMER_LEFT_39=788,20
PRIMER_RIGHT_39=1703,22
PRIMER_LEFT_39_TM=59.612
PRIMER_RIGHT_39_TM=59.170
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=10.2000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=916
PRIMER_PAIR_40_PENALTY=3.248888
PRIMER_LEFT_40_PENALTY=1.182928
PRIMER_RIGHT_40_PENALTY=2.065960
PRIMER_LEFT_40_SEQUENCE=GCTCGCTCTGAAACCTTCTT
PRIMER_RIGHT_40_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_40=429,20
PRIMER_RIGHT_40=1220,22
PRIMER_LEFT_40_TM=58.817
PRIMER_RIGHT_40_TM=60.066
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=792
PRIMER_PAIR_41_PENALTY=3.279107
PRIMER_LEFT_41_PENALTY=1.213146
PRIMER_RIGHT_41_PENALTY=2.065960
PRIMER_LEFT_41_SEQUENCE=AGCTCGCTCTGAAACCTTCTT
PRIMER_RIGHT_41_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_41=428,21
PRIMER_RIGHT_41=1220,22
PRIMER_LEFT_41_TM=59.787
PRIMER_RIGHT_41_TM=60.066
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=793
PRIMER_PAIR_42_PENALTY=3.296780
PRIMER_LEFT_42_PENALTY=2.020770
PRIMER_RIGHT_42_PENALTY=1.276010
PRIMER_LEFT_42_SEQUENCE=GAGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_42_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_42=897,21
PRIMER_RIGHT_42=1891,20
PRIMER_LEFT_42_TM=61.021
PRIMER_RIGHT_42_TM=58.724
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.8000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=995
PRIMER_PAIR_43_PENALTY=3.298142
PRIMER_LEFT_43_PENALTY=0.388407
PRIMER_RIGHT_43_PENALTY=2.909736
PRIMER_LEFT_43_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_43_SEQUENCE=TGTTCAGCTCTGATGTTCCTCT
PRIMER_LEFT_43=788,20
PRIMER_RIGHT_43=1638,22
PRIMER_LEFT_43_TM=59.612
PRIMER_RIGHT_43_TM=59.090
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=10.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=851
PRIMER_PAIR_44_PENALTY=3.311084
PRIMER_LEFT_44_PENALTY=1.245123
PRIMER_RIGHT_44_PENALTY=2.065960
PRIMER_LEFT_44_SEQUENCE=GAAGCTCCACGTCAACGTATC
PRIMER_RIGHT_44_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_44=393,21
PRIMER_RIGHT_44=1220,22
PRIMER_LEFT_44_TM=59.755
PRIMER_RIGHT_44_TM=60.066
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=5.3000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=828
PRIMER_PAIR_45_PENALTY=3.348311
PRIMER_LEFT_45_PENALTY=1.282351
PRIMER_RIGHT_45_PENALTY=2.065960
PRIMER_LEFT_45_SEQUENCE=GAGCTCGCTCTGAAACCTTCT
PRIMER_RIGHT_45_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_45=427,21
PRIMER_RIGHT_45=1220,22
PRIMER_LEFT_45_TM=60.282
PRIMER_RIGHT_45_TM=60.066
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=794
PRIMER_PAIR_46_PENALTY=3.434531
PRIMER_LEFT_46_PENALTY=1.348580
PRIMER_RIGHT_46_PENALTY=2.085951
PRIMER_LEFT_46_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_46_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_46=892,20
PRIMER_RIGHT_46=1641,22
PRIMER_LEFT_46_TM=61.349
PRIMER_RIGHT_46_TM=59.914
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=750
PRIMER_PAIR_47_PENALTY=3.441588
PRIMER_LEFT_47_PENALTY=1.375627
PRIMER_RIGHT_47_PENALTY=2.065960
PRIMER_LEFT_47_SEQUENCE=CTAGACTTCCTCAACGCTCCA
PRIMER_RIGHT_47_SEQUENCE=CCAACCTTCACTAGTCACACCA
PRIMER_LEFT_47=510,21
PRIMER_RIGHT_47=1220,22
PRIMER_LEFT_47_TM=59.624
PRIMER_RIGHT_47_TM=60.066
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=711
PRIMER_PAIR_48_PENALTY=3.443213
PRIMER_LEFT_48_PENALTY=1.357262
PRIMER_RIGHT_48_PENALTY=2.085951
PRIMER_LEFT_48_SEQUENCE=TGGGTATGGGTGGTGATAGA
PRIMER_RIGHT_48_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_48=871,20
PRIMER_RIGHT_48=1641,22
PRIMER_LEFT_48_TM=58.643
PRIMER_RIGHT_48_TM=59.914
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=771
PRIMER_PAIR_49_PENALTY=3.446308
PRIMER_LEFT_49_PENALTY=0.777580
PRIMER_RIGHT_49_PENALTY=2.668729
PRIMER_LEFT_49_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_49_SEQUENCE=CCATCCCTAAGTTCAGCTTGT
PRIMER_LEFT_49=898,20
PRIMER_RIGHT_49=1702,21
PRIMER_LEFT_49_TM=59.222
PRIMER_RIGHT_49_TM=58.331
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.8000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=805
=
SEQUENCE_ID=MAGIC.105_4_9479486_9479583_po-0_bur-0_4034_9934_TRUE
SEQUENCE_TARGET=950,197
SEQUENCE_TEMPLATE=TAAAGTTATATTNACAANCGAAGCTGTGGTCCCTGCTACNAAGACAAGAAAGTTATCTGCTGAGCGATCAGAGGCTAGAAGGTTTGTTCATCATGACACCCCNTCATCATAATTACCANNCCTGTTGTTACANATGTTCTTCCTATTATGNATAAGTGTTTANNGTACTGCCATANTAACCGAGAAAATTTCNTCCAGCCACCTACTTCTTCAGAAACGCCAATTCTATCATTCTCACAGAGTCCAGGTGATCCAAGTTCCTTCACCTACTTCTTAGNCATTTTCTTTAAATTGCTCATGATGANATCTTATCAAAGCATACTTGGTTTGTTCTCATCNAAATTTGTANTTTGANTCTGTATGTATCAACGCAAAANANNTNTGNCCATGTTGTCTCCGTTTTATTGCCACTAACCAAAAANTNCATGTTTCNTGTGACAAGCCAATGGCGCTTGAGCAAGTAATGTCTGANCGGGATAGCGAGGATGAAGTNGATGANGATGTTGCAGATTTTGAAGATCGCCAGGTANTCCATGATTTNTTTCTNNGTTNNNNNNNNNNNNNNCAGNAAATGGTATANGATGTAACTTGCTAATGGCTTTTGAANCTTAAAAAAGCTGCAGATGCTTGATGACTTTGTGGATGTGAATAAAGATGAAAAGCAATTCATGCATCTTTGGAACTCGTTTGTAAGAAAACAAAGGTAACTACTTCTCTTACANTTGANCNNACACAANAAGACCTTNTGTCTTACATTCCATACCNGTNTAAATGATTNTGCTTATGGAACTTTGAGCTNNAANTATGNTTGNTGNNTNCAGGGTTATAGCAGATGGTCATATNTCNTGGGCATGTGAAGNATTNTCAAGATNTTACGAGAAAGAGTTGCACNGTTACTCATCACTCTTCTGGTAATANAAGTANACCAAACATATACAGACACATAACTACACTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAANNNCCAGGNGTTGGAGANTGTTTTTGATTAANCTATGGAACCATGGACTNGTCGACTCAGCCANCATCAACAACTGCAATACCATCCTCGAGAATTGCCNTAANANNNNNNNNNNNNNNNNNAACANNAANAGTGTGGATCNTCCCANTGACTCAAACACCAANAACAANAACANTGNGGATCATCNNANTGACATAAANAACAAGAACAATGTTGACAACAAGGACAATAACAGCAGAGNCAAGNAATTAANTAGGAAANNNNCCGGNTTNNATGATACNGNNNNATCGGATTGTAACTTATTCTTCTTTCTNAAAAAAATTGTTTNNNAGCAAACAAANNTTTNATNNGTTNGTGTATTCAACTGATTACANTTTTAGNNNNAANAANNNNNNNNNNNNNNNNNNNAAANACTGNAANNTAANNNAAGTTTNCTTANTTTTTCTTNNTGANTTGAGAAAAAGCTNNNNNNNNCCTCTNGTNAATANNNNNNNNNNNNNNNNNNNNNNNNNAACNNAAANATNTATATATNNAGTGCAAATAGATTGAAANCAAATNAAGAAATTAATTAAGACANAGTGATTAAGCTTAAAACCCCATTTNGACTTGTTCTTTCTCAATGAATCCCTCACANNNAGCAAGCTTCTTCGATTTTGCTTTGACACCACCAATCNGTGTTTTCNANTCTTTCAGGTTTNTCTCGATTNCAANCTAGATCGGAGTCAAGTNATANAATTGANTNACNTAANTANNNNNNTTCTCTCGTAAGNGTTGGGATTTAGCNGTAGATNGGAAATCGGANTTTACGTTTTTGTTANAANATTGATGNTTTAGGTNNTNNAACNNAGTTCTGGATTCNTTGCTTCTANTTGNTTCTCNAATTNTTTNATTNCGNAATNCACATNTTTGNTTCNAANGATCANANAATTTGNTTTAAAATTTGANAAANNNNNNNNNNNNNNNNNNNATNTTTCTTNNAGTAACTCGAACTTGTTGGAANTCTNNATANTCTGNGCTANNTAGANAAAGTCTTAACANTTTGNTCAACNNTGTTTGNTCNCTAAACTAGTTTGGGNTCTCTGTTTNNAAGTTTNNNNNNNNNNNT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.633757
PRIMER_LEFT_0_PENALTY=0.372058
PRIMER_RIGHT_0_PENALTY=1.261699
PRIMER_LEFT_0_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_0_SEQUENCE=GGTGTCAAAGCAAAATCGAAG
PRIMER_LEFT_0=661,20
PRIMER_RIGHT_0=1657,21
PRIMER_LEFT_0_TM=59.628
PRIMER_RIGHT_0_TM=59.738
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=997
PRIMER_PAIR_1_PENALTY=1.774311
PRIMER_LEFT_1_PENALTY=1.647773
PRIMER_RIGHT_1_PENALTY=0.126538
PRIMER_LEFT_1_SEQUENCE=GCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_1_SEQUENCE=TTGGTGGTGTCAAAGCAAAA
PRIMER_LEFT_1=670,20
PRIMER_RIGHT_1=1662,20
PRIMER_LEFT_1_TM=58.352
PRIMER_RIGHT_1_TM=60.127
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=993
PRIMER_PAIR_2_PENALTY=1.894127
PRIMER_LEFT_2_PENALTY=1.767588
PRIMER_RIGHT_2_PENALTY=0.126538
PRIMER_LEFT_2_SEQUENCE=GCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_2_SEQUENCE=TTGGTGGTGTCAAAGCAAAA
PRIMER_LEFT_2=670,21
PRIMER_RIGHT_2=1662,20
PRIMER_LEFT_2_TM=59.232
PRIMER_RIGHT_2_TM=60.127
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=993
PRIMER_PAIR_3_PENALTY=2.306691
PRIMER_LEFT_3_PENALTY=2.180153
PRIMER_RIGHT_3_PENALTY=0.126538
PRIMER_LEFT_3_SEQUENCE=TGCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_3_SEQUENCE=TTGGTGGTGTCAAAGCAAAA
PRIMER_LEFT_3=669,21
PRIMER_RIGHT_3=1662,20
PRIMER_LEFT_3_TM=61.180
PRIMER_RIGHT_3_TM=60.127
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=994
PRIMER_PAIR_4_PENALTY=2.324466
PRIMER_LEFT_4_PENALTY=0.372058
PRIMER_RIGHT_4_PENALTY=1.952408
PRIMER_LEFT_4_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_4_SEQUENCE=GGTGGTGTCAAAGCAAAATC
PRIMER_LEFT_4=661,20
PRIMER_RIGHT_4=1660,20
PRIMER_LEFT_4_TM=59.628
PRIMER_RIGHT_4_TM=58.048
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=1000
PRIMER_PAIR_5_PENALTY=2.743205
PRIMER_LEFT_5_PENALTY=1.767588
PRIMER_RIGHT_5_PENALTY=0.975617
PRIMER_LEFT_5_SEQUENCE=GCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_5_SEQUENCE=ATTGGTGGTGTCAAAGCAAA
PRIMER_LEFT_5=670,21
PRIMER_RIGHT_5=1663,20
PRIMER_LEFT_5_TM=59.232
PRIMER_RIGHT_5_TM=59.024
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=994
PRIMER_PAIR_6_PENALTY=2.909473
PRIMER_LEFT_6_PENALTY=1.647773
PRIMER_RIGHT_6_PENALTY=1.261699
PRIMER_LEFT_6_SEQUENCE=GCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_6_SEQUENCE=GGTGTCAAAGCAAAATCGAAG
PRIMER_LEFT_6=670,20
PRIMER_RIGHT_6=1657,21
PRIMER_LEFT_6_TM=58.352
PRIMER_RIGHT_6_TM=59.738
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=42.857
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=988
PRIMER_PAIR_7_PENALTY=3.029288
PRIMER_LEFT_7_PENALTY=1.767588
PRIMER_RIGHT_7_PENALTY=1.261699
PRIMER_LEFT_7_SEQUENCE=GCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_7_SEQUENCE=GGTGTCAAAGCAAAATCGAAG
PRIMER_LEFT_7=670,21
PRIMER_RIGHT_7=1657,21
PRIMER_LEFT_7_TM=59.232
PRIMER_RIGHT_7_TM=59.738
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=988
PRIMER_PAIR_8_PENALTY=3.201619
PRIMER_LEFT_8_PENALTY=0.372058
PRIMER_RIGHT_8_PENALTY=2.829561
PRIMER_LEFT_8_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_8_SEQUENCE=TGTGAGGGATTCATTGAGAAAG
PRIMER_LEFT_8=661,20
PRIMER_RIGHT_8=1624,22
PRIMER_LEFT_8_TM=59.628
PRIMER_RIGHT_8_TM=59.170
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=964
PRIMER_PAIR_9_PENALTY=3.441853
PRIMER_LEFT_9_PENALTY=2.180153
PRIMER_RIGHT_9_PENALTY=1.261699
PRIMER_LEFT_9_SEQUENCE=TGCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_9_SEQUENCE=GGTGTCAAAGCAAAATCGAAG
PRIMER_LEFT_9=669,21
PRIMER_RIGHT_9=1657,21
PRIMER_LEFT_9_TM=61.180
PRIMER_RIGHT_9_TM=59.738
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=989
PRIMER_PAIR_10_PENALTY=3.507784
PRIMER_LEFT_10_PENALTY=3.381246
PRIMER_RIGHT_10_PENALTY=0.126538
PRIMER_LEFT_10_SEQUENCE=ATGCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_10_SEQUENCE=TTGGTGGTGTCAAAGCAAAA
PRIMER_LEFT_10=668,22
PRIMER_RIGHT_10=1662,20
PRIMER_LEFT_10_TM=61.381
PRIMER_RIGHT_10_TM=60.127
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=995
PRIMER_PAIR_11_PENALTY=3.566785
PRIMER_LEFT_11_PENALTY=1.647773
PRIMER_RIGHT_11_PENALTY=1.919012
PRIMER_LEFT_11_SEQUENCE=GCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_11_SEQUENCE=TGGTGGTGTCAAAGCAAAATC
PRIMER_LEFT_11=670,20
PRIMER_RIGHT_11=1661,21
PRIMER_LEFT_11_TM=58.352
PRIMER_RIGHT_11_TM=60.919
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=992
PRIMER_PAIR_12_PENALTY=3.600181
PRIMER_LEFT_12_PENALTY=1.647773
PRIMER_RIGHT_12_PENALTY=1.952408
PRIMER_LEFT_12_SEQUENCE=GCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_12_SEQUENCE=GGTGGTGTCAAAGCAAAATC
PRIMER_LEFT_12=670,20
PRIMER_RIGHT_12=1660,20
PRIMER_LEFT_12_TM=58.352
PRIMER_RIGHT_12_TM=58.048
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=991
PRIMER_PAIR_13_PENALTY=3.686601
PRIMER_LEFT_13_PENALTY=1.767588
PRIMER_RIGHT_13_PENALTY=1.919012
PRIMER_LEFT_13_SEQUENCE=GCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_13_SEQUENCE=TGGTGGTGTCAAAGCAAAATC
PRIMER_LEFT_13=670,21
PRIMER_RIGHT_13=1661,21
PRIMER_LEFT_13_TM=59.232
PRIMER_RIGHT_13_TM=60.919
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=992
PRIMER_PAIR_14_PENALTY=3.686601
PRIMER_LEFT_14_PENALTY=1.767588
PRIMER_RIGHT_14_PENALTY=1.919012
PRIMER_LEFT_14_SEQUENCE=GCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_14_SEQUENCE=GATTGGTGGTGTCAAAGCAAA
PRIMER_LEFT_14=670,21
PRIMER_RIGHT_14=1664,21
PRIMER_LEFT_14_TM=59.232
PRIMER_RIGHT_14_TM=60.919
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=8.8000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=995
PRIMER_PAIR_15_PENALTY=3.719996
PRIMER_LEFT_15_PENALTY=1.767588
PRIMER_RIGHT_15_PENALTY=1.952408
PRIMER_LEFT_15_SEQUENCE=GCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_15_SEQUENCE=GGTGGTGTCAAAGCAAAATC
PRIMER_LEFT_15=670,21
PRIMER_RIGHT_15=1660,20
PRIMER_LEFT_15_TM=59.232
PRIMER_RIGHT_15_TM=58.048
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=991
PRIMER_PAIR_16_PENALTY=3.862391
PRIMER_LEFT_16_PENALTY=0.372058
PRIMER_RIGHT_16_PENALTY=3.490333
PRIMER_LEFT_16_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_16_SEQUENCE=GGTGTCAAAGCAAAATCGAAGA
PRIMER_LEFT_16=661,20
PRIMER_RIGHT_16=1657,22
PRIMER_LEFT_16_TM=59.628
PRIMER_RIGHT_16_TM=61.490
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=997
PRIMER_PAIR_17_PENALTY=4.055139
PRIMER_LEFT_17_PENALTY=3.928601
PRIMER_RIGHT_17_PENALTY=0.126538
PRIMER_LEFT_17_SEQUENCE=TGCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_17_SEQUENCE=TTGGTGGTGTCAAAGCAAAA
PRIMER_LEFT_17=669,22
PRIMER_RIGHT_17=1662,20
PRIMER_LEFT_17_TM=61.929
PRIMER_RIGHT_17_TM=60.127
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=994
PRIMER_PAIR_18_PENALTY=4.099165
PRIMER_LEFT_18_PENALTY=2.180153
PRIMER_RIGHT_18_PENALTY=1.919012
PRIMER_LEFT_18_SEQUENCE=TGCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_18_SEQUENCE=TGGTGGTGTCAAAGCAAAATC
PRIMER_LEFT_18=669,21
PRIMER_RIGHT_18=1661,21
PRIMER_LEFT_18_TM=61.180
PRIMER_RIGHT_18_TM=60.919
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=993
PRIMER_PAIR_19_PENALTY=4.132561
PRIMER_LEFT_19_PENALTY=2.180153
PRIMER_RIGHT_19_PENALTY=1.952408
PRIMER_LEFT_19_SEQUENCE=TGCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_19_SEQUENCE=GGTGGTGTCAAAGCAAAATC
PRIMER_LEFT_19=669,21
PRIMER_RIGHT_19=1660,20
PRIMER_LEFT_19_TM=61.180
PRIMER_RIGHT_19_TM=58.048
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=992
PRIMER_PAIR_20_PENALTY=4.331596
PRIMER_LEFT_20_PENALTY=0.372058
PRIMER_RIGHT_20_PENALTY=3.959538
PRIMER_LEFT_20_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_20_SEQUENCE=GTGTCAAAGCAAAATCGAAG
PRIMER_LEFT_20=661,20
PRIMER_RIGHT_20=1656,20
PRIMER_LEFT_20_TM=59.628
PRIMER_RIGHT_20_TM=56.040
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.7000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=996
PRIMER_PAIR_21_PENALTY=4.410558
PRIMER_LEFT_21_PENALTY=0.372058
PRIMER_RIGHT_21_PENALTY=4.038500
PRIMER_LEFT_21_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_21_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_21=661,20
PRIMER_RIGHT_21=1231,23
PRIMER_LEFT_21_TM=59.628
PRIMER_RIGHT_21_TM=58.961
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=43.478
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=571
PRIMER_PAIR_22_PENALTY=4.410564
PRIMER_LEFT_22_PENALTY=0.372058
PRIMER_RIGHT_22_PENALTY=4.038506
PRIMER_LEFT_22_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_22_SEQUENCE=TCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_22=661,20
PRIMER_RIGHT_22=1230,22
PRIMER_LEFT_22_TM=59.628
PRIMER_RIGHT_22_TM=57.961
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=570
PRIMER_PAIR_23_PENALTY=4.454282
PRIMER_LEFT_23_PENALTY=0.415781
PRIMER_RIGHT_23_PENALTY=4.038500
PRIMER_LEFT_23_SEQUENCE=GGCGCTTGAGCAAGTAATGT
PRIMER_RIGHT_23_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_23=447,20
PRIMER_RIGHT_23=1231,23
PRIMER_LEFT_23_TM=60.416
PRIMER_RIGHT_23_TM=58.961
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=785
PRIMER_PAIR_24_PENALTY=4.454288
PRIMER_LEFT_24_PENALTY=0.415781
PRIMER_RIGHT_24_PENALTY=4.038506
PRIMER_LEFT_24_SEQUENCE=GGCGCTTGAGCAAGTAATGT
PRIMER_RIGHT_24_SEQUENCE=TCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_24=447,20
PRIMER_RIGHT_24=1230,22
PRIMER_LEFT_24_TM=60.416
PRIMER_RIGHT_24_TM=57.961
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=784
PRIMER_PAIR_25_PENALTY=4.477334
PRIMER_LEFT_25_PENALTY=1.647773
PRIMER_RIGHT_25_PENALTY=2.829561
PRIMER_LEFT_25_SEQUENCE=GCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_25_SEQUENCE=TGTGAGGGATTCATTGAGAAAG
PRIMER_LEFT_25=670,20
PRIMER_RIGHT_25=1624,22
PRIMER_LEFT_25_TM=58.352
PRIMER_RIGHT_25_TM=59.170
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=955
PRIMER_PAIR_26_PENALTY=4.533311
PRIMER_LEFT_26_PENALTY=0.494811
PRIMER_RIGHT_26_PENALTY=4.038500
PRIMER_LEFT_26_SEQUENCE=TCCAGGTGATCCAAGTTCCT
PRIMER_RIGHT_26_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_26=242,20
PRIMER_RIGHT_26=1231,23
PRIMER_LEFT_26_TM=59.505
PRIMER_RIGHT_26_TM=58.961
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=43.478
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=990
PRIMER_PAIR_27_PENALTY=4.533317
PRIMER_LEFT_27_PENALTY=0.494811
PRIMER_RIGHT_27_PENALTY=4.038506
PRIMER_LEFT_27_SEQUENCE=TCCAGGTGATCCAAGTTCCT
PRIMER_RIGHT_27_SEQUENCE=TCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_27=242,20
PRIMER_RIGHT_27=1230,22
PRIMER_LEFT_27_TM=59.505
PRIMER_RIGHT_27_TM=57.961
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=989
PRIMER_PAIR_28_PENALTY=4.545338
PRIMER_LEFT_28_PENALTY=0.372058
PRIMER_RIGHT_28_PENALTY=4.173280
PRIMER_LEFT_28_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_28_SEQUENCE=CTGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_28=661,20
PRIMER_RIGHT_28=1229,23
PRIMER_LEFT_28_TM=59.628
PRIMER_RIGHT_28_TM=58.827
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=569
PRIMER_PAIR_29_PENALTY=4.559691
PRIMER_LEFT_29_PENALTY=0.372058
PRIMER_RIGHT_29_PENALTY=4.187633
PRIMER_LEFT_29_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_29_SEQUENCE=TGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_29=661,20
PRIMER_RIGHT_29=1228,22
PRIMER_LEFT_29_TM=59.628
PRIMER_RIGHT_29_TM=57.812
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=568
PRIMER_PAIR_30_PENALTY=4.589061
PRIMER_LEFT_30_PENALTY=0.415781
PRIMER_RIGHT_30_PENALTY=4.173280
PRIMER_LEFT_30_SEQUENCE=GGCGCTTGAGCAAGTAATGT
PRIMER_RIGHT_30_SEQUENCE=CTGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_30=447,20
PRIMER_RIGHT_30=1229,23
PRIMER_LEFT_30_TM=60.416
PRIMER_RIGHT_30_TM=58.827
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=43.478
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=783
PRIMER_PAIR_31_PENALTY=4.597149
PRIMER_LEFT_31_PENALTY=1.767588
PRIMER_RIGHT_31_PENALTY=2.829561
PRIMER_LEFT_31_SEQUENCE=GCATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_31_SEQUENCE=TGTGAGGGATTCATTGAGAAAG
PRIMER_LEFT_31=670,21
PRIMER_RIGHT_31=1624,22
PRIMER_LEFT_31_TM=59.232
PRIMER_RIGHT_31_TM=59.170
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=955
PRIMER_PAIR_32_PENALTY=4.603414
PRIMER_LEFT_32_PENALTY=0.415781
PRIMER_RIGHT_32_PENALTY=4.187633
PRIMER_LEFT_32_SEQUENCE=GGCGCTTGAGCAAGTAATGT
PRIMER_RIGHT_32_SEQUENCE=TGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_32=447,20
PRIMER_RIGHT_32=1228,22
PRIMER_LEFT_32_TM=60.416
PRIMER_RIGHT_32_TM=57.812
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=782
PRIMER_PAIR_33_PENALTY=4.618720
PRIMER_LEFT_33_PENALTY=0.372058
PRIMER_RIGHT_33_PENALTY=4.246662
PRIMER_LEFT_33_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_33_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTGT
PRIMER_LEFT_33=661,20
PRIMER_RIGHT_33=1231,24
PRIMER_LEFT_33_TM=59.628
PRIMER_RIGHT_33_TM=59.753
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=41.667
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=571
PRIMER_PAIR_34_PENALTY=4.642945
PRIMER_LEFT_34_PENALTY=3.381246
PRIMER_RIGHT_34_PENALTY=1.261699
PRIMER_LEFT_34_SEQUENCE=ATGCATCTTTGGAACTCGTTTG
PRIMER_RIGHT_34_SEQUENCE=GGTGTCAAAGCAAAATCGAAG
PRIMER_LEFT_34=668,22
PRIMER_RIGHT_34=1657,21
PRIMER_LEFT_34_TM=61.381
PRIMER_RIGHT_34_TM=59.738
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=8.7000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=990
PRIMER_PAIR_35_PENALTY=4.662444
PRIMER_LEFT_35_PENALTY=0.415781
PRIMER_RIGHT_35_PENALTY=4.246662
PRIMER_LEFT_35_SEQUENCE=GGCGCTTGAGCAAGTAATGT
PRIMER_RIGHT_35_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTGT
PRIMER_LEFT_35=447,20
PRIMER_RIGHT_35=1231,24
PRIMER_LEFT_35_TM=60.416
PRIMER_RIGHT_35_TM=59.753
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=41.667
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=785
PRIMER_PAIR_36_PENALTY=4.668091
PRIMER_LEFT_36_PENALTY=0.494811
PRIMER_RIGHT_36_PENALTY=4.173280
PRIMER_LEFT_36_SEQUENCE=TCCAGGTGATCCAAGTTCCT
PRIMER_RIGHT_36_SEQUENCE=CTGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_36=242,20
PRIMER_RIGHT_36=1229,23
PRIMER_LEFT_36_TM=59.505
PRIMER_RIGHT_36_TM=58.827
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=988
PRIMER_PAIR_37_PENALTY=4.679418
PRIMER_LEFT_37_PENALTY=0.640918
PRIMER_RIGHT_37_PENALTY=4.038500
PRIMER_LEFT_37_SEQUENCE=GTCCAGGTGATCCAAGTTCC
PRIMER_RIGHT_37_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_37=241,20
PRIMER_RIGHT_37=1231,23
PRIMER_LEFT_37_TM=59.359
PRIMER_RIGHT_37_TM=58.961
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=43.478
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=991
PRIMER_PAIR_38_PENALTY=4.679424
PRIMER_LEFT_38_PENALTY=0.640918
PRIMER_RIGHT_38_PENALTY=4.038506
PRIMER_LEFT_38_SEQUENCE=GTCCAGGTGATCCAAGTTCC
PRIMER_RIGHT_38_SEQUENCE=TCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_38=241,20
PRIMER_RIGHT_38=1230,22
PRIMER_LEFT_38_TM=59.359
PRIMER_RIGHT_38_TM=57.961
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=40.909
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=990
PRIMER_PAIR_39_PENALTY=4.682444
PRIMER_LEFT_39_PENALTY=0.494811
PRIMER_RIGHT_39_PENALTY=4.187633
PRIMER_LEFT_39_SEQUENCE=TCCAGGTGATCCAAGTTCCT
PRIMER_RIGHT_39_SEQUENCE=TGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_39=242,20
PRIMER_RIGHT_39=1228,22
PRIMER_LEFT_39_TM=59.505
PRIMER_RIGHT_39_TM=57.812
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=987
PRIMER_PAIR_40_PENALTY=4.741473
PRIMER_LEFT_40_PENALTY=0.494811
PRIMER_RIGHT_40_PENALTY=4.246662
PRIMER_LEFT_40_SEQUENCE=TCCAGGTGATCCAAGTTCCT
PRIMER_RIGHT_40_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTGT
PRIMER_LEFT_40=242,20
PRIMER_RIGHT_40=1231,24
PRIMER_LEFT_40_TM=59.505
PRIMER_RIGHT_40_TM=59.753
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=41.667
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=990
PRIMER_PAIR_41_PENALTY=4.753581
PRIMER_LEFT_41_PENALTY=0.372058
PRIMER_RIGHT_41_PENALTY=4.381523
PRIMER_LEFT_41_SEQUENCE=GCAATTCATGCATCTTTGGA
PRIMER_RIGHT_41_SEQUENCE=TGGTGTCAAAGCAAAATCGAAG
PRIMER_LEFT_41=661,20
PRIMER_RIGHT_41=1658,22
PRIMER_LEFT_41_TM=59.628
PRIMER_RIGHT_41_TM=62.382
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=998
PRIMER_PAIR_42_PENALTY=4.814198
PRIMER_LEFT_42_PENALTY=0.640918
PRIMER_RIGHT_42_PENALTY=4.173280
PRIMER_LEFT_42_SEQUENCE=GTCCAGGTGATCCAAGTTCC
PRIMER_RIGHT_42_SEQUENCE=CTGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_42=241,20
PRIMER_RIGHT_42=1229,23
PRIMER_LEFT_42_TM=59.359
PRIMER_RIGHT_42_TM=58.827
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=989
PRIMER_PAIR_43_PENALTY=4.828551
PRIMER_LEFT_43_PENALTY=0.640918
PRIMER_RIGHT_43_PENALTY=4.187633
PRIMER_LEFT_43_SEQUENCE=GTCCAGGTGATCCAAGTTCC
PRIMER_RIGHT_43_SEQUENCE=TGCTGTTATTGTCCTTGTTGTC
PRIMER_LEFT_43=241,20
PRIMER_RIGHT_43=1228,22
PRIMER_LEFT_43_TM=59.359
PRIMER_RIGHT_43_TM=57.812
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=988
PRIMER_PAIR_44_PENALTY=4.838889
PRIMER_LEFT_44_PENALTY=4.712351
PRIMER_RIGHT_44_PENALTY=0.126538
PRIMER_LEFT_44_SEQUENCE=CATCTTTGGAACTCGTTTGT
PRIMER_RIGHT_44_SEQUENCE=TTGGTGGTGTCAAAGCAAAA
PRIMER_LEFT_44=671,20
PRIMER_RIGHT_44=1662,20
PRIMER_LEFT_44_TM=55.288
PRIMER_RIGHT_44_TM=60.127
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=7.6000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=992
PRIMER_PAIR_45_PENALTY=4.860822
PRIMER_LEFT_45_PENALTY=0.822322
PRIMER_RIGHT_45_PENALTY=4.038500
PRIMER_LEFT_45_SEQUENCE=AAAGCTGCAGATGCTTGATG
PRIMER_RIGHT_45_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_45=613,20
PRIMER_RIGHT_45=1231,23
PRIMER_LEFT_45_TM=59.178
PRIMER_RIGHT_45_TM=58.961
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=43.478
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=619
PRIMER_PAIR_46_PENALTY=4.860828
PRIMER_LEFT_46_PENALTY=0.822322
PRIMER_RIGHT_46_PENALTY=4.038506
PRIMER_LEFT_46_SEQUENCE=AAAGCTGCAGATGCTTGATG
PRIMER_RIGHT_46_SEQUENCE=TCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_46=613,20
PRIMER_RIGHT_46=1230,22
PRIMER_LEFT_46_TM=59.178
PRIMER_RIGHT_46_TM=57.961
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=618
PRIMER_PAIR_47_PENALTY=4.887580
PRIMER_LEFT_47_PENALTY=0.640918
PRIMER_RIGHT_47_PENALTY=4.246662
PRIMER_LEFT_47_SEQUENCE=GTCCAGGTGATCCAAGTTCC
PRIMER_RIGHT_47_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTGT
PRIMER_LEFT_47=241,20
PRIMER_RIGHT_47=1231,24
PRIMER_LEFT_47_TM=59.359
PRIMER_RIGHT_47_TM=59.753
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=41.667
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=991
PRIMER_PAIR_48_PENALTY=4.891720
PRIMER_LEFT_48_PENALTY=0.853220
PRIMER_RIGHT_48_PENALTY=4.038500
PRIMER_LEFT_48_SEQUENCE=GCTGCAGATGCTTGATGACT
PRIMER_RIGHT_48_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_48=616,20
PRIMER_RIGHT_48=1231,23
PRIMER_LEFT_48_TM=59.147
PRIMER_RIGHT_48_TM=58.961
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=43.478
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=616
PRIMER_PAIR_49_PENALTY=4.891720
PRIMER_LEFT_49_PENALTY=0.853220
PRIMER_RIGHT_49_PENALTY=4.038500
PRIMER_LEFT_49_SEQUENCE=AGCTGCAGATGCTTGATGAC
PRIMER_RIGHT_49_SEQUENCE=CTCTGCTGTTATTGTCCTTGTTG
PRIMER_LEFT_49=615,20
PRIMER_RIGHT_49=1231,23
PRIMER_LEFT_49_TM=59.147
PRIMER_RIGHT_49_TM=58.961
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=43.478
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.3000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=617
=
SEQUENCE_ID=MAGIC.338_3_17385948_17386406_kn-0_hi-0_334171_40917_TRUE
SEQUENCE_TARGET=950,558
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGANACNAGTGCTTCTCTTGGAATCAGCTGGTNANGAATCTTGTTGCATCTTCAGAGTCCCTNANAGCTTCGCANAAATGAATCCTNAAGCTTATAAGCCCAAAGTTGTTTCCATTGGTCCTTACCATCACGGNAGAANACACCTNGAAATGATTCAACAGCANAAGCNTCGGTTTCTACACCNTTTCTTANNNANGGCAAGNGTNGATAGAGATGTNTTGTTTAANGCTGTAGTGGANTGGGAAGANGAGATAAGGANATCTTATTCTGANNNNNNNNNNNNNNNNNNNNNNNAACTAGTGTATATGATGATTCTTGATGGGTGCTTCATTCTCATGTTGTTACTTATCGTGTCAAGAAAGATAGAATTNTATGNGAGTGAGGATCCAATTNTCACNATCCCATGGATTCTNCCNTCTATTCAGAGTGATCTNCTCCTCTTGGAAAACCAGGTCCCTTTCTTNGTTCTTCAAACTTTATTTNACNAATCNNANATAGGTGNACCTGGTGATCTAAACAGGATGGCTTTTAGCTTCTTCAACCTCTCAATGGATAAACCAGANAGATATTGGGTCAAACACAGANACTTCAANGCAAAACATCTTCTTGACCTCATTCGTATGAGCNTCNTANCTATGGATGGATACNNNNNNNNNCAACTNACTAAGGGTAAATCTAGAAAGAAATCATCCTCCGGTNTTACATTGCTATTGTCGGCTACAAGACTTTCNCTCCAAGGAATAGATTTCAGTTTAAGGAGTGGTGCAGATTCGATNTTAGATATAAGNTTAAAGAAGAACAGGCTTCAAATACCAGTGTTAAGATTGGATGGGTTCATTANCTCAATCTTGCTCAATTGCGTCGCCTTTGAACAATTTTACGCAAAGAGTACNAACCACATAACAAGCTATGTGGTTTTCATGGGATNCCTTCTAAACGGCAAGGAAGATGCCACATTCTTNAGNCGTCNCCGGATCATAGAGAACTACNTTGGCAGNGAGAANGAGGTATCANAGTTCTTCAAAACTATATGTAAAGATGTNGTGTTTGACATACATGCAAGTTATTTACGGAATGTGTTTGTGGAAATTAACGAGAACACCTCAAAANGGTACTCANTATGTCGAANTTTTTTACNGTCACCAAGNAATTTTGTGGCATTNCNCTTTCGTCNANNTTATCGATTCTAAATACCATTCTTCTACCTTAGCAACAGCAACAGCAAAACCNCATGATGGAACGTCCTCNAGGCAAAGCNAANATGCTCGAGCTTATGTAGAAACAATGGAATTAAAGTCGTTTTTATCTTCTACAGAAANATCATTGTTCCCNNTTTTTTATCAGNNNNNNNNNTTGTGTTATTGCTATCTTTTTNGTTTTATGATNTTATCCTATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTNTACTTTCCTTTGATNANATANATTTGACTTNGCTTTGTCTTCTCGATTATCTTGCNCTATGAGAAGTCTTGAACTTGTCATGCNGCTGCCCTGACTTNGGGTGNACGGGATATAACTTCATGCTAATTTTCNGTGAAGTCCAAAANTTNATCGAAGTNGAGTNTCTATTTTTCTTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.352724
PRIMER_LEFT_0_PENALTY=1.197845
PRIMER_RIGHT_0_PENALTY=0.154878
PRIMER_LEFT_0_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_0_SEQUENCE=GCATCTTCCTTGCCGTTTAG
PRIMER_LEFT_0=873,20
PRIMER_RIGHT_0=1783,20
PRIMER_LEFT_0_TM=58.802
PRIMER_RIGHT_0_TM=59.845
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.1000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=911
PRIMER_PAIR_1_PENALTY=1.402444
PRIMER_LEFT_1_PENALTY=1.247566
PRIMER_RIGHT_1_PENALTY=0.154878
PRIMER_LEFT_1_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_1_SEQUENCE=GCATCTTCCTTGCCGTTTAG
PRIMER_LEFT_1=872,21
PRIMER_RIGHT_1=1783,20
PRIMER_LEFT_1_TM=60.248
PRIMER_RIGHT_1_TM=59.845
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=912
PRIMER_PAIR_2_PENALTY=1.512683
PRIMER_LEFT_2_PENALTY=1.197845
PRIMER_RIGHT_2_PENALTY=0.314838
PRIMER_LEFT_2_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_2_SEQUENCE=ATCGAATCTGCACCACTCCT
PRIMER_LEFT_2=873,20
PRIMER_RIGHT_2=1606,20
PRIMER_LEFT_2_TM=58.802
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=734
PRIMER_PAIR_3_PENALTY=1.562404
PRIMER_LEFT_3_PENALTY=1.247566
PRIMER_RIGHT_3_PENALTY=0.314838
PRIMER_LEFT_3_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_3_SEQUENCE=ATCGAATCTGCACCACTCCT
PRIMER_LEFT_3=872,21
PRIMER_RIGHT_3=1606,20
PRIMER_LEFT_3_TM=60.248
PRIMER_RIGHT_3_TM=59.685
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=735
PRIMER_PAIR_4_PENALTY=1.601266
PRIMER_LEFT_4_PENALTY=1.446387
PRIMER_RIGHT_4_PENALTY=0.154878
PRIMER_LEFT_4_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_4_SEQUENCE=GCATCTTCCTTGCCGTTTAG
PRIMER_LEFT_4=928,20
PRIMER_RIGHT_4=1783,20
PRIMER_LEFT_4_TM=58.554
PRIMER_RIGHT_4_TM=59.845
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=856
PRIMER_PAIR_5_PENALTY=1.761225
PRIMER_LEFT_5_PENALTY=1.446387
PRIMER_RIGHT_5_PENALTY=0.314838
PRIMER_LEFT_5_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_5_SEQUENCE=ATCGAATCTGCACCACTCCT
PRIMER_LEFT_5=928,20
PRIMER_RIGHT_5=1606,20
PRIMER_LEFT_5_TM=58.554
PRIMER_RIGHT_5_TM=59.685
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=679
PRIMER_PAIR_6_PENALTY=1.887867
PRIMER_LEFT_6_PENALTY=1.732988
PRIMER_RIGHT_6_PENALTY=0.154878
PRIMER_LEFT_6_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_6_SEQUENCE=GCATCTTCCTTGCCGTTTAG
PRIMER_LEFT_6=926,20
PRIMER_RIGHT_6=1783,20
PRIMER_LEFT_6_TM=58.267
PRIMER_RIGHT_6_TM=59.845
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=858
PRIMER_PAIR_7_PENALTY=1.999037
PRIMER_LEFT_7_PENALTY=1.197845
PRIMER_RIGHT_7_PENALTY=0.801192
PRIMER_LEFT_7_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_7_SEQUENCE=TCGAATCTGCACCACTCCTT
PRIMER_LEFT_7=873,20
PRIMER_RIGHT_7=1605,20
PRIMER_LEFT_7_TM=58.802
PRIMER_RIGHT_7_TM=60.801
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.1000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=733
PRIMER_PAIR_8_PENALTY=2.047826
PRIMER_LEFT_8_PENALTY=1.732988
PRIMER_RIGHT_8_PENALTY=0.314838
PRIMER_LEFT_8_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_8_SEQUENCE=ATCGAATCTGCACCACTCCT
PRIMER_LEFT_8=926,20
PRIMER_RIGHT_8=1606,20
PRIMER_LEFT_8_TM=58.267
PRIMER_RIGHT_8_TM=59.685
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=681
PRIMER_PAIR_9_PENALTY=2.048758
PRIMER_LEFT_9_PENALTY=1.247566
PRIMER_RIGHT_9_PENALTY=0.801192
PRIMER_LEFT_9_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_9_SEQUENCE=TCGAATCTGCACCACTCCTT
PRIMER_LEFT_9=872,21
PRIMER_RIGHT_9=1605,20
PRIMER_LEFT_9_TM=60.248
PRIMER_RIGHT_9_TM=60.801
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.1000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=734
PRIMER_PAIR_10_PENALTY=2.247579
PRIMER_LEFT_10_PENALTY=1.446387
PRIMER_RIGHT_10_PENALTY=0.801192
PRIMER_LEFT_10_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_10_SEQUENCE=TCGAATCTGCACCACTCCTT
PRIMER_LEFT_10=928,20
PRIMER_RIGHT_10=1605,20
PRIMER_LEFT_10_TM=58.554
PRIMER_RIGHT_10_TM=60.801
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=678
PRIMER_PAIR_11_PENALTY=2.534180
PRIMER_LEFT_11_PENALTY=1.732988
PRIMER_RIGHT_11_PENALTY=0.801192
PRIMER_LEFT_11_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_11_SEQUENCE=TCGAATCTGCACCACTCCTT
PRIMER_LEFT_11=926,20
PRIMER_RIGHT_11=1605,20
PRIMER_LEFT_11_TM=58.267
PRIMER_RIGHT_11_TM=60.801
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=680
PRIMER_PAIR_12_PENALTY=2.572630
PRIMER_LEFT_12_PENALTY=1.446387
PRIMER_RIGHT_12_PENALTY=1.126243
PRIMER_LEFT_12_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_12_SEQUENCE=TTTCCACAAACACATTCCGT
PRIMER_LEFT_12=928,20
PRIMER_RIGHT_12=1920,20
PRIMER_LEFT_12_TM=58.554
PRIMER_RIGHT_12_TM=58.874
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=993
PRIMER_PAIR_13_PENALTY=2.859231
PRIMER_LEFT_13_PENALTY=1.732988
PRIMER_RIGHT_13_PENALTY=1.126243
PRIMER_LEFT_13_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_13_SEQUENCE=TTTCCACAAACACATTCCGT
PRIMER_LEFT_13=926,20
PRIMER_RIGHT_13=1920,20
PRIMER_LEFT_13_TM=58.267
PRIMER_RIGHT_13_TM=58.874
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=995
PRIMER_PAIR_14_PENALTY=3.013450
PRIMER_LEFT_14_PENALTY=1.197845
PRIMER_RIGHT_14_PENALTY=1.815604
PRIMER_LEFT_14_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_14_SEQUENCE=AAGAATGTGGCATCTTCCTTG
PRIMER_LEFT_14=873,20
PRIMER_RIGHT_14=1792,21
PRIMER_LEFT_14_TM=58.802
PRIMER_RIGHT_14_TM=59.184
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=920
PRIMER_PAIR_15_PENALTY=3.063170
PRIMER_LEFT_15_PENALTY=1.247566
PRIMER_RIGHT_15_PENALTY=1.815604
PRIMER_LEFT_15_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_15_SEQUENCE=AAGAATGTGGCATCTTCCTTG
PRIMER_LEFT_15=872,21
PRIMER_RIGHT_15=1792,21
PRIMER_LEFT_15_TM=60.248
PRIMER_RIGHT_15_TM=59.184
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.1000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=921
PRIMER_PAIR_16_PENALTY=3.231671
PRIMER_LEFT_16_PENALTY=1.197845
PRIMER_RIGHT_16_PENALTY=2.033826
PRIMER_LEFT_16_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_16_SEQUENCE=ATCGAATCTGCACCACTCCTT
PRIMER_LEFT_16=873,20
PRIMER_RIGHT_16=1606,21
PRIMER_LEFT_16_TM=58.802
PRIMER_RIGHT_16_TM=61.034
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.1000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=734
PRIMER_PAIR_17_PENALTY=3.261992
PRIMER_LEFT_17_PENALTY=1.446387
PRIMER_RIGHT_17_PENALTY=1.815604
PRIMER_LEFT_17_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_17_SEQUENCE=AAGAATGTGGCATCTTCCTTG
PRIMER_LEFT_17=928,20
PRIMER_RIGHT_17=1792,21
PRIMER_LEFT_17_TM=58.554
PRIMER_RIGHT_17_TM=59.184
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=865
PRIMER_PAIR_18_PENALTY=3.281392
PRIMER_LEFT_18_PENALTY=1.247566
PRIMER_RIGHT_18_PENALTY=2.033826
PRIMER_LEFT_18_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_18_SEQUENCE=ATCGAATCTGCACCACTCCTT
PRIMER_LEFT_18=872,21
PRIMER_RIGHT_18=1606,21
PRIMER_LEFT_18_TM=60.248
PRIMER_RIGHT_18_TM=61.034
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.1000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=735
PRIMER_PAIR_19_PENALTY=3.284184
PRIMER_LEFT_19_PENALTY=1.197845
PRIMER_RIGHT_19_PENALTY=2.086339
PRIMER_LEFT_19_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_19_SEQUENCE=TCTGCACCACTCCTTAAACTGA
PRIMER_LEFT_19=873,20
PRIMER_RIGHT_19=1600,22
PRIMER_LEFT_19_TM=58.802
PRIMER_RIGHT_19_TM=59.914
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.455
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.1000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=728
PRIMER_PAIR_20_PENALTY=3.333905
PRIMER_LEFT_20_PENALTY=1.247566
PRIMER_RIGHT_20_PENALTY=2.086339
PRIMER_LEFT_20_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_20_SEQUENCE=TCTGCACCACTCCTTAAACTGA
PRIMER_LEFT_20=872,21
PRIMER_RIGHT_20=1600,22
PRIMER_LEFT_20_TM=60.248
PRIMER_RIGHT_20_TM=59.914
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.1000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=729
PRIMER_PAIR_21_PENALTY=3.335097
PRIMER_LEFT_21_PENALTY=3.180219
PRIMER_RIGHT_21_PENALTY=0.154878
PRIMER_LEFT_21_SEQUENCE=CTTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_21_SEQUENCE=GCATCTTCCTTGCCGTTTAG
PRIMER_LEFT_21=871,22
PRIMER_RIGHT_21=1783,20
PRIMER_LEFT_21_TM=61.180
PRIMER_RIGHT_21_TM=59.845
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.1000
PRIMER_RIGHT_21_END_STABILITY=6.3000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=913
PRIMER_PAIR_22_PENALTY=3.413061
PRIMER_LEFT_22_PENALTY=1.197845
PRIMER_RIGHT_22_PENALTY=2.215215
PRIMER_LEFT_22_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_22_SEQUENCE=TGCACCACTCCTTAAACTGAAA
PRIMER_LEFT_22=873,20
PRIMER_RIGHT_22=1598,22
PRIMER_LEFT_22_TM=58.802
PRIMER_RIGHT_22_TM=59.785
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.1000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=726
PRIMER_PAIR_23_PENALTY=3.460772
PRIMER_LEFT_23_PENALTY=1.197845
PRIMER_RIGHT_23_PENALTY=2.262926
PRIMER_LEFT_23_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_23_SEQUENCE=AGAATGTGGCATCTTCCTTG
PRIMER_LEFT_23=873,20
PRIMER_RIGHT_23=1791,20
PRIMER_LEFT_23_TM=58.802
PRIMER_RIGHT_23_TM=57.737
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.1000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=919
PRIMER_PAIR_24_PENALTY=3.462781
PRIMER_LEFT_24_PENALTY=1.247566
PRIMER_RIGHT_24_PENALTY=2.215215
PRIMER_LEFT_24_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_24_SEQUENCE=TGCACCACTCCTTAAACTGAAA
PRIMER_LEFT_24=872,21
PRIMER_RIGHT_24=1598,22
PRIMER_LEFT_24_TM=60.248
PRIMER_RIGHT_24_TM=59.785
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.1000
PRIMER_RIGHT_24_END_STABILITY=7.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=727
PRIMER_PAIR_25_PENALTY=3.480213
PRIMER_LEFT_25_PENALTY=1.446387
PRIMER_RIGHT_25_PENALTY=2.033826
PRIMER_LEFT_25_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_25_SEQUENCE=ATCGAATCTGCACCACTCCTT
PRIMER_LEFT_25=928,20
PRIMER_RIGHT_25=1606,21
PRIMER_LEFT_25_TM=58.554
PRIMER_RIGHT_25_TM=61.034
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=679
PRIMER_PAIR_26_PENALTY=3.495057
PRIMER_LEFT_26_PENALTY=3.180219
PRIMER_RIGHT_26_PENALTY=0.314838
PRIMER_LEFT_26_SEQUENCE=CTTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_26_SEQUENCE=ATCGAATCTGCACCACTCCT
PRIMER_LEFT_26=871,22
PRIMER_RIGHT_26=1606,20
PRIMER_LEFT_26_TM=61.180
PRIMER_RIGHT_26_TM=59.685
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.1000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=736
PRIMER_PAIR_27_PENALTY=3.510492
PRIMER_LEFT_27_PENALTY=1.247566
PRIMER_RIGHT_27_PENALTY=2.262926
PRIMER_LEFT_27_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_27_SEQUENCE=AGAATGTGGCATCTTCCTTG
PRIMER_LEFT_27=872,21
PRIMER_RIGHT_27=1791,20
PRIMER_LEFT_27_TM=60.248
PRIMER_RIGHT_27_TM=57.737
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.1000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=920
PRIMER_PAIR_28_PENALTY=3.513183
PRIMER_LEFT_28_PENALTY=1.197845
PRIMER_RIGHT_28_PENALTY=2.315338
PRIMER_LEFT_28_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_28_SEQUENCE=AATGTGGCATCTTCCTTGCC
PRIMER_LEFT_28=873,20
PRIMER_RIGHT_28=1789,20
PRIMER_LEFT_28_TM=58.802
PRIMER_RIGHT_28_TM=62.315
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.1000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=917
PRIMER_PAIR_29_PENALTY=3.532726
PRIMER_LEFT_29_PENALTY=1.446387
PRIMER_RIGHT_29_PENALTY=2.086339
PRIMER_LEFT_29_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_29_SEQUENCE=TCTGCACCACTCCTTAAACTGA
PRIMER_LEFT_29=928,20
PRIMER_RIGHT_29=1600,22
PRIMER_LEFT_29_TM=58.554
PRIMER_RIGHT_29_TM=59.914
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.4000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=673
PRIMER_PAIR_30_PENALTY=3.562904
PRIMER_LEFT_30_PENALTY=1.247566
PRIMER_RIGHT_30_PENALTY=2.315338
PRIMER_LEFT_30_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_30_SEQUENCE=AATGTGGCATCTTCCTTGCC
PRIMER_LEFT_30=872,21
PRIMER_RIGHT_30=1789,20
PRIMER_LEFT_30_TM=60.248
PRIMER_RIGHT_30_TM=62.315
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.1000
PRIMER_RIGHT_30_END_STABILITY=10.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=918
PRIMER_PAIR_31_PENALTY=3.709313
PRIMER_LEFT_31_PENALTY=1.446387
PRIMER_RIGHT_31_PENALTY=2.262926
PRIMER_LEFT_31_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_31_SEQUENCE=AGAATGTGGCATCTTCCTTG
PRIMER_LEFT_31=928,20
PRIMER_RIGHT_31=1791,20
PRIMER_LEFT_31_TM=58.554
PRIMER_RIGHT_31_TM=57.737
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=864
PRIMER_PAIR_32_PENALTY=3.761725
PRIMER_LEFT_32_PENALTY=1.446387
PRIMER_RIGHT_32_PENALTY=2.315338
PRIMER_LEFT_32_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_32_SEQUENCE=AATGTGGCATCTTCCTTGCC
PRIMER_LEFT_32=928,20
PRIMER_RIGHT_32=1789,20
PRIMER_LEFT_32_TM=58.554
PRIMER_RIGHT_32_TM=62.315
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=10.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=862
PRIMER_PAIR_33_PENALTY=3.766814
PRIMER_LEFT_33_PENALTY=1.732988
PRIMER_RIGHT_33_PENALTY=2.033826
PRIMER_LEFT_33_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_33_SEQUENCE=ATCGAATCTGCACCACTCCTT
PRIMER_LEFT_33=926,20
PRIMER_RIGHT_33=1606,21
PRIMER_LEFT_33_TM=58.267
PRIMER_RIGHT_33_TM=61.034
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=681
PRIMER_PAIR_34_PENALTY=3.819327
PRIMER_LEFT_34_PENALTY=1.732988
PRIMER_RIGHT_34_PENALTY=2.086339
PRIMER_LEFT_34_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_34_SEQUENCE=TCTGCACCACTCCTTAAACTGA
PRIMER_LEFT_34=926,20
PRIMER_RIGHT_34=1600,22
PRIMER_LEFT_34_TM=58.267
PRIMER_RIGHT_34_TM=59.914
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=675
PRIMER_PAIR_35_PENALTY=3.948203
PRIMER_LEFT_35_PENALTY=1.732988
PRIMER_RIGHT_35_PENALTY=2.215215
PRIMER_LEFT_35_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_35_SEQUENCE=TGCACCACTCCTTAAACTGAAA
PRIMER_LEFT_35=926,20
PRIMER_RIGHT_35=1598,22
PRIMER_LEFT_35_TM=58.267
PRIMER_RIGHT_35_TM=59.785
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=7.3000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=673
PRIMER_PAIR_36_PENALTY=3.981410
PRIMER_LEFT_36_PENALTY=3.180219
PRIMER_RIGHT_36_PENALTY=0.801192
PRIMER_LEFT_36_SEQUENCE=CTTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_36_SEQUENCE=TCGAATCTGCACCACTCCTT
PRIMER_LEFT_36=871,22
PRIMER_RIGHT_36=1605,20
PRIMER_LEFT_36_TM=61.180
PRIMER_RIGHT_36_TM=60.801
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.1000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=735
PRIMER_PAIR_37_PENALTY=4.048326
PRIMER_LEFT_37_PENALTY=1.732988
PRIMER_RIGHT_37_PENALTY=2.315338
PRIMER_LEFT_37_SEQUENCE=TAAGCCCAAAGTTGTTTCCA
PRIMER_RIGHT_37_SEQUENCE=AATGTGGCATCTTCCTTGCC
PRIMER_LEFT_37=926,20
PRIMER_RIGHT_37=1789,20
PRIMER_LEFT_37_TM=58.267
PRIMER_RIGHT_37_TM=62.315
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=10.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=864
PRIMER_PAIR_38_PENALTY=4.175123
PRIMER_LEFT_38_PENALTY=1.197845
PRIMER_RIGHT_38_PENALTY=2.977278
PRIMER_LEFT_38_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_38_SEQUENCE=CTGCACCACTCCTTAAACTGA
PRIMER_LEFT_38=873,20
PRIMER_RIGHT_38=1599,21
PRIMER_LEFT_38_TM=58.802
PRIMER_RIGHT_38_TM=58.023
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.1000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=727
PRIMER_PAIR_39_PENALTY=4.175123
PRIMER_LEFT_39_PENALTY=1.197845
PRIMER_RIGHT_39_PENALTY=2.977278
PRIMER_LEFT_39_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_39_SEQUENCE=TCTGCACCACTCCTTAAACTG
PRIMER_LEFT_39=873,20
PRIMER_RIGHT_39=1600,21
PRIMER_LEFT_39_TM=58.802
PRIMER_RIGHT_39_TM=58.023
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.1000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=728
PRIMER_PAIR_40_PENALTY=4.224844
PRIMER_LEFT_40_PENALTY=1.247566
PRIMER_RIGHT_40_PENALTY=2.977278
PRIMER_LEFT_40_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_40_SEQUENCE=CTGCACCACTCCTTAAACTGA
PRIMER_LEFT_40=872,21
PRIMER_RIGHT_40=1599,21
PRIMER_LEFT_40_TM=60.248
PRIMER_RIGHT_40_TM=58.023
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.1000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=728
PRIMER_PAIR_41_PENALTY=4.224844
PRIMER_LEFT_41_PENALTY=1.247566
PRIMER_RIGHT_41_PENALTY=2.977278
PRIMER_LEFT_41_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_41_SEQUENCE=TCTGCACCACTCCTTAAACTG
PRIMER_LEFT_41=872,21
PRIMER_RIGHT_41=1600,21
PRIMER_LEFT_41_TM=60.248
PRIMER_RIGHT_41_TM=58.023
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.1000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=729
PRIMER_PAIR_42_PENALTY=4.274941
PRIMER_LEFT_42_PENALTY=4.120062
PRIMER_RIGHT_42_PENALTY=0.154878
PRIMER_LEFT_42_SEQUENCE=ATAAGCCCAAAGTTGTTTCC
PRIMER_RIGHT_42_SEQUENCE=GCATCTTCCTTGCCGTTTAG
PRIMER_LEFT_42=925,20
PRIMER_RIGHT_42=1783,20
PRIMER_LEFT_42_TM=55.880
PRIMER_RIGHT_42_TM=59.845
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=859
PRIMER_PAIR_43_PENALTY=4.306600
PRIMER_LEFT_43_PENALTY=1.197845
PRIMER_RIGHT_43_PENALTY=3.108755
PRIMER_LEFT_43_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_43_SEQUENCE=TGCACCACTCCTTAAACTGA
PRIMER_LEFT_43=873,20
PRIMER_RIGHT_43=1598,20
PRIMER_LEFT_43_TM=58.802
PRIMER_RIGHT_43_TM=56.891
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.1000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=726
PRIMER_PAIR_44_PENALTY=4.356321
PRIMER_LEFT_44_PENALTY=1.247566
PRIMER_RIGHT_44_PENALTY=3.108755
PRIMER_LEFT_44_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_44_SEQUENCE=TGCACCACTCCTTAAACTGA
PRIMER_LEFT_44=872,21
PRIMER_RIGHT_44=1598,20
PRIMER_LEFT_44_TM=60.248
PRIMER_RIGHT_44_TM=56.891
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.1000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=727
PRIMER_PAIR_45_PENALTY=4.376271
PRIMER_LEFT_45_PENALTY=1.197845
PRIMER_RIGHT_45_PENALTY=3.178426
PRIMER_LEFT_45_SEQUENCE=TGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_45_SEQUENCE=ATCTGCACCACTCCTTAAACTGA
PRIMER_LEFT_45=873,20
PRIMER_RIGHT_45=1601,23
PRIMER_LEFT_45_TM=58.802
PRIMER_RIGHT_45_TM=60.178
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=43.478
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.1000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=729
PRIMER_PAIR_46_PENALTY=4.423665
PRIMER_LEFT_46_PENALTY=1.446387
PRIMER_RIGHT_46_PENALTY=2.977278
PRIMER_LEFT_46_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_46_SEQUENCE=CTGCACCACTCCTTAAACTGA
PRIMER_LEFT_46=928,20
PRIMER_RIGHT_46=1599,21
PRIMER_LEFT_46_TM=58.554
PRIMER_RIGHT_46_TM=58.023
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.4000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=672
PRIMER_PAIR_47_PENALTY=4.423665
PRIMER_LEFT_47_PENALTY=1.446387
PRIMER_RIGHT_47_PENALTY=2.977278
PRIMER_LEFT_47_SEQUENCE=AGCCCAAAGTTGTTTCCATT
PRIMER_RIGHT_47_SEQUENCE=TCTGCACCACTCCTTAAACTG
PRIMER_LEFT_47=928,20
PRIMER_RIGHT_47=1600,21
PRIMER_LEFT_47_TM=58.554
PRIMER_RIGHT_47_TM=58.023
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.4000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=673
PRIMER_PAIR_48_PENALTY=4.425992
PRIMER_LEFT_48_PENALTY=1.247566
PRIMER_RIGHT_48_PENALTY=3.178426
PRIMER_LEFT_48_SEQUENCE=TTGTTGCATCTTCAGAGTCCC
PRIMER_RIGHT_48_SEQUENCE=ATCTGCACCACTCCTTAAACTGA
PRIMER_LEFT_48=872,21
PRIMER_RIGHT_48=1601,23
PRIMER_LEFT_48_TM=60.248
PRIMER_RIGHT_48_TM=60.178
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=43.478
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.1000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=730
PRIMER_PAIR_49_PENALTY=4.434900
PRIMER_LEFT_49_PENALTY=4.120062
PRIMER_RIGHT_49_PENALTY=0.314838
PRIMER_LEFT_49_SEQUENCE=ATAAGCCCAAAGTTGTTTCC
PRIMER_RIGHT_49_SEQUENCE=ATCGAATCTGCACCACTCCT
PRIMER_LEFT_49=925,20
PRIMER_RIGHT_49=1606,20
PRIMER_LEFT_49_TM=55.880
PRIMER_RIGHT_49_TM=59.685
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=682
=
SEQUENCE_ID=MAGIC.105_5_11057693_11057699_zu-0_ct-1_12839_13678_TRUE
SEQUENCE_TARGET=950,106
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGAATAACACCCGAGCCAAGTATCGTTTATCTNAGGTGTTCCCNTGGGTCACAATGTTTCATCGCACCCGNCTTAGGGTGTGTTTCCTTGAGTAACACCCGAACCGGAACAACACCNANCCTAGCTCCCGAAGTGCTGATGGTATCCNTTTGACCGGCTCNGGTACTTGGGTGAAATCCGACCCCNACAAAANATAAAAACATTTAGCGNTTNNNNNCATTTTANGACTGTTAGAGNTTNGAGCANTCTCTAGTTACTTTAGTTCTTGTGATTTGTCATGTGTTCTAGAGTTTAAANAGTTAGCATTAAAGGTAAACTAGTTACTGCTAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_12714915_12715228_no-0_wil-2_20559_51062_TRUE
SEQUENCE_TARGET=950,413
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_6381249_6381508_wil-2_edi-0_68770_436157_TRUE
SEQUENCE_TARGET=950,359
SEQUENCE_TEMPLATE=GACTCATGANTTCAAATGCAACTTATTTTAAGGTTGATCAGTGTTTCAGTTTCTTTTCCTAAACTAATGTTCGAGCACATAGGCTTAANACCTGCCATATAGCAAAANNATNNNNNTNTCTTCGTTTATTTTNTTTTTGGNAAGACTAACACAATAGCATNTNTTTAAGGTATCAATCTTGGTGGTTTCATTTGATCAATATGAAAAGGCNGTCGCTAGAAACATTTCAACAAAAGTTTCGATGTGATGTAGTACAATTTACGAGTAATCTTTCAAAANTTGCTAGTTAATGATCGGNAATTTNTCTTTCTCAGAAACTTTCAAAGATTTTTGATGATNCATTCATTNAAAATCTATATAAAAACATTTATGANGGACTTATACGTAAATCTCTAAAAATTCCGTCAAAAATCAAAAATATTTCTTGTTGTANGACCNAACTAAATGAANNTCAGTATATAGATTATTGGGACAAGAAGTCAAGATGTTGTTGGNANGTTCTCATNNTCNATTTTATCATTGTTTTACCNTCATGGATTTAATGTTGTTCCAATATTATAGATNCAAATNAACACACACGTANATAACTATGTTTCTGTTATTGAATTAATACANAATTATTAAAACAATNATTTACTTNTGATCTCTTCTTCTTCTTACGTNAATGAANGNANNCTGATAGAGNCTTGCGATATNGCAATCTCCTAANACATAATTATNCCCCTACTTTGNTGTAAGTAGATTCTATGGGTGTNNATCTTNCANNATACAACTATCAATCTNTNTATTAACACCTNNNAANATCCTANCAAATCTACTTTGAGTTTCTGANAAATTNATTTTGGAGAANAANTAGATTTTTCTATCGTATNTTCNTNTNTGTTGNGATGTCTTTTCACAACCAATCGTANATGTGTATTTTNCTTAATCGACNCANCTCTTTGNTTTTATGTAAATATNCATATNTTTATNNNNNNNNNNNNATAAACTCCAACAATACNCNACATTTTCTTATTCAAACTNAATATTTAAATGATAAGAATACNGATTCTCAGGTTATGTCGNAANTTTGGTTTAAATCTCATGGTTAACAAAAACAAATGAAAATCCTAAAGTAATAGAAAATGATGTAACTTGCGAANTTAGATCAACATTAACATACCANGACTCTTGACCNNNNNTCCATAGGAATACTAAGGCAATTAGTNNNCCATGNTANTTTNATNTATTTTTAATCGTTAAGNGANAAAANTACTATTNAAAANTCTAANTTCTANATANTTTATGCCATTGAAATGAAANNNNTNNNNNNNNANAAAAGNNNTCAAAAGTATAGGAAAGAAGACCGACAATTGCCAAGAGGTAACCCCGAGTTCAGCAAGCTTAGCATCCGAAGTTTGTCTCATGACCTTGACTCCATGAATTTCAGTGGGAACCTCAGGAGCGAATATCGAATCATGATTAAGTTCCATAGTTGAAACTCAAACTAAATGATGAACAAAGAAGAGATGAATNNNNNNNNNNNNNNNNNNNATGTGTCCATATTTAAAACCATCGCGTAACAACGATAGTNGGGTAACATTGNCGTAAATGAAATAAGGTTGTACGTCCTGATAATAACACATNGAACTACATGACAACAGAGAGAAAGTTGTGGTTGCTATAANNNTAGTACTCGTAAATAGTGCCGTCCCATGTTTTTAGTGTGGCTAGTACTGANNTTTTTAGGGACACCGAAGATATTANTACAATCGAAATATTTGTAATGTCACTATAGAAAAAGGACGAANTTAATTGAGACTCTCCCGACACATGAAAAATCTCATGAATATANTTTTGATGTTGTCGTTATCAGTTTTTGATTGTGTTAAATATGTAAAGTAGATAGATGATAAAATTCACAAGATTGTTATACGAGTCCTATATTTCATGCAGGTCGCAAGGNCATGATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.445813
PRIMER_LEFT_0_PENALTY=3.216632
PRIMER_RIGHT_0_PENALTY=0.229181
PRIMER_LEFT_0_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_0_SEQUENCE=CTCCTGAGGTTCCCACTGAA
PRIMER_LEFT_0=466,21
PRIMER_RIGHT_0=1443,20
PRIMER_LEFT_0_TM=57.783
PRIMER_RIGHT_0_TM=60.229
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=978
PRIMER_PAIR_1_PENALTY=3.471246
PRIMER_LEFT_1_PENALTY=3.216632
PRIMER_RIGHT_1_PENALTY=0.254613
PRIMER_LEFT_1_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_1_SEQUENCE=CTCTTGGCAATTGTCGGTCT
PRIMER_LEFT_1=466,21
PRIMER_RIGHT_1=1361,20
PRIMER_LEFT_1_TM=57.783
PRIMER_RIGHT_1_TM=60.255
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=896
PRIMER_PAIR_2_PENALTY=3.841121
PRIMER_LEFT_2_PENALTY=3.216632
PRIMER_RIGHT_2_PENALTY=0.624489
PRIMER_LEFT_2_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_2_SEQUENCE=TCCTGAGGTTCCCACTGAAA
PRIMER_LEFT_2=466,21
PRIMER_RIGHT_2=1442,20
PRIMER_LEFT_2_TM=57.783
PRIMER_RIGHT_2_TM=60.624
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=977
PRIMER_PAIR_3_PENALTY=3.852665
PRIMER_LEFT_3_PENALTY=3.216632
PRIMER_RIGHT_3_PENALTY=0.636033
PRIMER_LEFT_3_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_3_SEQUENCE=TTGGCAATTGTCGGTCTTCT
PRIMER_LEFT_3=466,21
PRIMER_RIGHT_3=1358,20
PRIMER_LEFT_3_TM=57.783
PRIMER_RIGHT_3_TM=60.636
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=893
PRIMER_PAIR_4_PENALTY=3.852665
PRIMER_LEFT_4_PENALTY=3.216632
PRIMER_RIGHT_4_PENALTY=0.636033
PRIMER_LEFT_4_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_4_SEQUENCE=CTTGGCAATTGTCGGTCTTC
PRIMER_LEFT_4=466,21
PRIMER_RIGHT_4=1359,20
PRIMER_LEFT_4_TM=57.783
PRIMER_RIGHT_4_TM=60.636
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=894
PRIMER_PAIR_5_PENALTY=3.852665
PRIMER_LEFT_5_PENALTY=3.216632
PRIMER_RIGHT_5_PENALTY=0.636033
PRIMER_LEFT_5_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_5_SEQUENCE=TCTTGGCAATTGTCGGTCTT
PRIMER_LEFT_5=466,21
PRIMER_RIGHT_5=1360,20
PRIMER_LEFT_5_TM=57.783
PRIMER_RIGHT_5_TM=60.636
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=895
PRIMER_PAIR_6_PENALTY=3.924141
PRIMER_LEFT_6_PENALTY=3.216632
PRIMER_RIGHT_6_PENALTY=0.707509
PRIMER_LEFT_6_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_6_SEQUENCE=CGATATTCGCTCCTGAGGTT
PRIMER_LEFT_6=466,21
PRIMER_RIGHT_6=1452,20
PRIMER_LEFT_6_TM=57.783
PRIMER_RIGHT_6_TM=59.292
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=987
PRIMER_PAIR_7_PENALTY=4.042430
PRIMER_LEFT_7_PENALTY=3.216632
PRIMER_RIGHT_7_PENALTY=0.825798
PRIMER_LEFT_7_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_7_SEQUENCE=GGCAATTGTCGGTCTTCTTT
PRIMER_LEFT_7=466,21
PRIMER_RIGHT_7=1356,20
PRIMER_LEFT_7_TM=57.783
PRIMER_RIGHT_7_TM=59.174
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=891
PRIMER_PAIR_8_PENALTY=4.049363
PRIMER_LEFT_8_PENALTY=3.820182
PRIMER_RIGHT_8_PENALTY=0.229181
PRIMER_LEFT_8_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_8_SEQUENCE=CTCCTGAGGTTCCCACTGAA
PRIMER_LEFT_8=467,20
PRIMER_RIGHT_8=1443,20
PRIMER_LEFT_8_TM=56.180
PRIMER_RIGHT_8_TM=60.229
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=977
PRIMER_PAIR_9_PENALTY=4.054998
PRIMER_LEFT_9_PENALTY=3.825817
PRIMER_RIGHT_9_PENALTY=0.229181
PRIMER_LEFT_9_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_9_SEQUENCE=CTCCTGAGGTTCCCACTGAA
PRIMER_LEFT_9=465,22
PRIMER_RIGHT_9=1443,20
PRIMER_LEFT_9_TM=58.174
PRIMER_RIGHT_9_TM=60.229
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=979
PRIMER_PAIR_10_PENALTY=4.074796
PRIMER_LEFT_10_PENALTY=3.820182
PRIMER_RIGHT_10_PENALTY=0.254613
PRIMER_LEFT_10_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_10_SEQUENCE=CTCTTGGCAATTGTCGGTCT
PRIMER_LEFT_10=467,20
PRIMER_RIGHT_10=1361,20
PRIMER_LEFT_10_TM=56.180
PRIMER_RIGHT_10_TM=60.255
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=895
PRIMER_PAIR_11_PENALTY=4.080430
PRIMER_LEFT_11_PENALTY=3.825817
PRIMER_RIGHT_11_PENALTY=0.254613
PRIMER_LEFT_11_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_11_SEQUENCE=CTCTTGGCAATTGTCGGTCT
PRIMER_LEFT_11=465,22
PRIMER_RIGHT_11=1361,20
PRIMER_LEFT_11_TM=58.174
PRIMER_RIGHT_11_TM=60.255
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=897
PRIMER_PAIR_12_PENALTY=4.232418
PRIMER_LEFT_12_PENALTY=3.216632
PRIMER_RIGHT_12_PENALTY=1.015786
PRIMER_LEFT_12_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_12_SEQUENCE=TCATGATTCGATATTCGCTCC
PRIMER_LEFT_12=466,21
PRIMER_RIGHT_12=1460,21
PRIMER_LEFT_12_TM=57.783
PRIMER_RIGHT_12_TM=60.016
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=995
PRIMER_PAIR_13_PENALTY=4.396415
PRIMER_LEFT_13_PENALTY=3.216632
PRIMER_RIGHT_13_PENALTY=1.179782
PRIMER_LEFT_13_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_13_SEQUENCE=TGATTCGATATTCGCTCCTG
PRIMER_LEFT_13=466,21
PRIMER_RIGHT_13=1457,20
PRIMER_LEFT_13_TM=57.783
PRIMER_RIGHT_13_TM=58.820
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=992
PRIMER_PAIR_14_PENALTY=4.444671
PRIMER_LEFT_14_PENALTY=3.820182
PRIMER_RIGHT_14_PENALTY=0.624489
PRIMER_LEFT_14_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_14_SEQUENCE=TCCTGAGGTTCCCACTGAAA
PRIMER_LEFT_14=467,20
PRIMER_RIGHT_14=1442,20
PRIMER_LEFT_14_TM=56.180
PRIMER_RIGHT_14_TM=60.624
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=976
PRIMER_PAIR_15_PENALTY=4.450306
PRIMER_LEFT_15_PENALTY=3.825817
PRIMER_RIGHT_15_PENALTY=0.624489
PRIMER_LEFT_15_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_15_SEQUENCE=TCCTGAGGTTCCCACTGAAA
PRIMER_LEFT_15=465,22
PRIMER_RIGHT_15=1442,20
PRIMER_LEFT_15_TM=58.174
PRIMER_RIGHT_15_TM=60.624
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.3000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=978
PRIMER_PAIR_16_PENALTY=4.456215
PRIMER_LEFT_16_PENALTY=3.820182
PRIMER_RIGHT_16_PENALTY=0.636033
PRIMER_LEFT_16_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_16_SEQUENCE=TTGGCAATTGTCGGTCTTCT
PRIMER_LEFT_16=467,20
PRIMER_RIGHT_16=1358,20
PRIMER_LEFT_16_TM=56.180
PRIMER_RIGHT_16_TM=60.636
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=892
PRIMER_PAIR_17_PENALTY=4.456215
PRIMER_LEFT_17_PENALTY=3.820182
PRIMER_RIGHT_17_PENALTY=0.636033
PRIMER_LEFT_17_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_17_SEQUENCE=CTTGGCAATTGTCGGTCTTC
PRIMER_LEFT_17=467,20
PRIMER_RIGHT_17=1359,20
PRIMER_LEFT_17_TM=56.180
PRIMER_RIGHT_17_TM=60.636
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=893
PRIMER_PAIR_18_PENALTY=4.456215
PRIMER_LEFT_18_PENALTY=3.820182
PRIMER_RIGHT_18_PENALTY=0.636033
PRIMER_LEFT_18_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_18_SEQUENCE=TCTTGGCAATTGTCGGTCTT
PRIMER_LEFT_18=467,20
PRIMER_RIGHT_18=1360,20
PRIMER_LEFT_18_TM=56.180
PRIMER_RIGHT_18_TM=60.636
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=894
PRIMER_PAIR_19_PENALTY=4.461850
PRIMER_LEFT_19_PENALTY=3.825817
PRIMER_RIGHT_19_PENALTY=0.636033
PRIMER_LEFT_19_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_19_SEQUENCE=TTGGCAATTGTCGGTCTTCT
PRIMER_LEFT_19=465,22
PRIMER_RIGHT_19=1358,20
PRIMER_LEFT_19_TM=58.174
PRIMER_RIGHT_19_TM=60.636
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=894
PRIMER_PAIR_20_PENALTY=4.461850
PRIMER_LEFT_20_PENALTY=3.825817
PRIMER_RIGHT_20_PENALTY=0.636033
PRIMER_LEFT_20_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_20_SEQUENCE=CTTGGCAATTGTCGGTCTTC
PRIMER_LEFT_20=465,22
PRIMER_RIGHT_20=1359,20
PRIMER_LEFT_20_TM=58.174
PRIMER_RIGHT_20_TM=60.636
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=895
PRIMER_PAIR_21_PENALTY=4.461850
PRIMER_LEFT_21_PENALTY=3.825817
PRIMER_RIGHT_21_PENALTY=0.636033
PRIMER_LEFT_21_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_21_SEQUENCE=TCTTGGCAATTGTCGGTCTT
PRIMER_LEFT_21=465,22
PRIMER_RIGHT_21=1360,20
PRIMER_LEFT_21_TM=58.174
PRIMER_RIGHT_21_TM=60.636
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=896
PRIMER_PAIR_22_PENALTY=4.527691
PRIMER_LEFT_22_PENALTY=3.820182
PRIMER_RIGHT_22_PENALTY=0.707509
PRIMER_LEFT_22_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_22_SEQUENCE=CGATATTCGCTCCTGAGGTT
PRIMER_LEFT_22=467,20
PRIMER_RIGHT_22=1452,20
PRIMER_LEFT_22_TM=56.180
PRIMER_RIGHT_22_TM=59.292
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=986
PRIMER_PAIR_23_PENALTY=4.533326
PRIMER_LEFT_23_PENALTY=3.825817
PRIMER_RIGHT_23_PENALTY=0.707509
PRIMER_LEFT_23_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_23_SEQUENCE=CGATATTCGCTCCTGAGGTT
PRIMER_LEFT_23=465,22
PRIMER_RIGHT_23=1452,20
PRIMER_LEFT_23_TM=58.174
PRIMER_RIGHT_23_TM=59.292
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=4.645980
PRIMER_LEFT_24_PENALTY=3.820182
PRIMER_RIGHT_24_PENALTY=0.825798
PRIMER_LEFT_24_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_24_SEQUENCE=GGCAATTGTCGGTCTTCTTT
PRIMER_LEFT_24=467,20
PRIMER_RIGHT_24=1356,20
PRIMER_LEFT_24_TM=56.180
PRIMER_RIGHT_24_TM=59.174
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=890
PRIMER_PAIR_25_PENALTY=4.651615
PRIMER_LEFT_25_PENALTY=3.825817
PRIMER_RIGHT_25_PENALTY=0.825798
PRIMER_LEFT_25_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_25_SEQUENCE=GGCAATTGTCGGTCTTCTTT
PRIMER_LEFT_25=465,22
PRIMER_RIGHT_25=1356,20
PRIMER_LEFT_25_TM=58.174
PRIMER_RIGHT_25_TM=59.174
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=892
PRIMER_PAIR_26_PENALTY=4.695073
PRIMER_LEFT_26_PENALTY=3.216632
PRIMER_RIGHT_26_PENALTY=1.478440
PRIMER_LEFT_26_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_26_SEQUENCE=TATTCGCTCCTGAGGTTCCC
PRIMER_LEFT_26=466,21
PRIMER_RIGHT_26=1449,20
PRIMER_LEFT_26_TM=57.783
PRIMER_RIGHT_26_TM=61.478
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=984
PRIMER_PAIR_27_PENALTY=4.835968
PRIMER_LEFT_27_PENALTY=3.820182
PRIMER_RIGHT_27_PENALTY=1.015786
PRIMER_LEFT_27_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_27_SEQUENCE=TCATGATTCGATATTCGCTCC
PRIMER_LEFT_27=467,20
PRIMER_RIGHT_27=1460,21
PRIMER_LEFT_27_TM=56.180
PRIMER_RIGHT_27_TM=60.016
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.6000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=994
PRIMER_PAIR_28_PENALTY=4.841603
PRIMER_LEFT_28_PENALTY=3.825817
PRIMER_RIGHT_28_PENALTY=1.015786
PRIMER_LEFT_28_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_28_SEQUENCE=TCATGATTCGATATTCGCTCC
PRIMER_LEFT_28=465,22
PRIMER_RIGHT_28=1460,21
PRIMER_LEFT_28_TM=58.174
PRIMER_RIGHT_28_TM=60.016
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=996
PRIMER_PAIR_29_PENALTY=4.971113
PRIMER_LEFT_29_PENALTY=3.216632
PRIMER_RIGHT_29_PENALTY=1.754480
PRIMER_LEFT_29_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_29_SEQUENCE=TGGAGTCAAGGTCATGAGACA
PRIMER_LEFT_29=466,21
PRIMER_RIGHT_29=1418,21
PRIMER_LEFT_29_TM=57.783
PRIMER_RIGHT_29_TM=59.246
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=953
PRIMER_PAIR_30_PENALTY=4.999965
PRIMER_LEFT_30_PENALTY=3.820182
PRIMER_RIGHT_30_PENALTY=1.179782
PRIMER_LEFT_30_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_30_SEQUENCE=TGATTCGATATTCGCTCCTG
PRIMER_LEFT_30=467,20
PRIMER_RIGHT_30=1457,20
PRIMER_LEFT_30_TM=56.180
PRIMER_RIGHT_30_TM=58.820
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.6000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=991
PRIMER_PAIR_31_PENALTY=5.005599
PRIMER_LEFT_31_PENALTY=3.825817
PRIMER_RIGHT_31_PENALTY=1.179782
PRIMER_LEFT_31_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_31_SEQUENCE=TGATTCGATATTCGCTCCTG
PRIMER_LEFT_31=465,22
PRIMER_RIGHT_31=1457,20
PRIMER_LEFT_31_TM=58.174
PRIMER_RIGHT_31_TM=58.820
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=993
PRIMER_PAIR_32_PENALTY=5.019227
PRIMER_LEFT_32_PENALTY=3.216632
PRIMER_RIGHT_32_PENALTY=1.802595
PRIMER_LEFT_32_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_32_SEQUENCE=AGGTCATGAGACAAACTTCGG
PRIMER_LEFT_32=466,21
PRIMER_RIGHT_32=1410,21
PRIMER_LEFT_32_TM=57.783
PRIMER_RIGHT_32_TM=59.197
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=10.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=945
PRIMER_PAIR_33_PENALTY=5.057060
PRIMER_LEFT_33_PENALTY=3.216632
PRIMER_RIGHT_33_PENALTY=1.840428
PRIMER_LEFT_33_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_33_SEQUENCE=ACCTCTTGGCAATTGTCGGT
PRIMER_LEFT_33=466,21
PRIMER_RIGHT_33=1363,20
PRIMER_LEFT_33_TM=57.783
PRIMER_RIGHT_33_TM=61.840
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.6000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=898
PRIMER_PAIR_34_PENALTY=5.064584
PRIMER_LEFT_34_PENALTY=3.216632
PRIMER_RIGHT_34_PENALTY=1.847952
PRIMER_LEFT_34_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_34_SEQUENCE=ATGATTCGATATTCGCTCCTG
PRIMER_LEFT_34=466,21
PRIMER_RIGHT_34=1458,21
PRIMER_LEFT_34_TM=57.783
PRIMER_RIGHT_34_TM=59.152
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.6000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=993
PRIMER_PAIR_35_PENALTY=5.084190
PRIMER_LEFT_35_PENALTY=3.216632
PRIMER_RIGHT_35_PENALTY=1.867557
PRIMER_LEFT_35_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_35_SEQUENCE=CATGATTCGATATTCGCTCC
PRIMER_LEFT_35=466,21
PRIMER_RIGHT_35=1459,20
PRIMER_LEFT_35_TM=57.783
PRIMER_RIGHT_35_TM=58.132
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=994
PRIMER_PAIR_36_PENALTY=5.087256
PRIMER_LEFT_36_PENALTY=3.216632
PRIMER_RIGHT_36_PENALTY=1.870624
PRIMER_LEFT_36_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_36_SEQUENCE=GGTCATGAGACAAACTTCGG
PRIMER_LEFT_36=466,21
PRIMER_RIGHT_36=1409,20
PRIMER_LEFT_36_TM=57.783
PRIMER_RIGHT_36_TM=58.129
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=10.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=944
PRIMER_PAIR_37_PENALTY=5.199320
PRIMER_LEFT_37_PENALTY=3.216632
PRIMER_RIGHT_37_PENALTY=1.982688
PRIMER_LEFT_37_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_37_SEQUENCE=GGCAATTGTCGGTCTTCTTTC
PRIMER_LEFT_37=466,21
PRIMER_RIGHT_37=1356,21
PRIMER_LEFT_37_TM=57.783
PRIMER_RIGHT_37_TM=60.983
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.6000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=891
PRIMER_PAIR_38_PENALTY=5.298623
PRIMER_LEFT_38_PENALTY=3.820182
PRIMER_RIGHT_38_PENALTY=1.478440
PRIMER_LEFT_38_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_38_SEQUENCE=TATTCGCTCCTGAGGTTCCC
PRIMER_LEFT_38=467,20
PRIMER_RIGHT_38=1449,20
PRIMER_LEFT_38_TM=56.180
PRIMER_RIGHT_38_TM=61.478
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=983
PRIMER_PAIR_39_PENALTY=5.304257
PRIMER_LEFT_39_PENALTY=3.825817
PRIMER_RIGHT_39_PENALTY=1.478440
PRIMER_LEFT_39_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_39_SEQUENCE=TATTCGCTCCTGAGGTTCCC
PRIMER_LEFT_39=465,22
PRIMER_RIGHT_39=1449,20
PRIMER_LEFT_39_TM=58.174
PRIMER_RIGHT_39_TM=61.478
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=985
PRIMER_PAIR_40_PENALTY=5.311321
PRIMER_LEFT_40_PENALTY=3.216632
PRIMER_RIGHT_40_PENALTY=2.094688
PRIMER_LEFT_40_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_40_SEQUENCE=TCGATATTCGCTCCTGAGGTT
PRIMER_LEFT_40=466,21
PRIMER_RIGHT_40=1453,21
PRIMER_LEFT_40_TM=57.783
PRIMER_RIGHT_40_TM=61.095
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.6000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=988
PRIMER_PAIR_41_PENALTY=5.574663
PRIMER_LEFT_41_PENALTY=3.820182
PRIMER_RIGHT_41_PENALTY=1.754480
PRIMER_LEFT_41_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_41_SEQUENCE=TGGAGTCAAGGTCATGAGACA
PRIMER_LEFT_41=467,20
PRIMER_RIGHT_41=1418,21
PRIMER_LEFT_41_TM=56.180
PRIMER_RIGHT_41_TM=59.246
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.6000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=952
PRIMER_PAIR_42_PENALTY=5.580297
PRIMER_LEFT_42_PENALTY=3.825817
PRIMER_RIGHT_42_PENALTY=1.754480
PRIMER_LEFT_42_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_42_SEQUENCE=TGGAGTCAAGGTCATGAGACA
PRIMER_LEFT_42=465,22
PRIMER_RIGHT_42=1418,21
PRIMER_LEFT_42_TM=58.174
PRIMER_RIGHT_42_TM=59.246
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=954
PRIMER_PAIR_43_PENALTY=5.622777
PRIMER_LEFT_43_PENALTY=3.820182
PRIMER_RIGHT_43_PENALTY=1.802595
PRIMER_LEFT_43_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_43_SEQUENCE=AGGTCATGAGACAAACTTCGG
PRIMER_LEFT_43=467,20
PRIMER_RIGHT_43=1410,21
PRIMER_LEFT_43_TM=56.180
PRIMER_RIGHT_43_TM=59.197
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=10.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=944
PRIMER_PAIR_44_PENALTY=5.628412
PRIMER_LEFT_44_PENALTY=3.825817
PRIMER_RIGHT_44_PENALTY=1.802595
PRIMER_LEFT_44_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_44_SEQUENCE=AGGTCATGAGACAAACTTCGG
PRIMER_LEFT_44=465,22
PRIMER_RIGHT_44=1410,21
PRIMER_LEFT_44_TM=58.174
PRIMER_RIGHT_44_TM=59.197
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.6000
PRIMER_RIGHT_44_END_STABILITY=10.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=946
PRIMER_PAIR_45_PENALTY=5.637404
PRIMER_LEFT_45_PENALTY=3.216632
PRIMER_RIGHT_45_PENALTY=2.420772
PRIMER_LEFT_45_SEQUENCE=TTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_45_SEQUENCE=ATGGAGTCAAGGTCATGAGACA
PRIMER_LEFT_45=466,21
PRIMER_RIGHT_45=1419,22
PRIMER_LEFT_45_TM=57.783
PRIMER_RIGHT_45_TM=59.579
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.6000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=954
PRIMER_PAIR_46_PENALTY=5.660610
PRIMER_LEFT_46_PENALTY=3.820182
PRIMER_RIGHT_46_PENALTY=1.840428
PRIMER_LEFT_46_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_46_SEQUENCE=ACCTCTTGGCAATTGTCGGT
PRIMER_LEFT_46=467,20
PRIMER_RIGHT_46=1363,20
PRIMER_LEFT_46_TM=56.180
PRIMER_RIGHT_46_TM=61.840
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.6000
PRIMER_RIGHT_46_END_STABILITY=9.6000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=897
PRIMER_PAIR_47_PENALTY=5.666245
PRIMER_LEFT_47_PENALTY=3.825817
PRIMER_RIGHT_47_PENALTY=1.840428
PRIMER_LEFT_47_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_47_SEQUENCE=ACCTCTTGGCAATTGTCGGT
PRIMER_LEFT_47=465,22
PRIMER_RIGHT_47=1363,20
PRIMER_LEFT_47_TM=58.174
PRIMER_RIGHT_47_TM=61.840
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=9.6000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=899
PRIMER_PAIR_48_PENALTY=5.668134
PRIMER_LEFT_48_PENALTY=3.820182
PRIMER_RIGHT_48_PENALTY=1.847952
PRIMER_LEFT_48_SEQUENCE=TGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_48_SEQUENCE=ATGATTCGATATTCGCTCCTG
PRIMER_LEFT_48=467,20
PRIMER_RIGHT_48=1458,21
PRIMER_LEFT_48_TM=56.180
PRIMER_RIGHT_48_TM=59.152
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=992
PRIMER_PAIR_49_PENALTY=5.673769
PRIMER_LEFT_49_PENALTY=3.825817
PRIMER_RIGHT_49_PENALTY=1.847952
PRIMER_LEFT_49_SEQUENCE=ATTGGGACAAGAAGTCAAGATG
PRIMER_RIGHT_49_SEQUENCE=ATGATTCGATATTCGCTCCTG
PRIMER_LEFT_49=465,22
PRIMER_RIGHT_49=1458,21
PRIMER_LEFT_49_TM=58.174
PRIMER_RIGHT_49_TM=59.152
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.6000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=994
=
SEQUENCE_ID=MAGIC.338_2_8410516_8411168_zu-0_po-0_74629_117458_TRUE
SEQUENCE_TARGET=950,752
SEQUENCE_TEMPLATE=TTATGGACCACTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACATAGCTNAAAAAATATAAGANAATACGAAGTAGACTCATAGGATTGGATTATATAAAATCAGTCTATTGACTTTAGGAACAAACAAAAGATATAATTTGATCTGTTTNGAATTTTGTTGGTAATCACCGATATGGATCTTGCAGTCCTGGATATATAATTAATAGTAAAATATGTTTGGCCAACATTAGGAAGAAATCGATGGGATGGAAATCGCCTTCAAAGGAATATATATTCCATTTAATACTTTTCAAATTCGAANTTTTTTGGACGGCCAATTTGTTAATTATAATTAGTGTGCAGCTTTATTATCTGAAGTTTTCTAGTTCTATCTACATGATATTTGAACGGTTTAGATAGAGATTTTTATGTCTGTCAGTTGTATAATATGTATCATNTACTATGACCAGTTGTACCAATGTGGGCAATGTGATCAATATAATGTAACCTACTAACTTATAACCTATGTATTTNNTGCAAAATAATTATGTATGAAGTAATTTTGAATTTATTTGAATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTAGCAAAATAACTTAGCTCAGTAAGCTTTCAGAATAAATAAAGTAGTCATTTTCTATCAGAAGATGACATAATTTAGTTTTTTCTTCTGTGCAAACAATGACATAACAAAAAACAAATGACATTACTTAGTTACGGTACTAGATTAATCTTGAAGTGGTATGTGGTCGCAACAATCTGAATCTTTTAGTAAAAGGCATGAGATTGTTGTGGCCTTGTGAGATAAGTCACATTTTGTTCCGGTTAAAATATGAAATTATTCAGTATTTTTTGTCGNAGACCAACCTATATATTAAAATTCTTTANANTTTTTTNTTAATACATTGTCTTGAATTTCCACGATTTCTTGCACATAATAGTTGTTTAGGATTTGATCATCTGATACGGTANCGTNCAGATCCAAATTTCCGATCAAACCGGTTTGATTTGGCTGAGTAATGATGTTTGCAATTGTTTTCTAATATGTAACCAAAAGTTGACCAAAACAGTCTAANTGATTTTTCATTAATTTTTTGCTCAGCACAAATGAGGATATACCCGAATAATTAAGAATTATATANNANNAAAAAACACNAATAATGCACTGANCGTTNACGAGAACGACATAATTAAACTAATTAAAGNTCTTAACGATTGTCGNCTCTCCAATGTTCTTATACACACACAAATATAAATATGATTAGTCCAATCTATATNGTCGAACAGTAATTTACAGCAGACTTAGATCGATCTTTCGTATAGAAGAAAGAGACGTTACCAATGACATTACTGAAATAANNNNNTTTTTTTTTTTAACTACATANNNNNATTAACACATTCTAATACANCATACATTAATACATATATACTCTCACTACGTGTAAATGATAGAAGACGATCAATATTGGCTAGAGGTCCATCTTTGGTTAATTGTTCCATATATGTAGATCTAAGCTATATATACATAATACTTTCGGANTNTTTTTGTATTTCTTAAAACATTAAATATGCAAATGTAAACGGAAACAAATCAAACAAAACTTTTAATTTGATGTCAAGAAAACGATATNATATTTTTTTATTTTAGCCCAAGCTGTTTTTGGGTGTTTATTAGTATANAATTTATATTTAATTGGAGCGAAGCCTACATATAGAAAGTTTAGAGCATCATGTNGACNTCATGTGAATTCTAGGTCCAAAATTATGTACACACTACATAAAATATCATAACATCAAANGAAAAACACATATACCACACANNAAAAAAGGGAAAAAAGAAAAGAAATAACCACNATCACTTAGTTTCTGATTCTGGACAAGTAGTTCATGAATCATCTAATATATTAGTCATTATTGATTTCTTAAAGANTTATTGATCAATTCATACATATATTTCTATTTCTAGCCAAATATATAATAGAGCTAGATTTTTATTTTTTTTGTTCATAAANAGAAAACAGCTTTAATTGATAACAGTTGTTCATTGTTATTTTTGCTACTTAGACNATGGATCATNCATGAAAAGGTACCAAGTAATTCAAACAGTACTGNTATTGGTAGATTTTAGATATATGCATAAATGTGGGGGACANAAAAAATATAGATACATAAATATAATGGAAATGGTAAAAGAACAAAAATAGATAAAACATAGATANAGGGAGAAAGAAAAGTACTTTTTTACTTAAGAATATATTTCTGTCCACAACAAGATTTGCTAGGACGAAAAATCAGNAACTATTTCTCAATTCTTCTATGTAGCTCTTTAAAATANTAAAGGTCATCCGATGACCGGCATCAAACCCTTATCCGCAACTTGATACGCACGCTTTGTTCCTTTGCGATCATGCGTGAAATCTTCTCATTCTCATGATGATAAACCTTCCTTATGTCTTGCTCCTTAATTTTTTTCTNTGTTAAAGTGCCTTGCTCCTCTTTGTAACTCAAAGCTCATCATTTNTACAAAAATTAGATCATTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.869421
PRIMER_LEFT_0_PENALTY=0.861164
PRIMER_RIGHT_0_PENALTY=0.008257
PRIMER_LEFT_0_SEQUENCE=ATTGTTGTGGCCTTGTGAGA
PRIMER_RIGHT_0_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_0=896,20
PRIMER_RIGHT_0=1779,20
PRIMER_LEFT_0_TM=59.139
PRIMER_RIGHT_0_TM=60.008
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=884
PRIMER_PAIR_1_PENALTY=0.869421
PRIMER_LEFT_1_PENALTY=0.861164
PRIMER_RIGHT_1_PENALTY=0.008257
PRIMER_LEFT_1_SEQUENCE=GATTGTTGTGGCCTTGTGAG
PRIMER_RIGHT_1_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_1=895,20
PRIMER_RIGHT_1=1779,20
PRIMER_LEFT_1_TM=59.139
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=885
PRIMER_PAIR_2_PENALTY=0.869421
PRIMER_LEFT_2_PENALTY=0.861164
PRIMER_RIGHT_2_PENALTY=0.008257
PRIMER_LEFT_2_SEQUENCE=AGATTGTTGTGGCCTTGTGA
PRIMER_RIGHT_2_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_2=894,20
PRIMER_RIGHT_2=1779,20
PRIMER_LEFT_2_TM=59.139
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=886
PRIMER_PAIR_3_PENALTY=0.869421
PRIMER_LEFT_3_PENALTY=0.861164
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=GAGATTGTTGTGGCCTTGTG
PRIMER_RIGHT_3_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_3=893,20
PRIMER_RIGHT_3=1779,20
PRIMER_LEFT_3_TM=59.139
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=887
PRIMER_PAIR_4_PENALTY=0.869421
PRIMER_LEFT_4_PENALTY=0.861164
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=TGAGATTGTTGTGGCCTTGT
PRIMER_RIGHT_4_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_4=892,20
PRIMER_RIGHT_4=1779,20
PRIMER_LEFT_4_TM=59.139
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=888
PRIMER_PAIR_5_PENALTY=1.022875
PRIMER_LEFT_5_PENALTY=1.014618
PRIMER_RIGHT_5_PENALTY=0.008257
PRIMER_LEFT_5_SEQUENCE=AAAGGCATGAGATTGTTGTGG
PRIMER_RIGHT_5_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_5=885,21
PRIMER_RIGHT_5=1779,20
PRIMER_LEFT_5_TM=59.985
PRIMER_RIGHT_5_TM=60.008
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=895
PRIMER_PAIR_6_PENALTY=1.171860
PRIMER_LEFT_6_PENALTY=1.163603
PRIMER_RIGHT_6_PENALTY=0.008257
PRIMER_LEFT_6_SEQUENCE=AGATTGTTGTGGCCTTGTGAG
PRIMER_RIGHT_6_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_6=894,21
PRIMER_RIGHT_6=1779,20
PRIMER_LEFT_6_TM=60.164
PRIMER_RIGHT_6_TM=60.008
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=886
PRIMER_PAIR_7_PENALTY=1.284990
PRIMER_LEFT_7_PENALTY=1.276733
PRIMER_RIGHT_7_PENALTY=0.008257
PRIMER_LEFT_7_SEQUENCE=TGTGGTCGCAACAATCTGAA
PRIMER_RIGHT_7_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_7=855,20
PRIMER_RIGHT_7=1779,20
PRIMER_LEFT_7_TM=61.277
PRIMER_RIGHT_7_TM=60.008
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=925
PRIMER_PAIR_8_PENALTY=1.438124
PRIMER_LEFT_8_PENALTY=1.429867
PRIMER_RIGHT_8_PENALTY=0.008257
PRIMER_LEFT_8_SEQUENCE=ATGAGATTGTTGTGGCCTTG
PRIMER_RIGHT_8_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_8=891,20
PRIMER_RIGHT_8=1779,20
PRIMER_LEFT_8_TM=58.570
PRIMER_RIGHT_8_TM=60.008
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=889
PRIMER_PAIR_9_PENALTY=1.438124
PRIMER_LEFT_9_PENALTY=1.429867
PRIMER_RIGHT_9_PENALTY=0.008257
PRIMER_LEFT_9_SEQUENCE=CATGAGATTGTTGTGGCCTT
PRIMER_RIGHT_9_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_9=890,20
PRIMER_RIGHT_9=1779,20
PRIMER_LEFT_9_TM=58.570
PRIMER_RIGHT_9_TM=60.008
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=890
PRIMER_PAIR_10_PENALTY=1.438124
PRIMER_LEFT_10_PENALTY=1.429867
PRIMER_RIGHT_10_PENALTY=0.008257
PRIMER_LEFT_10_SEQUENCE=AAGGCATGAGATTGTTGTGG
PRIMER_RIGHT_10_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_10=886,20
PRIMER_RIGHT_10=1779,20
PRIMER_LEFT_10_TM=58.570
PRIMER_RIGHT_10_TM=60.008
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=894
PRIMER_PAIR_11_PENALTY=1.540165
PRIMER_LEFT_11_PENALTY=1.531908
PRIMER_RIGHT_11_PENALTY=0.008257
PRIMER_LEFT_11_SEQUENCE=ATGAGATTGTTGTGGCCTTGT
PRIMER_RIGHT_11_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_11=891,21
PRIMER_RIGHT_11=1779,20
PRIMER_LEFT_11_TM=59.468
PRIMER_RIGHT_11_TM=60.008
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=889
PRIMER_PAIR_12_PENALTY=1.823907
PRIMER_LEFT_12_PENALTY=1.815650
PRIMER_RIGHT_12_PENALTY=0.008257
PRIMER_LEFT_12_SEQUENCE=TGTTGTGGCCTTGTGAGATAA
PRIMER_RIGHT_12_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_12=898,21
PRIMER_RIGHT_12=1779,20
PRIMER_LEFT_12_TM=59.184
PRIMER_RIGHT_12_TM=60.008
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=882
PRIMER_PAIR_13_PENALTY=2.099671
PRIMER_LEFT_13_PENALTY=2.091414
PRIMER_RIGHT_13_PENALTY=0.008257
PRIMER_LEFT_13_SEQUENCE=GATTGTTGTGGCCTTGTGAGA
PRIMER_RIGHT_13_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_13=895,21
PRIMER_RIGHT_13=1779,20
PRIMER_LEFT_13_TM=61.091
PRIMER_RIGHT_13_TM=60.008
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=885
PRIMER_PAIR_14_PENALTY=2.099671
PRIMER_LEFT_14_PENALTY=2.091414
PRIMER_RIGHT_14_PENALTY=0.008257
PRIMER_LEFT_14_SEQUENCE=GAGATTGTTGTGGCCTTGTGA
PRIMER_RIGHT_14_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_14=893,21
PRIMER_RIGHT_14=1779,20
PRIMER_LEFT_14_TM=61.091
PRIMER_RIGHT_14_TM=60.008
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=887
PRIMER_PAIR_15_PENALTY=2.485781
PRIMER_LEFT_15_PENALTY=2.477524
PRIMER_RIGHT_15_PENALTY=0.008257
PRIMER_LEFT_15_SEQUENCE=CATGAGATTGTTGTGGCCTTG
PRIMER_RIGHT_15_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_15=890,21
PRIMER_RIGHT_15=1779,20
PRIMER_LEFT_15_TM=61.478
PRIMER_RIGHT_15_TM=60.008
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=890
PRIMER_PAIR_16_PENALTY=2.498898
PRIMER_LEFT_16_PENALTY=2.490641
PRIMER_RIGHT_16_PENALTY=0.008257
PRIMER_LEFT_16_SEQUENCE=ATGTGGTCGCAACAATCTGAA
PRIMER_RIGHT_16_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_16=854,21
PRIMER_RIGHT_16=1779,20
PRIMER_LEFT_16_TM=61.491
PRIMER_RIGHT_16_TM=60.008
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=926
PRIMER_PAIR_17_PENALTY=2.542509
PRIMER_LEFT_17_PENALTY=2.534252
PRIMER_RIGHT_17_PENALTY=0.008257
PRIMER_LEFT_17_SEQUENCE=TTGTTGTGGCCTTGTGAGATAA
PRIMER_RIGHT_17_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_17=897,22
PRIMER_RIGHT_17=1779,20
PRIMER_LEFT_17_TM=60.534
PRIMER_RIGHT_17_TM=60.008
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=5.9000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=883
PRIMER_PAIR_18_PENALTY=2.990631
PRIMER_LEFT_18_PENALTY=2.982374
PRIMER_RIGHT_18_PENALTY=0.008257
PRIMER_LEFT_18_SEQUENCE=AGTCACATTTTGTTCCGGTT
PRIMER_RIGHT_18_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_18=918,20
PRIMER_RIGHT_18=1779,20
PRIMER_LEFT_18_TM=57.018
PRIMER_RIGHT_18_TM=60.008
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=862
PRIMER_PAIR_19_PENALTY=3.082661
PRIMER_LEFT_19_PENALTY=3.074404
PRIMER_RIGHT_19_PENALTY=0.008257
PRIMER_LEFT_19_SEQUENCE=TGAGATTGTTGTGGCCTTGTG
PRIMER_RIGHT_19_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_19=892,21
PRIMER_RIGHT_19=1779,20
PRIMER_LEFT_19_TM=62.074
PRIMER_RIGHT_19_TM=60.008
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=888
PRIMER_PAIR_20_PENALTY=3.125151
PRIMER_LEFT_20_PENALTY=0.861164
PRIMER_RIGHT_20_PENALTY=2.263987
PRIMER_LEFT_20_SEQUENCE=ATTGTTGTGGCCTTGTGAGA
PRIMER_RIGHT_20_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_20=896,20
PRIMER_RIGHT_20=1780,21
PRIMER_LEFT_20_TM=59.139
PRIMER_RIGHT_20_TM=61.264
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=885
PRIMER_PAIR_21_PENALTY=3.125151
PRIMER_LEFT_21_PENALTY=0.861164
PRIMER_RIGHT_21_PENALTY=2.263987
PRIMER_LEFT_21_SEQUENCE=GATTGTTGTGGCCTTGTGAG
PRIMER_RIGHT_21_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_21=895,20
PRIMER_RIGHT_21=1780,21
PRIMER_LEFT_21_TM=59.139
PRIMER_RIGHT_21_TM=61.264
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=886
PRIMER_PAIR_22_PENALTY=3.125151
PRIMER_LEFT_22_PENALTY=0.861164
PRIMER_RIGHT_22_PENALTY=2.263987
PRIMER_LEFT_22_SEQUENCE=AGATTGTTGTGGCCTTGTGA
PRIMER_RIGHT_22_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_22=894,20
PRIMER_RIGHT_22=1780,21
PRIMER_LEFT_22_TM=59.139
PRIMER_RIGHT_22_TM=61.264
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=887
PRIMER_PAIR_23_PENALTY=3.125151
PRIMER_LEFT_23_PENALTY=0.861164
PRIMER_RIGHT_23_PENALTY=2.263987
PRIMER_LEFT_23_SEQUENCE=GAGATTGTTGTGGCCTTGTG
PRIMER_RIGHT_23_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_23=893,20
PRIMER_RIGHT_23=1780,21
PRIMER_LEFT_23_TM=59.139
PRIMER_RIGHT_23_TM=61.264
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=888
PRIMER_PAIR_24_PENALTY=3.125151
PRIMER_LEFT_24_PENALTY=0.861164
PRIMER_RIGHT_24_PENALTY=2.263987
PRIMER_LEFT_24_SEQUENCE=TGAGATTGTTGTGGCCTTGT
PRIMER_RIGHT_24_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_24=892,20
PRIMER_RIGHT_24=1780,21
PRIMER_LEFT_24_TM=59.139
PRIMER_RIGHT_24_TM=61.264
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=889
PRIMER_PAIR_25_PENALTY=3.261963
PRIMER_LEFT_25_PENALTY=3.253706
PRIMER_RIGHT_25_PENALTY=0.008257
PRIMER_LEFT_25_SEQUENCE=AAAAGGCATGAGATTGTTGTGG
PRIMER_RIGHT_25_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_25=884,22
PRIMER_RIGHT_25=1779,20
PRIMER_LEFT_25_TM=61.254
PRIMER_RIGHT_25_TM=60.008
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=896
PRIMER_PAIR_26_PENALTY=3.278605
PRIMER_LEFT_26_PENALTY=1.014618
PRIMER_RIGHT_26_PENALTY=2.263987
PRIMER_LEFT_26_SEQUENCE=AAAGGCATGAGATTGTTGTGG
PRIMER_RIGHT_26_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_26=885,21
PRIMER_RIGHT_26=1780,21
PRIMER_LEFT_26_TM=59.985
PRIMER_RIGHT_26_TM=61.264
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=896
PRIMER_PAIR_27_PENALTY=3.356641
PRIMER_LEFT_27_PENALTY=3.348384
PRIMER_RIGHT_27_PENALTY=0.008257
PRIMER_LEFT_27_SEQUENCE=GCATGAGATTGTTGTGGCCTT
PRIMER_RIGHT_27_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_27=889,21
PRIMER_RIGHT_27=1779,20
PRIMER_LEFT_27_TM=62.348
PRIMER_RIGHT_27_TM=60.008
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=891
PRIMER_PAIR_28_PENALTY=3.427590
PRIMER_LEFT_28_PENALTY=1.163603
PRIMER_RIGHT_28_PENALTY=2.263987
PRIMER_LEFT_28_SEQUENCE=AGATTGTTGTGGCCTTGTGAG
PRIMER_RIGHT_28_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_28=894,21
PRIMER_RIGHT_28=1780,21
PRIMER_LEFT_28_TM=60.164
PRIMER_RIGHT_28_TM=61.264
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=887
PRIMER_PAIR_29_PENALTY=3.452752
PRIMER_LEFT_29_PENALTY=3.444495
PRIMER_RIGHT_29_PENALTY=0.008257
PRIMER_LEFT_29_SEQUENCE=TATGTGGTCGCAACAATCTGAA
PRIMER_RIGHT_29_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_29=853,22
PRIMER_RIGHT_29=1779,20
PRIMER_LEFT_29_TM=61.444
PRIMER_RIGHT_29_TM=60.008
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=927
PRIMER_PAIR_30_PENALTY=3.540719
PRIMER_LEFT_30_PENALTY=1.276733
PRIMER_RIGHT_30_PENALTY=2.263987
PRIMER_LEFT_30_SEQUENCE=TGTGGTCGCAACAATCTGAA
PRIMER_RIGHT_30_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_30=855,20
PRIMER_RIGHT_30=1780,21
PRIMER_LEFT_30_TM=61.277
PRIMER_RIGHT_30_TM=61.264
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=926
PRIMER_PAIR_31_PENALTY=3.664256
PRIMER_LEFT_31_PENALTY=3.655999
PRIMER_RIGHT_31_PENALTY=0.008257
PRIMER_LEFT_31_SEQUENCE=TTGTGGCCTTGTGAGATAAGT
PRIMER_RIGHT_31_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_31=900,21
PRIMER_RIGHT_31=1779,20
PRIMER_LEFT_31_TM=57.344
PRIMER_RIGHT_31_TM=60.008
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=5.7000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=880
PRIMER_PAIR_32_PENALTY=3.693854
PRIMER_LEFT_32_PENALTY=1.429867
PRIMER_RIGHT_32_PENALTY=2.263987
PRIMER_LEFT_32_SEQUENCE=ATGAGATTGTTGTGGCCTTG
PRIMER_RIGHT_32_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_32=891,20
PRIMER_RIGHT_32=1780,21
PRIMER_LEFT_32_TM=58.570
PRIMER_RIGHT_32_TM=61.264
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.5000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=890
PRIMER_PAIR_33_PENALTY=3.693854
PRIMER_LEFT_33_PENALTY=1.429867
PRIMER_RIGHT_33_PENALTY=2.263987
PRIMER_LEFT_33_SEQUENCE=CATGAGATTGTTGTGGCCTT
PRIMER_RIGHT_33_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_33=890,20
PRIMER_RIGHT_33=1780,21
PRIMER_LEFT_33_TM=58.570
PRIMER_RIGHT_33_TM=61.264
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=891
PRIMER_PAIR_34_PENALTY=3.693854
PRIMER_LEFT_34_PENALTY=1.429867
PRIMER_RIGHT_34_PENALTY=2.263987
PRIMER_LEFT_34_SEQUENCE=AAGGCATGAGATTGTTGTGG
PRIMER_RIGHT_34_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_34=886,20
PRIMER_RIGHT_34=1780,21
PRIMER_LEFT_34_TM=58.570
PRIMER_RIGHT_34_TM=61.264
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=895
PRIMER_PAIR_35_PENALTY=3.755472
PRIMER_LEFT_35_PENALTY=3.747215
PRIMER_RIGHT_35_PENALTY=0.008257
PRIMER_LEFT_35_SEQUENCE=GTTGTGGCCTTGTGAGATAAGT
PRIMER_RIGHT_35_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_35=899,22
PRIMER_RIGHT_35=1779,20
PRIMER_LEFT_35_TM=58.253
PRIMER_RIGHT_35_TM=60.008
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=5.7000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=881
PRIMER_PAIR_36_PENALTY=3.795895
PRIMER_LEFT_36_PENALTY=1.531908
PRIMER_RIGHT_36_PENALTY=2.263987
PRIMER_LEFT_36_SEQUENCE=ATGAGATTGTTGTGGCCTTGT
PRIMER_RIGHT_36_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_36=891,21
PRIMER_RIGHT_36=1780,21
PRIMER_LEFT_36_TM=59.468
PRIMER_RIGHT_36_TM=61.264
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=890
PRIMER_PAIR_37_PENALTY=3.821500
PRIMER_LEFT_37_PENALTY=3.813243
PRIMER_RIGHT_37_PENALTY=0.008257
PRIMER_LEFT_37_SEQUENCE=GTTGTGGCCTTGTGAGATAA
PRIMER_RIGHT_37_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_37=899,20
PRIMER_RIGHT_37=1779,20
PRIMER_LEFT_37_TM=56.187
PRIMER_RIGHT_37_TM=60.008
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.9000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=881
PRIMER_PAIR_38_PENALTY=3.821500
PRIMER_LEFT_38_PENALTY=3.813243
PRIMER_RIGHT_38_PENALTY=0.008257
PRIMER_LEFT_38_SEQUENCE=AAAGGCATGAGATTGTTGTG
PRIMER_RIGHT_38_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_38=885,20
PRIMER_RIGHT_38=1779,20
PRIMER_LEFT_38_TM=56.187
PRIMER_RIGHT_38_TM=60.008
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=895
PRIMER_PAIR_39_PENALTY=3.848587
PRIMER_LEFT_39_PENALTY=0.861164
PRIMER_RIGHT_39_PENALTY=2.987423
PRIMER_LEFT_39_SEQUENCE=ATTGTTGTGGCCTTGTGAGA
PRIMER_RIGHT_39_SEQUENCE=TCTATATGTAGGCTTCGCTCCA
PRIMER_LEFT_39=896,20
PRIMER_RIGHT_39=1826,22
PRIMER_LEFT_39_TM=59.139
PRIMER_RIGHT_39_TM=59.013
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=931
PRIMER_PAIR_40_PENALTY=3.848587
PRIMER_LEFT_40_PENALTY=0.861164
PRIMER_RIGHT_40_PENALTY=2.987423
PRIMER_LEFT_40_SEQUENCE=GATTGTTGTGGCCTTGTGAG
PRIMER_RIGHT_40_SEQUENCE=TCTATATGTAGGCTTCGCTCCA
PRIMER_LEFT_40=895,20
PRIMER_RIGHT_40=1826,22
PRIMER_LEFT_40_TM=59.139
PRIMER_RIGHT_40_TM=59.013
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.455
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=932
PRIMER_PAIR_41_PENALTY=3.848587
PRIMER_LEFT_41_PENALTY=0.861164
PRIMER_RIGHT_41_PENALTY=2.987423
PRIMER_LEFT_41_SEQUENCE=AGATTGTTGTGGCCTTGTGA
PRIMER_RIGHT_41_SEQUENCE=TCTATATGTAGGCTTCGCTCCA
PRIMER_LEFT_41=894,20
PRIMER_RIGHT_41=1826,22
PRIMER_LEFT_41_TM=59.139
PRIMER_RIGHT_41_TM=59.013
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=933
PRIMER_PAIR_42_PENALTY=3.848587
PRIMER_LEFT_42_PENALTY=0.861164
PRIMER_RIGHT_42_PENALTY=2.987423
PRIMER_LEFT_42_SEQUENCE=GAGATTGTTGTGGCCTTGTG
PRIMER_RIGHT_42_SEQUENCE=TCTATATGTAGGCTTCGCTCCA
PRIMER_LEFT_42=893,20
PRIMER_RIGHT_42=1826,22
PRIMER_LEFT_42_TM=59.139
PRIMER_RIGHT_42_TM=59.013
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=934
PRIMER_PAIR_43_PENALTY=3.848587
PRIMER_LEFT_43_PENALTY=0.861164
PRIMER_RIGHT_43_PENALTY=2.987423
PRIMER_LEFT_43_SEQUENCE=TGAGATTGTTGTGGCCTTGT
PRIMER_RIGHT_43_SEQUENCE=TCTATATGTAGGCTTCGCTCCA
PRIMER_LEFT_43=892,20
PRIMER_RIGHT_43=1826,22
PRIMER_LEFT_43_TM=59.139
PRIMER_RIGHT_43_TM=59.013
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=45.455
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=935
PRIMER_PAIR_44_PENALTY=3.954242
PRIMER_LEFT_44_PENALTY=3.945985
PRIMER_RIGHT_44_PENALTY=0.008257
PRIMER_LEFT_44_SEQUENCE=TGTTGTGGCCTTGTGAGATAAGT
PRIMER_RIGHT_44_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_44=898,23
PRIMER_RIGHT_44=1779,20
PRIMER_LEFT_44_TM=60.946
PRIMER_RIGHT_44_TM=60.008
PRIMER_LEFT_44_GC_PERCENT=43.478
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=5.7000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=882
PRIMER_PAIR_45_PENALTY=4.001269
PRIMER_LEFT_45_PENALTY=3.993012
PRIMER_RIGHT_45_PENALTY=0.008257
PRIMER_LEFT_45_SEQUENCE=AGATTGTTGTGGCCTTGTGAGA
PRIMER_RIGHT_45_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_45=894,22
PRIMER_RIGHT_45=1779,20
PRIMER_LEFT_45_TM=61.993
PRIMER_RIGHT_45_TM=60.008
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=886
PRIMER_PAIR_46_PENALTY=4.001269
PRIMER_LEFT_46_PENALTY=3.993012
PRIMER_RIGHT_46_PENALTY=0.008257
PRIMER_LEFT_46_SEQUENCE=GAGATTGTTGTGGCCTTGTGAG
PRIMER_RIGHT_46_SEQUENCE=AACACCCAAAAACAGCTTGG
PRIMER_LEFT_46=893,22
PRIMER_RIGHT_46=1779,20
PRIMER_LEFT_46_TM=61.993
PRIMER_RIGHT_46_TM=60.008
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=887
PRIMER_PAIR_47_PENALTY=4.002042
PRIMER_LEFT_47_PENALTY=1.014618
PRIMER_RIGHT_47_PENALTY=2.987423
PRIMER_LEFT_47_SEQUENCE=AAAGGCATGAGATTGTTGTGG
PRIMER_RIGHT_47_SEQUENCE=TCTATATGTAGGCTTCGCTCCA
PRIMER_LEFT_47=885,21
PRIMER_RIGHT_47=1826,22
PRIMER_LEFT_47_TM=59.985
PRIMER_RIGHT_47_TM=59.013
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=942
PRIMER_PAIR_48_PENALTY=4.079637
PRIMER_LEFT_48_PENALTY=1.815650
PRIMER_RIGHT_48_PENALTY=2.263987
PRIMER_LEFT_48_SEQUENCE=TGTTGTGGCCTTGTGAGATAA
PRIMER_RIGHT_48_SEQUENCE=AAACACCCAAAAACAGCTTGG
PRIMER_LEFT_48=898,21
PRIMER_RIGHT_48=1780,21
PRIMER_LEFT_48_TM=59.184
PRIMER_RIGHT_48_TM=61.264
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=5.9000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=883
PRIMER_PAIR_49_PENALTY=4.090841
PRIMER_LEFT_49_PENALTY=0.861164
PRIMER_RIGHT_49_PENALTY=3.229677
PRIMER_LEFT_49_SEQUENCE=ATTGTTGTGGCCTTGTGAGA
PRIMER_RIGHT_49_SEQUENCE=TAAACACCCAAAAACAGCTTGG
PRIMER_LEFT_49=896,20
PRIMER_RIGHT_49=1781,22
PRIMER_LEFT_49_TM=59.139
PRIMER_RIGHT_49_TM=61.230
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=886
=
SEQUENCE_ID=MAGIC.105_2_18093609_18093692_oy-0_hi-0_4178_962440_TRUE
SEQUENCE_TARGET=950,183
SEQUENCE_TEMPLATE=GTATCATCATTGGCTTGATAGCATGAAGAAGAGGGAAATCGGCAGTTATTAAAGGTNGTGGTGGTGGTGAAAGAGCTAATTCTTGGACNNNGAGTGGTGTTGCGGCAACATGAGATTTTTGTTNNTTTTTTTGANTAGATTTTTATGATATTATGGGTTTGTGNTTNTTCAGCTATGAGATTATTATGAGATTATGGGTTTNTAAATTTAAAAGCGTNNTTCATAATTGGTATTAGAAAAGACGCTCATGAATCTGAANTATTTAAGANANNNNNNNNNNNNNNNNNNNATNAAANCTTAATCATNAGNNNNNNNNNNNNNNNAAGNAAGTGGAATTCCAATTTTGNNNNNNNNNNACAAAACCCNNANNNTNAAATNGGGTGAAGNAGNNNNNNNNGANAGGAAANNNGAAGAAGGNNATTNGNTTTGNNTTTAATNNGGTNNAAATNAGGTTTTNTTNNNNNNNNNNNGGTTAANTAAANAATAANCGNNNAAANNCANANATNNGGNTNAATAGATNTCGNANTATTAATTCGNNGAGATTTAGCTATNGAACTTANGTTTNNGGGAGAAATANNNNTANAANNTTGNTTTNNNNGANATNTNGACCNNNGTTNAAGTTTGGNNTCTATCTNNNATGATGNNNTACTTNTCGTNNGNAANNANACCGTTTNTCNNNNNNTATCCTGATCCNTNTNTATTGAAAAAAGTTTGAATTACTTTTCCTTCATTTTTTAAACTGTCAATATCTTATTTCATTCAAATATGCATACATTTTTTAGGCCTATTTATCGATCGNTCTTAGTTATGGAAATTTTACNNCNNNNNTCATCATACNAATNTACATTACTCGGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACCATCTGGTTACATTTTTAATNATTATATATCATCNTTACAANTGTCNGACNAAATGGTGAATNGAGAATCANTAGANGGAACCACTNAGACAANTAATNTTTTNNANAATTTACNATTTGTTCACACTTTTTCTTTCNTTCNTAAAGAATTTTCTCTNAAGTTTNNGCTTATGTAATTTGNTNNNANGGAAAAAATTNAAANNNNNNNNNNTGTAAAATTNGATAACCATTAANCANANAAAANGTTGAANTTTAGTAAATGCGTTTATTATGGATTGGNNNNNNNNCTGATATACTTCANTCTNTGACAACCGATNCAAANAACACATTGGTNCATTAGNATACACCGCATAATCNTTTTTATTCGNCACCTTTAGTTATAAACTCACACNTGNACGNCCACACTTATACTATGAAACCGTGTCATNCGNACCACATTTTAANNNNNNNNNCAACTAAGATTTGCTCCAGGAGTGATCCCAAGCGTCCTGCAGAACTCCAAGTAATAGTTTACTCTGCTCTGCACGGCTGCAGGACGCCTACCGTTGCATTCACCACCATTGATCTTCCTAGTGGTGGCTCCAAAGCCTTGGTCCAAGACTGGACGAACATTTAGGTTCCAGAACCACATGGCACACTGAAAGGCAACTTGGGGGCTACGAGCCACCATGTCTGGATCAGTCAAGAGAGGGAGTCCGAGGAACTTTCCAGCTGCACCGTANTTGTAGTTCCATGTGATTTGGATTGGACCGCGGCCGTANTAGTCTTTTCCCGGTGTACAAGGGTACGCNGTGCTCGGTGAGCAGTATTTCCCTCTAGCTATTTCTTCTTTGTAGCAAAAACCTTGCATAACGTAAAAGANNATCGTTATTTANAATAGAGAATGAATATGTATGAATTTTAATGATAAAAGAAATGATTTGTTAACTTACCCAAGAAACATTATGGTGCTTAATTAGTGTAATAATATTCTTGATAATAATAATTCATTAATTTNCTTAATTAAATAATTAGTNTACATGCATTCATGTAGTAGAACTAGAAGGGTTTATAAAGAACTTACTTCCAGATTCATGAGAAAACTGAGCCAACATAGCGGCAATCTCACGCTTAGCGACGGTTCCTTTATAGGCGTCGAACGATTGGGCAGCCGCAATGAAAGCCTGNCGAGTGTAGAACCCTTTCG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_7293478_7293535_po-0_zu-0_93910_59760_TRUE
SEQUENCE_TARGET=950,157
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGGATATTTCTCTTAGTTAGTTTGAAGGTTNACNTTATTTGTAATTTAATGCTTTTGATAATTGNAAGGGTCGTGGGCTCGTACATTTCCTCGAGCTATTTGTATTTTNCTAGCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_1641738_1641907_bur-0_edi-0_1440_1361444_TRUE
SEQUENCE_TARGET=950,269
SEQUENCE_TEMPLATE=ACACACTCAANCACATCACATNTNAAGAAGCACCCGATTACGCCGACAANAAGTGTCTTCTTTGCGAGGAGGAGTTNGATCNACAACATCCCAAAATTCACCATTGTGATGTTTGTAATTTCANCATATGTAGANCATGTATGAAAANCGCACCACCNGTTCGTNTTGAAAGCCNTACNACCCATGAACATCANCTNCATCTCGTTCCAAGACGCATTGATTTCACTTGCAATGCTTGNGGGACGCTAGGTGACCGAAGTCCTTANTTNTGNCNNNNNNNNNNNNNNNNNNTTCATCGNCAGTGTATCGATTTACCNCGNGTCATAAACATCAACCGCCACNATCACCGCATCTCTTACACCCGTCGCCTTGGACATGGAGANTGGAAATGNAAANTCTGCCGCAAAAAAGTNGATGGGTTCTANGGGGCTTATACTTGCCTCAAATGTCCTAGTTTTGTTGTTCATTCACTATGNGCAACAAGAAANGATGTGTGGGACATGATAGAGCTAGAAGGGATGCNNGAAGAAGAGGAAATTGCNCCATACGAGATGATTGNTGCTAACACGATAAAACATTTCAGCCATGATCATAACNTACAAATCAACAATGATGGNCATNGCNGNATTCAACCTGAAAATATAGTTTGTGAAGCNTGTGTNTTCCAGATNTTNTCAGAACCGTTCTACAGTTGCAACCAATGTAGTTTTATNCTCCACNAAAAATGTGCAAANCATCCACGGAAGAAACGNCACNTGNGCAACAACANGNCNTTCATANTNTTCACAGATTCTTNCAACAAAGNTNANGCTTCGTGTGNTCTTTGTAAACAACANTTCACTGGNTTCAGATANNAANCAGGCCNTTATNTTNCTNTAGATGTACGATGCGCNGCAATTTTGGATACANTTGAGCANGGAAGCCATCAACATCCTTTATATTACTACNNTACATNTGNCTGGAAGCCTTGTANNNNNNNNNNNNNNNNNNNNGNCNTTTTNTTNCGTTGTGATGAGTGTGACTANATTTTGGATGGAAANTGTGCTNTTNTTCCNAAAAAGGTAATGAGAAATAGNTACGATGATCATCTTCTNTNNTTGTCTTTCGGTGACNAGAATGTGAGTGGTGACTATTGGTGTGAAGCATGTGAAACNAAAGTGAACCCAAAGNAATGGTTCTATACNTGCAANGATTGTGGAACCATATTACATATATCTTGTNTTGTGGGAGATTTCTCATATATCATGCCAGGTTCTCATATAANAACATGGAGAGCTANNGTGGTATCCAACACCTCTATTTGTAGACNAGTTTGNTATATGTGTAACTCTCGATGCAANCTTCCNNNNGTTCTTAAGCTTTNTGACACCNAAGTTGATGTATACNTTTGTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13172732_13172987_wu-0_can-0_13069_31349_TRUE
SEQUENCE_TARGET=950,355
SEQUENCE_TEMPLATE=NTNGGGAGAGTTGGAGNAATGAAAGCTNNNAANTNNAAATNATAAAATNNATTANNNNNNNNNNNNNNNNNNNCNAAAAGNCATATNTNACCTAANTTCNACCAGGTTTNNNAGACCACTTCGATGGAAGCANNNNNNNNNNNTTTCNAATCGAGGCAGGGGTTTTNTTAATCANNAACCAAGGATGGNGCCAANTCTCATCCTTGTTCGNNANATTCGNGATGANATTCNTGTTGGNGTTNGGNTACNCTGAAAANTACCNNGTTTTNTTCGNCGAACTCCTNANACTGAGAAGTTNGTTCAATTCNGGAACCCCGATGANANAANCGNNNNNNGNNNCGATGGTAATAATACNCANGATGGTTNGGAGGAGGTTANGGGTNANTTGAGGTANGGNGATTTCNAGNGCNGCCAAATATCNAACNANNGCTTNGGGAAGAGGNAAGGTCAATCNGCANCNTTTGAAGTAGATTNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTCCNCNGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGATTGAAGAGTNCANGNGGNTTGNTAATGTNNNCCGGCNATATCNTTNNGNCGGGTGNANATGTCATTNCNAGAACCACCNTCNTNGAAAGATGGAGATTCNNGCCTTTNNTNNNCANAANNNNNNNTAAGANAATGAACAGNCTNGGGGTTNGAGGGAGAGAAAGTGNAGAAAGGAANAAGNANNNNNNNNNNNNNNNNNNNNNNNNNNNAGCGTGNAANATANTAAGGAGNNAGTNAAGTNAATNTACCTTACGCAAGCNAGTNANGATGNNGGAACCGCCTTGGNAGTNNTGGGGGAAGTGGAAGAGNTTTNNCCGCTTNAATAGTAANTANTTGNTATNCGGTCATAANTGTGACAGATGTCACGAGAGTAANTATGNGTNNTTCNGAAATCANAGNANTNACGTNNTTTTGGNGNGAAATNAGAGANTCACTTNGCGNAATCTTTGNCCNTTAGAGCATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAANATTNCCANGTAAGNAGGAGAGGCCNAANAGAGGAAANGCCCACNGGNTTCCTTGAGCAAGNATATANNNNNNNNNNNNNNNNNNNGAGNTGAANGTGACATCNAANTCNAAGCNANCCNNNNNNNNNNNNNNNNNNNNNNGNAAGGNGAAGCCNAGGAAGTCCGGANTCAATNCAAATTNNAACGTNTGAANAGTTAATTCGAAGANNGAAACNGATACATTTAAGATNGAATAATTGNGTGTAAATGAGAANTAAATGTATTCTNTGAGTATAAATTNTGNGTCAAGGACCATGNAAANANACACNCANTTTTTGTGCAAANNNACNATACACATTTNCTTACAAAACTCATAGTTTTCTCTATAATCATCAATCTATTTTCTAGTANATTTGTCCTCAAGAATAATCCTTCTCACTAAATAAATAAATACTTGAGTGTGTNAATNNGACATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_18805742_18805789_sf-2_ws-0_991867_198701_TRUE
SEQUENCE_TARGET=950,147
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGANANTTNTANGNTAATTAATTNAAAAATTAAGAANTTAGAGTAATNTNNGAATTNGATAGNTTTCGTGNTAGGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_8561977_8562148_wil-2_ct-1_48286_4975_TRUE
SEQUENCE_TARGET=950,271
SEQUENCE_TEMPLATE=TAACNNGTTTGACATATATATTTCAAAANCCACTAACNAAAATATTAATAAATACTAGTTTTTCGAAGNNAANAAAAAAACGTTTGCATTATTTAGTCTTGAAACAACNAATTNAAAAGGAAAGGTTGTCGGTGAACAAATTTGAAGGCCACTNGAATAATGCCTTNTATTTTTGTCAGAAGNTTACATTGNCTAGGTACTTGTTTAACTTAATTCNTACAATTTCTGATACCAATTGTGTAATATCACTGAGTTAGTTGTTNNTGTTGTTTCATTANCCAAAAAACAAAAGTTCTACACGTTGGCATNCCTTTCTTTTGGTCATATATGAGTTTGATAACAATATATAAACTTCAAAATCAGGATTTTCTTTGCTAAATCGAAAGAGTTTCGTANAANATTATTGATTGTTTTAAGTTTAATTAAGTAACTTGTAGGTTTAATTCAAAAACTTGTAGGTATCTCNGTGATATTANTGGTTTATGAAANNNNNTATTAANGCTTGTNTCGANTTTGTNTTNGGCCGGCCTTTCTTANCAGAATTGAACCTCAGANAGAAACTGTAATAAACCAAAGATACTGGACATGTNTATGTCGGCCTTTGGNGTCGTCTGTAACTAAAAANAAACTGGATANTTATCCNNGATATTACTAATCNTNTGAAATCTTNTTGTAATTATCTATAGATTNTTAGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTTGAATGTAAAATCCANNNTTGGATAGTATCCAATCNGTAGCCGNTATNNTCCCAACTTTTAATCTNAAATATGCATGATTANAANTATCAAAATATGATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_14499539_14499542_edi-0_sf-2_172797_102485_TRUE
SEQUENCE_TARGET=950,103
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACTAAACAAGNTTCNTCTTGCTTCTCAAAGCTTTGATGGTTTAGCCAAAGTCTGTATGAGTCTTTGGTTNNGTATCTTCTAACAAGGAAACACTACTTAGGCTTTAAAGATCTGAATGTTGTTCTTGTTTTTATACTCAATCATACACATGACANANATATTTGACTNNNTAACATTAAACAAGATTCATCTTGCTTCTCAAAGCTTTGATGGTGTAGCCGAATTCCATATGAGTATTTGNTTTGTATCTTATAACAAGAAAACACTACTTAGGCTTTTAAGGTCGAGTTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_12828088_12828266_rsch-4_sf-2_108514_107983_TRUE
SEQUENCE_TARGET=950,278
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCCTTAGNTTTGAGTCGTTTGTCGTGTCATAGTCCGTCTTTTGCGTCTTTTCGGCTGTGTCTTGAGTCNTTTGCANNTTTTAGGNGTTTAAGCGNGACGCNGATGCTTTCNGGACNTATCAGGCTCATTTGGACCAGNAANGNCNNTGGNAATCGAGAAACCGGGATCATGAACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_14270157_14270243_hi-0_rsch-4_166624_4404_TRUE
SEQUENCE_TARGET=950,186
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAGTTTANATGTNCAAAAAGTGNTTTTTGNNGCCANTACAATGGANATAATCAAAGCCCTNNATAAACCNAAGGANTNNCCATCAATNNTAAGTCACANTNTCNNNNNNNNNNNNNTGGCTGATGGAATGNNTGANTGGTTCATTTTATTTGAACCTAATGGGTNNAANAAAGGNGCCTATAAAATANCNAAGAGTGTTACCTNTNACANNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_13672567_13673299_ws-0_tsu-0_186687_218485_TRUE
SEQUENCE_TARGET=950,832
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCGTGATCTTTACTATATTACGGTGAGAAAATTATAGGTTCGGACATTGTTTTCNAATATTTTGCATGTATCAAAGCTCTTATAAACAAGATAGGATATTCCTACATAGCNGGTGGGACCNCAACAAATGGATCATGAAGTCTTCAAAAAAGAATAGATACGATTGCATTGCATGTAGTAACAGATGCGATCATACCAGCACTAATGCACCAGATACCATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTGCATAACTCTTTTGTTNTCGATTTTTCTCATNTTTTTTGTTAAAAATGTATTACTCTATAACTTTTAGACCGTGAGGCCAAACTTGGCATGTGATACCTTTTCGGAAAGCCNAAAGAGTGCTCTCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAATTTTGACCTAAATGTACTTTTNATGGCCTTTTCTTGATCTTTGCTATATTACGGGACAAAAATTATATGTTCGGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14772676_14772703_po-0_tsu-0_120991_5259_TRUE
SEQUENCE_TARGET=950,127
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACGTGTNTTGTTTGTATCTTTTAGCAAATCTCCCTAAATAANTTTTGCTTGTATCAGTANAATTAGTAGANNTCGTATTAGTCTTTCACTATTTTGTGGGAAAAAATATCTACGAGAAACTTGTGTTCGTANCTACTGTCTCACTATCGTTCTCAAGGACATCATNCCACAAGAGNNTAACNAAAAATATAAGATCTGAAAAAAATNNAAGGGTAANAAAACAAAACAAAANTAGACAACCTCNCCGGATNGCCTCNTCACCATCGCTGTTACAACGANAACATTAAGCAACNTAACNTTCTATATCAACAAGTTAAAGACANANCAGNATATGATNTCTTACCTAGTAGTTGAGGGAGTGAAGAATCTTCAAGTTTTACTCGCACTTGATTGAGCGGTAAATGTGACGAACAAAGAGAAGGGAAGCACNGAATCCGATTGTCCCGAGCATNAGGAAGAATCCGTAGCAGATGCAAGCCATGTAGCCAAAGAAGAAGGAAGTCTGCATGAACCCTGACATATCTGANCGTGCATAGTAGTAATACAAGCAGTAGGCATATATGAATAAGCCCGTTGATCCACCGCATAGCAATGATCTTTNCATGCAGAAGATANAAAAAAGTNAGATTAACAATTTATAGACANACTCAAACATGGGATTCTCTTNTAGGCTAAAGCATTGATGAATCGCAATTATGNAACNAACAACNGACAAGTATATATTCCCTGANCCAGATGAATAATGGAACCAGAAGTTAANGAAAGANAAANGAAAACATTACCTCCACCACCACTCATGGTCTTCAGNAGCAAGTTGGAAGTACGTTAATGCAACCGTGATNAAAGCAGTGACTATNACNAGAATAAGGAAGACGATAGACAGAATACTATAGATGGTGTATATTCTGTGACCCCAAACACTGGCAAATATGTANTAAAGCTCGATGTAAATGGCACTGAANGGTAAAAAACCAGCCATAGCCATCTGCGGAAGTGTTCTCCNGTACCAACGCATTGGTGGGATCTCTCTTGGATATTTTGTGGTACGACAAGGAGCTTGAAACTCNGATTTTCTGTTCTTCCCAGCAATACCTCCNAATATTAACAATGGTGAAGTAACNAGAGCCCATATAAGGAAAATAACCACAATTGTNCCAAATGGCAANGCNGCAGTGGCTTGGTAAGCAATCGCAACAGTATTAAGGAAGGAGAANGTGATCAAAAGAGGTCCACAGAAGAGGCT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.947351
PRIMER_LEFT_0_PENALTY=4.851580
PRIMER_RIGHT_0_PENALTY=0.095771
PRIMER_LEFT_0_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_0_SEQUENCE=CAGATGGCTATGGCTGGTTT
PRIMER_LEFT_0=919,23
PRIMER_RIGHT_0=1771,20
PRIMER_LEFT_0_TM=58.148
PRIMER_RIGHT_0_TM=60.096
PRIMER_LEFT_0_GC_PERCENT=47.826
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=853
PRIMER_PAIR_1_PENALTY=5.012976
PRIMER_LEFT_1_PENALTY=4.917205
PRIMER_RIGHT_1_PENALTY=0.095771
PRIMER_LEFT_1_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_1_SEQUENCE=CAGATGGCTATGGCTGGTTT
PRIMER_LEFT_1=920,22
PRIMER_RIGHT_1=1771,20
PRIMER_LEFT_1_TM=57.083
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=852
PRIMER_PAIR_2_PENALTY=5.078936
PRIMER_LEFT_2_PENALTY=4.851580
PRIMER_RIGHT_2_PENALTY=0.227356
PRIMER_LEFT_2_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_2_SEQUENCE=TATTTGCCAGTGTTTGGGGT
PRIMER_LEFT_2=919,23
PRIMER_RIGHT_2=1713,20
PRIMER_LEFT_2_TM=58.148
PRIMER_RIGHT_2_TM=60.227
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=10.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=795
PRIMER_PAIR_3_PENALTY=5.106332
PRIMER_LEFT_3_PENALTY=5.010561
PRIMER_RIGHT_3_PENALTY=0.095771
PRIMER_LEFT_3_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_3_SEQUENCE=CAGATGGCTATGGCTGGTTT
PRIMER_LEFT_3=918,24
PRIMER_RIGHT_3=1771,20
PRIMER_LEFT_3_TM=58.989
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.833
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=854
PRIMER_PAIR_4_PENALTY=5.144561
PRIMER_LEFT_4_PENALTY=4.917205
PRIMER_RIGHT_4_PENALTY=0.227356
PRIMER_LEFT_4_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_4_SEQUENCE=TATTTGCCAGTGTTTGGGGT
PRIMER_LEFT_4=920,22
PRIMER_RIGHT_4=1713,20
PRIMER_LEFT_4_TM=57.083
PRIMER_RIGHT_4_TM=60.227
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=10.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=794
PRIMER_PAIR_5_PENALTY=5.166550
PRIMER_LEFT_5_PENALTY=4.851580
PRIMER_RIGHT_5_PENALTY=0.314970
PRIMER_LEFT_5_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_5_SEQUENCE=ATATTTGCCAGTGTTTGGGG
PRIMER_LEFT_5=919,23
PRIMER_RIGHT_5=1714,20
PRIMER_LEFT_5_TM=58.148
PRIMER_RIGHT_5_TM=59.685
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=11.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=796
PRIMER_PAIR_6_PENALTY=5.232176
PRIMER_LEFT_6_PENALTY=4.917205
PRIMER_RIGHT_6_PENALTY=0.314970
PRIMER_LEFT_6_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_6_SEQUENCE=ATATTTGCCAGTGTTTGGGG
PRIMER_LEFT_6=920,22
PRIMER_RIGHT_6=1714,20
PRIMER_LEFT_6_TM=57.083
PRIMER_RIGHT_6_TM=59.685
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=11.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=795
PRIMER_PAIR_7_PENALTY=5.237917
PRIMER_LEFT_7_PENALTY=5.010561
PRIMER_RIGHT_7_PENALTY=0.227356
PRIMER_LEFT_7_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_7_SEQUENCE=TATTTGCCAGTGTTTGGGGT
PRIMER_LEFT_7=918,24
PRIMER_RIGHT_7=1713,20
PRIMER_LEFT_7_TM=58.989
PRIMER_RIGHT_7_TM=60.227
PRIMER_LEFT_7_GC_PERCENT=45.833
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=10.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=796
PRIMER_PAIR_8_PENALTY=5.325531
PRIMER_LEFT_8_PENALTY=5.010561
PRIMER_RIGHT_8_PENALTY=0.314970
PRIMER_LEFT_8_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_8_SEQUENCE=ATATTTGCCAGTGTTTGGGG
PRIMER_LEFT_8=918,24
PRIMER_RIGHT_8=1714,20
PRIMER_LEFT_8_TM=58.989
PRIMER_RIGHT_8_TM=59.685
PRIMER_LEFT_8_GC_PERCENT=45.833
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=11.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=797
PRIMER_PAIR_9_PENALTY=5.391192
PRIMER_LEFT_9_PENALTY=4.851580
PRIMER_RIGHT_9_PENALTY=0.539612
PRIMER_LEFT_9_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_9_SEQUENCE=ATCCAAGAGAGATCCCACCA
PRIMER_LEFT_9=919,23
PRIMER_RIGHT_9=1818,20
PRIMER_LEFT_9_TM=58.148
PRIMER_RIGHT_9_TM=59.460
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=900
PRIMER_PAIR_10_PENALTY=5.441895
PRIMER_LEFT_10_PENALTY=4.851580
PRIMER_RIGHT_10_PENALTY=0.590315
PRIMER_LEFT_10_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_10_SEQUENCE=TCCAAGAGAGATCCCACCAA
PRIMER_LEFT_10=919,23
PRIMER_RIGHT_10=1817,20
PRIMER_LEFT_10_TM=58.148
PRIMER_RIGHT_10_TM=60.590
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=899
PRIMER_PAIR_11_PENALTY=5.456818
PRIMER_LEFT_11_PENALTY=4.917205
PRIMER_RIGHT_11_PENALTY=0.539612
PRIMER_LEFT_11_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_11_SEQUENCE=ATCCAAGAGAGATCCCACCA
PRIMER_LEFT_11=920,22
PRIMER_RIGHT_11=1818,20
PRIMER_LEFT_11_TM=57.083
PRIMER_RIGHT_11_TM=59.460
PRIMER_LEFT_11_GC_PERCENT=45.455
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=899
PRIMER_PAIR_12_PENALTY=5.507520
PRIMER_LEFT_12_PENALTY=4.917205
PRIMER_RIGHT_12_PENALTY=0.590315
PRIMER_LEFT_12_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_12_SEQUENCE=TCCAAGAGAGATCCCACCAA
PRIMER_LEFT_12=920,22
PRIMER_RIGHT_12=1817,20
PRIMER_LEFT_12_TM=57.083
PRIMER_RIGHT_12_TM=60.590
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=898
PRIMER_PAIR_13_PENALTY=5.550173
PRIMER_LEFT_13_PENALTY=5.010561
PRIMER_RIGHT_13_PENALTY=0.539612
PRIMER_LEFT_13_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_13_SEQUENCE=ATCCAAGAGAGATCCCACCA
PRIMER_LEFT_13=918,24
PRIMER_RIGHT_13=1818,20
PRIMER_LEFT_13_TM=58.989
PRIMER_RIGHT_13_TM=59.460
PRIMER_LEFT_13_GC_PERCENT=45.833
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=901
PRIMER_PAIR_14_PENALTY=5.600876
PRIMER_LEFT_14_PENALTY=5.010561
PRIMER_RIGHT_14_PENALTY=0.590315
PRIMER_LEFT_14_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_14_SEQUENCE=TCCAAGAGAGATCCCACCAA
PRIMER_LEFT_14=918,24
PRIMER_RIGHT_14=1817,20
PRIMER_LEFT_14_TM=58.989
PRIMER_RIGHT_14_TM=60.590
PRIMER_LEFT_14_GC_PERCENT=45.833
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=900
PRIMER_PAIR_15_PENALTY=5.691089
PRIMER_LEFT_15_PENALTY=4.851580
PRIMER_RIGHT_15_PENALTY=0.839509
PRIMER_LEFT_15_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_15_SEQUENCE=AAGACCATGAGTGGTGGTGG
PRIMER_LEFT_15=919,23
PRIMER_RIGHT_15=1587,20
PRIMER_LEFT_15_TM=58.148
PRIMER_RIGHT_15_TM=60.840
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=669
PRIMER_PAIR_16_PENALTY=5.726079
PRIMER_LEFT_16_PENALTY=4.851580
PRIMER_RIGHT_16_PENALTY=0.874500
PRIMER_LEFT_16_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_16_SEQUENCE=GGAGGTATTGCTGGGAAGAA
PRIMER_LEFT_16=919,23
PRIMER_RIGHT_16=1879,20
PRIMER_LEFT_16_TM=58.148
PRIMER_RIGHT_16_TM=59.126
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=961
PRIMER_PAIR_17_PENALTY=5.756714
PRIMER_LEFT_17_PENALTY=4.917205
PRIMER_RIGHT_17_PENALTY=0.839509
PRIMER_LEFT_17_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_17_SEQUENCE=AAGACCATGAGTGGTGGTGG
PRIMER_LEFT_17=920,22
PRIMER_RIGHT_17=1587,20
PRIMER_LEFT_17_TM=57.083
PRIMER_RIGHT_17_TM=60.840
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=668
PRIMER_PAIR_18_PENALTY=5.791705
PRIMER_LEFT_18_PENALTY=4.917205
PRIMER_RIGHT_18_PENALTY=0.874500
PRIMER_LEFT_18_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_18_SEQUENCE=GGAGGTATTGCTGGGAAGAA
PRIMER_LEFT_18=920,22
PRIMER_RIGHT_18=1879,20
PRIMER_LEFT_18_TM=57.083
PRIMER_RIGHT_18_TM=59.126
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=960
PRIMER_PAIR_19_PENALTY=5.805137
PRIMER_LEFT_19_PENALTY=4.851580
PRIMER_RIGHT_19_PENALTY=0.953558
PRIMER_LEFT_19_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_19_SEQUENCE=GATGGCTATGGCTGGTTTTT
PRIMER_LEFT_19=919,23
PRIMER_RIGHT_19=1769,20
PRIMER_LEFT_19_TM=58.148
PRIMER_RIGHT_19_TM=59.046
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=851
PRIMER_PAIR_20_PENALTY=5.850070
PRIMER_LEFT_20_PENALTY=5.010561
PRIMER_RIGHT_20_PENALTY=0.839509
PRIMER_LEFT_20_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_20_SEQUENCE=AAGACCATGAGTGGTGGTGG
PRIMER_LEFT_20=918,24
PRIMER_RIGHT_20=1587,20
PRIMER_LEFT_20_TM=58.989
PRIMER_RIGHT_20_TM=60.840
PRIMER_LEFT_20_GC_PERCENT=45.833
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=670
PRIMER_PAIR_21_PENALTY=5.870763
PRIMER_LEFT_21_PENALTY=4.917205
PRIMER_RIGHT_21_PENALTY=0.953558
PRIMER_LEFT_21_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_21_SEQUENCE=GATGGCTATGGCTGGTTTTT
PRIMER_LEFT_21=920,22
PRIMER_RIGHT_21=1769,20
PRIMER_LEFT_21_TM=57.083
PRIMER_RIGHT_21_TM=59.046
PRIMER_LEFT_21_GC_PERCENT=45.455
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=850
PRIMER_PAIR_22_PENALTY=5.872115
PRIMER_LEFT_22_PENALTY=4.851580
PRIMER_RIGHT_22_PENALTY=1.020535
PRIMER_LEFT_22_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_22_SEQUENCE=AGATGGCTATGGCTGGTTTTT
PRIMER_LEFT_22=919,23
PRIMER_RIGHT_22=1770,21
PRIMER_LEFT_22_TM=58.148
PRIMER_RIGHT_22_TM=59.979
PRIMER_LEFT_22_GC_PERCENT=47.826
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=852
PRIMER_PAIR_23_PENALTY=5.874498
PRIMER_LEFT_23_PENALTY=4.851580
PRIMER_RIGHT_23_PENALTY=1.022919
PRIMER_LEFT_23_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_23_SEQUENCE=AGATCATTGCTATGCGGTGG
PRIMER_LEFT_23=919,23
PRIMER_RIGHT_23=1381,20
PRIMER_LEFT_23_TM=58.148
PRIMER_RIGHT_23_TM=61.023
PRIMER_LEFT_23_GC_PERCENT=47.826
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=463
PRIMER_PAIR_24_PENALTY=5.885060
PRIMER_LEFT_24_PENALTY=5.010561
PRIMER_RIGHT_24_PENALTY=0.874500
PRIMER_LEFT_24_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_24_SEQUENCE=GGAGGTATTGCTGGGAAGAA
PRIMER_LEFT_24=918,24
PRIMER_RIGHT_24=1879,20
PRIMER_LEFT_24_TM=58.989
PRIMER_RIGHT_24_TM=59.126
PRIMER_LEFT_24_GC_PERCENT=45.833
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=962
PRIMER_PAIR_25_PENALTY=5.923307
PRIMER_LEFT_25_PENALTY=4.851580
PRIMER_RIGHT_25_PENALTY=1.071728
PRIMER_LEFT_25_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_25_SEQUENCE=GGTGGATCAACGGGCTTATT
PRIMER_LEFT_25=919,23
PRIMER_RIGHT_25=1366,20
PRIMER_LEFT_25_TM=58.148
PRIMER_RIGHT_25_TM=61.072
PRIMER_LEFT_25_GC_PERCENT=47.826
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=448
PRIMER_PAIR_26_PENALTY=5.937740
PRIMER_LEFT_26_PENALTY=4.917205
PRIMER_RIGHT_26_PENALTY=1.020535
PRIMER_LEFT_26_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_26_SEQUENCE=AGATGGCTATGGCTGGTTTTT
PRIMER_LEFT_26=920,22
PRIMER_RIGHT_26=1770,21
PRIMER_LEFT_26_TM=57.083
PRIMER_RIGHT_26_TM=59.979
PRIMER_LEFT_26_GC_PERCENT=45.455
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=851
PRIMER_PAIR_27_PENALTY=5.940124
PRIMER_LEFT_27_PENALTY=4.917205
PRIMER_RIGHT_27_PENALTY=1.022919
PRIMER_LEFT_27_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_27_SEQUENCE=AGATCATTGCTATGCGGTGG
PRIMER_LEFT_27=920,22
PRIMER_RIGHT_27=1381,20
PRIMER_LEFT_27_TM=57.083
PRIMER_RIGHT_27_TM=61.023
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=462
PRIMER_PAIR_28_PENALTY=5.959715
PRIMER_LEFT_28_PENALTY=4.851580
PRIMER_RIGHT_28_PENALTY=1.108135
PRIMER_LEFT_28_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_28_SEQUENCE=CATATTTGCCAGTGTTTGGG
PRIMER_LEFT_28=919,23
PRIMER_RIGHT_28=1715,20
PRIMER_LEFT_28_TM=58.148
PRIMER_RIGHT_28_TM=58.892
PRIMER_LEFT_28_GC_PERCENT=47.826
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=797
PRIMER_PAIR_29_PENALTY=5.964119
PRIMER_LEFT_29_PENALTY=5.010561
PRIMER_RIGHT_29_PENALTY=0.953558
PRIMER_LEFT_29_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_29_SEQUENCE=GATGGCTATGGCTGGTTTTT
PRIMER_LEFT_29=918,24
PRIMER_RIGHT_29=1769,20
PRIMER_LEFT_29_TM=58.989
PRIMER_RIGHT_29_TM=59.046
PRIMER_LEFT_29_GC_PERCENT=45.833
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=7.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=852
PRIMER_PAIR_30_PENALTY=5.988933
PRIMER_LEFT_30_PENALTY=4.917205
PRIMER_RIGHT_30_PENALTY=1.071728
PRIMER_LEFT_30_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_30_SEQUENCE=GGTGGATCAACGGGCTTATT
PRIMER_LEFT_30=920,22
PRIMER_RIGHT_30=1366,20
PRIMER_LEFT_30_TM=57.083
PRIMER_RIGHT_30_TM=61.072
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=6.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=447
PRIMER_PAIR_31_PENALTY=6.025340
PRIMER_LEFT_31_PENALTY=4.917205
PRIMER_RIGHT_31_PENALTY=1.108135
PRIMER_LEFT_31_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_31_SEQUENCE=CATATTTGCCAGTGTTTGGG
PRIMER_LEFT_31=920,22
PRIMER_RIGHT_31=1715,20
PRIMER_LEFT_31_TM=57.083
PRIMER_RIGHT_31_TM=58.892
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=10.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=796
PRIMER_PAIR_32_PENALTY=6.031096
PRIMER_LEFT_32_PENALTY=5.010561
PRIMER_RIGHT_32_PENALTY=1.020535
PRIMER_LEFT_32_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_32_SEQUENCE=AGATGGCTATGGCTGGTTTTT
PRIMER_LEFT_32=918,24
PRIMER_RIGHT_32=1770,21
PRIMER_LEFT_32_TM=58.989
PRIMER_RIGHT_32_TM=59.979
PRIMER_LEFT_32_GC_PERCENT=45.833
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=853
PRIMER_PAIR_33_PENALTY=6.033480
PRIMER_LEFT_33_PENALTY=5.010561
PRIMER_RIGHT_33_PENALTY=1.022919
PRIMER_LEFT_33_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_33_SEQUENCE=AGATCATTGCTATGCGGTGG
PRIMER_LEFT_33=918,24
PRIMER_RIGHT_33=1381,20
PRIMER_LEFT_33_TM=58.989
PRIMER_RIGHT_33_TM=61.023
PRIMER_LEFT_33_GC_PERCENT=45.833
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=464
PRIMER_PAIR_34_PENALTY=6.061410
PRIMER_LEFT_34_PENALTY=5.965639
PRIMER_RIGHT_34_PENALTY=0.095771
PRIMER_LEFT_34_SEQUENCE=TACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_34_SEQUENCE=CAGATGGCTATGGCTGGTTT
PRIMER_LEFT_34=917,25
PRIMER_RIGHT_34=1771,20
PRIMER_LEFT_34_TM=59.034
PRIMER_RIGHT_34_TM=60.096
PRIMER_LEFT_34_GC_PERCENT=44.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=855
PRIMER_PAIR_35_PENALTY=6.082288
PRIMER_LEFT_35_PENALTY=5.010561
PRIMER_RIGHT_35_PENALTY=1.071728
PRIMER_LEFT_35_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_35_SEQUENCE=GGTGGATCAACGGGCTTATT
PRIMER_LEFT_35=918,24
PRIMER_RIGHT_35=1366,20
PRIMER_LEFT_35_TM=58.989
PRIMER_RIGHT_35_TM=61.072
PRIMER_LEFT_35_GC_PERCENT=45.833
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=6.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=449
PRIMER_PAIR_36_PENALTY=6.113279
PRIMER_LEFT_36_PENALTY=4.851580
PRIMER_RIGHT_36_PENALTY=1.261699
PRIMER_LEFT_36_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_36_SEQUENCE=ACATATTTGCCAGTGTTTGGG
PRIMER_LEFT_36=919,23
PRIMER_RIGHT_36=1716,21
PRIMER_LEFT_36_TM=58.148
PRIMER_RIGHT_36_TM=59.738
PRIMER_LEFT_36_GC_PERCENT=47.826
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=10.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=798
PRIMER_PAIR_37_PENALTY=6.118696
PRIMER_LEFT_37_PENALTY=5.010561
PRIMER_RIGHT_37_PENALTY=1.108135
PRIMER_LEFT_37_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_37_SEQUENCE=CATATTTGCCAGTGTTTGGG
PRIMER_LEFT_37=918,24
PRIMER_RIGHT_37=1715,20
PRIMER_LEFT_37_TM=58.989
PRIMER_RIGHT_37_TM=58.892
PRIMER_LEFT_37_GC_PERCENT=45.833
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=10.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=798
PRIMER_PAIR_38_PENALTY=6.178905
PRIMER_LEFT_38_PENALTY=4.917205
PRIMER_RIGHT_38_PENALTY=1.261699
PRIMER_LEFT_38_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_38_SEQUENCE=ACATATTTGCCAGTGTTTGGG
PRIMER_LEFT_38=920,22
PRIMER_RIGHT_38=1716,21
PRIMER_LEFT_38_TM=57.083
PRIMER_RIGHT_38_TM=59.738
PRIMER_LEFT_38_GC_PERCENT=45.455
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=10.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=797
PRIMER_PAIR_39_PENALTY=6.179193
PRIMER_LEFT_39_PENALTY=4.851580
PRIMER_RIGHT_39_PENALTY=1.327613
PRIMER_LEFT_39_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_39_SEQUENCE=AGATGGCTATGGCTGGTTTT
PRIMER_LEFT_39=919,23
PRIMER_RIGHT_39=1770,20
PRIMER_LEFT_39_TM=58.148
PRIMER_RIGHT_39_TM=58.672
PRIMER_LEFT_39_GC_PERCENT=47.826
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=852
PRIMER_PAIR_40_PENALTY=6.191350
PRIMER_LEFT_40_PENALTY=6.095579
PRIMER_RIGHT_40_PENALTY=0.095771
PRIMER_LEFT_40_SEQUENCE=CTACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_40_SEQUENCE=CAGATGGCTATGGCTGGTTT
PRIMER_LEFT_40=916,26
PRIMER_RIGHT_40=1771,20
PRIMER_LEFT_40_TM=59.904
PRIMER_RIGHT_40_TM=60.096
PRIMER_LEFT_40_GC_PERCENT=46.154
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=856
PRIMER_PAIR_41_PENALTY=6.192995
PRIMER_LEFT_41_PENALTY=5.965639
PRIMER_RIGHT_41_PENALTY=0.227356
PRIMER_LEFT_41_SEQUENCE=TACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_41_SEQUENCE=TATTTGCCAGTGTTTGGGGT
PRIMER_LEFT_41=917,25
PRIMER_RIGHT_41=1713,20
PRIMER_LEFT_41_TM=59.034
PRIMER_RIGHT_41_TM=60.227
PRIMER_LEFT_41_GC_PERCENT=44.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=10.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=797
PRIMER_PAIR_42_PENALTY=6.244819
PRIMER_LEFT_42_PENALTY=4.917205
PRIMER_RIGHT_42_PENALTY=1.327613
PRIMER_LEFT_42_SEQUENCE=TGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_42_SEQUENCE=AGATGGCTATGGCTGGTTTT
PRIMER_LEFT_42=920,22
PRIMER_RIGHT_42=1770,20
PRIMER_LEFT_42_TM=57.083
PRIMER_RIGHT_42_TM=58.672
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=851
PRIMER_PAIR_43_PENALTY=6.272260
PRIMER_LEFT_43_PENALTY=5.010561
PRIMER_RIGHT_43_PENALTY=1.261699
PRIMER_LEFT_43_SEQUENCE=ACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_43_SEQUENCE=ACATATTTGCCAGTGTTTGGG
PRIMER_LEFT_43=918,24
PRIMER_RIGHT_43=1716,21
PRIMER_LEFT_43_TM=58.989
PRIMER_RIGHT_43_TM=59.738
PRIMER_LEFT_43_GC_PERCENT=45.833
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=10.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=799
PRIMER_PAIR_44_PENALTY=6.280610
PRIMER_LEFT_44_PENALTY=5.965639
PRIMER_RIGHT_44_PENALTY=0.314970
PRIMER_LEFT_44_SEQUENCE=TACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_44_SEQUENCE=ATATTTGCCAGTGTTTGGGG
PRIMER_LEFT_44=917,25
PRIMER_RIGHT_44=1714,20
PRIMER_LEFT_44_TM=59.034
PRIMER_RIGHT_44_TM=59.685
PRIMER_LEFT_44_GC_PERCENT=44.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=11.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=798
PRIMER_PAIR_45_PENALTY=6.285553
PRIMER_LEFT_45_PENALTY=4.851580
PRIMER_RIGHT_45_PENALTY=1.433974
PRIMER_LEFT_45_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_45_SEQUENCE=GACCATGAGTGGTGGTGGAG
PRIMER_LEFT_45=919,23
PRIMER_RIGHT_45=1585,20
PRIMER_LEFT_45_TM=58.148
PRIMER_RIGHT_45_TM=61.434
PRIMER_LEFT_45_GC_PERCENT=47.826
PRIMER_RIGHT_45_GC_PERCENT=60.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=667
PRIMER_PAIR_46_PENALTY=6.285553
PRIMER_LEFT_46_PENALTY=4.851580
PRIMER_RIGHT_46_PENALTY=1.433974
PRIMER_LEFT_46_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_46_SEQUENCE=AGACCATGAGTGGTGGTGGA
PRIMER_LEFT_46=919,23
PRIMER_RIGHT_46=1586,20
PRIMER_LEFT_46_TM=58.148
PRIMER_RIGHT_46_TM=61.434
PRIMER_LEFT_46_GC_PERCENT=47.826
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=668
PRIMER_PAIR_47_PENALTY=6.322935
PRIMER_LEFT_47_PENALTY=6.095579
PRIMER_RIGHT_47_PENALTY=0.227356
PRIMER_LEFT_47_SEQUENCE=CTACTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_47_SEQUENCE=TATTTGCCAGTGTTTGGGGT
PRIMER_LEFT_47=916,26
PRIMER_RIGHT_47=1713,20
PRIMER_LEFT_47_TM=59.904
PRIMER_RIGHT_47_TM=60.227
PRIMER_LEFT_47_GC_PERCENT=46.154
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=10.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=798
PRIMER_PAIR_48_PENALTY=6.324349
PRIMER_LEFT_48_PENALTY=4.851580
PRIMER_RIGHT_48_PENALTY=1.472769
PRIMER_LEFT_48_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_48_SEQUENCE=CGGTTGCATTAACGTACTTCC
PRIMER_LEFT_48=919,23
PRIMER_RIGHT_48=1620,21
PRIMER_LEFT_48_TM=58.148
PRIMER_RIGHT_48_TM=59.527
PRIMER_LEFT_48_GC_PERCENT=47.826
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=702
PRIMER_PAIR_49_PENALTY=6.328605
PRIMER_LEFT_49_PENALTY=4.851580
PRIMER_RIGHT_49_PENALTY=1.477025
PRIMER_LEFT_49_SEQUENCE=CTGTCTCACTATCGTTCTCAAGG
PRIMER_RIGHT_49_SEQUENCE=ATATTTGCCAGTGTTTGGGGT
PRIMER_LEFT_49=919,23
PRIMER_RIGHT_49=1714,21
PRIMER_LEFT_49_TM=58.148
PRIMER_RIGHT_49_TM=60.477
PRIMER_LEFT_49_GC_PERCENT=47.826
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=10.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=796
=
SEQUENCE_ID=MAGIC.105_4_2590253_2590563_tsu-0_zu-0_6019_189637_TRUE
SEQUENCE_TARGET=950,410
SEQUENCE_TEMPLATE=CGAAACAAATCGAAGAAATACAATAAGAANTCAGANATNTACCTTGATCACGGTNGAGANATTTGAGAGATTGATTTNAGAGTTNGAGNAANNTGGAATCTGAATTGTGAAGAAGAAGAGCTCTTTGGGTATTTGATTTATAGAAGCAANAAAGACGAGNACGNCTCGGTCGGTCACGAGAAAGCTAANGCGTGGAAGCAATAGCCAATTATANATTGACACGTATGTGTAGGGACGTGTACGCGTTGTTCACACGCANAGTTGNTTTTGNCTAATTGCCTTATTTTTAGNTTNAGGAAAAGTANTTTGTGCATTGAGTTGTNAAANATTNGATGTAAANATAGTTTGCTGCGAGACATATACTGAANGTTGAATTACNATTNNNNNAGNTCATTAGATNATCTNGATANGTATGCCAAGAAGTTCTCTGNGTNGAAANTTGAATTAAGCANAACTGAGAGACATNTACTGAAAGAGATGGGTTTTGTTTGTCATGTTGNANANTAGAAACACCTCCNGAATTGAGGCNNNAAGCTTGGAATTTNGCTAATGATAGTCTNCGTANANCCNTTTGTGTAAGGTTCACAAGTGAGGTTGTGGNTTGTGGGGTCGTGTATGCTGCTGCNCGTAGGTTTCAAGTACCTCTCCCNGAGAATNCACCCTGNTGNAANGCATTTGATGCAGATAAATCTANNNNNNNNNNNNNNNGNANNNTTNTNGNTCATCTATACACTCTTCCAAAGGCTTANTATATCTCNTNTGTTTGNAAGGATTCAGGTGGACANTCAGATAAATCAAGACATCACTCTTTTNCAAANATCCTTTTCANNNNNNNNNNNNNNNNNNNTCCTCNGCAAATNATTTTGGGATTACTAAGANAANTGATCATAAATACAATCTTCTATCTCNTCACACAACANATTTTGANTCGGCACAAANTNAAAAAANAAAAANACATNNACACACTTTTTGGTCTTGGTGGTNTTTAAGTTTCAAGCAGNGTCTACTCCTCCTTGTTGNTGTGCAAGATATCTCTCTCGGCGTTGCTTCTGGTCATTAGTACAGAAGATAAACACAGTNANCATCANTATCACANTTTCTTTGTTGTCTTNTAAAACAAAACANAAAAANAAGACNNGAGTAAGGCATGAAAANAATGGCATNCGTCTCTCACCCATTCATCGATCACAAGCCCTTCTCCAATTGGNTTTGGACCAACAATACCNTCTGATGCTTTNTTCCGTGCTTCATCTGCAGCATCCGCTTCAGCCAATTGACTCTTCAATTTCTCCATCATCTGTAAAGAGAGANGTAACAAAGNTTAAAGAATCAGTGATATCTANNATTNCTTAAAGCNNNAAATGATCCCAATCAAGTTCACAAATGATGGCGANAAGAANCAGAAAAAGAAGAAGAAGCNGACNGACTCACGGNGTTAGGGTTTTCGGGATCCAAGAATACCACACGCATTACTTGCTCCTCAGAGACATNATCTTTTTGCTCCTCAAACTGCAAAACACAAGAGCATCCACACTTCTTAGAATCCAANAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAGTTACTANAAGTTTCACATACCAGCTCAGGCATATAGTCTGTTGGNACTTTCATCTTCANGCTCATGATTTTAAACTTAACCCCGCTCTTTTGTTCCATNGGCTTTTCATTATTCTCAGGAGGCTCAACAAACTTGAAAACTAAAGAATTCAACAAGACATAGAAACNAACAGTCACCACANAATCNTNGAGGCCAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAGAAGCAAATACCAGTTGCNATAATTTCTTCCCCNGGAGCAAGAATAGCACCAGCNGGNCGCATGAAGCANCTCTTTGGTACTGTTGTTTGAAACTGNNNAAACCAAGATAANCAGCAGANATAAACACCAATNACAACAACAACAACCATAATCAAAACGTTTNTGAATCCATTNCAAACCTTAAACGCTACATGTGACTTGCTCGTATTCTTAATCTTAATNGCACTCCTGACTTGCTTCCCAGGCTCATCTACANACAAAGTTCATAAACTAAATNACTAATCTTNGATCATCNATAAAGAGTGAATCTTGAAATTGACAAGACTCCACATTTTATTCCTAAATTTGCTCAATGGCNNNNNNNNNTTTATNGTAACGTATCAATAAGTCAAATTACAAGTCAACATCATCATNNNCTTCAATATATATCCAATTTCATGATTCNCAAAGATAAAGCTAAACCAG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.043189
PRIMER_LEFT_0_PENALTY=2.931824
PRIMER_RIGHT_0_PENALTY=0.111365
PRIMER_LEFT_0_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_0_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_0=601,20
PRIMER_RIGHT_0=1391,20
PRIMER_LEFT_0_TM=62.932
PRIMER_RIGHT_0_TM=60.111
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=791
PRIMER_PAIR_1_PENALTY=3.346980
PRIMER_LEFT_1_PENALTY=2.931824
PRIMER_RIGHT_1_PENALTY=0.415156
PRIMER_LEFT_1_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_1_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_1=601,20
PRIMER_RIGHT_1=1513,20
PRIMER_LEFT_1_TM=62.932
PRIMER_RIGHT_1_TM=59.585
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=913
PRIMER_PAIR_2_PENALTY=3.346980
PRIMER_LEFT_2_PENALTY=2.931824
PRIMER_RIGHT_2_PENALTY=0.415156
PRIMER_LEFT_2_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_2_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_2=601,20
PRIMER_RIGHT_2=1514,20
PRIMER_LEFT_2_TM=62.932
PRIMER_RIGHT_2_TM=59.585
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=914
PRIMER_PAIR_3_PENALTY=3.507807
PRIMER_LEFT_3_PENALTY=3.396441
PRIMER_RIGHT_3_PENALTY=0.111365
PRIMER_LEFT_3_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_3_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_3=626,23
PRIMER_RIGHT_3=1391,20
PRIMER_LEFT_3_TM=60.396
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=766
PRIMER_PAIR_4_PENALTY=3.534415
PRIMER_LEFT_4_PENALTY=3.423050
PRIMER_RIGHT_4_PENALTY=0.111365
PRIMER_LEFT_4_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_4_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_4=603,20
PRIMER_RIGHT_4=1391,20
PRIMER_LEFT_4_TM=63.423
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=789
PRIMER_PAIR_5_PENALTY=3.751869
PRIMER_LEFT_5_PENALTY=3.640503
PRIMER_RIGHT_5_PENALTY=0.111365
PRIMER_LEFT_5_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_5_SEQUENCE=CGCCATCATTTGTGAACTTG
PRIMER_LEFT_5=479,20
PRIMER_RIGHT_5=1391,20
PRIMER_LEFT_5_TM=56.359
PRIMER_RIGHT_5_TM=60.111
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=913
PRIMER_PAIR_6_PENALTY=3.811598
PRIMER_LEFT_6_PENALTY=3.396441
PRIMER_RIGHT_6_PENALTY=0.415156
PRIMER_LEFT_6_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_6_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_6=626,23
PRIMER_RIGHT_6=1513,20
PRIMER_LEFT_6_TM=60.396
PRIMER_RIGHT_6_TM=59.585
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=888
PRIMER_PAIR_7_PENALTY=3.811598
PRIMER_LEFT_7_PENALTY=3.396441
PRIMER_RIGHT_7_PENALTY=0.415156
PRIMER_LEFT_7_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_7_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_7=626,23
PRIMER_RIGHT_7=1514,20
PRIMER_LEFT_7_TM=60.396
PRIMER_RIGHT_7_TM=59.585
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=889
PRIMER_PAIR_8_PENALTY=3.881760
PRIMER_LEFT_8_PENALTY=2.931824
PRIMER_RIGHT_8_PENALTY=0.949937
PRIMER_LEFT_8_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_8_SEQUENCE=GTTTTGCAGTTTGAGGAGCA
PRIMER_LEFT_8=601,20
PRIMER_RIGHT_8=1517,20
PRIMER_LEFT_8_TM=62.932
PRIMER_RIGHT_8_TM=59.050
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=917
PRIMER_PAIR_9_PENALTY=3.929988
PRIMER_LEFT_9_PENALTY=2.931824
PRIMER_RIGHT_9_PENALTY=0.998165
PRIMER_LEFT_9_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_9_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_9=601,20
PRIMER_RIGHT_9=1469,20
PRIMER_LEFT_9_TM=62.932
PRIMER_RIGHT_9_TM=59.002
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=2.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=869
PRIMER_PAIR_10_PENALTY=3.973793
PRIMER_LEFT_10_PENALTY=2.931824
PRIMER_RIGHT_10_PENALTY=1.041969
PRIMER_LEFT_10_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_10_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_10=601,20
PRIMER_RIGHT_10=1453,20
PRIMER_LEFT_10_TM=62.932
PRIMER_RIGHT_10_TM=61.042
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=5.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=853
PRIMER_PAIR_11_PENALTY=3.979923
PRIMER_LEFT_11_PENALTY=2.931824
PRIMER_RIGHT_11_PENALTY=1.048100
PRIMER_LEFT_11_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_11_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_11=601,20
PRIMER_RIGHT_11=1480,20
PRIMER_LEFT_11_TM=62.932
PRIMER_RIGHT_11_TM=58.952
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=880
PRIMER_PAIR_12_PENALTY=4.127860
PRIMER_LEFT_12_PENALTY=2.931824
PRIMER_RIGHT_12_PENALTY=1.196037
PRIMER_LEFT_12_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_12_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_12=601,20
PRIMER_RIGHT_12=1478,20
PRIMER_LEFT_12_TM=62.932
PRIMER_RIGHT_12_TM=58.804
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=878
PRIMER_PAIR_13_PENALTY=4.183085
PRIMER_LEFT_13_PENALTY=2.931824
PRIMER_RIGHT_13_PENALTY=1.251261
PRIMER_LEFT_13_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_13_SEQUENCE=TGTGGATGCTCTTGTGTTTTG
PRIMER_LEFT_13=601,20
PRIMER_RIGHT_13=1532,21
PRIMER_LEFT_13_TM=62.932
PRIMER_RIGHT_13_TM=59.749
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=932
PRIMER_PAIR_14_PENALTY=4.215867
PRIMER_LEFT_14_PENALTY=3.800711
PRIMER_RIGHT_14_PENALTY=0.415156
PRIMER_LEFT_14_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_14_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_14=574,23
PRIMER_RIGHT_14=1513,20
PRIMER_LEFT_14_TM=59.199
PRIMER_RIGHT_14_TM=59.585
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=940
PRIMER_PAIR_15_PENALTY=4.215867
PRIMER_LEFT_15_PENALTY=3.800711
PRIMER_RIGHT_15_PENALTY=0.415156
PRIMER_LEFT_15_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_15_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_15=574,23
PRIMER_RIGHT_15=1514,20
PRIMER_LEFT_15_TM=59.199
PRIMER_RIGHT_15_TM=59.585
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=941
PRIMER_PAIR_16_PENALTY=4.308600
PRIMER_LEFT_16_PENALTY=2.931824
PRIMER_RIGHT_16_PENALTY=1.376776
PRIMER_LEFT_16_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_16_SEQUENCE=GCAGTTTGAGGAGCAAAAAGA
PRIMER_LEFT_16=601,20
PRIMER_RIGHT_16=1512,21
PRIMER_LEFT_16_TM=62.932
PRIMER_RIGHT_16_TM=59.623
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=912
PRIMER_PAIR_17_PENALTY=4.311754
PRIMER_LEFT_17_PENALTY=2.931824
PRIMER_RIGHT_17_PENALTY=1.379931
PRIMER_LEFT_17_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_17_SEQUENCE=AATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_17=601,20
PRIMER_RIGHT_17=1470,21
PRIMER_LEFT_17_TM=62.932
PRIMER_RIGHT_17_TM=60.380
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=2.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=870
PRIMER_PAIR_18_PENALTY=4.346378
PRIMER_LEFT_18_PENALTY=3.396441
PRIMER_RIGHT_18_PENALTY=0.949937
PRIMER_LEFT_18_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_18_SEQUENCE=GTTTTGCAGTTTGAGGAGCA
PRIMER_LEFT_18=626,23
PRIMER_RIGHT_18=1517,20
PRIMER_LEFT_18_TM=60.396
PRIMER_RIGHT_18_TM=59.050
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=892
PRIMER_PAIR_19_PENALTY=4.394606
PRIMER_LEFT_19_PENALTY=3.396441
PRIMER_RIGHT_19_PENALTY=0.998165
PRIMER_LEFT_19_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_19_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_19=626,23
PRIMER_RIGHT_19=1469,20
PRIMER_LEFT_19_TM=60.396
PRIMER_RIGHT_19_TM=59.002
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=844
PRIMER_PAIR_20_PENALTY=4.415611
PRIMER_LEFT_20_PENALTY=4.000455
PRIMER_RIGHT_20_PENALTY=0.415156
PRIMER_LEFT_20_SEQUENCE=GTGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_20_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_20=573,24
PRIMER_RIGHT_20=1513,20
PRIMER_LEFT_20_TM=60.000
PRIMER_RIGHT_20_TM=59.585
PRIMER_LEFT_20_GC_PERCENT=45.833
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=941
PRIMER_PAIR_21_PENALTY=4.415611
PRIMER_LEFT_21_PENALTY=4.000455
PRIMER_RIGHT_21_PENALTY=0.415156
PRIMER_LEFT_21_SEQUENCE=GTGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_21_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_21=573,24
PRIMER_RIGHT_21=1514,20
PRIMER_LEFT_21_TM=60.000
PRIMER_RIGHT_21_TM=59.585
PRIMER_LEFT_21_GC_PERCENT=45.833
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=942
PRIMER_PAIR_22_PENALTY=4.421215
PRIMER_LEFT_22_PENALTY=3.423050
PRIMER_RIGHT_22_PENALTY=0.998165
PRIMER_LEFT_22_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_22_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_22=603,20
PRIMER_RIGHT_22=1469,20
PRIMER_LEFT_22_TM=63.423
PRIMER_RIGHT_22_TM=59.002
PRIMER_LEFT_22_GC_PERCENT=60.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=867
PRIMER_PAIR_23_PENALTY=4.429486
PRIMER_LEFT_23_PENALTY=2.931824
PRIMER_RIGHT_23_PENALTY=1.497663
PRIMER_LEFT_23_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_23_SEQUENCE=AATGCGTGTGGTATTCTTGG
PRIMER_LEFT_23=601,20
PRIMER_RIGHT_23=1470,20
PRIMER_LEFT_23_TM=62.932
PRIMER_RIGHT_23_TM=58.502
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=870
PRIMER_PAIR_24_PENALTY=4.438410
PRIMER_LEFT_24_PENALTY=3.396441
PRIMER_RIGHT_24_PENALTY=1.041969
PRIMER_LEFT_24_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_24_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_24=626,23
PRIMER_RIGHT_24=1453,20
PRIMER_LEFT_24_TM=60.396
PRIMER_RIGHT_24_TM=61.042
PRIMER_LEFT_24_GC_PERCENT=52.174
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=5.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=828
PRIMER_PAIR_25_PENALTY=4.443762
PRIMER_LEFT_25_PENALTY=2.931824
PRIMER_RIGHT_25_PENALTY=1.511938
PRIMER_LEFT_25_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_25_SEQUENCE=TTGGATCCCGAAAACCCTAA
PRIMER_LEFT_25=601,20
PRIMER_RIGHT_25=1454,20
PRIMER_LEFT_25_TM=62.932
PRIMER_RIGHT_25_TM=61.512
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=7.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=854
PRIMER_PAIR_26_PENALTY=4.444541
PRIMER_LEFT_26_PENALTY=3.396441
PRIMER_RIGHT_26_PENALTY=1.048100
PRIMER_LEFT_26_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_26_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_26=626,23
PRIMER_RIGHT_26=1480,20
PRIMER_LEFT_26_TM=60.396
PRIMER_RIGHT_26_TM=58.952
PRIMER_LEFT_26_GC_PERCENT=52.174
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=855
PRIMER_PAIR_27_PENALTY=4.465019
PRIMER_LEFT_27_PENALTY=3.423050
PRIMER_RIGHT_27_PENALTY=1.041969
PRIMER_LEFT_27_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_27_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_27=603,20
PRIMER_RIGHT_27=1453,20
PRIMER_LEFT_27_TM=63.423
PRIMER_RIGHT_27_TM=61.042
PRIMER_LEFT_27_GC_PERCENT=60.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=5.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=851
PRIMER_PAIR_28_PENALTY=4.471150
PRIMER_LEFT_28_PENALTY=3.423050
PRIMER_RIGHT_28_PENALTY=1.048100
PRIMER_LEFT_28_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_28_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_28=603,20
PRIMER_RIGHT_28=1480,20
PRIMER_LEFT_28_TM=63.423
PRIMER_RIGHT_28_TM=58.952
PRIMER_LEFT_28_GC_PERCENT=60.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=878
PRIMER_PAIR_29_PENALTY=4.471867
PRIMER_LEFT_29_PENALTY=2.931824
PRIMER_RIGHT_29_PENALTY=1.540043
PRIMER_LEFT_29_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_29_SEQUENCE=TGCAGTTTGAGGAGCAAAAAG
PRIMER_LEFT_29=601,20
PRIMER_RIGHT_29=1513,21
PRIMER_LEFT_29_TM=62.932
PRIMER_RIGHT_29_TM=60.540
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=913
PRIMER_PAIR_30_PENALTY=4.562495
PRIMER_LEFT_30_PENALTY=2.931824
PRIMER_RIGHT_30_PENALTY=1.630671
PRIMER_LEFT_30_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_30_SEQUENCE=GGAGCAAGTAATGCGTGTGG
PRIMER_LEFT_30=601,20
PRIMER_RIGHT_30=1479,20
PRIMER_LEFT_30_TM=62.932
PRIMER_RIGHT_30_TM=61.631
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=879
PRIMER_PAIR_31_PENALTY=4.592478
PRIMER_LEFT_31_PENALTY=3.396441
PRIMER_RIGHT_31_PENALTY=1.196037
PRIMER_LEFT_31_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_31_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_31=626,23
PRIMER_RIGHT_31=1478,20
PRIMER_LEFT_31_TM=60.396
PRIMER_RIGHT_31_TM=58.804
PRIMER_LEFT_31_GC_PERCENT=52.174
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=853
PRIMER_PAIR_32_PENALTY=4.619087
PRIMER_LEFT_32_PENALTY=3.423050
PRIMER_RIGHT_32_PENALTY=1.196037
PRIMER_LEFT_32_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_32_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_32=603,20
PRIMER_RIGHT_32=1478,20
PRIMER_LEFT_32_TM=63.423
PRIMER_RIGHT_32_TM=58.804
PRIMER_LEFT_32_GC_PERCENT=60.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=876
PRIMER_PAIR_33_PENALTY=4.638668
PRIMER_LEFT_33_PENALTY=3.640503
PRIMER_RIGHT_33_PENALTY=0.998165
PRIMER_LEFT_33_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_33_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_33=479,20
PRIMER_RIGHT_33=1469,20
PRIMER_LEFT_33_TM=56.359
PRIMER_RIGHT_33_TM=59.002
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=991
PRIMER_PAIR_34_PENALTY=4.647703
PRIMER_LEFT_34_PENALTY=3.396441
PRIMER_RIGHT_34_PENALTY=1.251261
PRIMER_LEFT_34_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_34_SEQUENCE=TGTGGATGCTCTTGTGTTTTG
PRIMER_LEFT_34=626,23
PRIMER_RIGHT_34=1532,21
PRIMER_LEFT_34_TM=60.396
PRIMER_RIGHT_34_TM=59.749
PRIMER_LEFT_34_GC_PERCENT=52.174
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=907
PRIMER_PAIR_35_PENALTY=4.674311
PRIMER_LEFT_35_PENALTY=3.423050
PRIMER_RIGHT_35_PENALTY=1.251261
PRIMER_LEFT_35_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_35_SEQUENCE=TGTGGATGCTCTTGTGTTTTG
PRIMER_LEFT_35=603,20
PRIMER_RIGHT_35=1532,21
PRIMER_LEFT_35_TM=63.423
PRIMER_RIGHT_35_TM=59.749
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=930
PRIMER_PAIR_36_PENALTY=4.682473
PRIMER_LEFT_36_PENALTY=3.640503
PRIMER_RIGHT_36_PENALTY=1.041969
PRIMER_LEFT_36_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_36_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_36=479,20
PRIMER_RIGHT_36=1453,20
PRIMER_LEFT_36_TM=56.359
PRIMER_RIGHT_36_TM=61.042
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=5.7000
PRIMER_PAIR_36_COMPL_ANY=7.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=975
PRIMER_PAIR_37_PENALTY=4.727462
PRIMER_LEFT_37_PENALTY=4.312305
PRIMER_RIGHT_37_PENALTY=0.415156
PRIMER_LEFT_37_SEQUENCE=TGTGTAAGGTTCACAAGTGAGGT
PRIMER_RIGHT_37_SEQUENCE=TGCAGTTTGAGGAGCAAAAA
PRIMER_LEFT_37=572,23
PRIMER_RIGHT_37=1513,20
PRIMER_LEFT_37_TM=58.688
PRIMER_RIGHT_37_TM=59.585
PRIMER_LEFT_37_GC_PERCENT=43.478
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=942
PRIMER_PAIR_38_PENALTY=4.727462
PRIMER_LEFT_38_PENALTY=4.312305
PRIMER_RIGHT_38_PENALTY=0.415156
PRIMER_LEFT_38_SEQUENCE=TGTGTAAGGTTCACAAGTGAGGT
PRIMER_RIGHT_38_SEQUENCE=TTGCAGTTTGAGGAGCAAAA
PRIMER_LEFT_38=572,23
PRIMER_RIGHT_38=1514,20
PRIMER_LEFT_38_TM=58.688
PRIMER_RIGHT_38_TM=59.585
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.6000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=943
PRIMER_PAIR_39_PENALTY=4.750648
PRIMER_LEFT_39_PENALTY=3.800711
PRIMER_RIGHT_39_PENALTY=0.949937
PRIMER_LEFT_39_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_39_SEQUENCE=GTTTTGCAGTTTGAGGAGCA
PRIMER_LEFT_39=574,23
PRIMER_RIGHT_39=1517,20
PRIMER_LEFT_39_TM=59.199
PRIMER_RIGHT_39_TM=59.050
PRIMER_LEFT_39_GC_PERCENT=43.478
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=944
PRIMER_PAIR_40_PENALTY=4.773217
PRIMER_LEFT_40_PENALTY=3.396441
PRIMER_RIGHT_40_PENALTY=1.376776
PRIMER_LEFT_40_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_40_SEQUENCE=GCAGTTTGAGGAGCAAAAAGA
PRIMER_LEFT_40=626,23
PRIMER_RIGHT_40=1512,21
PRIMER_LEFT_40_TM=60.396
PRIMER_RIGHT_40_TM=59.623
PRIMER_LEFT_40_GC_PERCENT=52.174
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=887
PRIMER_PAIR_41_PENALTY=4.774083
PRIMER_LEFT_41_PENALTY=2.931824
PRIMER_RIGHT_41_PENALTY=1.842260
PRIMER_LEFT_41_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_41_SEQUENCE=GAGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_41=601,20
PRIMER_RIGHT_41=1481,21
PRIMER_LEFT_41_TM=62.932
PRIMER_RIGHT_41_TM=60.842
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=881
PRIMER_PAIR_42_PENALTY=4.774083
PRIMER_LEFT_42_PENALTY=2.931824
PRIMER_RIGHT_42_PENALTY=1.842260
PRIMER_LEFT_42_SEQUENCE=TTGTGGGGTCGTGTATGCTG
PRIMER_RIGHT_42_SEQUENCE=TGAGGAGCAAGTAATGCGTGT
PRIMER_LEFT_42=601,20
PRIMER_RIGHT_42=1482,21
PRIMER_LEFT_42_TM=62.932
PRIMER_RIGHT_42_TM=60.842
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=8.1000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=882
PRIMER_PAIR_43_PENALTY=4.776372
PRIMER_LEFT_43_PENALTY=3.396441
PRIMER_RIGHT_43_PENALTY=1.379931
PRIMER_LEFT_43_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_43_SEQUENCE=AATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_43=626,23
PRIMER_RIGHT_43=1470,21
PRIMER_LEFT_43_TM=60.396
PRIMER_RIGHT_43_TM=60.380
PRIMER_LEFT_43_GC_PERCENT=52.174
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=845
PRIMER_PAIR_44_PENALTY=4.798876
PRIMER_LEFT_44_PENALTY=3.800711
PRIMER_RIGHT_44_PENALTY=0.998165
PRIMER_LEFT_44_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_44_SEQUENCE=ATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_44=574,23
PRIMER_RIGHT_44=1469,20
PRIMER_LEFT_44_TM=59.199
PRIMER_RIGHT_44_TM=59.002
PRIMER_LEFT_44_GC_PERCENT=43.478
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=896
PRIMER_PAIR_45_PENALTY=4.802980
PRIMER_LEFT_45_PENALTY=3.423050
PRIMER_RIGHT_45_PENALTY=1.379931
PRIMER_LEFT_45_SEQUENCE=GTGGGGTCGTGTATGCTGCT
PRIMER_RIGHT_45_SEQUENCE=AATGCGTGTGGTATTCTTGGA
PRIMER_LEFT_45=603,20
PRIMER_RIGHT_45=1470,21
PRIMER_LEFT_45_TM=63.423
PRIMER_RIGHT_45_TM=60.380
PRIMER_LEFT_45_GC_PERCENT=60.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=868
PRIMER_PAIR_46_PENALTY=4.836540
PRIMER_LEFT_46_PENALTY=3.640503
PRIMER_RIGHT_46_PENALTY=1.196037
PRIMER_LEFT_46_SEQUENCE=GGGTTTTGTTTGTCATGTTG
PRIMER_RIGHT_46_SEQUENCE=GAGCAAGTAATGCGTGTGGT
PRIMER_LEFT_46=479,20
PRIMER_RIGHT_46=1478,20
PRIMER_LEFT_46_TM=56.359
PRIMER_RIGHT_46_TM=58.804
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=1000
PRIMER_PAIR_47_PENALTY=4.842680
PRIMER_LEFT_47_PENALTY=3.800711
PRIMER_RIGHT_47_PENALTY=1.041969
PRIMER_LEFT_47_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_47_SEQUENCE=TGGATCCCGAAAACCCTAAC
PRIMER_LEFT_47=574,23
PRIMER_RIGHT_47=1453,20
PRIMER_LEFT_47_TM=59.199
PRIMER_RIGHT_47_TM=61.042
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=5.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=880
PRIMER_PAIR_48_PENALTY=4.848811
PRIMER_LEFT_48_PENALTY=3.800711
PRIMER_RIGHT_48_PENALTY=1.048100
PRIMER_LEFT_48_SEQUENCE=TGTAAGGTTCACAAGTGAGGTTG
PRIMER_RIGHT_48_SEQUENCE=AGGAGCAAGTAATGCGTGTG
PRIMER_LEFT_48=574,23
PRIMER_RIGHT_48=1480,20
PRIMER_LEFT_48_TM=59.199
PRIMER_RIGHT_48_TM=58.952
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=907
PRIMER_PAIR_49_PENALTY=4.894104
PRIMER_LEFT_49_PENALTY=3.396441
PRIMER_RIGHT_49_PENALTY=1.497663
PRIMER_LEFT_49_SEQUENCE=CGTAGGTTTCAAGTACCTCTCCC
PRIMER_RIGHT_49_SEQUENCE=AATGCGTGTGGTATTCTTGG
PRIMER_LEFT_49=626,23
PRIMER_RIGHT_49=1470,20
PRIMER_LEFT_49_TM=60.396
PRIMER_RIGHT_49_TM=58.502
PRIMER_LEFT_49_GC_PERCENT=52.174
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=845
=
SEQUENCE_ID=MAGIC.105_2_3218569_3218700_wil-2_tsu-0_93793_282667_TRUE
SEQUENCE_TARGET=950,231
SEQUENCE_TEMPLATE=CATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGATACTTGNTTANCNTAATANTTATNTACNCATNNGTTTNGTAAANGATAANTATTTANACTTGTNTNCATATCANNNNAGTNTACTAANTATNTATACATCNAAAATACTNTAGAAATTATGTTTAAAGNANNATCAANNATTTTNTTTTTTGGTCTGCCAAATATTNCATTANAAACNANNGNNNNNNNANATTNAATCCNACCCANNNNNCAAATNTACTTCTCTTTTAATAGTNTANNNNTTNATTATACNTTTTTGGNTGCATTTTANAAGTTTTTTAAAGATANTTTTGTGCCAGTTNCTATANAAAANTTATATNNAAAANNNNNNATTNNTTNTTTTAATNNAAATATTAAATNATATTTTGGTATAAGAAAAANGTTTTCATATGCCTNCTTTAAGCCAAAAATCTNGTAAAATGGGACATAGGAAATANATATGTTATCTTATGGTTAACTTTCAAACNACTCTTGGTAANNNNNNNNNAAATAGTTTTNAAGTACAATTTTATTAAAANACTCTNGGTNNNNAGNNCATGATTGGTTGGGCTGTAAGAAATTTTTGTAGAAATAACAGATTGCCATNATCCTCTCATCGNACTGNNNNNNCTGGTTAANTTGTGAAAATTGANAGATTNACTTTGAGNTNANNNNNNAANNNNNGNCTTGANGNNNCTAGTACTNTTNTAAGCATTGNCTANCTCNTTTNNNTCNTGTNGTTCCATTGNTTNTTACCNGTNNNNNNNNNNNNNNNNNNNNNTANTANTACTCGNTTATCGAAAANTTTATTACTAGCTNGCACTCTCATTCACTCTTGTTNNTTTNTTGGTTACACTTNAGNGATTNATTAATAAATCTTGTTTTAATNNNNNNNNNNNNNNNNNNNNNNNNTGCTTTGAGATTGAATTGATGANTNGACTCAANAATATGTTATGCTTNNCTGCTTTNATNNTTTTNTTGGCATGNAACCATGTAATANNTTATNTTTCATNTANCCTTAACTTTNATTTGNNAAATGANNNNNNACATTTTTACCNAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTATATGNATANNTAAAACANGNAGTAGAAGTTAAACATTATGATTATTCAANAATTTAACTANTTAATANTCNTGAACTTGTTTATTTATANTTTTNAAAATTAGCTGTTACTAANNAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATAACATGNGTTTTGTTTCTNTGATAATTAATTTGNNTTNTNTNTTTGATGAACTAANNGGAANTTTGGATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCAGATGTCGAAANGCNTCACGTTC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_1023537_1023566_edi-0_can-0_397135_399_TRUE
SEQUENCE_TARGET=950,129
SEQUENCE_TEMPLATE=GGNGNTTCNAAACANGTTTTAGANACCCAACCTAGTCCAACNGGAACAATTTGAACCAACACTGAACCNGGTNGAAGAAACAACAANTGNGTTAATGCTGCACCATGNACCCCGANCATNCCGTGGCTNGACTTTATAAGTTTATANNCTTCTCTNAAACTCGTTGTTTTNGATGGTCTAAANGTGATTANCTCGAATCCAACATCTTCAAGCATCTCTNTGATCTCTTNCTCGTTTAATANNACTCGACCAATGTTACCATATCNACNGACCAATATGAGACGAGGCTTGTTAATTTTGATAATAGATAGNTTTGGGTTCAAGGCTTTGTCTAGGAGATTATGGAAGTCNACTAATGATTTGGAGTTGGGTATTTGGGTTGGGTCTATTNTCATAGGNCCATGTGAAATTAGGCCCACAGTGGCTGAAGTGAAGCAATGTGTAATAGAANCATTTTCTTTGTCTAGTAATATNGTTTTGTGTTTGCTAAACGTACCTAGNATATCNATGTATTTTGGCATCCACCATTCCTTNGNATTCACAACCACNAGGATGAAATNGCGGTCNGGGTATACCNAGTTNGCCGTGATAAAAAGNGGNATGAAACCATCGATAAAATCNTGATAAATNCTTCCGGTGTAACCTCCAGCACTGAACNCAATGNCCGGNGAATCATGTGTTATNNNNNNNNNNNNNNNNNNNNNNNATGGGCNTGANGNTAGTTTTAGCTCTCTGATCNTAGGCATGATCCANTTTTCTGNTTTTCTTGGNTATGGCCTGATTTTCTCGACAAGTGGTGCNNNTGTGGTNANTGTTNGGTCNATNAGAGTTANANTTCCGGTTTTNGNGTTGAGAATACATGAGCCGTTGATGGAGCAAAGATCNTAACTAGTGTGGGATCGGTCACATGTGATTTTTGCTGATGNAGCCATCACTTNNGGGTTTTGCTTTGTTCTTNTNGATTCCCATCTTTGTTGTAACGTACCTACAGAGGAAANTTNTTTNANNTNNTNNNNNNAAACNNNNNGTNAANNTNTNTAAGANANNANTNAGNAANTANTAAAANAGNTTTNNNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNTNNTNNATGNANNANNANTNCANATGNTTANAANNNAANNNNNNNNNNNAANTNAANNCNCNTNNAANANATTANNNNGNNTTANNNTNNTNANANANCNTNNCGNNNTAANTATNNANNANNCNATNANGTAANCTNTTANNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAANNNNNANNTNACANNNNNNNAANTNNNATTANANNNAAAANTCATTANNNNNNNNNNTTAGTTGCTTCTTTTGANCGGANTTTAGAATTANTGNNGTTTCGTAATGTTAGCATTTAGATTCAAATCCAGCCAAGTATGTGTGAGTTATTGCAAAAATGTTGACTNGATCGTTAAAACANTAGGTAATATAAAAGNAAAAGTGTTTGATCNTAGCCTACGACTCAANCAAGAAGTTNAAGNTTTATNTGGTTGAATCGAAGANGTAATTTGNTATNTACATACCTTGAGAGAAACTTNACGAANGAAACAATTGACGATCGNAGACGGAGGAGAAAGCAACATATATAACCATAACGATCACCAACGCCACCGAATAACTCTTCGACGTCNTTTTNNTAACCATGNNTTATATGCANATCNACCTTTAAGAATCCTTCAATNNAAGAGAAAAGAAAGAANAANNTTGTAAGTCNANTANNGGNTGAANAACAGAAGAAGATAGAATGNAAACGAGANCANTGATTCAGTATANNTGTANTTTTATCNAGAAGACTTTTCTCTTACAAAATATTTTTAATTGTTTGNAANNNTTTTTGCNANAAAAANAAAANNNTTTNCNTAATGCTGAGGNATGGGAATACTATAATAGAACAAATANGTNATAACATATCTCAAANGGAGTNNACGTTTACAGC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.459379
PRIMER_LEFT_0_PENALTY=0.327117
PRIMER_RIGHT_0_PENALTY=0.132262
PRIMER_LEFT_0_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_0_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_0=856,20
PRIMER_RIGHT_0=1714,20
PRIMER_LEFT_0_TM=59.673
PRIMER_RIGHT_0_TM=60.132
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=859
PRIMER_PAIR_1_PENALTY=1.113040
PRIMER_LEFT_1_PENALTY=0.327117
PRIMER_RIGHT_1_PENALTY=0.785923
PRIMER_LEFT_1_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_1_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_1=856,20
PRIMER_RIGHT_1=1700,20
PRIMER_LEFT_1_TM=59.673
PRIMER_RIGHT_1_TM=60.786
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=845
PRIMER_PAIR_2_PENALTY=1.128746
PRIMER_LEFT_2_PENALTY=0.327117
PRIMER_RIGHT_2_PENALTY=0.801629
PRIMER_LEFT_2_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_2_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_2=856,20
PRIMER_RIGHT_2=1716,20
PRIMER_LEFT_2_TM=59.673
PRIMER_RIGHT_2_TM=59.198
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=861
PRIMER_PAIR_3_PENALTY=1.606871
PRIMER_LEFT_3_PENALTY=0.327117
PRIMER_RIGHT_3_PENALTY=1.279754
PRIMER_LEFT_3_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_3_SEQUENCE=AAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_3=856,20
PRIMER_RIGHT_3=1715,20
PRIMER_LEFT_3_TM=59.673
PRIMER_RIGHT_3_TM=58.720
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=860
PRIMER_PAIR_4_PENALTY=1.723828
PRIMER_LEFT_4_PENALTY=1.713283
PRIMER_RIGHT_4_PENALTY=0.010545
PRIMER_LEFT_4_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_4_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_4=850,21
PRIMER_RIGHT_4=1674,20
PRIMER_LEFT_4_TM=59.287
PRIMER_RIGHT_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=825
PRIMER_PAIR_5_PENALTY=1.823992
PRIMER_LEFT_5_PENALTY=0.327117
PRIMER_RIGHT_5_PENALTY=1.496875
PRIMER_LEFT_5_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_5_SEQUENCE=GAAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_5=856,20
PRIMER_RIGHT_5=1716,21
PRIMER_LEFT_5_TM=59.673
PRIMER_RIGHT_5_TM=60.497
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=861
PRIMER_PAIR_6_PENALTY=1.845545
PRIMER_LEFT_6_PENALTY=1.713283
PRIMER_RIGHT_6_PENALTY=0.132262
PRIMER_LEFT_6_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_6_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_6=850,21
PRIMER_RIGHT_6=1714,20
PRIMER_LEFT_6_TM=59.287
PRIMER_RIGHT_6_TM=60.132
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=865
PRIMER_PAIR_7_PENALTY=1.974890
PRIMER_LEFT_7_PENALTY=0.327117
PRIMER_RIGHT_7_PENALTY=1.647773
PRIMER_LEFT_7_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_7_SEQUENCE=CGTTGGTGATCGTTATGGTT
PRIMER_LEFT_7=856,20
PRIMER_RIGHT_7=1699,20
PRIMER_LEFT_7_TM=59.673
PRIMER_RIGHT_7_TM=58.352
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=844
PRIMER_PAIR_8_PENALTY=1.995775
PRIMER_LEFT_8_PENALTY=0.327117
PRIMER_RIGHT_8_PENALTY=1.668658
PRIMER_LEFT_8_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_8_SEQUENCE=AGTTATTCGGTGGCGTTGGT
PRIMER_LEFT_8=856,20
PRIMER_RIGHT_8=1712,20
PRIMER_LEFT_8_TM=59.673
PRIMER_RIGHT_8_TM=61.669
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=857
PRIMER_PAIR_9_PENALTY=2.225735
PRIMER_LEFT_9_PENALTY=2.215190
PRIMER_RIGHT_9_PENALTY=0.010545
PRIMER_LEFT_9_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_9_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_9=849,21
PRIMER_RIGHT_9=1674,20
PRIMER_LEFT_9_TM=58.785
PRIMER_RIGHT_9_TM=59.989
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.7000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=826
PRIMER_PAIR_10_PENALTY=2.347452
PRIMER_LEFT_10_PENALTY=2.215190
PRIMER_RIGHT_10_PENALTY=0.132262
PRIMER_LEFT_10_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_10_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_10=849,21
PRIMER_RIGHT_10=1714,20
PRIMER_LEFT_10_TM=58.785
PRIMER_RIGHT_10_TM=60.132
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.7000
PRIMER_RIGHT_10_END_STABILITY=8.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=866
PRIMER_PAIR_11_PENALTY=2.373308
PRIMER_LEFT_11_PENALTY=2.362762
PRIMER_RIGHT_11_PENALTY=0.010545
PRIMER_LEFT_11_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_11_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_11=848,22
PRIMER_RIGHT_11=1674,20
PRIMER_LEFT_11_TM=59.637
PRIMER_RIGHT_11_TM=59.989
PRIMER_LEFT_11_GC_PERCENT=45.455
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.7000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=827
PRIMER_PAIR_12_PENALTY=2.425074
PRIMER_LEFT_12_PENALTY=1.713283
PRIMER_RIGHT_12_PENALTY=0.711791
PRIMER_LEFT_12_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_12_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_12=850,21
PRIMER_RIGHT_12=1675,20
PRIMER_LEFT_12_TM=59.287
PRIMER_RIGHT_12_TM=59.288
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=826
PRIMER_PAIR_13_PENALTY=2.495024
PRIMER_LEFT_13_PENALTY=2.362762
PRIMER_RIGHT_13_PENALTY=0.132262
PRIMER_LEFT_13_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_13_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_13=848,22
PRIMER_RIGHT_13=1714,20
PRIMER_LEFT_13_TM=59.637
PRIMER_RIGHT_13_TM=60.132
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.7000
PRIMER_RIGHT_13_END_STABILITY=8.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=867
PRIMER_PAIR_14_PENALTY=2.499206
PRIMER_LEFT_14_PENALTY=1.713283
PRIMER_RIGHT_14_PENALTY=0.785923
PRIMER_LEFT_14_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_14_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_14=850,21
PRIMER_RIGHT_14=1700,20
PRIMER_LEFT_14_TM=59.287
PRIMER_RIGHT_14_TM=60.786
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.8000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=851
PRIMER_PAIR_15_PENALTY=2.514912
PRIMER_LEFT_15_PENALTY=1.713283
PRIMER_RIGHT_15_PENALTY=0.801629
PRIMER_LEFT_15_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_15_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_15=850,21
PRIMER_RIGHT_15=1716,20
PRIMER_LEFT_15_TM=59.287
PRIMER_RIGHT_15_TM=59.198
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=11.1000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=867
PRIMER_PAIR_16_PENALTY=2.648011
PRIMER_LEFT_16_PENALTY=2.637466
PRIMER_RIGHT_16_PENALTY=0.010545
PRIMER_LEFT_16_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_16_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_16=849,22
PRIMER_RIGHT_16=1674,20
PRIMER_LEFT_16_TM=60.637
PRIMER_RIGHT_16_TM=59.989
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=826
PRIMER_PAIR_17_PENALTY=2.665132
PRIMER_LEFT_17_PENALTY=0.327117
PRIMER_RIGHT_17_PENALTY=2.338015
PRIMER_LEFT_17_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_17_SEQUENCE=AACTCACACATACTTGGCTGGA
PRIMER_LEFT_17=856,20
PRIMER_RIGHT_17=1479,22
PRIMER_LEFT_17_TM=59.673
PRIMER_RIGHT_17_TM=59.662
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=624
PRIMER_PAIR_18_PENALTY=2.689881
PRIMER_LEFT_18_PENALTY=0.327117
PRIMER_RIGHT_18_PENALTY=2.362764
PRIMER_LEFT_18_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_18_SEQUENCE=CACATACTTGGCTGGATTTGA
PRIMER_LEFT_18=856,20
PRIMER_RIGHT_18=1473,21
PRIMER_LEFT_18_TM=59.673
PRIMER_RIGHT_18_TM=58.637
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=618
PRIMER_PAIR_19_PENALTY=2.711413
PRIMER_LEFT_19_PENALTY=0.327117
PRIMER_RIGHT_19_PENALTY=2.384296
PRIMER_LEFT_19_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_19_SEQUENCE=AAGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_19=856,20
PRIMER_RIGHT_19=1715,21
PRIMER_LEFT_19_TM=59.673
PRIMER_RIGHT_19_TM=61.384
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=860
PRIMER_PAIR_20_PENALTY=2.729760
PRIMER_LEFT_20_PENALTY=2.719215
PRIMER_RIGHT_20_PENALTY=0.010545
PRIMER_LEFT_20_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_20_SEQUENCE=TGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_20=850,20
PRIMER_RIGHT_20=1674,20
PRIMER_LEFT_20_TM=57.281
PRIMER_RIGHT_20_TM=59.989
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.7000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=825
PRIMER_PAIR_21_PENALTY=2.746843
PRIMER_LEFT_21_PENALTY=0.327117
PRIMER_RIGHT_21_PENALTY=2.419726
PRIMER_LEFT_21_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_21_SEQUENCE=CACACATACTTGGCTGGATTTG
PRIMER_LEFT_21=856,20
PRIMER_RIGHT_21=1475,22
PRIMER_LEFT_21_TM=59.673
PRIMER_RIGHT_21_TM=60.420
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=620
PRIMER_PAIR_22_PENALTY=2.769728
PRIMER_LEFT_22_PENALTY=2.637466
PRIMER_RIGHT_22_PENALTY=0.132262
PRIMER_LEFT_22_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_22_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_22=849,22
PRIMER_RIGHT_22=1714,20
PRIMER_LEFT_22_TM=60.637
PRIMER_RIGHT_22_TM=60.132
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=866
PRIMER_PAIR_23_PENALTY=2.828341
PRIMER_LEFT_23_PENALTY=0.327117
PRIMER_RIGHT_23_PENALTY=2.501224
PRIMER_LEFT_23_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_23_SEQUENCE=ACACATACTTGGCTGGATTTGA
PRIMER_LEFT_23=856,20
PRIMER_RIGHT_23=1474,22
PRIMER_LEFT_23_TM=59.673
PRIMER_RIGHT_23_TM=59.499
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=619
PRIMER_PAIR_24_PENALTY=2.828341
PRIMER_LEFT_24_PENALTY=0.327117
PRIMER_RIGHT_24_PENALTY=2.501224
PRIMER_LEFT_24_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_24_SEQUENCE=TCACACATACTTGGCTGGATTT
PRIMER_LEFT_24=856,20
PRIMER_RIGHT_24=1476,22
PRIMER_LEFT_24_TM=59.673
PRIMER_RIGHT_24_TM=59.499
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=621
PRIMER_PAIR_25_PENALTY=2.851477
PRIMER_LEFT_25_PENALTY=2.719215
PRIMER_RIGHT_25_PENALTY=0.132262
PRIMER_LEFT_25_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_25_SEQUENCE=AGAGTTATTCGGTGGCGTTG
PRIMER_LEFT_25=850,20
PRIMER_RIGHT_25=1714,20
PRIMER_LEFT_25_TM=57.281
PRIMER_RIGHT_25_TM=60.132
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.7000
PRIMER_RIGHT_25_END_STABILITY=8.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=865
PRIMER_PAIR_26_PENALTY=2.926981
PRIMER_LEFT_26_PENALTY=2.215190
PRIMER_RIGHT_26_PENALTY=0.711791
PRIMER_LEFT_26_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_26_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_26=849,21
PRIMER_RIGHT_26=1675,20
PRIMER_LEFT_26_TM=58.785
PRIMER_RIGHT_26_TM=59.288
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.7000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=827
PRIMER_PAIR_27_PENALTY=2.974509
PRIMER_LEFT_27_PENALTY=1.713283
PRIMER_RIGHT_27_PENALTY=1.261226
PRIMER_LEFT_27_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_27_SEQUENCE=ATGTTGCTTTCTCCTCCGTCT
PRIMER_LEFT_27=850,21
PRIMER_RIGHT_27=1675,21
PRIMER_LEFT_27_TM=59.287
PRIMER_RIGHT_27_TM=60.261
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=8.1000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=826
PRIMER_PAIR_28_PENALTY=2.993037
PRIMER_LEFT_28_PENALTY=1.713283
PRIMER_RIGHT_28_PENALTY=1.279754
PRIMER_LEFT_28_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_28_SEQUENCE=AAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_28=850,21
PRIMER_RIGHT_28=1715,20
PRIMER_LEFT_28_TM=59.287
PRIMER_RIGHT_28_TM=58.720
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=9.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=866
PRIMER_PAIR_29_PENALTY=3.001113
PRIMER_LEFT_29_PENALTY=2.215190
PRIMER_RIGHT_29_PENALTY=0.785923
PRIMER_LEFT_29_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_29_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_29=849,21
PRIMER_RIGHT_29=1700,20
PRIMER_LEFT_29_TM=58.785
PRIMER_RIGHT_29_TM=60.786
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.7000
PRIMER_RIGHT_29_END_STABILITY=7.8000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=852
PRIMER_PAIR_30_PENALTY=3.011470
PRIMER_LEFT_30_PENALTY=0.327117
PRIMER_RIGHT_30_PENALTY=2.684353
PRIMER_LEFT_30_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_30_SEQUENCE=GAGTTATTCGGTGGCGTTGG
PRIMER_LEFT_30=856,20
PRIMER_RIGHT_30=1713,20
PRIMER_LEFT_30_TM=59.673
PRIMER_RIGHT_30_TM=62.684
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=858
PRIMER_PAIR_31_PENALTY=3.016819
PRIMER_LEFT_31_PENALTY=2.215190
PRIMER_RIGHT_31_PENALTY=0.801629
PRIMER_LEFT_31_SEQUENCE=TTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_31_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_31=849,21
PRIMER_RIGHT_31=1716,20
PRIMER_LEFT_31_TM=58.785
PRIMER_RIGHT_31_TM=59.198
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.7000
PRIMER_RIGHT_31_END_STABILITY=11.1000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=868
PRIMER_PAIR_32_PENALTY=3.058815
PRIMER_LEFT_32_PENALTY=0.327117
PRIMER_RIGHT_32_PENALTY=2.731698
PRIMER_LEFT_32_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_32_SEQUENCE=GTCGAAGAGTTATTCGGTGG
PRIMER_LEFT_32=856,20
PRIMER_RIGHT_32=1719,20
PRIMER_LEFT_32_TM=59.673
PRIMER_RIGHT_32_TM=57.268
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=864
PRIMER_PAIR_33_PENALTY=3.074553
PRIMER_LEFT_33_PENALTY=2.362762
PRIMER_RIGHT_33_PENALTY=0.711791
PRIMER_LEFT_33_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_33_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_33=848,22
PRIMER_RIGHT_33=1675,20
PRIMER_LEFT_33_TM=59.637
PRIMER_RIGHT_33_TM=59.288
PRIMER_LEFT_33_GC_PERCENT=45.455
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.7000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=828
PRIMER_PAIR_34_PENALTY=3.087787
PRIMER_LEFT_34_PENALTY=0.327117
PRIMER_RIGHT_34_PENALTY=2.760670
PRIMER_LEFT_34_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_34_SEQUENCE=CTCACACATACTTGGCTGGA
PRIMER_LEFT_34=856,20
PRIMER_RIGHT_34=1477,20
PRIMER_LEFT_34_TM=59.673
PRIMER_RIGHT_34_TM=57.239
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=622
PRIMER_PAIR_35_PENALTY=3.097729
PRIMER_LEFT_35_PENALTY=0.327117
PRIMER_RIGHT_35_PENALTY=2.770612
PRIMER_LEFT_35_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_35_SEQUENCE=ACTCACACATACTTGGCTGGA
PRIMER_LEFT_35=856,20
PRIMER_RIGHT_35=1478,21
PRIMER_LEFT_35_TM=59.673
PRIMER_RIGHT_35_TM=58.229
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=623
PRIMER_PAIR_36_PENALTY=3.143647
PRIMER_LEFT_36_PENALTY=0.327117
PRIMER_RIGHT_36_PENALTY=2.816530
PRIMER_LEFT_36_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_36_SEQUENCE=TGGCGTTGGTGATCGTTATG
PRIMER_LEFT_36=856,20
PRIMER_RIGHT_36=1702,20
PRIMER_LEFT_36_TM=59.673
PRIMER_RIGHT_36_TM=62.817
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=847
PRIMER_PAIR_37_PENALTY=3.148686
PRIMER_LEFT_37_PENALTY=2.362762
PRIMER_RIGHT_37_PENALTY=0.785923
PRIMER_LEFT_37_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_37_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_37=848,22
PRIMER_RIGHT_37=1700,20
PRIMER_LEFT_37_TM=59.637
PRIMER_RIGHT_37_TM=60.786
PRIMER_LEFT_37_GC_PERCENT=45.455
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.7000
PRIMER_RIGHT_37_END_STABILITY=7.8000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=853
PRIMER_PAIR_38_PENALTY=3.164391
PRIMER_LEFT_38_PENALTY=2.362762
PRIMER_RIGHT_38_PENALTY=0.801629
PRIMER_LEFT_38_SEQUENCE=GTTGAGAATACATGAGCCGTTG
PRIMER_RIGHT_38_SEQUENCE=GAAGAGTTATTCGGTGGCGT
PRIMER_LEFT_38=848,22
PRIMER_RIGHT_38=1716,20
PRIMER_LEFT_38_TM=59.637
PRIMER_RIGHT_38_TM=59.198
PRIMER_LEFT_38_GC_PERCENT=45.455
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.7000
PRIMER_RIGHT_38_END_STABILITY=11.1000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=869
PRIMER_PAIR_39_PENALTY=3.202002
PRIMER_LEFT_39_PENALTY=0.327117
PRIMER_RIGHT_39_PENALTY=2.874885
PRIMER_LEFT_39_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_39_SEQUENCE=CTCACACATACTTGGCTGGATT
PRIMER_LEFT_39=856,20
PRIMER_RIGHT_39=1477,22
PRIMER_LEFT_39_TM=59.673
PRIMER_RIGHT_39_TM=59.125
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=622
PRIMER_PAIR_40_PENALTY=3.210158
PRIMER_LEFT_40_PENALTY=1.713283
PRIMER_RIGHT_40_PENALTY=1.496875
PRIMER_LEFT_40_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_40_SEQUENCE=GAAGAGTTATTCGGTGGCGTT
PRIMER_LEFT_40=850,21
PRIMER_RIGHT_40=1716,21
PRIMER_LEFT_40_TM=59.287
PRIMER_RIGHT_40_TM=60.497
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=9.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=867
PRIMER_PAIR_41_PENALTY=3.237096
PRIMER_LEFT_41_PENALTY=0.327117
PRIMER_RIGHT_41_PENALTY=2.909980
PRIMER_LEFT_41_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_41_SEQUENCE=TCACACATACTTGGCTGGATT
PRIMER_LEFT_41=856,20
PRIMER_RIGHT_41=1476,21
PRIMER_LEFT_41_TM=59.673
PRIMER_RIGHT_41_TM=58.090
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=621
PRIMER_PAIR_42_PENALTY=3.349257
PRIMER_LEFT_42_PENALTY=2.637466
PRIMER_RIGHT_42_PENALTY=0.711791
PRIMER_LEFT_42_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_42_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_42=849,22
PRIMER_RIGHT_42=1675,20
PRIMER_LEFT_42_TM=60.637
PRIMER_RIGHT_42_TM=59.288
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=827
PRIMER_PAIR_43_PENALTY=3.361056
PRIMER_LEFT_43_PENALTY=1.713283
PRIMER_RIGHT_43_PENALTY=1.647773
PRIMER_LEFT_43_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_43_SEQUENCE=CGTTGGTGATCGTTATGGTT
PRIMER_LEFT_43=850,21
PRIMER_RIGHT_43=1699,20
PRIMER_LEFT_43_TM=59.287
PRIMER_RIGHT_43_TM=58.352
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=850
PRIMER_PAIR_44_PENALTY=3.362235
PRIMER_LEFT_44_PENALTY=0.327117
PRIMER_RIGHT_44_PENALTY=3.035118
PRIMER_LEFT_44_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_44_SEQUENCE=GCGTTGGTGATCGTTATGGTT
PRIMER_LEFT_44=856,20
PRIMER_RIGHT_44=1700,21
PRIMER_LEFT_44_TM=59.673
PRIMER_RIGHT_44_TM=62.035
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=845
PRIMER_PAIR_45_PENALTY=3.381941
PRIMER_LEFT_45_PENALTY=1.713283
PRIMER_RIGHT_45_PENALTY=1.668658
PRIMER_LEFT_45_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_45_SEQUENCE=AGTTATTCGGTGGCGTTGGT
PRIMER_LEFT_45=850,21
PRIMER_RIGHT_45=1712,20
PRIMER_LEFT_45_TM=59.287
PRIMER_RIGHT_45_TM=61.669
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=863
PRIMER_PAIR_46_PENALTY=3.384962
PRIMER_LEFT_46_PENALTY=0.327117
PRIMER_RIGHT_46_PENALTY=3.057845
PRIMER_LEFT_46_SEQUENCE=TACATGAGCCGTTGATGGAG
PRIMER_RIGHT_46_SEQUENCE=ACTCACACATACTTGGCTGGATT
PRIMER_LEFT_46=856,20
PRIMER_RIGHT_46=1478,23
PRIMER_LEFT_46_TM=59.673
PRIMER_RIGHT_46_TM=59.942
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=43.478
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=623
PRIMER_PAIR_47_PENALTY=3.387251
PRIMER_LEFT_47_PENALTY=1.713283
PRIMER_RIGHT_47_PENALTY=1.673968
PRIMER_LEFT_47_SEQUENCE=TGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_47_SEQUENCE=TATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_47=850,21
PRIMER_RIGHT_47=1676,21
PRIMER_LEFT_47_TM=59.287
PRIMER_RIGHT_47_TM=59.326
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=9.6000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=827
PRIMER_PAIR_48_PENALTY=3.423389
PRIMER_LEFT_48_PENALTY=2.637466
PRIMER_RIGHT_48_PENALTY=0.785923
PRIMER_LEFT_48_SEQUENCE=TTGAGAATACATGAGCCGTTGA
PRIMER_RIGHT_48_SEQUENCE=GCGTTGGTGATCGTTATGGT
PRIMER_LEFT_48=849,22
PRIMER_RIGHT_48=1700,20
PRIMER_LEFT_48_TM=60.637
PRIMER_RIGHT_48_TM=60.786
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=7.8000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=852
PRIMER_PAIR_49_PENALTY=3.431006
PRIMER_LEFT_49_PENALTY=2.719215
PRIMER_RIGHT_49_PENALTY=0.711791
PRIMER_LEFT_49_SEQUENCE=TGAGAATACATGAGCCGTTG
PRIMER_RIGHT_49_SEQUENCE=ATGTTGCTTTCTCCTCCGTC
PRIMER_LEFT_49=850,20
PRIMER_RIGHT_49=1675,20
PRIMER_LEFT_49_TM=57.281
PRIMER_RIGHT_49_TM=59.288
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.7000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=826
=
SEQUENCE_ID=MAGIC.105_4_122883_123599_tsu-0_zu-0_122497_2879_TRUE
SEQUENCE_TARGET=950,816
SEQUENCE_TEMPLATE=AGAAGCTTCGCCTTTCTTCTCACCTTTATATTAAGTCATGCATGCTAATGTAGATGAAGNTTGTGGACGCTAAGAAATGTGAGTTGTTGTTGGNGNAGATGGATGTTTTGAAGGAGGTGACTTCGGTGCTTGCTCAAACCAACTAAGAAGAAGAAGAGGAAGCTATTGGATTGTATTATAGGTTTATCGGTTTTTAATCTCGGGTTTNAATGGAGATGTAATGCCTTCTTTTGTTATCTAACTTAGGATTCGTCTCTCTGTCTACGTTAATGAACTTTTAGGACTTTGGATTCTCGTGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAATTAGCTAAGTTGTNTAACAAGCCTTTTGGTCGATGNNTGTAATGAACTTTTAGGACTTTGTAAANGTGCTTCCTTTNTGAATGTCTCTCTACTCAAAACTCATAACCAGAGTTATTACAAAACNAAGAAACACACAATANAATGGATTCGCAAACATATTCAGCAATGACAAGAGTTCTAAATAGCTTATNTGGTGATAGGTTCAANCTCATTAACAAAACGGGTTATGATCAAATACTCGAAGCCTTTTCCCTGCGGANNNNNCANATTGTAACAGTTTCTGTTGCCTTGAGGGAAGCTCCTAANGTTTTGAAACTGTTTCTCAAGAGAACTTAGTTTGAAACAATGCCTTTTCCAAACCAGTTTCAATGGTGTTGGAAAGAAAAATTTGTATGTNCTCAACAAGAAATGATCCATTTCATTGATACGTTTTNATCGGTTGCAGAGATGGTANGAGTGAAGATACTTCCCGGCCTTCTTCCAAATCTTCCTTATGACTTACCCACTCACTCACATCACGTCCNANNAAGAGTTTCAAGTTTTGTATTAANAAAAAGGGAATAAACTAAACAGCAAAAGTTGAGCTNAACTAATTGATCATGCACTGCAAACCACATCAAACGAGCCATTTTCTGCTTAACATATTGAATTGATCNTTTCNTGTACATAGGAATTGTTAGAANCCTATAAATACNNNNNNNNNNNNNNNNNNCNAGAAGGAAANAAATAAAAGCAAGNCCAATATGAAACATTTTTCTCTTGAAACCTAACAAGTCATCACGATTTTCTGTAATTTCCCAAAAACACAGANTTNTGGATCCATCTAGCTANNTAGCTTCCGGATCAGTATTCCTTGTCTCTACAAAATGATCACTCACAATGTAGAATTGTCCCAGGTGGTACCTANCGACACACCGTGCTTCTTTATGATCTTCACCGCCTCAAGAAGGACCTCTTGTTGTGTATTGTATAGCTCNTCTTTTCCCTGAGANAAATGNCCACCANTCTAATGTAAAAAGATCACATCAAGCTACNAAACTTTGTCTACATTTGACGNCAAAGATCCCTAGCAGTAGTAGTGGTAGTNGTATCATTCAAAAANTGAGAAGACTGTNACAGANACTAAAGAGAGAGTGTACCATGCGTATCAGNTTGCACCCAATGGTAAGTTCAGCGAAAGATTTTTCCACTCGCGATAAGTAACAGTGTGGAGCTTCTTTTCCTAAGAAAACTTTTNTGTTNGACCTCAACATTTCCTTNATTTCATTTGATATTTGAGGAATCANATCCAAGTCATCGATCTGCAGTGGGATCTTGGATGCTATTGCTCGCCACTGAGCACGTGACTTATTCACAATCACCTATATATGTNNNNNNNCTNCANNGTCNGATTCAAACANATGAGATAANTAAATNATTGNGAGTGTTAACTCAGTGTGGNATTNTGAAAGCTAGAGCATTAGCTTCTGCAATANGAAGATGAATAGTGNGAANCAGATAAACTAACCTGACTCGAAAACAGNGAATTNGGTACCAAAACCGGAAATTTTTCNGCATTCAACAAAGATGTTGTCGTAAGTCCCATTTCAATAACTTGACCTTCTACGGATCCAGCCTGCAAAGTGGGGACAAGGAAGACACTATATCTTGAGAAAAATATGAGTGAGAACTGCCAATANATTTGCTTGCTCTATGANACTAAATAAAAGAAGAGAGCNNNNNAGAAAAAAANGTTCANCTAACTTTGATAGTATCACCCATAGAGAAAGGCCGCGAGAATTGCATTGACAGACCACTCAGCACATTACCAAGGATATCTCTGGCAGCAAATGCGGTGGCTACCCCTGAAAACGCAAATCAAAATTGGTATGTTATGAGCAAATACTATAGAATCAGTANNNNNNCTAANAATGTTAGCAACCCCTGAAAAAGATAAGACTATATGCTTGTGNTGGCCTCTAGAGGCCTTTATTTTCTGNTGAATCCTAGCAANTAGCATTATANGCNGAGCAAAACNACTAATATGAAATGGAGAACATTAGACAGACCTCCAACTCCACCAACTGTCAAGATAGATTGAACAGCTACACCACATGCTTCAGCAGAAGCCATCAAACCGATAGCGAAAAGACCAACAGATGATACTTTGTCTAAAGTCAATACCTTNTCCCTATCAAGCCCACCAAAACTCTTAGCCGACAACATTCGGGTAATCACATTGGTCTTCCACCGATANAGGAACCACACAAGTGAGAGAATAACCGCTCCCTTCACNGTCGGGGAAAAGTATTGAGCTGCTATAGTTGTTGGCGCCACCATTGCAGCACTGCAGCATATCACATTCATCAAGTCTATCTATTTTCTACAACATACATG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_9014795_9014802_kn-0_wil-2_3567882_2092_TRUE
SEQUENCE_TARGET=950,107
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_6121798_6122288_ler-0_oy-0_3087_25003_TRUE
SEQUENCE_TARGET=950,590
SEQUENCE_TEMPLATE=GCATCAACANNNNNTGTGGTGTGGTGGGGTTAAAGTTTGAATCTCTCTTTAATTTAAACTATTAAAACAAACTTAAATTATTGGANTTTCGTACNATGACTNTCAATCAAATGTTTTAGAATTAGACACGGTTTTCGAAAGTGGTTTTCCCTCGTTGAATTTGTCAACAGTATCAGATTCTACATTGTTGGTTACTAATCTTTTNCTTGAAGTAGGTGTTGAATTAATCCTCTGTTGTTTATGNNAGGAGATCTCGAGACATTTATGGTTAACAGTTANNACTNCATGTTTGACTTTAAACTGATTNTNTTTTNTTCTTNTTCTTNTGTAGCTCCCAACAGAGGAATGAACTGGTCTTTCTCAAACAAAGTATCAGCNNCTTCTTCTCAGTTTCTATCNTTCAGGCCAACTCAAGAANATAGACATAGAAAGNCTGGAAATTATCATCTTCCTCACTCTGGTTCCTTCATGNCATCATCAGTAGCTGATGTTTATGATTCAACCCGCAAAGCTCCTTANAGTTCTGTACAGGTATTTGTCATCAAAACCTATNTTAACCAAGACCNTTGTGTNTTTTNTNTCCTTNGCAAGATAGCTTTANAANTNNNNNCTGTTTTATGAGCNTATAGNAATTGNTTTTGNGTCTAGTTTAGCACAAGTTCATGGCAATTAGTTTGTGGATCTAANCTTGGTTTAATANTGATTCATTTTAAGTGTNAGCTAAGCTTCTCATTTTTGATAAGTTANTTCATACANTGCCTCACACCTACNTTATGGCTTGTTACTCTNAGGGAGTGNGGATGTTCCCTAATTCCAATCAACACGAAGAAACTAACNCAGTTTCCATGTCNATGCCGGGTTTCCAGTCTCATCATTATGCACCAGGAGGAAGAAGCTTCATGAACAATAACAATAACTCACAACCTTTGGTAGGAGTTCCTATCATGGCACCTCCAATTTCAATCCTTCCTCCTCCAGGTTCCATTGTAGGGACNACTGANATTAGGTACNCACTNGTCATCATATCATACAGANACTCTTTCTACATTTTCATAGTTGACTAAAGACTTATTTTTGTCAGATCTTCTTCCAAGCCAATAGGTTCACCTGCGCAGTTGACGATCTTTTATGCCGGTTCAGTTTGTGTTTACGATGACATATCTCCTGAAAAGGTATCTCAATCATTTNCTTCCATNTATGCATCTCTTTTACTCGTAAGGTATGGTACTCATTTGCNTTCTTTCATTTCTCAGGCAAAGGCGATAATGTTGCTAGCTGGGAACGGTTCCTCTATGCCTCAAGTCTTTTCGCCGCCTCAAACTCATCAACAAGTNGTCCATCATACTCGTGCCTCTGTNGATTCTTCAGCTATGCCTCCTAGCTTCATGCCTACAATATCTTATCTNAGCCCTGAAGCTGGAAGTAGCACAAACGGACTCGGAGCNACAANAGCNACAAGAGGCTTGACGTCAACATATCACAACAACCAANCTAATGGATCCAATATTAACTGCCCAGTACCAGTTTCTTGTTCTACCAATGTAATGGCTCCAACAGGTAAAAAACAAAGTCAGAGACCTGATACTACATTCGCCATCTANCTTACTAGTATTTTCATGGATGTAACTTCATTCTCGTTCTGTTTCTTATGCAGTGGCATTACCTCTGGCTCGCAAAGCATCCCTGGCTAGGTTTTTAGAGAAACGCAAAGAAAGGTACGCAACACTTCTTTAGAATACACCATTCAATAGTTTCTTGNGCTAACTCTCTTTCTCGCTGTGGGTTTNTCAGGGTCACGAGCGTATCCCCATATTGCTTAGACAAGAAGTCATCGACAGATTGTCGCAGATCAATGTCTGAATGCATTAGTTCTTCTCTCAGCTCTGCAACNNAATTTCATCNACAGTAAGAAGGTTGCTTTAGACCACTCCACATCCATATTTGCNTTTCAATGGCGGTCTTTTCAATGTCTCAGTTAATTTTTNCTCACTCGCNACACTGAGTTTCTNCTTAGCTTTATATATACGATAGTGTATACTTTGTTTACATGTTTTTTGGTGGAATGGAACTTATGAGAGCATATCAGATNTGTACTTGGGAAAATTAGTAGAAACTGTTTGTTTNNTTTNTTTTAACTCTNTTCTTTTGTATATATCACTGAAGCTCGCATATGTATAATTCATGNAATGNAATTGCNTCGCTTCTGTTTCCCTAANTTATTTCTCTAATCAATTTNCTATNNAAATATGGGGANTTTTTTNNNAGGATTTGCTAATTTGTANTCTGGTTAACAANAAGNTTGATACATTNCCANTTACCAACTCGAATTCACAAAANAAGTCATCATTANATATGGAGAAAATGGNACATCTTTTAGAATTTGCTTTAATTCGTTTTGGNTAATTATTNTTCGGTCTTCGTTTNGGCTGTGAGTTTCTTTGTAACATCCNAATNTNAATATGTGGGCCATTTTGTGTTGTAGCCCATCAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.935301
PRIMER_LEFT_0_PENALTY=0.924755
PRIMER_RIGHT_0_PENALTY=0.010545
PRIMER_LEFT_0_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_0_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_0=871,20
PRIMER_RIGHT_0=1784,20
PRIMER_LEFT_0_TM=59.075
PRIMER_RIGHT_0_TM=59.989
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=914
PRIMER_PAIR_1_PENALTY=1.020330
PRIMER_LEFT_1_PENALTY=0.924755
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_1_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_1=871,20
PRIMER_RIGHT_1=1693,20
PRIMER_LEFT_1_TM=59.075
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=823
PRIMER_PAIR_2_PENALTY=1.020330
PRIMER_LEFT_2_PENALTY=0.924755
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_2_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_2=871,20
PRIMER_RIGHT_2=1695,20
PRIMER_LEFT_2_TM=59.075
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=825
PRIMER_PAIR_3_PENALTY=1.091582
PRIMER_LEFT_3_PENALTY=1.081037
PRIMER_RIGHT_3_PENALTY=0.010545
PRIMER_LEFT_3_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_3_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_3=872,21
PRIMER_RIGHT_3=1784,20
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=59.989
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=913
PRIMER_PAIR_4_PENALTY=1.091582
PRIMER_LEFT_4_PENALTY=1.081037
PRIMER_RIGHT_4_PENALTY=0.010545
PRIMER_LEFT_4_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_4_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_4=871,21
PRIMER_RIGHT_4=1784,20
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=914
PRIMER_PAIR_5_PENALTY=1.176611
PRIMER_LEFT_5_PENALTY=1.081037
PRIMER_RIGHT_5_PENALTY=0.095574
PRIMER_LEFT_5_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_5_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_5=872,21
PRIMER_RIGHT_5=1693,20
PRIMER_LEFT_5_TM=60.081
PRIMER_RIGHT_5_TM=60.096
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=822
PRIMER_PAIR_6_PENALTY=1.176611
PRIMER_LEFT_6_PENALTY=1.081037
PRIMER_RIGHT_6_PENALTY=0.095574
PRIMER_LEFT_6_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_6_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_6=872,21
PRIMER_RIGHT_6=1695,20
PRIMER_LEFT_6_TM=60.081
PRIMER_RIGHT_6_TM=60.096
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.1000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=824
PRIMER_PAIR_7_PENALTY=1.176611
PRIMER_LEFT_7_PENALTY=1.081037
PRIMER_RIGHT_7_PENALTY=0.095574
PRIMER_LEFT_7_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_7_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_7=871,21
PRIMER_RIGHT_7=1693,20
PRIMER_LEFT_7_TM=60.081
PRIMER_RIGHT_7_TM=60.096
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=823
PRIMER_PAIR_8_PENALTY=1.176611
PRIMER_LEFT_8_PENALTY=1.081037
PRIMER_RIGHT_8_PENALTY=0.095574
PRIMER_LEFT_8_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_8_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_8=871,21
PRIMER_RIGHT_8=1695,20
PRIMER_LEFT_8_TM=60.081
PRIMER_RIGHT_8_TM=60.096
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=825
PRIMER_PAIR_9_PENALTY=1.201716
PRIMER_LEFT_9_PENALTY=1.191170
PRIMER_RIGHT_9_PENALTY=0.010545
PRIMER_LEFT_9_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_9_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_9=805,21
PRIMER_RIGHT_9=1784,20
PRIMER_LEFT_9_TM=59.809
PRIMER_RIGHT_9_TM=59.989
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=980
PRIMER_PAIR_10_PENALTY=1.286745
PRIMER_LEFT_10_PENALTY=1.191170
PRIMER_RIGHT_10_PENALTY=0.095574
PRIMER_LEFT_10_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_10_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_10=805,21
PRIMER_RIGHT_10=1693,20
PRIMER_LEFT_10_TM=59.809
PRIMER_RIGHT_10_TM=60.096
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=889
PRIMER_PAIR_11_PENALTY=1.286745
PRIMER_LEFT_11_PENALTY=1.191170
PRIMER_RIGHT_11_PENALTY=0.095574
PRIMER_LEFT_11_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_11_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_11=805,21
PRIMER_RIGHT_11=1695,20
PRIMER_LEFT_11_TM=59.809
PRIMER_RIGHT_11_TM=60.096
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=891
PRIMER_PAIR_12_PENALTY=1.326797
PRIMER_LEFT_12_PENALTY=0.924755
PRIMER_RIGHT_12_PENALTY=0.402042
PRIMER_LEFT_12_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_12_SEQUENCE=CCCACAGCGAGAAAGAGAGT
PRIMER_LEFT_12=871,20
PRIMER_RIGHT_12=1782,20
PRIMER_LEFT_12_TM=59.075
PRIMER_RIGHT_12_TM=59.598
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.1000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=912
PRIMER_PAIR_13_PENALTY=1.326797
PRIMER_LEFT_13_PENALTY=0.924755
PRIMER_RIGHT_13_PENALTY=0.402042
PRIMER_LEFT_13_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_13_SEQUENCE=ACCCACAGCGAGAAAGAGAG
PRIMER_LEFT_13=871,20
PRIMER_RIGHT_13=1783,20
PRIMER_LEFT_13_TM=59.075
PRIMER_RIGHT_13_TM=59.598
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=913
PRIMER_PAIR_14_PENALTY=1.452217
PRIMER_LEFT_14_PENALTY=0.924755
PRIMER_RIGHT_14_PENALTY=0.527462
PRIMER_LEFT_14_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_14_SEQUENCE=AAACCCACAGCGAGAAAGAG
PRIMER_LEFT_14=871,20
PRIMER_RIGHT_14=1785,20
PRIMER_LEFT_14_TM=59.075
PRIMER_RIGHT_14_TM=59.473
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=915
PRIMER_PAIR_15_PENALTY=1.483079
PRIMER_LEFT_15_PENALTY=1.081037
PRIMER_RIGHT_15_PENALTY=0.402042
PRIMER_LEFT_15_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_15_SEQUENCE=CCCACAGCGAGAAAGAGAGT
PRIMER_LEFT_15=872,21
PRIMER_RIGHT_15=1782,20
PRIMER_LEFT_15_TM=60.081
PRIMER_RIGHT_15_TM=59.598
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.1000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=911
PRIMER_PAIR_16_PENALTY=1.483079
PRIMER_LEFT_16_PENALTY=1.081037
PRIMER_RIGHT_16_PENALTY=0.402042
PRIMER_LEFT_16_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_16_SEQUENCE=ACCCACAGCGAGAAAGAGAG
PRIMER_LEFT_16=872,21
PRIMER_RIGHT_16=1783,20
PRIMER_LEFT_16_TM=60.081
PRIMER_RIGHT_16_TM=59.598
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=2.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=912
PRIMER_PAIR_17_PENALTY=1.483079
PRIMER_LEFT_17_PENALTY=1.081037
PRIMER_RIGHT_17_PENALTY=0.402042
PRIMER_LEFT_17_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_17_SEQUENCE=CCCACAGCGAGAAAGAGAGT
PRIMER_LEFT_17=871,21
PRIMER_RIGHT_17=1782,20
PRIMER_LEFT_17_TM=60.081
PRIMER_RIGHT_17_TM=59.598
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.1000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=912
PRIMER_PAIR_18_PENALTY=1.483079
PRIMER_LEFT_18_PENALTY=1.081037
PRIMER_RIGHT_18_PENALTY=0.402042
PRIMER_LEFT_18_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_18_SEQUENCE=ACCCACAGCGAGAAAGAGAG
PRIMER_LEFT_18=871,21
PRIMER_RIGHT_18=1783,20
PRIMER_LEFT_18_TM=60.081
PRIMER_RIGHT_18_TM=59.598
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=913
PRIMER_PAIR_19_PENALTY=1.518433
PRIMER_LEFT_19_PENALTY=0.924755
PRIMER_RIGHT_19_PENALTY=0.593677
PRIMER_LEFT_19_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_19_SEQUENCE=GCGTACCTTTCTTTGCGTTT
PRIMER_LEFT_19=871,20
PRIMER_RIGHT_19=1720,20
PRIMER_LEFT_19_TM=59.075
PRIMER_RIGHT_19_TM=59.406
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=850
PRIMER_PAIR_20_PENALTY=1.593212
PRIMER_LEFT_20_PENALTY=1.191170
PRIMER_RIGHT_20_PENALTY=0.402042
PRIMER_LEFT_20_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_20_SEQUENCE=CCCACAGCGAGAAAGAGAGT
PRIMER_LEFT_20=805,21
PRIMER_RIGHT_20=1782,20
PRIMER_LEFT_20_TM=59.809
PRIMER_RIGHT_20_TM=59.598
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.4000
PRIMER_RIGHT_20_END_STABILITY=6.1000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=978
PRIMER_PAIR_21_PENALTY=1.593212
PRIMER_LEFT_21_PENALTY=1.191170
PRIMER_RIGHT_21_PENALTY=0.402042
PRIMER_LEFT_21_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_21_SEQUENCE=ACCCACAGCGAGAAAGAGAG
PRIMER_LEFT_21=805,21
PRIMER_RIGHT_21=1783,20
PRIMER_LEFT_21_TM=59.809
PRIMER_RIGHT_21_TM=59.598
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.4000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=979
PRIMER_PAIR_22_PENALTY=1.608499
PRIMER_LEFT_22_PENALTY=1.081037
PRIMER_RIGHT_22_PENALTY=0.527462
PRIMER_LEFT_22_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_22_SEQUENCE=AAACCCACAGCGAGAAAGAG
PRIMER_LEFT_22=872,21
PRIMER_RIGHT_22=1785,20
PRIMER_LEFT_22_TM=60.081
PRIMER_RIGHT_22_TM=59.473
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=914
PRIMER_PAIR_23_PENALTY=1.608499
PRIMER_LEFT_23_PENALTY=1.081037
PRIMER_RIGHT_23_PENALTY=0.527462
PRIMER_LEFT_23_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_23_SEQUENCE=AAACCCACAGCGAGAAAGAG
PRIMER_LEFT_23=871,21
PRIMER_RIGHT_23=1785,20
PRIMER_LEFT_23_TM=60.081
PRIMER_RIGHT_23_TM=59.473
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=915
PRIMER_PAIR_24_PENALTY=1.609616
PRIMER_LEFT_24_PENALTY=1.599071
PRIMER_RIGHT_24_PENALTY=0.010545
PRIMER_LEFT_24_SEQUENCE=ATCATTATGCACCAGGAGGAA
PRIMER_RIGHT_24_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_24=870,21
PRIMER_RIGHT_24=1784,20
PRIMER_LEFT_24_TM=59.401
PRIMER_RIGHT_24_TM=59.989
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=915
PRIMER_PAIR_25_PENALTY=1.674714
PRIMER_LEFT_25_PENALTY=1.081037
PRIMER_RIGHT_25_PENALTY=0.593677
PRIMER_LEFT_25_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_25_SEQUENCE=GCGTACCTTTCTTTGCGTTT
PRIMER_LEFT_25=872,21
PRIMER_RIGHT_25=1720,20
PRIMER_LEFT_25_TM=60.081
PRIMER_RIGHT_25_TM=59.406
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=8.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=849
PRIMER_PAIR_26_PENALTY=1.674714
PRIMER_LEFT_26_PENALTY=1.081037
PRIMER_RIGHT_26_PENALTY=0.593677
PRIMER_LEFT_26_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_26_SEQUENCE=GCGTACCTTTCTTTGCGTTT
PRIMER_LEFT_26=871,21
PRIMER_RIGHT_26=1720,20
PRIMER_LEFT_26_TM=60.081
PRIMER_RIGHT_26_TM=59.406
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=850
PRIMER_PAIR_27_PENALTY=1.688828
PRIMER_LEFT_27_PENALTY=1.678283
PRIMER_RIGHT_27_PENALTY=0.010545
PRIMER_LEFT_27_SEQUENCE=TTATGCACCAGGAGGAAGAAG
PRIMER_RIGHT_27_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_27=874,21
PRIMER_RIGHT_27=1784,20
PRIMER_LEFT_27_TM=59.322
PRIMER_RIGHT_27_TM=59.989
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=911
PRIMER_PAIR_28_PENALTY=1.694645
PRIMER_LEFT_28_PENALTY=1.599071
PRIMER_RIGHT_28_PENALTY=0.095574
PRIMER_LEFT_28_SEQUENCE=ATCATTATGCACCAGGAGGAA
PRIMER_RIGHT_28_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_28=870,21
PRIMER_RIGHT_28=1693,20
PRIMER_LEFT_28_TM=59.401
PRIMER_RIGHT_28_TM=60.096
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=824
PRIMER_PAIR_29_PENALTY=1.694645
PRIMER_LEFT_29_PENALTY=1.599071
PRIMER_RIGHT_29_PENALTY=0.095574
PRIMER_LEFT_29_SEQUENCE=ATCATTATGCACCAGGAGGAA
PRIMER_RIGHT_29_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_29=870,21
PRIMER_RIGHT_29=1695,20
PRIMER_LEFT_29_TM=59.401
PRIMER_RIGHT_29_TM=60.096
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=8.1000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=826
PRIMER_PAIR_30_PENALTY=1.712843
PRIMER_LEFT_30_PENALTY=1.702298
PRIMER_RIGHT_30_PENALTY=0.010545
PRIMER_LEFT_30_SEQUENCE=TTATGCACCAGGAGGAAGAA
PRIMER_RIGHT_30_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_30=874,20
PRIMER_RIGHT_30=1784,20
PRIMER_LEFT_30_TM=58.298
PRIMER_RIGHT_30_TM=59.989
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=2.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=911
PRIMER_PAIR_31_PENALTY=1.718632
PRIMER_LEFT_31_PENALTY=1.191170
PRIMER_RIGHT_31_PENALTY=0.527462
PRIMER_LEFT_31_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_31_SEQUENCE=AAACCCACAGCGAGAAAGAG
PRIMER_LEFT_31=805,21
PRIMER_RIGHT_31=1785,20
PRIMER_LEFT_31_TM=59.809
PRIMER_RIGHT_31_TM=59.473
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=981
PRIMER_PAIR_32_PENALTY=1.726525
PRIMER_LEFT_32_PENALTY=0.924755
PRIMER_RIGHT_32_PENALTY=0.801770
PRIMER_LEFT_32_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_32_SEQUENCE=TGCGTACCTTTCTTTGCGTT
PRIMER_LEFT_32=871,20
PRIMER_RIGHT_32=1721,20
PRIMER_LEFT_32_TM=59.075
PRIMER_RIGHT_32_TM=60.802
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=851
PRIMER_PAIR_33_PENALTY=1.726525
PRIMER_LEFT_33_PENALTY=0.924755
PRIMER_RIGHT_33_PENALTY=0.801770
PRIMER_LEFT_33_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_33_SEQUENCE=TTGCGTACCTTTCTTTGCGT
PRIMER_LEFT_33=871,20
PRIMER_RIGHT_33=1722,20
PRIMER_LEFT_33_TM=59.075
PRIMER_RIGHT_33_TM=60.802
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=9.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=852
PRIMER_PAIR_34_PENALTY=1.773857
PRIMER_LEFT_34_PENALTY=1.678283
PRIMER_RIGHT_34_PENALTY=0.095574
PRIMER_LEFT_34_SEQUENCE=TTATGCACCAGGAGGAAGAAG
PRIMER_RIGHT_34_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_34=874,21
PRIMER_RIGHT_34=1693,20
PRIMER_LEFT_34_TM=59.322
PRIMER_RIGHT_34_TM=60.096
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=8.5000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=820
PRIMER_PAIR_35_PENALTY=1.773857
PRIMER_LEFT_35_PENALTY=1.678283
PRIMER_RIGHT_35_PENALTY=0.095574
PRIMER_LEFT_35_SEQUENCE=TTATGCACCAGGAGGAAGAAG
PRIMER_RIGHT_35_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_35=874,21
PRIMER_RIGHT_35=1695,20
PRIMER_LEFT_35_TM=59.322
PRIMER_RIGHT_35_TM=60.096
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=8.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=822
PRIMER_PAIR_36_PENALTY=1.784848
PRIMER_LEFT_36_PENALTY=1.191170
PRIMER_RIGHT_36_PENALTY=0.593677
PRIMER_LEFT_36_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_36_SEQUENCE=GCGTACCTTTCTTTGCGTTT
PRIMER_LEFT_36=805,21
PRIMER_RIGHT_36=1720,20
PRIMER_LEFT_36_TM=59.809
PRIMER_RIGHT_36_TM=59.406
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.4000
PRIMER_RIGHT_36_END_STABILITY=8.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=916
PRIMER_PAIR_37_PENALTY=1.797872
PRIMER_LEFT_37_PENALTY=1.702298
PRIMER_RIGHT_37_PENALTY=0.095574
PRIMER_LEFT_37_SEQUENCE=TTATGCACCAGGAGGAAGAA
PRIMER_RIGHT_37_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_37=874,20
PRIMER_RIGHT_37=1693,20
PRIMER_LEFT_37_TM=58.298
PRIMER_RIGHT_37_TM=60.096
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=820
PRIMER_PAIR_38_PENALTY=1.797872
PRIMER_LEFT_38_PENALTY=1.702298
PRIMER_RIGHT_38_PENALTY=0.095574
PRIMER_LEFT_38_SEQUENCE=TTATGCACCAGGAGGAAGAA
PRIMER_RIGHT_38_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_38=874,20
PRIMER_RIGHT_38=1695,20
PRIMER_LEFT_38_TM=58.298
PRIMER_RIGHT_38_TM=60.096
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=822
PRIMER_PAIR_39_PENALTY=1.837398
PRIMER_LEFT_39_PENALTY=1.826853
PRIMER_RIGHT_39_PENALTY=0.010545
PRIMER_LEFT_39_SEQUENCE=CATTATGCACCAGGAGGAAG
PRIMER_RIGHT_39_SEQUENCE=AACCCACAGCGAGAAAGAGA
PRIMER_LEFT_39=872,20
PRIMER_RIGHT_39=1784,20
PRIMER_LEFT_39_TM=58.173
PRIMER_RIGHT_39_TM=59.989
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=2.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=913
PRIMER_PAIR_40_PENALTY=1.882807
PRIMER_LEFT_40_PENALTY=1.081037
PRIMER_RIGHT_40_PENALTY=0.801770
PRIMER_LEFT_40_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_40_SEQUENCE=TGCGTACCTTTCTTTGCGTT
PRIMER_LEFT_40=872,21
PRIMER_RIGHT_40=1721,20
PRIMER_LEFT_40_TM=60.081
PRIMER_RIGHT_40_TM=60.802
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=9.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=850
PRIMER_PAIR_41_PENALTY=1.882807
PRIMER_LEFT_41_PENALTY=1.081037
PRIMER_RIGHT_41_PENALTY=0.801770
PRIMER_LEFT_41_SEQUENCE=CATTATGCACCAGGAGGAAGA
PRIMER_RIGHT_41_SEQUENCE=TTGCGTACCTTTCTTTGCGT
PRIMER_LEFT_41=872,21
PRIMER_RIGHT_41=1722,20
PRIMER_LEFT_41_TM=60.081
PRIMER_RIGHT_41_TM=60.802
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=9.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=851
PRIMER_PAIR_42_PENALTY=1.882807
PRIMER_LEFT_42_PENALTY=1.081037
PRIMER_RIGHT_42_PENALTY=0.801770
PRIMER_LEFT_42_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_42_SEQUENCE=TGCGTACCTTTCTTTGCGTT
PRIMER_LEFT_42=871,21
PRIMER_RIGHT_42=1721,20
PRIMER_LEFT_42_TM=60.081
PRIMER_RIGHT_42_TM=60.802
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=9.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=851
PRIMER_PAIR_43_PENALTY=1.882807
PRIMER_LEFT_43_PENALTY=1.081037
PRIMER_RIGHT_43_PENALTY=0.801770
PRIMER_LEFT_43_SEQUENCE=TCATTATGCACCAGGAGGAAG
PRIMER_RIGHT_43_SEQUENCE=TTGCGTACCTTTCTTTGCGT
PRIMER_LEFT_43=871,21
PRIMER_RIGHT_43=1722,20
PRIMER_LEFT_43_TM=60.081
PRIMER_RIGHT_43_TM=60.802
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=9.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=852
PRIMER_PAIR_44_PENALTY=1.899192
PRIMER_LEFT_44_PENALTY=0.924755
PRIMER_RIGHT_44_PENALTY=0.974437
PRIMER_LEFT_44_SEQUENCE=TCATTATGCACCAGGAGGAA
PRIMER_RIGHT_44_SEQUENCE=AGCAATATGGGGATACGCTC
PRIMER_LEFT_44=871,20
PRIMER_RIGHT_44=1816,20
PRIMER_LEFT_44_TM=59.075
PRIMER_RIGHT_44_TM=59.026
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=946
PRIMER_PAIR_45_PENALTY=1.922428
PRIMER_LEFT_45_PENALTY=1.826853
PRIMER_RIGHT_45_PENALTY=0.095574
PRIMER_LEFT_45_SEQUENCE=CATTATGCACCAGGAGGAAG
PRIMER_RIGHT_45_SEQUENCE=AACCTAGCCAGGGATGCTTT
PRIMER_LEFT_45=872,20
PRIMER_RIGHT_45=1693,20
PRIMER_LEFT_45_TM=58.173
PRIMER_RIGHT_45_TM=60.096
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=822
PRIMER_PAIR_46_PENALTY=1.922428
PRIMER_LEFT_46_PENALTY=1.826853
PRIMER_RIGHT_46_PENALTY=0.095574
PRIMER_LEFT_46_SEQUENCE=CATTATGCACCAGGAGGAAG
PRIMER_RIGHT_46_SEQUENCE=AAAACCTAGCCAGGGATGCT
PRIMER_LEFT_46=872,20
PRIMER_RIGHT_46=1695,20
PRIMER_LEFT_46_TM=58.173
PRIMER_RIGHT_46_TM=60.096
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=824
PRIMER_PAIR_47_PENALTY=1.992940
PRIMER_LEFT_47_PENALTY=1.191170
PRIMER_RIGHT_47_PENALTY=0.801770
PRIMER_LEFT_47_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_47_SEQUENCE=TGCGTACCTTTCTTTGCGTT
PRIMER_LEFT_47=805,21
PRIMER_RIGHT_47=1721,20
PRIMER_LEFT_47_TM=59.809
PRIMER_RIGHT_47_TM=60.802
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.4000
PRIMER_RIGHT_47_END_STABILITY=9.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=917
PRIMER_PAIR_48_PENALTY=1.992940
PRIMER_LEFT_48_PENALTY=1.191170
PRIMER_RIGHT_48_PENALTY=0.801770
PRIMER_LEFT_48_SEQUENCE=CCCTAATTCCAATCAACACGA
PRIMER_RIGHT_48_SEQUENCE=TTGCGTACCTTTCTTTGCGT
PRIMER_LEFT_48=805,21
PRIMER_RIGHT_48=1722,20
PRIMER_LEFT_48_TM=59.809
PRIMER_RIGHT_48_TM=60.802
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=918
PRIMER_PAIR_49_PENALTY=2.001113
PRIMER_LEFT_49_PENALTY=1.599071
PRIMER_RIGHT_49_PENALTY=0.402042
PRIMER_LEFT_49_SEQUENCE=ATCATTATGCACCAGGAGGAA
PRIMER_RIGHT_49_SEQUENCE=CCCACAGCGAGAAAGAGAGT
PRIMER_LEFT_49=870,21
PRIMER_RIGHT_49=1782,20
PRIMER_LEFT_49_TM=59.401
PRIMER_RIGHT_49_TM=59.598
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.1000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=913
=
SEQUENCE_ID=MAGIC.105_4_7410423_7410500_hi-0_po-0_57100_52515_TRUE
SEQUENCE_TARGET=950,177
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAANATTTGAATACTAGACNNTTTTNTNTTNTTTTTTTGACAANAAATAAAATGGANTATGAAACGTCTTTCATTTAAANANNNNAAGTTACAACAAAAACATATATGCGCGNAAAAAGATAACGNNTCACACTTAAACAAAAGATTGAAACATTNANCCCTTAACNTGCATACATGCATGCAATTATTCAAACTGTGATTATTTTAAAGAGTAAACTGTGTGCGTCGTATGNGTAATTGATATCCCTCAAGCACATTGGAAATTAGACGGAAGCCTTCTGTTGCAAACGTTNAGAAGAACACTGAGAGATATAGGAACGTTAAGGTTGATGCCAAGAACGTTAGCCCTAAGAGCAGTGCATAGGCAAANCGCAGCGTCAAGGTCAACCAAACCTTGNATGAGTGAGCAGCATGGTTGAGNTGATGGCTGTCCCAANTGCACGTTAAGTAGACCNCTTAGNACATTCGCACATACACCNAGTCTGAGAGCATCGATAGGACAGTTTCCGGATGAACCAGGGGTTCTCGGAGGAATGACTGGCGTAGGGTTAGGACTTGGTACNGNAGGANTTGGGACCGAAGGAGTTGGNACNGAAGGANTTGGGACCGAAGGAGTTGGAACATNNNNNNNNNNNNNNNAAGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGGAGCGGCCTAGGCTTAGGACTNGGGTTGCATCTACAATNAGTANCAGCGGTTAAGGTGAANAAAAGGATGTTGAGNGCAAAGAAAAGAGCAAGAGAGGCTGAGTTCTTTGAAGCCATTGTTATNNNNNNTGTTTGAGTGANANATGANGAANATGAAGAGANGGTGTGGGTATTTATAGGATTTTAGAAGGATGNNNNNNNNTNAAGAGGACCAACTTGTTTTACTTACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGTAACGCAAAATCATTTGATTTCCCGGAAAATNCAGATTTAATGTTTCTTAAAGTCANNAGTAATATTTTTGTCCCGAAAGAAAAATCTCATNTTTTTAGCAATGCTAGGACNAANANNNNNNNNNNNNNNNATAGAGCTAATATTTGCACCGAAAGAANATATAATCGTTTCAGCAATGCGAAGACAAAAAGAAAAACNAAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_8561977_8562148_wil-2_ct-1_9760_4406_TRUE
SEQUENCE_TARGET=950,271
SEQUENCE_TEMPLATE=TAACNNGTTTGACATATATATTTCAAAANCCACTAACNAAAATATTAATAAATACTAGTTTTTCGAAGNNAANAAAAAAACGTTTGCATTATTTAGTCTTGAAACAACNAATTNAAAAGGAAAGGTTGTCGGTGAACAAATTTGAAGGCCACTNGAATAATGCCTTNTATTTTTGTCAGAAGNTTACATTGNCTAGGTACTTGTTTAACTTAATTCNTACAATTTCTGATACCAATTGTGTAATATCACTGAGTTAGTTGTTNNTGTTGTTTCATTANCCAAAAAACAAAAGTTCTACACGTTGGCATNCCTTTCTTTTGGTCATATATGAGTTTGATAACAATATATAAACTTCAAAATCAGGATTTTCTTTGCTAAATCGAAAGAGTTTCGTANAANATTATTGATTGTTTTAAGTTTAATTAAGTAACTTGTAGGTTTAATTCAAAAACTTGTAGGTATCTCNGTGATATTANTGGTTTATGAAANNNNNTATTAANGCTTGTNTCGANTTTGTNTTNGGCCGGCCTTTCTTANCAGAATTGAACCTCAGANAGAAACTGTAATAAACCAAAGATACTGGACATGTNTATGTCGGCCTTTGGNGTCGTCTGTAACTAAAAANAAACTGGATANTTATCCNNGATATTACTAATCNTNTGAAATCTTNTTGTAATTATCTATAGATTNTTAGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTTGAATGTAAAATCCANNNTTGGATAGTATCCAATCNGTAGCCGNTATNNTCCCAACTTTTAATCTNAAATATGCATGATTANAANTATCAAAATATGATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8401141_8401190_ler-0_po-0_3484_26577_TRUE
SEQUENCE_TARGET=950,149
SEQUENCE_TEMPLATE=NCNTNTGAAGCTGTCAAATCCAATATAATCTCTATATTATAGCTTTNTCTNTATGATNTTGGAATTCTGTTTATACATAGTTTGTTTCTATATTATAGCTTTTTCGATATGATATTTTTCTTCTTCTTGCANTNTTNNNTTATAGNTGTTTNNATATGATTTTGGAATTATATTTAGCGAATGAATANGAAACANANTTTTTTTTGNGNAAAATGCAAAAAATCTTAANACAANGCAGCATATTGANTTTATGTGAATGNTAAANTATTTNNATATTATTTTCCTNNTGAAAGTGANGAGTNNNNNNNNNNNNNTCAAACGAAAATATCTAATGGNTAAACCANTAGTCTAGACNNTTTAAAGAATAATTNAANATNATTTATNTANAAAAANNNNNNNGNGAGACTGTGAGAAAGCCATNCCCATATAATATTTAGTAAGAAAATANTAATAAAATTACAGGNAGTAAAAANNTTTCCANACTTTATCAAAAACCNCTCAAACAGAAAATCCCAATTTAAGTATACAGAAAAGAGAAAACCCAATCCACACTTTCACTNATTTCNTCCCAATTTCTCANAANAATNANANAAANACCATCAAATCACAAATAATTNNNNNAATTCATTGCGGTGGTGCAACAAAGGCATGTTCCCAAGAGCGAAGATTTACTCCGTTTTCTGACTATGCCTTNCATNGAATCAGNNGANGCTTTCTTGTCCACCAAGNTATCTTNNGAGCCCTTGGTTTCTTTGAAGCTATCATTGTGTTTNTTCAANGACCCTAGAGANNNCTCGCTGCCTCTTGATTTCNCAGACTCACTTCTCTTCAGCATCACAGTTTTCTCTTCCACATCGATTGTTGCCTCGTATATCTCAGTCTTGTCCTCACAGAGTACCGGAGTATTCTCTACCGATTCATCGCGTCTTTCTTCGATTCTTAGGCCATGATCAAAGCTANGAGCTGGCATCTCAAATCTANCTTCTTTACTGCNTTCANTNATCGCTTCAATAGTCTCTTGCNTTTTGTTGCTTCCTACGCAAGGAACANGCAGAGCTGCAGACGTGTTAGCTTTCTCTAAACCCNTCTTGTTTCTTNNNGGAAACGCAACCTCTGAAACCAAATGTTGAACAAGCGAATCTCCAATAACTCTACTATTCTGGCTCACTGGGAGTGATCTACATCGATACATNCGTCTTAGACTCGCTTCTCTTTTCTCCTTCTCTTCTTTATAAACTCCAAGACCAGTGTCTTGATTTGCATTTGACTCTTGCGAACAAGGTTTAGAGTTGGAGATCGCCTTATCGGCTTTTCCTTTGTCTGTAACTTCTTCATCATGAACAATNCGATAGTCCAGATTCAGAATCTCCTGTGGCTCGATTTCAGGAAACTCTTCCCTTCCTNTTCTCTGTTCAATTCCAGTCTCTTGATCAAGCTTTACNACTTCCTCTGAATGAACAATTTCGTCCCGAGACTCATTCACCATNTTTTCATCAGTATCAACAGTTGCNAAACATCTTGGAGATTCAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAATCTCATCAATCANACAGAGCCTTTCTTCATTACCAGACACTGTCGAATTCAATTTGCTCACAACAGTTTGAGTTCCATTCATAGACAACACTTCCACTTNTCTTGCTTCACTCAAATTTTCAACACCTGATAATGAACANAGAGACAGGATTGCATTAGGTTTANTTTCAAATATGAAGAGAGGAGAGAAAAATTAAGATCAACCTTTCGCAACTGATGTCTGTTTNTGAAGCTTCTCAGATNTTTCCATTACCAAGCTCTCCAAGCCACTATGGTCCATCTCTTCTTCGAAATGTTTTACTTTCGGTTCATCAACTANATCCTTGCTATGTGGTTCTGTTTCTTCAACACCATCACACAAACTCTCTTTCTCTTCATCTCCATCGTTTCCTTCACCTGCAAGAAAGAACTGATCTTCCGTGGTCTCTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.168722
PRIMER_LEFT_0_PENALTY=0.095771
PRIMER_RIGHT_0_PENALTY=0.072951
PRIMER_LEFT_0_SEQUENCE=TCGCGTCTTTCTTCGATTCT
PRIMER_RIGHT_0_SEQUENCE=GGACCATAGTGGCTTGGAGA
PRIMER_LEFT_0=920,20
PRIMER_RIGHT_0=1897,20
PRIMER_LEFT_0_TM=60.096
PRIMER_RIGHT_0_TM=60.073
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=978
PRIMER_PAIR_1_PENALTY=0.176548
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_1_SEQUENCE=ACAGACAAAGGAAAAGCCGA
PRIMER_LEFT_1=639,20
PRIMER_RIGHT_1=1323,20
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=685
PRIMER_PAIR_2_PENALTY=0.215853
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.186600
PRIMER_LEFT_2_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_2_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_2=639,20
PRIMER_RIGHT_2=1391,20
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=753
PRIMER_PAIR_3_PENALTY=0.255521
PRIMER_LEFT_3_PENALTY=0.095771
PRIMER_RIGHT_3_PENALTY=0.159750
PRIMER_LEFT_3_SEQUENCE=TCGCGTCTTTCTTCGATTCT
PRIMER_RIGHT_3_SEQUENCE=CATCAGTTGCGAAAGGTTGA
PRIMER_LEFT_3=920,20
PRIMER_RIGHT_3=1838,20
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.840
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=919
PRIMER_PAIR_4_PENALTY=0.282371
PRIMER_LEFT_4_PENALTY=0.095771
PRIMER_RIGHT_4_PENALTY=0.186600
PRIMER_LEFT_4_SEQUENCE=TCGCGTCTTTCTTCGATTCT
PRIMER_RIGHT_4_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_4=920,20
PRIMER_RIGHT_4=1391,20
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=472
PRIMER_PAIR_5_PENALTY=0.308173
PRIMER_LEFT_5_PENALTY=0.235222
PRIMER_RIGHT_5_PENALTY=0.072951
PRIMER_LEFT_5_SEQUENCE=CTACCGATTCATCGCGTCTT
PRIMER_RIGHT_5_SEQUENCE=GGACCATAGTGGCTTGGAGA
PRIMER_LEFT_5=909,20
PRIMER_RIGHT_5=1897,20
PRIMER_LEFT_5_TM=60.235
PRIMER_RIGHT_5_TM=60.073
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=989
PRIMER_PAIR_6_PENALTY=0.312121
PRIMER_LEFT_6_PENALTY=0.029253
PRIMER_RIGHT_6_PENALTY=0.282868
PRIMER_LEFT_6_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_6_SEQUENCE=GTTTCAGAGGTTGCGTTTCC
PRIMER_LEFT_6=639,20
PRIMER_RIGHT_6=1119,20
PRIMER_LEFT_6_TM=59.971
PRIMER_RIGHT_6_TM=59.717
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=481
PRIMER_PAIR_7_PENALTY=0.312121
PRIMER_LEFT_7_PENALTY=0.029253
PRIMER_RIGHT_7_PENALTY=0.282868
PRIMER_LEFT_7_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_7_SEQUENCE=GGTTTCAGAGGTTGCGTTTC
PRIMER_LEFT_7=639,20
PRIMER_RIGHT_7=1120,20
PRIMER_LEFT_7_TM=59.971
PRIMER_RIGHT_7_TM=59.717
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=482
PRIMER_PAIR_8_PENALTY=0.352974
PRIMER_LEFT_8_PENALTY=0.029253
PRIMER_RIGHT_8_PENALTY=0.323721
PRIMER_LEFT_8_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_8_SEQUENCE=GTTTCCTGAAATCGAGCCAC
PRIMER_LEFT_8=639,20
PRIMER_RIGHT_8=1390,20
PRIMER_LEFT_8_TM=59.971
PRIMER_RIGHT_8_TM=59.676
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=752
PRIMER_PAIR_9_PENALTY=0.382517
PRIMER_LEFT_9_PENALTY=0.235222
PRIMER_RIGHT_9_PENALTY=0.147295
PRIMER_LEFT_9_SEQUENCE=CTACCGATTCATCGCGTCTT
PRIMER_RIGHT_9_SEQUENCE=ACAGACAAAGGAAAAGCCGA
PRIMER_LEFT_9=909,20
PRIMER_RIGHT_9=1323,20
PRIMER_LEFT_9_TM=60.235
PRIMER_RIGHT_9_TM=59.853
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=11.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=415
PRIMER_PAIR_10_PENALTY=0.394971
PRIMER_LEFT_10_PENALTY=0.235222
PRIMER_RIGHT_10_PENALTY=0.159750
PRIMER_LEFT_10_SEQUENCE=CTACCGATTCATCGCGTCTT
PRIMER_RIGHT_10_SEQUENCE=CATCAGTTGCGAAAGGTTGA
PRIMER_LEFT_10=909,20
PRIMER_RIGHT_10=1838,20
PRIMER_LEFT_10_TM=60.235
PRIMER_RIGHT_10_TM=59.840
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=930
PRIMER_PAIR_11_PENALTY=0.419491
PRIMER_LEFT_11_PENALTY=0.095771
PRIMER_RIGHT_11_PENALTY=0.323721
PRIMER_LEFT_11_SEQUENCE=TCGCGTCTTTCTTCGATTCT
PRIMER_RIGHT_11_SEQUENCE=GTTTCCTGAAATCGAGCCAC
PRIMER_LEFT_11=920,20
PRIMER_RIGHT_11=1390,20
PRIMER_LEFT_11_TM=60.096
PRIMER_RIGHT_11_TM=59.676
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=471
PRIMER_PAIR_12_PENALTY=0.421822
PRIMER_LEFT_12_PENALTY=0.235222
PRIMER_RIGHT_12_PENALTY=0.186600
PRIMER_LEFT_12_SEQUENCE=CTACCGATTCATCGCGTCTT
PRIMER_RIGHT_12_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_12=909,20
PRIMER_RIGHT_12=1391,20
PRIMER_LEFT_12_TM=60.235
PRIMER_RIGHT_12_TM=59.813
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=483
PRIMER_PAIR_13_PENALTY=0.425205
PRIMER_LEFT_13_PENALTY=0.352253
PRIMER_RIGHT_13_PENALTY=0.072951
PRIMER_LEFT_13_SEQUENCE=CATCGCGTCTTTCTTCGATT
PRIMER_RIGHT_13_SEQUENCE=GGACCATAGTGGCTTGGAGA
PRIMER_LEFT_13=918,20
PRIMER_RIGHT_13=1897,20
PRIMER_LEFT_13_TM=60.352
PRIMER_RIGHT_13_TM=60.073
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.6000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=980
PRIMER_PAIR_14_PENALTY=0.425205
PRIMER_LEFT_14_PENALTY=0.352253
PRIMER_RIGHT_14_PENALTY=0.072951
PRIMER_LEFT_14_SEQUENCE=CGATTCATCGCGTCTTTCTT
PRIMER_RIGHT_14_SEQUENCE=GGACCATAGTGGCTTGGAGA
PRIMER_LEFT_14=913,20
PRIMER_RIGHT_14=1897,20
PRIMER_LEFT_14_TM=60.352
PRIMER_RIGHT_14_TM=60.073
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=985
PRIMER_PAIR_15_PENALTY=0.481132
PRIMER_LEFT_15_PENALTY=0.408180
PRIMER_RIGHT_15_PENALTY=0.072951
PRIMER_LEFT_15_SEQUENCE=CGCGTCTTTCTTCGATTCTT
PRIMER_RIGHT_15_SEQUENCE=GGACCATAGTGGCTTGGAGA
PRIMER_LEFT_15=921,20
PRIMER_RIGHT_15=1897,20
PRIMER_LEFT_15_TM=59.592
PRIMER_RIGHT_15_TM=60.073
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=977
PRIMER_PAIR_16_PENALTY=0.512003
PRIMER_LEFT_16_PENALTY=0.352253
PRIMER_RIGHT_16_PENALTY=0.159750
PRIMER_LEFT_16_SEQUENCE=CATCGCGTCTTTCTTCGATT
PRIMER_RIGHT_16_SEQUENCE=CATCAGTTGCGAAAGGTTGA
PRIMER_LEFT_16=918,20
PRIMER_RIGHT_16=1838,20
PRIMER_LEFT_16_TM=60.352
PRIMER_RIGHT_16_TM=59.840
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.6000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=921
PRIMER_PAIR_17_PENALTY=0.512003
PRIMER_LEFT_17_PENALTY=0.352253
PRIMER_RIGHT_17_PENALTY=0.159750
PRIMER_LEFT_17_SEQUENCE=CGATTCATCGCGTCTTTCTT
PRIMER_RIGHT_17_SEQUENCE=CATCAGTTGCGAAAGGTTGA
PRIMER_LEFT_17=913,20
PRIMER_RIGHT_17=1838,20
PRIMER_LEFT_17_TM=60.352
PRIMER_RIGHT_17_TM=59.840
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=926
PRIMER_PAIR_18_PENALTY=0.538854
PRIMER_LEFT_18_PENALTY=0.352253
PRIMER_RIGHT_18_PENALTY=0.186600
PRIMER_LEFT_18_SEQUENCE=CGATTCATCGCGTCTTTCTT
PRIMER_RIGHT_18_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_18=913,20
PRIMER_RIGHT_18=1391,20
PRIMER_LEFT_18_TM=60.352
PRIMER_RIGHT_18_TM=59.813
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=479
PRIMER_PAIR_19_PENALTY=0.552166
PRIMER_LEFT_19_PENALTY=0.029253
PRIMER_RIGHT_19_PENALTY=0.522913
PRIMER_LEFT_19_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_19_SEQUENCE=TCGCAAGAGTCAAATGCAAA
PRIMER_LEFT_19=639,20
PRIMER_RIGHT_19=1275,20
PRIMER_LEFT_19_TM=59.971
PRIMER_RIGHT_19_TM=60.523
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=8.8000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=637
PRIMER_PAIR_20_PENALTY=0.555476
PRIMER_LEFT_20_PENALTY=0.408180
PRIMER_RIGHT_20_PENALTY=0.147295
PRIMER_LEFT_20_SEQUENCE=CGCGTCTTTCTTCGATTCTT
PRIMER_RIGHT_20_SEQUENCE=ACAGACAAAGGAAAAGCCGA
PRIMER_LEFT_20=921,20
PRIMER_RIGHT_20=1323,20
PRIMER_LEFT_20_TM=59.592
PRIMER_RIGHT_20_TM=59.853
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=403
PRIMER_PAIR_21_PENALTY=0.558942
PRIMER_LEFT_21_PENALTY=0.235222
PRIMER_RIGHT_21_PENALTY=0.323721
PRIMER_LEFT_21_SEQUENCE=CTACCGATTCATCGCGTCTT
PRIMER_RIGHT_21_SEQUENCE=GTTTCCTGAAATCGAGCCAC
PRIMER_LEFT_21=909,20
PRIMER_RIGHT_21=1390,20
PRIMER_LEFT_21_TM=60.235
PRIMER_RIGHT_21_TM=59.676
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=482
PRIMER_PAIR_22_PENALTY=0.567930
PRIMER_LEFT_22_PENALTY=0.408180
PRIMER_RIGHT_22_PENALTY=0.159750
PRIMER_LEFT_22_SEQUENCE=CGCGTCTTTCTTCGATTCTT
PRIMER_RIGHT_22_SEQUENCE=CATCAGTTGCGAAAGGTTGA
PRIMER_LEFT_22=921,20
PRIMER_RIGHT_22=1838,20
PRIMER_LEFT_22_TM=59.592
PRIMER_RIGHT_22_TM=59.840
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=918
PRIMER_PAIR_23_PENALTY=0.594781
PRIMER_LEFT_23_PENALTY=0.408180
PRIMER_RIGHT_23_PENALTY=0.186600
PRIMER_LEFT_23_SEQUENCE=CGCGTCTTTCTTCGATTCTT
PRIMER_RIGHT_23_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_23=921,20
PRIMER_RIGHT_23=1391,20
PRIMER_LEFT_23_TM=59.592
PRIMER_RIGHT_23_TM=59.813
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=471
PRIMER_PAIR_24_PENALTY=0.624632
PRIMER_LEFT_24_PENALTY=0.095771
PRIMER_RIGHT_24_PENALTY=0.528861
PRIMER_LEFT_24_SEQUENCE=TCGCGTCTTTCTTCGATTCT
PRIMER_RIGHT_24_SEQUENCE=TAGTGGCTTGGAGAGCTTGG
PRIMER_LEFT_24=920,20
PRIMER_RIGHT_24=1891,20
PRIMER_LEFT_24_TM=60.096
PRIMER_RIGHT_24_TM=60.529
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=972
PRIMER_PAIR_25_PENALTY=0.642468
PRIMER_LEFT_25_PENALTY=0.495173
PRIMER_RIGHT_25_PENALTY=0.147295
PRIMER_LEFT_25_SEQUENCE=ACAAAGGCATGTTCCCAAGA
PRIMER_RIGHT_25_SEQUENCE=ACAGACAAAGGAAAAGCCGA
PRIMER_LEFT_25=640,20
PRIMER_RIGHT_25=1323,20
PRIMER_LEFT_25_TM=60.495
PRIMER_RIGHT_25_TM=59.853
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=11.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=684
PRIMER_PAIR_26_PENALTY=0.655756
PRIMER_LEFT_26_PENALTY=0.095771
PRIMER_RIGHT_26_PENALTY=0.559985
PRIMER_LEFT_26_SEQUENCE=TCGCGTCTTTCTTCGATTCT
PRIMER_RIGHT_26_SEQUENCE=CAGACATCAGTTGCGAAAGG
PRIMER_LEFT_26=920,20
PRIMER_RIGHT_26=1842,20
PRIMER_LEFT_26_TM=60.096
PRIMER_RIGHT_26_TM=59.440
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=8.5000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=923
PRIMER_PAIR_27_PENALTY=0.675974
PRIMER_LEFT_27_PENALTY=0.352253
PRIMER_RIGHT_27_PENALTY=0.323721
PRIMER_LEFT_27_SEQUENCE=CATCGCGTCTTTCTTCGATT
PRIMER_RIGHT_27_SEQUENCE=GTTTCCTGAAATCGAGCCAC
PRIMER_LEFT_27=918,20
PRIMER_RIGHT_27=1390,20
PRIMER_LEFT_27_TM=60.352
PRIMER_RIGHT_27_TM=59.676
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.6000
PRIMER_RIGHT_27_END_STABILITY=9.4000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=473
PRIMER_PAIR_28_PENALTY=0.675974
PRIMER_LEFT_28_PENALTY=0.352253
PRIMER_RIGHT_28_PENALTY=0.323721
PRIMER_LEFT_28_SEQUENCE=CGATTCATCGCGTCTTTCTT
PRIMER_RIGHT_28_SEQUENCE=GTTTCCTGAAATCGAGCCAC
PRIMER_LEFT_28=913,20
PRIMER_RIGHT_28=1390,20
PRIMER_LEFT_28_TM=60.352
PRIMER_RIGHT_28_TM=59.676
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=478
PRIMER_PAIR_29_PENALTY=0.678790
PRIMER_LEFT_29_PENALTY=0.605838
PRIMER_RIGHT_29_PENALTY=0.072951
PRIMER_LEFT_29_SEQUENCE=TACCGATTCATCGCGTCTTT
PRIMER_RIGHT_29_SEQUENCE=GGACCATAGTGGCTTGGAGA
PRIMER_LEFT_29=910,20
PRIMER_RIGHT_29=1897,20
PRIMER_LEFT_29_TM=60.606
PRIMER_RIGHT_29_TM=60.073
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=988
PRIMER_PAIR_30_PENALTY=0.681773
PRIMER_LEFT_30_PENALTY=0.495173
PRIMER_RIGHT_30_PENALTY=0.186600
PRIMER_LEFT_30_SEQUENCE=ACAAAGGCATGTTCCCAAGA
PRIMER_RIGHT_30_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_30=640,20
PRIMER_RIGHT_30=1391,20
PRIMER_LEFT_30_TM=60.495
PRIMER_RIGHT_30_TM=59.813
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=752
PRIMER_PAIR_31_PENALTY=0.697568
PRIMER_LEFT_31_PENALTY=0.029253
PRIMER_RIGHT_31_PENALTY=0.668315
PRIMER_LEFT_31_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_31_SEQUENCE=TGGTTTCAGAGGTTGCGTTT
PRIMER_LEFT_31=639,20
PRIMER_RIGHT_31=1121,20
PRIMER_LEFT_31_TM=59.971
PRIMER_RIGHT_31_TM=60.668
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=8.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=483
PRIMER_PAIR_32_PENALTY=0.697568
PRIMER_LEFT_32_PENALTY=0.029253
PRIMER_RIGHT_32_PENALTY=0.668315
PRIMER_LEFT_32_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_32_SEQUENCE=TTGGTTTCAGAGGTTGCGTT
PRIMER_LEFT_32=639,20
PRIMER_RIGHT_32=1122,20
PRIMER_LEFT_32_TM=59.971
PRIMER_RIGHT_32_TM=60.668
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=484
PRIMER_PAIR_33_PENALTY=0.697568
PRIMER_LEFT_33_PENALTY=0.029253
PRIMER_RIGHT_33_PENALTY=0.668315
PRIMER_LEFT_33_SEQUENCE=AACAAAGGCATGTTCCCAAG
PRIMER_RIGHT_33_SEQUENCE=TTTGGTTTCAGAGGTTGCGT
PRIMER_LEFT_33=639,20
PRIMER_RIGHT_33=1123,20
PRIMER_LEFT_33_TM=59.971
PRIMER_RIGHT_33_TM=60.668
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=9.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=485
PRIMER_PAIR_34_PENALTY=0.731901
PRIMER_LEFT_34_PENALTY=0.408180
PRIMER_RIGHT_34_PENALTY=0.323721
PRIMER_LEFT_34_SEQUENCE=CGCGTCTTTCTTCGATTCTT
PRIMER_RIGHT_34_SEQUENCE=GTTTCCTGAAATCGAGCCAC
PRIMER_LEFT_34=921,20
PRIMER_RIGHT_34=1390,20
PRIMER_LEFT_34_TM=59.592
PRIMER_RIGHT_34_TM=59.676
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=470
PRIMER_PAIR_35_PENALTY=0.764082
PRIMER_LEFT_35_PENALTY=0.235222
PRIMER_RIGHT_35_PENALTY=0.528861
PRIMER_LEFT_35_SEQUENCE=CTACCGATTCATCGCGTCTT
PRIMER_RIGHT_35_SEQUENCE=TAGTGGCTTGGAGAGCTTGG
PRIMER_LEFT_35=909,20
PRIMER_RIGHT_35=1891,20
PRIMER_LEFT_35_TM=60.235
PRIMER_RIGHT_35_TM=60.529
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=983
PRIMER_PAIR_36_PENALTY=0.765588
PRIMER_LEFT_36_PENALTY=0.605838
PRIMER_RIGHT_36_PENALTY=0.159750
PRIMER_LEFT_36_SEQUENCE=TACCGATTCATCGCGTCTTT
PRIMER_RIGHT_36_SEQUENCE=CATCAGTTGCGAAAGGTTGA
PRIMER_LEFT_36=910,20
PRIMER_RIGHT_36=1838,20
PRIMER_LEFT_36_TM=60.606
PRIMER_RIGHT_36_TM=59.840
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=929
PRIMER_PAIR_37_PENALTY=0.773711
PRIMER_LEFT_37_PENALTY=0.626415
PRIMER_RIGHT_37_PENALTY=0.147295
PRIMER_LEFT_37_SEQUENCE=CAAAGGCATGTTCCCAAGAG
PRIMER_RIGHT_37_SEQUENCE=ACAGACAAAGGAAAAGCCGA
PRIMER_LEFT_37=641,20
PRIMER_RIGHT_37=1323,20
PRIMER_LEFT_37_TM=60.626
PRIMER_RIGHT_37_TM=59.853
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=11.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=683
PRIMER_PAIR_38_PENALTY=0.778040
PRIMER_LEFT_38_PENALTY=0.495173
PRIMER_RIGHT_38_PENALTY=0.282868
PRIMER_LEFT_38_SEQUENCE=ACAAAGGCATGTTCCCAAGA
PRIMER_RIGHT_38_SEQUENCE=GTTTCAGAGGTTGCGTTTCC
PRIMER_LEFT_38=640,20
PRIMER_RIGHT_38=1119,20
PRIMER_LEFT_38_TM=60.495
PRIMER_RIGHT_38_TM=59.717
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=480
PRIMER_PAIR_39_PENALTY=0.778040
PRIMER_LEFT_39_PENALTY=0.495173
PRIMER_RIGHT_39_PENALTY=0.282868
PRIMER_LEFT_39_SEQUENCE=ACAAAGGCATGTTCCCAAGA
PRIMER_RIGHT_39_SEQUENCE=GGTTTCAGAGGTTGCGTTTC
PRIMER_LEFT_39=640,20
PRIMER_RIGHT_39=1120,20
PRIMER_LEFT_39_TM=60.495
PRIMER_RIGHT_39_TM=59.717
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=481
PRIMER_PAIR_40_PENALTY=0.792439
PRIMER_LEFT_40_PENALTY=0.605838
PRIMER_RIGHT_40_PENALTY=0.186600
PRIMER_LEFT_40_SEQUENCE=TACCGATTCATCGCGTCTTT
PRIMER_RIGHT_40_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_40=910,20
PRIMER_RIGHT_40=1391,20
PRIMER_LEFT_40_TM=60.606
PRIMER_RIGHT_40_TM=59.813
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=482
PRIMER_PAIR_41_PENALTY=0.795206
PRIMER_LEFT_41_PENALTY=0.235222
PRIMER_RIGHT_41_PENALTY=0.559985
PRIMER_LEFT_41_SEQUENCE=CTACCGATTCATCGCGTCTT
PRIMER_RIGHT_41_SEQUENCE=CAGACATCAGTTGCGAAAGG
PRIMER_LEFT_41=909,20
PRIMER_RIGHT_41=1842,20
PRIMER_LEFT_41_TM=60.235
PRIMER_RIGHT_41_TM=59.440
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=934
PRIMER_PAIR_42_PENALTY=0.813016
PRIMER_LEFT_42_PENALTY=0.626415
PRIMER_RIGHT_42_PENALTY=0.186600
PRIMER_LEFT_42_SEQUENCE=CAAAGGCATGTTCCCAAGAG
PRIMER_RIGHT_42_SEQUENCE=AGTTTCCTGAAATCGAGCCA
PRIMER_LEFT_42=641,20
PRIMER_RIGHT_42=1391,20
PRIMER_LEFT_42_TM=60.626
PRIMER_RIGHT_42_TM=59.813
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=9.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=751
PRIMER_PAIR_43_PENALTY=0.818893
PRIMER_LEFT_43_PENALTY=0.495173
PRIMER_RIGHT_43_PENALTY=0.323721
PRIMER_LEFT_43_SEQUENCE=ACAAAGGCATGTTCCCAAGA
PRIMER_RIGHT_43_SEQUENCE=GTTTCCTGAAATCGAGCCAC
PRIMER_LEFT_43=640,20
PRIMER_RIGHT_43=1390,20
PRIMER_LEFT_43_TM=60.495
PRIMER_RIGHT_43_TM=59.676
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=751
PRIMER_PAIR_44_PENALTY=0.881114
PRIMER_LEFT_44_PENALTY=0.352253
PRIMER_RIGHT_44_PENALTY=0.528861
PRIMER_LEFT_44_SEQUENCE=CATCGCGTCTTTCTTCGATT
PRIMER_RIGHT_44_SEQUENCE=TAGTGGCTTGGAGAGCTTGG
PRIMER_LEFT_44=918,20
PRIMER_RIGHT_44=1891,20
PRIMER_LEFT_44_TM=60.352
PRIMER_RIGHT_44_TM=60.529
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.6000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=974
PRIMER_PAIR_45_PENALTY=0.881114
PRIMER_LEFT_45_PENALTY=0.352253
PRIMER_RIGHT_45_PENALTY=0.528861
PRIMER_LEFT_45_SEQUENCE=CGATTCATCGCGTCTTTCTT
PRIMER_RIGHT_45_SEQUENCE=TAGTGGCTTGGAGAGCTTGG
PRIMER_LEFT_45=913,20
PRIMER_RIGHT_45=1891,20
PRIMER_LEFT_45_TM=60.352
PRIMER_RIGHT_45_TM=60.529
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=979
PRIMER_PAIR_46_PENALTY=0.909283
PRIMER_LEFT_46_PENALTY=0.626415
PRIMER_RIGHT_46_PENALTY=0.282868
PRIMER_LEFT_46_SEQUENCE=CAAAGGCATGTTCCCAAGAG
PRIMER_RIGHT_46_SEQUENCE=GTTTCAGAGGTTGCGTTTCC
PRIMER_LEFT_46=641,20
PRIMER_RIGHT_46=1119,20
PRIMER_LEFT_46_TM=60.626
PRIMER_RIGHT_46_TM=59.717
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.5000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=479
PRIMER_PAIR_47_PENALTY=0.909283
PRIMER_LEFT_47_PENALTY=0.626415
PRIMER_RIGHT_47_PENALTY=0.282868
PRIMER_LEFT_47_SEQUENCE=CAAAGGCATGTTCCCAAGAG
PRIMER_RIGHT_47_SEQUENCE=GGTTTCAGAGGTTGCGTTTC
PRIMER_LEFT_47=641,20
PRIMER_RIGHT_47=1120,20
PRIMER_LEFT_47_TM=60.626
PRIMER_RIGHT_47_TM=59.717
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=480
PRIMER_PAIR_48_PENALTY=0.912238
PRIMER_LEFT_48_PENALTY=0.352253
PRIMER_RIGHT_48_PENALTY=0.559985
PRIMER_LEFT_48_SEQUENCE=CATCGCGTCTTTCTTCGATT
PRIMER_RIGHT_48_SEQUENCE=CAGACATCAGTTGCGAAAGG
PRIMER_LEFT_48=918,20
PRIMER_RIGHT_48=1842,20
PRIMER_LEFT_48_TM=60.352
PRIMER_RIGHT_48_TM=59.440
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.6000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=925
PRIMER_PAIR_49_PENALTY=0.912238
PRIMER_LEFT_49_PENALTY=0.352253
PRIMER_RIGHT_49_PENALTY=0.559985
PRIMER_LEFT_49_SEQUENCE=CGATTCATCGCGTCTTTCTT
PRIMER_RIGHT_49_SEQUENCE=CAGACATCAGTTGCGAAAGG
PRIMER_LEFT_49=913,20
PRIMER_RIGHT_49=1842,20
PRIMER_LEFT_49_TM=60.352
PRIMER_RIGHT_49_TM=59.440
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=930
=
SEQUENCE_ID=MAGIC.426_4_10907896_10908075_edi-0_bur-0_949100_304_TRUE
SEQUENCE_TARGET=950,279
SEQUENCE_TEMPLATE=ACNGCNGTGTCTCTAATGGAAGCNGTTCTTGCCATCGATTGAGAGGAAGTCATCGTGGTNGTTCTGGTGAANGANGAATCNCCTTTTCGAGATGATCTTACNGGCTTTCCTTTGCTGAAACCNGGTAGGTATAGACACAAAGCGCTGCATTTGAATCCGTCTTCGAATGAATTGATGCTTTTNGAATTCGCNGTATTGCTATTGCTTCTGTTGCTTTTGTTAGACTCTGTTTTGGAGAAGAAAGANTTGTTTCCACTACTCTTGTGAGAGAGACTCTTAGAGTTAGAGGAAGAGAAGCCACGATGATGATTTTGATTATGATGNGCATTTCTTGATTTATGCAATAGCATGTCGAAATCGAGCGATGGTGTGCAAGCACGTCCTTCGCCGCAAGATTTGCTTCTTCGAAACGCAGTGGCNGCATCTTGAACGAGCATTAGGTCAAGAACTTGCTCAGTNGTTCTGTCCTTCCAGTTGTGAATAGAGCTATTTCTAGGTGACGTNGCTGCTGAGTTTGGGAGGCTGCAGCTCACAAACTTCGTCTTCATTTGATGATCNNNNNNNNNGTCATCGATGTCTCTCGGACGTGAGACTATCATATCGAAGCTCCCATTCCTNGACAATAGCGATTGCGGATCAACCGAGATCCGCCTCTCGGATACGGATTGCATACTCGTCTTCTTGATAAGAATCTTGGGCTCGTCTCCACCACCTAGAACCTCAAAGTCATGCCAGTCCTCGAGACTACTATCGTCCCTGAAGCTGAGAACATCTGTNNCCATTTGTTNCTCTTTGGCCTGNAANNGAATGAGGACTAATAAGAAAATGAAAATATTGGCAAAANGNCTAANACAGATTGATTGATACTTAAATACGGAGCAGGATGGGTTGCACGTCGGTGNCTACNTTAGTCATGACTTGCACGTTTAATATAATATAACCCAAAGTGTTGTTGACTTCATCTNCTAAAAAANGAAAAATGNNTCATNNTTTTTTTATTNGTTAANTATCNCANTATCGTTGACATGAGACCATNCCCANGATCAAGACAAGATCAAAAAGATAGATCTGCCTGCACGAACTACCTAATTATAATATTACCAAAGNATTTGGAGGGTNGTAATCTGCATCAGTGAAGACCATAAGTAGAAGCCATAAATCTTGAATACNTCTNCNNNTNNATGNACNTNGTNCTNTATNNNNTACNTNNTACTTGGNNANTTNTTTNTNNTTGGTNACNTNTNNTTNTCNTTGNTNACTNTNCNCGNNANACTTNGNTATANNNCTTNNTNATANNNCTTNTNNNTNNNTCTNNTATTTTTNNNTTNNNNTNNNTTTTNCNNNNNNNNNNNNNNNNNCTTTGAATTTTNNTGTGTCTTTTNCTACAAATATTTCTTCACTTTCATTGAAATTCATCNCCTTCAATGGATGCACNTACGTCCGTTAGTCATNNNTGGCTTTAGGCCTCATATNTATTCTAGNTGCTCTAGTATATCCCTCNAAAATGGACGGTGAGAAACAATACCAATTTTTNATCCCAAGGGATTTGAACCGGTTATGTTTGAATAACATAGNGATTTGAACGTCAAATCAACAANTTACGAAACGATTAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATGCTAAAACATTTTTGAANGTTTTCAAATATGGATTTGATCGACAAANATAGAAGTCATATACTCCTTGATTTTTCCTTGGGGATTGGTGNATCCTTCATTTNCATTGTAGGNGACGNTCATCATNCGTGNAAGATCAATGGTTGCCTTAAGAAGAAAATGTGGCACGCCAGTTGTTGNCAAGAACTCCTCATTTAGTGTCTTATTGGCANCTNCAACNATTTTANNAAGCTCCCTAAAAGNTTCCTCTTTGGTAACCCCATATTGCTTCATATANCAGTTNACCGCGTTCGNGANATATCCTCTACTCATGTCNTCCTACTCAATATTTGNGTAAGTNGGTAAGATTTACAAGAAAGCAGTTATACGCGAGATGTAAAGAAANNATTTATGTATTTGTACCTCATAACCCATNAAATCATTNATA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.350503
PRIMER_LEFT_0_PENALTY=0.083883
PRIMER_RIGHT_0_PENALTY=0.266620
PRIMER_LEFT_0_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_0_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_0=868,20
PRIMER_RIGHT_0=1839,20
PRIMER_LEFT_0_TM=59.916
PRIMER_RIGHT_0_TM=59.733
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=972
PRIMER_PAIR_1_PENALTY=0.566115
PRIMER_LEFT_1_PENALTY=0.083883
PRIMER_RIGHT_1_PENALTY=0.482233
PRIMER_LEFT_1_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_1_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_1=868,20
PRIMER_RIGHT_1=1840,20
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.482
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=0.979374
PRIMER_LEFT_2_PENALTY=0.712754
PRIMER_RIGHT_2_PENALTY=0.266620
PRIMER_LEFT_2_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_2_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_2=870,20
PRIMER_RIGHT_2=1839,20
PRIMER_LEFT_2_TM=60.713
PRIMER_RIGHT_2_TM=59.733
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=970
PRIMER_PAIR_3_PENALTY=0.979374
PRIMER_LEFT_3_PENALTY=0.712754
PRIMER_RIGHT_3_PENALTY=0.266620
PRIMER_LEFT_3_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_3_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_3=869,20
PRIMER_RIGHT_3=1839,20
PRIMER_LEFT_3_TM=60.713
PRIMER_RIGHT_3_TM=59.733
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=971
PRIMER_PAIR_4_PENALTY=1.194986
PRIMER_LEFT_4_PENALTY=0.712754
PRIMER_RIGHT_4_PENALTY=0.482233
PRIMER_LEFT_4_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_4_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_4=870,20
PRIMER_RIGHT_4=1840,20
PRIMER_LEFT_4_TM=60.713
PRIMER_RIGHT_4_TM=60.482
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=971
PRIMER_PAIR_5_PENALTY=1.194986
PRIMER_LEFT_5_PENALTY=0.712754
PRIMER_RIGHT_5_PENALTY=0.482233
PRIMER_LEFT_5_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_5_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_5=869,20
PRIMER_RIGHT_5=1840,20
PRIMER_LEFT_5_TM=60.713
PRIMER_RIGHT_5_TM=60.482
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=972
PRIMER_PAIR_6_PENALTY=1.227016
PRIMER_LEFT_6_PENALTY=0.083883
PRIMER_RIGHT_6_PENALTY=1.143133
PRIMER_LEFT_6_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_6_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_6=868,20
PRIMER_RIGHT_6=1835,20
PRIMER_LEFT_6_TM=59.916
PRIMER_RIGHT_6_TM=61.143
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=968
PRIMER_PAIR_7_PENALTY=1.227016
PRIMER_LEFT_7_PENALTY=0.083883
PRIMER_RIGHT_7_PENALTY=1.143133
PRIMER_LEFT_7_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_7_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_7=868,20
PRIMER_RIGHT_7=1836,20
PRIMER_LEFT_7_TM=59.916
PRIMER_RIGHT_7_TM=61.143
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=969
PRIMER_PAIR_8_PENALTY=1.239333
PRIMER_LEFT_8_PENALTY=0.083883
PRIMER_RIGHT_8_PENALTY=1.155450
PRIMER_LEFT_8_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_8_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_8=868,20
PRIMER_RIGHT_8=1525,21
PRIMER_LEFT_8_TM=59.916
PRIMER_RIGHT_8_TM=59.845
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=658
PRIMER_PAIR_9_PENALTY=1.239333
PRIMER_LEFT_9_PENALTY=0.083883
PRIMER_RIGHT_9_PENALTY=1.155450
PRIMER_LEFT_9_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_9_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_9=868,20
PRIMER_RIGHT_9=1526,21
PRIMER_LEFT_9_TM=59.916
PRIMER_RIGHT_9_TM=59.845
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=659
PRIMER_PAIR_10_PENALTY=1.465064
PRIMER_LEFT_10_PENALTY=0.083883
PRIMER_RIGHT_10_PENALTY=1.381181
PRIMER_LEFT_10_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_10_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_10=868,20
PRIMER_RIGHT_10=1834,20
PRIMER_LEFT_10_TM=59.916
PRIMER_RIGHT_10_TM=58.619
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=967
PRIMER_PAIR_11_PENALTY=1.581829
PRIMER_LEFT_11_PENALTY=0.426379
PRIMER_RIGHT_11_PENALTY=1.155450
PRIMER_LEFT_11_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_11_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_11=700,20
PRIMER_RIGHT_11=1525,21
PRIMER_LEFT_11_TM=59.574
PRIMER_RIGHT_11_TM=59.845
PRIMER_LEFT_11_GC_PERCENT=60.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=826
PRIMER_PAIR_12_PENALTY=1.581829
PRIMER_LEFT_12_PENALTY=0.426379
PRIMER_RIGHT_12_PENALTY=1.155450
PRIMER_LEFT_12_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_12_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_12=700,20
PRIMER_RIGHT_12=1526,21
PRIMER_LEFT_12_TM=59.574
PRIMER_RIGHT_12_TM=59.845
PRIMER_LEFT_12_GC_PERCENT=60.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=827
PRIMER_PAIR_13_PENALTY=1.637322
PRIMER_LEFT_13_PENALTY=0.083883
PRIMER_RIGHT_13_PENALTY=1.553439
PRIMER_LEFT_13_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_13_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_13=868,20
PRIMER_RIGHT_13=1834,21
PRIMER_LEFT_13_TM=59.916
PRIMER_RIGHT_13_TM=59.447
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=967
PRIMER_PAIR_14_PENALTY=1.669226
PRIMER_LEFT_14_PENALTY=0.513776
PRIMER_RIGHT_14_PENALTY=1.155450
PRIMER_LEFT_14_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_14_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_14=596,20
PRIMER_RIGHT_14=1525,21
PRIMER_LEFT_14_TM=59.486
PRIMER_RIGHT_14_TM=59.845
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=930
PRIMER_PAIR_15_PENALTY=1.669226
PRIMER_LEFT_15_PENALTY=0.513776
PRIMER_RIGHT_15_PENALTY=1.155450
PRIMER_LEFT_15_SEQUENCE=CATATCGAAGCTCCCATTCC
PRIMER_RIGHT_15_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_15=596,20
PRIMER_RIGHT_15=1526,21
PRIMER_LEFT_15_TM=59.486
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=931
PRIMER_PAIR_16_PENALTY=1.811843
PRIMER_LEFT_16_PENALTY=0.656393
PRIMER_RIGHT_16_PENALTY=1.155450
PRIMER_LEFT_16_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_16_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_16=718,20
PRIMER_RIGHT_16=1525,21
PRIMER_LEFT_16_TM=60.656
PRIMER_RIGHT_16_TM=59.845
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=808
PRIMER_PAIR_17_PENALTY=1.811843
PRIMER_LEFT_17_PENALTY=0.656393
PRIMER_RIGHT_17_PENALTY=1.155450
PRIMER_LEFT_17_SEQUENCE=CCTCAAAGTCATGCCAGTCC
PRIMER_RIGHT_17_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_17=718,20
PRIMER_RIGHT_17=1526,21
PRIMER_LEFT_17_TM=60.656
PRIMER_RIGHT_17_TM=59.845
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=809
PRIMER_PAIR_18_PENALTY=1.855887
PRIMER_LEFT_18_PENALTY=0.712754
PRIMER_RIGHT_18_PENALTY=1.143133
PRIMER_LEFT_18_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_18_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_18=870,20
PRIMER_RIGHT_18=1835,20
PRIMER_LEFT_18_TM=60.713
PRIMER_RIGHT_18_TM=61.143
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=966
PRIMER_PAIR_19_PENALTY=1.855887
PRIMER_LEFT_19_PENALTY=0.712754
PRIMER_RIGHT_19_PENALTY=1.143133
PRIMER_LEFT_19_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_19_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_19=870,20
PRIMER_RIGHT_19=1836,20
PRIMER_LEFT_19_TM=60.713
PRIMER_RIGHT_19_TM=61.143
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=967
PRIMER_PAIR_20_PENALTY=1.855887
PRIMER_LEFT_20_PENALTY=0.712754
PRIMER_RIGHT_20_PENALTY=1.143133
PRIMER_LEFT_20_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_20_SEQUENCE=CCCCAAGGAAAAATCAAGGA
PRIMER_LEFT_20=869,20
PRIMER_RIGHT_20=1835,20
PRIMER_LEFT_20_TM=60.713
PRIMER_RIGHT_20_TM=61.143
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=967
PRIMER_PAIR_21_PENALTY=1.855887
PRIMER_LEFT_21_PENALTY=0.712754
PRIMER_RIGHT_21_PENALTY=1.143133
PRIMER_LEFT_21_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_21_SEQUENCE=TCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_21=869,20
PRIMER_RIGHT_21=1836,20
PRIMER_LEFT_21_TM=60.713
PRIMER_RIGHT_21_TM=61.143
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=968
PRIMER_PAIR_22_PENALTY=1.868204
PRIMER_LEFT_22_PENALTY=0.712754
PRIMER_RIGHT_22_PENALTY=1.155450
PRIMER_LEFT_22_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_22_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_22=870,20
PRIMER_RIGHT_22=1525,21
PRIMER_LEFT_22_TM=60.713
PRIMER_RIGHT_22_TM=59.845
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=656
PRIMER_PAIR_23_PENALTY=1.868204
PRIMER_LEFT_23_PENALTY=0.712754
PRIMER_RIGHT_23_PENALTY=1.155450
PRIMER_LEFT_23_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_23_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_23=870,20
PRIMER_RIGHT_23=1526,21
PRIMER_LEFT_23_TM=60.713
PRIMER_RIGHT_23_TM=59.845
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=657
PRIMER_PAIR_24_PENALTY=1.868204
PRIMER_LEFT_24_PENALTY=0.712754
PRIMER_RIGHT_24_PENALTY=1.155450
PRIMER_LEFT_24_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_24_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_24=869,20
PRIMER_RIGHT_24=1525,21
PRIMER_LEFT_24_TM=60.713
PRIMER_RIGHT_24_TM=59.845
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=657
PRIMER_PAIR_25_PENALTY=1.868204
PRIMER_LEFT_25_PENALTY=0.712754
PRIMER_RIGHT_25_PENALTY=1.155450
PRIMER_LEFT_25_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_25_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_25=869,20
PRIMER_RIGHT_25=1526,21
PRIMER_LEFT_25_TM=60.713
PRIMER_RIGHT_25_TM=59.845
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=658
PRIMER_PAIR_26_PENALTY=1.890385
PRIMER_LEFT_26_PENALTY=0.734934
PRIMER_RIGHT_26_PENALTY=1.155450
PRIMER_LEFT_26_SEQUENCE=AGAATCTTGGGCTCGTCTCC
PRIMER_RIGHT_26_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_26=687,20
PRIMER_RIGHT_26=1525,21
PRIMER_LEFT_26_TM=60.735
PRIMER_RIGHT_26_TM=59.845
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=839
PRIMER_PAIR_27_PENALTY=1.890385
PRIMER_LEFT_27_PENALTY=0.734934
PRIMER_RIGHT_27_PENALTY=1.155450
PRIMER_LEFT_27_SEQUENCE=AGAATCTTGGGCTCGTCTCC
PRIMER_RIGHT_27_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_27=687,20
PRIMER_RIGHT_27=1526,21
PRIMER_LEFT_27_TM=60.735
PRIMER_RIGHT_27_TM=59.845
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=840
PRIMER_PAIR_28_PENALTY=1.963785
PRIMER_LEFT_28_PENALTY=1.697165
PRIMER_RIGHT_28_PENALTY=0.266620
PRIMER_LEFT_28_SEQUENCE=TAAATACGGAGCAGGATGGGT
PRIMER_RIGHT_28_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_28=868,21
PRIMER_RIGHT_28=1839,20
PRIMER_LEFT_28_TM=60.697
PRIMER_RIGHT_28_TM=59.733
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=972
PRIMER_PAIR_29_PENALTY=2.001683
PRIMER_LEFT_29_PENALTY=0.049863
PRIMER_RIGHT_29_PENALTY=1.951820
PRIMER_LEFT_29_SEQUENCE=ATCGTCCCTGAAGCTGAGAA
PRIMER_RIGHT_29_SEQUENCE=TGTTTCTCACCGTCCATTTT
PRIMER_LEFT_29=749,20
PRIMER_RIGHT_29=1520,20
PRIMER_LEFT_29_TM=59.950
PRIMER_RIGHT_29_TM=58.048
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.2000
PRIMER_PAIR_29_COMPL_ANY=7.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=772
PRIMER_PAIR_30_PENALTY=2.001683
PRIMER_LEFT_30_PENALTY=0.049863
PRIMER_RIGHT_30_PENALTY=1.951820
PRIMER_LEFT_30_SEQUENCE=ATCGTCCCTGAAGCTGAGAA
PRIMER_RIGHT_30_SEQUENCE=TTGTTTCTCACCGTCCATTT
PRIMER_LEFT_30=749,20
PRIMER_RIGHT_30=1521,20
PRIMER_LEFT_30_TM=59.950
PRIMER_RIGHT_30_TM=58.048
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=7.2000
PRIMER_PAIR_30_COMPL_ANY=7.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=773
PRIMER_PAIR_31_PENALTY=2.022196
PRIMER_LEFT_31_PENALTY=0.083883
PRIMER_RIGHT_31_PENALTY=1.938313
PRIMER_LEFT_31_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_31_SEQUENCE=CACCAATCCCCAAGGAAAAA
PRIMER_LEFT_31=868,20
PRIMER_RIGHT_31=1842,20
PRIMER_LEFT_31_TM=59.916
PRIMER_RIGHT_31_TM=61.938
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=975
PRIMER_PAIR_32_PENALTY=2.035703
PRIMER_LEFT_32_PENALTY=0.083883
PRIMER_RIGHT_32_PENALTY=1.951820
PRIMER_LEFT_32_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_32_SEQUENCE=TGTTTCTCACCGTCCATTTT
PRIMER_LEFT_32=868,20
PRIMER_RIGHT_32=1520,20
PRIMER_LEFT_32_TM=59.916
PRIMER_RIGHT_32_TM=58.048
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.6000
PRIMER_RIGHT_32_END_STABILITY=7.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=653
PRIMER_PAIR_33_PENALTY=2.035703
PRIMER_LEFT_33_PENALTY=0.083883
PRIMER_RIGHT_33_PENALTY=1.951820
PRIMER_LEFT_33_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_33_SEQUENCE=TTGTTTCTCACCGTCCATTT
PRIMER_LEFT_33=868,20
PRIMER_RIGHT_33=1521,20
PRIMER_LEFT_33_TM=59.916
PRIMER_RIGHT_33_TM=58.048
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.6000
PRIMER_RIGHT_33_END_STABILITY=7.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=654
PRIMER_PAIR_34_PENALTY=2.093935
PRIMER_LEFT_34_PENALTY=0.712754
PRIMER_RIGHT_34_PENALTY=1.381181
PRIMER_LEFT_34_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_34_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_34=870,20
PRIMER_RIGHT_34=1834,20
PRIMER_LEFT_34_TM=60.713
PRIMER_RIGHT_34_TM=58.619
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=965
PRIMER_PAIR_35_PENALTY=2.093935
PRIMER_LEFT_35_PENALTY=0.712754
PRIMER_RIGHT_35_PENALTY=1.381181
PRIMER_LEFT_35_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_35_SEQUENCE=CCCAAGGAAAAATCAAGGAG
PRIMER_LEFT_35=869,20
PRIMER_RIGHT_35=1834,20
PRIMER_LEFT_35_TM=60.713
PRIMER_RIGHT_35_TM=58.619
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=966
PRIMER_PAIR_36_PENALTY=2.117153
PRIMER_LEFT_36_PENALTY=0.083883
PRIMER_RIGHT_36_PENALTY=2.033270
PRIMER_LEFT_36_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_36_SEQUENCE=ATCCCCAAGGAAAAATCAAG
PRIMER_LEFT_36=868,20
PRIMER_RIGHT_36=1837,20
PRIMER_LEFT_36_TM=59.916
PRIMER_RIGHT_36_TM=57.967
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.6000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=970
PRIMER_PAIR_37_PENALTY=2.179398
PRIMER_LEFT_37_PENALTY=1.697165
PRIMER_RIGHT_37_PENALTY=0.482233
PRIMER_LEFT_37_SEQUENCE=TAAATACGGAGCAGGATGGGT
PRIMER_RIGHT_37_SEQUENCE=CCAATCCCCAAGGAAAAATC
PRIMER_LEFT_37=868,21
PRIMER_RIGHT_37=1840,20
PRIMER_LEFT_37_TM=60.697
PRIMER_RIGHT_37_TM=60.482
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=973
PRIMER_PAIR_38_PENALTY=2.266193
PRIMER_LEFT_38_PENALTY=0.712754
PRIMER_RIGHT_38_PENALTY=1.553439
PRIMER_LEFT_38_SEQUENCE=AATACGGAGCAGGATGGGTT
PRIMER_RIGHT_38_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_38=870,20
PRIMER_RIGHT_38=1834,21
PRIMER_LEFT_38_TM=60.713
PRIMER_RIGHT_38_TM=59.447
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=965
PRIMER_PAIR_39_PENALTY=2.266193
PRIMER_LEFT_39_PENALTY=0.712754
PRIMER_RIGHT_39_PENALTY=1.553439
PRIMER_LEFT_39_SEQUENCE=AAATACGGAGCAGGATGGGT
PRIMER_RIGHT_39_SEQUENCE=CCCAAGGAAAAATCAAGGAGT
PRIMER_LEFT_39=869,20
PRIMER_RIGHT_39=1834,21
PRIMER_LEFT_39_TM=60.713
PRIMER_RIGHT_39_TM=59.447
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=966
PRIMER_PAIR_40_PENALTY=2.296405
PRIMER_LEFT_40_PENALTY=1.140954
PRIMER_RIGHT_40_PENALTY=1.155450
PRIMER_LEFT_40_SEQUENCE=CTCAAAGTCATGCCAGTCCTC
PRIMER_RIGHT_40_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_40=719,21
PRIMER_RIGHT_40=1525,21
PRIMER_LEFT_40_TM=59.859
PRIMER_RIGHT_40_TM=59.845
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=807
PRIMER_PAIR_41_PENALTY=2.296405
PRIMER_LEFT_41_PENALTY=1.140954
PRIMER_RIGHT_41_PENALTY=1.155450
PRIMER_LEFT_41_SEQUENCE=CTCAAAGTCATGCCAGTCCTC
PRIMER_RIGHT_41_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_41=719,21
PRIMER_RIGHT_41=1526,21
PRIMER_LEFT_41_TM=59.859
PRIMER_RIGHT_41_TM=59.845
PRIMER_LEFT_41_GC_PERCENT=52.381
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=808
PRIMER_PAIR_42_PENALTY=2.310684
PRIMER_LEFT_42_PENALTY=0.083883
PRIMER_RIGHT_42_PENALTY=2.226801
PRIMER_LEFT_42_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_42_SEQUENCE=ACCAATCCCCAAGGAAAAATC
PRIMER_LEFT_42=868,20
PRIMER_RIGHT_42=1841,21
PRIMER_LEFT_42_TM=59.916
PRIMER_RIGHT_42_TM=61.227
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.6000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=974
PRIMER_PAIR_43_PENALTY=2.353296
PRIMER_LEFT_43_PENALTY=1.197845
PRIMER_RIGHT_43_PENALTY=1.155450
PRIMER_LEFT_43_SEQUENCE=TCAAAGTCATGCCAGTCCTC
PRIMER_RIGHT_43_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_43=720,20
PRIMER_RIGHT_43=1525,21
PRIMER_LEFT_43_TM=58.802
PRIMER_RIGHT_43_TM=59.845
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=806
PRIMER_PAIR_44_PENALTY=2.353296
PRIMER_LEFT_44_PENALTY=1.197845
PRIMER_RIGHT_44_PENALTY=1.155450
PRIMER_LEFT_44_SEQUENCE=TCAAAGTCATGCCAGTCCTC
PRIMER_RIGHT_44_SEQUENCE=TGGTATTGTTTCTCACCGTCC
PRIMER_LEFT_44=720,20
PRIMER_RIGHT_44=1526,21
PRIMER_LEFT_44_TM=58.802
PRIMER_RIGHT_44_TM=59.845
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=9.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=807
PRIMER_PAIR_45_PENALTY=2.378199
PRIMER_LEFT_45_PENALTY=0.426379
PRIMER_RIGHT_45_PENALTY=1.951820
PRIMER_LEFT_45_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_45_SEQUENCE=TGTTTCTCACCGTCCATTTT
PRIMER_LEFT_45=700,20
PRIMER_RIGHT_45=1520,20
PRIMER_LEFT_45_TM=59.574
PRIMER_RIGHT_45_TM=58.048
PRIMER_LEFT_45_GC_PERCENT=60.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=7.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=821
PRIMER_PAIR_46_PENALTY=2.378199
PRIMER_LEFT_46_PENALTY=0.426379
PRIMER_RIGHT_46_PENALTY=1.951820
PRIMER_LEFT_46_SEQUENCE=CGTCTCCACCACCTAGAACC
PRIMER_RIGHT_46_SEQUENCE=TTGTTTCTCACCGTCCATTT
PRIMER_LEFT_46=700,20
PRIMER_RIGHT_46=1521,20
PRIMER_LEFT_46_TM=59.574
PRIMER_RIGHT_46_TM=58.048
PRIMER_LEFT_46_GC_PERCENT=60.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=7.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=822
PRIMER_PAIR_47_PENALTY=2.423454
PRIMER_LEFT_47_PENALTY=0.083883
PRIMER_RIGHT_47_PENALTY=2.339571
PRIMER_LEFT_47_SEQUENCE=TAAATACGGAGCAGGATGGG
PRIMER_RIGHT_47_SEQUENCE=ATCCCCAAGGAAAAATCAAGG
PRIMER_LEFT_47=868,20
PRIMER_RIGHT_47=1837,21
PRIMER_LEFT_47_TM=59.916
PRIMER_RIGHT_47_TM=61.340
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=970
PRIMER_PAIR_48_PENALTY=2.425625
PRIMER_LEFT_48_PENALTY=2.159005
PRIMER_RIGHT_48_PENALTY=0.266620
PRIMER_LEFT_48_SEQUENCE=TTAAATACGGAGCAGGATGGG
PRIMER_RIGHT_48_SEQUENCE=CAATCCCCAAGGAAAAATCA
PRIMER_LEFT_48=867,21
PRIMER_RIGHT_48=1839,20
PRIMER_LEFT_48_TM=61.159
PRIMER_RIGHT_48_TM=59.733
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=973
PRIMER_PAIR_49_PENALTY=2.449320
PRIMER_LEFT_49_PENALTY=1.293870
PRIMER_RIGHT_49_PENALTY=1.155450
PRIMER_LEFT_49_SEQUENCE=TCTCCACCACCTAGAACCTCA
PRIMER_RIGHT_49_SEQUENCE=GGTATTGTTTCTCACCGTCCA
PRIMER_LEFT_49=702,21
PRIMER_RIGHT_49=1525,21
PRIMER_LEFT_49_TM=59.706
PRIMER_RIGHT_49_TM=59.845
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=824
=
SEQUENCE_ID=MAGIC.433_4_5385099_5385716_wu-0_oy-0_437161_24528_TRUE
SEQUENCE_TARGET=950,717
SEQUENCE_TEMPLATE=GAGACACNTAAGTATGAAATGCCGAGCGTCTAGTGNTAAAGTATCAGGTACGTCTGGAAGCGTACCCNTTNCNATCTNAANCGCGGCTTGAATCTGCGCGTTTNCACAAATTCTGACNTTTAACTCATAAAATCCCATAAAGTAGACACTTCAAATGTCGTTTAAGATTACTAAGACAGTTNAAAANTTTGAAAATCTTGAAGACTTACNGGTTNNAGATNGGAGTAGGGGATCTGACCAGTACACATTTCCAGCACAGTGCACCCAAGGCTCCATATATCAGCTGGACTTCCATTGNCATCACTATCCTNTCGGTTNATNACCTGATCAAATGGTTATGTTCTATTTAGAAATAACCATACAAGTATAGCCTCGAGTATAAACATACTNACAGTTACCAACTNACCATAAACAAAAATTTAACCAAATACNTTGAAGGAAACATAATCGNNTTTTTTTAAGGTTATCANNGCCGAGAACATTGCTATTGATGTATNATATGTTGTCTATCATTGTATTCACACAATCCAAAACAATGAGAAGGATCCCAACACAATTACCTCCGGAGCCATCCAAAATAATGTTCCCNTGCAAGACATAATGNCGTTGAACTTTGACGCCTATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACATATTTGCACATTTAATGTCCCNTTGTCGCATCAGATGAGAANNNNAAGTAACANATGAAAGAAATCAGATTTTTAAGAAATACTATGACAAGAGAGTTTACTAGTCAAACCTGTGAACAAAGCCTTTATCGTGGAGATAATTCAACCCAGCAAGAATTTGTCTTGTATACAAGGAGACTACNGTGTAGCTAAGTTGGTATCTTTCGTAGAGTTTTTGAACGGATCCNTGGGTTACAAGCTCAAGAAAGATGTACAATTTTGACACGTCCTGTAAACAGNGAAAGGAGTTACCAATTGCACAACAAAATNCAACTGAGAACAAGAAAGAGAGAACANGACTACACCTTGGCTGTGCCACGATATCGCACAATATTCTGATGCTGAAGCTGACTAAGTAGTGCAATCTCNTGTAGAAAAATAAAAAATGAAAAANNTGNCAAATGTAAGGTNGATTAGCANCCTACTTTTGACNGATCAATGCAATAATGCTACAATGAAAGAAGTGATAAATGCTANGGATAACTGATGTATATGACTTTCATACCCCCTCAAGTTGTTGAATGCATTCTTGTGNCTGAATTCCCNNATCAAGAANTGAAACTTCCTTGACAGCAAAGAAGTCCCCATCTCTACATNTAACATAAACCACCAGCCAAAAAANCATATGTTGGCTCAACCAAGTATCTTACTTTAAGNTCAACAAACGTAAGCAANCAGTANGACTTACTCTGAAATGGCTTCATACACANAGGCANATGATCCTCNTCCTAGAAGTTGACCCTTCAGCCAAGACGTNATATCCTCNGGCTTACNAAAATTCTCAATCAACTTGTTACNCATCAANTTANNCTTTTCCTTAACACGTCCACTAGGACCTTCTTCGCTGAAATCCCTTTTNCGGAAACTGATGCGATCCGGGAACTCCATAGANGGNGTCATCAAGGAACAAGTCGACTCGGAACCAACNGAGGAACTAGAANAAGACATGATATAATGANGNTGAAGCTGACTAAGTAATGCAATCTCCCANAAANAATGAAAGAGAAGACAATGAGATAATGCCACAATGGTAATGCAACCATAAAANTNGCTTNGCACCGTATTTTTCANCTCATTAAACATCTNATTGTAAATAGGCATGNTGTTCTTTTCTTNCCAACTAAAGAGTGTATAATTTGATNTAAAGGNNNNNNNNNNNATCCATCAAAAANCATCAACAAAANACACAAAGAAAAANTCTCGCACTGCANCACATTTCNCAANTNAAAGTTTANTTTCCTATAAACTAAGTATCTAATTTNNAGTTCAACAAACATAAGCAACCNNTAAGACTTACTCTGAAATGGCTTCATANACAGAGGCAAATGATNCTTCTCCTAGAAGTTGACCCTTNAGCCAAGNTGTGACTGGTTCAATNTCCTCCGGATTGCNAAAATTTTCAATCAACTTGGCACGCGTCAATTTACGCTTTTCCCAAANGTGTCGACTAGGACCTTTTTCACTGAAATCCTTTTTCCGGAAACTGATGCGATCNGGGAACTCCAGAGAAGGCGTCATCAAGGAACAAGTCGACTCGGAACCAACGGAGGAACTAGAAGAAGACATGATATTATGATGCTGAANCTGACNAAGTAGTGCAATCTCCCNNANAAAATAAAAGATATAGCAATGAGATAATGCTACAGATNTTAGCACTTACCCTGAANTGCCTTCATACACANAACCNAATGATACTCGTCNCAGAAATTTCCTCTTCAGCCAAGAAGGGACGATAGANCCTNNAGAGGCATAACTNGNCGAAATAATGGATANTGTACTNNNGGAGTCAGCCTCGTTTGTAGCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.883805
PRIMER_LEFT_0_PENALTY=1.103105
PRIMER_RIGHT_0_PENALTY=2.780701
PRIMER_LEFT_0_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_0_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_0=915,21
PRIMER_RIGHT_0=1849,22
PRIMER_LEFT_0_TM=60.103
PRIMER_RIGHT_0_TM=59.219
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=5.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=935
PRIMER_PAIR_1_PENALTY=4.032171
PRIMER_LEFT_1_PENALTY=1.251471
PRIMER_RIGHT_1_PENALTY=2.780701
PRIMER_LEFT_1_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_1_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_1=878,20
PRIMER_RIGHT_1=1849,22
PRIMER_LEFT_1_TM=58.749
PRIMER_RIGHT_1_TM=59.219
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=972
PRIMER_PAIR_2_PENALTY=4.150754
PRIMER_LEFT_2_PENALTY=1.370054
PRIMER_RIGHT_2_PENALTY=2.780701
PRIMER_LEFT_2_SEQUENCE=GTCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_2_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_2=877,21
PRIMER_RIGHT_2=1849,22
PRIMER_LEFT_2_TM=59.630
PRIMER_RIGHT_2_TM=59.219
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=973
PRIMER_PAIR_3_PENALTY=4.234733
PRIMER_LEFT_3_PENALTY=1.454032
PRIMER_RIGHT_3_PENALTY=2.780701
PRIMER_LEFT_3_SEQUENCE=TGGAGATAATTCAACCCAGCA
PRIMER_RIGHT_3_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_3=905,21
PRIMER_RIGHT_3=1849,22
PRIMER_LEFT_3_TM=60.454
PRIMER_RIGHT_3_TM=59.219
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=945
PRIMER_PAIR_4_PENALTY=4.579854
PRIMER_LEFT_4_PENALTY=1.799154
PRIMER_RIGHT_4_PENALTY=2.780701
PRIMER_LEFT_4_SEQUENCE=CAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_4_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_4=916,20
PRIMER_RIGHT_4=1849,22
PRIMER_LEFT_4_TM=58.201
PRIMER_RIGHT_4_TM=59.219
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=934
PRIMER_PAIR_5_PENALTY=4.814803
PRIMER_LEFT_5_PENALTY=2.034102
PRIMER_RIGHT_5_PENALTY=2.780701
PRIMER_LEFT_5_SEQUENCE=GGAGATAATTCAACCCAGCAAG
PRIMER_RIGHT_5_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_5=906,22
PRIMER_RIGHT_5=1849,22
PRIMER_LEFT_5_TM=59.966
PRIMER_RIGHT_5_TM=59.219
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=40.909
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=5.9000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=944
PRIMER_PAIR_6_PENALTY=4.931913
PRIMER_LEFT_6_PENALTY=1.103105
PRIMER_RIGHT_6_PENALTY=3.828809
PRIMER_LEFT_6_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_6_SEQUENCE=TCGACTTGTTCCTTGATGAC
PRIMER_LEFT_6=915,21
PRIMER_RIGHT_6=1727,20
PRIMER_LEFT_6_TM=60.103
PRIMER_RIGHT_6_TM=56.171
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=813
PRIMER_PAIR_7_PENALTY=5.044420
PRIMER_LEFT_7_PENALTY=1.251471
PRIMER_RIGHT_7_PENALTY=3.792949
PRIMER_LEFT_7_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_7_SEQUENCE=GTCGACTTGTTCCTTGATGAC
PRIMER_LEFT_7=878,20
PRIMER_RIGHT_7=1728,21
PRIMER_LEFT_7_TM=58.749
PRIMER_RIGHT_7_TM=57.207
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=6.3000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=851
PRIMER_PAIR_8_PENALTY=5.080279
PRIMER_LEFT_8_PENALTY=1.251471
PRIMER_RIGHT_8_PENALTY=3.828809
PRIMER_LEFT_8_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_8_SEQUENCE=TCGACTTGTTCCTTGATGAC
PRIMER_LEFT_8=878,20
PRIMER_RIGHT_8=1727,20
PRIMER_LEFT_8_TM=58.749
PRIMER_RIGHT_8_TM=56.171
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=6.3000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=850
PRIMER_PAIR_9_PENALTY=5.170524
PRIMER_LEFT_9_PENALTY=2.389824
PRIMER_RIGHT_9_PENALTY=2.780701
PRIMER_LEFT_9_SEQUENCE=GGAGATAATTCAACCCAGCA
PRIMER_RIGHT_9_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_9=906,20
PRIMER_RIGHT_9=1849,22
PRIMER_LEFT_9_TM=57.610
PRIMER_RIGHT_9_TM=59.219
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=5.9000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=944
PRIMER_PAIR_10_PENALTY=5.246981
PRIMER_LEFT_10_PENALTY=1.454032
PRIMER_RIGHT_10_PENALTY=3.792949
PRIMER_LEFT_10_SEQUENCE=TGGAGATAATTCAACCCAGCA
PRIMER_RIGHT_10_SEQUENCE=GTCGACTTGTTCCTTGATGAC
PRIMER_LEFT_10=905,21
PRIMER_RIGHT_10=1728,21
PRIMER_LEFT_10_TM=60.454
PRIMER_RIGHT_10_TM=57.207
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=824
PRIMER_PAIR_11_PENALTY=5.282841
PRIMER_LEFT_11_PENALTY=1.454032
PRIMER_RIGHT_11_PENALTY=3.828809
PRIMER_LEFT_11_SEQUENCE=TGGAGATAATTCAACCCAGCA
PRIMER_RIGHT_11_SEQUENCE=TCGACTTGTTCCTTGATGAC
PRIMER_LEFT_11=905,21
PRIMER_RIGHT_11=1727,20
PRIMER_LEFT_11_TM=60.454
PRIMER_RIGHT_11_TM=56.171
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=823
PRIMER_PAIR_12_PENALTY=5.349165
PRIMER_LEFT_12_PENALTY=2.568464
PRIMER_RIGHT_12_PENALTY=2.780701
PRIMER_LEFT_12_SEQUENCE=AGTCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_12_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_12=876,22
PRIMER_RIGHT_12=1849,22
PRIMER_LEFT_12_TM=60.568
PRIMER_RIGHT_12_TM=59.219
PRIMER_LEFT_12_GC_PERCENT=45.455
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=5.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=974
PRIMER_PAIR_13_PENALTY=5.592103
PRIMER_LEFT_13_PENALTY=1.799154
PRIMER_RIGHT_13_PENALTY=3.792949
PRIMER_LEFT_13_SEQUENCE=CAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_13_SEQUENCE=GTCGACTTGTTCCTTGATGAC
PRIMER_LEFT_13=916,20
PRIMER_RIGHT_13=1728,21
PRIMER_LEFT_13_TM=58.201
PRIMER_RIGHT_13_TM=57.207
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=813
PRIMER_PAIR_14_PENALTY=5.626417
PRIMER_LEFT_14_PENALTY=1.103105
PRIMER_RIGHT_14_PENALTY=4.523312
PRIMER_LEFT_14_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_14_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_14=915,21
PRIMER_RIGHT_14=1695,20
PRIMER_LEFT_14_TM=60.103
PRIMER_RIGHT_14_TM=64.523
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=781
PRIMER_PAIR_15_PENALTY=5.627962
PRIMER_LEFT_15_PENALTY=1.799154
PRIMER_RIGHT_15_PENALTY=3.828809
PRIMER_LEFT_15_SEQUENCE=CAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_15_SEQUENCE=TCGACTTGTTCCTTGATGAC
PRIMER_LEFT_15=916,20
PRIMER_RIGHT_15=1727,20
PRIMER_LEFT_15_TM=58.201
PRIMER_RIGHT_15_TM=56.171
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.3000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=812
PRIMER_PAIR_16_PENALTY=5.760079
PRIMER_LEFT_16_PENALTY=1.967130
PRIMER_RIGHT_16_PENALTY=3.792949
PRIMER_LEFT_16_SEQUENCE=GGAGATAATTCAACCCAGCAA
PRIMER_RIGHT_16_SEQUENCE=GTCGACTTGTTCCTTGATGAC
PRIMER_LEFT_16=906,21
PRIMER_RIGHT_16=1728,21
PRIMER_LEFT_16_TM=59.033
PRIMER_RIGHT_16_TM=57.207
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=823
PRIMER_PAIR_17_PENALTY=5.774783
PRIMER_LEFT_17_PENALTY=1.251471
PRIMER_RIGHT_17_PENALTY=4.523312
PRIMER_LEFT_17_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_17_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_17=878,20
PRIMER_RIGHT_17=1695,20
PRIMER_LEFT_17_TM=58.749
PRIMER_RIGHT_17_TM=64.523
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=818
PRIMER_PAIR_18_PENALTY=5.795938
PRIMER_LEFT_18_PENALTY=1.967130
PRIMER_RIGHT_18_PENALTY=3.828809
PRIMER_LEFT_18_SEQUENCE=GGAGATAATTCAACCCAGCAA
PRIMER_RIGHT_18_SEQUENCE=TCGACTTGTTCCTTGATGAC
PRIMER_LEFT_18=906,21
PRIMER_RIGHT_18=1727,20
PRIMER_LEFT_18_TM=59.033
PRIMER_RIGHT_18_TM=56.171
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=6.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=822
PRIMER_PAIR_19_PENALTY=5.827051
PRIMER_LEFT_19_PENALTY=2.034102
PRIMER_RIGHT_19_PENALTY=3.792949
PRIMER_LEFT_19_SEQUENCE=GGAGATAATTCAACCCAGCAAG
PRIMER_RIGHT_19_SEQUENCE=GTCGACTTGTTCCTTGATGAC
PRIMER_LEFT_19=906,22
PRIMER_RIGHT_19=1728,21
PRIMER_LEFT_19_TM=59.966
PRIMER_RIGHT_19_TM=57.207
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=823
PRIMER_PAIR_20_PENALTY=5.862911
PRIMER_LEFT_20_PENALTY=2.034102
PRIMER_RIGHT_20_PENALTY=3.828809
PRIMER_LEFT_20_SEQUENCE=GGAGATAATTCAACCCAGCAAG
PRIMER_RIGHT_20_SEQUENCE=TCGACTTGTTCCTTGATGAC
PRIMER_LEFT_20=906,22
PRIMER_RIGHT_20=1727,20
PRIMER_LEFT_20_TM=59.966
PRIMER_RIGHT_20_TM=56.171
PRIMER_LEFT_20_GC_PERCENT=45.455
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=6.3000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=822
PRIMER_PAIR_21_PENALTY=5.893366
PRIMER_LEFT_21_PENALTY=1.370054
PRIMER_RIGHT_21_PENALTY=4.523312
PRIMER_LEFT_21_SEQUENCE=GTCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_21_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_21=877,21
PRIMER_RIGHT_21=1695,20
PRIMER_LEFT_21_TM=59.630
PRIMER_RIGHT_21_TM=64.523
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=819
PRIMER_PAIR_22_PENALTY=5.941010
PRIMER_LEFT_22_PENALTY=1.103105
PRIMER_RIGHT_22_PENALTY=4.837905
PRIMER_LEFT_22_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_22_SEQUENCE=GTGGCATTATCTCATTGTCTTCTC
PRIMER_LEFT_22=915,21
PRIMER_RIGHT_22=1838,24
PRIMER_LEFT_22_TM=60.103
PRIMER_RIGHT_22_TM=59.162
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=41.667
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=924
PRIMER_PAIR_23_PENALTY=5.977344
PRIMER_LEFT_23_PENALTY=1.454032
PRIMER_RIGHT_23_PENALTY=4.523312
PRIMER_LEFT_23_SEQUENCE=TGGAGATAATTCAACCCAGCA
PRIMER_RIGHT_23_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_23=905,21
PRIMER_RIGHT_23=1695,20
PRIMER_LEFT_23_TM=60.454
PRIMER_RIGHT_23_TM=64.523
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=791
PRIMER_PAIR_24_PENALTY=6.009111
PRIMER_LEFT_24_PENALTY=3.228411
PRIMER_RIGHT_24_PENALTY=2.780701
PRIMER_LEFT_24_SEQUENCE=GTGGAGATAATTCAACCCAGCA
PRIMER_RIGHT_24_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_24=904,22
PRIMER_RIGHT_24=1849,22
PRIMER_LEFT_24_TM=61.228
PRIMER_RIGHT_24_TM=59.219
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=5.9000
PRIMER_PAIR_24_COMPL_ANY=7.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=946
PRIMER_PAIR_25_PENALTY=6.058167
PRIMER_LEFT_25_PENALTY=1.103105
PRIMER_RIGHT_25_PENALTY=4.955063
PRIMER_LEFT_25_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_25_SEQUENCE=TTCCCGGATCGCATCAGTTT
PRIMER_LEFT_25=915,21
PRIMER_RIGHT_25=1694,20
PRIMER_LEFT_25_TM=60.103
PRIMER_RIGHT_25_TM=64.955
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=780
PRIMER_PAIR_26_PENALTY=6.089376
PRIMER_LEFT_26_PENALTY=1.251471
PRIMER_RIGHT_26_PENALTY=4.837905
PRIMER_LEFT_26_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_26_SEQUENCE=GTGGCATTATCTCATTGTCTTCTC
PRIMER_LEFT_26=878,20
PRIMER_RIGHT_26=1838,24
PRIMER_LEFT_26_TM=58.749
PRIMER_RIGHT_26_TM=59.162
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=41.667
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=961
PRIMER_PAIR_27_PENALTY=6.144111
PRIMER_LEFT_27_PENALTY=3.363410
PRIMER_RIGHT_27_PENALTY=2.780701
PRIMER_LEFT_27_SEQUENCE=TTCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_27_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_27=914,22
PRIMER_RIGHT_27=1849,22
PRIMER_LEFT_27_TM=61.363
PRIMER_RIGHT_27_TM=59.219
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=5.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=936
PRIMER_PAIR_28_PENALTY=6.146528
PRIMER_LEFT_28_PENALTY=1.103105
PRIMER_RIGHT_28_PENALTY=5.043423
PRIMER_LEFT_28_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_28_SEQUENCE=GTGGCATTATCTCATTGTCTTCTCT
PRIMER_LEFT_28=915,21
PRIMER_RIGHT_28=1838,25
PRIMER_LEFT_28_TM=60.103
PRIMER_RIGHT_28_TM=60.043
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=924
PRIMER_PAIR_29_PENALTY=6.182773
PRIMER_LEFT_29_PENALTY=2.389824
PRIMER_RIGHT_29_PENALTY=3.792949
PRIMER_LEFT_29_SEQUENCE=GGAGATAATTCAACCCAGCA
PRIMER_RIGHT_29_SEQUENCE=GTCGACTTGTTCCTTGATGAC
PRIMER_LEFT_29=906,20
PRIMER_RIGHT_29=1728,21
PRIMER_LEFT_29_TM=57.610
PRIMER_RIGHT_29_TM=57.207
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=6.3000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=823
PRIMER_PAIR_30_PENALTY=6.206533
PRIMER_LEFT_30_PENALTY=1.251471
PRIMER_RIGHT_30_PENALTY=4.955063
PRIMER_LEFT_30_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_30_SEQUENCE=TTCCCGGATCGCATCAGTTT
PRIMER_LEFT_30=878,20
PRIMER_RIGHT_30=1694,20
PRIMER_LEFT_30_TM=58.749
PRIMER_RIGHT_30_TM=64.955
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=817
PRIMER_PAIR_31_PENALTY=6.207959
PRIMER_LEFT_31_PENALTY=1.370054
PRIMER_RIGHT_31_PENALTY=4.837905
PRIMER_LEFT_31_SEQUENCE=GTCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_31_SEQUENCE=GTGGCATTATCTCATTGTCTTCTC
PRIMER_LEFT_31=877,21
PRIMER_RIGHT_31=1838,24
PRIMER_LEFT_31_TM=59.630
PRIMER_RIGHT_31_TM=59.162
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=41.667
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.7000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=962
PRIMER_PAIR_32_PENALTY=6.218632
PRIMER_LEFT_32_PENALTY=2.389824
PRIMER_RIGHT_32_PENALTY=3.828809
PRIMER_LEFT_32_SEQUENCE=GGAGATAATTCAACCCAGCA
PRIMER_RIGHT_32_SEQUENCE=TCGACTTGTTCCTTGATGAC
PRIMER_LEFT_32=906,20
PRIMER_RIGHT_32=1727,20
PRIMER_LEFT_32_TM=57.610
PRIMER_RIGHT_32_TM=56.171
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=6.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=822
PRIMER_PAIR_33_PENALTY=6.294894
PRIMER_LEFT_33_PENALTY=1.251471
PRIMER_RIGHT_33_PENALTY=5.043423
PRIMER_LEFT_33_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_33_SEQUENCE=GTGGCATTATCTCATTGTCTTCTCT
PRIMER_LEFT_33=878,20
PRIMER_RIGHT_33=1838,25
PRIMER_LEFT_33_TM=58.749
PRIMER_RIGHT_33_TM=60.043
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=961
PRIMER_PAIR_34_PENALTY=6.322466
PRIMER_LEFT_34_PENALTY=1.799154
PRIMER_RIGHT_34_PENALTY=4.523312
PRIMER_LEFT_34_SEQUENCE=CAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_34_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_34=916,20
PRIMER_RIGHT_34=1695,20
PRIMER_LEFT_34_TM=58.201
PRIMER_RIGHT_34_TM=64.523
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=780
PRIMER_PAIR_35_PENALTY=6.325116
PRIMER_LEFT_35_PENALTY=1.370054
PRIMER_RIGHT_35_PENALTY=4.955063
PRIMER_LEFT_35_SEQUENCE=GTCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_35_SEQUENCE=TTCCCGGATCGCATCAGTTT
PRIMER_LEFT_35=877,21
PRIMER_RIGHT_35=1694,20
PRIMER_LEFT_35_TM=59.630
PRIMER_RIGHT_35_TM=64.955
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=818
PRIMER_PAIR_36_PENALTY=6.338968
PRIMER_LEFT_36_PENALTY=3.558267
PRIMER_RIGHT_36_PENALTY=2.780701
PRIMER_LEFT_36_SEQUENCE=TAGTCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_36_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_36=875,23
PRIMER_RIGHT_36=1849,22
PRIMER_LEFT_36_TM=60.558
PRIMER_RIGHT_36_TM=59.219
PRIMER_LEFT_36_GC_PERCENT=43.478
PRIMER_RIGHT_36_GC_PERCENT=40.909
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=5.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=975
PRIMER_PAIR_37_PENALTY=6.409095
PRIMER_LEFT_37_PENALTY=1.454032
PRIMER_RIGHT_37_PENALTY=4.955063
PRIMER_LEFT_37_SEQUENCE=TGGAGATAATTCAACCCAGCA
PRIMER_RIGHT_37_SEQUENCE=TTCCCGGATCGCATCAGTTT
PRIMER_LEFT_37=905,21
PRIMER_RIGHT_37=1694,20
PRIMER_LEFT_37_TM=60.454
PRIMER_RIGHT_37_TM=64.955
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=790
PRIMER_PAIR_38_PENALTY=6.413477
PRIMER_LEFT_38_PENALTY=1.370054
PRIMER_RIGHT_38_PENALTY=5.043423
PRIMER_LEFT_38_SEQUENCE=GTCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_38_SEQUENCE=GTGGCATTATCTCATTGTCTTCTCT
PRIMER_LEFT_38=877,21
PRIMER_RIGHT_38=1838,25
PRIMER_LEFT_38_TM=59.630
PRIMER_RIGHT_38_TM=60.043
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=962
PRIMER_PAIR_39_PENALTY=6.452472
PRIMER_LEFT_39_PENALTY=3.671771
PRIMER_RIGHT_39_PENALTY=2.780701
PRIMER_LEFT_39_SEQUENCE=GAGATAATTCAACCCAGCAAGA
PRIMER_RIGHT_39_SEQUENCE=GCATTACCATTGTGGCATTATC
PRIMER_LEFT_39=907,22
PRIMER_RIGHT_39=1849,22
PRIMER_LEFT_39_TM=58.328
PRIMER_RIGHT_39_TM=59.219
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=5.9000
PRIMER_PAIR_39_COMPL_ANY=7.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=943
PRIMER_PAIR_40_PENALTY=6.490442
PRIMER_LEFT_40_PENALTY=1.967130
PRIMER_RIGHT_40_PENALTY=4.523312
PRIMER_LEFT_40_SEQUENCE=GGAGATAATTCAACCCAGCAA
PRIMER_RIGHT_40_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_40=906,21
PRIMER_RIGHT_40=1695,20
PRIMER_LEFT_40_TM=59.033
PRIMER_RIGHT_40_TM=64.523
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=790
PRIMER_PAIR_41_PENALTY=6.557414
PRIMER_LEFT_41_PENALTY=2.034102
PRIMER_RIGHT_41_PENALTY=4.523312
PRIMER_LEFT_41_SEQUENCE=GGAGATAATTCAACCCAGCAAG
PRIMER_RIGHT_41_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_41=906,22
PRIMER_RIGHT_41=1695,20
PRIMER_LEFT_41_TM=59.966
PRIMER_RIGHT_41_TM=64.523
PRIMER_LEFT_41_GC_PERCENT=45.455
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=790
PRIMER_PAIR_42_PENALTY=6.637059
PRIMER_LEFT_42_PENALTY=1.799154
PRIMER_RIGHT_42_PENALTY=4.837905
PRIMER_LEFT_42_SEQUENCE=CAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_42_SEQUENCE=GTGGCATTATCTCATTGTCTTCTC
PRIMER_LEFT_42=916,20
PRIMER_RIGHT_42=1838,24
PRIMER_LEFT_42_TM=58.201
PRIMER_RIGHT_42_TM=59.162
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=41.667
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=923
PRIMER_PAIR_43_PENALTY=6.754216
PRIMER_LEFT_43_PENALTY=1.799154
PRIMER_RIGHT_43_PENALTY=4.955063
PRIMER_LEFT_43_SEQUENCE=CAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_43_SEQUENCE=TTCCCGGATCGCATCAGTTT
PRIMER_LEFT_43=916,20
PRIMER_RIGHT_43=1694,20
PRIMER_LEFT_43_TM=58.201
PRIMER_RIGHT_43_TM=64.955
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=779
PRIMER_PAIR_44_PENALTY=6.789514
PRIMER_LEFT_44_PENALTY=1.103105
PRIMER_RIGHT_44_PENALTY=5.686409
PRIMER_LEFT_44_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_44_SEQUENCE=GTGGCATTATCTCATTGTCTTC
PRIMER_LEFT_44=915,21
PRIMER_RIGHT_44=1838,22
PRIMER_LEFT_44_TM=60.103
PRIMER_RIGHT_44_TM=56.314
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=924
PRIMER_PAIR_45_PENALTY=6.830985
PRIMER_LEFT_45_PENALTY=1.103105
PRIMER_RIGHT_45_PENALTY=5.727880
PRIMER_LEFT_45_SEQUENCE=TCAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_45_SEQUENCE=GCATTACCATTGTGGCATTATCTC
PRIMER_LEFT_45=915,21
PRIMER_RIGHT_45=1849,24
PRIMER_LEFT_45_TM=60.103
PRIMER_RIGHT_45_TM=61.728
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=41.667
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.3000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=935
PRIMER_PAIR_46_PENALTY=6.842577
PRIMER_LEFT_46_PENALTY=1.799154
PRIMER_RIGHT_46_PENALTY=5.043423
PRIMER_LEFT_46_SEQUENCE=CAACCCAGCAAGAATTTGTC
PRIMER_RIGHT_46_SEQUENCE=GTGGCATTATCTCATTGTCTTCTCT
PRIMER_LEFT_46=916,20
PRIMER_RIGHT_46=1838,25
PRIMER_LEFT_46_TM=58.201
PRIMER_RIGHT_46_TM=60.043
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=923
PRIMER_PAIR_47_PENALTY=6.913136
PRIMER_LEFT_47_PENALTY=2.389824
PRIMER_RIGHT_47_PENALTY=4.523312
PRIMER_LEFT_47_SEQUENCE=GGAGATAATTCAACCCAGCA
PRIMER_RIGHT_47_SEQUENCE=GTTCCCGGATCGCATCAGTT
PRIMER_LEFT_47=906,20
PRIMER_RIGHT_47=1695,20
PRIMER_LEFT_47_TM=57.610
PRIMER_RIGHT_47_TM=64.523
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=790
PRIMER_PAIR_48_PENALTY=6.922192
PRIMER_LEFT_48_PENALTY=1.967130
PRIMER_RIGHT_48_PENALTY=4.955063
PRIMER_LEFT_48_SEQUENCE=GGAGATAATTCAACCCAGCAA
PRIMER_RIGHT_48_SEQUENCE=TTCCCGGATCGCATCAGTTT
PRIMER_LEFT_48=906,21
PRIMER_RIGHT_48=1694,20
PRIMER_LEFT_48_TM=59.033
PRIMER_RIGHT_48_TM=64.955
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=789
PRIMER_PAIR_49_PENALTY=6.937880
PRIMER_LEFT_49_PENALTY=1.251471
PRIMER_RIGHT_49_PENALTY=5.686409
PRIMER_LEFT_49_SEQUENCE=TCAAACCTGTGAACAAAGCC
PRIMER_RIGHT_49_SEQUENCE=GTGGCATTATCTCATTGTCTTC
PRIMER_LEFT_49=878,20
PRIMER_RIGHT_49=1838,22
PRIMER_LEFT_49_TM=58.749
PRIMER_RIGHT_49_TM=56.314
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=961
=
SEQUENCE_ID=MAGIC.287_4_7944770_7945176_tsu-0_mt-0_139467_164195_TRUE
SEQUENCE_TARGET=950,506
SEQUENCE_TEMPLATE=GATGNAGNCNNCGAAATANAAANAAGAGGCAAAAGTATTTAGGANAAAAATGTAAANGAANACTTTTATATTTCGAGTTAATTTTCAATGTACATACTGTTCAGATCCAATCTAACAACTCTNTTACCTCTANTTAAACCACNTAACACAGCTACCTCATGGACCATGGTGANTCAAAATTGGTNATATAAAGGTGTGTTTACTTTTGGTGATTTGTTTTTAGCTATTAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATACNATTTTGTTTTGATTGANNNNNNNNTANNANTCAAAATANTATTACANAAAAAAAATTATAAGANAAANTAAANGGTNAATTGNAAAACTAGNCATATTTNATAANTTAATTAAGAATATANNATNATATANATATAAATTAANAAAATAGNATATAAGNGTTGGGAAAAGANAATTTTGNTGTGNAATAGTTGATGAGAGATGATTTANNAACANTTCATTTANTTTTCANCTATTTGTATGTTTTTGTTATATATNTTTTCCAAAAAATNGATTCTAATTATGAAATTTATNANATAAAAATTTCTGNTTTTTTCTACATACAACATAGTTTAAATTTTTAAAATCGGANTTGTATTTATAAAATANNAAAAAATNTTTGTAAGTAAATTTATATTGTACTATTTATNATGTATANAAATAGAATGTTGTACTATACTATTTTCATAAATCATTCGTAATTTAGNTTTAATTTTAGTATAAAACAAAATATTTTAAATTAAATAAAGTAATAGNGGTGGTAGTGGTGGTGANATAGTAGTTNATGTTGCAATATTANCTACGATAATCGTAAAAGATAAAACATANACGATTTAAATTANCNANTNAAANCGATATGATCNCCATTAAAAAACTATANCNTAAGAGATACAATATTGGGAACNCAAGCAAGCAANTTAACTGNNNNNNNNNNNNNNNNNNNNTTGTAGTGACGACAATACNGTTATAGCAGTGAGCCACTGNTAAGGATAATNGTGANNTGGTAGTAGAGGTAATGATGTAATAATATAGGTGATAATTGTATNATGAATTTTANTATGTGTAANNTTTTTTTTTGTNNNNNTANAANAGAAAGTACATATAGAAAATAGAAGTANNNNACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATCATNTNNTTTTTGTTTTGATNNTANTTTNTTAATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTNNANNAAATATAATTTCNTNCNNATTTTTTNNNNNNNNNNNNNNNTAANNTATNNNNNNNNNNNTATCAAGGCAATTAGATGGTGATATANGTCTAAGAATTTGAAGTAANGTAAATGGTTAAACGTCCANCTACGAAATTCGTGTAATTAATTATAATATACCTTAACTGCCTACCAAATANTAAATTATAGTGCAACTTTACTAAAGTTTACATANGTTCGAGAAGTCATTAGCATTTGACNTATTAGGATTTCATTTTACACAAGCTTTCGAGAAGATAAACATGTTTGACTTGTATACGGCTGTTCAACGACTCTTGACGAAAACTCAATTTTTNNTATTTCAAATTNCTTGAAAATCTGATGTAAANGGGCAACAACTACACGATATGATTCAAACTACTATCAATTTTCTTNNNNNNNNNNNAAAANAAAAAAAANANNNNNNACTAGACNNNNNNNNATNNNNNNNNTGTNTACATTATTTTCTAAAACAAATGTCTAGACATTNTAGTATTTTNTAAACACTTACACANCACCANATGTTTGTCTTAACCCCGACTAAAAAGGACATATGTGTTGTTAATGCTGGATCACCGGTGGTTTTGTTAGTNTCTACCCTTATTTGTNTGGAAAAANACTGCCATAGCCCATAAGGTAACTTATAAGGACATCAAAGCTTTTGGTGTTTGACTGCTTAAATGTTCAGTCTCCAGATTCCAGCACATGCATTTCTNGAATACTTGACTNATGGTGAAGCTAGCATTTTTTAATAACTTTGNCNTACCAATCATGGTGGTTGGCGGANTTAATCATGGTGGTAAGCGAGAGACTTTATTNTATNCAAAANAAAAATGAGAGACNTAAAATTAGAACTATTTNTAGTTNNNATAGTTATATTTTATTATTTTGTCTCAATTATTCTCCCCGCC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_6737858_6737869_hi-0_ler-0_146126_155255_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATCGCATAATCACGAAGAGCCTGCAAAATTACNCACTCGCAGAAAATATAACTAGTTAGCTAAAACANTAGAAAGACTATCNGAGAGAATAATAGAANTTTTTTTNTNCAGAAAAAANCAGTTTCACAGACGAATCATNANCCTCCACATCACNNGANANTGGGTATCAAAANNATCATTACACTNNNNNNNNATNAGGCCTAAATGNCTTGTCTNNNNNNNNAAAAANTTATTTATGNCTNTTNATANTNTAANNNNATCANNTANGTACATGNTCTTNGNNNNNGTTACTAATATTTTNTTTNGAATTTTGANTATTATTTTTGAAATATTACCATATAGCCAATTTTTAAAAACATGTNCTAGACCAANACTAGTCTAAGCACGGCACTAGTCCAACCTAAACTTGCTGCTTTGATATCATTCTCCAGGATGCATCAGTTTACATTTGATATCATACTCCNGAAAGNGAAGAAGATCAAACCTTNCTGCNAGTCCAGAAGAAGGATTGATCTGCGACTTTCCGACTTGAGGTTCTTGNCACTCTTTGGNCTTCTTANCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAATNTAATTGAGTTTCNTCNNNNNNNNNNNNCTCNCTCTCTCTCTCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTAATNCTCCATAGCTTCTTNGAATTTGAATTGCTNNNNNTGAATNATAATTCCATCATCTCTCGCTTTANTTTNAAAGANGAATAAGAAACAATTNAAATTTTTAATTGATTCTAATTANATAANATTANAATNNTTGNNTTNCNNATNTAGTNTNCCNTNANGGTTTAGCNNNNNCCCATCAAAAAGNNNTTAACTGATTTTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_12882099_12882321_oy-0_can-0_1769881_62919_TRUE
SEQUENCE_TARGET=950,322
SEQUENCE_TEMPLATE=TCGATGAATAACTAATGTTTTGCCTTATNATTTGCATAAAANNTATATGAAGCCTNATCACAAACTTTCTATGTAACANCTTGNCAGTTGTAAACNTGAATGGATGTATTAGCTCAAGCTCTTNNATATGAAAANANGTTCTTGATNATCAACTTCATCTATGGTTANCTTGGAAANAACNTCAANANTAAACATATATTTNTGCNTCNAAAATGGATGTTTAACTNTTGGNAAANAAAGTTTNATGAATANGTCATAAANTTAAATGTGNCAATGTCNTTTTGTTGCTTGACTTNTACACAGNNAAGNNGAATACCNGANCTTAGNAATTAGAGNTTTAAAGTTGTANATCANAAGNTAGATTTTNATTTTNTTGGNGACAAAANAGTTNCCCAATATGGTATTCGATTACAATGTTAAAGAAGTATGNGATGATTGTAGGGACATTCTCTTAAAANTTCTNNCACTTGTTATATTTGAAAGAGGNACTGATCCAAGGAACANATNATGATCTTTTTTAAGGTTGTTGTTTGTATNNAGACGCCAAAAATTGTTTTACTCGNTAGGACACTCTACCAAAACAACATAAGGGNNTTTTTTTTAAAACACTATTGNTGTTGTTTTTCTTAAGTTTCTACAACATATCCTACGTAGGAAAAATATCATAAAATTCTTGAACATTGATGNTGATACTGGNAAAAAAAATTGTAANAAGATTTTNTTGNCAAGGTTCTAATNATNGGGACAAGATCGGTTANTTCACCGAACTAATAATCTTNACAANTAANGTTATCTACAATAAGTTTNTTNTTNAAGTGTAGTGATTGNTGGACTTCCTAGTTTTAAAACCAACATCAAANCAAAGGTCNACATGTGAGATTAATAATTTTGAAAGGAAAGAGTTTCATAAACCTAGTCTNANNTNAANAAAATAACGCTTNNCCAGCCGTTATNTGCTTTTTCAACNTGGGTAAAACCTANTCTAATTNGAGGNTNAAACNTAGTCTAATTTGAACTTGANNAGAATCAAGCTTTAGTAAAATATATTNTATNANNNNNNNNNNNAATATTGTGTAAANTTTGATTTTTCTATATATTCTAAATATTNTCNTNNNNAGTAGATNTAAAATCTTAATGAATTTCCAANTTCAACGGATATCCAGACATCAAACATCCAGTAATGACAGAATGGANATATANTAAAAAACTCANTATCTGTATGNAATAGATTAGAAATTACANATAAATCTTGAATATNCAATCTGTGTCCACCGTTAATATATATAAAGCTTCTATACAATAANTAAAAATATTGGCAATTAGAAGAAAAGAAGACTAACAANNNNTTGTGCCAAAAGTACATTACACTTTTTTTAAAAGACAATAAAATATAAATTAATTCGATTGATTGAGAAGTTGCATCAATTTTCTAGTGATTATTTTGNAGCAATTGTTTTTCAAANTAGTGAATATGAAATATATAAAATATTTAACTTAATTTACAATTTGCNNNNNNNNNNNNNNNNTCTAATTAAAAANNNNANAATTGTNCATACACCGATTGAAAACAACCCANTAAAAGTAAAATAAGAAATCAACAAATAAGACTAACCTTTAATTATGACATCGTTGTTANCNCGTATATACGAAGATATAAGATAGTAATCATCGCACCATTTATGTTAACCTTGGCNATGTCNCCCGTCAAACAAGATATCGGAAACTATCTCCTTAGCGATAGTAGTGTGATATTTCTTTTCAAGGGTAGTCNTCTCAAACTCAAANTTTTACAATANNNTTTGCTCTCGACCAATANGCCATCTCAANTTTCTTTTTGACCATTGCGACCCTTANTCGNCCNATGAGAGAACTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_9624688_9624784_oy-0_sf-2_10903_2801_TRUE
SEQUENCE_TARGET=950,196
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNAATATCANGCGNTTAANTTTTGTTTNTCNCNATTNNNTTTNTTATNATAATNNNNNNNNNNNNGTATNTAGGTGATATGANAAAAANATAAAAAANGTNAATGANAANAAAGNTGNTTTTAAANTATANTANNNGTTGATTAANAGTGGTTTTACNCANAAATAGAGAANTGAGAGATTATTCGAAGTTTTAANANGTTTGAANAAAATTGTTTGTACATGTTGAAAAANCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4754100_4754231_zu-0_wu-0_14475_9636_TRUE
SEQUENCE_TARGET=950,231
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14563079_14563341_ler-0_zu-0_2453_4508_TRUE
SEQUENCE_TARGET=950,362
SEQUENCE_TEMPLATE=ATCCTCCGGTGCAGACAGAAGAACATGCCTTNGTGCATTGTTCAACCGCTTCACTCAATACTTTACTTGNGTCTAGTCATGGAAACAAAACAATATTACTTTGAATTATTCCTTTATCTTNAAAAAATGTNNNTAATAANANNAAAAAAANGAAAANAATAATAATNTATAGTGAACTTGTTTTACCAAAGTTTTGGAGAGTGGTCAAGGCACCACNCACAGAGGATGCACACCCNAGCTTGCAATATTCATTGACAGCATCACCTAAGGCAAAAATCAAGATACATGCNAATTANTTGCATCACANAATGAAATTTCCATCCATCTTATAACAAATATAACTTAATAGAAAAANTAGATATATACCAGAATTTTCGAGAATGTCATTAGTATATCCCGGAGGACATATCGTCTGAGATGTATTTTTGCATTTACTCTTTAATAGACAATAAAATTGGGATGACACGGAAAGCATGCATACAAAATACACAGATCTATCATCCTTGGTAGGACAGCAGATTTTTGCTTCTACTTGAATCTGTGCTAAGACAAGACTCATTATNAGCAGACTTGAGATCACAGTTTTGCCTTCCATCTTTTGATTGATTACTTGTTTGATCACAAAATGTACGTAGACAAAACTCTTAGTGATAACTATAAGCTTATCTTGATGAATTTGTGATGAAGCAACACTCCGTTAAATACTAGTTCTTCTACAATTTGCATCCACGAATTTCATTNATTTAATGCTCTATNAAGTCTACTCAGTGGCTACNACATATNGATGAATCTTTAATGGTGTTCGTCAGGGGAGATNGCCATGCAACAAGATTGGCTTAAATACTAGTTGTTCGACNAATTTGCATCCACAAAATTNATTTATNTAAAGCTCTNTTATCTCTACTCAGTGGCTATCACATATACTGCGCCCACTCCCATGTCAGTTTCTAGGGGGTGTGGGGGAGGTGGGGGCAAAAGCCATTGCCAGGGTGCATGGCAANTTTNTNNNAAAAAANNTTTAAGTAAAAGAGNNNNAAAAAAAAAAAAAAAATCGTGTGAAAAGGATATGTAAATTTAGTTTTNCCTAGGGTCTAAAAACATATTGAGACGGGACTNCCACCATAATAAATCNTTAAAACTAATGGCGNTTGTCTAGGGAGATGGCCATGAAACAAGATTAGCTTAAATATACTAGTTGTATTAGAACTTGCCACCAAATACTTCATATTATAACTTCATCACATGCACGCTCTACAGAGTCTNTCTCANAGTTGTAACTTGTAAAACTTCTCGAGAAGCGAGGTTTGAGAACACTTGCATNACGTGATAATGTTTGCTGCGAANGTATTTTCTTAGATAAATTTGAAGAGGATGATGAGACAAATNAAGAGGATATNATGGAAGCGNNTCAACTCAAACGATATATTTCAAAAATACCNTTNNNNNNNNNNNNATTTTGGNGCATTATTTATTCTTTNTATTCAGATATTTTGGTGCGTTNTTAATANTNNNNTTTTGAAAAGAAACACACAATCNTTCCAAAATTTNTAGANAATGTATCNNCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAGGGAACTTGGCCNNTTNANTATATNNTTATNTTTNTAGACAGGTGAAATATCTTCNCATGAATTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTTTTTGCTTGGTATCCAAAGAAGTTGCAGGTATGATTCGACCCTAGGACTTTGCCATATTCTCCTTTAAAATCTCACCCAGGTAAATTAATCGGTGNAAGTAACACATTGAGTTTAGAATTATTTTTGNTCAGTCAAAATATATCTAAAAACNTTAAACAATATCTAATAAATCAGTATAGTGAAAATATAGGTAATNACCTAGATTAGAGTCACTCCACATATCACNT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.635230
PRIMER_LEFT_0_PENALTY=0.015375
PRIMER_RIGHT_0_PENALTY=2.619855
PRIMER_LEFT_0_SEQUENCE=ACATATACTGCGCCCACTCC
PRIMER_RIGHT_0_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_0=916,20
PRIMER_RIGHT_0=1342,20
PRIMER_LEFT_0_TM=59.985
PRIMER_RIGHT_0_TM=57.380
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=427
PRIMER_PAIR_1_PENALTY=2.789600
PRIMER_LEFT_1_PENALTY=0.169745
PRIMER_RIGHT_1_PENALTY=2.619855
PRIMER_LEFT_1_SEQUENCE=AAATTGGGATGACACGGAAA
PRIMER_RIGHT_1_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_1=451,20
PRIMER_RIGHT_1=1342,20
PRIMER_LEFT_1_TM=60.170
PRIMER_RIGHT_1_TM=57.380
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=892
PRIMER_PAIR_2_PENALTY=2.789600
PRIMER_LEFT_2_PENALTY=0.169745
PRIMER_RIGHT_2_PENALTY=2.619855
PRIMER_LEFT_2_SEQUENCE=AAAATTGGGATGACACGGAA
PRIMER_RIGHT_2_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_2=450,20
PRIMER_RIGHT_2=1342,20
PRIMER_LEFT_2_TM=60.170
PRIMER_RIGHT_2_TM=57.380
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=893
PRIMER_PAIR_3_PENALTY=2.829514
PRIMER_LEFT_3_PENALTY=0.209659
PRIMER_RIGHT_3_PENALTY=2.619855
PRIMER_LEFT_3_SEQUENCE=AATTGGGATGACACGGAAAG
PRIMER_RIGHT_3_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_3=452,20
PRIMER_RIGHT_3=1342,20
PRIMER_LEFT_3_TM=59.790
PRIMER_RIGHT_3_TM=57.380
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=891
PRIMER_PAIR_4_PENALTY=2.917648
PRIMER_LEFT_4_PENALTY=0.297793
PRIMER_RIGHT_4_PENALTY=2.619855
PRIMER_LEFT_4_SEQUENCE=CGGAAAGCATGCATACAAAA
PRIMER_RIGHT_4_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_4=465,20
PRIMER_RIGHT_4=1342,20
PRIMER_LEFT_4_TM=59.702
PRIMER_RIGHT_4_TM=57.380
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=878
PRIMER_PAIR_5_PENALTY=2.982844
PRIMER_LEFT_5_PENALTY=0.362989
PRIMER_RIGHT_5_PENALTY=2.619855
PRIMER_LEFT_5_SEQUENCE=CCCACTCCCATGTCAGTTTC
PRIMER_RIGHT_5_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_5=928,20
PRIMER_RIGHT_5=1342,20
PRIMER_LEFT_5_TM=60.363
PRIMER_RIGHT_5_TM=57.380
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=415
PRIMER_PAIR_6_PENALTY=3.327182
PRIMER_LEFT_6_PENALTY=0.707326
PRIMER_RIGHT_6_PENALTY=2.619855
PRIMER_LEFT_6_SEQUENCE=TGATGAAGCAACACTCCGTT
PRIMER_RIGHT_6_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_6=679,20
PRIMER_RIGHT_6=1342,20
PRIMER_LEFT_6_TM=59.293
PRIMER_RIGHT_6_TM=57.380
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=664
PRIMER_PAIR_7_PENALTY=3.364780
PRIMER_LEFT_7_PENALTY=0.744925
PRIMER_RIGHT_7_PENALTY=2.619855
PRIMER_LEFT_7_SEQUENCE=TTAATGGTGTTCGTCAGGGG
PRIMER_RIGHT_7_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_7=792,20
PRIMER_RIGHT_7=1342,20
PRIMER_LEFT_7_TM=60.745
PRIMER_RIGHT_7_TM=57.380
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=10.9000
PRIMER_RIGHT_7_END_STABILITY=6.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=551
PRIMER_PAIR_8_PENALTY=3.379009
PRIMER_LEFT_8_PENALTY=0.759154
PRIMER_RIGHT_8_PENALTY=2.619855
PRIMER_LEFT_8_SEQUENCE=TCATCCTTGGTAGGACAGCA
PRIMER_RIGHT_8_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_8=497,20
PRIMER_RIGHT_8=1342,20
PRIMER_LEFT_8_TM=59.241
PRIMER_RIGHT_8_TM=57.380
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.3000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=846
PRIMER_PAIR_9_PENALTY=3.434187
PRIMER_LEFT_9_PENALTY=0.814332
PRIMER_RIGHT_9_PENALTY=2.619855
PRIMER_LEFT_9_SEQUENCE=ACGGAAAGCATGCATACAAA
PRIMER_RIGHT_9_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_9=464,20
PRIMER_RIGHT_9=1342,20
PRIMER_LEFT_9_TM=59.186
PRIMER_RIGHT_9_TM=57.380
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=879
PRIMER_PAIR_10_PENALTY=3.651625
PRIMER_LEFT_10_PENALTY=1.031770
PRIMER_RIGHT_10_PENALTY=2.619855
PRIMER_LEFT_10_SEQUENCE=CCATGCAACAAGATTGGCTT
PRIMER_RIGHT_10_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_10=818,20
PRIMER_RIGHT_10=1342,20
PRIMER_LEFT_10_TM=61.032
PRIMER_RIGHT_10_TM=57.380
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=525
PRIMER_PAIR_11_PENALTY=3.791302
PRIMER_LEFT_11_PENALTY=1.171447
PRIMER_RIGHT_11_PENALTY=2.619855
PRIMER_LEFT_11_SEQUENCE=GTGATGAAGCAACACTCCGTT
PRIMER_RIGHT_11_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_11=678,21
PRIMER_RIGHT_11=1342,20
PRIMER_LEFT_11_TM=60.171
PRIMER_RIGHT_11_TM=57.380
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.9000
PRIMER_RIGHT_11_END_STABILITY=6.3000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=665
PRIMER_PAIR_12_PENALTY=3.802118
PRIMER_LEFT_12_PENALTY=1.182263
PRIMER_RIGHT_12_PENALTY=2.619855
PRIMER_LEFT_12_SEQUENCE=TAAAATTGGGATGACACGGA
PRIMER_RIGHT_12_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_12=449,20
PRIMER_RIGHT_12=1342,20
PRIMER_LEFT_12_TM=58.818
PRIMER_RIGHT_12_TM=57.380
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=6.3000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=894
PRIMER_PAIR_13_PENALTY=3.881469
PRIMER_LEFT_13_PENALTY=1.261614
PRIMER_RIGHT_13_PENALTY=2.619855
PRIMER_LEFT_13_SEQUENCE=CATCCTTGGTAGGACAGCAGA
PRIMER_RIGHT_13_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_13=498,21
PRIMER_RIGHT_13=1342,20
PRIMER_LEFT_13_TM=60.262
PRIMER_RIGHT_13_TM=57.380
PRIMER_LEFT_13_GC_PERCENT=52.381
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=845
PRIMER_PAIR_14_PENALTY=3.892972
PRIMER_LEFT_14_PENALTY=1.273117
PRIMER_RIGHT_14_PENALTY=2.619855
PRIMER_LEFT_14_SEQUENCE=GTGATGAAGCAACACTCCGT
PRIMER_RIGHT_14_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_14=678,20
PRIMER_RIGHT_14=1342,20
PRIMER_LEFT_14_TM=58.727
PRIMER_RIGHT_14_TM=57.380
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=6.3000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=665
PRIMER_PAIR_15_PENALTY=3.894119
PRIMER_LEFT_15_PENALTY=1.274264
PRIMER_RIGHT_15_PENALTY=2.619855
PRIMER_LEFT_15_SEQUENCE=TCACAGTTTTGCCTTCCATCT
PRIMER_RIGHT_15_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_15=576,21
PRIMER_RIGHT_15=1342,20
PRIMER_LEFT_15_TM=59.726
PRIMER_RIGHT_15_TM=57.380
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=6.3000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=767
PRIMER_PAIR_16_PENALTY=3.894119
PRIMER_LEFT_16_PENALTY=1.274264
PRIMER_RIGHT_16_PENALTY=2.619855
PRIMER_LEFT_16_SEQUENCE=AGATCACAGTTTTGCCTTCCA
PRIMER_RIGHT_16_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_16=573,21
PRIMER_RIGHT_16=1342,20
PRIMER_LEFT_16_TM=59.726
PRIMER_RIGHT_16_TM=57.380
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=770
PRIMER_PAIR_17_PENALTY=3.916307
PRIMER_LEFT_17_PENALTY=1.296452
PRIMER_RIGHT_17_PENALTY=2.619855
PRIMER_LEFT_17_SEQUENCE=TAATGGTGTTCGTCAGGGGA
PRIMER_RIGHT_17_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_17=793,20
PRIMER_RIGHT_17=1342,20
PRIMER_LEFT_17_TM=61.296
PRIMER_RIGHT_17_TM=57.380
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=10.9000
PRIMER_RIGHT_17_END_STABILITY=6.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=550
PRIMER_PAIR_18_PENALTY=3.922805
PRIMER_LEFT_18_PENALTY=1.302950
PRIMER_RIGHT_18_PENALTY=2.619855
PRIMER_LEFT_18_SEQUENCE=TCACAGTTTTGCCTTCCATC
PRIMER_RIGHT_18_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_18=576,20
PRIMER_RIGHT_18=1342,20
PRIMER_LEFT_18_TM=58.697
PRIMER_RIGHT_18_TM=57.380
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=6.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=767
PRIMER_PAIR_19_PENALTY=3.922805
PRIMER_LEFT_19_PENALTY=1.302950
PRIMER_RIGHT_19_PENALTY=2.619855
PRIMER_LEFT_19_SEQUENCE=GATCACAGTTTTGCCTTCCA
PRIMER_RIGHT_19_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_19=574,20
PRIMER_RIGHT_19=1342,20
PRIMER_LEFT_19_TM=58.697
PRIMER_RIGHT_19_TM=57.380
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=769
PRIMER_PAIR_20_PENALTY=4.055101
PRIMER_LEFT_20_PENALTY=1.435246
PRIMER_RIGHT_20_PENALTY=2.619855
PRIMER_LEFT_20_SEQUENCE=ATCATCCTTGGTAGGACAGCA
PRIMER_RIGHT_20_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_20=496,21
PRIMER_RIGHT_20=1342,20
PRIMER_LEFT_20_TM=59.565
PRIMER_RIGHT_20_TM=57.380
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=6.3000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=847
PRIMER_PAIR_21_PENALTY=4.139926
PRIMER_LEFT_21_PENALTY=1.520071
PRIMER_RIGHT_21_PENALTY=2.619855
PRIMER_LEFT_21_SEQUENCE=CTTTAATGGTGTTCGTCAGGG
PRIMER_RIGHT_21_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_21=790,21
PRIMER_RIGHT_21=1342,20
PRIMER_LEFT_21_TM=59.480
PRIMER_RIGHT_21_TM=57.380
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=6.3000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=553
PRIMER_PAIR_22_PENALTY=4.145323
PRIMER_LEFT_22_PENALTY=1.525468
PRIMER_RIGHT_22_PENALTY=2.619855
PRIMER_LEFT_22_SEQUENCE=TTTAATGGTGTTCGTCAGGG
PRIMER_RIGHT_22_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_22=791,20
PRIMER_RIGHT_22=1342,20
PRIMER_LEFT_22_TM=58.475
PRIMER_RIGHT_22_TM=57.380
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=6.3000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=552
PRIMER_PAIR_23_PENALTY=4.391398
PRIMER_LEFT_23_PENALTY=1.771543
PRIMER_RIGHT_23_PENALTY=2.619855
PRIMER_LEFT_23_SEQUENCE=CACAGTTTTGCCTTCCATCTT
PRIMER_RIGHT_23_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_23=577,21
PRIMER_RIGHT_23=1342,20
PRIMER_LEFT_23_TM=59.228
PRIMER_RIGHT_23_TM=57.380
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=6.3000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=766
PRIMER_PAIR_24_PENALTY=4.574971
PRIMER_LEFT_24_PENALTY=1.955116
PRIMER_RIGHT_24_PENALTY=2.619855
PRIMER_LEFT_24_SEQUENCE=ATCACAGTTTTGCCTTCCATC
PRIMER_RIGHT_24_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_24=575,21
PRIMER_RIGHT_24=1342,20
PRIMER_LEFT_24_TM=59.045
PRIMER_RIGHT_24_TM=57.380
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.1000
PRIMER_RIGHT_24_END_STABILITY=6.3000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=768
PRIMER_PAIR_25_PENALTY=4.624214
PRIMER_LEFT_25_PENALTY=2.004359
PRIMER_RIGHT_25_PENALTY=2.619855
PRIMER_LEFT_25_SEQUENCE=ATCACAGTTTTGCCTTCCATCT
PRIMER_RIGHT_25_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_25=575,22
PRIMER_RIGHT_25=1342,20
PRIMER_LEFT_25_TM=60.004
PRIMER_RIGHT_25_TM=57.380
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=6.3000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=768
PRIMER_PAIR_26_PENALTY=4.703752
PRIMER_LEFT_26_PENALTY=2.083897
PRIMER_RIGHT_26_PENALTY=2.619855
PRIMER_LEFT_26_SEQUENCE=AAATTGGGATGACACGGAAAG
PRIMER_RIGHT_26_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_26=451,21
PRIMER_RIGHT_26=1342,20
PRIMER_LEFT_26_TM=61.084
PRIMER_RIGHT_26_TM=57.380
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.3000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=892
PRIMER_PAIR_27_PENALTY=4.750738
PRIMER_LEFT_27_PENALTY=2.130883
PRIMER_RIGHT_27_PENALTY=2.619855
PRIMER_LEFT_27_SEQUENCE=CTTCTACAATTTGCATCCACGA
PRIMER_RIGHT_27_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_27=710,22
PRIMER_RIGHT_27=1342,20
PRIMER_LEFT_27_TM=60.131
PRIMER_RIGHT_27_TM=57.380
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=6.3000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=633
PRIMER_PAIR_28_PENALTY=4.816600
PRIMER_LEFT_28_PENALTY=2.196745
PRIMER_RIGHT_28_PENALTY=2.619855
PRIMER_LEFT_28_SEQUENCE=TCCTTGGTAGGACAGCAGATT
PRIMER_RIGHT_28_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_28=500,21
PRIMER_RIGHT_28=1342,20
PRIMER_LEFT_28_TM=58.803
PRIMER_RIGHT_28_TM=57.380
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=6.3000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=843
PRIMER_PAIR_29_PENALTY=4.822852
PRIMER_LEFT_29_PENALTY=2.202997
PRIMER_RIGHT_29_PENALTY=2.619855
PRIMER_LEFT_29_SEQUENCE=CACAGTTTTGCCTTCCATCT
PRIMER_RIGHT_29_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_29=577,20
PRIMER_RIGHT_29=1342,20
PRIMER_LEFT_29_TM=57.797
PRIMER_RIGHT_29_TM=57.380
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=6.3000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=766
PRIMER_PAIR_30_PENALTY=4.839647
PRIMER_LEFT_30_PENALTY=2.219792
PRIMER_RIGHT_30_PENALTY=2.619855
PRIMER_LEFT_30_SEQUENCE=GAGATCACAGTTTTGCCTTCC
PRIMER_RIGHT_30_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_30=572,21
PRIMER_RIGHT_30=1342,20
PRIMER_LEFT_30_TM=58.780
PRIMER_RIGHT_30_TM=57.380
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.3000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=771
PRIMER_PAIR_31_PENALTY=4.856878
PRIMER_LEFT_31_PENALTY=2.237023
PRIMER_RIGHT_31_PENALTY=2.619855
PRIMER_LEFT_31_SEQUENCE=TTTGTGATGAAGCAACACTCC
PRIMER_RIGHT_31_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_31=675,21
PRIMER_RIGHT_31=1342,20
PRIMER_LEFT_31_TM=58.763
PRIMER_RIGHT_31_TM=57.380
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=6.3000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=668
PRIMER_PAIR_32_PENALTY=4.882653
PRIMER_LEFT_32_PENALTY=2.262798
PRIMER_RIGHT_32_PENALTY=2.619855
PRIMER_LEFT_32_SEQUENCE=AATGGTGTTCGTCAGGGGAG
PRIMER_RIGHT_32_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_32=794,20
PRIMER_RIGHT_32=1342,20
PRIMER_LEFT_32_TM=62.263
PRIMER_RIGHT_32_TM=57.380
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=6.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=549
PRIMER_PAIR_33_PENALTY=4.918191
PRIMER_LEFT_33_PENALTY=2.298336
PRIMER_RIGHT_33_PENALTY=2.619855
PRIMER_LEFT_33_SEQUENCE=TCTACAATTTGCATCCACGA
PRIMER_RIGHT_33_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_33=712,20
PRIMER_RIGHT_33=1342,20
PRIMER_LEFT_33_TM=57.702
PRIMER_RIGHT_33_TM=57.380
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=6.3000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=631
PRIMER_PAIR_34_PENALTY=4.941264
PRIMER_LEFT_34_PENALTY=2.321409
PRIMER_RIGHT_34_PENALTY=2.619855
PRIMER_LEFT_34_SEQUENCE=CCCACTCCCATGTCAGTTTCT
PRIMER_RIGHT_34_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_34=928,21
PRIMER_RIGHT_34=1342,20
PRIMER_LEFT_34_TM=61.321
PRIMER_RIGHT_34_TM=57.380
PRIMER_LEFT_34_GC_PERCENT=52.381
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.3000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=415
PRIMER_PAIR_35_PENALTY=4.966984
PRIMER_LEFT_35_PENALTY=2.347129
PRIMER_RIGHT_35_PENALTY=2.619855
PRIMER_LEFT_35_SEQUENCE=CCTTGGTAGGACAGCAGATTTT
PRIMER_RIGHT_35_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_35=501,22
PRIMER_RIGHT_35=1342,20
PRIMER_LEFT_35_TM=59.653
PRIMER_RIGHT_35_TM=57.380
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.2000
PRIMER_RIGHT_35_END_STABILITY=6.3000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=842
PRIMER_PAIR_36_PENALTY=5.028036
PRIMER_LEFT_36_PENALTY=2.408180
PRIMER_RIGHT_36_PENALTY=2.619855
PRIMER_LEFT_36_SEQUENCE=TATCATCCTTGGTAGGACAGCA
PRIMER_RIGHT_36_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_36=495,22
PRIMER_RIGHT_36=1342,20
PRIMER_LEFT_36_TM=59.592
PRIMER_RIGHT_36_TM=57.380
PRIMER_LEFT_36_GC_PERCENT=45.455
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=6.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=848
PRIMER_PAIR_37_PENALTY=5.072167
PRIMER_LEFT_37_PENALTY=2.452312
PRIMER_RIGHT_37_PENALTY=2.619855
PRIMER_LEFT_37_SEQUENCE=CCACTCCCATGTCAGTTTCT
PRIMER_RIGHT_37_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_37=929,20
PRIMER_RIGHT_37=1342,20
PRIMER_LEFT_37_TM=57.548
PRIMER_RIGHT_37_TM=57.380
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=6.3000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=414
PRIMER_PAIR_38_PENALTY=5.133686
PRIMER_LEFT_38_PENALTY=2.513831
PRIMER_RIGHT_38_PENALTY=2.619855
PRIMER_LEFT_38_SEQUENCE=CACAGTTTTGCCTTCCATCTTT
PRIMER_RIGHT_38_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_38=577,22
PRIMER_RIGHT_38=1342,20
PRIMER_LEFT_38_TM=60.514
PRIMER_RIGHT_38_TM=57.380
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=766
PRIMER_PAIR_39_PENALTY=5.260657
PRIMER_LEFT_39_PENALTY=2.640802
PRIMER_RIGHT_39_PENALTY=2.619855
PRIMER_LEFT_39_SEQUENCE=TTGAGATCACAGTTTTGCCTTC
PRIMER_RIGHT_39_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_39=570,22
PRIMER_RIGHT_39=1342,20
PRIMER_LEFT_39_TM=59.359
PRIMER_RIGHT_39_TM=57.380
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=6.3000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=773
PRIMER_PAIR_40_PENALTY=5.281682
PRIMER_LEFT_40_PENALTY=2.661827
PRIMER_RIGHT_40_PENALTY=2.619855
PRIMER_LEFT_40_SEQUENCE=GTTTTGCCTTCCATCTTTTG
PRIMER_RIGHT_40_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_40=581,20
PRIMER_RIGHT_40=1342,20
PRIMER_LEFT_40_TM=57.338
PRIMER_RIGHT_40_TM=57.380
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=6.3000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=762
PRIMER_PAIR_41_PENALTY=5.297103
PRIMER_LEFT_41_PENALTY=2.677248
PRIMER_RIGHT_41_PENALTY=2.619855
PRIMER_LEFT_41_SEQUENCE=ATCCTTGGTAGGACAGCAGA
PRIMER_RIGHT_41_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_41=499,20
PRIMER_RIGHT_41=1342,20
PRIMER_LEFT_41_TM=57.323
PRIMER_RIGHT_41_TM=57.380
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.3000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=844
PRIMER_PAIR_42_PENALTY=5.306026
PRIMER_LEFT_42_PENALTY=2.686171
PRIMER_RIGHT_42_PENALTY=2.619855
PRIMER_LEFT_42_SEQUENCE=ATATACTGCGCCCACTCCCA
PRIMER_RIGHT_42_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_42=918,20
PRIMER_RIGHT_42=1342,20
PRIMER_LEFT_42_TM=62.686
PRIMER_RIGHT_42_TM=57.380
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=6.3000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=425
PRIMER_PAIR_43_PENALTY=5.306026
PRIMER_LEFT_43_PENALTY=2.686171
PRIMER_RIGHT_43_PENALTY=2.619855
PRIMER_LEFT_43_SEQUENCE=CATATACTGCGCCCACTCCC
PRIMER_RIGHT_43_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_43=917,20
PRIMER_RIGHT_43=1342,20
PRIMER_LEFT_43_TM=62.686
PRIMER_RIGHT_43_TM=57.380
PRIMER_LEFT_43_GC_PERCENT=60.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=426
PRIMER_PAIR_44_PENALTY=5.329685
PRIMER_LEFT_44_PENALTY=2.709830
PRIMER_RIGHT_44_PENALTY=2.619855
PRIMER_LEFT_44_SEQUENCE=TGTGATGAAGCAACACTCCGT
PRIMER_RIGHT_44_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_44=677,21
PRIMER_RIGHT_44=1342,20
PRIMER_LEFT_44_TM=61.710
PRIMER_RIGHT_44_TM=57.380
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=6.3000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=666
PRIMER_PAIR_45_PENALTY=5.342819
PRIMER_LEFT_45_PENALTY=2.722964
PRIMER_RIGHT_45_PENALTY=2.619855
PRIMER_LEFT_45_SEQUENCE=ATAAAATTGGGATGACACGG
PRIMER_RIGHT_45_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_45=448,20
PRIMER_RIGHT_45=1342,20
PRIMER_LEFT_45_TM=57.277
PRIMER_RIGHT_45_TM=57.380
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.9000
PRIMER_RIGHT_45_END_STABILITY=6.3000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=895
PRIMER_PAIR_46_PENALTY=5.392959
PRIMER_LEFT_46_PENALTY=2.773104
PRIMER_RIGHT_46_PENALTY=2.619855
PRIMER_LEFT_46_SEQUENCE=CTACAATTTGCATCCACGAA
PRIMER_RIGHT_46_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_46=713,20
PRIMER_RIGHT_46=1342,20
PRIMER_LEFT_46_TM=57.227
PRIMER_RIGHT_46_TM=57.380
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.4000
PRIMER_RIGHT_46_END_STABILITY=6.3000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=630
PRIMER_PAIR_47_PENALTY=5.417108
PRIMER_LEFT_47_PENALTY=2.797253
PRIMER_RIGHT_47_PENALTY=2.619855
PRIMER_LEFT_47_SEQUENCE=TTGTGATGAAGCAACACTCC
PRIMER_RIGHT_47_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_47=676,20
PRIMER_RIGHT_47=1342,20
PRIMER_LEFT_47_TM=57.203
PRIMER_RIGHT_47_TM=57.380
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=667
PRIMER_PAIR_48_PENALTY=5.481326
PRIMER_LEFT_48_PENALTY=2.861470
PRIMER_RIGHT_48_PENALTY=2.619855
PRIMER_LEFT_48_SEQUENCE=ATGGTGTTCGTCAGGGGAGA
PRIMER_RIGHT_48_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_48=795,20
PRIMER_RIGHT_48=1342,20
PRIMER_LEFT_48_TM=62.861
PRIMER_RIGHT_48_TM=57.380
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=548
PRIMER_PAIR_49_PENALTY=5.485477
PRIMER_LEFT_49_PENALTY=2.865622
PRIMER_RIGHT_49_PENALTY=2.619855
PRIMER_LEFT_49_SEQUENCE=GATCACAGTTTTGCCTTCCATC
PRIMER_RIGHT_49_SEQUENCE=TTCGCAGCAAACATTATCAC
PRIMER_LEFT_49=574,22
PRIMER_RIGHT_49=1342,20
PRIMER_LEFT_49_TM=60.866
PRIMER_RIGHT_49_TM=57.380
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=6.3000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=769
=
SEQUENCE_ID=MAGIC.105_4_10932544_10932749_zu-0_mt-0_33861_229443_TRUE
SEQUENCE_TARGET=950,305
SEQUENCE_TEMPLATE=GGCCACACTCAATGATCATTCTTCAACAGNTGAATTGGACACGCTCTNTNTTTTCTCNTCAATGTGTGTGCGCAATTGATGGAAAGACAATGAGAGGAAACTTACGACAAACATCANTACCAGATGCTTCAGCTAGATCAGGCTTATCATCTCCTNCTGGTCCGACCTTGAGTCTCCAGTTGAANCGTTTCACAAGGTTGGCTAGTGCNAATTTGGACAAAACCAAAGAAAATCCGATTCCAGGGCAAAGTCTTCTCCCTGCTCCAAATGGAACGAANTTGGAATCNGTNCGTTNAAATCCAAATTNGTACNAAAATGNCTCTCCGGTCTATATTCATNNGCATCAAGCCCCCATATCGCAGGGTTTCGTTGGAGTGCCCAAGCATTGATTATNACCTGTGTTCNAAGTCNAGCACATTTTTANATTTAGGTTTACNNTTATAAGAANNTNAAAAAANANNNNAAAAAAANACNTTTACATGTGTCCCNGCAGAAATGTCATATCCCTTTAATTGGACATNTTNACTTGGNAGTCTGAACAATAGTGGACCAGAGGGATGCAACCGTAGCCCCTCCTTAATCACGCAATGTANATAGTTCATTTTCTCAACTTCTTTCTCCGTTACATTCAAATTATGTGTCGAAAATGAATTAATCTCATCTTGAAGTTTTTTCATACACTCTGGATGTCTCATAAGCTCTGTCATTGTCCATTCTAATTGTGAGGCAGTTGTTGCTGTTCCAGAGAAAAACATATCCTGNANAATNAACCAAGGAAAAACAAAATTGATTTAGCTATCGATTGATACTTATCAATTTGGTTTTANTCCAAATTNATAGGACTANTNNNNNNNAAACATTTGTTTNAATACAAGTAANTTNTACACNNNNNNNNNNNNACTTTTTCNATCAGATNTCTCNTATGTTTTTTACAAAACAAAAACGGGGNAANAATTGTTTTTGTTTTACCNAAACATATNNNNANTAACTTATNTAAAAANATTTGACATCATGTACTNCAAATTCATTTTTCTGNAAATATACAAAACGAAAAGGGGTGNCTTGTGTTACCTTGAGNATGAGTATTAAGTCACTTCTGTCAAACTNAAACCGTTTGGTTTTATCCTTTTGAATCAGTAGCAACATATCAATAAAATCTGATGGCTCTTTGTGATCGGCATCNACATGTTCTTGCACCATGCTGTNTAAAAAACAATCTAACTTGTTATTTATGTCCTTCATTTTNTCATCTATGCCACTGNTCCAGTCTATCCATGCCAAACTAGGAATAAAATCTCCAAAAAAGAATTTGCCAAAAAACTCAGAGGATGTCATCACTAAGTTCTTGATATCGATTCCACCTTCCTCTNCGTTATATTTTCTTCCCAAGGTGATTCTGCATATGATATCGTTGGTAAGAGTCATAAGGAGTTTGCTTAGATTTACTGATGAAGAAGATGAAGATGCTTCTTCCAGCTTCTCTGTCATTACTTTGATCTCTTCTTCTCTTAGGTTCTCAAAGGACCTAACCATTTTGTTNTTGAGCAGATGTACCACNCCCAGACTCTGCAAGCATCNCCAGTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.227709
PRIMER_LEFT_0_PENALTY=0.032351
PRIMER_RIGHT_0_PENALTY=0.195358
PRIMER_LEFT_0_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_0_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_0=722,20
PRIMER_RIGHT_0=1399,20
PRIMER_LEFT_0_TM=60.032
PRIMER_RIGHT_0_TM=60.195
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=678
PRIMER_PAIR_1_PENALTY=0.493217
PRIMER_LEFT_1_PENALTY=0.032351
PRIMER_RIGHT_1_PENALTY=0.460865
PRIMER_LEFT_1_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_1_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_1=722,20
PRIMER_RIGHT_1=1401,20
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.461
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=680
PRIMER_PAIR_2_PENALTY=0.705470
PRIMER_LEFT_2_PENALTY=0.510112
PRIMER_RIGHT_2_PENALTY=0.195358
PRIMER_LEFT_2_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_2_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_2=720,20
PRIMER_RIGHT_2=1399,20
PRIMER_LEFT_2_TM=60.510
PRIMER_RIGHT_2_TM=60.195
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=680
PRIMER_PAIR_3_PENALTY=0.753105
PRIMER_LEFT_3_PENALTY=0.557747
PRIMER_RIGHT_3_PENALTY=0.195358
PRIMER_LEFT_3_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_3_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_3=562,20
PRIMER_RIGHT_3=1399,20
PRIMER_LEFT_3_TM=59.442
PRIMER_RIGHT_3_TM=60.195
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=838
PRIMER_PAIR_4_PENALTY=0.957107
PRIMER_LEFT_4_PENALTY=0.032351
PRIMER_RIGHT_4_PENALTY=0.924755
PRIMER_LEFT_4_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_4_SEQUENCE=TTTGGCATGGATAGACTGGA
PRIMER_LEFT_4=722,20
PRIMER_RIGHT_4=1281,20
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=59.075
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=560
PRIMER_PAIR_5_PENALTY=0.970977
PRIMER_LEFT_5_PENALTY=0.510112
PRIMER_RIGHT_5_PENALTY=0.460865
PRIMER_LEFT_5_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_5_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_5=720,20
PRIMER_RIGHT_5=1401,20
PRIMER_LEFT_5_TM=60.510
PRIMER_RIGHT_5_TM=60.461
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=682
PRIMER_PAIR_6_PENALTY=1.018613
PRIMER_LEFT_6_PENALTY=0.557747
PRIMER_RIGHT_6_PENALTY=0.460865
PRIMER_LEFT_6_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_6_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_6=562,20
PRIMER_RIGHT_6=1401,20
PRIMER_LEFT_6_TM=59.442
PRIMER_RIGHT_6_TM=60.461
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.9000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=840
PRIMER_PAIR_7_PENALTY=1.085048
PRIMER_LEFT_7_PENALTY=0.032351
PRIMER_RIGHT_7_PENALTY=1.052696
PRIMER_LEFT_7_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_7_SEQUENCE=GTTTGGCATGGATAGACTGGA
PRIMER_LEFT_7=722,20
PRIMER_RIGHT_7=1282,21
PRIMER_LEFT_7_TM=60.032
PRIMER_RIGHT_7_TM=59.947
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=561
PRIMER_PAIR_8_PENALTY=1.249258
PRIMER_LEFT_8_PENALTY=1.053900
PRIMER_RIGHT_8_PENALTY=0.195358
PRIMER_LEFT_8_SEQUENCE=TTGTGAGGCAGTTGTTGCTG
PRIMER_RIGHT_8_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_8=719,20
PRIMER_RIGHT_8=1399,20
PRIMER_LEFT_8_TM=61.054
PRIMER_RIGHT_8_TM=60.195
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=681
PRIMER_PAIR_9_PENALTY=1.259941
PRIMER_LEFT_9_PENALTY=1.064583
PRIMER_RIGHT_9_PENALTY=0.195358
PRIMER_LEFT_9_SEQUENCE=GTGAGGCAGTTGTTGCTGTT
PRIMER_RIGHT_9_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_9=721,20
PRIMER_RIGHT_9=1399,20
PRIMER_LEFT_9_TM=58.935
PRIMER_RIGHT_9_TM=60.195
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=679
PRIMER_PAIR_10_PENALTY=1.369780
PRIMER_LEFT_10_PENALTY=0.032351
PRIMER_RIGHT_10_PENALTY=1.337428
PRIMER_LEFT_10_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_10_SEQUENCE=CATATGCAGAATCACCTTGGG
PRIMER_LEFT_10=722,20
PRIMER_RIGHT_10=1404,21
PRIMER_LEFT_10_TM=60.032
PRIMER_RIGHT_10_TM=60.337
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=10.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=683
PRIMER_PAIR_11_PENALTY=1.400764
PRIMER_LEFT_11_PENALTY=1.205406
PRIMER_RIGHT_11_PENALTY=0.195358
PRIMER_LEFT_11_SEQUENCE=AATTGTGAGGCAGTTGTTGCT
PRIMER_RIGHT_11_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_11=717,21
PRIMER_RIGHT_11=1399,20
PRIMER_LEFT_11_TM=59.795
PRIMER_RIGHT_11_TM=60.195
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=683
PRIMER_PAIR_12_PENALTY=1.434867
PRIMER_LEFT_12_PENALTY=0.510112
PRIMER_RIGHT_12_PENALTY=0.924755
PRIMER_LEFT_12_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_12_SEQUENCE=TTTGGCATGGATAGACTGGA
PRIMER_LEFT_12=720,20
PRIMER_RIGHT_12=1281,20
PRIMER_LEFT_12_TM=60.510
PRIMER_RIGHT_12_TM=59.075
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=562
PRIMER_PAIR_13_PENALTY=1.482502
PRIMER_LEFT_13_PENALTY=0.557747
PRIMER_RIGHT_13_PENALTY=0.924755
PRIMER_LEFT_13_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_13_SEQUENCE=TTTGGCATGGATAGACTGGA
PRIMER_LEFT_13=562,20
PRIMER_RIGHT_13=1281,20
PRIMER_LEFT_13_TM=59.442
PRIMER_RIGHT_13_TM=59.075
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=5.9000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=720
PRIMER_PAIR_14_PENALTY=1.486383
PRIMER_LEFT_14_PENALTY=0.032351
PRIMER_RIGHT_14_PENALTY=1.454032
PRIMER_LEFT_14_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_14_SEQUENCE=TATGCAGAATCACCTTGGGAA
PRIMER_LEFT_14=722,20
PRIMER_RIGHT_14=1402,21
PRIMER_LEFT_14_TM=60.032
PRIMER_RIGHT_14_TM=60.454
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=681
PRIMER_PAIR_15_PENALTY=1.514766
PRIMER_LEFT_15_PENALTY=1.053900
PRIMER_RIGHT_15_PENALTY=0.460865
PRIMER_LEFT_15_SEQUENCE=TTGTGAGGCAGTTGTTGCTG
PRIMER_RIGHT_15_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_15=719,20
PRIMER_RIGHT_15=1401,20
PRIMER_LEFT_15_TM=61.054
PRIMER_RIGHT_15_TM=60.461
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=683
PRIMER_PAIR_16_PENALTY=1.524776
PRIMER_LEFT_16_PENALTY=1.329419
PRIMER_RIGHT_16_PENALTY=0.195358
PRIMER_LEFT_16_SEQUENCE=CGTAGCCCCTCCTTAATCAC
PRIMER_RIGHT_16_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_16=564,20
PRIMER_RIGHT_16=1399,20
PRIMER_LEFT_16_TM=58.671
PRIMER_RIGHT_16_TM=60.195
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.3000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=836
PRIMER_PAIR_17_PENALTY=1.525449
PRIMER_LEFT_17_PENALTY=1.064583
PRIMER_RIGHT_17_PENALTY=0.460865
PRIMER_LEFT_17_SEQUENCE=GTGAGGCAGTTGTTGCTGTT
PRIMER_RIGHT_17_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_17=721,20
PRIMER_RIGHT_17=1401,20
PRIMER_LEFT_17_TM=58.935
PRIMER_RIGHT_17_TM=60.461
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=681
PRIMER_PAIR_18_PENALTY=1.562808
PRIMER_LEFT_18_PENALTY=0.510112
PRIMER_RIGHT_18_PENALTY=1.052696
PRIMER_LEFT_18_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_18_SEQUENCE=GTTTGGCATGGATAGACTGGA
PRIMER_LEFT_18=720,20
PRIMER_RIGHT_18=1282,21
PRIMER_LEFT_18_TM=60.510
PRIMER_RIGHT_18_TM=59.947
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=563
PRIMER_PAIR_19_PENALTY=1.610443
PRIMER_LEFT_19_PENALTY=0.557747
PRIMER_RIGHT_19_PENALTY=1.052696
PRIMER_LEFT_19_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_19_SEQUENCE=GTTTGGCATGGATAGACTGGA
PRIMER_LEFT_19=562,20
PRIMER_RIGHT_19=1282,21
PRIMER_LEFT_19_TM=59.442
PRIMER_RIGHT_19_TM=59.947
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=721
PRIMER_PAIR_20_PENALTY=1.666271
PRIMER_LEFT_20_PENALTY=1.205406
PRIMER_RIGHT_20_PENALTY=0.460865
PRIMER_LEFT_20_SEQUENCE=AATTGTGAGGCAGTTGTTGCT
PRIMER_RIGHT_20_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_20=717,21
PRIMER_RIGHT_20=1401,20
PRIMER_LEFT_20_TM=59.795
PRIMER_RIGHT_20_TM=60.461
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=685
PRIMER_PAIR_21_PENALTY=1.768469
PRIMER_LEFT_21_PENALTY=0.843714
PRIMER_RIGHT_21_PENALTY=0.924755
PRIMER_LEFT_21_SEQUENCE=GAGGCAGTTGTTGCTGTTCC
PRIMER_RIGHT_21_SEQUENCE=TTTGGCATGGATAGACTGGA
PRIMER_LEFT_21=723,20
PRIMER_RIGHT_21=1281,20
PRIMER_LEFT_21_TM=60.844
PRIMER_RIGHT_21_TM=59.075
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=559
PRIMER_PAIR_22_PENALTY=1.780361
PRIMER_LEFT_22_PENALTY=1.585003
PRIMER_RIGHT_22_PENALTY=0.195358
PRIMER_LEFT_22_SEQUENCE=CAATAGTGGACCAGAGGGATG
PRIMER_RIGHT_22_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_22=539,21
PRIMER_RIGHT_22=1399,20
PRIMER_LEFT_22_TM=59.415
PRIMER_RIGHT_22_TM=60.195
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=861
PRIMER_PAIR_23_PENALTY=1.790284
PRIMER_LEFT_23_PENALTY=1.329419
PRIMER_RIGHT_23_PENALTY=0.460865
PRIMER_LEFT_23_SEQUENCE=CGTAGCCCCTCCTTAATCAC
PRIMER_RIGHT_23_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_23=564,20
PRIMER_RIGHT_23=1401,20
PRIMER_LEFT_23_TM=58.671
PRIMER_RIGHT_23_TM=60.461
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.3000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=838
PRIMER_PAIR_24_PENALTY=1.824315
PRIMER_LEFT_24_PENALTY=1.628957
PRIMER_RIGHT_24_PENALTY=0.195358
PRIMER_LEFT_24_SEQUENCE=ATTGTGAGGCAGTTGTTGCT
PRIMER_RIGHT_24_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_24=718,20
PRIMER_RIGHT_24=1399,20
PRIMER_LEFT_24_TM=58.371
PRIMER_RIGHT_24_TM=60.195
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=682
PRIMER_PAIR_25_PENALTY=1.847540
PRIMER_LEFT_25_PENALTY=0.510112
PRIMER_RIGHT_25_PENALTY=1.337428
PRIMER_LEFT_25_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_25_SEQUENCE=CATATGCAGAATCACCTTGGG
PRIMER_LEFT_25=720,20
PRIMER_RIGHT_25=1404,21
PRIMER_LEFT_25_TM=60.510
PRIMER_RIGHT_25_TM=60.337
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=10.0000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=685
PRIMER_PAIR_26_PENALTY=1.865604
PRIMER_LEFT_26_PENALTY=1.670246
PRIMER_RIGHT_26_PENALTY=0.195358
PRIMER_LEFT_26_SEQUENCE=AACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_26_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_26=561,21
PRIMER_RIGHT_26=1399,20
PRIMER_LEFT_26_TM=60.670
PRIMER_RIGHT_26_TM=60.195
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=5.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=839
PRIMER_PAIR_27_PENALTY=1.875850
PRIMER_LEFT_27_PENALTY=0.032351
PRIMER_RIGHT_27_PENALTY=1.843499
PRIMER_LEFT_27_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_27_SEQUENCE=CAGAATCACCTTGGGAAGAAA
PRIMER_LEFT_27=722,20
PRIMER_RIGHT_27=1398,21
PRIMER_LEFT_27_TM=60.032
PRIMER_RIGHT_27_TM=59.157
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=677
PRIMER_PAIR_28_PENALTY=1.895175
PRIMER_LEFT_28_PENALTY=0.557747
PRIMER_RIGHT_28_PENALTY=1.337428
PRIMER_LEFT_28_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_28_SEQUENCE=CATATGCAGAATCACCTTGGG
PRIMER_LEFT_28=562,20
PRIMER_RIGHT_28=1404,21
PRIMER_LEFT_28_TM=59.442
PRIMER_RIGHT_28_TM=60.337
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=5.9000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=843
PRIMER_PAIR_29_PENALTY=1.896410
PRIMER_LEFT_29_PENALTY=0.843714
PRIMER_RIGHT_29_PENALTY=1.052696
PRIMER_LEFT_29_SEQUENCE=GAGGCAGTTGTTGCTGTTCC
PRIMER_RIGHT_29_SEQUENCE=GTTTGGCATGGATAGACTGGA
PRIMER_LEFT_29=723,20
PRIMER_RIGHT_29=1282,21
PRIMER_LEFT_29_TM=60.844
PRIMER_RIGHT_29_TM=59.947
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=560
PRIMER_PAIR_30_PENALTY=1.964144
PRIMER_LEFT_30_PENALTY=0.510112
PRIMER_RIGHT_30_PENALTY=1.454032
PRIMER_LEFT_30_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_30_SEQUENCE=TATGCAGAATCACCTTGGGAA
PRIMER_LEFT_30=720,20
PRIMER_RIGHT_30=1402,21
PRIMER_LEFT_30_TM=60.510
PRIMER_RIGHT_30_TM=60.454
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=683
PRIMER_PAIR_31_PENALTY=1.966228
PRIMER_LEFT_31_PENALTY=0.032351
PRIMER_RIGHT_31_PENALTY=1.933877
PRIMER_LEFT_31_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_31_SEQUENCE=AGTTTGGCATGGATAGACTGG
PRIMER_LEFT_31=722,20
PRIMER_RIGHT_31=1283,21
PRIMER_LEFT_31_TM=60.032
PRIMER_RIGHT_31_TM=59.066
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=562
PRIMER_PAIR_32_PENALTY=1.978656
PRIMER_LEFT_32_PENALTY=1.053900
PRIMER_RIGHT_32_PENALTY=0.924755
PRIMER_LEFT_32_SEQUENCE=TTGTGAGGCAGTTGTTGCTG
PRIMER_RIGHT_32_SEQUENCE=TTTGGCATGGATAGACTGGA
PRIMER_LEFT_32=719,20
PRIMER_RIGHT_32=1281,20
PRIMER_LEFT_32_TM=61.054
PRIMER_RIGHT_32_TM=59.075
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=563
PRIMER_PAIR_33_PENALTY=1.989338
PRIMER_LEFT_33_PENALTY=1.064583
PRIMER_RIGHT_33_PENALTY=0.924755
PRIMER_LEFT_33_SEQUENCE=GTGAGGCAGTTGTTGCTGTT
PRIMER_RIGHT_33_SEQUENCE=TTTGGCATGGATAGACTGGA
PRIMER_LEFT_33=721,20
PRIMER_RIGHT_33=1281,20
PRIMER_LEFT_33_TM=58.935
PRIMER_RIGHT_33_TM=59.075
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=561
PRIMER_PAIR_34_PENALTY=2.011779
PRIMER_LEFT_34_PENALTY=0.557747
PRIMER_RIGHT_34_PENALTY=1.454032
PRIMER_LEFT_34_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_34_SEQUENCE=TATGCAGAATCACCTTGGGAA
PRIMER_LEFT_34=562,20
PRIMER_RIGHT_34=1402,21
PRIMER_LEFT_34_TM=59.442
PRIMER_RIGHT_34_TM=60.454
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=5.9000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=841
PRIMER_PAIR_35_PENALTY=2.013089
PRIMER_LEFT_35_PENALTY=0.032351
PRIMER_RIGHT_35_PENALTY=1.980738
PRIMER_LEFT_35_SEQUENCE=TGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_35_SEQUENCE=GTTTGGCATGGATAGACTGG
PRIMER_LEFT_35=722,20
PRIMER_RIGHT_35=1282,20
PRIMER_LEFT_35_TM=60.032
PRIMER_RIGHT_35_TM=58.019
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=561
PRIMER_PAIR_36_PENALTY=2.028293
PRIMER_LEFT_36_PENALTY=1.832935
PRIMER_RIGHT_36_PENALTY=0.195358
PRIMER_LEFT_36_SEQUENCE=AGGCAGTTGTTGCTGTTCCA
PRIMER_RIGHT_36_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_36=724,20
PRIMER_RIGHT_36=1399,20
PRIMER_LEFT_36_TM=61.833
PRIMER_RIGHT_36_TM=60.195
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=676
PRIMER_PAIR_37_PENALTY=2.040748
PRIMER_LEFT_37_PENALTY=0.510112
PRIMER_RIGHT_37_PENALTY=1.530636
PRIMER_LEFT_37_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_37_SEQUENCE=CAGAGAAGCTGGAAGAAGCA
PRIMER_LEFT_37=720,20
PRIMER_RIGHT_37=1483,20
PRIMER_LEFT_37_TM=60.510
PRIMER_RIGHT_37_TM=58.469
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=764
PRIMER_PAIR_38_PENALTY=2.045868
PRIMER_LEFT_38_PENALTY=1.585003
PRIMER_RIGHT_38_PENALTY=0.460865
PRIMER_LEFT_38_SEQUENCE=CAATAGTGGACCAGAGGGATG
PRIMER_RIGHT_38_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_38=539,21
PRIMER_RIGHT_38=1401,20
PRIMER_LEFT_38_TM=59.415
PRIMER_RIGHT_38_TM=60.461
PRIMER_LEFT_38_GC_PERCENT=52.381
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=863
PRIMER_PAIR_39_PENALTY=2.086158
PRIMER_LEFT_39_PENALTY=1.890800
PRIMER_RIGHT_39_PENALTY=0.195358
PRIMER_LEFT_39_SEQUENCE=GTGAGGCAGTTGTTGCTGTTC
PRIMER_RIGHT_39_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_39=721,21
PRIMER_RIGHT_39=1399,20
PRIMER_LEFT_39_TM=60.891
PRIMER_RIGHT_39_TM=60.195
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=679
PRIMER_PAIR_40_PENALTY=2.088384
PRIMER_LEFT_40_PENALTY=0.557747
PRIMER_RIGHT_40_PENALTY=1.530636
PRIMER_LEFT_40_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_40_SEQUENCE=CAGAGAAGCTGGAAGAAGCA
PRIMER_LEFT_40=562,20
PRIMER_RIGHT_40=1483,20
PRIMER_LEFT_40_TM=59.442
PRIMER_RIGHT_40_TM=58.469
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.9000
PRIMER_RIGHT_40_END_STABILITY=8.5000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=922
PRIMER_PAIR_41_PENALTY=2.089822
PRIMER_LEFT_41_PENALTY=1.628957
PRIMER_RIGHT_41_PENALTY=0.460865
PRIMER_LEFT_41_SEQUENCE=ATTGTGAGGCAGTTGTTGCT
PRIMER_RIGHT_41_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_41=718,20
PRIMER_RIGHT_41=1401,20
PRIMER_LEFT_41_TM=58.371
PRIMER_RIGHT_41_TM=60.461
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=684
PRIMER_PAIR_42_PENALTY=2.106596
PRIMER_LEFT_42_PENALTY=1.053900
PRIMER_RIGHT_42_PENALTY=1.052696
PRIMER_LEFT_42_SEQUENCE=TTGTGAGGCAGTTGTTGCTG
PRIMER_RIGHT_42_SEQUENCE=GTTTGGCATGGATAGACTGGA
PRIMER_LEFT_42=719,20
PRIMER_RIGHT_42=1282,21
PRIMER_LEFT_42_TM=61.054
PRIMER_RIGHT_42_TM=59.947
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=564
PRIMER_PAIR_43_PENALTY=2.117279
PRIMER_LEFT_43_PENALTY=1.064583
PRIMER_RIGHT_43_PENALTY=1.052696
PRIMER_LEFT_43_SEQUENCE=GTGAGGCAGTTGTTGCTGTT
PRIMER_RIGHT_43_SEQUENCE=GTTTGGCATGGATAGACTGGA
PRIMER_LEFT_43=721,20
PRIMER_RIGHT_43=1282,21
PRIMER_LEFT_43_TM=58.935
PRIMER_RIGHT_43_TM=59.947
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=562
PRIMER_PAIR_44_PENALTY=2.124121
PRIMER_LEFT_44_PENALTY=1.928763
PRIMER_RIGHT_44_PENALTY=0.195358
PRIMER_LEFT_44_SEQUENCE=CAGTTGTTGCTGTTCCAGAGA
PRIMER_RIGHT_44_SEQUENCE=GCAGAATCACCTTGGGAAGA
PRIMER_LEFT_44=727,21
PRIMER_RIGHT_44=1399,20
PRIMER_LEFT_44_TM=59.071
PRIMER_RIGHT_44_TM=60.195
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=673
PRIMER_PAIR_45_PENALTY=2.130161
PRIMER_LEFT_45_PENALTY=1.205406
PRIMER_RIGHT_45_PENALTY=0.924755
PRIMER_LEFT_45_SEQUENCE=AATTGTGAGGCAGTTGTTGCT
PRIMER_RIGHT_45_SEQUENCE=TTTGGCATGGATAGACTGGA
PRIMER_LEFT_45=717,21
PRIMER_RIGHT_45=1281,20
PRIMER_LEFT_45_TM=59.795
PRIMER_RIGHT_45_TM=59.075
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=565
PRIMER_PAIR_46_PENALTY=2.131112
PRIMER_LEFT_46_PENALTY=1.670246
PRIMER_RIGHT_46_PENALTY=0.460865
PRIMER_LEFT_46_SEQUENCE=AACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_46_SEQUENCE=ATGCAGAATCACCTTGGGAA
PRIMER_LEFT_46=561,21
PRIMER_RIGHT_46=1401,20
PRIMER_LEFT_46_TM=60.670
PRIMER_RIGHT_46_TM=60.461
PRIMER_LEFT_46_GC_PERCENT=52.381
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=5.9000
PRIMER_RIGHT_46_END_STABILITY=9.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=841
PRIMER_PAIR_47_PENALTY=2.143117
PRIMER_LEFT_47_PENALTY=0.510112
PRIMER_RIGHT_47_PENALTY=1.633005
PRIMER_LEFT_47_SEQUENCE=TGTGAGGCAGTTGTTGCTGT
PRIMER_RIGHT_47_SEQUENCE=ACAGAGAAGCTGGAAGAAGCA
PRIMER_LEFT_47=720,20
PRIMER_RIGHT_47=1484,21
PRIMER_LEFT_47_TM=60.510
PRIMER_RIGHT_47_TM=59.367
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=765
PRIMER_PAIR_48_PENALTY=2.181142
PRIMER_LEFT_48_PENALTY=0.843714
PRIMER_RIGHT_48_PENALTY=1.337428
PRIMER_LEFT_48_SEQUENCE=GAGGCAGTTGTTGCTGTTCC
PRIMER_RIGHT_48_SEQUENCE=CATATGCAGAATCACCTTGGG
PRIMER_LEFT_48=723,20
PRIMER_RIGHT_48=1404,21
PRIMER_LEFT_48_TM=60.844
PRIMER_RIGHT_48_TM=60.337
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=10.0000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=682
PRIMER_PAIR_49_PENALTY=2.190752
PRIMER_LEFT_49_PENALTY=0.557747
PRIMER_RIGHT_49_PENALTY=1.633005
PRIMER_LEFT_49_SEQUENCE=ACCGTAGCCCCTCCTTAATC
PRIMER_RIGHT_49_SEQUENCE=ACAGAGAAGCTGGAAGAAGCA
PRIMER_LEFT_49=562,20
PRIMER_RIGHT_49=1484,21
PRIMER_LEFT_49_TM=59.442
PRIMER_RIGHT_49_TM=59.367
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.9000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.287_4_875644_875681_mt-0_oy-0_75615_2648_TRUE
SEQUENCE_TARGET=950,137
SEQUENCE_TEMPLATE=CTGACATTTTACGTTGTGNNNTAACTTGTGTATATCAACATTCTAGCTTTGATTACGAAAGAAAACANAAAATTTCTTTAACTAANATCTAATCCANTTTTNATGAATANCNAAGGGTATTATAAGAAATGTTGNACATGTGAGGTTTTACATTACGGTGTGAAAAAGAATAAAACGGTTGACATCTNTAACGTTCAAAGGGGTAAAAATTAATCGAATTTGTGGAANATNAAAAAANATTAGAAGGTCATACAAGAAANGTNNNACATGTNNNNNAGNAATAGAAATCTAGTTATTTTTTTTGTAAANCNGAATATGACNTNTCTNANGATTGATCTNCTGTCNGTACTNNNAAGGGAACCNTNGTTGTATAACAACTGGTNTGTGACANCTNANAGATGGGAGGTAAATCTTACGTTTCACCNGTTAACATCTATTGAATTATGNGTTCAGATGCGTGNAATTCCTCTTTTGTATGTGTGTGAGGAAACTGCTTTANAGATAGCACATGAGTTAGGAAAGATTNTCANTCNNGATTTTCATGANTCCACCACNACTCAAATAGCTTATATCCGTGTGAGAATCCGGTTTNGAATTACAGATCGCCTGAGGTTTTTCCAAAGAATCATTTTTGATTTTGGTGAGGCTGCTNTGATNAGCTTTCAGTATGAAAGNCTTAGNAGAATCTGTAGTAGCTNCTTNNGAATNACTCANCACAGGAANNCATGNCCTTACNGTCAGATTGAGNCTNTCNATAGGGTNACTAACTCNACTGCTCANAGGAATGTNAGAGAAGANGTGTTNATGAGAGATGAAAATCTGAGATCTTCNANGAATTCNCANTCTCAGATGTNTGAGAGNTCGTTTCNNNNNNNNNNCGATCCACCTCCNAGGNTTCCTCATCCTCCTTTAAANCCTGATGAATTGGTGGCTGCATATTACCCTCNTACAAGAGCTACTTCTTTACCAAACNTTGCAGGTCCNTTGCCTNAGGTACCTCNTAGAANAAATGTGGNTGAAAGGGANTCTAACATCNANCCTTTCTCNGGACCTGCATTNGNANNANACTCTCCACGNCTNGTGNAAGTAGGTGAATCATCNAGGCAAGGTGAGAATACTCAGAATGTTNACACGGTGGAAAAGGGGGATTCATCAAAAAGAAAGAACATGGGTGGTCNCAGGTTTNAAGNTGATGCANGAAAGNCCAATGAAGATGAGCATATGAATGGAGGGATTCTGAAACCTCCAAAAAAGCGTTGATCACTCTGCNNNAATTCTGTCATNNTTTTTNATTTAGTTGCTTTNAGTTCAATNAAGCAGCCACAAGATNTGAAGAAGATNGTATGGCCTAGTATCCCTTTTTGGTTTTGGCANACCNGAAGNGTAATGTNATGTGGTNTAATGCTCTACTCTAANNNNNNNNNNNNNNNTCTTTTGTTTTACAACTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTAAGTGGTAAAAGTGTNTGTCAAACTNTNAAAGTGGATGTTCTTCCACTGTTGNATCGTTTACAACTGCNGTTTTTAATNAAAAGGGTAATACTTATNAGNNNAAAAANNNNNNTNNCTAACGATCAAAAGANTGGANNNNNGGTTCAGTTTGTTTTCACAGTAAATGCAATTTAANCATCAANNNTTTTCACTAATTTTTNACTCTTCCTCANANAAGGNNNNNNNNTAGGATGATAAGTAAACAAATTTAGGAATATNTGTTNTCAANCTTGTATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCAAGCTTGTNTATATTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.691349
PRIMER_LEFT_0_PENALTY=0.186600
PRIMER_RIGHT_0_PENALTY=0.504749
PRIMER_LEFT_0_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_0_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_0=598,20
PRIMER_RIGHT_0=1153,20
PRIMER_LEFT_0_TM=59.813
PRIMER_RIGHT_0_TM=60.505
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=556
PRIMER_PAIR_1_PENALTY=1.083117
PRIMER_LEFT_1_PENALTY=0.578368
PRIMER_RIGHT_1_PENALTY=0.504749
PRIMER_LEFT_1_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_1_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_1=599,20
PRIMER_RIGHT_1=1153,20
PRIMER_LEFT_1_TM=60.578
PRIMER_RIGHT_1_TM=60.505
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=555
PRIMER_PAIR_2_PENALTY=1.181610
PRIMER_LEFT_2_PENALTY=0.186600
PRIMER_RIGHT_2_PENALTY=0.995010
PRIMER_LEFT_2_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_2_SEQUENCE=TTGATGAATCCCCCTTTTCC
PRIMER_LEFT_2=598,20
PRIMER_RIGHT_2=1155,20
PRIMER_LEFT_2_TM=59.813
PRIMER_RIGHT_2_TM=60.995
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=558
PRIMER_PAIR_3_PENALTY=1.280467
PRIMER_LEFT_3_PENALTY=0.775718
PRIMER_RIGHT_3_PENALTY=0.504749
PRIMER_LEFT_3_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_3_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_3=631,20
PRIMER_RIGHT_3=1153,20
PRIMER_LEFT_3_TM=60.776
PRIMER_RIGHT_3_TM=60.505
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=523
PRIMER_PAIR_4_PENALTY=1.379032
PRIMER_LEFT_4_PENALTY=0.186600
PRIMER_RIGHT_4_PENALTY=1.192432
PRIMER_LEFT_4_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_4_SEQUENCE=TTTGATGAATCCCCCTTTTC
PRIMER_LEFT_4=598,20
PRIMER_RIGHT_4=1156,20
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=58.808
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=559
PRIMER_PAIR_5_PENALTY=1.379032
PRIMER_LEFT_5_PENALTY=0.186600
PRIMER_RIGHT_5_PENALTY=1.192432
PRIMER_LEFT_5_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_5_SEQUENCE=TTCTTTTTGATGAATCCCCC
PRIMER_LEFT_5=598,20
PRIMER_RIGHT_5=1161,20
PRIMER_LEFT_5_TM=59.813
PRIMER_RIGHT_5_TM=58.808
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.3000
PRIMER_RIGHT_5_END_STABILITY=12.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=564
PRIMER_PAIR_6_PENALTY=1.573378
PRIMER_LEFT_6_PENALTY=0.578368
PRIMER_RIGHT_6_PENALTY=0.995010
PRIMER_LEFT_6_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_6_SEQUENCE=TTGATGAATCCCCCTTTTCC
PRIMER_LEFT_6=599,20
PRIMER_RIGHT_6=1155,20
PRIMER_LEFT_6_TM=60.578
PRIMER_RIGHT_6_TM=60.995
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=557
PRIMER_PAIR_7_PENALTY=1.673829
PRIMER_LEFT_7_PENALTY=0.186600
PRIMER_RIGHT_7_PENALTY=1.487228
PRIMER_LEFT_7_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_7_SEQUENCE=GCAGAGTGATCAACGCTTTTT
PRIMER_LEFT_7=598,20
PRIMER_RIGHT_7=1268,21
PRIMER_LEFT_7_TM=59.813
PRIMER_RIGHT_7_TM=59.513
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=671
PRIMER_PAIR_8_PENALTY=1.712980
PRIMER_LEFT_8_PENALTY=1.208232
PRIMER_RIGHT_8_PENALTY=0.504749
PRIMER_LEFT_8_SEQUENCE=ACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_8_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_8=597,20
PRIMER_RIGHT_8=1153,20
PRIMER_LEFT_8_TM=58.792
PRIMER_RIGHT_8_TM=60.505
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=557
PRIMER_PAIR_9_PENALTY=1.759671
PRIMER_LEFT_9_PENALTY=1.254922
PRIMER_RIGHT_9_PENALTY=0.504749
PRIMER_LEFT_9_SEQUENCE=TTTTGATTTTGGTGAGGCTG
PRIMER_RIGHT_9_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_9=629,20
PRIMER_RIGHT_9=1153,20
PRIMER_LEFT_9_TM=58.745
PRIMER_RIGHT_9_TM=60.505
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=525
PRIMER_PAIR_10_PENALTY=1.760055
PRIMER_LEFT_10_PENALTY=0.186600
PRIMER_RIGHT_10_PENALTY=1.573455
PRIMER_LEFT_10_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_10_SEQUENCE=TCTTTTTGATGAATCCCCCT
PRIMER_LEFT_10=598,20
PRIMER_RIGHT_10=1160,20
PRIMER_LEFT_10_TM=59.813
PRIMER_RIGHT_10_TM=58.427
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=10.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=563
PRIMER_PAIR_11_PENALTY=1.770728
PRIMER_LEFT_11_PENALTY=0.775718
PRIMER_RIGHT_11_PENALTY=0.995010
PRIMER_LEFT_11_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_11_SEQUENCE=TTGATGAATCCCCCTTTTCC
PRIMER_LEFT_11=631,20
PRIMER_RIGHT_11=1155,20
PRIMER_LEFT_11_TM=60.776
PRIMER_RIGHT_11_TM=60.995
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=525
PRIMER_PAIR_12_PENALTY=1.770800
PRIMER_LEFT_12_PENALTY=0.578368
PRIMER_RIGHT_12_PENALTY=1.192432
PRIMER_LEFT_12_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_12_SEQUENCE=TTTGATGAATCCCCCTTTTC
PRIMER_LEFT_12=599,20
PRIMER_RIGHT_12=1156,20
PRIMER_LEFT_12_TM=60.578
PRIMER_RIGHT_12_TM=58.808
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=558
PRIMER_PAIR_13_PENALTY=1.770800
PRIMER_LEFT_13_PENALTY=0.578368
PRIMER_RIGHT_13_PENALTY=1.192432
PRIMER_LEFT_13_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_13_SEQUENCE=TTCTTTTTGATGAATCCCCC
PRIMER_LEFT_13=599,20
PRIMER_RIGHT_13=1161,20
PRIMER_LEFT_13_TM=60.578
PRIMER_RIGHT_13_TM=58.808
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=12.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=563
PRIMER_PAIR_14_PENALTY=1.968150
PRIMER_LEFT_14_PENALTY=0.775718
PRIMER_RIGHT_14_PENALTY=1.192432
PRIMER_LEFT_14_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_14_SEQUENCE=TTTGATGAATCCCCCTTTTC
PRIMER_LEFT_14=631,20
PRIMER_RIGHT_14=1156,20
PRIMER_LEFT_14_TM=60.776
PRIMER_RIGHT_14_TM=58.808
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=526
PRIMER_PAIR_15_PENALTY=1.968150
PRIMER_LEFT_15_PENALTY=0.775718
PRIMER_RIGHT_15_PENALTY=1.192432
PRIMER_LEFT_15_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_15_SEQUENCE=TTCTTTTTGATGAATCCCCC
PRIMER_LEFT_15=631,20
PRIMER_RIGHT_15=1161,20
PRIMER_LEFT_15_TM=60.776
PRIMER_RIGHT_15_TM=58.808
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=12.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=531
PRIMER_PAIR_16_PENALTY=2.065597
PRIMER_LEFT_16_PENALTY=0.578368
PRIMER_RIGHT_16_PENALTY=1.487228
PRIMER_LEFT_16_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_16_SEQUENCE=GCAGAGTGATCAACGCTTTTT
PRIMER_LEFT_16=599,20
PRIMER_RIGHT_16=1268,21
PRIMER_LEFT_16_TM=60.578
PRIMER_RIGHT_16_TM=59.513
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=670
PRIMER_PAIR_17_PENALTY=2.126243
PRIMER_LEFT_17_PENALTY=0.186600
PRIMER_RIGHT_17_PENALTY=1.939643
PRIMER_LEFT_17_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_17_SEQUENCE=TTTCAGAATCCCTCCATTCA
PRIMER_LEFT_17=598,20
PRIMER_RIGHT_17=1241,20
PRIMER_LEFT_17_TM=59.813
PRIMER_RIGHT_17_TM=58.060
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=644
PRIMER_PAIR_18_PENALTY=2.129263
PRIMER_LEFT_18_PENALTY=1.624514
PRIMER_RIGHT_18_PENALTY=0.504749
PRIMER_LEFT_18_SEQUENCE=ACAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_18_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_18=597,21
PRIMER_RIGHT_18=1153,20
PRIMER_LEFT_18_TM=60.625
PRIMER_RIGHT_18_TM=60.505
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=557
PRIMER_PAIR_19_PENALTY=2.151823
PRIMER_LEFT_19_PENALTY=0.578368
PRIMER_RIGHT_19_PENALTY=1.573455
PRIMER_LEFT_19_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_19_SEQUENCE=TCTTTTTGATGAATCCCCCT
PRIMER_LEFT_19=599,20
PRIMER_RIGHT_19=1160,20
PRIMER_LEFT_19_TM=60.578
PRIMER_RIGHT_19_TM=58.427
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=10.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=562
PRIMER_PAIR_20_PENALTY=2.203241
PRIMER_LEFT_20_PENALTY=1.208232
PRIMER_RIGHT_20_PENALTY=0.995010
PRIMER_LEFT_20_SEQUENCE=ACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_20_SEQUENCE=TTGATGAATCCCCCTTTTCC
PRIMER_LEFT_20=597,20
PRIMER_RIGHT_20=1155,20
PRIMER_LEFT_20_TM=58.792
PRIMER_RIGHT_20_TM=60.995
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=559
PRIMER_PAIR_21_PENALTY=2.216285
PRIMER_LEFT_21_PENALTY=0.186600
PRIMER_RIGHT_21_PENALTY=2.029685
PRIMER_LEFT_21_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_21_SEQUENCE=GTTTCAGAATCCCTCCATTCA
PRIMER_LEFT_21=598,20
PRIMER_RIGHT_21=1242,21
PRIMER_LEFT_21_TM=59.813
PRIMER_RIGHT_21_TM=58.970
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.3000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=645
PRIMER_PAIR_22_PENALTY=2.219871
PRIMER_LEFT_22_PENALTY=0.186600
PRIMER_RIGHT_22_PENALTY=2.033270
PRIMER_LEFT_22_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_22_SEQUENCE=CTTTTTGATGAATCCCCCTT
PRIMER_LEFT_22=598,20
PRIMER_RIGHT_22=1159,20
PRIMER_LEFT_22_TM=59.813
PRIMER_RIGHT_22_TM=57.967
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.3000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=562
PRIMER_PAIR_23_PENALTY=2.249932
PRIMER_LEFT_23_PENALTY=1.254922
PRIMER_RIGHT_23_PENALTY=0.995010
PRIMER_LEFT_23_SEQUENCE=TTTTGATTTTGGTGAGGCTG
PRIMER_RIGHT_23_SEQUENCE=TTGATGAATCCCCCTTTTCC
PRIMER_LEFT_23=629,20
PRIMER_RIGHT_23=1155,20
PRIMER_LEFT_23_TM=58.745
PRIMER_RIGHT_23_TM=60.995
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=527
PRIMER_PAIR_24_PENALTY=2.262946
PRIMER_LEFT_24_PENALTY=0.775718
PRIMER_RIGHT_24_PENALTY=1.487228
PRIMER_LEFT_24_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_24_SEQUENCE=GCAGAGTGATCAACGCTTTTT
PRIMER_LEFT_24=631,20
PRIMER_RIGHT_24=1268,21
PRIMER_LEFT_24_TM=60.776
PRIMER_RIGHT_24_TM=59.513
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=638
PRIMER_PAIR_25_PENALTY=2.332222
PRIMER_LEFT_25_PENALTY=0.186600
PRIMER_RIGHT_25_PENALTY=2.145621
PRIMER_LEFT_25_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_25_SEQUENCE=ATGAATCCCCCTTTTCCACC
PRIMER_LEFT_25=598,20
PRIMER_RIGHT_25=1152,20
PRIMER_LEFT_25_TM=59.813
PRIMER_RIGHT_25_TM=62.146
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=555
PRIMER_PAIR_26_PENALTY=2.349173
PRIMER_LEFT_26_PENALTY=0.775718
PRIMER_RIGHT_26_PENALTY=1.573455
PRIMER_LEFT_26_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_26_SEQUENCE=TCTTTTTGATGAATCCCCCT
PRIMER_LEFT_26=631,20
PRIMER_RIGHT_26=1160,20
PRIMER_LEFT_26_TM=60.776
PRIMER_RIGHT_26_TM=58.427
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=10.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=530
PRIMER_PAIR_27_PENALTY=2.400663
PRIMER_LEFT_27_PENALTY=1.208232
PRIMER_RIGHT_27_PENALTY=1.192432
PRIMER_LEFT_27_SEQUENCE=ACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_27_SEQUENCE=TTTGATGAATCCCCCTTTTC
PRIMER_LEFT_27=597,20
PRIMER_RIGHT_27=1156,20
PRIMER_LEFT_27_TM=58.792
PRIMER_RIGHT_27_TM=58.808
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=560
PRIMER_PAIR_28_PENALTY=2.400663
PRIMER_LEFT_28_PENALTY=1.208232
PRIMER_RIGHT_28_PENALTY=1.192432
PRIMER_LEFT_28_SEQUENCE=ACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_28_SEQUENCE=TTCTTTTTGATGAATCCCCC
PRIMER_LEFT_28=597,20
PRIMER_RIGHT_28=1161,20
PRIMER_LEFT_28_TM=58.792
PRIMER_RIGHT_28_TM=58.808
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=12.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=565
PRIMER_PAIR_29_PENALTY=2.419385
PRIMER_LEFT_29_PENALTY=0.186600
PRIMER_RIGHT_29_PENALTY=2.232784
PRIMER_LEFT_29_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_29_SEQUENCE=GAATCCCTCCATTCATATGCTC
PRIMER_LEFT_29=598,20
PRIMER_RIGHT_29=1236,22
PRIMER_LEFT_29_TM=59.813
PRIMER_RIGHT_29_TM=59.767
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.3000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=639
PRIMER_PAIR_30_PENALTY=2.447354
PRIMER_LEFT_30_PENALTY=1.254922
PRIMER_RIGHT_30_PENALTY=1.192432
PRIMER_LEFT_30_SEQUENCE=TTTTGATTTTGGTGAGGCTG
PRIMER_RIGHT_30_SEQUENCE=TTTGATGAATCCCCCTTTTC
PRIMER_LEFT_30=629,20
PRIMER_RIGHT_30=1156,20
PRIMER_LEFT_30_TM=58.745
PRIMER_RIGHT_30_TM=58.808
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=7.3000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=528
PRIMER_PAIR_31_PENALTY=2.447354
PRIMER_LEFT_31_PENALTY=1.254922
PRIMER_RIGHT_31_PENALTY=1.192432
PRIMER_LEFT_31_SEQUENCE=TTTTGATTTTGGTGAGGCTG
PRIMER_RIGHT_31_SEQUENCE=TTCTTTTTGATGAATCCCCC
PRIMER_LEFT_31=629,20
PRIMER_RIGHT_31=1161,20
PRIMER_LEFT_31_TM=58.745
PRIMER_RIGHT_31_TM=58.808
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.7000
PRIMER_RIGHT_31_END_STABILITY=12.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=533
PRIMER_PAIR_32_PENALTY=2.518011
PRIMER_LEFT_32_PENALTY=0.578368
PRIMER_RIGHT_32_PENALTY=1.939643
PRIMER_LEFT_32_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_32_SEQUENCE=TTTCAGAATCCCTCCATTCA
PRIMER_LEFT_32=599,20
PRIMER_RIGHT_32=1241,20
PRIMER_LEFT_32_TM=60.578
PRIMER_RIGHT_32_TM=58.060
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=643
PRIMER_PAIR_33_PENALTY=2.608053
PRIMER_LEFT_33_PENALTY=0.578368
PRIMER_RIGHT_33_PENALTY=2.029685
PRIMER_LEFT_33_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_33_SEQUENCE=GTTTCAGAATCCCTCCATTCA
PRIMER_LEFT_33=599,20
PRIMER_RIGHT_33=1242,21
PRIMER_LEFT_33_TM=60.578
PRIMER_RIGHT_33_TM=58.970
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=644
PRIMER_PAIR_34_PENALTY=2.611638
PRIMER_LEFT_34_PENALTY=0.578368
PRIMER_RIGHT_34_PENALTY=2.033270
PRIMER_LEFT_34_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_34_SEQUENCE=CTTTTTGATGAATCCCCCTT
PRIMER_LEFT_34=599,20
PRIMER_RIGHT_34=1159,20
PRIMER_LEFT_34_TM=60.578
PRIMER_RIGHT_34_TM=57.967
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=561
PRIMER_PAIR_35_PENALTY=2.619524
PRIMER_LEFT_35_PENALTY=1.624514
PRIMER_RIGHT_35_PENALTY=0.995010
PRIMER_LEFT_35_SEQUENCE=ACAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_35_SEQUENCE=TTGATGAATCCCCCTTTTCC
PRIMER_LEFT_35=597,21
PRIMER_RIGHT_35=1155,20
PRIMER_LEFT_35_TM=60.625
PRIMER_RIGHT_35_TM=60.995
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.3000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=559
PRIMER_PAIR_36_PENALTY=2.633852
PRIMER_LEFT_36_PENALTY=2.129103
PRIMER_RIGHT_36_PENALTY=0.504749
PRIMER_LEFT_36_SEQUENCE=TTACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_36_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_36=595,22
PRIMER_RIGHT_36=1153,20
PRIMER_LEFT_36_TM=60.129
PRIMER_RIGHT_36_TM=60.505
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=559
PRIMER_PAIR_37_PENALTY=2.657581
PRIMER_LEFT_37_PENALTY=2.152833
PRIMER_RIGHT_37_PENALTY=0.504749
PRIMER_LEFT_37_SEQUENCE=TACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_37_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_37=596,21
PRIMER_RIGHT_37=1153,20
PRIMER_LEFT_37_TM=58.847
PRIMER_RIGHT_37_TM=60.505
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=558
PRIMER_PAIR_38_PENALTY=2.657581
PRIMER_LEFT_38_PENALTY=2.152833
PRIMER_RIGHT_38_PENALTY=0.504749
PRIMER_LEFT_38_SEQUENCE=TTACAGATCGCCTGAGGTTTT
PRIMER_RIGHT_38_SEQUENCE=GATGAATCCCCCTTTTCCAC
PRIMER_LEFT_38=595,21
PRIMER_RIGHT_38=1153,20
PRIMER_LEFT_38_TM=58.847
PRIMER_RIGHT_38_TM=60.505
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=559
PRIMER_PAIR_39_PENALTY=2.665257
PRIMER_LEFT_39_PENALTY=0.186600
PRIMER_RIGHT_39_PENALTY=2.478657
PRIMER_LEFT_39_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_39_SEQUENCE=TGATGAATCCCCCTTTTCCA
PRIMER_LEFT_39=598,20
PRIMER_RIGHT_39=1154,20
PRIMER_LEFT_39_TM=59.813
PRIMER_RIGHT_39_TM=62.479
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=8.5000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=557
PRIMER_PAIR_40_PENALTY=2.695460
PRIMER_LEFT_40_PENALTY=1.208232
PRIMER_RIGHT_40_PENALTY=1.487228
PRIMER_LEFT_40_SEQUENCE=ACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_40_SEQUENCE=GCAGAGTGATCAACGCTTTTT
PRIMER_LEFT_40=597,20
PRIMER_RIGHT_40=1268,21
PRIMER_LEFT_40_TM=58.792
PRIMER_RIGHT_40_TM=59.513
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=672
PRIMER_PAIR_41_PENALTY=2.715361
PRIMER_LEFT_41_PENALTY=0.775718
PRIMER_RIGHT_41_PENALTY=1.939643
PRIMER_LEFT_41_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_41_SEQUENCE=TTTCAGAATCCCTCCATTCA
PRIMER_LEFT_41=631,20
PRIMER_RIGHT_41=1241,20
PRIMER_LEFT_41_TM=60.776
PRIMER_RIGHT_41_TM=58.060
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=611
PRIMER_PAIR_42_PENALTY=2.723990
PRIMER_LEFT_42_PENALTY=0.578368
PRIMER_RIGHT_42_PENALTY=2.145621
PRIMER_LEFT_42_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_42_SEQUENCE=ATGAATCCCCCTTTTCCACC
PRIMER_LEFT_42=599,20
PRIMER_RIGHT_42=1152,20
PRIMER_LEFT_42_TM=60.578
PRIMER_RIGHT_42_TM=62.146
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=554
PRIMER_PAIR_43_PENALTY=2.742150
PRIMER_LEFT_43_PENALTY=1.254922
PRIMER_RIGHT_43_PENALTY=1.487228
PRIMER_LEFT_43_SEQUENCE=TTTTGATTTTGGTGAGGCTG
PRIMER_RIGHT_43_SEQUENCE=GCAGAGTGATCAACGCTTTTT
PRIMER_LEFT_43=629,20
PRIMER_RIGHT_43=1268,21
PRIMER_LEFT_43_TM=58.745
PRIMER_RIGHT_43_TM=59.513
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=640
PRIMER_PAIR_44_PENALTY=2.742323
PRIMER_LEFT_44_PENALTY=0.186600
PRIMER_RIGHT_44_PENALTY=2.555723
PRIMER_LEFT_44_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_44_SEQUENCE=GCCAAAACCAAAAAGGGATACT
PRIMER_LEFT_44=598,20
PRIMER_RIGHT_44=1371,22
PRIMER_LEFT_44_TM=59.813
PRIMER_RIGHT_44_TM=60.556
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=5.3000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=774
PRIMER_PAIR_45_PENALTY=2.781686
PRIMER_LEFT_45_PENALTY=1.208232
PRIMER_RIGHT_45_PENALTY=1.573455
PRIMER_LEFT_45_SEQUENCE=ACAGATCGCCTGAGGTTTTT
PRIMER_RIGHT_45_SEQUENCE=TCTTTTTGATGAATCCCCCT
PRIMER_LEFT_45=597,20
PRIMER_RIGHT_45=1160,20
PRIMER_LEFT_45_TM=58.792
PRIMER_RIGHT_45_TM=58.427
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=10.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=564
PRIMER_PAIR_46_PENALTY=2.805403
PRIMER_LEFT_46_PENALTY=0.775718
PRIMER_RIGHT_46_PENALTY=2.029685
PRIMER_LEFT_46_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_46_SEQUENCE=GTTTCAGAATCCCTCCATTCA
PRIMER_LEFT_46=631,20
PRIMER_RIGHT_46=1242,21
PRIMER_LEFT_46_TM=60.776
PRIMER_RIGHT_46_TM=58.970
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=612
PRIMER_PAIR_47_PENALTY=2.808988
PRIMER_LEFT_47_PENALTY=0.775718
PRIMER_RIGHT_47_PENALTY=2.033270
PRIMER_LEFT_47_SEQUENCE=TTGATTTTGGTGAGGCTGCT
PRIMER_RIGHT_47_SEQUENCE=CTTTTTGATGAATCCCCCTT
PRIMER_LEFT_47=631,20
PRIMER_RIGHT_47=1159,20
PRIMER_LEFT_47_TM=60.776
PRIMER_RIGHT_47_TM=57.967
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=529
PRIMER_PAIR_48_PENALTY=2.811152
PRIMER_LEFT_48_PENALTY=0.578368
PRIMER_RIGHT_48_PENALTY=2.232784
PRIMER_LEFT_48_SEQUENCE=AGATCGCCTGAGGTTTTTCC
PRIMER_RIGHT_48_SEQUENCE=GAATCCCTCCATTCATATGCTC
PRIMER_LEFT_48=599,20
PRIMER_RIGHT_48=1236,22
PRIMER_LEFT_48_TM=60.578
PRIMER_RIGHT_48_TM=59.767
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=638
PRIMER_PAIR_49_PENALTY=2.815826
PRIMER_LEFT_49_PENALTY=0.186600
PRIMER_RIGHT_49_PENALTY=2.629225
PRIMER_LEFT_49_SEQUENCE=CAGATCGCCTGAGGTTTTTC
PRIMER_RIGHT_49_SEQUENCE=GACCACCCATGTTCTTTCTTTT
PRIMER_LEFT_49=598,20
PRIMER_RIGHT_49=1176,22
PRIMER_LEFT_49_TM=59.813
PRIMER_RIGHT_49_TM=59.371
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=579
=
SEQUENCE_ID=MAGIC.446_3_3556482_3556724_wu-0_can-0_125470_17477_TRUE
SEQUENCE_TARGET=950,342
SEQUENCE_TEMPLATE=ATAAAATTTTGGATCTTCAAAATGAAATAGTTTCTATANCTTTTTANAGTTTAATGGTTTGTGATCAACAGTTGNGTNNCANATCTAAACTCGNTTANNNNNANNAAAAANAATCTAAACTCGTTTANNNNNNNNNNNNNNCNNAAAAAANANAANAATGANAAACCGTNGCAAGTGATATCGACCTTTNGCCTACTTTCGCACTCATGTTGGTGTNTNGACNAAACCNACTGTGGACGCATATAACCACCGGANGAGTACAGGNNAAANTTTCTATTATTNNNATGAGNTTAGTTAGANAATCCAAGTCTANTAAAAGATTTGAATAANNGCAANTTGTNTCCACNAAACTAAAATAANCCAAATNCAAAGTCAGTCANNTTTGGANTTATGTTGTAATGACAGTCACTGCTTTGTCATCATNTCANCTTTTAAGNCATTGACCTTTTNTTTTTGNTCGACNAAANCCATTTTCNCATTCATCANTTTCGAATAAAANTNTATAACAGTCTAAGTAACAAANTCATNTTNGGATACCAATNTGANTTTGANTTTNATTGGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTNAAATTGTTGAATNATNTGAGTTNNNNNNNNNNNNNNNCACAAACNACAACTGNAACATATGGAANANGAAAAANANCACTTTATTTTCTTGAAAACTNTGTAAACTTNAGANTTNGTTTNTTNNNNANNNNNNNNNNNNNNNNNNNNNNNNTGAACNAAAATNCANATTTATACAGCTCAANCTGTANNTNTNTTTTTCTAAATTTAGCAACAGAAAAATACCAAATTGAANACCTTCACNNATNNNATAAAAGATAAACATGAATTGTTNNGTAAGAAATTTNATAANCCCACATGCATTTGGTCATTATTTAANAACAAAAAGNAGACNCAATTTTAATAGTATATCTTTAGATCGAATCAGCATAATGACGTATCTTTCTGACGTATAGNNNNNGTCTTNGTTGTTCTTTACNCATCTATAATAACNAAACCTGTGTGAACAAGACCTAAAACAGAGAGAAATTCACAACNAAAATGGAGACTAGNGAAACAAAACCAGTGATCTTTCTCTTCCCTTTCCCTTTACAAGGTCACTTAAACCCAATGTTTCAGCTCGCCAACATCTTCTTCAACAGAGGCTTCTCCATCACTGTGATCCACACTNAGTTCAACNCTCCAAACTCTTCCAATTTCCCTCANTTCANTTTCGTATCCATNCCCGATNGCTTGTCTGAACCTGAATNCTATCCCGATGTCATNGAGATTCTCCATGACCTCAATTCCAAGTGTGTTGCTCCTTTTGGTGATTGCTTAAAGAAGCTTATATCTGAAGAACCAACAGCAGCTTGTGTGATTGTTGACGCTCTTTGGTACTTCACTCACGATTTAACCGAGAAATTCAATTTCCCGAGGATTNTTCTCCGAACCGTTAACCTCTCAGCTTTCGTCGCTTTCTCAAAGTTTCATGTTTTACGAGAGAAAGGGTATCTTTCTTTACAAGGTACACTTTTGTTGACTTTTATATATCTCTAGTTTGCTTCATCTTTGTTGACTTTTCTTGCTCTTCTCCATATGGCAGAGACTAAGGCAGACTCACCGGTTCCGGAGCTTCCGTATCTTAGAATGAAGGATCTTCCATGGTTCCAGACAGAAGATCCAAGATCAGGGGATAAGTTACAGATAGGTGTGATGAAGTCACTAAAGTCTTCCTCAGGAATCATATTCAACGCCATTGAAGATCTTGAAACAGATCAGCTTGATGAAGCCCGCATAGAATTCCCAGTTCCACTCTTCTGTATTGGACCNTTTCACAGGTACGTTTCAGCTTCATCNAGTAGCTTACTTGCACANGACATGACTTGTCTCTCCTGGTTAGACAAGCAAGCAACAAATTCCGTAATCTACGCAAGTCTTGGAAGCATTGCTTCGATCGATGAATCTGAATTCTTGGAGATTGCTTGGGGTCTAAGAAACAGCAACCAACCTTTTCTATGGGTGGTTAGACCCGGTTTNATCCACGGGAAAGAATGG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_5_13439384_13439737_ws-0_ct-1_189291_461661_TRUE
SEQUENCE_TARGET=950,453
SEQUENCE_TEMPLATE=TGTTCTTTCTTCTCTGCTCATTGTTATGGTTTTTATACTATATTATATGATTCTTGAAGAAAATCGTGCTTGAGTTTTAGATATGAGGGGGTCATATTTTCGAGAACAAAAACAGAAAAGGAAATGAAAGGGANAAAANNAGCAAATACTCTCTTCAATANAACCTTCATATAAAACATATATACCGTAGAAATTCTAAATTCTAACAAAAGATTCGTTGTAGCACATTGTGTAAAGCAAACATTCTAGTTTAATACTGGATCAGAATTACGGGTTTGAAGCTCGGCAGCGGAAANANCNTTTTGGTGCTTTTNATACTTTATCTTGTATGGCAATTNAAGATACTTTCTCGTATTGATCTTTCTTCTTTACTCATTGTTATAGTTTTTATACTATATTATATGATTCTTGAAGAAANTCGTGCTTNANTTTTAGATATNAGAGGGNTCATATTTTTGAGANCAAAAACAGAAAANGAANTGAATGGGAGAAAATAAGCAAATACNCNTTTNNNNNNNNNNNNNANATAAAACATATATACCGTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTNTAAATTATAACAAAAGATCCGCTNTANCACATTGTCTAAAGCAAACATTCTAGTTTAATACTGGATCAGANTTNCGGGTTCGAAGCCCGCANCGAAAAAATCTTTNTGGTGGTTTTTATNAAGTATCTTGTTTGAAAATTGAAGATACTTTCTCGTATTGTTCTTTCTTCTCTGCTCATTGTTATGGTTTTTATACTATATTTTATGATTCTTGAAGAAAATCGTGCTTGAGTTTTAGATATGAGGGGTTCATATTTTCGAGAACAAAAATAGAAAAGGAAATGAATGGGAGAAAAGAAGCAAATATTCTTTTCAATAAAGCCTTCACATAAAACATATATTCTGTATAAATTCTAAATTCTAACAAAAGATCCGCTGTAACACATTNTCGAAAGCAATCATTCTAGTTTAATACTAGATAAGAATTCCNTTTTCNAAGCCCGGNAGCGGAAAAATCCCTTTGGTGCTTTTTATAATGTATCTTGTATGACAATTGAAGATACTTTATCANATTNTTCTTTNTTCTCTGCTCATTGTTATCGTTTTTATACTATATTATATGATTCTTGAAGAAAATCGTGCTTGAGTTTTAGATATGAGGGGTTCATATCTTCGAGAGCAAAANTAGAAAANGAAATGAATGGGAGAAAAGAAGCAAATACTNTTTTCAATAAAACCTTCCCATAAAACATATATTCCGTATAAATTCTAAATTCTAACAAAAGATCCNATGTAGCACATTGTCGAAAGCAATCATTCTAGTTTAATACTGGATCAGAATTCNNGGTTCGAAGCCNGGCANCGAAGAAATCCTTTTGATGCTTTTTATNATGTATCTTGAATGACAATTGAAGATACTTTCTCATATTGTTCTTTCTTCTTTTCTCATTGTTATGGTTTTTATACTATATTATATGATTCTTGAAGAAAACCGTGTNTGAGTTTTATATGAGGGGGTCATATTTTCGAGAACAAAAACAGAAAAGGAAATGAAAGGGAGNAAAGAAGCAAATACTCTCTTCAATAAAACCTTCACATAAAACATANATACCGTAGAAATTCTAAATTCTAACAAAAGNTCCGTTGTAGCNCATTGTCTAAAGCAAACATTCTAGTTTAATACTGGATCAGAATTACGCGTTTGAAGCCNGANAGCNNAAAAATCCTTTTGGTGCTTTTTATAATNTATCTTGTATGGCAATTNAANATGTATTGATCTTTNTTCTTTGCTCATTGTTATGGTTTNTATATTNTATTATATGATTCTTGAAAAAAATCGTGCTTGAGTTTTAGATATGAGAGGAGTCATATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_11334855_11334973_zu-0_wil-2_230889_213531_TRUE
SEQUENCE_TARGET=950,218
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_10481737_10481745_zu-0_wil-2_1219_396155_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_13862974_13863094_hi-0_ler-0_57024_29340_TRUE
SEQUENCE_TARGET=950,220
SEQUENCE_TEMPLATE=TAACTCATACAAATCAATCATCGACCATACAAAGTAACCTCTCGTGTCCGATCCATTCCTGATCAGTAAGTAAAACTGGAATTAGACCATGAATCNTAATGTAGTAANATGAGGACTAAAAGATGNAGAAGGAGNATCATACTTGATGGCNTTGAGCANAGCACCAATGTAAGCTTGAATGAATTCAATTCTTTGTGTGTCTTGTAATGTCGAGTCGCGTCCCATCGGCATACCTGTTTTTATATTTAAAAAATTGCAAGTTGAAGAATCTTAAAGAACAAGAAAGTGTGCTTTCAAGCAGACACAACAGAGTAAGAAAACAAAAGAGAATGAAGTGTTGTACCATTTTCAAGAATATAGATTGGAGGATTGTTATAGCTCTGCTTTANATACTCAAGAANANCTTCAAGACCCCATGGAGTAGCCTCCCACTTCAAGAAAAACAATTGATTCAAGAAGAGCTTTTGGCAAAAAATATATATGTATTATGCAAATTTGCAGGACCTTACCAGAAGAAATGAAGAATTCGCAGCGGCTGTCCAAAGAAGAAGAACTATTAGTTTTAAGAATTGATCAGAAGCATTTTTCTATTGGTTAATGATGAAAGAAAACTAATCTTACAAATCATATATACTCCCATGTCTTTAAAGAAGCCTTCATTCATGCTGGGAAAGATAGAAGGTGAGGGTTTGTTTGTGACATAAAATGTCGTGTAGTGAATAATTCCTANAAAGTCAGATGATCCTTTAANTTGCTCTGACTCTTCCTCCGAAAACACCGGTAATCTCGATCCAACAGTTCTCTTCATTTCATCCGGATAGTCTCCAAATACCAAAGGCTTCAACATCCTGAATTATCATTTTAGTCTTCAGATTTTGTCCGCAAGTTATTGAAACACAGAGAAAGAAAGCTTTCTTTTTGCTCACCAGCCATAGAAGAAAGCTTTAGCTCTTTGAGTTGCGATTTCATCNTCNTTGGAGTTTGTATANGGAGATAACCCNAATGCAAATATACTAAGCCCTATGGATCCTTTCTGCNTACTCTGCACCATCATNATGAACNNTGATCATACGAGGTTNAGGNNTNAAGAGCNAAAAATGTGTNTATATACCTTGTACTTNAGTTTATACAACTTTGAAGNAGAGGCATGAGCTAGCAATATGTTATGGCCTGCAAGATATGGTTCNGTAGAAGAATTTCCACTAGTACAATTGATGAATTTGTTAGGAGAACAATGTCCAGGGGGCGAGATTCCTTGGTCATAAGAACCAATGGCGAAAATTGTAGCTTCGTTTATTGTAGTCCATAACTTCACATCCTCCCCAAACTCTCTGAANCATACATCTGCATAAGCAGTGAAGTCTTCTCTGCAATATATAAAAACAAACACCATTTTTATTTACANTTCATATTTAAAAATTGACTTNGCTAGATCANAATCTTTTTTTCTTTTTATCCTTTACATGATTTTGCGGTTGATCCAGCCTCCATACTCATCTTCAAGAGACTGAGGAAGATCATAGTGGTAAAGNGTAACATGTGGTTCGATTCCTGTTAATAGTTTTGCCAAAATTTTAATTAGAAGCTATAAACTGGCTTTAGTAGCGATGTAAACCAAAAAANCTCACCATGGCTTATTAGTTCTTTGATGAGNTTCTTGTAAAACAGTAGACCTTTTGGNTTAATGAGTCCNCTTCCATCTGNNNNNNNNNNNNNACAAAAAGNGTTTAAGAGGTCAGAAACAACAANATCGTTACTTGCAAATAAAGAAACAAGGAAGAGGGTGTTACTAGGTATAAGTCTTGACCAGGAGATAGAGAATCTGAATGATTCTAAGCCCATTTCTGCCATCAGCTTAACATCTTCCTGCCAAAGTATCAACAACATCGAAATTGGAAAACAGAGACTTAACGGTAACTTCTTCCAAAATTTAACGGCAAAGAAGAGAGAGAGGAACCTTGTATTTGTGATANCCATCGGATGTTATATCTCCATTACCTAAATTACCTCTATTGTCTGCAAGTTTCGAACAAGAATCTATCAGAAATTTTCTCATATAAATCCCCTAATAATGAACACAAAATTTCGAAATAGGCAATTATATTGAACCACAGAGTAAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.462063
PRIMER_LEFT_0_PENALTY=0.365170
PRIMER_RIGHT_0_PENALTY=0.096893
PRIMER_LEFT_0_SEQUENCE=TTTCTTTTTGCTCACCAGCC
PRIMER_RIGHT_0_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_0=911,20
PRIMER_RIGHT_0=1329,20
PRIMER_LEFT_0_TM=60.365
PRIMER_RIGHT_0_TM=59.903
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=419
PRIMER_PAIR_1_PENALTY=0.543694
PRIMER_LEFT_1_PENALTY=0.507521
PRIMER_RIGHT_1_PENALTY=0.036173
PRIMER_LEFT_1_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_1_SEQUENCE=TATGACCAAGGAATCTCGCC
PRIMER_LEFT_1=532,20
PRIMER_RIGHT_1=1263,20
PRIMER_LEFT_1_TM=60.508
PRIMER_RIGHT_1_TM=60.036
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=732
PRIMER_PAIR_2_PENALTY=0.550315
PRIMER_LEFT_2_PENALTY=0.453422
PRIMER_RIGHT_2_PENALTY=0.096893
PRIMER_LEFT_2_SEQUENCE=TGCTCACCAGCCATAGAAGA
PRIMER_RIGHT_2_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_2=919,20
PRIMER_RIGHT_2=1329,20
PRIMER_LEFT_2_TM=59.547
PRIMER_RIGHT_2_TM=59.903
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=411
PRIMER_PAIR_3_PENALTY=0.569482
PRIMER_LEFT_3_PENALTY=0.507521
PRIMER_RIGHT_3_PENALTY=0.061961
PRIMER_LEFT_3_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_3_SEQUENCE=CAATTTTCGCCATTGGTTCT
PRIMER_LEFT_3=532,20
PRIMER_RIGHT_3=1283,20
PRIMER_LEFT_3_TM=60.508
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=752
PRIMER_PAIR_4_PENALTY=0.604414
PRIMER_LEFT_4_PENALTY=0.507521
PRIMER_RIGHT_4_PENALTY=0.096893
PRIMER_LEFT_4_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_4_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_4=532,20
PRIMER_RIGHT_4=1329,20
PRIMER_LEFT_4_TM=60.508
PRIMER_RIGHT_4_TM=59.903
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=798
PRIMER_PAIR_5_PENALTY=0.677060
PRIMER_LEFT_5_PENALTY=0.580167
PRIMER_RIGHT_5_PENALTY=0.096893
PRIMER_LEFT_5_SEQUENCE=TTTGCTCACCAGCCATAGAA
PRIMER_RIGHT_5_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_5=917,20
PRIMER_RIGHT_5=1329,20
PRIMER_LEFT_5_TM=59.420
PRIMER_RIGHT_5_TM=59.903
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.0000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=413
PRIMER_PAIR_6_PENALTY=0.677060
PRIMER_LEFT_6_PENALTY=0.580167
PRIMER_RIGHT_6_PENALTY=0.096893
PRIMER_LEFT_6_SEQUENCE=TTTTGCTCACCAGCCATAGA
PRIMER_RIGHT_6_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_6=916,20
PRIMER_RIGHT_6=1329,20
PRIMER_LEFT_6_TM=59.420
PRIMER_RIGHT_6_TM=59.903
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=414
PRIMER_PAIR_7_PENALTY=0.697199
PRIMER_LEFT_7_PENALTY=0.365170
PRIMER_RIGHT_7_PENALTY=0.332028
PRIMER_LEFT_7_SEQUENCE=TTTCTTTTTGCTCACCAGCC
PRIMER_RIGHT_7_SEQUENCE=ATGGCAGAAATGGGCTTAGA
PRIMER_LEFT_7=911,20
PRIMER_RIGHT_7=1850,20
PRIMER_LEFT_7_TM=60.365
PRIMER_RIGHT_7_TM=59.668
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=6.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=940
PRIMER_PAIR_8_PENALTY=0.700654
PRIMER_LEFT_8_PENALTY=0.507521
PRIMER_RIGHT_8_PENALTY=0.193133
PRIMER_LEFT_8_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_8_SEQUENCE=ACAATTTTCGCCATTGGTTC
PRIMER_LEFT_8=532,20
PRIMER_RIGHT_8=1284,20
PRIMER_LEFT_8_TM=60.508
PRIMER_RIGHT_8_TM=59.807
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=753
PRIMER_PAIR_9_PENALTY=0.716318
PRIMER_LEFT_9_PENALTY=0.365170
PRIMER_RIGHT_9_PENALTY=0.351148
PRIMER_LEFT_9_SEQUENCE=TTTCTTTTTGCTCACCAGCC
PRIMER_RIGHT_9_SEQUENCE=CAGAGAGTTTGGGGAGGATG
PRIMER_LEFT_9=911,20
PRIMER_RIGHT_9=1333,20
PRIMER_LEFT_9_TM=60.365
PRIMER_RIGHT_9_TM=59.649
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=423
PRIMER_PAIR_10_PENALTY=0.734795
PRIMER_LEFT_10_PENALTY=0.507521
PRIMER_RIGHT_10_PENALTY=0.227274
PRIMER_LEFT_10_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_10_SEQUENCE=TTCAGAGAGTTTGGGGAGGA
PRIMER_LEFT_10=532,20
PRIMER_RIGHT_10=1335,20
PRIMER_LEFT_10_TM=60.508
PRIMER_RIGHT_10_TM=59.773
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=804
PRIMER_PAIR_11_PENALTY=0.747893
PRIMER_LEFT_11_PENALTY=0.711720
PRIMER_RIGHT_11_PENALTY=0.036173
PRIMER_LEFT_11_SEQUENCE=GCCTCCCACTTCAAGAAAAA
PRIMER_RIGHT_11_SEQUENCE=TATGACCAAGGAATCTCGCC
PRIMER_LEFT_11=423,20
PRIMER_RIGHT_11=1263,20
PRIMER_LEFT_11_TM=59.288
PRIMER_RIGHT_11_TM=60.036
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=841
PRIMER_PAIR_12_PENALTY=0.773681
PRIMER_LEFT_12_PENALTY=0.711720
PRIMER_RIGHT_12_PENALTY=0.061961
PRIMER_LEFT_12_SEQUENCE=GCCTCCCACTTCAAGAAAAA
PRIMER_RIGHT_12_SEQUENCE=CAATTTTCGCCATTGGTTCT
PRIMER_LEFT_12=423,20
PRIMER_RIGHT_12=1283,20
PRIMER_LEFT_12_TM=59.288
PRIMER_RIGHT_12_TM=59.938
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=861
PRIMER_PAIR_13_PENALTY=0.785450
PRIMER_LEFT_13_PENALTY=0.453422
PRIMER_RIGHT_13_PENALTY=0.332028
PRIMER_LEFT_13_SEQUENCE=TGCTCACCAGCCATAGAAGA
PRIMER_RIGHT_13_SEQUENCE=ATGGCAGAAATGGGCTTAGA
PRIMER_LEFT_13=919,20
PRIMER_RIGHT_13=1850,20
PRIMER_LEFT_13_TM=59.547
PRIMER_RIGHT_13_TM=59.668
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=932
PRIMER_PAIR_14_PENALTY=0.804569
PRIMER_LEFT_14_PENALTY=0.453422
PRIMER_RIGHT_14_PENALTY=0.351148
PRIMER_LEFT_14_SEQUENCE=TGCTCACCAGCCATAGAAGA
PRIMER_RIGHT_14_SEQUENCE=CAGAGAGTTTGGGGAGGATG
PRIMER_LEFT_14=919,20
PRIMER_RIGHT_14=1333,20
PRIMER_LEFT_14_TM=59.547
PRIMER_RIGHT_14_TM=59.649
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=415
PRIMER_PAIR_15_PENALTY=0.807441
PRIMER_LEFT_15_PENALTY=0.580167
PRIMER_RIGHT_15_PENALTY=0.227274
PRIMER_LEFT_15_SEQUENCE=TTTGCTCACCAGCCATAGAA
PRIMER_RIGHT_15_SEQUENCE=TTCAGAGAGTTTGGGGAGGA
PRIMER_LEFT_15=917,20
PRIMER_RIGHT_15=1335,20
PRIMER_LEFT_15_TM=59.420
PRIMER_RIGHT_15_TM=59.773
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=419
PRIMER_PAIR_16_PENALTY=0.807441
PRIMER_LEFT_16_PENALTY=0.580167
PRIMER_RIGHT_16_PENALTY=0.227274
PRIMER_LEFT_16_SEQUENCE=TTTTGCTCACCAGCCATAGA
PRIMER_RIGHT_16_SEQUENCE=TTCAGAGAGTTTGGGGAGGA
PRIMER_LEFT_16=916,20
PRIMER_RIGHT_16=1335,20
PRIMER_LEFT_16_TM=59.420
PRIMER_RIGHT_16_TM=59.773
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=420
PRIMER_PAIR_17_PENALTY=0.808613
PRIMER_LEFT_17_PENALTY=0.711720
PRIMER_RIGHT_17_PENALTY=0.096893
PRIMER_LEFT_17_SEQUENCE=GCCTCCCACTTCAAGAAAAA
PRIMER_RIGHT_17_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_17=423,20
PRIMER_RIGHT_17=1329,20
PRIMER_LEFT_17_TM=59.288
PRIMER_RIGHT_17_TM=59.903
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=907
PRIMER_PAIR_18_PENALTY=0.858669
PRIMER_LEFT_18_PENALTY=0.507521
PRIMER_RIGHT_18_PENALTY=0.351148
PRIMER_LEFT_18_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_18_SEQUENCE=CAGAGAGTTTGGGGAGGATG
PRIMER_LEFT_18=532,20
PRIMER_RIGHT_18=1333,20
PRIMER_LEFT_18_TM=60.508
PRIMER_RIGHT_18_TM=59.649
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=802
PRIMER_PAIR_19_PENALTY=0.859981
PRIMER_LEFT_19_PENALTY=0.365170
PRIMER_RIGHT_19_PENALTY=0.494811
PRIMER_LEFT_19_SEQUENCE=TTTCTTTTTGCTCACCAGCC
PRIMER_RIGHT_19_SEQUENCE=GAGAGTTTGGGGAGGATGTG
PRIMER_LEFT_19=911,20
PRIMER_RIGHT_19=1331,20
PRIMER_LEFT_19_TM=60.365
PRIMER_RIGHT_19_TM=59.505
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=421
PRIMER_PAIR_20_PENALTY=0.874109
PRIMER_LEFT_20_PENALTY=0.837936
PRIMER_RIGHT_20_PENALTY=0.036173
PRIMER_LEFT_20_SEQUENCE=CATGGAGTAGCCTCCCACTT
PRIMER_RIGHT_20_SEQUENCE=TATGACCAAGGAATCTCGCC
PRIMER_LEFT_20=414,20
PRIMER_RIGHT_20=1263,20
PRIMER_LEFT_20_TM=59.162
PRIMER_RIGHT_20_TM=60.036
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=850
PRIMER_PAIR_21_PENALTY=0.889249
PRIMER_LEFT_21_PENALTY=0.853077
PRIMER_RIGHT_21_PENALTY=0.036173
PRIMER_LEFT_21_SEQUENCE=CCGGTAATCTCGATCCAACA
PRIMER_RIGHT_21_SEQUENCE=TATGACCAAGGAATCTCGCC
PRIMER_LEFT_21=777,20
PRIMER_RIGHT_21=1263,20
PRIMER_LEFT_21_TM=60.853
PRIMER_RIGHT_21_TM=60.036
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=11.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=487
PRIMER_PAIR_22_PENALTY=0.899897
PRIMER_LEFT_22_PENALTY=0.837936
PRIMER_RIGHT_22_PENALTY=0.061961
PRIMER_LEFT_22_SEQUENCE=CATGGAGTAGCCTCCCACTT
PRIMER_RIGHT_22_SEQUENCE=CAATTTTCGCCATTGGTTCT
PRIMER_LEFT_22=414,20
PRIMER_RIGHT_22=1283,20
PRIMER_LEFT_22_TM=59.162
PRIMER_RIGHT_22_TM=59.938
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=870
PRIMER_PAIR_23_PENALTY=0.904852
PRIMER_LEFT_23_PENALTY=0.711720
PRIMER_RIGHT_23_PENALTY=0.193133
PRIMER_LEFT_23_SEQUENCE=GCCTCCCACTTCAAGAAAAA
PRIMER_RIGHT_23_SEQUENCE=ACAATTTTCGCCATTGGTTC
PRIMER_LEFT_23=423,20
PRIMER_RIGHT_23=1284,20
PRIMER_LEFT_23_TM=59.288
PRIMER_RIGHT_23_TM=59.807
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=862
PRIMER_PAIR_24_PENALTY=0.912195
PRIMER_LEFT_24_PENALTY=0.580167
PRIMER_RIGHT_24_PENALTY=0.332028
PRIMER_LEFT_24_SEQUENCE=TTTGCTCACCAGCCATAGAA
PRIMER_RIGHT_24_SEQUENCE=ATGGCAGAAATGGGCTTAGA
PRIMER_LEFT_24=917,20
PRIMER_RIGHT_24=1850,20
PRIMER_LEFT_24_TM=59.420
PRIMER_RIGHT_24_TM=59.668
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.0000
PRIMER_RIGHT_24_END_STABILITY=6.0000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=934
PRIMER_PAIR_25_PENALTY=0.912195
PRIMER_LEFT_25_PENALTY=0.580167
PRIMER_RIGHT_25_PENALTY=0.332028
PRIMER_LEFT_25_SEQUENCE=TTTTGCTCACCAGCCATAGA
PRIMER_RIGHT_25_SEQUENCE=ATGGCAGAAATGGGCTTAGA
PRIMER_LEFT_25=916,20
PRIMER_RIGHT_25=1850,20
PRIMER_LEFT_25_TM=59.420
PRIMER_RIGHT_25_TM=59.668
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=5.6000
PRIMER_RIGHT_25_END_STABILITY=6.0000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=935
PRIMER_PAIR_26_PENALTY=0.915038
PRIMER_LEFT_26_PENALTY=0.853077
PRIMER_RIGHT_26_PENALTY=0.061961
PRIMER_LEFT_26_SEQUENCE=CCGGTAATCTCGATCCAACA
PRIMER_RIGHT_26_SEQUENCE=CAATTTTCGCCATTGGTTCT
PRIMER_LEFT_26=777,20
PRIMER_RIGHT_26=1283,20
PRIMER_LEFT_26_TM=60.853
PRIMER_RIGHT_26_TM=59.938
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=507
PRIMER_PAIR_27_PENALTY=0.931314
PRIMER_LEFT_27_PENALTY=0.580167
PRIMER_RIGHT_27_PENALTY=0.351148
PRIMER_LEFT_27_SEQUENCE=TTTGCTCACCAGCCATAGAA
PRIMER_RIGHT_27_SEQUENCE=CAGAGAGTTTGGGGAGGATG
PRIMER_LEFT_27=917,20
PRIMER_RIGHT_27=1333,20
PRIMER_LEFT_27_TM=59.420
PRIMER_RIGHT_27_TM=59.649
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.0000
PRIMER_RIGHT_27_END_STABILITY=8.1000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=417
PRIMER_PAIR_28_PENALTY=0.931314
PRIMER_LEFT_28_PENALTY=0.580167
PRIMER_RIGHT_28_PENALTY=0.351148
PRIMER_LEFT_28_SEQUENCE=TTTTGCTCACCAGCCATAGA
PRIMER_RIGHT_28_SEQUENCE=CAGAGAGTTTGGGGAGGATG
PRIMER_LEFT_28=916,20
PRIMER_RIGHT_28=1333,20
PRIMER_LEFT_28_TM=59.420
PRIMER_RIGHT_28_TM=59.649
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=5.6000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=418
PRIMER_PAIR_29_PENALTY=0.934829
PRIMER_LEFT_29_PENALTY=0.837936
PRIMER_RIGHT_29_PENALTY=0.096893
PRIMER_LEFT_29_SEQUENCE=CATGGAGTAGCCTCCCACTT
PRIMER_RIGHT_29_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_29=414,20
PRIMER_RIGHT_29=1329,20
PRIMER_LEFT_29_TM=59.162
PRIMER_RIGHT_29_TM=59.903
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=916
PRIMER_PAIR_30_PENALTY=0.948232
PRIMER_LEFT_30_PENALTY=0.453422
PRIMER_RIGHT_30_PENALTY=0.494811
PRIMER_LEFT_30_SEQUENCE=TGCTCACCAGCCATAGAAGA
PRIMER_RIGHT_30_SEQUENCE=AGAGTTTGGGGAGGATGTGA
PRIMER_LEFT_30=919,20
PRIMER_RIGHT_30=1330,20
PRIMER_LEFT_30_TM=59.547
PRIMER_RIGHT_30_TM=59.505
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=412
PRIMER_PAIR_31_PENALTY=0.948232
PRIMER_LEFT_31_PENALTY=0.453422
PRIMER_RIGHT_31_PENALTY=0.494811
PRIMER_LEFT_31_SEQUENCE=TGCTCACCAGCCATAGAAGA
PRIMER_RIGHT_31_SEQUENCE=GAGAGTTTGGGGAGGATGTG
PRIMER_LEFT_31=919,20
PRIMER_RIGHT_31=1331,20
PRIMER_LEFT_31_TM=59.547
PRIMER_RIGHT_31_TM=59.505
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=413
PRIMER_PAIR_32_PENALTY=0.949970
PRIMER_LEFT_32_PENALTY=0.853077
PRIMER_RIGHT_32_PENALTY=0.096893
PRIMER_LEFT_32_SEQUENCE=CCGGTAATCTCGATCCAACA
PRIMER_RIGHT_32_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_32=777,20
PRIMER_RIGHT_32=1329,20
PRIMER_LEFT_32_TM=60.853
PRIMER_RIGHT_32_TM=59.903
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=553
PRIMER_PAIR_33_PENALTY=1.002332
PRIMER_LEFT_33_PENALTY=0.507521
PRIMER_RIGHT_33_PENALTY=0.494811
PRIMER_LEFT_33_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_33_SEQUENCE=AGAGTTTGGGGAGGATGTGA
PRIMER_LEFT_33=532,20
PRIMER_RIGHT_33=1330,20
PRIMER_LEFT_33_TM=60.508
PRIMER_RIGHT_33_TM=59.505
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=799
PRIMER_PAIR_34_PENALTY=1.002332
PRIMER_LEFT_34_PENALTY=0.507521
PRIMER_RIGHT_34_PENALTY=0.494811
PRIMER_LEFT_34_SEQUENCE=CGGCTGTCCAAAGAAGAAGA
PRIMER_RIGHT_34_SEQUENCE=GAGAGTTTGGGGAGGATGTG
PRIMER_LEFT_34=532,20
PRIMER_RIGHT_34=1331,20
PRIMER_LEFT_34_TM=60.508
PRIMER_RIGHT_34_TM=59.505
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=6.6000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=800
PRIMER_PAIR_35_PENALTY=1.007776
PRIMER_LEFT_35_PENALTY=0.971603
PRIMER_RIGHT_35_PENALTY=0.036173
PRIMER_LEFT_35_SEQUENCE=CCTTCATTCATGCTGGGAAA
PRIMER_RIGHT_35_SEQUENCE=TATGACCAAGGAATCTCGCC
PRIMER_LEFT_35=653,20
PRIMER_RIGHT_35=1263,20
PRIMER_LEFT_35_TM=60.972
PRIMER_RIGHT_35_TM=60.036
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=11.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=611
PRIMER_PAIR_36_PENALTY=1.031069
PRIMER_LEFT_36_PENALTY=0.837936
PRIMER_RIGHT_36_PENALTY=0.193133
PRIMER_LEFT_36_SEQUENCE=CATGGAGTAGCCTCCCACTT
PRIMER_RIGHT_36_SEQUENCE=ACAATTTTCGCCATTGGTTC
PRIMER_LEFT_36=414,20
PRIMER_RIGHT_36=1284,20
PRIMER_LEFT_36_TM=59.162
PRIMER_RIGHT_36_TM=59.807
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=871
PRIMER_PAIR_37_PENALTY=1.033564
PRIMER_LEFT_37_PENALTY=0.971603
PRIMER_RIGHT_37_PENALTY=0.061961
PRIMER_LEFT_37_SEQUENCE=CCTTCATTCATGCTGGGAAA
PRIMER_RIGHT_37_SEQUENCE=CAATTTTCGCCATTGGTTCT
PRIMER_LEFT_37=653,20
PRIMER_RIGHT_37=1283,20
PRIMER_LEFT_37_TM=60.972
PRIMER_RIGHT_37_TM=59.938
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=631
PRIMER_PAIR_38_PENALTY=1.046209
PRIMER_LEFT_38_PENALTY=0.853077
PRIMER_RIGHT_38_PENALTY=0.193133
PRIMER_LEFT_38_SEQUENCE=CCGGTAATCTCGATCCAACA
PRIMER_RIGHT_38_SEQUENCE=ACAATTTTCGCCATTGGTTC
PRIMER_LEFT_38=777,20
PRIMER_RIGHT_38=1284,20
PRIMER_LEFT_38_TM=60.853
PRIMER_RIGHT_38_TM=59.807
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=508
PRIMER_PAIR_39_PENALTY=1.062868
PRIMER_LEFT_39_PENALTY=0.711720
PRIMER_RIGHT_39_PENALTY=0.351148
PRIMER_LEFT_39_SEQUENCE=GCCTCCCACTTCAAGAAAAA
PRIMER_RIGHT_39_SEQUENCE=CAGAGAGTTTGGGGAGGATG
PRIMER_LEFT_39=423,20
PRIMER_RIGHT_39=1333,20
PRIMER_LEFT_39_TM=59.288
PRIMER_RIGHT_39_TM=59.649
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=911
PRIMER_PAIR_40_PENALTY=1.065210
PRIMER_LEFT_40_PENALTY=0.837936
PRIMER_RIGHT_40_PENALTY=0.227274
PRIMER_LEFT_40_SEQUENCE=CATGGAGTAGCCTCCCACTT
PRIMER_RIGHT_40_SEQUENCE=TTCAGAGAGTTTGGGGAGGA
PRIMER_LEFT_40=414,20
PRIMER_RIGHT_40=1335,20
PRIMER_LEFT_40_TM=59.162
PRIMER_RIGHT_40_TM=59.773
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=922
PRIMER_PAIR_41_PENALTY=1.068496
PRIMER_LEFT_41_PENALTY=0.971603
PRIMER_RIGHT_41_PENALTY=0.096893
PRIMER_LEFT_41_SEQUENCE=CCTTCATTCATGCTGGGAAA
PRIMER_RIGHT_41_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_41=653,20
PRIMER_RIGHT_41=1329,20
PRIMER_LEFT_41_TM=60.972
PRIMER_RIGHT_41_TM=59.903
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=677
PRIMER_PAIR_42_PENALTY=1.072510
PRIMER_LEFT_42_PENALTY=0.975617
PRIMER_RIGHT_42_PENALTY=0.096893
PRIMER_LEFT_42_SEQUENCE=TTGCTCACCAGCCATAGAAG
PRIMER_RIGHT_42_SEQUENCE=GAGTTTGGGGAGGATGTGAA
PRIMER_LEFT_42=918,20
PRIMER_RIGHT_42=1329,20
PRIMER_LEFT_42_TM=59.024
PRIMER_RIGHT_42_TM=59.903
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=412
PRIMER_PAIR_43_PENALTY=1.074977
PRIMER_LEFT_43_PENALTY=0.580167
PRIMER_RIGHT_43_PENALTY=0.494811
PRIMER_LEFT_43_SEQUENCE=TTTGCTCACCAGCCATAGAA
PRIMER_RIGHT_43_SEQUENCE=AGAGTTTGGGGAGGATGTGA
PRIMER_LEFT_43=917,20
PRIMER_RIGHT_43=1330,20
PRIMER_LEFT_43_TM=59.420
PRIMER_RIGHT_43_TM=59.505
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.0000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=414
PRIMER_PAIR_44_PENALTY=1.074977
PRIMER_LEFT_44_PENALTY=0.580167
PRIMER_RIGHT_44_PENALTY=0.494811
PRIMER_LEFT_44_SEQUENCE=TTTGCTCACCAGCCATAGAA
PRIMER_RIGHT_44_SEQUENCE=GAGAGTTTGGGGAGGATGTG
PRIMER_LEFT_44=917,20
PRIMER_RIGHT_44=1331,20
PRIMER_LEFT_44_TM=59.420
PRIMER_RIGHT_44_TM=59.505
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.0000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=415
PRIMER_PAIR_45_PENALTY=1.074977
PRIMER_LEFT_45_PENALTY=0.580167
PRIMER_RIGHT_45_PENALTY=0.494811
PRIMER_LEFT_45_SEQUENCE=TTTTGCTCACCAGCCATAGA
PRIMER_RIGHT_45_SEQUENCE=AGAGTTTGGGGAGGATGTGA
PRIMER_LEFT_45=916,20
PRIMER_RIGHT_45=1330,20
PRIMER_LEFT_45_TM=59.420
PRIMER_RIGHT_45_TM=59.505
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=5.6000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=415
PRIMER_PAIR_46_PENALTY=1.074977
PRIMER_LEFT_46_PENALTY=0.580167
PRIMER_RIGHT_46_PENALTY=0.494811
PRIMER_LEFT_46_SEQUENCE=TTTTGCTCACCAGCCATAGA
PRIMER_RIGHT_46_SEQUENCE=GAGAGTTTGGGGAGGATGTG
PRIMER_LEFT_46=916,20
PRIMER_RIGHT_46=1331,20
PRIMER_LEFT_46_TM=59.420
PRIMER_RIGHT_46_TM=59.505
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=5.6000
PRIMER_RIGHT_46_END_STABILITY=6.6000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=416
PRIMER_PAIR_47_PENALTY=1.080350
PRIMER_LEFT_47_PENALTY=0.853077
PRIMER_RIGHT_47_PENALTY=0.227274
PRIMER_LEFT_47_SEQUENCE=CCGGTAATCTCGATCCAACA
PRIMER_RIGHT_47_SEQUENCE=TTCAGAGAGTTTGGGGAGGA
PRIMER_LEFT_47=777,20
PRIMER_RIGHT_47=1335,20
PRIMER_LEFT_47_TM=60.853
PRIMER_RIGHT_47_TM=59.773
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=559
PRIMER_PAIR_48_PENALTY=1.127412
PRIMER_LEFT_48_PENALTY=1.091240
PRIMER_RIGHT_48_PENALTY=0.036173
PRIMER_LEFT_48_SEQUENCE=AGCCTCCCACTTCAAGAAAA
PRIMER_RIGHT_48_SEQUENCE=TATGACCAAGGAATCTCGCC
PRIMER_LEFT_48=422,20
PRIMER_RIGHT_48=1263,20
PRIMER_LEFT_48_TM=58.909
PRIMER_RIGHT_48_TM=60.036
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=11.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=842
PRIMER_PAIR_49_PENALTY=1.138736
PRIMER_LEFT_49_PENALTY=1.102564
PRIMER_RIGHT_49_PENALTY=0.036173
PRIMER_LEFT_49_SEQUENCE=GCCTCCCACTTCAAGAAAAAC
PRIMER_RIGHT_49_SEQUENCE=TATGACCAAGGAATCTCGCC
PRIMER_LEFT_49=423,21
PRIMER_RIGHT_49=1263,20
PRIMER_LEFT_49_TM=60.103
PRIMER_RIGHT_49_TM=60.036
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=11.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=841
=
SEQUENCE_ID=MAGIC.105_4_10398801_10398900_po-0_bur-0_6285_54730_TRUE
SEQUENCE_TARGET=950,199
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAAATAAAAAGAGTGTTTTGGACAAAAGAAACTCCCGAAGAAGTTAGACANTAAAAGCTAATAATAGACGCAATGGAAAAACCNATCTTTTAAGGNTTNAAGTNNTTAACCATGGGAACTCAANCTTTNATTTATCCCNTTTTTTTCTTGTCCGAAAGCAACAAATCAATGATCAGCCGTTAATTNACGCCGCCACGTAATCGCGCGAAGGAGAACGACGGCAGTTATAGATTAAACAAACACGTGNCACCGGCACACGCTAGACGGNAGCTTTGTGANNTTAACTTTGGCTGACGTTAAGCTAGTTTTCGACACGACACGTGTTANCGCACACNTGTGGGTTCTCNTNTTTTTTGTGATNATTCATATTTNAGCGATTATAAGTCGTAGAGTTTGCAAANTGCAATCATTGAANAATTTCGTNGTAGNNNNNNNNTAACTTATAATNATAGTAAAANNNNNNNGTTTNAAAGAGGAAATTTGAATGAAAANAATTGTGATGTTACTAATTACGTACACNTACGCAATTATNATNAGGCAAAATGATGTGAAAGTGTANATAAAGACTTGTGTAGTTCACGATAATCACATCCTTAATGATTAATTAATAATGAGATCGAATCCCTTGACTTCACTCAAACACCTTCACGATGATGTCATCTGATGTAACAAAAGATATTAGTTAATNCTTACACGTTTNNATACANNNNNNNNNNNTATATGTGTATATATACGTGTAAAACATGAGATTCTCTTTTGCGATGTCTTCATCTATATAAATTCCACTTTTTTCCTTTAGNTGTNNNTCTTCNTTTTTCTANCGTANTCTATNTTTTTCCTTACTTTCNNGGNAAAAACTTTACGATCTTTCAATGTCAGAAGAATTTCAAGAATCNGAGGTTATNTTTTCCGATGAGTCTTTCACAANGAANGATAACAAGATCAGTCANAACNACGANAACTACGAAAGAAAGTCGACGGAGAAGGANAAAATTTCATCTCCGGTGANAATTCCGTCAAGAACTACTNTCCGGTATACGGAAGAGGAGGGAGAAATGANACCGCCACATGTCATAATCGAAAAACGAAGAACGGAGGCGCAAATGGCGTTTTCTTTTTGTACCCTTAAAGGAAGAGACTTGAGTCGACACCGTAACACCGTTCTTAGGATGACNGGNTTTTTGGAAGTNTANNATTGATCNCAAAATAAAATGAGAACCNGTCAAGTTTAGTTTGATCTATTTGNGTTNGGTTTACGTNGGTTTTCTTCNNNATTTGGATGNGATTTAGNTTTGAAACNAACCAATTANTGTATCATGTTTTACACGTATAACATAGCGTTGAAACGAACCAATGTATCACGGTTCTTATTNTTTNGTGAAGAAAAATTTAANATTGGGGACATTTTCNCATATTTTATTTGNTAAGATGTTGAATTTCCGGTTAACTTAATTGNAANTTTTTNTTTTAAATTATTGCTTGGTTTCTTGAGAAAANGAAAACCCATAANAATCAAATCTTATGATAAATTGAGTTATGGGGTTTGCTTGAGAAGTAATTGGTTTCTTCGCTTGTAAATTTNGTNNNNGTTTGGTCCACNTTNATGGGAGGAATNTGCTATTTGCCAATNTGCTTGCTAATGTGAGCTGTANAAAANCTTTATTTGCTATTGTGAACTGAACCAAGGGAGAGATACCTTGGAAAATTCGGNTAAACATTTGATAATGATACACATTGCTTAAACAAAACAAATTGATACCTTAAAAATTNGNTTGACTTCGGATTAGATANANGGTCCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.761840
PRIMER_LEFT_0_PENALTY=0.234379
PRIMER_RIGHT_0_PENALTY=0.527462
PRIMER_LEFT_0_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_0_SEQUENCE=CTCCGTCGACTTTCTTTCGT
PRIMER_LEFT_0=448,20
PRIMER_RIGHT_0=1282,20
PRIMER_LEFT_0_TM=60.234
PRIMER_RIGHT_0_TM=59.473
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=835
PRIMER_PAIR_1_PENALTY=1.032782
PRIMER_LEFT_1_PENALTY=0.234379
PRIMER_RIGHT_1_PENALTY=0.798404
PRIMER_LEFT_1_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_1_SEQUENCE=CCGTTCTTCGTTTTTCGATT
PRIMER_LEFT_1=448,20
PRIMER_RIGHT_1=1394,20
PRIMER_LEFT_1_TM=60.234
PRIMER_RIGHT_1_TM=59.202
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=947
PRIMER_PAIR_2_PENALTY=1.095420
PRIMER_LEFT_2_PENALTY=0.234379
PRIMER_RIGHT_2_PENALTY=0.861041
PRIMER_LEFT_2_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_2_SEQUENCE=ATTTCTCCCTCCTCTTCCGT
PRIMER_LEFT_2=448,20
PRIMER_RIGHT_2=1356,20
PRIMER_LEFT_2_TM=60.234
PRIMER_RIGHT_2_TM=59.139
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=909
PRIMER_PAIR_3_PENALTY=1.219923
PRIMER_LEFT_3_PENALTY=0.234379
PRIMER_RIGHT_3_PENALTY=0.985544
PRIMER_LEFT_3_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_3_SEQUENCE=TCCTTCTCCGTCGACTTTCT
PRIMER_LEFT_3=448,20
PRIMER_RIGHT_3=1287,20
PRIMER_LEFT_3_TM=60.234
PRIMER_RIGHT_3_TM=59.014
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=840
PRIMER_PAIR_4_PENALTY=1.333056
PRIMER_LEFT_4_PENALTY=0.234379
PRIMER_RIGHT_4_PENALTY=1.098677
PRIMER_LEFT_4_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_4_SEQUENCE=GCCTCCGTTCTTCGTTTTTC
PRIMER_LEFT_4=448,20
PRIMER_RIGHT_4=1398,20
PRIMER_LEFT_4_TM=60.234
PRIMER_RIGHT_4_TM=61.099
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=951
PRIMER_PAIR_5_PENALTY=1.362708
PRIMER_LEFT_5_PENALTY=0.234379
PRIMER_RIGHT_5_PENALTY=1.128329
PRIMER_LEFT_5_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_5_SEQUENCE=CCTTCTCCGTCGACTTTCTTT
PRIMER_LEFT_5=448,20
PRIMER_RIGHT_5=1286,21
PRIMER_LEFT_5_TM=60.234
PRIMER_RIGHT_5_TM=59.872
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=839
PRIMER_PAIR_6_PENALTY=1.421714
PRIMER_LEFT_6_PENALTY=0.560673
PRIMER_RIGHT_6_PENALTY=0.861041
PRIMER_LEFT_6_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_6_SEQUENCE=ATTTCTCCCTCCTCTTCCGT
PRIMER_LEFT_6=445,20
PRIMER_RIGHT_6=1356,20
PRIMER_LEFT_6_TM=59.439
PRIMER_RIGHT_6_TM=59.139
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=912
PRIMER_PAIR_7_PENALTY=1.546218
PRIMER_LEFT_7_PENALTY=0.560673
PRIMER_RIGHT_7_PENALTY=0.985544
PRIMER_LEFT_7_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_7_SEQUENCE=TCCTTCTCCGTCGACTTTCT
PRIMER_LEFT_7=445,20
PRIMER_RIGHT_7=1287,20
PRIMER_LEFT_7_TM=59.439
PRIMER_RIGHT_7_TM=59.014
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=843
PRIMER_PAIR_8_PENALTY=1.602094
PRIMER_LEFT_8_PENALTY=0.234379
PRIMER_RIGHT_8_PENALTY=1.367716
PRIMER_LEFT_8_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_8_SEQUENCE=TCCTTCTCCGTCGACTTTCTT
PRIMER_LEFT_8=448,20
PRIMER_RIGHT_8=1287,21
PRIMER_LEFT_8_TM=60.234
PRIMER_RIGHT_8_TM=60.368
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=840
PRIMER_PAIR_9_PENALTY=1.629528
PRIMER_LEFT_9_PENALTY=0.234379
PRIMER_RIGHT_9_PENALTY=1.395149
PRIMER_LEFT_9_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_9_SEQUENCE=GGGTACAAAAAGAAAACGCC
PRIMER_LEFT_9=448,20
PRIMER_RIGHT_9=1424,20
PRIMER_LEFT_9_TM=60.234
PRIMER_RIGHT_9_TM=58.605
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=11.1000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=977
PRIMER_PAIR_10_PENALTY=1.659350
PRIMER_LEFT_10_PENALTY=0.560673
PRIMER_RIGHT_10_PENALTY=1.098677
PRIMER_LEFT_10_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_10_SEQUENCE=GCCTCCGTTCTTCGTTTTTC
PRIMER_LEFT_10=445,20
PRIMER_RIGHT_10=1398,20
PRIMER_LEFT_10_TM=59.439
PRIMER_RIGHT_10_TM=61.099
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=954
PRIMER_PAIR_11_PENALTY=1.689002
PRIMER_LEFT_11_PENALTY=0.560673
PRIMER_RIGHT_11_PENALTY=1.128329
PRIMER_LEFT_11_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_11_SEQUENCE=CCTTCTCCGTCGACTTTCTTT
PRIMER_LEFT_11=445,20
PRIMER_RIGHT_11=1286,21
PRIMER_LEFT_11_TM=59.439
PRIMER_RIGHT_11_TM=59.872
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=842
PRIMER_PAIR_12_PENALTY=1.703138
PRIMER_LEFT_12_PENALTY=0.234379
PRIMER_RIGHT_12_PENALTY=1.468760
PRIMER_LEFT_12_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_12_SEQUENCE=AGGGTACAAAAAGAAAACGCC
PRIMER_LEFT_12=448,20
PRIMER_RIGHT_12=1425,21
PRIMER_LEFT_12_TM=60.234
PRIMER_RIGHT_12_TM=59.531
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=11.1000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=978
PRIMER_PAIR_13_PENALTY=1.710695
PRIMER_LEFT_13_PENALTY=0.234379
PRIMER_RIGHT_13_PENALTY=1.476317
PRIMER_LEFT_13_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_13_SEQUENCE=CCTTCTCCGTCGACTTTCTT
PRIMER_LEFT_13=448,20
PRIMER_RIGHT_13=1286,20
PRIMER_LEFT_13_TM=60.234
PRIMER_RIGHT_13_TM=58.524
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=839
PRIMER_PAIR_14_PENALTY=1.731534
PRIMER_LEFT_14_PENALTY=0.234379
PRIMER_RIGHT_14_PENALTY=1.497155
PRIMER_LEFT_14_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_14_SEQUENCE=TCCGTCGACTTTCTTTCGTAG
PRIMER_LEFT_14=448,20
PRIMER_RIGHT_14=1281,21
PRIMER_LEFT_14_TM=60.234
PRIMER_RIGHT_14_TM=59.503
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=7.4000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=834
PRIMER_PAIR_15_PENALTY=1.955822
PRIMER_LEFT_15_PENALTY=0.560673
PRIMER_RIGHT_15_PENALTY=1.395149
PRIMER_LEFT_15_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_15_SEQUENCE=GGGTACAAAAAGAAAACGCC
PRIMER_LEFT_15=445,20
PRIMER_RIGHT_15=1424,20
PRIMER_LEFT_15_TM=59.439
PRIMER_RIGHT_15_TM=58.605
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=11.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=980
PRIMER_PAIR_16_PENALTY=1.992155
PRIMER_LEFT_16_PENALTY=0.234379
PRIMER_RIGHT_16_PENALTY=1.757776
PRIMER_LEFT_16_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_16_SEQUENCE=TCATTTCTCCCTCCTCTTCC
PRIMER_LEFT_16=448,20
PRIMER_RIGHT_16=1358,20
PRIMER_LEFT_16_TM=60.234
PRIMER_RIGHT_16_TM=58.242
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=911
PRIMER_PAIR_17_PENALTY=2.029433
PRIMER_LEFT_17_PENALTY=0.560673
PRIMER_RIGHT_17_PENALTY=1.468760
PRIMER_LEFT_17_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_17_SEQUENCE=AGGGTACAAAAAGAAAACGCC
PRIMER_LEFT_17=445,20
PRIMER_RIGHT_17=1425,21
PRIMER_LEFT_17_TM=59.439
PRIMER_RIGHT_17_TM=59.531
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=11.1000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=981
PRIMER_PAIR_18_PENALTY=2.057829
PRIMER_LEFT_18_PENALTY=0.560673
PRIMER_RIGHT_18_PENALTY=1.497155
PRIMER_LEFT_18_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_18_SEQUENCE=TCCGTCGACTTTCTTTCGTAG
PRIMER_LEFT_18=445,20
PRIMER_RIGHT_18=1281,21
PRIMER_LEFT_18_TM=59.439
PRIMER_RIGHT_18_TM=59.503
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.4000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=837
PRIMER_PAIR_19_PENALTY=2.113787
PRIMER_LEFT_19_PENALTY=1.252746
PRIMER_RIGHT_19_PENALTY=0.861041
PRIMER_LEFT_19_SEQUENCE=GTCCGAAAGCAACAAATCAA
PRIMER_RIGHT_19_SEQUENCE=ATTTCTCCCTCCTCTTCCGT
PRIMER_LEFT_19=449,20
PRIMER_RIGHT_19=1356,20
PRIMER_LEFT_19_TM=58.747
PRIMER_RIGHT_19_TM=59.139
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=908
PRIMER_PAIR_20_PENALTY=2.113787
PRIMER_LEFT_20_PENALTY=1.252746
PRIMER_RIGHT_20_PENALTY=0.861041
PRIMER_LEFT_20_SEQUENCE=TTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_20_SEQUENCE=ATTTCTCCCTCCTCTTCCGT
PRIMER_LEFT_20=447,20
PRIMER_RIGHT_20=1356,20
PRIMER_LEFT_20_TM=58.747
PRIMER_RIGHT_20_TM=59.139
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=9.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=910
PRIMER_PAIR_21_PENALTY=2.122740
PRIMER_LEFT_21_PENALTY=1.261699
PRIMER_RIGHT_21_PENALTY=0.861041
PRIMER_LEFT_21_SEQUENCE=CTTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_21_SEQUENCE=ATTTCTCCCTCCTCTTCCGT
PRIMER_LEFT_21=446,21
PRIMER_RIGHT_21=1356,20
PRIMER_LEFT_21_TM=59.738
PRIMER_RIGHT_21_TM=59.139
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=911
PRIMER_PAIR_22_PENALTY=2.238291
PRIMER_LEFT_22_PENALTY=1.252746
PRIMER_RIGHT_22_PENALTY=0.985544
PRIMER_LEFT_22_SEQUENCE=GTCCGAAAGCAACAAATCAA
PRIMER_RIGHT_22_SEQUENCE=TCCTTCTCCGTCGACTTTCT
PRIMER_LEFT_22=449,20
PRIMER_RIGHT_22=1287,20
PRIMER_LEFT_22_TM=58.747
PRIMER_RIGHT_22_TM=59.014
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.9000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=839
PRIMER_PAIR_23_PENALTY=2.238291
PRIMER_LEFT_23_PENALTY=1.252746
PRIMER_RIGHT_23_PENALTY=0.985544
PRIMER_LEFT_23_SEQUENCE=TTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_23_SEQUENCE=TCCTTCTCCGTCGACTTTCT
PRIMER_LEFT_23=447,20
PRIMER_RIGHT_23=1287,20
PRIMER_LEFT_23_TM=58.747
PRIMER_RIGHT_23_TM=59.014
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.9000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=841
PRIMER_PAIR_24_PENALTY=2.247244
PRIMER_LEFT_24_PENALTY=1.261699
PRIMER_RIGHT_24_PENALTY=0.985544
PRIMER_LEFT_24_SEQUENCE=CTTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_24_SEQUENCE=TCCTTCTCCGTCGACTTTCT
PRIMER_LEFT_24=446,21
PRIMER_RIGHT_24=1287,20
PRIMER_LEFT_24_TM=59.738
PRIMER_RIGHT_24_TM=59.014
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=842
PRIMER_PAIR_25_PENALTY=2.286300
PRIMER_LEFT_25_PENALTY=0.560673
PRIMER_RIGHT_25_PENALTY=1.725626
PRIMER_LEFT_25_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_25_SEQUENCE=CGATTATGACATGTGGCGGT
PRIMER_LEFT_25=445,20
PRIMER_RIGHT_25=1379,20
PRIMER_LEFT_25_TM=59.439
PRIMER_RIGHT_25_TM=61.726
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=11.1000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=935
PRIMER_PAIR_26_PENALTY=2.318450
PRIMER_LEFT_26_PENALTY=0.560673
PRIMER_RIGHT_26_PENALTY=1.757776
PRIMER_LEFT_26_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_26_SEQUENCE=TCATTTCTCCCTCCTCTTCC
PRIMER_LEFT_26=445,20
PRIMER_RIGHT_26=1358,20
PRIMER_LEFT_26_TM=59.439
PRIMER_RIGHT_26_TM=58.242
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=914
PRIMER_PAIR_27_PENALTY=2.351423
PRIMER_LEFT_27_PENALTY=1.252746
PRIMER_RIGHT_27_PENALTY=1.098677
PRIMER_LEFT_27_SEQUENCE=GTCCGAAAGCAACAAATCAA
PRIMER_RIGHT_27_SEQUENCE=GCCTCCGTTCTTCGTTTTTC
PRIMER_LEFT_27=449,20
PRIMER_RIGHT_27=1398,20
PRIMER_LEFT_27_TM=58.747
PRIMER_RIGHT_27_TM=61.099
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.3000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=950
PRIMER_PAIR_28_PENALTY=2.351423
PRIMER_LEFT_28_PENALTY=1.252746
PRIMER_RIGHT_28_PENALTY=1.098677
PRIMER_LEFT_28_SEQUENCE=TTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_28_SEQUENCE=GCCTCCGTTCTTCGTTTTTC
PRIMER_LEFT_28=447,20
PRIMER_RIGHT_28=1398,20
PRIMER_LEFT_28_TM=58.747
PRIMER_RIGHT_28_TM=61.099
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=952
PRIMER_PAIR_29_PENALTY=2.360376
PRIMER_LEFT_29_PENALTY=1.261699
PRIMER_RIGHT_29_PENALTY=1.098677
PRIMER_LEFT_29_SEQUENCE=CTTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_29_SEQUENCE=GCCTCCGTTCTTCGTTTTTC
PRIMER_LEFT_29=446,21
PRIMER_RIGHT_29=1398,20
PRIMER_LEFT_29_TM=59.738
PRIMER_RIGHT_29_TM=61.099
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=953
PRIMER_PAIR_30_PENALTY=2.381075
PRIMER_LEFT_30_PENALTY=1.252746
PRIMER_RIGHT_30_PENALTY=1.128329
PRIMER_LEFT_30_SEQUENCE=GTCCGAAAGCAACAAATCAA
PRIMER_RIGHT_30_SEQUENCE=CCTTCTCCGTCGACTTTCTTT
PRIMER_LEFT_30=449,20
PRIMER_RIGHT_30=1286,21
PRIMER_LEFT_30_TM=58.747
PRIMER_RIGHT_30_TM=59.872
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.9000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=838
PRIMER_PAIR_31_PENALTY=2.381075
PRIMER_LEFT_31_PENALTY=1.252746
PRIMER_RIGHT_31_PENALTY=1.128329
PRIMER_LEFT_31_SEQUENCE=TTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_31_SEQUENCE=CCTTCTCCGTCGACTTTCTTT
PRIMER_LEFT_31=447,20
PRIMER_RIGHT_31=1286,21
PRIMER_LEFT_31_TM=58.747
PRIMER_RIGHT_31_TM=59.872
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=840
PRIMER_PAIR_32_PENALTY=2.390028
PRIMER_LEFT_32_PENALTY=1.261699
PRIMER_RIGHT_32_PENALTY=1.128329
PRIMER_LEFT_32_SEQUENCE=CTTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_32_SEQUENCE=CCTTCTCCGTCGACTTTCTTT
PRIMER_LEFT_32=446,21
PRIMER_RIGHT_32=1286,21
PRIMER_LEFT_32_TM=59.738
PRIMER_RIGHT_32_TM=59.872
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=841
PRIMER_PAIR_33_PENALTY=2.414819
PRIMER_LEFT_33_PENALTY=0.234379
PRIMER_RIGHT_33_PENALTY=2.180440
PRIMER_LEFT_33_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_33_SEQUENCE=CCGTCGACTTTCTTTCGTAGTT
PRIMER_LEFT_33=448,20
PRIMER_RIGHT_33=1280,22
PRIMER_LEFT_33_TM=60.234
PRIMER_RIGHT_33_TM=59.820
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=7.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=833
PRIMER_PAIR_34_PENALTY=2.424725
PRIMER_LEFT_34_PENALTY=0.234379
PRIMER_RIGHT_34_PENALTY=2.190347
PRIMER_LEFT_34_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_34_SEQUENCE=GGTACAAAAAGAAAACGCCA
PRIMER_LEFT_34=448,20
PRIMER_RIGHT_34=1423,20
PRIMER_LEFT_34_TM=60.234
PRIMER_RIGHT_34_TM=57.810
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=11.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=976
PRIMER_PAIR_35_PENALTY=2.436193
PRIMER_LEFT_35_PENALTY=0.234379
PRIMER_RIGHT_35_PENALTY=2.201814
PRIMER_LEFT_35_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_35_SEQUENCE=GGGTACAAAAAGAAAACGCCA
PRIMER_LEFT_35=448,20
PRIMER_RIGHT_35=1424,21
PRIMER_LEFT_35_TM=60.234
PRIMER_RIGHT_35_TM=61.202
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=11.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=977
PRIMER_PAIR_36_PENALTY=2.529353
PRIMER_LEFT_36_PENALTY=0.234379
PRIMER_RIGHT_36_PENALTY=2.294975
PRIMER_LEFT_36_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_36_SEQUENCE=TCCGTCGACTTTCTTTCGTAGT
PRIMER_LEFT_36=448,20
PRIMER_RIGHT_36=1281,22
PRIMER_LEFT_36_TM=60.234
PRIMER_RIGHT_36_TM=60.295
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=5.1000
PRIMER_PAIR_36_COMPL_ANY=7.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=834
PRIMER_PAIR_37_PENALTY=2.531835
PRIMER_LEFT_37_PENALTY=0.234379
PRIMER_RIGHT_37_PENALTY=2.297456
PRIMER_LEFT_37_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_37_SEQUENCE=TCTCCGTCGACTTTCTTTCGT
PRIMER_LEFT_37=448,20
PRIMER_RIGHT_37=1283,21
PRIMER_LEFT_37_TM=60.234
PRIMER_RIGHT_37_TM=61.297
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=7.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=836
PRIMER_PAIR_38_PENALTY=2.597584
PRIMER_LEFT_38_PENALTY=0.234379
PRIMER_RIGHT_38_PENALTY=2.363205
PRIMER_LEFT_38_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_38_SEQUENCE=CCGTCGACTTTCTTTCGTAG
PRIMER_LEFT_38=448,20
PRIMER_RIGHT_38=1280,20
PRIMER_LEFT_38_TM=60.234
PRIMER_RIGHT_38_TM=57.637
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.4000
PRIMER_PAIR_38_COMPL_ANY=7.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=833
PRIMER_PAIR_39_PENALTY=2.647895
PRIMER_LEFT_39_PENALTY=1.252746
PRIMER_RIGHT_39_PENALTY=1.395149
PRIMER_LEFT_39_SEQUENCE=GTCCGAAAGCAACAAATCAA
PRIMER_RIGHT_39_SEQUENCE=GGGTACAAAAAGAAAACGCC
PRIMER_LEFT_39=449,20
PRIMER_RIGHT_39=1424,20
PRIMER_LEFT_39_TM=58.747
PRIMER_RIGHT_39_TM=58.605
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=11.1000
PRIMER_PAIR_39_COMPL_ANY=2.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=976
PRIMER_PAIR_40_PENALTY=2.647895
PRIMER_LEFT_40_PENALTY=1.252746
PRIMER_RIGHT_40_PENALTY=1.395149
PRIMER_LEFT_40_SEQUENCE=TTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_40_SEQUENCE=GGGTACAAAAAGAAAACGCC
PRIMER_LEFT_40=447,20
PRIMER_RIGHT_40=1424,20
PRIMER_LEFT_40_TM=58.747
PRIMER_RIGHT_40_TM=58.605
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=11.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=978
PRIMER_PAIR_41_PENALTY=2.650745
PRIMER_LEFT_41_PENALTY=0.234379
PRIMER_RIGHT_41_PENALTY=2.416367
PRIMER_LEFT_41_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_41_SEQUENCE=CTCCGTCGACTTTCTTTCGTAG
PRIMER_LEFT_41=448,20
PRIMER_RIGHT_41=1282,22
PRIMER_LEFT_41_TM=60.234
PRIMER_RIGHT_41_TM=60.416
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=7.4000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=835
PRIMER_PAIR_42_PENALTY=2.656848
PRIMER_LEFT_42_PENALTY=1.261699
PRIMER_RIGHT_42_PENALTY=1.395149
PRIMER_LEFT_42_SEQUENCE=CTTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_42_SEQUENCE=GGGTACAAAAAGAAAACGCC
PRIMER_LEFT_42=446,21
PRIMER_RIGHT_42=1424,20
PRIMER_LEFT_42_TM=59.738
PRIMER_RIGHT_42_TM=58.605
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=11.1000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=979
PRIMER_PAIR_43_PENALTY=2.711096
PRIMER_LEFT_43_PENALTY=0.234379
PRIMER_RIGHT_43_PENALTY=2.476717
PRIMER_LEFT_43_SEQUENCE=TGTCCGAAAGCAACAAATCA
PRIMER_RIGHT_43_SEQUENCE=CCGTCGACTTTCTTTCGTAGT
PRIMER_LEFT_43=448,20
PRIMER_RIGHT_43=1280,21
PRIMER_LEFT_43_TM=60.234
PRIMER_RIGHT_43_TM=58.523
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.9000
PRIMER_RIGHT_43_END_STABILITY=5.1000
PRIMER_PAIR_43_COMPL_ANY=7.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=833
PRIMER_PAIR_44_PENALTY=2.721506
PRIMER_LEFT_44_PENALTY=1.252746
PRIMER_RIGHT_44_PENALTY=1.468760
PRIMER_LEFT_44_SEQUENCE=GTCCGAAAGCAACAAATCAA
PRIMER_RIGHT_44_SEQUENCE=AGGGTACAAAAAGAAAACGCC
PRIMER_LEFT_44=449,20
PRIMER_RIGHT_44=1425,21
PRIMER_LEFT_44_TM=58.747
PRIMER_RIGHT_44_TM=59.531
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=11.1000
PRIMER_PAIR_44_COMPL_ANY=2.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=977
PRIMER_PAIR_45_PENALTY=2.721506
PRIMER_LEFT_45_PENALTY=1.252746
PRIMER_RIGHT_45_PENALTY=1.468760
PRIMER_LEFT_45_SEQUENCE=TTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_45_SEQUENCE=AGGGTACAAAAAGAAAACGCC
PRIMER_LEFT_45=447,20
PRIMER_RIGHT_45=1425,21
PRIMER_LEFT_45_TM=58.747
PRIMER_RIGHT_45_TM=59.531
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=11.1000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=979
PRIMER_PAIR_46_PENALTY=2.730459
PRIMER_LEFT_46_PENALTY=1.261699
PRIMER_RIGHT_46_PENALTY=1.468760
PRIMER_LEFT_46_SEQUENCE=CTTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_46_SEQUENCE=AGGGTACAAAAAGAAAACGCC
PRIMER_LEFT_46=446,21
PRIMER_RIGHT_46=1425,21
PRIMER_LEFT_46_TM=59.738
PRIMER_RIGHT_46_TM=59.531
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=11.1000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=980
PRIMER_PAIR_47_PENALTY=2.741114
PRIMER_LEFT_47_PENALTY=0.560673
PRIMER_RIGHT_47_PENALTY=2.180440
PRIMER_LEFT_47_SEQUENCE=TCTTGTCCGAAAGCAACAAA
PRIMER_RIGHT_47_SEQUENCE=CCGTCGACTTTCTTTCGTAGTT
PRIMER_LEFT_47=445,20
PRIMER_RIGHT_47=1280,22
PRIMER_LEFT_47_TM=59.439
PRIMER_RIGHT_47_TM=59.820
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=5.7000
PRIMER_PAIR_47_COMPL_ANY=7.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=836
PRIMER_PAIR_48_PENALTY=2.749902
PRIMER_LEFT_48_PENALTY=1.252746
PRIMER_RIGHT_48_PENALTY=1.497155
PRIMER_LEFT_48_SEQUENCE=GTCCGAAAGCAACAAATCAA
PRIMER_RIGHT_48_SEQUENCE=TCCGTCGACTTTCTTTCGTAG
PRIMER_LEFT_48=449,20
PRIMER_RIGHT_48=1281,21
PRIMER_LEFT_48_TM=58.747
PRIMER_RIGHT_48_TM=59.503
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=7.4000
PRIMER_PAIR_48_COMPL_ANY=7.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=833
PRIMER_PAIR_49_PENALTY=2.749902
PRIMER_LEFT_49_PENALTY=1.252746
PRIMER_RIGHT_49_PENALTY=1.497155
PRIMER_LEFT_49_SEQUENCE=TTGTCCGAAAGCAACAAATC
PRIMER_RIGHT_49_SEQUENCE=TCCGTCGACTTTCTTTCGTAG
PRIMER_LEFT_49=447,20
PRIMER_RIGHT_49=1281,21
PRIMER_LEFT_49_TM=58.747
PRIMER_RIGHT_49_TM=59.503
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=7.4000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=835
=
SEQUENCE_ID=MAGIC.446_4_4110260_4110554_wu-0_oy-0_1293942_17673_TRUE
SEQUENCE_TARGET=950,394
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATNCTNNAGTGTGTGAATCCGTCTTCCACATNTTGGCGCCGTCTGTNGGGANCGGAGGATTCTTGAGTCGTCAGATTTCTTCNGTGTATTACATACCCNAAGTNAGNCGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_4492390_4492450_can-0_ler-0_54993_855416_TRUE
SEQUENCE_TARGET=950,160
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_5665127_5665838_hi-0_no-0_5504868_1400_TRUE
SEQUENCE_TARGET=950,811
SEQUENCE_TEMPLATE=TAAAAAAGGATTAGCAGAAGAGAGATCAGAGAGATGCCACGTGGAGCCTACGGCCAATGGGAGAGCGATAAGCTTATCAAAGATTTCAAAGTGCGGAGGGTAAAGTGGGCGACGTTGCTTTTCCACGTGNCAATNTNTCATTGGTANGAAANGTTAAAAGTTAGATTTCCGCTTAGTCGGTATTTNGGTCCCATACTGTCNTCGCAACTTGGNCCTCTCTCTTGCGNTCAGAAGGTTTTGTCTTTTNAGTTTCTTCTTGATTTTGNTTTTATCCTTTGTTATTTTTAGTCTGGTCCGTNTTTTCTCCGTATCTTGTTTCAAATACATTNTCTCGTAAAGAAACCGCNTTTTTTGGTTTAGTACTGAATGATAAAAGTATTGTTTTCNTACGAAATTTANGTCAACCGNCCTAGTNCTAGAGGATCACCTAGACCATATGCATGGGACTTNGTCGAGCTTCGATGTGGATTAGCCCNAGTTATCAATAGAATACTGAAAATTGGCNNNNNTAACTTTGATTTAATGGAAATGCNTTTTTATANNATACTNAAAATTTAANAGANATGATGATNGATGAGTAGTAAATTTCAAATGGTATAACAAGTTTAATTGTTTTTTTAGTAAGTTATATATACTGANCAAGACGTTTTTGTTTGAGTCAGTGACTATCNCTCTTTGTTTTTACTCAACTTAATTCCATANATCCTGGTACTTATAACGTGTACAATCTAGAATCACAAGCGTTTAGAGATAGTGATAATTAGCTTGGCTACTTTCTCAGCTGTGTTGTTGCTTGATCGANGNNGGAAAATGANAGTGGNCATATTGAAGTTCTTGCTTAGNGCTATTGNTAATAGAACGGTTNGAGAGCCGGCCAAGCTTATGGATAATATATTTATANATGGAGAATGAGAATAACATAAAGATGAGANATNGTTGAACCTAATTAATNAAAAAAACAATTTACATGAANTTTTTTTCTTTANAAGATTTCANTCTGNAACATGCATAGAAACTTGTCTCATNACCCTCGTAGTCNTATAGCAAGCATTAGTTTTATGATAGAGATGTTTCNGTTACGAAAGAAANTTTAAACCAACTTAAGTGGTTGTAAATCACGTTGTANTTTTTATGTTTATGTACAANTNCAAATTACAAATCTTCNTTTTTAATTATAATCTTTGCCACTGAAATTCACGATAATATCACTAAGGTTCGATGCAATTCGTCAAACATNTCACGTACTAAAATTTGTTNGGCGANNGANGGAGACATAGATGTAAATGGAAACGTNNACTCGTAAATTGTGTCATCTTTCGTGGCCCATNAANTNGAGATGGAGTTTTATCGTTTTCTATATCNNNNNNNNNNNNNNNNNNNNNNAANAAGGTTTTATTATAAAAAGTACCAAAGAAATGGACCGTAGTCACCATNNNNNNNNTTACATGGTTACATGGCGCGACACGTAGTGTCACTTAATGCTGCAAGTGTTTTTTCCTCTTTGTTTTTTGCCTCCCTGAATATTTTAAATTTGNTATATAAAATNTTTCTGCTGACGAGTTTTAGTTACTCTAGTTTCTAAAATGGTCTAGAAAAAGTTGTGNTATATAANTATAATAACTCGGTAANGGCTACGTACAGAGTCAGCCACGTAACANTAAATGGATGTGTCATGGACCCGTATTACACTCNNCATTATACACGNNTNNNGNNTANATNNNNTANNNNNNGATNANNNANNTTTNNNNTNTCAANNNNGANANANNTANTNNNGTANNNNNTNTTCNAAANNCATAGTNANTNTANNTNATNANAAATANGTNTTNANTNGTTTNNAAACNANTNNNCANNNNNNNANTTATTNTTNTNNCNNNNANTNANTNTGANTNNTTTNNNANNNGNAANTANCTATNTNNNNNNTGNNNNNNNAANNNNNNTTNTTTNNTNAANANTANNTNTNATTNTNNANNNTACANNNANNCCACAACTAGAAAGACGANTCCAAAAACNAAAGNAAATAGGAANTACTCGACCTTTNTTTTCTAACNTCAATCCACCACATNATATTTGTNGTTTTTAGCGNAATAATTNAGTTCTAACACACCACATGATTTGCTAAGCTACCAGTACTACTTTCTTTGTTAGGCCNTGAGAAATTGAATATTCCTTCCTCTTATTCTGCATATTCTTTGTATTTGTTTTGTTACCTACCTCTTCTAGTGTGTAGCCTACGTAAATATACACTCAAGTTGTTTTGATGTAACCNGTTTTCTCCACCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACACTACATATCAATTCNTACTTATTATAANTACACTNATTANATNTNAATGCATGAANNNAAACTCGAATTCGATATNNTNCGATTCGTCAAAAAANNNNNNNNNNNNNNNNNNTGGTATCAATTAGAGGTCTGAATTTGTTCAATATCGTCCACAATTCCCTACCTAAAAAATTAGAGCTTTTTGGACCAAATCCAAAAATGGATCTAAAAAGTGAAAACCAAAATATTCCTTTAAGAATCTACACAGATTCGAACCAAACCGGATTAAACCGNATAGTCNACGGCACGTGTGGAAACGCGNNNCGTTTCAAAAACATTTGGGGTANTNNNNNNNNNNNNNNNNNNNNNNNNATCAGGGTTTTAGGAGAAACCCAGGAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_7199563_7199568_ws-0_po-0_6389_93910_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=CTTGATNTTGCTCAAATGCATCATCTTTGGCATCTCNGTGATGGNTGTATCATCCATAAGTAAAATCTCCAANGATTCCATGTCTTCCTTAATTTCTGGAAAAANCTCTAGTTGTGANCAACCTGAGAGTATTAGCTCTTGAAGACATTTCAGCTTATANAGATCAGANNAAAGATGCTTCAGCTTTTTGCAGTTCTTCAGATTCAGCAAAGCAAGTCTTCTNAAGGTTTNAATGGATTNGGGAAGGCTCTNTATANNAGTGCCATCTAAAAGTAGAACTTCAACATTTTCCGAGATNANTGGGAATTNCTNTANGCTNGAGCAGCCANTGAGAATAAGAGTTTGCAAGGATTGNGTTTNGNNNCCTTNTGGAAGACTCNNAAGGNTTGTGCAATCCCTGAGATTGAGGTANATAAGCTNCTCCNAACNATTNATNNNNNNCGGCAATTNTTTNAAACTNGTACATCCTTCNAGATTNAACCTCTCAAGATTNTGAGCATTAGCCAAACCCANACACTGACGTAAATTNANNGANNGACTAAGGTCNACCCATTTGAGCATTCCANCATCCTGTNAAANAAAANNCAACCACAAGTCNAATTTTNGATGCAAATNANNATTCACTTTAGCTTNGGACTNATCANACNGACNTAAANCATACCTTTTNNTNATCCCAAANTTNTNNTAATTGACTATGTGGCAATTTAAGGTCNACAAGNTTCTTNGGGTCAAAGTCTANAGGGATACNCTGTAGTGGNTAACCATGCCANTGTAGATATGTAAGCTCNTTAGGAAGGNNNNNNNNNNNNNTCNGCAAGTGNAGTTTAAANTCTGCTTNNCATCCACGGGAACAATGAGANNNATAAANTTTCAAGTATTTGAGATTNTACATCCNNNNNNNNNNNNNNNNNCTTAGACNCATTGCTCTTANTTTNGATGTGTCGAGNAAAATNCCTCTTATCTTNTCAGTTCCCTNTCAATCAAGNNGTANATGTACANTNNTTANNTCNAAGCANGATNNTNNANNATAATACNNAATTGTAGANAATNNNATATCANNANGANATGNNNNNNNTCATTACCAGGCCTNNGGTTAGCANGTCACNAATATCNTCACTGTCCCATANTCTGATATGCCATTGGNACTGGTTACCATGTCGTGANAGCCACNTANAGTCTCTTATACCTATNGTTTCTNCTTTTANACTAATCTCCTTGGCCATNGTNTGCAACATNTCATGCATCTCGATGCGATTATCAGAAAGAGTGATCAGACATTTGTCAACGAGATCCTTTANNACGCCAGAGACGTCCNCACCATGACTGNTGAGAAGGCTTGTCACGTAATCCACATTCTCTGATCTGAAGAAACATGCAATATCCAGAAAAACATTCTTCTGNTCANTGGTAAGCTCCTCATAGCTTGTTTCNAGAACTTCGTAGATATCTCCATGTGATCTNCTTTTCANTCTATCCANTTTAGCTTCCCAGTACAGATNATCCCTCTCANAAAGATCAGATCCTAACACCTTNAGAGCCAAAGGGTGGCCTTTAGCGTAATCAAGAACCATATTTGTCAAACCTTCGAACTCTTTCAAAGGNAAAGAGTNACTAAAAGCATTCAAGCTAAAGAGCTTNAAGGCTTCTCTATCNTTCANTTTCGGGAGCACNTATTNTCGGCCTTTGATCGTCTCNATCAGTTTACNNTCTCTAGTAGTAATGATAATTCTACTTCCTCCTTGANACCATTTGCANTGNCCCATNAGATANNTGATNTGCTTTTCATCATTCACATCATCAAGCACAATAAGNANCCTCTTNCTCTTAANCCGCNGCTCAAATCTCTCATGTGCATTTCCAGGAGCTCCAATCTCCAAATCTCNATCATTNAGTACNGTNNAAAAGAGTTTCNGCAGCAAAGATTCTAACCCGCTTCTACCTGAATTTTCNCGGATATTNGTAAGNAAGCAGCTACCGTNNAACTGACCNCGCATNCGTCCATACA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.971763
PRIMER_LEFT_0_PENALTY=1.876134
PRIMER_RIGHT_0_PENALTY=0.095629
PRIMER_LEFT_0_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_0_SEQUENCE=TGATTACGCTAAAGGCCACC
PRIMER_LEFT_0=839,20
PRIMER_RIGHT_0=1562,20
PRIMER_LEFT_0_TM=61.876
PRIMER_RIGHT_0_TM=60.096
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=724
PRIMER_PAIR_1_PENALTY=2.304615
PRIMER_LEFT_1_PENALTY=1.876134
PRIMER_RIGHT_1_PENALTY=0.428481
PRIMER_LEFT_1_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_1_SEQUENCE=TGTGGATTACGTGACAAGCC
PRIMER_LEFT_1=839,20
PRIMER_RIGHT_1=1352,20
PRIMER_LEFT_1_TM=61.876
PRIMER_RIGHT_1_TM=59.572
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=514
PRIMER_PAIR_2_PENALTY=2.697153
PRIMER_LEFT_2_PENALTY=1.876134
PRIMER_RIGHT_2_PENALTY=0.821019
PRIMER_LEFT_2_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_2_SEQUENCE=GATTACGCTAAAGGCCACCC
PRIMER_LEFT_2=839,20
PRIMER_RIGHT_2=1561,20
PRIMER_LEFT_2_TM=61.876
PRIMER_RIGHT_2_TM=60.821
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=723
PRIMER_PAIR_3_PENALTY=3.002327
PRIMER_LEFT_3_PENALTY=1.876134
PRIMER_RIGHT_3_PENALTY=1.126193
PRIMER_LEFT_3_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_3_SEQUENCE=ATGTGGATTACGTGACAAGCC
PRIMER_LEFT_3=839,20
PRIMER_RIGHT_3=1353,21
PRIMER_LEFT_3_TM=61.876
PRIMER_RIGHT_3_TM=59.874
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=515
PRIMER_PAIR_4_PENALTY=3.949833
PRIMER_LEFT_4_PENALTY=1.876134
PRIMER_RIGHT_4_PENALTY=2.073699
PRIMER_LEFT_4_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_4_SEQUENCE=TTGATTACGCTAAAGGCCAC
PRIMER_LEFT_4=839,20
PRIMER_RIGHT_4=1563,20
PRIMER_LEFT_4_TM=61.876
PRIMER_RIGHT_4_TM=57.926
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=725
PRIMER_PAIR_5_PENALTY=3.964610
PRIMER_LEFT_5_PENALTY=1.876134
PRIMER_RIGHT_5_PENALTY=2.088476
PRIMER_LEFT_5_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_5_SEQUENCE=CTTGATTACGCTAAAGGCCAC
PRIMER_LEFT_5=839,20
PRIMER_RIGHT_5=1564,21
PRIMER_LEFT_5_TM=61.876
PRIMER_RIGHT_5_TM=58.912
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=726
PRIMER_PAIR_6_PENALTY=4.195202
PRIMER_LEFT_6_PENALTY=1.876134
PRIMER_RIGHT_6_PENALTY=2.319068
PRIMER_LEFT_6_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_6_SEQUENCE=TTGATTACGCTAAAGGCCACC
PRIMER_LEFT_6=839,20
PRIMER_RIGHT_6=1563,21
PRIMER_LEFT_6_TM=61.876
PRIMER_RIGHT_6_TM=61.319
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=725
PRIMER_PAIR_7_PENALTY=4.490441
PRIMER_LEFT_7_PENALTY=1.876134
PRIMER_RIGHT_7_PENALTY=2.614307
PRIMER_LEFT_7_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_7_SEQUENCE=TCTTGATTACGCTAAAGGCCAC
PRIMER_LEFT_7=839,20
PRIMER_RIGHT_7=1565,22
PRIMER_LEFT_7_TM=61.876
PRIMER_RIGHT_7_TM=60.614
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=727
PRIMER_PAIR_8_PENALTY=4.545097
PRIMER_LEFT_8_PENALTY=1.876134
PRIMER_RIGHT_8_PENALTY=2.668963
PRIMER_LEFT_8_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_8_SEQUENCE=CTAAAGGCCACCCTTTGGCT
PRIMER_LEFT_8=839,20
PRIMER_RIGHT_8=1554,20
PRIMER_LEFT_8_TM=61.876
PRIMER_RIGHT_8_TM=62.669
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=716
PRIMER_PAIR_9_PENALTY=4.871005
PRIMER_LEFT_9_PENALTY=1.876134
PRIMER_RIGHT_9_PENALTY=2.994871
PRIMER_LEFT_9_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_9_SEQUENCE=GTGCTTGATGATGTGAATGATG
PRIMER_LEFT_9=839,20
PRIMER_RIGHT_9=1804,22
PRIMER_LEFT_9_TM=61.876
PRIMER_RIGHT_9_TM=59.005
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=966
PRIMER_PAIR_10_PENALTY=5.030705
PRIMER_LEFT_10_PENALTY=1.876134
PRIMER_RIGHT_10_PENALTY=3.154571
PRIMER_LEFT_10_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_10_SEQUENCE=AATGTGGATTACGTGACAAGCC
PRIMER_LEFT_10=839,20
PRIMER_RIGHT_10=1354,22
PRIMER_LEFT_10_TM=61.876
PRIMER_RIGHT_10_TM=61.155
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.455
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=516
PRIMER_PAIR_11_PENALTY=5.419881
PRIMER_LEFT_11_PENALTY=1.876134
PRIMER_RIGHT_11_PENALTY=3.543747
PRIMER_LEFT_11_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_11_SEQUENCE=TGGTTCTTGATTACGCTAAAGG
PRIMER_LEFT_11=839,20
PRIMER_RIGHT_11=1569,22
PRIMER_LEFT_11_TM=61.876
PRIMER_RIGHT_11_TM=58.456
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=731
PRIMER_PAIR_12_PENALTY=5.424473
PRIMER_LEFT_12_PENALTY=1.876134
PRIMER_RIGHT_12_PENALTY=3.548339
PRIMER_LEFT_12_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_12_SEQUENCE=TAAAGGCCACCCTTTGGCTC
PRIMER_LEFT_12=839,20
PRIMER_RIGHT_12=1553,20
PRIMER_LEFT_12_TM=61.876
PRIMER_RIGHT_12_TM=63.548
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=715
PRIMER_PAIR_13_PENALTY=5.986079
PRIMER_LEFT_13_PENALTY=1.876134
PRIMER_RIGHT_13_PENALTY=4.109945
PRIMER_LEFT_13_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_13_SEQUENCE=CTTGATTACGCTAAAGGCCACC
PRIMER_LEFT_13=839,20
PRIMER_RIGHT_13=1564,22
PRIMER_LEFT_13_TM=61.876
PRIMER_RIGHT_13_TM=62.110
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=726
PRIMER_PAIR_14_PENALTY=6.045503
PRIMER_LEFT_14_PENALTY=1.876134
PRIMER_RIGHT_14_PENALTY=4.169369
PRIMER_LEFT_14_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_14_SEQUENCE=GAAACAAGCTATGAGGAGCTTACC
PRIMER_LEFT_14=839,20
PRIMER_RIGHT_14=1429,24
PRIMER_LEFT_14_TM=61.876
PRIMER_RIGHT_14_TM=59.831
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.833
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=591
PRIMER_PAIR_15_PENALTY=6.225865
PRIMER_LEFT_15_PENALTY=1.876134
PRIMER_RIGHT_15_PENALTY=4.349731
PRIMER_LEFT_15_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_15_SEQUENCE=TGATTACGCTAAAGGCCACCC
PRIMER_LEFT_15=839,20
PRIMER_RIGHT_15=1562,21
PRIMER_LEFT_15_TM=61.876
PRIMER_RIGHT_15_TM=63.350
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=724
PRIMER_PAIR_16_PENALTY=6.424641
PRIMER_LEFT_16_PENALTY=1.876134
PRIMER_RIGHT_16_PENALTY=4.548507
PRIMER_LEFT_16_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_16_SEQUENCE=TCTTCAGATCAGAGAATGTGGA
PRIMER_LEFT_16=839,20
PRIMER_RIGHT_16=1368,22
PRIMER_LEFT_16_TM=61.876
PRIMER_RIGHT_16_TM=57.451
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=530
PRIMER_PAIR_17_PENALTY=6.506116
PRIMER_LEFT_17_PENALTY=1.876134
PRIMER_RIGHT_17_PENALTY=4.629982
PRIMER_LEFT_17_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_17_SEQUENCE=GTGCTTGATGATGTGAATGA
PRIMER_LEFT_17=839,20
PRIMER_RIGHT_17=1804,20
PRIMER_LEFT_17_TM=61.876
PRIMER_RIGHT_17_TM=55.370
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=966
PRIMER_PAIR_18_PENALTY=6.613201
PRIMER_LEFT_18_PENALTY=1.876134
PRIMER_RIGHT_18_PENALTY=4.737068
PRIMER_LEFT_18_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_18_SEQUENCE=TTCTTGATTACGCTAAAGGCCAC
PRIMER_LEFT_18=839,20
PRIMER_RIGHT_18=1566,23
PRIMER_LEFT_18_TM=61.876
PRIMER_RIGHT_18_TM=61.737
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=728
PRIMER_PAIR_19_PENALTY=6.676280
PRIMER_LEFT_19_PENALTY=1.876134
PRIMER_RIGHT_19_PENALTY=4.800146
PRIMER_LEFT_19_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_19_SEQUENCE=AAACAAGCTATGAGGAGCTTACC
PRIMER_LEFT_19=839,20
PRIMER_RIGHT_19=1428,23
PRIMER_LEFT_19_TM=61.876
PRIMER_RIGHT_19_TM=58.200
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=7.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=590
PRIMER_PAIR_20_PENALTY=6.917797
PRIMER_LEFT_20_PENALTY=6.489316
PRIMER_RIGHT_20_PENALTY=0.428481
PRIMER_LEFT_20_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_20_SEQUENCE=TGTGGATTACGTGACAAGCC
PRIMER_LEFT_20=690,22
PRIMER_RIGHT_20=1352,20
PRIMER_LEFT_20_TM=55.511
PRIMER_RIGHT_20_TM=59.572
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.6000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=663
PRIMER_PAIR_21_PENALTY=7.002018
PRIMER_LEFT_21_PENALTY=1.876134
PRIMER_RIGHT_21_PENALTY=5.125884
PRIMER_LEFT_21_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_21_SEQUENCE=AACAAGCTATGAGGAGCTTACC
PRIMER_LEFT_21=839,20
PRIMER_RIGHT_21=1427,22
PRIMER_LEFT_21_TM=61.876
PRIMER_RIGHT_21_TM=56.874
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=7.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=589
PRIMER_PAIR_22_PENALTY=7.070183
PRIMER_LEFT_22_PENALTY=1.876134
PRIMER_RIGHT_22_PENALTY=5.194049
PRIMER_LEFT_22_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_22_SEQUENCE=GGTTCTTGATTACGCTAAAGG
PRIMER_LEFT_22=839,20
PRIMER_RIGHT_22=1568,21
PRIMER_LEFT_22_TM=61.876
PRIMER_RIGHT_22_TM=55.806
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.5000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=730
PRIMER_PAIR_23_PENALTY=7.139315
PRIMER_LEFT_23_PENALTY=1.876134
PRIMER_RIGHT_23_PENALTY=5.263181
PRIMER_LEFT_23_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_23_SEQUENCE=CTAAAGGCCACCCTTTGGCTC
PRIMER_LEFT_23=839,20
PRIMER_RIGHT_23=1554,21
PRIMER_LEFT_23_TM=61.876
PRIMER_RIGHT_23_TM=64.263
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=57.143
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=716
PRIMER_PAIR_24_PENALTY=7.251402
PRIMER_LEFT_24_PENALTY=1.876134
PRIMER_RIGHT_24_PENALTY=5.375268
PRIMER_LEFT_24_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_24_SEQUENCE=CACTCTTTCTGATAATCGCATC
PRIMER_LEFT_24=839,20
PRIMER_RIGHT_24=1268,22
PRIMER_LEFT_24_TM=61.876
PRIMER_RIGHT_24_TM=56.625
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=430
PRIMER_PAIR_25_PENALTY=7.310335
PRIMER_LEFT_25_PENALTY=6.489316
PRIMER_RIGHT_25_PENALTY=0.821019
PRIMER_LEFT_25_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_25_SEQUENCE=GATTACGCTAAAGGCCACCC
PRIMER_LEFT_25=690,22
PRIMER_RIGHT_25=1561,20
PRIMER_LEFT_25_TM=55.511
PRIMER_RIGHT_25_TM=60.821
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.6000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=872
PRIMER_PAIR_26_PENALTY=7.339978
PRIMER_LEFT_26_PENALTY=1.876134
PRIMER_RIGHT_26_PENALTY=5.463844
PRIMER_LEFT_26_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_26_SEQUENCE=GATCACTCTTTCTGATAATCGCATC
PRIMER_LEFT_26=839,20
PRIMER_RIGHT_26=1271,25
PRIMER_LEFT_26_TM=61.876
PRIMER_RIGHT_26_TM=60.464
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=433
PRIMER_PAIR_27_PENALTY=7.468963
PRIMER_LEFT_27_PENALTY=1.876134
PRIMER_RIGHT_27_PENALTY=5.592829
PRIMER_LEFT_27_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_27_SEQUENCE=ACAAGCTATGAGGAGCTTACC
PRIMER_LEFT_27=839,20
PRIMER_RIGHT_27=1426,21
PRIMER_LEFT_27_TM=61.876
PRIMER_RIGHT_27_TM=55.407
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=588
PRIMER_PAIR_28_PENALTY=7.525029
PRIMER_LEFT_28_PENALTY=1.876134
PRIMER_RIGHT_28_PENALTY=5.648895
PRIMER_LEFT_28_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_28_SEQUENCE=CTTCAGATCAGAGAATGTGGA
PRIMER_LEFT_28=839,20
PRIMER_RIGHT_28=1367,21
PRIMER_LEFT_28_TM=61.876
PRIMER_RIGHT_28_TM=55.351
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=529
PRIMER_PAIR_29_PENALTY=7.525029
PRIMER_LEFT_29_PENALTY=1.876134
PRIMER_RIGHT_29_PENALTY=5.648895
PRIMER_LEFT_29_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_29_SEQUENCE=TCTTCAGATCAGAGAATGTGG
PRIMER_LEFT_29=839,20
PRIMER_RIGHT_29=1368,21
PRIMER_LEFT_29_TM=61.876
PRIMER_RIGHT_29_TM=55.351
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=530
PRIMER_PAIR_30_PENALTY=7.615509
PRIMER_LEFT_30_PENALTY=6.489316
PRIMER_RIGHT_30_PENALTY=1.126193
PRIMER_LEFT_30_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_30_SEQUENCE=ATGTGGATTACGTGACAAGCC
PRIMER_LEFT_30=690,22
PRIMER_RIGHT_30=1353,21
PRIMER_LEFT_30_TM=55.511
PRIMER_RIGHT_30_TM=59.874
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=664
PRIMER_PAIR_31_PENALTY=7.710530
PRIMER_LEFT_31_PENALTY=1.876134
PRIMER_RIGHT_31_PENALTY=5.834396
PRIMER_LEFT_31_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_31_SEQUENCE=GAATGTGGATTACGTGACAAGCC
PRIMER_LEFT_31=839,20
PRIMER_RIGHT_31=1355,23
PRIMER_LEFT_31_TM=61.876
PRIMER_RIGHT_31_TM=62.834
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.826
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=517
PRIMER_PAIR_32_PENALTY=8.229672
PRIMER_LEFT_32_PENALTY=1.876134
PRIMER_RIGHT_32_PENALTY=6.353538
PRIMER_LEFT_32_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_32_SEQUENCE=TTGATTACGCTAAAGGCCACCC
PRIMER_LEFT_32=839,20
PRIMER_RIGHT_32=1563,22
PRIMER_LEFT_32_TM=61.876
PRIMER_RIGHT_32_TM=64.354
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=9.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=725
PRIMER_PAIR_33_PENALTY=8.245756
PRIMER_LEFT_33_PENALTY=1.876134
PRIMER_RIGHT_33_PENALTY=6.369622
PRIMER_LEFT_33_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_33_SEQUENCE=GTTCTTGATTACGCTAAAGGCCAC
PRIMER_LEFT_33=839,20
PRIMER_RIGHT_33=1567,24
PRIMER_LEFT_33_TM=61.876
PRIMER_RIGHT_33_TM=62.370
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.833
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=729
PRIMER_PAIR_34_PENALTY=8.275253
PRIMER_LEFT_34_PENALTY=1.876134
PRIMER_RIGHT_34_PENALTY=6.399119
PRIMER_LEFT_34_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_34_SEQUENCE=GAAACAAGCTATGAGGAGCTTAC
PRIMER_LEFT_34=839,20
PRIMER_RIGHT_34=1429,23
PRIMER_LEFT_34_TM=61.876
PRIMER_RIGHT_34_TM=56.601
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=43.478
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=5.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=591
PRIMER_PAIR_35_PENALTY=8.515880
PRIMER_LEFT_35_PENALTY=1.876134
PRIMER_RIGHT_35_PENALTY=6.639746
PRIMER_LEFT_35_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_35_SEQUENCE=TCTTGATTACGCTAAAGGCCACC
PRIMER_LEFT_35=839,20
PRIMER_RIGHT_35=1565,23
PRIMER_LEFT_35_TM=61.876
PRIMER_RIGHT_35_TM=63.640
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.826
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=727
PRIMER_PAIR_36_PENALTY=8.518297
PRIMER_LEFT_36_PENALTY=1.876134
PRIMER_RIGHT_36_PENALTY=6.642163
PRIMER_LEFT_36_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_36_SEQUENCE=CTTCAGATCAGAGAATGTGGATTAC
PRIMER_LEFT_36=839,20
PRIMER_RIGHT_36=1367,25
PRIMER_LEFT_36_TM=61.876
PRIMER_RIGHT_36_TM=58.358
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=5.6000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=529
PRIMER_PAIR_37_PENALTY=9.158279
PRIMER_LEFT_37_PENALTY=6.489316
PRIMER_RIGHT_37_PENALTY=2.668963
PRIMER_LEFT_37_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_37_SEQUENCE=CTAAAGGCCACCCTTTGGCT
PRIMER_LEFT_37=690,22
PRIMER_RIGHT_37=1554,20
PRIMER_LEFT_37_TM=55.511
PRIMER_RIGHT_37_TM=62.669
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=865
PRIMER_PAIR_38_PENALTY=9.443557
PRIMER_LEFT_38_PENALTY=1.876134
PRIMER_RIGHT_38_PENALTY=7.567423
PRIMER_LEFT_38_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_38_SEQUENCE=AGAATGTGGATTACGTGACAAGCC
PRIMER_LEFT_38=839,20
PRIMER_RIGHT_38=1356,24
PRIMER_LEFT_38_TM=61.876
PRIMER_RIGHT_38_TM=63.567
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=45.833
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=518
PRIMER_PAIR_39_PENALTY=9.643887
PRIMER_LEFT_39_PENALTY=6.489316
PRIMER_RIGHT_39_PENALTY=3.154571
PRIMER_LEFT_39_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_39_SEQUENCE=AATGTGGATTACGTGACAAGCC
PRIMER_LEFT_39=690,22
PRIMER_RIGHT_39=1354,22
PRIMER_LEFT_39_TM=55.511
PRIMER_RIGHT_39_TM=61.155
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=665
PRIMER_PAIR_40_PENALTY=9.860120
PRIMER_LEFT_40_PENALTY=1.876134
PRIMER_RIGHT_40_PENALTY=7.983986
PRIMER_LEFT_40_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_40_SEQUENCE=CTTGATTACGCTAAAGGCCACCC
PRIMER_LEFT_40=839,20
PRIMER_RIGHT_40=1564,23
PRIMER_LEFT_40_TM=61.876
PRIMER_RIGHT_40_TM=64.984
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=52.174
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=9.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=726
PRIMER_PAIR_41_PENALTY=10.033063
PRIMER_LEFT_41_PENALTY=6.489316
PRIMER_RIGHT_41_PENALTY=3.543747
PRIMER_LEFT_41_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_41_SEQUENCE=TGGTTCTTGATTACGCTAAAGG
PRIMER_LEFT_41=690,22
PRIMER_RIGHT_41=1569,22
PRIMER_LEFT_41_TM=55.511
PRIMER_RIGHT_41_TM=58.456
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=8.5000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=880
PRIMER_PAIR_42_PENALTY=10.037655
PRIMER_LEFT_42_PENALTY=6.489316
PRIMER_RIGHT_42_PENALTY=3.548339
PRIMER_LEFT_42_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_42_SEQUENCE=TAAAGGCCACCCTTTGGCTC
PRIMER_LEFT_42=690,22
PRIMER_RIGHT_42=1553,20
PRIMER_LEFT_42_TM=55.511
PRIMER_RIGHT_42_TM=63.548
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=864
PRIMER_PAIR_43_PENALTY=10.447261
PRIMER_LEFT_43_PENALTY=1.876134
PRIMER_RIGHT_43_PENALTY=8.571127
PRIMER_LEFT_43_SEQUENCE=CATCCACGGGAACAATGAGA
PRIMER_RIGHT_43_SEQUENCE=TTCTTGATTACGCTAAAGGCCACC
PRIMER_LEFT_43=839,20
PRIMER_RIGHT_43=1566,24
PRIMER_LEFT_43_TM=61.876
PRIMER_RIGHT_43_TM=64.571
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.833
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=728
PRIMER_PAIR_44_PENALTY=10.658685
PRIMER_LEFT_44_PENALTY=6.489316
PRIMER_RIGHT_44_PENALTY=4.169369
PRIMER_LEFT_44_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_44_SEQUENCE=GAAACAAGCTATGAGGAGCTTACC
PRIMER_LEFT_44=690,22
PRIMER_RIGHT_44=1429,24
PRIMER_LEFT_44_TM=55.511
PRIMER_RIGHT_44_TM=59.831
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=45.833
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=7.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=740
PRIMER_PAIR_45_PENALTY=10.839047
PRIMER_LEFT_45_PENALTY=6.489316
PRIMER_RIGHT_45_PENALTY=4.349731
PRIMER_LEFT_45_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_45_SEQUENCE=TGATTACGCTAAAGGCCACCC
PRIMER_LEFT_45=690,22
PRIMER_RIGHT_45=1562,21
PRIMER_LEFT_45_TM=55.511
PRIMER_RIGHT_45_TM=63.350
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.6000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=873
PRIMER_PAIR_46_PENALTY=11.037823
PRIMER_LEFT_46_PENALTY=6.489316
PRIMER_RIGHT_46_PENALTY=4.548507
PRIMER_LEFT_46_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_46_SEQUENCE=TCTTCAGATCAGAGAATGTGGA
PRIMER_LEFT_46=690,22
PRIMER_RIGHT_46=1368,22
PRIMER_LEFT_46_TM=55.511
PRIMER_RIGHT_46_TM=57.451
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.6000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=679
PRIMER_PAIR_47_PENALTY=11.289462
PRIMER_LEFT_47_PENALTY=6.489316
PRIMER_RIGHT_47_PENALTY=4.800146
PRIMER_LEFT_47_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_47_SEQUENCE=AAACAAGCTATGAGGAGCTTACC
PRIMER_LEFT_47=690,22
PRIMER_RIGHT_47=1428,23
PRIMER_LEFT_47_TM=55.511
PRIMER_RIGHT_47_TM=58.200
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=7.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=739
PRIMER_PAIR_48_PENALTY=11.615201
PRIMER_LEFT_48_PENALTY=6.489316
PRIMER_RIGHT_48_PENALTY=5.125884
PRIMER_LEFT_48_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_48_SEQUENCE=AACAAGCTATGAGGAGCTTACC
PRIMER_LEFT_48=690,22
PRIMER_RIGHT_48=1427,22
PRIMER_LEFT_48_TM=55.511
PRIMER_RIGHT_48_TM=56.874
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=7.2000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=738
PRIMER_PAIR_49_PENALTY=11.683365
PRIMER_LEFT_49_PENALTY=6.489316
PRIMER_RIGHT_49_PENALTY=5.194049
PRIMER_LEFT_49_SEQUENCE=GACTATGTGGCAATTTAAGGTC
PRIMER_RIGHT_49_SEQUENCE=GGTTCTTGATTACGCTAAAGG
PRIMER_LEFT_49=690,22
PRIMER_RIGHT_49=1568,21
PRIMER_LEFT_49_TM=55.511
PRIMER_RIGHT_49_TM=55.806
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=879
=
SEQUENCE_ID=MAGIC.446_4_1626406_1626434_po-0_edi-0_62617_798_TRUE
SEQUENCE_TARGET=950,128
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_5185501_5185682_can-0_po-0_2302_12947_TRUE
SEQUENCE_TARGET=950,281
SEQUENCE_TEMPLATE=GANGCTAAACTNNAACANACTTTNAGAATATTTGAGACGATNAACTGGTCNGCATCGAGTCCCAACTTGNTTAATTCNCTGAANAGATAAAATGCTAAAGAATTGAACCCTGATTTCACACANCCTCGTATCAAGCCAGAGAAAGCGATGATATCTTTATTTGGAAGCNNATGAAACAGNTTATGTGCNTCTTGTATATTCCCAACNTTTGCNTGTANATCAACAAGAATNCTTCCTACNATGTAATCCAACTCATAACCACTTACTACAACTAAACTATGTACTTGAAGCCCGAGTCTTAAGTTGACNNAGTTGATACATATCTTTAAGGCACCACTTAGAGTGTAGGAATCAAAANANAAATCTGACTGANANATCTGCAAAAGNAGCCNTAATGCTGCTTCATTCTCNTCNTTNATCAAAAATCCAGAGAGCATAGAATTCCAAACNGNGANACTANTANNNNNNNNNNGNTTTTCCTGATGAAAAACNTCAGCTGCATNNNTTAGAGANCCACAGTTNGAGTACATATCAATAAGAGCAGANATTGCGAACGGGCTAGACTCTAGACCCGACTTTNCGACACAACAATGCAGCTGTTTACCCATTGTTAATAANCCACCAAAACTACANGCTTTAAGACCACANGGTAAAGCAAAACCGTCTAACACAAGNCCTTCTCTCTGCATCNNAACTAAAAACTCCAACGCACNAGGACTACCCTTATCGACAAATCCCGATANCAAACAGTTCCAAGACACGACATTTGGCNGCGGCATTNGATGNAACAAANTAACNGCTTCATCNATCAAACCAGCTTTACAATAACCAGAAATAAGAGTGTTCCACGAAGTCGAACTAGGCCGTAAAATCTCCTTAAAANTGCTANTAGCTTCNNTAAGTCTCCCATTCTTAACATACATATCNACAACACTATTCATCAAAACAACATCACCNCTCAAATTCTCTTNACCAATTCTTNCATAAACCAAANTCCCNANCTGAATATCACCCACTAACCCACAAGCTTTCAAAACCGCAGAATACATAAACTCGTTCGCNGCTTCTTCTTCGGAGTCTAACATTCTTCTGTATAACTCAATAGCTTTATTGGGTTTTCCATCACTCGTATACCCAGAAACCATCGTTGTCCAAGTTACAATATTTCTCTCANACATTTCNTCGAACACTTTATGTGCATCACTCAACAANCTNAAATCCACATACATTGAGATCACGTTATTCGCTATAAACACATTTTGAGAGATCCCTTGCTTGATTACATGAGCTTGANTTGATTCTCCACGCTTAAAGGCTTGTACTTTCCCACAATGACGTANTCCTGCTGCTATANGCTTCAAGTCCANAACCATTCTCCAGTANGAAAATTTTNGACAATNNCGTTACATTTACAAATTGNNTANTCTCCACGTAAAACGGAAGGTAGTTTTATNGAAAANNTNAAAACGGCACGCTGTTTAGTACATGAATTNANGACNGTANAAACGGTNGGTAGTTTGNTTGGTAANNNGNAAACNGCANGCTGTTCAATACGNTAGTGGCTGNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNAANNNNTTNNCNNNNNNNGTAAAAACGGAAGGTNGTTTNTTTNAGAAAATAAAAACGTTACNNTGNTNANTACNNNANGGAAGATNGTAAAACGNCACNATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTGTGTTGTGGAAATTCAAAACGGCACNNTGTTNAACATNGGNGTGAAGAANAATCTCATAATTTAGGATCAATGGCTGATNAAAAAAAAANCAGATCTNAAANNNNNTCCGTCGTCGCCGTCTTCCTTCACGANNNNCTCCTCTCTTCCNAAACTTCAATCTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.904112
PRIMER_LEFT_0_PENALTY=0.801088
PRIMER_RIGHT_0_PENALTY=0.103024
PRIMER_LEFT_0_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_0_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_0=591,20
PRIMER_RIGHT_0=1443,20
PRIMER_LEFT_0_TM=59.199
PRIMER_RIGHT_0_TM=60.103
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=853
PRIMER_PAIR_1_PENALTY=1.113553
PRIMER_LEFT_1_PENALTY=1.010529
PRIMER_RIGHT_1_PENALTY=0.103024
PRIMER_LEFT_1_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_1_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_1=588,21
PRIMER_RIGHT_1=1443,20
PRIMER_LEFT_1_TM=60.011
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=1.314806
PRIMER_LEFT_2_PENALTY=0.801088
PRIMER_RIGHT_2_PENALTY=0.513718
PRIMER_LEFT_2_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_2_SEQUENCE=CAAGCCTTTAAGCGTGGAGA
PRIMER_LEFT_2=591,20
PRIMER_RIGHT_2=1318,20
PRIMER_LEFT_2_TM=59.199
PRIMER_RIGHT_2_TM=60.514
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=728
PRIMER_PAIR_3_PENALTY=1.524246
PRIMER_LEFT_3_PENALTY=1.010529
PRIMER_RIGHT_3_PENALTY=0.513718
PRIMER_LEFT_3_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_3_SEQUENCE=CAAGCCTTTAAGCGTGGAGA
PRIMER_LEFT_3=588,21
PRIMER_RIGHT_3=1318,20
PRIMER_LEFT_3_TM=60.011
PRIMER_RIGHT_3_TM=60.514
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=731
PRIMER_PAIR_4_PENALTY=1.590253
PRIMER_LEFT_4_PENALTY=1.487228
PRIMER_RIGHT_4_PENALTY=0.103024
PRIMER_LEFT_4_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_4_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_4=590,21
PRIMER_RIGHT_4=1443,20
PRIMER_LEFT_4_TM=59.513
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=854
PRIMER_PAIR_5_PENALTY=1.651962
PRIMER_LEFT_5_PENALTY=1.548938
PRIMER_RIGHT_5_PENALTY=0.103024
PRIMER_LEFT_5_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_5_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_5=585,21
PRIMER_RIGHT_5=1443,20
PRIMER_LEFT_5_TM=60.549
PRIMER_RIGHT_5_TM=60.103
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=859
PRIMER_PAIR_6_PENALTY=1.690146
PRIMER_LEFT_6_PENALTY=0.801088
PRIMER_RIGHT_6_PENALTY=0.889058
PRIMER_LEFT_6_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_6_SEQUENCE=AAGCCTTTAAGCGTGGAGAA
PRIMER_LEFT_6=591,20
PRIMER_RIGHT_6=1317,20
PRIMER_LEFT_6_TM=59.199
PRIMER_RIGHT_6_TM=59.111
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=727
PRIMER_PAIR_7_PENALTY=1.860618
PRIMER_LEFT_7_PENALTY=0.801088
PRIMER_RIGHT_7_PENALTY=1.059530
PRIMER_LEFT_7_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_7_SEQUENCE=TCAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_7=591,20
PRIMER_RIGHT_7=1278,21
PRIMER_LEFT_7_TM=59.199
PRIMER_RIGHT_7_TM=59.940
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=688
PRIMER_PAIR_8_PENALTY=1.899587
PRIMER_LEFT_8_PENALTY=1.010529
PRIMER_RIGHT_8_PENALTY=0.889058
PRIMER_LEFT_8_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_8_SEQUENCE=AAGCCTTTAAGCGTGGAGAA
PRIMER_LEFT_8=588,21
PRIMER_RIGHT_8=1317,20
PRIMER_LEFT_8_TM=60.011
PRIMER_RIGHT_8_TM=59.111
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=730
PRIMER_PAIR_9_PENALTY=2.000946
PRIMER_LEFT_9_PENALTY=1.487228
PRIMER_RIGHT_9_PENALTY=0.513718
PRIMER_LEFT_9_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_9_SEQUENCE=CAAGCCTTTAAGCGTGGAGA
PRIMER_LEFT_9=590,21
PRIMER_RIGHT_9=1318,20
PRIMER_LEFT_9_TM=59.513
PRIMER_RIGHT_9_TM=60.514
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=729
PRIMER_PAIR_10_PENALTY=2.062656
PRIMER_LEFT_10_PENALTY=1.548938
PRIMER_RIGHT_10_PENALTY=0.513718
PRIMER_LEFT_10_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_10_SEQUENCE=CAAGCCTTTAAGCGTGGAGA
PRIMER_LEFT_10=585,21
PRIMER_RIGHT_10=1318,20
PRIMER_LEFT_10_TM=60.549
PRIMER_RIGHT_10_TM=60.514
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=734
PRIMER_PAIR_11_PENALTY=2.070059
PRIMER_LEFT_11_PENALTY=1.010529
PRIMER_RIGHT_11_PENALTY=1.059530
PRIMER_LEFT_11_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_11_SEQUENCE=TCAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_11=588,21
PRIMER_RIGHT_11=1278,21
PRIMER_LEFT_11_TM=60.011
PRIMER_RIGHT_11_TM=59.940
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=691
PRIMER_PAIR_12_PENALTY=2.079376
PRIMER_LEFT_12_PENALTY=0.801088
PRIMER_RIGHT_12_PENALTY=1.278288
PRIMER_LEFT_12_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_12_SEQUENCE=GCTCATGTAATCAAGCAAGGG
PRIMER_LEFT_12=591,20
PRIMER_RIGHT_12=1288,21
PRIMER_LEFT_12_TM=59.199
PRIMER_RIGHT_12_TM=59.722
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=698
PRIMER_PAIR_13_PENALTY=2.286036
PRIMER_LEFT_13_PENALTY=0.801088
PRIMER_RIGHT_13_PENALTY=1.484948
PRIMER_LEFT_13_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_13_SEQUENCE=TCAAGCAAGGGATCTCTCAA
PRIMER_LEFT_13=591,20
PRIMER_RIGHT_13=1278,20
PRIMER_LEFT_13_TM=59.199
PRIMER_RIGHT_13_TM=58.515
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=688
PRIMER_PAIR_14_PENALTY=2.288817
PRIMER_LEFT_14_PENALTY=1.010529
PRIMER_RIGHT_14_PENALTY=1.278288
PRIMER_LEFT_14_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_14_SEQUENCE=GCTCATGTAATCAAGCAAGGG
PRIMER_LEFT_14=588,21
PRIMER_RIGHT_14=1288,21
PRIMER_LEFT_14_TM=60.011
PRIMER_RIGHT_14_TM=59.722
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=701
PRIMER_PAIR_15_PENALTY=2.348109
PRIMER_LEFT_15_PENALTY=2.245085
PRIMER_RIGHT_15_PENALTY=0.103024
PRIMER_LEFT_15_SEQUENCE=AACAATGCAGCTGTTTACCC
PRIMER_RIGHT_15_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_15=586,20
PRIMER_RIGHT_15=1443,20
PRIMER_LEFT_15_TM=57.755
PRIMER_RIGHT_15_TM=60.103
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=858
PRIMER_PAIR_16_PENALTY=2.362611
PRIMER_LEFT_16_PENALTY=0.801088
PRIMER_RIGHT_16_PENALTY=1.561523
PRIMER_LEFT_16_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_16_SEQUENCE=CAAGCAAGGGATCTCTCAAAA
PRIMER_LEFT_16=591,20
PRIMER_RIGHT_16=1277,21
PRIMER_LEFT_16_TM=59.199
PRIMER_RIGHT_16_TM=59.438
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=687
PRIMER_PAIR_17_PENALTY=2.376287
PRIMER_LEFT_17_PENALTY=1.487228
PRIMER_RIGHT_17_PENALTY=0.889058
PRIMER_LEFT_17_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_17_SEQUENCE=AAGCCTTTAAGCGTGGAGAA
PRIMER_LEFT_17=590,21
PRIMER_RIGHT_17=1317,20
PRIMER_LEFT_17_TM=59.513
PRIMER_RIGHT_17_TM=59.111
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=728
PRIMER_PAIR_18_PENALTY=2.437996
PRIMER_LEFT_18_PENALTY=1.548938
PRIMER_RIGHT_18_PENALTY=0.889058
PRIMER_LEFT_18_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_18_SEQUENCE=AAGCCTTTAAGCGTGGAGAA
PRIMER_LEFT_18=585,21
PRIMER_RIGHT_18=1317,20
PRIMER_LEFT_18_TM=60.549
PRIMER_RIGHT_18_TM=59.111
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=733
PRIMER_PAIR_19_PENALTY=2.453511
PRIMER_LEFT_19_PENALTY=0.801088
PRIMER_RIGHT_19_PENALTY=1.652423
PRIMER_LEFT_19_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_19_SEQUENCE=CCTTTAAGCGTGGAGAATCAA
PRIMER_LEFT_19=591,20
PRIMER_RIGHT_19=1314,21
PRIMER_LEFT_19_TM=59.199
PRIMER_RIGHT_19_TM=59.348
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=724
PRIMER_PAIR_20_PENALTY=2.495477
PRIMER_LEFT_20_PENALTY=1.010529
PRIMER_RIGHT_20_PENALTY=1.484948
PRIMER_LEFT_20_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_20_SEQUENCE=TCAAGCAAGGGATCTCTCAA
PRIMER_LEFT_20=588,21
PRIMER_RIGHT_20=1278,20
PRIMER_LEFT_20_TM=60.011
PRIMER_RIGHT_20_TM=58.515
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.4000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=691
PRIMER_PAIR_21_PENALTY=2.546758
PRIMER_LEFT_21_PENALTY=1.487228
PRIMER_RIGHT_21_PENALTY=1.059530
PRIMER_LEFT_21_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_21_SEQUENCE=TCAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_21=590,21
PRIMER_RIGHT_21=1278,21
PRIMER_LEFT_21_TM=59.513
PRIMER_RIGHT_21_TM=59.940
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=7.3000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=689
PRIMER_PAIR_22_PENALTY=2.556685
PRIMER_LEFT_22_PENALTY=1.497155
PRIMER_RIGHT_22_PENALTY=1.059530
PRIMER_LEFT_22_SEQUENCE=TGAAGCCCGAGTCTTAAGTTG
PRIMER_RIGHT_22_SEQUENCE=TCAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_22=285,21
PRIMER_RIGHT_22=1278,21
PRIMER_LEFT_22_TM=59.503
PRIMER_RIGHT_22_TM=59.940
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=994
PRIMER_PAIR_23_PENALTY=2.572052
PRIMER_LEFT_23_PENALTY=1.010529
PRIMER_RIGHT_23_PENALTY=1.561523
PRIMER_LEFT_23_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_23_SEQUENCE=CAAGCAAGGGATCTCTCAAAA
PRIMER_LEFT_23=588,21
PRIMER_RIGHT_23=1277,21
PRIMER_LEFT_23_TM=60.011
PRIMER_RIGHT_23_TM=59.438
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=690
PRIMER_PAIR_24_PENALTY=2.575323
PRIMER_LEFT_24_PENALTY=2.472299
PRIMER_RIGHT_24_PENALTY=0.103024
PRIMER_LEFT_24_SEQUENCE=GAAGTCGAACTAGGCCGTAAA
PRIMER_RIGHT_24_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_24=849,21
PRIMER_RIGHT_24=1443,20
PRIMER_LEFT_24_TM=58.528
PRIMER_RIGHT_24_TM=60.103
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.0000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=595
PRIMER_PAIR_25_PENALTY=2.608468
PRIMER_LEFT_25_PENALTY=1.548938
PRIMER_RIGHT_25_PENALTY=1.059530
PRIMER_LEFT_25_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_25_SEQUENCE=TCAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_25=585,21
PRIMER_RIGHT_25=1278,21
PRIMER_LEFT_25_TM=60.549
PRIMER_RIGHT_25_TM=59.940
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.4000
PRIMER_RIGHT_25_END_STABILITY=7.3000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=694
PRIMER_PAIR_26_PENALTY=2.662951
PRIMER_LEFT_26_PENALTY=1.010529
PRIMER_RIGHT_26_PENALTY=1.652423
PRIMER_LEFT_26_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_26_SEQUENCE=CCTTTAAGCGTGGAGAATCAA
PRIMER_LEFT_26=588,21
PRIMER_RIGHT_26=1314,21
PRIMER_LEFT_26_TM=60.011
PRIMER_RIGHT_26_TM=59.348
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=727
PRIMER_PAIR_27_PENALTY=2.690022
PRIMER_LEFT_27_PENALTY=0.801088
PRIMER_RIGHT_27_PENALTY=1.888934
PRIMER_LEFT_27_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_27_SEQUENCE=ACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_27=591,20
PRIMER_RIGHT_27=1444,21
PRIMER_LEFT_27_TM=59.199
PRIMER_RIGHT_27_TM=60.889
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.6000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=854
PRIMER_PAIR_28_PENALTY=2.698281
PRIMER_LEFT_28_PENALTY=2.595257
PRIMER_RIGHT_28_PENALTY=0.103024
PRIMER_LEFT_28_SEQUENCE=CACAACAATGCAGCTGTTTACC
PRIMER_RIGHT_28_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_28=583,22
PRIMER_RIGHT_28=1443,20
PRIMER_LEFT_28_TM=60.595
PRIMER_RIGHT_28_TM=60.103
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=861
PRIMER_PAIR_29_PENALTY=2.720577
PRIMER_LEFT_29_PENALTY=2.206859
PRIMER_RIGHT_29_PENALTY=0.513718
PRIMER_LEFT_29_SEQUENCE=GAAGTCGAACTAGGCCGTAAAA
PRIMER_RIGHT_29_SEQUENCE=CAAGCCTTTAAGCGTGGAGA
PRIMER_LEFT_29=849,22
PRIMER_RIGHT_29=1318,20
PRIMER_LEFT_29_TM=59.793
PRIMER_RIGHT_29_TM=60.514
PRIMER_LEFT_29_GC_PERCENT=45.455
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=470
PRIMER_PAIR_30_PENALTY=2.758803
PRIMER_LEFT_30_PENALTY=2.245085
PRIMER_RIGHT_30_PENALTY=0.513718
PRIMER_LEFT_30_SEQUENCE=AACAATGCAGCTGTTTACCC
PRIMER_RIGHT_30_SEQUENCE=CAAGCCTTTAAGCGTGGAGA
PRIMER_LEFT_30=586,20
PRIMER_RIGHT_30=1318,20
PRIMER_LEFT_30_TM=57.755
PRIMER_RIGHT_30_TM=60.514
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.4000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=733
PRIMER_PAIR_31_PENALTY=2.765516
PRIMER_LEFT_31_PENALTY=1.487228
PRIMER_RIGHT_31_PENALTY=1.278288
PRIMER_LEFT_31_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_31_SEQUENCE=GCTCATGTAATCAAGCAAGGG
PRIMER_LEFT_31=590,21
PRIMER_RIGHT_31=1288,21
PRIMER_LEFT_31_TM=59.513
PRIMER_RIGHT_31_TM=59.722
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.6000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=699
PRIMER_PAIR_32_PENALTY=2.781123
PRIMER_LEFT_32_PENALTY=0.801088
PRIMER_RIGHT_32_PENALTY=1.980035
PRIMER_LEFT_32_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_32_SEQUENCE=CAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_32=591,20
PRIMER_RIGHT_32=1277,20
PRIMER_LEFT_32_TM=59.199
PRIMER_RIGHT_32_TM=58.020
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=687
PRIMER_PAIR_33_PENALTY=2.827226
PRIMER_LEFT_33_PENALTY=1.548938
PRIMER_RIGHT_33_PENALTY=1.278288
PRIMER_LEFT_33_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_33_SEQUENCE=GCTCATGTAATCAAGCAAGGG
PRIMER_LEFT_33=585,21
PRIMER_RIGHT_33=1288,21
PRIMER_LEFT_33_TM=60.549
PRIMER_RIGHT_33_TM=59.722
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=704
PRIMER_PAIR_34_PENALTY=2.827622
PRIMER_LEFT_34_PENALTY=2.724598
PRIMER_RIGHT_34_PENALTY=0.103024
PRIMER_LEFT_34_SEQUENCE=CAAACAGTTCCAAGACACGA
PRIMER_RIGHT_34_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_34=743,20
PRIMER_RIGHT_34=1443,20
PRIMER_LEFT_34_TM=57.275
PRIMER_RIGHT_34_TM=60.103
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.4000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=701
PRIMER_PAIR_35_PENALTY=2.829019
PRIMER_LEFT_35_PENALTY=0.801088
PRIMER_RIGHT_35_PENALTY=2.027931
PRIMER_LEFT_35_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_35_SEQUENCE=CCTTTAAGCGTGGAGAATCA
PRIMER_LEFT_35=591,20
PRIMER_RIGHT_35=1314,20
PRIMER_LEFT_35_TM=59.199
PRIMER_RIGHT_35_TM=57.972
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=724
PRIMER_PAIR_36_PENALTY=2.845698
PRIMER_LEFT_36_PENALTY=2.742674
PRIMER_RIGHT_36_PENALTY=0.103024
PRIMER_LEFT_36_SEQUENCE=AAACAGTTCCAAGACACGACA
PRIMER_RIGHT_36_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_36=744,21
PRIMER_RIGHT_36=1443,20
PRIMER_LEFT_36_TM=58.257
PRIMER_RIGHT_36_TM=60.103
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.4000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=700
PRIMER_PAIR_37_PENALTY=2.845698
PRIMER_LEFT_37_PENALTY=2.742674
PRIMER_RIGHT_37_PENALTY=0.103024
PRIMER_LEFT_37_SEQUENCE=CAAACAGTTCCAAGACACGAC
PRIMER_RIGHT_37_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_37=743,21
PRIMER_RIGHT_37=1443,20
PRIMER_LEFT_37_TM=58.257
PRIMER_RIGHT_37_TM=60.103
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.8000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=701
PRIMER_PAIR_38_PENALTY=2.860515
PRIMER_LEFT_38_PENALTY=2.757491
PRIMER_RIGHT_38_PENALTY=0.103024
PRIMER_LEFT_38_SEQUENCE=AATGCAGCTGTTTACCCATT
PRIMER_RIGHT_38_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_38=589,20
PRIMER_RIGHT_38=1443,20
PRIMER_LEFT_38_TM=57.243
PRIMER_RIGHT_38_TM=60.103
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.4000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=855
PRIMER_PAIR_39_PENALTY=2.881550
PRIMER_LEFT_39_PENALTY=2.778526
PRIMER_RIGHT_39_PENALTY=0.103024
PRIMER_LEFT_39_SEQUENCE=AATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_39_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_39=589,22
PRIMER_RIGHT_39=1443,20
PRIMER_LEFT_39_TM=60.779
PRIMER_RIGHT_39_TM=60.103
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=855
PRIMER_PAIR_40_PENALTY=2.881550
PRIMER_LEFT_40_PENALTY=2.778526
PRIMER_RIGHT_40_PENALTY=0.103024
PRIMER_LEFT_40_SEQUENCE=ACAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_40_SEQUENCE=CCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_40=587,22
PRIMER_RIGHT_40=1443,20
PRIMER_LEFT_40_TM=60.779
PRIMER_RIGHT_40_TM=60.103
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.4000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=857
PRIMER_PAIR_41_PENALTY=2.899463
PRIMER_LEFT_41_PENALTY=1.010529
PRIMER_RIGHT_41_PENALTY=1.888934
PRIMER_LEFT_41_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_41_SEQUENCE=ACCTTCCGTTTTACGTGGAGA
PRIMER_LEFT_41=588,21
PRIMER_RIGHT_41=1444,21
PRIMER_LEFT_41_TM=60.011
PRIMER_RIGHT_41_TM=60.889
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.4000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=857
PRIMER_PAIR_42_PENALTY=2.925914
PRIMER_LEFT_42_PENALTY=0.801088
PRIMER_RIGHT_42_PENALTY=2.124825
PRIMER_LEFT_42_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_42_SEQUENCE=ATCAAGCAAGGGATCTCTCAA
PRIMER_LEFT_42=591,20
PRIMER_RIGHT_42=1279,21
PRIMER_LEFT_42_TM=59.199
PRIMER_RIGHT_42_TM=58.875
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.6000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=689
PRIMER_PAIR_43_PENALTY=2.972177
PRIMER_LEFT_43_PENALTY=1.487228
PRIMER_RIGHT_43_PENALTY=1.484948
PRIMER_LEFT_43_SEQUENCE=ATGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_43_SEQUENCE=TCAAGCAAGGGATCTCTCAA
PRIMER_LEFT_43=590,21
PRIMER_RIGHT_43=1278,20
PRIMER_LEFT_43_TM=59.513
PRIMER_RIGHT_43_TM=58.515
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=689
PRIMER_PAIR_44_PENALTY=2.982103
PRIMER_LEFT_44_PENALTY=1.497155
PRIMER_RIGHT_44_PENALTY=1.484948
PRIMER_LEFT_44_SEQUENCE=TGAAGCCCGAGTCTTAAGTTG
PRIMER_RIGHT_44_SEQUENCE=TCAAGCAAGGGATCTCTCAA
PRIMER_LEFT_44=285,21
PRIMER_RIGHT_44=1278,20
PRIMER_LEFT_44_TM=59.503
PRIMER_RIGHT_44_TM=58.515
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=994
PRIMER_PAIR_45_PENALTY=2.986017
PRIMER_LEFT_45_PENALTY=2.472299
PRIMER_RIGHT_45_PENALTY=0.513718
PRIMER_LEFT_45_SEQUENCE=GAAGTCGAACTAGGCCGTAAA
PRIMER_RIGHT_45_SEQUENCE=CAAGCCTTTAAGCGTGGAGA
PRIMER_LEFT_45=849,21
PRIMER_RIGHT_45=1318,20
PRIMER_LEFT_45_TM=58.528
PRIMER_RIGHT_45_TM=60.514
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.0000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=470
PRIMER_PAIR_46_PENALTY=2.990564
PRIMER_LEFT_46_PENALTY=1.010529
PRIMER_RIGHT_46_PENALTY=1.980035
PRIMER_LEFT_46_SEQUENCE=CAATGCAGCTGTTTACCCATT
PRIMER_RIGHT_46_SEQUENCE=CAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_46=588,21
PRIMER_RIGHT_46=1277,20
PRIMER_LEFT_46_TM=60.011
PRIMER_RIGHT_46_TM=58.020
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.4000
PRIMER_RIGHT_46_END_STABILITY=7.3000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=690
PRIMER_PAIR_47_PENALTY=3.008844
PRIMER_LEFT_47_PENALTY=0.801088
PRIMER_RIGHT_47_PENALTY=2.207756
PRIMER_LEFT_47_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_47_SEQUENCE=ATCAAGCAAGGGATCTCTCAAA
PRIMER_LEFT_47=591,20
PRIMER_RIGHT_47=1279,22
PRIMER_LEFT_47_TM=59.199
PRIMER_RIGHT_47_TM=60.208
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=689
PRIMER_PAIR_48_PENALTY=3.008844
PRIMER_LEFT_48_PENALTY=0.801088
PRIMER_RIGHT_48_PENALTY=2.207756
PRIMER_LEFT_48_SEQUENCE=TGCAGCTGTTTACCCATTGT
PRIMER_RIGHT_48_SEQUENCE=AATCAAGCAAGGGATCTCTCAA
PRIMER_LEFT_48=591,20
PRIMER_RIGHT_48=1280,22
PRIMER_LEFT_48_TM=59.199
PRIMER_RIGHT_48_TM=60.208
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=690
PRIMER_PAIR_49_PENALTY=3.033886
PRIMER_LEFT_49_PENALTY=1.548938
PRIMER_RIGHT_49_PENALTY=1.484948
PRIMER_LEFT_49_SEQUENCE=CAACAATGCAGCTGTTTACCC
PRIMER_RIGHT_49_SEQUENCE=TCAAGCAAGGGATCTCTCAA
PRIMER_LEFT_49=585,21
PRIMER_RIGHT_49=1278,20
PRIMER_LEFT_49_TM=60.549
PRIMER_RIGHT_49_TM=58.515
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.4000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=694
=
SEQUENCE_ID=MAGIC.287_4_4481251_4481299_mt-0_rsch-4_31871_25702_TRUE
SEQUENCE_TARGET=950,148
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAANNNNTNANANNATCACATACCCGAGTTAACGGTTGTTAATGTCTTTTNATCNNAATTTCTCGTAAAACTGTATTTTACTCGTAAATAGTTATNTCNGTTAAAATATTGACGGTTANNTTTTTACATTCGTNTNTTTNANNGTANANAGNTGTGTCTGTTAAAATATTAACGGTTATTNTTTTATATTCGTGTCTTTTNTCGTAACTTTTGGTTAACATTNANNNNNNNNNNNNNNNNNNNNNTTTNTATCGCAACTTCTCGTAAAACTGTATTTTTATCGTAAACANTTGTNTCTNTTAAAATATTNACGNNTNACTTTTTNNCATTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTTTNTGTAAAAATANNNNNNNNNNNNNNNNNNNNNNNTGTGTCTCTTTATTNNAACTTTNCGTTATAATATTGACAGTTANTTTTNNTTTTTTGTGTCTGTTTATNNTATNTTTCCTTATGAATAGTTTTCTACTTAAATATTTATAACTGTTGAANTCTATATATTCGTCTTTCTTCTTANGTAATTNTANNNNNTTTGTTCTNTNNNNNNNNTTNTTTNTTTNATGATNAATNNNAAANNAATGTCTTTNGCATTTNTNANCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTAAAATGTTGACGGTTNTTTTTTTGTTCATGTCTTTTCATCGTAANTTTNCGCTAACNATGANNNTTANTTTTTCCGNTACTANATTTTTATCGCAACTTTTTGTTTNTATCTTTGTCATTTTANTATTNACGGTTATTTTTNNNTTNTGTTTNCTNNNNNNNNNNTTTNNTTNAATNNTTTACAATTNCNNNNNNNNNNNNNNNNNTTTCATCGTAACTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACANNTTTTTNTNNNTNTCTTTTNANCGTAACTTTCNGNNNTTATTGACGGTTANTTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17437846_17437857_can-0_hi-0_22772_6491_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=AGACNATCATCACGGAATACTCGGTAGCGGGAACCAACGGCTTGAATCGACTGAATAGGATCTCCACGGCGTTTAGCTAAGTAAATAGCTCGCCGCTGTGCTCTTTTCCGAGCAGCGTCCATCATATCGTGAACCTTCTTCTCCGATCTTCTCGCTTCGTGCCACATCTTGTCCTTGCTCTCTAAGCTCAGAAGAAACCCTAATTAGCTCTTCGAATTGGGAAAATTTTCAGGCGAATTTTGGCGATAATTTTATATTTCCGACGACTAGTTTACAGNGAATTTGGACCGTCCGATGTAAAGCGAAAATAGATCTAGGTTTTCCANGTGTCCNCTATTTTAATGAAACCTTCTGATTCNTGTAGAAGNTTTACTCAATTTAATATTTTTTAGTATGTAGTTNTGTGTGTGTGTGTGTGTGTGNTTTTATGGCTCCACACCAACTTTTAAAATGGTAGAAGCATGTTGCATGTGATCGAGTAAAAAGCCAATANTGAGATTCAGAAAAATAAAAATTACTTATATAGTTTTTTAGAGAAAAAATTGTATTTTGTTTAAAGCCTTAATCCGGTTGTTGAAAGAGCTGTGTCACGAGTTAAAAATATTTTCTTTTCATTTTTTAAGTAATTAGTTTATAATGCAAAAATGGTTTTTATTTATTTGTCTTCGCTTATAGAACTGCAAATTGAGAGANAAAAAAATGAATTAGTGGTGGTGACCAAACATTCAGGAAGCTGTGATTGATCATTTGTTTTTGAGGTGAGTGTAGTGGCAACGTATGACGTTAACATATGGCGTACATAATAATTACATGAACTTAATCATAATAATCATATTGCATTTAATTCNTATATCATATCCCATTAGTTGGACCACTTGATTTGAGGTCATGAGAAGAACATTNNTNTTTTTTTTAGTTTGAATCGGAGTGATCACTAAAAACTAGATACTGAAAATTTTCAAACTAAAATCATATTAATCTTCAAAAAATGTGAAATCTNNAAAAANANAANATTTTAACGCGTTCATTNNNNNNNNGTAGCCAAGTATTGTTAAAGTAGTAGTAAAAGAAGTTTAGCTTTAAGTGATATAATTTGACACAANTCCTACTTAGATATGGATAATAGGATATAGCTTCATGTATATTTTTATNGTTGCTTCTGTAACCCCAAAATGTGTTGATATAAGCATTTGAATATTCGTATGTATAATGTTTTNTTTTCACCGTAAAACATATTACAATGTTAGTTTATATTGGATTTTGAATGTGTTTATGAACAGTTTTTGTCGACTCAAAAGTTAAGATGAGAATATGGAAGAAAGTAAAGTTTAAAAGTCATGATGGNAACAAGGAATGGAACTCAAACATTCTAATACTCAACAAACGCAATTATATTANTACCATGACTCATCTTTCAAGTTCCATCAAAAAGATTCGTGGAAAATAATAGACTTACGTTTCAAATCCATGTTTCTTTCTTTATAACNAAAAAAATGGATGTTTCTTGACGCGTGTCGAGAGTACTCACCATTANTCTGACTTCAGTGAGTTTGGTCAAGTGGTCNTTTTTTTTCTCATGTCACCAAAGGTCCAAACCCTAGAAATTAGTTCGAACTTTNCATAGAAGAACTGAATAAATGGTCCAAAATTGTTTTAAAAAGGACCTAAGCCATTAGTTCATTGAATTCGAGTTAATGGGTGAAGATTTTTATGATAACGAAAGTCGGAGTAATTATGCTTTTGGTCCGATAGTTTTCTAATTTGTTTTCTTTCCNTNTTTTTTTTTTCAAATACTACATACTATATAAGATAGTGGNTTGTGTTAATGTCATCGATGTGTTACCATCCGCATTATATTAATTATTTATCCCAACATAAAGTCAGAATCTGTAATTTCTTTGTTATAAAATACAGTAAATGGTTCCGTTTAAGCTGTTAGATGATTTTTGAGTAAAANCTAATGTNNNNNAAANAAAAAAAAAACAATGTAGTTCATAATACATGCATGTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.133805
PRIMER_LEFT_0_PENALTY=0.431057
PRIMER_RIGHT_0_PENALTY=0.702747
PRIMER_LEFT_0_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_0_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_0=557,20
PRIMER_RIGHT_0=1515,20
PRIMER_LEFT_0_TM=59.569
PRIMER_RIGHT_0_TM=59.297
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=959
PRIMER_PAIR_1_PENALTY=1.448745
PRIMER_LEFT_1_PENALTY=0.431057
PRIMER_RIGHT_1_PENALTY=1.017688
PRIMER_LEFT_1_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_1_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_1=557,20
PRIMER_RIGHT_1=1174,21
PRIMER_LEFT_1_TM=59.569
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=618
PRIMER_PAIR_2_PENALTY=1.462828
PRIMER_LEFT_2_PENALTY=0.431057
PRIMER_RIGHT_2_PENALTY=1.031770
PRIMER_LEFT_2_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_2_SEQUENCE=CGCGTCAAGAAACATCCATT
PRIMER_LEFT_2=557,20
PRIMER_RIGHT_2=1511,20
PRIMER_LEFT_2_TM=59.569
PRIMER_RIGHT_2_TM=61.032
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=955
PRIMER_PAIR_3_PENALTY=1.538510
PRIMER_LEFT_3_PENALTY=0.431057
PRIMER_RIGHT_3_PENALTY=1.107452
PRIMER_LEFT_3_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_3_SEQUENCE=ACACGCGTCAAGAAACATCC
PRIMER_LEFT_3=557,20
PRIMER_RIGHT_3=1514,20
PRIMER_LEFT_3_TM=59.569
PRIMER_RIGHT_3_TM=61.107
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=958
PRIMER_PAIR_4_PENALTY=1.564099
PRIMER_LEFT_4_PENALTY=0.546410
PRIMER_RIGHT_4_PENALTY=1.017688
PRIMER_LEFT_4_SEQUENCE=ATGGCTCCACACCAACTTTT
PRIMER_RIGHT_4_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_4=427,20
PRIMER_RIGHT_4=1174,21
PRIMER_LEFT_4_TM=59.454
PRIMER_RIGHT_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=748
PRIMER_PAIR_5_PENALTY=1.615313
PRIMER_LEFT_5_PENALTY=0.912566
PRIMER_RIGHT_5_PENALTY=0.702747
PRIMER_LEFT_5_SEQUENCE=AAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_5_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_5=556,20
PRIMER_RIGHT_5=1515,20
PRIMER_LEFT_5_TM=59.087
PRIMER_RIGHT_5_TM=59.297
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=960
PRIMER_PAIR_6_PENALTY=1.635153
PRIMER_LEFT_6_PENALTY=1.458762
PRIMER_RIGHT_6_PENALTY=0.176390
PRIMER_LEFT_6_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_6_SEQUENCE=TAGGGTTTGGACCTTTGGTG
PRIMER_LEFT_6=866,20
PRIMER_RIGHT_6=1599,20
PRIMER_LEFT_6_TM=58.541
PRIMER_RIGHT_6_TM=59.824
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=734
PRIMER_PAIR_7_PENALTY=1.737283
PRIMER_LEFT_7_PENALTY=1.560893
PRIMER_RIGHT_7_PENALTY=0.176390
PRIMER_LEFT_7_SEQUENCE=GTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_7_SEQUENCE=TAGGGTTTGGACCTTTGGTG
PRIMER_LEFT_7=865,21
PRIMER_RIGHT_7=1599,20
PRIMER_LEFT_7_TM=59.439
PRIMER_RIGHT_7_TM=59.824
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=735
PRIMER_PAIR_8_PENALTY=1.885470
PRIMER_LEFT_8_PENALTY=0.431057
PRIMER_RIGHT_8_PENALTY=1.454413
PRIMER_LEFT_8_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_8_SEQUENCE=CCACGAATCTTTTTGATGGA
PRIMER_LEFT_8=557,20
PRIMER_RIGHT_8=1439,20
PRIMER_LEFT_8_TM=59.569
PRIMER_RIGHT_8_TM=58.546
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=883
PRIMER_PAIR_9_PENALTY=1.930254
PRIMER_LEFT_9_PENALTY=0.912566
PRIMER_RIGHT_9_PENALTY=1.017688
PRIMER_LEFT_9_SEQUENCE=AAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_9_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_9=556,20
PRIMER_RIGHT_9=1174,21
PRIMER_LEFT_9_TM=59.087
PRIMER_RIGHT_9_TM=59.982
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=619
PRIMER_PAIR_10_PENALTY=1.939245
PRIMER_LEFT_10_PENALTY=1.458762
PRIMER_RIGHT_10_PENALTY=0.480482
PRIMER_LEFT_10_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_10_SEQUENCE=TGGACCTTTGGTGACATGAG
PRIMER_LEFT_10=866,20
PRIMER_RIGHT_10=1592,20
PRIMER_LEFT_10_TM=58.541
PRIMER_RIGHT_10_TM=59.520
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=727
PRIMER_PAIR_11_PENALTY=1.944336
PRIMER_LEFT_11_PENALTY=0.912566
PRIMER_RIGHT_11_PENALTY=1.031770
PRIMER_LEFT_11_SEQUENCE=AAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_11_SEQUENCE=CGCGTCAAGAAACATCCATT
PRIMER_LEFT_11=556,20
PRIMER_RIGHT_11=1511,20
PRIMER_LEFT_11_TM=59.087
PRIMER_RIGHT_11_TM=61.032
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=956
PRIMER_PAIR_12_PENALTY=2.041375
PRIMER_LEFT_12_PENALTY=1.560893
PRIMER_RIGHT_12_PENALTY=0.480482
PRIMER_LEFT_12_SEQUENCE=GTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_12_SEQUENCE=TGGACCTTTGGTGACATGAG
PRIMER_LEFT_12=865,21
PRIMER_RIGHT_12=1592,20
PRIMER_LEFT_12_TM=59.439
PRIMER_RIGHT_12_TM=59.520
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=728
PRIMER_PAIR_13_PENALTY=2.052689
PRIMER_LEFT_13_PENALTY=1.349942
PRIMER_RIGHT_13_PENALTY=0.702747
PRIMER_LEFT_13_SEQUENCE=AAAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_13_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_13=555,21
PRIMER_RIGHT_13=1515,20
PRIMER_LEFT_13_TM=60.350
PRIMER_RIGHT_13_TM=59.297
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=961
PRIMER_PAIR_14_PENALTY=2.161510
PRIMER_LEFT_14_PENALTY=1.458762
PRIMER_RIGHT_14_PENALTY=0.702747
PRIMER_LEFT_14_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_14_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_14=866,20
PRIMER_RIGHT_14=1515,20
PRIMER_LEFT_14_TM=58.541
PRIMER_RIGHT_14_TM=59.297
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.3000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=650
PRIMER_PAIR_15_PENALTY=2.164045
PRIMER_LEFT_15_PENALTY=0.431057
PRIMER_RIGHT_15_PENALTY=1.732988
PRIMER_LEFT_15_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_15_SEQUENCE=TTTTGGGGTTACAGAAGCAA
PRIMER_LEFT_15=557,20
PRIMER_RIGHT_15=1172,20
PRIMER_LEFT_15_TM=59.569
PRIMER_RIGHT_15_TM=58.267
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=616
PRIMER_PAIR_16_PENALTY=2.263640
PRIMER_LEFT_16_PENALTY=1.560893
PRIMER_RIGHT_16_PENALTY=0.702747
PRIMER_LEFT_16_SEQUENCE=GTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_16_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_16=865,21
PRIMER_RIGHT_16=1515,20
PRIMER_LEFT_16_TM=59.439
PRIMER_RIGHT_16_TM=59.297
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=651
PRIMER_PAIR_17_PENALTY=2.264856
PRIMER_LEFT_17_PENALTY=2.088466
PRIMER_RIGHT_17_PENALTY=0.176390
PRIMER_LEFT_17_SEQUENCE=CAAACATTCAGGAAGCTGTGA
PRIMER_RIGHT_17_SEQUENCE=TAGGGTTTGGACCTTTGGTG
PRIMER_LEFT_17=718,21
PRIMER_RIGHT_17=1599,20
PRIMER_LEFT_17_TM=58.912
PRIMER_RIGHT_17_TM=59.824
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=882
PRIMER_PAIR_18_PENALTY=2.279398
PRIMER_LEFT_18_PENALTY=0.546410
PRIMER_RIGHT_18_PENALTY=1.732988
PRIMER_LEFT_18_SEQUENCE=ATGGCTCCACACCAACTTTT
PRIMER_RIGHT_18_SEQUENCE=TTTTGGGGTTACAGAAGCAA
PRIMER_LEFT_18=427,20
PRIMER_RIGHT_18=1172,20
PRIMER_LEFT_18_TM=59.454
PRIMER_RIGHT_18_TM=58.267
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=746
PRIMER_PAIR_19_PENALTY=2.314422
PRIMER_LEFT_19_PENALTY=2.138031
PRIMER_RIGHT_19_PENALTY=0.176390
PRIMER_LEFT_19_SEQUENCE=CATTCAGGAAGCTGTGATTGA
PRIMER_RIGHT_19_SEQUENCE=TAGGGTTTGGACCTTTGGTG
PRIMER_LEFT_19=722,21
PRIMER_RIGHT_19=1599,20
PRIMER_LEFT_19_TM=58.862
PRIMER_RIGHT_19_TM=59.824
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=878
PRIMER_PAIR_20_PENALTY=2.366979
PRIMER_LEFT_20_PENALTY=0.912566
PRIMER_RIGHT_20_PENALTY=1.454413
PRIMER_LEFT_20_SEQUENCE=AAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_20_SEQUENCE=CCACGAATCTTTTTGATGGA
PRIMER_LEFT_20=556,20
PRIMER_RIGHT_20=1439,20
PRIMER_LEFT_20_TM=59.087
PRIMER_RIGHT_20_TM=58.546
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=884
PRIMER_PAIR_21_PENALTY=2.367630
PRIMER_LEFT_21_PENALTY=1.349942
PRIMER_RIGHT_21_PENALTY=1.017688
PRIMER_LEFT_21_SEQUENCE=AAAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_21_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_21=555,21
PRIMER_RIGHT_21=1174,21
PRIMER_LEFT_21_TM=60.350
PRIMER_RIGHT_21_TM=59.982
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=620
PRIMER_PAIR_22_PENALTY=2.381712
PRIMER_LEFT_22_PENALTY=1.349942
PRIMER_RIGHT_22_PENALTY=1.031770
PRIMER_LEFT_22_SEQUENCE=AAAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_22_SEQUENCE=CGCGTCAAGAAACATCCATT
PRIMER_LEFT_22=555,21
PRIMER_RIGHT_22=1511,20
PRIMER_LEFT_22_TM=60.350
PRIMER_RIGHT_22_TM=61.032
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=957
PRIMER_PAIR_23_PENALTY=2.434279
PRIMER_LEFT_23_PENALTY=1.458762
PRIMER_RIGHT_23_PENALTY=0.975516
PRIMER_LEFT_23_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_23_SEQUENCE=TCTAGGGTTTGGACCTTTGG
PRIMER_LEFT_23=866,20
PRIMER_RIGHT_23=1601,20
PRIMER_LEFT_23_TM=58.541
PRIMER_RIGHT_23_TM=59.024
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=736
PRIMER_PAIR_24_PENALTY=2.436094
PRIMER_LEFT_24_PENALTY=2.259704
PRIMER_RIGHT_24_PENALTY=0.176390
PRIMER_LEFT_24_SEQUENCE=ACATTCAGGAAGCTGTGATTGA
PRIMER_RIGHT_24_SEQUENCE=TAGGGTTTGGACCTTTGGTG
PRIMER_LEFT_24=721,22
PRIMER_RIGHT_24=1599,20
PRIMER_LEFT_24_TM=59.740
PRIMER_RIGHT_24_TM=59.824
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=879
PRIMER_PAIR_25_PENALTY=2.490533
PRIMER_LEFT_25_PENALTY=1.458762
PRIMER_RIGHT_25_PENALTY=1.031770
PRIMER_LEFT_25_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_25_SEQUENCE=CGCGTCAAGAAACATCCATT
PRIMER_LEFT_25=866,20
PRIMER_RIGHT_25=1511,20
PRIMER_LEFT_25_TM=58.541
PRIMER_RIGHT_25_TM=61.032
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=646
PRIMER_PAIR_26_PENALTY=2.500507
PRIMER_LEFT_26_PENALTY=1.458762
PRIMER_RIGHT_26_PENALTY=1.041745
PRIMER_LEFT_26_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_26_SEQUENCE=GGACCTTTGGTGACATGAGAA
PRIMER_LEFT_26=866,20
PRIMER_RIGHT_26=1591,21
PRIMER_LEFT_26_TM=58.541
PRIMER_RIGHT_26_TM=59.958
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=726
PRIMER_PAIR_27_PENALTY=2.532890
PRIMER_LEFT_27_PENALTY=1.515201
PRIMER_RIGHT_27_PENALTY=1.017688
PRIMER_LEFT_27_SEQUENCE=TATGGCTCCACACCAACTTTT
PRIMER_RIGHT_27_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_27=426,21
PRIMER_RIGHT_27=1174,21
PRIMER_LEFT_27_TM=59.485
PRIMER_RIGHT_27_TM=59.982
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.3000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=749
PRIMER_PAIR_28_PENALTY=2.532890
PRIMER_LEFT_28_PENALTY=1.515201
PRIMER_RIGHT_28_PENALTY=1.017688
PRIMER_LEFT_28_SEQUENCE=TTTTATGGCTCCACACCAACT
PRIMER_RIGHT_28_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_28=423,21
PRIMER_RIGHT_28=1174,21
PRIMER_LEFT_28_TM=59.485
PRIMER_RIGHT_28_TM=59.982
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=752
PRIMER_PAIR_29_PENALTY=2.536409
PRIMER_LEFT_29_PENALTY=1.560893
PRIMER_RIGHT_29_PENALTY=0.975516
PRIMER_LEFT_29_SEQUENCE=GTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_29_SEQUENCE=TCTAGGGTTTGGACCTTTGG
PRIMER_LEFT_29=865,21
PRIMER_RIGHT_29=1601,20
PRIMER_LEFT_29_TM=59.439
PRIMER_RIGHT_29_TM=59.024
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=737
PRIMER_PAIR_30_PENALTY=2.537807
PRIMER_LEFT_30_PENALTY=1.835060
PRIMER_RIGHT_30_PENALTY=0.702747
PRIMER_LEFT_30_SEQUENCE=AAGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_30_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_30=556,21
PRIMER_RIGHT_30=1515,20
PRIMER_LEFT_30_TM=60.835
PRIMER_RIGHT_30_TM=59.297
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.3000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=960
PRIMER_PAIR_31_PENALTY=2.542043
PRIMER_LEFT_31_PENALTY=1.524354
PRIMER_RIGHT_31_PENALTY=1.017688
PRIMER_LEFT_31_SEQUENCE=TTTTATGGCTCCACACCAAC
PRIMER_RIGHT_31_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_31=423,20
PRIMER_RIGHT_31=1174,21
PRIMER_LEFT_31_TM=58.476
PRIMER_RIGHT_31_TM=59.982
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=752
PRIMER_PAIR_32_PENALTY=2.566215
PRIMER_LEFT_32_PENALTY=1.458762
PRIMER_RIGHT_32_PENALTY=1.107452
PRIMER_LEFT_32_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_32_SEQUENCE=ACACGCGTCAAGAAACATCC
PRIMER_LEFT_32=866,20
PRIMER_RIGHT_32=1514,20
PRIMER_LEFT_32_TM=58.541
PRIMER_RIGHT_32_TM=61.107
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=649
PRIMER_PAIR_33_PENALTY=2.568948
PRIMER_LEFT_33_PENALTY=2.088466
PRIMER_RIGHT_33_PENALTY=0.480482
PRIMER_LEFT_33_SEQUENCE=CAAACATTCAGGAAGCTGTGA
PRIMER_RIGHT_33_SEQUENCE=TGGACCTTTGGTGACATGAG
PRIMER_LEFT_33=718,21
PRIMER_RIGHT_33=1592,20
PRIMER_LEFT_33_TM=58.912
PRIMER_RIGHT_33_TM=59.520
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=875
PRIMER_PAIR_34_PENALTY=2.571063
PRIMER_LEFT_34_PENALTY=2.394672
PRIMER_RIGHT_34_PENALTY=0.176390
PRIMER_LEFT_34_SEQUENCE=AGTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_34_SEQUENCE=TAGGGTTTGGACCTTTGGTG
PRIMER_LEFT_34=864,22
PRIMER_RIGHT_34=1599,20
PRIMER_LEFT_34_TM=60.395
PRIMER_RIGHT_34_TM=59.824
PRIMER_LEFT_34_GC_PERCENT=45.455
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=736
PRIMER_PAIR_35_PENALTY=2.592663
PRIMER_LEFT_35_PENALTY=1.560893
PRIMER_RIGHT_35_PENALTY=1.031770
PRIMER_LEFT_35_SEQUENCE=GTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_35_SEQUENCE=CGCGTCAAGAAACATCCATT
PRIMER_LEFT_35=865,21
PRIMER_RIGHT_35=1511,20
PRIMER_LEFT_35_TM=59.439
PRIMER_RIGHT_35_TM=61.032
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.4000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=647
PRIMER_PAIR_36_PENALTY=2.602638
PRIMER_LEFT_36_PENALTY=1.560893
PRIMER_RIGHT_36_PENALTY=1.041745
PRIMER_LEFT_36_SEQUENCE=GTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_36_SEQUENCE=GGACCTTTGGTGACATGAGAA
PRIMER_LEFT_36=865,21
PRIMER_RIGHT_36=1591,21
PRIMER_LEFT_36_TM=59.439
PRIMER_RIGHT_36_TM=59.958
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=727
PRIMER_PAIR_37_PENALTY=2.618514
PRIMER_LEFT_37_PENALTY=2.138031
PRIMER_RIGHT_37_PENALTY=0.480482
PRIMER_LEFT_37_SEQUENCE=CATTCAGGAAGCTGTGATTGA
PRIMER_RIGHT_37_SEQUENCE=TGGACCTTTGGTGACATGAG
PRIMER_LEFT_37=722,21
PRIMER_RIGHT_37=1592,20
PRIMER_LEFT_37_TM=58.862
PRIMER_RIGHT_37_TM=59.520
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=871
PRIMER_PAIR_38_PENALTY=2.645554
PRIMER_LEFT_38_PENALTY=0.912566
PRIMER_RIGHT_38_PENALTY=1.732988
PRIMER_LEFT_38_SEQUENCE=AAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_38_SEQUENCE=TTTTGGGGTTACAGAAGCAA
PRIMER_LEFT_38=556,20
PRIMER_RIGHT_38=1172,20
PRIMER_LEFT_38_TM=59.087
PRIMER_RIGHT_38_TM=58.267
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=617
PRIMER_PAIR_39_PENALTY=2.668256
PRIMER_LEFT_39_PENALTY=0.431057
PRIMER_RIGHT_39_PENALTY=2.237199
PRIMER_LEFT_39_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_39_SEQUENCE=CAACACATTTTGGGGTTACAGA
PRIMER_LEFT_39=557,20
PRIMER_RIGHT_39=1179,22
PRIMER_LEFT_39_TM=59.569
PRIMER_RIGHT_39_TM=59.763
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=623
PRIMER_PAIR_40_PENALTY=2.668256
PRIMER_LEFT_40_PENALTY=0.431057
PRIMER_RIGHT_40_PENALTY=2.237199
PRIMER_LEFT_40_SEQUENCE=AGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_40_SEQUENCE=TCAACACATTTTGGGGTTACAG
PRIMER_LEFT_40=557,20
PRIMER_RIGHT_40=1180,22
PRIMER_LEFT_40_TM=59.569
PRIMER_RIGHT_40_TM=59.763
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=5.7000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=624
PRIMER_PAIR_41_PENALTY=2.668345
PRIMER_LEFT_41_PENALTY=1.560893
PRIMER_RIGHT_41_PENALTY=1.107452
PRIMER_LEFT_41_SEQUENCE=GTTGGACCACTTGATTTGAGG
PRIMER_RIGHT_41_SEQUENCE=ACACGCGTCAAGAAACATCC
PRIMER_LEFT_41=865,21
PRIMER_RIGHT_41=1514,20
PRIMER_LEFT_41_TM=59.439
PRIMER_RIGHT_41_TM=61.107
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=650
PRIMER_PAIR_42_PENALTY=2.740186
PRIMER_LEFT_42_PENALTY=2.259704
PRIMER_RIGHT_42_PENALTY=0.480482
PRIMER_LEFT_42_SEQUENCE=ACATTCAGGAAGCTGTGATTGA
PRIMER_RIGHT_42_SEQUENCE=TGGACCTTTGGTGACATGAG
PRIMER_LEFT_42=721,22
PRIMER_RIGHT_42=1592,20
PRIMER_LEFT_42_TM=59.740
PRIMER_RIGHT_42_TM=59.520
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=6.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=872
PRIMER_PAIR_43_PENALTY=2.783609
PRIMER_LEFT_43_PENALTY=0.546410
PRIMER_RIGHT_43_PENALTY=2.237199
PRIMER_LEFT_43_SEQUENCE=ATGGCTCCACACCAACTTTT
PRIMER_RIGHT_43_SEQUENCE=CAACACATTTTGGGGTTACAGA
PRIMER_LEFT_43=427,20
PRIMER_RIGHT_43=1179,22
PRIMER_LEFT_43_TM=59.454
PRIMER_RIGHT_43_TM=59.763
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=753
PRIMER_PAIR_44_PENALTY=2.783609
PRIMER_LEFT_44_PENALTY=0.546410
PRIMER_RIGHT_44_PENALTY=2.237199
PRIMER_LEFT_44_SEQUENCE=ATGGCTCCACACCAACTTTT
PRIMER_RIGHT_44_SEQUENCE=TCAACACATTTTGGGGTTACAG
PRIMER_LEFT_44=427,20
PRIMER_RIGHT_44=1180,22
PRIMER_LEFT_44_TM=59.454
PRIMER_RIGHT_44_TM=59.763
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=5.7000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=754
PRIMER_PAIR_45_PENALTY=2.789302
PRIMER_LEFT_45_PENALTY=1.458762
PRIMER_RIGHT_45_PENALTY=1.330540
PRIMER_LEFT_45_SEQUENCE=TTGGACCACTTGATTTGAGG
PRIMER_RIGHT_45_SEQUENCE=TTCTAGGGTTTGGACCTTTGG
PRIMER_LEFT_45=866,20
PRIMER_RIGHT_45=1602,21
PRIMER_LEFT_45_TM=58.541
PRIMER_RIGHT_45_TM=60.331
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=737
PRIMER_PAIR_46_PENALTY=2.791213
PRIMER_LEFT_46_PENALTY=2.088466
PRIMER_RIGHT_46_PENALTY=0.702747
PRIMER_LEFT_46_SEQUENCE=CAAACATTCAGGAAGCTGTGA
PRIMER_RIGHT_46_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_46=718,21
PRIMER_RIGHT_46=1515,20
PRIMER_LEFT_46_TM=58.912
PRIMER_RIGHT_46_TM=59.297
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.3000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=798
PRIMER_PAIR_47_PENALTY=2.804355
PRIMER_LEFT_47_PENALTY=1.349942
PRIMER_RIGHT_47_PENALTY=1.454413
PRIMER_LEFT_47_SEQUENCE=AAAGCCTTAATCCGGTTGTTG
PRIMER_RIGHT_47_SEQUENCE=CCACGAATCTTTTTGATGGA
PRIMER_LEFT_47=555,21
PRIMER_RIGHT_47=1439,20
PRIMER_LEFT_47_TM=60.350
PRIMER_RIGHT_47_TM=58.546
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.1000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=885
PRIMER_PAIR_48_PENALTY=2.840779
PRIMER_LEFT_48_PENALTY=2.138031
PRIMER_RIGHT_48_PENALTY=0.702747
PRIMER_LEFT_48_SEQUENCE=CATTCAGGAAGCTGTGATTGA
PRIMER_RIGHT_48_SEQUENCE=GACACGCGTCAAGAAACATC
PRIMER_LEFT_48=722,21
PRIMER_RIGHT_48=1515,20
PRIMER_LEFT_48_TM=58.862
PRIMER_RIGHT_48_TM=59.297
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=6.3000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=794
PRIMER_PAIR_49_PENALTY=2.852748
PRIMER_LEFT_49_PENALTY=1.835060
PRIMER_RIGHT_49_PENALTY=1.017688
PRIMER_LEFT_49_SEQUENCE=AAGCCTTAATCCGGTTGTTGA
PRIMER_RIGHT_49_SEQUENCE=CATTTTGGGGTTACAGAAGCA
PRIMER_LEFT_49=556,21
PRIMER_RIGHT_49=1174,21
PRIMER_LEFT_49_TM=60.835
PRIMER_RIGHT_49_TM=59.982
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=619
=
SEQUENCE_ID=MAGIC.287_3_11956787_11956866_ct-1_col-0_1475368_78_TRUE
SEQUENCE_TARGET=950,179
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACGTTCTATAATTTNTATNNCTTTNAATAATTTATTNAATTAATACTTAAATAATAAAGGGTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTNAANNANATAAATGGTATGTCTTTCACTTTGNACGGAGTATCCCNATGTGGGGTACATAAATATTAAGAGAAAAAAAGAGATCAAGCTTACTAGTTTAAGCGTTAATAATTATACTGACTGACCAATCTACTTGTTCATGTAGCTCTAATGTTATTAATTANTATATAGTCTTAGATTGATCTAATTAATGTCTGTGTATGATGTCACTTTGTGATGATGGAAGTCATGAGAGCTTCCATTACTTAACTCTCTCTCTCTCTNTCTATNTATNTTTACGGTCATGTATAGTGTAATCANNAATAACAAGAAAAAGCCAAGTTCAAAGCACNATACTATTAGTTAAAGACAAATGCGATAATGATACGATTCTGAGCATTACTTGAGTCNCAATATATAGTCACACCCTCATCAAATTCTTTNTATCTTACCAACTTCTNAAAAAAAANNAAAAANNNAAAAGACAAGTAAATACATANATAAATATACACNCACACACACACACATATATTGATATACTTATAATNATAAATCATGGTAAAGTTTGANACAAAACTATGGAAGGCTGTGCTTATGATGTCCATGATCAACATTGGTTTAAGTGTTGTAAACGTTATGTTCAAGAAGATGATTGATGAAGGACTTAACCGTATGGTCGCGACCACTTACCGACTTGCTGTAGGAACTCTGTTCTTNATACCATTCGCGATTTTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNTTNNNNNNNNTNTNNNNNGNTNNNTGTNTTNTTNNTTNTNNNNNTTNNANTTNTNNTTNAANTNTNNNNTTNAATNATAANNGGATGTCTTGTTTATACNANTNTCTAGTTGAATGTTGTTTTCAAACTCTTTTCTCCTGTGAATATGTNATTATATATTCGTATTCCATATGAAAGATTTTGNNNNNNNNNNNNNTTGTTCTTTTTTTNNNNNTCTCTTTTNGTAGACATAACAGGCCAAAACTCACGGGTAGGATCTTGTGTTCACTCTTCTTTAGCGCTCTTCTGGGGTATGTTCATTTTATTAGACTGTTCANTTATAATAAATGTTTACAAATAAGTTTTGAGTTGTTTNTTNTGATCTTGATCGTTNNTATATTTGCAGGACAAGTTTGGTGCAATACTTCTTTCTTATTGGACTAGAGTATACATCTTCAACNTTTTCCTTAGCNTTTAGCAACATGGTTCCTTCNGTTACNTTTGCNTTGGCTCTCGTCTTTAGGTANTNAAAAATCGATCAAACNCATTTGNATTCTTCATATATATNCTCTAGTATTATCNNAATNNTTNATAAACTANANGANTAGGCAAGAGACGCTGAACATCAAGAGCAACGTAGGAAGAGCCAAACTGCTAGGCACAATGATATGTATTTGTGGAGCTTTAGTGCTTACGCTTTATAAAGGAACCGCTTTAAGTCGAGAACANAGTACTCATATGNAAACACATACAAGAACTGATTCCACCGGTGCAATGACNCAAAAGTGGGCAATGGGTTCGATCATGCTGGTTATATCNATCATNATATGGTCATCTTGGTTCATTGTTCAAGCTAAAATAAGCNGANTTTATCCNTGCCAATACACGAGTACCACGATCCTCTCTTTCTTTGGCGTGATNCAATCTGCTTTGTTGAGTTTGATCTCGGAGAGGAGCACNTCTATGTGGGTCGTTAAAGACAAGTTCCAAGTTTTAGCTTTACTTTATTCGGTGAGTNA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.689693
PRIMER_LEFT_0_PENALTY=1.674441
PRIMER_RIGHT_0_PENALTY=0.015253
PRIMER_LEFT_0_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_0_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_0=864,20
PRIMER_RIGHT_0=1709,20
PRIMER_LEFT_0_TM=58.326
PRIMER_RIGHT_0_TM=60.015
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=846
PRIMER_PAIR_1_PENALTY=1.694207
PRIMER_LEFT_1_PENALTY=1.674441
PRIMER_RIGHT_1_PENALTY=0.019767
PRIMER_LEFT_1_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_1_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_1=864,20
PRIMER_RIGHT_1=1685,20
PRIMER_LEFT_1_TM=58.326
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=822
PRIMER_PAIR_2_PENALTY=1.699569
PRIMER_LEFT_2_PENALTY=1.674441
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_2_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_2=864,20
PRIMER_RIGHT_2=1373,20
PRIMER_LEFT_2_TM=58.326
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=510
PRIMER_PAIR_3_PENALTY=1.708121
PRIMER_LEFT_3_PENALTY=1.674441
PRIMER_RIGHT_3_PENALTY=0.033680
PRIMER_LEFT_3_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_3_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_3=864,20
PRIMER_RIGHT_3=1829,20
PRIMER_LEFT_3_TM=58.326
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=966
PRIMER_PAIR_4_PENALTY=1.708121
PRIMER_LEFT_4_PENALTY=1.674441
PRIMER_RIGHT_4_PENALTY=0.033680
PRIMER_LEFT_4_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_4_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_4=864,20
PRIMER_RIGHT_4=1830,20
PRIMER_LEFT_4_TM=58.326
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=967
PRIMER_PAIR_5_PENALTY=1.802887
PRIMER_LEFT_5_PENALTY=1.787634
PRIMER_RIGHT_5_PENALTY=0.015253
PRIMER_LEFT_5_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_5_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_5=866,21
PRIMER_RIGHT_5=1709,20
PRIMER_LEFT_5_TM=59.212
PRIMER_RIGHT_5_TM=60.015
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=844
PRIMER_PAIR_6_PENALTY=1.802887
PRIMER_LEFT_6_PENALTY=1.787634
PRIMER_RIGHT_6_PENALTY=0.015253
PRIMER_LEFT_6_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_6_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_6=864,21
PRIMER_RIGHT_6=1709,20
PRIMER_LEFT_6_TM=59.212
PRIMER_RIGHT_6_TM=60.015
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.3000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=846
PRIMER_PAIR_7_PENALTY=1.807401
PRIMER_LEFT_7_PENALTY=1.787634
PRIMER_RIGHT_7_PENALTY=0.019767
PRIMER_LEFT_7_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_7_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_7=866,21
PRIMER_RIGHT_7=1685,20
PRIMER_LEFT_7_TM=59.212
PRIMER_RIGHT_7_TM=60.020
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=820
PRIMER_PAIR_8_PENALTY=1.807401
PRIMER_LEFT_8_PENALTY=1.787634
PRIMER_RIGHT_8_PENALTY=0.019767
PRIMER_LEFT_8_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_8_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_8=864,21
PRIMER_RIGHT_8=1685,20
PRIMER_LEFT_8_TM=59.212
PRIMER_RIGHT_8_TM=60.020
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.3000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=822
PRIMER_PAIR_9_PENALTY=1.812762
PRIMER_LEFT_9_PENALTY=1.787634
PRIMER_RIGHT_9_PENALTY=0.025128
PRIMER_LEFT_9_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_9_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_9=866,21
PRIMER_RIGHT_9=1373,20
PRIMER_LEFT_9_TM=59.212
PRIMER_RIGHT_9_TM=59.975
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=508
PRIMER_PAIR_10_PENALTY=1.812762
PRIMER_LEFT_10_PENALTY=1.787634
PRIMER_RIGHT_10_PENALTY=0.025128
PRIMER_LEFT_10_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_10_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_10=864,21
PRIMER_RIGHT_10=1373,20
PRIMER_LEFT_10_TM=59.212
PRIMER_RIGHT_10_TM=59.975
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.3000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=510
PRIMER_PAIR_11_PENALTY=1.821314
PRIMER_LEFT_11_PENALTY=1.787634
PRIMER_RIGHT_11_PENALTY=0.033680
PRIMER_LEFT_11_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_11_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_11=866,21
PRIMER_RIGHT_11=1829,20
PRIMER_LEFT_11_TM=59.212
PRIMER_RIGHT_11_TM=59.966
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=964
PRIMER_PAIR_12_PENALTY=1.821314
PRIMER_LEFT_12_PENALTY=1.787634
PRIMER_RIGHT_12_PENALTY=0.033680
PRIMER_LEFT_12_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_12_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_12=866,21
PRIMER_RIGHT_12=1830,20
PRIMER_LEFT_12_TM=59.212
PRIMER_RIGHT_12_TM=59.966
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=965
PRIMER_PAIR_13_PENALTY=1.821314
PRIMER_LEFT_13_PENALTY=1.787634
PRIMER_RIGHT_13_PENALTY=0.033680
PRIMER_LEFT_13_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_13_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_13=864,21
PRIMER_RIGHT_13=1829,20
PRIMER_LEFT_13_TM=59.212
PRIMER_RIGHT_13_TM=59.966
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.3000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=966
PRIMER_PAIR_14_PENALTY=1.821314
PRIMER_LEFT_14_PENALTY=1.787634
PRIMER_RIGHT_14_PENALTY=0.033680
PRIMER_LEFT_14_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_14_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_14=864,21
PRIMER_RIGHT_14=1830,20
PRIMER_LEFT_14_TM=59.212
PRIMER_RIGHT_14_TM=59.966
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.3000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=967
PRIMER_PAIR_15_PENALTY=1.828463
PRIMER_LEFT_15_PENALTY=1.674441
PRIMER_RIGHT_15_PENALTY=0.154023
PRIMER_LEFT_15_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_15_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_15=864,20
PRIMER_RIGHT_15=1720,20
PRIMER_LEFT_15_TM=58.326
PRIMER_RIGHT_15_TM=60.154
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=857
PRIMER_PAIR_16_PENALTY=1.940877
PRIMER_LEFT_16_PENALTY=1.674441
PRIMER_RIGHT_16_PENALTY=0.266437
PRIMER_LEFT_16_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_16_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_16=864,20
PRIMER_RIGHT_16=1478,20
PRIMER_LEFT_16_TM=58.326
PRIMER_RIGHT_16_TM=59.734
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=8.8000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=615
PRIMER_PAIR_17_PENALTY=1.941657
PRIMER_LEFT_17_PENALTY=1.787634
PRIMER_RIGHT_17_PENALTY=0.154023
PRIMER_LEFT_17_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_17_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_17=866,21
PRIMER_RIGHT_17=1720,20
PRIMER_LEFT_17_TM=59.212
PRIMER_RIGHT_17_TM=60.154
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=855
PRIMER_PAIR_18_PENALTY=1.941657
PRIMER_LEFT_18_PENALTY=1.787634
PRIMER_RIGHT_18_PENALTY=0.154023
PRIMER_LEFT_18_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_18_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_18=864,21
PRIMER_RIGHT_18=1720,20
PRIMER_LEFT_18_TM=59.212
PRIMER_RIGHT_18_TM=60.154
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.3000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=857
PRIMER_PAIR_19_PENALTY=1.950190
PRIMER_LEFT_19_PENALTY=1.674441
PRIMER_RIGHT_19_PENALTY=0.275749
PRIMER_LEFT_19_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_19_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_19=864,20
PRIMER_RIGHT_19=1370,20
PRIMER_LEFT_19_TM=58.326
PRIMER_RIGHT_19_TM=59.724
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=507
PRIMER_PAIR_20_PENALTY=2.030013
PRIMER_LEFT_20_PENALTY=1.674441
PRIMER_RIGHT_20_PENALTY=0.355573
PRIMER_LEFT_20_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_20_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_20=864,20
PRIMER_RIGHT_20=1721,20
PRIMER_LEFT_20_TM=58.326
PRIMER_RIGHT_20_TM=59.644
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=858
PRIMER_PAIR_21_PENALTY=2.054071
PRIMER_LEFT_21_PENALTY=1.787634
PRIMER_RIGHT_21_PENALTY=0.266437
PRIMER_LEFT_21_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_21_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_21=866,21
PRIMER_RIGHT_21=1478,20
PRIMER_LEFT_21_TM=59.212
PRIMER_RIGHT_21_TM=59.734
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=613
PRIMER_PAIR_22_PENALTY=2.054071
PRIMER_LEFT_22_PENALTY=1.787634
PRIMER_RIGHT_22_PENALTY=0.266437
PRIMER_LEFT_22_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_22_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_22=864,21
PRIMER_RIGHT_22=1478,20
PRIMER_LEFT_22_TM=59.212
PRIMER_RIGHT_22_TM=59.734
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.3000
PRIMER_RIGHT_22_END_STABILITY=8.8000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=615
PRIMER_PAIR_23_PENALTY=2.063383
PRIMER_LEFT_23_PENALTY=1.787634
PRIMER_RIGHT_23_PENALTY=0.275749
PRIMER_LEFT_23_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_23_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_23=866,21
PRIMER_RIGHT_23=1370,20
PRIMER_LEFT_23_TM=59.212
PRIMER_RIGHT_23_TM=59.724
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=505
PRIMER_PAIR_24_PENALTY=2.063383
PRIMER_LEFT_24_PENALTY=1.787634
PRIMER_RIGHT_24_PENALTY=0.275749
PRIMER_LEFT_24_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_24_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_24=864,21
PRIMER_RIGHT_24=1370,20
PRIMER_LEFT_24_TM=59.212
PRIMER_RIGHT_24_TM=59.724
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.3000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=507
PRIMER_PAIR_25_PENALTY=2.078114
PRIMER_LEFT_25_PENALTY=1.674441
PRIMER_RIGHT_25_PENALTY=0.403673
PRIMER_LEFT_25_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_25_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_25=864,20
PRIMER_RIGHT_25=1694,20
PRIMER_LEFT_25_TM=58.326
PRIMER_RIGHT_25_TM=59.596
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=831
PRIMER_PAIR_26_PENALTY=2.143207
PRIMER_LEFT_26_PENALTY=1.787634
PRIMER_RIGHT_26_PENALTY=0.355573
PRIMER_LEFT_26_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_26_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_26=866,21
PRIMER_RIGHT_26=1721,20
PRIMER_LEFT_26_TM=59.212
PRIMER_RIGHT_26_TM=59.644
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=856
PRIMER_PAIR_27_PENALTY=2.143207
PRIMER_LEFT_27_PENALTY=1.787634
PRIMER_RIGHT_27_PENALTY=0.355573
PRIMER_LEFT_27_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_27_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_27=864,21
PRIMER_RIGHT_27=1721,20
PRIMER_LEFT_27_TM=59.212
PRIMER_RIGHT_27_TM=59.644
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.3000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=858
PRIMER_PAIR_28_PENALTY=2.187625
PRIMER_LEFT_28_PENALTY=2.172373
PRIMER_RIGHT_28_PENALTY=0.015253
PRIMER_LEFT_28_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_28_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_28=863,21
PRIMER_RIGHT_28=1709,20
PRIMER_LEFT_28_TM=61.172
PRIMER_RIGHT_28_TM=60.015
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=847
PRIMER_PAIR_29_PENALTY=2.191308
PRIMER_LEFT_29_PENALTY=1.787634
PRIMER_RIGHT_29_PENALTY=0.403673
PRIMER_LEFT_29_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_29_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_29=866,21
PRIMER_RIGHT_29=1694,20
PRIMER_LEFT_29_TM=59.212
PRIMER_RIGHT_29_TM=59.596
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=829
PRIMER_PAIR_30_PENALTY=2.191308
PRIMER_LEFT_30_PENALTY=1.787634
PRIMER_RIGHT_30_PENALTY=0.403673
PRIMER_LEFT_30_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_30_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_30=864,21
PRIMER_RIGHT_30=1694,20
PRIMER_LEFT_30_TM=59.212
PRIMER_RIGHT_30_TM=59.596
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.3000
PRIMER_RIGHT_30_END_STABILITY=9.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=831
PRIMER_PAIR_31_PENALTY=2.192139
PRIMER_LEFT_31_PENALTY=2.172373
PRIMER_RIGHT_31_PENALTY=0.019767
PRIMER_LEFT_31_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_31_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_31=863,21
PRIMER_RIGHT_31=1685,20
PRIMER_LEFT_31_TM=61.172
PRIMER_RIGHT_31_TM=60.020
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=823
PRIMER_PAIR_32_PENALTY=2.197501
PRIMER_LEFT_32_PENALTY=2.172373
PRIMER_RIGHT_32_PENALTY=0.025128
PRIMER_LEFT_32_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_32_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_32=863,21
PRIMER_RIGHT_32=1373,20
PRIMER_LEFT_32_TM=61.172
PRIMER_RIGHT_32_TM=59.975
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=6.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=511
PRIMER_PAIR_33_PENALTY=2.206053
PRIMER_LEFT_33_PENALTY=2.172373
PRIMER_RIGHT_33_PENALTY=0.033680
PRIMER_LEFT_33_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_33_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_33=863,21
PRIMER_RIGHT_33=1829,20
PRIMER_LEFT_33_TM=61.172
PRIMER_RIGHT_33_TM=59.966
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=967
PRIMER_PAIR_34_PENALTY=2.206053
PRIMER_LEFT_34_PENALTY=2.172373
PRIMER_RIGHT_34_PENALTY=0.033680
PRIMER_LEFT_34_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_34_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_34=863,21
PRIMER_RIGHT_34=1830,20
PRIMER_LEFT_34_TM=61.172
PRIMER_RIGHT_34_TM=59.966
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=968
PRIMER_PAIR_35_PENALTY=2.212524
PRIMER_LEFT_35_PENALTY=1.674441
PRIMER_RIGHT_35_PENALTY=0.538083
PRIMER_LEFT_35_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_35_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_35=864,20
PRIMER_RIGHT_35=1708,20
PRIMER_LEFT_35_TM=58.326
PRIMER_RIGHT_35_TM=60.538
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=845
PRIMER_PAIR_36_PENALTY=2.250680
PRIMER_LEFT_36_PENALTY=2.235428
PRIMER_RIGHT_36_PENALTY=0.015253
PRIMER_LEFT_36_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_36_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_36=867,20
PRIMER_RIGHT_36=1709,20
PRIMER_LEFT_36_TM=57.765
PRIMER_RIGHT_36_TM=60.015
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=843
PRIMER_PAIR_37_PENALTY=2.255194
PRIMER_LEFT_37_PENALTY=2.235428
PRIMER_RIGHT_37_PENALTY=0.019767
PRIMER_LEFT_37_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_37_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_37=867,20
PRIMER_RIGHT_37=1685,20
PRIMER_LEFT_37_TM=57.765
PRIMER_RIGHT_37_TM=60.020
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=819
PRIMER_PAIR_38_PENALTY=2.260556
PRIMER_LEFT_38_PENALTY=2.235428
PRIMER_RIGHT_38_PENALTY=0.025128
PRIMER_LEFT_38_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_38_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_38=867,20
PRIMER_RIGHT_38=1373,20
PRIMER_LEFT_38_TM=57.765
PRIMER_RIGHT_38_TM=59.975
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=507
PRIMER_PAIR_39_PENALTY=2.269108
PRIMER_LEFT_39_PENALTY=2.235428
PRIMER_RIGHT_39_PENALTY=0.033680
PRIMER_LEFT_39_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_39_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_39=867,20
PRIMER_RIGHT_39=1829,20
PRIMER_LEFT_39_TM=57.765
PRIMER_RIGHT_39_TM=59.966
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=963
PRIMER_PAIR_40_PENALTY=2.269108
PRIMER_LEFT_40_PENALTY=2.235428
PRIMER_RIGHT_40_PENALTY=0.033680
PRIMER_LEFT_40_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_40_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_40=867,20
PRIMER_RIGHT_40=1830,20
PRIMER_LEFT_40_TM=57.765
PRIMER_RIGHT_40_TM=59.966
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=964
PRIMER_PAIR_41_PENALTY=2.301346
PRIMER_LEFT_41_PENALTY=2.276218
PRIMER_RIGHT_41_PENALTY=0.025128
PRIMER_LEFT_41_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_41_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_41=614,22
PRIMER_RIGHT_41=1373,20
PRIMER_LEFT_41_TM=60.276
PRIMER_RIGHT_41_TM=59.975
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=6.3000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=760
PRIMER_PAIR_42_PENALTY=2.325718
PRIMER_LEFT_42_PENALTY=1.787634
PRIMER_RIGHT_42_PENALTY=0.538083
PRIMER_LEFT_42_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_42_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_42=866,21
PRIMER_RIGHT_42=1708,20
PRIMER_LEFT_42_TM=59.212
PRIMER_RIGHT_42_TM=60.538
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=843
PRIMER_PAIR_43_PENALTY=2.325718
PRIMER_LEFT_43_PENALTY=1.787634
PRIMER_RIGHT_43_PENALTY=0.538083
PRIMER_LEFT_43_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_43_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_43=864,21
PRIMER_RIGHT_43=1708,20
PRIMER_LEFT_43_TM=59.212
PRIMER_RIGHT_43_TM=60.538
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.3000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=845
PRIMER_PAIR_44_PENALTY=2.326395
PRIMER_LEFT_44_PENALTY=2.172373
PRIMER_RIGHT_44_PENALTY=0.154023
PRIMER_LEFT_44_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_44_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_44=863,21
PRIMER_RIGHT_44=1720,20
PRIMER_LEFT_44_TM=61.172
PRIMER_RIGHT_44_TM=60.154
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=858
PRIMER_PAIR_45_PENALTY=2.389450
PRIMER_LEFT_45_PENALTY=2.235428
PRIMER_RIGHT_45_PENALTY=0.154023
PRIMER_LEFT_45_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_45_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_45=867,20
PRIMER_RIGHT_45=1720,20
PRIMER_LEFT_45_TM=57.765
PRIMER_RIGHT_45_TM=60.154
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=854
PRIMER_PAIR_46_PENALTY=2.438809
PRIMER_LEFT_46_PENALTY=2.172373
PRIMER_RIGHT_46_PENALTY=0.266437
PRIMER_LEFT_46_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_46_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_46=863,21
PRIMER_RIGHT_46=1478,20
PRIMER_LEFT_46_TM=61.172
PRIMER_RIGHT_46_TM=59.734
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.8000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=616
PRIMER_PAIR_47_PENALTY=2.448122
PRIMER_LEFT_47_PENALTY=2.172373
PRIMER_RIGHT_47_PENALTY=0.275749
PRIMER_LEFT_47_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_47_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_47=863,21
PRIMER_RIGHT_47=1370,20
PRIMER_LEFT_47_TM=61.172
PRIMER_RIGHT_47_TM=59.724
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=508
PRIMER_PAIR_48_PENALTY=2.452020
PRIMER_LEFT_48_PENALTY=1.674441
PRIMER_RIGHT_48_PENALTY=0.777580
PRIMER_LEFT_48_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_48_SEQUENCE=GCCTAGCAGTTTGGCTCTTC
PRIMER_LEFT_48=864,20
PRIMER_RIGHT_48=1719,20
PRIMER_LEFT_48_TM=58.326
PRIMER_RIGHT_48_TM=59.222
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=856
PRIMER_PAIR_49_PENALTY=2.501864
PRIMER_LEFT_49_PENALTY=2.235428
PRIMER_RIGHT_49_PENALTY=0.266437
PRIMER_LEFT_49_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_49_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_49=867,20
PRIMER_RIGHT_49=1478,20
PRIMER_LEFT_49_TM=57.765
PRIMER_RIGHT_49_TM=59.734
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=8.8000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=612
=
SEQUENCE_ID=MAGIC.446_2_6262850_6262935_wu-0_sf-2_17200_23974_TRUE
SEQUENCE_TARGET=950,185
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTCTTTNGTCTCCTTCCAAATTCCAATTCATCCCCTTCGTCTTCTTTNTCGGAATCTTANTCGCCAATCTTTCCAAGTTCACATTTTGTTATCTATTTAACTCAAATTTGTAATANTTTTTTNAAATTCACCTCCATCATCCTACTTCGCNTANATGTAATTTCAGTGNTTGTGTNNCTTAGTGNTCTCGATTCATNTAGCTCTTCTCTGCTATNGAATGCNNACAGTCGACGATCANNNNNNNNNNNNTAATTAACNGATTTGGTGGGAGTTCTGTTGATGGNTGCNNNNNNNNNNTTTNCTTTTTTTGTGGGTGNNATCAAAGCCAANTTTGCAGTNTNNNTTTATTCTTTCACNATGNACATGTGTTATATTGTTGTTNACACATCANCACANGAGAGNTTGCCACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_13971386_13971551_ct-1_sf-2_80346_20842_TRUE
SEQUENCE_TARGET=950,265
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17783393_17783743_ct-1_oy-0_6593_10108_TRUE
SEQUENCE_TARGET=950,450
SEQUENCE_TEMPLATE=GTAAAGAGTAAAACCTAGAAAATGTAATATTACCAAATGATGATANTNTAGCAATCAAATCANNAAGANATCTACNTAAAGCCAGTGATCAATTTCTACTTAAAGCATTGTCTTCGTTTTAAGTCAGATTTAACATTANGAATATAAACTTGNCGAANTTANNATGGGATTGGACAAAGCCTATTCATAAAGAAAANTGAAGAGTGTGAGAGTTGATGGGATTGAACATATGGAAAGAGAGGAAGGGAGGTGACAAAACAAAAGAAGAGAAGATGTGTCTTTGTGTGGGGTTTACTAATTTCACAAATCAAGCAAAGCAAACAGTCTTGATTCTCTACACTGTTCTTCCTTTGAATGACTNTACTATTGTTTTGTTTTCATTTCATTTATTACTTAATAATGCAACCCATATATAGTTGAATAATAATTTTNAAAAATACATATTTGGAATTTGGTGGAGACTCAAGTGTCAATCAAAGTCGGTACACTGCCACCATGCTACTGCTTGAACTAATCTTCTCAATGACAACGCCGCTCCNAATATTTTATNNAATATCTTCTATATGATTCACTATATNTCTAACTTATATACTANANTACACATTATGCTAATATCCAAAGGTTCGATGGANAATACTGTTGTANTTATACAAGACTCATTCCAAAAACATATCATANCNTTCTCAGTAGTTGAATTCNAAAAANTAAGGTTTNCANCTAGTCTGCGATCTGCTANAGAGTCTCAAAATCGTAGTTAAAATGAGTGGTTTGTCGTAAATTCGTCTTGATTTTGNTATTTTGAAGATGGAGCANTTTACCATTGTGNATCCTTTACCAAAACTTCTTAGTTACGAATACTTTGGGTTTGTGATAAAATTTTGTGCCCTTCAAATATTTTAATATATGTGTAGTTGTGTATACCAACANATCTCAAAACACTATTATTTCNCTATAAAAANTTTTTGAAATTGGGAAAAATNCATTTTGTTTGGAGACTATTNATTGCACATTGTATCACGATCGAGAAAACCATTAAAAGCAAAGTGACAAAATGAAAAGACTCAAAAAGANGAAACAACAAAGAAGGAAGAAAAATGAGAGTTCCAAAGCAAAAATACNTTGGCCATTCCGATCTCTTTTTGTTTCTGCGTCTCACCGTTTACTTAACTGTTTTCGATCCCTTCCCTGTTGCATCACTTCCTTGNAACAAGTATTAAGACCAAAGAAAATAAAAAAGGTTAAGAGATTTATGCATATTGGAGAGAAATCGTTAAAGCACAGAGTTGACAAGGTACAGTCACGTGGAAGCATTTTGGACATATCGATAATAATCTCTTNCTCTGTTTCAATNATCTTATTCGACGTCAATTGCTCCATACCTCATTCATTGTTCTTGTACGACACAGGCCTTTGTTCATAATNNNNNNNNNNNNNNNNNNNNNTNNATATATACTATCTTATTTCGTAACTCAAAAAGAAATATGATTAATCGTCAACCATATCACCTCGTTACATAAAAGCTAGTGGTTTTGCTTTTTCTAAAAAACCATCGTGTTACTATATCGTATCTAGTATTTTACAAGATCTATAGTTTAAATACTNATATTTTCTGAAANACNAAAAANNNTGAAANTNGTATACTTATATACTATAGAAGAATGCAGAGAATAGTTATTTTCAAAGAGTTGAGGACAAAACTTACGGGGACAAAAAAGTGGATCGAGGAGATTCCAGTGGTCCAATATTGATTTTGGATAGTGGATATGANTATGCGCACGCAATAGCCAATACATCTCAAAAAGTCATTTTTTTGTCAACATCTCAAATGTTTTATGCACCAATATTCAAATGAATTCTCGACTCTACGTTGTTAGTGGATACATCTGCGGTTAATTACAAATGTAACATACACGCATACCATGGAAATATATATATACACATATGGAATTTATGGTATAACCACCACCATGACATTTGTACCATTATACCATAAAAATCATGAAATCGTGTCCAACAATATGATCATTTTAGATTGGACTATCTACAATTATATGTATGAAACGTACGTGGTACTCGATGAGTACGTACATGGTTTAAAGCTTACACGGGATGGGAATTTTGAAAAAGCTATGATGGGCTCTTAAATTCGTTGAAAGCTTACAANAGATGAAACAAAGAAAACCTGCATACATCAAGTACGTACTATAATTACACTAAGGAATGAAAAGAAATCATCAAGGCTTTCATGAAGTATCCGTATCAATAATTTGTTTAGGTTACAGAAATTGACTACAACTAATTTTTATTTTTACGGTTCCATGCAAATTAATCCATTTTAAACAAATCCTCC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.360576
PRIMER_LEFT_0_PENALTY=3.324464
PRIMER_RIGHT_0_PENALTY=0.036112
PRIMER_LEFT_0_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_0_SEQUENCE=CCCCGTAAGTTTTGTCCTCA
PRIMER_LEFT_0=765,22
PRIMER_RIGHT_0=1705,20
PRIMER_LEFT_0_TM=58.676
PRIMER_RIGHT_0_TM=59.964
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=941
PRIMER_PAIR_1_PENALTY=3.372723
PRIMER_LEFT_1_PENALTY=3.324464
PRIMER_RIGHT_1_PENALTY=0.048259
PRIMER_LEFT_1_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_1_SEQUENCE=TGGACCACTGGAATCTCCTC
PRIMER_LEFT_1=765,22
PRIMER_RIGHT_1=1740,20
PRIMER_LEFT_1_TM=58.676
PRIMER_RIGHT_1_TM=60.048
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=976
PRIMER_PAIR_2_PENALTY=3.494513
PRIMER_LEFT_2_PENALTY=3.324464
PRIMER_RIGHT_2_PENALTY=0.170049
PRIMER_LEFT_2_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_2_SEQUENCE=TGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_2=765,22
PRIMER_RIGHT_2=1708,20
PRIMER_LEFT_2_TM=58.676
PRIMER_RIGHT_2_TM=59.830
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=944
PRIMER_PAIR_3_PENALTY=3.712871
PRIMER_LEFT_3_PENALTY=3.324464
PRIMER_RIGHT_3_PENALTY=0.388407
PRIMER_LEFT_3_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_3_SEQUENCE=TGGAATCTCCTCGATCCACT
PRIMER_LEFT_3=765,22
PRIMER_RIGHT_3=1732,20
PRIMER_LEFT_3_TM=58.676
PRIMER_RIGHT_3_TM=59.612
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=968
PRIMER_PAIR_4_PENALTY=3.859612
PRIMER_LEFT_4_PENALTY=3.823500
PRIMER_RIGHT_4_PENALTY=0.036112
PRIMER_LEFT_4_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_4_SEQUENCE=CCCCGTAAGTTTTGTCCTCA
PRIMER_LEFT_4=851,20
PRIMER_RIGHT_4=1705,20
PRIMER_LEFT_4_TM=56.177
PRIMER_RIGHT_4_TM=59.964
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=855
PRIMER_PAIR_5_PENALTY=3.861199
PRIMER_LEFT_5_PENALTY=3.825088
PRIMER_RIGHT_5_PENALTY=0.036112
PRIMER_LEFT_5_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_5_SEQUENCE=CCCCGTAAGTTTTGTCCTCA
PRIMER_LEFT_5=763,22
PRIMER_RIGHT_5=1705,20
PRIMER_LEFT_5_TM=58.175
PRIMER_RIGHT_5_TM=59.964
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=943
PRIMER_PAIR_6_PENALTY=3.861199
PRIMER_LEFT_6_PENALTY=3.825088
PRIMER_RIGHT_6_PENALTY=0.036112
PRIMER_LEFT_6_SEQUENCE=GAGTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_6_SEQUENCE=CCCCGTAAGTTTTGTCCTCA
PRIMER_LEFT_6=761,22
PRIMER_RIGHT_6=1705,20
PRIMER_LEFT_6_TM=58.175
PRIMER_RIGHT_6_TM=59.964
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=945
PRIMER_PAIR_7_PENALTY=3.871759
PRIMER_LEFT_7_PENALTY=3.823500
PRIMER_RIGHT_7_PENALTY=0.048259
PRIMER_LEFT_7_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_7_SEQUENCE=TGGACCACTGGAATCTCCTC
PRIMER_LEFT_7=851,20
PRIMER_RIGHT_7=1740,20
PRIMER_LEFT_7_TM=56.177
PRIMER_RIGHT_7_TM=60.048
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=890
PRIMER_PAIR_8_PENALTY=3.873346
PRIMER_LEFT_8_PENALTY=3.825088
PRIMER_RIGHT_8_PENALTY=0.048259
PRIMER_LEFT_8_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_8_SEQUENCE=TGGACCACTGGAATCTCCTC
PRIMER_LEFT_8=763,22
PRIMER_RIGHT_8=1740,20
PRIMER_LEFT_8_TM=58.175
PRIMER_RIGHT_8_TM=60.048
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=978
PRIMER_PAIR_9_PENALTY=3.873346
PRIMER_LEFT_9_PENALTY=3.825088
PRIMER_RIGHT_9_PENALTY=0.048259
PRIMER_LEFT_9_SEQUENCE=GAGTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_9_SEQUENCE=TGGACCACTGGAATCTCCTC
PRIMER_LEFT_9=761,22
PRIMER_RIGHT_9=1740,20
PRIMER_LEFT_9_TM=58.175
PRIMER_RIGHT_9_TM=60.048
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=980
PRIMER_PAIR_10_PENALTY=3.993549
PRIMER_LEFT_10_PENALTY=3.823500
PRIMER_RIGHT_10_PENALTY=0.170049
PRIMER_LEFT_10_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_10_SEQUENCE=TGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_10=851,20
PRIMER_RIGHT_10=1708,20
PRIMER_LEFT_10_TM=56.177
PRIMER_RIGHT_10_TM=59.830
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=858
PRIMER_PAIR_11_PENALTY=3.995137
PRIMER_LEFT_11_PENALTY=3.825088
PRIMER_RIGHT_11_PENALTY=0.170049
PRIMER_LEFT_11_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_11_SEQUENCE=TGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_11=763,22
PRIMER_RIGHT_11=1708,20
PRIMER_LEFT_11_TM=58.175
PRIMER_RIGHT_11_TM=59.830
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=946
PRIMER_PAIR_12_PENALTY=3.995137
PRIMER_LEFT_12_PENALTY=3.825088
PRIMER_RIGHT_12_PENALTY=0.170049
PRIMER_LEFT_12_SEQUENCE=GAGTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_12_SEQUENCE=TGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_12=761,22
PRIMER_RIGHT_12=1708,20
PRIMER_LEFT_12_TM=58.175
PRIMER_RIGHT_12_TM=59.830
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=948
PRIMER_PAIR_13_PENALTY=4.211907
PRIMER_LEFT_13_PENALTY=3.823500
PRIMER_RIGHT_13_PENALTY=0.388407
PRIMER_LEFT_13_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_13_SEQUENCE=TGGAATCTCCTCGATCCACT
PRIMER_LEFT_13=851,20
PRIMER_RIGHT_13=1732,20
PRIMER_LEFT_13_TM=56.177
PRIMER_RIGHT_13_TM=59.612
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=882
PRIMER_PAIR_14_PENALTY=4.213494
PRIMER_LEFT_14_PENALTY=3.825088
PRIMER_RIGHT_14_PENALTY=0.388407
PRIMER_LEFT_14_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_14_SEQUENCE=TGGAATCTCCTCGATCCACT
PRIMER_LEFT_14=763,22
PRIMER_RIGHT_14=1732,20
PRIMER_LEFT_14_TM=58.175
PRIMER_RIGHT_14_TM=59.612
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=970
PRIMER_PAIR_15_PENALTY=4.296779
PRIMER_LEFT_15_PENALTY=3.324464
PRIMER_RIGHT_15_PENALTY=0.972315
PRIMER_LEFT_15_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_15_SEQUENCE=TCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_15=765,22
PRIMER_RIGHT_15=1706,20
PRIMER_LEFT_15_TM=58.676
PRIMER_RIGHT_15_TM=59.028
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=942
PRIMER_PAIR_16_PENALTY=4.468215
PRIMER_LEFT_16_PENALTY=3.324464
PRIMER_RIGHT_16_PENALTY=1.143751
PRIMER_LEFT_16_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_16_SEQUENCE=GTCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_16=765,22
PRIMER_RIGHT_16=1707,21
PRIMER_LEFT_16_TM=58.676
PRIMER_RIGHT_16_TM=59.856
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=943
PRIMER_PAIR_17_PENALTY=4.795815
PRIMER_LEFT_17_PENALTY=3.823500
PRIMER_RIGHT_17_PENALTY=0.972315
PRIMER_LEFT_17_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_17_SEQUENCE=TCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_17=851,20
PRIMER_RIGHT_17=1706,20
PRIMER_LEFT_17_TM=56.177
PRIMER_RIGHT_17_TM=59.028
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=856
PRIMER_PAIR_18_PENALTY=4.797402
PRIMER_LEFT_18_PENALTY=3.825088
PRIMER_RIGHT_18_PENALTY=0.972315
PRIMER_LEFT_18_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_18_SEQUENCE=TCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_18=763,22
PRIMER_RIGHT_18=1706,20
PRIMER_LEFT_18_TM=58.175
PRIMER_RIGHT_18_TM=59.028
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=944
PRIMER_PAIR_19_PENALTY=4.797402
PRIMER_LEFT_19_PENALTY=3.825088
PRIMER_RIGHT_19_PENALTY=0.972315
PRIMER_LEFT_19_SEQUENCE=GAGTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_19_SEQUENCE=TCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_19=761,22
PRIMER_RIGHT_19=1706,20
PRIMER_LEFT_19_TM=58.175
PRIMER_RIGHT_19_TM=59.028
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=946
PRIMER_PAIR_20_PENALTY=4.797678
PRIMER_LEFT_20_PENALTY=3.324464
PRIMER_RIGHT_20_PENALTY=1.473214
PRIMER_LEFT_20_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_20_SEQUENCE=GGAATCTCCTCGATCCACTTT
PRIMER_LEFT_20=765,22
PRIMER_RIGHT_20=1731,21
PRIMER_LEFT_20_TM=58.676
PRIMER_RIGHT_20_TM=59.527
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=967
PRIMER_PAIR_21_PENALTY=4.811713
PRIMER_LEFT_21_PENALTY=4.775601
PRIMER_RIGHT_21_PENALTY=0.036112
PRIMER_LEFT_21_SEQUENCE=GAGTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_21_SEQUENCE=CCCCGTAAGTTTTGTCCTCA
PRIMER_LEFT_21=761,24
PRIMER_RIGHT_21=1705,20
PRIMER_LEFT_21_TM=60.776
PRIMER_RIGHT_21_TM=59.964
PRIMER_LEFT_21_GC_PERCENT=41.667
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=945
PRIMER_PAIR_22_PENALTY=4.823860
PRIMER_LEFT_22_PENALTY=4.775601
PRIMER_RIGHT_22_PENALTY=0.048259
PRIMER_LEFT_22_SEQUENCE=GAGTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_22_SEQUENCE=TGGACCACTGGAATCTCCTC
PRIMER_LEFT_22=761,24
PRIMER_RIGHT_22=1740,20
PRIMER_LEFT_22_TM=60.776
PRIMER_RIGHT_22_TM=60.048
PRIMER_LEFT_22_GC_PERCENT=41.667
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=980
PRIMER_PAIR_23_PENALTY=4.865532
PRIMER_LEFT_23_PENALTY=4.829420
PRIMER_RIGHT_23_PENALTY=0.036112
PRIMER_LEFT_23_SEQUENCE=ACGAATACTTTGGGTTTGTG
PRIMER_RIGHT_23_SEQUENCE=CCCCGTAAGTTTTGTCCTCA
PRIMER_LEFT_23=850,20
PRIMER_RIGHT_23=1705,20
PRIMER_LEFT_23_TM=55.171
PRIMER_RIGHT_23_TM=59.964
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=856
PRIMER_PAIR_24_PENALTY=4.877506
PRIMER_LEFT_24_PENALTY=4.841394
PRIMER_RIGHT_24_PENALTY=0.036112
PRIMER_LEFT_24_SEQUENCE=GTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_24_SEQUENCE=CCCCGTAAGTTTTGTCCTCA
PRIMER_LEFT_24=763,20
PRIMER_RIGHT_24=1705,20
PRIMER_LEFT_24_TM=55.159
PRIMER_RIGHT_24_TM=59.964
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=943
PRIMER_PAIR_25_PENALTY=4.877678
PRIMER_LEFT_25_PENALTY=4.829420
PRIMER_RIGHT_25_PENALTY=0.048259
PRIMER_LEFT_25_SEQUENCE=ACGAATACTTTGGGTTTGTG
PRIMER_RIGHT_25_SEQUENCE=TGGACCACTGGAATCTCCTC
PRIMER_LEFT_25=850,20
PRIMER_RIGHT_25=1740,20
PRIMER_LEFT_25_TM=55.171
PRIMER_RIGHT_25_TM=60.048
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=891
PRIMER_PAIR_26_PENALTY=4.889652
PRIMER_LEFT_26_PENALTY=4.841394
PRIMER_RIGHT_26_PENALTY=0.048259
PRIMER_LEFT_26_SEQUENCE=GTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_26_SEQUENCE=TGGACCACTGGAATCTCCTC
PRIMER_LEFT_26=763,20
PRIMER_RIGHT_26=1740,20
PRIMER_LEFT_26_TM=55.159
PRIMER_RIGHT_26_TM=60.048
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=978
PRIMER_PAIR_27_PENALTY=4.926097
PRIMER_LEFT_27_PENALTY=3.324464
PRIMER_RIGHT_27_PENALTY=1.601633
PRIMER_LEFT_27_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_27_SEQUENCE=CTGGAATCTCCTCGATCCACT
PRIMER_LEFT_27=765,22
PRIMER_RIGHT_27=1733,21
PRIMER_LEFT_27_TM=58.676
PRIMER_RIGHT_27_TM=60.602
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=969
PRIMER_PAIR_28_PENALTY=4.945650
PRIMER_LEFT_28_PENALTY=4.775601
PRIMER_RIGHT_28_PENALTY=0.170049
PRIMER_LEFT_28_SEQUENCE=GAGTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_28_SEQUENCE=TGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_28=761,24
PRIMER_RIGHT_28=1708,20
PRIMER_LEFT_28_TM=60.776
PRIMER_RIGHT_28_TM=59.830
PRIMER_LEFT_28_GC_PERCENT=41.667
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.1000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=948
PRIMER_PAIR_29_PENALTY=4.967251
PRIMER_LEFT_29_PENALTY=3.823500
PRIMER_RIGHT_29_PENALTY=1.143751
PRIMER_LEFT_29_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_29_SEQUENCE=GTCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_29=851,20
PRIMER_RIGHT_29=1707,21
PRIMER_LEFT_29_TM=56.177
PRIMER_RIGHT_29_TM=59.856
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=857
PRIMER_PAIR_30_PENALTY=4.968839
PRIMER_LEFT_30_PENALTY=3.825088
PRIMER_RIGHT_30_PENALTY=1.143751
PRIMER_LEFT_30_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_30_SEQUENCE=GTCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_30=763,22
PRIMER_RIGHT_30=1707,21
PRIMER_LEFT_30_TM=58.175
PRIMER_RIGHT_30_TM=59.856
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=945
PRIMER_PAIR_31_PENALTY=4.968839
PRIMER_LEFT_31_PENALTY=3.825088
PRIMER_RIGHT_31_PENALTY=1.143751
PRIMER_LEFT_31_SEQUENCE=GAGTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_31_SEQUENCE=GTCCCCGTAAGTTTTGTCCTC
PRIMER_LEFT_31=761,22
PRIMER_RIGHT_31=1707,21
PRIMER_LEFT_31_TM=58.175
PRIMER_RIGHT_31_TM=59.856
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=947
PRIMER_PAIR_32_PENALTY=4.999469
PRIMER_LEFT_32_PENALTY=4.829420
PRIMER_RIGHT_32_PENALTY=0.170049
PRIMER_LEFT_32_SEQUENCE=ACGAATACTTTGGGTTTGTG
PRIMER_RIGHT_32_SEQUENCE=TGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_32=850,20
PRIMER_RIGHT_32=1708,20
PRIMER_LEFT_32_TM=55.171
PRIMER_RIGHT_32_TM=59.830
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=859
PRIMER_PAIR_33_PENALTY=5.011443
PRIMER_LEFT_33_PENALTY=4.841394
PRIMER_RIGHT_33_PENALTY=0.170049
PRIMER_LEFT_33_SEQUENCE=GTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_33_SEQUENCE=TGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_33=763,20
PRIMER_RIGHT_33=1708,20
PRIMER_LEFT_33_TM=55.159
PRIMER_RIGHT_33_TM=59.830
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.4000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=946
PRIMER_PAIR_34_PENALTY=5.082612
PRIMER_LEFT_34_PENALTY=3.324464
PRIMER_RIGHT_34_PENALTY=1.758148
PRIMER_LEFT_34_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_34_SEQUENCE=TGTCCCCGTAAGTTTTGTCCT
PRIMER_LEFT_34=765,22
PRIMER_RIGHT_34=1708,21
PRIMER_LEFT_34_TM=58.676
PRIMER_RIGHT_34_TM=60.758
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=944
PRIMER_PAIR_35_PENALTY=5.206841
PRIMER_LEFT_35_PENALTY=3.324464
PRIMER_RIGHT_35_PENALTY=1.882377
PRIMER_LEFT_35_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_35_SEQUENCE=GGAATCTCCTCGATCCACTT
PRIMER_LEFT_35=765,22
PRIMER_RIGHT_35=1731,20
PRIMER_LEFT_35_TM=58.676
PRIMER_RIGHT_35_TM=58.118
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=967
PRIMER_PAIR_36_PENALTY=5.217826
PRIMER_LEFT_36_PENALTY=4.829420
PRIMER_RIGHT_36_PENALTY=0.388407
PRIMER_LEFT_36_SEQUENCE=ACGAATACTTTGGGTTTGTG
PRIMER_RIGHT_36_SEQUENCE=TGGAATCTCCTCGATCCACT
PRIMER_LEFT_36=850,20
PRIMER_RIGHT_36=1732,20
PRIMER_LEFT_36_TM=55.171
PRIMER_RIGHT_36_TM=59.612
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=883
PRIMER_PAIR_37_PENALTY=5.229800
PRIMER_LEFT_37_PENALTY=4.841394
PRIMER_RIGHT_37_PENALTY=0.388407
PRIMER_LEFT_37_SEQUENCE=GTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_37_SEQUENCE=TGGAATCTCCTCGATCCACT
PRIMER_LEFT_37=763,20
PRIMER_RIGHT_37=1732,20
PRIMER_LEFT_37_TM=55.159
PRIMER_RIGHT_37_TM=59.612
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.4000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=970
PRIMER_PAIR_38_PENALTY=5.295847
PRIMER_LEFT_38_PENALTY=3.324464
PRIMER_RIGHT_38_PENALTY=1.971383
PRIMER_LEFT_38_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_38_SEQUENCE=GTCCCCGTAAGTTTTGTCCT
PRIMER_LEFT_38=765,22
PRIMER_RIGHT_38=1707,20
PRIMER_LEFT_38_TM=58.676
PRIMER_RIGHT_38_TM=58.029
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=943
PRIMER_PAIR_39_PENALTY=5.296714
PRIMER_LEFT_39_PENALTY=3.823500
PRIMER_RIGHT_39_PENALTY=1.473214
PRIMER_LEFT_39_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_39_SEQUENCE=GGAATCTCCTCGATCCACTTT
PRIMER_LEFT_39=851,20
PRIMER_RIGHT_39=1731,21
PRIMER_LEFT_39_TM=56.177
PRIMER_RIGHT_39_TM=59.527
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=881
PRIMER_PAIR_40_PENALTY=5.298301
PRIMER_LEFT_40_PENALTY=3.825088
PRIMER_RIGHT_40_PENALTY=1.473214
PRIMER_LEFT_40_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_40_SEQUENCE=GGAATCTCCTCGATCCACTTT
PRIMER_LEFT_40=763,22
PRIMER_RIGHT_40=1731,21
PRIMER_LEFT_40_TM=58.175
PRIMER_RIGHT_40_TM=59.527
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=969
PRIMER_PAIR_41_PENALTY=5.298301
PRIMER_LEFT_41_PENALTY=3.825088
PRIMER_RIGHT_41_PENALTY=1.473214
PRIMER_LEFT_41_SEQUENCE=GAGTGGTTTGTCGTAAATTCGT
PRIMER_RIGHT_41_SEQUENCE=GGAATCTCCTCGATCCACTTT
PRIMER_LEFT_41=761,22
PRIMER_RIGHT_41=1731,21
PRIMER_LEFT_41_TM=58.175
PRIMER_RIGHT_41_TM=59.527
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.4000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=971
PRIMER_PAIR_42_PENALTY=5.298950
PRIMER_LEFT_42_PENALTY=3.324464
PRIMER_RIGHT_42_PENALTY=1.974486
PRIMER_LEFT_42_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_42_SEQUENCE=TGGAATCTCCTCGATCCACTT
PRIMER_LEFT_42=765,22
PRIMER_RIGHT_42=1732,21
PRIMER_LEFT_42_TM=58.676
PRIMER_RIGHT_42_TM=60.974
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=968
PRIMER_PAIR_43_PENALTY=5.341085
PRIMER_LEFT_43_PENALTY=3.324464
PRIMER_RIGHT_43_PENALTY=2.016621
PRIMER_LEFT_43_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_43_SEQUENCE=TTTGTCCCCGTAAGTTTTGTC
PRIMER_LEFT_43=765,22
PRIMER_RIGHT_43=1710,21
PRIMER_LEFT_43_TM=58.676
PRIMER_RIGHT_43_TM=58.983
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=946
PRIMER_PAIR_44_PENALTY=5.425133
PRIMER_LEFT_44_PENALTY=3.823500
PRIMER_RIGHT_44_PENALTY=1.601633
PRIMER_LEFT_44_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_44_SEQUENCE=CTGGAATCTCCTCGATCCACT
PRIMER_LEFT_44=851,20
PRIMER_RIGHT_44=1733,21
PRIMER_LEFT_44_TM=56.177
PRIMER_RIGHT_44_TM=60.602
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=883
PRIMER_PAIR_45_PENALTY=5.426721
PRIMER_LEFT_45_PENALTY=3.825088
PRIMER_RIGHT_45_PENALTY=1.601633
PRIMER_LEFT_45_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_45_SEQUENCE=CTGGAATCTCCTCGATCCACT
PRIMER_LEFT_45=763,22
PRIMER_RIGHT_45=1733,21
PRIMER_LEFT_45_TM=58.175
PRIMER_RIGHT_45_TM=60.602
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=971
PRIMER_PAIR_46_PENALTY=5.434962
PRIMER_LEFT_46_PENALTY=3.324464
PRIMER_RIGHT_46_PENALTY=2.110498
PRIMER_LEFT_46_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_46_SEQUENCE=TTGTCCCCGTAAGTTTTGTCC
PRIMER_LEFT_46=765,22
PRIMER_RIGHT_46=1709,21
PRIMER_LEFT_46_TM=58.676
PRIMER_RIGHT_46_TM=61.110
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=945
PRIMER_PAIR_47_PENALTY=5.573696
PRIMER_LEFT_47_PENALTY=3.324464
PRIMER_RIGHT_47_PENALTY=2.249232
PRIMER_LEFT_47_SEQUENCE=GGTTTGTCGTAAATTCGTCTTG
PRIMER_RIGHT_47_SEQUENCE=TTTTGTCCCCGTAAGTTTTGTC
PRIMER_LEFT_47=765,22
PRIMER_RIGHT_47=1711,22
PRIMER_LEFT_47_TM=58.676
PRIMER_RIGHT_47_TM=60.249
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=947
PRIMER_PAIR_48_PENALTY=5.581648
PRIMER_LEFT_48_PENALTY=3.823500
PRIMER_RIGHT_48_PENALTY=1.758148
PRIMER_LEFT_48_SEQUENCE=CGAATACTTTGGGTTTGTGA
PRIMER_RIGHT_48_SEQUENCE=TGTCCCCGTAAGTTTTGTCCT
PRIMER_LEFT_48=851,20
PRIMER_RIGHT_48=1708,21
PRIMER_LEFT_48_TM=56.177
PRIMER_RIGHT_48_TM=60.758
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=858
PRIMER_PAIR_49_PENALTY=5.583236
PRIMER_LEFT_49_PENALTY=3.825088
PRIMER_RIGHT_49_PENALTY=1.758148
PRIMER_LEFT_49_SEQUENCE=GTGGTTTGTCGTAAATTCGTCT
PRIMER_RIGHT_49_SEQUENCE=TGTCCCCGTAAGTTTTGTCCT
PRIMER_LEFT_49=763,22
PRIMER_RIGHT_49=1708,21
PRIMER_LEFT_49_TM=58.175
PRIMER_RIGHT_49_TM=60.758
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=946
=
SEQUENCE_ID=MAGIC.287_5_12537263_12537274_ct-1_ws-0_444122_68901_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=TAAGTCAGCTACTTTANACATGTAGNANTGACTNAACAAACGCAAATAAGCAGCAATGAACCTAACAAAGATCACANTAAACNCCACACCGNAACACAACTAAGTCATCTACTTTANACGAGCAGCAATGACCTATATAATTGGTAACAGTTGTAAATGTACAAACCTGGAGGCAGGATTGGAGACAGGTCGGAACGGGGAGAACTCATTNCNGCTCAGCAAAACCTATANANTGAAATNAAATNANAGACTCNANGAATGGTTTGTTTNGATGCAACAGATNNTTGGGTATAGAAGTTCANATAAAAGNACGAAATTGAAAACAGAGNATNNGCATATNTGTCACTCNAGGACAACTTACGAAATGGTTTNTGGAAGATGGAATCCCCTAAGCAGCAANNAAATNAACAGAAAACGANNATTGTTTGGTNAATTTGAAGAAAAAATGACGAAGCTAATNAAGGCAAAGCTNAGACAGTAAACANGGCANNTGTAGGAGAAATAGANNGATGAAGACGTTTCNGNTTACTCGGNCTTCTCNGGCAGGTNNGAGTGGGCAGCNCAATAATGATANANNGAGTTAAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_8427767_8428346_po-0_kn-0_26577_25968_TRUE
SEQUENCE_TARGET=950,679
SEQUENCE_TEMPLATE=CTCTCGTTAAAAGTGTCAANTAAGACATCATTTACACATTACAACACCTAGAAAATGAAAGCCCTTCTTTCACCTCTCTTGTCTCTACTCTCTGTGACTATATNCTCACAAAAAGAATCAGAAACAAGAAATTACCACTAACCTACTAAGCTCTACTNAAAACTATAGACCAAAAATTATGGGTGCAAAACCCCGGAAACACAAAAGAAGGCCGATCCGAAGAGAGGTTTCTTTTCTTCATATCTTTGAGCTTACAAGCAAGCAACTGTTTCAACGNCTATTCACAGCTTNTAGCATCTCCACGTTTCGGTATCTTGTTAAATGCCTGGAGACCAAGCGAGGAGAGTTATGAGGGAAATCATCCAAACCACTGATTTAACAAAGCTTTATATATGATTCTCTTCTTACCTTCACTGTTTAGATCAACTNAAAGATTNGGACTTTTTGTCTGGCTTTCAGGTGACTGGCTATGCAGTGNAGATCCNGTGCAGCCTAGGTTTTGCGAATATGCANCNTTCATCNGTGGCTGACNATTCAGAAACATCTCCAAGCTAGAGGACGCAACTCCTGGACACTTTTTGGCCTTTGAGAATTTTCTGCACATTANGAAAACNTATCAGTTTATAGCGNTTTTGGAATAAGCNTAAGTATATAAATGCACCTGTCTTTNCGCTTTTCACGATATCTTTGCAAAGANACCTTTCTGTTCGCCTGNCCCTCCATACCTTAAGAACGACCCAAAAAACANAGAACGACGGTTAGATTAATAAACTTCACAGGTCATATAACATGCAGAACAACAAGCAAGNCAAGTTTCCTCNAANTTAACAACATTAGNATNGCNTTGCACAGGTGAAGATGTGTTGNTCAAGTTTCGCTCCGAGAATCATTAAGGTAATATNAATACTGTTCATACCAGAATCACGAGAAGAATTCGCCTTCTCAAGGCCTTTTTGNGGAAGTTCNATCATCTTCTCTGCAATAAGATTTNNNATTTTCANTTTGATTNTTGNCTAATTCAAACTANACTCTGAAGAANGAAAGTGGAATCACTATCACAAAAACATNCAAGAGGCATNTNGATATTTTANNCAAGGTAGTATAGCACAATGCAACATTTAGACATACTTCCTTGGTCAACTACCCATGCCTCCTTTCAAAGTTGGACAAAACAAAAGCAGTGTTTGCAAGAAGAGAAAATAAATAAAAAAGCTCTACTGCGTCAGAAAAGCTGGTCCATGAACTTTTTCCAGTTTGATGATACACAAAACAAAATCATTTCANGTTTGAATCATCTATCTATCAACTGAAAAACAGATCAGACACTTTAGCAAAAGCTTACCTTTACTTATGAGCTTTGAAATCATTCTACTGGAAGCAAAAATACCNTTTTCAGGCAAATCAATTGGATTGGCTGCAAAGTGCATGATTGACCGGGCCTGCAAACCAATACANAGGATGAATGATCAAAAACATGCNAACAAAATTTCTATTTTCAGACTGGACAGNATAATGTTATTGATTTACCTTTTCAGGTNGTANTCCATCATANACATTCACTTTTCCACTATAGAATATCNTCATTTGCCCAACGAGCTCACTTGTTTCAGCTGGGCTTCTGNTGCAAGATTACAGACAAGAATGTTTAATANGCTTGTAGAACAACAGGCANNTGCAGCAAAACCTTTTCTTAAGCTNTCGTAGATGGACTAGGGAAAAAACCCTCTAACTTACAAAATATCATTAAGGAATTCAAGTAACTAATTATTATTTCAAGTCACATCAGTCATATTGACTATTATTTTCCATTTCTAAAGAGATCCAGGATACTAAGACATAACGAAGATGATCTTCGATCACACGATCGTGGCCAACCTAAATACTATCTGACAAATCCACCATCAATGCCTTGAACCATATATAGATTCTNTCTCTAAGGATGTGATATTGAACCAAAAACTTCCTAATATCACAAACTTAATAATGCAATGNATTACACTTTTCTTGAATTAAACAACATTCCAGTTCCNGATGCNTAGAAGCAGAAAGGTATNAAAAAAAATTCAAATACGAAAATGTAAACCTGGGAGAAATAGTCGTCTTGTGGCCATCTTTCTCGGNTACAAAGTGACCAGACCCACCGGAAAACTCAGCTGATCTTGGAGAATCCATTCTATGGCACGCGCCGTTATCTTCCGGATAAGAAACCCGGCCACAAGCTTCCAGCTCGTTGCTTGGCTCAATNAACGTTGTTGTGACCTGTAGATATCAGAACAAACAAGGCAACTTAGATTTTCACTTTTTTCAATCTGTGAGTTTAAACGAGAAATAGAAGCAAAATCGAAGTAAATATCTCACGCNAGGCGGATTTACTGGCTGAGAAACGAGGATCTTGCGGAAGATACCGGCGCCGGAATCGTCTCCAGGNTCATAGAGAGCTTTAAGAGATAAAACTTGCTGGATCGCCTGAGATTTGTTCCACGACGGTCTTCGCATTCCTATTTCGACANNTGTCAGCCTCCATTCTCAGAATAAACAAGCNAAAAAAAGCTCCGANAGAGTAGAGAATTTCAAANTTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.932333
PRIMER_LEFT_0_PENALTY=2.341032
PRIMER_RIGHT_0_PENALTY=0.591301
PRIMER_LEFT_0_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_0_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_0=925,21
PRIMER_RIGHT_0=1908,20
PRIMER_LEFT_0_TM=58.659
PRIMER_RIGHT_0_TM=60.591
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=984
PRIMER_PAIR_1_PENALTY=3.064295
PRIMER_LEFT_1_PENALTY=2.341032
PRIMER_RIGHT_1_PENALTY=0.723262
PRIMER_LEFT_1_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_1_SEQUENCE=AGGCATTGATGGTGGATTTG
PRIMER_LEFT_1=925,21
PRIMER_RIGHT_1=1909,20
PRIMER_LEFT_1_TM=58.659
PRIMER_RIGHT_1_TM=60.723
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=985
PRIMER_PAIR_2_PENALTY=3.064295
PRIMER_LEFT_2_PENALTY=2.341032
PRIMER_RIGHT_2_PENALTY=0.723262
PRIMER_LEFT_2_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_2_SEQUENCE=CAAGGCATTGATGGTGGATT
PRIMER_LEFT_2=925,21
PRIMER_RIGHT_2=1911,20
PRIMER_LEFT_2_TM=58.659
PRIMER_RIGHT_2_TM=60.723
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=987
PRIMER_PAIR_3_PENALTY=3.092495
PRIMER_LEFT_3_PENALTY=2.341032
PRIMER_RIGHT_3_PENALTY=0.751463
PRIMER_LEFT_3_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_3_SEQUENCE=AAGGCATTGATGGTGGATTT
PRIMER_LEFT_3=925,21
PRIMER_RIGHT_3=1910,20
PRIMER_LEFT_3_TM=58.659
PRIMER_RIGHT_3_TM=59.249
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=986
PRIMER_PAIR_4_PENALTY=3.566266
PRIMER_LEFT_4_PENALTY=2.341032
PRIMER_RIGHT_4_PENALTY=1.225233
PRIMER_LEFT_4_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_4_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_4=925,21
PRIMER_RIGHT_4=1907,20
PRIMER_LEFT_4_TM=58.659
PRIMER_RIGHT_4_TM=58.775
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=983
PRIMER_PAIR_5_PENALTY=3.835678
PRIMER_LEFT_5_PENALTY=3.244377
PRIMER_RIGHT_5_PENALTY=0.591301
PRIMER_LEFT_5_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_5_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_5=926,20
PRIMER_RIGHT_5=1908,20
PRIMER_LEFT_5_TM=56.756
PRIMER_RIGHT_5_TM=60.591
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=983
PRIMER_PAIR_6_PENALTY=3.967640
PRIMER_LEFT_6_PENALTY=3.244377
PRIMER_RIGHT_6_PENALTY=0.723262
PRIMER_LEFT_6_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_6_SEQUENCE=AGGCATTGATGGTGGATTTG
PRIMER_LEFT_6=926,20
PRIMER_RIGHT_6=1909,20
PRIMER_LEFT_6_TM=56.756
PRIMER_RIGHT_6_TM=60.723
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=984
PRIMER_PAIR_7_PENALTY=3.967640
PRIMER_LEFT_7_PENALTY=3.244377
PRIMER_RIGHT_7_PENALTY=0.723262
PRIMER_LEFT_7_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_7_SEQUENCE=CAAGGCATTGATGGTGGATT
PRIMER_LEFT_7=926,20
PRIMER_RIGHT_7=1911,20
PRIMER_LEFT_7_TM=56.756
PRIMER_RIGHT_7_TM=60.723
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=986
PRIMER_PAIR_8_PENALTY=3.995840
PRIMER_LEFT_8_PENALTY=3.244377
PRIMER_RIGHT_8_PENALTY=0.751463
PRIMER_LEFT_8_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_8_SEQUENCE=AAGGCATTGATGGTGGATTT
PRIMER_LEFT_8=926,20
PRIMER_RIGHT_8=1910,20
PRIMER_LEFT_8_TM=56.756
PRIMER_RIGHT_8_TM=59.249
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=985
PRIMER_PAIR_9_PENALTY=4.469611
PRIMER_LEFT_9_PENALTY=3.244377
PRIMER_RIGHT_9_PENALTY=1.225233
PRIMER_LEFT_9_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_9_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_9=926,20
PRIMER_RIGHT_9=1907,20
PRIMER_LEFT_9_TM=56.756
PRIMER_RIGHT_9_TM=58.775
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=982
PRIMER_PAIR_10_PENALTY=4.505505
PRIMER_LEFT_10_PENALTY=2.341032
PRIMER_RIGHT_10_PENALTY=2.164472
PRIMER_LEFT_10_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_10_SEQUENCE=CATTGATGGTGGATTTGTCAG
PRIMER_LEFT_10=925,21
PRIMER_RIGHT_10=1906,21
PRIMER_LEFT_10_TM=58.659
PRIMER_RIGHT_10_TM=58.836
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=982
PRIMER_PAIR_11_PENALTY=4.853296
PRIMER_LEFT_11_PENALTY=2.341032
PRIMER_RIGHT_11_PENALTY=2.512264
PRIMER_LEFT_11_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_11_SEQUENCE=AGGCATTGATGGTGGATTTGT
PRIMER_LEFT_11=925,21
PRIMER_RIGHT_11=1909,21
PRIMER_LEFT_11_TM=58.659
PRIMER_RIGHT_11_TM=61.512
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=985
PRIMER_PAIR_12_PENALTY=5.094878
PRIMER_LEFT_12_PENALTY=2.341032
PRIMER_RIGHT_12_PENALTY=2.753845
PRIMER_LEFT_12_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_12_SEQUENCE=AGTATTTAGGTTGGCCACGA
PRIMER_LEFT_12=925,21
PRIMER_RIGHT_12=1883,20
PRIMER_LEFT_12_TM=58.659
PRIMER_RIGHT_12_TM=57.246
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=959
PRIMER_PAIR_13_PENALTY=5.168917
PRIMER_LEFT_13_PENALTY=4.577616
PRIMER_RIGHT_13_PENALTY=0.591301
PRIMER_LEFT_13_SEQUENCE=TCATACCAGAATCACGAGAAGA
PRIMER_RIGHT_13_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_13=910,22
PRIMER_RIGHT_13=1908,20
PRIMER_LEFT_13_TM=57.422
PRIMER_RIGHT_13_TM=60.591
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=999
PRIMER_PAIR_14_PENALTY=5.300878
PRIMER_LEFT_14_PENALTY=4.577616
PRIMER_RIGHT_14_PENALTY=0.723262
PRIMER_LEFT_14_SEQUENCE=TCATACCAGAATCACGAGAAGA
PRIMER_RIGHT_14_SEQUENCE=AGGCATTGATGGTGGATTTG
PRIMER_LEFT_14=910,22
PRIMER_RIGHT_14=1909,20
PRIMER_LEFT_14_TM=57.422
PRIMER_RIGHT_14_TM=60.723
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=1000
PRIMER_PAIR_15_PENALTY=5.325971
PRIMER_LEFT_15_PENALTY=2.341032
PRIMER_RIGHT_15_PENALTY=2.984939
PRIMER_LEFT_15_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_15_SEQUENCE=AAGGCATTGATGGTGGATTTG
PRIMER_LEFT_15=925,21
PRIMER_RIGHT_15=1910,21
PRIMER_LEFT_15_TM=58.659
PRIMER_RIGHT_15_TM=61.985
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=986
PRIMER_PAIR_16_PENALTY=5.325971
PRIMER_LEFT_16_PENALTY=2.341032
PRIMER_RIGHT_16_PENALTY=2.984939
PRIMER_LEFT_16_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_16_SEQUENCE=CAAGGCATTGATGGTGGATTT
PRIMER_LEFT_16=925,21
PRIMER_RIGHT_16=1911,21
PRIMER_LEFT_16_TM=58.659
PRIMER_RIGHT_16_TM=61.985
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=987
PRIMER_PAIR_17_PENALTY=5.408850
PRIMER_LEFT_17_PENALTY=3.244377
PRIMER_RIGHT_17_PENALTY=2.164472
PRIMER_LEFT_17_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_17_SEQUENCE=CATTGATGGTGGATTTGTCAG
PRIMER_LEFT_17=926,20
PRIMER_RIGHT_17=1906,21
PRIMER_LEFT_17_TM=56.756
PRIMER_RIGHT_17_TM=58.836
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=981
PRIMER_PAIR_18_PENALTY=5.567109
PRIMER_LEFT_18_PENALTY=4.341875
PRIMER_RIGHT_18_PENALTY=1.225233
PRIMER_LEFT_18_SEQUENCE=GTTCATACCAGAATCACGAGAAGA
PRIMER_RIGHT_18_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_18=908,24
PRIMER_RIGHT_18=1907,20
PRIMER_LEFT_18_TM=59.658
PRIMER_RIGHT_18_TM=58.775
PRIMER_LEFT_18_GC_PERCENT=41.667
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=1000
PRIMER_PAIR_19_PENALTY=5.602317
PRIMER_LEFT_19_PENALTY=5.011016
PRIMER_RIGHT_19_PENALTY=0.591301
PRIMER_LEFT_19_SEQUENCE=CGAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_19_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_19=924,22
PRIMER_RIGHT_19=1908,20
PRIMER_LEFT_19_TM=63.011
PRIMER_RIGHT_19_TM=60.591
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=985
PRIMER_PAIR_20_PENALTY=5.617134
PRIMER_LEFT_20_PENALTY=5.025833
PRIMER_RIGHT_20_PENALTY=0.591301
PRIMER_LEFT_20_SEQUENCE=CATACCAGAATCACGAGAAGAA
PRIMER_RIGHT_20_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_20=911,22
PRIMER_RIGHT_20=1908,20
PRIMER_LEFT_20_TM=56.974
PRIMER_RIGHT_20_TM=60.591
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=998
PRIMER_PAIR_21_PENALTY=5.617134
PRIMER_LEFT_21_PENALTY=5.025833
PRIMER_RIGHT_21_PENALTY=0.591301
PRIMER_LEFT_21_SEQUENCE=TTCATACCAGAATCACGAGAAG
PRIMER_RIGHT_21_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_21=909,22
PRIMER_RIGHT_21=1908,20
PRIMER_LEFT_21_TM=56.974
PRIMER_RIGHT_21_TM=60.591
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=1000
PRIMER_PAIR_22_PENALTY=5.734279
PRIMER_LEFT_22_PENALTY=5.011016
PRIMER_RIGHT_22_PENALTY=0.723262
PRIMER_LEFT_22_SEQUENCE=CGAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_22_SEQUENCE=AGGCATTGATGGTGGATTTG
PRIMER_LEFT_22=924,22
PRIMER_RIGHT_22=1909,20
PRIMER_LEFT_22_TM=63.011
PRIMER_RIGHT_22_TM=60.723
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=986
PRIMER_PAIR_23_PENALTY=5.734279
PRIMER_LEFT_23_PENALTY=5.011016
PRIMER_RIGHT_23_PENALTY=0.723262
PRIMER_LEFT_23_SEQUENCE=CGAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_23_SEQUENCE=CAAGGCATTGATGGTGGATT
PRIMER_LEFT_23=924,22
PRIMER_RIGHT_23=1911,20
PRIMER_LEFT_23_TM=63.011
PRIMER_RIGHT_23_TM=60.723
PRIMER_LEFT_23_GC_PERCENT=45.455
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.1000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=5.749095
PRIMER_LEFT_24_PENALTY=5.025833
PRIMER_RIGHT_24_PENALTY=0.723262
PRIMER_LEFT_24_SEQUENCE=CATACCAGAATCACGAGAAGAA
PRIMER_RIGHT_24_SEQUENCE=AGGCATTGATGGTGGATTTG
PRIMER_LEFT_24=911,22
PRIMER_RIGHT_24=1909,20
PRIMER_LEFT_24_TM=56.974
PRIMER_RIGHT_24_TM=60.723
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=7.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=999
PRIMER_PAIR_25_PENALTY=5.756641
PRIMER_LEFT_25_PENALTY=3.244377
PRIMER_RIGHT_25_PENALTY=2.512264
PRIMER_LEFT_25_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_25_SEQUENCE=AGGCATTGATGGTGGATTTGT
PRIMER_LEFT_25=926,20
PRIMER_RIGHT_25=1909,21
PRIMER_LEFT_25_TM=56.756
PRIMER_RIGHT_25_TM=61.512
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=984
PRIMER_PAIR_26_PENALTY=5.762479
PRIMER_LEFT_26_PENALTY=5.011016
PRIMER_RIGHT_26_PENALTY=0.751463
PRIMER_LEFT_26_SEQUENCE=CGAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_26_SEQUENCE=AAGGCATTGATGGTGGATTT
PRIMER_LEFT_26=924,22
PRIMER_RIGHT_26=1910,20
PRIMER_LEFT_26_TM=63.011
PRIMER_RIGHT_26_TM=59.249
PRIMER_LEFT_26_GC_PERCENT=45.455
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=987
PRIMER_PAIR_27_PENALTY=5.770721
PRIMER_LEFT_27_PENALTY=2.341032
PRIMER_RIGHT_27_PENALTY=3.429689
PRIMER_LEFT_27_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_27_SEQUENCE=GGCATTGATGGTGGATTTGTC
PRIMER_LEFT_27=925,21
PRIMER_RIGHT_27=1908,21
PRIMER_LEFT_27_TM=58.659
PRIMER_RIGHT_27_TM=62.430
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=984
PRIMER_PAIR_28_PENALTY=5.777296
PRIMER_LEFT_28_PENALTY=5.025833
PRIMER_RIGHT_28_PENALTY=0.751463
PRIMER_LEFT_28_SEQUENCE=CATACCAGAATCACGAGAAGAA
PRIMER_RIGHT_28_SEQUENCE=AAGGCATTGATGGTGGATTT
PRIMER_LEFT_28=911,22
PRIMER_RIGHT_28=1910,20
PRIMER_LEFT_28_TM=56.974
PRIMER_RIGHT_28_TM=59.249
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=1000
PRIMER_PAIR_29_PENALTY=5.802849
PRIMER_LEFT_29_PENALTY=4.577616
PRIMER_RIGHT_29_PENALTY=1.225233
PRIMER_LEFT_29_SEQUENCE=TCATACCAGAATCACGAGAAGA
PRIMER_RIGHT_29_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_29=910,22
PRIMER_RIGHT_29=1907,20
PRIMER_LEFT_29_TM=57.422
PRIMER_RIGHT_29_TM=58.775
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=998
PRIMER_PAIR_30_PENALTY=5.912872
PRIMER_LEFT_30_PENALTY=2.341032
PRIMER_RIGHT_30_PENALTY=3.571840
PRIMER_LEFT_30_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_30_SEQUENCE=GATAGTATTTAGGTTGGCCACGA
PRIMER_LEFT_30=925,21
PRIMER_RIGHT_30=1886,23
PRIMER_LEFT_30_TM=58.659
PRIMER_RIGHT_30_TM=59.428
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=43.478
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=962
PRIMER_PAIR_31_PENALTY=5.983485
PRIMER_LEFT_31_PENALTY=2.341032
PRIMER_RIGHT_31_PENALTY=3.642453
PRIMER_LEFT_31_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_31_SEQUENCE=TAGTATTTAGGTTGGCCACGA
PRIMER_LEFT_31=925,21
PRIMER_RIGHT_31=1884,21
PRIMER_LEFT_31_TM=58.659
PRIMER_RIGHT_31_TM=57.358
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=960
PRIMER_PAIR_32_PENALTY=5.998223
PRIMER_LEFT_32_PENALTY=3.244377
PRIMER_RIGHT_32_PENALTY=2.753845
PRIMER_LEFT_32_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_32_SEQUENCE=AGTATTTAGGTTGGCCACGA
PRIMER_LEFT_32=926,20
PRIMER_RIGHT_32=1883,20
PRIMER_LEFT_32_TM=56.756
PRIMER_RIGHT_32_TM=57.246
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=958
PRIMER_PAIR_33_PENALTY=6.195747
PRIMER_LEFT_33_PENALTY=5.604446
PRIMER_RIGHT_33_PENALTY=0.591301
PRIMER_LEFT_33_SEQUENCE=CATACCAGAATCACGAGAAGA
PRIMER_RIGHT_33_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_33=911,21
PRIMER_RIGHT_33=1908,20
PRIMER_LEFT_33_TM=55.396
PRIMER_RIGHT_33_TM=60.591
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=998
PRIMER_PAIR_34_PENALTY=6.195747
PRIMER_LEFT_34_PENALTY=5.604446
PRIMER_RIGHT_34_PENALTY=0.591301
PRIMER_LEFT_34_SEQUENCE=TCATACCAGAATCACGAGAAG
PRIMER_RIGHT_34_SEQUENCE=GGCATTGATGGTGGATTTGT
PRIMER_LEFT_34=910,21
PRIMER_RIGHT_34=1908,20
PRIMER_LEFT_34_TM=55.396
PRIMER_RIGHT_34_TM=60.591
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=999
PRIMER_PAIR_35_PENALTY=6.229316
PRIMER_LEFT_35_PENALTY=3.244377
PRIMER_RIGHT_35_PENALTY=2.984939
PRIMER_LEFT_35_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_35_SEQUENCE=AAGGCATTGATGGTGGATTTG
PRIMER_LEFT_35=926,20
PRIMER_RIGHT_35=1910,21
PRIMER_LEFT_35_TM=56.756
PRIMER_RIGHT_35_TM=61.985
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=985
PRIMER_PAIR_36_PENALTY=6.229316
PRIMER_LEFT_36_PENALTY=3.244377
PRIMER_RIGHT_36_PENALTY=2.984939
PRIMER_LEFT_36_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_36_SEQUENCE=CAAGGCATTGATGGTGGATTT
PRIMER_LEFT_36=926,20
PRIMER_RIGHT_36=1911,21
PRIMER_LEFT_36_TM=56.756
PRIMER_RIGHT_36_TM=61.985
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=986
PRIMER_PAIR_37_PENALTY=6.236250
PRIMER_LEFT_37_PENALTY=5.011016
PRIMER_RIGHT_37_PENALTY=1.225233
PRIMER_LEFT_37_SEQUENCE=CGAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_37_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_37=924,22
PRIMER_RIGHT_37=1907,20
PRIMER_LEFT_37_TM=63.011
PRIMER_RIGHT_37_TM=58.775
PRIMER_LEFT_37_GC_PERCENT=45.455
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=984
PRIMER_PAIR_38_PENALTY=6.251066
PRIMER_LEFT_38_PENALTY=5.025833
PRIMER_RIGHT_38_PENALTY=1.225233
PRIMER_LEFT_38_SEQUENCE=CATACCAGAATCACGAGAAGAA
PRIMER_RIGHT_38_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_38=911,22
PRIMER_RIGHT_38=1907,20
PRIMER_LEFT_38_TM=56.974
PRIMER_RIGHT_38_TM=58.775
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=997
PRIMER_PAIR_39_PENALTY=6.251066
PRIMER_LEFT_39_PENALTY=5.025833
PRIMER_RIGHT_39_PENALTY=1.225233
PRIMER_LEFT_39_SEQUENCE=TTCATACCAGAATCACGAGAAG
PRIMER_RIGHT_39_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_39=909,22
PRIMER_RIGHT_39=1907,20
PRIMER_LEFT_39_TM=56.974
PRIMER_RIGHT_39_TM=58.775
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=999
PRIMER_PAIR_40_PENALTY=6.327708
PRIMER_LEFT_40_PENALTY=5.604446
PRIMER_RIGHT_40_PENALTY=0.723262
PRIMER_LEFT_40_SEQUENCE=CATACCAGAATCACGAGAAGA
PRIMER_RIGHT_40_SEQUENCE=AGGCATTGATGGTGGATTTG
PRIMER_LEFT_40=911,21
PRIMER_RIGHT_40=1909,20
PRIMER_LEFT_40_TM=55.396
PRIMER_RIGHT_40_TM=60.723
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=7.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=999
PRIMER_PAIR_41_PENALTY=6.327708
PRIMER_LEFT_41_PENALTY=5.604446
PRIMER_RIGHT_41_PENALTY=0.723262
PRIMER_LEFT_41_SEQUENCE=TCATACCAGAATCACGAGAAG
PRIMER_RIGHT_41_SEQUENCE=AGGCATTGATGGTGGATTTG
PRIMER_LEFT_41=910,21
PRIMER_RIGHT_41=1909,20
PRIMER_LEFT_41_TM=55.396
PRIMER_RIGHT_41_TM=60.723
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.2000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=1000
PRIMER_PAIR_42_PENALTY=6.345572
PRIMER_LEFT_42_PENALTY=5.120339
PRIMER_RIGHT_42_PENALTY=1.225233
PRIMER_LEFT_42_SEQUENCE=GTTCATACCAGAATCACGAGAAG
PRIMER_RIGHT_42_SEQUENCE=GCATTGATGGTGGATTTGTC
PRIMER_LEFT_42=908,23
PRIMER_RIGHT_42=1907,20
PRIMER_LEFT_42_TM=57.880
PRIMER_RIGHT_42_TM=58.775
PRIMER_LEFT_42_GC_PERCENT=43.478
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=1000
PRIMER_PAIR_43_PENALTY=6.355909
PRIMER_LEFT_43_PENALTY=5.604446
PRIMER_RIGHT_43_PENALTY=0.751463
PRIMER_LEFT_43_SEQUENCE=CATACCAGAATCACGAGAAGA
PRIMER_RIGHT_43_SEQUENCE=AAGGCATTGATGGTGGATTT
PRIMER_LEFT_43=911,21
PRIMER_RIGHT_43=1910,20
PRIMER_LEFT_43_TM=55.396
PRIMER_RIGHT_43_TM=59.249
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=1000
PRIMER_PAIR_44_PENALTY=6.506348
PRIMER_LEFT_44_PENALTY=4.341875
PRIMER_RIGHT_44_PENALTY=2.164472
PRIMER_LEFT_44_SEQUENCE=GTTCATACCAGAATCACGAGAAGA
PRIMER_RIGHT_44_SEQUENCE=CATTGATGGTGGATTTGTCAG
PRIMER_LEFT_44=908,24
PRIMER_RIGHT_44=1906,21
PRIMER_LEFT_44_TM=59.658
PRIMER_RIGHT_44_TM=58.836
PRIMER_LEFT_44_GC_PERCENT=41.667
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=999
PRIMER_PAIR_45_PENALTY=6.569841
PRIMER_LEFT_45_PENALTY=2.341032
PRIMER_RIGHT_45_PENALTY=4.228809
PRIMER_LEFT_45_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_45_SEQUENCE=ATTGATGGTGGATTTGTCAG
PRIMER_LEFT_45=925,21
PRIMER_RIGHT_45=1905,20
PRIMER_LEFT_45_TM=58.659
PRIMER_RIGHT_45_TM=55.771
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=981
PRIMER_PAIR_46_PENALTY=6.602315
PRIMER_LEFT_46_PENALTY=2.341032
PRIMER_RIGHT_46_PENALTY=4.261283
PRIMER_LEFT_46_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_46_SEQUENCE=ATAGTATTTAGGTTGGCCACGA
PRIMER_LEFT_46=925,21
PRIMER_RIGHT_46=1885,22
PRIMER_LEFT_46_TM=58.659
PRIMER_RIGHT_46_TM=57.739
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=961
PRIMER_PAIR_47_PENALTY=6.604015
PRIMER_LEFT_47_PENALTY=2.341032
PRIMER_RIGHT_47_PENALTY=4.262982
PRIMER_LEFT_47_SEQUENCE=GAGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_47_SEQUENCE=AGATAGTATTTAGGTTGGCCACGA
PRIMER_LEFT_47=925,21
PRIMER_RIGHT_47=1887,24
PRIMER_LEFT_47_TM=58.659
PRIMER_RIGHT_47_TM=60.263
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=41.667
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=963
PRIMER_PAIR_48_PENALTY=6.674066
PRIMER_LEFT_48_PENALTY=3.244377
PRIMER_RIGHT_48_PENALTY=3.429689
PRIMER_LEFT_48_SEQUENCE=AGAAGAATTCGCCTTCTCAA
PRIMER_RIGHT_48_SEQUENCE=GGCATTGATGGTGGATTTGTC
PRIMER_LEFT_48=926,20
PRIMER_RIGHT_48=1908,21
PRIMER_LEFT_48_TM=56.756
PRIMER_RIGHT_48_TM=62.430
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=983
PRIMER_PAIR_49_PENALTY=6.697845
PRIMER_LEFT_49_PENALTY=4.533373
PRIMER_RIGHT_49_PENALTY=2.164472
PRIMER_LEFT_49_SEQUENCE=TGTTCATACCAGAATCACGAGAAG
PRIMER_RIGHT_49_SEQUENCE=CATTGATGGTGGATTTGTCAG
PRIMER_LEFT_49=907,24
PRIMER_RIGHT_49=1906,21
PRIMER_LEFT_49_TM=60.533
PRIMER_RIGHT_49_TM=58.836
PRIMER_LEFT_49_GC_PERCENT=41.667
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=1000
=
SEQUENCE_ID=MAGIC.105_1_15729998_15730058_tsu-0_can-0_26976_23221_TRUE
SEQUENCE_TARGET=950,160
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCNATCGCGTTCACATGTGTAATTGACAATTTACAAATGACTNGATTAAGATACTATTTNATCAATTTATCTAGATAAGTTTCATGTCATTTNCTTNGACCACCCTTAANGGTAGTCTATTAACAGAAACTCTAAAAAANANATATTANAGAATAATCAAAAGCTGTNAAAAAAAAGGGAGAGAGAGTGAACAAAAATCCTTATCTAAGANTATTTATCACCANAAAAAAACANGTGTAANGTTANTAGTAATTTGTGTGTATTAACAAAATAAAATATAATCATAACATTAATATATTATTACGTTTTGGTTTTTAAAAAACTCTTACATTTTCATCNGTTGAAGATGCTCTTAAATATCTTTGTTATTTTTACTTGGGTTGTGTNNGNTTGAGACGCGAGAGTATTAATNTCTCTTTTTNTTTTTTNNNNNNNNNNNNNNANCTAGANAGACTANAGCCNNCATNATAGGAATAGGANAGATCCCANAANATACNGATTTNTATTACATNNAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANCCGGAGAAAGNAAAATAAATCAAGAGATTGNTATGNNTTTAGAGAGNTTTATTNTANGATCTNNGANNAAAAANCCATCAAAANANAGAGAATAGATAAGAGATNTAGATATNTANTCAAATACCAAACCTGCATTCCTCNANAAGTAGCTTATATGTTACTTNAACGAAACATCCATCAAGNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_1_15058297_15058579_oy-0_zu-0_1456498_23083_TRUE
SEQUENCE_TARGET=950,382
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_878329_878819_oy-0_mt-0_2648_734106_TRUE
SEQUENCE_TARGET=950,590
SEQUENCE_TEMPLATE=NAGAGAACTNGNATCATTCANGGTTACAAAATTAGCTTCAGACACGAANANACACAACACAAAACNAGANACTAATGATTTNTATTAGNAAATTAACGGAGCAATCAAACTTGAAAGACNGATGATGAAATATNACTTGTTAAAATGTANATCCATGAANAATCGTAATTTATAGTAATCTGNNNNNNTATTGTTGATCAACNAAAANTGTAATTGAGCACTAAANATTTTTTCTCNCTTCTCAGACACNCNTAACAAAAGAAANNTCTCATATCNAACCACTACTAAGCAATCATNACAAATCAAGTAAGATAATCTTCCNAGGACAATCAATAACCATNTCTTTTCGAGTTCATCANNANTTTNAATGTTAGTANTAGNTNTTTTTTGAATTAATACATANACCACNCAAACAAACAAGGTATTCAAGTTAGCACNNNNNNNNNNNNNNNNNNNNNNNAGGTAAGAAAANACCNTGTAACAACCAACANATTCAAGCAANCAAATACTACACAACAAACAACCGNAAAAGTCANAANTTNAAAGCATAAACCACACAAGCACATATTCAAGTTAGCATATTAGACCANACAATACACANATTTAAGTAAGTACAGATCATGNNANNACTAANAAAATTCNANCCATCGCTNCAAAGAATAATCCANAGAANNACCAACNGATTNAAGCCATAGCTTCAAANNATAAACCATNGTCCACATAATAGTCCATAAANCAACNAATAAAATNATATTCTCACGTCTAAATGTTCATAACAATACTAAACAAGTAAAAGGTATCTTTTTCTAACGATTATCTAAATTGTTTACTTACCTAAGACCGNGTTGCANTTAATGTAANAAANTCTTCATTNTTATCAGNNNNAGGTTTTGATTNTGANANTGTTTNTATCTTNGTTGACNTNTTTATACCCTGCACTAAGAAGAGTGATTGTCAAATAAATTCCATTGAATCAGTTGCTCAGGTNTTTAAAGNAGNANCTTGTTAACTTAAGATTTCTNACATTAATANNTATATCAACCAATCGNATNCAAACATAGGAGAAGCACATGCCTTATCCTANAGTAATTTCATATTCTTNCATCAAACAATTNCTAAAAATCACCAAGAAAGTACCTGAGGGATGTTATGCAGAGNCTATTTTTCAATTATCGCNGTGATCTGCGCCATNATCTAAGTATGTAACAATATAAAACTAATTAAATACAAGTTTAAGGAGAAACACATACCAATCTTGAAGTCCATAGACACTTNGAAATCTCTGAGTCTTCAAAAATATCTGGAAAAATAAAAAAGTTGCGTCAGTTATCATTTNGTGCACATANCNTNTTTAAATTTGNGATCTCANTGTGTATGGNNNATTTTATNNNNNNNNNNNNNNNNCCAAGTAGTCACTGGTTTGTNTTAACCTTAAGGGGCTTTTTCTGTTCCCTTTTCCCNTCTGAAACTTGAACCTTAAGNAACTNTCANCTGAACCAAGGGCTCTGTCAAAGCCATGGGTTTGAGATTGTCAATTGTATTTAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCGAGATTCAGGACAAATTGTCTCCGATCTTTTAGATCATCACAGAGGGACAAATTATCCCAATTTAGATTCAGACACAAGGGCAACCAGTAATAATACCGTATGTCTAATCTCTTGGGATTGAACTATATAGCAAATTGGAAACCACAGTTCAGAGAATTACCTGAAGAGTATGTAGACTGTTGCGAAAGCATTCTCGAGAAAACTCTCTGTGATCAATGAGAGAAGAATCCAGTCTCTGTCTTCTTATACGAACATGATCTGCAACAATAAGAAGAAAAAAACAATGTTAGGTAAATATACAAGTCTTTGATCGCTTAACTGTACTCAAAATTAAATTCACGATTACAGATATTGAAACCCTANTTCAAAGAATCAAACACAAATNTTCGATNAATTGGAAGTAAAATCGAGTAAATCAAAACAAAAAAGATCGCAAGATCTTAAAAGAAAAGCGATTTTTGAGAATTTGNAACCCTAAAGNGTTAATTCGATGAAGAGGATTATATAAATCTTTGTTTANNTTTACCCAAATTGAAGAGAGAAACGAGAGAAAGAACTTAAAGGCGANTACTAACCGAAGAGAAAACCATNAATCTCCGATTAGTTATACTCAAATCGAGTACAACAAGGGAAAGAACTTNAAAATTTGAAACCCTAAACATCGATTACAGAAATTACTGACTGAAGAGAACGATAAGTCATGATAAATCCAGATGAAACCGAAGATTTGAACGAAAACTTACAGNGCAAAATTCAAAATCGCCGATCAAAGAGTCGCAGAGAAAGAGAATAATGAAAACCTTTTTTTTTGGCATTATAATAGAAATCTCAGGGAATTTGATACGCTTATATANGCCACGTGGCACNTTCCNAACGTTGTCGNCAACGCNAATAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14438409_14438933_no-0_tsu-0_42216_19391_TRUE
SEQUENCE_TARGET=950,624
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4882356_4883073_wil-2_edi-0_93719_392083_TRUE
SEQUENCE_TARGET=950,817
SEQUENCE_TEMPLATE=TAAAGTGTAGTTGTATTTATTGANAAGCAAGTACCCNCGTTTGAGTAGATGGGAGTGAGAGACAGGTTAGAGCTAAAATCCTCATCTGGTTGTTGAGAATCCTTAAGATTCTTGACGCACGTACGTACGACTTTTCCTAAAAAACAATATTAATATGAGTTTATTACGNTACAAAAAACATTATAATTTTATTCAAATACATGTTNGCTGAAAATAGTTTTATTAATTGGATAGCAGATTCCTTTTTGAGNCTTTTATTAATNTAGAAAACAAAACTATTAAAAANGATCAATTGCTTTAAATGCATGAAAACGAANTACACGCGTTTTTGTAGTACGCAAGCAAGATCAGATGTTTTGGTCTTATTTGTGACTTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCACACGACGTTACGAACATTATTCATTGATAGTCACACGACCACACAAACACCTAACCATAGCTGCTAACATGCCATGTCTAGCCAGCTACTTCTACAAACGTTCATTTAGTTAATTAACTGCTATATTTAACATNTATATACAATAAGAGCTCCAAAATAAGGTAGTGATTTATAAACATCACNTGTACATTTGAAATTNTGAAGAGTTTAGCCATGGAATCAAAACCTCGAAAAACCTTAATTATTTACCATACCATGCCACCAAATTTCCGTAGCAATTCTCAATTTTTGTTCTCTCAATCAAAGTTTTCTCATAAGTTTTGTAGCCGATTTAACACATAAATTTNGAAACTTCCTTTAAAAAANAAACAGTACATGTCCAGAGAATGAATTGGCGACGTCACGTTTGGCCTGGTGCATCTAGTACTCAGAATAAGTATCTNAAAAAGGGTTNGAAAGTTTGCTTTTTGTGTTAAAAAGCCAAANAAAAAGTTCAAAAGTTATATGAGACTNTAATAAAAATGCAAAATGCAGTCTCGCAAAAGATTAATCAGCTGTATTTTTTGTTTACGTTTATGATTCAATAATCTTACTTTCAANAAAAAAATTATTTGCATNATTATTACACTTCAACTTCAGCTAAAAGGCTCTTAGGAAATTGATTGTTCCTAAACACAAATTGTATCTCTTATGTTATTGGTTANNGTTCATACTATTACAANCTTNAATATGATGCNTTATCTANTTTTTTATCAACNATATATATATTATATGAATTCTAATGGGATCATGATACAAACATCTTNNATATATATATTTAAACNAGTGATCTTAATTAANATTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_17028352_17028388_ws-0_zu-0_654_5152643_TRUE
SEQUENCE_TARGET=950,136
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_4_4375038_4375070_tsu-0_ler-0_48873_13536_TRUE
SEQUENCE_TARGET=950,132
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAAATATATNCANTTCGGTGAAATCTAAGTGATGAGTTCGCTCTTCAAAAGACTAGATTTCATCCCTCAAGGATTTTTCCAAGTTTCACCTCTTGGAAAAATATATTCATTTCGGTGAAATCTAAGTGATGAGTTTGCTCTTCACAAGACTAGATTTCATCCCTCAAGGATTTTTCCAAGTTTCACCTCTGGGAAAAGTATATTCATTTCGGTGAAATCTAAGTGATGAGTTCGCTCTTCACAAGACTAGATTTCATCCCTCAAAGATTTTTCCGAGTTCCATCTCTTGGAAAAATATANTTATTTCAGTGAAATTTAAGTNTTGAGTTCGCTCTTCACAAAACTAGATTTCATCCCTCAAGGATTTTTCCGAGTTCCATCTCTTGGAAAAATATATTCATTTCGGTGAAATTTAAGTGTTGAGTTCGCTCTTCACAAAATTAGATTTCATCCCTCAAAGATTTTTCCAAGTTCCACTGCTTGGAAAAATATATTCATTTCGGTGAAATCTAAGTGTTGAGTTCGCTCTTCACAAGACTAGATTTCATTCCTCATCGANTTTTCCAAGTTCCACCTCTTNNAAATATATTCATTTCGNTGAAATTAAGTTTTGAGCTCGCTCTTCACAAGACAAGATTTCATCCCTCAAGNNGTTTTANAAGNTCCACCTCTCANAAAAATGNATTCATTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_19073162_19073883_bur-0_hi-0_17365_623568_TRUE
SEQUENCE_TARGET=950,821
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGGTTAGATAGACGAAACGNCGCGTTTTGTCTTTCNNTTTCGTTTGTCAGATGNAACGNTGCGTTTTTNGTTGNGATGGTCGTCGTCAGNTAGAGAGAGACGCGAANTCNTGATCGCTCTCTCAGGTCTTAGCCCNAAGACTACGAGTCGACTCAGCCGAANTGTNAATGCAGTTTTGNTTTCAATNCCTNAGGTAATTTTGAAANTTTCGGTTTCTCACTCTTNTCNTTGTGCTCTCTCNAGAAAATGTTGTGAAGCTTGAAATGAATCCTTATCTGTACAAGTNAANGCAGAAATTGATGATCTTTGAGGAACTTATTAGACTGGTTTTGATTCGAATTTTATCTCTTGTGTATTTGTTGTTGCTATAAGAACTCTCTTNTTGTTAGCAGGATCTTAGTTATGGCTACTTGCTTGCGTNGNATCANGAAGAGAGTTAATCTTCTACAGAGACGAGTTTATCCATCGTGTGGTCATCTAATTCGTGATGATAGAGATGAAACTAAATCTGGTTCTTCGGATACAANGATTCGTGAAGTCTTAGCACGTAATGGGACTNATAAGCTTAGCTCTATGTTTGCGGATAGGCATTATCAGTCGTTTGCTACTGGTCCGTTGGGTCTTGGATTGTCTTCGTGTCGGCATATGAGTTCTACACCACCNGAATGGTCTGANAAAGTTGATGGTATNNACTTTGTGNCTACGGANGTTGTTCCCGATGAAATCATTGAAGCTGTGACNACCACAAGCCAAGCTGTTGTTCCTGCTATAAACGAGGTTGCTATTGCTGCTGCCGATTCTGCTTNTCCTGTTGCTGCTTTGCAGCATTTGATTGATGCTGTGCATTCCTTTACTGGCCTGAACTGGTATNATATNTANACTGTTTTNAAACTCATTNTNTTTGGNTATGNNNTTTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTGGCAGGTGGGCTTCAATAGCCTTGACAACTGTTCTTATCCGTGGAGTTACAATACCTATTCTTTTGAATCAACTAAAGGCTACTTACAAACTCAATGTCAGTTTTTAGATTTTTGTTCTCTGATTGCNTTTTTCAGTCACNAGGATTTGCCTCTTTTCCATTTGTGATTNATCTATCATGTTCTTGCAGGTTCTGAGGCCACAACTGGAGGAGTTAAGACAAGAGATGAGCACTAAGGTACAATCTGTTTCTGCGCCTTCTCTTACTTCNTCTTTGTGTGCANANGAGCGTTTGTGAGTTTGACACTGTTCAAGGCCGTGAAAANGCCTTCTGGATAAATATAAAGATAAANTCAGNTTGTCATAGTTAGTAANTGNATGATTCCTTCAGGGTTTTCTAANTATCACTTGTGAACTATAATCTTGATTGAGTAGCTTTCTCGAGTTTTCTGTTTGGTTAGGAATTGTCGATNAAGTTCCAACTAGCGTTTCTAGANTGTCGTGGTTAAGACCTTTTGCATGTGTNATAATNNTTTTTAGGCTCAGGATCCTGAAGCCATGGCAGAAGGTCAAAGACGGATGCAATTATTGTTTAAANAGTAAGTCCACTTTTATGGATGACTGGTGGAGATGATTTATCTCTTCAANTTAAACNTACTACTAGTTCCTCATCNACTGATATGCTTCTATTGTTTCGTGGANTGGCTTAGACATGGAGTAACTCCATTTACCCCCCTCAAAGGACTTATTATTCAAGGTCCCATCTTCATCAGCTTTTTCTTTGCGGTAAGATGTTTATACGTATATACTCAAGNNNTTTACTGTTTCTGATTNTTATTGTCTATGTGTTCACTGATGTTTGTCTACAGATCAGGAATATGGCNGAGAAAGNCCCATCATTTAAAACCGGGGGAACTCTTTGGTTTANNGATCTNACCACCACAGATACCACTTATATCTTGCCACTCCTCACTGCAGTGACTTTCTTGATTATGGTAGAGGTAAGAGCTTATACTTTCTTGTTGGCCTATTCATGACTAACTTTTGGTAGTTGGTTATATTTCATGACTAATTTATCTAACCCAAGTCTTGTTTCTCTTTTNACATAGTCAAACATGCAGGAAGGTCTTGAAGGCAATCCAGTAGCTGGAACTATGAAGAAATTCTCAAGAATCATTGCCTTCCTTTCTATTCCAGTTTTGATTGGCATTGAAAAGGTAAAGCNTAATGTTCCTCTATTGACTTTTTGATGTTCATAAGCATTGGAAACGTAAGAAAAATGACATTTTGCTTACCACAAACGAATGANTTTTTGTCTGNAGGCATTGTTTTGTTACTGGCTTACTTCCAACCTNTTTACTCTTGTGTATGGATTAAGTAAGTCCCCTGAACATTGTTCTTACNCTTTGAGACGTGAGTTTTGCAAATCCTGAAGAGNNNTTTTNATTTGTATCTTTTNCTTGTGTANATATGCAGCATTAAGACGTCCTGATGTGAGGAAGCTCTTGAATCTCCCAGATGTTGTCAACTCTTCTACTAGGCAGCCATCACCGTCTTCGCCTTTGCCATTTTCCTTTGCCGAGCCAAAAGACCAAAGTGTTGTTGCTCAAGAAAAACCTCCTNTNTCCTCCGAGTCATCTTCAAGTGTTCCAGATAGAAGAATCTCACGGAGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.915510
PRIMER_LEFT_0_PENALTY=0.039813
PRIMER_RIGHT_0_PENALTY=0.875697
PRIMER_LEFT_0_SEQUENCE=GCTTTGCAGCATTTGATTGA
PRIMER_RIGHT_0_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_0=911,20
PRIMER_RIGHT_0=1834,20
PRIMER_LEFT_0_TM=59.960
PRIMER_RIGHT_0_TM=60.876
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=924
PRIMER_PAIR_1_PENALTY=0.920154
PRIMER_LEFT_1_PENALTY=0.044457
PRIMER_RIGHT_1_PENALTY=0.875697
PRIMER_LEFT_1_SEQUENCE=ACGAGGTTGCTATTGCTGCT
PRIMER_RIGHT_1_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_1=867,20
PRIMER_RIGHT_1=1834,20
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=60.876
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=968
PRIMER_PAIR_2_PENALTY=1.128248
PRIMER_LEFT_2_PENALTY=0.039813
PRIMER_RIGHT_2_PENALTY=1.088435
PRIMER_LEFT_2_SEQUENCE=GCTTTGCAGCATTTGATTGA
PRIMER_RIGHT_2_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_2=911,20
PRIMER_RIGHT_2=1823,21
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=60.088
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=913
PRIMER_PAIR_3_PENALTY=1.132892
PRIMER_LEFT_3_PENALTY=0.044457
PRIMER_RIGHT_3_PENALTY=1.088435
PRIMER_LEFT_3_SEQUENCE=ACGAGGTTGCTATTGCTGCT
PRIMER_RIGHT_3_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_3=867,20
PRIMER_RIGHT_3=1823,21
PRIMER_LEFT_3_TM=60.044
PRIMER_RIGHT_3_TM=60.088
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=957
PRIMER_PAIR_4_PENALTY=1.174595
PRIMER_LEFT_4_PENALTY=0.298898
PRIMER_RIGHT_4_PENALTY=0.875697
PRIMER_LEFT_4_SEQUENCE=ACAAGCCAAGCTGTTGTTCC
PRIMER_RIGHT_4_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_4=839,20
PRIMER_RIGHT_4=1834,20
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=60.876
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=996
PRIMER_PAIR_5_PENALTY=1.307183
PRIMER_LEFT_5_PENALTY=0.431486
PRIMER_RIGHT_5_PENALTY=0.875697
PRIMER_LEFT_5_SEQUENCE=AAGCCAAGCTGTTGTTCCTG
PRIMER_RIGHT_5_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_5=841,20
PRIMER_RIGHT_5=1834,20
PRIMER_LEFT_5_TM=60.431
PRIMER_RIGHT_5_TM=60.876
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=994
PRIMER_PAIR_6_PENALTY=1.307183
PRIMER_LEFT_6_PENALTY=0.431486
PRIMER_RIGHT_6_PENALTY=0.875697
PRIMER_LEFT_6_SEQUENCE=CAAGCCAAGCTGTTGTTCCT
PRIMER_RIGHT_6_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_6=840,20
PRIMER_RIGHT_6=1834,20
PRIMER_LEFT_6_TM=60.431
PRIMER_RIGHT_6_TM=60.876
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=995
PRIMER_PAIR_7_PENALTY=1.340535
PRIMER_LEFT_7_PENALTY=0.039813
PRIMER_RIGHT_7_PENALTY=1.300722
PRIMER_LEFT_7_SEQUENCE=GCTTTGCAGCATTTGATTGA
PRIMER_RIGHT_7_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_7=911,20
PRIMER_RIGHT_7=1836,20
PRIMER_LEFT_7_TM=59.960
PRIMER_RIGHT_7_TM=58.699
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=926
PRIMER_PAIR_8_PENALTY=1.345179
PRIMER_LEFT_8_PENALTY=0.044457
PRIMER_RIGHT_8_PENALTY=1.300722
PRIMER_LEFT_8_SEQUENCE=ACGAGGTTGCTATTGCTGCT
PRIMER_RIGHT_8_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_8=867,20
PRIMER_RIGHT_8=1836,20
PRIMER_LEFT_8_TM=60.044
PRIMER_RIGHT_8_TM=58.699
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=970
PRIMER_PAIR_9_PENALTY=1.346850
PRIMER_LEFT_9_PENALTY=0.039813
PRIMER_RIGHT_9_PENALTY=1.307037
PRIMER_LEFT_9_SEQUENCE=GCTTTGCAGCATTTGATTGA
PRIMER_RIGHT_9_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_9=911,20
PRIMER_RIGHT_9=1822,20
PRIMER_LEFT_9_TM=59.960
PRIMER_RIGHT_9_TM=58.693
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=912
PRIMER_PAIR_10_PENALTY=1.351494
PRIMER_LEFT_10_PENALTY=0.044457
PRIMER_RIGHT_10_PENALTY=1.307037
PRIMER_LEFT_10_SEQUENCE=ACGAGGTTGCTATTGCTGCT
PRIMER_RIGHT_10_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_10=867,20
PRIMER_RIGHT_10=1822,20
PRIMER_LEFT_10_TM=60.044
PRIMER_RIGHT_10_TM=58.693
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=956
PRIMER_PAIR_11_PENALTY=1.385621
PRIMER_LEFT_11_PENALTY=0.509923
PRIMER_RIGHT_11_PENALTY=0.875697
PRIMER_LEFT_11_SEQUENCE=CACAAGCCAAGCTGTTGTTC
PRIMER_RIGHT_11_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_11=838,20
PRIMER_RIGHT_11=1834,20
PRIMER_LEFT_11_TM=59.490
PRIMER_RIGHT_11_TM=60.876
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=997
PRIMER_PAIR_12_PENALTY=1.386421
PRIMER_LEFT_12_PENALTY=0.510724
PRIMER_RIGHT_12_PENALTY=0.875697
PRIMER_LEFT_12_SEQUENCE=CTATTGCTGCTGCCGATTCT
PRIMER_RIGHT_12_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_12=876,20
PRIMER_RIGHT_12=1834,20
PRIMER_LEFT_12_TM=60.511
PRIMER_RIGHT_12_TM=60.876
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=959
PRIMER_PAIR_13_PENALTY=1.387333
PRIMER_LEFT_13_PENALTY=0.298898
PRIMER_RIGHT_13_PENALTY=1.088435
PRIMER_LEFT_13_SEQUENCE=ACAAGCCAAGCTGTTGTTCC
PRIMER_RIGHT_13_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_13=839,20
PRIMER_RIGHT_13=1823,21
PRIMER_LEFT_13_TM=60.299
PRIMER_RIGHT_13_TM=60.088
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=985
PRIMER_PAIR_14_PENALTY=1.516596
PRIMER_LEFT_14_PENALTY=0.640899
PRIMER_RIGHT_14_PENALTY=0.875697
PRIMER_LEFT_14_SEQUENCE=TGATTGATGCTGTGCATTCC
PRIMER_RIGHT_14_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_14=924,20
PRIMER_RIGHT_14=1834,20
PRIMER_LEFT_14_TM=60.641
PRIMER_RIGHT_14_TM=60.876
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=911
PRIMER_PAIR_15_PENALTY=1.519921
PRIMER_LEFT_15_PENALTY=0.431486
PRIMER_RIGHT_15_PENALTY=1.088435
PRIMER_LEFT_15_SEQUENCE=AAGCCAAGCTGTTGTTCCTG
PRIMER_RIGHT_15_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_15=841,20
PRIMER_RIGHT_15=1823,21
PRIMER_LEFT_15_TM=60.431
PRIMER_RIGHT_15_TM=60.088
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=983
PRIMER_PAIR_16_PENALTY=1.519921
PRIMER_LEFT_16_PENALTY=0.431486
PRIMER_RIGHT_16_PENALTY=1.088435
PRIMER_LEFT_16_SEQUENCE=CAAGCCAAGCTGTTGTTCCT
PRIMER_RIGHT_16_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_16=840,20
PRIMER_RIGHT_16=1823,21
PRIMER_LEFT_16_TM=60.431
PRIMER_RIGHT_16_TM=60.088
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=984
PRIMER_PAIR_17_PENALTY=1.598359
PRIMER_LEFT_17_PENALTY=0.509923
PRIMER_RIGHT_17_PENALTY=1.088435
PRIMER_LEFT_17_SEQUENCE=CACAAGCCAAGCTGTTGTTC
PRIMER_RIGHT_17_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_17=838,20
PRIMER_RIGHT_17=1823,21
PRIMER_LEFT_17_TM=59.490
PRIMER_RIGHT_17_TM=60.088
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=986
PRIMER_PAIR_18_PENALTY=1.599159
PRIMER_LEFT_18_PENALTY=0.510724
PRIMER_RIGHT_18_PENALTY=1.088435
PRIMER_LEFT_18_SEQUENCE=CTATTGCTGCTGCCGATTCT
PRIMER_RIGHT_18_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_18=876,20
PRIMER_RIGHT_18=1823,21
PRIMER_LEFT_18_TM=60.511
PRIMER_RIGHT_18_TM=60.088
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=948
PRIMER_PAIR_19_PENALTY=1.599315
PRIMER_LEFT_19_PENALTY=0.723618
PRIMER_RIGHT_19_PENALTY=0.875697
PRIMER_LEFT_19_SEQUENCE=TTGATGCTGTGCATTCCTTT
PRIMER_RIGHT_19_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_19=927,20
PRIMER_RIGHT_19=1834,20
PRIMER_LEFT_19_TM=59.276
PRIMER_RIGHT_19_TM=60.876
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=908
PRIMER_PAIR_20_PENALTY=1.599620
PRIMER_LEFT_20_PENALTY=0.298898
PRIMER_RIGHT_20_PENALTY=1.300722
PRIMER_LEFT_20_SEQUENCE=ACAAGCCAAGCTGTTGTTCC
PRIMER_RIGHT_20_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_20=839,20
PRIMER_RIGHT_20=1836,20
PRIMER_LEFT_20_TM=60.299
PRIMER_RIGHT_20_TM=58.699
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=998
PRIMER_PAIR_21_PENALTY=1.605935
PRIMER_LEFT_21_PENALTY=0.298898
PRIMER_RIGHT_21_PENALTY=1.307037
PRIMER_LEFT_21_SEQUENCE=ACAAGCCAAGCTGTTGTTCC
PRIMER_RIGHT_21_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_21=839,20
PRIMER_RIGHT_21=1822,20
PRIMER_LEFT_21_TM=60.299
PRIMER_RIGHT_21_TM=58.693
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=984
PRIMER_PAIR_22_PENALTY=1.729334
PRIMER_LEFT_22_PENALTY=0.640899
PRIMER_RIGHT_22_PENALTY=1.088435
PRIMER_LEFT_22_SEQUENCE=TGATTGATGCTGTGCATTCC
PRIMER_RIGHT_22_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_22=924,20
PRIMER_RIGHT_22=1823,21
PRIMER_LEFT_22_TM=60.641
PRIMER_RIGHT_22_TM=60.088
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=900
PRIMER_PAIR_23_PENALTY=1.732208
PRIMER_LEFT_23_PENALTY=0.431486
PRIMER_RIGHT_23_PENALTY=1.300722
PRIMER_LEFT_23_SEQUENCE=AAGCCAAGCTGTTGTTCCTG
PRIMER_RIGHT_23_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_23=841,20
PRIMER_RIGHT_23=1836,20
PRIMER_LEFT_23_TM=60.431
PRIMER_RIGHT_23_TM=58.699
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=996
PRIMER_PAIR_24_PENALTY=1.732208
PRIMER_LEFT_24_PENALTY=0.431486
PRIMER_RIGHT_24_PENALTY=1.300722
PRIMER_LEFT_24_SEQUENCE=CAAGCCAAGCTGTTGTTCCT
PRIMER_RIGHT_24_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_24=840,20
PRIMER_RIGHT_24=1836,20
PRIMER_LEFT_24_TM=60.431
PRIMER_RIGHT_24_TM=58.699
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=997
PRIMER_PAIR_25_PENALTY=1.738523
PRIMER_LEFT_25_PENALTY=0.431486
PRIMER_RIGHT_25_PENALTY=1.307037
PRIMER_LEFT_25_SEQUENCE=AAGCCAAGCTGTTGTTCCTG
PRIMER_RIGHT_25_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_25=841,20
PRIMER_RIGHT_25=1822,20
PRIMER_LEFT_25_TM=60.431
PRIMER_RIGHT_25_TM=58.693
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=982
PRIMER_PAIR_26_PENALTY=1.738523
PRIMER_LEFT_26_PENALTY=0.431486
PRIMER_RIGHT_26_PENALTY=1.307037
PRIMER_LEFT_26_SEQUENCE=CAAGCCAAGCTGTTGTTCCT
PRIMER_RIGHT_26_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_26=840,20
PRIMER_RIGHT_26=1822,20
PRIMER_LEFT_26_TM=60.431
PRIMER_RIGHT_26_TM=58.693
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=983
PRIMER_PAIR_27_PENALTY=1.810066
PRIMER_LEFT_27_PENALTY=0.934369
PRIMER_RIGHT_27_PENALTY=0.875697
PRIMER_LEFT_27_SEQUENCE=CTGCTTTGCAGCATTTGATT
PRIMER_RIGHT_27_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_27=909,20
PRIMER_RIGHT_27=1834,20
PRIMER_LEFT_27_TM=59.066
PRIMER_RIGHT_27_TM=60.876
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=926
PRIMER_PAIR_28_PENALTY=1.810645
PRIMER_LEFT_28_PENALTY=0.509923
PRIMER_RIGHT_28_PENALTY=1.300722
PRIMER_LEFT_28_SEQUENCE=CACAAGCCAAGCTGTTGTTC
PRIMER_RIGHT_28_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_28=838,20
PRIMER_RIGHT_28=1836,20
PRIMER_LEFT_28_TM=59.490
PRIMER_RIGHT_28_TM=58.699
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=999
PRIMER_PAIR_29_PENALTY=1.811446
PRIMER_LEFT_29_PENALTY=0.510724
PRIMER_RIGHT_29_PENALTY=1.300722
PRIMER_LEFT_29_SEQUENCE=CTATTGCTGCTGCCGATTCT
PRIMER_RIGHT_29_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_29=876,20
PRIMER_RIGHT_29=1836,20
PRIMER_LEFT_29_TM=60.511
PRIMER_RIGHT_29_TM=58.699
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.6000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=961
PRIMER_PAIR_30_PENALTY=1.812053
PRIMER_LEFT_30_PENALTY=0.723618
PRIMER_RIGHT_30_PENALTY=1.088435
PRIMER_LEFT_30_SEQUENCE=TTGATGCTGTGCATTCCTTT
PRIMER_RIGHT_30_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_30=927,20
PRIMER_RIGHT_30=1823,21
PRIMER_LEFT_30_TM=59.276
PRIMER_RIGHT_30_TM=60.088
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=897
PRIMER_PAIR_31_PENALTY=1.816960
PRIMER_LEFT_31_PENALTY=0.509923
PRIMER_RIGHT_31_PENALTY=1.307037
PRIMER_LEFT_31_SEQUENCE=CACAAGCCAAGCTGTTGTTC
PRIMER_RIGHT_31_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_31=838,20
PRIMER_RIGHT_31=1822,20
PRIMER_LEFT_31_TM=59.490
PRIMER_RIGHT_31_TM=58.693
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=985
PRIMER_PAIR_32_PENALTY=1.817761
PRIMER_LEFT_32_PENALTY=0.510724
PRIMER_RIGHT_32_PENALTY=1.307037
PRIMER_LEFT_32_SEQUENCE=CTATTGCTGCTGCCGATTCT
PRIMER_RIGHT_32_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_32=876,20
PRIMER_RIGHT_32=1822,20
PRIMER_LEFT_32_TM=60.511
PRIMER_RIGHT_32_TM=58.693
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=947
PRIMER_PAIR_33_PENALTY=1.941621
PRIMER_LEFT_33_PENALTY=0.640899
PRIMER_RIGHT_33_PENALTY=1.300722
PRIMER_LEFT_33_SEQUENCE=TGATTGATGCTGTGCATTCC
PRIMER_RIGHT_33_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_33=924,20
PRIMER_RIGHT_33=1836,20
PRIMER_LEFT_33_TM=60.641
PRIMER_RIGHT_33_TM=58.699
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=913
PRIMER_PAIR_34_PENALTY=1.947936
PRIMER_LEFT_34_PENALTY=0.640899
PRIMER_RIGHT_34_PENALTY=1.307037
PRIMER_LEFT_34_SEQUENCE=TGATTGATGCTGTGCATTCC
PRIMER_RIGHT_34_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_34=924,20
PRIMER_RIGHT_34=1822,20
PRIMER_LEFT_34_TM=60.641
PRIMER_RIGHT_34_TM=58.693
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=899
PRIMER_PAIR_35_PENALTY=1.968475
PRIMER_LEFT_35_PENALTY=0.039813
PRIMER_RIGHT_35_PENALTY=1.928663
PRIMER_LEFT_35_SEQUENCE=GCTTTGCAGCATTTGATTGA
PRIMER_RIGHT_35_SEQUENCE=CTGATGAAGATGGGACCTTG
PRIMER_LEFT_35=911,20
PRIMER_RIGHT_35=1820,20
PRIMER_LEFT_35_TM=59.960
PRIMER_RIGHT_35_TM=58.071
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=910
PRIMER_PAIR_36_PENALTY=1.971498
PRIMER_LEFT_36_PENALTY=1.095801
PRIMER_RIGHT_36_PENALTY=0.875697
PRIMER_LEFT_36_SEQUENCE=GATTGATGCTGTGCATTCCTT
PRIMER_RIGHT_36_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_36=925,21
PRIMER_RIGHT_36=1834,20
PRIMER_LEFT_36_TM=60.096
PRIMER_RIGHT_36_TM=60.876
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=910
PRIMER_PAIR_37_PENALTY=1.973120
PRIMER_LEFT_37_PENALTY=0.044457
PRIMER_RIGHT_37_PENALTY=1.928663
PRIMER_LEFT_37_SEQUENCE=ACGAGGTTGCTATTGCTGCT
PRIMER_RIGHT_37_SEQUENCE=CTGATGAAGATGGGACCTTG
PRIMER_LEFT_37=867,20
PRIMER_RIGHT_37=1820,20
PRIMER_LEFT_37_TM=60.044
PRIMER_RIGHT_37_TM=58.071
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=954
PRIMER_PAIR_38_PENALTY=2.022804
PRIMER_LEFT_38_PENALTY=0.934369
PRIMER_RIGHT_38_PENALTY=1.088435
PRIMER_LEFT_38_SEQUENCE=CTGCTTTGCAGCATTTGATT
PRIMER_RIGHT_38_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_38=909,20
PRIMER_RIGHT_38=1823,21
PRIMER_LEFT_38_TM=59.066
PRIMER_RIGHT_38_TM=60.088
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=915
PRIMER_PAIR_39_PENALTY=2.024340
PRIMER_LEFT_39_PENALTY=0.723618
PRIMER_RIGHT_39_PENALTY=1.300722
PRIMER_LEFT_39_SEQUENCE=TTGATGCTGTGCATTCCTTT
PRIMER_RIGHT_39_SEQUENCE=TACCGCAAAGAAAAAGCTGA
PRIMER_LEFT_39=927,20
PRIMER_RIGHT_39=1836,20
PRIMER_LEFT_39_TM=59.276
PRIMER_RIGHT_39_TM=58.699
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=910
PRIMER_PAIR_40_PENALTY=2.030655
PRIMER_LEFT_40_PENALTY=0.723618
PRIMER_RIGHT_40_PENALTY=1.307037
PRIMER_LEFT_40_SEQUENCE=TTGATGCTGTGCATTCCTTT
PRIMER_RIGHT_40_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_40=927,20
PRIMER_RIGHT_40=1822,20
PRIMER_LEFT_40_TM=59.276
PRIMER_RIGHT_40_TM=58.693
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=896
PRIMER_PAIR_41_PENALTY=2.148183
PRIMER_LEFT_41_PENALTY=1.272486
PRIMER_RIGHT_41_PENALTY=0.875697
PRIMER_LEFT_41_SEQUENCE=CAGCATTTGATTGATGCTGTG
PRIMER_RIGHT_41_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_41=917,21
PRIMER_RIGHT_41=1834,20
PRIMER_LEFT_41_TM=60.272
PRIMER_RIGHT_41_TM=60.876
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=918
PRIMER_PAIR_42_PENALTY=2.184236
PRIMER_LEFT_42_PENALTY=1.095801
PRIMER_RIGHT_42_PENALTY=1.088435
PRIMER_LEFT_42_SEQUENCE=GATTGATGCTGTGCATTCCTT
PRIMER_RIGHT_42_SEQUENCE=AAGCTGATGAAGATGGGACCT
PRIMER_LEFT_42=925,21
PRIMER_RIGHT_42=1823,21
PRIMER_LEFT_42_TM=60.096
PRIMER_RIGHT_42_TM=60.088
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=899
PRIMER_PAIR_43_PENALTY=2.201790
PRIMER_LEFT_43_PENALTY=1.326093
PRIMER_RIGHT_43_PENALTY=0.875697
PRIMER_LEFT_43_SEQUENCE=GATTGATGCTGTGCATTCCT
PRIMER_RIGHT_43_SEQUENCE=CCGCAAAGAAAAAGCTGATG
PRIMER_LEFT_43=925,20
PRIMER_RIGHT_43=1834,20
PRIMER_LEFT_43_TM=58.674
PRIMER_RIGHT_43_TM=60.876
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=910
PRIMER_PAIR_44_PENALTY=2.227560
PRIMER_LEFT_44_PENALTY=0.298898
PRIMER_RIGHT_44_PENALTY=1.928663
PRIMER_LEFT_44_SEQUENCE=ACAAGCCAAGCTGTTGTTCC
PRIMER_RIGHT_44_SEQUENCE=CTGATGAAGATGGGACCTTG
PRIMER_LEFT_44=839,20
PRIMER_RIGHT_44=1820,20
PRIMER_LEFT_44_TM=60.299
PRIMER_RIGHT_44_TM=58.071
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=982
PRIMER_PAIR_45_PENALTY=2.241406
PRIMER_LEFT_45_PENALTY=0.934369
PRIMER_RIGHT_45_PENALTY=1.307037
PRIMER_LEFT_45_SEQUENCE=CTGCTTTGCAGCATTTGATT
PRIMER_RIGHT_45_SEQUENCE=AGCTGATGAAGATGGGACCT
PRIMER_LEFT_45=909,20
PRIMER_RIGHT_45=1822,20
PRIMER_LEFT_45_TM=59.066
PRIMER_RIGHT_45_TM=58.693
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=914
PRIMER_PAIR_46_PENALTY=2.263858
PRIMER_LEFT_46_PENALTY=0.039813
PRIMER_RIGHT_46_PENALTY=2.224046
PRIMER_LEFT_46_SEQUENCE=GCTTTGCAGCATTTGATTGA
PRIMER_RIGHT_46_SEQUENCE=GAAAAAGCTGATGAAGATGGG
PRIMER_LEFT_46=911,20
PRIMER_RIGHT_46=1827,21
PRIMER_LEFT_46_TM=59.960
PRIMER_RIGHT_46_TM=58.776
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=9.6000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=917
PRIMER_PAIR_47_PENALTY=2.268502
PRIMER_LEFT_47_PENALTY=0.044457
PRIMER_RIGHT_47_PENALTY=2.224046
PRIMER_LEFT_47_SEQUENCE=ACGAGGTTGCTATTGCTGCT
PRIMER_RIGHT_47_SEQUENCE=GAAAAAGCTGATGAAGATGGG
PRIMER_LEFT_47=867,20
PRIMER_RIGHT_47=1827,21
PRIMER_LEFT_47_TM=60.044
PRIMER_RIGHT_47_TM=58.776
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=9.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=961
PRIMER_PAIR_48_PENALTY=2.314517
PRIMER_LEFT_48_PENALTY=0.039813
PRIMER_RIGHT_48_PENALTY=2.274705
PRIMER_LEFT_48_SEQUENCE=GCTTTGCAGCATTTGATTGA
PRIMER_RIGHT_48_SEQUENCE=AGAAAAAGCTGATGAAGATGGG
PRIMER_LEFT_48=911,20
PRIMER_RIGHT_48=1828,22
PRIMER_LEFT_48_TM=59.960
PRIMER_RIGHT_48_TM=59.725
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=9.6000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=918
PRIMER_PAIR_49_PENALTY=2.319161
PRIMER_LEFT_49_PENALTY=0.044457
PRIMER_RIGHT_49_PENALTY=2.274705
PRIMER_LEFT_49_SEQUENCE=ACGAGGTTGCTATTGCTGCT
PRIMER_RIGHT_49_SEQUENCE=AGAAAAAGCTGATGAAGATGGG
PRIMER_LEFT_49=867,20
PRIMER_RIGHT_49=1828,22
PRIMER_LEFT_49_TM=60.044
PRIMER_RIGHT_49_TM=59.725
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=9.6000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=962
=
SEQUENCE_ID=MAGIC.433_5_13561670_13561720_hi-0_sf-2_121986_250753_TRUE
SEQUENCE_TARGET=950,150
SEQUENCE_TEMPLATE=CATAAGGCTGTGAANNGTAATTTTAATCTATAAATACACTCATTTTCACTCATTCATTCACCCAAAATTCATATCTCTCCTCATATATTTTCTTTANNTTTTTTAAGTTTTCANGGATTCGCTTATTTTTAATTATTTGCTTAATCGCAATTGTTACTTGTATNTATGGTATCTTCGTTGGGAAATTTACTCNNNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGACTATTTGAGGGTACATCGTACCTCGATGTTTCCAANTTTGTAAACGATTTNGACGAGGCCGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9318243_9318789_po-0_bur-0_124098_16959_TRUE
SEQUENCE_TARGET=950,646
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGCATGTTNACTTTTGACCTTTGACGAAATGTATGAAAGAGAATCTGACGGTCCATATTATTAGCTGATATTGAAACCAAAATNACAGCCGTNCAANATTATCTGCTTTCACACTTAAAGATTATTTAAGAAGNTATACTTAATTGTTTTGTTGCATAGTATTTGTACACGTTATTAGTATATCTTCTGGTTATATTCTTAGATAANNNNTTTTGTAATTTTACCTAATGCAAATCAATAGAGACCTTGAGAAATTGGTAACNATTTAGAGACTAATACTAGNNACACACGTNAAGATTGTCTTTTTGAAACATATCCAAAGTTGATAAAAACTTAATCCCAAAATGACGACATNATATCTCTAANGGAGAAGTCCACAAGATGTCACGAATTTAATAATTATGNAATCAAACNGGAGGGATGATCAGACGAATNACNCCGTTCTTNGCAATGNTCTTGATCTTACGGATGGGAATGTCGAAAGGAGTGGAGAGNATAGCAACNTCNCCAGNGTAGAAACGTCCACTTGAGTCGTGGCTAATNNCAGGNGCTTCACCGGTCACCCTCACTCTCCCATTCATGACTGCGACAGTGAAGTTCTCTNTGGGAACNCCNGGCATGTCTACACGCACGTANAGACCACCGTTTTGNAGCNNCTTGGACTCATAAGNCATCTCNGNTCCATAGTTCACACATGGGTGTGGCATCAATATCCGACCCGTTAATTTCGGATCTGCTCCACATAAAANNNAACNACAACAACAACTATTCCCATTTAGACTAACCAANCTCNNNNNNNNNNACTACTATATATAGTGTTNGATTNCTATNTAATANTNTTGCTATGTATAGCATNNNNNNNNNNANGGGNAANATGNGNGTACCNTTAGGATCCGTGCCATGGGNAAATTTGTGANGACCNTNGCCACGCAGATCTATGACCNTAANNNGAAAAGACAANATTTTNCAAACGTTANTGAAGGNAAAATCAAGACNTTTNTTTNACCCAAANAAAAATGAAGACTTTGTNNNNNNNNNNNAGAGACGATATATCAAAATAGAAAGTTTNGTGAAAACGACANATCGAATAAANTACCTTCTCNGAAATCTGGGCCAGCAAGAAACGCTGTNNAANAACACAAATTCATCAAAAGTTTTTATTACCAAAAAAGNTTCCATAAATNGAAGTGACTGNATAATAAAGGTAATTACAGATGCAGGAGGAGCGTTGTGGAGTGATGNTGGACTTGGTGAGGAGAAGCCTAAGAACACCATCAGACATGAAGGAAGTGAATCCGGAGATTTCACAGCATTTGCAGACGAGTCCGGTGGAGGTACCGTAGTTCCGTTCAGAGTAGTCGTATTTGTGTTCCTTTGGTGCGTCGGNGNAGATACAAACAGCTTTCTTCGACTGGTCAANAAAAACCCTCACACCATCTCTTGGAACGCCGGGAAAATCNATCCGAACGTACATATCTTCGTTNTCCAACATTTTGAACTCCATGAACCCTTTTGGCCCATTTGCTTGAAACTGGTTGTTTATTGCATAAAACCCCTCTNTTCCAATCAACANNAAAAAAAGGAGTAAACTTTGGGTTAAAAGAGCAAATGTANTGTAAGTGAGATTTGAGATTTGGTTTAGAAAGATGTTAGTACCGCGAGATGAAGGAATAGCTCTGAGAGGGACGACCATGATGATTCGACTGAGATTTCTGGTTAGCTACTTTGGGATTAGTTTTTTTCTGNTTTCTCTC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_10482590_10483184_oy-0_tsu-0_6251_1194617_TRUE
SEQUENCE_TARGET=950,694
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNCTTGAACTTTGCAGGATGAGACAAGAAGGTAGAGATCTTTTTGTNAGNNNNNNNNNNNNNNNNNNNTTTNAGAGNTNNNNNNNNNNNAGTTNTTCACACNAGGTGAGNTTTTCCTNNANNNNNNNNNNNNNGTTNTTCACANANNGNGAGANTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNGCATTCATTGCAANCAAACANCNNNNNNNNNNACACAANNNNNNNNNAGTACAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNANNGNNGNNANNCCNTCCTCANNNGAAGAGNGNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_15074380_15074431_mt-0_bur-0_18185_23918_TRUE
SEQUENCE_TARGET=950,151
SEQUENCE_TEMPLATE=ACGCAAGGCTTTCAACAATCAAAGAGTTCGGTGAAAACGTTAAGGCTATTTTTAACCATGCCTATGCAGGANTTTTTTTTTTTACCATGCCTATGCAAGACTTTGAGTAGTGAAAGAGTTCGGTGAAAATTTNATGGCGTTGAAATNGACTGTGACGGGAGCAGCATCAAGAAACTAAANNNGCTACATAGATTCTTCAATAGATAAGGAGAAGAAAATGATAGGTGGCTCAGTGACTCTGATTTTTGAGCCTACAAAATCAACTCTAAACTAAAACCTTGTTAAATGTTAACTCAAAGCCTAAAAGCAACTTCTGATCNTNGNTCNTCAGGTTAAAACTNNNNNNNNNGGCTACTTAANGAAAACANACTTACACCAACCTTCTTACANTTGCTTCAACTCATTNATGCAAAGCNTCATCCCTAACAATNGACCACATAACAAACTGTGAGGGATCAACATGAAACTATCTTTAGGTCTTTGGGAGCNCACTTTAAGNACCTTTTTCACCTTTGGTGATTGGTAAGTCATAGTGACTCCTTCCCANGTNNNNNNNNAGAGACNATATGAGAACTCTCCTACTTCTGGAGNAGATGGTGCAATNCAGCTNACAGNTACATAAANACCACACGGCTCCTCAAAACACTGCACTACAAACAATAGTTTCTTCTNATATNCCCTTTCAATTNNTATCTTATCGCTGATGAACATCATCGCGGCTATATAGNAAACGCCATTGAANCTATAAGCNGTGCTACCTGTANNGTGAGTAAGATCATNGTGCTCGNATAGATCCTTGTATGAGCCAGTATAATTGCAANCTNGTACAGGGCAGGAGCATNNAGAGAAGTTGCATATCTTCTCATGGGTTGATTCTCTCCCATAATAGATTGTCTTGGTGCAACCTAAATCTGCNTATCGGCATNGGACCAAGACNGATTCAAGAACTCTCTCCATTGCTCTACAGCGAATGTGTCCAACAGGCAAAGCACATGCAGGANATTTATTCCTCANTTTGGGACAGCAAGAAGAACAGGCCAAATGCCCATTTNCACACTAAAAATATNNAACCANTGAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACAAAACCTGGAAGACAGGAATCTTCAATGCNTGGTAGCAAATGGGACANTCNAGAATATCAAGATCCGTCAGNATNGCTGAGCGNGNCTTATCAACTNGCTTCCTTCCATCATCGGAAGATATAGAAGAACAGTGTCTCTTTTGAGATCCATTTCNTTCNCTCNANNAAACTCCCACCATGTNGCNTAGAANTCAATAGGAGAGANTGGCTAAACCTTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.149755
PRIMER_LEFT_0_PENALTY=0.137602
PRIMER_RIGHT_0_PENALTY=2.012153
PRIMER_LEFT_0_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_0_SEQUENCE=TCCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_0=862,20
PRIMER_RIGHT_0=1310,22
PRIMER_LEFT_0_TM=59.862
PRIMER_RIGHT_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=449
PRIMER_PAIR_1_PENALTY=2.149755
PRIMER_LEFT_1_PENALTY=0.137602
PRIMER_RIGHT_1_PENALTY=2.012153
PRIMER_LEFT_1_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_1_SEQUENCE=TTCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_1=862,20
PRIMER_RIGHT_1=1311,22
PRIMER_LEFT_1_TM=59.862
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=450
PRIMER_PAIR_2_PENALTY=2.161158
PRIMER_LEFT_2_PENALTY=0.137602
PRIMER_RIGHT_2_PENALTY=2.023556
PRIMER_LEFT_2_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_2_SEQUENCE=GATTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_2=862,20
PRIMER_RIGHT_2=1313,22
PRIMER_LEFT_2_TM=59.862
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=452
PRIMER_PAIR_3_PENALTY=2.243425
PRIMER_LEFT_3_PENALTY=0.231272
PRIMER_RIGHT_3_PENALTY=2.012153
PRIMER_LEFT_3_SEQUENCE=ACACGGCTCCTCAAAACACT
PRIMER_RIGHT_3_SEQUENCE=TCCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_3=627,20
PRIMER_RIGHT_3=1310,22
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=684
PRIMER_PAIR_4_PENALTY=2.247734
PRIMER_LEFT_4_PENALTY=0.137602
PRIMER_RIGHT_4_PENALTY=2.110131
PRIMER_LEFT_4_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_4_SEQUENCE=TGAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_4=862,20
PRIMER_RIGHT_4=1317,22
PRIMER_LEFT_4_TM=59.862
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=456
PRIMER_PAIR_5_PENALTY=2.341403
PRIMER_LEFT_5_PENALTY=0.231272
PRIMER_RIGHT_5_PENALTY=2.110131
PRIMER_LEFT_5_SEQUENCE=ACACGGCTCCTCAAAACACT
PRIMER_RIGHT_5_SEQUENCE=TGAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_5=627,20
PRIMER_RIGHT_5=1317,22
PRIMER_LEFT_5_TM=59.769
PRIMER_RIGHT_5_TM=60.110
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.455
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.1000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=691
PRIMER_PAIR_6_PENALTY=2.367851
PRIMER_LEFT_6_PENALTY=0.137602
PRIMER_RIGHT_6_PENALTY=2.230249
PRIMER_LEFT_6_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_6_SEQUENCE=TTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_6=862,20
PRIMER_RIGHT_6=1311,20
PRIMER_LEFT_6_TM=59.862
PRIMER_RIGHT_6_TM=57.770
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=450
PRIMER_PAIR_7_PENALTY=2.455981
PRIMER_LEFT_7_PENALTY=0.137602
PRIMER_RIGHT_7_PENALTY=2.318379
PRIMER_LEFT_7_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_7_SEQUENCE=TCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_7=862,20
PRIMER_RIGHT_7=1310,21
PRIMER_LEFT_7_TM=59.862
PRIMER_RIGHT_7_TM=58.682
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=449
PRIMER_PAIR_8_PENALTY=2.455981
PRIMER_LEFT_8_PENALTY=0.137602
PRIMER_RIGHT_8_PENALTY=2.318379
PRIMER_LEFT_8_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_8_SEQUENCE=TTCCTGTCTTCCAGGTTTTGT
PRIMER_LEFT_8=862,20
PRIMER_RIGHT_8=1311,21
PRIMER_LEFT_8_TM=59.862
PRIMER_RIGHT_8_TM=58.682
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=450
PRIMER_PAIR_9_PENALTY=2.924084
PRIMER_LEFT_9_PENALTY=0.137602
PRIMER_RIGHT_9_PENALTY=2.786482
PRIMER_LEFT_9_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_9_SEQUENCE=CCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_9=862,20
PRIMER_RIGHT_9=1309,21
PRIMER_LEFT_9_TM=59.862
PRIMER_RIGHT_9_TM=58.214
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=448
PRIMER_PAIR_10_PENALTY=2.939941
PRIMER_LEFT_10_PENALTY=0.137602
PRIMER_RIGHT_10_PENALTY=2.802338
PRIMER_LEFT_10_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_10_SEQUENCE=TCCTGTCTTCCAGGTTTTGT
PRIMER_LEFT_10=862,20
PRIMER_RIGHT_10=1310,20
PRIMER_LEFT_10_TM=59.862
PRIMER_RIGHT_10_TM=57.198
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=449
PRIMER_PAIR_11_PENALTY=2.976570
PRIMER_LEFT_11_PENALTY=0.137602
PRIMER_RIGHT_11_PENALTY=2.838968
PRIMER_LEFT_11_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_11_SEQUENCE=ATTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_11=862,20
PRIMER_RIGHT_11=1312,21
PRIMER_LEFT_11_TM=59.862
PRIMER_RIGHT_11_TM=58.161
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=451
PRIMER_PAIR_12_PENALTY=3.017754
PRIMER_LEFT_12_PENALTY=0.231272
PRIMER_RIGHT_12_PENALTY=2.786482
PRIMER_LEFT_12_SEQUENCE=ACACGGCTCCTCAAAACACT
PRIMER_RIGHT_12_SEQUENCE=CCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_12=627,20
PRIMER_RIGHT_12=1309,21
PRIMER_LEFT_12_TM=59.769
PRIMER_RIGHT_12_TM=58.214
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.1000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=683
PRIMER_PAIR_13_PENALTY=3.126237
PRIMER_LEFT_13_PENALTY=0.137602
PRIMER_RIGHT_13_PENALTY=2.988635
PRIMER_LEFT_13_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_13_SEQUENCE=ATTCCTGTCTTCCAGGTTTTGT
PRIMER_LEFT_13=862,20
PRIMER_RIGHT_13=1312,22
PRIMER_LEFT_13_TM=59.862
PRIMER_RIGHT_13_TM=59.011
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=451
PRIMER_PAIR_14_PENALTY=3.142651
PRIMER_LEFT_14_PENALTY=0.137602
PRIMER_RIGHT_14_PENALTY=3.005048
PRIMER_LEFT_14_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_14_SEQUENCE=GAAGATTCCTGTCTTCCAGGTTT
PRIMER_LEFT_14=862,20
PRIMER_RIGHT_14=1316,23
PRIMER_LEFT_14_TM=59.862
PRIMER_RIGHT_14_TM=59.995
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=455
PRIMER_PAIR_15_PENALTY=3.397858
PRIMER_LEFT_15_PENALTY=0.137602
PRIMER_RIGHT_15_PENALTY=3.260255
PRIMER_LEFT_15_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_15_SEQUENCE=CCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_15=862,20
PRIMER_RIGHT_15=1309,20
PRIMER_LEFT_15_TM=59.862
PRIMER_RIGHT_15_TM=56.740
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=448
PRIMER_PAIR_16_PENALTY=3.423218
PRIMER_LEFT_16_PENALTY=0.137602
PRIMER_RIGHT_16_PENALTY=3.285616
PRIMER_LEFT_16_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_16_SEQUENCE=GAAGATTCCTGTCTTCCAGGTT
PRIMER_LEFT_16=862,20
PRIMER_RIGHT_16=1316,22
PRIMER_LEFT_16_TM=59.862
PRIMER_RIGHT_16_TM=58.714
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.455
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=455
PRIMER_PAIR_17_PENALTY=3.516888
PRIMER_LEFT_17_PENALTY=0.231272
PRIMER_RIGHT_17_PENALTY=3.285616
PRIMER_LEFT_17_SEQUENCE=ACACGGCTCCTCAAAACACT
PRIMER_RIGHT_17_SEQUENCE=GAAGATTCCTGTCTTCCAGGTT
PRIMER_LEFT_17=627,20
PRIMER_RIGHT_17=1316,22
PRIMER_LEFT_17_TM=59.769
PRIMER_RIGHT_17_TM=58.714
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.1000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=690
PRIMER_PAIR_18_PENALTY=3.639501
PRIMER_LEFT_18_PENALTY=1.627348
PRIMER_RIGHT_18_PENALTY=2.012153
PRIMER_LEFT_18_SEQUENCE=TGGGTTGATTCTCTCCCATAA
PRIMER_RIGHT_18_SEQUENCE=TCCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_18=865,21
PRIMER_RIGHT_18=1310,22
PRIMER_LEFT_18_TM=59.373
PRIMER_RIGHT_18_TM=60.012
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=446
PRIMER_PAIR_19_PENALTY=3.639501
PRIMER_LEFT_19_PENALTY=1.627348
PRIMER_RIGHT_19_PENALTY=2.012153
PRIMER_LEFT_19_SEQUENCE=TGGGTTGATTCTCTCCCATAA
PRIMER_RIGHT_19_SEQUENCE=TTCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_19=865,21
PRIMER_RIGHT_19=1311,22
PRIMER_LEFT_19_TM=59.373
PRIMER_RIGHT_19_TM=60.012
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.2000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=447
PRIMER_PAIR_20_PENALTY=3.650904
PRIMER_LEFT_20_PENALTY=1.627348
PRIMER_RIGHT_20_PENALTY=2.023556
PRIMER_LEFT_20_SEQUENCE=TGGGTTGATTCTCTCCCATAA
PRIMER_RIGHT_20_SEQUENCE=GATTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_20=865,21
PRIMER_RIGHT_20=1313,22
PRIMER_LEFT_20_TM=59.373
PRIMER_RIGHT_20_TM=59.976
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.2000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=449
PRIMER_PAIR_21_PENALTY=3.705310
PRIMER_LEFT_21_PENALTY=1.693157
PRIMER_RIGHT_21_PENALTY=2.012153
PRIMER_LEFT_21_SEQUENCE=CACACGGCTCCTCAAAACAC
PRIMER_RIGHT_21_SEQUENCE=TTCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_21=626,20
PRIMER_RIGHT_21=1311,22
PRIMER_LEFT_21_TM=61.693
PRIMER_RIGHT_21_TM=60.012
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=686
PRIMER_PAIR_22_PENALTY=3.737479
PRIMER_LEFT_22_PENALTY=1.627348
PRIMER_RIGHT_22_PENALTY=2.110131
PRIMER_LEFT_22_SEQUENCE=TGGGTTGATTCTCTCCCATAA
PRIMER_RIGHT_22_SEQUENCE=TGAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_22=865,21
PRIMER_RIGHT_22=1317,22
PRIMER_LEFT_22_TM=59.373
PRIMER_RIGHT_22_TM=60.110
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=453
PRIMER_PAIR_23_PENALTY=3.803288
PRIMER_LEFT_23_PENALTY=1.693157
PRIMER_RIGHT_23_PENALTY=2.110131
PRIMER_LEFT_23_SEQUENCE=CACACGGCTCCTCAAAACAC
PRIMER_RIGHT_23_SEQUENCE=TGAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_23=626,20
PRIMER_RIGHT_23=1317,22
PRIMER_LEFT_23_TM=61.693
PRIMER_RIGHT_23_TM=60.110
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=692
PRIMER_PAIR_24_PENALTY=3.845495
PRIMER_LEFT_24_PENALTY=0.137602
PRIMER_RIGHT_24_PENALTY=3.707892
PRIMER_LEFT_24_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_24_SEQUENCE=GAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_24=862,20
PRIMER_RIGHT_24=1316,21
PRIMER_LEFT_24_TM=59.862
PRIMER_RIGHT_24_TM=57.292
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=455
PRIMER_PAIR_25_PENALTY=3.849414
PRIMER_LEFT_25_PENALTY=1.837261
PRIMER_RIGHT_25_PENALTY=2.012153
PRIMER_LEFT_25_SEQUENCE=CTCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_25_SEQUENCE=TCCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_25=861,21
PRIMER_RIGHT_25=1310,22
PRIMER_LEFT_25_TM=60.837
PRIMER_RIGHT_25_TM=60.012
PRIMER_LEFT_25_GC_PERCENT=52.381
PRIMER_RIGHT_25_GC_PERCENT=40.909
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=450
PRIMER_PAIR_26_PENALTY=3.849414
PRIMER_LEFT_26_PENALTY=1.837261
PRIMER_RIGHT_26_PENALTY=2.012153
PRIMER_LEFT_26_SEQUENCE=CTCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_26_SEQUENCE=TTCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_26=861,21
PRIMER_RIGHT_26=1311,22
PRIMER_LEFT_26_TM=60.837
PRIMER_RIGHT_26_TM=60.012
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=40.909
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=451
PRIMER_PAIR_27_PENALTY=3.857596
PRIMER_LEFT_27_PENALTY=1.627348
PRIMER_RIGHT_27_PENALTY=2.230249
PRIMER_LEFT_27_SEQUENCE=TGGGTTGATTCTCTCCCATAA
PRIMER_RIGHT_27_SEQUENCE=TTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_27=865,21
PRIMER_RIGHT_27=1311,20
PRIMER_LEFT_27_TM=59.373
PRIMER_RIGHT_27_TM=57.770
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.2000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=447
PRIMER_PAIR_28_PENALTY=3.860817
PRIMER_LEFT_28_PENALTY=1.837261
PRIMER_RIGHT_28_PENALTY=2.023556
PRIMER_LEFT_28_SEQUENCE=CTCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_28_SEQUENCE=GATTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_28=861,21
PRIMER_RIGHT_28=1313,22
PRIMER_LEFT_28_TM=60.837
PRIMER_RIGHT_28_TM=59.976
PRIMER_LEFT_28_GC_PERCENT=52.381
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.4000
PRIMER_RIGHT_28_END_STABILITY=7.6000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=453
PRIMER_PAIR_29_PENALTY=3.874562
PRIMER_LEFT_29_PENALTY=0.137602
PRIMER_RIGHT_29_PENALTY=3.736960
PRIMER_LEFT_29_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_29_SEQUENCE=AGATTCCTGTCTTCCAGGTTTT
PRIMER_LEFT_29=862,20
PRIMER_RIGHT_29=1314,22
PRIMER_LEFT_29_TM=59.862
PRIMER_RIGHT_29_TM=58.263
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.909
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=453
PRIMER_PAIR_30_PENALTY=3.874562
PRIMER_LEFT_30_PENALTY=0.137602
PRIMER_RIGHT_30_PENALTY=3.736960
PRIMER_LEFT_30_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_30_SEQUENCE=AAGATTCCTGTCTTCCAGGTTT
PRIMER_LEFT_30=862,20
PRIMER_RIGHT_30=1315,22
PRIMER_LEFT_30_TM=59.862
PRIMER_RIGHT_30_TM=58.263
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=454
PRIMER_PAIR_31_PENALTY=3.874595
PRIMER_LEFT_31_PENALTY=0.137602
PRIMER_RIGHT_31_PENALTY=3.736993
PRIMER_LEFT_31_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_31_SEQUENCE=GATTCCTGTCTTCCAGGTTTTGT
PRIMER_LEFT_31=862,20
PRIMER_RIGHT_31=1313,23
PRIMER_LEFT_31_TM=59.862
PRIMER_RIGHT_31_TM=60.737
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=43.478
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=452
PRIMER_PAIR_32_PENALTY=3.896756
PRIMER_LEFT_32_PENALTY=0.137602
PRIMER_RIGHT_32_PENALTY=3.759153
PRIMER_LEFT_32_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_32_SEQUENCE=GATTCCTGTCTTCCAGGTTTT
PRIMER_LEFT_32=862,20
PRIMER_RIGHT_32=1313,21
PRIMER_LEFT_32_TM=59.862
PRIMER_RIGHT_32_TM=57.241
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=452
PRIMER_PAIR_33_PENALTY=3.932991
PRIMER_LEFT_33_PENALTY=1.920838
PRIMER_RIGHT_33_PENALTY=2.012153
PRIMER_LEFT_33_SEQUENCE=TCTCATGGGTTGATTCTCTCC
PRIMER_RIGHT_33_SEQUENCE=TCCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_33=860,21
PRIMER_RIGHT_33=1310,22
PRIMER_LEFT_33_TM=59.079
PRIMER_RIGHT_33_TM=60.012
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=40.909
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=451
PRIMER_PAIR_34_PENALTY=3.932991
PRIMER_LEFT_34_PENALTY=1.920838
PRIMER_RIGHT_34_PENALTY=2.012153
PRIMER_LEFT_34_SEQUENCE=TCTCATGGGTTGATTCTCTCC
PRIMER_RIGHT_34_SEQUENCE=TTCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_34=860,21
PRIMER_RIGHT_34=1311,22
PRIMER_LEFT_34_TM=59.079
PRIMER_RIGHT_34_TM=60.012
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=452
PRIMER_PAIR_35_PENALTY=3.939164
PRIMER_LEFT_35_PENALTY=0.231272
PRIMER_RIGHT_35_PENALTY=3.707892
PRIMER_LEFT_35_SEQUENCE=ACACGGCTCCTCAAAACACT
PRIMER_RIGHT_35_SEQUENCE=GAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_35=627,20
PRIMER_RIGHT_35=1316,21
PRIMER_LEFT_35_TM=59.769
PRIMER_RIGHT_35_TM=57.292
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.1000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=690
PRIMER_PAIR_36_PENALTY=3.944394
PRIMER_LEFT_36_PENALTY=1.920838
PRIMER_RIGHT_36_PENALTY=2.023556
PRIMER_LEFT_36_SEQUENCE=TCTCATGGGTTGATTCTCTCC
PRIMER_RIGHT_36_SEQUENCE=GATTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_36=860,21
PRIMER_RIGHT_36=1313,22
PRIMER_LEFT_36_TM=59.079
PRIMER_RIGHT_36_TM=59.976
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=45.455
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=454
PRIMER_PAIR_37_PENALTY=3.945726
PRIMER_LEFT_37_PENALTY=1.627348
PRIMER_RIGHT_37_PENALTY=2.318379
PRIMER_LEFT_37_SEQUENCE=TGGGTTGATTCTCTCCCATAA
PRIMER_RIGHT_37_SEQUENCE=TCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_37=865,21
PRIMER_RIGHT_37=1310,21
PRIMER_LEFT_37_TM=59.373
PRIMER_RIGHT_37_TM=58.682
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.2000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=446
PRIMER_PAIR_38_PENALTY=3.945726
PRIMER_LEFT_38_PENALTY=1.627348
PRIMER_RIGHT_38_PENALTY=2.318379
PRIMER_LEFT_38_SEQUENCE=TGGGTTGATTCTCTCCCATAA
PRIMER_RIGHT_38_SEQUENCE=TTCCTGTCTTCCAGGTTTTGT
PRIMER_LEFT_38=865,21
PRIMER_RIGHT_38=1311,21
PRIMER_LEFT_38_TM=59.373
PRIMER_RIGHT_38_TM=58.682
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.2000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=447
PRIMER_PAIR_39_PENALTY=3.947392
PRIMER_LEFT_39_PENALTY=1.837261
PRIMER_RIGHT_39_PENALTY=2.110131
PRIMER_LEFT_39_SEQUENCE=CTCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_39_SEQUENCE=TGAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_39=861,21
PRIMER_RIGHT_39=1317,22
PRIMER_LEFT_39_TM=60.837
PRIMER_RIGHT_39_TM=60.110
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=457
PRIMER_PAIR_40_PENALTY=3.990577
PRIMER_LEFT_40_PENALTY=0.137602
PRIMER_RIGHT_40_PENALTY=3.852975
PRIMER_LEFT_40_SEQUENCE=TCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_40_SEQUENCE=AGATTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_40=862,20
PRIMER_RIGHT_40=1314,23
PRIMER_LEFT_40_TM=59.862
PRIMER_RIGHT_40_TM=60.853
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=43.478
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=453
PRIMER_PAIR_41_PENALTY=4.011536
PRIMER_LEFT_41_PENALTY=1.693157
PRIMER_RIGHT_41_PENALTY=2.318379
PRIMER_LEFT_41_SEQUENCE=CACACGGCTCCTCAAAACAC
PRIMER_RIGHT_41_SEQUENCE=TCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_41=626,20
PRIMER_RIGHT_41=1310,21
PRIMER_LEFT_41_TM=61.693
PRIMER_RIGHT_41_TM=58.682
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=685
PRIMER_PAIR_42_PENALTY=4.030970
PRIMER_LEFT_42_PENALTY=1.920838
PRIMER_RIGHT_42_PENALTY=2.110131
PRIMER_LEFT_42_SEQUENCE=TCTCATGGGTTGATTCTCTCC
PRIMER_RIGHT_42_SEQUENCE=TGAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_42=860,21
PRIMER_RIGHT_42=1317,22
PRIMER_LEFT_42_TM=59.079
PRIMER_RIGHT_42_TM=60.110
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=458
PRIMER_PAIR_43_PENALTY=4.067510
PRIMER_LEFT_43_PENALTY=1.837261
PRIMER_RIGHT_43_PENALTY=2.230249
PRIMER_LEFT_43_SEQUENCE=CTCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_43_SEQUENCE=TTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_43=861,21
PRIMER_RIGHT_43=1311,20
PRIMER_LEFT_43_TM=60.837
PRIMER_RIGHT_43_TM=57.770
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=451
PRIMER_PAIR_44_PENALTY=4.120837
PRIMER_LEFT_44_PENALTY=2.108684
PRIMER_RIGHT_44_PENALTY=2.012153
PRIMER_LEFT_44_SEQUENCE=CCACATAACAAACTGTGAGGGA
PRIMER_RIGHT_44_SEQUENCE=TCCTGTCTTCCAGGTTTTGTTT
PRIMER_LEFT_44=433,22
PRIMER_RIGHT_44=1310,22
PRIMER_LEFT_44_TM=59.891
PRIMER_RIGHT_44_TM=60.012
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=878
PRIMER_PAIR_45_PENALTY=4.132240
PRIMER_LEFT_45_PENALTY=2.108684
PRIMER_RIGHT_45_PENALTY=2.023556
PRIMER_LEFT_45_SEQUENCE=CCACATAACAAACTGTGAGGGA
PRIMER_RIGHT_45_SEQUENCE=GATTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_45=433,22
PRIMER_RIGHT_45=1313,22
PRIMER_LEFT_45_TM=59.891
PRIMER_RIGHT_45_TM=59.976
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=45.455
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=881
PRIMER_PAIR_46_PENALTY=4.151087
PRIMER_LEFT_46_PENALTY=1.920838
PRIMER_RIGHT_46_PENALTY=2.230249
PRIMER_LEFT_46_SEQUENCE=TCTCATGGGTTGATTCTCTCC
PRIMER_RIGHT_46_SEQUENCE=TTCCTGTCTTCCAGGTTTTG
PRIMER_LEFT_46=860,21
PRIMER_RIGHT_46=1311,20
PRIMER_LEFT_46_TM=59.079
PRIMER_RIGHT_46_TM=57.770
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=452
PRIMER_PAIR_47_PENALTY=4.155640
PRIMER_LEFT_47_PENALTY=1.837261
PRIMER_RIGHT_47_PENALTY=2.318379
PRIMER_LEFT_47_SEQUENCE=CTCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_47_SEQUENCE=TCCTGTCTTCCAGGTTTTGTT
PRIMER_LEFT_47=861,21
PRIMER_RIGHT_47=1310,21
PRIMER_LEFT_47_TM=60.837
PRIMER_RIGHT_47_TM=58.682
PRIMER_LEFT_47_GC_PERCENT=52.381
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=450
PRIMER_PAIR_48_PENALTY=4.155640
PRIMER_LEFT_48_PENALTY=1.837261
PRIMER_RIGHT_48_PENALTY=2.318379
PRIMER_LEFT_48_SEQUENCE=CTCATGGGTTGATTCTCTCCC
PRIMER_RIGHT_48_SEQUENCE=TTCCTGTCTTCCAGGTTTTGT
PRIMER_LEFT_48=861,21
PRIMER_RIGHT_48=1311,21
PRIMER_LEFT_48_TM=60.837
PRIMER_RIGHT_48_TM=58.682
PRIMER_LEFT_48_GC_PERCENT=52.381
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=451
PRIMER_PAIR_49_PENALTY=4.218815
PRIMER_LEFT_49_PENALTY=2.108684
PRIMER_RIGHT_49_PENALTY=2.110131
PRIMER_LEFT_49_SEQUENCE=CCACATAACAAACTGTGAGGGA
PRIMER_RIGHT_49_SEQUENCE=TGAAGATTCCTGTCTTCCAGGT
PRIMER_LEFT_49=433,22
PRIMER_RIGHT_49=1317,22
PRIMER_LEFT_49_TM=59.891
PRIMER_RIGHT_49_TM=60.110
PRIMER_LEFT_49_GC_PERCENT=45.455
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=885
=
SEQUENCE_ID=MAGIC.149_3_14295471_14295645_ct-1_rsch-4_22031_32465_TRUE
SEQUENCE_TARGET=950,274
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14348825_14348860_zu-0_tsu-0_61782_32249_TRUE
SEQUENCE_TARGET=950,135
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_852823_853068_hi-0_col-0_148586_42030_TRUE
SEQUENCE_TARGET=950,345
SEQUENCE_TEMPLATE=TCANNNNNNNNNNNNNNNNTNTTATTTTGAGNAATGAGAACGTAACATNAGAAGTGTTNTAGTGTTGACGAAATAAAAAGAGAGAGAGGNTTTAGTCTATTTCAAGGCATAAAAAAATGGTTGGTGAAGTGTTGACGAAGGTGGAATACTATAACATGGGCCACGTGGATGACAAANTTACTCCTCGACGTATCNATTAAAGTTGTGGTCAGAAATACAGTACAATTTACCGACTACNTACATGNAANAAGAATATTTTCATTTCATTTCAACTACAGTAGTATAACATTCACGTTATACGATTTTTCATTTTTGTTTTGTAATNAAAGTAATGATTTTCNAAAAAAATCATTGCTATGATNCGAATACATACAGTTTTATATTAGTTTACANATTTATGACAACTATAATACAAAATTTNAATAGTTGTTCAAGGGACGATTGATGTGAACTCGCCAACCATATGCCCTACGTACAAAATAACATATTTACATGTAGAAGTTGAAAATAATAATAATAAAGTGTGATTAAAAACAATTATACAAATGCTAACAATAGGCTANGAGATCGTATATATCTGTAAAGTGTGATTAAAAAACAATTACACAAATGATAATAATAGGCTACTAATCATAATCGTTAAGCTGATTTAATGAAATTATCTTGTGGGCTCAAAGATAAACTGTAAAACCCAATTATATATGTCATATGACGTAAATGATTGATTACACATCTATTTGCACATGCCTAAAGTTGTTCCCCCTTTTGNNNNNNNNNTTGTTGTTATGCCAACTGGCTATAAAGTTAGATGTATTTTACTGTCTGTTATCTCATGTTTTGGAACAAAACCTCTTTAGCTTGATGTCTAACCAAANNTAATTATTTTACTGATTCAAGTGTCTATTCTTTGNNNNNNNNNNNNNNCNTTNNNNNNNNNTTTGTTTGGGAGAATATTGTTATTTGTTAATTGTTATATTAAATATTTTCTTAAANTTTTANANAAATATTTGACAGATNNTTNTTTTTTTTAATTTGCAGTTGCTTTTTAATTCGTTGTGCNCTCATTGTNAATAGTATTTCCCAGTATTATTTAAAAAGNAAAAAGAAGATTCAGTACAGAGTGAATGTTACAGTCTATTACAATAAGATTTNTTTTTGTTGTTCAGAAAAACATAGAACCTAAACGANAAAAAATAATAATACAAAAACTATAGCGATAACAATTATTACTCAATTATAAAAATANAAATCGCAGACCCCATTTTATTAAACTTAAATTTGAAGGTCTCGAGGATGGATCTGTTCTGTAGTGTGTAGTCATCACTAACTTTTAAACTCGTCCTTTTCTGCGCTCGTCCAGTGCCTGCTTCATGATAAAAAAGATTGTTATGAAACTATAAAACTAATTAGTTCAATTTTATAGGTTAGTTTTAGTAATTCTCAAAACATACCTTGCTTTGAAGCATTCTCATCGTCAAGACTAGCGTATCACGCATACTGGTAANAANAAGAAGAAGCCAAAAATCATGAGATTTTAGGTGTGAGTTTGTAAAATAATGTTGCGGTAAAACGAGATACAAAATTACTGTATATGGCTATATGCAGAGAAATGTATATACGTACCGGACATTGAGTGTGCTTATGGGATGTGAAGATCCATCATAACTTATCAGTACAAACTGTGTGGATTCTCCANACGGTACTCTTATCTGCAAAGTTTGTTTAAATTATGATGACCAAGAAAATGAGGAAAGCTTTAATTCTCAATGTCTAAGAATCTTTTTTACCTGAAGTTCCTTTGAGTATTTCTCTGAGATCACTACAGCTTCTGTGGTATTCGTTTTAATCTGGTAACTAGATCTCTTCAAAACAACAGTTGCGGGTTCCCAACTCTCTGATGAATCCATCTTTCGAAATAAATAGCCATGAAAAAACGGTTGTTAAATCAACAGCTGAATTAAACCTGAGACAGTGGATTCAAGATNTGATCTTAAAACTTACCAGAAGCTGCACATTAAAGCTTGCTTGACCTCTGGATATATAAGTGTCGATTTCTGTTTGCATCTCTGTATCTGTAGAAGTAAAAAAACATTCTCAGCAAAGAAGAGATGGTAAATGTGCTCTTCGCTATCTACAAAAAGCAGTATTAGNTTCCGTTGAATACATTACCACATCTAATCTTGTTTTGATCATTAGCAAACAATCTCACTAATTCTCCCTGCATAATCAAAAGAACNTCTTTTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.491637
PRIMER_LEFT_0_PENALTY=0.047584
PRIMER_RIGHT_0_PENALTY=1.444053
PRIMER_LEFT_0_SEQUENCE=TCAAGGGACGATTGATGTGA
PRIMER_RIGHT_0_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_0=430,20
PRIMER_RIGHT_0=1352,20
PRIMER_LEFT_0_TM=60.048
PRIMER_RIGHT_0_TM=61.444
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=923
PRIMER_PAIR_1_PENALTY=1.605867
PRIMER_LEFT_1_PENALTY=0.186949
PRIMER_RIGHT_1_PENALTY=1.418918
PRIMER_LEFT_1_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_1_SEQUENCE=CACATCCCATAAGCACACTCA
PRIMER_LEFT_1=743,20
PRIMER_RIGHT_1=1650,21
PRIMER_LEFT_1_TM=60.187
PRIMER_RIGHT_1_TM=59.581
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=908
PRIMER_PAIR_2_PENALTY=1.631002
PRIMER_LEFT_2_PENALTY=0.186949
PRIMER_RIGHT_2_PENALTY=1.444053
PRIMER_LEFT_2_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_2_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_2=743,20
PRIMER_RIGHT_2=1352,20
PRIMER_LEFT_2_TM=60.187
PRIMER_RIGHT_2_TM=61.444
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=610
PRIMER_PAIR_3_PENALTY=1.845286
PRIMER_LEFT_3_PENALTY=0.047584
PRIMER_RIGHT_3_PENALTY=1.797702
PRIMER_LEFT_3_SEQUENCE=TCAAGGGACGATTGATGTGA
PRIMER_RIGHT_3_SEQUENCE=GCGCAGAAAAGGACGAGTTT
PRIMER_LEFT_3=430,20
PRIMER_RIGHT_3=1351,20
PRIMER_LEFT_3_TM=60.048
PRIMER_RIGHT_3_TM=61.798
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=922
PRIMER_PAIR_4_PENALTY=1.947758
PRIMER_LEFT_4_PENALTY=0.503705
PRIMER_RIGHT_4_PENALTY=1.444053
PRIMER_LEFT_4_SEQUENCE=TTCAAGGGACGATTGATGTG
PRIMER_RIGHT_4_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_4=429,20
PRIMER_RIGHT_4=1352,20
PRIMER_LEFT_4_TM=59.496
PRIMER_RIGHT_4_TM=61.444
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=924
PRIMER_PAIR_5_PENALTY=1.947758
PRIMER_LEFT_5_PENALTY=0.503705
PRIMER_RIGHT_5_PENALTY=1.444053
PRIMER_LEFT_5_SEQUENCE=TGTTCAAGGGACGATTGATG
PRIMER_RIGHT_5_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_5=427,20
PRIMER_RIGHT_5=1352,20
PRIMER_LEFT_5_TM=59.496
PRIMER_RIGHT_5_TM=61.444
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=926
PRIMER_PAIR_6_PENALTY=1.958955
PRIMER_LEFT_6_PENALTY=0.540037
PRIMER_RIGHT_6_PENALTY=1.418918
PRIMER_LEFT_6_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_6_SEQUENCE=CACATCCCATAAGCACACTCA
PRIMER_LEFT_6=745,20
PRIMER_RIGHT_6=1650,21
PRIMER_LEFT_6_TM=59.460
PRIMER_RIGHT_6_TM=59.581
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=906
PRIMER_PAIR_7_PENALTY=1.974340
PRIMER_LEFT_7_PENALTY=0.186949
PRIMER_RIGHT_7_PENALTY=1.787391
PRIMER_LEFT_7_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_7_SEQUENCE=ATCATGAAGCAGGCACTGGA
PRIMER_LEFT_7=743,20
PRIMER_RIGHT_7=1374,20
PRIMER_LEFT_7_TM=60.187
PRIMER_RIGHT_7_TM=61.787
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=12.4000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=632
PRIMER_PAIR_8_PENALTY=1.984090
PRIMER_LEFT_8_PENALTY=0.540037
PRIMER_RIGHT_8_PENALTY=1.444053
PRIMER_LEFT_8_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_8_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_8=745,20
PRIMER_RIGHT_8=1352,20
PRIMER_LEFT_8_TM=59.460
PRIMER_RIGHT_8_TM=61.444
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=608
PRIMER_PAIR_9_PENALTY=1.984651
PRIMER_LEFT_9_PENALTY=0.186949
PRIMER_RIGHT_9_PENALTY=1.797702
PRIMER_LEFT_9_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_9_SEQUENCE=GCGCAGAAAAGGACGAGTTT
PRIMER_LEFT_9=743,20
PRIMER_RIGHT_9=1351,20
PRIMER_LEFT_9_TM=60.187
PRIMER_RIGHT_9_TM=61.798
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=12.4000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=609
PRIMER_PAIR_10_PENALTY=1.989739
PRIMER_LEFT_10_PENALTY=0.570821
PRIMER_RIGHT_10_PENALTY=1.418918
PRIMER_LEFT_10_SEQUENCE=CATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_10_SEQUENCE=CACATCCCATAAGCACACTCA
PRIMER_LEFT_10=742,20
PRIMER_RIGHT_10=1650,21
PRIMER_LEFT_10_TM=59.429
PRIMER_RIGHT_10_TM=59.581
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=10.9000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=909
PRIMER_PAIR_11_PENALTY=2.014874
PRIMER_LEFT_11_PENALTY=0.570821
PRIMER_RIGHT_11_PENALTY=1.444053
PRIMER_LEFT_11_SEQUENCE=CATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_11_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_11=742,20
PRIMER_RIGHT_11=1352,20
PRIMER_LEFT_11_TM=59.429
PRIMER_RIGHT_11_TM=61.444
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.9000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=611
PRIMER_PAIR_12_PENALTY=2.049342
PRIMER_LEFT_12_PENALTY=0.186949
PRIMER_RIGHT_12_PENALTY=1.862393
PRIMER_LEFT_12_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_12_SEQUENCE=TCTTCACATCCCATAAGCACA
PRIMER_LEFT_12=743,20
PRIMER_RIGHT_12=1654,21
PRIMER_LEFT_12_TM=60.187
PRIMER_RIGHT_12_TM=59.138
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=12.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=912
PRIMER_PAIR_13_PENALTY=2.186986
PRIMER_LEFT_13_PENALTY=0.186949
PRIMER_RIGHT_13_PENALTY=2.000037
PRIMER_LEFT_13_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_13_SEQUENCE=TTCACATCCCATAAGCACACTC
PRIMER_LEFT_13=743,20
PRIMER_RIGHT_13=1652,22
PRIMER_LEFT_13_TM=60.187
PRIMER_RIGHT_13_TM=60.000
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=12.4000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=910
PRIMER_PAIR_14_PENALTY=2.186986
PRIMER_LEFT_14_PENALTY=0.186949
PRIMER_RIGHT_14_PENALTY=2.000037
PRIMER_LEFT_14_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_14_SEQUENCE=TCTTCACATCCCATAAGCACAC
PRIMER_LEFT_14=743,20
PRIMER_RIGHT_14=1654,22
PRIMER_LEFT_14_TM=60.187
PRIMER_RIGHT_14_TM=60.000
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=12.4000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=912
PRIMER_PAIR_15_PENALTY=2.264614
PRIMER_LEFT_15_PENALTY=0.845696
PRIMER_RIGHT_15_PENALTY=1.418918
PRIMER_LEFT_15_SEQUENCE=TGCCTAAAGTTGTTCCCCCT
PRIMER_RIGHT_15_SEQUENCE=CACATCCCATAAGCACACTCA
PRIMER_LEFT_15=744,20
PRIMER_RIGHT_15=1650,21
PRIMER_LEFT_15_TM=60.846
PRIMER_RIGHT_15_TM=59.581
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=10.9000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=907
PRIMER_PAIR_16_PENALTY=2.289749
PRIMER_LEFT_16_PENALTY=0.845696
PRIMER_RIGHT_16_PENALTY=1.444053
PRIMER_LEFT_16_SEQUENCE=TGCCTAAAGTTGTTCCCCCT
PRIMER_RIGHT_16_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_16=744,20
PRIMER_RIGHT_16=1352,20
PRIMER_LEFT_16_TM=60.846
PRIMER_RIGHT_16_TM=61.444
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=10.9000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=609
PRIMER_PAIR_17_PENALTY=2.301407
PRIMER_LEFT_17_PENALTY=0.503705
PRIMER_RIGHT_17_PENALTY=1.797702
PRIMER_LEFT_17_SEQUENCE=TTCAAGGGACGATTGATGTG
PRIMER_RIGHT_17_SEQUENCE=GCGCAGAAAAGGACGAGTTT
PRIMER_LEFT_17=429,20
PRIMER_RIGHT_17=1351,20
PRIMER_LEFT_17_TM=59.496
PRIMER_RIGHT_17_TM=61.798
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=923
PRIMER_PAIR_18_PENALTY=2.301407
PRIMER_LEFT_18_PENALTY=0.503705
PRIMER_RIGHT_18_PENALTY=1.797702
PRIMER_LEFT_18_SEQUENCE=TGTTCAAGGGACGATTGATG
PRIMER_RIGHT_18_SEQUENCE=GCGCAGAAAAGGACGAGTTT
PRIMER_LEFT_18=427,20
PRIMER_RIGHT_18=1351,20
PRIMER_LEFT_18_TM=59.496
PRIMER_RIGHT_18_TM=61.798
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=925
PRIMER_PAIR_19_PENALTY=2.327428
PRIMER_LEFT_19_PENALTY=0.540037
PRIMER_RIGHT_19_PENALTY=1.787391
PRIMER_LEFT_19_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_19_SEQUENCE=ATCATGAAGCAGGCACTGGA
PRIMER_LEFT_19=745,20
PRIMER_RIGHT_19=1374,20
PRIMER_LEFT_19_TM=59.460
PRIMER_RIGHT_19_TM=61.787
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=630
PRIMER_PAIR_20_PENALTY=2.337739
PRIMER_LEFT_20_PENALTY=0.540037
PRIMER_RIGHT_20_PENALTY=1.797702
PRIMER_LEFT_20_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_20_SEQUENCE=GCGCAGAAAAGGACGAGTTT
PRIMER_LEFT_20=745,20
PRIMER_RIGHT_20=1351,20
PRIMER_LEFT_20_TM=59.460
PRIMER_RIGHT_20_TM=61.798
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=607
PRIMER_PAIR_21_PENALTY=2.358212
PRIMER_LEFT_21_PENALTY=0.570821
PRIMER_RIGHT_21_PENALTY=1.787391
PRIMER_LEFT_21_SEQUENCE=CATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_21_SEQUENCE=ATCATGAAGCAGGCACTGGA
PRIMER_LEFT_21=742,20
PRIMER_RIGHT_21=1374,20
PRIMER_LEFT_21_TM=59.429
PRIMER_RIGHT_21_TM=61.787
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=10.9000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=633
PRIMER_PAIR_22_PENALTY=2.368523
PRIMER_LEFT_22_PENALTY=0.570821
PRIMER_RIGHT_22_PENALTY=1.797702
PRIMER_LEFT_22_SEQUENCE=CATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_22_SEQUENCE=GCGCAGAAAAGGACGAGTTT
PRIMER_LEFT_22=742,20
PRIMER_RIGHT_22=1351,20
PRIMER_LEFT_22_TM=59.429
PRIMER_RIGHT_22_TM=61.798
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=10.9000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=610
PRIMER_PAIR_23_PENALTY=2.402430
PRIMER_LEFT_23_PENALTY=0.540037
PRIMER_RIGHT_23_PENALTY=1.862393
PRIMER_LEFT_23_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_23_SEQUENCE=TCTTCACATCCCATAAGCACA
PRIMER_LEFT_23=745,20
PRIMER_RIGHT_23=1654,21
PRIMER_LEFT_23_TM=59.460
PRIMER_RIGHT_23_TM=59.138
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=910
PRIMER_PAIR_24_PENALTY=2.433214
PRIMER_LEFT_24_PENALTY=0.570821
PRIMER_RIGHT_24_PENALTY=1.862393
PRIMER_LEFT_24_SEQUENCE=CATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_24_SEQUENCE=TCTTCACATCCCATAAGCACA
PRIMER_LEFT_24=742,20
PRIMER_RIGHT_24=1654,21
PRIMER_LEFT_24_TM=59.429
PRIMER_RIGHT_24_TM=59.138
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=10.9000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=913
PRIMER_PAIR_25_PENALTY=2.462369
PRIMER_LEFT_25_PENALTY=0.047584
PRIMER_RIGHT_25_PENALTY=2.414785
PRIMER_LEFT_25_SEQUENCE=TCAAGGGACGATTGATGTGA
PRIMER_RIGHT_25_SEQUENCE=TCATGAAGCAGGCACTGGAC
PRIMER_LEFT_25=430,20
PRIMER_RIGHT_25=1373,20
PRIMER_LEFT_25_TM=60.048
PRIMER_RIGHT_25_TM=62.415
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=944
PRIMER_PAIR_26_PENALTY=2.540074
PRIMER_LEFT_26_PENALTY=0.540037
PRIMER_RIGHT_26_PENALTY=2.000037
PRIMER_LEFT_26_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_26_SEQUENCE=TTCACATCCCATAAGCACACTC
PRIMER_LEFT_26=745,20
PRIMER_RIGHT_26=1652,22
PRIMER_LEFT_26_TM=59.460
PRIMER_RIGHT_26_TM=60.000
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=908
PRIMER_PAIR_27_PENALTY=2.540074
PRIMER_LEFT_27_PENALTY=0.540037
PRIMER_RIGHT_27_PENALTY=2.000037
PRIMER_LEFT_27_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_27_SEQUENCE=TCTTCACATCCCATAAGCACAC
PRIMER_LEFT_27=745,20
PRIMER_RIGHT_27=1654,22
PRIMER_LEFT_27_TM=59.460
PRIMER_RIGHT_27_TM=60.000
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.455
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=910
PRIMER_PAIR_28_PENALTY=2.570858
PRIMER_LEFT_28_PENALTY=0.570821
PRIMER_RIGHT_28_PENALTY=2.000037
PRIMER_LEFT_28_SEQUENCE=CATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_28_SEQUENCE=TTCACATCCCATAAGCACACTC
PRIMER_LEFT_28=742,20
PRIMER_RIGHT_28=1652,22
PRIMER_LEFT_28_TM=59.429
PRIMER_RIGHT_28_TM=60.000
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=10.9000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=911
PRIMER_PAIR_29_PENALTY=2.570858
PRIMER_LEFT_29_PENALTY=0.570821
PRIMER_RIGHT_29_PENALTY=2.000037
PRIMER_LEFT_29_SEQUENCE=CATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_29_SEQUENCE=TCTTCACATCCCATAAGCACAC
PRIMER_LEFT_29=742,20
PRIMER_RIGHT_29=1654,22
PRIMER_LEFT_29_TM=59.429
PRIMER_RIGHT_29_TM=60.000
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=10.9000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=913
PRIMER_PAIR_30_PENALTY=2.601734
PRIMER_LEFT_30_PENALTY=0.186949
PRIMER_RIGHT_30_PENALTY=2.414785
PRIMER_LEFT_30_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_30_SEQUENCE=TCATGAAGCAGGCACTGGAC
PRIMER_LEFT_30=743,20
PRIMER_RIGHT_30=1373,20
PRIMER_LEFT_30_TM=60.187
PRIMER_RIGHT_30_TM=62.415
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=12.4000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=631
PRIMER_PAIR_31_PENALTY=2.602337
PRIMER_LEFT_31_PENALTY=0.186949
PRIMER_RIGHT_31_PENALTY=2.415388
PRIMER_LEFT_31_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_31_SEQUENCE=TCACATCCCATAAGCACACTC
PRIMER_LEFT_31=743,20
PRIMER_RIGHT_31=1651,21
PRIMER_LEFT_31_TM=60.187
PRIMER_RIGHT_31_TM=58.585
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=12.4000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=909
PRIMER_PAIR_32_PENALTY=2.633087
PRIMER_LEFT_32_PENALTY=0.845696
PRIMER_RIGHT_32_PENALTY=1.787391
PRIMER_LEFT_32_SEQUENCE=TGCCTAAAGTTGTTCCCCCT
PRIMER_RIGHT_32_SEQUENCE=ATCATGAAGCAGGCACTGGA
PRIMER_LEFT_32=744,20
PRIMER_RIGHT_32=1374,20
PRIMER_LEFT_32_TM=60.846
PRIMER_RIGHT_32_TM=61.787
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=10.9000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=631
PRIMER_PAIR_33_PENALTY=2.643398
PRIMER_LEFT_33_PENALTY=0.845696
PRIMER_RIGHT_33_PENALTY=1.797702
PRIMER_LEFT_33_SEQUENCE=TGCCTAAAGTTGTTCCCCCT
PRIMER_RIGHT_33_SEQUENCE=GCGCAGAAAAGGACGAGTTT
PRIMER_LEFT_33=744,20
PRIMER_RIGHT_33=1351,20
PRIMER_LEFT_33_TM=60.846
PRIMER_RIGHT_33_TM=61.798
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=10.9000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=608
PRIMER_PAIR_34_PENALTY=2.653942
PRIMER_LEFT_34_PENALTY=1.235024
PRIMER_RIGHT_34_PENALTY=1.418918
PRIMER_LEFT_34_SEQUENCE=ACATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_34_SEQUENCE=CACATCCCATAAGCACACTCA
PRIMER_LEFT_34=741,21
PRIMER_RIGHT_34=1650,21
PRIMER_LEFT_34_TM=60.235
PRIMER_RIGHT_34_TM=59.581
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=10.9000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=910
PRIMER_PAIR_35_PENALTY=2.679077
PRIMER_LEFT_35_PENALTY=1.235024
PRIMER_RIGHT_35_PENALTY=1.444053
PRIMER_LEFT_35_SEQUENCE=ACATGCCTAAAGTTGTTCCCC
PRIMER_RIGHT_35_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_35=741,21
PRIMER_RIGHT_35=1352,20
PRIMER_LEFT_35_TM=60.235
PRIMER_RIGHT_35_TM=61.444
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=10.9000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=612
PRIMER_PAIR_36_PENALTY=2.708089
PRIMER_LEFT_36_PENALTY=0.845696
PRIMER_RIGHT_36_PENALTY=1.862393
PRIMER_LEFT_36_SEQUENCE=TGCCTAAAGTTGTTCCCCCT
PRIMER_RIGHT_36_SEQUENCE=TCTTCACATCCCATAAGCACA
PRIMER_LEFT_36=744,20
PRIMER_RIGHT_36=1654,21
PRIMER_LEFT_36_TM=60.846
PRIMER_RIGHT_36_TM=59.138
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=911
PRIMER_PAIR_37_PENALTY=2.729646
PRIMER_LEFT_37_PENALTY=0.186949
PRIMER_RIGHT_37_PENALTY=2.542697
PRIMER_LEFT_37_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_37_SEQUENCE=ATCTTCACATCCCATAAGCACA
PRIMER_LEFT_37=743,20
PRIMER_RIGHT_37=1655,22
PRIMER_LEFT_37_TM=60.187
PRIMER_RIGHT_37_TM=59.457
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=12.4000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=913
PRIMER_PAIR_38_PENALTY=2.790283
PRIMER_LEFT_38_PENALTY=1.371364
PRIMER_RIGHT_38_PENALTY=1.418918
PRIMER_LEFT_38_SEQUENCE=GCACATGCCTAAAGTTGTTCC
PRIMER_RIGHT_38_SEQUENCE=CACATCCCATAAGCACACTCA
PRIMER_LEFT_38=739,21
PRIMER_RIGHT_38=1650,21
PRIMER_LEFT_38_TM=59.629
PRIMER_RIGHT_38_TM=59.581
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=912
PRIMER_PAIR_39_PENALTY=2.812503
PRIMER_LEFT_39_PENALTY=1.368450
PRIMER_RIGHT_39_PENALTY=1.444053
PRIMER_LEFT_39_SEQUENCE=GTTCAAGGGACGATTGATGTG
PRIMER_RIGHT_39_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_39=428,21
PRIMER_RIGHT_39=1352,20
PRIMER_LEFT_39_TM=60.368
PRIMER_RIGHT_39_TM=61.444
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=925
PRIMER_PAIR_40_PENALTY=2.815417
PRIMER_LEFT_40_PENALTY=1.371364
PRIMER_RIGHT_40_PENALTY=1.444053
PRIMER_LEFT_40_SEQUENCE=GCACATGCCTAAAGTTGTTCC
PRIMER_RIGHT_40_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_40=739,21
PRIMER_RIGHT_40=1352,20
PRIMER_LEFT_40_TM=59.629
PRIMER_RIGHT_40_TM=61.444
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=614
PRIMER_PAIR_41_PENALTY=2.845733
PRIMER_LEFT_41_PENALTY=0.845696
PRIMER_RIGHT_41_PENALTY=2.000037
PRIMER_LEFT_41_SEQUENCE=TGCCTAAAGTTGTTCCCCCT
PRIMER_RIGHT_41_SEQUENCE=TTCACATCCCATAAGCACACTC
PRIMER_LEFT_41=744,20
PRIMER_RIGHT_41=1652,22
PRIMER_LEFT_41_TM=60.846
PRIMER_RIGHT_41_TM=60.000
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=10.9000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=909
PRIMER_PAIR_42_PENALTY=2.845733
PRIMER_LEFT_42_PENALTY=0.845696
PRIMER_RIGHT_42_PENALTY=2.000037
PRIMER_LEFT_42_SEQUENCE=TGCCTAAAGTTGTTCCCCCT
PRIMER_RIGHT_42_SEQUENCE=TCTTCACATCCCATAAGCACAC
PRIMER_LEFT_42=744,20
PRIMER_RIGHT_42=1654,22
PRIMER_LEFT_42_TM=60.846
PRIMER_RIGHT_42_TM=60.000
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=45.455
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.9000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=911
PRIMER_PAIR_43_PENALTY=2.865669
PRIMER_LEFT_43_PENALTY=1.078278
PRIMER_RIGHT_43_PENALTY=1.787391
PRIMER_LEFT_43_SEQUENCE=ACGATTGATGTGAACTCGCC
PRIMER_RIGHT_43_SEQUENCE=ATCATGAAGCAGGCACTGGA
PRIMER_LEFT_43=437,20
PRIMER_RIGHT_43=1374,20
PRIMER_LEFT_43_TM=61.078
PRIMER_RIGHT_43_TM=61.787
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=938
PRIMER_PAIR_44_PENALTY=2.872371
PRIMER_LEFT_44_PENALTY=1.428318
PRIMER_RIGHT_44_PENALTY=1.444053
PRIMER_LEFT_44_SEQUENCE=ATTGATGTGAACTCGCCAAC
PRIMER_RIGHT_44_SEQUENCE=AGCGCAGAAAAGGACGAGTT
PRIMER_LEFT_44=440,20
PRIMER_RIGHT_44=1352,20
PRIMER_LEFT_44_TM=58.572
PRIMER_RIGHT_44_TM=61.444
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=913
PRIMER_PAIR_45_PENALTY=2.914772
PRIMER_LEFT_45_PENALTY=0.186949
PRIMER_RIGHT_45_PENALTY=2.727823
PRIMER_LEFT_45_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_45_SEQUENCE=TATCATGAAGCAGGCACTGGA
PRIMER_LEFT_45=743,20
PRIMER_RIGHT_45=1375,21
PRIMER_LEFT_45_TM=60.187
PRIMER_RIGHT_45_TM=61.728
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=12.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=633
PRIMER_PAIR_46_PENALTY=2.917626
PRIMER_LEFT_46_PENALTY=0.186949
PRIMER_RIGHT_46_PENALTY=2.730677
PRIMER_LEFT_46_SEQUENCE=ATGCCTAAAGTTGTTCCCCC
PRIMER_RIGHT_46_SEQUENCE=CGCTAGTCTTGACGATGAGAA
PRIMER_LEFT_46=743,20
PRIMER_RIGHT_46=1485,21
PRIMER_LEFT_46_TM=60.187
PRIMER_RIGHT_46_TM=58.269
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=12.4000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=743
PRIMER_PAIR_47_PENALTY=2.934120
PRIMER_LEFT_47_PENALTY=1.515201
PRIMER_RIGHT_47_PENALTY=1.418918
PRIMER_LEFT_47_SEQUENCE=CACATGCCTAAAGTTGTTCCC
PRIMER_RIGHT_47_SEQUENCE=CACATCCCATAAGCACACTCA
PRIMER_LEFT_47=740,21
PRIMER_RIGHT_47=1650,21
PRIMER_LEFT_47_TM=59.485
PRIMER_RIGHT_47_TM=59.581
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=911
PRIMER_PAIR_48_PENALTY=2.954823
PRIMER_LEFT_48_PENALTY=0.540037
PRIMER_RIGHT_48_PENALTY=2.414785
PRIMER_LEFT_48_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_48_SEQUENCE=TCATGAAGCAGGCACTGGAC
PRIMER_LEFT_48=745,20
PRIMER_RIGHT_48=1373,20
PRIMER_LEFT_48_TM=59.460
PRIMER_RIGHT_48_TM=62.415
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=629
PRIMER_PAIR_49_PENALTY=2.955425
PRIMER_LEFT_49_PENALTY=0.540037
PRIMER_RIGHT_49_PENALTY=2.415388
PRIMER_LEFT_49_SEQUENCE=GCCTAAAGTTGTTCCCCCTT
PRIMER_RIGHT_49_SEQUENCE=TCACATCCCATAAGCACACTC
PRIMER_LEFT_49=745,20
PRIMER_RIGHT_49=1651,21
PRIMER_LEFT_49_TM=59.460
PRIMER_RIGHT_49_TM=58.585
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=907
=
SEQUENCE_ID=MAGIC.105_3_14552195_14552566_oy-0_no-0_14009_10272_TRUE
SEQUENCE_TARGET=950,471
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_5920548_5920851_tsu-0_po-0_4266_3759_TRUE
SEQUENCE_TARGET=950,403
SEQUENCE_TEMPLATE=NNNNNNCCAGTNNCNANCTCAAGAAAATGGGAAAATAAANATATTAATTTNANTCCCANTGATAAGTTNATTTCNCNNTTTTNNNNNNANNATTAANATTCCAATAATATAATAATTTCNTTNATANNATTCAGAAATTTNTANNNTTATATTTNTTANCTTTTCAAAAATNNTACATNTTTACNTTGTAGNACTCATCANTTGAATACNCANTTTNTNTNATCTTTAAANAAANTANNATGAGNTTTTGGCTAAACANNAGGAAANNNTGAGAGNGNNNNNNNNNNNTTCCTAANTTGATCGTGTAAATNNTTTGGNCTTATGCAATATATATNGTTNNTGTTTGNNNNNNGNTNNNNTGAAGAGCTTGTATTCAGAAAACTCTTCCAANGNTGTNNNNNNNATNTGTAACATGNTATTAANAACTCTAATGTATTCTAACNNGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGCTGCGAAAGTACTGTTCCGGGNGANGGNATAAGCTGCTATGACANGGAAACACAATGTAGNAATGATTTTGCCAATAATCATTGTAATCCTTATGCANNNAANAACTAAGAAACTTGACGAATAGTTNTACGTACTANNNNNNNNNNNNNNNNNNNNNNNNAATATACANGTTGTAAATATGCATCNGTNTTTACAATATACANCTATGTAATGCAAAATGATAAACCTTCTTTCTCTTNNTTTTTATGTNGTATGTATTTTTCAGATTAATTAATTTAAGANTNNAANTTAATAATTNATATATTCTTTTATTTCAATTTTTGTGAGACTNGNNTTTTNTNATTTCCTACTAANTCCAACCANTATGTACANCTTATANATTTAACTAAATNTANAAATCNACAACTTCAAAACTAGTATTAANAAAAANNNNNNNNNNNNNNNNNNNNNNATAATAATAACAATANNTTTTATNAAAAAATNATNATAATTAGNAATTATGTTAATTTATTNTCTTCANGAATATNTTGCAACATTTAANANTTTTNATAAAATCAAATAAGNGTATTTCTTATCTTTGTCTTNAAAACTTCNNNTCAANAAATNNAAATGATTNNNNNNNNNNNNNNNNNNNNNNNNNANGTGAAAAAGTNGGTTTCTTTCTTAANTTTTATANTACAAAGAATTAAANNTTGAATTTATCACACNAAAAAATAATGACCAGTANTCAACANCATGATTCANTTAAGTATTTAACTATTAAGACTCACCGGAATNATNGNTGGCTCTACTNAAGAACTNTCCAAATCTAAAAACNNTCGTCTTNNNNNCGANATNTTTTTACTNCATATATTGAACATATTTAACTAGACACAANTAGATNTTGATGATGNTTNNNCANTAATTTANGGACTCCATCNNAGAGATACAAGTAAATGTNNAGANGTTGATNGGTGCNTTNGAAAATATTCNGATGAAATTCCTANNTGANTATCNTCAAGTCNGGTGAAGNTTGTNNAAAGN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_3_19404954_19405256_po-0_can-0_145100_8984_TRUE
SEQUENCE_TARGET=950,402
SEQUENCE_TEMPLATE=AGATACGCNAACAAACAACTTGGTGATGAGGGTTTAAAGAAACGGTTGGTGANGTATCGAGAGGTGATAAGGGAAAGCCAGAGCTTTGAGACGCACTTGAATCTCATCAGAGATTTCGCCGGNAATCTGATTAGGATATCGATCAACATTTCTTCAGGGATCTCTGGTAAGAACACCACGGTCTTCTTCTGCTTCTGCTTTTCCTTTTTCCTCACGGCAACACATAAAGACGAGCCCATATTTGTTCGCAGAAGAATAGAGAAGGNGCNATAGAGTACANTAAGGAAAAAAAAACTCGAACATTTCGTTTCCCTTAAAAGGTTGCATCTCCAACTTGTTCTCTCTGTTTGTATGTTGCGGCTCTCACAAGCTAANGCTGCTGCATATTGTGTAAACCCTTGCTCTCTCTTTCCTACGCGGAAAATGATTTCCATTTGTAATTGGGCTTGTTCTCAGNTTTTGGCCCAACTGAATTCAATGGGTTTAGATATAGGCTNAAAAAACTGCATGCAGGTCCAAGAGANATATTGACTGGAANCCAAATATAGAGATATTGGTAANTAATTAATCAATCCAATGNTCTCTATGAATTCCTCAAGAAAAGGATCATTGTTTCTAGGACTCGATGTGGAAAGCATAAAATATAAGCACTTAACCTTCAGCTGAGTGGCCAACAGCGTTACAAGGTAAAGAGAAAGTTACTNATTTTAGAAACATTAAGATCATACCCTGATCCAAGAAAACATTCCAGTTGTTTCCACTTTCGAGACATTACATATCCAAGAAATAGCAGGGCTTGTAGTCGATAGCATGGACAGAGATCGAATACAAGAGGCCAAAGATTTTCTTGACATGGTGGTTGATGAGGTAACAAANTTTTCAACCTGTCNTTTAANNATTGATGAGCAATAAGTCANATAATNTAANGTTGTATAACAGATACAAGNNATTGAAANTCCATTTAATAATNTGGAATGTAAAAACAAAATGACAAACTATANTTAGGTTTCCAAGTACATGATGNTCTCAGTATCATCCCATAAAGTAGCCANNAAGGAATCAGTCTGTTTAAAGCTACCAAGTAATGATGGACTTATTTGGATCTTTCGAGTTATCTCATTACCCTCCAAATCATAANTATAAAAGTGTAGGGGTTGATTCCACGAGAGATTTGCCNTTCTANGCCAAACCTTACCACTGCGACCTGTGCCACCCATCATAAAGCCATAGTCGTGGGGGAAATCTGTAGGCAAAACAAAAGTCTNCTTGTCTGACCATTGACTCTTGTTGACATCTTNAATTNCNCAAACACCCACACTACAATCAGNNAAAACAGTAACATAGTTAAAAACAGCTATTTTTCCTTTNTAGTTCATTAGACTTGGCCAANGGGATCTCTGGAGTNNGTNAACTTTATAGGGTAATTNAATCAAATTGAACTCTTCAGAAGTCAAGTCAAAACTCATAAGCACACANTTGTCATCAGTCNAANCTCCATAATACAAAACACCGTTGANGGTAATCCCNTGANAATAAGGAAGATGGTGAATGTGGCATTGAGTCTTTTTCCATGAAGNTCNAGCTCCAAGTACCAACACATGNTGTTCTGATCTCACTTTCCACTCCTTGTTGTGCCTCCAAATGAAACTAAGCACTTTGTATTCGTCATGAACAGGATCGTGTCCTAAATAGTACCATATCTTGTAATNTTCTCCTCCTTTAGNTGTTAGAATNATCGGCNANTCCACAAGCTGCCTTGTGTTAAGGTTACAAATTTGCACTCTTGACCNAATTGTGAAACACACCAAGCCACGACAAGCGTTNAAGAAGTAGCCTCCCATTATCGGCATGGTTAGATCTTGGTCGATTACAGAGACAGANTGATCATCATGACTCNATGATGTAGTNAGCAACGCATAATTGCTCTGTTTTGCCCTAACNACTAAATAAGCANACAAACTTCGACGTGATGAGCAAGATTTAAAGAAACGGTTGGTCAAGTATCGAGATNTGATCGTGGAAAACCAGATCTTTGAGACGCACTTAAATCTCATCAGANGTTCACCGGTAATCTGCTCAGAATCTCGNTCAANAATTCCTCACAGAGTTCGGGTACGACCACTATTCTCTCCTTCTTTNGCTTTTTCCTCACGGCAAGACCTAAAGACGATAGGTNACCTAGGTTANTGNATCTTGTTTTCNTTTTCCCTTNCCTCAAATNTTTTACGACGGNGNCACATGAANACGACACCATAGTTGNTTCACAGANTTGTATCTCCAAAGCCTANGTCAATCGATCGAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.466368
PRIMER_LEFT_0_PENALTY=0.378122
PRIMER_RIGHT_0_PENALTY=0.088247
PRIMER_LEFT_0_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_0_SEQUENCE=TTTCATTTGGAGGCACAACA
PRIMER_LEFT_0=848,20
PRIMER_RIGHT_0=1646,20
PRIMER_LEFT_0_TM=60.378
PRIMER_RIGHT_0_TM=60.088
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=799
PRIMER_PAIR_1_PENALTY=0.686843
PRIMER_LEFT_1_PENALTY=0.378122
PRIMER_RIGHT_1_PENALTY=0.308722
PRIMER_LEFT_1_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_1_SEQUENCE=TCATTTGGAGGCACAACAAG
PRIMER_LEFT_1=848,20
PRIMER_RIGHT_1=1644,20
PRIMER_LEFT_1_TM=60.378
PRIMER_RIGHT_1_TM=59.691
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=797
PRIMER_PAIR_2_PENALTY=0.817492
PRIMER_LEFT_2_PENALTY=0.378122
PRIMER_RIGHT_2_PENALTY=0.439370
PRIMER_LEFT_2_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_2_SEQUENCE=GCCGATAATGGGAGGCTACT
PRIMER_LEFT_2=848,20
PRIMER_RIGHT_2=1847,20
PRIMER_LEFT_2_TM=60.378
PRIMER_RIGHT_2_TM=60.439
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=1000
PRIMER_PAIR_3_PENALTY=0.834137
PRIMER_LEFT_3_PENALTY=0.801192
PRIMER_RIGHT_3_PENALTY=0.032945
PRIMER_LEFT_3_SEQUENCE=AGGACTCGATGTGGAAAGCA
PRIMER_RIGHT_3_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_3=617,20
PRIMER_RIGHT_3=1561,20
PRIMER_LEFT_3_TM=60.801
PRIMER_RIGHT_3_TM=59.967
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=945
PRIMER_PAIR_4_PENALTY=0.867454
PRIMER_LEFT_4_PENALTY=0.801192
PRIMER_RIGHT_4_PENALTY=0.066262
PRIMER_LEFT_4_SEQUENCE=AGGACTCGATGTGGAAAGCA
PRIMER_RIGHT_4_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_4=617,20
PRIMER_RIGHT_4=1554,20
PRIMER_LEFT_4_TM=60.801
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=938
PRIMER_PAIR_5_PENALTY=0.873294
PRIMER_LEFT_5_PENALTY=0.378122
PRIMER_RIGHT_5_PENALTY=0.495173
PRIMER_LEFT_5_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_5_SEQUENCE=ATTTGGAGGCACAACAAGGA
PRIMER_LEFT_5=848,20
PRIMER_RIGHT_5=1642,20
PRIMER_LEFT_5_TM=60.378
PRIMER_RIGHT_5_TM=60.495
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=795
PRIMER_PAIR_6_PENALTY=1.067803
PRIMER_LEFT_6_PENALTY=0.378122
PRIMER_RIGHT_6_PENALTY=0.689681
PRIMER_LEFT_6_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_6_SEQUENCE=AGGCACAACAAGGAGTGGAA
PRIMER_LEFT_6=848,20
PRIMER_RIGHT_6=1636,20
PRIMER_LEFT_6_TM=60.378
PRIMER_RIGHT_6_TM=60.690
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=789
PRIMER_PAIR_7_PENALTY=1.067803
PRIMER_LEFT_7_PENALTY=0.378122
PRIMER_RIGHT_7_PENALTY=0.689681
PRIMER_LEFT_7_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_7_SEQUENCE=TTGGAGGCACAACAAGGAGT
PRIMER_LEFT_7=848,20
PRIMER_RIGHT_7=1640,20
PRIMER_LEFT_7_TM=60.378
PRIMER_RIGHT_7_TM=60.690
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=793
PRIMER_PAIR_8_PENALTY=1.105263
PRIMER_LEFT_8_PENALTY=1.072318
PRIMER_RIGHT_8_PENALTY=0.032945
PRIMER_LEFT_8_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_8_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_8=778,20
PRIMER_RIGHT_8=1561,20
PRIMER_LEFT_8_TM=58.928
PRIMER_RIGHT_8_TM=59.967
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=784
PRIMER_PAIR_9_PENALTY=1.138580
PRIMER_LEFT_9_PENALTY=1.072318
PRIMER_RIGHT_9_PENALTY=0.066262
PRIMER_LEFT_9_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_9_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_9=778,20
PRIMER_RIGHT_9=1554,20
PRIMER_LEFT_9_TM=58.928
PRIMER_RIGHT_9_TM=59.934
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=777
PRIMER_PAIR_10_PENALTY=1.139871
PRIMER_LEFT_10_PENALTY=0.801192
PRIMER_RIGHT_10_PENALTY=0.338680
PRIMER_LEFT_10_SEQUENCE=AGGACTCGATGTGGAAAGCA
PRIMER_RIGHT_10_SEQUENCE=TCAATGCCACATTCACCATC
PRIMER_LEFT_10=617,20
PRIMER_RIGHT_10=1559,20
PRIMER_LEFT_10_TM=60.801
PRIMER_RIGHT_10_TM=60.339
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=943
PRIMER_PAIR_11_PENALTY=1.160565
PRIMER_LEFT_11_PENALTY=1.072318
PRIMER_RIGHT_11_PENALTY=0.088247
PRIMER_LEFT_11_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_11_SEQUENCE=TTTCATTTGGAGGCACAACA
PRIMER_LEFT_11=778,20
PRIMER_RIGHT_11=1646,20
PRIMER_LEFT_11_TM=58.928
PRIMER_RIGHT_11_TM=60.088
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=869
PRIMER_PAIR_12_PENALTY=1.201966
PRIMER_LEFT_12_PENALTY=1.169021
PRIMER_RIGHT_12_PENALTY=0.032945
PRIMER_LEFT_12_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_12_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_12=821,21
PRIMER_RIGHT_12=1561,20
PRIMER_LEFT_12_TM=59.831
PRIMER_RIGHT_12_TM=59.967
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=741
PRIMER_PAIR_13_PENALTY=1.235283
PRIMER_LEFT_13_PENALTY=1.169021
PRIMER_RIGHT_13_PENALTY=0.066262
PRIMER_LEFT_13_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_13_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_13=821,21
PRIMER_RIGHT_13=1554,20
PRIMER_LEFT_13_TM=59.831
PRIMER_RIGHT_13_TM=59.934
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=734
PRIMER_PAIR_14_PENALTY=1.257268
PRIMER_LEFT_14_PENALTY=1.169021
PRIMER_RIGHT_14_PENALTY=0.088247
PRIMER_LEFT_14_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_14_SEQUENCE=TTTCATTTGGAGGCACAACA
PRIMER_LEFT_14=821,21
PRIMER_RIGHT_14=1646,20
PRIMER_LEFT_14_TM=59.831
PRIMER_RIGHT_14_TM=60.088
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=826
PRIMER_PAIR_15_PENALTY=1.381040
PRIMER_LEFT_15_PENALTY=1.072318
PRIMER_RIGHT_15_PENALTY=0.308722
PRIMER_LEFT_15_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_15_SEQUENCE=TCATTTGGAGGCACAACAAG
PRIMER_LEFT_15=778,20
PRIMER_RIGHT_15=1644,20
PRIMER_LEFT_15_TM=58.928
PRIMER_RIGHT_15_TM=59.691
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=867
PRIMER_PAIR_16_PENALTY=1.410998
PRIMER_LEFT_16_PENALTY=1.072318
PRIMER_RIGHT_16_PENALTY=0.338680
PRIMER_LEFT_16_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_16_SEQUENCE=TCAATGCCACATTCACCATC
PRIMER_LEFT_16=778,20
PRIMER_RIGHT_16=1559,20
PRIMER_LEFT_16_TM=58.928
PRIMER_RIGHT_16_TM=60.339
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=782
PRIMER_PAIR_17_PENALTY=1.427488
PRIMER_LEFT_17_PENALTY=0.801192
PRIMER_RIGHT_17_PENALTY=0.626296
PRIMER_LEFT_17_SEQUENCE=AGGACTCGATGTGGAAAGCA
PRIMER_RIGHT_17_SEQUENCE=CAATGCCACATTCACCATCT
PRIMER_LEFT_17=617,20
PRIMER_RIGHT_17=1558,20
PRIMER_LEFT_17_TM=60.801
PRIMER_RIGHT_17_TM=59.374
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=942
PRIMER_PAIR_18_PENALTY=1.453520
PRIMER_LEFT_18_PENALTY=0.378122
PRIMER_RIGHT_18_PENALTY=1.075398
PRIMER_LEFT_18_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_18_SEQUENCE=GGCACAACAAGGAGTGGAAA
PRIMER_LEFT_18=848,20
PRIMER_RIGHT_18=1635,20
PRIMER_LEFT_18_TM=60.378
PRIMER_RIGHT_18_TM=61.075
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=788
PRIMER_PAIR_19_PENALTY=1.477743
PRIMER_LEFT_19_PENALTY=1.169021
PRIMER_RIGHT_19_PENALTY=0.308722
PRIMER_LEFT_19_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_19_SEQUENCE=TCATTTGGAGGCACAACAAG
PRIMER_LEFT_19=821,21
PRIMER_RIGHT_19=1644,20
PRIMER_LEFT_19_TM=59.831
PRIMER_RIGHT_19_TM=59.691
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=824
PRIMER_PAIR_20_PENALTY=1.481226
PRIMER_LEFT_20_PENALTY=0.378122
PRIMER_RIGHT_20_PENALTY=1.103105
PRIMER_LEFT_20_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_20_SEQUENCE=GGAAAAAGACTCAATGCCACA
PRIMER_LEFT_20=848,20
PRIMER_RIGHT_20=1569,21
PRIMER_LEFT_20_TM=60.378
PRIMER_RIGHT_20_TM=60.103
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=722
PRIMER_PAIR_21_PENALTY=1.481226
PRIMER_LEFT_21_PENALTY=0.378122
PRIMER_RIGHT_21_PENALTY=1.103105
PRIMER_LEFT_21_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_21_SEQUENCE=TGGAAAAAGACTCAATGCCAC
PRIMER_LEFT_21=848,20
PRIMER_RIGHT_21=1570,21
PRIMER_LEFT_21_TM=60.378
PRIMER_RIGHT_21_TM=60.103
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=723
PRIMER_PAIR_22_PENALTY=1.507700
PRIMER_LEFT_22_PENALTY=1.169021
PRIMER_RIGHT_22_PENALTY=0.338680
PRIMER_LEFT_22_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_22_SEQUENCE=TCAATGCCACATTCACCATC
PRIMER_LEFT_22=821,21
PRIMER_RIGHT_22=1559,20
PRIMER_LEFT_22_TM=59.831
PRIMER_RIGHT_22_TM=60.339
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=739
PRIMER_PAIR_23_PENALTY=1.567491
PRIMER_LEFT_23_PENALTY=1.072318
PRIMER_RIGHT_23_PENALTY=0.495173
PRIMER_LEFT_23_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_23_SEQUENCE=ATTTGGAGGCACAACAAGGA
PRIMER_LEFT_23=778,20
PRIMER_RIGHT_23=1642,20
PRIMER_LEFT_23_TM=58.928
PRIMER_RIGHT_23_TM=60.495
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=865
PRIMER_PAIR_24_PENALTY=1.582023
PRIMER_LEFT_24_PENALTY=0.378122
PRIMER_RIGHT_24_PENALTY=1.203901
PRIMER_LEFT_24_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_24_SEQUENCE=TTTGGAGGCACAACAAGGAG
PRIMER_LEFT_24=848,20
PRIMER_RIGHT_24=1641,20
PRIMER_LEFT_24_TM=60.378
PRIMER_RIGHT_24_TM=61.204
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=794
PRIMER_PAIR_25_PENALTY=1.636839
PRIMER_LEFT_25_PENALTY=0.378122
PRIMER_RIGHT_25_PENALTY=1.258717
PRIMER_LEFT_25_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_25_SEQUENCE=GAGGCACAACAAGGAGTGGA
PRIMER_LEFT_25=848,20
PRIMER_RIGHT_25=1637,20
PRIMER_LEFT_25_TM=60.378
PRIMER_RIGHT_25_TM=61.259
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=790
PRIMER_PAIR_26_PENALTY=1.650432
PRIMER_LEFT_26_PENALTY=1.617487
PRIMER_RIGHT_26_PENALTY=0.032945
PRIMER_LEFT_26_SEQUENCE=CCAAGAAATAGCAGGGCTTGT
PRIMER_RIGHT_26_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_26=779,21
PRIMER_RIGHT_26=1561,20
PRIMER_LEFT_26_TM=60.617
PRIMER_RIGHT_26_TM=59.967
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=783
PRIMER_PAIR_27_PENALTY=1.661643
PRIMER_LEFT_27_PENALTY=1.595381
PRIMER_RIGHT_27_PENALTY=0.066262
PRIMER_LEFT_27_SEQUENCE=TTCTTGACATGGTGGTTGATG
PRIMER_RIGHT_27_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_27=844,21
PRIMER_RIGHT_27=1554,20
PRIMER_LEFT_27_TM=59.405
PRIMER_RIGHT_27_TM=59.934
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=6.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=711
PRIMER_PAIR_28_PENALTY=1.664194
PRIMER_LEFT_28_PENALTY=1.169021
PRIMER_RIGHT_28_PENALTY=0.495173
PRIMER_LEFT_28_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_28_SEQUENCE=ATTTGGAGGCACAACAAGGA
PRIMER_LEFT_28=821,21
PRIMER_RIGHT_28=1642,20
PRIMER_LEFT_28_TM=59.831
PRIMER_RIGHT_28_TM=60.495
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=822
PRIMER_PAIR_29_PENALTY=1.683627
PRIMER_LEFT_29_PENALTY=1.595381
PRIMER_RIGHT_29_PENALTY=0.088247
PRIMER_LEFT_29_SEQUENCE=TTCTTGACATGGTGGTTGATG
PRIMER_RIGHT_29_SEQUENCE=TTTCATTTGGAGGCACAACA
PRIMER_LEFT_29=844,21
PRIMER_RIGHT_29=1646,20
PRIMER_LEFT_29_TM=59.405
PRIMER_RIGHT_29_TM=60.088
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=803
PRIMER_PAIR_30_PENALTY=1.683749
PRIMER_LEFT_30_PENALTY=1.617487
PRIMER_RIGHT_30_PENALTY=0.066262
PRIMER_LEFT_30_SEQUENCE=CCAAGAAATAGCAGGGCTTGT
PRIMER_RIGHT_30_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_30=779,21
PRIMER_RIGHT_30=1554,20
PRIMER_LEFT_30_TM=60.617
PRIMER_RIGHT_30_TM=59.934
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=776
PRIMER_PAIR_31_PENALTY=1.687022
PRIMER_LEFT_31_PENALTY=1.654077
PRIMER_RIGHT_31_PENALTY=0.032945
PRIMER_LEFT_31_SEQUENCE=GTGGCCAACAGCGTTACAAG
PRIMER_RIGHT_31_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_31=666,20
PRIMER_RIGHT_31=1561,20
PRIMER_LEFT_31_TM=61.654
PRIMER_RIGHT_31_TM=59.967
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=896
PRIMER_PAIR_32_PENALTY=1.698615
PRIMER_LEFT_32_PENALTY=1.072318
PRIMER_RIGHT_32_PENALTY=0.626296
PRIMER_LEFT_32_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_32_SEQUENCE=CAATGCCACATTCACCATCT
PRIMER_LEFT_32=778,20
PRIMER_RIGHT_32=1558,20
PRIMER_LEFT_32_TM=58.928
PRIMER_RIGHT_32_TM=59.374
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=6.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=781
PRIMER_PAIR_33_PENALTY=1.705733
PRIMER_LEFT_33_PENALTY=1.617487
PRIMER_RIGHT_33_PENALTY=0.088247
PRIMER_LEFT_33_SEQUENCE=CCAAGAAATAGCAGGGCTTGT
PRIMER_RIGHT_33_SEQUENCE=TTTCATTTGGAGGCACAACA
PRIMER_LEFT_33=779,21
PRIMER_RIGHT_33=1646,20
PRIMER_LEFT_33_TM=60.617
PRIMER_RIGHT_33_TM=60.088
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=868
PRIMER_PAIR_34_PENALTY=1.720339
PRIMER_LEFT_34_PENALTY=1.654077
PRIMER_RIGHT_34_PENALTY=0.066262
PRIMER_LEFT_34_SEQUENCE=GTGGCCAACAGCGTTACAAG
PRIMER_RIGHT_34_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_34=666,20
PRIMER_RIGHT_34=1554,20
PRIMER_LEFT_34_TM=61.654
PRIMER_RIGHT_34_TM=59.934
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=889
PRIMER_PAIR_35_PENALTY=1.742324
PRIMER_LEFT_35_PENALTY=1.654077
PRIMER_RIGHT_35_PENALTY=0.088247
PRIMER_LEFT_35_SEQUENCE=GTGGCCAACAGCGTTACAAG
PRIMER_RIGHT_35_SEQUENCE=TTTCATTTGGAGGCACAACA
PRIMER_LEFT_35=666,20
PRIMER_RIGHT_35=1646,20
PRIMER_LEFT_35_TM=61.654
PRIMER_RIGHT_35_TM=60.088
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=981
PRIMER_PAIR_36_PENALTY=1.761999
PRIMER_LEFT_36_PENALTY=1.072318
PRIMER_RIGHT_36_PENALTY=0.689681
PRIMER_LEFT_36_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_36_SEQUENCE=AGGCACAACAAGGAGTGGAA
PRIMER_LEFT_36=778,20
PRIMER_RIGHT_36=1636,20
PRIMER_LEFT_36_TM=58.928
PRIMER_RIGHT_36_TM=60.690
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=859
PRIMER_PAIR_37_PENALTY=1.761999
PRIMER_LEFT_37_PENALTY=1.072318
PRIMER_RIGHT_37_PENALTY=0.689681
PRIMER_LEFT_37_SEQUENCE=TCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_37_SEQUENCE=TTGGAGGCACAACAAGGAGT
PRIMER_LEFT_37=778,20
PRIMER_RIGHT_37=1640,20
PRIMER_LEFT_37_TM=58.928
PRIMER_RIGHT_37_TM=60.690
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=863
PRIMER_PAIR_38_PENALTY=1.795317
PRIMER_LEFT_38_PENALTY=1.169021
PRIMER_RIGHT_38_PENALTY=0.626296
PRIMER_LEFT_38_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_38_SEQUENCE=CAATGCCACATTCACCATCT
PRIMER_LEFT_38=821,21
PRIMER_RIGHT_38=1558,20
PRIMER_LEFT_38_TM=59.831
PRIMER_RIGHT_38_TM=59.374
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=738
PRIMER_PAIR_39_PENALTY=1.796633
PRIMER_LEFT_39_PENALTY=1.763688
PRIMER_RIGHT_39_PENALTY=0.032945
PRIMER_LEFT_39_SEQUENCE=ATCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_39_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_39=777,21
PRIMER_RIGHT_39=1561,20
PRIMER_LEFT_39_TM=59.236
PRIMER_RIGHT_39_TM=59.967
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=785
PRIMER_PAIR_40_PENALTY=1.812489
PRIMER_LEFT_40_PENALTY=0.801192
PRIMER_RIGHT_40_PENALTY=1.011297
PRIMER_LEFT_40_SEQUENCE=AGGACTCGATGTGGAAAGCA
PRIMER_RIGHT_40_SEQUENCE=AGACTCAATGCCACATTCACC
PRIMER_LEFT_40=617,20
PRIMER_RIGHT_40=1563,21
PRIMER_LEFT_40_TM=60.801
PRIMER_RIGHT_40_TM=59.989
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=947
PRIMER_PAIR_41_PENALTY=1.813899
PRIMER_LEFT_41_PENALTY=1.780954
PRIMER_RIGHT_41_PENALTY=0.032945
PRIMER_LEFT_41_SEQUENCE=TAGGACTCGATGTGGAAAGCA
PRIMER_RIGHT_41_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_41=616,21
PRIMER_RIGHT_41=1561,20
PRIMER_LEFT_41_TM=60.781
PRIMER_RIGHT_41_TM=59.967
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=946
PRIMER_PAIR_42_PENALTY=1.818059
PRIMER_LEFT_42_PENALTY=0.378122
PRIMER_RIGHT_42_PENALTY=1.439937
PRIMER_LEFT_42_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_42_SEQUENCE=CATGGAAAAAGACTCAATGCC
PRIMER_LEFT_42=848,20
PRIMER_RIGHT_42=1572,21
PRIMER_LEFT_42_TM=60.378
PRIMER_RIGHT_42_TM=59.560
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=725
PRIMER_PAIR_43_PENALTY=1.829951
PRIMER_LEFT_43_PENALTY=1.763688
PRIMER_RIGHT_43_PENALTY=0.066262
PRIMER_LEFT_43_SEQUENCE=ATCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_43_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_43=777,21
PRIMER_RIGHT_43=1554,20
PRIMER_LEFT_43_TM=59.236
PRIMER_RIGHT_43_TM=59.934
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=778
PRIMER_PAIR_44_PENALTY=1.839584
PRIMER_LEFT_44_PENALTY=0.378122
PRIMER_RIGHT_44_PENALTY=1.461462
PRIMER_LEFT_44_SEQUENCE=TGACATGGTGGTTGATGAGG
PRIMER_RIGHT_44_SEQUENCE=CATTTGGAGGCACAACAAGG
PRIMER_LEFT_44=848,20
PRIMER_RIGHT_44=1643,20
PRIMER_LEFT_44_TM=60.378
PRIMER_RIGHT_44_TM=61.461
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=796
PRIMER_PAIR_45_PENALTY=1.847216
PRIMER_LEFT_45_PENALTY=1.780954
PRIMER_RIGHT_45_PENALTY=0.066262
PRIMER_LEFT_45_SEQUENCE=TAGGACTCGATGTGGAAAGCA
PRIMER_RIGHT_45_SEQUENCE=GCCACATTCACCATCTTCCT
PRIMER_LEFT_45=616,21
PRIMER_RIGHT_45=1554,20
PRIMER_LEFT_45_TM=60.781
PRIMER_RIGHT_45_TM=59.934
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=939
PRIMER_PAIR_46_PENALTY=1.851935
PRIMER_LEFT_46_PENALTY=1.763688
PRIMER_RIGHT_46_PENALTY=0.088247
PRIMER_LEFT_46_SEQUENCE=ATCCAAGAAATAGCAGGGCTT
PRIMER_RIGHT_46_SEQUENCE=TTTCATTTGGAGGCACAACA
PRIMER_LEFT_46=777,21
PRIMER_RIGHT_46=1646,20
PRIMER_LEFT_46_TM=59.236
PRIMER_RIGHT_46_TM=60.088
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=870
PRIMER_PAIR_47_PENALTY=1.858702
PRIMER_LEFT_47_PENALTY=1.169021
PRIMER_RIGHT_47_PENALTY=0.689681
PRIMER_LEFT_47_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_47_SEQUENCE=AGGCACAACAAGGAGTGGAA
PRIMER_LEFT_47=821,21
PRIMER_RIGHT_47=1636,20
PRIMER_LEFT_47_TM=59.831
PRIMER_RIGHT_47_TM=60.690
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.5000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=816
PRIMER_PAIR_48_PENALTY=1.858702
PRIMER_LEFT_48_PENALTY=1.169021
PRIMER_RIGHT_48_PENALTY=0.689681
PRIMER_LEFT_48_SEQUENCE=TCGAATACAAGAGGCCAAAGA
PRIMER_RIGHT_48_SEQUENCE=TTGGAGGCACAACAAGGAGT
PRIMER_LEFT_48=821,21
PRIMER_RIGHT_48=1640,20
PRIMER_LEFT_48_TM=59.831
PRIMER_RIGHT_48_TM=60.690
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=820
PRIMER_PAIR_49_PENALTY=1.860894
PRIMER_LEFT_49_PENALTY=1.827949
PRIMER_RIGHT_49_PENALTY=0.032945
PRIMER_LEFT_49_SEQUENCE=GATCGAATACAAGAGGCCAAA
PRIMER_RIGHT_49_SEQUENCE=ACTCAATGCCACATTCACCA
PRIMER_LEFT_49=819,21
PRIMER_RIGHT_49=1561,20
PRIMER_LEFT_49_TM=59.172
PRIMER_RIGHT_49_TM=59.967
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.8000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=743
=
SEQUENCE_ID=MAGIC.149_2_3750249_3750265_oy-0_hi-0_101152_340539_TRUE
SEQUENCE_TARGET=950,116
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_2627870_2627920_ws-0_po-0_256271_495453_TRUE
SEQUENCE_TARGET=950,150
SEQUENCE_TEMPLATE=AATGCATCATTTTGATCTCCAAGCAAGTTTTCTCCTTACAAGAGATTAGAGAAAACCAAATTATCTCTGAGGCTTTGAAGAATCAAAACTGAATCAAGAGGANGTTTCCTGTTTAACAAAGATCACAAGAACAAATTTCNAAAAAGAAACTGTAACGAAGAACAACAAAGCAACAATCCTCGCAATATAACAAATAGTTCGTACAGAATCGTTCCTATTCNCCTTTCNATGGACAAAAGCGTTTNAAAAACGCTAATTTCTAGCAATTCGTTTTACCAGGTTANNNNNNNNNNNNNCAATTTCNGAAATTAACGATTTGATCTACNGTTCTACNCAATTTACCCCATCCAGATTAATGCTAGAANAAAANGAACGGGATCGCAGAACTTACCAGCACAGTAAAAATCAGAGCACGAAGAATGTTGGATCGGACGAACATGAGCAAAATTGGAGGCNAATGAAGAAGATTTTCAGGAGATATGATGAAAAAATTTGAAACCCNNAAAAAAAAGAAAAGGAAACCTNGTGCGACGACCGGATCCCTTCTCGCCTTAGGAGACTTTGGGATAAATCATGGAAGCCAAATTTTATCAAAGGAATATCCACTGACATTTATTTATTTTTACATTNAAAAAAANNNAAAAAAACTCACATNTAAANTATTTGTGATAAAGACCCTCAAGTTTGCTAATCTACCCAAAAGAGTCTGAATTATGTAAAATTATTATTGACGTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGATAATATTACATCGTTTACATATGAGTTCATCGTGAGTTCACGTCTGCTGACTTATTTTTGTAATTATTTATCTTCTTAACAAAAAAAACAGGTTTATCATTACATGGATATAGACTTATAGTCGACAATATTTGACACTCAAGCTGAAAGAACATGCTACAAAACATGTTGTGTGGACATTTATCNTATCTTAGTTGATATTATNAGANTTTTNAAGATGTTTGTTGGCTCTTGTGATTAAGAATCCAGTATACTGAACTAAGNACACAACATCAACAATGCTTATAATAAGAAAACAATAAAGATAAATACATNAAGCATACCACCAAATCGATCGATCAATAAATTCCATCTATTANAAGCTATAGCGATGGTTATACCTACGATATATGTGGCACCGATCCTATGTCCCAGACAACTTGATAAATGAGTTACNGTACTCTTTTGTATAATACTAGATTTTTGTGGTACAGAAAACTACACATCAACCTTGGCTCATGAACATGTATATGAATGAAAAGCAAGAGAGGCTACAATAAGAACATACCAAAACAAACACAGCGATGCGCATTAAGTGATTAAATAAACTTTAGAGATCTCTCCCAAAAGTATATACTTTAACTTTGTCCTTTGCTANTTGTTTGTTCATCTTTTATTTGGTGGGTTTTGATATGACATCGACTTCATTTCTCAGTTCNCAATCCATTTTATTTTAACTAAAAATGGGTNNAAGTCTTGCTTGAACAAGTTTGGACAAACTTCAACCAAACTANGTATAGTGTTTTATTGTATTTATGGTTTAACGTGTTGTTGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.389723
PRIMER_LEFT_0_PENALTY=0.277599
PRIMER_RIGHT_0_PENALTY=0.112124
PRIMER_LEFT_0_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_0_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_0=562,20
PRIMER_RIGHT_0=1286,20
PRIMER_LEFT_0_TM=59.722
PRIMER_RIGHT_0_TM=60.112
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.7000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=725
PRIMER_PAIR_1_PENALTY=0.418274
PRIMER_LEFT_1_PENALTY=0.306150
PRIMER_RIGHT_1_PENALTY=0.112124
PRIMER_LEFT_1_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_1_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_1=374,20
PRIMER_RIGHT_1=1286,20
PRIMER_LEFT_1_TM=59.694
PRIMER_RIGHT_1_TM=60.112
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=913
PRIMER_PAIR_2_PENALTY=0.426943
PRIMER_LEFT_2_PENALTY=0.277599
PRIMER_RIGHT_2_PENALTY=0.149344
PRIMER_LEFT_2_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_2_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_2=562,20
PRIMER_RIGHT_2=1352,20
PRIMER_LEFT_2_TM=59.722
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=791
PRIMER_PAIR_3_PENALTY=0.447803
PRIMER_LEFT_3_PENALTY=0.335679
PRIMER_RIGHT_3_PENALTY=0.112124
PRIMER_LEFT_3_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_3_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_3=432,20
PRIMER_RIGHT_3=1286,20
PRIMER_LEFT_3_TM=59.664
PRIMER_RIGHT_3_TM=60.112
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=855
PRIMER_PAIR_4_PENALTY=0.454113
PRIMER_LEFT_4_PENALTY=0.341988
PRIMER_RIGHT_4_PENALTY=0.112124
PRIMER_LEFT_4_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_4_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_4=379,20
PRIMER_RIGHT_4=1286,20
PRIMER_LEFT_4_TM=59.658
PRIMER_RIGHT_4_TM=60.112
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=908
PRIMER_PAIR_5_PENALTY=0.485022
PRIMER_LEFT_5_PENALTY=0.335679
PRIMER_RIGHT_5_PENALTY=0.149344
PRIMER_LEFT_5_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_5_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_5=432,20
PRIMER_RIGHT_5=1352,20
PRIMER_LEFT_5_TM=59.664
PRIMER_RIGHT_5_TM=60.149
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=921
PRIMER_PAIR_6_PENALTY=0.491332
PRIMER_LEFT_6_PENALTY=0.341988
PRIMER_RIGHT_6_PENALTY=0.149344
PRIMER_LEFT_6_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_6_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_6=379,20
PRIMER_RIGHT_6=1352,20
PRIMER_LEFT_6_TM=59.658
PRIMER_RIGHT_6_TM=60.149
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=974
PRIMER_PAIR_7_PENALTY=0.579537
PRIMER_LEFT_7_PENALTY=0.277599
PRIMER_RIGHT_7_PENALTY=0.301938
PRIMER_LEFT_7_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_7_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_7=562,20
PRIMER_RIGHT_7=1353,20
PRIMER_LEFT_7_TM=59.722
PRIMER_RIGHT_7_TM=60.302
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=792
PRIMER_PAIR_8_PENALTY=0.637616
PRIMER_LEFT_8_PENALTY=0.335679
PRIMER_RIGHT_8_PENALTY=0.301938
PRIMER_LEFT_8_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_8_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_8=432,20
PRIMER_RIGHT_8=1353,20
PRIMER_LEFT_8_TM=59.664
PRIMER_RIGHT_8_TM=60.302
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=922
PRIMER_PAIR_9_PENALTY=0.913753
PRIMER_LEFT_9_PENALTY=0.801629
PRIMER_RIGHT_9_PENALTY=0.112124
PRIMER_LEFT_9_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_9_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_9=371,20
PRIMER_RIGHT_9=1286,20
PRIMER_LEFT_9_TM=59.198
PRIMER_RIGHT_9_TM=60.112
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=916
PRIMER_PAIR_10_PENALTY=0.950973
PRIMER_LEFT_10_PENALTY=0.801629
PRIMER_RIGHT_10_PENALTY=0.149344
PRIMER_LEFT_10_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_10_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_10=371,20
PRIMER_RIGHT_10=1352,20
PRIMER_LEFT_10_TM=59.198
PRIMER_RIGHT_10_TM=60.149
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=5.7000
PRIMER_RIGHT_10_END_STABILITY=8.8000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=982
PRIMER_PAIR_11_PENALTY=1.084379
PRIMER_LEFT_11_PENALTY=0.972254
PRIMER_RIGHT_11_PENALTY=0.112124
PRIMER_LEFT_11_SEQUENCE=GATCGCAGAACTTACCAGCA
PRIMER_RIGHT_11_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_11=376,20
PRIMER_RIGHT_11=1286,20
PRIMER_LEFT_11_TM=59.028
PRIMER_RIGHT_11_TM=60.112
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=911
PRIMER_PAIR_12_PENALTY=1.159132
PRIMER_LEFT_12_PENALTY=0.277599
PRIMER_RIGHT_12_PENALTY=0.881533
PRIMER_LEFT_12_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_12_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_12=562,20
PRIMER_RIGHT_12=1201,20
PRIMER_LEFT_12_TM=59.722
PRIMER_RIGHT_12_TM=60.882
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=9.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=640
PRIMER_PAIR_13_PENALTY=1.159132
PRIMER_LEFT_13_PENALTY=0.277599
PRIMER_RIGHT_13_PENALTY=0.881533
PRIMER_LEFT_13_SEQUENCE=TGGGATAAATCATGGAAGCC
PRIMER_RIGHT_13_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_13=562,20
PRIMER_RIGHT_13=1203,20
PRIMER_LEFT_13_TM=59.722
PRIMER_RIGHT_13_TM=60.882
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=9.8000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=642
PRIMER_PAIR_14_PENALTY=1.187683
PRIMER_LEFT_14_PENALTY=0.306150
PRIMER_RIGHT_14_PENALTY=0.881533
PRIMER_LEFT_14_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_14_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_14=374,20
PRIMER_RIGHT_14=1201,20
PRIMER_LEFT_14_TM=59.694
PRIMER_RIGHT_14_TM=60.882
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=828
PRIMER_PAIR_15_PENALTY=1.187683
PRIMER_LEFT_15_PENALTY=0.306150
PRIMER_RIGHT_15_PENALTY=0.881533
PRIMER_LEFT_15_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_15_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_15=374,20
PRIMER_RIGHT_15=1203,20
PRIMER_LEFT_15_TM=59.694
PRIMER_RIGHT_15_TM=60.882
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=9.8000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=830
PRIMER_PAIR_16_PENALTY=1.215627
PRIMER_LEFT_16_PENALTY=1.103503
PRIMER_RIGHT_16_PENALTY=0.112124
PRIMER_LEFT_16_SEQUENCE=GATCGCAGAACTTACCAGCAC
PRIMER_RIGHT_16_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_16=376,21
PRIMER_RIGHT_16=1286,20
PRIMER_LEFT_16_TM=59.896
PRIMER_RIGHT_16_TM=60.112
PRIMER_LEFT_16_GC_PERCENT=52.381
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=911
PRIMER_PAIR_17_PENALTY=1.217212
PRIMER_LEFT_17_PENALTY=0.335679
PRIMER_RIGHT_17_PENALTY=0.881533
PRIMER_LEFT_17_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_17_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_17=432,20
PRIMER_RIGHT_17=1201,20
PRIMER_LEFT_17_TM=59.664
PRIMER_RIGHT_17_TM=60.882
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=770
PRIMER_PAIR_18_PENALTY=1.217212
PRIMER_LEFT_18_PENALTY=0.335679
PRIMER_RIGHT_18_PENALTY=0.881533
PRIMER_LEFT_18_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_18_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_18=432,20
PRIMER_RIGHT_18=1203,20
PRIMER_LEFT_18_TM=59.664
PRIMER_RIGHT_18_TM=60.882
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=9.8000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=772
PRIMER_PAIR_19_PENALTY=1.223522
PRIMER_LEFT_19_PENALTY=0.341988
PRIMER_RIGHT_19_PENALTY=0.881533
PRIMER_LEFT_19_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_19_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_19=379,20
PRIMER_RIGHT_19=1201,20
PRIMER_LEFT_19_TM=59.658
PRIMER_RIGHT_19_TM=60.882
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=823
PRIMER_PAIR_20_PENALTY=1.223522
PRIMER_LEFT_20_PENALTY=0.341988
PRIMER_RIGHT_20_PENALTY=0.881533
PRIMER_LEFT_20_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_20_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_20=379,20
PRIMER_RIGHT_20=1203,20
PRIMER_LEFT_20_TM=59.658
PRIMER_RIGHT_20_TM=60.882
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=9.8000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=825
PRIMER_PAIR_21_PENALTY=1.252847
PRIMER_LEFT_21_PENALTY=1.103503
PRIMER_RIGHT_21_PENALTY=0.149344
PRIMER_LEFT_21_SEQUENCE=GATCGCAGAACTTACCAGCAC
PRIMER_RIGHT_21_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_21=376,21
PRIMER_RIGHT_21=1352,20
PRIMER_LEFT_21_TM=59.896
PRIMER_RIGHT_21_TM=60.149
PRIMER_LEFT_21_GC_PERCENT=52.381
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=977
PRIMER_PAIR_22_PENALTY=1.477975
PRIMER_LEFT_22_PENALTY=0.596442
PRIMER_RIGHT_22_PENALTY=0.881533
PRIMER_LEFT_22_SEQUENCE=TCGCAGAACTTACCAGCACA
PRIMER_RIGHT_22_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_22=378,20
PRIMER_RIGHT_22=1201,20
PRIMER_LEFT_22_TM=60.596
PRIMER_RIGHT_22_TM=60.882
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=9.9000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=824
PRIMER_PAIR_23_PENALTY=1.477975
PRIMER_LEFT_23_PENALTY=0.596442
PRIMER_RIGHT_23_PENALTY=0.881533
PRIMER_LEFT_23_SEQUENCE=TCGCAGAACTTACCAGCACA
PRIMER_RIGHT_23_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_23=378,20
PRIMER_RIGHT_23=1203,20
PRIMER_LEFT_23_TM=60.596
PRIMER_RIGHT_23_TM=60.882
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=9.8000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=826
PRIMER_PAIR_24_PENALTY=1.513358
PRIMER_LEFT_24_PENALTY=1.401234
PRIMER_RIGHT_24_PENALTY=0.112124
PRIMER_LEFT_24_SEQUENCE=ACGGGATCGCAGAACTTACC
PRIMER_RIGHT_24_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_24=372,20
PRIMER_RIGHT_24=1286,20
PRIMER_LEFT_24_TM=61.401
PRIMER_RIGHT_24_TM=60.112
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.2000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=915
PRIMER_PAIR_25_PENALTY=1.548126
PRIMER_LEFT_25_PENALTY=1.436002
PRIMER_RIGHT_25_PENALTY=0.112124
PRIMER_LEFT_25_SEQUENCE=CACGAAGAATGTTGGATCGG
PRIMER_RIGHT_25_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_25=411,20
PRIMER_RIGHT_25=1286,20
PRIMER_LEFT_25_TM=61.436
PRIMER_RIGHT_25_TM=60.112
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.8000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=876
PRIMER_PAIR_26_PENALTY=1.550577
PRIMER_LEFT_26_PENALTY=1.401234
PRIMER_RIGHT_26_PENALTY=0.149344
PRIMER_LEFT_26_SEQUENCE=ACGGGATCGCAGAACTTACC
PRIMER_RIGHT_26_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_26=372,20
PRIMER_RIGHT_26=1352,20
PRIMER_LEFT_26_TM=61.401
PRIMER_RIGHT_26_TM=60.149
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.2000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=981
PRIMER_PAIR_27_PENALTY=1.552062
PRIMER_LEFT_27_PENALTY=1.439937
PRIMER_RIGHT_27_PENALTY=0.112124
PRIMER_LEFT_27_SEQUENCE=GAACATGAGCAAAATTGGAGG
PRIMER_RIGHT_27_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_27=433,21
PRIMER_RIGHT_27=1286,20
PRIMER_LEFT_27_TM=59.560
PRIMER_RIGHT_27_TM=60.112
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=854
PRIMER_PAIR_28_PENALTY=1.585346
PRIMER_LEFT_28_PENALTY=1.436002
PRIMER_RIGHT_28_PENALTY=0.149344
PRIMER_LEFT_28_SEQUENCE=CACGAAGAATGTTGGATCGG
PRIMER_RIGHT_28_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_28=411,20
PRIMER_RIGHT_28=1352,20
PRIMER_LEFT_28_TM=61.436
PRIMER_RIGHT_28_TM=60.149
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.8000
PRIMER_RIGHT_28_END_STABILITY=8.8000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=942
PRIMER_PAIR_29_PENALTY=1.589281
PRIMER_LEFT_29_PENALTY=1.439937
PRIMER_RIGHT_29_PENALTY=0.149344
PRIMER_LEFT_29_SEQUENCE=GAACATGAGCAAAATTGGAGG
PRIMER_RIGHT_29_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_29=433,21
PRIMER_RIGHT_29=1352,20
PRIMER_LEFT_29_TM=59.560
PRIMER_RIGHT_29_TM=60.149
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=920
PRIMER_PAIR_30_PENALTY=1.669338
PRIMER_LEFT_30_PENALTY=1.557214
PRIMER_RIGHT_30_PENALTY=0.112124
PRIMER_LEFT_30_SEQUENCE=AGAGCACGAAGAATGTTGGA
PRIMER_RIGHT_30_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_30=407,20
PRIMER_RIGHT_30=1286,20
PRIMER_LEFT_30_TM=58.443
PRIMER_RIGHT_30_TM=60.112
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=880
PRIMER_PAIR_31_PENALTY=1.683162
PRIMER_LEFT_31_PENALTY=0.801629
PRIMER_RIGHT_31_PENALTY=0.881533
PRIMER_LEFT_31_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_31_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_31=371,20
PRIMER_RIGHT_31=1201,20
PRIMER_LEFT_31_TM=59.198
PRIMER_RIGHT_31_TM=60.882
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.7000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=831
PRIMER_PAIR_32_PENALTY=1.683162
PRIMER_LEFT_32_PENALTY=0.801629
PRIMER_RIGHT_32_PENALTY=0.881533
PRIMER_LEFT_32_SEQUENCE=AACGGGATCGCAGAACTTAC
PRIMER_RIGHT_32_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_32=371,20
PRIMER_RIGHT_32=1203,20
PRIMER_LEFT_32_TM=59.198
PRIMER_RIGHT_32_TM=60.882
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=5.7000
PRIMER_RIGHT_32_END_STABILITY=9.8000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=833
PRIMER_PAIR_33_PENALTY=1.700056
PRIMER_LEFT_33_PENALTY=1.587932
PRIMER_RIGHT_33_PENALTY=0.112124
PRIMER_LEFT_33_SEQUENCE=ATCGGACGAACATGAGCAAA
PRIMER_RIGHT_33_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_33=426,20
PRIMER_RIGHT_33=1286,20
PRIMER_LEFT_33_TM=61.588
PRIMER_RIGHT_33_TM=60.112
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.8000
PRIMER_RIGHT_33_END_STABILITY=6.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=861
PRIMER_PAIR_34_PENALTY=1.703171
PRIMER_LEFT_34_PENALTY=1.401234
PRIMER_RIGHT_34_PENALTY=0.301938
PRIMER_LEFT_34_SEQUENCE=ACGGGATCGCAGAACTTACC
PRIMER_RIGHT_34_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_34=372,20
PRIMER_RIGHT_34=1353,20
PRIMER_LEFT_34_TM=61.401
PRIMER_RIGHT_34_TM=60.302
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.2000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=982
PRIMER_PAIR_35_PENALTY=1.706557
PRIMER_LEFT_35_PENALTY=1.557214
PRIMER_RIGHT_35_PENALTY=0.149344
PRIMER_LEFT_35_SEQUENCE=AGAGCACGAAGAATGTTGGA
PRIMER_RIGHT_35_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_35=407,20
PRIMER_RIGHT_35=1352,20
PRIMER_LEFT_35_TM=58.443
PRIMER_RIGHT_35_TM=60.149
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=8.8000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=946
PRIMER_PAIR_36_PENALTY=1.723117
PRIMER_LEFT_36_PENALTY=0.306150
PRIMER_RIGHT_36_PENALTY=1.416967
PRIMER_LEFT_36_SEQUENCE=GGGATCGCAGAACTTACCAG
PRIMER_RIGHT_36_SEQUENCE=GCCTCTCTTGCTTTTCATTCA
PRIMER_LEFT_36=374,20
PRIMER_RIGHT_36=1317,21
PRIMER_LEFT_36_TM=59.694
PRIMER_RIGHT_36_TM=59.583
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=944
PRIMER_PAIR_37_PENALTY=1.732426
PRIMER_LEFT_37_PENALTY=1.620302
PRIMER_RIGHT_37_PENALTY=0.112124
PRIMER_LEFT_37_SEQUENCE=CGCCTTAGGAGACTTTGGGA
PRIMER_RIGHT_37_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_37=547,20
PRIMER_RIGHT_37=1286,20
PRIMER_LEFT_37_TM=61.620
PRIMER_RIGHT_37_TM=60.112
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=6.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=740
PRIMER_PAIR_38_PENALTY=1.734786
PRIMER_LEFT_38_PENALTY=1.622662
PRIMER_RIGHT_38_PENALTY=0.112124
PRIMER_LEFT_38_SEQUENCE=CGAACATGAGCAAAATTGGAG
PRIMER_RIGHT_38_SEQUENCE=CATGAGCCAAGGTTGATGTG
PRIMER_LEFT_38=432,21
PRIMER_RIGHT_38=1286,20
PRIMER_LEFT_38_TM=60.623
PRIMER_RIGHT_38_TM=60.112
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=855
PRIMER_PAIR_39_PENALTY=1.737276
PRIMER_LEFT_39_PENALTY=1.587932
PRIMER_RIGHT_39_PENALTY=0.149344
PRIMER_LEFT_39_SEQUENCE=ATCGGACGAACATGAGCAAA
PRIMER_RIGHT_39_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_39=426,20
PRIMER_RIGHT_39=1352,20
PRIMER_LEFT_39_TM=61.588
PRIMER_RIGHT_39_TM=60.149
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.8000
PRIMER_RIGHT_39_END_STABILITY=8.8000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=927
PRIMER_PAIR_40_PENALTY=1.737939
PRIMER_LEFT_40_PENALTY=1.436002
PRIMER_RIGHT_40_PENALTY=0.301938
PRIMER_LEFT_40_SEQUENCE=CACGAAGAATGTTGGATCGG
PRIMER_RIGHT_40_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_40=411,20
PRIMER_RIGHT_40=1353,20
PRIMER_LEFT_40_TM=61.436
PRIMER_RIGHT_40_TM=60.302
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.8000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=943
PRIMER_PAIR_41_PENALTY=1.741875
PRIMER_LEFT_41_PENALTY=1.439937
PRIMER_RIGHT_41_PENALTY=0.301938
PRIMER_LEFT_41_SEQUENCE=GAACATGAGCAAAATTGGAGG
PRIMER_RIGHT_41_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_41=433,21
PRIMER_RIGHT_41=1353,20
PRIMER_LEFT_41_TM=59.560
PRIMER_RIGHT_41_TM=60.302
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.4000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=921
PRIMER_PAIR_42_PENALTY=1.752646
PRIMER_LEFT_42_PENALTY=0.335679
PRIMER_RIGHT_42_PENALTY=1.416967
PRIMER_LEFT_42_SEQUENCE=CGAACATGAGCAAAATTGGA
PRIMER_RIGHT_42_SEQUENCE=GCCTCTCTTGCTTTTCATTCA
PRIMER_LEFT_42=432,20
PRIMER_RIGHT_42=1317,21
PRIMER_LEFT_42_TM=59.664
PRIMER_RIGHT_42_TM=59.583
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=886
PRIMER_PAIR_43_PENALTY=1.758955
PRIMER_LEFT_43_PENALTY=0.341988
PRIMER_RIGHT_43_PENALTY=1.416967
PRIMER_LEFT_43_SEQUENCE=CGCAGAACTTACCAGCACAG
PRIMER_RIGHT_43_SEQUENCE=GCCTCTCTTGCTTTTCATTCA
PRIMER_LEFT_43=379,20
PRIMER_RIGHT_43=1317,21
PRIMER_LEFT_43_TM=59.658
PRIMER_RIGHT_43_TM=59.583
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=939
PRIMER_PAIR_44_PENALTY=1.769645
PRIMER_LEFT_44_PENALTY=1.620302
PRIMER_RIGHT_44_PENALTY=0.149344
PRIMER_LEFT_44_SEQUENCE=CGCCTTAGGAGACTTTGGGA
PRIMER_RIGHT_44_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_44=547,20
PRIMER_RIGHT_44=1352,20
PRIMER_LEFT_44_TM=61.620
PRIMER_RIGHT_44_TM=60.149
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.7000
PRIMER_RIGHT_44_END_STABILITY=8.8000
PRIMER_PAIR_44_COMPL_ANY=2.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=806
PRIMER_PAIR_45_PENALTY=1.772006
PRIMER_LEFT_45_PENALTY=1.622662
PRIMER_RIGHT_45_PENALTY=0.149344
PRIMER_LEFT_45_SEQUENCE=CGAACATGAGCAAAATTGGAG
PRIMER_RIGHT_45_SEQUENCE=CATCGCTGTGTTTGTTTTGG
PRIMER_LEFT_45=432,21
PRIMER_RIGHT_45=1352,20
PRIMER_LEFT_45_TM=60.623
PRIMER_RIGHT_45_TM=60.149
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.8000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=921
PRIMER_PAIR_46_PENALTY=1.853788
PRIMER_LEFT_46_PENALTY=0.972254
PRIMER_RIGHT_46_PENALTY=0.881533
PRIMER_LEFT_46_SEQUENCE=GATCGCAGAACTTACCAGCA
PRIMER_RIGHT_46_SEQUENCE=TCTGGGACATAGGATCGGTG
PRIMER_LEFT_46=376,20
PRIMER_RIGHT_46=1201,20
PRIMER_LEFT_46_TM=59.028
PRIMER_RIGHT_46_TM=60.882
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=9.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=826
PRIMER_PAIR_47_PENALTY=1.853788
PRIMER_LEFT_47_PENALTY=0.972254
PRIMER_RIGHT_47_PENALTY=0.881533
PRIMER_LEFT_47_SEQUENCE=GATCGCAGAACTTACCAGCA
PRIMER_RIGHT_47_SEQUENCE=TGTCTGGGACATAGGATCGG
PRIMER_LEFT_47=376,20
PRIMER_RIGHT_47=1203,20
PRIMER_LEFT_47_TM=59.028
PRIMER_RIGHT_47_TM=60.882
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=9.8000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=828
PRIMER_PAIR_48_PENALTY=1.859151
PRIMER_LEFT_48_PENALTY=1.557214
PRIMER_RIGHT_48_PENALTY=0.301938
PRIMER_LEFT_48_SEQUENCE=AGAGCACGAAGAATGTTGGA
PRIMER_RIGHT_48_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_48=407,20
PRIMER_RIGHT_48=1353,20
PRIMER_LEFT_48_TM=58.443
PRIMER_RIGHT_48_TM=60.302
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=947
PRIMER_PAIR_49_PENALTY=1.922239
PRIMER_LEFT_49_PENALTY=1.620302
PRIMER_RIGHT_49_PENALTY=0.301938
PRIMER_LEFT_49_SEQUENCE=CGCCTTAGGAGACTTTGGGA
PRIMER_RIGHT_49_SEQUENCE=GCATCGCTGTGTTTGTTTTG
PRIMER_LEFT_49=547,20
PRIMER_RIGHT_49=1353,20
PRIMER_LEFT_49_TM=61.620
PRIMER_RIGHT_49_TM=60.302
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=7.6000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=807
=
SEQUENCE_ID=MAGIC.105_3_14562472_14562501_no-0_rsch-4_10272_70230_TRUE
SEQUENCE_TARGET=950,129
SEQUENCE_TEMPLATE=TCAGNACTAGTTTATTAGTGGTGAAGCCAGAATAATCGACACTTTCAATTTTGTTGATCATGANGGTAANTATAGGACGAGTCCTTTCNGTTTCAAAATAGTCTTCTATNGAATTACTACGGTNANACNCTGTGACGATTTTNCATNTCAAGTTCCCGAGAAATANTTTAATTAAGGAGTTTNCTNAAANTTTGTCTGGGAAATTAGANAGNCAGATATTGTTTGGNTTGTATTAATATTTTNATTGGTATNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTAGTCAACATGGAGTACTATGAAGTAGTGGAGCAAATAAAGGAGNANAAAAATNNTATGTTAANTAATNCAAATCATAGAGATATTACGTATTAGGGAAGTAGATTNTGCATATTAATTAGGGAAGTAGATNTCCNTTGTNATATTTTCTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNGATTGGTNACNTATAAANGAATANATGGANNNNNNNNNAACATNACNAAAANGAACAAACANGAAAAGNNNNNNNNNNNAAAATAGGAATNANGAGGAATAGGNGCNNAGNAAAAAAANAACAAATGTTACTGTAAATNATAANCAAAACATAGAACNAATAGTAACAAAAATNAAAAANTAAATAANTTANTTTTTANATAANNTATTAAACNAAATNGCCAAGTTATCATAGACTCAAACAATTTCATNGCNAAGTTATCANGTAACTTGNNTGGCTTGGTTTTCTTTCTTANTNTAAGTTCACAAAATCAGAGAGTTAATTGTNGAGTACTTGATTTGCGTTTGAAAATATGAGATCAACTATTTATAAAAGCAAATGATGTAATTGAGAACTNATAACCTTGGCATGTCTTTGGNTTNGTCACNATGGTATAAGANTTTTCTTNTTCTGNAANTNTTTCACAGGGAACCCTNAAAATGAAATCCATGTCTTNNTACAGTCAGTTTCTCAAAAATATGTGATTAGATGAGAATTGTAATAATTCACAAAGTATAAAGCAAANTAATGTTNAAACTGAAACCAGAATAATAATTGCAAAACGAAGAGACATATGAATCTCNAGAATATGAACAAATTGATACCTTAATTNCAATTNCACTTGTTTTGTTCTTCAGCCATAATTGNCATGGAANGGGAAAGGAGAGTGNAAATGAATTATGGTNCGAGAGATGAAAGTGGACAAAAATACTGTAAAATNGTAAATCTGNCATGNCGTGGANATGGAGGAAGANAAAGTAATTGATCNNAAAGCGAGAANTTTGGGAATCGTAATTAGAAAATCCGCNAAGAAAATTNAACAATATAATTAGCAAGAACNGTTGCAAGAGAAGATCGTGAGGAAGATGCCGAGAAAGAAGNAAANATTTTGGGATTAATTTTCNGTNAANTTTNTNNTAATGAGTAANTANTTATTGTTTTANGNTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTGTTNNGTTCC
PRIMER_LEFT_NUM_RETURNED=32
PRIMER_RIGHT_NUM_RETURNED=32
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=32
PRIMER_PAIR_0_PENALTY=8.619823
PRIMER_LEFT_0_PENALTY=5.181553
PRIMER_RIGHT_0_PENALTY=3.438269
PRIMER_LEFT_0_SEQUENCE=TGAAGTAGTGGAGCAAATAAAGGAG
PRIMER_RIGHT_0_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_0=735,25
PRIMER_RIGHT_0=1618,20
PRIMER_LEFT_0_TM=60.182
PRIMER_RIGHT_0_TM=56.562
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=884
PRIMER_PAIR_1_PENALTY=9.039698
PRIMER_LEFT_1_PENALTY=5.601428
PRIMER_RIGHT_1_PENALTY=3.438269
PRIMER_LEFT_1_SEQUENCE=TGGAGTACTATGAAGTAGTGGAGCA
PRIMER_RIGHT_1_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_1=725,25
PRIMER_RIGHT_1=1618,20
PRIMER_LEFT_1_TM=59.399
PRIMER_RIGHT_1_TM=56.562
PRIMER_LEFT_1_GC_PERCENT=44.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=894
PRIMER_PAIR_2_PENALTY=9.654204
PRIMER_LEFT_2_PENALTY=6.215934
PRIMER_RIGHT_2_PENALTY=3.438269
PRIMER_LEFT_2_SEQUENCE=GAAGTAGTGGAGCAAATAAAGGAG
PRIMER_RIGHT_2_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_2=736,24
PRIMER_RIGHT_2=1618,20
PRIMER_LEFT_2_TM=57.784
PRIMER_RIGHT_2_TM=56.562
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=883
PRIMER_PAIR_3_PENALTY=9.775603
PRIMER_LEFT_3_PENALTY=6.337334
PRIMER_RIGHT_3_PENALTY=3.438269
PRIMER_LEFT_3_SEQUENCE=ATGGAGTACTATGAAGTAGTGGAGCA
PRIMER_RIGHT_3_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_3=724,26
PRIMER_RIGHT_3=1618,20
PRIMER_LEFT_3_TM=59.663
PRIMER_RIGHT_3_TM=56.562
PRIMER_LEFT_3_GC_PERCENT=42.308
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=895
PRIMER_PAIR_4_PENALTY=10.003415
PRIMER_LEFT_4_PENALTY=6.565146
PRIMER_RIGHT_4_PENALTY=3.438269
PRIMER_LEFT_4_SEQUENCE=TGGAGTACTATGAAGTAGTGGAGCAA
PRIMER_RIGHT_4_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_4=725,26
PRIMER_RIGHT_4=1618,20
PRIMER_LEFT_4_TM=60.565
PRIMER_RIGHT_4_TM=56.562
PRIMER_LEFT_4_GC_PERCENT=42.308
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=894
PRIMER_PAIR_5_PENALTY=10.097723
PRIMER_LEFT_5_PENALTY=6.659453
PRIMER_RIGHT_5_PENALTY=3.438269
PRIMER_LEFT_5_SEQUENCE=GGAGTACTATGAAGTAGTGGAGCAAA
PRIMER_RIGHT_5_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_5=726,26
PRIMER_RIGHT_5=1618,20
PRIMER_LEFT_5_TM=59.341
PRIMER_RIGHT_5_TM=56.562
PRIMER_LEFT_5_GC_PERCENT=42.308
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.8000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=893
PRIMER_PAIR_6_PENALTY=10.297058
PRIMER_LEFT_6_PENALTY=6.858788
PRIMER_RIGHT_6_PENALTY=3.438269
PRIMER_LEFT_6_SEQUENCE=GGAGTACTATGAAGTAGTGGAGCAA
PRIMER_RIGHT_6_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_6=726,25
PRIMER_RIGHT_6=1618,20
PRIMER_LEFT_6_TM=58.141
PRIMER_RIGHT_6_TM=56.562
PRIMER_LEFT_6_GC_PERCENT=44.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=893
PRIMER_PAIR_7_PENALTY=10.407351
PRIMER_LEFT_7_PENALTY=6.969081
PRIMER_RIGHT_7_PENALTY=3.438269
PRIMER_LEFT_7_SEQUENCE=GAAGTAGTGGAGCAAATAAAGG
PRIMER_RIGHT_7_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_7=736,22
PRIMER_RIGHT_7=1618,20
PRIMER_LEFT_7_TM=55.031
PRIMER_RIGHT_7_TM=56.562
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=883
PRIMER_PAIR_8_PENALTY=10.534973
PRIMER_LEFT_8_PENALTY=7.096703
PRIMER_RIGHT_8_PENALTY=3.438269
PRIMER_LEFT_8_SEQUENCE=TCAACATGGAGTACTATGAAGTAGTGG
PRIMER_RIGHT_8_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_8=719,27
PRIMER_RIGHT_8=1618,20
PRIMER_LEFT_8_TM=59.903
PRIMER_RIGHT_8_TM=56.562
PRIMER_LEFT_8_GC_PERCENT=40.741
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=900
PRIMER_PAIR_9_PENALTY=10.616864
PRIMER_LEFT_9_PENALTY=7.178594
PRIMER_RIGHT_9_PENALTY=3.438269
PRIMER_LEFT_9_SEQUENCE=GGAGTACTATGAAGTAGTGGAGCA
PRIMER_RIGHT_9_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_9=726,24
PRIMER_RIGHT_9=1618,20
PRIMER_LEFT_9_TM=56.821
PRIMER_RIGHT_9_TM=56.562
PRIMER_LEFT_9_GC_PERCENT=45.833
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=893
PRIMER_PAIR_10_PENALTY=11.134276
PRIMER_LEFT_10_PENALTY=7.696006
PRIMER_RIGHT_10_PENALTY=3.438269
PRIMER_LEFT_10_SEQUENCE=CAACATGGAGTACTATGAAGTAGTGG
PRIMER_RIGHT_10_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_10=720,26
PRIMER_RIGHT_10=1618,20
PRIMER_LEFT_10_TM=58.304
PRIMER_RIGHT_10_TM=56.562
PRIMER_LEFT_10_GC_PERCENT=42.308
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=899
PRIMER_PAIR_11_PENALTY=11.203983
PRIMER_LEFT_11_PENALTY=7.765713
PRIMER_RIGHT_11_PENALTY=3.438269
PRIMER_LEFT_11_SEQUENCE=ATGGAGTACTATGAAGTAGTGGAGCAA
PRIMER_RIGHT_11_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_11=724,27
PRIMER_RIGHT_11=1618,20
PRIMER_LEFT_11_TM=60.766
PRIMER_RIGHT_11_TM=56.562
PRIMER_LEFT_11_GC_PERCENT=40.741
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=895
PRIMER_PAIR_12_PENALTY=12.018337
PRIMER_LEFT_12_PENALTY=8.580067
PRIMER_RIGHT_12_PENALTY=3.438269
PRIMER_LEFT_12_SEQUENCE=GTCAACATGGAGTACTATGAAGTAGTGG
PRIMER_RIGHT_12_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_12=718,28
PRIMER_RIGHT_12=1618,20
PRIMER_LEFT_12_TM=60.580
PRIMER_RIGHT_12_TM=56.562
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=901
PRIMER_PAIR_13_PENALTY=12.049806
PRIMER_LEFT_13_PENALTY=8.611536
PRIMER_RIGHT_13_PENALTY=3.438269
PRIMER_LEFT_13_SEQUENCE=AACATGGAGTACTATGAAGTAGTGGAG
PRIMER_RIGHT_13_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_13=721,27
PRIMER_RIGHT_13=1618,20
PRIMER_LEFT_13_TM=58.388
PRIMER_RIGHT_13_TM=56.562
PRIMER_LEFT_13_GC_PERCENT=40.741
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=898
PRIMER_PAIR_14_PENALTY=12.066055
PRIMER_LEFT_14_PENALTY=8.627786
PRIMER_RIGHT_14_PENALTY=3.438269
PRIMER_LEFT_14_SEQUENCE=TGGAGTACTATGAAGTAGTGGAGCAAA
PRIMER_RIGHT_14_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_14=725,27
PRIMER_RIGHT_14=1618,20
PRIMER_LEFT_14_TM=61.628
PRIMER_RIGHT_14_TM=56.562
PRIMER_LEFT_14_GC_PERCENT=40.741
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.8000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=894
PRIMER_PAIR_15_PENALTY=12.122282
PRIMER_LEFT_15_PENALTY=8.684013
PRIMER_RIGHT_15_PENALTY=3.438269
PRIMER_LEFT_15_SEQUENCE=CAACATGGAGTACTATGAAGTAGTGGAG
PRIMER_RIGHT_15_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_15=720,28
PRIMER_RIGHT_15=1618,20
PRIMER_LEFT_15_TM=60.684
PRIMER_RIGHT_15_TM=56.562
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=899
PRIMER_PAIR_16_PENALTY=12.130928
PRIMER_LEFT_16_PENALTY=8.692658
PRIMER_RIGHT_16_PENALTY=3.438269
PRIMER_LEFT_16_SEQUENCE=CATGGAGTACTATGAAGTAGTGGAG
PRIMER_RIGHT_16_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_16=723,25
PRIMER_RIGHT_16=1618,20
PRIMER_LEFT_16_TM=56.307
PRIMER_RIGHT_16_TM=56.562
PRIMER_LEFT_16_GC_PERCENT=44.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=896
PRIMER_PAIR_17_PENALTY=12.264957
PRIMER_LEFT_17_PENALTY=8.826687
PRIMER_RIGHT_17_PENALTY=3.438269
PRIMER_LEFT_17_SEQUENCE=GAGTACTATGAAGTAGTGGAGCAAA
PRIMER_RIGHT_17_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_17=727,25
PRIMER_RIGHT_17=1618,20
PRIMER_LEFT_17_TM=56.173
PRIMER_RIGHT_17_TM=56.562
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.8000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=892
PRIMER_PAIR_18_PENALTY=12.286294
PRIMER_LEFT_18_PENALTY=8.848025
PRIMER_RIGHT_18_PENALTY=3.438269
PRIMER_LEFT_18_SEQUENCE=ACATGGAGTACTATGAAGTAGTGGAG
PRIMER_RIGHT_18_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_18=722,26
PRIMER_RIGHT_18=1618,20
PRIMER_LEFT_18_TM=57.152
PRIMER_RIGHT_18_TM=56.562
PRIMER_LEFT_18_GC_PERCENT=42.308
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=897
PRIMER_PAIR_19_PENALTY=12.428856
PRIMER_LEFT_19_PENALTY=8.990587
PRIMER_RIGHT_19_PENALTY=3.438269
PRIMER_LEFT_19_SEQUENCE=CATGGAGTACTATGAAGTAGTGGAGCA
PRIMER_RIGHT_19_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_19=723,27
PRIMER_RIGHT_19=1618,20
PRIMER_LEFT_19_TM=61.991
PRIMER_RIGHT_19_TM=56.562
PRIMER_LEFT_19_GC_PERCENT=44.444
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=896
PRIMER_PAIR_20_PENALTY=12.650933
PRIMER_LEFT_20_PENALTY=9.212664
PRIMER_RIGHT_20_PENALTY=3.438269
PRIMER_LEFT_20_SEQUENCE=AACATGGAGTACTATGAAGTAGTGG
PRIMER_RIGHT_20_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_20=721,25
PRIMER_RIGHT_20=1618,20
PRIMER_LEFT_20_TM=55.787
PRIMER_RIGHT_20_TM=56.562
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=898
PRIMER_PAIR_21_PENALTY=13.749749
PRIMER_LEFT_21_PENALTY=10.311480
PRIMER_RIGHT_21_PENALTY=3.438269
PRIMER_LEFT_21_SEQUENCE=AGTCAACATGGAGTACTATGAAGTAGTGG
PRIMER_RIGHT_21_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_21=717,29
PRIMER_RIGHT_21=1618,20
PRIMER_LEFT_21_TM=61.311
PRIMER_RIGHT_21_TM=56.562
PRIMER_LEFT_21_GC_PERCENT=41.379
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=902
PRIMER_PAIR_22_PENALTY=14.031651
PRIMER_LEFT_22_PENALTY=10.593382
PRIMER_RIGHT_22_PENALTY=3.438269
PRIMER_LEFT_22_SEQUENCE=ACATGGAGTACTATGAAGTAGTGGAGCA
PRIMER_RIGHT_22_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_22=722,28
PRIMER_RIGHT_22=1618,20
PRIMER_LEFT_22_TM=62.593
PRIMER_RIGHT_22_TM=56.562
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=897
PRIMER_PAIR_23_PENALTY=14.404810
PRIMER_LEFT_23_PENALTY=10.966540
PRIMER_RIGHT_23_PENALTY=3.438269
PRIMER_LEFT_23_SEQUENCE=CATGGAGTACTATGAAGTAGTGGAGCAA
PRIMER_RIGHT_23_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_23=723,28
PRIMER_RIGHT_23=1618,20
PRIMER_LEFT_23_TM=62.967
PRIMER_RIGHT_23_TM=56.562
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=896
PRIMER_PAIR_24_PENALTY=14.565039
PRIMER_LEFT_24_PENALTY=11.126769
PRIMER_RIGHT_24_PENALTY=3.438269
PRIMER_LEFT_24_SEQUENCE=TCAACATGGAGTACTATGAAGTAGTGGAG
PRIMER_RIGHT_24_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_24=719,29
PRIMER_RIGHT_24=1618,20
PRIMER_LEFT_24_TM=62.127
PRIMER_RIGHT_24_TM=56.562
PRIMER_LEFT_24_GC_PERCENT=41.379
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=900
PRIMER_PAIR_25_PENALTY=14.719577
PRIMER_LEFT_25_PENALTY=11.281308
PRIMER_RIGHT_25_PENALTY=3.438269
PRIMER_LEFT_25_SEQUENCE=TAGTCAACATGGAGTACTATGAAGTAGTGG
PRIMER_RIGHT_25_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_25=716,30
PRIMER_RIGHT_25=1618,20
PRIMER_LEFT_25_TM=61.281
PRIMER_RIGHT_25_TM=56.562
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=903
PRIMER_PAIR_26_PENALTY=15.952493
PRIMER_LEFT_26_PENALTY=12.514223
PRIMER_RIGHT_26_PENALTY=3.438269
PRIMER_LEFT_26_SEQUENCE=ACATGGAGTACTATGAAGTAGTGGAGCAA
PRIMER_RIGHT_26_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_26=722,29
PRIMER_RIGHT_26=1618,20
PRIMER_LEFT_26_TM=63.514
PRIMER_RIGHT_26_TM=56.562
PRIMER_LEFT_26_GC_PERCENT=41.379
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=897
PRIMER_PAIR_27_PENALTY=15.952493
PRIMER_LEFT_27_PENALTY=12.514223
PRIMER_RIGHT_27_PENALTY=3.438269
PRIMER_LEFT_27_SEQUENCE=AACATGGAGTACTATGAAGTAGTGGAGCA
PRIMER_RIGHT_27_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_27=721,29
PRIMER_RIGHT_27=1618,20
PRIMER_LEFT_27_TM=63.514
PRIMER_RIGHT_27_TM=56.562
PRIMER_LEFT_27_GC_PERCENT=41.379
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=898
PRIMER_PAIR_28_PENALTY=16.134159
PRIMER_LEFT_28_PENALTY=12.695889
PRIMER_RIGHT_28_PENALTY=3.438269
PRIMER_LEFT_28_SEQUENCE=GTCAACATGGAGTACTATGAAGTAGTGGAG
PRIMER_RIGHT_28_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_28=718,30
PRIMER_RIGHT_28=1618,20
PRIMER_LEFT_28_TM=62.696
PRIMER_RIGHT_28_TM=56.562
PRIMER_LEFT_28_GC_PERCENT=43.333
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=901
PRIMER_PAIR_29_PENALTY=16.299372
PRIMER_LEFT_29_PENALTY=12.861102
PRIMER_RIGHT_29_PENALTY=3.438269
PRIMER_LEFT_29_SEQUENCE=CATGGAGTACTATGAAGTAGTGGAGCAAA
PRIMER_RIGHT_29_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_29=723,29
PRIMER_RIGHT_29=1618,20
PRIMER_LEFT_29_TM=63.861
PRIMER_RIGHT_29_TM=56.562
PRIMER_LEFT_29_GC_PERCENT=41.379
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.8000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=896
PRIMER_PAIR_30_PENALTY=17.798779
PRIMER_LEFT_30_PENALTY=14.360510
PRIMER_RIGHT_30_PENALTY=3.438269
PRIMER_LEFT_30_SEQUENCE=ACATGGAGTACTATGAAGTAGTGGAGCAAA
PRIMER_RIGHT_30_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_30=722,30
PRIMER_RIGHT_30=1618,20
PRIMER_LEFT_30_TM=64.361
PRIMER_RIGHT_30_TM=56.562
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.8000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=897
PRIMER_PAIR_31_PENALTY=17.798779
PRIMER_LEFT_31_PENALTY=14.360510
PRIMER_RIGHT_31_PENALTY=3.438269
PRIMER_LEFT_31_SEQUENCE=AACATGGAGTACTATGAAGTAGTGGAGCAA
PRIMER_RIGHT_31_SEQUENCE=TGGCTGAAGAACAAAACAAG
PRIMER_LEFT_31=721,30
PRIMER_RIGHT_31=1618,20
PRIMER_LEFT_31_TM=64.361
PRIMER_RIGHT_31_TM=56.562
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=898
=
SEQUENCE_ID=MAGIC.338_1_12847129_12847183_ler-0_can-0_521_155400_TRUE
SEQUENCE_TARGET=950,154
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNGTGNCTATAGNCAATAAAAAANCATTTGTTTCATGTTCCNAAAAATANAAANANANTTTTAGGNCCCCCNAATCTTGTAAATATAGTGTATNAGAGTGGTTTTAGANGACTCTTTTATTNCGTGAGCACCCAAGTTCGANTCTTTTGAACAANNNNNTTTTACNTATATACACAATTAGGNTTTANAAANAGAAAATTGAGAATGGATTNAGAGAGATAGTTTATTGAGACANAAGGANAGANAATTGTATCTTATTAGTTANTTNTTACTNTTTTGTNTTNAANANTTTATATGNTTTNAAAAANAAAATNGATTTATTTTATTTGAAAAGNTCTATNTATTTANTTTTTCTTCTTGATCCCAANTTTANCTTTGCCTTAANCNTNGGCACGTNTTGCAGCGNGATAANTTATGAAGAACCGAGACANTGCTTTTAAGGANAGAATACGAAANTAATNTTGAGTNTNAATNGATCTCAAGNANGACANTCGTAGCTCNNAATTNTANTNNNATNNNNCTTCAAAGACTTCGTTTGANAATACTNTTCTNAGTGCNTATCAAAANCTCTTNAATATTATTAATCAAATCAACAACNNAATAATACANTNGGCCTAAGANGGCTATATATAATAAAAGCTCAAAACCCTAAANATAGAATATTCTAGAATAANTAANTAAAAGATAANAATANTAGATNTTTGNNNATNTTCCAAATATCTNGCTGCATCANCACGACACNGGNAGTAGAGCACAACANTTNGACAATANGCTATNATTATAAACNANNCNAACAACACNTTGAATAATAGAGAACACAAACTACAGTTAGATTNCAAAGTACNNNNNNNNNNNNNNNNNNNNNNNNGTTAATTTTTACGTTTATTANAAAGTGGGTTAAGTTGAAGTTTGGCCACAAAACAAATNAAAAGNCCAACACANTAGACTNCATCTTCTCCAATCTACACAAGNACGATTCNGATCAACATCACCNGNCATAGACAATGGCGACTAAGGNCATCTCCAAAGNCTACANTTTCGCNTCTACNTGGAAANAGGTAATCTNANCTCGGCTTTGATCNATTGAATGATGCTCGGTTTTATTGAAATATANTGAATTGTAATTAGCGTGTTGCAGAGAGCTNCTTTGACAGAGAAGCAACAAGCANNTATTGTTTCTTTATNCCACGNANTTGNTGAANGGCCATTTCCTCNCAATCTTGTGAGTNTGAGGATGTTTTGGTTAATACGAATCANNTNGTTATTCCTTNTCNAATGCTTAGCACATTTGCACATNTANCAGTAAATTANGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4727838_4728531_edi-0_ler-0_2905_11293_TRUE
SEQUENCE_TARGET=950,793
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_6714120_6714543_sf-2_hi-0_248753_1167629_TRUE
SEQUENCE_TARGET=950,523
SEQUENCE_TEMPLATE=TGATGATGGAGTTTTTGTTTGCAGNGGTGGGATATAGCAACANCTTCTGTGAAGTTATNAGANTGTCAAGAAACGATTNCCAGTTTACGGAAGCAACTAAGAGCGTTATCTACAACAGAAACAAGCAGCACCATTAAATTTCTACACAAAAGATCNTCTCTCCNGGAAAATATAGCAGAGGATGATNCTAACAGAGTTGCTCAAGATGATNATGGTAACCGTTACAACGCTTTGATCGTGTACGAACCAGTGAAGGCGAGAGGTGAGAAGATGGAGATGGTACCGAGAAAGAAGCAAGGATTAGGGTTCTTGAAAAAACTGTTGTTCAGGAGGAAAAGAGTGAGCAGCAAGAAATGTCTTGCCTTGACTATGTGATGAAGAAAACTAAACTAAAANGGCTTNTGTGTGTGTAATGAGTGAACAGTGAAAACTGCTTTTGGGTATGTTTGATGTGTCTACTCTATGAGATGTGATGAGTTTAGACTCTTATTTGATCTAATAAACTAAAATAAAAGGAATGTGTTCAATCCACGTTAACCCGGAATGTGAAAAAACCAAACTGAGTTTTTTAAGTTGTTAAAATAATCTTCTTTGATAATCTAACTTATATCAAAACCTGAAAAAAAANTCAAGATTTGCTTTTTCTTCTAGGATCTATAGCTAAAGAAAATTTGAATCAACATTGTGAATGAAAAGTGCAGTTCATATAAGATTGGACTTTATTTTGNTTACTTTTATGTTCTTTTTAGTAGTCTACACCTTCATAGTTCATATACACACACTATCATATTTGAGTCCATACTAACTACTTTATTGTGTTGCANATATTCAGCCATGGNNNNNNNCTAGGCCCAGTTTGTTTATTGTTCATTTGTTTGANNATNTAGCCGATCAATGNTTTCTTGCAAGTTGCAACCATNTAAAGTACTTTTGGGGTCCTTAAAATAGAGACTAATCGAAATGTTAACAANTGCTNTAACACCNTTCAACAANAAAAANNNCNAGTTGCACCCAAAAAATANAAAAAGGAGACACTTAAAAGAAGATAAATNAACAAGAGCTCTACAACACAAAGTACANACTACCNTAATGACGTTCANNNNNNNNNNNNNNNNNNNNNNNAAATTTGCCAACATNTCGATACCAAAATTTACCTGTNNAAATGCANAAAAAAGTTGACTTACCTTTAGNNNNNNNACTTTAAAANGCCTTTAAGCATATCTTCCNTTTGCTTCTTTNACNACTTTTNCCAACTCAAAAGGCCAATCAAATAACAAAATAATTACTTNNNNTANNTACNNNNAAAAAAAAAANCTAATTCATGGTCATCAAAAAGCTTCTGTATATTATCTTTTCTTCTTCACATCTTTCCATCAAATACACACNAAAAATGACCATNNTTTTTCCTTCTAATTNAAAAAAANANNTTTTTTTNATAATTACTGTGAAAAAACANTGTTTNNANAAAAAANAATCGANNNATCAAAAAGGGACCACTTGGCTGCTGCAACCAAAGTCATCGGAGTTAGCGAAGGAATTGTAGTCAGGCCANGAATCTCTCAGAAGCTTCAATAACTTCTGTGCTTTCTTCTTCGCTCTCTCCGTACACTCACTCTGCACCATCAACAACAACTGTACCACCACTCCCGCCCCNGCCGCTTCCTCCCTCCACCTTTCCTCCGCCGTACAAAGCGCTAACAACGCCNCCGCCGCNTACTCCGTCGCGCGATCCGAAACTCTCAATATCGTTTTNACTANCAACGGCACCGTTAAAGCGTGCTCACCGAACGCCGCGCANCCTTCCGGCGTTCGGCAGAGTAGCTCCACCGTCGCTAAGGCTCGCTCCGTATCGCATCTATCGAAATCCGCCGCGAGTCTGTCTATTAGAATCTCCGGCGCACCGGCNGTGATCGCGATGTGTCGCGTNGATTTCACTNAACAGAGNGCGAACANAGTTTTGATTCCGATTTTGAGAGCTCGTCGTGACGAAATCGGATTTCTCAGAAGATCGAGAACTCCTTCGAANANAGANTCNGAATTNGAAATCGANCCTTTCAGGTCCGCCGATTTGGTTCCCNTCGAAACAATCTCGANCAACGCGGCGGCGTTAACGCGTGTTTCAATCGACGAATCGAATAACAACCGGGTCAAAAACTCGACCCGACCCGGATCNGACGATATCGAAACAAACTGGTTCGGCTCCGTTATCGGTAACATAACAAGNAGAGCTAANGACTCAGAGNCTAACTCCGACGACGTCGTTTCAGAGAACAGAATNTTAATCAAAATCTCTGTAGCGTTATGAGCTGCGATCAAAACGCGNTTTTTATCAGAATCACGAGCGAAACCACGTAAACGGCGGAGAGCGGCGGCNCGTGAACGAACAGAGACGTGNGTTCCNGTTATAGCAGAGGCTTGNCTGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.622652
PRIMER_LEFT_0_PENALTY=0.227356
PRIMER_RIGHT_0_PENALTY=1.395296
PRIMER_LEFT_0_SEQUENCE=ACGTTAACCCGGAATGTGAA
PRIMER_RIGHT_0_SEQUENCE=CAGCCAAGTGGTCCCTTTTT
PRIMER_LEFT_0=530,20
PRIMER_RIGHT_0=1503,20
PRIMER_LEFT_0_TM=60.227
PRIMER_RIGHT_0_TM=61.395
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=974
PRIMER_PAIR_1_PENALTY=2.117610
PRIMER_LEFT_1_PENALTY=0.722314
PRIMER_RIGHT_1_PENALTY=1.395296
PRIMER_LEFT_1_SEQUENCE=CGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_1_SEQUENCE=CAGCCAAGTGGTCCCTTTTT
PRIMER_LEFT_1=531,20
PRIMER_RIGHT_1=1503,20
PRIMER_LEFT_1_TM=60.722
PRIMER_RIGHT_1_TM=61.395
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=2.527351
PRIMER_LEFT_2_PENALTY=0.227356
PRIMER_RIGHT_2_PENALTY=2.299995
PRIMER_LEFT_2_SEQUENCE=ACGTTAACCCGGAATGTGAA
PRIMER_RIGHT_2_SEQUENCE=GCCAAGTGGTCCCTTTTTGA
PRIMER_LEFT_2=530,20
PRIMER_RIGHT_2=1501,20
PRIMER_LEFT_2_TM=60.227
PRIMER_RIGHT_2_TM=62.300
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=972
PRIMER_PAIR_3_PENALTY=2.569232
PRIMER_LEFT_3_PENALTY=0.227356
PRIMER_RIGHT_3_PENALTY=2.341875
PRIMER_LEFT_3_SEQUENCE=ACGTTAACCCGGAATGTGAA
PRIMER_RIGHT_3_SEQUENCE=GCTAACTCCGATGACTTTGGTT
PRIMER_LEFT_3=530,20
PRIMER_RIGHT_3=1529,22
PRIMER_LEFT_3_TM=60.227
PRIMER_RIGHT_3_TM=59.658
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=1000
PRIMER_PAIR_4_PENALTY=2.585910
PRIMER_LEFT_4_PENALTY=0.227356
PRIMER_RIGHT_4_PENALTY=2.358553
PRIMER_LEFT_4_SEQUENCE=ACGTTAACCCGGAATGTGAA
PRIMER_RIGHT_4_SEQUENCE=AACTCCGATGACTTTGGTTG
PRIMER_LEFT_4=530,20
PRIMER_RIGHT_4=1526,20
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=57.641
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=997
PRIMER_PAIR_5_PENALTY=2.683950
PRIMER_LEFT_5_PENALTY=2.520292
PRIMER_RIGHT_5_PENALTY=0.163658
PRIMER_LEFT_5_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_5_SEQUENCE=CCTTCGCTAACTCCGATGAC
PRIMER_LEFT_5=848,21
PRIMER_RIGHT_5=1534,20
PRIMER_LEFT_5_TM=58.480
PRIMER_RIGHT_5_TM=59.836
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=687
PRIMER_PAIR_6_PENALTY=2.748162
PRIMER_LEFT_6_PENALTY=2.584504
PRIMER_RIGHT_6_PENALTY=0.163658
PRIMER_LEFT_6_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_6_SEQUENCE=CCTTCGCTAACTCCGATGAC
PRIMER_LEFT_6=847,22
PRIMER_RIGHT_6=1534,20
PRIMER_LEFT_6_TM=59.415
PRIMER_RIGHT_6_TM=59.836
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=688
PRIMER_PAIR_7_PENALTY=2.794039
PRIMER_LEFT_7_PENALTY=2.520292
PRIMER_RIGHT_7_PENALTY=0.273747
PRIMER_LEFT_7_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_7_SEQUENCE=TGAGTGTACGGAGAGAGCGA
PRIMER_LEFT_7=848,21
PRIMER_RIGHT_7=1611,20
PRIMER_LEFT_7_TM=58.480
PRIMER_RIGHT_7_TM=59.726
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=9.9000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=764
PRIMER_PAIR_8_PENALTY=2.858251
PRIMER_LEFT_8_PENALTY=2.584504
PRIMER_RIGHT_8_PENALTY=0.273747
PRIMER_LEFT_8_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_8_SEQUENCE=TGAGTGTACGGAGAGAGCGA
PRIMER_LEFT_8=847,22
PRIMER_RIGHT_8=1611,20
PRIMER_LEFT_8_TM=59.415
PRIMER_RIGHT_8_TM=59.726
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=9.9000
PRIMER_PAIR_8_COMPL_ANY=2.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=765
PRIMER_PAIR_9_PENALTY=2.864242
PRIMER_LEFT_9_PENALTY=2.520292
PRIMER_RIGHT_9_PENALTY=0.343950
PRIMER_LEFT_9_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_9_SEQUENCE=TTCCTTCGCTAACTCCGATG
PRIMER_LEFT_9=848,21
PRIMER_RIGHT_9=1536,20
PRIMER_LEFT_9_TM=58.480
PRIMER_RIGHT_9_TM=60.344
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=689
PRIMER_PAIR_10_PENALTY=2.895564
PRIMER_LEFT_10_PENALTY=0.227356
PRIMER_RIGHT_10_PENALTY=2.668208
PRIMER_LEFT_10_SEQUENCE=ACGTTAACCCGGAATGTGAA
PRIMER_RIGHT_10_SEQUENCE=GCTAACTCCGATGACTTTGGT
PRIMER_LEFT_10=530,20
PRIMER_RIGHT_10=1529,21
PRIMER_LEFT_10_TM=60.227
PRIMER_RIGHT_10_TM=58.332
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=1000
PRIMER_PAIR_11_PENALTY=2.928454
PRIMER_LEFT_11_PENALTY=2.584504
PRIMER_RIGHT_11_PENALTY=0.343950
PRIMER_LEFT_11_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_11_SEQUENCE=TTCCTTCGCTAACTCCGATG
PRIMER_LEFT_11=847,22
PRIMER_RIGHT_11=1536,20
PRIMER_LEFT_11_TM=59.415
PRIMER_RIGHT_11_TM=60.344
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=690
PRIMER_PAIR_12_PENALTY=3.022309
PRIMER_LEFT_12_PENALTY=0.722314
PRIMER_RIGHT_12_PENALTY=2.299995
PRIMER_LEFT_12_SEQUENCE=CGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_12_SEQUENCE=GCCAAGTGGTCCCTTTTTGA
PRIMER_LEFT_12=531,20
PRIMER_RIGHT_12=1501,20
PRIMER_LEFT_12_TM=60.722
PRIMER_RIGHT_12_TM=62.300
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=971
PRIMER_PAIR_13_PENALTY=3.064190
PRIMER_LEFT_13_PENALTY=0.722314
PRIMER_RIGHT_13_PENALTY=2.341875
PRIMER_LEFT_13_SEQUENCE=CGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_13_SEQUENCE=GCTAACTCCGATGACTTTGGTT
PRIMER_LEFT_13=531,20
PRIMER_RIGHT_13=1529,22
PRIMER_LEFT_13_TM=60.722
PRIMER_RIGHT_13_TM=59.658
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=999
PRIMER_PAIR_14_PENALTY=3.080868
PRIMER_LEFT_14_PENALTY=0.722314
PRIMER_RIGHT_14_PENALTY=2.358553
PRIMER_LEFT_14_SEQUENCE=CGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_14_SEQUENCE=AACTCCGATGACTTTGGTTG
PRIMER_LEFT_14=531,20
PRIMER_RIGHT_14=1526,20
PRIMER_LEFT_14_TM=60.722
PRIMER_RIGHT_14_TM=57.641
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=996
PRIMER_PAIR_15_PENALTY=3.111325
PRIMER_LEFT_15_PENALTY=1.716029
PRIMER_RIGHT_15_PENALTY=1.395296
PRIMER_LEFT_15_SEQUENCE=CACGTTAACCCGGAATGTGA
PRIMER_RIGHT_15_SEQUENCE=CAGCCAAGTGGTCCCTTTTT
PRIMER_LEFT_15=529,20
PRIMER_RIGHT_15=1503,20
PRIMER_LEFT_15_TM=61.716
PRIMER_RIGHT_15_TM=61.395
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=975
PRIMER_PAIR_16_PENALTY=3.183971
PRIMER_LEFT_16_PENALTY=2.520292
PRIMER_RIGHT_16_PENALTY=0.663680
PRIMER_LEFT_16_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_16_SEQUENCE=GGTGCAGAGTGAGTGTACGG
PRIMER_LEFT_16=848,21
PRIMER_RIGHT_16=1620,20
PRIMER_LEFT_16_TM=58.480
PRIMER_RIGHT_16_TM=59.336
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=60.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=773
PRIMER_PAIR_17_PENALTY=3.248184
PRIMER_LEFT_17_PENALTY=2.584504
PRIMER_RIGHT_17_PENALTY=0.663680
PRIMER_LEFT_17_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_17_SEQUENCE=GGTGCAGAGTGAGTGTACGG
PRIMER_LEFT_17=847,22
PRIMER_RIGHT_17=1620,20
PRIMER_LEFT_17_TM=59.415
PRIMER_RIGHT_17_TM=59.336
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=60.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=774
PRIMER_PAIR_18_PENALTY=3.389102
PRIMER_LEFT_18_PENALTY=3.369335
PRIMER_RIGHT_18_PENALTY=0.019767
PRIMER_LEFT_18_SEQUENCE=GGCCCAGTTTGTTTATTGTT
PRIMER_RIGHT_18_SEQUENCE=GTGGAGCTACTCTGCCGAAC
PRIMER_LEFT_18=848,20
PRIMER_RIGHT_18=1826,20
PRIMER_LEFT_18_TM=56.631
PRIMER_RIGHT_18_TM=60.020
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=60.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=979
PRIMER_PAIR_19_PENALTY=3.390522
PRIMER_LEFT_19_PENALTY=0.722314
PRIMER_RIGHT_19_PENALTY=2.668208
PRIMER_LEFT_19_SEQUENCE=CGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_19_SEQUENCE=GCTAACTCCGATGACTTTGGT
PRIMER_LEFT_19=531,20
PRIMER_RIGHT_19=1529,21
PRIMER_LEFT_19_TM=60.722
PRIMER_RIGHT_19_TM=58.332
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.3000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=999
PRIMER_PAIR_20_PENALTY=3.393861
PRIMER_LEFT_20_PENALTY=2.520292
PRIMER_RIGHT_20_PENALTY=0.873570
PRIMER_LEFT_20_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_20_SEQUENCE=TCCTTCGCTAACTCCGATGA
PRIMER_LEFT_20=848,21
PRIMER_RIGHT_20=1535,20
PRIMER_LEFT_20_TM=58.480
PRIMER_RIGHT_20_TM=60.874
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=688
PRIMER_PAIR_21_PENALTY=3.458074
PRIMER_LEFT_21_PENALTY=2.584504
PRIMER_RIGHT_21_PENALTY=0.873570
PRIMER_LEFT_21_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_21_SEQUENCE=TCCTTCGCTAACTCCGATGA
PRIMER_LEFT_21=847,22
PRIMER_RIGHT_21=1535,20
PRIMER_LEFT_21_TM=59.415
PRIMER_RIGHT_21_TM=60.874
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=6.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=689
PRIMER_PAIR_22_PENALTY=3.462195
PRIMER_LEFT_22_PENALTY=0.227356
PRIMER_RIGHT_22_PENALTY=3.234839
PRIMER_LEFT_22_SEQUENCE=ACGTTAACCCGGAATGTGAA
PRIMER_RIGHT_22_SEQUENCE=CTAACTCCGATGACTTTGGTTG
PRIMER_LEFT_22=530,20
PRIMER_RIGHT_22=1528,22
PRIMER_LEFT_22_TM=60.227
PRIMER_RIGHT_22_TM=58.765
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.455
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=999
PRIMER_PAIR_23_PENALTY=3.483880
PRIMER_LEFT_23_PENALTY=0.227356
PRIMER_RIGHT_23_PENALTY=3.256524
PRIMER_LEFT_23_SEQUENCE=ACGTTAACCCGGAATGTGAA
PRIMER_RIGHT_23_SEQUENCE=TAACTCCGATGACTTTGGTTG
PRIMER_LEFT_23=530,20
PRIMER_RIGHT_23=1527,21
PRIMER_LEFT_23_TM=60.227
PRIMER_RIGHT_23_TM=57.743
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=998
PRIMER_PAIR_24_PENALTY=3.532993
PRIMER_LEFT_24_PENALTY=3.369335
PRIMER_RIGHT_24_PENALTY=0.163658
PRIMER_LEFT_24_SEQUENCE=GGCCCAGTTTGTTTATTGTT
PRIMER_RIGHT_24_SEQUENCE=CCTTCGCTAACTCCGATGAC
PRIMER_LEFT_24=848,20
PRIMER_RIGHT_24=1534,20
PRIMER_LEFT_24_TM=56.631
PRIMER_RIGHT_24_TM=59.836
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.3000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=687
PRIMER_PAIR_25_PENALTY=3.631596
PRIMER_LEFT_25_PENALTY=2.520292
PRIMER_RIGHT_25_PENALTY=1.111304
PRIMER_LEFT_25_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_25_SEQUENCE=TTGTTGATGGTGCAGAGTGAG
PRIMER_LEFT_25=848,21
PRIMER_RIGHT_25=1628,21
PRIMER_LEFT_25_TM=58.480
PRIMER_RIGHT_25_TM=59.889
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=781
PRIMER_PAIR_26_PENALTY=3.643082
PRIMER_LEFT_26_PENALTY=3.369335
PRIMER_RIGHT_26_PENALTY=0.273747
PRIMER_LEFT_26_SEQUENCE=GGCCCAGTTTGTTTATTGTT
PRIMER_RIGHT_26_SEQUENCE=TGAGTGTACGGAGAGAGCGA
PRIMER_LEFT_26=848,20
PRIMER_RIGHT_26=1611,20
PRIMER_LEFT_26_TM=56.631
PRIMER_RIGHT_26_TM=59.726
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=764
PRIMER_PAIR_27_PENALTY=3.695808
PRIMER_LEFT_27_PENALTY=2.584504
PRIMER_RIGHT_27_PENALTY=1.111304
PRIMER_LEFT_27_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_27_SEQUENCE=TTGTTGATGGTGCAGAGTGAG
PRIMER_LEFT_27=847,22
PRIMER_RIGHT_27=1628,21
PRIMER_LEFT_27_TM=59.415
PRIMER_RIGHT_27_TM=59.889
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=782
PRIMER_PAIR_28_PENALTY=3.713285
PRIMER_LEFT_28_PENALTY=3.369335
PRIMER_RIGHT_28_PENALTY=0.343950
PRIMER_LEFT_28_SEQUENCE=GGCCCAGTTTGTTTATTGTT
PRIMER_RIGHT_28_SEQUENCE=TTCCTTCGCTAACTCCGATG
PRIMER_LEFT_28=848,20
PRIMER_RIGHT_28=1536,20
PRIMER_LEFT_28_TM=56.631
PRIMER_RIGHT_28_TM=60.344
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=8.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=689
PRIMER_PAIR_29_PENALTY=3.735926
PRIMER_LEFT_29_PENALTY=2.520292
PRIMER_RIGHT_29_PENALTY=1.215635
PRIMER_LEFT_29_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_29_SEQUENCE=TTGTTGATGGTGCAGAGTGA
PRIMER_LEFT_29=848,21
PRIMER_RIGHT_29=1628,20
PRIMER_LEFT_29_TM=58.480
PRIMER_RIGHT_29_TM=58.784
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=781
PRIMER_PAIR_30_PENALTY=3.754634
PRIMER_LEFT_30_PENALTY=2.520292
PRIMER_RIGHT_30_PENALTY=1.234342
PRIMER_LEFT_30_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_30_SEQUENCE=TGTACGGAGAGAGCGAAGAAG
PRIMER_LEFT_30=848,21
PRIMER_RIGHT_30=1607,21
PRIMER_LEFT_30_TM=58.480
PRIMER_RIGHT_30_TM=59.766
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=760
PRIMER_PAIR_31_PENALTY=3.778218
PRIMER_LEFT_31_PENALTY=2.520292
PRIMER_RIGHT_31_PENALTY=1.257926
PRIMER_LEFT_31_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_31_SEQUENCE=GTTGTTGATGGTGCAGAGTGA
PRIMER_LEFT_31=848,21
PRIMER_RIGHT_31=1629,21
PRIMER_LEFT_31_TM=58.480
PRIMER_RIGHT_31_TM=59.742
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=782
PRIMER_PAIR_32_PENALTY=3.782400
PRIMER_LEFT_32_PENALTY=3.762633
PRIMER_RIGHT_32_PENALTY=0.019767
PRIMER_LEFT_32_SEQUENCE=AGGCCCAGTTTGTTTATTGT
PRIMER_RIGHT_32_SEQUENCE=GTGGAGCTACTCTGCCGAAC
PRIMER_LEFT_32=847,20
PRIMER_RIGHT_32=1826,20
PRIMER_LEFT_32_TM=56.237
PRIMER_RIGHT_32_TM=60.020
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=60.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=980
PRIMER_PAIR_33_PENALTY=3.800139
PRIMER_LEFT_33_PENALTY=2.584504
PRIMER_RIGHT_33_PENALTY=1.215635
PRIMER_LEFT_33_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_33_SEQUENCE=TTGTTGATGGTGCAGAGTGA
PRIMER_LEFT_33=847,22
PRIMER_RIGHT_33=1628,20
PRIMER_LEFT_33_TM=59.415
PRIMER_RIGHT_33_TM=58.784
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=782
PRIMER_PAIR_34_PENALTY=3.818846
PRIMER_LEFT_34_PENALTY=2.584504
PRIMER_RIGHT_34_PENALTY=1.234342
PRIMER_LEFT_34_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_34_SEQUENCE=TGTACGGAGAGAGCGAAGAAG
PRIMER_LEFT_34=847,22
PRIMER_RIGHT_34=1607,21
PRIMER_LEFT_34_TM=59.415
PRIMER_RIGHT_34_TM=59.766
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=761
PRIMER_PAIR_35_PENALTY=3.842430
PRIMER_LEFT_35_PENALTY=2.584504
PRIMER_RIGHT_35_PENALTY=1.257926
PRIMER_LEFT_35_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_35_SEQUENCE=GTTGTTGATGGTGCAGAGTGA
PRIMER_LEFT_35=847,22
PRIMER_RIGHT_35=1629,21
PRIMER_LEFT_35_TM=59.415
PRIMER_RIGHT_35_TM=59.742
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=783
PRIMER_PAIR_36_PENALTY=3.870857
PRIMER_LEFT_36_PENALTY=2.475561
PRIMER_RIGHT_36_PENALTY=1.395296
PRIMER_LEFT_36_SEQUENCE=ACGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_36_SEQUENCE=CAGCCAAGTGGTCCCTTTTT
PRIMER_LEFT_36=530,21
PRIMER_RIGHT_36=1503,20
PRIMER_LEFT_36_TM=61.476
PRIMER_RIGHT_36_TM=61.395
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=974
PRIMER_PAIR_37_PENALTY=3.915588
PRIMER_LEFT_37_PENALTY=2.520292
PRIMER_RIGHT_37_PENALTY=1.395296
PRIMER_LEFT_37_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_37_SEQUENCE=CAGCCAAGTGGTCCCTTTTT
PRIMER_LEFT_37=848,21
PRIMER_RIGHT_37=1503,20
PRIMER_LEFT_37_TM=58.480
PRIMER_RIGHT_37_TM=61.395
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=656
PRIMER_PAIR_38_PENALTY=3.926291
PRIMER_LEFT_38_PENALTY=3.762633
PRIMER_RIGHT_38_PENALTY=0.163658
PRIMER_LEFT_38_SEQUENCE=AGGCCCAGTTTGTTTATTGT
PRIMER_RIGHT_38_SEQUENCE=CCTTCGCTAACTCCGATGAC
PRIMER_LEFT_38=847,20
PRIMER_RIGHT_38=1534,20
PRIMER_LEFT_38_TM=56.237
PRIMER_RIGHT_38_TM=59.836
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=688
PRIMER_PAIR_39_PENALTY=3.957153
PRIMER_LEFT_39_PENALTY=0.722314
PRIMER_RIGHT_39_PENALTY=3.234839
PRIMER_LEFT_39_SEQUENCE=CGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_39_SEQUENCE=CTAACTCCGATGACTTTGGTTG
PRIMER_LEFT_39=531,20
PRIMER_RIGHT_39=1528,22
PRIMER_LEFT_39_TM=60.722
PRIMER_RIGHT_39_TM=58.765
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=998
PRIMER_PAIR_40_PENALTY=3.978838
PRIMER_LEFT_40_PENALTY=0.722314
PRIMER_RIGHT_40_PENALTY=3.256524
PRIMER_LEFT_40_SEQUENCE=CGTTAACCCGGAATGTGAAA
PRIMER_RIGHT_40_SEQUENCE=TAACTCCGATGACTTTGGTTG
PRIMER_LEFT_40=531,20
PRIMER_RIGHT_40=1527,21
PRIMER_LEFT_40_TM=60.722
PRIMER_RIGHT_40_TM=57.743
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.3000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=997
PRIMER_PAIR_41_PENALTY=3.979800
PRIMER_LEFT_41_PENALTY=2.584504
PRIMER_RIGHT_41_PENALTY=1.395296
PRIMER_LEFT_41_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_41_SEQUENCE=CAGCCAAGTGGTCCCTTTTT
PRIMER_LEFT_41=847,22
PRIMER_RIGHT_41=1503,20
PRIMER_LEFT_41_TM=59.415
PRIMER_RIGHT_41_TM=61.395
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.6000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=657
PRIMER_PAIR_42_PENALTY=4.005345
PRIMER_LEFT_42_PENALTY=2.520292
PRIMER_RIGHT_42_PENALTY=1.485053
PRIMER_LEFT_42_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_42_SEQUENCE=GTTGTTAGCGCTTTGTACGG
PRIMER_LEFT_42=848,21
PRIMER_RIGHT_42=1701,20
PRIMER_LEFT_42_TM=58.480
PRIMER_RIGHT_42_TM=58.515
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=8.9000
PRIMER_PAIR_42_COMPL_ANY=2.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=854
PRIMER_PAIR_43_PENALTY=4.016024
PRIMER_LEFT_43_PENALTY=1.716029
PRIMER_RIGHT_43_PENALTY=2.299995
PRIMER_LEFT_43_SEQUENCE=CACGTTAACCCGGAATGTGA
PRIMER_RIGHT_43_SEQUENCE=GCCAAGTGGTCCCTTTTTGA
PRIMER_LEFT_43=529,20
PRIMER_RIGHT_43=1501,20
PRIMER_LEFT_43_TM=61.716
PRIMER_RIGHT_43_TM=62.300
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.3000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=973
PRIMER_PAIR_44_PENALTY=4.033015
PRIMER_LEFT_44_PENALTY=3.369335
PRIMER_RIGHT_44_PENALTY=0.663680
PRIMER_LEFT_44_SEQUENCE=GGCCCAGTTTGTTTATTGTT
PRIMER_RIGHT_44_SEQUENCE=GGTGCAGAGTGAGTGTACGG
PRIMER_LEFT_44=848,20
PRIMER_RIGHT_44=1620,20
PRIMER_LEFT_44_TM=56.631
PRIMER_RIGHT_44_TM=59.336
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=60.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=8.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=773
PRIMER_PAIR_45_PENALTY=4.036380
PRIMER_LEFT_45_PENALTY=3.762633
PRIMER_RIGHT_45_PENALTY=0.273747
PRIMER_LEFT_45_SEQUENCE=AGGCCCAGTTTGTTTATTGT
PRIMER_RIGHT_45_SEQUENCE=TGAGTGTACGGAGAGAGCGA
PRIMER_LEFT_45=847,20
PRIMER_RIGHT_45=1611,20
PRIMER_LEFT_45_TM=56.237
PRIMER_RIGHT_45_TM=59.726
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.6000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=2.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=765
PRIMER_PAIR_46_PENALTY=4.069557
PRIMER_LEFT_46_PENALTY=2.584504
PRIMER_RIGHT_46_PENALTY=1.485053
PRIMER_LEFT_46_SEQUENCE=AGGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_46_SEQUENCE=GTTGTTAGCGCTTTGTACGG
PRIMER_LEFT_46=847,22
PRIMER_RIGHT_46=1701,20
PRIMER_LEFT_46_TM=59.415
PRIMER_RIGHT_46_TM=58.515
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.9000
PRIMER_PAIR_46_COMPL_ANY=2.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=855
PRIMER_PAIR_47_PENALTY=4.074582
PRIMER_LEFT_47_PENALTY=1.716029
PRIMER_RIGHT_47_PENALTY=2.358553
PRIMER_LEFT_47_SEQUENCE=CACGTTAACCCGGAATGTGA
PRIMER_RIGHT_47_SEQUENCE=AACTCCGATGACTTTGGTTG
PRIMER_LEFT_47=529,20
PRIMER_RIGHT_47=1526,20
PRIMER_LEFT_47_TM=61.716
PRIMER_RIGHT_47_TM=57.641
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=998
PRIMER_PAIR_48_PENALTY=4.106583
PRIMER_LEFT_48_PENALTY=3.762633
PRIMER_RIGHT_48_PENALTY=0.343950
PRIMER_LEFT_48_SEQUENCE=AGGCCCAGTTTGTTTATTGT
PRIMER_RIGHT_48_SEQUENCE=TTCCTTCGCTAACTCCGATG
PRIMER_LEFT_48=847,20
PRIMER_RIGHT_48=1536,20
PRIMER_LEFT_48_TM=56.237
PRIMER_RIGHT_48_TM=60.344
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=8.6000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=690
PRIMER_PAIR_49_PENALTY=4.107552
PRIMER_LEFT_49_PENALTY=2.520292
PRIMER_RIGHT_49_PENALTY=1.587260
PRIMER_LEFT_49_SEQUENCE=GGCCCAGTTTGTTTATTGTTC
PRIMER_RIGHT_49_SEQUENCE=ATTCCTTCGCTAACTCCGATG
PRIMER_LEFT_49=848,21
PRIMER_RIGHT_49=1537,21
PRIMER_LEFT_49_TM=58.480
PRIMER_RIGHT_49_TM=60.587
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.6000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=690
=
SEQUENCE_ID=MAGIC.105_2_2638291_2638334_can-0_wil-2_16283_58260_TRUE
SEQUENCE_TARGET=950,143
SEQUENCE_TEMPLATE=TTGGTNGGAANTTTTGTCGGAAAATACAATGTTTTCTTGTAGTATANAGCNANNCATTATCATCTTTTNTTNTATTTGCAGATGATTTAGATGTCTTTTGANNNNNNTTTCCTGTGNTTTAATTTAAGAGTTGTNNGAGAAGAAGTNNNNTANNNNNNNNNNNNNNNNNNNNNNNNATTTNTTAGTTTTTATGGANTGCTGTCGAATTTNAATATTTANAAAAATATTATTNGACNNNNNNNNNNNNNNNTTGGTGTATTGAAANATCGTTTATAACCAATGTACCTTTAAGCTTTCCAGTAGCTCTTTTCGCCAATAGTATTGCATGGAGCATTTGTCNGGNTTTTCTTTGTTCCTCTNCAAATATATAATNNTAGTATTGTTCCATTTGTTAAATGTAGTTTACTATTATTTGCNGACNTATTANCTTGAATANCCAAAAAANTTNNNNNNNNTATATTTTATTTATATATNTATCCANANAACTTNGTTTNTAGTCGTTTNGTTTNNNTCGGCGTACNCNGATGTTTGACATGTAANNTGTACTCTGAANTGGAGAATNTNTATAGGAATAAAGTAAGTTTNNTNGTTTATTTTTNAANGTAGTGATCNTAAATGCATTATNTGATACTATTATTGAGAGATGGTAANCTTACTTTGTCTTTGATTGGTGCATTGTGGTTCTTCATGAGTNTNTTTTTTTCTTTNNNTTTTNGCTGCNTGAGTTGAAGTTTGTNAGTANCCTTTTCTNNNNCCTAAANGTTCTCGCTTCATNANTTTTANTTNTTTTTTTACTTGATTTCTTTATTTNNTANCATCNGTTTNTGGTTTTATTTGGTTTTNTTATNAGANTGTTGTNTTTANTGTAATGATGTAGTNAAAANNTTGTAATAGTGAGACGAATNCTTTTCGTGTTCNTTTGTTTTGTTTAGTATAAGTTTNAAGTATAACCAANATTAAATTCTCCANANTNTTNTTTAGTTNATNAGTGGTTATATCNNCNATAGATTAAGGGTCTTGTNATTTAGATGTTTTACGTTACANATACGAATTTATTGTCTTNGGTATTAATATATTTTCAAAAACTAATAATATTGAACTCCTCTTANTTCTAAGAAANTGNAGTCTTNGGGAAATCTTTTANATTAGNTTGTCNTTATTATNATCCGGTANATATNTTTNNAATNGAATTACATGAATACATTTGTCAANTTATTCCTTTTANTTATTTATTTTAACAATATTNTAATAGCTACTTGACAAATCNNAANTAGTNAAATTNCNNAATGTCTTCANNTTTTTTTGGGTTGNCTCTTTGGNNNNNNNNNNNGGCAACGTCNTNGCTATTTCGNTTCNTAAAANTATGATTATATNGCTGNTAAATAAATGTAAGAGTTNNANTTTATTTGATTAANAAACTTNCTTANAACNTNAAGGAATANAAACTGCATAGAAAAAGAAATAGTNANAAAAANANAAATAACAAAAACAGCTTTNCTTTGACANTGTGATANCCCACAACTCTATAAGCNTTAAATTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_15988620_15988841_wil-2_tsu-0_115944_1686_TRUE
SEQUENCE_TARGET=950,321
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGTGTGGTTNTAATGCTCATATGACTTGCTACNTTCAGAAGACCNAAAAATATANGNTTGTGTNATTCGAGNNNAACCATAACNATGATTTAGTGAGGACACCTATNAAGCNNNNNNNNNNNNNNNATCNNGAATTATCTATNTCTCNAAAACAACATGNAGATGATGCCGAGATGTNANGGANTNCAGCAAAAGCAACCGTTGAAATGATGAGNANAGAAGTTGGGNTCNGAGTNAATNNNNNNNTTNNNNNAAGAAAGATTATCAAAATTANATATACNNAAAGAGAATNGCAAAAATGGAAAAGNGAGATGNTGGAGCTGTTTTGGAATACTTTCAGAAAANNANGGTAATTCNTTTTTTTTATTCAATGCAANTTGATGANGATGATATGATCACTAATNTATTNTGGGNAGATNATCGGTCTATAAGTGATTACAATCNTTTTGGAGATGTCATTTGCTTTGACACAACTTATAAGACCAATGAATATGATAGANCCTTTGCTCCNTTTATCGGGGTCAATCATCACANGNAAACAGTTGTGTTNNGNGCTGCTCTCTTATATGATGAAACCAGTGAGTCATATAAGTGGNTTTTCGAGACTNTTCTTGGAGCAATTTCTNGAAANCAANCAAAAACAATTNTTACAGACCAATGTGCNNCAATGNCNAATACAATAGTAANTNNNNTNCCTGANACAAAANATAGATTATGTGTTNGACATATTTANCAAAATGATGCNAAGAAGTTGAGTCATGTANTTCNTGGAGCNNAACAATTTGCCATNGATTTTGGTAAGTGTGTATATNANCATGAGGAANAAGAAGACTNGTTATTGGCATGGAGTAATATGNTCCAGAAGCATAACTTGANANATGATAAATNNTTGNAGANTTTGTTTGANCTGAGAGAAAAATGTGCAATGGTATATGGACGTCATACTTTTACAGNAGATATNGTGAGCACACAACGTAGTAAAAGTATGAATAATATT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_21324738_21324755_sf-2_ws-0_60442_65489_TRUE
SEQUENCE_TARGET=950,117
SEQUENCE_TEMPLATE=CTCCTACTGCGGAGAAACTACAAGCACAAGGTGGTAATCAAGGAGATTCATGGGACGATGGTGTTTTCGAAGGTGTTAGAAAGTTNTACNTAGGACAANGTAAAAATTGTGTCGCNTTCCTTAAGNTTGTGTACGATAGCAACACTCAGGTTNTTATTGGAGAAGATCATGGNAACAAAACACTATTTGAAGTCAAGGAGGTGATTANNTATTACTTTNGATGTTTTTATTTTATTTTTTGGGTTTGAGTTTATTATCTTGACATTTTACATTGATTCATTANNTTTTTTNGCAGTACGAGCTTGAATATCCAAGTGAATACATTANAGCCGTGGATGGTTNTTANNATAAAGTAAATGGAACTGAAGTCGAAGTTATAACCATGCTCAGGATTCAAACGAACAAACGAACATCTATACCCGTTGGATGTGAATCTAACTCTAGCTTTGTACTNAAAAAAGAAGGTTACAAAATTGTTGGATTCCATGGGAAAGCAAGTAACATGATTAATCAGCTTGGAGTCCATNTCNTGCCCTTGACTGAGTGATCAANCTTNTTATCTNCAATGTTGTCTTANTTCTNCAATCTCACTTGTATTAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTTTNGTAATTGANCANTCTCCNTATACATACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAGAGAGAAAATNAAATTATAGTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAATTACGTCTATAAATGNATCATTACAAAAACNCACNGAAAATNACATCAAAAGNNNNAAGCAAAAGCNAAAGTCCNAATTTCTCCTGTCATGGACACCTTACCTCATCATCTCCTCGACGAGANTCTNTTCAGAATAGACCACAGATCTNTGGCCATGATACAATGCACAAATAGATCTCTTCAATCTCATATATCCAAAANCCCTAACTTTGCATCCGAATACTTNTCTNGGNTCAGATCNAGTTTGCTNTACATNGCAACCCATGGCTNCCCTTTGATCTGCTACCACCTCTACGGTGATTCTAGGTCACCGCGNACAGTAGAAACCTTGACGCAGTGTCACATTTTAGGCTATTGCTCTGGCCTTCTTCTACTCTTCCTNAATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACTCTAGGTCAAAGTTTTTCCCTNNGGNTNTTACNCTNCGANGAGGTCGTCAACAAGTCATCGGGTTCGCCGTTGATCAGACTGATCGAACCACCCCGAGTTTCAAAATCGTCAACATAAAAGAGGTGANAAATGCCACTNAAACAACATATGAGTTTGAGANTNAACGCANGAGATTCATGGAAANTATTGNAAACGACNTTNACTTGCTGTACAAGTAATCTTGATGACCNTATGAAAAAACCTGTTTATATGAAGGGAGCTNTTCACTGGCTAAGAAATGACGGATCTANCNTAGCTTTCAACCTAAAANCAGAGAAAGCACGA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.424023
PRIMER_LEFT_0_PENALTY=0.201241
PRIMER_RIGHT_0_PENALTY=0.222782
PRIMER_LEFT_0_SEQUENCE=CATGCTCAGGATTCAAACGA
PRIMER_RIGHT_0_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_0=381,20
PRIMER_RIGHT_0=1371,20
PRIMER_LEFT_0_TM=59.799
PRIMER_RIGHT_0_TM=60.223
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=7.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=991
PRIMER_PAIR_1_PENALTY=1.238420
PRIMER_LEFT_1_PENALTY=1.015638
PRIMER_RIGHT_1_PENALTY=0.222782
PRIMER_LEFT_1_SEQUENCE=GATTCCATGGGAAAGCAAGT
PRIMER_RIGHT_1_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_1=479,20
PRIMER_RIGHT_1=1371,20
PRIMER_LEFT_1_TM=58.984
PRIMER_RIGHT_1_TM=60.223
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=893
PRIMER_PAIR_2_PENALTY=1.372681
PRIMER_LEFT_2_PENALTY=1.149900
PRIMER_RIGHT_2_PENALTY=0.222782
PRIMER_LEFT_2_SEQUENCE=GCTCAGGATTCAAACGAACA
PRIMER_RIGHT_2_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_2=384,20
PRIMER_RIGHT_2=1371,20
PRIMER_LEFT_2_TM=58.850
PRIMER_RIGHT_2_TM=60.223
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=988
PRIMER_PAIR_3_PENALTY=1.372681
PRIMER_LEFT_3_PENALTY=1.149900
PRIMER_RIGHT_3_PENALTY=0.222782
PRIMER_LEFT_3_SEQUENCE=TGCTCAGGATTCAAACGAAC
PRIMER_RIGHT_3_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_3=383,20
PRIMER_RIGHT_3=1371,20
PRIMER_LEFT_3_TM=58.850
PRIMER_RIGHT_3_TM=60.223
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=989
PRIMER_PAIR_4_PENALTY=1.464865
PRIMER_LEFT_4_PENALTY=1.242083
PRIMER_RIGHT_4_PENALTY=0.222782
PRIMER_LEFT_4_SEQUENCE=GCTCAGGATTCAAACGAACAA
PRIMER_RIGHT_4_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_4=384,21
PRIMER_RIGHT_4=1371,20
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=60.223
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=988
PRIMER_PAIR_5_PENALTY=1.536341
PRIMER_LEFT_5_PENALTY=0.201241
PRIMER_RIGHT_5_PENALTY=1.335100
PRIMER_LEFT_5_SEQUENCE=CATGCTCAGGATTCAAACGA
PRIMER_RIGHT_5_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_5=381,20
PRIMER_RIGHT_5=1372,20
PRIMER_LEFT_5_TM=59.799
PRIMER_RIGHT_5_TM=61.335
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=992
PRIMER_PAIR_6_PENALTY=1.599484
PRIMER_LEFT_6_PENALTY=1.376703
PRIMER_RIGHT_6_PENALTY=0.222782
PRIMER_LEFT_6_SEQUENCE=CCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_6_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_6=483,20
PRIMER_RIGHT_6=1371,20
PRIMER_LEFT_6_TM=58.623
PRIMER_RIGHT_6_TM=60.223
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.0000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=889
PRIMER_PAIR_7_PENALTY=1.624120
PRIMER_LEFT_7_PENALTY=1.401339
PRIMER_RIGHT_7_PENALTY=0.222782
PRIMER_LEFT_7_SEQUENCE=GGATTCAAACGAACAAACGA
PRIMER_RIGHT_7_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_7=389,20
PRIMER_RIGHT_7=1371,20
PRIMER_LEFT_7_TM=58.599
PRIMER_RIGHT_7_TM=60.223
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.4000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=983
PRIMER_PAIR_8_PENALTY=1.709878
PRIMER_LEFT_8_PENALTY=1.487097
PRIMER_RIGHT_8_PENALTY=0.222782
PRIMER_LEFT_8_SEQUENCE=TCCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_8_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_8=482,21
PRIMER_RIGHT_8=1371,20
PRIMER_LEFT_8_TM=60.487
PRIMER_RIGHT_8_TM=60.223
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=890
PRIMER_PAIR_9_PENALTY=1.929002
PRIMER_LEFT_9_PENALTY=1.706220
PRIMER_RIGHT_9_PENALTY=0.222782
PRIMER_LEFT_9_SEQUENCE=GGATTCCATGGGAAAGCAAG
PRIMER_RIGHT_9_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_9=478,20
PRIMER_RIGHT_9=1371,20
PRIMER_LEFT_9_TM=61.706
PRIMER_RIGHT_9_TM=60.223
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=894
PRIMER_PAIR_10_PENALTY=1.972390
PRIMER_LEFT_10_PENALTY=1.015638
PRIMER_RIGHT_10_PENALTY=0.956752
PRIMER_LEFT_10_SEQUENCE=GATTCCATGGGAAAGCAAGT
PRIMER_RIGHT_10_SEQUENCE=ATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_10=479,20
PRIMER_RIGHT_10=1473,20
PRIMER_LEFT_10_TM=58.984
PRIMER_RIGHT_10_TM=59.043
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=995
PRIMER_PAIR_11_PENALTY=2.008737
PRIMER_LEFT_11_PENALTY=1.785955
PRIMER_RIGHT_11_PENALTY=0.222782
PRIMER_LEFT_11_SEQUENCE=TTCCATGGGAAAGCAAGTAA
PRIMER_RIGHT_11_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_11=481,20
PRIMER_RIGHT_11=1371,20
PRIMER_LEFT_11_TM=58.214
PRIMER_RIGHT_11_TM=60.223
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=891
PRIMER_PAIR_12_PENALTY=2.034566
PRIMER_LEFT_12_PENALTY=1.811785
PRIMER_RIGHT_12_PENALTY=0.222782
PRIMER_LEFT_12_SEQUENCE=ATGCTCAGGATTCAAACGAAC
PRIMER_RIGHT_12_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_12=382,21
PRIMER_RIGHT_12=1371,20
PRIMER_LEFT_12_TM=59.188
PRIMER_RIGHT_12_TM=60.223
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=990
PRIMER_PAIR_13_PENALTY=2.333454
PRIMER_LEFT_13_PENALTY=1.376703
PRIMER_RIGHT_13_PENALTY=0.956752
PRIMER_LEFT_13_SEQUENCE=CCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_13_SEQUENCE=ATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_13=483,20
PRIMER_RIGHT_13=1473,20
PRIMER_LEFT_13_TM=58.623
PRIMER_RIGHT_13_TM=59.043
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.0000
PRIMER_RIGHT_13_END_STABILITY=8.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=991
PRIMER_PAIR_14_PENALTY=2.350738
PRIMER_LEFT_14_PENALTY=1.015638
PRIMER_RIGHT_14_PENALTY=1.335100
PRIMER_LEFT_14_SEQUENCE=GATTCCATGGGAAAGCAAGT
PRIMER_RIGHT_14_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_14=479,20
PRIMER_RIGHT_14=1372,20
PRIMER_LEFT_14_TM=58.984
PRIMER_RIGHT_14_TM=61.335
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=894
PRIMER_PAIR_15_PENALTY=2.358641
PRIMER_LEFT_15_PENALTY=2.135860
PRIMER_RIGHT_15_PENALTY=0.222782
PRIMER_LEFT_15_SEQUENCE=TACCCGTTGGATGTGAATCT
PRIMER_RIGHT_15_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_15=416,20
PRIMER_RIGHT_15=1371,20
PRIMER_LEFT_15_TM=57.864
PRIMER_RIGHT_15_TM=60.223
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=956
PRIMER_PAIR_16_PENALTY=2.443848
PRIMER_LEFT_16_PENALTY=1.487097
PRIMER_RIGHT_16_PENALTY=0.956752
PRIMER_LEFT_16_SEQUENCE=TCCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_16_SEQUENCE=ATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_16=482,21
PRIMER_RIGHT_16=1473,20
PRIMER_LEFT_16_TM=60.487
PRIMER_RIGHT_16_TM=59.043
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.0000
PRIMER_RIGHT_16_END_STABILITY=8.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=992
PRIMER_PAIR_17_PENALTY=2.448755
PRIMER_LEFT_17_PENALTY=0.201241
PRIMER_RIGHT_17_PENALTY=2.247514
PRIMER_LEFT_17_SEQUENCE=CATGCTCAGGATTCAAACGA
PRIMER_RIGHT_17_SEQUENCE=CGAGGAGATGATGAGGTAAGG
PRIMER_LEFT_17=381,20
PRIMER_RIGHT_17=1368,21
PRIMER_LEFT_17_TM=59.799
PRIMER_RIGHT_17_TM=58.752
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=988
PRIMER_PAIR_18_PENALTY=2.485000
PRIMER_LEFT_18_PENALTY=1.149900
PRIMER_RIGHT_18_PENALTY=1.335100
PRIMER_LEFT_18_SEQUENCE=GCTCAGGATTCAAACGAACA
PRIMER_RIGHT_18_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_18=384,20
PRIMER_RIGHT_18=1372,20
PRIMER_LEFT_18_TM=58.850
PRIMER_RIGHT_18_TM=61.335
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=989
PRIMER_PAIR_19_PENALTY=2.485000
PRIMER_LEFT_19_PENALTY=1.149900
PRIMER_RIGHT_19_PENALTY=1.335100
PRIMER_LEFT_19_SEQUENCE=TGCTCAGGATTCAAACGAAC
PRIMER_RIGHT_19_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_19=383,20
PRIMER_RIGHT_19=1372,20
PRIMER_LEFT_19_TM=58.850
PRIMER_RIGHT_19_TM=61.335
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=990
PRIMER_PAIR_20_PENALTY=2.515196
PRIMER_LEFT_20_PENALTY=2.292414
PRIMER_RIGHT_20_PENALTY=0.222782
PRIMER_LEFT_20_SEQUENCE=CATCTATACCCGTTGGATGTGA
PRIMER_RIGHT_20_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_20=410,22
PRIMER_RIGHT_20=1371,20
PRIMER_LEFT_20_TM=59.708
PRIMER_RIGHT_20_TM=60.223
PRIMER_LEFT_20_GC_PERCENT=45.455
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=7.6000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=962
PRIMER_PAIR_21_PENALTY=2.534959
PRIMER_LEFT_21_PENALTY=2.312177
PRIMER_RIGHT_21_PENALTY=0.222782
PRIMER_LEFT_21_SEQUENCE=GATTCCATGGGAAAGCAAGTAA
PRIMER_RIGHT_21_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_21=479,22
PRIMER_RIGHT_21=1371,20
PRIMER_LEFT_21_TM=60.312
PRIMER_RIGHT_21_TM=60.223
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=5.7000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=893
PRIMER_PAIR_22_PENALTY=2.577183
PRIMER_LEFT_22_PENALTY=1.242083
PRIMER_RIGHT_22_PENALTY=1.335100
PRIMER_LEFT_22_SEQUENCE=GCTCAGGATTCAAACGAACAA
PRIMER_RIGHT_22_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_22=384,21
PRIMER_RIGHT_22=1372,20
PRIMER_LEFT_22_TM=60.242
PRIMER_RIGHT_22_TM=61.335
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=989
PRIMER_PAIR_23_PENALTY=2.602602
PRIMER_LEFT_23_PENALTY=0.201241
PRIMER_RIGHT_23_PENALTY=2.401361
PRIMER_LEFT_23_SEQUENCE=CATGCTCAGGATTCAAACGA
PRIMER_RIGHT_23_SEQUENCE=CGAGGAGATGATGAGGTAAGGT
PRIMER_LEFT_23=381,20
PRIMER_RIGHT_23=1368,22
PRIMER_LEFT_23_TM=59.799
PRIMER_RIGHT_23_TM=59.599
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=988
PRIMER_PAIR_24_PENALTY=2.662972
PRIMER_LEFT_24_PENALTY=1.706220
PRIMER_RIGHT_24_PENALTY=0.956752
PRIMER_LEFT_24_SEQUENCE=GGATTCCATGGGAAAGCAAG
PRIMER_RIGHT_24_SEQUENCE=ATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_24=478,20
PRIMER_RIGHT_24=1473,20
PRIMER_LEFT_24_TM=61.706
PRIMER_RIGHT_24_TM=59.043
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=8.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=996
PRIMER_PAIR_25_PENALTY=2.711803
PRIMER_LEFT_25_PENALTY=1.376703
PRIMER_RIGHT_25_PENALTY=1.335100
PRIMER_LEFT_25_SEQUENCE=CCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_25_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_25=483,20
PRIMER_RIGHT_25=1372,20
PRIMER_LEFT_25_TM=58.623
PRIMER_RIGHT_25_TM=61.335
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.0000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=890
PRIMER_PAIR_26_PENALTY=2.736439
PRIMER_LEFT_26_PENALTY=1.401339
PRIMER_RIGHT_26_PENALTY=1.335100
PRIMER_LEFT_26_SEQUENCE=GGATTCAAACGAACAAACGA
PRIMER_RIGHT_26_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_26=389,20
PRIMER_RIGHT_26=1372,20
PRIMER_LEFT_26_TM=58.599
PRIMER_RIGHT_26_TM=61.335
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=984
PRIMER_PAIR_27_PENALTY=2.742707
PRIMER_LEFT_27_PENALTY=1.785955
PRIMER_RIGHT_27_PENALTY=0.956752
PRIMER_LEFT_27_SEQUENCE=TTCCATGGGAAAGCAAGTAA
PRIMER_RIGHT_27_SEQUENCE=ATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_27=481,20
PRIMER_RIGHT_27=1473,20
PRIMER_LEFT_27_TM=58.214
PRIMER_RIGHT_27_TM=59.043
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.7000
PRIMER_RIGHT_27_END_STABILITY=8.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=993
PRIMER_PAIR_28_PENALTY=2.790903
PRIMER_LEFT_28_PENALTY=0.201241
PRIMER_RIGHT_28_PENALTY=2.589662
PRIMER_LEFT_28_SEQUENCE=CATGCTCAGGATTCAAACGA
PRIMER_RIGHT_28_SEQUENCE=TCGAGGAGATGATGAGGTAAGG
PRIMER_LEFT_28=381,20
PRIMER_RIGHT_28=1369,22
PRIMER_LEFT_28_TM=59.799
PRIMER_RIGHT_28_TM=60.590
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=7.5000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=989
PRIMER_PAIR_29_PENALTY=2.822197
PRIMER_LEFT_29_PENALTY=1.487097
PRIMER_RIGHT_29_PENALTY=1.335100
PRIMER_LEFT_29_SEQUENCE=TCCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_29_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_29=482,21
PRIMER_RIGHT_29=1372,20
PRIMER_LEFT_29_TM=60.487
PRIMER_RIGHT_29_TM=61.335
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.0000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=891
PRIMER_PAIR_30_PENALTY=2.927883
PRIMER_LEFT_30_PENALTY=1.015638
PRIMER_RIGHT_30_PENALTY=1.912245
PRIMER_LEFT_30_SEQUENCE=GATTCCATGGGAAAGCAAGT
PRIMER_RIGHT_30_SEQUENCE=TATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_30=479,20
PRIMER_RIGHT_30=1474,21
PRIMER_LEFT_30_TM=58.984
PRIMER_RIGHT_30_TM=59.088
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=8.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=996
PRIMER_PAIR_31_PENALTY=2.954957
PRIMER_LEFT_31_PENALTY=2.732175
PRIMER_RIGHT_31_PENALTY=0.222782
PRIMER_LEFT_31_SEQUENCE=TGCTCAGGATTCAAACGAACA
PRIMER_RIGHT_31_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_31=383,21
PRIMER_RIGHT_31=1371,20
PRIMER_LEFT_31_TM=61.732
PRIMER_RIGHT_31_TM=60.223
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=989
PRIMER_PAIR_32_PENALTY=2.963347
PRIMER_LEFT_32_PENALTY=2.740565
PRIMER_RIGHT_32_PENALTY=0.222782
PRIMER_LEFT_32_SEQUENCE=GGATTCAAACGAACAAACGAAC
PRIMER_RIGHT_32_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_32=389,22
PRIMER_RIGHT_32=1371,20
PRIMER_LEFT_32_TM=60.741
PRIMER_RIGHT_32_TM=60.223
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=983
PRIMER_PAIR_33_PENALTY=2.969096
PRIMER_LEFT_33_PENALTY=2.746314
PRIMER_RIGHT_33_PENALTY=0.222782
PRIMER_LEFT_33_SEQUENCE=ATACCCGTTGGATGTGAATCT
PRIMER_RIGHT_33_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_33=415,21
PRIMER_RIGHT_33=1371,20
PRIMER_LEFT_33_TM=58.254
PRIMER_RIGHT_33_TM=60.223
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.6000
PRIMER_RIGHT_33_END_STABILITY=7.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=957
PRIMER_PAIR_34_PENALTY=3.041320
PRIMER_LEFT_34_PENALTY=1.706220
PRIMER_RIGHT_34_PENALTY=1.335100
PRIMER_LEFT_34_SEQUENCE=GGATTCCATGGGAAAGCAAG
PRIMER_RIGHT_34_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_34=478,20
PRIMER_RIGHT_34=1372,20
PRIMER_LEFT_34_TM=61.706
PRIMER_RIGHT_34_TM=61.335
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=895
PRIMER_PAIR_35_PENALTY=3.121055
PRIMER_LEFT_35_PENALTY=1.785955
PRIMER_RIGHT_35_PENALTY=1.335100
PRIMER_LEFT_35_SEQUENCE=TTCCATGGGAAAGCAAGTAA
PRIMER_RIGHT_35_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_35=481,20
PRIMER_RIGHT_35=1372,20
PRIMER_LEFT_35_TM=58.214
PRIMER_RIGHT_35_TM=61.335
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=5.7000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=892
PRIMER_PAIR_36_PENALTY=3.146885
PRIMER_LEFT_36_PENALTY=1.811785
PRIMER_RIGHT_36_PENALTY=1.335100
PRIMER_LEFT_36_SEQUENCE=ATGCTCAGGATTCAAACGAAC
PRIMER_RIGHT_36_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_36=382,21
PRIMER_RIGHT_36=1372,20
PRIMER_LEFT_36_TM=59.188
PRIMER_RIGHT_36_TM=61.335
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.4000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=991
PRIMER_PAIR_37_PENALTY=3.151721
PRIMER_LEFT_37_PENALTY=1.015638
PRIMER_RIGHT_37_PENALTY=2.136083
PRIMER_LEFT_37_SEQUENCE=GATTCCATGGGAAAGCAAGT
PRIMER_RIGHT_37_SEQUENCE=GTATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_37=479,20
PRIMER_RIGHT_37=1475,22
PRIMER_LEFT_37_TM=58.984
PRIMER_RIGHT_37_TM=59.864
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=8.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=997
PRIMER_PAIR_38_PENALTY=3.263153
PRIMER_LEFT_38_PENALTY=1.015638
PRIMER_RIGHT_38_PENALTY=2.247514
PRIMER_LEFT_38_SEQUENCE=GATTCCATGGGAAAGCAAGT
PRIMER_RIGHT_38_SEQUENCE=CGAGGAGATGATGAGGTAAGG
PRIMER_LEFT_38=479,20
PRIMER_RIGHT_38=1368,21
PRIMER_LEFT_38_TM=58.984
PRIMER_RIGHT_38_TM=58.752
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=7.5000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=890
PRIMER_PAIR_39_PENALTY=3.264915
PRIMER_LEFT_39_PENALTY=3.042133
PRIMER_RIGHT_39_PENALTY=0.222782
PRIMER_LEFT_39_SEQUENCE=TCTATACCCGTTGGATGTGAA
PRIMER_RIGHT_39_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_39=412,21
PRIMER_RIGHT_39=1371,20
PRIMER_LEFT_39_TM=57.958
PRIMER_RIGHT_39_TM=60.223
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.7000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=960
PRIMER_PAIR_40_PENALTY=3.268929
PRIMER_LEFT_40_PENALTY=2.312177
PRIMER_RIGHT_40_PENALTY=0.956752
PRIMER_LEFT_40_SEQUENCE=GATTCCATGGGAAAGCAAGTAA
PRIMER_RIGHT_40_SEQUENCE=ATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_40=479,22
PRIMER_RIGHT_40=1473,20
PRIMER_LEFT_40_TM=60.312
PRIMER_RIGHT_40_TM=59.043
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.7000
PRIMER_RIGHT_40_END_STABILITY=8.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=995
PRIMER_PAIR_41_PENALTY=3.288947
PRIMER_LEFT_41_PENALTY=1.376703
PRIMER_RIGHT_41_PENALTY=1.912245
PRIMER_LEFT_41_SEQUENCE=CCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_41_SEQUENCE=TATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_41=483,20
PRIMER_RIGHT_41=1474,21
PRIMER_LEFT_41_TM=58.623
PRIMER_RIGHT_41_TM=59.088
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.0000
PRIMER_RIGHT_41_END_STABILITY=8.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=992
PRIMER_PAIR_42_PENALTY=3.343686
PRIMER_LEFT_42_PENALTY=0.201241
PRIMER_RIGHT_42_PENALTY=3.142445
PRIMER_LEFT_42_SEQUENCE=CATGCTCAGGATTCAAACGA
PRIMER_RIGHT_42_SEQUENCE=TCGTCGAGGAGATGATGAGGT
PRIMER_LEFT_42=381,20
PRIMER_RIGHT_42=1372,21
PRIMER_LEFT_42_TM=59.799
PRIMER_RIGHT_42_TM=62.142
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=992
PRIMER_PAIR_43_PENALTY=3.386899
PRIMER_LEFT_43_PENALTY=3.164117
PRIMER_RIGHT_43_PENALTY=0.222782
PRIMER_LEFT_43_SEQUENCE=CATCTATACCCGTTGGATGTG
PRIMER_RIGHT_43_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_43=410,21
PRIMER_RIGHT_43=1371,20
PRIMER_LEFT_43_TM=57.836
PRIMER_RIGHT_43_TM=60.223
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=962
PRIMER_PAIR_44_PENALTY=3.397414
PRIMER_LEFT_44_PENALTY=1.149900
PRIMER_RIGHT_44_PENALTY=2.247514
PRIMER_LEFT_44_SEQUENCE=GCTCAGGATTCAAACGAACA
PRIMER_RIGHT_44_SEQUENCE=CGAGGAGATGATGAGGTAAGG
PRIMER_LEFT_44=384,20
PRIMER_RIGHT_44=1368,21
PRIMER_LEFT_44_TM=58.850
PRIMER_RIGHT_44_TM=58.752
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.7000
PRIMER_RIGHT_44_END_STABILITY=7.5000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=985
PRIMER_PAIR_45_PENALTY=3.397414
PRIMER_LEFT_45_PENALTY=1.149900
PRIMER_RIGHT_45_PENALTY=2.247514
PRIMER_LEFT_45_SEQUENCE=TGCTCAGGATTCAAACGAAC
PRIMER_RIGHT_45_SEQUENCE=CGAGGAGATGATGAGGTAAGG
PRIMER_LEFT_45=383,20
PRIMER_RIGHT_45=1368,21
PRIMER_LEFT_45_TM=58.850
PRIMER_RIGHT_45_TM=58.752
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.4000
PRIMER_RIGHT_45_END_STABILITY=7.5000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=986
PRIMER_PAIR_46_PENALTY=3.399341
PRIMER_LEFT_46_PENALTY=1.487097
PRIMER_RIGHT_46_PENALTY=1.912245
PRIMER_LEFT_46_SEQUENCE=TCCATGGGAAAGCAAGTAACA
PRIMER_RIGHT_46_SEQUENCE=TATTCGGATGCAAAGTTAGGG
PRIMER_LEFT_46=482,21
PRIMER_RIGHT_46=1474,21
PRIMER_LEFT_46_TM=60.487
PRIMER_RIGHT_46_TM=59.088
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.0000
PRIMER_RIGHT_46_END_STABILITY=8.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=993
PRIMER_PAIR_47_PENALTY=3.416999
PRIMER_LEFT_47_PENALTY=1.015638
PRIMER_RIGHT_47_PENALTY=2.401361
PRIMER_LEFT_47_SEQUENCE=GATTCCATGGGAAAGCAAGT
PRIMER_RIGHT_47_SEQUENCE=CGAGGAGATGATGAGGTAAGGT
PRIMER_LEFT_47=479,20
PRIMER_RIGHT_47=1368,22
PRIMER_LEFT_47_TM=58.984
PRIMER_RIGHT_47_TM=59.599
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=890
PRIMER_PAIR_48_PENALTY=3.437082
PRIMER_LEFT_48_PENALTY=3.214301
PRIMER_RIGHT_48_PENALTY=0.222782
PRIMER_LEFT_48_SEQUENCE=CGAACAAACGAACATCTATACCC
PRIMER_RIGHT_48_SEQUENCE=CGTCGAGGAGATGATGAGGT
PRIMER_LEFT_48=398,23
PRIMER_RIGHT_48=1371,20
PRIMER_LEFT_48_TM=59.786
PRIMER_RIGHT_48_TM=60.223
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.4000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=974
PRIMER_PAIR_49_PENALTY=3.470960
PRIMER_LEFT_49_PENALTY=2.135860
PRIMER_RIGHT_49_PENALTY=1.335100
PRIMER_LEFT_49_SEQUENCE=TACCCGTTGGATGTGAATCT
PRIMER_RIGHT_49_SEQUENCE=TCGTCGAGGAGATGATGAGG
PRIMER_LEFT_49=416,20
PRIMER_RIGHT_49=1372,20
PRIMER_LEFT_49_TM=57.864
PRIMER_RIGHT_49_TM=61.335
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.6000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=957
=
SEQUENCE_ID=MAGIC.338_3_16328577_16328585_no-0_ler-0_765027_14531_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTNACCNNTTNNNTTTTANCTATTTTCAANCCAACNANAAACTTATGTTANNNNNNNNNNNNNNNNNATTGGGCNGANGCCCANAATCNNNATAANTGAAGAAGNCGAGCGCTGCNNNNNNNNNNNNNNNNNNNAGTGTCAGTGNNAGTGAACAGTCAGNTTNTAGGGNNNNNNNNNTTTGGTCGTCCTTAACTTNANGCTTTGANAGTTTGGTTGNGNANTTCAATGGGGTATGAGANGTTNACTTGGGTNATTNAGAATTTCNNATNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCGTNATNGGTGGCTGCAAATGGTNAGNNGAAGACAANTTTNTTATATCTCNNNNNNNNNNNTTTNNNNNNNNNNNNNGGTTTANGAGNACATNNNTCCNNNNNNNNNANGGNNTTTTTGCTTGCAGGNTTCTTAAGGGATATCANNANGCTAANTATTTGTCTCTGNNTCTGNTGGTTGCTACTTCAAAAACATTGCCTNGTGGATGGAGAAGANACACACGATTTNGCCTAACTGTTGTAAATCAACTTTCCNATGAACTNTCCCAACAACGAGGTGANTTCTATAAGGCTTTTGGACAAAGNAAANTTCCTATTTTTGTGATTTNTATTTNTGTCTGCTTGTGTTTGTTTTGAGTTAGATCAGTTCTATCTTTTNTTTGTCTANAGAAANGGAAANATGGTTTGNNNAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTACCAAACTTANTGCNAAGAAAGGTGGATTTCTTGTNAACANTGAAGTCAAGATTGTTGTGGAGGTTGANGTTCTTCAAGTTNTTGGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAANGATAANGCTAAANGATGATGGTGNTTCCGTAAAANAAAGCANTGATGTCAATGGGTTTCNNGTTCTTCCTTCNCAGGTAACAAGTTCATAAAGATTNAAACTTTTNAGNTNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9016129_9016257_po-0_ler-0_43209_14015_TRUE
SEQUENCE_TARGET=950,228
SEQUENCE_TEMPLATE=AATGAAAAAAGTGGAAAAAAGATTGATGCTAATTCGTGGGACATGGAATTTGTGGAAGACCTTCCCCAACAAAAGAATGGGTATGTAACTATGCTTGAACTTGCTATGATNCATGATCATGTGAAAGGAACTATCTTGTTAAAGAGATAACCTCTGTAGAATCAGATACTTGTCTTCTGATGTNGGAATATGGNTTTTTAAGACAAAGGATAGATCTNNTTTGAGATTGATTAGNAGAAATTCACCTGATATNAATNTTGATNATGGAATCAACNAGGGNNTAACTTAACTNNNNNNANNNTCTTTAGCACTCTGGTTAAGCTCTCTGCTAACTNTNTNTTNTTTCTTTGNNGNAANCTTTTATATTNTCAGGTATGACTGTGGAATGTTTATGCTTAAGTACATCGATTTTTTCAGNAGAGGCCTGGGGCTATGTTTCAGCCAGGTAAGCTATCAGAATCCAAATCTCTNAATGCCTCAAGAAACNGTAGTTGAGAATNAAAACTGGTTGTCTTGAANCAATATGGAATTAGTATATCCCCCTAGCAGTTTATGTAACTTGTAGGATTTAGGCATTTAGCGTAATAACATTGTATTACTTTGCAGGAACACATGCCATACTTCCGACTCAGAACAGCTAAAGAGATTCTGAGNCTACGAGCTGATTGAAGTTGCTTGTGCTAATGTTTTTTCGGCTATTAGGAATACTATTTTTGGTTGCCATTGTTGATTTGTTCAAACTTTAAATCCCACTCCTTTTAGGCAGTGAAGCACTCGGCTTTAAAAGCAGAGGTAAANACTGAGTCAAGNCCAGAGCTCTTAGCTCGTTCNGTTATTGTNNTTTTNNNNNNCTTCTTGTTTTNAAATGTAGATGAAGCGTTTTGGATCTCTATGTTGGTTAGTCTAGCTGAAGATATATGAGATGTTGAAAACCTTATTTATTTCTCTCCGGTCNTGACCAGTGCGAANGCANTTTAAAGTTTCCATCNAGTATNGGTTCNATATANNNNNNNNNNNTCAATAGCCAAAANTCCATGAATAANCTCTTGAAAGCAAATGTTTTCGATNTACAAATGTTCTGCACANTTTCCCCAATACATACACGAATATGTTACAAATATACAACAANCTGCTGTNNGTGAGTACAAATGTTGACAAGGACACAGAAAAAACAAAATCCCAAATGTAACATTGACATATTAAAACCCACTCCCAAAAACAAAAACAAATTCCCCAATTCATANAACACNNGAGGAAGGAAGACTTGTGTGTATCTNCTAACAAAAACTGACTGGAANGCAAACTAACTTCCACTGCCTTTCCTAGNGCGGAACTCTCTTCNGGCTAAGTATCTCCTGTTCTTTCNGACGGTGTTGGAACCGAGCAAGACGGGCTTGAAGACTTACAAGACCAGCTGCTTTACGGGACAACACAAAGCTCAGCTGTGTAACATAGTTGTCAATCATGCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAATAATGTTAAAACAATCACAAGACATATGAGNNNTNACCTCGNGAACTATTTCCATTGTATCCTCGATCTCTTTTCTGTGTGCTGTAATTAAGGTCTCTTCTTCCTGCCAGAGNTTAAACAAAATAATTACCATATTACAGGTTTTNTAAGGTTATTCGTGAGCGATATTCATAAAATTCCCTCACGCTCATAGCATAATTTAGAAGTATAGAAGGGTCAAATGGCTCTTTACCTCAAGCAATGCATCAATGTTTTCATCAAGAGAAGGCTCCGTTGTTTCATACTGCCTTGAAGATGNATCNCTTGTGTTCTGACTGCTAGCCATATTTTGTTGTCTAGAATTTGTCATGGCAGGGACATCAGTCTTGAGAATAAGATGAGTGAATCCTNTGACTAGTGGAGCCGCCAAAAGAAGATTTTAAAATGTTAAGCATAAATCATATTCCTTAAATGTTAAGTGAATCCTTTGACTAGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.546217
PRIMER_LEFT_0_PENALTY=0.356982
PRIMER_RIGHT_0_PENALTY=0.189235
PRIMER_LEFT_0_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_0_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_0=714,20
PRIMER_RIGHT_0=1224,20
PRIMER_LEFT_0_TM=60.357
PRIMER_RIGHT_0_TM=59.811
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=1.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.2000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=511
PRIMER_PAIR_1_PENALTY=0.569543
PRIMER_LEFT_1_PENALTY=0.327117
PRIMER_RIGHT_1_PENALTY=0.242426
PRIMER_LEFT_1_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_1_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_1=611,20
PRIMER_RIGHT_1=1433,20
PRIMER_LEFT_1_TM=59.673
PRIMER_RIGHT_1_TM=59.758
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=823
PRIMER_PAIR_2_PENALTY=0.599409
PRIMER_LEFT_2_PENALTY=0.356982
PRIMER_RIGHT_2_PENALTY=0.242426
PRIMER_LEFT_2_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_2_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_2=714,20
PRIMER_RIGHT_2=1433,20
PRIMER_LEFT_2_TM=60.357
PRIMER_RIGHT_2_TM=59.758
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=720
PRIMER_PAIR_3_PENALTY=0.738374
PRIMER_LEFT_3_PENALTY=0.549139
PRIMER_RIGHT_3_PENALTY=0.189235
PRIMER_LEFT_3_SEQUENCE=TGCAGGAACACATGCCATAC
PRIMER_RIGHT_3_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_3=601,20
PRIMER_RIGHT_3=1224,20
PRIMER_LEFT_3_TM=60.549
PRIMER_RIGHT_3_TM=59.811
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=624
PRIMER_PAIR_4_PENALTY=0.791565
PRIMER_LEFT_4_PENALTY=0.549139
PRIMER_RIGHT_4_PENALTY=0.242426
PRIMER_LEFT_4_SEQUENCE=TGCAGGAACACATGCCATAC
PRIMER_RIGHT_4_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_4=601,20
PRIMER_RIGHT_4=1433,20
PRIMER_LEFT_4_TM=60.549
PRIMER_RIGHT_4_TM=59.758
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=833
PRIMER_PAIR_5_PENALTY=0.826983
PRIMER_LEFT_5_PENALTY=0.356982
PRIMER_RIGHT_5_PENALTY=0.470001
PRIMER_LEFT_5_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_5_SEQUENCE=TCTGGCAGGAAGAAGAGACC
PRIMER_LEFT_5=714,20
PRIMER_RIGHT_5=1635,20
PRIMER_LEFT_5_TM=60.357
PRIMER_RIGHT_5_TM=59.530
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=922
PRIMER_PAIR_6_PENALTY=1.019339
PRIMER_LEFT_6_PENALTY=0.327117
PRIMER_RIGHT_6_PENALTY=0.692222
PRIMER_LEFT_6_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_6_SEQUENCE=TGTTTTTGGGAGTGGGTTTT
PRIMER_LEFT_6=611,20
PRIMER_RIGHT_6=1220,20
PRIMER_LEFT_6_TM=59.673
PRIMER_RIGHT_6_TM=59.308
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=1.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=610
PRIMER_PAIR_7_PENALTY=1.019339
PRIMER_LEFT_7_PENALTY=0.327117
PRIMER_RIGHT_7_PENALTY=0.692222
PRIMER_LEFT_7_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_7_SEQUENCE=TTGTTTTTGGGAGTGGGTTT
PRIMER_LEFT_7=611,20
PRIMER_RIGHT_7=1221,20
PRIMER_LEFT_7_TM=59.673
PRIMER_RIGHT_7_TM=59.308
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=1.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=611
PRIMER_PAIR_8_PENALTY=1.019339
PRIMER_LEFT_8_PENALTY=0.327117
PRIMER_RIGHT_8_PENALTY=0.692222
PRIMER_LEFT_8_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_8_SEQUENCE=TTTGTTTTTGGGAGTGGGTT
PRIMER_LEFT_8=611,20
PRIMER_RIGHT_8=1222,20
PRIMER_LEFT_8_TM=59.673
PRIMER_RIGHT_8_TM=59.308
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=1.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=612
PRIMER_PAIR_9_PENALTY=1.019339
PRIMER_LEFT_9_PENALTY=0.327117
PRIMER_RIGHT_9_PENALTY=0.692222
PRIMER_LEFT_9_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_9_SEQUENCE=TTTTGTTTTTGGGAGTGGGT
PRIMER_LEFT_9=611,20
PRIMER_RIGHT_9=1223,20
PRIMER_LEFT_9_TM=59.673
PRIMER_RIGHT_9_TM=59.308
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=1.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=613
PRIMER_PAIR_10_PENALTY=1.049205
PRIMER_LEFT_10_PENALTY=0.356982
PRIMER_RIGHT_10_PENALTY=0.692222
PRIMER_LEFT_10_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_10_SEQUENCE=TGTTTTTGGGAGTGGGTTTT
PRIMER_LEFT_10=714,20
PRIMER_RIGHT_10=1220,20
PRIMER_LEFT_10_TM=60.357
PRIMER_RIGHT_10_TM=59.308
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=1.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=507
PRIMER_PAIR_11_PENALTY=1.049205
PRIMER_LEFT_11_PENALTY=0.356982
PRIMER_RIGHT_11_PENALTY=0.692222
PRIMER_LEFT_11_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_11_SEQUENCE=TTGTTTTTGGGAGTGGGTTT
PRIMER_LEFT_11=714,20
PRIMER_RIGHT_11=1221,20
PRIMER_LEFT_11_TM=60.357
PRIMER_RIGHT_11_TM=59.308
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=1.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.2000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=508
PRIMER_PAIR_12_PENALTY=1.049205
PRIMER_LEFT_12_PENALTY=0.356982
PRIMER_RIGHT_12_PENALTY=0.692222
PRIMER_LEFT_12_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_12_SEQUENCE=TTTGTTTTTGGGAGTGGGTT
PRIMER_LEFT_12=714,20
PRIMER_RIGHT_12=1222,20
PRIMER_LEFT_12_TM=60.357
PRIMER_RIGHT_12_TM=59.308
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=1.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=509
PRIMER_PAIR_13_PENALTY=1.049205
PRIMER_LEFT_13_PENALTY=0.356982
PRIMER_RIGHT_13_PENALTY=0.692222
PRIMER_LEFT_13_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_13_SEQUENCE=TTTTGTTTTTGGGAGTGGGT
PRIMER_LEFT_13=714,20
PRIMER_RIGHT_13=1223,20
PRIMER_LEFT_13_TM=60.357
PRIMER_RIGHT_13_TM=59.308
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=1.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.2000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=510
PRIMER_PAIR_14_PENALTY=1.170831
PRIMER_LEFT_14_PENALTY=0.327117
PRIMER_RIGHT_14_PENALTY=0.843714
PRIMER_LEFT_14_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_14_SEQUENCE=GCTGAGCTTTGTGTTGTCCC
PRIMER_LEFT_14=611,20
PRIMER_RIGHT_14=1442,20
PRIMER_LEFT_14_TM=59.673
PRIMER_RIGHT_14_TM=60.844
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=832
PRIMER_PAIR_15_PENALTY=1.200696
PRIMER_LEFT_15_PENALTY=0.356982
PRIMER_RIGHT_15_PENALTY=0.843714
PRIMER_LEFT_15_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_15_SEQUENCE=GCTGAGCTTTGTGTTGTCCC
PRIMER_LEFT_15=714,20
PRIMER_RIGHT_15=1442,20
PRIMER_LEFT_15_TM=60.357
PRIMER_RIGHT_15_TM=60.844
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.2000
PRIMER_RIGHT_15_END_STABILITY=9.1000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=729
PRIMER_PAIR_16_PENALTY=1.203947
PRIMER_LEFT_16_PENALTY=1.014712
PRIMER_RIGHT_16_PENALTY=0.189235
PRIMER_LEFT_16_SEQUENCE=GCAGGAACACATGCCATACTT
PRIMER_RIGHT_16_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_16=602,21
PRIMER_RIGHT_16=1224,20
PRIMER_LEFT_16_TM=60.015
PRIMER_RIGHT_16_TM=59.811
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=1.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=623
PRIMER_PAIR_17_PENALTY=1.241361
PRIMER_LEFT_17_PENALTY=0.549139
PRIMER_RIGHT_17_PENALTY=0.692222
PRIMER_LEFT_17_SEQUENCE=TGCAGGAACACATGCCATAC
PRIMER_RIGHT_17_SEQUENCE=TGTTTTTGGGAGTGGGTTTT
PRIMER_LEFT_17=601,20
PRIMER_RIGHT_17=1220,20
PRIMER_LEFT_17_TM=60.549
PRIMER_RIGHT_17_TM=59.308
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=1.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=5.6000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=620
PRIMER_PAIR_18_PENALTY=1.241361
PRIMER_LEFT_18_PENALTY=0.549139
PRIMER_RIGHT_18_PENALTY=0.692222
PRIMER_LEFT_18_SEQUENCE=TGCAGGAACACATGCCATAC
PRIMER_RIGHT_18_SEQUENCE=TTGTTTTTGGGAGTGGGTTT
PRIMER_LEFT_18=601,20
PRIMER_RIGHT_18=1221,20
PRIMER_LEFT_18_TM=60.549
PRIMER_RIGHT_18_TM=59.308
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=1.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=5.6000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=621
PRIMER_PAIR_19_PENALTY=1.241361
PRIMER_LEFT_19_PENALTY=0.549139
PRIMER_RIGHT_19_PENALTY=0.692222
PRIMER_LEFT_19_SEQUENCE=TGCAGGAACACATGCCATAC
PRIMER_RIGHT_19_SEQUENCE=TTTTGTTTTTGGGAGTGGGT
PRIMER_LEFT_19=601,20
PRIMER_RIGHT_19=1223,20
PRIMER_LEFT_19_TM=60.549
PRIMER_RIGHT_19_TM=59.308
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=1.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=623
PRIMER_PAIR_20_PENALTY=1.257138
PRIMER_LEFT_20_PENALTY=1.014712
PRIMER_RIGHT_20_PENALTY=0.242426
PRIMER_LEFT_20_SEQUENCE=GCAGGAACACATGCCATACTT
PRIMER_RIGHT_20_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_20=602,21
PRIMER_RIGHT_20=1433,20
PRIMER_LEFT_20_TM=60.015
PRIMER_RIGHT_20_TM=59.758
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=5.7000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=832
PRIMER_PAIR_21_PENALTY=1.296687
PRIMER_LEFT_21_PENALTY=1.107452
PRIMER_RIGHT_21_PENALTY=0.189235
PRIMER_LEFT_21_SEQUENCE=ACTTTGCAGGAACACATGCC
PRIMER_RIGHT_21_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_21=597,20
PRIMER_RIGHT_21=1224,20
PRIMER_LEFT_21_TM=61.107
PRIMER_RIGHT_21_TM=59.811
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=1.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=628
PRIMER_PAIR_22_PENALTY=1.315428
PRIMER_LEFT_22_PENALTY=1.126193
PRIMER_RIGHT_22_PENALTY=0.189235
PRIMER_LEFT_22_SEQUENCE=AACACATGCCATACTTCCGAC
PRIMER_RIGHT_22_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_22=607,21
PRIMER_RIGHT_22=1224,20
PRIMER_LEFT_22_TM=59.874
PRIMER_RIGHT_22_TM=59.811
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=1.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=9.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=618
PRIMER_PAIR_23_PENALTY=1.349879
PRIMER_LEFT_23_PENALTY=1.107452
PRIMER_RIGHT_23_PENALTY=0.242426
PRIMER_LEFT_23_SEQUENCE=ACTTTGCAGGAACACATGCC
PRIMER_RIGHT_23_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_23=597,20
PRIMER_RIGHT_23=1433,20
PRIMER_LEFT_23_TM=61.107
PRIMER_RIGHT_23_TM=59.758
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.6000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=7.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=837
PRIMER_PAIR_24_PENALTY=1.368620
PRIMER_LEFT_24_PENALTY=1.126193
PRIMER_RIGHT_24_PENALTY=0.242426
PRIMER_LEFT_24_SEQUENCE=AACACATGCCATACTTCCGAC
PRIMER_RIGHT_24_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_24=607,21
PRIMER_RIGHT_24=1433,20
PRIMER_LEFT_24_TM=59.874
PRIMER_RIGHT_24_TM=59.758
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.6000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=827
PRIMER_PAIR_25_PENALTY=1.392853
PRIMER_LEFT_25_PENALTY=0.549139
PRIMER_RIGHT_25_PENALTY=0.843714
PRIMER_LEFT_25_SEQUENCE=TGCAGGAACACATGCCATAC
PRIMER_RIGHT_25_SEQUENCE=GCTGAGCTTTGTGTTGTCCC
PRIMER_LEFT_25=601,20
PRIMER_RIGHT_25=1442,20
PRIMER_LEFT_25_TM=60.549
PRIMER_RIGHT_25_TM=60.844
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=5.6000
PRIMER_RIGHT_25_END_STABILITY=9.1000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=842
PRIMER_PAIR_26_PENALTY=1.488207
PRIMER_LEFT_26_PENALTY=0.327117
PRIMER_RIGHT_26_PENALTY=1.161090
PRIMER_LEFT_26_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_26_SEQUENCE=GAGCTTTGTGTTGTCCCGTAA
PRIMER_LEFT_26=611,20
PRIMER_RIGHT_26=1439,21
PRIMER_LEFT_26_TM=59.673
PRIMER_RIGHT_26_TM=60.161
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=7.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=829
PRIMER_PAIR_27_PENALTY=1.517652
PRIMER_LEFT_27_PENALTY=1.047652
PRIMER_RIGHT_27_PENALTY=0.470001
PRIMER_LEFT_27_SEQUENCE=AAATCCCACTCCTTTTAGGCA
PRIMER_RIGHT_27_SEQUENCE=TCTGGCAGGAAGAAGAGACC
PRIMER_LEFT_27=744,21
PRIMER_RIGHT_27=1635,20
PRIMER_LEFT_27_TM=59.952
PRIMER_RIGHT_27_TM=59.530
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=892
PRIMER_PAIR_28_PENALTY=1.518073
PRIMER_LEFT_28_PENALTY=0.356982
PRIMER_RIGHT_28_PENALTY=1.161090
PRIMER_LEFT_28_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_28_SEQUENCE=GAGCTTTGTGTTGTCCCGTAA
PRIMER_LEFT_28=714,20
PRIMER_RIGHT_28=1439,21
PRIMER_LEFT_28_TM=60.357
PRIMER_RIGHT_28_TM=60.161
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.2000
PRIMER_RIGHT_28_END_STABILITY=7.7000
PRIMER_PAIR_28_COMPL_ANY=2.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=726
PRIMER_PAIR_29_PENALTY=1.539837
PRIMER_LEFT_29_PENALTY=1.350602
PRIMER_RIGHT_29_PENALTY=0.189235
PRIMER_LEFT_29_SEQUENCE=TCCTTTTAGGCAGTGAAGCA
PRIMER_RIGHT_29_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_29=753,20
PRIMER_RIGHT_29=1224,20
PRIMER_LEFT_29_TM=58.649
PRIMER_RIGHT_29_TM=59.811
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=1.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=9.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=472
PRIMER_PAIR_30_PENALTY=1.548891
PRIMER_LEFT_30_PENALTY=1.359656
PRIMER_RIGHT_30_PENALTY=0.189235
PRIMER_LEFT_30_SEQUENCE=CTCCTTTTAGGCAGTGAAGCA
PRIMER_RIGHT_30_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_30=752,21
PRIMER_RIGHT_30=1224,20
PRIMER_LEFT_30_TM=59.640
PRIMER_RIGHT_30_TM=59.811
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=1.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=473
PRIMER_PAIR_31_PENALTY=1.590469
PRIMER_LEFT_31_PENALTY=1.401234
PRIMER_RIGHT_31_PENALTY=0.189235
PRIMER_LEFT_31_SEQUENCE=GGGCTATGTTTCAGCCAGGT
PRIMER_RIGHT_31_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_31=427,20
PRIMER_RIGHT_31=1224,20
PRIMER_LEFT_31_TM=61.401
PRIMER_RIGHT_31_TM=59.811
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=1.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=798
PRIMER_PAIR_32_PENALTY=1.593029
PRIMER_LEFT_32_PENALTY=1.350602
PRIMER_RIGHT_32_PENALTY=0.242426
PRIMER_LEFT_32_SEQUENCE=TCCTTTTAGGCAGTGAAGCA
PRIMER_RIGHT_32_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_32=753,20
PRIMER_RIGHT_32=1433,20
PRIMER_LEFT_32_TM=58.649
PRIMER_RIGHT_32_TM=59.758
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=681
PRIMER_PAIR_33_PENALTY=1.602083
PRIMER_LEFT_33_PENALTY=1.359656
PRIMER_RIGHT_33_PENALTY=0.242426
PRIMER_LEFT_33_SEQUENCE=CTCCTTTTAGGCAGTGAAGCA
PRIMER_RIGHT_33_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_33=752,21
PRIMER_RIGHT_33=1433,20
PRIMER_LEFT_33_TM=59.640
PRIMER_RIGHT_33_TM=59.758
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=682
PRIMER_PAIR_34_PENALTY=1.644972
PRIMER_LEFT_34_PENALTY=1.402545
PRIMER_RIGHT_34_PENALTY=0.242426
PRIMER_LEFT_34_SEQUENCE=CACATGCCATACTTCCGACT
PRIMER_RIGHT_34_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_34=609,20
PRIMER_RIGHT_34=1433,20
PRIMER_LEFT_34_TM=58.597
PRIMER_RIGHT_34_TM=59.758
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=825
PRIMER_PAIR_35_PENALTY=1.651751
PRIMER_LEFT_35_PENALTY=0.327117
PRIMER_RIGHT_35_PENALTY=1.324634
PRIMER_LEFT_35_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_35_SEQUENCE=GCTTTGTGTTGTCCCGTAAA
PRIMER_LEFT_35=611,20
PRIMER_RIGHT_35=1437,20
PRIMER_LEFT_35_TM=59.673
PRIMER_RIGHT_35_TM=58.675
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=6.0000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=827
PRIMER_PAIR_36_PENALTY=1.664391
PRIMER_LEFT_36_PENALTY=0.327117
PRIMER_RIGHT_36_PENALTY=1.337274
PRIMER_LEFT_36_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_36_SEQUENCE=GCTTTGTGTTGTCCCGTAAAG
PRIMER_LEFT_36=611,20
PRIMER_RIGHT_36=1437,21
PRIMER_LEFT_36_TM=59.673
PRIMER_RIGHT_36_TM=59.663
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=6.3000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=827
PRIMER_PAIR_37_PENALTY=1.664391
PRIMER_LEFT_37_PENALTY=0.327117
PRIMER_RIGHT_37_PENALTY=1.337274
PRIMER_LEFT_37_SEQUENCE=CATGCCATACTTCCGACTCA
PRIMER_RIGHT_37_SEQUENCE=AGCTTTGTGTTGTCCCGTAAA
PRIMER_LEFT_37=611,20
PRIMER_RIGHT_37=1438,21
PRIMER_LEFT_37_TM=59.673
PRIMER_RIGHT_37_TM=59.663
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=6.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=828
PRIMER_PAIR_38_PENALTY=1.681617
PRIMER_LEFT_38_PENALTY=0.356982
PRIMER_RIGHT_38_PENALTY=1.324634
PRIMER_LEFT_38_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_38_SEQUENCE=GCTTTGTGTTGTCCCGTAAA
PRIMER_LEFT_38=714,20
PRIMER_RIGHT_38=1437,20
PRIMER_LEFT_38_TM=60.357
PRIMER_RIGHT_38_TM=58.675
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.2000
PRIMER_RIGHT_38_END_STABILITY=6.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=724
PRIMER_PAIR_39_PENALTY=1.688721
PRIMER_LEFT_39_PENALTY=1.499486
PRIMER_RIGHT_39_PENALTY=0.189235
PRIMER_LEFT_39_SEQUENCE=TTAGGCAGTGAAGCACTCGG
PRIMER_RIGHT_39_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_39=758,20
PRIMER_RIGHT_39=1224,20
PRIMER_LEFT_39_TM=61.499
PRIMER_RIGHT_39_TM=59.811
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=1.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.9000
PRIMER_RIGHT_39_END_STABILITY=9.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=467
PRIMER_PAIR_40_PENALTY=1.694257
PRIMER_LEFT_40_PENALTY=0.356982
PRIMER_RIGHT_40_PENALTY=1.337274
PRIMER_LEFT_40_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_40_SEQUENCE=GCTTTGTGTTGTCCCGTAAAG
PRIMER_LEFT_40=714,20
PRIMER_RIGHT_40=1437,21
PRIMER_LEFT_40_TM=60.357
PRIMER_RIGHT_40_TM=59.663
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.2000
PRIMER_RIGHT_40_END_STABILITY=6.3000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=724
PRIMER_PAIR_41_PENALTY=1.694257
PRIMER_LEFT_41_PENALTY=0.356982
PRIMER_RIGHT_41_PENALTY=1.337274
PRIMER_LEFT_41_SEQUENCE=TGGTTGCCATTGTTGATTTG
PRIMER_RIGHT_41_SEQUENCE=AGCTTTGTGTTGTCCCGTAAA
PRIMER_LEFT_41=714,20
PRIMER_RIGHT_41=1438,21
PRIMER_LEFT_41_TM=60.357
PRIMER_RIGHT_41_TM=59.663
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.2000
PRIMER_RIGHT_41_END_STABILITY=6.0000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=725
PRIMER_PAIR_42_PENALTY=1.706934
PRIMER_LEFT_42_PENALTY=1.014712
PRIMER_RIGHT_42_PENALTY=0.692222
PRIMER_LEFT_42_SEQUENCE=GCAGGAACACATGCCATACTT
PRIMER_RIGHT_42_SEQUENCE=TGTTTTTGGGAGTGGGTTTT
PRIMER_LEFT_42=602,21
PRIMER_RIGHT_42=1220,20
PRIMER_LEFT_42_TM=60.015
PRIMER_RIGHT_42_TM=59.308
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=1.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.7000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=619
PRIMER_PAIR_43_PENALTY=1.706934
PRIMER_LEFT_43_PENALTY=1.014712
PRIMER_RIGHT_43_PENALTY=0.692222
PRIMER_LEFT_43_SEQUENCE=GCAGGAACACATGCCATACTT
PRIMER_RIGHT_43_SEQUENCE=TTGTTTTTGGGAGTGGGTTT
PRIMER_LEFT_43=602,21
PRIMER_RIGHT_43=1221,20
PRIMER_LEFT_43_TM=60.015
PRIMER_RIGHT_43_TM=59.308
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=1.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=5.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=620
PRIMER_PAIR_44_PENALTY=1.706934
PRIMER_LEFT_44_PENALTY=1.014712
PRIMER_RIGHT_44_PENALTY=0.692222
PRIMER_LEFT_44_SEQUENCE=GCAGGAACACATGCCATACTT
PRIMER_RIGHT_44_SEQUENCE=TTTGTTTTTGGGAGTGGGTT
PRIMER_LEFT_44=602,21
PRIMER_RIGHT_44=1222,20
PRIMER_LEFT_44_TM=60.015
PRIMER_RIGHT_44_TM=59.308
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=1.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=5.7000
PRIMER_RIGHT_44_END_STABILITY=9.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=621
PRIMER_PAIR_45_PENALTY=1.706934
PRIMER_LEFT_45_PENALTY=1.014712
PRIMER_RIGHT_45_PENALTY=0.692222
PRIMER_LEFT_45_SEQUENCE=GCAGGAACACATGCCATACTT
PRIMER_RIGHT_45_SEQUENCE=TTTTGTTTTTGGGAGTGGGT
PRIMER_LEFT_45=602,21
PRIMER_RIGHT_45=1223,20
PRIMER_LEFT_45_TM=60.015
PRIMER_RIGHT_45_TM=59.308
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=1.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=5.7000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=622
PRIMER_PAIR_46_PENALTY=1.710229
PRIMER_LEFT_46_PENALTY=0.549139
PRIMER_RIGHT_46_PENALTY=1.161090
PRIMER_LEFT_46_SEQUENCE=TGCAGGAACACATGCCATAC
PRIMER_RIGHT_46_SEQUENCE=GAGCTTTGTGTTGTCCCGTAA
PRIMER_LEFT_46=601,20
PRIMER_RIGHT_46=1439,21
PRIMER_LEFT_46_TM=60.549
PRIMER_RIGHT_46_TM=60.161
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=5.6000
PRIMER_RIGHT_46_END_STABILITY=7.7000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=839
PRIMER_PAIR_47_PENALTY=1.739874
PRIMER_LEFT_47_PENALTY=1.047652
PRIMER_RIGHT_47_PENALTY=0.692222
PRIMER_LEFT_47_SEQUENCE=AAATCCCACTCCTTTTAGGCA
PRIMER_RIGHT_47_SEQUENCE=TTTTGTTTTTGGGAGTGGGT
PRIMER_LEFT_47=744,21
PRIMER_RIGHT_47=1223,20
PRIMER_LEFT_47_TM=59.952
PRIMER_RIGHT_47_TM=59.308
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=1.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=8.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=480
PRIMER_PAIR_48_PENALTY=1.741913
PRIMER_LEFT_48_PENALTY=1.499486
PRIMER_RIGHT_48_PENALTY=0.242426
PRIMER_LEFT_48_SEQUENCE=TTAGGCAGTGAAGCACTCGG
PRIMER_RIGHT_48_SEQUENCE=TGTGTTGTCCCGTAAAGCAG
PRIMER_LEFT_48=758,20
PRIMER_RIGHT_48=1433,20
PRIMER_LEFT_48_TM=61.499
PRIMER_RIGHT_48_TM=59.758
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.9000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=676
PRIMER_PAIR_49_PENALTY=1.745422
PRIMER_LEFT_49_PENALTY=1.556187
PRIMER_RIGHT_49_PENALTY=0.189235
PRIMER_LEFT_49_SEQUENCE=ACACATGCCATACTTCCGAC
PRIMER_RIGHT_49_SEQUENCE=TTTTTGTTTTTGGGAGTGGG
PRIMER_LEFT_49=608,20
PRIMER_RIGHT_49=1224,20
PRIMER_LEFT_49_TM=58.444
PRIMER_RIGHT_49_TM=59.811
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=1.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=617
=
SEQUENCE_ID=MAGIC.105_5_10064706_10065301_ler-0_zu-0_23916_22145_TRUE
SEQUENCE_TARGET=950,695
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNGTNGTTATACNCTGAAAAAGCNGGATATCCGGCTCCGTTACAGGGNTNANCCANTTGGTTATTCATTTACAACATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGCCANNNCTCACCTANTTTTTNATTACAACACANNATTNTAGTGNAAATNTNTTTTCNCCAGTTGTNAAATNGTTTACNACAAACCATNNNNTTANTCTTANGAGAANNCACNNCAGCACTCTCCCCAAAGNTCTNANAATCTCTCTTCTAAGTGCCATGTGGCATAAGNCAAATGTCANTTCNACNTCATNTGAGATNGAANGCNTCCTCTTCTTCANGAAANTTCNTNGGAGTTGNGTTCNATTCAGGNTANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14551935_14552195_ws-0_mt-0_16150_32202_TRUE
SEQUENCE_TARGET=950,360
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_8455500_8455596_edi-0_oy-0_52465_1829873_TRUE
SEQUENCE_TARGET=950,196
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATCAGATTNATCNATGNTGGATTGAGAAAAAGCCTCAAGCTTGGAANAACTACATNTAAATCATAACNTCNAGATATTTTAAGGCTTTCNATGTCNCAAAGAAAGTTATTTGGTGTCNAATCNATGAGTGAAAATTGAGCTTTTCACATAGAACAAAGAGNGATATGAAATAGTACAATTTTATGTTTTAAATAAAGNAGATAAAATGAAAGGTAAATTTTATAGGTTTAGCCAGCAGANAACAAATNAAATGAATGTTTTAAGTTTAGACACCNAGAACAAACTTAGGGTTCAAGTCTAAGCTTAGTCATATACTTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGAAGAGAGAGAGATATAGAGACACTGAAAGCAAAGACCAAAAAANNNNNNNNNNNNNNAGAGAGAGGAAAATGGAGAGCGACGAAGCAGCAGCAGTGTCTCCTCAAGCAACGACACCGAGCGGAGGAACCGGAGCTTCTGGGCCGAAGAAGAGAGGTCGGAAACCTAAAACCAAGGAAGATTCTCAGACGCCGTCGTCTCAGCAACAGAGCGATGTTAAAATGAAAGAAAGTGGGAAGAAAACGCAGCAGTCGCCGAGTGTTGACGAGAAGTACTCTCAGTGGAAAGGTCTCGTCCCCATTCTCTACGACTGGCTCGCTAACCATAACCTCGTCTGGCCTTCACTCTCTTGCAGGTCTNCCCCTTTCTCCTTTCCTCTCCTTCTAGGGTTTCGTTTCGTAATCGTTTCTTAGCTTTGAACATTCTCATGTTTGGAATGAATTTAGTAAAATCNNACACATACATTTTCTCGATTTCTGGGTTTAAGTGAGATTGTTGCGATTGTTCTAGTTAGGGTTTTGGATGTGGCTCTGTCTTCATACCTTGATATATCTGATGTTCTATTCATGAATTNTTACTATTGATTACCTTGTTGGTTACTAATGACTAAGAGGAATTTTCAGTTTCTCTGAGNGTTTATATCTGATGAAGTCTTTAGTTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_13971553_13971607_oy-0_hi-0_80143_11952_TRUE
SEQUENCE_TARGET=950,154
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_12771597_12771741_ct-1_no-0_9576_6247_TRUE
SEQUENCE_TARGET=950,244
SEQUENCE_TEMPLATE=AGGCGAGAGGGTTATGTTTCNGCTGCAANNAACCNNNNNTATTCCCCTCAANATGATTGCCANTANAAGTCTCTTAGAGCATCCTCAANGGGAGGATTTAAGATNTTTNTTAACTAAATAAATNATTAAAATTAANTCCAAAATAATGACTTTAGGTTAGANGCGCTCTTAGCNTAGAGGCGCTCTTAGGTAATTGCAATGGTNATNTGGCATTGTTTGATGTCTTTACNTNNCTNCTNTCTCTCTCTCTCTCTNNNNNNNNNNNNGTCTCCCTCNCACCGGAGATGGATTCTTCANTATCTTCTCCTCAATCACAAATCCTTTATTGATCANACTATTCANNNNNNNNNNNNTACACGCTGCTCCTCTTCTTTTCCTTAGATTCCATGGCTTCACNNGAGAAATNNNNTCCTTGATAGTTAAGAACTGGAGATCTCTGTTATATCGATGAAGATGGATTCATATTTGTGGTTAATCGGTTAAANGAACTGATCAAGTACTATGTTAACATAGATTGTTACTTTAATTNCTTTAGTAAAGATTTATACTGGTTTTGGATGTATACTTTGTGANTNAAGNGAGANAANNNANTTTACAACTACNGTANNTGATTGATTTTTTAAGAACTCCANCTTANTATATTATCATTGNCAGANGNGTATCAAGGANTTTTTTTACCCAGTTCAAAACGTAACAAATAAAATGTCTTTAATNNAAAAAAATCNTTTTTTTACAACTTCTCAAACNAAAANAAACGTCCTACAAAAGCATCCTACGATCCTTTATGTCNTGNAATCTCTTTATCACAATCTCATTTGTAATANTATTGAATACATCTTTCTCAATAAAACAAACTAAACAATCATTCAAAAATTNNTCTCCAATACAATTTCGTCGATGTGTCTTTACGATCNTCATGGCTGAAAAACATCTCTCAACAGTAGCANNAGCAACANGCAAAATCAAAACCAATTTCAAAAGCCTATAACCAAANGGATATNACAAANGTTTTCGTGTTTCCACCANCTTCTGANCAATGTCTCCAAGATTCTTTAAATTAGAAAATCTCTCATCATTTCGAANAGTATCAATGTAGATAGATAATTNTTNCTCAATAGATATGAACTCTCCTAAACTAAAGTNANCTTGGTAGAACTTAGATAACCTCACNAACTTTGAGTGATCAAACTCANAAAATGCATCAATGGGAGNTAAAGAAGCANCACAAATAAGCAATTCAGTGTTCACCTCATTAAAANGGTCGTCAAATTTCTGAAGNTNGAAATCCAAAACANAATATAAGCAATTGACCTTGTAGTGATGCATATTTGATACTCTTNTTTTGTTTCTTCGTCTCTTTGAGTCAACAAAATCTTCCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAAACNAANAAACTTTCANNATGAGAGAATCTCACCCATCATCCCTAAAACACTGAAGTTGTCTCTTAGTTGATTTCACCAATGANATNGCATTNAAAATATNNTGATCTCTTCTTTNGAGNNCCATAGACAAGTTCTNTGTGAGCNCTANAANGTGTATCATCATGTGNNNNNNNNNNAANNNNTCAAAGNTATGAAAGNANNTNAGGAGACTATGTGCTTGNCGTCTNNNNNNGTNATCTANGCCGTAATTCTCAAAGCATTCNAGNACCTTGATGATAGAANAGTACANCTNNNNNNNNNNNNNNNNNNNNNNNNNNTGAGAATTCNAACGAGTANNTNCTGNTCNTTGANGAGANANCTCTTGATTNAACCNTNTTNTAGTACTGANTTCANCNNNNATNNNNCCNNCTNNNNNNNNNNNNNNNNNNNNNNNTCGGATCAAATNTTTCCNCTTGCATGAAGCTCNAACNACATTTAACAACAAAGAAATCANATNNAAAAAGTCTCCAACATTNANATGNTTTTNTNCAACTNNNNNNACAACTAANTGAANNTNNNNNNNNNNNCAATGAACATAANATGNTGATGTGNTNTCCTTCAAAATTAATGANTNNNNNNNNNNNNNNNNNNNNTTCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.420442
PRIMER_LEFT_0_PENALTY=2.243575
PRIMER_RIGHT_0_PENALTY=1.176867
PRIMER_LEFT_0_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_0_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_0=880,22
PRIMER_RIGHT_0=1463,21
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=60.177
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=584
PRIMER_PAIR_1_PENALTY=3.870922
PRIMER_LEFT_1_PENALTY=2.243575
PRIMER_RIGHT_1_PENALTY=1.627348
PRIMER_LEFT_1_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_1_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_1=880,22
PRIMER_RIGHT_1=1461,21
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=59.373
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=582
PRIMER_PAIR_2_PENALTY=3.945515
PRIMER_LEFT_2_PENALTY=2.243575
PRIMER_RIGHT_2_PENALTY=1.701940
PRIMER_LEFT_2_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_2_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_2=880,22
PRIMER_RIGHT_2=1466,21
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=59.298
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=587
PRIMER_PAIR_3_PENALTY=3.973346
PRIMER_LEFT_3_PENALTY=2.243575
PRIMER_RIGHT_3_PENALTY=1.729772
PRIMER_LEFT_3_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_3_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_3=880,22
PRIMER_RIGHT_3=1463,20
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=58.270
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=584
PRIMER_PAIR_4_PENALTY=4.083366
PRIMER_LEFT_4_PENALTY=2.243575
PRIMER_RIGHT_4_PENALTY=1.839792
PRIMER_LEFT_4_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_4_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_4=880,22
PRIMER_RIGHT_4=1464,21
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=59.160
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=585
PRIMER_PAIR_5_PENALTY=4.310198
PRIMER_LEFT_5_PENALTY=2.243575
PRIMER_RIGHT_5_PENALTY=2.066624
PRIMER_LEFT_5_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_5_SEQUENCE=TTTAGGGATGATGGGTGAGA
PRIMER_LEFT_5=880,22
PRIMER_RIGHT_5=1460,20
PRIMER_LEFT_5_TM=60.244
PRIMER_RIGHT_5_TM=57.933
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=581
PRIMER_PAIR_6_PENALTY=4.353085
PRIMER_LEFT_6_PENALTY=2.243575
PRIMER_RIGHT_6_PENALTY=2.109510
PRIMER_LEFT_6_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_6_SEQUENCE=AGTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_6=880,22
PRIMER_RIGHT_6=1465,22
PRIMER_LEFT_6_TM=60.244
PRIMER_RIGHT_6_TM=60.110
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=586
PRIMER_PAIR_7_PENALTY=4.353085
PRIMER_LEFT_7_PENALTY=2.243575
PRIMER_RIGHT_7_PENALTY=2.109510
PRIMER_LEFT_7_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_7_SEQUENCE=CAGTGTTTTAGGGATGATGGGT
PRIMER_LEFT_7=880,22
PRIMER_RIGHT_7=1466,22
PRIMER_LEFT_7_TM=60.244
PRIMER_RIGHT_7_TM=60.110
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=45.455
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=587
PRIMER_PAIR_8_PENALTY=4.430523
PRIMER_LEFT_8_PENALTY=2.243575
PRIMER_RIGHT_8_PENALTY=2.186949
PRIMER_LEFT_8_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_8_SEQUENCE=GTTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_8=880,22
PRIMER_RIGHT_8=1462,22
PRIMER_LEFT_8_TM=60.244
PRIMER_RIGHT_8_TM=60.187
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=583
PRIMER_PAIR_9_PENALTY=4.577644
PRIMER_LEFT_9_PENALTY=2.243575
PRIMER_RIGHT_9_PENALTY=2.334070
PRIMER_LEFT_9_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_9_SEQUENCE=TTTAGGGATGATGGGTGAGATT
PRIMER_LEFT_9=880,22
PRIMER_RIGHT_9=1460,22
PRIMER_LEFT_9_TM=60.244
PRIMER_RIGHT_9_TM=59.666
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=40.909
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=581
PRIMER_PAIR_10_PENALTY=4.784661
PRIMER_LEFT_10_PENALTY=2.243575
PRIMER_RIGHT_10_PENALTY=2.541086
PRIMER_LEFT_10_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_10_SEQUENCE=TTTTAGGGATGATGGGTGAG
PRIMER_LEFT_10=880,22
PRIMER_RIGHT_10=1461,20
PRIMER_LEFT_10_TM=60.244
PRIMER_RIGHT_10_TM=57.459
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=582
PRIMER_PAIR_11_PENALTY=4.871363
PRIMER_LEFT_11_PENALTY=2.243575
PRIMER_RIGHT_11_PENALTY=2.627789
PRIMER_LEFT_11_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_11_SEQUENCE=GTTTTAGGGATGATGGGTGAG
PRIMER_LEFT_11=880,22
PRIMER_RIGHT_11=1462,21
PRIMER_LEFT_11_TM=60.244
PRIMER_RIGHT_11_TM=58.372
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=583
PRIMER_PAIR_12_PENALTY=4.923077
PRIMER_LEFT_12_PENALTY=2.243575
PRIMER_RIGHT_12_PENALTY=2.679503
PRIMER_LEFT_12_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_12_SEQUENCE=TTAGGGATGATGGGTGAGATT
PRIMER_LEFT_12=880,22
PRIMER_RIGHT_12=1459,21
PRIMER_LEFT_12_TM=60.244
PRIMER_RIGHT_12_TM=58.320
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=580
PRIMER_PAIR_13_PENALTY=4.927528
PRIMER_LEFT_13_PENALTY=3.750661
PRIMER_RIGHT_13_PENALTY=1.176867
PRIMER_LEFT_13_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_13_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_13=677,20
PRIMER_RIGHT_13=1463,21
PRIMER_LEFT_13_TM=56.249
PRIMER_RIGHT_13_TM=60.177
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=787
PRIMER_PAIR_14_PENALTY=4.933843
PRIMER_LEFT_14_PENALTY=3.756976
PRIMER_RIGHT_14_PENALTY=1.176867
PRIMER_LEFT_14_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_14_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_14=914,20
PRIMER_RIGHT_14=1463,21
PRIMER_LEFT_14_TM=56.243
PRIMER_RIGHT_14_TM=60.177
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.3000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=550
PRIMER_PAIR_15_PENALTY=4.943091
PRIMER_LEFT_15_PENALTY=2.243575
PRIMER_RIGHT_15_PENALTY=2.699517
PRIMER_LEFT_15_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_15_SEQUENCE=GTTTTAGGGATGATGGGTGA
PRIMER_LEFT_15=880,22
PRIMER_RIGHT_15=1462,20
PRIMER_LEFT_15_TM=60.244
PRIMER_RIGHT_15_TM=57.300
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=583
PRIMER_PAIR_16_PENALTY=4.990110
PRIMER_LEFT_16_PENALTY=3.813243
PRIMER_RIGHT_16_PENALTY=1.176867
PRIMER_LEFT_16_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_16_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_16=886,20
PRIMER_RIGHT_16=1463,21
PRIMER_LEFT_16_TM=56.187
PRIMER_RIGHT_16_TM=60.177
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=578
PRIMER_PAIR_17_PENALTY=5.211491
PRIMER_LEFT_17_PENALTY=2.243575
PRIMER_RIGHT_17_PENALTY=2.967916
PRIMER_LEFT_17_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_17_SEQUENCE=GTGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_17=880,22
PRIMER_RIGHT_17=1464,22
PRIMER_LEFT_17_TM=60.244
PRIMER_RIGHT_17_TM=60.968
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=585
PRIMER_PAIR_18_PENALTY=5.212823
PRIMER_LEFT_18_PENALTY=4.035956
PRIMER_RIGHT_18_PENALTY=1.176867
PRIMER_LEFT_18_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_18_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_18=752,24
PRIMER_RIGHT_18=1463,21
PRIMER_LEFT_18_TM=59.964
PRIMER_RIGHT_18_TM=60.177
PRIMER_LEFT_18_GC_PERCENT=41.667
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=712
PRIMER_PAIR_19_PENALTY=5.294586
PRIMER_LEFT_19_PENALTY=2.243575
PRIMER_RIGHT_19_PENALTY=3.051012
PRIMER_LEFT_19_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_19_SEQUENCE=TTCAGTGTTTTAGGGATGATGG
PRIMER_LEFT_19=880,22
PRIMER_RIGHT_19=1468,22
PRIMER_LEFT_19_TM=60.244
PRIMER_RIGHT_19_TM=58.949
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=589
PRIMER_PAIR_20_PENALTY=5.331478
PRIMER_LEFT_20_PENALTY=2.243575
PRIMER_RIGHT_20_PENALTY=3.087904
PRIMER_LEFT_20_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_20_SEQUENCE=TGTTTTAGGGATGATGGGTGAG
PRIMER_LEFT_20=880,22
PRIMER_RIGHT_20=1463,22
PRIMER_LEFT_20_TM=60.244
PRIMER_RIGHT_20_TM=61.088
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=45.455
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.6000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=584
PRIMER_PAIR_21_PENALTY=5.331478
PRIMER_LEFT_21_PENALTY=2.243575
PRIMER_RIGHT_21_PENALTY=3.087904
PRIMER_LEFT_21_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_21_SEQUENCE=TCAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_21=880,22
PRIMER_RIGHT_21=1467,22
PRIMER_LEFT_21_TM=60.244
PRIMER_RIGHT_21_TM=61.088
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=588
PRIMER_PAIR_22_PENALTY=5.364750
PRIMER_LEFT_22_PENALTY=2.243575
PRIMER_RIGHT_22_PENALTY=3.121175
PRIMER_LEFT_22_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_22_SEQUENCE=CTTCAGTGTTTTAGGGATGATGG
PRIMER_LEFT_22=880,22
PRIMER_RIGHT_22=1469,23
PRIMER_LEFT_22_TM=60.244
PRIMER_RIGHT_22_TM=59.879
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=43.478
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.6000
PRIMER_RIGHT_22_END_STABILITY=8.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=590
PRIMER_PAIR_23_PENALTY=5.378008
PRIMER_LEFT_23_PENALTY=3.750661
PRIMER_RIGHT_23_PENALTY=1.627348
PRIMER_LEFT_23_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_23_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_23=677,20
PRIMER_RIGHT_23=1461,21
PRIMER_LEFT_23_TM=56.249
PRIMER_RIGHT_23_TM=59.373
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=785
PRIMER_PAIR_24_PENALTY=5.403939
PRIMER_LEFT_24_PENALTY=4.227072
PRIMER_RIGHT_24_PENALTY=1.176867
PRIMER_LEFT_24_SEQUENCE=AACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_24_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_24=753,23
PRIMER_RIGHT_24=1463,21
PRIMER_LEFT_24_TM=58.773
PRIMER_RIGHT_24_TM=60.177
PRIMER_LEFT_24_GC_PERCENT=43.478
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=711
PRIMER_PAIR_25_PENALTY=5.423173
PRIMER_LEFT_25_PENALTY=2.243575
PRIMER_RIGHT_25_PENALTY=3.179598
PRIMER_LEFT_25_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_25_SEQUENCE=TAGGGATGATGGGTGAGATT
PRIMER_LEFT_25=880,22
PRIMER_RIGHT_25=1458,20
PRIMER_LEFT_25_TM=60.244
PRIMER_RIGHT_25_TM=56.820
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=6.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=579
PRIMER_PAIR_26_PENALTY=5.440591
PRIMER_LEFT_26_PENALTY=3.813243
PRIMER_RIGHT_26_PENALTY=1.627348
PRIMER_LEFT_26_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_26_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_26=886,20
PRIMER_RIGHT_26=1461,21
PRIMER_LEFT_26_TM=56.187
PRIMER_RIGHT_26_TM=59.373
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=576
PRIMER_PAIR_27_PENALTY=5.452601
PRIMER_LEFT_27_PENALTY=3.750661
PRIMER_RIGHT_27_PENALTY=1.701940
PRIMER_LEFT_27_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_27_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_27=677,20
PRIMER_RIGHT_27=1466,21
PRIMER_LEFT_27_TM=56.249
PRIMER_RIGHT_27_TM=59.298
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=790
PRIMER_PAIR_28_PENALTY=5.458163
PRIMER_LEFT_28_PENALTY=4.281296
PRIMER_RIGHT_28_PENALTY=1.176867
PRIMER_LEFT_28_SEQUENCE=GCATCCTACGATCCTTTATGTC
PRIMER_RIGHT_28_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_28=767,22
PRIMER_RIGHT_28=1463,21
PRIMER_LEFT_28_TM=57.719
PRIMER_RIGHT_28_TM=60.177
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.3000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=697
PRIMER_PAIR_29_PENALTY=5.480433
PRIMER_LEFT_29_PENALTY=3.750661
PRIMER_RIGHT_29_PENALTY=1.729772
PRIMER_LEFT_29_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_29_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_29=677,20
PRIMER_RIGHT_29=1463,20
PRIMER_LEFT_29_TM=56.249
PRIMER_RIGHT_29_TM=58.270
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=9.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=787
PRIMER_PAIR_30_PENALTY=5.486748
PRIMER_LEFT_30_PENALTY=3.756976
PRIMER_RIGHT_30_PENALTY=1.729772
PRIMER_LEFT_30_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_30_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_30=914,20
PRIMER_RIGHT_30=1463,20
PRIMER_LEFT_30_TM=56.243
PRIMER_RIGHT_30_TM=58.270
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.3000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=550
PRIMER_PAIR_31_PENALTY=5.512702
PRIMER_LEFT_31_PENALTY=4.335835
PRIMER_RIGHT_31_PENALTY=1.176867
PRIMER_LEFT_31_SEQUENCE=ACCCAGTTCAAAACGTAACA
PRIMER_RIGHT_31_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_31=676,20
PRIMER_RIGHT_31=1463,21
PRIMER_LEFT_31_TM=55.664
PRIMER_RIGHT_31_TM=60.177
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.0000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=788
PRIMER_PAIR_32_PENALTY=5.515183
PRIMER_LEFT_32_PENALTY=3.813243
PRIMER_RIGHT_32_PENALTY=1.701940
PRIMER_LEFT_32_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_32_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_32=886,20
PRIMER_RIGHT_32=1466,21
PRIMER_LEFT_32_TM=56.187
PRIMER_RIGHT_32_TM=59.298
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=581
PRIMER_PAIR_33_PENALTY=5.543015
PRIMER_LEFT_33_PENALTY=3.813243
PRIMER_RIGHT_33_PENALTY=1.729772
PRIMER_LEFT_33_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_33_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_33=886,20
PRIMER_RIGHT_33=1463,20
PRIMER_LEFT_33_TM=56.187
PRIMER_RIGHT_33_TM=58.270
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=578
PRIMER_PAIR_34_PENALTY=5.590453
PRIMER_LEFT_34_PENALTY=3.750661
PRIMER_RIGHT_34_PENALTY=1.839792
PRIMER_LEFT_34_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_34_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_34=677,20
PRIMER_RIGHT_34=1464,21
PRIMER_LEFT_34_TM=56.249
PRIMER_RIGHT_34_TM=59.160
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=788
PRIMER_PAIR_35_PENALTY=5.596768
PRIMER_LEFT_35_PENALTY=3.756976
PRIMER_RIGHT_35_PENALTY=1.839792
PRIMER_LEFT_35_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_35_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_35=914,20
PRIMER_RIGHT_35=1464,21
PRIMER_LEFT_35_TM=56.243
PRIMER_RIGHT_35_TM=59.160
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.3000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=7.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=551
PRIMER_PAIR_36_PENALTY=5.618147
PRIMER_LEFT_36_PENALTY=4.441280
PRIMER_RIGHT_36_PENALTY=1.176867
PRIMER_LEFT_36_SEQUENCE=CGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_36_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_36=755,21
PRIMER_RIGHT_36=1463,21
PRIMER_LEFT_36_TM=56.559
PRIMER_RIGHT_36_TM=60.177
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=709
PRIMER_PAIR_37_PENALTY=5.653035
PRIMER_LEFT_37_PENALTY=3.813243
PRIMER_RIGHT_37_PENALTY=1.839792
PRIMER_LEFT_37_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_37_SEQUENCE=GTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_37=886,20
PRIMER_RIGHT_37=1464,21
PRIMER_LEFT_37_TM=56.187
PRIMER_RIGHT_37_TM=59.160
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=9.4000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=579
PRIMER_PAIR_38_PENALTY=5.663303
PRIMER_LEFT_38_PENALTY=4.035956
PRIMER_RIGHT_38_PENALTY=1.627348
PRIMER_LEFT_38_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_38_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_38=752,24
PRIMER_RIGHT_38=1461,21
PRIMER_LEFT_38_TM=59.964
PRIMER_RIGHT_38_TM=59.373
PRIMER_LEFT_38_GC_PERCENT=41.667
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=710
PRIMER_PAIR_39_PENALTY=5.710410
PRIMER_LEFT_39_PENALTY=2.243575
PRIMER_RIGHT_39_PENALTY=3.466835
PRIMER_LEFT_39_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_39_SEQUENCE=TCAGTGTTTTAGGGATGATGG
PRIMER_LEFT_39=880,22
PRIMER_RIGHT_39=1467,21
PRIMER_LEFT_39_TM=60.244
PRIMER_RIGHT_39_TM=57.533
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.6000
PRIMER_RIGHT_39_END_STABILITY=8.1000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=588
PRIMER_PAIR_40_PENALTY=5.717023
PRIMER_LEFT_40_PENALTY=4.540156
PRIMER_RIGHT_40_PENALTY=1.176867
PRIMER_LEFT_40_SEQUENCE=ACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_40_SEQUENCE=TGTTTTAGGGATGATGGGTGA
PRIMER_LEFT_40=754,22
PRIMER_RIGHT_40=1463,21
PRIMER_LEFT_40_TM=57.460
PRIMER_RIGHT_40_TM=60.177
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.9000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=710
PRIMER_PAIR_41_PENALTY=5.737896
PRIMER_LEFT_41_PENALTY=4.035956
PRIMER_RIGHT_41_PENALTY=1.701940
PRIMER_LEFT_41_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_41_SEQUENCE=CAGTGTTTTAGGGATGATGGG
PRIMER_LEFT_41=752,24
PRIMER_RIGHT_41=1466,21
PRIMER_LEFT_41_TM=59.964
PRIMER_RIGHT_41_TM=59.298
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=715
PRIMER_PAIR_42_PENALTY=5.765728
PRIMER_LEFT_42_PENALTY=4.035956
PRIMER_RIGHT_42_PENALTY=1.729772
PRIMER_LEFT_42_SEQUENCE=AAACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_42_SEQUENCE=TGTTTTAGGGATGATGGGTG
PRIMER_LEFT_42=752,24
PRIMER_RIGHT_42=1463,20
PRIMER_LEFT_42_TM=59.964
PRIMER_RIGHT_42_TM=58.270
PRIMER_LEFT_42_GC_PERCENT=41.667
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.9000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=712
PRIMER_PAIR_43_PENALTY=5.799533
PRIMER_LEFT_43_PENALTY=2.243575
PRIMER_RIGHT_43_PENALTY=3.555959
PRIMER_LEFT_43_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_43_SEQUENCE=AGTGTTTTAGGGATGATGGG
PRIMER_LEFT_43=880,22
PRIMER_RIGHT_43=1465,20
PRIMER_LEFT_43_TM=60.244
PRIMER_RIGHT_43_TM=56.444
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.6000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=586
PRIMER_PAIR_44_PENALTY=5.817285
PRIMER_LEFT_44_PENALTY=3.750661
PRIMER_RIGHT_44_PENALTY=2.066624
PRIMER_LEFT_44_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_44_SEQUENCE=TTTAGGGATGATGGGTGAGA
PRIMER_LEFT_44=677,20
PRIMER_RIGHT_44=1460,20
PRIMER_LEFT_44_TM=56.249
PRIMER_RIGHT_44_TM=57.933
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=784
PRIMER_PAIR_45_PENALTY=5.854420
PRIMER_LEFT_45_PENALTY=4.227072
PRIMER_RIGHT_45_PENALTY=1.627348
PRIMER_LEFT_45_SEQUENCE=AACGTCCTACAAAAGCATCCTAC
PRIMER_RIGHT_45_SEQUENCE=TTTTAGGGATGATGGGTGAGA
PRIMER_LEFT_45=753,23
PRIMER_RIGHT_45=1461,21
PRIMER_LEFT_45_TM=58.773
PRIMER_RIGHT_45_TM=59.373
PRIMER_LEFT_45_GC_PERCENT=43.478
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=709
PRIMER_PAIR_46_PENALTY=5.854880
PRIMER_LEFT_46_PENALTY=2.243575
PRIMER_RIGHT_46_PENALTY=3.611306
PRIMER_LEFT_46_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_46_SEQUENCE=AGTGTTTTAGGGATGATGGGT
PRIMER_LEFT_46=880,22
PRIMER_RIGHT_46=1465,21
PRIMER_LEFT_46_TM=60.244
PRIMER_RIGHT_46_TM=57.389
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.6000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=586
PRIMER_PAIR_47_PENALTY=5.860171
PRIMER_LEFT_47_PENALTY=3.750661
PRIMER_RIGHT_47_PENALTY=2.109510
PRIMER_LEFT_47_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_47_SEQUENCE=AGTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_47=677,20
PRIMER_RIGHT_47=1465,22
PRIMER_LEFT_47_TM=56.249
PRIMER_RIGHT_47_TM=60.110
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=9.4000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=789
PRIMER_PAIR_48_PENALTY=5.860171
PRIMER_LEFT_48_PENALTY=3.750661
PRIMER_RIGHT_48_PENALTY=2.109510
PRIMER_LEFT_48_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_48_SEQUENCE=CAGTGTTTTAGGGATGATGGGT
PRIMER_LEFT_48=677,20
PRIMER_RIGHT_48=1466,22
PRIMER_LEFT_48_TM=56.249
PRIMER_RIGHT_48_TM=60.110
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=790
PRIMER_PAIR_49_PENALTY=5.866486
PRIMER_LEFT_49_PENALTY=3.756976
PRIMER_RIGHT_49_PENALTY=2.109510
PRIMER_LEFT_49_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_49_SEQUENCE=AGTGTTTTAGGGATGATGGGTG
PRIMER_LEFT_49=914,20
PRIMER_RIGHT_49=1465,22
PRIMER_LEFT_49_TM=56.243
PRIMER_RIGHT_49_TM=60.110
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.455
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.3000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=552
=
SEQUENCE_ID=MAGIC.338_5_14909153_14909417_kn-0_po-0_12376_110589_TRUE
SEQUENCE_TARGET=950,364
SEQUENCE_TEMPLATE=GATAATTCCTGTTCTCGGAACCATCCCGGCTATNTTTGGACAAATNATGGCCTCCTATGTTATAACACAACTTGCAGGTGTGCAAGTTCAGATGGAGCCNATAGTGAATCTAGATTTNGATCATTACCGATTGCTTCATCAACGCCTTATTGAGCATGAGGAGACAGTTTATGGCACATCTGCNGAAGTCGAGGTATTCAGATTNGTCTCACTGATAATATAGTTNCTANAACTTGTTANGAAAAAGACTAATAGNAAAAAGAGATGGTATCTGAGNTATATGAGACACTTGAAACTNCTTGTGAAGTTAANAGCAGNTTCTTGAGAANTCNTCTAAACTTTTGTCCANNTCCTGAAANTGATTAAAAGTTTGTATCTCCTTTTGTTTTCTGTAGGTGGATGTTGAGGAAGTGATGTACATAGTGAAAGAGTTGTGGCACGGACGAAGTGCTAGANATGAGACTGCAAAAGATGTTGGCAGAGGGATGTGGCGAGCCATGAATGAGTTGATGCTCGTNAGGTANGTTGATTCACTCCTTTTACTAAGCTCAATGGAAATCTGACAANAAANNATAATGNGAAGTTATGANTAATCAGATGGGATGCAAANAAGCCAGCAACAGTCTCAAACTTGGTTCTTTTGAAGTTNAAAGAGGTTTGGTTTTGCTCAATGATAATNNNNNNNNNNNNNTTACATGACCTTTATCNNTTTGNTCNGGTTTTGTNACANTTTGAGATATGATGTATGTTGTNATAGGCGGATGAACATGAGACTAAGACTCTTGAGGAAGTGAAGGAAAGTGAAANAGAGTTCTTTGAAAGAGTTTCATGCGTACTGAAGAAGGCAGAGCTTGATTTCTACGGCTANAGAACCCNTTGAACTGAACATCAATCACACAGGCTTGAAGAACCCATTCATGACTTACAAAATTACTCATCTTAATGTNTTATTTNCTCATTTGATTAGCAAAANTTTTGTTTTNATCAATTAGTAANAACANTNNNNNGAGTTTATTATAGAGAAATTTCACTACTTTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTATNAANTTNGAAAACTAAAACATACNAAACCNTAAGAATACTATTTCTTTTTAATCTATCTATGTGGGTTTGAAGAAAAACAAAATACTNATANTACTACTATCTCNNNNGNAATTTCTAATGTGNTAANAAAAAANTATCAAANTGGAAGTTGNAGAAATTATNAANTANTATATAAAAGNTNANANCCACTNNNNNCANTTGATTGTAGNTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATATNNTCACGTTTCAAAGCNAGTGATGCATGAACNTGNGGAANGNTCTTAATAAANAATGTNCNANATCNGAANNTGGANAAATTATNAAGTANTATATAAANTTTTAGANNNANTANATNTATTGCNNATTNGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANTGTAAACAAATCNCATATCGGATTCTTGTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTTANATTTAGCNAANAAAAAAGTATCTTGAACTNATTTTTTTTGGTTCACCAATCATGCAAGCCACGTAACAGATGTTNCAGTNCAACATTTGTTANNGANTATNACCACTAACNNAATCCCAAATATGACAATCATAAATAGANTTAACTCAAAAGAGACCCAACGTATAACTGCCATGAAGATATTTGTAAATAAACCAGGTTACAAAATTTAATAATTGCACGGCTTNGTGTTGGATAAAACAACGACACTCCNNTTTNAAAGATCNAAAGTATACAAATTAAAAACGTGAATGTGTCAAAAGTTCACGATCAAATGCACAAGTCCCTGTCGGGAATTNNTTTTTTTCCTCNTCACAGTTTTNTTTTCGAGAGATCATTATACTGTATTTTTTCNTCACTTACTCGATGTTNNGTATTACTCCTCTTTTATTACATTACTTATTGTATATTTGTAACATCACAGTTTCATTTTGATGACGTTGCATGCATTTATTTTAGTGATCCAAATAAGTCGATGACTTTATGTCATGTTGTCAACGTCACTTGCAAATGAATATCTTTTGTGTACACCAGTTTATGTCATCCGNCGGAAAATGATNTTCTCAAATATAGGCATGCGTATACAAAGTATNTATTTCTAAATANTAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.925501
PRIMER_LEFT_0_PENALTY=0.260155
PRIMER_RIGHT_0_PENALTY=0.665346
PRIMER_LEFT_0_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_0_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_0=829,20
PRIMER_RIGHT_0=1681,20
PRIMER_LEFT_0_TM=59.740
PRIMER_RIGHT_0_TM=60.665
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=853
PRIMER_PAIR_1_PENALTY=1.343019
PRIMER_LEFT_1_PENALTY=0.260155
PRIMER_RIGHT_1_PENALTY=1.082864
PRIMER_LEFT_1_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_1_SEQUENCE=CATGGCAGTTATACGTTGGG
PRIMER_LEFT_1=829,20
PRIMER_RIGHT_1=1791,20
PRIMER_LEFT_1_TM=59.740
PRIMER_RIGHT_1_TM=58.917
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=963
PRIMER_PAIR_2_PENALTY=1.355027
PRIMER_LEFT_2_PENALTY=0.689681
PRIMER_RIGHT_2_PENALTY=0.665346
PRIMER_LEFT_2_SEQUENCE=ACACAGGCTTGAAGAACCCA
PRIMER_RIGHT_2_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_2=894,20
PRIMER_RIGHT_2=1681,20
PRIMER_LEFT_2_TM=60.690
PRIMER_RIGHT_2_TM=60.665
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=788
PRIMER_PAIR_3_PENALTY=1.499607
PRIMER_LEFT_3_PENALTY=0.260155
PRIMER_RIGHT_3_PENALTY=1.239452
PRIMER_LEFT_3_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_3_SEQUENCE=CATGGCAGTTATACGTTGGGT
PRIMER_LEFT_3=829,20
PRIMER_RIGHT_3=1791,21
PRIMER_LEFT_3_TM=59.740
PRIMER_RIGHT_3_TM=59.761
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=963
PRIMER_PAIR_4_PENALTY=1.607151
PRIMER_LEFT_4_PENALTY=0.260155
PRIMER_RIGHT_4_PENALTY=1.346996
PRIMER_LEFT_4_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_4_SEQUENCE=GTTACGTGGCTTGCATGATT
PRIMER_LEFT_4=829,20
PRIMER_RIGHT_4=1682,20
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=58.653
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=854
PRIMER_PAIR_5_PENALTY=1.752366
PRIMER_LEFT_5_PENALTY=0.260155
PRIMER_RIGHT_5_PENALTY=1.492211
PRIMER_LEFT_5_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_5_SEQUENCE=TGGCAGTTATACGTTGGGTCT
PRIMER_LEFT_5=829,20
PRIMER_RIGHT_5=1789,21
PRIMER_LEFT_5_TM=59.740
PRIMER_RIGHT_5_TM=59.508
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=961
PRIMER_PAIR_6_PENALTY=1.757818
PRIMER_LEFT_6_PENALTY=0.260155
PRIMER_RIGHT_6_PENALTY=1.497663
PRIMER_LEFT_6_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_6_SEQUENCE=TGGCAGTTATACGTTGGGTC
PRIMER_LEFT_6=829,20
PRIMER_RIGHT_6=1789,20
PRIMER_LEFT_6_TM=59.740
PRIMER_RIGHT_6_TM=58.502
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.1000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=961
PRIMER_PAIR_7_PENALTY=1.810983
PRIMER_LEFT_7_PENALTY=1.145637
PRIMER_RIGHT_7_PENALTY=0.665346
PRIMER_LEFT_7_SEQUENCE=TCACACAGGCTTGAAGAACC
PRIMER_RIGHT_7_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_7=892,20
PRIMER_RIGHT_7=1681,20
PRIMER_LEFT_7_TM=58.854
PRIMER_RIGHT_7_TM=60.665
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=790
PRIMER_PAIR_8_PENALTY=2.017295
PRIMER_LEFT_8_PENALTY=0.260155
PRIMER_RIGHT_8_PENALTY=1.757139
PRIMER_LEFT_8_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_8_SEQUENCE=TCATGGCAGTTATACGTTGGG
PRIMER_LEFT_8=829,20
PRIMER_RIGHT_8=1792,21
PRIMER_LEFT_8_TM=59.740
PRIMER_RIGHT_8_TM=60.757
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=964
PRIMER_PAIR_9_PENALTY=2.019498
PRIMER_LEFT_9_PENALTY=1.354152
PRIMER_RIGHT_9_PENALTY=0.665346
PRIMER_LEFT_9_SEQUENCE=ACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_9_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_9=896,20
PRIMER_RIGHT_9=1681,20
PRIMER_LEFT_9_TM=58.646
PRIMER_RIGHT_9_TM=60.665
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=786
PRIMER_PAIR_10_PENALTY=2.036677
PRIMER_LEFT_10_PENALTY=0.689681
PRIMER_RIGHT_10_PENALTY=1.346996
PRIMER_LEFT_10_SEQUENCE=ACACAGGCTTGAAGAACCCA
PRIMER_RIGHT_10_SEQUENCE=GTTACGTGGCTTGCATGATT
PRIMER_LEFT_10=894,20
PRIMER_RIGHT_10=1682,20
PRIMER_LEFT_10_TM=60.690
PRIMER_RIGHT_10_TM=58.653
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=789
PRIMER_PAIR_11_PENALTY=2.228500
PRIMER_LEFT_11_PENALTY=1.145637
PRIMER_RIGHT_11_PENALTY=1.082864
PRIMER_LEFT_11_SEQUENCE=TCACACAGGCTTGAAGAACC
PRIMER_RIGHT_11_SEQUENCE=CATGGCAGTTATACGTTGGG
PRIMER_LEFT_11=892,20
PRIMER_RIGHT_11=1791,20
PRIMER_LEFT_11_TM=58.854
PRIMER_RIGHT_11_TM=58.917
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=10.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=900
PRIMER_PAIR_12_PENALTY=2.380532
PRIMER_LEFT_12_PENALTY=1.715186
PRIMER_RIGHT_12_PENALTY=0.665346
PRIMER_LEFT_12_SEQUENCE=TGCGTACTGAAGAAGGCAGAG
PRIMER_RIGHT_12_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_12=829,21
PRIMER_RIGHT_12=1681,20
PRIMER_LEFT_12_TM=60.715
PRIMER_RIGHT_12_TM=60.665
PRIMER_LEFT_12_GC_PERCENT=52.381
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=853
PRIMER_PAIR_13_PENALTY=2.385089
PRIMER_LEFT_13_PENALTY=1.145637
PRIMER_RIGHT_13_PENALTY=1.239452
PRIMER_LEFT_13_SEQUENCE=TCACACAGGCTTGAAGAACC
PRIMER_RIGHT_13_SEQUENCE=CATGGCAGTTATACGTTGGGT
PRIMER_LEFT_13=892,20
PRIMER_RIGHT_13=1791,21
PRIMER_LEFT_13_TM=58.854
PRIMER_RIGHT_13_TM=59.761
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=900
PRIMER_PAIR_14_PENALTY=2.410537
PRIMER_LEFT_14_PENALTY=0.260155
PRIMER_RIGHT_14_PENALTY=2.150382
PRIMER_LEFT_14_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_14_SEQUENCE=ATGGCAGTTATACGTTGGGTC
PRIMER_LEFT_14=829,20
PRIMER_RIGHT_14=1790,21
PRIMER_LEFT_14_TM=59.740
PRIMER_RIGHT_14_TM=58.850
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=962
PRIMER_PAIR_15_PENALTY=2.437016
PRIMER_LEFT_15_PENALTY=1.354152
PRIMER_RIGHT_15_PENALTY=1.082864
PRIMER_LEFT_15_SEQUENCE=ACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_15_SEQUENCE=CATGGCAGTTATACGTTGGG
PRIMER_LEFT_15=896,20
PRIMER_RIGHT_15=1791,20
PRIMER_LEFT_15_TM=58.646
PRIMER_RIGHT_15_TM=58.917
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=10.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=896
PRIMER_PAIR_16_PENALTY=2.463966
PRIMER_LEFT_16_PENALTY=1.798620
PRIMER_RIGHT_16_PENALTY=0.665346
PRIMER_LEFT_16_SEQUENCE=ATCACACAGGCTTGAAGAACC
PRIMER_RIGHT_16_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_16=891,21
PRIMER_RIGHT_16=1681,20
PRIMER_LEFT_16_TM=59.201
PRIMER_RIGHT_16_TM=60.665
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=791
PRIMER_PAIR_17_PENALTY=2.471041
PRIMER_LEFT_17_PENALTY=0.260155
PRIMER_RIGHT_17_PENALTY=2.210886
PRIMER_LEFT_17_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_17_SEQUENCE=ATGGCAGTTATACGTTGGGTCT
PRIMER_LEFT_17=829,20
PRIMER_RIGHT_17=1790,22
PRIMER_LEFT_17_TM=59.740
PRIMER_RIGHT_17_TM=59.789
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.455
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=962
PRIMER_PAIR_18_PENALTY=2.492633
PRIMER_LEFT_18_PENALTY=1.145637
PRIMER_RIGHT_18_PENALTY=1.346996
PRIMER_LEFT_18_SEQUENCE=TCACACAGGCTTGAAGAACC
PRIMER_RIGHT_18_SEQUENCE=GTTACGTGGCTTGCATGATT
PRIMER_LEFT_18=892,20
PRIMER_RIGHT_18=1682,20
PRIMER_LEFT_18_TM=58.854
PRIMER_RIGHT_18_TM=58.653
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=791
PRIMER_PAIR_19_PENALTY=2.548654
PRIMER_LEFT_19_PENALTY=1.883308
PRIMER_RIGHT_19_PENALTY=0.665346
PRIMER_LEFT_19_SEQUENCE=ATAGGCGGATGAACATGAGA
PRIMER_RIGHT_19_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_19=753,20
PRIMER_RIGHT_19=1681,20
PRIMER_LEFT_19_TM=58.117
PRIMER_RIGHT_19_TM=60.665
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=929
PRIMER_PAIR_20_PENALTY=2.593604
PRIMER_LEFT_20_PENALTY=1.354152
PRIMER_RIGHT_20_PENALTY=1.239452
PRIMER_LEFT_20_SEQUENCE=ACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_20_SEQUENCE=CATGGCAGTTATACGTTGGGT
PRIMER_LEFT_20=896,20
PRIMER_RIGHT_20=1791,21
PRIMER_LEFT_20_TM=58.646
PRIMER_RIGHT_20_TM=59.761
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.4000
PRIMER_RIGHT_20_END_STABILITY=9.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=896
PRIMER_PAIR_21_PENALTY=2.643300
PRIMER_LEFT_21_PENALTY=1.145637
PRIMER_RIGHT_21_PENALTY=1.497663
PRIMER_LEFT_21_SEQUENCE=TCACACAGGCTTGAAGAACC
PRIMER_RIGHT_21_SEQUENCE=TGGCAGTTATACGTTGGGTC
PRIMER_LEFT_21=892,20
PRIMER_RIGHT_21=1789,20
PRIMER_LEFT_21_TM=58.854
PRIMER_RIGHT_21_TM=58.502
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=9.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=898
PRIMER_PAIR_22_PENALTY=2.668014
PRIMER_LEFT_22_PENALTY=0.260155
PRIMER_RIGHT_22_PENALTY=2.407858
PRIMER_LEFT_22_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_22_SEQUENCE=GGCAGTTATACGTTGGGTCTC
PRIMER_LEFT_22=829,20
PRIMER_RIGHT_22=1788,21
PRIMER_LEFT_22_TM=59.740
PRIMER_RIGHT_22_TM=58.592
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.1000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=960
PRIMER_PAIR_23_PENALTY=2.690373
PRIMER_LEFT_23_PENALTY=0.260155
PRIMER_RIGHT_23_PENALTY=2.430217
PRIMER_LEFT_23_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_23_SEQUENCE=TTCATGGCAGTTATACGTTGG
PRIMER_LEFT_23=829,20
PRIMER_RIGHT_23=1793,21
PRIMER_LEFT_23_TM=59.740
PRIMER_RIGHT_23_TM=58.570
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=965
PRIMER_PAIR_24_PENALTY=2.701148
PRIMER_LEFT_24_PENALTY=1.354152
PRIMER_RIGHT_24_PENALTY=1.346996
PRIMER_LEFT_24_SEQUENCE=ACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_24_SEQUENCE=GTTACGTGGCTTGCATGATT
PRIMER_LEFT_24=896,20
PRIMER_RIGHT_24=1682,20
PRIMER_LEFT_24_TM=58.646
PRIMER_RIGHT_24_TM=58.653
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=787
PRIMER_PAIR_25_PENALTY=2.712173
PRIMER_LEFT_25_PENALTY=0.260155
PRIMER_RIGHT_25_PENALTY=2.452017
PRIMER_LEFT_25_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_25_SEQUENCE=GGCAGTTATACGTTGGGTCTCT
PRIMER_LEFT_25=829,20
PRIMER_RIGHT_25=1788,22
PRIMER_LEFT_25_TM=59.740
PRIMER_RIGHT_25_TM=59.548
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=960
PRIMER_PAIR_26_PENALTY=2.720159
PRIMER_LEFT_26_PENALTY=0.260155
PRIMER_RIGHT_26_PENALTY=2.460004
PRIMER_LEFT_26_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_26_SEQUENCE=GTTACGTGGCTTGCATGATTG
PRIMER_LEFT_26=829,20
PRIMER_RIGHT_26=1682,21
PRIMER_LEFT_26_TM=59.740
PRIMER_RIGHT_26_TM=61.460
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=854
PRIMER_PAIR_27_PENALTY=2.720159
PRIMER_LEFT_27_PENALTY=0.260155
PRIMER_RIGHT_27_PENALTY=2.460004
PRIMER_LEFT_27_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_27_SEQUENCE=TGTTACGTGGCTTGCATGATT
PRIMER_LEFT_27=829,20
PRIMER_RIGHT_27=1683,21
PRIMER_LEFT_27_TM=59.740
PRIMER_RIGHT_27_TM=61.460
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=855
PRIMER_PAIR_28_PENALTY=2.724322
PRIMER_LEFT_28_PENALTY=0.260155
PRIMER_RIGHT_28_PENALTY=2.464167
PRIMER_LEFT_28_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_28_SEQUENCE=CTTCATGGCAGTTATACGTTGG
PRIMER_LEFT_28=829,20
PRIMER_RIGHT_28=1794,22
PRIMER_LEFT_28_TM=59.740
PRIMER_RIGHT_28_TM=59.536
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=966
PRIMER_PAIR_29_PENALTY=2.798050
PRIMER_LEFT_29_PENALTY=1.715186
PRIMER_RIGHT_29_PENALTY=1.082864
PRIMER_LEFT_29_SEQUENCE=TGCGTACTGAAGAAGGCAGAG
PRIMER_RIGHT_29_SEQUENCE=CATGGCAGTTATACGTTGGG
PRIMER_LEFT_29=829,21
PRIMER_RIGHT_29=1791,20
PRIMER_LEFT_29_TM=60.715
PRIMER_RIGHT_29_TM=58.917
PRIMER_LEFT_29_GC_PERCENT=52.381
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=10.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=963
PRIMER_PAIR_30_PENALTY=2.810864
PRIMER_LEFT_30_PENALTY=2.145518
PRIMER_RIGHT_30_PENALTY=0.665346
PRIMER_LEFT_30_SEQUENCE=GCGTACTGAAGAAGGCAGAG
PRIMER_RIGHT_30_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_30=830,20
PRIMER_RIGHT_30=1681,20
PRIMER_LEFT_30_TM=57.854
PRIMER_RIGHT_30_TM=60.665
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=852
PRIMER_PAIR_31_PENALTY=2.846363
PRIMER_LEFT_31_PENALTY=1.354152
PRIMER_RIGHT_31_PENALTY=1.492211
PRIMER_LEFT_31_SEQUENCE=ACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_31_SEQUENCE=TGGCAGTTATACGTTGGGTCT
PRIMER_LEFT_31=896,20
PRIMER_RIGHT_31=1789,21
PRIMER_LEFT_31_TM=58.646
PRIMER_RIGHT_31_TM=59.508
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=894
PRIMER_PAIR_32_PENALTY=2.851815
PRIMER_LEFT_32_PENALTY=1.354152
PRIMER_RIGHT_32_PENALTY=1.497663
PRIMER_LEFT_32_SEQUENCE=ACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_32_SEQUENCE=TGGCAGTTATACGTTGGGTC
PRIMER_LEFT_32=896,20
PRIMER_RIGHT_32=1789,20
PRIMER_LEFT_32_TM=58.646
PRIMER_RIGHT_32_TM=58.502
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=9.1000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=894
PRIMER_PAIR_33_PENALTY=2.881484
PRIMER_LEFT_33_PENALTY=1.798620
PRIMER_RIGHT_33_PENALTY=1.082864
PRIMER_LEFT_33_SEQUENCE=ATCACACAGGCTTGAAGAACC
PRIMER_RIGHT_33_SEQUENCE=CATGGCAGTTATACGTTGGG
PRIMER_LEFT_33=891,21
PRIMER_RIGHT_33=1791,20
PRIMER_LEFT_33_TM=59.201
PRIMER_RIGHT_33_TM=58.917
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=901
PRIMER_PAIR_34_PENALTY=2.902776
PRIMER_LEFT_34_PENALTY=1.145637
PRIMER_RIGHT_34_PENALTY=1.757139
PRIMER_LEFT_34_SEQUENCE=TCACACAGGCTTGAAGAACC
PRIMER_RIGHT_34_SEQUENCE=TCATGGCAGTTATACGTTGGG
PRIMER_LEFT_34=892,20
PRIMER_RIGHT_34=1792,21
PRIMER_LEFT_34_TM=58.854
PRIMER_RIGHT_34_TM=60.757
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=10.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=901
PRIMER_PAIR_35_PENALTY=2.909219
PRIMER_LEFT_35_PENALTY=2.243873
PRIMER_RIGHT_35_PENALTY=0.665346
PRIMER_LEFT_35_SEQUENCE=CAGAGCTTGATTTCTACGGCT
PRIMER_RIGHT_35_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_35=845,21
PRIMER_RIGHT_35=1681,20
PRIMER_LEFT_35_TM=58.756
PRIMER_RIGHT_35_TM=60.665
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=11.4000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=837
PRIMER_PAIR_36_PENALTY=2.915583
PRIMER_LEFT_36_PENALTY=2.250237
PRIMER_RIGHT_36_PENALTY=0.665346
PRIMER_LEFT_36_SEQUENCE=GGCAGAGCTTGATTTCTACGG
PRIMER_RIGHT_36_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_36=843,21
PRIMER_RIGHT_36=1681,20
PRIMER_LEFT_36_TM=61.250
PRIMER_RIGHT_36_TM=60.665
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.9000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=839
PRIMER_PAIR_37_PENALTY=2.938628
PRIMER_LEFT_37_PENALTY=2.273283
PRIMER_RIGHT_37_PENALTY=0.665346
PRIMER_LEFT_37_SEQUENCE=GCAGAGCTTGATTTCTACGG
PRIMER_RIGHT_37_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_37=844,20
PRIMER_RIGHT_37=1681,20
PRIMER_LEFT_37_TM=57.727
PRIMER_RIGHT_37_TM=60.665
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=838
PRIMER_PAIR_38_PENALTY=2.954638
PRIMER_LEFT_38_PENALTY=1.715186
PRIMER_RIGHT_38_PENALTY=1.239452
PRIMER_LEFT_38_SEQUENCE=TGCGTACTGAAGAAGGCAGAG
PRIMER_RIGHT_38_SEQUENCE=CATGGCAGTTATACGTTGGGT
PRIMER_LEFT_38=829,21
PRIMER_RIGHT_38=1791,21
PRIMER_LEFT_38_TM=60.715
PRIMER_RIGHT_38_TM=59.761
PRIMER_LEFT_38_GC_PERCENT=52.381
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=963
PRIMER_PAIR_39_PENALTY=3.038072
PRIMER_LEFT_39_PENALTY=1.798620
PRIMER_RIGHT_39_PENALTY=1.239452
PRIMER_LEFT_39_SEQUENCE=ATCACACAGGCTTGAAGAACC
PRIMER_RIGHT_39_SEQUENCE=CATGGCAGTTATACGTTGGGT
PRIMER_LEFT_39=891,21
PRIMER_RIGHT_39=1791,21
PRIMER_LEFT_39_TM=59.201
PRIMER_RIGHT_39_TM=59.761
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=9.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=901
PRIMER_PAIR_40_PENALTY=3.062182
PRIMER_LEFT_40_PENALTY=1.715186
PRIMER_RIGHT_40_PENALTY=1.346996
PRIMER_LEFT_40_SEQUENCE=TGCGTACTGAAGAAGGCAGAG
PRIMER_RIGHT_40_SEQUENCE=GTTACGTGGCTTGCATGATT
PRIMER_LEFT_40=829,21
PRIMER_RIGHT_40=1682,20
PRIMER_LEFT_40_TM=60.715
PRIMER_RIGHT_40_TM=58.653
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=6.7000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=854
PRIMER_PAIR_41_PENALTY=3.075883
PRIMER_LEFT_41_PENALTY=2.410537
PRIMER_RIGHT_41_PENALTY=0.665346
PRIMER_LEFT_41_SEQUENCE=CACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_41_SEQUENCE=TTACGTGGCTTGCATGATTG
PRIMER_LEFT_41=895,21
PRIMER_RIGHT_41=1681,20
PRIMER_LEFT_41_TM=61.411
PRIMER_RIGHT_41_TM=60.665
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.4000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=787
PRIMER_PAIR_42_PENALTY=3.111291
PRIMER_LEFT_42_PENALTY=1.354152
PRIMER_RIGHT_42_PENALTY=1.757139
PRIMER_LEFT_42_SEQUENCE=ACAGGCTTGAAGAACCCATT
PRIMER_RIGHT_42_SEQUENCE=TCATGGCAGTTATACGTTGGG
PRIMER_LEFT_42=896,20
PRIMER_RIGHT_42=1792,21
PRIMER_LEFT_42_TM=58.646
PRIMER_RIGHT_42_TM=60.757
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=10.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=897
PRIMER_PAIR_43_PENALTY=3.119899
PRIMER_LEFT_43_PENALTY=0.689681
PRIMER_RIGHT_43_PENALTY=2.430217
PRIMER_LEFT_43_SEQUENCE=ACACAGGCTTGAAGAACCCA
PRIMER_RIGHT_43_SEQUENCE=TTCATGGCAGTTATACGTTGG
PRIMER_LEFT_43=894,20
PRIMER_RIGHT_43=1793,21
PRIMER_LEFT_43_TM=60.690
PRIMER_RIGHT_43_TM=58.570
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=900
PRIMER_PAIR_44_PENALTY=3.145616
PRIMER_LEFT_44_PENALTY=1.798620
PRIMER_RIGHT_44_PENALTY=1.346996
PRIMER_LEFT_44_SEQUENCE=ATCACACAGGCTTGAAGAACC
PRIMER_RIGHT_44_SEQUENCE=GTTACGTGGCTTGCATGATT
PRIMER_LEFT_44=891,21
PRIMER_RIGHT_44=1682,20
PRIMER_LEFT_44_TM=59.201
PRIMER_RIGHT_44_TM=58.653
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=792
PRIMER_PAIR_45_PENALTY=3.149685
PRIMER_LEFT_45_PENALTY=0.689681
PRIMER_RIGHT_45_PENALTY=2.460004
PRIMER_LEFT_45_SEQUENCE=ACACAGGCTTGAAGAACCCA
PRIMER_RIGHT_45_SEQUENCE=GTTACGTGGCTTGCATGATTG
PRIMER_LEFT_45=894,20
PRIMER_RIGHT_45=1682,21
PRIMER_LEFT_45_TM=60.690
PRIMER_RIGHT_45_TM=61.460
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.4000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=789
PRIMER_PAIR_46_PENALTY=3.149685
PRIMER_LEFT_46_PENALTY=0.689681
PRIMER_RIGHT_46_PENALTY=2.460004
PRIMER_LEFT_46_SEQUENCE=ACACAGGCTTGAAGAACCCA
PRIMER_RIGHT_46_SEQUENCE=TGTTACGTGGCTTGCATGATT
PRIMER_LEFT_46=894,20
PRIMER_RIGHT_46=1683,21
PRIMER_LEFT_46_TM=60.690
PRIMER_RIGHT_46_TM=61.460
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.4000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=790
PRIMER_PAIR_47_PENALTY=3.153848
PRIMER_LEFT_47_PENALTY=0.689681
PRIMER_RIGHT_47_PENALTY=2.464167
PRIMER_LEFT_47_SEQUENCE=ACACAGGCTTGAAGAACCCA
PRIMER_RIGHT_47_SEQUENCE=CTTCATGGCAGTTATACGTTGG
PRIMER_LEFT_47=894,20
PRIMER_RIGHT_47=1794,22
PRIMER_LEFT_47_TM=60.690
PRIMER_RIGHT_47_TM=59.536
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.455
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.4000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=901
PRIMER_PAIR_48_PENALTY=3.160299
PRIMER_LEFT_48_PENALTY=0.260155
PRIMER_RIGHT_48_PENALTY=2.900144
PRIMER_LEFT_48_SEQUENCE=TGCGTACTGAAGAAGGCAGA
PRIMER_RIGHT_48_SEQUENCE=TCATGGCAGTTATACGTTGG
PRIMER_LEFT_48=829,20
PRIMER_RIGHT_48=1792,20
PRIMER_LEFT_48_TM=59.740
PRIMER_RIGHT_48_TM=57.100
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=964
PRIMER_PAIR_49_PENALTY=3.171329
PRIMER_LEFT_49_PENALTY=2.088466
PRIMER_RIGHT_49_PENALTY=1.082864
PRIMER_LEFT_49_SEQUENCE=CAATCACACAGGCTTGAAGAA
PRIMER_RIGHT_49_SEQUENCE=CATGGCAGTTATACGTTGGG
PRIMER_LEFT_49=889,21
PRIMER_RIGHT_49=1791,20
PRIMER_LEFT_49_TM=58.912
PRIMER_RIGHT_49_TM=58.917
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=10.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=903
=
SEQUENCE_ID=MAGIC.338_4_4380257_4380363_ler-0_tsu-0_13467_65754_TRUE
SEQUENCE_TARGET=950,206
SEQUENCE_TEMPLATE=NAAAGCCTCTNAGGTAACNCAAAGNAAAAANGTTNAAAAGCTCCCTNNGNCAAACAACCNNTAAGTGTTAANACCCCTTGGNAAAATANAAAATTCCCTCTCGNGAAANTCGTGTTNATAAAANAAATTGGGACAATCCCCNANTNTTATGGTCATAGCCATNAAAGAGTTAANTTGNGCCGNAAGCCTCATCAACATTCGGTGTGATTTCGTCTTGGTNNNNNNTAGCAATGGTCGTTCCTTGCTCGCGAGTTTNNGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANACATTTTGGCGANTTCGAGATCAGCTTGCAGGAACTTANNNNNNNNNNNNNNNNGNCACNTTNACNAGNAAATTAGNCCTCCGACCAAGTNAANTTACTCGAANNACNGGNGGCTTATGTTGATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNCNATGTTGCACAAAGGAGGTGGTCAAATCTTGACATNAATTTGTNGTTNAANNNNNNNNNNNNNNNNNNNNGTCAAAAGAATAAACTTGTNNNNNNNNNNNNTGAATTGATTGTGGAAGTTATTTTGTGAATTAATNCTATAATTAATAGCCAAATAATGGCACATTGGAAGAAGGAAAGAGACTATAAAAGAGAGGTGAAAACTCCANNAAAANGGGCTAGAGATTCTACATTTTCACCACAAATCTTAATATTAAACATTGTTATTTTCTGATAAATACACAAAAGATTATTTCTTCCCTTTTTATGTAAGATCTTTATACAAGAAATATTATACCATTTTTTATGTACAATTTCTTCGTACATTTCTATATACTTACTTATACTTTTATTATGCACATTTGTCTTGTACTTTTGTACTCGAGANNTTTNGCCCACAAATAATNNNNATACTTGAGTGTNTGAATCCNACATNCANNNNNCNNNNCTCCGGTNGATCAATATGTNGCTCAAACTNGATTTGNTGCTCATAGTCAGCTTGAGTAAAGTACTCATGTTGCTCATNTTCAGCTTNANTATATTCCGNTTNATNNTCTCGAGNTCCTCCAATGTCGATGTTCAAGGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14037649_14037795_kn-0_ler-0_23179_1195_TRUE
SEQUENCE_TARGET=950,246
SEQUENCE_TEMPLATE=TNNCCTCAAAGGNGTGAAGCTTTTTCNTGTTTNTCGAGAAAGGANATGNCAANNGTCGCATNAAANACNAAAANCCCATTAAATNNNNNNNNNANAGTTNAGGCNCAAGCCNANNNAANAAGCNGAANTGATTAAACCNGAGTNGGGGANTTCGNNNNNNNAANCGAGCTCGGTTCTCGCNTTTTAAGAGAAAGGTNNNNNNNNNNNNAAAATCTNGAAACTTAGNNACANGGCNANAAGNTNTNTAAAGACGGACTNTNGNAANATNATAGNNNNAAAAGGTTTAAAAGCCTCCTCNGGCAANTACTTNTTNAAAAATNNAGCNTCNNNNNNNNNCANCNNATTTATCCAAATTTAATTTCTAGTTTTGGAAGTGTTTGGGANAGCCCCNAATTAAAGTAGATACAACCACTAGGAATCCGTAATCTTCTCGGGGTCAACGATAGCCNGATCGGCTTCGGTTGGNTCAACAACAGCTGACTCAGTTTTGGTTGGTTCACNGGCAGAGAGTAGCTTAGAAGAGTTTCCCGGCTCAGTTCCGGCTTCATGTTCAACATCCGGGATGATCACTGTCTCACTNTTCACGANCGGACAGTAGTATCGACCANTTCACTAGAGCCTGAAGNGAACGTTTCAAGTCAGCAANAAACACAAATACAACAACTNNNGNNNATAANGNTNCAGATNTAAAAGNTTANCANCCCAATCTAAGCCTGATTCACTCAAANNCANNGNNNNGNNNTAANANTCCTCGAAGTGTTGAGGGTTAAAGGACAAGGTGGGAAGAGNAGCAAGGTGGCCTCTTTTCTCAGCTAAAACAGCCTCCAGTTTGGACCTTTCTTGTTCCTCAGAGAAATCTTCCTCAAGGGTTTGGTCCAACANAAGCAGGNTNCTTTCGTGCTNNTNNNNNNNNNNNTNNAGCTCCGATNTGGCNNTCTCNGTTTGAATGANNAGGATAGCNCCTTGNATCANNTCCATNCCGCGACNTTTCACNTCTCTTNNNNNNNNNNNNNNNNNNNTGGCTAATTCAGCATCNNANNNNNNNNNNNNNNNNTTGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNAGTCGAGAGTTCNTCCTNNAGCTTGTCNTANNTTTGNTACTGATCNTGCAACTCGCCATTNGANNTCATGAGCTTNNNNNNNAGATGNTCGNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAGAGGAGGAGCNNNNNNNNNNNNNNNGNGAAGANAANTTGCCTTTCCAAGCATCNATNTCCNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNGATCATGTNNTTAAGCTCNNTGTTCGCCTNAGCAANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACCCGANATGAATGATGNGCNTGGAGTAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGATNGTGTNTTTCCNNGTTTTGAAGCNNNGNAANACGTTGCCCTCTTTCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCGAGNGAAATNAANCCNTNGTTNGAACCTNTAAGCNATTTNGTNTGAGGAACGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTNGATCAGNCTTCTCNCCACTAGGTNGNNGAGAAGGNNNNNNNNNNNNNNNNGAGGAGGAGNACCCATCGATCNAGATGGAGGAGATGATCGNNGAGAGCGATCAGTNCGAGCCNTTGACTTTTCCCTCCAAGGAGAAGAGCTGTTNCGAGCTGGTCTCTCNCTCNNTNTNNNNATNAAGTCAGCGATTGGCTTGCTGCTCAGTCCATCACGGGCTNAAGTCCCGTGTGGTNTGGGAGGANGNGGTGGAAGATGATGGAGTTGTCCAGGAGGACTCATNTNGAGNNNNNNNNNNNNNNNNNNNNNNNNGTCGAGGGTAAACAAATTCCAAAGGGTTTCACCTTCGATGACCACTTTGAAGAANNNNNNNNATTCTTCAGTCGGAAGNGNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4643679_4643690_bur-0_rsch-4_8824_68949_TRUE
SEQUENCE_TARGET=950,111
SEQUENCE_TEMPLATE=ATATCANGTTANTTTNANNTTAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGTCTANTACCCCCCNNNNTTNAAAACCACTCGCTCNAAAANTCCTCTCAAACACTATAATCGAGACAANTNCNTTNACCATGTTTTCTGANGCCGNTAGAGTTTTTGCTCGNCGGCGTCGNGATATTGTTTGCTTTTCTCAATCCTTTCGCAACCTTTCTNGATTCTTACTTCATTTGTTTATAATCTTNTACCATNNNNNNNNNCTTTCACCTTGCTTCCATGGTGTTCCTTTGTACCGCGATAACATTAATAGAGGTTTTCNGGCGAACCCTNTATCCCTCATNAGGCCCGTGAACTCNGTGAAGGAGGAACCNATGTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_14598904_14599062_ct-1_sf-2_63940_932001_TRUE
SEQUENCE_TARGET=950,258
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACTATTCGAAAGACGAGGAGNTATTGGTCTACAAGTACANGCATGGTGAGTCATAAACTATCTTGTNGNTAATGNAANCCTTTTGAGTCACGAAAAAGATTCTTCNTTTTTGTATTTTCCGAACAGGGGACAGAGAAGTTGACTAGGAAANGCGGATGAAGATCGTNANTAGGACACATCTTTAGTCACTCTCTTCNACCTCCTAACTCATANNNNNNNNNNNNNNNNNNNNTATGCTCCTGCANTGCTTGAGACAGTTGGGCACAAGGCAAGTANTTGTCAAANTGAAGGTATTTGTGACCCGACTTGCCTTNTACAAGTAANTAGATTNTTGGCTTNTTACTTGCCTTGTCAAAACGAGTACTTGTTTTGACAAGTACTTGTCTAAANTATGATTACNTACAAGTTATTTGACCCGCCNTGTTACTTNNNAAAATTAAATANTTCTACTTGAACCTAAAATATTTAGAANAAAAAAANCTATTTNATACANAATATTTAAGATTAATGAGTTTTGTTANAAGAAATTTTGGATTTTTANTTTTTTATGNNCCAAATCAAAATTNTAAATCTATTATAATAATTTAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_1626847_1627221_edi-0_wu-0_895_240492_TRUE
SEQUENCE_TARGET=950,474
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_11820824_11821349_tsu-0_zu-0_3881_163193_TRUE
SEQUENCE_TARGET=950,625
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAAAANCTTGANAAANGGCTACGNAAAATTNTTTCCGNTTCCGCTAAGATAATTATAATATTGGGTAAAATCTTCTNGGTTATTTGGTGANCTTTGGTNTTCACCTTGANCNGGTTCTNCTTGAANTTGTTTTCCATNAGATTGANTTTNTTGATAATGAGATGCTNGATATTNGGATTCTTCACCATCTTCTTCATGTTGNTTTGTTTGTGCTCTTTGTTCCAAAATTCTTTTNNTCTCATTTTCAATATATGCACGAGAGGATGGATCACTTATNAATTCAAATCTNCAAATANTATTTTANTNTCTTCTTTCNTTCTAGCTANNTCGATCTTCTGNCTTTGAATTTNNTTTCTTTNAGAANTACCTTTAGTAATAGCTTTGATAAGTTTATCATTTTGNTCCAAANGTTGTTNAANAGNTCTTNAGATTGTTGTTTTNNNTNTGCNTTTTNNCAAACCCATAGGCCTTNAAGATAAACTAAAATTGGCATNTTCACTATCCNTATTTAGATCAATTGAATTCATANCAGNGNAAGNTGATNACTCGGTATTGATTGAAAATGATNAAGATGACGTAACATNNCGACCNTCTCCTTGGAATGCAGCNGNTGTCTTCATGTTATCATTAGNATTTCTCNATNCCTTTAANAATAGGCCATACATGATCAAATTTGAATCCTCTNTTGTATTTTTCATATTGAGTTAATAACANNTTGACAATNAACAAATAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAANTTANTAAACGCAATAATTTATAATTGAGTACTTACAANGTNTTCTTCGGANGCACTGCTAGGATCTTTATACTNAATTTGGTTAACACATCCTCTCAGCTTTGAGACTNTAGAAAGAATTGACGTCATTCGAGTTTGTAAAGATCTTTTTGNTCTTCGTTGAAGANGAAAANNATCNGATTTATGATACTCGGCTTNAACTCTTGTCCATAGTTGATCTCNTGATTGNTTTATTCNTATTATTNNATTTTGGGAAACATCAAGATACACATGACACAATTGTATATCTTCTTGAATAGAATATGACAAAGAGCNACTTGAACTCATTTTTNAAAAATATAANAANTAANAAAATGAGTTNGAGATGTGTGATTATTCANAGAGTANTATATATTTATANNGAGAACATAAAGNAATACTANNAGTTAGATCTTGGTAAATTCAACGGTTAAGATTAANTTANNTNTTTTTCTAAAGAAGTGATACTTGATACANAACATGAAATGACTAATCAATGNANCGNTAAGATCTTGATNAANAAATTCAACNNCTAAGATGAACTCNTCTTTTTCTCATGAGGTATTNATTGATTTACGATATGAAATGACTNATCAATGCAANGGTTAGATCCTCTTCAAAANANTTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4699985_4700051_rsch-4_kn-0_4779_20698_TRUE
SEQUENCE_TARGET=950,166
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTANTACGNTGGATTTCAGCNTCGATGNNTCTTTGAGANTCTAAGTCAATTGATCCAAACTCAGNAAAANCTTNNGAATCCTATAATAATTAATAAAGTANAAAACAATGAATAACTNTCGANAATTTTCCAATCAAANTACAAAATTGATTCATAANCAATAAANCANCTTACCATTACGAATNTATATCTCANAGACTTTGNGATTTTGGTTTGAAATTCTTCAGACTTGGAGAGTTTGAGANGNTGAGACTTTGNGTCGTTGANGTGATCAGTTTTGCGTCAAGCAAATTATGCTAAAGTCGTCNNNNNTTTNNNNNNNTTAAATACTAACTTCAAAGNTTCTAGATGGNNCAAAANGTTTNNNTTNNTTTNGTTTAGTNAACGNCGTTAACTAAACAAGTTCANTTAAGTTAAGTGATTAANTCAAAAGCACTTAACTTGAACTNAAACAAGTTTCTAAAGTAAAAAANATTACAAAGAGTTTTATGACATTAANCAATTATAAGACNNNNNNNNNNNNNNAATTTAANTNTAGATGATATAGAGAGTTTGCTNACANTACCTGGTTCAAAAACACGTCGANTCTTCTAGCACTTNTNATGATACTACCCTCCAATANATNNGTCATTTGTATAATCTCTACAAAGCATGCGCCCTTTCANANAGAAACAATCAAGATTCAAGAATACNAAATTTATNTTGAGCTCAAGATCCAGGANTTGACATTCAATNTGCATAATACAAAAGTTTTACTTAACAAGTACGAAANTTATTACTAAGGTTTTTATTTTTAAGCTTATAAGACATAAAGNAGAGCTCATAGTTTTAATATCTCCATGTTAGCCTAANTTATAAGACATANAGAGCATGACCTCCAAGTCTAACCACTTGGAGAAANTGAGAAACAAGTAAACAAATTAGGATTATAGGCATGGCCAAAGAAANGACAACAAGGAAAATGTTATTGAGATTCTTTNACCTCTTCATGTTNGATTGTCTNTGCGAGAGGAAGAACACTTGTTCAATCACACACTATTCAGNAAATCTTTCTCCTTGGTTTTCATTGTTCAATGCATTAACGGGTGAAGTTTCCCATCAGAAGTATTCACTAAACCNNNGCTTTTNTTTTGNACTTCCTTNGCANAGTNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=29
PRIMER_RIGHT_NUM_RETURNED=29
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=29
PRIMER_PAIR_0_PENALTY=6.115117
PRIMER_LEFT_0_PENALTY=5.872085
PRIMER_RIGHT_0_PENALTY=0.243031
PRIMER_LEFT_0_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_0_SEQUENCE=TTTGGCCATGCCTATAATCC
PRIMER_LEFT_0=841,21
PRIMER_RIGHT_0=1560,20
PRIMER_LEFT_0_TM=55.128
PRIMER_RIGHT_0_TM=59.757
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=720
PRIMER_PAIR_1_PENALTY=6.711037
PRIMER_LEFT_1_PENALTY=5.872085
PRIMER_RIGHT_1_PENALTY=0.838952
PRIMER_LEFT_1_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_1_SEQUENCE=CAAGTGTTCTTCCTCTCGCA
PRIMER_LEFT_1=841,21
PRIMER_RIGHT_1=1640,20
PRIMER_LEFT_1_TM=55.128
PRIMER_RIGHT_1_TM=59.161
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=800
PRIMER_PAIR_2_PENALTY=6.915176
PRIMER_LEFT_2_PENALTY=5.872085
PRIMER_RIGHT_2_PENALTY=1.043090
PRIMER_LEFT_2_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_2_SEQUENCE=ACAAGTGTTCTTCCTCTCGCA
PRIMER_LEFT_2=841,21
PRIMER_RIGHT_2=1641,21
PRIMER_LEFT_2_TM=55.128
PRIMER_RIGHT_2_TM=60.043
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=801
PRIMER_PAIR_3_PENALTY=6.986760
PRIMER_LEFT_3_PENALTY=5.872085
PRIMER_RIGHT_3_PENALTY=1.114674
PRIMER_LEFT_3_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_3_SEQUENCE=GATGGGAAACTTCACCCGTT
PRIMER_LEFT_3=841,21
PRIMER_RIGHT_3=1717,20
PRIMER_LEFT_3_TM=55.128
PRIMER_RIGHT_3_TM=61.115
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=877
PRIMER_PAIR_4_PENALTY=7.447855
PRIMER_LEFT_4_PENALTY=5.872085
PRIMER_RIGHT_4_PENALTY=1.575769
PRIMER_LEFT_4_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_4_SEQUENCE=TGGCCATGCCTATAATCCTAA
PRIMER_LEFT_4=841,21
PRIMER_RIGHT_4=1558,21
PRIMER_LEFT_4_TM=55.128
PRIMER_RIGHT_4_TM=59.424
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=718
PRIMER_PAIR_5_PENALTY=7.520818
PRIMER_LEFT_5_PENALTY=5.872085
PRIMER_RIGHT_5_PENALTY=1.648733
PRIMER_LEFT_5_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_5_SEQUENCE=CTTTGGCCATGCCTATAATCC
PRIMER_LEFT_5=841,21
PRIMER_RIGHT_5=1561,21
PRIMER_LEFT_5_TM=55.128
PRIMER_RIGHT_5_TM=60.649
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=721
PRIMER_PAIR_6_PENALTY=7.725264
PRIMER_LEFT_6_PENALTY=5.872085
PRIMER_RIGHT_6_PENALTY=1.853178
PRIMER_LEFT_6_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_6_SEQUENCE=GCGCATGCTTTGTAGAGATT
PRIMER_LEFT_6=841,21
PRIMER_RIGHT_6=1274,20
PRIMER_LEFT_6_TM=55.128
PRIMER_RIGHT_6_TM=58.147
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=2.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=434
PRIMER_PAIR_7_PENALTY=8.228678
PRIMER_LEFT_7_PENALTY=5.872085
PRIMER_RIGHT_7_PENALTY=2.356592
PRIMER_LEFT_7_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_7_SEQUENCE=GGTTAGACTTGGAGGTCATGCT
PRIMER_LEFT_7=841,21
PRIMER_RIGHT_7=1506,22
PRIMER_LEFT_7_TM=55.128
PRIMER_RIGHT_7_TM=59.643
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=666
PRIMER_PAIR_8_PENALTY=8.376927
PRIMER_LEFT_8_PENALTY=5.872085
PRIMER_RIGHT_8_PENALTY=2.504842
PRIMER_LEFT_8_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_8_SEQUENCE=AAAGGGCGCATGCTTTGTAG
PRIMER_LEFT_8=841,21
PRIMER_RIGHT_8=1279,20
PRIMER_LEFT_8_TM=55.128
PRIMER_RIGHT_8_TM=62.505
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=439
PRIMER_PAIR_9_PENALTY=8.498558
PRIMER_LEFT_9_PENALTY=5.872085
PRIMER_RIGHT_9_PENALTY=2.626472
PRIMER_LEFT_9_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_9_SEQUENCE=GGCGCATGCTTTGTAGAGATT
PRIMER_LEFT_9=841,21
PRIMER_RIGHT_9=1275,21
PRIMER_LEFT_9_TM=55.128
PRIMER_RIGHT_9_TM=61.626
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=2.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=435
PRIMER_PAIR_10_PENALTY=8.508313
PRIMER_LEFT_10_PENALTY=5.872085
PRIMER_RIGHT_10_PENALTY=2.636228
PRIMER_LEFT_10_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_10_SEQUENCE=TTGGCCATGCCTATAATCCTAA
PRIMER_LEFT_10=841,21
PRIMER_RIGHT_10=1559,22
PRIMER_LEFT_10_TM=55.128
PRIMER_RIGHT_10_TM=60.636
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=719
PRIMER_PAIR_11_PENALTY=9.135699
PRIMER_LEFT_11_PENALTY=5.872085
PRIMER_RIGHT_11_PENALTY=3.263613
PRIMER_LEFT_11_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_11_SEQUENCE=GGCCATGCCTATAATCCTAA
PRIMER_LEFT_11=841,21
PRIMER_RIGHT_11=1557,20
PRIMER_LEFT_11_TM=55.128
PRIMER_RIGHT_11_TM=56.736
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=717
PRIMER_PAIR_12_PENALTY=9.209907
PRIMER_LEFT_12_PENALTY=5.872085
PRIMER_RIGHT_12_PENALTY=3.337822
PRIMER_LEFT_12_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_12_SEQUENCE=AACAAGTGTTCTTCCTCTCGCA
PRIMER_LEFT_12=841,21
PRIMER_RIGHT_12=1642,22
PRIMER_LEFT_12_TM=55.128
PRIMER_RIGHT_12_TM=61.338
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.455
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=10.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=802
PRIMER_PAIR_13_PENALTY=10.152681
PRIMER_LEFT_13_PENALTY=5.872085
PRIMER_RIGHT_13_PENALTY=4.280596
PRIMER_LEFT_13_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_13_SEQUENCE=TCTTTGGCCATGCCTATAATCC
PRIMER_LEFT_13=841,21
PRIMER_RIGHT_13=1562,22
PRIMER_LEFT_13_TM=55.128
PRIMER_RIGHT_13_TM=62.281
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=722
PRIMER_PAIR_14_PENALTY=10.656724
PRIMER_LEFT_14_PENALTY=5.872085
PRIMER_RIGHT_14_PENALTY=4.784639
PRIMER_LEFT_14_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_14_SEQUENCE=TGATGGGAAACTTCACCCGTT
PRIMER_LEFT_14=841,21
PRIMER_RIGHT_14=1718,21
PRIMER_LEFT_14_TM=55.128
PRIMER_RIGHT_14_TM=63.785
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=878
PRIMER_PAIR_15_PENALTY=10.887664
PRIMER_LEFT_15_PENALTY=5.872085
PRIMER_RIGHT_15_PENALTY=5.015579
PRIMER_LEFT_15_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_15_SEQUENCE=GTTAGACTTGGAGGTCATGCT
PRIMER_LEFT_15=841,21
PRIMER_RIGHT_15=1505,21
PRIMER_LEFT_15_TM=55.128
PRIMER_RIGHT_15_TM=55.984
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=665
PRIMER_PAIR_16_PENALTY=11.006890
PRIMER_LEFT_16_PENALTY=5.872085
PRIMER_RIGHT_16_PENALTY=5.134805
PRIMER_LEFT_16_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_16_SEQUENCE=GAAAGGGCGCATGCTTTGTAG
PRIMER_LEFT_16=841,21
PRIMER_RIGHT_16=1280,21
PRIMER_LEFT_16_TM=55.128
PRIMER_RIGHT_16_TM=64.135
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=5.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=440
PRIMER_PAIR_17_PENALTY=11.132045
PRIMER_LEFT_17_PENALTY=5.872085
PRIMER_RIGHT_17_PENALTY=5.259960
PRIMER_LEFT_17_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_17_SEQUENCE=TGGTTAGACTTGGAGGTCATGCT
PRIMER_LEFT_17=841,21
PRIMER_RIGHT_17=1507,23
PRIMER_LEFT_17_TM=55.128
PRIMER_RIGHT_17_TM=62.260
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.1000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=667
PRIMER_PAIR_18_PENALTY=11.707668
PRIMER_LEFT_18_PENALTY=5.872085
PRIMER_RIGHT_18_PENALTY=5.835582
PRIMER_LEFT_18_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_18_SEQUENCE=GGTTTAGTGAATACTTCTGATGGG
PRIMER_LEFT_18=841,21
PRIMER_RIGHT_18=1735,24
PRIMER_LEFT_18_TM=55.128
PRIMER_RIGHT_18_TM=58.164
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=41.667
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=9.6000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=895
PRIMER_PAIR_19_PENALTY=12.183283
PRIMER_LEFT_19_PENALTY=5.872085
PRIMER_RIGHT_19_PENALTY=6.311198
PRIMER_LEFT_19_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_19_SEQUENCE=TTCTTTGGCCATGCCTATAATCC
PRIMER_LEFT_19=841,21
PRIMER_RIGHT_19=1563,23
PRIMER_LEFT_19_TM=55.128
PRIMER_RIGHT_19_TM=63.311
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=723
PRIMER_PAIR_20_PENALTY=12.321767
PRIMER_LEFT_20_PENALTY=5.872085
PRIMER_RIGHT_20_PENALTY=6.449681
PRIMER_LEFT_20_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_20_SEQUENCE=CTTTGGCCATGCCTATAATCCTAA
PRIMER_LEFT_20=841,21
PRIMER_RIGHT_20=1561,24
PRIMER_LEFT_20_TM=55.128
PRIMER_RIGHT_20_TM=62.450
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=41.667
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=7.5000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=721
PRIMER_PAIR_21_PENALTY=12.379751
PRIMER_LEFT_21_PENALTY=5.872085
PRIMER_RIGHT_21_PENALTY=6.507665
PRIMER_LEFT_21_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_21_SEQUENCE=CTGATGGGAAACTTCACCCGTT
PRIMER_LEFT_21=841,21
PRIMER_RIGHT_21=1719,22
PRIMER_LEFT_21_TM=55.128
PRIMER_RIGHT_21_TM=64.508
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=9.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=879
PRIMER_PAIR_22_PENALTY=12.553145
PRIMER_LEFT_22_PENALTY=5.872085
PRIMER_RIGHT_22_PENALTY=6.681060
PRIMER_LEFT_22_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_22_SEQUENCE=GTGAATACTTCTGATGGGAAAC
PRIMER_LEFT_22=841,21
PRIMER_RIGHT_22=1729,22
PRIMER_LEFT_22_TM=55.128
PRIMER_RIGHT_22_TM=55.319
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=889
PRIMER_PAIR_23_PENALTY=12.600540
PRIMER_LEFT_23_PENALTY=5.872085
PRIMER_RIGHT_23_PENALTY=6.728455
PRIMER_LEFT_23_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_23_SEQUENCE=GGGCGCATGCTTTGTAGAGATT
PRIMER_LEFT_23=841,21
PRIMER_RIGHT_23=1276,22
PRIMER_LEFT_23_TM=55.128
PRIMER_RIGHT_23_TM=64.728
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=436
PRIMER_PAIR_24_PENALTY=12.797217
PRIMER_LEFT_24_PENALTY=5.872085
PRIMER_RIGHT_24_PENALTY=6.925131
PRIMER_LEFT_24_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_24_SEQUENCE=GTGGTTAGACTTGGAGGTCATGCT
PRIMER_LEFT_24=841,21
PRIMER_RIGHT_24=1508,24
PRIMER_LEFT_24_TM=55.128
PRIMER_RIGHT_24_TM=62.925
PRIMER_LEFT_24_GC_PERCENT=42.857
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=8.1000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=668
PRIMER_PAIR_25_PENALTY=14.122200
PRIMER_LEFT_25_PENALTY=5.872085
PRIMER_RIGHT_25_PENALTY=8.250115
PRIMER_LEFT_25_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_25_SEQUENCE=TTTCTTTGGCCATGCCTATAATCC
PRIMER_LEFT_25=841,21
PRIMER_RIGHT_25=1564,24
PRIMER_LEFT_25_TM=55.128
PRIMER_RIGHT_25_TM=64.250
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=41.667
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=8.1000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=724
PRIMER_PAIR_26_PENALTY=14.507317
PRIMER_LEFT_26_PENALTY=5.872085
PRIMER_RIGHT_26_PENALTY=8.635232
PRIMER_LEFT_26_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_26_SEQUENCE=AGTGGTTAGACTTGGAGGTCATGCT
PRIMER_LEFT_26=841,21
PRIMER_RIGHT_26=1509,25
PRIMER_LEFT_26_TM=55.128
PRIMER_RIGHT_26_TM=63.635
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=48.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=669
PRIMER_PAIR_27_PENALTY=14.727698
PRIMER_LEFT_27_PENALTY=5.872085
PRIMER_RIGHT_27_PENALTY=8.855613
PRIMER_LEFT_27_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_27_SEQUENCE=TCTTTGGCCATGCCTATAATCCTAA
PRIMER_LEFT_27=841,21
PRIMER_RIGHT_27=1562,25
PRIMER_LEFT_27_TM=55.128
PRIMER_RIGHT_27_TM=63.856
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.5000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=722
PRIMER_PAIR_28_PENALTY=16.445735
PRIMER_LEFT_28_PENALTY=5.872085
PRIMER_RIGHT_28_PENALTY=10.573650
PRIMER_LEFT_28_SEQUENCE=TCAGACTTGGAGAGTTTGAGA
PRIMER_RIGHT_28_SEQUENCE=AAGTGGTTAGACTTGGAGGTCATGCT
PRIMER_LEFT_28=841,21
PRIMER_RIGHT_28=1510,26
PRIMER_LEFT_28_TM=55.128
PRIMER_RIGHT_28_TM=64.574
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=46.154
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=670
=
SEQUENCE_ID=MAGIC.175_3_11956787_11956866_sf-2_col-0_149213_78_TRUE
SEQUENCE_TARGET=950,179
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACGTTCTATAATTTNTATNNCTTTNAATAATTTATTNAATTAATACTTAAATAATAAAGGGTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTNAANNANATAAATGGTATGTCTTTCACTTTGNACGGAGTATCCCNATGTGGGGTACATAAATATTAAGAGAAAAAAAGAGATCAAGCTTACTAGTTTAAGCGTTAATAATTATACTGACTGACCAATCTACTTGTTCATGTAGCTCTAATGTTATTAATTANTATATAGTCTTAGATTGATCTAATTAATGTCTGTGTATGATGTCACTTTGTGATGATGGAAGTCATGAGAGCTTCCATTACTTAACTCTCTCTCTCTCTNTCTATNTATNTTTACGGTCATGTATAGTGTAATCANNAATAACAAGAAAAAGCCAAGTTCAAAGCACNATACTATTAGTTAAAGACAAATGCGATAATGATACGATTCTGAGCATTACTTGAGTCNCAATATATAGTCACACCCTCATCAAATTCTTTNTATCTTACCAACTTCTNAAAAAAAANNAAAAANNNAAAAGACAAGTAAATACATANATAAATATACACNCACACACACACACATATATTGATATACTTATAATNATAAATCATGGTAAAGTTTGANACAAAACTATGGAAGGCTGTGCTTATGATGTCCATGATCAACATTGGTTTAAGTGTTGTAAACGTTATGTTCAAGAAGATGATTGATGAAGGACTTAACCGTATGGTCGCGACCACTTACCGACTTGCTGTAGGAACTCTGTTCTTNATACCATTCGCGATTTTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNTTNNNNNNNNTNTNNNNNGNTNNNTGTNTTNTTNNTTNTNNNNNTTNNANTTNTNNTTNAANTNTNNNNTTNAATNATAANNGGATGTCTTGTTTATACNANTNTCTAGTTGAATGTTGTTTTCAAACTCTTTTCTCCTGTGAATATGTNATTATATATTCGTATTCCATATGAAAGATTTTGNNNNNNNNNNNNNTTGTTCTTTTTTTNNNNNTCTCTTTTNGTAGACATAACAGGCCAAAACTCACGGGTAGGATCTTGTGTTCACTCTTCTTTAGCGCTCTTCTGGGGTATGTTCATTTTATTAGACTGTTCANTTATAATAAATGTTTACAAATAAGTTTTGAGTTGTTTNTTNTGATCTTGATCGTTNNTATATTTGCAGGACAAGTTTGGTGCAATACTTCTTTCTTATTGGACTAGAGTATACATCTTCAACNTTTTCCTTAGCNTTTAGCAACATGGTTCCTTCNGTTACNTTTGCNTTGGCTCTCGTCTTTAGGTANTNAAAAATCGATCAAACNCATTTGNATTCTTCATATATATNCTCTAGTATTATCNNAATNNTTNATAAACTANANGANTAGGCAAGAGACGCTGAACATCAAGAGCAACGTAGGAAGAGCCAAACTGCTAGGCACAATGATATGTATTTGTGGAGCTTTAGTGCTTACGCTTTATAAAGGAACCGCTTTAAGTCGAGAACANAGTACTCATATGNAAACACATACAAGAACTGATTCCACCGGTGCAATGACNCAAAAGTGGGCAATGGGTTCGATCATGCTGGTTATATCNATCATNATATGGTCATCTTGGTTCATTGTTCAAGCTAAAATAAGCNGANTTTATCCNTGCCAATACACGAGTACCACGATCCTCTCTTTCTTTGGCGTGATNCAATCTGCTTTGTTGAGTTTGATCTCGGAGAGGAGCACNTCTATGTGGGTCGTTAAAGACAAGTTCCAAGTTTTAGCTTTACTTTATTCGGTGAGTNA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.689693
PRIMER_LEFT_0_PENALTY=1.674441
PRIMER_RIGHT_0_PENALTY=0.015253
PRIMER_LEFT_0_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_0_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_0=864,20
PRIMER_RIGHT_0=1709,20
PRIMER_LEFT_0_TM=58.326
PRIMER_RIGHT_0_TM=60.015
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.9000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=846
PRIMER_PAIR_1_PENALTY=1.694207
PRIMER_LEFT_1_PENALTY=1.674441
PRIMER_RIGHT_1_PENALTY=0.019767
PRIMER_LEFT_1_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_1_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_1=864,20
PRIMER_RIGHT_1=1685,20
PRIMER_LEFT_1_TM=58.326
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=822
PRIMER_PAIR_2_PENALTY=1.699569
PRIMER_LEFT_2_PENALTY=1.674441
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_2_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_2=864,20
PRIMER_RIGHT_2=1373,20
PRIMER_LEFT_2_TM=58.326
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=510
PRIMER_PAIR_3_PENALTY=1.708121
PRIMER_LEFT_3_PENALTY=1.674441
PRIMER_RIGHT_3_PENALTY=0.033680
PRIMER_LEFT_3_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_3_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_3=864,20
PRIMER_RIGHT_3=1829,20
PRIMER_LEFT_3_TM=58.326
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=966
PRIMER_PAIR_4_PENALTY=1.708121
PRIMER_LEFT_4_PENALTY=1.674441
PRIMER_RIGHT_4_PENALTY=0.033680
PRIMER_LEFT_4_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_4_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_4=864,20
PRIMER_RIGHT_4=1830,20
PRIMER_LEFT_4_TM=58.326
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=967
PRIMER_PAIR_5_PENALTY=1.802887
PRIMER_LEFT_5_PENALTY=1.787634
PRIMER_RIGHT_5_PENALTY=0.015253
PRIMER_LEFT_5_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_5_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_5=866,21
PRIMER_RIGHT_5=1709,20
PRIMER_LEFT_5_TM=59.212
PRIMER_RIGHT_5_TM=60.015
PRIMER_LEFT_5_GC_PERCENT=47.619
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=844
PRIMER_PAIR_6_PENALTY=1.802887
PRIMER_LEFT_6_PENALTY=1.787634
PRIMER_RIGHT_6_PENALTY=0.015253
PRIMER_LEFT_6_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_6_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_6=864,21
PRIMER_RIGHT_6=1709,20
PRIMER_LEFT_6_TM=59.212
PRIMER_RIGHT_6_TM=60.015
PRIMER_LEFT_6_GC_PERCENT=47.619
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.3000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=846
PRIMER_PAIR_7_PENALTY=1.807401
PRIMER_LEFT_7_PENALTY=1.787634
PRIMER_RIGHT_7_PENALTY=0.019767
PRIMER_LEFT_7_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_7_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_7=866,21
PRIMER_RIGHT_7=1685,20
PRIMER_LEFT_7_TM=59.212
PRIMER_RIGHT_7_TM=60.020
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=820
PRIMER_PAIR_8_PENALTY=1.807401
PRIMER_LEFT_8_PENALTY=1.787634
PRIMER_RIGHT_8_PENALTY=0.019767
PRIMER_LEFT_8_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_8_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_8=864,21
PRIMER_RIGHT_8=1685,20
PRIMER_LEFT_8_TM=59.212
PRIMER_RIGHT_8_TM=60.020
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.3000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=822
PRIMER_PAIR_9_PENALTY=1.812762
PRIMER_LEFT_9_PENALTY=1.787634
PRIMER_RIGHT_9_PENALTY=0.025128
PRIMER_LEFT_9_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_9_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_9=866,21
PRIMER_RIGHT_9=1373,20
PRIMER_LEFT_9_TM=59.212
PRIMER_RIGHT_9_TM=59.975
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=508
PRIMER_PAIR_10_PENALTY=1.812762
PRIMER_LEFT_10_PENALTY=1.787634
PRIMER_RIGHT_10_PENALTY=0.025128
PRIMER_LEFT_10_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_10_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_10=864,21
PRIMER_RIGHT_10=1373,20
PRIMER_LEFT_10_TM=59.212
PRIMER_RIGHT_10_TM=59.975
PRIMER_LEFT_10_GC_PERCENT=47.619
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.3000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=510
PRIMER_PAIR_11_PENALTY=1.821314
PRIMER_LEFT_11_PENALTY=1.787634
PRIMER_RIGHT_11_PENALTY=0.033680
PRIMER_LEFT_11_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_11_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_11=866,21
PRIMER_RIGHT_11=1829,20
PRIMER_LEFT_11_TM=59.212
PRIMER_RIGHT_11_TM=59.966
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=964
PRIMER_PAIR_12_PENALTY=1.821314
PRIMER_LEFT_12_PENALTY=1.787634
PRIMER_RIGHT_12_PENALTY=0.033680
PRIMER_LEFT_12_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_12_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_12=866,21
PRIMER_RIGHT_12=1830,20
PRIMER_LEFT_12_TM=59.212
PRIMER_RIGHT_12_TM=59.966
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=965
PRIMER_PAIR_13_PENALTY=1.821314
PRIMER_LEFT_13_PENALTY=1.787634
PRIMER_RIGHT_13_PENALTY=0.033680
PRIMER_LEFT_13_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_13_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_13=864,21
PRIMER_RIGHT_13=1829,20
PRIMER_LEFT_13_TM=59.212
PRIMER_RIGHT_13_TM=59.966
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.3000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=966
PRIMER_PAIR_14_PENALTY=1.821314
PRIMER_LEFT_14_PENALTY=1.787634
PRIMER_RIGHT_14_PENALTY=0.033680
PRIMER_LEFT_14_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_14_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_14=864,21
PRIMER_RIGHT_14=1830,20
PRIMER_LEFT_14_TM=59.212
PRIMER_RIGHT_14_TM=59.966
PRIMER_LEFT_14_GC_PERCENT=47.619
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.3000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=967
PRIMER_PAIR_15_PENALTY=1.828463
PRIMER_LEFT_15_PENALTY=1.674441
PRIMER_RIGHT_15_PENALTY=0.154023
PRIMER_LEFT_15_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_15_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_15=864,20
PRIMER_RIGHT_15=1720,20
PRIMER_LEFT_15_TM=58.326
PRIMER_RIGHT_15_TM=60.154
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=857
PRIMER_PAIR_16_PENALTY=1.940877
PRIMER_LEFT_16_PENALTY=1.674441
PRIMER_RIGHT_16_PENALTY=0.266437
PRIMER_LEFT_16_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_16_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_16=864,20
PRIMER_RIGHT_16=1478,20
PRIMER_LEFT_16_TM=58.326
PRIMER_RIGHT_16_TM=59.734
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=8.8000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=615
PRIMER_PAIR_17_PENALTY=1.941657
PRIMER_LEFT_17_PENALTY=1.787634
PRIMER_RIGHT_17_PENALTY=0.154023
PRIMER_LEFT_17_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_17_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_17=866,21
PRIMER_RIGHT_17=1720,20
PRIMER_LEFT_17_TM=59.212
PRIMER_RIGHT_17_TM=60.154
PRIMER_LEFT_17_GC_PERCENT=47.619
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=855
PRIMER_PAIR_18_PENALTY=1.941657
PRIMER_LEFT_18_PENALTY=1.787634
PRIMER_RIGHT_18_PENALTY=0.154023
PRIMER_LEFT_18_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_18_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_18=864,21
PRIMER_RIGHT_18=1720,20
PRIMER_LEFT_18_TM=59.212
PRIMER_RIGHT_18_TM=60.154
PRIMER_LEFT_18_GC_PERCENT=47.619
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.3000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=857
PRIMER_PAIR_19_PENALTY=1.950190
PRIMER_LEFT_19_PENALTY=1.674441
PRIMER_RIGHT_19_PENALTY=0.275749
PRIMER_LEFT_19_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_19_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_19=864,20
PRIMER_RIGHT_19=1370,20
PRIMER_LEFT_19_TM=58.326
PRIMER_RIGHT_19_TM=59.724
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=507
PRIMER_PAIR_20_PENALTY=2.030013
PRIMER_LEFT_20_PENALTY=1.674441
PRIMER_RIGHT_20_PENALTY=0.355573
PRIMER_LEFT_20_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_20_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_20=864,20
PRIMER_RIGHT_20=1721,20
PRIMER_LEFT_20_TM=58.326
PRIMER_RIGHT_20_TM=59.644
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=858
PRIMER_PAIR_21_PENALTY=2.054071
PRIMER_LEFT_21_PENALTY=1.787634
PRIMER_RIGHT_21_PENALTY=0.266437
PRIMER_LEFT_21_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_21_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_21=866,21
PRIMER_RIGHT_21=1478,20
PRIMER_LEFT_21_TM=59.212
PRIMER_RIGHT_21_TM=59.734
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=613
PRIMER_PAIR_22_PENALTY=2.054071
PRIMER_LEFT_22_PENALTY=1.787634
PRIMER_RIGHT_22_PENALTY=0.266437
PRIMER_LEFT_22_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_22_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_22=864,21
PRIMER_RIGHT_22=1478,20
PRIMER_LEFT_22_TM=59.212
PRIMER_RIGHT_22_TM=59.734
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.3000
PRIMER_RIGHT_22_END_STABILITY=8.8000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=615
PRIMER_PAIR_23_PENALTY=2.063383
PRIMER_LEFT_23_PENALTY=1.787634
PRIMER_RIGHT_23_PENALTY=0.275749
PRIMER_LEFT_23_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_23_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_23=866,21
PRIMER_RIGHT_23=1370,20
PRIMER_LEFT_23_TM=59.212
PRIMER_RIGHT_23_TM=59.724
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=505
PRIMER_PAIR_24_PENALTY=2.063383
PRIMER_LEFT_24_PENALTY=1.787634
PRIMER_RIGHT_24_PENALTY=0.275749
PRIMER_LEFT_24_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_24_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_24=864,21
PRIMER_RIGHT_24=1370,20
PRIMER_LEFT_24_TM=59.212
PRIMER_RIGHT_24_TM=59.724
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.3000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=507
PRIMER_PAIR_25_PENALTY=2.078114
PRIMER_LEFT_25_PENALTY=1.674441
PRIMER_RIGHT_25_PENALTY=0.403673
PRIMER_LEFT_25_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_25_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_25=864,20
PRIMER_RIGHT_25=1694,20
PRIMER_LEFT_25_TM=58.326
PRIMER_RIGHT_25_TM=59.596
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.9000
PRIMER_RIGHT_25_END_STABILITY=9.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=831
PRIMER_PAIR_26_PENALTY=2.143207
PRIMER_LEFT_26_PENALTY=1.787634
PRIMER_RIGHT_26_PENALTY=0.355573
PRIMER_LEFT_26_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_26_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_26=866,21
PRIMER_RIGHT_26=1721,20
PRIMER_LEFT_26_TM=59.212
PRIMER_RIGHT_26_TM=59.644
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=856
PRIMER_PAIR_27_PENALTY=2.143207
PRIMER_LEFT_27_PENALTY=1.787634
PRIMER_RIGHT_27_PENALTY=0.355573
PRIMER_LEFT_27_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_27_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_27=864,21
PRIMER_RIGHT_27=1721,20
PRIMER_LEFT_27_TM=59.212
PRIMER_RIGHT_27_TM=59.644
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.3000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=858
PRIMER_PAIR_28_PENALTY=2.187625
PRIMER_LEFT_28_PENALTY=2.172373
PRIMER_RIGHT_28_PENALTY=0.015253
PRIMER_LEFT_28_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_28_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_28=863,21
PRIMER_RIGHT_28=1709,20
PRIMER_LEFT_28_TM=61.172
PRIMER_RIGHT_28_TM=60.015
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=847
PRIMER_PAIR_29_PENALTY=2.191308
PRIMER_LEFT_29_PENALTY=1.787634
PRIMER_RIGHT_29_PENALTY=0.403673
PRIMER_LEFT_29_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_29_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_29=866,21
PRIMER_RIGHT_29=1694,20
PRIMER_LEFT_29_TM=59.212
PRIMER_RIGHT_29_TM=59.596
PRIMER_LEFT_29_GC_PERCENT=47.619
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=829
PRIMER_PAIR_30_PENALTY=2.191308
PRIMER_LEFT_30_PENALTY=1.787634
PRIMER_RIGHT_30_PENALTY=0.403673
PRIMER_LEFT_30_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_30_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_30=864,21
PRIMER_RIGHT_30=1694,20
PRIMER_LEFT_30_TM=59.212
PRIMER_RIGHT_30_TM=59.596
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.3000
PRIMER_RIGHT_30_END_STABILITY=9.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=831
PRIMER_PAIR_31_PENALTY=2.192139
PRIMER_LEFT_31_PENALTY=2.172373
PRIMER_RIGHT_31_PENALTY=0.019767
PRIMER_LEFT_31_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_31_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_31=863,21
PRIMER_RIGHT_31=1685,20
PRIMER_LEFT_31_TM=61.172
PRIMER_RIGHT_31_TM=60.020
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=823
PRIMER_PAIR_32_PENALTY=2.197501
PRIMER_LEFT_32_PENALTY=2.172373
PRIMER_RIGHT_32_PENALTY=0.025128
PRIMER_LEFT_32_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_32_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_32=863,21
PRIMER_RIGHT_32=1373,20
PRIMER_LEFT_32_TM=61.172
PRIMER_RIGHT_32_TM=59.975
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=6.3000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=511
PRIMER_PAIR_33_PENALTY=2.206053
PRIMER_LEFT_33_PENALTY=2.172373
PRIMER_RIGHT_33_PENALTY=0.033680
PRIMER_LEFT_33_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_33_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_33=863,21
PRIMER_RIGHT_33=1829,20
PRIMER_LEFT_33_TM=61.172
PRIMER_RIGHT_33_TM=59.966
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=967
PRIMER_PAIR_34_PENALTY=2.206053
PRIMER_LEFT_34_PENALTY=2.172373
PRIMER_RIGHT_34_PENALTY=0.033680
PRIMER_LEFT_34_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_34_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_34=863,21
PRIMER_RIGHT_34=1830,20
PRIMER_LEFT_34_TM=61.172
PRIMER_RIGHT_34_TM=59.966
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=968
PRIMER_PAIR_35_PENALTY=2.212524
PRIMER_LEFT_35_PENALTY=1.674441
PRIMER_RIGHT_35_PENALTY=0.538083
PRIMER_LEFT_35_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_35_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_35=864,20
PRIMER_RIGHT_35=1708,20
PRIMER_LEFT_35_TM=58.326
PRIMER_RIGHT_35_TM=60.538
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=845
PRIMER_PAIR_36_PENALTY=2.250680
PRIMER_LEFT_36_PENALTY=2.235428
PRIMER_RIGHT_36_PENALTY=0.015253
PRIMER_LEFT_36_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_36_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_36=867,20
PRIMER_RIGHT_36=1709,20
PRIMER_LEFT_36_TM=57.765
PRIMER_RIGHT_36_TM=60.015
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=8.5000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=843
PRIMER_PAIR_37_PENALTY=2.255194
PRIMER_LEFT_37_PENALTY=2.235428
PRIMER_RIGHT_37_PENALTY=0.019767
PRIMER_LEFT_37_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_37_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_37=867,20
PRIMER_RIGHT_37=1685,20
PRIMER_LEFT_37_TM=57.765
PRIMER_RIGHT_37_TM=60.020
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=819
PRIMER_PAIR_38_PENALTY=2.260556
PRIMER_LEFT_38_PENALTY=2.235428
PRIMER_RIGHT_38_PENALTY=0.025128
PRIMER_LEFT_38_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_38_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_38=867,20
PRIMER_RIGHT_38=1373,20
PRIMER_LEFT_38_TM=57.765
PRIMER_RIGHT_38_TM=59.975
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=507
PRIMER_PAIR_39_PENALTY=2.269108
PRIMER_LEFT_39_PENALTY=2.235428
PRIMER_RIGHT_39_PENALTY=0.033680
PRIMER_LEFT_39_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_39_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_39=867,20
PRIMER_RIGHT_39=1829,20
PRIMER_LEFT_39_TM=57.765
PRIMER_RIGHT_39_TM=59.966
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=963
PRIMER_PAIR_40_PENALTY=2.269108
PRIMER_LEFT_40_PENALTY=2.235428
PRIMER_RIGHT_40_PENALTY=0.033680
PRIMER_LEFT_40_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_40_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_40=867,20
PRIMER_RIGHT_40=1830,20
PRIMER_LEFT_40_TM=57.765
PRIMER_RIGHT_40_TM=59.966
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=964
PRIMER_PAIR_41_PENALTY=2.301346
PRIMER_LEFT_41_PENALTY=2.276218
PRIMER_RIGHT_41_PENALTY=0.025128
PRIMER_LEFT_41_SEQUENCE=GAAAAAGCCAAGTTCAAAGCAC
PRIMER_RIGHT_41_SEQUENCE=TACCCCAGAAGAGCGCTAAA
PRIMER_LEFT_41=614,22
PRIMER_RIGHT_41=1373,20
PRIMER_LEFT_41_TM=60.276
PRIMER_RIGHT_41_TM=59.975
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=6.3000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=760
PRIMER_PAIR_42_PENALTY=2.325718
PRIMER_LEFT_42_PENALTY=1.787634
PRIMER_RIGHT_42_PENALTY=0.538083
PRIMER_LEFT_42_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_42_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_42=866,21
PRIMER_RIGHT_42=1708,20
PRIMER_LEFT_42_TM=59.212
PRIMER_RIGHT_42_TM=60.538
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=843
PRIMER_PAIR_43_PENALTY=2.325718
PRIMER_LEFT_43_PENALTY=1.787634
PRIMER_RIGHT_43_PENALTY=0.538083
PRIMER_LEFT_43_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_43_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_43=864,21
PRIMER_RIGHT_43=1708,20
PRIMER_LEFT_43_TM=59.212
PRIMER_RIGHT_43_TM=60.538
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.3000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=845
PRIMER_PAIR_44_PENALTY=2.326395
PRIMER_LEFT_44_PENALTY=2.172373
PRIMER_RIGHT_44_PENALTY=0.154023
PRIMER_LEFT_44_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_44_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_44=863,21
PRIMER_RIGHT_44=1720,20
PRIMER_LEFT_44_TM=61.172
PRIMER_RIGHT_44_TM=60.154
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.9000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=858
PRIMER_PAIR_45_PENALTY=2.389450
PRIMER_LEFT_45_PENALTY=2.235428
PRIMER_RIGHT_45_PENALTY=0.154023
PRIMER_LEFT_45_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_45_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_45=867,20
PRIMER_RIGHT_45=1720,20
PRIMER_LEFT_45_TM=57.765
PRIMER_RIGHT_45_TM=60.154
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=854
PRIMER_PAIR_46_PENALTY=2.438809
PRIMER_LEFT_46_PENALTY=2.172373
PRIMER_RIGHT_46_PENALTY=0.266437
PRIMER_LEFT_46_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_46_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_46=863,21
PRIMER_RIGHT_46=1478,20
PRIMER_LEFT_46_TM=61.172
PRIMER_RIGHT_46_TM=59.734
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=8.8000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=616
PRIMER_PAIR_47_PENALTY=2.448122
PRIMER_LEFT_47_PENALTY=2.172373
PRIMER_RIGHT_47_PENALTY=0.275749
PRIMER_LEFT_47_SEQUENCE=TGGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_47_SEQUENCE=CCCAGAAGAGCGCTAAAGAA
PRIMER_LEFT_47=863,21
PRIMER_RIGHT_47=1370,20
PRIMER_LEFT_47_TM=61.172
PRIMER_RIGHT_47_TM=59.724
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.9000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=508
PRIMER_PAIR_48_PENALTY=2.452020
PRIMER_LEFT_48_PENALTY=1.674441
PRIMER_RIGHT_48_PENALTY=0.777580
PRIMER_LEFT_48_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_48_SEQUENCE=GCCTAGCAGTTTGGCTCTTC
PRIMER_LEFT_48=864,20
PRIMER_RIGHT_48=1719,20
PRIMER_LEFT_48_TM=58.326
PRIMER_RIGHT_48_TM=59.222
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=856
PRIMER_PAIR_49_PENALTY=2.501864
PRIMER_LEFT_49_PENALTY=2.235428
PRIMER_RIGHT_49_PENALTY=0.266437
PRIMER_LEFT_49_SEQUENCE=AGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_49_SEQUENCE=CACCAAACTTGTCCTGCAAA
PRIMER_LEFT_49=867,20
PRIMER_RIGHT_49=1478,20
PRIMER_LEFT_49_TM=57.765
PRIMER_RIGHT_49_TM=59.734
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=8.8000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=612
=
SEQUENCE_ID=MAGIC.287_4_4316342_4316418_wil-2_mt-0_22549_9664_TRUE
SEQUENCE_TARGET=950,176
SEQUENCE_TEMPLATE=CAGNTTNTTNGACAGAAAATACNGGAANAAACGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCCTAGTTGTGGGNTNGGAGTCTAATGAACTTTTTCTACAANTATTACATGAATTTAAAAGTAATATTTNTATAAAATTCANAAAGAGAAATGATTNNGTTTNCTACAGAAANAGATTTNGTACANCCTTTNGAANNTTTTCTTTAGTTNAGAATNGAAGTTTTGTGTAGATTTTGAAAACAAAAAGCNGTTNTTAGNGTTAAGAGNTTNTGGTTTAANAGCATCATTCTTGAGGGTTCCTNAGGTTGANTCTTAGGATAATTGAGTTAGAAATTTAATTNTTTTAATGANAATTTGNTAAGAGCGTCTCTAATATAAGAGACAATTAATNAGCTAAGAGCGGGATAGGTGTTNAAATTAGAGTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAACCCAATTTCTCACCGATTTCTCNCCGACTTNNTCGAAACCACAGAGTAAAACCNAATTNNCGAATCATCTNCAATCTGAAANTAAACTANTNNGCTNGAAAATTNTGNNAGNTGGGACTATNANGTTCCGTNGAGCTAGACCAATTCATCACAAGCGAGAAAGAAGGAGAANNANAGGAAATAGGAGAAGGGNTTTTTNGAAGCGATTNAGGCATTAGAGAGGATGANTAGAGAACCATCAATNTACGTTTAGTTTNAAAATCGTTAANGNTGTAGNAATCTCGANTGAAANNATTATTANTGTTTGACATAATATAAGAATTATACCCATAANTNTATATATACCTATAAAACTGCAAAGAANCANTTTTTCTAAATGATGGAAAANCNTGAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_11817450_11817620_wil-2_tsu-0_25065_3881_TRUE
SEQUENCE_TARGET=950,270
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCNTCACCAATATTTCTATTTATGAAGTATCACCCNCATCACTATTCTTNTTAATCTNATNCTCTAAAGNCTNAACAATATTAAAANTTNCNGTTCGTTNAACATACTGTAACATCGAATTANCGATTGACTTTGTTAGTGTTTCTNNTCNNNNNNNNNTNTTNTTTTTTCTCCTNNCTCACTTTTTATTNGTTTGNAAGACATNTTACTGANTAAAAAATAAATTTAAATTANNACAANCAAGAACNAACTNAGAGNNNNNNCATCATNTATATAANTGCATATGGTNGCANAAAANGTTAATACTATATNNTAGNAAATNACAACAAAAAGATNAATTTNATTACNAGGTNTATCNTCATTGGTANANTTNTTGGATTAATNANATNNATTNTAGAATATTTANGAGATANNNNCATANGTAAAATCAATGATTNNAAAAANAAGATGACAAATTTAGAACNATNNNANTANNGAACCACCNCAATGCTAGAGNAAGAAGAGNAATTAGAAAGAANGTGACNATGGATTGNGATTTAAGTTAATCTTNNNNNNNNNNNNNNNNNTTTCTAAAAANAGTNTTTTANTGGAGATAANTTTTTCTANTTAATNATTTANAATTAANATTTTTGAGTAAAAGNNAAAAAANANAATGACATCNAAAATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACATAATTTTGAANCCNAAAACGNTTTATATACACTATNGTTNGCAANCATGGACATGGNCTTGCCCNNCTTGTATTTGTGGNCNGCTNNGTTTGTAATANNNAAATCACACCTTACAATCCACNNGACNTAGATNTATTTNTCTTTNTACGCAAGATGAAGCTNTCNATTNTAGAACNGGGATTCTTGTGTTGTAAGGTGGTTCTTANTTTTATTAGCATTNAANNNNNNNNNNTTGATTGTGTATTATATNAANATNCGNCTNTTNTTTAAGAGACAAATGATGAGTTAAGAGNCGAGNACGTGTCTANNGNTAAGTGANGAAGANAAANTATAAAACCNAAAAAATCATGCNNGTGNNNCTCTCTCTNTTCCTCTTCNNTCTCTCNNNNTTTTTTGCNATTTNTATCCAATGAAAGCAAAGGNAATTTCTCTNNAAATCGNAACCAATCNGAGGATTNGANNATGTATATTCAATCATNNNNNNNNNNNNNNNNNCTTGNTCATCNAATCAGTNAGGTGGTCATTNAAAANNGGAGAGCAATCTCNCCAGATCTCTGAGTTTCGTANGATAAATCGNTGTTAATCGTTCACCAANCNGGTTGTCATGGTAANANTCTCTCTTAGTTTGTATTAGTTGNTGTTNATNGATTGTNTTNNAATANGGNTCATNAAAATGAGAAATTAGNNTTTTTTTNTTGTCTGNAAAATTAGGTTAATTTCNTGATTGNTTCGATTAATAGTCNGTTNTATCTTAATTTGATANTTACTANAGTCTTTGGTANTTAATGATTNTTTTGTGGGCANANAAAGTNTTTTTGAAGTNTTGATAANTTTTTNNATTAATAATCTTTGGATNTTAAATTTNTTCATGAATTTGATTAGTTATTNTTCCTANATNCATGAATGTTAGGTTTNTTNNNNNNNNNNNNNTTGAAACG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_11436932_11437331_oy-0_kn-0_1319644_223962_TRUE
SEQUENCE_TARGET=950,499
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4366886_4366929_sf-2_ler-0_48481_13467_TRUE
SEQUENCE_TARGET=950,143
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_4217517_4217522_po-0_ler-0_180513_51955_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGAGTGAGGCTCATCTATCACATGTTCTTTTTTGTNACACCTACTTTTTTTTGGTTACCATCAACCCACTTTTTTGTTGTTCATTCTTTTTTNTATATACCAAAACAACAAACTATAACGACTAAAAAATAAAANTATCACAATGAAAAGNANAAAANNNANATATTATCACAATTCACTATGACGTGAATACGAGCAAGTGCAATNCCATACAAGTGAAATTTAATTTTAAATATTTCATTACCTTTGTTCAANACAAGAACTATATATTTCATTGCCACCTTCGAGATANNNNNNNNNNNNNNNNNNNNNNTGTATAATAGACCTTTCATAAATCAATTTAAGACTAATTTGACTTAACTAGTGTTGTTTAGTAAGGTTNNNNTATGCCTAGTTGCTTATATTTTTAGGTAANAATATATGNNTTTTTTTCATTTTGCTTTGAGTTTTATGNTTTGTGGTTTTANTATNTTTTTTTTTTCTATATGGCTTAGACTCCANTNATTTTTTGTGTTCCTCCNGTTTTNGATTCCGAGTATTTATTCATNGCATANCGGCTTTNGTTTCTCNNTTTTTTTTTNTTNNNTTAGTCNNTCNCGTNTGTACTATATACTTNATTTCAACAAATTTATCAATATCAAATCAGTTGACAAAAACAAAGAATAATTTGATTTAACTTTGGTGTTTTTGTAATTATAAAATGAAATAAANGTGACGTNTGTAATGACAGATGACTTAGTTAGTCTATAGANCCGTGAAGCCATCGNCNTATTTTCAAGCCAAAGTCNCTTGAATTTATNANNTNGCTNTNNCNNTNTNTGANCANNNAANTANCNNTATCANNNAANATAATCATATGATGTGAANACCAAAAAATTAACATANTAAAAAGCTTATATGTTAAAACGAAGATATTTGACTACCAGCGACTTAATAAACTATATCCAAGTGAGATTGTGTGNNAAAAAATAACTANTNGTTATGTTTAGAATCAATTTCTTCGATGGGTTTTAGATAAATATTTTATTTAAGTAACTGCCATCCCAAACAACAAGGNGAAAATACAAATTGAAGGTGTTGTTTTCTAATAAAACAAACAATTNGTATTACAGAAATTCAAAATCAGTGGGAACCCCTCCAATTATTTTCCGACAATC
PRIMER_LEFT_NUM_RETURNED=16
PRIMER_RIGHT_NUM_RETURNED=16
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=16
PRIMER_PAIR_0_PENALTY=7.699206
PRIMER_LEFT_0_PENALTY=2.844757
PRIMER_RIGHT_0_PENALTY=4.854449
PRIMER_LEFT_0_SEQUENCE=GGTTACCATCAACCCACTTTTT
PRIMER_RIGHT_0_SEQUENCE=CGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_0=900,22
PRIMER_RIGHT_0=1775,24
PRIMER_LEFT_0_TM=59.155
PRIMER_RIGHT_0_TM=60.854
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=41.667
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=876
PRIMER_PAIR_1_PENALTY=8.027862
PRIMER_LEFT_1_PENALTY=3.173414
PRIMER_RIGHT_1_PENALTY=4.854449
PRIMER_LEFT_1_SEQUENCE=GGTTACCATCAACCCACTTTT
PRIMER_RIGHT_1_SEQUENCE=CGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_1=900,21
PRIMER_RIGHT_1=1775,24
PRIMER_LEFT_1_TM=57.827
PRIMER_RIGHT_1_TM=60.854
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=876
PRIMER_PAIR_2_PENALTY=8.504025
PRIMER_LEFT_2_PENALTY=3.649576
PRIMER_RIGHT_2_PENALTY=4.854449
PRIMER_LEFT_2_SEQUENCE=GGTTACCATCAACCCACTTT
PRIMER_RIGHT_2_SEQUENCE=CGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_2=900,20
PRIMER_RIGHT_2=1775,24
PRIMER_LEFT_2_TM=56.350
PRIMER_RIGHT_2_TM=60.854
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=876
PRIMER_PAIR_3_PENALTY=9.897872
PRIMER_LEFT_3_PENALTY=5.043423
PRIMER_RIGHT_3_PENALTY=4.854449
PRIMER_LEFT_3_SEQUENCE=GAGGCTCATCTATCACATGTTCTTT
PRIMER_RIGHT_3_SEQUENCE=CGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_3=853,25
PRIMER_RIGHT_3=1775,24
PRIMER_LEFT_3_TM=60.043
PRIMER_RIGHT_3_TM=60.854
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=923
PRIMER_PAIR_4_PENALTY=10.214448
PRIMER_LEFT_4_PENALTY=2.844757
PRIMER_RIGHT_4_PENALTY=7.369691
PRIMER_LEFT_4_SEQUENCE=GGTTACCATCAACCCACTTTTT
PRIMER_RIGHT_4_SEQUENCE=TCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_4=900,22
PRIMER_RIGHT_4=1776,25
PRIMER_LEFT_4_TM=59.155
PRIMER_RIGHT_4_TM=62.370
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=877
PRIMER_PAIR_5_PENALTY=10.543105
PRIMER_LEFT_5_PENALTY=3.173414
PRIMER_RIGHT_5_PENALTY=7.369691
PRIMER_LEFT_5_SEQUENCE=GGTTACCATCAACCCACTTTT
PRIMER_RIGHT_5_SEQUENCE=TCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_5=900,21
PRIMER_RIGHT_5=1776,25
PRIMER_LEFT_5_TM=57.827
PRIMER_RIGHT_5_TM=62.370
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.3000
PRIMER_RIGHT_5_END_STABILITY=8.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=877
PRIMER_PAIR_6_PENALTY=11.019268
PRIMER_LEFT_6_PENALTY=3.649576
PRIMER_RIGHT_6_PENALTY=7.369691
PRIMER_LEFT_6_SEQUENCE=GGTTACCATCAACCCACTTT
PRIMER_RIGHT_6_SEQUENCE=TCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_6=900,20
PRIMER_RIGHT_6=1776,25
PRIMER_LEFT_6_TM=56.350
PRIMER_RIGHT_6_TM=62.370
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=877
PRIMER_PAIR_7_PENALTY=11.805206
PRIMER_LEFT_7_PENALTY=2.844757
PRIMER_RIGHT_7_PENALTY=8.960449
PRIMER_LEFT_7_SEQUENCE=GGTTACCATCAACCCACTTTTT
PRIMER_RIGHT_7_SEQUENCE=GTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_7=900,22
PRIMER_RIGHT_7=1777,26
PRIMER_LEFT_7_TM=59.155
PRIMER_RIGHT_7_TM=62.960
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=42.308
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=878
PRIMER_PAIR_8_PENALTY=12.133863
PRIMER_LEFT_8_PENALTY=3.173414
PRIMER_RIGHT_8_PENALTY=8.960449
PRIMER_LEFT_8_SEQUENCE=GGTTACCATCAACCCACTTTT
PRIMER_RIGHT_8_SEQUENCE=GTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_8=900,21
PRIMER_RIGHT_8=1777,26
PRIMER_LEFT_8_TM=57.827
PRIMER_RIGHT_8_TM=62.960
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=42.308
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=878
PRIMER_PAIR_9_PENALTY=12.413115
PRIMER_LEFT_9_PENALTY=5.043423
PRIMER_RIGHT_9_PENALTY=7.369691
PRIMER_LEFT_9_SEQUENCE=GAGGCTCATCTATCACATGTTCTTT
PRIMER_RIGHT_9_SEQUENCE=TCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_9=853,25
PRIMER_RIGHT_9=1776,25
PRIMER_LEFT_9_TM=60.043
PRIMER_RIGHT_9_TM=62.370
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=924
PRIMER_PAIR_10_PENALTY=12.610026
PRIMER_LEFT_10_PENALTY=3.649576
PRIMER_RIGHT_10_PENALTY=8.960449
PRIMER_LEFT_10_SEQUENCE=GGTTACCATCAACCCACTTT
PRIMER_RIGHT_10_SEQUENCE=GTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_10=900,20
PRIMER_RIGHT_10=1777,26
PRIMER_LEFT_10_TM=56.350
PRIMER_RIGHT_10_TM=62.960
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=42.308
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=878
PRIMER_PAIR_11_PENALTY=13.441072
PRIMER_LEFT_11_PENALTY=2.844757
PRIMER_RIGHT_11_PENALTY=10.596315
PRIMER_LEFT_11_SEQUENCE=GGTTACCATCAACCCACTTTTT
PRIMER_RIGHT_11_SEQUENCE=AGTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_11=900,22
PRIMER_RIGHT_11=1778,27
PRIMER_LEFT_11_TM=59.155
PRIMER_RIGHT_11_TM=63.596
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=40.741
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=879
PRIMER_PAIR_12_PENALTY=13.769729
PRIMER_LEFT_12_PENALTY=3.173414
PRIMER_RIGHT_12_PENALTY=10.596315
PRIMER_LEFT_12_SEQUENCE=GGTTACCATCAACCCACTTTT
PRIMER_RIGHT_12_SEQUENCE=AGTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_12=900,21
PRIMER_RIGHT_12=1778,27
PRIMER_LEFT_12_TM=57.827
PRIMER_RIGHT_12_TM=63.596
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=40.741
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=879
PRIMER_PAIR_13_PENALTY=14.003873
PRIMER_LEFT_13_PENALTY=5.043423
PRIMER_RIGHT_13_PENALTY=8.960449
PRIMER_LEFT_13_SEQUENCE=GAGGCTCATCTATCACATGTTCTTT
PRIMER_RIGHT_13_SEQUENCE=GTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_13=853,25
PRIMER_RIGHT_13=1777,26
PRIMER_LEFT_13_TM=60.043
PRIMER_RIGHT_13_TM=62.960
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=42.308
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=925
PRIMER_PAIR_14_PENALTY=14.245892
PRIMER_LEFT_14_PENALTY=3.649576
PRIMER_RIGHT_14_PENALTY=10.596315
PRIMER_LEFT_14_SEQUENCE=GGTTACCATCAACCCACTTT
PRIMER_RIGHT_14_SEQUENCE=AGTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_14=900,20
PRIMER_RIGHT_14=1778,27
PRIMER_LEFT_14_TM=56.350
PRIMER_RIGHT_14_TM=63.596
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=40.741
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=879
PRIMER_PAIR_15_PENALTY=15.639739
PRIMER_LEFT_15_PENALTY=5.043423
PRIMER_RIGHT_15_PENALTY=10.596315
PRIMER_LEFT_15_SEQUENCE=GAGGCTCATCTATCACATGTTCTTT
PRIMER_RIGHT_15_SEQUENCE=AGTCGCTGGTAGTCAAATATCTTCGTT
PRIMER_LEFT_15=853,25
PRIMER_RIGHT_15=1778,27
PRIMER_LEFT_15_TM=60.043
PRIMER_RIGHT_15_TM=63.596
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.741
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=926
=
SEQUENCE_ID=MAGIC.338_5_8957502_8958011_wu-0_po-0_221664_604467_TRUE
SEQUENCE_TARGET=950,609
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCNTAAANGGAATTAAGTAATGNAGTTTGCNNNNNNNANGAGGATNTTGTGGAGATNTTTTCTTAAACAATTACGTCTAAGCAAGCAATGGAACTATGNAAGNAGTAAAGAAAGCAAAAGAAAGCGAGTAACCAAATGAAAATCGTAAGCAAACAACGAAACAAGTAGAGAAGTTCATGTAAGAAATAGTNNNGACGAAAGTAAAAAAGAAAAAGAAAAACCAGAGGATNAGGAAACAAGNAAACGAGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCGAGTATCCAAGAAGAAGGCTGCCCAATGAAGTAAAGTAAATCAAGGAACCAAGAAGGAAACAAACAAACAAGTCAAGCAACGATCAAATAATCAATGAAACAAAGAAGGAATAAAGTGAACAAGTTAAACATGTAAGCAATCAGTGCAAGAAACAANCAAGCACNTTACNAACTGAGATAAGCACAAGAAACAAACAAGAACAAGTTNTTNNNNNNNNNNNNNNNNNNNNAAGCANTGACCTAATCAATAAATCAAGAAAGCAAATATATATGCATGTGTATGNNNNNNNNNNNNNNNNNNNNNTATGNATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTATGCATGAATGCATGTATGGATGTATGCATGAATGCANCGNTATTTTGTTNTGGGANGCAGATTTTNCNNGTTCGNGAAACTGGATGTGGAAGAGGCTGATCAAGTACTCATCGNNNNNTGTGGGCTGACATAGATGTTGCTGTATCTCGTTTATCAGGTTGGTACTGGCACTTATCAATGATTGGATGANTCTGGTCCTCTAATTGATCTCACTAGCTCAAATGGTCTTCAAGTTTCTGGCATATCTCGGTTGGCAAAGTAGTGTCTCTCTCAGGTTTATCCTAATGGAACAAGAAGCAAACTGAGTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_6942612_6942695_can-0_bur-0_16876_127084_TRUE
SEQUENCE_TARGET=950,183
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTTTCTCTCTCCTCTGACGGTTCTTTTCTTCCTTCGTCCTCTCGTTTTTTTTGTTTCGTCGTCTCTCTNTCGCGCTCTGAAAANTTCGCAGAATCCGTCATGTCTTCCGGCGTAAATTCAACTGGCTCCGCCGCAGCAGCACCGGAGGTTGACAAGATGTTCTTCTGNTACCAGTGCAATCAAACAGTCACAATCTCAATTTCCTCATCCGCTGATCCTTTTTGTCCAATTTGTAACCAAGGGTTTCTTGAAGAATACGAAGACCCTAACCCTAATCAATCCCTCAATTTCAACCCTAACTCTTCTGATTCCTTTTTCCCCATGGCCGATCCTTTCTCCACCTTGCTCCCGCTCATATTCGGCTCTTCCGCTGCCGCTCCTTCCGGCATGGACTTCATGAGCTTATTCGGTCCTTCGATGCAACCACAGGCTCGTTCCACTCAGCAGAATCCTCAGTCTGACGCGTTTGATCCGTTTACGTTTCTTCAGAATCATCTCCAGACTTTGCGATCTAGCGGTACGCACTTTGAGTTCGTGATCGAGAATCATCCTTCGGATCCAGGTAATCGTATGCCTGGGAATTTCGGTGANTACTTCTTTGGTCCAGGTCTTGANCAGTTGATTCAGCAACTAGCTGAGAATGATCCTAATCGTTACGGAACTCCTCCTGCTTCCAAATCCGCCATTGATGCTCTTCCTACTGTTAAGGTAACGAAGGATATGTTGAAATCGGAGATGAACCAATGCGCNGTNTGTATGGATGAGTTTGANGATGGTAGCGATGTTAAGCAGATGCCTTGTAAGCACGTNTTTCATCAGGACTGTTTNCTCCCTTGGCTGGAGTTGCATAATTCGTGTCCTGTTTGTCGATTCGAGTTGCCTACGGATGATCCTGATTATGAGAACAGGAGTCAAGGAAGTCAGGGGAGTGGTGATGGACN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_12771597_12772094_ct-1_no-0_28552_40664_TRUE
SEQUENCE_TARGET=950,597
SEQUENCE_TEMPLATE=AGGCGAGAGGGTTATGTTTCNGCTGCAANNAACCNNNNNTATTCCCCTCAANATGATTGCCANTANAAGTCTCTTAGAGCATCCTCAANGGGAGGATTTAAGATNTTTNTTAACTAAATAAATNATTAAAATTAANTCCAAAATAATGACTTTAGGTTAGANGCGCTCTTAGCNTAGAGGCGCTCTTAGGTAATTGCAATGGTNATNTGGCATTGTTTGATGTCTTTACNTNNCTNCTNTCTCTCTCTCTCTCTNNNNNNNNNNNNGTCTCCCTCNCACCGGAGATGGATTCTTCANTATCTTCTCCTCAATCACAAATCCTTTATTGATCANACTATTCANNNNNNNNNNNNTACACGCTGCTCCTCTTCTTTTCCTTAGATTCCATGGCTTCACNNGAGAAATNNNNTCCTTGATAGTTAAGAACTGGAGATCTCTGTTATATCGATGAAGATGGATTCATATTTGTGGTTAATCGGTTAAANGAACTGATCAAGTACTATGTTAACATAGATTGTTACTTTAATTNCTTTAGTAAAGATTTATACTGGTTTTGGATGTATACTTTGTGANTNAAGNGAGANAANNNANTTTACAACTACNGTANNTGATTGATTTTTTAAGAACTCCANCTTANTATATTATCATTGNCAGANGNGTATCAAGGANTTTTTTTACCCAGTTCAAAACGTAACAAATAAAATGTCTTTAATNNAAAAAAATCNTTTTTTTACAACTTCTCAAACNAAAANAAACGTCCTACAAAAGCATCCTACGATCCTTTATGTCNTGNAATCTCTTTATCACAATCTCATTTGTAATANTATTGAATACATCTTTCTCAATAAAACAAACTAAACAATCATTCAAAAATTNNTCTCCAATACAATTTCGTCGATGTGTCTTTACGATCNTCATGGCTGAAAAACATCTCTCAACAGTAGCANNAGCAACANGCAAAATCAAAACCAATTTCAAAAGCCTATAACCAAANGGATATNACAAANGTTTTCGTGTTTCCACCANCTTCTGANCAATGTCTCCAAGATTCTTTAAATTAGAAAATCTCTCATCATTTCGAANAGTATCAATGTAGATAGATAATTNTTNCTCAATAGATATGAACTCTCCTAAACTAAAGTNANCTTGGTAGAACTTAGATAACCTCACNAACTTTGAGTGATCAAACTCANAAAATGCATCAATGGGAGNTAAAGAAGCANCACAAATAAGCAATTCAGTGTTCACCTCATTAAAANGGTCGTCAAATTTCTGAAGNTNGAAATCCAAAACANAATATAAGCAATTGACCTTGTAGTGATGCATATTTGATACTCTTNTTTTGTTTCTTCGTCTCTTTGAGTCAACAAAATCTTCCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAAACNAANAAACTTTCANNATGAGAGAATCTCACCCATCATCCCTAAAACACTGAAGTTGTCTCTTAGTTGATTTCACCAATGANATNGCATTNAAAATATNNTGATCTCTTCTTTNGAGNNCCATAGACAAGTTCTNTGTGAGCNCTANAANGTGTATCATCATGTGNNNNNNNNNNAANNNNTCAAAGNTATGAAAGNANNTNAGGAGACTATGTGCTTGNCGTCTNNNNNNGTNATCTANGCCGTAATTCTCAAAGCATTCNAGNACCTTGATGATAGAANAGTACANCTNNNNNNNNNNNNNNNNNNNNNNNNNNTGAGAATTCNAACGAGTANNTNCTGNTCNTTGANGAGANANCTCTTGATTNAACCNTNTTNTAGTACTGANTTCANCNNNNATNNNNCCNNCTNNNNNNNNNNNNNNNNNNNNNNNTCGGATCAAATNTTTCCNCTTGCATGAAGCTCNAACNACATTTAACAACAAAGAAATCANATNNAAAAAGTCTCCAACATTNANATGNTTTTNTNCAACTNNNNNNACAACTAANTGAANNTNNNNNNNNNNNCAATGAACATAANATGNTGATGTGNTNTCCTTCAAAATTAATGANTNNNNNNNNNNNNNNNNNNNNTTCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATCTCTCTTTGACTATCNCNNTTTTATCNNNAAANCTANNNNNNNNNNNCATTTGTTCTTTATCANACACATCAGCAGATTNATCTACNAACAAANCANNNNNGNNNNNNNNNNTTTCTTCAATTACAGATTTGNNNTCTGNAAAANAATGCANANATCTTTCTNNATCATAGGAGACTTAATTTNGTTATTTTCAGGAGCATTATCTAAAATAACTTTANNCACAACTTCANTTTGATCAGCAGTATACTTTAAGAGTTCCACAAAATTTCCTNTATTAGCANNATTTTNAGANTTATNATGNCCACGGAAANCTA
PRIMER_LEFT_NUM_RETURNED=12
PRIMER_RIGHT_NUM_RETURNED=12
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=12
PRIMER_PAIR_0_PENALTY=7.185844
PRIMER_LEFT_0_PENALTY=2.243575
PRIMER_RIGHT_0_PENALTY=4.942270
PRIMER_LEFT_0_SEQUENCE=CCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_0_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_0=880,22
PRIMER_RIGHT_0=1676,20
PRIMER_LEFT_0_TM=60.244
PRIMER_RIGHT_0_TM=55.058
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=8.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=797
PRIMER_PAIR_1_PENALTY=8.692931
PRIMER_LEFT_1_PENALTY=3.750661
PRIMER_RIGHT_1_PENALTY=4.942270
PRIMER_LEFT_1_SEQUENCE=CCCAGTTCAAAACGTAACAA
PRIMER_RIGHT_1_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_1=677,20
PRIMER_RIGHT_1=1676,20
PRIMER_LEFT_1_TM=56.249
PRIMER_RIGHT_1_TM=55.058
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=1000
PRIMER_PAIR_2_PENALTY=8.699246
PRIMER_LEFT_2_PENALTY=3.756976
PRIMER_RIGHT_2_PENALTY=4.942270
PRIMER_LEFT_2_SEQUENCE=TCATGGCTGAAAAACATCTC
PRIMER_RIGHT_2_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_2=914,20
PRIMER_RIGHT_2=1676,20
PRIMER_LEFT_2_TM=56.243
PRIMER_RIGHT_2_TM=55.058
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=763
PRIMER_PAIR_3_PENALTY=8.755513
PRIMER_LEFT_3_PENALTY=3.813243
PRIMER_RIGHT_3_PENALTY=4.942270
PRIMER_LEFT_3_SEQUENCE=CAATTTCGTCGATGTGTCTT
PRIMER_RIGHT_3_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_3=886,20
PRIMER_RIGHT_3=1676,20
PRIMER_LEFT_3_TM=56.187
PRIMER_RIGHT_3_TM=55.058
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=791
PRIMER_PAIR_4_PENALTY=9.223566
PRIMER_LEFT_4_PENALTY=4.281296
PRIMER_RIGHT_4_PENALTY=4.942270
PRIMER_LEFT_4_SEQUENCE=GCATCCTACGATCCTTTATGTC
PRIMER_RIGHT_4_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_4=767,22
PRIMER_RIGHT_4=1676,20
PRIMER_LEFT_4_TM=57.719
PRIMER_RIGHT_4_TM=55.058
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=910
PRIMER_PAIR_5_PENALTY=10.091304
PRIMER_LEFT_5_PENALTY=5.149034
PRIMER_RIGHT_5_PENALTY=4.942270
PRIMER_LEFT_5_SEQUENCE=GCATCCTACGATCCTTTATGT
PRIMER_RIGHT_5_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_5=767,21
PRIMER_RIGHT_5=1676,20
PRIMER_LEFT_5_TM=55.851
PRIMER_RIGHT_5_TM=55.058
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=5.6000
PRIMER_RIGHT_5_END_STABILITY=8.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=910
PRIMER_PAIR_6_PENALTY=11.573045
PRIMER_LEFT_6_PENALTY=6.630775
PRIMER_RIGHT_6_PENALTY=4.942270
PRIMER_LEFT_6_SEQUENCE=CCAATACAATTTCGTCGATGTGTC
PRIMER_RIGHT_6_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_6=880,24
PRIMER_RIGHT_6=1676,20
PRIMER_LEFT_6_TM=62.631
PRIMER_RIGHT_6_TM=55.058
PRIMER_LEFT_6_GC_PERCENT=41.667
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.1000
PRIMER_RIGHT_6_END_STABILITY=8.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=797
PRIMER_PAIR_7_PENALTY=11.672988
PRIMER_LEFT_7_PENALTY=6.730718
PRIMER_RIGHT_7_PENALTY=4.942270
PRIMER_LEFT_7_SEQUENCE=CTCCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_7_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_7=878,24
PRIMER_RIGHT_7=1676,20
PRIMER_LEFT_7_TM=62.731
PRIMER_RIGHT_7_TM=55.058
PRIMER_LEFT_7_GC_PERCENT=41.667
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=8.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=799
PRIMER_PAIR_8_PENALTY=14.117484
PRIMER_LEFT_8_PENALTY=9.175214
PRIMER_RIGHT_8_PENALTY=4.942270
PRIMER_LEFT_8_SEQUENCE=TCCAATACAATTTCGTCGATGTGTC
PRIMER_RIGHT_8_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_8=879,25
PRIMER_RIGHT_8=1676,20
PRIMER_LEFT_8_TM=64.175
PRIMER_RIGHT_8_TM=55.058
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=8.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=798
PRIMER_PAIR_9_PENALTY=14.204066
PRIMER_LEFT_9_PENALTY=9.261796
PRIMER_RIGHT_9_PENALTY=4.942270
PRIMER_LEFT_9_SEQUENCE=TCTCCAATACAATTTCGTCGATGTG
PRIMER_RIGHT_9_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_9=877,25
PRIMER_RIGHT_9=1676,20
PRIMER_LEFT_9_TM=64.262
PRIMER_RIGHT_9_TM=55.058
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=8.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=800
PRIMER_PAIR_10_PENALTY=15.757076
PRIMER_LEFT_10_PENALTY=10.814806
PRIMER_RIGHT_10_PENALTY=4.942270
PRIMER_LEFT_10_SEQUENCE=CTCCAATACAATTTCGTCGATGTGTC
PRIMER_RIGHT_10_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_10=878,26
PRIMER_RIGHT_10=1676,20
PRIMER_LEFT_10_TM=64.815
PRIMER_RIGHT_10_TM=55.058
PRIMER_LEFT_10_GC_PERCENT=42.308
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.1000
PRIMER_RIGHT_10_END_STABILITY=8.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=799
PRIMER_PAIR_11_PENALTY=16.072863
PRIMER_LEFT_11_PENALTY=11.130593
PRIMER_RIGHT_11_PENALTY=4.942270
PRIMER_LEFT_11_SEQUENCE=GCTGAAAAACATCTCTCAACAGTAGCA
PRIMER_RIGHT_11_SEQUENCE=GAATGCTTTGAGAATTACGG
PRIMER_LEFT_11=919,27
PRIMER_RIGHT_11=1676,20
PRIMER_LEFT_11_TM=64.131
PRIMER_RIGHT_11_TM=55.058
PRIMER_LEFT_11_GC_PERCENT=40.741
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.5000
PRIMER_RIGHT_11_END_STABILITY=8.9000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=758
=
SEQUENCE_ID=MAGIC.105_4_12127037_12127465_wu-0_hi-0_8097_25684_TRUE
SEQUENCE_TARGET=950,528
SEQUENCE_TEMPLATE=AACAACACNTTTAGNAGGTGTCCGAATNAGAGAGAAGCNGTGTTCTATTACGAGNAGTGCATACTNAGATACTCTCACAAGAATATTCTATCGACGGCCATTACAAACGAAGGAGAATTTATNNNNNNNNNNNNNNNNNNTATTTCTCCTATTCAAAATCAAATAAACCAGTTTACTAATNTGGTGTTATCTAATATGAACCAANNTGCCATCGAAGCAGCCGACANTNCTAGAAAATTCTCTACGATAAAGANCGAATNGNCCGCACTCCAGACTTTNTACGGGCTTGTTCAATGCACTCCTGATCTTTCAAGACAANACTGCATGAACTGTCTGACAAGTTCCATCAATAGAATGCCANTTNNTAGAANTGGAGCAAGACANTTTTGGCCAAGTTGTAATTCAAGGTACGAGCTTTACGATTTCTACAACGAAACCGCCNTTGGTACACCACCACCGCCGCTGCCTCCGTTGGCATCTCCTTCACTANCTGGTGAACAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGCAGTATTACCAATATGGAANGCANANTTTTCAATTTTTGTTTNGTTATNAGTCTACTATTTTCTTACTAAAGCANATGATCTGTTTAAACGCAGATAAAAGTGGGAATTCAAATGTGGTNGTGGTAGCCGTTGTTGTGCCTNTCATAGTCGNTGTTCTGATTTTNATAGCTGGTTATTGTTTCNTTGCAAANAGGGNNNNNNNNNCTTATGGCACAACACCTNCTTTAGATGGTAAAGATTCAAGNCCTTAATTGGTTTGATTAATGTTTTTGACTAATATNTTTTNTCTTTCTTTTTCTGATTACTAAGACATTTATTTTGCNACAGAAGANGATAAAACAACCATAGAGTCGCTGCNACTTGATTATAGAGCAATTCAAGCTGCAACAAATGATTTTTCAGAGAATAATAAAATTGGTCGAGGAGGNTTTGGTGACGTTTACAAGGTATAATATTATATACTNNTACATAANTTATCGATTGTNNNNTTTTNTTTTTGCTACAACACATTTGGATAATGAGATTGNTAAAAATGGATAGGGTACATTTTCAAATGGAACCGAAGTTGCAGTGAAGAGACTGTCGAAAACATCAGAACAAGGTGACACAGAATTCAAGAACGAGGTTGTAGTTGTTGCAAATCTTCGGCACAAAAATCTNGTTAGGATTCTNGGATTTTCTATAGAACGAGAAGAAAGGATATTGGTCTACGNGTATGTAGAAAATAAAAGCCTTGATAACTTCCTATTTGGTTAGTTGCATTTGATGANTTNTTTCANNNATTCGTTAACCAGTCCTGAAACATGGTTAGAGCTAACTACTATTCATGGATGTTCACAGATCCTGCAAAGAAAGGTCAGTTGTACTGGACACAGCGATACCATATCATTGNTGGGATTGCTAGAGGGATCCTATATCTTCATCAAGATTCACGNCTCACAATCATACACCGTGACCTNAAAGCGAGTAACATTCTCCTNGATGCNGATATGAATCCTAAAATTGCTGATTTTGGAATGGCAAGGATCTTTGGAATGGATCAAACCCAGCAGAACACAAGCAGAATAGTTGGTACCTAGTAAGTCTTCCACTTGACTACTCTCTCACATGTTGAACATTTTATTTTGATAACATATTAAAGTTTTTGNTGTGGATATCCAGCGGTTACATGTCTCCTGAATATGCGATGCGTGGCCAGTTCTCAATGAAATCTGATGTCTATAGTTTCGGAGTGTTAGTTCTTGAGATTATAAGCGGTAGGAAGAACAACAGCTTTANCGAGACAGATGACGCACANGACTTGGTGACACATGTAAGTTTAAAGGAAAGGAGTNNTATATATNTACCTCTCCTCATCCTACAATAAGCAATANTTGCAATAACTGTTTGACTGATTGATTCTAGGCTTGGAGGCTTTGGAGAAACGGAACAGCGTTAGACCTCGTGGATCCATTCATTGCAGATAGTTGCCGGAAGAGTGAAGTGGTTCGATGCANCCATATCGGTCTTTTATGTGTTCAAGAAGATCCTGTAAAACGTCCAGCCATGTCAACCATTTCCGTGATGCTCACTAGTAATACAATGGCTTTACCAGCGCCTCAGCAACCAGGGTTTTTTGTTAGGAGTAGACCTGGAACAAACCGGCTTGATTCAGATCAATCAACGACCAACAAGTCTGTTACAGTATCTATTGACGATNNNTCNATGTCTGATTTAGATCCTCGTTGAATATGGTGTTATTANANCTTACGATTGAAATCAAGTTTAATTAAGCCTATATTTACATATTTCTAAACTTGATGAACCATGTTGATCCTTTAGTTTNGATTTGGTTTNNTNTTTNTTTNCAAGAATAATCAATTTAGTCTCAGCTATCAGCATCACNACCGAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.440449
PRIMER_LEFT_0_PENALTY=0.001278
PRIMER_RIGHT_0_PENALTY=0.439172
PRIMER_LEFT_0_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_0_SEQUENCE=CCTTGCCATTCCAAAATCAG
PRIMER_LEFT_0=908,20
PRIMER_RIGHT_0=1561,20
PRIMER_LEFT_0_TM=59.999
PRIMER_RIGHT_0_TM=60.439
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=654
PRIMER_PAIR_1_PENALTY=0.462143
PRIMER_LEFT_1_PENALTY=0.001278
PRIMER_RIGHT_1_PENALTY=0.460865
PRIMER_LEFT_1_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_1_SEQUENCE=TCTGCTGGGTTTGATCCATT
PRIMER_LEFT_1=908,20
PRIMER_RIGHT_1=1589,20
PRIMER_LEFT_1_TM=59.999
PRIMER_RIGHT_1_TM=60.461
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=682
PRIMER_PAIR_2_PENALTY=0.496625
PRIMER_LEFT_2_PENALTY=0.001278
PRIMER_RIGHT_2_PENALTY=0.495347
PRIMER_LEFT_2_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_2_SEQUENCE=AGATCCTTGCCATTCCAAAA
PRIMER_LEFT_2=908,20
PRIMER_RIGHT_2=1565,20
PRIMER_LEFT_2_TM=59.999
PRIMER_RIGHT_2_TM=59.505
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=658
PRIMER_PAIR_3_PENALTY=0.824936
PRIMER_LEFT_3_PENALTY=0.385764
PRIMER_RIGHT_3_PENALTY=0.439172
PRIMER_LEFT_3_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_3_SEQUENCE=CCTTGCCATTCCAAAATCAG
PRIMER_LEFT_3=907,20
PRIMER_RIGHT_3=1561,20
PRIMER_LEFT_3_TM=59.614
PRIMER_RIGHT_3_TM=60.439
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=655
PRIMER_PAIR_4_PENALTY=0.846630
PRIMER_LEFT_4_PENALTY=0.385764
PRIMER_RIGHT_4_PENALTY=0.460865
PRIMER_LEFT_4_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_4_SEQUENCE=TCTGCTGGGTTTGATCCATT
PRIMER_LEFT_4=907,20
PRIMER_RIGHT_4=1589,20
PRIMER_LEFT_4_TM=59.614
PRIMER_RIGHT_4_TM=60.461
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=683
PRIMER_PAIR_5_PENALTY=0.881111
PRIMER_LEFT_5_PENALTY=0.385764
PRIMER_RIGHT_5_PENALTY=0.495347
PRIMER_LEFT_5_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_5_SEQUENCE=AGATCCTTGCCATTCCAAAA
PRIMER_LEFT_5=907,20
PRIMER_RIGHT_5=1565,20
PRIMER_LEFT_5_TM=59.614
PRIMER_RIGHT_5_TM=59.505
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=659
PRIMER_PAIR_6_PENALTY=1.067848
PRIMER_LEFT_6_PENALTY=0.001278
PRIMER_RIGHT_6_PENALTY=1.066570
PRIMER_LEFT_6_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_6_SEQUENCE=GGAGACATGTAACCGCTGGA
PRIMER_LEFT_6=908,20
PRIMER_RIGHT_6=1712,20
PRIMER_LEFT_6_TM=59.999
PRIMER_RIGHT_6_TM=61.067
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=805
PRIMER_PAIR_7_PENALTY=1.166454
PRIMER_LEFT_7_PENALTY=0.001278
PRIMER_RIGHT_7_PENALTY=1.165177
PRIMER_LEFT_7_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_7_SEQUENCE=ATCCTTGCCATTCCAAAATC
PRIMER_LEFT_7=908,20
PRIMER_RIGHT_7=1563,20
PRIMER_LEFT_7_TM=59.999
PRIMER_RIGHT_7_TM=58.835
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=656
PRIMER_PAIR_8_PENALTY=1.345326
PRIMER_LEFT_8_PENALTY=0.001278
PRIMER_RIGHT_8_PENALTY=1.344048
PRIMER_LEFT_8_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_8_SEQUENCE=TCCTTGCCATTCCAAAATCA
PRIMER_LEFT_8=908,20
PRIMER_RIGHT_8=1562,20
PRIMER_LEFT_8_TM=59.999
PRIMER_RIGHT_8_TM=61.344
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=655
PRIMER_PAIR_9_PENALTY=1.450900
PRIMER_LEFT_9_PENALTY=0.001278
PRIMER_RIGHT_9_PENALTY=1.449622
PRIMER_LEFT_9_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_9_SEQUENCE=CTGCTTGTGTTCTGCTGGGT
PRIMER_LEFT_9=908,20
PRIMER_RIGHT_9=1599,20
PRIMER_LEFT_9_TM=59.999
PRIMER_RIGHT_9_TM=61.450
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=692
PRIMER_PAIR_10_PENALTY=1.550941
PRIMER_LEFT_10_PENALTY=0.385764
PRIMER_RIGHT_10_PENALTY=1.165177
PRIMER_LEFT_10_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_10_SEQUENCE=ATCCTTGCCATTCCAAAATC
PRIMER_LEFT_10=907,20
PRIMER_RIGHT_10=1563,20
PRIMER_LEFT_10_TM=59.614
PRIMER_RIGHT_10_TM=58.835
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=657
PRIMER_PAIR_11_PENALTY=1.729812
PRIMER_LEFT_11_PENALTY=0.385764
PRIMER_RIGHT_11_PENALTY=1.344048
PRIMER_LEFT_11_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_11_SEQUENCE=TCCTTGCCATTCCAAAATCA
PRIMER_LEFT_11=907,20
PRIMER_RIGHT_11=1562,20
PRIMER_LEFT_11_TM=59.614
PRIMER_RIGHT_11_TM=61.344
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=656
PRIMER_PAIR_12_PENALTY=1.730123
PRIMER_LEFT_12_PENALTY=0.001278
PRIMER_RIGHT_12_PENALTY=1.728845
PRIMER_LEFT_12_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_12_SEQUENCE=GCATCGCATATTCAGGAGAC
PRIMER_LEFT_12=908,20
PRIMER_RIGHT_12=1726,20
PRIMER_LEFT_12_TM=59.999
PRIMER_RIGHT_12_TM=58.271
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.1000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=819
PRIMER_PAIR_13_PENALTY=1.835387
PRIMER_LEFT_13_PENALTY=0.385764
PRIMER_RIGHT_13_PENALTY=1.449622
PRIMER_LEFT_13_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_13_SEQUENCE=CTGCTTGTGTTCTGCTGGGT
PRIMER_LEFT_13=907,20
PRIMER_RIGHT_13=1599,20
PRIMER_LEFT_13_TM=59.614
PRIMER_RIGHT_13_TM=61.450
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=693
PRIMER_PAIR_14_PENALTY=2.039053
PRIMER_LEFT_14_PENALTY=0.001278
PRIMER_RIGHT_14_PENALTY=2.037776
PRIMER_LEFT_14_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_14_SEQUENCE=GGGTTTGATCCATTCCAAAGA
PRIMER_LEFT_14=908,20
PRIMER_RIGHT_14=1583,21
PRIMER_LEFT_14_TM=59.999
PRIMER_RIGHT_14_TM=61.038
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=676
PRIMER_PAIR_15_PENALTY=2.114609
PRIMER_LEFT_15_PENALTY=0.385764
PRIMER_RIGHT_15_PENALTY=1.728845
PRIMER_LEFT_15_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_15_SEQUENCE=GCATCGCATATTCAGGAGAC
PRIMER_LEFT_15=907,20
PRIMER_RIGHT_15=1726,20
PRIMER_LEFT_15_TM=59.614
PRIMER_RIGHT_15_TM=58.271
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.1000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=820
PRIMER_PAIR_16_PENALTY=2.157787
PRIMER_LEFT_16_PENALTY=0.001278
PRIMER_RIGHT_16_PENALTY=2.156509
PRIMER_LEFT_16_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_16_SEQUENCE=GCATCGCATATTCAGGAGACA
PRIMER_LEFT_16=908,20
PRIMER_RIGHT_16=1726,21
PRIMER_LEFT_16_TM=59.999
PRIMER_RIGHT_16_TM=61.157
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=819
PRIMER_PAIR_17_PENALTY=2.215325
PRIMER_LEFT_17_PENALTY=0.001278
PRIMER_RIGHT_17_PENALTY=2.214047
PRIMER_LEFT_17_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_17_SEQUENCE=CGCATCGCATATTCAGGAGA
PRIMER_LEFT_17=908,20
PRIMER_RIGHT_17=1727,20
PRIMER_LEFT_17_TM=59.999
PRIMER_RIGHT_17_TM=62.214
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=820
PRIMER_PAIR_18_PENALTY=2.423540
PRIMER_LEFT_18_PENALTY=0.385764
PRIMER_RIGHT_18_PENALTY=2.037776
PRIMER_LEFT_18_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_18_SEQUENCE=GGGTTTGATCCATTCCAAAGA
PRIMER_LEFT_18=907,20
PRIMER_RIGHT_18=1583,21
PRIMER_LEFT_18_TM=59.614
PRIMER_RIGHT_18_TM=61.038
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=677
PRIMER_PAIR_19_PENALTY=2.447681
PRIMER_LEFT_19_PENALTY=0.001278
PRIMER_RIGHT_19_PENALTY=2.446403
PRIMER_LEFT_19_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_19_SEQUENCE=TGCCATTCCAAAATCAGCAA
PRIMER_LEFT_19=908,20
PRIMER_RIGHT_19=1558,20
PRIMER_LEFT_19_TM=59.999
PRIMER_RIGHT_19_TM=62.446
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=651
PRIMER_PAIR_20_PENALTY=2.477995
PRIMER_LEFT_20_PENALTY=0.001278
PRIMER_RIGHT_20_PENALTY=2.476717
PRIMER_LEFT_20_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_20_SEQUENCE=CTGTTGTTCTTCCTACCGCTT
PRIMER_LEFT_20=908,20
PRIMER_RIGHT_20=1808,21
PRIMER_LEFT_20_TM=59.999
PRIMER_RIGHT_20_TM=58.523
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=10.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=901
PRIMER_PAIR_21_PENALTY=2.517837
PRIMER_LEFT_21_PENALTY=0.001278
PRIMER_RIGHT_21_PENALTY=2.516559
PRIMER_LEFT_21_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_21_SEQUENCE=TGTTGTTCTTCCTACCGCTT
PRIMER_LEFT_21=908,20
PRIMER_RIGHT_21=1807,20
PRIMER_LEFT_21_TM=59.999
PRIMER_RIGHT_21_TM=57.483
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=10.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=900
PRIMER_PAIR_22_PENALTY=2.542273
PRIMER_LEFT_22_PENALTY=0.385764
PRIMER_RIGHT_22_PENALTY=2.156509
PRIMER_LEFT_22_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_22_SEQUENCE=GCATCGCATATTCAGGAGACA
PRIMER_LEFT_22=907,20
PRIMER_RIGHT_22=1726,21
PRIMER_LEFT_22_TM=59.614
PRIMER_RIGHT_22_TM=61.157
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=820
PRIMER_PAIR_23_PENALTY=2.574862
PRIMER_LEFT_23_PENALTY=0.001278
PRIMER_RIGHT_23_PENALTY=2.573584
PRIMER_LEFT_23_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_23_SEQUENCE=GGTTTGATCCATTCCAAAGA
PRIMER_LEFT_23=908,20
PRIMER_RIGHT_23=1582,20
PRIMER_LEFT_23_TM=59.999
PRIMER_RIGHT_23_TM=57.426
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=675
PRIMER_PAIR_24_PENALTY=2.599811
PRIMER_LEFT_24_PENALTY=0.385764
PRIMER_RIGHT_24_PENALTY=2.214047
PRIMER_LEFT_24_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_24_SEQUENCE=CGCATCGCATATTCAGGAGA
PRIMER_LEFT_24=907,20
PRIMER_RIGHT_24=1727,20
PRIMER_LEFT_24_TM=59.614
PRIMER_RIGHT_24_TM=62.214
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=821
PRIMER_PAIR_25_PENALTY=2.744905
PRIMER_LEFT_25_PENALTY=0.001278
PRIMER_RIGHT_25_PENALTY=2.743627
PRIMER_LEFT_25_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_25_SEQUENCE=TTCTGCTGGGTTTGATCCATT
PRIMER_LEFT_25=908,20
PRIMER_RIGHT_25=1590,21
PRIMER_LEFT_25_TM=59.999
PRIMER_RIGHT_25_TM=61.744
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=683
PRIMER_PAIR_26_PENALTY=2.787359
PRIMER_LEFT_26_PENALTY=0.001278
PRIMER_RIGHT_26_PENALTY=2.786081
PRIMER_LEFT_26_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_26_SEQUENCE=CATCGCATATTCAGGAGACA
PRIMER_LEFT_26=908,20
PRIMER_RIGHT_26=1725,20
PRIMER_LEFT_26_TM=59.999
PRIMER_RIGHT_26_TM=57.214
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=818
PRIMER_PAIR_27_PENALTY=2.808323
PRIMER_LEFT_27_PENALTY=2.633239
PRIMER_RIGHT_27_PENALTY=0.175084
PRIMER_LEFT_27_SEQUENCE=AACGCAGATAAAAGTGGGAA
PRIMER_RIGHT_27_SEQUENCE=TTCTGCTTGTGTTCTGCTGG
PRIMER_LEFT_27=623,20
PRIMER_RIGHT_27=1601,20
PRIMER_LEFT_27_TM=57.367
PRIMER_RIGHT_27_TM=60.175
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=979
PRIMER_PAIR_28_PENALTY=2.808323
PRIMER_LEFT_28_PENALTY=2.633239
PRIMER_RIGHT_28_PENALTY=0.175084
PRIMER_LEFT_28_SEQUENCE=AAACGCAGATAAAAGTGGGA
PRIMER_RIGHT_28_SEQUENCE=TTCTGCTTGTGTTCTGCTGG
PRIMER_LEFT_28=622,20
PRIMER_RIGHT_28=1601,20
PRIMER_LEFT_28_TM=57.367
PRIMER_RIGHT_28_TM=60.175
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=980
PRIMER_PAIR_29_PENALTY=2.832167
PRIMER_LEFT_29_PENALTY=0.385764
PRIMER_RIGHT_29_PENALTY=2.446403
PRIMER_LEFT_29_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_29_SEQUENCE=TGCCATTCCAAAATCAGCAA
PRIMER_LEFT_29=907,20
PRIMER_RIGHT_29=1558,20
PRIMER_LEFT_29_TM=59.614
PRIMER_RIGHT_29_TM=62.446
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=652
PRIMER_PAIR_30_PENALTY=2.899175
PRIMER_LEFT_30_PENALTY=2.460004
PRIMER_RIGHT_30_PENALTY=0.439172
PRIMER_LEFT_30_SEQUENCE=GAGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_30_SEQUENCE=CCTTGCCATTCCAAAATCAG
PRIMER_LEFT_30=906,21
PRIMER_RIGHT_30=1561,20
PRIMER_LEFT_30_TM=61.460
PRIMER_RIGHT_30_TM=60.439
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=656
PRIMER_PAIR_31_PENALTY=2.911223
PRIMER_LEFT_31_PENALTY=2.633239
PRIMER_RIGHT_31_PENALTY=0.277985
PRIMER_LEFT_31_SEQUENCE=AACGCAGATAAAAGTGGGAA
PRIMER_RIGHT_31_SEQUENCE=TTGTGTTCTGCTGGGTTTGA
PRIMER_LEFT_31=623,20
PRIMER_RIGHT_31=1595,20
PRIMER_LEFT_31_TM=57.367
PRIMER_RIGHT_31_TM=60.278
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.7000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=973
PRIMER_PAIR_32_PENALTY=2.911223
PRIMER_LEFT_32_PENALTY=2.633239
PRIMER_RIGHT_32_PENALTY=0.277985
PRIMER_LEFT_32_SEQUENCE=AAACGCAGATAAAAGTGGGA
PRIMER_RIGHT_32_SEQUENCE=TTGTGTTCTGCTGGGTTTGA
PRIMER_LEFT_32=622,20
PRIMER_RIGHT_32=1595,20
PRIMER_LEFT_32_TM=57.367
PRIMER_RIGHT_32_TM=60.278
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=9.7000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=974
PRIMER_PAIR_33_PENALTY=2.920869
PRIMER_LEFT_33_PENALTY=2.460004
PRIMER_RIGHT_33_PENALTY=0.460865
PRIMER_LEFT_33_SEQUENCE=GAGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_33_SEQUENCE=TCTGCTGGGTTTGATCCATT
PRIMER_LEFT_33=906,21
PRIMER_RIGHT_33=1589,20
PRIMER_LEFT_33_TM=61.460
PRIMER_RIGHT_33_TM=60.461
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=8.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=684
PRIMER_PAIR_34_PENALTY=2.932136
PRIMER_LEFT_34_PENALTY=2.633239
PRIMER_RIGHT_34_PENALTY=0.298898
PRIMER_LEFT_34_SEQUENCE=AACGCAGATAAAAGTGGGAA
PRIMER_RIGHT_34_SEQUENCE=GCTTGTGTTCTGCTGGGTTT
PRIMER_LEFT_34=623,20
PRIMER_RIGHT_34=1597,20
PRIMER_LEFT_34_TM=57.367
PRIMER_RIGHT_34_TM=60.299
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=975
PRIMER_PAIR_35_PENALTY=2.955350
PRIMER_LEFT_35_PENALTY=2.460004
PRIMER_RIGHT_35_PENALTY=0.495347
PRIMER_LEFT_35_SEQUENCE=GAGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_35_SEQUENCE=AGATCCTTGCCATTCCAAAA
PRIMER_LEFT_35=906,21
PRIMER_RIGHT_35=1565,20
PRIMER_LEFT_35_TM=61.460
PRIMER_RIGHT_35_TM=59.505
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=660
PRIMER_PAIR_36_PENALTY=2.959348
PRIMER_LEFT_36_PENALTY=0.385764
PRIMER_RIGHT_36_PENALTY=2.573584
PRIMER_LEFT_36_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_36_SEQUENCE=GGTTTGATCCATTCCAAAGA
PRIMER_LEFT_36=907,20
PRIMER_RIGHT_36=1582,20
PRIMER_LEFT_36_TM=59.614
PRIMER_RIGHT_36_TM=57.426
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=676
PRIMER_PAIR_37_PENALTY=2.975749
PRIMER_LEFT_37_PENALTY=0.001278
PRIMER_RIGHT_37_PENALTY=2.974471
PRIMER_LEFT_37_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_37_SEQUENCE=AGGAGACATGTAACCGCTGGA
PRIMER_LEFT_37=908,20
PRIMER_RIGHT_37=1713,21
PRIMER_LEFT_37_TM=59.999
PRIMER_RIGHT_37_TM=61.974
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=806
PRIMER_PAIR_38_PENALTY=3.072410
PRIMER_LEFT_38_PENALTY=2.633239
PRIMER_RIGHT_38_PENALTY=0.439172
PRIMER_LEFT_38_SEQUENCE=AACGCAGATAAAAGTGGGAA
PRIMER_RIGHT_38_SEQUENCE=CCTTGCCATTCCAAAATCAG
PRIMER_LEFT_38=623,20
PRIMER_RIGHT_38=1561,20
PRIMER_LEFT_38_TM=57.367
PRIMER_RIGHT_38_TM=60.439
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=939
PRIMER_PAIR_39_PENALTY=3.072410
PRIMER_LEFT_39_PENALTY=2.633239
PRIMER_RIGHT_39_PENALTY=0.439172
PRIMER_LEFT_39_SEQUENCE=AAACGCAGATAAAAGTGGGA
PRIMER_RIGHT_39_SEQUENCE=CCTTGCCATTCCAAAATCAG
PRIMER_LEFT_39=622,20
PRIMER_RIGHT_39=1561,20
PRIMER_LEFT_39_TM=57.367
PRIMER_RIGHT_39_TM=60.439
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=940
PRIMER_PAIR_40_PENALTY=3.094104
PRIMER_LEFT_40_PENALTY=2.633239
PRIMER_RIGHT_40_PENALTY=0.460865
PRIMER_LEFT_40_SEQUENCE=AACGCAGATAAAAGTGGGAA
PRIMER_RIGHT_40_SEQUENCE=TCTGCTGGGTTTGATCCATT
PRIMER_LEFT_40=623,20
PRIMER_RIGHT_40=1589,20
PRIMER_LEFT_40_TM=57.367
PRIMER_RIGHT_40_TM=60.461
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.7000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=967
PRIMER_PAIR_41_PENALTY=3.094104
PRIMER_LEFT_41_PENALTY=2.633239
PRIMER_RIGHT_41_PENALTY=0.460865
PRIMER_LEFT_41_SEQUENCE=AAACGCAGATAAAAGTGGGA
PRIMER_RIGHT_41_SEQUENCE=TCTGCTGGGTTTGATCCATT
PRIMER_LEFT_41=622,20
PRIMER_RIGHT_41=1589,20
PRIMER_LEFT_41_TM=57.367
PRIMER_RIGHT_41_TM=60.461
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=8.4000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=968
PRIMER_PAIR_42_PENALTY=3.128585
PRIMER_LEFT_42_PENALTY=2.633239
PRIMER_RIGHT_42_PENALTY=0.495347
PRIMER_LEFT_42_SEQUENCE=AACGCAGATAAAAGTGGGAA
PRIMER_RIGHT_42_SEQUENCE=AGATCCTTGCCATTCCAAAA
PRIMER_LEFT_42=623,20
PRIMER_RIGHT_42=1565,20
PRIMER_LEFT_42_TM=57.367
PRIMER_RIGHT_42_TM=59.505
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=943
PRIMER_PAIR_43_PENALTY=3.128585
PRIMER_LEFT_43_PENALTY=2.633239
PRIMER_RIGHT_43_PENALTY=0.495347
PRIMER_LEFT_43_SEQUENCE=AAACGCAGATAAAAGTGGGA
PRIMER_RIGHT_43_SEQUENCE=AGATCCTTGCCATTCCAAAA
PRIMER_LEFT_43=622,20
PRIMER_RIGHT_43=1565,20
PRIMER_LEFT_43_TM=57.367
PRIMER_RIGHT_43_TM=59.505
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.7000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=944
PRIMER_PAIR_44_PENALTY=3.129392
PRIMER_LEFT_44_PENALTY=0.385764
PRIMER_RIGHT_44_PENALTY=2.743627
PRIMER_LEFT_44_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_44_SEQUENCE=TTCTGCTGGGTTTGATCCATT
PRIMER_LEFT_44=907,20
PRIMER_RIGHT_44=1590,21
PRIMER_LEFT_44_TM=59.614
PRIMER_RIGHT_44_TM=61.744
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=684
PRIMER_PAIR_45_PENALTY=3.171845
PRIMER_LEFT_45_PENALTY=0.385764
PRIMER_RIGHT_45_PENALTY=2.786081
PRIMER_LEFT_45_SEQUENCE=AGCAATTCAAGCTGCAACAA
PRIMER_RIGHT_45_SEQUENCE=CATCGCATATTCAGGAGACA
PRIMER_LEFT_45=907,20
PRIMER_RIGHT_45=1725,20
PRIMER_LEFT_45_TM=59.614
PRIMER_RIGHT_45_TM=57.214
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=819
PRIMER_PAIR_46_PENALTY=3.209809
PRIMER_LEFT_46_PENALTY=0.001278
PRIMER_RIGHT_46_PENALTY=3.208531
PRIMER_LEFT_46_SEQUENCE=GCAATTCAAGCTGCAACAAA
PRIMER_RIGHT_46_SEQUENCE=TCCTTGCCATTCCAAAATCAG
PRIMER_LEFT_46=908,20
PRIMER_RIGHT_46=1562,21
PRIMER_LEFT_46_TM=59.999
PRIMER_RIGHT_46_TM=62.209
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.6000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=655
PRIMER_PAIR_47_PENALTY=3.298708
PRIMER_LEFT_47_PENALTY=2.633239
PRIMER_RIGHT_47_PENALTY=0.665470
PRIMER_LEFT_47_SEQUENCE=AACGCAGATAAAAGTGGGAA
PRIMER_RIGHT_47_SEQUENCE=CTTGTGTTCTGCTGGGTTTG
PRIMER_LEFT_47=623,20
PRIMER_RIGHT_47=1596,20
PRIMER_LEFT_47_TM=57.367
PRIMER_RIGHT_47_TM=59.335
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=974
PRIMER_PAIR_48_PENALTY=3.298708
PRIMER_LEFT_48_PENALTY=2.633239
PRIMER_RIGHT_48_PENALTY=0.665470
PRIMER_LEFT_48_SEQUENCE=AAACGCAGATAAAAGTGGGA
PRIMER_RIGHT_48_SEQUENCE=CTTGTGTTCTGCTGGGTTTG
PRIMER_LEFT_48=622,20
PRIMER_RIGHT_48=1596,20
PRIMER_LEFT_48_TM=57.367
PRIMER_RIGHT_48_TM=59.335
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.7000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=975
PRIMER_PAIR_49_PENALTY=3.382513
PRIMER_LEFT_49_PENALTY=3.207428
PRIMER_RIGHT_49_PENALTY=0.175084
PRIMER_LEFT_49_SEQUENCE=AGTGGGAATTCAAATGTGGT
PRIMER_RIGHT_49_SEQUENCE=TTCTGCTTGTGTTCTGCTGG
PRIMER_LEFT_49=635,20
PRIMER_RIGHT_49=1601,20
PRIMER_LEFT_49_TM=56.793
PRIMER_RIGHT_49_TM=60.175
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.6000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=967
=
SEQUENCE_ID=MAGIC.329_4_10319409_10319864_oy-0_ler-0_365191_253491_TRUE
SEQUENCE_TARGET=950,555
SEQUENCE_TEMPLATE=TATNGGGAAAACNCCACCGCCATATTTCTCTTGTAGGTAATNTCCCGCGTTAACCAGTCCTATACTATCAGCTTGTTTGGTNCCATAAAACCCTTTCGCNAAAACCGCNGTNACAAACANATTGATCATGAACGAAACAAAAAGCGCGGCTGANGATTCGATTGTATAGTANTTNAGAGCTTCTTGAACTCTGTTTATCTCCTTTGGATCCGTCTTCCTAGACTGCACTAAAGCTGAATGCAAGAACACATTNTGAGGCGTTATTACACATCCNACAACTCCAACAGCTTCTCTNATCGTCTTTGATCCGAGTTTCGGAATAATAATACCTTTCAAAATCATCATCAAGATAAAACCAAATCAAGAAATGNNATCAAATAAGAGACAATGCTTAATCTTACAAACTTACCTATGAATAGTTCTTCAACACTTGGCTTAGTTTCATTAAACATNCAAGCGAACGAAAGCGCCATTGTCGCAATCAAAACCGCGAAAAGACCTTCTAATTTCCTCATTCCACATTTCTCCAAATACGAAATCAAGAAGCTGNTTTTTCACNAACAACNCAAATCAAAANTNNNNTTNNNAACAACACCGCAAAAAGACCTATTTTGTTTTTTAATAACTCAGGTGATCCTAAAGGCGTTNNNNNNNANAGACCTANTTTTGCTTACCAATCAAANGATGTGATGATAACACCAACCCAAATAGGTAAAAANCCTCGAGTCAAAATCTGAAGAGCGNTCGCGCTTCCGATGACTTCTTGAATATCAGCTCCGATCAAAGCAACNTCCGCCATGAACCAAAGCAAGATTCGCGCCCACGANGGATACTCACTTCGACAAATCTCCGCTAAATGTCGACCCGTNGCGACACCGATACGAGCAGAGAGAAGNTGCATCANAAGTCCCATTAGCGTNGCCCATAGAAGAAGCCANAGGAGAGAGTANCCAGCCACAGCTCCGGCTTGTAAATCTCCTTCGATNTTTCCTGGATCTANNAANGCGATGCTCATGAGAAATCCAGGTCCGGTGAATTTCCATAGCTTNGCCCATGAGAATGGCGGAACTGAATCGTAGGTTTTNTCTCTGTCTTCTTCTACGTTCAGGANCTGATTCTCCGGTGGNGATTCGTGAAGCTCGTCCTCCTCTGGCTGAGAGGTNTCGGGAACTAATAGACGTTTGAATTCGCCGTTACTGTCGTTTGGTCTCTTNGGGCTGTTTTCTTGGCGGGAAACGGTNGANNCCGTCATCTTAGTAACGGAATATTCTTTCGGNGTTACTGACGACGTGANTTGTTTTAAGAAAATTTTAANAGATTACATGGATTATTTTGANNNNNNTAAAATTAAAGTCAAGAATAGTCAAATGAGAAAACAAAAGCAAATTCTAAAAATGAANTGTTATNAAAAGTTTCTATTATTAGAAACTGAATTGTGATCTCTATTTTTTTGACNAAAATTTTCTAAATTGGTATAACAAAAATTAGTTGTGTTNAAGGATTAGCACAATAAATATTGGTTTAGAGATATTTGCGGATTGAATTAANAACAAACCATAGACCAGAACCGGTTAAATTACAAAATCCCTNAACCGGTACAGAATCAGACCANAGCTAAGCAATTTTCAAACAAGCAAATCAAAAGGNATAACATAGNTTGTTTTCCTAAAAAAAAGCAAACATCTTCAAAAACAAAATTTGAATGTAAAATTTNAAAACCGATTTCAGCTTTAGGACATAAGACCATTTAGTTGGAGCAGCAACCAGTTTTCTTNACTGCGGAAACATCAACATCGATCTTCTCTCCTTTNGAAGGAACATTACCGGAATCTTCACCAGCCTCCATTGCTTTCTTGCTCACAACATGGTAAATCTGAGTGAGTACTTCAGAAAACGCGTTCTCAACATTGGTAGATTCAAGAGCTGAGGTTTCCATGAAGTAGAGTGATTCATTCTCTGCAAAAGATTTTGCATCTTCGGTTTGGACTGCTACAANATGGCGAAGATCGGATTTGTTTCCCACAAGCATCACAACTATGTTTGGGTCAGTNTGGTCACGGAGTTCCCTTAGCCATCTCTCAACGTTTTNGAATGTTGAGTGTCGGGTTACGTCGTANACAAGAAGCGCNCCAACAGCTCCTCNNTAGTAAGCACTAGTGATTGCTCGGTATCTAAAAATAGCNAAAAGAGGAGAAACAAAAACCCGTTAACGACTTANTCATTGAAACTCTGTTAAATTAGAAAGATAGGAACATAATAAGAACCGAAATGACAAGAAAGACTTCAAAGCAATAAGCTCTTCANCTTCAATGGTAGAAAACGATCATTAAAGAAATCGATATCGTCATCAGAATTAACGAACATATAGAAACNCCATGAACATATACGATACCGAAGATTATCATCAACCACTGNTTGATCNAACTAATCAGCAAAANAANNGAATTNCTACAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.905995
PRIMER_LEFT_0_PENALTY=0.861164
PRIMER_RIGHT_0_PENALTY=0.044832
PRIMER_LEFT_0_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_0_SEQUENCE=GAGCAAGAAAGCAATGGAGG
PRIMER_LEFT_0=873,20
PRIMER_RIGHT_0=1858,20
PRIMER_LEFT_0_TM=59.139
PRIMER_RIGHT_0_TM=59.955
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=9.4000
PRIMER_PAIR_0_COMPL_ANY=2.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=986
PRIMER_PAIR_1_PENALTY=0.919387
PRIMER_LEFT_1_PENALTY=0.396944
PRIMER_RIGHT_1_PENALTY=0.522443
PRIMER_LEFT_1_SEQUENCE=CACTTCGACAAATCTCCGCT
PRIMER_RIGHT_1_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_1=834,20
PRIMER_RIGHT_1=1772,20
PRIMER_LEFT_1_TM=60.397
PRIMER_RIGHT_1_TM=59.478
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=939
PRIMER_PAIR_2_PENALTY=1.057876
PRIMER_LEFT_2_PENALTY=1.013045
PRIMER_RIGHT_2_PENALTY=0.044832
PRIMER_LEFT_2_SEQUENCE=GACACCGATACGAGCAGAGA
PRIMER_RIGHT_2_SEQUENCE=GAGCAAGAAAGCAATGGAGG
PRIMER_LEFT_2=871,20
PRIMER_RIGHT_2=1858,20
PRIMER_LEFT_2_TM=58.987
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=988
PRIMER_PAIR_3_PENALTY=1.072424
PRIMER_LEFT_3_PENALTY=1.027592
PRIMER_RIGHT_3_PENALTY=0.044832
PRIMER_LEFT_3_SEQUENCE=ACACCGATACGAGCAGAGAGA
PRIMER_RIGHT_3_SEQUENCE=GAGCAAGAAAGCAATGGAGG
PRIMER_LEFT_3=872,21
PRIMER_RIGHT_3=1858,20
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=987
PRIMER_PAIR_4_PENALTY=1.072424
PRIMER_LEFT_4_PENALTY=1.027592
PRIMER_RIGHT_4_PENALTY=0.044832
PRIMER_LEFT_4_SEQUENCE=GACACCGATACGAGCAGAGAG
PRIMER_RIGHT_4_SEQUENCE=GAGCAAGAAAGCAATGGAGG
PRIMER_LEFT_4=871,21
PRIMER_RIGHT_4=1858,20
PRIMER_LEFT_4_TM=60.028
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=988
PRIMER_PAIR_5_PENALTY=1.106492
PRIMER_LEFT_5_PENALTY=0.015253
PRIMER_RIGHT_5_PENALTY=1.091240
PRIMER_LEFT_5_SEQUENCE=AACAACACCGCAAAAAGACC
PRIMER_RIGHT_5_SEQUENCE=CCGGTTCTGGTCTATGGTTT
PRIMER_LEFT_5=587,20
PRIMER_RIGHT_5=1570,20
PRIMER_LEFT_5_TM=60.015
PRIMER_RIGHT_5_TM=58.909
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=984
PRIMER_PAIR_6_PENALTY=1.272165
PRIMER_LEFT_6_PENALTY=0.015253
PRIMER_RIGHT_6_PENALTY=1.256912
PRIMER_LEFT_6_SEQUENCE=AACAACACCGCAAAAAGACC
PRIMER_RIGHT_6_SEQUENCE=ACCGGTTCTGGTCTATGGTTT
PRIMER_LEFT_6=587,20
PRIMER_RIGHT_6=1571,21
PRIMER_LEFT_6_TM=60.015
PRIMER_RIGHT_6_TM=59.743
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=985
PRIMER_PAIR_7_PENALTY=1.383607
PRIMER_LEFT_7_PENALTY=0.861164
PRIMER_RIGHT_7_PENALTY=0.522443
PRIMER_LEFT_7_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_7_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_7=873,20
PRIMER_RIGHT_7=1772,20
PRIMER_LEFT_7_TM=59.139
PRIMER_RIGHT_7_TM=59.478
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=900
PRIMER_PAIR_8_PENALTY=1.488183
PRIMER_LEFT_8_PENALTY=0.396944
PRIMER_RIGHT_8_PENALTY=1.091240
PRIMER_LEFT_8_SEQUENCE=CACTTCGACAAATCTCCGCT
PRIMER_RIGHT_8_SEQUENCE=CCGGTTCTGGTCTATGGTTT
PRIMER_LEFT_8=834,20
PRIMER_RIGHT_8=1570,20
PRIMER_LEFT_8_TM=60.397
PRIMER_RIGHT_8_TM=58.909
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=11.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=737
PRIMER_PAIR_9_PENALTY=1.550035
PRIMER_LEFT_9_PENALTY=1.027592
PRIMER_RIGHT_9_PENALTY=0.522443
PRIMER_LEFT_9_SEQUENCE=ACACCGATACGAGCAGAGAGA
PRIMER_RIGHT_9_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_9=872,21
PRIMER_RIGHT_9=1772,20
PRIMER_LEFT_9_TM=60.028
PRIMER_RIGHT_9_TM=59.478
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=901
PRIMER_PAIR_10_PENALTY=1.550035
PRIMER_LEFT_10_PENALTY=1.027592
PRIMER_RIGHT_10_PENALTY=0.522443
PRIMER_LEFT_10_SEQUENCE=GACACCGATACGAGCAGAGAG
PRIMER_RIGHT_10_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_10=871,21
PRIMER_RIGHT_10=1772,20
PRIMER_LEFT_10_TM=60.028
PRIMER_RIGHT_10_TM=59.478
PRIMER_LEFT_10_GC_PERCENT=57.143
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=902
PRIMER_PAIR_11_PENALTY=1.588939
PRIMER_LEFT_11_PENALTY=1.544107
PRIMER_RIGHT_11_PENALTY=0.044832
PRIMER_LEFT_11_SEQUENCE=CACCGATACGAGCAGAGAGAA
PRIMER_RIGHT_11_SEQUENCE=GAGCAAGAAAGCAATGGAGG
PRIMER_LEFT_11=873,21
PRIMER_RIGHT_11=1858,20
PRIMER_LEFT_11_TM=60.544
PRIMER_RIGHT_11_TM=59.955
PRIMER_LEFT_11_GC_PERCENT=52.381
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=986
PRIMER_PAIR_12_PENALTY=1.638152
PRIMER_LEFT_12_PENALTY=0.015253
PRIMER_RIGHT_12_PENALTY=1.622900
PRIMER_LEFT_12_SEQUENCE=AACAACACCGCAAAAAGACC
PRIMER_RIGHT_12_SEQUENCE=ACCGGTTCTGGTCTATGGTT
PRIMER_LEFT_12=587,20
PRIMER_RIGHT_12=1571,20
PRIMER_LEFT_12_TM=60.015
PRIMER_RIGHT_12_TM=58.377
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=985
PRIMER_PAIR_13_PENALTY=1.653856
PRIMER_LEFT_13_PENALTY=0.396944
PRIMER_RIGHT_13_PENALTY=1.256912
PRIMER_LEFT_13_SEQUENCE=CACTTCGACAAATCTCCGCT
PRIMER_RIGHT_13_SEQUENCE=ACCGGTTCTGGTCTATGGTTT
PRIMER_LEFT_13=834,20
PRIMER_RIGHT_13=1571,21
PRIMER_LEFT_13_TM=60.397
PRIMER_RIGHT_13_TM=59.743
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=738
PRIMER_PAIR_14_PENALTY=1.660549
PRIMER_LEFT_14_PENALTY=0.396944
PRIMER_RIGHT_14_PENALTY=1.263605
PRIMER_LEFT_14_SEQUENCE=CACTTCGACAAATCTCCGCT
PRIMER_RIGHT_14_SEQUENCE=TGCTGCTCCAACTAAATGGTC
PRIMER_LEFT_14=834,20
PRIMER_RIGHT_14=1761,21
PRIMER_LEFT_14_TM=60.397
PRIMER_RIGHT_14_TM=60.264
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=11.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=928
PRIMER_PAIR_15_PENALTY=1.712751
PRIMER_LEFT_15_PENALTY=0.861164
PRIMER_RIGHT_15_PENALTY=0.851588
PRIMER_LEFT_15_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_15_SEQUENCE=TGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_15=873,20
PRIMER_RIGHT_15=1859,20
PRIMER_LEFT_15_TM=59.139
PRIMER_RIGHT_15_TM=59.148
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=987
PRIMER_PAIR_16_PENALTY=1.738509
PRIMER_LEFT_16_PENALTY=1.216066
PRIMER_RIGHT_16_PENALTY=0.522443
PRIMER_LEFT_16_SEQUENCE=TCGACAAATCTCCGCTAAATG
PRIMER_RIGHT_16_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_16=838,21
PRIMER_RIGHT_16=1772,20
PRIMER_LEFT_16_TM=60.216
PRIMER_RIGHT_16_TM=59.478
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=935
PRIMER_PAIR_17_PENALTY=1.764783
PRIMER_LEFT_17_PENALTY=0.861164
PRIMER_RIGHT_17_PENALTY=0.903619
PRIMER_LEFT_17_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_17_SEQUENCE=CAAGAAAGCAATGGAGGCTG
PRIMER_LEFT_17=873,20
PRIMER_RIGHT_17=1855,20
PRIMER_LEFT_17_TM=59.139
PRIMER_RIGHT_17_TM=60.904
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=983
PRIMER_PAIR_18_PENALTY=1.825262
PRIMER_LEFT_18_PENALTY=0.396944
PRIMER_RIGHT_18_PENALTY=1.428318
PRIMER_LEFT_18_SEQUENCE=CACTTCGACAAATCTCCGCT
PRIMER_RIGHT_18_SEQUENCE=GATGTTGATGTTTCCGCAGT
PRIMER_LEFT_18=834,20
PRIMER_RIGHT_18=1794,20
PRIMER_LEFT_18_TM=60.397
PRIMER_RIGHT_18_TM=58.572
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=11.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=961
PRIMER_PAIR_19_PENALTY=1.859328
PRIMER_LEFT_19_PENALTY=0.861164
PRIMER_RIGHT_19_PENALTY=0.998165
PRIMER_LEFT_19_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_19_SEQUENCE=GTGAGCAAGAAAGCAATGGA
PRIMER_LEFT_19=873,20
PRIMER_RIGHT_19=1860,20
PRIMER_LEFT_19_TM=59.139
PRIMER_RIGHT_19_TM=59.002
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=988
PRIMER_PAIR_20_PENALTY=1.864633
PRIMER_LEFT_20_PENALTY=1.013045
PRIMER_RIGHT_20_PENALTY=0.851588
PRIMER_LEFT_20_SEQUENCE=GACACCGATACGAGCAGAGA
PRIMER_RIGHT_20_SEQUENCE=TGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_20=871,20
PRIMER_RIGHT_20=1859,20
PRIMER_LEFT_20_TM=58.987
PRIMER_RIGHT_20_TM=59.148
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=989
PRIMER_PAIR_21_PENALTY=1.868833
PRIMER_LEFT_21_PENALTY=0.861164
PRIMER_RIGHT_21_PENALTY=1.007669
PRIMER_LEFT_21_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_21_SEQUENCE=GTGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_21=873,20
PRIMER_RIGHT_21=1860,21
PRIMER_LEFT_21_TM=59.139
PRIMER_RIGHT_21_TM=60.008
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=988
PRIMER_PAIR_22_PENALTY=1.879180
PRIMER_LEFT_22_PENALTY=1.027592
PRIMER_RIGHT_22_PENALTY=0.851588
PRIMER_LEFT_22_SEQUENCE=ACACCGATACGAGCAGAGAGA
PRIMER_RIGHT_22_SEQUENCE=TGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_22=872,21
PRIMER_RIGHT_22=1859,20
PRIMER_LEFT_22_TM=60.028
PRIMER_RIGHT_22_TM=59.148
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=988
PRIMER_PAIR_23_PENALTY=1.879180
PRIMER_LEFT_23_PENALTY=1.027592
PRIMER_RIGHT_23_PENALTY=0.851588
PRIMER_LEFT_23_SEQUENCE=GACACCGATACGAGCAGAGAG
PRIMER_RIGHT_23_SEQUENCE=TGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_23=871,21
PRIMER_RIGHT_23=1859,20
PRIMER_LEFT_23_TM=60.028
PRIMER_RIGHT_23_TM=59.148
PRIMER_LEFT_23_GC_PERCENT=57.143
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=989
PRIMER_PAIR_24_PENALTY=1.903540
PRIMER_LEFT_24_PENALTY=0.861164
PRIMER_RIGHT_24_PENALTY=1.042377
PRIMER_LEFT_24_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_24_SEQUENCE=CATGTTGTGAGCAAGAAAGCA
PRIMER_LEFT_24=873,20
PRIMER_RIGHT_24=1866,21
PRIMER_LEFT_24_TM=59.139
PRIMER_RIGHT_24_TM=60.042
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=994
PRIMER_PAIR_25_PENALTY=1.916664
PRIMER_LEFT_25_PENALTY=1.013045
PRIMER_RIGHT_25_PENALTY=0.903619
PRIMER_LEFT_25_SEQUENCE=GACACCGATACGAGCAGAGA
PRIMER_RIGHT_25_SEQUENCE=CAAGAAAGCAATGGAGGCTG
PRIMER_LEFT_25=871,20
PRIMER_RIGHT_25=1855,20
PRIMER_LEFT_25_TM=58.987
PRIMER_RIGHT_25_TM=60.904
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=985
PRIMER_PAIR_26_PENALTY=1.931212
PRIMER_LEFT_26_PENALTY=1.027592
PRIMER_RIGHT_26_PENALTY=0.903619
PRIMER_LEFT_26_SEQUENCE=ACACCGATACGAGCAGAGAGA
PRIMER_RIGHT_26_SEQUENCE=CAAGAAAGCAATGGAGGCTG
PRIMER_LEFT_26=872,21
PRIMER_RIGHT_26=1855,20
PRIMER_LEFT_26_TM=60.028
PRIMER_RIGHT_26_TM=60.904
PRIMER_LEFT_26_GC_PERCENT=52.381
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=984
PRIMER_PAIR_27_PENALTY=1.931212
PRIMER_LEFT_27_PENALTY=1.027592
PRIMER_RIGHT_27_PENALTY=0.903619
PRIMER_LEFT_27_SEQUENCE=GACACCGATACGAGCAGAGAG
PRIMER_RIGHT_27_SEQUENCE=CAAGAAAGCAATGGAGGCTG
PRIMER_LEFT_27=871,21
PRIMER_RIGHT_27=1855,20
PRIMER_LEFT_27_TM=60.028
PRIMER_RIGHT_27_TM=60.904
PRIMER_LEFT_27_GC_PERCENT=57.143
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=985
PRIMER_PAIR_28_PENALTY=1.938535
PRIMER_LEFT_28_PENALTY=0.015253
PRIMER_RIGHT_28_PENALTY=1.923282
PRIMER_LEFT_28_SEQUENCE=AACAACACCGCAAAAAGACC
PRIMER_RIGHT_28_SEQUENCE=CGGTTCTGGTCTATGGTTTG
PRIMER_LEFT_28=587,20
PRIMER_RIGHT_28=1569,20
PRIMER_LEFT_28_TM=60.015
PRIMER_RIGHT_28_TM=58.077
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=983
PRIMER_PAIR_29_PENALTY=1.952403
PRIMER_LEFT_29_PENALTY=0.861164
PRIMER_RIGHT_29_PENALTY=1.091240
PRIMER_LEFT_29_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_29_SEQUENCE=CCGGTTCTGGTCTATGGTTT
PRIMER_LEFT_29=873,20
PRIMER_RIGHT_29=1570,20
PRIMER_LEFT_29_TM=59.139
PRIMER_RIGHT_29_TM=58.909
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=7.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=698
PRIMER_PAIR_30_PENALTY=1.986684
PRIMER_LEFT_30_PENALTY=1.941852
PRIMER_RIGHT_30_PENALTY=0.044832
PRIMER_LEFT_30_SEQUENCE=ACACCGATACGAGCAGAGAG
PRIMER_RIGHT_30_SEQUENCE=GAGCAAGAAAGCAATGGAGG
PRIMER_LEFT_30=872,20
PRIMER_RIGHT_30=1858,20
PRIMER_LEFT_30_TM=58.058
PRIMER_RIGHT_30_TM=59.955
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=2.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=987
PRIMER_PAIR_31_PENALTY=1.998166
PRIMER_LEFT_31_PENALTY=1.953334
PRIMER_RIGHT_31_PENALTY=0.044832
PRIMER_LEFT_31_SEQUENCE=CGACACCGATACGAGCAGAG
PRIMER_RIGHT_31_SEQUENCE=GAGCAAGAAAGCAATGGAGG
PRIMER_LEFT_31=870,20
PRIMER_RIGHT_31=1858,20
PRIMER_LEFT_31_TM=61.953
PRIMER_RIGHT_31_TM=59.955
PRIMER_LEFT_31_GC_PERCENT=60.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=989
PRIMER_PAIR_32_PENALTY=2.011210
PRIMER_LEFT_32_PENALTY=1.013045
PRIMER_RIGHT_32_PENALTY=0.998165
PRIMER_LEFT_32_SEQUENCE=GACACCGATACGAGCAGAGA
PRIMER_RIGHT_32_SEQUENCE=GTGAGCAAGAAAGCAATGGA
PRIMER_LEFT_32=871,20
PRIMER_RIGHT_32=1860,20
PRIMER_LEFT_32_TM=58.987
PRIMER_RIGHT_32_TM=59.002
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=990
PRIMER_PAIR_33_PENALTY=2.014512
PRIMER_LEFT_33_PENALTY=0.861164
PRIMER_RIGHT_33_PENALTY=1.153349
PRIMER_LEFT_33_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_33_SEQUENCE=AGAAAGCAATGGAGGCTGGT
PRIMER_LEFT_33=873,20
PRIMER_RIGHT_33=1853,20
PRIMER_LEFT_33_TM=59.139
PRIMER_RIGHT_33_TM=61.153
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=981
PRIMER_PAIR_34_PENALTY=2.016077
PRIMER_LEFT_34_PENALTY=1.493634
PRIMER_RIGHT_34_PENALTY=0.522443
PRIMER_LEFT_34_SEQUENCE=TTCGACAAATCTCCGCTAAA
PRIMER_RIGHT_34_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_34=837,20
PRIMER_RIGHT_34=1772,20
PRIMER_LEFT_34_TM=58.506
PRIMER_RIGHT_34_TM=59.478
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.3000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=936
PRIMER_PAIR_35_PENALTY=2.019843
PRIMER_LEFT_35_PENALTY=0.396944
PRIMER_RIGHT_35_PENALTY=1.622900
PRIMER_LEFT_35_SEQUENCE=CACTTCGACAAATCTCCGCT
PRIMER_RIGHT_35_SEQUENCE=ACCGGTTCTGGTCTATGGTT
PRIMER_LEFT_35=834,20
PRIMER_RIGHT_35=1571,20
PRIMER_LEFT_35_TM=60.397
PRIMER_RIGHT_35_TM=58.377
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=11.4000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=738
PRIMER_PAIR_36_PENALTY=2.020714
PRIMER_LEFT_36_PENALTY=1.013045
PRIMER_RIGHT_36_PENALTY=1.007669
PRIMER_LEFT_36_SEQUENCE=GACACCGATACGAGCAGAGA
PRIMER_RIGHT_36_SEQUENCE=GTGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_36=871,20
PRIMER_RIGHT_36=1860,21
PRIMER_LEFT_36_TM=58.987
PRIMER_RIGHT_36_TM=60.008
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=990
PRIMER_PAIR_37_PENALTY=2.025757
PRIMER_LEFT_37_PENALTY=1.027592
PRIMER_RIGHT_37_PENALTY=0.998165
PRIMER_LEFT_37_SEQUENCE=ACACCGATACGAGCAGAGAGA
PRIMER_RIGHT_37_SEQUENCE=GTGAGCAAGAAAGCAATGGA
PRIMER_LEFT_37=872,21
PRIMER_RIGHT_37=1860,20
PRIMER_LEFT_37_TM=60.028
PRIMER_RIGHT_37_TM=59.002
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.1000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=989
PRIMER_PAIR_38_PENALTY=2.025757
PRIMER_LEFT_38_PENALTY=1.027592
PRIMER_RIGHT_38_PENALTY=0.998165
PRIMER_LEFT_38_SEQUENCE=GACACCGATACGAGCAGAGAG
PRIMER_RIGHT_38_SEQUENCE=GTGAGCAAGAAAGCAATGGA
PRIMER_LEFT_38=871,21
PRIMER_RIGHT_38=1860,20
PRIMER_LEFT_38_TM=60.028
PRIMER_RIGHT_38_TM=59.002
PRIMER_LEFT_38_GC_PERCENT=57.143
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=8.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=990
PRIMER_PAIR_39_PENALTY=2.035261
PRIMER_LEFT_39_PENALTY=1.027592
PRIMER_RIGHT_39_PENALTY=1.007669
PRIMER_LEFT_39_SEQUENCE=ACACCGATACGAGCAGAGAGA
PRIMER_RIGHT_39_SEQUENCE=GTGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_39=872,21
PRIMER_RIGHT_39=1860,21
PRIMER_LEFT_39_TM=60.028
PRIMER_RIGHT_39_TM=60.008
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=989
PRIMER_PAIR_40_PENALTY=2.035261
PRIMER_LEFT_40_PENALTY=1.027592
PRIMER_RIGHT_40_PENALTY=1.007669
PRIMER_LEFT_40_SEQUENCE=GACACCGATACGAGCAGAGAG
PRIMER_RIGHT_40_SEQUENCE=GTGAGCAAGAAAGCAATGGAG
PRIMER_LEFT_40=871,21
PRIMER_RIGHT_40=1860,21
PRIMER_LEFT_40_TM=60.028
PRIMER_RIGHT_40_TM=60.008
PRIMER_LEFT_40_GC_PERCENT=57.143
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=990
PRIMER_PAIR_41_PENALTY=2.039672
PRIMER_LEFT_41_PENALTY=1.517229
PRIMER_RIGHT_41_PENALTY=0.522443
PRIMER_LEFT_41_SEQUENCE=CTTCGACAAATCTCCGCTAAA
PRIMER_RIGHT_41_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_41=836,21
PRIMER_RIGHT_41=1772,20
PRIMER_LEFT_41_TM=59.483
PRIMER_RIGHT_41_TM=59.478
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.3000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=937
PRIMER_PAIR_42_PENALTY=2.055422
PRIMER_LEFT_42_PENALTY=1.013045
PRIMER_RIGHT_42_PENALTY=1.042377
PRIMER_LEFT_42_SEQUENCE=GACACCGATACGAGCAGAGA
PRIMER_RIGHT_42_SEQUENCE=CATGTTGTGAGCAAGAAAGCA
PRIMER_LEFT_42=871,20
PRIMER_RIGHT_42=1866,21
PRIMER_LEFT_42_TM=58.987
PRIMER_RIGHT_42_TM=60.042
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=996
PRIMER_PAIR_43_PENALTY=2.066550
PRIMER_LEFT_43_PENALTY=1.544107
PRIMER_RIGHT_43_PENALTY=0.522443
PRIMER_LEFT_43_SEQUENCE=CACCGATACGAGCAGAGAGAA
PRIMER_RIGHT_43_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_43=873,21
PRIMER_RIGHT_43=1772,20
PRIMER_LEFT_43_TM=60.544
PRIMER_RIGHT_43_TM=59.478
PRIMER_LEFT_43_GC_PERCENT=52.381
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=9.4000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=900
PRIMER_PAIR_44_PENALTY=2.069969
PRIMER_LEFT_44_PENALTY=1.027592
PRIMER_RIGHT_44_PENALTY=1.042377
PRIMER_LEFT_44_SEQUENCE=ACACCGATACGAGCAGAGAGA
PRIMER_RIGHT_44_SEQUENCE=CATGTTGTGAGCAAGAAAGCA
PRIMER_LEFT_44=872,21
PRIMER_RIGHT_44=1866,21
PRIMER_LEFT_44_TM=60.028
PRIMER_RIGHT_44_TM=60.042
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=995
PRIMER_PAIR_45_PENALTY=2.069969
PRIMER_LEFT_45_PENALTY=1.027592
PRIMER_RIGHT_45_PENALTY=1.042377
PRIMER_LEFT_45_SEQUENCE=GACACCGATACGAGCAGAGAG
PRIMER_RIGHT_45_SEQUENCE=CATGTTGTGAGCAAGAAAGCA
PRIMER_LEFT_45=871,21
PRIMER_RIGHT_45=1866,21
PRIMER_LEFT_45_TM=60.028
PRIMER_RIGHT_45_TM=60.042
PRIMER_LEFT_45_GC_PERCENT=57.143
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=996
PRIMER_PAIR_46_PENALTY=2.118076
PRIMER_LEFT_46_PENALTY=0.861164
PRIMER_RIGHT_46_PENALTY=1.256912
PRIMER_LEFT_46_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_46_SEQUENCE=ACCGGTTCTGGTCTATGGTTT
PRIMER_LEFT_46=873,20
PRIMER_RIGHT_46=1571,21
PRIMER_LEFT_46_TM=59.139
PRIMER_RIGHT_46_TM=59.743
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=699
PRIMER_PAIR_47_PENALTY=2.124769
PRIMER_LEFT_47_PENALTY=0.861164
PRIMER_RIGHT_47_PENALTY=1.263605
PRIMER_LEFT_47_SEQUENCE=CACCGATACGAGCAGAGAGA
PRIMER_RIGHT_47_SEQUENCE=TGCTGCTCCAACTAAATGGTC
PRIMER_LEFT_47=873,20
PRIMER_RIGHT_47=1761,21
PRIMER_LEFT_47_TM=59.139
PRIMER_RIGHT_47_TM=60.264
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=889
PRIMER_PAIR_48_PENALTY=2.135424
PRIMER_LEFT_48_PENALTY=1.612981
PRIMER_RIGHT_48_PENALTY=0.522443
PRIMER_LEFT_48_SEQUENCE=CGACAAATCTCCGCTAAATG
PRIMER_RIGHT_48_SEQUENCE=AGAAAACTGGTTGCTGCTCC
PRIMER_LEFT_48=839,20
PRIMER_RIGHT_48=1772,20
PRIMER_LEFT_48_TM=58.387
PRIMER_RIGHT_48_TM=59.478
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.2000
PRIMER_RIGHT_48_END_STABILITY=9.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=934
PRIMER_PAIR_49_PENALTY=2.166394
PRIMER_LEFT_49_PENALTY=1.013045
PRIMER_RIGHT_49_PENALTY=1.153349
PRIMER_LEFT_49_SEQUENCE=GACACCGATACGAGCAGAGA
PRIMER_RIGHT_49_SEQUENCE=AGAAAGCAATGGAGGCTGGT
PRIMER_LEFT_49=871,20
PRIMER_RIGHT_49=1853,20
PRIMER_LEFT_49_TM=58.987
PRIMER_RIGHT_49_TM=61.153
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=983
=
SEQUENCE_ID=MAGIC.433_4_4634827_4634908_can-0_bur-0_19430_8812_TRUE
SEQUENCE_TARGET=950,181
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANGNTTGAAAANTCTGTTGAGGCNNTNGTGGATTCNCCATGAAGTCGGGCTTCGCGAAATCCTNGACCACCAAATGGTCATTACCANCTCTTGCAAACAATGCATCCGAGCTTTTGNGNNGGNGCTCCACGGANGAGGCNNATNNNNNNNNNNNNTTGAAAANGAGANGGTAACCAACTCTAGAAGACATGGTCNNNNNGCCATACNTTTCNAAACAAACNNANGNTTNCNAATTATNCAANCAANNGATTANNACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGNTAACACCTNCTCCGTAGNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTAACNGATCTCGACAAANGTACCTNTNNANCCNNCNTNCNATATGAAGCTTAGATTTTAATGTTAGTAACACGGATGATCGCTAGATGTATCATTGCTTANAAAATTNTCAAAAAAATATANANTTTATAGTAAATTATGCAAACATGGCAAAATCTTTTATCCATAGATTTGAATGTGTTGCAATAATGTTAGTGACTAACTTTGTAATAGTCACAAGAGTTGATACATCAAGAATAAATCAGATAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_15652114_15652772_rsch-4_zu-0_130684_38943_TRUE
SEQUENCE_TARGET=950,758
SEQUENCE_TEMPLATE=TAAGGANATGACNGGGATCATAGGTTTTCTGAAGTTCCATTNATATTATCTCAAGAANAAAAANGTTTTACTTCGTCTAAAGATAAAATCAAAACCAANNNAGAGTNTAGAACAAAAGTGANTAGATAAACTACAGCTCCTGAGAAATGATAATGGCGATTCCTTCCNATACTTCNTGAAATCTATCCTCCTGCATTAGGAAATCGAATCAGGATCCATGTTCACTAAAGTATATATATCATGGACAATCTATTATACTTNAAGGNNTAATCTAANTGCTCTTAANTACAATGCACAACCTTTCAAATAGATATAGCCTTGTTGACATGGTTTCTCCATTAGAGAAGCAAAATGAAACAACTAATCTTNAATNTCAAGNTACAGAATCAGTGNTGTTCAATTCTACTGAAATGAATGCAATAAANGGGACTATTGGATCAAAANTAAAACTGAAATGAGTTTGTTGTTACCTCATATGTTGCTAGGGTACATGAAGACTCTGACTCTAGCTCCCTGAGATTAAACACAGNAGATNAAAAATGATTAGTCTGGAGGAAANAATCCANTTAACCATGATTATGTTTCNNNAAGATAAAGTACCATTGACTTGGGTGGTAGGTTTGANGTGAACTTAGAACGGACAACACCGCTGTTACCATGAGGCCTAGTGACTTTGCCCCAAATGCAACGGTAGTGAGAACCGTTCTTCTTTGTCTTTGCCTTGTAGATGTAAGCCAAACGCTTACCCTTGTACCAANTGACCTCCTCTTGAGTGTTCACACCTTCAATCTGGATGAGAGAAGTGTTGGGATATTGGTTCGACTTGGACCTGAAACATTACTTAATCGTTAGTAATCAATCTTTANTCTGCTTTCTNTAAGCAATCAAACTATCAACCAACTGCTTAAAAGCTAATCTTAAAGGAAGCANNAGCAAAAATGCATCATTTTGCACAACCATTAATCAGAAACAATGGAGTAACATTACAAATNTGAGAGCANTATCATTGAAATCTATCAGTTGTGGCAAAGTTACAAACTTTCATCGAGACCTTATGTTGATATCCAAAAACGATAGGTGAATATTCAAATCCAAATAACTTAAGAACATACAGAGAAAGACTGAAAATTGAAAGTACCTCTTGTAGCCGAGGACTGTTCCACGAACATACAATCTGCATAACAGCAACACAAAGATTTCATCAGATTAAAGTGAACCAGTAACGAACAAATTCAAATCGGAGATTCAGCTGAGAAAAAGAGAGCACAATCAAAANCCTAATTACCTAACTCTCTCTCCTTGACGTCCCTTCATTTTCAANCCTTGTAACTCTCGTCTTCCTCTTCTTACTCTCTTTTANGNCGNCGAGCGAATTCACAGAGAAAAACCCTAGNTNTCGNTATTAGGTCAAGACTTTATATGGATTCATAAATAAGCTCAGCNACAAAACTCTATTGGGCTTATATNNNNNTATCTCAAACAAGTTGGCCCAAACNTNAATACGGCTTGGCCCNNATANGGCNTNGCATAAGAAAATNATTATTTATTTTCTTAATCAATATNGCTTTGCTTAAGAATATGACNNCNNNTNACTTTGTTTCTTCTATTTGTCTCTTGAATTGTTTTGTTAAAAGTAAAAATTTGTGTTGAACAGAAAANTTTTTATATATAATGTTATAAANAATTTATGTCATTTGAAATAATAAAAGTAACAACAAAAGGATTACAATTTTTTTCTTCTTGGTATAATTTTTACATCGTTAACAATGTCGAAGACATTTTAATCAAACATNAGCAAAAACATGGAAGTTCTTGGGAGTCCACAGAGATGAGTCTAACAGCAGCAATAGCAGCAGCAAGAGACATTAATCCAAAGAAGCAAACATTAAGCCAATGCCAANGCTTTTGCACAAGACTAAGCTTATCGTTCATAGCAACAAGATACATGTGATTCGCTAATATGAATGTGAGAGGGAACGTGCTTATTGCTCCAGTGAGGCTCATGAAATCTCCGAGAAACGGTAAAAGAGCCGAGAGAAGAGTGCTCACCGCGATGTAGCTTCCTCTTGCTACNGTTCTAAACAACAGATTTTTTATTNCTAATGGTCTTCATTTGACTCCATACTTTGTGTCCATATACTCATAAGTCGGACTTGCAAAAATTTANGTAAGAAAAACACAAGAATTTAAACGGTTAAGGTTAGGATTAAGACACAAAGACTCATCATTGAGGTCTAGATAGAGACTTACATGTAAAGAGATAACAGATTGGAGAAAAGCTGAAATATTAGCGAGTGCTTTGACCCAAAGAGGTCCACTGACGCTATTAAACAGATAAGTCGATGTCGAGGACCCGTAAGCCCAATATCCGATGAATGTAACCGCATACATCNGTAAAACACCAACAGTGAACTGAAAATANAGAGCTTTCATCATGTTTTTAACGACCGGTTGCTTCACTGTCGCCTAATCATTTGCACAAAAGATTAGTGTAAGTCACAAAANAGAGAAATATTTNATTGACCATAAATAGATCGATGAGACTTGCCTGTATTTCCGGTAGCATTCCCGTGTTGAATGCAAACTGCTCCTGTTATGGTNAAGAGTTTGTTTATTGATGATCCTTGTATGTTGTAATCTCTTTCAGGCTTGTTTACT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_3156302_3156409_kn-0_oy-0_62532_2313351_TRUE
SEQUENCE_TARGET=950,207
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_26374562_26374563_ler-0_rsch-4_177981_974_TRUE
SEQUENCE_TARGET=950,101
SEQUENCE_TEMPLATE=TCTNAAAAAAAAAAAAANAAGATNAAAAAAAAACTCAAGTAGATATATGACATGATATAACATAAAGTAGCAAATGAAATTAGACATTTGAAGGTATCGTAGCATCACTAAATCTATGTTCTCTTTCTGTCATGTCATATATCTATTTGAGTTAATATATAAACGAANTCTAGGAAAAATAACGAATATGTAAACAGAGAGCTGCCTTTTGTGTAAGTTTTAATTNGGTTCAATCTATTTAGGTACGTCCAAGATACAACGAAATGGCTATTTTTATGCATAACAATTCTTTTAGCTAATACCTANNNNNNNNNNNNNNNNNNNNGTAATTGGTAAAGACCATCTATGTCTACGTTTTNTTTAGATTGAGTAGAATCAACGATTCAAAAGTTTTATTGTTAATATATAACGAACAAAAAAAATTGTCGANTTTTTTCTTCACAGAGCACTGACACCAGTTAACTTNTTGTAGGGGTCGACAAATCCCAATTCAAGAGGCAAATCAAACTGTTAAAAACGAGACCAGNGAATGTTTACGAGTAAAATTCGACCGTGGTGTAACGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_6465041_6465469_ws-0_oy-0_1269995_331604_TRUE
SEQUENCE_TARGET=950,528
SEQUENCE_TEMPLATE=AAACTGAAANAAAAATNAAACACACAAGAGACAAAACACGAACCTTCTTCTCACCAAGGAAGTTACGAATCTCAATACTCTTATTGTTACCATCAATAGAAGCATTGATAGGAAAATGAGCATACACAAATCTCATTCTATAAAGAAAACCTTGAGTAACACCAGCAATGAGATTATCAACATGGCTTAAAGCAGTTCTAATCGAAGCACTTGTCTTACGAGAACCAAACCAAGAATCAATCTTAAGCTGACGTTTTCCAGTGACTTGGTCTTTAATCAACTGGAAATCGAGATTCAGATGCTTGAAGTCACGAGTGAGTTTACCTCGTGGACCTTCGACCTCAATCACCTTGGCGTTTACCTTAATGGCNACGCCGTCGGGGATGTCCATNGTCTCCGAGGACAAAATGGTCTTCATTTTTTCGTCTCTGAGGGGGAACTTTGGAGACGACGGCTTGAGGTTTAGGGTANCGGAGTTTAAGGGTACANAGAGANGAAAACCCTAANGNTTATATATAGGTCGTCTCTAGNTTTTGTTTTTGGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNGAGAACANNTNAATGGANGACTGCTTACAACCCNCTTAATTCCATTNAAAACTCANTGGTAGTTAGTAGTTNACTAANGATAANCCATTGTTCTCGTGACGCTNNANAAANAATCGACGGATNNTTNAATTTTTCATCGNTAATCCATAAAGGATTNNANATGTGNTATGATTCGGTAATAACCNTTTTTGTANCATTAGNTTTTNTTCAANTGTANNAAAANNNNNNNNNNNNNNNNNNNNNANCGTGTACAANTACTTTTCCTCGTCATAAGGCCAAACCCTGCTNCNAACTAAAACAACTTAACAATGGTAAGGAAATAAAACACAAGCCATCACGAGATTTGAAAACAAAAAAGNTGCGTACNTTGCTTACATAACNTTATCATCTCAATTTCTANATATTCCTTAATTTCCGTAATTTAAGATNTNTTTTTNTNTTTNANTTGTTTGTTAGGTTNGTCTCTTGTTTNNNCANCATGTCACAATGTGTCTGAGCTTCTNCTATCTNCAAAGCTTTCNTCTATCTCTTTGACTCTCGCTATATATNCCNNCNNNNNTNTATATNNTNAATCCAANNNNNNNGATNNNNNT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_2_4382995_4383088_ler-0_po-0_868653_54288_TRUE
SEQUENCE_TARGET=950,193
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_10478500_10478505_sf-2_oy-0_852850_2601_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAGAAANGAGAAAAAAATNTGTATNAAAANNTNAAAATTAATACATANTTATTGCGGANNATATANACACTAATAATCACTCGTTTACNTGATAAAGGTNNAAAAAAATGAATTGCAACAAGATATTCCATATTNAAAAATATAAGAATGCTAATTGAGATACTTGTTTTTTCCAGCAAAATTAATTTATATTGAATACAACAAAGTAGATAGCCAAGGTGGTGGCATAATACAACCAGAGAATTGAGATGCTTTTAACANAAAAAAGAAATACCAAAAATTGATNNTAAAANAGATAAATAGNATTATAATCGGGGAANANTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_5_12606175_12606665_ws-0_ct-1_68901_86171_TRUE
SEQUENCE_TARGET=950,590
SEQUENCE_TEMPLATE=AGCTTGTNCATCGANTTATTGAAGTGACTCATCTAAACAGTGAGNGTAACAAACTCAGAGAAACCATAAGAAACATTCAACANTTTTGTAACTTTCAAAATGGAATCTATTTGGAATTCCCTGTCAACATCATATATATTGCTAATAATTATGCANATCTGAGAATGTATTAAGTGCATAAATATTTAAAACGAAATTATCATTTTTAATATTGCAGAAACGAAANTATCATTATCATAGTATACTAGNNNNNNNNNNNNTTCAACTACTAATTCAACTAGGGGCATACAAAATAATTAACTAGAATATANAACTATATCTATATAATTATTAATCAAAACTATTAGATGAAAAAAAAGAACTCAGTAAAAGTCATAATTTACACATATACATTTTTAGAGTAGTTAAGGAAAAAGTTAAAACTACTTTTGGGGGCGAGATTAAAAGACATGATGTGGTAGACCCAGAGACACGGAGAAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNGNGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_5_15446550_15446623_ct-1_zu-0_215235_159409_TRUE
SEQUENCE_TARGET=950,173
SEQUENCE_TEMPLATE=ACAGTAGAGGGAACATCAATGAGAACCACGTTGTCGAGTTTCAAGGTGANNNNCNNGGAAATATGTTTTCATCCCAACATTCATAAGAAATTTCNAGCACACGNACATGGCGAGAAACTGCAATTTCAATCCACCGTTNGATATATTCAGGTTCAATATGTGTGTTATACTGATAAAGATAAAAATTGAGTCGCAAGCTTTCTAAGACCGGAGCTCTGTGTAGTGGCAGCTTCTTGTCAATAAAATCCACAAGTCCAACCTCCTCCTCCTTATCATATTGACAAGTGTTGAGAAAAGCCAGAAGATCAGTCTTTGGAGAACCGAAATCGAGTCTANGCAACCACATCCAAAGAAACTCCCAACGCTTAGACAAAATGGAAGTGGATACAGCAACTTTTGTCGGAACAAACGATAAAATCTTCACCAGCACATCATCTGGCAATCCATTGAATATGTCCATATCTTACANGTTTTTTNTTTTTTTNGTCAAACTCTTACAGATTCTAAGTNACTGTNNNNNNNNNNNNNAAAACACCATCAAATGATTTTTTTTCAGGCCAAGCAAACNNNNNNNNNNNNNGAGAAAACACAGAACACAAGCATGACAAAACCCTANAGAGAGCTGTAATGTTGAAATNTGAAACAGGACAAAACCCTGGAAAAAATTAAACCTGTGAATTCCAGTTGGAAGCTCGATTCTGCTAGGAATCCTGAGAAANCCTAAAGAACTGCGAANGCGTTTGGTTACAATATANAAGAAAGAAATGNGGAAGATATCAGAATAAGAATCGTCTTCGTCTCCATAAAAGCGGAGCTTCGAAAATGACAAAACTTCGTCGGTACTCACAGATCATTGAACCGTTCGTCTTCTTTGCATTTAATTGGGTTAACGAATGGTTTTCAACCTTTAATGGGCCTAGGCGTGNGCCAGGTTTNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_16626443_16626995_hi-0_ct-1_5355_57299_TRUE
SEQUENCE_TARGET=950,652
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCANTTTTTTTNNTATCANATCGTATTCNNNNNTTGTATTNTTCNTTTNTNTTATATATTNGAANNTNNTTNANATNAAAAAGNGTTTANCNTNNNNNNNTTGAGNTTATTTANNGAAGATAAGTCTGATCNGATCNTTCTCCNTNGTTANCACAATGTGTATTCANTTACGNTNACTATNTACAACATGTTAGTTGNATAAAATTTAGTTTTGGTATAGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNTATATTCATTTTNANNNNNTTNTAAAANTTANATGNTAAAAACTAGTNGNCATANNATGNGNTANAATGTGTNANANNGANATGAATTTTNAANNATATNTNNGTAAAATTNNANTTTTATGTGNAANATGTTTTTNNCCNANTAGAAATNNNNTAAGTGNANNATTNTTTGTTATGAAGGTTGGAAGTTTGAGAGATTTAGNCGCCATAATGNNATACAATGTNTCAGATGGAGATGAATTTTAAANNATATCTATNTAAAANTNNACTTTTATNTGNGAGATNTTTTTNNCNATTAGAAATNNGGTAAGTNCANNATTNTTTGTTATGAANGTTGNAAGTTNGAGANATTTAGTCNNNATAATGNNATACANTGTNTCANANNGNGATNNATTTTAAANGATATNTNTGTAAAATTCNACTTTTATGTGNGNNNNNNNNNNNNNNNNNNNNNNNTNGGNTAANTGNANNNTTNTTTNNTANNAANNTTGGAAGTTNNAGANATTTNCANTTNGGTTTANTGNAAANTTTCTTAGATANATTAAATATANTNGNNGGNCTCCAGTGTNGATCCTCGTANCTNNTTATGGNTNNCAAACATATCAANTTAATGNTNCATGATATTCCTAATAGTNTCACTAAAACATGAACAATTTTATNTAGNNNCGTACTCNAAANACTNGTATATATCACAATCACATGCATGANNAAACNAGAGTTTGATANATCTTTGCTTATTATCATTGTNGTTTTANTTTTGCTAANTNTACNTTATTNNNTNGACTTTGNNTTTTTNTCTTAATNTATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGACAAAATTAGTTAGNAGATGCATGAATTCNGANGANNCTCGAACAAANNNTTTTTNGTGTACNATCTACACCCACATAGATATNCAAAGTCGNGANNATACATAAATNTATCCATNANCACCCNNATGATCNTCNNGGCTATNTTTGCATNCANCNACGAGTTNGTNNTCCATTATGTCAATATATNCATNATNATAAANTGNTNTCCTAAGNANCATNATTTANTAATTCGGNANNANCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4759124_4759180_edi-0_tsu-0_29388_29445_TRUE
SEQUENCE_TARGET=950,156
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_14478822_14478829_sf-2_can-0_23058_43862_TRUE
SEQUENCE_TARGET=950,107
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_16625460_16625928_tsu-0_can-0_88453_19877_TRUE
SEQUENCE_TARGET=950,568
SEQUENCE_TEMPLATE=TCCCAGCTTTGTACCGGTCAATCCTAAANCTACAAGAAACATCTGTAAGTTAGAAACTACTTCATGATCGTTTAGAAAAAAAGATTGATCTCTGCAAGTCATTTTTTGTCCCCAAGCGAATCTAGCAATTGGAGCATAATCTTACTCAAATAAAGACAGTGGTCTAAAAAAACAAAAGAACAGTGGATCAAACATAATCTCGATTTTTTTAGAATCCAGAATCTATAAGTCAAACATAAGCCTTTTTCTTTAACATACTAGAGTAAGAAACTAGGAATCCAAAAAACAGAATCTTCATAGAGAGTATCAGTTCNAAATTGAATTGAAAATTAAACTTGTTTGAGTACTTGAAATCATCCCCAGCAATTGGATATTAATCAAAGAAGTTGATACGCACCTCTGAGATATTCGAGAAGTGTCATATGAGCCAATCCATCAGGCAAGACTCTACGAACGCCGTTAACGTACAGTAACGCCTCCGTAAACTCATCTCCGATCTCGCCGTCCTTTTTCAGTGAACCCATTTCTCAAATTGAGAGAAAACTCGATTGGGATCGAAACAAAAACGAAGATGGCGAAGTTGANTACGCCAAGTCAAGAGAAGCAAAAAAGGAANAACNTTTTCCTTTTTTAATAATAAAATTCGTGTTCCTTTCNGATAGCATCCACCGAATGAATACGCATCTTCATCATCATCACTTGTGCAGTCAANNNNTTTTTTTNTTTTNTTTTTTTTNTAAAGAACCCAAAATAGAAAGAACGAAATTTAAGTTTGAACAAAGTAAAACAATTGAAGCCTATATGATGTNTTTACAATGCCACTTATATATTTGTTTAACTGAATTTTGCTGATTATGTTTCGTTTATAAATTGTAGTCTGATTAGTTATGACTAGTGTGTTANNNNNTTGTCTTAAGATTTACTTTNAAAGAAATTTTGATGATATTGTTTGTTTTATTTTTGGTTTTGATATATGATGATGATAAGCGTCTTGCATTGCNCAGCATAACTGCTTCACATTCTTTGCTCACTTGAAGTTAGAGGTTGTTCAGNAAAAAAAAATCTGATATTTTATCTCGATGGCGCGCGCATTTAGTCAACAAAGTTGCTGCTTTGAAGAAACATGTATCATGTTACATCATGTATTTGGAGACTGGAAGTAACTTACATATATGACTTTGACAAAATGGATTGATCCATCTTTCTCCCCTCTATTAAAGTTTAAAGCTCAAACACTAAGCTTTGGGCAGAAATCTGAATTTGCAAAAGGAGCTGAAAACTCGTCGAAACAAGCCATCCTGATACGCTCTGGAGTGGCTGGAGTCTCCAGAGGGAACCAGTTGGTGAGACCTACCTCGCTTCTAGCCGCTTTGATTGCTTCCTTTATTGCAAAGAAGGCTGATGCTGCAAGAAAGAACGGTGGCTCACCCACTGCTTTAGATGAATGTATTGCCTTTGCATTTGGATTNCCCTGAGNATAATCTCATGTTCAGTTTCAAATGTGTTGCTACCAATTTAAACAGCTTGTTCCTATAGTTGCATACCTTGAGAAGAGAAACATTCAGTTGGAACGGCATGTCGTTGATGGAAGGTATTTTGTAGCTTCCGGGTCCGCAAGTGAGTAAACTTCCTGGTTTAATCCATTTATGAGCTGCATCTCCCCACTTGAGTTCTTCTAGAGCTACCCAACCTAGTCCTTGTACAAACGCTCCTTCTATCTGCAAATGGTGAATTTCAGAAATAACACTTTTCTCTATGATTTTCTTGGATTTTAAACATTGCAAAAGGCCAACGAACAGCTAAAAGCGTAGGATGTTGAGAGAAGATTGCGCACNTGTCCAATATCAATGGTTGGGTTAAGAGAATATCCGAGATCCAACATTATGTCTGCTTTTCTTGTGTGAAAATCACCAGTCAATGTATCNATCTCAACTTCAGCAAAGGCNGCTCCATATGTGTAATATCTATATGCGTTCCCTTTTCCAGATACCCAATCAAATTCAAGTTCTGGAACAATGTGAAAACCGTGAGCTGATAGGTCTATACGTTGAAAGTAGCAGGCACTTNCTAGCTACATACACTCAGAGAAAAAGAGAGTTTTGTTCATCAAAATCTTCTGAGTTTNTATCAAATTGCTAGAAGAAAGAAAGAATACCTCAGAGAATGTGTTGAAATTGTGTTTAGATGCAACAGGCTCCATTCTTGCTATAATCTGCTCACAAGCGTCTAAAACTGCAGCACCATACATATCAGAACTCGCAGAAGCAGCAGTAGGTGACGCATTAGGAACCTGTTACNAGGCAATCAAAACGACAAGAGAGCAAAAACTGTGAGGCATTTGGAACCTATTAAGAGACCACAGAAATGATAAGAGAGAACAACAACAGTTTTGTCTGACTACGTCTTGGCCAAAAGTCCACTGCTTGTAACNGAGTTAAATGTACCTTGTNGGTGCTTGTTTCTGACAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.138306
PRIMER_LEFT_0_PENALTY=1.115965
PRIMER_RIGHT_0_PENALTY=0.022341
PRIMER_LEFT_0_SEQUENCE=ACGCCAAGTCAAGAGAAGCA
PRIMER_RIGHT_0_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_0=586,20
PRIMER_RIGHT_0=1574,20
PRIMER_LEFT_0_TM=61.116
PRIMER_RIGHT_0_TM=59.978
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=989
PRIMER_PAIR_1_PENALTY=1.399117
PRIMER_LEFT_1_PENALTY=1.376776
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=GCCAAGTCAAGAGAAGCAAAA
PRIMER_RIGHT_1_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_1=588,21
PRIMER_RIGHT_1=1574,20
PRIMER_LEFT_1_TM=59.623
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=987
PRIMER_PAIR_2_PENALTY=1.499763
PRIMER_LEFT_2_PENALTY=1.115965
PRIMER_RIGHT_2_PENALTY=0.383798
PRIMER_LEFT_2_SEQUENCE=ACGCCAAGTCAAGAGAAGCA
PRIMER_RIGHT_2_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_2=586,20
PRIMER_RIGHT_2=1576,20
PRIMER_LEFT_2_TM=61.116
PRIMER_RIGHT_2_TM=60.384
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=991
PRIMER_PAIR_3_PENALTY=1.500881
PRIMER_LEFT_3_PENALTY=1.478540
PRIMER_RIGHT_3_PENALTY=0.022341
PRIMER_LEFT_3_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_3_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_3=657,20
PRIMER_RIGHT_3=1574,20
PRIMER_LEFT_3_TM=58.521
PRIMER_RIGHT_3_TM=59.978
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=918
PRIMER_PAIR_4_PENALTY=1.639440
PRIMER_LEFT_4_PENALTY=1.617099
PRIMER_RIGHT_4_PENALTY=0.022341
PRIMER_LEFT_4_SEQUENCE=CGCCAAGTCAAGAGAAGCAA
PRIMER_RIGHT_4_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_4=587,20
PRIMER_RIGHT_4=1574,20
PRIMER_LEFT_4_TM=61.617
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=988
PRIMER_PAIR_5_PENALTY=1.760574
PRIMER_LEFT_5_PENALTY=1.376776
PRIMER_RIGHT_5_PENALTY=0.383798
PRIMER_LEFT_5_SEQUENCE=GCCAAGTCAAGAGAAGCAAAA
PRIMER_RIGHT_5_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_5=588,21
PRIMER_RIGHT_5=1576,20
PRIMER_LEFT_5_TM=59.623
PRIMER_RIGHT_5_TM=60.384
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=989
PRIMER_PAIR_6_PENALTY=1.793938
PRIMER_LEFT_6_PENALTY=1.771597
PRIMER_RIGHT_6_PENALTY=0.022341
PRIMER_LEFT_6_SEQUENCE=GCCAAGTCAAGAGAAGCAAA
PRIMER_RIGHT_6_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_6=588,20
PRIMER_RIGHT_6=1574,20
PRIMER_LEFT_6_TM=58.228
PRIMER_RIGHT_6_TM=59.978
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.8000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=987
PRIMER_PAIR_7_PENALTY=1.862338
PRIMER_LEFT_7_PENALTY=1.478540
PRIMER_RIGHT_7_PENALTY=0.383798
PRIMER_LEFT_7_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_7_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_7=657,20
PRIMER_RIGHT_7=1576,20
PRIMER_LEFT_7_TM=58.521
PRIMER_RIGHT_7_TM=60.384
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=920
PRIMER_PAIR_8_PENALTY=2.000898
PRIMER_LEFT_8_PENALTY=1.617099
PRIMER_RIGHT_8_PENALTY=0.383798
PRIMER_LEFT_8_SEQUENCE=CGCCAAGTCAAGAGAAGCAA
PRIMER_RIGHT_8_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_8=587,20
PRIMER_RIGHT_8=1576,20
PRIMER_LEFT_8_TM=61.617
PRIMER_RIGHT_8_TM=60.384
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=990
PRIMER_PAIR_9_PENALTY=2.106341
PRIMER_LEFT_9_PENALTY=2.084000
PRIMER_RIGHT_9_PENALTY=0.022341
PRIMER_LEFT_9_SEQUENCE=TACGCCAAGTCAAGAGAAGCA
PRIMER_RIGHT_9_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_9=585,21
PRIMER_RIGHT_9=1574,20
PRIMER_LEFT_9_TM=61.084
PRIMER_RIGHT_9_TM=59.978
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=990
PRIMER_PAIR_10_PENALTY=2.125527
PRIMER_LEFT_10_PENALTY=2.103187
PRIMER_RIGHT_10_PENALTY=0.022341
PRIMER_LEFT_10_SEQUENCE=CGCATCTTCATCATCATCACTT
PRIMER_RIGHT_10_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_10=679,22
PRIMER_RIGHT_10=1574,20
PRIMER_LEFT_10_TM=60.103
PRIMER_RIGHT_10_TM=59.978
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=896
PRIMER_PAIR_11_PENALTY=2.155395
PRIMER_LEFT_11_PENALTY=1.771597
PRIMER_RIGHT_11_PENALTY=0.383798
PRIMER_LEFT_11_SEQUENCE=GCCAAGTCAAGAGAAGCAAA
PRIMER_RIGHT_11_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_11=588,20
PRIMER_RIGHT_11=1576,20
PRIMER_LEFT_11_TM=58.228
PRIMER_RIGHT_11_TM=60.384
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.8000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=989
PRIMER_PAIR_12_PENALTY=2.332284
PRIMER_LEFT_12_PENALTY=2.309943
PRIMER_RIGHT_12_PENALTY=0.022341
PRIMER_LEFT_12_SEQUENCE=TCATCATCACTTGTGCAGTCAA
PRIMER_RIGHT_12_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_12=689,22
PRIMER_RIGHT_12=1574,20
PRIMER_LEFT_12_TM=60.310
PRIMER_RIGHT_12_TM=59.978
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=886
PRIMER_PAIR_13_PENALTY=2.335785
PRIMER_LEFT_13_PENALTY=2.313444
PRIMER_RIGHT_13_PENALTY=0.022341
PRIMER_LEFT_13_SEQUENCE=CGCATCTTCATCATCATCACT
PRIMER_RIGHT_13_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_13=679,21
PRIMER_RIGHT_13=1574,20
PRIMER_LEFT_13_TM=58.687
PRIMER_RIGHT_13_TM=59.978
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=896
PRIMER_PAIR_14_PENALTY=2.437497
PRIMER_LEFT_14_PENALTY=2.415156
PRIMER_RIGHT_14_PENALTY=0.022341
PRIMER_LEFT_14_SEQUENCE=ACGCATCTTCATCATCATCACT
PRIMER_RIGHT_14_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_14=678,22
PRIMER_RIGHT_14=1574,20
PRIMER_LEFT_14_TM=59.585
PRIMER_RIGHT_14_TM=59.978
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=897
PRIMER_PAIR_15_PENALTY=2.467798
PRIMER_LEFT_15_PENALTY=2.084000
PRIMER_RIGHT_15_PENALTY=0.383798
PRIMER_LEFT_15_SEQUENCE=TACGCCAAGTCAAGAGAAGCA
PRIMER_RIGHT_15_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_15=585,21
PRIMER_RIGHT_15=1576,20
PRIMER_LEFT_15_TM=61.084
PRIMER_RIGHT_15_TM=60.384
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=992
PRIMER_PAIR_16_PENALTY=2.486985
PRIMER_LEFT_16_PENALTY=2.103187
PRIMER_RIGHT_16_PENALTY=0.383798
PRIMER_LEFT_16_SEQUENCE=CGCATCTTCATCATCATCACTT
PRIMER_RIGHT_16_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_16=679,22
PRIMER_RIGHT_16=1576,20
PRIMER_LEFT_16_TM=60.103
PRIMER_RIGHT_16_TM=60.384
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=898
PRIMER_PAIR_17_PENALTY=2.693741
PRIMER_LEFT_17_PENALTY=2.309943
PRIMER_RIGHT_17_PENALTY=0.383798
PRIMER_LEFT_17_SEQUENCE=TCATCATCACTTGTGCAGTCAA
PRIMER_RIGHT_17_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_17=689,22
PRIMER_RIGHT_17=1576,20
PRIMER_LEFT_17_TM=60.310
PRIMER_RIGHT_17_TM=60.384
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=888
PRIMER_PAIR_18_PENALTY=2.697243
PRIMER_LEFT_18_PENALTY=2.313444
PRIMER_RIGHT_18_PENALTY=0.383798
PRIMER_LEFT_18_SEQUENCE=CGCATCTTCATCATCATCACT
PRIMER_RIGHT_18_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_18=679,21
PRIMER_RIGHT_18=1576,20
PRIMER_LEFT_18_TM=58.687
PRIMER_RIGHT_18_TM=60.384
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=898
PRIMER_PAIR_19_PENALTY=2.761192
PRIMER_LEFT_19_PENALTY=2.738851
PRIMER_RIGHT_19_PENALTY=0.022341
PRIMER_LEFT_19_SEQUENCE=CATCATCACTTGTGCAGTCAA
PRIMER_RIGHT_19_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_19=690,21
PRIMER_RIGHT_19=1574,20
PRIMER_LEFT_19_TM=58.261
PRIMER_RIGHT_19_TM=59.978
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=885
PRIMER_PAIR_20_PENALTY=2.798955
PRIMER_LEFT_20_PENALTY=2.415156
PRIMER_RIGHT_20_PENALTY=0.383798
PRIMER_LEFT_20_SEQUENCE=ACGCATCTTCATCATCATCACT
PRIMER_RIGHT_20_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_20=678,22
PRIMER_RIGHT_20=1576,20
PRIMER_LEFT_20_TM=59.585
PRIMER_RIGHT_20_TM=60.384
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=899
PRIMER_PAIR_21_PENALTY=2.853218
PRIMER_LEFT_21_PENALTY=2.830877
PRIMER_RIGHT_21_PENALTY=0.022341
PRIMER_LEFT_21_SEQUENCE=CATCATCATCACTTGTGCAGTC
PRIMER_RIGHT_21_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_21=687,22
PRIMER_RIGHT_21=1574,20
PRIMER_LEFT_21_TM=59.169
PRIMER_RIGHT_21_TM=59.978
PRIMER_LEFT_21_GC_PERCENT=45.455
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=888
PRIMER_PAIR_22_PENALTY=2.898062
PRIMER_LEFT_22_PENALTY=1.478540
PRIMER_RIGHT_22_PENALTY=1.419522
PRIMER_LEFT_22_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_22_SEQUENCE=CCCGGAAGCTACAAAATACC
PRIMER_LEFT_22=657,20
PRIMER_RIGHT_22=1609,20
PRIMER_LEFT_22_TM=58.521
PRIMER_RIGHT_22_TM=58.580
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.9000
PRIMER_RIGHT_22_END_STABILITY=6.8000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=953
PRIMER_PAIR_23_PENALTY=2.899334
PRIMER_LEFT_23_PENALTY=2.876993
PRIMER_RIGHT_23_PENALTY=0.022341
PRIMER_LEFT_23_SEQUENCE=GCCAAGTCAAGAGAAGCAAAAA
PRIMER_RIGHT_23_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_23=588,22
PRIMER_RIGHT_23=1574,20
PRIMER_LEFT_23_TM=60.877
PRIMER_RIGHT_23_TM=59.978
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=987
PRIMER_PAIR_24_PENALTY=2.968526
PRIMER_LEFT_24_PENALTY=1.478540
PRIMER_RIGHT_24_PENALTY=1.489986
PRIMER_LEFT_24_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_24_SEQUENCE=CCCGGAAGCTACAAAATACCT
PRIMER_LEFT_24=657,20
PRIMER_RIGHT_24=1609,21
PRIMER_LEFT_24_TM=58.521
PRIMER_RIGHT_24_TM=59.510
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.9000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=953
PRIMER_PAIR_25_PENALTY=3.093355
PRIMER_LEFT_25_PENALTY=1.478540
PRIMER_RIGHT_25_PENALTY=1.614815
PRIMER_LEFT_25_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_25_SEQUENCE=ACCCGGAAGCTACAAAATACC
PRIMER_LEFT_25=657,20
PRIMER_RIGHT_25=1610,21
PRIMER_LEFT_25_TM=58.521
PRIMER_RIGHT_25_TM=59.385
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.9000
PRIMER_RIGHT_25_END_STABILITY=6.8000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=954
PRIMER_PAIR_26_PENALTY=3.122650
PRIMER_LEFT_26_PENALTY=2.738851
PRIMER_RIGHT_26_PENALTY=0.383798
PRIMER_LEFT_26_SEQUENCE=CATCATCACTTGTGCAGTCAA
PRIMER_RIGHT_26_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_26=690,21
PRIMER_RIGHT_26=1576,20
PRIMER_LEFT_26_TM=58.261
PRIMER_RIGHT_26_TM=60.384
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=887
PRIMER_PAIR_27_PENALTY=3.214675
PRIMER_LEFT_27_PENALTY=2.830877
PRIMER_RIGHT_27_PENALTY=0.383798
PRIMER_LEFT_27_SEQUENCE=CATCATCATCACTTGTGCAGTC
PRIMER_RIGHT_27_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_27=687,22
PRIMER_RIGHT_27=1576,20
PRIMER_LEFT_27_TM=59.169
PRIMER_RIGHT_27_TM=60.384
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=890
PRIMER_PAIR_28_PENALTY=3.260792
PRIMER_LEFT_28_PENALTY=2.876993
PRIMER_RIGHT_28_PENALTY=0.383798
PRIMER_LEFT_28_SEQUENCE=GCCAAGTCAAGAGAAGCAAAAA
PRIMER_RIGHT_28_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_28=588,22
PRIMER_RIGHT_28=1576,20
PRIMER_LEFT_28_TM=60.877
PRIMER_RIGHT_28_TM=60.384
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=6.6000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=989
PRIMER_PAIR_29_PENALTY=3.289682
PRIMER_LEFT_29_PENALTY=1.376776
PRIMER_RIGHT_29_PENALTY=1.912906
PRIMER_LEFT_29_SEQUENCE=GCCAAGTCAAGAGAAGCAAAA
PRIMER_RIGHT_29_SEQUENCE=CCGTTCCAACTGAATGTTTCT
PRIMER_LEFT_29=588,21
PRIMER_RIGHT_29=1573,21
PRIMER_LEFT_29_TM=59.623
PRIMER_RIGHT_29_TM=59.087
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=986
PRIMER_PAIR_30_PENALTY=3.387387
PRIMER_LEFT_30_PENALTY=3.365046
PRIMER_RIGHT_30_PENALTY=0.022341
PRIMER_LEFT_30_SEQUENCE=ACGCCAAGTCAAGAGAAGCAA
PRIMER_RIGHT_30_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_30=586,21
PRIMER_RIGHT_30=1574,20
PRIMER_LEFT_30_TM=62.365
PRIMER_RIGHT_30_TM=59.978
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=989
PRIMER_PAIR_31_PENALTY=3.391446
PRIMER_LEFT_31_PENALTY=1.478540
PRIMER_RIGHT_31_PENALTY=1.912906
PRIMER_LEFT_31_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_31_SEQUENCE=CCGTTCCAACTGAATGTTTCT
PRIMER_LEFT_31=657,20
PRIMER_RIGHT_31=1573,21
PRIMER_LEFT_31_TM=58.521
PRIMER_RIGHT_31_TM=59.087
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=917
PRIMER_PAIR_32_PENALTY=3.522708
PRIMER_LEFT_32_PENALTY=2.103187
PRIMER_RIGHT_32_PENALTY=1.419522
PRIMER_LEFT_32_SEQUENCE=CGCATCTTCATCATCATCACTT
PRIMER_RIGHT_32_SEQUENCE=CCCGGAAGCTACAAAATACC
PRIMER_LEFT_32=679,22
PRIMER_RIGHT_32=1609,20
PRIMER_LEFT_32_TM=60.103
PRIMER_RIGHT_32_TM=58.580
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.8000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=931
PRIMER_PAIR_33_PENALTY=3.593172
PRIMER_LEFT_33_PENALTY=2.103187
PRIMER_RIGHT_33_PENALTY=1.489986
PRIMER_LEFT_33_SEQUENCE=CGCATCTTCATCATCATCACTT
PRIMER_RIGHT_33_SEQUENCE=CCCGGAAGCTACAAAATACCT
PRIMER_LEFT_33=679,22
PRIMER_RIGHT_33=1609,21
PRIMER_LEFT_33_TM=60.103
PRIMER_RIGHT_33_TM=59.510
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=931
PRIMER_PAIR_34_PENALTY=3.650297
PRIMER_LEFT_34_PENALTY=1.478540
PRIMER_RIGHT_34_PENALTY=2.171757
PRIMER_LEFT_34_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_34_SEQUENCE=GGAAGTTTACTCACTTGCGGAC
PRIMER_LEFT_34=657,20
PRIMER_RIGHT_34=1630,22
PRIMER_LEFT_34_TM=58.521
PRIMER_RIGHT_34_TM=60.172
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=9.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=974
PRIMER_PAIR_35_PENALTY=3.684503
PRIMER_LEFT_35_PENALTY=1.771597
PRIMER_RIGHT_35_PENALTY=1.912906
PRIMER_LEFT_35_SEQUENCE=GCCAAGTCAAGAGAAGCAAA
PRIMER_RIGHT_35_SEQUENCE=CCGTTCCAACTGAATGTTTCT
PRIMER_LEFT_35=588,20
PRIMER_RIGHT_35=1573,21
PRIMER_LEFT_35_TM=58.228
PRIMER_RIGHT_35_TM=59.087
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.8000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=986
PRIMER_PAIR_36_PENALTY=3.718002
PRIMER_LEFT_36_PENALTY=2.103187
PRIMER_RIGHT_36_PENALTY=1.614815
PRIMER_LEFT_36_SEQUENCE=CGCATCTTCATCATCATCACTT
PRIMER_RIGHT_36_SEQUENCE=ACCCGGAAGCTACAAAATACC
PRIMER_LEFT_36=679,22
PRIMER_RIGHT_36=1610,21
PRIMER_LEFT_36_TM=60.103
PRIMER_RIGHT_36_TM=59.385
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.8000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=932
PRIMER_PAIR_37_PENALTY=3.727918
PRIMER_LEFT_37_PENALTY=1.478540
PRIMER_RIGHT_37_PENALTY=2.249378
PRIMER_LEFT_37_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_37_SEQUENCE=ACCCGGAAGCTACAAAATACCT
PRIMER_LEFT_37=657,20
PRIMER_RIGHT_37=1610,22
PRIMER_LEFT_37_TM=58.521
PRIMER_RIGHT_37_TM=60.249
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=954
PRIMER_PAIR_38_PENALTY=3.729464
PRIMER_LEFT_38_PENALTY=2.309943
PRIMER_RIGHT_38_PENALTY=1.419522
PRIMER_LEFT_38_SEQUENCE=TCATCATCACTTGTGCAGTCAA
PRIMER_RIGHT_38_SEQUENCE=CCCGGAAGCTACAAAATACC
PRIMER_LEFT_38=689,22
PRIMER_RIGHT_38=1609,20
PRIMER_LEFT_38_TM=60.310
PRIMER_RIGHT_38_TM=58.580
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.8000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=921
PRIMER_PAIR_39_PENALTY=3.732966
PRIMER_LEFT_39_PENALTY=2.313444
PRIMER_RIGHT_39_PENALTY=1.419522
PRIMER_LEFT_39_SEQUENCE=CGCATCTTCATCATCATCACT
PRIMER_RIGHT_39_SEQUENCE=CCCGGAAGCTACAAAATACC
PRIMER_LEFT_39=679,21
PRIMER_RIGHT_39=1609,20
PRIMER_LEFT_39_TM=58.687
PRIMER_RIGHT_39_TM=58.580
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=6.8000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=931
PRIMER_PAIR_40_PENALTY=3.748844
PRIMER_LEFT_40_PENALTY=3.365046
PRIMER_RIGHT_40_PENALTY=0.383798
PRIMER_LEFT_40_SEQUENCE=ACGCCAAGTCAAGAGAAGCAA
PRIMER_RIGHT_40_SEQUENCE=ATGCCGTTCCAACTGAATGT
PRIMER_LEFT_40=586,21
PRIMER_RIGHT_40=1576,20
PRIMER_LEFT_40_TM=62.365
PRIMER_RIGHT_40_TM=60.384
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=991
PRIMER_PAIR_41_PENALTY=3.799928
PRIMER_LEFT_41_PENALTY=2.309943
PRIMER_RIGHT_41_PENALTY=1.489986
PRIMER_LEFT_41_SEQUENCE=TCATCATCACTTGTGCAGTCAA
PRIMER_RIGHT_41_SEQUENCE=CCCGGAAGCTACAAAATACCT
PRIMER_LEFT_41=689,22
PRIMER_RIGHT_41=1609,21
PRIMER_LEFT_41_TM=60.310
PRIMER_RIGHT_41_TM=59.510
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=921
PRIMER_PAIR_42_PENALTY=3.803430
PRIMER_LEFT_42_PENALTY=2.313444
PRIMER_RIGHT_42_PENALTY=1.489986
PRIMER_LEFT_42_SEQUENCE=CGCATCTTCATCATCATCACT
PRIMER_RIGHT_42_SEQUENCE=CCCGGAAGCTACAAAATACCT
PRIMER_LEFT_42=679,21
PRIMER_RIGHT_42=1609,21
PRIMER_LEFT_42_TM=58.687
PRIMER_RIGHT_42_TM=59.510
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=931
PRIMER_PAIR_43_PENALTY=3.834678
PRIMER_LEFT_43_PENALTY=2.415156
PRIMER_RIGHT_43_PENALTY=1.419522
PRIMER_LEFT_43_SEQUENCE=ACGCATCTTCATCATCATCACT
PRIMER_RIGHT_43_SEQUENCE=CCCGGAAGCTACAAAATACC
PRIMER_LEFT_43=678,22
PRIMER_RIGHT_43=1609,20
PRIMER_LEFT_43_TM=59.585
PRIMER_RIGHT_43_TM=58.580
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=6.8000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=932
PRIMER_PAIR_44_PENALTY=3.843641
PRIMER_LEFT_44_PENALTY=3.821300
PRIMER_RIGHT_44_PENALTY=0.022341
PRIMER_LEFT_44_SEQUENCE=CGCCAAGTCAAGAGAAGCAAA
PRIMER_RIGHT_44_SEQUENCE=GCCGTTCCAACTGAATGTTT
PRIMER_LEFT_44=587,21
PRIMER_RIGHT_44=1574,20
PRIMER_LEFT_44_TM=62.821
PRIMER_RIGHT_44_TM=59.978
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.8000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=988
PRIMER_PAIR_45_PENALTY=3.905142
PRIMER_LEFT_45_PENALTY=2.415156
PRIMER_RIGHT_45_PENALTY=1.489986
PRIMER_LEFT_45_SEQUENCE=ACGCATCTTCATCATCATCACT
PRIMER_RIGHT_45_SEQUENCE=CCCGGAAGCTACAAAATACCT
PRIMER_LEFT_45=678,22
PRIMER_RIGHT_45=1609,21
PRIMER_LEFT_45_TM=59.585
PRIMER_RIGHT_45_TM=59.510
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=932
PRIMER_PAIR_46_PENALTY=3.924758
PRIMER_LEFT_46_PENALTY=2.309943
PRIMER_RIGHT_46_PENALTY=1.614815
PRIMER_LEFT_46_SEQUENCE=TCATCATCACTTGTGCAGTCAA
PRIMER_RIGHT_46_SEQUENCE=ACCCGGAAGCTACAAAATACC
PRIMER_LEFT_46=689,22
PRIMER_RIGHT_46=1610,21
PRIMER_LEFT_46_TM=60.310
PRIMER_RIGHT_46_TM=59.385
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=6.8000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=922
PRIMER_PAIR_47_PENALTY=3.928259
PRIMER_LEFT_47_PENALTY=2.313444
PRIMER_RIGHT_47_PENALTY=1.614815
PRIMER_LEFT_47_SEQUENCE=CGCATCTTCATCATCATCACT
PRIMER_RIGHT_47_SEQUENCE=ACCCGGAAGCTACAAAATACC
PRIMER_LEFT_47=679,21
PRIMER_RIGHT_47=1610,21
PRIMER_LEFT_47_TM=58.687
PRIMER_RIGHT_47_TM=59.385
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=6.8000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=932
PRIMER_PAIR_48_PENALTY=3.955257
PRIMER_LEFT_48_PENALTY=1.478540
PRIMER_RIGHT_48_PENALTY=2.476717
PRIMER_LEFT_48_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_48_SEQUENCE=AGGAAGTTTACTCACTTGCGG
PRIMER_LEFT_48=657,20
PRIMER_RIGHT_48=1631,21
PRIMER_LEFT_48_TM=58.521
PRIMER_RIGHT_48_TM=58.523
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=11.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=975
PRIMER_PAIR_49_PENALTY=3.995099
PRIMER_LEFT_49_PENALTY=1.478540
PRIMER_RIGHT_49_PENALTY=2.516559
PRIMER_LEFT_49_SEQUENCE=GATAGCATCCACCGAATGAA
PRIMER_RIGHT_49_SEQUENCE=GGAAGTTTACTCACTTGCGG
PRIMER_LEFT_49=657,20
PRIMER_RIGHT_49=1630,20
PRIMER_LEFT_49_TM=58.521
PRIMER_RIGHT_49_TM=57.483
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=11.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=974
=
SEQUENCE_ID=MAGIC.329_4_4626828_4627128_wil-2_edi-0_22767_65086_TRUE
SEQUENCE_TARGET=950,400
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_2_6394203_6394338_ler-0_po-0_43531_259516_TRUE
SEQUENCE_TARGET=950,235
SEQUENCE_TEMPLATE=CTATCCNGANGTNNTANNANTTCGTAATATAGCGANTNAAGNNNNNNNTNTAAATTTTCTCGCTCTTTNGTCAANNGTTTCTAAGTATATACGTCANTAGTNTCATACCACAAATCNCTGAGCCTAGTAGATNTGNCNTCCNANCCTTACCTCAANCTCTTCTCAACNNAGTATGTACATGATTNNNNNNNGTAANTAACATGTGTGAATTTCGAAAACTAAATAAACNNAAAAACATGTTGTCAAANGCCGTTTACCGTTAGTCNAACAAGAAAAATAGAATCNGAAATNGAAACGAAAACGCAAACAACATNAGNANGCAACGACTACATAACATCCNNNNNATTGAANCAACCAAAAAAAANCCTAATTTACTAATCTAAGTCTTCCTCAACTTCAAATACCCTTCTCTTTTCATGGCCNTAANAGNTCAACAACAAAACATAGACAATCATCCAAATAAANTTCCGCGGAANCAATCCATCAGAGGAAGTGAGGACCGATGAGCTTTGCAATCTCTAGGAACCCAACCCGATCTCTTCCTTCGAATATCTTCTTCAGCTTCTCATCACACAGTATTTCTCTCTTGTTTTGTGGNTCCTNAAGAACAAAATTAGTCATTTNGTAAAACNNTGATGCANGTAAAACNTCTNNAAGTACCNTAGCTAAANTGGTTCNTNAAACTTTNAANCCTCAAGNCATGTCAATGAGAATAATNNNNANCTTTCTANTANAGTAAAGTTTTGGCTACTTGGTTGCTATAGACTNTAAATCTTGCAGAGAAACAGCNTCCACATTTAAANACTATANAACTAGAATTTCAAGAGNNNNNNNNNNNNNNNTTTCATANNNNNNNAACCTGAACCAAANANCAACAATANCAAGAAGCAGAGATCAAAANATCANNNNNNNNNNNCNAATGACANAACANANTAGGATTTTGGTCAAACTAGGCAANNAGGTTCAAGAACATAGNCCAGAGAANATTATTNNTNANCTCNAAGGATACACATAGCNNNNNNNNNNNNNNNNNNNNAATCAAAGAANNNNNNTTGCTTATACAAAANGTTATAAACAACTACCCCCAAAATCATATGAAGCAATTAGTCACNANNNTANTTNCANNTTCNTAATCNNAAGAAAANTTANAAGTTACANANCTAACAAGAAGACAANCTAATTCAATTTCTGTCTTCTTNAATCCAGACCGTTCAATTTTCCTNCAGAAACCCTAAAATNTCAAACCACCNGATTGAAGAAAGCGANACAAAACCNTAAGNGAAACCAANACCAGAGTGNTTCTGAGAGAGATTNTTGGTTGTTAAGTTACCTGAAGGTCATGTTCTTTGATGTAAGCCCAAATACGCTTNAGAGCTTGTGTGCGAGCNATNTCAGGAAGNTCGACGATGTCTTGCATCTCTGGTGAAACTGGTCTAGGTTTCATGATACCGCGTGGCACTCTCTTATTGGTTGCNGTTGGCTCTGAAGATTCCGTNGCAGATGTTACCGCTCGNACCAAGCNNNGTTTAGCCGCCGGGTGANAGGCGAACCGGAGAGAGGACAGAGGAGTTGANGGGTTGCGGAAAGATGTNGTTTTGATGCAGAGGAAAGTGGANAATGTTCCAGAAGAAACCGCCATTTTCGGTGTGCTTTGTCTGTGTGGTGGTNGAATTGGGAAGNNNAAAGGGGTTTTGTGTTATCCCTTTTGCTTTTTGGTTTTTNTTTTTTTTATTCTTAGTNTTGGNGTTTTGTCTTCATCGAGGAGAAACNCTATTTATTGTCTTTTNTTTTTTCTANTGGACAAATTGTTAANAGAAAACCTTNGGGAAANTTTTGGTTAAATACAAAATTTATGCGGGTTNAAANTATAAATATTTTTAGAATNTAAAANTTTATGNNAAAAAAANTAATTGACGGTCANAAAGANNNGAANATNTAAAATTAGAANTGAAAANTTCAANAAATTTNNAATAAAANNNATAAAATNGAGANATATCNAACTAACNNAAGNNNNNNNNNNNNNNNNNNNNNNTTGNTANAATGTANNNNNNNNNNNTTTGGCCANCANTCATANANATCNTNCTCTTAAGAAGCCAAACTTNANAAGCNTAGTANAACTTTTTAAANTATGCTTC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.171631
PRIMER_LEFT_0_PENALTY=0.199316
PRIMER_RIGHT_0_PENALTY=0.972315
PRIMER_LEFT_0_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_0_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_0=486,20
PRIMER_RIGHT_0=1221,20
PRIMER_LEFT_0_TM=60.199
PRIMER_RIGHT_0_TM=59.028
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=736
PRIMER_PAIR_1_PENALTY=1.221342
PRIMER_LEFT_1_PENALTY=0.199316
PRIMER_RIGHT_1_PENALTY=1.022025
PRIMER_LEFT_1_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_1_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_1=486,20
PRIMER_RIGHT_1=1366,21
PRIMER_LEFT_1_TM=60.199
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=881
PRIMER_PAIR_2_PENALTY=1.544020
PRIMER_LEFT_2_PENALTY=0.832229
PRIMER_RIGHT_2_PENALTY=0.711791
PRIMER_LEFT_2_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_2_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_2=522,20
PRIMER_RIGHT_2=1494,20
PRIMER_LEFT_2_TM=60.832
PRIMER_RIGHT_2_TM=59.288
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=973
PRIMER_PAIR_3_PENALTY=1.546812
PRIMER_LEFT_3_PENALTY=0.199316
PRIMER_RIGHT_3_PENALTY=1.347496
PRIMER_LEFT_3_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_3_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_3=486,20
PRIMER_RIGHT_3=1366,20
PRIMER_LEFT_3_TM=60.199
PRIMER_RIGHT_3_TM=58.653
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=881
PRIMER_PAIR_4_PENALTY=1.577178
PRIMER_LEFT_4_PENALTY=0.199316
PRIMER_RIGHT_4_PENALTY=1.377862
PRIMER_LEFT_4_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_4_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_4=486,20
PRIMER_RIGHT_4=1367,21
PRIMER_LEFT_4_TM=60.199
PRIMER_RIGHT_4_TM=59.622
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=882
PRIMER_PAIR_5_PENALTY=1.670913
PRIMER_LEFT_5_PENALTY=0.959122
PRIMER_RIGHT_5_PENALTY=0.711791
PRIMER_LEFT_5_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_5_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_5=526,20
PRIMER_RIGHT_5=1494,20
PRIMER_LEFT_5_TM=60.959
PRIMER_RIGHT_5_TM=59.288
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=969
PRIMER_PAIR_6_PENALTY=1.718714
PRIMER_LEFT_6_PENALTY=0.746400
PRIMER_RIGHT_6_PENALTY=0.972315
PRIMER_LEFT_6_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_6_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_6=487,20
PRIMER_RIGHT_6=1221,20
PRIMER_LEFT_6_TM=59.254
PRIMER_RIGHT_6_TM=59.028
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=735
PRIMER_PAIR_7_PENALTY=1.718714
PRIMER_LEFT_7_PENALTY=0.746400
PRIMER_RIGHT_7_PENALTY=0.972315
PRIMER_LEFT_7_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_7_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_7=485,20
PRIMER_RIGHT_7=1221,20
PRIMER_LEFT_7_TM=59.254
PRIMER_RIGHT_7_TM=59.028
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=737
PRIMER_PAIR_8_PENALTY=1.757622
PRIMER_LEFT_8_PENALTY=1.045831
PRIMER_RIGHT_8_PENALTY=0.711791
PRIMER_LEFT_8_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_8_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_8=511,21
PRIMER_RIGHT_8=1494,20
PRIMER_LEFT_8_TM=59.954
PRIMER_RIGHT_8_TM=59.288
PRIMER_LEFT_8_GC_PERCENT=52.381
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=984
PRIMER_PAIR_9_PENALTY=1.768425
PRIMER_LEFT_9_PENALTY=0.746400
PRIMER_RIGHT_9_PENALTY=1.022025
PRIMER_LEFT_9_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_9_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_9=487,20
PRIMER_RIGHT_9=1366,21
PRIMER_LEFT_9_TM=59.254
PRIMER_RIGHT_9_TM=59.978
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=880
PRIMER_PAIR_10_PENALTY=1.768425
PRIMER_LEFT_10_PENALTY=0.746400
PRIMER_RIGHT_10_PENALTY=1.022025
PRIMER_LEFT_10_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_10_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_10=485,20
PRIMER_RIGHT_10=1366,21
PRIMER_LEFT_10_TM=59.254
PRIMER_RIGHT_10_TM=59.978
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.6000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=882
PRIMER_PAIR_11_PENALTY=1.769353
PRIMER_LEFT_11_PENALTY=0.199316
PRIMER_RIGHT_11_PENALTY=1.570037
PRIMER_LEFT_11_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_11_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_11=486,20
PRIMER_RIGHT_11=1427,21
PRIMER_LEFT_11_TM=60.199
PRIMER_RIGHT_11_TM=59.430
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=942
PRIMER_PAIR_12_PENALTY=1.773506
PRIMER_LEFT_12_PENALTY=0.801192
PRIMER_RIGHT_12_PENALTY=0.972315
PRIMER_LEFT_12_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_12_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_12=492,20
PRIMER_RIGHT_12=1221,20
PRIMER_LEFT_12_TM=60.801
PRIMER_RIGHT_12_TM=59.028
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=730
PRIMER_PAIR_13_PENALTY=1.823217
PRIMER_LEFT_13_PENALTY=0.801192
PRIMER_RIGHT_13_PENALTY=1.022025
PRIMER_LEFT_13_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_13_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_13=492,20
PRIMER_RIGHT_13=1366,21
PRIMER_LEFT_13_TM=60.801
PRIMER_RIGHT_13_TM=59.978
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=875
PRIMER_PAIR_14_PENALTY=1.854255
PRIMER_LEFT_14_PENALTY=0.832229
PRIMER_RIGHT_14_PENALTY=1.022025
PRIMER_LEFT_14_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_14_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_14=522,20
PRIMER_RIGHT_14=1366,21
PRIMER_LEFT_14_TM=60.832
PRIMER_RIGHT_14_TM=59.978
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=845
PRIMER_PAIR_15_PENALTY=1.899117
PRIMER_LEFT_15_PENALTY=1.187326
PRIMER_RIGHT_15_PENALTY=0.711791
PRIMER_LEFT_15_SEQUENCE=CAATCTCTAGGAACCCAACCC
PRIMER_RIGHT_15_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_15=512,21
PRIMER_RIGHT_15=1494,20
PRIMER_LEFT_15_TM=59.813
PRIMER_RIGHT_15_TM=59.288
PRIMER_LEFT_15_GC_PERCENT=52.381
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=983
PRIMER_PAIR_16_PENALTY=1.916583
PRIMER_LEFT_16_PENALTY=0.944268
PRIMER_RIGHT_16_PENALTY=0.972315
PRIMER_LEFT_16_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_16_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_16=483,20
PRIMER_RIGHT_16=1221,20
PRIMER_LEFT_16_TM=60.944
PRIMER_RIGHT_16_TM=59.028
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=739
PRIMER_PAIR_17_PENALTY=1.966294
PRIMER_LEFT_17_PENALTY=0.944268
PRIMER_RIGHT_17_PENALTY=1.022025
PRIMER_LEFT_17_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_17_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_17=483,20
PRIMER_RIGHT_17=1366,21
PRIMER_LEFT_17_TM=60.944
PRIMER_RIGHT_17_TM=59.978
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=884
PRIMER_PAIR_18_PENALTY=1.981148
PRIMER_LEFT_18_PENALTY=0.959122
PRIMER_RIGHT_18_PENALTY=1.022025
PRIMER_LEFT_18_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_18_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_18=526,20
PRIMER_RIGHT_18=1366,21
PRIMER_LEFT_18_TM=60.959
PRIMER_RIGHT_18_TM=59.978
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=841
PRIMER_PAIR_19_PENALTY=2.067857
PRIMER_LEFT_19_PENALTY=1.045831
PRIMER_RIGHT_19_PENALTY=1.022025
PRIMER_LEFT_19_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_19_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_19=511,21
PRIMER_RIGHT_19=1366,21
PRIMER_LEFT_19_TM=59.954
PRIMER_RIGHT_19_TM=59.978
PRIMER_LEFT_19_GC_PERCENT=52.381
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=856
PRIMER_PAIR_20_PENALTY=2.078032
PRIMER_LEFT_20_PENALTY=1.105717
PRIMER_RIGHT_20_PENALTY=0.972315
PRIMER_LEFT_20_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_20_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_20=491,20
PRIMER_RIGHT_20=1221,20
PRIMER_LEFT_20_TM=58.894
PRIMER_RIGHT_20_TM=59.028
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=731
PRIMER_PAIR_21_PENALTY=2.093896
PRIMER_LEFT_21_PENALTY=0.746400
PRIMER_RIGHT_21_PENALTY=1.347496
PRIMER_LEFT_21_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_21_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_21=487,20
PRIMER_RIGHT_21=1366,20
PRIMER_LEFT_21_TM=59.254
PRIMER_RIGHT_21_TM=58.653
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=880
PRIMER_PAIR_22_PENALTY=2.093896
PRIMER_LEFT_22_PENALTY=0.746400
PRIMER_RIGHT_22_PENALTY=1.347496
PRIMER_LEFT_22_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_22_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_22=485,20
PRIMER_RIGHT_22=1366,20
PRIMER_LEFT_22_TM=59.254
PRIMER_RIGHT_22_TM=58.653
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=882
PRIMER_PAIR_23_PENALTY=2.124261
PRIMER_LEFT_23_PENALTY=0.746400
PRIMER_RIGHT_23_PENALTY=1.377862
PRIMER_LEFT_23_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_23_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_23=487,20
PRIMER_RIGHT_23=1367,21
PRIMER_LEFT_23_TM=59.254
PRIMER_RIGHT_23_TM=59.622
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=881
PRIMER_PAIR_24_PENALTY=2.124261
PRIMER_LEFT_24_PENALTY=0.746400
PRIMER_RIGHT_24_PENALTY=1.377862
PRIMER_LEFT_24_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_24_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_24=485,20
PRIMER_RIGHT_24=1367,21
PRIMER_LEFT_24_TM=59.254
PRIMER_RIGHT_24_TM=59.622
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.6000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=883
PRIMER_PAIR_25_PENALTY=2.127742
PRIMER_LEFT_25_PENALTY=1.105717
PRIMER_RIGHT_25_PENALTY=1.022025
PRIMER_LEFT_25_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_25_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_25=491,20
PRIMER_RIGHT_25=1366,21
PRIMER_LEFT_25_TM=58.894
PRIMER_RIGHT_25_TM=59.978
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=876
PRIMER_PAIR_26_PENALTY=2.148688
PRIMER_LEFT_26_PENALTY=0.801192
PRIMER_RIGHT_26_PENALTY=1.347496
PRIMER_LEFT_26_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_26_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_26=492,20
PRIMER_RIGHT_26=1366,20
PRIMER_LEFT_26_TM=60.801
PRIMER_RIGHT_26_TM=58.653
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=875
PRIMER_PAIR_27_PENALTY=2.159641
PRIMER_LEFT_27_PENALTY=1.187326
PRIMER_RIGHT_27_PENALTY=0.972315
PRIMER_LEFT_27_SEQUENCE=CAATCTCTAGGAACCCAACCC
PRIMER_RIGHT_27_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_27=512,21
PRIMER_RIGHT_27=1221,20
PRIMER_LEFT_27_TM=59.813
PRIMER_RIGHT_27_TM=59.028
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=710
PRIMER_PAIR_28_PENALTY=2.179053
PRIMER_LEFT_28_PENALTY=0.801192
PRIMER_RIGHT_28_PENALTY=1.377862
PRIMER_LEFT_28_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_28_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_28=492,20
PRIMER_RIGHT_28=1367,21
PRIMER_LEFT_28_TM=60.801
PRIMER_RIGHT_28_TM=59.622
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=876
PRIMER_PAIR_29_PENALTY=2.179725
PRIMER_LEFT_29_PENALTY=0.832229
PRIMER_RIGHT_29_PENALTY=1.347496
PRIMER_LEFT_29_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_29_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_29=522,20
PRIMER_RIGHT_29=1366,20
PRIMER_LEFT_29_TM=60.832
PRIMER_RIGHT_29_TM=58.653
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=845
PRIMER_PAIR_30_PENALTY=2.209351
PRIMER_LEFT_30_PENALTY=1.187326
PRIMER_RIGHT_30_PENALTY=1.022025
PRIMER_LEFT_30_SEQUENCE=CAATCTCTAGGAACCCAACCC
PRIMER_RIGHT_30_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_30=512,21
PRIMER_RIGHT_30=1366,21
PRIMER_LEFT_30_TM=59.813
PRIMER_RIGHT_30_TM=59.978
PRIMER_LEFT_30_GC_PERCENT=52.381
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.4000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=855
PRIMER_PAIR_31_PENALTY=2.210091
PRIMER_LEFT_31_PENALTY=0.832229
PRIMER_RIGHT_31_PENALTY=1.377862
PRIMER_LEFT_31_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_31_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_31=522,20
PRIMER_RIGHT_31=1367,21
PRIMER_LEFT_31_TM=60.832
PRIMER_RIGHT_31_TM=59.622
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.7000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=846
PRIMER_PAIR_32_PENALTY=2.233540
PRIMER_LEFT_32_PENALTY=1.261226
PRIMER_RIGHT_32_PENALTY=0.972315
PRIMER_LEFT_32_SEQUENCE=AAGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_32_SEQUENCE=AGGAAAATTGAACGGTCTGG
PRIMER_LEFT_32=490,21
PRIMER_RIGHT_32=1221,20
PRIMER_LEFT_32_TM=60.261
PRIMER_RIGHT_32_TM=59.028
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=732
PRIMER_PAIR_33_PENALTY=2.283251
PRIMER_LEFT_33_PENALTY=1.261226
PRIMER_RIGHT_33_PENALTY=1.022025
PRIMER_LEFT_33_SEQUENCE=AAGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_33_SEQUENCE=GCGTATTTGGGCTTACATCAA
PRIMER_LEFT_33=490,21
PRIMER_RIGHT_33=1366,21
PRIMER_LEFT_33_TM=60.261
PRIMER_RIGHT_33_TM=59.978
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=877
PRIMER_PAIR_34_PENALTY=2.291764
PRIMER_LEFT_34_PENALTY=0.944268
PRIMER_RIGHT_34_PENALTY=1.347496
PRIMER_LEFT_34_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_34_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_34=483,20
PRIMER_RIGHT_34=1366,20
PRIMER_LEFT_34_TM=60.944
PRIMER_RIGHT_34_TM=58.653
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.6000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=884
PRIMER_PAIR_35_PENALTY=2.306618
PRIMER_LEFT_35_PENALTY=0.959122
PRIMER_RIGHT_35_PENALTY=1.347496
PRIMER_LEFT_35_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_35_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_35=526,20
PRIMER_RIGHT_35=1366,20
PRIMER_LEFT_35_TM=60.959
PRIMER_RIGHT_35_TM=58.653
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=841
PRIMER_PAIR_36_PENALTY=2.316437
PRIMER_LEFT_36_PENALTY=0.746400
PRIMER_RIGHT_36_PENALTY=1.570037
PRIMER_LEFT_36_SEQUENCE=AGGAAGTGAGGACCGATGAG
PRIMER_RIGHT_36_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_36=487,20
PRIMER_RIGHT_36=1427,21
PRIMER_LEFT_36_TM=59.254
PRIMER_RIGHT_36_TM=59.430
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=941
PRIMER_PAIR_37_PENALTY=2.316437
PRIMER_LEFT_37_PENALTY=0.746400
PRIMER_RIGHT_37_PENALTY=1.570037
PRIMER_LEFT_37_SEQUENCE=AGAGGAAGTGAGGACCGATG
PRIMER_RIGHT_37_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_37=485,20
PRIMER_RIGHT_37=1427,21
PRIMER_LEFT_37_TM=59.254
PRIMER_RIGHT_37_TM=59.430
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=42.857
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.6000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=943
PRIMER_PAIR_38_PENALTY=2.316463
PRIMER_LEFT_38_PENALTY=0.199316
PRIMER_RIGHT_38_PENALTY=2.117147
PRIMER_LEFT_38_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_38_SEQUENCE=TTCACCAGAGATGCAAGACA
PRIMER_LEFT_38=486,20
PRIMER_RIGHT_38=1426,20
PRIMER_LEFT_38_TM=60.199
PRIMER_RIGHT_38_TM=57.883
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.6000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=941
PRIMER_PAIR_39_PENALTY=2.322130
PRIMER_LEFT_39_PENALTY=0.944268
PRIMER_RIGHT_39_PENALTY=1.377862
PRIMER_LEFT_39_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_39_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_39=483,20
PRIMER_RIGHT_39=1367,21
PRIMER_LEFT_39_TM=60.944
PRIMER_RIGHT_39_TM=59.622
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=885
PRIMER_PAIR_40_PENALTY=2.336984
PRIMER_LEFT_40_PENALTY=0.959122
PRIMER_RIGHT_40_PENALTY=1.377862
PRIMER_LEFT_40_SEQUENCE=CCAACCCGATCTCTTCCTTC
PRIMER_RIGHT_40_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_40=526,20
PRIMER_RIGHT_40=1367,21
PRIMER_LEFT_40_TM=60.959
PRIMER_RIGHT_40_TM=59.622
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=842
PRIMER_PAIR_41_PENALTY=2.371228
PRIMER_LEFT_41_PENALTY=0.801192
PRIMER_RIGHT_41_PENALTY=1.570037
PRIMER_LEFT_41_SEQUENCE=GTGAGGACCGATGAGCTTTG
PRIMER_RIGHT_41_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_41=492,20
PRIMER_RIGHT_41=1427,21
PRIMER_LEFT_41_TM=60.801
PRIMER_RIGHT_41_TM=59.430
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=936
PRIMER_PAIR_42_PENALTY=2.393327
PRIMER_LEFT_42_PENALTY=1.045831
PRIMER_RIGHT_42_PENALTY=1.347496
PRIMER_LEFT_42_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_42_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_42=511,21
PRIMER_RIGHT_42=1366,20
PRIMER_LEFT_42_TM=59.954
PRIMER_RIGHT_42_TM=58.653
PRIMER_LEFT_42_GC_PERCENT=52.381
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=856
PRIMER_PAIR_43_PENALTY=2.402266
PRIMER_LEFT_43_PENALTY=0.832229
PRIMER_RIGHT_43_PENALTY=1.570037
PRIMER_LEFT_43_SEQUENCE=GAACCCAACCCGATCTCTTC
PRIMER_RIGHT_43_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_43=522,20
PRIMER_RIGHT_43=1427,21
PRIMER_LEFT_43_TM=60.832
PRIMER_RIGHT_43_TM=59.430
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=906
PRIMER_PAIR_44_PENALTY=2.414089
PRIMER_LEFT_44_PENALTY=1.702298
PRIMER_RIGHT_44_PENALTY=0.711791
PRIMER_LEFT_44_SEQUENCE=TGCAATCTCTAGGAACCCAA
PRIMER_RIGHT_44_SEQUENCE=ACGGAATCTTCAGAGCCAAC
PRIMER_LEFT_44=510,20
PRIMER_RIGHT_44=1494,20
PRIMER_LEFT_44_TM=58.298
PRIMER_RIGHT_44_TM=59.288
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=10.0000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=985
PRIMER_PAIR_45_PENALTY=2.423693
PRIMER_LEFT_45_PENALTY=1.045831
PRIMER_RIGHT_45_PENALTY=1.377862
PRIMER_LEFT_45_SEQUENCE=GCAATCTCTAGGAACCCAACC
PRIMER_RIGHT_45_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_45=511,21
PRIMER_RIGHT_45=1367,21
PRIMER_LEFT_45_TM=59.954
PRIMER_RIGHT_45_TM=59.622
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=857
PRIMER_PAIR_46_PENALTY=2.453213
PRIMER_LEFT_46_PENALTY=1.105717
PRIMER_RIGHT_46_PENALTY=1.347496
PRIMER_LEFT_46_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_46_SEQUENCE=GCGTATTTGGGCTTACATCA
PRIMER_LEFT_46=491,20
PRIMER_RIGHT_46=1366,20
PRIMER_LEFT_46_TM=58.894
PRIMER_RIGHT_46_TM=58.653
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=876
PRIMER_PAIR_47_PENALTY=2.483579
PRIMER_LEFT_47_PENALTY=1.105717
PRIMER_RIGHT_47_PENALTY=1.377862
PRIMER_LEFT_47_SEQUENCE=AGTGAGGACCGATGAGCTTT
PRIMER_RIGHT_47_SEQUENCE=AGCGTATTTGGGCTTACATCA
PRIMER_LEFT_47=491,20
PRIMER_RIGHT_47=1367,21
PRIMER_LEFT_47_TM=58.894
PRIMER_RIGHT_47_TM=59.622
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=877
PRIMER_PAIR_48_PENALTY=2.498214
PRIMER_LEFT_48_PENALTY=0.199316
PRIMER_RIGHT_48_PENALTY=2.298898
PRIMER_LEFT_48_SEQUENCE=GAGGAAGTGAGGACCGATGA
PRIMER_RIGHT_48_SEQUENCE=GTTTCACCAGAGATGCAAGACA
PRIMER_LEFT_48=486,20
PRIMER_RIGHT_48=1428,22
PRIMER_LEFT_48_TM=60.199
PRIMER_RIGHT_48_TM=60.299
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.6000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=943
PRIMER_PAIR_49_PENALTY=2.514305
PRIMER_LEFT_49_PENALTY=0.944268
PRIMER_RIGHT_49_PENALTY=1.570037
PRIMER_LEFT_49_SEQUENCE=TCAGAGGAAGTGAGGACCGA
PRIMER_RIGHT_49_SEQUENCE=TTTCACCAGAGATGCAAGACA
PRIMER_LEFT_49=483,20
PRIMER_RIGHT_49=1427,21
PRIMER_LEFT_49_TM=60.944
PRIMER_RIGHT_49_TM=59.430
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=945
=
SEQUENCE_ID=MAGIC.338_2_5741683_5742379_po-0_ct-1_80855_30182_TRUE
SEQUENCE_TARGET=950,796
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGGTGAGTACTCCTCTCGCNATCATCAGTCTTTACTCTGTTCCAAGTCTTCCTTCTTTGGCTCAAATGGGGTCCACGNTNATCAANCCAAANAGATNCGACTTTTTNNNCTGGGGTTTCGTCGAGAATTCGATTTTNGGGTTTCNTTCGAAACTTGGTCAAATGTCGAATACGCGTTACNAAGAACAAACCTTTNTCTTTATTTCNTTTGTCTTCGTCAACNGCTGAATCAACCAAANGGTCCCTGGAATNAATAAACCTCTAATAATAATGGCTTTGCTTTTACTCTGATGNCAAGTTCAAAAATGGAACAAAGATCACTCCTTTGCTTCCTTTATCTNCTNCTNCTATTCAATTTCACTCTCAGAGTCGCTGGAAACGCTGAAGGTTCTTCNTTTNTNTGCTACTCTCATGAAAAGTCCCTTCNTTTNTGGTNGTTGTTAGATTTNGAAAGTTCNTANCTTTNTTNNNNNNNNNNNNNNNNNNNNNTATGAATNNGTGTACAGGTGATGCTTTGACTCAGCTGAANAACAGTTTNTCATCAGGTGACCCNGCAAACAATGTACTCCAAAGCTGGGATGCTACTCTTGTTACTCCATGTACTTGGTTTCATGTTACTTGCAATCCTGAGAATAAAGTTACTCGTGTGTATGTTTCTCTNNNNNNNNNNNNNNNNNNNNNNNNNNTGCTNGTTTTNTCTGAATGAANGNTTCAANAATNCCTTTGNNAATNTACTGTTTGATGTTTGNTTAGTGACCTTGGGAATGCAAAACTATCTGGAAAGTTGGTNCCAGANCTTGGTCAGCTTTNAAACTTGCAGTANTTGTAAGCCATCTCTTCCTCAATGTCNATTAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNTNNGTNTNNNCTTTCNAAAGAGAATTGTGGATAAACAANTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATTTTAGATCTCTTTAGCTAGGCTTCTCGAGCCTTGTTACTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATTAGATAAGTANCTNGAGAGTTTGGATCNGGTATTTCTCTACAGGTGATCTCAACTCTNCATATTTGTGNTNCNGCTGCNAAATGTTTTNTTGAAANTATTGCTTACANATATAGTTTCCACNTTCATGTANNNNNNNNNTCTACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATACTTCATTANGTTTTCTGTANCNTNAANTTCTGGTAAAATANNNNTAGAGATTTTCCANTCNACAATNATTAANCAAANNNNNNNNNNNNNNNNGCTTAATGACAGGGAGCTTTANAGCAATAACATTACNGGGGAGATACCTGAGGAGCTTGGNGACTTGGTGGAACTAGTAAGCTTGGATCTTTACGCNAACANCATAAGCGGTCCCATCCCTTCNTCNCTTGGCAAACTAGGAAAACTNCGNTTCTTGTATGNANNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4739079_4739625_ler-0_can-0_16546_2734_TRUE
SEQUENCE_TARGET=950,646
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17379630_17379888_tsu-0_po-0_6905_12540_TRUE
SEQUENCE_TARGET=950,358
SEQUENCE_TEMPLATE=ATNCNAACAAATAGTTGAGGCCAGATCATTNATCTAATTTGTGTTTTNNNNNNNNNNNNNNNNNNNAAAAAANAANNATCAAGATTNTAGGGGGNAANNNCAAACCTGGACATCAAACTCAATGAAGTCAATAGGANAATCTGCAGCAACATTAAAAGAAAGAAGAGAATTTTCTTTGATAAANTTCATTTTCTCGTCCGGAGATTGAAGCATGTTCANCCCAAATCCTCTATGACCCATCAAAACAAATTTAGGGAAAACAANNGCCTCTTGCTTCTTATCATCTTCAACAACACCTTNAAAANCNACACAAAACCAATCATAACCAATAATATATTAACAAAAAAAANNANAANTTTTTGCATGGAGCCATTTGNTATTATTACCTGATGATAACATGGCAGAANTAGATAAAGACAATGTCATAGTTTCAAGAGCCATGGTTTAGTTNCTTGNGAACAAAGATTTATATTAAAGAANTGGGTTTAAGATNATATGNGCAGGACNAAAGAATATTCCATTCGATACAAAAGAAGAATGCTCTAGATATCGAATGGAATACTCCATTCGCAATAATCTTCAAGNTTTTCTTTTTGTTGGCTCTAAAAGAACTTTTCNTGTTGAATTTTATTCTATCTGATTTTTNGANANCTTTTAATTNTCTGGATCATTCTNTTTNGNTCTATTTATAAGACAGATGGANAGGGTTTAATTTAGNCAAGTAGACANAACTACACGTGAATAAAAATAAANTTTNTTTTCGTTTNGGGGTTTTNTTAGTTANTTTAGGTNAATTCTTCTNTTCATATTTNTGGATNNTTNTTTTNTTCCAAAAACATCTACANGATTAATTTTTAGATTACANANTTTTTTGTTAATNAAGATAGAACCGCAAACTTCTGTNTNGNTAGTAGATTGCTATNANTNATGNACCANATGAAGTCTTATAGGNAGNNNNNAAANAAAANANANTTCNTTGCATTGGAACCAAATGTTTGNANCAANTGTAGTGTTTTNNAGACATTGTTGGAGAAGAGGCTCGATATTGNATACATGATAAATACTCTNTCACTAAAAGATTTCAAAGCTTATCTATATTGTGTACTCAGGATACTGTCTTCTTGTTCTTNAANGGAGACAGNAANGAAAAATGGCGAGAAGCTGNAAAANANAANANNNAGAAGAAAANTGAACAAAAGTGAGCNAANTTTGATTAGANTCAAANAAGTTTCTNNGNNTNAAAAATATNTTGTTCACTNACATTTCTGTGAAGCANCNGCTGCTGTAGGTGTTTCNTCNTGAGATATGAGATTCTTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTNAAACACCAACAGAACCATATTGATGTGNAAAANAAGGTTCTTGNTGTTATGGGATAANGTTAAATCCAAGAGNAAANAATTNAGTGGNACAAACTCCTNAATATCTCTNANAAGAAAGCAAACNNNNNNNNNNNNNNNNNNNNNNGAGTAACTACTGTAAAAGATAAAAGGATGTAACAAGGATTTACCTTCACTTGTCGTGGCAGCAGTTACAGCTGGTTCATGATCCTCANTGCTATTAAAAGATGCATTATTAGACTCGGTATGGTGTTCACTTACAGAAGAAGANTGACTCTTCTTTCCAAATTTCAACAGTTTTCTGAGCCCTTTTGACGCTGACTCCTTCGTTTGAGATTTCTCCGNGGCCTCACTTATCTTCTTCTCTGGTACAAGGACTTTGACAGTTTCTTGCTCTGTCTCATTTGAATATAAGCTTGGTGGAGGTGCTTGGGAACAGTCNGAGTACACTGTACATGGATCTTCTAACGAAGAAACCCGAGCATATGGAGCTTCATATGGTTTNGCAACAATACTTTGACTTGTCAGAAACTTTGGTGTTTCTGTTACNAAGTCACTCAGANTNTCCAATCTAACCTGAAAGCAAAATAANCATTCCTTTCATCAAAACCGTTGAAANAAAAATTAAAATTGACTCAGCTAGAATAAGANTACTTACTAAGACTAGATCATTGCCACNCTCCTGCATTGTTTNNNCAATGNNTTCNTTNTCAACTCCTTCTCGAATGGTGGAGTTTTCAGAGACTACATCAGACTTNTCGTNTTTTACCNGATCTCTACTTATACTTCGAGCTGAACTTGGCAACACCATCACTACTGTCTTCTCAACCACTGGAGTTTCATCAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_15691715_15691717_zu-0_oy-0_38943_178979_TRUE
SEQUENCE_TARGET=950,102
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_14469454_14470166_wil-2_sf-2_11119_2891_TRUE
SEQUENCE_TARGET=950,812
SEQUENCE_TEMPLATE=ATGNTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANANTANAATTCAGGATGATGNNGCTGATAANAATCAATTGAGGANAAAGAGGAAGNGNAAAGTTNTTGACGNTTGCCCCNNGAAAGTNNNGACNTATCCNGAACAAGAAAAAAACAGTNGGAGTTTNGTCAAGTTTNNATTTTAANNAACTTAAGATTTTTGGTGTTGAATGAGTNAGAACNTGTTGAAGAAGACCTGGAAAATGANAAAAGAGAGGACNANATANAAGGTTTGGAAGAAGAGGANNANCTNGAAGATAAAGTANANGATAAAANAAGGNNGGGNNNTGTAACAGAGATGGANAAGGNTAATAAGGATGCANANATAACTCTNAANATGNAANTCAAANAGGNAAAGTCGCTCCNNCAAGNCCATCACTACACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTGCAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_2579586_2579629_can-0_kn-0_2922_4787_TRUE
SEQUENCE_TARGET=950,143
SEQUENCE_TEMPLATE=GGTTTTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNCNNNNNNNNNNNNNNNTGAGTCCTTCCNANGCCCANNNTTTCTGAAATTGAAGTGAGTTTCNTCCNNAATCCCTTAAAGTTTCAAANTTTTCAATNAACNTNTNNNTNTTTNTCTTGTTTNTNTCCGAATCGTNTGAGNGTTTTNATTTTCTATNCTCTGANTTGGTCTCANTCGATGTACGTTTNTAANGAATGNGTGTTCNAAATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATGAGGTACCAATTNACNGTCTTTGANTCTTTCNTNNTTGTTTTGTNATNAAGGGTTCTGAGCTTTGTNTCTTTAGGTTAATTTTCTATNTTNTNTNNNNNNNNNNNNNNNNNNNNNTAGCCAAACCAAAGANTGTGNNATCTGNATTTTNATTTNNGTNAAAAANACTGTATAGAAATGNCTTACNCTCAAGNAACTTTTATATTGCNTAAATGANTNGCTNAGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAGTNTTTTNGGACANAGATGGCANNANTTCAGAAACCAGTGNTNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGCAAANACNTGCGTTTACNGAACAGGTNNNNTATANAAGTGTTCATGTATNTCTTTCTAATANTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGAATTACNTTTCTNGTTTGAGANAANGTCATNTTNGANNTGGTNAAGNNNNTAAGAACGTTGACATNTTNTGNTTNAAAANAAAAGACCTGGCTTNCATTAACTNTGCNTTGGANGCTTNCTCTAATTANTTNCTGATTAAAGAAAGACATNCCCTTANTNTNNAGNTGACCATTANACAGAACAGTAAGCTCTNNNCACNCACATGGNNNNNNNNNNNNATNNAAACGGACTAACTTGTANTCTCTNTNCCCTGTCAAGNNTTAGCAAATCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCTNTCTCTGNCCCCTCTTCTTGNTNTCANTGACNNTTNTTTTNGGTGNTTGNNNNNNTTANNNNTGTGNTTTCAAANNANNNNNNTGTNATGATNNTAGGNTTNTGATGGNNNNNNNNNNNNNNNNNCTNGATANNANTGAGGCANAGANGTCAACNGNTGTGTNTCATGACCAANNGNNANNAANANACTTCCCNATGAATNTGANAANCAAAGTCGCANGCAAACTCNGTTCNCNAGGAGGNGATNGCGCCACNNCACCTANGGNAGACANTNAATCTGGNCTAGNGTTCCNNAACAANCTCANATTNTCNGATCTTTTCNATNTCCCNNNTGAAAATGTCCANATGAATNNNNNNNNNTTTAGCGGANTGANTNATGGGNACAAAGANTGTCTGGANANNNNNNNNNNNNNTGGAACNNNAATGGCATGTCTNAAGAGCGATNNAGGAGATTCNNTNCTTCATCTTGCTGCTANATGGNGTCATCTAGAANTAGTNAAGANCNTTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGNNTCAGNTTCCNCTTCATGTGGCNGCTCNTGCNNNNNNNNNNNNNNNNNNNNANGCTCTTGTTGCNNCNNTAANATTTNTNTCAGATAGACTGGCTGAAGAAGNTANNGAGAGACTNAATCCATATGTTCTCNNNGANAAANATGGAAATANTNCTCTGCACTTGGCCATTGNAGGACGCTANNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCANNATGCTTCTTTTCTTGANAATAANNANGGAATATCTNCCTTGTANATGGCNGTAGAANCNGGAGANNTNNCNCTNGTG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_2_502643_502650_hi-0_edi-0_198311_115695_TRUE
SEQUENCE_TARGET=950,107
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_18496238_18496336_mt-0_po-0_9911_25223_TRUE
SEQUENCE_TARGET=950,198
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGATCTCAAANATAAGNGGTGGACTCACGACNATTGATGGAGAANNTTGAGGAGTTGACTAATCCCCTCCATGAGATACATGCTGNAGGGAGGAGACTATCAAGNNNNTNTTTTTAATTGAAAATTTCGTTATGGCTGGACCAANNCCAAAACTATTTCAAGNTTGAAGCAAAAGTGGATNAGTTTTCAAGACTNGATNTTTANGCTCCTNGTTNAGTCAANGAACCGGAGACAAATAGNAGTTGGTGTACNAATTNGCAAATTNAACNCGAATATNAGNATCAAACCCAAAAACAAGGANANTGGCAAACAAGGGANANACTCCNNNTTGTTTTCCTTGTTTNCCATTTTCCTTGTTTTTTGGACCCAAATTTTAGTCATTTTNGCTTCCCNACNANCTATCTANTATCTATACTTCAACTTGTAAAAATCACCATTTTATGAATTTAAGACAGAACATAACNCAACNAGNTCCAAANAGAAAAANTAGTAATCATTTACTCAAAAATGTAATAANATTAGNGTGTTAATNTGAAGANAGNTTCNCATCTACTATGCTCCTTCAATCAAAATTAGNAGTTATTTACGCTCAAAAAAGAGAANTNAATGAACTCAACAACACCGATAATGTATGTGTTCATATAGTATTGTCACCTANGAAGGCGAAAACCCGTTTCTTGAAAATGCGNGCTAAATCCATGATTTCGGTATTCCTCATNCTCTCAACACTGCTTTCAGGTTNTCCAAGTAAGTTTCTTTTACGGGATAATCTTGTGGCTGCATCAACCGTTAGACTCGCCATGTCTCTGCTNGTTGTTAGTTCCTTTAGAATAGTAGCAGAGCTTGACAAGCCAGANACAAGTCTGTTTAGTAATATCAATATCTCTCTCATCAATAGTGTTCTGCGCCAGTAAAANAAATANATTTTTGTAAGTCANTAGNNTAAANTTTNNATTTGTAAATCACCTCGGTGTAGAATAAACCTNGCTTTGAAGATTTCCGCTTTTGGATGAAATTCTGTTGATTCCGAGTCTATTTCNGCAACTAAAAGATGAAGTATCAGCANTAAGAAGCTGCTGTCTTGAGGTAGCTTGTGACTTGAGAGTAGTTCATAGCCAGAGTTTCCTGAAGAAGCTGCTAGAGCCAAGATCATTATTACATTCCTACANAGTTCCAGATCCTGAAANTCAAATNAANCGAAAGCTGTGTTAAATAATCCAATTGCAGTACACTACTTAACACNANGGNTTTTTTTCCGGTTTTTTAATTACCTCTGATGTCTTTCTGGGAGAAGTCAAACAATCTGCTAAGCCTTCAAAGATCTTACCAAATGCTTCCAAAGCAANGAAATTTNCTTCACTATCGTTTTCAGTATCAGAGGAGTTCTTTTCCTCATGGAGAGAATCAAATGTATCGTATAGTTTAGGACCTTTGAACCNAAAATAAAGAATATATTAGAGAAGAAAGAAACGCAACTTTGGCGAAGATGGCTTCATCATAAAACNAAGAAATATGATCTTCAAGTAAGTACNTACAGTTGAGTAGCAAGTAAAAAAGATGAATAGCTTCTTTCCGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.195910
PRIMER_LEFT_0_PENALTY=3.179598
PRIMER_RIGHT_0_PENALTY=0.016312
PRIMER_LEFT_0_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_0_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_0=582,20
PRIMER_RIGHT_0=1539,20
PRIMER_LEFT_0_TM=56.820
PRIMER_RIGHT_0_TM=60.016
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.6000
PRIMER_RIGHT_0_END_STABILITY=11.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=958
PRIMER_PAIR_1_PENALTY=3.415966
PRIMER_LEFT_1_PENALTY=3.399655
PRIMER_RIGHT_1_PENALTY=0.016312
PRIMER_LEFT_1_SEQUENCE=GAGTTGACTAATCCCCTCCA
PRIMER_RIGHT_1_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_1=573,20
PRIMER_RIGHT_1=1539,20
PRIMER_LEFT_1_TM=56.600
PRIMER_RIGHT_1_TM=60.016
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=967
PRIMER_PAIR_2_PENALTY=3.456411
PRIMER_LEFT_2_PENALTY=3.179598
PRIMER_RIGHT_2_PENALTY=0.276812
PRIMER_LEFT_2_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_2_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_2=582,20
PRIMER_RIGHT_2=1327,20
PRIMER_LEFT_2_TM=56.820
PRIMER_RIGHT_2_TM=60.277
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=746
PRIMER_PAIR_3_PENALTY=3.676467
PRIMER_LEFT_3_PENALTY=3.399655
PRIMER_RIGHT_3_PENALTY=0.276812
PRIMER_LEFT_3_SEQUENCE=GAGTTGACTAATCCCCTCCA
PRIMER_RIGHT_3_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_3=573,20
PRIMER_RIGHT_3=1327,20
PRIMER_LEFT_3_TM=56.600
PRIMER_RIGHT_3_TM=60.277
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=755
PRIMER_PAIR_4_PENALTY=3.836692
PRIMER_LEFT_4_PENALTY=3.179598
PRIMER_RIGHT_4_PENALTY=0.657094
PRIMER_LEFT_4_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_4_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_4=582,20
PRIMER_RIGHT_4=1321,20
PRIMER_LEFT_4_TM=56.820
PRIMER_RIGHT_4_TM=60.657
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=740
PRIMER_PAIR_5_PENALTY=3.941762
PRIMER_LEFT_5_PENALTY=3.284668
PRIMER_RIGHT_5_PENALTY=0.657094
PRIMER_LEFT_5_SEQUENCE=AAATTTCGTTATGGCTGGAC
PRIMER_RIGHT_5_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_5=643,20
PRIMER_RIGHT_5=1321,20
PRIMER_LEFT_5_TM=56.715
PRIMER_RIGHT_5_TM=60.657
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=679
PRIMER_PAIR_6_PENALTY=3.996800
PRIMER_LEFT_6_PENALTY=3.980488
PRIMER_RIGHT_6_PENALTY=0.016312
PRIMER_LEFT_6_SEQUENCE=CTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_6_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_6=580,22
PRIMER_RIGHT_6=1539,20
PRIMER_LEFT_6_TM=58.020
PRIMER_RIGHT_6_TM=60.016
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.6000
PRIMER_RIGHT_6_END_STABILITY=11.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=960
PRIMER_PAIR_7_PENALTY=4.021140
PRIMER_LEFT_7_PENALTY=3.179598
PRIMER_RIGHT_7_PENALTY=0.841542
PRIMER_LEFT_7_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_7_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_7=582,20
PRIMER_RIGHT_7=1540,20
PRIMER_LEFT_7_TM=56.820
PRIMER_RIGHT_7_TM=59.158
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=11.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=959
PRIMER_PAIR_8_PENALTY=4.056748
PRIMER_LEFT_8_PENALTY=3.399655
PRIMER_RIGHT_8_PENALTY=0.657094
PRIMER_LEFT_8_SEQUENCE=GAGTTGACTAATCCCCTCCA
PRIMER_RIGHT_8_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_8=573,20
PRIMER_RIGHT_8=1321,20
PRIMER_LEFT_8_TM=56.600
PRIMER_RIGHT_8_TM=60.657
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=749
PRIMER_PAIR_9_PENALTY=4.061238
PRIMER_LEFT_9_PENALTY=4.044927
PRIMER_RIGHT_9_PENALTY=0.016312
PRIMER_LEFT_9_SEQUENCE=TAATCCCCTCCATGAGATACA
PRIMER_RIGHT_9_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_9=581,21
PRIMER_RIGHT_9=1539,20
PRIMER_LEFT_9_TM=56.955
PRIMER_RIGHT_9_TM=60.016
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.6000
PRIMER_RIGHT_9_END_STABILITY=11.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=959
PRIMER_PAIR_10_PENALTY=4.126210
PRIMER_LEFT_10_PENALTY=3.284668
PRIMER_RIGHT_10_PENALTY=0.841542
PRIMER_LEFT_10_SEQUENCE=AAATTTCGTTATGGCTGGAC
PRIMER_RIGHT_10_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_10=643,20
PRIMER_RIGHT_10=1540,20
PRIMER_LEFT_10_TM=56.715
PRIMER_RIGHT_10_TM=59.158
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=11.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=898
PRIMER_PAIR_11_PENALTY=4.156401
PRIMER_LEFT_11_PENALTY=4.140089
PRIMER_RIGHT_11_PENALTY=0.016312
PRIMER_LEFT_11_SEQUENCE=ACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_11_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_11=579,23
PRIMER_RIGHT_11=1539,20
PRIMER_LEFT_11_TM=58.860
PRIMER_RIGHT_11_TM=60.016
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=5.6000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=961
PRIMER_PAIR_12_PENALTY=4.182411
PRIMER_LEFT_12_PENALTY=3.179598
PRIMER_RIGHT_12_PENALTY=1.002812
PRIMER_LEFT_12_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_12_SEQUENCE=TGCAGCCACAAGATTATCCC
PRIMER_LEFT_12=582,20
PRIMER_RIGHT_12=1303,20
PRIMER_LEFT_12_TM=56.820
PRIMER_RIGHT_12_TM=61.003
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=5.6000
PRIMER_RIGHT_12_END_STABILITY=9.3000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=722
PRIMER_PAIR_13_PENALTY=4.241196
PRIMER_LEFT_13_PENALTY=3.399655
PRIMER_RIGHT_13_PENALTY=0.841542
PRIMER_LEFT_13_SEQUENCE=GAGTTGACTAATCCCCTCCA
PRIMER_RIGHT_13_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_13=573,20
PRIMER_RIGHT_13=1540,20
PRIMER_LEFT_13_TM=56.600
PRIMER_RIGHT_13_TM=59.158
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=11.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=968
PRIMER_PAIR_14_PENALTY=4.257300
PRIMER_LEFT_14_PENALTY=3.980488
PRIMER_RIGHT_14_PENALTY=0.276812
PRIMER_LEFT_14_SEQUENCE=CTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_14_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_14=580,22
PRIMER_RIGHT_14=1327,20
PRIMER_LEFT_14_TM=58.020
PRIMER_RIGHT_14_TM=60.277
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=5.6000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=748
PRIMER_PAIR_15_PENALTY=4.287481
PRIMER_LEFT_15_PENALTY=3.284668
PRIMER_RIGHT_15_PENALTY=1.002812
PRIMER_LEFT_15_SEQUENCE=AAATTTCGTTATGGCTGGAC
PRIMER_RIGHT_15_SEQUENCE=TGCAGCCACAAGATTATCCC
PRIMER_LEFT_15=643,20
PRIMER_RIGHT_15=1303,20
PRIMER_LEFT_15_TM=56.715
PRIMER_RIGHT_15_TM=61.003
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=9.3000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=661
PRIMER_PAIR_16_PENALTY=4.321739
PRIMER_LEFT_16_PENALTY=4.044927
PRIMER_RIGHT_16_PENALTY=0.276812
PRIMER_LEFT_16_SEQUENCE=TAATCCCCTCCATGAGATACA
PRIMER_RIGHT_16_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_16=581,21
PRIMER_RIGHT_16=1327,20
PRIMER_LEFT_16_TM=56.955
PRIMER_RIGHT_16_TM=60.277
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=5.6000
PRIMER_RIGHT_16_END_STABILITY=9.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=747
PRIMER_PAIR_17_PENALTY=4.402467
PRIMER_LEFT_17_PENALTY=3.399655
PRIMER_RIGHT_17_PENALTY=1.002812
PRIMER_LEFT_17_SEQUENCE=GAGTTGACTAATCCCCTCCA
PRIMER_RIGHT_17_SEQUENCE=TGCAGCCACAAGATTATCCC
PRIMER_LEFT_17=573,20
PRIMER_RIGHT_17=1303,20
PRIMER_LEFT_17_TM=56.600
PRIMER_RIGHT_17_TM=61.003
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=731
PRIMER_PAIR_18_PENALTY=4.416901
PRIMER_LEFT_18_PENALTY=4.140089
PRIMER_RIGHT_18_PENALTY=0.276812
PRIMER_LEFT_18_SEQUENCE=ACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_18_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_18=579,23
PRIMER_RIGHT_18=1327,20
PRIMER_LEFT_18_TM=58.860
PRIMER_RIGHT_18_TM=60.277
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=5.6000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=749
PRIMER_PAIR_19_PENALTY=4.576235
PRIMER_LEFT_19_PENALTY=4.559923
PRIMER_RIGHT_19_PENALTY=0.016312
PRIMER_LEFT_19_SEQUENCE=GACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_19_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_19=578,24
PRIMER_RIGHT_19=1539,20
PRIMER_LEFT_19_TM=60.560
PRIMER_RIGHT_19_TM=60.016
PRIMER_LEFT_19_GC_PERCENT=45.833
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=11.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=962
PRIMER_PAIR_20_PENALTY=4.576235
PRIMER_LEFT_20_PENALTY=4.559923
PRIMER_RIGHT_20_PENALTY=0.016312
PRIMER_LEFT_20_SEQUENCE=TGACTAATCCCCTCCATGAGATAC
PRIMER_RIGHT_20_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_20=577,24
PRIMER_RIGHT_20=1539,20
PRIMER_LEFT_20_TM=60.560
PRIMER_RIGHT_20_TM=60.016
PRIMER_LEFT_20_GC_PERCENT=45.833
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=5.3000
PRIMER_RIGHT_20_END_STABILITY=11.4000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=963
PRIMER_PAIR_21_PENALTY=4.599518
PRIMER_LEFT_21_PENALTY=3.179598
PRIMER_RIGHT_21_PENALTY=1.419920
PRIMER_LEFT_21_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_21_SEQUENCE=TTTTACTGGCGCAGAACACT
PRIMER_LEFT_21=582,20
PRIMER_RIGHT_21=1437,20
PRIMER_LEFT_21_TM=56.820
PRIMER_RIGHT_21_TM=58.580
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=5.6000
PRIMER_RIGHT_21_END_STABILITY=6.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=856
PRIMER_PAIR_22_PENALTY=4.637582
PRIMER_LEFT_22_PENALTY=3.980488
PRIMER_RIGHT_22_PENALTY=0.657094
PRIMER_LEFT_22_SEQUENCE=CTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_22_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_22=580,22
PRIMER_RIGHT_22=1321,20
PRIMER_LEFT_22_TM=58.020
PRIMER_RIGHT_22_TM=60.657
PRIMER_LEFT_22_GC_PERCENT=45.455
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=5.6000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=742
PRIMER_PAIR_23_PENALTY=4.702020
PRIMER_LEFT_23_PENALTY=4.044927
PRIMER_RIGHT_23_PENALTY=0.657094
PRIMER_LEFT_23_SEQUENCE=TAATCCCCTCCATGAGATACA
PRIMER_RIGHT_23_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_23=581,21
PRIMER_RIGHT_23=1321,20
PRIMER_LEFT_23_TM=56.955
PRIMER_RIGHT_23_TM=60.657
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=5.6000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=741
PRIMER_PAIR_24_PENALTY=4.704588
PRIMER_LEFT_24_PENALTY=3.284668
PRIMER_RIGHT_24_PENALTY=1.419920
PRIMER_LEFT_24_SEQUENCE=AAATTTCGTTATGGCTGGAC
PRIMER_RIGHT_24_SEQUENCE=TTTTACTGGCGCAGAACACT
PRIMER_LEFT_24=643,20
PRIMER_RIGHT_24=1437,20
PRIMER_LEFT_24_TM=56.715
PRIMER_RIGHT_24_TM=58.580
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.1000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=795
PRIMER_PAIR_25_PENALTY=4.797183
PRIMER_LEFT_25_PENALTY=4.140089
PRIMER_RIGHT_25_PENALTY=0.657094
PRIMER_LEFT_25_SEQUENCE=ACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_25_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_25=579,23
PRIMER_RIGHT_25=1321,20
PRIMER_LEFT_25_TM=58.860
PRIMER_RIGHT_25_TM=60.657
PRIMER_LEFT_25_GC_PERCENT=43.478
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=5.6000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=743
PRIMER_PAIR_26_PENALTY=4.819574
PRIMER_LEFT_26_PENALTY=3.399655
PRIMER_RIGHT_26_PENALTY=1.419920
PRIMER_LEFT_26_SEQUENCE=GAGTTGACTAATCCCCTCCA
PRIMER_RIGHT_26_SEQUENCE=TTTTACTGGCGCAGAACACT
PRIMER_LEFT_26=573,20
PRIMER_RIGHT_26=1437,20
PRIMER_LEFT_26_TM=56.600
PRIMER_RIGHT_26_TM=58.580
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=865
PRIMER_PAIR_27_PENALTY=4.822029
PRIMER_LEFT_27_PENALTY=3.980488
PRIMER_RIGHT_27_PENALTY=0.841542
PRIMER_LEFT_27_SEQUENCE=CTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_27_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_27=580,22
PRIMER_RIGHT_27=1540,20
PRIMER_LEFT_27_TM=58.020
PRIMER_RIGHT_27_TM=59.158
PRIMER_LEFT_27_GC_PERCENT=45.455
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=5.6000
PRIMER_RIGHT_27_END_STABILITY=11.4000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=961
PRIMER_PAIR_28_PENALTY=4.836736
PRIMER_LEFT_28_PENALTY=4.559923
PRIMER_RIGHT_28_PENALTY=0.276812
PRIMER_LEFT_28_SEQUENCE=GACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_28_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_28=578,24
PRIMER_RIGHT_28=1327,20
PRIMER_LEFT_28_TM=60.560
PRIMER_RIGHT_28_TM=60.277
PRIMER_LEFT_28_GC_PERCENT=45.833
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=5.6000
PRIMER_RIGHT_28_END_STABILITY=9.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=750
PRIMER_PAIR_29_PENALTY=4.836736
PRIMER_LEFT_29_PENALTY=4.559923
PRIMER_RIGHT_29_PENALTY=0.276812
PRIMER_LEFT_29_SEQUENCE=TGACTAATCCCCTCCATGAGATAC
PRIMER_RIGHT_29_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_29=577,24
PRIMER_RIGHT_29=1327,20
PRIMER_LEFT_29_TM=60.560
PRIMER_RIGHT_29_TM=60.277
PRIMER_LEFT_29_GC_PERCENT=45.833
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=5.3000
PRIMER_RIGHT_29_END_STABILITY=9.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=751
PRIMER_PAIR_30_PENALTY=4.886468
PRIMER_LEFT_30_PENALTY=4.044927
PRIMER_RIGHT_30_PENALTY=0.841542
PRIMER_LEFT_30_SEQUENCE=TAATCCCCTCCATGAGATACA
PRIMER_RIGHT_30_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_30=581,21
PRIMER_RIGHT_30=1540,20
PRIMER_LEFT_30_TM=56.955
PRIMER_RIGHT_30_TM=59.158
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=5.6000
PRIMER_RIGHT_30_END_STABILITY=11.4000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=960
PRIMER_PAIR_31_PENALTY=4.981631
PRIMER_LEFT_31_PENALTY=4.140089
PRIMER_RIGHT_31_PENALTY=0.841542
PRIMER_LEFT_31_SEQUENCE=ACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_31_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_31=579,23
PRIMER_RIGHT_31=1540,20
PRIMER_LEFT_31_TM=58.860
PRIMER_RIGHT_31_TM=59.158
PRIMER_LEFT_31_GC_PERCENT=43.478
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=5.6000
PRIMER_RIGHT_31_END_STABILITY=11.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=962
PRIMER_PAIR_32_PENALTY=4.983300
PRIMER_LEFT_32_PENALTY=3.980488
PRIMER_RIGHT_32_PENALTY=1.002812
PRIMER_LEFT_32_SEQUENCE=CTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_32_SEQUENCE=TGCAGCCACAAGATTATCCC
PRIMER_LEFT_32=580,22
PRIMER_RIGHT_32=1303,20
PRIMER_LEFT_32_TM=58.020
PRIMER_RIGHT_32_TM=61.003
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=5.6000
PRIMER_RIGHT_32_END_STABILITY=9.3000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=724
PRIMER_PAIR_33_PENALTY=5.027492
PRIMER_LEFT_33_PENALTY=5.011180
PRIMER_RIGHT_33_PENALTY=0.016312
PRIMER_LEFT_33_SEQUENCE=GACTAATCCCCTCCATGAGATAC
PRIMER_RIGHT_33_SEQUENCE=GAATTTCATCCAAAAGCGGA
PRIMER_LEFT_33=578,23
PRIMER_RIGHT_33=1539,20
PRIMER_LEFT_33_TM=57.989
PRIMER_RIGHT_33_TM=60.016
PRIMER_LEFT_33_GC_PERCENT=47.826
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=5.3000
PRIMER_RIGHT_33_END_STABILITY=11.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=962
PRIMER_PAIR_34_PENALTY=5.047739
PRIMER_LEFT_34_PENALTY=4.044927
PRIMER_RIGHT_34_PENALTY=1.002812
PRIMER_LEFT_34_SEQUENCE=TAATCCCCTCCATGAGATACA
PRIMER_RIGHT_34_SEQUENCE=TGCAGCCACAAGATTATCCC
PRIMER_LEFT_34=581,21
PRIMER_RIGHT_34=1303,20
PRIMER_LEFT_34_TM=56.955
PRIMER_RIGHT_34_TM=61.003
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=5.6000
PRIMER_RIGHT_34_END_STABILITY=9.3000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=723
PRIMER_PAIR_35_PENALTY=5.142901
PRIMER_LEFT_35_PENALTY=4.140089
PRIMER_RIGHT_35_PENALTY=1.002812
PRIMER_LEFT_35_SEQUENCE=ACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_35_SEQUENCE=TGCAGCCACAAGATTATCCC
PRIMER_LEFT_35=579,23
PRIMER_RIGHT_35=1303,20
PRIMER_LEFT_35_TM=58.860
PRIMER_RIGHT_35_TM=61.003
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=5.6000
PRIMER_RIGHT_35_END_STABILITY=9.3000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=725
PRIMER_PAIR_36_PENALTY=5.217017
PRIMER_LEFT_36_PENALTY=4.559923
PRIMER_RIGHT_36_PENALTY=0.657094
PRIMER_LEFT_36_SEQUENCE=GACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_36_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_36=578,24
PRIMER_RIGHT_36=1321,20
PRIMER_LEFT_36_TM=60.560
PRIMER_RIGHT_36_TM=60.657
PRIMER_LEFT_36_GC_PERCENT=45.833
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=5.6000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=744
PRIMER_PAIR_37_PENALTY=5.217017
PRIMER_LEFT_37_PENALTY=4.559923
PRIMER_RIGHT_37_PENALTY=0.657094
PRIMER_LEFT_37_SEQUENCE=TGACTAATCCCCTCCATGAGATAC
PRIMER_RIGHT_37_SEQUENCE=GGCGAGTCTAACGGTTGATG
PRIMER_LEFT_37=577,24
PRIMER_RIGHT_37=1321,20
PRIMER_LEFT_37_TM=60.560
PRIMER_RIGHT_37_TM=60.657
PRIMER_LEFT_37_GC_PERCENT=45.833
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=5.3000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=745
PRIMER_PAIR_38_PENALTY=5.223685
PRIMER_LEFT_38_PENALTY=3.179598
PRIMER_RIGHT_38_PENALTY=2.044087
PRIMER_LEFT_38_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_38_SEQUENCE=GCCACAAGATTATCCCGTAAA
PRIMER_LEFT_38=582,20
PRIMER_RIGHT_38=1299,21
PRIMER_LEFT_38_TM=56.820
PRIMER_RIGHT_38_TM=58.956
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=5.6000
PRIMER_RIGHT_38_END_STABILITY=6.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=718
PRIMER_PAIR_39_PENALTY=5.287993
PRIMER_LEFT_39_PENALTY=5.011180
PRIMER_RIGHT_39_PENALTY=0.276812
PRIMER_LEFT_39_SEQUENCE=GACTAATCCCCTCCATGAGATAC
PRIMER_RIGHT_39_SEQUENCE=AGACATGGCGAGTCTAACGG
PRIMER_LEFT_39=578,23
PRIMER_RIGHT_39=1327,20
PRIMER_LEFT_39_TM=57.989
PRIMER_RIGHT_39_TM=60.277
PRIMER_LEFT_39_GC_PERCENT=47.826
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=5.3000
PRIMER_RIGHT_39_END_STABILITY=9.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=750
PRIMER_PAIR_40_PENALTY=5.315682
PRIMER_LEFT_40_PENALTY=3.179598
PRIMER_RIGHT_40_PENALTY=2.136083
PRIMER_LEFT_40_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_40_SEQUENCE=AGCCACAAGATTATCCCGTAAA
PRIMER_LEFT_40=582,20
PRIMER_RIGHT_40=1300,22
PRIMER_LEFT_40_TM=56.820
PRIMER_RIGHT_40_TM=59.864
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=5.6000
PRIMER_RIGHT_40_END_STABILITY=6.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=719
PRIMER_PAIR_41_PENALTY=5.323628
PRIMER_LEFT_41_PENALTY=3.179598
PRIMER_RIGHT_41_PENALTY=2.144030
PRIMER_LEFT_41_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_41_SEQUENCE=AAGAAACGGGTTTTCGCCTT
PRIMER_LEFT_41=582,20
PRIMER_RIGHT_41=1200,20
PRIMER_LEFT_41_TM=56.820
PRIMER_RIGHT_41_TM=62.144
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=5.6000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=619
PRIMER_PAIR_42_PENALTY=5.328755
PRIMER_LEFT_42_PENALTY=3.284668
PRIMER_RIGHT_42_PENALTY=2.044087
PRIMER_LEFT_42_SEQUENCE=AAATTTCGTTATGGCTGGAC
PRIMER_RIGHT_42_SEQUENCE=GCCACAAGATTATCCCGTAAA
PRIMER_LEFT_42=643,20
PRIMER_RIGHT_42=1299,21
PRIMER_LEFT_42_TM=56.715
PRIMER_RIGHT_42_TM=58.956
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.0000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=657
PRIMER_PAIR_43_PENALTY=5.397216
PRIMER_LEFT_43_PENALTY=3.179598
PRIMER_RIGHT_43_PENALTY=2.217618
PRIMER_LEFT_43_SEQUENCE=AATCCCCTCCATGAGATACA
PRIMER_RIGHT_43_SEQUENCE=ATGCAGCCACAAGATTATCCC
PRIMER_LEFT_43=582,20
PRIMER_RIGHT_43=1304,21
PRIMER_LEFT_43_TM=56.820
PRIMER_RIGHT_43_TM=61.218
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=5.6000
PRIMER_RIGHT_43_END_STABILITY=9.3000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=723
PRIMER_PAIR_44_PENALTY=5.400408
PRIMER_LEFT_44_PENALTY=3.980488
PRIMER_RIGHT_44_PENALTY=1.419920
PRIMER_LEFT_44_SEQUENCE=CTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_44_SEQUENCE=TTTTACTGGCGCAGAACACT
PRIMER_LEFT_44=580,22
PRIMER_RIGHT_44=1437,20
PRIMER_LEFT_44_TM=58.020
PRIMER_RIGHT_44_TM=58.580
PRIMER_LEFT_44_GC_PERCENT=45.455
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=5.6000
PRIMER_RIGHT_44_END_STABILITY=6.1000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=858
PRIMER_PAIR_45_PENALTY=5.401465
PRIMER_LEFT_45_PENALTY=4.559923
PRIMER_RIGHT_45_PENALTY=0.841542
PRIMER_LEFT_45_SEQUENCE=GACTAATCCCCTCCATGAGATACA
PRIMER_RIGHT_45_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_45=578,24
PRIMER_RIGHT_45=1540,20
PRIMER_LEFT_45_TM=60.560
PRIMER_RIGHT_45_TM=59.158
PRIMER_LEFT_45_GC_PERCENT=45.833
PRIMER_RIGHT_45_GC_PERCENT=40.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=5.6000
PRIMER_RIGHT_45_END_STABILITY=11.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=963
PRIMER_PAIR_46_PENALTY=5.401465
PRIMER_LEFT_46_PENALTY=4.559923
PRIMER_RIGHT_46_PENALTY=0.841542
PRIMER_LEFT_46_SEQUENCE=TGACTAATCCCCTCCATGAGATAC
PRIMER_RIGHT_46_SEQUENCE=AGAATTTCATCCAAAAGCGG
PRIMER_LEFT_46=577,24
PRIMER_RIGHT_46=1540,20
PRIMER_LEFT_46_TM=60.560
PRIMER_RIGHT_46_TM=59.158
PRIMER_LEFT_46_GC_PERCENT=45.833
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=5.3000
PRIMER_RIGHT_46_END_STABILITY=11.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=964
PRIMER_PAIR_47_PENALTY=5.420752
PRIMER_LEFT_47_PENALTY=3.284668
PRIMER_RIGHT_47_PENALTY=2.136083
PRIMER_LEFT_47_SEQUENCE=AAATTTCGTTATGGCTGGAC
PRIMER_RIGHT_47_SEQUENCE=AGCCACAAGATTATCCCGTAAA
PRIMER_LEFT_47=643,20
PRIMER_RIGHT_47=1300,22
PRIMER_LEFT_47_TM=56.715
PRIMER_RIGHT_47_TM=59.864
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=40.909
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.0000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=658
PRIMER_PAIR_48_PENALTY=5.428698
PRIMER_LEFT_48_PENALTY=3.284668
PRIMER_RIGHT_48_PENALTY=2.144030
PRIMER_LEFT_48_SEQUENCE=AAATTTCGTTATGGCTGGAC
PRIMER_RIGHT_48_SEQUENCE=AAGAAACGGGTTTTCGCCTT
PRIMER_LEFT_48=643,20
PRIMER_RIGHT_48=1200,20
PRIMER_LEFT_48_TM=56.715
PRIMER_RIGHT_48_TM=62.144
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=558
PRIMER_PAIR_49_PENALTY=5.443742
PRIMER_LEFT_49_PENALTY=3.399655
PRIMER_RIGHT_49_PENALTY=2.044087
PRIMER_LEFT_49_SEQUENCE=GAGTTGACTAATCCCCTCCA
PRIMER_RIGHT_49_SEQUENCE=GCCACAAGATTATCCCGTAAA
PRIMER_LEFT_49=573,20
PRIMER_RIGHT_49=1299,21
PRIMER_LEFT_49_TM=56.600
PRIMER_RIGHT_49_TM=58.956
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.0000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=727
=
SEQUENCE_ID=MAGIC.149_3_14387778_14388156_can-0_hi-0_7075_6161_TRUE
SEQUENCE_TARGET=950,478
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNAAGGCGACGGTGAGATGCGAACGAGTGTGAGAGTCGANNNNNNNNNNNNNNNNGGCGANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGAGNGTTTAGAGAGGAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_8513545_8513567_kn-0_bur-0_780411_178_TRUE
SEQUENCE_TARGET=950,122
SEQUENCE_TEMPLATE=TTTTTATTACTTTANTGTTTTTTATATGTCTATGGTGTGCTTATAGGTCTCTANATCTAGTTTTGTGTGTTATTTTCACGGCTATGATCTACAATTGGTTTTTATGTTCTTNCCTCTACCTGTGAGAGGAGAGTTAATATGTTATGGTNAAANGATATGATGCATAGCTGTGCAAAGANTAAAATTTAACTTCATATAAGGCTNCTTTTTGTGGCATATTGATGAAATATTAATNTTACACTTGGCTATTTTCATTGATCTGCTTAATGTTNCTGTTTATTGGTTTCATACGTGAATACAATCTAAACCAAATGATTGTGTAAATGTTTAGGTGGTGGACAACTACTACAGGCCGGACTTGAAGAAAGCTGCACTTGCTAGACTCAGCGCCATCAGCAAAGGTCTCAGGGTTGCCAAGTCNGGTGCCAAGCAAAGAAACAGACAAGCTTAAGCTTCTTCTTTCACAATCTGNTTTTTTTGAAGTAAAAGATTTTGTTGAACAGGTTTTATTTAAGTTGTTGCTTTGAAGTTGAACCATAANGCTATTCTCCTTTTTCNTTATCCTTATTATAAAGGAGTTAGAAGTTTATCTTNTTANNNNNNNNNNNNNNNNNNTGCTTGTGTTACATTTTGTTANATCNTTTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAACNAAGTCCTTATATNTNATGANNTTTGNAAGCCTANANNNNNNNNNNNNNNNNNNNNNNNNNNTTTANNTTNNTTGCATCANATGTGATGTTCTTGTGGACTNTAATGTTGACNTTNTCNTTNGATCCCATCAAAAACNAACTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACNGAAAGGTTTATATTAGGGTTTCNTAGACNTGNNTTAATTNTGTNCTCTNGCTGTNGCCGTCTCCAACNCCATTTCGAAGNNNNGNGAGANCGANGCNNNANNGAGNAAGNAAANNGNAGAAAGGAANNGGNNGNNANNANGGAGNNCNNNANCANNNNNNACANNNNNCTCCANAGNNNGTANNNNNTAAANCNTNAANNNTNNNNANCTTATNTNCANTNTCTCGTTTCCGNTTTNNTTAAGTTCGTTCTNGGNNTAGNNNNTAANNATTAGACTNAAATTGCAACTTNTNTTGATTNNAATTAGGTTTCTTGATGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNANTCTNTAGATGAGTAANGATCATAAANATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTNGNNNCCTTTTATTGNATAGAAAGGTTGGCTTGTTGCTTAGGTNCATTTTNNATTNGAGNTTTTTTCGTTGTCANNATTGATCNNGTGGTNNNNNNNNNNNNNNNNNNNNTNNTTTCTAAATTGAACAGCACCTTCAAGAAGAAGGCACCCAATGCCATTAAAGAGATCAGGAAGTTTGCATTGAAAGCAATGGGAACNAANGACGTTAGAGTNGATGTTAAACTCAACAAGCAGATATGGAGCAAAGGTATCCGAGGTCCCCCGNGGAGNATNAGAGTCCGTGTTGCCCGTAAGAGAAACGATGATGAAGATGCCAAGGAAGAGTTCTTCTCNCTCGTCACNGTCGCTGAGATTCCGGCTGAAGGATTATCTGGTTTGGGTACTAAGGTCATTGAAGAAGAGGAGTGAAAATTGGTGAATGTACACTCACACANCCTTATCTTTNGATGNAAAGACGAAANNAAAAGACTTTGCTTGTTTTGATCACTTACCTATTTTGGTCATTTATCATNTCTTATGTTTTAAGTTCTATAGACAACAGATTTGGTACTACTCATACGAGTTTGATCTTTTTGTGACGCANTTTACTTCGCAATCATCTTTCTGATTCTGTACCAGACCGGTTTAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_5_14855291_14855587_edi-0_ct-1_95063_84665_TRUE
SEQUENCE_TARGET=950,396
SEQUENCE_TEMPLATE=CGCCTATGCTCATCCCCGACTTTTTCGGGAATATGATAGTAACTNCATATACAGGAGTAACATAAATAAATACTCTTTTGATGCACAAAGATGAGTAGAAGATATGTNTATTACCGTCTTTGTAAGCACCAGTGTTGATACTAATAATCTCATAAGGGCCAAGAGAATCATCAGTATTCAGAGTGAAAGTTGCAAAGAAGTCCACAATACGTTGAACCTCTGTGCTGTTGAATTTACTGTTTAATTCACTGTACTCAGGGAAAATCTTCATGTTCATGAATAGCCTTGGACCCGACTGCCATGCAAATGTATCAATAGATATCTGATACGGGTTTATCCCGAGATTTTTCGGGGATGCTACATCGAGCATGTAGGAGACTTTGTAAGGTCGAAAGTCCGAGAAACTTGGACTCCTAAGCCGAAGATCAATGCCAAGTGGTGCAGCGCAGAAACATGCAACTGGAGACCCAATCACGTAGNCATAGTTTTCGCTTACGGGGCACGACTGACGTTTGCAGTCTCCCGTCGAGATGGTTTCCGAAGAAGTTGCNGGAGATTCCACTTCTAACGTTGAGATACCACAGAGATCAGCTAACTTTCCCGCATTAACATTTGCACAAACCGGGTTTCCGTAAAGCCTGCTCATCAAAACAGAGTTCNCTCATTTCTCTATCCAACATTCCAAATTATTAATCTTTTGTTCTTTTTCAAAACATANTCTTACTTGACTGTGACATTGGAGGGAGGGTTTAGAAGAACACTTGACACATTTGAAAACATGTTGTTCCTCAAGTCCCTGCAAGTTGTTNCAACCAAAANTCAATNAACAATTACTAAGNTTTCTCTAGTGTTTTGTAGATATGACGNGTCTTACAAGATAAGNTTTTCCTCTGCCTTCAAGATCCTATTTTCCCATATCACAGGAATCTCCCCACTNAANTTGTTGTTCTGCACTTGCCTGAAAATTTAAGACAAANNNNNNNNNNNTNTNNTTTNTTTNNCNAAANAAAAGAAGAGCAAAAGAGAGTNTAATTACAGTCTCTGTAACCGTGGAAGGCCTGAAAAATTNGAAGGAATGGATCCGNTGAGCAAATTGTTGTACAAGTTTCTGNATACAATAAACAAATGCAGACATGTTTGTCAACAAAAATGTAACNACANATCAATTGTATTCNATTTCTTGGCCATGNCACTTACATAGTTGTGATGTTTGCAGNGAACTTGTTCTTGGGAATTTCTCCTGTGAGTTTGTTGGANGAAATATCTCTGAAGCATGAAGAGNAAAAAAGCCTNAGANACTAATGTAACATGGATTGTTAAGNNNNTTTTCTTGTTANAGTGACTCACAAGTAATAGAGAACCNGTGATTTGCTCAAATCAGGAATTGGTCCTTCTAGNTTGCAGTTTCTGAGACTCCTGTTAATGCGTGAGACANAAAATCAGTACGGCACAGGGAAAACATAAGATTTTACTATGTTCAGTTTCTTACAATTTGACGAGATTTGGTATCGATCCGTAAGAAGATGGGATCTCTGTGCCATCAAAGTTGCTACCGTCCAATTGTCTGTAAATNATGAAGCAAACTAATTTGAATGTGGAAGAAAGTTAGTCGTCTTCGATAAGAAGTTGAAGACACTTACAGAATTCTCAAGCTTGGCATCTGAGCGAGCTCCGGTGGAAGATTACCAGTTAGCTTGTTATTGTCCATCAAGCTTCACAAGTTGAGCCTTGATGATCAGATTNGAATAAGATAAACAGANTTAAAGGTAACCAAGAAACTTACAAGTGCAGAACATTGGTTAANGTAGAGTACTCGGGCGGAATCTGACCCGTGATTGAGTTATTGTTCATGTGACTAGCAAAGAANGTAAAAGTTATGTCAGANGGGTTTGNAAGGAAGAGANAAGANANNTANGAGAGAAGGAGATCTCACAAATGCTTCAGTTTTTTCAAATTTGCCAAGGACGTTGGAAGTTTTCCGNTAATTTCATTGTANTCTATCTGAAGGATNAAGAGATTGGAAAGAGAACCGAGTTCTTGAGGCAAAGATCCTGTCAGTTGATTTCCACTTAATAGCCTGACAAAANANAACATNAATCTGTTAAGTTATGCATCCATGAAGAGGAAATATTGATGAAACAATGATCAAGACTACTTACAAGAAGATGAGATGAGTNAAGTTTCCTAGTTCTGGTGGAATCTGACCGGTGAGATCGTTCCACATGAAGTTCCTGTGATCAGAAAATATAAAAAGNAGAGACGTTGANATNTATAGTTTGATTTNAAAAAACCANAN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.045389
PRIMER_LEFT_0_PENALTY=0.038444
PRIMER_RIGHT_0_PENALTY=0.006945
PRIMER_LEFT_0_SEQUENCE=ACTGTGACATTGGAGGGAGG
PRIMER_RIGHT_0_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_0=727,20
PRIMER_RIGHT_0=1454,20
PRIMER_LEFT_0_TM=59.962
PRIMER_RIGHT_0_TM=59.993
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=728
PRIMER_PAIR_1_PENALTY=0.094921
PRIMER_LEFT_1_PENALTY=0.087977
PRIMER_RIGHT_1_PENALTY=0.006945
PRIMER_LEFT_1_SEQUENCE=TGACTGTGACATTGGAGGGA
PRIMER_RIGHT_1_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_1=725,20
PRIMER_RIGHT_1=1454,20
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=730
PRIMER_PAIR_2_PENALTY=0.161823
PRIMER_LEFT_2_PENALTY=0.154878
PRIMER_RIGHT_2_PENALTY=0.006945
PRIMER_LEFT_2_SEQUENCE=TCAGCTAACTTTCCCGCATT
PRIMER_RIGHT_2_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_2=587,20
PRIMER_RIGHT_2=1454,20
PRIMER_LEFT_2_TM=59.845
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=868
PRIMER_PAIR_3_PENALTY=0.199862
PRIMER_LEFT_3_PENALTY=0.192918
PRIMER_RIGHT_3_PENALTY=0.006945
PRIMER_LEFT_3_SEQUENCE=CGAGATGGTTTCCGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_3=526,20
PRIMER_RIGHT_3=1454,20
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=59.993
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=929
PRIMER_PAIR_4_PENALTY=0.313613
PRIMER_LEFT_4_PENALTY=0.306669
PRIMER_RIGHT_4_PENALTY=0.006945
PRIMER_LEFT_4_SEQUENCE=ACTGACGTTTGCAGTCTCCC
PRIMER_RIGHT_4_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_4=504,20
PRIMER_RIGHT_4=1454,20
PRIMER_LEFT_4_TM=60.307
PRIMER_RIGHT_4_TM=59.993
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=951
PRIMER_PAIR_5_PENALTY=0.322818
PRIMER_LEFT_5_PENALTY=0.038444
PRIMER_RIGHT_5_PENALTY=0.284374
PRIMER_LEFT_5_SEQUENCE=ACTGTGACATTGGAGGGAGG
PRIMER_RIGHT_5_SEQUENCE=CAGACAATTGGACGGTAGCA
PRIMER_LEFT_5=727,20
PRIMER_RIGHT_5=1566,20
PRIMER_LEFT_5_TM=59.962
PRIMER_RIGHT_5_TM=59.716
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=7.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=840
PRIMER_PAIR_6_PENALTY=0.359198
PRIMER_LEFT_6_PENALTY=0.352253
PRIMER_RIGHT_6_PENALTY=0.006945
PRIMER_LEFT_6_SEQUENCE=TTCCGTAAAGCCTGCTCATC
PRIMER_RIGHT_6_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_6=627,20
PRIMER_RIGHT_6=1454,20
PRIMER_LEFT_6_TM=60.352
PRIMER_RIGHT_6_TM=59.993
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=828
PRIMER_PAIR_7_PENALTY=0.359198
PRIMER_LEFT_7_PENALTY=0.352253
PRIMER_RIGHT_7_PENALTY=0.006945
PRIMER_LEFT_7_SEQUENCE=GATCAGCTAACTTTCCCGCA
PRIMER_RIGHT_7_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_7=585,20
PRIMER_RIGHT_7=1454,20
PRIMER_LEFT_7_TM=60.352
PRIMER_RIGHT_7_TM=59.993
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=11.7000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=870
PRIMER_PAIR_8_PENALTY=0.372351
PRIMER_LEFT_8_PENALTY=0.087977
PRIMER_RIGHT_8_PENALTY=0.284374
PRIMER_LEFT_8_SEQUENCE=TGACTGTGACATTGGAGGGA
PRIMER_RIGHT_8_SEQUENCE=CAGACAATTGGACGGTAGCA
PRIMER_LEFT_8=725,20
PRIMER_RIGHT_8=1566,20
PRIMER_LEFT_8_TM=60.088
PRIMER_RIGHT_8_TM=59.716
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=7.5000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=842
PRIMER_PAIR_9_PENALTY=0.403584
PRIMER_LEFT_9_PENALTY=0.396640
PRIMER_RIGHT_9_PENALTY=0.006945
PRIMER_LEFT_9_SEQUENCE=CATAGTTTTCGCTTACGGGG
PRIMER_RIGHT_9_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_9=480,20
PRIMER_RIGHT_9=1454,20
PRIMER_LEFT_9_TM=59.603
PRIMER_RIGHT_9_TM=59.993
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=12.9000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=975
PRIMER_PAIR_10_PENALTY=0.403615
PRIMER_LEFT_10_PENALTY=0.038444
PRIMER_RIGHT_10_PENALTY=0.365170
PRIMER_LEFT_10_SEQUENCE=ACTGTGACATTGGAGGGAGG
PRIMER_RIGHT_10_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_10=727,20
PRIMER_RIGHT_10=1463,20
PRIMER_LEFT_10_TM=59.962
PRIMER_RIGHT_10_TM=60.365
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=11.1000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=737
PRIMER_PAIR_11_PENALTY=0.439253
PRIMER_LEFT_11_PENALTY=0.154878
PRIMER_RIGHT_11_PENALTY=0.284374
PRIMER_LEFT_11_SEQUENCE=TCAGCTAACTTTCCCGCATT
PRIMER_RIGHT_11_SEQUENCE=CAGACAATTGGACGGTAGCA
PRIMER_LEFT_11=587,20
PRIMER_RIGHT_11=1566,20
PRIMER_LEFT_11_TM=59.845
PRIMER_RIGHT_11_TM=59.716
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=7.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=980
PRIMER_PAIR_12_PENALTY=0.453147
PRIMER_LEFT_12_PENALTY=0.087977
PRIMER_RIGHT_12_PENALTY=0.365170
PRIMER_LEFT_12_SEQUENCE=TGACTGTGACATTGGAGGGA
PRIMER_RIGHT_12_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_12=725,20
PRIMER_RIGHT_12=1463,20
PRIMER_LEFT_12_TM=60.088
PRIMER_RIGHT_12_TM=60.365
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=11.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=739
PRIMER_PAIR_13_PENALTY=0.487427
PRIMER_LEFT_13_PENALTY=0.480482
PRIMER_RIGHT_13_PENALTY=0.006945
PRIMER_LEFT_13_SEQUENCE=TTGACTGTGACATTGGAGGG
PRIMER_RIGHT_13_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_13=724,20
PRIMER_RIGHT_13=1454,20
PRIMER_LEFT_13_TM=59.520
PRIMER_RIGHT_13_TM=59.993
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=731
PRIMER_PAIR_14_PENALTY=0.520049
PRIMER_LEFT_14_PENALTY=0.154878
PRIMER_RIGHT_14_PENALTY=0.365170
PRIMER_LEFT_14_SEQUENCE=TCAGCTAACTTTCCCGCATT
PRIMER_RIGHT_14_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_14=587,20
PRIMER_RIGHT_14=1463,20
PRIMER_LEFT_14_TM=59.845
PRIMER_RIGHT_14_TM=60.365
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=11.1000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=877
PRIMER_PAIR_15_PENALTY=0.526857
PRIMER_LEFT_15_PENALTY=0.519913
PRIMER_RIGHT_15_PENALTY=0.006945
PRIMER_LEFT_15_SEQUENCE=GCAACTGGAGACCCAATCAC
PRIMER_RIGHT_15_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_15=455,20
PRIMER_RIGHT_15=1454,20
PRIMER_LEFT_15_TM=60.520
PRIMER_RIGHT_15_TM=59.993
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.3000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=1000
PRIMER_PAIR_16_PENALTY=0.555127
PRIMER_LEFT_16_PENALTY=0.038444
PRIMER_RIGHT_16_PENALTY=0.516683
PRIMER_LEFT_16_SEQUENCE=ACTGTGACATTGGAGGGAGG
PRIMER_RIGHT_16_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_16=727,20
PRIMER_RIGHT_16=1455,20
PRIMER_LEFT_16_TM=59.962
PRIMER_RIGHT_16_TM=60.517
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=729
PRIMER_PAIR_17_PENALTY=0.555127
PRIMER_LEFT_17_PENALTY=0.038444
PRIMER_RIGHT_17_PENALTY=0.516683
PRIMER_LEFT_17_SEQUENCE=ACTGTGACATTGGAGGGAGG
PRIMER_RIGHT_17_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_17=727,20
PRIMER_RIGHT_17=1456,20
PRIMER_LEFT_17_TM=59.962
PRIMER_RIGHT_17_TM=60.517
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=730
PRIMER_PAIR_18_PENALTY=0.558088
PRIMER_LEFT_18_PENALTY=0.192918
PRIMER_RIGHT_18_PENALTY=0.365170
PRIMER_LEFT_18_SEQUENCE=CGAGATGGTTTCCGAAGAAG
PRIMER_RIGHT_18_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_18=526,20
PRIMER_RIGHT_18=1463,20
PRIMER_LEFT_18_TM=59.807
PRIMER_RIGHT_18_TM=60.365
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=11.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=938
PRIMER_PAIR_19_PENALTY=0.604660
PRIMER_LEFT_19_PENALTY=0.087977
PRIMER_RIGHT_19_PENALTY=0.516683
PRIMER_LEFT_19_SEQUENCE=TGACTGTGACATTGGAGGGA
PRIMER_RIGHT_19_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_19=725,20
PRIMER_RIGHT_19=1455,20
PRIMER_LEFT_19_TM=60.088
PRIMER_RIGHT_19_TM=60.517
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=731
PRIMER_PAIR_20_PENALTY=0.604660
PRIMER_LEFT_20_PENALTY=0.087977
PRIMER_RIGHT_20_PENALTY=0.516683
PRIMER_LEFT_20_SEQUENCE=TGACTGTGACATTGGAGGGA
PRIMER_RIGHT_20_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_20=725,20
PRIMER_RIGHT_20=1456,20
PRIMER_LEFT_20_TM=60.088
PRIMER_RIGHT_20_TM=60.517
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=732
PRIMER_PAIR_21_PENALTY=0.636627
PRIMER_LEFT_21_PENALTY=0.352253
PRIMER_RIGHT_21_PENALTY=0.284374
PRIMER_LEFT_21_SEQUENCE=TTCCGTAAAGCCTGCTCATC
PRIMER_RIGHT_21_SEQUENCE=CAGACAATTGGACGGTAGCA
PRIMER_LEFT_21=627,20
PRIMER_RIGHT_21=1566,20
PRIMER_LEFT_21_TM=60.352
PRIMER_RIGHT_21_TM=59.716
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.6000
PRIMER_RIGHT_21_END_STABILITY=7.5000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=940
PRIMER_PAIR_22_PENALTY=0.636627
PRIMER_LEFT_22_PENALTY=0.352253
PRIMER_RIGHT_22_PENALTY=0.284374
PRIMER_LEFT_22_SEQUENCE=GATCAGCTAACTTTCCCGCA
PRIMER_RIGHT_22_SEQUENCE=CAGACAATTGGACGGTAGCA
PRIMER_LEFT_22=585,20
PRIMER_RIGHT_22=1566,20
PRIMER_LEFT_22_TM=60.352
PRIMER_RIGHT_22_TM=59.716
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=11.7000
PRIMER_RIGHT_22_END_STABILITY=7.5000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=982
PRIMER_PAIR_23_PENALTY=0.671562
PRIMER_LEFT_23_PENALTY=0.154878
PRIMER_RIGHT_23_PENALTY=0.516683
PRIMER_LEFT_23_SEQUENCE=TCAGCTAACTTTCCCGCATT
PRIMER_RIGHT_23_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_23=587,20
PRIMER_RIGHT_23=1455,20
PRIMER_LEFT_23_TM=59.845
PRIMER_RIGHT_23_TM=60.517
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.4000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=869
PRIMER_PAIR_24_PENALTY=0.671562
PRIMER_LEFT_24_PENALTY=0.154878
PRIMER_RIGHT_24_PENALTY=0.516683
PRIMER_LEFT_24_SEQUENCE=TCAGCTAACTTTCCCGCATT
PRIMER_RIGHT_24_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_24=587,20
PRIMER_RIGHT_24=1456,20
PRIMER_LEFT_24_TM=59.845
PRIMER_RIGHT_24_TM=60.517
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.4000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=870
PRIMER_PAIR_25_PENALTY=0.671839
PRIMER_LEFT_25_PENALTY=0.306669
PRIMER_RIGHT_25_PENALTY=0.365170
PRIMER_LEFT_25_SEQUENCE=ACTGACGTTTGCAGTCTCCC
PRIMER_RIGHT_25_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_25=504,20
PRIMER_RIGHT_25=1463,20
PRIMER_LEFT_25_TM=60.307
PRIMER_RIGHT_25_TM=60.365
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=11.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=960
PRIMER_PAIR_26_PENALTY=0.709601
PRIMER_LEFT_26_PENALTY=0.192918
PRIMER_RIGHT_26_PENALTY=0.516683
PRIMER_LEFT_26_SEQUENCE=CGAGATGGTTTCCGAAGAAG
PRIMER_RIGHT_26_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_26=526,20
PRIMER_RIGHT_26=1455,20
PRIMER_LEFT_26_TM=59.807
PRIMER_RIGHT_26_TM=60.517
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=930
PRIMER_PAIR_27_PENALTY=0.709601
PRIMER_LEFT_27_PENALTY=0.192918
PRIMER_RIGHT_27_PENALTY=0.516683
PRIMER_LEFT_27_SEQUENCE=CGAGATGGTTTCCGAAGAAG
PRIMER_RIGHT_27_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_27=526,20
PRIMER_RIGHT_27=1456,20
PRIMER_LEFT_27_TM=59.807
PRIMER_RIGHT_27_TM=60.517
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=931
PRIMER_PAIR_28_PENALTY=0.717424
PRIMER_LEFT_28_PENALTY=0.352253
PRIMER_RIGHT_28_PENALTY=0.365170
PRIMER_LEFT_28_SEQUENCE=TTCCGTAAAGCCTGCTCATC
PRIMER_RIGHT_28_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_28=627,20
PRIMER_RIGHT_28=1463,20
PRIMER_LEFT_28_TM=60.352
PRIMER_RIGHT_28_TM=60.365
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.6000
PRIMER_RIGHT_28_END_STABILITY=11.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=837
PRIMER_PAIR_29_PENALTY=0.717424
PRIMER_LEFT_29_PENALTY=0.352253
PRIMER_RIGHT_29_PENALTY=0.365170
PRIMER_LEFT_29_SEQUENCE=GATCAGCTAACTTTCCCGCA
PRIMER_RIGHT_29_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_29=585,20
PRIMER_RIGHT_29=1463,20
PRIMER_LEFT_29_TM=60.352
PRIMER_RIGHT_29_TM=60.365
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=11.7000
PRIMER_RIGHT_29_END_STABILITY=11.1000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=879
PRIMER_PAIR_30_PENALTY=0.761810
PRIMER_LEFT_30_PENALTY=0.396640
PRIMER_RIGHT_30_PENALTY=0.365170
PRIMER_LEFT_30_SEQUENCE=CATAGTTTTCGCTTACGGGG
PRIMER_RIGHT_30_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_30=480,20
PRIMER_RIGHT_30=1463,20
PRIMER_LEFT_30_TM=59.603
PRIMER_RIGHT_30_TM=60.365
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=12.9000
PRIMER_RIGHT_30_END_STABILITY=11.1000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=984
PRIMER_PAIR_31_PENALTY=0.764856
PRIMER_LEFT_31_PENALTY=0.480482
PRIMER_RIGHT_31_PENALTY=0.284374
PRIMER_LEFT_31_SEQUENCE=TTGACTGTGACATTGGAGGG
PRIMER_RIGHT_31_SEQUENCE=CAGACAATTGGACGGTAGCA
PRIMER_LEFT_31=724,20
PRIMER_RIGHT_31=1566,20
PRIMER_LEFT_31_TM=59.520
PRIMER_RIGHT_31_TM=59.716
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=7.5000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=843
PRIMER_PAIR_32_PENALTY=0.823352
PRIMER_LEFT_32_PENALTY=0.306669
PRIMER_RIGHT_32_PENALTY=0.516683
PRIMER_LEFT_32_SEQUENCE=ACTGACGTTTGCAGTCTCCC
PRIMER_RIGHT_32_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_32=504,20
PRIMER_RIGHT_32=1455,20
PRIMER_LEFT_32_TM=60.307
PRIMER_RIGHT_32_TM=60.517
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=952
PRIMER_PAIR_33_PENALTY=0.823352
PRIMER_LEFT_33_PENALTY=0.306669
PRIMER_RIGHT_33_PENALTY=0.516683
PRIMER_LEFT_33_SEQUENCE=ACTGACGTTTGCAGTCTCCC
PRIMER_RIGHT_33_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_33=504,20
PRIMER_RIGHT_33=1456,20
PRIMER_LEFT_33_TM=60.307
PRIMER_RIGHT_33_TM=60.517
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=9.4000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=953
PRIMER_PAIR_34_PENALTY=0.845653
PRIMER_LEFT_34_PENALTY=0.480482
PRIMER_RIGHT_34_PENALTY=0.365170
PRIMER_LEFT_34_SEQUENCE=TTGACTGTGACATTGGAGGG
PRIMER_RIGHT_34_SEQUENCE=TTATGTTTTCCCTGTGCCGT
PRIMER_LEFT_34=724,20
PRIMER_RIGHT_34=1463,20
PRIMER_LEFT_34_TM=59.520
PRIMER_RIGHT_34_TM=60.365
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.4000
PRIMER_RIGHT_34_END_STABILITY=11.1000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=740
PRIMER_PAIR_35_PENALTY=0.868936
PRIMER_LEFT_35_PENALTY=0.352253
PRIMER_RIGHT_35_PENALTY=0.516683
PRIMER_LEFT_35_SEQUENCE=TTCCGTAAAGCCTGCTCATC
PRIMER_RIGHT_35_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_35=627,20
PRIMER_RIGHT_35=1455,20
PRIMER_LEFT_35_TM=60.352
PRIMER_RIGHT_35_TM=60.517
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=6.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=829
PRIMER_PAIR_36_PENALTY=0.868936
PRIMER_LEFT_36_PENALTY=0.352253
PRIMER_RIGHT_36_PENALTY=0.516683
PRIMER_LEFT_36_SEQUENCE=TTCCGTAAAGCCTGCTCATC
PRIMER_RIGHT_36_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_36=627,20
PRIMER_RIGHT_36=1456,20
PRIMER_LEFT_36_TM=60.352
PRIMER_RIGHT_36_TM=60.517
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.6000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=830
PRIMER_PAIR_37_PENALTY=0.868936
PRIMER_LEFT_37_PENALTY=0.352253
PRIMER_RIGHT_37_PENALTY=0.516683
PRIMER_LEFT_37_SEQUENCE=GATCAGCTAACTTTCCCGCA
PRIMER_RIGHT_37_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_37=585,20
PRIMER_RIGHT_37=1455,20
PRIMER_LEFT_37_TM=60.352
PRIMER_RIGHT_37_TM=60.517
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=11.7000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=871
PRIMER_PAIR_38_PENALTY=0.868936
PRIMER_LEFT_38_PENALTY=0.352253
PRIMER_RIGHT_38_PENALTY=0.516683
PRIMER_LEFT_38_SEQUENCE=GATCAGCTAACTTTCCCGCA
PRIMER_RIGHT_38_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_38=585,20
PRIMER_RIGHT_38=1456,20
PRIMER_LEFT_38_TM=60.352
PRIMER_RIGHT_38_TM=60.517
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=11.7000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=872
PRIMER_PAIR_39_PENALTY=0.913323
PRIMER_LEFT_39_PENALTY=0.396640
PRIMER_RIGHT_39_PENALTY=0.516683
PRIMER_LEFT_39_SEQUENCE=CATAGTTTTCGCTTACGGGG
PRIMER_RIGHT_39_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_39=480,20
PRIMER_RIGHT_39=1455,20
PRIMER_LEFT_39_TM=59.603
PRIMER_RIGHT_39_TM=60.517
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=12.9000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=976
PRIMER_PAIR_40_PENALTY=0.913323
PRIMER_LEFT_40_PENALTY=0.396640
PRIMER_RIGHT_40_PENALTY=0.516683
PRIMER_LEFT_40_SEQUENCE=CATAGTTTTCGCTTACGGGG
PRIMER_RIGHT_40_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_40=480,20
PRIMER_RIGHT_40=1456,20
PRIMER_LEFT_40_TM=59.603
PRIMER_RIGHT_40_TM=60.517
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=12.9000
PRIMER_RIGHT_40_END_STABILITY=6.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=977
PRIMER_PAIR_41_PENALTY=0.944704
PRIMER_LEFT_41_PENALTY=0.937760
PRIMER_RIGHT_41_PENALTY=0.006945
PRIMER_LEFT_41_SEQUENCE=CGTAAAGCCTGCTCATCAAA
PRIMER_RIGHT_41_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_41=630,20
PRIMER_RIGHT_41=1454,20
PRIMER_LEFT_41_TM=59.062
PRIMER_RIGHT_41_TM=59.993
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=7.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=825
PRIMER_PAIR_42_PENALTY=0.997165
PRIMER_LEFT_42_PENALTY=0.480482
PRIMER_RIGHT_42_PENALTY=0.516683
PRIMER_LEFT_42_SEQUENCE=TTGACTGTGACATTGGAGGG
PRIMER_RIGHT_42_SEQUENCE=TCCCTGTGCCGTACTGATTT
PRIMER_LEFT_42=724,20
PRIMER_RIGHT_42=1455,20
PRIMER_LEFT_42_TM=59.520
PRIMER_RIGHT_42_TM=60.517
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.4000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=732
PRIMER_PAIR_43_PENALTY=0.997165
PRIMER_LEFT_43_PENALTY=0.480482
PRIMER_RIGHT_43_PENALTY=0.516683
PRIMER_LEFT_43_SEQUENCE=TTGACTGTGACATTGGAGGG
PRIMER_RIGHT_43_SEQUENCE=TTCCCTGTGCCGTACTGATT
PRIMER_LEFT_43=724,20
PRIMER_RIGHT_43=1456,20
PRIMER_LEFT_43_TM=59.520
PRIMER_RIGHT_43_TM=60.517
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=733
PRIMER_PAIR_44_PENALTY=1.059290
PRIMER_LEFT_44_PENALTY=1.052345
PRIMER_RIGHT_44_PENALTY=0.006945
PRIMER_LEFT_44_SEQUENCE=AGTCTCCCGTCGAGATGGTT
PRIMER_RIGHT_44_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_44=516,20
PRIMER_RIGHT_44=1454,20
PRIMER_LEFT_44_TM=61.052
PRIMER_RIGHT_44_TM=59.993
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=939
PRIMER_PAIR_45_PENALTY=1.060459
PRIMER_LEFT_45_PENALTY=1.053515
PRIMER_RIGHT_45_PENALTY=0.006945
PRIMER_LEFT_45_SEQUENCE=TGGAGGGAGGGTTTAGAAGAA
PRIMER_RIGHT_45_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_45=737,21
PRIMER_RIGHT_45=1454,20
PRIMER_LEFT_45_TM=60.054
PRIMER_RIGHT_45_TM=59.993
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=718
PRIMER_PAIR_46_PENALTY=1.060459
PRIMER_LEFT_46_PENALTY=1.053515
PRIMER_RIGHT_46_PENALTY=0.006945
PRIMER_LEFT_46_SEQUENCE=TTGGAGGGAGGGTTTAGAAGA
PRIMER_RIGHT_46_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_46=736,21
PRIMER_RIGHT_46=1454,20
PRIMER_LEFT_46_TM=60.054
PRIMER_RIGHT_46_TM=59.993
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=7.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=719
PRIMER_PAIR_47_PENALTY=1.115987
PRIMER_LEFT_47_PENALTY=1.109043
PRIMER_RIGHT_47_PENALTY=0.006945
PRIMER_LEFT_47_SEQUENCE=ATCAGCTAACTTTCCCGCATT
PRIMER_RIGHT_47_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_47=586,21
PRIMER_RIGHT_47=1454,20
PRIMER_LEFT_47_TM=60.109
PRIMER_RIGHT_47_TM=59.993
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.4000
PRIMER_RIGHT_47_END_STABILITY=7.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=869
PRIMER_PAIR_48_PENALTY=1.158969
PRIMER_LEFT_48_PENALTY=1.152025
PRIMER_RIGHT_48_PENALTY=0.006945
PRIMER_LEFT_48_SEQUENCE=GTGACATTGGAGGGAGGGTT
PRIMER_RIGHT_48_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_48=730,20
PRIMER_RIGHT_48=1454,20
PRIMER_LEFT_48_TM=61.152
PRIMER_RIGHT_48_TM=59.993
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=7.2000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=725
PRIMER_PAIR_49_PENALTY=1.194271
PRIMER_LEFT_49_PENALTY=1.187326
PRIMER_RIGHT_49_PENALTY=0.006945
PRIMER_LEFT_49_SEQUENCE=GACATTGGAGGGAGGGTTTAG
PRIMER_RIGHT_49_SEQUENCE=CCCTGTGCCGTACTGATTTT
PRIMER_LEFT_49=732,21
PRIMER_RIGHT_49=1454,20
PRIMER_LEFT_49_TM=59.813
PRIMER_RIGHT_49_TM=59.993
PRIMER_LEFT_49_GC_PERCENT=52.381
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.3000
PRIMER_RIGHT_49_END_STABILITY=7.2000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=723
=
SEQUENCE_ID=MAGIC.433_1_24586132_24586188_ler-0_can-0_410377_454810_TRUE
SEQUENCE_TARGET=950,156
SEQUENCE_TEMPLATE=CTGCAGAAAGTGAGAAAGATATAGGAACTTTGCACAAAGAAATGCTAACCTTACAACTCGGACAGGATAGCCCATTTTGCAACTAAGTCTTAGAGCTTCATTCATGTTTTTAAACGCCTGGTCAGAAGACTGTATCTTGTTAACTCTTTTGTTTCCACTGAGATCCCTTCCACCACTGCAACACACATTCACACNNATTAAGATAAATTGGAAAAAGGCTACTTCAATAAGAAGGGAAACAGGTGACAAATATAATGTAAAAAATCAAACCTTCCTGTGTAGAGAAACCATTCACCATGATCCTCATCATCGTCATAACCTCCAGAGAGGGCCACAGACTGAGCTCCAACCGCTGCTTGCCCGGCAATGCCAGCAACATGCGGGAAATGAACTCCCCACTGCCTACACTCTTGCCTGTCCTCCCAAGATTCTCCAACCAAAACACCTTGGTTTCTAGTGACATCATTCGCAGCTGGTATGGGACCAAAATGATCACGAGGTATTGTCACAAAAAACTTACCGCTAGCAGCATTAGCTTTCCCAGTTTTTACTGCCCGCTCGGTAGTAAATGCTTTGTCAGGACGGTCTTGGTTGCGGATAATATGATGAACCTTGGCCGCAGTTGCCTCACCAGAACATTTGGTAACATTTGCTAAACGAATAGCAGAAACTAGAGCTAAGTTGATGCGAGGATTTTTCGCCACATGTCTCGGAATTTTGCTTCGGCATATCATACAAGTTAGCTTCCCTTGACCTACTGCCCATTTCTCGAAACATTTCAAACAGAAATTGTGCCCACACGGTGTCTACAAAACACAAAAGAACAATACATCCATTAGTCTGNNNNNNNNNNNNNCTAATCATCATTATCAAGTATACAAATAAACTAGCGATTCTACTACACTTANNTGNANNCANCACAAGATTNAACAAGNCATTAACACNNANCACAAGCATAAATTCGCGATTCTACTANTATTATTAACAATAATCACAAGNTNTAACACGTCATTAACACAAGTCACAAGCATAAATTAGCNATTTAGTAGGGNTTATNTGTATTGATCACAAGATCTAACAAGNCATTAACACAAGTCACAANCATAAATTAGCGAGTGAAAACGAACCGTGACAGGTCTNTCTGGCAATTGNATGCAAATAGAACAAAAGATTTCTAACTTCTTCTTTTCTTCATCATCGACACCATCGTCACCACCACCACTCATCAGNCTCTGCCTCTTTTTGGCTTTCTCAGCTTCGGTCAAAGTCACATCAGCCTGAATCGCACGGATCGCAGCAACGAGAACTGAACCGGAAGATTCAGGTNCGGAGATTCCGGTACCCGGNGCGGCGGAGGGAACGACNACGCCGGAGCAATCGGGACACTCCCANTCTCCAGTGGAAGAAGCGAGTGATTCAGGAAGGAGACANGACACGTGCCATGGAGTGACNCANGTGCCACANGTGAGAGTCTCTTCTGACGGAGGNNTCACCTGGCACCGCATACATACACCGTCTCCGTCGCAAGGAAGCTGAGTTTNAATCGCCATTGTTGAAACGAGNGNGNGAGNNAGAGAGAGNGNTGTTATCGAGAGATGAGAAGAGGAAGGGGAAAAATGAAGAGGCGNGTTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.054847
PRIMER_LEFT_0_PENALTY=0.042377
PRIMER_RIGHT_0_PENALTY=0.012470
PRIMER_LEFT_0_SEQUENCE=CTCCCAAGATTCTCCAACCA
PRIMER_RIGHT_0_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_0=419,20
PRIMER_RIGHT_0=1136,20
PRIMER_LEFT_0_TM=60.042
PRIMER_RIGHT_0_TM=60.012
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=718
PRIMER_PAIR_1_PENALTY=0.054847
PRIMER_LEFT_1_PENALTY=0.042377
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=CTCCCAAGATTCTCCAACCA
PRIMER_RIGHT_1_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_1=419,20
PRIMER_RIGHT_1=1137,20
PRIMER_LEFT_1_TM=60.042
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=719
PRIMER_PAIR_2_PENALTY=0.060729
PRIMER_LEFT_2_PENALTY=0.048259
PRIMER_RIGHT_2_PENALTY=0.012470
PRIMER_LEFT_2_SEQUENCE=TCCACTGAGATCCCTTCCAC
PRIMER_RIGHT_2_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_2=153,20
PRIMER_RIGHT_2=1136,20
PRIMER_LEFT_2_TM=60.048
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=984
PRIMER_PAIR_3_PENALTY=0.060729
PRIMER_LEFT_3_PENALTY=0.048259
PRIMER_RIGHT_3_PENALTY=0.012470
PRIMER_LEFT_3_SEQUENCE=TCCACTGAGATCCCTTCCAC
PRIMER_RIGHT_3_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_3=153,20
PRIMER_RIGHT_3=1137,20
PRIMER_LEFT_3_TM=60.048
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=985
PRIMER_PAIR_4_PENALTY=0.109325
PRIMER_LEFT_4_PENALTY=0.096855
PRIMER_RIGHT_4_PENALTY=0.012470
PRIMER_LEFT_4_SEQUENCE=CCCAAGATTCTCCAACCAAA
PRIMER_RIGHT_4_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_4=421,20
PRIMER_RIGHT_4=1136,20
PRIMER_LEFT_4_TM=59.903
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=716
PRIMER_PAIR_5_PENALTY=0.109325
PRIMER_LEFT_5_PENALTY=0.096855
PRIMER_RIGHT_5_PENALTY=0.012470
PRIMER_LEFT_5_SEQUENCE=CCCAAGATTCTCCAACCAAA
PRIMER_RIGHT_5_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_5=421,20
PRIMER_RIGHT_5=1137,20
PRIMER_LEFT_5_TM=59.903
PRIMER_RIGHT_5_TM=60.012
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.8000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=717
PRIMER_PAIR_6_PENALTY=0.114314
PRIMER_LEFT_6_PENALTY=0.042377
PRIMER_RIGHT_6_PENALTY=0.071937
PRIMER_LEFT_6_SEQUENCE=CTCCCAAGATTCTCCAACCA
PRIMER_RIGHT_6_SEQUENCE=CGATGGTGTCGATGATGAAG
PRIMER_LEFT_6=419,20
PRIMER_RIGHT_6=1208,20
PRIMER_LEFT_6_TM=60.042
PRIMER_RIGHT_6_TM=60.072
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=790
PRIMER_PAIR_7_PENALTY=0.117293
PRIMER_LEFT_7_PENALTY=0.042377
PRIMER_RIGHT_7_PENALTY=0.074917
PRIMER_LEFT_7_SEQUENCE=CTCCCAAGATTCTCCAACCA
PRIMER_RIGHT_7_SEQUENCE=ACGATGGTGTCGATGATGAA
PRIMER_LEFT_7=419,20
PRIMER_RIGHT_7=1209,20
PRIMER_LEFT_7_TM=60.042
PRIMER_RIGHT_7_TM=59.925
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=791
PRIMER_PAIR_8_PENALTY=0.138296
PRIMER_LEFT_8_PENALTY=0.125826
PRIMER_RIGHT_8_PENALTY=0.012470
PRIMER_LEFT_8_SEQUENCE=GCCTACACTCTTGCCTGTCC
PRIMER_RIGHT_8_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_8=400,20
PRIMER_RIGHT_8=1136,20
PRIMER_LEFT_8_TM=59.874
PRIMER_RIGHT_8_TM=60.012
PRIMER_LEFT_8_GC_PERCENT=60.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=737
PRIMER_PAIR_9_PENALTY=0.138296
PRIMER_LEFT_9_PENALTY=0.125826
PRIMER_RIGHT_9_PENALTY=0.012470
PRIMER_LEFT_9_SEQUENCE=GCCTACACTCTTGCCTGTCC
PRIMER_RIGHT_9_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_9=400,20
PRIMER_RIGHT_9=1137,20
PRIMER_LEFT_9_TM=59.874
PRIMER_RIGHT_9_TM=60.012
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=738
PRIMER_PAIR_10_PENALTY=0.168793
PRIMER_LEFT_10_PENALTY=0.096855
PRIMER_RIGHT_10_PENALTY=0.071937
PRIMER_LEFT_10_SEQUENCE=CCCAAGATTCTCCAACCAAA
PRIMER_RIGHT_10_SEQUENCE=CGATGGTGTCGATGATGAAG
PRIMER_LEFT_10=421,20
PRIMER_RIGHT_10=1208,20
PRIMER_LEFT_10_TM=59.903
PRIMER_RIGHT_10_TM=60.072
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.8000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=788
PRIMER_PAIR_11_PENALTY=0.171772
PRIMER_LEFT_11_PENALTY=0.096855
PRIMER_RIGHT_11_PENALTY=0.074917
PRIMER_LEFT_11_SEQUENCE=CCCAAGATTCTCCAACCAAA
PRIMER_RIGHT_11_SEQUENCE=ACGATGGTGTCGATGATGAA
PRIMER_LEFT_11=421,20
PRIMER_RIGHT_11=1209,20
PRIMER_LEFT_11_TM=59.903
PRIMER_RIGHT_11_TM=59.925
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.8000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=789
PRIMER_PAIR_12_PENALTY=0.197764
PRIMER_LEFT_12_PENALTY=0.125826
PRIMER_RIGHT_12_PENALTY=0.071937
PRIMER_LEFT_12_SEQUENCE=GCCTACACTCTTGCCTGTCC
PRIMER_RIGHT_12_SEQUENCE=CGATGGTGTCGATGATGAAG
PRIMER_LEFT_12=400,20
PRIMER_RIGHT_12=1208,20
PRIMER_LEFT_12_TM=59.874
PRIMER_RIGHT_12_TM=60.072
PRIMER_LEFT_12_GC_PERCENT=60.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=809
PRIMER_PAIR_13_PENALTY=0.198437
PRIMER_LEFT_13_PENALTY=0.042377
PRIMER_RIGHT_13_PENALTY=0.156061
PRIMER_LEFT_13_SEQUENCE=CTCCCAAGATTCTCCAACCA
PRIMER_RIGHT_13_SEQUENCE=TCTTCCGGTTCAGTTCTCGT
PRIMER_LEFT_13=419,20
PRIMER_RIGHT_13=1318,20
PRIMER_LEFT_13_TM=60.042
PRIMER_RIGHT_13_TM=59.844
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=900
PRIMER_PAIR_14_PENALTY=0.200743
PRIMER_LEFT_14_PENALTY=0.125826
PRIMER_RIGHT_14_PENALTY=0.074917
PRIMER_LEFT_14_SEQUENCE=GCCTACACTCTTGCCTGTCC
PRIMER_RIGHT_14_SEQUENCE=ACGATGGTGTCGATGATGAA
PRIMER_LEFT_14=400,20
PRIMER_RIGHT_14=1209,20
PRIMER_LEFT_14_TM=59.874
PRIMER_RIGHT_14_TM=59.925
PRIMER_LEFT_14_GC_PERCENT=60.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=810
PRIMER_PAIR_15_PENALTY=0.204150
PRIMER_LEFT_15_PENALTY=0.191680
PRIMER_RIGHT_15_PENALTY=0.012470
PRIMER_LEFT_15_SEQUENCE=GCTGGTATGGGACCAAAATG
PRIMER_RIGHT_15_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_15=471,20
PRIMER_RIGHT_15=1136,20
PRIMER_LEFT_15_TM=60.192
PRIMER_RIGHT_15_TM=60.012
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.2000
PRIMER_RIGHT_15_END_STABILITY=7.3000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=666
PRIMER_PAIR_16_PENALTY=0.204877
PRIMER_LEFT_16_PENALTY=0.042377
PRIMER_RIGHT_16_PENALTY=0.162500
PRIMER_LEFT_16_SEQUENCE=CTCCCAAGATTCTCCAACCA
PRIMER_RIGHT_16_SEQUENCE=GCTGATGTGACTTTGACCGA
PRIMER_LEFT_16=419,20
PRIMER_RIGHT_16=1276,20
PRIMER_LEFT_16_TM=60.042
PRIMER_RIGHT_16_TM=59.837
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=858
PRIMER_PAIR_17_PENALTY=0.215177
PRIMER_LEFT_17_PENALTY=0.202707
PRIMER_RIGHT_17_PENALTY=0.012470
PRIMER_LEFT_17_SEQUENCE=CTTCCACCACTGCAACACAC
PRIMER_RIGHT_17_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_17=166,20
PRIMER_RIGHT_17=1136,20
PRIMER_LEFT_17_TM=60.203
PRIMER_RIGHT_17_TM=60.012
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=971
PRIMER_PAIR_18_PENALTY=0.215177
PRIMER_LEFT_18_PENALTY=0.202707
PRIMER_RIGHT_18_PENALTY=0.012470
PRIMER_LEFT_18_SEQUENCE=CTTCCACCACTGCAACACAC
PRIMER_RIGHT_18_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_18=166,20
PRIMER_RIGHT_18=1137,20
PRIMER_LEFT_18_TM=60.203
PRIMER_RIGHT_18_TM=60.012
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=972
PRIMER_PAIR_19_PENALTY=0.227366
PRIMER_LEFT_19_PENALTY=0.214896
PRIMER_RIGHT_19_PENALTY=0.012470
PRIMER_LEFT_19_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_19_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_19=519,20
PRIMER_RIGHT_19=1136,20
PRIMER_LEFT_19_TM=59.785
PRIMER_RIGHT_19_TM=60.012
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=618
PRIMER_PAIR_20_PENALTY=0.227366
PRIMER_LEFT_20_PENALTY=0.214896
PRIMER_RIGHT_20_PENALTY=0.012470
PRIMER_LEFT_20_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_20_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_20=519,20
PRIMER_RIGHT_20=1137,20
PRIMER_LEFT_20_TM=59.785
PRIMER_RIGHT_20_TM=60.012
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=619
PRIMER_PAIR_21_PENALTY=0.233596
PRIMER_LEFT_21_PENALTY=0.214896
PRIMER_RIGHT_21_PENALTY=0.018700
PRIMER_LEFT_21_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_21_SEQUENCE=GTGTATGTATGCGGTGCCAG
PRIMER_LEFT_21=519,20
PRIMER_RIGHT_21=1512,20
PRIMER_LEFT_21_TM=59.785
PRIMER_RIGHT_21_TM=60.019
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=994
PRIMER_PAIR_22_PENALTY=0.241543
PRIMER_LEFT_22_PENALTY=0.191680
PRIMER_RIGHT_22_PENALTY=0.049863
PRIMER_LEFT_22_SEQUENCE=GCTGGTATGGGACCAAAATG
PRIMER_RIGHT_22_SEQUENCE=TCCTTCCTGAATCACTCGCT
PRIMER_LEFT_22=471,20
PRIMER_RIGHT_22=1425,20
PRIMER_LEFT_22_TM=60.192
PRIMER_RIGHT_22_TM=59.950
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.2000
PRIMER_RIGHT_22_END_STABILITY=9.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=955
PRIMER_PAIR_23_PENALTY=0.252916
PRIMER_LEFT_23_PENALTY=0.096855
PRIMER_RIGHT_23_PENALTY=0.156061
PRIMER_LEFT_23_SEQUENCE=CCCAAGATTCTCCAACCAAA
PRIMER_RIGHT_23_SEQUENCE=TCTTCCGGTTCAGTTCTCGT
PRIMER_LEFT_23=421,20
PRIMER_RIGHT_23=1318,20
PRIMER_LEFT_23_TM=59.903
PRIMER_RIGHT_23_TM=59.844
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.8000
PRIMER_RIGHT_23_END_STABILITY=8.1000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=898
PRIMER_PAIR_24_PENALTY=0.259355
PRIMER_LEFT_24_PENALTY=0.096855
PRIMER_RIGHT_24_PENALTY=0.162500
PRIMER_LEFT_24_SEQUENCE=CCCAAGATTCTCCAACCAAA
PRIMER_RIGHT_24_SEQUENCE=GCTGATGTGACTTTGACCGA
PRIMER_LEFT_24=421,20
PRIMER_RIGHT_24=1276,20
PRIMER_LEFT_24_TM=59.903
PRIMER_RIGHT_24_TM=59.837
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=856
PRIMER_PAIR_25_PENALTY=0.263618
PRIMER_LEFT_25_PENALTY=0.191680
PRIMER_RIGHT_25_PENALTY=0.071937
PRIMER_LEFT_25_SEQUENCE=GCTGGTATGGGACCAAAATG
PRIMER_RIGHT_25_SEQUENCE=CGATGGTGTCGATGATGAAG
PRIMER_LEFT_25=471,20
PRIMER_RIGHT_25=1208,20
PRIMER_LEFT_25_TM=60.192
PRIMER_RIGHT_25_TM=60.072
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.2000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=738
PRIMER_PAIR_26_PENALTY=0.264759
PRIMER_LEFT_26_PENALTY=0.214896
PRIMER_RIGHT_26_PENALTY=0.049863
PRIMER_LEFT_26_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_26_SEQUENCE=TCCTTCCTGAATCACTCGCT
PRIMER_LEFT_26=519,20
PRIMER_RIGHT_26=1425,20
PRIMER_LEFT_26_TM=59.785
PRIMER_RIGHT_26_TM=59.950
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=907
PRIMER_PAIR_27_PENALTY=0.266597
PRIMER_LEFT_27_PENALTY=0.191680
PRIMER_RIGHT_27_PENALTY=0.074917
PRIMER_LEFT_27_SEQUENCE=GCTGGTATGGGACCAAAATG
PRIMER_RIGHT_27_SEQUENCE=ACGATGGTGTCGATGATGAA
PRIMER_LEFT_27=471,20
PRIMER_RIGHT_27=1209,20
PRIMER_LEFT_27_TM=60.192
PRIMER_RIGHT_27_TM=59.925
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.2000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=739
PRIMER_PAIR_28_PENALTY=0.281887
PRIMER_LEFT_28_PENALTY=0.125826
PRIMER_RIGHT_28_PENALTY=0.156061
PRIMER_LEFT_28_SEQUENCE=GCCTACACTCTTGCCTGTCC
PRIMER_RIGHT_28_SEQUENCE=TCTTCCGGTTCAGTTCTCGT
PRIMER_LEFT_28=400,20
PRIMER_RIGHT_28=1318,20
PRIMER_LEFT_28_TM=59.874
PRIMER_RIGHT_28_TM=59.844
PRIMER_LEFT_28_GC_PERCENT=60.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=919
PRIMER_PAIR_29_PENALTY=0.286833
PRIMER_LEFT_29_PENALTY=0.214896
PRIMER_RIGHT_29_PENALTY=0.071937
PRIMER_LEFT_29_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_29_SEQUENCE=CGATGGTGTCGATGATGAAG
PRIMER_LEFT_29=519,20
PRIMER_RIGHT_29=1208,20
PRIMER_LEFT_29_TM=59.785
PRIMER_RIGHT_29_TM=60.072
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=690
PRIMER_PAIR_30_PENALTY=0.288326
PRIMER_LEFT_30_PENALTY=0.125826
PRIMER_RIGHT_30_PENALTY=0.162500
PRIMER_LEFT_30_SEQUENCE=GCCTACACTCTTGCCTGTCC
PRIMER_RIGHT_30_SEQUENCE=GCTGATGTGACTTTGACCGA
PRIMER_LEFT_30=400,20
PRIMER_RIGHT_30=1276,20
PRIMER_LEFT_30_TM=59.874
PRIMER_RIGHT_30_TM=59.837
PRIMER_LEFT_30_GC_PERCENT=60.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=9.6000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=877
PRIMER_PAIR_31_PENALTY=0.289813
PRIMER_LEFT_31_PENALTY=0.214896
PRIMER_RIGHT_31_PENALTY=0.074917
PRIMER_LEFT_31_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_31_SEQUENCE=ACGATGGTGTCGATGATGAA
PRIMER_LEFT_31=519,20
PRIMER_RIGHT_31=1209,20
PRIMER_LEFT_31_TM=59.785
PRIMER_RIGHT_31_TM=59.925
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=691
PRIMER_PAIR_32_PENALTY=0.291317
PRIMER_LEFT_32_PENALTY=0.278847
PRIMER_RIGHT_32_PENALTY=0.012470
PRIMER_LEFT_32_SEQUENCE=TCCCCACTGCCTACACTCTT
PRIMER_RIGHT_32_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_32=392,20
PRIMER_RIGHT_32=1136,20
PRIMER_LEFT_32_TM=59.721
PRIMER_RIGHT_32_TM=60.012
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=745
PRIMER_PAIR_33_PENALTY=0.291317
PRIMER_LEFT_33_PENALTY=0.278847
PRIMER_RIGHT_33_PENALTY=0.012470
PRIMER_LEFT_33_SEQUENCE=TCCCCACTGCCTACACTCTT
PRIMER_RIGHT_33_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_33=392,20
PRIMER_RIGHT_33=1137,20
PRIMER_LEFT_33_TM=59.721
PRIMER_RIGHT_33_TM=60.012
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=746
PRIMER_PAIR_34_PENALTY=0.329829
PRIMER_LEFT_34_PENALTY=0.317359
PRIMER_RIGHT_34_PENALTY=0.012470
PRIMER_LEFT_34_SEQUENCE=GGACCAAAATGATCACGAGG
PRIMER_RIGHT_34_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_34=480,20
PRIMER_RIGHT_34=1136,20
PRIMER_LEFT_34_TM=60.317
PRIMER_RIGHT_34_TM=60.012
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.9000
PRIMER_RIGHT_34_END_STABILITY=7.3000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=657
PRIMER_PAIR_35_PENALTY=0.343966
PRIMER_LEFT_35_PENALTY=0.214896
PRIMER_RIGHT_35_PENALTY=0.129070
PRIMER_LEFT_35_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_35_SEQUENCE=GACGGTGTATGTATGCGGTG
PRIMER_LEFT_35=519,20
PRIMER_RIGHT_35=1516,20
PRIMER_LEFT_35_TM=59.785
PRIMER_RIGHT_35_TM=59.871
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=998
PRIMER_PAIR_36_PENALTY=0.347741
PRIMER_LEFT_36_PENALTY=0.191680
PRIMER_RIGHT_36_PENALTY=0.156061
PRIMER_LEFT_36_SEQUENCE=GCTGGTATGGGACCAAAATG
PRIMER_RIGHT_36_SEQUENCE=TCTTCCGGTTCAGTTCTCGT
PRIMER_LEFT_36=471,20
PRIMER_RIGHT_36=1318,20
PRIMER_LEFT_36_TM=60.192
PRIMER_RIGHT_36_TM=59.844
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.2000
PRIMER_RIGHT_36_END_STABILITY=8.1000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=848
PRIMER_PAIR_37_PENALTY=0.350785
PRIMER_LEFT_37_PENALTY=0.278847
PRIMER_RIGHT_37_PENALTY=0.071937
PRIMER_LEFT_37_SEQUENCE=TCCCCACTGCCTACACTCTT
PRIMER_RIGHT_37_SEQUENCE=CGATGGTGTCGATGATGAAG
PRIMER_LEFT_37=392,20
PRIMER_RIGHT_37=1208,20
PRIMER_LEFT_37_TM=59.721
PRIMER_RIGHT_37_TM=60.072
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=817
PRIMER_PAIR_38_PENALTY=0.353764
PRIMER_LEFT_38_PENALTY=0.278847
PRIMER_RIGHT_38_PENALTY=0.074917
PRIMER_LEFT_38_SEQUENCE=TCCCCACTGCCTACACTCTT
PRIMER_RIGHT_38_SEQUENCE=ACGATGGTGTCGATGATGAA
PRIMER_LEFT_38=392,20
PRIMER_RIGHT_38=1209,20
PRIMER_LEFT_38_TM=59.721
PRIMER_RIGHT_38_TM=59.925
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=818
PRIMER_PAIR_39_PENALTY=0.354180
PRIMER_LEFT_39_PENALTY=0.191680
PRIMER_RIGHT_39_PENALTY=0.162500
PRIMER_LEFT_39_SEQUENCE=GCTGGTATGGGACCAAAATG
PRIMER_RIGHT_39_SEQUENCE=GCTGATGTGACTTTGACCGA
PRIMER_LEFT_39=471,20
PRIMER_RIGHT_39=1276,20
PRIMER_LEFT_39_TM=60.192
PRIMER_RIGHT_39_TM=59.837
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.2000
PRIMER_RIGHT_39_END_STABILITY=9.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=806
PRIMER_PAIR_40_PENALTY=0.367221
PRIMER_LEFT_40_PENALTY=0.317359
PRIMER_RIGHT_40_PENALTY=0.049863
PRIMER_LEFT_40_SEQUENCE=GGACCAAAATGATCACGAGG
PRIMER_RIGHT_40_SEQUENCE=TCCTTCCTGAATCACTCGCT
PRIMER_LEFT_40=480,20
PRIMER_RIGHT_40=1425,20
PRIMER_LEFT_40_TM=60.317
PRIMER_RIGHT_40_TM=59.950
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=5.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=9.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=946
PRIMER_PAIR_41_PENALTY=0.370957
PRIMER_LEFT_41_PENALTY=0.214896
PRIMER_RIGHT_41_PENALTY=0.156061
PRIMER_LEFT_41_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_41_SEQUENCE=TCTTCCGGTTCAGTTCTCGT
PRIMER_LEFT_41=519,20
PRIMER_RIGHT_41=1318,20
PRIMER_LEFT_41_TM=59.785
PRIMER_RIGHT_41_TM=59.844
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=8.1000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=800
PRIMER_PAIR_42_PENALTY=0.377396
PRIMER_LEFT_42_PENALTY=0.214896
PRIMER_RIGHT_42_PENALTY=0.162500
PRIMER_LEFT_42_SEQUENCE=CCGCTAGCAGCATTAGCTTT
PRIMER_RIGHT_42_SEQUENCE=GCTGATGTGACTTTGACCGA
PRIMER_LEFT_42=519,20
PRIMER_RIGHT_42=1276,20
PRIMER_LEFT_42_TM=59.785
PRIMER_RIGHT_42_TM=59.837
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=9.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=758
PRIMER_PAIR_43_PENALTY=0.389296
PRIMER_LEFT_43_PENALTY=0.317359
PRIMER_RIGHT_43_PENALTY=0.071937
PRIMER_LEFT_43_SEQUENCE=GGACCAAAATGATCACGAGG
PRIMER_RIGHT_43_SEQUENCE=CGATGGTGTCGATGATGAAG
PRIMER_LEFT_43=480,20
PRIMER_RIGHT_43=1208,20
PRIMER_LEFT_43_TM=60.317
PRIMER_RIGHT_43_TM=60.072
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=729
PRIMER_PAIR_44_PENALTY=0.392275
PRIMER_LEFT_44_PENALTY=0.317359
PRIMER_RIGHT_44_PENALTY=0.074917
PRIMER_LEFT_44_SEQUENCE=GGACCAAAATGATCACGAGG
PRIMER_RIGHT_44_SEQUENCE=ACGATGGTGTCGATGATGAA
PRIMER_LEFT_44=480,20
PRIMER_RIGHT_44=1209,20
PRIMER_LEFT_44_TM=60.317
PRIMER_RIGHT_44_TM=59.925
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.9000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=730
PRIMER_PAIR_45_PENALTY=0.397886
PRIMER_LEFT_45_PENALTY=0.385416
PRIMER_RIGHT_45_PENALTY=0.012470
PRIMER_LEFT_45_SEQUENCE=AGCAGCATTAGCTTTCCCAG
PRIMER_RIGHT_45_SEQUENCE=ACCTGTCACGGTTCGTTTTC
PRIMER_LEFT_45=524,20
PRIMER_RIGHT_45=1136,20
PRIMER_LEFT_45_TM=59.615
PRIMER_RIGHT_45_TM=60.012
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.7000
PRIMER_RIGHT_45_END_STABILITY=7.3000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=613
PRIMER_PAIR_46_PENALTY=0.397886
PRIMER_LEFT_46_PENALTY=0.385416
PRIMER_RIGHT_46_PENALTY=0.012470
PRIMER_LEFT_46_SEQUENCE=AGCAGCATTAGCTTTCCCAG
PRIMER_RIGHT_46_SEQUENCE=GACCTGTCACGGTTCGTTTT
PRIMER_LEFT_46=524,20
PRIMER_RIGHT_46=1137,20
PRIMER_LEFT_46_TM=59.615
PRIMER_RIGHT_46_TM=60.012
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=614
PRIMER_PAIR_47_PENALTY=0.404116
PRIMER_LEFT_47_PENALTY=0.385416
PRIMER_RIGHT_47_PENALTY=0.018700
PRIMER_LEFT_47_SEQUENCE=AGCAGCATTAGCTTTCCCAG
PRIMER_RIGHT_47_SEQUENCE=GTGTATGTATGCGGTGCCAG
PRIMER_LEFT_47=524,20
PRIMER_RIGHT_47=1512,20
PRIMER_LEFT_47_TM=59.615
PRIMER_RIGHT_47_TM=60.019
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=989
PRIMER_PAIR_48_PENALTY=0.420209
PRIMER_LEFT_48_PENALTY=0.042377
PRIMER_RIGHT_48_PENALTY=0.377832
PRIMER_LEFT_48_SEQUENCE=CTCCCAAGATTCTCCAACCA
PRIMER_RIGHT_48_SEQUENCE=AGACCTGTCACGGTTCGTTT
PRIMER_LEFT_48=419,20
PRIMER_RIGHT_48=1138,20
PRIMER_LEFT_48_TM=60.042
PRIMER_RIGHT_48_TM=59.622
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=8.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=720
PRIMER_PAIR_49_PENALTY=0.426091
PRIMER_LEFT_49_PENALTY=0.048259
PRIMER_RIGHT_49_PENALTY=0.377832
PRIMER_LEFT_49_SEQUENCE=TCCACTGAGATCCCTTCCAC
PRIMER_RIGHT_49_SEQUENCE=AGACCTGTCACGGTTCGTTT
PRIMER_LEFT_49=153,20
PRIMER_RIGHT_49=1138,20
PRIMER_LEFT_49_TM=60.048
PRIMER_RIGHT_49_TM=59.622
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=8.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=986
=
SEQUENCE_ID=MAGIC.105_5_14103569_14104244_zu-0_hi-0_9259_166624_TRUE
SEQUENCE_TARGET=950,775
SEQUENCE_TEMPLATE=CAGTTTTGTGNGTGTCCTCCTCTTTCAAACAAACTTTTCTNCCACATATTGGGTTGATGCTTTAAACATGATTGTCCATCTATTTAATATTCTTCCCTCGAGCNTCAAAAACAANAANATTNTTTTTACTTTGCTNTTTAACTNAAACATTTTNNTTNTGATCATCTTCGNGNTTTTAGTTNNNTNTNCNNNNCATATCGTCTACNAAATTTGGCTAACAAGCTCTTACCTTGCTTCAATCAATGNNNNTATCTTTCTCGGTNNNNTATCCTTTGAACTATCGCGGTNGCTATTTCATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTAATCATCATCTCTCCTTACTTACNNACTAATCCNTCTTATTCTATCTATAATTTANGCTCTTTCTCANACAGCTACAATCCCTCATGTATATCNCTTTGTCTCACNATCACCAATCACTCCACCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGATTATACATCANCACTCCACATTGCCACATTTGATTCTTCAGCTCTCNTTATNNCTCTATCGCAGCATAATAGGTATTATTCGTTATAGTAGTTAAAACAGTTTACTTTTAACCCAAGTAACATATNCNTACTTTGATACTAGAAATGATTCTNNNNNNNNNNNNNNNNNNNNNNNNNNGATTCAAACTAANTAATGACTAGATTCGAATAGCTACTACGTACGNGATGGCACTAATGGTGAAAGCTGAAGGTGGAAATGGAGGGAAGCNATGGGATGATGGATTCGATTATGAGGGTGTAACAAAGATATATGTACGAGGAGGTCTTGAAGGCATNCAGTTCATCNAGTTNNACTATNTCAAAGATGGTAAAACTATTNCTGGACCAATNCATGGTGTCTCTGGTCGTGGTTTGACACAAACGGTACATANGTAANNNNNNNNNNNNNNNNNTNTATATCTNATTTNACAATCNTTTTTNTATATATATNNNNNNNNNNTTTTTGCAGTTTGAGATTAACNATTNG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_4923600_4924133_sf-2_wil-2_116280_45367_TRUE
SEQUENCE_TARGET=950,633
SEQUENCE_TEMPLATE=TTTGGCGATTGTACTTTGATACTGAGTGTTTTTCCTCTTTGAAATGAATAATAATAAAGTATCTTTTCCTGTTGATTAATTAATCTCTGAAATACTCATTATNTNNNGTTGAACTCAAGGTAACGTTACACATAGAACTTGTGATGTTCAAAAACAAAGCATAAAGCAAATGAGTGACAATGTTTTCTTTTGAAAAATCGTTTTATGTGAAATCATCAGAGGCATTGGAGATTTATGAATCAGAACATCTTGCAGAAATCATGCACTGAGAGGCACAAATTGGATGCCATGCCGAAATGAGGGCATCTCGATTCACGTATTGTGGGCCGAATAGTAAGGCCCATGGAAGATTAAAGTCAATTCAAATTCGAACGTTGAAAGCATTAAATCCAAAGACGGANNNNNNNNNNTTAAAAGAGAATTATGGTGTATAATTAAGAATAATTACTAGGTTTTTGTATAAATAAGAGAGGTCTTGGACCATGTAAGACACCCACAATTTTTCTAGCAATTTTNGATAGTCATTTTGATATCAATTTTTAGCTTTTACATTTTATNTTTGTCCTCNAAAATTAATCCATCTCACAAAANAAATAAATACTTGAGCGTGTGAATCCGACATCCACAACACTTTTGCCTGAAAAAATAATTAACATAAATGTAAAGTATCCTAACTTTCCCATTTTAAANNNNNNNNNNNNNNNNNNNTATCTTTAAAANGTTTTGANANATNTACACCCAAAANTATATTTTTGAAAAATCTAAAGTTGNNNNNNNNTAAGGTAATATATCTAAGGTAGACTAAAGATATATTNAATAACAAATAAAAAATCTNAAGTTTTTAAATNACTCNAAATTGNACGGTATANATGAANTCTAACNCCTATCATNTATNTATNTANANGACAANGGTNTTTCNTATTTATNTCGATANCNANACTATCCTCAGCTTGTGTAATCCTCANNTTGCNCCTGACTTTTNAACNGTCAAATAATATGANCCGTTCGATNGAATATGAGATGAGNATTTTNAAATCGTAACNGTNCGTTNTTAACTTTGGNCATTGNCCNTGCTCTTTANTTTTATAATTAATTAATTGAANCCAAATAATTNTGACCNTCNGATCGATATNAAAAGNAANGAGTTGATAACATNCGTCCGATTTTANCAAAAANNANGAACGAATNTNAGCCGTCCATTTTNTACCCTTCANTAAACCCTAATACTCGTACCTAATTAACCTAATATAAATATAAAANTACGGTGCCNCTTNNNNNNTTGTCTATCCTATTTGCNCCTAAGAACTACTCNNNNNNNNNNNAGGTTTNGTNTACAAGATTGGGATCTCATNATTGACAANGAGAAAGGTAATTTAGTCAAGGNCNATAGATTTAANTATGCGAAGAGAACNNNNNNNNNNNNCANATTTGGGTATGTGAAGAGAGCCATGCACGGTACAAAGATAGTTATCAAATAAAGCTGTCAAGTNTATTGACTTTTCAACACCATTNGTAATGTGAAGAAANAAGAGANTTGCAAAGTCCTCCTTCTTTATTNGAAGGAAATCTCATTCTCTTATTGTCTTTGTATGGGTGAAAGTNANATCTATNGAAGNGAGTTAGTTGACCTGCGGANCCAGAGTCGATGGGAGACGCTGAAGCGGTTGGAAACGTTTCGAGTTGNGCAAGAACTTGGTCTAGGTTTGTTCGAAGAGTATTAGGGTTTGCTTTGTTAGTTTGATCGATACCACGCCAAATCTGATATTNGACGTAAAGACTNTTCACCTTCTCTTGTAAAACCCAATTTAGGTTTCGAANNNNNACNGTNNNTTGTGAAGTTACTANCGCNGTTTGNTAACACTTAAGTGNANNGTCNAACTCTCGCTGATTNTTACATATCANCAAATTTTTCTNCNTNCTTTATATCATCTAACTAGCTGATTTGAGATTCACAATCTTGATTCTCCAGGTTGCTCTGTATATTGGTGGTCTTTATGTTTGTGGAAAGGTTGGATTTTGAACTCTTATTCTTGCAAACAATGATCTTAGCTTTTGATTTGAAGCCATTGTTNGAGTTTTCGGTCTTGGTAATATACAACAATGTTAAAAATATGCAGGTTGGATTGGAATCTNTAATGAAAATGGGANTANAGACAAGAGAACTGTTCTTNTATGANACATTTCTGTATTNTAACNCGCTCCTTCTCATTGTAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGCTTNTTCTCGGTCTGGAGTTGATTATGCAGCAATCTTTTATCNANGACCAGATCATCTTAGTCACAAAGAGATATGGAAGGTAAACNACTNTTACTTANATCATTTGTATATNTTTTTCATTCGATTATTACATCAATGTAACNNATGTCACANTGTGCCNGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17809876_17809985_ct-1_tsu-0_15956_506749_TRUE
SEQUENCE_TARGET=950,209
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGAAGCGGTAGTTGGGCCTTTTTCTCTCCTTTCAAAATATATAATGGGCTTTTTAGTTCNCGGAAGACCTAGAGCTTGGAATCGTCTGTAATATACTCCACACTAAAGTATATCTANCACTAAANAAATCCAACGGCTGAGAGTCTTTTATTCACTATAACACGTGATAGTTCTCCCTAACTCGTAAGGATAATAATCTGAAGGAGTGATGAGAAAATATTAACGAAAAANAAAAAAAAAAAGACAGAAGANAAAAAAAAATCACGAAATCTGATTTTCCTCTCTTTTGCTCGAAGCCAAGTCTCTGCTTTTACTTTGATCCACCAACTTCATCTTCAACCTCTCGATTTTAGGTACGTTTTTCCCTTTCTTTGTGTATCTTANAAGTCTTTTGATTNTCACGCTGATTCATTTCTGNAAGTTTTTCGATTTGGTCTTCATAGATTCCTGAAATCGTTAGTTTGAAATTGATTGAGATCTTTGATTCTATATNNAAANNTTATCTGATCTGTTTATTCTGGAATNAATTTGTGATTAGAGGAAATTGTGTCCTCTGTTTTAANTTTCTGATTTTGGATTTGAAGATCCGATTTTTGCATCTTGATGATTCATTTCAAAAAATGTCTAATTTTGGNATGATTNTTGCTTGTCTCAGGTNAGATTCTTAGAGTGNTTTTGACTTATCTAAGAATGGAGCTAAAGAGCATTAAGGATGCGTTTGATCGTGTTGCGACGAAGCAGAAGCTCTCTTATAGCAAAACTAATGAGATTGTTCATATGTTGTCTCAAGAAATCGANAAGGCTTTGAGCATATTAGAGGAGACACCATCATCAGATACTATGCTTCTTGATCATAGATCTATNCTCGCTGATNTGAAGAAAGTGTTTATGGAGATTGCTCCNATCACTCAACTTGAAGCTACTGAGAAAGAGCTACATGCGGCTTTGACCAAGTACCCTAAAGTTCTTGAGAAACAGCTGAATCCAGACATANCAAAGGCTTATAGACACAATGTTGAGTTNGATACTCATATCGTTAACCAGATTATCGCCAACTTTTTCTACCGTCAAGGAATGTTTGACATTGGTGACTGTTTTGTTGCTGAAACTGGTGAATCTGAATGTTCNACAAGACAATCTTTCGTGGAGATGTATCGGATACTAGAAGCTATGAAGAGACGAGATCTTGAACCGGCTCTTAATTGGGCTGTTTCGAACTCTGACAAACTAAAGNAAGCAAGGTCTGATCTTGAGATGAAGCTTCATAGTCTACACTTTCTGGAAATAGCNCGAGGCAAAAACTCCAAAGAAGCTATCGACTANGCGAGAAANCATATCGCCACGTTTGCAGATAGCTGCCTCCCGGAGATCCAGAAGCTCATGTGTTCTCTCTTATGGAACAGAAANCTCGATAAATCACCATACTCNGAGTTTCTCTCCCCCNCTNTGTGGAACAATGCAGTCAAAGAGCTAACCCGACAGTACTGCAACC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.257561
PRIMER_LEFT_0_PENALTY=0.132262
PRIMER_RIGHT_0_PENALTY=0.125300
PRIMER_LEFT_0_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_0_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_0=900,20
PRIMER_RIGHT_0=1560,20
PRIMER_LEFT_0_TM=60.132
PRIMER_RIGHT_0_TM=60.125
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=661
PRIMER_PAIR_1_PENALTY=0.281656
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.149395
PRIMER_LEFT_1_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_1_SEQUENCE=GTCAGAGTTCGAAACAGCCC
PRIMER_LEFT_1=900,20
PRIMER_RIGHT_1=1840,20
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=59.851
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=941
PRIMER_PAIR_2_PENALTY=0.304894
PRIMER_LEFT_2_PENALTY=0.179594
PRIMER_RIGHT_2_PENALTY=0.125300
PRIMER_LEFT_2_SEQUENCE=TGGGCCTTTTTCTCTCCTTT
PRIMER_RIGHT_2_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_2=629,20
PRIMER_RIGHT_2=1560,20
PRIMER_LEFT_2_TM=60.180
PRIMER_RIGHT_2_TM=60.125
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=932
PRIMER_PAIR_3_PENALTY=0.304894
PRIMER_LEFT_3_PENALTY=0.179594
PRIMER_RIGHT_3_PENALTY=0.125300
PRIMER_LEFT_3_SEQUENCE=TTGGGCCTTTTTCTCTCCTT
PRIMER_RIGHT_3_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_3=628,20
PRIMER_RIGHT_3=1560,20
PRIMER_LEFT_3_TM=60.180
PRIMER_RIGHT_3_TM=60.125
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=933
PRIMER_PAIR_4_PENALTY=0.402112
PRIMER_LEFT_4_PENALTY=0.276812
PRIMER_RIGHT_4_PENALTY=0.125300
PRIMER_LEFT_4_SEQUENCE=TTGCTCGAAGCCAAGTCTCT
PRIMER_RIGHT_4_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_4=903,20
PRIMER_RIGHT_4=1560,20
PRIMER_LEFT_4_TM=60.277
PRIMER_RIGHT_4_TM=60.125
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=658
PRIMER_PAIR_5_PENALTY=0.426207
PRIMER_LEFT_5_PENALTY=0.276812
PRIMER_RIGHT_5_PENALTY=0.149395
PRIMER_LEFT_5_SEQUENCE=TTGCTCGAAGCCAAGTCTCT
PRIMER_RIGHT_5_SEQUENCE=GTCAGAGTTCGAAACAGCCC
PRIMER_LEFT_5=903,20
PRIMER_RIGHT_5=1840,20
PRIMER_LEFT_5_TM=60.277
PRIMER_RIGHT_5_TM=59.851
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=10.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=938
PRIMER_PAIR_6_PENALTY=0.434717
PRIMER_LEFT_6_PENALTY=0.309418
PRIMER_RIGHT_6_PENALTY=0.125300
PRIMER_LEFT_6_SEQUENCE=GTTGGGCCTTTTTCTCTCCT
PRIMER_RIGHT_6_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_6=627,20
PRIMER_RIGHT_6=1560,20
PRIMER_LEFT_6_TM=59.691
PRIMER_RIGHT_6_TM=60.125
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=934
PRIMER_PAIR_7_PENALTY=0.434717
PRIMER_LEFT_7_PENALTY=0.309418
PRIMER_RIGHT_7_PENALTY=0.125300
PRIMER_LEFT_7_SEQUENCE=AGTTGGGCCTTTTTCTCTCC
PRIMER_RIGHT_7_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_7=626,20
PRIMER_RIGHT_7=1560,20
PRIMER_LEFT_7_TM=59.691
PRIMER_RIGHT_7_TM=60.125
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=935
PRIMER_PAIR_8_PENALTY=0.599772
PRIMER_LEFT_8_PENALTY=0.474473
PRIMER_RIGHT_8_PENALTY=0.125300
PRIMER_LEFT_8_SEQUENCE=CGGTAGTTGGGCCTTTTTCT
PRIMER_RIGHT_8_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_8=622,20
PRIMER_RIGHT_8=1560,20
PRIMER_LEFT_8_TM=60.474
PRIMER_RIGHT_8_TM=60.125
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=939
PRIMER_PAIR_9_PENALTY=0.652761
PRIMER_LEFT_9_PENALTY=0.527462
PRIMER_RIGHT_9_PENALTY=0.125300
PRIMER_LEFT_9_SEQUENCE=CCAACGGCTGAGAGTCTTTT
PRIMER_RIGHT_9_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_9=746,20
PRIMER_RIGHT_9=1560,20
PRIMER_LEFT_9_TM=59.473
PRIMER_RIGHT_9_TM=60.125
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=815
PRIMER_PAIR_10_PENALTY=0.658180
PRIMER_LEFT_10_PENALTY=0.132262
PRIMER_RIGHT_10_PENALTY=0.525918
PRIMER_LEFT_10_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_10_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_10=900,20
PRIMER_RIGHT_10=1345,20
PRIMER_LEFT_10_TM=60.132
PRIMER_RIGHT_10_TM=60.526
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.8000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=446
PRIMER_PAIR_11_PENALTY=0.705512
PRIMER_LEFT_11_PENALTY=0.179594
PRIMER_RIGHT_11_PENALTY=0.525918
PRIMER_LEFT_11_SEQUENCE=TGGGCCTTTTTCTCTCCTTT
PRIMER_RIGHT_11_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_11=629,20
PRIMER_RIGHT_11=1345,20
PRIMER_LEFT_11_TM=60.180
PRIMER_RIGHT_11_TM=60.526
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.8000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=717
PRIMER_PAIR_12_PENALTY=0.705512
PRIMER_LEFT_12_PENALTY=0.179594
PRIMER_RIGHT_12_PENALTY=0.525918
PRIMER_LEFT_12_SEQUENCE=TTGGGCCTTTTTCTCTCCTT
PRIMER_RIGHT_12_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_12=628,20
PRIMER_RIGHT_12=1345,20
PRIMER_LEFT_12_TM=60.180
PRIMER_RIGHT_12_TM=60.526
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.8000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=718
PRIMER_PAIR_13_PENALTY=0.782393
PRIMER_LEFT_13_PENALTY=0.657094
PRIMER_RIGHT_13_PENALTY=0.125300
PRIMER_LEFT_13_SEQUENCE=TTTGCTCGAAGCCAAGTCTC
PRIMER_RIGHT_13_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_13=902,20
PRIMER_RIGHT_13=1560,20
PRIMER_LEFT_13_TM=60.657
PRIMER_RIGHT_13_TM=60.125
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=659
PRIMER_PAIR_14_PENALTY=0.802730
PRIMER_LEFT_14_PENALTY=0.276812
PRIMER_RIGHT_14_PENALTY=0.525918
PRIMER_LEFT_14_SEQUENCE=TTGCTCGAAGCCAAGTCTCT
PRIMER_RIGHT_14_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_14=903,20
PRIMER_RIGHT_14=1345,20
PRIMER_LEFT_14_TM=60.277
PRIMER_RIGHT_14_TM=60.526
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.8000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=443
PRIMER_PAIR_15_PENALTY=0.806488
PRIMER_LEFT_15_PENALTY=0.657094
PRIMER_RIGHT_15_PENALTY=0.149395
PRIMER_LEFT_15_SEQUENCE=TTTGCTCGAAGCCAAGTCTC
PRIMER_RIGHT_15_SEQUENCE=GTCAGAGTTCGAAACAGCCC
PRIMER_LEFT_15=902,20
PRIMER_RIGHT_15=1840,20
PRIMER_LEFT_15_TM=60.657
PRIMER_RIGHT_15_TM=59.851
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=10.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=939
PRIMER_PAIR_16_PENALTY=0.835335
PRIMER_LEFT_16_PENALTY=0.309418
PRIMER_RIGHT_16_PENALTY=0.525918
PRIMER_LEFT_16_SEQUENCE=GTTGGGCCTTTTTCTCTCCT
PRIMER_RIGHT_16_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_16=627,20
PRIMER_RIGHT_16=1345,20
PRIMER_LEFT_16_TM=59.691
PRIMER_RIGHT_16_TM=60.526
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.8000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=719
PRIMER_PAIR_17_PENALTY=0.835335
PRIMER_LEFT_17_PENALTY=0.309418
PRIMER_RIGHT_17_PENALTY=0.525918
PRIMER_LEFT_17_SEQUENCE=AGTTGGGCCTTTTTCTCTCC
PRIMER_RIGHT_17_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_17=626,20
PRIMER_RIGHT_17=1345,20
PRIMER_LEFT_17_TM=59.691
PRIMER_RIGHT_17_TM=60.526
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.8000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=720
PRIMER_PAIR_18_PENALTY=0.840986
PRIMER_LEFT_18_PENALTY=0.715687
PRIMER_RIGHT_18_PENALTY=0.125300
PRIMER_LEFT_18_SEQUENCE=GGGCCTTTTTCTCTCCTTTC
PRIMER_RIGHT_18_SEQUENCE=GCCGCATGTAGCTCTTTCTC
PRIMER_LEFT_18=630,20
PRIMER_RIGHT_18=1560,20
PRIMER_LEFT_18_TM=59.284
PRIMER_RIGHT_18_TM=60.125
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=931
PRIMER_PAIR_19_PENALTY=0.885911
PRIMER_LEFT_19_PENALTY=0.132262
PRIMER_RIGHT_19_PENALTY=0.753650
PRIMER_LEFT_19_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_19_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_19=900,20
PRIMER_RIGHT_19=1561,20
PRIMER_LEFT_19_TM=60.132
PRIMER_RIGHT_19_TM=59.246
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=662
PRIMER_PAIR_20_PENALTY=0.933244
PRIMER_LEFT_20_PENALTY=0.179594
PRIMER_RIGHT_20_PENALTY=0.753650
PRIMER_LEFT_20_SEQUENCE=TGGGCCTTTTTCTCTCCTTT
PRIMER_RIGHT_20_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_20=629,20
PRIMER_RIGHT_20=1561,20
PRIMER_LEFT_20_TM=60.180
PRIMER_RIGHT_20_TM=59.246
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=933
PRIMER_PAIR_21_PENALTY=0.933244
PRIMER_LEFT_21_PENALTY=0.179594
PRIMER_RIGHT_21_PENALTY=0.753650
PRIMER_LEFT_21_SEQUENCE=TTGGGCCTTTTTCTCTCCTT
PRIMER_RIGHT_21_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_21=628,20
PRIMER_RIGHT_21=1561,20
PRIMER_LEFT_21_TM=60.180
PRIMER_RIGHT_21_TM=59.246
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=934
PRIMER_PAIR_22_PENALTY=0.958534
PRIMER_LEFT_22_PENALTY=0.132262
PRIMER_RIGHT_22_PENALTY=0.826272
PRIMER_LEFT_22_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_22_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_22=900,20
PRIMER_RIGHT_22=1559,20
PRIMER_LEFT_22_TM=60.132
PRIMER_RIGHT_22_TM=59.174
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=660
PRIMER_PAIR_23_PENALTY=1.000391
PRIMER_LEFT_23_PENALTY=0.474473
PRIMER_RIGHT_23_PENALTY=0.525918
PRIMER_LEFT_23_SEQUENCE=CGGTAGTTGGGCCTTTTTCT
PRIMER_RIGHT_23_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_23=622,20
PRIMER_RIGHT_23=1345,20
PRIMER_LEFT_23_TM=60.474
PRIMER_RIGHT_23_TM=60.526
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=7.8000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=724
PRIMER_PAIR_24_PENALTY=1.005867
PRIMER_LEFT_24_PENALTY=0.179594
PRIMER_RIGHT_24_PENALTY=0.826272
PRIMER_LEFT_24_SEQUENCE=TGGGCCTTTTTCTCTCCTTT
PRIMER_RIGHT_24_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_24=629,20
PRIMER_RIGHT_24=1559,20
PRIMER_LEFT_24_TM=60.180
PRIMER_RIGHT_24_TM=59.174
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=2.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=931
PRIMER_PAIR_25_PENALTY=1.005867
PRIMER_LEFT_25_PENALTY=0.179594
PRIMER_RIGHT_25_PENALTY=0.826272
PRIMER_LEFT_25_SEQUENCE=TTGGGCCTTTTTCTCTCCTT
PRIMER_RIGHT_25_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_25=628,20
PRIMER_RIGHT_25=1559,20
PRIMER_LEFT_25_TM=60.180
PRIMER_RIGHT_25_TM=59.174
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=2.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=932
PRIMER_PAIR_26_PENALTY=1.030462
PRIMER_LEFT_26_PENALTY=0.276812
PRIMER_RIGHT_26_PENALTY=0.753650
PRIMER_LEFT_26_SEQUENCE=TTGCTCGAAGCCAAGTCTCT
PRIMER_RIGHT_26_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_26=903,20
PRIMER_RIGHT_26=1561,20
PRIMER_LEFT_26_TM=60.277
PRIMER_RIGHT_26_TM=59.246
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=659
PRIMER_PAIR_27_PENALTY=1.053380
PRIMER_LEFT_27_PENALTY=0.527462
PRIMER_RIGHT_27_PENALTY=0.525918
PRIMER_LEFT_27_SEQUENCE=CCAACGGCTGAGAGTCTTTT
PRIMER_RIGHT_27_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_27=746,20
PRIMER_RIGHT_27=1345,20
PRIMER_LEFT_27_TM=59.473
PRIMER_RIGHT_27_TM=60.526
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.3000
PRIMER_RIGHT_27_END_STABILITY=7.8000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=600
PRIMER_PAIR_28_PENALTY=1.057017
PRIMER_LEFT_28_PENALTY=0.132262
PRIMER_RIGHT_28_PENALTY=0.924755
PRIMER_LEFT_28_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_28_SEQUENCE=TCCGATACATCTCCACGAAA
PRIMER_LEFT_28=900,20
PRIMER_RIGHT_28=1775,20
PRIMER_LEFT_28_TM=60.132
PRIMER_RIGHT_28_TM=59.075
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=9.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=876
PRIMER_PAIR_29_PENALTY=1.063067
PRIMER_LEFT_29_PENALTY=0.309418
PRIMER_RIGHT_29_PENALTY=0.753650
PRIMER_LEFT_29_SEQUENCE=GTTGGGCCTTTTTCTCTCCT
PRIMER_RIGHT_29_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_29=627,20
PRIMER_RIGHT_29=1561,20
PRIMER_LEFT_29_TM=59.691
PRIMER_RIGHT_29_TM=59.246
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=935
PRIMER_PAIR_30_PENALTY=1.063067
PRIMER_LEFT_30_PENALTY=0.309418
PRIMER_RIGHT_30_PENALTY=0.753650
PRIMER_LEFT_30_SEQUENCE=AGTTGGGCCTTTTTCTCTCC
PRIMER_RIGHT_30_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_30=626,20
PRIMER_RIGHT_30=1561,20
PRIMER_LEFT_30_TM=59.691
PRIMER_RIGHT_30_TM=59.246
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=936
PRIMER_PAIR_31_PENALTY=1.067938
PRIMER_LEFT_31_PENALTY=0.179594
PRIMER_RIGHT_31_PENALTY=0.888344
PRIMER_LEFT_31_SEQUENCE=TGGGCCTTTTTCTCTCCTTT
PRIMER_RIGHT_31_SEQUENCE=CATCAAGATGCAAAAATCGG
PRIMER_LEFT_31=629,20
PRIMER_RIGHT_31=1222,20
PRIMER_LEFT_31_TM=60.180
PRIMER_RIGHT_31_TM=59.112
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=9.8000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=594
PRIMER_PAIR_32_PENALTY=1.067938
PRIMER_LEFT_32_PENALTY=0.179594
PRIMER_RIGHT_32_PENALTY=0.888344
PRIMER_LEFT_32_SEQUENCE=TTGGGCCTTTTTCTCTCCTT
PRIMER_RIGHT_32_SEQUENCE=CATCAAGATGCAAAAATCGG
PRIMER_LEFT_32=628,20
PRIMER_RIGHT_32=1222,20
PRIMER_LEFT_32_TM=60.180
PRIMER_RIGHT_32_TM=59.112
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.8000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=595
PRIMER_PAIR_33_PENALTY=1.103085
PRIMER_LEFT_33_PENALTY=0.276812
PRIMER_RIGHT_33_PENALTY=0.826272
PRIMER_LEFT_33_SEQUENCE=TTGCTCGAAGCCAAGTCTCT
PRIMER_RIGHT_33_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_33=903,20
PRIMER_RIGHT_33=1559,20
PRIMER_LEFT_33_TM=60.277
PRIMER_RIGHT_33_TM=59.174
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=657
PRIMER_PAIR_34_PENALTY=1.135690
PRIMER_LEFT_34_PENALTY=0.309418
PRIMER_RIGHT_34_PENALTY=0.826272
PRIMER_LEFT_34_SEQUENCE=GTTGGGCCTTTTTCTCTCCT
PRIMER_RIGHT_34_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_34=627,20
PRIMER_RIGHT_34=1559,20
PRIMER_LEFT_34_TM=59.691
PRIMER_RIGHT_34_TM=59.174
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=2.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=933
PRIMER_PAIR_35_PENALTY=1.135690
PRIMER_LEFT_35_PENALTY=0.309418
PRIMER_RIGHT_35_PENALTY=0.826272
PRIMER_LEFT_35_SEQUENCE=AGTTGGGCCTTTTTCTCTCC
PRIMER_RIGHT_35_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_35=626,20
PRIMER_RIGHT_35=1559,20
PRIMER_LEFT_35_TM=59.691
PRIMER_RIGHT_35_TM=59.174
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=934
PRIMER_PAIR_36_PENALTY=1.183012
PRIMER_LEFT_36_PENALTY=0.657094
PRIMER_RIGHT_36_PENALTY=0.525918
PRIMER_LEFT_36_SEQUENCE=TTTGCTCGAAGCCAAGTCTC
PRIMER_RIGHT_36_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_36=902,20
PRIMER_RIGHT_36=1345,20
PRIMER_LEFT_36_TM=60.657
PRIMER_RIGHT_36_TM=60.526
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.1000
PRIMER_RIGHT_36_END_STABILITY=7.8000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=444
PRIMER_PAIR_37_PENALTY=1.197761
PRIMER_LEFT_37_PENALTY=0.309418
PRIMER_RIGHT_37_PENALTY=0.888344
PRIMER_LEFT_37_SEQUENCE=GTTGGGCCTTTTTCTCTCCT
PRIMER_RIGHT_37_SEQUENCE=CATCAAGATGCAAAAATCGG
PRIMER_LEFT_37=627,20
PRIMER_RIGHT_37=1222,20
PRIMER_LEFT_37_TM=59.691
PRIMER_RIGHT_37_TM=59.112
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.8000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=596
PRIMER_PAIR_38_PENALTY=1.197761
PRIMER_LEFT_38_PENALTY=0.309418
PRIMER_RIGHT_38_PENALTY=0.888344
PRIMER_LEFT_38_SEQUENCE=AGTTGGGCCTTTTTCTCTCC
PRIMER_RIGHT_38_SEQUENCE=CATCAAGATGCAAAAATCGG
PRIMER_LEFT_38=626,20
PRIMER_RIGHT_38=1222,20
PRIMER_LEFT_38_TM=59.691
PRIMER_RIGHT_38_TM=59.112
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=9.8000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=597
PRIMER_PAIR_39_PENALTY=1.201568
PRIMER_LEFT_39_PENALTY=0.276812
PRIMER_RIGHT_39_PENALTY=0.924755
PRIMER_LEFT_39_SEQUENCE=TTGCTCGAAGCCAAGTCTCT
PRIMER_RIGHT_39_SEQUENCE=TCCGATACATCTCCACGAAA
PRIMER_LEFT_39=903,20
PRIMER_RIGHT_39=1775,20
PRIMER_LEFT_39_TM=60.277
PRIMER_RIGHT_39_TM=59.075
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=9.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=873
PRIMER_PAIR_40_PENALTY=1.213299
PRIMER_LEFT_40_PENALTY=0.132262
PRIMER_RIGHT_40_PENALTY=1.081037
PRIMER_LEFT_40_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_40_SEQUENCE=CCGATACATCTCCACGAAAGA
PRIMER_LEFT_40=900,20
PRIMER_RIGHT_40=1774,21
PRIMER_LEFT_40_TM=60.132
PRIMER_RIGHT_40_TM=60.081
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=875
PRIMER_PAIR_41_PENALTY=1.213299
PRIMER_LEFT_41_PENALTY=0.132262
PRIMER_RIGHT_41_PENALTY=1.081037
PRIMER_LEFT_41_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_41_SEQUENCE=TCCGATACATCTCCACGAAAG
PRIMER_LEFT_41=900,20
PRIMER_RIGHT_41=1775,21
PRIMER_LEFT_41_TM=60.132
PRIMER_RIGHT_41_TM=60.081
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=876
PRIMER_PAIR_42_PENALTY=1.220905
PRIMER_LEFT_42_PENALTY=0.132262
PRIMER_RIGHT_42_PENALTY=1.088643
PRIMER_LEFT_42_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_42_SEQUENCE=GAAACAGCCCAATTAAGAGCC
PRIMER_LEFT_42=900,20
PRIMER_RIGHT_42=1830,21
PRIMER_LEFT_42_TM=60.132
PRIMER_RIGHT_42_TM=60.089
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=9.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=931
PRIMER_PAIR_43_PENALTY=1.228122
PRIMER_LEFT_43_PENALTY=0.474473
PRIMER_RIGHT_43_PENALTY=0.753650
PRIMER_LEFT_43_SEQUENCE=CGGTAGTTGGGCCTTTTTCT
PRIMER_RIGHT_43_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_43=622,20
PRIMER_RIGHT_43=1561,20
PRIMER_LEFT_43_TM=60.474
PRIMER_RIGHT_43_TM=59.246
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=940
PRIMER_PAIR_44_PENALTY=1.241604
PRIMER_LEFT_44_PENALTY=0.715687
PRIMER_RIGHT_44_PENALTY=0.525918
PRIMER_LEFT_44_SEQUENCE=GGGCCTTTTTCTCTCCTTTC
PRIMER_RIGHT_44_SEQUENCE=AACACGATCAAACGCATCCT
PRIMER_LEFT_44=630,20
PRIMER_RIGHT_44=1345,20
PRIMER_LEFT_44_TM=59.284
PRIMER_RIGHT_44_TM=60.526
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=7.8000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=716
PRIMER_PAIR_45_PENALTY=1.281111
PRIMER_LEFT_45_PENALTY=0.527462
PRIMER_RIGHT_45_PENALTY=0.753650
PRIMER_LEFT_45_SEQUENCE=CCAACGGCTGAGAGTCTTTT
PRIMER_RIGHT_45_SEQUENCE=AGCCGCATGTAGCTCTTTCT
PRIMER_LEFT_45=746,20
PRIMER_RIGHT_45=1561,20
PRIMER_LEFT_45_TM=59.473
PRIMER_RIGHT_45_TM=59.246
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.3000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=816
PRIMER_PAIR_46_PENALTY=1.300745
PRIMER_LEFT_46_PENALTY=0.474473
PRIMER_RIGHT_46_PENALTY=0.826272
PRIMER_LEFT_46_SEQUENCE=CGGTAGTTGGGCCTTTTTCT
PRIMER_RIGHT_46_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_46=622,20
PRIMER_RIGHT_46=1559,20
PRIMER_LEFT_46_TM=60.474
PRIMER_RIGHT_46_TM=59.174
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=938
PRIMER_PAIR_47_PENALTY=1.342130
PRIMER_LEFT_47_PENALTY=0.132262
PRIMER_RIGHT_47_PENALTY=1.209868
PRIMER_LEFT_47_SEQUENCE=CTTTTGCTCGAAGCCAAGTC
PRIMER_RIGHT_47_SEQUENCE=CAGCAACAAAACAGTCACCAA
PRIMER_LEFT_47=900,20
PRIMER_RIGHT_47=1721,21
PRIMER_LEFT_47_TM=60.132
PRIMER_RIGHT_47_TM=59.790
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=822
PRIMER_PAIR_48_PENALTY=1.353734
PRIMER_LEFT_48_PENALTY=0.527462
PRIMER_RIGHT_48_PENALTY=0.826272
PRIMER_LEFT_48_SEQUENCE=CCAACGGCTGAGAGTCTTTT
PRIMER_RIGHT_48_SEQUENCE=CCGCATGTAGCTCTTTCTCA
PRIMER_LEFT_48=746,20
PRIMER_RIGHT_48=1559,20
PRIMER_LEFT_48_TM=59.473
PRIMER_RIGHT_48_TM=59.174
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=814
PRIMER_PAIR_49_PENALTY=1.357849
PRIMER_LEFT_49_PENALTY=0.276812
PRIMER_RIGHT_49_PENALTY=1.081037
PRIMER_LEFT_49_SEQUENCE=TTGCTCGAAGCCAAGTCTCT
PRIMER_RIGHT_49_SEQUENCE=CCGATACATCTCCACGAAAGA
PRIMER_LEFT_49=903,20
PRIMER_RIGHT_49=1774,21
PRIMER_LEFT_49_TM=60.277
PRIMER_RIGHT_49_TM=60.081
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=872
=
SEQUENCE_ID=MAGIC.105_4_4505792_4506287_kn-0_ler-0_12822_66073_TRUE
SEQUENCE_TARGET=950,595
SEQUENCE_TEMPLATE=GCNCATCACTCNTCTCGGGNNNNNNNNNNNNNNNNNNNNNNNNTNANCTCNACTCTTTNGCTTATACNCNAACTCACCTTTGTAANCNTTGNTTCAGGCAAACANCNNNCTCAATGACATGATTGAGTATTACGAAAGNNTGNTGCTTGNCANGGAANGANGTTATNGCCTNGAAAGANAAGTTTTCTTCTNTTGAGTCCGATCTCNNCTCTTCCNCTGAAGTCANGCNNAAACTNGAAGANCAATTGGACAANCTTTNTTCCGAACTCANNNNNNNNNNNNNGAGTTGNAGGNTCAGTATCANNGGTATGANAANATCNAGGAGGAACTCTCGAATGCTCNNGNCAGACTNTNAGNNNNNNNNNNNNNNNNTTACGACTTGTCCAACCAGCTATCCNAANTGCANGCCANNTACAAAGCAATNGCCAAACNTCGAGATGNTNAGTTANNNNNNNNNNNNNNAAAAGCCAGGAAAGANGTAAANGGTCGCGGGATGGAANTGATTCAANGGGCNATCGTNTTNATCNAAACCGAGAAAACTAGGACGNANNTGGAATCAGANATCAAGGANTATGAAATCAACCTCCTCCTTCTGNGNCAAACCCATGACGAAGATTTTTCNGAAGNNCNNNNNAGGTCCGAATTGNAAGCNGTTNTAACAGAGAANCNAANTCNTCTGGCCGCTCNNCCNTCTTNNTCATTCAACCNTCAANNNNNNNNAGAGTTCTTGACCGAGTCGCCTCCTTTGAGCGAGTCNGGTTTAGATTNNNNNNNNNNNTTTTAAAACCTCAANTTNCGANNNNNNNNNNNNNNANNTTANACTTGCGTTTTCNTGATTCGAGATGATTNTCTCANGNNCCAGTGATCCCNTGGAACCNGAGGTATCNATCATGCCGNTNGANACTCCNANNNNGGTAGCCATCCCNNCAGAAATTCCTCCACCCACGAACACNGAAACAGTGGTNATCGAGGATNATGATGGTTCGNACNCTAGNATTCCNACNNNNNNNNNNNNNNNNNNNNNNNNNGATGAGACTGAGATGGCTGCCGTGGACCCGGTCAATCCTGAGCCAACCCAAACTNNNNANNTNNTGNNAAACTTGAANTTTATTTTTCAAACTANNTTCATCGCCTAAGTNGGNTTNNTATTTTCTGAACAAGTNNTTNNCCTGAGGAGGCTTTTAAACCTNTNNANATTATGGTCCCGAGANGGNNTTTTGCNNTTAANNTGTGGNTTTGCAAANTCCANNTTTTGGAAATAGNTTNNGTTAACCATGTGGNCAAGTTATGANATTCANAGGCTGATNAGACCTATCTCTTNAAANGTGAGTCTGAANTNGNTTTAANTNCGNGAATTCCCCAACTCGGGTTCNNTCAATTCGNCTTATNTNCTGGGCTTGGGNCTTANNNNNTTTTNTTNTATNTAATNAANNNTTTGTCTTTAATGCGACGGTTGCCATTTNNTTNCTNNGNAAATAGNAAAAGCNTCACNCCTNTNAGGCANTANTTNNNNNNNCCTCTATTTCCAAAAATGGTTTAAACCATTNGGACNCGCCANAATTATTANTTTTGTGGTTTNANNAANANCCCTAACTCNCTANANNTAAGAGCACAAACTNTNNNNNATGTNTCAAAANATGATCTCAGAAATTTACTTGGTAAANACTTCAAGNANGGAAAACGAANGANCCTCCACCNTNTGTCCTCTTGACCCNGNNAGCCCACTGNTTCTGTNCGGCGATGGCTTNGAGCCCGCCNNCGTNCTCTTTCCTCACNCNGNNTTTCACTTCCCNNCGAAGGANAANANNNNNNNNTACNATTTNATGGNAAANGTAGGTCTNTTACNCTTTTNCTTANCNNANNNNNTNCNNNNNNNNNNNNNNNNNNNNNNNNCTAGGTNNTCGACAAGCANCNCCGCCTATGCGATGNNNNNNNNNNNNNNNNNNNNNAGAGGNAAANGCTCNAGGCCCGAATTNCCCGNATNGAAAGGAAGGTGAGGGCCATNNAGAGNGATCCCTTCNAATNNAGGAACTTCGACTGTGTGGCGAAGATCCNGCACATGCTNCGGATGTANCTCNGTGCTCGAGGTNNAGTNNNNNNNNNNNNNNNNNNNNTGGTCCAAGTGATNCCGNCTGACNGNNAAGAGNNNGANGACGAGGANGANGACANCNACCCTTGGAAGNGNGTGAAGAATGAACCCAAAGTNGACNTCAANAACNAGGCATCNACCTCNAACCCNGAACCGGCGATGAANGAGCNTGTTCCCGAGCNNCTTCCCTAAATGTCCCNCNANTTCNNATNNNTNNNNNTTTTTTNNATTTTTGTTTGGACTTNNTNTTGGGNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNAAATATTCGTTTCCGAGATACTCTTTGAATGCAGGGNGATNNGGNNGACGTGAATGCTTCCGCNAATCACNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAAGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_6288477_6289040_po-0_ler-0_202439_1332203_TRUE
SEQUENCE_TARGET=950,663
SEQUENCE_TEMPLATE=AGTAGTGTTTGTTTAGTTCCNAGTCCGACNNNNNNNTTAGAAGTGATTTTGGATCCNNAANGTTNTTTTATGANGATTTATAAGAAATTTNANGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATGTANAACTTNTCCGAAATAGGGTTAGAACTCTCNGGGATNCAAATAAGATTGNGANGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTTNGNTTGCCNNTCGNTGTTTGTTTGACNGTNTATGTGTTGTTNGTTAGAGAGTNGCTTTGATTATANNAATTATCCGTCAANGTTTATGTTNTTAAGTNTANGTCAAACTTACAAAGATNNNNNNTANGACNTAANTTATGTATTAAAACAACAATTTACATCAGTTCCATTAGTGATTCTATTAAATAGATGACNATAAACTATGTATAAAAANANTACAANTTTTNNNNNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATGCCAAAACNNTANANAANACNGGAATNGGAAAAANANATCGGACCTCNGCTGATNGACGNAAANAAAANTGACNAGANCCTTAGATCCGGNGTACTCAAGATTNNNAACTTNTNTGNTNCCTCGAAANATCGATGACCACNGNCGGAAGNGAGGCANANATTGNTTGNGACATTNTTTCCTCATATAAAGCTGNTGNTGGTTTACTACTTGAATGATANATTTAACAATCTAAAATTGTGAATGTGAGGTTAAAAGTTTTAANATTATGAGATCGAAAAACTCAAAATTTGATGTGAATGCTCTAATTGTTGTTTANTTGTTTTTCTCTTTTANATNTTTTTTAGTAAGACAATAACTTAGTNACNTACAAAATTTCACGTTTATGGTATGTGCCTTAAAATATAGTTTGTAGGAAGAAAATGTCCACTNTTTTTTTTTNNNNNTTTTTNTTNCCGATTTTNNNNNNNNNNAAACCTCAACAACCAAGGGTCCTACCATACCCTATCTTATTTCTTCAATTGTTTTGCTACCAAAAGAAATCTAGATCTTGATAAAATNAAGCTTGCTATTCTTCAATATATCAAACCCCTTCTCTTTGCTTTCTCACACTGAAATCAAACTCCTTTGGNANTACTACTCTCACTTTTGCTTAAATATTAATATGATCTTNTTCATACATATATTTGTGTCATCATCAGTTTCAAGCTTTTCTCGTTTTCTTATTTNCCTNTCTNTCTCTCCGCAAATAACCATAATTCTCAGCTTTTAATAGTAGTTACTCTGCAGGAGTTGTTTCTCCACTCAAAAGCNNNCATCATTTTCTTAATAAATCTCAATCTCATATGCAAAATTTTCAAACTTTAGCTTATCTTTTGNNNNNNNNNNNNNNCNNTNCATGNNNNNNNNNNNNNTTTCTCATAGTNNNAAAANAANNTCCTTTTGCAATACATGTTTCCATTTAAAGTTTATGTATNTTTTTTGTTCCATGTGTNATAAACTCTTTCCATGACCAGAGAATCTTAATCTCCTTATAGGTAATTAAAGATGNAGAAGAATGGTCACACGGAAAGTGAACTTCAAACTCAAATGGTAATTCATTTGTCTTTTTATATCAAATAGTTAAATCTTNCAAAAAAANTAANANCATCGTCATGTCACAATCAAATTACGTGTCTAAAAGTATTTTGGACCATTCGTTTCCTAAATAGNAATGAAGAATGTGAACTCTTGCTAGCTTTTTACTCTTGAAAAGTATGTCAGTGTCATTTTTATTTCAAANAAAAAAAAAAAAATCTTATTTGTTTCCTTTGTCAACANCTGTTTACTTACAATTTTACTATATTTCACGTTATCTTTGAATTTNTTTTNGTGNAGTTTAACCTCTTGGTGGTTCATATGAGTTTAAACATCAGATCATGCGTTCTAATACTAACTTAATAATGCAATAAGTTTTAAAAAATGTTTTATCACAGAAAGATAAAACAAAATGAAGATATTAAGTATCTGCCTACTTCGAATAAAGTGGAAGATGATATATAATAAGGTCTCTCGAACAAAATGGATGGTAATTAGAGGTCTTGAATATATTAACTCTATATATAAACTAATGNGACTACATTAAAATTATCCACNAAGTGGAGTTTGCATTNGTAAACATATATATTAACTAATGATATAGTTATTAATCTATCAAATTAATATGAAAATCATTAGTGAAAATGACAATAAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_4467909_4467960_sf-2_kn-0_30533_339848_TRUE
SEQUENCE_TARGET=950,151
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_15692206_15692441_ws-0_oy-0_11269_100287_TRUE
SEQUENCE_TARGET=950,335
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_2_6244979_6245650_po-0_wu-0_39476_10027_TRUE
SEQUENCE_TARGET=950,771
SEQUENCE_TEMPLATE=TTGCAACTAACAATTACNTNTTTTTTTTNTCATCACACTATAGAAAAGCTTTNAAAAAGATAGTTGTCTNCTTTCGNAAAAACACGAAACCAATGTAAATTAGAAGATAGTTCGCATATTGATTTGGAAAANTCAGTCCTAAGATTGATGAAGCAAATTAGAGTGAGTGTTTCATGNNNNNNAGNCANTTTTTGGAAGCTTTTCTTTGGATTGAATAAATCCCCACGNGATGATATGTTATCTCTCTGCATGAAAGTAATGAATAAACCTTTTCAACAATCTATTAACAATGCAAAGACTTCTAAGCAAGAAAAGTCGTATAAGTTTTTTCTTTTTGGTANANTTTAGTTTAAAATCGTGTCCGTTGTTTATTTGCCAGATGNCATTTTCTNTTAGATTTAAAATATGTCAGCAATCTATTTAACTTTAANGCGAACGGATAAAATGAGATTTTAGCGGTTAACTTGATCAAAATCTAATAAAAAGTTACAAACTAAAATGAATGATAAATGAAGGTTACATGCATGGAGAAATTTTTTACTTTGTAAATGACTTGCTGCCTAATAAAAATTAGTTATTTTTAATATTTAAAAGNAAACATNATATGTAAGTTAATAAGTATGAATTGTAAGGAAGCAAACATTCTTACGTTTTGAAAAANGAAAACAAAAAGATTCCTACGTAAGTATGTATTNTCTTTGCTTCTCCACAAAGATATTCTTTGTATAGGACACAATTTACTTTAAACACACAATACAATATGTATATNTACTAATCAATGATCGTTATATATATTCTTTGTATAGGACACAATTTACTTTAAACACTTTTCATGCAATACAATATGTATATATACTAATCAATGATCGTTATATATGTCCATCGTACTCAACCTCAAACATCTTAAATAATGACTCTAATAAANCGNTCAAAACCATTTGTACTTCTCNTANGCTTCTNAANTATCTCAAGTTTGCTATTATGGGTCTCACAAGCTTCCNTTGTTTCATCAGNAGATTTTAACAAAGACTTCTTTGTGACATGGTCTCCTACCCACGTTAACACCTCCAATGATGGTCGATCAAGAACCCTNANACTCGATCAAGAATCTGGTATTTTCATATANANGTCCAAATGTCCTNGTTNTATTTTTCNGCATNNAGAACTTATGTTTTGATAATATTAGGAGCTTCTTTTTCTTCCATNCAGACGTTCTTGTTCGGCCAAATAGATATGAAGATCAAACTTATCCNTNGAAGTTCTCAAGGNACCGTGGTTGCTTATTATGTAAGAAACNGTCTCTATCTCTTCATCTTAACGGGTTGATGTGAATGAATCTCATAATTTCCANGATTTATCAGATGTCCTCAGATCAGCCNAACCGGGATGAGATTGACTTTGANTTCCTCGGAAACGTTANTGGGCAGCCTTACATTCTCCAGACAAATGTCTATGCCGANGGANTTGATAACCGCGAAGAAAGGATCCATCTCTGGTTCGACCCANCCAAGGACTTTCACACTTACTCTATCTTGTGGAACATTCACCAAATTGTGTAAGANTNNTCAACCAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGGTGGTTTCAGATTTATGGTGGATCAGATTCCNATAANNTTGTATAGAAACCANGNGGANAAAGGCGTAGCCTACCCAANGTTGCAACCTATGAGTGTACAGGCGAGTCTATGGAANGGTGAGAGCTGGGCTACNNGCGGTGGNCNTGACAAGATTGATTGGTCAAAGGGTCCATTTGTNGCTTNCTTTNGGGATTACAANNTAGACGCTTGTATTTGGANNGGCAATNCAAGTTTTTGTAATGGAGAGAGCACTGAAAACTGGTGGAACAAGAATGAGTTCAGTTCTTTGACAAGAGTGCAAAAGAGATGGTTTAAATGGGTGAGNAAATATCACTTGATTTATGATTATTGCCAAGATTATGGAAGGTTCANTAACAAGCTACCGAAGGAATGTTCTCTTCCTAAATATTNAATTTGCTCAAATCTTNGGTTCNCCTCCTTTTCTNTTAGCCAATAACATTATGCCATGTTANACTGAATTTAAATAAAAATAATTAANTAAAANTAAATCAGAAAAACCAAAAATGGAAAGAAAATNCTTNTATNTATTTAANGTACCTTAACGTCAAGGTTGACATCTTCTCCTNNNNTTNNNNNNTNTAATGTAANTNTTTTAATATGAGCAAATTTGAAAGAANGACNATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGNANGNCACTATGATTGGGGTAANCGAGATNCCGNGGATCTATCATCTCCCACGATCTTAANCGTTCATTTTNTGGCATGAACNATTANATCTCNAAGGGATAATAATTANNNATCCCTTNTGTAAATCACTATCNTGGCTTCATNTATNGGTATTAGTGNACAAGGTTTTGCGGANTGTCATTTGATTCANCTNAGNTAAACTTGCTTTCACANNTAAATAATCANCTCCTAAACAATTTTTTGTTTTGCAATAATCTCAGGNAACAAAGACATGTTTNAAACCATACANTCNTACCATGCANTCAAGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=3.158800
PRIMER_LEFT_0_PENALTY=1.850020
PRIMER_RIGHT_0_PENALTY=1.308781
PRIMER_LEFT_0_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_0_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_0=876,21
PRIMER_RIGHT_0=1793,20
PRIMER_LEFT_0_TM=59.150
PRIMER_RIGHT_0_TM=58.691
PRIMER_LEFT_0_GC_PERCENT=47.619
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=918
PRIMER_PAIR_1_PENALTY=3.339057
PRIMER_LEFT_1_PENALTY=2.030276
PRIMER_RIGHT_1_PENALTY=1.308781
PRIMER_LEFT_1_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_1_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_1=879,22
PRIMER_RIGHT_1=1793,20
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=58.691
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=915
PRIMER_PAIR_2_PENALTY=3.402142
PRIMER_LEFT_2_PENALTY=1.850020
PRIMER_RIGHT_2_PENALTY=1.552122
PRIMER_LEFT_2_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_2_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_2=876,21
PRIMER_RIGHT_2=1790,21
PRIMER_LEFT_2_TM=59.150
PRIMER_RIGHT_2_TM=60.552
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=915
PRIMER_PAIR_3_PENALTY=3.582398
PRIMER_LEFT_3_PENALTY=2.030276
PRIMER_RIGHT_3_PENALTY=1.552122
PRIMER_LEFT_3_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_3_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_3=879,22
PRIMER_RIGHT_3=1790,21
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=60.552
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=912
PRIMER_PAIR_4_PENALTY=3.831224
PRIMER_LEFT_4_PENALTY=2.522443
PRIMER_RIGHT_4_PENALTY=1.308781
PRIMER_LEFT_4_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_4_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_4=875,22
PRIMER_RIGHT_4=1793,20
PRIMER_LEFT_4_TM=59.478
PRIMER_RIGHT_4_TM=58.691
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=919
PRIMER_PAIR_5_PENALTY=3.858872
PRIMER_LEFT_5_PENALTY=2.550092
PRIMER_RIGHT_5_PENALTY=1.308781
PRIMER_LEFT_5_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_5_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_5=876,22
PRIMER_RIGHT_5=1793,20
PRIMER_LEFT_5_TM=60.550
PRIMER_RIGHT_5_TM=58.691
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=918
PRIMER_PAIR_6_PENALTY=4.074565
PRIMER_LEFT_6_PENALTY=2.522443
PRIMER_RIGHT_6_PENALTY=1.552122
PRIMER_LEFT_6_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_6_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_6=875,22
PRIMER_RIGHT_6=1790,21
PRIMER_LEFT_6_TM=59.478
PRIMER_RIGHT_6_TM=60.552
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=916
PRIMER_PAIR_7_PENALTY=4.094179
PRIMER_LEFT_7_PENALTY=2.785399
PRIMER_RIGHT_7_PENALTY=1.308781
PRIMER_LEFT_7_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_7_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_7=878,21
PRIMER_RIGHT_7=1793,20
PRIMER_LEFT_7_TM=58.215
PRIMER_RIGHT_7_TM=58.691
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=916
PRIMER_PAIR_8_PENALTY=4.102214
PRIMER_LEFT_8_PENALTY=2.550092
PRIMER_RIGHT_8_PENALTY=1.552122
PRIMER_LEFT_8_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_8_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_8=876,22
PRIMER_RIGHT_8=1790,21
PRIMER_LEFT_8_TM=60.550
PRIMER_RIGHT_8_TM=60.552
PRIMER_LEFT_8_GC_PERCENT=45.455
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=915
PRIMER_PAIR_9_PENALTY=4.207842
PRIMER_LEFT_9_PENALTY=2.899062
PRIMER_RIGHT_9_PENALTY=1.308781
PRIMER_LEFT_9_SEQUENCE=TCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_9_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_9=878,22
PRIMER_RIGHT_9=1793,20
PRIMER_LEFT_9_TM=59.101
PRIMER_RIGHT_9_TM=58.691
PRIMER_LEFT_9_GC_PERCENT=45.455
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=916
PRIMER_PAIR_10_PENALTY=4.207842
PRIMER_LEFT_10_PENALTY=2.899062
PRIMER_RIGHT_10_PENALTY=1.308781
PRIMER_LEFT_10_SEQUENCE=GTCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_10_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_10=877,22
PRIMER_RIGHT_10=1793,20
PRIMER_LEFT_10_TM=59.101
PRIMER_RIGHT_10_TM=58.691
PRIMER_LEFT_10_GC_PERCENT=45.455
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=917
PRIMER_PAIR_11_PENALTY=4.337521
PRIMER_LEFT_11_PENALTY=2.785399
PRIMER_RIGHT_11_PENALTY=1.552122
PRIMER_LEFT_11_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_11_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_11=878,21
PRIMER_RIGHT_11=1790,21
PRIMER_LEFT_11_TM=58.215
PRIMER_RIGHT_11_TM=60.552
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=913
PRIMER_PAIR_12_PENALTY=4.387949
PRIMER_LEFT_12_PENALTY=3.079168
PRIMER_RIGHT_12_PENALTY=1.308781
PRIMER_LEFT_12_SEQUENCE=GTCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_12_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_12=877,23
PRIMER_RIGHT_12=1793,20
PRIMER_LEFT_12_TM=59.921
PRIMER_RIGHT_12_TM=58.691
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=917
PRIMER_PAIR_13_PENALTY=4.451184
PRIMER_LEFT_13_PENALTY=2.899062
PRIMER_RIGHT_13_PENALTY=1.552122
PRIMER_LEFT_13_SEQUENCE=TCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_13_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_13=878,22
PRIMER_RIGHT_13=1790,21
PRIMER_LEFT_13_TM=59.101
PRIMER_RIGHT_13_TM=60.552
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=913
PRIMER_PAIR_14_PENALTY=4.451184
PRIMER_LEFT_14_PENALTY=2.899062
PRIMER_RIGHT_14_PENALTY=1.552122
PRIMER_LEFT_14_SEQUENCE=GTCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_14_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_14=877,22
PRIMER_RIGHT_14=1790,21
PRIMER_LEFT_14_TM=59.101
PRIMER_RIGHT_14_TM=60.552
PRIMER_LEFT_14_GC_PERCENT=45.455
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=914
PRIMER_PAIR_15_PENALTY=4.631290
PRIMER_LEFT_15_PENALTY=3.079168
PRIMER_RIGHT_15_PENALTY=1.552122
PRIMER_LEFT_15_SEQUENCE=GTCCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_15_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_15=877,23
PRIMER_RIGHT_15=1790,21
PRIMER_LEFT_15_TM=59.921
PRIMER_RIGHT_15_TM=60.552
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=914
PRIMER_PAIR_16_PENALTY=4.653731
PRIMER_LEFT_16_PENALTY=3.344951
PRIMER_RIGHT_16_PENALTY=1.308781
PRIMER_LEFT_16_SEQUENCE=GTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_16_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_16=877,21
PRIMER_RIGHT_16=1793,20
PRIMER_LEFT_16_TM=57.655
PRIMER_RIGHT_16_TM=58.691
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=917
PRIMER_PAIR_17_PENALTY=4.654933
PRIMER_LEFT_17_PENALTY=3.346153
PRIMER_RIGHT_17_PENALTY=1.308781
PRIMER_LEFT_17_SEQUENCE=TCCATCGTACTCAACCTCAA
PRIMER_RIGHT_17_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_17=878,20
PRIMER_RIGHT_17=1793,20
PRIMER_LEFT_17_TM=56.654
PRIMER_RIGHT_17_TM=58.691
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=916
PRIMER_PAIR_18_PENALTY=4.800991
PRIMER_LEFT_18_PENALTY=3.492211
PRIMER_RIGHT_18_PENALTY=1.308781
PRIMER_LEFT_18_SEQUENCE=TATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_18_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_18=874,23
PRIMER_RIGHT_18=1793,20
PRIMER_LEFT_18_TM=59.508
PRIMER_RIGHT_18_TM=58.691
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=920
PRIMER_PAIR_19_PENALTY=4.890326
PRIMER_LEFT_19_PENALTY=1.850020
PRIMER_RIGHT_19_PENALTY=3.040306
PRIMER_LEFT_19_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_19_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_19=876,21
PRIMER_RIGHT_19=1789,21
PRIMER_LEFT_19_TM=59.150
PRIMER_RIGHT_19_TM=57.960
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=914
PRIMER_PAIR_20_PENALTY=4.897073
PRIMER_LEFT_20_PENALTY=3.344951
PRIMER_RIGHT_20_PENALTY=1.552122
PRIMER_LEFT_20_SEQUENCE=GTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_20_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_20=877,21
PRIMER_RIGHT_20=1790,21
PRIMER_LEFT_20_TM=57.655
PRIMER_RIGHT_20_TM=60.552
PRIMER_LEFT_20_GC_PERCENT=47.619
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=914
PRIMER_PAIR_21_PENALTY=4.898275
PRIMER_LEFT_21_PENALTY=3.346153
PRIMER_RIGHT_21_PENALTY=1.552122
PRIMER_LEFT_21_SEQUENCE=TCCATCGTACTCAACCTCAA
PRIMER_RIGHT_21_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_21=878,20
PRIMER_RIGHT_21=1790,21
PRIMER_LEFT_21_TM=56.654
PRIMER_RIGHT_21_TM=60.552
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.9000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=913
PRIMER_PAIR_22_PENALTY=4.997908
PRIMER_LEFT_22_PENALTY=1.850020
PRIMER_RIGHT_22_PENALTY=3.147888
PRIMER_LEFT_22_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_22_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_22=876,21
PRIMER_RIGHT_22=1789,20
PRIMER_LEFT_22_TM=59.150
PRIMER_RIGHT_22_TM=56.852
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=914
PRIMER_PAIR_23_PENALTY=5.044333
PRIMER_LEFT_23_PENALTY=3.492211
PRIMER_RIGHT_23_PENALTY=1.552122
PRIMER_LEFT_23_SEQUENCE=TATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_23_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_23=874,23
PRIMER_RIGHT_23=1790,21
PRIMER_LEFT_23_TM=59.508
PRIMER_RIGHT_23_TM=60.552
PRIMER_LEFT_23_GC_PERCENT=43.478
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.9000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=917
PRIMER_PAIR_24_PENALTY=5.070582
PRIMER_LEFT_24_PENALTY=2.030276
PRIMER_RIGHT_24_PENALTY=3.040306
PRIMER_LEFT_24_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_24_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_24=879,22
PRIMER_RIGHT_24=1789,21
PRIMER_LEFT_24_TM=60.030
PRIMER_RIGHT_24_TM=57.960
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=911
PRIMER_PAIR_25_PENALTY=5.096035
PRIMER_LEFT_25_PENALTY=3.787254
PRIMER_RIGHT_25_PENALTY=1.308781
PRIMER_LEFT_25_SEQUENCE=ATGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_25_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_25=875,23
PRIMER_RIGHT_25=1793,20
PRIMER_LEFT_25_TM=60.787
PRIMER_RIGHT_25_TM=58.691
PRIMER_LEFT_25_GC_PERCENT=43.478
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=919
PRIMER_PAIR_26_PENALTY=5.113770
PRIMER_LEFT_26_PENALTY=3.804989
PRIMER_RIGHT_26_PENALTY=1.308781
PRIMER_LEFT_26_SEQUENCE=CCATCGTACTCAACCTCAAA
PRIMER_RIGHT_26_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_26=879,20
PRIMER_RIGHT_26=1793,20
PRIMER_LEFT_26_TM=56.195
PRIMER_RIGHT_26_TM=58.691
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=6.9000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=915
PRIMER_PAIR_27_PENALTY=5.118545
PRIMER_LEFT_27_PENALTY=3.809765
PRIMER_RIGHT_27_PENALTY=1.308781
PRIMER_LEFT_27_SEQUENCE=CCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_27_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_27=879,21
PRIMER_RIGHT_27=1793,20
PRIMER_LEFT_27_TM=57.190
PRIMER_RIGHT_27_TM=58.691
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=915
PRIMER_PAIR_28_PENALTY=5.178165
PRIMER_LEFT_28_PENALTY=2.030276
PRIMER_RIGHT_28_PENALTY=3.147888
PRIMER_LEFT_28_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_28_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_28=879,22
PRIMER_RIGHT_28=1789,20
PRIMER_LEFT_28_TM=60.030
PRIMER_RIGHT_28_TM=56.852
PRIMER_LEFT_28_GC_PERCENT=45.455
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=911
PRIMER_PAIR_29_PENALTY=5.274765
PRIMER_LEFT_29_PENALTY=3.965985
PRIMER_RIGHT_29_PENALTY=1.308781
PRIMER_LEFT_29_SEQUENCE=GTCCATCGTACTCAACCTCA
PRIMER_RIGHT_29_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_29=877,20
PRIMER_RIGHT_29=1793,20
PRIMER_LEFT_29_TM=56.034
PRIMER_RIGHT_29_TM=58.691
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.9000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=917
PRIMER_PAIR_30_PENALTY=5.274765
PRIMER_LEFT_30_PENALTY=3.965985
PRIMER_RIGHT_30_PENALTY=1.308781
PRIMER_LEFT_30_SEQUENCE=TGTCCATCGTACTCAACCTC
PRIMER_RIGHT_30_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_30=876,20
PRIMER_RIGHT_30=1793,20
PRIMER_LEFT_30_TM=56.034
PRIMER_RIGHT_30_TM=58.691
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=6.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=918
PRIMER_PAIR_31_PENALTY=5.281379
PRIMER_LEFT_31_PENALTY=1.850020
PRIMER_RIGHT_31_PENALTY=3.431360
PRIMER_LEFT_31_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_31_SEQUENCE=GGACCCTTTGACCAATCAATCT
PRIMER_LEFT_31=876,21
PRIMER_RIGHT_31=1790,22
PRIMER_LEFT_31_TM=59.150
PRIMER_RIGHT_31_TM=61.431
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=915
PRIMER_PAIR_32_PENALTY=5.339376
PRIMER_LEFT_32_PENALTY=3.787254
PRIMER_RIGHT_32_PENALTY=1.552122
PRIMER_LEFT_32_SEQUENCE=ATGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_32_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_32=875,23
PRIMER_RIGHT_32=1790,21
PRIMER_LEFT_32_TM=60.787
PRIMER_RIGHT_32_TM=60.552
PRIMER_LEFT_32_GC_PERCENT=43.478
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=916
PRIMER_PAIR_33_PENALTY=5.357111
PRIMER_LEFT_33_PENALTY=3.804989
PRIMER_RIGHT_33_PENALTY=1.552122
PRIMER_LEFT_33_SEQUENCE=CCATCGTACTCAACCTCAAA
PRIMER_RIGHT_33_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_33=879,20
PRIMER_RIGHT_33=1790,21
PRIMER_LEFT_33_TM=56.195
PRIMER_RIGHT_33_TM=60.552
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=912
PRIMER_PAIR_34_PENALTY=5.361887
PRIMER_LEFT_34_PENALTY=3.809765
PRIMER_RIGHT_34_PENALTY=1.552122
PRIMER_LEFT_34_SEQUENCE=CCATCGTACTCAACCTCAAAC
PRIMER_RIGHT_34_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_34=879,21
PRIMER_RIGHT_34=1790,21
PRIMER_LEFT_34_TM=57.190
PRIMER_RIGHT_34_TM=60.552
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=912
PRIMER_PAIR_35_PENALTY=5.461636
PRIMER_LEFT_35_PENALTY=2.030276
PRIMER_RIGHT_35_PENALTY=3.431360
PRIMER_LEFT_35_SEQUENCE=CCATCGTACTCAACCTCAAACA
PRIMER_RIGHT_35_SEQUENCE=GGACCCTTTGACCAATCAATCT
PRIMER_LEFT_35=879,22
PRIMER_RIGHT_35=1790,22
PRIMER_LEFT_35_TM=60.030
PRIMER_RIGHT_35_TM=61.431
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=6.6000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=912
PRIMER_PAIR_36_PENALTY=5.518107
PRIMER_LEFT_36_PENALTY=3.965985
PRIMER_RIGHT_36_PENALTY=1.552122
PRIMER_LEFT_36_SEQUENCE=GTCCATCGTACTCAACCTCA
PRIMER_RIGHT_36_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_36=877,20
PRIMER_RIGHT_36=1790,21
PRIMER_LEFT_36_TM=56.034
PRIMER_RIGHT_36_TM=60.552
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=914
PRIMER_PAIR_37_PENALTY=5.518107
PRIMER_LEFT_37_PENALTY=3.965985
PRIMER_RIGHT_37_PENALTY=1.552122
PRIMER_LEFT_37_SEQUENCE=TGTCCATCGTACTCAACCTC
PRIMER_RIGHT_37_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_37=876,20
PRIMER_RIGHT_37=1790,21
PRIMER_LEFT_37_TM=56.034
PRIMER_RIGHT_37_TM=60.552
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.6000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=915
PRIMER_PAIR_38_PENALTY=5.519666
PRIMER_LEFT_38_PENALTY=4.210886
PRIMER_RIGHT_38_PENALTY=1.308781
PRIMER_LEFT_38_SEQUENCE=ATATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_38_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_38=873,24
PRIMER_RIGHT_38=1793,20
PRIMER_LEFT_38_TM=59.789
PRIMER_RIGHT_38_TM=58.691
PRIMER_LEFT_38_GC_PERCENT=41.667
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=921
PRIMER_PAIR_39_PENALTY=5.562749
PRIMER_LEFT_39_PENALTY=2.522443
PRIMER_RIGHT_39_PENALTY=3.040306
PRIMER_LEFT_39_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_39_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_39=875,22
PRIMER_RIGHT_39=1789,21
PRIMER_LEFT_39_TM=59.478
PRIMER_RIGHT_39_TM=57.960
PRIMER_LEFT_39_GC_PERCENT=45.455
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=915
PRIMER_PAIR_40_PENALTY=5.590398
PRIMER_LEFT_40_PENALTY=2.550092
PRIMER_RIGHT_40_PENALTY=3.040306
PRIMER_LEFT_40_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_40_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_40=876,22
PRIMER_RIGHT_40=1789,21
PRIMER_LEFT_40_TM=60.550
PRIMER_RIGHT_40_TM=57.960
PRIMER_LEFT_40_GC_PERCENT=45.455
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=914
PRIMER_PAIR_41_PENALTY=5.668280
PRIMER_LEFT_41_PENALTY=4.359500
PRIMER_RIGHT_41_PENALTY=1.308781
PRIMER_LEFT_41_SEQUENCE=CATCGTACTCAACCTCAAACATC
PRIMER_RIGHT_41_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_41=880,23
PRIMER_RIGHT_41=1793,20
PRIMER_LEFT_41_TM=58.641
PRIMER_RIGHT_41_TM=58.691
PRIMER_LEFT_41_GC_PERCENT=43.478
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.3000
PRIMER_RIGHT_41_END_STABILITY=6.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=914
PRIMER_PAIR_42_PENALTY=5.670331
PRIMER_LEFT_42_PENALTY=2.522443
PRIMER_RIGHT_42_PENALTY=3.147888
PRIMER_LEFT_42_SEQUENCE=ATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_42_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_42=875,22
PRIMER_RIGHT_42=1789,20
PRIMER_LEFT_42_TM=59.478
PRIMER_RIGHT_42_TM=56.852
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=6.9000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=915
PRIMER_PAIR_43_PENALTY=5.697980
PRIMER_LEFT_43_PENALTY=2.550092
PRIMER_RIGHT_43_PENALTY=3.147888
PRIMER_LEFT_43_SEQUENCE=TGTCCATCGTACTCAACCTCAA
PRIMER_RIGHT_43_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_43=876,22
PRIMER_RIGHT_43=1789,20
PRIMER_LEFT_43_TM=60.550
PRIMER_RIGHT_43_TM=56.852
PRIMER_LEFT_43_GC_PERCENT=45.455
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=914
PRIMER_PAIR_44_PENALTY=5.759885
PRIMER_LEFT_44_PENALTY=4.451104
PRIMER_RIGHT_44_PENALTY=1.308781
PRIMER_LEFT_44_SEQUENCE=ATGTCCATCGTACTCAACCTC
PRIMER_RIGHT_44_SEQUENCE=AATGGACCCTTTGACCAATC
PRIMER_LEFT_44=875,21
PRIMER_RIGHT_44=1793,20
PRIMER_LEFT_44_TM=56.549
PRIMER_RIGHT_44_TM=58.691
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.6000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=919
PRIMER_PAIR_45_PENALTY=5.763008
PRIMER_LEFT_45_PENALTY=4.210886
PRIMER_RIGHT_45_PENALTY=1.552122
PRIMER_LEFT_45_SEQUENCE=ATATGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_45_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_45=873,24
PRIMER_RIGHT_45=1790,21
PRIMER_LEFT_45_TM=59.789
PRIMER_RIGHT_45_TM=60.552
PRIMER_LEFT_45_GC_PERCENT=41.667
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=918
PRIMER_PAIR_46_PENALTY=5.825704
PRIMER_LEFT_46_PENALTY=2.785399
PRIMER_RIGHT_46_PENALTY=3.040306
PRIMER_LEFT_46_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_46_SEQUENCE=GACCCTTTGACCAATCAATCT
PRIMER_LEFT_46=878,21
PRIMER_RIGHT_46=1789,21
PRIMER_LEFT_46_TM=58.215
PRIMER_RIGHT_46_TM=57.960
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=6.6000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=912
PRIMER_PAIR_47_PENALTY=5.858729
PRIMER_LEFT_47_PENALTY=1.850020
PRIMER_RIGHT_47_PENALTY=4.008710
PRIMER_LEFT_47_SEQUENCE=TGTCCATCGTACTCAACCTCA
PRIMER_RIGHT_47_SEQUENCE=ACCCTTTGACCAATCAATCT
PRIMER_LEFT_47=876,21
PRIMER_RIGHT_47=1788,20
PRIMER_LEFT_47_TM=59.150
PRIMER_RIGHT_47_TM=55.991
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=913
PRIMER_PAIR_48_PENALTY=5.911622
PRIMER_LEFT_48_PENALTY=4.359500
PRIMER_RIGHT_48_PENALTY=1.552122
PRIMER_LEFT_48_SEQUENCE=CATCGTACTCAACCTCAAACATC
PRIMER_RIGHT_48_SEQUENCE=GGACCCTTTGACCAATCAATC
PRIMER_LEFT_48=880,23
PRIMER_RIGHT_48=1790,21
PRIMER_LEFT_48_TM=58.641
PRIMER_RIGHT_48_TM=60.552
PRIMER_LEFT_48_GC_PERCENT=43.478
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.3000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=911
PRIMER_PAIR_49_PENALTY=5.933287
PRIMER_LEFT_49_PENALTY=2.785399
PRIMER_RIGHT_49_PENALTY=3.147888
PRIMER_LEFT_49_SEQUENCE=TCCATCGTACTCAACCTCAAA
PRIMER_RIGHT_49_SEQUENCE=GACCCTTTGACCAATCAATC
PRIMER_LEFT_49=878,21
PRIMER_RIGHT_49=1789,20
PRIMER_LEFT_49_TM=58.215
PRIMER_RIGHT_49_TM=56.852
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=912
=
SEQUENCE_ID=MAGIC.105_4_12118767_12118940_ler-0_wu-0_50034_8097_TRUE
SEQUENCE_TARGET=950,273
SEQUENCE_TEMPLATE=TCATGATTACTCCGGAAGAAAAATCCAGGTGGTTGAGGTACAGCGAGGGCGATCGAGCTAGTAGTAAGCATTTGGACGATCGCTGACATGGTTGGACGATTTTCAGTATCTTCTTGGACACATAATAAGGCAATATGGATGCATCTAATTATTTCGTTTCTTTGATAACTATCTCGAAAGGAAGAATCCACGAGGTCTAGNGGTGACCCGTCACTCCATAGCCTCCAAGTCTATTNAACANTTTAAGACTNATATGTTATGANTGTTTAGTAGAGCAAAAAAGTNTAAGTGTTTATTATACTCACATATGTAACCAAGTTTCCAAAACTANCATCCATCTGATAGAGGCTGCTATTTTTCCTCCCGNTTATAATCTCAAGAACTAAGACACCGAAGCTATAGACATCAGATTTCATTGANAATTGGCCNTACATNGCATACTCGGGAGACATGTAACCACTGCCACNATACNTANATGTTTGAGTTAATAGAGNATTTTTATATTTAATAGATGGCAAGANCAAAACTTACTAGGTTCCAACNACTNTTCTCGTATGGGCTTCTGTTTGGTCNATTTCNAAAATCCTCGCCATTCCAAAATCTGCAACTTTCGGGTTCATATCAGCATCCAAAAGGATGTTACCNGCTTTTAGGTCTCGATGTATGATTGTNAGTCGTGAATCTTGATGGAGATACANAATTCNTCTAGCAATNCCTCCTATGATCTTGTACCGTGTAGTCCAATCCAGCTGGCTCTGCATTCTAGAATCTNCACATNCATATAGTTAGAATACCTAAANCATGAGTTCCAAAAGGAAATATCTTTCCTAAGCTAAANCTTACCAAACAAGAAGTAATCAAGGCTTTTATTAGACACAAACTCGTAGACAAGTATTTTCTCTTCTCTCTCCAAACAAAATCCAAGAAGCTTGACCAGATTTCTGTGCTGAAGCTTNGCCACAACAACAACTTCGTTCTTGAACTCTTTCTCACCTTGTCCNGATGTTTTNGATAGTCTCTTCACGGCAACTTGTANTCCATTAGGAAGTNTGCCCTAAAAAGNTTGTGAATTAAACAGAGCCNTCCCCTTATTTGATCCTCTTATNCAAGTAACAAGGAAANTCAATGAACNGGTNGTAGTACCTTGTAAACTTGTCCAAATCCACCTTGACCNAGTTTGTTACANATCGAAAACTTATCTGTNGCAGCTTCAATAACNTTNAAATCAAACTGCANTGANCCTGCAGTTGTNATGTCATCCNCTTCTGCAAAATATTTAAGAATTCAAATCCATAAAAGTTTAAGCAACNGNCNCATAGATTTTGGAATCTTTACCATCATTTGCANCTGGTGTATCATAAGTCTTCTTTGCTNTCTTTGNTGCATGGAAGCNAAAAACAGCNACACAAATAAGAACNGAGATAGCGACTGGCACANCAATTGCTATGATTATCACTGTAGAATTTTTGCCTTTTCCTGCATAAGTCTTAGATTAGACATGAANTNAAACCAAACTAACTATTAGGTAAATAAATAAAACAGAGCTCTAACCAGGCGGAAAGGAAGNAGTTGAAACCAGTGGNAGNGGAGGAGCCGAAACCGGCGGAGGAAGAACAGTTCCCTCTATAGTTTCCNTGTAAAANGNGTAAACCTCGTACCTTGAGTTACAACTTGGGACAAGAAATCTTCCTCCAACTTTGTTNAAATATAATTCTTTGATGGATTGTTGAAGACACNTTACNCAATCTTCACTAGTCAGATCAGGAATGCACTGAACCGATGCATAAAGNGACNGAGACGAACNGANATCCGACTTCTNAACCGCGAATTTCTTGCTTGTACTATTAGCCGCTTCCTCTGCAGACTTTTTCATCAGAGANANCACTGCATCATTAAANCGGTCAGATTGGTCCGNCGNAACNTTCTGAGTGTTCCAAGTGATTATTGTAGTTGTGGTTACANTATCCATGAATATATTCCTGTCTGAGTATCCAAGCATGCACTCATCATACTGAATCAAGAACTCTTTCCCACCTGGACATCGACTCNGAGTGTCTTTAGNAGCAAAGATGACGCACTCACGGCAAGNTTCNGGCGAGAGATCTCCTTTNCAGAGATAAAGTCCGAACACCATGNNGGAGTTTTGACCCTTTGTNAGACTATGAGATCCCAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.655102
PRIMER_LEFT_0_PENALTY=0.006945
PRIMER_RIGHT_0_PENALTY=1.648157
PRIMER_LEFT_0_SEQUENCE=CGTATGGGCTTCTGTTTGGT
PRIMER_RIGHT_0_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_0=551,20
PRIMER_RIGHT_0=1486,21
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=59.352
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=10.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=936
PRIMER_PAIR_1_PENALTY=1.731877
PRIMER_LEFT_1_PENALTY=0.636033
PRIMER_RIGHT_1_PENALTY=1.095845
PRIMER_LEFT_1_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_1_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_1=599,20
PRIMER_RIGHT_1=1565,20
PRIMER_LEFT_1_TM=60.636
PRIMER_RIGHT_1_TM=61.096
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=967
PRIMER_PAIR_2_PENALTY=1.921643
PRIMER_LEFT_2_PENALTY=0.825798
PRIMER_RIGHT_2_PENALTY=1.095845
PRIMER_LEFT_2_SEQUENCE=AATCTGCAACTTTCGGGTTC
PRIMER_RIGHT_2_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_2=598,20
PRIMER_RIGHT_2=1565,20
PRIMER_LEFT_2_TM=59.174
PRIMER_RIGHT_2_TM=61.096
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=968
PRIMER_PAIR_3_PENALTY=1.962346
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=1.955401
PRIMER_LEFT_3_SEQUENCE=CGTATGGGCTTCTGTTTGGT
PRIMER_RIGHT_3_SEQUENCE=CTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_3=551,20
PRIMER_RIGHT_3=1484,20
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=58.045
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=934
PRIMER_PAIR_4_PENALTY=2.035742
PRIMER_LEFT_4_PENALTY=0.006945
PRIMER_RIGHT_4_PENALTY=2.028797
PRIMER_LEFT_4_SEQUENCE=CGTATGGGCTTCTGTTTGGT
PRIMER_RIGHT_4_SEQUENCE=GACTTATGCAGGAAAAGGCA
PRIMER_LEFT_4=551,20
PRIMER_RIGHT_4=1486,20
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=57.971
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=936
PRIMER_PAIR_5_PENALTY=2.180947
PRIMER_LEFT_5_PENALTY=1.085102
PRIMER_RIGHT_5_PENALTY=1.095845
PRIMER_LEFT_5_SEQUENCE=TTTCGGGTTCATATCAGCATC
PRIMER_RIGHT_5_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_5=608,21
PRIMER_RIGHT_5=1565,20
PRIMER_LEFT_5_TM=59.915
PRIMER_RIGHT_5_TM=61.096
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=5.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=958
PRIMER_PAIR_6_PENALTY=2.253479
PRIMER_LEFT_6_PENALTY=1.157635
PRIMER_RIGHT_6_PENALTY=1.095845
PRIMER_LEFT_6_SEQUENCE=ATCCTCGCCATTCCAAAATC
PRIMER_RIGHT_6_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_6=582,20
PRIMER_RIGHT_6=1565,20
PRIMER_LEFT_6_TM=61.158
PRIMER_RIGHT_6_TM=61.096
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=984
PRIMER_PAIR_7_PENALTY=2.256334
PRIMER_LEFT_7_PENALTY=0.636033
PRIMER_RIGHT_7_PENALTY=1.620302
PRIMER_LEFT_7_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_7_SEQUENCE=TCCTTTCCGCCTGGTTAGAG
PRIMER_LEFT_7=599,20
PRIMER_RIGHT_7=1563,20
PRIMER_LEFT_7_TM=60.636
PRIMER_RIGHT_7_TM=61.620
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=5.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=965
PRIMER_PAIR_8_PENALTY=2.263447
PRIMER_LEFT_8_PENALTY=0.006945
PRIMER_RIGHT_8_PENALTY=2.256503
PRIMER_LEFT_8_SEQUENCE=CGTATGGGCTTCTGTTTGGT
PRIMER_RIGHT_8_SEQUENCE=AGACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_8=551,20
PRIMER_RIGHT_8=1487,22
PRIMER_LEFT_8_TM=59.993
PRIMER_RIGHT_8_TM=60.257
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=937
PRIMER_PAIR_9_PENALTY=2.284190
PRIMER_LEFT_9_PENALTY=0.636033
PRIMER_RIGHT_9_PENALTY=1.648157
PRIMER_LEFT_9_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_9_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_9=599,20
PRIMER_RIGHT_9=1486,21
PRIMER_LEFT_9_TM=60.636
PRIMER_RIGHT_9_TM=59.352
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=10.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=888
PRIMER_PAIR_10_PENALTY=2.319624
PRIMER_LEFT_10_PENALTY=1.223779
PRIMER_RIGHT_10_PENALTY=1.095845
PRIMER_LEFT_10_SEQUENCE=GGGTTCATATCAGCATCCAAA
PRIMER_RIGHT_10_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_10=612,21
PRIMER_RIGHT_10=1565,20
PRIMER_LEFT_10_TM=59.776
PRIMER_RIGHT_10_TM=61.096
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.8000
PRIMER_RIGHT_10_END_STABILITY=5.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=954
PRIMER_PAIR_11_PENALTY=2.446099
PRIMER_LEFT_11_PENALTY=0.825798
PRIMER_RIGHT_11_PENALTY=1.620302
PRIMER_LEFT_11_SEQUENCE=AATCTGCAACTTTCGGGTTC
PRIMER_RIGHT_11_SEQUENCE=TCCTTTCCGCCTGGTTAGAG
PRIMER_LEFT_11=598,20
PRIMER_RIGHT_11=1563,20
PRIMER_LEFT_11_TM=59.174
PRIMER_RIGHT_11_TM=61.620
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=5.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=966
PRIMER_PAIR_12_PENALTY=2.473955
PRIMER_LEFT_12_PENALTY=0.825798
PRIMER_RIGHT_12_PENALTY=1.648157
PRIMER_LEFT_12_SEQUENCE=AATCTGCAACTTTCGGGTTC
PRIMER_RIGHT_12_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_12=598,20
PRIMER_RIGHT_12=1486,21
PRIMER_LEFT_12_TM=59.174
PRIMER_RIGHT_12_TM=59.352
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=10.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=889
PRIMER_PAIR_13_PENALTY=2.491850
PRIMER_LEFT_13_PENALTY=0.006945
PRIMER_RIGHT_13_PENALTY=2.484905
PRIMER_LEFT_13_SEQUENCE=CGTATGGGCTTCTGTTTGGT
PRIMER_RIGHT_13_SEQUENCE=ACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_13=551,20
PRIMER_RIGHT_13=1485,20
PRIMER_LEFT_13_TM=59.993
PRIMER_RIGHT_13_TM=57.515
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=10.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=935
PRIMER_PAIR_14_PENALTY=2.574385
PRIMER_LEFT_14_PENALTY=1.478540
PRIMER_RIGHT_14_PENALTY=1.095845
PRIMER_LEFT_14_SEQUENCE=TTCGGGTTCATATCAGCATC
PRIMER_RIGHT_14_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_14=609,20
PRIMER_RIGHT_14=1565,20
PRIMER_LEFT_14_TM=58.521
PRIMER_RIGHT_14_TM=61.096
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=957
PRIMER_PAIR_15_PENALTY=2.591434
PRIMER_LEFT_15_PENALTY=0.636033
PRIMER_RIGHT_15_PENALTY=1.955401
PRIMER_LEFT_15_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_15_SEQUENCE=CTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_15=599,20
PRIMER_RIGHT_15=1484,20
PRIMER_LEFT_15_TM=60.636
PRIMER_RIGHT_15_TM=58.045
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.8000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=886
PRIMER_PAIR_16_PENALTY=2.602435
PRIMER_LEFT_16_PENALTY=0.006945
PRIMER_RIGHT_16_PENALTY=2.595491
PRIMER_LEFT_16_SEQUENCE=CGTATGGGCTTCTGTTTGGT
PRIMER_RIGHT_16_SEQUENCE=GACTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_16=551,20
PRIMER_RIGHT_16=1486,22
PRIMER_LEFT_16_TM=59.993
PRIMER_RIGHT_16_TM=60.595
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.8000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=936
PRIMER_PAIR_17_PENALTY=2.608039
PRIMER_LEFT_17_PENALTY=0.636033
PRIMER_RIGHT_17_PENALTY=1.972007
PRIMER_LEFT_17_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_17_SEQUENCE=TTCCTTTCCGCCTGGTTAGA
PRIMER_LEFT_17=599,20
PRIMER_RIGHT_17=1564,20
PRIMER_LEFT_17_TM=60.636
PRIMER_RIGHT_17_TM=61.972
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=966
PRIMER_PAIR_18_PENALTY=2.664830
PRIMER_LEFT_18_PENALTY=0.636033
PRIMER_RIGHT_18_PENALTY=2.028797
PRIMER_LEFT_18_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_18_SEQUENCE=GACTTATGCAGGAAAAGGCA
PRIMER_LEFT_18=599,20
PRIMER_RIGHT_18=1486,20
PRIMER_LEFT_18_TM=60.636
PRIMER_RIGHT_18_TM=57.971
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=888
PRIMER_PAIR_19_PENALTY=2.668862
PRIMER_LEFT_19_PENALTY=1.020705
PRIMER_RIGHT_19_PENALTY=1.648157
PRIMER_LEFT_19_SEQUENCE=TCGTATGGGCTTCTGTTTGG
PRIMER_RIGHT_19_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_19=550,20
PRIMER_RIGHT_19=1486,21
PRIMER_LEFT_19_TM=61.021
PRIMER_RIGHT_19_TM=59.352
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.8000
PRIMER_RIGHT_19_END_STABILITY=10.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=937
PRIMER_PAIR_20_PENALTY=2.705404
PRIMER_LEFT_20_PENALTY=1.085102
PRIMER_RIGHT_20_PENALTY=1.620302
PRIMER_LEFT_20_SEQUENCE=TTTCGGGTTCATATCAGCATC
PRIMER_RIGHT_20_SEQUENCE=TCCTTTCCGCCTGGTTAGAG
PRIMER_LEFT_20=608,21
PRIMER_RIGHT_20=1563,20
PRIMER_LEFT_20_TM=59.915
PRIMER_RIGHT_20_TM=61.620
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=5.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=956
PRIMER_PAIR_21_PENALTY=2.726949
PRIMER_LEFT_21_PENALTY=1.631104
PRIMER_RIGHT_21_PENALTY=1.095845
PRIMER_LEFT_21_SEQUENCE=GGGTTCATATCAGCATCCAA
PRIMER_RIGHT_21_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_21=612,20
PRIMER_RIGHT_21=1565,20
PRIMER_LEFT_21_TM=58.369
PRIMER_RIGHT_21_TM=61.096
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=5.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=954
PRIMER_PAIR_22_PENALTY=2.733260
PRIMER_LEFT_22_PENALTY=1.085102
PRIMER_RIGHT_22_PENALTY=1.648157
PRIMER_LEFT_22_SEQUENCE=TTTCGGGTTCATATCAGCATC
PRIMER_RIGHT_22_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_22=608,21
PRIMER_RIGHT_22=1486,21
PRIMER_LEFT_22_TM=59.915
PRIMER_RIGHT_22_TM=59.352
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.1000
PRIMER_RIGHT_22_END_STABILITY=10.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=879
PRIMER_PAIR_23_PENALTY=2.747678
PRIMER_LEFT_23_PENALTY=1.651834
PRIMER_RIGHT_23_PENALTY=1.095845
PRIMER_LEFT_23_SEQUENCE=AAATCCTCGCCATTCCAAAA
PRIMER_RIGHT_23_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_23=580,20
PRIMER_RIGHT_23=1565,20
PRIMER_LEFT_23_TM=61.652
PRIMER_RIGHT_23_TM=61.096
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.6000
PRIMER_RIGHT_23_END_STABILITY=5.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=986
PRIMER_PAIR_24_PENALTY=2.747678
PRIMER_LEFT_24_PENALTY=1.651834
PRIMER_RIGHT_24_PENALTY=1.095845
PRIMER_LEFT_24_SEQUENCE=AAAATCCTCGCCATTCCAAA
PRIMER_RIGHT_24_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_24=579,20
PRIMER_RIGHT_24=1565,20
PRIMER_LEFT_24_TM=61.652
PRIMER_RIGHT_24_TM=61.096
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.8000
PRIMER_RIGHT_24_END_STABILITY=5.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=987
PRIMER_PAIR_25_PENALTY=2.777936
PRIMER_LEFT_25_PENALTY=1.157635
PRIMER_RIGHT_25_PENALTY=1.620302
PRIMER_LEFT_25_SEQUENCE=ATCCTCGCCATTCCAAAATC
PRIMER_RIGHT_25_SEQUENCE=TCCTTTCCGCCTGGTTAGAG
PRIMER_LEFT_25=582,20
PRIMER_RIGHT_25=1563,20
PRIMER_LEFT_25_TM=61.158
PRIMER_RIGHT_25_TM=61.620
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.9000
PRIMER_RIGHT_25_END_STABILITY=5.7000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=982
PRIMER_PAIR_26_PENALTY=2.781199
PRIMER_LEFT_26_PENALTY=0.825798
PRIMER_RIGHT_26_PENALTY=1.955401
PRIMER_LEFT_26_SEQUENCE=AATCTGCAACTTTCGGGTTC
PRIMER_RIGHT_26_SEQUENCE=CTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_26=598,20
PRIMER_RIGHT_26=1484,20
PRIMER_LEFT_26_TM=59.174
PRIMER_RIGHT_26_TM=58.045
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=8.8000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=887
PRIMER_PAIR_27_PENALTY=2.797804
PRIMER_LEFT_27_PENALTY=0.825798
PRIMER_RIGHT_27_PENALTY=1.972007
PRIMER_LEFT_27_SEQUENCE=AATCTGCAACTTTCGGGTTC
PRIMER_RIGHT_27_SEQUENCE=TTCCTTTCCGCCTGGTTAGA
PRIMER_LEFT_27=598,20
PRIMER_RIGHT_27=1564,20
PRIMER_LEFT_27_TM=59.174
PRIMER_RIGHT_27_TM=61.972
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=6.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=967
PRIMER_PAIR_28_PENALTY=2.805792
PRIMER_LEFT_28_PENALTY=1.157635
PRIMER_RIGHT_28_PENALTY=1.648157
PRIMER_LEFT_28_SEQUENCE=ATCCTCGCCATTCCAAAATC
PRIMER_RIGHT_28_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_28=582,20
PRIMER_RIGHT_28=1486,21
PRIMER_LEFT_28_TM=61.158
PRIMER_RIGHT_28_TM=59.352
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=905
PRIMER_PAIR_29_PENALTY=2.844081
PRIMER_LEFT_29_PENALTY=1.223779
PRIMER_RIGHT_29_PENALTY=1.620302
PRIMER_LEFT_29_SEQUENCE=GGGTTCATATCAGCATCCAAA
PRIMER_RIGHT_29_SEQUENCE=TCCTTTCCGCCTGGTTAGAG
PRIMER_LEFT_29=612,21
PRIMER_RIGHT_29=1563,20
PRIMER_LEFT_29_TM=59.776
PRIMER_RIGHT_29_TM=61.620
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.8000
PRIMER_RIGHT_29_END_STABILITY=5.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=952
PRIMER_PAIR_30_PENALTY=2.854595
PRIMER_LEFT_30_PENALTY=0.825798
PRIMER_RIGHT_30_PENALTY=2.028797
PRIMER_LEFT_30_SEQUENCE=AATCTGCAACTTTCGGGTTC
PRIMER_RIGHT_30_SEQUENCE=GACTTATGCAGGAAAAGGCA
PRIMER_LEFT_30=598,20
PRIMER_RIGHT_30=1486,20
PRIMER_LEFT_30_TM=59.174
PRIMER_RIGHT_30_TM=57.971
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=889
PRIMER_PAIR_31_PENALTY=2.871937
PRIMER_LEFT_31_PENALTY=1.223779
PRIMER_RIGHT_31_PENALTY=1.648157
PRIMER_LEFT_31_SEQUENCE=GGGTTCATATCAGCATCCAAA
PRIMER_RIGHT_31_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_31=612,21
PRIMER_RIGHT_31=1486,21
PRIMER_LEFT_31_TM=59.776
PRIMER_RIGHT_31_TM=59.352
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.8000
PRIMER_RIGHT_31_END_STABILITY=10.0000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=875
PRIMER_PAIR_32_PENALTY=2.892535
PRIMER_LEFT_32_PENALTY=0.636033
PRIMER_RIGHT_32_PENALTY=2.256503
PRIMER_LEFT_32_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_32_SEQUENCE=AGACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_32=599,20
PRIMER_RIGHT_32=1487,22
PRIMER_LEFT_32_TM=60.636
PRIMER_RIGHT_32_TM=60.257
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=10.0000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=889
PRIMER_PAIR_33_PENALTY=2.903321
PRIMER_LEFT_33_PENALTY=1.807476
PRIMER_RIGHT_33_PENALTY=1.095845
PRIMER_LEFT_33_SEQUENCE=CGGGTTCATATCAGCATCCA
PRIMER_RIGHT_33_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_33=611,20
PRIMER_RIGHT_33=1565,20
PRIMER_LEFT_33_TM=61.807
PRIMER_RIGHT_33_TM=61.096
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=955
PRIMER_PAIR_34_PENALTY=2.903832
PRIMER_LEFT_34_PENALTY=1.255675
PRIMER_RIGHT_34_PENALTY=1.648157
PRIMER_LEFT_34_SEQUENCE=TCTCGTATGGGCTTCTGTTTG
PRIMER_RIGHT_34_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_34=548,21
PRIMER_RIGHT_34=1486,21
PRIMER_LEFT_34_TM=60.256
PRIMER_RIGHT_34_TM=59.352
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=10.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=939
PRIMER_PAIR_35_PENALTY=2.976106
PRIMER_LEFT_35_PENALTY=1.020705
PRIMER_RIGHT_35_PENALTY=1.955401
PRIMER_LEFT_35_SEQUENCE=TCGTATGGGCTTCTGTTTGG
PRIMER_RIGHT_35_SEQUENCE=CTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_35=550,20
PRIMER_RIGHT_35=1484,20
PRIMER_LEFT_35_TM=61.021
PRIMER_RIGHT_35_TM=58.045
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.8000
PRIMER_RIGHT_35_END_STABILITY=8.8000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=935
PRIMER_PAIR_36_PENALTY=3.040504
PRIMER_LEFT_36_PENALTY=1.085102
PRIMER_RIGHT_36_PENALTY=1.955401
PRIMER_LEFT_36_SEQUENCE=TTTCGGGTTCATATCAGCATC
PRIMER_RIGHT_36_SEQUENCE=CTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_36=608,21
PRIMER_RIGHT_36=1484,20
PRIMER_LEFT_36_TM=59.915
PRIMER_RIGHT_36_TM=58.045
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=8.8000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=877
PRIMER_PAIR_37_PENALTY=3.049502
PRIMER_LEFT_37_PENALTY=1.020705
PRIMER_RIGHT_37_PENALTY=2.028797
PRIMER_LEFT_37_SEQUENCE=TCGTATGGGCTTCTGTTTGG
PRIMER_RIGHT_37_SEQUENCE=GACTTATGCAGGAAAAGGCA
PRIMER_LEFT_37=550,20
PRIMER_RIGHT_37=1486,20
PRIMER_LEFT_37_TM=61.021
PRIMER_RIGHT_37_TM=57.971
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.8000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=937
PRIMER_PAIR_38_PENALTY=3.057109
PRIMER_LEFT_38_PENALTY=1.085102
PRIMER_RIGHT_38_PENALTY=1.972007
PRIMER_LEFT_38_SEQUENCE=TTTCGGGTTCATATCAGCATC
PRIMER_RIGHT_38_SEQUENCE=TTCCTTTCCGCCTGGTTAGA
PRIMER_LEFT_38=608,21
PRIMER_RIGHT_38=1564,20
PRIMER_LEFT_38_TM=59.915
PRIMER_RIGHT_38_TM=61.972
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=6.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=957
PRIMER_PAIR_39_PENALTY=3.082300
PRIMER_LEFT_39_PENALTY=0.825798
PRIMER_RIGHT_39_PENALTY=2.256503
PRIMER_LEFT_39_SEQUENCE=AATCTGCAACTTTCGGGTTC
PRIMER_RIGHT_39_SEQUENCE=AGACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_39=598,20
PRIMER_RIGHT_39=1487,22
PRIMER_LEFT_39_TM=59.174
PRIMER_RIGHT_39_TM=60.257
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=10.0000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=890
PRIMER_PAIR_40_PENALTY=3.098841
PRIMER_LEFT_40_PENALTY=1.478540
PRIMER_RIGHT_40_PENALTY=1.620302
PRIMER_LEFT_40_SEQUENCE=TTCGGGTTCATATCAGCATC
PRIMER_RIGHT_40_SEQUENCE=TCCTTTCCGCCTGGTTAGAG
PRIMER_LEFT_40=609,20
PRIMER_RIGHT_40=1563,20
PRIMER_LEFT_40_TM=58.521
PRIMER_RIGHT_40_TM=61.620
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=5.7000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=955
PRIMER_PAIR_41_PENALTY=3.113036
PRIMER_LEFT_41_PENALTY=1.157635
PRIMER_RIGHT_41_PENALTY=1.955401
PRIMER_LEFT_41_SEQUENCE=ATCCTCGCCATTCCAAAATC
PRIMER_RIGHT_41_SEQUENCE=CTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_41=582,20
PRIMER_RIGHT_41=1484,20
PRIMER_LEFT_41_TM=61.158
PRIMER_RIGHT_41_TM=58.045
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.9000
PRIMER_RIGHT_41_END_STABILITY=8.8000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=903
PRIMER_PAIR_42_PENALTY=3.113899
PRIMER_LEFT_42_PENALTY=1.085102
PRIMER_RIGHT_42_PENALTY=2.028797
PRIMER_LEFT_42_SEQUENCE=TTTCGGGTTCATATCAGCATC
PRIMER_RIGHT_42_SEQUENCE=GACTTATGCAGGAAAAGGCA
PRIMER_LEFT_42=608,21
PRIMER_RIGHT_42=1486,20
PRIMER_LEFT_42_TM=59.915
PRIMER_RIGHT_42_TM=57.971
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.1000
PRIMER_RIGHT_42_END_STABILITY=9.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=879
PRIMER_PAIR_43_PENALTY=3.120938
PRIMER_LEFT_43_PENALTY=0.636033
PRIMER_RIGHT_43_PENALTY=2.484905
PRIMER_LEFT_43_SEQUENCE=ATCTGCAACTTTCGGGTTCA
PRIMER_RIGHT_43_SEQUENCE=ACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_43=599,20
PRIMER_RIGHT_43=1485,20
PRIMER_LEFT_43_TM=60.636
PRIMER_RIGHT_43_TM=57.515
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=10.0000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=887
PRIMER_PAIR_44_PENALTY=3.126697
PRIMER_LEFT_44_PENALTY=1.478540
PRIMER_RIGHT_44_PENALTY=1.648157
PRIMER_LEFT_44_SEQUENCE=TTCGGGTTCATATCAGCATC
PRIMER_RIGHT_44_SEQUENCE=GACTTATGCAGGAAAAGGCAA
PRIMER_LEFT_44=609,20
PRIMER_RIGHT_44=1486,21
PRIMER_LEFT_44_TM=58.521
PRIMER_RIGHT_44_TM=59.352
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=10.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=878
PRIMER_PAIR_45_PENALTY=3.129641
PRIMER_LEFT_45_PENALTY=1.157635
PRIMER_RIGHT_45_PENALTY=1.972007
PRIMER_LEFT_45_SEQUENCE=ATCCTCGCCATTCCAAAATC
PRIMER_RIGHT_45_SEQUENCE=TTCCTTTCCGCCTGGTTAGA
PRIMER_LEFT_45=582,20
PRIMER_RIGHT_45=1564,20
PRIMER_LEFT_45_TM=61.158
PRIMER_RIGHT_45_TM=61.972
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=6.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=983
PRIMER_PAIR_46_PENALTY=3.173227
PRIMER_LEFT_46_PENALTY=2.077382
PRIMER_RIGHT_46_PENALTY=1.095845
PRIMER_LEFT_46_SEQUENCE=AATCCAAGAAGCTTGACCAGA
PRIMER_RIGHT_46_SEQUENCE=CTTCCTTTCCGCCTGGTTAG
PRIMER_LEFT_46=917,21
PRIMER_RIGHT_46=1565,20
PRIMER_LEFT_46_TM=58.923
PRIMER_RIGHT_46_TM=61.096
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=5.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=649
PRIMER_PAIR_47_PENALTY=3.179181
PRIMER_LEFT_47_PENALTY=1.223779
PRIMER_RIGHT_47_PENALTY=1.955401
PRIMER_LEFT_47_SEQUENCE=GGGTTCATATCAGCATCCAAA
PRIMER_RIGHT_47_SEQUENCE=CTTATGCAGGAAAAGGCAAA
PRIMER_LEFT_47=612,21
PRIMER_RIGHT_47=1484,20
PRIMER_LEFT_47_TM=59.776
PRIMER_RIGHT_47_TM=58.045
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.8000
PRIMER_RIGHT_47_END_STABILITY=8.8000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=873
PRIMER_PAIR_48_PENALTY=3.186432
PRIMER_LEFT_48_PENALTY=1.157635
PRIMER_RIGHT_48_PENALTY=2.028797
PRIMER_LEFT_48_SEQUENCE=ATCCTCGCCATTCCAAAATC
PRIMER_RIGHT_48_SEQUENCE=GACTTATGCAGGAAAAGGCA
PRIMER_LEFT_48=582,20
PRIMER_RIGHT_48=1486,20
PRIMER_LEFT_48_TM=61.158
PRIMER_RIGHT_48_TM=57.971
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=6.9000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=905
PRIMER_PAIR_49_PENALTY=3.195786
PRIMER_LEFT_49_PENALTY=1.223779
PRIMER_RIGHT_49_PENALTY=1.972007
PRIMER_LEFT_49_SEQUENCE=GGGTTCATATCAGCATCCAAA
PRIMER_RIGHT_49_SEQUENCE=TTCCTTTCCGCCTGGTTAGA
PRIMER_LEFT_49=612,21
PRIMER_RIGHT_49=1564,20
PRIMER_LEFT_49_TM=59.776
PRIMER_RIGHT_49_TM=61.972
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.8000
PRIMER_RIGHT_49_END_STABILITY=6.0000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=953
=
SEQUENCE_ID=MAGIC.446_3_1864135_1864680_wu-0_ct-1_135529_507157_TRUE
SEQUENCE_TARGET=950,645
SEQUENCE_TEMPLATE=ATCTTTCACCGGTGGTCTCGATTTCCAGTTCTCGCCGGATATATCAGTCTCGAAATAGTCCCCACCAATGCAAACGTTTTTTCCAGATTCGTCTATGGCTTGAAATTTGAATTCATGAATCTCTCCGGCGGTTAACTCCACAGAATCAATCCCGTCGACACCGGGAACTGAAATCTCGACGGTCTTCGCTTCTCTACACGGCGCTCCTCCCGGAGCCAACCAACCTCTCATCAAATTCGCCGTGAAATTCTTCTGAGATCCAGCGGTTATCCACTCGAACTGGACCCGGGTCGAGTTTGAAGTGAGATTCGTCTTCTTCGGAACCAGATTTCGTGGAGCCATCATCAAGGTGAGATTTTGGCGGTGAGGTTTCTCTGATACTAGATTGGGTTTCGTTGACATAAACGGCGACGGAGAAGGACTGATTCGAACAGAGTAAGCATTGAATCTCCATGGCTGAACGAAGCTTTGAATCGAACAACCGTCAATACTCCAAACGACTAACATAAGGAAGCAGACGAGTGCTGTAAGAGCACCAAGCCGCCATTGAAGAAGAGGGCTTGGCCGTAAAATCACCAATTGGCTCGGTACCGGCGGGAAAATCATTCCTTTCTCCGGCATCTGAAGATCCGACAACAAGAAACAAAAACCCAGAAAATGAGGATTCAATAATTTGACGACAAACAAGGCAATTACCGAGAAAAGATTAAAAGTCATAATACTAACTTTATAGTAAGATAATATTAAACTTACTTTAAATGCTTCAATGGCGGATAACTTCTCCAAACTCGTAAGTNNNAGAAGAAGAAGATTAGATGATGACAATGAATGATACACAATCCACAATTGGCANTTTTTTTAATTTTTGGACAAGAAGAACCTTAAAATCCTCCCAGATTTCCAGAAGACAAGGGAGGATTAAAAAGTCTTAGATTTTTCTTGACTAGACAAAACGAAATGTATACTAATAAGTGCAAAGATAAGGAAAAGAACTCAAAATNTGACAGCTAAAATTGATCTCCCAAAGAAAAATTTAGGATTCTGATTTCTCAGTGCAGCCAAAAAAGCAATTTAGAGGAGGCGGACGGAGAGAAAAAGACAATTTTNNGGATTGACGGTTATACCCCTGATGTTTACTGGATTTACCATGACACCAACCCGACGACCGCGCTAAATAATTGCCCAGCGGACATGTGGGCAACGAAGTCGCGTCGGTGTGCCGGTAGGGGTACTGTTGTCTTTTNCTTTAAACAATTAATACGTCGTGTATGGTGTTGATTGGCGTCACATGCATTTGTTTTGTTATTGTNCTCTCTCTCTCCACCGTCGCTCCTATTTTCTCTGCAGAAGTGAAAAGAAAAAAAATTGTTTTGTCAAAAAAATGGCTCAATAAANAAGACGATTGTCACTTTAAAAAGAAAATAGTAATTATCCAAATATCAATTTCAGAGATTTTTTNGGCTAAAAAGATAAAAGAGCTAGCTCAGATGTTATAATTATCAGTAAATGGAAAACCGTTGGAGATTTAGTACAACTAACAATTTTNGATAAGACAATATAANANTTTTNTTCTTCTTAGATTAGTAGTTGTCTATTTTCAATACCACTTTAGCTTAATCAAAGTGTCCATTCACATTATTCANAAAAATAGTTCATCTATTTTATTAATCTTCTTACTTTGATTCAAGAATTCNTTTTTTTTTNGTTGTTGCTCAAGATAGAATTTAGCTTATACATAAAAATGATACTTTTTCGCAGTTTTAAAATTCTCTAAGAAACCAAACTGATAAAATTTAAAGCGCAAGAGAATATTAAAGTAAGAAAAAGAGTAAATTATTACAGAATCTCCTCACTAAACGAGCTTATACGTTAATGGCTCTAATCTTGAATATGTTGTTGTTGGCCGTTGAGGCTGTGTTTAATCATGTGACTTTTAACTCCAAAGCTACCAAAAGTCTTCGGCCCAAGAGAAAAGAGCTACATGAGTACATTCTACATTCTTCAGCCAATAATGAGACTCCCTACTATCTTAANTATTGGGCCGAATATGGGCCTACATTAACTATTTANNNNNNNNNATAGTATTGTTAGCATCTAGCTTTTTATNATTTTGTTTCAGTGAGGAGAAAAACGCTTTCTCAATTATGAACTNTGCATCTTTGTCACTGATTCTTCTCACCTGATCTGTTGCAGATATTTGGTCAAAGAGTTTATTAAAAGATAACATAATATAAAGTGAAAAAGCAATGCTGCTTTCTCTCATTGGTTGTGCATAGAGCAAGGACAGAGAAAAACAGGTTCGTACGTAAGAAACACACATTAACTGTGTTCTGCTTCCTTTGTATTCTCTCACTTTTCTCNTTTTTCAGAATTCAATCTCTGGAAATTTTCTTCCTAAAGANTAAAGAGAACCGTTTGGACCAGATCAGCTTGCCTCTTGGTAATGGTCTCTCTTCTCTTTGTTCCCAATTTCTGTAGCTTTCTTCTTTCTCCCTAACTCGTCTGCTCTCTTTGTCTCTTCCTCTCACAGTTCATACGTCTTCCA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.539822
PRIMER_LEFT_0_PENALTY=0.227274
PRIMER_RIGHT_0_PENALTY=4.312548
PRIMER_LEFT_0_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_0_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_0=898,20
PRIMER_RIGHT_0=1861,24
PRIMER_LEFT_0_TM=59.773
PRIMER_RIGHT_0_TM=60.313
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.833
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=964
PRIMER_PAIR_1_PENALTY=4.631072
PRIMER_LEFT_1_PENALTY=0.227274
PRIMER_RIGHT_1_PENALTY=4.403798
PRIMER_LEFT_1_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_1_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_1=898,20
PRIMER_RIGHT_1=1876,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=57.596
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=979
PRIMER_PAIR_2_PENALTY=5.057578
PRIMER_LEFT_2_PENALTY=0.745029
PRIMER_RIGHT_2_PENALTY=4.312548
PRIMER_LEFT_2_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_2_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_2=897,20
PRIMER_RIGHT_2=1861,24
PRIMER_LEFT_2_TM=59.255
PRIMER_RIGHT_2_TM=60.313
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.833
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=965
PRIMER_PAIR_3_PENALTY=5.148828
PRIMER_LEFT_3_PENALTY=0.745029
PRIMER_RIGHT_3_PENALTY=4.403798
PRIMER_LEFT_3_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_3_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_3=897,20
PRIMER_RIGHT_3=1876,22
PRIMER_LEFT_3_TM=59.255
PRIMER_RIGHT_3_TM=57.596
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=980
PRIMER_PAIR_4_PENALTY=5.371865
PRIMER_LEFT_4_PENALTY=1.059316
PRIMER_RIGHT_4_PENALTY=4.312548
PRIMER_LEFT_4_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_4_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_4=899,21
PRIMER_RIGHT_4=1861,24
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=60.313
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=963
PRIMER_PAIR_5_PENALTY=5.398751
PRIMER_LEFT_5_PENALTY=0.227274
PRIMER_RIGHT_5_PENALTY=5.171477
PRIMER_LEFT_5_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_5_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_5=898,20
PRIMER_RIGHT_5=1868,23
PRIMER_LEFT_5_TM=59.773
PRIMER_RIGHT_5_TM=57.829
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=971
PRIMER_PAIR_6_PENALTY=5.447393
PRIMER_LEFT_6_PENALTY=0.227274
PRIMER_RIGHT_6_PENALTY=5.220120
PRIMER_LEFT_6_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_6_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_6=898,20
PRIMER_RIGHT_6=1870,25
PRIMER_LEFT_6_TM=59.773
PRIMER_RIGHT_6_TM=59.780
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=973
PRIMER_PAIR_7_PENALTY=5.463115
PRIMER_LEFT_7_PENALTY=1.059316
PRIMER_RIGHT_7_PENALTY=4.403798
PRIMER_LEFT_7_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_7_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_7=899,21
PRIMER_RIGHT_7=1876,22
PRIMER_LEFT_7_TM=60.059
PRIMER_RIGHT_7_TM=57.596
PRIMER_LEFT_7_GC_PERCENT=47.619
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=978
PRIMER_PAIR_8_PENALTY=5.487331
PRIMER_LEFT_8_PENALTY=0.227274
PRIMER_RIGHT_8_PENALTY=5.260057
PRIMER_LEFT_8_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_8_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_8=898,20
PRIMER_RIGHT_8=1868,24
PRIMER_LEFT_8_TM=59.773
PRIMER_RIGHT_8_TM=58.740
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.833
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=971
PRIMER_PAIR_9_PENALTY=5.543440
PRIMER_LEFT_9_PENALTY=0.227274
PRIMER_RIGHT_9_PENALTY=5.316167
PRIMER_LEFT_9_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_9_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAGA
PRIMER_LEFT_9=898,20
PRIMER_RIGHT_9=1868,25
PRIMER_LEFT_9_TM=59.773
PRIMER_RIGHT_9_TM=60.316
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=44.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=971
PRIMER_PAIR_10_PENALTY=5.610881
PRIMER_LEFT_10_PENALTY=0.227274
PRIMER_RIGHT_10_PENALTY=5.383607
PRIMER_LEFT_10_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_10_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_10=898,20
PRIMER_RIGHT_10=1869,24
PRIMER_LEFT_10_TM=59.773
PRIMER_RIGHT_10_TM=58.616
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=41.667
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=972
PRIMER_PAIR_11_PENALTY=5.656721
PRIMER_LEFT_11_PENALTY=1.344173
PRIMER_RIGHT_11_PENALTY=4.312548
PRIMER_LEFT_11_SEQUENCE=TCCCAGATTTCCAGAAGACAA
PRIMER_RIGHT_11_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_11=890,21
PRIMER_RIGHT_11=1861,24
PRIMER_LEFT_11_TM=59.656
PRIMER_RIGHT_11_TM=60.313
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=45.833
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=972
PRIMER_PAIR_12_PENALTY=5.747971
PRIMER_LEFT_12_PENALTY=1.344173
PRIMER_RIGHT_12_PENALTY=4.403798
PRIMER_LEFT_12_SEQUENCE=TCCCAGATTTCCAGAAGACAA
PRIMER_RIGHT_12_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_12=890,21
PRIMER_RIGHT_12=1876,22
PRIMER_LEFT_12_TM=59.656
PRIMER_RIGHT_12_TM=57.596
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=40.909
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=987
PRIMER_PAIR_13_PENALTY=5.750340
PRIMER_LEFT_13_PENALTY=1.437791
PRIMER_RIGHT_13_PENALTY=4.312548
PRIMER_LEFT_13_SEQUENCE=ATTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_13_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_13=896,21
PRIMER_RIGHT_13=1861,24
PRIMER_LEFT_13_TM=59.562
PRIMER_RIGHT_13_TM=60.313
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.833
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=966
PRIMER_PAIR_14_PENALTY=5.760239
PRIMER_LEFT_14_PENALTY=0.227274
PRIMER_RIGHT_14_PENALTY=5.532965
PRIMER_LEFT_14_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_14_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_14=898,20
PRIMER_RIGHT_14=1869,25
PRIMER_LEFT_14_TM=59.773
PRIMER_RIGHT_14_TM=59.467
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=44.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=972
PRIMER_PAIR_15_PENALTY=5.841589
PRIMER_LEFT_15_PENALTY=1.437791
PRIMER_RIGHT_15_PENALTY=4.403798
PRIMER_LEFT_15_SEQUENCE=ATTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_15_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_15=896,21
PRIMER_RIGHT_15=1876,22
PRIMER_LEFT_15_TM=59.562
PRIMER_RIGHT_15_TM=57.596
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=981
PRIMER_PAIR_16_PENALTY=5.886003
PRIMER_LEFT_16_PENALTY=1.573455
PRIMER_RIGHT_16_PENALTY=4.312548
PRIMER_LEFT_16_SEQUENCE=AAAATCCTCCCAGATTTCCA
PRIMER_RIGHT_16_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_16=883,20
PRIMER_RIGHT_16=1861,24
PRIMER_LEFT_16_TM=58.427
PRIMER_RIGHT_16_TM=60.313
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=45.833
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=979
PRIMER_PAIR_17_PENALTY=5.906226
PRIMER_LEFT_17_PENALTY=1.593677
PRIMER_RIGHT_17_PENALTY=4.312548
PRIMER_LEFT_17_SEQUENCE=AAAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_17_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_17=883,21
PRIMER_RIGHT_17=1861,24
PRIMER_LEFT_17_TM=59.406
PRIMER_RIGHT_17_TM=60.313
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=45.833
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=979
PRIMER_PAIR_18_PENALTY=5.916507
PRIMER_LEFT_18_PENALTY=0.745029
PRIMER_RIGHT_18_PENALTY=5.171477
PRIMER_LEFT_18_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_18_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_18=897,20
PRIMER_RIGHT_18=1868,23
PRIMER_LEFT_18_TM=59.255
PRIMER_RIGHT_18_TM=57.829
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=972
PRIMER_PAIR_19_PENALTY=5.965149
PRIMER_LEFT_19_PENALTY=0.745029
PRIMER_RIGHT_19_PENALTY=5.220120
PRIMER_LEFT_19_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_19_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_19=897,20
PRIMER_RIGHT_19=1870,25
PRIMER_LEFT_19_TM=59.255
PRIMER_RIGHT_19_TM=59.780
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=974
PRIMER_PAIR_20_PENALTY=5.977253
PRIMER_LEFT_20_PENALTY=1.573455
PRIMER_RIGHT_20_PENALTY=4.403798
PRIMER_LEFT_20_SEQUENCE=AAAATCCTCCCAGATTTCCA
PRIMER_RIGHT_20_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_20=883,20
PRIMER_RIGHT_20=1876,22
PRIMER_LEFT_20_TM=58.427
PRIMER_RIGHT_20_TM=57.596
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=994
PRIMER_PAIR_21_PENALTY=5.997475
PRIMER_LEFT_21_PENALTY=1.593677
PRIMER_RIGHT_21_PENALTY=4.403798
PRIMER_LEFT_21_SEQUENCE=AAAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_21_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_21=883,21
PRIMER_RIGHT_21=1876,22
PRIMER_LEFT_21_TM=59.406
PRIMER_RIGHT_21_TM=57.596
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=994
PRIMER_PAIR_22_PENALTY=6.005087
PRIMER_LEFT_22_PENALTY=0.745029
PRIMER_RIGHT_22_PENALTY=5.260057
PRIMER_LEFT_22_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_22_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_22=897,20
PRIMER_RIGHT_22=1868,24
PRIMER_LEFT_22_TM=59.255
PRIMER_RIGHT_22_TM=58.740
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=45.833
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=972
PRIMER_PAIR_23_PENALTY=6.016304
PRIMER_LEFT_23_PENALTY=0.227274
PRIMER_RIGHT_23_PENALTY=5.789030
PRIMER_LEFT_23_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_23_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_23=898,20
PRIMER_RIGHT_23=1870,24
PRIMER_LEFT_23_TM=59.773
PRIMER_RIGHT_23_TM=58.211
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=41.667
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=973
PRIMER_PAIR_24_PENALTY=6.043136
PRIMER_LEFT_24_PENALTY=1.730588
PRIMER_RIGHT_24_PENALTY=4.312548
PRIMER_LEFT_24_SEQUENCE=CTCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_24_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_24=889,21
PRIMER_RIGHT_24=1861,24
PRIMER_LEFT_24_TM=59.269
PRIMER_RIGHT_24_TM=60.313
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=45.833
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=973
PRIMER_PAIR_25_PENALTY=6.061196
PRIMER_LEFT_25_PENALTY=0.745029
PRIMER_RIGHT_25_PENALTY=5.316167
PRIMER_LEFT_25_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_25_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAGA
PRIMER_LEFT_25=897,20
PRIMER_RIGHT_25=1868,25
PRIMER_LEFT_25_TM=59.255
PRIMER_RIGHT_25_TM=60.316
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=44.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=9.4000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=972
PRIMER_PAIR_26_PENALTY=6.111421
PRIMER_LEFT_26_PENALTY=1.798872
PRIMER_RIGHT_26_PENALTY=4.312548
PRIMER_LEFT_26_SEQUENCE=TCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_26_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_26=890,20
PRIMER_RIGHT_26=1861,24
PRIMER_LEFT_26_TM=58.201
PRIMER_RIGHT_26_TM=60.313
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.833
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=6.4000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=972
PRIMER_PAIR_27_PENALTY=6.128637
PRIMER_LEFT_27_PENALTY=0.745029
PRIMER_RIGHT_27_PENALTY=5.383607
PRIMER_LEFT_27_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_27_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_27=897,20
PRIMER_RIGHT_27=1869,24
PRIMER_LEFT_27_TM=59.255
PRIMER_RIGHT_27_TM=58.616
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=41.667
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=973
PRIMER_PAIR_28_PENALTY=6.134386
PRIMER_LEFT_28_PENALTY=1.730588
PRIMER_RIGHT_28_PENALTY=4.403798
PRIMER_LEFT_28_SEQUENCE=CTCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_28_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_28=889,21
PRIMER_RIGHT_28=1876,22
PRIMER_LEFT_28_TM=59.269
PRIMER_RIGHT_28_TM=57.596
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=8.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=988
PRIMER_PAIR_29_PENALTY=6.139153
PRIMER_LEFT_29_PENALTY=1.826605
PRIMER_RIGHT_29_PENALTY=4.312548
PRIMER_LEFT_29_SEQUENCE=CCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_29_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_29=900,20
PRIMER_RIGHT_29=1861,24
PRIMER_LEFT_29_TM=58.173
PRIMER_RIGHT_29_TM=60.313
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=45.833
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=962
PRIMER_PAIR_30_PENALTY=6.158546
PRIMER_LEFT_30_PENALTY=0.227274
PRIMER_RIGHT_30_PENALTY=5.931273
PRIMER_LEFT_30_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_30_SEQUENCE=CGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_30=898,20
PRIMER_RIGHT_30=1868,22
PRIMER_LEFT_30_TM=59.773
PRIMER_RIGHT_30_TM=56.069
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.455
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=971
PRIMER_PAIR_31_PENALTY=6.202670
PRIMER_LEFT_31_PENALTY=1.798872
PRIMER_RIGHT_31_PENALTY=4.403798
PRIMER_LEFT_31_SEQUENCE=TCCCAGATTTCCAGAAGACA
PRIMER_RIGHT_31_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_31=890,20
PRIMER_RIGHT_31=1876,22
PRIMER_LEFT_31_TM=58.201
PRIMER_RIGHT_31_TM=57.596
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=40.909
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=987
PRIMER_PAIR_32_PENALTY=6.230403
PRIMER_LEFT_32_PENALTY=1.826605
PRIMER_RIGHT_32_PENALTY=4.403798
PRIMER_LEFT_32_SEQUENCE=CCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_32_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_32=900,20
PRIMER_RIGHT_32=1876,22
PRIMER_LEFT_32_TM=58.173
PRIMER_RIGHT_32_TM=57.596
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=8.4000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=977
PRIMER_PAIR_33_PENALTY=6.230794
PRIMER_LEFT_33_PENALTY=1.059316
PRIMER_RIGHT_33_PENALTY=5.171477
PRIMER_LEFT_33_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_33_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_33=899,21
PRIMER_RIGHT_33=1868,23
PRIMER_LEFT_33_TM=60.059
PRIMER_RIGHT_33_TM=57.829
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=43.478
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=970
PRIMER_PAIR_34_PENALTY=6.272577
PRIMER_LEFT_34_PENALTY=1.960028
PRIMER_RIGHT_34_PENALTY=4.312548
PRIMER_LEFT_34_SEQUENCE=AAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_34_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_34=884,20
PRIMER_RIGHT_34=1861,24
PRIMER_LEFT_34_TM=58.040
PRIMER_RIGHT_34_TM=60.313
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.833
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=978
PRIMER_PAIR_35_PENALTY=6.277995
PRIMER_LEFT_35_PENALTY=0.745029
PRIMER_RIGHT_35_PENALTY=5.532965
PRIMER_LEFT_35_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_35_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_35=897,20
PRIMER_RIGHT_35=1869,25
PRIMER_LEFT_35_TM=59.255
PRIMER_RIGHT_35_TM=59.467
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=44.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=973
PRIMER_PAIR_36_PENALTY=6.279436
PRIMER_LEFT_36_PENALTY=1.059316
PRIMER_RIGHT_36_PENALTY=5.220120
PRIMER_LEFT_36_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_36_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_36=899,21
PRIMER_RIGHT_36=1870,25
PRIMER_LEFT_36_TM=60.059
PRIMER_RIGHT_36_TM=59.780
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=972
PRIMER_PAIR_37_PENALTY=6.287644
PRIMER_LEFT_37_PENALTY=0.227274
PRIMER_RIGHT_37_PENALTY=6.060370
PRIMER_LEFT_37_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_37_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_37=898,20
PRIMER_RIGHT_37=1869,23
PRIMER_LEFT_37_TM=59.773
PRIMER_RIGHT_37_TM=56.940
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=43.478
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=972
PRIMER_PAIR_38_PENALTY=6.319374
PRIMER_LEFT_38_PENALTY=1.059316
PRIMER_RIGHT_38_PENALTY=5.260057
PRIMER_LEFT_38_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_38_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAG
PRIMER_LEFT_38=899,21
PRIMER_RIGHT_38=1868,24
PRIMER_LEFT_38_TM=60.059
PRIMER_RIGHT_38_TM=58.740
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=45.833
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=970
PRIMER_PAIR_39_PENALTY=6.351709
PRIMER_LEFT_39_PENALTY=0.227274
PRIMER_RIGHT_39_PENALTY=6.124435
PRIMER_LEFT_39_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_39_SEQUENCE=AGCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_39=898,20
PRIMER_RIGHT_39=1862,25
PRIMER_LEFT_39_TM=59.773
PRIMER_RIGHT_39_TM=61.124
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=44.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=965
PRIMER_PAIR_40_PENALTY=6.363826
PRIMER_LEFT_40_PENALTY=1.960028
PRIMER_RIGHT_40_PENALTY=4.403798
PRIMER_LEFT_40_SEQUENCE=AAATCCTCCCAGATTTCCAG
PRIMER_RIGHT_40_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_40=884,20
PRIMER_RIGHT_40=1876,22
PRIMER_LEFT_40_TM=58.040
PRIMER_RIGHT_40_TM=57.596
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=8.4000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=993
PRIMER_PAIR_41_PENALTY=6.375483
PRIMER_LEFT_41_PENALTY=1.059316
PRIMER_RIGHT_41_PENALTY=5.316167
PRIMER_LEFT_41_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_41_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGAGA
PRIMER_LEFT_41=899,21
PRIMER_RIGHT_41=1868,25
PRIMER_LEFT_41_TM=60.059
PRIMER_RIGHT_41_TM=60.316
PRIMER_LEFT_41_GC_PERCENT=47.619
PRIMER_RIGHT_41_GC_PERCENT=44.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=970
PRIMER_PAIR_42_PENALTY=6.410738
PRIMER_LEFT_42_PENALTY=0.227274
PRIMER_RIGHT_42_PENALTY=6.183465
PRIMER_LEFT_42_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_42_SEQUENCE=CTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_42=898,20
PRIMER_RIGHT_42=1860,23
PRIMER_LEFT_42_TM=59.773
PRIMER_RIGHT_42_TM=56.817
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=43.478
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=963
PRIMER_PAIR_43_PENALTY=6.420066
PRIMER_LEFT_43_PENALTY=2.107518
PRIMER_RIGHT_43_PENALTY=4.312548
PRIMER_LEFT_43_SEQUENCE=TTTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_43_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_43=897,21
PRIMER_RIGHT_43=1861,24
PRIMER_LEFT_43_TM=61.108
PRIMER_RIGHT_43_TM=60.313
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.833
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=965
PRIMER_PAIR_44_PENALTY=6.442924
PRIMER_LEFT_44_PENALTY=1.059316
PRIMER_RIGHT_44_PENALTY=5.383607
PRIMER_LEFT_44_SEQUENCE=TCCAGAAGACAAGGGAGGATT
PRIMER_RIGHT_44_SEQUENCE=ACGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_44=899,21
PRIMER_RIGHT_44=1869,24
PRIMER_LEFT_44_TM=60.059
PRIMER_RIGHT_44_TM=58.616
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=7.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=971
PRIMER_PAIR_45_PENALTY=6.511316
PRIMER_LEFT_45_PENALTY=2.107518
PRIMER_RIGHT_45_PENALTY=4.403798
PRIMER_LEFT_45_SEQUENCE=TTTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_45_SEQUENCE=GCCATTAACGTATAAGCTCGTT
PRIMER_LEFT_45=897,21
PRIMER_RIGHT_45=1876,22
PRIMER_LEFT_45_TM=61.108
PRIMER_RIGHT_45_TM=57.596
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=980
PRIMER_PAIR_46_PENALTY=6.512234
PRIMER_LEFT_46_PENALTY=0.227274
PRIMER_RIGHT_46_PENALTY=6.284961
PRIMER_LEFT_46_SEQUENCE=TTCCAGAAGACAAGGGAGGA
PRIMER_RIGHT_46_SEQUENCE=TCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_46=898,20
PRIMER_RIGHT_46=1859,22
PRIMER_LEFT_46_TM=59.773
PRIMER_RIGHT_46_TM=55.715
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=962
PRIMER_PAIR_47_PENALTY=6.515650
PRIMER_LEFT_47_PENALTY=1.344173
PRIMER_RIGHT_47_PENALTY=5.171477
PRIMER_LEFT_47_SEQUENCE=TCCCAGATTTCCAGAAGACAA
PRIMER_RIGHT_47_SEQUENCE=CGTATAAGCTCGTTTAGTGAGGA
PRIMER_LEFT_47=890,21
PRIMER_RIGHT_47=1868,23
PRIMER_LEFT_47_TM=59.656
PRIMER_RIGHT_47_TM=57.829
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=43.478
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=7.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=979
PRIMER_PAIR_48_PENALTY=6.534060
PRIMER_LEFT_48_PENALTY=0.745029
PRIMER_RIGHT_48_PENALTY=5.789030
PRIMER_LEFT_48_SEQUENCE=TTTCCAGAAGACAAGGGAGG
PRIMER_RIGHT_48_SEQUENCE=AACGTATAAGCTCGTTTAGTGAGG
PRIMER_LEFT_48=897,20
PRIMER_RIGHT_48=1870,24
PRIMER_LEFT_48_TM=59.255
PRIMER_RIGHT_48_TM=58.211
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=41.667
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=974
PRIMER_PAIR_49_PENALTY=6.536871
PRIMER_LEFT_49_PENALTY=2.224322
PRIMER_RIGHT_49_PENALTY=4.312548
PRIMER_LEFT_49_SEQUENCE=CAGATTTCCAGAAGACAAGGG
PRIMER_RIGHT_49_SEQUENCE=GCTCGTTTAGTGAGGAGATTCTGT
PRIMER_LEFT_49=893,21
PRIMER_RIGHT_49=1861,24
PRIMER_LEFT_49_TM=58.776
PRIMER_RIGHT_49_TM=60.313
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=45.833
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=9.7000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=969
=
SEQUENCE_ID=MAGIC.287_4_5749761_5750059_no-0_mt-0_435571_466787_TRUE
SEQUENCE_TARGET=950,398
SEQUENCE_TEMPLATE=CAAGTTGTCCTATGAAGATTTTTAANAATCTAANNTTTTTTNTGTGATGGTAGTATCAATTTTGAGAATATGTGNATGAATGGAACTTGAATTAGAATTGNTGAAATTTATATGAAGCAAAAAGAGTATTGATNNGATAATGTCAGTACNNNTTTNTTTTTTTNNNNTCATATATAGTGTCAGTACTTTAGGAACACAAAATGATGCTAATTTAAATAGAAGCTAGAATGAAGAAGCACATAATGAAACNAATGTGTATATTTGAAATGAATATAGTATGTTTTAAGATGAGGAAAACAACATGAGGGTGACTTTGTCGNTCATGAGGAGAATGCNAATNATCAAAATACTTATCTGGTAAAGATGAATTTAATNTTTTGTTCATAGAAATCGTAACATTCGATGATGCAATCTCCCCTTCAACTAAACTGAGTCTTGTTCTNTTCATAGATATATGATCATCGTGAATCATTCTTTCTTTCGAACATAAAAGTCTAGTTTTCTTCATAACTTTTGTAGATGATTCATCTCAATAAATGTATGACAAAANANAATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCTCCACTTTCTGCTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANCAANAAAAANAAGCTAGNAAAAAGATAAGCAAGAAGAAGAAAGCGATTTGAGTATTATTCANTCGATTTAAGTACGTGANTCTACTACTCGTATATTTAATNTGAATAAGTTATAATATAATGAAGAAGATNAANNGNAGAATGTTCAANCAATTCNTGTGATCTAACANNNNCTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTCTCGATCCATGNTTACCTCTACTTCAANAANAAAACCTTGGTNANNCTTCNNNNNNNNNNNNNNNNNNNNNNNNNGACCCATCNANAAGAAGATGATANATCAAAAGGTATACGAGCTGAGTAAGNAANAATACATCTCTNNAATTTTTCTTCCAANAATANTTTTACCATTGCTAANATGTTTTGTTTTGGCAGCAAAATCGNACAGAATCAGATCTTTGTGGNNAATATGCATNANACGACAAAAAGTATTATGAAATACAAACTGNCCCACTCGTGATGATCATTGCATGTATTTTATGATANTATANAGAAAGAGTTATTCTCATTGTTTATTTTGTATTANAGATCAAATGTCAGTTAGACTAGATTGNTTTAAATCAGTTTAGTTGATTTATNTAAGAACTCTTGTACAATATCCATTGNAACTTGAATGGCGATATTTTAATTTCTCTTTCCCTCTCNATCTAAGACATTAGAGTAACTAATATGCTATCTTGTTAGGCTATAATGATTTAAGTTGGTTGGTTTAGTTTNTAGTTTGGGTAG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_12660075_12660199_ws-0_zu-0_390_779744_TRUE
SEQUENCE_TARGET=950,224
SEQUENCE_TEMPLATE=GNCAAGATNTATAGTAGCATCCTCGAAAGAGAAACNNATATATATGGTTTATACCATTCAAACAAATTAAACAATATACCTCATTACACTTTAATANGTANTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTACAAGTCAATTAATAATTAGGANTTATATTCAGAGNCNGNCCTCAATAGAAGCTACACAAGCANGTGTTTGTGGCCTNGAATGTGTTAATATTAAATTTAGANGCCNTTAANTATGNAAACTTCCAATATAACTCTATTNTTAAAGACTCGTTAAAGTGAAAAAGAGGTGGTGGATTCAAATGAAAAGNTCCAATAGCTCTATTGTTNAAGACTCCTTTCTNNGAAAATGAGTTGGTGGGTTCGAATTGGAATTCATACCTCAAAGATATTATGGAGAANTNAATAGATATACANNNNAAAGAGTNATGGACNTATTGTATNTGATATTAATTANNGTTTTATACGAACNAAACTAATTGATGGGTTTCATTCTCAATTTTCTTATATNTCTAATGGATCCATGCGCAAATGGTACAAANGCAAATATTACACATTTANATTTATNTACATGATCTCGGAAATTTTNTAANANGCTAATGTCCAAATTAGNNTCTTNGTCNGCTTNNATNNCGNNACNNNNATTNANANNGNACTNGANNNNAAANCNNNGNNNNTAANNCTNCNTNNNNAANTNNTNNNACNNANNTGNNANANCACNNNANNANNNANNANTNTGTNNNCTTTGTNNTTNTNTNNNTNNNTATTNTNATNTTCNTNNNTTCTANNNNGNTTCTNTNATNAGNNTNTNNNANCNANNNNGANNTNNTANNNNNNNNNNTNNNTTATTACTAANNNNGTNTGAAGAATNNANATTNGNNTATTACATTTACNNNTTNTTNTGNAGAATNCAAATTAGTTCANTACNCNAAATNAAATATGTGGTNTANCCACTACNAAAAAGCGGCNCATGACGTCGGTTNTTNTTGACCNTGACGTCGATACTAAACCGACTTNAACAAAATAGCATCAGTTTGAAAANCNATCGTNATAACTTGTGACGTNAAGTATTTTGACACCANTATAACATTCGNTGTTTATTTNTGTGTCTAATATTTGAATCGGTTTAAGTAANTTTAGCGACTGTTTTTATGTGNCACNAAAATCAAAAACTCATTTCTTGCTTTGAAAGTTTCTTAAGTCNTTTTAGATTATGACGTNATNAACCGACNNNTNNATAGATAAGTGGNTGCACACTAGTACCATCAAGTCACCACGCCAACTAACTAAAAGACTCACCTAAAAANCNTCTTTATCTTTAATGTATTCTCTTTGTTCATTTGGTTTTACAANCAAAAAAANNNAAAAANANAACACTTCATGCGTAAGACATAAGCAAAATGGCCGGTGGAGGATTGGCTCTTGATGTCTCCAGCGCCGGAAACATTGATGCCAAGATCACAGCCGCAGTTGTCATGAGTTGCATTGTGGCTGCCTCATGTGGTCTCATTTTTGGCTATGACATCGGAATCTCAGGTNACAAATTCAAGNACATAAAAGGNTCATTTTANNAAACAAGTATATTAGTTAATTTATCTTTGATTATAATTTAACAAAGGGGGTGTGACGACTATGAAGCCGTTTCTTGAGAAATTCTTTCCATCGGTTCTTAANAAAGCTTCGGAAGCTAAGACGAATGTNTACTGCGTCTACGACAGCCAACTTCTTACGGCNTTCACATCTAGCCTTTATGTTGCCGGCTTAGTAGCTTCCTTAGTNGCTAGCCGTCTNACAGCGGCTTATGGTCGCCGAACCACCATGATCCTTGGCGGTTTCACTTTCCTCTTTGGTGCTCTGATCAATGGCTTAGCTGCTAACATCGCCATGCTCATCTCCGGTCGGATTTTGCTAGGATTTGGTGTCGGTTTCACCAACCAGGTAGCNNNNTATAGTTCCAATTTCACTAGN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.815525
PRIMER_LEFT_0_PENALTY=2.573098
PRIMER_RIGHT_0_PENALTY=0.242426
PRIMER_LEFT_0_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_0_SEQUENCE=TTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_0=444,22
PRIMER_RIGHT_0=1441,20
PRIMER_LEFT_0_TM=60.573
PRIMER_RIGHT_0_TM=59.758
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.3000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=998
PRIMER_PAIR_1_PENALTY=2.874246
PRIMER_LEFT_1_PENALTY=2.573098
PRIMER_RIGHT_1_PENALTY=0.301147
PRIMER_LEFT_1_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_1_SEQUENCE=TAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_1=444,22
PRIMER_RIGHT_1=1440,20
PRIMER_LEFT_1_TM=60.573
PRIMER_RIGHT_1_TM=60.301
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=997
PRIMER_PAIR_2_PENALTY=3.322246
PRIMER_LEFT_2_PENALTY=3.079819
PRIMER_RIGHT_2_PENALTY=0.242426
PRIMER_LEFT_2_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_2_SEQUENCE=TTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_2=501,20
PRIMER_RIGHT_2=1441,20
PRIMER_LEFT_2_TM=56.920
PRIMER_RIGHT_2_TM=59.758
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=941
PRIMER_PAIR_3_PENALTY=3.380966
PRIMER_LEFT_3_PENALTY=3.079819
PRIMER_RIGHT_3_PENALTY=0.301147
PRIMER_LEFT_3_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_3_SEQUENCE=TAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_3=501,20
PRIMER_RIGHT_3=1440,20
PRIMER_LEFT_3_TM=56.920
PRIMER_RIGHT_3_TM=60.301
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=940
PRIMER_PAIR_4_PENALTY=4.171112
PRIMER_LEFT_4_PENALTY=2.573098
PRIMER_RIGHT_4_PENALTY=1.598013
PRIMER_LEFT_4_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_4_SEQUENCE=GTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_4=444,22
PRIMER_RIGHT_4=1442,21
PRIMER_LEFT_4_TM=60.573
PRIMER_RIGHT_4_TM=60.598
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=999
PRIMER_PAIR_5_PENALTY=4.677833
PRIMER_LEFT_5_PENALTY=3.079819
PRIMER_RIGHT_5_PENALTY=1.598013
PRIMER_LEFT_5_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_5_SEQUENCE=GTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_5=501,20
PRIMER_RIGHT_5=1442,21
PRIMER_LEFT_5_TM=56.920
PRIMER_RIGHT_5_TM=60.598
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=52.381
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=942
PRIMER_PAIR_6_PENALTY=4.790470
PRIMER_LEFT_6_PENALTY=2.573098
PRIMER_RIGHT_6_PENALTY=2.217372
PRIMER_LEFT_6_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_6_SEQUENCE=AGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_6=444,22
PRIMER_RIGHT_6=1443,21
PRIMER_LEFT_6_TM=60.573
PRIMER_RIGHT_6_TM=58.783
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=1000
PRIMER_PAIR_7_PENALTY=4.844081
PRIMER_LEFT_7_PENALTY=2.573098
PRIMER_RIGHT_7_PENALTY=2.270982
PRIMER_LEFT_7_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_7_SEQUENCE=GTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_7=444,22
PRIMER_RIGHT_7=1442,20
PRIMER_LEFT_7_TM=60.573
PRIMER_RIGHT_7_TM=57.729
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=999
PRIMER_PAIR_8_PENALTY=5.127705
PRIMER_LEFT_8_PENALTY=2.573098
PRIMER_RIGHT_8_PENALTY=2.554607
PRIMER_LEFT_8_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_8_SEQUENCE=GTTGGCGTGGTGACTTGATG
PRIMER_LEFT_8=444,22
PRIMER_RIGHT_8=1438,20
PRIMER_LEFT_8_TM=60.573
PRIMER_RIGHT_8_TM=62.555
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=995
PRIMER_PAIR_9_PENALTY=5.211191
PRIMER_LEFT_9_PENALTY=2.573098
PRIMER_RIGHT_9_PENALTY=2.638093
PRIMER_LEFT_9_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_9_SEQUENCE=TTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_9=444,22
PRIMER_RIGHT_9=1441,21
PRIMER_LEFT_9_TM=60.573
PRIMER_RIGHT_9_TM=61.638
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=998
PRIMER_PAIR_10_PENALTY=5.253145
PRIMER_LEFT_10_PENALTY=2.573098
PRIMER_RIGHT_10_PENALTY=2.680047
PRIMER_LEFT_10_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_10_SEQUENCE=AGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_10=444,22
PRIMER_RIGHT_10=1443,20
PRIMER_LEFT_10_TM=60.573
PRIMER_RIGHT_10_TM=57.320
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=1000
PRIMER_PAIR_11_PENALTY=5.297191
PRIMER_LEFT_11_PENALTY=3.079819
PRIMER_RIGHT_11_PENALTY=2.217372
PRIMER_LEFT_11_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_11_SEQUENCE=AGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_11=501,20
PRIMER_RIGHT_11=1443,21
PRIMER_LEFT_11_TM=56.920
PRIMER_RIGHT_11_TM=58.783
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=943
PRIMER_PAIR_12_PENALTY=5.350801
PRIMER_LEFT_12_PENALTY=3.079819
PRIMER_RIGHT_12_PENALTY=2.270982
PRIMER_LEFT_12_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_12_SEQUENCE=GTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_12=501,20
PRIMER_RIGHT_12=1442,20
PRIMER_LEFT_12_TM=56.920
PRIMER_RIGHT_12_TM=57.729
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=942
PRIMER_PAIR_13_PENALTY=5.394878
PRIMER_LEFT_13_PENALTY=3.079819
PRIMER_RIGHT_13_PENALTY=2.315058
PRIMER_LEFT_13_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_13_SEQUENCE=TTTTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_13=501,20
PRIMER_RIGHT_13=1447,22
PRIMER_LEFT_13_TM=56.920
PRIMER_RIGHT_13_TM=59.685
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=947
PRIMER_PAIR_14_PENALTY=5.634426
PRIMER_LEFT_14_PENALTY=3.079819
PRIMER_RIGHT_14_PENALTY=2.554607
PRIMER_LEFT_14_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_14_SEQUENCE=GTTGGCGTGGTGACTTGATG
PRIMER_LEFT_14=501,20
PRIMER_RIGHT_14=1438,20
PRIMER_LEFT_14_TM=56.920
PRIMER_RIGHT_14_TM=62.555
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=938
PRIMER_PAIR_15_PENALTY=5.717912
PRIMER_LEFT_15_PENALTY=3.079819
PRIMER_RIGHT_15_PENALTY=2.638093
PRIMER_LEFT_15_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_15_SEQUENCE=TTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_15=501,20
PRIMER_RIGHT_15=1441,21
PRIMER_LEFT_15_TM=56.920
PRIMER_RIGHT_15_TM=61.638
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=941
PRIMER_PAIR_16_PENALTY=5.721551
PRIMER_LEFT_16_PENALTY=3.079819
PRIMER_RIGHT_16_PENALTY=2.641731
PRIMER_LEFT_16_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_16_SEQUENCE=TTTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_16=501,20
PRIMER_RIGHT_16=1446,21
PRIMER_LEFT_16_TM=56.920
PRIMER_RIGHT_16_TM=58.358
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=946
PRIMER_PAIR_17_PENALTY=5.759866
PRIMER_LEFT_17_PENALTY=3.079819
PRIMER_RIGHT_17_PENALTY=2.680047
PRIMER_LEFT_17_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_17_SEQUENCE=AGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_17=501,20
PRIMER_RIGHT_17=1443,20
PRIMER_LEFT_17_TM=56.920
PRIMER_RIGHT_17_TM=57.320
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=943
PRIMER_PAIR_18_PENALTY=6.064597
PRIMER_LEFT_18_PENALTY=2.573098
PRIMER_RIGHT_18_PENALTY=3.491498
PRIMER_LEFT_18_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_18_SEQUENCE=AGTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_18=444,22
PRIMER_RIGHT_18=1443,22
PRIMER_LEFT_18_TM=60.573
PRIMER_RIGHT_18_TM=61.491
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=1000
PRIMER_PAIR_19_PENALTY=6.174881
PRIMER_LEFT_19_PENALTY=3.079819
PRIMER_RIGHT_19_PENALTY=3.095061
PRIMER_LEFT_19_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_19_SEQUENCE=TTTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_19=501,20
PRIMER_RIGHT_19=1447,21
PRIMER_LEFT_19_TM=56.920
PRIMER_RIGHT_19_TM=57.905
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=947
PRIMER_PAIR_20_PENALTY=6.181823
PRIMER_LEFT_20_PENALTY=3.079819
PRIMER_RIGHT_20_PENALTY=3.102004
PRIMER_LEFT_20_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_20_SEQUENCE=TTAGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_20=501,20
PRIMER_RIGHT_20=1445,23
PRIMER_LEFT_20_TM=56.920
PRIMER_RIGHT_20_TM=60.102
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=43.478
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=945
PRIMER_PAIR_21_PENALTY=6.181823
PRIMER_LEFT_21_PENALTY=3.079819
PRIMER_RIGHT_21_PENALTY=3.102004
PRIMER_LEFT_21_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_21_SEQUENCE=TTTAGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_21=501,20
PRIMER_RIGHT_21=1446,23
PRIMER_LEFT_21_TM=56.920
PRIMER_RIGHT_21_TM=60.102
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=43.478
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=946
PRIMER_PAIR_22_PENALTY=6.198605
PRIMER_LEFT_22_PENALTY=3.079819
PRIMER_RIGHT_22_PENALTY=3.118785
PRIMER_LEFT_22_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_22_SEQUENCE=TTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_22=501,20
PRIMER_RIGHT_22=1445,20
PRIMER_LEFT_22_TM=56.920
PRIMER_RIGHT_22_TM=56.881
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=2.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=945
PRIMER_PAIR_23_PENALTY=6.203958
PRIMER_LEFT_23_PENALTY=3.079819
PRIMER_RIGHT_23_PENALTY=3.124138
PRIMER_LEFT_23_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_23_SEQUENCE=CTTTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_23=501,20
PRIMER_RIGHT_23=1448,22
PRIMER_LEFT_23_TM=56.920
PRIMER_RIGHT_23_TM=58.876
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.455
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=948
PRIMER_PAIR_24_PENALTY=6.242274
PRIMER_LEFT_24_PENALTY=3.079819
PRIMER_RIGHT_24_PENALTY=3.162454
PRIMER_LEFT_24_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_24_SEQUENCE=TAGTTAGTTGGCGTGGTGACTT
PRIMER_LEFT_24=501,20
PRIMER_RIGHT_24=1444,22
PRIMER_LEFT_24_TM=56.920
PRIMER_RIGHT_24_TM=58.838
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.455
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=944
PRIMER_PAIR_25_PENALTY=6.242274
PRIMER_LEFT_25_PENALTY=3.079819
PRIMER_RIGHT_25_PENALTY=3.162454
PRIMER_LEFT_25_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_25_SEQUENCE=TTAGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_25=501,20
PRIMER_RIGHT_25=1445,22
PRIMER_LEFT_25_TM=56.920
PRIMER_RIGHT_25_TM=58.838
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.455
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=945
PRIMER_PAIR_26_PENALTY=6.571318
PRIMER_LEFT_26_PENALTY=3.079819
PRIMER_RIGHT_26_PENALTY=3.491498
PRIMER_LEFT_26_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_26_SEQUENCE=AGTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_26=501,20
PRIMER_RIGHT_26=1443,22
PRIMER_LEFT_26_TM=56.920
PRIMER_RIGHT_26_TM=61.491
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=943
PRIMER_PAIR_27_PENALTY=6.633844
PRIMER_LEFT_27_PENALTY=3.079819
PRIMER_RIGHT_27_PENALTY=3.554025
PRIMER_LEFT_27_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_27_SEQUENCE=CTTTTAGTTAGTTGGCGTGGTGA
PRIMER_LEFT_27=501,20
PRIMER_RIGHT_27=1448,23
PRIMER_LEFT_27_TM=56.920
PRIMER_RIGHT_27_TM=60.554
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=948
PRIMER_PAIR_28_PENALTY=6.633844
PRIMER_LEFT_28_PENALTY=3.079819
PRIMER_RIGHT_28_PENALTY=3.554025
PRIMER_LEFT_28_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_28_SEQUENCE=TCTTTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_28=501,20
PRIMER_RIGHT_28=1449,23
PRIMER_LEFT_28_TM=56.920
PRIMER_RIGHT_28_TM=60.554
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=43.478
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=949
PRIMER_PAIR_29_PENALTY=6.641268
PRIMER_LEFT_29_PENALTY=3.079819
PRIMER_RIGHT_29_PENALTY=3.561448
PRIMER_LEFT_29_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_29_SEQUENCE=TTTAGTTAGTTGGCGTGGTG
PRIMER_LEFT_29=501,20
PRIMER_RIGHT_29=1446,20
PRIMER_LEFT_29_TM=56.920
PRIMER_RIGHT_29_TM=56.439
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=946
PRIMER_PAIR_30_PENALTY=6.645443
PRIMER_LEFT_30_PENALTY=3.079819
PRIMER_RIGHT_30_PENALTY=3.565623
PRIMER_LEFT_30_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_30_SEQUENCE=TAGTTAGTTGGCGTGGTGACT
PRIMER_LEFT_30=501,20
PRIMER_RIGHT_30=1444,21
PRIMER_LEFT_30_TM=56.920
PRIMER_RIGHT_30_TM=57.434
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=944
PRIMER_PAIR_31_PENALTY=6.923429
PRIMER_LEFT_31_PENALTY=3.079819
PRIMER_RIGHT_31_PENALTY=3.843610
PRIMER_LEFT_31_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_31_SEQUENCE=TCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_31=501,20
PRIMER_RIGHT_31=1449,21
PRIMER_LEFT_31_TM=56.920
PRIMER_RIGHT_31_TM=57.156
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=9.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=949
PRIMER_PAIR_32_PENALTY=6.954178
PRIMER_LEFT_32_PENALTY=2.573098
PRIMER_RIGHT_32_PENALTY=4.381080
PRIMER_LEFT_32_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_32_SEQUENCE=GTTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_32=444,22
PRIMER_RIGHT_32=1442,22
PRIMER_LEFT_32_TM=60.573
PRIMER_RIGHT_32_TM=62.381
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.3000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=999
PRIMER_PAIR_33_PENALTY=6.986088
PRIMER_LEFT_33_PENALTY=2.573098
PRIMER_RIGHT_33_PENALTY=4.412989
PRIMER_LEFT_33_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_33_SEQUENCE=AGTTGGCGTGGTGACTTGATG
PRIMER_LEFT_33=444,22
PRIMER_RIGHT_33=1439,21
PRIMER_LEFT_33_TM=60.573
PRIMER_RIGHT_33_TM=63.413
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=52.381
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=996
PRIMER_PAIR_34_PENALTY=7.063802
PRIMER_LEFT_34_PENALTY=3.079819
PRIMER_RIGHT_34_PENALTY=3.983983
PRIMER_LEFT_34_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_34_SEQUENCE=TCTTTTAGTTAGTTGGCGTGGT
PRIMER_LEFT_34=501,20
PRIMER_RIGHT_34=1449,22
PRIMER_LEFT_34_TM=56.920
PRIMER_RIGHT_34_TM=58.016
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=7.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=949
PRIMER_PAIR_35_PENALTY=7.063802
PRIMER_LEFT_35_PENALTY=3.079819
PRIMER_RIGHT_35_PENALTY=3.983983
PRIMER_LEFT_35_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_35_SEQUENCE=GTCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_35=501,20
PRIMER_RIGHT_35=1450,22
PRIMER_LEFT_35_TM=56.920
PRIMER_RIGHT_35_TM=58.016
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.9000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=950
PRIMER_PAIR_36_PENALTY=7.139041
PRIMER_LEFT_36_PENALTY=3.079819
PRIMER_RIGHT_36_PENALTY=4.059222
PRIMER_LEFT_36_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_36_SEQUENCE=AGTCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_36=501,20
PRIMER_RIGHT_36=1451,23
PRIMER_LEFT_36_TM=56.920
PRIMER_RIGHT_36_TM=58.941
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=43.478
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.9000
PRIMER_RIGHT_36_END_STABILITY=9.9000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=951
PRIMER_PAIR_37_PENALTY=7.197488
PRIMER_LEFT_37_PENALTY=3.079819
PRIMER_RIGHT_37_PENALTY=4.117668
PRIMER_LEFT_37_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_37_SEQUENCE=TGAGTCTTTTAGTTAGTTGGCGTG
PRIMER_LEFT_37=501,20
PRIMER_RIGHT_37=1453,24
PRIMER_LEFT_37_TM=56.920
PRIMER_RIGHT_37_TM=59.882
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=41.667
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=953
PRIMER_PAIR_38_PENALTY=7.220875
PRIMER_LEFT_38_PENALTY=2.318379
PRIMER_RIGHT_38_PENALTY=4.902496
PRIMER_LEFT_38_SEQUENCE=AAGTGAAAAAGAGGTGGTGGA
PRIMER_RIGHT_38_SEQUENCE=TGGTGACTTGATGGTACTAGTGTG
PRIMER_LEFT_38=432,21
PRIMER_RIGHT_38=1431,24
PRIMER_LEFT_38_TM=58.682
PRIMER_RIGHT_38_TM=59.098
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=45.833
PRIMER_LEFT_38_SELF_ANY=1.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=1000
PRIMER_PAIR_39_PENALTY=7.238822
PRIMER_LEFT_39_PENALTY=6.996395
PRIMER_RIGHT_39_PENALTY=0.242426
PRIMER_LEFT_39_SEQUENCE=GAGGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_39_SEQUENCE=TTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_39=442,24
PRIMER_RIGHT_39=1441,20
PRIMER_LEFT_39_TM=62.996
PRIMER_RIGHT_39_TM=59.758
PRIMER_LEFT_39_GC_PERCENT=41.667
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=1000
PRIMER_PAIR_40_PENALTY=7.264061
PRIMER_LEFT_40_PENALTY=3.079819
PRIMER_RIGHT_40_PENALTY=4.184242
PRIMER_LEFT_40_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_40_SEQUENCE=GTCTTTTAGTTAGTTGGCGTGGT
PRIMER_LEFT_40=501,20
PRIMER_RIGHT_40=1450,23
PRIMER_LEFT_40_TM=56.920
PRIMER_RIGHT_40_TM=58.816
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=43.478
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.6000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=950
PRIMER_PAIR_41_PENALTY=7.297543
PRIMER_LEFT_41_PENALTY=6.996395
PRIMER_RIGHT_41_PENALTY=0.301147
PRIMER_LEFT_41_SEQUENCE=GAGGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_41_SEQUENCE=TAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_41=442,24
PRIMER_RIGHT_41=1440,20
PRIMER_LEFT_41_TM=62.996
PRIMER_RIGHT_41_TM=60.301
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.3000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=999
PRIMER_PAIR_42_PENALTY=7.402364
PRIMER_LEFT_42_PENALTY=3.079819
PRIMER_RIGHT_42_PENALTY=4.322545
PRIMER_LEFT_42_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_42_SEQUENCE=AGTCTTTTAGTTAGTTGGCGTGGT
PRIMER_LEFT_42=501,20
PRIMER_RIGHT_42=1451,24
PRIMER_LEFT_42_TM=56.920
PRIMER_RIGHT_42_TM=59.677
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=41.667
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=951
PRIMER_PAIR_43_PENALTY=7.460899
PRIMER_LEFT_43_PENALTY=3.079819
PRIMER_RIGHT_43_PENALTY=4.381080
PRIMER_LEFT_43_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_43_SEQUENCE=GTTAGTTGGCGTGGTGACTTGA
PRIMER_LEFT_43=501,20
PRIMER_RIGHT_43=1442,22
PRIMER_LEFT_43_TM=56.920
PRIMER_RIGHT_43_TM=62.381
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=942
PRIMER_PAIR_44_PENALTY=7.475595
PRIMER_LEFT_44_PENALTY=2.573098
PRIMER_RIGHT_44_PENALTY=4.902496
PRIMER_LEFT_44_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_44_SEQUENCE=TGGTGACTTGATGGTACTAGTGTG
PRIMER_LEFT_44=444,22
PRIMER_RIGHT_44=1431,24
PRIMER_LEFT_44_TM=60.573
PRIMER_RIGHT_44_TM=59.098
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=45.833
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=988
PRIMER_PAIR_45_PENALTY=7.492238
PRIMER_LEFT_45_PENALTY=2.573098
PRIMER_RIGHT_45_PENALTY=4.919140
PRIMER_LEFT_45_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_45_SEQUENCE=TGGCGTGGTGACTTGATGGT
PRIMER_LEFT_45=444,22
PRIMER_RIGHT_45=1436,20
PRIMER_LEFT_45_TM=60.573
PRIMER_RIGHT_45_TM=64.919
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.3000
PRIMER_RIGHT_45_END_STABILITY=7.8000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=993
PRIMER_PAIR_46_PENALTY=7.492808
PRIMER_LEFT_46_PENALTY=3.079819
PRIMER_RIGHT_46_PENALTY=4.412989
PRIMER_LEFT_46_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_46_SEQUENCE=AGTTGGCGTGGTGACTTGATG
PRIMER_LEFT_46=501,20
PRIMER_RIGHT_46=1439,21
PRIMER_LEFT_46_TM=56.920
PRIMER_RIGHT_46_TM=63.413
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=939
PRIMER_PAIR_47_PENALTY=7.528253
PRIMER_LEFT_47_PENALTY=3.079819
PRIMER_RIGHT_47_PENALTY=4.448434
PRIMER_LEFT_47_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_47_SEQUENCE=TAGTTAGTTGGCGTGGTGACTTG
PRIMER_LEFT_47=501,20
PRIMER_RIGHT_47=1444,23
PRIMER_LEFT_47_TM=56.920
PRIMER_RIGHT_47_TM=61.448
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=47.826
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=944
PRIMER_PAIR_48_PENALTY=7.627139
PRIMER_LEFT_48_PENALTY=3.079819
PRIMER_RIGHT_48_PENALTY=4.547320
PRIMER_LEFT_48_SEQUENCE=GAAAATGAGTTGGTGGGTTC
PRIMER_RIGHT_48_SEQUENCE=GAGTCTTTTAGTTAGTTGGCGTGG
PRIMER_LEFT_48=501,20
PRIMER_RIGHT_48=1452,24
PRIMER_LEFT_48_TM=56.920
PRIMER_RIGHT_48_TM=60.547
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.833
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=952
PRIMER_PAIR_49_PENALTY=7.669886
PRIMER_LEFT_49_PENALTY=2.573098
PRIMER_RIGHT_49_PENALTY=5.096788
PRIMER_LEFT_49_SEQUENCE=GGTGGTGGATTCAAATGAAAAG
PRIMER_RIGHT_49_SEQUENCE=GTGGTGACTTGATGGTACTAGTGTG
PRIMER_LEFT_49=444,22
PRIMER_RIGHT_49=1432,25
PRIMER_LEFT_49_TM=60.573
PRIMER_RIGHT_49_TM=59.903
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=48.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=6.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=989
=
SEQUENCE_ID=MAGIC.446_2_4437986_4437996_po-0_bur-0_1482651_34714_TRUE
SEQUENCE_TARGET=950,110
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13139940_13140220_sf-2_ler-0_12406_9533_TRUE
SEQUENCE_TARGET=950,380
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNACNTGACGGTNNAATTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_16423542_16423654_ler-0_ws-0_80512_115438_TRUE
SEQUENCE_TARGET=950,212
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTTTTGAGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGGCCNATGTAATTTCACTCAAAGGCCCATTTAAACCAACCAAACAAATATTGAACGGCTTCAAGCNTCACACNTGAACNCGTGTCAACATCNCATTGCGATTTCCNTTTTTNGTNNNTCTNNTCCAATTTCCATTTTGTTTTGTTATTTCCATATCCTNCTCTATATATACACATAGTATCAATCAATATCATTATCATCACAGATTATACNACTCNTNGNTTCCGAACAAANTAGGGTTTTGTTTTTCCNTTCGATTTCAGATATTTTGTTGTCGATAATGTTTGTAGTAACAAAACATCTTCTGCAAAGACGGAAGAGGTCGTCCTCGTCGTCGTCTTGCTNCNATGATTTCT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_16359277_16359670_can-0_col-0_453196_79226_TRUE
SEQUENCE_TARGET=950,493
SEQUENCE_TEMPLATE=GAGAGACAAACCATGGATGCTTCAGCTCGTTTCTGTCGCGTGTCTCTCATTAGCTGCTAAAGTCGAAGAAACCCAAGTCCCTCTTCTTCTAGACTTTCAAGTAAATGATGATACTTGCAATAAATTTCCTTAAAGTTTAAGTCTTTTNNNNNNNTGTTCTCATCTCTCTNTTTTTTTGGGGGTTTGCAGGTGGAGGAGACAAAGTATGTGTTTGAAGCAAAAACCATACAGAGAATGGAGCTACTGATTCTGTCTACTCTCGAGTGGAAGATGCATCTCATTACTCCAATTTCGTTNGTAGACCACATTATCAGGAGATTGGGACTTAAGAACAATGCTCACTGGGATTTCCTCAACAAATGCCACCGTCTCCTCCTCTCTGTAATCTCCGGTTCGTAAACGACAAAAAGNAAAAAGCCACTCCATTTCGTTTTACCATCTGCTGAATCTGAGTAATCATCTTACATTCTTGTCTTATCTTCCTCAATTGTCTTTGTCAGATTCAAGATTTGTCGGGTACCTCCCATCAGTAGTTGCCGCAGCTACCATGATGCGAATTATAGAGNAAGTTGATCCCTTTGACCCTCTTTCATACCAAACTAATCTCCTCGGTGTCCTTAACTTAACCAAGGTTAGCTTCTCTAATCATCTCTCTACTTGTTTTCNNTCAGNCATTTTCTCAAACAACTCTNGTGCATTAACAGGAAAAGGTGAAAACTTGCTACGATCTAATCCTCCAACTACCAGTGGACCGCATCNGTTTACAGATCCAAATCCAATCTTCCAAGAAACGCAAGAGTCACNATTCATCATCATCGTTGAACAGTCCAAGCTGCGTGATTGATGCAAACCCTTTCAATAGCGACGAAAGCTCAAACGATTCGTGGTCAGCGAGTTCGTGCAACCCACCAACGTCGTCGTCGTCCCCGCAGCANCAACCTCCATTNAAGAAGATGAGAGGAGCTGAAGAGAATGAGAAGAAGAAGCCGATTTTGCATCTNCCATNGGCAATCGTAGCCACTCCATAATCGAAAGCTCGATTTCGTTTATATGATATTTACTGNTTTTTTAAACTTTGAGAACAATCTTTGTTGTATTAAGCNTTACCCGTTTGCATATACGAAATGTCGCGAATGCCCTTACGTGCCATGGCTTGATAGAGTTAATGGGTAAAGGGTATTCATGACATTTGACTGCATGGGATGTGACGAAGGANAGAATTAGANNNAATAATAATAATATTGCGTAAATTTTGAGGCTTGCCCAATCTTTGGGCCGTAATCTCCGGCGTTATTCATNTTTTATTAATGTTTTATATTATGAGTAGTAGTATGTTCTAAAATCTGCTTTGCTGAANNNNNNAANAATTACATANATATTCTTTTACTCCTGTNGTTTAGNNAANAAAAANACGCATTTNAANCCGTGNTANTTGATAANTACANTTNTTTCGTCNAGAATGGTGGATAATACNAAATTTAAGAAATGAGATGTCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTATGATCGAAAGTGAAAGATTTAGCGTTTTTAGCTTTGACTTTTGGGAGAAGAGCCAATTGTNNNNNNNTTTTTTGTCAGTCTTTAAATATAATCGCTTTCAAAGATGAATCAATAATTAAAATTTTAAAACGGAAAGACAAAAATGAAAACGTAACACTATATTATTGCTTTAGATTTTTTGTCTAAAGTCTTGTCAAAACCTGCTCTNCACTATATTATTGCGTATCAAATAACTAACTACACATGANCATGTAAGTTGTCGAAAATAACAACATATAATTATAAATGTTTTTAAAAATCAAACAGCGAAACTTAAATCAAATCTACAGAGTGTAGATCTTTCGTTGACCAGTAGATAAATTAACTGCGACAGACAGACACAGGACAAGAAGGAGGAAGTGAAGTAAACGCGCCATATATAGGTGAGTGTTGTTCGACGACGATAAAAATGTGGTGCAGCATTAGTAACAACACCAGAACCAAAGTAACCTGCTTCCACTCTGAGCACCTGGGATTAAGACACGTGGCAAAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTTAACAATTTACTATTATAAAANCCCTTTTGTATCACGGAAAAGCTTATATGATAATGCGATTGAATCACGAGAAATTNNTNTTTTTTTTTNAAACAAAACTTTNGGTTGTGTGAAAATCTTGGCTCCTTCTTTTACACTTTTTCTTTTCCAATTTTATTTTCTCTTTGGACTTTTTATTTATTTATTTTCTTGGCAATANAAAAAGAGAATTTAAANCTATAACTTTTTTCACGTGTGACACGAAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.187266
PRIMER_LEFT_0_PENALTY=0.003322
PRIMER_RIGHT_0_PENALTY=0.183944
PRIMER_LEFT_0_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_0_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_0=738,20
PRIMER_RIGHT_0=1590,20
PRIMER_LEFT_0_TM=59.997
PRIMER_RIGHT_0_TM=60.184
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.1000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=853
PRIMER_PAIR_1_PENALTY=0.332872
PRIMER_LEFT_1_PENALTY=0.003322
PRIMER_RIGHT_1_PENALTY=0.329550
PRIMER_LEFT_1_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_1_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_1=738,20
PRIMER_RIGHT_1=1591,20
PRIMER_LEFT_1_TM=59.997
PRIMER_RIGHT_1_TM=59.670
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=854
PRIMER_PAIR_2_PENALTY=0.472233
PRIMER_LEFT_2_PENALTY=0.288289
PRIMER_RIGHT_2_PENALTY=0.183944
PRIMER_LEFT_2_SEQUENCE=TCAATAGCGACGAAAGCTCA
PRIMER_RIGHT_2_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_2=855,20
PRIMER_RIGHT_2=1590,20
PRIMER_LEFT_2_TM=59.712
PRIMER_RIGHT_2_TM=60.184
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=736
PRIMER_PAIR_3_PENALTY=0.476358
PRIMER_LEFT_3_PENALTY=0.292414
PRIMER_RIGHT_3_PENALTY=0.183944
PRIMER_LEFT_3_SEQUENCE=AACCCTTTCAATAGCGACGA
PRIMER_RIGHT_3_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_3=848,20
PRIMER_RIGHT_3=1590,20
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=60.184
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=743
PRIMER_PAIR_4_PENALTY=0.598035
PRIMER_LEFT_4_PENALTY=0.003322
PRIMER_RIGHT_4_PENALTY=0.594713
PRIMER_LEFT_4_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_4_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_4=738,20
PRIMER_RIGHT_4=1585,20
PRIMER_LEFT_4_TM=59.997
PRIMER_RIGHT_4_TM=59.405
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=848
PRIMER_PAIR_5_PENALTY=0.598035
PRIMER_LEFT_5_PENALTY=0.003322
PRIMER_RIGHT_5_PENALTY=0.594713
PRIMER_LEFT_5_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_5_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_5=738,20
PRIMER_RIGHT_5=1586,20
PRIMER_LEFT_5_TM=59.997
PRIMER_RIGHT_5_TM=59.405
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=849
PRIMER_PAIR_6_PENALTY=0.618085
PRIMER_LEFT_6_PENALTY=0.434141
PRIMER_RIGHT_6_PENALTY=0.183944
PRIMER_LEFT_6_SEQUENCE=TCCAAGAAACGCAAGAGTCA
PRIMER_RIGHT_6_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_6=782,20
PRIMER_RIGHT_6=1590,20
PRIMER_LEFT_6_TM=59.566
PRIMER_RIGHT_6_TM=60.184
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=809
PRIMER_PAIR_7_PENALTY=0.621964
PRIMER_LEFT_7_PENALTY=0.292414
PRIMER_RIGHT_7_PENALTY=0.329550
PRIMER_LEFT_7_SEQUENCE=AACCCTTTCAATAGCGACGA
PRIMER_RIGHT_7_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_7=848,20
PRIMER_RIGHT_7=1591,20
PRIMER_LEFT_7_TM=59.708
PRIMER_RIGHT_7_TM=59.670
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=10.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=744
PRIMER_PAIR_8_PENALTY=0.763690
PRIMER_LEFT_8_PENALTY=0.434141
PRIMER_RIGHT_8_PENALTY=0.329550
PRIMER_LEFT_8_SEQUENCE=TCCAAGAAACGCAAGAGTCA
PRIMER_RIGHT_8_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_8=782,20
PRIMER_RIGHT_8=1591,20
PRIMER_LEFT_8_TM=59.566
PRIMER_RIGHT_8_TM=59.670
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=810
PRIMER_PAIR_9_PENALTY=0.883002
PRIMER_LEFT_9_PENALTY=0.288289
PRIMER_RIGHT_9_PENALTY=0.594713
PRIMER_LEFT_9_SEQUENCE=TCAATAGCGACGAAAGCTCA
PRIMER_RIGHT_9_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_9=855,20
PRIMER_RIGHT_9=1585,20
PRIMER_LEFT_9_TM=59.712
PRIMER_RIGHT_9_TM=59.405
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=731
PRIMER_PAIR_10_PENALTY=0.883002
PRIMER_LEFT_10_PENALTY=0.288289
PRIMER_RIGHT_10_PENALTY=0.594713
PRIMER_LEFT_10_SEQUENCE=TCAATAGCGACGAAAGCTCA
PRIMER_RIGHT_10_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_10=855,20
PRIMER_RIGHT_10=1586,20
PRIMER_LEFT_10_TM=59.712
PRIMER_RIGHT_10_TM=59.405
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=732
PRIMER_PAIR_11_PENALTY=0.887127
PRIMER_LEFT_11_PENALTY=0.292414
PRIMER_RIGHT_11_PENALTY=0.594713
PRIMER_LEFT_11_SEQUENCE=AACCCTTTCAATAGCGACGA
PRIMER_RIGHT_11_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_11=848,20
PRIMER_RIGHT_11=1585,20
PRIMER_LEFT_11_TM=59.708
PRIMER_RIGHT_11_TM=59.405
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=738
PRIMER_PAIR_12_PENALTY=0.887127
PRIMER_LEFT_12_PENALTY=0.292414
PRIMER_RIGHT_12_PENALTY=0.594713
PRIMER_LEFT_12_SEQUENCE=AACCCTTTCAATAGCGACGA
PRIMER_RIGHT_12_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_12=848,20
PRIMER_RIGHT_12=1586,20
PRIMER_LEFT_12_TM=59.708
PRIMER_RIGHT_12_TM=59.405
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=739
PRIMER_PAIR_13_PENALTY=1.028854
PRIMER_LEFT_13_PENALTY=0.434141
PRIMER_RIGHT_13_PENALTY=0.594713
PRIMER_LEFT_13_SEQUENCE=TCCAAGAAACGCAAGAGTCA
PRIMER_RIGHT_13_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_13=782,20
PRIMER_RIGHT_13=1585,20
PRIMER_LEFT_13_TM=59.566
PRIMER_RIGHT_13_TM=59.405
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=804
PRIMER_PAIR_14_PENALTY=1.028854
PRIMER_LEFT_14_PENALTY=0.434141
PRIMER_RIGHT_14_PENALTY=0.594713
PRIMER_LEFT_14_SEQUENCE=TCCAAGAAACGCAAGAGTCA
PRIMER_RIGHT_14_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_14=782,20
PRIMER_RIGHT_14=1586,20
PRIMER_LEFT_14_TM=59.566
PRIMER_RIGHT_14_TM=59.405
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=805
PRIMER_PAIR_15_PENALTY=1.077902
PRIMER_LEFT_15_PENALTY=0.893958
PRIMER_RIGHT_15_PENALTY=0.183944
PRIMER_LEFT_15_SEQUENCE=CGTGATTGATGCAAACCCTT
PRIMER_RIGHT_15_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_15=835,20
PRIMER_RIGHT_15=1590,20
PRIMER_LEFT_15_TM=60.894
PRIMER_RIGHT_15_TM=60.184
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=8.8000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=756
PRIMER_PAIR_16_PENALTY=1.094562
PRIMER_LEFT_16_PENALTY=0.003322
PRIMER_RIGHT_16_PENALTY=1.091240
PRIMER_LEFT_16_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_16_SEQUENCE=TTGGCTCTTCTCCCAAAAGT
PRIMER_LEFT_16=738,20
PRIMER_RIGHT_16=1587,20
PRIMER_LEFT_16_TM=59.997
PRIMER_RIGHT_16_TM=58.909
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=850
PRIMER_PAIR_17_PENALTY=1.223508
PRIMER_LEFT_17_PENALTY=0.893958
PRIMER_RIGHT_17_PENALTY=0.329550
PRIMER_LEFT_17_SEQUENCE=CGTGATTGATGCAAACCCTT
PRIMER_RIGHT_17_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_17=835,20
PRIMER_RIGHT_17=1591,20
PRIMER_LEFT_17_TM=60.894
PRIMER_RIGHT_17_TM=59.670
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=10.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=757
PRIMER_PAIR_18_PENALTY=1.240826
PRIMER_LEFT_18_PENALTY=0.003322
PRIMER_RIGHT_18_PENALTY=1.237504
PRIMER_LEFT_18_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_18_SEQUENCE=AATTGGCTCTTCTCCCAAAA
PRIMER_LEFT_18=738,20
PRIMER_RIGHT_18=1589,20
PRIMER_LEFT_18_TM=59.997
PRIMER_RIGHT_18_TM=58.762
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=852
PRIMER_PAIR_19_PENALTY=1.292079
PRIMER_LEFT_19_PENALTY=1.108135
PRIMER_RIGHT_19_PENALTY=0.183944
PRIMER_LEFT_19_SEQUENCE=CAATCTTCCAAGAAACGCAA
PRIMER_RIGHT_19_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_19=776,20
PRIMER_RIGHT_19=1590,20
PRIMER_LEFT_19_TM=58.892
PRIMER_RIGHT_19_TM=60.184
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=10.5000
PRIMER_RIGHT_19_END_STABILITY=8.8000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=815
PRIMER_PAIR_20_PENALTY=1.315271
PRIMER_LEFT_20_PENALTY=1.131327
PRIMER_RIGHT_20_PENALTY=0.183944
PRIMER_LEFT_20_SEQUENCE=CAATCTTCCAAGAAACGCAAG
PRIMER_RIGHT_20_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_20=776,21
PRIMER_RIGHT_20=1590,20
PRIMER_LEFT_20_TM=59.869
PRIMER_RIGHT_20_TM=60.184
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.8000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=815
PRIMER_PAIR_21_PENALTY=1.321359
PRIMER_LEFT_21_PENALTY=1.137415
PRIMER_RIGHT_21_PENALTY=0.183944
PRIMER_LEFT_21_SEQUENCE=CCAATCTTCCAAGAAACGCA
PRIMER_RIGHT_21_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_21=775,20
PRIMER_RIGHT_21=1590,20
PRIMER_LEFT_21_TM=61.137
PRIMER_RIGHT_21_TM=60.184
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=8.8000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=816
PRIMER_PAIR_22_PENALTY=1.379529
PRIMER_LEFT_22_PENALTY=0.288289
PRIMER_RIGHT_22_PENALTY=1.091240
PRIMER_LEFT_22_SEQUENCE=TCAATAGCGACGAAAGCTCA
PRIMER_RIGHT_22_SEQUENCE=TTGGCTCTTCTCCCAAAAGT
PRIMER_LEFT_22=855,20
PRIMER_RIGHT_22=1587,20
PRIMER_LEFT_22_TM=59.712
PRIMER_RIGHT_22_TM=58.909
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=733
PRIMER_PAIR_23_PENALTY=1.383654
PRIMER_LEFT_23_PENALTY=0.292414
PRIMER_RIGHT_23_PENALTY=1.091240
PRIMER_LEFT_23_SEQUENCE=AACCCTTTCAATAGCGACGA
PRIMER_RIGHT_23_SEQUENCE=TTGGCTCTTCTCCCAAAAGT
PRIMER_LEFT_23=848,20
PRIMER_RIGHT_23=1587,20
PRIMER_LEFT_23_TM=59.708
PRIMER_RIGHT_23_TM=58.909
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.1000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=740
PRIMER_PAIR_24_PENALTY=1.388559
PRIMER_LEFT_24_PENALTY=0.793846
PRIMER_RIGHT_24_PENALTY=0.594713
PRIMER_LEFT_24_SEQUENCE=CAATAGCGACGAAAGCTCAA
PRIMER_RIGHT_24_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_24=856,20
PRIMER_RIGHT_24=1585,20
PRIMER_LEFT_24_TM=59.206
PRIMER_RIGHT_24_TM=59.405
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=730
PRIMER_PAIR_25_PENALTY=1.388559
PRIMER_LEFT_25_PENALTY=0.793846
PRIMER_RIGHT_25_PENALTY=0.594713
PRIMER_LEFT_25_SEQUENCE=CAATAGCGACGAAAGCTCAA
PRIMER_RIGHT_25_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_25=856,20
PRIMER_RIGHT_25=1586,20
PRIMER_LEFT_25_TM=59.206
PRIMER_RIGHT_25_TM=59.405
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=731
PRIMER_PAIR_26_PENALTY=1.437685
PRIMER_LEFT_26_PENALTY=1.108135
PRIMER_RIGHT_26_PENALTY=0.329550
PRIMER_LEFT_26_SEQUENCE=CAATCTTCCAAGAAACGCAA
PRIMER_RIGHT_26_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_26=776,20
PRIMER_RIGHT_26=1591,20
PRIMER_LEFT_26_TM=58.892
PRIMER_RIGHT_26_TM=59.670
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=10.5000
PRIMER_RIGHT_26_END_STABILITY=10.0000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=816
PRIMER_PAIR_27_PENALTY=1.460877
PRIMER_LEFT_27_PENALTY=1.131327
PRIMER_RIGHT_27_PENALTY=0.329550
PRIMER_LEFT_27_SEQUENCE=CAATCTTCCAAGAAACGCAAG
PRIMER_RIGHT_27_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_27=776,21
PRIMER_RIGHT_27=1591,20
PRIMER_LEFT_27_TM=59.869
PRIMER_RIGHT_27_TM=59.670
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=10.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=816
PRIMER_PAIR_28_PENALTY=1.466965
PRIMER_LEFT_28_PENALTY=1.137415
PRIMER_RIGHT_28_PENALTY=0.329550
PRIMER_LEFT_28_SEQUENCE=CCAATCTTCCAAGAAACGCA
PRIMER_RIGHT_28_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_28=775,20
PRIMER_RIGHT_28=1591,20
PRIMER_LEFT_28_TM=61.137
PRIMER_RIGHT_28_TM=59.670
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.9000
PRIMER_RIGHT_28_END_STABILITY=10.0000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=817
PRIMER_PAIR_29_PENALTY=1.488671
PRIMER_LEFT_29_PENALTY=0.893958
PRIMER_RIGHT_29_PENALTY=0.594713
PRIMER_LEFT_29_SEQUENCE=CGTGATTGATGCAAACCCTT
PRIMER_RIGHT_29_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_29=835,20
PRIMER_RIGHT_29=1585,20
PRIMER_LEFT_29_TM=60.894
PRIMER_RIGHT_29_TM=59.405
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.7000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=751
PRIMER_PAIR_30_PENALTY=1.488671
PRIMER_LEFT_30_PENALTY=0.893958
PRIMER_RIGHT_30_PENALTY=0.594713
PRIMER_LEFT_30_SEQUENCE=CGTGATTGATGCAAACCCTT
PRIMER_RIGHT_30_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_30=835,20
PRIMER_RIGHT_30=1586,20
PRIMER_LEFT_30_TM=60.894
PRIMER_RIGHT_30_TM=59.405
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=752
PRIMER_PAIR_31_PENALTY=1.525380
PRIMER_LEFT_31_PENALTY=0.434141
PRIMER_RIGHT_31_PENALTY=1.091240
PRIMER_LEFT_31_SEQUENCE=TCCAAGAAACGCAAGAGTCA
PRIMER_RIGHT_31_SEQUENCE=TTGGCTCTTCTCCCAAAAGT
PRIMER_LEFT_31=782,20
PRIMER_RIGHT_31=1587,20
PRIMER_LEFT_31_TM=59.566
PRIMER_RIGHT_31_TM=58.909
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=806
PRIMER_PAIR_32_PENALTY=1.525793
PRIMER_LEFT_32_PENALTY=0.288289
PRIMER_RIGHT_32_PENALTY=1.237504
PRIMER_LEFT_32_SEQUENCE=TCAATAGCGACGAAAGCTCA
PRIMER_RIGHT_32_SEQUENCE=AATTGGCTCTTCTCCCAAAA
PRIMER_LEFT_32=855,20
PRIMER_RIGHT_32=1589,20
PRIMER_LEFT_32_TM=59.712
PRIMER_RIGHT_32_TM=58.762
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=735
PRIMER_PAIR_33_PENALTY=1.529918
PRIMER_LEFT_33_PENALTY=0.292414
PRIMER_RIGHT_33_PENALTY=1.237504
PRIMER_LEFT_33_SEQUENCE=AACCCTTTCAATAGCGACGA
PRIMER_RIGHT_33_SEQUENCE=AATTGGCTCTTCTCCCAAAA
PRIMER_LEFT_33=848,20
PRIMER_RIGHT_33=1589,20
PRIMER_LEFT_33_TM=59.708
PRIMER_RIGHT_33_TM=58.762
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=7.6000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=742
PRIMER_PAIR_34_PENALTY=1.636950
PRIMER_LEFT_34_PENALTY=1.453007
PRIMER_RIGHT_34_PENALTY=0.183944
PRIMER_LEFT_34_SEQUENCE=TGATTGATGCAAACCCTTTC
PRIMER_RIGHT_34_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_34=837,20
PRIMER_RIGHT_34=1590,20
PRIMER_LEFT_34_TM=58.547
PRIMER_RIGHT_34_TM=60.184
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=8.8000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=754
PRIMER_PAIR_35_PENALTY=1.671644
PRIMER_LEFT_35_PENALTY=0.434141
PRIMER_RIGHT_35_PENALTY=1.237504
PRIMER_LEFT_35_SEQUENCE=TCCAAGAAACGCAAGAGTCA
PRIMER_RIGHT_35_SEQUENCE=AATTGGCTCTTCTCCCAAAA
PRIMER_LEFT_35=782,20
PRIMER_RIGHT_35=1589,20
PRIMER_LEFT_35_TM=59.566
PRIMER_RIGHT_35_TM=58.762
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=40.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=808
PRIMER_PAIR_36_PENALTY=1.697738
PRIMER_LEFT_36_PENALTY=1.513795
PRIMER_RIGHT_36_PENALTY=0.183944
PRIMER_LEFT_36_SEQUENCE=CAATAGCGACGAAAGCTCAAA
PRIMER_RIGHT_36_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_36=856,21
PRIMER_RIGHT_36=1590,20
PRIMER_LEFT_36_TM=60.514
PRIMER_RIGHT_36_TM=60.184
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.3000
PRIMER_RIGHT_36_END_STABILITY=8.8000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=735
PRIMER_PAIR_37_PENALTY=1.702848
PRIMER_LEFT_37_PENALTY=1.108135
PRIMER_RIGHT_37_PENALTY=0.594713
PRIMER_LEFT_37_SEQUENCE=CAATCTTCCAAGAAACGCAA
PRIMER_RIGHT_37_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_37=776,20
PRIMER_RIGHT_37=1585,20
PRIMER_LEFT_37_TM=58.892
PRIMER_RIGHT_37_TM=59.405
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.5000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=810
PRIMER_PAIR_38_PENALTY=1.702848
PRIMER_LEFT_38_PENALTY=1.108135
PRIMER_RIGHT_38_PENALTY=0.594713
PRIMER_LEFT_38_SEQUENCE=CAATCTTCCAAGAAACGCAA
PRIMER_RIGHT_38_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_38=776,20
PRIMER_RIGHT_38=1586,20
PRIMER_LEFT_38_TM=58.892
PRIMER_RIGHT_38_TM=59.405
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=10.5000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=811
PRIMER_PAIR_39_PENALTY=1.726040
PRIMER_LEFT_39_PENALTY=1.131327
PRIMER_RIGHT_39_PENALTY=0.594713
PRIMER_LEFT_39_SEQUENCE=CAATCTTCCAAGAAACGCAAG
PRIMER_RIGHT_39_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_39=776,21
PRIMER_RIGHT_39=1585,20
PRIMER_LEFT_39_TM=59.869
PRIMER_RIGHT_39_TM=59.405
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=6.4000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=810
PRIMER_PAIR_40_PENALTY=1.731654
PRIMER_LEFT_40_PENALTY=0.003322
PRIMER_RIGHT_40_PENALTY=1.728332
PRIMER_LEFT_40_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_40_SEQUENCE=GGCTCTTCTCCCAAAAGTCAA
PRIMER_LEFT_40=738,20
PRIMER_RIGHT_40=1585,21
PRIMER_LEFT_40_TM=59.997
PRIMER_RIGHT_40_TM=60.728
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=848
PRIMER_PAIR_41_PENALTY=1.731654
PRIMER_LEFT_41_PENALTY=0.003322
PRIMER_RIGHT_41_PENALTY=1.728332
PRIMER_LEFT_41_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_41_SEQUENCE=TTGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_41=738,20
PRIMER_RIGHT_41=1587,21
PRIMER_LEFT_41_TM=59.997
PRIMER_RIGHT_41_TM=60.728
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.1000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=850
PRIMER_PAIR_42_PENALTY=1.732128
PRIMER_LEFT_42_PENALTY=1.137415
PRIMER_RIGHT_42_PENALTY=0.594713
PRIMER_LEFT_42_SEQUENCE=CCAATCTTCCAAGAAACGCA
PRIMER_RIGHT_42_SEQUENCE=GGCTCTTCTCCCAAAAGTCA
PRIMER_LEFT_42=775,20
PRIMER_RIGHT_42=1585,20
PRIMER_LEFT_42_TM=61.137
PRIMER_RIGHT_42_TM=59.405
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=811
PRIMER_PAIR_43_PENALTY=1.732128
PRIMER_LEFT_43_PENALTY=1.137415
PRIMER_RIGHT_43_PENALTY=0.594713
PRIMER_LEFT_43_SEQUENCE=CCAATCTTCCAAGAAACGCA
PRIMER_RIGHT_43_SEQUENCE=TGGCTCTTCTCCCAAAAGTC
PRIMER_LEFT_43=775,20
PRIMER_RIGHT_43=1586,20
PRIMER_LEFT_43_TM=61.137
PRIMER_RIGHT_43_TM=59.405
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=812
PRIMER_PAIR_44_PENALTY=1.759409
PRIMER_LEFT_44_PENALTY=1.575466
PRIMER_RIGHT_44_PENALTY=0.183944
PRIMER_LEFT_44_SEQUENCE=GTGATTGATGCAAACCCTTTC
PRIMER_RIGHT_44_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_44=836,21
PRIMER_RIGHT_44=1590,20
PRIMER_LEFT_44_TM=59.425
PRIMER_RIGHT_44_TM=60.184
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=8.8000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=755
PRIMER_PAIR_45_PENALTY=1.776513
PRIMER_LEFT_45_PENALTY=0.003322
PRIMER_RIGHT_45_PENALTY=1.773191
PRIMER_LEFT_45_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_45_SEQUENCE=ATTGGCTCTTCTCCCAAAAGT
PRIMER_LEFT_45=738,20
PRIMER_RIGHT_45=1588,21
PRIMER_LEFT_45_TM=59.997
PRIMER_RIGHT_45_TM=59.227
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=42.857
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=851
PRIMER_PAIR_46_PENALTY=1.782556
PRIMER_LEFT_46_PENALTY=1.453007
PRIMER_RIGHT_46_PENALTY=0.329550
PRIMER_LEFT_46_SEQUENCE=TGATTGATGCAAACCCTTTC
PRIMER_RIGHT_46_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_46=837,20
PRIMER_RIGHT_46=1591,20
PRIMER_LEFT_46_TM=58.547
PRIMER_RIGHT_46_TM=59.670
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=10.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=755
PRIMER_PAIR_47_PENALTY=1.905015
PRIMER_LEFT_47_PENALTY=1.575466
PRIMER_RIGHT_47_PENALTY=0.329550
PRIMER_LEFT_47_SEQUENCE=GTGATTGATGCAAACCCTTTC
PRIMER_RIGHT_47_SEQUENCE=ACAATTGGCTCTTCTCCCAA
PRIMER_LEFT_47=836,21
PRIMER_RIGHT_47=1591,20
PRIMER_LEFT_47_TM=59.425
PRIMER_RIGHT_47_TM=59.670
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=10.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=756
PRIMER_PAIR_48_PENALTY=1.955677
PRIMER_LEFT_48_PENALTY=1.771733
PRIMER_RIGHT_48_PENALTY=0.183944
PRIMER_LEFT_48_SEQUENCE=TCTTCCAAGAAACGCAAGAG
PRIMER_RIGHT_48_SEQUENCE=CAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_48=779,20
PRIMER_RIGHT_48=1590,20
PRIMER_LEFT_48_TM=58.228
PRIMER_RIGHT_48_TM=60.184
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.7000
PRIMER_RIGHT_48_END_STABILITY=8.8000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=812
PRIMER_PAIR_49_PENALTY=1.975620
PRIMER_LEFT_49_PENALTY=0.003322
PRIMER_RIGHT_49_PENALTY=1.972297
PRIMER_LEFT_49_SEQUENCE=AACTACCAGTGGACCGCATC
PRIMER_RIGHT_49_SEQUENCE=ACAATTGGCTCTTCTCCCAAA
PRIMER_LEFT_49=738,20
PRIMER_RIGHT_49=1591,21
PRIMER_LEFT_49_TM=59.997
PRIMER_RIGHT_49_TM=60.972
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=8.8000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=854
=
SEQUENCE_ID=MAGIC.287_1_15852802_15853083_zu-0_rsch-4_783580_9448_TRUE
SEQUENCE_TARGET=950,381
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_4320860_4321243_wu-0_sf-2_86705_6116_TRUE
SEQUENCE_TARGET=950,483
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4574480_4574875_ler-0_edi-0_70086_29514_TRUE
SEQUENCE_TARGET=950,495
SEQUENCE_TEMPLATE=TTTTTGAAACCAAGTGGTCATATTCATNAGAATTAACACCAAGTATTGGTTTCACTTCAACCTNCAAATATATCNNTCGCAAAATTGTGAGGTANTTGCNAACCGAAAACCCTAAATATTGTTATAACAGAGAAGAGATTCGNTAAANAAANANAATCGAACAAACCCAAATATNAAACTTTAGTNTCATAAANTTNTAACCTTCAATTGATTCCTTTGCTGGAGAAACAAAATTTCTTCAANTCTAAGAAAGAAGACACANTGAGAGAGACGANANTTTTCATAGACCANTCCTATTTTCTCACCAAAGNACATTGANNNNNTNTTCTGCNNNGATACACAAGTGTTTCNANTGCAGGTNTCATTACATGATCAATTCTACCTTCANGTACTTGTTCCACTTCAATCCATCCNTGACTCTAATCCTTCTTGCTNATTTTATACCCTTTGATAACAAGATCAAAANNATGTAATAGATCCTTGTCTTTAGGATTCATNNGTGGNNNNNNNNNNTCATATTCATGAGAATTCACACCTNNNAAAAGGTCCCTCGNAAATTTGCTACAGAATTGNCNGGACTTTGCGAGCAGATAGGAGAACGTCGCTAATTCNTTNCAAATTTTGCGAGGAATATATCCCTCGCAAANNNNNNNNNNNNNNNNNNATTTTCTTCTTNTCTAGTAGAACTNGCAGAAACTCTTTCGATCGCTAAATAGTCCTCCANCTTCTAACTTTGTACCTAAGATCTTTACANTTTTCATAGACAAGTCCTATTTTCNCACCAAAGGACATTGATTTCCTTTTNNNNNNNNNNNCACAAGTGTTTCAATTGCAGGTTTCATTACATGATCAATTCTACCTTNATGTACTTGTTTNACTTCAATCCATCCNTGACTCCAATCNTTCNTGCTCATTTTATANCCTTTGTTCACAAGATCAAAAATANGTAACAGATNCTTGTCTTTANAATTCTTTTTTGAAACCAAGTGGTCATATTCATNAGAATTAATACCAACTATTGGTTTCACTTCAACCTGCAAATATATNNCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCNTAGATATTGNTATAACANAGAAGANATTCGACAAAAANAAATAAANTCGAACAAACCCAAATATCAAACTTTAGTGTCATAAATTTGTTACCTTCAATTGATTCCTTTGCTGGAGANACNAAATTTCTTCAAANTGTTCTAAGNAAGAAGACACANTGAGANAGACGANAGTTTCTCNNAAATTTTTNTTTTACTTTTTGTTACNNTCACANTTNCNNNNTTNTTTTTTTTTTTTTTNGTTTTNAGTGAANTTTNNGCTTTAGTGAATTTNTACTAAAACGGTTNTNTTTCGAAAATAAGAGGTCTAGGCNCAATTACTANCANTTAAAGTGTTAACATTANANTAACTTTTTCAACGCTTTAAAATTCTTTTAGAAACCTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_5476801_5476954_tsu-0_wil-2_6380_20267_TRUE
SEQUENCE_TARGET=950,253
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_7621461_7621699_po-0_ct-1_17036_6588_TRUE
SEQUENCE_TARGET=950,338
SEQUENCE_TEMPLATE=TTATGGCNANGTCGATGTTCTTCAGATGATANATATNCATTATAGTACACAACTCCGTTGATGCAATAATACCNANTTGTAGGTTTATGATCAGGGCTGTTTTNTATCACCCTCCATGATTCTTGAGATCCTAATGTAAGAATATGAGGCTTTTGATANTCGTCCCATAGCGTATTTANATTTGCCACCGATAGAAGTTTATATGTATTCTCCTTAGGATCATATCNTAAATAGATTTTTAGGGGCTNAGAGATTTTAGGTTGAAGCAAGGTTATATGTTCTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNAGNANTANANTTNTNNNNNNNNNAANNTTCTNNNNNNNNCATGNNATGNAANNNGTNNNNATNAGANNNNCTTNTNNNGNTNTCNNGNNATNANANNANGNNNAAGACTNNNNNNNNCTGNNNNNNNNNNNNCATANNANNNNNNNNNNNTTGATGATTGTCGAAGNGCAAATGAATTGATGAAATNTTGTTGGGTGGTTATAGACNACCATAGTTTTGATACACAACGNNATCTTACGATAGATTTTGCAGGNAGTCTCATTAGTATCTCCCNNGTCANATCNATNGGAATATATTCATCAGNAGATACTCCATTACAAGTTGATGAANACTNTGTAATTCTTTGTATTTCNNNNNTCTTCTCTTCTCTTTTTTCCATGGCTCCTAATTTTGCTTCTCTTTTATTTTGTATCTTNANTTNTAACAATGNTNANATATTATGTAGACAGTAGATATGTATGTTCANCATATNCATGTGGATATCTGTTTGTATTAAACTAAAGCACATANGGTTAGATTNTTTTCCCTNAAAACATTTAATTANANNAACTNTTGCATAATAATGTTAGANNTTTTTTGTCTTNANCATTTAATTAGAGAAACTATTGCATACTAATCTANAAAAAAGAGTTNAAACAGTGAAGATTTGTCTANATATNTGAGAACTCAATNTTCACATNTATAAAACCAATCTACAGATGTCCAAATTCTCTACCAATTTAAAGAGACCATAAGACCATACATTGGNGGTTCTCNACTACAAATACTGAATGGTGATATTTATTGACAAACGACTAAANTAGTAAAAGTTGTNNATANACTNCTCTATTCTAATTCTCTAACCGGTTGGTNTAACTTGGTTTAGCCAAACNGAACCCNNNNACTTAGACANAATGCAATNTTCANCGTTGCTTTCAGAATCCTTTTGAGAATTGNNNNNNNNNNNNNNNNNNNACTCTGAAACGAGAGACCTTACACTANNNNNNNNTTCGAANCCGCNTCTTTCTTCTTCTCTGCTTCTTCATCTTTCTTCTTNTTGTGCCGANCAACGTACTTATGGTACTCCTCAAGCTCCTTCTCCAGCTTCTCTATGAACTCACTNGTATGTTCAAGCGATTGCTCATACCGATATTTCTCCAAAGCCTGACGTTTTCATTCACTTTTGTCAAGACAAGAAACAACCAGNCAAANANNAAGTANCTNGATTCANCAATATACCTTATCTTTAGAGAGNGTATCAACAGGAGACATCACTTCTGGTCTCACATAGTTTTTCCCACGGTACAACACAATGGTGTTGTTAGGTTTGACATCAATCACAATGCCCTTACTTAACCGAGCAAGCTCTTCNGCATACTCATGAACCTGCCCNGGTTTGTTGCAGGGCTTGCAAATAACTTTAACAGTCTCATGCTTTTTCCAATGAAGGTGCAAATTCAAAACCACACCTCCAAACACTCCTCGTCTNCCGACAAGTACAAAGTTCTTTCTCTTCTCACCTGTACGTTTTAAGTAATGCTGCTCTTCCTCTGTCAAACTNTCTGGATCATANGGCTCCGCTGGTGATTTCGGNACATCGTATTTCCTCAGTTTCTCAATCAACCATTCTTCTTTCCTCTTAGCCTACACAATCAACCACACCAANANATTTTNAANAAACTCCANNNNNNNNNNNATGTTNCATTGACTAATACAATGNTAAGANGTAACCTTTTCAAGCTTGTAACGAATNCTAACTTCAGGGTTTGGAGAATTCATCTTCTTTTTAGCCTTNAGACGATAAAACCTTAACTCATTCACTTTAGCNTTTCTTGACAATTTAGCCCGTTTCCATTTCTTCTCTTTTCTTGTAGGTACAACANTGTTACCACNACTAGAGACATTGTTGAAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.928432
PRIMER_LEFT_0_PENALTY=0.818040
PRIMER_RIGHT_0_PENALTY=0.110392
PRIMER_LEFT_0_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_0_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_0=722,20
PRIMER_RIGHT_0=1502,20
PRIMER_LEFT_0_TM=59.182
PRIMER_RIGHT_0_TM=60.110
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=781
PRIMER_PAIR_1_PENALTY=0.965335
PRIMER_LEFT_1_PENALTY=0.818040
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_1_SEQUENCE=AAACGTCAGGCTTTGGAGAA
PRIMER_LEFT_1=722,20
PRIMER_RIGHT_1=1498,20
PRIMER_LEFT_1_TM=59.182
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=777
PRIMER_PAIR_2_PENALTY=0.965335
PRIMER_LEFT_2_PENALTY=0.818040
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_2_SEQUENCE=AAAACGTCAGGCTTTGGAGA
PRIMER_LEFT_2=722,20
PRIMER_RIGHT_2=1499,20
PRIMER_LEFT_2_TM=59.182
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=778
PRIMER_PAIR_3_PENALTY=0.965335
PRIMER_LEFT_3_PENALTY=0.818040
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_3_SEQUENCE=GAAAACGTCAGGCTTTGGAG
PRIMER_LEFT_3=722,20
PRIMER_RIGHT_3=1500,20
PRIMER_LEFT_3_TM=59.182
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=779
PRIMER_PAIR_4_PENALTY=1.144961
PRIMER_LEFT_4_PENALTY=0.818040
PRIMER_RIGHT_4_PENALTY=0.326921
PRIMER_LEFT_4_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_4_SEQUENCE=CTGGAGAAGGAGCTTGAGGA
PRIMER_LEFT_4=722,20
PRIMER_RIGHT_4=1429,20
PRIMER_LEFT_4_TM=59.182
PRIMER_RIGHT_4_TM=59.673
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=708
PRIMER_PAIR_5_PENALTY=1.509603
PRIMER_LEFT_5_PENALTY=0.818040
PRIMER_RIGHT_5_PENALTY=0.691563
PRIMER_LEFT_5_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_5_SEQUENCE=AAGCTGGAGAAGGAGCTTGA
PRIMER_LEFT_5=722,20
PRIMER_RIGHT_5=1432,20
PRIMER_LEFT_5_TM=59.182
PRIMER_RIGHT_5_TM=59.308
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=711
PRIMER_PAIR_6_PENALTY=1.555657
PRIMER_LEFT_6_PENALTY=1.445265
PRIMER_RIGHT_6_PENALTY=0.110392
PRIMER_LEFT_6_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_6_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_6=722,21
PRIMER_RIGHT_6=1502,20
PRIMER_LEFT_6_TM=60.445
PRIMER_RIGHT_6_TM=60.110
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=781
PRIMER_PAIR_7_PENALTY=1.592561
PRIMER_LEFT_7_PENALTY=1.445265
PRIMER_RIGHT_7_PENALTY=0.147295
PRIMER_LEFT_7_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_7_SEQUENCE=AAACGTCAGGCTTTGGAGAA
PRIMER_LEFT_7=722,21
PRIMER_RIGHT_7=1498,20
PRIMER_LEFT_7_TM=60.445
PRIMER_RIGHT_7_TM=59.853
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=777
PRIMER_PAIR_8_PENALTY=1.592561
PRIMER_LEFT_8_PENALTY=1.445265
PRIMER_RIGHT_8_PENALTY=0.147295
PRIMER_LEFT_8_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_8_SEQUENCE=AAAACGTCAGGCTTTGGAGA
PRIMER_LEFT_8=722,21
PRIMER_RIGHT_8=1499,20
PRIMER_LEFT_8_TM=60.445
PRIMER_RIGHT_8_TM=59.853
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=778
PRIMER_PAIR_9_PENALTY=1.592561
PRIMER_LEFT_9_PENALTY=1.445265
PRIMER_RIGHT_9_PENALTY=0.147295
PRIMER_LEFT_9_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_9_SEQUENCE=GAAAACGTCAGGCTTTGGAG
PRIMER_LEFT_9=722,21
PRIMER_RIGHT_9=1500,20
PRIMER_LEFT_9_TM=60.445
PRIMER_RIGHT_9_TM=59.853
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=779
PRIMER_PAIR_10_PENALTY=2.045082
PRIMER_LEFT_10_PENALTY=1.934690
PRIMER_RIGHT_10_PENALTY=0.110392
PRIMER_LEFT_10_SEQUENCE=TCCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_10_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_10=721,21
PRIMER_RIGHT_10=1502,20
PRIMER_LEFT_10_TM=60.935
PRIMER_RIGHT_10_TM=60.110
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.8000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=782
PRIMER_PAIR_11_PENALTY=2.069511
PRIMER_LEFT_11_PENALTY=0.818040
PRIMER_RIGHT_11_PENALTY=1.251471
PRIMER_LEFT_11_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_11_SEQUENCE=ATTGTGTTGTACCGTGGGAA
PRIMER_LEFT_11=722,20
PRIMER_RIGHT_11=1639,20
PRIMER_LEFT_11_TM=59.182
PRIMER_RIGHT_11_TM=58.749
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=918
PRIMER_PAIR_12_PENALTY=2.081985
PRIMER_LEFT_12_PENALTY=1.934690
PRIMER_RIGHT_12_PENALTY=0.147295
PRIMER_LEFT_12_SEQUENCE=TCCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_12_SEQUENCE=AAACGTCAGGCTTTGGAGAA
PRIMER_LEFT_12=721,21
PRIMER_RIGHT_12=1498,20
PRIMER_LEFT_12_TM=60.935
PRIMER_RIGHT_12_TM=59.853
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=778
PRIMER_PAIR_13_PENALTY=2.081985
PRIMER_LEFT_13_PENALTY=1.934690
PRIMER_RIGHT_13_PENALTY=0.147295
PRIMER_LEFT_13_SEQUENCE=TCCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_13_SEQUENCE=AAAACGTCAGGCTTTGGAGA
PRIMER_LEFT_13=721,21
PRIMER_RIGHT_13=1499,20
PRIMER_LEFT_13_TM=60.935
PRIMER_RIGHT_13_TM=59.853
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=779
PRIMER_PAIR_14_PENALTY=2.081985
PRIMER_LEFT_14_PENALTY=1.934690
PRIMER_RIGHT_14_PENALTY=0.147295
PRIMER_LEFT_14_SEQUENCE=TCCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_14_SEQUENCE=GAAAACGTCAGGCTTTGGAG
PRIMER_LEFT_14=721,21
PRIMER_RIGHT_14=1500,20
PRIMER_LEFT_14_TM=60.935
PRIMER_RIGHT_14_TM=59.853
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=780
PRIMER_PAIR_15_PENALTY=2.261611
PRIMER_LEFT_15_PENALTY=1.934690
PRIMER_RIGHT_15_PENALTY=0.326921
PRIMER_LEFT_15_SEQUENCE=TCCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_15_SEQUENCE=CTGGAGAAGGAGCTTGAGGA
PRIMER_LEFT_15=721,21
PRIMER_RIGHT_15=1429,20
PRIMER_LEFT_15_TM=60.935
PRIMER_RIGHT_15_TM=59.673
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=709
PRIMER_PAIR_16_PENALTY=2.266856
PRIMER_LEFT_16_PENALTY=2.156464
PRIMER_RIGHT_16_PENALTY=0.110392
PRIMER_LEFT_16_SEQUENCE=CATGGCTCCTAATTTTGCTTC
PRIMER_RIGHT_16_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_16=723,21
PRIMER_RIGHT_16=1502,20
PRIMER_LEFT_16_TM=58.844
PRIMER_RIGHT_16_TM=60.110
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.8000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=780
PRIMER_PAIR_17_PENALTY=2.295661
PRIMER_LEFT_17_PENALTY=0.818040
PRIMER_RIGHT_17_PENALTY=1.477620
PRIMER_LEFT_17_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_17_SEQUENCE=CGGTTAAGTAAGGGCATTGTG
PRIMER_LEFT_17=722,20
PRIMER_RIGHT_17=1684,21
PRIMER_LEFT_17_TM=59.182
PRIMER_RIGHT_17_TM=59.522
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=963
PRIMER_PAIR_18_PENALTY=2.303759
PRIMER_LEFT_18_PENALTY=2.156464
PRIMER_RIGHT_18_PENALTY=0.147295
PRIMER_LEFT_18_SEQUENCE=CATGGCTCCTAATTTTGCTTC
PRIMER_RIGHT_18_SEQUENCE=AAACGTCAGGCTTTGGAGAA
PRIMER_LEFT_18=723,21
PRIMER_RIGHT_18=1498,20
PRIMER_LEFT_18_TM=58.844
PRIMER_RIGHT_18_TM=59.853
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=776
PRIMER_PAIR_19_PENALTY=2.303759
PRIMER_LEFT_19_PENALTY=2.156464
PRIMER_RIGHT_19_PENALTY=0.147295
PRIMER_LEFT_19_SEQUENCE=CATGGCTCCTAATTTTGCTTC
PRIMER_RIGHT_19_SEQUENCE=AAAACGTCAGGCTTTGGAGA
PRIMER_LEFT_19=723,21
PRIMER_RIGHT_19=1499,20
PRIMER_LEFT_19_TM=58.844
PRIMER_RIGHT_19_TM=59.853
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=777
PRIMER_PAIR_20_PENALTY=2.303759
PRIMER_LEFT_20_PENALTY=2.156464
PRIMER_RIGHT_20_PENALTY=0.147295
PRIMER_LEFT_20_SEQUENCE=CATGGCTCCTAATTTTGCTTC
PRIMER_RIGHT_20_SEQUENCE=GAAAACGTCAGGCTTTGGAG
PRIMER_LEFT_20=723,21
PRIMER_RIGHT_20=1500,20
PRIMER_LEFT_20_TM=58.844
PRIMER_RIGHT_20_TM=59.853
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=778
PRIMER_PAIR_21_PENALTY=2.334712
PRIMER_LEFT_21_PENALTY=0.818040
PRIMER_RIGHT_21_PENALTY=1.516672
PRIMER_LEFT_21_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_21_SEQUENCE=TGGAGAAGGAGCTTGAGGAGT
PRIMER_LEFT_21=722,20
PRIMER_RIGHT_21=1428,21
PRIMER_LEFT_21_TM=59.182
PRIMER_RIGHT_21_TM=60.517
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=7.6000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=707
PRIMER_PAIR_22_PENALTY=2.343508
PRIMER_LEFT_22_PENALTY=0.818040
PRIMER_RIGHT_22_PENALTY=1.525468
PRIMER_LEFT_22_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_22_SEQUENCE=CCGTGGGAAAAACTATGTGA
PRIMER_LEFT_22=722,20
PRIMER_RIGHT_22=1628,20
PRIMER_LEFT_22_TM=59.182
PRIMER_RIGHT_22_TM=58.475
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=907
PRIMER_PAIR_23_PENALTY=2.352749
PRIMER_LEFT_23_PENALTY=2.242357
PRIMER_RIGHT_23_PENALTY=0.110392
PRIMER_LEFT_23_SEQUENCE=CATGGCTCCTAATTTTGCTTCT
PRIMER_RIGHT_23_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_23=723,22
PRIMER_RIGHT_23=1502,20
PRIMER_LEFT_23_TM=59.758
PRIMER_RIGHT_23_TM=60.110
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=8.8000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=780
PRIMER_PAIR_24_PENALTY=2.389652
PRIMER_LEFT_24_PENALTY=2.242357
PRIMER_RIGHT_24_PENALTY=0.147295
PRIMER_LEFT_24_SEQUENCE=CATGGCTCCTAATTTTGCTTCT
PRIMER_RIGHT_24_SEQUENCE=AAAACGTCAGGCTTTGGAGA
PRIMER_LEFT_24=723,22
PRIMER_RIGHT_24=1499,20
PRIMER_LEFT_24_TM=59.758
PRIMER_RIGHT_24_TM=59.853
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.7000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PAIR_24_PRODUCT_SIZE=777
PRIMER_PAIR_25_PENALTY=2.389652
PRIMER_LEFT_25_PENALTY=2.242357
PRIMER_RIGHT_25_PENALTY=0.147295
PRIMER_LEFT_25_SEQUENCE=CATGGCTCCTAATTTTGCTTCT
PRIMER_RIGHT_25_SEQUENCE=GAAAACGTCAGGCTTTGGAG
PRIMER_LEFT_25=723,22
PRIMER_RIGHT_25=1500,20
PRIMER_LEFT_25_TM=59.758
PRIMER_RIGHT_25_TM=59.853
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=778
PRIMER_PAIR_26_PENALTY=2.389761
PRIMER_LEFT_26_PENALTY=0.818040
PRIMER_RIGHT_26_PENALTY=1.571721
PRIMER_LEFT_26_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_26_SEQUENCE=GCTGGAGAAGGAGCTTGAGG
PRIMER_LEFT_26=722,20
PRIMER_RIGHT_26=1430,20
PRIMER_LEFT_26_TM=59.182
PRIMER_RIGHT_26_TM=61.572
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=60.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=709
PRIMER_PAIR_27_PENALTY=2.472782
PRIMER_LEFT_27_PENALTY=0.818040
PRIMER_RIGHT_27_PENALTY=1.654741
PRIMER_LEFT_27_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_27_SEQUENCE=ACCGTGGGAAAAACTATGTGA
PRIMER_LEFT_27=722,20
PRIMER_RIGHT_27=1629,21
PRIMER_LEFT_27_TM=59.182
PRIMER_RIGHT_27_TM=59.345
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=908
PRIMER_PAIR_28_PENALTY=2.513255
PRIMER_LEFT_28_PENALTY=0.818040
PRIMER_RIGHT_28_PENALTY=1.695215
PRIMER_LEFT_28_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_28_SEQUENCE=CACCATTGTGTTGTACCGTG
PRIMER_LEFT_28=722,20
PRIMER_RIGHT_28=1643,20
PRIMER_LEFT_28_TM=59.182
PRIMER_RIGHT_28_TM=58.305
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=9.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=922
PRIMER_PAIR_29_PENALTY=2.516983
PRIMER_LEFT_29_PENALTY=2.406591
PRIMER_RIGHT_29_PENALTY=0.110392
PRIMER_LEFT_29_SEQUENCE=TGGCTCCTAATTTTGCTTCTC
PRIMER_RIGHT_29_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_29=725,21
PRIMER_RIGHT_29=1502,20
PRIMER_LEFT_29_TM=58.593
PRIMER_RIGHT_29_TM=60.110
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=8.8000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=778
PRIMER_PAIR_30_PENALTY=2.534069
PRIMER_LEFT_30_PENALTY=0.818040
PRIMER_RIGHT_30_PENALTY=1.716029
PRIMER_LEFT_30_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_30_SEQUENCE=TGAAAACGTCAGGCTTTGGA
PRIMER_LEFT_30=722,20
PRIMER_RIGHT_30=1501,20
PRIMER_LEFT_30_TM=59.182
PRIMER_RIGHT_30_TM=61.716
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.5000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=780
PRIMER_PAIR_31_PENALTY=2.540568
PRIMER_LEFT_31_PENALTY=0.818040
PRIMER_RIGHT_31_PENALTY=1.722528
PRIMER_LEFT_31_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_31_SEQUENCE=AGGCTTTGGAGAAATATCGG
PRIMER_LEFT_31=722,20
PRIMER_RIGHT_31=1491,20
PRIMER_LEFT_31_TM=59.182
PRIMER_RIGHT_31_TM=58.277
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=9.8000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=770
PRIMER_PAIR_32_PENALTY=2.587426
PRIMER_LEFT_32_PENALTY=2.477034
PRIMER_RIGHT_32_PENALTY=0.110392
PRIMER_LEFT_32_SEQUENCE=TGGCTCCTAATTTTGCTTCTCT
PRIMER_RIGHT_32_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_32=725,22
PRIMER_RIGHT_32=1502,20
PRIMER_LEFT_32_TM=59.523
PRIMER_RIGHT_32_TM=60.110
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=8.8000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=778
PRIMER_PAIR_33_PENALTY=2.624329
PRIMER_LEFT_33_PENALTY=2.477034
PRIMER_RIGHT_33_PENALTY=0.147295
PRIMER_LEFT_33_SEQUENCE=TGGCTCCTAATTTTGCTTCTCT
PRIMER_RIGHT_33_SEQUENCE=AAAACGTCAGGCTTTGGAGA
PRIMER_LEFT_33=725,22
PRIMER_RIGHT_33=1499,20
PRIMER_LEFT_33_TM=59.523
PRIMER_RIGHT_33_TM=59.853
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=775
PRIMER_PAIR_34_PENALTY=2.626253
PRIMER_LEFT_34_PENALTY=1.934690
PRIMER_RIGHT_34_PENALTY=0.691563
PRIMER_LEFT_34_SEQUENCE=TCCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_34_SEQUENCE=AAGCTGGAGAAGGAGCTTGA
PRIMER_LEFT_34=721,21
PRIMER_RIGHT_34=1432,20
PRIMER_LEFT_34_TM=60.935
PRIMER_RIGHT_34_TM=59.308
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=712
PRIMER_PAIR_35_PENALTY=2.696736
PRIMER_LEFT_35_PENALTY=1.445265
PRIMER_RIGHT_35_PENALTY=1.251471
PRIMER_LEFT_35_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_35_SEQUENCE=ATTGTGTTGTACCGTGGGAA
PRIMER_LEFT_35=722,21
PRIMER_RIGHT_35=1639,20
PRIMER_LEFT_35_TM=60.445
PRIMER_RIGHT_35_TM=58.749
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=918
PRIMER_PAIR_36_PENALTY=2.742831
PRIMER_LEFT_36_PENALTY=0.818040
PRIMER_RIGHT_36_PENALTY=1.924791
PRIMER_LEFT_36_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_36_SEQUENCE=CAGGCTTTGGAGAAATATCGG
PRIMER_LEFT_36=722,20
PRIMER_RIGHT_36=1492,21
PRIMER_LEFT_36_TM=59.182
PRIMER_RIGHT_36_TM=60.925
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.5000
PRIMER_RIGHT_36_END_STABILITY=9.8000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=771
PRIMER_PAIR_37_PENALTY=2.848027
PRIMER_LEFT_37_PENALTY=2.156464
PRIMER_RIGHT_37_PENALTY=0.691563
PRIMER_LEFT_37_SEQUENCE=CATGGCTCCTAATTTTGCTTC
PRIMER_RIGHT_37_SEQUENCE=AAGCTGGAGAAGGAGCTTGA
PRIMER_LEFT_37=723,21
PRIMER_RIGHT_37=1432,20
PRIMER_LEFT_37_TM=58.844
PRIMER_RIGHT_37_TM=59.308
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=710
PRIMER_PAIR_38_PENALTY=2.875705
PRIMER_LEFT_38_PENALTY=0.818040
PRIMER_RIGHT_38_PENALTY=2.057665
PRIMER_LEFT_38_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_38_SEQUENCE=CCATTGTGTTGTACCGTGGG
PRIMER_LEFT_38=722,20
PRIMER_RIGHT_38=1641,20
PRIMER_LEFT_38_TM=59.182
PRIMER_RIGHT_38_TM=62.058
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=920
PRIMER_PAIR_39_PENALTY=2.883985
PRIMER_LEFT_39_PENALTY=0.818040
PRIMER_RIGHT_39_PENALTY=2.065945
PRIMER_LEFT_39_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_39_SEQUENCE=TGAGCAATCGCTTGAACATAC
PRIMER_LEFT_39=722,20
PRIMER_RIGHT_39=1469,21
PRIMER_LEFT_39_TM=59.182
PRIMER_RIGHT_39_TM=58.934
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=5.6000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=748
PRIMER_PAIR_40_PENALTY=2.922886
PRIMER_LEFT_40_PENALTY=1.445265
PRIMER_RIGHT_40_PENALTY=1.477620
PRIMER_LEFT_40_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_40_SEQUENCE=CGGTTAAGTAAGGGCATTGTG
PRIMER_LEFT_40=722,21
PRIMER_RIGHT_40=1684,21
PRIMER_LEFT_40_TM=60.445
PRIMER_RIGHT_40_TM=59.522
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=963
PRIMER_PAIR_41_PENALTY=2.933920
PRIMER_LEFT_41_PENALTY=2.242357
PRIMER_RIGHT_41_PENALTY=0.691563
PRIMER_LEFT_41_SEQUENCE=CATGGCTCCTAATTTTGCTTCT
PRIMER_RIGHT_41_SEQUENCE=AAGCTGGAGAAGGAGCTTGA
PRIMER_LEFT_41=723,22
PRIMER_RIGHT_41=1432,20
PRIMER_LEFT_41_TM=59.758
PRIMER_RIGHT_41_TM=59.308
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=710
PRIMER_PAIR_42_PENALTY=2.947060
PRIMER_LEFT_42_PENALTY=0.818040
PRIMER_RIGHT_42_PENALTY=2.129020
PRIMER_LEFT_42_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_42_SEQUENCE=AAAACGTCAGGCTTTGGAGAA
PRIMER_LEFT_42=722,20
PRIMER_RIGHT_42=1499,21
PRIMER_LEFT_42_TM=59.182
PRIMER_RIGHT_42_TM=61.129
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.5000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=778
PRIMER_PAIR_43_PENALTY=2.955243
PRIMER_LEFT_43_PENALTY=0.818040
PRIMER_RIGHT_43_PENALTY=2.137203
PRIMER_LEFT_43_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_43_SEQUENCE=AAGGGCATTGTGATTGATGT
PRIMER_LEFT_43=722,20
PRIMER_RIGHT_43=1675,20
PRIMER_LEFT_43_TM=59.182
PRIMER_RIGHT_43_TM=57.863
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=954
PRIMER_PAIR_44_PENALTY=2.965337
PRIMER_LEFT_44_PENALTY=1.445265
PRIMER_RIGHT_44_PENALTY=1.520071
PRIMER_LEFT_44_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_44_SEQUENCE=CCGTGGGAAAAACTATGTGAG
PRIMER_LEFT_44=722,21
PRIMER_RIGHT_44=1628,21
PRIMER_LEFT_44_TM=60.445
PRIMER_RIGHT_44_TM=59.480
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=907
PRIMER_PAIR_45_PENALTY=2.970734
PRIMER_LEFT_45_PENALTY=1.445265
PRIMER_RIGHT_45_PENALTY=1.525468
PRIMER_LEFT_45_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_45_SEQUENCE=CCGTGGGAAAAACTATGTGA
PRIMER_LEFT_45=722,21
PRIMER_RIGHT_45=1628,20
PRIMER_LEFT_45_TM=60.445
PRIMER_RIGHT_45_TM=58.475
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=907
PRIMER_PAIR_46_PENALTY=2.975310
PRIMER_LEFT_46_PENALTY=0.818040
PRIMER_RIGHT_46_PENALTY=2.157269
PRIMER_LEFT_46_SEQUENCE=CCATGGCTCCTAATTTTGCT
PRIMER_RIGHT_46_SEQUENCE=GAAGCTGGAGAAGGAGCTTGA
PRIMER_LEFT_46=722,20
PRIMER_RIGHT_46=1433,21
PRIMER_LEFT_46_TM=59.182
PRIMER_RIGHT_46_TM=61.157
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=6.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=712
PRIMER_PAIR_47_PENALTY=3.016987
PRIMER_LEFT_47_PENALTY=1.445265
PRIMER_RIGHT_47_PENALTY=1.571721
PRIMER_LEFT_47_SEQUENCE=CCATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_47_SEQUENCE=GCTGGAGAAGGAGCTTGAGG
PRIMER_LEFT_47=722,21
PRIMER_RIGHT_47=1430,20
PRIMER_LEFT_47_TM=60.445
PRIMER_RIGHT_47_TM=61.572
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=60.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=709
PRIMER_PAIR_48_PENALTY=3.091988
PRIMER_LEFT_48_PENALTY=2.981596
PRIMER_RIGHT_48_PENALTY=0.110392
PRIMER_LEFT_48_SEQUENCE=CATGGCTCCTAATTTTGCTT
PRIMER_RIGHT_48_SEQUENCE=ATGAAAACGTCAGGCTTTGG
PRIMER_LEFT_48=723,20
PRIMER_RIGHT_48=1502,20
PRIMER_LEFT_48_TM=57.018
PRIMER_RIGHT_48_TM=60.110
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=8.8000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=780
PRIMER_PAIR_49_PENALTY=3.098154
PRIMER_LEFT_49_PENALTY=2.406591
PRIMER_RIGHT_49_PENALTY=0.691563
PRIMER_LEFT_49_SEQUENCE=TGGCTCCTAATTTTGCTTCTC
PRIMER_RIGHT_49_SEQUENCE=GAAGCTGGAGAAGGAGCTTG
PRIMER_LEFT_49=725,21
PRIMER_RIGHT_49=1433,20
PRIMER_LEFT_49_TM=58.593
PRIMER_RIGHT_49_TM=59.308
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=709
=
SEQUENCE_ID=MAGIC.149_5_20749950_20750020_wil-2_edi-0_91350_133454_TRUE
SEQUENCE_TARGET=950,170
SEQUENCE_TEMPLATE=ACCAATATGACACTTAGTTATNAGCNAGNNNACNANAAAAAANGATCGAATGAATCTCATATNNNNNNNNNCAAAAACTTATTNAAACGTCCAACAAAATTGTTTNCAGTTTNANATATCNATGNANNNCAGAATTTCATAATTGCATCTCNTGGANGATAAGCTTGTANCANGTAAACTAGTTTATATGNTTTGANTAGANCNAAANNTAATTCTTCCTANTGAGTNAGGGNTATATACAAACATTTATTATAANCAANATTTCATANANATGAANACATAAAANNTTNGAATNGGANNATTGATACCTCTGGTGATCAGANTAGGGAATAGTACGNAGGAGGGAGATCTCTTGGAGTCTCTTATCTCGTTTCTCTTTCGATTTTTCTTTGCTTCCTACCATCTTTCTNCTTATTTTCTCTCTTNNACGATCCNNNNNNNNNNNNNNNNNNNNNNNNNAGGAGGCAAGAAAGAACACAGAGCTTCGTCGTCGTTCTCATCTCCCTCTCACATAATTTTCTCATCCTTTTTGTGGGTCAAAAGGTGCAATCCNCCACTTTCTGCGTAATTTTTTTGTGGCCTCTCATTTCNAATTCATNTNATTCCTATTTTTAGTTATAGNATCTGTTATGGGAACATAAATTAGCTGGNNCCTAGCTAGGNTGTGGGCTCTCAATACGAACNAAAGTTTTGTTCATTCTGTTTTAGTTNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCGACCATTTGGTTTTGTGGCGGTTTAAAATTGNGAAAACAATNNTATTTAAGAGNCTCTCGTGTTNCACACTCTCACTANGANNCTATGATCCACATGACCACATGCATGTCACGTTGNATCCTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCATATGTGNTCANGAGAAGGCCACGACAAGTATAGGCCCATTAATGGGTCCANAAGTACCCACTTTCATTACAGAACAANACTTTNCTGCCGAAAAGAGAGAGNNNNAACAAAACAAAAAATGGCTACNCGAAAAGCTCTGATCAACGTTCACCGGATCATTCGCTCCACCGCCGTGGNTGGCCGGAGCTCNATCATTCCTGCCGNCGNTAACCGGNCATATCCTANTNTATTCCGGTACTTTAANNTTCTNTCCACNGTAATAATANTGATCTGTGTATTCGTGAACACGCCTGNNAAAAAAATTGTGGGATTGNTATTTTNTCTAGGAATGGAGTTGATTTGGGGGCGAGATTCTTCTGTGAGAACACTGCGTCTNCCCAGAATNTCGATATTGATCTCTCTANCGAAGANAATAAGAGAAGAACGATCAACAGGTAN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.488674
PRIMER_LEFT_0_PENALTY=0.585054
PRIMER_RIGHT_0_PENALTY=0.903619
PRIMER_LEFT_0_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_0_SEQUENCE=TGGGCCTATACTTGTCGTGG
PRIMER_LEFT_0=753,20
PRIMER_RIGHT_0=1669,20
PRIMER_LEFT_0_TM=59.415
PRIMER_RIGHT_0_TM=60.904
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=917
PRIMER_PAIR_1_PENALTY=2.101687
PRIMER_LEFT_1_PENALTY=0.585054
PRIMER_RIGHT_1_PENALTY=1.516632
PRIMER_LEFT_1_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_1_SEQUENCE=GCCTATACTTGTCGTGGCCTT
PRIMER_LEFT_1=753,20
PRIMER_RIGHT_1=1666,21
PRIMER_LEFT_1_TM=59.415
PRIMER_RIGHT_1_TM=60.517
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=914
PRIMER_PAIR_2_PENALTY=2.376402
PRIMER_LEFT_2_PENALTY=1.472783
PRIMER_RIGHT_2_PENALTY=0.903619
PRIMER_LEFT_2_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_2_SEQUENCE=TGGGCCTATACTTGTCGTGG
PRIMER_LEFT_2=760,20
PRIMER_RIGHT_2=1669,20
PRIMER_LEFT_2_TM=61.473
PRIMER_RIGHT_2_TM=60.904
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=910
PRIMER_PAIR_3_PENALTY=2.598736
PRIMER_LEFT_3_PENALTY=0.585054
PRIMER_RIGHT_3_PENALTY=2.013682
PRIMER_LEFT_3_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_3_SEQUENCE=AATGGGCCTATACTTGTCGTG
PRIMER_LEFT_3=753,20
PRIMER_RIGHT_3=1671,21
PRIMER_LEFT_3_TM=59.415
PRIMER_RIGHT_3_TM=58.986
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=919
PRIMER_PAIR_4_PENALTY=2.601254
PRIMER_LEFT_4_PENALTY=1.697634
PRIMER_RIGHT_4_PENALTY=0.903619
PRIMER_LEFT_4_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_4_SEQUENCE=TGGGCCTATACTTGTCGTGG
PRIMER_LEFT_4=754,20
PRIMER_RIGHT_4=1669,20
PRIMER_LEFT_4_TM=61.698
PRIMER_RIGHT_4_TM=60.904
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=916
PRIMER_PAIR_5_PENALTY=2.706350
PRIMER_LEFT_5_PENALTY=0.585054
PRIMER_RIGHT_5_PENALTY=2.121295
PRIMER_LEFT_5_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_5_SEQUENCE=ATGGGCCTATACTTGTCGTGG
PRIMER_LEFT_5=753,20
PRIMER_RIGHT_5=1670,21
PRIMER_LEFT_5_TM=59.415
PRIMER_RIGHT_5_TM=61.121
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=52.381
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=918
PRIMER_PAIR_6_PENALTY=2.853475
PRIMER_LEFT_6_PENALTY=0.585054
PRIMER_RIGHT_6_PENALTY=2.268421
PRIMER_LEFT_6_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_6_SEQUENCE=CCTATACTTGTCGTGGCCTTC
PRIMER_LEFT_6=753,20
PRIMER_RIGHT_6=1665,21
PRIMER_LEFT_6_TM=59.415
PRIMER_RIGHT_6_TM=58.732
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=913
PRIMER_PAIR_7_PENALTY=2.910787
PRIMER_LEFT_7_PENALTY=0.585054
PRIMER_RIGHT_7_PENALTY=2.325733
PRIMER_LEFT_7_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_7_SEQUENCE=CCTATACTTGTCGTGGCCTTCT
PRIMER_LEFT_7=753,20
PRIMER_RIGHT_7=1665,22
PRIMER_LEFT_7_TM=59.415
PRIMER_RIGHT_7_TM=59.674
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=913
PRIMER_PAIR_8_PENALTY=2.989416
PRIMER_LEFT_8_PENALTY=1.472783
PRIMER_RIGHT_8_PENALTY=1.516632
PRIMER_LEFT_8_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_8_SEQUENCE=GCCTATACTTGTCGTGGCCTT
PRIMER_LEFT_8=760,20
PRIMER_RIGHT_8=1666,21
PRIMER_LEFT_8_TM=61.473
PRIMER_RIGHT_8_TM=60.517
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=52.381
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=907
PRIMER_PAIR_9_PENALTY=3.009870
PRIMER_LEFT_9_PENALTY=0.585054
PRIMER_RIGHT_9_PENALTY=2.424816
PRIMER_LEFT_9_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_9_SEQUENCE=ATGGGCCTATACTTGTCGTG
PRIMER_LEFT_9=753,20
PRIMER_RIGHT_9=1670,20
PRIMER_LEFT_9_TM=59.415
PRIMER_RIGHT_9_TM=57.575
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=918
PRIMER_PAIR_10_PENALTY=3.214267
PRIMER_LEFT_10_PENALTY=1.697634
PRIMER_RIGHT_10_PENALTY=1.516632
PRIMER_LEFT_10_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_10_SEQUENCE=GCCTATACTTGTCGTGGCCTT
PRIMER_LEFT_10=754,20
PRIMER_RIGHT_10=1666,21
PRIMER_LEFT_10_TM=61.698
PRIMER_RIGHT_10_TM=60.517
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=52.381
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=913
PRIMER_PAIR_11_PENALTY=3.486465
PRIMER_LEFT_11_PENALTY=1.472783
PRIMER_RIGHT_11_PENALTY=2.013682
PRIMER_LEFT_11_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_11_SEQUENCE=AATGGGCCTATACTTGTCGTG
PRIMER_LEFT_11=760,20
PRIMER_RIGHT_11=1671,21
PRIMER_LEFT_11_TM=61.473
PRIMER_RIGHT_11_TM=58.986
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=912
PRIMER_PAIR_12_PENALTY=3.552470
PRIMER_LEFT_12_PENALTY=0.585054
PRIMER_RIGHT_12_PENALTY=2.967416
PRIMER_LEFT_12_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_12_SEQUENCE=TAATGGGCCTATACTTGTCGTG
PRIMER_LEFT_12=753,20
PRIMER_RIGHT_12=1672,22
PRIMER_LEFT_12_TM=59.415
PRIMER_RIGHT_12_TM=59.033
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=45.455
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=920
PRIMER_PAIR_13_PENALTY=3.594079
PRIMER_LEFT_13_PENALTY=1.472783
PRIMER_RIGHT_13_PENALTY=2.121295
PRIMER_LEFT_13_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_13_SEQUENCE=ATGGGCCTATACTTGTCGTGG
PRIMER_LEFT_13=760,20
PRIMER_RIGHT_13=1670,21
PRIMER_LEFT_13_TM=61.473
PRIMER_RIGHT_13_TM=61.121
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=911
PRIMER_PAIR_14_PENALTY=3.711317
PRIMER_LEFT_14_PENALTY=1.697634
PRIMER_RIGHT_14_PENALTY=2.013682
PRIMER_LEFT_14_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_14_SEQUENCE=AATGGGCCTATACTTGTCGTG
PRIMER_LEFT_14=754,20
PRIMER_RIGHT_14=1671,21
PRIMER_LEFT_14_TM=61.698
PRIMER_RIGHT_14_TM=58.986
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=918
PRIMER_PAIR_15_PENALTY=3.729725
PRIMER_LEFT_15_PENALTY=0.585054
PRIMER_RIGHT_15_PENALTY=3.144671
PRIMER_LEFT_15_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_15_SEQUENCE=ATACTTGTCGTGGCCTTCTC
PRIMER_LEFT_15=753,20
PRIMER_RIGHT_15=1662,20
PRIMER_LEFT_15_TM=59.415
PRIMER_RIGHT_15_TM=56.855
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=910
PRIMER_PAIR_16_PENALTY=3.732994
PRIMER_LEFT_16_PENALTY=0.585054
PRIMER_RIGHT_16_PENALTY=3.147940
PRIMER_LEFT_16_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_16_SEQUENCE=CCTATACTTGTCGTGGCCTT
PRIMER_LEFT_16=753,20
PRIMER_RIGHT_16=1665,20
PRIMER_LEFT_16_TM=59.415
PRIMER_RIGHT_16_TM=56.852
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=913
PRIMER_PAIR_17_PENALTY=3.741204
PRIMER_LEFT_17_PENALTY=1.472783
PRIMER_RIGHT_17_PENALTY=2.268421
PRIMER_LEFT_17_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_17_SEQUENCE=CCTATACTTGTCGTGGCCTTC
PRIMER_LEFT_17=760,20
PRIMER_RIGHT_17=1665,21
PRIMER_LEFT_17_TM=61.473
PRIMER_RIGHT_17_TM=58.732
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=906
PRIMER_PAIR_18_PENALTY=3.798516
PRIMER_LEFT_18_PENALTY=1.472783
PRIMER_RIGHT_18_PENALTY=2.325733
PRIMER_LEFT_18_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_18_SEQUENCE=CCTATACTTGTCGTGGCCTTCT
PRIMER_LEFT_18=760,20
PRIMER_RIGHT_18=1665,22
PRIMER_LEFT_18_TM=61.473
PRIMER_RIGHT_18_TM=59.674
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=906
PRIMER_PAIR_19_PENALTY=3.818930
PRIMER_LEFT_19_PENALTY=1.697634
PRIMER_RIGHT_19_PENALTY=2.121295
PRIMER_LEFT_19_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_19_SEQUENCE=ATGGGCCTATACTTGTCGTGG
PRIMER_LEFT_19=754,20
PRIMER_RIGHT_19=1670,21
PRIMER_LEFT_19_TM=61.698
PRIMER_RIGHT_19_TM=61.121
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=917
PRIMER_PAIR_20_PENALTY=3.821290
PRIMER_LEFT_20_PENALTY=2.917671
PRIMER_RIGHT_20_PENALTY=0.903619
PRIMER_LEFT_20_SEQUENCE=CATTTGGTTTTGTGGCGGTT
PRIMER_RIGHT_20_SEQUENCE=TGGGCCTATACTTGTCGTGG
PRIMER_LEFT_20=759,20
PRIMER_RIGHT_20=1669,20
PRIMER_LEFT_20_TM=62.918
PRIMER_RIGHT_20_TM=60.904
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.9000
PRIMER_RIGHT_20_END_STABILITY=9.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=911
PRIMER_PAIR_21_PENALTY=3.842439
PRIMER_LEFT_21_PENALTY=0.585054
PRIMER_RIGHT_21_PENALTY=3.257385
PRIMER_LEFT_21_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_21_SEQUENCE=TTAATGGGCCTATACTTGTCGTG
PRIMER_LEFT_21=753,20
PRIMER_RIGHT_21=1673,23
PRIMER_LEFT_21_TM=59.415
PRIMER_RIGHT_21_TM=60.257
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=43.478
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=8.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=921
PRIMER_PAIR_22_PENALTY=3.897599
PRIMER_LEFT_22_PENALTY=1.472783
PRIMER_RIGHT_22_PENALTY=2.424816
PRIMER_LEFT_22_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_22_SEQUENCE=ATGGGCCTATACTTGTCGTG
PRIMER_LEFT_22=760,20
PRIMER_RIGHT_22=1670,20
PRIMER_LEFT_22_TM=61.473
PRIMER_RIGHT_22_TM=57.575
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.4000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=911
PRIMER_PAIR_23_PENALTY=3.966056
PRIMER_LEFT_23_PENALTY=1.697634
PRIMER_RIGHT_23_PENALTY=2.268421
PRIMER_LEFT_23_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_23_SEQUENCE=CCTATACTTGTCGTGGCCTTC
PRIMER_LEFT_23=754,20
PRIMER_RIGHT_23=1665,21
PRIMER_LEFT_23_TM=61.698
PRIMER_RIGHT_23_TM=58.732
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=912
PRIMER_PAIR_24_PENALTY=4.023367
PRIMER_LEFT_24_PENALTY=1.697634
PRIMER_RIGHT_24_PENALTY=2.325733
PRIMER_LEFT_24_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_24_SEQUENCE=CCTATACTTGTCGTGGCCTTCT
PRIMER_LEFT_24=754,20
PRIMER_RIGHT_24=1665,22
PRIMER_LEFT_24_TM=61.698
PRIMER_RIGHT_24_TM=59.674
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=912
PRIMER_PAIR_25_PENALTY=4.122451
PRIMER_LEFT_25_PENALTY=1.697634
PRIMER_RIGHT_25_PENALTY=2.424816
PRIMER_LEFT_25_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_25_SEQUENCE=ATGGGCCTATACTTGTCGTG
PRIMER_LEFT_25=754,20
PRIMER_RIGHT_25=1670,20
PRIMER_LEFT_25_TM=61.698
PRIMER_RIGHT_25_TM=57.575
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=917
PRIMER_PAIR_26_PENALTY=4.386554
PRIMER_LEFT_26_PENALTY=0.585054
PRIMER_RIGHT_26_PENALTY=3.801500
PRIMER_LEFT_26_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_26_SEQUENCE=AATGGGCCTATACTTGTCGT
PRIMER_LEFT_26=753,20
PRIMER_RIGHT_26=1671,20
PRIMER_LEFT_26_TM=59.415
PRIMER_RIGHT_26_TM=56.199
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.8000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=919
PRIMER_PAIR_27_PENALTY=4.420681
PRIMER_LEFT_27_PENALTY=1.276010
PRIMER_RIGHT_27_PENALTY=3.144671
PRIMER_LEFT_27_SEQUENCE=TGTGGGCTCTCAATACGAAC
PRIMER_RIGHT_27_SEQUENCE=ATACTTGTCGTGGCCTTCTC
PRIMER_LEFT_27=663,20
PRIMER_RIGHT_27=1662,20
PRIMER_LEFT_27_TM=58.724
PRIMER_RIGHT_27_TM=56.855
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=1000
PRIMER_PAIR_28_PENALTY=4.434303
PRIMER_LEFT_28_PENALTY=2.917671
PRIMER_RIGHT_28_PENALTY=1.516632
PRIMER_LEFT_28_SEQUENCE=CATTTGGTTTTGTGGCGGTT
PRIMER_RIGHT_28_SEQUENCE=GCCTATACTTGTCGTGGCCTT
PRIMER_LEFT_28=759,20
PRIMER_RIGHT_28=1666,21
PRIMER_LEFT_28_TM=62.918
PRIMER_RIGHT_28_TM=60.517
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.9000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=908
PRIMER_PAIR_29_PENALTY=4.440199
PRIMER_LEFT_29_PENALTY=1.472783
PRIMER_RIGHT_29_PENALTY=2.967416
PRIMER_LEFT_29_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_29_SEQUENCE=TAATGGGCCTATACTTGTCGTG
PRIMER_LEFT_29=760,20
PRIMER_RIGHT_29=1672,22
PRIMER_LEFT_29_TM=61.473
PRIMER_RIGHT_29_TM=59.033
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=45.455
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=8.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=913
PRIMER_PAIR_30_PENALTY=4.550346
PRIMER_LEFT_30_PENALTY=0.585054
PRIMER_RIGHT_30_PENALTY=3.965292
PRIMER_LEFT_30_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_30_SEQUENCE=CTATACTTGTCGTGGCCTTCTC
PRIMER_LEFT_30=753,20
PRIMER_RIGHT_30=1664,22
PRIMER_LEFT_30_TM=59.415
PRIMER_RIGHT_30_TM=58.035
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=912
PRIMER_PAIR_31_PENALTY=4.598377
PRIMER_LEFT_31_PENALTY=0.585054
PRIMER_RIGHT_31_PENALTY=4.013323
PRIMER_LEFT_31_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_31_SEQUENCE=TATACTTGTCGTGGCCTTCTC
PRIMER_LEFT_31=753,20
PRIMER_RIGHT_31=1663,21
PRIMER_LEFT_31_TM=59.415
PRIMER_RIGHT_31_TM=56.987
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=911
PRIMER_PAIR_32_PENALTY=4.617454
PRIMER_LEFT_32_PENALTY=1.472783
PRIMER_RIGHT_32_PENALTY=3.144671
PRIMER_LEFT_32_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_32_SEQUENCE=ATACTTGTCGTGGCCTTCTC
PRIMER_LEFT_32=760,20
PRIMER_RIGHT_32=1662,20
PRIMER_LEFT_32_TM=61.473
PRIMER_RIGHT_32_TM=56.855
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=903
PRIMER_PAIR_33_PENALTY=4.620723
PRIMER_LEFT_33_PENALTY=1.472783
PRIMER_RIGHT_33_PENALTY=3.147940
PRIMER_LEFT_33_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_33_SEQUENCE=CCTATACTTGTCGTGGCCTT
PRIMER_LEFT_33=760,20
PRIMER_RIGHT_33=1665,20
PRIMER_LEFT_33_TM=61.473
PRIMER_RIGHT_33_TM=56.852
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=9.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=906
PRIMER_PAIR_34_PENALTY=4.665050
PRIMER_LEFT_34_PENALTY=1.697634
PRIMER_RIGHT_34_PENALTY=2.967416
PRIMER_LEFT_34_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_34_SEQUENCE=TAATGGGCCTATACTTGTCGTG
PRIMER_LEFT_34=754,20
PRIMER_RIGHT_34=1672,22
PRIMER_LEFT_34_TM=61.698
PRIMER_RIGHT_34_TM=59.033
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=919
PRIMER_PAIR_35_PENALTY=4.730168
PRIMER_LEFT_35_PENALTY=1.472783
PRIMER_RIGHT_35_PENALTY=3.257385
PRIMER_LEFT_35_SEQUENCE=ATTTGGTTTTGTGGCGGTTT
PRIMER_RIGHT_35_SEQUENCE=TTAATGGGCCTATACTTGTCGTG
PRIMER_LEFT_35=760,20
PRIMER_RIGHT_35=1673,23
PRIMER_LEFT_35_TM=61.473
PRIMER_RIGHT_35_TM=60.257
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=43.478
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=914
PRIMER_PAIR_36_PENALTY=4.773918
PRIMER_LEFT_36_PENALTY=0.585054
PRIMER_RIGHT_36_PENALTY=4.188864
PRIMER_LEFT_36_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_36_SEQUENCE=GCCTATACTTGTCGTGGCCTTC
PRIMER_LEFT_36=753,20
PRIMER_RIGHT_36=1666,22
PRIMER_LEFT_36_TM=59.415
PRIMER_RIGHT_36_TM=62.189
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=54.545
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=914
PRIMER_PAIR_37_PENALTY=4.805175
PRIMER_LEFT_37_PENALTY=3.901556
PRIMER_RIGHT_37_PENALTY=0.903619
PRIMER_LEFT_37_SEQUENCE=TTCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_37_SEQUENCE=TGGGCCTATACTTGTCGTGG
PRIMER_LEFT_37=753,21
PRIMER_RIGHT_37=1669,20
PRIMER_LEFT_37_TM=62.902
PRIMER_RIGHT_37_TM=60.904
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=917
PRIMER_PAIR_38_PENALTY=4.842305
PRIMER_LEFT_38_PENALTY=1.697634
PRIMER_RIGHT_38_PENALTY=3.144671
PRIMER_LEFT_38_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_38_SEQUENCE=ATACTTGTCGTGGCCTTCTC
PRIMER_LEFT_38=754,20
PRIMER_RIGHT_38=1662,20
PRIMER_LEFT_38_TM=61.698
PRIMER_RIGHT_38_TM=56.855
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=909
PRIMER_PAIR_39_PENALTY=4.845574
PRIMER_LEFT_39_PENALTY=1.697634
PRIMER_RIGHT_39_PENALTY=3.147940
PRIMER_LEFT_39_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_39_SEQUENCE=CCTATACTTGTCGTGGCCTT
PRIMER_LEFT_39=754,20
PRIMER_RIGHT_39=1665,20
PRIMER_LEFT_39_TM=61.698
PRIMER_RIGHT_39_TM=56.852
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=9.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=912
PRIMER_PAIR_40_PENALTY=4.846337
PRIMER_LEFT_40_PENALTY=0.585054
PRIMER_RIGHT_40_PENALTY=4.261283
PRIMER_LEFT_40_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_40_SEQUENCE=TTAATGGGCCTATACTTGTCGT
PRIMER_LEFT_40=753,20
PRIMER_RIGHT_40=1673,22
PRIMER_LEFT_40_TM=59.415
PRIMER_RIGHT_40_TM=57.739
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.8000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=921
PRIMER_PAIR_41_PENALTY=4.863648
PRIMER_LEFT_41_PENALTY=0.585054
PRIMER_RIGHT_41_PENALTY=4.278594
PRIMER_LEFT_41_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_41_SEQUENCE=AATGGGCCTATACTTGTCGTGG
PRIMER_LEFT_41=753,20
PRIMER_RIGHT_41=1671,22
PRIMER_LEFT_41_TM=59.415
PRIMER_RIGHT_41_TM=62.279
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=9.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=919
PRIMER_PAIR_42_PENALTY=4.928815
PRIMER_LEFT_42_PENALTY=0.585054
PRIMER_RIGHT_42_PENALTY=4.343761
PRIMER_LEFT_42_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_42_SEQUENCE=CCTATACTTGTCGTGGCCTTCTC
PRIMER_LEFT_42=753,20
PRIMER_RIGHT_42=1665,23
PRIMER_LEFT_42_TM=59.415
PRIMER_RIGHT_42_TM=61.344
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=52.174
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=913
PRIMER_PAIR_43_PENALTY=4.931353
PRIMER_LEFT_43_PENALTY=2.917671
PRIMER_RIGHT_43_PENALTY=2.013682
PRIMER_LEFT_43_SEQUENCE=CATTTGGTTTTGTGGCGGTT
PRIMER_RIGHT_43_SEQUENCE=AATGGGCCTATACTTGTCGTG
PRIMER_LEFT_43=759,20
PRIMER_RIGHT_43=1671,21
PRIMER_LEFT_43_TM=62.918
PRIMER_RIGHT_43_TM=58.986
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=8.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=913
PRIMER_PAIR_44_PENALTY=4.955020
PRIMER_LEFT_44_PENALTY=1.697634
PRIMER_RIGHT_44_PENALTY=3.257385
PRIMER_LEFT_44_SEQUENCE=TCGACCATTTGGTTTTGTGG
PRIMER_RIGHT_44_SEQUENCE=TTAATGGGCCTATACTTGTCGTG
PRIMER_LEFT_44=754,20
PRIMER_RIGHT_44=1673,23
PRIMER_LEFT_44_TM=61.698
PRIMER_RIGHT_44_TM=60.257
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=43.478
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=920
PRIMER_PAIR_45_PENALTY=5.038966
PRIMER_LEFT_45_PENALTY=2.917671
PRIMER_RIGHT_45_PENALTY=2.121295
PRIMER_LEFT_45_SEQUENCE=CATTTGGTTTTGTGGCGGTT
PRIMER_RIGHT_45_SEQUENCE=ATGGGCCTATACTTGTCGTGG
PRIMER_LEFT_45=759,20
PRIMER_RIGHT_45=1670,21
PRIMER_LEFT_45_TM=62.918
PRIMER_RIGHT_45_TM=61.121
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.9000
PRIMER_RIGHT_45_END_STABILITY=9.9000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=912
PRIMER_PAIR_46_PENALTY=5.063771
PRIMER_LEFT_46_PENALTY=0.585054
PRIMER_RIGHT_46_PENALTY=4.478716
PRIMER_LEFT_46_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_46_SEQUENCE=ATTAATGGGCCTATACTTGTCGTG
PRIMER_LEFT_46=753,20
PRIMER_RIGHT_46=1674,24
PRIMER_LEFT_46_TM=59.415
PRIMER_RIGHT_46_TM=60.479
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=41.667
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=922
PRIMER_PAIR_47_PENALTY=5.186092
PRIMER_LEFT_47_PENALTY=2.917671
PRIMER_RIGHT_47_PENALTY=2.268421
PRIMER_LEFT_47_SEQUENCE=CATTTGGTTTTGTGGCGGTT
PRIMER_RIGHT_47_SEQUENCE=CCTATACTTGTCGTGGCCTTC
PRIMER_LEFT_47=759,20
PRIMER_RIGHT_47=1665,21
PRIMER_LEFT_47_TM=62.918
PRIMER_RIGHT_47_TM=58.732
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=907
PRIMER_PAIR_48_PENALTY=5.236694
PRIMER_LEFT_48_PENALTY=0.585054
PRIMER_RIGHT_48_PENALTY=4.651639
PRIMER_LEFT_48_SEQUENCE=TTCGACCATTTGGTTTTGTG
PRIMER_RIGHT_48_SEQUENCE=TAATGGGCCTATACTTGTCGT
PRIMER_LEFT_48=753,20
PRIMER_RIGHT_48=1672,21
PRIMER_LEFT_48_TM=59.415
PRIMER_RIGHT_48_TM=56.348
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.8000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=920
PRIMER_PAIR_49_PENALTY=5.243404
PRIMER_LEFT_49_PENALTY=2.917671
PRIMER_RIGHT_49_PENALTY=2.325733
PRIMER_LEFT_49_SEQUENCE=CATTTGGTTTTGTGGCGGTT
PRIMER_RIGHT_49_SEQUENCE=CCTATACTTGTCGTGGCCTTCT
PRIMER_LEFT_49=759,20
PRIMER_RIGHT_49=1665,22
PRIMER_LEFT_49_TM=62.918
PRIMER_RIGHT_49_TM=59.674
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=907
=
SEQUENCE_ID=MAGIC.105_3_14642060_14642197_oy-0_ct-1_9323_29913_TRUE
SEQUENCE_TARGET=950,237
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNTNTNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_4712351_4712649_rsch-4_wu-0_87063_9861_TRUE
SEQUENCE_TARGET=950,398
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_528685_528892_rsch-4_po-0_157649_186200_TRUE
SEQUENCE_TARGET=950,307
SEQUENCE_TEMPLATE=TTTCGTAAGCAATGGTGAAGAAGTTGAGGAAGAGAGGATTGAACATTGCGTTTCTTTTCTNTTAGGTCTTTGAATTTTCTTTTATGAAGTCTCTAATACAAAACAATCTTNCTTANTCTGACAGNANTTATAAGGAATTGCTAGCCAAGTGCAATCAGCTGGGNAGAGGCGNAGCACNATCTTCTGAGAAACTTGAAAAAGCTTTGGAAAAGATAGAAAAACTAAAGGTGTGTGTTTTGGTGTTTCTCTCGATCTTCTTTGTGTCTTCTATATCTGGCTCTTATTATGTTTATTCTATGACCAGAAACGAATGAGGGAACTTGAGTTGATAACTGAAGAGAACAGAGCTNTACGGGACATAAACGTTTCAAAGAANTGCAGTTACACAGAAGTTTCCCTTAAAAGACCCGACATCGTCAATGTCACCTTACAGCAATGGTACTTTTGTTNTACATCATGAATTCAAGTTCTCTATAAAAACATTTGGTTANGNGTGTGTGTGTAGGAGTCATCTCNAATTATTTTTGAAAGGTTATCGTCTCTAATTATAAGTAAACTTCAAAACTAAGTGATGAGTTTTTGCCTCTTTCCAAAGTTATTTCCCAATGTATTGGTTGAATAGAACAGAAAGTATTCTACTGAAACATGTTTTGAAACGCNATTCCAAGACATCAATAACATAAATTACCACTTAACATAAACGAAGGGGTCTTATACCCAATCAAAGCATCCAACAATAGAAGCAAACAGAATAATTATCTGATAGAACAAAAGTCTTAAGAANCCAAACCACCGNTAAGAAACTAAAGCCGCAAAATGCAAAAANCAAACTAAAAAAGGATAAGAAAAACAAGCCAACAACAAANCACCNGCTAGCNCTTNCNTCTACTTACNGGAANAAGATGCGTTGTCTTGCTTAGACAGCNCATTCGTCACGGCAGCANCAAATTCACTTCTCATCATAGAGAGCTCCTCTTCCTTAGCCTGAATCTTCTTCAAGNTTTTTTCCNTCTTTCTCTTTTNTTTTCCNTCTTTGATCTTATCCAATTTTNCACTCCNTTTTGTGTCATCAACACCCAAAGAATTCGGATTCTCAACCNTCAAGTAAGAGACCAACTCTTGACCAGAAGATAAAGTGCTTATGACAAACCGATGTTTCTGTGGCTCTTGATCATCTTCATCAACCTTGACACTTCCTCCAAGCTGTNCTTGTCGCATNTTCTTTGATTTCGAATACAATACACCGTTACTTCCCCAAATCTTTCTCGACAAGTCAAAACAATTTTGGTCGGAAGCTTTCGAAAACACCATCNCTTCTTCATTCTTTCCTTTTTTAAGACCCTTCGAAACTCTGCCATTGAATTTTTTCTTCAAGTTNCCAANTTTGGTTACCANTTGAGGTTTGGTAACATCAAAACTGATGGAGTTCTTGATAGACTCATAAAAATCATCGATATGATCACTANGGTTTNCTTTCTTAGTAGCGAAATCAAGGAAGCCTTGNAGCAAAATAGCTTCGTCTTCTTCGGTGAATAACNTAACAAACAGATTCTTCTTTNGCGTTTCATCCTTCTTCTTCACATCCTCNNNNNNNNNNNNNTTCTTCGATTTCTTCTTACTCATTTCGTCTGTTTTCTCAAACGATCTCTTGGCAGCAGTAGAGCTTTCCGGTTTGGTAGTAGTCTTGGAACCAGAAGGCTTGNTNACAACCACCGGCTTCTTGGNAGACTCTTTGACGGGTGAANCGGCNGAAGAGTCAGACTCTTCACCTGAAGTAGCAGACTCGCCAGAAGAAGCAGACTCTTCTTCTTCAGATGANGACACTACTGGATTCTCGATTTTTTTCAACTGCTTTGNTGCCATTGTCAATCTAAAGACTATATCGATTTGGTTTNGAGGTTTTAAAAAGTTTGTTTGTGAAATGANAGTGGCGATTAACTAAGTGCGAGTATTTATAACTGGTTTAATAGTTGTTTNCTCTTGAGGAAGGTCAAGAGATACTTAATCATAGNCNTTTAAAAATATTACATTTTCCAAGTTATAAATGCNGCAGACATCAACTCTGTTTTCCAATACTCAATTTTACTTTTTCTTGAGAAAGCAACTATTACGGCTCATTGTACAAGTGACGAGGAAACTAATTATTGCTTGAACAAAAACAAATAATGTTTGGTCTTCCNGGAAGCTTTCCTCAGAGTATGGAACCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.821963
PRIMER_LEFT_0_PENALTY=0.481956
PRIMER_RIGHT_0_PENALTY=0.340007
PRIMER_LEFT_0_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_0_SEQUENCE=AAGGCTTCCTTGATTTCGCT
PRIMER_LEFT_0=717,20
PRIMER_RIGHT_0=1501,20
PRIMER_LEFT_0_TM=59.518
PRIMER_RIGHT_0_TM=60.340
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=9.9000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=785
PRIMER_PAIR_1_PENALTY=1.221903
PRIMER_LEFT_1_PENALTY=0.203367
PRIMER_RIGHT_1_PENALTY=1.018537
PRIMER_LEFT_1_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_1_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_1=715,20
PRIMER_RIGHT_1=1357,20
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=61.019
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=643
PRIMER_PAIR_2_PENALTY=1.272921
PRIMER_LEFT_2_PENALTY=0.203367
PRIMER_RIGHT_2_PENALTY=1.069554
PRIMER_LEFT_2_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_2_SEQUENCE=GGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_2=715,20
PRIMER_RIGHT_2=1499,20
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=58.930
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=785
PRIMER_PAIR_3_PENALTY=1.322090
PRIMER_LEFT_3_PENALTY=1.212889
PRIMER_RIGHT_3_PENALTY=0.109200
PRIMER_LEFT_3_SEQUENCE=CTAAAGCCGCAAAATGCAAA
PRIMER_RIGHT_3_SEQUENCE=CTTCTTCTGGCGAGTCTGCT
PRIMER_LEFT_3=803,20
PRIMER_RIGHT_3=1795,20
PRIMER_LEFT_3_TM=61.213
PRIMER_RIGHT_3_TM=59.891
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=993
PRIMER_PAIR_4_PENALTY=1.333404
PRIMER_LEFT_4_PENALTY=0.203367
PRIMER_RIGHT_4_PENALTY=1.130037
PRIMER_LEFT_4_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_4_SEQUENCE=GGCTTCCTTGATTTCGCTACT
PRIMER_LEFT_4=715,20
PRIMER_RIGHT_4=1499,21
PRIMER_LEFT_4_TM=59.797
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=785
PRIMER_PAIR_5_PENALTY=1.333404
PRIMER_LEFT_5_PENALTY=0.203367
PRIMER_RIGHT_5_PENALTY=1.130037
PRIMER_LEFT_5_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_5_SEQUENCE=AGGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_5=715,20
PRIMER_RIGHT_5=1500,21
PRIMER_LEFT_5_TM=59.797
PRIMER_RIGHT_5_TM=59.870
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=786
PRIMER_PAIR_6_PENALTY=1.369177
PRIMER_LEFT_6_PENALTY=1.212889
PRIMER_RIGHT_6_PENALTY=0.156287
PRIMER_LEFT_6_SEQUENCE=CTAAAGCCGCAAAATGCAAA
PRIMER_RIGHT_6_SEQUENCE=GGCGAGTCTGCTACTTCAGG
PRIMER_LEFT_6=803,20
PRIMER_RIGHT_6=1787,20
PRIMER_LEFT_6_TM=61.213
PRIMER_RIGHT_6_TM=60.156
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.8000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=985
PRIMER_PAIR_7_PENALTY=1.374697
PRIMER_LEFT_7_PENALTY=1.034689
PRIMER_RIGHT_7_PENALTY=0.340007
PRIMER_LEFT_7_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_7_SEQUENCE=AAGGCTTCCTTGATTTCGCT
PRIMER_LEFT_7=714,20
PRIMER_RIGHT_7=1501,20
PRIMER_LEFT_7_TM=58.965
PRIMER_RIGHT_7_TM=60.340
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.9000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=788
PRIMER_PAIR_8_PENALTY=1.500493
PRIMER_LEFT_8_PENALTY=0.481956
PRIMER_RIGHT_8_PENALTY=1.018537
PRIMER_LEFT_8_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_8_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_8=717,20
PRIMER_RIGHT_8=1357,20
PRIMER_LEFT_8_TM=59.518
PRIMER_RIGHT_8_TM=61.019
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=9.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=641
PRIMER_PAIR_9_PENALTY=1.513709
PRIMER_LEFT_9_PENALTY=0.495173
PRIMER_RIGHT_9_PENALTY=1.018537
PRIMER_LEFT_9_SEQUENCE=AAAAGACCCGACATCGTCAA
PRIMER_RIGHT_9_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_9=400,20
PRIMER_RIGHT_9=1357,20
PRIMER_LEFT_9_TM=60.495
PRIMER_RIGHT_9_TM=61.019
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=9.1000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=958
PRIMER_PAIR_10_PENALTY=1.551510
PRIMER_LEFT_10_PENALTY=0.481956
PRIMER_RIGHT_10_PENALTY=1.069554
PRIMER_LEFT_10_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_10_SEQUENCE=GGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_10=717,20
PRIMER_RIGHT_10=1499,20
PRIMER_LEFT_10_TM=59.518
PRIMER_RIGHT_10_TM=58.930
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=7.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=783
PRIMER_PAIR_11_PENALTY=1.611993
PRIMER_LEFT_11_PENALTY=0.481956
PRIMER_RIGHT_11_PENALTY=1.130037
PRIMER_LEFT_11_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_11_SEQUENCE=GGCTTCCTTGATTTCGCTACT
PRIMER_LEFT_11=717,20
PRIMER_RIGHT_11=1499,21
PRIMER_LEFT_11_TM=59.518
PRIMER_RIGHT_11_TM=59.870
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=5.4000
PRIMER_PAIR_11_COMPL_ANY=7.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=783
PRIMER_PAIR_12_PENALTY=1.611993
PRIMER_LEFT_12_PENALTY=0.481956
PRIMER_RIGHT_12_PENALTY=1.130037
PRIMER_LEFT_12_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_12_SEQUENCE=AGGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_12=717,20
PRIMER_RIGHT_12=1500,21
PRIMER_LEFT_12_TM=59.518
PRIMER_RIGHT_12_TM=59.870
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=784
PRIMER_PAIR_13_PENALTY=1.705781
PRIMER_LEFT_13_PENALTY=0.203367
PRIMER_RIGHT_13_PENALTY=1.502415
PRIMER_LEFT_13_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_13_SEQUENCE=AATGGCAGAGTTTCGAAGGG
PRIMER_LEFT_13=715,20
PRIMER_RIGHT_13=1358,20
PRIMER_LEFT_13_TM=59.797
PRIMER_RIGHT_13_TM=61.502
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=644
PRIMER_PAIR_14_PENALTY=1.788174
PRIMER_LEFT_14_PENALTY=0.203367
PRIMER_RIGHT_14_PENALTY=1.584808
PRIMER_LEFT_14_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_14_SEQUENCE=AAAGCTCTACTGCTGCCAAGA
PRIMER_LEFT_14=715,20
PRIMER_RIGHT_14=1666,21
PRIMER_LEFT_14_TM=59.797
PRIMER_RIGHT_14_TM=59.415
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=952
PRIMER_PAIR_15_PENALTY=1.788174
PRIMER_LEFT_15_PENALTY=0.203367
PRIMER_RIGHT_15_PENALTY=1.584808
PRIMER_LEFT_15_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_15_SEQUENCE=GAAAGCTCTACTGCTGCCAAG
PRIMER_LEFT_15=715,20
PRIMER_RIGHT_15=1667,21
PRIMER_LEFT_15_TM=59.797
PRIMER_RIGHT_15_TM=59.415
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=953
PRIMER_PAIR_16_PENALTY=1.797174
PRIMER_LEFT_16_PENALTY=0.203367
PRIMER_RIGHT_16_PENALTY=1.593807
PRIMER_LEFT_16_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_16_SEQUENCE=GAAAGCTCTACTGCTGCCAA
PRIMER_LEFT_16=715,20
PRIMER_RIGHT_16=1667,20
PRIMER_LEFT_16_TM=59.797
PRIMER_RIGHT_16_TM=58.406
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=10.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=953
PRIMER_PAIR_17_PENALTY=1.865475
PRIMER_LEFT_17_PENALTY=1.525468
PRIMER_RIGHT_17_PENALTY=0.340007
PRIMER_LEFT_17_SEQUENCE=TGAGTTTTTGCCTCTTTCCA
PRIMER_RIGHT_17_SEQUENCE=AAGGCTTCCTTGATTTCGCT
PRIMER_LEFT_17=572,20
PRIMER_RIGHT_17=1501,20
PRIMER_LEFT_17_TM=58.475
PRIMER_RIGHT_17_TM=60.340
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=930
PRIMER_PAIR_18_PENALTY=1.873670
PRIMER_LEFT_18_PENALTY=1.212889
PRIMER_RIGHT_18_PENALTY=0.660780
PRIMER_LEFT_18_SEQUENCE=CTAAAGCCGCAAAATGCAAA
PRIMER_RIGHT_18_SEQUENCE=CTGGCGAGTCTGCTACTTCA
PRIMER_LEFT_18=803,20
PRIMER_RIGHT_18=1789,20
PRIMER_LEFT_18_TM=61.213
PRIMER_RIGHT_18_TM=59.339
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.8000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=987
PRIMER_PAIR_19_PENALTY=1.984371
PRIMER_LEFT_19_PENALTY=0.481956
PRIMER_RIGHT_19_PENALTY=1.502415
PRIMER_LEFT_19_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_19_SEQUENCE=AATGGCAGAGTTTCGAAGGG
PRIMER_LEFT_19=717,20
PRIMER_RIGHT_19=1358,20
PRIMER_LEFT_19_TM=59.518
PRIMER_RIGHT_19_TM=61.502
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=642
PRIMER_PAIR_20_PENALTY=1.997587
PRIMER_LEFT_20_PENALTY=0.495173
PRIMER_RIGHT_20_PENALTY=1.502415
PRIMER_LEFT_20_SEQUENCE=AAAAGACCCGACATCGTCAA
PRIMER_RIGHT_20_SEQUENCE=AATGGCAGAGTTTCGAAGGG
PRIMER_LEFT_20=400,20
PRIMER_RIGHT_20=1358,20
PRIMER_LEFT_20_TM=60.495
PRIMER_RIGHT_20_TM=61.502
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.7000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=959
PRIMER_PAIR_21_PENALTY=2.053226
PRIMER_LEFT_21_PENALTY=1.034689
PRIMER_RIGHT_21_PENALTY=1.018537
PRIMER_LEFT_21_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_21_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_21=714,20
PRIMER_RIGHT_21=1357,20
PRIMER_LEFT_21_TM=58.965
PRIMER_RIGHT_21_TM=61.019
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=9.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=644
PRIMER_PAIR_22_PENALTY=2.066764
PRIMER_LEFT_22_PENALTY=0.481956
PRIMER_RIGHT_22_PENALTY=1.584808
PRIMER_LEFT_22_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_22_SEQUENCE=AAAGCTCTACTGCTGCCAAGA
PRIMER_LEFT_22=717,20
PRIMER_RIGHT_22=1666,21
PRIMER_LEFT_22_TM=59.518
PRIMER_RIGHT_22_TM=59.415
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=950
PRIMER_PAIR_23_PENALTY=2.066764
PRIMER_LEFT_23_PENALTY=0.481956
PRIMER_RIGHT_23_PENALTY=1.584808
PRIMER_LEFT_23_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_23_SEQUENCE=GAAAGCTCTACTGCTGCCAAG
PRIMER_LEFT_23=717,20
PRIMER_RIGHT_23=1667,21
PRIMER_LEFT_23_TM=59.518
PRIMER_RIGHT_23_TM=59.415
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=951
PRIMER_PAIR_24_PENALTY=2.075763
PRIMER_LEFT_24_PENALTY=0.481956
PRIMER_RIGHT_24_PENALTY=1.593807
PRIMER_LEFT_24_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_24_SEQUENCE=GAAAGCTCTACTGCTGCCAA
PRIMER_LEFT_24=717,20
PRIMER_RIGHT_24=1667,20
PRIMER_LEFT_24_TM=59.518
PRIMER_RIGHT_24_TM=58.406
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=10.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=951
PRIMER_PAIR_25_PENALTY=2.104244
PRIMER_LEFT_25_PENALTY=1.034689
PRIMER_RIGHT_25_PENALTY=1.069554
PRIMER_LEFT_25_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_25_SEQUENCE=GGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_25=714,20
PRIMER_RIGHT_25=1499,20
PRIMER_LEFT_25_TM=58.965
PRIMER_RIGHT_25_TM=58.930
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=7.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=786
PRIMER_PAIR_26_PENALTY=2.151342
PRIMER_LEFT_26_PENALTY=1.811335
PRIMER_RIGHT_26_PENALTY=0.340007
PRIMER_LEFT_26_SEQUENCE=CCCAATCAAAGCATCCAACA
PRIMER_RIGHT_26_SEQUENCE=AAGGCTTCCTTGATTTCGCT
PRIMER_LEFT_26=716,20
PRIMER_RIGHT_26=1501,20
PRIMER_LEFT_26_TM=61.811
PRIMER_RIGHT_26_TM=60.340
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=786
PRIMER_PAIR_27_PENALTY=2.164727
PRIMER_LEFT_27_PENALTY=1.034689
PRIMER_RIGHT_27_PENALTY=1.130037
PRIMER_LEFT_27_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_27_SEQUENCE=GGCTTCCTTGATTTCGCTACT
PRIMER_LEFT_27=714,20
PRIMER_RIGHT_27=1499,21
PRIMER_LEFT_27_TM=58.965
PRIMER_RIGHT_27_TM=59.870
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=47.619
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=5.4000
PRIMER_PAIR_27_COMPL_ANY=7.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=786
PRIMER_PAIR_28_PENALTY=2.164727
PRIMER_LEFT_28_PENALTY=1.034689
PRIMER_RIGHT_28_PENALTY=1.130037
PRIMER_LEFT_28_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_28_SEQUENCE=AGGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_28=714,20
PRIMER_RIGHT_28=1500,21
PRIMER_LEFT_28_TM=58.965
PRIMER_RIGHT_28_TM=59.870
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=6.9000
PRIMER_PAIR_28_COMPL_ANY=7.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=787
PRIMER_PAIR_29_PENALTY=2.184105
PRIMER_LEFT_29_PENALTY=0.203367
PRIMER_RIGHT_29_PENALTY=1.980738
PRIMER_LEFT_29_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_29_SEQUENCE=GCCAAGAGATCGTTTGAGAA
PRIMER_LEFT_29=715,20
PRIMER_RIGHT_29=1652,20
PRIMER_LEFT_29_TM=59.797
PRIMER_RIGHT_29_TM=58.019
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=938
PRIMER_PAIR_30_PENALTY=2.195687
PRIMER_LEFT_30_PENALTY=0.203367
PRIMER_RIGHT_30_PENALTY=1.992321
PRIMER_LEFT_30_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_30_SEQUENCE=AAGCTCTACTGCTGCCAAGA
PRIMER_LEFT_30=715,20
PRIMER_RIGHT_30=1665,20
PRIMER_LEFT_30_TM=59.797
PRIMER_RIGHT_30_TM=58.008
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=951
PRIMER_PAIR_31_PENALTY=2.203582
PRIMER_LEFT_31_PENALTY=1.185045
PRIMER_RIGHT_31_PENALTY=1.018537
PRIMER_LEFT_31_SEQUENCE=TACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_31_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_31=714,21
PRIMER_RIGHT_31=1357,20
PRIMER_LEFT_31_TM=59.815
PRIMER_RIGHT_31_TM=61.019
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=9.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=644
PRIMER_PAIR_32_PENALTY=2.231426
PRIMER_LEFT_32_PENALTY=1.212889
PRIMER_RIGHT_32_PENALTY=1.018537
PRIMER_LEFT_32_SEQUENCE=CTAAAGCCGCAAAATGCAAA
PRIMER_RIGHT_32_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_32=803,20
PRIMER_RIGHT_32=1357,20
PRIMER_LEFT_32_TM=61.213
PRIMER_RIGHT_32_TM=61.019
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.8000
PRIMER_RIGHT_32_END_STABILITY=9.1000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=555
PRIMER_PAIR_33_PENALTY=2.245810
PRIMER_LEFT_33_PENALTY=1.227274
PRIMER_RIGHT_33_PENALTY=1.018537
PRIMER_LEFT_33_SEQUENCE=CGTCAATGTCACCTTACAGCA
PRIMER_RIGHT_33_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_33=414,21
PRIMER_RIGHT_33=1357,20
PRIMER_LEFT_33_TM=59.773
PRIMER_RIGHT_33_TM=61.019
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=9.1000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=944
PRIMER_PAIR_34_PENALTY=2.290397
PRIMER_LEFT_34_PENALTY=0.203367
PRIMER_RIGHT_34_PENALTY=2.087031
PRIMER_LEFT_34_SEQUENCE=ACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_34_SEQUENCE=CACCGAAGAAGACGAAGCTATT
PRIMER_LEFT_34=715,20
PRIMER_RIGHT_34=1530,22
PRIMER_LEFT_34_TM=59.797
PRIMER_RIGHT_34_TM=59.913
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=5.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=816
PRIMER_PAIR_35_PENALTY=2.315082
PRIMER_LEFT_35_PENALTY=1.185045
PRIMER_RIGHT_35_PENALTY=1.130037
PRIMER_LEFT_35_SEQUENCE=TACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_35_SEQUENCE=GGCTTCCTTGATTTCGCTACT
PRIMER_LEFT_35=714,21
PRIMER_RIGHT_35=1499,21
PRIMER_LEFT_35_TM=59.815
PRIMER_RIGHT_35_TM=59.870
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=5.4000
PRIMER_PAIR_35_COMPL_ANY=7.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=786
PRIMER_PAIR_36_PENALTY=2.315082
PRIMER_LEFT_36_PENALTY=1.185045
PRIMER_RIGHT_36_PENALTY=1.130037
PRIMER_LEFT_36_SEQUENCE=TACCCAATCAAAGCATCCAAC
PRIMER_RIGHT_36_SEQUENCE=AGGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_36=714,21
PRIMER_RIGHT_36=1500,21
PRIMER_LEFT_36_TM=59.815
PRIMER_RIGHT_36_TM=59.870
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=7.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=787
PRIMER_PAIR_37_PENALTY=2.441133
PRIMER_LEFT_37_PENALTY=1.212889
PRIMER_RIGHT_37_PENALTY=1.228243
PRIMER_LEFT_37_SEQUENCE=CTAAAGCCGCAAAATGCAAA
PRIMER_RIGHT_37_SEQUENCE=TTCTGGCGAGTCTGCTACTTC
PRIMER_LEFT_37=803,20
PRIMER_RIGHT_37=1791,21
PRIMER_LEFT_37_TM=61.213
PRIMER_RIGHT_37_TM=59.772
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=52.381
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.8000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=989
PRIMER_PAIR_38_PENALTY=2.441133
PRIMER_LEFT_38_PENALTY=1.212889
PRIMER_RIGHT_38_PENALTY=1.228243
PRIMER_LEFT_38_SEQUENCE=CTAAAGCCGCAAAATGCAAA
PRIMER_RIGHT_38_SEQUENCE=TTCTTCTGGCGAGTCTGCTAC
PRIMER_LEFT_38=803,20
PRIMER_RIGHT_38=1794,21
PRIMER_LEFT_38_TM=61.213
PRIMER_RIGHT_38_TM=59.772
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.8000
PRIMER_RIGHT_38_END_STABILITY=6.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=992
PRIMER_PAIR_39_PENALTY=2.462694
PRIMER_LEFT_39_PENALTY=0.481956
PRIMER_RIGHT_39_PENALTY=1.980738
PRIMER_LEFT_39_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_39_SEQUENCE=GCCAAGAGATCGTTTGAGAA
PRIMER_LEFT_39=717,20
PRIMER_RIGHT_39=1652,20
PRIMER_LEFT_39_TM=59.518
PRIMER_RIGHT_39_TM=58.019
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=936
PRIMER_PAIR_40_PENALTY=2.474277
PRIMER_LEFT_40_PENALTY=0.481956
PRIMER_RIGHT_40_PENALTY=1.992321
PRIMER_LEFT_40_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_40_SEQUENCE=AAGCTCTACTGCTGCCAAGA
PRIMER_LEFT_40=717,20
PRIMER_RIGHT_40=1665,20
PRIMER_LEFT_40_TM=59.518
PRIMER_RIGHT_40_TM=58.008
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=949
PRIMER_PAIR_41_PENALTY=2.505633
PRIMER_LEFT_41_PENALTY=1.487097
PRIMER_RIGHT_41_PENALTY=1.018537
PRIMER_LEFT_41_SEQUENCE=TAAAAGACCCGACATCGTCAA
PRIMER_RIGHT_41_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_41=399,21
PRIMER_RIGHT_41=1357,20
PRIMER_LEFT_41_TM=60.487
PRIMER_RIGHT_41_TM=61.019
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=9.1000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=959
PRIMER_PAIR_42_PENALTY=2.507618
PRIMER_LEFT_42_PENALTY=2.398418
PRIMER_RIGHT_42_PENALTY=0.109200
PRIMER_LEFT_42_SEQUENCE=GAAACTAAAGCCGCAAAATG
PRIMER_RIGHT_42_SEQUENCE=CTTCTTCTGGCGAGTCTGCT
PRIMER_LEFT_42=799,20
PRIMER_RIGHT_42=1795,20
PRIMER_LEFT_42_TM=57.602
PRIMER_RIGHT_42_TM=59.891
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.2000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=997
PRIMER_PAIR_43_PENALTY=2.537104
PRIMER_LEFT_43_PENALTY=1.034689
PRIMER_RIGHT_43_PENALTY=1.502415
PRIMER_LEFT_43_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_43_SEQUENCE=AATGGCAGAGTTTCGAAGGG
PRIMER_LEFT_43=714,20
PRIMER_RIGHT_43=1358,20
PRIMER_LEFT_43_TM=58.965
PRIMER_RIGHT_43_TM=61.502
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=645
PRIMER_PAIR_44_PENALTY=2.544005
PRIMER_LEFT_44_PENALTY=1.525468
PRIMER_RIGHT_44_PENALTY=1.018537
PRIMER_LEFT_44_SEQUENCE=TGAGTTTTTGCCTCTTTCCA
PRIMER_RIGHT_44_SEQUENCE=ATGGCAGAGTTTCGAAGGGT
PRIMER_LEFT_44=572,20
PRIMER_RIGHT_44=1357,20
PRIMER_LEFT_44_TM=58.475
PRIMER_RIGHT_44_TM=61.019
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=9.1000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=786
PRIMER_PAIR_45_PENALTY=2.554706
PRIMER_LEFT_45_PENALTY=2.398418
PRIMER_RIGHT_45_PENALTY=0.156287
PRIMER_LEFT_45_SEQUENCE=GAAACTAAAGCCGCAAAATG
PRIMER_RIGHT_45_SEQUENCE=GGCGAGTCTGCTACTTCAGG
PRIMER_LEFT_45=799,20
PRIMER_RIGHT_45=1787,20
PRIMER_LEFT_45_TM=57.602
PRIMER_RIGHT_45_TM=60.156
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=60.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.2000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=989
PRIMER_PAIR_46_PENALTY=2.568987
PRIMER_LEFT_46_PENALTY=0.481956
PRIMER_RIGHT_46_PENALTY=2.087031
PRIMER_LEFT_46_SEQUENCE=CCAATCAAAGCATCCAACAA
PRIMER_RIGHT_46_SEQUENCE=CACCGAAGAAGACGAAGCTATT
PRIMER_LEFT_46=717,20
PRIMER_RIGHT_46=1530,22
PRIMER_LEFT_46_TM=59.518
PRIMER_RIGHT_46_TM=59.913
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=5.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=814
PRIMER_PAIR_47_PENALTY=2.595023
PRIMER_LEFT_47_PENALTY=1.525468
PRIMER_RIGHT_47_PENALTY=1.069554
PRIMER_LEFT_47_SEQUENCE=TGAGTTTTTGCCTCTTTCCA
PRIMER_RIGHT_47_SEQUENCE=GGCTTCCTTGATTTCGCTAC
PRIMER_LEFT_47=572,20
PRIMER_RIGHT_47=1499,20
PRIMER_LEFT_47_TM=58.475
PRIMER_RIGHT_47_TM=58.930
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.5000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=928
PRIMER_PAIR_48_PENALTY=2.619497
PRIMER_LEFT_48_PENALTY=1.034689
PRIMER_RIGHT_48_PENALTY=1.584808
PRIMER_LEFT_48_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_48_SEQUENCE=AAAGCTCTACTGCTGCCAAGA
PRIMER_LEFT_48=714,20
PRIMER_RIGHT_48=1666,21
PRIMER_LEFT_48_TM=58.965
PRIMER_RIGHT_48_TM=59.415
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.5000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=953
PRIMER_PAIR_49_PENALTY=2.619497
PRIMER_LEFT_49_PENALTY=1.034689
PRIMER_RIGHT_49_PENALTY=1.584808
PRIMER_LEFT_49_SEQUENCE=TACCCAATCAAAGCATCCAA
PRIMER_RIGHT_49_SEQUENCE=GAAAGCTCTACTGCTGCCAAG
PRIMER_LEFT_49=714,20
PRIMER_RIGHT_49=1667,21
PRIMER_LEFT_49_TM=58.965
PRIMER_RIGHT_49_TM=59.415
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=8.5000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=954
=
SEQUENCE_ID=MAGIC.338_5_5855819_5855915_wu-0_wil-2_187243_136798_TRUE
SEQUENCE_TARGET=950,196
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTACTTTGGTGCATGATTTGAACATTTTCTTNGNAGGAGGAATCTTCATNTTTANTTTCTTNGANTTCGAGAGANANAATAGAAGATNAAAGTNTCNGAGAAAAATGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGAAGAAAGGATCATNATCTTCANNACAAATAACAAGGATGATATNGATGATNCTCTCCTTAGACCTNNNNNTATGGATCTCNATATATATTCACATGGGANNNNNNNNNNNNNNNNNNNNNNNNNNAATGTTGGCGTNTACCTACTTNGGCCTCTNTCATGACAATNATGCCCCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAANAATTTAATATCAAATAAAATTGATGAAGTTGANAAANACTNNTGACTAATNTACTTGAANCAAGTTTTAGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNAAGAAANTTATGNCATANTAACTTTGTCTTGTCGCCANTTAATAAAATTAGATTATTCAAAANNTTCATGTGNAAAACAAATCTTCATCAGGTCTCCTCAATGTCGAGTGATTNTCTTCCTTTACNTCTTCAATTTCCTTCATCATCTTCCTNNATTTCGTCNCCCANACCTCTTCTNATTCCTCTTGCTCTATNGAACCCTNGCNGAACCATCNCCAACCTCGATCTCGGGAGCCATTCGGGCATCACATTAGCATATTCGCTAGCAACTAGTTCCTCCTTGTCCTCAACTTGAGCNGGAGTCAACACTGCTAANTCAGCGTCNGCTATTGCCTCCTCTCCCTCTTTCAGTTTCTTCATCTTAGATTCATCATCACACTTCTNTGNCTCTAACCTCTTCCTCTTGAGAACCTTAACCAAACCCTCAAGCGCCGCATCANCATCTTCATCCTTCATTAGTTCCTCAGCGACTTGAGCCGGAGTCAACACGTNTCCATCAATCAAATGCNTTATATCCGGANAGAGAGGGTGAGTATCATCATTTTCGNCAGACAGGCCTAAATAGTTNGACGCCAATGTNTTAAATCCCTNAAANCTACAATGTCCCATGTAANTGTGCATATCCATACGGCCTGGTCTGAGCAATGCCGGATCCAGCTTCTCCTTGTTGTTAGTCGTAAATATTATAATACGTTCGTTTCCACAACTTGACCATAACCCATCAATGCAATTTAAAAGTCCTGATAACGTCAATGGTTTTGATACCTATTCACATNAAAAAATGTTTAAGATCGGCTCATNTATTGTTTGTACGAAAAATAATGATATAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_4_9187737_9187852_ws-0_ler-0_239541_337182_TRUE
SEQUENCE_TARGET=950,215
SEQUENCE_TEMPLATE=GTATGGACCGTTCCAAATATGTNTTTTGGGATCCTTACCATCCTACCGAAGCTGCTAANATCATCATTGCTCGGCGTCTACTCTNNGGCGACACCTCCGATATTTATCCGATNAATNTNCGGCAACTCGCTAACCTCAAGATAAATGCATGAACCGNNTAGGGAAAANNNCCCATCNATATAGGGTTGNGCGGGGNGGCTATTGACGTTTACATTTCNATCTAATTAAATATGNNNTTTNTTTTTTNAATTGATACATTGATATGCTTTTGATATTTGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNGACTTTAGTCTAATAANCTAAATATCTCANTAATCTAATANANTAGTATCTAGTTTTNATATGTCATTTTTATTTCATCANNNTTGAATGTAAGTNTGTNACNAGTATTTGTTTTNNNNNNNNNNNNNNNNNNNNNACGACTCTGTTGGAATAAANAACTCGTACCGACTCCCTATTGCAACGGTTGTTGATGGTGGTGANGNTAAATCTTNTAATCTTTAGTTCCTNAGGTANNNNATTTAATTTGGGCCATTNTANTCTTGTAATNTTNAGNCCCTTTTACTCGTTAAACACTATGAGGATTGTAANNNNNNTGGAGTTTAGACAAAANAATAANAAAAGNACACGAATGGANNCTCTCGAANNNTNTAATTTTGTTGGAGTTTTGACAAAACAAANCNNTTATCTTCGCGATTGCTTTTGTCATTTGTTNGNNNNNTTTCNTTAGTTAACAGGGNCCGCNCAATTAATTTAATTTCNNTACACTTGTACCANCTNCTTGATTCTGNTTAGGACATGATAGGTATTATTTCTGAAATTTTNCACTCGAGTGTGTGTTGAATTCGAACTTCATTTCCACAAGTTTCTCTACAATAGACTCANNTCNGNNCCCATNGGTTGTATNCCGTTCGAGAGAGAGTCTGATCCAANNGCTGGATATGNATGNTCTGTCGAACCTAATGAAGTGGCTCAAATGTATAATCTCAAGCTGAAAANACTCGTNGANGAGTTAAACAATNATCTACAAGGTTCAAGATTCGTNTACGGTGACGTTTTTCGCATTGTTTATGATATTATACAAAATTATTCATNTTACGGTACGTAANCTCAACCATTTNNNNTTCTGATAATGTTTTGAAACTTCTNTCAGATTTNAACGTATGAATTTTGTGANTTGATATTGTAGGTTTTGAGAGTGAGAAGATTCCTTGTTGTTCGTTAGTTGGGAAAGTAGGTGGGCTGATTCCATGCGGCCCGCCTTCAAAAGTATGTATGGACCGTTCCAAATATGTNTTTTGGGATCCTTACCATCCTANCGAANCTGCTAANATCATCATTGCTAGGCGTCTACTCTNCGGCGACACCTCCGATATTTATCCGATCAATNTCCGGCAACTCGCNAACCTCAAGATATATGNATGAANCGTANAANCCCCCNCCCCNCNNCTATNNNNNNTTGAGCGGGTGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.001614
PRIMER_LEFT_0_PENALTY=1.674081
PRIMER_RIGHT_0_PENALTY=0.327533
PRIMER_LEFT_0_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_0_SEQUENCE=ATCAGCCCACCTACTTTCCC
PRIMER_LEFT_0=837,20
PRIMER_RIGHT_0=1622,20
PRIMER_LEFT_0_TM=61.674
PRIMER_RIGHT_0_TM=60.328
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=786
PRIMER_PAIR_1_PENALTY=2.123910
PRIMER_LEFT_1_PENALTY=1.674081
PRIMER_RIGHT_1_PENALTY=0.449829
PRIMER_LEFT_1_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_1_SEQUENCE=TGGAATCAGCCCACCTACTT
PRIMER_LEFT_1=837,20
PRIMER_RIGHT_1=1626,20
PRIMER_LEFT_1_TM=61.674
PRIMER_RIGHT_1_TM=59.550
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=790
PRIMER_PAIR_2_PENALTY=2.600989
PRIMER_LEFT_2_PENALTY=2.273456
PRIMER_RIGHT_2_PENALTY=0.327533
PRIMER_LEFT_2_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_2_SEQUENCE=ATCAGCCCACCTACTTTCCC
PRIMER_LEFT_2=838,20
PRIMER_RIGHT_2=1622,20
PRIMER_LEFT_2_TM=62.273
PRIMER_RIGHT_2_TM=60.328
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=785
PRIMER_PAIR_3_PENALTY=2.634850
PRIMER_LEFT_3_PENALTY=2.273456
PRIMER_RIGHT_3_PENALTY=0.361394
PRIMER_LEFT_3_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_3_SEQUENCE=GCCCACCTACTTTCCCAACT
PRIMER_LEFT_3=838,20
PRIMER_RIGHT_3=1618,20
PRIMER_LEFT_3_TM=62.273
PRIMER_RIGHT_3_TM=60.361
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=781
PRIMER_PAIR_4_PENALTY=2.719912
PRIMER_LEFT_4_PENALTY=1.674081
PRIMER_RIGHT_4_PENALTY=1.045831
PRIMER_LEFT_4_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_4_SEQUENCE=GAATCAGCCCACCTACTTTCC
PRIMER_LEFT_4=837,20
PRIMER_RIGHT_4=1624,21
PRIMER_LEFT_4_TM=61.674
PRIMER_RIGHT_4_TM=59.954
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=788
PRIMER_PAIR_5_PENALTY=2.723285
PRIMER_LEFT_5_PENALTY=2.273456
PRIMER_RIGHT_5_PENALTY=0.449829
PRIMER_LEFT_5_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_5_SEQUENCE=TGGAATCAGCCCACCTACTT
PRIMER_LEFT_5=838,20
PRIMER_RIGHT_5=1626,20
PRIMER_LEFT_5_TM=62.273
PRIMER_RIGHT_5_TM=59.550
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=5.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=789
PRIMER_PAIR_6_PENALTY=2.833444
PRIMER_LEFT_6_PENALTY=1.674081
PRIMER_RIGHT_6_PENALTY=1.159363
PRIMER_LEFT_6_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_6_SEQUENCE=ATGGAATCAGCCCACCTACTT
PRIMER_LEFT_6=837,20
PRIMER_RIGHT_6=1627,21
PRIMER_LEFT_6_TM=61.674
PRIMER_RIGHT_6_TM=59.841
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=791
PRIMER_PAIR_7_PENALTY=2.984501
PRIMER_LEFT_7_PENALTY=1.674081
PRIMER_RIGHT_7_PENALTY=1.310420
PRIMER_LEFT_7_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_7_SEQUENCE=GGATGGTAAGGATCCCAAAA
PRIMER_LEFT_7=837,20
PRIMER_RIGHT_7=1691,20
PRIMER_LEFT_7_TM=61.674
PRIMER_RIGHT_7_TM=58.690
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=855
PRIMER_PAIR_8_PENALTY=3.021725
PRIMER_LEFT_8_PENALTY=1.674081
PRIMER_RIGHT_8_PENALTY=1.347645
PRIMER_LEFT_8_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_8_SEQUENCE=AGGATGGTAAGGATCCCAAAA
PRIMER_LEFT_8=837,20
PRIMER_RIGHT_8=1692,21
PRIMER_LEFT_8_TM=61.674
PRIMER_RIGHT_8_TM=59.652
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=856
PRIMER_PAIR_9_PENALTY=3.153412
PRIMER_LEFT_9_PENALTY=1.674081
PRIMER_RIGHT_9_PENALTY=1.479331
PRIMER_LEFT_9_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_9_SEQUENCE=CCCAACTAACGAACAACAAGG
PRIMER_LEFT_9=837,20
PRIMER_RIGHT_9=1605,21
PRIMER_LEFT_9_TM=61.674
PRIMER_RIGHT_9_TM=59.521
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=769
PRIMER_PAIR_10_PENALTY=3.200654
PRIMER_LEFT_10_PENALTY=1.674081
PRIMER_RIGHT_10_PENALTY=1.526573
PRIMER_LEFT_10_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_10_SEQUENCE=ATGGAATCAGCCCACCTACT
PRIMER_LEFT_10=837,20
PRIMER_RIGHT_10=1627,20
PRIMER_LEFT_10_TM=61.674
PRIMER_RIGHT_10_TM=58.473
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=5.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=791
PRIMER_PAIR_11_PENALTY=3.319287
PRIMER_LEFT_11_PENALTY=2.273456
PRIMER_RIGHT_11_PENALTY=1.045831
PRIMER_LEFT_11_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_11_SEQUENCE=GAATCAGCCCACCTACTTTCC
PRIMER_LEFT_11=838,20
PRIMER_RIGHT_11=1624,21
PRIMER_LEFT_11_TM=62.273
PRIMER_RIGHT_11_TM=59.954
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=787
PRIMER_PAIR_12_PENALTY=3.319287
PRIMER_LEFT_12_PENALTY=2.273456
PRIMER_RIGHT_12_PENALTY=1.045831
PRIMER_LEFT_12_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_12_SEQUENCE=GGAATCAGCCCACCTACTTTC
PRIMER_LEFT_12=838,20
PRIMER_RIGHT_12=1625,21
PRIMER_LEFT_12_TM=62.273
PRIMER_RIGHT_12_TM=59.954
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=788
PRIMER_PAIR_13_PENALTY=3.428576
PRIMER_LEFT_13_PENALTY=1.674081
PRIMER_RIGHT_13_PENALTY=1.754495
PRIMER_LEFT_13_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_13_SEQUENCE=TCAGACTCTCTCTCGAACGG
PRIMER_LEFT_13=837,20
PRIMER_RIGHT_13=1302,20
PRIMER_LEFT_13_TM=61.674
PRIMER_RIGHT_13_TM=58.246
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=466
PRIMER_PAIR_14_PENALTY=3.432819
PRIMER_LEFT_14_PENALTY=2.273456
PRIMER_RIGHT_14_PENALTY=1.159363
PRIMER_LEFT_14_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_14_SEQUENCE=ATGGAATCAGCCCACCTACTT
PRIMER_LEFT_14=838,20
PRIMER_RIGHT_14=1627,21
PRIMER_LEFT_14_TM=62.273
PRIMER_RIGHT_14_TM=59.841
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=790
PRIMER_PAIR_15_PENALTY=3.534436
PRIMER_LEFT_15_PENALTY=1.674081
PRIMER_RIGHT_15_PENALTY=1.860355
PRIMER_LEFT_15_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_15_SEQUENCE=TGGAATCAGCCCACCTACTTT
PRIMER_LEFT_15=837,20
PRIMER_RIGHT_15=1626,21
PRIMER_LEFT_15_TM=61.674
PRIMER_RIGHT_15_TM=60.860
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PAIR_15_PRODUCT_SIZE=790
PRIMER_PAIR_16_PENALTY=3.553228
PRIMER_LEFT_16_PENALTY=1.674081
PRIMER_RIGHT_16_PENALTY=1.879147
PRIMER_LEFT_16_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_16_SEQUENCE=AATCAGCCCACCTACTTTCC
PRIMER_LEFT_16=837,20
PRIMER_RIGHT_16=1623,20
PRIMER_LEFT_16_TM=61.674
PRIMER_RIGHT_16_TM=58.121
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=787
PRIMER_PAIR_17_PENALTY=3.553228
PRIMER_LEFT_17_PENALTY=1.674081
PRIMER_RIGHT_17_PENALTY=1.879147
PRIMER_LEFT_17_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_17_SEQUENCE=GGAATCAGCCCACCTACTTT
PRIMER_LEFT_17=837,20
PRIMER_RIGHT_17=1625,20
PRIMER_LEFT_17_TM=61.674
PRIMER_RIGHT_17_TM=58.121
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=789
PRIMER_PAIR_18_PENALTY=3.583876
PRIMER_LEFT_18_PENALTY=2.273456
PRIMER_RIGHT_18_PENALTY=1.310420
PRIMER_LEFT_18_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_18_SEQUENCE=GGATGGTAAGGATCCCAAAA
PRIMER_LEFT_18=838,20
PRIMER_RIGHT_18=1691,20
PRIMER_LEFT_18_TM=62.273
PRIMER_RIGHT_18_TM=58.690
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=854
PRIMER_PAIR_19_PENALTY=3.621101
PRIMER_LEFT_19_PENALTY=2.273456
PRIMER_RIGHT_19_PENALTY=1.347645
PRIMER_LEFT_19_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_19_SEQUENCE=AGGATGGTAAGGATCCCAAAA
PRIMER_LEFT_19=838,20
PRIMER_RIGHT_19=1692,21
PRIMER_LEFT_19_TM=62.273
PRIMER_RIGHT_19_TM=59.652
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=855
PRIMER_PAIR_20_PENALTY=3.752788
PRIMER_LEFT_20_PENALTY=2.273456
PRIMER_RIGHT_20_PENALTY=1.479331
PRIMER_LEFT_20_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_20_SEQUENCE=CCCAACTAACGAACAACAAGG
PRIMER_LEFT_20=838,20
PRIMER_RIGHT_20=1605,21
PRIMER_LEFT_20_TM=62.273
PRIMER_RIGHT_20_TM=59.521
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=8.5000
PRIMER_PAIR_20_COMPL_ANY=7.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=768
PRIMER_PAIR_21_PENALTY=3.891484
PRIMER_LEFT_21_PENALTY=1.674081
PRIMER_RIGHT_21_PENALTY=2.217403
PRIMER_LEFT_21_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_21_SEQUENCE=TACATACTTTTGAAGGCGGG
PRIMER_LEFT_21=837,20
PRIMER_RIGHT_21=1651,20
PRIMER_LEFT_21_TM=61.674
PRIMER_RIGHT_21_TM=57.783
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=12.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=815
PRIMER_PAIR_22_PENALTY=3.945823
PRIMER_LEFT_22_PENALTY=1.674081
PRIMER_RIGHT_22_PENALTY=2.271742
PRIMER_LEFT_22_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_22_SEQUENCE=CATGGAATCAGCCCACCTACT
PRIMER_LEFT_22=837,20
PRIMER_RIGHT_22=1628,21
PRIMER_LEFT_22_TM=61.674
PRIMER_RIGHT_22_TM=61.272
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=5.4000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=792
PRIMER_PAIR_23_PENALTY=3.999666
PRIMER_LEFT_23_PENALTY=1.674081
PRIMER_RIGHT_23_PENALTY=2.325585
PRIMER_LEFT_23_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_23_SEQUENCE=TAGGATGGTAAGGATCCCAAAA
PRIMER_LEFT_23=837,20
PRIMER_RIGHT_23=1693,22
PRIMER_LEFT_23_TM=61.674
PRIMER_RIGHT_23_TM=59.674
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=40.909
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=857
PRIMER_PAIR_24_PENALTY=4.027951
PRIMER_LEFT_24_PENALTY=2.273456
PRIMER_RIGHT_24_PENALTY=1.754495
PRIMER_LEFT_24_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_24_SEQUENCE=TCAGACTCTCTCTCGAACGG
PRIMER_LEFT_24=838,20
PRIMER_RIGHT_24=1302,20
PRIMER_LEFT_24_TM=62.273
PRIMER_RIGHT_24_TM=58.246
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=9.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=465
PRIMER_PAIR_25_PENALTY=4.042160
PRIMER_LEFT_25_PENALTY=1.674081
PRIMER_RIGHT_25_PENALTY=2.368079
PRIMER_LEFT_25_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_25_SEQUENCE=ATCAGACTCTCTCTCGAACGG
PRIMER_LEFT_25=837,20
PRIMER_RIGHT_25=1303,21
PRIMER_LEFT_25_TM=61.674
PRIMER_RIGHT_25_TM=58.632
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=52.381
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=467
PRIMER_PAIR_26_PENALTY=4.133811
PRIMER_LEFT_26_PENALTY=2.273456
PRIMER_RIGHT_26_PENALTY=1.860355
PRIMER_LEFT_26_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_26_SEQUENCE=TGGAATCAGCCCACCTACTTT
PRIMER_LEFT_26=838,20
PRIMER_RIGHT_26=1626,21
PRIMER_LEFT_26_TM=62.273
PRIMER_RIGHT_26_TM=60.860
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=789
PRIMER_PAIR_27_PENALTY=4.152603
PRIMER_LEFT_27_PENALTY=2.273456
PRIMER_RIGHT_27_PENALTY=1.879147
PRIMER_LEFT_27_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_27_SEQUENCE=AATCAGCCCACCTACTTTCC
PRIMER_LEFT_27=838,20
PRIMER_RIGHT_27=1623,20
PRIMER_LEFT_27_TM=62.273
PRIMER_RIGHT_27_TM=58.121
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.9000
PRIMER_RIGHT_27_END_STABILITY=8.5000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=786
PRIMER_PAIR_28_PENALTY=4.152603
PRIMER_LEFT_28_PENALTY=2.273456
PRIMER_RIGHT_28_PENALTY=1.879147
PRIMER_LEFT_28_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_28_SEQUENCE=GGAATCAGCCCACCTACTTT
PRIMER_LEFT_28=838,20
PRIMER_RIGHT_28=1625,20
PRIMER_LEFT_28_TM=62.273
PRIMER_RIGHT_28_TM=58.121
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=788
PRIMER_PAIR_29_PENALTY=4.205824
PRIMER_LEFT_29_PENALTY=1.674081
PRIMER_RIGHT_29_PENALTY=2.531743
PRIMER_LEFT_29_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_29_SEQUENCE=GATCAGACTCTCTCTCGAACGG
PRIMER_LEFT_29=837,20
PRIMER_RIGHT_29=1304,22
PRIMER_LEFT_29_TM=61.674
PRIMER_RIGHT_29_TM=60.532
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=54.545
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=9.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=468
PRIMER_PAIR_30_PENALTY=4.233790
PRIMER_LEFT_30_PENALTY=1.674081
PRIMER_RIGHT_30_PENALTY=2.559709
PRIMER_LEFT_30_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_30_SEQUENCE=AATCAGCCCACCTACTTTCCC
PRIMER_LEFT_30=837,20
PRIMER_RIGHT_30=1623,21
PRIMER_LEFT_30_TM=61.674
PRIMER_RIGHT_30_TM=61.560
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=787
PRIMER_PAIR_31_PENALTY=4.268290
PRIMER_LEFT_31_PENALTY=1.674081
PRIMER_RIGHT_31_PENALTY=2.594209
PRIMER_LEFT_31_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_31_SEQUENCE=CGGATAAATATCGGAGGTGTC
PRIMER_LEFT_31=837,20
PRIMER_RIGHT_31=1758,21
PRIMER_LEFT_31_TM=61.674
PRIMER_RIGHT_31_TM=58.406
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=6.1000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=922
PRIMER_PAIR_32_PENALTY=4.319299
PRIMER_LEFT_32_PENALTY=1.674081
PRIMER_RIGHT_32_PENALTY=2.645218
PRIMER_LEFT_32_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_32_SEQUENCE=CGAACAACAAGGAATCTTCTCA
PRIMER_LEFT_32=837,20
PRIMER_RIGHT_32=1596,22
PRIMER_LEFT_32_TM=61.674
PRIMER_RIGHT_32_TM=59.355
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.909
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=6.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=760
PRIMER_PAIR_33_PENALTY=4.385118
PRIMER_LEFT_33_PENALTY=1.674081
PRIMER_RIGHT_33_PENALTY=2.711037
PRIMER_LEFT_33_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_33_SEQUENCE=CATACATACTTTTGAAGGCGGG
PRIMER_LEFT_33=837,20
PRIMER_RIGHT_33=1653,22
PRIMER_LEFT_33_TM=61.674
PRIMER_RIGHT_33_TM=60.711
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=12.9000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=817
PRIMER_PAIR_34_PENALTY=4.385118
PRIMER_LEFT_34_PENALTY=1.674081
PRIMER_RIGHT_34_PENALTY=2.711037
PRIMER_LEFT_34_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_34_SEQUENCE=CCATACATACTTTTGAAGGCGG
PRIMER_LEFT_34=837,20
PRIMER_RIGHT_34=1654,22
PRIMER_LEFT_34_TM=61.674
PRIMER_RIGHT_34_TM=60.711
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.455
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=12.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=818
PRIMER_PAIR_35_PENALTY=4.435721
PRIMER_LEFT_35_PENALTY=1.674081
PRIMER_RIGHT_35_PENALTY=2.761640
PRIMER_LEFT_35_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_35_SEQUENCE=CCACTTCATTAGGTTCGACAGA
PRIMER_LEFT_35=837,20
PRIMER_RIGHT_35=1346,22
PRIMER_LEFT_35_TM=61.674
PRIMER_RIGHT_35_TM=59.238
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.455
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=510
PRIMER_PAIR_36_PENALTY=4.456661
PRIMER_LEFT_36_PENALTY=4.129128
PRIMER_RIGHT_36_PENALTY=0.327533
PRIMER_LEFT_36_SEQUENCE=AACTCGTACCGACTCCCTATT
PRIMER_RIGHT_36_SEQUENCE=ATCAGCCCACCTACTTTCCC
PRIMER_LEFT_36=814,21
PRIMER_RIGHT_36=1622,20
PRIMER_LEFT_36_TM=56.871
PRIMER_RIGHT_36_TM=60.328
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.9000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=809
PRIMER_PAIR_37_PENALTY=4.468748
PRIMER_LEFT_37_PENALTY=4.129128
PRIMER_RIGHT_37_PENALTY=0.339620
PRIMER_LEFT_37_SEQUENCE=AACTCGTACCGACTCCCTATT
PRIMER_RIGHT_37_SEQUENCE=CATGGAATCAGCCCACCTAC
PRIMER_LEFT_37=814,21
PRIMER_RIGHT_37=1628,20
PRIMER_LEFT_37_TM=56.871
PRIMER_RIGHT_37_TM=60.340
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=5.9000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=815
PRIMER_PAIR_38_PENALTY=4.490522
PRIMER_LEFT_38_PENALTY=4.129128
PRIMER_RIGHT_38_PENALTY=0.361394
PRIMER_LEFT_38_SEQUENCE=AACTCGTACCGACTCCCTATT
PRIMER_RIGHT_38_SEQUENCE=GCCCACCTACTTTCCCAACT
PRIMER_LEFT_38=814,21
PRIMER_RIGHT_38=1618,20
PRIMER_LEFT_38_TM=56.871
PRIMER_RIGHT_38_TM=60.361
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=5.9000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=805
PRIMER_PAIR_39_PENALTY=4.490522
PRIMER_LEFT_39_PENALTY=4.129128
PRIMER_RIGHT_39_PENALTY=0.361394
PRIMER_LEFT_39_SEQUENCE=AACTCGTACCGACTCCCTATT
PRIMER_RIGHT_39_SEQUENCE=AGCCCACCTACTTTCCCAAC
PRIMER_LEFT_39=814,21
PRIMER_RIGHT_39=1619,20
PRIMER_LEFT_39_TM=56.871
PRIMER_RIGHT_39_TM=60.361
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=5.9000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=806
PRIMER_PAIR_40_PENALTY=4.490859
PRIMER_LEFT_40_PENALTY=2.273456
PRIMER_RIGHT_40_PENALTY=2.217403
PRIMER_LEFT_40_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_40_SEQUENCE=TACATACTTTTGAAGGCGGG
PRIMER_LEFT_40=838,20
PRIMER_RIGHT_40=1651,20
PRIMER_LEFT_40_TM=62.273
PRIMER_RIGHT_40_TM=57.783
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=3.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=12.9000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=814
PRIMER_PAIR_41_PENALTY=4.500497
PRIMER_LEFT_41_PENALTY=1.674081
PRIMER_RIGHT_41_PENALTY=2.826416
PRIMER_LEFT_41_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_41_SEQUENCE=CCAACTAACGAACAACAAGGAA
PRIMER_LEFT_41=837,20
PRIMER_RIGHT_41=1604,22
PRIMER_LEFT_41_TM=61.674
PRIMER_RIGHT_41_TM=59.174
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.909
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=768
PRIMER_PAIR_42_PENALTY=4.515233
PRIMER_LEFT_42_PENALTY=2.273456
PRIMER_RIGHT_42_PENALTY=2.241777
PRIMER_LEFT_42_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_42_SEQUENCE=AGCCCACCTACTTTCCCAACT
PRIMER_LEFT_42=838,20
PRIMER_RIGHT_42=1619,21
PRIMER_LEFT_42_TM=62.273
PRIMER_RIGHT_42_TM=61.242
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=782
PRIMER_PAIR_43_PENALTY=4.523533
PRIMER_LEFT_43_PENALTY=1.674081
PRIMER_RIGHT_43_PENALTY=2.849452
PRIMER_LEFT_43_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_43_SEQUENCE=ATACATACTTTTGAAGGCGGG
PRIMER_LEFT_43=837,20
PRIMER_RIGHT_43=1652,21
PRIMER_LEFT_43_TM=61.674
PRIMER_RIGHT_43_TM=58.151
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=12.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=816
PRIMER_PAIR_44_PENALTY=4.548072
PRIMER_LEFT_44_PENALTY=1.674081
PRIMER_RIGHT_44_PENALTY=2.873991
PRIMER_LEFT_44_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_44_SEQUENCE=TCAGCCCACCTACTTTCCCA
PRIMER_LEFT_44=837,20
PRIMER_RIGHT_44=1621,20
PRIMER_LEFT_44_TM=61.674
PRIMER_RIGHT_44_TM=62.874
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=9.7000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=785
PRIMER_PAIR_45_PENALTY=4.562652
PRIMER_LEFT_45_PENALTY=1.674081
PRIMER_RIGHT_45_PENALTY=2.888571
PRIMER_LEFT_45_SEQUENCE=AACGGTTGTTGATGGTGGTG
PRIMER_RIGHT_45_SEQUENCE=CCCACCTACTTTCCCAACTAA
PRIMER_LEFT_45=837,20
PRIMER_RIGHT_45=1617,21
PRIMER_LEFT_45_TM=61.674
PRIMER_RIGHT_45_TM=58.111
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=5.7000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=781
PRIMER_PAIR_46_PENALTY=4.574054
PRIMER_LEFT_46_PENALTY=2.273456
PRIMER_RIGHT_46_PENALTY=2.300598
PRIMER_LEFT_46_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_46_SEQUENCE=CAGCCCACCTACTTTCCCAA
PRIMER_LEFT_46=838,20
PRIMER_RIGHT_46=1620,20
PRIMER_LEFT_46_TM=62.273
PRIMER_RIGHT_46_TM=62.301
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=10.0000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=783
PRIMER_PAIR_47_PENALTY=4.578957
PRIMER_LEFT_47_PENALTY=4.129128
PRIMER_RIGHT_47_PENALTY=0.449829
PRIMER_LEFT_47_SEQUENCE=AACTCGTACCGACTCCCTATT
PRIMER_RIGHT_47_SEQUENCE=TGGAATCAGCCCACCTACTT
PRIMER_LEFT_47=814,21
PRIMER_RIGHT_47=1626,20
PRIMER_LEFT_47_TM=56.871
PRIMER_RIGHT_47_TM=59.550
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=5.9000
PRIMER_RIGHT_47_END_STABILITY=5.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=813
PRIMER_PAIR_48_PENALTY=4.599041
PRIMER_LEFT_48_PENALTY=2.273456
PRIMER_RIGHT_48_PENALTY=2.325585
PRIMER_LEFT_48_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_48_SEQUENCE=TAGGATGGTAAGGATCCCAAAA
PRIMER_LEFT_48=838,20
PRIMER_RIGHT_48=1693,22
PRIMER_LEFT_48_TM=62.273
PRIMER_RIGHT_48_TM=59.674
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=856
PRIMER_PAIR_49_PENALTY=4.641535
PRIMER_LEFT_49_PENALTY=2.273456
PRIMER_RIGHT_49_PENALTY=2.368079
PRIMER_LEFT_49_SEQUENCE=ACGGTTGTTGATGGTGGTGA
PRIMER_RIGHT_49_SEQUENCE=ATCAGACTCTCTCTCGAACGG
PRIMER_LEFT_49=838,20
PRIMER_RIGHT_49=1303,21
PRIMER_LEFT_49_TM=62.273
PRIMER_RIGHT_49_TM=58.632
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=9.9000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=466
=
SEQUENCE_ID=MAGIC.433_1_16021587_16021946_ws-0_ct-1_15374_10538_TRUE
SEQUENCE_TARGET=950,459
SEQUENCE_TEMPLATE=ATATANTNANAANAAATATAAACCTAAGCGGNAAATTTNCCATCTATCCNAAATTAGGTTAANAATANGATATTGGTTTAATGNNNNAANACTANATANNNNTTATATAAANCGTNACATAAANTTAGANNNNNNNNNNNNNNNNNTCTTTTAATGGGAGACTCACAAGAGAGCATAANAATGTGTGGTNNNNNNNNNNGTTGTNGAGAAAGCAAGGGTTCAAATGTTGTTATGGAGGTATTNTAAACCGTTTANATGAATCNAGTCATCTCTTTGATTTTNTAANTATNAACTTACTTTTAANTTTGAAGGTGATTTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGNGTTTGAGGATGAANTNAGAATGNTGGNGAATTGTTNTTGANTNTTGTTTTTNGGNAATTGTTCTNGAANNTTTNTTTTGGATTGCTTCTAATGTAAGTCGGGTGAGAANTTTAAGNTTCTTTNATGGTATCTTATTCTCATTTNNGTATCAGTTTGCTATNNTGTTTATGTTATTAACTTTTNGAATCNATGGGANTTTATCCATATGANATNGNATTTGTAATGCANCATTTATAAATATTTTTAGAATTATGTTAANAAATTTGGCTAANAANTTTNTTGNANGNTTGAAATTAGCATATTANCCTAAATNANAGGTTTAACAGTATGGTAATTAGNTANNANGTTTGAACCNATTNGATTCCNTTAGGNAATTAGCNAAAANNTTTGAACNGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGTTCTTCANATATTNATTTGACTAAANTGGATANGTNTATGTGGATAACGATAATTANANNCAAGTAATAATANGTTAANAANCAATAAATNTTAATTTAAACCAAAANATGTATATGNNNNNNNNNNNNNNTGGATACNTCTATTTGTATACNTNTTTANNTAAAAGGTTTGAACCGNTTANATTNNCTTANGTAATTTTNAAAAGGTTTNGACCNATTANATNTTCTTTANATATTTATTTGACTAAANTGNATANGTCTATTTGTATAACGATAATTATAGGCAAGTAATAANATGTTAAGNAACNANNAATAANNNTTTNAAACTAAANANNTATATNTGGATACGTCTNTGNGGATACGTNAATTTGGATATAGATNATAAACTAAATTTGTATATNTGGATACGTCNATTANTATTAGTCCACGAACTTTTTAGATTTCTAAATATTTTNGATATCACNACNATNCNTAATATACAATATAAATCAGAATNTATAAATCATATAGAAAGAANATGCGATAAGGATATNNAAACCACCGCCNGACCACATGGAGTTAAANCGAATTGTTNGANTTTTTNNNNNNNNAANGTCATGATGAATTTTGNAGGAAAAGNTAATTAGATCTATATATAGGACCTTCATAGCCTATAGCGTTAGGGGCGTGTGTAANTTGTTATTGANAATCGTTCNATGCAGNCGCTTACACCACCTAATGACATATCAATCAATCAACTNTTTAAACGGCTNTTTAATTTNTTTNCCACAAAACACATTANCNAATGTAAGCTCAATTATTATTTGACATATGNTGTTANACCTTTACANNANAAANTCNAAAAGTTGTACTTTTATTCCANTAAATATCAATTTTAAACAGATGATATAGGTATCCATGTGGTTGTNATTATGTAATTATATTGCATAATAGTCTTATAAACTTAGGTAAACTTACTTAAATTAGAGTAGTGCTGAGTTTTAGGAGNATAATTAGTTNGTACAACAAAGNACATTNNAACAAAAAACATTACAATAGGCGACCACCTGCATATTATGTGAAANAATATAGANAAGTCATTAANNAGAGTNCATATAACNTAGAATGATACCATTATAGAAACACCTAGAACTAGAAN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_17415060_17415082_ws-0_can-0_7323_22772_TRUE
SEQUENCE_TARGET=950,122
SEQUENCE_TEMPLATE=TGGCGCGTGACAGACACTCTCCNTCTCTCCATGNCCATAAAATCTCAAAGACTGTTTNAAAAAAAAAATGTTTTAGCTTTAACTGCTTTTTTNTTGTTGTTGGTGTAATGATATCACAGAGAGAAGAAAGAGAAGAGAAGAAGCAGAGAGTGATGGGAGATAAGAAATTGATTTCATCTTCTTCTTCTTCCTCGGTTTACGATACTCGTATCAATCATCATCTTCATCATCCTCCGTCTTCTTCCGACGAAATCTCTCAGTTTCTCCGNCATATTTTCGACCGTTCTTCTCCTTTACCTTCTTACTACTCCCCGGCGACGACTACAACGACGGNGTCTTTGATTGGTGTGCACGGGAGCGGTGACCCACATGCAGATAACTCGAGAAGTCTCGTTTCTCATCATCCACCGTCAGATTCNGTGCTTATGTCGAAACGTGTCGGAGATTTCTCTGAGGTTTTAATCGGCGGAGGATCAGGCTCAGCCGCCGCGTGTTTTGNTTTCTCCGGTGGTGGTAATAATAACAACGTTCAAGGAAATAGCTCTGGGACTCGAGTATCGTCTTCTTCCGTTGGAGCTAGTGGCAACGAGACAGATGAGTATGACTGTGAAAGCGAGGTTTTTTCTCAATCTCTNTTTTTTATCTTTTGCATGTTTTAAAAGTTGANTCTTTGTCTCTTTCCCGAGAAAATNTTTCTACAGAGATTTCTCTTTAACGGATCTGTACTTTATTTNCCCCCTTAAAATCCGCANTTTTTTTGGTTGTGATTTTGAGTGAAGTTTTGTCATTTGTGAGTGTAATAATGAATGCACACCGAGTGTTTNNTAANTTGCTTNAATGAAAANACAGGAAGGAGGAGAAGCTGTAGTTGATGAAGCTCCCTCTTCCAAGTCAGGTCCTTNTTCTCGTAGTTCATCTAAAAGATGCAGAGCTGCTGAAGTTCATAATCTCTCTGAGAAGGTTTGGTTTNGANTCTCTCCCAAAAGTAGAAACTTTCTTANTTGAGANNNNNNNNNNNNNTTNTTGTAACAGAGGAGGAGAAGTAGAATTAATGAAAAAATGAAAGCTTTACAAAGTCTCATCCCTAATTCAAATAAGGTAATGATACTATATATAGAGAGACATCTACATGAATCATTCTATTTNCTGTAGATTCTTTTTGTGTCTGATTATTGCAATGTGTTTATTGGGGAAGACGGATAAGGCTTCAATGCTTGATGAAGCCATTGAGTATCTGAAACAGCTTCAGCTCCAAGTTCAGGTCACTAAAGATACCTTATGTTTATCATTATAACCAAAATCCTAAAACCAGTCTATATTCTGACATAAGTCTTTTTTTGTTCTGATATTTGCAGATGTTGACTATGAGAAATGGAATAAACTTGCATCCTTTGTGTTTACCTGGAACTACATTACACCCATTGCAACTCTCTCAGATTCGACCCCCTGAAGCAACCAATGATCCTCTGCTTAATCATACCNATCAGTTTGCTTCGACTTCTAATGCACCGGAAATGATCAATACTGTGGCTTCTTCATACGCTTTGGAACCTTCTATTCGCAGTCACTTTGGACCTTTCCCTCTCNTTACTTCACCCGTGGTGCGTAGTTGAATTACACATTTTTCATTCTATAACCATAAAGCTAGTTTGCTTACTAATTTTTTTTTGTTACTTAAGGAGATGAGTCGGGAAGGTGGGTTAACTCATCCAAGGTTGAACATTGGTCATTCCAACGCAAACATAACCGGTAAAGTCTTGTTGTTTTGGAATCCTAAGGAAGTTTTAGGACATTTACAATGTTAATAACGTTACGATATTTGGGGATTTGCAGGGGAACAAGCTCTGTTTGATGGACAACCTGACCTAAAAGATCGAATTACTTGAACAGTGTCCCAACTTCGGGATCTCTATGTGTTCTTGTTTCTTAGAACGCAAGCCATAAAGCTGTCTGACAATGGAGATGCATTTAACTGTCTTTATTTTTTCTAAGGTTCTATTTAAAATCAATTGTTGGTGACAC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.086404
PRIMER_LEFT_0_PENALTY=0.047584
PRIMER_RIGHT_0_PENALTY=0.038821
PRIMER_LEFT_0_SEQUENCE=TTTCTCATCATCCACCGTCA
PRIMER_RIGHT_0_SEQUENCE=GAAGCCTTATCCGTCTTCCC
PRIMER_LEFT_0=393,20
PRIMER_RIGHT_0=1208,20
PRIMER_LEFT_0_TM=60.048
PRIMER_RIGHT_0_TM=60.039
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.4000
PRIMER_RIGHT_0_END_STABILITY=9.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=816
PRIMER_PAIR_1_PENALTY=0.348774
PRIMER_LEFT_1_PENALTY=0.047584
PRIMER_RIGHT_1_PENALTY=0.301190
PRIMER_LEFT_1_SEQUENCE=TTTCTCATCATCCACCGTCA
PRIMER_RIGHT_1_SEQUENCE=TCCGTCTTCCCCAATAAACA
PRIMER_LEFT_1=393,20
PRIMER_RIGHT_1=1199,20
PRIMER_LEFT_1_TM=60.048
PRIMER_RIGHT_1_TM=60.301
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=807
PRIMER_PAIR_2_PENALTY=0.373959
PRIMER_LEFT_2_PENALTY=0.047584
PRIMER_RIGHT_2_PENALTY=0.326376
PRIMER_LEFT_2_SEQUENCE=TTTCTCATCATCCACCGTCA
PRIMER_RIGHT_2_SEQUENCE=GCATTGAAGCCTTATCCGTC
PRIMER_LEFT_2=393,20
PRIMER_RIGHT_2=1213,20
PRIMER_LEFT_2_TM=60.048
PRIMER_RIGHT_2_TM=59.674
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=821
PRIMER_PAIR_3_PENALTY=0.408028
PRIMER_LEFT_3_PENALTY=0.369208
PRIMER_RIGHT_3_PENALTY=0.038821
PRIMER_LEFT_3_SEQUENCE=TTCTCATCATCCACCGTCAG
PRIMER_RIGHT_3_SEQUENCE=GAAGCCTTATCCGTCTTCCC
PRIMER_LEFT_3=394,20
PRIMER_RIGHT_3=1208,20
PRIMER_LEFT_3_TM=59.631
PRIMER_RIGHT_3_TM=60.039
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=815
PRIMER_PAIR_4_PENALTY=0.427060
PRIMER_LEFT_4_PENALTY=0.388240
PRIMER_RIGHT_4_PENALTY=0.038821
PRIMER_LEFT_4_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_4_SEQUENCE=GAAGCCTTATCCGTCTTCCC
PRIMER_LEFT_4=563,20
PRIMER_RIGHT_4=1208,20
PRIMER_LEFT_4_TM=60.388
PRIMER_RIGHT_4_TM=60.039
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=646
PRIMER_PAIR_5_PENALTY=0.543118
PRIMER_LEFT_5_PENALTY=0.388240
PRIMER_RIGHT_5_PENALTY=0.154878
PRIMER_LEFT_5_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_5_SEQUENCE=CCAAAGCGTATGAAGAAGCC
PRIMER_LEFT_5=563,20
PRIMER_RIGHT_5=1547,20
PRIMER_LEFT_5_TM=60.388
PRIMER_RIGHT_5_TM=59.845
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=985
PRIMER_PAIR_6_PENALTY=0.557711
PRIMER_LEFT_6_PENALTY=0.388240
PRIMER_RIGHT_6_PENALTY=0.169471
PRIMER_LEFT_6_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_6_SEQUENCE=TGAGAGAGTTGCAATGGGTG
PRIMER_LEFT_6=563,20
PRIMER_RIGHT_6=1434,20
PRIMER_LEFT_6_TM=60.388
PRIMER_RIGHT_6_TM=59.831
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=872
PRIMER_PAIR_7_PENALTY=0.614383
PRIMER_LEFT_7_PENALTY=0.388240
PRIMER_RIGHT_7_PENALTY=0.226143
PRIMER_LEFT_7_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_7_SEQUENCE=GCAGAGGATCATTGGTTGCT
PRIMER_LEFT_7=563,20
PRIMER_RIGHT_7=1469,20
PRIMER_LEFT_7_TM=60.388
PRIMER_RIGHT_7_TM=60.226
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=907
PRIMER_PAIR_8_PENALTY=0.618968
PRIMER_LEFT_8_PENALTY=0.580148
PRIMER_RIGHT_8_PENALTY=0.038821
PRIMER_LEFT_8_SEQUENCE=AGTGGCAACGAGACAGATGA
PRIMER_RIGHT_8_SEQUENCE=GAAGCCTTATCCGTCTTCCC
PRIMER_LEFT_8=578,20
PRIMER_RIGHT_8=1208,20
PRIMER_LEFT_8_TM=59.420
PRIMER_RIGHT_8_TM=60.039
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=631
PRIMER_PAIR_9_PENALTY=0.634643
PRIMER_LEFT_9_PENALTY=0.595822
PRIMER_RIGHT_9_PENALTY=0.038821
PRIMER_LEFT_9_SEQUENCE=ATCCTCCGTCTTCTTCCGAC
PRIMER_RIGHT_9_SEQUENCE=GAAGCCTTATCCGTCTTCCC
PRIMER_LEFT_9=228,20
PRIMER_RIGHT_9=1208,20
PRIMER_LEFT_9_TM=60.596
PRIMER_RIGHT_9_TM=60.039
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=981
PRIMER_PAIR_10_PENALTY=0.670398
PRIMER_LEFT_10_PENALTY=0.369208
PRIMER_RIGHT_10_PENALTY=0.301190
PRIMER_LEFT_10_SEQUENCE=TTCTCATCATCCACCGTCAG
PRIMER_RIGHT_10_SEQUENCE=TCCGTCTTCCCCAATAAACA
PRIMER_LEFT_10=394,20
PRIMER_RIGHT_10=1199,20
PRIMER_LEFT_10_TM=59.631
PRIMER_RIGHT_10_TM=60.301
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=2.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=806
PRIMER_PAIR_11_PENALTY=0.689430
PRIMER_LEFT_11_PENALTY=0.388240
PRIMER_RIGHT_11_PENALTY=0.301190
PRIMER_LEFT_11_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_11_SEQUENCE=TCCGTCTTCCCCAATAAACA
PRIMER_LEFT_11=563,20
PRIMER_RIGHT_11=1199,20
PRIMER_LEFT_11_TM=60.388
PRIMER_RIGHT_11_TM=60.301
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=637
PRIMER_PAIR_12_PENALTY=0.695583
PRIMER_LEFT_12_PENALTY=0.369208
PRIMER_RIGHT_12_PENALTY=0.326376
PRIMER_LEFT_12_SEQUENCE=TTCTCATCATCCACCGTCAG
PRIMER_RIGHT_12_SEQUENCE=GCATTGAAGCCTTATCCGTC
PRIMER_LEFT_12=394,20
PRIMER_RIGHT_12=1213,20
PRIMER_LEFT_12_TM=59.631
PRIMER_RIGHT_12_TM=59.674
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=820
PRIMER_PAIR_13_PENALTY=0.712095
PRIMER_LEFT_13_PENALTY=0.638872
PRIMER_RIGHT_13_PENALTY=0.073222
PRIMER_LEFT_13_SEQUENCE=TTGATGAAGCTCCCTCTTCC
PRIMER_RIGHT_13_SEQUENCE=ACCTTCCCGACTCATCTCCT
PRIMER_LEFT_13=868,20
PRIMER_RIGHT_13=1696,20
PRIMER_LEFT_13_TM=59.361
PRIMER_RIGHT_13_TM=60.073
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=829
PRIMER_PAIR_14_PENALTY=0.714615
PRIMER_LEFT_14_PENALTY=0.388240
PRIMER_RIGHT_14_PENALTY=0.326376
PRIMER_LEFT_14_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_14_SEQUENCE=GCATTGAAGCCTTATCCGTC
PRIMER_LEFT_14=563,20
PRIMER_RIGHT_14=1213,20
PRIMER_LEFT_14_TM=60.388
PRIMER_RIGHT_14_TM=59.674
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=651
PRIMER_PAIR_15_PENALTY=0.735026
PRIMER_LEFT_15_PENALTY=0.580148
PRIMER_RIGHT_15_PENALTY=0.154878
PRIMER_LEFT_15_SEQUENCE=GTGGCAACGAGACAGATGAG
PRIMER_RIGHT_15_SEQUENCE=CCAAAGCGTATGAAGAAGCC
PRIMER_LEFT_15=579,20
PRIMER_RIGHT_15=1547,20
PRIMER_LEFT_15_TM=59.420
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=969
PRIMER_PAIR_16_PENALTY=0.735026
PRIMER_LEFT_16_PENALTY=0.580148
PRIMER_RIGHT_16_PENALTY=0.154878
PRIMER_LEFT_16_SEQUENCE=AGTGGCAACGAGACAGATGA
PRIMER_RIGHT_16_SEQUENCE=CCAAAGCGTATGAAGAAGCC
PRIMER_LEFT_16=578,20
PRIMER_RIGHT_16=1547,20
PRIMER_LEFT_16_TM=59.420
PRIMER_RIGHT_16_TM=59.845
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=970
PRIMER_PAIR_17_PENALTY=0.749619
PRIMER_LEFT_17_PENALTY=0.580148
PRIMER_RIGHT_17_PENALTY=0.169471
PRIMER_LEFT_17_SEQUENCE=GTGGCAACGAGACAGATGAG
PRIMER_RIGHT_17_SEQUENCE=TGAGAGAGTTGCAATGGGTG
PRIMER_LEFT_17=579,20
PRIMER_RIGHT_17=1434,20
PRIMER_LEFT_17_TM=59.420
PRIMER_RIGHT_17_TM=59.831
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=856
PRIMER_PAIR_18_PENALTY=0.749619
PRIMER_LEFT_18_PENALTY=0.580148
PRIMER_RIGHT_18_PENALTY=0.169471
PRIMER_LEFT_18_SEQUENCE=AGTGGCAACGAGACAGATGA
PRIMER_RIGHT_18_SEQUENCE=TGAGAGAGTTGCAATGGGTG
PRIMER_LEFT_18=578,20
PRIMER_RIGHT_18=1434,20
PRIMER_LEFT_18_TM=59.420
PRIMER_RIGHT_18_TM=59.831
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=857
PRIMER_PAIR_19_PENALTY=0.754947
PRIMER_LEFT_19_PENALTY=0.716126
PRIMER_RIGHT_19_PENALTY=0.038821
PRIMER_LEFT_19_SEQUENCE=ATCGTCTTCTTCCGTTGGAG
PRIMER_RIGHT_19_SEQUENCE=GAAGCCTTATCCGTCTTCCC
PRIMER_LEFT_19=556,20
PRIMER_RIGHT_19=1208,20
PRIMER_LEFT_19_TM=59.284
PRIMER_RIGHT_19_TM=60.039
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=653
PRIMER_PAIR_20_PENALTY=0.759304
PRIMER_LEFT_20_PENALTY=0.047584
PRIMER_RIGHT_20_PENALTY=0.711720
PRIMER_LEFT_20_SEQUENCE=TTTCTCATCATCCACCGTCA
PRIMER_RIGHT_20_SEQUENCE=CCGTCTTCCCCAATAAACAC
PRIMER_LEFT_20=393,20
PRIMER_RIGHT_20=1198,20
PRIMER_LEFT_20_TM=60.048
PRIMER_RIGHT_20_TM=59.288
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=806
PRIMER_PAIR_21_PENALTY=0.792033
PRIMER_LEFT_21_PENALTY=0.638872
PRIMER_RIGHT_21_PENALTY=0.153161
PRIMER_LEFT_21_SEQUENCE=TTGATGAAGCTCCCTCTTCC
PRIMER_RIGHT_21_SEQUENCE=TGCAAATCCCCAAATATCGT
PRIMER_LEFT_21=868,20
PRIMER_RIGHT_21=1831,20
PRIMER_LEFT_21_TM=59.361
PRIMER_RIGHT_21_TM=60.153
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=964
PRIMER_PAIR_22_PENALTY=0.793751
PRIMER_LEFT_22_PENALTY=0.638872
PRIMER_RIGHT_22_PENALTY=0.154878
PRIMER_LEFT_22_SEQUENCE=TTGATGAAGCTCCCTCTTCC
PRIMER_RIGHT_22_SEQUENCE=CCAAAGCGTATGAAGAAGCC
PRIMER_LEFT_22=868,20
PRIMER_RIGHT_22=1547,20
PRIMER_LEFT_22_TM=59.361
PRIMER_RIGHT_22_TM=59.845
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=680
PRIMER_PAIR_23_PENALTY=0.806291
PRIMER_LEFT_23_PENALTY=0.580148
PRIMER_RIGHT_23_PENALTY=0.226143
PRIMER_LEFT_23_SEQUENCE=GTGGCAACGAGACAGATGAG
PRIMER_RIGHT_23_SEQUENCE=GCAGAGGATCATTGGTTGCT
PRIMER_LEFT_23=579,20
PRIMER_RIGHT_23=1469,20
PRIMER_LEFT_23_TM=59.420
PRIMER_RIGHT_23_TM=60.226
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=891
PRIMER_PAIR_24_PENALTY=0.806291
PRIMER_LEFT_24_PENALTY=0.580148
PRIMER_RIGHT_24_PENALTY=0.226143
PRIMER_LEFT_24_SEQUENCE=AGTGGCAACGAGACAGATGA
PRIMER_RIGHT_24_SEQUENCE=GCAGAGGATCATTGGTTGCT
PRIMER_LEFT_24=578,20
PRIMER_RIGHT_24=1469,20
PRIMER_LEFT_24_TM=59.420
PRIMER_RIGHT_24_TM=60.226
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=8.5000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=892
PRIMER_PAIR_25_PENALTY=0.808344
PRIMER_LEFT_25_PENALTY=0.638872
PRIMER_RIGHT_25_PENALTY=0.169471
PRIMER_LEFT_25_SEQUENCE=TTGATGAAGCTCCCTCTTCC
PRIMER_RIGHT_25_SEQUENCE=TGAGAGAGTTGCAATGGGTG
PRIMER_LEFT_25=868,20
PRIMER_RIGHT_25=1434,20
PRIMER_LEFT_25_TM=59.361
PRIMER_RIGHT_25_TM=59.831
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=567
PRIMER_PAIR_26_PENALTY=0.842842
PRIMER_LEFT_26_PENALTY=0.388240
PRIMER_RIGHT_26_PENALTY=0.454603
PRIMER_LEFT_26_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_26_SEQUENCE=TGACCTGAACTTGGAGCTGA
PRIMER_LEFT_26=563,20
PRIMER_RIGHT_26=1264,20
PRIMER_LEFT_26_TM=60.388
PRIMER_RIGHT_26_TM=59.545
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=702
PRIMER_PAIR_27_PENALTY=0.845320
PRIMER_LEFT_27_PENALTY=0.772097
PRIMER_RIGHT_27_PENALTY=0.073222
PRIMER_LEFT_27_SEQUENCE=TCCCTCTTCCAAGTCAGGTC
PRIMER_RIGHT_27_SEQUENCE=ACCTTCCCGACTCATCTCCT
PRIMER_LEFT_27=878,20
PRIMER_RIGHT_27=1696,20
PRIMER_LEFT_27_TM=59.228
PRIMER_RIGHT_27_TM=60.073
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=7.9000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=819
PRIMER_PAIR_28_PENALTY=0.847659
PRIMER_LEFT_28_PENALTY=0.808839
PRIMER_RIGHT_28_PENALTY=0.038821
PRIMER_LEFT_28_SEQUENCE=GCTAGTGGCAACGAGACAGA
PRIMER_RIGHT_28_SEQUENCE=GAAGCCTTATCCGTCTTCCC
PRIMER_LEFT_28=575,20
PRIMER_RIGHT_28=1208,20
PRIMER_LEFT_28_TM=59.191
PRIMER_RIGHT_28_TM=60.039
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=634
PRIMER_PAIR_29_PENALTY=0.849105
PRIMER_LEFT_29_PENALTY=0.388240
PRIMER_RIGHT_29_PENALTY=0.460865
PRIMER_LEFT_29_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_29_SEQUENCE=GATCATTGGTTGCTTCAGGG
PRIMER_LEFT_29=563,20
PRIMER_RIGHT_29=1463,20
PRIMER_LEFT_29_TM=60.388
PRIMER_RIGHT_29_TM=60.461
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=901
PRIMER_PAIR_30_PENALTY=0.849105
PRIMER_LEFT_30_PENALTY=0.388240
PRIMER_RIGHT_30_PENALTY=0.460865
PRIMER_LEFT_30_SEQUENCE=TCTTCCGTTGGAGCTAGTGG
PRIMER_RIGHT_30_SEQUENCE=GGATCATTGGTTGCTTCAGG
PRIMER_LEFT_30=563,20
PRIMER_RIGHT_30=1464,20
PRIMER_LEFT_30_TM=60.388
PRIMER_RIGHT_30_TM=60.461
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.9000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=902
PRIMER_PAIR_31_PENALTY=0.864347
PRIMER_LEFT_31_PENALTY=0.638872
PRIMER_RIGHT_31_PENALTY=0.225475
PRIMER_LEFT_31_SEQUENCE=TTGATGAAGCTCCCTCTTCC
PRIMER_RIGHT_31_SEQUENCE=GTTAACCCACCTTCCCGACT
PRIMER_LEFT_31=868,20
PRIMER_RIGHT_31=1704,20
PRIMER_LEFT_31_TM=59.361
PRIMER_RIGHT_31_TM=60.225
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=8.1000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=837
PRIMER_PAIR_32_PENALTY=0.864347
PRIMER_LEFT_32_PENALTY=0.638872
PRIMER_RIGHT_32_PENALTY=0.225475
PRIMER_LEFT_32_SEQUENCE=TTGATGAAGCTCCCTCTTCC
PRIMER_RIGHT_32_SEQUENCE=AGTTAACCCACCTTCCCGAC
PRIMER_LEFT_32=868,20
PRIMER_RIGHT_32=1705,20
PRIMER_LEFT_32_TM=59.361
PRIMER_RIGHT_32_TM=60.225
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=838
PRIMER_PAIR_33_PENALTY=0.865016
PRIMER_LEFT_33_PENALTY=0.638872
PRIMER_RIGHT_33_PENALTY=0.226143
PRIMER_LEFT_33_SEQUENCE=TTGATGAAGCTCCCTCTTCC
PRIMER_RIGHT_33_SEQUENCE=GCAGAGGATCATTGGTTGCT
PRIMER_LEFT_33=868,20
PRIMER_RIGHT_33=1469,20
PRIMER_LEFT_33_TM=59.361
PRIMER_RIGHT_33_TM=60.226
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=8.5000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=602
PRIMER_PAIR_34_PENALTY=0.881338
PRIMER_LEFT_34_PENALTY=0.580148
PRIMER_RIGHT_34_PENALTY=0.301190
PRIMER_LEFT_34_SEQUENCE=GTGGCAACGAGACAGATGAG
PRIMER_RIGHT_34_SEQUENCE=TCCGTCTTCCCCAATAAACA
PRIMER_LEFT_34=579,20
PRIMER_RIGHT_34=1199,20
PRIMER_LEFT_34_TM=59.420
PRIMER_RIGHT_34_TM=60.301
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.6000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=621
PRIMER_PAIR_35_PENALTY=0.881338
PRIMER_LEFT_35_PENALTY=0.580148
PRIMER_RIGHT_35_PENALTY=0.301190
PRIMER_LEFT_35_SEQUENCE=AGTGGCAACGAGACAGATGA
PRIMER_RIGHT_35_SEQUENCE=TCCGTCTTCCCCAATAAACA
PRIMER_LEFT_35=578,20
PRIMER_RIGHT_35=1199,20
PRIMER_LEFT_35_TM=59.420
PRIMER_RIGHT_35_TM=60.301
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.6000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=622
PRIMER_PAIR_36_PENALTY=0.885598
PRIMER_LEFT_36_PENALTY=0.716126
PRIMER_RIGHT_36_PENALTY=0.169471
PRIMER_LEFT_36_SEQUENCE=ATCGTCTTCTTCCGTTGGAG
PRIMER_RIGHT_36_SEQUENCE=TGAGAGAGTTGCAATGGGTG
PRIMER_LEFT_36=556,20
PRIMER_RIGHT_36=1434,20
PRIMER_LEFT_36_TM=59.284
PRIMER_RIGHT_36_TM=59.831
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=879
PRIMER_PAIR_37_PENALTY=0.897012
PRIMER_LEFT_37_PENALTY=0.595822
PRIMER_RIGHT_37_PENALTY=0.301190
PRIMER_LEFT_37_SEQUENCE=ATCCTCCGTCTTCTTCCGAC
PRIMER_RIGHT_37_SEQUENCE=TCCGTCTTCCCCAATAAACA
PRIMER_LEFT_37=228,20
PRIMER_RIGHT_37=1199,20
PRIMER_LEFT_37_TM=60.596
PRIMER_RIGHT_37_TM=60.301
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=972
PRIMER_PAIR_38_PENALTY=0.922198
PRIMER_LEFT_38_PENALTY=0.595822
PRIMER_RIGHT_38_PENALTY=0.326376
PRIMER_LEFT_38_SEQUENCE=ATCCTCCGTCTTCTTCCGAC
PRIMER_RIGHT_38_SEQUENCE=GCATTGAAGCCTTATCCGTC
PRIMER_LEFT_38=228,20
PRIMER_RIGHT_38=1213,20
PRIMER_LEFT_38_TM=60.596
PRIMER_RIGHT_38_TM=59.674
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=986
PRIMER_PAIR_39_PENALTY=0.925258
PRIMER_LEFT_39_PENALTY=0.772097
PRIMER_RIGHT_39_PENALTY=0.153161
PRIMER_LEFT_39_SEQUENCE=TCCCTCTTCCAAGTCAGGTC
PRIMER_RIGHT_39_SEQUENCE=TGCAAATCCCCAAATATCGT
PRIMER_LEFT_39=878,20
PRIMER_RIGHT_39=1831,20
PRIMER_LEFT_39_TM=59.228
PRIMER_RIGHT_39_TM=60.153
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=8.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=954
PRIMER_PAIR_40_PENALTY=0.926976
PRIMER_LEFT_40_PENALTY=0.772097
PRIMER_RIGHT_40_PENALTY=0.154878
PRIMER_LEFT_40_SEQUENCE=TCCCTCTTCCAAGTCAGGTC
PRIMER_RIGHT_40_SEQUENCE=CCAAAGCGTATGAAGAAGCC
PRIMER_LEFT_40=878,20
PRIMER_RIGHT_40=1547,20
PRIMER_LEFT_40_TM=59.228
PRIMER_RIGHT_40_TM=59.845
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.6000
PRIMER_RIGHT_40_END_STABILITY=9.7000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=670
PRIMER_PAIR_41_PENALTY=0.941569
PRIMER_LEFT_41_PENALTY=0.772097
PRIMER_RIGHT_41_PENALTY=0.169471
PRIMER_LEFT_41_SEQUENCE=TCCCTCTTCCAAGTCAGGTC
PRIMER_RIGHT_41_SEQUENCE=TGAGAGAGTTGCAATGGGTG
PRIMER_LEFT_41=878,20
PRIMER_RIGHT_41=1434,20
PRIMER_LEFT_41_TM=59.228
PRIMER_RIGHT_41_TM=59.831
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.6000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=557
PRIMER_PAIR_42_PENALTY=0.942269
PRIMER_LEFT_42_PENALTY=0.716126
PRIMER_RIGHT_42_PENALTY=0.226143
PRIMER_LEFT_42_SEQUENCE=ATCGTCTTCTTCCGTTGGAG
PRIMER_RIGHT_42_SEQUENCE=GCAGAGGATCATTGGTTGCT
PRIMER_LEFT_42=556,20
PRIMER_RIGHT_42=1469,20
PRIMER_LEFT_42_TM=59.284
PRIMER_RIGHT_42_TM=60.226
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=914
PRIMER_PAIR_43_PENALTY=0.954756
PRIMER_LEFT_43_PENALTY=0.881533
PRIMER_RIGHT_43_PENALTY=0.073222
PRIMER_LEFT_43_SEQUENCE=TGATGAAGCTCCCTCTTCCA
PRIMER_RIGHT_43_SEQUENCE=ACCTTCCCGACTCATCTCCT
PRIMER_LEFT_43=869,20
PRIMER_RIGHT_43=1696,20
PRIMER_LEFT_43_TM=60.882
PRIMER_RIGHT_43_TM=60.073
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=828
PRIMER_PAIR_44_PENALTY=0.963717
PRIMER_LEFT_44_PENALTY=0.808839
PRIMER_RIGHT_44_PENALTY=0.154878
PRIMER_LEFT_44_SEQUENCE=GCTAGTGGCAACGAGACAGA
PRIMER_RIGHT_44_SEQUENCE=CCAAAGCGTATGAAGAAGCC
PRIMER_LEFT_44=575,20
PRIMER_RIGHT_44=1547,20
PRIMER_LEFT_44_TM=59.191
PRIMER_RIGHT_44_TM=59.845
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=9.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=973
PRIMER_PAIR_45_PENALTY=0.978310
PRIMER_LEFT_45_PENALTY=0.808839
PRIMER_RIGHT_45_PENALTY=0.169471
PRIMER_LEFT_45_SEQUENCE=GCTAGTGGCAACGAGACAGA
PRIMER_RIGHT_45_SEQUENCE=TGAGAGAGTTGCAATGGGTG
PRIMER_LEFT_45=575,20
PRIMER_RIGHT_45=1434,20
PRIMER_LEFT_45_TM=59.191
PRIMER_RIGHT_45_TM=59.831
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=860
PRIMER_PAIR_46_PENALTY=0.997572
PRIMER_LEFT_46_PENALTY=0.772097
PRIMER_RIGHT_46_PENALTY=0.225475
PRIMER_LEFT_46_SEQUENCE=TCCCTCTTCCAAGTCAGGTC
PRIMER_RIGHT_46_SEQUENCE=GTTAACCCACCTTCCCGACT
PRIMER_LEFT_46=878,20
PRIMER_RIGHT_46=1704,20
PRIMER_LEFT_46_TM=59.228
PRIMER_RIGHT_46_TM=60.225
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.6000
PRIMER_RIGHT_46_END_STABILITY=8.1000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=827
PRIMER_PAIR_47_PENALTY=0.997572
PRIMER_LEFT_47_PENALTY=0.772097
PRIMER_RIGHT_47_PENALTY=0.225475
PRIMER_LEFT_47_SEQUENCE=TCCCTCTTCCAAGTCAGGTC
PRIMER_RIGHT_47_SEQUENCE=AGTTAACCCACCTTCCCGAC
PRIMER_LEFT_47=878,20
PRIMER_RIGHT_47=1705,20
PRIMER_LEFT_47_TM=59.228
PRIMER_RIGHT_47_TM=60.225
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.6000
PRIMER_RIGHT_47_END_STABILITY=9.6000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=828
PRIMER_PAIR_48_PENALTY=0.998241
PRIMER_LEFT_48_PENALTY=0.772097
PRIMER_RIGHT_48_PENALTY=0.226143
PRIMER_LEFT_48_SEQUENCE=TCCCTCTTCCAAGTCAGGTC
PRIMER_RIGHT_48_SEQUENCE=GCAGAGGATCATTGGTTGCT
PRIMER_LEFT_48=878,20
PRIMER_RIGHT_48=1469,20
PRIMER_LEFT_48_TM=59.228
PRIMER_RIGHT_48_TM=60.226
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.6000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=592
PRIMER_PAIR_49_PENALTY=1.017316
PRIMER_LEFT_49_PENALTY=0.716126
PRIMER_RIGHT_49_PENALTY=0.301190
PRIMER_LEFT_49_SEQUENCE=ATCGTCTTCTTCCGTTGGAG
PRIMER_RIGHT_49_SEQUENCE=TCCGTCTTCCCCAATAAACA
PRIMER_LEFT_49=556,20
PRIMER_RIGHT_49=1199,20
PRIMER_LEFT_49_TM=59.284
PRIMER_RIGHT_49_TM=60.301
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=644
=
SEQUENCE_ID=MAGIC.149_4_4504172_4504394_ct-1_ler-0_1248_70086_TRUE
SEQUENCE_TARGET=950,322
SEQUENCE_TEMPLATE=NCATTTTTANGATNAATTCNNAAANTGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAANGANNTTTGCACNCGNNNGAATGANATNGCCGNNCCNCANTANCANTCCACNTGNACNCTNCAANCCCTNAATNGATTGNGAGAGCTNTGTCGNNNNNNNNNNNNNANTATGAGGGANTCNCANATGCCNNANCCNANAGANTCGCCGNAAGACCANNNTNCNGGGTTCTTCTNCNTGTNCGANATNTANTTCAAGGGTTGCGGTNTGANNTTTNCNCTTCCNGNAGCCCTNGTTCNGTACTTANCNGCCCTNNAAATTGCCNTGCCCNNNNTNACCCCTNACNTNCTNCGNNCTATNTTAGNGATTATTACNATCGCNNCNNANGCCGGATNTGTNATCNGNGTTCCNNAACTGAATGAACTTCTCAGCNTGAGGAGTTCTTCNAAGAAAGNNGNATACTTCTNGGCTTATNCGAANGCCNACANGAANCTCATCTNNNATCTTNCNAACAANGACGANAACTNGCANCANCCNTGGTTNNTAANNNNNNNNNNNNNNNNNNNNNTNGNAAATCTTTCCGACNTGCTCCCCNNGAAGNCNNGTAGNNCTTAGGTCATGCNTATGATTTTTATANANATNTTGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGAAACTCTTTGGAATTCCTTCACCNTNGACNGNTTCATANAGGCNAACCACAAGCTCANGANGAGTCNCCCTGNACAACTTCTTCAACTTCNGTTNCCTCCTCCTTTACCTCANNGANCNTCNNCTCGAGNCATCGCCNCCNGANGNAAGANGNTGTCCAAATCNATCGTGGAGGCTTGTGANGNGAACAANTNNTTCNTTACGTNGGNTNTNGANAAGAAGGAGTACAGNAGAACCCNGNTANTNNANGNTGGNAGTNCCNAAGGNGNNAGATCNGNNNNTGCCTTCAGACCCANNCCTCANNNTGAAGGTCATGGTNGNNNNTCACCTCNNNGAGACNNATCACCTCNTCGTGANTCACCTNNGCCTNCNTNTNGNAATAGGTTGAGCAGNGAANCNATCNCGNANTTNNTTNNNAAGAANAGNNAAANNCCANCNCNNNGCAGTTNNTCNNCTAAGAGNGATAAGACNANAGCCNGAACNGATCGTTCTCCTCNATCNTCTCCCCCACCTNGCTNGATGGNTNCCNNTCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTNCAAATCCTCGACANGAAGANGNNGAGNCCCGAGATNCTANCCCTAAACCCAAGGNCNNTNTGGTTNCTCAAACNAAATCACTCACTNGTNAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACAGCGATGNAAACAGAGGGAANNNNNNNNNNATGCTTCAAGACTAGGAAAGACACGANCTNNCCAACCTTTGANANGTGGCGTCCTGCGATTCNNGAGNGATTCNTACTTCNNGCNCATCACTCNTCTCGGGNNNNNNNNNNNNNNNNNNNNNNNNTNANCTCNACTCTTTNGCTTATACNCNAACTCACCTTTGTAANCNTTGNTTCAGGCAAACANCNNNCTCAATGACATGATTGAGTATTACGAAAGNNTGNTGCTTGNCANGGAANGANGTTATNGCCTNGAAAGANAAGTTTTCTTCTNTTGAGTCCGATCTCNNCTCTTCCNCTGAAGTCANGCNNAAACTNGAAGANCAATTGGACAANCTTTNTTCCGAACTCANNNNNNNNNNNNNGAGTTGNAGGNTCAGTATCANNGGTATGANAANATCNAGGAGGAACTCTCGAATGCTCNNGNCAGACTNTNAGNNNNNNNNNNNNNNNNTTACGACTTGTCCAACCAGCTATCCNAANTGCANGCCANNTACAAAGCAATNGCCAAACNTCGAGATGNTNAGTTANNNNNNNNNNNNNNAAAAGCCAGGAAAGANGTAAANGGTCGCGGGATGGAANTGATTCAANGGGCNATCGTNTTNATCNAAACCGAGAAAACTAGGACGNANNTGGAATCAGANATCAAGGANTATGAAATCAACCTCCTCCTTCTGNGNCAAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_38517_38530_rsch-4_wil-2_28638_164114_TRUE
SEQUENCE_TARGET=950,113
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14897033_14897714_po-0_kn-0_119060_12376_TRUE
SEQUENCE_TARGET=950,781
SEQUENCE_TEMPLATE=AAATATTAGTATTTCGGTTTAGANTAGAACAAATANGAGACGATNNANNNNNTGANAACAAANAGCTATNTATGTTGTGTTTCATATCTTATCAACTCGGTCNCNTCCTCTTTCCATTGGATGNAATTCTTTTTCATAGAAGAACCACTANTTCTTTTNTTTCTTTTGCCATTAACNCAANAATTTTTATTGTANTTAGTGGTTNNNNCTTATGTTTNTTTGCCTTNGAATNTAATCACTCTNTTTTNANNAANAANANAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGAATGTAATCACTCTTAAAGTGATCTNTNNNNATTTGAAAAAGTGTCATATTTATTAGCTATAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAATTAAANTTATAACGAATTACTATGNNNNNNNNNNNNNNNAACNATCAAATTAAACGAGTTAAATTATNCTTNTCATGTCTGCCTGTAATGATAANATAATATATATCAAANCTTNTGAAACTTCNNTTTTTTTTNTTTTNNGTTGTCATTGCNAAGNTTAGATTAATAACAATATCACTGATAATGATGCTTACACGTAAACCTAAACAAANGAGGAAAANAAACTAANTCTTACACGTAAACCTTAACCGATNCAGCATAGGTNTTGACAATATTTTCTTTCCTTTTATTTAAATAGACGACGTTGAGATNTTTAANATTGTCATCATTTATAACCATNTTTTAAGTTGTCTTTATCTGAAATATACAAATGTTCAATTNGTACATTGACCTAAAAGTCTNTNGGACTAATGATTGATTAGNGGTTNATTNTGNNNNNNNNNNNNNTATGTTGTATGGAACNAGAAGCAANATTGTTTTAAACTCAATGTCATTAANTGAATTAGAGAAAGANCATCAAAANTCTTAAACTATATCTCCCAAAANTANCTTAATTCAGTTTGAANANAAAAAGCCCACAAAGCCCAAAACTCACTCNTTCATAAGCCNATTATAATATCCNAAATNAAAGCCNATNAAGCCCAAAACNCANTCATTCGTGTCTCCTTCTCTCTCGCACAAATGCTTCAGGAGTTCTCACAACAAGATCGAGAAATACACACATAGAAGACNNNGAAGAAGAAGAAGACGATACAGATCCACAACGCNATGGGTTTAGGCATCTTAGCTTCTCGAACNATTCGACCAGCTTCNCGTCTTCTTCAATCTCAAACCTCCAATTTCTTCCTCCGCACAATCGTCTCAAAACCAGANCTCCAATCTCCTGAATCCGCTGCTGTCTCTGAGCCTGAGCCNCCGACACAGATTCTCCCTCCTAGAAACCCTGTAGGTGGAGCACGCGTCCANTTCTCGAATCCTGAAGATGCGATCGANGTNTTNGTNGATGGTTACGCGGTCAAAGTNCCTAAAGGATTCACTGTTCTTCAGGCTTGTGAGGTCGCTGGAGTTGATATCCCGAGGTTTTGTTATCATTCTCGTTTNTCTATTGCTGGAAATTGCAGGATGTGTCTTGTTGAGGTTGAGAAATCTCCTAAGCCTGTAGCNTCATGTGCCATGCCTGCNCTTCCTGGTNAGTTCGATTNCAGGAAANTTTCTATGCTGATGGATTGATTTTGATCGTTNATTGGTNGAANANATTTGGATTTGGTGCTATTGCTAAGAGTTTTTGTAACAGGGATGAAGATTAAGACTGATACACCNATAGCNAAGAAGGCGAGGGAGGGAGTGATGGAGTTTTTGTTGATGAATCANCCTTTGGATTGNCCTATTTGTGACCAAGGAGGAGAGTGTGATCTTCAGGATCAGTCTATGGCTTTTGGATCTGATAGAGGACGTTTTACTGAGATGAAAAGATCTGTTGTTGATAAGAATCTTGGTCCTCTTGTGAAGACTGTTATGACTCGGTGTATCCAGTGTACAAGGTTACTAATATAGTTTGCTTANAGTGTGATGTTTTAGTTAATGTGATAGATANGCTATCCTGTTGATTAGGTTTTGTATGTATAATGTTTTTGTAGGTGTGTACGATTTGCTTCTGAAGTTGCTGGGGTTCAGGATCTTGGTATACTAGGTCGTGGTAGTGGAGANGAAATTGGAACTTATGTTGAAAAGCTTATGACCAGTGAACTCTCTGGAAATGTTATTGATATCTGTCCTGTNGGAGCCTTGACNTCAAAGCCNTTTGCCTTTAAAGCCAGAAACTGGGAGTTGAAAGCAACAGAAACTATTGATGTTAGTGATGCNGTNGGGTCCAACATNCGTGTTGANAGTAGAGGACCAGAAGTNATGCGTATCATTCCACGCCTNAATGAGGTATGTNATGCTTTTGTTNNNNNNNNNNNNNNNNNNNNGTATTTTGCTGGATGTTGAACT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=6.225392
PRIMER_LEFT_0_PENALTY=5.781271
PRIMER_RIGHT_0_PENALTY=0.444121
PRIMER_LEFT_0_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_0_SEQUENCE=AACTCCAGCGACCTCACAAG
PRIMER_LEFT_0=919,22
PRIMER_RIGHT_0=1756,20
PRIMER_LEFT_0_TM=56.219
PRIMER_RIGHT_0_TM=60.444
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=838
PRIMER_PAIR_1_PENALTY=6.268511
PRIMER_LEFT_1_PENALTY=5.781271
PRIMER_RIGHT_1_PENALTY=0.487240
PRIMER_LEFT_1_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_1_SEQUENCE=GGATATCAACTCCAGCGACC
PRIMER_LEFT_1=919,22
PRIMER_RIGHT_1=1763,20
PRIMER_LEFT_1_TM=56.219
PRIMER_RIGHT_1_TM=59.513
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=845
PRIMER_PAIR_2_PENALTY=6.268511
PRIMER_LEFT_2_PENALTY=5.781271
PRIMER_RIGHT_2_PENALTY=0.487240
PRIMER_LEFT_2_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_2_SEQUENCE=GGGATATCAACTCCAGCGAC
PRIMER_LEFT_2=919,22
PRIMER_RIGHT_2=1764,20
PRIMER_LEFT_2_TM=56.219
PRIMER_RIGHT_2_TM=59.513
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=846
PRIMER_PAIR_3_PENALTY=6.386886
PRIMER_LEFT_3_PENALTY=5.781271
PRIMER_RIGHT_3_PENALTY=0.605616
PRIMER_LEFT_3_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_3_SEQUENCE=TATCAACTCCAGCGACCTCA
PRIMER_LEFT_3=919,22
PRIMER_RIGHT_3=1760,20
PRIMER_LEFT_3_TM=56.219
PRIMER_RIGHT_3_TM=59.394
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=842
PRIMER_PAIR_4_PENALTY=6.420143
PRIMER_LEFT_4_PENALTY=5.781271
PRIMER_RIGHT_4_PENALTY=0.638872
PRIMER_LEFT_4_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_4_SEQUENCE=ACCTCGGGATATCAACTCCA
PRIMER_LEFT_4=919,22
PRIMER_RIGHT_4=1769,20
PRIMER_LEFT_4_TM=56.219
PRIMER_RIGHT_4_TM=59.361
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=851
PRIMER_PAIR_5_PENALTY=7.072798
PRIMER_LEFT_5_PENALTY=5.781271
PRIMER_RIGHT_5_PENALTY=1.291527
PRIMER_LEFT_5_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_5_SEQUENCE=ATATCAACTCCAGCGACCTCA
PRIMER_LEFT_5=919,22
PRIMER_RIGHT_5=1761,21
PRIMER_LEFT_5_TM=56.219
PRIMER_RIGHT_5_TM=59.708
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=843
PRIMER_PAIR_6_PENALTY=7.259642
PRIMER_LEFT_6_PENALTY=5.781271
PRIMER_RIGHT_6_PENALTY=1.478371
PRIMER_LEFT_6_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_6_SEQUENCE=GGATATCAACTCCAGCGACCT
PRIMER_LEFT_6=919,22
PRIMER_RIGHT_6=1763,21
PRIMER_LEFT_6_TM=56.219
PRIMER_RIGHT_6_TM=60.478
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=845
PRIMER_PAIR_7_PENALTY=7.479269
PRIMER_LEFT_7_PENALTY=5.781271
PRIMER_RIGHT_7_PENALTY=1.697998
PRIMER_LEFT_7_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_7_SEQUENCE=AACCTCGGGATATCAACTCCA
PRIMER_LEFT_7=919,22
PRIMER_RIGHT_7=1770,21
PRIMER_LEFT_7_TM=56.219
PRIMER_RIGHT_7_TM=60.698
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=852
PRIMER_PAIR_8_PENALTY=7.793741
PRIMER_LEFT_8_PENALTY=5.781271
PRIMER_RIGHT_8_PENALTY=2.012470
PRIMER_LEFT_8_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_8_SEQUENCE=TCAACCTCAACAAGACACATCC
PRIMER_LEFT_8=919,22
PRIMER_RIGHT_8=1830,22
PRIMER_LEFT_8_TM=56.219
PRIMER_RIGHT_8_TM=60.012
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=912
PRIMER_PAIR_9_PENALTY=7.945910
PRIMER_LEFT_9_PENALTY=5.781271
PRIMER_RIGHT_9_PENALTY=2.164639
PRIMER_LEFT_9_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_9_SEQUENCE=CAAAACCTCGGGATATCAACTC
PRIMER_LEFT_9=919,22
PRIMER_RIGHT_9=1773,22
PRIMER_LEFT_9_TM=56.219
PRIMER_RIGHT_9_TM=59.835
PRIMER_LEFT_9_GC_PERCENT=40.909
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=855
PRIMER_PAIR_10_PENALTY=8.195796
PRIMER_LEFT_10_PENALTY=5.781271
PRIMER_RIGHT_10_PENALTY=2.414525
PRIMER_LEFT_10_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_10_SEQUENCE=CAACTCCAGCGACCTCACAA
PRIMER_LEFT_10=919,22
PRIMER_RIGHT_10=1757,20
PRIMER_LEFT_10_TM=56.219
PRIMER_RIGHT_10_TM=62.415
PRIMER_LEFT_10_GC_PERCENT=40.909
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=2.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=839
PRIMER_PAIR_11_PENALTY=8.669615
PRIMER_LEFT_11_PENALTY=5.781271
PRIMER_RIGHT_11_PENALTY=2.888344
PRIMER_LEFT_11_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_11_SEQUENCE=CAACCTCAACAAGACACATCCT
PRIMER_LEFT_11=919,22
PRIMER_RIGHT_11=1829,22
PRIMER_LEFT_11_TM=56.219
PRIMER_RIGHT_11_TM=59.112
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.6000
PRIMER_RIGHT_11_END_STABILITY=7.8000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=911
PRIMER_PAIR_12_PENALTY=8.722254
PRIMER_LEFT_12_PENALTY=5.781271
PRIMER_RIGHT_12_PENALTY=2.940983
PRIMER_LEFT_12_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_12_SEQUENCE=CAAAACCTCGGGATATCAACT
PRIMER_LEFT_12=919,22
PRIMER_RIGHT_12=1773,21
PRIMER_LEFT_12_TM=56.219
PRIMER_RIGHT_12_TM=58.059
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=855
PRIMER_PAIR_13_PENALTY=8.737306
PRIMER_LEFT_13_PENALTY=5.781271
PRIMER_RIGHT_13_PENALTY=2.956036
PRIMER_LEFT_13_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_13_SEQUENCE=CAACCTCAACAAGACACATCC
PRIMER_LEFT_13=919,22
PRIMER_RIGHT_13=1829,21
PRIMER_LEFT_13_TM=56.219
PRIMER_RIGHT_13_TM=58.044
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=911
PRIMER_PAIR_14_PENALTY=8.823551
PRIMER_LEFT_14_PENALTY=5.781271
PRIMER_RIGHT_14_PENALTY=3.042280
PRIMER_LEFT_14_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_14_SEQUENCE=TCAACTCCAGCGACCTCACA
PRIMER_LEFT_14=919,22
PRIMER_RIGHT_14=1758,20
PRIMER_LEFT_14_TM=56.219
PRIMER_RIGHT_14_TM=63.042
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=840
PRIMER_PAIR_15_PENALTY=8.887717
PRIMER_LEFT_15_PENALTY=5.781271
PRIMER_RIGHT_15_PENALTY=3.106446
PRIMER_LEFT_15_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_15_SEQUENCE=ACAAAACCTCGGGATATCAACT
PRIMER_LEFT_15=919,22
PRIMER_RIGHT_15=1774,22
PRIMER_LEFT_15_TM=56.219
PRIMER_RIGHT_15_TM=58.894
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=856
PRIMER_PAIR_16_PENALTY=8.994370
PRIMER_LEFT_16_PENALTY=5.781271
PRIMER_RIGHT_16_PENALTY=3.213099
PRIMER_LEFT_16_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_16_SEQUENCE=GATATCAACTCCAGCGACCT
PRIMER_LEFT_16=919,22
PRIMER_RIGHT_16=1762,20
PRIMER_LEFT_16_TM=56.219
PRIMER_RIGHT_16_TM=56.787
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=2.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=844
PRIMER_PAIR_17_PENALTY=9.143816
PRIMER_LEFT_17_PENALTY=5.781271
PRIMER_RIGHT_17_PENALTY=3.362545
PRIMER_LEFT_17_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_17_SEQUENCE=CGGGATATCAACTCCAGCGA
PRIMER_LEFT_17=919,22
PRIMER_RIGHT_17=1765,20
PRIMER_LEFT_17_TM=56.219
PRIMER_RIGHT_17_TM=63.363
PRIMER_LEFT_17_GC_PERCENT=40.909
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=9.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=847
PRIMER_PAIR_18_PENALTY=9.183471
PRIMER_LEFT_18_PENALTY=5.781271
PRIMER_RIGHT_18_PENALTY=3.402201
PRIMER_LEFT_18_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_18_SEQUENCE=AGCGACCTCACAAGCCTGAA
PRIMER_LEFT_18=919,22
PRIMER_RIGHT_18=1750,20
PRIMER_LEFT_18_TM=56.219
PRIMER_RIGHT_18_TM=63.402
PRIMER_LEFT_18_GC_PERCENT=40.909
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.6000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=832
PRIMER_PAIR_19_PENALTY=9.314476
PRIMER_LEFT_19_PENALTY=5.781271
PRIMER_RIGHT_19_PENALTY=3.533205
PRIMER_LEFT_19_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_19_SEQUENCE=GATATCAACTCCAGCGACCTCA
PRIMER_LEFT_19=919,22
PRIMER_RIGHT_19=1762,22
PRIMER_LEFT_19_TM=56.219
PRIMER_RIGHT_19_TM=61.533
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=2.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=844
PRIMER_PAIR_20_PENALTY=9.365208
PRIMER_LEFT_20_PENALTY=5.781271
PRIMER_RIGHT_20_PENALTY=3.583938
PRIMER_LEFT_20_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_20_SEQUENCE=ACAAAACCTCGGGATATCAACTC
PRIMER_LEFT_20=919,22
PRIMER_RIGHT_20=1774,23
PRIMER_LEFT_20_TM=56.219
PRIMER_RIGHT_20_TM=60.584
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=43.478
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.6000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=856
PRIMER_PAIR_21_PENALTY=9.384091
PRIMER_LEFT_21_PENALTY=5.781271
PRIMER_RIGHT_21_PENALTY=3.602821
PRIMER_LEFT_21_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_21_SEQUENCE=TTCTCAACCTCAACAAGACACA
PRIMER_LEFT_21=919,22
PRIMER_RIGHT_21=1833,22
PRIMER_LEFT_21_TM=56.219
PRIMER_RIGHT_21_TM=58.397
PRIMER_LEFT_21_GC_PERCENT=40.909
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.6000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=915
PRIMER_PAIR_22_PENALTY=9.488689
PRIMER_LEFT_22_PENALTY=5.781271
PRIMER_RIGHT_22_PENALTY=3.707418
PRIMER_LEFT_22_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_22_SEQUENCE=GGAGATTTCTCAACCTCAACAA
PRIMER_LEFT_22=919,22
PRIMER_RIGHT_22=1839,22
PRIMER_LEFT_22_TM=56.219
PRIMER_RIGHT_22_TM=58.293
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=40.909
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=921
PRIMER_PAIR_23_PENALTY=9.512796
PRIMER_LEFT_23_PENALTY=5.781271
PRIMER_RIGHT_23_PENALTY=3.731526
PRIMER_LEFT_23_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_23_SEQUENCE=GGAGATTTCTCAACCTCAACAAG
PRIMER_LEFT_23=919,22
PRIMER_RIGHT_23=1839,23
PRIMER_LEFT_23_TM=56.219
PRIMER_RIGHT_23_TM=59.268
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=43.478
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.6000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=921
PRIMER_PAIR_24_PENALTY=9.534629
PRIMER_LEFT_24_PENALTY=5.781271
PRIMER_RIGHT_24_PENALTY=3.753359
PRIMER_LEFT_24_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_24_SEQUENCE=TCTCAACCTCAACAAGACACATC
PRIMER_LEFT_24=919,22
PRIMER_RIGHT_24=1832,23
PRIMER_LEFT_24_TM=56.219
PRIMER_RIGHT_24_TM=59.247
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=43.478
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.6000
PRIMER_RIGHT_24_END_STABILITY=6.3000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=914
PRIMER_PAIR_25_PENALTY=9.620424
PRIMER_LEFT_25_PENALTY=5.781271
PRIMER_RIGHT_25_PENALTY=3.839153
PRIMER_LEFT_25_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_25_SEQUENCE=AAAACCTCGGGATATCAACTC
PRIMER_LEFT_25=919,22
PRIMER_RIGHT_25=1772,21
PRIMER_LEFT_25_TM=56.219
PRIMER_RIGHT_25_TM=57.161
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=9.6000
PRIMER_RIGHT_25_END_STABILITY=6.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=854
PRIMER_PAIR_26_PENALTY=9.680720
PRIMER_LEFT_26_PENALTY=5.781271
PRIMER_RIGHT_26_PENALTY=3.899449
PRIMER_LEFT_26_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_26_SEQUENCE=AAACCTCGGGATATCAACTCCA
PRIMER_LEFT_26=919,22
PRIMER_RIGHT_26=1771,22
PRIMER_LEFT_26_TM=56.219
PRIMER_RIGHT_26_TM=61.899
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.6000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=853
PRIMER_PAIR_27_PENALTY=9.737354
PRIMER_LEFT_27_PENALTY=5.781271
PRIMER_RIGHT_27_PENALTY=3.956083
PRIMER_LEFT_27_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_27_SEQUENCE=TCAACCTCAACAAGACACATCCT
PRIMER_LEFT_27=919,22
PRIMER_RIGHT_27=1830,23
PRIMER_LEFT_27_TM=56.219
PRIMER_RIGHT_27_TM=60.956
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.6000
PRIMER_RIGHT_27_END_STABILITY=7.8000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=912
PRIMER_PAIR_28_PENALTY=9.737354
PRIMER_LEFT_28_PENALTY=5.781271
PRIMER_RIGHT_28_PENALTY=3.956083
PRIMER_LEFT_28_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_28_SEQUENCE=CTCAACCTCAACAAGACACATCC
PRIMER_LEFT_28=919,22
PRIMER_RIGHT_28=1831,23
PRIMER_LEFT_28_TM=56.219
PRIMER_RIGHT_28_TM=60.956
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=47.826
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.6000
PRIMER_RIGHT_28_END_STABILITY=8.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=913
PRIMER_PAIR_29_PENALTY=9.777973
PRIMER_LEFT_29_PENALTY=5.781271
PRIMER_RIGHT_29_PENALTY=3.996703
PRIMER_LEFT_29_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_29_SEQUENCE=GGGATATCAACTCCAGCGACC
PRIMER_LEFT_29=919,22
PRIMER_RIGHT_29=1764,21
PRIMER_LEFT_29_TM=56.219
PRIMER_RIGHT_29_TM=62.997
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=57.143
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.6000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=846
PRIMER_PAIR_30_PENALTY=9.872136
PRIMER_LEFT_30_PENALTY=5.781271
PRIMER_RIGHT_30_PENALTY=4.090865
PRIMER_LEFT_30_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_30_SEQUENCE=AGGAGATTTCTCAACCTCAACA
PRIMER_LEFT_30=919,22
PRIMER_RIGHT_30=1840,22
PRIMER_LEFT_30_TM=56.219
PRIMER_RIGHT_30_TM=57.909
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=922
PRIMER_PAIR_31_PENALTY=9.939145
PRIMER_LEFT_31_PENALTY=5.781271
PRIMER_RIGHT_31_PENALTY=4.157874
PRIMER_LEFT_31_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_31_SEQUENCE=ATCAACTCCAGCGACCTCACA
PRIMER_LEFT_31=919,22
PRIMER_RIGHT_31=1759,21
PRIMER_LEFT_31_TM=56.219
PRIMER_RIGHT_31_TM=63.158
PRIMER_LEFT_31_GC_PERCENT=40.909
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.6000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=2.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=841
PRIMER_PAIR_32_PENALTY=9.942593
PRIMER_LEFT_32_PENALTY=5.781271
PRIMER_RIGHT_32_PENALTY=4.161322
PRIMER_LEFT_32_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_32_SEQUENCE=AGGAGATTTCTCAACCTCAACAAG
PRIMER_LEFT_32=919,22
PRIMER_RIGHT_32=1840,24
PRIMER_LEFT_32_TM=56.219
PRIMER_RIGHT_32_TM=60.161
PRIMER_LEFT_32_GC_PERCENT=40.909
PRIMER_RIGHT_32_GC_PERCENT=41.667
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.6000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=922
PRIMER_PAIR_33_PENALTY=9.962567
PRIMER_LEFT_33_PENALTY=5.781271
PRIMER_RIGHT_33_PENALTY=4.181296
PRIMER_LEFT_33_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_33_SEQUENCE=TCTCAACCTCAACAAGACACA
PRIMER_LEFT_33=919,22
PRIMER_RIGHT_33=1832,21
PRIMER_LEFT_33_TM=56.219
PRIMER_RIGHT_33_TM=56.819
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.6000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=914
PRIMER_PAIR_34_PENALTY=9.970166
PRIMER_LEFT_34_PENALTY=5.781271
PRIMER_RIGHT_34_PENALTY=4.188895
PRIMER_LEFT_34_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_34_SEQUENCE=GGAGATTTCTCAACCTCAACA
PRIMER_LEFT_34=919,22
PRIMER_RIGHT_34=1839,21
PRIMER_LEFT_34_TM=56.219
PRIMER_RIGHT_34_TM=56.811
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.6000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=921
PRIMER_PAIR_35_PENALTY=10.030649
PRIMER_LEFT_35_PENALTY=5.781271
PRIMER_RIGHT_35_PENALTY=4.249378
PRIMER_LEFT_35_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_35_SEQUENCE=GCTTAGGAGATTTCTCAACCTCAA
PRIMER_LEFT_35=919,22
PRIMER_RIGHT_35=1844,24
PRIMER_LEFT_35_TM=56.219
PRIMER_RIGHT_35_TM=60.249
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=41.667
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.6000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=926
PRIMER_PAIR_36_PENALTY=10.058959
PRIMER_LEFT_36_PENALTY=5.781271
PRIMER_RIGHT_36_PENALTY=4.277689
PRIMER_LEFT_36_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_36_SEQUENCE=CAACTCCAGCGACCTCACAAG
PRIMER_LEFT_36=919,22
PRIMER_RIGHT_36=1757,21
PRIMER_LEFT_36_TM=56.219
PRIMER_RIGHT_36_TM=63.278
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=57.143
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.6000
PRIMER_RIGHT_36_END_STABILITY=6.7000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=839
PRIMER_PAIR_37_PENALTY=10.126574
PRIMER_LEFT_37_PENALTY=5.781271
PRIMER_RIGHT_37_PENALTY=4.345304
PRIMER_LEFT_37_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_37_SEQUENCE=AAACCTCGGGATATCAACTC
PRIMER_LEFT_37=919,22
PRIMER_RIGHT_37=1771,20
PRIMER_LEFT_37_TM=56.219
PRIMER_RIGHT_37_TM=55.655
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.6000
PRIMER_RIGHT_37_END_STABILITY=6.4000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=853
PRIMER_PAIR_38_PENALTY=10.349735
PRIMER_LEFT_38_PENALTY=5.781271
PRIMER_RIGHT_38_PENALTY=4.568464
PRIMER_LEFT_38_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_38_SEQUENCE=TTCTCAACCTCAACAAGACACATC
PRIMER_LEFT_38=919,22
PRIMER_RIGHT_38=1833,24
PRIMER_LEFT_38_TM=56.219
PRIMER_RIGHT_38_TM=60.568
PRIMER_LEFT_38_GC_PERCENT=40.909
PRIMER_RIGHT_38_GC_PERCENT=41.667
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.6000
PRIMER_RIGHT_38_END_STABILITY=6.3000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=915
PRIMER_PAIR_39_PENALTY=10.491650
PRIMER_LEFT_39_PENALTY=5.781271
PRIMER_RIGHT_39_PENALTY=4.710379
PRIMER_LEFT_39_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_39_SEQUENCE=CTCAACCTCAACAAGACACATC
PRIMER_LEFT_39=919,22
PRIMER_RIGHT_39=1831,22
PRIMER_LEFT_39_TM=56.219
PRIMER_RIGHT_39_TM=57.290
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=45.455
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.6000
PRIMER_RIGHT_39_END_STABILITY=6.3000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=913
PRIMER_PAIR_40_PENALTY=10.529442
PRIMER_LEFT_40_PENALTY=5.781271
PRIMER_RIGHT_40_PENALTY=4.748171
PRIMER_LEFT_40_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_40_SEQUENCE=AAAACCTCGGGATATCAACT
PRIMER_LEFT_40=919,22
PRIMER_RIGHT_40=1772,20
PRIMER_LEFT_40_TM=56.219
PRIMER_RIGHT_40_TM=55.252
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=9.6000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=854
PRIMER_PAIR_41_PENALTY=10.588415
PRIMER_LEFT_41_PENALTY=5.781271
PRIMER_RIGHT_41_PENALTY=4.807144
PRIMER_LEFT_41_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_41_SEQUENCE=GATTTCTCAACCTCAACAAGACAC
PRIMER_LEFT_41=919,22
PRIMER_RIGHT_41=1836,24
PRIMER_LEFT_41_TM=56.219
PRIMER_RIGHT_41_TM=59.193
PRIMER_LEFT_41_GC_PERCENT=40.909
PRIMER_RIGHT_41_GC_PERCENT=41.667
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.6000
PRIMER_RIGHT_41_END_STABILITY=6.1000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=918
PRIMER_PAIR_42_PENALTY=10.612312
PRIMER_LEFT_42_PENALTY=5.781271
PRIMER_RIGHT_42_PENALTY=4.831041
PRIMER_LEFT_42_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_42_SEQUENCE=AACCTCAACAAGACACATCCT
PRIMER_LEFT_42=919,22
PRIMER_RIGHT_42=1828,21
PRIMER_LEFT_42_TM=56.219
PRIMER_RIGHT_42_TM=56.169
PRIMER_LEFT_42_GC_PERCENT=40.909
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.6000
PRIMER_RIGHT_42_END_STABILITY=7.8000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=910
PRIMER_PAIR_43_PENALTY=10.689458
PRIMER_LEFT_43_PENALTY=5.781271
PRIMER_RIGHT_43_PENALTY=4.908187
PRIMER_LEFT_43_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_43_SEQUENCE=TCAACCTCAACAAGACACATC
PRIMER_LEFT_43=919,22
PRIMER_RIGHT_43=1830,21
PRIMER_LEFT_43_TM=56.219
PRIMER_RIGHT_43_TM=56.092
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.6000
PRIMER_RIGHT_43_END_STABILITY=6.3000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=912
PRIMER_PAIR_44_PENALTY=10.754190
PRIMER_LEFT_44_PENALTY=5.781271
PRIMER_RIGHT_44_PENALTY=4.972919
PRIMER_LEFT_44_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_44_SEQUENCE=GGAGATTTCTCAACCTCAACAAGA
PRIMER_LEFT_44=919,22
PRIMER_RIGHT_44=1839,24
PRIMER_LEFT_44_TM=56.219
PRIMER_RIGHT_44_TM=60.973
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.6000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=921
PRIMER_PAIR_45_PENALTY=10.815326
PRIMER_LEFT_45_PENALTY=5.781271
PRIMER_RIGHT_45_PENALTY=5.034055
PRIMER_LEFT_45_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_45_SEQUENCE=CGGGATATCAACTCCAGCGAC
PRIMER_LEFT_45=919,22
PRIMER_RIGHT_45=1765,21
PRIMER_LEFT_45_TM=56.219
PRIMER_RIGHT_45_TM=64.034
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=57.143
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=9.6000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=847
PRIMER_PAIR_46_PENALTY=10.823184
PRIMER_LEFT_46_PENALTY=5.781271
PRIMER_RIGHT_46_PENALTY=5.041913
PRIMER_LEFT_46_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_46_SEQUENCE=TTTCTCAACCTCAACAAGACAC
PRIMER_LEFT_46=919,22
PRIMER_RIGHT_46=1834,22
PRIMER_LEFT_46_TM=56.219
PRIMER_RIGHT_46_TM=56.958
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=40.909
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=6.1000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=916
PRIMER_PAIR_47_PENALTY=10.828543
PRIMER_LEFT_47_PENALTY=5.781271
PRIMER_RIGHT_47_PENALTY=5.047272
PRIMER_LEFT_47_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_47_SEQUENCE=TATCAACTCCAGCGACCTCACA
PRIMER_LEFT_47=919,22
PRIMER_RIGHT_47=1760,22
PRIMER_LEFT_47_TM=56.219
PRIMER_RIGHT_47_TM=63.047
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=2.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=842
PRIMER_PAIR_48_PENALTY=10.870008
PRIMER_LEFT_48_PENALTY=5.781271
PRIMER_RIGHT_48_PENALTY=5.088737
PRIMER_LEFT_48_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_48_SEQUENCE=AGATTTCTCAACCTCAACAAGACAC
PRIMER_LEFT_48=919,22
PRIMER_RIGHT_48=1837,25
PRIMER_LEFT_48_TM=56.219
PRIMER_RIGHT_48_TM=60.089
PRIMER_LEFT_48_GC_PERCENT=40.909
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=6.1000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=919
PRIMER_PAIR_49_PENALTY=10.946641
PRIMER_LEFT_49_PENALTY=5.781271
PRIMER_RIGHT_49_PENALTY=5.165371
PRIMER_LEFT_49_SEQUENCE=CTTACACGTAAACCTTAACCGA
PRIMER_RIGHT_49_SEQUENCE=TAGGAGATTTCTCAACCTCAACAAG
PRIMER_LEFT_49=919,22
PRIMER_RIGHT_49=1841,25
PRIMER_LEFT_49_TM=56.219
PRIMER_RIGHT_49_TM=60.165
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.6000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=923
=
SEQUENCE_ID=MAGIC.329_1_15680923_15681437_zu-0_ws-0_13174_11269_TRUE
SEQUENCE_TARGET=950,614
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_16485339_16485354_bur-0_can-0_311_28303_TRUE
SEQUENCE_TARGET=950,115
SEQUENCE_TEMPLATE=AAAAAAAAAATCAATGGAAGGAGGAGGNAGAGNACCAAATCAAACGATTCTCAGTGAAATAGAACATATGCCTGAAGCTCCACGTCAACGTATCTCTCATCACCGTCGAGCTCGCTCTGAAACCTTCTTCTCCGGCGAATCAATCGACGATCTCCTCTTATTCGATCCTTCCGANATCGATTTCTCTTCTCTAGACTTCCTCAACGCTCCACCACCACCACNACAATCACAACAACAACCNCAAGCTTCTCCCATGTCCGTTGATTCNGAAGAAACCTCATCNAACGGTGTTGTTCCTCCNAANNCTCTTCCTCCNAAACCCGAAGCTAGATTCGGTCGCCATGTTCGTAGCTTCTCGGTTGATTNCGATTTCTTCGATGATTTGGGTGTTACTGAGGAGAAGTNTNTAGCTACAAGTTCAGGAGAGAAGAAGAAAGGGNATCATCATCATAGCAGGAGTAATTCTATGGATGGAGAGATGAGTTCGGCGTCGTTTAATATCGAATCGATTTTAGCTTCTGTGAGTGGTAAAGATAGTGGGAAGAAGAATATGGGTATGGGTGGTGATAGACTTGCTGAGCTTGCTTTGCTTGATCCTAAAAGAGCTAAAAGGTATNTAATTTAGTGTTGNNNTTTACTTAAANNTTGTAACTTNTTTGNGATTTGATTGAGGTTTGAANNAGTGTTTTNNGNTGNNNTTTTGATAGGATTTTAGCGAATAGACAATCTGCGGCGAGGTCGAAAGAGAGGAAGATTAGGTATACTGGTGAGTTAGAGAGGAAGGTTCAGACACTTCAGAATGAAGCTACTACATTGTCTGCTCAAGTNACTATGTTACAGGTAATGTTTTNTATTACTTNGTCATNGTGTTTATGCAATTGGTGTGACTAGTGAAGGTTGGTGAGTANATTACNNNNNNTCTNNNNANCTNTNTNNNNNNNNNNNCTGATNTNNTNANTNNAGNTTNNNNNNNNGNACTNNNNNNNNTNNCTTGATTNGNNCNTGGCAGCTTNTANGCTTGTANTGNTGCTTAGTNTGTTTAAGATTGCTTCTTNGTGTNTAGAGAGGACATGTGTTTGCTACTAGNNNNNNNNATAGATGCAGNNTTATAAGATATNNTATTNTNATGTTAATTTAGCNNATNAATCAACATGTGAGATATGTCANTAGTGTAATGTATACANTTGCAAGAGTGTAANNTTTGTTTNCACTTTAGTGATAAAAGCAACATAGGAGTAAGATNAAATGATTCATGTNTCTTGCATCTCTAATCTGTGCTTTTTCTTATATTGTGTAGAGAGGAACATCAGAGCTGAACACTGAAAATAAACACCTCAAAATGCNGCTTCAAGCTTTAGAGCAACAAGCTGAACTTAGGGATGGTNAAGCCATTTTCTTNTTCATTGGNTCGTTCTCAATATGTANACCNAAGTCNNCNTTACGATATNTATTGTTCGGTGTAATTTTCAGCTTTGAATGAAGCNCTGCGGGATGAACTNAACCGACTTAAGGTGGTAGCTGGAGAAATTCCTCAGGGGAANGGAAATTCNTACAACCGTGCTCAATTCTCATCTCAGCAATCGGCAATGAATCAGTTTGGGAACAANANGAACCANCAGATGAGTACAAACGGGCAGCCATCGCTCCCAAGCTACATGGATTTCACCAAGAGAGGCTGAGTTCGTGTCACCTATATATGTTTGCTGAGTTATAAATACGTTATATTCATTTCGGGCTGCAATATTTGATGTATGTAAAAAGTATGTATGTATCCATGTATNATGGCTTTTGNNNTGTTANCGTCTATGCAGATATGTTCATGGCGANATCGGTCTATNTTTACNAGTAGTATCCATTGTAATATACTANCCAAAAATCTTTATAGTNAGCACTTTTCCTAGGCATTAAGCACCGATCATCAAACACGCAGCTAAATCNGNNGCTTAAGGCANNTACTTGCTGTTACGATAATGCTAAAACTNGGTAAACNATTGCGAANGACNAAGTGAAGGTTG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.508608
PRIMER_LEFT_0_PENALTY=0.841762
PRIMER_RIGHT_0_PENALTY=0.666846
PRIMER_LEFT_0_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_0_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_0=733,20
PRIMER_RIGHT_0=1637,20
PRIMER_LEFT_0_TM=59.158
PRIMER_RIGHT_0_TM=60.667
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=905
PRIMER_PAIR_1_PENALTY=1.684734
PRIMER_LEFT_1_PENALTY=0.841762
PRIMER_RIGHT_1_PENALTY=0.842972
PRIMER_LEFT_1_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_1_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_1=733,20
PRIMER_RIGHT_1=1601,20
PRIMER_LEFT_1_TM=59.158
PRIMER_RIGHT_1_TM=60.843
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=869
PRIMER_PAIR_2_PENALTY=1.714406
PRIMER_LEFT_2_PENALTY=0.841762
PRIMER_RIGHT_2_PENALTY=0.872644
PRIMER_LEFT_2_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_2_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_2=733,20
PRIMER_RIGHT_2=1690,20
PRIMER_LEFT_2_TM=59.158
PRIMER_RIGHT_2_TM=60.873
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=958
PRIMER_PAIR_3_PENALTY=1.823411
PRIMER_LEFT_3_PENALTY=0.777580
PRIMER_RIGHT_3_PENALTY=1.045831
PRIMER_LEFT_3_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_3_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_3=578,20
PRIMER_RIGHT_3=1530,21
PRIMER_LEFT_3_TM=59.222
PRIMER_RIGHT_3_TM=59.954
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=953
PRIMER_PAIR_4_PENALTY=1.887594
PRIMER_LEFT_4_PENALTY=0.841762
PRIMER_RIGHT_4_PENALTY=1.045831
PRIMER_LEFT_4_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_4_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_4=733,20
PRIMER_RIGHT_4=1530,21
PRIMER_LEFT_4_TM=59.158
PRIMER_RIGHT_4_TM=59.954
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=798
PRIMER_PAIR_5_PENALTY=1.960235
PRIMER_LEFT_5_PENALTY=0.841762
PRIMER_RIGHT_5_PENALTY=1.118473
PRIMER_LEFT_5_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_5_SEQUENCE=TAGGTGACACGAACTCAGCC
PRIMER_LEFT_5=733,20
PRIMER_RIGHT_5=1692,20
PRIMER_LEFT_5_TM=59.158
PRIMER_RIGHT_5_TM=58.882
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=960
PRIMER_PAIR_6_PENALTY=1.982603
PRIMER_LEFT_6_PENALTY=0.841762
PRIMER_RIGHT_6_PENALTY=1.140841
PRIMER_LEFT_6_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_6_SEQUENCE=CTGATTCATTGCCGATTGCT
PRIMER_LEFT_6=733,20
PRIMER_RIGHT_6=1594,20
PRIMER_LEFT_6_TM=59.158
PRIMER_RIGHT_6_TM=61.141
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=862
PRIMER_PAIR_7_PENALTY=2.053590
PRIMER_LEFT_7_PENALTY=0.777580
PRIMER_RIGHT_7_PENALTY=1.276010
PRIMER_LEFT_7_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_7_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_7=578,20
PRIMER_RIGHT_7=1571,20
PRIMER_LEFT_7_TM=59.222
PRIMER_RIGHT_7_TM=58.724
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.8000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=994
PRIMER_PAIR_8_PENALTY=2.117772
PRIMER_LEFT_8_PENALTY=0.841762
PRIMER_RIGHT_8_PENALTY=1.276010
PRIMER_LEFT_8_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_8_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_8=733,20
PRIMER_RIGHT_8=1571,20
PRIMER_LEFT_8_TM=59.158
PRIMER_RIGHT_8_TM=58.724
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=839
PRIMER_PAIR_9_PENALTY=2.181722
PRIMER_LEFT_9_PENALTY=0.095771
PRIMER_RIGHT_9_PENALTY=2.085951
PRIMER_LEFT_9_SEQUENCE=CTAGATTCGGTCGCCATGTT
PRIMER_RIGHT_9_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_9=326,20
PRIMER_RIGHT_9=1321,22
PRIMER_LEFT_9_TM=60.096
PRIMER_RIGHT_9_TM=59.914
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=996
PRIMER_PAIR_10_PENALTY=2.340266
PRIMER_LEFT_10_PENALTY=1.938224
PRIMER_RIGHT_10_PENALTY=0.402042
PRIMER_LEFT_10_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_10_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_10=732,20
PRIMER_RIGHT_10=1683,20
PRIMER_LEFT_10_TM=61.938
PRIMER_RIGHT_10_TM=59.598
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=8.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=952
PRIMER_PAIR_11_PENALTY=2.394411
PRIMER_LEFT_11_PENALTY=1.348580
PRIMER_RIGHT_11_PENALTY=1.045831
PRIMER_LEFT_11_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_11_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_11=572,20
PRIMER_RIGHT_11=1530,21
PRIMER_LEFT_11_TM=61.349
PRIMER_RIGHT_11_TM=59.954
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=52.381
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=959
PRIMER_PAIR_12_PENALTY=2.423693
PRIMER_LEFT_12_PENALTY=1.377862
PRIMER_RIGHT_12_PENALTY=1.045831
PRIMER_LEFT_12_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_12_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_12=579,21
PRIMER_RIGHT_12=1530,21
PRIMER_LEFT_12_TM=59.622
PRIMER_RIGHT_12_TM=59.954
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.5000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=952
PRIMER_PAIR_13_PENALTY=2.429008
PRIMER_LEFT_13_PENALTY=0.777580
PRIMER_RIGHT_13_PENALTY=1.651428
PRIMER_LEFT_13_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_13_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_13=578,20
PRIMER_RIGHT_13=1572,21
PRIMER_LEFT_13_TM=59.222
PRIMER_RIGHT_13_TM=60.651
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.8000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=995
PRIMER_PAIR_14_PENALTY=2.454032
PRIMER_LEFT_14_PENALTY=0.841762
PRIMER_RIGHT_14_PENALTY=1.612269
PRIMER_LEFT_14_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_14_SEQUENCE=GAGATGAGAATTGAGCACGG
PRIMER_LEFT_14=733,20
PRIMER_RIGHT_14=1574,20
PRIMER_LEFT_14_TM=59.158
PRIMER_RIGHT_14_TM=58.388
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=842
PRIMER_PAIR_15_PENALTY=2.468002
PRIMER_LEFT_15_PENALTY=2.065960
PRIMER_RIGHT_15_PENALTY=0.402042
PRIMER_LEFT_15_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_15_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_15=879,22
PRIMER_RIGHT_15=1683,20
PRIMER_LEFT_15_TM=60.066
PRIMER_RIGHT_15_TM=59.598
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=805
PRIMER_PAIR_16_PENALTY=2.468002
PRIMER_LEFT_16_PENALTY=2.065960
PRIMER_RIGHT_16_PENALTY=0.402042
PRIMER_LEFT_16_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_16_SEQUENCE=ACGAACTCAGCCTCTCTTGG
PRIMER_LEFT_16=879,22
PRIMER_RIGHT_16=1684,20
PRIMER_LEFT_16_TM=60.066
PRIMER_RIGHT_16_TM=59.598
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=806
PRIMER_PAIR_17_PENALTY=2.474358
PRIMER_LEFT_17_PENALTY=0.388407
PRIMER_RIGHT_17_PENALTY=2.085951
PRIMER_LEFT_17_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_17_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_17=468,20
PRIMER_RIGHT_17=1321,22
PRIMER_LEFT_17_TM=59.612
PRIMER_RIGHT_17_TM=59.914
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=10.2000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=854
PRIMER_PAIR_18_PENALTY=2.489553
PRIMER_LEFT_18_PENALTY=2.065960
PRIMER_RIGHT_18_PENALTY=0.423592
PRIMER_LEFT_18_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_18_SEQUENCE=ATATTGCAGCCCGAAATGAA
PRIMER_LEFT_18=879,22
PRIMER_RIGHT_18=1743,20
PRIMER_LEFT_18_TM=60.066
PRIMER_RIGHT_18_TM=60.424
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=865
PRIMER_PAIR_19_PENALTY=2.489553
PRIMER_LEFT_19_PENALTY=2.065960
PRIMER_RIGHT_19_PENALTY=0.423592
PRIMER_LEFT_19_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_19_SEQUENCE=ATCAAATATTGCAGCCCGAA
PRIMER_LEFT_19=879,22
PRIMER_RIGHT_19=1748,20
PRIMER_LEFT_19_TM=60.066
PRIMER_RIGHT_19_TM=60.424
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=10.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=870
PRIMER_PAIR_20_PENALTY=2.493191
PRIMER_LEFT_20_PENALTY=0.841762
PRIMER_RIGHT_20_PENALTY=1.651428
PRIMER_LEFT_20_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_20_SEQUENCE=GATGAGAATTGAGCACGGTTG
PRIMER_LEFT_20=733,20
PRIMER_RIGHT_20=1572,21
PRIMER_LEFT_20_TM=59.158
PRIMER_RIGHT_20_TM=60.651
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=840
PRIMER_PAIR_21_PENALTY=2.544250
PRIMER_LEFT_21_PENALTY=0.841762
PRIMER_RIGHT_21_PENALTY=1.702488
PRIMER_LEFT_21_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_21_SEQUENCE=ATTGCCGATTGCTGAGATGA
PRIMER_LEFT_21=733,20
PRIMER_RIGHT_21=1587,20
PRIMER_LEFT_21_TM=59.158
PRIMER_RIGHT_21_TM=61.702
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=6.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=855
PRIMER_PAIR_22_PENALTY=2.571769
PRIMER_LEFT_22_PENALTY=0.841762
PRIMER_RIGHT_22_PENALTY=1.730006
PRIMER_LEFT_22_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_22_SEQUENCE=CATGTAGCTTGGGAGCGATG
PRIMER_LEFT_22=733,20
PRIMER_RIGHT_22=1657,20
PRIMER_LEFT_22_TM=59.158
PRIMER_RIGHT_22_TM=61.730
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=925
PRIMER_PAIR_23_PENALTY=2.605069
PRIMER_LEFT_23_PENALTY=1.938224
PRIMER_RIGHT_23_PENALTY=0.666846
PRIMER_LEFT_23_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_23_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_23=732,20
PRIMER_RIGHT_23=1637,20
PRIMER_LEFT_23_TM=61.938
PRIMER_RIGHT_23_TM=60.667
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=906
PRIMER_PAIR_24_PENALTY=2.615615
PRIMER_LEFT_24_PENALTY=0.841762
PRIMER_RIGHT_24_PENALTY=1.773852
PRIMER_LEFT_24_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_24_SEQUENCE=ATAGGTGACACGAACTCAGCC
PRIMER_LEFT_24=733,20
PRIMER_RIGHT_24=1693,21
PRIMER_LEFT_24_TM=59.158
PRIMER_RIGHT_24_TM=59.226
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=52.381
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=9.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=961
PRIMER_PAIR_25_PENALTY=2.624590
PRIMER_LEFT_25_PENALTY=1.348580
PRIMER_RIGHT_25_PENALTY=1.276010
PRIMER_LEFT_25_SEQUENCE=TTGCTGAGCTTGCTTTGCTT
PRIMER_RIGHT_25_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_25=572,20
PRIMER_RIGHT_25=1571,20
PRIMER_LEFT_25_TM=61.349
PRIMER_RIGHT_25_TM=58.724
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=1000
PRIMER_PAIR_26_PENALTY=2.653872
PRIMER_LEFT_26_PENALTY=1.377862
PRIMER_RIGHT_26_PENALTY=1.276010
PRIMER_LEFT_26_SEQUENCE=GCTTGCTTTGCTTGATCCTAA
PRIMER_RIGHT_26_SEQUENCE=ATGAGAATTGAGCACGGTTG
PRIMER_LEFT_26=579,21
PRIMER_RIGHT_26=1571,20
PRIMER_LEFT_26_TM=59.622
PRIMER_RIGHT_26_TM=58.724
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.5000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=993
PRIMER_PAIR_27_PENALTY=2.678904
PRIMER_LEFT_27_PENALTY=2.012058
PRIMER_RIGHT_27_PENALTY=0.666846
PRIMER_LEFT_27_SEQUENCE=CGAGGTCGAAAGAGAGGAAGA
PRIMER_RIGHT_27_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_27=733,21
PRIMER_RIGHT_27=1637,20
PRIMER_LEFT_27_TM=61.012
PRIMER_RIGHT_27_TM=60.667
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=905
PRIMER_PAIR_28_PENALTY=2.683451
PRIMER_LEFT_28_PENALTY=0.841762
PRIMER_RIGHT_28_PENALTY=1.841689
PRIMER_LEFT_28_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_28_SEQUENCE=CTCAGCCTCTCTTGGTGAAA
PRIMER_LEFT_28=733,20
PRIMER_RIGHT_28=1679,20
PRIMER_LEFT_28_TM=59.158
PRIMER_RIGHT_28_TM=58.158
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=8.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=947
PRIMER_PAIR_29_PENALTY=2.724911
PRIMER_LEFT_29_PENALTY=0.388407
PRIMER_RIGHT_29_PENALTY=2.336505
PRIMER_LEFT_29_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_29_SEQUENCE=CCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_29=468,20
PRIMER_RIGHT_29=1378,21
PRIMER_LEFT_29_TM=59.612
PRIMER_RIGHT_29_TM=58.663
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=10.2000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=911
PRIMER_PAIR_30_PENALTY=2.732806
PRIMER_LEFT_30_PENALTY=2.065960
PRIMER_RIGHT_30_PENALTY=0.666846
PRIMER_LEFT_30_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_30_SEQUENCE=GCTGCCCGTTTGTACTCATC
PRIMER_LEFT_30=879,22
PRIMER_RIGHT_30=1637,20
PRIMER_LEFT_30_TM=60.066
PRIMER_RIGHT_30_TM=60.667
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=759
PRIMER_PAIR_31_PENALTY=2.735536
PRIMER_LEFT_31_PENALTY=0.388407
PRIMER_RIGHT_31_PENALTY=2.347129
PRIMER_LEFT_31_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_31_SEQUENCE=CCATCCCTAAGTTCAGCTTGTT
PRIMER_LEFT_31=468,20
PRIMER_RIGHT_31=1382,22
PRIMER_LEFT_31_TM=59.612
PRIMER_RIGHT_31_TM=59.653
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=10.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=915
PRIMER_PAIR_32_PENALTY=2.765798
PRIMER_LEFT_32_PENALTY=0.841762
PRIMER_RIGHT_32_PENALTY=1.924036
PRIMER_LEFT_32_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_32_SEQUENCE=ACTCAGCCTCTCTTGGTGAAA
PRIMER_LEFT_32=733,20
PRIMER_RIGHT_32=1680,21
PRIMER_LEFT_32_TM=59.158
PRIMER_RIGHT_32_TM=59.076
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=47.619
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.3000
PRIMER_PAIR_32_COMPL_ANY=8.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=948
PRIMER_PAIR_33_PENALTY=2.781195
PRIMER_LEFT_33_PENALTY=1.938224
PRIMER_RIGHT_33_PENALTY=0.842972
PRIMER_LEFT_33_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_33_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_33=732,20
PRIMER_RIGHT_33=1601,20
PRIMER_LEFT_33_TM=61.938
PRIMER_RIGHT_33_TM=60.843
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=10.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=870
PRIMER_PAIR_34_PENALTY=2.788857
PRIMER_LEFT_34_PENALTY=0.841762
PRIMER_RIGHT_34_PENALTY=1.947095
PRIMER_LEFT_34_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_34_SEQUENCE=CCAAACTGATTCATTGCCGA
PRIMER_LEFT_34=733,20
PRIMER_RIGHT_34=1599,20
PRIMER_LEFT_34_TM=59.158
PRIMER_RIGHT_34_TM=61.947
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=11.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=867
PRIMER_PAIR_35_PENALTY=2.810867
PRIMER_LEFT_35_PENALTY=1.938224
PRIMER_RIGHT_35_PENALTY=0.872644
PRIMER_LEFT_35_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_35_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_35=732,20
PRIMER_RIGHT_35=1690,20
PRIMER_LEFT_35_TM=61.938
PRIMER_RIGHT_35_TM=60.873
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=60.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=9.4000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=959
PRIMER_PAIR_36_PENALTY=2.812798
PRIMER_LEFT_36_PENALTY=0.388407
PRIMER_RIGHT_36_PENALTY=2.424392
PRIMER_LEFT_36_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_36_SEQUENCE=CCCTAAGTTCAGCTTGTTGCTC
PRIMER_LEFT_36=468,20
PRIMER_RIGHT_36=1378,22
PRIMER_LEFT_36_TM=59.612
PRIMER_RIGHT_36_TM=60.424
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.2000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=911
PRIMER_PAIR_37_PENALTY=2.812798
PRIMER_LEFT_37_PENALTY=0.388407
PRIMER_RIGHT_37_PENALTY=2.424392
PRIMER_LEFT_37_SEQUENCE=GGATGGAGAGATGAGTTCGG
PRIMER_RIGHT_37_SEQUENCE=TCCCTAAGTTCAGCTTGTTGCT
PRIMER_LEFT_37=468,20
PRIMER_RIGHT_37=1379,22
PRIMER_LEFT_37_TM=59.612
PRIMER_RIGHT_37_TM=60.424
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=45.455
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=5.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.2000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=912
PRIMER_PAIR_38_PENALTY=2.855030
PRIMER_LEFT_38_PENALTY=2.012058
PRIMER_RIGHT_38_PENALTY=0.842972
PRIMER_LEFT_38_SEQUENCE=CGAGGTCGAAAGAGAGGAAGA
PRIMER_RIGHT_38_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_38=733,21
PRIMER_RIGHT_38=1601,20
PRIMER_LEFT_38_TM=61.012
PRIMER_RIGHT_38_TM=60.843
PRIMER_LEFT_38_GC_PERCENT=52.381
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.7000
PRIMER_RIGHT_38_END_STABILITY=10.0000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=869
PRIMER_PAIR_39_PENALTY=2.863531
PRIMER_LEFT_39_PENALTY=0.777580
PRIMER_RIGHT_39_PENALTY=2.085951
PRIMER_LEFT_39_SEQUENCE=AGCTTGCTTTGCTTGATCCT
PRIMER_RIGHT_39_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_39=578,20
PRIMER_RIGHT_39=1321,22
PRIMER_LEFT_39_TM=59.222
PRIMER_RIGHT_39_TM=59.914
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.8000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=744
PRIMER_PAIR_40_PENALTY=2.872108
PRIMER_LEFT_40_PENALTY=0.786156
PRIMER_RIGHT_40_PENALTY=2.085951
PRIMER_LEFT_40_SEQUENCE=CCATGTTCGTAGCTTCTCGG
PRIMER_RIGHT_40_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_40=339,20
PRIMER_RIGHT_40=1321,22
PRIMER_LEFT_40_TM=60.786
PRIMER_RIGHT_40_TM=59.914
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.9000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=983
PRIMER_PAIR_41_PENALTY=2.908932
PRIMER_LEFT_41_PENALTY=2.065960
PRIMER_RIGHT_41_PENALTY=0.842972
PRIMER_LEFT_41_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_41_SEQUENCE=TCCCAAACTGATTCATTGCC
PRIMER_LEFT_41=879,22
PRIMER_RIGHT_41=1601,20
PRIMER_LEFT_41_TM=60.066
PRIMER_RIGHT_41_TM=60.843
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=10.0000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=723
PRIMER_PAIR_42_PENALTY=2.919343
PRIMER_LEFT_42_PENALTY=0.841762
PRIMER_RIGHT_42_PENALTY=2.077581
PRIMER_LEFT_42_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_42_SEQUENCE=GATTCATTGCCGATTGCTGA
PRIMER_LEFT_42=733,20
PRIMER_RIGHT_42=1592,20
PRIMER_LEFT_42_TM=59.158
PRIMER_RIGHT_42_TM=62.078
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=860
PRIMER_PAIR_43_PENALTY=2.927714
PRIMER_LEFT_43_PENALTY=0.841762
PRIMER_RIGHT_43_PENALTY=2.085951
PRIMER_LEFT_43_SEQUENCE=CGAGGTCGAAAGAGAGGAAG
PRIMER_RIGHT_43_SEQUENCE=CAGTGTTCAGCTCTGATGTTCC
PRIMER_LEFT_43=733,20
PRIMER_RIGHT_43=1321,22
PRIMER_LEFT_43_TM=59.158
PRIMER_RIGHT_43_TM=59.914
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=589
PRIMER_PAIR_44_PENALTY=2.937401
PRIMER_LEFT_44_PENALTY=2.065960
PRIMER_RIGHT_44_PENALTY=0.871441
PRIMER_LEFT_44_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_44_SEQUENCE=TGACACGAACTCAGCCTCTC
PRIMER_LEFT_44=879,22
PRIMER_RIGHT_44=1688,20
PRIMER_LEFT_44_TM=60.066
PRIMER_RIGHT_44_TM=59.129
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=810
PRIMER_PAIR_45_PENALTY=2.938604
PRIMER_LEFT_45_PENALTY=2.065960
PRIMER_RIGHT_45_PENALTY=0.872644
PRIMER_LEFT_45_SEQUENCE=TGGTGTGACTAGTGAAGGTTGG
PRIMER_RIGHT_45_SEQUENCE=GGTGACACGAACTCAGCCTC
PRIMER_LEFT_45=879,22
PRIMER_RIGHT_45=1690,20
PRIMER_LEFT_45_TM=60.066
PRIMER_RIGHT_45_TM=60.873
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=60.000
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=812
PRIMER_PAIR_46_PENALTY=2.947204
PRIMER_LEFT_46_PENALTY=1.901373
PRIMER_RIGHT_46_PENALTY=1.045831
PRIMER_LEFT_46_SEQUENCE=GCTGAGCTTGCTTTGCTTGA
PRIMER_RIGHT_46_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_46=574,20
PRIMER_RIGHT_46=1530,21
PRIMER_LEFT_46_TM=61.901
PRIMER_RIGHT_46_TM=59.954
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=9.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=957
PRIMER_PAIR_47_PENALTY=2.976022
PRIMER_LEFT_47_PENALTY=2.573980
PRIMER_RIGHT_47_PENALTY=0.402042
PRIMER_LEFT_47_SEQUENCE=ATAGACAATCTGCGGCGAGG
PRIMER_RIGHT_47_SEQUENCE=CGAACTCAGCCTCTCTTGGT
PRIMER_LEFT_47=718,20
PRIMER_RIGHT_47=1683,20
PRIMER_LEFT_47_TM=62.574
PRIMER_RIGHT_47_TM=59.598
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=966
PRIMER_PAIR_48_PENALTY=2.976022
PRIMER_LEFT_48_PENALTY=2.573980
PRIMER_RIGHT_48_PENALTY=0.402042
PRIMER_LEFT_48_SEQUENCE=ATAGACAATCTGCGGCGAGG
PRIMER_RIGHT_48_SEQUENCE=ACGAACTCAGCCTCTCTTGG
PRIMER_LEFT_48=718,20
PRIMER_RIGHT_48=1684,20
PRIMER_LEFT_48_TM=62.574
PRIMER_RIGHT_48_TM=59.598
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=55.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.9000
PRIMER_RIGHT_48_END_STABILITY=8.5000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=967
PRIMER_PAIR_49_PENALTY=2.984055
PRIMER_LEFT_49_PENALTY=1.938224
PRIMER_RIGHT_49_PENALTY=1.045831
PRIMER_LEFT_49_SEQUENCE=GCGAGGTCGAAAGAGAGGAA
PRIMER_RIGHT_49_SEQUENCE=GGAATTTCTCCAGCTACCACC
PRIMER_LEFT_49=732,20
PRIMER_RIGHT_49=1530,21
PRIMER_LEFT_49_TM=61.938
PRIMER_RIGHT_49_TM=59.954
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=9.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=799
=
SEQUENCE_ID=MAGIC.105_3_9625866_9625889_oy-0_kn-0_12524_268977_TRUE
SEQUENCE_TARGET=950,123
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNAATATCANGCGNTTAANTTTTGTTTNTCNCNATTNNNTTTNTTATNATAATNNNNNNNNNNNNGTATNTAGGTGATATGANAAAAANATAAAAAANGTNAATGANAANAAAGNTGNTTTTAAANTATANTANNNGTTGATTAANAGTGGTTTTACNCANAAATAGAGAANTGAGAGATTATTCGAAGTTTTAANANGTTTGAANAAAATTGTTTGTACATGTTGAAAAANCANANCANGAATTGTTANGATNANAATTGATNGTTCGAACCAAAANTAATGTTANNAAACCATATATGAAACANGGTTTNCCATNATTCATTTATCNNNNNNNNGAANTAGTNNNNNNNTATTTTTCAAACTTCATAAGAAGTNAGTTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCANNTGTTCTTNAATGATACNACNTTGANGTTNTTTTCATNCTGCTTTNTTNAATTCAACTTTGAANGNACATAGTTATCAATTNGAATTAACNTTAAAANGANNTGAATCTATATTTCTTCTCANNNTAGTCTTATGGATAGCANCTTCTTACATGTCTCTNGTAAAAGNTTNTTTTGTCGTCTCTGTCTNGATTTGTTTGTTGTGGTNGGGGTTTGCCNATCCACTNTTAGTTTATCCGCNACNAAAAACATCCGTACAAGATAAAAACTTTGAATTGNAAATATTNANATTNGATAATTTCAAATANNNNNNNTCAAAAGTTCNTACT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_22940614_22940769_oy-0_sf-2_38792_382598_TRUE
SEQUENCE_TARGET=950,255
SEQUENCE_TEMPLATE=TTTGAAGTNTGACTTANGCCCGGCCCACTAGAGATATTGATAGTTACTTGCGTTTTAGGATTTGCTAGGGTTNTTNAGGGGTTTGTCTTTGTTGCNCTTTTTGAAAGCTATGGCTGAAATCAGCGGGNTGCCTGATGACTTGCTGGTGAAGATACTAGCGTTTCTTCCAACAAAAGTTGCTATATCAACTAGCGTTTTGTCAAAACAATGGCGGTTTCTTTGGANGTGGTTGCCTAAACTTAAGTACGATGATTACGATGACATTACTGATGGTTTCAATTCCGTGTCTGCATTCCAGACATATCGGGATTTTATTGCCAAGAATTTGCCATTACANAGAGCTCCCATCATAGAAAGTTTGAGCCTCGGATTTCGTTGTGGAACACTTCAACCTGAGGATTTGAAATCGTGGGTTGAAGTTGCAGTTTCTCGTAGTGTGCGTGAGCTAAGTATCCTTGCATATTATAGGAATNATTATGCATTATCGTCGTCTAGTTTGTATACCTGNAAATCGCTCGTGACTTTGNAAGGCTTTAACATTCGTGTGGATGTTCCTCCAACAGTTTGTCTCCTGCCTTCCTTGAGAACTTTGGAACTTAAACGTGTGAGATACTTAAACGAAGATTCTCTTCGAATGCTCCTATCCTTTTGCCCTGTTCTGGAATATCNAAGTATAGANNGACACGATAATGACAATTTGAGAGGGTTAGTTGTTGATGTNCCGTCCTTGCNGCGTTTATCTTTAACTTCATATACTGGATGTTCTTCTGACGATTATGTAATAGTTACACCTTCTTTGAAGTATTTCAAAGCTTTTGATTACAGAAGTGAAATTTCCTCCTATAAGATTGAGAAAATTCCAGAGCTTGAAGAGGCAGATATCAGTATTGAGCGAAATCCCGAGAAGCTTTTCGTATATTTCAAATCTNTCAAANGTCTTTCATTACAAGTAGACTTCAACAGCAAGGAAGAGGTAATCTTTTTNCATCTATNAACTNNCNAGTTTTCAANTTTGCATTATATTTACTTNTAACACTAAATTTGTTNCNTTTTCACGTGTATAGCCCGGGTATGATTCTGGTATTGTCTTCAATCNTCTTGAAGANCTGACGCNATANATANACGACGCTAATTGGTNCAAGTTACTTNTCCGGTNGCTCAANGATTCACCTAAACTCCGAGTCCTCGANATCAGCAACTCCAAGTCATTTGTAAGTTTTTNTTTGCNAGANCTGATATATGGTGTNATCTCAACTCTNNTNTTTCANTTTTTTNANTTNAATTTCAGTATAAAGAAAAAATTGGGGAGTATCTACCGGTTAGCTGGAGCAAAAATCAGGGATCCGTTCCTAAATGTTTCTTGAATAGTCTNGAAACTTTCAGGGTTAAATGGTACTACAGCGAGGAGCAAGAAGATAGGGNTTTNTTGAGTCTTATCTTCAAACATGCTCGATGTCTGAAATCTACATCAATCCTGCACAGATGATGTTATGTTCTCCACAACTGAAACTTGAAAATGATCTATGAGNCGTCGTATTTNTTTTGTTATCCAAGAACACTTNGGTTTTTATTTGAATGTGGTACTTGTTTAAGAACTGAACTNNNNTGTTTTAGATCATTCGTCTCCTCCATATAATAGACGACATCTTGTATCATTTAATACTCAGATTCAGCTTGATNATCCCAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAATAAAATAGGGTTTTTANTNCCCATAGCNTTCNCTGCAAACTTCCTGATCTTAGCTCCTTNAAAGGCATTTGGTGCATTCTTCTTAAANGTGCCATTACAATTTACAAGTTCATTANGNNNNNNNNNNNNNNNNNNNNNNNNCATAAGTAGGTTCNTAATCTCATNGAACCAAACCCACCTGAAAATACATTCCAAAACTGAACCTAATCTTANTTTGTATTTGGAGTTTACANAAGTACACTAATTNAGAAGTTTGCTGTGTATGGATTTTACAAAAAAATCTCAACTACAATAAGGACCTTAAAATATACNCATGCACTCAAAAAGAGTTTGACATGACGACTGGCCCAGTAGCAGGCCCCTCAGATATTTATGTTTATTAAACCATTNGATGGGCCTATAANTGGAAGCCNAACGGTCTGATGAGTGGTGGT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.169668
PRIMER_LEFT_0_PENALTY=0.156061
PRIMER_RIGHT_0_PENALTY=0.013607
PRIMER_LEFT_0_SEQUENCE=CAGTTTGTCTCCTGCCTTCC
PRIMER_RIGHT_0_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_0=560,20
PRIMER_RIGHT_0=1343,20
PRIMER_LEFT_0_TM=59.844
PRIMER_RIGHT_0_TM=60.014
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=784
PRIMER_PAIR_1_PENALTY=0.287212
PRIMER_LEFT_1_PENALTY=0.156061
PRIMER_RIGHT_1_PENALTY=0.131151
PRIMER_LEFT_1_SEQUENCE=CAGTTTGTCTCCTGCCTTCC
PRIMER_RIGHT_1_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_1=560,20
PRIMER_RIGHT_1=1349,20
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=790
PRIMER_PAIR_2_PENALTY=0.380058
PRIMER_LEFT_2_PENALTY=0.366450
PRIMER_RIGHT_2_PENALTY=0.013607
PRIMER_LEFT_2_SEQUENCE=TTTGTCTCCTGCCTTCCTTG
PRIMER_RIGHT_2_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_2=563,20
PRIMER_RIGHT_2=1343,20
PRIMER_LEFT_2_TM=60.366
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=781
PRIMER_PAIR_3_PENALTY=0.389605
PRIMER_LEFT_3_PENALTY=0.156061
PRIMER_RIGHT_3_PENALTY=0.233544
PRIMER_LEFT_3_SEQUENCE=CAGTTTGTCTCCTGCCTTCC
PRIMER_RIGHT_3_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_3=560,20
PRIMER_RIGHT_3=1350,20
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=59.766
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=791
PRIMER_PAIR_4_PENALTY=0.432712
PRIMER_LEFT_4_PENALTY=0.419105
PRIMER_RIGHT_4_PENALTY=0.013607
PRIMER_LEFT_4_SEQUENCE=ATGCTCCTATCCTTTTGCCC
PRIMER_RIGHT_4_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_4=634,20
PRIMER_RIGHT_4=1343,20
PRIMER_LEFT_4_TM=60.419
PRIMER_RIGHT_4_TM=60.014
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=710
PRIMER_PAIR_5_PENALTY=0.483608
PRIMER_LEFT_5_PENALTY=0.470001
PRIMER_RIGHT_5_PENALTY=0.013607
PRIMER_LEFT_5_SEQUENCE=GTCTCCTGCCTTCCTTGAGA
PRIMER_RIGHT_5_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_5=566,20
PRIMER_RIGHT_5=1343,20
PRIMER_LEFT_5_TM=59.530
PRIMER_RIGHT_5_TM=60.014
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=778
PRIMER_PAIR_6_PENALTY=0.497602
PRIMER_LEFT_6_PENALTY=0.366450
PRIMER_RIGHT_6_PENALTY=0.131151
PRIMER_LEFT_6_SEQUENCE=TTTGTCTCCTGCCTTCCTTG
PRIMER_RIGHT_6_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_6=563,20
PRIMER_RIGHT_6=1349,20
PRIMER_LEFT_6_TM=60.366
PRIMER_RIGHT_6_TM=60.131
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=787
PRIMER_PAIR_7_PENALTY=0.550256
PRIMER_LEFT_7_PENALTY=0.419105
PRIMER_RIGHT_7_PENALTY=0.131151
PRIMER_LEFT_7_SEQUENCE=ATGCTCCTATCCTTTTGCCC
PRIMER_RIGHT_7_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_7=634,20
PRIMER_RIGHT_7=1349,20
PRIMER_LEFT_7_TM=60.419
PRIMER_RIGHT_7_TM=60.131
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=11.2000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=716
PRIMER_PAIR_8_PENALTY=0.599995
PRIMER_LEFT_8_PENALTY=0.366450
PRIMER_RIGHT_8_PENALTY=0.233544
PRIMER_LEFT_8_SEQUENCE=TTTGTCTCCTGCCTTCCTTG
PRIMER_RIGHT_8_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_8=563,20
PRIMER_RIGHT_8=1350,20
PRIMER_LEFT_8_TM=60.366
PRIMER_RIGHT_8_TM=59.766
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=788
PRIMER_PAIR_9_PENALTY=0.601152
PRIMER_LEFT_9_PENALTY=0.470001
PRIMER_RIGHT_9_PENALTY=0.131151
PRIMER_LEFT_9_SEQUENCE=GTCTCCTGCCTTCCTTGAGA
PRIMER_RIGHT_9_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_9=566,20
PRIMER_RIGHT_9=1349,20
PRIMER_LEFT_9_TM=59.530
PRIMER_RIGHT_9_TM=60.131
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=784
PRIMER_PAIR_10_PENALTY=0.652649
PRIMER_LEFT_10_PENALTY=0.419105
PRIMER_RIGHT_10_PENALTY=0.233544
PRIMER_LEFT_10_SEQUENCE=ATGCTCCTATCCTTTTGCCC
PRIMER_RIGHT_10_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_10=634,20
PRIMER_RIGHT_10=1350,20
PRIMER_LEFT_10_TM=60.419
PRIMER_RIGHT_10_TM=59.766
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.2000
PRIMER_RIGHT_10_END_STABILITY=7.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=717
PRIMER_PAIR_11_PENALTY=0.703288
PRIMER_LEFT_11_PENALTY=0.689681
PRIMER_RIGHT_11_PENALTY=0.013607
PRIMER_LEFT_11_SEQUENCE=CCAACAGTTTGTCTCCTGCC
PRIMER_RIGHT_11_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_11=556,20
PRIMER_RIGHT_11=1343,20
PRIMER_LEFT_11_TM=60.690
PRIMER_RIGHT_11_TM=60.014
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=788
PRIMER_PAIR_12_PENALTY=0.703545
PRIMER_LEFT_12_PENALTY=0.470001
PRIMER_RIGHT_12_PENALTY=0.233544
PRIMER_LEFT_12_SEQUENCE=GTCTCCTGCCTTCCTTGAGA
PRIMER_RIGHT_12_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_12=566,20
PRIMER_RIGHT_12=1350,20
PRIMER_LEFT_12_TM=59.530
PRIMER_RIGHT_12_TM=59.766
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=785
PRIMER_PAIR_13_PENALTY=0.793022
PRIMER_LEFT_13_PENALTY=0.156061
PRIMER_RIGHT_13_PENALTY=0.636961
PRIMER_LEFT_13_SEQUENCE=CAGTTTGTCTCCTGCCTTCC
PRIMER_RIGHT_13_SEQUENCE=GAACGGATCCCTGATTTTTG
PRIMER_LEFT_13=560,20
PRIMER_RIGHT_13=1348,20
PRIMER_LEFT_13_TM=59.844
PRIMER_RIGHT_13_TM=59.363
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=789
PRIMER_PAIR_14_PENALTY=0.820832
PRIMER_LEFT_14_PENALTY=0.689681
PRIMER_RIGHT_14_PENALTY=0.131151
PRIMER_LEFT_14_SEQUENCE=CCAACAGTTTGTCTCCTGCC
PRIMER_RIGHT_14_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_14=556,20
PRIMER_RIGHT_14=1349,20
PRIMER_LEFT_14_TM=60.690
PRIMER_RIGHT_14_TM=60.131
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=794
PRIMER_PAIR_15_PENALTY=0.825453
PRIMER_LEFT_15_PENALTY=0.811846
PRIMER_RIGHT_15_PENALTY=0.013607
PRIMER_LEFT_15_SEQUENCE=CCAGAGCTTGAAGAGGCAGA
PRIMER_RIGHT_15_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_15=859,20
PRIMER_RIGHT_15=1343,20
PRIMER_LEFT_15_TM=60.812
PRIMER_RIGHT_15_TM=60.014
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=485
PRIMER_PAIR_16_PENALTY=0.825453
PRIMER_LEFT_16_PENALTY=0.811846
PRIMER_RIGHT_16_PENALTY=0.013607
PRIMER_LEFT_16_SEQUENCE=TCCAGAGCTTGAAGAGGCAG
PRIMER_RIGHT_16_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_16=858,20
PRIMER_RIGHT_16=1343,20
PRIMER_LEFT_16_TM=60.812
PRIMER_RIGHT_16_TM=60.014
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=486
PRIMER_PAIR_17_PENALTY=0.892808
PRIMER_LEFT_17_PENALTY=0.811846
PRIMER_RIGHT_17_PENALTY=0.080962
PRIMER_LEFT_17_SEQUENCE=CCAGAGCTTGAAGAGGCAGA
PRIMER_RIGHT_17_SEQUENCE=AGAAGAATGCACCAAATGCC
PRIMER_LEFT_17=859,20
PRIMER_RIGHT_17=1803,20
PRIMER_LEFT_17_TM=60.812
PRIMER_RIGHT_17_TM=60.081
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=945
PRIMER_PAIR_18_PENALTY=0.892808
PRIMER_LEFT_18_PENALTY=0.811846
PRIMER_RIGHT_18_PENALTY=0.080962
PRIMER_LEFT_18_SEQUENCE=TCCAGAGCTTGAAGAGGCAG
PRIMER_RIGHT_18_SEQUENCE=AGAAGAATGCACCAAATGCC
PRIMER_LEFT_18=858,20
PRIMER_RIGHT_18=1803,20
PRIMER_LEFT_18_TM=60.812
PRIMER_RIGHT_18_TM=60.081
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=9.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=946
PRIMER_PAIR_19_PENALTY=0.923225
PRIMER_LEFT_19_PENALTY=0.689681
PRIMER_RIGHT_19_PENALTY=0.233544
PRIMER_LEFT_19_SEQUENCE=CCAACAGTTTGTCTCCTGCC
PRIMER_RIGHT_19_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_19=556,20
PRIMER_RIGHT_19=1350,20
PRIMER_LEFT_19_TM=60.690
PRIMER_RIGHT_19_TM=59.766
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=7.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=795
PRIMER_PAIR_20_PENALTY=0.926308
PRIMER_LEFT_20_PENALTY=0.912701
PRIMER_RIGHT_20_PENALTY=0.013607
PRIMER_LEFT_20_SEQUENCE=TTGTCTCCTGCCTTCCTTGA
PRIMER_RIGHT_20_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_20=564,20
PRIMER_RIGHT_20=1343,20
PRIMER_LEFT_20_TM=60.913
PRIMER_RIGHT_20_TM=60.014
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=780
PRIMER_PAIR_21_PENALTY=0.932771
PRIMER_LEFT_21_PENALTY=0.156061
PRIMER_RIGHT_21_PENALTY=0.776711
PRIMER_LEFT_21_SEQUENCE=CAGTTTGTCTCCTGCCTTCC
PRIMER_RIGHT_21_SEQUENCE=GGATCCCTGATTTTTGCTCC
PRIMER_LEFT_21=560,20
PRIMER_RIGHT_21=1344,20
PRIMER_LEFT_21_TM=59.844
PRIMER_RIGHT_21_TM=60.777
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=9.4000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=785
PRIMER_PAIR_22_PENALTY=0.942997
PRIMER_LEFT_22_PENALTY=0.811846
PRIMER_RIGHT_22_PENALTY=0.131151
PRIMER_LEFT_22_SEQUENCE=CCAGAGCTTGAAGAGGCAGA
PRIMER_RIGHT_22_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_22=859,20
PRIMER_RIGHT_22=1349,20
PRIMER_LEFT_22_TM=60.812
PRIMER_RIGHT_22_TM=60.131
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=491
PRIMER_PAIR_23_PENALTY=0.942997
PRIMER_LEFT_23_PENALTY=0.811846
PRIMER_RIGHT_23_PENALTY=0.131151
PRIMER_LEFT_23_SEQUENCE=TCCAGAGCTTGAAGAGGCAG
PRIMER_RIGHT_23_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_23=858,20
PRIMER_RIGHT_23=1349,20
PRIMER_LEFT_23_TM=60.812
PRIMER_RIGHT_23_TM=60.131
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.7000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=492
PRIMER_PAIR_24_PENALTY=0.965136
PRIMER_LEFT_24_PENALTY=0.156061
PRIMER_RIGHT_24_PENALTY=0.809075
PRIMER_LEFT_24_SEQUENCE=CAGTTTGTCTCCTGCCTTCC
PRIMER_RIGHT_24_SEQUENCE=ATCTTCTTGCTCCTCGCTGT
PRIMER_LEFT_24=560,20
PRIMER_RIGHT_24=1416,20
PRIMER_LEFT_24_TM=59.844
PRIMER_RIGHT_24_TM=59.191
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=857
PRIMER_PAIR_25_PENALTY=0.982478
PRIMER_LEFT_25_PENALTY=0.156061
PRIMER_RIGHT_25_PENALTY=0.826418
PRIMER_LEFT_25_SEQUENCE=CAGTTTGTCTCCTGCCTTCC
PRIMER_RIGHT_25_SEQUENCE=ACGGATCCCTGATTTTTGCT
PRIMER_LEFT_25=560,20
PRIMER_RIGHT_25=1346,20
PRIMER_LEFT_25_TM=59.844
PRIMER_RIGHT_25_TM=60.826
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=787
PRIMER_PAIR_26_PENALTY=0.999151
PRIMER_LEFT_26_PENALTY=0.985544
PRIMER_RIGHT_26_PENALTY=0.013607
PRIMER_LEFT_26_SEQUENCE=CTCCTGCCTTCCTTGAGAAC
PRIMER_RIGHT_26_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_26=568,20
PRIMER_RIGHT_26=1343,20
PRIMER_LEFT_26_TM=59.014
PRIMER_RIGHT_26_TM=60.014
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=776
PRIMER_PAIR_27_PENALTY=1.003411
PRIMER_LEFT_27_PENALTY=0.366450
PRIMER_RIGHT_27_PENALTY=0.636961
PRIMER_LEFT_27_SEQUENCE=TTTGTCTCCTGCCTTCCTTG
PRIMER_RIGHT_27_SEQUENCE=GAACGGATCCCTGATTTTTG
PRIMER_LEFT_27=563,20
PRIMER_RIGHT_27=1348,20
PRIMER_LEFT_27_TM=60.366
PRIMER_RIGHT_27_TM=59.363
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=786
PRIMER_PAIR_28_PENALTY=1.043852
PRIMER_LEFT_28_PENALTY=0.912701
PRIMER_RIGHT_28_PENALTY=0.131151
PRIMER_LEFT_28_SEQUENCE=TTGTCTCCTGCCTTCCTTGA
PRIMER_RIGHT_28_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_28=564,20
PRIMER_RIGHT_28=1349,20
PRIMER_LEFT_28_TM=60.913
PRIMER_RIGHT_28_TM=60.131
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=7.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=786
PRIMER_PAIR_29_PENALTY=1.045390
PRIMER_LEFT_29_PENALTY=0.811846
PRIMER_RIGHT_29_PENALTY=0.233544
PRIMER_LEFT_29_SEQUENCE=CCAGAGCTTGAAGAGGCAGA
PRIMER_RIGHT_29_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_29=859,20
PRIMER_RIGHT_29=1350,20
PRIMER_LEFT_29_TM=60.812
PRIMER_RIGHT_29_TM=59.766
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=7.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=492
PRIMER_PAIR_30_PENALTY=1.045390
PRIMER_LEFT_30_PENALTY=0.811846
PRIMER_RIGHT_30_PENALTY=0.233544
PRIMER_LEFT_30_SEQUENCE=TCCAGAGCTTGAAGAGGCAG
PRIMER_RIGHT_30_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_30=858,20
PRIMER_RIGHT_30=1350,20
PRIMER_LEFT_30_TM=60.812
PRIMER_RIGHT_30_TM=59.766
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=7.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=493
PRIMER_PAIR_31_PENALTY=1.056066
PRIMER_LEFT_31_PENALTY=0.419105
PRIMER_RIGHT_31_PENALTY=0.636961
PRIMER_LEFT_31_SEQUENCE=ATGCTCCTATCCTTTTGCCC
PRIMER_RIGHT_31_SEQUENCE=GAACGGATCCCTGATTTTTG
PRIMER_LEFT_31=634,20
PRIMER_RIGHT_31=1348,20
PRIMER_LEFT_31_TM=60.419
PRIMER_RIGHT_31_TM=59.363
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=11.2000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=715
PRIMER_PAIR_32_PENALTY=1.067876
PRIMER_LEFT_32_PENALTY=1.054269
PRIMER_RIGHT_32_PENALTY=0.013607
PRIMER_LEFT_32_SEQUENCE=GAATGCTCCTATCCTTTTGCC
PRIMER_RIGHT_32_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_32=632,21
PRIMER_RIGHT_32=1343,20
PRIMER_LEFT_32_TM=60.054
PRIMER_RIGHT_32_TM=60.014
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=10.0000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=712
PRIMER_PAIR_33_PENALTY=1.071994
PRIMER_LEFT_33_PENALTY=1.058387
PRIMER_RIGHT_33_PENALTY=0.013607
PRIMER_LEFT_33_SEQUENCE=CGTTGTGGAACACTTCAACCT
PRIMER_RIGHT_33_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_33=373,21
PRIMER_RIGHT_33=1343,20
PRIMER_LEFT_33_TM=60.058
PRIMER_RIGHT_33_TM=60.014
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=971
PRIMER_PAIR_34_PENALTY=1.093473
PRIMER_LEFT_34_PENALTY=1.079866
PRIMER_RIGHT_34_PENALTY=0.013607
PRIMER_LEFT_34_SEQUENCE=GCTCCTATCCTTTTGCCCTG
PRIMER_RIGHT_34_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_34=636,20
PRIMER_RIGHT_34=1343,20
PRIMER_LEFT_34_TM=61.080
PRIMER_RIGHT_34_TM=60.014
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=708
PRIMER_PAIR_35_PENALTY=1.093473
PRIMER_LEFT_35_PENALTY=1.079866
PRIMER_RIGHT_35_PENALTY=0.013607
PRIMER_LEFT_35_SEQUENCE=TGCTCCTATCCTTTTGCCCT
PRIMER_RIGHT_35_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_35=635,20
PRIMER_RIGHT_35=1343,20
PRIMER_LEFT_35_TM=61.080
PRIMER_RIGHT_35_TM=60.014
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=10.9000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=709
PRIMER_PAIR_36_PENALTY=1.104847
PRIMER_LEFT_36_PENALTY=1.091240
PRIMER_RIGHT_36_PENALTY=0.013607
PRIMER_LEFT_36_SEQUENCE=GCCTTCCTTGAGAACTTTGG
PRIMER_RIGHT_36_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_36=573,20
PRIMER_RIGHT_36=1343,20
PRIMER_LEFT_36_TM=58.909
PRIMER_RIGHT_36_TM=60.014
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.8000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=771
PRIMER_PAIR_37_PENALTY=1.106961
PRIMER_LEFT_37_PENALTY=0.470001
PRIMER_RIGHT_37_PENALTY=0.636961
PRIMER_LEFT_37_SEQUENCE=GTCTCCTGCCTTCCTTGAGA
PRIMER_RIGHT_37_SEQUENCE=GAACGGATCCCTGATTTTTG
PRIMER_LEFT_37=566,20
PRIMER_RIGHT_37=1348,20
PRIMER_LEFT_37_TM=59.530
PRIMER_RIGHT_37_TM=59.363
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=7.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=783
PRIMER_PAIR_38_PENALTY=1.116696
PRIMER_LEFT_38_PENALTY=0.985544
PRIMER_RIGHT_38_PENALTY=0.131151
PRIMER_LEFT_38_SEQUENCE=CTCCTGCCTTCCTTGAGAAC
PRIMER_RIGHT_38_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_38=568,20
PRIMER_RIGHT_38=1349,20
PRIMER_LEFT_38_TM=59.014
PRIMER_RIGHT_38_TM=60.131
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=782
PRIMER_PAIR_39_PENALTY=1.143161
PRIMER_LEFT_39_PENALTY=0.366450
PRIMER_RIGHT_39_PENALTY=0.776711
PRIMER_LEFT_39_SEQUENCE=TTTGTCTCCTGCCTTCCTTG
PRIMER_RIGHT_39_SEQUENCE=GGATCCCTGATTTTTGCTCC
PRIMER_LEFT_39=563,20
PRIMER_RIGHT_39=1344,20
PRIMER_LEFT_39_TM=60.366
PRIMER_RIGHT_39_TM=60.777
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=9.4000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=782
PRIMER_PAIR_40_PENALTY=1.146245
PRIMER_LEFT_40_PENALTY=0.912701
PRIMER_RIGHT_40_PENALTY=0.233544
PRIMER_LEFT_40_SEQUENCE=TTGTCTCCTGCCTTCCTTGA
PRIMER_RIGHT_40_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_40=564,20
PRIMER_RIGHT_40=1350,20
PRIMER_LEFT_40_TM=60.913
PRIMER_RIGHT_40_TM=59.766
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=787
PRIMER_PAIR_41_PENALTY=1.175526
PRIMER_LEFT_41_PENALTY=0.366450
PRIMER_RIGHT_41_PENALTY=0.809075
PRIMER_LEFT_41_SEQUENCE=TTTGTCTCCTGCCTTCCTTG
PRIMER_RIGHT_41_SEQUENCE=ATCTTCTTGCTCCTCGCTGT
PRIMER_LEFT_41=563,20
PRIMER_RIGHT_41=1416,20
PRIMER_LEFT_41_TM=60.366
PRIMER_RIGHT_41_TM=59.191
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=854
PRIMER_PAIR_42_PENALTY=1.185421
PRIMER_LEFT_42_PENALTY=1.054269
PRIMER_RIGHT_42_PENALTY=0.131151
PRIMER_LEFT_42_SEQUENCE=GAATGCTCCTATCCTTTTGCC
PRIMER_RIGHT_42_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_42=632,21
PRIMER_RIGHT_42=1349,20
PRIMER_LEFT_42_TM=60.054
PRIMER_RIGHT_42_TM=60.131
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=45.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=10.0000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=718
PRIMER_PAIR_43_PENALTY=1.189538
PRIMER_LEFT_43_PENALTY=1.058387
PRIMER_RIGHT_43_PENALTY=0.131151
PRIMER_LEFT_43_SEQUENCE=CGTTGTGGAACACTTCAACCT
PRIMER_RIGHT_43_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_43=373,21
PRIMER_RIGHT_43=1349,20
PRIMER_LEFT_43_TM=60.058
PRIMER_RIGHT_43_TM=60.131
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=7.6000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=977
PRIMER_PAIR_44_PENALTY=1.192868
PRIMER_LEFT_44_PENALTY=0.366450
PRIMER_RIGHT_44_PENALTY=0.826418
PRIMER_LEFT_44_SEQUENCE=TTTGTCTCCTGCCTTCCTTG
PRIMER_RIGHT_44_SEQUENCE=ACGGATCCCTGATTTTTGCT
PRIMER_LEFT_44=563,20
PRIMER_RIGHT_44=1346,20
PRIMER_LEFT_44_TM=60.366
PRIMER_RIGHT_44_TM=60.826
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=784
PRIMER_PAIR_45_PENALTY=1.195815
PRIMER_LEFT_45_PENALTY=0.419105
PRIMER_RIGHT_45_PENALTY=0.776711
PRIMER_LEFT_45_SEQUENCE=ATGCTCCTATCCTTTTGCCC
PRIMER_RIGHT_45_SEQUENCE=GGATCCCTGATTTTTGCTCC
PRIMER_LEFT_45=634,20
PRIMER_RIGHT_45=1344,20
PRIMER_LEFT_45_TM=60.419
PRIMER_RIGHT_45_TM=60.777
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=11.2000
PRIMER_RIGHT_45_END_STABILITY=9.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=711
PRIMER_PAIR_46_PENALTY=1.209605
PRIMER_LEFT_46_PENALTY=1.195998
PRIMER_RIGHT_46_PENALTY=0.013607
PRIMER_LEFT_46_SEQUENCE=TTCCAGAGCTTGAAGAGGCA
PRIMER_RIGHT_46_SEQUENCE=GATCCCTGATTTTTGCTCCA
PRIMER_LEFT_46=857,20
PRIMER_RIGHT_46=1343,20
PRIMER_LEFT_46_TM=61.196
PRIMER_RIGHT_46_TM=60.014
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=9.7000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=487
PRIMER_PAIR_47_PENALTY=1.211017
PRIMER_LEFT_47_PENALTY=1.079866
PRIMER_RIGHT_47_PENALTY=0.131151
PRIMER_LEFT_47_SEQUENCE=GCTCCTATCCTTTTGCCCTG
PRIMER_RIGHT_47_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_47=636,20
PRIMER_RIGHT_47=1349,20
PRIMER_LEFT_47_TM=61.080
PRIMER_RIGHT_47_TM=60.131
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.7000
PRIMER_RIGHT_47_END_STABILITY=7.6000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=714
PRIMER_PAIR_48_PENALTY=1.211017
PRIMER_LEFT_48_PENALTY=1.079866
PRIMER_RIGHT_48_PENALTY=0.131151
PRIMER_LEFT_48_SEQUENCE=TGCTCCTATCCTTTTGCCCT
PRIMER_RIGHT_48_SEQUENCE=GGAACGGATCCCTGATTTTT
PRIMER_LEFT_48=635,20
PRIMER_RIGHT_48=1349,20
PRIMER_LEFT_48_TM=61.080
PRIMER_RIGHT_48_TM=60.131
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=10.9000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=715
PRIMER_PAIR_49_PENALTY=1.219088
PRIMER_LEFT_49_PENALTY=0.985544
PRIMER_RIGHT_49_PENALTY=0.233544
PRIMER_LEFT_49_SEQUENCE=CTCCTGCCTTCCTTGAGAAC
PRIMER_RIGHT_49_SEQUENCE=AGGAACGGATCCCTGATTTT
PRIMER_LEFT_49=568,20
PRIMER_RIGHT_49=1350,20
PRIMER_LEFT_49_TM=59.014
PRIMER_RIGHT_49_TM=59.766
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=7.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=783
=
SEQUENCE_ID=MAGIC.149_5_12706514_12706521_wil-2_ler-0_331584_25052_TRUE
SEQUENCE_TARGET=950,107
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTANGNAAGNTNTCTNANCNNTNAAANTCNTNGGAAAAGGTAANNCATAATCACCANGANGATTNCTNCNTNNNCAAGAAANTGNTGTTTCTGCNAATTCNTGAAACCCTNNNNNNNNAGATTCAATGTGTCATCTTTNNNNNNNNNNNNNNNNNTCTNNACNNNNNCTNNNNNNNNNNNNNNNTTNTGGGCAGTTTCNTGGCNCTATAGGGTNGANAATNAACNNTNNGATTAANAAGTGAANNGTCCATNNTNAAGTNACTAGNGNNNTTTNGTNNCANTTAANTGAGTTGTTCTTCNTCTTNAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTTNAATTNATTACTTGAATTTTAAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNANATGAANANATTGTTATTNNNANNAAAAAANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCCAAAAGTGATGTCTTCCCTNTTGGTTNTGACTCACAAATGGTTNAATNTCANNATTCTCTCCAAAATTATCGATNNTTTTTCAATCTCGTACACATTCATTAGTATATGTANACGATTCTTTTAATTAACTGAATTTTCATATTAGCTCCACGGTAAGTAGAGTGTCACCAACATCTCTTACACNTCATGTCCCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_15667240_15667749_ws-0_zu-0_22457_13174_TRUE
SEQUENCE_TARGET=950,609
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_5_14266734_14266962_oy-0_kn-0_1348610_57127_TRUE
SEQUENCE_TARGET=950,328
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCATGTAACNTAANGTGACNNATNAATAATGTAGAGAATAACGNTAGNCGTCATANCTACGGTTTGGATCGGATTTACTCTTNCAAACANTANACNNNNNNNNNNNNNTTCGGATAGCCAATAATTTTAAGANAAAAACTNNTCGCTAAGAANNNNNNNNTNNCACNCTTTAGATGGGTGCTCTTCCTANATNTTTTTTTTGGGTAGGATTTATGGTCTCAGTCCTACANCATTANCAAGGGTNATAANTTTTCATAAGAANNAAANAAAGTTGTGCNNNNTTTCAACAATTCTNCAACGTTCTAATCAAAAGGGCCAATGCANATGAATGAAGNNNNNNNNNNNNNNNNNNNNNNNNGGTTAAGTTTAGGNTCTTAGCCGNNAAAGCAGTCTAATACTTAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGTTGGATTGAAANGTTTGGAGAGACAAGTCATTGGCCAGAGTGGGGTTGGANCTCTANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_9029498_9030223_ler-0_po-0_14015_5685_TRUE
SEQUENCE_TARGET=950,825
SEQUENCE_TEMPLATE=GCTTAGTACCGGCGGCTACTGCAAAANGGATTGTNTTCTTCTTAGGCTTGATACTTGCCTTGGGAAGAATANAACNATCNTTTTGCGGTAGCTAAAACGCTAANCTCTATCGTCATATCTTTNTCATTNATACTTATTTGATATCTNAATATCATAAAAATGGCCGCTCGTTCANTCTCCGGTGCCGTTAAATCTCTTTGCTNCGCCGCATCCGGAAGTCTGTCTNGTTCCATTGTCTTAAGGTACTTATGTTTGAANTGATCCCATTTTTTCANGTTACAANATATAAACATTTACACACTCTTTCATTTGCTTAGATCGTATAAAGAATTTATAATTCTAATTGAATAATAGTTGATNGTTTGAATTTGAGTGAAGGCTAAAGATTGATCTATCATTAATAAACGTAATTANTNTACTTAGATCTATTAAAGATAANTATGCATGATANTATCACGTTNTTTTCTACGTTTTCGTTATGTTATTTAGTCATTTGCTGTTTATATAATCTTACCTTCAAAATAAATTATTGGAATGTAGGAGGAGTTACGTTGCTACATCGCAGAATGTAACAGCAGCAGGATTGAGTAAGGGAGGTTCCACCAGAGTNATGGTGGGGAAGATGGAACANAGAGGTTTAGATCAAGAGGCAGAGTCTGCTTGGGGACCAGATCCAGTTACTGGATACTANAGACCTTCCAATCGTGCGGCTGANATTGATCCAGCTGAGCTCAGAGANTTGCTTTTGAAAAACAAAGCAAAGTCTTTCTGAGNATTTTGATTGGTTGGTTGACTTNNNNNNNNNNNNNGGTTGTCGTAGGCGACNATTNTGATTATGTGTGTTTATGTTTGTCTTGTATCAACTATTAAGTAAAAATAAAGATCACATTAACTAGTTAAAAATATAATANCTGTTTGGATCGACTAACACAACATCTTTAGTATANNNGNNTCTTAAATGTTTTAAGAGATAAATGGGGATATACGAAANATAGAAAACNTTNCTAATTATGACTAAATTNTGATANTTAACAACGTNAACAACAACAAAACAAGATNATTTCACTATGGCATTATATTTTGTGACCACAATCTTATACTTNTTGTTGTTGTCAACATCAAATACAACAANAGAAAATGATGGTGCTTTTTCATNCCCCNTATAAAATTTTGTTGATTNTTCATGCCCATANTTTTGNTGAGGCTGCTAATGNCCTAATAGTCTTAGAAAATGCAAANGNACTNTTGAGGGTTGAGAANATGCTGTANTTCGNCCCACTTCTGGNCTAGTCGAGTGAATACGTAAGGATCAATCATCAATACTNNGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTCTCAACATTTNNCNGTCTAAACGTGTTGTCACTCACATTTCTTCCCCCAACAAATATCAAACATGTTGTCACTCTCGTTNNCATNTATGNAATTGANCATCAACAAGTATTGAGGAGGACATATCCCNGAGCTAGTCATTATGACCATGTTNTTNTGTCTGAAATGAANATCACGATCCGAACGCTAAAGGGAAANGTCAAANTCCGAGAGACNAGTAAAACNGAAANATGTTTGCTCCACAANTACNGAACCGTACGNACGTNNNNNNNNCTGTANACGCTCACTTGCAATCGAACNNACACAACGACACANNACNTTTTTTTACGNNTNANANNTNACAAANTCAACCGTCANGAATCTCNAAACTTATCCAAACAAAAAAGTTACTAAGCCTCAAAACNTAGTAAACACTCCATTCTCTNATCTTCGCTTAACAAAAGATGCNTTACAATAACAANCCCTAAAATATGTAACATCTNCAAATNAATCTAATTTTGTCCAAAAAAATAACGTACACAAAATCNNACACCACAATAATGTAACAAAAAATATAAAGANTTGTATTTATAAGACCCAAATTAATTAACAAGCTCTTGNCNNANAAAAAAAGGNATTTAAGAGAGGAGAGGTNCAACACGTNATGTGAGGCCTTCCTCTTCATCTNGAGNAATCTCANNNNNNNNNNNNCTTAAACCAACCGGTTTGGCATTTCTGTAAATTCCATAAAGTATGAGTTGCATTGTCCCAAACACAAACCCCACTCCATTTGGCACCTATCCAATTTCACCACANAACTTTAATTAACTAAATTAANCAATCAATCACAAATACATTATTTCTCAACAACATAATTATAATCAACGCCTANCCGAAATCACAATAAAACGCATTTGNAAATGTCCCCACAAAATAGTNGTTGTTGCATTTTTGAACCAGACACGNTCTTTAAAAAANTAATTGTTTGNAAAAAATGGCCNAAAAAAANAANATTTTGGTTAATGTTGGAGACNTGGAGTCCNCAATTTTTNNAAAATCATTGAGCATTAAATCGATNTTTTCNTTTCCAGAAGATTTTTTATAAACTGTGACNTGACC
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=7.107481
PRIMER_LEFT_0_PENALTY=4.189810
PRIMER_RIGHT_0_PENALTY=2.917671
PRIMER_LEFT_0_SEQUENCE=GTTTGGATCGACTAACACAACATC
PRIMER_RIGHT_0_SEQUENCE=CCTTTAGCGTTCGGATCGTG
PRIMER_LEFT_0=913,24
PRIMER_RIGHT_0=1819,20
PRIMER_LEFT_0_TM=59.810
PRIMER_RIGHT_0_TM=62.918
PRIMER_LEFT_0_GC_PERCENT=41.667
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.3000
PRIMER_RIGHT_0_END_STABILITY=8.4000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=907
PRIMER_PAIR_1_PENALTY=7.313788
PRIMER_LEFT_1_PENALTY=4.396117
PRIMER_RIGHT_1_PENALTY=2.917671
PRIMER_LEFT_1_SEQUENCE=TTGGATCGACTAACACAACATC
PRIMER_RIGHT_1_SEQUENCE=CCTTTAGCGTTCGGATCGTG
PRIMER_LEFT_1=915,22
PRIMER_RIGHT_1=1819,20
PRIMER_LEFT_1_TM=57.604
PRIMER_RIGHT_1_TM=62.918
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=905
PRIMER_PAIR_2_PENALTY=7.884795
PRIMER_LEFT_2_PENALTY=4.967124
PRIMER_RIGHT_2_PENALTY=2.917671
PRIMER_LEFT_2_SEQUENCE=TGGATCGACTAACACAACATC
PRIMER_RIGHT_2_SEQUENCE=CCTTTAGCGTTCGGATCGTG
PRIMER_LEFT_2=916,21
PRIMER_RIGHT_2=1819,20
PRIMER_LEFT_2_TM=56.033
PRIMER_RIGHT_2_TM=62.918
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=904
PRIMER_PAIR_3_PENALTY=7.893098
PRIMER_LEFT_3_PENALTY=4.975427
PRIMER_RIGHT_3_PENALTY=2.917671
PRIMER_LEFT_3_SEQUENCE=TTGGATCGACTAACACAACA
PRIMER_RIGHT_3_SEQUENCE=CCTTTAGCGTTCGGATCGTG
PRIMER_LEFT_3=915,20
PRIMER_RIGHT_3=1819,20
PRIMER_LEFT_3_TM=55.025
PRIMER_RIGHT_3_TM=62.918
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=905
PRIMER_PAIR_4_PENALTY=11.974478
PRIMER_LEFT_4_PENALTY=9.056807
PRIMER_RIGHT_4_PENALTY=2.917671
PRIMER_LEFT_4_SEQUENCE=CTGTTTGGATCGACTAACACAACATC
PRIMER_RIGHT_4_SEQUENCE=CCTTTAGCGTTCGGATCGTG
PRIMER_LEFT_4=911,26
PRIMER_RIGHT_4=1819,20
PRIMER_LEFT_4_TM=63.057
PRIMER_RIGHT_4_TM=62.918
PRIMER_LEFT_4_GC_PERCENT=42.308
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=909
=
SEQUENCE_ID=MAGIC.446_5_18157240_18158000_hi-0_po-0_8563_7340_TRUE
SEQUENCE_TARGET=950,860
SEQUENCE_TEMPLATE=ATTGGTNTCTTTAAAGCTTGTATGCGTAGAGCTTGAGAATCCAAAGTTTGTTGTTTCTCTACCTTGTCTCAAGATCATGCATCTAGAAAATAATTGGTACGGTTATGATGCTCCTTTGNTTGTGGAGAANCTCANCNCAGGGTGTCNTGTTCTTGAAGATCTTACCGTGGTAAGGATTCATGATATGGTAATACCAGATCTGCCTCTTCTACATGTGAAGTCTCTGACACTGAAGATCTTANNTTNNGNNNNTGANTTGGAGATGATGACTACNATACCTATTNCGGTGGAGATTGANGCTCCAAAANTAGAGTNTATGAGTTTTATAANTAGGCACTCTGATANAATCATGGTAAAGAATCTGAGTTCATTGTTCAAGTTAAATCTTGATACTGAATTTGGCNNNNNNNNNNNNNNNNNNNNAGATATTATCCGTGACGTTCTCACTGGTATNTCNAGTGTAAGACAGATGATCATCTCTCAANNTACTCTANNGGTAAATAATGNTTCTCTGTGATTAACAATNTANTTTTTNTAGCTATGGAANGATTNCTCACNTAATAACATTCTNTTTCTCTTTTGCAGGTCATATNTCGTTATTCAAAACTCGGNNNNNNNNNNNNNNNNNNNNACCTATCTCATTTGAANGCTGCATTCTCCAGNACATCNTTACAACTATTGCCCGCCTTTCTTNAGAGCTGCCCAAANCTNAAAAANCTCATCTTGGTANGAATCTGTCTTTGATCTTTGATTTGGCNNATTAAGATCATTGTTGCATNTATTGTAAGCTGATTAGNTCNNNNNNNNNTGNAGGACTTATNTNTTTCNGCGGAGCCAGAGNNAANCGATNTTACCAATGTGCCTCGNTGTANAACATCGACTNTGGAGTGTATTGAGATTAANAANCTGATTAGGAAGGAAGCAACTGGGGTNAAACTAGTTCANTNTTTTCTTGAGAATTCACCAATCCTTAAGAAACTCAAGCNGAGTTTCNCANATTNTCCTATGAGNNNTATGACCAATCTACCGTTAGATCATCTCTATAAAATGTATCTTACNNCCAGAAAGCGTTCTCGCAGATGNCAAGTTATNATTTCTNAACATGNATCTGATCATNTCANTGAGGGCATGAGCATTGTCATTGAAGANCAATGTNGAAGAATAATCTTATGAAAGTTGTAGTCAGGCTTAGCATTCTGTAGTTGGCACACAAATNATANTGTCNTCTNCAGATTTGGTNATCTCACTTACATGCTTAAAGGNCAGATTANGNNACNTTTCTAGTTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTTCTATCATTTATAAAGGATTGATGAAATGTTTTTGTTGTTTCTAAGACATCTTATTTTATTAATTAGAATGTTGTCAATACATAATTTAGTTTGGGCAATCGGGCAGAGTTTGGCCTTGTTTGCGGCATACACATTTTCCNTTTACGCAACACGCCCGACGATAATCAGNACATGCCANCCCTCCACACTCAAGGTATGTGTCACATATCCCTCCATGATTCACNTCGATTNTTTTTATATTCCCTTCTTCTAAATTTATCCTTGACGGATTAGGAAGACATTTGCTGTCNTTACATTCTTTGTCTTCCTTAGCCTCGATNAGTTTACTCTGATCTGTNGTTTATTTCAAGAAAAACAATGNTTTCAAATTAGTATTCTCTATGTTTTCAAAAATNGATTTAAGTANCGTATACGAATATAATGGNNACATAGATTATNCNAAATNAAAAAAAAGNTTGAGTTTAACCACTACNCCATTTTATGAATTCCCAAATCAAAATTCAAATTTACTTACAAGAGAAGGACNNNAACAAGAGGGAGAGAAGAAAAGCNGTCTTAAAATAAAGGTTGNTCATAGCTAACAATTGTCTGTATTTGAATNTTNGATGATNGTTGTGNAAAGAGNATTNTATAGCNTTGCGTTCATTTNTATATGGGATGAAANAACAGNNTTTNTTTTTTNTATACAACTGNAACTTTAAATTTTAGTACTATAATTCTACATAGATCGGATANGAACNAACTTTTTTTNTTTCNTCAGTTATGNCTAATTCAANCAAGATATGAAAGTCTATTTCCCCAAAANCAATTNTGGTAATAAATCCACAATTTAATTTGGGATTTGTCCTTTCTACTACTTTTNAAAATATCATTTCCNAANNTANATNNNNNNNNNNNNNNNTGAGNTGGAAAAACAGTTTTCATNTTTTACTTTCTATGAAAATTTAACTTTANANNTTNGANTTTNTCAGTTATGAACCTCANTNGNATCCAAGATANGAANGTCTNTTNCCNAAAAAAANAANTNTTTGGGATATNTGNGCTTTCTACTANTTNTGAAAAGATTATCTCCAAATCTACGACATTTCATAATTTCATAATTCCNTTCCATTTCTATGAAAGTGTTGTACCNCAANNNNTTTTTTTGGATGAAACNATNGAGGTATATTCTTGAGAATTATGTAGTNCTCAAGAAACTCATTTTAAGTTTATATTCTATAACCNAACAAACCTTTGATACNTCCANGGATCTTTTTACATTCCNTTTAACATTTAATTNGATAATAATTAATAGGTAGTANAGATTTATNGAGATAGATGCTCCGACTTAAGTACATGAGTTTAAGAGATAGTANATCCGATAGGGTNGTTGTAAAGGATGTGANTTCATTGGT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_13105235_13105256_ws-0_can-0_152336_20359_TRUE
SEQUENCE_TARGET=950,121
SEQUENCE_TEMPLATE=ATATATTTTAGGGACCTATTATAGATTCTCAAAATATTACAAAGACCTCAACCACATTCACACAAAACTTTTGATCTTTGTTCAAGAATACATTATCTTCCCATGTCTTACGTGAATCATTGCAAATTAAGAATTTACAAGTAATTTNTAATANAAGACACTCCATTCTTGGTNATTTTTTTNAAAAGATCTTAGTGTTTTTCATTATTAACACACTTTNCTTTTTGTATACTATATTNTAGGATGAGGAAGAGAAAAGATGGTGCGTCAAATGATGAATCAAAGAATACGAATAACCATCGTCATATGTCAGATATTGAGAATGAAAATCCCAATCCTAANCAGAGTGCTAGCAGTATTCNCNNCCATNTTCCAAACAAGCAAATTTTTGGATAATATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_2_12294274_12294462_kn-0_tsu-0_55068_1099748_TRUE
SEQUENCE_TARGET=950,288
SEQUENCE_TEMPLATE=ACAAACAAAAATATTGTGAGNCGCNTTNCCATAAANATGTTATTATAAANTAGGATACAANGGAGAAAATAGAGGCGGGNAAAACAAAAGNACTGACTATAAAATAAAACAAAGAAAAATCACATTTGTAGTGGGATGCCTTTCCATTAGGAAAACGACTCTCCGANGAGGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCCACTAAATCATTAATGAAGGAATCAATANGTAANCANCTAATCAAATAACAAACNATAGTCTNTANGAACTATGACGCAGNGGATATTAAAATGGANATTTATATTATATTNTTGTTATTGTTANAATNNNNNNNNNNNNNNNNNNNCTTANTAAAACTNCGTTCTCAAGAGTNACTNTTNAANNNNNTTTTNAATGTGAACAATGGTTAGTGTTAATTACACGGACTTGCTTTCGCTTTATTGGCCGTCCATNAATCTGCTTTTGAGGAATGGGTTTTTCTTTANTTAGTTATCAATTCCTTGCTTTGAGGATTCTACTTACNAAATTTAATATTATANGATTTCTATTTACAAGATGTTNTNGTNAAAAGNNTGAAAATTAAANAAATTACANATTATATACACATATATANATNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAAGAAGAATGGNAAGAACTCCATTGGNTTACTCGTATTCTNTGTTTGACTTTNNNNNNNNNNNNTATATATANTCTTAAAGGCGGCTATTTAGAAATCTCGAATAANNNNNNNNNNNTAGTTTCTANTTTCTACGTTCTNNTAAAAGTNTNTATTCATGANTAGAAACTGATGGCCCATTGNGTTGATATTAATCTACAAAGAAGGCTCGTAAANNTGGCCCATNAGTNNNTNNTTNNAANNGANTNAGGNGNNANNGNGAGTCCNCCCAAATTCNAGAACCTGTTTTGAACATTTATTNAGTCAGTNATTATATTTTGGTTATGAGATTAGTGGCTANGTTCGAAGTTAGTAAGTTAGTGANAGTAATCATTGTAGNAAAAGTTGAATCCAAGCTTTTTCAACAANAGATTGTCCCCACCTTGAGACACTAGTACCTAGTGGGTANATACGAATTCGATGTACAATACCATTCNTTTTCTTCTTCTTTNTTTTTCCANTATATATTNATTNTGATTAATATTGATCAGACTNCTTACTGCATNTGCATGTGTTGATTAGTCANCTTTATTAAANNTATTTNATTTTNGACTTAGGCTCACTAACCCTANGGATTGNTCTTCTTATTTANTTTTTTTGCATGTTCTCATTTGANNAGAGCAAAAANCGACAAAGAAGANAATNTTTTTTGTTAAAACTCTGAATCNGATTATNNNNNNNNNTTTTAACGACAAAANATGGANGTAGTTTTTACACTANNCATCANTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTATNGTAACTTTGTTNGGTTTCTTTTTCTTATTTAATATAATACATCAAATTTTGGGACAATAAAAAATCAAATAAAGTAAAATAGCAATTTAGAAGGTGNAGATTCAAGATCGATCCAAGAAATGCTTTTCACACACTCCTTCTCATNAGATAGGACCCATNCTATAATAATAATATCTACTTTACCTTAACAAAACAATTTNAAAATAAATGATTCGTTACCTTCCTCTTTAAAACTCGTATTAGTAGCAATTAGTCGCATAATCACACGACCAACTTTCCCTTAAAATCAACAGACAGAAAGTGAGTGTTATAGACTTANAGTGAGANAGAGATAAAGATAGANNNNNNNNNNNNAAAAAGAGAGAATGAGANTTAGTGATATTTTCTGCTCTTCTCCGGCATCAACCG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.824360
PRIMER_LEFT_0_PENALTY=0.329550
PRIMER_RIGHT_0_PENALTY=0.494811
PRIMER_LEFT_0_SEQUENCE=TCTGCTTTTGAGGAATGGGT
PRIMER_RIGHT_0_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_0=467,20
PRIMER_RIGHT_0=1386,20
PRIMER_LEFT_0_TM=59.670
PRIMER_RIGHT_0_TM=59.505
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=9.4000
PRIMER_RIGHT_0_END_STABILITY=6.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=920
PRIMER_PAIR_1_PENALTY=0.948857
PRIMER_LEFT_1_PENALTY=0.454047
PRIMER_RIGHT_1_PENALTY=0.494811
PRIMER_LEFT_1_SEQUENCE=TGCTTTTGAGGAATGGGTTT
PRIMER_RIGHT_1_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_1=469,20
PRIMER_RIGHT_1=1386,20
PRIMER_LEFT_1_TM=59.546
PRIMER_RIGHT_1_TM=59.505
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=918
PRIMER_PAIR_2_PENALTY=0.970468
PRIMER_LEFT_2_PENALTY=0.329550
PRIMER_RIGHT_2_PENALTY=0.640918
PRIMER_LEFT_2_SEQUENCE=TCTGCTTTTGAGGAATGGGT
PRIMER_RIGHT_2_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_2=467,20
PRIMER_RIGHT_2=1387,20
PRIMER_LEFT_2_TM=59.670
PRIMER_RIGHT_2_TM=59.359
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=921
PRIMER_PAIR_3_PENALTY=1.094964
PRIMER_LEFT_3_PENALTY=0.454047
PRIMER_RIGHT_3_PENALTY=0.640918
PRIMER_LEFT_3_SEQUENCE=TGCTTTTGAGGAATGGGTTT
PRIMER_RIGHT_3_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_3=469,20
PRIMER_RIGHT_3=1387,20
PRIMER_LEFT_3_TM=59.546
PRIMER_RIGHT_3_TM=59.359
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=919
PRIMER_PAIR_4_PENALTY=1.324372
PRIMER_LEFT_4_PENALTY=0.829561
PRIMER_RIGHT_4_PENALTY=0.494811
PRIMER_LEFT_4_SEQUENCE=CTGCTTTTGAGGAATGGGTT
PRIMER_RIGHT_4_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_4=468,20
PRIMER_RIGHT_4=1386,20
PRIMER_LEFT_4_TM=59.170
PRIMER_RIGHT_4_TM=59.505
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=919
PRIMER_PAIR_5_PENALTY=1.369310
PRIMER_LEFT_5_PENALTY=0.874500
PRIMER_RIGHT_5_PENALTY=0.494811
PRIMER_LEFT_5_SEQUENCE=ATCTGCTTTTGAGGAATGGG
PRIMER_RIGHT_5_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_5=466,20
PRIMER_RIGHT_5=1386,20
PRIMER_LEFT_5_TM=59.126
PRIMER_RIGHT_5_TM=59.505
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.6000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=921
PRIMER_PAIR_6_PENALTY=1.458519
PRIMER_LEFT_6_PENALTY=0.963709
PRIMER_RIGHT_6_PENALTY=0.494811
PRIMER_LEFT_6_SEQUENCE=TACACGGACTTGCTTTCGCT
PRIMER_RIGHT_6_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_6=429,20
PRIMER_RIGHT_6=1386,20
PRIMER_LEFT_6_TM=60.964
PRIMER_RIGHT_6_TM=59.505
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=9.9000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=958
PRIMER_PAIR_7_PENALTY=1.470479
PRIMER_LEFT_7_PENALTY=0.829561
PRIMER_RIGHT_7_PENALTY=0.640918
PRIMER_LEFT_7_SEQUENCE=CTGCTTTTGAGGAATGGGTT
PRIMER_RIGHT_7_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_7=468,20
PRIMER_RIGHT_7=1387,20
PRIMER_LEFT_7_TM=59.170
PRIMER_RIGHT_7_TM=59.359
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=920
PRIMER_PAIR_8_PENALTY=1.515417
PRIMER_LEFT_8_PENALTY=0.874500
PRIMER_RIGHT_8_PENALTY=0.640918
PRIMER_LEFT_8_SEQUENCE=ATCTGCTTTTGAGGAATGGG
PRIMER_RIGHT_8_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_8=466,20
PRIMER_RIGHT_8=1387,20
PRIMER_LEFT_8_TM=59.126
PRIMER_RIGHT_8_TM=59.359
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=922
PRIMER_PAIR_9_PENALTY=1.540642
PRIMER_LEFT_9_PENALTY=1.045831
PRIMER_RIGHT_9_PENALTY=0.494811
PRIMER_LEFT_9_SEQUENCE=ATCTGCTTTTGAGGAATGGGT
PRIMER_RIGHT_9_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_9=466,21
PRIMER_RIGHT_9=1386,20
PRIMER_LEFT_9_TM=59.954
PRIMER_RIGHT_9_TM=59.505
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=921
PRIMER_PAIR_10_PENALTY=1.604626
PRIMER_LEFT_10_PENALTY=0.963709
PRIMER_RIGHT_10_PENALTY=0.640918
PRIMER_LEFT_10_SEQUENCE=TACACGGACTTGCTTTCGCT
PRIMER_RIGHT_10_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_10=429,20
PRIMER_RIGHT_10=1387,20
PRIMER_LEFT_10_TM=60.964
PRIMER_RIGHT_10_TM=59.359
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.9000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=959
PRIMER_PAIR_11_PENALTY=1.686749
PRIMER_LEFT_11_PENALTY=1.045831
PRIMER_RIGHT_11_PENALTY=0.640918
PRIMER_LEFT_11_SEQUENCE=ATCTGCTTTTGAGGAATGGGT
PRIMER_RIGHT_11_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_11=466,21
PRIMER_RIGHT_11=1387,20
PRIMER_LEFT_11_TM=59.954
PRIMER_RIGHT_11_TM=59.359
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=922
PRIMER_PAIR_12_PENALTY=1.694029
PRIMER_LEFT_12_PENALTY=0.329550
PRIMER_RIGHT_12_PENALTY=1.364479
PRIMER_LEFT_12_SEQUENCE=TCTGCTTTTGAGGAATGGGT
PRIMER_RIGHT_12_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_12=467,20
PRIMER_RIGHT_12=1387,21
PRIMER_LEFT_12_TM=59.670
PRIMER_RIGHT_12_TM=60.364
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=921
PRIMER_PAIR_13_PENALTY=1.818526
PRIMER_LEFT_13_PENALTY=0.454047
PRIMER_RIGHT_13_PENALTY=1.364479
PRIMER_LEFT_13_SEQUENCE=TGCTTTTGAGGAATGGGTTT
PRIMER_RIGHT_13_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_13=469,20
PRIMER_RIGHT_13=1387,21
PRIMER_LEFT_13_TM=59.546
PRIMER_RIGHT_13_TM=60.364
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=52.381
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=919
PRIMER_PAIR_14_PENALTY=1.931852
PRIMER_LEFT_14_PENALTY=1.437041
PRIMER_RIGHT_14_PENALTY=0.494811
PRIMER_LEFT_14_SEQUENCE=AATCTGCTTTTGAGGAATGGG
PRIMER_RIGHT_14_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_14=465,21
PRIMER_RIGHT_14=1386,20
PRIMER_LEFT_14_TM=60.437
PRIMER_RIGHT_14_TM=59.505
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=922
PRIMER_PAIR_15_PENALTY=1.963521
PRIMER_LEFT_15_PENALTY=1.468710
PRIMER_RIGHT_15_PENALTY=0.494811
PRIMER_LEFT_15_SEQUENCE=CTGCTTTTGAGGAATGGGTTT
PRIMER_RIGHT_15_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_15=468,21
PRIMER_RIGHT_15=1386,20
PRIMER_LEFT_15_TM=60.469
PRIMER_RIGHT_15_TM=59.505
PRIMER_LEFT_15_GC_PERCENT=42.857
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=919
PRIMER_PAIR_16_PENALTY=2.072962
PRIMER_LEFT_16_PENALTY=1.578152
PRIMER_RIGHT_16_PENALTY=0.494811
PRIMER_LEFT_16_SEQUENCE=TCAATTCCTTGCTTTGAGGA
PRIMER_RIGHT_16_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_16=504,20
PRIMER_RIGHT_16=1386,20
PRIMER_LEFT_16_TM=58.422
PRIMER_RIGHT_16_TM=59.505
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=883
PRIMER_PAIR_17_PENALTY=2.077959
PRIMER_LEFT_17_PENALTY=1.437041
PRIMER_RIGHT_17_PENALTY=0.640918
PRIMER_LEFT_17_SEQUENCE=AATCTGCTTTTGAGGAATGGG
PRIMER_RIGHT_17_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_17=465,21
PRIMER_RIGHT_17=1387,20
PRIMER_LEFT_17_TM=60.437
PRIMER_RIGHT_17_TM=59.359
PRIMER_LEFT_17_GC_PERCENT=42.857
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=923
PRIMER_PAIR_18_PENALTY=2.109628
PRIMER_LEFT_18_PENALTY=1.468710
PRIMER_RIGHT_18_PENALTY=0.640918
PRIMER_LEFT_18_SEQUENCE=CTGCTTTTGAGGAATGGGTTT
PRIMER_RIGHT_18_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_18=468,21
PRIMER_RIGHT_18=1387,20
PRIMER_LEFT_18_TM=60.469
PRIMER_RIGHT_18_TM=59.359
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=920
PRIMER_PAIR_19_PENALTY=2.194040
PRIMER_LEFT_19_PENALTY=0.829561
PRIMER_RIGHT_19_PENALTY=1.364479
PRIMER_LEFT_19_SEQUENCE=CTGCTTTTGAGGAATGGGTT
PRIMER_RIGHT_19_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_19=468,20
PRIMER_RIGHT_19=1387,21
PRIMER_LEFT_19_TM=59.170
PRIMER_RIGHT_19_TM=60.364
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=920
PRIMER_PAIR_20_PENALTY=2.219069
PRIMER_LEFT_20_PENALTY=1.578152
PRIMER_RIGHT_20_PENALTY=0.640918
PRIMER_LEFT_20_SEQUENCE=TCAATTCCTTGCTTTGAGGA
PRIMER_RIGHT_20_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_20=504,20
PRIMER_RIGHT_20=1387,20
PRIMER_LEFT_20_TM=58.422
PRIMER_RIGHT_20_TM=59.359
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.9000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=884
PRIMER_PAIR_21_PENALTY=2.219478
PRIMER_LEFT_21_PENALTY=1.724668
PRIMER_RIGHT_21_PENALTY=0.494811
PRIMER_LEFT_21_SEQUENCE=CGGACTTGCTTTCGCTTTATT
PRIMER_RIGHT_21_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_21=433,21
PRIMER_RIGHT_21=1386,20
PRIMER_LEFT_21_TM=60.725
PRIMER_RIGHT_21_TM=59.505
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.2000
PRIMER_RIGHT_21_END_STABILITY=6.6000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=954
PRIMER_PAIR_22_PENALTY=2.238979
PRIMER_LEFT_22_PENALTY=0.874500
PRIMER_RIGHT_22_PENALTY=1.364479
PRIMER_LEFT_22_SEQUENCE=ATCTGCTTTTGAGGAATGGG
PRIMER_RIGHT_22_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_22=466,20
PRIMER_RIGHT_22=1387,21
PRIMER_LEFT_22_TM=59.126
PRIMER_RIGHT_22_TM=60.364
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=922
PRIMER_PAIR_23_PENALTY=2.328188
PRIMER_LEFT_23_PENALTY=0.963709
PRIMER_RIGHT_23_PENALTY=1.364479
PRIMER_LEFT_23_SEQUENCE=TACACGGACTTGCTTTCGCT
PRIMER_RIGHT_23_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_23=429,20
PRIMER_RIGHT_23=1387,21
PRIMER_LEFT_23_TM=60.964
PRIMER_RIGHT_23_TM=60.364
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.9000
PRIMER_RIGHT_23_END_STABILITY=6.6000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=959
PRIMER_PAIR_24_PENALTY=2.333041
PRIMER_LEFT_24_PENALTY=1.838230
PRIMER_RIGHT_24_PENALTY=0.494811
PRIMER_LEFT_24_SEQUENCE=GCTTTTGAGGAATGGGTTTT
PRIMER_RIGHT_24_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_24=470,20
PRIMER_RIGHT_24=1386,20
PRIMER_LEFT_24_TM=58.162
PRIMER_RIGHT_24_TM=59.505
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=917
PRIMER_PAIR_25_PENALTY=2.365585
PRIMER_LEFT_25_PENALTY=1.724668
PRIMER_RIGHT_25_PENALTY=0.640918
PRIMER_LEFT_25_SEQUENCE=CGGACTTGCTTTCGCTTTATT
PRIMER_RIGHT_25_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_25=433,21
PRIMER_RIGHT_25=1387,20
PRIMER_LEFT_25_TM=60.725
PRIMER_RIGHT_25_TM=59.359
PRIMER_LEFT_25_GC_PERCENT=42.857
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.2000
PRIMER_RIGHT_25_END_STABILITY=6.9000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=955
PRIMER_PAIR_26_PENALTY=2.410311
PRIMER_LEFT_26_PENALTY=1.045831
PRIMER_RIGHT_26_PENALTY=1.364479
PRIMER_LEFT_26_SEQUENCE=ATCTGCTTTTGAGGAATGGGT
PRIMER_RIGHT_26_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_26=466,21
PRIMER_RIGHT_26=1387,21
PRIMER_LEFT_26_TM=59.954
PRIMER_RIGHT_26_TM=60.364
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=52.381
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.4000
PRIMER_RIGHT_26_END_STABILITY=6.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=922
PRIMER_PAIR_27_PENALTY=2.467108
PRIMER_LEFT_27_PENALTY=1.972297
PRIMER_RIGHT_27_PENALTY=0.494811
PRIMER_LEFT_27_SEQUENCE=TCTGCTTTTGAGGAATGGGTT
PRIMER_RIGHT_27_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_27=467,21
PRIMER_RIGHT_27=1386,20
PRIMER_LEFT_27_TM=60.972
PRIMER_RIGHT_27_TM=59.505
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.4000
PRIMER_RIGHT_27_END_STABILITY=6.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=920
PRIMER_PAIR_28_PENALTY=2.472201
PRIMER_LEFT_28_PENALTY=1.977390
PRIMER_RIGHT_28_PENALTY=0.494811
PRIMER_LEFT_28_SEQUENCE=GACTTGCTTTCGCTTTATTGG
PRIMER_RIGHT_28_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_28=435,21
PRIMER_RIGHT_28=1386,20
PRIMER_LEFT_28_TM=59.023
PRIMER_RIGHT_28_TM=59.505
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.4000
PRIMER_RIGHT_28_END_STABILITY=6.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=952
PRIMER_PAIR_29_PENALTY=2.472201
PRIMER_LEFT_29_PENALTY=1.977390
PRIMER_RIGHT_29_PENALTY=0.494811
PRIMER_LEFT_29_SEQUENCE=GGACTTGCTTTCGCTTTATTG
PRIMER_RIGHT_29_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_29=434,21
PRIMER_RIGHT_29=1386,20
PRIMER_LEFT_29_TM=59.023
PRIMER_RIGHT_29_TM=59.505
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.2000
PRIMER_RIGHT_29_END_STABILITY=6.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=953
PRIMER_PAIR_30_PENALTY=2.479148
PRIMER_LEFT_30_PENALTY=1.838230
PRIMER_RIGHT_30_PENALTY=0.640918
PRIMER_LEFT_30_SEQUENCE=GCTTTTGAGGAATGGGTTTT
PRIMER_RIGHT_30_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_30=470,20
PRIMER_RIGHT_30=1387,20
PRIMER_LEFT_30_TM=58.162
PRIMER_RIGHT_30_TM=59.359
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=918
PRIMER_PAIR_31_PENALTY=2.613215
PRIMER_LEFT_31_PENALTY=1.972297
PRIMER_RIGHT_31_PENALTY=0.640918
PRIMER_LEFT_31_SEQUENCE=TCTGCTTTTGAGGAATGGGTT
PRIMER_RIGHT_31_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_31=467,21
PRIMER_RIGHT_31=1387,20
PRIMER_LEFT_31_TM=60.972
PRIMER_RIGHT_31_TM=59.359
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=6.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=921
PRIMER_PAIR_32_PENALTY=2.618308
PRIMER_LEFT_32_PENALTY=1.977390
PRIMER_RIGHT_32_PENALTY=0.640918
PRIMER_LEFT_32_SEQUENCE=GACTTGCTTTCGCTTTATTGG
PRIMER_RIGHT_32_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_32=435,21
PRIMER_RIGHT_32=1387,20
PRIMER_LEFT_32_TM=59.023
PRIMER_RIGHT_32_TM=59.359
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.4000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=953
PRIMER_PAIR_33_PENALTY=2.618308
PRIMER_LEFT_33_PENALTY=1.977390
PRIMER_RIGHT_33_PENALTY=0.640918
PRIMER_LEFT_33_SEQUENCE=GGACTTGCTTTCGCTTTATTG
PRIMER_RIGHT_33_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_33=434,21
PRIMER_RIGHT_33=1387,20
PRIMER_LEFT_33_TM=59.023
PRIMER_RIGHT_33_TM=59.359
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=6.2000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=954
PRIMER_PAIR_34_PENALTY=2.747518
PRIMER_LEFT_34_PENALTY=2.252707
PRIMER_RIGHT_34_PENALTY=0.494811
PRIMER_LEFT_34_SEQUENCE=ACACGGACTTGCTTTCGCTT
PRIMER_RIGHT_34_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_34=430,20
PRIMER_RIGHT_34=1386,20
PRIMER_LEFT_34_TM=62.253
PRIMER_RIGHT_34_TM=59.505
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=10.2000
PRIMER_RIGHT_34_END_STABILITY=6.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=957
PRIMER_PAIR_35_PENALTY=2.801520
PRIMER_LEFT_35_PENALTY=1.437041
PRIMER_RIGHT_35_PENALTY=1.364479
PRIMER_LEFT_35_SEQUENCE=AATCTGCTTTTGAGGAATGGG
PRIMER_RIGHT_35_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_35=465,21
PRIMER_RIGHT_35=1387,21
PRIMER_LEFT_35_TM=60.437
PRIMER_RIGHT_35_TM=60.364
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.6000
PRIMER_RIGHT_35_END_STABILITY=6.6000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=923
PRIMER_PAIR_36_PENALTY=2.833189
PRIMER_LEFT_36_PENALTY=1.468710
PRIMER_RIGHT_36_PENALTY=1.364479
PRIMER_LEFT_36_SEQUENCE=CTGCTTTTGAGGAATGGGTTT
PRIMER_RIGHT_36_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_36=468,21
PRIMER_RIGHT_36=1387,21
PRIMER_LEFT_36_TM=60.469
PRIMER_RIGHT_36_TM=60.364
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=52.381
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=6.6000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=920
PRIMER_PAIR_37_PENALTY=2.893625
PRIMER_LEFT_37_PENALTY=2.252707
PRIMER_RIGHT_37_PENALTY=0.640918
PRIMER_LEFT_37_SEQUENCE=ACACGGACTTGCTTTCGCTT
PRIMER_RIGHT_37_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_37=430,20
PRIMER_RIGHT_37=1387,20
PRIMER_LEFT_37_TM=62.253
PRIMER_RIGHT_37_TM=59.359
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=10.2000
PRIMER_RIGHT_37_END_STABILITY=6.9000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=958
PRIMER_PAIR_38_PENALTY=2.942631
PRIMER_LEFT_38_PENALTY=1.578152
PRIMER_RIGHT_38_PENALTY=1.364479
PRIMER_LEFT_38_SEQUENCE=TCAATTCCTTGCTTTGAGGA
PRIMER_RIGHT_38_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_38=504,20
PRIMER_RIGHT_38=1387,21
PRIMER_LEFT_38_TM=58.422
PRIMER_RIGHT_38_TM=60.364
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=6.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=884
PRIMER_PAIR_39_PENALTY=3.089147
PRIMER_LEFT_39_PENALTY=1.724668
PRIMER_RIGHT_39_PENALTY=1.364479
PRIMER_LEFT_39_SEQUENCE=CGGACTTGCTTTCGCTTTATT
PRIMER_RIGHT_39_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_39=433,21
PRIMER_RIGHT_39=1387,21
PRIMER_LEFT_39_TM=60.725
PRIMER_RIGHT_39_TM=60.364
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.2000
PRIMER_RIGHT_39_END_STABILITY=6.6000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=955
PRIMER_PAIR_40_PENALTY=3.202710
PRIMER_LEFT_40_PENALTY=1.838230
PRIMER_RIGHT_40_PENALTY=1.364479
PRIMER_LEFT_40_SEQUENCE=GCTTTTGAGGAATGGGTTTT
PRIMER_RIGHT_40_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_40=470,20
PRIMER_RIGHT_40=1387,21
PRIMER_LEFT_40_TM=58.162
PRIMER_RIGHT_40_TM=60.364
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=6.6000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=918
PRIMER_PAIR_41_PENALTY=3.207554
PRIMER_LEFT_41_PENALTY=2.712744
PRIMER_RIGHT_41_PENALTY=0.494811
PRIMER_LEFT_41_SEQUENCE=CACGGACTTGCTTTCGCTTT
PRIMER_RIGHT_41_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_41=431,20
PRIMER_RIGHT_41=1386,20
PRIMER_LEFT_41_TM=62.713
PRIMER_RIGHT_41_TM=59.505
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=6.6000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=956
PRIMER_PAIR_42_PENALTY=3.269374
PRIMER_LEFT_42_PENALTY=2.774563
PRIMER_RIGHT_42_PENALTY=0.494811
PRIMER_LEFT_42_SEQUENCE=ACTTGCTTTCGCTTTATTGG
PRIMER_RIGHT_42_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_42=436,20
PRIMER_RIGHT_42=1386,20
PRIMER_LEFT_42_TM=57.225
PRIMER_RIGHT_42_TM=59.505
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=8.4000
PRIMER_RIGHT_42_END_STABILITY=6.6000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=951
PRIMER_PAIR_43_PENALTY=3.336777
PRIMER_LEFT_43_PENALTY=1.972297
PRIMER_RIGHT_43_PENALTY=1.364479
PRIMER_LEFT_43_SEQUENCE=TCTGCTTTTGAGGAATGGGTT
PRIMER_RIGHT_43_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_43=467,21
PRIMER_RIGHT_43=1387,21
PRIMER_LEFT_43_TM=60.972
PRIMER_RIGHT_43_TM=60.364
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=6.6000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=921
PRIMER_PAIR_44_PENALTY=3.341869
PRIMER_LEFT_44_PENALTY=1.977390
PRIMER_RIGHT_44_PENALTY=1.364479
PRIMER_LEFT_44_SEQUENCE=GACTTGCTTTCGCTTTATTGG
PRIMER_RIGHT_44_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_44=435,21
PRIMER_RIGHT_44=1387,21
PRIMER_LEFT_44_TM=59.023
PRIMER_RIGHT_44_TM=60.364
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.4000
PRIMER_RIGHT_44_END_STABILITY=6.6000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=953
PRIMER_PAIR_45_PENALTY=3.341869
PRIMER_LEFT_45_PENALTY=1.977390
PRIMER_RIGHT_45_PENALTY=1.364479
PRIMER_LEFT_45_SEQUENCE=GGACTTGCTTTCGCTTTATTG
PRIMER_RIGHT_45_SEQUENCE=GTCTCAAGGTGGGGACAATCT
PRIMER_LEFT_45=434,21
PRIMER_RIGHT_45=1387,21
PRIMER_LEFT_45_TM=59.023
PRIMER_RIGHT_45_TM=60.364
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.2000
PRIMER_RIGHT_45_END_STABILITY=6.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=954
PRIMER_PAIR_46_PENALTY=3.353661
PRIMER_LEFT_46_PENALTY=2.712744
PRIMER_RIGHT_46_PENALTY=0.640918
PRIMER_LEFT_46_SEQUENCE=CACGGACTTGCTTTCGCTTT
PRIMER_RIGHT_46_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_46=431,20
PRIMER_RIGHT_46=1387,20
PRIMER_LEFT_46_TM=62.713
PRIMER_RIGHT_46_TM=59.359
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=957
PRIMER_PAIR_47_PENALTY=3.415481
PRIMER_LEFT_47_PENALTY=2.774563
PRIMER_RIGHT_47_PENALTY=0.640918
PRIMER_LEFT_47_SEQUENCE=ACTTGCTTTCGCTTTATTGG
PRIMER_RIGHT_47_SEQUENCE=GTCTCAAGGTGGGGACAATC
PRIMER_LEFT_47=436,20
PRIMER_RIGHT_47=1387,20
PRIMER_LEFT_47_TM=57.225
PRIMER_RIGHT_47_TM=59.359
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.4000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=952
PRIMER_PAIR_48_PENALTY=3.591909
PRIMER_LEFT_48_PENALTY=0.329550
PRIMER_RIGHT_48_PENALTY=3.262359
PRIMER_LEFT_48_SEQUENCE=TCTGCTTTTGAGGAATGGGT
PRIMER_RIGHT_48_SEQUENCE=TGTCTCAAGGTGGGGACAATC
PRIMER_LEFT_48=467,20
PRIMER_RIGHT_48=1388,21
PRIMER_LEFT_48_TM=59.670
PRIMER_RIGHT_48_TM=62.262
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.4000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=922
PRIMER_PAIR_49_PENALTY=3.615527
PRIMER_LEFT_49_PENALTY=3.120716
PRIMER_RIGHT_49_PENALTY=0.494811
PRIMER_LEFT_49_SEQUENCE=ATCAATTCCTTGCTTTGAGG
PRIMER_RIGHT_49_SEQUENCE=TCTCAAGGTGGGGACAATCT
PRIMER_LEFT_49=503,20
PRIMER_RIGHT_49=1386,20
PRIMER_LEFT_49_TM=56.879
PRIMER_RIGHT_49_TM=59.505
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.6000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=884
=
SEQUENCE_ID=MAGIC.175_2_3116222_3116318_kn-0_edi-0_20086_105934_TRUE
SEQUENCE_TARGET=950,196
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNGAACTTGCTGCATGCATGNGTAATTCTCATTTTCGTTGCATGTGCAAGTAAAACACGTTATCNNTAATTNTCATTTTCGTTGCATGTCTAAGGAAAACANGTTACCTTGAATTGGNTTTTTAANTAAATAATNAGAAANGTTTTGTGTCTTAATNTGTGTGCAAAAGTTAAACATCAATTTTGCTGGAACNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANANAGGAAGANAGGNTAAAATCGTCAACACACATATCACTTATTCAGTAGGGGATATGGAGANNAAAACANGATATAAGNGGTNTACTNTTGANNAAAAGGNAGTTNTGAGATCATTTCTNCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_4_10302880_10303263_kn-0_po-0_28803_88658_TRUE
SEQUENCE_TARGET=950,483
SEQUENCE_TEMPLATE=CNTTATTCATTCATTGTCCTTGGNCCTTANCATATTCATCAAAGGTAGCTGAGANAAGTCAGANAATNATGATTCTTAANNTNTTNNTTTAATNAAATGTAATANCATGAGANGGCAANAATCCGTTGTAGTCTAGCTGGTCAGGATACTCGGCTCTCACCCNAGAGACCCGGGTTCGAGTCCCGGCAACGGAGNNTTTTTTATAATTNTTAACCNTAAGTCAGGGGTNNTTTCGTCATTACCTNATTTGAAGAAACCCTAGTGTCTTTNATATAATTATCACACACTACGCAGCCGACGCAATTCGTGTGCCACTCTATTTGCTCATCTCCNTCGATTTCGAACCCCTAATCTTGAGATGGTAAAGTTTCATCCTTTTGATCTATGNTTATNTTCGTTCCTCGTTTTGGGGATTTAACTTNNTATTTNCGATGTTGCAGGCATCGGAGAAGAAGCTCTCGAACCCTATGAGGGATATTAAGGTCCAGAAGCTAGTTCTTAACATCTCTGTTGGTGAGAGTGGTGATCGTCTCACTCGTGCCTCCAAGGTTCGTTCTTTCTTTCCCCNTAAGAGTACTCACTATATGGCTTNGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGNTTGGTTAGTNACNTAGTTCATAGNTGNNNNNNNNNNNNNNNNNTTGANTCGAGAGTTTTGGTTATTGTAGGTGTTGGAACAGCTCAGTGGTCANACNCCTGTCTTCTCTAAGGGTNTGTGAATATNTTNATGNNNNNNNNNTTANATGTTNTAGCTATGANTTAAGGATTGTAGAGNTCAATTTTAGTGTNNTCNTGANTGTNAACAGCGAGGTACACTGTGAGGTCTTTCGGTATCAGGCGTAATGAAAAGATTGCGTGNTATGTCACNGTNAGAGGTGAGAAGGCAATGCAGCTTCTTGAGAGTGGNTTGAAAGTGAAGGAATACGAGCTGTTGAGGAGGAACTTCAGTGACACNGGCTGCTTNGGATTCGGTATCCAGGAGCACATTGATCTTGGAATCAAGTAAGTGTNGTGATAATAATATGGGCTGATTTATTANATTAAATGNTTNCTGAGGNTAATGTTCTCANTGTTCTGCATTTCTTTNTTAGGTANGATCCTTCTACCGGTATCTACGGTATGGATTTCTNCGTTGTNCTTGAACGTCCAGGATACCGTGTGGCCCGTCGCCGTAGATGCAAGACTCGCGTNGGTATNCAACATAGAGTTACCAAGGATGATGCCATGAAGTGGTTCCAAGTTAAGTATGAAGGAGTTATCCTCAACAAGTCTCAGAACATCACTGGTTGAAGAGNNNTTTTNNNTCATTTTGTTTTGTTTCAAGTTCTTATATTTAAGATTCTGGANAGTAAGGATTTCGTGTTGTGGTAACCTTTTTGACTTAAGTTGGTTAAGAATCTTCAAGTACCTTCTAAATATNAAACCATTTTTGGGGGTTTTTGTTTACNAATTCTAAACCTTTTCAGAATTTAGTTTGACCACAGGCAAGANTTGGAGATAACCAAACTTTTACAAGGTTGAGANACTTGAAAATGTTTCAGCTTTACAAGAATGATTGTCTTAATATAACAACATACAATCTGTCATCTTGTTTGCATGTAAGAGNATGTTGACTNTAAGTTACAATCAGGACGATCTTATCAGCTCCAATAGTTCACTCTTCCTCAACTTTGAGTATCCTTTGATTCCTCTGTTCTTTGCAACTTCTTTGAGCTCTGCAAGTTTCATAGTTTCTATCAGTGAAGCTTTCTCTTCCTTCTTTGTCAATTNTCCTAAAGCTTTATCGCTGTTTGATACTGGGAGCTTACCTCTTGGACCTGAGGCAGAAGAAGATAAAGGAGTTCTCTTGACAAAACTAGACGGTGGTCGAGGAAGCTCTACTTGTCTCTTTGGTGGCTTCTCCTTCACACTAGTATCTCCTACAGTTGCANAGTTGATTATATTAACAAATGGCGAGAACATGGANNTTTGACATTTGACCAAAGCATAGTTGATTACCCTCAGTTTTTTTTCTTGATTTCTCAAGAACGTCAAGAATAGCTTTGGTCAGCGGCTTTTCNTTAGAAGACTCATCGCTGTTCTTCTCTTCCTCGACTCCTCGAGACTCTCCTTTAGAGATCGAAGATTGAATCCGTTTCAAGAGCGAAATGATCTCCTCTTGGTTCGATTTACTTGGGTTTGACGTTCGTNTATCAGACGGTTTCTTGCAACAAACACATCCTTTCTTGGATCTTCCCATTGAAGNANAAGAAGAACCACCTTTCTCTTGAAACCAAATACTTCTATCTCCATTAATCGTTGTTCTCNAAAGACTCTGCTCAAAGCGCAACGCAAACGGCAGTGAAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.864558
PRIMER_LEFT_0_PENALTY=0.081493
PRIMER_RIGHT_0_PENALTY=0.783065
PRIMER_LEFT_0_SEQUENCE=GGTTCGTTCTTTCTTTCCCC
PRIMER_RIGHT_0_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_0=547,20
PRIMER_RIGHT_0=1478,20
PRIMER_LEFT_0_TM=59.919
PRIMER_RIGHT_0_TM=60.783
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=10.9000
PRIMER_RIGHT_0_END_STABILITY=7.8000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=932
PRIMER_PAIR_1_PENALTY=1.295939
PRIMER_LEFT_1_PENALTY=0.512874
PRIMER_RIGHT_1_PENALTY=0.783065
PRIMER_LEFT_1_SEQUENCE=CAGCGAGGTACACTGTGAGG
PRIMER_RIGHT_1_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_1=840,20
PRIMER_RIGHT_1=1478,20
PRIMER_LEFT_1_TM=59.487
PRIMER_RIGHT_1_TM=60.783
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=639
PRIMER_PAIR_2_PENALTY=1.438903
PRIMER_LEFT_2_PENALTY=0.863548
PRIMER_RIGHT_2_PENALTY=0.575355
PRIMER_LEFT_2_SEQUENCE=TCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_2_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_2=864,20
PRIMER_RIGHT_2=1852,20
PRIMER_LEFT_2_TM=59.136
PRIMER_RIGHT_2_TM=59.425
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=989
PRIMER_PAIR_3_PENALTY=1.473150
PRIMER_LEFT_3_PENALTY=0.690085
PRIMER_RIGHT_3_PENALTY=0.783065
PRIMER_LEFT_3_SEQUENCE=GTGTTGGAACAGCTCAGTGG
PRIMER_RIGHT_3_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_3=705,20
PRIMER_RIGHT_3=1478,20
PRIMER_LEFT_3_TM=59.310
PRIMER_RIGHT_3_TM=60.783
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=774
PRIMER_PAIR_4_PENALTY=1.500944
PRIMER_LEFT_4_PENALTY=1.222097
PRIMER_RIGHT_4_PENALTY=0.278847
PRIMER_LEFT_4_SEQUENCE=GCAATGCAGCTTCTTGAGAGT
PRIMER_RIGHT_4_SEQUENCE=TCCTCGACCACCGTCTAGTT
PRIMER_LEFT_4=920,21
PRIMER_RIGHT_4=1909,20
PRIMER_LEFT_4_TM=59.778
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=990
PRIMER_PAIR_5_PENALTY=1.579674
PRIMER_LEFT_5_PENALTY=0.863548
PRIMER_RIGHT_5_PENALTY=0.716126
PRIMER_LEFT_5_SEQUENCE=TCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_5_SEQUENCE=GTCCAAGAGGTAAGCTCCCA
PRIMER_LEFT_5=864,20
PRIMER_RIGHT_5=1853,20
PRIMER_LEFT_5_TM=59.136
PRIMER_RIGHT_5_TM=59.284
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=990
PRIMER_PAIR_6_PENALTY=1.579674
PRIMER_LEFT_6_PENALTY=0.863548
PRIMER_RIGHT_6_PENALTY=0.716126
PRIMER_LEFT_6_SEQUENCE=TCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_6_SEQUENCE=GCCTCAGGTCCAAGAGGTAA
PRIMER_LEFT_6=864,20
PRIMER_RIGHT_6=1860,20
PRIMER_LEFT_6_TM=59.136
PRIMER_RIGHT_6_TM=59.284
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=997
PRIMER_PAIR_7_PENALTY=1.603857
PRIMER_LEFT_7_PENALTY=1.028502
PRIMER_RIGHT_7_PENALTY=0.575355
PRIMER_LEFT_7_SEQUENCE=CGGTATCAGGCGTAATGAAAA
PRIMER_RIGHT_7_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_7=865,21
PRIMER_RIGHT_7=1852,20
PRIMER_LEFT_7_TM=59.971
PRIMER_RIGHT_7_TM=59.425
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=988
PRIMER_PAIR_8_PENALTY=1.646613
PRIMER_LEFT_8_PENALTY=0.863548
PRIMER_RIGHT_8_PENALTY=0.783065
PRIMER_LEFT_8_SEQUENCE=TCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_8_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_8=864,20
PRIMER_RIGHT_8=1478,20
PRIMER_LEFT_8_TM=59.136
PRIMER_RIGHT_8_TM=60.783
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.8000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=615
PRIMER_PAIR_9_PENALTY=1.648901
PRIMER_LEFT_9_PENALTY=1.370054
PRIMER_RIGHT_9_PENALTY=0.278847
PRIMER_LEFT_9_SEQUENCE=AATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_9_SEQUENCE=TCCTCGACCACCGTCTAGTT
PRIMER_LEFT_9=922,21
PRIMER_RIGHT_9=1909,20
PRIMER_LEFT_9_TM=59.630
PRIMER_RIGHT_9_TM=59.721
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=5.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=988
PRIMER_PAIR_10_PENALTY=1.681877
PRIMER_LEFT_10_PENALTY=0.898812
PRIMER_RIGHT_10_PENALTY=0.783065
PRIMER_LEFT_10_SEQUENCE=TTGGTGAGAGTGGTGATCGT
PRIMER_RIGHT_10_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_10=510,20
PRIMER_RIGHT_10=1478,20
PRIMER_LEFT_10_TM=59.101
PRIMER_RIGHT_10_TM=60.783
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.0000
PRIMER_RIGHT_10_END_STABILITY=7.8000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=969
PRIMER_PAIR_11_PENALTY=1.744628
PRIMER_LEFT_11_PENALTY=1.028502
PRIMER_RIGHT_11_PENALTY=0.716126
PRIMER_LEFT_11_SEQUENCE=CGGTATCAGGCGTAATGAAAA
PRIMER_RIGHT_11_SEQUENCE=GTCCAAGAGGTAAGCTCCCA
PRIMER_LEFT_11=865,21
PRIMER_RIGHT_11=1853,20
PRIMER_LEFT_11_TM=59.971
PRIMER_RIGHT_11_TM=59.284
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=989
PRIMER_PAIR_12_PENALTY=1.744628
PRIMER_LEFT_12_PENALTY=1.028502
PRIMER_RIGHT_12_PENALTY=0.716126
PRIMER_LEFT_12_SEQUENCE=CGGTATCAGGCGTAATGAAAA
PRIMER_RIGHT_12_SEQUENCE=GCCTCAGGTCCAAGAGGTAA
PRIMER_LEFT_12=865,21
PRIMER_RIGHT_12=1860,20
PRIMER_LEFT_12_TM=59.971
PRIMER_RIGHT_12_TM=59.284
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=996
PRIMER_PAIR_13_PENALTY=1.745135
PRIMER_LEFT_13_PENALTY=1.222097
PRIMER_RIGHT_13_PENALTY=0.523038
PRIMER_LEFT_13_SEQUENCE=GCAATGCAGCTTCTTGAGAGT
PRIMER_RIGHT_13_SEQUENCE=CTTCTTCTGCCTCAGGTCCA
PRIMER_LEFT_13=920,21
PRIMER_RIGHT_13=1868,20
PRIMER_LEFT_13_TM=59.778
PRIMER_RIGHT_13_TM=60.523
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=949
PRIMER_PAIR_14_PENALTY=1.772461
PRIMER_LEFT_14_PENALTY=0.989397
PRIMER_RIGHT_14_PENALTY=0.783065
PRIMER_LEFT_14_SEQUENCE=GATCGTCTCACTCGTGCCTC
PRIMER_RIGHT_14_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_14=524,20
PRIMER_RIGHT_14=1478,20
PRIMER_LEFT_14_TM=60.989
PRIMER_RIGHT_14_TM=60.783
PRIMER_LEFT_14_GC_PERCENT=60.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=7.8000
PRIMER_PAIR_14_COMPL_ANY=2.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=955
PRIMER_PAIR_15_PENALTY=1.791838
PRIMER_LEFT_15_PENALTY=1.008773
PRIMER_RIGHT_15_PENALTY=0.783065
PRIMER_LEFT_15_SEQUENCE=GTCTCACTCGTGCCTCCAAG
PRIMER_RIGHT_15_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_15=528,20
PRIMER_RIGHT_15=1478,20
PRIMER_LEFT_15_TM=61.009
PRIMER_RIGHT_15_TM=60.783
PRIMER_LEFT_15_GC_PERCENT=60.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=7.8000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=951
PRIMER_PAIR_16_PENALTY=1.797452
PRIMER_LEFT_16_PENALTY=1.222097
PRIMER_RIGHT_16_PENALTY=0.575355
PRIMER_LEFT_16_SEQUENCE=GCAATGCAGCTTCTTGAGAGT
PRIMER_RIGHT_16_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_16=920,21
PRIMER_RIGHT_16=1852,20
PRIMER_LEFT_16_TM=59.778
PRIMER_RIGHT_16_TM=59.425
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.1000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=933
PRIMER_PAIR_17_PENALTY=1.808234
PRIMER_LEFT_17_PENALTY=1.025170
PRIMER_RIGHT_17_PENALTY=0.783065
PRIMER_LEFT_17_SEQUENCE=GTTGGTGAGAGTGGTGATCGT
PRIMER_RIGHT_17_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_17=509,21
PRIMER_RIGHT_17=1478,20
PRIMER_LEFT_17_TM=60.025
PRIMER_RIGHT_17_TM=60.783
PRIMER_LEFT_17_GC_PERCENT=52.381
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.0000
PRIMER_RIGHT_17_END_STABILITY=7.8000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=970
PRIMER_PAIR_18_PENALTY=1.811566
PRIMER_LEFT_18_PENALTY=1.028502
PRIMER_RIGHT_18_PENALTY=0.783065
PRIMER_LEFT_18_SEQUENCE=CGGTATCAGGCGTAATGAAAA
PRIMER_RIGHT_18_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_18=865,21
PRIMER_RIGHT_18=1478,20
PRIMER_LEFT_18_TM=59.971
PRIMER_RIGHT_18_TM=60.783
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=7.8000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=614
PRIMER_PAIR_19_PENALTY=1.893092
PRIMER_LEFT_19_PENALTY=1.370054
PRIMER_RIGHT_19_PENALTY=0.523038
PRIMER_LEFT_19_SEQUENCE=AATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_19_SEQUENCE=CTTCTTCTGCCTCAGGTCCA
PRIMER_LEFT_19=922,21
PRIMER_RIGHT_19=1868,20
PRIMER_LEFT_19_TM=59.630
PRIMER_RIGHT_19_TM=60.523
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=947
PRIMER_PAIR_20_PENALTY=1.924605
PRIMER_LEFT_20_PENALTY=1.349250
PRIMER_RIGHT_20_PENALTY=0.575355
PRIMER_LEFT_20_SEQUENCE=CGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_20_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_20=865,20
PRIMER_RIGHT_20=1852,20
PRIMER_LEFT_20_TM=58.651
PRIMER_RIGHT_20_TM=59.425
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.3000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=988
PRIMER_PAIR_21_PENALTY=1.938223
PRIMER_LEFT_21_PENALTY=1.222097
PRIMER_RIGHT_21_PENALTY=0.716126
PRIMER_LEFT_21_SEQUENCE=GCAATGCAGCTTCTTGAGAGT
PRIMER_RIGHT_21_SEQUENCE=GTCCAAGAGGTAAGCTCCCA
PRIMER_LEFT_21=920,21
PRIMER_RIGHT_21=1853,20
PRIMER_LEFT_21_TM=59.778
PRIMER_RIGHT_21_TM=59.284
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=5.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.1000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=934
PRIMER_PAIR_22_PENALTY=1.938223
PRIMER_LEFT_22_PENALTY=1.222097
PRIMER_RIGHT_22_PENALTY=0.716126
PRIMER_LEFT_22_SEQUENCE=GCAATGCAGCTTCTTGAGAGT
PRIMER_RIGHT_22_SEQUENCE=GCCTCAGGTCCAAGAGGTAA
PRIMER_LEFT_22=920,21
PRIMER_RIGHT_22=1860,20
PRIMER_LEFT_22_TM=59.778
PRIMER_RIGHT_22_TM=59.284
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.1000
PRIMER_RIGHT_22_END_STABILITY=7.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=941
PRIMER_PAIR_23_PENALTY=1.945409
PRIMER_LEFT_23_PENALTY=1.370054
PRIMER_RIGHT_23_PENALTY=0.575355
PRIMER_LEFT_23_SEQUENCE=AATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_23_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_23=922,21
PRIMER_RIGHT_23=1852,20
PRIMER_LEFT_23_TM=59.630
PRIMER_RIGHT_23_TM=59.425
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=931
PRIMER_PAIR_24_PENALTY=2.014333
PRIMER_LEFT_24_PENALTY=1.222097
PRIMER_RIGHT_24_PENALTY=0.792236
PRIMER_LEFT_24_SEQUENCE=GCAATGCAGCTTCTTGAGAGT
PRIMER_RIGHT_24_SEQUENCE=CCTCGACCACCGTCTAGTTT
PRIMER_LEFT_24=920,21
PRIMER_RIGHT_24=1908,20
PRIMER_LEFT_24_TM=59.778
PRIMER_RIGHT_24_TM=59.208
PRIMER_LEFT_24_GC_PERCENT=47.619
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.1000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=989
PRIMER_PAIR_25_PENALTY=2.018946
PRIMER_LEFT_25_PENALTY=1.235881
PRIMER_RIGHT_25_PENALTY=0.783065
PRIMER_LEFT_25_SEQUENCE=CCTCCAAGGTTCGTTCTTTC
PRIMER_RIGHT_25_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_25=540,20
PRIMER_RIGHT_25=1478,20
PRIMER_LEFT_25_TM=58.764
PRIMER_RIGHT_25_TM=60.783
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=7.8000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=939
PRIMER_PAIR_26_PENALTY=2.037386
PRIMER_LEFT_26_PENALTY=1.462030
PRIMER_RIGHT_26_PENALTY=0.575355
PRIMER_LEFT_26_SEQUENCE=TCGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_26_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_26=864,21
PRIMER_RIGHT_26=1852,20
PRIMER_LEFT_26_TM=60.462
PRIMER_RIGHT_26_TM=59.425
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=7.3000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=989
PRIMER_PAIR_27_PENALTY=2.037386
PRIMER_LEFT_27_PENALTY=1.462030
PRIMER_RIGHT_27_PENALTY=0.575355
PRIMER_LEFT_27_SEQUENCE=TTCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_27_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_27=863,21
PRIMER_RIGHT_27=1852,20
PRIMER_LEFT_27_TM=60.462
PRIMER_RIGHT_27_TM=59.425
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=990
PRIMER_PAIR_28_PENALTY=2.045710
PRIMER_LEFT_28_PENALTY=1.262646
PRIMER_RIGHT_28_PENALTY=0.783065
PRIMER_LEFT_28_SEQUENCE=CCTCCAAGGTTCGTTCTTTCT
PRIMER_RIGHT_28_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_28=540,21
PRIMER_RIGHT_28=1478,20
PRIMER_LEFT_28_TM=59.737
PRIMER_RIGHT_28_TM=60.783
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=7.8000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=939
PRIMER_PAIR_29_PENALTY=2.065376
PRIMER_LEFT_29_PENALTY=1.349250
PRIMER_RIGHT_29_PENALTY=0.716126
PRIMER_LEFT_29_SEQUENCE=CGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_29_SEQUENCE=GTCCAAGAGGTAAGCTCCCA
PRIMER_LEFT_29=865,20
PRIMER_RIGHT_29=1853,20
PRIMER_LEFT_29_TM=58.651
PRIMER_RIGHT_29_TM=59.284
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=5.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.3000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=989
PRIMER_PAIR_30_PENALTY=2.065376
PRIMER_LEFT_30_PENALTY=1.349250
PRIMER_RIGHT_30_PENALTY=0.716126
PRIMER_LEFT_30_SEQUENCE=CGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_30_SEQUENCE=GCCTCAGGTCCAAGAGGTAA
PRIMER_LEFT_30=865,20
PRIMER_RIGHT_30=1860,20
PRIMER_LEFT_30_TM=58.651
PRIMER_RIGHT_30_TM=59.284
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.3000
PRIMER_RIGHT_30_END_STABILITY=7.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=996
PRIMER_PAIR_31_PENALTY=2.086180
PRIMER_LEFT_31_PENALTY=1.370054
PRIMER_RIGHT_31_PENALTY=0.716126
PRIMER_LEFT_31_SEQUENCE=AATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_31_SEQUENCE=GTCCAAGAGGTAAGCTCCCA
PRIMER_LEFT_31=922,21
PRIMER_RIGHT_31=1853,20
PRIMER_LEFT_31_TM=59.630
PRIMER_RIGHT_31_TM=59.284
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.9000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=932
PRIMER_PAIR_32_PENALTY=2.086180
PRIMER_LEFT_32_PENALTY=1.370054
PRIMER_RIGHT_32_PENALTY=0.716126
PRIMER_LEFT_32_SEQUENCE=AATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_32_SEQUENCE=GCCTCAGGTCCAAGAGGTAA
PRIMER_LEFT_32=922,21
PRIMER_RIGHT_32=1860,20
PRIMER_LEFT_32_TM=59.630
PRIMER_RIGHT_32_TM=59.284
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.9000
PRIMER_RIGHT_32_END_STABILITY=7.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=939
PRIMER_PAIR_33_PENALTY=2.092051
PRIMER_LEFT_33_PENALTY=1.813204
PRIMER_RIGHT_33_PENALTY=0.278847
PRIMER_LEFT_33_SEQUENCE=ATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_33_SEQUENCE=TCCTCGACCACCGTCTAGTT
PRIMER_LEFT_33=923,20
PRIMER_RIGHT_33=1909,20
PRIMER_LEFT_33_TM=58.187
PRIMER_RIGHT_33_TM=59.721
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=5.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=987
PRIMER_PAIR_34_PENALTY=2.116962
PRIMER_LEFT_34_PENALTY=0.863548
PRIMER_RIGHT_34_PENALTY=1.253414
PRIMER_LEFT_34_SEQUENCE=TCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_34_SEQUENCE=TCCAAGAGGTAAGCTCCCAGT
PRIMER_LEFT_34=864,20
PRIMER_RIGHT_34=1852,21
PRIMER_LEFT_34_TM=59.136
PRIMER_RIGHT_34_TM=60.253
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=52.381
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=6.9000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=989
PRIMER_PAIR_35_PENALTY=2.116962
PRIMER_LEFT_35_PENALTY=0.863548
PRIMER_RIGHT_35_PENALTY=1.253414
PRIMER_LEFT_35_SEQUENCE=TCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_35_SEQUENCE=GTCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_35=864,20
PRIMER_RIGHT_35=1853,21
PRIMER_LEFT_35_TM=59.136
PRIMER_RIGHT_35_TM=60.253
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=57.143
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.9000
PRIMER_RIGHT_35_END_STABILITY=9.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=990
PRIMER_PAIR_36_PENALTY=2.116962
PRIMER_LEFT_36_PENALTY=0.863548
PRIMER_RIGHT_36_PENALTY=1.253414
PRIMER_LEFT_36_SEQUENCE=TCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_36_SEQUENCE=GCCTCAGGTCCAAGAGGTAAG
PRIMER_LEFT_36=864,20
PRIMER_RIGHT_36=1860,21
PRIMER_LEFT_36_TM=59.136
PRIMER_RIGHT_36_TM=60.253
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=57.143
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=5.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=5.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=997
PRIMER_PAIR_37_PENALTY=2.132315
PRIMER_LEFT_37_PENALTY=1.349250
PRIMER_RIGHT_37_PENALTY=0.783065
PRIMER_LEFT_37_SEQUENCE=CGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_37_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_37=865,20
PRIMER_RIGHT_37=1478,20
PRIMER_LEFT_37_TM=58.651
PRIMER_RIGHT_37_TM=60.783
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.3000
PRIMER_RIGHT_37_END_STABILITY=7.8000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=614
PRIMER_PAIR_38_PENALTY=2.153118
PRIMER_LEFT_38_PENALTY=1.370054
PRIMER_RIGHT_38_PENALTY=0.783065
PRIMER_LEFT_38_SEQUENCE=AATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_38_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_38=922,21
PRIMER_RIGHT_38=1478,20
PRIMER_LEFT_38_TM=59.630
PRIMER_RIGHT_38_TM=60.783
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=7.8000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=557
PRIMER_PAIR_39_PENALTY=2.155603
PRIMER_LEFT_39_PENALTY=1.372538
PRIMER_RIGHT_39_PENALTY=0.783065
PRIMER_LEFT_39_SEQUENCE=CAGCGAGGTACACTGTGAGGT
PRIMER_RIGHT_39_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_39=840,21
PRIMER_RIGHT_39=1478,20
PRIMER_LEFT_39_TM=60.373
PRIMER_RIGHT_39_TM=60.783
PRIMER_LEFT_39_GC_PERCENT=57.143
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.6000
PRIMER_RIGHT_39_END_STABILITY=7.8000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=639
PRIMER_PAIR_40_PENALTY=2.155603
PRIMER_LEFT_40_PENALTY=1.372538
PRIMER_RIGHT_40_PENALTY=0.783065
PRIMER_LEFT_40_SEQUENCE=ACAGCGAGGTACACTGTGAGG
PRIMER_RIGHT_40_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_40=839,21
PRIMER_RIGHT_40=1478,20
PRIMER_LEFT_40_TM=60.373
PRIMER_RIGHT_40_TM=60.783
PRIMER_LEFT_40_GC_PERCENT=57.143
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=7.8000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=640
PRIMER_PAIR_41_PENALTY=2.156507
PRIMER_LEFT_41_PENALTY=1.633469
PRIMER_RIGHT_41_PENALTY=0.523038
PRIMER_LEFT_41_SEQUENCE=GCGTAATGAAAAGATTGCGTG
PRIMER_RIGHT_41_SEQUENCE=CTTCTTCTGCCTCAGGTCCA
PRIMER_LEFT_41=874,21
PRIMER_RIGHT_41=1868,20
PRIMER_LEFT_41_TM=60.633
PRIMER_RIGHT_41_TM=60.523
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=995
PRIMER_PAIR_42_PENALTY=2.162290
PRIMER_LEFT_42_PENALTY=1.370054
PRIMER_RIGHT_42_PENALTY=0.792236
PRIMER_LEFT_42_SEQUENCE=AATGCAGCTTCTTGAGAGTGG
PRIMER_RIGHT_42_SEQUENCE=CCTCGACCACCGTCTAGTTT
PRIMER_LEFT_42=922,21
PRIMER_RIGHT_42=1908,20
PRIMER_LEFT_42_TM=59.630
PRIMER_RIGHT_42_TM=59.208
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=987
PRIMER_PAIR_43_PENALTY=2.178156
PRIMER_LEFT_43_PENALTY=1.462030
PRIMER_RIGHT_43_PENALTY=0.716126
PRIMER_LEFT_43_SEQUENCE=TCGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_43_SEQUENCE=GTCCAAGAGGTAAGCTCCCA
PRIMER_LEFT_43=864,21
PRIMER_RIGHT_43=1853,20
PRIMER_LEFT_43_TM=60.462
PRIMER_RIGHT_43_TM=59.284
PRIMER_LEFT_43_GC_PERCENT=42.857
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=990
PRIMER_PAIR_44_PENALTY=2.178156
PRIMER_LEFT_44_PENALTY=1.462030
PRIMER_RIGHT_44_PENALTY=0.716126
PRIMER_LEFT_44_SEQUENCE=TCGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_44_SEQUENCE=GCCTCAGGTCCAAGAGGTAA
PRIMER_LEFT_44=864,21
PRIMER_RIGHT_44=1860,20
PRIMER_LEFT_44_TM=60.462
PRIMER_RIGHT_44_TM=59.284
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=7.2000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=997
PRIMER_PAIR_45_PENALTY=2.178156
PRIMER_LEFT_45_PENALTY=1.462030
PRIMER_RIGHT_45_PENALTY=0.716126
PRIMER_LEFT_45_SEQUENCE=TTCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_45_SEQUENCE=GTCCAAGAGGTAAGCTCCCA
PRIMER_LEFT_45=863,21
PRIMER_RIGHT_45=1853,20
PRIMER_LEFT_45_TM=60.462
PRIMER_RIGHT_45_TM=59.284
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.9000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=991
PRIMER_PAIR_46_PENALTY=2.178156
PRIMER_LEFT_46_PENALTY=1.462030
PRIMER_RIGHT_46_PENALTY=0.716126
PRIMER_LEFT_46_SEQUENCE=TTCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_46_SEQUENCE=GCCTCAGGTCCAAGAGGTAA
PRIMER_LEFT_46=863,21
PRIMER_RIGHT_46=1860,20
PRIMER_LEFT_46_TM=60.462
PRIMER_RIGHT_46_TM=59.284
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=5.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.9000
PRIMER_RIGHT_46_END_STABILITY=7.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=998
PRIMER_PAIR_47_PENALTY=2.208825
PRIMER_LEFT_47_PENALTY=1.633469
PRIMER_RIGHT_47_PENALTY=0.575355
PRIMER_LEFT_47_SEQUENCE=GCGTAATGAAAAGATTGCGTG
PRIMER_RIGHT_47_SEQUENCE=TCCAAGAGGTAAGCTCCCAG
PRIMER_LEFT_47=874,21
PRIMER_RIGHT_47=1852,20
PRIMER_LEFT_47_TM=60.633
PRIMER_RIGHT_47_TM=59.425
PRIMER_LEFT_47_GC_PERCENT=42.857
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=979
PRIMER_PAIR_48_PENALTY=2.245095
PRIMER_LEFT_48_PENALTY=1.462030
PRIMER_RIGHT_48_PENALTY=0.783065
PRIMER_LEFT_48_SEQUENCE=TCGGTATCAGGCGTAATGAAA
PRIMER_RIGHT_48_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_48=864,21
PRIMER_RIGHT_48=1478,20
PRIMER_LEFT_48_TM=60.462
PRIMER_RIGHT_48_TM=60.783
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.3000
PRIMER_RIGHT_48_END_STABILITY=7.8000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=615
PRIMER_PAIR_49_PENALTY=2.245095
PRIMER_LEFT_49_PENALTY=1.462030
PRIMER_RIGHT_49_PENALTY=0.783065
PRIMER_LEFT_49_SEQUENCE=TTCGGTATCAGGCGTAATGAA
PRIMER_RIGHT_49_SEQUENCE=CAAAAACCCCCAAAAATGGT
PRIMER_LEFT_49=863,21
PRIMER_RIGHT_49=1478,20
PRIMER_LEFT_49_TM=60.462
PRIMER_RIGHT_49_TM=60.783
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=7.8000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=616
=
SEQUENCE_ID=MAGIC.446_3_6265600_6265784_wu-0_po-0_35493_132627_TRUE
SEQUENCE_TARGET=950,284
SEQUENCE_TEMPLATE=NNNTTTTATGGTCATNTGANTTTTGAAAAGAATCCAAAATAGAATACAAAGCNCAACCAAAAGAATTTAAGCCNNNAAAAAAAAAATACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATGCAAGAAAAANATAAACANTAGAATTATAAAGCAGAACTNTTAAAGCCAAAATCNAAAGATATCGAAATCATATACTAAGANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANTAAAATGTCAATNTNNACAGACAAATACAAANGTTTATTCAAATTGTATTGGTAAAAGCTAATTTTGAGAATCGTTTTNNNNNAACAACCAGCACAATCAACAATTTTNTNTANNAAAAAATCTNTAATATGAAAATTCAAAAACATGCCGTAAGATAATTATTAGTGNNTTTGTTTAAGATAAAGTTATGTACGTTACACTTTATTTATATACAAATTATATGACATNTATAATGCACNNTAGNANGAGCTTTTTAATNAAANATTTACACAATATATTTTATTACATATAAGAAGAGCAATAATAACAATCGAAATTGTGACATACTAGGCGTGAGAATCTTAAAGTTTATNTCACGATAGATNATGTTCTGGACCCACTTTCTCTTATTTCATTCNATCATTNACNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAACAAAANTACTAATACTNTTTTGTAAGCCTTGNNNNTTTCTCTGTGNTAAAGTTATATAGTTTCGTTGTTACAANGATGTCATCTCCATTTTCTAGTTTTTGANAAGAGTANAAGTATATCAACCCTCAAAGTNGAGAACACATGTTTGCATGAGCTNCAAAAACAACAACCAACCAANNTGTGTGAAGTGTGAACANNTTTGTNGTGANNTACGCACCAAACANTCCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTTCATTAATTTACTACAATTGTGTCAAAAGAAGAATGTTCAATGAGTGATTGTGGAAATGATTATTCACACGTATCCCAAAACGCACGCTTCATCTCAAGATCATCATTTTGTAAANAAATATTAATTGATTTGNTGTTGTTNTGTGTGTGTGAGATTCATTGAAGTAATAATGACGATAAAGGAGACGGCAACAAATATTGGCACGACACAAGTACCATCACCGAAAGTCCTCGGCGCCTCTTTCGCCTCCTAAGTTTTCCTACTTCGACCCCACTTTGTTTTTCGTTTGTTAAATTCCTCTTATTTTTGTGAATCCATATATANAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTANCCATATGATNTTCATCAAGTCAAAGTCACGAGAGTTCTTAATATGCATCCACATTGTTAATATTCCAATGCAATTATTTTAGTTATAAGCAATATTCAAATTGATTTAAATTTGGATGGCATGATACTATACGAATGCATGATAAAAAAGCATCGAGCTCTTTATTAACAACTACACCAAAGGTCATCC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.462895
PRIMER_LEFT_0_PENALTY=2.429424
PRIMER_RIGHT_0_PENALTY=0.033470
PRIMER_LEFT_0_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_0_SEQUENCE=GCCAATATTTGTTGCCGTCT
PRIMER_LEFT_0=718,20
PRIMER_RIGHT_0=1559,20
PRIMER_LEFT_0_TM=57.571
PRIMER_RIGHT_0_TM=59.967
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=8.1000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=842
PRIMER_PAIR_1_PENALTY=2.642930
PRIMER_LEFT_1_PENALTY=2.429424
PRIMER_RIGHT_1_PENALTY=0.213506
PRIMER_LEFT_1_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_1_SEQUENCE=TTGCCGTCTCCTTTATCGTC
PRIMER_LEFT_1=719,20
PRIMER_RIGHT_1=1548,20
PRIMER_LEFT_1_TM=57.571
PRIMER_RIGHT_1_TM=60.214
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=830
PRIMER_PAIR_2_PENALTY=2.642930
PRIMER_LEFT_2_PENALTY=2.429424
PRIMER_RIGHT_2_PENALTY=0.213506
PRIMER_LEFT_2_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_2_SEQUENCE=TTGCCGTCTCCTTTATCGTC
PRIMER_LEFT_2=718,20
PRIMER_RIGHT_2=1548,20
PRIMER_LEFT_2_TM=57.571
PRIMER_RIGHT_2_TM=60.214
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=831
PRIMER_PAIR_3_PENALTY=2.842802
PRIMER_LEFT_3_PENALTY=2.429424
PRIMER_RIGHT_3_PENALTY=0.413377
PRIMER_LEFT_3_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_3_SEQUENCE=CAAAGTGGGGTCGAAGTAGG
PRIMER_LEFT_3=719,20
PRIMER_RIGHT_3=1635,20
PRIMER_LEFT_3_TM=57.571
PRIMER_RIGHT_3_TM=59.587
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=917
PRIMER_PAIR_4_PENALTY=2.842802
PRIMER_LEFT_4_PENALTY=2.429424
PRIMER_RIGHT_4_PENALTY=0.413377
PRIMER_LEFT_4_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_4_SEQUENCE=CAAAGTGGGGTCGAAGTAGG
PRIMER_LEFT_4=718,20
PRIMER_RIGHT_4=1635,20
PRIMER_LEFT_4_TM=57.571
PRIMER_RIGHT_4_TM=59.587
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=918
PRIMER_PAIR_5_PENALTY=2.990098
PRIMER_LEFT_5_PENALTY=2.429424
PRIMER_RIGHT_5_PENALTY=0.560673
PRIMER_LEFT_5_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_5_SEQUENCE=CGTTTTGGGATACGTGTGAA
PRIMER_LEFT_5=719,20
PRIMER_RIGHT_5=1439,20
PRIMER_LEFT_5_TM=57.571
PRIMER_RIGHT_5_TM=59.439
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=721
PRIMER_PAIR_6_PENALTY=2.990098
PRIMER_LEFT_6_PENALTY=2.429424
PRIMER_RIGHT_6_PENALTY=0.560673
PRIMER_LEFT_6_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_6_SEQUENCE=CGTTTTGGGATACGTGTGAA
PRIMER_LEFT_6=718,20
PRIMER_RIGHT_6=1439,20
PRIMER_LEFT_6_TM=57.571
PRIMER_RIGHT_6_TM=59.439
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=722
PRIMER_PAIR_7_PENALTY=3.191194
PRIMER_LEFT_7_PENALTY=2.429424
PRIMER_RIGHT_7_PENALTY=0.761770
PRIMER_LEFT_7_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_7_SEQUENCE=GTGCCAATATTTGTTGCCGT
PRIMER_LEFT_7=718,20
PRIMER_RIGHT_7=1561,20
PRIMER_LEFT_7_TM=57.571
PRIMER_RIGHT_7_TM=60.762
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=11.1000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=844
PRIMER_PAIR_8_PENALTY=3.231053
PRIMER_LEFT_8_PENALTY=2.429424
PRIMER_RIGHT_8_PENALTY=0.801629
PRIMER_LEFT_8_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_8_SEQUENCE=GTTGCCGTCTCCTTTATCGT
PRIMER_LEFT_8=719,20
PRIMER_RIGHT_8=1549,20
PRIMER_LEFT_8_TM=57.571
PRIMER_RIGHT_8_TM=59.198
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.0000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=831
PRIMER_PAIR_9_PENALTY=3.231053
PRIMER_LEFT_9_PENALTY=2.429424
PRIMER_RIGHT_9_PENALTY=0.801629
PRIMER_LEFT_9_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_9_SEQUENCE=GTTGCCGTCTCCTTTATCGT
PRIMER_LEFT_9=718,20
PRIMER_RIGHT_9=1549,20
PRIMER_LEFT_9_TM=57.571
PRIMER_RIGHT_9_TM=59.198
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=8.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=832
PRIMER_PAIR_10_PENALTY=3.356155
PRIMER_LEFT_10_PENALTY=2.429424
PRIMER_RIGHT_10_PENALTY=0.926731
PRIMER_LEFT_10_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_10_SEQUENCE=AAAACAAAGTGGGGTCGAAG
PRIMER_LEFT_10=719,20
PRIMER_RIGHT_10=1639,20
PRIMER_LEFT_10_TM=57.571
PRIMER_RIGHT_10_TM=59.073
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=921
PRIMER_PAIR_11_PENALTY=3.356155
PRIMER_LEFT_11_PENALTY=2.429424
PRIMER_RIGHT_11_PENALTY=0.926731
PRIMER_LEFT_11_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_11_SEQUENCE=AAAACAAAGTGGGGTCGAAG
PRIMER_LEFT_11=718,20
PRIMER_RIGHT_11=1639,20
PRIMER_LEFT_11_TM=57.571
PRIMER_RIGHT_11_TM=59.073
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=922
PRIMER_PAIR_12_PENALTY=3.401739
PRIMER_LEFT_12_PENALTY=2.429424
PRIMER_RIGHT_12_PENALTY=0.972315
PRIMER_LEFT_12_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_12_SEQUENCE=TGGGGTCGAAGTAGGAAAAC
PRIMER_LEFT_12=719,20
PRIMER_RIGHT_12=1630,20
PRIMER_LEFT_12_TM=57.571
PRIMER_RIGHT_12_TM=59.028
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=912
PRIMER_PAIR_13_PENALTY=3.401739
PRIMER_LEFT_13_PENALTY=2.429424
PRIMER_RIGHT_13_PENALTY=0.972315
PRIMER_LEFT_13_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_13_SEQUENCE=GTGGGGTCGAAGTAGGAAAA
PRIMER_LEFT_13=719,20
PRIMER_RIGHT_13=1631,20
PRIMER_LEFT_13_TM=57.571
PRIMER_RIGHT_13_TM=59.028
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=913
PRIMER_PAIR_14_PENALTY=3.401739
PRIMER_LEFT_14_PENALTY=2.429424
PRIMER_RIGHT_14_PENALTY=0.972315
PRIMER_LEFT_14_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_14_SEQUENCE=TGGGGTCGAAGTAGGAAAAC
PRIMER_LEFT_14=718,20
PRIMER_RIGHT_14=1630,20
PRIMER_LEFT_14_TM=57.571
PRIMER_RIGHT_14_TM=59.028
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=913
PRIMER_PAIR_15_PENALTY=3.401739
PRIMER_LEFT_15_PENALTY=2.429424
PRIMER_RIGHT_15_PENALTY=0.972315
PRIMER_LEFT_15_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_15_SEQUENCE=GTGGGGTCGAAGTAGGAAAA
PRIMER_LEFT_15=718,20
PRIMER_RIGHT_15=1631,20
PRIMER_LEFT_15_TM=57.571
PRIMER_RIGHT_15_TM=59.028
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.3000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=914
PRIMER_PAIR_16_PENALTY=3.415598
PRIMER_LEFT_16_PENALTY=2.429424
PRIMER_RIGHT_16_PENALTY=0.986173
PRIMER_LEFT_16_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_16_SEQUENCE=GATGGTACTTGTGTCGTGCC
PRIMER_LEFT_16=718,20
PRIMER_RIGHT_16=1576,20
PRIMER_LEFT_16_TM=57.571
PRIMER_RIGHT_16_TM=59.014
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=859
PRIMER_PAIR_17_PENALTY=3.445645
PRIMER_LEFT_17_PENALTY=2.429424
PRIMER_RIGHT_17_PENALTY=1.016221
PRIMER_LEFT_17_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_17_SEQUENCE=TGGGGTCGAAGTAGGAAAACT
PRIMER_LEFT_17=719,20
PRIMER_RIGHT_17=1630,21
PRIMER_LEFT_17_TM=57.571
PRIMER_RIGHT_17_TM=59.984
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=912
PRIMER_PAIR_18_PENALTY=3.445645
PRIMER_LEFT_18_PENALTY=2.429424
PRIMER_RIGHT_18_PENALTY=1.016221
PRIMER_LEFT_18_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_18_SEQUENCE=AGTGGGGTCGAAGTAGGAAAA
PRIMER_LEFT_18=719,20
PRIMER_RIGHT_18=1632,21
PRIMER_LEFT_18_TM=57.571
PRIMER_RIGHT_18_TM=59.984
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=914
PRIMER_PAIR_19_PENALTY=3.445645
PRIMER_LEFT_19_PENALTY=2.429424
PRIMER_RIGHT_19_PENALTY=1.016221
PRIMER_LEFT_19_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_19_SEQUENCE=AAGTGGGGTCGAAGTAGGAAA
PRIMER_LEFT_19=719,20
PRIMER_RIGHT_19=1633,21
PRIMER_LEFT_19_TM=57.571
PRIMER_RIGHT_19_TM=59.984
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=915
PRIMER_PAIR_20_PENALTY=3.445645
PRIMER_LEFT_20_PENALTY=2.429424
PRIMER_RIGHT_20_PENALTY=1.016221
PRIMER_LEFT_20_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_20_SEQUENCE=AAAGTGGGGTCGAAGTAGGAA
PRIMER_LEFT_20=719,20
PRIMER_RIGHT_20=1634,21
PRIMER_LEFT_20_TM=57.571
PRIMER_RIGHT_20_TM=59.984
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=916
PRIMER_PAIR_21_PENALTY=3.445645
PRIMER_LEFT_21_PENALTY=2.429424
PRIMER_RIGHT_21_PENALTY=1.016221
PRIMER_LEFT_21_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_21_SEQUENCE=TGGGGTCGAAGTAGGAAAACT
PRIMER_LEFT_21=718,20
PRIMER_RIGHT_21=1630,21
PRIMER_LEFT_21_TM=57.571
PRIMER_RIGHT_21_TM=59.984
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=913
PRIMER_PAIR_22_PENALTY=3.445645
PRIMER_LEFT_22_PENALTY=2.429424
PRIMER_RIGHT_22_PENALTY=1.016221
PRIMER_LEFT_22_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_22_SEQUENCE=AGTGGGGTCGAAGTAGGAAAA
PRIMER_LEFT_22=718,20
PRIMER_RIGHT_22=1632,21
PRIMER_LEFT_22_TM=57.571
PRIMER_RIGHT_22_TM=59.984
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.3000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=915
PRIMER_PAIR_23_PENALTY=3.445645
PRIMER_LEFT_23_PENALTY=2.429424
PRIMER_RIGHT_23_PENALTY=1.016221
PRIMER_LEFT_23_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_23_SEQUENCE=AAGTGGGGTCGAAGTAGGAAA
PRIMER_LEFT_23=718,20
PRIMER_RIGHT_23=1633,21
PRIMER_LEFT_23_TM=57.571
PRIMER_RIGHT_23_TM=59.984
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=916
PRIMER_PAIR_24_PENALTY=3.445645
PRIMER_LEFT_24_PENALTY=2.429424
PRIMER_RIGHT_24_PENALTY=1.016221
PRIMER_LEFT_24_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_24_SEQUENCE=AAAGTGGGGTCGAAGTAGGAA
PRIMER_LEFT_24=718,20
PRIMER_RIGHT_24=1634,21
PRIMER_LEFT_24_TM=57.571
PRIMER_RIGHT_24_TM=59.984
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=917
PRIMER_PAIR_25_PENALTY=3.459390
PRIMER_LEFT_25_PENALTY=2.429424
PRIMER_RIGHT_25_PENALTY=1.029965
PRIMER_LEFT_25_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_25_SEQUENCE=AAACGAAAAACAAAGTGGGG
PRIMER_LEFT_25=719,20
PRIMER_RIGHT_25=1645,20
PRIMER_LEFT_25_TM=57.571
PRIMER_RIGHT_25_TM=58.970
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=11.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=927
PRIMER_PAIR_26_PENALTY=3.459390
PRIMER_LEFT_26_PENALTY=2.429424
PRIMER_RIGHT_26_PENALTY=1.029965
PRIMER_LEFT_26_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_26_SEQUENCE=AAACGAAAAACAAAGTGGGG
PRIMER_LEFT_26=718,20
PRIMER_RIGHT_26=1645,20
PRIMER_LEFT_26_TM=57.571
PRIMER_RIGHT_26_TM=58.970
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=11.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=928
PRIMER_PAIR_27_PENALTY=3.495789
PRIMER_LEFT_27_PENALTY=2.429424
PRIMER_RIGHT_27_PENALTY=1.066365
PRIMER_LEFT_27_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_27_SEQUENCE=ACGAAAAACAAAGTGGGGTC
PRIMER_LEFT_27=719,20
PRIMER_RIGHT_27=1643,20
PRIMER_LEFT_27_TM=57.571
PRIMER_RIGHT_27_TM=58.934
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=9.1000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=925
PRIMER_PAIR_28_PENALTY=3.495789
PRIMER_LEFT_28_PENALTY=2.429424
PRIMER_RIGHT_28_PENALTY=1.066365
PRIMER_LEFT_28_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_28_SEQUENCE=ACGAAAAACAAAGTGGGGTC
PRIMER_LEFT_28=718,20
PRIMER_RIGHT_28=1643,20
PRIMER_LEFT_28_TM=57.571
PRIMER_RIGHT_28_TM=58.934
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=9.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=926
PRIMER_PAIR_29_PENALTY=3.536290
PRIMER_LEFT_29_PENALTY=2.429424
PRIMER_RIGHT_29_PENALTY=1.106866
PRIMER_LEFT_29_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_29_SEQUENCE=AAAACAAAGTGGGGTCGAAGT
PRIMER_LEFT_29=719,20
PRIMER_RIGHT_29=1639,21
PRIMER_LEFT_29_TM=57.571
PRIMER_RIGHT_29_TM=59.893
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=42.857
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=6.4000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=921
PRIMER_PAIR_30_PENALTY=3.536290
PRIMER_LEFT_30_PENALTY=2.429424
PRIMER_RIGHT_30_PENALTY=1.106866
PRIMER_LEFT_30_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_30_SEQUENCE=AAAACAAAGTGGGGTCGAAGT
PRIMER_LEFT_30=718,20
PRIMER_RIGHT_30=1639,21
PRIMER_LEFT_30_TM=57.571
PRIMER_RIGHT_30_TM=59.893
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=922
PRIMER_PAIR_31_PENALTY=3.573175
PRIMER_LEFT_31_PENALTY=2.429424
PRIMER_RIGHT_31_PENALTY=1.143751
PRIMER_LEFT_31_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_31_SEQUENCE=GTGGGGTCGAAGTAGGAAAAC
PRIMER_LEFT_31=719,20
PRIMER_RIGHT_31=1631,21
PRIMER_LEFT_31_TM=57.571
PRIMER_RIGHT_31_TM=59.856
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=52.381
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=913
PRIMER_PAIR_32_PENALTY=3.573175
PRIMER_LEFT_32_PENALTY=2.429424
PRIMER_RIGHT_32_PENALTY=1.143751
PRIMER_LEFT_32_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_32_SEQUENCE=GTGGGGTCGAAGTAGGAAAAC
PRIMER_LEFT_32=718,20
PRIMER_RIGHT_32=1631,21
PRIMER_LEFT_32_TM=57.571
PRIMER_RIGHT_32_TM=59.856
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=914
PRIMER_PAIR_33_PENALTY=3.677449
PRIMER_LEFT_33_PENALTY=2.429424
PRIMER_RIGHT_33_PENALTY=1.248025
PRIMER_LEFT_33_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_33_SEQUENCE=AACGAAAAACAAAGTGGGGTC
PRIMER_LEFT_33=719,20
PRIMER_RIGHT_33=1644,21
PRIMER_LEFT_33_TM=57.571
PRIMER_RIGHT_33_TM=60.248
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=9.1000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=926
PRIMER_PAIR_34_PENALTY=3.677449
PRIMER_LEFT_34_PENALTY=2.429424
PRIMER_RIGHT_34_PENALTY=1.248025
PRIMER_LEFT_34_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_34_SEQUENCE=AACGAAAAACAAAGTGGGGTC
PRIMER_LEFT_34=718,20
PRIMER_RIGHT_34=1644,21
PRIMER_LEFT_34_TM=57.571
PRIMER_RIGHT_34_TM=60.248
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=9.1000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=927
PRIMER_PAIR_35_PENALTY=3.785331
PRIMER_LEFT_35_PENALTY=2.429424
PRIMER_RIGHT_35_PENALTY=1.355907
PRIMER_LEFT_35_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_35_SEQUENCE=AGTGGGGTCGAAGTAGGAAA
PRIMER_LEFT_35=719,20
PRIMER_RIGHT_35=1632,20
PRIMER_LEFT_35_TM=57.571
PRIMER_RIGHT_35_TM=58.644
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=8.5000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=914
PRIMER_PAIR_36_PENALTY=3.785331
PRIMER_LEFT_36_PENALTY=2.429424
PRIMER_RIGHT_36_PENALTY=1.355907
PRIMER_LEFT_36_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_36_SEQUENCE=AAGTGGGGTCGAAGTAGGAA
PRIMER_LEFT_36=719,20
PRIMER_RIGHT_36=1633,20
PRIMER_LEFT_36_TM=57.571
PRIMER_RIGHT_36_TM=58.644
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=915
PRIMER_PAIR_37_PENALTY=3.785331
PRIMER_LEFT_37_PENALTY=2.429424
PRIMER_RIGHT_37_PENALTY=1.355907
PRIMER_LEFT_37_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_37_SEQUENCE=AAAGTGGGGTCGAAGTAGGA
PRIMER_LEFT_37=719,20
PRIMER_RIGHT_37=1634,20
PRIMER_LEFT_37_TM=57.571
PRIMER_RIGHT_37_TM=58.644
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=916
PRIMER_PAIR_38_PENALTY=3.785331
PRIMER_LEFT_38_PENALTY=2.429424
PRIMER_RIGHT_38_PENALTY=1.355907
PRIMER_LEFT_38_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_38_SEQUENCE=AGTGGGGTCGAAGTAGGAAA
PRIMER_LEFT_38=718,20
PRIMER_RIGHT_38=1632,20
PRIMER_LEFT_38_TM=57.571
PRIMER_RIGHT_38_TM=58.644
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.5000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=915
PRIMER_PAIR_39_PENALTY=3.785331
PRIMER_LEFT_39_PENALTY=2.429424
PRIMER_RIGHT_39_PENALTY=1.355907
PRIMER_LEFT_39_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_39_SEQUENCE=AAGTGGGGTCGAAGTAGGAA
PRIMER_LEFT_39=718,20
PRIMER_RIGHT_39=1633,20
PRIMER_LEFT_39_TM=57.571
PRIMER_RIGHT_39_TM=58.644
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=916
PRIMER_PAIR_40_PENALTY=3.785331
PRIMER_LEFT_40_PENALTY=2.429424
PRIMER_RIGHT_40_PENALTY=1.355907
PRIMER_LEFT_40_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_40_SEQUENCE=AAAGTGGGGTCGAAGTAGGA
PRIMER_LEFT_40=718,20
PRIMER_RIGHT_40=1634,20
PRIMER_LEFT_40_TM=57.571
PRIMER_RIGHT_40_TM=58.644
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=917
PRIMER_PAIR_41_PENALTY=3.800124
PRIMER_LEFT_41_PENALTY=2.429424
PRIMER_RIGHT_41_PENALTY=1.370699
PRIMER_LEFT_41_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_41_SEQUENCE=AAAAACAAAGTGGGGTCGAAG
PRIMER_LEFT_41=719,20
PRIMER_RIGHT_41=1640,21
PRIMER_LEFT_41_TM=57.571
PRIMER_RIGHT_41_TM=60.371
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=8.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=922
PRIMER_PAIR_42_PENALTY=3.800124
PRIMER_LEFT_42_PENALTY=2.429424
PRIMER_RIGHT_42_PENALTY=1.370699
PRIMER_LEFT_42_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_42_SEQUENCE=AAAAACAAAGTGGGGTCGAAG
PRIMER_LEFT_42=718,20
PRIMER_RIGHT_42=1640,21
PRIMER_LEFT_42_TM=57.571
PRIMER_RIGHT_42_TM=60.371
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=42.857
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=923
PRIMER_PAIR_43_PENALTY=3.830261
PRIMER_LEFT_43_PENALTY=2.429424
PRIMER_RIGHT_43_PENALTY=1.400837
PRIMER_LEFT_43_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_43_SEQUENCE=ACAAAGTGGGGTCGAAGTAGG
PRIMER_LEFT_43=719,20
PRIMER_RIGHT_43=1636,21
PRIMER_LEFT_43_TM=57.571
PRIMER_RIGHT_43_TM=60.401
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=918
PRIMER_PAIR_44_PENALTY=3.830261
PRIMER_LEFT_44_PENALTY=2.429424
PRIMER_RIGHT_44_PENALTY=1.400837
PRIMER_LEFT_44_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_44_SEQUENCE=ACAAAGTGGGGTCGAAGTAGG
PRIMER_LEFT_44=718,20
PRIMER_RIGHT_44=1636,21
PRIMER_LEFT_44_TM=57.571
PRIMER_RIGHT_44_TM=60.401
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.0000
PRIMER_RIGHT_44_END_STABILITY=6.9000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=919
PRIMER_PAIR_45_PENALTY=3.879718
PRIMER_LEFT_45_PENALTY=2.429424
PRIMER_RIGHT_45_PENALTY=1.450294
PRIMER_LEFT_45_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_45_SEQUENCE=AAACAAAGTGGGGTCGAAGT
PRIMER_LEFT_45=719,20
PRIMER_RIGHT_45=1638,20
PRIMER_LEFT_45_TM=57.571
PRIMER_RIGHT_45_TM=58.550
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=920
PRIMER_PAIR_46_PENALTY=3.879718
PRIMER_LEFT_46_PENALTY=2.429424
PRIMER_RIGHT_46_PENALTY=1.450294
PRIMER_LEFT_46_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_46_SEQUENCE=AAACAAAGTGGGGTCGAAGT
PRIMER_LEFT_46=718,20
PRIMER_RIGHT_46=1638,20
PRIMER_LEFT_46_TM=57.571
PRIMER_RIGHT_46_TM=58.550
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=921
PRIMER_PAIR_47_PENALTY=3.894827
PRIMER_LEFT_47_PENALTY=2.429424
PRIMER_RIGHT_47_PENALTY=1.465403
PRIMER_LEFT_47_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_47_SEQUENCE=GCCGTCTCCTTTATCGTCATT
PRIMER_LEFT_47=719,20
PRIMER_RIGHT_47=1546,21
PRIMER_LEFT_47_TM=57.571
PRIMER_RIGHT_47_TM=60.465
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=828
PRIMER_PAIR_48_PENALTY=3.894827
PRIMER_LEFT_48_PENALTY=2.429424
PRIMER_RIGHT_48_PENALTY=1.465403
PRIMER_LEFT_48_SEQUENCE=AACAACCAGCACAATCAACA
PRIMER_RIGHT_48_SEQUENCE=GCCGTCTCCTTTATCGTCATT
PRIMER_LEFT_48=718,20
PRIMER_RIGHT_48=1546,21
PRIMER_LEFT_48_TM=57.571
PRIMER_RIGHT_48_TM=60.465
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=829
PRIMER_PAIR_49_PENALTY=3.968609
PRIMER_LEFT_49_PENALTY=2.429424
PRIMER_RIGHT_49_PENALTY=1.539185
PRIMER_LEFT_49_SEQUENCE=ACAACCAGCACAATCAACAA
PRIMER_RIGHT_49_SEQUENCE=AACGAAAAACAAAGTGGGGT
PRIMER_LEFT_49=719,20
PRIMER_RIGHT_49=1644,20
PRIMER_LEFT_49_TM=57.571
PRIMER_RIGHT_49_TM=58.461
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=10.6000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=926
=
SEQUENCE_ID=MAGIC.426_3_17535083_17535233_zu-0_wil-2_65598_225156_TRUE
SEQUENCE_TARGET=950,250
SEQUENCE_TEMPLATE=GGTATAANAAGTTGAATGGGACTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNCGGCTCTCTACCANNNGATATTGGNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNATCTCCCACAAACNTTGGGAAANTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNCCANATNTAAGAGGGTTGNTGGGTNTNAGAAGNGTTGATTNNNNNNNNNNNNNNNNNTCNGGAAGTATNCCTGNATATTTTGCAAACTTNTCCAANTTGGANTATCTCAATCTATCNATTAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACNCTACAATAGTTTNNNNNNNNNNNNNNNNNNNNNTATGTGGAGGCANCAAGNATCTGNNNNNNAAGCCATNCNTNGCCCAAGAACCACCAGTGGAGACNAAGCATTCCTCTCATTTAAAGAAAGTTGCAATTNTAGTAAGCATAGGCATAGCTTTGCTATTGCTGTTGGTCATANNNNNNNNGNTNNNGNGTTGGTTCAGAAAAAGAANGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNTTTTCCATGAGAAGATAAGNTATGGAGATCTNCNAAATGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAGGNAGTTTTGGTACTGTGTTNAAGGCATTGCTNCCGACGGAGANCAAGATTGTTGCAGTGAAAGTTNTAAACATGCAGAGACGTGGAGCTATGAAGAGCTTTATGGCAGAATGTGAATCCTTGAAAGACACAAGGCATCGTAATCTTGTGAANTTNNTGACAGCTTGCGCNAGTANTGATTTCCANGGNAACGAATTCAGAGCNCTNATCTATGAGTNCNTGCCGAATGGAAGCNTGGATATGTGGCTNCACCCAGAGGAAGTNGAAGAGATACNTAGGCCCNCANGANCCTTGACACTTNTTGAAAGGCTNAATATTGTCATAGANGTGGCTTCTGTTTTGGACTATCTTCATGTTCANTGTCATGAGCCTATAGCNCATTGCGATCTNAAGCCAAGCAACGTCCTTCTAGANGATGATTTNACNGCCCATGTNAGTGATTTTGGTCTGGCTCGGCTTCTNCTCAAATTCGACAAGGAGTCATTCCTTAACCAACTAAGCTCAGCCGGAGTTAGAGGNACCATCGGCTATGCCGCACCAGGNAAAAATATCACACATCTGTAATGTTCTATGAAGAAACTTGGTATATNTTTTNTCGTTACAATCTTGTTCTGTGAATTATGGTGCAGAATATGGAATGGGAGGACAACCATCAATACATGGTGATGTGTATAGCTTTGGGGTTCTCCTTTTAGAAATGTTCACCGGGAAACGACCAACTGATGAGTTATTTGGGGGAAATTTAACACTACATAGCTACACCAAGTTAGCGTTGCCAGAAAAAGTATTCGAAATTGCAGANAAAGCGATTCTTCACATTGGTCTTAGAGTTGGTTTCCGTACTGCTGAGTGCTTGACACTGGTTTTGGAGGTGGGACTTAGGTGCTGTGAAGAATATCCTACGAACCGGTTGGCAACGAGTGAAGTCGCAAAAGAGTTGATCTCAATCAGAGAAAGGTTCTTTAAAACCAGAAGAACACCTAGACGTTGAAGTTTTTATGGTTTTGCCTACAAANTTTGCAGTCCACTCAAAANGCCTATATAACATTGTCTTTTACAATTTGTGTTTTCTTCTTCTCTATTAAACATACTTATGCTNTACGTGTTTNTAAGCNCTATTAAAACATANTAAGATGGCTTGCAGAATATTGTTAATGAATGCTTGTGTTTTTTGCAGACAATATATGTATGTTGTGTANCCAGTTTTATCATATATATCANTGTTCCCAACTTTATTTGGATTTTCTCAGTTTTTCGTAGCTTAAAAATGAATTACAAACTTTCATGATTTAAAAGGCAAACGTAACTCAATTCTAGTTGTNCNTCACAATGACTGTCTTTATGAACTCCATAACTGTTGAAACTAATTGACTTTGGTNCTCAGTATAACCATGATTAGCTNCTTCCACAATC
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.186871
PRIMER_LEFT_0_PENALTY=0.159749
PRIMER_RIGHT_0_PENALTY=0.027122
PRIMER_LEFT_0_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_0_SEQUENCE=TTTTGCGACTTCACTCGTTG
PRIMER_LEFT_0=810,20
PRIMER_RIGHT_0=1670,20
PRIMER_LEFT_0_TM=60.160
PRIMER_RIGHT_0_TM=60.027
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.9000
PRIMER_RIGHT_0_END_STABILITY=8.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=861
PRIMER_PAIR_1_PENALTY=0.200520
PRIMER_LEFT_1_PENALTY=0.159749
PRIMER_RIGHT_1_PENALTY=0.040772
PRIMER_LEFT_1_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_1_SEQUENCE=CACCTAAGTCCCACCTCCAA
PRIMER_LEFT_1=810,20
PRIMER_RIGHT_1=1621,20
PRIMER_LEFT_1_TM=60.160
PRIMER_RIGHT_1_TM=59.959
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PAIR_1_PRODUCT_SIZE=812
PRIMER_PAIR_2_PENALTY=0.378932
PRIMER_LEFT_2_PENALTY=0.351810
PRIMER_RIGHT_2_PENALTY=0.027122
PRIMER_LEFT_2_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_2_SEQUENCE=TTTTGCGACTTCACTCGTTG
PRIMER_LEFT_2=840,20
PRIMER_RIGHT_2=1670,20
PRIMER_LEFT_2_TM=59.648
PRIMER_RIGHT_2_TM=60.027
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=831
PRIMER_PAIR_3_PENALTY=0.392582
PRIMER_LEFT_3_PENALTY=0.351810
PRIMER_RIGHT_3_PENALTY=0.040772
PRIMER_LEFT_3_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_3_SEQUENCE=CACCTAAGTCCCACCTCCAA
PRIMER_LEFT_3=840,20
PRIMER_RIGHT_3=1621,20
PRIMER_LEFT_3_TM=59.648
PRIMER_RIGHT_3_TM=59.959
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=782
PRIMER_PAIR_4_PENALTY=0.437734
PRIMER_LEFT_4_PENALTY=0.159749
PRIMER_RIGHT_4_PENALTY=0.277985
PRIMER_LEFT_4_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_4_SEQUENCE=TCCAAAACCAGTGTCAAGCA
PRIMER_LEFT_4=810,20
PRIMER_RIGHT_4=1606,20
PRIMER_LEFT_4_TM=60.160
PRIMER_RIGHT_4_TM=60.278
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=797
PRIMER_PAIR_5_PENALTY=0.559074
PRIMER_LEFT_5_PENALTY=0.159749
PRIMER_RIGHT_5_PENALTY=0.399325
PRIMER_LEFT_5_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_5_SEQUENCE=CCACCTCCAAAACCAGTGTC
PRIMER_LEFT_5=810,20
PRIMER_RIGHT_5=1611,20
PRIMER_LEFT_5_TM=60.160
PRIMER_RIGHT_5_TM=60.399
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=5.9000
PRIMER_RIGHT_5_END_STABILITY=6.1000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=802
PRIMER_PAIR_6_PENALTY=0.612338
PRIMER_LEFT_6_PENALTY=0.585216
PRIMER_RIGHT_6_PENALTY=0.027122
PRIMER_LEFT_6_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_6_SEQUENCE=TTTTGCGACTTCACTCGTTG
PRIMER_LEFT_6=854,20
PRIMER_RIGHT_6=1670,20
PRIMER_LEFT_6_TM=59.415
PRIMER_RIGHT_6_TM=60.027
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=8.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=817
PRIMER_PAIR_7_PENALTY=0.625987
PRIMER_LEFT_7_PENALTY=0.585216
PRIMER_RIGHT_7_PENALTY=0.040772
PRIMER_LEFT_7_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_7_SEQUENCE=CACCTAAGTCCCACCTCCAA
PRIMER_LEFT_7=854,20
PRIMER_RIGHT_7=1621,20
PRIMER_LEFT_7_TM=59.415
PRIMER_RIGHT_7_TM=59.959
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=768
PRIMER_PAIR_8_PENALTY=0.630657
PRIMER_LEFT_8_PENALTY=0.351810
PRIMER_RIGHT_8_PENALTY=0.278847
PRIMER_LEFT_8_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_8_SEQUENCE=CAGCACCTAAGTCCCACCTC
PRIMER_LEFT_8=840,20
PRIMER_RIGHT_8=1624,20
PRIMER_LEFT_8_TM=59.648
PRIMER_RIGHT_8_TM=59.721
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=60.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=785
PRIMER_PAIR_9_PENALTY=0.670380
PRIMER_LEFT_9_PENALTY=0.159749
PRIMER_RIGHT_9_PENALTY=0.510631
PRIMER_LEFT_9_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_9_SEQUENCE=AGCACCTAAGTCCCACCTCC
PRIMER_LEFT_9=810,20
PRIMER_RIGHT_9=1623,20
PRIMER_LEFT_9_TM=60.160
PRIMER_RIGHT_9_TM=60.511
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=60.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=5.9000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PAIR_9_PRODUCT_SIZE=814
PRIMER_PAIR_10_PENALTY=0.740578
PRIMER_LEFT_10_PENALTY=0.585216
PRIMER_RIGHT_10_PENALTY=0.155362
PRIMER_LEFT_10_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_10_SEQUENCE=CCCATTCCATATTCTGCACC
PRIMER_LEFT_10=854,20
PRIMER_RIGHT_10=1378,20
PRIMER_LEFT_10_TM=59.415
PRIMER_RIGHT_10_TM=60.155
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=525
PRIMER_PAIR_11_PENALTY=0.751135
PRIMER_LEFT_11_PENALTY=0.351810
PRIMER_RIGHT_11_PENALTY=0.399325
PRIMER_LEFT_11_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_11_SEQUENCE=CCACCTCCAAAACCAGTGTC
PRIMER_LEFT_11=840,20
PRIMER_RIGHT_11=1611,20
PRIMER_LEFT_11_TM=59.648
PRIMER_RIGHT_11_TM=60.399
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=6.1000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=772
PRIMER_PAIR_12_PENALTY=0.862441
PRIMER_LEFT_12_PENALTY=0.351810
PRIMER_RIGHT_12_PENALTY=0.510631
PRIMER_LEFT_12_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_12_SEQUENCE=AGCACCTAAGTCCCACCTCC
PRIMER_LEFT_12=840,20
PRIMER_RIGHT_12=1623,20
PRIMER_LEFT_12_TM=59.648
PRIMER_RIGHT_12_TM=60.511
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=60.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=784
PRIMER_PAIR_13_PENALTY=0.863201
PRIMER_LEFT_13_PENALTY=0.585216
PRIMER_RIGHT_13_PENALTY=0.277985
PRIMER_LEFT_13_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_13_SEQUENCE=TCCAAAACCAGTGTCAAGCA
PRIMER_LEFT_13=854,20
PRIMER_RIGHT_13=1606,20
PRIMER_LEFT_13_TM=59.415
PRIMER_RIGHT_13_TM=60.278
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=753
PRIMER_PAIR_14_PENALTY=0.864063
PRIMER_LEFT_14_PENALTY=0.585216
PRIMER_RIGHT_14_PENALTY=0.278847
PRIMER_LEFT_14_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_14_SEQUENCE=CAGCACCTAAGTCCCACCTC
PRIMER_LEFT_14=854,20
PRIMER_RIGHT_14=1624,20
PRIMER_LEFT_14_TM=59.415
PRIMER_RIGHT_14_TM=59.721
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=60.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=771
PRIMER_PAIR_15_PENALTY=0.884330
PRIMER_LEFT_15_PENALTY=0.857208
PRIMER_RIGHT_15_PENALTY=0.027122
PRIMER_LEFT_15_SEQUENCE=GCAGAGACGTGGAGCTATGA
PRIMER_RIGHT_15_SEQUENCE=TTTTGCGACTTCACTCGTTG
PRIMER_LEFT_15=811,20
PRIMER_RIGHT_15=1670,20
PRIMER_LEFT_15_TM=59.143
PRIMER_RIGHT_15_TM=60.027
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=5.9000
PRIMER_RIGHT_15_END_STABILITY=8.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=860
PRIMER_PAIR_16_PENALTY=0.918917
PRIMER_LEFT_16_PENALTY=0.351810
PRIMER_RIGHT_16_PENALTY=0.567107
PRIMER_LEFT_16_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_16_SEQUENCE=CACAGCACCTAAGTCCCACC
PRIMER_LEFT_16=840,20
PRIMER_RIGHT_16=1626,20
PRIMER_LEFT_16_TM=59.648
PRIMER_RIGHT_16_TM=60.567
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=60.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=787
PRIMER_PAIR_17_PENALTY=0.984541
PRIMER_LEFT_17_PENALTY=0.585216
PRIMER_RIGHT_17_PENALTY=0.399325
PRIMER_LEFT_17_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_17_SEQUENCE=CCACCTCCAAAACCAGTGTC
PRIMER_LEFT_17=854,20
PRIMER_RIGHT_17=1611,20
PRIMER_LEFT_17_TM=59.415
PRIMER_RIGHT_17_TM=60.399
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=6.1000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=758
PRIMER_PAIR_18_PENALTY=0.986722
PRIMER_LEFT_18_PENALTY=0.159749
PRIMER_RIGHT_18_PENALTY=0.826973
PRIMER_LEFT_18_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_18_SEQUENCE=TCTGGCAACGCTAACTTGGT
PRIMER_LEFT_18=810,20
PRIMER_RIGHT_18=1516,20
PRIMER_LEFT_18_TM=60.160
PRIMER_RIGHT_18_TM=60.827
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=5.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=707
PRIMER_PAIR_19_PENALTY=1.012570
PRIMER_LEFT_19_PENALTY=0.857208
PRIMER_RIGHT_19_PENALTY=0.155362
PRIMER_LEFT_19_SEQUENCE=GCAGAGACGTGGAGCTATGA
PRIMER_RIGHT_19_SEQUENCE=CCCATTCCATATTCTGCACC
PRIMER_LEFT_19=811,20
PRIMER_RIGHT_19=1378,20
PRIMER_LEFT_19_TM=59.143
PRIMER_RIGHT_19_TM=60.155
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=568
PRIMER_PAIR_20_PENALTY=1.062464
PRIMER_LEFT_20_PENALTY=0.159749
PRIMER_RIGHT_20_PENALTY=0.902715
PRIMER_LEFT_20_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_20_SEQUENCE=TCATCAGTTGGTCGTTTCCC
PRIMER_LEFT_20=810,20
PRIMER_RIGHT_20=1462,20
PRIMER_LEFT_20_TM=60.160
PRIMER_RIGHT_20_TM=60.903
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=5.9000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=653
PRIMER_PAIR_21_PENALTY=1.063678
PRIMER_LEFT_21_PENALTY=0.351810
PRIMER_RIGHT_21_PENALTY=0.711868
PRIMER_LEFT_21_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_21_SEQUENCE=TTCACAGCACCTAAGTCCCA
PRIMER_LEFT_21=840,20
PRIMER_RIGHT_21=1628,20
PRIMER_LEFT_21_TM=59.648
PRIMER_RIGHT_21_TM=59.288
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=6.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=789
PRIMER_PAIR_22_PENALTY=1.079818
PRIMER_LEFT_22_PENALTY=1.052696
PRIMER_RIGHT_22_PENALTY=0.027122
PRIMER_LEFT_22_SEQUENCE=ATGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_22_SEQUENCE=TTTTGCGACTTCACTCGTTG
PRIMER_LEFT_22=839,21
PRIMER_RIGHT_22=1670,20
PRIMER_LEFT_22_TM=59.947
PRIMER_RIGHT_22_TM=60.027
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=8.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=832
PRIMER_PAIR_23_PENALTY=1.093468
PRIMER_LEFT_23_PENALTY=1.052696
PRIMER_RIGHT_23_PENALTY=0.040772
PRIMER_LEFT_23_SEQUENCE=ATGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_23_SEQUENCE=CACCTAAGTCCCACCTCCAA
PRIMER_LEFT_23=839,21
PRIMER_RIGHT_23=1621,20
PRIMER_LEFT_23_TM=59.947
PRIMER_RIGHT_23_TM=59.959
PRIMER_LEFT_23_GC_PERCENT=42.857
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=8.5000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=783
PRIMER_PAIR_24_PENALTY=1.095847
PRIMER_LEFT_24_PENALTY=0.585216
PRIMER_RIGHT_24_PENALTY=0.510631
PRIMER_LEFT_24_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_24_SEQUENCE=AGCACCTAAGTCCCACCTCC
PRIMER_LEFT_24=854,20
PRIMER_RIGHT_24=1623,20
PRIMER_LEFT_24_TM=59.415
PRIMER_RIGHT_24_TM=60.511
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=60.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=770
PRIMER_PAIR_25_PENALTY=1.135193
PRIMER_LEFT_25_PENALTY=0.857208
PRIMER_RIGHT_25_PENALTY=0.277985
PRIMER_LEFT_25_SEQUENCE=GCAGAGACGTGGAGCTATGA
PRIMER_RIGHT_25_SEQUENCE=TCCAAAACCAGTGTCAAGCA
PRIMER_LEFT_25=811,20
PRIMER_RIGHT_25=1606,20
PRIMER_LEFT_25_TM=59.143
PRIMER_RIGHT_25_TM=60.278
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=5.9000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=796
PRIMER_PAIR_26_PENALTY=1.135265
PRIMER_LEFT_26_PENALTY=0.159749
PRIMER_RIGHT_26_PENALTY=0.975516
PRIMER_LEFT_26_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_26_SEQUENCE=CCTAAGTCCCACCTCCAAAA
PRIMER_LEFT_26=810,20
PRIMER_RIGHT_26=1619,20
PRIMER_LEFT_26_TM=60.160
PRIMER_RIGHT_26_TM=59.024
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=5.9000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=810
PRIMER_PAIR_27_PENALTY=1.136056
PRIMER_LEFT_27_PENALTY=0.857208
PRIMER_RIGHT_27_PENALTY=0.278847
PRIMER_LEFT_27_SEQUENCE=GCAGAGACGTGGAGCTATGA
PRIMER_RIGHT_27_SEQUENCE=CAGCACCTAAGTCCCACCTC
PRIMER_LEFT_27=811,20
PRIMER_RIGHT_27=1624,20
PRIMER_LEFT_27_TM=59.143
PRIMER_RIGHT_27_TM=59.721
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=60.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=5.9000
PRIMER_RIGHT_27_END_STABILITY=7.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=814
PRIMER_PAIR_28_PENALTY=1.152322
PRIMER_LEFT_28_PENALTY=0.585216
PRIMER_RIGHT_28_PENALTY=0.567107
PRIMER_LEFT_28_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_28_SEQUENCE=CACAGCACCTAAGTCCCACC
PRIMER_LEFT_28=854,20
PRIMER_RIGHT_28=1626,20
PRIMER_LEFT_28_TM=59.415
PRIMER_RIGHT_28_TM=60.567
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=60.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=9.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=773
PRIMER_PAIR_29_PENALTY=1.178783
PRIMER_LEFT_29_PENALTY=0.351810
PRIMER_RIGHT_29_PENALTY=0.826973
PRIMER_LEFT_29_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_29_SEQUENCE=TCTGGCAACGCTAACTTGGT
PRIMER_LEFT_29=840,20
PRIMER_RIGHT_29=1516,20
PRIMER_LEFT_29_TM=59.648
PRIMER_RIGHT_29_TM=60.827
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=677
PRIMER_PAIR_30_PENALTY=1.204535
PRIMER_LEFT_30_PENALTY=0.159749
PRIMER_RIGHT_30_PENALTY=1.044787
PRIMER_LEFT_30_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_30_SEQUENCE=TCTTTTGCGACTTCACTCGTT
PRIMER_LEFT_30=810,20
PRIMER_RIGHT_30=1672,21
PRIMER_LEFT_30_TM=60.160
PRIMER_RIGHT_30_TM=60.045
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=5.9000
PRIMER_RIGHT_30_END_STABILITY=8.4000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=863
PRIMER_PAIR_31_PENALTY=1.226425
PRIMER_LEFT_31_PENALTY=0.159749
PRIMER_RIGHT_31_PENALTY=1.066676
PRIMER_LEFT_31_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_31_SEQUENCE=AAGCACTCAGCAGTACGGAAA
PRIMER_LEFT_31=810,20
PRIMER_RIGHT_31=1591,21
PRIMER_LEFT_31_TM=60.160
PRIMER_RIGHT_31_TM=60.067
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.9000
PRIMER_RIGHT_31_END_STABILITY=8.5000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=782
PRIMER_PAIR_32_PENALTY=1.236698
PRIMER_LEFT_32_PENALTY=0.159749
PRIMER_RIGHT_32_PENALTY=1.076949
PRIMER_LEFT_32_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_32_SEQUENCE=CTCAGCAGTACGGAAACCAA
PRIMER_LEFT_32=810,20
PRIMER_RIGHT_32=1586,20
PRIMER_LEFT_32_TM=60.160
PRIMER_RIGHT_32_TM=58.923
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=5.9000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=777
PRIMER_PAIR_33_PENALTY=1.252182
PRIMER_LEFT_33_PENALTY=1.096820
PRIMER_RIGHT_33_PENALTY=0.155362
PRIMER_LEFT_33_SEQUENCE=GCTTTGCTATTGCTGTTGGTC
PRIMER_RIGHT_33_SEQUENCE=CCCATTCCATATTCTGCACC
PRIMER_LEFT_33=557,21
PRIMER_RIGHT_33=1378,20
PRIMER_LEFT_33_TM=59.903
PRIMER_RIGHT_33_TM=60.155
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=9.4000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=822
PRIMER_PAIR_34_PENALTY=1.254525
PRIMER_LEFT_34_PENALTY=0.351810
PRIMER_RIGHT_34_PENALTY=0.902715
PRIMER_LEFT_34_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_34_SEQUENCE=TCATCAGTTGGTCGTTTCCC
PRIMER_LEFT_34=840,20
PRIMER_RIGHT_34=1462,20
PRIMER_LEFT_34_TM=59.648
PRIMER_RIGHT_34_TM=60.903
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=623
PRIMER_PAIR_35_PENALTY=1.256534
PRIMER_LEFT_35_PENALTY=0.857208
PRIMER_RIGHT_35_PENALTY=0.399325
PRIMER_LEFT_35_SEQUENCE=GCAGAGACGTGGAGCTATGA
PRIMER_RIGHT_35_SEQUENCE=CCACCTCCAAAACCAGTGTC
PRIMER_LEFT_35=811,20
PRIMER_RIGHT_35=1611,20
PRIMER_LEFT_35_TM=59.143
PRIMER_RIGHT_35_TM=60.399
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=5.9000
PRIMER_RIGHT_35_END_STABILITY=6.1000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PAIR_35_PRODUCT_SIZE=801
PRIMER_PAIR_36_PENALTY=1.275987
PRIMER_LEFT_36_PENALTY=0.159749
PRIMER_RIGHT_36_PENALTY=1.116239
PRIMER_LEFT_36_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_36_SEQUENCE=TAAGTCCCACCTCCAAAACC
PRIMER_LEFT_36=810,20
PRIMER_RIGHT_36=1617,20
PRIMER_LEFT_36_TM=60.160
PRIMER_RIGHT_36_TM=58.884
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=808
PRIMER_PAIR_37_PENALTY=1.297083
PRIMER_LEFT_37_PENALTY=0.585216
PRIMER_RIGHT_37_PENALTY=0.711868
PRIMER_LEFT_37_SEQUENCE=TCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_37_SEQUENCE=TTCACAGCACCTAAGTCCCA
PRIMER_LEFT_37=854,20
PRIMER_RIGHT_37=1628,20
PRIMER_LEFT_37_TM=59.415
PRIMER_RIGHT_37_TM=59.288
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=775
PRIMER_PAIR_38_PENALTY=1.301386
PRIMER_LEFT_38_PENALTY=1.274264
PRIMER_RIGHT_38_PENALTY=0.027122
PRIMER_LEFT_38_SEQUENCE=CCTTGAAAGACACAAGGCATC
PRIMER_RIGHT_38_SEQUENCE=TTTTGCGACTTCACTCGTTG
PRIMER_LEFT_38=855,21
PRIMER_RIGHT_38=1670,20
PRIMER_LEFT_38_TM=59.726
PRIMER_RIGHT_38_TM=60.027
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.1000
PRIMER_RIGHT_38_END_STABILITY=8.7000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=816
PRIMER_PAIR_39_PENALTY=1.301386
PRIMER_LEFT_39_PENALTY=1.274264
PRIMER_RIGHT_39_PENALTY=0.027122
PRIMER_LEFT_39_SEQUENCE=ATCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_39_SEQUENCE=TTTTGCGACTTCACTCGTTG
PRIMER_LEFT_39=853,21
PRIMER_RIGHT_39=1670,20
PRIMER_LEFT_39_TM=59.726
PRIMER_RIGHT_39_TM=60.027
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=8.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=818
PRIMER_PAIR_40_PENALTY=1.309643
PRIMER_LEFT_40_PENALTY=0.159749
PRIMER_RIGHT_40_PENALTY=1.149895
PRIMER_LEFT_40_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_40_SEQUENCE=CTAAGTCCCACCTCCAAAACC
PRIMER_LEFT_40=810,20
PRIMER_RIGHT_40=1618,21
PRIMER_LEFT_40_TM=60.160
PRIMER_RIGHT_40_TM=59.850
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=52.381
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.9000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=809
PRIMER_PAIR_41_PENALTY=1.309643
PRIMER_LEFT_41_PENALTY=0.159749
PRIMER_RIGHT_41_PENALTY=1.149895
PRIMER_LEFT_41_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_41_SEQUENCE=CCTAAGTCCCACCTCCAAAAC
PRIMER_LEFT_41=810,20
PRIMER_RIGHT_41=1619,21
PRIMER_LEFT_41_TM=60.160
PRIMER_RIGHT_41_TM=59.850
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=5.9000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=810
PRIMER_PAIR_42_PENALTY=1.309643
PRIMER_LEFT_42_PENALTY=0.159749
PRIMER_RIGHT_42_PENALTY=1.149895
PRIMER_LEFT_42_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_42_SEQUENCE=ACCTAAGTCCCACCTCCAAAA
PRIMER_LEFT_42=810,20
PRIMER_RIGHT_42=1620,21
PRIMER_LEFT_42_TM=60.160
PRIMER_RIGHT_42_TM=59.850
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.9000
PRIMER_RIGHT_42_END_STABILITY=7.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=811
PRIMER_PAIR_43_PENALTY=1.315036
PRIMER_LEFT_43_PENALTY=1.274264
PRIMER_RIGHT_43_PENALTY=0.040772
PRIMER_LEFT_43_SEQUENCE=CCTTGAAAGACACAAGGCATC
PRIMER_RIGHT_43_SEQUENCE=CACCTAAGTCCCACCTCCAA
PRIMER_LEFT_43=855,21
PRIMER_RIGHT_43=1621,20
PRIMER_LEFT_43_TM=59.726
PRIMER_RIGHT_43_TM=59.959
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.1000
PRIMER_RIGHT_43_END_STABILITY=8.5000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=767
PRIMER_PAIR_44_PENALTY=1.315036
PRIMER_LEFT_44_PENALTY=1.274264
PRIMER_RIGHT_44_PENALTY=0.040772
PRIMER_LEFT_44_SEQUENCE=ATCCTTGAAAGACACAAGGCA
PRIMER_RIGHT_44_SEQUENCE=CACCTAAGTCCCACCTCCAA
PRIMER_LEFT_44=853,21
PRIMER_RIGHT_44=1621,20
PRIMER_LEFT_44_TM=59.726
PRIMER_RIGHT_44_TM=59.959
PRIMER_LEFT_44_GC_PERCENT=42.857
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.7000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=769
PRIMER_PAIR_45_PENALTY=1.327326
PRIMER_LEFT_45_PENALTY=0.351810
PRIMER_RIGHT_45_PENALTY=0.975516
PRIMER_LEFT_45_SEQUENCE=TGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_45_SEQUENCE=CCTAAGTCCCACCTCCAAAA
PRIMER_LEFT_45=840,20
PRIMER_RIGHT_45=1619,20
PRIMER_LEFT_45_TM=59.648
PRIMER_RIGHT_45_TM=59.024
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.5000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=780
PRIMER_PAIR_46_PENALTY=1.331543
PRIMER_LEFT_46_PENALTY=1.052696
PRIMER_RIGHT_46_PENALTY=0.278847
PRIMER_LEFT_46_SEQUENCE=ATGGCAGAATGTGAATCCTTG
PRIMER_RIGHT_46_SEQUENCE=CAGCACCTAAGTCCCACCTC
PRIMER_LEFT_46=839,21
PRIMER_RIGHT_46=1624,20
PRIMER_LEFT_46_TM=59.947
PRIMER_RIGHT_46_TM=59.721
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=60.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=786
PRIMER_PAIR_47_PENALTY=1.371326
PRIMER_LEFT_47_PENALTY=0.159749
PRIMER_RIGHT_47_PENALTY=1.211578
PRIMER_LEFT_47_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_47_SEQUENCE=CAGCAGTACGGAAACCAACTC
PRIMER_LEFT_47=810,20
PRIMER_RIGHT_47=1584,21
PRIMER_LEFT_47_TM=60.160
PRIMER_RIGHT_47_TM=59.788
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=52.381
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=5.9000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=775
PRIMER_PAIR_48_PENALTY=1.371326
PRIMER_LEFT_48_PENALTY=0.159749
PRIMER_RIGHT_48_PENALTY=1.211578
PRIMER_LEFT_48_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_48_SEQUENCE=TCAGCAGTACGGAAACCAACT
PRIMER_LEFT_48=810,20
PRIMER_RIGHT_48=1585,21
PRIMER_LEFT_48_TM=60.160
PRIMER_RIGHT_48_TM=59.788
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=5.9000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=776
PRIMER_PAIR_49_PENALTY=1.371326
PRIMER_LEFT_49_PENALTY=0.159749
PRIMER_RIGHT_49_PENALTY=1.211578
PRIMER_LEFT_49_SEQUENCE=TGCAGAGACGTGGAGCTATG
PRIMER_RIGHT_49_SEQUENCE=CTCAGCAGTACGGAAACCAAC
PRIMER_LEFT_49=810,20
PRIMER_RIGHT_49=1586,21
PRIMER_LEFT_49_TM=60.160
PRIMER_RIGHT_49_TM=59.788
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.9000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=777
=
SEQUENCE_ID=MAGIC.446_3_22516522_22516597_rsch-4_sf-2_4882177_51595_TRUE
SEQUENCE_TARGET=950,175
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_9173842_9173843_can-0_hi-0_4472_187974_TRUE
SEQUENCE_TARGET=950,101
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGAGTNTANNNNNNNNGTATGNGTGTGACTGCAAGTCCTTGCTACTATNNANTAAGCAGTACATATGTTTTTGGGTAACGATTATTTAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAAGAAAAAGTGCATATAAGATTTCATTAAGAAAATTAAAGCANAAAATAGATTATGNATGTCATGATGTTAGCAATGACATAGCATTCAAAATGATGTTAGCAATGACATTGCACACANAAACAAATGTGTGCTTTCAATATAANGGTTNAAAAATTACAAAAATGTTGGTTNTANTTNNNTNNNNNCANNANNTTNNNNTTNNTNAANNNNTNNNNACTANNGAGTTAGGCTTTTANACGTCANTTTANCCAAANNATTNCAGAATCACTTTAACCTGGTANANTTTCCTTTCACCANNTTAATTTANTTTTNNTATTTCNTNTAAATATGACNTTNNNNNNNNNNNNNNTNAAATTGCCTTTTNTAAAACNTAATTTGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGCAAGTTTATTAACGANTNANCTCANGCAAAAAGTATAAAAGATTTGNATTGNTTATGTANGTGTGTNTATATCAAACAGGTCGCATTCAAAAGTTCGAAATTCTTATATATTGGAAACAGGTAAATACTAAATAGTATTGTAATCNGATTCANNAAATTNACNNATNGTATTGTTTATCACGNTNACACTTTTGGGTGGCTATTTTATCCATCCGATTCTTATTTCCACTCTAAATAAGTNTANTGTCCAACGTTACCGTCTCCACCACTACCACTATGNNATCNTTTTCTCTTATANAAATATTATTAGGTAACAACTATTAATAAACAAAGCTATATACTCTATTTTTCTATATAAGGCGTGAGTTCTCCACAAGCCTCCTCATCAATCAANATCAAGTAAGCATCANTACTTNGTACTAAATCCAAACCATGNCTTCCAACGTTCAGCTTTCAGAAACAAACAGCCCAAGGAATCAGAAAANCCGNCCGAGAGCAGAGAAGGAGGAAGTGGACTACATGCAGAGAGCTCAGTGGCTAAGAGCTGCCTTGCTTGGAGCNAACGATGGTCTGGTCACAGTCGCTTCGCTAATGATGGGTGTTGGTTCTATCAAAGAAGACGTCAAAGCAATGCTTCTCGTTGGTTTTGCTGGCCTTGTNGCGGGTGCTTGTAGTATGGCTATTGGAGAGTTTGTGTCTGTGTGCACCCAAAGGGATATTGAAACTGCACAGATGAAGAGAGCTATTGANNATAAGACATCACTATCAGC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_1_12673470_12673549_kn-0_no-0_8797_77222_TRUE
SEQUENCE_TARGET=950,179
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTGCATCTCAACNTCTTATATTTTTTGTTGGTATTTNTATTTTGTNANNTTCANAAAAATTTATTTTNTCANNAATCTAAACTAATAAAATTACANTCTNTTTNTTTATANATNATTTTNTNTNNNNNNNNNNNNNNNNNCAATTATAANTTTNNNNNNNNNNNANATNTCTTTTATAGTANTNTTNNTAGAGAANNNNNNNNNNNNNNNNNNNNNNCATNTNNACATCTAATTAATTGANTGAACCATNNNNNTATTTATAATNNTNANNTTTGTTGGATTCTTATNATATTGATCTTAGCTGGATTCTCANGAGATTAAACAATTCAAGTTTTTAATGATAAAACATGTNTTATACATATTTGTTGAAGAAATGTTAACTAGCATTCCAAAAAGANNANNNNANNNNGTGTTGNATTCAATANTATTACTTTGCACNAAAANAAANNNAAAAAGGATTCAANNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_1747381_1747415_edi-0_sf-2_73538_6954_TRUE
SEQUENCE_TARGET=950,134
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTCTNGTTATATTTTNTTGCTTNGACGATTTTGTGAATGANNGTAGAAGAATANNAATATGAGAATTNTCAATGTTTCCN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_16183522_16183744_ct-1_hi-0_361772_114329_TRUE
SEQUENCE_TARGET=950,322
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAANAGNNCTAAATCTATAGGNAAAACATACNAAATCACATTGTANNNCTNTCCNTNTTCTNATAGTNTTANTGTAATATTTAAGTAATNNNNNNTNNNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCGAACCAACCTAAANTAAAATGAACTAANTNNANCNANANNAANCACANTNCAAAANAAAGNAATTNTNNTNGAGNTNNCNNNANNTTTTTNTNAAAACTAAATNAACCAAACCAANCCNANAANNAANNCNAATTGCCATCCCCTNNTAGAGATATTCAATAGNNGCATCCCCTANTTTCAAAACTGTCCAAACGTTTTATANATTTGAATTCTCAAACAAAGGAAATTTTGAAAANTATTTTGTNAAATATTCATGAGCTTGCATACAAGTAAAATCTATCGAGNTCACCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNGNGATGCTTCTTAGCACTTTNATTNTNTTNAAAATCTATGNTTNGGCNTAACNNGNGAATAGTTTNAATGANTCNNNAGTGTTGTTTGACAGTGAANNNAACATAGCACGATTAGACAACACTCAAGTNAATGAAGNNNTTNTCTTGTTCATATTTTGGTATTTTTNAGGNTTTTGAATCNNTATTTCTAACCCTANTTTNAGTTTCTANATANATTATGTTTNNAGGTTNTNAATANNGNNNNNNNNGTCTTTAAGNTNTTTTCANATAAAGGNGATNAAAAGCATTNAAAANNNTATGAANAGTCTTTNNANTTTTTANGATANANNTAANAAAGANANAACNGATTTNAAATTANANNAAAATNNACAAANGATTATNNGACNTANAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAANGNANNGNAATNGNCTTAAATATCATNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAAGTATGAAGNACNATGNGTATGAGAAGATTCCANCTTGAGTNTTTTCACTTTTGTATGANAACCTCATNAAAATCATATTGANTAACTGTGTGATCATATACACTCAGTAACTCNCATTTACCTTNAGAAAANTTAGNTTTCTCTANCTANNNNNNNNNNNNNNNNNNNNNNNNNNTTTNNCTTNANNGNNATAATTAAATAANCTGTTGTNTTACGTANTANGAGTGGATAAAAATAAAGCAACCAANCGATCTTTCTTNACAAAAANNNNNNNNNAAATAAAACGAGTACAAAATTTAACAAGAAATCATTTCATGTTGGCCNATCANCNNTTTTCTTTTACCTCGNGTATNTAGAACTTGTGCAACCCAACTCCTAATCGGTATACNAATTTTGGTCACACCCTCTATAANATTTGGAGAANATCCAATAGATCCAGTTTTTACACTGTCTTTCTTACATAAATNTCAGACTNAAATCTCTTTTTCGTTTTCAGAAGTAACATACCGATATAATATGCCGCATACCAACTTTTTTGATCAAATCATNTCTTTGCTCNTTTTTGTCCAAT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17964421_17965032_po-0_edi-0_18854_16991_TRUE
SEQUENCE_TARGET=950,711
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTCTTTTATAACAATTTTAGTTTTAATTAGNNAAANNNGACAAGATTTTGAAGGTTTAATAAGANAANTTANACTTCNTTTTAAATATTCATGATCAAATTTACAGCNNNNNNNNNTTTTANNNNTGANATCAAACCACAAACGCANNATTAATTTTTACATTTCCTAACTACACAAAATTTTAAAACTCAGCCTAAAACTGTTCTACNATTTTTAAGTGTTTGATANTTCTCNACTNGNGACGCNGAAGTTAAAAAANACTAAGANAAAATATATGAATGGAGANCGAANNGTNCAGTGCANTGNGATTCCAATAGTAACAAACATGATTANGGAGTATGACATAATCANAAAATATNAAATGTCACAAAGTTTGNTAGNCTTTGCNGCAAAATGGAAATGTCCGGGCATTGGACGGTAGGCCCAGACTAGACTTTGTGGCAAAATGGAAATGTCCAGGCGTTGGACCGTAGGCCCAGACGGNCAGACTCATAGTACCACATTTTACATGTAGNNTATAAATAGGTCTTNTAATGGAGTTTTGAGAANGTGGAAAACCTAGCTTAATGGCTACANAGACATCAAATTTCGTTTCTCTTNGCGTATCTTTGTTTATTCTTCTCTTATTTATCTCATCTCAAGTTGCTATCGCNGACGCCAANCATCTCCAACGTAAGTGTTTACACACTAACACACATAAGGCCTCTTNGCTTTTTCTAACACTCGCATGAACATAAGCTTGGTTACTGTTTAGNGATTTAACAATATACATAAGTTTGGCTNTGTATTCAATGTTCATTGATGAAANNTTTNTTATTGTATGAAACATAAAATGAAAGTTTATATCTTNNNNTGTCTCAACGTCAATTTNTNTNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGTCTNNGCTCNATCATGAATTAAATATTGTTTAGAATATCCAACATTAATTTTGAATCTAAAATATTAATATTCATAATTTTNAATTTTTANCTATGTCAAACAAAGCATATTTAAAAACTAAANTTTATAANNNNNNNTTGTAAATCAATAGAATTNAGAAGGAAGTTGCNGNTAGTACGACGATCAAGAAGTCAAAGANGTCGTCAATACAATCCACCGACGCTGCNNNTGCCGCCNCCACCACCACCGCCNCTGCCCCAGATGCCGAGTGCAGCCANACCTNCCCCAATGCCGCAGTTGNCACCACTGCAGCCGAAAATGCATGTATCANCACTGCAGCCACAAATGCTCTATCCACCTCCTTCGTTGCCGTATGCTTCATCGCCGACGGTATGAAAACAATAATTCCATTATTANAAAAATAGTTTTCAAATNATAAGATCGANTTTANNNNNNTATATACGATGATNACTANCTATAAAATCAATAAAANTTATAATTTATTATTATCGACACNAANNNTTTTNNGGAAATTACACATTCAAATTCTAANNTTNAAGAATATTTTATAAACCTAAANTTTTATGATATTTTCAGAGTTTTGTGTANTTTTAGAAAACAAAAGCACANAAAATGTGGTACTNTNCGAACTGAATTTTTTTGGTAAATTTATNTTCTTNTAAGAATCATAANTATANGTTTATCAATATATGCTATATTGNNNTCATGCAGTCTACATAACGAAGGAGAAAGATGACGCTGGTGGTAGTCGCATAACGAAGAAGGAAGCTGATGCTANTATGAGATAGCTTCAGTCCTTCATTTATCAAGGAAANAATAAATCTTTTTACNATATATNTTTTTTTTTTTNACGTATGTTGATATGAATCTTGCACTATTAATAACCATATCTTAGTCAATNTGATNNNNNATTTGTAATAATTGCTAAGATATATNATAATCCCAATANCTTTTNATNTTTTTGTTCCATGGATGATGGGAGTAATGACTATCTAGTATANAAGGTGTTATGTTGTTATTGTGATTNCTTTGTTAGCTAGATTTCTACTGCNTTTTTGTTTTTCTTTTGTCAAAAGATTTCTACTGCCTTTATTAAAGAAGTNGAATTATATATAGAGGACATCGACCAAAATATATATTCATTTAAGTTTTATATTATCTTGGATNGTTGATTTATGATTATATATAATTTTACTCAATATTAG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.707488
PRIMER_LEFT_0_PENALTY=1.401339
PRIMER_RIGHT_0_PENALTY=0.306150
PRIMER_LEFT_0_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_0_SEQUENCE=GCAACGAAGGAGGTGGATAG
PRIMER_LEFT_0=883,20
PRIMER_RIGHT_0=1754,20
PRIMER_LEFT_0_TM=58.599
PRIMER_RIGHT_0_TM=59.694
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=5.6000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=872
PRIMER_PAIR_1_PENALTY=1.754462
PRIMER_LEFT_1_PENALTY=1.401339
PRIMER_RIGHT_1_PENALTY=0.353123
PRIMER_LEFT_1_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_1_SEQUENCE=CGAAGGAGGTGGATAGAGCA
PRIMER_LEFT_1=883,20
PRIMER_RIGHT_1=1750,20
PRIMER_LEFT_1_TM=58.599
PRIMER_RIGHT_1_TM=60.353
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=868
PRIMER_PAIR_2_PENALTY=2.105303
PRIMER_LEFT_2_PENALTY=1.799154
PRIMER_RIGHT_2_PENALTY=0.306150
PRIMER_LEFT_2_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_2_SEQUENCE=GCAACGAAGGAGGTGGATAG
PRIMER_LEFT_2=882,20
PRIMER_RIGHT_2=1754,20
PRIMER_LEFT_2_TM=58.201
PRIMER_RIGHT_2_TM=59.694
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=873
PRIMER_PAIR_3_PENALTY=2.152277
PRIMER_LEFT_3_PENALTY=1.799154
PRIMER_RIGHT_3_PENALTY=0.353123
PRIMER_LEFT_3_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_3_SEQUENCE=CGAAGGAGGTGGATAGAGCA
PRIMER_LEFT_3=882,20
PRIMER_RIGHT_3=1750,20
PRIMER_LEFT_3_TM=58.201
PRIMER_RIGHT_3_TM=60.353
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=869
PRIMER_PAIR_4_PENALTY=2.261179
PRIMER_LEFT_4_PENALTY=1.401339
PRIMER_RIGHT_4_PENALTY=0.859840
PRIMER_LEFT_4_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_4_SEQUENCE=TGATACATGCATTTTCGGCT
PRIMER_LEFT_4=883,20
PRIMER_RIGHT_4=1714,20
PRIMER_LEFT_4_TM=58.599
PRIMER_RIGHT_4_TM=59.140
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=832
PRIMER_PAIR_5_PENALTY=2.954841
PRIMER_LEFT_5_PENALTY=1.401339
PRIMER_RIGHT_5_PENALTY=1.553502
PRIMER_LEFT_5_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_5_SEQUENCE=ATGAAGCATACGGCAACGAA
PRIMER_LEFT_5=883,20
PRIMER_RIGHT_5=1766,20
PRIMER_LEFT_5_TM=58.599
PRIMER_RIGHT_5_TM=61.554
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=884
PRIMER_PAIR_6_PENALTY=3.075779
PRIMER_LEFT_6_PENALTY=1.401339
PRIMER_RIGHT_6_PENALTY=1.674441
PRIMER_LEFT_6_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_6_SEQUENCE=GGATAGAGCATTTGTGGCTG
PRIMER_LEFT_6=883,20
PRIMER_RIGHT_6=1740,20
PRIMER_LEFT_6_TM=58.599
PRIMER_RIGHT_6_TM=58.326
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=858
PRIMER_PAIR_7_PENALTY=3.075779
PRIMER_LEFT_7_PENALTY=1.401339
PRIMER_RIGHT_7_PENALTY=1.674441
PRIMER_LEFT_7_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_7_SEQUENCE=TGGATAGAGCATTTGTGGCT
PRIMER_LEFT_7=883,20
PRIMER_RIGHT_7=1741,20
PRIMER_LEFT_7_TM=58.599
PRIMER_RIGHT_7_TM=58.326
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=859
PRIMER_PAIR_8_PENALTY=3.188973
PRIMER_LEFT_8_PENALTY=1.401339
PRIMER_RIGHT_8_PENALTY=1.787634
PRIMER_LEFT_8_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_8_SEQUENCE=GTGGATAGAGCATTTGTGGCT
PRIMER_LEFT_8=883,20
PRIMER_RIGHT_8=1742,21
PRIMER_LEFT_8_TM=58.599
PRIMER_RIGHT_8_TM=59.212
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=860
PRIMER_PAIR_9_PENALTY=3.335216
PRIMER_LEFT_9_PENALTY=1.401339
PRIMER_RIGHT_9_PENALTY=1.933877
PRIMER_LEFT_9_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_9_SEQUENCE=AGGTGGATAGAGCATTTGTGG
PRIMER_LEFT_9=883,20
PRIMER_RIGHT_9=1744,21
PRIMER_LEFT_9_TM=58.599
PRIMER_RIGHT_9_TM=59.066
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=862
PRIMER_PAIR_10_PENALTY=3.352656
PRIMER_LEFT_10_PENALTY=1.799154
PRIMER_RIGHT_10_PENALTY=1.553502
PRIMER_LEFT_10_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_10_SEQUENCE=ATGAAGCATACGGCAACGAA
PRIMER_LEFT_10=882,20
PRIMER_RIGHT_10=1766,20
PRIMER_LEFT_10_TM=58.201
PRIMER_RIGHT_10_TM=61.554
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=885
PRIMER_PAIR_11_PENALTY=3.382077
PRIMER_LEFT_11_PENALTY=1.401339
PRIMER_RIGHT_11_PENALTY=1.980738
PRIMER_LEFT_11_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_11_SEQUENCE=GGTGGATAGAGCATTTGTGG
PRIMER_LEFT_11=883,20
PRIMER_RIGHT_11=1743,20
PRIMER_LEFT_11_TM=58.599
PRIMER_RIGHT_11_TM=58.019
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=861
PRIMER_PAIR_12_PENALTY=3.473594
PRIMER_LEFT_12_PENALTY=1.799154
PRIMER_RIGHT_12_PENALTY=1.674441
PRIMER_LEFT_12_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_12_SEQUENCE=GGATAGAGCATTTGTGGCTG
PRIMER_LEFT_12=882,20
PRIMER_RIGHT_12=1740,20
PRIMER_LEFT_12_TM=58.201
PRIMER_RIGHT_12_TM=58.326
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=859
PRIMER_PAIR_13_PENALTY=3.473594
PRIMER_LEFT_13_PENALTY=1.799154
PRIMER_RIGHT_13_PENALTY=1.674441
PRIMER_LEFT_13_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_13_SEQUENCE=TGGATAGAGCATTTGTGGCT
PRIMER_LEFT_13=882,20
PRIMER_RIGHT_13=1741,20
PRIMER_LEFT_13_TM=58.201
PRIMER_RIGHT_13_TM=58.326
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=860
PRIMER_PAIR_14_PENALTY=3.515854
PRIMER_LEFT_14_PENALTY=1.401339
PRIMER_RIGHT_14_PENALTY=2.114515
PRIMER_LEFT_14_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_14_SEQUENCE=GATGAAGCATACGGCAACGA
PRIMER_LEFT_14=883,20
PRIMER_RIGHT_14=1767,20
PRIMER_LEFT_14_TM=58.599
PRIMER_RIGHT_14_TM=62.115
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=885
PRIMER_PAIR_15_PENALTY=3.558608
PRIMER_LEFT_15_PENALTY=1.401339
PRIMER_RIGHT_15_PENALTY=2.157269
PRIMER_LEFT_15_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_15_SEQUENCE=ACGAAGGAGGTGGATAGAGCA
PRIMER_LEFT_15=883,20
PRIMER_RIGHT_15=1751,21
PRIMER_LEFT_15_TM=58.599
PRIMER_RIGHT_15_TM=61.157
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=52.381
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=869
PRIMER_PAIR_16_PENALTY=3.573711
PRIMER_LEFT_16_PENALTY=1.401339
PRIMER_RIGHT_16_PENALTY=2.172373
PRIMER_LEFT_16_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_16_SEQUENCE=TGGATAGAGCATTTGTGGCTG
PRIMER_LEFT_16=883,20
PRIMER_RIGHT_16=1741,21
PRIMER_LEFT_16_TM=58.599
PRIMER_RIGHT_16_TM=61.172
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=859
PRIMER_PAIR_17_PENALTY=3.586788
PRIMER_LEFT_17_PENALTY=1.799154
PRIMER_RIGHT_17_PENALTY=1.787634
PRIMER_LEFT_17_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_17_SEQUENCE=GTGGATAGAGCATTTGTGGCT
PRIMER_LEFT_17=882,20
PRIMER_RIGHT_17=1742,21
PRIMER_LEFT_17_TM=58.201
PRIMER_RIGHT_17_TM=59.212
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=861
PRIMER_PAIR_18_PENALTY=3.600432
PRIMER_LEFT_18_PENALTY=1.401339
PRIMER_RIGHT_18_PENALTY=2.199093
PRIMER_LEFT_18_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_18_SEQUENCE=CAACGAAGGAGGTGGATAGAG
PRIMER_LEFT_18=883,20
PRIMER_RIGHT_18=1753,21
PRIMER_LEFT_18_TM=58.599
PRIMER_RIGHT_18_TM=58.801
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=5.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=871
PRIMER_PAIR_19_PENALTY=3.651298
PRIMER_LEFT_19_PENALTY=1.401339
PRIMER_RIGHT_19_PENALTY=2.249959
PRIMER_LEFT_19_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_19_SEQUENCE=TGAAGCATACGGCAACGAAG
PRIMER_LEFT_19=883,20
PRIMER_RIGHT_19=1765,20
PRIMER_LEFT_19_TM=58.599
PRIMER_RIGHT_19_TM=62.250
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=8.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=883
PRIMER_PAIR_20_PENALTY=3.662416
PRIMER_LEFT_20_PENALTY=1.401339
PRIMER_RIGHT_20_PENALTY=2.261077
PRIMER_LEFT_20_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_20_SEQUENCE=CAACGAAGGAGGTGGATAGA
PRIMER_LEFT_20=883,20
PRIMER_RIGHT_20=1753,20
PRIMER_LEFT_20_TM=58.599
PRIMER_RIGHT_20_TM=57.739
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=5.6000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=871
PRIMER_PAIR_21_PENALTY=3.733031
PRIMER_LEFT_21_PENALTY=1.799154
PRIMER_RIGHT_21_PENALTY=1.933877
PRIMER_LEFT_21_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_21_SEQUENCE=AGGTGGATAGAGCATTTGTGG
PRIMER_LEFT_21=882,20
PRIMER_RIGHT_21=1744,21
PRIMER_LEFT_21_TM=58.201
PRIMER_RIGHT_21_TM=59.066
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=863
PRIMER_PAIR_22_PENALTY=3.733972
PRIMER_LEFT_22_PENALTY=1.401339
PRIMER_RIGHT_22_PENALTY=2.332633
PRIMER_LEFT_22_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_22_SEQUENCE=ATACGGCAACGAAGGAGGTG
PRIMER_LEFT_22=883,20
PRIMER_RIGHT_22=1759,20
PRIMER_LEFT_22_TM=58.599
PRIMER_RIGHT_22_TM=62.333
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.5000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=877
PRIMER_PAIR_23_PENALTY=3.733972
PRIMER_LEFT_23_PENALTY=1.401339
PRIMER_RIGHT_23_PENALTY=2.332633
PRIMER_LEFT_23_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_23_SEQUENCE=CATACGGCAACGAAGGAGGT
PRIMER_LEFT_23=883,20
PRIMER_RIGHT_23=1760,20
PRIMER_LEFT_23_TM=58.599
PRIMER_RIGHT_23_TM=62.333
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=7.6000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=878
PRIMER_PAIR_24_PENALTY=3.761168
PRIMER_LEFT_24_PENALTY=3.455019
PRIMER_RIGHT_24_PENALTY=0.306150
PRIMER_LEFT_24_SEQUENCE=ATTGGACGGTAGGCCCAGAC
PRIMER_RIGHT_24_SEQUENCE=GCAACGAAGGAGGTGGATAG
PRIMER_LEFT_24=861,20
PRIMER_RIGHT_24=1754,20
PRIMER_LEFT_24_TM=63.455
PRIMER_RIGHT_24_TM=59.694
PRIMER_LEFT_24_GC_PERCENT=60.000
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=5.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=894
PRIMER_PAIR_25_PENALTY=3.779892
PRIMER_LEFT_25_PENALTY=1.799154
PRIMER_RIGHT_25_PENALTY=1.980738
PRIMER_LEFT_25_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_25_SEQUENCE=GGTGGATAGAGCATTTGTGG
PRIMER_LEFT_25=882,20
PRIMER_RIGHT_25=1743,20
PRIMER_LEFT_25_TM=58.201
PRIMER_RIGHT_25_TM=58.019
PRIMER_LEFT_25_GC_PERCENT=40.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.1000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=862
PRIMER_PAIR_26_PENALTY=3.808142
PRIMER_LEFT_26_PENALTY=3.455019
PRIMER_RIGHT_26_PENALTY=0.353123
PRIMER_LEFT_26_SEQUENCE=ATTGGACGGTAGGCCCAGAC
PRIMER_RIGHT_26_SEQUENCE=CGAAGGAGGTGGATAGAGCA
PRIMER_LEFT_26=861,20
PRIMER_RIGHT_26=1750,20
PRIMER_LEFT_26_TM=63.455
PRIMER_RIGHT_26_TM=60.353
PRIMER_LEFT_26_GC_PERCENT=60.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=890
PRIMER_PAIR_27_PENALTY=3.913669
PRIMER_LEFT_27_PENALTY=1.799154
PRIMER_RIGHT_27_PENALTY=2.114515
PRIMER_LEFT_27_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_27_SEQUENCE=GATGAAGCATACGGCAACGA
PRIMER_LEFT_27=882,20
PRIMER_RIGHT_27=1767,20
PRIMER_LEFT_27_TM=58.201
PRIMER_RIGHT_27_TM=62.115
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.1000
PRIMER_RIGHT_27_END_STABILITY=8.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=886
PRIMER_PAIR_28_PENALTY=3.919138
PRIMER_LEFT_28_PENALTY=1.401339
PRIMER_RIGHT_28_PENALTY=2.517800
PRIMER_LEFT_28_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_28_SEQUENCE=GCAACGAAGGAGGTGGATAGA
PRIMER_LEFT_28=883,20
PRIMER_RIGHT_28=1754,21
PRIMER_LEFT_28_TM=58.599
PRIMER_RIGHT_28_TM=61.518
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=52.381
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=5.6000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=872
PRIMER_PAIR_29_PENALTY=3.956423
PRIMER_LEFT_29_PENALTY=1.799154
PRIMER_RIGHT_29_PENALTY=2.157269
PRIMER_LEFT_29_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_29_SEQUENCE=ACGAAGGAGGTGGATAGAGCA
PRIMER_LEFT_29=882,20
PRIMER_RIGHT_29=1751,21
PRIMER_LEFT_29_TM=58.201
PRIMER_RIGHT_29_TM=61.157
PRIMER_LEFT_29_GC_PERCENT=40.000
PRIMER_RIGHT_29_GC_PERCENT=52.381
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.1000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=870
PRIMER_PAIR_30_PENALTY=3.971526
PRIMER_LEFT_30_PENALTY=1.799154
PRIMER_RIGHT_30_PENALTY=2.172373
PRIMER_LEFT_30_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_30_SEQUENCE=TGGATAGAGCATTTGTGGCTG
PRIMER_LEFT_30=882,20
PRIMER_RIGHT_30=1741,21
PRIMER_LEFT_30_TM=58.201
PRIMER_RIGHT_30_TM=61.172
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.1000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=860
PRIMER_PAIR_31_PENALTY=3.989719
PRIMER_LEFT_31_PENALTY=1.401339
PRIMER_RIGHT_31_PENALTY=2.588380
PRIMER_LEFT_31_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_31_SEQUENCE=AGCATACGGCAACGAAGGAG
PRIMER_LEFT_31=883,20
PRIMER_RIGHT_31=1762,20
PRIMER_LEFT_31_TM=58.599
PRIMER_RIGHT_31_TM=62.588
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=880
PRIMER_PAIR_32_PENALTY=3.998247
PRIMER_LEFT_32_PENALTY=1.799154
PRIMER_RIGHT_32_PENALTY=2.199093
PRIMER_LEFT_32_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_32_SEQUENCE=CAACGAAGGAGGTGGATAGAG
PRIMER_LEFT_32=882,20
PRIMER_RIGHT_32=1753,21
PRIMER_LEFT_32_TM=58.201
PRIMER_RIGHT_32_TM=58.801
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=52.381
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.1000
PRIMER_RIGHT_32_END_STABILITY=5.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=872
PRIMER_PAIR_33_PENALTY=4.049113
PRIMER_LEFT_33_PENALTY=1.799154
PRIMER_RIGHT_33_PENALTY=2.249959
PRIMER_LEFT_33_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_33_SEQUENCE=TGAAGCATACGGCAACGAAG
PRIMER_LEFT_33=882,20
PRIMER_RIGHT_33=1765,20
PRIMER_LEFT_33_TM=58.201
PRIMER_RIGHT_33_TM=62.250
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.1000
PRIMER_RIGHT_33_END_STABILITY=8.7000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=884
PRIMER_PAIR_34_PENALTY=4.060231
PRIMER_LEFT_34_PENALTY=1.799154
PRIMER_RIGHT_34_PENALTY=2.261077
PRIMER_LEFT_34_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_34_SEQUENCE=CAACGAAGGAGGTGGATAGA
PRIMER_LEFT_34=882,20
PRIMER_RIGHT_34=1753,20
PRIMER_LEFT_34_TM=58.201
PRIMER_RIGHT_34_TM=57.739
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=5.6000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=872
PRIMER_PAIR_35_PENALTY=4.131787
PRIMER_LEFT_35_PENALTY=1.799154
PRIMER_RIGHT_35_PENALTY=2.332633
PRIMER_LEFT_35_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_35_SEQUENCE=ATACGGCAACGAAGGAGGTG
PRIMER_LEFT_35=882,20
PRIMER_RIGHT_35=1759,20
PRIMER_LEFT_35_TM=58.201
PRIMER_RIGHT_35_TM=62.333
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=878
PRIMER_PAIR_36_PENALTY=4.131787
PRIMER_LEFT_36_PENALTY=1.799154
PRIMER_RIGHT_36_PENALTY=2.332633
PRIMER_LEFT_36_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_36_SEQUENCE=CATACGGCAACGAAGGAGGT
PRIMER_LEFT_36=882,20
PRIMER_RIGHT_36=1760,20
PRIMER_LEFT_36_TM=58.201
PRIMER_RIGHT_36_TM=62.333
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.1000
PRIMER_RIGHT_36_END_STABILITY=7.6000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=879
PRIMER_PAIR_37_PENALTY=4.267238
PRIMER_LEFT_37_PENALTY=1.401339
PRIMER_RIGHT_37_PENALTY=2.865899
PRIMER_LEFT_37_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_37_SEQUENCE=GGAGGTGGATAGAGCATTTGT
PRIMER_LEFT_37=883,20
PRIMER_RIGHT_37=1746,21
PRIMER_LEFT_37_TM=58.599
PRIMER_RIGHT_37_TM=58.134
PRIMER_LEFT_37_GC_PERCENT=40.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=864
PRIMER_PAIR_38_PENALTY=4.273904
PRIMER_LEFT_38_PENALTY=1.401339
PRIMER_RIGHT_38_PENALTY=2.872565
PRIMER_LEFT_38_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_38_SEQUENCE=AGGAGGTGGATAGAGCATTTGT
PRIMER_LEFT_38=883,20
PRIMER_RIGHT_38=1747,22
PRIMER_LEFT_38_TM=58.599
PRIMER_RIGHT_38_TM=59.127
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=45.455
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=865
PRIMER_PAIR_39_PENALTY=4.276371
PRIMER_LEFT_39_PENALTY=1.401339
PRIMER_RIGHT_39_PENALTY=2.875033
PRIMER_LEFT_39_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_39_SEQUENCE=GAGGTGGATAGAGCATTTGTGG
PRIMER_LEFT_39=883,20
PRIMER_RIGHT_39=1745,22
PRIMER_LEFT_39_TM=58.599
PRIMER_RIGHT_39_TM=60.875
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=863
PRIMER_PAIR_40_PENALTY=4.276371
PRIMER_LEFT_40_PENALTY=1.401339
PRIMER_RIGHT_40_PENALTY=2.875033
PRIMER_LEFT_40_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_40_SEQUENCE=GGAGGTGGATAGAGCATTTGTG
PRIMER_LEFT_40=883,20
PRIMER_RIGHT_40=1746,22
PRIMER_LEFT_40_TM=58.599
PRIMER_RIGHT_40_TM=60.875
PRIMER_LEFT_40_GC_PERCENT=40.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.5000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=864
PRIMER_PAIR_41_PENALTY=4.314859
PRIMER_LEFT_41_PENALTY=3.455019
PRIMER_RIGHT_41_PENALTY=0.859840
PRIMER_LEFT_41_SEQUENCE=ATTGGACGGTAGGCCCAGAC
PRIMER_RIGHT_41_SEQUENCE=TGATACATGCATTTTCGGCT
PRIMER_LEFT_41=861,20
PRIMER_RIGHT_41=1714,20
PRIMER_LEFT_41_TM=63.455
PRIMER_RIGHT_41_TM=59.140
PRIMER_LEFT_41_GC_PERCENT=60.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=11.4000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=854
PRIMER_PAIR_42_PENALTY=4.316953
PRIMER_LEFT_42_PENALTY=1.799154
PRIMER_RIGHT_42_PENALTY=2.517800
PRIMER_LEFT_42_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_42_SEQUENCE=GCAACGAAGGAGGTGGATAGA
PRIMER_LEFT_42=882,20
PRIMER_RIGHT_42=1754,21
PRIMER_LEFT_42_TM=58.201
PRIMER_RIGHT_42_TM=61.518
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.1000
PRIMER_RIGHT_42_END_STABILITY=5.6000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=873
PRIMER_PAIR_43_PENALTY=4.336816
PRIMER_LEFT_43_PENALTY=1.401339
PRIMER_RIGHT_43_PENALTY=2.935478
PRIMER_LEFT_43_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_43_SEQUENCE=AAGCATACGGCAACGAAGGA
PRIMER_LEFT_43=883,20
PRIMER_RIGHT_43=1763,20
PRIMER_LEFT_43_TM=58.599
PRIMER_RIGHT_43_TM=62.935
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=881
PRIMER_PAIR_44_PENALTY=4.336816
PRIMER_LEFT_44_PENALTY=1.401339
PRIMER_RIGHT_44_PENALTY=2.935478
PRIMER_LEFT_44_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_44_SEQUENCE=GAAGCATACGGCAACGAAGG
PRIMER_LEFT_44=883,20
PRIMER_RIGHT_44=1764,20
PRIMER_LEFT_44_TM=58.599
PRIMER_RIGHT_44_TM=62.935
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=882
PRIMER_PAIR_45_PENALTY=4.387534
PRIMER_LEFT_45_PENALTY=1.799154
PRIMER_RIGHT_45_PENALTY=2.588380
PRIMER_LEFT_45_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_45_SEQUENCE=AGCATACGGCAACGAAGGAG
PRIMER_LEFT_45=882,20
PRIMER_RIGHT_45=1762,20
PRIMER_LEFT_45_TM=58.201
PRIMER_RIGHT_45_TM=62.588
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.1000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=881
PRIMER_PAIR_46_PENALTY=4.663490
PRIMER_LEFT_46_PENALTY=1.401339
PRIMER_RIGHT_46_PENALTY=3.262151
PRIMER_LEFT_46_SEQUENCE=GACTTTGTGGCAAAATGGAA
PRIMER_RIGHT_46_SEQUENCE=GAAGGAGGTGGATAGAGCATTT
PRIMER_LEFT_46=883,20
PRIMER_RIGHT_46=1749,22
PRIMER_LEFT_46_TM=58.599
PRIMER_RIGHT_46_TM=58.738
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=45.455
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.2000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=2.00
PRIMER_PAIR_46_PRODUCT_SIZE=867
PRIMER_PAIR_47_PENALTY=4.665053
PRIMER_LEFT_47_PENALTY=1.799154
PRIMER_RIGHT_47_PENALTY=2.865899
PRIMER_LEFT_47_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_47_SEQUENCE=GGAGGTGGATAGAGCATTTGT
PRIMER_LEFT_47=882,20
PRIMER_RIGHT_47=1746,21
PRIMER_LEFT_47_TM=58.201
PRIMER_RIGHT_47_TM=58.134
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.1000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=2.00
PRIMER_PAIR_47_PRODUCT_SIZE=865
PRIMER_PAIR_48_PENALTY=4.671719
PRIMER_LEFT_48_PENALTY=1.799154
PRIMER_RIGHT_48_PENALTY=2.872565
PRIMER_LEFT_48_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_48_SEQUENCE=AGGAGGTGGATAGAGCATTTGT
PRIMER_LEFT_48=882,20
PRIMER_RIGHT_48=1747,22
PRIMER_LEFT_48_TM=58.201
PRIMER_RIGHT_48_TM=59.127
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=45.455
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.1000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=866
PRIMER_PAIR_49_PENALTY=4.674186
PRIMER_LEFT_49_PENALTY=1.799154
PRIMER_RIGHT_49_PENALTY=2.875033
PRIMER_LEFT_49_SEQUENCE=AGACTTTGTGGCAAAATGGA
PRIMER_RIGHT_49_SEQUENCE=GAGGTGGATAGAGCATTTGTGG
PRIMER_LEFT_49=882,20
PRIMER_RIGHT_49=1745,22
PRIMER_LEFT_49_TM=58.201
PRIMER_RIGHT_49_TM=60.875
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.1000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=864
=
SEQUENCE_ID=MAGIC.105_4_2584051_2584441_kn-0_tsu-0_4787_6019_TRUE
SEQUENCE_TARGET=950,490
SEQUENCE_TEMPLATE=GTTTGTCTCCCTCTATTTTTTTTNTCTTTTCGNTTCCATGCATCTACCTTTTCAATTTTCAATTCATGAGAGATACAATTTTCAATTGAAGTTTGGTTTGATTTTGCTTAAATGTGGGAAATTGATAATTGAATTGTATGTATATTGCAAGAGAAGTTTAGAAATTTTGTTTTCGGNTTTGTGTTCTTNTTTATGANTTGTGGTGTTTGAGTTTTGTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTNTNTTCTTTAATTGCTTTGTGATGATTCTTTNGTAGATGTGGAATAATNATTGGNTTCATTAGTGACGATATGNATTTGAAAATNTTAGNGAGCATTACTTGAGCANCTACATAATTTCTTGAGCAGCTACATNNTTNCNCNNNNTNGGGTTTTGGTTAGTCATAATGTTAGGGAGCANTTGGNTGTTTTAGTTTTGTTNTAAGTTTAGCTAGATTCTTTGTTGGAATANAGACAATTTGAAGNTTAAAGTGTTNNNGTTCATAACGNAATTATTGGATTCATCGTCNNTTGTGCTGTTTTNTGCAGTTGTAGTTATGACTTTTTCGGTTGTTTGTTGTGTAGTATTTGCATATTCGGTCTTTGATAANNNNCAGACAAATGTGTTCGNTCTCTGNNNNNTTTTTATCCCCTAAACTTTGATTTTNATGCATCAAANAANTGGTNTCTNTTGAGTTTNTGTACAATGGGTATGTNTNGTTTTGTNGTTGTCNATTCTGTTTCTATGGAGAAGACTNATCCAATTCTCANNNNNNGCTTCTTNTTTTGTCTTTCCNCTTCTTGATTGGATNAAATCCCAAGCCTTACGNNNCNATGAACCCATCTTNTNTTGACTCNGGTTTTTAGATTTAGCNTANGTTTATGAGAATCNANAAAAAAAGGTGGNGGTGGTNGGTTTTGTTNTTATNGAGGAGTTGAACTTGATTTGGNNAANAAGTCTTCNTTTTTCTTATTNATTAGTTACNAGNTTCCNTTTTCATCNANAANAGATTCATATCTTCACGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGAGAAACTAAAGTAGCNGTTCTTNTCGGATCTACATGTTANNTTTTACACNNTNTGGTCTCAAAACACCAAANATTTGGTTAATGAAGCTATATCGTGTTCATCNTCTAACNTGNTNAGTGTGTTGTCTATTACATTNAATANTTTGCTNNNNNNNNNGTGTGTACTNNNTTTTTTNANGNTTTTNATNTATATTACNTNNGTGGTTTANCTTCTTAATGNAAANCGANGAANGCATCANGAGTATGAGTTCANGTACTTTNAGATACAATTTATTTTTNTTTCTNATGANAGCTATTTACTAATAACTNTNNAGCTATTATCTCTNNTTTTNTTAANATATAAGTGATTGTTATGTGNANACATTGATAAGGAGTATCNNAAGNGCTNATNCNGANATGGTAGAGAGCCTNTTGTNGGAAAANTCAACAACTTCTNCAAAACANNATNNTNNAGAGAATCANGAAAGCTTNNCCANTAGAGTATAAANAAGTGAAGAGTGATCCTCNGTTTNCACACGTCTTTGCCATNTTTGAAANCNNTTTAGGANNNNNNNNNNNTACGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTNNNNNGATGANAANGGGTTTTGGAGNAAGTTNCTTAAGANANGGAACANTATNTNCATTAAGACNTTNATGGATAAGNNNTTATNAGAAGTTCANNNGTGGGATGAAGTNGACNNGGTAAGNTTTGTTTATCTNTGTGTGATTGNNGNATAGTGGTTGCTAGGGATGAGANNNAANATATCAAAGTAAAATACATCAAGTTGGTGATGGANCTTGAGAAGTTGAGAGCATATCNTTGGGNTNTACNTTCGTTNGNNNNNNNTAGTCAATTCCAAAAACNGCAATCTCCCANGATTTNTTCANCAANAATAAATCTCCACTCCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_24822501_24822591_oy-0_ler-0_236313_3037624_TRUE
SEQUENCE_TARGET=950,190
SEQUENCE_TEMPLATE=AGTTGATCTAAGATGACCAACATGCATTTCTTTAGCAATGTTGGGAGAGGAAAAATCAACTACAGCTCTCNTAACNGANAGAGTAGGNGCCCATGTATCAATCCCATCAACAANCATATTNTCAATACTCTGCAGATAGATTANAAAACAATTATGACAAACACAAAATTATTAACACAAGAACTAGAACATGACTATACCTTAGCCATCCACTTAGATGATAGNACAACATTAACAAATCCAGGACCAGCTATAGNGCATGANTCCACCATCTCAGAAGTAGGGAGACTCTGAATAAGAGCCTACATGAAAAAAATGAAACAGAATGAGNNNNNANTTANAAGAGANAAAAGCAATGAAATTCTNAAGACTAAACCAANCAACAAACCTGNCCAACAGCNGGAGGACCACGGAACTGCGTACCCTTTCCTTTAATTAGAGACCATAGTCCCATTGCATTGTTACTGCAGANAAACNATATGATGAATTACACAAACAGAAAACAACTTAATTGGTAGTTTAAGTTGGAAGAGTGANCACCATTGGTAATCTCCAAATTTGCCAGGCTCGATCAAGGGCTCAACATTAGGTTCATCAGGAACCGTTAACTTAAGNGACACATCGAAGAGCTTAGCGAGTTGGCGTCTTGGATTTCCGGTGAATTCTTTANTCTGTNAACATCATTCAANAAAAACAGTCATGAAACNGGAANGAAGANNNNNNNNNNNTCCTGGATNAAAAAACTATANTGTTACTTACAGCTACAATGGTTGCCATCGAAATTGAAGNANAAGACTGGTGAGAGCAGAGGAANNNNNNNNNNNNNNNACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTNCTATTGATGTNCATCAGTTTCAAATTNCTCNAAAAANGNATANAATTTTCATNTTTGTANTTGCGACGTNTTTNGGATTGAATGTANNNTTTGAAATNTTNNCATGTCCNTCAAACTATATTCTAATTACGANCNAGCTTGGTCCNGGTTTGGTTNTACANTANCNTTGTCACTCAAGAGACNACAATNTAAATGTNNAAAATTTACAGTTCAATGAAANAAAAAAAATNCGTNTCGCNNANAAGATAGGNAAAANGACAAGATGGANTTGTNTTTTGAAACACGNCTTATACATGCGATATTATTCAGAATTTATTGGATANAGAATGGCCAATGTTCGTNGATGTGGTNCTTCANGTNAGTGGATTGNCA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_3_1728604_1728606_po-0_wu-0_106449_135529_TRUE
SEQUENCE_TARGET=950,102
SEQUENCE_TEMPLATE=TCTACGTCNATCAAGGGTAAGCTCTGAAGAAGNTCCGGTNAGGCATCTTAGTCGTAGATGGAGAGCCACAACGGCGCAGAAGGTATATTCCNTAAAGCTCTACGACGCTCTTCAACGATCGCGGCGGAGCGCAACAGTCCGGGACACGGCCGACAAAGTCCTTGCGACGACCGCTCGTGGTGCAACTCGGTGGAGCCGAGCCATTTTGGTTAGTCGATTCGGAACGAGTCTGAGGCGGCGTAGGAATACCAAACCGGCGTCGGCATTAGCTGCGGCGATCAGGGGAAGTGGTGGAAGTGGGAGGAGGAGGAAGTTATCGGCGGTGGGAAATCGGGTCCGGGTTTTGGGTGGGTTGGTGCCGGGTTGCCGGAGAACGGCGTTACCGGAGCTTTTGGACGAGACAGCAGATTACATAGCGGCTTTGGAAATGCAAGTCCGAGCCATGACAGCTCTATCAAAGATTCTGTCGGAGCTTCAGCCGTCTACTAATCTCGGCTCGGCTTTATAGCCGAGTGTATAAGCCGATGAATTTGTTTCAATTAATTCTTTTACGATTTTATCGTGGGAATGTCTTATTGTTTTTAATGATTTGTTTGCCCTGGNAAAAAAAAANTGTCAAAATCATCACGAGTTGGACGAAAATACTATGTTTTTCTAAAAACCTTATAATAGGATTCGAAATATTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGACGGAGAAGGCATGTGCATGACGACCCTACGGTCCGACTCTTTTGCTCAAAGTGCGTCGCATGTGAGAGACCTCTTTATCTGAATTTAGTTTCTTCTCGAACANNNNNNNNNTTTTTTTTTNAAATCTTTAATTTGTAAGAAAATTAAATTCATAAAGAAGATACAATAAGTTAAAACGCCTTNCGTGTGTCCTACAACTTAACCAACTTTGTGAAACAAAATGAGTGCATATATCATACTTTGTCCTCACAAAAATGGATCAGGATTATCTTTAGTTATAGCTAAATTCGTACATGATGAATAATATTTAGACCAATNCCAGCCAACCAGTTTAGTTTGGTTTATGGTTCGCTAATTACTNAANNAAATCTGAAANTAGATAACATTTTTAAAGTTCTGACGATATGAATTTAAGTTGAATGTTTGTTAGCCTTTGAAGAAATGNTTAACGTACGTTTTGCGAAGTTAATTCTCAGTCAATTATCAACAAAAGAAAACACATATTTAACCTATGATATTTGGAGCAATAACATATGAATGTAAACAACATTAGGGCATTAATTAATTAGTAGATAATTATGAAAATCTACGTNCATNNNNNNANCGAGGACATGGCCGACGTGATACGTAGGAGCANAAATTCATATAGGCAACCAATTGTGGCTANCAAGTGGTATCCATCCCTTTAGTTTTTATGCAAACCATTAATTCATTTTAAGTGACAAAAGGAAAACACGGCTTTATATAGCTAGATTGCAAAATTATTTGCGATGAATATATCATGATTGATATAAGAAAAAACTAATATACGTA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.135201
PRIMER_LEFT_0_PENALTY=0.010545
PRIMER_RIGHT_0_PENALTY=0.124655
PRIMER_LEFT_0_SEQUENCE=GGAGCTTTTGGACGAGACAG
PRIMER_RIGHT_0_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_0=384,20
PRIMER_RIGHT_0=1233,20
PRIMER_LEFT_0_TM=59.989
PRIMER_RIGHT_0_TM=59.875
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=5.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=7.4000
PRIMER_PAIR_0_COMPL_ANY=6.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=850
PRIMER_PAIR_1_PENALTY=0.310598
PRIMER_LEFT_1_PENALTY=0.185942
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=GAAGTGGGAGGAGGAGGAAG
PRIMER_RIGHT_1_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_1=293,20
PRIMER_RIGHT_1=1233,20
PRIMER_LEFT_1_TM=60.186
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=941
PRIMER_PAIR_2_PENALTY=0.364512
PRIMER_LEFT_2_PENALTY=0.239857
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TCATCACGAGTTGGACGAAA
PRIMER_RIGHT_2_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_2=621,20
PRIMER_RIGHT_2=1233,20
PRIMER_LEFT_2_TM=60.240
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.0000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=613
PRIMER_PAIR_3_PENALTY=0.433377
PRIMER_LEFT_3_PENALTY=0.308722
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=CATCACGAGTTGGACGAAAA
PRIMER_RIGHT_3_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_3=622,20
PRIMER_RIGHT_3=1233,20
PRIMER_LEFT_3_TM=59.691
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=612
PRIMER_PAIR_4_PENALTY=0.751027
PRIMER_LEFT_4_PENALTY=0.626371
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=GCCGAGTGTATAAGCCGATG
PRIMER_RIGHT_4_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_4=507,20
PRIMER_RIGHT_4=1233,20
PRIMER_LEFT_4_TM=60.626
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=727
PRIMER_PAIR_5_PENALTY=0.963607
PRIMER_LEFT_5_PENALTY=0.838952
PRIMER_RIGHT_5_PENALTY=0.124655
PRIMER_LEFT_5_SEQUENCE=CTTTTGGACGAGACAGCAGA
PRIMER_RIGHT_5_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_5=388,20
PRIMER_RIGHT_5=1233,20
PRIMER_LEFT_5_TM=59.161
PRIMER_RIGHT_5_TM=59.875
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=846
PRIMER_PAIR_6_PENALTY=0.973980
PRIMER_LEFT_6_PENALTY=0.849325
PRIMER_RIGHT_6_PENALTY=0.124655
PRIMER_LEFT_6_SEQUENCE=AAGTGGGAGGAGGAGGAAGT
PRIMER_RIGHT_6_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_6=294,20
PRIMER_RIGHT_6=1233,20
PRIMER_LEFT_6_TM=59.151
PRIMER_RIGHT_6_TM=59.875
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=1.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.4000
PRIMER_PAIR_6_COMPL_ANY=2.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=940
PRIMER_PAIR_7_PENALTY=1.048263
PRIMER_LEFT_7_PENALTY=0.010545
PRIMER_RIGHT_7_PENALTY=1.037717
PRIMER_LEFT_7_SEQUENCE=GGAGCTTTTGGACGAGACAG
PRIMER_RIGHT_7_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_7=384,20
PRIMER_RIGHT_7=1241,21
PRIMER_LEFT_7_TM=59.989
PRIMER_RIGHT_7_TM=60.038
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=858
PRIMER_PAIR_8_PENALTY=1.223660
PRIMER_LEFT_8_PENALTY=0.185942
PRIMER_RIGHT_8_PENALTY=1.037717
PRIMER_LEFT_8_SEQUENCE=GAAGTGGGAGGAGGAGGAAG
PRIMER_RIGHT_8_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_8=293,20
PRIMER_RIGHT_8=1241,21
PRIMER_LEFT_8_TM=60.186
PRIMER_RIGHT_8_TM=60.038
PRIMER_LEFT_8_GC_PERCENT=60.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=1.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=949
PRIMER_PAIR_9_PENALTY=1.227368
PRIMER_LEFT_9_PENALTY=1.102713
PRIMER_RIGHT_9_PENALTY=0.124655
PRIMER_LEFT_9_SEQUENCE=CCGAGTGTATAAGCCGATGAA
PRIMER_RIGHT_9_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_9=508,21
PRIMER_RIGHT_9=1233,20
PRIMER_LEFT_9_TM=60.103
PRIMER_RIGHT_9_TM=59.875
PRIMER_LEFT_9_GC_PERCENT=47.619
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=7.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=726
PRIMER_PAIR_10_PENALTY=1.240620
PRIMER_LEFT_10_PENALTY=1.115965
PRIMER_RIGHT_10_PENALTY=0.124655
PRIMER_LEFT_10_SEQUENCE=AGCTTTTGGACGAGACAGCA
PRIMER_RIGHT_10_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_10=386,20
PRIMER_RIGHT_10=1233,20
PRIMER_LEFT_10_TM=61.116
PRIMER_RIGHT_10_TM=59.875
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.4000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=848
PRIMER_PAIR_11_PENALTY=1.257373
PRIMER_LEFT_11_PENALTY=1.132718
PRIMER_RIGHT_11_PENALTY=0.124655
PRIMER_LEFT_11_SEQUENCE=TTTTGGACGAGACAGCAGATT
PRIMER_RIGHT_11_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_11=389,21
PRIMER_RIGHT_11=1233,20
PRIMER_LEFT_11_TM=59.867
PRIMER_RIGHT_11_TM=59.875
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=7.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=845
PRIMER_PAIR_12_PENALTY=1.277574
PRIMER_LEFT_12_PENALTY=0.239857
PRIMER_RIGHT_12_PENALTY=1.037717
PRIMER_LEFT_12_SEQUENCE=TCATCACGAGTTGGACGAAA
PRIMER_RIGHT_12_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_12=621,20
PRIMER_RIGHT_12=1241,21
PRIMER_LEFT_12_TM=60.240
PRIMER_RIGHT_12_TM=60.038
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.0000
PRIMER_RIGHT_12_END_STABILITY=9.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=621
PRIMER_PAIR_13_PENALTY=1.322013
PRIMER_LEFT_13_PENALTY=0.239857
PRIMER_RIGHT_13_PENALTY=1.082156
PRIMER_LEFT_13_SEQUENCE=TCATCACGAGTTGGACGAAA
PRIMER_RIGHT_13_SEQUENCE=CCTGATCCATTTTTGTGAGGA
PRIMER_LEFT_13=621,20
PRIMER_RIGHT_13=1452,21
PRIMER_LEFT_13_TM=60.240
PRIMER_RIGHT_13_TM=59.918
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.0000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=832
PRIMER_PAIR_14_PENALTY=1.346439
PRIMER_LEFT_14_PENALTY=0.308722
PRIMER_RIGHT_14_PENALTY=1.037717
PRIMER_LEFT_14_SEQUENCE=CATCACGAGTTGGACGAAAA
PRIMER_RIGHT_14_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_14=622,20
PRIMER_RIGHT_14=1241,21
PRIMER_LEFT_14_TM=59.691
PRIMER_RIGHT_14_TM=60.038
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=620
PRIMER_PAIR_15_PENALTY=1.377402
PRIMER_LEFT_15_PENALTY=1.252746
PRIMER_RIGHT_15_PENALTY=0.124655
PRIMER_LEFT_15_SEQUENCE=CCGAGTGTATAAGCCGATGA
PRIMER_RIGHT_15_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_15=508,20
PRIMER_RIGHT_15=1233,20
PRIMER_LEFT_15_TM=58.747
PRIMER_RIGHT_15_TM=59.875
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=726
PRIMER_PAIR_16_PENALTY=1.390878
PRIMER_LEFT_16_PENALTY=0.308722
PRIMER_RIGHT_16_PENALTY=1.082156
PRIMER_LEFT_16_SEQUENCE=CATCACGAGTTGGACGAAAA
PRIMER_RIGHT_16_SEQUENCE=CCTGATCCATTTTTGTGAGGA
PRIMER_LEFT_16=622,20
PRIMER_RIGHT_16=1452,21
PRIMER_LEFT_16_TM=59.691
PRIMER_RIGHT_16_TM=59.918
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=831
PRIMER_PAIR_17_PENALTY=1.390878
PRIMER_LEFT_17_PENALTY=0.308722
PRIMER_RIGHT_17_PENALTY=1.082156
PRIMER_LEFT_17_SEQUENCE=CATCACGAGTTGGACGAAAA
PRIMER_RIGHT_17_SEQUENCE=TCCTGATCCATTTTTGTGAGG
PRIMER_LEFT_17=622,20
PRIMER_RIGHT_17=1453,21
PRIMER_LEFT_17_TM=59.691
PRIMER_RIGHT_17_TM=59.918
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=832
PRIMER_PAIR_18_PENALTY=1.398038
PRIMER_LEFT_18_PENALTY=0.360320
PRIMER_RIGHT_18_PENALTY=1.037717
PRIMER_LEFT_18_SEQUENCE=ACTAATCTCGGCTCGGCTTT
PRIMER_RIGHT_18_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_18=484,20
PRIMER_RIGHT_18=1241,21
PRIMER_LEFT_18_TM=60.360
PRIMER_RIGHT_18_TM=60.038
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=9.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PAIR_18_PRODUCT_SIZE=758
PRIMER_PAIR_19_PENALTY=1.403656
PRIMER_LEFT_19_PENALTY=1.279001
PRIMER_RIGHT_19_PENALTY=0.124655
PRIMER_LEFT_19_SEQUENCE=ATCAGGGGAAGTGGTGGAAG
PRIMER_RIGHT_19_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_19=277,20
PRIMER_RIGHT_19=1233,20
PRIMER_LEFT_19_TM=61.279
PRIMER_RIGHT_19_TM=59.875
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=957
PRIMER_PAIR_20_PENALTY=1.442476
PRIMER_LEFT_20_PENALTY=0.360320
PRIMER_RIGHT_20_PENALTY=1.082156
PRIMER_LEFT_20_SEQUENCE=ACTAATCTCGGCTCGGCTTT
PRIMER_RIGHT_20_SEQUENCE=CCTGATCCATTTTTGTGAGGA
PRIMER_LEFT_20=484,20
PRIMER_RIGHT_20=1452,21
PRIMER_LEFT_20_TM=60.360
PRIMER_RIGHT_20_TM=59.918
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=969
PRIMER_PAIR_21_PENALTY=1.442476
PRIMER_LEFT_21_PENALTY=0.360320
PRIMER_RIGHT_21_PENALTY=1.082156
PRIMER_LEFT_21_SEQUENCE=ACTAATCTCGGCTCGGCTTT
PRIMER_RIGHT_21_SEQUENCE=TCCTGATCCATTTTTGTGAGG
PRIMER_LEFT_21=484,20
PRIMER_RIGHT_21=1453,21
PRIMER_LEFT_21_TM=60.360
PRIMER_RIGHT_21_TM=59.918
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=970
PRIMER_PAIR_22_PENALTY=1.544369
PRIMER_LEFT_22_PENALTY=1.419714
PRIMER_RIGHT_22_PENALTY=0.124655
PRIMER_LEFT_22_SEQUENCE=GCCGTCTACTAATCTCGGCT
PRIMER_RIGHT_22_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_22=477,20
PRIMER_RIGHT_22=1233,20
PRIMER_LEFT_22_TM=58.580
PRIMER_RIGHT_22_TM=59.875
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=11.4000
PRIMER_RIGHT_22_END_STABILITY=7.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=757
PRIMER_PAIR_23_PENALTY=1.566919
PRIMER_LEFT_23_PENALTY=1.442263
PRIMER_RIGHT_23_PENALTY=0.124655
PRIMER_LEFT_23_SEQUENCE=TAGCCGAGTGTATAAGCCGA
PRIMER_RIGHT_23_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_23=505,20
PRIMER_RIGHT_23=1233,20
PRIMER_LEFT_23_TM=58.558
PRIMER_RIGHT_23_TM=59.875
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=11.4000
PRIMER_RIGHT_23_END_STABILITY=7.4000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=729
PRIMER_PAIR_24_PENALTY=1.582603
PRIMER_LEFT_24_PENALTY=0.239857
PRIMER_RIGHT_24_PENALTY=1.342747
PRIMER_LEFT_24_SEQUENCE=TCATCACGAGTTGGACGAAA
PRIMER_RIGHT_24_SEQUENCE=CCAAACTAAACTGGTTGGCTG
PRIMER_LEFT_24=621,20
PRIMER_RIGHT_24=1528,21
PRIMER_LEFT_24_TM=60.240
PRIMER_RIGHT_24_TM=59.657
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=9.0000
PRIMER_RIGHT_24_END_STABILITY=9.7000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=908
PRIMER_PAIR_25_PENALTY=1.616576
PRIMER_LEFT_25_PENALTY=1.491921
PRIMER_RIGHT_25_PENALTY=0.124655
PRIMER_LEFT_25_SEQUENCE=AGTGGTGGAAGTGGGAGGAG
PRIMER_RIGHT_25_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_25=286,20
PRIMER_RIGHT_25=1233,20
PRIMER_LEFT_25_TM=61.492
PRIMER_RIGHT_25_TM=59.875
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=1.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=7.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=948
PRIMER_PAIR_26_PENALTY=1.635190
PRIMER_LEFT_26_PENALTY=1.510535
PRIMER_RIGHT_26_PENALTY=0.124655
PRIMER_LEFT_26_SEQUENCE=ATCATCACGAGTTGGACGAAA
PRIMER_RIGHT_26_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_26=620,21
PRIMER_RIGHT_26=1233,20
PRIMER_LEFT_26_TM=60.511
PRIMER_RIGHT_26_TM=59.875
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=9.0000
PRIMER_RIGHT_26_END_STABILITY=7.4000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=614
PRIMER_PAIR_27_PENALTY=1.639857
PRIMER_LEFT_27_PENALTY=1.515201
PRIMER_RIGHT_27_PENALTY=0.124655
PRIMER_LEFT_27_SEQUENCE=TCAGCCGTCTACTAATCTCGG
PRIMER_RIGHT_27_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_27=474,21
PRIMER_RIGHT_27=1233,20
PRIMER_LEFT_27_TM=59.485
PRIMER_RIGHT_27_TM=59.875
PRIMER_LEFT_27_GC_PERCENT=52.381
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=5.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=7.4000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=760
PRIMER_PAIR_28_PENALTY=1.651468
PRIMER_LEFT_28_PENALTY=0.308722
PRIMER_RIGHT_28_PENALTY=1.342747
PRIMER_LEFT_28_SEQUENCE=CATCACGAGTTGGACGAAAA
PRIMER_RIGHT_28_SEQUENCE=CCAAACTAAACTGGTTGGCTG
PRIMER_LEFT_28=622,20
PRIMER_RIGHT_28=1528,21
PRIMER_LEFT_28_TM=59.691
PRIMER_RIGHT_28_TM=59.657
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.3000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=907
PRIMER_PAIR_29_PENALTY=1.664089
PRIMER_LEFT_29_PENALTY=0.626371
PRIMER_RIGHT_29_PENALTY=1.037717
PRIMER_LEFT_29_SEQUENCE=GCCGAGTGTATAAGCCGATG
PRIMER_RIGHT_29_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_29=507,20
PRIMER_RIGHT_29=1241,21
PRIMER_LEFT_29_TM=60.626
PRIMER_RIGHT_29_TM=60.038
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=8.6000
PRIMER_RIGHT_29_END_STABILITY=9.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=735
PRIMER_PAIR_30_PENALTY=1.669483
PRIMER_LEFT_30_PENALTY=1.544828
PRIMER_RIGHT_30_PENALTY=0.124655
PRIMER_LEFT_30_SEQUENCE=TGGACGAGACAGCAGATTACA
PRIMER_RIGHT_30_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_30=392,21
PRIMER_RIGHT_30=1233,20
PRIMER_LEFT_30_TM=59.455
PRIMER_RIGHT_30_TM=59.875
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.0000
PRIMER_RIGHT_30_END_STABILITY=7.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=842
PRIMER_PAIR_31_PENALTY=1.669715
PRIMER_LEFT_31_PENALTY=0.010545
PRIMER_RIGHT_31_PENALTY=1.659170
PRIMER_LEFT_31_SEQUENCE=GGAGCTTTTGGACGAGACAG
PRIMER_RIGHT_31_SEQUENCE=GTCATGCACATGCCTTCTCC
PRIMER_LEFT_31=384,20
PRIMER_RIGHT_31=1209,20
PRIMER_LEFT_31_TM=59.989
PRIMER_RIGHT_31_TM=61.659
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=826
PRIMER_PAIR_32_PENALTY=1.681869
PRIMER_LEFT_32_PENALTY=1.557214
PRIMER_RIGHT_32_PENALTY=0.124655
PRIMER_LEFT_32_SEQUENCE=TTTGGACGAGACAGCAGATT
PRIMER_RIGHT_32_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_32=390,20
PRIMER_RIGHT_32=1233,20
PRIMER_LEFT_32_TM=58.443
PRIMER_RIGHT_32_TM=59.875
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.6000
PRIMER_RIGHT_32_END_STABILITY=7.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=844
PRIMER_PAIR_33_PENALTY=1.708527
PRIMER_LEFT_33_PENALTY=0.626371
PRIMER_RIGHT_33_PENALTY=1.082156
PRIMER_LEFT_33_SEQUENCE=GCCGAGTGTATAAGCCGATG
PRIMER_RIGHT_33_SEQUENCE=CCTGATCCATTTTTGTGAGGA
PRIMER_LEFT_33=507,20
PRIMER_RIGHT_33=1452,21
PRIMER_LEFT_33_TM=60.626
PRIMER_RIGHT_33_TM=59.918
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.6000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=946
PRIMER_PAIR_34_PENALTY=1.708527
PRIMER_LEFT_34_PENALTY=0.626371
PRIMER_RIGHT_34_PENALTY=1.082156
PRIMER_LEFT_34_SEQUENCE=GCCGAGTGTATAAGCCGATG
PRIMER_RIGHT_34_SEQUENCE=TCCTGATCCATTTTTGTGAGG
PRIMER_LEFT_34=507,20
PRIMER_RIGHT_34=1453,21
PRIMER_LEFT_34_TM=60.626
PRIMER_RIGHT_34_TM=59.918
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.6000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=947
PRIMER_PAIR_35_PENALTY=1.807593
PRIMER_LEFT_35_PENALTY=0.010545
PRIMER_RIGHT_35_PENALTY=1.797048
PRIMER_LEFT_35_SEQUENCE=GGAGCTTTTGGACGAGACAG
PRIMER_RIGHT_35_SEQUENCE=AGCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_35=384,20
PRIMER_RIGHT_35=1234,21
PRIMER_LEFT_35_TM=59.989
PRIMER_RIGHT_35_TM=60.797
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=52.381
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.4000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=851
PRIMER_PAIR_36_PENALTY=1.856073
PRIMER_LEFT_36_PENALTY=0.196903
PRIMER_RIGHT_36_PENALTY=1.659170
PRIMER_LEFT_36_SEQUENCE=GATTCGGAACGAGTCTGAGG
PRIMER_RIGHT_36_SEQUENCE=GTCATGCACATGCCTTCTCC
PRIMER_LEFT_36=215,20
PRIMER_RIGHT_36=1209,20
PRIMER_LEFT_36_TM=59.803
PRIMER_RIGHT_36_TM=61.659
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=995
PRIMER_PAIR_37_PENALTY=1.876669
PRIMER_LEFT_37_PENALTY=0.838952
PRIMER_RIGHT_37_PENALTY=1.037717
PRIMER_LEFT_37_SEQUENCE=CTTTTGGACGAGACAGCAGA
PRIMER_RIGHT_37_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_37=388,20
PRIMER_RIGHT_37=1241,21
PRIMER_LEFT_37_TM=59.161
PRIMER_RIGHT_37_TM=60.038
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=6.00
PRIMER_PAIR_37_COMPL_END=2.00
PRIMER_PAIR_37_PRODUCT_SIZE=854
PRIMER_PAIR_38_PENALTY=1.887043
PRIMER_LEFT_38_PENALTY=0.849325
PRIMER_RIGHT_38_PENALTY=1.037717
PRIMER_LEFT_38_SEQUENCE=AAGTGGGAGGAGGAGGAAGT
PRIMER_RIGHT_38_SEQUENCE=CACTTTGAGCAAAAGAGTCGG
PRIMER_LEFT_38=294,20
PRIMER_RIGHT_38=1241,21
PRIMER_LEFT_38_TM=59.151
PRIMER_RIGHT_38_TM=60.038
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=1.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=9.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=948
PRIMER_PAIR_39_PENALTY=1.899027
PRIMER_LEFT_39_PENALTY=0.239857
PRIMER_RIGHT_39_PENALTY=1.659170
PRIMER_LEFT_39_SEQUENCE=TCATCACGAGTTGGACGAAA
PRIMER_RIGHT_39_SEQUENCE=GTCATGCACATGCCTTCTCC
PRIMER_LEFT_39=621,20
PRIMER_RIGHT_39=1209,20
PRIMER_LEFT_39_TM=60.240
PRIMER_RIGHT_39_TM=61.659
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=9.0000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=589
PRIMER_PAIR_40_PENALTY=1.967892
PRIMER_LEFT_40_PENALTY=0.308722
PRIMER_RIGHT_40_PENALTY=1.659170
PRIMER_LEFT_40_SEQUENCE=CATCACGAGTTGGACGAAAA
PRIMER_RIGHT_40_SEQUENCE=GTCATGCACATGCCTTCTCC
PRIMER_LEFT_40=622,20
PRIMER_RIGHT_40=1209,20
PRIMER_LEFT_40_TM=59.691
PRIMER_RIGHT_40_TM=61.659
PRIMER_LEFT_40_GC_PERCENT=45.000
PRIMER_RIGHT_40_GC_PERCENT=55.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.3000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=588
PRIMER_PAIR_41_PENALTY=1.982991
PRIMER_LEFT_41_PENALTY=0.185942
PRIMER_RIGHT_41_PENALTY=1.797048
PRIMER_LEFT_41_SEQUENCE=GAAGTGGGAGGAGGAGGAAG
PRIMER_RIGHT_41_SEQUENCE=AGCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_41=293,20
PRIMER_RIGHT_41=1234,21
PRIMER_LEFT_41_TM=60.186
PRIMER_RIGHT_41_TM=60.797
PRIMER_LEFT_41_GC_PERCENT=60.000
PRIMER_RIGHT_41_GC_PERCENT=52.381
PRIMER_LEFT_41_SELF_ANY=1.00
PRIMER_RIGHT_41_SELF_ANY=5.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.4000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=942
PRIMER_PAIR_42_PENALTY=1.996294
PRIMER_LEFT_42_PENALTY=1.871638
PRIMER_RIGHT_42_PENALTY=0.124655
PRIMER_LEFT_42_SEQUENCE=AAGTGGTGGAAGTGGGAGGA
PRIMER_RIGHT_42_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_42=285,20
PRIMER_RIGHT_42=1233,20
PRIMER_LEFT_42_TM=61.872
PRIMER_RIGHT_42_TM=59.875
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=1.00
PRIMER_RIGHT_42_SELF_ANY=5.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.9000
PRIMER_RIGHT_42_END_STABILITY=7.4000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=949
PRIMER_PAIR_43_PENALTY=1.996294
PRIMER_LEFT_43_PENALTY=1.871638
PRIMER_RIGHT_43_PENALTY=0.124655
PRIMER_LEFT_43_SEQUENCE=GAAGTGGTGGAAGTGGGAGG
PRIMER_RIGHT_43_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_43=284,20
PRIMER_RIGHT_43=1233,20
PRIMER_LEFT_43_TM=61.872
PRIMER_RIGHT_43_TM=59.875
PRIMER_LEFT_43_GC_PERCENT=60.000
PRIMER_RIGHT_43_GC_PERCENT=55.000
PRIMER_LEFT_43_SELF_ANY=1.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.4000
PRIMER_RIGHT_43_END_STABILITY=7.4000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=950
PRIMER_PAIR_44_PENALTY=1.996294
PRIMER_LEFT_44_PENALTY=1.871638
PRIMER_RIGHT_44_PENALTY=0.124655
PRIMER_LEFT_44_SEQUENCE=GGAAGTGGTGGAAGTGGGAG
PRIMER_RIGHT_44_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_44=283,20
PRIMER_RIGHT_44=1233,20
PRIMER_LEFT_44_TM=61.872
PRIMER_RIGHT_44_TM=59.875
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=55.000
PRIMER_LEFT_44_SELF_ANY=1.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.4000
PRIMER_RIGHT_44_END_STABILITY=7.4000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=951
PRIMER_PAIR_45_PENALTY=1.996294
PRIMER_LEFT_45_PENALTY=1.871638
PRIMER_RIGHT_45_PENALTY=0.124655
PRIMER_LEFT_45_SEQUENCE=TCAGGGGAAGTGGTGGAAGT
PRIMER_RIGHT_45_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_45=278,20
PRIMER_RIGHT_45=1233,20
PRIMER_LEFT_45_TM=61.872
PRIMER_RIGHT_45_TM=59.875
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=5.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=7.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=956
PRIMER_PAIR_46_PENALTY=2.019490
PRIMER_LEFT_46_PENALTY=0.360320
PRIMER_RIGHT_46_PENALTY=1.659170
PRIMER_LEFT_46_SEQUENCE=ACTAATCTCGGCTCGGCTTT
PRIMER_RIGHT_46_SEQUENCE=GTCATGCACATGCCTTCTCC
PRIMER_LEFT_46=484,20
PRIMER_RIGHT_46=1209,20
PRIMER_LEFT_46_TM=60.360
PRIMER_RIGHT_46_TM=61.659
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.5000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=726
PRIMER_PAIR_47_PENALTY=2.019627
PRIMER_LEFT_47_PENALTY=1.894971
PRIMER_RIGHT_47_PENALTY=0.124655
PRIMER_LEFT_47_SEQUENCE=CGTTACCGGAGCTTTTGGAC
PRIMER_RIGHT_47_SEQUENCE=GCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_47=377,20
PRIMER_RIGHT_47=1233,20
PRIMER_LEFT_47_TM=61.895
PRIMER_RIGHT_47_TM=59.875
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=5.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=7.4000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=857
PRIMER_PAIR_48_PENALTY=2.036905
PRIMER_LEFT_48_PENALTY=0.239857
PRIMER_RIGHT_48_PENALTY=1.797048
PRIMER_LEFT_48_SEQUENCE=TCATCACGAGTTGGACGAAA
PRIMER_RIGHT_48_SEQUENCE=AGCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_48=621,20
PRIMER_RIGHT_48=1234,21
PRIMER_LEFT_48_TM=60.240
PRIMER_RIGHT_48_TM=60.797
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=5.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.0000
PRIMER_RIGHT_48_END_STABILITY=7.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=614
PRIMER_PAIR_49_PENALTY=2.105770
PRIMER_LEFT_49_PENALTY=0.308722
PRIMER_RIGHT_49_PENALTY=1.797048
PRIMER_LEFT_49_SEQUENCE=CATCACGAGTTGGACGAAAA
PRIMER_RIGHT_49_SEQUENCE=AGCAAAAGAGTCGGACCGTAG
PRIMER_LEFT_49=622,20
PRIMER_RIGHT_49=1234,21
PRIMER_LEFT_49_TM=59.691
PRIMER_RIGHT_49_TM=60.797
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=52.381
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=7.4000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=613
=
SEQUENCE_ID=MAGIC.105_4_17444348_17444513_hi-0_bur-0_6491_29072_TRUE
SEQUENCE_TARGET=950,265
SEQUENCE_TEMPLATE=CAAGGAGTATTACCAAGACACTGNTCTTGATTCTCATCGACAATCTCTNCTTAAGCTANCTGAAGTTAANCCTTATGTGTCTCCTTCTCTCTTTGTCCGTTCTGCTTTCTCTCCTACTGCTTCGATGCTTAAGATTGATGCGGAGGAAGAGGATAAGTTGGAGGAGATNTTGAGAGATCATGTTANTCCAGCTGCTAAGGAGGTTCTNGAGGTTTGGTTGGAGCGGTGTGTGAAGGAAGAAGANGAGAAGATTGTGNTTGGGGAAGAAGAGAGAATGGAGTTGGAGAGAAGAGATAAAAGCTTTAGAAGGAAGAGNATAGAGGANGATTTGGATTTGCAGTTTCCGNGAATGTTTGGTGAAGAAGTTTCCTCCCGTGTTGTACACGCTATTAAAGAAGCTTTCGGTGTTCTCTAGATTATGTAACTNTATNTATNNNNNAGNTAATGTTCATTGTACTAAACATNTACTTTCATCAATGTCACAAGAACGCAAGANGNCNATCAAACAAGTCCTGTTTTCTCCTAAGAACGTCTTTGTTGTNAATCATCTCTGAATCGGATTCCTTCNGATTCATTTTAATACTATGGGATTGTAAATTTATCAATCAAGTTTCNNNNNTNTCTGNAAAAAAAANNNTTGAATTATTNTTTTTCTCATNGATTTNAAANTTTTTTTNTCACTATTTTNCATGGATGTTTTGTTAAATTTCTCGTTCTCNAGATCATGTCATTTTACATTAGCAAAACCAATATAATATATAGATTAATATTAANGAAGGAACATAAACGTGCAAAACTAAAAGTTTTAAAGCTAAATCGCTTTCAAAAGAGTTAGTAACCAGANAAAAAAGAGACAGATCATCNGCAACTAATCTCGTCATCGCATGCATCATAAACCAAATTAACNAAATCATNCATTTAATCCATTTGTTCTNATCAGATCATCCTTTTCCTTCCTACACAANAAATGGTTAGTNTTTTCTTTTCTCGATNGTCTCTTNGTTTAGATTCTTTATCNGACTACTTTGTTCATCGGTTTTGTTTTTCTTTTTGTCTTCCGGAAGGCGAATTACATGTCGGAAGCAGCACAGCTCAGAAGAGGTCTAAAGCCTAAAGGGAAGACTTATGGGTTGACCAATCAGAAGAGACGAGAGATCAGAGAGATNTTTGATCTTTTCGACATAGACGGTTCAGGTAAACTATTAAACCGATTNTTTATCCGATTTANNNNNNNNNNNCTCACATGATTTAGTGCATCATTTCATATGTAGGTAGCATCGATGCTAGCGAGCTCAACGTTGCTATGAGGTCAACGGCAATTTTTCTTTCTTTTTCAAAGTTATTTTCTTCAAGTTCTAGTTAAGTATAGTACTAAAAAATTATCAGCTGATTACAGGTCTCTTGGATTTGAGATGAATAATCAGGTTTGTTTGTTTTCCNTCCTTTTTTCTTCTTTGTTTTAATNTCATGGTTTAGATCATTGTACGTAGAACCAACTTGTATTTAGTTTGGTCATGTTCATTGTTGCAGCAAATAAACGAATTGATGGCAGAAGTAGATAAAAACCAAAGTGGAGCCATAGATTTCGACGAATTTGTGCATATGATGACAACCAAATTCGGAGAACGAGACTCCATAGACGAATTGTCTAAGGCGTTTAAGATCATTGACCACGACAATAATGTGAATAAATCTAAACCTGTCATTATTTCATTCTTTTTAATATTATCTTTTTTTGTTTGGATTGATTTCATTGTGTTTGACTGGACAACTTTTGGAATGTTTTAGGGGAAGATTTCACCTCGTGATATAAAGATGATTGCTAAAGAATTGGGAGAAAATTTCACAGATAATGATATAGAAGAAATGATCGAAGAAGCAGACCGTGACAGTAAGCAAAAACACATAATAATTTTGCGTTTTATCTATAATCGGTCCCTCAAGTTCATGTTTTTGCCTTAACCTACGCAGAAGATGGAGAAGTTAACTTGGAGGAGTTCATGAAGATGATGAAGAGAACCTCTTNCGGCTAAGTATAAATCAATTAGTAATGGTTGTGAATAATATTTGTTAATCACCTTGATTTATAATAATAAAGAAGTTTGATTTGTGGCTTGGTCGAATAAAAATGTATTGTTGTATAAAAATAATAATGTAATTCACAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.108309
PRIMER_LEFT_0_PENALTY=0.079056
PRIMER_RIGHT_0_PENALTY=0.029253
PRIMER_LEFT_0_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_0_SEQUENCE=CGTTCTCCGAATTTGGTTGT
PRIMER_LEFT_0=878,20
PRIMER_RIGHT_0=1627,20
PRIMER_LEFT_0_TM=59.921
PRIMER_RIGHT_0_TM=59.971
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.4000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=750
PRIMER_PAIR_1_PENALTY=1.011992
PRIMER_LEFT_1_PENALTY=0.079056
PRIMER_RIGHT_1_PENALTY=0.932936
PRIMER_LEFT_1_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_1_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_1=878,20
PRIMER_RIGHT_1=1326,20
PRIMER_LEFT_1_TM=59.921
PRIMER_RIGHT_1_TM=59.067
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=449
PRIMER_PAIR_2_PENALTY=1.126781
PRIMER_LEFT_2_PENALTY=0.079056
PRIMER_RIGHT_2_PENALTY=1.047725
PRIMER_LEFT_2_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_2_SEQUENCE=TGGTCAATGATCTTAAACGCC
PRIMER_LEFT_2=878,20
PRIMER_RIGHT_2=1672,21
PRIMER_LEFT_2_TM=59.921
PRIMER_RIGHT_2_TM=59.952
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=795
PRIMER_PAIR_3_PENALTY=1.383582
PRIMER_LEFT_3_PENALTY=0.079056
PRIMER_RIGHT_3_PENALTY=1.304526
PRIMER_LEFT_3_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_3_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_3=878,20
PRIMER_RIGHT_3=1325,20
PRIMER_LEFT_3_TM=59.921
PRIMER_RIGHT_3_TM=58.695
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=448
PRIMER_PAIR_4_PENALTY=1.407230
PRIMER_LEFT_4_PENALTY=0.474295
PRIMER_RIGHT_4_PENALTY=0.932936
PRIMER_LEFT_4_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_4_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_4=881,20
PRIMER_RIGHT_4=1326,20
PRIMER_LEFT_4_TM=60.474
PRIMER_RIGHT_4_TM=59.067
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=446
PRIMER_PAIR_5_PENALTY=1.522020
PRIMER_LEFT_5_PENALTY=0.474295
PRIMER_RIGHT_5_PENALTY=1.047725
PRIMER_LEFT_5_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_5_SEQUENCE=TGGTCAATGATCTTAAACGCC
PRIMER_LEFT_5=881,20
PRIMER_RIGHT_5=1672,21
PRIMER_LEFT_5_TM=60.474
PRIMER_RIGHT_5_TM=59.952
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.8000
PRIMER_RIGHT_5_END_STABILITY=11.1000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=792
PRIMER_PAIR_6_PENALTY=1.749872
PRIMER_LEFT_6_PENALTY=0.816936
PRIMER_RIGHT_6_PENALTY=0.932936
PRIMER_LEFT_6_SEQUENCE=AGAAGTTTCCTCCCGTGTTG
PRIMER_RIGHT_6_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_6=360,20
PRIMER_RIGHT_6=1326,20
PRIMER_LEFT_6_TM=59.183
PRIMER_RIGHT_6_TM=59.067
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=967
PRIMER_PAIR_7_PENALTY=1.778821
PRIMER_LEFT_7_PENALTY=0.474295
PRIMER_RIGHT_7_PENALTY=1.304526
PRIMER_LEFT_7_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_7_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_7=881,20
PRIMER_RIGHT_7=1325,20
PRIMER_LEFT_7_TM=60.474
PRIMER_RIGHT_7_TM=58.695
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.8000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=445
PRIMER_PAIR_8_PENALTY=1.804289
PRIMER_LEFT_8_PENALTY=0.079056
PRIMER_RIGHT_8_PENALTY=1.725232
PRIMER_LEFT_8_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_8_SEQUENCE=TGCTGCAACAATGAACATGAC
PRIMER_LEFT_8=878,20
PRIMER_RIGHT_8=1531,21
PRIMER_LEFT_8_TM=59.921
PRIMER_RIGHT_8_TM=60.725
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.3000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=654
PRIMER_PAIR_9_PENALTY=1.893080
PRIMER_LEFT_9_PENALTY=0.960145
PRIMER_RIGHT_9_PENALTY=0.932936
PRIMER_LEFT_9_SEQUENCE=GAAGTTTCCTCCCGTGTTGT
PRIMER_RIGHT_9_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_9=361,20
PRIMER_RIGHT_9=1326,20
PRIMER_LEFT_9_TM=59.040
PRIMER_RIGHT_9_TM=59.067
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=966
PRIMER_PAIR_10_PENALTY=1.942676
PRIMER_LEFT_10_PENALTY=1.913423
PRIMER_RIGHT_10_PENALTY=0.029253
PRIMER_LEFT_10_SEQUENCE=TCATCGCATGCATCATAAAC
PRIMER_RIGHT_10_SEQUENCE=CGTTCTCCGAATTTGGTTGT
PRIMER_LEFT_10=877,20
PRIMER_RIGHT_10=1627,20
PRIMER_LEFT_10_TM=58.087
PRIMER_RIGHT_10_TM=59.971
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.0000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=751
PRIMER_PAIR_11_PENALTY=1.949067
PRIMER_LEFT_11_PENALTY=1.016131
PRIMER_RIGHT_11_PENALTY=0.932936
PRIMER_LEFT_11_SEQUENCE=TCGCATGCATCATAAACCAA
PRIMER_RIGHT_11_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_11=880,20
PRIMER_RIGHT_11=1326,20
PRIMER_LEFT_11_TM=61.016
PRIMER_RIGHT_11_TM=59.067
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=447
PRIMER_PAIR_12_PENALTY=1.956675
PRIMER_LEFT_12_PENALTY=1.023740
PRIMER_RIGHT_12_PENALTY=0.932936
PRIMER_LEFT_12_SEQUENCE=AGAAGTTTCCTCCCGTGTTGT
PRIMER_RIGHT_12_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_12=360,21
PRIMER_RIGHT_12=1326,20
PRIMER_LEFT_12_TM=60.024
PRIMER_RIGHT_12_TM=59.067
PRIMER_LEFT_12_GC_PERCENT=47.619
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=967
PRIMER_PAIR_13_PENALTY=2.036748
PRIMER_LEFT_13_PENALTY=1.103813
PRIMER_RIGHT_13_PENALTY=0.932936
PRIMER_LEFT_13_SEQUENCE=CAATGTCACAAGAACGCAAGA
PRIMER_RIGHT_13_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_13=474,21
PRIMER_RIGHT_13=1326,20
PRIMER_LEFT_13_TM=59.896
PRIMER_RIGHT_13_TM=59.067
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=853
PRIMER_PAIR_14_PENALTY=2.036748
PRIMER_LEFT_14_PENALTY=1.103813
PRIMER_RIGHT_14_PENALTY=0.932936
PRIMER_LEFT_14_SEQUENCE=TCAATGTCACAAGAACGCAAG
PRIMER_RIGHT_14_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_14=473,21
PRIMER_RIGHT_14=1326,20
PRIMER_LEFT_14_TM=59.896
PRIMER_RIGHT_14_TM=59.067
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=854
PRIMER_PAIR_15_PENALTY=2.063857
PRIMER_LEFT_15_PENALTY=1.016131
PRIMER_RIGHT_15_PENALTY=1.047725
PRIMER_LEFT_15_SEQUENCE=TCGCATGCATCATAAACCAA
PRIMER_RIGHT_15_SEQUENCE=TGGTCAATGATCTTAAACGCC
PRIMER_LEFT_15=880,20
PRIMER_RIGHT_15=1672,21
PRIMER_LEFT_15_TM=61.016
PRIMER_RIGHT_15_TM=59.952
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=11.1000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=793
PRIMER_PAIR_16_PENALTY=2.097795
PRIMER_LEFT_16_PENALTY=1.164859
PRIMER_RIGHT_16_PENALTY=0.932936
PRIMER_LEFT_16_SEQUENCE=TCAATGTCACAAGAACGCAA
PRIMER_RIGHT_16_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_16=473,20
PRIMER_RIGHT_16=1326,20
PRIMER_LEFT_16_TM=58.835
PRIMER_RIGHT_16_TM=59.067
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=10.5000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=854
PRIMER_PAIR_17_PENALTY=2.121462
PRIMER_LEFT_17_PENALTY=0.816936
PRIMER_RIGHT_17_PENALTY=1.304526
PRIMER_LEFT_17_SEQUENCE=AGAAGTTTCCTCCCGTGTTG
PRIMER_RIGHT_17_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_17=360,20
PRIMER_RIGHT_17=1325,20
PRIMER_LEFT_17_TM=59.183
PRIMER_RIGHT_17_TM=58.695
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=966
PRIMER_PAIR_18_PENALTY=2.124168
PRIMER_LEFT_18_PENALTY=0.079056
PRIMER_RIGHT_18_PENALTY=2.045112
PRIMER_LEFT_18_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_18_SEQUENCE=CCAAAAGTTGTCCAGTCAAACA
PRIMER_LEFT_18=878,20
PRIMER_RIGHT_18=1777,22
PRIMER_LEFT_18_TM=59.921
PRIMER_RIGHT_18_TM=60.045
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.909
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=900
PRIMER_PAIR_19_PENALTY=2.174501
PRIMER_LEFT_19_PENALTY=0.079056
PRIMER_RIGHT_19_PENALTY=2.095445
PRIMER_LEFT_19_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_19_SEQUENCE=TCGAAATCTATGGCTCCACTTT
PRIMER_LEFT_19=878,20
PRIMER_RIGHT_19=1588,22
PRIMER_LEFT_19_TM=59.921
PRIMER_RIGHT_19_TM=60.095
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=40.909
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=711
PRIMER_PAIR_20_PENALTY=2.199527
PRIMER_LEFT_20_PENALTY=0.474295
PRIMER_RIGHT_20_PENALTY=1.725232
PRIMER_LEFT_20_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_20_SEQUENCE=TGCTGCAACAATGAACATGAC
PRIMER_LEFT_20=881,20
PRIMER_RIGHT_20=1531,21
PRIMER_LEFT_20_TM=60.474
PRIMER_RIGHT_20_TM=60.725
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.8000
PRIMER_RIGHT_20_END_STABILITY=6.3000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=651
PRIMER_PAIR_21_PENALTY=2.257884
PRIMER_LEFT_21_PENALTY=0.079056
PRIMER_RIGHT_21_PENALTY=2.178828
PRIMER_LEFT_21_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_21_SEQUENCE=CTTCTGCCATCAATTCGTTT
PRIMER_LEFT_21=878,20
PRIMER_RIGHT_21=1554,20
PRIMER_LEFT_21_TM=59.921
PRIMER_RIGHT_21_TM=57.821
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=40.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=677
PRIMER_PAIR_22_PENALTY=2.264670
PRIMER_LEFT_22_PENALTY=0.960145
PRIMER_RIGHT_22_PENALTY=1.304526
PRIMER_LEFT_22_SEQUENCE=GAAGTTTCCTCCCGTGTTGT
PRIMER_RIGHT_22_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_22=361,20
PRIMER_RIGHT_22=1325,20
PRIMER_LEFT_22_TM=59.040
PRIMER_RIGHT_22_TM=58.695
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=7.6000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=965
PRIMER_PAIR_23_PENALTY=2.278428
PRIMER_LEFT_23_PENALTY=0.079056
PRIMER_RIGHT_23_PENALTY=2.199371
PRIMER_LEFT_23_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_23_SEQUENCE=TCGAAATCTATGGCTCCACTT
PRIMER_LEFT_23=878,20
PRIMER_RIGHT_23=1588,21
PRIMER_LEFT_23_TM=59.921
PRIMER_RIGHT_23_TM=58.801
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=42.857
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=711
PRIMER_PAIR_24_PENALTY=2.320657
PRIMER_LEFT_24_PENALTY=1.016131
PRIMER_RIGHT_24_PENALTY=1.304526
PRIMER_LEFT_24_SEQUENCE=TCGCATGCATCATAAACCAA
PRIMER_RIGHT_24_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_24=880,20
PRIMER_RIGHT_24=1325,20
PRIMER_LEFT_24_TM=61.016
PRIMER_RIGHT_24_TM=58.695
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=446
PRIMER_PAIR_25_PENALTY=2.325704
PRIMER_LEFT_25_PENALTY=1.392768
PRIMER_RIGHT_25_PENALTY=0.932936
PRIMER_LEFT_25_SEQUENCE=GAAGAAGTTTCCTCCCGTGTT
PRIMER_RIGHT_25_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_25=358,21
PRIMER_RIGHT_25=1326,20
PRIMER_LEFT_25_TM=59.607
PRIMER_RIGHT_25_TM=59.067
PRIMER_LEFT_25_GC_PERCENT=47.619
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=969
PRIMER_PAIR_26_PENALTY=2.328266
PRIMER_LEFT_26_PENALTY=1.023740
PRIMER_RIGHT_26_PENALTY=1.304526
PRIMER_LEFT_26_SEQUENCE=AGAAGTTTCCTCCCGTGTTGT
PRIMER_RIGHT_26_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_26=360,21
PRIMER_RIGHT_26=1325,20
PRIMER_LEFT_26_TM=60.024
PRIMER_RIGHT_26_TM=58.695
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=966
PRIMER_PAIR_27_PENALTY=2.357386
PRIMER_LEFT_27_PENALTY=0.079056
PRIMER_RIGHT_27_PENALTY=2.278329
PRIMER_LEFT_27_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_27_SEQUENCE=GCAACAATGAACATGACCAAAC
PRIMER_LEFT_27=878,20
PRIMER_RIGHT_27=1527,22
PRIMER_LEFT_27_TM=59.921
PRIMER_RIGHT_27_TM=60.278
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=40.909
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=650
PRIMER_PAIR_28_PENALTY=2.408339
PRIMER_LEFT_28_PENALTY=1.103813
PRIMER_RIGHT_28_PENALTY=1.304526
PRIMER_LEFT_28_SEQUENCE=CAATGTCACAAGAACGCAAGA
PRIMER_RIGHT_28_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_28=474,21
PRIMER_RIGHT_28=1325,20
PRIMER_LEFT_28_TM=59.896
PRIMER_RIGHT_28_TM=58.695
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.0000
PRIMER_RIGHT_28_END_STABILITY=7.6000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=852
PRIMER_PAIR_29_PENALTY=2.408339
PRIMER_LEFT_29_PENALTY=1.103813
PRIMER_RIGHT_29_PENALTY=1.304526
PRIMER_LEFT_29_SEQUENCE=TCAATGTCACAAGAACGCAAG
PRIMER_RIGHT_29_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_29=473,21
PRIMER_RIGHT_29=1325,20
PRIMER_LEFT_29_TM=59.896
PRIMER_RIGHT_29_TM=58.695
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.5000
PRIMER_RIGHT_29_END_STABILITY=7.6000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=853
PRIMER_PAIR_30_PENALTY=2.410793
PRIMER_LEFT_30_PENALTY=0.079056
PRIMER_RIGHT_30_PENALTY=2.331737
PRIMER_LEFT_30_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_30_SEQUENCE=GCTGCAACAATGAACATGAC
PRIMER_LEFT_30=878,20
PRIMER_RIGHT_30=1530,20
PRIMER_LEFT_30_TM=59.921
PRIMER_RIGHT_30_TM=57.668
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=6.3000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=653
PRIMER_PAIR_31_PENALTY=2.446692
PRIMER_LEFT_31_PENALTY=1.513757
PRIMER_RIGHT_31_PENALTY=0.932936
PRIMER_LEFT_31_SEQUENCE=AAGAAGTTTCCTCCCGTGTTG
PRIMER_RIGHT_31_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_31=359,21
PRIMER_RIGHT_31=1326,20
PRIMER_LEFT_31_TM=60.514
PRIMER_RIGHT_31_TM=59.067
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=7.9000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=968
PRIMER_PAIR_32_PENALTY=2.458995
PRIMER_LEFT_32_PENALTY=0.079056
PRIMER_RIGHT_32_PENALTY=2.379939
PRIMER_LEFT_32_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_32_SEQUENCE=GTCGAAATCTATGGCTCCACTT
PRIMER_LEFT_32=878,20
PRIMER_RIGHT_32=1589,22
PRIMER_LEFT_32_TM=59.921
PRIMER_RIGHT_32_TM=59.620
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=712
PRIMER_PAIR_33_PENALTY=2.469385
PRIMER_LEFT_33_PENALTY=1.164859
PRIMER_RIGHT_33_PENALTY=1.304526
PRIMER_LEFT_33_SEQUENCE=TCAATGTCACAAGAACGCAA
PRIMER_RIGHT_33_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_33=473,20
PRIMER_RIGHT_33=1325,20
PRIMER_LEFT_33_TM=58.835
PRIMER_RIGHT_33_TM=58.695
PRIMER_LEFT_33_GC_PERCENT=40.000
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=10.5000
PRIMER_RIGHT_33_END_STABILITY=7.6000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=853
PRIMER_PAIR_34_PENALTY=2.519407
PRIMER_LEFT_34_PENALTY=0.474295
PRIMER_RIGHT_34_PENALTY=2.045112
PRIMER_LEFT_34_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_34_SEQUENCE=CCAAAAGTTGTCCAGTCAAACA
PRIMER_LEFT_34=881,20
PRIMER_RIGHT_34=1777,22
PRIMER_LEFT_34_TM=60.474
PRIMER_RIGHT_34_TM=60.045
PRIMER_LEFT_34_GC_PERCENT=40.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.8000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=897
PRIMER_PAIR_35_PENALTY=2.569740
PRIMER_LEFT_35_PENALTY=0.474295
PRIMER_RIGHT_35_PENALTY=2.095445
PRIMER_LEFT_35_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_35_SEQUENCE=TCGAAATCTATGGCTCCACTTT
PRIMER_LEFT_35=881,20
PRIMER_RIGHT_35=1588,22
PRIMER_LEFT_35_TM=60.474
PRIMER_RIGHT_35_TM=60.095
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=40.909
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.8000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=708
PRIMER_PAIR_36_PENALTY=2.586977
PRIMER_LEFT_36_PENALTY=2.557724
PRIMER_RIGHT_36_PENALTY=0.029253
PRIMER_LEFT_36_SEQUENCE=CAACTAATCTCGTCATCGCA
PRIMER_RIGHT_36_SEQUENCE=CGTTCTCCGAATTTGGTTGT
PRIMER_LEFT_36=865,20
PRIMER_RIGHT_36=1627,20
PRIMER_LEFT_36_TM=57.442
PRIMER_RIGHT_36_TM=59.971
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.2000
PRIMER_RIGHT_36_END_STABILITY=6.4000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=763
PRIMER_PAIR_37_PENALTY=2.647720
PRIMER_LEFT_37_PENALTY=1.714785
PRIMER_RIGHT_37_PENALTY=0.932936
PRIMER_LEFT_37_SEQUENCE=CATCAATGTCACAAGAACGCA
PRIMER_RIGHT_37_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_37=471,21
PRIMER_RIGHT_37=1326,20
PRIMER_LEFT_37_TM=60.715
PRIMER_RIGHT_37_TM=59.067
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.9000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=856
PRIMER_PAIR_38_PENALTY=2.673666
PRIMER_LEFT_38_PENALTY=0.474295
PRIMER_RIGHT_38_PENALTY=2.199371
PRIMER_LEFT_38_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_38_SEQUENCE=TCGAAATCTATGGCTCCACTT
PRIMER_LEFT_38=881,20
PRIMER_RIGHT_38=1588,21
PRIMER_LEFT_38_TM=60.474
PRIMER_RIGHT_38_TM=58.801
PRIMER_LEFT_38_GC_PERCENT=40.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.8000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=708
PRIMER_PAIR_39_PENALTY=2.697294
PRIMER_LEFT_39_PENALTY=1.392768
PRIMER_RIGHT_39_PENALTY=1.304526
PRIMER_LEFT_39_SEQUENCE=GAAGAAGTTTCCTCCCGTGTT
PRIMER_RIGHT_39_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_39=358,21
PRIMER_RIGHT_39=1325,20
PRIMER_LEFT_39_TM=59.607
PRIMER_RIGHT_39_TM=58.695
PRIMER_LEFT_39_GC_PERCENT=47.619
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.6000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=968
PRIMER_PAIR_40_PENALTY=2.704938
PRIMER_LEFT_40_PENALTY=1.772002
PRIMER_RIGHT_40_PENALTY=0.932936
PRIMER_LEFT_40_SEQUENCE=GAAGAAGTTTCCTCCCGTGT
PRIMER_RIGHT_40_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_40=358,20
PRIMER_RIGHT_40=1326,20
PRIMER_LEFT_40_TM=58.228
PRIMER_RIGHT_40_TM=59.067
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.1000
PRIMER_RIGHT_40_END_STABILITY=7.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=969
PRIMER_PAIR_41_PENALTY=2.704938
PRIMER_LEFT_41_PENALTY=1.772002
PRIMER_RIGHT_41_PENALTY=0.932936
PRIMER_LEFT_41_SEQUENCE=GTGAAGAAGTTTCCTCCCGT
PRIMER_RIGHT_41_SEQUENCE=AAGAAAAATTGCCGTTGACC
PRIMER_LEFT_41=356,20
PRIMER_RIGHT_41=1326,20
PRIMER_LEFT_41_TM=58.228
PRIMER_RIGHT_41_TM=59.067
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=11.1000
PRIMER_RIGHT_41_END_STABILITY=7.9000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=971
PRIMER_PAIR_42_PENALTY=2.728603
PRIMER_LEFT_42_PENALTY=0.079056
PRIMER_RIGHT_42_PENALTY=2.649547
PRIMER_LEFT_42_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_42_SEQUENCE=GGTGAAATCTTCCCCTAAAACA
PRIMER_LEFT_42=878,20
PRIMER_RIGHT_42=1801,22
PRIMER_LEFT_42_TM=59.921
PRIMER_RIGHT_42_TM=59.350
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.2000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=924
PRIMER_PAIR_43_PENALTY=2.738484
PRIMER_LEFT_43_PENALTY=0.079056
PRIMER_RIGHT_43_PENALTY=2.659428
PRIMER_LEFT_43_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_43_SEQUENCE=CGAAATCTATGGCTCCACTTT
PRIMER_LEFT_43=878,20
PRIMER_RIGHT_43=1587,21
PRIMER_LEFT_43_TM=59.921
PRIMER_RIGHT_43_TM=58.341
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.2000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=710
PRIMER_PAIR_44_PENALTY=2.741364
PRIMER_LEFT_44_PENALTY=1.016131
PRIMER_RIGHT_44_PENALTY=1.725232
PRIMER_LEFT_44_SEQUENCE=TCGCATGCATCATAAACCAA
PRIMER_RIGHT_44_SEQUENCE=TGCTGCAACAATGAACATGAC
PRIMER_LEFT_44=880,20
PRIMER_RIGHT_44=1531,21
PRIMER_LEFT_44_TM=61.016
PRIMER_RIGHT_44_TM=60.725
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=6.3000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=652
PRIMER_PAIR_45_PENALTY=2.752624
PRIMER_LEFT_45_PENALTY=0.474295
PRIMER_RIGHT_45_PENALTY=2.278329
PRIMER_LEFT_45_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_45_SEQUENCE=GCAACAATGAACATGACCAAAC
PRIMER_LEFT_45=881,20
PRIMER_RIGHT_45=1527,22
PRIMER_LEFT_45_TM=60.474
PRIMER_RIGHT_45_TM=60.278
PRIMER_LEFT_45_GC_PERCENT=40.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=6.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.8000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=647
PRIMER_PAIR_46_PENALTY=2.805906
PRIMER_LEFT_46_PENALTY=2.776653
PRIMER_RIGHT_46_PENALTY=0.029253
PRIMER_LEFT_46_SEQUENCE=TCATCGCATGCATCATAAACC
PRIMER_RIGHT_46_SEQUENCE=CGTTCTCCGAATTTGGTTGT
PRIMER_LEFT_46=877,21
PRIMER_RIGHT_46=1627,20
PRIMER_LEFT_46_TM=61.777
PRIMER_RIGHT_46_TM=59.971
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=6.4000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=751
PRIMER_PAIR_47_PENALTY=2.806032
PRIMER_LEFT_47_PENALTY=0.474295
PRIMER_RIGHT_47_PENALTY=2.331737
PRIMER_LEFT_47_SEQUENCE=CGCATGCATCATAAACCAAA
PRIMER_RIGHT_47_SEQUENCE=GCTGCAACAATGAACATGAC
PRIMER_LEFT_47=881,20
PRIMER_RIGHT_47=1530,20
PRIMER_LEFT_47_TM=60.474
PRIMER_RIGHT_47_TM=57.668
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=6.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.8000
PRIMER_RIGHT_47_END_STABILITY=6.3000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=650
PRIMER_PAIR_48_PENALTY=2.818282
PRIMER_LEFT_48_PENALTY=1.513757
PRIMER_RIGHT_48_PENALTY=1.304526
PRIMER_LEFT_48_SEQUENCE=AAGAAGTTTCCTCCCGTGTTG
PRIMER_RIGHT_48_SEQUENCE=AGAAAAATTGCCGTTGACCT
PRIMER_LEFT_48=359,21
PRIMER_RIGHT_48=1325,20
PRIMER_LEFT_48_TM=60.514
PRIMER_RIGHT_48_TM=58.695
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=40.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=7.6000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=967
PRIMER_PAIR_49_PENALTY=2.844106
PRIMER_LEFT_49_PENALTY=0.079056
PRIMER_RIGHT_49_PENALTY=2.765049
PRIMER_LEFT_49_SEQUENCE=CATCGCATGCATCATAAACC
PRIMER_RIGHT_49_SEQUENCE=CGTTCTCCGAATTTGGTTGTC
PRIMER_LEFT_49=878,20
PRIMER_RIGHT_49=1627,21
PRIMER_LEFT_49_TM=59.921
PRIMER_RIGHT_49_TM=61.765
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=47.619
PRIMER_LEFT_49_SELF_ANY=6.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=750
=
SEQUENCE_ID=MAGIC.105_4_13520037_13520350_po-0_sf-2_261761_70238_TRUE
SEQUENCE_TARGET=950,413
SEQUENCE_TEMPLATE=GAGCTTGCNAAAGCGGTTGAAGAATTAGGTGCNGGTGAAATACTATTGAACTGCATAGACTGTGATGGTACACTCAAAACTTCAATCTTTGAATTAGTTTCATGTCTGAAAATTTGGACCACCTTTACACACGATGAGAACTANNTATGATCCACGAAAAACAAAAAAGCACACAACCACCTTTTAAACTACACTGCTGCTGGAGTTAAAGATCAATGTATTATAAGTTTTTATCTCCTTATGATGCCTTCCTTCTCTGCGAGTTTCAGGTCAAGGGAAAGGATTCGACATAGACTTAGTAAAGCTCATCTCAGATTCAGTAGGCATACCGGTGATCGCAAGCAGTGGAGCAGGTACTCCCGNCCACTTTTCCGAGGTGTTTGAGAAGACAAACGCATCTGCCGCGCTTGCTGCCGGCATTTTNCACCGGAAAGAGGTAACCNTTTTTNCTCTTGTTAAAACCTTTTGCNTTTATACCTGGTAACACTATTATTCTACTATTTTGTATAAAGGTACCAATCCAATCTGTGAAAGAGCACTTACAAGAGGAGCGCATAGAAGTCAGGATCTGAGAATTTTCTGGTCTGCGTGTTACCATAATTCATGNCACATTCAACAAGATAGAATCTTTACCTTCAATAAATTAAATTAAGTCGGAACCATCGGAGCTCCAATAGGTCTGAGTGAATCCTCTTATAGTTGATTTCCTNTTGTTGACAGTAAGACTGATACAATTTATGGATTTCCCTTGAAAGTAAGAAAGCAANGTATGTTCACTTTCTCAGCTTTTCTATTTAATACAAAGAAAGCATNTAACTTCTCTCTCGTCTAGATCTTATTAGGAANTCATTCAATCCGAAAAACCTCAAAAGGGTATCTTATTCAGTCAAAATTGAACATATCTGTATCAAACTGTGTAANAAGTAAGAACNTAGACACTGAGGCCTATTCTTCCAATCCCGCAATACTCGGGAAACTGTAAAGAAACTTGAAAGATAAGNATTGTTGTTACCCCCAAACGTTTTAAAGACACTGCAGGTCTATAACTGGNAAAAGCCAGACACTTTGTGACTCCTCGAAACCAAATNTTTTATATGTCAAGAAGAAGCCTTTGTGGATCCTCCACAACATCCTTGACACGGCGCAGGAAGTACACAGCCTCTCTACCATCAATCAGCCTGTGATCATANGTAAGTGCGACATACATCATTGGTCTNGGCACTACGCTTCCTCCTACTACCATTGGACGTGACACAATCGAGTGCATNCCGAGTATNGCAGACTGCAAAACCACANGTTNCTGTTAANAAAAANCGTGGTTCTGANTGNGACCCGGTGANATGGGAAATNAATTTAAACCTGAGGAGGNTTAATGATCGGAGTGCTTATGAGACTTCCATAGACACCGCCATTTGAAACGGTGAATGATCCNCCAGCCATCTCATCTATGGATATGGTTCCTTCATTTGCCTTCTTGGCAAGTGAGTTTATCGTCTTCTCTATCTCGGCAAAGTTCATTTTATCAGCACCTCTTATGACTGGTACTACAAGTCCCTGGATAAGAGACCACAAGAANAAAANTTCAGCCTCACATACATGATCGCAATAAGNATGAAATTTAGCACAAACCAATGATANCTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCTAACAGCAGCCTGAGACAGATAACGAAGAATANGGTATTANNTTTGCTATCATTTAACTCTGGCATTGTATTATNAATATCGANAAATAAGTAANTNNATACTTTAATNAATCCAGACATAAGCCCCAACTTNACTCCATGCTTTTCATAAAATGCATCCTTGTATTGNGATCGNAGCTTCATCAAATTTGTCCTACACNCAGAAAANAGCTCCATAATNAGCAAANAAAATCAAACTGACTAGTNTAATTCAGTGCACCAAAAGGACTCGCATAANTGATATAGACATATCAACTTGACGGGTTTAAGCTGTTACTCACATATCAACTTCATTGAAAGTTGTCAGCAANGCNAANGTATTTTGAGAGTCTTTCAATCTTGTTGCAACTCGTTTCCGAAGTCTTGTCATCGGAACCTGCAGTGTTGTTAGATGTCATATCANTGATNATGTGACTGTAAAGCTTTTTATTAGGTAACAANCTAACTTTNNNNNNNNNNNNNNNNNNNATTTNAAAATTTNCAGACGGAAGCCAGGNAAAATGATAGATTTTGCAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.111155
PRIMER_LEFT_0_PENALTY=0.066323
PRIMER_RIGHT_0_PENALTY=0.044832
PRIMER_LEFT_0_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_0_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_0=648,20
PRIMER_RIGHT_0=1412,20
PRIMER_LEFT_0_TM=60.066
PRIMER_RIGHT_0_TM=59.955
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=9.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=765
PRIMER_PAIR_1_PENALTY=0.117668
PRIMER_LEFT_1_PENALTY=0.066323
PRIMER_RIGHT_1_PENALTY=0.051345
PRIMER_LEFT_1_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_1_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_1=648,20
PRIMER_RIGHT_1=1473,20
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=826
PRIMER_PAIR_2_PENALTY=0.250267
PRIMER_LEFT_2_PENALTY=0.066323
PRIMER_RIGHT_2_PENALTY=0.183944
PRIMER_LEFT_2_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_2_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_2=648,20
PRIMER_RIGHT_2=1477,20
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=60.184
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=830
PRIMER_PAIR_3_PENALTY=0.447294
PRIMER_LEFT_3_PENALTY=0.402462
PRIMER_RIGHT_3_PENALTY=0.044832
PRIMER_LEFT_3_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_3_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_3=546,20
PRIMER_RIGHT_3=1412,20
PRIMER_LEFT_3_TM=59.598
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=867
PRIMER_PAIR_4_PENALTY=0.453807
PRIMER_LEFT_4_PENALTY=0.402462
PRIMER_RIGHT_4_PENALTY=0.051345
PRIMER_LEFT_4_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_4_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_4=546,20
PRIMER_RIGHT_4=1473,20
PRIMER_LEFT_4_TM=59.598
PRIMER_RIGHT_4_TM=60.051
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=928
PRIMER_PAIR_5_PENALTY=0.524045
PRIMER_LEFT_5_PENALTY=0.066323
PRIMER_RIGHT_5_PENALTY=0.457721
PRIMER_LEFT_5_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_5_SEQUENCE=TTCAAATGGCGGTGTCTATG
PRIMER_LEFT_5=648,20
PRIMER_RIGHT_5=1416,20
PRIMER_LEFT_5_TM=60.066
PRIMER_RIGHT_5_TM=59.542
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.9000
PRIMER_RIGHT_5_END_STABILITY=5.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=769
PRIMER_PAIR_6_PENALTY=0.586406
PRIMER_LEFT_6_PENALTY=0.402462
PRIMER_RIGHT_6_PENALTY=0.183944
PRIMER_LEFT_6_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_6_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_6=546,20
PRIMER_RIGHT_6=1477,20
PRIMER_LEFT_6_TM=59.598
PRIMER_RIGHT_6_TM=60.184
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=932
PRIMER_PAIR_7_PENALTY=0.631929
PRIMER_LEFT_7_PENALTY=0.066323
PRIMER_RIGHT_7_PENALTY=0.565606
PRIMER_LEFT_7_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_7_SEQUENCE=ATGGCGGTGTCTATGGAAGT
PRIMER_LEFT_7=648,20
PRIMER_RIGHT_7=1411,20
PRIMER_LEFT_7_TM=60.066
PRIMER_RIGHT_7_TM=59.434
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.9000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=764
PRIMER_PAIR_8_PENALTY=0.860184
PRIMER_LEFT_8_PENALTY=0.402462
PRIMER_RIGHT_8_PENALTY=0.457721
PRIMER_LEFT_8_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_8_SEQUENCE=TTCAAATGGCGGTGTCTATG
PRIMER_LEFT_8=546,20
PRIMER_RIGHT_8=1416,20
PRIMER_LEFT_8_TM=59.598
PRIMER_RIGHT_8_TM=59.542
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=5.9000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=871
PRIMER_PAIR_9_PENALTY=0.968068
PRIMER_LEFT_9_PENALTY=0.402462
PRIMER_RIGHT_9_PENALTY=0.565606
PRIMER_LEFT_9_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_9_SEQUENCE=ATGGCGGTGTCTATGGAAGT
PRIMER_LEFT_9=546,20
PRIMER_RIGHT_9=1411,20
PRIMER_LEFT_9_TM=59.598
PRIMER_RIGHT_9_TM=59.434
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=866
PRIMER_PAIR_10_PENALTY=1.169428
PRIMER_LEFT_10_PENALTY=0.066323
PRIMER_RIGHT_10_PENALTY=1.103105
PRIMER_LEFT_10_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_10_SEQUENCE=TTGCCGAGATAGAGAAGACGA
PRIMER_LEFT_10=648,20
PRIMER_RIGHT_10=1510,21
PRIMER_LEFT_10_TM=60.066
PRIMER_RIGHT_10_TM=60.103
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.9000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=863
PRIMER_PAIR_11_PENALTY=1.177549
PRIMER_LEFT_11_PENALTY=1.132718
PRIMER_RIGHT_11_PENALTY=0.044832
PRIMER_LEFT_11_SEQUENCE=GAGAATTTTCTGGTCTGCGTG
PRIMER_RIGHT_11_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_11=570,21
PRIMER_RIGHT_11=1412,20
PRIMER_LEFT_11_TM=59.867
PRIMER_RIGHT_11_TM=59.955
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=9.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=843
PRIMER_PAIR_12_PENALTY=1.177549
PRIMER_LEFT_12_PENALTY=1.132718
PRIMER_RIGHT_12_PENALTY=0.044832
PRIMER_LEFT_12_SEQUENCE=TGAGAATTTTCTGGTCTGCGT
PRIMER_RIGHT_12_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_12=569,21
PRIMER_RIGHT_12=1412,20
PRIMER_LEFT_12_TM=59.867
PRIMER_RIGHT_12_TM=59.955
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=844
PRIMER_PAIR_13_PENALTY=1.184063
PRIMER_LEFT_13_PENALTY=1.132718
PRIMER_RIGHT_13_PENALTY=0.051345
PRIMER_LEFT_13_SEQUENCE=GAGAATTTTCTGGTCTGCGTG
PRIMER_RIGHT_13_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_13=570,21
PRIMER_RIGHT_13=1473,20
PRIMER_LEFT_13_TM=59.867
PRIMER_RIGHT_13_TM=60.051
PRIMER_LEFT_13_GC_PERCENT=47.619
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=904
PRIMER_PAIR_14_PENALTY=1.184063
PRIMER_LEFT_14_PENALTY=1.132718
PRIMER_RIGHT_14_PENALTY=0.051345
PRIMER_LEFT_14_SEQUENCE=TGAGAATTTTCTGGTCTGCGT
PRIMER_RIGHT_14_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_14=569,21
PRIMER_RIGHT_14=1473,20
PRIMER_LEFT_14_TM=59.867
PRIMER_RIGHT_14_TM=60.051
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=905
PRIMER_PAIR_15_PENALTY=1.316662
PRIMER_LEFT_15_PENALTY=1.132718
PRIMER_RIGHT_15_PENALTY=0.183944
PRIMER_LEFT_15_SEQUENCE=GAGAATTTTCTGGTCTGCGTG
PRIMER_RIGHT_15_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_15=570,21
PRIMER_RIGHT_15=1477,20
PRIMER_LEFT_15_TM=59.867
PRIMER_RIGHT_15_TM=60.184
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.9000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=908
PRIMER_PAIR_16_PENALTY=1.316662
PRIMER_LEFT_16_PENALTY=1.132718
PRIMER_RIGHT_16_PENALTY=0.183944
PRIMER_LEFT_16_SEQUENCE=TGAGAATTTTCTGGTCTGCGT
PRIMER_RIGHT_16_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_16=569,21
PRIMER_RIGHT_16=1477,20
PRIMER_LEFT_16_TM=59.867
PRIMER_RIGHT_16_TM=60.184
PRIMER_LEFT_16_GC_PERCENT=42.857
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=909
PRIMER_PAIR_17_PENALTY=1.339231
PRIMER_LEFT_17_PENALTY=0.066323
PRIMER_RIGHT_17_PENALTY=1.272908
PRIMER_LEFT_17_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_17_SEQUENCE=TGCCAAGAAGGCAAATGAAG
PRIMER_LEFT_17=648,20
PRIMER_RIGHT_17=1479,20
PRIMER_LEFT_17_TM=60.066
PRIMER_RIGHT_17_TM=61.273
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=832
PRIMER_PAIR_18_PENALTY=1.342747
PRIMER_LEFT_18_PENALTY=1.297915
PRIMER_RIGHT_18_PENALTY=0.044832
PRIMER_LEFT_18_SEQUENCE=TCCGAAAAACCTCAAAAGGG
PRIMER_RIGHT_18_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_18=854,20
PRIMER_RIGHT_18=1412,20
PRIMER_LEFT_18_TM=61.298
PRIMER_RIGHT_18_TM=59.955
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=559
PRIMER_PAIR_19_PENALTY=1.349260
PRIMER_LEFT_19_PENALTY=1.297915
PRIMER_RIGHT_19_PENALTY=0.051345
PRIMER_LEFT_19_SEQUENCE=TCCGAAAAACCTCAAAAGGG
PRIMER_RIGHT_19_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_19=854,20
PRIMER_RIGHT_19=1473,20
PRIMER_LEFT_19_TM=61.298
PRIMER_RIGHT_19_TM=60.051
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=620
PRIMER_PAIR_20_PENALTY=1.369274
PRIMER_LEFT_20_PENALTY=0.066323
PRIMER_RIGHT_20_PENALTY=1.302950
PRIMER_LEFT_20_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_20_SEQUENCE=TGCCGAGATAGAGAAGACGA
PRIMER_LEFT_20=648,20
PRIMER_RIGHT_20=1509,20
PRIMER_LEFT_20_TM=60.066
PRIMER_RIGHT_20_TM=58.697
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.9000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=862
PRIMER_PAIR_21_PENALTY=1.370718
PRIMER_LEFT_21_PENALTY=1.325886
PRIMER_RIGHT_21_PENALTY=0.044832
PRIMER_LEFT_21_SEQUENCE=ATTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_21_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_21=647,21
PRIMER_RIGHT_21=1412,20
PRIMER_LEFT_21_TM=60.326
PRIMER_RIGHT_21_TM=59.955
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=766
PRIMER_PAIR_22_PENALTY=1.377231
PRIMER_LEFT_22_PENALTY=1.325886
PRIMER_RIGHT_22_PENALTY=0.051345
PRIMER_LEFT_22_SEQUENCE=ATTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_22_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_22=647,21
PRIMER_RIGHT_22=1473,20
PRIMER_LEFT_22_TM=60.326
PRIMER_RIGHT_22_TM=60.051
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=45.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.9000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=827
PRIMER_PAIR_23_PENALTY=1.449212
PRIMER_LEFT_23_PENALTY=0.066323
PRIMER_RIGHT_23_PENALTY=1.382888
PRIMER_LEFT_23_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_23_SEQUENCE=TCCATAGATGAGATGGCTGG
PRIMER_LEFT_23=648,20
PRIMER_RIGHT_23=1451,20
PRIMER_LEFT_23_TM=60.066
PRIMER_RIGHT_23_TM=58.617
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.9000
PRIMER_RIGHT_23_END_STABILITY=9.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=804
PRIMER_PAIR_24_PENALTY=1.481859
PRIMER_LEFT_24_PENALTY=1.297915
PRIMER_RIGHT_24_PENALTY=0.183944
PRIMER_LEFT_24_SEQUENCE=TCCGAAAAACCTCAAAAGGG
PRIMER_RIGHT_24_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_24=854,20
PRIMER_RIGHT_24=1477,20
PRIMER_LEFT_24_TM=61.298
PRIMER_RIGHT_24_TM=60.184
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=624
PRIMER_PAIR_25_PENALTY=1.505567
PRIMER_LEFT_25_PENALTY=0.402462
PRIMER_RIGHT_25_PENALTY=1.103105
PRIMER_LEFT_25_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_25_SEQUENCE=TTGCCGAGATAGAGAAGACGA
PRIMER_LEFT_25=546,20
PRIMER_RIGHT_25=1510,21
PRIMER_LEFT_25_TM=59.598
PRIMER_RIGHT_25_TM=60.103
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=8.1000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=965
PRIMER_PAIR_26_PENALTY=1.509830
PRIMER_LEFT_26_PENALTY=1.325886
PRIMER_RIGHT_26_PENALTY=0.183944
PRIMER_LEFT_26_SEQUENCE=ATTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_26_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_26=647,21
PRIMER_RIGHT_26=1477,20
PRIMER_LEFT_26_TM=60.326
PRIMER_RIGHT_26_TM=60.184
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=9.9000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=831
PRIMER_PAIR_27_PENALTY=1.590439
PRIMER_LEFT_27_PENALTY=1.132718
PRIMER_RIGHT_27_PENALTY=0.457721
PRIMER_LEFT_27_SEQUENCE=GAGAATTTTCTGGTCTGCGTG
PRIMER_RIGHT_27_SEQUENCE=TTCAAATGGCGGTGTCTATG
PRIMER_LEFT_27=570,21
PRIMER_RIGHT_27=1416,20
PRIMER_LEFT_27_TM=59.867
PRIMER_RIGHT_27_TM=59.542
PRIMER_LEFT_27_GC_PERCENT=47.619
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.9000
PRIMER_RIGHT_27_END_STABILITY=5.9000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=847
PRIMER_PAIR_28_PENALTY=1.590439
PRIMER_LEFT_28_PENALTY=1.132718
PRIMER_RIGHT_28_PENALTY=0.457721
PRIMER_LEFT_28_SEQUENCE=TGAGAATTTTCTGGTCTGCGT
PRIMER_RIGHT_28_SEQUENCE=TTCAAATGGCGGTGTCTATG
PRIMER_LEFT_28=569,21
PRIMER_RIGHT_28=1416,20
PRIMER_LEFT_28_TM=59.867
PRIMER_RIGHT_28_TM=59.542
PRIMER_LEFT_28_GC_PERCENT=42.857
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.9000
PRIMER_RIGHT_28_END_STABILITY=5.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=848
PRIMER_PAIR_29_PENALTY=1.675370
PRIMER_LEFT_29_PENALTY=0.402462
PRIMER_RIGHT_29_PENALTY=1.272908
PRIMER_LEFT_29_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_29_SEQUENCE=TGCCAAGAAGGCAAATGAAG
PRIMER_LEFT_29=546,20
PRIMER_RIGHT_29=1479,20
PRIMER_LEFT_29_TM=59.598
PRIMER_RIGHT_29_TM=61.273
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=934
PRIMER_PAIR_30_PENALTY=1.698324
PRIMER_LEFT_30_PENALTY=1.132718
PRIMER_RIGHT_30_PENALTY=0.565606
PRIMER_LEFT_30_SEQUENCE=GAGAATTTTCTGGTCTGCGTG
PRIMER_RIGHT_30_SEQUENCE=ATGGCGGTGTCTATGGAAGT
PRIMER_LEFT_30=570,21
PRIMER_RIGHT_30=1411,20
PRIMER_LEFT_30_TM=59.867
PRIMER_RIGHT_30_TM=59.434
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=9.9000
PRIMER_RIGHT_30_END_STABILITY=6.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=842
PRIMER_PAIR_31_PENALTY=1.698324
PRIMER_LEFT_31_PENALTY=1.132718
PRIMER_RIGHT_31_PENALTY=0.565606
PRIMER_LEFT_31_SEQUENCE=TGAGAATTTTCTGGTCTGCGT
PRIMER_RIGHT_31_SEQUENCE=ATGGCGGTGTCTATGGAAGT
PRIMER_LEFT_31=569,21
PRIMER_RIGHT_31=1411,20
PRIMER_LEFT_31_TM=59.867
PRIMER_RIGHT_31_TM=59.434
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.9000
PRIMER_RIGHT_31_END_STABILITY=6.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=843
PRIMER_PAIR_32_PENALTY=1.703168
PRIMER_LEFT_32_PENALTY=0.066323
PRIMER_RIGHT_32_PENALTY=1.636845
PRIMER_LEFT_32_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_32_SEQUENCE=TTGCCAAGAAGGCAAATGAA
PRIMER_LEFT_32=648,20
PRIMER_RIGHT_32=1480,20
PRIMER_LEFT_32_TM=60.066
PRIMER_RIGHT_32_TM=61.637
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=9.9000
PRIMER_RIGHT_32_END_STABILITY=6.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=833
PRIMER_PAIR_33_PENALTY=1.705412
PRIMER_LEFT_33_PENALTY=0.402462
PRIMER_RIGHT_33_PENALTY=1.302950
PRIMER_LEFT_33_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_33_SEQUENCE=TGCCGAGATAGAGAAGACGA
PRIMER_LEFT_33=546,20
PRIMER_RIGHT_33=1509,20
PRIMER_LEFT_33_TM=59.598
PRIMER_RIGHT_33_TM=58.697
PRIMER_LEFT_33_GC_PERCENT=55.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=8.1000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=964
PRIMER_PAIR_34_PENALTY=1.755637
PRIMER_LEFT_34_PENALTY=1.297915
PRIMER_RIGHT_34_PENALTY=0.457721
PRIMER_LEFT_34_SEQUENCE=TCCGAAAAACCTCAAAAGGG
PRIMER_RIGHT_34_SEQUENCE=TTCAAATGGCGGTGTCTATG
PRIMER_LEFT_34=854,20
PRIMER_RIGHT_34=1416,20
PRIMER_LEFT_34_TM=61.298
PRIMER_RIGHT_34_TM=59.542
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=5.9000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=563
PRIMER_PAIR_35_PENALTY=1.783608
PRIMER_LEFT_35_PENALTY=1.325886
PRIMER_RIGHT_35_PENALTY=0.457721
PRIMER_LEFT_35_SEQUENCE=ATTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_35_SEQUENCE=TTCAAATGGCGGTGTCTATG
PRIMER_LEFT_35=647,21
PRIMER_RIGHT_35=1416,20
PRIMER_LEFT_35_TM=60.326
PRIMER_RIGHT_35_TM=59.542
PRIMER_LEFT_35_GC_PERCENT=47.619
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.9000
PRIMER_RIGHT_35_END_STABILITY=5.9000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=770
PRIMER_PAIR_36_PENALTY=1.785350
PRIMER_LEFT_36_PENALTY=0.402462
PRIMER_RIGHT_36_PENALTY=1.382888
PRIMER_LEFT_36_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_36_SEQUENCE=TCCATAGATGAGATGGCTGG
PRIMER_LEFT_36=546,20
PRIMER_RIGHT_36=1451,20
PRIMER_LEFT_36_TM=59.598
PRIMER_RIGHT_36_TM=58.617
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=906
PRIMER_PAIR_37_PENALTY=1.812420
PRIMER_LEFT_37_PENALTY=1.767588
PRIMER_RIGHT_37_PENALTY=0.044832
PRIMER_LEFT_37_SEQUENCE=GAATTTTCTGGTCTGCGTGTT
PRIMER_RIGHT_37_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_37=572,21
PRIMER_RIGHT_37=1412,20
PRIMER_LEFT_37_TM=59.232
PRIMER_RIGHT_37_TM=59.955
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=841
PRIMER_PAIR_38_PENALTY=1.812420
PRIMER_LEFT_38_PENALTY=1.767588
PRIMER_RIGHT_38_PENALTY=0.044832
PRIMER_LEFT_38_SEQUENCE=AAGAGGAGCGCATAGAAGTCA
PRIMER_RIGHT_38_SEQUENCE=AATGGCGGTGTCTATGGAAG
PRIMER_LEFT_38=543,21
PRIMER_RIGHT_38=1412,20
PRIMER_LEFT_38_TM=59.232
PRIMER_RIGHT_38_TM=59.955
PRIMER_LEFT_38_GC_PERCENT=47.619
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=870
PRIMER_PAIR_39_PENALTY=1.818933
PRIMER_LEFT_39_PENALTY=1.767588
PRIMER_RIGHT_39_PENALTY=0.051345
PRIMER_LEFT_39_SEQUENCE=GAATTTTCTGGTCTGCGTGTT
PRIMER_RIGHT_39_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_39=572,21
PRIMER_RIGHT_39=1473,20
PRIMER_LEFT_39_TM=59.232
PRIMER_RIGHT_39_TM=60.051
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=902
PRIMER_PAIR_40_PENALTY=1.818933
PRIMER_LEFT_40_PENALTY=1.767588
PRIMER_RIGHT_40_PENALTY=0.051345
PRIMER_LEFT_40_SEQUENCE=AAGAGGAGCGCATAGAAGTCA
PRIMER_RIGHT_40_SEQUENCE=GAAGGCAAATGAAGGAACCA
PRIMER_LEFT_40=543,21
PRIMER_RIGHT_40=1473,20
PRIMER_LEFT_40_TM=59.232
PRIMER_RIGHT_40_TM=60.051
PRIMER_LEFT_40_GC_PERCENT=47.619
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=931
PRIMER_PAIR_41_PENALTY=1.823972
PRIMER_LEFT_41_PENALTY=0.066323
PRIMER_RIGHT_41_PENALTY=1.757649
PRIMER_LEFT_41_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_41_SEQUENCE=AATGGCGGTGTCTATGGAAGT
PRIMER_LEFT_41=648,20
PRIMER_RIGHT_41=1412,21
PRIMER_LEFT_41_TM=60.066
PRIMER_RIGHT_41_TM=60.758
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=9.9000
PRIMER_RIGHT_41_END_STABILITY=6.4000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=765
PRIMER_PAIR_42_PENALTY=1.863521
PRIMER_LEFT_42_PENALTY=1.297915
PRIMER_RIGHT_42_PENALTY=0.565606
PRIMER_LEFT_42_SEQUENCE=TCCGAAAAACCTCAAAAGGG
PRIMER_RIGHT_42_SEQUENCE=ATGGCGGTGTCTATGGAAGT
PRIMER_LEFT_42=854,20
PRIMER_RIGHT_42=1411,20
PRIMER_LEFT_42_TM=61.298
PRIMER_RIGHT_42_TM=59.434
PRIMER_LEFT_42_GC_PERCENT=45.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=9.7000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=558
PRIMER_PAIR_43_PENALTY=1.891492
PRIMER_LEFT_43_PENALTY=1.325886
PRIMER_RIGHT_43_PENALTY=0.565606
PRIMER_LEFT_43_SEQUENCE=ATTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_43_SEQUENCE=ATGGCGGTGTCTATGGAAGT
PRIMER_LEFT_43=647,21
PRIMER_RIGHT_43=1411,20
PRIMER_LEFT_43_TM=60.326
PRIMER_RIGHT_43_TM=59.434
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=6.4000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=765
PRIMER_PAIR_44_PENALTY=1.937285
PRIMER_LEFT_44_PENALTY=0.066323
PRIMER_RIGHT_44_PENALTY=1.870961
PRIMER_LEFT_44_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_44_SEQUENCE=TTTCAAATGGCGGTGTCTATG
PRIMER_LEFT_44=648,20
PRIMER_RIGHT_44=1417,21
PRIMER_LEFT_44_TM=60.066
PRIMER_RIGHT_44_TM=60.871
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=5.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=9.9000
PRIMER_RIGHT_44_END_STABILITY=5.9000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=770
PRIMER_PAIR_45_PENALTY=1.951532
PRIMER_LEFT_45_PENALTY=1.767588
PRIMER_RIGHT_45_PENALTY=0.183944
PRIMER_LEFT_45_SEQUENCE=GAATTTTCTGGTCTGCGTGTT
PRIMER_RIGHT_45_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_45=572,21
PRIMER_RIGHT_45=1477,20
PRIMER_LEFT_45_TM=59.232
PRIMER_RIGHT_45_TM=60.184
PRIMER_LEFT_45_GC_PERCENT=42.857
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=906
PRIMER_PAIR_46_PENALTY=1.951532
PRIMER_LEFT_46_PENALTY=1.767588
PRIMER_RIGHT_46_PENALTY=0.183944
PRIMER_LEFT_46_SEQUENCE=AAGAGGAGCGCATAGAAGTCA
PRIMER_RIGHT_46_SEQUENCE=CCAAGAAGGCAAATGAAGGA
PRIMER_LEFT_46=543,21
PRIMER_RIGHT_46=1477,20
PRIMER_LEFT_46_TM=59.232
PRIMER_RIGHT_46_TM=60.184
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=935
PRIMER_PAIR_47_PENALTY=1.997067
PRIMER_LEFT_47_PENALTY=0.066323
PRIMER_RIGHT_47_PENALTY=1.930744
PRIMER_LEFT_47_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_47_SEQUENCE=TTGCGATCATGTATGTGAGG
PRIMER_LEFT_47=648,20
PRIMER_RIGHT_47=1605,20
PRIMER_LEFT_47_TM=60.066
PRIMER_RIGHT_47_TM=58.069
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=958
PRIMER_PAIR_48_PENALTY=2.038621
PRIMER_LEFT_48_PENALTY=0.066323
PRIMER_RIGHT_48_PENALTY=1.972297
PRIMER_LEFT_48_SEQUENCE=TTAAGTCGGAACCATCGGAG
PRIMER_RIGHT_48_SEQUENCE=AGAAGGCAAATGAAGGAACCA
PRIMER_LEFT_48=648,20
PRIMER_RIGHT_48=1474,21
PRIMER_LEFT_48_TM=60.066
PRIMER_RIGHT_48_TM=60.972
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.9000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=827
PRIMER_PAIR_49_PENALTY=2.039307
PRIMER_LEFT_49_PENALTY=0.402462
PRIMER_RIGHT_49_PENALTY=1.636845
PRIMER_LEFT_49_SEQUENCE=AGGAGCGCATAGAAGTCAGG
PRIMER_RIGHT_49_SEQUENCE=TTGCCAAGAAGGCAAATGAA
PRIMER_LEFT_49=546,20
PRIMER_RIGHT_49=1480,20
PRIMER_LEFT_49_TM=59.598
PRIMER_RIGHT_49_TM=61.637
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=5.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=6.9000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=935
=
SEQUENCE_ID=MAGIC.338_3_14527790_14527840_ler-0_no-0_15916_86014_TRUE
SEQUENCE_TARGET=950,150
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_14037960_14038124_tsu-0_ler-0_77782_7509_TRUE
SEQUENCE_TARGET=950,264
SEQUENCE_TEMPLATE=TNAAAAATNNAGCNTCNNNNNNNNNCANCNNATTTATCCAAATTTAATTTCTAGTTTTGGAAGTGTTTGGGANAGCCCCNAATTAAAGTAGATACAACCACTAGGAATCCGTAATCTTCTCGGGGTCAACGATAGCCNGATCGGCTTCGGTTGGNTCAACAACAGCTGACTCAGTTTTGGTTGGTTCACNGGCAGAGAGTAGCTTAGAAGAGTTTCCCGGCTCAGTTCCGGCTTCATGTTCAACATCCGGGATGATCACTGTCTCACTNTTCACGANCGGACAGTAGTATCGACCANTTCACTAGAGCCTGAAGNGAACGTTTCAAGTCAGCAANAAACACAAATACAACAACTNNNGNNNATAANGNTNCAGATNTAAAAGNTTANCANCCCAATCTAAGCCTGATTCACTCAAANNCANNGNNNNGNNNTAANANTCCTCGAAGTGTTGAGGGTTAAAGGACAAGGTGGGAAGAGNAGCAAGGTGGCCTCTTTTCTCAGCTAAAACAGCCTCCAGTTTGGACCTTTCTTGTTCCTCAGAGAAATCTTCCTCAAGGGTTTGGTCCAACANAAGCAGGNTNCTTTCGTGCTNNTNNNNNNNNNNNTNNAGCTCCGATNTGGCNNTCTCNGTTTGAATGANNAGGATAGCNCCTTGNATCANNTCCATNCCGCGACNTTTCACNTCTCTTNNNNNNNNNNNNNNNNNNNTGGCTAATTCAGCATCNNANNNNNNNNNNNNNNNNTTGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNAGTCGAGAGTTCNTCCTNNAGCTTGTCNTANNTTTGNTACTGATCNTGCAACTCGCCATTNGANNTCATGAGCTTNNNNNNNAGATGNTCGNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAGAGGAGGAGCNNNNNNNNNNNNNNNGNGAAGANAANTTGCCTTTCCAAGCATCNATNTCCNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNGATCATGTNNTTAAGCTCNNTGTTCGCCTNAGCAANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACCCGANATGAATGATGNGCNTGGAGTAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGATNGTGTNTTTCCNNGTTTTGAAGCNNNGNAANACGTTGCCCTCTTTCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCGAGNGAAATNAANCCNTNGTTNGAACCTNTAAGCNATTTNGTNTGAGGAACGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTNGATCAGNCTTCTCNCCACTAGGTNGNNGAGAAGGNNNNNNNNNNNNNNNNGAGGAGGAGNACCCATCGATCNAGATGGAGGAGATGATCGNNGAGAGCGATCAGTNCGAGCCNTTGACTTTTCCCTCCAAGGAGAAGAGCTGTTNCGAGCTGGTCTCTCNCTCNNTNTNNNNATNAAGTCAGCGATTGGCTTGCTGCTCAGTCCATCACGGGCTNAAGTCCCGTGTGGTNTGGGAGGANGNGGTGGAAGATGATGGAGTTGTCCAGGAGGACTCATNTNGAGNNNNNNNNNNNNNNNNNNNNNNNNGTCGAGGGTAAACAAATTCCAAAGGGTTTCACCTTCGATGACCACTTTGAAGAANNNNNNNNATTCTTCAGTCGGAAGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAACAGAAGANANATATGNTTNTGCCCTAATTTCTACCAGGTTTNNTGGNCCANTTTGATGGAAGCACTTCGNAAAGGCTNNCAATNGAAGCAANGGNNNNNTTNATTAAGAACTANGGATGGTGNNANNNNNNNTNTTTGTTCGGNAGATNGGANATGAGGTTNNTGTTNGCATTNNNGTANGCNNAGAANTAACCNANTTTCTTCGANNAACTTCTTACNCTNAGAAGCTCNTTCAATTNGGNAACTNCNATTATGTACCCAGCNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14560690_14560882_po-0_ler-0_21820_2453_TRUE
SEQUENCE_TARGET=950,292
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCTGACCTTGGTAATTGATTGATTTGGAGCAAACCCTAATTATTCNNTAGAGGGATTTTACNAGGTATCTAAACTATCGATGGCAAAGACCTCTNANTTNTTCTTTGATAGTTTCTCATGCTTCATGTGATGNTGANTGTGTCCTTCNTCGAGTATAGATAGCAGTGAAATGTTGATGCTTTTCCNAACTTCTGAGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTGTTGGCATGCAACGTTTCTTCTCCATTGTTTCTATTGCTCCAATTTTNTGNATTTCATNTTCTCTATTANTTTTTAGTGTGGCCTCTNTTTATCAAGCCTNNCTCACATGNAAGGTTTNTCTTGGAAGAANGTAGGNTTTACTTCTCTTTCCAAAANTCTNAAATTTGAGANACAAAAGATTGANGATNTGTATACATTNAATTTCAGGTAAGTATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANTTTATTTTNTTTNCNTTAAACTTCCATGTATNAATTTTTTCNGTATCNCTATTANAGGATACANTTTTGAGAATNTCNNATGAACANTTACAACAGAGCAGCACAAAAAGAAATTACAANTAAAAACAGAAAGTAAGAGATAGGGTACATCATCAATTCAATTCAGGGANAAAAAAAGGCATTTGTAGGTGAGAACTGGACGAATCTNAACCATTTNGAGAGGTANNTCTCACCAAGTAAANGTGAAGACGCTGAANGTGTCCTTTACCTTAACATCATAAATTAATTATCCCGTATTTAGTACGGGTGAGAATACTAGTACCCTATAAGACGAAACCAACAAAGGAAATTTTGGGACTAATTCTAGTTAGAGTCTTGTAGAAGACGAATATACCACCACGGGATATTGTGNTTACTAAGAGTTTCATTNTTCTGNANGGATAGTTTTATATAGTAGCCGCCCATCGGTGGCATTCCNAGTAGTTCATATAGTATATATGCTTTAACATTTNGATTNTCTNAATGCAGAATTTGATATTATTTGCATACACTNTTACTAAGNTGCATATANTTTCANGAGCAGACAATTTACATTCCAAGTGTGGATTAATGATACTTAGTCGAGAAGNACACATTATTATTTATTTAGAGAAAGACAAGTAGAAGGGATNNNATACAATCTA
PRIMER_LEFT_NUM_RETURNED=18
PRIMER_RIGHT_NUM_RETURNED=18
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=18
PRIMER_PAIR_0_PENALTY=9.827710
PRIMER_LEFT_0_PENALTY=8.082680
PRIMER_RIGHT_0_PENALTY=1.745029
PRIMER_LEFT_0_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_0_SEQUENCE=TCTTTTTGTGCTGCTCTGTTG
PRIMER_LEFT_0=657,24
PRIMER_RIGHT_0=1619,21
PRIMER_LEFT_0_TM=64.083
PRIMER_RIGHT_0_TM=59.255
PRIMER_LEFT_0_GC_PERCENT=41.667
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=963
PRIMER_PAIR_1_PENALTY=10.178365
PRIMER_LEFT_1_PENALTY=8.082680
PRIMER_RIGHT_1_PENALTY=2.095684
PRIMER_LEFT_1_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_1_SEQUENCE=TCTTTTTGTGCTGCTCTGTTGT
PRIMER_LEFT_1=657,24
PRIMER_RIGHT_1=1619,22
PRIMER_LEFT_1_TM=64.083
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=963
PRIMER_PAIR_2_PENALTY=10.668939
PRIMER_LEFT_2_PENALTY=8.082680
PRIMER_RIGHT_2_PENALTY=2.586259
PRIMER_LEFT_2_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_2_SEQUENCE=TTCTTTTTGTGCTGCTCTGTTG
PRIMER_LEFT_2=657,24
PRIMER_RIGHT_2=1620,22
PRIMER_LEFT_2_TM=64.083
PRIMER_RIGHT_2_TM=60.586
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=964
PRIMER_PAIR_3_PENALTY=10.800449
PRIMER_LEFT_3_PENALTY=8.082680
PRIMER_RIGHT_3_PENALTY=2.717768
PRIMER_LEFT_3_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_3_SEQUENCE=CTTTTTGTGCTGCTCTGTTG
PRIMER_LEFT_3=657,24
PRIMER_RIGHT_3=1618,20
PRIMER_LEFT_3_TM=64.083
PRIMER_RIGHT_3_TM=57.282
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=962
PRIMER_PAIR_4_PENALTY=10.854816
PRIMER_LEFT_4_PENALTY=8.082680
PRIMER_RIGHT_4_PENALTY=2.772136
PRIMER_LEFT_4_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_4_SEQUENCE=CTTTTTGTGCTGCTCTGTTGT
PRIMER_LEFT_4=657,24
PRIMER_RIGHT_4=1618,21
PRIMER_LEFT_4_TM=64.083
PRIMER_RIGHT_4_TM=58.228
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=962
PRIMER_PAIR_5_PENALTY=10.964985
PRIMER_LEFT_5_PENALTY=8.082680
PRIMER_RIGHT_5_PENALTY=2.882305
PRIMER_LEFT_5_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_5_SEQUENCE=TTTTTGTGCTGCTCTGTTGT
PRIMER_LEFT_5=657,24
PRIMER_RIGHT_5=1617,20
PRIMER_LEFT_5_TM=64.083
PRIMER_RIGHT_5_TM=57.118
PRIMER_LEFT_5_GC_PERCENT=41.667
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=961
PRIMER_PAIR_6_PENALTY=11.201466
PRIMER_LEFT_6_PENALTY=8.082680
PRIMER_RIGHT_6_PENALTY=3.118785
PRIMER_LEFT_6_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_6_SEQUENCE=TTTCTTTTTGTGCTGCTCTG
PRIMER_LEFT_6=657,24
PRIMER_RIGHT_6=1621,20
PRIMER_LEFT_6_TM=64.083
PRIMER_RIGHT_6_TM=56.881
PRIMER_LEFT_6_GC_PERCENT=41.667
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=965
PRIMER_PAIR_7_PENALTY=11.473577
PRIMER_LEFT_7_PENALTY=9.728547
PRIMER_RIGHT_7_PENALTY=1.745029
PRIMER_LEFT_7_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_7_SEQUENCE=TCTTTTTGTGCTGCTCTGTTG
PRIMER_LEFT_7=656,25
PRIMER_RIGHT_7=1619,21
PRIMER_LEFT_7_TM=64.729
PRIMER_RIGHT_7_TM=59.255
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=964
PRIMER_PAIR_8_PENALTY=11.787153
PRIMER_LEFT_8_PENALTY=8.082680
PRIMER_RIGHT_8_PENALTY=3.704473
PRIMER_LEFT_8_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_8_SEQUENCE=TCTTTTTGTGCTGCTCTGTT
PRIMER_LEFT_8=657,24
PRIMER_RIGHT_8=1619,20
PRIMER_LEFT_8_TM=64.083
PRIMER_RIGHT_8_TM=56.296
PRIMER_LEFT_8_GC_PERCENT=41.667
PRIMER_RIGHT_8_GC_PERCENT=40.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=963
PRIMER_PAIR_9_PENALTY=11.787153
PRIMER_LEFT_9_PENALTY=8.082680
PRIMER_RIGHT_9_PENALTY=3.704473
PRIMER_LEFT_9_SEQUENCE=GCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_9_SEQUENCE=TTCTTTTTGTGCTGCTCTGT
PRIMER_LEFT_9=657,24
PRIMER_RIGHT_9=1620,20
PRIMER_LEFT_9_TM=64.083
PRIMER_RIGHT_9_TM=56.296
PRIMER_LEFT_9_GC_PERCENT=41.667
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=964
PRIMER_PAIR_10_PENALTY=11.824232
PRIMER_LEFT_10_PENALTY=9.728547
PRIMER_RIGHT_10_PENALTY=2.095684
PRIMER_LEFT_10_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_10_SEQUENCE=TCTTTTTGTGCTGCTCTGTTGT
PRIMER_LEFT_10=656,25
PRIMER_RIGHT_10=1619,22
PRIMER_LEFT_10_TM=64.729
PRIMER_RIGHT_10_TM=60.096
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=964
PRIMER_PAIR_11_PENALTY=12.314806
PRIMER_LEFT_11_PENALTY=9.728547
PRIMER_RIGHT_11_PENALTY=2.586259
PRIMER_LEFT_11_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_11_SEQUENCE=TTCTTTTTGTGCTGCTCTGTTG
PRIMER_LEFT_11=656,25
PRIMER_RIGHT_11=1620,22
PRIMER_LEFT_11_TM=64.729
PRIMER_RIGHT_11_TM=60.586
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.909
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=965
PRIMER_PAIR_12_PENALTY=12.446315
PRIMER_LEFT_12_PENALTY=9.728547
PRIMER_RIGHT_12_PENALTY=2.717768
PRIMER_LEFT_12_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_12_SEQUENCE=CTTTTTGTGCTGCTCTGTTG
PRIMER_LEFT_12=656,25
PRIMER_RIGHT_12=1618,20
PRIMER_LEFT_12_TM=64.729
PRIMER_RIGHT_12_TM=57.282
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=963
PRIMER_PAIR_13_PENALTY=12.500683
PRIMER_LEFT_13_PENALTY=9.728547
PRIMER_RIGHT_13_PENALTY=2.772136
PRIMER_LEFT_13_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_13_SEQUENCE=CTTTTTGTGCTGCTCTGTTGT
PRIMER_LEFT_13=656,25
PRIMER_RIGHT_13=1618,21
PRIMER_LEFT_13_TM=64.729
PRIMER_RIGHT_13_TM=58.228
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=963
PRIMER_PAIR_14_PENALTY=12.610852
PRIMER_LEFT_14_PENALTY=9.728547
PRIMER_RIGHT_14_PENALTY=2.882305
PRIMER_LEFT_14_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_14_SEQUENCE=TTTTTGTGCTGCTCTGTTGT
PRIMER_LEFT_14=656,25
PRIMER_RIGHT_14=1617,20
PRIMER_LEFT_14_TM=64.729
PRIMER_RIGHT_14_TM=57.118
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=962
PRIMER_PAIR_15_PENALTY=12.847333
PRIMER_LEFT_15_PENALTY=9.728547
PRIMER_RIGHT_15_PENALTY=3.118785
PRIMER_LEFT_15_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_15_SEQUENCE=TTTCTTTTTGTGCTGCTCTG
PRIMER_LEFT_15=656,25
PRIMER_RIGHT_15=1621,20
PRIMER_LEFT_15_TM=64.729
PRIMER_RIGHT_15_TM=56.881
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=966
PRIMER_PAIR_16_PENALTY=13.433020
PRIMER_LEFT_16_PENALTY=9.728547
PRIMER_RIGHT_16_PENALTY=3.704473
PRIMER_LEFT_16_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_16_SEQUENCE=TCTTTTTGTGCTGCTCTGTT
PRIMER_LEFT_16=656,25
PRIMER_RIGHT_16=1619,20
PRIMER_LEFT_16_TM=64.729
PRIMER_RIGHT_16_TM=56.296
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=964
PRIMER_PAIR_17_PENALTY=13.433020
PRIMER_LEFT_17_PENALTY=9.728547
PRIMER_RIGHT_17_PENALTY=3.704473
PRIMER_LEFT_17_SEQUENCE=AGCAGTGAAATGTTGATGCTTTTCC
PRIMER_RIGHT_17_SEQUENCE=TTCTTTTTGTGCTGCTCTGT
PRIMER_LEFT_17=656,25
PRIMER_RIGHT_17=1620,20
PRIMER_LEFT_17_TM=64.729
PRIMER_RIGHT_17_TM=56.296
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=965
=
SEQUENCE_ID=MAGIC.287_3_9014649_9014658_ct-1_hi-0_756826_1505_TRUE
SEQUENCE_TARGET=950,109
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_11792361_11792432_zu-0_wil-2_119356_25065_TRUE
SEQUENCE_TARGET=950,171
SEQUENCE_TEMPLATE=CTAGTCATTGTGATTTAAAATGTTCTCGAGCCTTGTAATTTTCGAGTTCAAGATTGACTTGAAAAGTNGATACCAAATTTCACATGGAAGCAAAATTATTATATACTAATTTCACATGAANCNTATGNNNCAATACCATCAATCTGTTCCCACTTGNCTCGTTATNATAAATTATGATATTGNAAATAAAAGNAAATTNTNNNNAACAGTAGTTTTGTTTTANAGTCNGAAGANNTCAANNANNTTGATCNAACTTGCNACTNNTTNTATNTGAATTCTGCAATGGTTAAANAAAAATANGTCTAGCAAAAATTGAACTNTTTNCTAGACTTNAAATAATTTGCTATGCCTTCGGTTGGTTAAAANCTAGTGATAAGGCAATAATNTNTTTTANTATGTNAAATNNNTTNNTACTANTTTGTTGTTTNAAGCCNNNNNNNNNNAATCCACGNTAAGTNGCATTTNTNNNNTTNNNNTTTTACTNGATGAACATTTGCNNTAGNTGTGNNTTTTACGTTANTAGTATTCTTTTGTCATNTTGCTTTNTATTNGTATGNGAGTTTNGTNGCTATCAAACATGTTTATATNTAAANTNACAATNNAATNGCGAAATTAATTNAATTTNTACAGNNATTNAAATTTTAAATGTTATTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANAAAACTAAAAAANAATAATCATACCGGAATTNNNNNNNNNTTGTTCGGTTTTNGTTNATNCCTATACCNGCTTGGAAANNNNTTTTTTNATTAGTAACCNNTNNNNNNNNNNATTTTCNTATTTTCTCCAATGTTTCCAANTTTTAANANAAACCAAANTTCTGAACCGTATNAAATTTTANNNANTTTNCNAAACTGTTTGNANTATGTCNNNATNTANANAAATNTACCAAAGCTATTGAAAAANTGGAATNTAGTCTCACNNAAACAAAACAAAACTAAATGTANTTTCGATTTTAATTTTGGAACCAATAAANTTANNATAATATTACTANNTGNNNNNNNNATGAAGGGAAATTNNNNNNNNAAAAAAAAAACNATGGCTCAGTTTGTTTAGGTNANTGNTTTNTTTTTTNNTGGTGGGTAAATGAAAAAATATCTCCNAANNTTTTCTTTTCTTACATTTNGNNTTTTTTTTNCCAACCTCTTACAACANNNNNNNNNNAAAATTCAATTTGAGCTTTCACATGGAGTAACGTGGAATCTAGAGTTAGTAGTTAGTACTTTGAAAATATTTTGATATNTGGCNAAACAAAATAGGCAATGGGCTACTCNCTACTAGTGTTGGTAGTACANATTCTACAGAAANATGGAGTAAAGGTAAATAGAATCGATCAGCAAATATGAAATGAAACCTTTCGCTNTTTTGACCTTTTCAGTTTTTTGACATCTAGTTCAAAAATGGCTAAACATTGCATTTATTGAACCTCGCATTTCGGTTCGACATTAGAATTGAGTTACAACATTACTGATCAAACTTTCTGAATANTTACATATATGCCACTATTATNNNNNTAAGTTTTTATTCTATCTNTTTTTTTNTTACAAAAAGATAGTAAAGAAAACTGAAAAACGAATATGATGGCAAATTCAAACTGNTTTTTTCTAACTTTAACGCNTCNNNNNGTCTTTGAGTATGTGTAAATAGAATGGTTGAACNAAATCCNAAATAAATAAATTCCTTTNAAAAAAGNATTATTATATAGAANGGGATTTCAATNNNNNAAANNNNNAAAAGCGTTTTGAANAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTATAAAAGCTTGAGATAGACAACCATGTCCATTTTTACNTTCTATAAAACTTATTTTCTTNTTAACGGTCGATAACTTTACTAATCATTTATCTTTAAGTTTAAAACAATTTTGCTNTAACGTTTAAACTATATCGAATTCGGAATAACTCTATTAACTAAANGTTAACTATTATTAACAATGCGTTTCATTCATCAAAATCAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.125905
PRIMER_LEFT_0_PENALTY=0.095629
PRIMER_RIGHT_0_PENALTY=2.030276
PRIMER_LEFT_0_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_0_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_0=338,20
PRIMER_RIGHT_0=1278,22
PRIMER_LEFT_0_TM=60.096
PRIMER_RIGHT_0_TM=60.030
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=941
PRIMER_PAIR_1_PENALTY=2.949554
PRIMER_LEFT_1_PENALTY=0.095629
PRIMER_RIGHT_1_PENALTY=2.853925
PRIMER_LEFT_1_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_1_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_1=338,20
PRIMER_RIGHT_1=1277,21
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=58.146
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=940
PRIMER_PAIR_2_PENALTY=3.310507
PRIMER_LEFT_2_PENALTY=1.280230
PRIMER_RIGHT_2_PENALTY=2.030276
PRIMER_LEFT_2_SEQUENCE=AATTTGCTATGCCTTCGGTT
PRIMER_RIGHT_2_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_2=337,20
PRIMER_RIGHT_2=1278,22
PRIMER_LEFT_2_TM=58.720
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=942
PRIMER_PAIR_3_PENALTY=4.134156
PRIMER_LEFT_3_PENALTY=1.280230
PRIMER_RIGHT_3_PENALTY=2.853925
PRIMER_LEFT_3_SEQUENCE=AATTTGCTATGCCTTCGGTT
PRIMER_RIGHT_3_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_3=337,20
PRIMER_RIGHT_3=1277,21
PRIMER_LEFT_3_TM=58.720
PRIMER_RIGHT_3_TM=58.146
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=941
PRIMER_PAIR_4_PENALTY=4.349345
PRIMER_LEFT_4_PENALTY=2.319068
PRIMER_RIGHT_4_PENALTY=2.030276
PRIMER_LEFT_4_SEQUENCE=AATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_4_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_4=337,21
PRIMER_RIGHT_4=1278,22
PRIMER_LEFT_4_TM=61.319
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=942
PRIMER_PAIR_5_PENALTY=4.377551
PRIMER_LEFT_5_PENALTY=0.095629
PRIMER_RIGHT_5_PENALTY=4.281923
PRIMER_LEFT_5_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_5_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_5=338,20
PRIMER_RIGHT_5=1279,23
PRIMER_LEFT_5_TM=60.096
PRIMER_RIGHT_5_TM=61.282
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=942
PRIMER_PAIR_6_PENALTY=4.601930
PRIMER_LEFT_6_PENALTY=2.571654
PRIMER_RIGHT_6_PENALTY=2.030276
PRIMER_LEFT_6_SEQUENCE=TAATTTGCTATGCCTTCGGT
PRIMER_RIGHT_6_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_6=336,20
PRIMER_RIGHT_6=1278,22
PRIMER_LEFT_6_TM=57.428
PRIMER_RIGHT_6_TM=60.030
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=943
PRIMER_PAIR_7_PENALTY=4.628572
PRIMER_LEFT_7_PENALTY=0.095629
PRIMER_RIGHT_7_PENALTY=4.532943
PRIMER_LEFT_7_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_7_SEQUENCE=CGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_7=338,20
PRIMER_RIGHT_7=1274,23
PRIMER_LEFT_7_TM=60.096
PRIMER_RIGHT_7_TM=61.533
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=937
PRIMER_PAIR_8_PENALTY=4.840695
PRIMER_LEFT_8_PENALTY=2.810418
PRIMER_RIGHT_8_PENALTY=2.030276
PRIMER_LEFT_8_SEQUENCE=TGCTATGCCTTCGGTTGGTT
PRIMER_RIGHT_8_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_8=341,20
PRIMER_RIGHT_8=1278,22
PRIMER_LEFT_8_TM=62.810
PRIMER_RIGHT_8_TM=60.030
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=45.455
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=938
PRIMER_PAIR_9_PENALTY=4.840695
PRIMER_LEFT_9_PENALTY=2.810418
PRIMER_RIGHT_9_PENALTY=2.030276
PRIMER_LEFT_9_SEQUENCE=TTGCTATGCCTTCGGTTGGT
PRIMER_RIGHT_9_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_9=340,20
PRIMER_RIGHT_9=1278,22
PRIMER_LEFT_9_TM=62.810
PRIMER_RIGHT_9_TM=60.030
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=939
PRIMER_PAIR_10_PENALTY=5.062351
PRIMER_LEFT_10_PENALTY=0.095629
PRIMER_RIGHT_10_PENALTY=4.966722
PRIMER_LEFT_10_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_10_SEQUENCE=GTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_10=338,20
PRIMER_RIGHT_10=1273,22
PRIMER_LEFT_10_TM=60.096
PRIMER_RIGHT_10_TM=57.033
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PAIR_10_PRODUCT_SIZE=936
PRIMER_PAIR_11_PENALTY=5.085712
PRIMER_LEFT_11_PENALTY=3.055436
PRIMER_RIGHT_11_PENALTY=2.030276
PRIMER_LEFT_11_SEQUENCE=ATAATTTGCTATGCCTTCGG
PRIMER_RIGHT_11_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_11=335,20
PRIMER_RIGHT_11=1278,22
PRIMER_LEFT_11_TM=56.945
PRIMER_RIGHT_11_TM=60.030
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=45.455
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.2000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=944
PRIMER_PAIR_12_PENALTY=5.172994
PRIMER_LEFT_12_PENALTY=2.319068
PRIMER_RIGHT_12_PENALTY=2.853925
PRIMER_LEFT_12_SEQUENCE=AATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_12_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_12=337,21
PRIMER_RIGHT_12=1277,21
PRIMER_LEFT_12_TM=61.319
PRIMER_RIGHT_12_TM=58.146
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PAIR_12_PRODUCT_SIZE=941
PRIMER_PAIR_13_PENALTY=5.289002
PRIMER_LEFT_13_PENALTY=3.258726
PRIMER_RIGHT_13_PENALTY=2.030276
PRIMER_LEFT_13_SEQUENCE=TTTGCTATGCCTTCGGTTGG
PRIMER_RIGHT_13_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_13=339,20
PRIMER_RIGHT_13=1278,22
PRIMER_LEFT_13_TM=63.259
PRIMER_RIGHT_13_TM=60.030
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PAIR_13_PRODUCT_SIZE=940
PRIMER_PAIR_14_PENALTY=5.313887
PRIMER_LEFT_14_PENALTY=3.283610
PRIMER_RIGHT_14_PENALTY=2.030276
PRIMER_LEFT_14_SEQUENCE=TAATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_14_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_14=336,22
PRIMER_RIGHT_14=1278,22
PRIMER_LEFT_14_TM=61.284
PRIMER_RIGHT_14_TM=60.030
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=45.455
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=943
PRIMER_PAIR_15_PENALTY=5.425579
PRIMER_LEFT_15_PENALTY=2.571654
PRIMER_RIGHT_15_PENALTY=2.853925
PRIMER_LEFT_15_SEQUENCE=TAATTTGCTATGCCTTCGGT
PRIMER_RIGHT_15_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_15=336,20
PRIMER_RIGHT_15=1277,21
PRIMER_LEFT_15_TM=57.428
PRIMER_RIGHT_15_TM=58.146
PRIMER_LEFT_15_GC_PERCENT=40.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=942
PRIMER_PAIR_16_PENALTY=5.562153
PRIMER_LEFT_16_PENALTY=1.280230
PRIMER_RIGHT_16_PENALTY=4.281923
PRIMER_LEFT_16_SEQUENCE=AATTTGCTATGCCTTCGGTT
PRIMER_RIGHT_16_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_16=337,20
PRIMER_RIGHT_16=1279,23
PRIMER_LEFT_16_TM=58.720
PRIMER_RIGHT_16_TM=61.282
PRIMER_LEFT_16_GC_PERCENT=40.000
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=943
PRIMER_PAIR_17_PENALTY=5.562486
PRIMER_LEFT_17_PENALTY=0.095629
PRIMER_RIGHT_17_PENALTY=5.466858
PRIMER_LEFT_17_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_17_SEQUENCE=ATTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_17=338,20
PRIMER_RIGHT_17=1280,24
PRIMER_LEFT_17_TM=60.096
PRIMER_RIGHT_17_TM=61.467
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=41.667
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=943
PRIMER_PAIR_18_PENALTY=5.664344
PRIMER_LEFT_18_PENALTY=2.810418
PRIMER_RIGHT_18_PENALTY=2.853925
PRIMER_LEFT_18_SEQUENCE=TGCTATGCCTTCGGTTGGTT
PRIMER_RIGHT_18_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_18=341,20
PRIMER_RIGHT_18=1277,21
PRIMER_LEFT_18_TM=62.810
PRIMER_RIGHT_18_TM=58.146
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=937
PRIMER_PAIR_19_PENALTY=5.664344
PRIMER_LEFT_19_PENALTY=2.810418
PRIMER_RIGHT_19_PENALTY=2.853925
PRIMER_LEFT_19_SEQUENCE=TTGCTATGCCTTCGGTTGGT
PRIMER_RIGHT_19_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_19=340,20
PRIMER_RIGHT_19=1277,21
PRIMER_LEFT_19_TM=62.810
PRIMER_RIGHT_19_TM=58.146
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PAIR_19_PRODUCT_SIZE=938
PRIMER_PAIR_20_PENALTY=5.813173
PRIMER_LEFT_20_PENALTY=1.280230
PRIMER_RIGHT_20_PENALTY=4.532943
PRIMER_LEFT_20_SEQUENCE=AATTTGCTATGCCTTCGGTT
PRIMER_RIGHT_20_SEQUENCE=CGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_20=337,20
PRIMER_RIGHT_20=1274,23
PRIMER_LEFT_20_TM=58.720
PRIMER_RIGHT_20_TM=61.533
PRIMER_LEFT_20_GC_PERCENT=40.000
PRIMER_RIGHT_20_GC_PERCENT=43.478
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=9.9000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=938
PRIMER_PAIR_21_PENALTY=5.909361
PRIMER_LEFT_21_PENALTY=3.055436
PRIMER_RIGHT_21_PENALTY=2.853925
PRIMER_LEFT_21_SEQUENCE=ATAATTTGCTATGCCTTCGG
PRIMER_RIGHT_21_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_21=335,20
PRIMER_RIGHT_21=1277,21
PRIMER_LEFT_21_TM=56.945
PRIMER_RIGHT_21_TM=58.146
PRIMER_LEFT_21_GC_PERCENT=40.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=10.2000
PRIMER_RIGHT_21_END_STABILITY=7.0000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=943
PRIMER_PAIR_22_PENALTY=6.112651
PRIMER_LEFT_22_PENALTY=3.258726
PRIMER_RIGHT_22_PENALTY=2.853925
PRIMER_LEFT_22_SEQUENCE=TTTGCTATGCCTTCGGTTGG
PRIMER_RIGHT_22_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_22=339,20
PRIMER_RIGHT_22=1277,21
PRIMER_LEFT_22_TM=63.259
PRIMER_RIGHT_22_TM=58.146
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=939
PRIMER_PAIR_23_PENALTY=6.137536
PRIMER_LEFT_23_PENALTY=3.283610
PRIMER_RIGHT_23_PENALTY=2.853925
PRIMER_LEFT_23_SEQUENCE=TAATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_23_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_23=336,22
PRIMER_RIGHT_23=1277,21
PRIMER_LEFT_23_TM=61.284
PRIMER_RIGHT_23_TM=58.146
PRIMER_LEFT_23_GC_PERCENT=40.909
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=942
PRIMER_PAIR_24_PENALTY=6.246953
PRIMER_LEFT_24_PENALTY=1.280230
PRIMER_RIGHT_24_PENALTY=4.966722
PRIMER_LEFT_24_SEQUENCE=AATTTGCTATGCCTTCGGTT
PRIMER_RIGHT_24_SEQUENCE=GTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_24=337,20
PRIMER_RIGHT_24=1273,22
PRIMER_LEFT_24_TM=58.720
PRIMER_RIGHT_24_TM=57.033
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=40.909
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.9000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=937
PRIMER_PAIR_25_PENALTY=6.317037
PRIMER_LEFT_25_PENALTY=0.095629
PRIMER_RIGHT_25_PENALTY=6.221408
PRIMER_LEFT_25_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_25_SEQUENCE=ACGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_25=338,20
PRIMER_RIGHT_25=1275,24
PRIMER_LEFT_25_TM=60.096
PRIMER_RIGHT_25_TM=62.221
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=41.667
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PAIR_25_PRODUCT_SIZE=938
PRIMER_PAIR_26_PENALTY=6.380008
PRIMER_LEFT_26_PENALTY=4.349731
PRIMER_RIGHT_26_PENALTY=2.030276
PRIMER_LEFT_26_SEQUENCE=ATTTGCTATGCCTTCGGTTGG
PRIMER_RIGHT_26_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_26=338,21
PRIMER_RIGHT_26=1278,22
PRIMER_LEFT_26_TM=63.350
PRIMER_RIGHT_26_TM=60.030
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=7.0000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=941
PRIMER_PAIR_27_PENALTY=6.600991
PRIMER_LEFT_27_PENALTY=2.319068
PRIMER_RIGHT_27_PENALTY=4.281923
PRIMER_LEFT_27_SEQUENCE=AATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_27_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_27=337,21
PRIMER_RIGHT_27=1279,23
PRIMER_LEFT_27_TM=61.319
PRIMER_RIGHT_27_TM=61.282
PRIMER_LEFT_27_GC_PERCENT=42.857
PRIMER_RIGHT_27_GC_PERCENT=43.478
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=943
PRIMER_PAIR_28_PENALTY=6.747088
PRIMER_LEFT_28_PENALTY=1.280230
PRIMER_RIGHT_28_PENALTY=5.466858
PRIMER_LEFT_28_SEQUENCE=AATTTGCTATGCCTTCGGTT
PRIMER_RIGHT_28_SEQUENCE=ATTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_28=337,20
PRIMER_RIGHT_28=1280,24
PRIMER_LEFT_28_TM=58.720
PRIMER_RIGHT_28_TM=61.467
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=41.667
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=944
PRIMER_PAIR_29_PENALTY=6.852011
PRIMER_LEFT_29_PENALTY=2.319068
PRIMER_RIGHT_29_PENALTY=4.532943
PRIMER_LEFT_29_SEQUENCE=AATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_29_SEQUENCE=CGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_29=337,21
PRIMER_RIGHT_29=1274,23
PRIMER_LEFT_29_TM=61.319
PRIMER_RIGHT_29_TM=61.533
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=43.478
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=938
PRIMER_PAIR_30_PENALTY=6.853577
PRIMER_LEFT_30_PENALTY=2.571654
PRIMER_RIGHT_30_PENALTY=4.281923
PRIMER_LEFT_30_SEQUENCE=TAATTTGCTATGCCTTCGGT
PRIMER_RIGHT_30_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_30=336,20
PRIMER_RIGHT_30=1279,23
PRIMER_LEFT_30_TM=57.428
PRIMER_RIGHT_30_TM=61.282
PRIMER_LEFT_30_GC_PERCENT=40.000
PRIMER_RIGHT_30_GC_PERCENT=43.478
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=944
PRIMER_PAIR_31_PENALTY=6.936775
PRIMER_LEFT_31_PENALTY=4.906498
PRIMER_RIGHT_31_PENALTY=2.030276
PRIMER_LEFT_31_SEQUENCE=TTGCTATGCCTTCGGTTGGTT
PRIMER_RIGHT_31_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_31=340,21
PRIMER_RIGHT_31=1278,22
PRIMER_LEFT_31_TM=63.906
PRIMER_RIGHT_31_TM=60.030
PRIMER_LEFT_31_GC_PERCENT=47.619
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.2000
PRIMER_RIGHT_31_END_STABILITY=7.0000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=939
PRIMER_PAIR_32_PENALTY=6.936775
PRIMER_LEFT_32_PENALTY=4.906498
PRIMER_RIGHT_32_PENALTY=2.030276
PRIMER_LEFT_32_SEQUENCE=TTTGCTATGCCTTCGGTTGGT
PRIMER_RIGHT_32_SEQUENCE=TCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_32=339,21
PRIMER_RIGHT_32=1278,22
PRIMER_LEFT_32_TM=63.906
PRIMER_RIGHT_32_TM=60.030
PRIMER_LEFT_32_GC_PERCENT=47.619
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PAIR_32_PRODUCT_SIZE=940
PRIMER_PAIR_33_PENALTY=7.092341
PRIMER_LEFT_33_PENALTY=2.810418
PRIMER_RIGHT_33_PENALTY=4.281923
PRIMER_LEFT_33_SEQUENCE=TGCTATGCCTTCGGTTGGTT
PRIMER_RIGHT_33_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_33=341,20
PRIMER_RIGHT_33=1279,23
PRIMER_LEFT_33_TM=62.810
PRIMER_RIGHT_33_TM=61.282
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=43.478
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=939
PRIMER_PAIR_34_PENALTY=7.092341
PRIMER_LEFT_34_PENALTY=2.810418
PRIMER_RIGHT_34_PENALTY=4.281923
PRIMER_LEFT_34_SEQUENCE=TTGCTATGCCTTCGGTTGGT
PRIMER_RIGHT_34_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_34=340,20
PRIMER_RIGHT_34=1279,23
PRIMER_LEFT_34_TM=62.810
PRIMER_RIGHT_34_TM=61.282
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=43.478
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=7.0000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=940
PRIMER_PAIR_35_PENALTY=7.104597
PRIMER_LEFT_35_PENALTY=2.571654
PRIMER_RIGHT_35_PENALTY=4.532943
PRIMER_LEFT_35_SEQUENCE=TAATTTGCTATGCCTTCGGT
PRIMER_RIGHT_35_SEQUENCE=CGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_35=336,20
PRIMER_RIGHT_35=1274,23
PRIMER_LEFT_35_TM=57.428
PRIMER_RIGHT_35_TM=61.533
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=43.478
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.6000
PRIMER_RIGHT_35_END_STABILITY=7.0000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=939
PRIMER_PAIR_36_PENALTY=7.203657
PRIMER_LEFT_36_PENALTY=4.349731
PRIMER_RIGHT_36_PENALTY=2.853925
PRIMER_LEFT_36_SEQUENCE=ATTTGCTATGCCTTCGGTTGG
PRIMER_RIGHT_36_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_36=338,21
PRIMER_RIGHT_36=1277,21
PRIMER_LEFT_36_TM=63.350
PRIMER_RIGHT_36_TM=58.146
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=3.00
PRIMER_PAIR_36_PRODUCT_SIZE=940
PRIMER_PAIR_37_PENALTY=7.285790
PRIMER_LEFT_37_PENALTY=2.319068
PRIMER_RIGHT_37_PENALTY=4.966722
PRIMER_LEFT_37_SEQUENCE=AATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_37_SEQUENCE=GTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_37=337,21
PRIMER_RIGHT_37=1273,22
PRIMER_LEFT_37_TM=61.319
PRIMER_RIGHT_37_TM=57.033
PRIMER_LEFT_37_GC_PERCENT=42.857
PRIMER_RIGHT_37_GC_PERCENT=40.909
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=3.00
PRIMER_PAIR_37_PRODUCT_SIZE=937
PRIMER_PAIR_38_PENALTY=7.288123
PRIMER_LEFT_38_PENALTY=0.095629
PRIMER_RIGHT_38_PENALTY=7.192495
PRIMER_LEFT_38_SEQUENCE=ATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_38_SEQUENCE=AACTCTAGATTCCACGTTACTCC
PRIMER_LEFT_38=338,20
PRIMER_RIGHT_38=1288,23
PRIMER_LEFT_38_TM=60.096
PRIMER_RIGHT_38_TM=55.808
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=43.478
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.2000
PRIMER_RIGHT_38_END_STABILITY=7.6000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=951
PRIMER_PAIR_39_PENALTY=7.337358
PRIMER_LEFT_39_PENALTY=3.055436
PRIMER_RIGHT_39_PENALTY=4.281923
PRIMER_LEFT_39_SEQUENCE=ATAATTTGCTATGCCTTCGG
PRIMER_RIGHT_39_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_39=335,20
PRIMER_RIGHT_39=1279,23
PRIMER_LEFT_39_TM=56.945
PRIMER_RIGHT_39_TM=61.282
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=43.478
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=10.2000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=945
PRIMER_PAIR_40_PENALTY=7.343362
PRIMER_LEFT_40_PENALTY=2.810418
PRIMER_RIGHT_40_PENALTY=4.532943
PRIMER_LEFT_40_SEQUENCE=TGCTATGCCTTCGGTTGGTT
PRIMER_RIGHT_40_SEQUENCE=CGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_40=341,20
PRIMER_RIGHT_40=1274,23
PRIMER_LEFT_40_TM=62.810
PRIMER_RIGHT_40_TM=61.533
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=43.478
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=934
PRIMER_PAIR_41_PENALTY=7.343362
PRIMER_LEFT_41_PENALTY=2.810418
PRIMER_RIGHT_41_PENALTY=4.532943
PRIMER_LEFT_41_SEQUENCE=TTGCTATGCCTTCGGTTGGT
PRIMER_RIGHT_41_SEQUENCE=CGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_41=340,20
PRIMER_RIGHT_41=1274,23
PRIMER_LEFT_41_TM=62.810
PRIMER_RIGHT_41_TM=61.533
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=43.478
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.2000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=935
PRIMER_PAIR_42_PENALTY=7.501638
PRIMER_LEFT_42_PENALTY=1.280230
PRIMER_RIGHT_42_PENALTY=6.221408
PRIMER_LEFT_42_SEQUENCE=AATTTGCTATGCCTTCGGTT
PRIMER_RIGHT_42_SEQUENCE=ACGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_42=337,20
PRIMER_RIGHT_42=1275,24
PRIMER_LEFT_42_TM=58.720
PRIMER_RIGHT_42_TM=62.221
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=41.667
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=9.9000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=939
PRIMER_PAIR_43_PENALTY=7.538376
PRIMER_LEFT_43_PENALTY=2.571654
PRIMER_RIGHT_43_PENALTY=4.966722
PRIMER_LEFT_43_SEQUENCE=TAATTTGCTATGCCTTCGGT
PRIMER_RIGHT_43_SEQUENCE=GTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_43=336,20
PRIMER_RIGHT_43=1273,22
PRIMER_LEFT_43_TM=57.428
PRIMER_RIGHT_43_TM=57.033
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=40.909
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.6000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=938
PRIMER_PAIR_44_PENALTY=7.540648
PRIMER_LEFT_44_PENALTY=3.258726
PRIMER_RIGHT_44_PENALTY=4.281923
PRIMER_LEFT_44_SEQUENCE=TTTGCTATGCCTTCGGTTGG
PRIMER_RIGHT_44_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_44=339,20
PRIMER_RIGHT_44=1279,23
PRIMER_LEFT_44_TM=63.259
PRIMER_RIGHT_44_TM=61.282
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=43.478
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=7.0000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=941
PRIMER_PAIR_45_PENALTY=7.565533
PRIMER_LEFT_45_PENALTY=3.283610
PRIMER_RIGHT_45_PENALTY=4.281923
PRIMER_LEFT_45_SEQUENCE=TAATTTGCTATGCCTTCGGTTG
PRIMER_RIGHT_45_SEQUENCE=TTCCACGTTACTCCATGTGAAAG
PRIMER_LEFT_45=336,22
PRIMER_RIGHT_45=1279,23
PRIMER_LEFT_45_TM=61.284
PRIMER_RIGHT_45_TM=61.282
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=43.478
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=944
PRIMER_PAIR_46_PENALTY=7.588379
PRIMER_LEFT_46_PENALTY=3.055436
PRIMER_RIGHT_46_PENALTY=4.532943
PRIMER_LEFT_46_SEQUENCE=ATAATTTGCTATGCCTTCGG
PRIMER_RIGHT_46_SEQUENCE=CGTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_46=335,20
PRIMER_RIGHT_46=1274,23
PRIMER_LEFT_46_TM=56.945
PRIMER_RIGHT_46_TM=61.533
PRIMER_LEFT_46_GC_PERCENT=40.000
PRIMER_RIGHT_46_GC_PERCENT=43.478
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=10.2000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=940
PRIMER_PAIR_47_PENALTY=7.760424
PRIMER_LEFT_47_PENALTY=4.906498
PRIMER_RIGHT_47_PENALTY=2.853925
PRIMER_LEFT_47_SEQUENCE=TTGCTATGCCTTCGGTTGGTT
PRIMER_RIGHT_47_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_47=340,21
PRIMER_RIGHT_47=1277,21
PRIMER_LEFT_47_TM=63.906
PRIMER_RIGHT_47_TM=58.146
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=938
PRIMER_PAIR_48_PENALTY=7.760424
PRIMER_LEFT_48_PENALTY=4.906498
PRIMER_RIGHT_48_PENALTY=2.853925
PRIMER_LEFT_48_SEQUENCE=TTTGCTATGCCTTCGGTTGGT
PRIMER_RIGHT_48_SEQUENCE=CCACGTTACTCCATGTGAAAG
PRIMER_LEFT_48=339,21
PRIMER_RIGHT_48=1277,21
PRIMER_LEFT_48_TM=63.906
PRIMER_RIGHT_48_TM=58.146
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=939
PRIMER_PAIR_49_PENALTY=7.777141
PRIMER_LEFT_49_PENALTY=2.810418
PRIMER_RIGHT_49_PENALTY=4.966722
PRIMER_LEFT_49_SEQUENCE=TGCTATGCCTTCGGTTGGTT
PRIMER_RIGHT_49_SEQUENCE=GTTACTCCATGTGAAAGCTCAA
PRIMER_LEFT_49=341,20
PRIMER_RIGHT_49=1273,22
PRIMER_LEFT_49_TM=62.810
PRIMER_RIGHT_49_TM=57.033
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=40.909
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=933
=
SEQUENCE_ID=MAGIC.287_3_14512696_14512703_rsch-4_ct-1_5647_1991619_TRUE
SEQUENCE_TARGET=950,107
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_9010810_9011609_sf-2_edi-0_1008770_4986_TRUE
SEQUENCE_TARGET=950,899
SEQUENCE_TEMPLATE=TGGATAATGCCATGCATTTATTAATNTNTTTGCATGTNTTTTTTNNNTNTTNTTAAAAGGTATAATGTAGNNTATTTGAGTACAATGCATATACCCATATATAGTATNGATNNTATTATATNTTGATGNAANAAATGTGATGNAGCTCAAACTTACAATAATNTTAAGCATGTGATTTTGGCTAATACGTAAANTCAATTTCTATGAATTAAATCTAAGGTTTATATGTTAACAGAATTCTAAAATGAGTACACGTANGTTTCGAAAGATTAATTANTTTNCNTTTATGCCAACCATAANNATAACTNCCAATTTATATTATANTTAANTATCTGCAATGTCTANTTTTTAGAGGAACTACGAATGAAAAATCAAAGAACACATGTATTTTGTGGTCGAAATCTTACCGTAGAAATGATAAAATCAGTTAAAACTTTACGAAATATATACAAGGCAATTGGTAACAATTGTGGTTTATATTAAATATTATANATCTCTATCTACTTCAATTTTAATTTTGTTAATAGATACAAGCTGACTTCTTAAAGTTTAATATATAAANNNNNNNNNNNNNNNNNNNNCCATTTGCCGTACGAGTACATTATCAGTAGNTAATAATCATATTGNTAGCNTCAAATCAGGAAAAGACATCTCATTATGGATNAAACTATATAAGCAATCTAAATATTACACGGACTTATNTCTATTGTAAGCANNNNNNNNNNNCCCCCATGTGCATGTAAACTTATTGATGCTTATCAAGGTTGTCNTANAGAAAATCGAATTCATGAAATAAAGAACTTTTGAATNAAAAATGCGATGGTTACAGTTACATAATTTACAAGCACCCTGAAAAGACAATGTAACTGAAATTTTGATTTGAGAGAAATCAGTNTATCNNAAAAANAAAANAGAGAAANCAATCTATATATGTACTTTATTANTAGAAAAATATAGNATTACATTANAAAGTATATNATANTATATGGAAAAGGTGGGANGTTTAAGGAGGGCATTACTCGAAGCACATTTGTTTTGGCNTTGACTTTGGCATGTAAGACAAGCCACATGGCCGGCCGGTTATCACCGTTAATCAAGACACATGANCATTGCTTNGATTTATTTTNTTTGNTAATACAATTNGNTTTACTTTATAATCCACCGTANTNNNNNNNNNNNAANNATTTTTNGNTGGTTCTTGACTCTATTAGTTTATATTTTTCAGATGAATATTTAATAAGNNATNCACANTTTTTTNNTTTCTGTTTTTANANTNTCGACACGAAAAAATTGCGTCACTCGTCAATCAAAAGNTTGATNTGGAGTTTTATATTTTAAACTATTATATANGTTAAANNAANAANAGNNNTACATTNNTCTTCCNANNNCNTAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_10087445_10087552_zu-0_hi-0_20684_725356_TRUE
SEQUENCE_TARGET=950,207
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_9613768_9613791_bur-0_oy-0_7989_10903_TRUE
SEQUENCE_TARGET=950,123
SEQUENCE_TEMPLATE=GTTTGAGTGGCNCATTTTGAATGNACCGCATAAANACAACCNTTCTTGATGCAATGATANCCCCCGTANTCATTGTTAATCTTCCGGCGGCAAANACCACAAATNCNATCTCCTTTTGGGAAAGAATGGGTAAAANAGATANAATGGNNGTGNCGAGAAATCCGTATGACATGAGGTAAGCTNAAACANCTTTGATGNACGATAAAGTNGCAAGGNGGNCATATATAGAAAANCAANTTTGAATGTTTNAAGGCNCANAGGCTGCAAGTTNNGGNATCCNGACTTGGAAANANAGCAAGTGTATGCNCATGCCACTTGGGTTGGTCGATAGACAAGACTNGTGATTTCTTNGCACATGTAAATTTGAGCCCAAAATCGNAAGNAGAACAAAAATAAAACATACTTGTGGTATCGTCATGGAGAANTTCGTGACAGCAATAGCATTGCNNNANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_5_14883736_14884490_mt-0_wil-2_134545_465503_TRUE
SEQUENCE_TARGET=950,854
SEQUENCE_TEMPLATE=NNNNNNTTTTTNTTTTTTTACTATNNNNNNTAAAAATATTAACCTNACAATATTACTTCTACCGTTGACTTTTTTTGTNTTACNAATCAGAATNTTACAAAGGCACCAGCAAAAGAGAACAAAAAACCTCTCGAGTTCAAGCTTCATTCTGGCGAGAGAGCAGTGAAACGTGCAATGTTCAACTATTCGGTTTACACANNCTTCCTCNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNATTCATCATCTTGGCAGGTTGCAACTAATTATTANATCCAGAAACTTCAGAAAAAACAAGAAGAGAGGTTGCAAAAGGTAAATTACAAAAGAAAAAAAATGGTTTACTATTATTTGGCTTGANAACANCCNNAAACAATTTAAACCNACACACACCGTANTCTGNTTGGTCTTGGACTCTTGGTCTCTCTTTGGCTGTAAATTTACGACGACTAAACATAACTAGAANCTCTCTATTTATATGAATCATGCTTGTCTAAAATCGAACATTTCACTTTCATGACACCAAGACCACAAGTAGATATAGAACGGAANCTACTCTAACTAAAACAGTGAGAAATTTTGGAATTTGGTATCCCTTGGTTTGAGAAGAATACTCGAAGTTAAGCCGCGTTATTAAATCGAACCATATGTAACAGATGATAGAAGAGGAAGAGATTCGNATGTTGAGGAAGGAAATGGTTCCAAAAGCTCAATTGATGCCTTTCTTCGACAGACCTTTTCTCCCACAAAGGTCAGTTTATAGTTTACAAAANTCTAGTTTCAANCTATCTATAGTTTATTCTATANTCAAAGAGATAAATTTAGNTAGTATATTAACAAATTGTATAATAGAAACTTTCTGAAATAATATGATATTGTTATGTAACTAATTTATTTTATGNTGAAATAAATATNTGGGTGTATAGATCGAGCAGACCATTGACGATGCCAAAAGAACCAAGCTTTGGAAACGTAAACAGCACNTGTTGGACTTGTGTTTTCAACAACCAACACTATCTTTACCATATTAACNATGCTCATGCTTAAATCNCGAGTAACTAGCTCACATAGAGGCTTTTTTCTAATTTTCGTTTCAAATCTTTTTTTCAGTTTACTTTTATTTTTGTTCTTGGCAGCTTTTGTCTTTTTCTCTTCCCCTCTTCNTTTTTTTCAGTATAATATTCTGTAATTTTTTTCGTAGATTAAATAAACGAGTAAATNCTGATTTGAAGTACTATTGGGTCTGATTTGAAGTAGACAATATANTNTGCCATTCTCCATTNNNNNNTCTAANCCTNNNCAAATAANCNGAANCGAATGTANCAAACCAAAACCGAANNAAAAGAACCAAAAATTGAAAACTAANCAAATAGATACTAAATTNTATAACCGAAACCGAATNGATATAAACNGAAATATTTTGGATAANCAAAAATATNCGAAATATAAATATATTTTTGAAAATATTAGTTATTTNTACACTNAANAATTAAAAATACTCGAGAATATATATAAACTTAGTAAAAATACTCGACAAAAACGAGTGCCGGAAAGGAGCAGAGANAGGAGGAAACACATAACCAGGGTTGAAAGCCAAATTCAGAGAGAAAAGATTCCAGAGCTGTTACATCCCCAAATCAATGAGTGATAGATAGAGGAAAACAAAAAAAAAGGTTTTTGGAGTTACATCTCCAAATGTCTGTGTTTGAACAATGTCATATTGTTTACACGAAAGAGNTGTTAAAGAGCAATATTAATCTAGGAAGGTTCTCAAGAGCCACTGTGAAGAGGCCACCAAGAATAAACATCAAAACAAATGCAAAAAATATCCTCCCAATGTATTTCAGACTTTGCTCTCCGATTTCTGGTGNTGAATCNTTTGCAAAACCCTGCAAAGCAAACCNCAAAACACTTCAGCATTTTCCCACAATCNGATAAACTACAGGGAAATCACATAACAAACGCCAAGTNATATGAAACCAACACCATAATCTCAAGNAAGAAGAAGTTTACAGTTATTTTACANTCTACCAAGTCAACTGTTTCAACATTCTTCTGTTCCTTATCGAGCTCATTCTCTATATTCTAACAGACGTATGGTTCTAGTTTTTCTACACTCCTACTTAGGCTTTGACAANGAAAAGTATTCNAAGTATAAGTTAGATACTAAANTGTAATCATGCTAGCATCAACTATTCAGATATTTATAACACAATANCACACTATGAAGCTACTAACAAACTCAAGATACTTACCGTCGAAAATACAGAAGAAAACCGCTGTTGTCGATTGTTTTTCTCCGTTCCTGCATCAAAGAGAAAAGATTGGCATTAGAGTACATGAACCAAAGGTAAAGAGCTACATAANGCAGTTTGACAAGAATTAAATTACCAGGTAGATCAGCATCAACAACTGTCTTCAACTTCCAACCAAGTCCATAATTCAGAAGACTAGCANTATCACCAAAACTAATAGGCTCTTCACCTTCCTTTGNTGCTTCGATGTAAATCACTCGAGGTTTCAACGAACCAAGTTTCCTGTTGATCACAGAGACAGCAAACTCCGCCTTTGTCCCTGTTGTGAATCGGTAGTCTTTACCAGAAACTTCACAAAAAACCTCCAGAAACTAGTATACCACAATNAACAATGTTTTAGTTNACCAAAAACAACACTAGTATAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAATCAACGAGTTAAGGA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_4_4445983_4446174_tsu-0_wil-2_65625_3111_TRUE
SEQUENCE_TARGET=950,291
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGTTNNNGATGACAACACACTTCTCCTTCTTCCTCTTTNAGANAAACTTAATCTCTATCTTNNTTCTCCTTNGACCAGGACCTCAATACATGAGTCATGAAAGCTCCTGCAAAGCTTCAGCTCCAACNNATCTCGTTTGCACCATCAAACAACANAAACTCATTACTATTTACTTCAAATAAAACTGTCNNGGNAACATACTCAAAANTNAGGNNCTAACCNTTAGAAACGTGATCCNACCGTTCAATATGTANCTAGTAGAATGATGAATAACTCCACNAAGTTTCATCCCGATCAGACATCACATGAATCTCTCACGTTCTTNTGAAGATGATGCATCAGAACTGATANTTAGGNTTCTGCAACTTGAGATGATTCTGGGCNAACTTCCAAGGTCAATTCNGGATGGAGCTTAANTCCGATGNACAAGNTGTCAGATACNCTGANTTCCTTGAAGCAANNTCTTTCTATAGNNGNNNNNNNNNNNNNNNNATTCCTTTTGGTCCATNGGGAAAACGCATCACAAAACCAGTTGTCAAACACTGTTCTNGAAANNTGGGAACGAGATCTATTCAGAACCGTTAAGAACTCGCCCAAACTCCTTNGNACGTGACGGGAAACGGATCACCAATTCCACCACTTGNACCNTTTCCTTCTTTCACATATNCACNNNNAAACCCAAAAGNTAACTTGATNTATCTGCCNCAAATTCAATGNCTAAGAGAGACAATTCCANTANAAAGAAGGCATAAATAGATTAGGTTTTCCTTACCCAATCAAGCCGTTGTTTAACGNTGATGATCCGTGACGTTCNCTTTCACTCCACACTTGTNCGTGATCANANANNAAACNACATGCTCCAACAACTCCAATCTCAANGTCAANGGTGATGGTNGCTGATTTAGTTTANGGNTTAAGATACCCCAATANATATNACANTTACAAAANCNTTNTTNNNNNNNNNNNNNNNNNGATTGGGCCATTTATGGGCNATANCATNNNNTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGATTTATNTCCANATAAAANCACTTACTTGTATCTNNNNNNAACACCACNTCCACATTNACGNTATNCAAGCAGACNACCNTTCATTAANACTTNTANCTTACCGTCTTTACAAATCGACACNTGCTTTCTAACNATGGTANCTCTTTTGATGCGCCACTCTTGTAGCNTANANAANCTTTAANNNNNACTTTTCAACATCCACTGNACNTAGTNCACAAANAAACATTNTTNTCTAGTACCATAACAACANAAGATGAAANTNTCNTTTACCTTGATATGANTTCTCATANTCTTAAATATGCTTATTGACANGGNTTCCTTCCCATTNTNTTTTTTCCACNNTTTCTAGGGCNCCTTCCCCATCCTTGTGAANTTAACAAGGTTANATGTCTATTGACTTAGACGGTTTCCATNTGTCTTACCTTAAATGTCTCTGAGCTCTTATTCTCTTNTAATACNTTCTTNCATNTNNTTTNTCNATAACCTTANCCTTTAGCCTTTCATGACTATCNTGGTANACTGANNCTTACTTGACANTTCTTAACGCCNTACTAAAGCGAATAGAACACGAGAATCCNNATTACATTTCGAGAATCACAAGAAATGTAATTAAGTCTTCCCAAAAAGAAACTAATTAANNNNATATATAAAAATCACTTAACAAAAGATTTACTCAACGCAATCAAAGAAATCAATCATCAAGCCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.258025
PRIMER_LEFT_0_PENALTY=0.246726
PRIMER_RIGHT_0_PENALTY=0.011300
PRIMER_LEFT_0_SEQUENCE=AGAACTCGCCCAAACTCCTT
PRIMER_RIGHT_0_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_0=637,20
PRIMER_RIGHT_0=1308,20
PRIMER_LEFT_0_TM=60.247
PRIMER_RIGHT_0_TM=60.011
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=6.9000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=672
PRIMER_PAIR_1_PENALTY=0.258025
PRIMER_LEFT_1_PENALTY=0.246726
PRIMER_RIGHT_1_PENALTY=0.011300
PRIMER_LEFT_1_SEQUENCE=AAGAACTCGCCCAAACTCCT
PRIMER_RIGHT_1_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_1=636,20
PRIMER_RIGHT_1=1308,20
PRIMER_LEFT_1_TM=60.247
PRIMER_RIGHT_1_TM=60.011
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=673
PRIMER_PAIR_2_PENALTY=0.264782
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.011300
PRIMER_LEFT_2_SEQUENCE=CGTTAAGAACTCGCCCAAAC
PRIMER_RIGHT_2_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_2=632,20
PRIMER_RIGHT_2=1308,20
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.011
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=677
PRIMER_PAIR_3_PENALTY=0.358618
PRIMER_LEFT_3_PENALTY=0.347318
PRIMER_RIGHT_3_PENALTY=0.011300
PRIMER_LEFT_3_SEQUENCE=AAACGGATCACCAATTCCAC
PRIMER_RIGHT_3_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_3=670,20
PRIMER_RIGHT_3=1308,20
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=60.011
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=639
PRIMER_PAIR_4_PENALTY=0.363553
PRIMER_LEFT_4_PENALTY=0.352253
PRIMER_RIGHT_4_PENALTY=0.011300
PRIMER_LEFT_4_SEQUENCE=GGAAAACGCATCACAAAACC
PRIMER_RIGHT_4_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_4=563,20
PRIMER_RIGHT_4=1308,20
PRIMER_LEFT_4_TM=60.352
PRIMER_RIGHT_4_TM=60.011
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=746
PRIMER_PAIR_5_PENALTY=0.363553
PRIMER_LEFT_5_PENALTY=0.352253
PRIMER_RIGHT_5_PENALTY=0.011300
PRIMER_LEFT_5_SEQUENCE=GGGAAAACGCATCACAAAAC
PRIMER_RIGHT_5_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_5=562,20
PRIMER_RIGHT_5=1308,20
PRIMER_LEFT_5_TM=60.352
PRIMER_RIGHT_5_TM=60.011
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=747
PRIMER_PAIR_6_PENALTY=0.375053
PRIMER_LEFT_6_PENALTY=0.363753
PRIMER_RIGHT_6_PENALTY=0.011300
PRIMER_LEFT_6_SEQUENCE=GGATCACCAATTCCACCACT
PRIMER_RIGHT_6_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_6=674,20
PRIMER_RIGHT_6=1308,20
PRIMER_LEFT_6_TM=59.636
PRIMER_RIGHT_6_TM=60.011
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=635
PRIMER_PAIR_7_PENALTY=0.376470
PRIMER_LEFT_7_PENALTY=0.365170
PRIMER_RIGHT_7_PENALTY=0.011300
PRIMER_LEFT_7_SEQUENCE=TTACCCAATCAAGCCGTTGT
PRIMER_RIGHT_7_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_7=821,20
PRIMER_RIGHT_7=1308,20
PRIMER_LEFT_7_TM=60.365
PRIMER_RIGHT_7_TM=60.011
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=488
PRIMER_PAIR_8_PENALTY=0.444912
PRIMER_LEFT_8_PENALTY=0.433612
PRIMER_RIGHT_8_PENALTY=0.011300
PRIMER_LEFT_8_SEQUENCE=GAAAACGCATCACAAAACCA
PRIMER_RIGHT_8_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_8=564,20
PRIMER_RIGHT_8=1308,20
PRIMER_LEFT_8_TM=59.566
PRIMER_RIGHT_8_TM=60.011
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=745
PRIMER_PAIR_9_PENALTY=0.507654
PRIMER_LEFT_9_PENALTY=0.496354
PRIMER_RIGHT_9_PENALTY=0.011300
PRIMER_LEFT_9_SEQUENCE=CATCCCGATCAGACATCACA
PRIMER_RIGHT_9_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_9=339,20
PRIMER_RIGHT_9=1308,20
PRIMER_LEFT_9_TM=60.496
PRIMER_RIGHT_9_TM=60.011
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=970
PRIMER_PAIR_10_PENALTY=0.580693
PRIMER_LEFT_10_PENALTY=0.569393
PRIMER_RIGHT_10_PENALTY=0.011300
PRIMER_LEFT_10_SEQUENCE=TCATCCCGATCAGACATCAC
PRIMER_RIGHT_10_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_10=338,20
PRIMER_RIGHT_10=1308,20
PRIMER_LEFT_10_TM=59.431
PRIMER_RIGHT_10_TM=60.011
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.3000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=971
PRIMER_PAIR_11_PENALTY=0.648152
PRIMER_LEFT_11_PENALTY=0.246726
PRIMER_RIGHT_11_PENALTY=0.401426
PRIMER_LEFT_11_SEQUENCE=AGAACTCGCCCAAACTCCTT
PRIMER_RIGHT_11_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_11=637,20
PRIMER_RIGHT_11=1307,20
PRIMER_LEFT_11_TM=60.247
PRIMER_RIGHT_11_TM=60.401
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=671
PRIMER_PAIR_12_PENALTY=0.648152
PRIMER_LEFT_12_PENALTY=0.246726
PRIMER_RIGHT_12_PENALTY=0.401426
PRIMER_LEFT_12_SEQUENCE=AAGAACTCGCCCAAACTCCT
PRIMER_RIGHT_12_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_12=636,20
PRIMER_RIGHT_12=1307,20
PRIMER_LEFT_12_TM=60.247
PRIMER_RIGHT_12_TM=60.401
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=672
PRIMER_PAIR_13_PENALTY=0.654908
PRIMER_LEFT_13_PENALTY=0.253482
PRIMER_RIGHT_13_PENALTY=0.401426
PRIMER_LEFT_13_SEQUENCE=CGTTAAGAACTCGCCCAAAC
PRIMER_RIGHT_13_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_13=632,20
PRIMER_RIGHT_13=1307,20
PRIMER_LEFT_13_TM=59.747
PRIMER_RIGHT_13_TM=60.401
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=676
PRIMER_PAIR_14_PENALTY=0.699263
PRIMER_LEFT_14_PENALTY=0.687963
PRIMER_RIGHT_14_PENALTY=0.011300
PRIMER_LEFT_14_SEQUENCE=TAAGAACTCGCCCAAACTCC
PRIMER_RIGHT_14_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_14=635,20
PRIMER_RIGHT_14=1308,20
PRIMER_LEFT_14_TM=59.312
PRIMER_RIGHT_14_TM=60.011
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=674
PRIMER_PAIR_15_PENALTY=0.713459
PRIMER_LEFT_15_PENALTY=0.702159
PRIMER_RIGHT_15_PENALTY=0.011300
PRIMER_LEFT_15_SEQUENCE=GAAACGGATCACCAATTCCA
PRIMER_RIGHT_15_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_15=669,20
PRIMER_RIGHT_15=1308,20
PRIMER_LEFT_15_TM=60.702
PRIMER_RIGHT_15_TM=60.011
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=640
PRIMER_PAIR_16_PENALTY=0.748744
PRIMER_LEFT_16_PENALTY=0.347318
PRIMER_RIGHT_16_PENALTY=0.401426
PRIMER_LEFT_16_SEQUENCE=AAACGGATCACCAATTCCAC
PRIMER_RIGHT_16_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_16=670,20
PRIMER_RIGHT_16=1307,20
PRIMER_LEFT_16_TM=59.653
PRIMER_RIGHT_16_TM=60.401
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=638
PRIMER_PAIR_17_PENALTY=0.753679
PRIMER_LEFT_17_PENALTY=0.352253
PRIMER_RIGHT_17_PENALTY=0.401426
PRIMER_LEFT_17_SEQUENCE=GGAAAACGCATCACAAAACC
PRIMER_RIGHT_17_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_17=563,20
PRIMER_RIGHT_17=1307,20
PRIMER_LEFT_17_TM=60.352
PRIMER_RIGHT_17_TM=60.401
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=745
PRIMER_PAIR_18_PENALTY=0.753679
PRIMER_LEFT_18_PENALTY=0.352253
PRIMER_RIGHT_18_PENALTY=0.401426
PRIMER_LEFT_18_SEQUENCE=GGGAAAACGCATCACAAAAC
PRIMER_RIGHT_18_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_18=562,20
PRIMER_RIGHT_18=1307,20
PRIMER_LEFT_18_TM=60.352
PRIMER_RIGHT_18_TM=60.401
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=746
PRIMER_PAIR_19_PENALTY=0.765179
PRIMER_LEFT_19_PENALTY=0.363753
PRIMER_RIGHT_19_PENALTY=0.401426
PRIMER_LEFT_19_SEQUENCE=GGATCACCAATTCCACCACT
PRIMER_RIGHT_19_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_19=674,20
PRIMER_RIGHT_19=1307,20
PRIMER_LEFT_19_TM=59.636
PRIMER_RIGHT_19_TM=60.401
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=634
PRIMER_PAIR_20_PENALTY=0.766596
PRIMER_LEFT_20_PENALTY=0.365170
PRIMER_RIGHT_20_PENALTY=0.401426
PRIMER_LEFT_20_SEQUENCE=TTACCCAATCAAGCCGTTGT
PRIMER_RIGHT_20_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_20=821,20
PRIMER_RIGHT_20=1307,20
PRIMER_LEFT_20_TM=60.365
PRIMER_RIGHT_20_TM=60.401
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=7.3000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=487
PRIMER_PAIR_21_PENALTY=0.784809
PRIMER_LEFT_21_PENALTY=0.246726
PRIMER_RIGHT_21_PENALTY=0.538083
PRIMER_LEFT_21_SEQUENCE=AAGAACTCGCCCAAACTCCT
PRIMER_RIGHT_21_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_21=636,20
PRIMER_RIGHT_21=1303,20
PRIMER_LEFT_21_TM=60.247
PRIMER_RIGHT_21_TM=60.538
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.9000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PAIR_21_PRODUCT_SIZE=668
PRIMER_PAIR_22_PENALTY=0.791566
PRIMER_LEFT_22_PENALTY=0.253482
PRIMER_RIGHT_22_PENALTY=0.538083
PRIMER_LEFT_22_SEQUENCE=CGTTAAGAACTCGCCCAAAC
PRIMER_RIGHT_22_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_22=632,20
PRIMER_RIGHT_22=1303,20
PRIMER_LEFT_22_TM=59.747
PRIMER_RIGHT_22_TM=60.538
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=672
PRIMER_PAIR_23_PENALTY=0.835038
PRIMER_LEFT_23_PENALTY=0.433612
PRIMER_RIGHT_23_PENALTY=0.401426
PRIMER_LEFT_23_SEQUENCE=GAAAACGCATCACAAAACCA
PRIMER_RIGHT_23_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_23=564,20
PRIMER_RIGHT_23=1307,20
PRIMER_LEFT_23_TM=59.566
PRIMER_RIGHT_23_TM=60.401
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.3000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=744
PRIMER_PAIR_24_PENALTY=0.872463
PRIMER_LEFT_24_PENALTY=0.861164
PRIMER_RIGHT_24_PENALTY=0.011300
PRIMER_LEFT_24_SEQUENCE=ACATGCTCCAACAACTCCAA
PRIMER_RIGHT_24_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_24=905,20
PRIMER_RIGHT_24=1308,20
PRIMER_LEFT_24_TM=59.139
PRIMER_RIGHT_24_TM=60.011
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=404
PRIMER_PAIR_25_PENALTY=0.885402
PRIMER_LEFT_25_PENALTY=0.347318
PRIMER_RIGHT_25_PENALTY=0.538083
PRIMER_LEFT_25_SEQUENCE=AAACGGATCACCAATTCCAC
PRIMER_RIGHT_25_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_25=670,20
PRIMER_RIGHT_25=1303,20
PRIMER_LEFT_25_TM=59.653
PRIMER_RIGHT_25_TM=60.538
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=634
PRIMER_PAIR_26_PENALTY=0.890337
PRIMER_LEFT_26_PENALTY=0.352253
PRIMER_RIGHT_26_PENALTY=0.538083
PRIMER_LEFT_26_SEQUENCE=GGAAAACGCATCACAAAACC
PRIMER_RIGHT_26_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_26=563,20
PRIMER_RIGHT_26=1303,20
PRIMER_LEFT_26_TM=60.352
PRIMER_RIGHT_26_TM=60.538
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.7000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=741
PRIMER_PAIR_27_PENALTY=0.890337
PRIMER_LEFT_27_PENALTY=0.352253
PRIMER_RIGHT_27_PENALTY=0.538083
PRIMER_LEFT_27_SEQUENCE=GGGAAAACGCATCACAAAAC
PRIMER_RIGHT_27_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_27=562,20
PRIMER_RIGHT_27=1303,20
PRIMER_LEFT_27_TM=60.352
PRIMER_RIGHT_27_TM=60.538
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.0000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=742
PRIMER_PAIR_28_PENALTY=0.897780
PRIMER_LEFT_28_PENALTY=0.496354
PRIMER_RIGHT_28_PENALTY=0.401426
PRIMER_LEFT_28_SEQUENCE=CATCCCGATCAGACATCACA
PRIMER_RIGHT_28_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_28=339,20
PRIMER_RIGHT_28=1307,20
PRIMER_LEFT_28_TM=60.496
PRIMER_RIGHT_28_TM=60.401
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.3000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=969
PRIMER_PAIR_29_PENALTY=0.901837
PRIMER_LEFT_29_PENALTY=0.363753
PRIMER_RIGHT_29_PENALTY=0.538083
PRIMER_LEFT_29_SEQUENCE=GGATCACCAATTCCACCACT
PRIMER_RIGHT_29_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_29=674,20
PRIMER_RIGHT_29=1303,20
PRIMER_LEFT_29_TM=59.636
PRIMER_RIGHT_29_TM=60.538
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=630
PRIMER_PAIR_30_PENALTY=0.903254
PRIMER_LEFT_30_PENALTY=0.365170
PRIMER_RIGHT_30_PENALTY=0.538083
PRIMER_LEFT_30_SEQUENCE=TTACCCAATCAAGCCGTTGT
PRIMER_RIGHT_30_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_30=821,20
PRIMER_RIGHT_30=1303,20
PRIMER_LEFT_30_TM=60.365
PRIMER_RIGHT_30_TM=60.538
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=483
PRIMER_PAIR_31_PENALTY=0.970819
PRIMER_LEFT_31_PENALTY=0.569393
PRIMER_RIGHT_31_PENALTY=0.401426
PRIMER_LEFT_31_SEQUENCE=TCATCCCGATCAGACATCAC
PRIMER_RIGHT_31_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_31=338,20
PRIMER_RIGHT_31=1307,20
PRIMER_LEFT_31_TM=59.431
PRIMER_RIGHT_31_TM=60.401
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.3000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=970
PRIMER_PAIR_32_PENALTY=0.971695
PRIMER_LEFT_32_PENALTY=0.433612
PRIMER_RIGHT_32_PENALTY=0.538083
PRIMER_LEFT_32_SEQUENCE=GAAAACGCATCACAAAACCA
PRIMER_RIGHT_32_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_32=564,20
PRIMER_RIGHT_32=1303,20
PRIMER_LEFT_32_TM=59.566
PRIMER_RIGHT_32_TM=60.538
PRIMER_LEFT_32_GC_PERCENT=40.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=740
PRIMER_PAIR_33_PENALTY=0.991983
PRIMER_LEFT_33_PENALTY=0.980684
PRIMER_RIGHT_33_PENALTY=0.011300
PRIMER_LEFT_33_SEQUENCE=TTTCCTTACCCAATCAAGCC
PRIMER_RIGHT_33_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_33=816,20
PRIMER_RIGHT_33=1308,20
PRIMER_LEFT_33_TM=59.019
PRIMER_RIGHT_33_TM=60.011
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=6.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=493
PRIMER_PAIR_34_PENALTY=0.998326
PRIMER_LEFT_34_PENALTY=0.987026
PRIMER_RIGHT_34_PENALTY=0.011300
PRIMER_LEFT_34_SEQUENCE=GAACCGTTAAGAACTCGCCC
PRIMER_RIGHT_34_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_34=628,20
PRIMER_RIGHT_34=1308,20
PRIMER_LEFT_34_TM=60.987
PRIMER_RIGHT_34_TM=60.011
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=12.9000
PRIMER_RIGHT_34_END_STABILITY=6.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=681
PRIMER_PAIR_35_PENALTY=1.034438
PRIMER_LEFT_35_PENALTY=0.496354
PRIMER_RIGHT_35_PENALTY=0.538083
PRIMER_LEFT_35_SEQUENCE=CATCCCGATCAGACATCACA
PRIMER_RIGHT_35_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_35=339,20
PRIMER_RIGHT_35=1303,20
PRIMER_LEFT_35_TM=60.496
PRIMER_RIGHT_35_TM=60.538
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=965
PRIMER_PAIR_36_PENALTY=1.089389
PRIMER_LEFT_36_PENALTY=0.687963
PRIMER_RIGHT_36_PENALTY=0.401426
PRIMER_LEFT_36_SEQUENCE=TAAGAACTCGCCCAAACTCC
PRIMER_RIGHT_36_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_36=635,20
PRIMER_RIGHT_36=1307,20
PRIMER_LEFT_36_TM=59.312
PRIMER_RIGHT_36_TM=60.401
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.6000
PRIMER_RIGHT_36_END_STABILITY=7.3000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=673
PRIMER_PAIR_37_PENALTY=1.103585
PRIMER_LEFT_37_PENALTY=0.702159
PRIMER_RIGHT_37_PENALTY=0.401426
PRIMER_LEFT_37_SEQUENCE=GAAACGGATCACCAATTCCA
PRIMER_RIGHT_37_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_37=669,20
PRIMER_RIGHT_37=1307,20
PRIMER_LEFT_37_TM=60.702
PRIMER_RIGHT_37_TM=60.401
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.5000
PRIMER_RIGHT_37_END_STABILITY=7.3000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=639
PRIMER_PAIR_38_PENALTY=1.107477
PRIMER_LEFT_38_PENALTY=0.569393
PRIMER_RIGHT_38_PENALTY=0.538083
PRIMER_LEFT_38_SEQUENCE=TCATCCCGATCAGACATCAC
PRIMER_RIGHT_38_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_38=338,20
PRIMER_RIGHT_38=1303,20
PRIMER_LEFT_38_TM=59.431
PRIMER_RIGHT_38_TM=60.538
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.3000
PRIMER_RIGHT_38_END_STABILITY=6.7000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PAIR_38_PRODUCT_SIZE=966
PRIMER_PAIR_39_PENALTY=1.160969
PRIMER_LEFT_39_PENALTY=0.246726
PRIMER_RIGHT_39_PENALTY=0.914244
PRIMER_LEFT_39_SEQUENCE=AGAACTCGCCCAAACTCCTT
PRIMER_RIGHT_39_SEQUENCE=AAGAGTGGCGCATCAAAAGA
PRIMER_LEFT_39=637,20
PRIMER_RIGHT_39=1304,20
PRIMER_LEFT_39_TM=60.247
PRIMER_RIGHT_39_TM=60.914
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=45.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.2000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=668
PRIMER_PAIR_40_PENALTY=1.160969
PRIMER_LEFT_40_PENALTY=0.246726
PRIMER_RIGHT_40_PENALTY=0.914244
PRIMER_LEFT_40_SEQUENCE=AAGAACTCGCCCAAACTCCT
PRIMER_RIGHT_40_SEQUENCE=AAGAGTGGCGCATCAAAAGA
PRIMER_LEFT_40=636,20
PRIMER_RIGHT_40=1304,20
PRIMER_LEFT_40_TM=60.247
PRIMER_RIGHT_40_TM=60.914
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=669
PRIMER_PAIR_41_PENALTY=1.167726
PRIMER_LEFT_41_PENALTY=0.253482
PRIMER_RIGHT_41_PENALTY=0.914244
PRIMER_LEFT_41_SEQUENCE=CGTTAAGAACTCGCCCAAAC
PRIMER_RIGHT_41_SEQUENCE=AAGAGTGGCGCATCAAAAGA
PRIMER_LEFT_41=632,20
PRIMER_RIGHT_41=1304,20
PRIMER_LEFT_41_TM=59.747
PRIMER_RIGHT_41_TM=60.914
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=673
PRIMER_PAIR_42_PENALTY=1.226047
PRIMER_LEFT_42_PENALTY=0.687963
PRIMER_RIGHT_42_PENALTY=0.538083
PRIMER_LEFT_42_SEQUENCE=TAAGAACTCGCCCAAACTCC
PRIMER_RIGHT_42_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_42=635,20
PRIMER_RIGHT_42=1303,20
PRIMER_LEFT_42_TM=59.312
PRIMER_RIGHT_42_TM=60.538
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=2.00
PRIMER_PAIR_42_PRODUCT_SIZE=669
PRIMER_PAIR_43_PENALTY=1.228500
PRIMER_LEFT_43_PENALTY=1.217201
PRIMER_RIGHT_43_PENALTY=0.011300
PRIMER_LEFT_43_SEQUENCE=CCTTACCCAATCAAGCCGTT
PRIMER_RIGHT_43_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_43=819,20
PRIMER_RIGHT_43=1308,20
PRIMER_LEFT_43_TM=61.217
PRIMER_RIGHT_43_TM=60.011
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=9.9000
PRIMER_RIGHT_43_END_STABILITY=6.9000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=490
PRIMER_PAIR_44_PENALTY=1.240243
PRIMER_LEFT_44_PENALTY=0.702159
PRIMER_RIGHT_44_PENALTY=0.538083
PRIMER_LEFT_44_SEQUENCE=GAAACGGATCACCAATTCCA
PRIMER_RIGHT_44_SEQUENCE=AGAGTGGCGCATCAAAAGAG
PRIMER_LEFT_44=669,20
PRIMER_RIGHT_44=1303,20
PRIMER_LEFT_44_TM=60.702
PRIMER_RIGHT_44_TM=60.538
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.5000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=635
PRIMER_PAIR_45_PENALTY=1.258865
PRIMER_LEFT_45_PENALTY=1.247566
PRIMER_RIGHT_45_PENALTY=0.011300
PRIMER_LEFT_45_SEQUENCE=GCTCCAACAACTCCAATCTCA
PRIMER_RIGHT_45_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_45=909,21
PRIMER_RIGHT_45=1308,20
PRIMER_LEFT_45_TM=60.248
PRIMER_RIGHT_45_TM=60.011
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.7000
PRIMER_RIGHT_45_END_STABILITY=6.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=400
PRIMER_PAIR_46_PENALTY=1.258865
PRIMER_LEFT_46_PENALTY=1.247566
PRIMER_RIGHT_46_PENALTY=0.011300
PRIMER_LEFT_46_SEQUENCE=TGCTCCAACAACTCCAATCTC
PRIMER_RIGHT_46_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_46=908,21
PRIMER_RIGHT_46=1308,20
PRIMER_LEFT_46_TM=60.248
PRIMER_RIGHT_46_TM=60.011
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=6.3000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=401
PRIMER_PAIR_47_PENALTY=1.259324
PRIMER_LEFT_47_PENALTY=1.248025
PRIMER_RIGHT_47_PENALTY=0.011300
PRIMER_LEFT_47_SEQUENCE=TAAGAACTCGCCCAAACTCCT
PRIMER_RIGHT_47_SEQUENCE=CTACAAGAGTGGCGCATCAA
PRIMER_LEFT_47=635,21
PRIMER_RIGHT_47=1308,20
PRIMER_LEFT_47_TM=60.248
PRIMER_RIGHT_47_TM=60.011
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=674
PRIMER_PAIR_48_PENALTY=1.261562
PRIMER_LEFT_48_PENALTY=0.347318
PRIMER_RIGHT_48_PENALTY=0.914244
PRIMER_LEFT_48_SEQUENCE=AAACGGATCACCAATTCCAC
PRIMER_RIGHT_48_SEQUENCE=AAGAGTGGCGCATCAAAAGA
PRIMER_LEFT_48=670,20
PRIMER_RIGHT_48=1304,20
PRIMER_LEFT_48_TM=59.653
PRIMER_RIGHT_48_TM=60.914
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=635
PRIMER_PAIR_49_PENALTY=1.262589
PRIMER_LEFT_49_PENALTY=0.861164
PRIMER_RIGHT_49_PENALTY=0.401426
PRIMER_LEFT_49_SEQUENCE=ACATGCTCCAACAACTCCAA
PRIMER_RIGHT_49_SEQUENCE=TACAAGAGTGGCGCATCAAA
PRIMER_LEFT_49=905,20
PRIMER_RIGHT_49=1307,20
PRIMER_LEFT_49_TM=59.139
PRIMER_RIGHT_49_TM=60.401
PRIMER_LEFT_49_GC_PERCENT=45.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=8.5000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=403
=
SEQUENCE_ID=MAGIC.329_3_14533107_14533606_sf-2_bur-0_5437_2514_TRUE
SEQUENCE_TARGET=950,599
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_5211102_5211361_can-0_po-0_12718_198400_TRUE
SEQUENCE_TARGET=950,359
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACATAATAGACANATATACTCTCTTTTCTNCTAAAACACNTNAAANTAATGGCTCTTGAAGACTAATGGTTACGTCACCATAGAGGAACANNNGAAGTGNATNGATGGCAGTCTGGTCTAGACCTGAGTCNTGTNGGNTACAAATCAAAGGTCCCACATCGNAAGTTTAGAAAAANANNTTCTAATATATAANGCAAGTNCANCTCCAACTAGTATAAGNCCTTGGNAAGAAGTNCAATAACAAATCNTTNCNGGNNNNNNNNNNNNNNNNNNNAGAGGACAATGTCATACTAAATGGAAGTTGGAATGGTCTTAGAAAAGCNATTAAATGGTATNAGAGCCTAGGTTGAAAAAATGGACCTANGGCTTAGGNTATGATCNNTGTNGGAGAATTTNTCAAACATGACANGATAAGAAGGTGCCTCAAGAATTTCACGAATAAGAAGGTGCCAAGTTAAGGGTGCNAAAGTTTCGTNGAAGAGGAGACCCANGCTCGTGGTCTTTGGTTTGAAGGGNAGATTGTTTGGTANAAAGTAAAGTTCTCANATCGGAAGTTTAGAAAAAGAGTTTCTNATATATAANCCAAGTCCAANTCCAACTNGTATGANGCCTNGGGAAGGAGNCCAATAACAAATCCTTGCANGCTTGAGCATGTAAGCCCNAAAANGACAATATCATACTNAATGGNAGTTGGAATGGGCTTNGAAAGCCCATTAAGTCATGGAGGAAGAAGAGGTGATGGAGCTGAGGTGAGGATGAAGAAANGGGGAANNGNAAATAAAGTCGANGAGAAANTTTGATATCAAATAGAGGATACCNTGGAAGACAATATGATAAGAGCAAGAACANAGATAACTAAACATAAAACAGAGANTGTAGAGCTTGAANATGGATCTNTCTNGNTNATAAGTGTNATTNTTGTTGCAGATGAAGATTTTGCTTTNTAAGATGTAAGAGAGAAATNTAAGCAGAACGATGAACATTAAATTCNCATGTTTCTCTTGCTCNCTCTGTTTGGTTAAAAANAGTTCAANTGTNTGGNTAGAGATNCANAGAAGCTAACTTTAGTCATCNGCAAAGATTATCNAAAAAACATCTTAANATAAAACATATGAATAGNCATGNGATTGTTTGCTGTTANTTANACCCAAAAAAAGATGCAATAGATACTAACTAATTAGCCGTTCCAAAAGAAGATAAACNTGAAAGTCATAAAGGTTCCAACACAAAACCAAAAAGAAAAACATAGGCAAATGCATCTCTAAATAGATAAAGACTAAACATATTTAGCCATCAAAGANNNNNNNNNNNNNNNNNNNNNNNNNNTCTCCCACATCNTTTTNAGCTCATCAGCTTGCACGGACTTCACCTTTTCTTCTATCCTNTTCCTCTTCTTCATNGGTACCAANCTCCAATTCTTCTTCCATGTCTCTTCATCAACCCCCNAATCCAATTCCTTAAACGCACCACCAACAAACGACCTCTCGAACCAATCACTTTCTTCATCAACTTAAGCTTNTTCCTCTTTGGCTTCNCAAGAGTAGCNACTCANTCTCAGTCTCCAACTCTTAATCTGAATTTTGATNGACAACANCAGTTTCTNGAGTCTTCTTACNAAAATCGTCTTCCTCCTCCTNCTCAGATGAAGAACNNGAGTTTTCTTCTTCGTCGCTANAAGACTCNGGCNGTGTAATTTTAACCTGTTTTCGTTGATTCTTCGAAGTTGATGCGACNACTTCCAACGGNNTCTTCGCCAGAGATGCGAANTCGATGNAGTAACCAAAGGCTGCNATTGCTTATTCTTCACCATNNGAATTATTGAAACNAGAGATAAATTTGAAANCTTNTANAATTTTAGGGTTTGTTATGAGAAGTTGNANNTGTANAATCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.581671
PRIMER_LEFT_0_PENALTY=0.519710
PRIMER_RIGHT_0_PENALTY=0.061961
PRIMER_LEFT_0_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_0_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_0=720,20
PRIMER_RIGHT_0=1693,20
PRIMER_LEFT_0_TM=60.520
PRIMER_RIGHT_0_TM=59.938
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.2000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=974
PRIMER_PAIR_1_PENALTY=0.637316
PRIMER_LEFT_1_PENALTY=0.575355
PRIMER_RIGHT_1_PENALTY=0.061961
PRIMER_LEFT_1_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_1_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_1=721,20
PRIMER_RIGHT_1=1693,20
PRIMER_LEFT_1_TM=59.425
PRIMER_RIGHT_1_TM=59.938
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=973
PRIMER_PAIR_2_PENALTY=1.045357
PRIMER_LEFT_2_PENALTY=0.519710
PRIMER_RIGHT_2_PENALTY=0.525647
PRIMER_LEFT_2_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_2_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_2=720,20
PRIMER_RIGHT_2=1710,20
PRIMER_LEFT_2_TM=60.520
PRIMER_RIGHT_2_TM=59.474
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=991
PRIMER_PAIR_3_PENALTY=1.064841
PRIMER_LEFT_3_PENALTY=0.519710
PRIMER_RIGHT_3_PENALTY=0.545130
PRIMER_LEFT_3_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_3_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_3=720,20
PRIMER_RIGHT_3=1711,20
PRIMER_LEFT_3_TM=60.520
PRIMER_RIGHT_3_TM=60.545
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=992
PRIMER_PAIR_4_PENALTY=1.101002
PRIMER_LEFT_4_PENALTY=0.575355
PRIMER_RIGHT_4_PENALTY=0.525647
PRIMER_LEFT_4_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_4_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_4=721,20
PRIMER_RIGHT_4=1710,20
PRIMER_LEFT_4_TM=59.425
PRIMER_RIGHT_4_TM=59.474
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=990
PRIMER_PAIR_5_PENALTY=1.120486
PRIMER_LEFT_5_PENALTY=0.575355
PRIMER_RIGHT_5_PENALTY=0.545130
PRIMER_LEFT_5_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_5_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_5=721,20
PRIMER_RIGHT_5=1711,20
PRIMER_LEFT_5_TM=59.425
PRIMER_RIGHT_5_TM=60.545
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=991
PRIMER_PAIR_6_PENALTY=1.209391
PRIMER_LEFT_6_PENALTY=0.519710
PRIMER_RIGHT_6_PENALTY=0.689681
PRIMER_LEFT_6_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_6_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_6=720,20
PRIMER_RIGHT_6=1713,20
PRIMER_LEFT_6_TM=60.520
PRIMER_RIGHT_6_TM=60.690
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=994
PRIMER_PAIR_7_PENALTY=1.209391
PRIMER_LEFT_7_PENALTY=0.519710
PRIMER_RIGHT_7_PENALTY=0.689681
PRIMER_LEFT_7_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_7_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_7=720,20
PRIMER_RIGHT_7=1717,20
PRIMER_LEFT_7_TM=60.520
PRIMER_RIGHT_7_TM=60.690
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=998
PRIMER_PAIR_8_PENALTY=1.265036
PRIMER_LEFT_8_PENALTY=0.575355
PRIMER_RIGHT_8_PENALTY=0.689681
PRIMER_LEFT_8_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_8_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_8=721,20
PRIMER_RIGHT_8=1713,20
PRIMER_LEFT_8_TM=59.425
PRIMER_RIGHT_8_TM=60.690
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=993
PRIMER_PAIR_9_PENALTY=1.265036
PRIMER_LEFT_9_PENALTY=0.575355
PRIMER_RIGHT_9_PENALTY=0.689681
PRIMER_LEFT_9_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_9_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_9=721,20
PRIMER_RIGHT_9=1717,20
PRIMER_LEFT_9_TM=59.425
PRIMER_RIGHT_9_TM=60.690
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=997
PRIMER_PAIR_10_PENALTY=1.277948
PRIMER_LEFT_10_PENALTY=1.215987
PRIMER_RIGHT_10_PENALTY=0.061961
PRIMER_LEFT_10_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_10_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_10=717,20
PRIMER_RIGHT_10=1693,20
PRIMER_LEFT_10_TM=61.216
PRIMER_RIGHT_10_TM=59.938
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=977
PRIMER_PAIR_11_PENALTY=1.302355
PRIMER_LEFT_11_PENALTY=1.240394
PRIMER_RIGHT_11_PENALTY=0.061961
PRIMER_LEFT_11_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_11_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_11=718,21
PRIMER_RIGHT_11=1693,20
PRIMER_LEFT_11_TM=59.760
PRIMER_RIGHT_11_TM=59.938
PRIMER_LEFT_11_GC_PERCENT=47.619
PRIMER_RIGHT_11_GC_PERCENT=45.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=976
PRIMER_PAIR_12_PENALTY=1.315672
PRIMER_LEFT_12_PENALTY=1.253711
PRIMER_RIGHT_12_PENALTY=0.061961
PRIMER_LEFT_12_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_12_SEQUENCE=GGTGCGTTTAAGGAATTGGA
PRIMER_LEFT_12=719,20
PRIMER_RIGHT_12=1693,20
PRIMER_LEFT_12_TM=58.746
PRIMER_RIGHT_12_TM=59.938
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=975
PRIMER_PAIR_13_PENALTY=1.332731
PRIMER_LEFT_13_PENALTY=0.519710
PRIMER_RIGHT_13_PENALTY=0.813021
PRIMER_LEFT_13_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_13_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_13=720,20
PRIMER_RIGHT_13=1716,20
PRIMER_LEFT_13_TM=60.520
PRIMER_RIGHT_13_TM=59.187
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=997
PRIMER_PAIR_14_PENALTY=1.375804
PRIMER_LEFT_14_PENALTY=0.519710
PRIMER_RIGHT_14_PENALTY=0.856094
PRIMER_LEFT_14_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_14_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_14=720,20
PRIMER_RIGHT_14=1694,20
PRIMER_LEFT_14_TM=60.520
PRIMER_RIGHT_14_TM=60.856
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=975
PRIMER_PAIR_15_PENALTY=1.388376
PRIMER_LEFT_15_PENALTY=0.575355
PRIMER_RIGHT_15_PENALTY=0.813021
PRIMER_LEFT_15_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_15_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_15=721,20
PRIMER_RIGHT_15=1716,20
PRIMER_LEFT_15_TM=59.425
PRIMER_RIGHT_15_TM=59.187
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=996
PRIMER_PAIR_16_PENALTY=1.431449
PRIMER_LEFT_16_PENALTY=0.575355
PRIMER_RIGHT_16_PENALTY=0.856094
PRIMER_LEFT_16_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_16_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_16=721,20
PRIMER_RIGHT_16=1694,20
PRIMER_LEFT_16_TM=59.425
PRIMER_RIGHT_16_TM=60.856
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=974
PRIMER_PAIR_17_PENALTY=1.434568
PRIMER_LEFT_17_PENALTY=0.575355
PRIMER_RIGHT_17_PENALTY=0.859212
PRIMER_LEFT_17_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_17_SEQUENCE=GATTGGTTCGAGAGGTCGTT
PRIMER_LEFT_17=721,20
PRIMER_RIGHT_17=1720,20
PRIMER_LEFT_17_TM=59.425
PRIMER_RIGHT_17_TM=59.141
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=1000
PRIMER_PAIR_18_PENALTY=1.443460
PRIMER_LEFT_18_PENALTY=0.519710
PRIMER_RIGHT_18_PENALTY=0.923750
PRIMER_LEFT_18_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_18_SEQUENCE=GTTGGTGGTGCGTTTAAGGA
PRIMER_LEFT_18=720,20
PRIMER_RIGHT_18=1699,20
PRIMER_LEFT_18_TM=60.520
PRIMER_RIGHT_18_TM=60.924
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=980
PRIMER_PAIR_19_PENALTY=1.499105
PRIMER_LEFT_19_PENALTY=0.575355
PRIMER_RIGHT_19_PENALTY=0.923750
PRIMER_LEFT_19_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_19_SEQUENCE=GTTGGTGGTGCGTTTAAGGA
PRIMER_LEFT_19=721,20
PRIMER_RIGHT_19=1699,20
PRIMER_LEFT_19_TM=59.425
PRIMER_RIGHT_19_TM=60.924
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=979
PRIMER_PAIR_20_PENALTY=1.601299
PRIMER_LEFT_20_PENALTY=0.519710
PRIMER_RIGHT_20_PENALTY=1.081589
PRIMER_LEFT_20_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_20_SEQUENCE=GGGTTGATGAAGAGACATGGA
PRIMER_LEFT_20=720,20
PRIMER_RIGHT_20=1668,21
PRIMER_LEFT_20_TM=60.520
PRIMER_RIGHT_20_TM=59.918
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.1000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=949
PRIMER_PAIR_21_PENALTY=1.656944
PRIMER_LEFT_21_PENALTY=0.575355
PRIMER_RIGHT_21_PENALTY=1.081589
PRIMER_LEFT_21_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_21_SEQUENCE=GGGTTGATGAAGAGACATGGA
PRIMER_LEFT_21=721,20
PRIMER_RIGHT_21=1668,21
PRIMER_LEFT_21_TM=59.425
PRIMER_RIGHT_21_TM=59.918
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=948
PRIMER_PAIR_22_PENALTY=1.723611
PRIMER_LEFT_22_PENALTY=0.519710
PRIMER_RIGHT_22_PENALTY=1.203901
PRIMER_LEFT_22_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_22_SEQUENCE=TTCGAGAGGTCGTTTGTTGG
PRIMER_LEFT_22=720,20
PRIMER_RIGHT_22=1714,20
PRIMER_LEFT_22_TM=60.520
PRIMER_RIGHT_22_TM=61.204
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=995
PRIMER_PAIR_23_PENALTY=1.723611
PRIMER_LEFT_23_PENALTY=0.519710
PRIMER_RIGHT_23_PENALTY=1.203901
PRIMER_LEFT_23_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_23_SEQUENCE=TTGGTTCGAGAGGTCGTTTG
PRIMER_LEFT_23=720,20
PRIMER_RIGHT_23=1718,20
PRIMER_LEFT_23_TM=60.520
PRIMER_RIGHT_23_TM=61.204
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=999
PRIMER_PAIR_24_PENALTY=1.741634
PRIMER_LEFT_24_PENALTY=1.215987
PRIMER_RIGHT_24_PENALTY=0.525647
PRIMER_LEFT_24_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_24_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_24=717,20
PRIMER_RIGHT_24=1710,20
PRIMER_LEFT_24_TM=61.216
PRIMER_RIGHT_24_TM=59.474
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.3000
PRIMER_RIGHT_24_END_STABILITY=7.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=994
PRIMER_PAIR_25_PENALTY=1.761118
PRIMER_LEFT_25_PENALTY=1.215987
PRIMER_RIGHT_25_PENALTY=0.545130
PRIMER_LEFT_25_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_25_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_25=717,20
PRIMER_RIGHT_25=1711,20
PRIMER_LEFT_25_TM=61.216
PRIMER_RIGHT_25_TM=60.545
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.3000
PRIMER_RIGHT_25_END_STABILITY=9.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=995
PRIMER_PAIR_26_PENALTY=1.766041
PRIMER_LEFT_26_PENALTY=1.240394
PRIMER_RIGHT_26_PENALTY=0.525647
PRIMER_LEFT_26_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_26_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_26=718,21
PRIMER_RIGHT_26=1710,20
PRIMER_LEFT_26_TM=59.760
PRIMER_RIGHT_26_TM=59.474
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=7.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=993
PRIMER_PAIR_27_PENALTY=1.779256
PRIMER_LEFT_27_PENALTY=0.575355
PRIMER_RIGHT_27_PENALTY=1.203901
PRIMER_LEFT_27_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_27_SEQUENCE=TTCGAGAGGTCGTTTGTTGG
PRIMER_LEFT_27=721,20
PRIMER_RIGHT_27=1714,20
PRIMER_LEFT_27_TM=59.425
PRIMER_RIGHT_27_TM=61.204
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=994
PRIMER_PAIR_28_PENALTY=1.779256
PRIMER_LEFT_28_PENALTY=0.575355
PRIMER_RIGHT_28_PENALTY=1.203901
PRIMER_LEFT_28_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_28_SEQUENCE=TTGGTTCGAGAGGTCGTTTG
PRIMER_LEFT_28=721,20
PRIMER_RIGHT_28=1718,20
PRIMER_LEFT_28_TM=59.425
PRIMER_RIGHT_28_TM=61.204
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=998
PRIMER_PAIR_29_PENALTY=1.779359
PRIMER_LEFT_29_PENALTY=1.253711
PRIMER_RIGHT_29_PENALTY=0.525647
PRIMER_LEFT_29_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_29_SEQUENCE=AGAGGTCGTTTGTTGGTGGT
PRIMER_LEFT_29=719,20
PRIMER_RIGHT_29=1710,20
PRIMER_LEFT_29_TM=58.746
PRIMER_RIGHT_29_TM=59.474
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=7.6000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=992
PRIMER_PAIR_30_PENALTY=1.785525
PRIMER_LEFT_30_PENALTY=1.240394
PRIMER_RIGHT_30_PENALTY=0.545130
PRIMER_LEFT_30_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_30_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_30=718,21
PRIMER_RIGHT_30=1711,20
PRIMER_LEFT_30_TM=59.760
PRIMER_RIGHT_30_TM=60.545
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=9.4000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=994
PRIMER_PAIR_31_PENALTY=1.798842
PRIMER_LEFT_31_PENALTY=1.253711
PRIMER_RIGHT_31_PENALTY=0.545130
PRIMER_LEFT_31_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_31_SEQUENCE=GAGAGGTCGTTTGTTGGTGG
PRIMER_LEFT_31=719,20
PRIMER_RIGHT_31=1711,20
PRIMER_LEFT_31_TM=58.746
PRIMER_RIGHT_31_TM=60.545
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=993
PRIMER_PAIR_32_PENALTY=1.873862
PRIMER_LEFT_32_PENALTY=0.519710
PRIMER_RIGHT_32_PENALTY=1.354152
PRIMER_LEFT_32_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_32_SEQUENCE=ATTGGTTCGAGAGGTCGTTT
PRIMER_LEFT_32=720,20
PRIMER_RIGHT_32=1719,20
PRIMER_LEFT_32_TM=60.520
PRIMER_RIGHT_32_TM=58.646
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=8.2000
PRIMER_RIGHT_32_END_STABILITY=8.7000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=1000
PRIMER_PAIR_33_PENALTY=1.905668
PRIMER_LEFT_33_PENALTY=1.215987
PRIMER_RIGHT_33_PENALTY=0.689681
PRIMER_LEFT_33_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_33_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_33=717,20
PRIMER_RIGHT_33=1713,20
PRIMER_LEFT_33_TM=61.216
PRIMER_RIGHT_33_TM=60.690
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=7.3000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=997
PRIMER_PAIR_34_PENALTY=1.929508
PRIMER_LEFT_34_PENALTY=0.575355
PRIMER_RIGHT_34_PENALTY=1.354152
PRIMER_LEFT_34_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_34_SEQUENCE=ATTGGTTCGAGAGGTCGTTT
PRIMER_LEFT_34=721,20
PRIMER_RIGHT_34=1719,20
PRIMER_LEFT_34_TM=59.425
PRIMER_RIGHT_34_TM=58.646
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=8.7000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=999
PRIMER_PAIR_35_PENALTY=1.929650
PRIMER_LEFT_35_PENALTY=0.519710
PRIMER_RIGHT_35_PENALTY=1.409940
PRIMER_LEFT_35_SEQUENCE=CGTGGTCTTTGGTTTGAAGG
PRIMER_RIGHT_35_SEQUENCE=GAGGTCGTTTGTTGGTGGTG
PRIMER_LEFT_35=720,20
PRIMER_RIGHT_35=1709,20
PRIMER_LEFT_35_TM=60.520
PRIMER_RIGHT_35_TM=61.410
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=2.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=990
PRIMER_PAIR_36_PENALTY=1.930075
PRIMER_LEFT_36_PENALTY=1.240394
PRIMER_RIGHT_36_PENALTY=0.689681
PRIMER_LEFT_36_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_36_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_36=718,21
PRIMER_RIGHT_36=1713,20
PRIMER_LEFT_36_TM=59.760
PRIMER_RIGHT_36_TM=60.690
PRIMER_LEFT_36_GC_PERCENT=47.619
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=996
PRIMER_PAIR_37_PENALTY=1.930075
PRIMER_LEFT_37_PENALTY=1.240394
PRIMER_RIGHT_37_PENALTY=0.689681
PRIMER_LEFT_37_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_37_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_37=718,21
PRIMER_RIGHT_37=1717,20
PRIMER_LEFT_37_TM=59.760
PRIMER_RIGHT_37_TM=60.690
PRIMER_LEFT_37_GC_PERCENT=47.619
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=1000
PRIMER_PAIR_38_PENALTY=1.943393
PRIMER_LEFT_38_PENALTY=1.253711
PRIMER_RIGHT_38_PENALTY=0.689681
PRIMER_LEFT_38_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_38_SEQUENCE=TCGAGAGGTCGTTTGTTGGT
PRIMER_LEFT_38=719,20
PRIMER_RIGHT_38=1713,20
PRIMER_LEFT_38_TM=58.746
PRIMER_RIGHT_38_TM=60.690
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=995
PRIMER_PAIR_39_PENALTY=1.943393
PRIMER_LEFT_39_PENALTY=1.253711
PRIMER_RIGHT_39_PENALTY=0.689681
PRIMER_LEFT_39_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_39_SEQUENCE=TGGTTCGAGAGGTCGTTTGT
PRIMER_LEFT_39=719,20
PRIMER_RIGHT_39=1717,20
PRIMER_LEFT_39_TM=58.746
PRIMER_RIGHT_39_TM=60.690
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=999
PRIMER_PAIR_40_PENALTY=1.984107
PRIMER_LEFT_40_PENALTY=0.902519
PRIMER_RIGHT_40_PENALTY=1.081589
PRIMER_LEFT_40_SEQUENCE=AAGGTGCCAAGTTAAGGGTG
PRIMER_RIGHT_40_SEQUENCE=GGGTTGATGAAGAGACATGGA
PRIMER_LEFT_40=669,20
PRIMER_RIGHT_40=1668,21
PRIMER_LEFT_40_TM=59.097
PRIMER_RIGHT_40_TM=59.918
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=9.4000
PRIMER_RIGHT_40_END_STABILITY=8.1000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=1000
PRIMER_PAIR_41_PENALTY=1.985296
PRIMER_LEFT_41_PENALTY=0.575355
PRIMER_RIGHT_41_PENALTY=1.409940
PRIMER_LEFT_41_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_41_SEQUENCE=GAGGTCGTTTGTTGGTGGTG
PRIMER_LEFT_41=721,20
PRIMER_RIGHT_41=1709,20
PRIMER_LEFT_41_TM=59.425
PRIMER_RIGHT_41_TM=61.410
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=3.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=2.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=989
PRIMER_PAIR_42_PENALTY=2.029008
PRIMER_LEFT_42_PENALTY=1.215987
PRIMER_RIGHT_42_PENALTY=0.813021
PRIMER_LEFT_42_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_42_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_42=717,20
PRIMER_RIGHT_42=1716,20
PRIMER_LEFT_42_TM=61.216
PRIMER_RIGHT_42_TM=59.187
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=1000
PRIMER_PAIR_43_PENALTY=2.053415
PRIMER_LEFT_43_PENALTY=1.240394
PRIMER_RIGHT_43_PENALTY=0.813021
PRIMER_LEFT_43_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_43_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_43=718,21
PRIMER_RIGHT_43=1716,20
PRIMER_LEFT_43_TM=59.760
PRIMER_RIGHT_43_TM=59.187
PRIMER_LEFT_43_GC_PERCENT=47.619
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.0000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=999
PRIMER_PAIR_44_PENALTY=2.065669
PRIMER_LEFT_44_PENALTY=0.575355
PRIMER_RIGHT_44_PENALTY=1.490313
PRIMER_LEFT_44_SEQUENCE=GTGGTCTTTGGTTTGAAGGG
PRIMER_RIGHT_44_SEQUENCE=GATTGGTTCGAGAGGTCGTTT
PRIMER_LEFT_44=721,20
PRIMER_RIGHT_44=1720,21
PRIMER_LEFT_44_TM=59.425
PRIMER_RIGHT_44_TM=60.490
PRIMER_LEFT_44_GC_PERCENT=50.000
PRIMER_RIGHT_44_GC_PERCENT=47.619
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=9.7000
PRIMER_RIGHT_44_END_STABILITY=8.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=1000
PRIMER_PAIR_45_PENALTY=2.066732
PRIMER_LEFT_45_PENALTY=1.253711
PRIMER_RIGHT_45_PENALTY=0.813021
PRIMER_LEFT_45_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_45_SEQUENCE=GGTTCGAGAGGTCGTTTGTT
PRIMER_LEFT_45=719,20
PRIMER_RIGHT_45=1716,20
PRIMER_LEFT_45_TM=58.746
PRIMER_RIGHT_45_TM=59.187
PRIMER_LEFT_45_GC_PERCENT=45.000
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=998
PRIMER_PAIR_46_PENALTY=2.072081
PRIMER_LEFT_46_PENALTY=1.215987
PRIMER_RIGHT_46_PENALTY=0.856094
PRIMER_LEFT_46_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_46_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_46=717,20
PRIMER_RIGHT_46=1694,20
PRIMER_LEFT_46_TM=61.216
PRIMER_RIGHT_46_TM=60.856
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.3000
PRIMER_RIGHT_46_END_STABILITY=8.4000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=978
PRIMER_PAIR_47_PENALTY=2.096488
PRIMER_LEFT_47_PENALTY=1.240394
PRIMER_RIGHT_47_PENALTY=0.856094
PRIMER_LEFT_47_SEQUENCE=CTCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_47_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_47=718,21
PRIMER_RIGHT_47=1694,20
PRIMER_LEFT_47_TM=59.760
PRIMER_RIGHT_47_TM=60.856
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.0000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=977
PRIMER_PAIR_48_PENALTY=2.109805
PRIMER_LEFT_48_PENALTY=1.253711
PRIMER_RIGHT_48_PENALTY=0.856094
PRIMER_LEFT_48_SEQUENCE=TCGTGGTCTTTGGTTTGAAG
PRIMER_RIGHT_48_SEQUENCE=TGGTGCGTTTAAGGAATTGG
PRIMER_LEFT_48=719,20
PRIMER_RIGHT_48=1694,20
PRIMER_LEFT_48_TM=58.746
PRIMER_RIGHT_48_TM=60.856
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=8.4000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=976
PRIMER_PAIR_49_PENALTY=2.139737
PRIMER_LEFT_49_PENALTY=1.215987
PRIMER_RIGHT_49_PENALTY=0.923750
PRIMER_LEFT_49_SEQUENCE=GCTCGTGGTCTTTGGTTTGA
PRIMER_RIGHT_49_SEQUENCE=GTTGGTGGTGCGTTTAAGGA
PRIMER_LEFT_49=717,20
PRIMER_RIGHT_49=1699,20
PRIMER_LEFT_49_TM=61.216
PRIMER_RIGHT_49_TM=60.924
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.3000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=983
=
SEQUENCE_ID=MAGIC.105_4_16696549_16696935_no-0_tsu-0_22449_37576_TRUE
SEQUENCE_TARGET=950,486
SEQUENCE_TEMPLATE=TCTCAAGAAGTAAATTCTGATTAGGGTTTTCATTTTCCCTTTTTGTTTTTTGGTTCTTTTTCCATTCAATGGAGNAAAAAAAGNAACCCAGCTGGATTTGATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAAGCAATGAAAAAGTAAANAAAAAGAAAGGAAGCATTATATATTAATGGGTTTGTTTTGATGTAGGAAGATGGTGAAAAGAGGTGGTAGTAGAGGCCGTAGACACGCGTATTGGAGGAAGCTGTAAGTATTATTGNGTTGCTCAACAATCAATNTAGTACAGTGACATGAGAGCACAATAGTAAGTANNNNNTCCACATNAAAAAAATAGAAATTGAAAGAGAATCAGAGTTTGGCCTGCCATAGGATTTGAGTTCTCGCACTTTCCTAAACAGAAACATGTTTCATTGAACACTTGGGCCTTTCTTCTTCCTTCATTAAGGATTCTCGATCTCTGTTTCGACAGATCTGTGCTACCTAGGTTTTCTAGATACTCCCTAATACCTATAAATAAACAATAAGTATAACCTTATTATGTAATTAGATCGTACTACTATCAATAAACAAGATTGTAGAAAATAGTGAAAAAGGCAACGCATAAAGATTAATTAAATCTTATTTAAGTTTCATTTGGGACAATTTAANATNATTGTCTTGTTCATAGGATTTATTTCATCTATTAGTATTACAAACTTTTTTACNNAAACATTATTANCTCTANGTATAGAATGATTTAAACTTTTAACGTAAACACAAATAAACAGTCAGTCCTATGTGCTATGGATGAGGTGATTAGCATTGTAGGGTGTTTCGTGTCATATAGCTCTACTAGTAGAGATTTTGTGTGTCAGTGTGTGTTTATTTACGGCTTTGAGTCTTCAAGACCCAATTAGCCTTAAGACCTTAGTGATTTTAGACTCTTTGTTGAAAAGCTAGAAATTNCAAACCCAAACCGCCTAATGTTGCTATAGGTTTTGATTTCCGAAGTGGGTAATTAATTATGTGGATTACGTATAAACACATGGCCCGGCCCNTTTNANCCCCCAAGTGCTCGGCCCAAATTGAAAGTGGGACTCACTGGATTGCCAACTAATTTTATTTAACACGACGTGAAGTTTGGAACTAATACGTCANGTATTAAAAGTACATAAACATCATCATTACTCAATTTGCTAATGGACGAATAGAGACGGAATCAACAAAGTAACANACGACTTCAATATTTTTTTTATATGTTACTATGATTCGACAAAGGANTTTTTTTTTGTTATGAACTGAAGATTATTGGATTTTTAATCACAAGATGTCAATATCAAAATTCAATACCATGTCTCGATTAGGGATATCAATATCATGATATTTTAACAACTTTAACTATGTAATTTAATTCGATTATCGTTTTTGTTTTAACCTTCGTTTGATTTGTAGATGGTAGAGGTATTTAGTTGTTGAACTCTTCTTTATACACATGTTGTTTGTTTTGCTTCTTAAAAGGATAATAATTTNNAAACTTATTACATGTGCACCACTGNTCGCATTCGTAGTTTTCCATATTTTATGCGAAAGAAATGAGGATATCTTGGGAAGATACAAATTATTGGGATTGTTTTGCAATAACTCCAAAATGTTATGTTTAAGAGTTTTTTGCTTACGTATTTCATATCAAGCCACGTAGATGCATTTGTAATCTCATAAAACATTTCGATTANNATTNACTTACTGCACCATATTAAAAAACAAATTTCGATATCGAGATTGTAAATTGGGCAATTAGGAATTAGGAATTNAGGTAGGTAAAGTTTATAAGCAGATGAGGAAATAATAGCAAGGAAGAGAAAGAAAGTGGATGCGTCGTGGATTCCATATTTATGTGCTTTACTTTTCCTTTTGGTAGGCGAAAATTTTGACATTTTCCAACAAACATCCTCATGTATTTCACATATTGGCCTGACCAACTATTATGCATATTTTATACATTTGATGGATTAGGCTATAACATGGTTTTTGGAGTTTACTTTTATACGTTCGATCAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.889703
PRIMER_LEFT_0_PENALTY=0.029253
PRIMER_RIGHT_0_PENALTY=1.860449
PRIMER_LEFT_0_SEQUENCE=CATTGAACACTTGGGCCTTT
PRIMER_RIGHT_0_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_0=758,20
PRIMER_RIGHT_0=1549,21
PRIMER_LEFT_0_TM=59.971
PRIMER_RIGHT_0_TM=59.140
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=7.9000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=792
PRIMER_PAIR_1_PENALTY=1.889703
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=1.860449
PRIMER_LEFT_1_SEQUENCE=CATTGAACACTTGGGCCTTT
PRIMER_RIGHT_1_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_1=758,20
PRIMER_RIGHT_1=1550,21
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.140
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=793
PRIMER_PAIR_2_PENALTY=2.039870
PRIMER_LEFT_2_PENALTY=0.179421
PRIMER_RIGHT_2_PENALTY=1.860449
PRIMER_LEFT_2_SEQUENCE=CTTGGGCCTTTCTTCTTCCT
PRIMER_RIGHT_2_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_2=767,20
PRIMER_RIGHT_2=1549,21
PRIMER_LEFT_2_TM=59.821
PRIMER_RIGHT_2_TM=59.140
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=783
PRIMER_PAIR_3_PENALTY=2.039870
PRIMER_LEFT_3_PENALTY=0.179421
PRIMER_RIGHT_3_PENALTY=1.860449
PRIMER_LEFT_3_SEQUENCE=CTTGGGCCTTTCTTCTTCCT
PRIMER_RIGHT_3_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_3=767,20
PRIMER_RIGHT_3=1550,21
PRIMER_LEFT_3_TM=59.821
PRIMER_RIGHT_3_TM=59.140
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=784
PRIMER_PAIR_4_PENALTY=2.040044
PRIMER_LEFT_4_PENALTY=0.179594
PRIMER_RIGHT_4_PENALTY=1.860449
PRIMER_LEFT_4_SEQUENCE=TTGGGCCTTTCTTCTTCCTT
PRIMER_RIGHT_4_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_4=768,20
PRIMER_RIGHT_4=1549,21
PRIMER_LEFT_4_TM=60.180
PRIMER_RIGHT_4_TM=59.140
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=782
PRIMER_PAIR_5_PENALTY=2.040044
PRIMER_LEFT_5_PENALTY=0.179594
PRIMER_RIGHT_5_PENALTY=1.860449
PRIMER_LEFT_5_SEQUENCE=TTGGGCCTTTCTTCTTCCTT
PRIMER_RIGHT_5_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_5=768,20
PRIMER_RIGHT_5=1550,21
PRIMER_LEFT_5_TM=60.180
PRIMER_RIGHT_5_TM=59.140
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=2.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=783
PRIMER_PAIR_6_PENALTY=2.149254
PRIMER_LEFT_6_PENALTY=0.288804
PRIMER_RIGHT_6_PENALTY=1.860449
PRIMER_LEFT_6_SEQUENCE=TGAACACTTGGGCCTTTCTT
PRIMER_RIGHT_6_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_6=761,20
PRIMER_RIGHT_6=1549,21
PRIMER_LEFT_6_TM=59.711
PRIMER_RIGHT_6_TM=59.140
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=789
PRIMER_PAIR_7_PENALTY=2.149254
PRIMER_LEFT_7_PENALTY=0.288804
PRIMER_RIGHT_7_PENALTY=1.860449
PRIMER_LEFT_7_SEQUENCE=TGAACACTTGGGCCTTTCTT
PRIMER_RIGHT_7_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_7=761,20
PRIMER_RIGHT_7=1550,21
PRIMER_LEFT_7_TM=59.711
PRIMER_RIGHT_7_TM=59.140
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=47.619
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=790
PRIMER_PAIR_8_PENALTY=2.149254
PRIMER_LEFT_8_PENALTY=0.288804
PRIMER_RIGHT_8_PENALTY=1.860449
PRIMER_LEFT_8_SEQUENCE=TTGAACACTTGGGCCTTTCT
PRIMER_RIGHT_8_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_8=760,20
PRIMER_RIGHT_8=1549,21
PRIMER_LEFT_8_TM=59.711
PRIMER_RIGHT_8_TM=59.140
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=790
PRIMER_PAIR_9_PENALTY=2.149254
PRIMER_LEFT_9_PENALTY=0.288804
PRIMER_RIGHT_9_PENALTY=1.860449
PRIMER_LEFT_9_SEQUENCE=TTGAACACTTGGGCCTTTCT
PRIMER_RIGHT_9_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_9=760,20
PRIMER_RIGHT_9=1550,21
PRIMER_LEFT_9_TM=59.711
PRIMER_RIGHT_9_TM=59.140
PRIMER_LEFT_9_GC_PERCENT=45.000
PRIMER_RIGHT_9_GC_PERCENT=47.619
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=791
PRIMER_PAIR_10_PENALTY=2.169867
PRIMER_LEFT_10_PENALTY=0.309418
PRIMER_RIGHT_10_PENALTY=1.860449
PRIMER_LEFT_10_SEQUENCE=ACTTGGGCCTTTCTTCTTCC
PRIMER_RIGHT_10_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_10=766,20
PRIMER_RIGHT_10=1549,21
PRIMER_LEFT_10_TM=59.691
PRIMER_RIGHT_10_TM=59.140
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=47.619
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=784
PRIMER_PAIR_11_PENALTY=2.169867
PRIMER_LEFT_11_PENALTY=0.309418
PRIMER_RIGHT_11_PENALTY=1.860449
PRIMER_LEFT_11_SEQUENCE=ACTTGGGCCTTTCTTCTTCC
PRIMER_RIGHT_11_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_11=766,20
PRIMER_RIGHT_11=1550,21
PRIMER_LEFT_11_TM=59.691
PRIMER_RIGHT_11_TM=59.140
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=2.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=785
PRIMER_PAIR_12_PENALTY=2.355622
PRIMER_LEFT_12_PENALTY=0.495173
PRIMER_RIGHT_12_PENALTY=1.860449
PRIMER_LEFT_12_SEQUENCE=TCATTGAACACTTGGGCCTT
PRIMER_RIGHT_12_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_12=757,20
PRIMER_RIGHT_12=1549,21
PRIMER_LEFT_12_TM=60.495
PRIMER_RIGHT_12_TM=59.140
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=793
PRIMER_PAIR_13_PENALTY=2.355622
PRIMER_LEFT_13_PENALTY=0.495173
PRIMER_RIGHT_13_PENALTY=1.860449
PRIMER_LEFT_13_SEQUENCE=TCATTGAACACTTGGGCCTT
PRIMER_RIGHT_13_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_13=757,20
PRIMER_RIGHT_13=1550,21
PRIMER_LEFT_13_TM=60.495
PRIMER_RIGHT_13_TM=59.140
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=794
PRIMER_PAIR_14_PENALTY=2.370285
PRIMER_LEFT_14_PENALTY=0.029253
PRIMER_RIGHT_14_PENALTY=2.341032
PRIMER_LEFT_14_SEQUENCE=CATTGAACACTTGGGCCTTT
PRIMER_RIGHT_14_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_14=758,20
PRIMER_RIGHT_14=1547,21
PRIMER_LEFT_14_TM=59.971
PRIMER_RIGHT_14_TM=58.659
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=790
PRIMER_PAIR_15_PENALTY=2.480466
PRIMER_LEFT_15_PENALTY=0.029253
PRIMER_RIGHT_15_PENALTY=2.451213
PRIMER_LEFT_15_SEQUENCE=CATTGAACACTTGGGCCTTT
PRIMER_RIGHT_15_SEQUENCE=TTGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_15=758,20
PRIMER_RIGHT_15=1551,22
PRIMER_LEFT_15_TM=59.971
PRIMER_RIGHT_15_TM=60.451
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=45.455
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=794
PRIMER_PAIR_16_PENALTY=2.494043
PRIMER_LEFT_16_PENALTY=0.633593
PRIMER_RIGHT_16_PENALTY=1.860449
PRIMER_LEFT_16_SEQUENCE=CGTAGACACGCGTATTGGAG
PRIMER_RIGHT_16_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_16=570,20
PRIMER_RIGHT_16=1549,21
PRIMER_LEFT_16_TM=59.366
PRIMER_RIGHT_16_TM=59.140
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=980
PRIMER_PAIR_17_PENALTY=2.494043
PRIMER_LEFT_17_PENALTY=0.633593
PRIMER_RIGHT_17_PENALTY=1.860449
PRIMER_LEFT_17_SEQUENCE=CGTAGACACGCGTATTGGAG
PRIMER_RIGHT_17_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_17=570,20
PRIMER_RIGHT_17=1550,21
PRIMER_LEFT_17_TM=59.366
PRIMER_RIGHT_17_TM=59.140
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=981
PRIMER_PAIR_18_PENALTY=2.520453
PRIMER_LEFT_18_PENALTY=0.179421
PRIMER_RIGHT_18_PENALTY=2.341032
PRIMER_LEFT_18_SEQUENCE=CTTGGGCCTTTCTTCTTCCT
PRIMER_RIGHT_18_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_18=767,20
PRIMER_RIGHT_18=1547,21
PRIMER_LEFT_18_TM=59.821
PRIMER_RIGHT_18_TM=58.659
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=781
PRIMER_PAIR_19_PENALTY=2.520627
PRIMER_LEFT_19_PENALTY=0.179594
PRIMER_RIGHT_19_PENALTY=2.341032
PRIMER_LEFT_19_SEQUENCE=TTGGGCCTTTCTTCTTCCTT
PRIMER_RIGHT_19_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_19=768,20
PRIMER_RIGHT_19=1547,21
PRIMER_LEFT_19_TM=60.180
PRIMER_RIGHT_19_TM=58.659
PRIMER_LEFT_19_GC_PERCENT=45.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=780
PRIMER_PAIR_20_PENALTY=2.543712
PRIMER_LEFT_20_PENALTY=0.683262
PRIMER_RIGHT_20_PENALTY=1.860449
PRIMER_LEFT_20_SEQUENCE=GGGCCTTTCTTCTTCCTTCA
PRIMER_RIGHT_20_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_20=770,20
PRIMER_RIGHT_20=1549,21
PRIMER_LEFT_20_TM=60.683
PRIMER_RIGHT_20_TM=59.140
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=2.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=780
PRIMER_PAIR_21_PENALTY=2.543712
PRIMER_LEFT_21_PENALTY=0.683262
PRIMER_RIGHT_21_PENALTY=1.860449
PRIMER_LEFT_21_SEQUENCE=GGGCCTTTCTTCTTCCTTCA
PRIMER_RIGHT_21_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_21=770,20
PRIMER_RIGHT_21=1550,21
PRIMER_LEFT_21_TM=60.683
PRIMER_RIGHT_21_TM=59.140
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.0000
PRIMER_RIGHT_21_END_STABILITY=9.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=781
PRIMER_PAIR_22_PENALTY=2.543712
PRIMER_LEFT_22_PENALTY=0.683262
PRIMER_RIGHT_22_PENALTY=1.860449
PRIMER_LEFT_22_SEQUENCE=TGGGCCTTTCTTCTTCCTTC
PRIMER_RIGHT_22_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_22=769,20
PRIMER_RIGHT_22=1549,21
PRIMER_LEFT_22_TM=60.683
PRIMER_RIGHT_22_TM=59.140
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=47.619
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=781
PRIMER_PAIR_23_PENALTY=2.543712
PRIMER_LEFT_23_PENALTY=0.683262
PRIMER_RIGHT_23_PENALTY=1.860449
PRIMER_LEFT_23_SEQUENCE=TGGGCCTTTCTTCTTCCTTC
PRIMER_RIGHT_23_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_23=769,20
PRIMER_RIGHT_23=1550,21
PRIMER_LEFT_23_TM=60.683
PRIMER_RIGHT_23_TM=59.140
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=9.6000
PRIMER_PAIR_23_COMPL_ANY=2.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=782
PRIMER_PAIR_24_PENALTY=2.629836
PRIMER_LEFT_24_PENALTY=0.288804
PRIMER_RIGHT_24_PENALTY=2.341032
PRIMER_LEFT_24_SEQUENCE=TGAACACTTGGGCCTTTCTT
PRIMER_RIGHT_24_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_24=761,20
PRIMER_RIGHT_24=1547,21
PRIMER_LEFT_24_TM=59.711
PRIMER_RIGHT_24_TM=58.659
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=8.4000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=787
PRIMER_PAIR_25_PENALTY=2.629836
PRIMER_LEFT_25_PENALTY=0.288804
PRIMER_RIGHT_25_PENALTY=2.341032
PRIMER_LEFT_25_SEQUENCE=TTGAACACTTGGGCCTTTCT
PRIMER_RIGHT_25_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_25=760,20
PRIMER_RIGHT_25=1547,21
PRIMER_LEFT_25_TM=59.711
PRIMER_RIGHT_25_TM=58.659
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=788
PRIMER_PAIR_26_PENALTY=2.630633
PRIMER_LEFT_26_PENALTY=0.179421
PRIMER_RIGHT_26_PENALTY=2.451213
PRIMER_LEFT_26_SEQUENCE=CTTGGGCCTTTCTTCTTCCT
PRIMER_RIGHT_26_SEQUENCE=TTGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_26=767,20
PRIMER_RIGHT_26=1551,22
PRIMER_LEFT_26_TM=59.821
PRIMER_RIGHT_26_TM=60.451
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=9.6000
PRIMER_PAIR_26_COMPL_ANY=2.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=785
PRIMER_PAIR_27_PENALTY=2.630807
PRIMER_LEFT_27_PENALTY=0.179594
PRIMER_RIGHT_27_PENALTY=2.451213
PRIMER_LEFT_27_SEQUENCE=TTGGGCCTTTCTTCTTCCTT
PRIMER_RIGHT_27_SEQUENCE=TTGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_27=768,20
PRIMER_RIGHT_27=1551,22
PRIMER_LEFT_27_TM=60.180
PRIMER_RIGHT_27_TM=60.451
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.455
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=9.6000
PRIMER_PAIR_27_COMPL_ANY=2.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=784
PRIMER_PAIR_28_PENALTY=2.650450
PRIMER_LEFT_28_PENALTY=0.309418
PRIMER_RIGHT_28_PENALTY=2.341032
PRIMER_LEFT_28_SEQUENCE=ACTTGGGCCTTTCTTCTTCC
PRIMER_RIGHT_28_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_28=766,20
PRIMER_RIGHT_28=1547,21
PRIMER_LEFT_28_TM=59.691
PRIMER_RIGHT_28_TM=58.659
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=42.857
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.4000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=782
PRIMER_PAIR_29_PENALTY=2.706830
PRIMER_LEFT_29_PENALTY=0.846380
PRIMER_RIGHT_29_PENALTY=1.860449
PRIMER_LEFT_29_SEQUENCE=GCCTGCCATAGGATTTGAGT
PRIMER_RIGHT_29_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_29=708,20
PRIMER_RIGHT_29=1549,21
PRIMER_LEFT_29_TM=59.154
PRIMER_RIGHT_29_TM=59.140
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=842
PRIMER_PAIR_30_PENALTY=2.706830
PRIMER_LEFT_30_PENALTY=0.846380
PRIMER_RIGHT_30_PENALTY=1.860449
PRIMER_LEFT_30_SEQUENCE=GCCTGCCATAGGATTTGAGT
PRIMER_RIGHT_30_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_30=708,20
PRIMER_RIGHT_30=1550,21
PRIMER_LEFT_30_TM=59.154
PRIMER_RIGHT_30_TM=59.140
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=9.6000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=843
PRIMER_PAIR_31_PENALTY=2.740017
PRIMER_LEFT_31_PENALTY=0.288804
PRIMER_RIGHT_31_PENALTY=2.451213
PRIMER_LEFT_31_SEQUENCE=TGAACACTTGGGCCTTTCTT
PRIMER_RIGHT_31_SEQUENCE=TTGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_31=761,20
PRIMER_RIGHT_31=1551,22
PRIMER_LEFT_31_TM=59.711
PRIMER_RIGHT_31_TM=60.451
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=45.455
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=9.6000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=791
PRIMER_PAIR_32_PENALTY=2.740017
PRIMER_LEFT_32_PENALTY=0.288804
PRIMER_RIGHT_32_PENALTY=2.451213
PRIMER_LEFT_32_SEQUENCE=TTGAACACTTGGGCCTTTCT
PRIMER_RIGHT_32_SEQUENCE=TTGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_32=760,20
PRIMER_RIGHT_32=1551,22
PRIMER_LEFT_32_TM=59.711
PRIMER_RIGHT_32_TM=60.451
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=9.6000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=792
PRIMER_PAIR_33_PENALTY=2.760630
PRIMER_LEFT_33_PENALTY=0.309418
PRIMER_RIGHT_33_PENALTY=2.451213
PRIMER_LEFT_33_SEQUENCE=ACTTGGGCCTTTCTTCTTCC
PRIMER_RIGHT_33_SEQUENCE=TTGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_33=766,20
PRIMER_RIGHT_33=1551,22
PRIMER_LEFT_33_TM=59.691
PRIMER_RIGHT_33_TM=60.451
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.2000
PRIMER_RIGHT_33_END_STABILITY=9.6000
PRIMER_PAIR_33_COMPL_ANY=2.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=786
PRIMER_PAIR_34_PENALTY=2.824801
PRIMER_LEFT_34_PENALTY=0.029253
PRIMER_RIGHT_34_PENALTY=2.795548
PRIMER_LEFT_34_SEQUENCE=CATTGAACACTTGGGCCTTT
PRIMER_RIGHT_34_SEQUENCE=TCCGTCTCTATTCGTCCATT
PRIMER_LEFT_34=758,20
PRIMER_RIGHT_34=1546,20
PRIMER_LEFT_34_TM=59.971
PRIMER_RIGHT_34_TM=57.204
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=789
PRIMER_PAIR_35_PENALTY=2.824801
PRIMER_LEFT_35_PENALTY=0.029253
PRIMER_RIGHT_35_PENALTY=2.795548
PRIMER_LEFT_35_SEQUENCE=CATTGAACACTTGGGCCTTT
PRIMER_RIGHT_35_SEQUENCE=ATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_35=758,20
PRIMER_RIGHT_35=1548,20
PRIMER_LEFT_35_TM=59.971
PRIMER_RIGHT_35_TM=57.204
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.5000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=791
PRIMER_PAIR_36_PENALTY=2.829538
PRIMER_LEFT_36_PENALTY=0.969089
PRIMER_RIGHT_36_PENALTY=1.860449
PRIMER_LEFT_36_SEQUENCE=ATTGAACACTTGGGCCTTTC
PRIMER_RIGHT_36_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_36=759,20
PRIMER_RIGHT_36=1549,21
PRIMER_LEFT_36_TM=59.031
PRIMER_RIGHT_36_TM=59.140
PRIMER_LEFT_36_GC_PERCENT=45.000
PRIMER_RIGHT_36_GC_PERCENT=47.619
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=791
PRIMER_PAIR_37_PENALTY=2.829538
PRIMER_LEFT_37_PENALTY=0.969089
PRIMER_RIGHT_37_PENALTY=1.860449
PRIMER_LEFT_37_SEQUENCE=ATTGAACACTTGGGCCTTTC
PRIMER_RIGHT_37_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_37=759,20
PRIMER_RIGHT_37=1550,21
PRIMER_LEFT_37_TM=59.031
PRIMER_RIGHT_37_TM=59.140
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.0000
PRIMER_RIGHT_37_END_STABILITY=9.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=792
PRIMER_PAIR_38_PENALTY=2.836205
PRIMER_LEFT_38_PENALTY=0.495173
PRIMER_RIGHT_38_PENALTY=2.341032
PRIMER_LEFT_38_SEQUENCE=TCATTGAACACTTGGGCCTT
PRIMER_RIGHT_38_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_38=757,20
PRIMER_RIGHT_38=1547,21
PRIMER_LEFT_38_TM=60.495
PRIMER_RIGHT_38_TM=58.659
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=9.7000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=791
PRIMER_PAIR_39_PENALTY=2.870057
PRIMER_LEFT_39_PENALTY=1.009608
PRIMER_RIGHT_39_PENALTY=1.860449
PRIMER_LEFT_39_SEQUENCE=ATTGAACACTTGGGCCTTTCT
PRIMER_RIGHT_39_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_39=759,21
PRIMER_RIGHT_39=1549,21
PRIMER_LEFT_39_TM=59.990
PRIMER_RIGHT_39_TM=59.140
PRIMER_LEFT_39_GC_PERCENT=42.857
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.0000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=791
PRIMER_PAIR_40_PENALTY=2.870057
PRIMER_LEFT_40_PENALTY=1.009608
PRIMER_RIGHT_40_PENALTY=1.860449
PRIMER_LEFT_40_SEQUENCE=ATTGAACACTTGGGCCTTTCT
PRIMER_RIGHT_40_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_40=759,21
PRIMER_RIGHT_40=1550,21
PRIMER_LEFT_40_TM=59.990
PRIMER_RIGHT_40_TM=59.140
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=47.619
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=9.6000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=792
PRIMER_PAIR_41_PENALTY=2.946385
PRIMER_LEFT_41_PENALTY=0.495173
PRIMER_RIGHT_41_PENALTY=2.451213
PRIMER_LEFT_41_SEQUENCE=TCATTGAACACTTGGGCCTT
PRIMER_RIGHT_41_SEQUENCE=TTGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_41=757,20
PRIMER_RIGHT_41=1551,22
PRIMER_LEFT_41_TM=60.495
PRIMER_RIGHT_41_TM=60.451
PRIMER_LEFT_41_GC_PERCENT=45.000
PRIMER_RIGHT_41_GC_PERCENT=45.455
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=9.7000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=795
PRIMER_PAIR_42_PENALTY=2.951689
PRIMER_LEFT_42_PENALTY=1.091240
PRIMER_RIGHT_42_PENALTY=1.860449
PRIMER_LEFT_42_SEQUENCE=CACTTGGGCCTTTCTTCTTC
PRIMER_RIGHT_42_SEQUENCE=GATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_42=765,20
PRIMER_RIGHT_42=1549,21
PRIMER_LEFT_42_TM=58.909
PRIMER_RIGHT_42_TM=59.140
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=785
PRIMER_PAIR_43_PENALTY=2.951689
PRIMER_LEFT_43_PENALTY=1.091240
PRIMER_RIGHT_43_PENALTY=1.860449
PRIMER_LEFT_43_SEQUENCE=CACTTGGGCCTTTCTTCTTC
PRIMER_RIGHT_43_SEQUENCE=TGATTCCGTCTCTATTCGTCC
PRIMER_LEFT_43=765,20
PRIMER_RIGHT_43=1550,21
PRIMER_LEFT_43_TM=58.909
PRIMER_RIGHT_43_TM=59.140
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=9.6000
PRIMER_PAIR_43_COMPL_ANY=2.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=786
PRIMER_PAIR_44_PENALTY=2.974626
PRIMER_LEFT_44_PENALTY=0.633593
PRIMER_RIGHT_44_PENALTY=2.341032
PRIMER_LEFT_44_SEQUENCE=CGTAGACACGCGTATTGGAG
PRIMER_RIGHT_44_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_44=570,20
PRIMER_RIGHT_44=1547,21
PRIMER_LEFT_44_TM=59.366
PRIMER_RIGHT_44_TM=58.659
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=8.2000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=978
PRIMER_PAIR_45_PENALTY=2.974969
PRIMER_LEFT_45_PENALTY=0.179421
PRIMER_RIGHT_45_PENALTY=2.795548
PRIMER_LEFT_45_SEQUENCE=CTTGGGCCTTTCTTCTTCCT
PRIMER_RIGHT_45_SEQUENCE=TCCGTCTCTATTCGTCCATT
PRIMER_LEFT_45=767,20
PRIMER_RIGHT_45=1546,20
PRIMER_LEFT_45_TM=59.821
PRIMER_RIGHT_45_TM=57.204
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=8.4000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=780
PRIMER_PAIR_46_PENALTY=2.974969
PRIMER_LEFT_46_PENALTY=0.179421
PRIMER_RIGHT_46_PENALTY=2.795548
PRIMER_LEFT_46_SEQUENCE=CTTGGGCCTTTCTTCTTCCT
PRIMER_RIGHT_46_SEQUENCE=ATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_46=767,20
PRIMER_RIGHT_46=1548,20
PRIMER_LEFT_46_TM=59.821
PRIMER_RIGHT_46_TM=57.204
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=2.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=8.2000
PRIMER_RIGHT_46_END_STABILITY=7.9000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=782
PRIMER_PAIR_47_PENALTY=2.975143
PRIMER_LEFT_47_PENALTY=0.179594
PRIMER_RIGHT_47_PENALTY=2.795548
PRIMER_LEFT_47_SEQUENCE=TTGGGCCTTTCTTCTTCCTT
PRIMER_RIGHT_47_SEQUENCE=TCCGTCTCTATTCGTCCATT
PRIMER_LEFT_47=768,20
PRIMER_RIGHT_47=1546,20
PRIMER_LEFT_47_TM=60.180
PRIMER_RIGHT_47_TM=57.204
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=2.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=8.4000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=779
PRIMER_PAIR_48_PENALTY=2.975143
PRIMER_LEFT_48_PENALTY=0.179594
PRIMER_RIGHT_48_PENALTY=2.795548
PRIMER_LEFT_48_SEQUENCE=TTGGGCCTTTCTTCTTCCTT
PRIMER_RIGHT_48_SEQUENCE=ATTCCGTCTCTATTCGTCCA
PRIMER_LEFT_48=768,20
PRIMER_RIGHT_48=1548,20
PRIMER_LEFT_48_TM=60.180
PRIMER_RIGHT_48_TM=57.204
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=8.2000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=781
PRIMER_PAIR_49_PENALTY=3.024294
PRIMER_LEFT_49_PENALTY=0.683262
PRIMER_RIGHT_49_PENALTY=2.341032
PRIMER_LEFT_49_SEQUENCE=GGGCCTTTCTTCTTCCTTCA
PRIMER_RIGHT_49_SEQUENCE=TTCCGTCTCTATTCGTCCATT
PRIMER_LEFT_49=770,20
PRIMER_RIGHT_49=1547,21
PRIMER_LEFT_49_TM=60.683
PRIMER_RIGHT_49_TM=58.659
PRIMER_LEFT_49_GC_PERCENT=50.000
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=2.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=8.4000
PRIMER_PAIR_49_COMPL_ANY=2.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=778
=
SEQUENCE_ID=MAGIC.433_1_21173004_21173043_ler-0_can-0_437411_69215_TRUE
SEQUENCE_TARGET=950,139
SEQUENCE_TEMPLATE=ATGAAAAGANGTTTCACCTTNCACATNNGTTCAACGNCGANTCAGNGAACTCCAAGTTACAAAAGCCACATCTGTTCATATTTCACTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCCATTCATATTTGACATTAGCAATATANTGCTCGAATTCAGCAGCNAATACAAAGATTTNGACATCCTTGAATGTGGTGTTCAGATCTTGACGGATGAAACAGATGAAAGAAACATTTGGGGTTCTCTAGTATTCTGNGAAGCATTCGAAAGTGAAGAAGAAGACGTTGATAATCTCACTGACAGGAGACCTGAAGTCGGCGAAACGTCCAACAGAGGCAANNAAGAGGGAGACAAATCTGAATCGGGAGAGGCGTCNNNAGNNNAAGATGAAGANATGGCCGAAGAGGACGACGATGATCACCAACAAGACNATGGTGACTATGNATCCGTATCAAGGAAGCGTCCAAGGATGACGATGATCACAAATCTCAAGGGGNAAATCCAAAAACGTATGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNANAANGCCTTGTGGAAGGACAAAGANCCAATGCGAAGTGAACGANAAAGCTTGGAAAAAAGAAGATAATTTGCATTTNTCANNATAGAAATGCAACATGTAATTACTTTTCTAAACGCTTGTNCTTATCTCCCAATGTAAGAATGCAAATCTTGGAGTCTCCAATAGCACGTANTACTATGTATTTCTTCACTCGAATCATATCCTACTACAATGCGTCCTCTATCTCACTCACGTTNAGGTTNAGGTAAAGTGNAAAGCGTTTCAGGGTAGGNTTCCAANNNNNNNNNNNNNTCGAACCTTCCAATNAAATCAAGCCGTGCACGTAATAACNAAGAGGTTACCANAATTAGGNTTNTTAGATTGATANCTTCCNAGACAAGAAAGAGGACNATCAAAACTACTTTTATCTAGCNNAGANAAGAANACGAANNGNATATNGCCTTTATAGACGNTTANCAGTACANAAGATCNTGATGCCAAGGATCGATNCGCNAACGAGTTGATAAAATCTTGANTGGTGGTGATGGCCGANCAGAGCTTAGAGANGGAGCGAATGCAGGAAGTCTTGAGAGTANCTCAATGGTTAAATCCAGAGGAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_13157002_13157270_mt-0_can-0_344158_14133_TRUE
SEQUENCE_TARGET=950,368
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAATACAATGACTAAACTAATCNNATGANCTATCAGGTGNCACATCNGTTTTATAATTATNACAAGTTTCTACATTTCTTTACTANANNTGTACNATTANAAATTTTCAAATTAAGGAAACTAAATATAATAAAATGTTTAGCATTCATCCGCCTANTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGAAAGGCCCACATGTTTCCTTGAGCAAGAATATAGGTGAAGTGNTTAATTCNCGANNNNNNNNNNNNACNAAGTCAAAGCNANNCNGATTGGAGAGTCNAGTNGGCCGNAAGGTGAANCCCANGAAGTCCGGAGTCAATTCAAATTCGAACGTTTGAATAGTTNATTNGAAGANCGAAANNTANNCATTTAANNNNNNNNNNNNNTGTGTAANTNAGAATTAAANGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNCCATGTNNANANACACGCANTTTTTGTGCAAACNTACTATACACNTTNTNTTNCAAAACTCATAGTTNTNTCTATAATNATCAATCTATTTTCTAGTACAGTTGTCCTCAAGCATAATCCTTCTCACTAAATAAATAAATACNNNNNNNNNNNNNNNNNNNNTTNACATTTTGGCGCCGTCTNTNGGGNTNNAGAATTCTTGNGTCATCNGANNNTNNNTATACTACATTCCCGAAGTGAGCAGCAATGTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_1_13089058_13089379_ct-1_rsch-4_102594_113358_TRUE
SEQUENCE_TARGET=950,421
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGCCCGCCANNCTCTGTGTCTCTTCGTCTAAGGGTTTCTTCCAAAGAACGACGACAAAACCACTGAACCTAAAATCCGAAATCCAAAAGATTCATGCGAAAAANTCGTNAAAGAGTACCAATTTCANAAAGNTACATTCNTNCAGAGANCAANTAATTCCCCAGAAATGGGATCTAGGGTTCCAATAGAAACCATCGAAGAAGACGGCGTAAGTTNAAATCTCTTTCTGCGTTNTTGAATCAANTTTTTNGNTTATTCAACTTCTGGGNTTTTCTCCTACAGGAATTCGATTGGGAAGCAGCAGTCAAAGAAATCGACTTGGCTTGTCTTAAAACCACAAACGCTTCTTCTTCTTCGTCATCCCATTTCACTCCTTTGGCNAATCCACCAATNACGGCAAATCTCACTAAGCCACCTGCGAAGAGACAANCTACTCTCGATAAATTCATCGGCAGAACCGAACATAAACCGGAGAATCATCAAGTTGTTTCCGAGTGTGGTGTTAACGATAACGATAATAGTCCTTTAGTTGGGATTGATCCTGAGGCAGC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_12793950_12793987_po-0_oy-0_30669_542527_TRUE
SEQUENCE_TARGET=950,137
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_16013594_16013862_hi-0_sf-2_641658_40035_TRUE
SEQUENCE_TARGET=950,368
SEQUENCE_TEMPLATE=AAACAAGTGATCAAGTCGGTGAGAAGCGACTAACCCTTACAAAGTCTTGTAAAATATATGATAAAAGTCTTGCGTAGTTAAACTTTTTGCCTCTTTCGACGTTGGTGCCAATATTAAACAGTGTAAAGACCCAACGCGTTTTAACTNTCCACGAATAGGATTATTTTAGGATCAANGTCTACGGAAGTAGATAATGGAGAAAATGNTAAACNAAAAGACCAGTCTTAAAACCTTTCGTGTAGCNAACACAACACTAGACCAAAATTGACTCAAATTATAATTTAACATATTTGATCAAATGTTAACTCGGGTACACACNNATATACAAACAACACATTATCTGATTTTGTTATTATTTATGTCGTTTTATTTTAAAAAGATAAATAAAAGAGAAAGAGAATTCTACTAAATACATTTATATTATTGTTTAACAAGTTAATATTTGTATATCGAATTGTGAAATTTATCAAATGAATACATATTTATATATTTTTATAGCTTGTAACANATATCCACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_15343596_15343836_po-0_can-0_26312_38401_TRUE
SEQUENCE_TARGET=950,340
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGANNGTNGTTNTGGTTATTGNTATTGTGAAGAAGATGAAAGTNATAAGACAANTATAAGAAACACANACGANTTTTAAGNANATANGGAAGAGAAGGTGTGNGAAGGGGAAAAAGGNTTTTGATGAAAGGTTNNTTGTGGATTATATAGAANCCAAATCTGAAGNATCNNANATTTGNAANNNCANANNNTGAAGNAGTGNGGTGGNGGATTCTCNAAGCNCANCNNCNNAGNCNTGTCANATNGTTGAGTTGTNGCTAGCTNTNNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGTTCACTGAGCATATGAGGGAATCAATTGATGATGNATGATGTGGTGGTAGTGGTGTTAATTTNTCGTTAAGTAAATGTTTGGATCANNTACNCAGAAAANGGCNATCATATGATNTGAATTACNTTTTAGNANAGCNTGTNAAGTTTTGATCNTTTAGGGTTTTAGATNTAAAATTAACANAGCTCTNTGATTTNACATTTNGGAAATTGAATCNCATGTTAATGTAGATGAAGAATCTANCCANGTGTNTTGAAATATGNTTGNGAANNNAAAAACTGATTTATTNATTTTTGNAGGAANNNTGATAGTTGGTTTTGTTACATTTGAGAACGTAGAACAATTGNAGAGNNNCNTGTAATTGATTTTGTTNGTTTGTNTTGANCTTATTTATTTTGNNATATANCAAAGAANTNANNNACNTNANCAACAAATTNGTCTTANAGTTCNGNTTATTTATTNTATTCTNNNATAGATTATGTTGGCTTAAGAAGNCATTTAGACTTNAGAGTTNTTTCNTCTGNTAAANTANATGTCCTTCATTNTTNCAAANGATTAAGTGATTTTNAGTTNNNNNNNNNAAATGATTAAATNNNNCTGAGNTTTGTTCTTCCAAATNAAATNAAAAANATTAANAACNTAATCNNTTCTTAAGAAACNGATTANCNAATNTCAAGAAATANATATTTGTANACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAATTTGGAACGANAGATTGTTTGNCTACCAACAACATNAANNAAANNNNNNNCAAANGGATATAGTTCNAAAATAANTAATTTTATATGGTCAAAACTACNNTTGCTATATAAAGNNCAAANACTATAGAAACNCAACTCAACAATAATNATCCNNTTNCTTTNNNNNTTTTTTTGCTATNNNNNCTAAGCAAANTTTTCATAAGTTTGAACANTCATAAANTCATCTCTTCNAAATAATGNCAANTCAAACAATAAAAAAGATATANAGTNTTTTGATNATTGTAGTTTTATTTACGATGATGNTTTCAACCAACGCGAGTACGGTGGAANTNTGTGTTAAGCANTGNGTCCCGAANCAATGNATGAAAGTTTCTCAGAAGGCAACTCTTCCATTATGTGAGACTGCTTGCACAAAACTNTGCAATGAAAANAAACATGAGGAATATGTCGTGCCNCGNAGTTACTNNGNNGGATTATTNTGGTTTNTATGTGATAANTGAAANTTAATAAATATGCAAAGTAATATATATNTATTTTTTATCTNTNNAAAAATAATNTATNATGTNAAAATGTACCTTTAATCAATATACTAATTTCATGTAATTATTTTATNTATACTAATNA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_3_13167824_13167860_wu-0_no-0_8291_479662_TRUE
SEQUENCE_TARGET=950,136
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_12219901_12220135_oy-0_rsch-4_296563_26996_TRUE
SEQUENCE_TARGET=950,334
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCCTTCTCGTCTTGCAGAACACTTCGACCCCTTGACTNNCGAGGTCAAGAATGGGCATCATACAATCTGGGATGTGNGCAAATTNTGCAATTCCAAGTTCGATCNAATNTNTNGGGTNGGAGTTGAGGNTGTGTAACTCCCTTGATATCACNGTGACCCTCCGCAGCATTTGGTTGATCTTCTTTCGAAGCTTCTTCTTTTNCGTTGAGCNTTTTTTGTGTTGAGCNGCGAGAGCTTTGATAAAGGCCATCGCGATANCTTNATCTCTGNTNNANAGGTCCTTGATTAATGCACACGGAGCTNGGATCTGNGATCTNAAGTTGCGAAGTGGGGAGCGCCCTCTGTCTGGTACNTTCGGAATGTCCTNATCGATCATCCCGATCCTCNAAAAGTCTNACANGGNTTCCGAACATCGGAGGTTNAAGGACATAACTGCACAAGAAAAGAACGATATTGTNAGTGAGNANTGAAGNAAAACCTAGAGAGGTAATTTATAGGGAGGTTTCTTAGCTTTCAAAGATGNGATTCCATTTAAAGTCGGAGTNATTCGCNGAAGCATTCANGTNGCCTTNTTNNCCCTGCATTCAAAGAGTATCTCCAAAACGAATATTTGATGNNNNNNNNAAAGTTTGNAANTTTNNTTAAATAAAATGGGCCGAAGCCCNATAANAAAGTCCATANGAAAANAAAAAAAGTTTAANTCTCAGGGAACATCTAGGGAAGGGGCTCAGGAACAGGCTCNTTCATCGGCGNTTGAGGTGGACNCCTCGTTCTTAACATCCACCTCGGGTTCGTTCTTCACGCGCTTCNAAGGNCTCGAAGCCATNGCTNGACAGAAACAATGGAGTTNCGGGATCGANGGGGCAAACNGTGGNGGNTCCTTNGTTTTCCATTCTAGAGGTGTTTACTAAGTAAATTTNTGAGATCATATTTTGAGGGAGTTTAGNTTATNCCCTTATTTATAGTGATTTAGGGTTTTACTNGAAANCACAAANTTAATANTTGTGGCGTGTCGGAATNAGTTTATCCATTTTTGGG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_8345276_8345725_ws-0_can-0_53147_13512_TRUE
SEQUENCE_TARGET=950,549
SEQUENCE_TEMPLATE=TTATAGGAGGGATCTAGGCCCGTATCAGGCCCATTGCAAGAGGAAATTCAAAATTTTGTCTTCTTTTGGCCCAAATCAATTTAATAAAATCTGAATTAGGGGTTTTGTTTTTTGATTTCATGATATAAACGACAAAAACCAAANGTACATATATAACCAGAACTCAAGAGTCTTTTATAATAAAGGNAAACCGAAAAAACAGAACCGGTTATCAAGTCTCTCTCTTCCTTTTCTATCCAAATAACATCCANTTGTAATAATCTTATCGACAAACAATCGACCTATAACTACCCACGTGTCANCACGATACGATTATTATCCTCTCTGATCCAACGGACCCAAAACATCTATCTCTCTTTCTCGACCTTTTGTCTCCNCGATCTAAAGATGGCGGATTCGGACAACGATTCAGGAGGACACAAAGACGGTGGAAATGCTTCGACACGTGAGCAAGATAGGTTTCTACCGATCGCTAACGTTAGCAGGATCATGAAGAAAGCACTTCCNGCGAACGCAAAAATCTCNAAGGATGCTAAAGAAACGGTTCAAGAGTGTGTATCGGAATTCATAAGTTTCATCACCGGTGANGCTTCTGACAAGTGTCAGAGAGAGAAGAGGAAGACAATCAACGGTGANGATCTTCTTTGGGCGATGACTACGCTAGGGTTTGANGANTACGTGGAGCCTCTCAAGGTTTATCTGCAAAAGTATAGGGAGGTGGAAGGAGAGAAGACTACTACGGCNGGGAGACAAGGCGATAAGGAAGGTGGAGGAGGAGGCGGTGGAGCTGGAAGTGGAAGTGGAGGAGCTCCGATGTACGGTGGTGGCATGGTGACTACGATGGGNCATCAATTTTCCCATCATTTTTCTTAATTGTAAAATGATAAAAGCAAATTTTCATTTTTNTTAATTAATNNNNNNTATATATATATGTTTAACTTTTAGTATANTGTTTACAGNNTTTNTTNTTNNNAACTAGGTTCAACCCACNNNNNTAACANAATAAATATTAAATTTATGATTCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGTTAGGGTGTTTATTCGTGAAAGATGAAAGTAACAAAANTAAACNAGGATATCGAAGTTTNTGGTNGAATTAGGANGAANAATCAAATGAGAGAGACANACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTTGTNATNCGAAAATTTNATATTTGAGTCCAGGTTTTTNAGAGCTTGNNTTTTCCATTAGTCCNNNNNATGCCACAAATATTCNAAGATTTTTTCTTACAAGCAATACTCAAATATCAAAACTAAGTTTCTATNAAAAAANNAAACAAAACNTCAGCCAGGTAAAATCAGGAAAAAATAAAGAATTATCAACAAATACCATGACTACAATAACCAAACTGCTTTCCATTNTTNTTTTTGTCAAGCAAATTGTATTCAACATTGAATTTCAAAACAGAGAAGATCATACNGTGTAGAATTAAAAACAAGATTGAAACGATACATTANAAATNACGACAAANGCTTGAAAATTTATCAGNTAGGAAGNTCCGACGAANCTAAATGAANGTCTCCATNACANACTCCANANTGAANACTANTTTCAAAAGATTAAAGANAAACTCATGATNATAATAGCTCGTACAGCTANTGCTTCTTCCATCGAAGNAGNNCTTTCAAGNAATCACTCCAAAAAGGAGTTGTANAGGAAGNATCTNTCCAAAACANTCCAAAGCAANACAATAGGAGAAGCNTTTACCCTTTTNGNGTCGTGAAGGACCATGCTTTGTGCTCTGTTCTCACAACATTGAGAACTNTGTAGTAGAGCGAAGCTGGNAAAGAGTTGTGGCGTTGTGGATGCATNTGGTACTCTNATGCCAAACCTCGTAAACACACGCATNACATGCTTGAANCANTGCAATGGAGATGTCATTGCTTNTAGAAGTTGTTGATGACNNNCNGNNGGTGCTGGAATCATTCAATAGNNNNNNNNGTAGGCAANAACTTTACCATAATTNTGTTNCAGATNTGAGCTGAAAATGAATAATTTAAGTGCAAGTGGTGNNTTCCTAGATTCNCCCTCAANGCCACATTAAAGCAAGTTTGTTCAACATGACATNNCAANAAAAATCCTNTCAATAGTAGGGTTGAGGTTAAAAATAAAGAGTTATAAACTGCGAAANCATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.492464
PRIMER_LEFT_0_PENALTY=0.185942
PRIMER_RIGHT_0_PENALTY=1.306521
PRIMER_LEFT_0_SEQUENCE=GGGAGGTGGAAGGAGAGAAG
PRIMER_RIGHT_0_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_0=712,20
PRIMER_RIGHT_0=1617,21
PRIMER_LEFT_0_TM=60.186
PRIMER_RIGHT_0_TM=59.693
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=1.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=906
PRIMER_PAIR_1_PENALTY=1.535702
PRIMER_LEFT_1_PENALTY=0.229181
PRIMER_RIGHT_1_PENALTY=1.306521
PRIMER_LEFT_1_SEQUENCE=TGGAAGTGGAAGTGGAGGAG
PRIMER_RIGHT_1_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_1=788,20
PRIMER_RIGHT_1=1617,21
PRIMER_LEFT_1_TM=60.229
PRIMER_RIGHT_1_TM=59.693
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=830
PRIMER_PAIR_2_PENALTY=1.535702
PRIMER_LEFT_2_PENALTY=0.229181
PRIMER_RIGHT_2_PENALTY=1.306521
PRIMER_LEFT_2_SEQUENCE=CTGGAAGTGGAAGTGGAGGA
PRIMER_RIGHT_2_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_2=787,20
PRIMER_RIGHT_2=1617,21
PRIMER_LEFT_2_TM=60.229
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=831
PRIMER_PAIR_3_PENALTY=1.671841
PRIMER_LEFT_3_PENALTY=0.365320
PRIMER_RIGHT_3_PENALTY=1.306521
PRIMER_LEFT_3_SEQUENCE=GATCTTCTTTGGGCGATGAC
PRIMER_RIGHT_3_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_3=636,20
PRIMER_RIGHT_3=1617,21
PRIMER_LEFT_3_TM=59.635
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=982
PRIMER_PAIR_4_PENALTY=1.692277
PRIMER_LEFT_4_PENALTY=0.385756
PRIMER_RIGHT_4_PENALTY=1.306521
PRIMER_LEFT_4_SEQUENCE=GAGCTGGAAGTGGAAGTGGA
PRIMER_RIGHT_4_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_4=784,20
PRIMER_RIGHT_4=1617,21
PRIMER_LEFT_4_TM=60.386
PRIMER_RIGHT_4_TM=59.693
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=834
PRIMER_PAIR_5_PENALTY=1.856870
PRIMER_LEFT_5_PENALTY=0.550349
PRIMER_RIGHT_5_PENALTY=1.306521
PRIMER_LEFT_5_SEQUENCE=AGCTGGAAGTGGAAGTGGAG
PRIMER_RIGHT_5_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_5=785,20
PRIMER_RIGHT_5=1617,21
PRIMER_LEFT_5_TM=59.450
PRIMER_RIGHT_5_TM=59.693
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=833
PRIMER_PAIR_6_PENALTY=1.888240
PRIMER_LEFT_6_PENALTY=0.185942
PRIMER_RIGHT_6_PENALTY=1.702298
PRIMER_LEFT_6_SEQUENCE=GGGAGGTGGAAGGAGAGAAG
PRIMER_RIGHT_6_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_6=712,20
PRIMER_RIGHT_6=1616,20
PRIMER_LEFT_6_TM=60.186
PRIMER_RIGHT_6_TM=58.298
PRIMER_LEFT_6_GC_PERCENT=60.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=1.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=905
PRIMER_PAIR_7_PENALTY=1.888240
PRIMER_LEFT_7_PENALTY=0.185942
PRIMER_RIGHT_7_PENALTY=1.702298
PRIMER_LEFT_7_SEQUENCE=AGGGAGGTGGAAGGAGAGAA
PRIMER_RIGHT_7_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_7=711,20
PRIMER_RIGHT_7=1616,20
PRIMER_LEFT_7_TM=60.186
PRIMER_RIGHT_7_TM=58.298
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=45.000
PRIMER_LEFT_7_SELF_ANY=1.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=906
PRIMER_PAIR_8_PENALTY=1.896184
PRIMER_LEFT_8_PENALTY=0.589662
PRIMER_RIGHT_8_PENALTY=1.306521
PRIMER_LEFT_8_SEQUENCE=AGGCGATAAGGAAGGTGGAG
PRIMER_RIGHT_8_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_8=752,20
PRIMER_RIGHT_8=1617,21
PRIMER_LEFT_8_TM=60.590
PRIMER_RIGHT_8_TM=59.693
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PAIR_8_PRODUCT_SIZE=866
PRIMER_PAIR_9_PENALTY=1.931478
PRIMER_LEFT_9_PENALTY=0.229181
PRIMER_RIGHT_9_PENALTY=1.702298
PRIMER_LEFT_9_SEQUENCE=TGGAAGTGGAAGTGGAGGAG
PRIMER_RIGHT_9_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_9=788,20
PRIMER_RIGHT_9=1616,20
PRIMER_LEFT_9_TM=60.229
PRIMER_RIGHT_9_TM=58.298
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=1.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=829
PRIMER_PAIR_10_PENALTY=1.931478
PRIMER_LEFT_10_PENALTY=0.229181
PRIMER_RIGHT_10_PENALTY=1.702298
PRIMER_LEFT_10_SEQUENCE=CTGGAAGTGGAAGTGGAGGA
PRIMER_RIGHT_10_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_10=787,20
PRIMER_RIGHT_10=1616,20
PRIMER_LEFT_10_TM=60.229
PRIMER_RIGHT_10_TM=58.298
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=830
PRIMER_PAIR_11_PENALTY=1.974617
PRIMER_LEFT_11_PENALTY=0.185942
PRIMER_RIGHT_11_PENALTY=1.788675
PRIMER_LEFT_11_SEQUENCE=GGGAGGTGGAAGGAGAGAAG
PRIMER_RIGHT_11_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_11=712,20
PRIMER_RIGHT_11=1618,21
PRIMER_LEFT_11_TM=60.186
PRIMER_RIGHT_11_TM=59.211
PRIMER_LEFT_11_GC_PERCENT=60.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=1.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=907
PRIMER_PAIR_12_PENALTY=2.001934
PRIMER_LEFT_12_PENALTY=0.695412
PRIMER_RIGHT_12_PENALTY=1.306521
PRIMER_LEFT_12_SEQUENCE=GTGGAGCTGGAAGTGGAAGT
PRIMER_RIGHT_12_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_12=781,20
PRIMER_RIGHT_12=1617,21
PRIMER_LEFT_12_TM=59.305
PRIMER_RIGHT_12_TM=59.693
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=42.857
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PAIR_12_PRODUCT_SIZE=837
PRIMER_PAIR_13_PENALTY=2.017856
PRIMER_LEFT_13_PENALTY=0.229181
PRIMER_RIGHT_13_PENALTY=1.788675
PRIMER_LEFT_13_SEQUENCE=TGGAAGTGGAAGTGGAGGAG
PRIMER_RIGHT_13_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_13=788,20
PRIMER_RIGHT_13=1618,21
PRIMER_LEFT_13_TM=60.229
PRIMER_RIGHT_13_TM=59.211
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=1.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=831
PRIMER_PAIR_14_PENALTY=2.017856
PRIMER_LEFT_14_PENALTY=0.229181
PRIMER_RIGHT_14_PENALTY=1.788675
PRIMER_LEFT_14_SEQUENCE=CTGGAAGTGGAAGTGGAGGA
PRIMER_RIGHT_14_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_14=787,20
PRIMER_RIGHT_14=1618,21
PRIMER_LEFT_14_TM=60.229
PRIMER_RIGHT_14_TM=59.211
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=832
PRIMER_PAIR_15_PENALTY=2.037986
PRIMER_LEFT_15_PENALTY=0.731465
PRIMER_RIGHT_15_PENALTY=1.306521
PRIMER_LEFT_15_SEQUENCE=GGAGACAAGGCGATAAGGAA
PRIMER_RIGHT_15_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_15=745,20
PRIMER_RIGHT_15=1617,21
PRIMER_LEFT_15_TM=59.269
PRIMER_RIGHT_15_TM=59.693
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=42.857
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PAIR_15_PRODUCT_SIZE=873
PRIMER_PAIR_16_PENALTY=2.067618
PRIMER_LEFT_16_PENALTY=0.365320
PRIMER_RIGHT_16_PENALTY=1.702298
PRIMER_LEFT_16_SEQUENCE=GATCTTCTTTGGGCGATGAC
PRIMER_RIGHT_16_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_16=636,20
PRIMER_RIGHT_16=1616,20
PRIMER_LEFT_16_TM=59.635
PRIMER_RIGHT_16_TM=58.298
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=45.000
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.3000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=981
PRIMER_PAIR_17_PENALTY=2.088053
PRIMER_LEFT_17_PENALTY=0.385756
PRIMER_RIGHT_17_PENALTY=1.702298
PRIMER_LEFT_17_SEQUENCE=GAGCTGGAAGTGGAAGTGGA
PRIMER_RIGHT_17_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_17=784,20
PRIMER_RIGHT_17=1616,20
PRIMER_LEFT_17_TM=60.386
PRIMER_RIGHT_17_TM=58.298
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=833
PRIMER_PAIR_18_PENALTY=2.111997
PRIMER_LEFT_18_PENALTY=0.805475
PRIMER_RIGHT_18_PENALTY=1.306521
PRIMER_LEFT_18_SEQUENCE=ACAAGGCGATAAGGAAGGTG
PRIMER_RIGHT_18_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_18=749,20
PRIMER_RIGHT_18=1617,21
PRIMER_LEFT_18_TM=59.195
PRIMER_RIGHT_18_TM=59.693
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=869
PRIMER_PAIR_19_PENALTY=2.153995
PRIMER_LEFT_19_PENALTY=0.365320
PRIMER_RIGHT_19_PENALTY=1.788675
PRIMER_LEFT_19_SEQUENCE=GATCTTCTTTGGGCGATGAC
PRIMER_RIGHT_19_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_19=636,20
PRIMER_RIGHT_19=1618,21
PRIMER_LEFT_19_TM=59.635
PRIMER_RIGHT_19_TM=59.211
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.3000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=983
PRIMER_PAIR_20_PENALTY=2.174431
PRIMER_LEFT_20_PENALTY=0.385756
PRIMER_RIGHT_20_PENALTY=1.788675
PRIMER_LEFT_20_SEQUENCE=GAGCTGGAAGTGGAAGTGGA
PRIMER_RIGHT_20_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_20=784,20
PRIMER_RIGHT_20=1618,21
PRIMER_LEFT_20_TM=60.386
PRIMER_RIGHT_20_TM=59.211
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=9.7000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=835
PRIMER_PAIR_21_PENALTY=2.252646
PRIMER_LEFT_21_PENALTY=0.550349
PRIMER_RIGHT_21_PENALTY=1.702298
PRIMER_LEFT_21_SEQUENCE=AGCTGGAAGTGGAAGTGGAG
PRIMER_RIGHT_21_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_21=785,20
PRIMER_RIGHT_21=1616,20
PRIMER_LEFT_21_TM=59.450
PRIMER_RIGHT_21_TM=58.298
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=45.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.2000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=832
PRIMER_PAIR_22_PENALTY=2.255235
PRIMER_LEFT_22_PENALTY=0.948713
PRIMER_RIGHT_22_PENALTY=1.306521
PRIMER_LEFT_22_SEQUENCE=AAGGCGATAAGGAAGGTGGA
PRIMER_RIGHT_22_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_22=751,20
PRIMER_RIGHT_22=1617,21
PRIMER_LEFT_22_TM=60.949
PRIMER_RIGHT_22_TM=59.693
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=42.857
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.9000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=6.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=867
PRIMER_PAIR_23_PENALTY=2.291960
PRIMER_LEFT_23_PENALTY=0.589662
PRIMER_RIGHT_23_PENALTY=1.702298
PRIMER_LEFT_23_SEQUENCE=AGGCGATAAGGAAGGTGGAG
PRIMER_RIGHT_23_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_23=752,20
PRIMER_RIGHT_23=1616,20
PRIMER_LEFT_23_TM=60.590
PRIMER_RIGHT_23_TM=58.298
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=8.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=865
PRIMER_PAIR_24_PENALTY=2.339024
PRIMER_LEFT_24_PENALTY=0.550349
PRIMER_RIGHT_24_PENALTY=1.788675
PRIMER_LEFT_24_SEQUENCE=AGCTGGAAGTGGAAGTGGAG
PRIMER_RIGHT_24_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_24=785,20
PRIMER_RIGHT_24=1618,21
PRIMER_LEFT_24_TM=59.450
PRIMER_RIGHT_24_TM=59.211
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=42.857
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=9.7000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=834
PRIMER_PAIR_25_PENALTY=2.363315
PRIMER_LEFT_25_PENALTY=0.185942
PRIMER_RIGHT_25_PENALTY=2.177372
PRIMER_LEFT_25_SEQUENCE=GGGAGGTGGAAGGAGAGAAG
PRIMER_RIGHT_25_SEQUENCE=TTCCTGATTTTACCTGGCTG
PRIMER_LEFT_25=712,20
PRIMER_RIGHT_25=1617,20
PRIMER_LEFT_25_TM=60.186
PRIMER_RIGHT_25_TM=57.823
PRIMER_LEFT_25_GC_PERCENT=60.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=1.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=6.7000
PRIMER_RIGHT_25_END_STABILITY=9.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=906
PRIMER_PAIR_26_PENALTY=2.378337
PRIMER_LEFT_26_PENALTY=0.589662
PRIMER_RIGHT_26_PENALTY=1.788675
PRIMER_LEFT_26_SEQUENCE=AGGCGATAAGGAAGGTGGAG
PRIMER_RIGHT_26_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_26=752,20
PRIMER_RIGHT_26=1618,21
PRIMER_LEFT_26_TM=60.590
PRIMER_RIGHT_26_TM=59.211
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=6.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=867
PRIMER_PAIR_27_PENALTY=2.397710
PRIMER_LEFT_27_PENALTY=0.695412
PRIMER_RIGHT_27_PENALTY=1.702298
PRIMER_LEFT_27_SEQUENCE=GTGGAGCTGGAAGTGGAAGT
PRIMER_RIGHT_27_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_27=781,20
PRIMER_RIGHT_27=1616,20
PRIMER_LEFT_27_TM=59.305
PRIMER_RIGHT_27_TM=58.298
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=836
PRIMER_PAIR_28_PENALTY=2.406553
PRIMER_LEFT_28_PENALTY=0.229181
PRIMER_RIGHT_28_PENALTY=2.177372
PRIMER_LEFT_28_SEQUENCE=TGGAAGTGGAAGTGGAGGAG
PRIMER_RIGHT_28_SEQUENCE=TTCCTGATTTTACCTGGCTG
PRIMER_LEFT_28=788,20
PRIMER_RIGHT_28=1617,20
PRIMER_LEFT_28_TM=60.229
PRIMER_RIGHT_28_TM=57.823
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=45.000
PRIMER_LEFT_28_SELF_ANY=1.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=9.7000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=830
PRIMER_PAIR_29_PENALTY=2.406553
PRIMER_LEFT_29_PENALTY=0.229181
PRIMER_RIGHT_29_PENALTY=2.177372
PRIMER_LEFT_29_SEQUENCE=CTGGAAGTGGAAGTGGAGGA
PRIMER_RIGHT_29_SEQUENCE=TTCCTGATTTTACCTGGCTG
PRIMER_LEFT_29=787,20
PRIMER_RIGHT_29=1617,20
PRIMER_LEFT_29_TM=60.229
PRIMER_RIGHT_29_TM=57.823
PRIMER_LEFT_29_GC_PERCENT=55.000
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=831
PRIMER_PAIR_30_PENALTY=2.433763
PRIMER_LEFT_30_PENALTY=0.731465
PRIMER_RIGHT_30_PENALTY=1.702298
PRIMER_LEFT_30_SEQUENCE=GGAGACAAGGCGATAAGGAA
PRIMER_RIGHT_30_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_30=745,20
PRIMER_RIGHT_30=1616,20
PRIMER_LEFT_30_TM=59.269
PRIMER_RIGHT_30_TM=58.298
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PAIR_30_PRODUCT_SIZE=872
PRIMER_PAIR_31_PENALTY=2.484087
PRIMER_LEFT_31_PENALTY=0.695412
PRIMER_RIGHT_31_PENALTY=1.788675
PRIMER_LEFT_31_SEQUENCE=GTGGAGCTGGAAGTGGAAGT
PRIMER_RIGHT_31_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_31=781,20
PRIMER_RIGHT_31=1618,21
PRIMER_LEFT_31_TM=59.305
PRIMER_RIGHT_31_TM=59.211
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=838
PRIMER_PAIR_32_PENALTY=2.493112
PRIMER_LEFT_32_PENALTY=1.186590
PRIMER_RIGHT_32_PENALTY=1.306521
PRIMER_LEFT_32_SEQUENCE=TAGGGAGGTGGAAGGAGAGA
PRIMER_RIGHT_32_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_32=710,20
PRIMER_RIGHT_32=1617,21
PRIMER_LEFT_32_TM=58.813
PRIMER_RIGHT_32_TM=59.693
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=42.857
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=908
PRIMER_PAIR_33_PENALTY=2.507773
PRIMER_LEFT_33_PENALTY=0.805475
PRIMER_RIGHT_33_PENALTY=1.702298
PRIMER_LEFT_33_SEQUENCE=ACAAGGCGATAAGGAAGGTG
PRIMER_RIGHT_33_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_33=749,20
PRIMER_RIGHT_33=1616,20
PRIMER_LEFT_33_TM=59.195
PRIMER_RIGHT_33_TM=58.298
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=45.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=868
PRIMER_PAIR_34_PENALTY=2.520140
PRIMER_LEFT_34_PENALTY=0.731465
PRIMER_RIGHT_34_PENALTY=1.788675
PRIMER_LEFT_34_SEQUENCE=GGAGACAAGGCGATAAGGAA
PRIMER_RIGHT_34_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_34=745,20
PRIMER_RIGHT_34=1618,21
PRIMER_LEFT_34_TM=59.269
PRIMER_RIGHT_34_TM=59.211
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=42.857
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.2000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=874
PRIMER_PAIR_35_PENALTY=2.520916
PRIMER_LEFT_35_PENALTY=1.214394
PRIMER_RIGHT_35_PENALTY=1.306521
PRIMER_LEFT_35_SEQUENCE=GAGACAAGGCGATAAGGAAGG
PRIMER_RIGHT_35_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_35=746,21
PRIMER_RIGHT_35=1617,21
PRIMER_LEFT_35_TM=60.214
PRIMER_RIGHT_35_TM=59.693
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=8.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=872
PRIMER_PAIR_36_PENALTY=2.520916
PRIMER_LEFT_36_PENALTY=1.214394
PRIMER_RIGHT_36_PENALTY=1.306521
PRIMER_LEFT_36_SEQUENCE=GGAGACAAGGCGATAAGGAAG
PRIMER_RIGHT_36_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_36=745,21
PRIMER_RIGHT_36=1617,21
PRIMER_LEFT_36_TM=60.214
PRIMER_RIGHT_36_TM=59.693
PRIMER_LEFT_36_GC_PERCENT=52.381
PRIMER_RIGHT_36_GC_PERCENT=42.857
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=8.2000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=873
PRIMER_PAIR_37_PENALTY=2.542693
PRIMER_LEFT_37_PENALTY=0.365320
PRIMER_RIGHT_37_PENALTY=2.177372
PRIMER_LEFT_37_SEQUENCE=GATCTTCTTTGGGCGATGAC
PRIMER_RIGHT_37_SEQUENCE=TTCCTGATTTTACCTGGCTG
PRIMER_LEFT_37=636,20
PRIMER_RIGHT_37=1617,20
PRIMER_LEFT_37_TM=59.635
PRIMER_RIGHT_37_TM=57.823
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=3.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=6.3000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=982
PRIMER_PAIR_38_PENALTY=2.563128
PRIMER_LEFT_38_PENALTY=0.385756
PRIMER_RIGHT_38_PENALTY=2.177372
PRIMER_LEFT_38_SEQUENCE=GAGCTGGAAGTGGAAGTGGA
PRIMER_RIGHT_38_SEQUENCE=TTCCTGATTTTACCTGGCTG
PRIMER_LEFT_38=784,20
PRIMER_RIGHT_38=1617,20
PRIMER_LEFT_38_TM=60.386
PRIMER_RIGHT_38_TM=57.823
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=834
PRIMER_PAIR_39_PENALTY=2.563602
PRIMER_LEFT_39_PENALTY=1.257080
PRIMER_RIGHT_39_PENALTY=1.306521
PRIMER_LEFT_39_SEQUENCE=ATCTTCTTTGGGCGATGACT
PRIMER_RIGHT_39_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_39=637,20
PRIMER_RIGHT_39=1617,21
PRIMER_LEFT_39_TM=58.743
PRIMER_RIGHT_39_TM=59.693
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=42.857
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=3.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=981
PRIMER_PAIR_40_PENALTY=2.567868
PRIMER_LEFT_40_PENALTY=1.261347
PRIMER_RIGHT_40_PENALTY=1.306521
PRIMER_LEFT_40_SEQUENCE=CGATAAGGAAGGTGGAGGAG
PRIMER_RIGHT_40_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_40=755,20
PRIMER_RIGHT_40=1617,21
PRIMER_LEFT_40_TM=58.739
PRIMER_RIGHT_40_TM=59.693
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=42.857
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=6.00
PRIMER_PAIR_40_COMPL_END=1.00
PRIMER_PAIR_40_PRODUCT_SIZE=863
PRIMER_PAIR_41_PENALTY=2.594150
PRIMER_LEFT_41_PENALTY=0.805475
PRIMER_RIGHT_41_PENALTY=1.788675
PRIMER_LEFT_41_SEQUENCE=ACAAGGCGATAAGGAAGGTG
PRIMER_RIGHT_41_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_41=749,20
PRIMER_RIGHT_41=1618,21
PRIMER_LEFT_41_TM=59.195
PRIMER_RIGHT_41_TM=59.211
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=42.857
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=9.7000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=870
PRIMER_PAIR_42_PENALTY=2.648146
PRIMER_LEFT_42_PENALTY=0.185942
PRIMER_RIGHT_42_PENALTY=2.462203
PRIMER_LEFT_42_SEQUENCE=GGGAGGTGGAAGGAGAGAAG
PRIMER_RIGHT_42_SEQUENCE=TTTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_42=712,20
PRIMER_RIGHT_42=1619,22
PRIMER_LEFT_42_TM=60.186
PRIMER_RIGHT_42_TM=60.462
PRIMER_LEFT_42_GC_PERCENT=60.000
PRIMER_RIGHT_42_GC_PERCENT=40.909
PRIMER_LEFT_42_SELF_ANY=1.00
PRIMER_RIGHT_42_SELF_ANY=3.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=9.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=908
PRIMER_PAIR_43_PENALTY=2.651011
PRIMER_LEFT_43_PENALTY=0.948713
PRIMER_RIGHT_43_PENALTY=1.702298
PRIMER_LEFT_43_SEQUENCE=AAGGCGATAAGGAAGGTGGA
PRIMER_RIGHT_43_SEQUENCE=TCCTGATTTTACCTGGCTGA
PRIMER_LEFT_43=751,20
PRIMER_RIGHT_43=1616,20
PRIMER_LEFT_43_TM=60.949
PRIMER_RIGHT_43_TM=58.298
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=3.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.9000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=3.00
PRIMER_PAIR_43_PRODUCT_SIZE=866
PRIMER_PAIR_44_PENALTY=2.691384
PRIMER_LEFT_44_PENALTY=0.229181
PRIMER_RIGHT_44_PENALTY=2.462203
PRIMER_LEFT_44_SEQUENCE=TGGAAGTGGAAGTGGAGGAG
PRIMER_RIGHT_44_SEQUENCE=TTTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_44=788,20
PRIMER_RIGHT_44=1619,22
PRIMER_LEFT_44_TM=60.229
PRIMER_RIGHT_44_TM=60.462
PRIMER_LEFT_44_GC_PERCENT=55.000
PRIMER_RIGHT_44_GC_PERCENT=40.909
PRIMER_LEFT_44_SELF_ANY=1.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=9.7000
PRIMER_PAIR_44_COMPL_ANY=4.00
PRIMER_PAIR_44_COMPL_END=1.00
PRIMER_PAIR_44_PRODUCT_SIZE=832
PRIMER_PAIR_45_PENALTY=2.691384
PRIMER_LEFT_45_PENALTY=0.229181
PRIMER_RIGHT_45_PENALTY=2.462203
PRIMER_LEFT_45_SEQUENCE=CTGGAAGTGGAAGTGGAGGA
PRIMER_RIGHT_45_SEQUENCE=TTTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_45=787,20
PRIMER_RIGHT_45=1619,22
PRIMER_LEFT_45_TM=60.229
PRIMER_RIGHT_45_TM=60.462
PRIMER_LEFT_45_GC_PERCENT=55.000
PRIMER_RIGHT_45_GC_PERCENT=40.909
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=3.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=833
PRIMER_PAIR_46_PENALTY=2.696461
PRIMER_LEFT_46_PENALTY=1.389940
PRIMER_RIGHT_46_PENALTY=1.306521
PRIMER_LEFT_46_SEQUENCE=TGGAGCTGGAAGTGGAAGTG
PRIMER_RIGHT_46_SEQUENCE=TTCCTGATTTTACCTGGCTGA
PRIMER_LEFT_46=782,20
PRIMER_RIGHT_46=1617,21
PRIMER_LEFT_46_TM=61.390
PRIMER_RIGHT_46_TM=59.693
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=4.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=836
PRIMER_PAIR_47_PENALTY=2.727721
PRIMER_LEFT_47_PENALTY=0.550349
PRIMER_RIGHT_47_PENALTY=2.177372
PRIMER_LEFT_47_SEQUENCE=AGCTGGAAGTGGAAGTGGAG
PRIMER_RIGHT_47_SEQUENCE=TTCCTGATTTTACCTGGCTG
PRIMER_LEFT_47=785,20
PRIMER_RIGHT_47=1617,20
PRIMER_LEFT_47_TM=59.450
PRIMER_RIGHT_47_TM=57.823
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=45.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=833
PRIMER_PAIR_48_PENALTY=2.737388
PRIMER_LEFT_48_PENALTY=0.948713
PRIMER_RIGHT_48_PENALTY=1.788675
PRIMER_LEFT_48_SEQUENCE=AAGGCGATAAGGAAGGTGGA
PRIMER_RIGHT_48_SEQUENCE=TTTCCTGATTTTACCTGGCTG
PRIMER_LEFT_48=751,20
PRIMER_RIGHT_48=1618,21
PRIMER_LEFT_48_TM=60.949
PRIMER_RIGHT_48_TM=59.211
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=3.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=9.7000
PRIMER_PAIR_48_COMPL_ANY=6.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=868
PRIMER_PAIR_49_PENALTY=2.767035
PRIMER_LEFT_49_PENALTY=0.589662
PRIMER_RIGHT_49_PENALTY=2.177372
PRIMER_LEFT_49_SEQUENCE=AGGCGATAAGGAAGGTGGAG
PRIMER_RIGHT_49_SEQUENCE=TTCCTGATTTTACCTGGCTG
PRIMER_LEFT_49=752,20
PRIMER_RIGHT_49=1617,20
PRIMER_LEFT_49_TM=60.590
PRIMER_RIGHT_49_TM=57.823
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=8.2000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=6.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=866
=
SEQUENCE_ID=MAGIC.149_4_8290911_8291242_edi-0_wil-2_236936_330308_TRUE
SEQUENCE_TARGET=950,431
SEQUENCE_TEMPLATE=NNGAACANACNTACCNATGAACATACACGNTTCACAAGANTTNTCTCAAAGCGGANGAGATGGCNNNANGCCACNTACNGGAGACACCNAATCNGTGCNANANTNCCTNNCTAACCTNAGATTNTCGGATNTNTNCNCTNTNCNTNNTNAAGATGTCCNGATGANTCCNNNGNTNTTTAGNNAAATNAGTNATGGGAANAANGNATGTCTGGANNNGTTGAGAAGCCNNNNNNNNNNNNNNNNANGTNTCAAGAGCNANACAGGAGATTCNNTTCTTCANCTTGCTGNTANATGGGGTCATNTAGAACTAGTGAAGGAGATTGTCTNTGAATGNCNNNNNCTTCTNTTGGAGCNAAACTNNAGCGNTCAGACACCNCTNCATGTGGCGGCNNATNGCGGNCATACCNCTNTTNTTGAGNCTTTTGTNGCATTGGTAACATTTNCNTCAGCTANANNGNGTANNGAAGANAGTGAGAGAATGAANCCNTATGTTCTCAAGGACNANGATGGAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGTCTGGTGAANGCNAACNNGGATGCTCCTTTTNTTGNANNNNNNNNNNNNNTATCTNCCNNNNNNNNNNNNNNNNNNNCTGGAGNNGTATCNCTTGTNANNNNNNNNNTGNNNNNNNNNNNNNNNNAGGACCTTNAAGGGANGANNNNNNNNNNNNAGTCNAAGNTACAAGGNCNNAAATNTCTTGCACATGTTGCTTTNNTNNNNNNGAGTATAGGTTTNTTTTTCNCTTTAACCANNNNNNNNNNNNNNNNNNNNNNNCAANNATGTAAAACNAGTTTGAGANGGGANNNNCCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNATTNNTNGANNAGNTGTNCTTGATGNTATTCTTNANGAATNTCCAANTCNNNNNGANGANNNNNNNNNNNNNGGTNGGACTTGTCTTTCANTNGNAGNNNNNNNNNNNTATTATNANGGAGTNTGCAACCTCTTAGANCGATCAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACATCTNGGTGTTGNNCAAGATGTGGACGGGAATACACNNNNNNNNCTTGCCGTCATGAACTGGCACTTNNANTCCANTACTTGNCTTGCNAGNAGTAGCAAGATACTGNANNNNNNNAACAAAANNGGCTTAAGAGCTAGGGATATTGCCGAGANAGAGGTGAAACCCCACTACATNTTTNAGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANANCACATANATGNTTTANNNNNNNNNNCCCANNTTTTGNNTTANAGAGGTGGACANTGGCNCTCTTNTTANACNNNANTCACTNAAGNGGTTTCGAATCTGTNNANTCNTTAACAANACCGNCAGNNCCACTAGACCCTAANAATAACAGAGATTACGTNAACACTCTTNTNNTGGTAGCNGCTCTTGTAGCNACNATGACGTTTGCTGCAGGTTTTACNNTACCNGGTGGNTNTAANAGCTCTGCTCCANACTTGGGCAGGGCNNCTTTGGCCACTAANCCAACTCTCTNNATTTTTCTGNTACTTGACATCTTGGCNATGCAAAGTTCCNTTGCAACNATAGGTANTCTTATTTGGGCGCAGTTNGGTGATCCAGNNCTCATNCGNTCATCCTNNCATGTGGCNTTGCCCTTACTGCTTTTTGCTTTACTCTGCATGCCCNTGGCATTCCTTTTTGGNGTGNTNACNGCANTTGGNCANGTGAAATGGCTTNTAGTCATCATTTGCNTTATATCTNNTNTATTCNNNTNTNGGGCGATCTTTGTCCTTGGNCCNCACGTCATGCTACANCGNNCATACNTTTCTCCCANNTANNNNNNNNNCTTTCTCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTTNTGGTTGTTNNTNAGANTTTGTNGNNTATNNNNNNNNNNNCNNCGTATGAANAAATNATNNNNNNNNNNNNNNNNNNNNNAGATTTTTGNNNNNNTNNANAANAAAANNAAAAAGCTAGATATNATGNTCTCTCTGAGNAGCGGNATCTTNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_7681325_7681393_hi-0_oy-0_102075_217802_TRUE
SEQUENCE_TARGET=950,168
SEQUENCE_TEMPLATE=TNNNTTTTTTTCTTTTCTTTNTTNTACGTNANNNNNAANAAAANTGTACACGATGAAAATGAGGAAANTGTTGTGCCNAATCACGAATTAGTACCCATGTNNGTTNATGCTCTANCAATTCNNTCCANAAGACCACAACTCCANAAGTAACCNNCGCTATGTTNTCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGANCCAAATTTTNCATGAGNANAACTGTTAGCTAANTTAGGGTCTGGNTTAGAACNCAAGTCCAACTTNCAAAANTNAAAANAACTAACTTAGTNNGGTTACGGGTTCTTGGGAATACNTTTNTAGTCGAAAGAACTGTTTTCTCGATCNAAATTTTTGGTAANACATGAATGAAAGAAAGGTGANCTCCGACGAAAAATTNATAACCTAATTTATCATGAGNTTTTGTAGACTNNNNNNNNTTTNACTAAANTNGTGTATTTANGACTNGGTAATCAATTAANTATGTNCAGAATTTTTGGTTGTTNGGATTGACTTTTGGGTAAAANAATTTAGAATTGCAAANAATTNNANACAATCGGTTTGTTNNGNTTAGGTAAAAGCATTCNGNATTGAAAACCTAAGTTNGTNTTGGACCCCNAAAATTTNNNNNNANTTTATCAATTTCGGTACATTTGCAAAGTTNNGTNCAATTCAAANNTTCGGTNTAGACCAAAAGTTNGAANANTNAAAANAAAACATCTGAAAGTTGTNGAATTTNTTTTTNAAGCAAANTGGTAAACCATAAGAACTTNTTTNNNAGTCCACCACATCATTTATCCNTCAAAATCTCCCCAAATACGAAATCAAGCAAGTCTTCCNAAATTTAGAATCTCNAATACACCATGTTGGGAAACAANCCAACTTTNNACATCNNAANATTATANATATATTNNNNNNTATATNAATCAANTCCAACTCNANNNNNNNNNNNNNNNNNNNNNNNNNNAGNCCTAAACAAATTTGTGNAANTTTTACCACTTGGNNCNAAAGCGGACAATATCATNCNAATNGNAANAGTTGGAATGGGCTTNGAGAGCCCAACAACCNTNTTTTTTAGAAAANAATAAANTTATTTGCAAAACGACATGAAAATCATCGTACNAAANATTCAAGATATNTGATGAATTANTTGNTTATTACCANNTCANTAGTTATNAAGATAAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTACACGTCAGNTATTTAGATAANATTNCACAGTACANGTNAGTTATAAAGATNAGATTTCACANTACANNTNAGTTATAAAGATNAGATTTCACANTACANGTNANTTATAAAGATAAGATTTCANNGTACACNTTAGTTATAAAGATAAGATTTCANAATACACGTCAGCCNTAACNCAAAACATANTAGGGTTTGATGTGTACCTATAAGTANAGAGGTTTAAAGCAGAANGAGCAAAACAAAAAAATCTTAGAGACTCACGCCGCTTNGAAGANTTCCNAACATNTCAAGAGATCTNTAAGATTTGGCAATCCAAACANCATTANAANCTAAANAACACAAAATANTTTTTNTTACCAATCTGTAAAANTNTCTCGATCAGAAACNNTTAAAATTTACTCAAACACNATCAAATCCCAATTATTNTNTTTCAACNTNATAAACTGCGGTTCCANACATATNCTCCNACNAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.105_3_14044913_14045584_ler-0_tsu-0_7509_410155_TRUE
SEQUENCE_TARGET=950,771
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_12935518_12936289_sf-2_ws-0_107983_44283_TRUE
SEQUENCE_TARGET=950,871
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14037649_14037795_kn-0_ler-0_11252_30152_TRUE
SEQUENCE_TARGET=950,246
SEQUENCE_TEMPLATE=TNNCCTCAAAGGNGTGAAGCTTTTTCNTGTTTNTCGAGAAAGGANATGNCAANNGTCGCATNAAANACNAAAANCCCATTAAATNNNNNNNNNANAGTTNAGGCNCAAGCCNANNNAANAAGCNGAANTGATTAAACCNGAGTNGGGGANTTCGNNNNNNNAANCGAGCTCGGTTCTCGCNTTTTAAGAGAAAGGTNNNNNNNNNNNNAAAATCTNGAAACTTAGNNACANGGCNANAAGNTNTNTAAAGACGGACTNTNGNAANATNATAGNNNNAAAAGGTTTAAAAGCCTCCTCNGGCAANTACTTNTTNAAAAATNNAGCNTCNNNNNNNNNCANCNNATTTATCCAAATTTAATTTCTAGTTTTGGAAGTGTTTGGGANAGCCCCNAATTAAAGTAGATACAACCACTAGGAATCCGTAATCTTCTCGGGGTCAACGATAGCCNGATCGGCTTCGGTTGGNTCAACAACAGCTGACTCAGTTTTGGTTGGTTCACNGGCAGAGAGTAGCTTAGAAGAGTTTCCCGGCTCAGTTCCGGCTTCATGTTCAACATCCGGGATGATCACTGTCTCACTNTTCACGANCGGACAGTAGTATCGACCANTTCACTAGAGCCTGAAGNGAACGTTTCAAGTCAGCAANAAACACAAATACAACAACTNNNGNNNATAANGNTNCAGATNTAAAAGNTTANCANCCCAATCTAAGCCTGATTCACTCAAANNCANNGNNNNGNNNTAANANTCCTCGAAGTGTTGAGGGTTAAAGGACAAGGTGGGAAGAGNAGCAAGGTGGCCTCTTTTCTCAGCTAAAACAGCCTCCAGTTTGGACCTTTCTTGTTCCTCAGAGAAATCTTCCTCAAGGGTTTGGTCCAACANAAGCAGGNTNCTTTCGTGCTNNTNNNNNNNNNNNTNNAGCTCCGATNTGGCNNTCTCNGTTTGAATGANNAGGATAGCNCCTTGNATCANNTCCATNCCGCGACNTTTCACNTCTCTTNNNNNNNNNNNNNNNNNNNTGGCTAATTCAGCATCNNANNNNNNNNNNNNNNNNTTGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNAGTCGAGAGTTCNTCCTNNAGCTTGTCNTANNTTTGNTACTGATCNTGCAACTCGCCATTNGANNTCATGAGCTTNNNNNNNAGATGNTCGNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAGAGGAGGAGCNNNNNNNNNNNNNNNGNGAAGANAANTTGCCTTTCCAAGCATCNATNTCCNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNGATCATGTNNTTAAGCTCNNTGTTCGCCTNAGCAANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACCCGANATGAATGATGNGCNTGGAGTAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGATNGTGTNTTTCCNNGTTTTGAAGCNNNGNAANACGTTGCCCTCTTTCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCGAGNGAAATNAANCCNTNGTTNGAACCTNTAAGCNATTTNGTNTGAGGAACGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTNGATCAGNCTTCTCNCCACTAGGTNGNNGAGAAGGNNNNNNNNNNNNNNNNGAGGAGGAGNACCCATCGATCNAGATGGAGGAGATGATCGNNGAGAGCGATCAGTNCGAGCCNTTGACTTTTCCCTCCAAGGAGAAGAGCTGTTNCGAGCTGGTCTCTCNCTCNNTNTNNNNATNAAGTCAGCGATTGGCTTGCTGCTCAGTCCATCACGGGCTNAAGTCCCGTGTGGTNTGGGAGGANGNGGTGGAAGATGATGGAGTTGTCCAGGAGGACTCATNTNGAGNNNNNNNNNNNNNNNNNNNNNNNNGTCGAGGGTAAACAAATTCCAAAGGGTTTCACCTTCGATGACCACTTTGAAGAANNNNNNNNATTCTTCAGTCGGAAGNGNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_3_14469493_14470166_oy-0_sf-2_6192_2189_TRUE
SEQUENCE_TARGET=950,773
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANANTANAATTCAGGATGATGNNGCTGATAANAATCAATTGAGGANAAAGAGGAAGNGNAAAGTTNTTGACGNTTGCCCCNNGAAAGTNNNGACNTATCCNGAACAAGAAAAAAACAGTNGGAGTTTNGTCAAGTTTNNATTTTAANNAACTTAAGATTTTTGGTGTTGAATGAGTNAGAACNTGTTGAAGAAGACCTGGAAAATGANAAAAGAGAGGACNANATANAAGGTTTGGAAGAAGAGGANNANCTNGAAGATAAAGTANANGATAAAANAAGGNNGGGNNNTGTAACAGAGATGGANAAGGNTAATAAGGATGCANANATAACTCTNAANATGNAANTCAAANAGGNAAAGTCGCTCCNNCAAGNCCATCACTACACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTGCAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_10040991_10041009_hi-0_tsu-0_1590700_20278_TRUE
SEQUENCE_TARGET=950,118
SEQUENCE_TEMPLATE=AATNTTATTACTCCATGCATTTAGTTCATNNAAAAAATATCTNTATANTAATATNNNGAAAGTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNCATAAGATCNATTTGTTTTTCTCGCGGNTTANAGATCTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNATGAATCTNGTACCTATTCAATGGNGTTANCAAGGANCACNNTACTCNAAAAGGAGCCATAGATAACCTCCTTANCGCNAAATCATTTTGGTTAATGAANGGACTATTCTGGAGAGACTTTGNNCTCTANCNTANAAACNTGGTGTTGCNAATGAAGNTNTANAGNTATATTANTGTATANTTATTGCNTTTATTTGGANTNTTTTAGAAATNAATTCATATTTNTTTTTNANTTTTGNTAACTAGATCAATGCNTCAAGATTCAANGTAGTNNATCTNATTGTTCATANAGTAGAANACTATGAAAATCTTACCAANNTTAACAAANATTTATTTCGNTNNNNNNNNNNNNNNNNNNNNGNNTTGTTGTTNNAGTNTTTNAGNCCTTTNGAAATATTGGAANAANGANGAGTANAAGANTANATTAANATGGTTATGNATTTNTAATTTATATCTCACTTNNTTGAGATAAAANNGTTNNATTNTACGCCNNAAAACCNTAAGNTGANGTTCAAGCATGTANATNNNNNNNNNNNNNNNCTGNACAATAGTTACGTTGANGNCATANCATTGTAAAATGTTAGCTNACTAGGCAAGTNNNNNNNNNNNNNNNTTTTGGAANNNNNNNNNNNNTTTTAANGGTTCTAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_3_6147291_6147339_wu-0_bur-0_860258_98_TRUE
SEQUENCE_TARGET=950,148
SEQUENCE_TEMPLATE=TGANACCAGCGATTACAGAGATGCAAGATCCTCATGTCCCATCTTCTCCAACTATCTCCTCCGTCTCTTCCTCCGATCTTGATACTGAGGTCAGTTCCTGCAATCTTATACTTAATAGATAATCTAATTAAAGATCAAGNNCTTCATGTGTTTTCTAAGCTACNCNNNNNNNNNNATGCTATAAAGTATCAACCTTTAATTAGAAAACTAGTTTAAAGTNCCAAAGGTTATGTTTTCAGAACTGCCCAGAAACATATATATGTACTTGTTAGAACTTGATTTNNTTTTTTNATTGGTGATGTGAGGATTTAGTCNACAGGATCGTTCTTCCATGACAGAAGCATCACACTTGGAACGCTTATGGGATTTAGTTTCACNGCAACTATGCCGATGCCATTNAGAGCNTCTTCACGCCGCCACGTGTCACCTTCCGTCGCAATCTCACGAGCTTCTAGCTCCAATGCAAGACGCAACCACCAGAGAAAGCGTCCTCCTTCAAATTCGGCTGAGCCTGAGCCGCATCGCCGCCGTAAATGGTGGCGGTTTTGCCGAGATGACGATGACGACGCCGCCGGAAATGGAATCCACCGTGGAACAGGAGATTCAAAGCGGTCATCGCTNGGGGAGTATNTNGAGGTGGAACGGAGGTTTGGAGATGAAGCCGTTNACAACTCCGCCGAGGCAGAGCTAGAGGATGCGGTGGTGGCACGGTACCAAGATCAGCAGCCGGTGATGGGGGAGCGGGCATTGTTCGCTGACGGAAGGGTTCTTCCTCCGGCTTCAGNTGAAGNGGTTACCGGAGAANGNACGCCGGTNGCCACGNCCCTTTGTAGATTTCCGGNNTCTTTAACCNGTATATGCAGCGGTGGNGGAGGNTGAATACGTCGGTTCATGTGTGNGNNNNNNATTTGTATTTAGACTTAAATTTTTGTATNNNNNNNNNNNNNNNNGAATCATTGTTTGTGGAAAATATAACATGTTGNATGTAGTCATTGTCAAANTAATTATTTGAGANNNAANNNNNNNTNNNAAGGAGAATTANNAANNANANTANNNANANNNGNAATANTNNNNNNTNNNCNTNNANNANNNNANACNTGNANANCTNTNGNTNNNAANTGGAGNNNNNCNCTNNATNANNNTNTTNATTTNAAATGNTAANNGTTGNTCTATCANATTGAGTTTTTGCTTCAAANNNNNNNNNNNNNNNNTCTTTATACTAGTCAGTCTTTGGACAATAATTTGACCAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANGAGTGTGCCACATTNGTGGTTANTTTTCCACTAGTATTTCTGAAAGTAAAACAATTAACCACAAATCATCCTCATAACATAAGAGGAGAAGATTCCTAACGACGAAGCAAAATTATCGAACAAGTGGACACACACAAGAAACCGAAAATACGGCAAAATGTTTGTTATGTGTATCCATATTTTATANCAATTGAATTAATAAAATAAACTGTANAAACAAGAAACAATTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAANCCTCGAGTGNTGTTAAAAAAAGGAAGAAGGAAAAAGAATAAAAACTCAAAGGGAACGCAACACACTNAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTNNNCAGCAGCAGCAGNAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGCTGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCACCGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCTGAATGATTTTTTCCTTGGGTAGAGTCAAAATTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.106709
PRIMER_LEFT_0_PENALTY=0.049859
PRIMER_RIGHT_0_PENALTY=0.056850
PRIMER_LEFT_0_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_0_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_0=639,20
PRIMER_RIGHT_0=1444,20
PRIMER_LEFT_0_TM=60.050
PRIMER_RIGHT_0_TM=59.943
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.6000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=806
PRIMER_PAIR_1_PENALTY=0.243451
PRIMER_LEFT_1_PENALTY=0.186600
PRIMER_RIGHT_1_PENALTY=0.056850
PRIMER_LEFT_1_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_1_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_1=592,20
PRIMER_RIGHT_1=1444,20
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=853
PRIMER_PAIR_2_PENALTY=0.311463
PRIMER_LEFT_2_PENALTY=0.254613
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_2_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_2=677,20
PRIMER_RIGHT_2=1444,20
PRIMER_LEFT_2_TM=60.255
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=768
PRIMER_PAIR_3_PENALTY=0.410901
PRIMER_LEFT_3_PENALTY=0.254613
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_LEFT_3_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_3_SEQUENCE=GTGTTGCGTTCCCTTTGAGT
PRIMER_LEFT_3=677,20
PRIMER_RIGHT_3=1646,20
PRIMER_LEFT_3_TM=60.255
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=970
PRIMER_PAIR_4_PENALTY=0.422170
PRIMER_LEFT_4_PENALTY=0.365320
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_4_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_4=597,20
PRIMER_RIGHT_4=1444,20
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=848
PRIMER_PAIR_5_PENALTY=0.463402
PRIMER_LEFT_5_PENALTY=0.049859
PRIMER_RIGHT_5_PENALTY=0.413543
PRIMER_LEFT_5_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_5_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_5=639,20
PRIMER_RIGHT_5=1441,20
PRIMER_LEFT_5_TM=60.050
PRIMER_RIGHT_5_TM=59.586
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=45.000
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=803
PRIMER_PAIR_6_PENALTY=0.463402
PRIMER_LEFT_6_PENALTY=0.049859
PRIMER_RIGHT_6_PENALTY=0.413543
PRIMER_LEFT_6_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_6_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_6=639,20
PRIMER_RIGHT_6=1442,20
PRIMER_LEFT_6_TM=60.050
PRIMER_RIGHT_6_TM=59.586
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=804
PRIMER_PAIR_7_PENALTY=0.463402
PRIMER_LEFT_7_PENALTY=0.049859
PRIMER_RIGHT_7_PENALTY=0.413543
PRIMER_LEFT_7_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_7_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_7=639,20
PRIMER_RIGHT_7=1443,20
PRIMER_LEFT_7_TM=60.050
PRIMER_RIGHT_7_TM=59.586
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=805
PRIMER_PAIR_8_PENALTY=0.478724
PRIMER_LEFT_8_PENALTY=0.049859
PRIMER_RIGHT_8_PENALTY=0.428866
PRIMER_LEFT_8_SEQUENCE=GAACGGAGGTTTGGAGATGA
PRIMER_RIGHT_8_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_8=639,20
PRIMER_RIGHT_8=1432,20
PRIMER_LEFT_8_TM=60.050
PRIMER_RIGHT_8_TM=59.571
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=794
PRIMER_PAIR_9_PENALTY=0.530362
PRIMER_LEFT_9_PENALTY=0.254613
PRIMER_RIGHT_9_PENALTY=0.275749
PRIMER_LEFT_9_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_9_SEQUENCE=TGCGTTCCCTTTGAGTTTTT
PRIMER_LEFT_9=677,20
PRIMER_RIGHT_9=1642,20
PRIMER_LEFT_9_TM=60.255
PRIMER_RIGHT_9_TM=59.724
PRIMER_LEFT_9_GC_PERCENT=60.000
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=966
PRIMER_PAIR_10_PENALTY=0.530362
PRIMER_LEFT_10_PENALTY=0.254613
PRIMER_RIGHT_10_PENALTY=0.275749
PRIMER_LEFT_10_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_10_SEQUENCE=TTGCGTTCCCTTTGAGTTTT
PRIMER_LEFT_10=677,20
PRIMER_RIGHT_10=1643,20
PRIMER_LEFT_10_TM=60.255
PRIMER_RIGHT_10_TM=59.724
PRIMER_LEFT_10_GC_PERCENT=60.000
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=967
PRIMER_PAIR_11_PENALTY=0.530545
PRIMER_LEFT_11_PENALTY=0.473695
PRIMER_RIGHT_11_PENALTY=0.056850
PRIMER_LEFT_11_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_11_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_11=640,20
PRIMER_RIGHT_11=1444,20
PRIMER_LEFT_11_TM=59.526
PRIMER_RIGHT_11_TM=59.943
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=805
PRIMER_PAIR_12_PENALTY=0.567181
PRIMER_LEFT_12_PENALTY=0.510331
PRIMER_RIGHT_12_PENALTY=0.056850
PRIMER_LEFT_12_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_12_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_12=586,20
PRIMER_RIGHT_12=1444,20
PRIMER_LEFT_12_TM=60.510
PRIMER_RIGHT_12_TM=59.943
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=859
PRIMER_PAIR_13_PENALTY=0.600143
PRIMER_LEFT_13_PENALTY=0.186600
PRIMER_RIGHT_13_PENALTY=0.413543
PRIMER_LEFT_13_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_13_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_13=592,20
PRIMER_RIGHT_13=1441,20
PRIMER_LEFT_13_TM=59.813
PRIMER_RIGHT_13_TM=59.586
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=11.4000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=850
PRIMER_PAIR_14_PENALTY=0.600143
PRIMER_LEFT_14_PENALTY=0.186600
PRIMER_RIGHT_14_PENALTY=0.413543
PRIMER_LEFT_14_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_14_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_14=592,20
PRIMER_RIGHT_14=1442,20
PRIMER_LEFT_14_TM=59.813
PRIMER_RIGHT_14_TM=59.586
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=40.000
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=11.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=851
PRIMER_PAIR_15_PENALTY=0.600143
PRIMER_LEFT_15_PENALTY=0.186600
PRIMER_RIGHT_15_PENALTY=0.413543
PRIMER_LEFT_15_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_15_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_15=592,20
PRIMER_RIGHT_15=1443,20
PRIMER_LEFT_15_TM=59.813
PRIMER_RIGHT_15_TM=59.586
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=11.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=852
PRIMER_PAIR_16_PENALTY=0.615466
PRIMER_LEFT_16_PENALTY=0.186600
PRIMER_RIGHT_16_PENALTY=0.428866
PRIMER_LEFT_16_SEQUENCE=GAACAGGAGATTCAAAGCGG
PRIMER_RIGHT_16_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_16=592,20
PRIMER_RIGHT_16=1432,20
PRIMER_LEFT_16_TM=59.813
PRIMER_RIGHT_16_TM=59.571
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=11.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=841
PRIMER_PAIR_17_PENALTY=0.635218
PRIMER_LEFT_17_PENALTY=0.578368
PRIMER_RIGHT_17_PENALTY=0.056850
PRIMER_LEFT_17_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_17_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_17=487,20
PRIMER_RIGHT_17=1444,20
PRIMER_LEFT_17_TM=60.578
PRIMER_RIGHT_17_TM=59.943
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=40.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=11.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=958
PRIMER_PAIR_18_PENALTY=0.668156
PRIMER_LEFT_18_PENALTY=0.254613
PRIMER_RIGHT_18_PENALTY=0.413543
PRIMER_LEFT_18_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_18_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_18=677,20
PRIMER_RIGHT_18=1441,20
PRIMER_LEFT_18_TM=60.255
PRIMER_RIGHT_18_TM=59.586
PRIMER_LEFT_18_GC_PERCENT=60.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=765
PRIMER_PAIR_19_PENALTY=0.668156
PRIMER_LEFT_19_PENALTY=0.254613
PRIMER_RIGHT_19_PENALTY=0.413543
PRIMER_LEFT_19_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_19_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_19=677,20
PRIMER_RIGHT_19=1442,20
PRIMER_LEFT_19_TM=60.255
PRIMER_RIGHT_19_TM=59.586
PRIMER_LEFT_19_GC_PERCENT=60.000
PRIMER_RIGHT_19_GC_PERCENT=40.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=766
PRIMER_PAIR_20_PENALTY=0.668156
PRIMER_LEFT_20_PENALTY=0.254613
PRIMER_RIGHT_20_PENALTY=0.413543
PRIMER_LEFT_20_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_20_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_20=677,20
PRIMER_RIGHT_20=1443,20
PRIMER_LEFT_20_TM=60.255
PRIMER_RIGHT_20_TM=59.586
PRIMER_LEFT_20_GC_PERCENT=60.000
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.1000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=767
PRIMER_PAIR_21_PENALTY=0.683479
PRIMER_LEFT_21_PENALTY=0.254613
PRIMER_RIGHT_21_PENALTY=0.428866
PRIMER_LEFT_21_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_21_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_21=677,20
PRIMER_RIGHT_21=1432,20
PRIMER_LEFT_21_TM=60.255
PRIMER_RIGHT_21_TM=59.571
PRIMER_LEFT_21_GC_PERCENT=60.000
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=8.1000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=756
PRIMER_PAIR_22_PENALTY=0.768641
PRIMER_LEFT_22_PENALTY=0.711791
PRIMER_RIGHT_22_PENALTY=0.056850
PRIMER_LEFT_22_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_22_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_22=594,20
PRIMER_RIGHT_22=1444,20
PRIMER_LEFT_22_TM=59.288
PRIMER_RIGHT_22_TM=59.943
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=851
PRIMER_PAIR_23_PENALTY=0.778863
PRIMER_LEFT_23_PENALTY=0.365320
PRIMER_RIGHT_23_PENALTY=0.413543
PRIMER_LEFT_23_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_23_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_23=597,20
PRIMER_RIGHT_23=1441,20
PRIMER_LEFT_23_TM=59.635
PRIMER_RIGHT_23_TM=59.586
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.6000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=845
PRIMER_PAIR_24_PENALTY=0.778863
PRIMER_LEFT_24_PENALTY=0.365320
PRIMER_RIGHT_24_PENALTY=0.413543
PRIMER_LEFT_24_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_24_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_24=597,20
PRIMER_RIGHT_24=1442,20
PRIMER_LEFT_24_TM=59.635
PRIMER_RIGHT_24_TM=59.586
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.6000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=846
PRIMER_PAIR_25_PENALTY=0.778863
PRIMER_LEFT_25_PENALTY=0.365320
PRIMER_RIGHT_25_PENALTY=0.413543
PRIMER_LEFT_25_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_25_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_25=597,20
PRIMER_RIGHT_25=1443,20
PRIMER_LEFT_25_TM=59.635
PRIMER_RIGHT_25_TM=59.586
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=3.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.6000
PRIMER_RIGHT_25_END_STABILITY=7.0000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=847
PRIMER_PAIR_26_PENALTY=0.794186
PRIMER_LEFT_26_PENALTY=0.365320
PRIMER_RIGHT_26_PENALTY=0.428866
PRIMER_LEFT_26_SEQUENCE=GGAGATTCAAAGCGGTCATC
PRIMER_RIGHT_26_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_26=597,20
PRIMER_RIGHT_26=1432,20
PRIMER_LEFT_26_TM=59.635
PRIMER_RIGHT_26_TM=59.571
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.6000
PRIMER_RIGHT_26_END_STABILITY=7.9000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=836
PRIMER_PAIR_27_PENALTY=0.887238
PRIMER_LEFT_27_PENALTY=0.473695
PRIMER_RIGHT_27_PENALTY=0.413543
PRIMER_LEFT_27_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_27_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_27=640,20
PRIMER_RIGHT_27=1441,20
PRIMER_LEFT_27_TM=59.526
PRIMER_RIGHT_27_TM=59.586
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.9000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=802
PRIMER_PAIR_28_PENALTY=0.887238
PRIMER_LEFT_28_PENALTY=0.473695
PRIMER_RIGHT_28_PENALTY=0.413543
PRIMER_LEFT_28_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_28_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_28=640,20
PRIMER_RIGHT_28=1442,20
PRIMER_LEFT_28_TM=59.526
PRIMER_RIGHT_28_TM=59.586
PRIMER_LEFT_28_GC_PERCENT=45.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.9000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=803
PRIMER_PAIR_29_PENALTY=0.887238
PRIMER_LEFT_29_PENALTY=0.473695
PRIMER_RIGHT_29_PENALTY=0.413543
PRIMER_LEFT_29_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_29_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_29=640,20
PRIMER_RIGHT_29=1443,20
PRIMER_LEFT_29_TM=59.526
PRIMER_RIGHT_29_TM=59.586
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=804
PRIMER_PAIR_30_PENALTY=0.889843
PRIMER_LEFT_30_PENALTY=0.832993
PRIMER_RIGHT_30_PENALTY=0.056850
PRIMER_LEFT_30_SEQUENCE=CAACCACCAGAGAAAGCGTC
PRIMER_RIGHT_30_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_30=470,20
PRIMER_RIGHT_30=1444,20
PRIMER_LEFT_30_TM=60.833
PRIMER_RIGHT_30_TM=59.943
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=40.000
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.6000
PRIMER_RIGHT_30_END_STABILITY=6.7000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=975
PRIMER_PAIR_31_PENALTY=0.889843
PRIMER_LEFT_31_PENALTY=0.832993
PRIMER_RIGHT_31_PENALTY=0.056850
PRIMER_LEFT_31_SEQUENCE=GACGCAACCACCAGAGAAAG
PRIMER_RIGHT_31_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_31=466,20
PRIMER_RIGHT_31=1444,20
PRIMER_LEFT_31_TM=60.833
PRIMER_RIGHT_31_TM=59.943
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.0000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=979
PRIMER_PAIR_32_PENALTY=0.889843
PRIMER_LEFT_32_PENALTY=0.832993
PRIMER_RIGHT_32_PENALTY=0.056850
PRIMER_LEFT_32_SEQUENCE=AAGACGCAACCACCAGAGAA
PRIMER_RIGHT_32_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_32=464,20
PRIMER_RIGHT_32=1444,20
PRIMER_LEFT_32_TM=60.833
PRIMER_RIGHT_32_TM=59.943
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.7000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=981
PRIMER_PAIR_33_PENALTY=0.902560
PRIMER_LEFT_33_PENALTY=0.473695
PRIMER_RIGHT_33_PENALTY=0.428866
PRIMER_LEFT_33_SEQUENCE=AACGGAGGTTTGGAGATGAA
PRIMER_RIGHT_33_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_33=640,20
PRIMER_RIGHT_33=1432,20
PRIMER_LEFT_33_TM=59.526
PRIMER_RIGHT_33_TM=59.571
PRIMER_LEFT_33_GC_PERCENT=45.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=793
PRIMER_PAIR_34_PENALTY=0.919829
PRIMER_LEFT_34_PENALTY=0.862979
PRIMER_RIGHT_34_PENALTY=0.056850
PRIMER_LEFT_34_SEQUENCE=ACGGAGGTTTGGAGATGAAG
PRIMER_RIGHT_34_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_34=641,20
PRIMER_RIGHT_34=1444,20
PRIMER_LEFT_34_TM=59.137
PRIMER_RIGHT_34_TM=59.943
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=804
PRIMER_PAIR_35_PENALTY=0.922928
PRIMER_LEFT_35_PENALTY=0.254613
PRIMER_RIGHT_35_PENALTY=0.668315
PRIMER_LEFT_35_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_35_SEQUENCE=TGTTGCGTTCCCTTTGAGTT
PRIMER_LEFT_35=677,20
PRIMER_RIGHT_35=1645,20
PRIMER_LEFT_35_TM=60.255
PRIMER_RIGHT_35_TM=60.668
PRIMER_LEFT_35_GC_PERCENT=60.000
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=8.1000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=969
PRIMER_PAIR_36_PENALTY=0.923874
PRIMER_LEFT_36_PENALTY=0.510331
PRIMER_RIGHT_36_PENALTY=0.413543
PRIMER_LEFT_36_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_36_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_36=586,20
PRIMER_RIGHT_36=1441,20
PRIMER_LEFT_36_TM=60.510
PRIMER_RIGHT_36_TM=59.586
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.9000
PRIMER_RIGHT_36_END_STABILITY=7.0000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=856
PRIMER_PAIR_37_PENALTY=0.923874
PRIMER_LEFT_37_PENALTY=0.510331
PRIMER_RIGHT_37_PENALTY=0.413543
PRIMER_LEFT_37_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_37_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_37=586,20
PRIMER_RIGHT_37=1442,20
PRIMER_LEFT_37_TM=60.510
PRIMER_RIGHT_37_TM=59.586
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.9000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=857
PRIMER_PAIR_38_PENALTY=0.923874
PRIMER_LEFT_38_PENALTY=0.510331
PRIMER_RIGHT_38_PENALTY=0.413543
PRIMER_LEFT_38_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_38_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_38=586,20
PRIMER_RIGHT_38=1443,20
PRIMER_LEFT_38_TM=60.510
PRIMER_RIGHT_38_TM=59.586
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=40.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.9000
PRIMER_RIGHT_38_END_STABILITY=7.0000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=858
PRIMER_PAIR_39_PENALTY=0.939197
PRIMER_LEFT_39_PENALTY=0.510331
PRIMER_RIGHT_39_PENALTY=0.428866
PRIMER_LEFT_39_SEQUENCE=ACCGTGGAACAGGAGATTCA
PRIMER_RIGHT_39_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_39=586,20
PRIMER_RIGHT_39=1432,20
PRIMER_LEFT_39_TM=60.510
PRIMER_RIGHT_39_TM=59.571
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.9000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=847
PRIMER_PAIR_40_PENALTY=0.991911
PRIMER_LEFT_40_PENALTY=0.578368
PRIMER_RIGHT_40_PENALTY=0.413543
PRIMER_LEFT_40_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_40_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_40=487,20
PRIMER_RIGHT_40=1441,20
PRIMER_LEFT_40_TM=60.578
PRIMER_RIGHT_40_TM=59.586
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=11.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=7.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=955
PRIMER_PAIR_41_PENALTY=0.991911
PRIMER_LEFT_41_PENALTY=0.578368
PRIMER_RIGHT_41_PENALTY=0.413543
PRIMER_LEFT_41_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_41_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_41=487,20
PRIMER_RIGHT_41=1442,20
PRIMER_LEFT_41_TM=60.578
PRIMER_RIGHT_41_TM=59.586
PRIMER_LEFT_41_GC_PERCENT=50.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=11.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=7.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=956
PRIMER_PAIR_42_PENALTY=0.991911
PRIMER_LEFT_42_PENALTY=0.578368
PRIMER_RIGHT_42_PENALTY=0.413543
PRIMER_LEFT_42_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_42_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_42=487,20
PRIMER_RIGHT_42=1443,20
PRIMER_LEFT_42_TM=60.578
PRIMER_RIGHT_42_TM=59.586
PRIMER_LEFT_42_GC_PERCENT=50.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=11.4000
PRIMER_RIGHT_42_END_STABILITY=7.0000
PRIMER_PAIR_42_COMPL_ANY=7.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=957
PRIMER_PAIR_43_PENALTY=1.007234
PRIMER_LEFT_43_PENALTY=0.578368
PRIMER_RIGHT_43_PENALTY=0.428866
PRIMER_LEFT_43_SEQUENCE=GTCCTCCTTCAAATTCGGCT
PRIMER_RIGHT_43_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_43=487,20
PRIMER_RIGHT_43=1432,20
PRIMER_LEFT_43_TM=60.578
PRIMER_RIGHT_43_TM=59.571
PRIMER_LEFT_43_GC_PERCENT=50.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=11.4000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=946
PRIMER_PAIR_44_PENALTY=1.036122
PRIMER_LEFT_44_PENALTY=0.254613
PRIMER_RIGHT_44_PENALTY=0.781508
PRIMER_LEFT_44_SEQUENCE=CGAGGCAGAGCTAGAGGATG
PRIMER_RIGHT_44_SEQUENCE=GTTGCGTTCCCTTTGAGTTT
PRIMER_LEFT_44=677,20
PRIMER_RIGHT_44=1644,20
PRIMER_LEFT_44_TM=60.255
PRIMER_RIGHT_44_TM=59.218
PRIMER_LEFT_44_GC_PERCENT=60.000
PRIMER_RIGHT_44_GC_PERCENT=45.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=8.1000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=968
PRIMER_PAIR_45_PENALTY=1.125334
PRIMER_LEFT_45_PENALTY=0.711791
PRIMER_RIGHT_45_PENALTY=0.413543
PRIMER_LEFT_45_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_45_SEQUENCE=GCCGTATTTTCGGTTTCTTG
PRIMER_LEFT_45=594,20
PRIMER_RIGHT_45=1441,20
PRIMER_LEFT_45_TM=59.288
PRIMER_RIGHT_45_TM=59.586
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=9.6000
PRIMER_RIGHT_45_END_STABILITY=7.0000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=848
PRIMER_PAIR_46_PENALTY=1.125334
PRIMER_LEFT_46_PENALTY=0.711791
PRIMER_RIGHT_46_PENALTY=0.413543
PRIMER_LEFT_46_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_46_SEQUENCE=TGCCGTATTTTCGGTTTCTT
PRIMER_LEFT_46=594,20
PRIMER_RIGHT_46=1442,20
PRIMER_LEFT_46_TM=59.288
PRIMER_RIGHT_46_TM=59.586
PRIMER_LEFT_46_GC_PERCENT=50.000
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=9.6000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=849
PRIMER_PAIR_47_PENALTY=1.125334
PRIMER_LEFT_47_PENALTY=0.711791
PRIMER_RIGHT_47_PENALTY=0.413543
PRIMER_LEFT_47_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_47_SEQUENCE=TTGCCGTATTTTCGGTTTCT
PRIMER_LEFT_47=594,20
PRIMER_RIGHT_47=1443,20
PRIMER_LEFT_47_TM=59.288
PRIMER_RIGHT_47_TM=59.586
PRIMER_LEFT_47_GC_PERCENT=50.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.6000
PRIMER_RIGHT_47_END_STABILITY=7.0000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=850
PRIMER_PAIR_48_PENALTY=1.140657
PRIMER_LEFT_48_PENALTY=0.711791
PRIMER_RIGHT_48_PENALTY=0.428866
PRIMER_LEFT_48_SEQUENCE=ACAGGAGATTCAAAGCGGTC
PRIMER_RIGHT_48_SEQUENCE=TCGGTTTCTTGTGTGTGTCC
PRIMER_LEFT_48=594,20
PRIMER_RIGHT_48=1432,20
PRIMER_LEFT_48_TM=59.288
PRIMER_RIGHT_48_TM=59.571
PRIMER_LEFT_48_GC_PERCENT=50.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=2.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=9.6000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=839
PRIMER_PAIR_49_PENALTY=1.152585
PRIMER_LEFT_49_PENALTY=1.095734
PRIMER_RIGHT_49_PENALTY=0.056850
PRIMER_LEFT_49_SEQUENCE=CAGAGCTAGAGGATGCGGTG
PRIMER_RIGHT_49_SEQUENCE=TTTGCCGTATTTTCGGTTTC
PRIMER_LEFT_49=682,20
PRIMER_RIGHT_49=1444,20
PRIMER_LEFT_49_TM=61.096
PRIMER_RIGHT_49_TM=59.943
PRIMER_LEFT_49_GC_PERCENT=60.000
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=9.9000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=763
=
SEQUENCE_ID=MAGIC.149_5_15592848_15593138_wil-2_wu-0_903881_514271_TRUE
SEQUENCE_TARGET=950,390
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATANNTTNATNAACATAATTTANAATGANTAATATTGNGCNCNANCGATGNCAACCAATGNTNATTGTTTACNANNNNGAATAAATNAAGATATCAAAATNCATAAAATGACTNAAATACCTTAAAGAGAANGTAATTTGTAANTTAATNATNCAAGATTAAGGAACAAGNCAATNNNNNTATAAATTCATANTGTNAGAATCTTTTAGTTTCTCTNTAAGGNATTTGATGCTGGTAATGTTAGTATTCANGATCTCTNTATCACGTTCAAGTTTGGTACAATCCTTATGTGGTTANTAATAGTACATTAAACCTAAACCAAAAATNTAATAATAATGACATGGATTTTTAAGTCAAACCAAAAATCATTAATTTNGGTTTGGTTTGGTAGGGTTTTGAGATANGNTTTTTTTGNCCAAGCCAGGNACTCTATTCCAATNTTNGANANGGGTTTATCCAATCGTGGCATGTTATAACTTATAACTTGAACTAANCCAACAAATTAACATTGACAATTGTTCNANTCAANTTAANAGCANNTGNACATATGCAATTGANTTNTTNTTTTTANTTATCAAAAGATCTCANTTTGCAAATATNCAATTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATTTTNCTNNTTAANNATNAAAAGATACTTTTATTTTAGTGTAGGATATTATTTTCTNANTTGTATNGGNTTTTNNTTCCATCANTTAATTAAAAGCAAANNTTNNNNNATGTATATATNATCTGAAATTGTTNNNTTTTTTTNTNTNNNNATNAAGTGTCACTCAAGATGGAAAAATCTCATTTTTAATATNATNTANTTTGANCTCTTTATATNTCTTATATGTTTCNTACCCTCTTGNTTAAAACTNTTTCTTACATCNAAAAAAATCACGTAACTTAATATGAACATGAAGAATCTCTACCTTGCAATTTTATATCTCTTGGCGGCTTNAACACTTCCATTTGNCATTGCTTCGGACCCAAGCCCCCTTCAAGACTTTTGTATCNGTGTCAACACCCCAGCTAATGCTCGTATGTAAATGTTGATTTTGTANTCCTACTTAGGCTCATCGTTTACTGANNNNNNNNNNNNNNNTATATNTCNNNNAATANTTATNTTANGNTNTATTTGGACANTNTANAATTTANNTTCTNNAGTGTTTGTGAACGGAAAATTCTGCAAGGACCCGAAGCTTNTCACAGCAGATGACTTCTATTTNTNNGGGCTTGACAAGNCAAGAACTACTGAATCTAGTCCAGTTGGGTCTAACGTGACAACCGTGAATGTTAATCAGATTNCAGGGTTAAACACCCTTGGNATCTCACTTGTCNGTATTGATTATGGAATTAATGGACAAAACCCGCCTCACACGCATCCACGCGCTACCGAGNTCTTGCTTGTCCAAGAAGGAANTCTTTTCGTTGGTTTTTTCTCANCATTCCCCGAAAATCGNCTANTCAACAAAACGCTNAACAAAGGGGATGTATTNNTGTTTCCAGAGGGACTCATTCATTTCCAAGTGAACANTGGAAAACAACCCGCTGTTGCATTCGCTTCTCTAAGTAGCCAAAACCCTGGTGTCATCATTATNGGTAACACCTTGTTTGGGTCTAAACCGCCTATAGANCCGAATNTTCNTNCAAAGNCATTTCAGTTGGATCCTAAGGTGATCATTNANCTACANAAANAATTCGGATAAAATGAAAAATTATTNTGGTTTCAATTGCTTTCATATTTTTTCTACCTTAGTCCGTATAATGATCATGCATGTANATATCATCCGTTCATCAATAACGTGACATGATNNTTGGTCAATGTTTTTTCCTTTCATTTGATTGTGATAAAANACCCTNAAANTAGTTGTAATGATCTTTTCTTAGNNATGCCNTAANCAACTATATTNCAATTTCTTNTAATTTTCNACATNGATTAGACTTACATATCTGGTTTNCAGTCGTGACTTGTTTTNTACTGAAGGGACAATNNAACCAAACCAACAAATTCACAATAACGTTTCTTCAACTCAACAGCACATGCNANTGAGNNTTAGGAA
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.777478
PRIMER_LEFT_0_PENALTY=1.543100
PRIMER_RIGHT_0_PENALTY=0.234379
PRIMER_LEFT_0_SEQUENCE=TGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_0_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_0=594,21
PRIMER_RIGHT_0=1417,20
PRIMER_LEFT_0_TM=59.457
PRIMER_RIGHT_0_TM=60.234
PRIMER_LEFT_0_GC_PERCENT=42.857
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=824
PRIMER_PAIR_1_PENALTY=1.858070
PRIMER_LEFT_1_PENALTY=1.543100
PRIMER_RIGHT_1_PENALTY=0.314970
PRIMER_LEFT_1_SEQUENCE=TGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_1_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_1=594,21
PRIMER_RIGHT_1=1420,20
PRIMER_LEFT_1_TM=59.457
PRIMER_RIGHT_1_TM=59.685
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=827
PRIMER_PAIR_2_PENALTY=1.858070
PRIMER_LEFT_2_PENALTY=1.543100
PRIMER_RIGHT_2_PENALTY=0.314970
PRIMER_LEFT_2_SEQUENCE=TGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_2_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_2=594,21
PRIMER_RIGHT_2=1421,20
PRIMER_LEFT_2_TM=59.457
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=828
PRIMER_PAIR_3_PENALTY=2.091974
PRIMER_LEFT_3_PENALTY=1.857595
PRIMER_RIGHT_3_PENALTY=0.234379
PRIMER_LEFT_3_SEQUENCE=GGTTTGGTTTGGTAGGGTTTT
PRIMER_RIGHT_3_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_3=590,21
PRIMER_RIGHT_3=1417,20
PRIMER_LEFT_3_TM=59.142
PRIMER_RIGHT_3_TM=60.234
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=828
PRIMER_PAIR_4_PENALTY=2.172565
PRIMER_LEFT_4_PENALTY=1.857595
PRIMER_RIGHT_4_PENALTY=0.314970
PRIMER_LEFT_4_SEQUENCE=GGTTTGGTTTGGTAGGGTTTT
PRIMER_RIGHT_4_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_4=590,21
PRIMER_RIGHT_4=1420,20
PRIMER_LEFT_4_TM=59.142
PRIMER_RIGHT_4_TM=59.685
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=831
PRIMER_PAIR_5_PENALTY=2.172565
PRIMER_LEFT_5_PENALTY=1.857595
PRIMER_RIGHT_5_PENALTY=0.314970
PRIMER_LEFT_5_SEQUENCE=GGTTTGGTTTGGTAGGGTTTT
PRIMER_RIGHT_5_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_5=590,21
PRIMER_RIGHT_5=1421,20
PRIMER_LEFT_5_TM=59.142
PRIMER_RIGHT_5_TM=59.685
PRIMER_LEFT_5_GC_PERCENT=42.857
PRIMER_RIGHT_5_GC_PERCENT=40.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=9.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PAIR_5_PRODUCT_SIZE=832
PRIMER_PAIR_6_PENALTY=2.253917
PRIMER_LEFT_6_PENALTY=2.019538
PRIMER_RIGHT_6_PENALTY=0.234379
PRIMER_LEFT_6_SEQUENCE=TTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_6_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_6=593,21
PRIMER_RIGHT_6=1417,20
PRIMER_LEFT_6_TM=58.980
PRIMER_RIGHT_6_TM=60.234
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=40.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=825
PRIMER_PAIR_7_PENALTY=2.261450
PRIMER_LEFT_7_PENALTY=2.027071
PRIMER_RIGHT_7_PENALTY=0.234379
PRIMER_LEFT_7_SEQUENCE=TTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_7_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_7=593,20
PRIMER_RIGHT_7=1417,20
PRIMER_LEFT_7_TM=57.973
PRIMER_RIGHT_7_TM=60.234
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=40.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=825
PRIMER_PAIR_8_PENALTY=2.334509
PRIMER_LEFT_8_PENALTY=2.019538
PRIMER_RIGHT_8_PENALTY=0.314970
PRIMER_LEFT_8_SEQUENCE=TTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_8_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_8=593,21
PRIMER_RIGHT_8=1420,20
PRIMER_LEFT_8_TM=58.980
PRIMER_RIGHT_8_TM=59.685
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=45.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=828
PRIMER_PAIR_9_PENALTY=2.334509
PRIMER_LEFT_9_PENALTY=2.019538
PRIMER_RIGHT_9_PENALTY=0.314970
PRIMER_LEFT_9_SEQUENCE=TTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_9_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_9=593,21
PRIMER_RIGHT_9=1421,20
PRIMER_LEFT_9_TM=58.980
PRIMER_RIGHT_9_TM=59.685
PRIMER_LEFT_9_GC_PERCENT=42.857
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=9.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=829
PRIMER_PAIR_10_PENALTY=2.342042
PRIMER_LEFT_10_PENALTY=2.027071
PRIMER_RIGHT_10_PENALTY=0.314970
PRIMER_LEFT_10_SEQUENCE=TTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_10_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_10=593,20
PRIMER_RIGHT_10=1420,20
PRIMER_LEFT_10_TM=57.973
PRIMER_RIGHT_10_TM=59.685
PRIMER_LEFT_10_GC_PERCENT=40.000
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.3000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=828
PRIMER_PAIR_11_PENALTY=2.342042
PRIMER_LEFT_11_PENALTY=2.027071
PRIMER_RIGHT_11_PENALTY=0.314970
PRIMER_LEFT_11_SEQUENCE=TTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_11_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_11=593,20
PRIMER_RIGHT_11=1421,20
PRIMER_LEFT_11_TM=57.973
PRIMER_RIGHT_11_TM=59.685
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=9.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=829
PRIMER_PAIR_12_PENALTY=2.356635
PRIMER_LEFT_12_PENALTY=2.122257
PRIMER_RIGHT_12_PENALTY=0.234379
PRIMER_LEFT_12_SEQUENCE=GTTTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_12_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_12=591,22
PRIMER_RIGHT_12=1417,20
PRIMER_LEFT_12_TM=60.122
PRIMER_RIGHT_12_TM=60.234
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=827
PRIMER_PAIR_13_PENALTY=2.420432
PRIMER_LEFT_13_PENALTY=2.186053
PRIMER_RIGHT_13_PENALTY=0.234379
PRIMER_LEFT_13_SEQUENCE=GGTTTGGTTTGGTAGGGTTT
PRIMER_RIGHT_13_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_13=590,20
PRIMER_RIGHT_13=1417,20
PRIMER_LEFT_13_TM=57.814
PRIMER_RIGHT_13_TM=60.234
PRIMER_LEFT_13_GC_PERCENT=45.000
PRIMER_RIGHT_13_GC_PERCENT=40.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=828
PRIMER_PAIR_14_PENALTY=2.437227
PRIMER_LEFT_14_PENALTY=2.122257
PRIMER_RIGHT_14_PENALTY=0.314970
PRIMER_LEFT_14_SEQUENCE=GTTTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_14_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_14=591,22
PRIMER_RIGHT_14=1420,20
PRIMER_LEFT_14_TM=60.122
PRIMER_RIGHT_14_TM=59.685
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PAIR_14_PRODUCT_SIZE=830
PRIMER_PAIR_15_PENALTY=2.437227
PRIMER_LEFT_15_PENALTY=2.122257
PRIMER_RIGHT_15_PENALTY=0.314970
PRIMER_LEFT_15_SEQUENCE=GTTTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_15_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_15=591,22
PRIMER_RIGHT_15=1421,20
PRIMER_LEFT_15_TM=60.122
PRIMER_RIGHT_15_TM=59.685
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=40.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=831
PRIMER_PAIR_16_PENALTY=2.476392
PRIMER_LEFT_16_PENALTY=2.242013
PRIMER_RIGHT_16_PENALTY=0.234379
PRIMER_LEFT_16_SEQUENCE=TTTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_16_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_16=592,22
PRIMER_RIGHT_16=1417,20
PRIMER_LEFT_16_TM=60.242
PRIMER_RIGHT_16_TM=60.234
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=40.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=826
PRIMER_PAIR_17_PENALTY=2.501024
PRIMER_LEFT_17_PENALTY=2.186053
PRIMER_RIGHT_17_PENALTY=0.314970
PRIMER_LEFT_17_SEQUENCE=GGTTTGGTTTGGTAGGGTTT
PRIMER_RIGHT_17_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_17=590,20
PRIMER_RIGHT_17=1420,20
PRIMER_LEFT_17_TM=57.814
PRIMER_RIGHT_17_TM=59.685
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=831
PRIMER_PAIR_18_PENALTY=2.501024
PRIMER_LEFT_18_PENALTY=2.186053
PRIMER_RIGHT_18_PENALTY=0.314970
PRIMER_LEFT_18_SEQUENCE=GGTTTGGTTTGGTAGGGTTT
PRIMER_RIGHT_18_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_18=590,20
PRIMER_RIGHT_18=1421,20
PRIMER_LEFT_18_TM=57.814
PRIMER_RIGHT_18_TM=59.685
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=9.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=832
PRIMER_PAIR_19_PENALTY=2.556984
PRIMER_LEFT_19_PENALTY=2.242013
PRIMER_RIGHT_19_PENALTY=0.314970
PRIMER_LEFT_19_SEQUENCE=TTTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_19_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_19=592,22
PRIMER_RIGHT_19=1420,20
PRIMER_LEFT_19_TM=60.242
PRIMER_RIGHT_19_TM=59.685
PRIMER_LEFT_19_GC_PERCENT=40.909
PRIMER_RIGHT_19_GC_PERCENT=45.000
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=829
PRIMER_PAIR_20_PENALTY=2.556984
PRIMER_LEFT_20_PENALTY=2.242013
PRIMER_RIGHT_20_PENALTY=0.314970
PRIMER_LEFT_20_SEQUENCE=TTTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_20_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_20=592,22
PRIMER_RIGHT_20=1421,20
PRIMER_LEFT_20_TM=60.242
PRIMER_RIGHT_20_TM=59.685
PRIMER_LEFT_20_GC_PERCENT=40.909
PRIMER_RIGHT_20_GC_PERCENT=40.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=7.0000
PRIMER_RIGHT_20_END_STABILITY=9.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=830
PRIMER_PAIR_21_PENALTY=2.563388
PRIMER_LEFT_21_PENALTY=1.543100
PRIMER_RIGHT_21_PENALTY=1.020289
PRIMER_LEFT_21_SEQUENCE=TGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_21_SEQUENCE=TCACGTTAGACCCAACTGGAC
PRIMER_LEFT_21=594,21
PRIMER_RIGHT_21=1510,21
PRIMER_LEFT_21_TM=59.457
PRIMER_RIGHT_21_TM=60.020
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.7000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=917
PRIMER_PAIR_22_PENALTY=2.651235
PRIMER_LEFT_22_PENALTY=1.543100
PRIMER_RIGHT_22_PENALTY=1.108135
PRIMER_LEFT_22_SEQUENCE=TGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_22_SEQUENCE=CTTGCAGAATTTTCCGTTCA
PRIMER_LEFT_22=594,21
PRIMER_RIGHT_22=1419,20
PRIMER_LEFT_22_TM=59.457
PRIMER_RIGHT_22_TM=58.892
PRIMER_LEFT_22_GC_PERCENT=42.857
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=2.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=2.00
PRIMER_PAIR_22_PRODUCT_SIZE=826
PRIMER_PAIR_23_PENALTY=2.654368
PRIMER_LEFT_23_PENALTY=2.419989
PRIMER_RIGHT_23_PENALTY=0.234379
PRIMER_LEFT_23_SEQUENCE=TGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_23_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_23=594,20
PRIMER_RIGHT_23=1417,20
PRIMER_LEFT_23_TM=57.580
PRIMER_RIGHT_23_TM=60.234
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=40.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.0000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=824
PRIMER_PAIR_24_PENALTY=2.714150
PRIMER_LEFT_24_PENALTY=2.479771
PRIMER_RIGHT_24_PENALTY=0.234379
PRIMER_LEFT_24_SEQUENCE=TTTGGTTTGGTAGGGTTTTG
PRIMER_RIGHT_24_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_24=592,20
PRIMER_RIGHT_24=1417,20
PRIMER_LEFT_24_TM=57.520
PRIMER_RIGHT_24_TM=60.234
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=2.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.6000
PRIMER_RIGHT_24_END_STABILITY=6.7000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=826
PRIMER_PAIR_25_PENALTY=2.734960
PRIMER_LEFT_25_PENALTY=2.419989
PRIMER_RIGHT_25_PENALTY=0.314970
PRIMER_LEFT_25_SEQUENCE=TGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_25_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_25=594,20
PRIMER_RIGHT_25=1420,20
PRIMER_LEFT_25_TM=57.580
PRIMER_RIGHT_25_TM=59.685
PRIMER_LEFT_25_GC_PERCENT=45.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.0000
PRIMER_RIGHT_25_END_STABILITY=8.4000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=827
PRIMER_PAIR_26_PENALTY=2.734960
PRIMER_LEFT_26_PENALTY=2.419989
PRIMER_RIGHT_26_PENALTY=0.314970
PRIMER_LEFT_26_SEQUENCE=TGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_26_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_26=594,20
PRIMER_RIGHT_26=1421,20
PRIMER_LEFT_26_TM=57.580
PRIMER_RIGHT_26_TM=59.685
PRIMER_LEFT_26_GC_PERCENT=45.000
PRIMER_RIGHT_26_GC_PERCENT=40.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.0000
PRIMER_RIGHT_26_END_STABILITY=9.9000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=828
PRIMER_PAIR_27_PENALTY=2.794742
PRIMER_LEFT_27_PENALTY=2.479771
PRIMER_RIGHT_27_PENALTY=0.314970
PRIMER_LEFT_27_SEQUENCE=TTTGGTTTGGTAGGGTTTTG
PRIMER_RIGHT_27_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_27=592,20
PRIMER_RIGHT_27=1420,20
PRIMER_LEFT_27_TM=57.520
PRIMER_RIGHT_27_TM=59.685
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.6000
PRIMER_RIGHT_27_END_STABILITY=8.4000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PAIR_27_PRODUCT_SIZE=829
PRIMER_PAIR_28_PENALTY=2.794742
PRIMER_LEFT_28_PENALTY=2.479771
PRIMER_RIGHT_28_PENALTY=0.314970
PRIMER_LEFT_28_SEQUENCE=TTTGGTTTGGTAGGGTTTTG
PRIMER_RIGHT_28_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_28=592,20
PRIMER_RIGHT_28=1421,20
PRIMER_LEFT_28_TM=57.520
PRIMER_RIGHT_28_TM=59.685
PRIMER_LEFT_28_GC_PERCENT=40.000
PRIMER_RIGHT_28_GC_PERCENT=40.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=7.6000
PRIMER_RIGHT_28_END_STABILITY=9.9000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=830
PRIMER_PAIR_29_PENALTY=2.845175
PRIMER_LEFT_29_PENALTY=2.610796
PRIMER_RIGHT_29_PENALTY=0.234379
PRIMER_LEFT_29_SEQUENCE=GTTTGGTTTGGTAGGGTTTTG
PRIMER_RIGHT_29_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_29=591,21
PRIMER_RIGHT_29=1417,20
PRIMER_LEFT_29_TM=58.389
PRIMER_RIGHT_29_TM=60.234
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.6000
PRIMER_RIGHT_29_END_STABILITY=6.7000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=827
PRIMER_PAIR_30_PENALTY=2.877883
PRIMER_LEFT_30_PENALTY=1.857595
PRIMER_RIGHT_30_PENALTY=1.020289
PRIMER_LEFT_30_SEQUENCE=GGTTTGGTTTGGTAGGGTTTT
PRIMER_RIGHT_30_SEQUENCE=TCACGTTAGACCCAACTGGAC
PRIMER_LEFT_30=590,21
PRIMER_RIGHT_30=1510,21
PRIMER_LEFT_30_TM=59.142
PRIMER_RIGHT_30_TM=60.020
PRIMER_LEFT_30_GC_PERCENT=42.857
PRIMER_RIGHT_30_GC_PERCENT=52.381
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.0000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=921
PRIMER_PAIR_31_PENALTY=2.925767
PRIMER_LEFT_31_PENALTY=2.610796
PRIMER_RIGHT_31_PENALTY=0.314970
PRIMER_LEFT_31_SEQUENCE=GTTTGGTTTGGTAGGGTTTTG
PRIMER_RIGHT_31_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_31=591,21
PRIMER_RIGHT_31=1420,20
PRIMER_LEFT_31_TM=58.389
PRIMER_RIGHT_31_TM=59.685
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=45.000
PRIMER_LEFT_31_SELF_ANY=2.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.6000
PRIMER_RIGHT_31_END_STABILITY=8.4000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=830
PRIMER_PAIR_32_PENALTY=2.925767
PRIMER_LEFT_32_PENALTY=2.610796
PRIMER_RIGHT_32_PENALTY=0.314970
PRIMER_LEFT_32_SEQUENCE=GTTTGGTTTGGTAGGGTTTTG
PRIMER_RIGHT_32_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_32=591,21
PRIMER_RIGHT_32=1421,20
PRIMER_LEFT_32_TM=58.389
PRIMER_RIGHT_32_TM=59.685
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=2.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.6000
PRIMER_RIGHT_32_END_STABILITY=9.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=831
PRIMER_PAIR_33_PENALTY=2.956657
PRIMER_LEFT_33_PENALTY=2.722278
PRIMER_RIGHT_33_PENALTY=0.234379
PRIMER_LEFT_33_SEQUENCE=TTGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_33_SEQUENCE=TGCAGAATTTTCCGTTCACA
PRIMER_LEFT_33=593,22
PRIMER_RIGHT_33=1417,20
PRIMER_LEFT_33_TM=60.722
PRIMER_RIGHT_33_TM=60.234
PRIMER_LEFT_33_GC_PERCENT=40.909
PRIMER_RIGHT_33_GC_PERCENT=40.000
PRIMER_LEFT_33_SELF_ANY=2.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PAIR_33_PRODUCT_SIZE=825
PRIMER_PAIR_34_PENALTY=2.965730
PRIMER_LEFT_34_PENALTY=1.857595
PRIMER_RIGHT_34_PENALTY=1.108135
PRIMER_LEFT_34_SEQUENCE=GGTTTGGTTTGGTAGGGTTTT
PRIMER_RIGHT_34_SEQUENCE=CTTGCAGAATTTTCCGTTCA
PRIMER_LEFT_34=590,21
PRIMER_RIGHT_34=1419,20
PRIMER_LEFT_34_TM=59.142
PRIMER_RIGHT_34_TM=58.892
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=40.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=7.0000
PRIMER_RIGHT_34_END_STABILITY=6.7000
PRIMER_PAIR_34_COMPL_ANY=2.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=830
PRIMER_PAIR_35_PENALTY=3.020125
PRIMER_LEFT_35_PENALTY=1.543100
PRIMER_RIGHT_35_PENALTY=1.477025
PRIMER_LEFT_35_SEQUENCE=TGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_35_SEQUENCE=GTCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_35=594,21
PRIMER_RIGHT_35=1422,21
PRIMER_LEFT_35_TM=59.457
PRIMER_RIGHT_35_TM=60.477
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=42.857
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.7000
PRIMER_RIGHT_35_END_STABILITY=9.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=829
PRIMER_PAIR_36_PENALTY=3.037249
PRIMER_LEFT_36_PENALTY=2.722278
PRIMER_RIGHT_36_PENALTY=0.314970
PRIMER_LEFT_36_SEQUENCE=TTGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_36_SEQUENCE=CCTTGCAGAATTTTCCGTTC
PRIMER_LEFT_36=593,22
PRIMER_RIGHT_36=1420,20
PRIMER_LEFT_36_TM=60.722
PRIMER_RIGHT_36_TM=59.685
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=8.4000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=828
PRIMER_PAIR_37_PENALTY=3.037249
PRIMER_LEFT_37_PENALTY=2.722278
PRIMER_RIGHT_37_PENALTY=0.314970
PRIMER_LEFT_37_SEQUENCE=TTGGTTTGGTAGGGTTTTGAGA
PRIMER_RIGHT_37_SEQUENCE=TCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_37=593,22
PRIMER_RIGHT_37=1421,20
PRIMER_LEFT_37_TM=60.722
PRIMER_RIGHT_37_TM=59.685
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.7000
PRIMER_RIGHT_37_END_STABILITY=9.9000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=829
PRIMER_PAIR_38_PENALTY=3.039827
PRIMER_LEFT_38_PENALTY=2.019538
PRIMER_RIGHT_38_PENALTY=1.020289
PRIMER_LEFT_38_SEQUENCE=TTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_38_SEQUENCE=TCACGTTAGACCCAACTGGAC
PRIMER_LEFT_38=593,21
PRIMER_RIGHT_38=1510,21
PRIMER_LEFT_38_TM=58.980
PRIMER_RIGHT_38_TM=60.020
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=52.381
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.0000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=918
PRIMER_PAIR_39_PENALTY=3.047360
PRIMER_LEFT_39_PENALTY=2.027071
PRIMER_RIGHT_39_PENALTY=1.020289
PRIMER_LEFT_39_SEQUENCE=TTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_39_SEQUENCE=TCACGTTAGACCCAACTGGAC
PRIMER_LEFT_39=593,20
PRIMER_RIGHT_39=1510,21
PRIMER_LEFT_39_TM=57.973
PRIMER_RIGHT_39_TM=60.020
PRIMER_LEFT_39_GC_PERCENT=40.000
PRIMER_RIGHT_39_GC_PERCENT=52.381
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=918
PRIMER_PAIR_40_PENALTY=3.110341
PRIMER_LEFT_40_PENALTY=1.857595
PRIMER_RIGHT_40_PENALTY=1.252746
PRIMER_LEFT_40_SEQUENCE=GGTTTGGTTTGGTAGGGTTTT
PRIMER_RIGHT_40_SEQUENCE=GCAGAATTTTCCGTTCACAA
PRIMER_LEFT_40=590,21
PRIMER_RIGHT_40=1416,20
PRIMER_LEFT_40_TM=59.142
PRIMER_RIGHT_40_TM=58.747
PRIMER_LEFT_40_GC_PERCENT=42.857
PRIMER_RIGHT_40_GC_PERCENT=40.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=4.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.0000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=827
PRIMER_PAIR_41_PENALTY=3.127673
PRIMER_LEFT_41_PENALTY=2.019538
PRIMER_RIGHT_41_PENALTY=1.108135
PRIMER_LEFT_41_SEQUENCE=TTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_41_SEQUENCE=CTTGCAGAATTTTCCGTTCA
PRIMER_LEFT_41=593,21
PRIMER_RIGHT_41=1419,20
PRIMER_LEFT_41_TM=58.980
PRIMER_RIGHT_41_TM=58.892
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=7.0000
PRIMER_RIGHT_41_END_STABILITY=6.7000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=2.00
PRIMER_PAIR_41_PRODUCT_SIZE=827
PRIMER_PAIR_42_PENALTY=3.135207
PRIMER_LEFT_42_PENALTY=2.027071
PRIMER_RIGHT_42_PENALTY=1.108135
PRIMER_LEFT_42_SEQUENCE=TTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_42_SEQUENCE=CTTGCAGAATTTTCCGTTCA
PRIMER_LEFT_42=593,20
PRIMER_RIGHT_42=1419,20
PRIMER_LEFT_42_TM=57.973
PRIMER_RIGHT_42_TM=58.892
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=40.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=7.3000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=827
PRIMER_PAIR_43_PENALTY=3.142545
PRIMER_LEFT_43_PENALTY=2.122257
PRIMER_RIGHT_43_PENALTY=1.020289
PRIMER_LEFT_43_SEQUENCE=GTTTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_43_SEQUENCE=TCACGTTAGACCCAACTGGAC
PRIMER_LEFT_43=591,22
PRIMER_RIGHT_43=1510,21
PRIMER_LEFT_43_TM=60.122
PRIMER_RIGHT_43_TM=60.020
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=7.3000
PRIMER_RIGHT_43_END_STABILITY=7.9000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=920
PRIMER_PAIR_44_PENALTY=3.230392
PRIMER_LEFT_44_PENALTY=2.122257
PRIMER_RIGHT_44_PENALTY=1.108135
PRIMER_LEFT_44_SEQUENCE=GTTTGGTTTGGTAGGGTTTTGA
PRIMER_RIGHT_44_SEQUENCE=CTTGCAGAATTTTCCGTTCA
PRIMER_LEFT_44=591,22
PRIMER_RIGHT_44=1419,20
PRIMER_LEFT_44_TM=60.122
PRIMER_RIGHT_44_TM=58.892
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=40.000
PRIMER_LEFT_44_SELF_ANY=2.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=7.3000
PRIMER_RIGHT_44_END_STABILITY=6.7000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=3.00
PRIMER_PAIR_44_PRODUCT_SIZE=829
PRIMER_PAIR_45_PENALTY=3.262302
PRIMER_LEFT_45_PENALTY=2.242013
PRIMER_RIGHT_45_PENALTY=1.020289
PRIMER_LEFT_45_SEQUENCE=TTTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_45_SEQUENCE=TCACGTTAGACCCAACTGGAC
PRIMER_LEFT_45=592,22
PRIMER_RIGHT_45=1510,21
PRIMER_LEFT_45_TM=60.242
PRIMER_RIGHT_45_TM=60.020
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=52.381
PRIMER_LEFT_45_SELF_ANY=2.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=7.0000
PRIMER_RIGHT_45_END_STABILITY=7.9000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=2.00
PRIMER_PAIR_45_PRODUCT_SIZE=919
PRIMER_PAIR_46_PENALTY=3.272285
PRIMER_LEFT_46_PENALTY=2.019538
PRIMER_RIGHT_46_PENALTY=1.252746
PRIMER_LEFT_46_SEQUENCE=TTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_46_SEQUENCE=GCAGAATTTTCCGTTCACAA
PRIMER_LEFT_46=593,21
PRIMER_RIGHT_46=1416,20
PRIMER_LEFT_46_TM=58.980
PRIMER_RIGHT_46_TM=58.747
PRIMER_LEFT_46_GC_PERCENT=42.857
PRIMER_RIGHT_46_GC_PERCENT=40.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.0000
PRIMER_RIGHT_46_END_STABILITY=7.0000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=824
PRIMER_PAIR_47_PENALTY=3.294189
PRIMER_LEFT_47_PENALTY=2.186053
PRIMER_RIGHT_47_PENALTY=1.108135
PRIMER_LEFT_47_SEQUENCE=GGTTTGGTTTGGTAGGGTTT
PRIMER_RIGHT_47_SEQUENCE=CTTGCAGAATTTTCCGTTCA
PRIMER_LEFT_47=590,20
PRIMER_RIGHT_47=1419,20
PRIMER_LEFT_47_TM=57.814
PRIMER_RIGHT_47_TM=58.892
PRIMER_LEFT_47_GC_PERCENT=45.000
PRIMER_RIGHT_47_GC_PERCENT=40.000
PRIMER_LEFT_47_SELF_ANY=2.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=8.2000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=2.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=830
PRIMER_PAIR_48_PENALTY=3.334620
PRIMER_LEFT_48_PENALTY=1.857595
PRIMER_RIGHT_48_PENALTY=1.477025
PRIMER_LEFT_48_SEQUENCE=GGTTTGGTTTGGTAGGGTTTT
PRIMER_RIGHT_48_SEQUENCE=GTCCTTGCAGAATTTTCCGTT
PRIMER_LEFT_48=590,21
PRIMER_RIGHT_48=1422,21
PRIMER_LEFT_48_TM=59.142
PRIMER_RIGHT_48_TM=60.477
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=9.9000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=833
PRIMER_PAIR_49_PENALTY=3.350148
PRIMER_LEFT_49_PENALTY=2.242013
PRIMER_RIGHT_49_PENALTY=1.108135
PRIMER_LEFT_49_SEQUENCE=TTTGGTTTGGTAGGGTTTTGAG
PRIMER_RIGHT_49_SEQUENCE=CTTGCAGAATTTTCCGTTCA
PRIMER_LEFT_49=592,22
PRIMER_RIGHT_49=1419,20
PRIMER_LEFT_49_TM=60.242
PRIMER_RIGHT_49_TM=58.892
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=2.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=7.0000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=828
=
SEQUENCE_ID=MAGIC.338_5_14777962_14777979_tsu-0_po-0_5259_119060_TRUE
SEQUENCE_TARGET=950,117
SEQUENCE_TEMPLATE=CCATAGATGNCACGTTATTTTTACCNGTTTTATANTNCATNNCAATTTATGACTAAACCCATTAGTTTGTTTNAACTTNTATCAAATGCTAGATAATTTACCCGTGCGTATTATATACATCTCTTGTTGATAATATTTNNTANGTATTATACANTTGTGTTTGATTTGATCNAGAAGCAANNNNNNAAACATNATTGAAATTTTTGTGTGGATATCNNNNNNNNNNAAAATGGAGNACATTTTTGAATGANNGTTCCAATTTGGCAAGTTGGAGATCGTATGTGCATATATTTTTCTCCTCTAGAGACGTTTGGAACAACATTTGGCATGTCAAACCNCTTGTGTCATGGTATCCTTTGGNTNGGCACANNNNNNNAAGGCTGCGATTCCAAAACACTCTTTTCACGTTGGACAGAAAAGATTGTTCCGAGACGTTGTCCNCATAAAAGACAATANATAATGTGTTTTATTTNATAAATTAAGCGGCNNNNAAGTAAACGANGTAAAAGCAATAAGAGNAANGTATTATGCANTAGGGTTNTTCGTANNCATGAATTGTATAANCNTGTANAAAATCATNNNNNNNTTGGNTATTTTNNNTTTNNNCAATCCAAACNTNTGNNNTTTTTNNAAACCTTTATNGAGTAATCTCTTTNGTTTGCTTCATCTCCCAATTNAATAAAAGTCATTGGGAAAGCACNNAGTNTCGCGTGGTTTGATGTTTTGAATANTCACGTTNANTTAAAATAAGATTATACACGAAANCTCTTCGTATTCCCTANNNNATATATAGTCCATANGCATATTANNTANTGGNTATATCCTCGAGACTTGTANCTCTAATTATCCATCTACANGGAAGCTCAAAAGNTTAANTCTTTATGTACCACTNNGATTTTTGTGGAGTTTGTTTTTTANCCTGCACTCATTTTCTCTCATCTTTCGTGAANTATTTCTCCAGAATCTCTAATGGATCAANGATCAGNGAAAANNATGATCANTGTTCTTCTTNTACATNATGNGGACAAGAAAAANGTTGTCTGGATTCAATCTGGTANTGACTTNGTTGATTTGTTNTNTAGTTTTCTTACCATGCCTTNGGGANCAATTGTGAGATTGCTTGAGCATCACGGAAGATTAAAAGAAAANGTCAGCATAGGTTGTTTCAACAACCNTTATAAAAGTCTTGTAGACATGAGCATCGATAATTTCAGGAACGAAGCTTGTAAGCAGATGNTGGTTTACNCNNNNNNNNNGAAGGAACGTCAATGCAGAATGCTGAAGATTAACATAGACGACACAGAAGNAACCAAGTNCTTTATGTGTCCAANGTNTTTGCAAAGCNCATCATGCAGAGAGTTNTTTAGTAACTTCAATACTTCAAGATGTAGGTGTGGAAACTTGATGAACAAAGAGANTTCNTCACCANAANGAGAAGAATCTGTAAGCAGATTGGGANGTAGTTGTGNTGATGATGGAGTCTTTGTCCATGGTGATGGAAATATGGCATTTNTGTTGAGTGATGATTTGACAATCGAGAATTTTTCTTGGNATCTTCNCCGGAAACGTGTCAAGGATTTAGGTTGCGTTAACGTNTTCGATAAGTTAGGGGAGGGTGAANCAGAANTTGGCTTCCGNNAGGTANTTTTATNGTNTTGTTCTCTTANTTTTTTTTTTGGTTTANTAGAANNAAACTTTNTGNCTTGTGCANTCTCANTGTATAGGCAATNACTTTGCTACGATCNTTATTCACATCAGANANTCCATTGACAGCNACATTCNNCCCCTTTCATAGTTNGCCATATCCTTCTAAGAGAGCATATNAGNCATCGAGTCCATGNNATTCGNTTTTAGGCCNNNNNNNNNNNNNNNAAGTGTATTTGAGTAAGGNGGACAACAAGAAGGTTATTTACGCTGAATGTGGTGAANGCTTTATTGATCTTCTTTGNANGTTTCTTGNTCTTCCTCTTGNGTATANATGTNAGATCTCTAGTANNNNNNNNNNTGATGATGATGG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=1.151594
PRIMER_LEFT_0_PENALTY=0.026532
PRIMER_RIGHT_0_PENALTY=1.125062
PRIMER_LEFT_0_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_0_SEQUENCE=CATTCTGCATTGACGTTCCTT
PRIMER_LEFT_0=420,20
PRIMER_RIGHT_0=1277,21
PRIMER_LEFT_0_TM=59.973
PRIMER_RIGHT_0_TM=60.125
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=858
PRIMER_PAIR_1_PENALTY=1.176431
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=1.149900
PRIMER_LEFT_1_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_1_SEQUENCE=TTCTGCATTGACGTTCCTTC
PRIMER_LEFT_1=420,20
PRIMER_RIGHT_1=1275,20
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=58.850
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=856
PRIMER_PAIR_2_PENALTY=1.302542
PRIMER_LEFT_2_PENALTY=0.026532
PRIMER_RIGHT_2_PENALTY=1.276010
PRIMER_LEFT_2_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_2_SEQUENCE=CATTCTGCATTGACGTTCCT
PRIMER_LEFT_2=420,20
PRIMER_RIGHT_2=1277,20
PRIMER_LEFT_2_TM=59.973
PRIMER_RIGHT_2_TM=58.724
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=858
PRIMER_PAIR_3_PENALTY=1.380746
PRIMER_LEFT_3_PENALTY=0.026532
PRIMER_RIGHT_3_PENALTY=1.354214
PRIMER_LEFT_3_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_3_SEQUENCE=TCTGCTTACAAGCTTCGTTCC
PRIMER_LEFT_3=420,20
PRIMER_RIGHT_3=1233,21
PRIMER_LEFT_3_TM=59.973
PRIMER_RIGHT_3_TM=59.646
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=814
PRIMER_PAIR_4_PENALTY=1.582019
PRIMER_LEFT_4_PENALTY=0.026532
PRIMER_RIGHT_4_PENALTY=1.555487
PRIMER_LEFT_4_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_4_SEQUENCE=TGATGCTCAAGCAATCTCACA
PRIMER_LEFT_4=420,20
PRIMER_RIGHT_4=1129,21
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=60.555
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=710
PRIMER_PAIR_5_PENALTY=1.586932
PRIMER_LEFT_5_PENALTY=0.026532
PRIMER_RIGHT_5_PENALTY=1.560400
PRIMER_LEFT_5_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_5_SEQUENCE=TGCTTACAAGCTTCGTTCCTG
PRIMER_LEFT_5=420,20
PRIMER_RIGHT_5=1231,21
PRIMER_LEFT_5_TM=59.973
PRIMER_RIGHT_5_TM=60.560
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=812
PRIMER_PAIR_6_PENALTY=1.628134
PRIMER_LEFT_6_PENALTY=0.026532
PRIMER_RIGHT_6_PENALTY=1.601602
PRIMER_LEFT_6_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_6_SEQUENCE=GCATTCTGCATTGACGTTCC
PRIMER_LEFT_6=420,20
PRIMER_RIGHT_6=1278,20
PRIMER_LEFT_6_TM=59.973
PRIMER_RIGHT_6_TM=61.602
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=859
PRIMER_PAIR_7_PENALTY=1.838317
PRIMER_LEFT_7_PENALTY=0.026532
PRIMER_RIGHT_7_PENALTY=1.811785
PRIMER_LEFT_7_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_7_SEQUENCE=ATTCTGCATTGACGTTCCTTC
PRIMER_LEFT_7=420,20
PRIMER_RIGHT_7=1276,21
PRIMER_LEFT_7_TM=59.973
PRIMER_RIGHT_7_TM=59.188
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=42.857
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=857
PRIMER_PAIR_8_PENALTY=1.910985
PRIMER_LEFT_8_PENALTY=0.785923
PRIMER_RIGHT_8_PENALTY=1.125062
PRIMER_LEFT_8_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_8_SEQUENCE=CATTCTGCATTGACGTTCCTT
PRIMER_LEFT_8=379,20
PRIMER_RIGHT_8=1277,21
PRIMER_LEFT_8_TM=60.786
PRIMER_RIGHT_8_TM=60.125
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=899
PRIMER_PAIR_9_PENALTY=1.935823
PRIMER_LEFT_9_PENALTY=0.785923
PRIMER_RIGHT_9_PENALTY=1.149900
PRIMER_LEFT_9_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_9_SEQUENCE=TTCTGCATTGACGTTCCTTC
PRIMER_LEFT_9=379,20
PRIMER_RIGHT_9=1275,20
PRIMER_LEFT_9_TM=60.786
PRIMER_RIGHT_9_TM=58.850
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=897
PRIMER_PAIR_10_PENALTY=2.010057
PRIMER_LEFT_10_PENALTY=0.026532
PRIMER_RIGHT_10_PENALTY=1.983525
PRIMER_LEFT_10_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_10_SEQUENCE=GATGCTCAAGCAATCTCACAA
PRIMER_LEFT_10=420,20
PRIMER_RIGHT_10=1128,21
PRIMER_LEFT_10_TM=59.973
PRIMER_RIGHT_10_TM=59.016
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=42.857
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=709
PRIMER_PAIR_11_PENALTY=2.010057
PRIMER_LEFT_11_PENALTY=0.026532
PRIMER_RIGHT_11_PENALTY=1.983525
PRIMER_LEFT_11_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_11_SEQUENCE=TCTTCAGCATTCTGCATTGAC
PRIMER_LEFT_11=420,20
PRIMER_RIGHT_11=1284,21
PRIMER_LEFT_11_TM=59.973
PRIMER_RIGHT_11_TM=59.016
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=42.857
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=865
PRIMER_PAIR_12_PENALTY=2.061933
PRIMER_LEFT_12_PENALTY=0.785923
PRIMER_RIGHT_12_PENALTY=1.276010
PRIMER_LEFT_12_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_12_SEQUENCE=CATTCTGCATTGACGTTCCT
PRIMER_LEFT_12=379,20
PRIMER_RIGHT_12=1277,20
PRIMER_LEFT_12_TM=60.786
PRIMER_RIGHT_12_TM=58.724
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=45.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=899
PRIMER_PAIR_13_PENALTY=2.107419
PRIMER_LEFT_13_PENALTY=0.026532
PRIMER_RIGHT_13_PENALTY=2.080887
PRIMER_LEFT_13_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_13_SEQUENCE=ATCTGCTTACAAGCTTCGTTCC
PRIMER_LEFT_13=420,20
PRIMER_RIGHT_13=1234,22
PRIMER_LEFT_13_TM=59.973
PRIMER_RIGHT_13_TM=59.919
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=815
PRIMER_PAIR_14_PENALTY=2.140137
PRIMER_LEFT_14_PENALTY=0.785923
PRIMER_RIGHT_14_PENALTY=1.354214
PRIMER_LEFT_14_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_14_SEQUENCE=TCTGCTTACAAGCTTCGTTCC
PRIMER_LEFT_14=379,20
PRIMER_RIGHT_14=1233,21
PRIMER_LEFT_14_TM=60.786
PRIMER_RIGHT_14_TM=59.646
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=47.619
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=855
PRIMER_PAIR_15_PENALTY=2.151187
PRIMER_LEFT_15_PENALTY=0.026532
PRIMER_RIGHT_15_PENALTY=2.124655
PRIMER_LEFT_15_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_15_SEQUENCE=TTGTTCATCAAGTTTCCACACC
PRIMER_LEFT_15=420,20
PRIMER_RIGHT_15=1411,22
PRIMER_LEFT_15_TM=59.973
PRIMER_RIGHT_15_TM=59.875
PRIMER_LEFT_15_GC_PERCENT=50.000
PRIMER_RIGHT_15_GC_PERCENT=40.909
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=992
PRIMER_PAIR_16_PENALTY=2.223924
PRIMER_LEFT_16_PENALTY=0.026532
PRIMER_RIGHT_16_PENALTY=2.197392
PRIMER_LEFT_16_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_16_SEQUENCE=CTGCTTACAAGCTTCGTTCCT
PRIMER_LEFT_16=420,20
PRIMER_RIGHT_16=1232,21
PRIMER_LEFT_16_TM=59.973
PRIMER_RIGHT_16_TM=58.803
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=813
PRIMER_PAIR_17_PENALTY=2.241967
PRIMER_LEFT_17_PENALTY=0.026532
PRIMER_RIGHT_17_PENALTY=2.215435
PRIMER_LEFT_17_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_17_SEQUENCE=GCTTACAAGCTTCGTTCCTG
PRIMER_LEFT_17=420,20
PRIMER_RIGHT_17=1230,20
PRIMER_LEFT_17_TM=59.973
PRIMER_RIGHT_17_TM=57.785
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=811
PRIMER_PAIR_18_PENALTY=2.241967
PRIMER_LEFT_18_PENALTY=0.026532
PRIMER_RIGHT_18_PENALTY=2.215435
PRIMER_LEFT_18_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_18_SEQUENCE=TGCTTACAAGCTTCGTTCCT
PRIMER_LEFT_18=420,20
PRIMER_RIGHT_18=1231,20
PRIMER_LEFT_18_TM=59.973
PRIMER_RIGHT_18_TM=57.785
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=45.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=812
PRIMER_PAIR_19_PENALTY=2.241967
PRIMER_LEFT_19_PENALTY=0.026532
PRIMER_RIGHT_19_PENALTY=2.215435
PRIMER_LEFT_19_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_19_SEQUENCE=CTGCTTACAAGCTTCGTTCC
PRIMER_LEFT_19=420,20
PRIMER_RIGHT_19=1232,20
PRIMER_LEFT_19_TM=59.973
PRIMER_RIGHT_19_TM=57.785
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=813
PRIMER_PAIR_20_PENALTY=2.341410
PRIMER_LEFT_20_PENALTY=0.785923
PRIMER_RIGHT_20_PENALTY=1.555487
PRIMER_LEFT_20_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_20_SEQUENCE=TGATGCTCAAGCAATCTCACA
PRIMER_LEFT_20=379,20
PRIMER_RIGHT_20=1129,21
PRIMER_LEFT_20_TM=60.786
PRIMER_RIGHT_20_TM=60.555
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=42.857
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.7000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=751
PRIMER_PAIR_21_PENALTY=2.346323
PRIMER_LEFT_21_PENALTY=0.785923
PRIMER_RIGHT_21_PENALTY=1.560400
PRIMER_LEFT_21_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_21_SEQUENCE=TGCTTACAAGCTTCGTTCCTG
PRIMER_LEFT_21=379,20
PRIMER_RIGHT_21=1231,21
PRIMER_LEFT_21_TM=60.786
PRIMER_RIGHT_21_TM=60.560
PRIMER_LEFT_21_GC_PERCENT=50.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=3.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=853
PRIMER_PAIR_22_PENALTY=2.387525
PRIMER_LEFT_22_PENALTY=0.785923
PRIMER_RIGHT_22_PENALTY=1.601602
PRIMER_LEFT_22_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_22_SEQUENCE=GCATTCTGCATTGACGTTCC
PRIMER_LEFT_22=379,20
PRIMER_RIGHT_22=1278,20
PRIMER_LEFT_22_TM=60.786
PRIMER_RIGHT_22_TM=61.602
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=900
PRIMER_PAIR_23_PENALTY=2.556047
PRIMER_LEFT_23_PENALTY=0.026532
PRIMER_RIGHT_23_PENALTY=2.529515
PRIMER_LEFT_23_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_23_SEQUENCE=GATGCTCAAGCAATCTCACA
PRIMER_LEFT_23=420,20
PRIMER_RIGHT_23=1128,20
PRIMER_LEFT_23_TM=59.973
PRIMER_RIGHT_23_TM=57.470
PRIMER_LEFT_23_GC_PERCENT=50.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=709
PRIMER_PAIR_24_PENALTY=2.556047
PRIMER_LEFT_24_PENALTY=0.026532
PRIMER_RIGHT_24_PENALTY=2.529515
PRIMER_LEFT_24_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_24_SEQUENCE=TGATGCTCAAGCAATCTCAC
PRIMER_LEFT_24=420,20
PRIMER_RIGHT_24=1129,20
PRIMER_LEFT_24_TM=59.973
PRIMER_RIGHT_24_TM=57.470
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PAIR_24_PRODUCT_SIZE=710
PRIMER_PAIR_25_PENALTY=2.570412
PRIMER_LEFT_25_PENALTY=0.026532
PRIMER_RIGHT_25_PENALTY=2.543880
PRIMER_LEFT_25_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_25_SEQUENCE=TGTTCATCAAGTTTCCACACC
PRIMER_LEFT_25=420,20
PRIMER_RIGHT_25=1410,21
PRIMER_LEFT_25_TM=59.973
PRIMER_RIGHT_25_TM=58.456
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=42.857
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=7.6000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=991
PRIMER_PAIR_26_PENALTY=2.579355
PRIMER_LEFT_26_PENALTY=0.026532
PRIMER_RIGHT_26_PENALTY=2.552823
PRIMER_LEFT_26_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_26_SEQUENCE=TCTGCTTACAAGCTTCGTTCCT
PRIMER_LEFT_26=420,20
PRIMER_RIGHT_26=1233,22
PRIMER_LEFT_26_TM=59.973
PRIMER_RIGHT_26_TM=60.553
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=45.455
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=814
PRIMER_PAIR_27_PENALTY=2.597708
PRIMER_LEFT_27_PENALTY=0.785923
PRIMER_RIGHT_27_PENALTY=1.811785
PRIMER_LEFT_27_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_27_SEQUENCE=ATTCTGCATTGACGTTCCTTC
PRIMER_LEFT_27=379,20
PRIMER_RIGHT_27=1276,21
PRIMER_LEFT_27_TM=60.786
PRIMER_RIGHT_27_TM=59.188
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=42.857
PRIMER_LEFT_27_SELF_ANY=3.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=898
PRIMER_PAIR_28_PENALTY=2.682080
PRIMER_LEFT_28_PENALTY=0.026532
PRIMER_RIGHT_28_PENALTY=2.655548
PRIMER_LEFT_28_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_28_SEQUENCE=ATCTTCAGCATTCTGCATTGAC
PRIMER_LEFT_28=420,20
PRIMER_RIGHT_28=1285,22
PRIMER_LEFT_28_TM=59.973
PRIMER_RIGHT_28_TM=59.344
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=40.909
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=7.9000
PRIMER_RIGHT_28_END_STABILITY=6.7000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=866
PRIMER_PAIR_29_PENALTY=2.761914
PRIMER_LEFT_29_PENALTY=0.026532
PRIMER_RIGHT_29_PENALTY=2.735382
PRIMER_LEFT_29_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_29_SEQUENCE=ATTCTGCATTGACGTTCCTT
PRIMER_LEFT_29=420,20
PRIMER_RIGHT_29=1276,20
PRIMER_LEFT_29_TM=59.973
PRIMER_RIGHT_29_TM=57.265
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=7.9000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=857
PRIMER_PAIR_30_PENALTY=2.769448
PRIMER_LEFT_30_PENALTY=0.785923
PRIMER_RIGHT_30_PENALTY=1.983525
PRIMER_LEFT_30_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_30_SEQUENCE=GATGCTCAAGCAATCTCACAA
PRIMER_LEFT_30=379,20
PRIMER_RIGHT_30=1128,21
PRIMER_LEFT_30_TM=60.786
PRIMER_RIGHT_30_TM=59.016
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=42.857
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.0000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=750
PRIMER_PAIR_31_PENALTY=2.769448
PRIMER_LEFT_31_PENALTY=0.785923
PRIMER_RIGHT_31_PENALTY=1.983525
PRIMER_LEFT_31_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_31_SEQUENCE=TCTTCAGCATTCTGCATTGAC
PRIMER_LEFT_31=379,20
PRIMER_RIGHT_31=1284,21
PRIMER_LEFT_31_TM=60.786
PRIMER_RIGHT_31_TM=59.016
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=42.857
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=906
PRIMER_PAIR_32_PENALTY=2.866810
PRIMER_LEFT_32_PENALTY=0.785923
PRIMER_RIGHT_32_PENALTY=2.080887
PRIMER_LEFT_32_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_32_SEQUENCE=ATCTGCTTACAAGCTTCGTTCC
PRIMER_LEFT_32=379,20
PRIMER_RIGHT_32=1234,22
PRIMER_LEFT_32_TM=60.786
PRIMER_RIGHT_32_TM=59.919
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=45.455
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=856
PRIMER_PAIR_33_PENALTY=2.896605
PRIMER_LEFT_33_PENALTY=1.771543
PRIMER_RIGHT_33_PENALTY=1.125062
PRIMER_LEFT_33_SEQUENCE=AAAGATTGTTCCGAGACGTTG
PRIMER_RIGHT_33_SEQUENCE=CATTCTGCATTGACGTTCCTT
PRIMER_LEFT_33=416,21
PRIMER_RIGHT_33=1277,21
PRIMER_LEFT_33_TM=59.228
PRIMER_RIGHT_33_TM=60.125
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=42.857
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.7000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=862
PRIMER_PAIR_34_PENALTY=2.921443
PRIMER_LEFT_34_PENALTY=1.771543
PRIMER_RIGHT_34_PENALTY=1.149900
PRIMER_LEFT_34_SEQUENCE=AAAGATTGTTCCGAGACGTTG
PRIMER_RIGHT_34_SEQUENCE=TTCTGCATTGACGTTCCTTC
PRIMER_LEFT_34=416,21
PRIMER_RIGHT_34=1275,20
PRIMER_LEFT_34_TM=59.228
PRIMER_RIGHT_34_TM=58.850
PRIMER_LEFT_34_GC_PERCENT=42.857
PRIMER_RIGHT_34_GC_PERCENT=45.000
PRIMER_LEFT_34_SELF_ANY=4.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=860
PRIMER_PAIR_35_PENALTY=2.983315
PRIMER_LEFT_35_PENALTY=0.785923
PRIMER_RIGHT_35_PENALTY=2.197392
PRIMER_LEFT_35_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_35_SEQUENCE=CTGCTTACAAGCTTCGTTCCT
PRIMER_LEFT_35=379,20
PRIMER_RIGHT_35=1232,21
PRIMER_LEFT_35_TM=60.786
PRIMER_RIGHT_35_TM=58.803
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=47.619
PRIMER_LEFT_35_SELF_ANY=3.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=854
PRIMER_PAIR_36_PENALTY=3.001358
PRIMER_LEFT_36_PENALTY=0.785923
PRIMER_RIGHT_36_PENALTY=2.215435
PRIMER_LEFT_36_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_36_SEQUENCE=GCTTACAAGCTTCGTTCCTG
PRIMER_LEFT_36=379,20
PRIMER_RIGHT_36=1230,20
PRIMER_LEFT_36_TM=60.786
PRIMER_RIGHT_36_TM=57.785
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=852
PRIMER_PAIR_37_PENALTY=3.001358
PRIMER_LEFT_37_PENALTY=0.785923
PRIMER_RIGHT_37_PENALTY=2.215435
PRIMER_LEFT_37_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_37_SEQUENCE=TGCTTACAAGCTTCGTTCCT
PRIMER_LEFT_37=379,20
PRIMER_RIGHT_37=1231,20
PRIMER_LEFT_37_TM=60.786
PRIMER_RIGHT_37_TM=57.785
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=3.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=853
PRIMER_PAIR_38_PENALTY=3.001358
PRIMER_LEFT_38_PENALTY=0.785923
PRIMER_RIGHT_38_PENALTY=2.215435
PRIMER_LEFT_38_SEQUENCE=GCTGCGATTCCAAAACACTC
PRIMER_RIGHT_38_SEQUENCE=CTGCTTACAAGCTTCGTTCC
PRIMER_LEFT_38=379,20
PRIMER_RIGHT_38=1232,20
PRIMER_LEFT_38_TM=60.786
PRIMER_RIGHT_38_TM=57.785
PRIMER_LEFT_38_GC_PERCENT=50.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=7.9000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=854
PRIMER_PAIR_39_PENALTY=3.045809
PRIMER_LEFT_39_PENALTY=0.026532
PRIMER_RIGHT_39_PENALTY=3.019278
PRIMER_LEFT_39_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_39_SEQUENCE=ATGCTCAAGCAATCTCACAA
PRIMER_LEFT_39=420,20
PRIMER_RIGHT_39=1127,20
PRIMER_LEFT_39_TM=59.973
PRIMER_RIGHT_39_TM=56.981
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=7.0000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=708
PRIMER_PAIR_40_PENALTY=3.045809
PRIMER_LEFT_40_PENALTY=0.026532
PRIMER_RIGHT_40_PENALTY=3.019278
PRIMER_LEFT_40_SEQUENCE=ATTGTTCCGAGACGTTGTCC
PRIMER_RIGHT_40_SEQUENCE=CTTCAGCATTCTGCATTGAC
PRIMER_LEFT_40=420,20
PRIMER_RIGHT_40=1283,20
PRIMER_LEFT_40_TM=59.973
PRIMER_RIGHT_40_TM=56.981
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=6.7000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=2.00
PRIMER_PAIR_40_PRODUCT_SIZE=864
PRIMER_PAIR_41_PENALTY=3.047553
PRIMER_LEFT_41_PENALTY=1.771543
PRIMER_RIGHT_41_PENALTY=1.276010
PRIMER_LEFT_41_SEQUENCE=AAAGATTGTTCCGAGACGTTG
PRIMER_RIGHT_41_SEQUENCE=CATTCTGCATTGACGTTCCT
PRIMER_LEFT_41=416,21
PRIMER_RIGHT_41=1277,20
PRIMER_LEFT_41_TM=59.228
PRIMER_RIGHT_41_TM=58.724
PRIMER_LEFT_41_GC_PERCENT=42.857
PRIMER_RIGHT_41_GC_PERCENT=45.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=8.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=4.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=862
PRIMER_PAIR_42_PENALTY=3.125757
PRIMER_LEFT_42_PENALTY=1.771543
PRIMER_RIGHT_42_PENALTY=1.354214
PRIMER_LEFT_42_SEQUENCE=AAAGATTGTTCCGAGACGTTG
PRIMER_RIGHT_42_SEQUENCE=TCTGCTTACAAGCTTCGTTCC
PRIMER_LEFT_42=416,21
PRIMER_RIGHT_42=1233,21
PRIMER_LEFT_42_TM=59.228
PRIMER_RIGHT_42_TM=59.646
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=4.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=8.7000
PRIMER_RIGHT_42_END_STABILITY=7.9000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=818
PRIMER_PAIR_43_PENALTY=3.164942
PRIMER_LEFT_43_PENALTY=2.039879
PRIMER_RIGHT_43_PENALTY=1.125062
PRIMER_LEFT_43_SEQUENCE=AAAGATTGTTCCGAGACGTTGT
PRIMER_RIGHT_43_SEQUENCE=CATTCTGCATTGACGTTCCTT
PRIMER_LEFT_43=416,22
PRIMER_RIGHT_43=1277,21
PRIMER_LEFT_43_TM=60.040
PRIMER_RIGHT_43_TM=60.125
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=42.857
PRIMER_LEFT_43_SELF_ANY=4.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=862
PRIMER_PAIR_44_PENALTY=3.173823
PRIMER_LEFT_44_PENALTY=2.048761
PRIMER_RIGHT_44_PENALTY=1.125062
PRIMER_LEFT_44_SEQUENCE=CTGCGATTCCAAAACACTCT
PRIMER_RIGHT_44_SEQUENCE=CATTCTGCATTGACGTTCCTT
PRIMER_LEFT_44=380,20
PRIMER_RIGHT_44=1277,21
PRIMER_LEFT_44_TM=57.951
PRIMER_RIGHT_44_TM=60.125
PRIMER_LEFT_44_GC_PERCENT=45.000
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=6.1000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=898
PRIMER_PAIR_45_PENALTY=3.189779
PRIMER_LEFT_45_PENALTY=2.039879
PRIMER_RIGHT_45_PENALTY=1.149900
PRIMER_LEFT_45_SEQUENCE=AAAGATTGTTCCGAGACGTTGT
PRIMER_RIGHT_45_SEQUENCE=TTCTGCATTGACGTTCCTTC
PRIMER_LEFT_45=416,22
PRIMER_RIGHT_45=1275,20
PRIMER_LEFT_45_TM=60.040
PRIMER_RIGHT_45_TM=58.850
PRIMER_LEFT_45_GC_PERCENT=40.909
PRIMER_RIGHT_45_GC_PERCENT=45.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=860
PRIMER_PAIR_46_PENALTY=3.198660
PRIMER_LEFT_46_PENALTY=2.048761
PRIMER_RIGHT_46_PENALTY=1.149900
PRIMER_LEFT_46_SEQUENCE=CTGCGATTCCAAAACACTCT
PRIMER_RIGHT_46_SEQUENCE=TTCTGCATTGACGTTCCTTC
PRIMER_LEFT_46=380,20
PRIMER_RIGHT_46=1275,20
PRIMER_LEFT_46_TM=57.951
PRIMER_RIGHT_46_TM=58.850
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.1000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=896
PRIMER_PAIR_47_PENALTY=3.234572
PRIMER_LEFT_47_PENALTY=2.109510
PRIMER_RIGHT_47_PENALTY=1.125062
PRIMER_LEFT_47_SEQUENCE=GGACAGAAAAGATTGTTCCGAG
PRIMER_RIGHT_47_SEQUENCE=CATTCTGCATTGACGTTCCTT
PRIMER_LEFT_47=409,22
PRIMER_RIGHT_47=1277,21
PRIMER_LEFT_47_TM=60.110
PRIMER_RIGHT_47_TM=60.125
PRIMER_LEFT_47_GC_PERCENT=45.455
PRIMER_RIGHT_47_GC_PERCENT=42.857
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=9.9000
PRIMER_RIGHT_47_END_STABILITY=8.2000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=869
PRIMER_PAIR_48_PENALTY=3.259410
PRIMER_LEFT_48_PENALTY=2.109510
PRIMER_RIGHT_48_PENALTY=1.149900
PRIMER_LEFT_48_SEQUENCE=GGACAGAAAAGATTGTTCCGAG
PRIMER_RIGHT_48_SEQUENCE=TTCTGCATTGACGTTCCTTC
PRIMER_LEFT_48=409,22
PRIMER_RIGHT_48=1275,20
PRIMER_LEFT_48_TM=60.110
PRIMER_RIGHT_48_TM=58.850
PRIMER_LEFT_48_GC_PERCENT=45.455
PRIMER_RIGHT_48_GC_PERCENT=45.000
PRIMER_LEFT_48_SELF_ANY=4.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=9.9000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=867
PRIMER_PAIR_49_PENALTY=3.271587
PRIMER_LEFT_49_PENALTY=2.146525
PRIMER_RIGHT_49_PENALTY=1.125062
PRIMER_LEFT_49_SEQUENCE=TCACGTTGGACAGAAAAGATTG
PRIMER_RIGHT_49_SEQUENCE=CATTCTGCATTGACGTTCCTT
PRIMER_LEFT_49=402,22
PRIMER_RIGHT_49=1277,21
PRIMER_LEFT_49_TM=60.147
PRIMER_RIGHT_49_TM=60.125
PRIMER_LEFT_49_GC_PERCENT=40.909
PRIMER_RIGHT_49_GC_PERCENT=42.857
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=6.9000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=2.00
PRIMER_PAIR_49_PRODUCT_SIZE=876
=
SEQUENCE_ID=MAGIC.338_2_5052617_5052973_wu-0_col-0_328964_489332_TRUE
SEQUENCE_TARGET=950,456
SEQUENCE_TEMPLATE=CTGTGAGTTTGGAGGACATTTGTATATATATGTGTGTTTGCGCAATTTATGGGTTATCTTTTGGACTAAATGAATTNANGGGTGCATACATATACATACATNAACATTTTTCTAACNTTTAAATAACACTTAAATTGATAANATTTTAGATNTGTTTTGGTCCCCTCTAAGNNANNAAANANATTACAAATCGTCCAAAAGTNTCTGTAGTTTTGATTAGTTTCTGNAAAAGAAGAATNATGAGGNNATTTTTNTTNTGTNTATGAGGGACACACATAAGGGACAAATAAANGAGNAACGAAAAATACGGCTTTAGGCTTTCTATATTTCTAAAGCAATAATNTAGTGGTTGTGACTTAGAGAGATTTCTTTTTCAACCACTCCATTTTCTTAAGTTCTCGGAAAACGAACATGTAATATGGNATGAATTNACATCACANTNNNNNNNNNTAGTAGTAATTTCTTCCTTTANACAGAGTGCNAAAAAANGGACNTTNCACCANAANAATAATGNAANANNNTANNAAGCCTAATNNCATANAACATTGTATTNGTTTTCTNTTGGTNTTGAGCATGGTGCCNAATTTAGTTCATANNNNNNNNNNNNTCNATGTTGTTATTNAATAANTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGCTTCCGTCATCAAAATTANGATTCNATATATTTNTTTNTCTTCCAAAAANTTCTCTAAGATCANCNGNTTAAACNACTTCTTTNGTAAGGTACAAGCGAGAANTCATANTATAGTAAAANAANNAAAAAAAAANTCATGTTATTTGGTACTTTTCGCAATNAACGGACCGCAAGTTTTTATATTGGTTCCATTTAAANNNAAATCCNNNNAGACTTTCTATCCAGCTCTTAANAAACTAACTATAACCCATAAAACNTGGACCACNATGTATAACTAATCAACAAATTAATTTGGTAGAGCAGATGCACAGTAGTCAATGCTCCACTCCCCAATTTNTTGGCCGAAAGTGATCCATTNAAGTGGTAGCCCCAAGNTGTGGTACCTGTCTTTAACCCAACCTCTACCTATATGCCTCTCTCNNCNCTTTCTCNTTGTCTTCNGCNTCACTTGATTTGGTGAATATGNTTNGNTTNAGTNTCTGNNTTTCACATGNTTATCTNGTTATGGGTTCTCTGTATGGGTGGGCATAATANCNTTTNCNGGAANNAAAAANNAAANNAACTGAANTGTAACTAGGTGATTTGGTTCNGTTTTGGTTNTCTTNAAAATNNATTTGGTTATCTTTACTAAAAATAATTCAGTTTCGATTGACATGGTTTAGAGCANTTNNTTTTTTGAGATTAGTGGGCTTTCAGGCCCAATGTTAGTGAGATNAGAGATTTTAGTACGTCTCACATTTGGTATTCGCTTCTGTTNNTTTTTTGNGTTTTAGATTTTAANAACTATATAACTACCAAAATATTTTTGTTAAGATCGGGTTCTTTCAGTTTTGGAGTTTATGATTCATTNGGATATTATCGGATATGTTTGGATATTTTAGGTATTTTTGGNTTNTTTTTCTNNGTATTNTTTTGATACTCNGGNNATTTTTGGTTATTCGGTTTNAAAANCTAATATTATTGTTTANAGTAAGTTGNAAATATANTCACTTTTCAAAANATAATTNTGNANNTAGTAACTTCACTATTCAANATTAGANAANGAGGTNANTTTNCNTTTTGTNTAATAGGCGGATATCTAATANNAGTTCNNNTNGNGCANNNAATAATTTTTGNAAATTACCNGAGAGGNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_3_10480482_10480536_oy-0_zu-0_7937_889_TRUE
SEQUENCE_TARGET=950,154
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_4_4695032_4695206_po-0_rsch-4_10486_4779_TRUE
SEQUENCE_TARGET=950,274
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_9444382_9444458_hi-0_bur-0_122694_1163_TRUE
SEQUENCE_TARGET=950,176
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNGATGGAATCTTTAGANCTANNATCCACCACCNGCAAATGGCAAGTGGTCAAATTAGCCTCAAATCNCAACCNGATGNAACCTACCGTCAATGTGCNACAAGGTCATTATGGGTTCCAACATATATTTTGTCAACANGANAGGNNTGNGNTGCANNGNGTTNGACTTGNANANAANAACACTTAAAATGGAGAGAAATNATGATGCANCTATGANAAACANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATNTATATNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNNNNNAAATGTGTTATTTTNNCAAATCACCNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAACACAAACANTTGGAANAAATCCAAAGGATGTCATCAATAAGCCATATCCACCATGNAAAATGTATANAATGGAATAACTAAAACACAAATCCAAAANATTNAACTTTAAATNNAAAAAAACTGATTAGAATCTAGGTTAAAATTTAGTTTTTNATCAANGTATNAAGAATGTATCTCTTCTTCACAACTTTANCATCTTCGTATANATCACATNTAAACTTTNAAAGTAAAAACATAAAGAAATTTACAAGATTTGGATANCTTCGAAGCCAACTTTTAATGAGTTNAGAACCTAAAAATTGTGGGAAGGACAACAACCAAATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGAGCATTCTGACACAAGACAGGACTATAGCCTAGTTCTGNTTTTTCTNNNNNNNNNNNNNNNNNNNNNNACATTAAAATCTGTGACATCTCACGTNCTCTCTCTCTCTCTTCTATCTCTAATTGTAGTTTGTATCCAANTCCACGCGCCTTTTTCCACTCTCTGTCNCCACTTTNCTT
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_4_8800802_8801291_can-0_oy-0_64502_7830_TRUE
SEQUENCE_TARGET=950,589
SEQUENCE_TEMPLATE=ACAACCGAGGAGTTGACTTAGTGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAAAGATAAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTCTTCGTTTGGTGTTTTGTAGTTTGATAGGCCTCTCTCTCTTGAGATTTCAGAACATCGATATTGACAAAATATACTAAACTAAGGCTCGATTGAGTAAAAACAACAAAATCAGTAATCAGATCTTGACTTCATCTGGTTTGTAATTTAANGACTTGNCAATTCACCTATTGCCCATGATCTAATTNTTTAAACCACCAGAAGTTGTATTGAACTGATTGATCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAACCNTTNNTNAATTGCTTAGAATNTTTNNNAAATTTTGTAAACTCTTTTCTNAATTTCAGNTTTTTTNGCAGGAAAATTTGAGANTAGTTTTCTACGNAAANNTTTTGGTGTTACCNGAAATNNTTTTGNGNAAAACTTTTAANGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTGAACCNAAAANAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_5409709_5409872_oy-0_ler-0_24528_39204_TRUE
SEQUENCE_TARGET=950,263
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_1_15728787_15728918_no-0_tsu-0_25789_86002_TRUE
SEQUENCE_TARGET=950,231
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCNATCGCGTTCACATGTGTAATTGACAATTTACAAATGACTNGATTAAGATACTATTTNATCAATTTATCTAGATAAGTTTCATGTCATTTNCTTNGACCACCCTTAANGGTAGTCTATTAACAGAAACTCTAAAAAANANATATTANAGAATAATCAAAAGCTGTNAAAAAAAAGGGAGAGAGAGTGAACAAAAATCCTTATCTAAGANTATTTATCACCANAAAAAAACANGTGTAANGTTANTAGTAATTTGTGTGTATTAACAAAATAAAATATAATCATAACATTAATATATTATTACGTTTTGGTTTTTAAAAAACTCTTACATTTTCATCNGTTGAAGATGCTCTTAAATATCTTTGTTATTTTTACTTGGGTTGTGTNNGNTTGAGACGCGAGAGTATTAATNTCTCTTTTTNTTTTTTNNNNNNNNNNNNNNANCTAGANAGACTANAGCCNNCATNATAGGAATAGGANAGATCCCANAANATACNGATTTNTATTACATNNAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_4_11038774_11039272_can-0_ler-0_34155_341658_TRUE
SEQUENCE_TARGET=950,598
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_10296751_10297370_wil-2_ct-1_684170_178992_TRUE
SEQUENCE_TARGET=950,719
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_17574697_17575013_ler-0_po-0_13076_45652_TRUE
SEQUENCE_TARGET=950,416
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGTAACATTTGTACNNACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTTTAGGACATACGTGCCAATGTTCTTCTCAAGCTGAATATTGAACANGTAAAGNAACCTTGCAAGACCCCATCTGATATCTTGAAAAGAAGGAAATGAGACATCCAACTTGAGAGGAGTAGGTAGAGAGTTNGATGCACAAGCAAAAGGCAAGCCAGCCCCCACTGAGTTCTGAACGAGGCNAGGAAGCTCTTGACATATCTTTTNCGCTATCCTGAACACCGCATACAGTGGGATGCATCCTNTCNCCACTTTATACNGAAGACTCTGCAGAATAAGAAGATTATATCATAAGCATACGGAATATAACATCTNCATAACTAAACAAGAAGCAACCACAATGAAAGACATCAAATNCACCAATAGANACAATGAGCTAAGACTCATCCTTCAAAGNCAGAAANATAAAGACATGACACTGGANATGGCTTAGCTTAACCTTATGNAATATGGAACTGAAATCATTCAGGTTGACACTAGAAGACGGNTCNGTGTCNCCTGTCCTCTGAGATTTGACTTTGAAGGTCCCTGTAGTANAGGAANAANATTAACAATNNGACCCTAAAGATTGCATCTNTACNNTAGCAAACCTCACCTGGGCATCTAAAAGACNCAATCCCACCTAAAGGCNNAGACTTTAAACTNAAGCNCCTGCAAAAATNAAACTTTCAGAGATNCACAACTTGTAAGCATTANATACCCAATCCGACTAAATGAGCNTTGATTGNATCATTTTAGCTTATTGTAGTCCCAAATTCANTTCGAACANAAACCCAAAACACAGATTCAGAGCTTCCATNAGAACCATTGTNATTTCNAATCAAAACCCATTAANNACTCTGCTNCATTNTATACANTAANNCAAACATGAATCAGCTTCATCGGAANACAGNAACCAAAANCCAAAATCTTCGACANTCACAAANNNNNNNNTCATCANNTGNTGGATAAGTNACACCCACCATGAAAGCAAGCAACTTTTNTCAGAAANTNNNAATTATAAGAAAAACCTGTTAAAAGACGCGAATCGATCTAGTCTTGAAGATTGTGAAGGTAGTAGAACCAATGGCTTCCAAGTAAACAACTGAACAGCNNCCATCATTTATCTCTTCTCNTCGNNCTCTCTNTNTTTCTCCGCTTANATATATATATGAGGCTGANCNTTACNTTTTGCACCTTTTTCCTTTNCGAGGGAAAGAAATGTGTATTNTAATTAGAGTCAATTATCAANNNTTGTTGTTAATGACTTNAATGTTTTGGACCATNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANANTCCTAATTTGNTCACACNAAANCNGTACTAANGCAANCAANCCTTTATATGTNANTCTGGATCATTANGTTGNTCACTTAGTTAGACAGCACAAANATCCAATTCACNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATATATACAAGAAACTANACNNNAANTATAGAGATTTTACTTGTTNATGACTTTATNTTTNTGATCATCNNAAAATTGCGTCTAANTTTTTTTCTTNTGATTAGAAGATTTTGGNCACAGNCCTAGANCATNTAAACATGTCNTTTTTACGAGCAAATGCAAAATCCTANAANNNTTTGGNTTNTNGTATACATGGNTGAGNNNNNNNNNNNNNNNNNNNNNNNNNTAAGTCAAGTCATTGTTTTTNAATTGTTACTTTTGGTCACCACTTTAATTNTNAAAGATGGACTGCGAGNAANCCTTTAACNTTNACTCAANNACACTCNTATNATTNAATTTATTTNTGGAAAACCACACAAAGTATNTTATTCACAACTCGAATAANANCAATCCTNAGAGAACGTGCTGCATAAANTTGAAACCAACATAGTAACTAACTAACAAAANNTTANTATATNCTATTCGACTTCAATATTATTTTNTTGNTNTTTAAATTAGGTATGACTTATGAGTTATGAGATGAGATCGAANNNGANGAAGAAGTGGAACACAAAGCACGTGGGTACANCACAATGTGAATC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_15847761_15847769_bur-0_can-0_58848_24901_TRUE
SEQUENCE_TARGET=950,108
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_2_3648706_3649097_hi-0_oy-0_1018492_101152_TRUE
SEQUENCE_TARGET=950,491
SEQUENCE_TEMPLATE=GATTTTTNCAAGTTCCACCTCTTTGAAAAATATAATCATTTNNGTGAAATCCAANTGTTGAGTTCGCTCTTCACAAGATTAGATTTCATTCCTCAAGGATTTTTCCAAGTTCCACCTNTTGGAAAAATGTATTCATTTCAGTGAAATCTAAGTGTTGAGTTCNNACTTCGCAAGACTAGATTATATCCCTCAAGGATATTTCCAAGTTTCACNTCTTGGAAANATATATTCANTTAGNTCGCTNTTNACAAGNCTAGATTTCATCCTTCAAGGATTTTTTCAAATTCCACCTNTTGGAAAAATATATTCATTTTGGTGAAATCTAAGTGTTGAGTTTGCTATTCAAAAGACAAGATTTCATCCCTNAAAAAGTTTTCCAAGTTCCACNGCTCGGAAAAATATATTCATTTTGGTGAAATCTAAGTGTTGAGTTCGCTCTTCACAAGACTAGATTTCATCCCTCAAGGATTTTTCCAAGTTTCACCTCTTGAAAAAATGTATTCNTTTNAGCGAAATNTAANTGTTGANTTCGCTCTTCACAAGACTATATTTCATCCCTCAAAGATTTTTCCAAGTTCCACCTCTTGGAAAAATATATTCATTTCGGTGAAATCTAAGTGTTGAGTTCGCGCTTCACAAGACTAGATTTCATCCGTCAAGGATTTTTCCAAGTTCCACNTCTTGAAAATTATATTCANTTTGGTGAAATCTAAGTNTTGAGGTTGCTCTTCACATNGCTNNANTTTNATCCCTCAAANATGNTTTTGATGTCCGACCANCGNAAGAATGTGCNCNTTTCNATGGAATTTATGTATCGATTTAACTCGTCATGGACTCAAATTCCAGTTAAAGCGGTGGCGATTCACTTTTTCTCNGANGCAAGAAACTCGTAAAAGNAANNNAAAAAANAAAGAGGCCATCAACGTCTCATGATNTTCCGAGATTTCCCATTAGNTACACAAACTTCACCAAAGAACTCCTNGACCTCAACAAGACTACANCTCGTGGTGGCGAAACAACAACNTTGTCACCTCCAAAGTAGGANCACAAGTCTAGGTAGAAGCAAGGNTGAGTCTACTAAGATCAACTCAATCGATCAAGCCATGACACAATGATACAGAGAAGTTTTCATGGCGTTTCTTCGGAAATCNTTAGCGNATGACTTCAACAATTACAGCCATGANAGGACANGATCATATTGGCTCAAAACATTTGGATCAGATCATGTATGAAGCATCAAAGATAAGTTCAACTATGNTGTANACGTTTACTCCTTAACNGGAATGTCTCCATCTTAAGNTACACAAAATGTTATGACGAAAAGCATAGATGTCTAAGACCNAACAGGTGGGGGCNTCTTCCAAATAATATAAAGCAAAGTCAAGCCTCTTNNGAGGAGAATTATACAGCATTATCACTTAACGTGATTGACAAATCATTNCTACAAGTTTGGAGTCCATATTTATATCATCACTCAAAATTCAAGGAGTCAAAATGCGANATTTGTCAACNNNAAGAGATTCAAGTTTGAAAAAGACAACTTCGATGCTATACCACAACCTTCNAATGTTATTCACCTTCATGGAGATGTGAANTNCAATNGGTGGAATCTAAGTGTTGNGTTTGCTNNNNNNGAAATTAGATTCCATCGAGAAGAGATTTCTCNAANTTCTANCTCTTGGAAAAATGTGTTCATTNGATGGAATTTAAGAGNTTTTTCNATAGTTTACCTNCTTTATTNNGAGNAATCAATCGGATANAAGTGGAAGNCGAGAAGATTAGCTTCACTTCGCAAGAAAANGAAAGCCGANTATGCCAATTGTTACTGTCCCTTNCAGGAAACCAANGTTAAAGNGCAACAAAGAGATCAAACCACTTGGATTAAACCCATTTAACAATAATGTGGAGACANCATCTGTAANACCCCTATACCGCTATANAAATATAAACGAAATAGGANNNTNACGTTAGACGCCCGNAGTCGGCCAATCNAGGTTTNCAAAATGAATTNGTTCGCCTNAGTCGTCNAGCCGCNAGAGTCTAGCCTCCCGCCCNTGACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=4.036417
PRIMER_LEFT_0_PENALTY=0.825798
PRIMER_RIGHT_0_PENALTY=3.210619
PRIMER_LEFT_0_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_0_SEQUENCE=TTCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_0=853,20
PRIMER_RIGHT_0=1797,22
PRIMER_LEFT_0_TM=59.174
PRIMER_RIGHT_0_TM=58.789
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=40.909
PRIMER_LEFT_0_SELF_ANY=3.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=7.0000
PRIMER_RIGHT_0_END_STABILITY=5.9000
PRIMER_PAIR_0_COMPL_ANY=7.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=945
PRIMER_PAIR_1_PENALTY=4.123185
PRIMER_LEFT_1_PENALTY=0.912566
PRIMER_RIGHT_1_PENALTY=3.210619
PRIMER_LEFT_1_SEQUENCE=AATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_1_SEQUENCE=TTCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_1=838,20
PRIMER_RIGHT_1=1797,22
PRIMER_LEFT_1_TM=59.087
PRIMER_RIGHT_1_TM=58.789
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=960
PRIMER_PAIR_2_PENALTY=4.300436
PRIMER_LEFT_2_PENALTY=0.825798
PRIMER_RIGHT_2_PENALTY=3.474638
PRIMER_LEFT_2_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_2_SEQUENCE=TTCTTGCGAAGTGAAGCTAA
PRIMER_LEFT_2=853,20
PRIMER_RIGHT_2=1797,20
PRIMER_LEFT_2_TM=59.174
PRIMER_RIGHT_2_TM=56.525
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=945
PRIMER_PAIR_3_PENALTY=4.387204
PRIMER_LEFT_3_PENALTY=0.912566
PRIMER_RIGHT_3_PENALTY=3.474638
PRIMER_LEFT_3_SEQUENCE=AATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_3_SEQUENCE=TTCTTGCGAAGTGAAGCTAA
PRIMER_LEFT_3=838,20
PRIMER_RIGHT_3=1797,20
PRIMER_LEFT_3_TM=59.087
PRIMER_RIGHT_3_TM=56.525
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=960
PRIMER_PAIR_4_PENALTY=4.417917
PRIMER_LEFT_4_PENALTY=0.825798
PRIMER_RIGHT_4_PENALTY=3.592119
PRIMER_LEFT_4_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_4_SEQUENCE=TCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_4=853,20
PRIMER_RIGHT_4=1796,21
PRIMER_LEFT_4_TM=59.174
PRIMER_RIGHT_4_TM=57.408
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=944
PRIMER_PAIR_5_PENALTY=4.504685
PRIMER_LEFT_5_PENALTY=0.912566
PRIMER_RIGHT_5_PENALTY=3.592119
PRIMER_LEFT_5_SEQUENCE=AATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_5_SEQUENCE=TCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_5=838,20
PRIMER_RIGHT_5=1796,21
PRIMER_LEFT_5_TM=59.087
PRIMER_RIGHT_5_TM=57.408
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=5.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=959
PRIMER_PAIR_6_PENALTY=4.541171
PRIMER_LEFT_6_PENALTY=0.459180
PRIMER_RIGHT_6_PENALTY=4.081990
PRIMER_LEFT_6_SEQUENCE=CCGTCAAGGATTTTTCCAAG
PRIMER_RIGHT_6_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_6=652,20
PRIMER_RIGHT_6=1557,24
PRIMER_LEFT_6_TM=59.541
PRIMER_RIGHT_6_TM=60.082
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=41.667
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=906
PRIMER_PAIR_7_PENALTY=4.560561
PRIMER_LEFT_7_PENALTY=1.349942
PRIMER_RIGHT_7_PENALTY=3.210619
PRIMER_LEFT_7_SEQUENCE=AAATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_7_SEQUENCE=TTCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_7=837,21
PRIMER_RIGHT_7=1797,22
PRIMER_LEFT_7_TM=60.350
PRIMER_RIGHT_7_TM=58.789
PRIMER_LEFT_7_GC_PERCENT=42.857
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=5.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PAIR_7_PRODUCT_SIZE=961
PRIMER_PAIR_8_PENALTY=4.577070
PRIMER_LEFT_8_PENALTY=1.366451
PRIMER_RIGHT_8_PENALTY=3.210619
PRIMER_LEFT_8_SEQUENCE=CAAATTCCAGTTAAAGCGGTG
PRIMER_RIGHT_8_SEQUENCE=TTCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_8=836,21
PRIMER_RIGHT_8=1797,22
PRIMER_LEFT_8_TM=59.634
PRIMER_RIGHT_8_TM=58.789
PRIMER_LEFT_8_GC_PERCENT=42.857
PRIMER_RIGHT_8_GC_PERCENT=40.909
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=9.9000
PRIMER_RIGHT_8_END_STABILITY=5.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=962
PRIMER_PAIR_9_PENALTY=4.662548
PRIMER_LEFT_9_PENALTY=0.580558
PRIMER_RIGHT_9_PENALTY=4.081990
PRIMER_LEFT_9_SEQUENCE=AAGTGTTGAGTTCGCGCTTC
PRIMER_RIGHT_9_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_9=615,20
PRIMER_RIGHT_9=1557,24
PRIMER_LEFT_9_TM=60.581
PRIMER_RIGHT_9_TM=60.082
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=41.667
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=943
PRIMER_PAIR_10_PENALTY=4.824579
PRIMER_LEFT_10_PENALTY=1.349942
PRIMER_RIGHT_10_PENALTY=3.474638
PRIMER_LEFT_10_SEQUENCE=AAATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_10_SEQUENCE=TTCTTGCGAAGTGAAGCTAA
PRIMER_LEFT_10=837,21
PRIMER_RIGHT_10=1797,20
PRIMER_LEFT_10_TM=60.350
PRIMER_RIGHT_10_TM=56.525
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=40.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=7.5000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=961
PRIMER_PAIR_11_PENALTY=4.841089
PRIMER_LEFT_11_PENALTY=1.366451
PRIMER_RIGHT_11_PENALTY=3.474638
PRIMER_LEFT_11_SEQUENCE=CAAATTCCAGTTAAAGCGGTG
PRIMER_RIGHT_11_SEQUENCE=TTCTTGCGAAGTGAAGCTAA
PRIMER_LEFT_11=836,21
PRIMER_RIGHT_11=1797,20
PRIMER_LEFT_11_TM=59.634
PRIMER_RIGHT_11_TM=56.525
PRIMER_LEFT_11_GC_PERCENT=42.857
PRIMER_RIGHT_11_GC_PERCENT=40.000
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.9000
PRIMER_RIGHT_11_END_STABILITY=7.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=962
PRIMER_PAIR_12_PENALTY=4.907788
PRIMER_LEFT_12_PENALTY=0.825798
PRIMER_RIGHT_12_PENALTY=4.081990
PRIMER_LEFT_12_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_12_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_12=853,20
PRIMER_RIGHT_12=1557,24
PRIMER_LEFT_12_TM=59.174
PRIMER_RIGHT_12_TM=60.082
PRIMER_LEFT_12_GC_PERCENT=45.000
PRIMER_RIGHT_12_GC_PERCENT=41.667
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=705
PRIMER_PAIR_13_PENALTY=4.942060
PRIMER_LEFT_13_PENALTY=1.349942
PRIMER_RIGHT_13_PENALTY=3.592119
PRIMER_LEFT_13_SEQUENCE=AAATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_13_SEQUENCE=TCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_13=837,21
PRIMER_RIGHT_13=1796,21
PRIMER_LEFT_13_TM=60.350
PRIMER_RIGHT_13_TM=57.408
PRIMER_LEFT_13_GC_PERCENT=42.857
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=5.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=960
PRIMER_PAIR_14_PENALTY=4.958570
PRIMER_LEFT_14_PENALTY=1.366451
PRIMER_RIGHT_14_PENALTY=3.592119
PRIMER_LEFT_14_SEQUENCE=CAAATTCCAGTTAAAGCGGTG
PRIMER_RIGHT_14_SEQUENCE=TCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_14=836,21
PRIMER_RIGHT_14=1796,21
PRIMER_LEFT_14_TM=59.634
PRIMER_RIGHT_14_TM=57.408
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=42.857
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.9000
PRIMER_RIGHT_14_END_STABILITY=5.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=961
PRIMER_PAIR_15_PENALTY=4.994556
PRIMER_LEFT_15_PENALTY=0.912566
PRIMER_RIGHT_15_PENALTY=4.081990
PRIMER_LEFT_15_SEQUENCE=AATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_15_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_15=838,20
PRIMER_RIGHT_15=1557,24
PRIMER_LEFT_15_TM=59.087
PRIMER_RIGHT_15_TM=60.082
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=41.667
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=720
PRIMER_PAIR_16_PENALTY=5.193307
PRIMER_LEFT_16_PENALTY=1.982688
PRIMER_RIGHT_16_PENALTY=3.210619
PRIMER_LEFT_16_SEQUENCE=GGTGGCGATTCACTTTTTCTC
PRIMER_RIGHT_16_SEQUENCE=TTCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_16=853,21
PRIMER_RIGHT_16=1797,22
PRIMER_LEFT_16_TM=60.983
PRIMER_RIGHT_16_TM=58.789
PRIMER_LEFT_16_GC_PERCENT=47.619
PRIMER_RIGHT_16_GC_PERCENT=40.909
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=5.9000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=945
PRIMER_PAIR_17_PENALTY=5.377132
PRIMER_LEFT_17_PENALTY=0.825798
PRIMER_RIGHT_17_PENALTY=4.551334
PRIMER_LEFT_17_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_17_SEQUENCE=CTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_17=853,20
PRIMER_RIGHT_17=1795,20
PRIMER_LEFT_17_TM=59.174
PRIMER_RIGHT_17_TM=55.449
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=5.9000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PAIR_17_PRODUCT_SIZE=943
PRIMER_PAIR_18_PENALTY=5.383722
PRIMER_LEFT_18_PENALTY=0.459180
PRIMER_RIGHT_18_PENALTY=4.924542
PRIMER_LEFT_18_SEQUENCE=CCGTCAAGGATTTTTCCAAG
PRIMER_RIGHT_18_SEQUENCE=GCATCGAAGTTGTCTTTTTC
PRIMER_LEFT_18=652,20
PRIMER_RIGHT_18=1548,20
PRIMER_LEFT_18_TM=59.541
PRIMER_RIGHT_18_TM=55.075
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=40.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=897
PRIMER_PAIR_19_PENALTY=5.420341
PRIMER_LEFT_19_PENALTY=1.338351
PRIMER_RIGHT_19_PENALTY=4.081990
PRIMER_LEFT_19_SEQUENCE=CCGTCAAGGATTTTTCCAAGT
PRIMER_RIGHT_19_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_19=652,21
PRIMER_RIGHT_19=1557,24
PRIMER_LEFT_19_TM=60.338
PRIMER_RIGHT_19_TM=60.082
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=41.667
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=906
PRIMER_PAIR_20_PENALTY=5.431932
PRIMER_LEFT_20_PENALTY=1.349942
PRIMER_RIGHT_20_PENALTY=4.081990
PRIMER_LEFT_20_SEQUENCE=AAATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_20_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_20=837,21
PRIMER_RIGHT_20=1557,24
PRIMER_LEFT_20_TM=60.350
PRIMER_RIGHT_20_TM=60.082
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=41.667
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=721
PRIMER_PAIR_21_PENALTY=5.448442
PRIMER_LEFT_21_PENALTY=1.366451
PRIMER_RIGHT_21_PENALTY=4.081990
PRIMER_LEFT_21_SEQUENCE=CAAATTCCAGTTAAAGCGGTG
PRIMER_RIGHT_21_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_21=836,21
PRIMER_RIGHT_21=1557,24
PRIMER_LEFT_21_TM=59.634
PRIMER_RIGHT_21_TM=60.082
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=41.667
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=9.9000
PRIMER_RIGHT_21_END_STABILITY=6.4000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=722
PRIMER_PAIR_22_PENALTY=5.457325
PRIMER_LEFT_22_PENALTY=1.982688
PRIMER_RIGHT_22_PENALTY=3.474638
PRIMER_LEFT_22_SEQUENCE=GGTGGCGATTCACTTTTTCTC
PRIMER_RIGHT_22_SEQUENCE=TTCTTGCGAAGTGAAGCTAA
PRIMER_LEFT_22=853,21
PRIMER_RIGHT_22=1797,20
PRIMER_LEFT_22_TM=60.983
PRIMER_RIGHT_22_TM=56.525
PRIMER_LEFT_22_GC_PERCENT=47.619
PRIMER_RIGHT_22_GC_PERCENT=40.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=7.5000
PRIMER_PAIR_22_COMPL_ANY=7.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=945
PRIMER_PAIR_23_PENALTY=5.463900
PRIMER_LEFT_23_PENALTY=0.912566
PRIMER_RIGHT_23_PENALTY=4.551334
PRIMER_LEFT_23_SEQUENCE=AATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_23_SEQUENCE=CTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_23=838,20
PRIMER_RIGHT_23=1795,20
PRIMER_LEFT_23_TM=59.087
PRIMER_RIGHT_23_TM=55.449
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=1.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=5.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=958
PRIMER_PAIR_24_PENALTY=5.482718
PRIMER_LEFT_24_PENALTY=0.459180
PRIMER_RIGHT_24_PENALTY=5.023538
PRIMER_LEFT_24_SEQUENCE=CCGTCAAGGATTTTTCCAAG
PRIMER_RIGHT_24_SEQUENCE=GTGGTATAGCATCGAAGTTGTCTTT
PRIMER_LEFT_24=652,20
PRIMER_RIGHT_24=1556,25
PRIMER_LEFT_24_TM=59.541
PRIMER_RIGHT_24_TM=59.976
PRIMER_LEFT_24_GC_PERCENT=45.000
PRIMER_RIGHT_24_GC_PERCENT=40.000
PRIMER_LEFT_24_SELF_ANY=3.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=8.5000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=905
PRIMER_PAIR_25_PENALTY=5.505099
PRIMER_LEFT_25_PENALTY=0.580558
PRIMER_RIGHT_25_PENALTY=4.924542
PRIMER_LEFT_25_SEQUENCE=AAGTGTTGAGTTCGCGCTTC
PRIMER_RIGHT_25_SEQUENCE=GCATCGAAGTTGTCTTTTTC
PRIMER_LEFT_25=615,20
PRIMER_RIGHT_25=1548,20
PRIMER_LEFT_25_TM=60.581
PRIMER_RIGHT_25_TM=55.075
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=40.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=1.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.3000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=934
PRIMER_PAIR_26_PENALTY=5.574806
PRIMER_LEFT_26_PENALTY=1.982688
PRIMER_RIGHT_26_PENALTY=3.592119
PRIMER_LEFT_26_SEQUENCE=GGTGGCGATTCACTTTTTCTC
PRIMER_RIGHT_26_SEQUENCE=TCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_26=853,21
PRIMER_RIGHT_26=1796,21
PRIMER_LEFT_26_TM=60.983
PRIMER_RIGHT_26_TM=57.408
PRIMER_LEFT_26_GC_PERCENT=47.619
PRIMER_RIGHT_26_GC_PERCENT=42.857
PRIMER_LEFT_26_SELF_ANY=3.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=1.00
PRIMER_LEFT_26_END_STABILITY=6.7000
PRIMER_RIGHT_26_END_STABILITY=5.9000
PRIMER_PAIR_26_COMPL_ANY=7.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PAIR_26_PRODUCT_SIZE=944
PRIMER_PAIR_27_PENALTY=5.604096
PRIMER_LEFT_27_PENALTY=0.580558
PRIMER_RIGHT_27_PENALTY=5.023538
PRIMER_LEFT_27_SEQUENCE=AAGTGTTGAGTTCGCGCTTC
PRIMER_RIGHT_27_SEQUENCE=GTGGTATAGCATCGAAGTTGTCTTT
PRIMER_LEFT_27=615,20
PRIMER_RIGHT_27=1556,25
PRIMER_LEFT_27_TM=60.581
PRIMER_RIGHT_27_TM=59.976
PRIMER_LEFT_27_GC_PERCENT=50.000
PRIMER_RIGHT_27_GC_PERCENT=40.000
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=1.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=7.0000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=942
PRIMER_PAIR_28_PENALTY=5.651340
PRIMER_LEFT_28_PENALTY=1.569350
PRIMER_RIGHT_28_PENALTY=4.081990
PRIMER_LEFT_28_SEQUENCE=TAAGTGTTGAGTTCGCGCTTC
PRIMER_RIGHT_28_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_28=614,21
PRIMER_RIGHT_28=1557,24
PRIMER_LEFT_28_TM=60.569
PRIMER_RIGHT_28_TM=60.082
PRIMER_LEFT_28_GC_PERCENT=47.619
PRIMER_RIGHT_28_GC_PERCENT=41.667
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=944
PRIMER_PAIR_29_PENALTY=5.750340
PRIMER_LEFT_29_PENALTY=0.825798
PRIMER_RIGHT_29_PENALTY=4.924542
PRIMER_LEFT_29_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_29_SEQUENCE=GCATCGAAGTTGTCTTTTTC
PRIMER_LEFT_29=853,20
PRIMER_RIGHT_29=1548,20
PRIMER_LEFT_29_TM=59.174
PRIMER_RIGHT_29_TM=55.075
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=40.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=7.0000
PRIMER_RIGHT_29_END_STABILITY=7.3000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=696
PRIMER_PAIR_30_PENALTY=5.820774
PRIMER_LEFT_30_PENALTY=2.610155
PRIMER_RIGHT_30_PENALTY=3.210619
PRIMER_LEFT_30_SEQUENCE=GTGGCGATTCACTTTTTCTC
PRIMER_RIGHT_30_SEQUENCE=TTCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_30=854,20
PRIMER_RIGHT_30=1797,22
PRIMER_LEFT_30_TM=57.390
PRIMER_RIGHT_30_TM=58.789
PRIMER_LEFT_30_GC_PERCENT=45.000
PRIMER_RIGHT_30_GC_PERCENT=40.909
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.7000
PRIMER_RIGHT_30_END_STABILITY=5.9000
PRIMER_PAIR_30_COMPL_ANY=7.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=944
PRIMER_PAIR_31_PENALTY=5.837108
PRIMER_LEFT_31_PENALTY=0.912566
PRIMER_RIGHT_31_PENALTY=4.924542
PRIMER_LEFT_31_SEQUENCE=AATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_31_SEQUENCE=GCATCGAAGTTGTCTTTTTC
PRIMER_LEFT_31=838,20
PRIMER_RIGHT_31=1548,20
PRIMER_LEFT_31_TM=59.087
PRIMER_RIGHT_31_TM=55.075
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=40.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=7.3000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=711
PRIMER_PAIR_32_PENALTY=5.849336
PRIMER_LEFT_32_PENALTY=0.825798
PRIMER_RIGHT_32_PENALTY=5.023538
PRIMER_LEFT_32_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_32_SEQUENCE=GTGGTATAGCATCGAAGTTGTCTTT
PRIMER_LEFT_32=853,20
PRIMER_RIGHT_32=1556,25
PRIMER_LEFT_32_TM=59.174
PRIMER_RIGHT_32_TM=59.976
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=40.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=7.0000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=704
PRIMER_PAIR_33_PENALTY=5.881244
PRIMER_LEFT_33_PENALTY=1.799253
PRIMER_RIGHT_33_PENALTY=4.081990
PRIMER_LEFT_33_SEQUENCE=TTTTCCAAGTTCCACCTCTTG
PRIMER_RIGHT_33_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_33=566,21
PRIMER_RIGHT_33=1557,24
PRIMER_LEFT_33_TM=59.201
PRIMER_RIGHT_33_TM=60.082
PRIMER_LEFT_33_GC_PERCENT=42.857
PRIMER_RIGHT_33_GC_PERCENT=41.667
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=1.00
PRIMER_LEFT_33_END_STABILITY=7.0000
PRIMER_RIGHT_33_END_STABILITY=6.4000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=992
PRIMER_PAIR_34_PENALTY=5.888509
PRIMER_LEFT_34_PENALTY=0.459180
PRIMER_RIGHT_34_PENALTY=5.429329
PRIMER_LEFT_34_SEQUENCE=CCGTCAAGGATTTTTCCAAG
PRIMER_RIGHT_34_SEQUENCE=TGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_34=652,20
PRIMER_RIGHT_34=1555,22
PRIMER_LEFT_34_TM=59.541
PRIMER_RIGHT_34_TM=56.571
PRIMER_LEFT_34_GC_PERCENT=45.000
PRIMER_RIGHT_34_GC_PERCENT=40.909
PRIMER_LEFT_34_SELF_ANY=3.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=6.4000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PAIR_34_PRODUCT_SIZE=904
PRIMER_PAIR_35_PENALTY=5.901276
PRIMER_LEFT_35_PENALTY=1.349942
PRIMER_RIGHT_35_PENALTY=4.551334
PRIMER_LEFT_35_SEQUENCE=AAATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_35_SEQUENCE=CTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_35=837,21
PRIMER_RIGHT_35=1795,20
PRIMER_LEFT_35_TM=60.350
PRIMER_RIGHT_35_TM=55.449
PRIMER_LEFT_35_GC_PERCENT=42.857
PRIMER_RIGHT_35_GC_PERCENT=45.000
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=9.4000
PRIMER_RIGHT_35_END_STABILITY=5.9000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=959
PRIMER_PAIR_36_PENALTY=5.917785
PRIMER_LEFT_36_PENALTY=1.366451
PRIMER_RIGHT_36_PENALTY=4.551334
PRIMER_LEFT_36_SEQUENCE=CAAATTCCAGTTAAAGCGGTG
PRIMER_RIGHT_36_SEQUENCE=CTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_36=836,21
PRIMER_RIGHT_36=1795,20
PRIMER_LEFT_36_TM=59.634
PRIMER_RIGHT_36_TM=55.449
PRIMER_LEFT_36_GC_PERCENT=42.857
PRIMER_RIGHT_36_GC_PERCENT=45.000
PRIMER_LEFT_36_SELF_ANY=4.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=1.00
PRIMER_LEFT_36_END_STABILITY=9.9000
PRIMER_RIGHT_36_END_STABILITY=5.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=960
PRIMER_PAIR_37_PENALTY=5.936104
PRIMER_LEFT_37_PENALTY=0.912566
PRIMER_RIGHT_37_PENALTY=5.023538
PRIMER_LEFT_37_SEQUENCE=AATTCCAGTTAAAGCGGTGG
PRIMER_RIGHT_37_SEQUENCE=GTGGTATAGCATCGAAGTTGTCTTT
PRIMER_LEFT_37=838,20
PRIMER_RIGHT_37=1556,25
PRIMER_LEFT_37_TM=59.087
PRIMER_RIGHT_37_TM=59.976
PRIMER_LEFT_37_GC_PERCENT=45.000
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=1.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=7.0000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=719
PRIMER_PAIR_38_PENALTY=5.987250
PRIMER_LEFT_38_PENALTY=0.459180
PRIMER_RIGHT_38_PENALTY=5.528069
PRIMER_LEFT_38_SEQUENCE=CCGTCAAGGATTTTTCCAAG
PRIMER_RIGHT_38_SEQUENCE=GTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_38=652,20
PRIMER_RIGHT_38=1556,23
PRIMER_LEFT_38_TM=59.541
PRIMER_RIGHT_38_TM=57.472
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=43.478
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=905
PRIMER_PAIR_39_PENALTY=6.006114
PRIMER_LEFT_39_PENALTY=2.795495
PRIMER_RIGHT_39_PENALTY=3.210619
PRIMER_LEFT_39_SEQUENCE=CGGTGGCGATTCACTTTTTC
PRIMER_RIGHT_39_SEQUENCE=TTCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_39=852,20
PRIMER_RIGHT_39=1797,22
PRIMER_LEFT_39_TM=62.795
PRIMER_RIGHT_39_TM=58.789
PRIMER_LEFT_39_GC_PERCENT=50.000
PRIMER_RIGHT_39_GC_PERCENT=40.909
PRIMER_LEFT_39_SELF_ANY=3.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=1.00
PRIMER_LEFT_39_END_STABILITY=7.3000
PRIMER_RIGHT_39_END_STABILITY=5.9000
PRIMER_PAIR_39_COMPL_ANY=7.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=946
PRIMER_PAIR_40_PENALTY=6.009886
PRIMER_LEFT_40_PENALTY=0.580558
PRIMER_RIGHT_40_PENALTY=5.429329
PRIMER_LEFT_40_SEQUENCE=AAGTGTTGAGTTCGCGCTTC
PRIMER_RIGHT_40_SEQUENCE=TGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_40=615,20
PRIMER_RIGHT_40=1555,22
PRIMER_LEFT_40_TM=60.581
PRIMER_RIGHT_40_TM=56.571
PRIMER_LEFT_40_GC_PERCENT=50.000
PRIMER_RIGHT_40_GC_PERCENT=40.909
PRIMER_LEFT_40_SELF_ANY=4.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=1.00
PRIMER_LEFT_40_END_STABILITY=8.2000
PRIMER_RIGHT_40_END_STABILITY=6.4000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=941
PRIMER_PAIR_41_PENALTY=6.052276
PRIMER_LEFT_41_PENALTY=1.127734
PRIMER_RIGHT_41_PENALTY=4.924542
PRIMER_LEFT_41_SEQUENCE=GAGTTCGCGCTTCACAAGAC
PRIMER_RIGHT_41_SEQUENCE=GCATCGAAGTTGTCTTTTTC
PRIMER_LEFT_41=622,20
PRIMER_RIGHT_41=1548,20
PRIMER_LEFT_41_TM=61.128
PRIMER_RIGHT_41_TM=55.075
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=40.000
PRIMER_LEFT_41_SELF_ANY=4.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.3000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=927
PRIMER_PAIR_42_PENALTY=6.064678
PRIMER_LEFT_42_PENALTY=1.982688
PRIMER_RIGHT_42_PENALTY=4.081990
PRIMER_LEFT_42_SEQUENCE=GGTGGCGATTCACTTTTTCTC
PRIMER_RIGHT_42_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_42=853,21
PRIMER_RIGHT_42=1557,24
PRIMER_LEFT_42_TM=60.983
PRIMER_RIGHT_42_TM=60.082
PRIMER_LEFT_42_GC_PERCENT=47.619
PRIMER_RIGHT_42_GC_PERCENT=41.667
PRIMER_LEFT_42_SELF_ANY=3.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=1.00
PRIMER_LEFT_42_END_STABILITY=6.7000
PRIMER_RIGHT_42_END_STABILITY=6.4000
PRIMER_PAIR_42_COMPL_ANY=4.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=705
PRIMER_PAIR_43_PENALTY=6.084793
PRIMER_LEFT_43_PENALTY=2.610155
PRIMER_RIGHT_43_PENALTY=3.474638
PRIMER_LEFT_43_SEQUENCE=GTGGCGATTCACTTTTTCTC
PRIMER_RIGHT_43_SEQUENCE=TTCTTGCGAAGTGAAGCTAA
PRIMER_LEFT_43=854,20
PRIMER_RIGHT_43=1797,20
PRIMER_LEFT_43_TM=57.390
PRIMER_RIGHT_43_TM=56.525
PRIMER_LEFT_43_GC_PERCENT=45.000
PRIMER_RIGHT_43_GC_PERCENT=40.000
PRIMER_LEFT_43_SELF_ANY=3.00
PRIMER_RIGHT_43_SELF_ANY=4.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=1.00
PRIMER_LEFT_43_END_STABILITY=6.7000
PRIMER_RIGHT_43_END_STABILITY=7.5000
PRIMER_PAIR_43_COMPL_ANY=7.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=944
PRIMER_PAIR_44_PENALTY=6.101795
PRIMER_LEFT_44_PENALTY=2.019805
PRIMER_RIGHT_44_PENALTY=4.081990
PRIMER_LEFT_44_SEQUENCE=CGTCAAGGATTTTTCCAAGTTC
PRIMER_RIGHT_44_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_44=653,22
PRIMER_RIGHT_44=1557,24
PRIMER_LEFT_44_TM=59.980
PRIMER_RIGHT_44_TM=60.082
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=41.667
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=6.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=905
PRIMER_PAIR_45_PENALTY=6.108627
PRIMER_LEFT_45_PENALTY=0.580558
PRIMER_RIGHT_45_PENALTY=5.528069
PRIMER_LEFT_45_SEQUENCE=AAGTGTTGAGTTCGCGCTTC
PRIMER_RIGHT_45_SEQUENCE=GTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_45=615,20
PRIMER_RIGHT_45=1556,23
PRIMER_LEFT_45_TM=60.581
PRIMER_RIGHT_45_TM=57.472
PRIMER_LEFT_45_GC_PERCENT=50.000
PRIMER_RIGHT_45_GC_PERCENT=43.478
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=8.2000
PRIMER_RIGHT_45_END_STABILITY=6.4000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=942
PRIMER_PAIR_46_PENALTY=6.202274
PRIMER_LEFT_46_PENALTY=2.610155
PRIMER_RIGHT_46_PENALTY=3.592119
PRIMER_LEFT_46_SEQUENCE=GTGGCGATTCACTTTTTCTC
PRIMER_RIGHT_46_SEQUENCE=TCTTGCGAAGTGAAGCTAATC
PRIMER_LEFT_46=854,20
PRIMER_RIGHT_46=1796,21
PRIMER_LEFT_46_TM=57.390
PRIMER_RIGHT_46_TM=57.408
PRIMER_LEFT_46_GC_PERCENT=45.000
PRIMER_RIGHT_46_GC_PERCENT=42.857
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.7000
PRIMER_RIGHT_46_END_STABILITY=5.9000
PRIMER_PAIR_46_COMPL_ANY=7.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=943
PRIMER_PAIR_47_PENALTY=6.231507
PRIMER_LEFT_47_PENALTY=2.149516
PRIMER_RIGHT_47_PENALTY=4.081990
PRIMER_LEFT_47_SEQUENCE=CAAGGATTTTTCCAAGTTCCAC
PRIMER_RIGHT_47_SEQUENCE=TGTGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_47=656,22
PRIMER_RIGHT_47=1557,24
PRIMER_LEFT_47_TM=59.850
PRIMER_RIGHT_47_TM=60.082
PRIMER_LEFT_47_GC_PERCENT=40.909
PRIMER_RIGHT_47_GC_PERCENT=41.667
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=7.9000
PRIMER_RIGHT_47_END_STABILITY=6.4000
PRIMER_PAIR_47_COMPL_ANY=5.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=902
PRIMER_PAIR_48_PENALTY=6.255127
PRIMER_LEFT_48_PENALTY=0.825798
PRIMER_RIGHT_48_PENALTY=5.429329
PRIMER_LEFT_48_SEQUENCE=GGTGGCGATTCACTTTTTCT
PRIMER_RIGHT_48_SEQUENCE=TGGTATAGCATCGAAGTTGTCT
PRIMER_LEFT_48=853,20
PRIMER_RIGHT_48=1555,22
PRIMER_LEFT_48_TM=59.174
PRIMER_RIGHT_48_TM=56.571
PRIMER_LEFT_48_GC_PERCENT=45.000
PRIMER_RIGHT_48_GC_PERCENT=40.909
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.0000
PRIMER_RIGHT_48_END_STABILITY=6.4000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=703
PRIMER_PAIR_49_PENALTY=6.262893
PRIMER_LEFT_49_PENALTY=1.338351
PRIMER_RIGHT_49_PENALTY=4.924542
PRIMER_LEFT_49_SEQUENCE=CCGTCAAGGATTTTTCCAAGT
PRIMER_RIGHT_49_SEQUENCE=GCATCGAAGTTGTCTTTTTC
PRIMER_LEFT_49=652,21
PRIMER_RIGHT_49=1548,20
PRIMER_LEFT_49_TM=60.338
PRIMER_RIGHT_49_TM=55.075
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=40.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=4.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.7000
PRIMER_RIGHT_49_END_STABILITY=7.3000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=897
=
SEQUENCE_ID=MAGIC.149_1_12762161_12762257_wil-2_kn-0_11382_249415_TRUE
SEQUENCE_TARGET=950,196
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNTNNNNNNNANNANANGTTATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNGNAAAATTCATTTACTCTNNTAANTTGGTTACTCNATGNNNNNNGATTATGCTANTNTGTAGGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNANTAGCCACGTNGCTTTAGACCATTTTGTAGTTNTTATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_15990190_15990358_tsu-0_wil-2_1686_752143_TRUE
SEQUENCE_TARGET=950,268
SEQUENCE_TEMPLATE=ANGGTAATTCNTTTTTTTTATTCAATGCAANTTGATGANGATGATATGATCACTAATNTATTNTGGGNAGATNATCGGTCTATAAGTGATTACAATCNTTTTGGAGATGTCATTTGCTTTGACACAACTTATAAGACCAATGAATATGATAGANCCTTTGCTCCNTTTATCGGGGTCAATCATCACANGNAAACAGTTGTGTTNNGNGCTGCTCTCTTATATGATGAAACCAGTGAGTCATATAAGTGGNTTTTCGAGACTNTTCTTGGAGCAATTTCTNGAAANCAANCAAAAACAATTNTTACAGACCAATGTGCNNCAATGNCNAATACAATAGTAANTNNNNTNCCTGANACAAAANATAGATTATGTGTTNGACATATTTANCAAAATGATGCNAAGAAGTTGAGTCATGTANTTCNTGGAGCNNAACAATTTGCCATNGATTTTGGTAAGTGTGTATATNANCATGAGGAANAAGAAGACTNGTTATTGGCATGGAGTAATATGNTCCAGAAGCATAACTTGANANATGATAAATNNTTGNAGANTTTGTTTGANCTGAGAGAAAAATGTGCAATGGTATATGGACGTCATACTTTTACAGNAGATATNGTGAGCACACAACGTAGTAAAAGTATGAATAATATTTTGNAAAAAATACTTGAAGCATAGTTATAACTTNTTGTGCTTCTCTGAACACTNCGAGAGAGTGTTGGAAGATCNACGATATAAGGNATTAGTTGNTGATTTCAATATGATGCATACCTCGCCGGTATTATNTGCTACTNTAGAGATGTTGGAGATNTTGCAANATGCAGAGGAAGTGTATANACCCNAAATTTNTAGCTTGTTCNAGAAACAATACATAGTTATTGGTGATTATGTTGCCAACAAGGTTAGTAAGTCTGAGATGGTATATAAATATAAAGTATCTTATNGTGGTGTATNANNNGAGCATTTGGTTAATTATGATGTTNNAAACNAGACGATATGTTNTNGTTGCATGAAGTTTAGNTTTGCTGNGATTTTATGTCGTCATGCATNGAAANTNTTGGACAAGAAGAATGTTAGAAGAATCNCATNTANNTACATTCTAAATNGATGGAGTAAAGAGNCAAAAGCNCNAAAAATATCTTCTTANCATTCAGAGTCACCTGATGAGANANTCAAGCAATCGATTGGAAANCNATATAGCCATATANGTNGTACTTTCCNTGAGACTNCATNTGTTGCTGATGATCATATAGAATTGACNTTGTGNNCAAAATGAAGATNCCATTATGTTGCTNAAAAAATTNGAGGNAAAAANGAGCTTTTAAAATNTAATNCATGGATNCTCCAAACTTGAGATGTTGAANTTGTGGAAGAAGAANAAGAAGANGAAGAAGANAAANANAAGGATGTTTNAAATGCACNNGGGNTAAANANAAAGNTCNTNTTGGACGACCAAAANANAAAAAANTCGGANCCCANGNTCGTTTTATGAGNGTTCTTGAAANNNAAAANTCNGNGTACNTCAAAACAATNAAGTAATGCAACAACAAAGAAAAAGTTATTATTTNAGGTACTNGATANNATATATNTCATATTAGTNTATGTCTAATTTTCATNAAAATGNGANTTAATCTATTTATACTAACTNTTANTTGAAATNGCTAAATNCTTCAGNACTCTACCCANTTATGAGNCGTAACCACCAANCCGANCTTACAAAATNGATCNTTTTTTNNACAANTGTTCNANGTAAATACTTAANTTCTTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.426_2_2925433_2925696_can-0_oy-0_788_168128_TRUE
SEQUENCE_TARGET=950,363
SEQUENCE_TEMPLATE=CTTTCACTCTATTCGATGTATTGTACACATCTGGCACANNNNNNNNNNNNTGTCTTCTTCTTCGATCCTCGNTTTCATCCACANTATACAANNGNNAAATTTCCGATTNTCTCCTCACTGNNTCTCTAGAATCCATCNTNGATTCAAACNCATTTTCCGATCAAACGNCATNTTNTTCCCTNTCGAGNGAGNGATAATGTGGAAACTGAAGATCGCGGAAGGAGGTAGTCCATGGCTTAGAACCACCAATAATCACGTCGGAAGACAGTTTTGGGAGTTCGATCCGAATCTCGGTNNTCCTGANGATCTNGCCGCCGTCGANGAAGCTAGGAAGTCTTTTTCNGANAATCGATTCGTGCAGAAACATAGCGCCGATCTGCTTATGCGNCTTCAGGTATTATCTCTNNCTTCTCTTTGTTTCCNTTGACTTTAGNGTGGTNTTGACTGGTGACGAATCTCGCAAGTTTTAGTCAGTGATACTTAGGACTGTGTTGCTTCATCNNANNNNNNNNNNNNNNNTNCANCNCTNGNNNTNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGTGAACTTAGCTNGTAACTAGATTTGATTGTATCTTACTNCATGGATATGNANGAGANNNNNNNNTACAATAGATGGNAAAGTACANAGAGCATATACAACNNCAAAANCTAGAGTTAATNNANNNTNNNNNNNTTNTCNNNNNNNNTNANAANNNAATNNCNATNTNGNGNNNNTANNANNNNNNNNNGANTCNNNTANNGNTTNATTTNCANCNNNNNNNNNNNNNTNNNNNNNNNGNATTTNGTCNNAAGNTGANNNCNNAANATNTNNNNNAACNTANNNCTANNATTNTTTNANNGNTTNTNTTTACNACNNNTTGTNNNCNNTTNTNTNTCTANAGNNTNTNANNATNTGNNTTNNNTTANTNNTANNNNNNNNNNNNNNNNTNNCTTNCTNNTNNANTNTNTNNTNTTNNNNCAGNNNNTNATNTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTCTCTCTTTCTCTNTNTGTAGTTTTCAAGAGAAAATTTGATTANCCCAGTTNTACCTCAAGTCAAAATCGAAGACACTGATGATGTTACAGAGGAGNTGGTGGAAACNACGTTAAAGAGGGGTCTAGATTTCTATTCAACTATACAGGCACACGANGGGCACTGGCCAGGTGATTATGGTGGTCCTATGTTTCTTCTCCCAGGACTGGTCAGTTCATATNTTTCGTGCCTAAGCTTTCTTTTGNAAATTTTACNGAAAACTTGACAAAAACTANTTNTGATNACAGATAATTACACTCTCCATAACTGGAGCACTGAATACAGTATTGTCGGAACAACATAAACAAGAAATGCGCCGTTATCTNTATAATCACCAGGNAAGAAATTTCTCNNATCTTTACTATAANTCTGTTTCCTTGCAATGCTNTACTTGGANTTTCCATCNCAAAATAGATGTTTACACATCTTTNTGTTTCAAANANTTTCNTCTTCTACTTTCNAATTGTTAAACTAATTTGCACTATTGGNGGCATGTGGTTTCAGAATGAGGACGGAGGTTGGGGTTTACATATTGAGGGCCCNAGCACCATGTTTGGGTCTGTGTTGAACTATGTTACTCTAAGGTTGCTTGGAGAAGGNCCTAANGATGGNGANGGAGATATGGAGAAAGGACGAGACTGGATACTAAATCATGGTGGTGCTACCAATATTACATCTTGGGGGAAAATGTGGCTATCGGTACTAAANTTTTGACCTCTATCTGTCCTTTAAAGTTATCGTATGNNAATTTCTGTTACTTGACTNATCTTTTTGCTTGGATCTGCTNATACTGTNAATAAAGGTACTTGGAGCTTTTGAATGGNCNGGAAATAACCCACTGCCACCTGAGATATGGCTTNTCCCATATTTCCTGCCAATNCATCCAGGTAAAATGACAATNTCATGTNTTCTTGACNTTCTCAACTTANATACTGTTTAANTTTATTCTTTTCNCNGTCTCCNATTGTTGATNNGACTAAACTATTCATTCNAATGAAGTGCCTCGTATTATCAATTCTTGGTTCAGNCGCTATTTATANAGNATGGAAAANCNANNN
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=2.636485
PRIMER_LEFT_0_PENALTY=2.464167
PRIMER_RIGHT_0_PENALTY=0.172318
PRIMER_LEFT_0_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_0_SEQUENCE=CTGACCAGTCCTGGGAGAAG
PRIMER_LEFT_0=451,22
PRIMER_RIGHT_0=1378,20
PRIMER_LEFT_0_TM=59.536
PRIMER_RIGHT_0_TM=59.828
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=60.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=928
PRIMER_PAIR_1_PENALTY=3.236264
PRIMER_LEFT_1_PENALTY=2.464167
PRIMER_RIGHT_1_PENALTY=0.772097
PRIMER_LEFT_1_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_1_SEQUENCE=GACCAGTCCTGGGAGAAGAA
PRIMER_LEFT_1=451,22
PRIMER_RIGHT_1=1376,20
PRIMER_LEFT_1_TM=59.536
PRIMER_RIGHT_1_TM=59.228
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=926
PRIMER_PAIR_2_PENALTY=3.259552
PRIMER_LEFT_2_PENALTY=2.464167
PRIMER_RIGHT_2_PENALTY=0.795385
PRIMER_LEFT_2_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_2_SEQUENCE=TGACCAGTCCTGGGAGAAGA
PRIMER_LEFT_2=451,22
PRIMER_RIGHT_2=1377,20
PRIMER_LEFT_2_TM=59.536
PRIMER_RIGHT_2_TM=60.795
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=927
PRIMER_PAIR_3_PENALTY=3.748696
PRIMER_LEFT_3_PENALTY=2.464167
PRIMER_RIGHT_3_PENALTY=1.284530
PRIMER_LEFT_3_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_3_SEQUENCE=CCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_3=451,22
PRIMER_RIGHT_3=1374,20
PRIMER_LEFT_3_TM=59.536
PRIMER_RIGHT_3_TM=58.715
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PAIR_3_PRODUCT_SIZE=924
PRIMER_PAIR_4_PENALTY=3.748696
PRIMER_LEFT_4_PENALTY=2.464167
PRIMER_RIGHT_4_PENALTY=1.284530
PRIMER_LEFT_4_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_4_SEQUENCE=ACCAGTCCTGGGAGAAGAAA
PRIMER_LEFT_4=451,22
PRIMER_RIGHT_4=1375,20
PRIMER_LEFT_4_TM=59.536
PRIMER_RIGHT_4_TM=58.715
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=925
PRIMER_PAIR_5_PENALTY=3.808908
PRIMER_LEFT_5_PENALTY=2.464167
PRIMER_RIGHT_5_PENALTY=1.344741
PRIMER_LEFT_5_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_5_SEQUENCE=CAAAAGAAAGCTTAGGCACGA
PRIMER_LEFT_5=451,22
PRIMER_RIGHT_5=1409,21
PRIMER_LEFT_5_TM=59.536
PRIMER_RIGHT_5_TM=59.655
PRIMER_LEFT_5_GC_PERCENT=40.909
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=8.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=959
PRIMER_PAIR_6_PENALTY=3.873989
PRIMER_LEFT_6_PENALTY=2.464167
PRIMER_RIGHT_6_PENALTY=1.409822
PRIMER_LEFT_6_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_6_SEQUENCE=ACCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_6=451,22
PRIMER_RIGHT_6=1375,21
PRIMER_LEFT_6_TM=59.536
PRIMER_RIGHT_6_TM=59.590
PRIMER_LEFT_6_GC_PERCENT=40.909
PRIMER_RIGHT_6_GC_PERCENT=52.381
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PAIR_6_PRODUCT_SIZE=925
PRIMER_PAIR_7_PENALTY=4.077310
PRIMER_LEFT_7_PENALTY=2.464167
PRIMER_RIGHT_7_PENALTY=1.613143
PRIMER_LEFT_7_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_7_SEQUENCE=GACCAGTCCTGGGAGAAGAAA
PRIMER_LEFT_7=451,22
PRIMER_RIGHT_7=1376,21
PRIMER_LEFT_7_TM=59.536
PRIMER_RIGHT_7_TM=60.613
PRIMER_LEFT_7_GC_PERCENT=40.909
PRIMER_RIGHT_7_GC_PERCENT=52.381
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=926
PRIMER_PAIR_8_PENALTY=4.150750
PRIMER_LEFT_8_PENALTY=2.464167
PRIMER_RIGHT_8_PENALTY=1.686583
PRIMER_LEFT_8_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_8_SEQUENCE=ACTGACCAGTCCTGGGAGAAG
PRIMER_LEFT_8=451,22
PRIMER_RIGHT_8=1379,21
PRIMER_LEFT_8_TM=59.536
PRIMER_RIGHT_8_TM=60.687
PRIMER_LEFT_8_GC_PERCENT=40.909
PRIMER_RIGHT_8_GC_PERCENT=57.143
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=929
PRIMER_PAIR_9_PENALTY=4.191571
PRIMER_LEFT_9_PENALTY=3.419474
PRIMER_RIGHT_9_PENALTY=0.772097
PRIMER_LEFT_9_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_9_SEQUENCE=GACCAGTCCTGGGAGAAGAA
PRIMER_LEFT_9=451,23
PRIMER_RIGHT_9=1376,20
PRIMER_LEFT_9_TM=60.419
PRIMER_RIGHT_9_TM=59.228
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=926
PRIMER_PAIR_10_PENALTY=4.345433
PRIMER_LEFT_10_PENALTY=4.173115
PRIMER_RIGHT_10_PENALTY=0.172318
PRIMER_LEFT_10_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_10_SEQUENCE=CTGACCAGTCCTGGGAGAAG
PRIMER_LEFT_10=451,21
PRIMER_RIGHT_10=1378,20
PRIMER_LEFT_10_TM=56.827
PRIMER_RIGHT_10_TM=59.828
PRIMER_LEFT_10_GC_PERCENT=42.857
PRIMER_RIGHT_10_GC_PERCENT=60.000
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=5.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PAIR_10_PRODUCT_SIZE=928
PRIMER_PAIR_11_PENALTY=4.458935
PRIMER_LEFT_11_PENALTY=4.286617
PRIMER_RIGHT_11_PENALTY=0.172318
PRIMER_LEFT_11_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_11_SEQUENCE=CTGACCAGTCCTGGGAGAAG
PRIMER_LEFT_11=450,22
PRIMER_RIGHT_11=1378,20
PRIMER_LEFT_11_TM=57.713
PRIMER_RIGHT_11_TM=59.828
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=60.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=5.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PAIR_11_PRODUCT_SIZE=929
PRIMER_PAIR_12_PENALTY=4.491026
PRIMER_LEFT_12_PENALTY=4.173115
PRIMER_RIGHT_12_PENALTY=0.317911
PRIMER_LEFT_12_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_12_SEQUENCE=ACTGACCAGTCCTGGGAGAA
PRIMER_LEFT_12=451,21
PRIMER_RIGHT_12=1379,20
PRIMER_LEFT_12_TM=56.827
PRIMER_RIGHT_12_TM=59.682
PRIMER_LEFT_12_GC_PERCENT=42.857
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_END_STABILITY=5.4000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=929
PRIMER_PAIR_13_PENALTY=4.531683
PRIMER_LEFT_13_PENALTY=2.464167
PRIMER_RIGHT_13_PENALTY=2.067516
PRIMER_LEFT_13_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_13_SEQUENCE=TCCTGGGAGAAGAAACATAGGA
PRIMER_LEFT_13=451,22
PRIMER_RIGHT_13=1370,22
PRIMER_LEFT_13_TM=59.536
PRIMER_RIGHT_13_TM=60.068
PRIMER_LEFT_13_GC_PERCENT=40.909
PRIMER_RIGHT_13_GC_PERCENT=45.455
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=920
PRIMER_PAIR_14_PENALTY=4.604528
PRIMER_LEFT_14_PENALTY=4.286617
PRIMER_RIGHT_14_PENALTY=0.317911
PRIMER_LEFT_14_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_14_SEQUENCE=ACTGACCAGTCCTGGGAGAA
PRIMER_LEFT_14=450,22
PRIMER_RIGHT_14=1379,20
PRIMER_LEFT_14_TM=57.713
PRIMER_RIGHT_14_TM=59.682
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=5.4000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PAIR_14_PRODUCT_SIZE=930
PRIMER_PAIR_15_PENALTY=4.622381
PRIMER_LEFT_15_PENALTY=2.464167
PRIMER_RIGHT_15_PENALTY=2.158214
PRIMER_LEFT_15_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_15_SEQUENCE=CAGTCCTGGGAGAAGAAACA
PRIMER_LEFT_15=451,22
PRIMER_RIGHT_15=1373,20
PRIMER_LEFT_15_TM=59.536
PRIMER_RIGHT_15_TM=57.842
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=923
PRIMER_PAIR_16_PENALTY=4.704004
PRIMER_LEFT_16_PENALTY=3.419474
PRIMER_RIGHT_16_PENALTY=1.284530
PRIMER_LEFT_16_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_16_SEQUENCE=CCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_16=451,23
PRIMER_RIGHT_16=1374,20
PRIMER_LEFT_16_TM=60.419
PRIMER_RIGHT_16_TM=58.715
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PAIR_16_PRODUCT_SIZE=924
PRIMER_PAIR_17_PENALTY=4.704004
PRIMER_LEFT_17_PENALTY=3.419474
PRIMER_RIGHT_17_PENALTY=1.284530
PRIMER_LEFT_17_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_17_SEQUENCE=ACCAGTCCTGGGAGAAGAAA
PRIMER_LEFT_17=451,23
PRIMER_RIGHT_17=1375,20
PRIMER_LEFT_17_TM=60.419
PRIMER_RIGHT_17_TM=58.715
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=925
PRIMER_PAIR_18_PENALTY=4.764215
PRIMER_LEFT_18_PENALTY=3.419474
PRIMER_RIGHT_18_PENALTY=1.344741
PRIMER_LEFT_18_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_18_SEQUENCE=CAAAAGAAAGCTTAGGCACGA
PRIMER_LEFT_18=451,23
PRIMER_RIGHT_18=1409,21
PRIMER_LEFT_18_TM=60.419
PRIMER_RIGHT_18_TM=59.655
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=42.857
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=959
PRIMER_PAIR_19_PENALTY=4.829296
PRIMER_LEFT_19_PENALTY=3.419474
PRIMER_RIGHT_19_PENALTY=1.409822
PRIMER_LEFT_19_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_19_SEQUENCE=ACCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_19=451,23
PRIMER_RIGHT_19=1375,21
PRIMER_LEFT_19_TM=60.419
PRIMER_RIGHT_19_TM=59.590
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=52.381
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=925
PRIMER_PAIR_20_PENALTY=4.945213
PRIMER_LEFT_20_PENALTY=4.173115
PRIMER_RIGHT_20_PENALTY=0.772097
PRIMER_LEFT_20_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_20_SEQUENCE=GACCAGTCCTGGGAGAAGAA
PRIMER_LEFT_20=451,21
PRIMER_RIGHT_20=1376,20
PRIMER_LEFT_20_TM=56.827
PRIMER_RIGHT_20_TM=59.228
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=6.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=5.4000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=2.00
PRIMER_PAIR_20_PRODUCT_SIZE=926
PRIMER_PAIR_21_PENALTY=4.968501
PRIMER_LEFT_21_PENALTY=4.173115
PRIMER_RIGHT_21_PENALTY=0.795385
PRIMER_LEFT_21_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_21_SEQUENCE=TGACCAGTCCTGGGAGAAGA
PRIMER_LEFT_21=451,21
PRIMER_RIGHT_21=1377,20
PRIMER_LEFT_21_TM=56.827
PRIMER_RIGHT_21_TM=60.795
PRIMER_LEFT_21_GC_PERCENT=42.857
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=6.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=5.4000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=927
PRIMER_PAIR_22_PENALTY=5.025361
PRIMER_LEFT_22_PENALTY=2.464167
PRIMER_RIGHT_22_PENALTY=2.561194
PRIMER_LEFT_22_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_22_SEQUENCE=CCAGTCCTGGGAGAAGAAACA
PRIMER_LEFT_22=451,22
PRIMER_RIGHT_22=1374,21
PRIMER_LEFT_22_TM=59.536
PRIMER_RIGHT_22_TM=61.561
PRIMER_LEFT_22_GC_PERCENT=40.909
PRIMER_RIGHT_22_GC_PERCENT=52.381
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=7.0000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=924
PRIMER_PAIR_23_PENALTY=5.032617
PRIMER_LEFT_23_PENALTY=3.419474
PRIMER_RIGHT_23_PENALTY=1.613143
PRIMER_LEFT_23_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_23_SEQUENCE=GACCAGTCCTGGGAGAAGAAA
PRIMER_LEFT_23=451,23
PRIMER_RIGHT_23=1376,21
PRIMER_LEFT_23_TM=60.419
PRIMER_RIGHT_23_TM=60.613
PRIMER_LEFT_23_GC_PERCENT=43.478
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=6.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=926
PRIMER_PAIR_24_PENALTY=5.058714
PRIMER_LEFT_24_PENALTY=4.286617
PRIMER_RIGHT_24_PENALTY=0.772097
PRIMER_LEFT_24_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_24_SEQUENCE=GACCAGTCCTGGGAGAAGAA
PRIMER_LEFT_24=450,22
PRIMER_RIGHT_24=1376,20
PRIMER_LEFT_24_TM=57.713
PRIMER_RIGHT_24_TM=59.228
PRIMER_LEFT_24_GC_PERCENT=40.909
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=5.4000
PRIMER_RIGHT_24_END_STABILITY=7.0000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=927
PRIMER_PAIR_25_PENALTY=5.082002
PRIMER_LEFT_25_PENALTY=4.286617
PRIMER_RIGHT_25_PENALTY=0.795385
PRIMER_LEFT_25_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_25_SEQUENCE=TGACCAGTCCTGGGAGAAGA
PRIMER_LEFT_25=450,22
PRIMER_RIGHT_25=1377,20
PRIMER_LEFT_25_TM=57.713
PRIMER_RIGHT_25_TM=60.795
PRIMER_LEFT_25_GC_PERCENT=40.909
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=6.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=5.4000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=928
PRIMER_PAIR_26_PENALTY=5.210935
PRIMER_LEFT_26_PENALTY=2.464167
PRIMER_RIGHT_26_PENALTY=2.746768
PRIMER_LEFT_26_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_26_SEQUENCE=CCTGGGAGAAGAAACATAGGA
PRIMER_LEFT_26=451,22
PRIMER_RIGHT_26=1369,21
PRIMER_LEFT_26_TM=59.536
PRIMER_RIGHT_26_TM=58.253
PRIMER_LEFT_26_GC_PERCENT=40.909
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=6.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=7.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=2.00
PRIMER_PAIR_26_PRODUCT_SIZE=919
PRIMER_PAIR_27_PENALTY=5.218236
PRIMER_LEFT_27_PENALTY=2.464167
PRIMER_RIGHT_27_PENALTY=2.754069
PRIMER_LEFT_27_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_27_SEQUENCE=CTGACCAGTCCTGGGAGAAGA
PRIMER_LEFT_27=451,22
PRIMER_RIGHT_27=1378,21
PRIMER_LEFT_27_TM=59.536
PRIMER_RIGHT_27_TM=61.754
PRIMER_LEFT_27_GC_PERCENT=40.909
PRIMER_RIGHT_27_GC_PERCENT=57.143
PRIMER_LEFT_27_SELF_ANY=6.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PAIR_27_PRODUCT_SIZE=928
PRIMER_PAIR_28_PENALTY=5.309863
PRIMER_LEFT_28_PENALTY=2.464167
PRIMER_RIGHT_28_PENALTY=2.845696
PRIMER_LEFT_28_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_28_SEQUENCE=TGGGAGAAGAAACATAGGACCA
PRIMER_LEFT_28=451,22
PRIMER_RIGHT_28=1367,22
PRIMER_LEFT_28_TM=59.536
PRIMER_RIGHT_28_TM=60.846
PRIMER_LEFT_28_GC_PERCENT=40.909
PRIMER_RIGHT_28_GC_PERCENT=45.455
PRIMER_LEFT_28_SELF_ANY=6.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PAIR_28_PRODUCT_SIZE=917
PRIMER_PAIR_29_PENALTY=5.356544
PRIMER_LEFT_29_PENALTY=2.464167
PRIMER_RIGHT_29_PENALTY=2.892377
PRIMER_LEFT_29_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_29_SEQUENCE=GGGAGAAGAAACATAGGACCA
PRIMER_LEFT_29=451,22
PRIMER_RIGHT_29=1366,21
PRIMER_LEFT_29_TM=59.536
PRIMER_RIGHT_29_TM=58.108
PRIMER_LEFT_29_GC_PERCENT=40.909
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=6.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PAIR_29_PRODUCT_SIZE=916
PRIMER_PAIR_30_PENALTY=5.363308
PRIMER_LEFT_30_PENALTY=2.464167
PRIMER_RIGHT_30_PENALTY=2.899141
PRIMER_LEFT_30_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_30_SEQUENCE=CCTGGGAGAAGAAACATAGGAC
PRIMER_LEFT_30=451,22
PRIMER_RIGHT_30=1369,22
PRIMER_LEFT_30_TM=59.536
PRIMER_RIGHT_30_TM=59.101
PRIMER_LEFT_30_GC_PERCENT=40.909
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=3.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.6000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=2.00
PRIMER_PAIR_30_PRODUCT_SIZE=919
PRIMER_PAIR_31_PENALTY=5.457645
PRIMER_LEFT_31_PENALTY=4.173115
PRIMER_RIGHT_31_PENALTY=1.284530
PRIMER_LEFT_31_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_31_SEQUENCE=CCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_31=451,21
PRIMER_RIGHT_31=1374,20
PRIMER_LEFT_31_TM=56.827
PRIMER_RIGHT_31_TM=58.715
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=6.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=5.4000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=924
PRIMER_PAIR_32_PENALTY=5.457645
PRIMER_LEFT_32_PENALTY=4.173115
PRIMER_RIGHT_32_PENALTY=1.284530
PRIMER_LEFT_32_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_32_SEQUENCE=ACCAGTCCTGGGAGAAGAAA
PRIMER_LEFT_32=451,21
PRIMER_RIGHT_32=1375,20
PRIMER_LEFT_32_TM=56.827
PRIMER_RIGHT_32_TM=58.715
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=5.4000
PRIMER_RIGHT_32_END_STABILITY=7.0000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=925
PRIMER_PAIR_33_PENALTY=5.486990
PRIMER_LEFT_33_PENALTY=3.419474
PRIMER_RIGHT_33_PENALTY=2.067516
PRIMER_LEFT_33_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_33_SEQUENCE=TCCTGGGAGAAGAAACATAGGA
PRIMER_LEFT_33=451,23
PRIMER_RIGHT_33=1370,22
PRIMER_LEFT_33_TM=60.419
PRIMER_RIGHT_33_TM=60.068
PRIMER_LEFT_33_GC_PERCENT=43.478
PRIMER_RIGHT_33_GC_PERCENT=45.455
PRIMER_LEFT_33_SELF_ANY=6.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.4000
PRIMER_RIGHT_33_END_STABILITY=7.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=920
PRIMER_PAIR_34_PENALTY=5.496998
PRIMER_LEFT_34_PENALTY=2.464167
PRIMER_RIGHT_34_PENALTY=3.032831
PRIMER_LEFT_34_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_34_SEQUENCE=GGAGAAGAAACATAGGACCACC
PRIMER_LEFT_34=451,22
PRIMER_RIGHT_34=1365,22
PRIMER_LEFT_34_TM=59.536
PRIMER_RIGHT_34_TM=58.967
PRIMER_LEFT_34_GC_PERCENT=40.909
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=3.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=915
PRIMER_PAIR_35_PENALTY=5.496998
PRIMER_LEFT_35_PENALTY=2.464167
PRIMER_RIGHT_35_PENALTY=3.032831
PRIMER_LEFT_35_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_35_SEQUENCE=GGGAGAAGAAACATAGGACCAC
PRIMER_LEFT_35=451,22
PRIMER_RIGHT_35=1366,22
PRIMER_LEFT_35_TM=59.536
PRIMER_RIGHT_35_TM=58.967
PRIMER_LEFT_35_GC_PERCENT=40.909
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=916
PRIMER_PAIR_36_PENALTY=5.502004
PRIMER_LEFT_36_PENALTY=2.464167
PRIMER_RIGHT_36_PENALTY=3.037837
PRIMER_LEFT_36_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_36_SEQUENCE=AAGAAAGCTTAGGCACGAAA
PRIMER_LEFT_36=451,22
PRIMER_RIGHT_36=1406,20
PRIMER_LEFT_36_TM=59.536
PRIMER_RIGHT_36_TM=56.962
PRIMER_LEFT_36_GC_PERCENT=40.909
PRIMER_RIGHT_36_GC_PERCENT=40.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=9.0000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=956
PRIMER_PAIR_37_PENALTY=5.502004
PRIMER_LEFT_37_PENALTY=2.464167
PRIMER_RIGHT_37_PENALTY=3.037837
PRIMER_LEFT_37_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_37_SEQUENCE=AAAAGAAAGCTTAGGCACGA
PRIMER_LEFT_37=451,22
PRIMER_RIGHT_37=1408,20
PRIMER_LEFT_37_TM=59.536
PRIMER_RIGHT_37_TM=56.962
PRIMER_LEFT_37_GC_PERCENT=40.909
PRIMER_RIGHT_37_GC_PERCENT=40.000
PRIMER_LEFT_37_SELF_ANY=6.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=8.4000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=958
PRIMER_PAIR_38_PENALTY=5.517856
PRIMER_LEFT_38_PENALTY=4.173115
PRIMER_RIGHT_38_PENALTY=1.344741
PRIMER_LEFT_38_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_38_SEQUENCE=CAAAAGAAAGCTTAGGCACGA
PRIMER_LEFT_38=451,21
PRIMER_RIGHT_38=1409,21
PRIMER_LEFT_38_TM=56.827
PRIMER_RIGHT_38_TM=59.655
PRIMER_LEFT_38_GC_PERCENT=42.857
PRIMER_RIGHT_38_GC_PERCENT=42.857
PRIMER_LEFT_38_SELF_ANY=6.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=5.4000
PRIMER_RIGHT_38_END_STABILITY=8.4000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=959
PRIMER_PAIR_39_PENALTY=5.571146
PRIMER_LEFT_39_PENALTY=4.286617
PRIMER_RIGHT_39_PENALTY=1.284530
PRIMER_LEFT_39_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_39_SEQUENCE=CCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_39=450,22
PRIMER_RIGHT_39=1374,20
PRIMER_LEFT_39_TM=57.713
PRIMER_RIGHT_39_TM=58.715
PRIMER_LEFT_39_GC_PERCENT=40.909
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=6.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=5.4000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=3.00
PRIMER_PAIR_39_PRODUCT_SIZE=925
PRIMER_PAIR_40_PENALTY=5.571146
PRIMER_LEFT_40_PENALTY=4.286617
PRIMER_RIGHT_40_PENALTY=1.284530
PRIMER_LEFT_40_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_40_SEQUENCE=ACCAGTCCTGGGAGAAGAAA
PRIMER_LEFT_40=450,22
PRIMER_RIGHT_40=1375,20
PRIMER_LEFT_40_TM=57.713
PRIMER_RIGHT_40_TM=58.715
PRIMER_LEFT_40_GC_PERCENT=40.909
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=6.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.4000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=926
PRIMER_PAIR_41_PENALTY=5.577688
PRIMER_LEFT_41_PENALTY=3.419474
PRIMER_RIGHT_41_PENALTY=2.158214
PRIMER_LEFT_41_SEQUENCE=CGAATCTCGCAAGTTTTAGTCAG
PRIMER_RIGHT_41_SEQUENCE=CAGTCCTGGGAGAAGAAACA
PRIMER_LEFT_41=451,23
PRIMER_RIGHT_41=1373,20
PRIMER_LEFT_41_TM=60.419
PRIMER_RIGHT_41_TM=57.842
PRIMER_LEFT_41_GC_PERCENT=43.478
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=6.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.4000
PRIMER_RIGHT_41_END_STABILITY=7.0000
PRIMER_PAIR_41_COMPL_ANY=5.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=923
PRIMER_PAIR_42_PENALTY=5.582937
PRIMER_LEFT_42_PENALTY=4.173115
PRIMER_RIGHT_42_PENALTY=1.409822
PRIMER_LEFT_42_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_42_SEQUENCE=ACCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_42=451,21
PRIMER_RIGHT_42=1375,21
PRIMER_LEFT_42_TM=56.827
PRIMER_RIGHT_42_TM=59.590
PRIMER_LEFT_42_GC_PERCENT=42.857
PRIMER_RIGHT_42_GC_PERCENT=52.381
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.4000
PRIMER_RIGHT_42_END_STABILITY=6.7000
PRIMER_PAIR_42_COMPL_ANY=5.00
PRIMER_PAIR_42_COMPL_END=3.00
PRIMER_PAIR_42_PRODUCT_SIZE=925
PRIMER_PAIR_43_PENALTY=5.592499
PRIMER_LEFT_43_PENALTY=2.464167
PRIMER_RIGHT_43_PENALTY=3.128332
PRIMER_LEFT_43_SEQUENCE=CGAATCTCGCAAGTTTTAGTCA
PRIMER_RIGHT_43_SEQUENCE=TGACCAGTCCTGGGAGAAGAA
PRIMER_LEFT_43=451,22
PRIMER_RIGHT_43=1377,21
PRIMER_LEFT_43_TM=59.536
PRIMER_RIGHT_43_TM=62.128
PRIMER_LEFT_43_GC_PERCENT=40.909
PRIMER_RIGHT_43_GC_PERCENT=52.381
PRIMER_LEFT_43_SELF_ANY=6.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=7.0000
PRIMER_PAIR_43_COMPL_ANY=5.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=927
PRIMER_PAIR_44_PENALTY=5.631358
PRIMER_LEFT_44_PENALTY=4.286617
PRIMER_RIGHT_44_PENALTY=1.344741
PRIMER_LEFT_44_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_44_SEQUENCE=CAAAAGAAAGCTTAGGCACGA
PRIMER_LEFT_44=450,22
PRIMER_RIGHT_44=1409,21
PRIMER_LEFT_44_TM=57.713
PRIMER_RIGHT_44_TM=59.655
PRIMER_LEFT_44_GC_PERCENT=40.909
PRIMER_RIGHT_44_GC_PERCENT=42.857
PRIMER_LEFT_44_SELF_ANY=6.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=5.4000
PRIMER_RIGHT_44_END_STABILITY=8.4000
PRIMER_PAIR_44_COMPL_ANY=5.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=960
PRIMER_PAIR_45_PENALTY=5.680543
PRIMER_LEFT_45_PENALTY=5.362632
PRIMER_RIGHT_45_PENALTY=0.317911
PRIMER_LEFT_45_SEQUENCE=CTTAGGACTGTGTTGCTTCATC
PRIMER_RIGHT_45_SEQUENCE=ACTGACCAGTCCTGGGAGAA
PRIMER_LEFT_45=479,22
PRIMER_RIGHT_45=1379,20
PRIMER_LEFT_45_TM=56.637
PRIMER_RIGHT_45_TM=59.682
PRIMER_LEFT_45_GC_PERCENT=45.455
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.6000
PRIMER_RIGHT_45_END_STABILITY=6.7000
PRIMER_PAIR_45_COMPL_ANY=7.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=901
PRIMER_PAIR_46_PENALTY=5.696438
PRIMER_LEFT_46_PENALTY=4.286617
PRIMER_RIGHT_46_PENALTY=1.409822
PRIMER_LEFT_46_SEQUENCE=ACGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_46_SEQUENCE=ACCAGTCCTGGGAGAAGAAAC
PRIMER_LEFT_46=450,22
PRIMER_RIGHT_46=1375,21
PRIMER_LEFT_46_TM=57.713
PRIMER_RIGHT_46_TM=59.590
PRIMER_LEFT_46_GC_PERCENT=40.909
PRIMER_RIGHT_46_GC_PERCENT=52.381
PRIMER_LEFT_46_SELF_ANY=6.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=5.4000
PRIMER_RIGHT_46_END_STABILITY=6.7000
PRIMER_PAIR_46_COMPL_ANY=5.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=926
PRIMER_PAIR_47_PENALTY=5.760336
PRIMER_LEFT_47_PENALTY=5.442425
PRIMER_RIGHT_47_PENALTY=0.317911
PRIMER_LEFT_47_SEQUENCE=ACTTAGGACTGTGTTGCTTCATC
PRIMER_RIGHT_47_SEQUENCE=ACTGACCAGTCCTGGGAGAA
PRIMER_LEFT_47=478,23
PRIMER_RIGHT_47=1379,20
PRIMER_LEFT_47_TM=57.558
PRIMER_RIGHT_47_TM=59.682
PRIMER_LEFT_47_GC_PERCENT=43.478
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=6.00
PRIMER_LEFT_47_SELF_END=1.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.6000
PRIMER_RIGHT_47_END_STABILITY=6.7000
PRIMER_PAIR_47_COMPL_ANY=7.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=902
PRIMER_PAIR_48_PENALTY=5.786258
PRIMER_LEFT_48_PENALTY=4.173115
PRIMER_RIGHT_48_PENALTY=1.613143
PRIMER_LEFT_48_SEQUENCE=CGAATCTCGCAAGTTTTAGTC
PRIMER_RIGHT_48_SEQUENCE=GACCAGTCCTGGGAGAAGAAA
PRIMER_LEFT_48=451,21
PRIMER_RIGHT_48=1376,21
PRIMER_LEFT_48_TM=56.827
PRIMER_RIGHT_48_TM=60.613
PRIMER_LEFT_48_GC_PERCENT=42.857
PRIMER_RIGHT_48_GC_PERCENT=52.381
PRIMER_LEFT_48_SELF_ANY=6.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.4000
PRIMER_RIGHT_48_END_STABILITY=7.0000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=3.00
PRIMER_PAIR_48_PRODUCT_SIZE=926
PRIMER_PAIR_49_PENALTY=5.851584
PRIMER_LEFT_49_PENALTY=5.533673
PRIMER_RIGHT_49_PENALTY=0.317911
PRIMER_LEFT_49_SEQUENCE=TTAGGACTGTGTTGCTTCATC
PRIMER_RIGHT_49_SEQUENCE=ACTGACCAGTCCTGGGAGAA
PRIMER_LEFT_49=480,21
PRIMER_RIGHT_49=1379,20
PRIMER_LEFT_49_TM=55.466
PRIMER_RIGHT_49_TM=59.682
PRIMER_LEFT_49_GC_PERCENT=42.857
PRIMER_RIGHT_49_GC_PERCENT=55.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.6000
PRIMER_RIGHT_49_END_STABILITY=6.7000
PRIMER_PAIR_49_COMPL_ANY=7.00
PRIMER_PAIR_49_COMPL_END=3.00
PRIMER_PAIR_49_PRODUCT_SIZE=900
=
SEQUENCE_ID=MAGIC.329_3_11956936_11957240_tsu-0_ws-0_607_525021_TRUE
SEQUENCE_TARGET=950,404
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNAACGTTCTATAATTTNTATNNCTTTNAATAATTTATTNAATTAATACTTAAATAATAAAGGGTNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTNAANNANATAAATGGTATGTCTTTCACTTTGNACGGAGTATCCCNATGTGGGGTACATAAATATTAAGAGAAAAAAAGAGATCAAGCTTACTAGTTTAAGCGTTAATAATTATACTGACTGACCAATCTACTTGTTCATGTAGCTCTAATGTTATTAATTANTATATAGTCTTAGATTGATCTAATTAATGTCTGTGTATGATGTCACTTTGTGATGATGGAAGTCATGAGAGCTTCCATTACTTAACTCTCTCTCTCTCTNTCTATNTATNTTTACGGTCATGTATAGTGTAATCANNAATAACAAGAAAAAGCCAAGTTCAAAGCACNATACTATTAGTTAAAGACAAATGCGATAATGATACGATTCTGAGCATTACTTGAGTCNCAATATATAGTCACACCCTCATCAAATTCTTTNTATCTTACCAACTTCTNAAAAAAAANNAAAAANNNAAAAGACAAGTAAATACATANATAAATATACACNCACACACACACACATATATTGATATACTTATAATNATAAATCATGGTAAAGTTTGANACAAAACTATGGAAGGCTGTGCTTATGATGTCCATGATCAACATTGGTTTAAGTGTTGTAAACGTTATGTTCAAGAAGATGATTGATGAAGGACTTAACCGTATGGTCGCGACCACTTACCGACTTGCTGTAGGAACTCTGTTCTTNATACCATTCGCGATTTTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNTTNNNNNNNNTNTNNNNNGNTNNNTGTNTTNTTNNTTNTNNNNNTTNNANTTNTNNTTNAANTNTNNNNTTNAATNATAANNGGATGTCTTGTTTATACNANTNTCTAGTTGAATGTTGTTTTCAAACTCTTTTCTCCTGTGAATATGTNATTATATATTCGTATTCCATATGAAAGATTTTGNNNNNNNNNNNNNTTGTTCTTTTTTTNNNNNTCTCTTTTNGTAGACATAACAGGCCAAAACTCACGGGTAGGATCTTGTGTTCACTCTTCTTTAGCGCTCTTCTGGGGTATGTTCATTTTATTAGACTGTTCANTTATAATAAATGTTTACAAATAAGTTTTGAGTTGTTTNTTNTGATCTTGATCGTTNNTATATTTGCAGGACAAGTTTGGTGCAATACTTCTTTCTTATTGGACTAGAGTATACATCTTCAACNTTTTCCTTAGCNTTTAGCAACATGGTTCCTTCNGTTACNTTTGCNTTGGCTCTCGTCTTTAGGTANTNAAAAATCGATCAAACNCATTTGNATTCTTCATATATATNCTCTAGTATTATCNNAATNNTTNATAAACTANANGANTAGGCAAGAGACGCTGAACATCAAGAGCAACGTAGGAAGAGCCAAACTGCTAGGCACAATGATATGTATTTGTGGAGCTTTAGTGCTTACGCTTTATAAAGGAACCGCTTTAAGTCGAGAACANAGTACTCATATGNAAACACATACAAGAACTGATTCCACCGGTGCAATGACNCAAAAGTGGGCAATGGGTTCGATCATGCTGGTTATATCNATCATNATATGGTCATCTTGGTTCATTGTTCAAGCTAAAATAAGCNGANTTTATCCNTGCCAATACACGAGTACCACGATCCTCTCTTTCTTTGGCGTGATNCAATCTGCTTTGTTGAGTTTGATCTCGGAGAGGAGCACNTCTATGTGGGTCGTTAAAGACAAGTTCCAAGTTTTAGCTTTACTTTATTCGGTGAGTNATCCAATATACTATTTAATCAACCTACAAGATCTATATGTCAANATATATANAATTTTNTTTNAGNNNNNNNTTGTAAANAAAGNNAAAAAGAAANAAANAACNATTTATGTTTGCATTTGANATGGNTNTTTTCTTTCTTGTCCCCCAATGACTTGAGTTCAACTTTTTTCATGTCCCATATCTATCNTTTTTGCTATNTTTTCTTCTNNGNTAANNTTANTTTNCNAATCCACCACNGGTGTAGGGTATCNTTGGATCGGGATTGTGCTACGTGGGAATGTCGTGGTGTCTCCGNCAAAGAGGTGCGGTNTTCACGTCGAGCTTCATACCNTTGATTCAAGTCTTTGCTGCCATTTTCAGCTTCTCTTTCCTT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.327749
PRIMER_LEFT_0_PENALTY=0.312496
PRIMER_RIGHT_0_PENALTY=0.015253
PRIMER_LEFT_0_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_0_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_0=814,20
PRIMER_RIGHT_0=1560,20
PRIMER_LEFT_0_TM=60.312
PRIMER_RIGHT_0_TM=60.015
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_LEFT_0_SELF_END=1.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=8.5000
PRIMER_RIGHT_0_END_STABILITY=8.5000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=747
PRIMER_PAIR_1_PENALTY=0.332263
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=0.019767
PRIMER_LEFT_1_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_1_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_1=814,20
PRIMER_RIGHT_1=1536,20
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=723
PRIMER_PAIR_2_PENALTY=0.346176
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.033680
PRIMER_LEFT_2_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_2_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_2=814,20
PRIMER_RIGHT_2=1680,20
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=867
PRIMER_PAIR_3_PENALTY=0.346176
PRIMER_LEFT_3_PENALTY=0.312496
PRIMER_RIGHT_3_PENALTY=0.033680
PRIMER_LEFT_3_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_3_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_3=814,20
PRIMER_RIGHT_3=1681,20
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=868
PRIMER_PAIR_4_PENALTY=0.466519
PRIMER_LEFT_4_PENALTY=0.312496
PRIMER_RIGHT_4_PENALTY=0.154023
PRIMER_LEFT_4_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_4_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_4=814,20
PRIMER_RIGHT_4=1571,20
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PAIR_4_PRODUCT_SIZE=758
PRIMER_PAIR_5_PENALTY=0.668069
PRIMER_LEFT_5_PENALTY=0.312496
PRIMER_RIGHT_5_PENALTY=0.355573
PRIMER_LEFT_5_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_5_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_5=814,20
PRIMER_RIGHT_5=1572,20
PRIMER_LEFT_5_TM=60.312
PRIMER_RIGHT_5_TM=59.644
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=759
PRIMER_PAIR_6_PENALTY=0.716170
PRIMER_LEFT_6_PENALTY=0.312496
PRIMER_RIGHT_6_PENALTY=0.403673
PRIMER_LEFT_6_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_6_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_6=814,20
PRIMER_RIGHT_6=1545,20
PRIMER_LEFT_6_TM=60.312
PRIMER_RIGHT_6_TM=59.596
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=732
PRIMER_PAIR_7_PENALTY=0.850580
PRIMER_LEFT_7_PENALTY=0.312496
PRIMER_RIGHT_7_PENALTY=0.538083
PRIMER_LEFT_7_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_7_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_7=814,20
PRIMER_RIGHT_7=1559,20
PRIMER_LEFT_7_TM=60.312
PRIMER_RIGHT_7_TM=60.538
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=746
PRIMER_PAIR_8_PENALTY=1.090076
PRIMER_LEFT_8_PENALTY=0.312496
PRIMER_RIGHT_8_PENALTY=0.777580
PRIMER_LEFT_8_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_8_SEQUENCE=GCCTAGCAGTTTGGCTCTTC
PRIMER_LEFT_8=814,20
PRIMER_RIGHT_8=1570,20
PRIMER_LEFT_8_TM=60.312
PRIMER_RIGHT_8_TM=59.222
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=757
PRIMER_PAIR_9_PENALTY=1.176120
PRIMER_LEFT_9_PENALTY=0.312496
PRIMER_RIGHT_9_PENALTY=0.863624
PRIMER_LEFT_9_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_9_SEQUENCE=TAACCAGCATGATCGAACCC
PRIMER_LEFT_9=814,20
PRIMER_RIGHT_9=1724,20
PRIMER_LEFT_9_TM=60.312
PRIMER_RIGHT_9_TM=60.864
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=911
PRIMER_PAIR_10_PENALTY=1.235854
PRIMER_LEFT_10_PENALTY=0.312496
PRIMER_RIGHT_10_PENALTY=0.923358
PRIMER_LEFT_10_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_10_SEQUENCE=CCTAGCAGTTTGGCTCTTCC
PRIMER_LEFT_10=814,20
PRIMER_RIGHT_10=1569,20
PRIMER_LEFT_10_TM=60.312
PRIMER_RIGHT_10_TM=59.077
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=756
PRIMER_PAIR_11_PENALTY=1.246241
PRIMER_LEFT_11_PENALTY=1.230989
PRIMER_RIGHT_11_PENALTY=0.015253
PRIMER_LEFT_11_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_11_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_11=853,20
PRIMER_RIGHT_11=1560,20
PRIMER_LEFT_11_TM=58.769
PRIMER_RIGHT_11_TM=60.015
PRIMER_LEFT_11_GC_PERCENT=40.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=708
PRIMER_PAIR_12_PENALTY=1.250755
PRIMER_LEFT_12_PENALTY=1.230989
PRIMER_RIGHT_12_PENALTY=0.019767
PRIMER_LEFT_12_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_12_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_12=853,20
PRIMER_RIGHT_12=1536,20
PRIMER_LEFT_12_TM=58.769
PRIMER_RIGHT_12_TM=60.020
PRIMER_LEFT_12_GC_PERCENT=40.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=684
PRIMER_PAIR_13_PENALTY=1.264669
PRIMER_LEFT_13_PENALTY=1.230989
PRIMER_RIGHT_13_PENALTY=0.033680
PRIMER_LEFT_13_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_13_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_13=853,20
PRIMER_RIGHT_13=1680,20
PRIMER_LEFT_13_TM=58.769
PRIMER_RIGHT_13_TM=59.966
PRIMER_LEFT_13_GC_PERCENT=40.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=828
PRIMER_PAIR_14_PENALTY=1.264669
PRIMER_LEFT_14_PENALTY=1.230989
PRIMER_RIGHT_14_PENALTY=0.033680
PRIMER_LEFT_14_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_14_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_14=853,20
PRIMER_RIGHT_14=1681,20
PRIMER_LEFT_14_TM=58.769
PRIMER_RIGHT_14_TM=59.966
PRIMER_LEFT_14_GC_PERCENT=40.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=829
PRIMER_PAIR_15_PENALTY=1.268471
PRIMER_LEFT_15_PENALTY=0.312496
PRIMER_RIGHT_15_PENALTY=0.955975
PRIMER_LEFT_15_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_15_SEQUENCE=GATGTTCAGCGTCTCTTGCC
PRIMER_LEFT_15=814,20
PRIMER_RIGHT_15=1537,20
PRIMER_LEFT_15_TM=60.312
PRIMER_RIGHT_15_TM=60.956
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=10.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=724
PRIMER_PAIR_16_PENALTY=1.338503
PRIMER_LEFT_16_PENALTY=0.312496
PRIMER_RIGHT_16_PENALTY=1.026007
PRIMER_LEFT_16_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_16_SEQUENCE=CCTAGCAGTTTGGCTCTTCCT
PRIMER_LEFT_16=814,20
PRIMER_RIGHT_16=1569,21
PRIMER_LEFT_16_TM=60.312
PRIMER_RIGHT_16_TM=60.026
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=756
PRIMER_PAIR_17_PENALTY=1.339574
PRIMER_LEFT_17_PENALTY=0.312496
PRIMER_RIGHT_17_PENALTY=1.027078
PRIMER_LEFT_17_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_17_SEQUENCE=TCGACTTAAAGCGGTTCCTT
PRIMER_LEFT_17=814,20
PRIMER_RIGHT_17=1633,20
PRIMER_LEFT_17_TM=60.312
PRIMER_RIGHT_17_TM=58.973
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=820
PRIMER_PAIR_18_PENALTY=1.345240
PRIMER_LEFT_18_PENALTY=0.312496
PRIMER_RIGHT_18_PENALTY=1.032744
PRIMER_LEFT_18_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_18_SEQUENCE=GGCTCTTCCTACGTTGCTCTT
PRIMER_LEFT_18=814,20
PRIMER_RIGHT_18=1558,21
PRIMER_LEFT_18_TM=60.312
PRIMER_RIGHT_18_TM=60.033
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=52.381
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=745
PRIMER_PAIR_19_PENALTY=1.385011
PRIMER_LEFT_19_PENALTY=1.230989
PRIMER_RIGHT_19_PENALTY=0.154023
PRIMER_LEFT_19_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_19_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_19=853,20
PRIMER_RIGHT_19=1571,20
PRIMER_LEFT_19_TM=58.769
PRIMER_RIGHT_19_TM=60.154
PRIMER_LEFT_19_GC_PERCENT=40.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=719
PRIMER_PAIR_20_PENALTY=1.412921
PRIMER_LEFT_20_PENALTY=0.312496
PRIMER_RIGHT_20_PENALTY=1.100425
PRIMER_LEFT_20_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_20_SEQUENCE=CTCGACTTAAAGCGGTTCCTT
PRIMER_LEFT_20=814,20
PRIMER_RIGHT_20=1634,21
PRIMER_LEFT_20_TM=60.312
PRIMER_RIGHT_20_TM=59.900
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=47.619
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=8.5000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=821
PRIMER_PAIR_21_PENALTY=1.469256
PRIMER_LEFT_21_PENALTY=0.312496
PRIMER_RIGHT_21_PENALTY=1.156760
PRIMER_LEFT_21_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_21_SEQUENCE=GCTCTTGATGTTCAGCGTCTC
PRIMER_LEFT_21=814,20
PRIMER_RIGHT_21=1543,21
PRIMER_LEFT_21_TM=60.312
PRIMER_RIGHT_21_TM=60.157
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=52.381
PRIMER_LEFT_21_SELF_ANY=2.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=1.00
PRIMER_LEFT_21_END_STABILITY=8.5000
PRIMER_RIGHT_21_END_STABILITY=6.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=2.00
PRIMER_PAIR_21_PRODUCT_SIZE=730
PRIMER_PAIR_22_PENALTY=1.586561
PRIMER_LEFT_22_PENALTY=1.230989
PRIMER_RIGHT_22_PENALTY=0.355573
PRIMER_LEFT_22_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_22_SEQUENCE=GTGCCTAGCAGTTTGGCTCT
PRIMER_LEFT_22=853,20
PRIMER_RIGHT_22=1572,20
PRIMER_LEFT_22_TM=58.769
PRIMER_RIGHT_22_TM=59.644
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=6.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=7.0000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=720
PRIMER_PAIR_23_PENALTY=1.615228
PRIMER_LEFT_23_PENALTY=0.312496
PRIMER_RIGHT_23_PENALTY=1.302732
PRIMER_LEFT_23_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_23_SEQUENCE=GGCTCTTCCTACGTTGCTCT
PRIMER_LEFT_23=814,20
PRIMER_RIGHT_23=1558,20
PRIMER_LEFT_23_TM=60.312
PRIMER_RIGHT_23_TM=58.697
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=8.5000
PRIMER_RIGHT_23_END_STABILITY=7.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PAIR_23_PRODUCT_SIZE=745
PRIMER_PAIR_24_PENALTY=1.634662
PRIMER_LEFT_24_PENALTY=1.230989
PRIMER_RIGHT_24_PENALTY=0.403673
PRIMER_LEFT_24_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_24_SEQUENCE=TTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_24=853,20
PRIMER_RIGHT_24=1545,20
PRIMER_LEFT_24_TM=58.769
PRIMER_RIGHT_24_TM=59.596
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=6.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.0000
PRIMER_RIGHT_24_END_STABILITY=9.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=693
PRIMER_PAIR_25_PENALTY=1.644931
PRIMER_LEFT_25_PENALTY=1.629679
PRIMER_RIGHT_25_PENALTY=0.015253
PRIMER_LEFT_25_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_25_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_25=815,20
PRIMER_RIGHT_25=1560,20
PRIMER_LEFT_25_TM=58.370
PRIMER_RIGHT_25_TM=60.015
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=4.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.5000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=746
PRIMER_PAIR_26_PENALTY=1.649445
PRIMER_LEFT_26_PENALTY=1.629679
PRIMER_RIGHT_26_PENALTY=0.019767
PRIMER_LEFT_26_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_26_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_26=815,20
PRIMER_RIGHT_26=1536,20
PRIMER_LEFT_26_TM=58.370
PRIMER_RIGHT_26_TM=60.020
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.5000
PRIMER_RIGHT_26_END_STABILITY=9.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=722
PRIMER_PAIR_27_PENALTY=1.663359
PRIMER_LEFT_27_PENALTY=1.629679
PRIMER_RIGHT_27_PENALTY=0.033680
PRIMER_LEFT_27_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_27_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_27=815,20
PRIMER_RIGHT_27=1680,20
PRIMER_LEFT_27_TM=58.370
PRIMER_RIGHT_27_TM=59.966
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=2.00
PRIMER_RIGHT_27_SELF_ANY=4.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.5000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=866
PRIMER_PAIR_28_PENALTY=1.663359
PRIMER_LEFT_28_PENALTY=1.629679
PRIMER_RIGHT_28_PENALTY=0.033680
PRIMER_LEFT_28_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_28_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_28=815,20
PRIMER_RIGHT_28=1681,20
PRIMER_LEFT_28_TM=58.370
PRIMER_RIGHT_28_TM=59.966
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=2.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=7.0000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PAIR_28_PRODUCT_SIZE=867
PRIMER_PAIR_29_PENALTY=1.689693
PRIMER_LEFT_29_PENALTY=1.674441
PRIMER_RIGHT_29_PENALTY=0.015253
PRIMER_LEFT_29_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_29_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_29=715,20
PRIMER_RIGHT_29=1560,20
PRIMER_LEFT_29_TM=58.326
PRIMER_RIGHT_29_TM=60.015
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=4.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.9000
PRIMER_RIGHT_29_END_STABILITY=8.5000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=846
PRIMER_PAIR_30_PENALTY=1.691116
PRIMER_LEFT_30_PENALTY=0.312496
PRIMER_RIGHT_30_PENALTY=1.378620
PRIMER_LEFT_30_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_30_SEQUENCE=TCTCGACTTAAAGCGGTTCCT
PRIMER_LEFT_30=814,20
PRIMER_RIGHT_30=1635,21
PRIMER_LEFT_30_TM=60.312
PRIMER_RIGHT_30_TM=60.379
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=47.619
PRIMER_LEFT_30_SELF_ANY=2.00
PRIMER_RIGHT_30_SELF_ANY=5.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.5000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=822
PRIMER_PAIR_31_PENALTY=1.694207
PRIMER_LEFT_31_PENALTY=1.674441
PRIMER_RIGHT_31_PENALTY=0.019767
PRIMER_LEFT_31_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_31_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_31=715,20
PRIMER_RIGHT_31=1536,20
PRIMER_LEFT_31_TM=58.326
PRIMER_RIGHT_31_TM=60.020
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=3.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=6.9000
PRIMER_RIGHT_31_END_STABILITY=9.7000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PAIR_31_PRODUCT_SIZE=822
PRIMER_PAIR_32_PENALTY=1.708121
PRIMER_LEFT_32_PENALTY=1.674441
PRIMER_RIGHT_32_PENALTY=0.033680
PRIMER_LEFT_32_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_32_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_32=715,20
PRIMER_RIGHT_32=1680,20
PRIMER_LEFT_32_TM=58.326
PRIMER_RIGHT_32_TM=59.966
PRIMER_LEFT_32_GC_PERCENT=50.000
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=3.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=6.9000
PRIMER_RIGHT_32_END_STABILITY=6.7000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=966
PRIMER_PAIR_33_PENALTY=1.708121
PRIMER_LEFT_33_PENALTY=1.674441
PRIMER_RIGHT_33_PENALTY=0.033680
PRIMER_LEFT_33_SEQUENCE=GGAAGGCTGTGCTTATGATG
PRIMER_RIGHT_33_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_33=715,20
PRIMER_RIGHT_33=1681,20
PRIMER_LEFT_33_TM=58.326
PRIMER_RIGHT_33_TM=59.966
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=7.0000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=967
PRIMER_PAIR_34_PENALTY=1.710108
PRIMER_LEFT_34_PENALTY=0.312496
PRIMER_RIGHT_34_PENALTY=1.397612
PRIMER_LEFT_34_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_34_SEQUENCE=CTCGACTTAAAGCGGTTCCT
PRIMER_LEFT_34=814,20
PRIMER_RIGHT_34=1634,20
PRIMER_LEFT_34_TM=60.312
PRIMER_RIGHT_34_TM=58.602
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=1.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=8.5000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=821
PRIMER_PAIR_35_PENALTY=1.738510
PRIMER_LEFT_35_PENALTY=1.230989
PRIMER_RIGHT_35_PENALTY=0.507521
PRIMER_LEFT_35_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_35_SEQUENCE=CACGCCAAAGAAAGAGAGGA
PRIMER_LEFT_35=853,20
PRIMER_RIGHT_35=1828,20
PRIMER_LEFT_35_TM=58.769
PRIMER_RIGHT_35_TM=60.508
PRIMER_LEFT_35_GC_PERCENT=40.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=6.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.0000
PRIMER_RIGHT_35_END_STABILITY=7.9000
PRIMER_PAIR_35_COMPL_ANY=6.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=976
PRIMER_PAIR_36_PENALTY=1.738510
PRIMER_LEFT_36_PENALTY=1.230989
PRIMER_RIGHT_36_PENALTY=0.507521
PRIMER_LEFT_36_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_36_SEQUENCE=TCACGCCAAAGAAAGAGAGG
PRIMER_LEFT_36=853,20
PRIMER_RIGHT_36=1829,20
PRIMER_LEFT_36_TM=58.769
PRIMER_RIGHT_36_TM=60.508
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=7.0000
PRIMER_RIGHT_36_END_STABILITY=7.9000
PRIMER_PAIR_36_COMPL_ANY=6.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PAIR_36_PRODUCT_SIZE=977
PRIMER_PAIR_37_PENALTY=1.744180
PRIMER_LEFT_37_PENALTY=1.728928
PRIMER_RIGHT_37_PENALTY=0.015253
PRIMER_LEFT_37_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_37_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_37=815,21
PRIMER_RIGHT_37=1560,20
PRIMER_LEFT_37_TM=59.271
PRIMER_RIGHT_37_TM=60.015
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=2.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=8.5000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=746
PRIMER_PAIR_38_PENALTY=1.748694
PRIMER_LEFT_38_PENALTY=1.728928
PRIMER_RIGHT_38_PENALTY=0.019767
PRIMER_LEFT_38_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_38_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_38=815,21
PRIMER_RIGHT_38=1536,20
PRIMER_LEFT_38_TM=59.271
PRIMER_RIGHT_38_TM=60.020
PRIMER_LEFT_38_GC_PERCENT=52.381
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=2.00
PRIMER_RIGHT_38_SELF_ANY=3.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.9000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=722
PRIMER_PAIR_39_PENALTY=1.762608
PRIMER_LEFT_39_PENALTY=1.728928
PRIMER_RIGHT_39_PENALTY=0.033680
PRIMER_LEFT_39_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_39_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_39=815,21
PRIMER_RIGHT_39=1680,20
PRIMER_LEFT_39_TM=59.271
PRIMER_RIGHT_39_TM=59.966
PRIMER_LEFT_39_GC_PERCENT=52.381
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.7000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=866
PRIMER_PAIR_40_PENALTY=1.762608
PRIMER_LEFT_40_PENALTY=1.728928
PRIMER_RIGHT_40_PENALTY=0.033680
PRIMER_LEFT_40_SEQUENCE=GACCACTTACCGACTTGCTGT
PRIMER_RIGHT_40_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_40=815,21
PRIMER_RIGHT_40=1681,20
PRIMER_LEFT_40_TM=59.271
PRIMER_RIGHT_40_TM=59.966
PRIMER_LEFT_40_GC_PERCENT=52.381
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=7.0000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=867
PRIMER_PAIR_41_PENALTY=1.768043
PRIMER_LEFT_41_PENALTY=0.312496
PRIMER_RIGHT_41_PENALTY=1.455547
PRIMER_LEFT_41_SEQUENCE=CGACCACTTACCGACTTGCT
PRIMER_RIGHT_41_SEQUENCE=GTTGCTCTTGATGTTCAGCGT
PRIMER_LEFT_41=814,20
PRIMER_RIGHT_41=1546,21
PRIMER_LEFT_41_TM=60.312
PRIMER_RIGHT_41_TM=60.456
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=3.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=1.00
PRIMER_LEFT_41_END_STABILITY=8.5000
PRIMER_RIGHT_41_END_STABILITY=9.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=733
PRIMER_PAIR_42_PENALTY=1.769072
PRIMER_LEFT_42_PENALTY=1.230989
PRIMER_RIGHT_42_PENALTY=0.538083
PRIMER_LEFT_42_SEQUENCE=TACCATTCGCGATTTTCTTG
PRIMER_RIGHT_42_SEQUENCE=TGGCTCTTCCTACGTTGCTC
PRIMER_LEFT_42=853,20
PRIMER_RIGHT_42=1559,20
PRIMER_LEFT_42_TM=58.769
PRIMER_RIGHT_42_TM=60.538
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=55.000
PRIMER_LEFT_42_SELF_ANY=6.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.0000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=707
PRIMER_PAIR_43_PENALTY=1.783701
PRIMER_LEFT_43_PENALTY=1.629679
PRIMER_RIGHT_43_PENALTY=0.154023
PRIMER_LEFT_43_SEQUENCE=GACCACTTACCGACTTGCTG
PRIMER_RIGHT_43_SEQUENCE=TGCCTAGCAGTTTGGCTCTT
PRIMER_LEFT_43=815,20
PRIMER_RIGHT_43=1571,20
PRIMER_LEFT_43_TM=58.370
PRIMER_RIGHT_43_TM=60.154
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=5.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=8.5000
PRIMER_RIGHT_43_END_STABILITY=6.7000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=0.00
PRIMER_PAIR_43_PRODUCT_SIZE=757
PRIMER_PAIR_44_PENALTY=1.802887
PRIMER_LEFT_44_PENALTY=1.787634
PRIMER_RIGHT_44_PENALTY=0.015253
PRIMER_LEFT_44_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_44_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_44=717,21
PRIMER_RIGHT_44=1560,20
PRIMER_LEFT_44_TM=59.212
PRIMER_RIGHT_44_TM=60.015
PRIMER_LEFT_44_GC_PERCENT=47.619
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=3.00
PRIMER_RIGHT_44_SELF_ANY=4.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=844
PRIMER_PAIR_45_PENALTY=1.802887
PRIMER_LEFT_45_PENALTY=1.787634
PRIMER_RIGHT_45_PENALTY=0.015253
PRIMER_LEFT_45_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_45_SEQUENCE=TTGGCTCTTCCTACGTTGCT
PRIMER_LEFT_45=715,21
PRIMER_RIGHT_45=1560,20
PRIMER_LEFT_45_TM=59.212
PRIMER_RIGHT_45_TM=60.015
PRIMER_LEFT_45_GC_PERCENT=47.619
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=3.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=6.3000
PRIMER_RIGHT_45_END_STABILITY=8.5000
PRIMER_PAIR_45_COMPL_ANY=5.00
PRIMER_PAIR_45_COMPL_END=0.00
PRIMER_PAIR_45_PRODUCT_SIZE=846
PRIMER_PAIR_46_PENALTY=1.807401
PRIMER_LEFT_46_PENALTY=1.787634
PRIMER_RIGHT_46_PENALTY=0.019767
PRIMER_LEFT_46_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_46_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_46=717,21
PRIMER_RIGHT_46=1536,20
PRIMER_LEFT_46_TM=59.212
PRIMER_RIGHT_46_TM=60.020
PRIMER_LEFT_46_GC_PERCENT=47.619
PRIMER_RIGHT_46_GC_PERCENT=50.000
PRIMER_LEFT_46_SELF_ANY=3.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=9.7000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=0.00
PRIMER_PAIR_46_PRODUCT_SIZE=820
PRIMER_PAIR_47_PENALTY=1.807401
PRIMER_LEFT_47_PENALTY=1.787634
PRIMER_RIGHT_47_PENALTY=0.019767
PRIMER_LEFT_47_SEQUENCE=GGAAGGCTGTGCTTATGATGT
PRIMER_RIGHT_47_SEQUENCE=ATGTTCAGCGTCTCTTGCCT
PRIMER_LEFT_47=715,21
PRIMER_RIGHT_47=1536,20
PRIMER_LEFT_47_TM=59.212
PRIMER_RIGHT_47_TM=60.020
PRIMER_LEFT_47_GC_PERCENT=47.619
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=3.00
PRIMER_RIGHT_47_SELF_ANY=3.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.3000
PRIMER_RIGHT_47_END_STABILITY=9.7000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=822
PRIMER_PAIR_48_PENALTY=1.821314
PRIMER_LEFT_48_PENALTY=1.787634
PRIMER_RIGHT_48_PENALTY=0.033680
PRIMER_LEFT_48_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_48_SEQUENCE=CCGGTGGAATCAGTTCTTGT
PRIMER_LEFT_48=717,21
PRIMER_RIGHT_48=1680,20
PRIMER_LEFT_48_TM=59.212
PRIMER_RIGHT_48_TM=59.966
PRIMER_LEFT_48_GC_PERCENT=47.619
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=3.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.7000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=0.00
PRIMER_PAIR_48_PRODUCT_SIZE=964
PRIMER_PAIR_49_PENALTY=1.821314
PRIMER_LEFT_49_PENALTY=1.787634
PRIMER_RIGHT_49_PENALTY=0.033680
PRIMER_LEFT_49_SEQUENCE=AAGGCTGTGCTTATGATGTCC
PRIMER_RIGHT_49_SEQUENCE=ACCGGTGGAATCAGTTCTTG
PRIMER_LEFT_49=717,21
PRIMER_RIGHT_49=1681,20
PRIMER_LEFT_49_TM=59.212
PRIMER_RIGHT_49_TM=59.966
PRIMER_LEFT_49_GC_PERCENT=47.619
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=3.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.9000
PRIMER_RIGHT_49_END_STABILITY=7.0000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=1.00
PRIMER_PAIR_49_PRODUCT_SIZE=965
=
SEQUENCE_ID=MAGIC.329_4_4503041_4503329_po-0_ws-0_50546_1241_TRUE
SEQUENCE_TARGET=950,388
SEQUENCE_TEMPLATE=TACCCTANAATTCCCNTTGAGCTTACTTGTTNTACCACTACATTCTCGACAATCACCGATGTTTCTAGATCGGGGACCAAGCTTAAAAAACGTTTTGTGATATGCATTCTTTTTCTGAAATGGCCACAGCCTATGAGGAGTATTCCATACGCAAAATGGGCAAACAATGAATGTACCACACATAAACGTAGCATATGAAGAGCTTCTTTTGAGGATGGACTATCTTGAGTCAATATNCTCAACGATTCTACTTACCNTGCTGTGGATGTCAGATTCACACANTCAAGTATTTNTTTATTTAGTGAGAAGGATTATTCTTNAGGACAAATGTANTNGAAAATAGATTGATGATTATAGAGAAAACTATGAGNTTTNTAANAAAATGTGTATANNANNTTTGCACAAAAATGCNNGTNNNNCTCTACATGGTCTTTGACCCACAATTTATACTCAATGAATACATTTAATTACCATTTATACACAATTATTCTATATTAAATGTGTCTGTTTCAGTCTTCAAATTAACTATTCGAACGTTCGAATTTGAATNGNTTCCAAAATTTCTGGGCTTCGTCCTCTGGCCCANNNGACTCTCCAATCCGGGCAGTTCTGACATCGATGTCCAGTCCACCTNGCNNNNCNNTCACCTCACNTNTACTTTTACTCAGAAAAGCCCATGGGCCTTTCCTCTTTTAGATCNNTNCNNCTTACGNGGCGATCTTTGACCGNTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNATNCCTNAATGNNCAAAGATNNNNCNNAATGACTCTCTGANTTCCCGCCAANNNNNNNNNNNNNNNNNNNNNNNGGAATCACCCATAATTACTCTCGTGACATCTGTCANAGTTATGACCNCATANCAATTATNTACTATTNAANCGGNAAAAACTNTTCCACNTCCTCCANGACTNCCAAGGCNGTTNCGTCATNATCNCTAGCTTGCGTAAGGTAANTTCNCTTTNNNNNNNNNNNNNNNNNNNNCANNNTTNTTTTANAACAAAGCNCCTNTCTTCTTCCTNNNNNNNNNNNNNNNNNNNNGAACCCTCAGACNANNCATTTTTANGATNAATTCNNAAANTGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAANGANNTTTGCACNCGNNNGAATGANATNGCCGNNCCNCANTANCANTCCACNTGNACNCTNCAANCCCTNAATNGATTGNGAGAGCTNTGTCGNNNNNNNNNNNNNANTATGAGGGANTCNCANATGCCNNANCCNANAGANTCGCCGNAAGACCANNNTNCNGGGTTCTTCTNCNTGTNCGANATNTANTTCAAGGGTTGCGGTNTGANNTTTNCNCTTCCNGNAGCCCTNGTTCNGTACTTANCNGCCCTNNAAATTGCCNTGCCCNNNNTNACCCCTNACNTNCTNCGNNCTATNTTAGNGATTATTACNATCGCNNCNNANGCCGGATNTGTNATCNGNGTTCCNNAACTGAATGAACTTCTCAGCNTGAGGAGTTCTTCNAAGAAAGNNGNATACTTCTNGGCTTATNCGAANGCCNACANGAANCTCATCTNNNATCTTNCNAACAANGACGANAACTNGCANCANCCNTGGTTNNTAANNNNNNNNNNNNNNNNNNNNNTNGNAAATCTTTCCGACNTGCTCCCCNNGAAGNCNNGTAGNNCTTAGGTCATGCNTATGATTTTTATANANATNTTGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGAAACTCTTTGGAATTCCTTCACCNTNGACNGNTTCATANAGGCNAACCACAAGCTCANGANGAGTCNCCCTGNACAACTTCTTCAACTTCNGTTNCCTCCTCCTTTACCTCANNGANCNTCNNCTCGAGNCATCGCCNCCNGANGNAAGANGNTGTCCAAATCNATCGTGGAGGCTTGTGANGNGAACAANTNNTTCNTTACGTNGGNTNTNGANAAGAAGGAGTACAGNAGAACCCNGNTANTNNANGNTGGNAGTNCCNAAGGNGNNAGATCNGNNNNTGCCTTCAGACCCANNCCTCANNNTGAAGGTCATGGTNGNNNNTCACCTCNNNGAGACNNATCACCTCNTCGTGANTCACCTNNGCCTNCNTNTNGNAATAGGTTGAGCAGNGAANCNATCNCG
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_1_12750462_12750830_ler-0_wil-2_83956_1837_TRUE
SEQUENCE_TARGET=950,468
SEQUENCE_TEMPLATE=GCAACAAGAGAATCAAGNATTCGGGTTCCTCGGTANCGTGCCCGCATTNTCGAATTCAAAAGNAAGAGAGTGGGAAACCCATGAACTCCATACTTGGAAAGGGTNCTGCAGCANAACAANTAACCAAAAGATCATCACCANAACANANNNNNNNNNNNNNNNNNNNNNACCAGTTTAAANATNNTCACCTAGGCTTAANGGATGATTCCTTAATGGCAAAATGAGGAANGGAGGAATAGAGAGAAGAGATAACATCNAAACTNGGTCTAAATGACCTTGANAATGGACACCATGATGCATAGAAAAGCAAAGCCACATAATCACACTTGTTCNTATGAATCATATCCAATGCTATTTGCAACCAACGCTCATCACCCTNCANCAACATTTGANNACATTCATAAGAAACNAAAGANAGATTCTTNNTCAAGCAGAAACCCTAGAAATTGAAATCNNNNNNNNNNNNTNAATTACCTCGGTGACAGCAACAAAACGAGGACGCTCGTCTGATTCAACACCAGAAACAGAACAAGTCTGATCTCGNNGCCCAAAGATNGAATCTTTANCAGANTTCGTAGCACAAAACGGAACCCTAACGGCGTNGACGTCGGCNACGNTTAAAAACATGATGACCAATAGCAACAACNAGATCTCCTTNTCCATNAAANAANACCCAGAANCTCTCTCTCTCTCTATCTCNNNNNNTCTCCCTACGAAATTCACAGAACCCAGAATAGAAATTNGTTGTTGTTGCCGACAAATTGGAAACAGGTGAGAGGTTGATGTTTCTGGGAATTGAATCAATGNTGTTTCTTTANTGANGAGATNTTTATGCAGCGGTAANGGNNNNNNNNATTTGAGGGAGATATNGANGAAGAAGAANAGGTTGTGGTTGCTGCTGGGNACTGGGTTNTTGCTCGTAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGTCATGACAAAATTAGNNANAAAANAAANTCTAAACCAGNATTTTTANTAAACCGNNNNTTTTNTTTAGTANANTCAGAATTTTTGCTTCAATACGATTTTGATTTCGNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATATTTCAACACGTNGNAAGAAAGANTNTTATTNANNANNAGNNTTTCNNNAATNAAAANTTATATTGNTNNNNNNATACAGTTAATTGTTATTTNANATNGTNTNNNNNTNTNTNTNTTTATTAATATGAGCATTGAAACATATTAAGTTNTCGACACNGTNTATATTTGACCATAATNGNNTTAATANANNNNCATTATAAACACNATTCGGTTNGTTNNTNATGCNNNNNNNNNNAANATCTCTCCCTTTTNCNNNGTTCAAGTNNNCNNNANNACNNTTTNTCNGCAAAGTCNTNATATNCAGNNNTTTTTCCCACNATATCATGNTTTGNTTNNATNTCCNANNNNNNNNNNNANTCTTGTATNCAATTTGTTGATGTGGNGATTGTCNNAGTCCTGAAGTNTACTTANAGTTGGAATCCTNTCAAGGTAGANGNNNANNATCNNGNGNATATNTNTTNCNNTNNACNTTNGNNNTNNTGTNNNNNCATTTTTACCATGTANTAANNNNAAATGTTTATTTNAGAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.338_5_14079240_14079355_zu-0_tsu-0_11089_15006_TRUE
SEQUENCE_TARGET=950,215
SEQUENCE_TEMPLATE=AATGCAATTGTAAAGAAAAAANATCTACTNTNNNNNNNNAAGTTGATAAATGAGATATGTTTCTCTTTATAATNTAAGAGCAAAATCATCAAAACATATAATTTTATTTTTATTTATTTATTGGTACTAATTTGTTTTGATATATAATATTTGTTTAGTGGTTTTTTCTTAAATTAAGATTTGATGGGCGTCAAATTTTATTTTTGAATGGGGTCAATATATCAATNANTAAATAAANTAAGCTAAATTTTAGAAATTCATGGGTGTCAATTGACCCCATGGTAAAGTGCTANCCCCGCCACTACTGATAATGAAATAGAAAGATTTGGTGTCTGTTATTGTTGTTGTTTTTAAACTCATAACAAAACCTTCATGCTTGTAGCTTCTCTAATTTGGGTACAGATNCTTGATTCAACGCTCAAAGTGGTTTTAAGGCCTAAGCAGGAAATGATATTCGATGAACCGGAGCTGCCATAAATCTTTGAGAATTTTGGATACATTGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_18551947_18552277_ler-0_po-0_12476_45881_TRUE
SEQUENCE_TARGET=950,430
SEQUENCE_TEMPLATE=TTTTTNAAGTTTTGTCANTCTCGATAATTTTATAGAAAAATGTGGAGGGAGGGAATCATTTTGTATCANACCNAGTGACATGTNANNTAAATAAANNNNNNNNNNNNNNNNNTAGAAAAANCTNNAATTTTCTTGTNNNNNNNNNNNNNNNNNNNNCCTTGTTCTTGTNNTGTAANTCAAAGCTGANNNNNNNNNNNCAAAATACTTAGAANTTGTCAACTGTCAAAAGTTCATTAGATCNTTTTGACCCCAACAAAGTNTNACAAATCCTNNCTGTACNTNTGTACCTCAAACACCACGTNTCACCATCTCNCACTTTAGATTNCAGNNACTANCAATTGCTAAAAGTNTTCAATATGANNNNNGAGACTTCAGATTCTAGNNNNNNNNNNNNNTCTTCTTCTNCTACTNNNNNNTACTAAGCTTTGCTTTTTGTNNNAANNTTCATGGAANTTACTGTCCCTCCTCTNGAATCAGATTCGTGGGCTCACTTAGAANATACCTAGGAAGCTCATATCTAGCACCTGCATTGAGTAAGTATAAGCCAGTNNGTAACTTANANNNNNNNNNANAGAAACGAATTAGNTCANAAGTAGATTATGNTTGATTGTCANCNTCNTCGAATCTTTTGAACTAAGCNGAAGTTCCAANANAAACNTGATAACATTGCTAGATGGANCCATCGACTTTGCTACATNACATTNAATTCTNTAGTTGTTAANGAATTTTGGGCTTAACTTNAAAAGTACAAAGAGGGTCTTTTATTATTNGNACATGGNCCANTTAAACATTGAGCCGGGCCTGGTTATAAGCAGAACATTTGNGNATATTTNAAAATAATGGACTNNNNNNNNAANNATTTNCTTGATCCGTATCATCAATTTATAAATNGTGTAANCTGNCACGATNAGCTGGTTGTGTCGGTAAGGTCAATGACTGTNGGNANTTTACAAACTTGTGCCGTTTGATATTTCAATCTTTNGTCTTTTACCAANNNAAANACTCAAAGCAGATNNNNNNNNNNNNCTAATNGACCAACAAAGCGAGAGAGTGAAGCAATTCTGCAATCANCCGTTAAGATACGCAAGAATTAAGCAGGAGNGAGTAATTACAGGTNGGTCAAATAACTGCGATGGATGCGGTGATGATTCAGGCTACGGATATTACTGTGAACNCTNCAATATTCAAGCTCATGTGGCTTGCATAGGTTGGCCAGACATCATCAACCACCCCTGTCACTCCAGACANNCTCTCAAGAAAGTCTCGCCAGAGACAATTGACTATACTGATGGTAAATGCCAGTTTTGTAGAAGCCCACTTGTTGATNTCANGTATCATTGTTCCATTTGTAATTTCNGTGTNGATTTGNGATGCTGGTTNNANCCCGCACCGCTTANTATATATGAGCAAAAGAGCCACANCCATACATTAACCCTNATGGCGAGAAANGACGCCTTCACTTGTAACGCATGTGGGTTGGTTGGTGACCGNAATCCTTACGTTTGTCTTGAATGTGATTTCATGCTGCACAAANGTTGTATCAATTTNCCNCGAGTCATAAACATNAATCGTCATGACCANAAGATTTCTCGCACTTATCATCTCGGTCACGGNGATTGGGAATGTGGAGTTTGTCGCCAAANGATGGATTGGACCCTTGGAGCTTTCTNTTGNTCGCGTTGTCCTAATTATGCTGTTCATTCNAAGTGTGCNACAAGGGAAGATGTGTGGGATGGANAAGAACTCGAAGANAAGCCTGAAGATGAGGNAGAAGAGGATCCATACNAGGTNATAAATGAGAAAGAAATCATTCANTTTNNNNNNNNNNNNNNNNNNNNNNNACTTGGTGATGATGATAATGNTGCNNATTATGAAAAGATGNGGTGNNANNNNTGCATCANGCCCATCNATTCTGATCCATTCTTNAAATGTGTGGAATGCNAGTTCTTCCTTCATAAAGTATGTGCCNNTCTTCCCCNGAAGAAACGAANTATCCTGCANANNNANNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACAANTTGTCNTNAATNTTTTGATGGCTTCNNGTACAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTATTNGATATNCGGTGNAGTTCCATNTCNGAGCCTTTTCAGCATNANCTGCATCCCCATCCNTTATTTCGTACCTCNAAAGAGNNNAAGANNTGTGGAGCATGTGGTGAAGATTCNGAGTATGTNTTAAGNTGTATTGTTTGTGANTTCNCTTTAGGCATGGANTGTGCCACTTTACCAAGAAAAGTNAAACACAGATG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=0.062489
PRIMER_LEFT_0_PENALTY=0.035367
PRIMER_RIGHT_0_PENALTY=0.027122
PRIMER_LEFT_0_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_0_SEQUENCE=CAACCCACATGCGTTACAAG
PRIMER_LEFT_0=910,20
PRIMER_RIGHT_0=1478,20
PRIMER_LEFT_0_TM=60.035
PRIMER_RIGHT_0_TM=60.027
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=2.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.9000
PRIMER_RIGHT_0_END_STABILITY=6.7000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=569
PRIMER_PAIR_1_PENALTY=0.197994
PRIMER_LEFT_1_PENALTY=0.170871
PRIMER_RIGHT_1_PENALTY=0.027122
PRIMER_LEFT_1_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_1_SEQUENCE=CAACCCACATGCGTTACAAG
PRIMER_LEFT_1=909,20
PRIMER_RIGHT_1=1478,20
PRIMER_LEFT_1_TM=60.171
PRIMER_RIGHT_1_TM=60.027
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PAIR_1_PRODUCT_SIZE=570
PRIMER_PAIR_2_PENALTY=0.256212
PRIMER_LEFT_2_PENALTY=0.035367
PRIMER_RIGHT_2_PENALTY=0.220845
PRIMER_LEFT_2_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_2_SEQUENCE=ATCCCACACATCTTCCCTTG
PRIMER_LEFT_2=910,20
PRIMER_RIGHT_2=1733,20
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=824
PRIMER_PAIR_3_PENALTY=0.256212
PRIMER_LEFT_3_PENALTY=0.035367
PRIMER_RIGHT_3_PENALTY=0.220845
PRIMER_LEFT_3_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_3_SEQUENCE=CATCCCACACATCTTCCCTT
PRIMER_LEFT_3=910,20
PRIMER_RIGHT_3=1734,20
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.779
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=825
PRIMER_PAIR_4_PENALTY=0.391716
PRIMER_LEFT_4_PENALTY=0.170871
PRIMER_RIGHT_4_PENALTY=0.220845
PRIMER_LEFT_4_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_4_SEQUENCE=ATCCCACACATCTTCCCTTG
PRIMER_LEFT_4=909,20
PRIMER_RIGHT_4=1733,20
PRIMER_LEFT_4_TM=60.171
PRIMER_RIGHT_4_TM=59.779
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=825
PRIMER_PAIR_5_PENALTY=0.398356
PRIMER_LEFT_5_PENALTY=0.035367
PRIMER_RIGHT_5_PENALTY=0.362989
PRIMER_LEFT_5_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_5_SEQUENCE=TCCCACACATCTTCCCTTGT
PRIMER_LEFT_5=910,20
PRIMER_RIGHT_5=1732,20
PRIMER_LEFT_5_TM=60.035
PRIMER_RIGHT_5_TM=60.363
PRIMER_LEFT_5_GC_PERCENT=55.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=823
PRIMER_PAIR_6_PENALTY=0.478265
PRIMER_LEFT_6_PENALTY=0.035367
PRIMER_RIGHT_6_PENALTY=0.442898
PRIMER_LEFT_6_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_6_SEQUENCE=AAAGCTCCAAGGGTCCAATC
PRIMER_LEFT_6=910,20
PRIMER_RIGHT_6=1666,20
PRIMER_LEFT_6_TM=60.035
PRIMER_RIGHT_6_TM=60.443
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PAIR_6_PRODUCT_SIZE=757
PRIMER_PAIR_7_PENALTY=0.533861
PRIMER_LEFT_7_PENALTY=0.170871
PRIMER_RIGHT_7_PENALTY=0.362989
PRIMER_LEFT_7_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_7_SEQUENCE=TCCCACACATCTTCCCTTGT
PRIMER_LEFT_7=909,20
PRIMER_RIGHT_7=1732,20
PRIMER_LEFT_7_TM=60.171
PRIMER_RIGHT_7_TM=60.363
PRIMER_LEFT_7_GC_PERCENT=55.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=7.5000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=824
PRIMER_PAIR_8_PENALTY=0.613769
PRIMER_LEFT_8_PENALTY=0.170871
PRIMER_RIGHT_8_PENALTY=0.442898
PRIMER_LEFT_8_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_8_SEQUENCE=AAAGCTCCAAGGGTCCAATC
PRIMER_LEFT_8=909,20
PRIMER_RIGHT_8=1666,20
PRIMER_LEFT_8_TM=60.171
PRIMER_RIGHT_8_TM=60.443
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=7.5000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=758
PRIMER_PAIR_9_PENALTY=1.059579
PRIMER_LEFT_9_PENALTY=0.035367
PRIMER_RIGHT_9_PENALTY=1.024212
PRIMER_LEFT_9_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_9_SEQUENCE=CCGAGATGATAAGTGCGAGA
PRIMER_LEFT_9=910,20
PRIMER_RIGHT_9=1606,20
PRIMER_LEFT_9_TM=60.035
PRIMER_RIGHT_9_TM=58.976
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=697
PRIMER_PAIR_10_PENALTY=1.158141
PRIMER_LEFT_10_PENALTY=0.035367
PRIMER_RIGHT_10_PENALTY=1.122774
PRIMER_LEFT_10_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_10_SEQUENCE=AGAAAGCTCCAAGGGTCCAA
PRIMER_LEFT_10=910,20
PRIMER_RIGHT_10=1668,20
PRIMER_LEFT_10_TM=60.035
PRIMER_RIGHT_10_TM=61.123
PRIMER_LEFT_10_GC_PERCENT=55.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=759
PRIMER_PAIR_11_PENALTY=1.184365
PRIMER_LEFT_11_PENALTY=0.035367
PRIMER_RIGHT_11_PENALTY=1.148998
PRIMER_LEFT_11_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_11_SEQUENCE=ACCGAGATGATAAGTGCGAGA
PRIMER_LEFT_11=910,20
PRIMER_RIGHT_11=1607,21
PRIMER_LEFT_11_TM=60.035
PRIMER_RIGHT_11_TM=59.851
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=47.619
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PAIR_11_PRODUCT_SIZE=698
PRIMER_PAIR_12_PENALTY=1.184365
PRIMER_LEFT_12_PENALTY=0.035367
PRIMER_RIGHT_12_PENALTY=1.148998
PRIMER_LEFT_12_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_12_SEQUENCE=GACCGAGATGATAAGTGCGAG
PRIMER_LEFT_12=910,20
PRIMER_RIGHT_12=1608,21
PRIMER_LEFT_12_TM=60.035
PRIMER_RIGHT_12_TM=59.851
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=9.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=699
PRIMER_PAIR_13_PENALTY=1.195083
PRIMER_LEFT_13_PENALTY=0.170871
PRIMER_RIGHT_13_PENALTY=1.024212
PRIMER_LEFT_13_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_13_SEQUENCE=CCGAGATGATAAGTGCGAGA
PRIMER_LEFT_13=909,20
PRIMER_RIGHT_13=1606,20
PRIMER_LEFT_13_TM=60.171
PRIMER_RIGHT_13_TM=58.976
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=7.5000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=698
PRIMER_PAIR_14_PENALTY=1.209243
PRIMER_LEFT_14_PENALTY=0.035367
PRIMER_RIGHT_14_PENALTY=1.173876
PRIMER_LEFT_14_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_14_SEQUENCE=GACCGAGATGATAAGTGCGA
PRIMER_LEFT_14=910,20
PRIMER_RIGHT_14=1608,20
PRIMER_LEFT_14_TM=60.035
PRIMER_RIGHT_14_TM=58.826
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=10.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=699
PRIMER_PAIR_15_PENALTY=1.293646
PRIMER_LEFT_15_PENALTY=0.170871
PRIMER_RIGHT_15_PENALTY=1.122774
PRIMER_LEFT_15_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_15_SEQUENCE=AGAAAGCTCCAAGGGTCCAA
PRIMER_LEFT_15=909,20
PRIMER_RIGHT_15=1668,20
PRIMER_LEFT_15_TM=60.171
PRIMER_RIGHT_15_TM=61.123
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=7.5000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=760
PRIMER_PAIR_16_PENALTY=1.319870
PRIMER_LEFT_16_PENALTY=0.170871
PRIMER_RIGHT_16_PENALTY=1.148998
PRIMER_LEFT_16_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_16_SEQUENCE=ACCGAGATGATAAGTGCGAGA
PRIMER_LEFT_16=909,20
PRIMER_RIGHT_16=1607,21
PRIMER_LEFT_16_TM=60.171
PRIMER_RIGHT_16_TM=59.851
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=47.619
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=7.5000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=699
PRIMER_PAIR_17_PENALTY=1.319870
PRIMER_LEFT_17_PENALTY=0.170871
PRIMER_RIGHT_17_PENALTY=1.148998
PRIMER_LEFT_17_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_17_SEQUENCE=GACCGAGATGATAAGTGCGAG
PRIMER_LEFT_17=909,20
PRIMER_RIGHT_17=1608,21
PRIMER_LEFT_17_TM=60.171
PRIMER_RIGHT_17_TM=59.851
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.5000
PRIMER_RIGHT_17_END_STABILITY=9.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PAIR_17_PRODUCT_SIZE=700
PRIMER_PAIR_18_PENALTY=1.344748
PRIMER_LEFT_18_PENALTY=0.170871
PRIMER_RIGHT_18_PENALTY=1.173876
PRIMER_LEFT_18_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_18_SEQUENCE=GACCGAGATGATAAGTGCGA
PRIMER_LEFT_18=909,20
PRIMER_RIGHT_18=1608,20
PRIMER_LEFT_18_TM=60.171
PRIMER_RIGHT_18_TM=58.826
PRIMER_LEFT_18_GC_PERCENT=55.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=7.5000
PRIMER_RIGHT_18_END_STABILITY=10.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=700
PRIMER_PAIR_19_PENALTY=1.394099
PRIMER_LEFT_19_PENALTY=0.035367
PRIMER_RIGHT_19_PENALTY=1.358732
PRIMER_LEFT_19_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_19_SEQUENCE=CCGAGATGATAAGTGCGAGAA
PRIMER_LEFT_19=910,20
PRIMER_RIGHT_19=1606,21
PRIMER_LEFT_19_TM=60.035
PRIMER_RIGHT_19_TM=60.359
PRIMER_LEFT_19_GC_PERCENT=55.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PAIR_19_PRODUCT_SIZE=697
PRIMER_PAIR_20_PENALTY=1.509872
PRIMER_LEFT_20_PENALTY=0.035367
PRIMER_RIGHT_20_PENALTY=1.474506
PRIMER_LEFT_20_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_20_SEQUENCE=TTGGCGACAAACTCCACATT
PRIMER_LEFT_20=910,20
PRIMER_RIGHT_20=1640,20
PRIMER_LEFT_20_TM=60.035
PRIMER_RIGHT_20_TM=61.475
PRIMER_LEFT_20_GC_PERCENT=55.000
PRIMER_RIGHT_20_GC_PERCENT=45.000
PRIMER_LEFT_20_SELF_ANY=2.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=731
PRIMER_PAIR_21_PENALTY=1.529604
PRIMER_LEFT_21_PENALTY=0.170871
PRIMER_RIGHT_21_PENALTY=1.358732
PRIMER_LEFT_21_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_21_SEQUENCE=CCGAGATGATAAGTGCGAGAA
PRIMER_LEFT_21=909,20
PRIMER_RIGHT_21=1606,21
PRIMER_LEFT_21_TM=60.171
PRIMER_RIGHT_21_TM=60.359
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=47.619
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=7.5000
PRIMER_RIGHT_21_END_STABILITY=6.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=698
PRIMER_PAIR_22_PENALTY=1.557759
PRIMER_LEFT_22_PENALTY=1.530636
PRIMER_RIGHT_22_PENALTY=0.027122
PRIMER_LEFT_22_SEQUENCE=GGTTGTGTCGGTAAGGTCAA
PRIMER_RIGHT_22_SEQUENCE=CAACCCACATGCGTTACAAG
PRIMER_LEFT_22=913,20
PRIMER_RIGHT_22=1478,20
PRIMER_LEFT_22_TM=58.469
PRIMER_RIGHT_22_TM=60.027
PRIMER_LEFT_22_GC_PERCENT=50.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.7000
PRIMER_RIGHT_22_END_STABILITY=6.7000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PAIR_22_PRODUCT_SIZE=566
PRIMER_PAIR_23_PENALTY=1.558158
PRIMER_LEFT_23_PENALTY=0.035367
PRIMER_RIGHT_23_PENALTY=1.522792
PRIMER_LEFT_23_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_23_SEQUENCE=CCGTGACCGAGATGATAAGTG
PRIMER_LEFT_23=910,20
PRIMER_RIGHT_23=1612,21
PRIMER_LEFT_23_TM=60.035
PRIMER_RIGHT_23_TM=60.523
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=52.381
PRIMER_LEFT_23_SELF_ANY=2.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=7.9000
PRIMER_RIGHT_23_END_STABILITY=6.7000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PAIR_23_PRODUCT_SIZE=703
PRIMER_PAIR_24_PENALTY=1.645377
PRIMER_LEFT_24_PENALTY=0.170871
PRIMER_RIGHT_24_PENALTY=1.474506
PRIMER_LEFT_24_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_24_SEQUENCE=TTGGCGACAAACTCCACATT
PRIMER_LEFT_24=909,20
PRIMER_RIGHT_24=1640,20
PRIMER_LEFT_24_TM=60.171
PRIMER_RIGHT_24_TM=61.475
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=45.000
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.5000
PRIMER_RIGHT_24_END_STABILITY=6.6000
PRIMER_PAIR_24_COMPL_ANY=6.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=732
PRIMER_PAIR_25_PENALTY=1.657969
PRIMER_LEFT_25_PENALTY=0.035367
PRIMER_RIGHT_25_PENALTY=1.622602
PRIMER_LEFT_25_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_25_SEQUENCE=ATCCCACACATCTTCCCTTGT
PRIMER_LEFT_25=910,20
PRIMER_RIGHT_25=1733,21
PRIMER_LEFT_25_TM=60.035
PRIMER_RIGHT_25_TM=60.623
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=47.619
PRIMER_LEFT_25_SELF_ANY=2.00
PRIMER_RIGHT_25_SELF_ANY=3.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=7.9000
PRIMER_RIGHT_25_END_STABILITY=6.7000
PRIMER_PAIR_25_COMPL_ANY=6.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PAIR_25_PRODUCT_SIZE=824
PRIMER_PAIR_26_PENALTY=1.663532
PRIMER_LEFT_26_PENALTY=0.035367
PRIMER_RIGHT_26_PENALTY=1.628165
PRIMER_LEFT_26_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_26_SEQUENCE=CTCCAAGGGTCCAATCCATC
PRIMER_LEFT_26=910,20
PRIMER_RIGHT_26=1662,20
PRIMER_LEFT_26_TM=60.035
PRIMER_RIGHT_26_TM=61.628
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=2.00
PRIMER_RIGHT_26_SELF_ANY=3.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=7.9000
PRIMER_RIGHT_26_END_STABILITY=8.1000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=753
PRIMER_PAIR_27_PENALTY=1.693663
PRIMER_LEFT_27_PENALTY=0.170871
PRIMER_RIGHT_27_PENALTY=1.522792
PRIMER_LEFT_27_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_27_SEQUENCE=CCGTGACCGAGATGATAAGTG
PRIMER_LEFT_27=909,20
PRIMER_RIGHT_27=1612,21
PRIMER_LEFT_27_TM=60.171
PRIMER_RIGHT_27_TM=60.523
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=52.381
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=3.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=7.5000
PRIMER_RIGHT_27_END_STABILITY=6.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=704
PRIMER_PAIR_28_PENALTY=1.751481
PRIMER_LEFT_28_PENALTY=1.530636
PRIMER_RIGHT_28_PENALTY=0.220845
PRIMER_LEFT_28_SEQUENCE=GGTTGTGTCGGTAAGGTCAA
PRIMER_RIGHT_28_SEQUENCE=ATCCCACACATCTTCCCTTG
PRIMER_LEFT_28=913,20
PRIMER_RIGHT_28=1733,20
PRIMER_LEFT_28_TM=58.469
PRIMER_RIGHT_28_TM=59.779
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=50.000
PRIMER_LEFT_28_SELF_ANY=3.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=8.5000
PRIMER_PAIR_28_COMPL_ANY=6.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=821
PRIMER_PAIR_29_PENALTY=1.751481
PRIMER_LEFT_29_PENALTY=1.530636
PRIMER_RIGHT_29_PENALTY=0.220845
PRIMER_LEFT_29_SEQUENCE=GGTTGTGTCGGTAAGGTCAA
PRIMER_RIGHT_29_SEQUENCE=CATCCCACACATCTTCCCTT
PRIMER_LEFT_29=913,20
PRIMER_RIGHT_29=1734,20
PRIMER_LEFT_29_TM=58.469
PRIMER_RIGHT_29_TM=59.779
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=50.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=6.7000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=3.00
PRIMER_PAIR_29_PRODUCT_SIZE=822
PRIMER_PAIR_30_PENALTY=1.783941
PRIMER_LEFT_30_PENALTY=0.170871
PRIMER_RIGHT_30_PENALTY=1.613069
PRIMER_LEFT_30_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_30_SEQUENCE=GACAAACTCCACATTCCCAA
PRIMER_LEFT_30=909,20
PRIMER_RIGHT_30=1635,20
PRIMER_LEFT_30_TM=60.171
PRIMER_RIGHT_30_TM=58.387
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=45.000
PRIMER_LEFT_30_SELF_ANY=4.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=1.00
PRIMER_LEFT_30_END_STABILITY=7.5000
PRIMER_RIGHT_30_END_STABILITY=10.0000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=727
PRIMER_PAIR_31_PENALTY=1.793474
PRIMER_LEFT_31_PENALTY=0.170871
PRIMER_RIGHT_31_PENALTY=1.622602
PRIMER_LEFT_31_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_31_SEQUENCE=ATCCCACACATCTTCCCTTGT
PRIMER_LEFT_31=909,20
PRIMER_RIGHT_31=1733,21
PRIMER_LEFT_31_TM=60.171
PRIMER_RIGHT_31_TM=60.623
PRIMER_LEFT_31_GC_PERCENT=55.000
PRIMER_RIGHT_31_GC_PERCENT=47.619
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=3.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=7.5000
PRIMER_RIGHT_31_END_STABILITY=6.7000
PRIMER_PAIR_31_COMPL_ANY=6.00
PRIMER_PAIR_31_COMPL_END=2.00
PRIMER_PAIR_31_PRODUCT_SIZE=825
PRIMER_PAIR_32_PENALTY=1.799036
PRIMER_LEFT_32_PENALTY=0.170871
PRIMER_RIGHT_32_PENALTY=1.628165
PRIMER_LEFT_32_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_32_SEQUENCE=CTCCAAGGGTCCAATCCATC
PRIMER_LEFT_32=909,20
PRIMER_RIGHT_32=1662,20
PRIMER_LEFT_32_TM=60.171
PRIMER_RIGHT_32_TM=61.628
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=3.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=7.5000
PRIMER_RIGHT_32_END_STABILITY=8.1000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PAIR_32_PRODUCT_SIZE=754
PRIMER_PAIR_33_PENALTY=1.893626
PRIMER_LEFT_33_PENALTY=1.530636
PRIMER_RIGHT_33_PENALTY=0.362989
PRIMER_LEFT_33_SEQUENCE=GGTTGTGTCGGTAAGGTCAA
PRIMER_RIGHT_33_SEQUENCE=TCCCACACATCTTCCCTTGT
PRIMER_LEFT_33=913,20
PRIMER_RIGHT_33=1732,20
PRIMER_LEFT_33_TM=58.469
PRIMER_RIGHT_33_TM=60.363
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=3.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=6.7000
PRIMER_PAIR_33_COMPL_ANY=6.00
PRIMER_PAIR_33_COMPL_END=2.00
PRIMER_PAIR_33_PRODUCT_SIZE=820
PRIMER_PAIR_34_PENALTY=1.933582
PRIMER_LEFT_34_PENALTY=0.035367
PRIMER_RIGHT_34_PENALTY=1.898215
PRIMER_LEFT_34_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_34_SEQUENCE=CGACAAACTCCACATTCCCA
PRIMER_LEFT_34=910,20
PRIMER_RIGHT_34=1636,20
PRIMER_LEFT_34_TM=60.035
PRIMER_RIGHT_34_TM=61.898
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=2.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=7.9000
PRIMER_RIGHT_34_END_STABILITY=9.7000
PRIMER_PAIR_34_COMPL_ANY=6.00
PRIMER_PAIR_34_COMPL_END=3.00
PRIMER_PAIR_34_PRODUCT_SIZE=727
PRIMER_PAIR_35_PENALTY=1.968415
PRIMER_LEFT_35_PENALTY=1.941293
PRIMER_RIGHT_35_PENALTY=0.027122
PRIMER_LEFT_35_SEQUENCE=CTGGTTGTGTCGGTAAGGTC
PRIMER_RIGHT_35_SEQUENCE=CAACCCACATGCGTTACAAG
PRIMER_LEFT_35=911,20
PRIMER_RIGHT_35=1478,20
PRIMER_LEFT_35_TM=58.059
PRIMER_RIGHT_35_TM=60.027
PRIMER_LEFT_35_GC_PERCENT=55.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=2.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=1.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=7.6000
PRIMER_RIGHT_35_END_STABILITY=6.7000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PAIR_35_PRODUCT_SIZE=568
PRIMER_PAIR_36_PENALTY=1.973534
PRIMER_LEFT_36_PENALTY=1.530636
PRIMER_RIGHT_36_PENALTY=0.442898
PRIMER_LEFT_36_SEQUENCE=GGTTGTGTCGGTAAGGTCAA
PRIMER_RIGHT_36_SEQUENCE=AAAGCTCCAAGGGTCCAATC
PRIMER_LEFT_36=913,20
PRIMER_RIGHT_36=1666,20
PRIMER_LEFT_36_TM=58.469
PRIMER_RIGHT_36_TM=60.443
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=3.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=6.9000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=754
PRIMER_PAIR_37_PENALTY=2.002716
PRIMER_LEFT_37_PENALTY=1.975594
PRIMER_RIGHT_37_PENALTY=0.027122
PRIMER_LEFT_37_SEQUENCE=AGCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_37_SEQUENCE=CAACCCACATGCGTTACAAG
PRIMER_LEFT_37=909,21
PRIMER_RIGHT_37=1478,20
PRIMER_LEFT_37_TM=60.976
PRIMER_RIGHT_37_TM=60.027
PRIMER_LEFT_37_GC_PERCENT=52.381
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=7.9000
PRIMER_RIGHT_37_END_STABILITY=6.7000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=570
PRIMER_PAIR_38_PENALTY=2.069087
PRIMER_LEFT_38_PENALTY=0.170871
PRIMER_RIGHT_38_PENALTY=1.898215
PRIMER_LEFT_38_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_38_SEQUENCE=CGACAAACTCCACATTCCCA
PRIMER_LEFT_38=909,20
PRIMER_RIGHT_38=1636,20
PRIMER_LEFT_38_TM=60.171
PRIMER_RIGHT_38_TM=61.898
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=50.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=7.5000
PRIMER_RIGHT_38_END_STABILITY=9.7000
PRIMER_PAIR_38_COMPL_ANY=6.00
PRIMER_PAIR_38_COMPL_END=2.00
PRIMER_PAIR_38_PRODUCT_SIZE=728
PRIMER_PAIR_39_PENALTY=2.088473
PRIMER_LEFT_39_PENALTY=0.035367
PRIMER_RIGHT_39_PENALTY=2.053107
PRIMER_LEFT_39_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_39_SEQUENCE=TGGCGACAAACTCCACATTC
PRIMER_LEFT_39=910,20
PRIMER_RIGHT_39=1639,20
PRIMER_LEFT_39_TM=60.035
PRIMER_RIGHT_39_TM=62.053
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=2.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=7.9000
PRIMER_RIGHT_39_END_STABILITY=6.9000
PRIMER_PAIR_39_COMPL_ANY=6.00
PRIMER_PAIR_39_COMPL_END=2.00
PRIMER_PAIR_39_PRODUCT_SIZE=730
PRIMER_PAIR_40_PENALTY=2.113662
PRIMER_LEFT_40_PENALTY=0.035367
PRIMER_RIGHT_40_PENALTY=2.078295
PRIMER_LEFT_40_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_40_SEQUENCE=ACCGAGATGATAAGTGCGAG
PRIMER_LEFT_40=910,20
PRIMER_RIGHT_40=1607,20
PRIMER_LEFT_40_TM=60.035
PRIMER_RIGHT_40_TM=57.922
PRIMER_LEFT_40_GC_PERCENT=55.000
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=2.00
PRIMER_RIGHT_40_SELF_ANY=3.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=7.9000
PRIMER_RIGHT_40_END_STABILITY=9.9000
PRIMER_PAIR_40_COMPL_ANY=5.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=698
PRIMER_PAIR_41_PENALTY=2.143321
PRIMER_LEFT_41_PENALTY=0.035367
PRIMER_RIGHT_41_PENALTY=2.107954
PRIMER_LEFT_41_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_41_SEQUENCE=CCATCCCACACATCTTCCCT
PRIMER_LEFT_41=910,20
PRIMER_RIGHT_41=1735,20
PRIMER_LEFT_41_TM=60.035
PRIMER_RIGHT_41_TM=62.108
PRIMER_LEFT_41_GC_PERCENT=55.000
PRIMER_RIGHT_41_GC_PERCENT=55.000
PRIMER_LEFT_41_SELF_ANY=2.00
PRIMER_RIGHT_41_SELF_ANY=2.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=7.9000
PRIMER_RIGHT_41_END_STABILITY=9.4000
PRIMER_PAIR_41_COMPL_ANY=6.00
PRIMER_PAIR_41_COMPL_END=3.00
PRIMER_PAIR_41_PRODUCT_SIZE=826
PRIMER_PAIR_42_PENALTY=2.162138
PRIMER_LEFT_42_PENALTY=1.941293
PRIMER_RIGHT_42_PENALTY=0.220845
PRIMER_LEFT_42_SEQUENCE=CTGGTTGTGTCGGTAAGGTC
PRIMER_RIGHT_42_SEQUENCE=ATCCCACACATCTTCCCTTG
PRIMER_LEFT_42=911,20
PRIMER_RIGHT_42=1733,20
PRIMER_LEFT_42_TM=58.059
PRIMER_RIGHT_42_TM=59.779
PRIMER_LEFT_42_GC_PERCENT=55.000
PRIMER_RIGHT_42_GC_PERCENT=50.000
PRIMER_LEFT_42_SELF_ANY=2.00
PRIMER_RIGHT_42_SELF_ANY=2.00
PRIMER_LEFT_42_SELF_END=1.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=7.6000
PRIMER_RIGHT_42_END_STABILITY=8.5000
PRIMER_PAIR_42_COMPL_ANY=6.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=823
PRIMER_PAIR_43_PENALTY=2.162138
PRIMER_LEFT_43_PENALTY=1.941293
PRIMER_RIGHT_43_PENALTY=0.220845
PRIMER_LEFT_43_SEQUENCE=CTGGTTGTGTCGGTAAGGTC
PRIMER_RIGHT_43_SEQUENCE=CATCCCACACATCTTCCCTT
PRIMER_LEFT_43=911,20
PRIMER_RIGHT_43=1734,20
PRIMER_LEFT_43_TM=58.059
PRIMER_RIGHT_43_TM=59.779
PRIMER_LEFT_43_GC_PERCENT=55.000
PRIMER_RIGHT_43_GC_PERCENT=50.000
PRIMER_LEFT_43_SELF_ANY=2.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=7.6000
PRIMER_RIGHT_43_END_STABILITY=9.7000
PRIMER_PAIR_43_COMPL_ANY=6.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=824
PRIMER_PAIR_44_PENALTY=2.196439
PRIMER_LEFT_44_PENALTY=1.975594
PRIMER_RIGHT_44_PENALTY=0.220845
PRIMER_LEFT_44_SEQUENCE=AGCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_44_SEQUENCE=ATCCCACACATCTTCCCTTG
PRIMER_LEFT_44=909,21
PRIMER_RIGHT_44=1733,20
PRIMER_LEFT_44_TM=60.976
PRIMER_RIGHT_44_TM=59.779
PRIMER_LEFT_44_GC_PERCENT=52.381
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=4.00
PRIMER_RIGHT_44_SELF_ANY=2.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=7.9000
PRIMER_RIGHT_44_END_STABILITY=8.5000
PRIMER_PAIR_44_COMPL_ANY=6.00
PRIMER_PAIR_44_COMPL_END=2.00
PRIMER_PAIR_44_PRODUCT_SIZE=825
PRIMER_PAIR_45_PENALTY=2.196439
PRIMER_LEFT_45_PENALTY=1.975594
PRIMER_RIGHT_45_PENALTY=0.220845
PRIMER_LEFT_45_SEQUENCE=AGCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_45_SEQUENCE=CATCCCACACATCTTCCCTT
PRIMER_LEFT_45=909,21
PRIMER_RIGHT_45=1734,20
PRIMER_LEFT_45_TM=60.976
PRIMER_RIGHT_45_TM=59.779
PRIMER_LEFT_45_GC_PERCENT=52.381
PRIMER_RIGHT_45_GC_PERCENT=50.000
PRIMER_LEFT_45_SELF_ANY=4.00
PRIMER_RIGHT_45_SELF_ANY=2.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=7.9000
PRIMER_RIGHT_45_END_STABILITY=9.7000
PRIMER_PAIR_45_COMPL_ANY=6.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=826
PRIMER_PAIR_46_PENALTY=2.200236
PRIMER_LEFT_46_PENALTY=0.035367
PRIMER_RIGHT_46_PENALTY=2.164869
PRIMER_LEFT_46_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_46_SEQUENCE=CAAACTCCACATTCCCAATC
PRIMER_LEFT_46=910,20
PRIMER_RIGHT_46=1633,20
PRIMER_LEFT_46_TM=60.035
PRIMER_RIGHT_46_TM=57.835
PRIMER_LEFT_46_GC_PERCENT=55.000
PRIMER_RIGHT_46_GC_PERCENT=45.000
PRIMER_LEFT_46_SELF_ANY=2.00
PRIMER_RIGHT_46_SELF_ANY=3.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=7.9000
PRIMER_RIGHT_46_END_STABILITY=6.9000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=724
PRIMER_PAIR_47_PENALTY=2.223978
PRIMER_LEFT_47_PENALTY=0.170871
PRIMER_RIGHT_47_PENALTY=2.053107
PRIMER_LEFT_47_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_47_SEQUENCE=TGGCGACAAACTCCACATTC
PRIMER_LEFT_47=909,20
PRIMER_RIGHT_47=1639,20
PRIMER_LEFT_47_TM=60.171
PRIMER_RIGHT_47_TM=62.053
PRIMER_LEFT_47_GC_PERCENT=55.000
PRIMER_RIGHT_47_GC_PERCENT=50.000
PRIMER_LEFT_47_SELF_ANY=4.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=7.5000
PRIMER_RIGHT_47_END_STABILITY=6.9000
PRIMER_PAIR_47_COMPL_ANY=6.00
PRIMER_PAIR_47_COMPL_END=1.00
PRIMER_PAIR_47_PRODUCT_SIZE=731
PRIMER_PAIR_48_PENALTY=2.245788
PRIMER_LEFT_48_PENALTY=0.035367
PRIMER_RIGHT_48_PENALTY=2.210422
PRIMER_LEFT_48_SEQUENCE=GCTGGTTGTGTCGGTAAGGT
PRIMER_RIGHT_48_SEQUENCE=ACCAACCCACATGCGTTACA
PRIMER_LEFT_48=910,20
PRIMER_RIGHT_48=1480,20
PRIMER_LEFT_48_TM=60.035
PRIMER_RIGHT_48_TM=62.210
PRIMER_LEFT_48_GC_PERCENT=55.000
PRIMER_RIGHT_48_GC_PERCENT=50.000
PRIMER_LEFT_48_SELF_ANY=2.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=1.00
PRIMER_LEFT_48_END_STABILITY=7.9000
PRIMER_RIGHT_48_END_STABILITY=6.0000
PRIMER_PAIR_48_COMPL_ANY=5.00
PRIMER_PAIR_48_COMPL_END=2.00
PRIMER_PAIR_48_PRODUCT_SIZE=571
PRIMER_PAIR_49_PENALTY=2.249167
PRIMER_LEFT_49_PENALTY=0.170871
PRIMER_RIGHT_49_PENALTY=2.078295
PRIMER_LEFT_49_SEQUENCE=AGCTGGTTGTGTCGGTAAGG
PRIMER_RIGHT_49_SEQUENCE=ACCGAGATGATAAGTGCGAG
PRIMER_LEFT_49=909,20
PRIMER_RIGHT_49=1607,20
PRIMER_LEFT_49_TM=60.171
PRIMER_RIGHT_49_TM=57.922
PRIMER_LEFT_49_GC_PERCENT=55.000
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=4.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=7.5000
PRIMER_RIGHT_49_END_STABILITY=9.9000
PRIMER_PAIR_49_COMPL_ANY=5.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=699
=
SEQUENCE_ID=MAGIC.175_3_7912011_7912449_mt-0_sf-2_44697_80066_TRUE
SEQUENCE_TARGET=950,538
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATAAGAGCAGTNNNNNNNNNNNNNNNNNNNNGGNAAAAAAAAAAAAGGGAAANNNNNNNNGAAAAAAATAAAAAATAATGTAAAAAGAGNGGTGAAAAAAGTNATAATTGAAAAGAAAGAAAAAAGATGATAAGAGCAATTCCACATTGGCAGCTAACTGTGGACCCCACACTTCTCGGAAGTTCCGTGACCGCGACTTGAGTGAATAAGGTCCCACATGCATTTCCCTCTCTTCCCATCTCAGCCGTTTAATCTTTTCTAAATCGAACGGTTCAGATCAACGGGTCGGGTGCCAACTCAACGCCTTTTTTAGTTTTTATTTTATTTTATNTTTTTTTTTTGGATGATGTTTTCTTCTGCCAAGTCAGATTGTTTAAAAACCAAAAATAGAAGAAAATAAANTACTAATATAATATAAAACNATCTAACGGTTGTGAGAGCATTTAGCTNATTGGCAACAAATGGTATTGGCCGTTGATTTGTAATTNATGAGTTAAATTATTTTAGACTATGGTAGGACCTGTTTCCTTACTTTATAGGTTTATTGTTTAGCTTACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTTGAANAAAAAAAAATAGTTAGTGGCAAGTAAAAAACTATTTAGCCATTTGATCTTAATGGAGAAAAGNAAAAAAAAAAANGATGAAGGTAGAGAAGTGGAACCTGATGTGATTAATATTACCGGTTGNATCGGTTTGTATAGGTGGTCACAAAGATTCCATTCTGATCGTAGATTGCCTTTACCAAGGTCTCTGTCTACCGGTAATAGCCGGTGATACATCTTCTCAAGACACATTTTGAATTTTAATAATTAAAATAACATCATATTTGTGCGTGTATATGTCAGTATTTTTGTTTTCTTTTCACACGAGTCATCGCGATTAGTGGACAACAGTACACTACCCCAGGAGCCTTTTTATTGCACAATATANTATTGATGATTANTTTTAGATTTAAATATTATTATCTTAATCTTTCCGACTTTCAATATATTTTATTGTTTNTTTATTTGTTTGTTTATTTCTAGACAGTGCAAAGTCAATAAAATATTGGTANCGATAATATAGACGGAAGCGTGTGATGTCAAACTCTTGACACGTGGCAGCTTCTGTTTAATAACTGGTAACAGTCCAAAATAATTTTTTCACATGAATCCGACTCCGAAAATAATATTCTTTTNAAACCGAAAAATTAAATGTTATCAAATACTAANATATNATAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAAAGAATACGTTTTTTAATNNNNNNNNNNNNNNNTAATCGGTGGTAAAATAATTAATAAAAGAAGANATATCGGTTATTTATTATTATATGCGAGATCTCTTATAACGGAGGGCCAAGATTTTGTCACGCTGGTATATAGCGTATCGTGACCGTTGGATGTATTCACACCGANGGTGAAGATTGAGAAAATGTTTTACGGCAGGAGATATGAGCCGTGGATTTATTCGCTACTCTGCTTTTATGAGGAGCACAAATAAAGGAGATAATGGGAAGCAAGTAGAGAGATAGAGGAGAGAGAGTTTGATAAAGAGAGAGAAAAAGGGGTTGCGATTTGGGAGAAGAGAAATAGAGAGGAGAAGCATTAGNNNAGAAGAAGAANAAGATGGATAGAAATAGAGAAGCTAGAAGAGTACCTATGGCGGCTGCCGGAAATGGTCTTTCCCGGCGGAGACATAGAGCCGGAAGTTTCAGAGATTCACCAGGTTTGTTTTTGAATCTCTTGATTTGGTTTGTTTTTCATCAGATCTGAGAGAAAACGTTTGGTTCTGATTTTTCTGAACGTTTTTTTCACAGAGGAGGAAGGTCCGGTGGAGTTACCGGAGGCGGCGAGGTTGAGAGATCGAGGAGGGAGTAATAAGAAGGATCGAGAT
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=5.360163
PRIMER_LEFT_0_PENALTY=4.502955
PRIMER_RIGHT_0_PENALTY=0.857208
PRIMER_LEFT_0_SEQUENCE=TGGTAGGACCTGTTTCCTTACTTT
PRIMER_RIGHT_0_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_0=609,24
PRIMER_RIGHT_0=1595,20
PRIMER_LEFT_0_TM=59.497
PRIMER_RIGHT_0_TM=59.143
PRIMER_LEFT_0_GC_PERCENT=41.667
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=8.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=987
PRIMER_PAIR_1_PENALTY=5.584961
PRIMER_LEFT_1_PENALTY=4.727753
PRIMER_RIGHT_1_PENALTY=0.857208
PRIMER_LEFT_1_SEQUENCE=TGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_1_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_1=609,23
PRIMER_RIGHT_1=1595,20
PRIMER_LEFT_1_TM=58.272
PRIMER_RIGHT_1_TM=59.143
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=987
PRIMER_PAIR_2_PENALTY=5.674402
PRIMER_LEFT_2_PENALTY=4.502955
PRIMER_RIGHT_2_PENALTY=1.171447
PRIMER_LEFT_2_SEQUENCE=TGGTAGGACCTGTTTCCTTACTTT
PRIMER_RIGHT_2_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_2=609,24
PRIMER_RIGHT_2=1596,21
PRIMER_LEFT_2_TM=59.497
PRIMER_RIGHT_2_TM=60.171
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=988
PRIMER_PAIR_3_PENALTY=5.697731
PRIMER_LEFT_3_PENALTY=4.840523
PRIMER_RIGHT_3_PENALTY=0.857208
PRIMER_LEFT_3_SEQUENCE=ATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_3_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_3=608,20
PRIMER_RIGHT_3=1595,20
PRIMER_LEFT_3_TM=55.159
PRIMER_RIGHT_3_TM=59.143
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=988
PRIMER_PAIR_4_PENALTY=5.848470
PRIMER_LEFT_4_PENALTY=4.991262
PRIMER_RIGHT_4_PENALTY=0.857208
PRIMER_LEFT_4_SEQUENCE=GACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_4_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_4=604,24
PRIMER_RIGHT_4=1595,20
PRIMER_LEFT_4_TM=59.009
PRIMER_RIGHT_4_TM=59.143
PRIMER_LEFT_4_GC_PERCENT=45.833
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=992
PRIMER_PAIR_5_PENALTY=5.899200
PRIMER_LEFT_5_PENALTY=4.727753
PRIMER_RIGHT_5_PENALTY=1.171447
PRIMER_LEFT_5_SEQUENCE=TGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_5_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_5=609,23
PRIMER_RIGHT_5=1596,21
PRIMER_LEFT_5_TM=58.272
PRIMER_RIGHT_5_TM=60.171
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=988
PRIMER_PAIR_6_PENALTY=5.936365
PRIMER_LEFT_6_PENALTY=5.079157
PRIMER_RIGHT_6_PENALTY=0.857208
PRIMER_LEFT_6_SEQUENCE=TGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_6_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_6=609,22
PRIMER_RIGHT_6=1595,20
PRIMER_LEFT_6_TM=56.921
PRIMER_RIGHT_6_TM=59.143
PRIMER_LEFT_6_GC_PERCENT=45.455
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=987
PRIMER_PAIR_7_PENALTY=6.002864
PRIMER_LEFT_7_PENALTY=5.145656
PRIMER_RIGHT_7_PENALTY=0.857208
PRIMER_LEFT_7_SEQUENCE=AGACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_7_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_7=603,25
PRIMER_RIGHT_7=1595,20
PRIMER_LEFT_7_TM=59.854
PRIMER_RIGHT_7_TM=59.143
PRIMER_LEFT_7_GC_PERCENT=44.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=993
PRIMER_PAIR_8_PENALTY=6.011970
PRIMER_LEFT_8_PENALTY=4.840523
PRIMER_RIGHT_8_PENALTY=1.171447
PRIMER_LEFT_8_SEQUENCE=ATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_8_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_8=608,20
PRIMER_RIGHT_8=1596,21
PRIMER_LEFT_8_TM=55.159
PRIMER_RIGHT_8_TM=60.171
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=47.619
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PAIR_8_PRODUCT_SIZE=989
PRIMER_PAIR_9_PENALTY=6.111095
PRIMER_LEFT_9_PENALTY=5.253887
PRIMER_RIGHT_9_PENALTY=0.857208
PRIMER_LEFT_9_SEQUENCE=ATGGTAGGACCTGTTTCCTTACTTT
PRIMER_RIGHT_9_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_9=608,25
PRIMER_RIGHT_9=1595,20
PRIMER_LEFT_9_TM=59.746
PRIMER_RIGHT_9_TM=59.143
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PAIR_9_PRODUCT_SIZE=988
PRIMER_PAIR_10_PENALTY=6.124973
PRIMER_LEFT_10_PENALTY=5.267764
PRIMER_RIGHT_10_PENALTY=0.857208
PRIMER_LEFT_10_SEQUENCE=AGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_10_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_10=603,23
PRIMER_RIGHT_10=1595,20
PRIMER_LEFT_10_TM=57.732
PRIMER_RIGHT_10_TM=59.143
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PAIR_10_PRODUCT_SIZE=993
PRIMER_PAIR_11_PENALTY=6.150076
PRIMER_LEFT_11_PENALTY=5.292868
PRIMER_RIGHT_11_PENALTY=0.857208
PRIMER_LEFT_11_SEQUENCE=GACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_11_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_11=604,22
PRIMER_RIGHT_11=1595,20
PRIMER_LEFT_11_TM=56.707
PRIMER_RIGHT_11_TM=59.143
PRIMER_LEFT_11_GC_PERCENT=50.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PAIR_11_PRODUCT_SIZE=992
PRIMER_PAIR_12_PENALTY=6.162709
PRIMER_LEFT_12_PENALTY=4.991262
PRIMER_RIGHT_12_PENALTY=1.171447
PRIMER_LEFT_12_SEQUENCE=GACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_12_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_12=604,24
PRIMER_RIGHT_12=1596,21
PRIMER_LEFT_12_TM=59.009
PRIMER_RIGHT_12_TM=60.171
PRIMER_LEFT_12_GC_PERCENT=45.833
PRIMER_RIGHT_12_GC_PERCENT=47.619
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PAIR_12_PRODUCT_SIZE=993
PRIMER_PAIR_13_PENALTY=6.250604
PRIMER_LEFT_13_PENALTY=5.079157
PRIMER_RIGHT_13_PENALTY=1.171447
PRIMER_LEFT_13_SEQUENCE=TGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_13_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_13=609,22
PRIMER_RIGHT_13=1596,21
PRIMER_LEFT_13_TM=56.921
PRIMER_RIGHT_13_TM=60.171
PRIMER_LEFT_13_GC_PERCENT=45.455
PRIMER_RIGHT_13_GC_PERCENT=47.619
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PAIR_13_PRODUCT_SIZE=988
PRIMER_PAIR_14_PENALTY=6.267080
PRIMER_LEFT_14_PENALTY=5.409871
PRIMER_RIGHT_14_PENALTY=0.857208
PRIMER_LEFT_14_SEQUENCE=ATGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_14_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_14=608,24
PRIMER_RIGHT_14=1595,20
PRIMER_LEFT_14_TM=58.590
PRIMER_RIGHT_14_TM=59.143
PRIMER_LEFT_14_GC_PERCENT=41.667
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=5.7000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=988
PRIMER_PAIR_15_PENALTY=6.317103
PRIMER_LEFT_15_PENALTY=5.145656
PRIMER_RIGHT_15_PENALTY=1.171447
PRIMER_LEFT_15_SEQUENCE=AGACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_15_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_15=603,25
PRIMER_RIGHT_15=1596,21
PRIMER_LEFT_15_TM=59.854
PRIMER_RIGHT_15_TM=60.171
PRIMER_LEFT_15_GC_PERCENT=44.000
PRIMER_RIGHT_15_GC_PERCENT=47.619
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=994
PRIMER_PAIR_16_PENALTY=6.405639
PRIMER_LEFT_16_PENALTY=5.548430
PRIMER_RIGHT_16_PENALTY=0.857208
PRIMER_LEFT_16_SEQUENCE=CTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_16_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_16=606,22
PRIMER_RIGHT_16=1595,20
PRIMER_LEFT_16_TM=56.452
PRIMER_RIGHT_16_TM=59.143
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PAIR_16_PRODUCT_SIZE=990
PRIMER_PAIR_17_PENALTY=6.425334
PRIMER_LEFT_17_PENALTY=5.253887
PRIMER_RIGHT_17_PENALTY=1.171447
PRIMER_LEFT_17_SEQUENCE=ATGGTAGGACCTGTTTCCTTACTTT
PRIMER_RIGHT_17_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_17=608,25
PRIMER_RIGHT_17=1596,21
PRIMER_LEFT_17_TM=59.746
PRIMER_RIGHT_17_TM=60.171
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=47.619
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PAIR_17_PRODUCT_SIZE=989
PRIMER_PAIR_18_PENALTY=6.439211
PRIMER_LEFT_18_PENALTY=5.267764
PRIMER_RIGHT_18_PENALTY=1.171447
PRIMER_LEFT_18_SEQUENCE=AGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_18_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_18=603,23
PRIMER_RIGHT_18=1596,21
PRIMER_LEFT_18_TM=57.732
PRIMER_RIGHT_18_TM=60.171
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PAIR_18_PRODUCT_SIZE=994
PRIMER_PAIR_19_PENALTY=6.464315
PRIMER_LEFT_19_PENALTY=5.292868
PRIMER_RIGHT_19_PENALTY=1.171447
PRIMER_LEFT_19_SEQUENCE=GACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_19_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_19=604,22
PRIMER_RIGHT_19=1596,21
PRIMER_LEFT_19_TM=56.707
PRIMER_RIGHT_19_TM=60.171
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=47.619
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PAIR_19_PRODUCT_SIZE=993
PRIMER_PAIR_20_PENALTY=6.505402
PRIMER_LEFT_20_PENALTY=5.648193
PRIMER_RIGHT_20_PENALTY=0.857208
PRIMER_LEFT_20_SEQUENCE=TATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_20_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_20=607,21
PRIMER_RIGHT_20=1595,20
PRIMER_LEFT_20_TM=55.352
PRIMER_RIGHT_20_TM=59.143
PRIMER_LEFT_20_GC_PERCENT=42.857
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=8.2000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PAIR_20_PRODUCT_SIZE=989
PRIMER_PAIR_21_PENALTY=6.536707
PRIMER_LEFT_21_PENALTY=5.679499
PRIMER_RIGHT_21_PENALTY=0.857208
PRIMER_LEFT_21_SEQUENCE=ATGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_21_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_21=608,23
PRIMER_RIGHT_21=1595,20
PRIMER_LEFT_21_TM=57.321
PRIMER_RIGHT_21_TM=59.143
PRIMER_LEFT_21_GC_PERCENT=43.478
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=1.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=5.7000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=988
PRIMER_PAIR_22_PENALTY=6.536707
PRIMER_LEFT_22_PENALTY=5.679499
PRIMER_RIGHT_22_PENALTY=0.857208
PRIMER_LEFT_22_SEQUENCE=ACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_22_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_22=605,23
PRIMER_RIGHT_22=1595,20
PRIMER_LEFT_22_TM=57.321
PRIMER_RIGHT_22_TM=59.143
PRIMER_LEFT_22_GC_PERCENT=43.478
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=8.2000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=991
PRIMER_PAIR_23_PENALTY=6.581318
PRIMER_LEFT_23_PENALTY=5.409871
PRIMER_RIGHT_23_PENALTY=1.171447
PRIMER_LEFT_23_SEQUENCE=ATGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_23_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_23=608,24
PRIMER_RIGHT_23=1596,21
PRIMER_LEFT_23_TM=58.590
PRIMER_RIGHT_23_TM=60.171
PRIMER_LEFT_23_GC_PERCENT=41.667
PRIMER_RIGHT_23_GC_PERCENT=47.619
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=6.00
PRIMER_LEFT_23_SELF_END=1.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=5.7000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=989
PRIMER_PAIR_24_PENALTY=6.719877
PRIMER_LEFT_24_PENALTY=5.548430
PRIMER_RIGHT_24_PENALTY=1.171447
PRIMER_LEFT_24_SEQUENCE=CTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_24_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_24=606,22
PRIMER_RIGHT_24=1596,21
PRIMER_LEFT_24_TM=56.452
PRIMER_RIGHT_24_TM=60.171
PRIMER_LEFT_24_GC_PERCENT=45.455
PRIMER_RIGHT_24_GC_PERCENT=47.619
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_LEFT_24_SELF_END=1.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=8.2000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=991
PRIMER_PAIR_25_PENALTY=6.806966
PRIMER_LEFT_25_PENALTY=5.949758
PRIMER_RIGHT_25_PENALTY=0.857208
PRIMER_LEFT_25_SEQUENCE=TTAGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_25_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_25=601,25
PRIMER_RIGHT_25=1595,20
PRIMER_LEFT_25_TM=59.050
PRIMER_RIGHT_25_TM=59.143
PRIMER_LEFT_25_GC_PERCENT=44.000
PRIMER_RIGHT_25_GC_PERCENT=50.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=6.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.5000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=0.00
PRIMER_PAIR_25_PRODUCT_SIZE=995
PRIMER_PAIR_26_PENALTY=6.819640
PRIMER_LEFT_26_PENALTY=5.648193
PRIMER_RIGHT_26_PENALTY=1.171447
PRIMER_LEFT_26_SEQUENCE=TATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_26_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_26=607,21
PRIMER_RIGHT_26=1596,21
PRIMER_LEFT_26_TM=55.352
PRIMER_RIGHT_26_TM=60.171
PRIMER_LEFT_26_GC_PERCENT=42.857
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=6.00
PRIMER_LEFT_26_SELF_END=1.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PAIR_26_PRODUCT_SIZE=990
PRIMER_PAIR_27_PENALTY=6.823332
PRIMER_LEFT_27_PENALTY=5.966124
PRIMER_RIGHT_27_PENALTY=0.857208
PRIMER_LEFT_27_SEQUENCE=GGTAGGACCTGTTTCCTTACTTT
PRIMER_RIGHT_27_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_27=610,23
PRIMER_RIGHT_27=1595,20
PRIMER_LEFT_27_TM=57.034
PRIMER_RIGHT_27_TM=59.143
PRIMER_LEFT_27_GC_PERCENT=43.478
PRIMER_RIGHT_27_GC_PERCENT=50.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PAIR_27_PRODUCT_SIZE=986
PRIMER_PAIR_28_PENALTY=6.850946
PRIMER_LEFT_28_PENALTY=5.679499
PRIMER_RIGHT_28_PENALTY=1.171447
PRIMER_LEFT_28_SEQUENCE=ATGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_28_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_28=608,23
PRIMER_RIGHT_28=1596,21
PRIMER_LEFT_28_TM=57.321
PRIMER_RIGHT_28_TM=60.171
PRIMER_LEFT_28_GC_PERCENT=43.478
PRIMER_RIGHT_28_GC_PERCENT=47.619
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=5.7000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=1.00
PRIMER_PAIR_28_PRODUCT_SIZE=989
PRIMER_PAIR_29_PENALTY=6.850946
PRIMER_LEFT_29_PENALTY=5.679499
PRIMER_RIGHT_29_PENALTY=1.171447
PRIMER_LEFT_29_SEQUENCE=ACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_29_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_29=605,23
PRIMER_RIGHT_29=1596,21
PRIMER_LEFT_29_TM=57.321
PRIMER_RIGHT_29_TM=60.171
PRIMER_LEFT_29_GC_PERCENT=43.478
PRIMER_RIGHT_29_GC_PERCENT=47.619
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=1.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=8.2000
PRIMER_RIGHT_29_END_STABILITY=8.2000
PRIMER_PAIR_29_COMPL_ANY=3.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PAIR_29_PRODUCT_SIZE=992
PRIMER_PAIR_30_PENALTY=6.989451
PRIMER_LEFT_30_PENALTY=6.132243
PRIMER_RIGHT_30_PENALTY=0.857208
PRIMER_LEFT_30_SEQUENCE=TAGACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_30_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_30=602,26
PRIMER_RIGHT_30=1595,20
PRIMER_LEFT_30_TM=59.868
PRIMER_RIGHT_30_TM=59.143
PRIMER_LEFT_30_GC_PERCENT=42.308
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=8.2000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=0.00
PRIMER_PAIR_30_PRODUCT_SIZE=994
PRIMER_PAIR_31_PENALTY=7.029452
PRIMER_LEFT_31_PENALTY=6.172244
PRIMER_RIGHT_31_PENALTY=0.857208
PRIMER_LEFT_31_SEQUENCE=TTTAGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_31_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_31=600,26
PRIMER_RIGHT_31=1595,20
PRIMER_LEFT_31_TM=60.172
PRIMER_RIGHT_31_TM=59.143
PRIMER_LEFT_31_GC_PERCENT=42.308
PRIMER_RIGHT_31_GC_PERCENT=50.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=6.00
PRIMER_LEFT_31_SELF_END=1.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=8.5000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=0.00
PRIMER_PAIR_31_PRODUCT_SIZE=996
PRIMER_PAIR_32_PENALTY=7.037843
PRIMER_LEFT_32_PENALTY=6.180635
PRIMER_RIGHT_32_PENALTY=0.857208
PRIMER_LEFT_32_SEQUENCE=TAGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_32_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_32=602,24
PRIMER_RIGHT_32=1595,20
PRIMER_LEFT_32_TM=57.819
PRIMER_RIGHT_32_TM=59.143
PRIMER_LEFT_32_GC_PERCENT=45.833
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=6.00
PRIMER_LEFT_32_SELF_END=1.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=8.5000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PAIR_32_PRODUCT_SIZE=994
PRIMER_PAIR_33_PENALTY=7.121205
PRIMER_LEFT_33_PENALTY=5.949758
PRIMER_RIGHT_33_PENALTY=1.171447
PRIMER_LEFT_33_SEQUENCE=TTAGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_33_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_33=601,25
PRIMER_RIGHT_33=1596,21
PRIMER_LEFT_33_TM=59.050
PRIMER_RIGHT_33_TM=60.171
PRIMER_LEFT_33_GC_PERCENT=44.000
PRIMER_RIGHT_33_GC_PERCENT=47.619
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=6.00
PRIMER_LEFT_33_SELF_END=1.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=8.5000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PAIR_33_PRODUCT_SIZE=996
PRIMER_PAIR_34_PENALTY=7.137571
PRIMER_LEFT_34_PENALTY=5.966124
PRIMER_RIGHT_34_PENALTY=1.171447
PRIMER_LEFT_34_SEQUENCE=GGTAGGACCTGTTTCCTTACTTT
PRIMER_RIGHT_34_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_34=610,23
PRIMER_RIGHT_34=1596,21
PRIMER_LEFT_34_TM=57.034
PRIMER_RIGHT_34_TM=60.171
PRIMER_LEFT_34_GC_PERCENT=43.478
PRIMER_RIGHT_34_GC_PERCENT=47.619
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.7000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PAIR_34_PRODUCT_SIZE=987
PRIMER_PAIR_35_PENALTY=7.205696
PRIMER_LEFT_35_PENALTY=6.348487
PRIMER_RIGHT_35_PENALTY=0.857208
PRIMER_LEFT_35_SEQUENCE=GGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_35_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_35=610,22
PRIMER_RIGHT_35=1595,20
PRIMER_LEFT_35_TM=55.652
PRIMER_RIGHT_35_TM=59.143
PRIMER_LEFT_35_GC_PERCENT=45.455
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=5.7000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PAIR_35_PRODUCT_SIZE=986
PRIMER_PAIR_36_PENALTY=7.213030
PRIMER_LEFT_36_PENALTY=6.355822
PRIMER_RIGHT_36_PENALTY=0.857208
PRIMER_LEFT_36_SEQUENCE=TATGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_36_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_36=607,25
PRIMER_RIGHT_36=1595,20
PRIMER_LEFT_36_TM=58.644
PRIMER_RIGHT_36_TM=59.143
PRIMER_LEFT_36_GC_PERCENT=40.000
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=5.7000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PAIR_36_PRODUCT_SIZE=989
PRIMER_PAIR_37_PENALTY=7.303690
PRIMER_LEFT_37_PENALTY=6.132243
PRIMER_RIGHT_37_PENALTY=1.171447
PRIMER_LEFT_37_SEQUENCE=TAGACTATGGTAGGACCTGTTTCCTT
PRIMER_RIGHT_37_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_37=602,26
PRIMER_RIGHT_37=1596,21
PRIMER_LEFT_37_TM=59.868
PRIMER_RIGHT_37_TM=60.171
PRIMER_LEFT_37_GC_PERCENT=42.308
PRIMER_RIGHT_37_GC_PERCENT=47.619
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=8.2000
PRIMER_RIGHT_37_END_STABILITY=8.2000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PAIR_37_PRODUCT_SIZE=995
PRIMER_PAIR_38_PENALTY=7.343691
PRIMER_LEFT_38_PENALTY=6.172244
PRIMER_RIGHT_38_PENALTY=1.171447
PRIMER_LEFT_38_SEQUENCE=TTTAGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_38_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_38=600,26
PRIMER_RIGHT_38=1596,21
PRIMER_LEFT_38_TM=60.172
PRIMER_RIGHT_38_TM=60.171
PRIMER_LEFT_38_GC_PERCENT=42.308
PRIMER_RIGHT_38_GC_PERCENT=47.619
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=6.00
PRIMER_LEFT_38_SELF_END=1.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=8.5000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PAIR_38_PRODUCT_SIZE=997
PRIMER_PAIR_39_PENALTY=7.352082
PRIMER_LEFT_39_PENALTY=6.180635
PRIMER_RIGHT_39_PENALTY=1.171447
PRIMER_LEFT_39_SEQUENCE=TAGACTATGGTAGGACCTGTTTCC
PRIMER_RIGHT_39_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_39=602,24
PRIMER_RIGHT_39=1596,21
PRIMER_LEFT_39_TM=57.819
PRIMER_RIGHT_39_TM=60.171
PRIMER_LEFT_39_GC_PERCENT=45.833
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=8.5000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PAIR_39_PRODUCT_SIZE=995
PRIMER_PAIR_40_PENALTY=7.395766
PRIMER_LEFT_40_PENALTY=6.538557
PRIMER_RIGHT_40_PENALTY=0.857208
PRIMER_LEFT_40_SEQUENCE=CTATGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_40_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_40=606,26
PRIMER_RIGHT_40=1595,20
PRIMER_LEFT_40_TM=59.461
PRIMER_RIGHT_40_TM=59.143
PRIMER_LEFT_40_GC_PERCENT=42.308
PRIMER_RIGHT_40_GC_PERCENT=50.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=6.00
PRIMER_LEFT_40_SELF_END=1.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=5.7000
PRIMER_RIGHT_40_END_STABILITY=8.2000
PRIMER_PAIR_40_COMPL_ANY=3.00
PRIMER_PAIR_40_COMPL_END=0.00
PRIMER_PAIR_40_PRODUCT_SIZE=990
PRIMER_PAIR_41_PENALTY=7.437615
PRIMER_LEFT_41_PENALTY=6.580406
PRIMER_RIGHT_41_PENALTY=0.857208
PRIMER_LEFT_41_SEQUENCE=TATGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_41_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_41=607,24
PRIMER_RIGHT_41=1595,20
PRIMER_LEFT_41_TM=57.420
PRIMER_RIGHT_41_TM=59.143
PRIMER_LEFT_41_GC_PERCENT=41.667
PRIMER_RIGHT_41_GC_PERCENT=50.000
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=6.00
PRIMER_LEFT_41_SELF_END=1.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=5.7000
PRIMER_RIGHT_41_END_STABILITY=8.2000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=0.00
PRIMER_PAIR_41_PRODUCT_SIZE=989
PRIMER_PAIR_42_PENALTY=7.519934
PRIMER_LEFT_42_PENALTY=6.348487
PRIMER_RIGHT_42_PENALTY=1.171447
PRIMER_LEFT_42_SEQUENCE=GGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_42_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_42=610,22
PRIMER_RIGHT_42=1596,21
PRIMER_LEFT_42_TM=55.652
PRIMER_RIGHT_42_TM=60.171
PRIMER_LEFT_42_GC_PERCENT=45.455
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=6.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=5.7000
PRIMER_RIGHT_42_END_STABILITY=8.2000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=1.00
PRIMER_PAIR_42_PRODUCT_SIZE=987
PRIMER_PAIR_43_PENALTY=7.527269
PRIMER_LEFT_43_PENALTY=6.355822
PRIMER_RIGHT_43_PENALTY=1.171447
PRIMER_LEFT_43_SEQUENCE=TATGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_43_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_43=607,25
PRIMER_RIGHT_43=1596,21
PRIMER_LEFT_43_TM=58.644
PRIMER_RIGHT_43_TM=60.171
PRIMER_LEFT_43_GC_PERCENT=40.000
PRIMER_RIGHT_43_GC_PERCENT=47.619
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=6.00
PRIMER_LEFT_43_SELF_END=1.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=5.7000
PRIMER_RIGHT_43_END_STABILITY=8.2000
PRIMER_PAIR_43_COMPL_ANY=3.00
PRIMER_PAIR_43_COMPL_END=1.00
PRIMER_PAIR_43_PRODUCT_SIZE=990
PRIMER_PAIR_44_PENALTY=7.534629
PRIMER_LEFT_44_PENALTY=6.677421
PRIMER_RIGHT_44_PENALTY=0.857208
PRIMER_LEFT_44_SEQUENCE=CTATGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_44_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_44=606,25
PRIMER_RIGHT_44=1595,20
PRIMER_LEFT_44_TM=58.323
PRIMER_RIGHT_44_TM=59.143
PRIMER_LEFT_44_GC_PERCENT=44.000
PRIMER_RIGHT_44_GC_PERCENT=50.000
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=6.00
PRIMER_LEFT_44_SELF_END=1.00
PRIMER_RIGHT_44_SELF_END=0.00
PRIMER_LEFT_44_END_STABILITY=5.7000
PRIMER_RIGHT_44_END_STABILITY=8.2000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=990
PRIMER_PAIR_45_PENALTY=7.710004
PRIMER_LEFT_45_PENALTY=6.538557
PRIMER_RIGHT_45_PENALTY=1.171447
PRIMER_LEFT_45_SEQUENCE=CTATGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_45_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_45=606,26
PRIMER_RIGHT_45=1596,21
PRIMER_LEFT_45_TM=59.461
PRIMER_RIGHT_45_TM=60.171
PRIMER_LEFT_45_GC_PERCENT=42.308
PRIMER_RIGHT_45_GC_PERCENT=47.619
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=6.00
PRIMER_LEFT_45_SELF_END=1.00
PRIMER_RIGHT_45_SELF_END=0.00
PRIMER_LEFT_45_END_STABILITY=5.7000
PRIMER_RIGHT_45_END_STABILITY=8.2000
PRIMER_PAIR_45_COMPL_ANY=3.00
PRIMER_PAIR_45_COMPL_END=1.00
PRIMER_PAIR_45_PRODUCT_SIZE=991
PRIMER_PAIR_46_PENALTY=7.751853
PRIMER_LEFT_46_PENALTY=6.580406
PRIMER_RIGHT_46_PENALTY=1.171447
PRIMER_LEFT_46_SEQUENCE=TATGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_46_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_46=607,24
PRIMER_RIGHT_46=1596,21
PRIMER_LEFT_46_TM=57.420
PRIMER_RIGHT_46_TM=60.171
PRIMER_LEFT_46_GC_PERCENT=41.667
PRIMER_RIGHT_46_GC_PERCENT=47.619
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=6.00
PRIMER_LEFT_46_SELF_END=1.00
PRIMER_RIGHT_46_SELF_END=0.00
PRIMER_LEFT_46_END_STABILITY=5.7000
PRIMER_RIGHT_46_END_STABILITY=8.2000
PRIMER_PAIR_46_COMPL_ANY=3.00
PRIMER_PAIR_46_COMPL_END=1.00
PRIMER_PAIR_46_PRODUCT_SIZE=990
PRIMER_PAIR_47_PENALTY=7.830113
PRIMER_LEFT_47_PENALTY=4.502955
PRIMER_RIGHT_47_PENALTY=3.327158
PRIMER_LEFT_47_SEQUENCE=TGGTAGGACCTGTTTCCTTACTTT
PRIMER_RIGHT_47_SEQUENCE=TGACATCACACGCTTCCGTC
PRIMER_LEFT_47=609,24
PRIMER_RIGHT_47=1592,20
PRIMER_LEFT_47_TM=59.497
PRIMER_RIGHT_47_TM=63.327
PRIMER_LEFT_47_GC_PERCENT=41.667
PRIMER_RIGHT_47_GC_PERCENT=55.000
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=1.00
PRIMER_LEFT_47_END_STABILITY=6.7000
PRIMER_RIGHT_47_END_STABILITY=9.6000
PRIMER_PAIR_47_COMPL_ANY=3.00
PRIMER_PAIR_47_COMPL_END=0.00
PRIMER_PAIR_47_PRODUCT_SIZE=984
PRIMER_PAIR_48_PENALTY=7.848868
PRIMER_LEFT_48_PENALTY=6.677421
PRIMER_RIGHT_48_PENALTY=1.171447
PRIMER_LEFT_48_SEQUENCE=CTATGGTAGGACCTGTTTCCTTACT
PRIMER_RIGHT_48_SEQUENCE=AGTTTGACATCACACGCTTCC
PRIMER_LEFT_48=606,25
PRIMER_RIGHT_48=1596,21
PRIMER_LEFT_48_TM=58.323
PRIMER_RIGHT_48_TM=60.171
PRIMER_LEFT_48_GC_PERCENT=44.000
PRIMER_RIGHT_48_GC_PERCENT=47.619
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=6.00
PRIMER_LEFT_48_SELF_END=1.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=5.7000
PRIMER_RIGHT_48_END_STABILITY=8.2000
PRIMER_PAIR_48_COMPL_ANY=3.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=991
PRIMER_PAIR_49_PENALTY=7.974776
PRIMER_LEFT_49_PENALTY=7.117567
PRIMER_RIGHT_49_PENALTY=0.857208
PRIMER_LEFT_49_SEQUENCE=ACTATGGTAGGACCTGTTTCCTTACTT
PRIMER_RIGHT_49_SEQUENCE=GTTTGACATCACACGCTTCC
PRIMER_LEFT_49=605,27
PRIMER_RIGHT_49=1595,20
PRIMER_LEFT_49_TM=60.118
PRIMER_RIGHT_49_TM=59.143
PRIMER_LEFT_49_GC_PERCENT=40.741
PRIMER_RIGHT_49_GC_PERCENT=50.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=6.00
PRIMER_LEFT_49_SELF_END=1.00
PRIMER_RIGHT_49_SELF_END=0.00
PRIMER_LEFT_49_END_STABILITY=5.7000
PRIMER_RIGHT_49_END_STABILITY=8.2000
PRIMER_PAIR_49_COMPL_ANY=3.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=991
=
SEQUENCE_ID=MAGIC.433_1_14191054_14191068_ct-1_oy-0_506466_701553_TRUE
SEQUENCE_TARGET=950,114
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_5_20813401_20813406_can-0_sf-2_3811_1105822_TRUE
SEQUENCE_TARGET=950,105
SEQUENCE_TEMPLATE=NTNNNTTNAGNNGNNGACTNNNNNNCTNANTNGNANANNACANTNTGTGNANNATNNNNNGGNNCTTANCNNNTNNNTTTCANNNTGANTGNTTCNTTNNTNCATATNNTATNNNCNCNATNNTNTGNCTTTCANNNTNNTNGNTTNTNGTNNATNATATCANCNNNNNTGNNNNANGNNTNNCACATTNGCTTCTGNNNNNTNATNTNNANNNNCCTGNNNNANNGACANNTTTGCNNCNGAAGTTTNNCTNNANNTNTGGNNGNNACNNNTNTNCAGNNNAANNTNNANTNCANNTNTGNTNNNANNTTGTNNTANAATNTGNANTTNNNNNANGNTCNNNNTTNNNNTGNTNNANAANNTNCNNNNNNCTGANANNNNNTTCTNANTNNTNGAGNNNNGCTTCCNNNNNNNANANNCTNATNNNANNTNNTNACTNTNTNGTNTNNNTNNNAGNNGNGANATNCTANNTGNNCTNCNGTCTTTNGNNNANTNAANTTNTANTNTNTNCNNCTTTTGNGTNAGTTNNANTTNNNNCNNCTNNNNNGTNGTNNAANNNTGGATCNANTNANNANCNAANNANATCNNTTNNNNNNCCANNNANANCAGNTTTNNNTTCTNCANNTACNNNNNNNNNNCCANATTTTNNNNNNNNGNNNNNNCANNCANNNCANAAATANNACNTATTTTNACTCNTCCNNANTNNCNANTANACNNTNATNTTNNNNNNCNTCNNTACNNTCNNTNNTNNNACTNTNNNNNNNNGANGTNGCNNNNNAGCACNNNNNNNTNGNTNCCNTCNNNNGGTNTNNNNNTNCNACNNTNNCNNNNNNAAGNGNANCNGACTCNNATTAGNTTNATNANNNNATNTANNCNNNNTTNTCTNTGNNNTCAANTNTNTNNNTNTNNNNGTTTTNTNNNNNAATNTNANCNNCCTNNATGCNANANNATTNTTNTNTNGNNGNNTNTNCCNNNNNTGTTNNNNNANCNNNTACNNTGNNANNTNTNNTTCTATTCCATCATGCGGNAAGTTGTTCACTCAGCAGATACAGTAATTTTTAGTTCCTGTTTTCCTGCTACGCTCTAGNCATGTGCAAAATGATAATTGCCTATATGCAATATTTCTTTTGGCTGGTTCTCGGTCAATGAAAAAGTGATAATCTGCATTTATGCACTACTTCTTTTTATTGTGACCCTTTAGAATTCATGTAGGTAGTTACTTTCTTGCTCTATTCATGTAGATAGTTACTTTCTTGCTATGNCTTTCAGAAGCAAATTCTGTTTTGCTAAGCTGTCAAGTCNTATTTGGTTTCTNCTCCCTGTCATTGTGGAGAGATTAAGATGGTGGCACTTTTTTCTCTCTTGCTTCTTTTGTAATAGTTTTTGTTNNNNNNANGTGTTCCTTCAAATNACAGATTANTAATCTATTACAATCCTACATAGTCGTTTTNGCATACTGATATCATTTCTCATTTTCTCAATCTCTACCTTTCCCAGAGGGCGTGGCTATTAAAGCTCCTAGCTATTGCACTGCATACTGGTTCTGGAAGCAGTTCAGCTCACCTAGAGGCATGTCAGAGCATACTTTCTCATCTTTTTGGGCGGGAGGTAACTGAAGCAGCGANTGAACCTTTTTCTTCCAGTACATATCCTCAGGATGGTCTTGATTATGCTGGAACTAGTTCAATCAGTAAAAGTAANGTGAGTTTGGAAACACCTGCGTTTTATGCTCTACTATACTTTGGCACATCCTAGTTTTATCCCTACCCCTGCCGGNGTTTTGCATGTGTAGCATTGGTCTAAGAGTCTACCATTTTATTTTTGAGGCGATCCATGCATGATACAAATAACTGTACTGACCCCACTCTTTCTAAACTCACCAGCATGTTGCCTTGCAGGGTGATGTGCACCATCTCTTAACAGTTTGACATTTATTGAACAGCAGTATTTAAAATATTAAGATGTCTCCTTTTTCATGCAGCAGCATTTTCTTTCACTGTAAATC
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.149_3_9592726_9592869_wil-2_ct-1_492475_19504_TRUE
SEQUENCE_TARGET=950,243
SEQUENCE_TEMPLATE=TNAAATTNAANCTANAGCACAAAAGCANNATTATATCGGTCTTGTTGGAAAGATTTGATNATNCAAAAAATTGGACTATTCTTTATAGTCCACTTGGTTAAATAATTNGTGGACATGCATGCATGNTGGTACTNGGTACGATTATGNNTGNATTAAAANCGACCATAGNCATACAACAAATAGNCANAAATGCGANGACCACATTTTCCTAGTNTATCTGANAACTTTAAGCCGCCGTTCACTGTTGAATACAANNAANTATTATNTTTAAGGNNNNNAATAAAGCGTTTTGTATGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNAAATTGACTGTTCGTNACNAAAATNTAGAAACCATATACGAAANAAGGTTTGCGATCATTTTTTNTGNNTTTTTTTTGTAAACCGCATGAGTTNCAANNTTTTNACTCATTTTCNNTGTCCGGNNCTAGTNTATANTCCATTCNAANTTAAAGATGTTCCTNTNTCACAAAGAGCCATNNAATCCGGNGTACNAGAGAACCAAANCCCTTGTACTANTACCACTTGGCCANCACTGCCANNACCACCTCTTNGGTAGNTTTTGNGGTCNTTCATGATTNATTTNTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTATTANTACTACTNAANCCAGTCATGNTCAANTNTCCCTTNCAATGNTACAATGTTTTCTAGTCTACNANGTTATTTTTCNTACTGCNTTCATGANTNCAACTTTGAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANAATAGTNNTATGGATAGCATCTTCTTNNNTGTCTCATGTCNCNTCTNTTTTTNTTTGNTAAAAGGTTATCTTGTCGTCTCTCTCTTNATTTNTTTGTTGNNGCNGGCGTTNGCAAGTCNACTANNNNNTATCCGNCACNNAAAAANNACATCCGTACATGATAANAANTTTGAATTNAANATGTNAAAANTCNANAATTTCAAATNTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAATGNGGGATATNNNNNNTAGGAGTTGGAACNAAANCTNCAAACTNAAAGGCCATTGATNGCTNAANAGAAAGCCCAANAACTAAGATAGTANTGGAGANGCAAATATTNGCCAACTTNAATTATAGCCCATTTTGGCAGTTGTACGCCCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTACATGCNTTTNTTANTATATGNTATGNTCANATGTAGAANTAGAATATANAACANCCGGNCNACAAACCAAATTAACACTACTTCATTATACAATNAANCATGAGAAAANNANAGAAAGNATTCTNNCTTATTTTAAGATGATTATANTTTTGNTTCATTGACGACGAGTAGGCACAAGTTCAAGNGANACTTTCTTNCCAATAATAATCGCACCTTCTTCTTCCAAATCGATATCTTTCACTGTCCTTCCTTCAGGCAATCCCCAATCAAAGAAGTAAAGNAAATTCAACAATCCTAATTCGACTNTNGCGATNCCCATNGTCATACCGGGACAAATCCTTCTACCAGAACCAAACGGNANNAGCTCGAAGTTAAGTCCTCTNTAATCAACAGAACTATCAACGAATCTTTCCGGTNTAAACTCTTCTGGATTCTCCCAGAGGTCAGGATCNCGTCCTATCGCGTAAACATTGACCATGATCTGAGTTTTCTNTGGAATGTCATAGCCTTGGATCTTGATTTTAGCCATTGCCTCTCTTGGTAATAAAAGTGGAGCTGCTGGGTGTAATCTNAATGTCTCTTTGATCACTAGCTTGAAGTAGTTNAGTTGGTTAAGATCTTGTTCTGTGATTCTNTCTCTTTTTTCACCAAGTACTGTCCTAACCTCATCTTGCACTTTCTTCATCACTCTTGG
PRIMER_LEFT_NUM_RETURNED=50
PRIMER_RIGHT_NUM_RETURNED=50
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=50
PRIMER_PAIR_0_PENALTY=6.584750
PRIMER_LEFT_0_PENALTY=6.398149
PRIMER_RIGHT_0_PENALTY=0.186600
PRIMER_LEFT_0_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_0_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_0=892,25
PRIMER_RIGHT_0=1673,20
PRIMER_LEFT_0_TM=58.602
PRIMER_RIGHT_0_TM=59.813
PRIMER_LEFT_0_GC_PERCENT=40.000
PRIMER_RIGHT_0_GC_PERCENT=45.000
PRIMER_LEFT_0_SELF_ANY=5.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=0.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_LEFT_0_END_STABILITY=6.4000
PRIMER_RIGHT_0_END_STABILITY=8.6000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=782
PRIMER_PAIR_1_PENALTY=6.854581
PRIMER_LEFT_1_PENALTY=6.667981
PRIMER_RIGHT_1_PENALTY=0.186600
PRIMER_LEFT_1_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_1=893,24
PRIMER_RIGHT_1=1673,20
PRIMER_LEFT_1_TM=57.332
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=781
PRIMER_PAIR_2_PENALTY=6.917488
PRIMER_LEFT_2_PENALTY=6.730887
PRIMER_RIGHT_2_PENALTY=0.186600
PRIMER_LEFT_2_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_2_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_2=894,25
PRIMER_RIGHT_2=1673,20
PRIMER_LEFT_2_TM=58.269
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=780
PRIMER_PAIR_3_PENALTY=6.917488
PRIMER_LEFT_3_PENALTY=6.730887
PRIMER_RIGHT_3_PENALTY=0.186600
PRIMER_LEFT_3_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_3_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_3=893,25
PRIMER_RIGHT_3=1673,20
PRIMER_LEFT_3_TM=58.269
PRIMER_RIGHT_3_TM=59.813
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=781
PRIMER_PAIR_4_PENALTY=6.926245
PRIMER_LEFT_4_PENALTY=6.730887
PRIMER_RIGHT_4_PENALTY=0.195358
PRIMER_LEFT_4_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_4_SEQUENCE=GATTGCCTGAAGGAAGGACA
PRIMER_LEFT_4=894,25
PRIMER_RIGHT_4=1709,20
PRIMER_LEFT_4_TM=58.269
PRIMER_RIGHT_4_TM=60.195
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=816
PRIMER_PAIR_5_PENALTY=6.926245
PRIMER_LEFT_5_PENALTY=6.730887
PRIMER_RIGHT_5_PENALTY=0.195358
PRIMER_LEFT_5_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_5_SEQUENCE=GATTGCCTGAAGGAAGGACA
PRIMER_LEFT_5=893,25
PRIMER_RIGHT_5=1709,20
PRIMER_LEFT_5_TM=58.269
PRIMER_RIGHT_5_TM=60.195
PRIMER_LEFT_5_GC_PERCENT=40.000
PRIMER_RIGHT_5_GC_PERCENT=50.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PAIR_5_PRODUCT_SIZE=817
PRIMER_PAIR_6_PENALTY=7.114275
PRIMER_LEFT_6_PENALTY=6.398149
PRIMER_RIGHT_6_PENALTY=0.716126
PRIMER_LEFT_6_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_6_SEQUENCE=ATTGCCTGAAGGAAGGACAG
PRIMER_LEFT_6=892,25
PRIMER_RIGHT_6=1708,20
PRIMER_LEFT_6_TM=58.602
PRIMER_RIGHT_6_TM=59.284
PRIMER_LEFT_6_GC_PERCENT=40.000
PRIMER_RIGHT_6_GC_PERCENT=50.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=817
PRIMER_PAIR_7_PENALTY=7.116643
PRIMER_LEFT_7_PENALTY=6.730887
PRIMER_RIGHT_7_PENALTY=0.385756
PRIMER_LEFT_7_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_7_SEQUENCE=GCCTGAAGGAAGGACAGTGA
PRIMER_LEFT_7=893,25
PRIMER_RIGHT_7=1705,20
PRIMER_LEFT_7_TM=58.269
PRIMER_RIGHT_7_TM=60.386
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PAIR_7_PRODUCT_SIZE=813
PRIMER_PAIR_8_PENALTY=7.153690
PRIMER_LEFT_8_PENALTY=6.398149
PRIMER_RIGHT_8_PENALTY=0.755541
PRIMER_LEFT_8_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_8_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_8=892,25
PRIMER_RIGHT_8=1790,20
PRIMER_LEFT_8_TM=58.602
PRIMER_RIGHT_8_TM=59.244
PRIMER_LEFT_8_GC_PERCENT=40.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=5.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PAIR_8_PRODUCT_SIZE=899
PRIMER_PAIR_9_PENALTY=7.212176
PRIMER_LEFT_9_PENALTY=7.025575
PRIMER_RIGHT_9_PENALTY=0.186600
PRIMER_LEFT_9_SEQUENCE=AGGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_9_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_9=895,24
PRIMER_RIGHT_9=1673,20
PRIMER_LEFT_9_TM=56.974
PRIMER_RIGHT_9_TM=59.813
PRIMER_LEFT_9_GC_PERCENT=41.667
PRIMER_RIGHT_9_GC_PERCENT=45.000
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PAIR_9_PRODUCT_SIZE=779
PRIMER_PAIR_10_PENALTY=7.212176
PRIMER_LEFT_10_PENALTY=7.025575
PRIMER_RIGHT_10_PENALTY=0.186600
PRIMER_LEFT_10_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_10_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_10=894,24
PRIMER_RIGHT_10=1673,20
PRIMER_LEFT_10_TM=56.974
PRIMER_RIGHT_10_TM=59.813
PRIMER_LEFT_10_GC_PERCENT=41.667
PRIMER_RIGHT_10_GC_PERCENT=45.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=8.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=780
PRIMER_PAIR_11_PENALTY=7.220933
PRIMER_LEFT_11_PENALTY=7.025575
PRIMER_RIGHT_11_PENALTY=0.195358
PRIMER_LEFT_11_SEQUENCE=AGGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_11_SEQUENCE=GATTGCCTGAAGGAAGGACA
PRIMER_LEFT_11=895,24
PRIMER_RIGHT_11=1709,20
PRIMER_LEFT_11_TM=56.974
PRIMER_RIGHT_11_TM=60.195
PRIMER_LEFT_11_GC_PERCENT=41.667
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=815
PRIMER_PAIR_12_PENALTY=7.220933
PRIMER_LEFT_12_PENALTY=7.025575
PRIMER_RIGHT_12_PENALTY=0.195358
PRIMER_LEFT_12_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_12_SEQUENCE=GATTGCCTGAAGGAAGGACA
PRIMER_LEFT_12=894,24
PRIMER_RIGHT_12=1709,20
PRIMER_LEFT_12_TM=56.974
PRIMER_RIGHT_12_TM=60.195
PRIMER_LEFT_12_GC_PERCENT=41.667
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=816
PRIMER_PAIR_13_PENALTY=7.255787
PRIMER_LEFT_13_PENALTY=7.069187
PRIMER_RIGHT_13_PENALTY=0.186600
PRIMER_LEFT_13_SEQUENCE=GGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_13_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_13=896,23
PRIMER_RIGHT_13=1673,20
PRIMER_LEFT_13_TM=55.931
PRIMER_RIGHT_13_TM=59.813
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=45.000
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PAIR_13_PRODUCT_SIZE=778
PRIMER_PAIR_14_PENALTY=7.255787
PRIMER_LEFT_14_PENALTY=7.069187
PRIMER_RIGHT_14_PENALTY=0.186600
PRIMER_LEFT_14_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_14_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_14=894,23
PRIMER_RIGHT_14=1673,20
PRIMER_LEFT_14_TM=55.931
PRIMER_RIGHT_14_TM=59.813
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=45.000
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PAIR_14_PRODUCT_SIZE=780
PRIMER_PAIR_15_PENALTY=7.260147
PRIMER_LEFT_15_PENALTY=7.069187
PRIMER_RIGHT_15_PENALTY=0.190960
PRIMER_LEFT_15_SEQUENCE=GGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_15_SEQUENCE=CTGACCTCTGGGAGAATCCA
PRIMER_LEFT_15=896,23
PRIMER_RIGHT_15=1895,20
PRIMER_LEFT_15_TM=55.931
PRIMER_RIGHT_15_TM=60.191
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PAIR_15_PRODUCT_SIZE=1000
PRIMER_PAIR_16_PENALTY=7.264544
PRIMER_LEFT_16_PENALTY=7.069187
PRIMER_RIGHT_16_PENALTY=0.195358
PRIMER_LEFT_16_SEQUENCE=GGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_16_SEQUENCE=GATTGCCTGAAGGAAGGACA
PRIMER_LEFT_16=896,23
PRIMER_RIGHT_16=1709,20
PRIMER_LEFT_16_TM=55.931
PRIMER_RIGHT_16_TM=60.195
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PAIR_16_PRODUCT_SIZE=814
PRIMER_PAIR_17_PENALTY=7.281536
PRIMER_LEFT_17_PENALTY=6.398149
PRIMER_RIGHT_17_PENALTY=0.883387
PRIMER_LEFT_17_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_17_SEQUENCE=TTTGGAAGAAGAAGGTGCGA
PRIMER_LEFT_17=892,25
PRIMER_RIGHT_17=1675,20
PRIMER_LEFT_17_TM=58.602
PRIMER_RIGHT_17_TM=60.883
PRIMER_LEFT_17_GC_PERCENT=40.000
PRIMER_RIGHT_17_GC_PERCENT=45.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=10.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=784
PRIMER_PAIR_18_PENALTY=7.368066
PRIMER_LEFT_18_PENALTY=6.398149
PRIMER_RIGHT_18_PENALTY=0.969917
PRIMER_LEFT_18_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_18_SEQUENCE=GGATTGCCTGAAGGAAGGAC
PRIMER_LEFT_18=892,25
PRIMER_RIGHT_18=1710,20
PRIMER_LEFT_18_TM=58.602
PRIMER_RIGHT_18_TM=60.970
PRIMER_LEFT_18_GC_PERCENT=40.000
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PAIR_18_PRODUCT_SIZE=819
PRIMER_PAIR_19_PENALTY=7.384107
PRIMER_LEFT_19_PENALTY=6.667981
PRIMER_RIGHT_19_PENALTY=0.716126
PRIMER_LEFT_19_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_19_SEQUENCE=ATTGCCTGAAGGAAGGACAG
PRIMER_LEFT_19=893,24
PRIMER_RIGHT_19=1708,20
PRIMER_LEFT_19_TM=57.332
PRIMER_RIGHT_19_TM=59.284
PRIMER_LEFT_19_GC_PERCENT=41.667
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=816
PRIMER_PAIR_20_PENALTY=7.411331
PRIMER_LEFT_20_PENALTY=7.025575
PRIMER_RIGHT_20_PENALTY=0.385756
PRIMER_LEFT_20_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_20_SEQUENCE=GCCTGAAGGAAGGACAGTGA
PRIMER_LEFT_20=894,24
PRIMER_RIGHT_20=1705,20
PRIMER_LEFT_20_TM=56.974
PRIMER_RIGHT_20_TM=60.386
PRIMER_LEFT_20_GC_PERCENT=41.667
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=6.4000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PAIR_20_PRODUCT_SIZE=812
PRIMER_PAIR_21_PENALTY=7.423522
PRIMER_LEFT_21_PENALTY=6.667981
PRIMER_RIGHT_21_PENALTY=0.755541
PRIMER_LEFT_21_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_21_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_21=893,24
PRIMER_RIGHT_21=1790,20
PRIMER_LEFT_21_TM=57.332
PRIMER_RIGHT_21_TM=59.244
PRIMER_LEFT_21_GC_PERCENT=41.667
PRIMER_RIGHT_21_GC_PERCENT=50.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=5.6000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PAIR_21_PRODUCT_SIZE=898
PRIMER_PAIR_22_PENALTY=7.447013
PRIMER_LEFT_22_PENALTY=6.730887
PRIMER_RIGHT_22_PENALTY=0.716126
PRIMER_LEFT_22_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_22_SEQUENCE=ATTGCCTGAAGGAAGGACAG
PRIMER_LEFT_22=893,25
PRIMER_RIGHT_22=1708,20
PRIMER_LEFT_22_TM=58.269
PRIMER_RIGHT_22_TM=59.284
PRIMER_LEFT_22_GC_PERCENT=40.000
PRIMER_RIGHT_22_GC_PERCENT=50.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=0.00
PRIMER_RIGHT_22_SELF_END=1.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=6.4000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=3.00
PRIMER_PAIR_22_PRODUCT_SIZE=816
PRIMER_PAIR_23_PENALTY=7.486429
PRIMER_LEFT_23_PENALTY=6.730887
PRIMER_RIGHT_23_PENALTY=0.755541
PRIMER_LEFT_23_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_23_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_23=894,25
PRIMER_RIGHT_23=1790,20
PRIMER_LEFT_23_TM=58.269
PRIMER_RIGHT_23_TM=59.244
PRIMER_LEFT_23_GC_PERCENT=40.000
PRIMER_RIGHT_23_GC_PERCENT=50.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=5.6000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=1.00
PRIMER_PAIR_23_PRODUCT_SIZE=897
PRIMER_PAIR_24_PENALTY=7.486429
PRIMER_LEFT_24_PENALTY=6.730887
PRIMER_RIGHT_24_PENALTY=0.755541
PRIMER_LEFT_24_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_24_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_24=893,25
PRIMER_RIGHT_24=1790,20
PRIMER_LEFT_24_TM=58.269
PRIMER_RIGHT_24_TM=59.244
PRIMER_LEFT_24_GC_PERCENT=40.000
PRIMER_RIGHT_24_GC_PERCENT=50.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=4.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.4000
PRIMER_RIGHT_24_END_STABILITY=5.6000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PAIR_24_PRODUCT_SIZE=898
PRIMER_PAIR_25_PENALTY=7.551368
PRIMER_LEFT_25_PENALTY=6.667981
PRIMER_RIGHT_25_PENALTY=0.883387
PRIMER_LEFT_25_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_25_SEQUENCE=TTTGGAAGAAGAAGGTGCGA
PRIMER_LEFT_25=893,24
PRIMER_RIGHT_25=1675,20
PRIMER_LEFT_25_TM=57.332
PRIMER_RIGHT_25_TM=60.883
PRIMER_LEFT_25_GC_PERCENT=41.667
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=0.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=10.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PAIR_25_PRODUCT_SIZE=783
PRIMER_PAIR_26_PENALTY=7.591815
PRIMER_LEFT_26_PENALTY=6.398149
PRIMER_RIGHT_26_PENALTY=1.193666
PRIMER_LEFT_26_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_26_SEQUENCE=AGAAGGATTTGTCCCGGTATG
PRIMER_LEFT_26=892,25
PRIMER_RIGHT_26=1791,21
PRIMER_LEFT_26_TM=58.602
PRIMER_RIGHT_26_TM=60.194
PRIMER_LEFT_26_GC_PERCENT=40.000
PRIMER_RIGHT_26_GC_PERCENT=47.619
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=4.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=5.6000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PAIR_26_PRODUCT_SIZE=900
PRIMER_PAIR_27_PENALTY=7.614274
PRIMER_LEFT_27_PENALTY=6.730887
PRIMER_RIGHT_27_PENALTY=0.883387
PRIMER_LEFT_27_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_27_SEQUENCE=TTTGGAAGAAGAAGGTGCGA
PRIMER_LEFT_27=893,25
PRIMER_RIGHT_27=1675,20
PRIMER_LEFT_27_TM=58.269
PRIMER_RIGHT_27_TM=60.883
PRIMER_LEFT_27_GC_PERCENT=40.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=0.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=6.4000
PRIMER_RIGHT_27_END_STABILITY=10.2000
PRIMER_PAIR_27_COMPL_ANY=4.00
PRIMER_PAIR_27_COMPL_END=3.00
PRIMER_PAIR_27_PRODUCT_SIZE=783
PRIMER_PAIR_28_PENALTY=7.637898
PRIMER_LEFT_28_PENALTY=6.667981
PRIMER_RIGHT_28_PENALTY=0.969917
PRIMER_LEFT_28_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_28_SEQUENCE=GGATTGCCTGAAGGAAGGAC
PRIMER_LEFT_28=893,24
PRIMER_RIGHT_28=1710,20
PRIMER_LEFT_28_TM=57.332
PRIMER_RIGHT_28_TM=60.970
PRIMER_LEFT_28_GC_PERCENT=41.667
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=4.00
PRIMER_LEFT_28_SELF_END=0.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=6.4000
PRIMER_RIGHT_28_END_STABILITY=7.6000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PAIR_28_PRODUCT_SIZE=818
PRIMER_PAIR_29_PENALTY=7.641739
PRIMER_LEFT_29_PENALTY=7.455139
PRIMER_RIGHT_29_PENALTY=0.186600
PRIMER_LEFT_29_SEQUENCE=AGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_29_SEQUENCE=TGGAAGAAGAAGGTGCGATT
PRIMER_LEFT_29=895,23
PRIMER_RIGHT_29=1673,20
PRIMER_LEFT_29_TM=55.545
PRIMER_RIGHT_29_TM=59.813
PRIMER_LEFT_29_GC_PERCENT=43.478
PRIMER_RIGHT_29_GC_PERCENT=45.000
PRIMER_LEFT_29_SELF_ANY=3.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=6.4000
PRIMER_RIGHT_29_END_STABILITY=8.6000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PAIR_29_PRODUCT_SIZE=779
PRIMER_PAIR_30_PENALTY=7.650496
PRIMER_LEFT_30_PENALTY=7.455139
PRIMER_RIGHT_30_PENALTY=0.195358
PRIMER_LEFT_30_SEQUENCE=AGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_30_SEQUENCE=GATTGCCTGAAGGAAGGACA
PRIMER_LEFT_30=895,23
PRIMER_RIGHT_30=1709,20
PRIMER_LEFT_30_TM=55.545
PRIMER_RIGHT_30_TM=60.195
PRIMER_LEFT_30_GC_PERCENT=43.478
PRIMER_RIGHT_30_GC_PERCENT=50.000
PRIMER_LEFT_30_SELF_ANY=3.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=3.00
PRIMER_PAIR_30_PRODUCT_SIZE=815
PRIMER_PAIR_31_PENALTY=7.700804
PRIMER_LEFT_31_PENALTY=6.730887
PRIMER_RIGHT_31_PENALTY=0.969917
PRIMER_LEFT_31_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_31_SEQUENCE=GGATTGCCTGAAGGAAGGAC
PRIMER_LEFT_31=893,25
PRIMER_RIGHT_31=1710,20
PRIMER_LEFT_31_TM=58.269
PRIMER_RIGHT_31_TM=60.970
PRIMER_LEFT_31_GC_PERCENT=40.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=5.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.6000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=3.00
PRIMER_PAIR_31_PRODUCT_SIZE=818
PRIMER_PAIR_32_PENALTY=7.741701
PRIMER_LEFT_32_PENALTY=7.025575
PRIMER_RIGHT_32_PENALTY=0.716126
PRIMER_LEFT_32_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_32_SEQUENCE=ATTGCCTGAAGGAAGGACAG
PRIMER_LEFT_32=894,24
PRIMER_RIGHT_32=1708,20
PRIMER_LEFT_32_TM=56.974
PRIMER_RIGHT_32_TM=59.284
PRIMER_LEFT_32_GC_PERCENT=41.667
PRIMER_RIGHT_32_GC_PERCENT=50.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=1.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=6.4000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=3.00
PRIMER_PAIR_32_PRODUCT_SIZE=815
PRIMER_PAIR_33_PENALTY=7.781116
PRIMER_LEFT_33_PENALTY=7.025575
PRIMER_RIGHT_33_PENALTY=0.755541
PRIMER_LEFT_33_SEQUENCE=AGGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_33_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_33=895,24
PRIMER_RIGHT_33=1790,20
PRIMER_LEFT_33_TM=56.974
PRIMER_RIGHT_33_TM=59.244
PRIMER_LEFT_33_GC_PERCENT=41.667
PRIMER_RIGHT_33_GC_PERCENT=50.000
PRIMER_LEFT_33_SELF_ANY=3.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=6.7000
PRIMER_RIGHT_33_END_STABILITY=5.6000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PAIR_33_PRODUCT_SIZE=896
PRIMER_PAIR_34_PENALTY=7.781116
PRIMER_LEFT_34_PENALTY=7.025575
PRIMER_RIGHT_34_PENALTY=0.755541
PRIMER_LEFT_34_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_34_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_34=894,24
PRIMER_RIGHT_34=1790,20
PRIMER_LEFT_34_TM=56.974
PRIMER_RIGHT_34_TM=59.244
PRIMER_LEFT_34_GC_PERCENT=41.667
PRIMER_RIGHT_34_GC_PERCENT=50.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=4.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=5.6000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=0.00
PRIMER_PAIR_34_PRODUCT_SIZE=897
PRIMER_PAIR_35_PENALTY=7.785313
PRIMER_LEFT_35_PENALTY=7.069187
PRIMER_RIGHT_35_PENALTY=0.716126
PRIMER_LEFT_35_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_35_SEQUENCE=ATTGCCTGAAGGAAGGACAG
PRIMER_LEFT_35=894,23
PRIMER_RIGHT_35=1708,20
PRIMER_LEFT_35_TM=55.931
PRIMER_RIGHT_35_TM=59.284
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=4.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=1.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=6.4000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=3.00
PRIMER_PAIR_35_PRODUCT_SIZE=815
PRIMER_PAIR_36_PENALTY=7.824728
PRIMER_LEFT_36_PENALTY=7.069187
PRIMER_RIGHT_36_PENALTY=0.755541
PRIMER_LEFT_36_SEQUENCE=GGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_36_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_36=896,23
PRIMER_RIGHT_36=1790,20
PRIMER_LEFT_36_TM=55.931
PRIMER_RIGHT_36_TM=59.244
PRIMER_LEFT_36_GC_PERCENT=43.478
PRIMER_RIGHT_36_GC_PERCENT=50.000
PRIMER_LEFT_36_SELF_ANY=2.00
PRIMER_RIGHT_36_SELF_ANY=4.00
PRIMER_LEFT_36_SELF_END=0.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=6.7000
PRIMER_RIGHT_36_END_STABILITY=5.6000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=1.00
PRIMER_PAIR_36_PRODUCT_SIZE=895
PRIMER_PAIR_37_PENALTY=7.824728
PRIMER_LEFT_37_PENALTY=7.069187
PRIMER_RIGHT_37_PENALTY=0.755541
PRIMER_LEFT_37_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_37_SEQUENCE=GAAGGATTTGTCCCGGTATG
PRIMER_LEFT_37=894,23
PRIMER_RIGHT_37=1790,20
PRIMER_LEFT_37_TM=55.931
PRIMER_RIGHT_37_TM=59.244
PRIMER_LEFT_37_GC_PERCENT=43.478
PRIMER_RIGHT_37_GC_PERCENT=50.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=4.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=5.6000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PAIR_37_PRODUCT_SIZE=897
PRIMER_PAIR_38_PENALTY=7.840894
PRIMER_LEFT_38_PENALTY=7.455139
PRIMER_RIGHT_38_PENALTY=0.385756
PRIMER_LEFT_38_SEQUENCE=AGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_38_SEQUENCE=GCCTGAAGGAAGGACAGTGA
PRIMER_LEFT_38=895,23
PRIMER_RIGHT_38=1705,20
PRIMER_LEFT_38_TM=55.545
PRIMER_RIGHT_38_TM=60.386
PRIMER_LEFT_38_GC_PERCENT=43.478
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=3.00
PRIMER_RIGHT_38_SELF_ANY=4.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=6.4000
PRIMER_RIGHT_38_END_STABILITY=6.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=3.00
PRIMER_PAIR_38_PRODUCT_SIZE=811
PRIMER_PAIR_39_PENALTY=7.861647
PRIMER_LEFT_39_PENALTY=6.667981
PRIMER_RIGHT_39_PENALTY=1.193666
PRIMER_LEFT_39_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_39_SEQUENCE=AGAAGGATTTGTCCCGGTATG
PRIMER_LEFT_39=893,24
PRIMER_RIGHT_39=1791,21
PRIMER_LEFT_39_TM=57.332
PRIMER_RIGHT_39_TM=60.194
PRIMER_LEFT_39_GC_PERCENT=41.667
PRIMER_RIGHT_39_GC_PERCENT=47.619
PRIMER_LEFT_39_SELF_ANY=5.00
PRIMER_RIGHT_39_SELF_ANY=4.00
PRIMER_LEFT_39_SELF_END=0.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=5.6000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PAIR_39_PRODUCT_SIZE=899
PRIMER_PAIR_40_PENALTY=7.908962
PRIMER_LEFT_40_PENALTY=7.025575
PRIMER_RIGHT_40_PENALTY=0.883387
PRIMER_LEFT_40_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_40_SEQUENCE=TTTGGAAGAAGAAGGTGCGA
PRIMER_LEFT_40=894,24
PRIMER_RIGHT_40=1675,20
PRIMER_LEFT_40_TM=56.974
PRIMER_RIGHT_40_TM=60.883
PRIMER_LEFT_40_GC_PERCENT=41.667
PRIMER_RIGHT_40_GC_PERCENT=45.000
PRIMER_LEFT_40_SELF_ANY=5.00
PRIMER_RIGHT_40_SELF_ANY=2.00
PRIMER_LEFT_40_SELF_END=0.00
PRIMER_RIGHT_40_SELF_END=0.00
PRIMER_LEFT_40_END_STABILITY=6.4000
PRIMER_RIGHT_40_END_STABILITY=10.2000
PRIMER_PAIR_40_COMPL_ANY=4.00
PRIMER_PAIR_40_COMPL_END=3.00
PRIMER_PAIR_40_PRODUCT_SIZE=782
PRIMER_PAIR_41_PENALTY=7.924554
PRIMER_LEFT_41_PENALTY=6.730887
PRIMER_RIGHT_41_PENALTY=1.193666
PRIMER_LEFT_41_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCTT
PRIMER_RIGHT_41_SEQUENCE=AGAAGGATTTGTCCCGGTATG
PRIMER_LEFT_41=894,25
PRIMER_RIGHT_41=1791,21
PRIMER_LEFT_41_TM=58.269
PRIMER_RIGHT_41_TM=60.194
PRIMER_LEFT_41_GC_PERCENT=40.000
PRIMER_RIGHT_41_GC_PERCENT=47.619
PRIMER_LEFT_41_SELF_ANY=5.00
PRIMER_RIGHT_41_SELF_ANY=4.00
PRIMER_LEFT_41_SELF_END=0.00
PRIMER_RIGHT_41_SELF_END=0.00
PRIMER_LEFT_41_END_STABILITY=6.7000
PRIMER_RIGHT_41_END_STABILITY=5.6000
PRIMER_PAIR_41_COMPL_ANY=3.00
PRIMER_PAIR_41_COMPL_END=1.00
PRIMER_PAIR_41_PRODUCT_SIZE=898
PRIMER_PAIR_42_PENALTY=7.924554
PRIMER_LEFT_42_PENALTY=6.730887
PRIMER_RIGHT_42_PENALTY=1.193666
PRIMER_LEFT_42_SEQUENCE=AAAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_42_SEQUENCE=AGAAGGATTTGTCCCGGTATG
PRIMER_LEFT_42=893,25
PRIMER_RIGHT_42=1791,21
PRIMER_LEFT_42_TM=58.269
PRIMER_RIGHT_42_TM=60.194
PRIMER_LEFT_42_GC_PERCENT=40.000
PRIMER_RIGHT_42_GC_PERCENT=47.619
PRIMER_LEFT_42_SELF_ANY=5.00
PRIMER_RIGHT_42_SELF_ANY=4.00
PRIMER_LEFT_42_SELF_END=0.00
PRIMER_RIGHT_42_SELF_END=0.00
PRIMER_LEFT_42_END_STABILITY=6.4000
PRIMER_RIGHT_42_END_STABILITY=5.6000
PRIMER_PAIR_42_COMPL_ANY=3.00
PRIMER_PAIR_42_COMPL_END=0.00
PRIMER_PAIR_42_PRODUCT_SIZE=899
PRIMER_PAIR_43_PENALTY=7.952573
PRIMER_LEFT_43_PENALTY=7.069187
PRIMER_RIGHT_43_PENALTY=0.883387
PRIMER_LEFT_43_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_43_SEQUENCE=TTTGGAAGAAGAAGGTGCGA
PRIMER_LEFT_43=894,23
PRIMER_RIGHT_43=1675,20
PRIMER_LEFT_43_TM=55.931
PRIMER_RIGHT_43_TM=60.883
PRIMER_LEFT_43_GC_PERCENT=43.478
PRIMER_RIGHT_43_GC_PERCENT=45.000
PRIMER_LEFT_43_SELF_ANY=5.00
PRIMER_RIGHT_43_SELF_ANY=2.00
PRIMER_LEFT_43_SELF_END=0.00
PRIMER_RIGHT_43_SELF_END=0.00
PRIMER_LEFT_43_END_STABILITY=6.4000
PRIMER_RIGHT_43_END_STABILITY=10.2000
PRIMER_PAIR_43_COMPL_ANY=4.00
PRIMER_PAIR_43_COMPL_END=2.00
PRIMER_PAIR_43_PRODUCT_SIZE=782
PRIMER_PAIR_44_PENALTY=7.983102
PRIMER_LEFT_44_PENALTY=6.398149
PRIMER_RIGHT_44_PENALTY=1.584952
PRIMER_LEFT_44_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_44_SEQUENCE=GAACTTGTGCCTACTCGTCGT
PRIMER_LEFT_44=892,25
PRIMER_RIGHT_44=1629,21
PRIMER_LEFT_44_TM=58.602
PRIMER_RIGHT_44_TM=59.415
PRIMER_LEFT_44_GC_PERCENT=40.000
PRIMER_RIGHT_44_GC_PERCENT=52.381
PRIMER_LEFT_44_SELF_ANY=5.00
PRIMER_RIGHT_44_SELF_ANY=3.00
PRIMER_LEFT_44_SELF_END=0.00
PRIMER_RIGHT_44_SELF_END=1.00
PRIMER_LEFT_44_END_STABILITY=6.4000
PRIMER_RIGHT_44_END_STABILITY=7.8000
PRIMER_PAIR_44_COMPL_ANY=3.00
PRIMER_PAIR_44_COMPL_END=0.00
PRIMER_PAIR_44_PRODUCT_SIZE=738
PRIMER_PAIR_45_PENALTY=7.995492
PRIMER_LEFT_45_PENALTY=7.025575
PRIMER_RIGHT_45_PENALTY=0.969917
PRIMER_LEFT_45_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTCT
PRIMER_RIGHT_45_SEQUENCE=GGATTGCCTGAAGGAAGGAC
PRIMER_LEFT_45=894,24
PRIMER_RIGHT_45=1710,20
PRIMER_LEFT_45_TM=56.974
PRIMER_RIGHT_45_TM=60.970
PRIMER_LEFT_45_GC_PERCENT=41.667
PRIMER_RIGHT_45_GC_PERCENT=55.000
PRIMER_LEFT_45_SELF_ANY=5.00
PRIMER_RIGHT_45_SELF_ANY=4.00
PRIMER_LEFT_45_SELF_END=0.00
PRIMER_RIGHT_45_SELF_END=1.00
PRIMER_LEFT_45_END_STABILITY=6.4000
PRIMER_RIGHT_45_END_STABILITY=7.6000
PRIMER_PAIR_45_COMPL_ANY=4.00
PRIMER_PAIR_45_COMPL_END=3.00
PRIMER_PAIR_45_PRODUCT_SIZE=817
PRIMER_PAIR_46_PENALTY=8.039103
PRIMER_LEFT_46_PENALTY=7.069187
PRIMER_RIGHT_46_PENALTY=0.969917
PRIMER_LEFT_46_SEQUENCE=AAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_46_SEQUENCE=GGATTGCCTGAAGGAAGGAC
PRIMER_LEFT_46=894,23
PRIMER_RIGHT_46=1710,20
PRIMER_LEFT_46_TM=55.931
PRIMER_RIGHT_46_TM=60.970
PRIMER_LEFT_46_GC_PERCENT=43.478
PRIMER_RIGHT_46_GC_PERCENT=55.000
PRIMER_LEFT_46_SELF_ANY=5.00
PRIMER_RIGHT_46_SELF_ANY=4.00
PRIMER_LEFT_46_SELF_END=0.00
PRIMER_RIGHT_46_SELF_END=1.00
PRIMER_LEFT_46_END_STABILITY=6.4000
PRIMER_RIGHT_46_END_STABILITY=7.6000
PRIMER_PAIR_46_COMPL_ANY=4.00
PRIMER_PAIR_46_COMPL_END=3.00
PRIMER_PAIR_46_PRODUCT_SIZE=817
PRIMER_PAIR_47_PENALTY=8.084109
PRIMER_LEFT_47_PENALTY=6.398149
PRIMER_RIGHT_47_PENALTY=1.685960
PRIMER_LEFT_47_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_47_SEQUENCE=CCTGAAGGAAGGACAGTGAAA
PRIMER_LEFT_47=892,25
PRIMER_RIGHT_47=1704,21
PRIMER_LEFT_47_TM=58.602
PRIMER_RIGHT_47_TM=59.314
PRIMER_LEFT_47_GC_PERCENT=40.000
PRIMER_RIGHT_47_GC_PERCENT=47.619
PRIMER_LEFT_47_SELF_ANY=5.00
PRIMER_RIGHT_47_SELF_ANY=4.00
PRIMER_LEFT_47_SELF_END=0.00
PRIMER_RIGHT_47_SELF_END=0.00
PRIMER_LEFT_47_END_STABILITY=6.4000
PRIMER_RIGHT_47_END_STABILITY=7.3000
PRIMER_PAIR_47_COMPL_ANY=4.00
PRIMER_PAIR_47_COMPL_END=3.00
PRIMER_PAIR_47_PRODUCT_SIZE=813
PRIMER_PAIR_48_PENALTY=8.100090
PRIMER_LEFT_48_PENALTY=6.398149
PRIMER_RIGHT_48_PENALTY=1.701940
PRIMER_LEFT_48_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_48_SEQUENCE=TCGATTTGGAAGAAGAAGGTG
PRIMER_LEFT_48=892,25
PRIMER_RIGHT_48=1679,21
PRIMER_LEFT_48_TM=58.602
PRIMER_RIGHT_48_TM=59.298
PRIMER_LEFT_48_GC_PERCENT=40.000
PRIMER_RIGHT_48_GC_PERCENT=42.857
PRIMER_LEFT_48_SELF_ANY=5.00
PRIMER_RIGHT_48_SELF_ANY=4.00
PRIMER_LEFT_48_SELF_END=0.00
PRIMER_RIGHT_48_SELF_END=0.00
PRIMER_LEFT_48_END_STABILITY=6.4000
PRIMER_RIGHT_48_END_STABILITY=7.9000
PRIMER_PAIR_48_COMPL_ANY=4.00
PRIMER_PAIR_48_COMPL_END=1.00
PRIMER_PAIR_48_PRODUCT_SIZE=788
PRIMER_PAIR_49_PENALTY=8.104369
PRIMER_LEFT_49_PENALTY=6.398149
PRIMER_RIGHT_49_PENALTY=1.706220
PRIMER_LEFT_49_SEQUENCE=AAAAGGTTATCTTGTCGTCTCTCTC
PRIMER_RIGHT_49_SEQUENCE=TCTTTGATTGGGGATTGCCT
PRIMER_LEFT_49=892,25
PRIMER_RIGHT_49=1721,20
PRIMER_LEFT_49_TM=58.602
PRIMER_RIGHT_49_TM=61.706
PRIMER_LEFT_49_GC_PERCENT=40.000
PRIMER_RIGHT_49_GC_PERCENT=45.000
PRIMER_LEFT_49_SELF_ANY=5.00
PRIMER_RIGHT_49_SELF_ANY=3.00
PRIMER_LEFT_49_SELF_END=0.00
PRIMER_RIGHT_49_SELF_END=1.00
PRIMER_LEFT_49_END_STABILITY=6.4000
PRIMER_RIGHT_49_END_STABILITY=9.7000
PRIMER_PAIR_49_COMPL_ANY=4.00
PRIMER_PAIR_49_COMPL_END=0.00
PRIMER_PAIR_49_PRODUCT_SIZE=830
=
SEQUENCE_ID=MAGIC.338_2_5923596_5923823_kn-0_bur-0_13830_5564_TRUE
SEQUENCE_TARGET=950,327
SEQUENCE_TEMPLATE=GAGANANTCCCNTCTCNNAAAANAAACCTACGTCAAAACGCAGCCACCAAAACNTTGTAATNAGAAAAACNTTTTACTNTGGTAACGCTTTANTCCTAACTTCACTANCAGTAATGAGTGCTGCTGCAANCACAAGACACACCTCGTGGATGGTGGCTACGGCNATCGCNGCCGTGGAANCTCGTTNGAACTATCCTCTCCGTTNTTTCAACAAAGATGTNAGGGCTCGTCTTCGTGCCATCGCTGTCACTTCNCGTNCACCTTCNTCCNNNNNNTCTTCTTCTTCTTCTTCGGCTGANNTAGTCAAGGANAATCATCCTATGCCTAAATCTGAAGCTTCCATGGAAAGAGTTATGNGTCTTAGCTGCNTNGGNCCAACCACTGTTCGATTCTAGATCTCTTCCTTTGTATGTGACTNTTTTTCNNCCATNGTTTTCTTGNATTTNATTANNTNANNTTCTTGTGGTAGCTAAGTCATGAATTACAAATCATATAGTTTGATTATTTNAGGCAAATCAATCNCTTNTNTTGGCCANANTTTTTTACAANATTCTCCCANGAAGAGNTGANACNANTTTNGTANATCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATNTGTTGACATTTCTAGGCAAGTNATNCTAGCTCAAAGGAGNTTTATTTAGAAGAAAGAAANNCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTATTTACNTTNTCCNAGANCAATCAAAATGNNTNNTTTTTCTATAAGATGAAANTTATAGAGNTTNCACNTCGGCAANNNTAGATAATTTCATCAATATTCATTACACTCATATANANTNTTATNTNNNNNNNTTGNTNGTCTCATAATGTGNGGCTTGTGATGTTTGNTTCAATACCTTGTCNCAGATTAAACNTCAGACGAGNNNTNAGGGATNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCATAACTNATTTTCTNAAAATATTTTGAAANTNACATACGGATGATCTCNTNAATTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCAAATTNNGATCCAAACCGGANAANNNTGTATTGTNNNANTTTAAAATNTCCAAACNGGCTTTCCTTCGNTTNNCNGTTAAANNATCTAATTTTNGNTTTNAAANTATAATTTNAGAACTCNNACGTTTTNTNNNNNNNNNNNTATGTNTTGNTCCTCGAGTTTACAATCNTCGNTTCNATAATCNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNGNATANNTATTAAATNNTNTTCTAAAAAGCGATTTANNNNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCNTAANATCNTATATANNTCGNTTTANTATATNTTTNTANTTTTNAANNAATTTTATATTTNTATAAATAGTTATAAATGTTNATATAGTANNTNATATAGTTAANNNAATATTTTGTATANNTTNNNTTTANGATTNNACNNNNNNNNATATANNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.446_4_4739079_4739625_ler-0_can-0_8931_2734_TRUE
SEQUENCE_TARGET=950,646
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.287_1_16435614_16435661_zu-0_bur-0_343066_999_TRUE
SEQUENCE_TARGET=950,147
SEQUENCE_TEMPLATE=TTGATNNACACGTCCAATATGTTCTGTATTCAGTTNNATCGACCGACNAAGATGACNAGCACAATGGAGCTTAGNCAGCGTGGATTTTTCGAGCCTGAAAGATTTAGCCGTATATTAGGATATATGATCCTCACCNAATTCCCTATTGATANGTTTTACATTTTACTCTCTTNTTCCNAAATATNTGCTAACTAACNAAATNTAAGCCNCAANATGTAGATGTTGATAGATTTTTCGTNNATATTGNTGNCCTCAAAAGAATCCCTTACGNTTGTGGTCTATTCGNTAGGAAAAATGGATGCAANTNTGTGTATTTCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGTTTGTTTGGGCTGCAAGGAATGTAAGGAATGTACAATATTCNTAATCGGATNATTGAGNNNNNNNNNNNNNNCTANCAAAATANTTGCTGANTTTCGTGATGATTAGTCATTTGTAATAATTNTGNGATANGTTAAATTCNATAAAATATTGTTAACATTCNTNATTTGATTTTCNTATCNATATAATTNAAGAGACAACGAATTAAAANNTAAANNTGNAAAATATNNTTTAATNTTNNANATNNATAANTNNNNTAANNTTTTNTATGNATNANTNTNNNNTTTTAGGGAAANCATGTNTNNTGNATTNNNTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATNNACGANATGAANNAANTNTTGAATNANNNNATTNAGTNNTNANNNNNATNNAAANTNTNGAATNNNTTTNNCNTTTTANNNCNNAANNTNTATNNANAAATTNNNNGTANTANNTTTNNTANNTNTTANNTNCACNAANCNNTNNNNAANNNTNNTANTNNANTTNTNTTANCNNNATATTNTNTTANNNACNAAANNCNNNNNNTNANNNNNCNNGTNTTTNATANNNNNNAANNNATNNNNTNAANNTNNNTNAATACNNNANNACTNNNNGTANATNNNTTTATNTTTNAAAANTTATAGAATCANNNTAACNCAATANNNTTNCNNNATNANNNTTTNTGTTTNNNNNTTTNTNGANNNAAANAANNNNANATTNTNNNAATNATTAANATTTNTNNNNNNNNNCATCTTAAANCCNAATANTCTNTCCATNATTAATAAAACAAAACTCCANAAANATTAANACATATCTCAAAATGTTCTATTCTTGATTTTGAAGCNTTCCAAGTGAATCAAGAGCTTCGGTGCGAGTACGAACAATTTGGTGGAAGACATCCCTCACTTTCCTATCAAATGGATCTAAACCCTCCTTTATAGCTTCACACNCATTCATCAATTCTTGAACTCTCTCCTTCACCTCTAANGTANNNTTTTCATCNGTTAATGAGAAATTGTCCGAATCAATCAAATCACTTAA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.433_5_19750774_19750816_hi-0_ler-0_335953_404509_TRUE
SEQUENCE_TARGET=950,142
SEQUENCE_TEMPLATE=AAGCNTAGCCTTCCTTCTCACCCGGAAGACTTTAACCCCTTCNTCATNCTACATAAATCCACNCATGTTATATTAAGTCATGCATGCTAATATAGAAATTGTATAGTTTCAACAATCAAATCTTACTTGACCCTTCACATTCAGAGTAACATGAGTTGATTCCGACTCAACGTCTTTACGCTTTTCCCNTTCCATCNTCACTTCATTNCTTCCATGAATACTACTGCTCTTCGTNGACATTATCTCCNCTGGTTGGATCAGATAATTCAGTTCTNAAGANNNNNNNNNNNNNNNNNNNNTNNTTNNNTTGTTGCTAAAAGGTANGANTTAATCNTNGTATATTTATACAAGAAACTGANGAAGANNGAAAGACNAGCGANACAATACTCCATCNGTCAAAAATTTATGGTCGACCAATTATTACTACTCCCAATGTTAATTNTCTATGNCATTCTTTTAGCCAAAACTTCATTGATTAAGGCTNTGANTGGGAGGGAAGCAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.329_3_14038327_14038413_ler-0_ws-0_45929_291214_TRUE
SEQUENCE_TARGET=950,186
SEQUENCE_TEMPLATE=NTNCAGATNTAAAAGNTTANCANCCCAATCTAAGCCTGATTCACTCAAANNCANNGNNNNGNNNTAANANTCCTCGAAGTGTTGAGGGTTAAAGGACAAGGTGGGAAGAGNAGCAAGGTGGCCTCTTTTCTCAGCTAAAACAGCCTCCAGTTTGGACCTTTCTTGTTCCTCAGAGAAATCTTCCTCAAGGGTTTGGTCCAACANAAGCAGGNTNCTTTCGTGCTNNTNNNNNNNNNNNTNNAGCTCCGATNTGGCNNTCTCNGTTTGAATGANNAGGATAGCNCCTTGNATCANNTCCATNCCGCGACNTTTCACNTCTCTTNNNNNNNNNNNNNNNNNNNTGGCTAATTCAGCATCNNANNNNNNNNNNNNNNNNTTGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNAGTCGAGAGTTCNTCCTNNAGCTTGTCNTANNTTTGNTACTGATCNTGCAACTCGCCATTNGANNTCATGAGCTTNNNNNNNAGATGNTCGNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAGAGGAGGAGCNNNNNNNNNNNNNNNGNGAAGANAANTTGCCTTTCCAAGCATCNATNTCCNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNGATCATGTNNTTAAGCTCNNTGTTCGCCTNAGCAANAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAACCCGANATGAATGATGNGCNTGGAGTAAANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGATNGTGTNTTTCCNNGTTTTGAAGCNNNGNAANACGTTGCCCTCTTTCTCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGCGAGNGAAATNAANCCNTNGTTNGAACCTNTAAGCNATTTNGTNTGAGGAACGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNTNGATCAGNCTTCTCNCCACTAGGTNGNNGAGAAGGNNNNNNNNNNNNNNNNGAGGAGGAGNACCCATCGATCNAGATGGAGGAGATGATCGNNGAGAGCGATCAGTNCGAGCCNTTGACTTTTCCCTCCAAGGAGAAGAGCTGTTNCGAGCTGGTCTCTCNCTCNNTNTNNNNATNAAGTCAGCGATTGGCTTGCTGCTCAGTCCATCACGGGCTNAAGTCCCGTGTGGTNTGGGAGGANGNGGTGGAAGATGATGGAGTTGTCCAGGAGGACTCATNTNGAGNNNNNNNNNNNNNNNNNNNNNNNNGTCGAGGGTAAACAAATTCCAAAGGGTTTCACCTTCGATGACCACTTTGAAGAANNNNNNNNATTCTTCAGTCGGAAGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGAACAGAAGANANATATGNTTNTGCCCTAATTTCTACCAGGTTTNNTGGNCCANTTTGATGGAAGCACTTCGNAAAGGCTNNCAATNGAAGCAANGGNNNNNTTNATTAAGAACTANGGATGGTGNNANNNNNNNTNTTTGTTCGGNAGATNGGANATGAGGTTNNTGTTNGCATTNNNGTANGCNNAGAANTAACCNANTTTCTTCGANNAACTTCTTACNCTNAGAAGCTCNTTCAATTNGGNAACTNCNATTATGTACCCAGCNNCCNCNGCGACGGTNATNANCCCTAGAATAGNTNGGAGAAGGTTGGNGGTTAANTGNGGNAGANCNATTTCAAGAGCNGCCAAGNACNGAACGAGNGCCTNGGGANNAGNANAGGNNAGTCNGNANACCGCGAGGTGAATGAGAAGTCNGGATTAAAGGTGGTTNGATTAAAGGGCCNTTGGGCTGGAANGNGNNNNNCCNGGAAGCNCGAAGTCAATTCAAATTCGAACGTNTGAACANTNAATTCGAAGATCGAAACGGNTNCATTTAAGANNNAATAATTNTGTGTA
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=MAGIC.175_1_14265550_14265721_ct-1_wil-2_13907_49313_TRUE
SEQUENCE_TARGET=950,271
SEQUENCE_TEMPLATE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=